LOCUS       DASPRR010000001         5851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_967911, whole genome shotgun sequence.
ACCESSION   DASPRR010000001 DASPRR010000000
VERSION     DASPRR010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967911"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VEL68_00005"
     CDS             <1..316
                     /locus_tag="VEL68_00005"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34377.1"
                     /translation="IAEARKKFKVVYHPEYATRYFAGEGRIDLKLKNGKVLTGAMDQP
                     YGGPKYPLTMDEVVAIYRKYLKGILSEKHIERTKDIILNLENEPDIKELADICTFRHM
                     VK"
     gene            445..2400
                     /locus_tag="VEL68_00010"
     CDS             445..2400
                     /locus_tag="VEL68_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HXZ34378.1"
                     /translation="MGVFGEITRVLREQGMSSEGMAQARKLSGLYEIIMKAMAASFSF
                     FFLYTTYFGLVSQETHVGFYYLGTFVLCFMLYKAGKKSPENRVSLLDGLFIAGMIVTI
                     VYYVIEFPTLADRLGGATATRDVIFGWFLIILSLEMARRSSGAVIPMIGVALLIYAYF
                     GPYFPLGLGHSGFSLARIAESLFLTGDGILGMICNIFASYIFIFVILGSFMEVSGCGK
                     AFVDLAYSITGKRTGGPGLASVCTSALFGTVSGSAVANVVVDGVFTIPLMKRLGYPPH
                     FAGAVEAATSTGGQYMPPVMGAAAFLLAEISGTPYVEVIKIAAIPACLYFASTGVIIY
                     LEAVKRGLKGMPASELPQIRELMKSIHLLLPIPILIALLALDITPFIAAFYAICATVV
                     VSWVRKETRMGPKKVYEALVAGARSSLSVGATVGVIGIVIGVTNLTGLANYFQQFVIY
                     LSGGHLFLLILLITIAGLFIGMGLPTTPSYVVLVILGVPALIKMGVAPLTAHLLVFWV
                     AVQSAVTPPVALAAWAAAAIAKSDPWKTGWTATKIASWIYLMPYLFVYTSILNIGWNF
                     EFITTVASCIVALIAWGAALEGHWFKETTLFERVALFAAAFGLLFESVVTDLVGLGLV
                     ILVMVLQKGSIAKAKKVSLGVDIANPK"
     gene            2536..3540
                     /locus_tag="VEL68_00015"
     CDS             2536..3540
                     /locus_tag="VEL68_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ34379.1"
                     /translation="MEKKIWVFALVAIFCCGMMGVGVVGAAEVQKYIMGSGPMGGPWR
                     IGVGAGVQLINEQLKDKYFFTAAASGGSVENVRRMVAGEYHTAWAHINNMYDAWNGVG
                     LFEGQKPYKEMRVLEYMIDQALCVATLAKSPIKNFTDLAGKKVNLGPAGSGGVPIARA
                     IFKALGLTDKVKLNYLNFEGAAQALKDGQMDVTISPGGPYVTPSIVEISRSVPIRLLE
                     PSSEEAKKVEAELSYLYQGVIPPDKAPGENADRERKAFFWAMYWVAQASMPDQAVYDV
                     LKVTQEPKNKDLLGKVLNYWISAGPNFSSIKKIGIPIHPGAVKYWKEKGAPIPAELIK
                     "
     gene            3557..3913
                     /locus_tag="VEL68_00020"
     CDS             3557..3913
                     /locus_tag="VEL68_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ34380.1"
                     /translation="MGSAVVREDEREFARVDWGRTKNLFGPENVGAQYLKINITEYAP
                     GTEHTLHRHPDQEEVIYVLEGEGISRTKAGDQSIRAGSFVFVPSDTDHATINLRKDKP
                     MRAIIIKSPPQEKGGK"
     gene            4212..4589
                     /locus_tag="VEL68_00025"
     CDS             4212..4589
                     /locus_tag="VEL68_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ34381.1"
                     /translation="MKYFYDRFEEYTWQYIADTHSDSVKAEILIDIPPAKEFRIHITR
                     FRPGAGADPHTHEWEHAMYIMQGIGKVVIQGEEALVKKGMLAFVPRDAVHSIVNIGND
                     ELVVWGVSGPPKTEAGYAQLKKM"
     gene            4648..5103
                     /locus_tag="VEL68_00030"
     CDS             4648..5103
                     /locus_tag="VEL68_00030"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ34382.1"
                     /translation="MIKDRVGGLIFLLTGFYGIIYSHGLPMGRWNEPGPGVFPFGLAI
                     MLAFSGIMIMARGEKTKEEKQEGKRQELLHKLLTPLLIVLLTLGFILILERIGYVASV
                     VVYLFILFLGVCRYKFLLAAGMAGVFGLGSWYFFVKVLGVQLPLSLWGI"
     gene            5130..>5851
                     /locus_tag="VEL68_00035"
     CDS             5130..>5851
                     /locus_tag="VEL68_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ34383.1"
                     /translation="MDILGNLLAGFQTALTLHNIYLCFVGCLFGTMVGVLPGIGPLAG
                     ISLLIPATYGLDATGAIIMLAGIYYGAMYGGSTTSILMNIPGESASVITCVDGYQMTQ
                     KGRGGAALFISAWGSFIGGNLSILGLMILAPLLANFATRFGPPEIFAVLVLAFILLGS
                     LGSGSFFKTMPMILLGMFLGTIGMDPLTGSFRFTHEVKDLYDGIGFVPVAMGAFGVGE
                     ILTAVEESLVRIIHKVKMRELLP"
BASE COUNT         1266 a         1447 c         1677 g         1461 t
ORIGIN      
        1 aatcgccgag gccagaaaaa aattcaaggt ggtctaccat ccagaatacg ccacgcgata
       61 ttttgcagga gaggggcgga ttgacctgaa gttgaagaac gggaaagtgc tgaccggggc
      121 catggaccag ccttatgggg gacccaagta tcccttgacc atggacgagg ttgtggcgat
      181 ttaccgcaag tatctaaaag gaatcctttc ggagaaacat atcgagagga ccaaggacat
      241 catcctaaac ctggagaacg aaccggacat caaagagctg gctgatattt gcaccttccg
      301 gcacatggtt aaataattaa ttgtatacgc aggtgggggc gggttttaaa ctcgccccta
      361 cagacgtacc ttaaccggca tgctcgccat ttatatgcgc gtcttataaa ggcgatctgt
      421 ataactgcac tccgggagtt gatcatggga gttttcggag aaatcactcg cgtcctccgt
      481 gagcagggga tgagcagcga gggcatggcg caagcccgca aattaagcgg gctatatgaa
      541 attatcatga aggccatggc cgcttccttc agcttttttt tcctttacac cacttatttc
      601 gggctggttt ctcaggaaac ccatgtgggg ttttattacc tgggtacttt cgtcctttgc
      661 ttcatgctct acaaagctgg caaaaaatca cccgagaacc gggtcagtct tctggacggt
      721 ctgtttatcg ccgggatgat tgtgaccatt gtttattacg tgatcgaatt tcctaccttg
      781 gccgaccggc ttggaggagc gacggccacg cgggatgtca ttttcgggtg gttcctgatc
      841 atcctgtccc tggagatggc caggaggagt tcaggggcgg tcattccgat gatcggggtg
      901 gcgttgctga tttatgccta ttttggccct tattttccgc tggggttggg tcactcaggg
      961 ttctccctgg cgcgcattgc cgagagcctc ttccttaccg gggatggaat tctaggcatg
     1021 atctgcaaca tcttcgcctc ctacatcttt atcttcgtga tcctaggttc tttcatggag
     1081 gtttccggtt gcggcaaggc ctttgttgac ctggcttact cgatcactgg aaagaggacc
     1141 ggggggccag ggttggcttc ggtttgtacc agcgccctct tcgggaccgt gtcggggagc
     1201 gcggtggcaa atgtggttgt ggatggggta ttcactattc ccctaatgaa gcgcctcggg
     1261 tatccccccc atttcgcggg ggccgtggag gcagccactt ccacgggcgg ccagtacatg
     1321 cccccggtca tgggagccgc tgccttcctg ctggccgaga tttccggcac cccctacgtg
     1381 gaggtgatca agatcgcggc cattccggca tgtctttatt ttgcttcgac cggagtaatc
     1441 atttacctgg aggcggtgaa acgcggcttg aaagggatgc ccgcatcgga gcttccccag
     1501 atcagagagc tgatgaaatc catccacctc ctattgccga tcccgattct cattgctctc
     1561 ctggccctgg acatcactcc attcatcgcc gccttctatg ccatctgcgc tacggtagtg
     1621 gtcagctggg tcaggaagga aacccggatg gggccgaaga aggtttatga agccctggtc
     1681 gcgggggctc gttcttccct ttctgttggg gccactgtag gggtcatcgg catcgtcatc
     1741 ggggtcacca acctgaccgg tctggccaac tattttcagc agttcgtcat ctacctgtcc
     1801 gggggacatc ttttcttgtt gattctcctc atcaccattg ccggcctttt catcggcatg
     1861 ggcttgccca caaccccttc ttatgttgtc ctagtcatat tgggagtgcc ggctttgatc
     1921 aagatggggg tcgcgccctt aaccgctcac ctcctcgtct tctgggttgc cgtccagtct
     1981 gcggtcaccc cgccggtggc cttggcagcc tgggctgctg cggccatcgc caagtcggac
     2041 ccctggaaaa caggctggac ggccacgaag atcgcatcct ggatctacct catgccttac
     2101 ctgtttgtct atacttccat tctcaacatt ggttggaatt tcgaattcat tactaccgtg
     2161 gccagctgca ttgtggccct gattgcttgg ggggctgccc tggaggggca ttggtttaag
     2221 gagaccactc tgtttgagag ggtggccctc ttcgcggctg cttttggcct gctgtttgag
     2281 agcgttgtta ccgatctcgt cggcctagga ttagtcatcc tggtcatggt gctccagaag
     2341 ggttcaatag ccaaggcgaa aaaggttagc ctgggggttg atatagcgaa tcccaaatga
     2401 aaaggcccag aacggaggca gtcaagtttt aaaagagaaa caggccaaac ctacccttgg
     2461 cctgatccag catgaatggg ggctgagcca agggatggca tgttgagttc ccgaaatttt
     2521 caaaggaggt ggtttatgga aaagaaaatt tgggtttttg ctcttgtggc aatcttctgc
     2581 tgcggcatga tgggggtagg ggtcgtgggg gctgcagaag ttcagaaata catcatgggc
     2641 agcgggccca tgggcgggcc gtggcgaatc ggagtggggg cgggggttca gctgattaat
     2701 gagcagctga aggataaata ctttttcacc gccgcagctt cgggagggtc ggttgaaaac
     2761 gtacgtcgga tggtggctgg ggaataccac accgcctggg cccacatcaa taatatgtat
     2821 gatgcctgga acggcgtagg gctcttcgaa gggcagaagc cttacaagga aatgagagtc
     2881 cttgaatata tgattgacca ggcgctatgc gtggccaccc tggccaaatc cccaatcaag
     2941 aattttaccg atctcgcagg gaagaaggtc aaccttggcc ctgccgggag tgggggagtg
     3001 cccattgcca gggccatttt caaggctctg ggcctgaccg ataaagtgaa actgaattac
     3061 ctgaattttg aaggagccgc tcaggcgctc aaggatggac agatggacgt cacaatcagc
     3121 cccggagggc cctatgtaac tccctctatc gtcgaaattt cccgttccgt gccgattcgt
     3181 ctcctggaac catcttccga agaagctaaa aaggtcgagg ccgagttgtc ttacttatac
     3241 cagggggtga ttcctcccga caaagcaccc ggggagaatg cggaccggga gaggaaagcc
     3301 ttcttctggg caatgtactg ggtagctcag gcgagcatgc ccgatcaggc ggtgtatgat
     3361 gtccttaagg tgacccagga gcccaagaac aaggatcttt tgggaaaggt tctgaattac
     3421 tggatcagcg ccgggcccaa tttttcttcg atcaagaaaa tcggcatccc catccatccg
     3481 ggggcggtga aatactggaa ggagaaaggg gccccgattc ctgcggaact tatcaaatag
     3541 ggaaggagat cattacatgg gaagcgcagt ggttcgagag gatgaaagag aatttgcccg
     3601 ggtggattgg ggaaggacga agaacctgtt tggcccagag aatgtcggcg cccaatacct
     3661 gaagatcaac attaccgaat acgcgcctgg gaccgaacat accctgcacc gccatccgga
     3721 tcaggaggag gtaatctatg tcttggaggg agagggtatc agccggacga aggccgggga
     3781 ccagtcgatc cgcgcggggt cttttgtgtt cgttccttcg gataccgacc atgccacgat
     3841 taacctgagg aaggacaagc cgatgagggc aatcatcatc aagtccccac cccaagagaa
     3901 gggggggaag tgattacgcg atacctggtc agagaagaga aactcatcag gtgcataaat
     3961 ccatactcac gaatcacgag aaccgttgat ggggttgctg taataatgat tgccaggctg
     4021 cgtctgaata tttccagagg gctttagcct ccgtcattcc gggcttgacc cggaatccag
     4081 gttttctttc tggattcccg ccttcgcggg aatgacgcaa ttactccatt ataaattttt
     4141 gcaatgttta gggaaacttt acagaggaaa ctccggacgg gggtaagcta aatctcggga
     4201 gggaaggcag aatgaaatat ttttatgacc ggtttgaaga gtatacctgg cagtacattg
     4261 ctgataccca cagcgattct gtaaaggccg agattctcat agatattccc ccagcgaagg
     4321 aatttcgcat ccacattacc cgctttcggc ccggagctgg agctgacccc catacccatg
     4381 aatgggaaca cgccatgtac atcatgcaag gaatagggaa agtggtgatc cagggagagg
     4441 aagccctcgt taaaaaaggg atgctggcct ttgttccccg ggatgccgtt cactcgattg
     4501 tgaacattgg aaatgatgaa ctggtggtat ggggagtgag cggcccgccc aaaaccgagg
     4561 ctgggtatgc ccaattgaaa aagatgtagg ggcatctcgc gtctcgcggg acatcgtgtt
     4621 cctacgttta ggatgaagga gttttccttg ataaaagatc gtgtgggagg tttgattttc
     4681 ttgctgaccg gattttacgg gatcatctat tcccacggtc ttcccatggg aaggtggaac
     4741 gaacccggcc cgggggtttt tcctttcggt ctggccataa tgctggcctt ttccggaatc
     4801 atgatcatgg cccgggggga aaaaacaaag gaagaaaaac aggaaggaaa aaggcaggaa
     4861 ttactgcaca agctgcttac ccccctactg attgtcctcc ttaccttggg tttcatcctg
     4921 attctggaac ggattgggta tgtagcctcg gtagtggttt atctctttat tctatttttg
     4981 ggggtctgcc gctataaatt tcttctggcc gcagggatgg cgggtgtatt tgggttgggg
     5041 agctggtatt tttttgtaaa agttcttggg gtccaactgc cccttagcct ttgggggata
     5101 tgatctcctc gttctaaggg gagttcgcca tggatattct tggcaacctg ctcgccggtt
     5161 ttcaaacggc cctcaccctg cataatattt atctttgttt tgtcggctgc ctgtttggga
     5221 ccatggtggg tgtcctgcca ggcattggtc ccctcgccgg gatctccctg ctcatcccag
     5281 ccacctatgg cctcgatgct accggggcca tcatcatgct ggctgggatt tattatggcg
     5341 ccatgtacgg aggatcaacc acctcgatcc tgatgaacat ccccggggaa tcggcttcag
     5401 tcatcacctg cgtggacggc taccaaatga cccagaaggg tcgtggcggg gcagcccttt
     5461 tcatctctgc ctggggatcg tttatcggag gaaacctgag catcctgggc ctgatgatcc
     5521 tggccccact gctggccaat tttgccactc gttttggacc cccggagatt ttcgccgttc
     5581 tcgtgctggc cttcattctt cttggatcgt tggggagcgg ctccttcttt aaaaccatgc
     5641 ccatgattct cctcgggatg tttctgggaa cgattggcat ggacccgctg actgggagct
     5701 ttcggtttac ccatgaggtc aaggatctct atgacgggat cggtttcgtt ccggtggcca
     5761 tgggagcttt cggcgttggt gaaattttga ccgctgtcga ggagagcctg gtccggatta
     5821 ttcataaggt caagatgcgg gaacttttac c
//
LOCUS       DASPRR010000002        19703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_536, whole genome shotgun sequence.
ACCESSION   DASPRR010000002 DASPRR010000000
VERSION     DASPRR010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19703)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19703
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VEL68_00040"
     CDS             complement(<1..839)
                     /locus_tag="VEL68_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ34384.1"
                     /translation="MKKGTGQESSKAPSREASGEIRKARPFWKRASRNVGAVLGIALL
                     VMVTLVGIFAPQISPYDPLAIDISQKFQPPSRQHFLGTDELGRDMFSRVVMGTRISFQ
                     VAGEVLLIAGTIGIFLGILAGYRGGIVDEIIMRAADIFLAFPSFLLAMAIVAALGASI
                     QNAILAIAIAWWPRYARLLRGQVLAVKNMPFVDAARSIGAGDFRVMLRHVLPNCLAPL
                     VVQFTTDAGAAILSTSALSFVQLGARPPMPEWGLMISQARNFIVNYWWIPTFPGLAIA
                     LAVG"
     gene            complement(858..1865)
                     /locus_tag="VEL68_00045"
     CDS             complement(858..1865)
                     /locus_tag="VEL68_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ34385.1"
                     /translation="MGKYLLRRIFTLVFVLWGMSLVTFSISHVIPVDPAAASAGMEGG
                     PEVIERIRKELGLDRPFHEQYLRYMGNILFKGDLGYSILNKRPVLQDIFTFLPASIEL
                     AVFSLLLCTVCGVLLGIFTATRAGGLVDALVRVFAVGGVAMPVFWLALLMQLVFYGIL
                     GWFPDGGRLGWEFEAPPSTTHMFLADSILAGRWPVFLDTLKHLFLPGFTLALANIAII
                     TRMTRSSLLEVLHQDYIKAARARGLSQLRVLFRHALKNALIPVVTVIGLQLGHLIAWV
                     FLVEIIFSWPGIGSYMVRSIINLDFPAVMGCTLFTSFIYVFINLAVDVVYVALDPRIS
                     Y"
     gene            complement(1966..3594)
                     /locus_tag="VEL68_00050"
     CDS             complement(1966..3594)
                     /locus_tag="VEL68_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34386.1"
                     /translation="MVKRSIGIFILILVGVAFFAGWQMFGWSKAQGAEQAAAKASSRP
                     MVWAVKTETRGLDPHQFDYDYDQKPQRASLEPLMEYTIMPDRSVKIVGILAESWKGSP
                     DARVWTFQLRKGIKFHDNTPWNAQAAKWNFERLFALNKVPATRIPQTVWPEKDGIEAV
                     DDLTLRITLKKPFAPFIETLVKKYMISPSAAKANEKNNDWGQAWCVENMVGTGPYLLE
                     KWVKGQYVSFKQNPAYWGGWGGKHAQRIILRYVPEAATHRLLLTKGDVDLSEKIELDD
                     LDEVAKVPGVVVEQHRVPRLIHLYMTEDGPFKDVRVRKAMAHAFDYDAFIKGVWKGRA
                     AKPLSPLPSAVWSFTPQPEIKKDSTLAKKLLAEAGYASGSFTVNIYILSSYGWFQPRE
                     AQILQADLKQLGIESKIMDIPDAGAFITALKKPDRGPSFYAWTFANAYDDPEDNLRRS
                     YRSDGSLNYFGYKNPKVDELIDKGATTLDQKERLRIYGELQKIIWEEYPGLFSAEEQW
                     FITRRDTLHGYDFYPFVINMSPNWYLMWVSDKTP"
     gene            complement(3658..3930)
                     /locus_tag="VEL68_00055"
     CDS             complement(3658..3930)
                     /locus_tag="VEL68_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HXZ34387.1"
                     /translation="MSKFKVEIEKILERGVCPFGFKAGDTFVFEDKGVPQTFPNFCAW
                     AFHEMTPALFTLKYGGRFPWESEPGVARISCSDSKNPVVFKITLLA"
     gene            complement(3953..5089)
                     /locus_tag="VEL68_00060"
     CDS             complement(3953..5089)
                     /locus_tag="VEL68_00060"
                     /inference="COORDINATES: protein motif:HMM:NF044937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HXZ34388.1"
                     /translation="MADWKIRSVEEAQDLVYGCCFMGTGGGGGPDIGLDLLRQALDKG
                     REIRLTDPQDLPDDAWVCTGAYVGSIAPPSEEMLALKERLGLEKRVDHEIVEAVKELE
                     SYVGIRVSAIAACELGGLNTPAPISAAAALGIPAVDGDYAGRAIPASVHLKISIAGKE
                     IWPRAFCDFAGNTTVIKQASSHAMMERIQKHLSMTTVGLIGGAGHLLKGTEMKKLINR
                     GSISRCLRIGRLLREAKGRGKEMAAFVARELDGWVLFQGVVEKKTWESKQGYMIGQYE
                     MKGIEDFRGQSFKVWFQNENMISWLNGKPYVTCPDILSVINLETGLPPTNNKIDSGDR
                     LAVLGLRAVEFYRQPAGLKVLGPSYFGFDIPYRPIESILAGKPA"
     gene            complement(5161..5460)
                     /locus_tag="VEL68_00065"
     CDS             complement(5161..5460)
                     /locus_tag="VEL68_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ34389.1"
                     /translation="MKKTTRPITVHMKLFTGAFPERISAQMKLGACVRDLMAQVKKEN
                     FKNLSRENSPMPLQQVAQDDLLLILNGRPIQNLQGYDTPLEDGDVLAVFPVMAGG"
     gene            complement(5499..7472)
                     /locus_tag="VEL68_00070"
     CDS             complement(5499..7472)
                     /locus_tag="VEL68_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ34390.1"
                     /translation="MPFAFAGKVLHVNLTTGKITPKPLDEKKVLKYLGARGFNARLLW
                     DLLPPGVDPLGPENLLIFGTGTLTGTSAPTSGRTTVSCKGPATGLYLKTSVGGHWGAE
                     LKFAGYDYLVIEGRAAKPVYLVILDGAVKIRDAAELWGKDVRETTRAIEHALGDPEVQ
                     VACIGPAGENRVLFASIMMNYYNAAARGGVGAVMGSKNLKAVAVRGHGKIRVARPQEF
                     RKAIMESIEVSYQDFSAKTSYLYGTSASVAVINELGSFPSFNFRSGRIDNAEKISGPY
                     LVEAGYLKRRMGCNACIFSCHRYCEVASGPYTGAYGGGPEYETMSALGAGCGTVDVEL
                     VIKANELCNLYGMDTISAGSVVQWAMESYERGVITEKDTGGVKLEWGSREAILSLLEQ
                     VALHKGFGARLGGGVRRAAKSVGKGSEKWAVEAKGLEQSRVETRTAFGYALAFAVNPR
                     GPDHLHSQVLAEFGTRPGGRALVAKITGHERYAFPRTLEKRADIVRWHEDCYAATDAV
                     GLCSFATTARYGVTPELLARMLSAATGIDFFESESLLLAGRRIVNLEKAFNVREGATR
                     EHDCLPHRLMNEVLPQTSAKDDDRSKAAPDRPSPVRESINSRAYLDRMLDEYYELHGW
                     EKTTGLPRCKTLGDLDLGDVAAELDRMGKLAKD"
     gene            complement(7580..7987)
                     /locus_tag="VEL68_00075"
     CDS             complement(7580..7987)
                     /locus_tag="VEL68_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ34391.1"
                     /translation="MPPKLVVRPENCTGCKSCMLICSFSHTDAFDYGEARVRVAKNES
                     RGQSSPLVCRNCEEAPCIPACPVEAIRREPKNQWVILNEDACTGCGACVDACPYQAIH
                     LQEGRGVAVKCDFCGGDPQCVKVCRLPEALVWR"
     gene            complement(8003..8530)
                     /locus_tag="VEL68_00080"
     CDS             complement(8003..8530)
                     /locus_tag="VEL68_00080"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXZ34392.1"
                     /translation="MAEETETTRMGRPVTDIDRAFNVQVRCVVVVTTKSGQKLNGMSA
                     AWVSRASEQPFLVMASIYKEHYSHDLIKTSKIFAVNYLRDGQQRLAIHFGKQSGRDVD
                     KFRKVPYFTEKTGAPILKDCLAYLDCRVINQMDSGDHTIFLGEVLAGKVLAPGRGLQY
                     DLKDYLNASASPGGP"
     gene            complement(8706..9413)
                     /locus_tag="VEL68_00085"
     CDS             complement(8706..9413)
                     /locus_tag="VEL68_00085"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXZ34393.1"
                     /translation="MEFQLRPIGKKKIYREILEEIWQSIQRYDLKPGDRLPTEGYLVK
                     QLKIARPTLREALSVLDYVGILESVQGGGYYVKSVSPIQPSVLLSRNGKSVSPYELVG
                     ARLLIEPEICRLAARERSEKDLDDLAQVLEKMREKISKGTFTSREDNSFHLGIARAAS
                     NVILQSMVETLMSLKEQILYEAIEVTAYRHNPRLRDMQLEHEKIFESIKNRKADQARD
                     AMKRHLLRIKKDFYRRP"
     gene            complement(10183..10890)
                     /locus_tag="VEL68_00090"
     CDS             complement(10183..10890)
                     /locus_tag="VEL68_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXZ34394.1"
                     /translation="MGKTKIKWLSHAGFQITSSGGKVIYIDPWFENPIGGFSLDDVKE
                     ATLILVTHDHFDHVGQAPDIVKKTGGLLVANVETGRRIQSEFHIPAEKVCYFGYGMNI
                     GGNLVYEGISVTMTQAFHSTLSGAPCGYVIKLEDGTTLYHSGDTGIFDSMKLFGQVYR
                     IDVAMLPIGSVFTMDPFQAAKATKMISPKKVIPIHYKTFPILVQDPKEFIALAKKEAP
                     KVEVLALNPGDEYTYTK"
     gene            complement(11003..11722)
                     /locus_tag="VEL68_00095"
     CDS             complement(11003..11722)
                     /locus_tag="VEL68_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34395.1"
                     /translation="MSGETWFGIRNLRVFYGRVEALKGISLEVDQGAIVTLIGANGAG
                     KSTSLRAISGLVHPASGEIVFQGKRIDSLSPQDVVRGGIAHVPEGRRVFPFMTVRENL
                     NMGAFRRKDKEEIKKNLEGVFEHFHILRERRSQRAGSLSGGEQQMLAIGRALMSQPTL
                     LLLDEPSLGLAPLMVKEVGRIIGEINQRQVGIILVEQNAHMALRIARKGYVLETGSIA
                     LQGPASDLIDNEHVKKAYLGG"
     gene            complement(11719..12483)
                     /locus_tag="VEL68_00100"
     CDS             complement(11719..12483)
                     /locus_tag="VEL68_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34396.1"
                     /translation="MILEIKDISKHFGGLAALSEVSFSLNSGEILGLIGPNGAGKTTL
                     FNVLTGFFPPTYGSVLFQGDDITGLRPDQVARKGLVRTFQSTNLFQHKTVLENLVIAH
                     HLQVQSGFWGLLGNSVQARADEGAARRGSLEILEYMNLTDLKDEMAHNLPHGHQRALG
                     VAIALAARPKLMLLDEPLTGMNPEESRRFIELIRGIRERGISLMVVEHDMKAIMGLSH
                     RIVVLNYGRKIAQGTPQEVRGNQQVIEAYLGSETVT"
     gene            complement(12593..13528)
                     /locus_tag="VEL68_00105"
     CDS             complement(12593..13528)
                     /locus_tag="VEL68_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34397.1"
                     /translation="MDKRRVVLLIILLCLIAVPAFTQDPYLLNILITTGIWTTSVWGV
                     RVIMSTGQLTLGHAAYMAVGAYASTLLTMKAGLSFWLAFPLAGLISSLVALLIGYPTL
                     RIKGVYFAIITFAFAEIIRLLIVHWPSLLGGYGGIPNVPPPSSFFSISFKTRIPFYYL
                     ILALSLVTYLVMVRIDKSRIGQVFSSIHESDTLAESIGINIMKYKVIAFCTGCFFAGL
                     SGSFYAHYFNFTSPEFFTIWQSVYCLVFVIVGGAGSVWGPPLGSFFMTLIPELLRVAR
                     ELEPVIYAVILIAVMFLLPGGLVTLPARLRGKKEH"
     gene            complement(13521..14378)
                     /locus_tag="VEL68_00110"
     CDS             complement(13521..14378)
                     /locus_tag="VEL68_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34398.1"
                     /translation="MTFSFFIQVLVNGLMLGLTYVLIASGFSLIYGIMRLLNFAHGEF
                     YMLGAFATYLLCEHLGVHYFTAMALSMVIIGVLGVLVYHFFFRPFRDEHDPSLVIALG
                     IAMLIGGLALIIFGEKDKSVAPVFSGVIQVWGATLSKERVAVIIVAIVLMAALTLYIK
                     FSKTGQAMRAVSQDREAAALQGIGVDSTFTLCMGISSALAGAAGALLAPLFYVNPFLG
                     MHAVLKALVVVVIGGLGSIPGAIVGGLLLGFVESFGNTFFGDITEILGFIIVMIVLLF
                     RPQGLFGHG"
     gene            complement(14388..15587)
                     /locus_tag="VEL68_00115"
     CDS             complement(14388..15587)
                     /locus_tag="VEL68_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34399.1"
                     /translation="MKSFLKSFWSKAVILGVVSALGIPSFPLYAAQKELKIGAIFPLS
                     GRGATWGMAAQKAITVKQKEVNAKGGLKVGADTYKLEIIWEDDKFNAAAGRMAAEKLV
                     NRDRVKFILGSQSSAVILAVQPITEPSKILFLVNSYAKEVLTPDKPYTFRMVLTSNEI
                     LQGMYPWMRKTYANLNTVAFIEPNDASGWSVEKDCQRMAGQNKYQVAFSQFYERGTSD
                     FYPLLNKMIAAKPDFVDFSGAPPGDQALMVKQMRELGYTGKSFSSTTMDPAEFCKIAG
                     VQNSEGHISNTHDLLGAYTTEGQKKYYDGYIAMYGKPFDPVTPKYQVYLDVLIQALEK
                     SGTLDTTKVMETMEKTEEWQTIFGASKFGGKEHYGIKRQIVTPVYISEIIKGNLINRG
                     TMMPALP"
     gene            complement(15676..16899)
                     /locus_tag="VEL68_00120"
     CDS             complement(15676..16899)
                     /locus_tag="VEL68_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34400.1"
                     /translation="MERKKWLALTLAWAVIFTLPLFPAESSSQETLKIGMIAPLSGPG
                     APWGAVWKRTATFEQERINASGGLPIEGKSYKIEFLFEDDKYSGASGRMAAEKLIFKD
                     KVKFIVGPISSASRQAWQEISKENNITVCGNTYTRAVMGPEHPDFFRPSVTGVEQAPC
                     YYKIITERYPLIKKVAIINRSDVTGKDSVKDSRRSCKYFGFQILTEEFYEPGTKDFYP
                     LLTRVLANKPDFIDFGSSSAGDIFLMVKQARELGYTGRTMSLALIPVKEFCAVAGKKN
                     AEGHIYNVMAPPFMSSAALEYEKEYVKRFGEWDEYAPKARAYSNALIQGIRKANSMDP
                     LQVRKTMAGMEWEELDGVYKFTCQWTYGIPHQGLSPFYFTEIRNGENSCLEVKDVRWV
                     EEMTRKYEQETPAEK"
     gene            complement(17312..18820)
                     /locus_tag="VEL68_00125"
     CDS             complement(17312..18820)
                     /locus_tag="VEL68_00125"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HXZ34401.1"
                     /translation="MKANTLRFLRLILYLIAGAMGVFLVQTFAELLSTQISLPPLSSL
                     TPAEARSLVNLLSRNLNQAMSILFIVTGMAVPLTANLYSLKFLDLFIRNPVNVAVLIF
                     IIFANISGLWTNYSLTETSVPRFQLGLMMVLTLASLLLILPYLIYVFRFLHPRTLLNL
                     LEEDVRSSLRSACHSGRAGRNRERVAETLEHIANISVRSVDRFDRNTAIEGVLTLERV
                     ARHYWKVKEELRPSWFEADQNFFLGFSSLAVDEMTASRSWMEMKLYYQFFEALRAACP
                     RMPELTSTIAKSLRKLGLEAASLENPALRELTIEYFNTFIRLTLNRHDVRSVFIIFDQ
                     YRTFAETLEGNFPKEVSEIAYYFSYYGAAAREQNLPFVVEAVAHDLGALVKRAWQSDS
                     TIREELLERFLLYDRQGKAVLPGVKKAQALLASYFLYSGQNEAATLIRRSFQGMGPEF
                     LKGIMEDLLQVTRQKYWEVSERRMNIEFVPEPQRARLREFFDGLAGNSDAGR"
     gene            18942..19088
                     /locus_tag="VEL68_00130"
     CDS             18942..19088
                     /locus_tag="VEL68_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34402.1"
                     /translation="MSDAVILPLVAPGGAGKLEHFMKERSREGPNSCWPRPSSTFEPF
                     GSGK"
     gene            complement(19191..>19703)
                     /locus_tag="VEL68_00135"
     CDS             complement(19191..>19703)
                     /locus_tag="VEL68_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ34403.1"
                     /translation="TDVKVVDTETGTKEMRPGEEGELIQKGPSVALGYWKKPEDTKEA
                     FKDGWLFTGDLGTMDENGFFYITGKKKELIKYKGYNIAPRMLEEILYKHPAVLGCAAV
                     GKKDDMAGEIPVAFVSVKEGAKVTAGELMEFVNGQVAPYKKLREVHFIEKMPLFATGK
                     LNRRALIEML"
BASE COUNT         4300 a         5710 c         5330 g         4363 t
ORIGIN      
        1 cccacagcca aggcaatggc caacccggga aaggtgggaa tccaccagta gttgacgatg
       61 aaatttcgcg cctgggagat catcagcccc cattcgggca tgggcggccg ggcccccagt
      121 tgaacgaaac tgagggccga ggtactgaga atggcggcac ccgcatcagt ggtgaactgg
      181 accaccaggg gagccagaca gttgggaagc acatgccgca gcatgacccg gaagtctccg
      241 gccccgatgc tgcgggcggc atcgacaaaa ggcatattct taaccgctag cacctgtccc
      301 cgcagcaagc gggcgtagcg gggccaccac gcaatggcga tggccagaat cgcattctgt
      361 atgctggccc ccagggcagc cacaatggcc atagccagaa gaaaagacgg gaaggccaga
      421 aaaatatccg cggcgcgcat gatgatctcg tccacgatcc cgccgcggta cccggcgagg
      481 atgccaagaa aaatcccgat cgttccggcg atcagcagta cttcaccggc cacttgaaag
      541 gagatcctcg tgcccatgac cacccggctg aacatatccc ggcccaattc atcggtaccc
      601 aaaaaatgct ggcgggaggg cggttgaaat ttttgggaaa tatcgatggc caggggatcg
      661 tacgggctga tctgcggtgc aaaaatcccc acgagggtaa ccatcaccaa cagggcaatc
      721 ccgagaaccg cccccacgtt acgggaagcg cgcttccaga aagggcgggc tttgcgaatt
      781 tctcccgaag cctcccggga aggcgccttc gatgattctt gaccggtgcc tttcttcatt
      841 ggatacctct tcgccggtca ataggaaatg cgcggatcga gcgccacata gaccacgtcc
      901 acagccaggt tgataaacac atagatgaac gaggtgaaca gggtgcagcc cataaccgca
      961 gggaaatcca ggttgatgat ggaccgcacc atataagacc cgatccccgg ccaggagaag
     1021 atgatttcca ccagaaaaac ccaggcaatg agatgcccca gctgcagtcc gatgaccgtg
     1081 accaccggaa tcaaggcgtt cttcaaggca tggcggaaca gaactcgcag ctgggacaac
     1141 ccccgggccc tggcagcctt gatatagtcc tgatgcagca cctccagcag gctcgatcgg
     1201 gtcatacggg tgataatggc gatattggcc aaggccaggg tgaatcccgg caggaacaga
     1261 tgtttaaggg tatccaggaa aaccggccat cgccctgcca gaatactgtc ggccaaaaac
     1321 atatgggtcg tgctgggggg ggcttcaaac tcccatccga gacgccctcc gtccggaaac
     1381 caacccagaa tgccgtagaa gaccagctgc atgagcagcg ccagccagaa taccggcatg
     1441 gctacccccc ccacggcgaa tactcgcacc agggcgtcca ccaaaccgcc cgcccgggta
     1501 gcggtaaaaa ttcccaaaag gactccgcag accgtgcaga ggagcaggct gaagactgcc
     1561 agttcgatgc tggccgggag aaaggtgaaa atgtcctgaa gcacgggccg cttgttaagg
     1621 atggagtacc ccaggtctcc cttgaacaaa atgttcccca tgtaacgaag atactgctca
     1681 tgaaagggac ggtcgagccc cagctctttg cggatccttt cgatcacttc ggggccgcct
     1741 tccatgccgg cggaagccgc cgccgggtca acggggatta cgtgggaaat ggagaaggta
     1801 accagggaca tcccccagag gacgaagacg agggtgaata ttctgcgcag taaatatttt
     1861 cccatccaac agcaccatcg tcacccccgc ccttcccctc ccccgtcggg ggaggagaat
     1921 tacgcgggag agggccgggt gaggggtcag gccccttccc gtgcctcagg gggtcttgtc
     1981 ggagacccac atgaggtacc agttagggga catgttgatc acgaaggggt agaaatcata
     2041 cccgtggagc gtatcccgcc gggtgatgaa ccattgttcc tcggcgctaa aaagcccggg
     2101 gtactcttcc catataattt tttggagttc cccgtatatt ctgagccttt ccttctggtc
     2161 cagggtggtc gctcccttgt cgatcagctc gtccactttc gggttcttgt atccaaaata
     2221 gttcagggaa ccgtcggagc gatagctccg ccggaggttg tcctctggat catcgtaggc
     2281 gttggcaaag gtccaggcat agaacgacgg tccgcggtcc ggtttcttta aggcggtaat
     2341 aaaagccccg gcatccggta tgtccatgat cttcgattcg atccccaact gcttgaggtc
     2401 ggcctggagg atctgggcct cccggggctg gaaccagccg taggaggaaa ggatataaat
     2461 gttcaccgtg aagctccccg aagcgtagcc ggcctccgcc aggagttttt tggccagggt
     2521 tgagtccttc ttgatctccg gctgcggcgt aaaggaccag acggccgagg gcagggggct
     2581 cagcggtttc gccgccctcc ccttccacac ccctttaata aaggcgtcat agtcaaaggc
     2641 atgagccatg gctttgcgta ccctcacgtc cttgaaaggg ccgtcctcgg tcatgtacag
     2701 gtggatcaga cgggggaccc ggtgctgctc gaccaccact ccgggaacct tagccacctc
     2761 atccagatca tccagttcga tcttctcgga taagtccacg tcgcctttgg tcaggagcag
     2821 gcgatgggtg gcggcttccg gaacataccg cagaataatc cgctgggcat gcttgccccc
     2881 ccagcctccc cagtatgcgg gattctgttt gaaactgaca tactgcccct taacccattt
     2941 ctccagcaga taaggccccg tcccgaccat gttctcaacg caccaggcct ggccccagtc
     3001 attgttcttc tcgttggcct tggcggctga agggctgatc atgtacttct tcaccaaggt
     3061 ttcaatgaac ggcgcaaaag gcttcttaag ggtaatgcgc agggtcaggt cgtccaccgc
     3121 ctcgatgccg tccttctcgg gccataccgt ctggggaatc cgggtggccg gcaccttgtt
     3181 cagggcgaac aggcgttcaa aattccactt ggccgcttgc gcgttccagg gagtattgtc
     3241 atgaaatttg attcccttcc ggagctgaaa ggtccagacc ctggcgtcgg gagagccctt
     3301 ccaggactcg gccagtatgc ctacgatctt caccgagcgg tcgggcataa tcgtgtactc
     3361 catcaggggt tccagggagg cccgctgggg tttctggtcg tagtcgtagt caaactggtg
     3421 ggggtcaagc ccacgggttt cggtcttcac cgcccaaacc atggggcggc tggaagcctt
     3481 ggcggctgcc tgctcggctc cttgagcctt gctccaaccg aacatctgcc atcccgcgaa
     3541 aaaagcaact ccaaccagga tcaagatgaa tatgccgatg cttcgcttta ccattacgct
     3601 catcctcctt tcgctttttg atatggaaat ttattcatac tcttttgagt gacggcccta
     3661 ggccagcagg gtaattttaa aaaccaccgg atttttcgaa tccgagcagg agatgcgcgc
     3721 cacgcccggc tcggattccc aggggaaccg cccgccgtat ttcagggtaa acagggcggg
     3781 agtcatctca tggaacgccc aggcgcagaa gttggggaaa gtctgcggca cgcctttgtc
     3841 ttcgaacaca aaggtatcgc cggccttaaa cccgaagggg cagacgcccc gctcaaggat
     3901 cttttcgatc tccactttaa atttagacat gatcctctcc gcgttcagtt ctttaagcgg
     3961 gcttgcccgc cagaatcgat tcaatgggcc ggtagggaat gtcgaagcca aagtaggaag
     4021 gccccaacac cttgaggccg gccggctgac gatagaattc caccgcgcgc agccccagca
     4081 cggccagccg atccccggaa tcgattttgt tgttggtggg gggcaggccc gtctccaggt
     4141 tgatcaccga gagaatatcc gggcaggtga catagggttt tccgttgagc caggaaatca
     4201 tgttctcgtt ctggaaccag accttgaaag actggccgcg aaaatcttcg atccccttca
     4261 tctcgtactg cccgatcata tacccctgtt tgctttccca ggttttcttt tccaccaccc
     4321 cctggaatag cacccaaccg tccagctccc gggccacgaa ggcggccatt tcctttcccc
     4381 ggcctttggc ctcgcgcagc agcctcccga tccgcaggca ccgggaaatg ctgccgcggt
     4441 tgatcaattt cttcatttcg gttcccttga gcaggtgacc ggctccgccg atgaggccga
     4501 cggtggtcat gctcaggtgc ttctggatgc gctccatcat ggcgtggctg gaggcctgct
     4561 tgatcaccgt ggtattgccg gcaaaatcgc agaacgcccg cggccagatt tccttcccgg
     4621 cgatggagat cttcaaatga acgctcgccg ggatggctcg tcctgcgtaa tccccgtcca
     4681 ccgccgggat ccccagggcg gccgcggcgc tgatgggagc gggcgtgttg agccctccca
     4741 attcgcaggc ggcaatggcg gaaacccgga tgcccacgta ggattcgagt tctttcaccg
     4801 cctcgacgat ctcatggtcg acccgttttt caagacccag ccgttctttc aacgccagca
     4861 tctcctccga agggggggcg atggaaccga cataggcccc cgtgcagacc caggcgtcat
     4921 cgggaagatc ctggggatcg gtgagcctaa tttcccggcc tttatccaga gcttggcgca
     4981 acaaatccag tcctatgtcc ggacctcctc ctcccccggt tcccatgaaa cagcagccat
     5041 atacgagatc ctgcgcttct tctaccgagc gaattttcca gtcagccatg ttttcctcct
     5101 ccatttcgag aaccgaaacc catccgggga acggtcgtag cggaatcgat cgaatcccac
     5161 ctacccgcca gccatcactg gaaaaactgc taggacgtct ccgtcttcca gcggggtgtc
     5221 gtagccctgt aggttttgaa tcggacggcc gttcaaaatc agcagcaaat cgtcttgagc
     5281 cacctgctgc agaggcatgg gagaattttc tctgctcaga tttttaaagt tttccttctt
     5341 cacctgagcc atgagatccc gcacacaggc gcctagcttc atttgagcgc tgatccgctc
     5401 agggaacgcc ccggtgaaga gtttcatgtg aacggtgatg ggtcgcgtcg tttttttcat
     5461 cgatgattgc gcccttgccc ctgtaattcc tcctacaatc aatccttggc cagctttccc
     5521 atccgatcca gctccgcggc aacatcccct agatcgagat cgcccagggt cttgcaccgc
     5581 ggaagcccgg tggtcttttc ccagccgtgg agctcatagt actcatccag catgcggtcc
     5641 aggtaagccc gggagttgat cgactctctt accggcgagg gacgatcggg ggccgccttc
     5701 gatcgatcgt cgtctttggc cgatgtctgc ggcaggactt cgttcatcaa acgatggggc
     5761 agacagtcat gctcgcgggt ggccccctcc cgcacattga aggccttctc caggttcacg
     5821 atcctacggc cggccagcag gaggctttcc gattcaaaaa aatcaatacc ggtcgccgca
     5881 gacagcatac gggccaggag ctccggcgtc accccatacc gagcggtcgt ggcaaaagag
     5941 cacaatccga ccgcatccgt ggcggcatag cagtcttcgt gccagcggac gatatcggcg
     6001 cgcttctcca gcgtgcgcgg aaaggcatac ctttcgtgcc cggtgatctt ggccaccagc
     6061 gcccgcccgc cggggcgggt gccgaattcg gccaggacct gggagtgaag atggtcggga
     6121 ccgcggggat tgacggcaaa ggccagggca tacccaaaag cagttctcgt ttccacccgg
     6181 gattgttcca ggcccttggc ttccacggcc catttctccg agcctttccc cacgctcttc
     6241 gccgctcggc ggaccccgcc gcccagcctg gcgccaaacc ctttatgtag ggcaacctgc
     6301 tccaggaggc ttaagatcgc ctctcggctc ccccactcca gcttgacgcc gccggtatct
     6361 ttctcggtaa taacccctct ttcatagctt tccatggccc attggaccac gctcccggcg
     6421 gaaatcgtgt ccatcccgta gaggttgcaa agctcgttgg ccttgatgac caattctacg
     6481 tccacggtcc cgcaaccggc ccccagggcg ctcatggtct catactccgg acctcccccg
     6541 tacgctcccg tgtagggtcc cgaggcgacc tcgcaatacc ggtggcagct gaagatgcag
     6601 gcgttgcagc ccatgcgccg cttcagatat cccgcctcca ccagataggg gccactgatt
     6661 ttttccgcat tatcgatccg tccgctccgg aaattaaaac ttgggaagga acccagctcg
     6721 ttgatcactg cgacgctggc ggaagttccg taaagatacg aggttttggc gctgaaatcc
     6781 tggtaggaga cttcaatgga ctccatgatg gctttgcgga attcctgcgg ccgcgccaca
     6841 cggattttcc catggccgcg gaccgcgacg gccttcagat tcttggaacc catgaccgcc
     6901 ccgactccgc cgcgggccgc cgcgttgtaa taattcatca tgatggaggc aaagagtacc
     6961 cggttctccc cagctggtcc gatgcaggcc acctggacct ccggatcacc caaggcgtgc
     7021 tcgatcgccc gggtggtttc gcggacatcc ttgccccaca gctccgccgc gtcgcggatt
     7081 ttcaccgccc catccaggat gaccaggtaa acgggcttcg ccgcccggcc ctcaatgacc
     7141 aggtagtcat agccggcgaa tttcagctcg gcaccccagt gcccgcccac gctggtcttc
     7201 aggtaaagcc cggtcgccgg ccctttgcag cttacggtgg tgcgtcccga cgtgggggcg
     7261 gaggttccgg tcagggtgcc ggttccgaaa atcaggaggt tttccggtcc caaagggtcc
     7321 acacccggcg gtaaaagatc ccagagaagg cgggcattga atccccgggc gcccagatat
     7381 ttcaacacct tcttctcatc cagaggcttg ggggtgattt ttccagtggt caaattgacg
     7441 tgtaacactt ttccggcaaa agcgaacggc atctttccct ccttgcttca tccgacaaag
     7501 ggacgctcgg gcgttccgag ggccgaatct tcattcccaa aggtgtagcc agaaccccgg
     7561 cgaccgcagc ctcagctcct cagcgccaga ccagcgcttc cggaagacgg cagaccttga
     7621 cgcattgagg gtctcccccg caaaaatcgc acttgaccgc tactcctctc ccctcctgca
     7681 gatggatcgc ctggtagggg caggcatcca cacaggcgcc gcagccggtg cacgcatcct
     7741 cattcagaat cacccactgg ttcttgggct cccgccggat ggcctctacc ggacaggccg
     7801 ggatgcaggg ggcctcctcg cagttgcggc aaaccagggg actgctctgg ccgcgggatt
     7861 cgttcttggc caccctcacg cgggcttcgc cgtagtcgaa ggcgtctgta tgggaaaagg
     7921 aacaaatcaa catacagctc ttgcatccgg tgcagttttc gggccgaaca acgagcttcg
     7981 ggggcatagt ccctcctagt aattatggtc cacccggaga ggcggaagca ttcaggtagt
     8041 cctttaagtc atactgcagt ccgcggccgg gggcgaggac ctttccggcg aggacctccc
     8101 ccaggaaaat ggtgtggtcc ccggagtcca tctgattgat taccctgcag tccaggtagg
     8161 ccaggcaatc cttcagtatg ggcgctcctg tcttttcggt gaaataaggc accttgcgaa
     8221 atttatccac atcccggcct gactgttttc caaagtgaat cgccagcctc tgttgcccgt
     8281 cccgcaaata atttacggca aagatcttgc tggtttttat gagatcatgg gaatagtgct
     8341 ctttgtagat actggccatg accaaaaacg gctgctccga ggcgcggctg acccatgccg
     8401 ctgacatgcc gttcagcttt tggcccgatt tcgtagtgac cacgaccacg caccggacct
     8461 ggacgttgaa ggcccggtca atatccgtta ccggtcttcc catccttgtg gtttccgtct
     8521 cctcggccat ccttatgtgc tcctttccga aaaacaattt atccgccgga aagcttcccc
     8581 agcacaaatc catagttccg gtccgctgcc aggatcccct tctggattta caggcccgcc
     8641 ttcacgatga gagcaccctc caaggccctc cggaccacct gctgggagct ttttactgca
     8701 ctccattacg gccggcgata aaaatccttc ttgatccgca aaagatggcg cttcatcgca
     8761 tcgcgagcct gatcggcctt gcggttcttt atgctctcga atattttttc atgctccagt
     8821 tgcatatccc tcagtctcgg attgtgacgg taggcggtca cctcgatcgc ttcgtaaagg
     8881 atctgttctt tgagagacat aagggtttcc accatggact gcaggatgac attcgatgcg
     8941 gcgcgggcga tgcccagatg aaaggaattg tcttcccggc tggtgaaggt gcctttactg
     9001 attttttccc tcattttttc gagcacctgg gcaagatcgt ccaggtcttt ttcgctgcgt
     9061 tccctcgctg ccagccggca gatctcgggc tcgattaaaa gacgggcccc aaccagttca
     9121 taaggactga cacttttccc gttgcgggac aagagaacag aaggctggat agggctgacg
     9181 gatttcacat aataaccgcc gccctggacg ctctccaaaa ttcccacgta atccagcaca
     9241 ctaagggctt cccgcagggt cggccgggcg attttcagct gcttgaccag atatccctcg
     9301 gtgggcagcc tatcgcccgg tttcaggtcg tagcgctgaa tcgattgcca gatttcttcc
     9361 aggatttccc ggtagatctt cttcttcccg attggcctca gctggaattc catatccctg
     9421 cccctggagg ttagcggtgg tccggaatgc agactttatt cctgcggttc ggcttctgat
     9481 tgcggataat aaaacttgtc tgacaattta cgcgggaggg ctccctttgt caagaaaaaa
     9541 ttggacagaa aaatttgcac ggaaaacttg ggcgtaagcg gacaggatgg atatcattct
     9601 cagccctggg caggaggcga ggatctttcc gttcaaatgg gtgaaagccc gcgtgctgat
     9661 cacgacttcg gattccttga gcctctcgat gagctcatcc tccgtttccg ccttgctgcg
     9721 gtagattttt aattcaccca gccatttgat ttttttctct gcccgcgctg cctgtaacca
     9781 ccggtggaag atcatccggg atgatgattc gaagattcat tctatttcac tcgatcgctt
     9841 tcttaccaat catggccgac tggataccca ctaatcaaat atccttgcca ggtcttcttg
     9901 tcaaggaatc caaaaaggat tccgccgctt tcagccatag aatttagtcg ctgtcgggag
     9961 tttgggcaag agaaagcagg tgagagataa gggcaggatc aacgatatcg aggcaaatgt
    10021 aaaaagagga cggaagggag caaacctgga aatcgaatcg aattcaagat gtctaaggaa
    10081 atgctgttgg gttgtcatgc tgcggcgtcg ccgttgactg cgaaaccgca gaaccgcaaa
    10141 accgcagcac ggtggtccct atctcttcgg agctgttcct ctttatttcg tgtaggtata
    10201 ttcatccccc gggtttagcg ctaagacctc gacctttggc gcttccttct tggccaaggc
    10261 aatgaactcc ttggggtcct ggacgaggat gggaaaggtc ttgtagtgaa tggggatgac
    10321 cttcttgggt gagatcattt tggttgcctt ggcagcctga aatgggtcca tggtgaacac
    10381 gctgccgatg ggcagcattg ccacgtcgat tcgatatacc tgcccgaaga gcttcatcga
    10441 gtcgaagatt cccgtatccc cggagtggta gagggttgta ccatcctcca acttgatgac
    10501 gtacccgcag ggggcaccgc tgagagtgga atgaaaagcc tgggtcatgg taacggaaat
    10561 gccctcgtaa accaggttcc cgccgatatt catcccgtac ccgaaatagc agactttttc
    10621 cgctggaata tgaaactcgc tctggatgcg gcggcccgtt tccacgttgg ccacaagcaa
    10681 gcccccggtc ttcttgacaa tatccggggc ttgccctacg tggtcgaaat ggtcatgagt
    10741 caccagaatt agggttgcct cttttacgtc gtccaggcta aaacctccga ttggattttc
    10801 aaaccagggg tcgatgtaga tgaccttacc cccgctggaa gtgatctgaa aaccggcatg
    10861 agataaccat ttgatttttg tcttgcccat aaggagcctc cttgaaatta ggtaataggt
    10921 gataggtcat gggtcatgga aattataaat ggtgcaaagc gaaggtgcaa ggagaaaggc
    10981 ctctgacttc gggcctgttt cttcatcctc cgaggtatgc tttcttgaca tgctcgttgt
    11041 cgatcaagtc ggaagccggg ccttgtaggg cgatggaccc ggtttccaaa acatagcctt
    11101 tgcgggcgat gcgaagggcc atgtgggcat tttgttccac caggatgatt cccacctgcc
    11161 gttggttgat ctcgccgatg atccggccta cctccttgac catgagcgga gcgagcccca
    11221 gcgacggttc atctaggagg aggagggtgg gttgggacat gagcgcccgg ccgatggcca
    11281 gcatctgctg ctctcctccg ctcaggcttc cggcccgctg gctccgccgt tcccgcaaaa
    11341 tgtggaaatg ctcaaaaact ccttccagat tcttcttgat ttcttccttg tctttcctcc
    11401 gaaaagcccc catattcagg ttttcccgga cggtcatgaa ggggaaaacc cttcttccct
    11461 ccggcacatg ggcgattccc cctcgaacta catcttgagg cgaaaggctg tcgattcttt
    11521 ttccctggaa aacgatttcg cccgaagcag gatgaaccaa cccggagatg gcccggaggg
    11581 aggtgctttt ccctgctcca ttggccccga tcaaggttac gatcgcccct tgatcgacct
    11641 ccagggagat ccctttcagg gcttccaccc tcccataaaa gaccctcagg ttgcggatgc
    11701 caaaccaagt ttcgccgctc acgttaccgt ttcacttccc aggtaagctt cgatgacctg
    11761 ctggtttccc cgcacttctt ggggggttcc ttgggcgatt tttcgcccgt aattgaggac
    11821 cacgatgcgg tgactgagcc ccatgatagc cttcatatcg tgctccacca ccatcaggct
    11881 gatgccccgc tccctgatcc cccggatcaa ttcaatgaac cttcgagact cctccgggtt
    11941 catgccggtc agcggctcgt ccaagagcat aagcttaggc cgggccgcca gggcgatggc
    12001 cactcccaga gcccgctggt ggccatgggg aagattatgg gccatttcat ccttcaaatc
    12061 cgtcaggttc atgtactcta ggatttccag actccccctc cgggctgctc cctcgtcggc
    12121 cctggcctga acactgttcc ccaaaagtcc ccagaatccc gattgcacct gtaaatgatg
    12181 ggcgatgacc aagttttcca gaaccgtttt gtgctgaaag agattggtgc tctgaaaagt
    12241 ccgaaccaag ccttttctgg ctacctggtc gggccgaagg ccagtgatgt cgtccccctg
    12301 gaaaagcaca cttccatagg tgggggggaa aaatccggta aggacgttga agagggtggt
    12361 tttcccggct ccattggggc cgatcaaccc caggatttcc ccggagttca ggctgaagct
    12421 cacctcactc agggccgcca gccccccgaa gtgcttgctg atgtctttga tctctagaat
    12481 cataaagaat aaatggggtg tagataaacg cagattaaaa tcattaacac ggactcgtgg
    12541 atcatttttg gtaattccat tttttatctg taggtttctg tgtcctgata gattaatgct
    12601 ctttcttccc gcgcaaacgg gccggcaggg tgaccagtcc acccggcagc aggaacatga
    12661 ccgcaatcag aatcaccgcg taaatgaccg gctccagctc ccgggccacc cgcagcagtt
    12721 ccggaatcag ggtcatgaaa aatgagccca gcggggggcc ccacacgctt cccgcccctc
    12781 ccacaatcac gaataccagg cagtaaaccg actgccagat cgtgaaaaac tccgggctgg
    12841 tgaaattaaa gtaatgagcg taaaagctgc cgctcaggcc tgcgaaaaaa cacccagtgc
    12901 agaaggcgat cactttgtat ttcatgatgt tgatgccgat ggactcggcc agggtgtcgc
    12961 tttcgtggat ggaagaaaag acctgcccga tccggctctt atcaatcctc accatgacca
    13021 gataggtgac cagggacagg gccaggatga gatagtaaaa agggatccgg gttttgaagg
    13081 aaatggagaa gaaggacgag gggggaggaa cattgggaat ccctccatag cctcccagga
    13141 ggctgggcca gtggacgatg agcaggcgga tgatctcggc aaaggcgaag gtgatgatgg
    13201 cgaagtaaac ccctttgata cgcagggtgg ggtacccgat cagcagggca acgagggagg
    13261 agatcaaacc tgctagggga aaggctagcc agaaagagag cccggccttc atggtcagca
    13321 atgtggaggc gtaggccccg acggccatgt aggccgcatg gcccagggtc aattggccgg
    13381 tggacatgat gacccggact ccccacaccg acgtcgtcca gattccggtg gtaatgagaa
    13441 tgttcaaaag gtaggggtcc tgggtgaatg ccggaacggc tatcaggcac agcagaatga
    13501 tcagaaggac gactcttctc ttatccatgg ccgaagagcc cctgcggtcg gaaaagcaga
    13561 acgatcatca cgatgataaa acccaggatc tcggtgatat ccccgaaaaa ggtattccca
    13621 aagctctcca caaaacccag cagaaggccg ccgacaatgg cgccgggaat ggaccccaat
    13681 ccgccgatga ccaccacgac cagggccttg aggacggcgt gcatccccaa aaagggattc
    13741 acatagaaaa gaggagccag gagggcgccg gccgccccgg ccaaggccga actgatcccc
    13801 atgcacaggg taaacgtaga atccacccct attccctgga gcgcggcggc ctcccggtct
    13861 tgggaaaccg cacgcatggc ttggccggtc ttggagaact tgatatatag ggtaagggcg
    13921 gccatcaaga cgatggccac aataataacc gcgacccgtt ccttagacaa ggtggccccc
    13981 cagacctgaa tgactcccga gaaaaccggg gccaccgatt tgtctttttc cccaaagatg
    14041 atcagggcca gccccccgat cagcatggca attcccaggg cgatgaccag gctggggtca
    14101 tgttcgtcgc ggaaaggacg gaaaaaaaag tggtagacca ggaccccaag gactccgatg
    14161 atcaccatgc taagggccat ggcagtgaaa taatggaccc ccagatgctc gcacagcagg
    14221 taggtggcaa aggcccctaa catgtagaac tcgccgtggg cgaagttcag aaggcgcatg
    14281 atcccgtaga tcaggctgaa tccagaggcg atgagcacgt aggtcagccc gagcataagg
    14341 ccgttcacca gcacctggat gaagaaggaa aaggtcatgg ctctttttca aggaagcgcg
    14401 ggcatcatgg ttccccggtt gattagattc cctttgatga tttcggaaat atacaccggg
    14461 gtgacgatct gacgcttgat tccgtagtgt tccttgcccc cgaatttgga ggccccgaag
    14521 atcgtctgcc actcttcggt tttttccatc gtctccatga ccttggtggt atccagagtg
    14581 cccgactttt ctagggcctg gatcaggaca tccagataga cctggtattt gggagtcacc
    14641 ggatcaaagg gctttccgta catggcgatg tagccgtcgt agtatttctt ctgcccctca
    14701 gtcgtgtagg cacccaggag atcatgagtg ttgctgatat gcccctccga gttctgtacc
    14761 ccggcgatct tgcagaattc cgccgggtcc atggtagtgc tggagaagct cttgcccgtg
    14821 taacccagct cccgcatctg cttgaccatc agagcctggt ctcccggcgg cgccccgctg
    14881 aagtccacaa agtccggttt ggcggcaatc attttgttga gcagagggta aaaatccgaa
    14941 gttccccgct cataaaattg agaaaaggcc acctggtact tgttctgccc agccatgcgc
    15001 tggcaatcct tttccaccga ccatccgctg gcatcgttgg gttcgatgaa ggcaacggta
    15061 ttcagattcg cataggtctt tctcatccag gggtacatcc cctggaggat ttcgttggaa
    15121 gtcaagacca tacggaaggt gtagggtttg tcgggggtga ggacctcctt ggcgtaagaa
    15181 ttcaccagga agaggatctt gctgggctcg gtgatgggct gaacggccag gatgaccgca
    15241 gaggattgag agcccaggat gaacttgacc cggtcccggt tgaccagctt ttccgccgcc
    15301 atccggccgg cggcggcatt gaacttgtca tcctcccaga tgatctccag cttgtaagtg
    15361 tccgccccca ccttgaggcc tcccttggcg ttgacctcct tctgtttcac agtaatggcc
    15421 ttctgggcgg ccattcccca ggtcgctccc ctgcccgaga ggggaaaaat ggctccgatc
    15481 ttcagttctt tctgggcggc ataaagggga aaagacggaa tccccaaagc ggaaacgact
    15541 cccagaatca ctgctttgct ccagaagctt ttaagaaatg atttcatcga gcgcctcctt
    15601 ttttcctccc atatccttcc acccgggtac tgaaatgaat ccagccactc cttcccggct
    15661 accctgcata atccgctatt tttccgcggg ggtctcttgt tcgtacttcc gagtcatttc
    15721 ctccacccag cgaacatctt taacttccag gcagctattc tcgccattgc gaatctcggt
    15781 gaagtagaag ggggacagcc cttgatgagg aatcccgtat gtccattggc aggtgaattt
    15841 ataaaccccg tccagttcct cccactccat ccctgccatg gtcttcctga cctgaagcgg
    15901 gtccatgctg tttgccttcc gaattccctg gatcagggcg ttcgaatagg ccctggcctt
    15961 gggggcgtac tcatcccact cgccgaaccg tttcacatat tctttttcat attccagggc
    16021 cgcagacgac ataaacggag gagccatgac attgtagata tggccctcgg cgtttttctt
    16081 gccggccacc gcacaaaatt ctttcaccgg gataagggcc agactcatcg tccttccggt
    16141 atatcccagc tcccgggcct gcttgaccat caaaaagata tccccggccg aagaacttcc
    16201 aaaatcgata aaatccggtt tattagccag gacccgcgtc aggagggggt agaaatcttt
    16261 ggtccccggt tcgtaaaatt cttcggtaag aatttgaaag ccgaaatact tgcaggatcg
    16321 gcggctgtcc ttcacactgt cttttccagt cacatcgctt cggttgatga tggccacctt
    16381 cttgatcagc gggtatcgct cggtgatgat tttgtaatag cagggggcct gttcgactcc
    16441 ggtcaccgag gggcgaaaaa aatccggatg ctccggcccc atgaccgccc gggtgtacgt
    16501 gttcccgcaa acggtgatgt tgttctcttt tgaaatctcc tgccacgcct ggcgggatgc
    16561 cgaactgatg ggccccacga tgaattttac tttatccttg aagatgagct tctccgcggc
    16621 catgcgtcca ctcgcgccgc tgtacttatc atcctcgaag aggaactcga ttttgtagct
    16681 tttcccctcg atgggcagtc ccccggaggc gttgattctt tcctgttcga aggtggcggt
    16741 tcttttccag accgcacccc agggcgcccc cggcccgctg aggggtgcga tcatcccgat
    16801 tttcagagtc tcctgggaag atgattcggc gggaaaaagc ggaagggtga agatcaccgc
    16861 ccaggccaag gtcaaagcca accatttttt cctttccatt tatgccctcc ttgcgagcaa
    16921 gttggttatt cgctaatgcc gcgggcaaaa aaaatggacc tgaaaatcta ggttaaacat
    16981 gaaccccttt ggccctagga cgatattcgt ctgttgcgaa tggaatcgag tgccagatgg
    17041 gaatcaatcg caggggcgat catgcgaagc gcgtgatggt tgcccatctt tcgggcggtc
    17101 atgacgcctc ggggatgaac gcccccacca catcctgttg actccggttt gcccggatta
    17161 gattaattcc atctcctgtt gataaaaatt ctgccaagat tggtactgga gtctgctaat
    17221 cgtgcttttc gcggtggaat gcgggaaagg ttgcctatgt atatccctcc ccgaggtcaa
    17281 tgtcaaatcc tttcttctgc gaacagggaa atcacctacc agcatcggag ttccccgcaa
    17341 gcccatcgaa gaactcccgc agtctggccc tctgaggttc ggggacgaat tcgatattca
    17401 tccgccgttc gctgacctcc caatatttct ggcgggtcac ctgcagcaga tcctccatga
    17461 tccccttcaa aaattccgga cccatgcctt gaaagctccg ccggatgagt gtggcggcct
    17521 cattctggcc ggagtacaga aagtaactgg ccagcagggc ttgggccttt ttcaccccgg
    17581 gcaggactgc tttgccctgc cggtcataaa ggaggaaccg ttcgagaagc tcttcccgga
    17641 tggtagaatc actttgccag gctcgcttca ccaaagcccc taggtcgtgg gccaccgcct
    17701 cgaccacgaa cggcaggttc tgttcccggg cagcggcccc gtagtaggag aagtaatagg
    17761 cgatctcgga aacctcctta ggaaagttcc cttccagggt ttcagcaaaa gtccggtatt
    17821 ggtcgaagat gatgaacacg gaccggacgt cgtgccgatt gagggtcagg cggatgaagg
    17881 tgttgaagta ttcaatggtc agctcgcgaa gagcggggtt ttccagggag gcagcttcca
    17941 ggcccagttt cctcaagctc ttggcgatcg tgctggtcag ctccggcatt cgcgggcagg
    18001 cggccctcaa ggcctcgaaa aactggtagt agagcttcat ctccatccag ctccggctgg
    18061 cggtcatttc atccacggcg agagaggaaa atcccaaaaa gaaattctgg tcggcctcaa
    18121 accaggaggg acgcagttct tctttgacct tccaatagtg acgcgccacc cgttccaggg
    18181 tcagaacccc ttcaatggcg gtattgcggt cgaagcggtc aaccgaacgg accgaaatat
    18241 tcgcaatgtg ctccaaagtt tcggcgactc gctcccgatt cctcccagcc ctgccggaat
    18301 gacaggcact cctcaaactg gatcttacat cttcttccag gaggttgagc agggtccggg
    18361 ggtgcaagaa gcgaaagaca tatatcaaat agggcagaat caagagcagg gaggcaaggg
    18421 tgaggaccat catcaggcct agctgaaacc tcgggaccga ggtctcggtc aaggaatagt
    18481 tggtccacag gccgctgatg ttggcaaaga tgataaatat gagcaccgca acattcaccg
    18541 ggtttcgaat gaacaaatcc agaaatttca gggaatacag attggcggtc agaggaacgg
    18601 ccatcccggt tacgataaag agaatactca tcgcctggtt taggtttcgg ctgagcaaat
    18661 ttaccaggct ccgggcttcg gccggggtga gggaagacag cggggggaga gagatctgtg
    18721 tggaaaggag ctcggcaaac gtctgaacca ggaaaacgcc catggccccc gcgattaggt
    18781 agagaatcaa cctcaggaag cgtagggtat tagccttcat tcctcagcct catttccttc
    18841 gatggggaaa aagcctatca taatttcctc taggctgcaa cctgaagtca tcgaatgcgg
    18901 gcggctccct ggcaggttat aacctgccat tcagtaaagc aatctcggat gcggtcatcc
    18961 tacccttagt ggctccggga ggcgcaggca aactggagca ttttatgaag gagcggtcaa
    19021 gggaggggcc aaactcctgc tggccacgtc cgtcttccac tttcgaacct ttcgggtccg
    19081 ggaagtgaaa gagtatttgc acaataaggg cttgaaagtt aatttttaat cgaaccctgt
    19141 aggggagttc tcaaacccgt tctgaatcgc ttggacaaaa aaatcgagtt ttaaagcatt
    19201 tcgatcaaag cccggcggtt cagtttaccg gttgcgaaca agggcatctt ttcgataaag
    19261 tggacctctc tcaatttttt gtagggagcg acctggccgt tgacgaactc cattaattcc
    19321 ccggcggtga ccttggctcc ttctttcacc gagacgaagg caaccgggat ttcccccgcc
    19381 atatcatcct ttttgccgac cgccgcgcat ccaaggaccg ccggatgttt gtacaaaatt
    19441 tcttcgagca tccgcggggc gatattgtac cctttgtatt tgatgagctc tttctttttt
    19501 ccggtgatgt aaaaaaagcc attttcgtcc atcgtgccca ggtctccggt aaaaagccac
    19561 ccatctttga atgcctcttt cgtatcttca ggctttttcc aatagcccag cgccaccgag
    19621 gggccctttt gaatcaattc tccttcctcc cctgggcgca tttccttcgt tccggtttct
    19681 gtatccacaa ccttcacgtc cgt
//
LOCUS       DASPRR010000003         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_646184, whole genome shotgun sequence.
ACCESSION   DASPRR010000003 DASPRR010000000
VERSION     DASPRR010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646184"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VEL68_00140"
     CDS             <1..192
                     /locus_tag="VEL68_00140"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538555.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXZ34404.1"
                     /translation="LEISSFPQACMNADRMSAYTQWDFPLLEAMRNEFERGIPMVEIE
                     GLSGAARFAGGAGRGGKFE"
     gene            284..1813
                     /locus_tag="VEL68_00145"
     CDS             284..1813
                     /locus_tag="VEL68_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ34405.1"
                     /translation="MPSEVYVAGVGMTKFGKSDLTLPQLMVEAASRAILDARLNTIDY
                     VFIGVMSVEEFTGESNFAALITDRLGLPGIPSSRVETASSTGSAVFESAFYAVASGYH
                     RNVLILAGEKMTHLPTAQTTKILAEVIERNERKYGASMPSLAAMITRRYQYNARLSDK
                     AMQSVLAKVAIKNHYNGSLNPYAQFRKIITAADYFGSRFVSTPLRTFDCAPITDGAVA
                     VVLTLDKRPIRVSGLGHATDTLAVRHRDSLTSFKSTRLAAWQAYNMAKLSPKEVSFAE
                     VHDAFTTFEIIGTEDLGFFPHGEGWKAVEEGVTSIKGRLPINPSGGLKARGHPVGASG
                     LAQIAEVVLQLRGDMETSRQISGARIGLTQNIGGLANNNLVTILERADRYRPIPVYWK
                     PDYDPGNIKAREVPEEPLAVDTGTLETFTTLFTTPEGIPSPLTLGFVSIPDGRLIMAR
                     AAQEDLRIGQTVKVEKEGDLFYIQPSTPQPRPSPEKGMEGVKKWLHRALSSSDPKEDE
                     R"
     gene            1810..>2238
                     /locus_tag="VEL68_00150"
     CDS             1810..>2238
                     /locus_tag="VEL68_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXZ34406.1"
                     /translation="MTEEKQETVGQFLRKEREKRNVSLDAVAKVTRITKENLEALEKD
                     DFQVISAPVFVRGFLRNYATFLGLDPKEVVGRYDSQTDLVPIPEEEEPPPPPPKEEKP
                     IFKTLLFLCILLVGVAFSFYYYKRSSGPASPPPPSAEVAPA"
BASE COUNT          490 a          663 c          616 g          469 t
ORIGIN      
        1 ctggaaatca gttccttccc ccaggcctgc atgaacgccg accgaatgtc ggcctacacc
       61 cagtgggact ttcctttgct ggaggccatg cgcaatgaat tcgagagggg aatcccaatg
      121 gtggaaatcg aaggactgtc gggggcagcc cgttttgccg ggggagcggg tcggggcggg
      181 aaattcgaat aatctgtggg ggcgaaccat ggcattccta caagctcgac aaagccgcgc
      241 caattctgta taatcaggca aatcttaagg attcgagagg cttatgcctt ctgaagtcta
      301 cgtggccggc gtcggcatga ccaaatttgg gaaaagcgat ctgactctcc cccagctcat
      361 ggtggaggct gcctcccggg ctatcctgga tgcccggctc aacacgatcg attacgtctt
      421 cattggagtc atgagcgtag aagaattcac cggggaaagc aatttcgcgg ccctcatcac
      481 cgaccgcctg ggcttgcccg ggataccatc ctcccgggta gagacggcct cctctaccgg
      541 gtccgccgtc tttgaatccg cattctatgc cgtggcttcc gggtaccacc gaaacgtcct
      601 gattctagcc ggggagaaga tgacccatct tcccacggcc cagaccacca agatcctagc
      661 cgaggtgatc gagcgcaacg agagaaagta tggggcttcc atgccctcgc tggcagccat
      721 gatcacccgg agataccaat acaacgccag gctctccgat aaggccatgc agtcggtcct
      781 cgccaaggtg gccattaaga accattacaa cggctccttg aatccctatg cccagttccg
      841 caaaattatc actgcggcgg attacttcgg gtcccgcttt gtttccaccc ccctgcgcac
      901 cttcgactgt gctcccatta ccgatggagc cgttgccgtt gtcctgactc tggacaagag
      961 acccatccgg gtctccggcc tcggccacgc caccgacacc ctggccgtac gccaccggga
     1021 ttctctgact tccttcaaat ctacccggct ggctgcctgg caggcttata atatggccaa
     1081 gctgagcccc aaagaggtct cctttgccga agttcacgat gccttcacca cctttgaaat
     1141 cattggaaca gaagatttgg gctttttccc tcacggggaa ggatggaagg ccgtggagga
     1201 aggagtgacc tccatcaaag gcaggctgcc cattaacccc tcgggcggtc tgaaggcccg
     1261 tgggcatccg gtgggggctt ccggtttggc ccagatcgcc gaagtcgttt tgcagcttcg
     1321 gggagatatg gaaacgagcc ggcagatctc aggggccagg atcggactca cccagaatat
     1381 cgggggtctg gccaacaaca acttggtaac gattcttgag cgggcggatc ggtatcgtcc
     1441 tattccagta tattggaagc cggattacga ccccggaaac atcaaggccc gagaggtccc
     1501 cgaggagcct ttggcggtgg acacgggaac cctggagacg tttaccactc tcttcactac
     1561 tcctgaggga attccttctc ctctcacctt gggatttgtg agcattcccg atggacggct
     1621 catcatggcc cgggccgctc aggaggattt gaggatcggg cagacggtga aggtggaaaa
     1681 ggaaggagac cttttctaca tccagccgag caccccccag ccgcgtcctt cgccggaaaa
     1741 gggcatggag ggggtgaaga agtggctgca ccgggctctc tcctcctcgg acccaaagga
     1801 ggatgaacgt tgaccgaaga aaagcaagag accgtcggcc agtttctccg gaaggagagg
     1861 gagaaaagaa acgtatccct ggacgcggtg gcaaaagtca cccggatcac caaggaaaac
     1921 ctggaggccc tggaaaagga tgatttccag gtcatttctg cacccgtttt tgtccgggga
     1981 tttttgcgca actatgccac tttcctgggc ctcgatccca aagaggtcgt cggccgttat
     2041 gattctcaaa ctgatttagt ccccattccc gaagaagagg aacctcctcc tccgcccccc
     2101 aaggaggaaa agccgatctt taaaacccta ttgttccttt gcatcctcct ggtcggggtt
     2161 gcctttagct tctactacta taaaagatct tctggccccg catcgccccc acccccctct
     2221 gcggaagttg cccctgcg
//
LOCUS       DASPRR010000004         6976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1129331, whole genome shotgun sequence.
ACCESSION   DASPRR010000004 DASPRR010000000
VERSION     DASPRR010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129331"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="VEL68_00155"
     CDS             <1..143
                     /locus_tag="VEL68_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXZ34407.1"
                     /translation="KERVRNVEDVLKEGDQVLVKVIGIDERGKIKLSRKEALGHPWPE
                     NK"
     gene            237..1493
                     /locus_tag="VEL68_00160"
     CDS             237..1493
                     /locus_tag="VEL68_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ34408.1"
                     /translation="MITKTTLENGIQVITEEIPSVHSVSAGIWVHAGSRDENWEENGI
                     SHFIEHMLFKGTKRRSARQIAKEIDSVGGVLNAFTSKEFSSFYAKVLAEHLPIALDLL
                     FDLYLNSTFSAEEMERERQVIVQEINMVEDTPDEYIHDLFNESFWPRHPLGFPILGRL
                     ETIEGLSRNRIKNFFAENYLRIQPIFAAAGKLKHEELLMPVQEALGRIRLRPKENHRR
                     PPKAHPQVYVKNKPLEQVHLVLGSQGFSATHRRRYAFSVLNTIFGGGMSSRLFQEIRE
                     NRGLAYSVYSFLSSFKDSGVIGVYVGTGENTFSSVLKIIRREMRKLAEDSLRSKELRS
                     AKEQLKGNLLLSLESTDSRMGRLAKSEIYFRRFVSTEEIIEGIESVSAEEVAELAQQV
                     FERTPQSLTILGPVTEKDIPRGLLEP"
     gene            1490..1948
                     /gene="dut"
                     /locus_tag="VEL68_00165"
     CDS             1490..1948
                     /gene="dut"
                     /locus_tag="VEL68_00165"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXZ34409.1"
                     /translation="MNGARVKVQVKRLRESSGEQVPLPRYMTENSAGMDLFADVEGEV
                     VLKPGERRLIPTGIALSIPPGFEGQVRPRSGLAMREGISLVNTPGTIDADYRGEVGVL
                     LINLGQQPFRLKRGDRIAQLVIAPVCRAELELTEELEATSRNEGGFGHTG"
     gene            2180..2491
                     /locus_tag="VEL68_00170"
     CDS             2180..2491
                     /locus_tag="VEL68_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HXZ34410.1"
                     /translation="MPIYEYKCDRCQKIVEVMQKFTDKPLSRCPSCGGRVSRLISNCT
                     FQLKGSGWYVTDYKKGDSGWKDKGKDEKKTETPKTETKESKAEAPKSEKKESKAEAKT
                     A"
     gene            complement(2493..3110)
                     /locus_tag="VEL68_00175"
     CDS             complement(2493..3110)
                     /locus_tag="VEL68_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXZ34411.1"
                     /translation="MIAWIIEILAKFIIDTISSSGYLGIVILMGIESACIPLPSEIIM
                     PFSGYLVFRGDFELFRTGLAGAFGCVVGSVPAYYLGLYGGRPLVEKYGKYVLISHRDL
                     DLADRWFERYGDWAIFFSRLLPVVRTFISFPAGVARMNFTRFVTYTLIGSFPWCLGLA
                     YIGMKLGENWDTLGVYFHEFDIVIGILIAMGVLYYLWHHLKGRKG"
     gene            complement(3112..3939)
                     /gene="uppP"
                     /locus_tag="VEL68_00180"
     CDS             complement(3112..3939)
                     /gene="uppP"
                     /locus_tag="VEL68_00180"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HXZ34412.1"
                     /translation="MDPSSPPFQALLLGILQGLTEFLPISSSAHLILFPWFFQWNNPL
                     LDSLPFDVALHAGTLLAILWYFWRDWLDLIGGFFRILIRRRAQDFQERLILYIILATI
                     PAGVAGFLLEKTIESTFRSPALITLPLILISLLMVYAEKKSRLSHPFERMRLKDALAI
                     GFAQAVALLPGVSRSGITITAGLLQGYRREAATRFSFLLSTPAIGGATLLQARHLLSA
                     GPDEWVLIAIGFFSSAVVGYLAIAFLMRYLRVHTLNLFAGYRLILAAMVIFWVFWKG"
     gene            4219..4653
                     /gene="trxC"
                     /locus_tag="VEL68_00185"
     CDS             4219..4653
                     /gene="trxC"
                     /locus_tag="VEL68_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HXZ34413.1"
                     /translation="MQETVEIVCPKCSAVNRLPSDRLSDHPKCGKCKEDLFAAQPVEL
                     TAETFEKTISRTGIPVVVDFWASWCGPCKMMAPVYQQAAARLEPRVRLAKVNSETEQM
                     LASQFRIQSIPTLVIFRNGREVARQPGAMDLNNLLRWIQTYA"
     gene            4796..5440
                     /locus_tag="VEL68_00190"
     CDS             4796..5440
                     /locus_tag="VEL68_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34414.1"
                     /translation="MAGLPERQCSLPPKTLGGLDKAVSVAFHLSDGILEDIVDGPTKL
                     YFFHYQRANMLLDEMGLKIMNFLQARGWDALPIPASQIIDWEKQWAHVSHKHIAARAG
                     IGWIGRNNLLVSSQFGSRQRLITVLTNMPLKPDKPLPWGCGECRACISSCPSVSIKEK
                     PEDFDHVGCFQMMKVLVKKAGISQNICGLCLKACRGKKIAISDSGVSSQPKTIL"
     gene            complement(5475..6665)
                     /locus_tag="VEL68_00195"
     CDS             complement(5475..6665)
                     /locus_tag="VEL68_00195"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ34415.1"
                     /translation="MAGPLAGIRVLDLTRALAGPYCTMMLGDMGAEVIKVESPDGGDD
                     SRAWAPPFIGKESAYYLSCNKNKKSMTLNLRSDAAKKILTDLIKASDVVVENFRPGVM
                     KKMGFPYENLRAINPKVIFCSITGFGTKGPDAQKPGFDLIAQGMSGFMSFTGEVGGGP
                     IKVGVALADINSGMFAAYGILTALYHREKTGEGQVVETSLFETMVAELTFQAGRYFAT
                     GVPPKPEGNRHPLIAPYESFHCQDGFINIAAGNDNLFANTCEAIGLPELVKDPRFLNN
                     GIRVKNREALTALMEEKMKILKVKDLQKKLDERGVPNGPIWSLDQTLTSEQANALEMV
                     KEVNHPTCGKIKVTGIPVKLSRTPGAVELPPPTLGQHTEEVLTKVLGYSKGEVEKLRK
                     EKAI"
     gene            complement(6727..>6976)
                     /locus_tag="VEL68_00200"
     CDS             complement(6727..>6976)
                     /locus_tag="VEL68_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ34416.1"
                     /translation="EWLAFLEPAGIMCAPVNNIAQVVTDPHILEREMVVEVEHPRAGK
                     FKVTGTPMKFSRTPCKIEKACPDVGQDTEEILKNLGML"
BASE COUNT         1711 a         1814 c         1940 g         1511 t
ORIGIN      
        1 ccaaagaaag ggtccgcaac gtggaggatg tgctgaaaga aggagaccag gtcctggtca
       61 aagtcatcgg aatcgacgaa cggggaaaaa tcaagctcag ccggaaagag gccctaggcc
      121 atccttggcc ggagaacaaa taagctttca gctatcagcg gtcagctttc agccgaaaaa
      181 tcttgccttt gtattagctg atcgctgatt gctgattgct gaaagctgtt tttcttatga
      241 tcactaaaac gaccctggaa aacgggattc aggtcattac cgaggagatt cccagcgtcc
      301 attccgtatc ggcgggaatc tgggtccatg cgggctcgcg tgatgagaac tgggaagaga
      361 acgggatctc ccacttcatc gaacacatgc tcttcaaggg aacaaagcgc cgctctgccc
      421 gccagattgc caaggaaatc gactccgtcg ggggagttct caacgccttc accagcaagg
      481 aattctccag cttctacgcc aaggtactgg ccgaacacct acccatcgcc ctggaccttc
      541 tcttcgatct ctacctgaat tccaccttct ccgccgagga gatggaaaga gaacggcagg
      601 tgatcgtcca ggagatcaac atggtcgagg atactcccga tgaatacatc cacgacctct
      661 tcaacgaatc cttctggccc cgtcaccccc tgggctttcc catcctgggc cggctggaaa
      721 cgatcgaagg cctgagccgg aaccggatca aaaatttctt cgcggaaaat taccttcgga
      781 ttcagccgat ctttgcggct gcgggaaagc tgaaacatga agaactgttg atgcccgtcc
      841 aggaggcttt gggaagaatt cgcctccgcc ccaaggagaa ccatcgtcgg cctccgaagg
      901 cgcatcctca agtctatgtg aaaaataagc ctcttgagca ggttcacctg gttctcgggt
      961 ctcagggatt ttccgctacc catcgacggc ggtatgcctt ttccgtccta aacaccattt
     1021 tcgggggcgg gatgagctcc cggctctttc aggagattcg cgaaaaccgg ggcctggcct
     1081 attcggtcta ttccttcctt tcttccttca aagacagcgg ggtgatcggg gtgtatgtgg
     1141 gaaccggcga aaatactttc tcttccgtcc tgaagatcat tcgcagggag atgaggaagc
     1201 tggcggagga ttctctgagg tccaaggagc tccggtccgc gaaagagcag ctgaagggaa
     1261 acctcctcct aagcctggag agcacggaca gccgcatggg ccgcctggct aagagcgaga
     1321 tctatttccg ccggttcgtt tctaccgaag agatcatcga gggaatcgag agcgtctccg
     1381 cagaggaagt ggcggaactg gcccaacagg tgttcgagcg gaccccccag tccctcacca
     1441 tcctggggcc ggtgacggaa aaagacattc cccgggggct tctggaaccg tgaacggagc
     1501 gcgggtcaag gtacaggtga agcgactgag ggagagttcc ggggagcaag tccctcttcc
     1561 ccggtacatg acggagaatt ccgcaggaat ggacctcttt gccgatgtgg agggcgaagt
     1621 ggttcttaaa ccgggggaaa ggaggctcat tcccacgggg atcgcgctgt ccatcccccc
     1681 cggattcgaa ggccaggtaa ggccaagaag cgggctggcc atgcgggagg ggatttcgct
     1741 ggtcaatacc cccgggacca tcgacgccga ttaccgtggg gaagtgggag tcctgctgat
     1801 caacctcggg caacagcctt ttcggttaaa gcggggggac cggattgccc agctggtgat
     1861 cgcccctgtg tgccgggcgg aattggaatt gacggaagaa ctggaggcca cttcgcgcaa
     1921 cgaaggaggg tttgggcata ccggataaca aaatccgctg tttgaagcgc gaaatccgaa
     1981 gcaataataa aactcaaggt ggcaaatctt cgtggacgat ctttcgtttt tgagttttga
     2041 atttaaggag ttcggatttc gaaatcgtat ctgcattcca aatgattcct ttccttgaca
     2101 aatttcctca gggtgcttat tcttaagttt atggaagtaa atgttttttc ggtggtggga
     2161 gtgatgcgga ggtcatgaga tgccgattta cgagtataag tgtgacaggt gccagaagat
     2221 cgtcgaagtg atgcagaagt ttacggataa acccctttcc cggtgccctt cctgcggggg
     2281 gagagtttcc cggcttatat ccaactgcac ttttcagctt aaggggtccg gctggtatgt
     2341 gaccgattat aaaaagggag attcgggttg gaaagataag ggaaaagacg agaaaaagac
     2401 cgagacccca aagaccgaga ccaaagaaag caaggccgaa gccccaaaga gcgagaagaa
     2461 agagagtaag gccgaagcca agacggcgta agttacccct tcctgccttt cagatgatgc
     2521 cagaggtaat ataaaacgcc catcgcgatc agaataccga ttacgatgtc aaattcgtgg
     2581 aagtagactc ccagggtatc ccaattctcc cctagcttca ttccaatata ggctaatccc
     2641 aaacaccacg ggaacgaacc gatcaaggtg taggtaacaa atcgggtaaa gttcatccgg
     2701 gcaaccccgg ccgggaagga aatgaaagtc cggaccacgg ggagcaggcg gctgaagaag
     2761 atcgcccagt ctccgtaacg ctcaaaccag cggtcggcta ggtccaaatc ccggtgagaa
     2821 atcaacacat atttcccgta tttttccacc agcggccttc ctccatagag gccgagataa
     2881 taagccggaa cggaacccac gacgcatccg aaagctccgg ccaagcctgt ccggaaaagt
     2941 tcaaaatccc cccggaagac caggtaacca gagaaaggca tgatgatctc cgatgggaga
     3001 gggatacagg cgctctcgat ccccatgaga atcacaatcc ccaggtatcc ggaagatgaa
     3061 atcgtgtcga tgatgaattt ggcgaggatt tcgataatcc aggcaatcat atcatccctt
     3121 ccagaagacc cagaaaatta ccatcgcagc caggatcagc cggtagcctg cgaacagatt
     3181 cagggtgtga actcgcaagt agcgcatgag aaaagcgatg gccaggtacc ctacgaccgc
     3241 ggaggagaaa aagccgatgg cgatcaaaac ccattcatcc ggtcctgcag atagcaagtg
     3301 acgggcttgc aagagagttg ctccgccgat ggccggagtg gataaaagga aagaaaaccg
     3361 ggttgcggct tcccgccggt agccctgcaa taagccggcc gtgatggtaa tgcccgagcg
     3421 ggagacgccg ggaagaaggg ccacggcctg agcgaagccg atggccaggg catctttgag
     3481 tctcatcctt tcaaatggat gggagagacg actcttcttt tcagcgtaaa ccatcaaaag
     3541 actgatgagg atcaaaggga gagtaattag cgccggactg cggaaggtgc tttcgatcgt
     3601 tttttccaaa aggaaacccg ctactccggc aggaatcgtg gccagaataa tataaaggat
     3661 gagtcgctcc tgaaaatcct gagctctcct tcttatcagt attcgaaaaa aacctccgat
     3721 caagtccagc cagtcccgcc agaaatacca gagaatggcc agaagggttc cggcgtggag
     3781 ggcaacatca aagggaaggc tgtccaaaag aggattgttc cactggaaga accagggaaa
     3841 aaggatgagg tgggcggaac tgctgatggg taggaattcc gtcaggccct gcaagatacc
     3901 caaaagaagg gcctgaaaag gaggggaaga cgggtccatg gtttagtgat tctctcccct
     3961 cggttgttta tcggaaatga caggatttct cacctgccgg gtatcctttc cgcaactttt
     4021 cattattttt atcatcaagt ttgaaggatg aaaagtcaag gataatttga tttttggctt
     4081 ctcccggggc atgcagaatt gatagaatag aagctgaaag cgtcaaggag catctatagg
     4141 aaaaggggaa gatacgtttc ttaaaaaacc gcccggcctt ttgttcgtat gttggctttt
     4201 gggggaaagg ggggtgaaat gcaagagacg gtggaaatcg tatgccccaa atgcagcgcc
     4261 gtgaatcggt tgccgagtga ccggctgagc gaccatccca aatgcggcaa atgcaaagaa
     4321 gacctattcg ccgcccagcc ggtggagctc accgcggaga cctttgagaa aaccatctcg
     4381 cgcacgggaa tcccggtggt cgttgatttt tgggcctcct ggtgcgggcc atgcaagatg
     4441 atggccccgg tttaccagca ggccgccgcc cggctcgaac cgagggtccg tctggccaag
     4501 gtgaacagcg agacggaaca aatgctggcg agccaattca gaatccaaag cattcctacc
     4561 ctggtcatct tcagaaatgg aagggaagtg gcccgccagc ccggggccat ggacctgaac
     4621 aacctcttaa ggtggatcca gacctatgct tgatctcaat ccctattctc aaaaagacta
     4681 gggttagaga aaggccagag attggacaga aaacgtgaca gaaaaaaaag atgattacga
     4741 agaactaaag gagtttgccc ttgttcaggg gggttccctt tttggggtag ctagtatggc
     4801 gggtctccct gaacggcaat gctctttgcc tcccaagact ttggggggat tagacaaggc
     4861 ggtttccgtt gcctttcacc tctccgacgg gattctggaa gacatcgtgg acgggcccac
     4921 gaagctctac tttttccatt accagcgggc caatatgctc ctggatgaga tgggtttgaa
     4981 aatcatgaat ttcctccagg cccggggctg ggatgccctc cccattcccg cctctcagat
     5041 catcgactgg gaaaagcagt gggcccatgt ctcccacaaa cacatcgccg ccagagcggg
     5101 gatcggctgg atcggccgga acaacctcct ggtgagctcg caattcggat cgcgccagcg
     5161 gctcattacc gttctcacca acatgccgct caagcctgat aagcccctgc cgtgggggtg
     5221 cggggaatgc cgggcctgca tctcctcctg cccctctgtg tccatcaagg agaagccgga
     5281 ggatttcgat cacgtgggct gctttcagat gatgaaagtg ttggtgaaaa aggcggggat
     5341 cagccagaac atctgcggcc tgtgtctgaa agcctgtcgg ggcaaaaaaa tagctatcag
     5401 cgattcagga gtcagctctc agccaaagac aattctctaa ctgatcgctg aaagccaatg
     5461 gctgaccgct gctttcaaat tgccttttcc tttctcaatt tctcaacttc ccctttggaa
     5521 taccccaata ctttggtgag aacttcctcg gtgtgctgcc ccaaagtggg cggggggagt
     5581 tcgaccgctc cgggggtcct ggagagtttc actgggatgc cggtaacctt gatcttcccg
     5641 caggtggggt ggttgacctc ttttaccatc tccaaagcat tggcttgctc agaggtgagg
     5701 gtctgatcca aagaccaaat ggggccattg ggaactcccc tctcatccaa tttcttctgc
     5761 aggtctttca ctttcaggat cttcattttc tcttccatga gggcagtcag agcctcccgg
     5821 ttcttcaccc ggattccgtt gttcagaaac cgcgggtctt tgactaactc cggaagcccg
     5881 atggcttcgc aggtattggc gaaaaggtta tcgttccccg cggcgatatt gataaagccg
     5941 tcctggcaat ggaagctttc ataaggggca attaatggat ggcggtttcc ctcgggtttg
     6001 ggcggcacgc cagtggcgaa gtacctgcct gcctgaaagg tgagctcggc caccatggtt
     6061 tcgaagagag aggtctccac cacctggccc tcccccgttt tttcccggtg gtaaagggcg
     6121 gtaagaattc cgtaggcagc gaacatgccg gagttgatat cggctagagc cacaccgact
     6181 ttgatcggtc cccctcccac ctctccggta aaggacataa aaccgctcat tccctgggcg
     6241 atcaggtcga acccgggctt ctgagcgtcc ggtcctttgg tgccgaagcc ggtgatggag
     6301 cagaagatca ctttcgggtt gatggccctg aggttttcat aggggaaccc catctttttc
     6361 atcaccccgg ggcggaagtt ttccaccacc acatccgagg ccttgatcag gtcggtgagg
     6421 attttcttgg ctgcatcgga acgcaggttc agagtcatgc ttttcttgtt tttgttacag
     6481 gatagatagt aggcgctttc ttttccgatg aaaggaggcg cccacgcccg ggagtcatcg
     6541 cccccgtccg gagactccac cttgatcact tccgcgccca tatctcccag catcatcgtg
     6601 cagtaaggcc cggccaaagc ccgggtgagg tcgagtacgc gaattcccgc caatggtccc
     6661 gccatgaaaa agctcctttc cagggggttt taagaatctt ttaccatgga gttcagaagg
     6721 ggtcaattaa agcattccca gatttttcag aatctcttcg gtatcctgcc ctacatccgg
     6781 gcaggctttt tcgattttgc agggcgtccg ggagaatttc atcggcgtgc ccgtcacctt
     6841 gaattttccc gcccgagggt gctccacttc cacgaccatt tccctctcaa gaatatgagg
     6901 gtcagtgacg acctgggcga tgttattcac cggtgcgcac atgattccgg ctggctcgag
     6961 gaaagccagc cattcg
//
LOCUS       DASPRR010000005         9135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2097729, whole genome shotgun sequence.
ACCESSION   DASPRR010000005 DASPRR010000000
VERSION     DASPRR010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097729"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..892)
                     /locus_tag="VEL68_00205"
     CDS             complement(53..892)
                     /locus_tag="VEL68_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ34417.1"
                     /translation="MKGRITIICENTVTMKGGIGEHGFSAYVETERGNFLFDTGRGDA
                     LIPNLLTFDKDPLAIQKIFLSHGHQDHTGGLASILGVLGGVDILAHPDIFSVRYNISR
                     KDGKEFRRYAGLKFVRPYLETLGGRFILEKGFKEVTREMFLTGEIPRRTPFEKGDPRL
                     FAEIDGKLIPDPFMDDQSLLIDTRQGLVILLGCAHSGMINILDYVIEKTGRERIYAIL
                     GGTHLDFASPAQMEETVGTLKKYGVERIGVSHCTGPKAASRLLAEFGERFFFGSVGES
                     LEF"
     gene            complement(979..1152)
                     /locus_tag="VEL68_00210"
     CDS             complement(979..1152)
                     /locus_tag="VEL68_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34418.1"
                     /translation="MKQEEIMAKLKEAAREGKIPCAMAFKIAKECSVSTKEIGNLLNQ
                     LKIKITSCQLGCF"
     gene            complement(1307..1819)
                     /locus_tag="VEL68_00215"
     CDS             complement(1307..1819)
                     /locus_tag="VEL68_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXZ34419.1"
                     /translation="MKKMIVGLMVVALLVTAGMAMAQGWGRGMGYGPGPGAEYGPGYG
                     PRGSWAANLNLTAEQTQKLQAMRESFFKENLPLRNEMQIKQLELRTLWAQTTPDEGKI
                     LAKQKEINALRAQMQEKGTKHRLEMRKILTPEQQAQVAAMGLGFGPGGGMKGGFGHGR
                     GMGPGNCPRW"
     gene            2187..3494
                     /gene="ugpB"
                     /locus_tag="VEL68_00220"
     CDS             2187..3494
                     /gene="ugpB"
                     /locus_tag="VEL68_00220"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HXZ34420.1"
                     /translation="MKMAKAWMVFVLLAGALALPLTSWAKTEIVWWHAMGGFLGERVN
                     DLTNKFNASQSEYEIKAINKGSYAEALTAGIAAYRAKTHPQIIQVFEVGTQTMLSSGA
                     IYPVYQLMKDQGIKVDWEDFLSVVKTYYSKGGNLYSLPFNSSTPILYYNKTAFEKAGL
                     NPQVAPKTFEDIEKAAKAVVSKGAAKIGFTVSWPSWTLMENMHTWNDQPFANNNNGIA
                     AMATELKFNGKLGVEVMEILSRWQKEGIFTYSGRAANGDQPTINGDAAISLASTALVG
                     TMTRTSKFAWGTGNLPKLSGYPQGNSIIGGASLWVMKGHNKEQYRGVAKFFEFLGQPE
                     QQAWWHANTGYFPLTRAAIKALPEDHFRKNPNLWTAFAQITGGKTTPNSQGIRLGNFV
                     AIRDTIEGEMENIFSGKKTPKQGLDDAVKKGNQTLKEFASMYK"
     gene            3590..4474
                     /locus_tag="VEL68_00225"
     CDS             3590..4474
                     /locus_tag="VEL68_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXZ34421.1"
                     /translation="MKKSIFNNPILPYLLVAPQLLVVLVFFFWPAFETLHLSVFTISP
                     FGDRQIFVGLDNFIALLTSSEYYRSVYHSFLFSLGVTFSCLTLALFLAVLVNQKIRGL
                     AFYRSAILWTFGMAPPISGVIWLFIFHPTYGILNYLLSQVTSYEFNWLLKDWLALVLV
                     IFAASYAHLGYNVAFFLAALQGIPNAVVEAARVDGAGGLRLFRSITFPLLSPVTFFLF
                     IMNMVFSFFETFGIIHAVTQGGPGDATSIMVYKTYVDGFVHMRMGLSAAQSFILMALV
                     IFLTVLQFRYTEKKVSYS"
     gene            4471..5322
                     /gene="ugpE"
                     /locus_tag="VEL68_00230"
     CDS             4471..5322
                     /gene="ugpE"
                     /locus_tag="VEL68_00230"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HXZ34422.1"
                     /translation="MNPSFPASKKARLDGSKVVVHLLLLVAVLLICFPLYYAFVLSTQ
                     SMEEVMVTPQKLWPSSYLWENYVKIWDRVHMGQLLFNSAVVALGVSLGKIAISILSAF
                     AIVYFNFRGKQIFFWMIFVTLMLPVPVRIISTYQVVSELGWVDTYMGLVIPLIASATA
                     TFLFRQFYLTIPDELVDAAKIDGSGPLRFLWSVLLPNSGPSMAALFVVMFIYGWNQYL
                     WPLMASSSDKMKVVVVGIAATIPSGTQPPEWNLVMAVAMMALIPPVFIVLLMQRYFMH
                     GLLETEK"
     gene            5396..6193
                     /locus_tag="VEL68_00235"
     CDS             5396..6193
                     /locus_tag="VEL68_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXZ34423.1"
                     /translation="MSCALKHTEEKRVSYWRGRFPVLVVAHRGFSGAAPENTLAAFRQ
                     AIEIGSDMIELDIQLSKDGRIVVMHDETLERTTNGRGKVVDHNLKELKKLDAGSCFGP
                     EFSGERIPTLQEVLDLAKGRVLVNIEIKNPTHGQYPITDLADKSFQAVKKAGMLDRVI
                     FSSFNPVSLEHIQKIESRAWVALLFHRPWNSFLEMTRGKEYEVLNLRNIHLTKEKISQ
                     VHKEGMKVNVYTVNTEEELEQFVRWGADGIITNYPDRLIRILKATSP"
     gene            complement(6256..6705)
                     /locus_tag="VEL68_00240"
     CDS             complement(6256..6705)
                     /locus_tag="VEL68_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXZ34424.1"
                     /translation="MKTVQDLLKAKKSQEIWSVTPDTILYRALQLLSEKEIGALLVME
                     KEKLVGIVSERDYARKVILEGRSSKETHTKEIMTGKLYVVNPETTMEECMALMTEKRI
                     RHIPVVEKKTNRVLGVISIGDVLKAIISEQKILIENLSSYIMGGKYA"
     gene            complement(6864..7445)
                     /gene="yihA"
                     /locus_tag="VEL68_00245"
     CDS             complement(6864..7445)
                     /gene="yihA"
                     /locus_tag="VEL68_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXZ34425.1"
                     /translation="MKVLSAEFIKSATKPSEYPEGILPEIAIAGKSNVGKSSLINTLV
                     NRKNLAKISSQPGRTQTLNFFRVNERISLVDLPGYGFAKAPLEVRKAWKPMVETYLQT
                     REAIRLVILILDSRRGLTSEDSDLLDWLDYHNIPTLIVLTKADKLSQLERARQKRNLS
                     ANPLLSRKPILFFSALTGEGKSELGGQIAKVLA"
     gene            complement(7442..8605)
                     /locus_tag="VEL68_00250"
     CDS             complement(7442..8605)
                     /locus_tag="VEL68_00250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXZ34426.1"
                     /translation="MVTKKSVEIPPFIVMEVMEKAASLERRGENIIHLEVGEPDFDTP
                     ECIKEAAIRAIRDGKTHYTHSLGVVELREAIAEDYHKRYGVEVSPDQILVTSGTSPAM
                     LLLFMSLLEPEEQVILPNPYYACYPNFVRMVEGEPVFINVYEEEGFQYRPEEIKPLIG
                     QRTKAIFVNSPANPTGNLTPMERMKAIADFGRTVISDEIYHGLVYEGKEHSILEFTRN
                     ALVINGFSKQYAMTGWRLGYLIVPPEFIRPLQKMHQNFFISANAFVQWAGIAALKEAD
                     ADVIRMKSLYNDRRKFIIKRLRELGFGITVEPTGAFYVLANAKRYTRKSFDFAFEVLQ
                     KAKVGCTPGIDFGSNAEGYLRFSYANSIEKIHEAMDRLEAYLQENFWAREKKP"
BASE COUNT         1941 a         2599 c         2369 g         2226 t
ORIGIN      
        1 tgatagaaag gaggaataaa aaaaatatga gtttacggat tctgcccatc gcctaaaact
       61 ccagcgattc tcccacggag ccgaaaaaga atcgttctcc gaattccgcc agcagccggg
      121 acgccgcctt cggccctgta cagtgcgaca ctccgatgcg ttccacgccg tattttttaa
      181 gggtcccgac cgtctcttcc atctgcgcag gcgaagcaaa atccaggtga gtccccccaa
      241 gaatggcgta aatcctctcc cggccagtct tttcgatcac atagtccaat atgttgatca
      301 tcccggaatg cgcacacccc agaaggatca ccagtccctg gcgcgtgtca atcaacaggg
      361 attggtcatc catgaaggga tcgggaatca atttcccgtc tatttcggca aaaagccttg
      421 ggtccccctt ttcgaaaggg gtccggcgcg gaatttcgcc ggtcaggaac atctcccggg
      481 ttacctcctt gaagcctttt tccagaatga accttcctcc caaggtctcc aggtagggtc
      541 ggacgaactt taatccggca taacgccgga attcttttcc gtctttccgg gagatgttgt
      601 agcggacgga gaagatatcg ggatgggcca ggatgtccac ccctcccagt acccctagga
      661 tggaggccaa tccccccgtg tggtcctggt gtccgtggct aaggaagatt ttttgtatgg
      721 ccagggggtc cttgtcaaag gtcagaagat tagggatgag cgcatctccc cgacccgtat
      781 caaagaggaa attgcccctt tcggtttcca cataggcgga gaacccgtgt tccccgattc
      841 cccccttcat ggtgaccgtg ttttcacaga taatggtgat tcgacctttc atagtccctt
      901 aaaagtttcg agtctcgcgt tttgggcttc gtgttttaac ccgaaattcg aaacccggaa
      961 ccggaaacag gttgttggtt aaaagcaacc cagctggcag ctggttatct tgatcttcag
     1021 ctggtttagc aggttgccga tttccttggt ggacacgctg cattccttgg caattttaaa
     1081 ggccatggcg caggggattt ttccctctct ggctgcttcc ttcagcttgg ccataatttc
     1141 ttcttgtttc acccttaccc ccccccattt gactcgttcg gacgaatgcc ttgtccctgt
     1201 atttttttta aagactataa catatcggaa aaggcgagga aaagacaaaa aagggagagc
     1261 ctttccggct ctcccttgct ggaaggaggt tgaatgaagt ttttaactac caacgggggc
     1321 agttgccggg acccattccc cgaccgtggc caaagccgcc tttcattccg ccgccgggac
     1381 cgaagccgag tcccatagcc gcgacctgag cttgctgctc gggggtgagg atctttcgca
     1441 tttccagccg atgtttggtg cctttttcct gcatctgcgc tctcagggcg ttaatctctt
     1501 tctgtttggc caggattttc ccctcatcgg gagtggtctg ggcccacaga gtcctgagtt
     1561 ccagctgttt gatctgcatt tcattcctca ggggaaggtt ttccttgaag aagctttccc
     1621 gcatggcctg cagcttttgg gtctgttcag cggtcaaatt cagattcgct gcccaagacc
     1681 ccctggggcc gtatccggga ccatattcgg caccaggacc aggcccgtat cccatgcccc
     1741 ttccccatcc ttgggccatg gccattccag cggtaaccag cagggcgact accatcaatc
     1801 caacgatcat ctttttcatt ttctttctcc tcttcttttt ttttgctcgc tgttctcgct
     1861 cgcttttgat tttcaatttt agcaaccccc gtgccaaggc cccggtctgg ccggtttttt
     1921 gttttaaggc acggaaagca tgaaacaatt tttcttgaga attccacttg ggcaaaaaac
     1981 ctcgcgaacc ggaggtgtaa aaattgtcga gcccaatgcg gcctattcat ataaagctaa
     2041 atgatttcgg ttctgtggaa cttctttggg ctttcgacga aatctacggg atcgacaaaa
     2101 tcgtcgaaag agaaaaaacg cttgccttat tcttgttttt aaaatataaa atgttcaggg
     2161 caaaattcaa cagggaggta tggatcatga aaatggcgaa ggcttggatg gtttttgtgc
     2221 tcctggcagg agctctcgct ttgcctttaa caagctgggc caaaacggag atcgtctggt
     2281 ggcatgccat gggcggtttc ctgggggaga gagtgaatga ccttaccaac aaattcaacg
     2341 cgagccagag tgaatatgaa atcaaggcga tcaacaaggg gagttacgcc gaagcgctga
     2401 ccgccgggat tgccgcttac cgggccaaga cccaccccca aatcatccag gtctttgaag
     2461 tcggaaccca gaccatgctc tcttcgggag ccatctaccc ggtctatcaa ctcatgaaag
     2521 accaggggat caaggtcgac tgggaggatt tcctcagtgt ggtgaagact tattactcca
     2581 agggcgggaa cctttattcc ctgcctttca attcctccac tccaattctc tactataaca
     2641 agaccgcctt cgagaaggcc ggcctcaatc cccaggttgc ccccaagacc ttcgaggaca
     2701 tcgaaaaggc cgcgaaggcg gtggtatcca agggcgccgc caagatcggg tttaccgtgt
     2761 cctggccctc ctggaccctc atggagaaca tgcatacctg gaacgaccag cccttcgcga
     2821 ataataataa cgggattgcg gccatggcca cggaactgaa gttcaacggg aaactcgggg
     2881 tcgaggttat ggaaatcctc agccggtggc agaaggaagg tatcttcacc tactccggcc
     2941 gggcggccaa cggggatcag cccaccatca acggggatgc cgctatcagc ctggcctcga
     3001 cggctctcgt cggcaccatg acccggacct cgaagttcgc ttggggcacg ggaaacctgc
     3061 cgaagctgag cgggtacccc cagggcaatt cgatcatcgg cggcgccagc ctctgggtca
     3121 tgaaggggca caacaaggag cagtacagag gagtggcaaa attctttgaa ttcctggggc
     3181 agccggagca gcaggcctgg tggcatgcca acacgggcta tttcccgttg acccgcgcgg
     3241 cgattaaggc tctcccggaa gaccatttcc gcaagaaccc caacctctgg accgcctttg
     3301 cccagatcac cggcggcaag acgaccccca acagccaggg gatccgcctc ggaaacttcg
     3361 tggccatccg ggacacgatt gaaggggaga tggaaaacat cttctccggg aagaaaaccc
     3421 ctaagcaggg cctggatgac gcggtcaaga agggcaatca gaccctgaag gaatttgcct
     3481 cgatgtataa ataatccgtt ccgtaattga ccttccgtag gggcggttcg caaaccgccc
     3541 ctgcgtccca gtatgcttct ccctctttga accggagggc tgtcgcggaa tgaaaaaaag
     3601 catcttcaat aatccgatcc tcccctacct gctggtcgct ccccagcttc tcgtcgtcct
     3661 ggtctttttc ttctggccgg cctttgaaac gcttcatctg tcggtattca cgatctctcc
     3721 cttcggggac cggcagattt ttgtggggct ggacaatttc atcgctcttc ttacctcttc
     3781 cgaatactac cggagcgtgt accactcctt cctcttctcc ctgggagtca cgttctcctg
     3841 tcttaccctg gcccttttcc tggccgtcct ggtcaaccag aagatccggg ggttggcctt
     3901 ttaccgttcc gccatcctct ggaccttcgg catggccccg cccatttcgg gggtcatttg
     3961 gctcttcatc ttccacccca cttacgggat cctgaactac cttctgtccc aggtcacctc
     4021 ctacgagttc aactggctcc tcaaagactg gctagccttg gtcctggtga tctttgccgc
     4081 cagctatgcg cacctggggt acaacgtggc cttcttcctg gccgccctgc agggcatccc
     4141 caacgcggtg gtggaagcgg cacgggtgga cggggccggc ggcctgcggc tcttccgttc
     4201 gatcaccttc cccctgctct cccccgtgac cttcttcctg ttcatcatga acatggtctt
     4261 ctccttcttc gagaccttcg gaatcatcca cgcggtcacc cagggaggcc cgggggacgc
     4321 tacgagcatc atggtctaca agacctacgt cgacggattc gtccatatgc gcatgggcct
     4381 ctccgcggct caatcattca tcctcatggc cctggttatc tttttgaccg tcctgcagtt
     4441 tcggtacacg gagaagaagg tttcctattc atgaacccct cattcccggc gtcgaaaaaa
     4501 gcaaggcttg acgggtcgaa ggtggtcgtt caccttctcc tcctcgtggc cgtgctgctc
     4561 atctgctttc ccctatacta tgccttcgtc ctcagcaccc agagcatgga agaggtcatg
     4621 gtgacccccc agaagctctg gccctcgtcc tatctctggg agaattacgt caagatctgg
     4681 gaccgagtcc acatgggcca gcttctcttc aacagcgccg tggtggctct gggcgtgagc
     4741 ctggggaaga tcgccatatc catcctctcc gcctttgcca tcgtgtactt caattttcgg
     4801 gggaagcaga tcttcttctg gatgatcttc gtgaccctca tgctcccggt ccccgtgcgc
     4861 attatctcca cctaccaggt ggtcagtgaa ctgggatggg tggacactta catgggcctg
     4921 gtcatccccc tcatcgcctc cgccacggcc acctttctct tccgccagtt ttacctgacc
     4981 attccggacg agctggtgga cgcggccaag atcgacggtt cgggccccct gcggttcctc
     5041 tggtcggtcc tcctgcccaa ctccggcccc agcatggccg cccttttcgt ggtcatgttc
     5101 atctacgggt ggaaccagta cttgtggccc ctcatggcct ctagttcgga caagatgaag
     5161 gtcgtggtcg tggggatcgc cgcgaccatc cccagcggca cccagccccc ggagtggaac
     5221 ctggtcatgg ccgtggccat gatggccctc atccccccgg ttttcatcgt cctgctcatg
     5281 cagcggtatt tcatgcacgg actgctggag accgaaaaat gaatttctct cccaggaaat
     5341 gaaaattccc cccgcattcg tcgtttttcc cattctcctg gcctttggag gaccgatgag
     5401 ttgcgcgctg aagcataccg aagaaaagag ggtgtcttac tggcgaggcc gcttcccggt
     5461 cctggtcgtc gctcaccgcg ggttttctgg cgcggccccg gagaacaccc tggcggcctt
     5521 tcgccaagcc atcgagatcg gcagcgacat gatcgagctg gatatccagc tgtccaaaga
     5581 cggcaggatt gtcgtcatgc acgatgaaac cctggagaga acgaccaacg gccggggtaa
     5641 ggtagtggat cacaacctga aggagctgaa aaagctcgac gccggctcct gcttcggccc
     5701 cgaattttcc ggggagagga ttcccaccct gcaggaggtt ctggacctgg ccaaggggcg
     5761 ggtcctggtc aacatcgaaa tcaagaatcc cacccatggg cagtatccca tcaccgacct
     5821 ggcggacaaa tcgttccagg cagtgaaaaa ggcgggaatg ctggaccggg tgatcttttc
     5881 ttctttcaac cccgtctctt tggagcatat ccagaagata gaatcccgcg cgtgggtggc
     5941 cctactcttc caccgtccct ggaactcctt cctggaaatg acccgcggaa aggaatacga
     6001 agtgctgaac ctgcgcaata tccatttaac caaagaaaaa atctcccagg tccataaaga
     6061 agggatgaag gtaaacgtct acacagtgaa tacggaggag gaactggagc agttcgtaag
     6121 gtggggggcg gacggaatta tcaccaacta cccggaccgg ctcatcagaa tcctgaaagc
     6181 aacatcgccg taggggcaat tcatgaattg cccctacttt cattcgccca tatcgcttac
     6241 agattcatcg ggtgactagg catatttccc gcccatgatg taactgctga ggttctcgat
     6301 taaaatcttc tgttcggaaa tgatcgcctt caacacgtct ccgatgctga tgactcccaa
     6361 tacccgattg gtcttctttt cgaccaccgg gatgtgccgg atccgctttt cggtcatcag
     6421 ggccatgcac tcttccatgg tagtctccgg attgacgacg tatagcttcc cagtcatgat
     6481 ttccttggta tgagtttctt tggaggacct tccctccagg atgaccttgc gggcgtagtc
     6541 cctctccgac acaattccca ctaatttttc tttttccatg accagtagag cgccgatctc
     6601 cttttcgctc aaaagttgca aggcgcggta gagaatggtg tcaggagtga ccgaccaaat
     6661 ttcttgagat ttcttcgcct ttagcaggtc ctggactgtt ttcatggaat ttcctcctct
     6721 gatgttttcg gtacgggggt gacgaaaaaa aggggatgca atttgaatcc cctactataa
     6781 agataaagaa ttttctcttt tttcgcaagg ccttttctcg gatcgaaagg tcccagaccc
     6841 ggaataggaa gccgaaggga tccttaggct aaaaccttcg cgatctgtcc cccgagttcg
     6901 cttttccctt ctccggtcaa cgcggaaaaa aaaaggatgg gctttctgga gagaagagga
     6961 ttggcggaca ggttcctctt ttgtcttgcc cgttccaact gggagagttt gtctgctttg
     7021 gtcagaacaa tcagggtggg gatgttgtgg taatccagcc agtccagcag gtccgaatcc
     7081 tcggaagtga gccccctccg ggagtccagg atcaggatga ccaagcgaat cgcttcccgg
     7141 gtctggaggt aggtctccac catgggtttc catgccttac ggacttccag gggcgctttg
     7201 gcaaatccgt atccggggag gtccaccagg gagattcttt cattgacccg gaaaaaattg
     7261 agggtctgcg ttcttcccgg ctgggagctg atcttggcca ggttcttgcg gttcaccagg
     7321 gtgttgatca gggaagactt gcccacgttg gatttcccgg cgatggcgat ctccggcagg
     7381 attccctcag ggtactcgga aggtttggtg gcgctcttga tgaattcggc ggaaagaacc
     7441 ttcatggctt tttttctctg gcccaaaaat tttcctgcaa ataggcctcc agccggtcca
     7501 tggcctcgtg gatcttttcg atggaattgg cataggagaa gcgcaaatag ccttccgcgt
     7561 tacttccgaa atcgatcccc ggcgtacacc cgactttggc cttctgcagg acctcgaagg
     7621 cgaaatcgaa ggatttccga gtatatcgtt tggcgttggc caggacatag aaggctcccg
     7681 tgggctccac cgtgattccg aatccgagtt ctcttagcct tttgatgata aatttccgcc
     7741 ggtcgttgta caggctcttc atccgaatca cgtcggcgtc cgcctccttc agcgcagcaa
     7801 tgcccgccca ctggacgaag gcgttggcgg agatgaagaa attctgatgc attttttgca
     7861 aggggcggat gaattcgggg ggaacgatga gatatcccag ccgccagccg gtcatggcgt
     7921 attgcttgga gaagccattg atgaccaggg cgttgcgggt gaattccagg atgctgtgtt
     7981 ctttcccttc atagactagc ccgtggtaaa tctcgtcgga aatcaccgtc ctgccgaaat
     8041 cagcgatggc cttcatcctc tccatggggg tcaggttccc tgtgggattg gccggagagt
     8101 tcacgaagat ggctttcgtc ctttgaccga tgaggggctt gatttcctcc ggccggtact
     8161 ggaagccctc ctcctcgtac acatttataa aaactggctc cccctccacc atacggacga
     8221 agttcgggta gcaggcgtaa tagggattgg gaagaattac ttgttcctcc ggctccagca
     8281 ggctcatgaa gagcaggagc atggccggag aagtcccgga ggtcaccaga atctgatccg
     8341 gactcacctc caccccgtat ctcttgtggt aatcttcggc aatggcctcc cgaagctcca
     8401 ccacccccag gctgtgggtg taatgggtct tgccatcccg gatggcccgg atggccgctt
     8461 ctttgataca ctccggggtg tcgaagtccg gctctcccac ctccaggtga atgatgttct
     8521 ctccccgccg ctcgagggag gcagcttttt ccatcacttc catcacgatg aagggcggga
     8581 tttccactga tttcttggta accatttcgt cctctcagga tatttcattc tatttatcca
     8641 atcgaccgga gaaagtcaag tctgtcccaa gaacctttcc aacgggtcca gagttttcgg
     8701 aagcaccccg ccctacgaag tgcaggatac ccgtctgcgc gggccctgta gcattctgat
     8761 ccggggccgg caccctacaa ggggaaattc cagttgagcc ccaaaatgct cagaaaatcc
     8821 cctttaaagc attcccgcaa aagggtctcc caagatggct agcggtcttt tgggagctat
     8881 ggaggaaaga aaagcctttc gtatgagcct cctgaaagac tcttgaagca agcagaagtc
     8941 gtcattccgg gcaagcgaag cgcgacccgg aatccaggat tttaaaacga ttctggattc
     9001 cggcttacgc cggaatgacg gaaaaggaga catttgcagg aagctcgtct tccttgattc
     9061 gaatttcata ttggaaaggt ccttgccccg ggattggttt catggtaaag aataaaatct
     9121 ggattaaatc cagtt
//
LOCUS       DASPRR010000006        16415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2259045, whole genome shotgun sequence.
ACCESSION   DASPRR010000006 DASPRR010000000
VERSION     DASPRR010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16415)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16415
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2259045"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VEL68_00255"
     CDS             complement(<1..421)
                     /locus_tag="VEL68_00255"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ34427.1"
                     /translation="MRRDTAIGIGLLVFCAVLYWQAGLVPVPPFIPIGPSFYPRVMLI
                     FLAGLAVWLIGEDLFKKRSSAGEAKKAKGPAPNYRRVLWGFSIFLGYVACLDLIGFVT
                     STFLFILGLSWAVGPRNAREVPKLVAVAAGTALACYLL"
     gene            complement(430..1392)
                     /locus_tag="VEL68_00260"
     CDS             complement(430..1392)
                     /locus_tag="VEL68_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34428.1"
                     /translation="MLRKYSFALFFLVTITLLIMGLTSAGAAYPDRPIKMIVPWAAGG
                     DTDAICRVIAASMEKNLGKPVVIVNITGASGTVGAREAKKAAPDGYTILSVHDFIHTT
                     LHTGVADLSYKDFEPVCLLTSTPSVFAAYGKTPWKSMKELIEDAKKRPEQITVGATLG
                     STSHFFPAMIAQAAGIKWKYVSYEGTAPRMTALLGGHVLVGETNLTQLDKVKAGQLKM
                     FAIATAKRLPEIPDVPTLKELGIDIVYAVNRGIVAPKGTPEAALAKLEEACAKASKDP
                     AVMDSMKKQGTFVEFLDRKAYADFLQKNDKINADLAQALGYKKK"
     gene            complement(1948..2202)
                     /locus_tag="VEL68_00265"
     CDS             complement(1948..2202)
                     /locus_tag="VEL68_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34429.1"
                     /translation="MGIGGDINSIQPSFSVQGRHRAMEKILVGTGLREGPRIEKEGLI
                     QLARRILKSDADLNFLLRLETEELETLVACIRVRLELENK"
     gene            complement(2895..3179)
                     /locus_tag="VEL68_00270"
     CDS             complement(2895..3179)
                     /locus_tag="VEL68_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34430.1"
                     /translation="MLMRARQIGIKNAGREKECKSPKYSEEQNIVMIAYEFYRRVPLG
                     EDRLIGVLPERRQKPERITRDSIMNWAKMLVITMPEDEFHRNVYFLKIEI"
     gene            3573..5285
                     /locus_tag="VEL68_00275"
     CDS             3573..5285
                     /locus_tag="VEL68_00275"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ34431.1"
                     /translation="MPLDQAVRKFVKPGMALHLGLACTPPLAVIYELVRQFRGKDPQF
                     TLFSLGLTTAYSLLVHAGLLQKITTTFLGDSYPSPGPNPVFQKALQKGTVDIRHWSLL
                     AMVQRLKAGAQGLSFLPTHSILGSSMEKDNRDDFLVMDDPFRKGERLGLVRAINADLS
                     FYHALAADPQGNSLFVPPYAENLYGALGSRQGIIVTAEKIVSTDFLRAHASYVRLPGY
                     RVLAVCEVPFGGHPSGMTSHGVNEIEGYAIDNPFVFTLRQACKKRETLDAWMKEWVLS
                     CPDQSAYLKKLGPDRLKNLKDKARPDAWQDDYESIRQKLKDDEPANAMERMVGAATRE
                     IISRVKINHYRTLLAGIGASNLAAWMATYLLRQEGYEVDVMAEIGFFGYLPRPADPFI
                     FNLSNVPTCKMLTDISEVLGILMGGENQRCLGALSAAQVDRWGNLNSTIIPPNLLITG
                     SGGANDVASGASEVLVVVPQSASRFVPQVAYITAPGQKVRILVTTIGIFEKVDGEKEF
                     TLTGTLEDGKSSEQEKVRVIKEQCKWNLKIAPRLKTLGAPTQVELALLRLFDPHKFFL
                     MENS"
     gene            complement(5289..6971)
                     /locus_tag="VEL68_00280"
     CDS             complement(5289..6971)
                     /locus_tag="VEL68_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ34432.1"
                     /translation="MAQEKIWLKSYAPGVPHEIDFEKMTLPEALNRAARSYPESTALL
                     FMGKRLSYFELDRLVNRFARVLLDLGVKRGEKVALLLPNLPQTVIAIYAAFRIGAVAV
                     MNNPLYTERELEYQFNDSDSKVLVSLDLLHPRILKIKDKTNIEKVIYCHINDYLPFPK
                     KQLFPLVKKQMYRKFEPQEGFHEFFPIIQKYPDAPMETASNWDEIGAFIYTGGTTGVS
                     KGVMLTHANLSCNVQQFSAWFPDLKKGEESVLAVFPFFHSAGFTAIMNLCIWNGYADI
                     LVPRPDPSTVIEMLKKFKPNYLPAVPTIFVGLLNTPEFRQMDLSFIKGFFSGAAPLAA
                     DTLQQLKDLTGATMLEVYGLTETTPIATVTPWGGKIKPGTVGTPVPSTDVRIMDVDTG
                     RDEQKIGTPGEIVIKGPQVMKGYYKKSEETAAVLKEGWLYTGDIGMFDADGYLTIVDR
                     KKDMIIASGYNVYPREIDEILFQHPKILEACSIGVPDPYRGETVKAYVVVKPGETLTA
                     EEVIQYSREKLAAYKAPKMVEFIDALPKSAVGKILRKEVKEMDRKKSQQRKA"
     gene            7346..8287
                     /locus_tag="VEL68_00285"
     CDS             7346..8287
                     /locus_tag="VEL68_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ34433.1"
                     /translation="MDAEKVKRVAVIGAGTMGHSIAQVFAACGIEVNLADVKKEILDH
                     ALRLVKGNLKTLVEYGKVKEREVPSILGRIHTTTDIASAAKGTDFVLEAVVEIPEIKK
                     KVFKLLDEACGEETILASNTSGLEVFKIIEVRNPSRTVVTHWFAPPHIIPLVEVVPGP
                     ETSPAVLQFAAKLMKRIGKKPVVMRQFVQRFIVNRIQNAIILTALEMLENNWATPEEI
                     DLAVKTSLGVRLPIVGVLQSLDFNGLNLVNDVLKSIGKKIPLIEEKVKQGHLGPSASK
                     GLYDYGGRSEEEILRRRDTLYLKLLDFLESQGAFNPI"
     gene            8302..9501
                     /locus_tag="VEL68_00290"
     CDS             8302..9501
                     /locus_tag="VEL68_00290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXZ34434.1"
                     /translation="MGKLNRRDFLKSSATSVGSFIFLSSNYPRQAEQKKETRKDQRKF
                     TYRTLGKTGVKLPVITMGVMNSDNPNLIRAALDSEMVHLDTAHGYMRGKNEEVIGGVI
                     KGRPRDSFVIGTKIYLPRDESTGLPKETATEQFFLSRLDTSLKRLGLDYVDILYHHNV
                     WKREFALYEPALKAMEKAKKDGKVRFTGITTHRNEPEVILAAVDSKVYEVVEIAYNHR
                     QKHGAKIREALAKAGSVGLGVVAMKTLGGGEKNLGKTEARAALKWVLQDPNVHTIIAG
                     FTTFDQLELDLSIMEDLTLKPAEKALLERMRMTAGLYCQGCEQCLKSCLQRLPIPDLI
                     RSYMYVYGYRNLVEAQDLLFSLTLPPQLCQDCGSCPVKCASGFDVSARIRDVVRLREV
                     PPDFLTA"
     gene            9549..10319
                     /locus_tag="VEL68_00295"
     CDS             9549..10319
                     /locus_tag="VEL68_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXZ34435.1"
                     /translation="MKANPLKAALAAGRIQVGTWINLVRTPSILTLLKSAGLDYARID
                     MEHSSLSMETVAGMAALGRALDFPILVRPPVGSREWITRLLDAGVWGLHIPQVDNPDI
                     AREVVKAARYAPLGLRGMAGLGPHTDFETSSDLSSQLRSLNEQVHITVMLESAEAFRH
                     LEGIVGMPGIDAVTLGPSDLAQELGVLGTPDQRRIIDEYRGRMIEAARKNGKDVAMLV
                     QSVDEAKRWIQAGVKIIVYASEVDILKNGFAAAVKQLR"
     gene            10424..10999
                     /locus_tag="VEL68_00300"
     CDS             10424..10999
                     /locus_tag="VEL68_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34436.1"
                     /translation="MKIISDHLRRGNHSEKKILTLMLVILFFLLHDKVVAETYKYVDE
                     NGSVYFVDSIDKIPAQYRRNTQILREINREEKVPKKLIAPVPEKSLLGRWEYQGSENG
                     NSYTVIMSLRPQGTFDGNGYKKMDGKTLVWMFKGNWWVMEGDLVLTYTWSSAPEPKRG
                     RTQYDAILEVNDQFLEVKSGRTGEIVRYTRK"
     gene            complement(11078..12055)
                     /locus_tag="VEL68_00305"
     CDS             complement(11078..12055)
                     /locus_tag="VEL68_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34437.1"
                     /translation="MNIRKNSRLCIMACLALVFLFSAALASGQTYPDKPITIYCAYAA
                     DTTDLTTRSLAEGVEKVFGVPVVVENKPGGGSTVCAGLLATKKPDGYTLGVISTGALR
                     LLPHLMKISYDPLKDFTLIMQYSRFLGGLCVLSESPIKTVDDFIAYAKAHPNLSYGSP
                     GMYSQQNLAVELFAQCKGLTFKHVPYKSGAETSTALLGKHIDFAAGSGTHLRYVKQGV
                     FRELLTYNSNQRSHYNPKLPTLEEIGCQDFPANAMILVGPKGLPEPIAKKLGETFKKV
                     SESAAFQSKLTSLDLPYDYKGQAQLEREIPELYAAGKVLLTKMGAKKAE"
     gene            12468..12938
                     /locus_tag="VEL68_00310"
     CDS             12468..12938
                     /locus_tag="VEL68_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34438.1"
                     /translation="MKCLSAVLLGAFFAAWIPILQSCSLITLPLTSTVFGGAQLALKG
                     AELQKEISKADVRKSFESPFEKTWNMAAITLVNLHIEIAQIGKTPDGNGGLIEGRARK
                     IKVKVIAIEITENITEVGIWTDYDKALAELIAEKIKEEVQKQDHPIQGIGSEVE"
     gene            complement(13082..14536)
                     /locus_tag="VEL68_00315"
     CDS             complement(13082..14536)
                     /locus_tag="VEL68_00315"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXZ34439.1"
                     /translation="MLSLPFRSAKQLASLIRQKKIGCLELLDLYLKRVEQYNPKLNAI
                     IFKDVEGARKRAKMADRALARKEVWGPLHGVPMTIKESFDVAGMPTTWGVPLYRDNYA
                     QKNSLAVDRLLKAGVILFGKTNVPLYLGDWQSFNEIYGTTNNPWNVTRVPGGSSGGSA
                     AALAAGLTGIDAGSDIGASIRNPAHYCGVYGHKPTYGVCSPRGHALPGVVAVGDISAI
                     GPMARSAGDLAIGLAAMAGPDLIDGIGWQLHLPGPAKKQLREYKVSVVYTDPEAEVDE
                     EVQKGIRAAADFLRKNKAKVVEDARPAFGSGEAFRNYVQLLRSATSGRLTLEQFQKNL
                     AAFNTLESDDNSYQAQAIRAQAMYHKDWLAYNEGRHKMRLAWAEFFKEYDLLLCPTAT
                     TAAFPHNQKGERWERMIMVNGNPQPSTTQMFWAGYSCNFYLPSTIAPAGFTTEGLPVG
                     VQIVGPQYGDYTCIYFAQLLEKDFQPFVSPLGFE"
     gene            14667..16238
                     /locus_tag="VEL68_00320"
     CDS             14667..16238
                     /locus_tag="VEL68_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ34440.1"
                     /translation="MRVLLINPPYPIVESLTMPLGLLYLAARLEQTGCQVHLEDLQLC
                     RSPLTRLEETLHHQKPALAGITSFSLNLTMASKILQKVKRFSPDTVTVWGGPHVSFDD
                     REVLHQHPWVDVVVRGEGEETLAEVVDRVRLGKDFEGVLGTTCRGKDGDFSINPSRPF
                     REDLDQLPRPAWHLLKLSQYRAFGDGASLMTSRGCPHRCVFCVGRKMIGAKGRFRKPE
                     AVVDEMEALAQQGFRRLRIEDDLFTFRRERALAICKEMDSRGLTIRWRAYSRVDTIDA
                     ELLRWMKRTGCERLLYGAESGSPEILKKIRKGITPDQTRQAVEMTREAGIGVLASFVL
                     GLPGETPQTLQQTLEFAESLQVPYSLNLLTPYVGTEIRERAEDLGIKVLSDDWRHYGQ
                     GDPLTSTPTVTPWHLSRAVGSYRKGLRKYLEDLLEQDRLGTLTKQNSQELERNRHWAF
                     LRRLIGGEILERYGSVLPGSGRSAAEELAKSLAFPLQMTPGEVEKHLDALVRGGNIRP
                     EHNQNGMTRWEWAES"
BASE COUNT         3795 a         4278 c         4543 g         3799 t
ORIGIN      
        1 agagcaggta gcacgccagc gccgttccgg cggccaccgc gacgagtttc ggaacctcgc
       61 gggcatttcg cggcccgacc gcccagctta atcccagaat gaacagaaag gtggaggtta
      121 cgaaaccgat gaggtcgagg caggccacat agcccaggaa gatcgagaac ccccaaagga
      181 cccgccggta attcggcgcg gggccctttg cctttttcgc ttcccccgcg gacgaccgtt
      241 ttttgaaaag gtcctcgcct atcagccaga cagccaggcc cgccagaaag atcagcatga
      301 cccgtggata aaaagagggc ccgatgggta tgaaaggagg aacggggacg agaccggcct
      361 gccaataaag aacggcgcag aaaaccagca ggccgatccc gatagcggta tcacgtcgca
      421 tgagaccccc tacttcttct tatagcctag agcctgggcc aggtcggcgt tgatcttgtc
      481 gttcttctgg aggaagtcgg cataggcctt gcggtccaaa aactcgacga aggttccttg
      541 tttcttcatc gagtccatga ctgctgggtc tttggaggcc ttggcgcagg cctcttccag
      601 cttcgccagt gcggcctccg gggttccttt gggagccacg attccacggt taacagcgta
      661 cacgatgtcg attcccagtt ccttcaaggt gggtacatct ggaatctccg gcagccgctt
      721 ggccgtggca atggcgaaca ttttcaactg gcccgctttg actttgtcga gctgggtcag
      781 gttcgtttct ccgacgagga cgtgtccccc cagcagggcg gtcatccggg gagccgttcc
      841 ctcgtagctc acgtacttcc atttgatccc agcggcctgg gcgatcatgg ccgggaagaa
      901 gtggcttgtg gaccccaggg ttgcacccac ggtgatctgc tcgggccgct tcttggcgtc
      961 ctcgatcagc tccttcatgc ttttccaggg ggtcttgccg taggctgcga aaaccgaggg
     1021 ggttgaagtg aggaggcata cgggttcgaa gtccttgtag ctcagatcgg caactccggt
     1081 gtgcaaggtc gtgtggatga agtcgtggac cgagaggatc gtgtacccgt ctggagcagc
     1141 cttcttggct tcccgagccc cgaccgttcc cgaggccccg gtgatgttga cgatgaccac
     1201 gggtttgccc aggttctttt ccatgctggc ggcgatgacc cggcagattg catccgtatc
     1261 gccccctgcg gcccagggca cgatcatctt gatcggccgg tccggataag cggctccggc
     1321 ggacgtcagg cccatgatta gaagcgttat ggttaccagg aagaaaagtg caaaagaata
     1381 cttgcgaagc atgtattttc ctcctttgga ttgaaagtgg atttaagcct ctttgggaat
     1441 gaaattagaa tacgggggga agaacagtgt gtcaagggga aagaacccac ttgcccaccc
     1501 ccagtttttc gtgcttgttg gggttcaggg tggaggaatt tggatcatca ggctcggcgg
     1561 gtgcttatcc ttccatgaaa ctcttcccca tgttccgcag gcagaggcct gagaggagat
     1621 tcctccaaag aaaagagaac caaagcattt ttccgtttca atccccccag gacgggcccg
     1681 atccaatgga aggaccaccg cctcaggatg atgatccaaa tttctccgcc ctgaacccca
     1741 tgagatcccc cctcctatca gagaccttat tttcaaagga ctctttttta agaactgggg
     1801 gtgggcaagg gttaccgagt ggtcgagagt cgataaaaaa gaggcaaggc caatatcgac
     1861 cttgcctctg tccgaagctg gaatgaatct ctctttttcg gaagcaccca ccgaaagatg
     1921 ctcccttata tctttgaatt tgccttatta tttgttctcg agttctaacc taacccgaat
     1981 acaagcgacc aaggtttcaa gttcttcggt ttcgagcctc agaaggaaat ttaaatccgc
     2041 atccgacttc agaattctcc tggccagctg gatcaaccct tctttttcta tcctggggcc
     2101 ttcccggagc ccagtgccca caagaatttt ctccatagcc ctatgcctac cctgtacaga
     2161 aaaacttggt tgaatggagt tgatatctcc gccgattccc atcttattgg cccccttttt
     2221 gtccggattt gtctcttgga ggaatgaaag agagtccgga aagatccccc aaataacgtt
     2281 tcagattcaa agaaattttc aggtgtgtgg gagtggggtt tgggagcgag agtgcaggag
     2341 gttttagttc acttcctctc ttcagaattt tataatcgta ataaacggcc atgtagtttt
     2401 caaattccct gcaatccctc ttcagtaggg acagctcgtg gatccccacc ctttttaatt
     2461 gattcaacac atcgcgtcga gtcatcatgt caatcaccct gcaaaaaatt cgccaaagcg
     2521 tttattccct caaattcctg gaaatgattc atgtttgaaa taggcatcaa attatatatc
     2581 cccttccatc catagagccc attccctaaa aaggtgaaaa tgtcccatag caaaagagcc
     2641 gggatttcct tctatgcctc aaccttatta aggttcatca atcgaaacct ggcggaggcc
     2701 gattcttact gacaaggata tcagcacctg ccgcagaata caggagttca ttctcttcgt
     2761 aacctgggtt tagaccctat gcaaaaggag ttgagaggaa agtagtcaaa gaggaccgat
     2821 ggccaaaaaa gacctccggt tttaaacaaa gccatgggtg cggccatcct cgaatttctg
     2881 gccccccctt cttactatat ttctattttg aggaagtaaa cattgcgatg aaattcatcc
     2941 tccggcatcg tgattaccag catttttgcc cagttcatta tcgaatctct agttattctt
     3001 tccggtttct gtcttctttc gggcaaaaca ccaatcaggc ggtcttcccc taggggtaca
     3061 cgacggtaga attcataagc aatcatgacg atattttgct cctcggaata ttttggagat
     3121 ttgcattcct tttcccttcc agcatttttt ataccaatct gacgagctcg cattaacatg
     3181 ctgtaattat tagcatttcc ctaaattatg gatttaagga aaagccgctt ttacgtaaga
     3241 ttttgggaca tgggccgtta aggatcttct aggtgcctgt aaccaataga aaaatttaaa
     3301 ttctggggtg tggctttttt acatttttac caggctggta caattaattg ggcccgagga
     3361 ttgagaaaag aagaaaaatt tgcagtgtgg atgttgattg gaaagaaaaa tgcctttggc
     3421 gatcacctgt tttcctctgc tctgggggca agtccactaa ccttcttgac ttttgaccgc
     3481 acatcctcta gtttcaggcc atgggagatg cttttgaaag gacactcagg gggattattc
     3541 ctctcaggtc gaccgaaggg cagagtaaag taatgcccct tgaccaagcg gtccggaaat
     3601 ttgtcaagcc cggtatggcc ctccatctgg gcctggcatg tacgcctccg ctcgcggtca
     3661 tctacgaact ggtccggcaa ttccggggca aggaccctca attcacgctc ttctccctgg
     3721 gtctgacgac ggcttattcg ctgctggtcc atgcgggcct gcttcaaaaa atcaccacca
     3781 ccttcctggg cgattcctat ccctcaccgg gcccgaatcc cgtttttcaa aaggccctcc
     3841 agaaaggaac ggtagacatc cggcactggt ccctgctggc gatggttcaa aggctcaagg
     3901 ccggcgccca gggattaagc ttccttccca cgcactccat cttagggagc agcatggaaa
     3961 aggataaccg cgatgatttc ttggtcatgg atgatccctt tcggaaaggg gaaaggttgg
     4021 gcctggtaag ggcgatcaac gctgatctct ccttctacca cgccctcgcg gcggaccctc
     4081 aggggaacag cctttttgtt cctccttacg ccgaaaacct gtatggagct ctgggaagca
     4141 gacagggcat tattgtcacc gcagagaaga tcgtttccac agattttctc cgggcccatg
     4201 ccagctatgt tcggcttccg ggctatcggg tcctggcggt ctgtgaagtt cctttcgggg
     4261 ggcatccgag cggaatgacc tctcacgggg tcaatgagat cgaggggtat gccatcgaca
     4321 atccctttgt cttcaccctg agacaggcct gcaagaagag ggaaaccctg gacgcctgga
     4381 tgaaagagtg ggtgttgtcc tgtcccgacc agtcagctta cttgaaaaaa ttgggcccgg
     4441 atcggttgaa aaacctgaag gataaagccc gcccggacgc ctggcaggac gattatgaat
     4501 ccatccgtca aaaactgaag gatgatgagc cggccaatgc catggagcgc atggttggtg
     4561 cggcaacccg ggagatcatc tcccgggtga agataaacca ctaccggact ctattggccg
     4621 gaattggagc ttccaacttg gccgcatgga tggctaccta cctcttgagg caggaaggct
     4681 atgaagtgga tgttatggcg gagatcggct tctttggata tttgccccgg cccgcggatc
     4741 cgttcatttt caatctttcc aacgtaccta catgcaagat gctgaccgat atctccgagg
     4801 ttttgggcat tctcatgggt ggggagaacc agcgctgcct gggggctctg agcgcggccc
     4861 aggtggaccg gtggggaaac ttgaactcca caatcattcc tcccaacctt ctgatcaccg
     4921 gctcaggggg tgccaatgac gtggcctccg gtgcctccga ggtgctggtg gtggtcccgc
     4981 agtcagcctc ccgcttcgtc ccgcaggtgg cttacatcac ggctccgggg cagaaggtga
     5041 gaatcttggt gaccaccatt ggtattttcg aaaaagtgga cggggaaaag gagttcaccc
     5101 ttacgggcac cctggaagat gggaaatctt cggagcagga aaaggttcgg gtgatcaagg
     5161 aacagtgcaa gtggaacttg aagatcgccc cccggctgaa aactctcggg gcgccgacgc
     5221 aggttgaact tgccctgctc cgcctcttcg acccgcataa atttttccta atggaaaact
     5281 cctaattttt acgcttttct ttgctggctt ttcttccgat ccatctcctt tacttccttc
     5341 cgcagaatct tccccaccgc gctcttgggg agggcgtcga tgaactccac catcttgggg
     5401 gccttgtagg cggcgagctt ctcccggctg tattggatga cctcctccgc agtgagggtt
     5461 tccccgggtt tgaccaccac gtaggccttc accgtctctc cccggtatgg gtcgggaacg
     5521 ccgatgctgc aggcctccag gattttggga tgctggaaga ggatttcgtc aatctcccgg
     5581 ggatacacat tgtacccgct ggcgatgatc atatctttct tgcggtccac aatagtcaaa
     5641 tagccgtccg cgtcaaacat gccgatgtcg ccggtataga gccagccttc tttaaggact
     5701 gcggccgttt cttcggattt cttgtaatac cccttcatga cctgggggcc ctttatgaca
     5761 atctccccgg gagtcccgat tttctgctcg tcccttccgg tgtccacatc cataattctc
     5821 acgtcggtgc tgggaactgg agtccccacc gttccaggtt tgatctttcc gccccagggg
     5881 gtgaccgtgg cgatcggcgt ggtttcggtg agcccataga cctccagcat ggtggccccg
     5941 gtcaagtctt tcagctgctg cagggtgtcg gcggccagag gggcggctcc ggaaaagaac
     6001 cccttgatga aagacaaatc catctgtcga aattcagggg tgttgaggag acccacaaaa
     6061 atggtgggca ccgccggcaa atagttgggt ttgaattttt taagcatttc gatgaccgtg
     6121 gacggatcgg ggcggggcac gaggatgtcg gcgtagccgt tccatatgca gaggttcatg
     6181 atggcggtga agccggcgga atggaagaag gggaagacag ccagcacgct ctcctccccc
     6241 ttcttcaggt ccggaaacca ggcgctgaac tgttggacat tgcaggacaa atttgcgtgg
     6301 gtcagcatga cccctttgct caccccggtc gtccctccgg tatagataaa agcgccgatt
     6361 tcgtcccaat tcgaggcggt ctccatcggc gcatccgggt atttctggat gatcggaaaa
     6421 aattcatgga atccttcttg aggttcaaat ttccggtaca tctgcttctt caccagaggg
     6481 aaaagctgtt tcttggggaa ggggagataa tcattaatat gacaataaat aaccttttcg
     6541 atattggtct tatccttgat cttcaaaatc ctggggtgca gcaggtccag ggaaacgagg
     6601 accttggaat cggagtcatt gaactggtac tccagctccc gttcggtgta gagcgggttg
     6661 ttcatcacgg cgacggctcc gatgcgaaaa gcagcgtaga tggcgataac cgtctggggg
     6721 aggttgggca atagcaaggc caccttctcc ccccttttga ctccgaggtc caggagaacg
     6781 cgggcgaagc ggttcaccag ccggtccagt tcgaaatagg aaagacgttt gcccatgaac
     6841 aagagagccg tcgattcagg ataactccgg gcggctctgt tcaaagcctc gggcagggtc
     6901 atcttctcga aatcgatctc atggggtacc ccgggggcgt aggacttcaa ccagatcttc
     6961 tcttgggcca tgtcgttccc ctccttccgg gtttatagaa gctgaacccg ctttacaaat
     7021 ctttccttgg atgtaagaaa ttcgattggg taggagtccg gaatgcagat tgcgaatctg
     7081 ggagtctcct tgctattgcg gcgttttatt ccgcagtccg cattccacat tccgcattgg
     7141 gaagggtcct tctgaaaatc catttgcatg acaggctcca gaaggttcag gtcagatttt
     7201 cagcttgtga tcgtttaagg agagagatag cgagaaaatt agaggaaaaa ttttgtcttg
     7261 tcaagataaa tatgatatga gtgggagggt cggtcatatg gcggccgatg gaaggaaaaa
     7321 tagaactgcc cgaggggaga tgggaatgga tgcggaaaaa gtaaagcggg tagcagttat
     7381 cggagcgggg actatgggcc attccatcgc ccaggtcttt gccgcatgtg gaatcgaagt
     7441 aaatctggca gatgtaaaaa aggaaattct cgatcatgcc ctccggctcg taaagggaaa
     7501 cttgaagacc ctggtcgaat atggcaaggt gaaggagagg gaggtcccct ccattctggg
     7561 gcggattcat acgaccacgg atattgcgtc agcggcgaaa gggacggatt tcgtcctgga
     7621 ggcggtggtg gaaattcctg aaataaagaa gaaggttttc aagctgctgg acgaggcatg
     7681 cggagaggag acgatcctag ccagcaatac ctcgggcctg gaggttttca agatcatcga
     7741 agtccggaac ccttcccgca ctgtggtaac ccactggttt gccccacctc acatcatccc
     7801 cctggtggag gttgtcccgg gccccgagac ctcgcccgca gttctccaat tcgcagccaa
     7861 gctcatgaag aggatcggca agaagccggt ggtgatgcgc cagttcgtgc agcggttcat
     7921 cgtgaaccgg atccaaaacg ccatcatcct cacggcgctg gaaatgctgg agaacaactg
     7981 ggccactccc gaggagatcg atttggcagt gaagacgagc cttggagttc gcctccccat
     8041 cgtgggagtc ctccagagcc tggatttcaa cgggctgaat ttggtaaatg atgttctgaa
     8101 aagcataggc aaaaaaatcc ccctgatcga agaaaaagtg aaacaagggc atttagggcc
     8161 aagcgcctcc aaggggctct atgattatgg cggtcggtcg gaggaagaaa tcctccggag
     8221 gagggatacc ctttacctca aattgctgga cttcctggag agccagggag cctttaatcc
     8281 catctagata aaggagaggg catggggaag ttgaaccgtc gagatttctt aaagtcttcg
     8341 gcgacaagcg tgggaagctt catctttctt tcgtccaact acccaagaca ggccgagcaa
     8401 aaaaaggaga cgcgaaagga tcaaaggaaa tttacctacc gcaccctcgg aaaaaccggc
     8461 gtgaaactcc cggtgatcac catgggggtg atgaactcgg acaatcccaa cctcatccgg
     8521 gcggccctgg attctgaaat ggtgcacctg gacactgccc atgggtatat gaggggaaag
     8581 aacgaagagg tcatcggcgg ggtgatcaag gggaggcccc gcgattcttt tgtcatcggc
     8641 accaaaatct acctgccccg ggacgagagc accggcctgc ccaaggaaac ggcaaccgag
     8701 cagttcttcc tgagccggct ggataccagc ctcaaacgcc ttggactgga ttatgtggac
     8761 atcctctatc atcacaatgt gtggaaaagg gaatttgctc tgtatgagcc cgccctgaaa
     8821 gcaatggaaa aagcaaaaaa ggatggaaag gtccggttta cgggaattac cacccaccgc
     8881 aatgaaccgg aggtcattct tgcggctgtc gattccaagg tgtatgaggt agttgaaatc
     8941 gcttacaacc atcgccagaa gcatggtgcc aagattcgcg aagcccttgc caaagcgggg
     9001 agcgtgggct tgggagtggt ggccatgaag actctcggcg gcggggaaaa aaatctggga
     9061 aaaacggaag ctcgggcggc cctgaagtgg gttctccagg accccaacgt tcacacgatt
     9121 atcgccggct tcaccacttt tgatcagttg gagcttgatc tgtccattat ggaggacctg
     9181 accctgaaac cggccgaaaa ggctcttctg gaaaggatga ggatgactgc gggcctgtac
     9241 tgccagggct gcgagcagtg cctgaaatcc tgcctccaga ggcttcccat ccccgatctc
     9301 atccgaagct atatgtatgt ttacggctac cggaatctgg tggaggcgca ggacctcctt
     9361 ttttccctga cgctgcctcc acaattgtgc caagattgtg gctcctgtcc ggtgaaatgt
     9421 gcgagcgggt tcgacgtttc cgcccgcatc cgggatgtgg tccgcctccg ggaggttccc
     9481 ccagattttc ttaccgccta aatccatgaa gcggaggcgg atttggttca aggaggtcaa
     9541 tcttgccgat gaaggccaat cctctgaaag ctgcgttagc tgccggtaga attcaagttg
     9601 ggacctggat caacttggtc cgaaccccgt cgattctgac cttgctaaaa agtgccggtc
     9661 ttgactatgc ccgaatcgat atggaacact cgtccctctc catggaaacg gtcgcgggta
     9721 tggcagccct cggccgggcg ttggattttc ccatcctggt tcgcccgccc gtgggcagcc
     9781 gggagtggat cacccgcctt ctggatgcag gggtgtgggg tctccatatc ccgcaggtgg
     9841 ataacccgga tatcgcccgt gaggtggtaa aggcggcccg ctatgctcct ctgggcttgc
     9901 gcgggatggc agggctgggg ccccatacag atttcgagac ttcttccgat ttatcctctc
     9961 aattaaggtc tctgaacgaa caggtgcaca tcacggtgat gctggagtcc gctgaagctt
    10021 tccgccacct ggaagggatt gtgggcatgc cgggaatcga cgcggtcacc ttggggccgt
    10081 ctgacctcgc ccaggaattg ggggtgcttg gcaccccgga ccagcgccgg attatcgacg
    10141 agtatcgcgg gcggatgatc gaagcggccc gaaaaaacgg gaaggatgtg gccatgctgg
    10201 tccagtccgt tgatgaggcc aaacggtgga ttcaggcggg ggtaaaaatc atcgtctatg
    10261 catctgaggt ggatatcctg aaaaatgggt ttgccgcggc cgtcaaacag ttacggtaga
    10321 tcgggcccat cgggaaaccc gctctctatg cttcattttt ccgctcccac gccgatagaa
    10381 tattcaagga gtattcatct tcccttgcca agaataatcc ggtatgaaaa tcatttccga
    10441 tcacttaagg agagggaacc attctgaaaa gaaaattctc accctgatgc tggtgattct
    10501 tttctttctg ctccatgaca aagttgtggc agagacttat aagtacgtcg acgagaatgg
    10561 ctctgtgtat ttcgtcgatt ccatcgacaa aattcctgcc cagtacagaa gaaatactca
    10621 aatcctccgg gagatcaatc gagaagagaa ggtgccaaaa aagttaattg cgcctgtgcc
    10681 ggagaaatcc ctgcttggca ggtgggaata ccaggggagt gaaaacggga attcctacac
    10741 cgtgattatg agccttcggc cgcaagggac cttcgatggg aacggctaca agaagatgga
    10801 tggaaaaact cttgtatgga tgttcaaggg aaactggtgg gtgatggaag gggacctcgt
    10861 tctgacctat acctggagct cggccccgga gcccaagcgg gggaggaccc agtacgatgc
    10921 catcctcgaa gtgaatgatc aattcctgga ggtgaaatca gggagaacgg gggagatcgt
    10981 tcgttacaca cggaaatgaa tcccgtcccc cctgcaagtt cggttctcaa cttcaaaaaa
    11041 cgacctgttc ccagtcagaa gggaaccgga ttctcggcta ttccgccttc ttagccccca
    11101 ttttcgtcag aaggaccttc cccgccgcat agagctcggg aatttctctt tccagttgag
    11161 cctggccctt ataatcgtag gggaggtcca ggctggtcag cttgctctgg aaagcggcgc
    11221 tctctgaaac ctttttgaag gtctcgccga gttttttcgc tataggttca ggaagccctt
    11281 tgggtccgac gaggatcatg gcgttggccg gaaaatcctg gcagccgatc tcctcgaggg
    11341 tggggagttt cggattataa tggctccgtt ggttggagtt gtaagtcaga agttcccgga
    11401 ataccccctg tttcacatag cggaggtgcg tgcccgaccc agcggcaaag tcgatgtgct
    11461 tgcccagcag ggcggtgctt gtttccgccc ccgatttgta ggggacgtgc ttgaaggtaa
    11521 gccccttgca ttgggcaaag agctctaccg ctaaattttg ttggctgtac atgcccgggg
    11581 acccgtaaga taggttggga tgggccttgg cgtaagcgat gaaatcgtcc accgtcttga
    11641 tgggcgactc gctaaggacg cagagccctc cgaggaagcg gctatactgc atgatcagcg
    11701 taaaatcctt caacggatcg taggagatct tcatcagatg ggggaggagt cttaaggcgc
    11761 ctgtggagat cacgcccagg gtatacccgt cgggtttctt ggtggccagc aggccagcac
    11821 ataccgtcga gccgccgccc ggcttattct ccaccacaac agggactcca aagacctttt
    11881 caactccctc cgccagactg cgggtggtca aatccgtggt atcggcggcg taggcgcaat
    11941 agatggtaat cggcttatcc gggtatgtct ggccggaagc aagagcagca gagaatagga
    12001 aaaccagagc caggcaagcc atgatgcata aacggctatt ttttcttata ttcatattga
    12061 caacctccta tccatctttt ccatatcaac ccaattctcc ggccggccct tcaatctgct
    12121 ccatatctta gtagattgcc ttcgtttaat caaggtgatt tgcccgcagt aagcaatgca
    12181 gacactggac cgcaggccgc agcctccaga cttgtgacta aaggcttttc aacctggcca
    12241 aaggcgaaat gttagtcatg atccccgtta ataattcacc ctggcccggt cgcttacctt
    12301 ttgattcatt tccccggctt gaaacctgat gtttgtggtc tgaggtctga aatctatggt
    12361 ctgctatttg aatcggttca agattgatgg tacattaaaa agtggatgag caccggaagg
    12421 actaccatga ggagccagaa gacccactgg ctcgaggaat ggaattcatg aaatgtcttt
    12481 cggcagtttt gttgggggct ttttttgcag cttggattcc gattctgcag tcctgcagtc
    12541 tcataaccct tcccctcaca tcgaccgtgt ttggcggggc tcaactggcc cttaaagggg
    12601 cagagctgca gaaagaaatc agtaaggctg atgtcaggaa atcttttgaa agcccctttg
    12661 aaaaaacatg gaatatggcc gccatcaccc tggtgaacct ccatattgaa attgcccaaa
    12721 tcggaaagac cccagatggg aatggcggct taatcgaagg acgggccagg aagataaagg
    12781 taaaggtcat tgccatcgag atcacggaga atatcaccga ggttggtatc tggaccgact
    12841 acgacaaggc actcgccgag cttatcgccg aaaaaatcaa agaagaggtc caaaagcaag
    12901 atcatcccat tcagggaatt gggtccgaag tggaataagg atgtagcgat tcggaattgg
    12961 ggcggtacgg gaaagaatgt gcggcgatgg gtaaccccat ttcctctgcg gggtctttcc
    13021 cttctcttac ccaccacacc tcaatcctcc cccgcatgag ggaaggaaat ggaccgggtg
    13081 attattcaaa ccccagaggg gaaacgaagg gctggaaatc cttttccagc aattgggcaa
    13141 aataaatgca agtatagtcc ccgtactggg gccccacgat ttgaactccc accggtaaac
    13201 cttcagtcgt aaatcccgcc ggcgctatgg tggaggggag ataaaaattg caggaatacc
    13261 cggcccagaa catctgggtg gtggagggct gggggtttcc gttgaccatg atcattcgct
    13321 cccagcgctc gcccttttgg ttgtggggga aagcggcagt ggttgccgtg gggcacagga
    13381 gcaggtcgta ctccttgaaa aattccgccc aggccaggcg catcttgtgg cgcccttcgt
    13441 tgtaggccag ccagtccttg tggtacatgg cctgggcccg gatggcctgc gcctgatagc
    13501 tgttgtcgtc ggattctaga gtgttgaagg cagccaggtt cttctggaac tgctctaggg
    13561 tcaggcgccc ggaagtggcc gaacggagaa gctggacata gttccgaaaa gcttccccag
    13621 aaccaaaagc gggccgggca tcctcaacca ctttggcctt gttcttcctc aggaaatcag
    13681 cggccgcccg gatccccttc tgaacctcct catccacctc cgcttcggga tcggtataaa
    13741 ccacggagac cttgtattcc cgcagttgct ttttcgcagg gccgggaagg tgaagctgcc
    13801 agcctatccc atcgatcaag tcggggccgg ccatggccgc gaggcctatg gctaagtccc
    13861 cggcgcttcg ggccatgggg ccgatggcgg agatgtcccc gacagccacc accccaggca
    13921 gggcatggcc acggggagag cagacgccgt aggtcggctt atgtccatag acgccgcagt
    13981 aatgggcggg attccggatc gatgctccga tatcgcttcc cgcgtcgatt ccggtaagcc
    14041 cggcagcaag ggcggcagcg gagcccccgg atgatccgcc gggaacccgg gtcacattcc
    14101 agggattgtt ggtggtcccg tagatttcat tgaaactctg ccaatcacca aggtagaggg
    14161 gaacgttggt tttgccaaaa agaatcaccc cggctttcaa gaggcggtcc accgcaagtg
    14221 aattcttctg cgcatagtta tcccggtaca gcgggactcc ccacgtcgtc ggcattcccg
    14281 ctacatcaaa agattccttg atggtcatgg gcacgccgtg aagaggaccc cacacctctt
    14341 tcctggcaag cgcccgatcg gccatcttgg cgcgcttccg cgctccttcc acatccttga
    14401 agatgatggc attcaatttc ggattatatt gctcaacgcg cttcaggtat aaatccagaa
    14461 gctccaggca tccgatcttt ttctggcgaa tcagagaggc aagctgtttg gcggaacgga
    14521 agggcaggga aagcatgaaa tcctcctttc aataaaacga atcccccttt tcaatgatcc
    14581 attgactggt aggataattc catctaatat gtgcttgatt ctgagatatt tgtcaatatg
    14641 aaaccagatg tcaggaagaa atctccatgc gagttctgct catcaacccg ccttacccga
    14701 tcgtcgaaag ccttactatg ccgctcggcc ttctttatct ggcggccagg ctggagcaaa
    14761 caggatgcca agtgcatctt gaggacctcc agctttgccg ctcccctcta acccgcctcg
    14821 aagagaccct gcatcaccaa aaacccgcct tggcggggat tacttctttt tccctcaatc
    14881 ttaccatggc ttccaaaatt ctccagaaag tgaaacgttt ctctcccgat acggttacgg
    14941 tatggggagg accccacgtt tcctttgatg accgggaggt ccttcaccag cacccctggg
    15001 tcgatgtggt ggttcgggga gaaggagaag agacattagc ggaggttgtc gaccgggtcc
    15061 ggctggggaa ggatttcgag ggagttctgg gcaccacctg cagggggaag gacggggatt
    15121 tttcgataaa cccttccagg ccatttaggg aagacctgga ccaactgccc cggcccgcct
    15181 ggcatcttct taagctctcc caataccggg ctttcgggga tggtgccagc ctcatgactt
    15241 cccggggctg tccgcaccgg tgtgttttct gcgtaggccg gaaaatgatc ggagcgaaag
    15301 gaaggtttcg gaagccggag gccgtagtgg atgagatgga ggccctagcc cagcaggggt
    15361 tccgccggct gcggattgaa gacgatctgt tcaccttccg ccgcgaacgg gctttggcca
    15421 tctgcaaaga aatggatagc cggggtttga ccatccgctg gcgggcctat tcccgggtgg
    15481 ataccattga tgccgaattg ctgcgatgga tgaaaaggac cggttgtgaa agactcctct
    15541 acggcgccga gagcggaagt ccggagattc tgaaaaagat acgaaagggg atcacgccgg
    15601 accaaacccg ccaggccgtg gaaatgaccc gggaagcagg cataggggtt ctggcttctt
    15661 tcgtcctcgg gctacccggc gaaacccccc agactctgca acagaccctg gagtttgccg
    15721 aatccctcca agtcccttac agcctgaacc ttctcacccc ttatgtggga acggagattc
    15781 gggaaagagc agaggatttg ggaatcaagg tcctctccga cgactggcgc cattatggac
    15841 aaggcgatcc cctaacctcc accccaaccg taaccccatg gcatttgagc cgggccgtag
    15901 gtagttatag aaaaggcctg cgaaaatatc tggaagacct tttggagcaa gaccggctag
    15961 ggactttgac caaacaaaat tcgcaggagc tggaacggaa ccgccactgg gcctttttac
    16021 ggcggttgat cgggggagag attctggagc gttatgggag tgtccttccg ggatcgggca
    16081 ggagtgctgc ggaggaactg gccaaatctc tcgcctttcc tctgcaaatg accccaggag
    16141 aggttgaaaa acatttagac gccctggttc gcggaggaaa tatccgccca gagcataatc
    16201 aaaatggaat gacccgatgg gagtgggccg agagttgaac gacactcccg agatgtagta
    16261 ttttcccatt ggggatatgt aaaaattttc cagtcttcat tcgagtcata aatttatgac
    16321 gggaaaaagg gtgagccgat gcgggttatt ttttcgttaa tgttgcacag ccatatcccc
    16381 tactgtcgga aatccggggt gtggccggcg gggga
//
LOCUS       DASPRR010000007         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1936580, whole genome shotgun sequence.
ACCESSION   DASPRR010000007 DASPRR010000000
VERSION     DASPRR010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936580"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1741
                     /locus_tag="VEL68_00325"
     CDS             305..1741
                     /locus_tag="VEL68_00325"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ34441.1"
                     /translation="MALLTGKEYVESLKELSPEVYVRGEKVQEIWSHPLLRQTVNHIA
                     AGYDFALDPRYRASAAVQSPLTGEEVPRIQLHIQESLEDVVAKAKLTREITGRRVCAG
                     CMSNMLSVTWALIYDIDQAHSTKYFPRFREFVKSLQKKDQIIAWGMMDPKGDRSLPPS
                     QQKHSPDLRIVKQRSDGILVRGTKVHTTFGPAAHHIVVVPCRALGPEDKEYAVSFALP
                     IDAKGLKMIARPSPGPAAETKMESPLSSRYLGVEAMTLFDDVFVPEDKIFMCREWDQA
                     FKLPLYFASLHRQSKCACSAGHTDLFIGTSALAAEVNGLGMKVSHIRDKITEMMMAAE
                     TAFGCSLGAALEGKKHPSGVFLPDPVIANSGLNFIRSRMGTHLSYLHDIAGGLLSTMP
                     TEADWNHPDLRKYIEEGLRGSPKYSTEERLKVMNLAQDLAASRLTGTLMGFTINAAGS
                     PVTNQIVVRNLYDLEKRIQVAKEIVGMK"
     gene            complement(1783..>2361)
                     /locus_tag="VEL68_00330"
     CDS             complement(1783..>2361)
                     /locus_tag="VEL68_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HXZ34442.1"
                     /translation="LTREVVKAALVPVGVNALRNDAQSALAIATAAEAHFIRVNVHMN
                     AVVSEQGIIQGESHNTLRLRAALRSEVLIFADVGVKHAAPLADRGLAVETKDLADRGL
                     ADAIIVSGELTGSETKIEDVDIVRRNTDLPILIGSGATPENLHKVYSKVDGFIVGSYF
                     KKDGKADNLVDEKRVKAFTDALAKQRKGRRSP"
BASE COUNT          520 a          720 c          640 g          481 t
ORIGIN      
        1 aagggaatcg tccccggagc ccaggaaaag gaagtcatga aggccatggt cgccgaagga
       61 gacccaacca ataagctaaa accctaaccc acgcaccgcc ggccttattt cgttgttgga
      121 caggaatcct tccatagccg ccaagtttta cagacgcatg gctatgggca ttgcccagcg
      181 cttgaaatac atgaacgaag acttcgccgt cctgaaagct tttctttcca gtggaaaatg
      241 gagccctttt ttttaagtcc acaatccgaa atctgcaatc cgaaatccaa atggggaggc
      301 ttccatggcg cttttgaccg gcaaagaata cgtggagagt ctgaaagaac tttcccccga
      361 ggtgtatgtc cggggagaaa aggttcagga aatttggtcc catccccttc tccggcaaac
      421 cgtgaatcac atcgccgctg gatacgactt cgccctggac ccgagatacc gcgcatccgc
      481 tgccgttcaa tccccgctta ccggagagga agtaccgagg attcaacttc acatccagga
      541 aagcctggag gatgtggtcg ccaaggccaa attgacccgg gaaatcaccg gccgaagggt
      601 ctgcgccggg tgcatgtcca acatgctctc tgtgacctgg gccttgatct atgacatcga
      661 tcaggcccac agcaccaagt actttccccg tttccgggaa ttcgtcaaaa gcctccagaa
      721 gaaagaccag atcatcgcct ggggcatgat ggacccgaag ggagaccggt ccctcccccc
      781 ctcccagcag aaacattccc cggacctgag gattgtgaag caacgctcgg acggaatcct
      841 ggtccggggg accaaagtcc acaccacatt cggcccagcc gcccatcaca tcgtggtggt
      901 cccctgccgt gccctcggcc cggaagacaa ggaatacgct gtctcctttg ccctgcccat
      961 cgacgccaaa ggcctgaaga tgatcgcgcg cccttctccg gggccggcgg cagaaacgaa
     1021 aatggaaagt cctctaagct cccgctacct tggggtcgaa gccatgaccc tcttcgacga
     1081 tgtcttcgtt cccgaagata aaatcttcat gtgccgggaa tgggatcagg cgttcaagct
     1141 tcctctctac tttgcatccc tccaccggca gtctaagtgc gcctgcagcg cagggcacac
     1201 ggacctcttc atcggaacct cggctctggc cgcggaggtc aacggcctcg gcatgaaagt
     1261 ctctcacatc cgggacaaga tcacggagat gatgatggcc gctgagaccg ccttcggttg
     1321 ttccctggga gcagccctgg aaggaaagaa acatcccagc ggggtctttc tccctgaccc
     1381 ggtgatcgcc aattccggcc tgaacttcat ccgctcccgc atggggactc acctgagtta
     1441 tcttcatgac atcgccggcg ggcttttgag caccatgcct acggaagcgg actggaatca
     1501 tccggacctt cgaaagtaca ttgaggaggg cctgcgggga agcccgaagt actccacgga
     1561 ggagaggctc aaggtcatga acctggccca ggacctggcc gcttcccgtc tcaccggcac
     1621 cctgatgggg ttcaccatca acgccgcggg ctcgccggtg accaatcaga ttgtggtgcg
     1681 gaatctctat gatttggaga aaaggataca ggttgccaag gagatcgtgg ggatgaaata
     1741 aaggccgaaa ttctcaaaca caaaccatga atccgaataa acctaagggc ttcttcttcc
     1801 ctttctttgt ttggccaacg catcggtgaa cgccttcacc cgcttctcat cgacgaggtt
     1861 gtcggctttt ccgtcctttt tgaaatagct tcccacaatg aagccgtcca ctttcgaata
     1921 caccttgtga aggttctcgg gggtcgctcc gctgccgatc agaatgggga ggtccgtgtt
     1981 gcggcggacg atgtcaacgt cttctatctt tgtttccgag ccggtcagct cgcccgaaac
     2041 gatgatcgcg tccgccaggc ctcggtccgc caggtctttc gtctccacag ccagaccccg
     2101 gtcggcaagc ggggccgcat gcttgacccc cacgtcggca aaaatcagca cctccgaccg
     2161 gagggccgcc cgaagtcgga gcgtgttatg gctttccccc tggatgattc cctgttccga
     2221 caccacggca ttcatgtgga cgttgacccg gatgaagtgg gcctctgccg cggtggcgat
     2281 cgccagcgcg gactgggcgt cgttgcgcag ggcattgact ccaaccggga cgagcgcggc
     2341 tttgaccact tcccgggtga g
//
LOCUS       DASPRR010000008        16958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_968266, whole genome shotgun sequence.
ACCESSION   DASPRR010000008 DASPRR010000000
VERSION     DASPRR010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16958)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16958
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968266"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..581
                     /locus_tag="VEL68_00335"
                     /pseudo
     CDS             423..581
                     /locus_tag="VEL68_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            877..1611
                     /gene="tatC"
                     /locus_tag="VEL68_00340"
     CDS             877..1611
                     /gene="tatC"
                     /locus_tag="VEL68_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXZ34443.1"
                     /translation="MEELRKRLIYSFTGIGIAFALCYAYIKEIVEILMRPLVKTLPPG
                     SSLVFTAVPEAFFTYFKAAILAGVFFSSPFLLYQLWAFVSPGLYRREKKYILPYLLFS
                     SFFFLLGAVFCYFVVFPTIFRFFLSFATDSIRPLPSIREYLTFTIKLLLAFGLLFQWP
                     PLVFFLARMGVISGSFLSQNRKYAILIIFVAAAVLTPPDLVSQLLLAGPLIVMYEGSI
                     WMAKIFGKKEKKEEDQADPAAGGKQG"
     gene            complement(1652..2752)
                     /gene="dnaJ"
                     /locus_tag="VEL68_00345"
     CDS             complement(1652..2752)
                     /gene="dnaJ"
                     /locus_tag="VEL68_00345"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXZ34444.1"
                     /translation="MIKKDYYDLLGISSSASLDEIKRAYRRLAHRCHPDKNPNNPSAE
                     EQFRKITEAYEVLQDADKRAVYDRQVPSPGKRGFGGGRPSPGFSGPKDVFDGIFEEIL
                     EDFFGTEGSKAKKKKGADLRYGLEISLEEAAFGSQKKVQFNRQSICPLCRGSRCAPGT
                     EPELCPYCRGTGSLQSHRGFFVVETACDRCGGEGEYIPRPCPRCGGGGQIKTPQAFTI
                     QTPPGAEKDTRLRIAGEGGMGQNGGPPGDLFVVLSVRQHPIFVRLGNDLQVEIPVPLI
                     QAFRGAEIHVPVLNGTVRMKVPPRTPPGKVFILKGLGMPVFQKKGKGDLRVKIRVEAP
                     GHRRKQDREMLEELARQSKMGKTGLETASFTR"
     gene            complement(2749..3318)
                     /gene="grpE"
                     /locus_tag="VEL68_00350"
     CDS             complement(2749..3318)
                     /gene="grpE"
                     /locus_tag="VEL68_00350"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXZ34445.1"
                     /translation="MKIERKKEEKVTQEPENLPERKEGLSPEKLEVQLEEKTREASEY
                     FDKWLRLRAEFENFKKRMQKEKGDLVKFGNENLLKALLPVLDNLSRAIEHGKNAKENS
                     PLLGGVEMTYKEFLNILDRFGIKPVPAVGEVFDPEKHEALSQEESDQETNRVIAAVQT
                     GYFYHDRLLRPAKVIVSKGKSEAGKETKE"
     gene            complement(3436..4476)
                     /gene="hrcA"
                     /locus_tag="VEL68_00355"
     CDS             complement(3436..4476)
                     /gene="hrcA"
                     /locus_tag="VEL68_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXZ34446.1"
                     /translation="MYEGFTERDRKVLQAIIRDYIQTAQPVGSRIISKKYQMGLSPAT
                     IRNVMADLEEMGFLLQPHTSAGRVPTDRAYRFYVDTILNLRRLTPEERAQIETSLIPE
                     EVQDVNEIMKRASHLLSLLSRQTGVVLAPRFGSKIFKHIEFITMREKRILVIIVSQTG
                     EVQNKIIEADEEISQDELDRYSKYLTEIMGGLSLAEAKNRIMEELKNEKVLFDKLMFR
                     ALQLSQKALEDEGEGNLYIDGKTNIMQSPEFADMEKIQSLLQTFEEKTKIVKLLDKAL
                     SVQGIQIFIGAENELNEMVNCSVVTAPYSRENYTLGTLGVIGPTRMDYSSIIPIVDYT
                     ARILGRILENID"
     gene            4780..5556
                     /locus_tag="VEL68_00360"
     CDS             4780..5556
                     /locus_tag="VEL68_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ34447.1"
                     /translation="MLESLFSLKGKVALITGGSRGIGRAIALAYAEAGADLVISSRNR
                     RPPELQKVAEEIQAVGKKALPVPAHVGRKEELESLVQKTLEAFGRIDILVNNAGANPV
                     LSAMIDLEEDSFEKVLEVNLKGAFLLSKAVAREMIKKGSGGRIINMSSVSGLRARDDG
                     TGAYCISKAAMNMMTQVMARELAPYNILVNAIAPGSIKTDFSRVNWTDPERKAQRIRE
                     IEIKRFGEPEEVVGLALFLASAASSFVTGEIIRVDGGQTI"
     gene            5660..6433
                     /locus_tag="VEL68_00365"
     CDS             5660..6433
                     /locus_tag="VEL68_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXZ34448.1"
                     /translation="MANPTYEIYAVKYGGPYIRPACMVNWFQDMDKTTRINYYIFVIR
                     GGGETIVVDCGVDPQLAKQRNLADYVNPVDALKRMDIDAARVKHLIVTHVHFDHVSGI
                     QLFPKADIYIQEKEFTFWVKNPIAQRAPFLFVTDSVANRYLARQEGKKRLRLIRGDKK
                     ILPGIELLLAPGHTVGLQAVAVHTAKGEAIVGSDLAHTFASYQTDIPSAIITDLVVWM
                     KSFDKAKAKASSPELLFPGHDLGLMEHYPKVAKDVTRLV"
     gene            complement(6528..7097)
                     /locus_tag="VEL68_00370"
     CDS             complement(6528..7097)
                     /locus_tag="VEL68_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ34449.1"
                     /translation="MKVLGVLGSPRVGGNSDILLDQALAGAKGAGAEVDKILLCQAKI
                     SGCLDCTKCNEAGVCAIQDDMQTLYPKILEADAIIHSVPVYFWAMTAQMKAYLDRWCA
                     FFDAEWKWHKHYYSKMKGKKIGLITVCGDSNVHTADPIVHSFKTTAEFTKLKWIGAVM
                     ASAAAKGEIGKNEKSKKEAYDLGKKAAGK"
     gene            7628..8203
                     /locus_tag="VEL68_00375"
     CDS             7628..8203
                     /locus_tag="VEL68_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34450.1"
                     /translation="MESEDLTKTLETLKAISDLELAIAELYRCFSEFRASEKEFWTAL
                     GEDEGRHARMIQKMVQMLLDRKFFCAPNGSFNESAVTNLKNFVERHQRKLQKLEIPTD
                     FMSLLSIAWNVEYSLSEMNYTNLFSITEGELESLLLTVTSETAVHRSKIGSKITVMRN
                     SLPRSHHRGTPKGNPGVQRKVNSKAHIPKFT"
     gene            8330..9517
                     /locus_tag="VEL68_00380"
     CDS             8330..9517
                     /locus_tag="VEL68_00380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ34451.1"
                     /translation="MPPNRNTSSSFPVLPLFLLVFIFYLNFVSRVIMAPLLPIVEAEL
                     GFGHGQSGSLFFFVAFGYGVGLLGSGFTSYLLTHRFTITLAGMMGGISLVMISHSHSI
                     GEIHAGLVLIGIFAGLYLPSGIASLTELITKEHWGKAMAIHELAPNAAFITAPLLAEF
                     LLKFLSWRGVLNVLGVWAILMPVLFLIFGRGSRKRGEAPRLRLIREVLGNPFCWVMGL
                     FFMIAIGSSLGVYAVMPLFLVTEMGMDRPWANTLIGLSRSFGVVVLFISGIMIDRIGP
                     IRALTTFMITTGLFTLLIGTIPGSTPVSILVFFQAAFVTCLFPTGFTIVSLIFPERLR
                     GTAVSLVIFIGFLFGGGVVPSALGHWAEVFSFASGFALLGIVTLIMLPFFRRVTFRLN
                     LSR"
     gene            complement(9839..10537)
                     /locus_tag="VEL68_00385"
     CDS             complement(9839..10537)
                     /locus_tag="VEL68_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXZ34452.1"
                     /translation="MAANLRKEDLEALRKWPTCAISNAIELFNIQPRNVGFMLPEIKC
                     QFPDMGNMVGYAVTAVITADTSEGRRVPPPEWWEMIKKTPEPRVAVIHDLDRPVIGSF
                     WGEVNANIHKALGCAGVVTDGSVRDMDEVRELGFQFYSSCVTVSHAYVHLVEIGAPVK
                     VGGLLVKPGDLLMGDKHGVIKVPLEIAKDVPKAAQMMEDWERKVINFCKSKEFNTEGL
                     KALFMSPRPTWPSK"
     gene            complement(10726..11922)
                     /locus_tag="VEL68_00390"
     CDS             complement(10726..11922)
                     /locus_tag="VEL68_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXZ34453.1"
                     /translation="MKERGFFLMKEKDLARLVKEDEVVKMSQDLVRTQSVNPPGNELA
                     AAEYVVEALKKSGVEAELVKHSDTRASALARLKGQGKVPALFYNGHLDTVPVGTEKWI
                     HEPFGGEVAEGKIWGRGAADMKSGLAAMMAAMKVLAEARLPLKGDLILAATAGEEIDS
                     LGATAVAKMLSQEPIQALFIAEPTYNEIYIAEKGVFWVQVDTFGKTAHGSMPEKGRNA
                     VMMMVKIINELEKVEIPYKPHPILGGFSRSINTVSGGIKTNVVPDHCVATIDMRTVPG
                     QDHSAILRRIENVIKDLSQKIPDFKATVKAVNDHVSVETSPSDPIVQKFSAVVTEMTG
                     QKGVLKGANYFSDAVGFLPILKLPLILFGPGEPGQAHQPNEHVEVSRLVEAARIFAVA
                     AARLLG"
     gene            12334..13647
                     /gene="trmFO"
                     /locus_tag="VEL68_00395"
     CDS             12334..13647
                     /gene="trmFO"
                     /locus_tag="VEL68_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXZ34454.1"
                     /translation="MKQGLTIIGGGLAGCEAAWQAARREIPVLLYEMKPLQFSPAHVS
                     PYLAELVCSNSLRSNSLENAVGLLKEELRRLGSLLMAVAEKTRVPAGDALAVDRQAFA
                     AAITDRLQKMEGMSIVRDEVTEIPREGTVIIASGPLTSSDLAESIQKVTGAEYLYFYD
                     AIAPIVDGDSLGMEVIFRASRYGEGSDYLNCPMSPEEYFRFVEELSKGEKVAYREFEK
                     PLHFEGCLPVEEMAERGRETLSHGPMKPVGLKDPRTGKTPYAVVQLRKEDNRGSAYNL
                     VGFQTKLKWKEQERVFRMIPGLGKAVFLRFGSLHRNTFIHSPTLLEKTLQFKKDPRIF
                     FAGQLTGVEGYVESTAMGLLAGINAARMARGKTPAFPPPTTSIGSLVHYLTDARVKEF
                     QPMNVNFGLFPPLEGRIKGREKKRAMGERALKDLGEWIQEIGERI"
     gene            13644..14171
                     /locus_tag="VEL68_00400"
     CDS             13644..14171
                     /locus_tag="VEL68_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ34455.1"
                     /translation="MISKSFPHKADVVVVGAGPAGSILAYQLAKAGVDVVLLDKARFP
                     RGKTCGGGINVRTQKQIPFDFSPIIEGTITGISFTCKFEDPFIRRYTAPLMFTVRREH
                     FDEFLARRAEEAGARFFDQTPFLSVDQKGGYGSGNRGGGMLGGVRCRGGRCAKPGCQE
                     NAPLFRPGLHPGHSQ"
     gene            14125..14799
                     /locus_tag="VEL68_00405"
     CDS             14125..14799
                     /locus_tag="VEL68_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34456.1"
                     /translation="MPLFSDPAYILAIHSEAPASLIPDWDPALIHIDWGSVKRSYAYM
                     FPKKYFLSAGAGGVDVPAGKLKNYHRAFLATRSQKDQALPFSAAGFMIPLRKNRSPIH
                     SGRCLLVGDAAGLADPFSGEGIFSAVRSAQIASSALEEALKGNCDSLKLYEEAIDRDL
                     MPELECSRLFRAFFNLRPSHYHRKIASQDRWWNAMAKIMRGEKTFLDIKKKLGPVGSL
                     LLRMAR"
     gene            complement(14887..16044)
                     /locus_tag="VEL68_00410"
     CDS             complement(14887..16044)
                     /locus_tag="VEL68_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ34457.1"
                     /translation="MGEKFLLTPFGEEHEVFRQQCRRFVEKELAPHVMEWEKKQDFPD
                     EVFRRVGEMGLHGILIPEEYGGSGSDYLMASVWVEESARIRSGGVEAGIGMHGLIVLP
                     AIAKFGNEDQKKRFLPPGVKGEKIGALGLTEPIAGSDLANIRTVAKREGDHYLLNGSK
                     TFITNGSRADFVLVLCKTSPEAGYKGFSTLIVEKGMAGFTQGRPLEKLGWHAGDTAEL
                     TFENVAVPAGNRLGEEGSGFYNSMANLEWERLIMALGTVCSAQLIFDRTKTYVLQREA
                     FGRPIGKFQVNRHKMVDMAMEIEAARQLNYHALRKLMNKEPCAIEVSMAKVFASRMGE
                     FVATTCLQLHGGYGYMREYEIERFFRDVRINAIGGGTSEILKEIVGRLMGF"
     gene            complement(16052..16822)
                     /locus_tag="VEL68_00415"
     CDS             complement(16052..16822)
                     /locus_tag="VEL68_00415"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXZ34458.1"
                     /translation="MLLVIDVGNTNTVLGIYEGARLVDSWRIWTERDRTSDEYGILMR
                     NLFSSRAIPLENIGAVSISCVVPPMLNMLLELCEKYFHVEPLVVDVGIQLGMPILTDT
                     PAEVGADRIVNAVAAYHKYGRSLIVVDFGTATTFDYISPQGEYMGGAIAPGLGIASEA
                     LFIRASKLPRVELKRPKRVVGKNTVQSMQAGIIFGYVGLTDEIVLRMKEEVKTNPKVI
                     ATGGLAPLIASESKTIEEVDEFLTLEGLRLLYELNRKR"
BASE COUNT         3640 a         4876 c         4342 g         4100 t
ORIGIN      
        1 gaggcgggcc catcgggcgc ccagtaaggc ggaacgcgtg acgcccctac atcgggatag
       61 ccctgttctt agggaaagaa cggcttgccc gcccggtcca agaaatgggg tgtgcctcat
      121 ggtttttgcg cgcaacgccc ttgctttcgc gccgggcaat gcctgaaatt caatcgatca
      181 tacgcctact tgccaaaaag tgaaggtggg caaggttctt atcggatggg gaatgcaaat
      241 tcctgagaag taacctgccg gttcccggtt actcgttgcc gccacctgcc cggggattgg
      301 gcctcaaacg ctgaaccaga aacctttttg aatctggccg ccgagctcac aattccaaac
      361 tgttttttct tattgacacc cctcctaaaa ctttctattc tggaaatgaa agtgagttcg
      421 agatgtttgg cattggcctt ccagagctgg tcattattct catcgttgcc ttgatcgtgt
      481 ttggcccccg gaagctcccg gacctggcca aatccctggg aaaaggcctg gccgaattca
      541 agaaagccag cgatgatctt aaatccagca tcgaaagcga cctgcgggtt gatttaaacg
      601 aagagcctaa taagtccgag gctcccccgc aggccgagtc cccggttccc aatcctcccg
      661 attcagagac cccgccggca gagccacttc cgccccggga ggcattcctt gaggatttgg
      721 agagggggaa acacgaggtg gtgcaaatct ccgaagacct tcagccccca gaaccctcgg
      781 ctccggtgac tcccccgcag aaggaagaaa atcaacctgt cgccccccaa gaagccaaac
      841 gacatatcgg ataagaaact ccctctctct gcacatctgg aagagctgag gaagaggctt
      901 atctattcct tcaccggaat cgggatcgct tttgcgctct gctatgctta cataaaggaa
      961 atcgtggaga ttttgatgcg tcctctggtg aagactctgc ccccgggaag ctccctggtc
     1021 ttcaccgctg ttcccgaggc tttttttacc tatttcaagg ccgccatcct ggcgggcgtt
     1081 ttcttctcct cgccatttct gctctatcag ctctgggctt tcgtgtcgcc ggggctctac
     1141 cgacgggaga agaaatatat ccttccgtac ctgcttttct cctccttttt tttcctcctg
     1201 ggagcggtct tctgctattt cgtcgtcttt cccacgattt tccgtttctt ccttagcttt
     1261 gccaccgaca gcatccgccc tctcccttcc attcgggagt atctaacctt caccatcaaa
     1321 ttacttctgg cttttggcct cctcttccag tggccgcccc tggttttttt tctcgcccga
     1381 atgggagtga tttcggggtc ctttctttcc cagaaccgaa aatacgccat cctgatcatc
     1441 tttgtcgccg ccgctgtgtt gactcccccg gacctggtct cccagcttct cctggcaggt
     1501 ccgctcatcg ttatgtatga aggttctatc tggatggcca aaattttcgg gaagaaagaa
     1561 aagaaggagg aggatcaagc ggatcccgct gccgggggaa agcaggggta aagcttggag
     1621 ggttgttttg aggaaaaagg ggcctccgtg ctcaccgggt gaaggaggct gtttccagcc
     1681 cggtttttcc catcttgctc tgtcgggcca gttcttccag catctcccga tcctgtttcc
     1741 tacgatggcc aggagcttcc acccggattt tgacccttag gtcccccttt cccttctttt
     1801 ggaagactgg cattcctaat cctttgagga tgaaaacttt gcccggagga gtcctgggag
     1861 gcaccttcat ccggacggtc ccgttcagga cgggaacatg gatttctgcg ccccggaagg
     1921 cttggataag ggggacagga atttcgacct gaagatcgtt ccccagccgg acaaagatgg
     1981 gatgttgccg gaccgacagg acgacgaaaa ggtcgccagg cgggccgccg ttctggccca
     2041 tccccccttc tccggctatc cgcaggcggg tgtccttctc cgcacccggg ggagtttgga
     2101 tggtgaaggc ctggggagtt tttatttgac cgccccctcc gcaccgtgga cacggccggg
     2161 ggatgtattc cccttctccc ccgcagcggt cgcaggccgt ttccacgaca aaaaatcctc
     2221 gatggctttg aagggacccc gtgcctcggc agtagggaca aagctccggc tccgtccccg
     2281 gagcacaacg actccccctg cagagcgggc agatcgactg gcggttgaat tggaccttct
     2341 tttgggaacc aaaagctgct tcctccaggg aaatttccaa gccgtaacga aggtctgccc
     2401 cctttttttt cttcgccttt gagccctcgg tcccaaaaaa atcctccaga atctcctcga
     2461 aaattccgtc gaaaacatct ttcggcccgg agaagccggg cgatggccgc ccccccccga
     2521 atcctctctt gcccggggaa ggcacttggc ggtcgtacac cgcccgctta tccgcatcct
     2581 gcaggacctc atacgcctcg gtgatctttc taaactgctc ttccgcggag gggttgttag
     2641 gattcttatc gggatggcaa cgatgggcta ggcgacggta agctctcttg atctcatcca
     2701 ggctggccga ggagctgatc cccaggagat cgtagtaatc ctttttgatc attccttggt
     2761 ttctttccca gcctccgact ttcccttgga gacgatcacc tttgccggac ggaggagccg
     2821 gtcatggtaa aaatatccgg tttgaacggc ggcaataacc cggttggttt cctggtcact
     2881 ttcttcttgg gaaagggcct cgtgcttttc tgggtcgaag acctccccca cggccggcac
     2941 cggttttatc ccaaaccgat ccaggatgtt aaggaactct ttgtaggtca tctccactcc
     3001 tcccaggagg ggagagttct ccttggcatt tttcccgtgc tcgatggccc gggataagtt
     3061 gtccaggaca gggagcaggg ccttcagaag gttttcgttg ccgaacttca ccaggtctcc
     3121 tttctccttc tgcattctct tcttgaaatt ttcaaactct gccctcaaac gaagccattt
     3181 atcaaaatat tccgaagcct cccgggtttt ctcttccagc tgaacttcaa gtttttcagg
     3241 agaaagtcct tcctttctct ccggcagatt ttccggctct tgcgtgacct tctcttcctt
     3301 ctttctctcg attttcacct ctaccagatc ttctccaggg ttcttttttc cgctatgact
     3361 ctttttttcc aaggcacctc tccccttctt aaaaatataa aattacgggt ctggcagctc
     3421 cggatcccga tggactcaat cgatattttc caggattctc ccaaggatac gggcggtata
     3481 atcgacgata ggaatgatgc tagaatagtc catccgggta ggcccgatga cccccagggt
     3541 ccccagagtg taattttccc gggaataggg ggcggtcacg acgctgcaat tcaccatttc
     3601 gttcaattcg ttttccgcac cgatgaagat ctgaatcccc tgaacggaaa gggccttatc
     3661 caggagtttg acgatcttgg ttttctcttc aaaggtctgg agaaggctct gaattttctc
     3721 catgtcggca aattcagggg attgcatgat gttcgtcttg ccgtcgatgt acaagttgcc
     3781 ttccccctcg tcttcgagag ccttctgact gagctgaagg gcccggaaca tgagtttatc
     3841 gaataaaacc ttctcgtttt tcaactcctc cataatccgg tttttggctt ccgctaggct
     3901 gagtcccccc atgatctccg tgaggtactt gctgtatctg tcgagctcat cctggctgat
     3961 ttcttcgtcg gcttcgatga tcttgttctg tacctctccg gtttgagata caatgatgac
     4021 caggatcctt ttctccctca tagtgatgaa ttcgatatgt ttgaagattt tactgccgaa
     4081 acggggagcc agaacgactc ccgtttggcg ggagagaagg gagagcagat gggaagcccg
     4141 tttcatgatt tcattgacat cctggacctc ttccggaatc agactggtct cgatttgtgc
     4201 tctttcctca ggggtcagcc ttcgcaggtt caaaatggta tccacgtaga aacgataggc
     4261 ccggtctgtt ggaacccttc ctgccgaggt atggggctgc agcaaaaatc ccatctcttc
     4321 caggtcagcc atgacattgc ggattgtggc tgggctgagc cccatttggt attttttgga
     4381 gatgatccgc gaccctaccg gctgcgccgt ctggatgtaa tctcgaataa tcgcttgaag
     4441 gaccttccgg tcgcgctccg taaagccctc gtacacagat aacctctcaa cactcttaat
     4501 aaataagtgt attttaaaaa taacaataaa tacccctctt gtcaaggaca gggccggttt
     4561 tgcccccctt ctcttttaag atgccctttc ttgctgcccg gggacaaggt accgcctctg
     4621 gcgggatagg gtcggcgaaa aggcgtgatg aaaaacctca tcccttccat attttgaaga
     4681 gtttgcccat ggatggtgca gagaacaatg agctatggaa aaaggttgcc taaatttgaa
     4741 tttgggttaa aaatagtctt caaggaggcg tgggtcatta tgctcgaaag cctgttttct
     4801 ttaaagggaa aagtagccct catcactggg ggaagtcgtg ggattggaag agcgatagcc
     4861 ctggcttatg ccgaggccgg agcagacctg gtcatatcca gtcgaaatag acgccctccg
     4921 gaactgcaaa aagtggctga agagattcaa gccgtgggca agaaggccct tcccgttccc
     4981 gcccatgtgg ggagaaagga agaacttgag agtctggttc agaagaccct cgaggcattc
     5041 gggagaatcg acatcctggt gaacaacgcc ggggccaatc cggtcctgag cgccatgatc
     5101 gacctggaag aggattcctt tgaaaaagtc ctggaggtta acctgaaagg agcctttctc
     5161 ttgagcaagg ccgtggcccg ggaaatgatc aagaagggga gcgggggcag gatcatcaac
     5221 atgagttccg tcagcgggct ccgggcccgg gacgacggca ccggggccta ctgcatcagc
     5281 aaggcggcca tgaacatgat gacccaggtg atggcccggg agcttgcccc ctacaatatc
     5341 ctggtgaacg ccatcgctcc gggttcgatc aaaactgact tcagccgggt gaactggacc
     5401 gaccctgagc gcaaggccca gcggatccgg gagatcgaga tcaagcgctt tggcgagccc
     5461 gaggaagtag tgggtcttgc cttatttttg gcttcagcgg caagttcctt cgtaaccgga
     5521 gagatcatcc gggtggacgg aggacagacg atatgagaaa tcaatgctca atgatcaatg
     5581 accaatgggc aataaccagg gaacaacgaa tccttttctt cgcgcgctgc ttttgaaaaa
     5641 tctgaaaggg ggaaagatta tggccaatcc gacctatgaa atctacgcag tgaagtacgg
     5701 cggcccttat attcgtccgg cctgcatggt taactggttt caggatatgg acaagacgac
     5761 ccggatcaat tactacattt tcgtcatccg gggaggcggg gagacgatcg ttgtcgattg
     5821 cggtgtcgac cctcagctgg ccaaacagcg caacctggcc gattatgtga accccgtaga
     5881 cgcccttaag agaatggaca ttgatgcggc cagggtgaag cacctgatcg tgacccatgt
     5941 ccacttcgac catgtcagcg gcattcagct ttttcccaag gccgatattt acattcagga
     6001 aaaggagttc accttctggg tgaaaaatcc cattgcccag agggcgcctt tcctttttgt
     6061 cacggactct gttgccaacc ggtacctggc ccggcaggaa gggaaaaaga ggttacgcct
     6121 catccggggg gataagaaaa tactccccgg gatcgagctg cttcttgccc ccggccacac
     6181 cgtcggcctc caggccgtgg ccgtccacac ggccaagggg gaagcaatcg tgggatcgga
     6241 cctggcccac acttttgcca gttaccaaac cgacatcccc agcgccatca tcaccgacct
     6301 ggttgtctgg atgaagagtt ttgataaggc caaagccaaa gcctcctctc ccgagctgct
     6361 ttttccgggc catgatttgg ggctgatgga acattacccg aaggtagcca aagacgtaac
     6421 ccgattggtc taggacagga atgaagggct aggatcaagg ccgaaggaaa gccgcgcagg
     6481 aacgattcca ggaaacaccc tcgaggcttg ttccccgttc ctgctcctta ttttccggct
     6541 gccttctttc ccagatcata ggcttctttt tttgatttct cattttttcc aatctcgccc
     6601 ttcgctgcgg ccgaggccat taccgctccg atccacttga gcttggtaaa ttcagcggta
     6661 gtcttgaagc tatgcacgat agggtcggcg gtatgcacgt tgctgtctcc gcagaccgtg
     6721 atcaggccga tctttttgcc cttcatcttg gagtaataat gtttgtgcca tttccattcg
     6781 gcgtcgaaga aggcgcacca gcggtccagg taggccttca tctgcgcggt catggcccag
     6841 aaatagaccg gcacgctgtg aatgatggca tccgcttcga ggattttcgg atagagggtc
     6901 tgcatatcat cctggatggc gcagaccccg gcttcgttgc acttggtgca atctaaacac
     6961 ccggagattt tcgcctgaca aaggagaatc ttgtcgacct ccgcccctgc tcctttcgcc
     7021 ccggcgagcg cctgatccaa aagaatatcg ctgttccccc cgacccgcgg actccccaga
     7081 actcccaaca ctttcatttt tccctccttt cccatccccc aaattgcctc cggctttttc
     7141 ccgaaggctg cttgccaaaa ctccacacat tccactgcca caacggcaga atgattcaat
     7201 gtatattacc agagggaatt gacaaaaaga agaggaagga agggccgggc ctgaatcgag
     7261 ggaggtgaat aaaaaaacaa cgatcttcgc agccaagaca tatctcttca aaaagaatct
     7321 ttgcgggggg aaagaggaga atacctccaa acaccccgat taccttccat atccccggct
     7381 tcatttcctg agtgatcatc caaaatagcc cggttttcat ttctacctca atcttcttcc
     7441 tttttaaaat ttttccccaa cccgaagaaa ggggaaattt ctcccgaatc caaaaacaaa
     7501 acttctttca ttttggaagg aacggaccga taagtataat agtcagtagc cccccacatc
     7561 ctttagccga atgctgcaac ggaaatcgca cgatttcgtt ttttgatcct gggcagggaa
     7621 gaatgagatg gagtcagagg accttaccaa aactctggaa accctgaagg ccatttccga
     7681 tttggagttg gccattgccg aattataccg ctgcttttcc gaattccggg cttcggagaa
     7741 agaattctgg accgccttgg gagaggatga aggaaggcat gcccgaatga ttcagaaaat
     7801 ggtccaaatg cttctggatc gaaaattttt ctgcgctccc aatggttctt tcaatgaatc
     7861 ggcggtcacc aacctgaaga attttgtgga gagacaccaa cgcaagctcc agaagctcga
     7921 aatccccacc gatttcatgt cccttctctc catcgcctgg aacgtggagt attccctttc
     7981 cgaaatgaac tacactaacc tcttctccat cactgaggga gagttggaat ctttgctgct
     8041 gaccgtcact tcggaaaccg ccgtccatcg aagcaagatc ggatcaaaga tcacggtcat
     8101 gcgcaacagc cttccccgct cacaccatcg cgggaccccc aaaggaaacc ccggggtcca
     8161 gaggaaggtc aactccaaag cccatatccc taaatttacc tgaggcaaac ccgtctcttt
     8221 tccacatctg tccttcttct ttgacaccga tggccttttg gtgttacaag aggagaaatc
     8281 gccctattca accagagagg agggagattt tttaaccagc acttcggata tgcctcccaa
     8341 caggaataca tcctcttctt tcccggtctt gcctctgttc ctcctggttt tcatctttta
     8401 cttgaatttc gtttcccggg tgatcatggc ccccctcctg cctatcgtcg aagcggaatt
     8461 aggcttcggc catggccaat cagggtctct tttctttttt gtggccttcg ggtatggcgt
     8521 cgggctcttg gggtccgggt tcacttccta tcttttgacc catcgcttta ccatcaccct
     8581 agcggggatg atggggggga tttctctggt catgatttcc cattcccatt cgatcggcga
     8641 aatccatgcc ggtctggtcc tcatcgggat ttttgccgga ctttaccttc cctcgggaat
     8701 cgccagcctc accgagctca tcaccaagga gcattgggga aaagcaatgg ccatccacga
     8761 attggccccc aacgcagcct ttatcaccgc ccccctcctt gctgaattcc tgttgaaatt
     8821 cctttcctgg cggggagtac taaatgtatt gggagtttgg gcgattctca tgccggtcct
     8881 gttccttatt ttcgggcggg gaagccgaaa acggggagaa gcgccccggc ttcgattgat
     8941 ccgggaagta ctcggcaatc ccttttgctg ggtcatgggt ctcttcttta tgatcgccat
     9001 cgggtccagc ttgggcgttt acgcggtgat gccccttttc ttagtcacgg agatggggat
     9061 ggatcgccca tgggccaata ccctgatcgg cctttcccgc agttttgggg tcgtggtgct
     9121 tttcatctcc ggaatcatga tcgaccgaat cgggcccatc cgcgccctta cgaccttcat
     9181 gatcaccacc ggccttttca cccttttgat tggaaccatt ccaggctcca ccccggtttc
     9241 catcctggtc ttcttccagg ccgcttttgt cacctgccta ttccccacgg gatttacgat
     9301 tgtttccctg atttttccgg aacggcttag gggtacggcg gtttccctgg ttatcttcat
     9361 cggcttttta tttggcgggg gggttgttcc ttccgccctg gggcattggg ccgaagtctt
     9421 ttctttcgcc tcgggttttg cccttctggg gatcgtcact ctgatcatgc ttcccttctt
     9481 ccgccgcgtc accttccgcc tgaatctttc ccggtagcga aaatcagaaa tgggctttcc
     9541 catttaaccc atgttgacat ttccagcccg aaaaccccct ctccccttcg tgggagaggg
     9601 tcggggtgag ggggattgaa gcagagaagc aggagattcc gaagactttt cacccccacc
     9661 cttccctccc ccatcgaggg ggagggagaa tcaacgctga agaacctcaa gggtcaagcg
     9721 gaaaatcgtt ctttgtatca gccaaccgta tccgccggat ttttgaaaag tgggccgaac
     9781 gaacatgagt gaagtgaaaa agcccaatca gaaatttgat ttgatattcc aagccctcct
     9841 atttggaggg ccatgtcggc ctgggagaca taaagagcgc tttcaaccct tccgtattga
     9901 actccttgga cttgcagaag ttgatcactt tccgttccca atcctccatc atctgggcgg
     9961 ctttgggaac atctttggcg atctccaggg ggactttaat gactccgtgc ttgtccccca
    10021 tgagcaagtc accgggcttt accaaaagcc cccccacttt gaccggagca ccgatttcaa
    10081 ccaggtgaac gtaggcatgg gacacggtga cacagctgga gtagaactga aaacccagtt
    10141 ccctcacctc atccatgtcc cgcacggagc catcggttac cacaccggcg caccccagag
    10201 ctttgtggat attggcattg acttcccccc agaaagagcc gatcaccggt cgatctaagt
    10261 catggattac agcgaccctg ggttcagggg tcttcttgat catttcccac cattccggcg
    10321 gcgggacccg gcgcccctcc gaagtgtccg cggtgatcac cgctgtaacc gcgtacccga
    10381 ccatgtttcc catgtccgga aactggcact tgatctccgg gagcatgaac cccacgttcc
    10441 gcggctgaat gttgaaaagc tcaatggcat tggaaatggc gcaggtaggc catttccgca
    10501 gcgcttccag gtcttctttc ctgagattgg cggccatttt cgaatccttc ctttccttag
    10561 gcaaagatgt ttgtaatgaa tcctcttgga aaacccaatt aaaagagagg tcccgaatca
    10621 ataagaacca aatccccttg gacccccctt gaacaacggg ggttgggggg atttccgtcc
    10681 accttctatc gacttggaat aagtttcgca gcgggaatca atggcttacc ccagcagcct
    10741 cgcagctgcc acggcaaaaa tccttgccgc ttcaaccagc ctggaaactt ccacgtgctc
    10801 gttcggctga tgggcctgcc cgggctcgcc gggcccgaag agaattaggg gaagcttgag
    10861 aatcgggaga aaccccaccg catccgaaaa ataatttgcc cccttaagaa cccccttctg
    10921 cccggtcatc tcggttacga cggcggagaa tttctgtacg attgggtccg aaggcgaggt
    10981 ttctaccgaa acatgatcat tgaccgcctt aaccgtcgct ttaaaatcag gaatcttttg
    11041 gcttaggtct ttgatgacat tttctatccg gcgcaaaatc gccgaatggt cctgcccggg
    11101 gacggtcctc atatcgatgg tcgcaacaca gtgatcgggg accacgttgg ttttgattcc
    11161 ccccgaaacg gtgttaatgc tccgggagaa tccgcccaag atcggatgag gcttgtaggg
    11221 aatttccact ttttcgagct cgttgatgat cttgaccatc atcatgactg cgtttcgacc
    11281 cttttccggc atggagccat gcgcggtctt gccgaaagta tcaacctgca cccagaagac
    11341 ccctttttcg gcgatgtaaa tctcattgta agtcggctcc gcaataaaca aggcctgaat
    11401 cggttcctgg ctcagcattt ttgcgaccgc cgtggcgccc agggaatcga tttcctctcc
    11461 tgctgttgcc gccaggataa ggtctccctt cagaggaagc cgggcttcag ccaaaacttt
    11521 catcgccgcc atcatggccg ccaaaccgct tttcatatcc gccgcccctc ggccccaaat
    11581 tttgccctct gccacctcgc cgccgaaggg ttcatgaatc cacttttccg tgcccacggg
    11641 aaccgtgtcc aggtgcccgt tgtaaaaaag agccggcacc ttgccctgcc ccttcaaccg
    11701 agccagggcg ctcgcccggg tatcgctgtg cttcaccagc tcagcctcca ccccgctctt
    11761 tttcagagcc tcaaccacgt actccgccgc cgccagctca ttcccgggcg ggttcacgct
    11821 ctgggtccgc actaaatcct gactcatttt aactacttca tcttccttca ccaacctcgc
    11881 caggtccttc tccttcatga gaaagaatcc tctttctttc atcccttttt ccgatcatga
    11941 ccgtcccaaa atattatagg aacccccatc gaaagtaaat acccgagcca aatatttctt
    12001 gccgaaatag aaagaacggg aaatatgcgg gaaatttcgg ggggtaatca tgtttccgtt
    12061 tcaatccccc caggacaggc ccgatccaat ggaagaacca ccgcctcagg ataatgatcc
    12121 aaatttctcc gccctgaacc ccatgagatc ccctctcctt tccagagatc ttattttcga
    12181 aggactcttt tttaagaact gggggtgagc aaggcaattc gttccttgct tccttgtcga
    12241 ccttgaggac ccattaaagc cttgacactg aagggcaata atgaaaaaat tcgatgggtt
    12301 gagaaataag cagaccggtt ttttgggaag accatgaaac agggattaac gattattggg
    12361 ggtggactgg ctgggtgtga agccgcttgg caagcggccc ggagagagat acccgttctt
    12421 ctttacgaga tgaagccact acagttctct cctgcccatg tttcccctta tctcgccgag
    12481 cttgtttgca gcaactccct tcgctctaat tccttggaaa atgccgtagg attactgaaa
    12541 gaagagttga ggcggcttgg ttctttgctc atggccgtgg cggaaaaaac ccgggtgcct
    12601 gccggcgacg ccctggctgt ggaccgtcag gcatttgctg ctgcgatcac cgatcggctc
    12661 cagaagatgg agggtatgag catcgtccgg gacgaggtaa ctgaaattcc cagagaaggg
    12721 acggtgatca tcgcctccgg ccctctcacc tcttccgacc tggccgaatc gatccaaaag
    12781 gtaaccgggg cggaatacct ctacttttac gatgccatag cccccatcgt ggatggggac
    12841 agcctgggca tggaggtgat cttccgtgcc tcccggtatg gggaggggag cgattatctg
    12901 aactgcccca tgagccccga ggaatacttc cgatttgtgg aagagctgag caaaggggaa
    12961 aaagttgctt atcgtgaatt tgaaaagccc cttcatttcg aaggctgcct gccggtcgag
    13021 gaaatggccg aacggggccg tgaaaccctg tctcacggcc cgatgaaacc cgtaggcctc
    13081 aaagacccaa gaacgggaaa gactccctac gcagtggtcc agttgagaaa agaagacaat
    13141 cgggggtcgg cctataacct ggttggcttt cagaccaaac tgaaatggaa ggagcaggag
    13201 cgtgtctttc ggatgattcc cggccttgga aaggccgttt ttctgcgctt cggcagctta
    13261 caccggaata ctttcatcca ctccccgacc cttctggaga agacgcttca gttcaagaag
    13321 gacccgcgga ttttctttgc cgggcagctc accggagtcg aagggtatgt ggagtcaacg
    13381 gccatgggac ttctggcggg aatcaatgcg gcaaggatgg cccgggggaa gactccggcc
    13441 tttcctccac ccacaacctc aatcgggtct ctggtccatt atctgaccga tgcccgggta
    13501 aaggaattcc agcccatgaa tgtcaacttc gggcttttcc ctcccctgga aggtcggatc
    13561 aagggaaggg agaaaaagag ggccatgggc gaaagggctt tgaaagattt gggggaatgg
    13621 attcaagaaa tcggagagcg catatgattt ccaaatcctt tccccacaag gccgatgtag
    13681 tggtcgtggg ggcggggcct gcggggtcga tcctggccta tcaactggcc aaggccggcg
    13741 tcgatgtggt attgctggac aaggccagat tcccccgggg gaagacctgc ggcgggggga
    13801 tcaacgtccg cactcaaaag cagatccctt ttgatttttc ccccatcatc gagggcacca
    13861 tcaccggcat ctctttcacc tgcaaatttg aagacccttt catccggcgc tacaccgcgc
    13921 cgctcatgtt caccgtgcgc cgggagcatt ttgatgaatt cctcgcccgg cgggcggaag
    13981 aggcgggagc tcggtttttc gaccagactc cgttcctgtc ggtggaccaa aagggggggt
    14041 atggaagtgg aaaccgcggt gggggtatgc tcggcggcgt acgttgtcgg ggcggacggt
    14101 gcgcaaagcc tggttgccag gaaaatgccc ctcttttcag acccggccta catcctggcc
    14161 attcacagtg aagcccccgc ctctctgatc cccgactggg accccgccct gattcacatc
    14221 gactggggaa gtgtcaaaag gagctacgcc tacatgtttc ccaagaaata ttttctttcc
    14281 gcgggcgcgg gcggagtcga tgttccggcg ggcaaactca agaactacca tcgggctttc
    14341 ctggccaccc gttcgcagaa ggaccaagcc ctccccttca gcgccgctgg cttcatgatc
    14401 cccctgcgca aaaaccgcag tcccatccac tcgggcaggt gccttctggt cggggatgca
    14461 gccggcctgg cggatccctt ttccggggaa ggaatctttt ccgccgtgcg gagcgcccag
    14521 attgcgtcgt ccgctctgga ggaggccttg aaaggaaatt gtgattccct gaagctttac
    14581 gaggaagcca tcgaccggga tttgatgccc gaattggagt gctcccgcct cttccgggcg
    14641 ttcttcaacc tccgcccttc ccattatcac cggaaaatcg cctcccaaga ccggtggtgg
    14701 aacgccatgg ccaagattat gcggggggag aaaactttcc tggatatcaa gaagaaactg
    14761 ggacccgtgg ggagtctgct gctgaggatg gcgagataag aaaaaggttt gaggaaccga
    14821 ggggttgcca agcgccctat ttttttctgg cctcggacct caaacccagg acctctatcc
    14881 tgtttttcag aaccccatca gtcgaccgac aatctccttg aggatttcgg aggtccctcc
    14941 tccgatggcg ttgatccgta catcccggaa gaagcgttcg atttcatact cccgcatgta
    15001 tccgtaccct ccatggagct ggaggcaagt ggtggccacg aattccccca tccgggaggc
    15061 aaaaaccttg gccatggaca cctcgatggc gcagggttct ttgttcatca acttacgcag
    15121 ggcatgataa ttcagctggc gggcggcttc gatctccatg gccatgtcca ccatcttgtg
    15181 ccggttgacc tgaaacttgc cgatgggccg gccaaaggcc tcgcgctgga gaacataagt
    15241 tttggttcta tcaaagatta gctgggcgct gcacacggtt cccagggcca tgatcagccg
    15301 ctcccactcc aggttggcca tggagttata aaagccgctt ccctcctcgc ccagccgatt
    15361 ccccgctggg acggccacgt tttcaaaagt cagctcggct gtgtccccgg cgtgccatcc
    15421 cagcttctcc aggggtcttc cctgggtaaa tcccgccatt cctttttcta cgatcaaggt
    15481 ggaaaatcct ttgtacccag cctctgggct ggtcttgcag aggaccagca cgaaatcagc
    15541 ccggctgccg ttggtgatga atgtcttgga gccattcaga aggtaatggt ccccctctct
    15601 cttggcgacc gtgcggatat tggccaggtc tgatccggcg ataggctcgg tcaaaccgag
    15661 ggccccgatt ttttctccct tcacccccgg gggcagaaac ctctttttct gatcttcgtt
    15721 tccgaacttg gcaatggccg gaagaacgat gaggccgtgc atcccgatcc cggcttccac
    15781 tcctccggag cggattcggg cagattcttc aacccacacc gaagccatca ggtaatcact
    15841 ccccgaccct ccatactcct cgggaatgag gataccgtgc aatcccatct cccccaccct
    15901 ccggaagacc tcgtcgggaa aatcttgttt tttttcccac tccatcacgt gcggggccag
    15961 ctccttctcc acgaacctcc ggcactgctg gcggaagacc tcgtgctctt ccccgaaggg
    16021 ggtgagcaag aacttttcgc ccatgggggt ttcacctctt tcggttcagt tcatagagca
    16081 agcgcaagcc ttccagggtt aagaactcat ccacctcttc gatggtcttg gattccgagg
    16141 cgatgagcgg agccaacccc ccggtggcga tcaccttggg gttggttttc acttcttctt
    16201 tcatgcggag aacgatttca tcggtcagtc ccacataacc gaagatgatg cccgcctgca
    16261 tcgattgcac cgtattcttc cccaccactc tcttgggtct ctttagctcc acccggggga
    16321 gcttggaggc ccggataaag agcgcctcac tggcaattcc caggcccggg gcaatcgccc
    16381 cgcccatgta ctccccctgg ggagagatgt agtcgaaggt ggtcgctgtc ccgaaatcca
    16441 cgacgatcag gcttctcccg tatttgtggt aggccgccac cgcattcacg attcgatccg
    16501 cgcccacttc ggccggggtg tcggtcagga tcggcatccc caactgaatc ccgacgtcga
    16561 caaccaaagg ctcgacgtga aagtatttct cacagagctc caggagcatg ttcagcatag
    16621 gcgggacgac gcaggagatg gagacagctc cgatgttttc caggggaatc gcccgcgagg
    16681 agaaaagatt gcgcatgagg atcccgtact cgtcgctggt gcggtcccgc tcggtccaga
    16741 tgcgccagct gtccaccagg cgggcgcctt catagatccc caggaccgtg ttggtgttac
    16801 ccacgtcgat taccaaaagc attcggaggc ctccctttca agattcgaat cttccattcg
    16861 caggctgacc tctccggcaa tgaagcgatg ggttttcccc cccccatcga gcaccaaaag
    16921 agccccctcg ggatcaaggc ccaaggcctg gccggata
//
LOCUS       DASPRR010000009        17790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1291735, whole genome shotgun sequence.
ACCESSION   DASPRR010000009 DASPRR010000000
VERSION     DASPRR010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 17790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17790)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17790
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291735"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /gene="katG"
                     /locus_tag="VEL68_00420"
     CDS             <1..1838
                     /gene="katG"
                     /locus_tag="VEL68_00420"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019844723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HXZ34459.1"
                     /translation="NSWPDNVSLDKARRLLWPIKQKYGRKISWADLMILAGNVALESM
                     GFKTFGFGGGREDVWEPDQDVYWGAETTWLGGDKRYSGERDLENPLAAVQMGLIYVNP
                     EGPNGNPDPVAAARDIRETFARMAMNDEETVALIAGGHTFGKTHGAGPASHVGPEPEA
                     AGIEEQGLGWKSSFGKGKGGDTITSGLEVTWTNSPTKWSNNFFRILFGFEWELTKSPA
                     GAHQWKPKGGAGAGTVPDAHDPSKRHGPAMLTTDLSLRFDPAYEKISRRFYENPDQFA
                     DAFARAWFKLTHRDMGPRARYLGPEVPAEELIWQDPIPAVNHKLIDKKDIASLKGRIL
                     ASGLSISQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNQPAELAKVLNTLE
                     DIQRKFNRAASRGKKVSLADLIVLAGCAGVEQAAKKAGHKVTVPFTPGRMDASLEQTD
                     VVSFAVLEPIADGFRNYLKGQYTASAEELLVDKAQLLTLTAPEMTVLVGGMRVLNTNF
                     GQAQHGVFTRKPEALTNDFFVNLLDMSTEWKAVSDAKDMFEGRDRKTGEVKWTGTRVD
                     LIFGSNSQLRALAEVYGSADAQKKFVEDFVAAWTKVMNLDRFDLA"
     gene            complement(2232..3059)
                     /locus_tag="VEL68_00425"
     CDS             complement(2232..3059)
                     /locus_tag="VEL68_00425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXZ34460.1"
                     /translation="MNEFIIGYEPAIRFGFFAGVLALMGFWEWIAPRRPYRTSKKSRW
                     FTNLGIVLIDTLALRLVLPVTAVYVASWNESRSGGFMMQSGLPHWLVILIGVMLLDFV
                     IYLQHAMFHAFPVFWRLHMMHHSDLDFDTTTGVRFHPLEILLSMGIKIGAVFVLAIGP
                     LAVILFEILLNATSLFNHGNVRLPQAIDRVLRLFLVTPEMHRVHHSVIIRETNSNFGF
                     NLPWWDRLFGTYRSAPAAGHEGMTIGLSHFRDPGELTLPRLLILPFTGNPGKYSFGR"
     gene            complement(3197..4966)
                     /locus_tag="VEL68_00430"
     CDS             complement(3197..4966)
                     /locus_tag="VEL68_00430"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HXZ34461.1"
                     /translation="MTTPLEKWVDEVAQMTQPSDIYWCDGSEEEACRLVETGLKREKV
                     EGQPVFYPLNPKTYPNAYLHRSHSTDVARTENLTFICLPEKDMAGPNNNWMDVGKAKN
                     LLKGLSEGCMKGKTMYVLPFTMGHPDSPLAKACVQITDISYVAVSMRIMTRMSNAALG
                     KIGNSENFIRGIHSVGDFDPHRRFIMHFPEDNLVWSVGSGYGGNALLGKKCISLRIAS
                     WQGKQEGWLAEHMIIIGLEDPQGKITYIAAALPSASGKTNLAMLESALPGYKVWTIGD
                     DIAWVRIGADGRLYAINPEFGFFGVAPGTSFKTNPNMMRTLRANSFYPTLYTNMALDA
                     ETKEPWWEGMEDARPEKMLDWRGNLWQPGTPAAHPNSRFTVCIKQCPSLSPEYENPQG
                     VPISAILFGGRRSRVMPLVLESFGWNRGVMMGAIMGAETTAAATGKVGVLRRDPMAML
                     PFCGYNMGDYFAHWLRMGARISSLPKIFFINWFRTDENGKFIWPGFGNNIRALKWIVE
                     RAARSDQRGAKETPIGFVPEKSSMDMTGLSILEQNYQQLFAVNREEWQVELEDARIFL
                     ASFGNRMPKEVWDEFNHLKRAVS"
     gene            complement(5985..7319)
                     /locus_tag="VEL68_00435"
     CDS             complement(5985..7319)
                     /locus_tag="VEL68_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ34462.1"
                     /translation="MEITKALSEYIVKLKFSDLPKDVVEGAKLCFLDWLGVTLSGSKE
                     PLTNILATVAEEQGGKPQATLIGRKKKASLLQAALINGAASHALDFDDVHLGMMGHPS
                     APVFPAILALGEWKKVSGEQFISAFIAGFEAECRVSSIVYPEHYLCGWHATGTLGHFG
                     AAAACANLLGLNLSQTVYALGIAGTQASGIKQVFGTMCKPFHAGKAAMNGLLSALLAE
                     KGFTSSTDMLEGSKGFSKVFSTRMDPPKALERLGDDYAIRGVVFKRHASCFETHPAID
                     AVLALKEEQGLTADQVESIQLDAYTVACDIAGIPAPQTGLQGKFSLAFCVALALGEGE
                     TGEPQFNDEKVRDPRLVALRDRVKIVPDPNLSPSRAKIRVMTKDGRILERSMETLDLS
                     KDRERMKKDLVRKFQDLSTPILGEEKTKKLISRAKRLDKIGKLKALSNLIKG"
     gene            complement(7419..8216)
                     /locus_tag="VEL68_00440"
     CDS             complement(7419..8216)
                     /locus_tag="VEL68_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ34463.1"
                     /translation="MDNNLLLTERKDRAFWITFNRPERRNALSPLLLYELAEVFKKLK
                     QEDEVRCVVLRGAGDKAFSSGYDIAAIPTNVPPEVLEELRKKNPLETAVEAIREFPYP
                     VIAMVNGMAFGAGCEVAITCDIRIAAESARLGMPPAKLGLIYLPAGLMRFVNIVGMAN
                     AKEIFFTGRYFPVARAKEMGLVNYVVPDSQLVSFTEEMAQEVSGNAPLSLKGMKTIFN
                     ALLKYQKIDPQDMKAIELVVAQAFNSEDLKEGQKAFLEKRKPIFKGK"
     gene            complement(8237..9745)
                     /locus_tag="VEL68_00445"
     CDS             complement(8237..9745)
                     /locus_tag="VEL68_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ34464.1"
                     /translation="MGYWDSIILGFSVCLQPLNLLYCFIGVFIGTLIGVLPGVGPVAT
                     ISMLLPATFKMSPVGAIIMLAGIYYGAMYGGSTTSILVNIPGETASVVTCLDGYQMAR
                     QGRAGPALGIAAFGSFIAGTFSVVALTFVAPPLATIAIRFGPPEYFSLLVLGLTLLIY
                     LAHGSMIKALMMACVGLLMSFIGLDNISGYERFTYGIATLEDGLGLVPVAVGVFGLGE
                     VLSNIEESVGKRVIFQTKISNLLPNLQDWKDSAMPIARGTVFGFLLGILPGGGHVLAS
                     FASYAIEKRFSKHPEKFGQGAIEGVAGPESANNAGAGGQFIPLLTLGIPASPVMALML
                     GALVIFGLQPGALLMTRNPDLFWGVIVSMYLGNVMLLVLNLPLIGLWVHVLRVPYPFL
                     FPLIVLFCLIGAYSLSNNIFDVFIMVLFGIIGYLMKKVRFEAAPMLLGMVLGLMMEDA
                     LRQSLIMSGGSFQIFVTRPISAGFIIAALVLLVIPAIRGSKKLLVAKLTGNE"
     gene            complement(9765..10217)
                     /locus_tag="VEL68_00450"
     CDS             complement(9765..10217)
                     /locus_tag="VEL68_00450"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ34465.1"
                     /translation="MQRLDKIGSVVCLIIAGIALWQSMMVPMGTIRQPGPGFLPFWVG
                     VILALLSAVLWLQAEFGKPAAEPVRFLSGEGKWPYVVTAGIALLIYTFLMEPLGFIIS
                     TFLLLIFLLRVIGKQKWWVGVTGSILVTFFTHLIFKVALKVQLPRGLF"
     gene            complement(10233..11195)
                     /locus_tag="VEL68_00455"
     CDS             complement(10233..11195)
                     /locus_tag="VEL68_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34466.1"
                     /translation="MKKGGVVFLLLLIPCLAFGAWPEREITVYNGSTAGGVTDMAARI
                     LSDMVSKMLGQPVVVVNKPGAAHTICANAVANAKPDGYTLGTLSASAFTQVPHYRKVP
                     FDIWKDFTWVGTYTEYTSGLVVKNDAPWKNLNEFLDDAQKNPGKIIYGSDGHGMGTHV
                     LMEYMALKRGGINWKHVPIAGGPKLATALLGGHIHAWSAAGTHVQMIKDKQVRLLVSF
                     NNVRMKAAPDVPTLMELKITDISVGTSLVIIGPKNLPGPILKKLEESYLKAMKDPSYL
                     QYLDRVEFPPVFAGSQETGKSMKHQYDGWGEMIRATGIREEQNK"
     gene            complement(11197..12420)
                     /locus_tag="VEL68_00460"
     CDS             complement(11197..12420)
                     /locus_tag="VEL68_00460"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ34467.1"
                     /translation="MPHLLTNVRILDLSRMLAGPYGSMLLGDLGAEVIKIEEPAGDFT
                     RTPAQYHMGGIGAYFLAINRNKKSVVIDLTTPEGRGVFHDLVKVSDVVFDNLRPAALK
                     KVGADYETLKRISPKIISCSVSGFGQDGPDRDLPAYDLTIQARGGAMGITGEKDGAPA
                     RMGLPMGDLGGGMFAAYAIAAALFDRERTGQGRRLDVSLLDCQASLLTYMATYHSIGG
                     LIPKPQGSAHEVLTPYQAFKAKDLYLVVACPTEKFFQSLCRALGREDLITHPHFKDAF
                     QRIVHRQELCQILQDIFSTKTVDEWMEVLRREDVPSAPVNTLDRVMKDPQLLHRNMVA
                     EVGHPGGGSFKAMGNPVKAEGAEEVLHHPPALGEHTEEILRGLLGYSPQRIQDLIGKK
                     IVAQPAGGEQSKKEG"
     gene            complement(12542..14218)
                     /locus_tag="VEL68_00465"
     CDS             complement(12542..14218)
                     /locus_tag="VEL68_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXZ34468.1"
                     /translation="MFDKKKIEELEKGQQDWKNECLQKSLDKIKMKAEHFPKQFYSPL
                     DIRDLDHARDLGFPGDFPFTMATHPTAIPAEFLVGARARYSGFGTPEDTRDHFKFLVS
                     KGFTGPSVAFDLPTQIGFDSDHPLAAGEVGKIGVACCTLRDMEVIFDAFQRLPMENLR
                     TSFTCNAQTVVILAMYVALAEKKGVALSKLTGTTQNDILKEYVARGTYIFPPGPSMRL
                     VADTLVYGTKYLPRFNVMSISSYHIREAGATAAQELAFMFANAIAYVEAGLKAGLQVD
                     DFAPRLSYLGGCGSHFFGEVAKFRAARRIYARLMKERFGAKDPDSMRLRYPSGNAAHT
                     FTVKKPLNNIVRATVQGMSTLLGGCMGGGYAVAYDEALGLYTEKSRKVADDTPRILRM
                     EARLGDMIDPLAGSYYVEYLTNQVEEEVGKILQKIDSFGGAVKAIEKGYMQQEIARSA
                     YQYQQEVENAKRVIVGVNKFVEEEEPVEGGMAFDPTVEERQLLSLAKVKKERDNLKVR
                     AALDQVRRAAEGTENLMGPVIEAVKVQTTMGEVCGILREVFGEYRPHTQF"
     gene            complement(14254..14772)
                     /locus_tag="VEL68_00470"
     CDS             complement(14254..14772)
                     /locus_tag="VEL68_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012980252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HXZ34469.1"
                     /translation="MKIEIIRGRVWVFGDNVDTDVITPGVYVDAPLEEMKKHVLEALN
                     PRFPKEVKAGDIIVAGKNFGCGSSRETAPDAIKALGVGAVIAGSFARIFFRNAVSIGL
                     PVLPCPGASGSFREGEEAELDVSGAKVTNLSSNRILRGQPLAKDILDILSQGGTLSLL
                     KELAQKEKAKNL"
     gene            complement(14992..16245)
                     /locus_tag="VEL68_00475"
     CDS             complement(14992..16245)
                     /locus_tag="VEL68_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXZ34470.1"
                     /translation="MGMTLAEKILARASGQKEVFPGQYVTAEIDLCMAHEGLAAVFMN
                     LARAGWSKVWDPSRIVSILDHYVPAPTERSAEIHKVVRTAVKQWGIQHFYGERAGICH
                     QVLPEKGHILPGMLIVGTDSHTTTYGAFGAAGTGIGFTDMAYVFATGKLWFKVPETIR
                     FSLKGKLPERVMSKDILLYIAGEYSAEAAQYKAVEFVGPLAEEMSLASRMTMSNMAVE
                     IGAKFGFFVPDRKVSDFLKGRAKNPISLVAPDPDARYEASHEIDASSLEPQVAFPFSV
                     DNVHPVSQAQNIKIHQAFIGSCTNGRLEDLHMAAGILKGKKVHPEVRLLVIPASWEIY
                     RQTMKDGTLDTLIESGAVIGNSSCGPCFGAHMGLLGSDENCIAAINRNFKGRMGSQKA
                     QVFLASPATVAASAIKGYIADPREY"
     gene            complement(16324..17394)
                     /locus_tag="VEL68_00480"
     CDS             complement(16324..17394)
                     /locus_tag="VEL68_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HXZ34471.1"
                     /translation="MTRKKIVALGGDGVGPEVMEVTCHILETAGFDLEIVKPLCGMEA
                     FKAQGNAFPDEAKRLCEESDAILFGAAEGPSVAILVYLRWFMDNYINLRPVKYYPGAL
                     SPLKNPDGINFMLIRENSEGMYPGREGDISWLARKMPDYKDPFDRTLDFYGEGKFALR
                     LITRKGTDRIAKYAANLARERRAQGFAGKVTCITKSNVLRQSCGFFQKIVEEEVKKTP
                     GIGYEHFHVDDAARRLIRFPRDFDVIVTSNMFGDILADEAGEMVGGLGIAPSACLGGR
                     KPYFEPVHGSAPDIAGKGVANPTAMVLSAKMMLDYLGMKDEAQALEKAVAKVYGEGKH
                     LTADQGGTATTKKMAEAVLEKI"
BASE COUNT         3884 a         4984 c         4962 g         3960 t
ORIGIN      
        1 tcaatagctg gcccgacaac gtcagtctgg acaaggctcg ccggctgctc tggccgatca
       61 agcagaagta cggccggaag atttcctggg ccgacctcat gatccttgcg ggcaacgtcg
      121 ccctggagtc gatgggattc aaaacgttcg gtttcggagg cggtcgcgag gacgtctggg
      181 agccggatca ggacgtctat tggggcgccg agacgacctg gctgggtggc gacaagcgct
      241 attctggaga gcgggacctc gaaaatccgc ttgctgccgt gcagatgggt ctaatttacg
      301 tcaacccgga aggcccgaac ggcaaccctg atccggtcgc cgcagcgcgg gatatccgcg
      361 agacctttgc tcgtatggcg atgaacgacg aggagacggt tgcgctcatc gccggcggcc
      421 ataccttcgg caagacccac ggcgccggcc ctgcgtccca tgtggggcct gagccggaag
      481 cggccggcat cgaggagcag ggcctgggct ggaagagcag ctttggcaag ggcaaaggcg
      541 gcgacacgat caccagcggc ctggaagtca cctggaccaa ctcgccaacg aagtggagca
      601 acaacttctt ccggatcttg ttcggcttcg aatgggaact gacgaagagc ccggctggtg
      661 cgcaccagtg gaagccgaag ggtggcgcag gcgccggtac cgtgccggat gcgcacgacc
      721 cgtcaaagcg tcacgggcca gccatgctga ccactgacct ttccttgagg ttcgaccccg
      781 cctacgaaaa gatttcaagg cgcttctacg agaacccaga ccagtttgca gacgcattcg
      841 cccgcgcgtg gttcaagctg acgcaccgcg acatgggccc gcgcgcacgc tatctcggcc
      901 cggaggttcc tgccgaagag ctcatctggc aagaccccat ccccgcggtc aatcacaagt
      961 tgattgataa gaaggacatt gcctccctca aaggcaggat ccttgcttca gggctgtcga
     1021 tctcgcaact ggtgtcgacc gcctgggcgt cggcgtccac cttccgtggc tccgacaagc
     1081 gcggcggtgc gaacggtgcc cgcattcgtc tggcgccaca gaaggattgg gaagtcaacc
     1141 agccggctga actggcgaag gtgctgaaca ctcttgaaga tatccagaga aagttcaaca
     1201 gagccgcttc tcgcggcaag aaggtatcgc tggctgacct gatcgttttg gctggttgtg
     1261 caggcgtcga gcaagctgcg aagaaggctg gtcacaaggt gacggttccc ttcacgccgg
     1321 gacgcatgga cgcctcgctg gaacaaaccg atgtggtctc cttcgccgtg ctcgaaccga
     1381 tcgcggacgg cttccgcaac tacctcaagg gccaatacac cgcatcggcc gaggagttgt
     1441 tggttgataa ggcgcaattg ctgacgttga ccgcaccaga gatgacggtg ctcgttggcg
     1501 gcatgcgcgt gctgaatacc aacttcggac aggcccagca cggtgttttt accaggaaac
     1561 cggaagcgct caccaacgac ttcttcgtga acctgctcga catgagtacg gagtggaagg
     1621 cggtatcgga cgcgaaggac atgtttgaag ggcgcgatcg caagactggc gaagtcaagt
     1681 ggaccggcac gcgtgtcgat ctcatctttg gttcgaactc ccagctccgg gctctggccg
     1741 aggtctacgg aagcgcggac gcgcagaaga agtttgtcga ggacttcgtg gcggcctgga
     1801 ccaaggtgat gaaccttgat cgcttcgacc tcgcctgatc gtagcataaa cctcttcgag
     1861 ggcttttgac agggcggcga aaaaacggtc agctaacaac cggttccagc ggacggctta
     1921 ccgccgccgc tgaaccggaa cgttatccac tctttcaacc agtaattctt gacctccaga
     1981 gcggtcttag gccgaggact cgtaggtgta tccgacgagc ccggccgcta tcctttgatc
     2041 aaattgccaa acgttttcag gttagagatt ttatccagcc tcctcactct ggagataaat
     2101 ttcctagttt tttcctgccc cagaatcggc gtagataaat cttgaaattt acgcaccaaa
     2161 tcctttttca ttctctctcg atctttgggc aggtccaaaa tctccataaa ctttcgagaa
     2221 cctggctgtc tttatcggcc gaaggaatac ttccctggat ttccggtgaa gggcaagatc
     2281 agcaatcgag ggagggtcaa ctccccgggg tcgcgaaaat ggctgaggcc gatggtcatt
     2341 ccttcgtggc cggctgcagg agccgatcga taggtgccaa ataaacggtc ccaccagggt
     2401 aggttaaatc cgaaattgct gttagtttcc cggatgatca ccgaatgatg cactcggtgc
     2461 atttccgggg taaccaagaa aaggcgcagc acccgatcga tcgcttgcgg gaggcgaaca
     2521 ttgccatgat tgaaaaggct ggtggcattg aggagaatct caaagagaat cacagccagc
     2581 ggaccaattg ccaaaacaaa aaccgccccg atcttgatgc ccatcgaaag caggatttcc
     2641 aagggatgga agcgaacccc ggtggtggtg tcaaaatcga ggtccgagtg gtgcatcatg
     2701 tgcaggcgcc aaaagacggg gaaagcatgg aacatggcat gctgcagata gattacaaaa
     2761 tcgagaagca ttacgccgat aaggataacc agccaatggg gcagcccgct ttgcatcatg
     2821 aatcccccac tgcgactctc attccaagaa gccacgtaaa ctgccgtgac tggaagaacc
     2881 aaacgcaagg ccagagtgtc gatgaggacg atccccaaat tggtaaacca gcgactcttt
     2941 ttggatgtgc gatagggccg gcgaggggca atccattccc agaagcccat gagggccaag
     3001 accccggcaa agaaaccaaa ccgtatggcg ggttcgtagc ctataataaa ttcattcatt
     3061 cgattgccct gttcttgaat ttttgaggac cttatcggaa aattttaatc atgaattctg
     3121 tggacgccca aaacagcttc gatggttttc gtgcctgaga gaaaaactaa ccccgccttg
     3181 accggcgtgt taggacctat gaaacggccc tttttagatg attgaactca tcccagacct
     3241 ctttgggcat ccggtttcca aacgaagcca aaaatatccg ggcatcttcg agctcgactt
     3301 gccattcctc gcgatttacc gcaaaaagtt gctggtagtt ttgctccaga atagataggc
     3361 cggtcatatc catgctgctc ttctcgggaa cgaatccgat cggagtttcc ttggcccctc
     3421 tctgatcaga gcgagccgct ctctcgacga tccatttcag ggcccgaatg ttgttcccga
     3481 atccgggcca gatgaatttt ccattttcat ctgtccggaa ccagttgata aagaagatct
     3541 tgggaagaga ggaaatcctt gcccccatgc gcagccagtg ggcaaaataa tctcccatgt
     3601 tgtacccgca gaaagggagc atggccatgg gatcgcgccg caggacacct acctttccgg
     3661 tggccgctgc cgtggtttcc gcgcccataa tggcacccat catcacgccc cgattccatc
     3721 caaagctttc caaaacgagc ggcatgactc tgctccggcg tccgccgaag agaatggcag
     3781 agatgggcac accctgggga ttctcatatt cgggagaaag gctcgggcac tgtttgatac
     3841 agacggtaaa acgggagttc ggatgagcgg ctggggttcc tggttgccag aggttccccc
     3901 tccagtccaa catcttttcc ggccgggcat cctccatccc ttcccaccag ggctccttgg
     3961 tttctgcatc tagggccatg ttcgtgtaaa gggtcggata aaaagagttg gcccgcagag
     4021 tccgcatcat gtttgggttg gttttaaagg acgtccccgg ggctacgccg aagaaaccaa
     4081 actctggatt aatggcataa aggcgcccgt cggcaccgat gcggacccag gcaatgtcgt
     4141 cgccgatggt ccacaccttg tagccgggaa gggccgattc cagcatggcc agattggttt
     4201 ttccggaggc cgaagggagg gcagcagcga tgtaggtgat ctttccttga gggtcttcca
     4261 agccgatgat gatcatgtgt tcggccagcc atccttcctg tttgccttgc caagaagcga
     4321 tgcgcaggga gatacatttt tttcccaaga gggcgtttcc gccgtaccct gagccaacac
     4381 tccagaccag gttgtcctca gggaagtgca tgataaaccg ccggtgggga tcgaaatcac
     4441 ccacggagtg gattccccga atgaaatttt ccgagtttcc gattttgccg agggccgcgt
     4501 tgctcatacg ggtcatgatg cgcatgctga ccgccacata ggagatatcc gtgatttgaa
     4561 cgcaggcttt agccaatggc gaatcgggat gacccatggt aaagggaaga acatacatgg
     4621 tcttcccttt catgcagccc tccgacaatc ccttaaggag gttttttgct tttccaacgt
     4681 ccatccagtt gttgttcggt ccggccatgt ccttctcggg cagacagatg aaggtgagat
     4741 tttcagtgcg agcgacatcc gtcgagtgac tgcgatgcag gtaagcgttg gggtaggttt
     4801 tcgggttcaa cggataaaaa accggctgcc cctcaacttt ttcccttttc aggccagttt
     4861 cgacaaggcg gcaggcctct tcttccgatc catcacacca gtagatgtct gagggttggg
     4921 tcatctgggc cacttcatcg acccattttt ccagaggagt tgtcatatta tggtctccct
     4981 gcatttgtcg tcaaaggaat aaccgcccct gacgggtctg gtctaaaggt taaggaagaa
     5041 gaaatagcga ggtaaaaaaa ggaccttgga aaaataacca attgtcccaa ggaggccagc
     5101 gggaaggtcg tcgagcccga agaccggtcc ccttgtgagc caggcagccg aaagattcaa
     5161 cgctcccact ttagcatatt tgacaccctt tgggaatacg gagagtcccg cgatattctc
     5221 tgcaatccga tttcattatg gtagaagttc cttgcaaagt ggaatttttt ccatagcatc
     5281 tcgtcatgaa ttttccattt tgaagccgga atcattggtt gaaataattt cgaatcattc
     5341 ggatttatta ctaaaaataa aaaagagaac ttttcttcta cctggcactt ttttcgctta
     5401 atagcccttg gtcatgagtt aacgggcagc agggcgagtt aggaaaagac acggtctggg
     5461 gtccgaagat tattcaatga aggaaaggac gcaagactcc ccgctccctg ctccggagca
     5521 gaattttgca gcttttttct gagacgcgac ccaaggattg cttttactat atcaagagct
     5581 cgggggtgca gggtcgcccc ccaaaaggac cgacgatgat gacagaagag aatagattgg
     5641 atcgtcacat acatcttcat tcccatgaga tcatttctgg ccgctggttg aagcgcaggt
     5701 ccaagattcc cgcaaaacta tttccaagat ttgcttgcgg attgtttctc ggcatagcca
     5761 ttgcgttgtg gttccattgg tggaggtgaa gatgcctttg aaagaggaac agggtctggt
     5821 atacagggtt tgccctgaat gtggtcaagg atttttgggg ctctatgcag atagactatg
     5881 ccaaaggtgc agacacaggt tttttgaaag ggacaacatg ctgctgaggg gaaggccggg
     5941 attgggcgac cgggcgaatt ggacttgagg gttacccccg gcctctaccc tttaattaaa
     6001 ttgctcaaag ctttcagttt gccgatcttg tccagccttt tcgctctgga gatcaatttt
     6061 ttcgtttttt cctctcccag aatcggcgta gataaatctt gaaatttgcg gaccaaatcc
     6121 tttttcatcc tctctcgatc tttggacagg tctaacgtct ccatggatct ttcaagaatt
     6181 cggccatctt tggtcatcac tctgatcttg gcccgggagg gggaaaggtt gggatcggga
     6241 acaatcttca ctctatccct cagggccacc aggcggggat cgcggacttt ttcatcattg
     6301 aactgaggct cgccggtttc tccttcaccc agggcgaggg ccacgcagaa agcaaggctg
     6361 aattttccct gcagcccggt ctggggggcc gggatccccg cgatatcgca ggccaccgtg
     6421 taagcgtcga gttgaatgga ttccacctga tccgcagtga gaccttgctc ttcttttaag
     6481 gccagcaccg catcgatcgc cgggtgggtc tcaaagcagg aggcgtgacg tttgaagaca
     6541 accccccgaa tcgcataatc gtcccccaat cgctccaggg ctttgggcgg gtccatccgg
     6601 gtggagaata ctttggagaa tcccttactc ccttccagca tatcggtgga ggaagtaaaa
     6661 cccttttccg ccagcagggc ggacagaagg ccgttcatgg ccgcttttcc ggcatggaaa
     6721 ggcttgcaca tggtgccgaa gacttgcttg atcccggaag cctgggttcc ggcaatgccc
     6781 aaagcataga cggtctgaga gaggttcaaa ccgagcaggt tggcgcaagc agccgctgcc
     6841 ccgaagtgac cgagagtccc ggtagcgtgc catccgcaca ggtagtgttc cgggtatacg
     6901 atggaggaga ctcgacactc cgcctcaaaa ccggcgatga aagcggagat gaattgctct
     6961 ccggagactt tcttccattc ccccagagcc aggatagccg gaaagaccgg ggctgaggga
     7021 tgccccatca ttcctagatg gacatcgtca aagtcgaggg catgagaagc tgccccgttg
     7081 atgagggcag cctgcagcag ggaagccttc ttcttccggc cgatgagcgt agcctgaggt
     7141 tttcccccct gctcctcagc cactgtcgcc agaatgttgg tcaggggctc tttcgacccg
     7201 ctcagggtca cccccagcca gtccagaaaa cagagcttgg ccccttccac tacatccttg
     7261 ggcagatcgg aaaatttcag ctttacgatg tattccgaca atgctttcgt gatctccatt
     7321 ttttctccta tctttcaccg cagagattgc tgagaatacc gagaaagaac cggaaaattg
     7381 aaatgatgga atattggaat ttggtgaact ttctcatttc atttcccctt gaaaataggt
     7441 ttcctttttt ccaggaaggc cttctgccct tctttcaagt cttcgctatt aaaggcctgg
     7501 gccaccacca attcgatggc cttcatgtct tgcggatcga tcttttgata tttgagcagg
     7561 gcgttgaaga tggtcttcat ccccttcaaa gagagggggg cgttcccgga gacctcctgg
     7621 gccatttctt ctgtgaagga aaccagctgg ctgtcgggga ccacataatt caccagaccc
     7681 atctccttcg cccgggcgac cggaaaataa cgaccggtga agaaaatttc tttggcattg
     7741 gccatgccca cgatattgac aaagcgcata agcccggccg gcaggtaaat caacccgagc
     7801 ttggcaggag gcattcccag tctggcggat tcggcagcga tacggatatc acaggtgatc
     7861 gccacttcgc accccgcccc aaaggccatg ccgttgacca tagcgatcac cgggtaagga
     7921 aattcccgga tggcttcaac ggccgtctcc agggggtttt ttttccttag ctcctccaat
     7981 acttccgggg gaacgttggt cggaatggcg gcaatgtcgt aacccgaaga gaaggccttg
     8041 tccccggccc cccgtaggac gacgcagcgg acctcgtcct cttgtttcag cttcttaaac
     8101 acctctgcca gctcgtaaag gaggagcggg gagagtgcat tcctccgctc ggggcgattg
     8161 aaggtgatcc agaaagcccg gtctttcctc tcggtaagca gcaggttatt gtccattctt
     8221 ctctccttct gtgggtttat tcatttcccg tcaattttgc taccaggagc ttcttgcttc
     8281 cgcggatcgc ggggatgacc agcaggacca aggccgcgat gatgaacccg gcggagatgg
     8341 ggcgggttac aaaaatctgg aaacttcccc cggacatgat gagcgactgc cgcagggcgt
     8401 cctccatcat caggcccagg accattccca ggagcatggg agcagcctca aagcgaacct
     8461 tcttcatgag gtacccgatg attccaaaga ggaccatgat gaagacatcg aagatgttgt
     8521 tgctgaggct gtaagcgccg atcaggcaga aaaggacgat gagagggaac aggaaggggt
     8581 aagggacccg cagcacgtgt acccacagcc cgattagggg gaggttcaaa accaggagca
     8641 tgacgttccc gaggtacatg gaaacgatga ccccccagaa caggtcgggg ttgcgggtca
     8701 tgagtagggc gccgggttga agaccgaaaa tgaccagtgc gcctagcatc agggccataa
     8761 ccgggctggc cgggattccc agggtcagga ggggaatgaa ctgcccgccc gccccggcat
     8821 tgttggccga ttccggtccg gccacccctt cgatggctcc ttgcccgaac ttctccgggt
     8881 gtttggaaaa gcgtttttca atggcataag aagcaaagga agcaaggacg tgcccccccc
     8941 cgggaaggat ccccagaaga aagccaaaga ccgtcccccg ggcgatgggc atggccgaat
     9001 ctttccagtc ctggaggttc gggaggaggt tggagatctt ggtctgaaaa atcacccgct
     9061 tacccaccga ctcttcgata ttgctcagga cctcccccag cccgaagacc cccacggcca
     9121 ctgggacaag ccccaggccg tcctctaggg tggcgattcc gtaagtgaag cgctcataac
     9181 cggagatgtt gtccagcccg ataaaggaca tgaggagacc cacacaggcc atcattaggg
     9241 ctttgatcat ggagccgtga gccaaataaa tcagtagggt caggccgagg accaggaggg
     9301 aaaaatattc cggcgggccg aagcggatgg cgatggtggc taggggagga gccacgaagg
     9361 tcagggccac cacggaaaag gtcccggcga tgaaggagcc gaaggccgcg attcccaggg
     9421 ccgggcccgc tcttccctgg cgggccatct ggtagccgtc aaggcaggtg accacggaag
     9481 cggtctcccc cgggatgttc accaggatgg aagtggtgga ccctccgtac atggccccgt
     9541 agtaaatccc ggcgagcatg atgatcgctc ccacgggaga catcttgaag gtggcgggga
     9601 gaagcatgga gatcgtggcc accgggccca ctccgggcag gacgccgatc agcgttccga
     9661 tgaatacccc gatgaaacag tagagaaggt tgagcggctg caggcagacc gaaaagccca
     9721 ggataatgct gtcccagtac cccaagactt cctccccgaa aaaattaaaa taagcctctg
     9781 ggaagctgga cctttaaagc tactttgaaa atgagatggg taaaaaaggt caccaagatg
     9841 gacccggtta ctcccaccca ccatttctgt tttccgatga ctcgcaacaa aaaaatcagg
     9901 agcagaaaag tactgatgat gaaccccagt ggttccatta gaaaggtgta aatcaagagg
     9961 gcgatgcccg cggtaaccac gtagggccat ttcccttcgc cggaaagaaa acggaccggt
    10021 tccgccgccg gtttcccgaa ctccgcctgg agccacagga ccgcggagag cagggccagg
    10081 atcactccca cccagaaggg gagaaacccc ggccccggct ggcggatggt gcccataggg
    10141 accatcatgg actgccaaag ggcgatcccg gcgatgatca ggcagacgac cgatccgatc
    10201 ttgtccagac gctgcaccgc agtctccttt ttctatttat tctgctcttc tctgatgccg
    10261 gtcgcgcgga tcatctcccc ccacccgtca tactggtgct tcatgctttt tcccgtttct
    10321 tgagacccgg caaaaacggg cgggaattcc acccggtcca gatattgcag gtaggacggg
    10381 tctttcatgg ccttcaggta ggattcctcc agctttttca ggatcggccc gggcaaattc
    10441 ttggggccaa tgatcaccag ggaggtaccc acggagatat cggtgatctt caactccatg
    10501 agcgtcggga catcgggcgc tgccttcatg cggacgttgt tgaaactcac cagcagccgc
    10561 acctgcttat ccttgatcat ctggacatgg gttccggccg cggaccaggc gtggatatgt
    10621 ccgccgagca aggccgtggc cagcttgggc cccccggcaa tgggcacgtg cttccagttg
    10681 atcccgcctc tcttcaaagc catgtattcc atgaggacgt gagtccccat tccgtgcccg
    10741 tcgcttccgt aaataatttt gcctgggttt ttctgggcat cgtccaggaa ttcgttcaga
    10801 ttcttccagg gggcgtcatt cttcaccacc agcccggagg tgtactcggt gtacgtgccg
    10861 acccaggtga aatccttcca aatgtcaaaa gggactttgc gataatgggg gacctgggtg
    10921 aaggcgctgg ccgacagggt ccccagggta tacccgtccg gcttggcatt ggccaccgcg
    10981 ttggcacaga tggtgtgcgc cgccccgggc ttattgacta ccaccaccgg ctggccgagc
    11041 atcttgctga ccatgtcaga caggatgcgc gcggccatat ccgtaacccc tcctgctgtg
    11101 gacccgttgt agacggtgat ctctctttcc ggccaggctc cgaaggccaa gcagggaatc
    11161 aaaagaagca aaaagacgac cccccctttt ttcatgttac ccctcctttt ttgattgctc
    11221 tcccccagca ggttgggcga cgatcttttt tccgatcaaa tcctggattc tctgggggct
    11281 gtaccccaga agtcccctga gaatctcttc cgtatgttcc cccagagcgg gaggatgatg
    11341 aagaacttcc tcggctccct cagccttgac tggattgccc attgctttga aacttccccc
    11401 cccggggtgc ccgacctcgg ccaccatgtt ccgatggagg agttgggggt ctttcatgac
    11461 ccggtctaag gtattcaccg gggcgctggg gacatcctcg cgacgcagca cctccatcca
    11521 ttcgtccacc gtcttcgtgg agaaaatatc ctggaggatc tggcagagtt cctgccggtg
    11581 aacgatccgc tggaaggcat ccttgaaatg gggatgggtt atcaagtctt cccggccaag
    11641 agcccggcac agggactgaa agaatttctc cgtgggacag gccaccacta ggtagagatc
    11701 cttcgcttta aaagcctgat agggagtcag gacttcatgg gcggaaccct ggggtttggg
    11761 gatcagaccg ccgatggaat gataagtggc catataggtg aggagcgaag cctggcagtc
    11821 caggagactg acgtccagcc gtcgtccctg cccggtcctt tcccggtcga agagggccgc
    11881 ggcaatggcg taggcggcga acatgccgcc ccccaggtct cccatgggca agcccatccg
    11941 ggctggcgca ccatcctttt ccccggtgat ccccatggct cccccccggg cctggatggt
    12001 gaggtcatag gcgggaaggt cccggtcggg gccgtcctgg ccgaatccgc tcaccgagca
    12061 ggaaatgatc ttcgggctga tccttttcag ggtttcataa tcggcgccga ctttcttcag
    12121 ggcggcggga cgaaggttgt cgaagaccac gtcagagacc ttcactaggt catgaaaaac
    12181 cccccgcccc tcgggcgtgg tcaggtcgat gactacggat tttttgttgc ggttgatggc
    12241 caggaaataa gccccgatcc ctcccatgtg atactgggcc ggagtccggg tgaaatcccc
    12301 ggccggttct tcgattttga tcacctcggc gcccaggtcc ccgagcagca tggacccgta
    12361 agggccggcg agcatgcggg aaaggtcaag gatgcggacg ttggtcagga gatggggcat
    12421 gggatacctc tcatttagtg aaagtgcaag ggaaagggaa agcagaaaaa ggttcttaaa
    12481 ggaagactac cgtaaattct tctttttttc actttcactt acactttcac tttcactgtc
    12541 tttaaaactg cgtatgcggc cggtactccc cgaaaacctc ccgcaggata ccgcagactt
    12601 cccccatagt ggtctggacc ttcaccgctt cgatcaccgg ccccatgaga ttttctgttc
    12661 cctcggcggc cctgcggacc tggtcaaggg ctgcgcgcac tttcagattg tcccgttcct
    12721 tcttgacttt agccagactg agcagctgcc gttcttccac tgtgggatca aaggccattc
    12781 ccccctccac gggctcttct tcctcaacga acttgttgac ccccacgatc acccgctttg
    12841 cattctccac ctcctgttga tactggtagg cgctgcgggc gatctcctgc tgcatgtatc
    12901 ccttttcaat ggctttcacc gccccgccga aggaatcgat cttctgaagg atctttccga
    12961 cttcctcctc cacctggttg gtcagatatt cgacgtaata ggagccggcc agggggtcga
    13021 tcatgtctcc caggcgggct tccattctca ggattcgggg agtatcgtcg gcgaccttcc
    13081 tcgatttttc cgtgtagagc cccagcgcct cgtcataggc cacggcataa ccgcccccca
    13141 tacacccgcc caggagggtg gacatgccct gcaccgtggc tcggacaatg ttgttcaagg
    13201 gttttttgac cgtgaaggta tgggcggcat ttcccgaggg gtagcggagg cgcatggaat
    13261 cggggtcctt ggcaccgaac cgttccttca tgagccgggc atagattctg cgggcggcgc
    13321 ggaatttggc cacctcgccg aagaagtggc tgccgcatcc cccgagatag ctcagacggg
    13381 gggcgaaatc atcaacttga aggccggcct tcaatcccgc ttccacgtag gcgatggcgt
    13441 tggcgaacat gaaggccagt tcctgggcgg cggtcgcgcc ggcctcgcgg atgtggtagc
    13501 tgctgatgga catgacgttg aaccggggca gatatttcgt cccgtaaacc agcgtgtccg
    13561 ccaccagacg catggatggc cctgggggga agatgtaggt cccgcgggcc acatattctt
    13621 tgaggatatc gttctgcgtg gtcccggtga gcttgctcaa ggcaacccct ttcttctccg
    13681 ccaaggccac gtacatggcc aaaataacca cggtctgggc attgcaggta aaggaagtgc
    13741 gcaggttttc catgggcagc cgctggaaag catcgaagat gacctccatg tcccgcaggg
    13801 tgcagcaggc caccccgatc ttacccacct ctccagccgc aaggggatga tccgagtcaa
    13861 agcctatctg ggtgggcaag tcgaacgcga cgctgggtcc ggtgaatccc tttgagacca
    13921 ggaatttgaa atggtcccgc gtgtcctcag gggttccgaa gcccgagtac cgggcccggg
    13981 cgccgaccag gaattcggcg ggtatggccg tgggatgggt ggccatggtg aaggggaaat
    14041 ccccggggaa ccccaggtcc cgggcatggt ccaggtcgcg gatgtccagg ggggagtaaa
    14101 actgcttggg aaaatgctcg gccttcatct tgatcttgtc cagggacttt tgcaggcatt
    14161 cgttcttcca gtcctgttgc cctttttcca gctcttctat ctttttcttg tcgaacattt
    14221 gaagacctcc ctacggcatg gtttaccggc ctcctagagg ttcttagctt tttctttttg
    14281 ggccaattcc ttgagcagag aaagagttcc cccttgggag agtatgtcca ggatatcctt
    14341 ggccagcggt tgccctcgaa gaatcctgtt ggacgacagg tttgtcactt ttgccccgga
    14401 aacatccaac tcggcctcct ccccttcccg gaaggacccg gaggcccccg ggcagggaag
    14461 cacgggaagg cctatgctta cggcgttgcg aaagaagatc cgggcaaagg atccggcgat
    14521 caccgccccg acccccaggg ccttgatggc atcgggggcc gtctcccggc tggagccaca
    14581 gccgaaattc ttgcctgcca cgatgatgtc cccggcctta acttccttgg gaaagcgggg
    14641 attcagagcc tccaggacgt gcttcttcat ctcctccagg ggggcatcca cgtagacccc
    14701 gggggtgatc acgtccgtgt ccacgttgtc cccgaagacc caaacccgac ccctaataat
    14761 ttctattttc atatcctctg gcccctggat gtctgttgca tcgaatgtaa atggatcatt
    14821 caaaaaattc caacttccaa aatccaaata ataccaaaat atcaatgacc aaatatcaaa
    14881 accaggtttt ccgttgagcc ttcttgtgtt ttgggatttg gagcttgttt gggatttggt
    14941 gcttggaatt tggaatttac tttttcccta ttgacttgcc acgctacctc cttagtattc
    15001 ccttggatct gctatatatc ccttgatcgc cgaggcagct accgtcgctg gggacgcgag
    15061 aaacacctgg gccttctggc ttcccatgcg ccccttgaaa ttccggttga tggcggcgat
    15121 gcagttttcg tccgacccca gaagccccat atgagccccg aagcaggggc cgcaggagga
    15181 attcccgatc accgcccccg actctatgag cgtatccagg gtcccatcct tcatggtttg
    15241 ccggtaaatc tcccaggatg cgggaatcac caggaggcgc acttcgggat gcaccttctt
    15301 ccccttgaga atcccggccg ccatgtgcag gtcctccagc cggccgttgg tgcaggagcc
    15361 gatgaaggcc tggtggattt tgatgttctg ggcttgggaa accgggtgga cattgtccac
    15421 ggaaaaaggg aaggcaactt ggggttcaag ggaggaggca tcgatttcat gagacgcctc
    15481 ataccgcgca tcggggtccg gagcgaccag agaaatggga tttttcgccc gtccttttaa
    15541 gaaatcggaa acctttcggt cgggaacgaa aaagccgaac ttagccccga tctccacggc
    15601 catgttgctc atggtcatac ggctggccag actcatttcc tccgcgagag gaccgacaaa
    15661 ctccactgct ttgtactggg cagcttcggc ggaatactct cctgcaatat ataggaggat
    15721 atccttggac atgactcttt cgggaagctt ccccttcagc gaaaaacgaa tcgtctccgg
    15781 caccttgaac cagagtttgc ccgtagcgaa gacgtaggcc atgtcggtga agccgatccc
    15841 ggtccctgcg gctccgaaag ccccgtaggt ggtggtgtga gaatcggttc ccacgataag
    15901 catgccggga aggatgtgcc ccttctccgg caggacctgg tggcagatcc ctgcccgctc
    15961 gccgtagaaa tgctggatcc cccactgttt gaccgccgtg cggacaactt tgtgaatttc
    16021 agctgagcgc tccgtgggcg cgggtacgta gtggtccagg atggaaacga tgcgggaggg
    16081 gtcccagacc ttgctccatc cggcccgggc caggttcatg aagaccgctg ccagcccttc
    16141 atgagccatg cagaggtcga tctccgcagt cacatactgt cccgggaaaa cctctttctg
    16201 cccggaagcc cgggccagga tcttttccgc cagggtcatc cccatggaaa ccttctttta
    16261 agtgtcagtt attagtgtca gtgtcagttc agtctttact gacacgaacc actgaccctg
    16321 acactatatt ttttccagga ccgcctctgc catcttcttc gtcgtggcgg ttcccccctg
    16381 gtccgcggtg agatgctttc cttccccgta gactttggcc accgcttttt ccagggcttg
    16441 agcctcatcc ttcatcccca ggtaatccag catcatcttc gccgaaagga ccatggccgt
    16501 gggattcgcg actcctttgc cggcgatatc gggggccgaa ccgtgcaccg gctcgaaata
    16561 gggcttgcgc cctcctaggc aagcgctggg agcgattccc agccctccga ccatttctcc
    16621 ggcttcgtcc gccaggatgt ccccgaacat gtttgaagtt acgatcacgt cgaaatcgcg
    16681 ggggaaccgg atgaggcggc gggcggcatc gtccacatgg aagtgttcgt acccaattcc
    16741 aggggtcttc ttcacttctt cttccacaat cttctggaaa aaaccgcagg actggcgcag
    16801 tacgttggat ttggtgatgc aagtgacttt cccggcaaat ccctgagccc ttctctcccg
    16861 ggctaggttg gcggcatatt tggctatacg gtcggttccc ttacgggtga tgaggcgaag
    16921 ggcgaacttg ccctccccat agaaatccaa tgtccgatcg aatggatctt tgtagtcggg
    16981 cattttccgg gccagccagg aaatgtctcc ctctctcccc ggatacatgc cttcgctgtt
    17041 ctcccgaatg agcatgaaat tgattccgtc gggattcttc aggggggaga gggcgccggg
    17101 atagtatttc actggccgga ggttgatgta gttgtccatg aaccagcgaa gatagaccag
    17161 gatggcgaca gagggacctt cggccgcgcc gaaaaggatg gcatcggatt cttcgcaaag
    17221 cctcttcgcc tcatcgggaa aggcgtttcc ctgcgccttg aaagcttcca tgccgcagag
    17281 gggtttcaca atttccagat cgaatccggc tgtttccagg atgtggcagg taacctccat
    17341 aacctccggt cccacgccat cccctcccaa ggcaacgatt ttttttctgg tcatgtcttc
    17401 acttttaccg gtttgtctaa atgtttctcg gcacgcgatg atctcgttcc aaatgggagt
    17461 cgaggggaat gagccttttt caacctccga gtcataaatc gaaaagagga atgagaggga
    17521 agtcccataa agggataatc ggagctgatc ggttcggacg atacgttatg atgccagaaa
    17581 caatctgtca aggagaaaga ttctcaccgc ttttctcctg ttcttctaat agccgggatg
    17641 ggcctcagat caaggaaacc gagtgcgggt cgagattttt ttctaaacaa cccctttggc
    17701 tttcagttcc ttcaatctct cttccgaaat cccgagccag tcccgcagga tttcctcgtt
    17761 gtaagcaccc ttgggagggc ccgcaaattc
//
LOCUS       DASPRR010000010         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1936956, whole genome shotgun sequence.
ACCESSION   DASPRR010000010 DASPRR010000000
VERSION     DASPRR010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936956"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1068)
                     /locus_tag="VEL68_00485"
     CDS             complement(370..1068)
                     /locus_tag="VEL68_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34472.1"
                     /translation="MLEVRGIHTYYGLSHILFGISLKVEAGEVVCLLGRNGAGKTTTL
                     KSIIGLTPPKQGSVWFNQEEITGSPPYLLVRRGISYVPDDRRIFADLTVAENLEIARR
                     EPKSGEGWTPDRVYQMFPALRSIESRKGGCLSGGEQKMLAIARALLGNPTILLLDEPT
                     EGLAPVMVRALEGRILSLRASGLTVLLAEQNVRSTLKLSDRGYIIDNGQIRYQGSIQE
                     LRGNEEVRKKYLLI"
     gene            complement(1061..1801)
                     /locus_tag="VEL68_00490"
     CDS             complement(1061..1801)
                     /locus_tag="VEL68_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34473.1"
                     /translation="MLQVERVVKSFDDFLAVNEANLLVQKGEIVAVIGPNGAGKTTLF
                     NLITGQLRRDRGRILFQGEDISELPPYEVCRRGLGRSFQIVNVFHRLTVFDNVQVAVL
                     SHQKKSNTLFHPAGSLAVVETRSLLESVGLADKGRRIVGSLSHGDQKILEIAVALGNN
                     PEMLILDEPTAGMSPEETAATIDLIRHLAKTRGLTILFCEHDMEIVFSISHKIMVMHQ
                     GRTLLQGTPEEVRNDPQVQSAYLGGTDA"
     gene            complement(1802..2791)
                     /locus_tag="VEL68_00495"
     CDS             complement(1802..2791)
                     /locus_tag="VEL68_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34474.1"
                     /translation="MGIITVLINGFKKIGHSNWGWLLIGMFILLFLMPPFVPRFFVYI
                     LGLIYVTSLLAMSLNFLVGFGGMFQFHHAVFYGVGAYTVALILTRTSLPLWVGFVAGP
                     VAAGLVGLLIGWFCVRLTQLYFGMLQISLGSLIWAIVYRWYKFTGGDDGIHGIPMPQA
                     LSSLNQAYYFILILLVVSLVLLYSILKSPFGSTLQAIRDNPQRCAAVGVNVRRHQLRA
                     IGIGTFFAGIAGVLFVVLEKSVFPDLLFWTLSLEVFIMCLLGGWFTFGGPILGAAITV
                     ALRTFVGKYTEYWTLILGVILILLIFFLPEGVMGYFVEKFKSRENQGSERGIS"
     gene            complement(2815..3708)
                     /locus_tag="VEL68_00500"
     CDS             complement(2815..3708)
                     /locus_tag="VEL68_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34475.1"
                     /translation="MGFLPNIGPAEVLSQLLVGLSRAMILFTVCSGLSFVLGVLRVPN
                     IAHGSLYMIGAFATFSLSKWIGGPAGFWLALLLAPLVVALISLIAERALFRHLYQREH
                     LMLLLFTFALALILGDLTKIVWGAEYRSVSAPDLLKGSVSLLGASFPRYNLFLIFVGL
                     AVAVALWLFVNRTKIGKVSRAAAVDRDMVGLMGINVSWVFAIAFVIGCCLAGLGGALV
                     APTVSITLGMDHTLIIEAFLIVTIGGLGNMWGALVGSLIFGVTQSLGVLVFPQFAIVF
                     PYAAVVIVLLLRPKGLLKSVW"
BASE COUNT          915 a         1091 c         1130 g          742 t
ORIGIN      
        1 tgagcctaac gtatagcaaa aaggttatta gcgctagtgt cgcgtctcag aaataagctg
       61 cataattttg cccccgagca gggagcgggg cgaagaaccg ctgcgcttgc caaaataaga
      121 agctcgttcc tctttggaaa agttgattct tactaaaggt ttcggaagtt gcctattttt
      181 gcgctcttag cctaattccc ggaggctggc agcgtcgctt tgcggatacc aggccaccgc
      241 tcggagacgg gccatttccc gggcgatttt ggcggcgcgc atcgtttctc cgcccccctc
      301 ctggtaaatt tattcactga acttagggga cgcgacacca ggccccattt gctattctct
      361 ctgccttttt tagatgagca ggtatttttt cctaacttcc tcattccccc gcagctcttg
      421 gatgcttccc tggtagcgga tctgaccgtt gtcgatgatg tacccccggt cgctcaattt
      481 gagggtcgaa cggacgttct gctccgcgag caagaccgtc agcccggagg ctcttaggct
      541 caggatccgg ccttccagcg cgcggaccat gaccggcgcc aggccttcgg tgggttcatc
      601 gagaaggagg atggtgggat tgcccaggag cgcccgggcg atggccagca tcttctgctc
      661 gcccccgctc aggcatcccc ctttccggct ttcgatgctg cgcagcgcag gaaacatctg
      721 gtacacccgg tccggcgtcc acccttcgcc gctcttaggc tcccgcctgg cgatctccag
      781 gttttctgcc acggtgaggt cggcgaagat ccgacggtca tccggaacgt agctgattcc
      841 cctgcgaacc aagagatagg gggggctgcc ggtgatctcc tcctgattga accagacgct
      901 tccctgtttg ggcggagtca acccgatgat gctcttgagg gtggtggtct tacccgctcc
      961 attccttccc agaagacaaa ccacctcgcc ggcctcgacc ttgagggaga tcccgaaaag
     1021 gatgtgactc aacccgtaat aggtgtggat tcccctgact tcaagcatcg gttcccccga
     1081 ggtaagcgga ctggacctgc gggtcgttgc gcacttcttc cggagtcccc tgaaggaggg
     1141 tccgtccctg atgcatgacc atgattttgt gcgaaatgga aaagacgatc tccatgtcgt
     1201 gttcacaaaa aagaatggtc aatccccggg tcttggccag gtgacggatc aggtcgatgg
     1261 tggcggcggt ctcttccggg gacatgccgg ccgtgggctc gtcaaggatc aacatctcgg
     1321 ggttgtttcc gagggcaacg gctatttcca agattttttg gtctccgtgg gaaagagaac
     1381 ctacgatgcg gcggcccttg tccgccagac ccacgctttc cagaaggctc ctcgtttcca
     1441 cgaccgccag gctgccggcc ggatgaaaaa gggtgttgct cttcttctga tgggacaaaa
     1501 cggctacctg cacattgtcg aagaccgtga gacggtgaaa cacgttcacg atttgaaagg
     1561 accgccccag ccccctgcgg cagacctcat aggggggaag ctcactgatg tcttctccct
     1621 ggaaaaggat tcggccccgg tcccgccgga gctgaccggt aatgaggttg aagagggtgg
     1681 tcttccccgc gccgttaggg ccgatgactg ccacgatctc ccctttctga accaggagat
     1741 tggcttcgtt caccgccaga aagtcatcga aagatttaac caccctttcc acctggagca
     1801 tctaagagat acccctttcg cttccctgat tttcccggga tttgaatttt tccacgaaat
     1861 accccatgac tccctcgggc aggaagaaaa tcaggaggat gaggatgact cccaggatca
     1921 aggtccagta ctcggtgtat ttccccacaa aggtccgcag ggccacggtg atggctgccc
     1981 ccaaaatggg tccgccgaag gtgaaccagc cccccaaaag gcacatgatg aagacctcca
     2041 gggagagggt ccagaagagg aggtcgggaa agacggattt ctccaggacg acaaagagta
     2101 cgccggcgat accggcgaag aaagtgccga ttccgatggc tcggagctga tggcggcgaa
     2161 cgttcacccc cacggctgcg cagcgctggg ggttgtcgcg gatggcctgc agggtacttc
     2221 cgaaggggga tttcaggatc gaatagagca acaccaggga gacgaccagg agaatcaata
     2281 tgaagtagta ggcctggttt agggaggaaa gagcctgcgg catggggatg ccgtgaatgc
     2341 cgtcatcgcc cccggtaaat ttgtaccagc ggtagacaat ggcccagatg agcgagccca
     2401 gggagatttg gagcatgccg aagtacagct gggtgagccg gacgcagaac caaccgatga
     2461 ggaggcctac cagtccagcg gcgacaggcc cggccacaaa tcccacccaa agaggcaggg
     2521 aagttctggt caggatcagg gcgaccgtgt aggcccccac gccgtagaag acggcatggt
     2581 gaaactggaa catgccgcca aaccccacca ggaagttcaa actcatggcc aggagagagg
     2641 tgacatagat caaacccaga atgtaaacga aaaagcgggg gacaaagggc ggcatcagaa
     2701 agagaaggat gaacatcccg atgaggagcc aaccccaatt tgaatggcct atttttttga
     2761 acccgtttat caggacggtg atgattccca tttacgctat gccctctgct ctttctacca
     2821 gacggatttc agcaggcctt tggggcgcaa gagtaatacg atcaccaccg cggcgtaagg
     2881 gaaaacaatg gcgaactggg gaaacaccag gactccgaga gactgggtca cgccgaagat
     2941 tagcgatccc accagcgccc cccacatgtt gcccagtccg ccgatggtca cgatgaggaa
     3001 ggcttcaatg atgagggtgt ggtccatgcc cagggtgatg ctcaccgtgg gggccaccag
     3061 cgcccctccc agccctgcca gacagcaccc gatcacgaaa gcgatggcga aaacccagct
     3121 cacattaatt cccataagcc ccaccatatc ccggtccacc gccgccgccc gggagacctt
     3181 gccgatcttc gtccggttca cgaaaagcca gagagcgacg gccacggcga gtccgacgaa
     3241 gatcaagaag aggttgtagc gggggaagga ggcccccagg agggagaccg agcccttgag
     3301 caggtccggg gcggaaaccg accggtattc cgccccccac acaattttgg tcaggtcccc
     3361 caggatgaga gccagggcga aggtaaagag gaggagcatc aggtgctcgc gctgatacag
     3421 gtggcggaag agggctctct ccgcgatcag gctgatgagg gcaaccacca gaggggccag
     3481 gagaagggcc agccagaaac cagcgggccc tccgatccac ttcgagaggc tgaaggtggc
     3541 gaaggccccg atcatgtaca gggaaccgtg agcaatgttg gggacccgga ggacgccgag
     3601 gacgaagctc agtcccgagc agacggtgaa caaaatcatg gccctgctaa ggccgaccag
     3661 cagttgcgaa aggacctcag ccgggccgat attgggtaaa aaacccacct ctgacctcac
     3721 ctaacccttt attgccgttc atggaaatgt gggaagaatg tacggaggtc aatgaaaatt
     3781 cgaaatccga agcacgaaat ccgaaacaaa tccaaaagtc taaaaaagca atgctctaaa
     3841 cgaagaaagt tttgtattta gaattttggg tattgaac
//
LOCUS       DASPRR010000011         5378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_484931, whole genome shotgun sequence.
ACCESSION   DASPRR010000011 DASPRR010000000
VERSION     DASPRR010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484931"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VEL68_00505"
     CDS             complement(<1..720)
                     /locus_tag="VEL68_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34476.1"
                     /translation="MMKKAGFILVSIFSVLFTFCVSNNWAQPYPAKPVNMIIGWGAGG
                     ATDITLRTLCEAAGKTLGQPIVVMNKPGGGSAVALALLKNEKPDGYTVGNLSAAGILS
                     QYMRKVPYDVMTDFTPIMRYGDYTYGVVVRAEAPWKAFKELVDYAKANPGKVRYSTSG
                     AGTFHHLAMEALAKQEGIKWVHIPYKGGHEATTAILGGHVEVEACSSEWKPHVESGKL
                     RLLATYNPTRLPKFPDAPSWVE"
     gene            complement(857..2752)
                     /locus_tag="VEL68_00510"
     CDS             complement(857..2752)
                     /locus_tag="VEL68_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXZ34477.1"
                     /translation="MKAAKKAKKAMLIGLDGMILPFVERFVREGKMPTFKKLMKEGSF
                     GSAWSEYPTATQPNWTVIQTGAYPGKSGVYDMTIHVPGEPLDVVHTGFDSRFHKAEYL
                     WQVVDRLGKKAFLLKYPGTWPPRVKHGYQIDGHCAPAGIVDHHWGSSAVAIHPSRVYS
                     TDNYTRSVKVELKPQSGWANPPASHSPALEDTIPIAPGAGDTPFSILAIDSAGEGYDR
                     LVISPAKDGKKAVANLGQNEWSGMIHASFRQGKKKRSGAFRMKLMDLSPDGRKVKLYC
                     SQVYPTEGFAHPKSLEKKLVKEVGPFIEYIGLQPYIFGWTNEETWVEEMEYHTSLMEK
                     YASYVFKNHPWDFFAMQWHAIDFAKHQFWHFDPAGSYYDPDTADMKWKIIETTYIAAD
                     RLVATMLKHATPDTLVAVVSDHGHLPGVQEFLINNILIKAEMVTLKTRVNERGEEETL
                     REPDFSKTKAFAQYGWAIQVYVNLKGRDPEGIVEPGEEYEKVRDEIIRLLYSAYDSKL
                     GKHPVILALRREEAEPLLHCGPNAGDVIYILDPHYEASATIRIPPTEYEKPHFASIHS
                     TVLPNAVLGMGTIEAMYIFRGPGVRKGWRNPDPVRLIDIAPTVSYGLNIPAPAQADGR
                     AVYELFK"
     gene            complement(3426..4613)
                     /locus_tag="VEL68_00515"
     CDS             complement(3426..4613)
                     /locus_tag="VEL68_00515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ34478.1"
                     /translation="MREVVIVDGVRTAIGRMGGSLANFRAEDLAALAIEGVVKKTKID
                     PLEIEDVIMGIALSWHSAVNLCRWAALKAGLPFSVPGLTVERQCGSGLQAVALAAQRI
                     LAGDGEVFIAGGTESHSTFPYKVARPTQAFQYNPPGFINWETAPRPEWNLVMGITAEN
                     LAEQWKVSREWQDEFSYYSQMRAIKAIQSGRFKEEIMPVMVPQRKGDPVAFEVDEHPR
                     ADTAIDKLSKLAPVFKKGGTVTAGNSSGLNDGAAALLIMSMEKARSLGYKPLARILSS
                     AVVGVDPRIMGIAPAYAIPKALQRASIKFDQVDVVECNEAFAAQTLAVVKEMENQGMK
                     VNLEKLNPNGGAIALGHPNGMSGARLTLTLIRELQRRNGRYGVASLCIGGGQGIAAVF
                     ERV"
     gene            complement(4663..>5378)
                     /locus_tag="VEL68_00520"
     CDS             complement(4663..>5378)
                     /locus_tag="VEL68_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ34479.1"
                     /translation="FFILNGSKTFITNGPIGDLFLVYATTDPKSRGKGVTAFVVEKGF
                     PGFSCGKEFEKLGWHGSPTGELIFEDCPVPRENLIGTVNQGVKVLLSGLNSERLVISA
                     NCLGLARASLEDSVKYSRERKQFGKAISEFQMIGEKLARMAAKVEAVQCMLWSQALRM
                     SQEGPAAMAFETACTKLLASETSLQNALDATQIFGGYGYIREFPVERYLRDARIMTIG
                     AGTSEIMCQIIFRELSKKI"
BASE COUNT         1097 a         1535 c         1515 g         1231 t
ORIGIN      
        1 ttcaacccag gagggggcat cgggaaactt gggtaagcgg gtggggttat aggtggccag
       61 gagcctcaat ttgccgcttt ccacatgggg cttccattcg gagctgcagg cttcgacctc
      121 cacatgccct ccgaggatgg ccgtggtcgc ctcgtggccc cctttgtagg gaatgtggac
      181 ccatttgatt ccttcctgtt tggccagggc ctccatggcc aggtgatgga aagttccggc
      241 tccggaagtg ctgtatcgga ccttgccggg gttggccttg gcgtaatcga ccaactcttt
      301 gaaggctttc cagggggctt ccgctcgaac gaccaccccg taggtgtaat ccccgtaccg
      361 catgatgggg gtaaaatcgg tcatcacgtc ataggggacc ttccgcatgt actggctcag
      421 aattcccgcg gcggaaaggt ttccgaccgt gtatccgtcg ggcttctcat tcttcaaaag
      481 ggctagagcc acggctgagc ctcctccggg tttgttcatc accacaatcg gctggcccag
      541 ggtcttgccg gccgcttcac aaagggtgcg cagggtaatg tccgtggctc ccccggcgcc
      601 ccacccgata atcatgttga ccggtttcgc gggatagggc tgcgcccagt tgttggagac
      661 acagaaggtg aaaagaaccg aaaaaataga taccaagatg aaccctgctt ttttcatcat
      721 tcaacctcgc tgtgcaaaaa tccgctccgg ccatcggctc cgagcaaggg aacctgactt
      781 tcggccatat gatcccgaga agcgggacca gattgaatca gcgatgcatg aactaccctc
      841 ggttcagcca ctctcctcat ttgaataatt catataccgc tcttccatcc gcctgggccg
      901 gggccgggat gttcaagccg tagctcaccg tcggggcgat gtcgatcagc cggacggggt
      961 cggggttgcg ccatcccttg cggacccccg gaccgcggaa aatgtacatg gcctcgatgg
     1021 ttcccattcc cagcacggcg ttgggaagga cggtggagtg gatggaggcg aagtggggtt
     1081 tttcatattc cgtagggggg atccggatcg tggccgacgc ttcgtagtgg ggatcgagga
     1141 tatagatgac gtccccggca ttcggcccgc agtgcagcag gggctcggct tcctcccgcc
     1201 taagagccag gatcacggga tgcttgccca gcttcgaatc ataggcgctg tagagcaggc
     1261 gaatgatttc gtcccggacc ttttcatatt cttccccggg ttccacgatt ccttccgggt
     1321 ctctcccttt caggttcacg tacacctgga tggcccagcc gtactgggcg aaggctttgg
     1381 tcttggagaa gtccggttcc cggagagtct cctcttcccc cctctcgttc acccgggttt
     1441 tgagggttac catttctgcc ttgatcagaa tattgttgat caggaactcc tgaaccccgg
     1501 gcagatgccc atggtcggag acgaccgcca ccagggtgtc aggagtggca tgtttcagca
     1561 tggtggctac caggcggtca gcggcgatgt aggttgtctc aataatcttc cacttcatgt
     1621 ccgccgtatc cgggtcgtag taggacccag ccgggtcaaa gtgccagaac tggtgcttgg
     1681 caaagtcaat ggcgtgccat tgcatggcga aaaaatccca gggatgattt ttaaagacgt
     1741 aggaggcgta tttttccatc agggaggtat gatattccat ctcctccacc caggtctcct
     1801 cgttggtcca gccgaagatg tagggctgaa ggccgatgta ctcgatgaag gggcccacct
     1861 ccttcaccag ctttttctcc agggatttcg gatgggcaaa gccctccgta gggtaaacct
     1921 gggagcagta gagtttgacc tttcttccgt ccggggagag gtccatcagc ttcatccgga
     1981 aggccccgct ccgtttcttt ttgccctgtc tgaaggaggc gtggatcatc cccgaccact
     2041 cattttggcc caggttggct actgccttct tcccatcctt tgcgggagag atcaccaagc
     2101 gatcgtatcc ctctcctgct gagtcgatgg ccagtatgga gaagggcgta tccccggcgc
     2161 ccggggcgat gggaatggtg tcttccaggg cgggggaatg gctggccggg gggttcgccc
     2221 acccgctttg gggtttcagt tccactttca ccgagcgggt gtagttgtcg gtggagtaga
     2281 cccgggaagg atggatggcc acggcactgc ttccccagtg atggtccacg atgcccgccg
     2341 gggcgcagtg tccgtcgatc tggtagccgt gcttcacccg gggaggccag gtcccaggat
     2401 atttgagaag aaaagccttt tttcccaggc ggtccaccac ctgccagagg tattccgcct
     2461 tgtggaagcg cgagtcgaaa cccgtgtgaa ccacgtcgag gggctcaccc gggacatgga
     2521 tggtcatgtc atagacaccg ctcttgccgg gataggcgcc ggtctggatg accgtccagt
     2581 tgggctgggt ggcggtgggg tactccgacc aggcggaccc gaagctcccc tccttcatga
     2641 gctttttgaa ggtcggcatc ttgccttccc tgacgaaccg ctccacaaag ggaagaatca
     2701 ttccgtccag tccgatgagc atggcctttt tggccttctt ggcagctttc atatagccct
     2761 cccttgtgat ttcccccctt cttactggat cagaaagaga aaggggcagt caaaatggct
     2821 ggtccttccg gtaagcgatt tatgggttct attatttatc gcggaatcca taaaaatgca
     2881 actcccattt gaaaggaccg aattcatttc gtgagagatt tcaaattgat aatcttttct
     2941 tagtatgccc ccaatctgaa gaatttttcc cgaagtaccg ggaccctgaa tcaaattggc
     3001 gaccccccag agacttagag gaggaggggg gaatgaaaac aataaaagca gggtcaaaaa
     3061 agcgcttttc gggtgatttt tcaaagcgga ggaaatggct ggaagggcgc ctctctttga
     3121 tccctccgga gggtgggtgg tcaaagaccc tcccgggaaa gggaagggaa attgggccgg
     3181 ggctcccagc tccttttaag atgaggaaga aggccgattc ttcctgccct accgccttcg
     3241 gaaaccatta actgagggaa gggggtattt gacctgcgtc gcggagagca aagatgcgag
     3301 ggaattcaac gaaacctgga ccggaaaagc ggccgagttc gattctccct ccatcgagag
     3361 atcggcactc atcaaggctc cggagggggg gcaccgttca caacctagat ccttcatgtt
     3421 tttacctaca ccctctcgaa caccgccgcg atcccttgtc ctccaccgat acacaaggac
     3481 gccactccat accttccatt ccgcctttgg agttcccgga tcaaagtcaa tgtgaggcgg
     3541 gctccgctca tcccgttcgg gtgaccgagg gcaatggcgc ccccgttggg gttgagcttt
     3601 tccaaattga ctttcatgcc ctggttttcc atttctttta cgaccgcgag agtctgggcc
     3661 gcaaaggctt cattgcactc tactacatcc acctggtcga attttatact ggcccgctgc
     3721 agagccttgg gaatcgcata cgccggggca atgcccatga tccgcgggtc tactccgaca
     3781 accgcggagg aaagaatcct ggccagaggc ttatagccaa ggctcctggc tttttccatc
     3841 gacatgatga gcaaagctgc cgccccgtcg ttcaagcccg aagaatttcc tgcggtgacg
     3901 gtcccccctt ttttaaaaac cggggcaagt ttggacagtt tatcgattgc ggtgtccgct
     3961 ctgggatgtt catctacctc gaaggcgacg gggtcccctt tgcgctgggg aaccataacc
     4021 ggcataatct cttctttgaa gcgacccgat tggatagcct tgatggccct catctggctg
     4081 taataggaga actcgtcctg ccattcccgg ctcaccttcc attgctccgc cagattttcg
     4141 gcggtaattc ccatcaccag gttccactcc ggacgggggg ccgtttccca gttgataaac
     4201 cctggaggat tgtactgaaa ggcctgggtc ggccgggcaa ctttgtaggg gaaggtagag
     4261 tggctctcgg tcccgcccgc gatgaagacc tccccatcgc cggccagaat ccgttgagcg
     4321 gccaaggcca cagcctgcag ccccgagccg cactggcgtt caaccgttag cccgggaacg
     4381 gaaaagggca ggcccgcttt caaggcggcc cagcggcaga ggttaacggc tgagtgccag
     4441 gataacgcaa tccccatgat gacgtcttcg atctccagcg ggtctatttt cgtcttcttg
     4501 accacgcctt cgatggctaa ggcagccagg tcttcggccc ggaaattggc caggctgccg
     4561 cccatccggc cgatggccgt cctcacccca tccacaatca caacttcacg catattgacc
     4621 tcccgattga accttcagga aaaatcaccg ggtaattgac atctaaattt ttttgctcag
     4681 ttccctaaaa atgatctggc acatgatttc cgaagttccc gccccgatgg tcatgattcg
     4741 agcgtcccgg agataccgct ccacggggaa ctctcgaata tatccgtatc cgccgaatat
     4801 ctgagtggcg tccagcgcgt tctggaggga ggtctccgac gccaggagct tggtgcaggc
     4861 ggtctcgaag gccatggccg caggcccttc ctgggacatc cgtaaagcct gggaccagag
     4921 catgcactgc accgcttcca ctttggcggc catccgggcc agtttttcgc cgatcatctg
     4981 aaactcggaa atggcttttc caaattgctt tctttcccgg gaatatttaa ccgaatcttc
     5041 caggctggcc cgggccagac ccaggcaatt ggccgagatc accagcctct cggagttcaa
     5101 ccccgagagg agaaccttga ccccctggtt gacggtcccg atgagattct cgcggggaac
     5161 cggacagtct tcgaagatta attctccggt gggtgagcca tgccagccga gtttctcaaa
     5221 ctccttgccg caggagaaac ccggaaaccc tttctccacc acaaaagcgg taaccccctt
     5281 gcctcttgat ttggggtcgg tggttgcata tacgagaaaa aggtctccga tcggtccatt
     5341 ggtgatgaag gtcttgctcc cgttgagaat aaagaaat
//
LOCUS       DASPRR010000012         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1293419, whole genome shotgun sequence.
ACCESSION   DASPRR010000012 DASPRR010000000
VERSION     DASPRR010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293419"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..557)
                     /locus_tag="VEL68_00525"
     CDS             complement(36..557)
                     /locus_tag="VEL68_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXZ34480.1"
                     /translation="MIRGRVHKFGDHIDTDGIIPAGCSHISDPAELGRHCMENIDPAF
                     ARRVSPGDIIVAGSNFGCGSSREFAPWAIHGAGISCVIAKSFARIFFRNAINIALPLL
                     TCPEAVEGIEEKDLLEIDLGRGEIRNLTRTTVFTALPYPEFIRELISAGGLLEYGRRL
                     LAQSRESKEPIRK"
     gene            complement(554..1813)
                     /gene="leuC"
                     /locus_tag="VEL68_00530"
     CDS             complement(554..1813)
                     /gene="leuC"
                     /locus_tag="VEL68_00530"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXZ34481.1"
                     /translation="MTLAEKILAAHTGRKSVKPGEFLNVPVDLVMTTDGTGLIAVRQF
                     QKMGARRVFNPSKVVLVLDHSVPAPNVDAAETSRVLREFAFQYGTLLYDVGRSGIVHV
                     LLPEQGLVLPGDVVAGGDSHTCTYGALGAFATGMGATDIAFAMAFGQTWMRVPESIRL
                     VYHGHLQPWVGGKDLILHTISRIGVEGANYKAIEFAGPVIEALKMDERFTMANMAVEA
                     GAKAGLFLVDEETLAYVKKRAKRPYTVYHPDPEAEYAETVEIDISSLEPQVAFPHSPA
                     NARPVTEAKGIEIVQAVIGSCTNGRLSDLRMAARVLAGRRIHLRVRCIVLPGSQEVYL
                     EALREGLIETFIQSGAAVSTPTCGPCVGAHMGVLAAGEKCISTTNRNFIGRMGSPKSE
                     VYLANPAVVAASAVQGRIVHPQEVLGT"
     gene            complement(1810..1968)
                     /locus_tag="VEL68_00535"
     CDS             complement(1810..1968)
                     /locus_tag="VEL68_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34482.1"
                     /translation="MKKTSLLEKHILAPEILVMPRAHDALSARIIENAGFKALFIGGY
                     PGRKRSVR"
     gene            complement(2225..3712)
                     /locus_tag="VEL68_00540"
     CDS             complement(2225..3712)
                     /locus_tag="VEL68_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34483.1"
                     /translation="MEIPAESKVHIIIRCPQCGGTIDFLEEAQVIHCQFCGTELLVAG
                     REGVLRYVLSPQISDPRKAQSLAGEHLLKLGRRSPRIGETLLFYAPFWRLQGTVYRWV
                     FGLKPLKMESEAGVPPPMERFKILLVRVMDHTIPAYWDLEIGLSSLGVRPQVLRLQPF
                     SPEHLEMRDSFLPLDLPLEAVQAQVGRYVDFFFQAEKMISEVNLERIVGLQFSVIYFP
                     LWFVECLHEEGCEILLLDAVGQSPIQMIPDGSPILAKLRGNETRKPFEFSELRFLPFR
                     CPNCGWAFPFFPLSVLHFCPTCRHLFREKGGDWAEMNYSAVPPAGERLPKDLLWVPFW
                     RCRTTLESSGERLASMADLYRLAPPPRVFNPERESRRPIFFYFPAVKFRNPQTIHNLG
                     SRLTFLQPDVPLGCFEDGSHPLTAGGSLGIKDAQELGMMILGALIPQANRKARAWIKD
                     CRVELQDSQLVFFPFTRADLFWKEMTSGISFQGNALSEDLPEIAR"
     gene            complement(3795..>4218)
                     /locus_tag="VEL68_00545"
     CDS             complement(3795..>4218)
                     /locus_tag="VEL68_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXZ34484.1"
                     /translation="DFLKYKAAKALGDAAKAEGGGAAGAAASSGMGLGVGAGLGMMLP
                     GMLMKTMQEGSMPSEKKIPCPKCHSEISIDARFCPYCGTQIVKVNKCLHCGKDLPLEA
                     NFCMVCGAKVEKPNITCSKCGTQALPGAVFCNQCGEKL"
BASE COUNT          939 a         1218 c         1202 g          859 t
ORIGIN      
        1 gaatgccaga agaagcaggg tcagcagaag gggaatcatt ttcgaatcgg ttcctttgat
       61 tcgcgcgatt gcgccaaaag gcgtcggcca tattccaaca gccctcccgc ggaaatcagc
      121 tcccggatga attccgggta gggaagggcg gtaaagaccg tggttcgggt caaatttctg
      181 atttcaccgc gacccaggtc gatttccagg aggtccttct cctctattcc ttcgactgcc
      241 tccggacatg tcagaagcgg cagggcgatg ttgatggcgt tgcggaaaaa aatgcgggca
      301 aagcttttgg cgatcacgca ggagatgccg gccccatgga tggcccacgg ggcgaactcc
      361 cgggaagagc cgcatccgaa attgcttccg gccacgatga tgtcccctgg cgagacgcgc
      421 cgggcaaagg ccggatcgat attttccata caatgacggc ccagctcggc cgggtcgctt
      481 atgtgagaac acccggcggg gatgatcccg tccgtgtcga tgtggtctcc gaatttgtgg
      541 acccgtcccc ggatcatgtt cccaaaacct cttgcggatg gacgatccgg ccctgtaccg
      601 cgctggcggc gactacggcc gggttggcca ggtagacttc agacttcgga cttcccatcc
      661 gcccaataaa atttcgattt gtcgtggaaa tgcatttttc accggcggcc aagaccccca
      721 tgtgagctcc cacgcagggg ccgcaggtgg gggtgctgac cgcagctccc gactgaatga
      781 aagtttcgat caatccttcg cgcaaagcct ccagatagac ctcctgggaa ccggggagaa
      841 caatgcaccg cacccgaaga tggattcgtc tgcccgccag gacccgggcc gccatccgta
      901 agtcactcag gcggccgttg gtgcagctgc cgatcaccgc ttgcacaatt tctattccct
      961 tcgcttcggt taccggcctt gcattggccg gagaatgcgg gaaagccacc tgcggttcca
     1021 gggaggagat atcgatctcc accgtctcgg cgtattctgc ctccgggtca ggatggtaaa
     1081 cggtataagg ccgcttggcc cgcttcttca cataggccag ggtctcctca tccacaagaa
     1141 aaagccccgc cttggcccct gcttcgaccg ccatgttcgc catggtgaaa cgttcgtcca
     1201 ttttcaaggc ttcgatcacg ggaccggcaa actctatggc cttgtagttc gccccttcca
     1261 caccgatccg gctgatggtg tgaagaatca ggtctttgcc gccgacccag ggctgaaggt
     1321 gaccgtgata aaccaaacga atggactccg gcacccgcat ccaggtctgc ccgaaggcca
     1381 tggcgaaggc gatgtccgta gctcccattc ccgtggcgaa agcgcccagg gccccgtagg
     1441 tacaggtatg ggaatctccc cccgcgacca cgtcccccgg caaaaccagt ccctgttcgg
     1501 gcagaaggac atgaacgatc cccgagcgcc ctacgtcgta gagcagagtc ccatactgga
     1561 aggcgaattc cctgaggacc cggctggtct ccgcagcatc cacattcgga gcaggcacgc
     1621 tatggtccag gaccagcacc actttggaag gattgaatac tctcctggcc cccatttttt
     1681 ggaactgccg caccgcaatc aagcccgttc catcggtggt catgaccaga tccacaggga
     1741 cgttaaggaa ttccccgggt ttgaccgatt tcctccccgt atgggcagcc aggatcttct
     1801 cggccagggt caacggacac tcctttttct acccggatag ccgccgataa acagcgcttt
     1861 gaagccagcg ttttcaataa tccgggcgct cagggcgtca tgggccctag gcatgaccag
     1921 aatttcgggg gcgagaatat gcttttcaag aagcgaagtc tttttcatcc cccctcccag
     1981 ggtgaaaaaa ggccgaattt gaatgtaaaa aaaagaaggc cctacttggt ttcaggcccc
     2041 cttggttaaa aaggaaaagt gtccaggtcg aaagccccgg gttcccttcc aatttttttc
     2101 tgaaagagga ctgacaaaaa gctggaatag tcccaagaat attggcaatt tttatattat
     2161 gatggatggt ttgtcaagaa tattttgagc cagaccagct tcaccggagg tgaagctgaa
     2221 aaaatcagcg ggcgatttca ggaaggtctt cagagagggc gttgccctgg aacgagatcc
     2281 ccgaggtcat ctctttccag aaaagatcgg cccgggtaaa agggaagaaa accaattgag
     2341 aatcttgcaa ttcgacccga caatctttga tccaggccct ggcttttcgg ttcgcctggg
     2401 ggatcaacgc ccccaggatc atcatcccca actcttgcgc atccttgatc cccaggcttc
     2461 ctcccgccgt gaggggatgc gaaccatctt cgaagcagcc gagcgggaca tccggctgta
     2521 agaaagtgag ccggctcccc agattatgga tggtttgggg attacggaat tttaccgccg
     2581 ggaagtagaa aaagattggc cggcgggact cgcgctcggg attgaagacc cgagggggcg
     2641 gggctaagcg gtaaagatcc gccatgcttg ccagcctctc gcccgatgac tccagggtgg
     2701 tccggcaccg ccagaagggt acccacagca ggtccttcgg aagacgttct ccggcgggcg
     2761 ggaccgccga ataattcatt tccgcccaat cccctccttt ttcccggaag agatggcggc
     2821 aggtagggca gaagtggaga acgctgaggg ggaaaaacgg aaatgcccag ccgcaattgg
     2881 ggcagcggaa ggggagaaag cgcagctcgc tgaactcgaa aggcttgcgg gtttcgttcc
     2941 cccgcaactt ggccaggatg ggagacccgt ccgggatcat ctgaatcgga ctctggccta
     3001 cggcatccag gaggagaatc tcgcagccct cttcatgcag gcattccaca aaccagaggg
     3061 ggaaatagat caccgaaaac tgaagcccta cgatcctttc caggttcacc tcggagatca
     3121 ttttctccgc ctggaagaag aaatcgacgt agcgcccgac ctgggcctgg accgcttcaa
     3181 ggggaaggtc cagggggagg aaggaatccc tcatctccag gtgctcgggg gaaaagggct
     3241 ggaggcgcag gacctgcggc cggaccccca gactgctcag cccaatctcc aggtcccaat
     3301 aggccgggat ggtatgatcc atcacccgga cgaggagaat tttgaaacgc tccatgggcg
     3361 gaggaacgcc cgcctcgctc tccattttca ggggtttcag cccaaaaacc catcggtaaa
     3421 ccgtcccctg gagacgccag aatggagcgt agaagaggag ggtttctccg attcgggggg
     3481 aacgccttcc cagcttcaaa aggtgttccc cggccagaga ctgggctttc ctcgggtcgg
     3541 aaatctgcgg agaaagcaca tagcgcagca ccccctcccg gccagcgacc aggagttcgg
     3601 tgccgcagaa ctggcagtga atgacttggg cttcctcgag gaaatcgatc gtcccgccgc
     3661 attgggggca gcggatgatg atgtgaactt tcgactcggc aggaatttcc atgggaaaga
     3721 tagtgtcagt atgagtgttc gtgtgagtaa aaactaaaat tgaatgacac gaattactga
     3781 ccctgacact ttttttatag tttttctccg cactgattgc agaagaccgc cccgggcaag
     3841 gcttgggtgc cgcatttgga gcaggtgata ttgggctttt ccactttggc cccgcagacc
     3901 atgcagaagt tagcctccag gggcaggtct ttcccgcagt ggagacactt gtttactttt
     3961 acgatctggg tgccgcagta aggacagaag cgcgcatcga tagagatctc gctgtggcac
     4021 ttgggacatg gaatcttctt ctcgctgggc atgctcccct cctgcatggt cttcattaac
     4081 atgcccggga gcatcatgcc caacccggcc cctacgccca ggcccatgcc cgagctggcc
     4141 gcggcgccag cggctcctcc tccttcggct ttggccgcat cccccaaggc cttggcggct
     4201 ttgtacttca ggaagtca
//
LOCUS       DASPRR010000013        12082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_3675, whole genome shotgun sequence.
ACCESSION   DASPRR010000013 DASPRR010000000
VERSION     DASPRR010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12082)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12082
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3675"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEL68_00550"
     CDS             <1..744
                     /locus_tag="VEL68_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34485.1"
                     /translation="AVALALLKNEKPDGYTVGNLSAAGILSQYMRKVPYDVMTDFTPI
                     MRYGDYTYGVVVRAEAPWKTFKELVDYAKASPGKVRYSTSGAGTFHHLAMEALAKQEG
                     IKWVHIPYKGGHEATTAILGGHVEVEACSSEWKPHVESGKLRLLATYNPTRLPKFPDA
                     PSWVELGYRVTASGYIGILGPKGMPRSIIEKLHGAFKLALEDPGFKKSMETYDMPVVY
                     RDPEGLGKDIKEFGEKWGKMIAELGIKQE"
     gene            749..1204
                     /locus_tag="VEL68_00555"
     CDS             749..1204
                     /locus_tag="VEL68_00555"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ34486.1"
                     /translation="MNNDQLSSTVWFILGLAVCLGSLQYKLGTPASPGTGFMPFLAGV
                     AICFFSGIGLIHSTFRRRQGEGWAPVLQGVFWKNAMVILISLLAYALLLVPLGFVITT
                     TLFIAFLLRAIVPQRWSVVIACSILTAVISYLIFEVWLKAQLPKGPLGI"
     gene            1208..2704
                     /locus_tag="VEL68_00560"
     CDS             1208..2704
                     /locus_tag="VEL68_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ34487.1"
                     /translation="MDFLNSALLGFQVAFQPINLFFCFAGVLTGTLVGVLPGLGPVAA
                     MSLLLPTTFHVSPVSAIIMLSGIYYGAMYGGSTTSILVNIPGEAASVVTCLDGYQMAR
                     QGRAGPALGISAFGSFIAGTLSIVGLMFIAPPLAAMALKFGPPEYFSLMIMGLTILTF
                     LASGPMWKALLMAAFGLFLGCMGMDNMTASYRFTFNILELSDGVGLVPVVMGLFGISE
                     VMLNVEQSMERTVFETKIQNLLPNLRDWADSFWPIIRGTILGFFLGVLPGGGAVISSF
                     VSYAVEKRISKHPEKFGTGAIEGVAAPESANNAATGGAFIPLLSLGIPANAVMALLLG
                     AFIIHGVQPGPLLVKEHPQLFWGTVTSMYLGNVMLLILNLPLIGLWVKILKVPYPILF
                     PLILLFCLIGCYSLNNSVTEVLIMVLFGLIGYGFKKFQYEAAPLVLALVLGPMMENSL
                     RQALLMSAGSPVIFFERPISAILMGTAIFLLIFPLVPKFRKKRPALGE"
     gene            2940..3923
                     /locus_tag="VEL68_00565"
     CDS             2940..3923
                     /locus_tag="VEL68_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXZ34488.1"
                     /translation="MKEKRKVVIATASWHQDEVRMMEGMGATVKIIPDKEDDLYYSEV
                     ASADGLVVGLLPVTRQLMEKAPRLMIVARHGVGYDSVDLKAATDLGIVVTNTPGVNSD
                     AVAEFTFGLLLAYIRQIPHSWELMRGGGWRKPQNLEAVDIRGKTLGIIGLGRIGSRVS
                     KIGNAFGLKVLACDPYISEEDFKNAGAQPASREEAIRQADYLTLHTPLSDETRKMIGA
                     KELSMMKKSALLINTSRGEVVDEDALIEAANKGTIAGAALDVFKDEPPKDKRIASHPR
                     ILVTAHLAGLTREAMYRTFIAAATQVVHALKGEKPPYALNQPKNPRYLKKG"
     gene            3995..4462
                     /locus_tag="VEL68_00570"
     CDS             3995..4462
                     /locus_tag="VEL68_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXZ34489.1"
                     /translation="MANPEEMMAKMEKTPEELAALIRGKNDKLLSQRPDGKNWAPNEI
                     ICHLRDTDELFLGRFQAIIAMDEPKFSPAAADRWAEDRQYLRNDVQEALAALRKRRGE
                     VLEFLRGLSPEQWERAGIHATRGRETLRDWVTLMANHDLNHLEQMKRALIGQP"
     gene            complement(4551..5207)
                     /locus_tag="VEL68_00575"
     CDS             complement(4551..5207)
                     /locus_tag="VEL68_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXZ34490.1"
                     /translation="MINLAELGELVKSRRSIRKFQDKPVPEETLLKALELATWAPNSG
                     NQQSWRFWIVTNKDLIQKIGDAVKAKTELMASWPEAKQFGETVARWRKTSDFFRGAPA
                     CIIVLMGKYSSIADQILHARGEKDPVAREIHSYRDTGASCLQTVAAACTYICLFLHYF
                     GLGTTWMAGPIQAKKEIEALLNVPPEWDFVDLIPVGYPAETPELRPRKPIQEVIRFFR
                     "
     gene            complement(5390..7108)
                     /gene="ilvD"
                     /locus_tag="VEL68_00580"
     CDS             complement(5390..7108)
                     /gene="ilvD"
                     /locus_tag="VEL68_00580"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXZ34491.1"
                     /translation="MKPKSSRIRKEWLQVDALCCGMDWDEADLKKPQILIEDVWGDSH
                     PGSVHLDSLAREASVGVFQEGGKPARFHGTDICDGWAMLHSGMNYILPSREVLCDLVE
                     VHGRVIPWDGIVAISSCDKSVPAHLMAIARLNFPAIHIPGGSNRVGPNMSHSLLVGET
                     ATRWRRGENVSREVRDYQLTGCPGAGACQFMGTASTMQCMSEALGMALPGSALIPASL
                     TAIGRLSRQAGRQIMELSRRGIKASDILTKEAFENAIKVHAAIGGSTNALLHLPAVAH
                     ELGIQIDAHLFDRYNREVPYLANVQPSGAYVNELFWYAGGIPRVQMELRDLLDLKVLT
                     VTGKTLGENLRRTEREGFFRRGAGYLANFRLQREDLIRRVKDSKSYGSIAVLKGNIAP
                     EGAVVKYAAVAAEMRTHEGPARVFDREEPAHEAIVRGKIKPGSVVVIRYEGPRGSGMP
                     EILSTTEALVTNPALHDTAIVTDGRFSGATRGPCIGHVSPEAAQGGPITLIEDGDLIR
                     IDIPKRILSVVGNRGKKMDPRAIQGLFEERRKRWKPPKLQHPSGILRRYATQATSAMK
                     GAYLEG"
     gene            complement(7498..8394)
                     /locus_tag="VEL68_00585"
     CDS             complement(7498..8394)
                     /locus_tag="VEL68_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXZ34492.1"
                     /translation="MNRIGFVGLGTMGKPMARNLMKGGYSLIVHDLNWEPVKELGSAG
                     AKEAFSPKEVGQAAEVVFTSLTNDQVVEEVVLGKEGLLEGMKKGSVLVETSTISPLTV
                     QKIASKLNSAGIEILDSPLSGGEIGAQQATMSIMVGGKAEVFEKVLPLLQKMGKNITH
                     IGSQGAGQIAKAANQIIVALTIEAVCEALIFAQKAGADPAKVRQALLGGFAQSRILEE
                     HGQRMLDRNFKPGGKAIFHQKDIGIVLAVAREKGIYMPGAMLVMDLWNAMGAHGLLQE
                     DHTAMLKVLEKMSHTEVRPGEK"
     gene            9418..9966
                     /locus_tag="VEL68_00590"
     CDS             9418..9966
                     /locus_tag="VEL68_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008512483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ34493.1"
                     /translation="MVEIRFHGRGGQGAVIASKILASALFKEGKYAQAFPAFGAERRG
                     APVMAFTRFDQKAITRRSMVYEPNHVVVLDEPILDLVDVTSGLKDGGWILINSSKSPS
                     DFPQFKKYKVATVDANRIAAENKLGTTTAPVVNTVILGAFSKISGLVGLEAVIDGIKE
                     YVPSKPEANAAAAKAAFEQANA"
     gene            10039..11832
                     /locus_tag="VEL68_00595"
     CDS             10039..11832
                     /locus_tag="VEL68_00595"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXZ34494.1"
                     /translation="MQKKPATKKAVVKKPAVRPRKPIQPKSSLPPMSLSFEGTLATKT
                     GTWRYLTPAYLNKLAPCNEACPAGEDVEAAMVLSGQADYLGAWEKIIQENPLPRVCGR
                     VCFHPCEGSCNRKEYDEPTAINALERFVGDTAYQSGKRLPPPAEKKKEKVAVIGSGPA
                     GLSCAYHLASLGYGVTLFEALPQLGGMLRYGIPAYRLPKAILDQEIDQILALGVESRT
                     NCRVGKDVNWEELRKFDAIFVAAGAWRSLPLNVPGEDSAGVMSGVTFLRKVNSGEKVD
                     LGERVAILGGGNTAMDAARSALRLGVKPLVIYRRTKEEMPAWEEEVSEAEEERIEFIF
                     LTSPVKVMTEHGRVKGIECIKNILGPPGKDGRREPRPIENSNFSLPVDSVISAIGEAP
                     DLSFLPTEMQKSKGDLPVDETGATSQKKVFAGGDVVAQPRTVSWAIGSGKKAAMAIDA
                     TLRGKNVAEAIRLASWGGKGSLSMARYRSKGGDGVAQEVVKFSNLNTAYFPRQNRKPK
                     DRLTVDQQTRDFSEINHGLSPVTALFEAKRCFNCGVCNLCDNCFLFCPDLAISAWPNR
                     QGYEINYDYCKGCCICVEECPRGAISIEVKK"
     gene            11829..>12082
                     /gene="porA"
                     /locus_tag="VEL68_00600"
     CDS             11829..>12082
                     /gene="porA"
                     /locus_tag="VEL68_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HXZ34495.1"
                     /translation="MKKLLTGNSAVSLGVMLCRAEVISAYPITPQTTIVEELSEIVAD
                     GRLKARFIRVESEHSALACVAAASASGARVFTATSSHGLA"
BASE COUNT         2577 a         3438 c         3405 g         2662 t
ORIGIN      
        1 gccgtggctc tagccctttt gaagaatgag aagcccgacg gatacacggt cggaaacctt
       61 tccgccgcgg gaattctgag ccaatacatg cggaaggtcc cctatgacgt gatgaccgat
      121 tttaccccca tcatgaggta cggggattac acctacgggg tggtcgttcg agcggaagcc
      181 ccctggaaaa ccttcaaaga gctggtcgat tacgcgaagg ccagccccgg caaggtccga
      241 tacagcacat ccggagccgg aactttccat cacctagcca tggaggccct ggccaaacag
      301 gaagggatca aatgggtcca cattccctac aaagggggcc acgaggcgac cacggccatc
      361 ctcggagggc atgtggaggt cgaagcctgc agttccgaat ggaagcccca tgtggaaagc
      421 ggcaaattga ggcttctggc cacctataac cccacccgct tacccaagtt tcccgatgcc
      481 ccctcctggg ttgaactggg gtacagagtc accgcgagcg ggtacatcgg gatcctcggc
      541 ccgaagggaa tgccccgctc catcatagaa aagctccacg gggcttttaa actcgcgctg
      601 gaagacccag gtttcaaaaa atccatggaa acgtacgata tgcccgtcgt ctatcgcgac
      661 cccgaaggcc tgggcaaaga catcaaggag ttcggcgaaa aatggggtaa aatgatcgcc
      721 gagttgggga tcaaacagga gtgaagcgtt gaataacgac cagttgagca gtacggtctg
      781 gttcattctg ggattggcgg tgtgcctggg ttccctgcag tataaactgg gaaccccggc
      841 atcgccgggc accgggttca tgcccttcct ggccggggtg gccatctgtt tcttttcggg
      901 aatcgggctg atccattcga ccttccgtcg aagacaaggg gaaggatggg cccccgtgct
      961 ccagggtgtt ttctggaaaa atgcgatggt catcctgatc tccctgctgg cttatgctct
     1021 tctgctggtt cccttgggtt tcgttatcac cacgactctg tttatcgcct ttttgctccg
     1081 ggccattgtt ccccagcggt ggtccgttgt catagcctgc agcatcctga cggcggtgat
     1141 ctcttatctc attttcgaag tctggttgaa ggcccagcta cccaagggcc ccttgggaat
     1201 ttgagctatg gattttctaa atagtgccct gctgggattc caggtggctt tccagcccat
     1261 aaatctcttc ttctgcttcg ccggggtcct caccggcacg ctggtgggag tgcttcccgg
     1321 cctgggtccg gttgccgcca tgtctcttct tctgccgacg accttccatg tctccccggt
     1381 ctcggcaatc atcatgcttt ccggaatcta ttacggcgcc atgtacgggg gttccaccac
     1441 ctcgatcctg gtcaatattc ccggagaggc agcctccgtc gtcacctgcc tggacggata
     1501 tcagatggcc cgccagggaa gagccggccc ggccctgggg atttctgctt tcgggtcctt
     1561 catcgccggc accctctcca tcgtgggact tatgttcatt gctcctccgc tggctgccat
     1621 ggccctgaaa ttcggcccgc ccgagtactt ttcattgatg attatgggtc ttaccatcct
     1681 caccttcctg gccagcgggc ccatgtggaa ggccctgctc atggccgcgt tcggcctatt
     1741 cctgggctgc atggggatgg ataacatgac cgcttcctat cgtttcactt ttaatatcct
     1801 ggagctctct gacggggtag gcctggtgcc ggtggttatg gggttgtttg ggatttctga
     1861 agtcatgttg aatgtggaac aatccatgga gcggactgta tttgaaacca agattcaaaa
     1921 tctccttcct aacctgaggg actgggcgga ttccttttgg ccgatcattc ggggaacgat
     1981 tctgggattt tttcttgggg tcttgcccgg gggaggagct gtcatttcct ccttcgtttc
     2041 ctatgcggta gagaagagga tttccaaaca tccagagaaa ttcgggaccg gagccatcga
     2101 aggagtggct gcgccggagt cggccaacaa cgcggccacg ggcggtgcct ttattcccct
     2161 cctctccctg ggcattccgg ccaatgcagt catggcgctt ctcctgggcg ctttcatcat
     2221 ccatggagtc cagcccggcc ccctgctggt gaaagaacat ccccagcttt tctggggtac
     2281 agtgaccagc atgtacctgg gaaacgtcat gctcctaatc ctgaaccttc ctctgatcgg
     2341 gttgtgggtc aaaatcctca aggtccccta cccgatcctt ttcccgctga tcctcctctt
     2401 ctgcctgatc ggctgctaca gcttgaacaa tagtgtcacc gaggtgttga tcatggttct
     2461 cttcgggctg attgggtacg ggttcaaaaa attccagtac gaggccgcgc ccttggtctt
     2521 ggccctggtc ctgggaccga tgatggaaaa cagcctgcgc caggccctgc tcatgtctgc
     2581 gggaagcccg gtcattttct ttgagcggcc catatcggcg attctcatgg gaacagcgat
     2641 ttttctctta atctttccgc ttgttcccaa gttcaggaag aaaagaccag ccctgggaga
     2701 ataaatccca atcccgccac aggcggggcc gaatcctaaa ttcgggagga aacaccgatc
     2761 ttccagcacc aaattccaat gcccgattaa tccaagcacc ggataggggg ggttgagttt
     2821 gggatatcgg gattggagga attttggggg tgggattttg tttattctct tctaggcaat
     2881 tcacggaacc atacgtacaa tgagaacagg tttggaaaat aaagatcgag aggatggaca
     2941 tgaaggaaaa gagaaaagtt gtcattgcca cggcatcctg gcaccaggac gaggtcagga
     3001 tgatggaagg aatgggggcc acggtgaaaa tcatcccgga caaagaggac gatctctatt
     3061 actccgaagt cgcctcggcc gacggcctgg ttgtcgggct tttgccagtt acccggcagc
     3121 tcatggagaa ggctccccgg ttgatgatcg tggctcgcca cggcgtgggt tatgacagtg
     3181 tggatttaaa agccgccacc gatttgggaa tcgtggtcac caataccccg ggggtgaatt
     3241 ccgatgccgt ggccgagttt actttcggcc tcctcctggc atacatccgg cagattcctc
     3301 attcctggga attaatgaga ggagggggct ggcgaaagcc ccaaaacctg gaagcggtgg
     3361 acatccgggg aaaaactctc gggattatcg gactggggag gattgggagc cgggtcagca
     3421 agataggaaa cgcattcgga ctaaaagttt tggcttgcga cccctacatc tcggaagagg
     3481 atttcaaaaa cgccggcgcc cagcctgctt ccagggagga agccattcgc caggcagact
     3541 acctgaccct ccacactcct ttgagcgatg aaacccggaa gatgatcggt gctaaggaac
     3601 tttccatgat gaagaagtcc gccctcctga tcaacacctc ccgaggagaa gtggtggatg
     3661 aggacgcttt gatcgaggcc gccaacaagg ggaccatcgc cggggcggcc ctggacgtct
     3721 ttaaggacga accgccgaaa gacaagagga ttgccagtca tccccggatt ctggttaccg
     3781 ctcacctggc cgggctgacc cgggaagcca tgtaccggac ctttatcgcc gcagccacgc
     3841 aggtggtgca tgcgctgaag ggagaaaagc ctccttacgc tttgaaccaa ccgaagaatc
     3901 cccggtacct gaagaagggg taaaaagaat tgtaggggtg ggattaaaac ctgcccctac
     3961 atccgtcaaa atgccaaggg ataaaggaga gaaaatggca aaccctgaag agatgatggc
     4021 caagatggaa aagaccccag aagaattagc tgctctcatt cggggtaaga atgacaagct
     4081 tctatcccag cgtccggacg gaaagaactg ggcgcccaat gagatcatct gccacctgcg
     4141 ggacacggac gaactcttcc tgggccgttt ccaggcaatc atcgcaatgg acgagcccaa
     4201 gttctccccg gcggctgcgg accgctgggc tgaagatcgg cagtacctga ggaatgacgt
     4261 ccaggaggcc ctcgccgccc tccggaaacg gcggggagag gtcctggaat tcctgcgggg
     4321 gctgagcccg gagcagtggg agcgggccgg gattcatgcg acccgggggc gggaaacgct
     4381 acgggactgg gtcaccctca tggccaacca cgatctcaac catctggaac agatgaagcg
     4441 ggctttgatc ggccagccat agaggcgggt ttcaacccaa cccctgccgc aggatgaaag
     4501 agcaatccgg taggggcaat ttatgaattg cccctactcc gccgttgaaa tcaccggaag
     4561 aaccggatca cttcctggat cggcttcctc ggcctgagtt caggagtctc cgccgggtat
     4621 cccaccggaa tcaggtctac gaaatcccac tcgggcggca cattcagaag cgcttcgatt
     4681 tccttcttgg cctgaatcgg gccggccatc caggtcgtgc ccaacccgaa ataatgcagg
     4741 aaaaggcaga tgtacgtgca ggctgcagcc accgtctgca ggcaggaggc tccggtgtcg
     4801 cggtaggagt gaatttcccg ggctacgggg tccttctctc cccgggcgtg caggatctgg
     4861 tcggcaatgc ttgaatattt tcccatgaga actatgatgc aggccggcgc cccccggaag
     4921 aagtcggatg tcttccgcca cctcgccaca gtctccccga attgcttggc ttccggccaa
     4981 gaggccatga gttcggtctt ggccttcacc gcatccccta tcttctggat taaatccttg
     5041 ttggtaacga tccagaacct ccaggattgt tgatttccac tgttgggcgc ccaggtagcc
     5101 agctccaggg ccttgagcag ggtctcctcc ggtaccggtt tgtcctggaa cttgcgaatg
     5161 gagcggcgac tcttaaccag ctctccgagt tcagccaaat tgatcatata acatcctcct
     5221 ctagctgaaa ttatgcctat tcggaaattc acgaaagact cctcggcgtt tcaccaaggc
     5281 gtgaaaagaa aataaccgat gtggggcgat aaatcaagga aggagttgcc gcggcaggaa
     5341 gaaggggcga cccccggcta aggggagcgc acccttcccc gaaggtctct tagccttcca
     5401 aataagctcc tttcatggcc gatgtagctt gcgtcgcata tcggcggaga attcctgaag
     5461 ggtgctgtag tttgggcggt ttccatctct ttctccgttc ctcgaaaaga ccctgaatgg
     5521 cccgggggtc catcttcttt cccctatttc ccacgacgga aagaatccgc ttgggaatat
     5581 cgatccggat gaggtctcca tcttcgatca gagtgatggg gcctccttga gcggcttcgg
     5641 gggaaacgtg cccgatgcag ggaccccggg tggcgccgga aaatcttccg tcggtcacga
     5701 tcgccgtgtc atggagggcg gggttggtta ccagggcctc ggtggtggac aggatctcgg
     5761 gcatcccact tccccggggc ccttcgtagc ggatgaccac cacagacccg ggcttgattt
     5821 ttccgcggac gatggcctcg tgggctggct cctcccggtc gaagacccgt gcaggccctt
     5881 catgggtgcg catttccgcg gccacggctg cgtacttgac caccgcccct tcgggggcga
     5941 tgttaccctt caggaccgcg atggagccgt aggatttgct gtctttcacc ctgcggatca
     6001 ggtcttccct ctggagtcta aaattggcta gatacccggc ccccctgcgg aagaaccctt
     6061 ccctttccgt ccgccgcaga ttctctccca gagttttccc ggtcacggtt aataccttta
     6121 agtccaagag gtctctcagc tccatctgta cccgcggaat tccgccggca taccagaaga
     6181 gttcattcac gtaagcgccc gaaggctgga cattggcaag gtaagggacc tcccggttat
     6241 agcggtcgaa gaggtgggca tcgatctgga ttcccagctc gtgggccacc gccggcaggt
     6301 gcaggagggc gttggtggac ccgccgatgg ccgcatggac cttgatggcg ttttcaaagg
     6361 cctcttttgt aagaatgtcc gatgctttga tccctctcct ggacagttcc atgatctgac
     6421 ggccggcctg gcgggataaa cggccgatgg ccgtaaggct cgcaggaatc agagcggacc
     6481 cgggcaaggc catgcccagg gcttcggaca tgcactgcat ggtgctcgcc gtccccatga
     6541 actggcaggc tcccgctccc gggcagccgg tcagttggta gtcccggacc tcccgggaaa
     6601 cattctctcc ccggcgccac ctggttgccg tctcacccac caggaggcta tgcgacatgt
     6661 tgggtccgac ccggttgctc ccgcccggga tgtgaatcgc gggaaaattc aggcgggcga
     6721 tggccatcaa atgggccgga acggacttat cgcatgagga gatcgcgacg attccgtccc
     6781 aggggattac tcgaccgtgg acctcgacca ggtcacatag gacctcccgg gaaggaagga
     6841 tataattcat cccgctgtgc agcatggccc acccgtcgca gatatcggtc ccgtggaagc
     6901 gggccggttt tcccccttcc tggaatactc cgacggaggc ttcccgggcc aggctgtcca
     6961 gatggacgct ccccgggtga ctatctcccc acacatcttc aattagaatc tggggctttt
     7021 tcaggtctgc ctcgtcccag tccatgccgc agcagagggc gtccacctgc aaccattctt
     7081 tccgtatcct gctgcttttc ggtttcataa atctcctatt tttcttggtt cttctcccaa
     7141 tggcgttggt gatttcagtc gattttcaat aggctgccat actcgctccc gtggccgggg
     7201 agatttggcc aggcccaggg ggtgcgttcc gcccatggcg ggacagaatg aggcacgagt
     7261 tctttcatcg gttcaggtcg gaaaattctt ttgttttcag gacctgagcc aagattcggt
     7321 tttccgaaag ggcatcgcgg acaggcgctt tgcggaaccc aagcaccctc tccggaatcc
     7381 gggtatttcc cgccgattct gtcccgccat gggcggaacg caccccctgg gctgagaata
     7441 tttctgcttt cctcccaccc actcgattgg gagaagagcc tttttcttta ccggaagcta
     7501 tttttccccc ggacggacct cggtatggga catcttctcc aggaccttca gcatggcagt
     7561 atggtcttcc tgaagcaggc catgggctcc catggcgttc cagaggtcca tcaccagcat
     7621 ggcgccaggc atgtaaattc ccttttctct ggcgaccgct aacacaatac caatatcctt
     7681 ttggtggaaa atggccttgc cccccggttt gaaattacgg tccaacatcc tctggccgtg
     7741 ctcttccaga atgcggctct gggcaaaccc tcccagcagc gcttggcgca ccttagctgg
     7801 gtcggccccg gctttttggg caaagatcag ggcttcgcag accgcctcaa tggtgagggc
     7861 cacaatgatc tgattggcgg ctttggcaat ctgccccgcc ccctggcttc cgatgtgggt
     7921 gatgtttttt cccatctttt gaaggagcgg gagcaccttc tcgaaaacct cggcttttcc
     7981 ccccaccatg atggacatgg tagcttgctg agccccgatt tcacctccac tcaaagggct
     8041 gtcgagaatt tcgattcccg cggagttcag cttggaggcg attttctgca cggtcaaggg
     8101 agagatcgtg ctggtctcga ccaggacaga accttttttc atcccctcca ggagaccttc
     8161 tttccccaag acgacttctt cgacaacttg atcattcgtc aaagaagtaa aaaccacttc
     8221 cgctgcctgc cccacctctt tcggggagaa tgcttccttt gcccccgcag agcccagctc
     8281 tttcaccggc tcccagttca gatcgtggac gatcaaggaa tagccgccct tcatcaaatt
     8341 tctggccatg ggtttcccca tggttcccag ccccacaaag ccaatccgat tcatcttcat
     8401 ctttctttca cccctcaaaa atctttctaa aattctctac agggttttcc cgcgaagaga
     8461 aattgacgat ctcgtaaaaa gtctcgtaaa tcgccacccc ggcgaacccc ggaacaggtc
     8521 cggggcaggc gccggggtcc agaagttgtc tcgacgaaag tcgggaacca tctccaagac
     8581 cggaccccgg ttttcaccgg gaatcctaga ttccggcttt cgcctgaatg acgaacttta
     8641 taggattccg gtttttgcga ctccatcaaa attcagttcg gcaatgaatg cctcgattct
     8701 tataatataa aaccattctt gtcaagtatc cttgatttct tgcccacccc cagttcttaa
     8761 aaaagagtcc ttcgaaaata agatctctgg aaaggagggg ggatctcatg gggttcaggg
     8821 tggagaaatt tggatcatca tcctgaggcg gtggttcttc catgggatcg ggcctgtcta
     8881 gggggggatt gaaacggaaa aatgctttgg ttctcttttc tgcttggagg tatctcctct
     8941 caggcctctc cctgcggaac agggggagga gtttcatgga aggataggca cccgccgagc
     9001 ctgatgatcc aaattcctcc accctgaacc ccatcaagca cgaaaactgt gggtgggcaa
     9061 gtccttgatt tcggtttgtc tggagaccat gaaaggccgg ggaaatcacg ctgcgagatc
     9121 gcccaaaggg ggtggtagga ttgaggaaaa gcattgacag ggggtctgtt tgagattata
     9181 agatgaaact gggtttggcg atgggaaaag ggcttcatcc tttttcctct ccggaaaatc
     9241 cgtttgggtt cggtggcggg tttgaggcca cagttgggcg atcttcgtac tgtttcccct
     9301 ttctcggccc ctctggaata agaccttttt taatgcgaat cgacaggaaa agaggctaag
     9361 tgtcggacct tttcagtgga ctgcgtcatg cagcccgaag gatagaggag atcaaagatg
     9421 gtggaaatac gttttcacgg aaggggcgga cagggggcgg tcatcgcctc caaaatcctg
     9481 gcttccgcgc ttttcaagga aggaaagtac gcccaggcct ttcccgcctt cggtgccgag
     9541 aggcggggtg ccccggtcat ggcttttacc cggtttgacc aaaaagccat cacccggcgg
     9601 tccatggtct atgagccaaa ccatgtggtg gttctggacg aacccatcct cgacctcgtc
     9661 gatgtgacct ccggccttaa ggatggcggc tggatcctga tcaacagttc caagtcccct
     9721 tcggattttc cccagttcaa aaaatataaa gtggctaccg tcgatgccaa ccgaattgcc
     9781 gcggagaaca aattgggaac gactacggcc ccagtggtga acacggttat ccttggggct
     9841 ttttccaaaa tcagcgggtt ggtggggctg gaggcggtta tcgatggaat caaagaatac
     9901 gtcccctcga aacccgaggc caatgcggct gcggccaaag cggcctttga acaggcgaat
     9961 gcttaaacag gttcggagga ggtaagggcg accggccggt cgcccctacg aactccggac
    10021 ttcctatagg agtcgaaaat gcagaagaaa ccggccacga aaaaagccgt ggtaaaaaag
    10081 ccggcggtcc gtccgagaaa accaatccag cccaagtcct ctctcccccc catgtccctc
    10141 tctttcgagg gaaccctcgc taccaagacc ggaacctggc gctatctcac ccccgcttat
    10201 ctgaacaagc tggctccctg taatgaagcc tgtccggcgg gcgaagatgt ggaggcggcc
    10261 atggtcctgt ccggacaggc agattacctc ggggcgtggg aaaaaatcat ccaggaaaat
    10321 cccctgcccc gggtttgcgg gcgggtctgc ttccacccct gtgagggatc ctgcaaccgg
    10381 aaagagtatg atgaacccac ggccatcaac gctttggagc gattcgtcgg cgatactgcc
    10441 taccaatccg gaaagcgcct ccctcccccc gcagaaaaga aaaaggaaaa agtggcggtc
    10501 ataggctcag ggccagctgg cctttcctgc gcctaccatc tggcttccct gggttatggg
    10561 gtcactctct ttgaggctct tccccagctg gggggtatgc tccggtacgg aatcccagcc
    10621 tatcgcctgc ccaaagccat tctggaccag gaaatcgatc aaatattggc tctgggtgtg
    10681 gaatccagga cgaactgccg ggtgggcaaa gatgtgaatt gggaagaatt gagaaaattc
    10741 gacgccatct tcgtggccgc aggggcctgg agaagcctgc ccctgaatgt tcccggggaa
    10801 gattccgccg gagtcatgtc tggagtgacc ttcctaagga aagtcaattc cggagagaaa
    10861 gtggacctgg gggagagagt ggcgatcctc ggcgggggaa acacggccat ggatgcggcc
    10921 cgttcagccc tgcgcctggg agtaaaacct ctggtcatct accgccgaac gaaggaagag
    10981 atgcccgcct gggaagagga ggtctccgag gcggaagaag agaggatcga attcatcttc
    11041 ctgacctccc cggtgaaagt tatgaccgaa cacggaaggg tcaaaggaat tgaatgcatc
    11101 aaaaatattc tcggccctcc tggaaaagac gggcgaagag aaccccgccc gatcgagaat
    11161 tccaatttta gccttcccgt ggattcggtg atctcggcca tcggggaagc tccggacctg
    11221 tccttcctcc ctacggagat gcagaagtcc aagggggacc tcccagtcga tgaaaccgga
    11281 gctacgagcc agaagaaggt ctttgccggg ggcgatgtcg tggcccagcc ccgtaccgtt
    11341 tcctgggcca ttggttcggg gaaaaaagcg gccatggcca tcgatgccac cctgcgggga
    11401 aagaacgtgg ccgaagccat ccgcctggcc agctggggag ggaagggaag cctttccatg
    11461 gcccgatatc ggagcaaagg gggggatgga gtcgcccagg aggtagtgaa attttcgaac
    11521 ctgaacacgg cctactttcc ccgtcaaaat cgcaaaccca aagaccgcct gaccgtggat
    11581 cagcagaccc gggacttctc cgaaatcaac cacggattat ctccggtcac cgccctcttt
    11641 gaagccaagc gttgcttcaa ctgcggggtg tgcaacctct gcgacaactg tttcctcttc
    11701 tgccccgatc tggccatctc cgcttggccg aaccggcagg gatacgagat caactacgat
    11761 tactgcaagg gatgctgcat ttgcgtggaa gaatgccccc gcggagccat ttccatcgag
    11821 gtgaagaaat gaaaaagttg ctcaccggaa actcagctgt ctccctgggg gtcatgctct
    11881 gccgggccga agtcatctcg gcttacccca ttacgcccca gaccaccatt gtcgaagagc
    11941 tctcggaaat cgtggccgac ggtcggctaa aggccaggtt cattcgcgtg gaatcggagc
    12001 attccgccct ggcctgcgtg gccgcagcct cggccagcgg cgcccgggtc ttcaccgcca
    12061 cttcttccca tggactggcc ta
//
LOCUS       DASPRR010000014         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_487072, whole genome shotgun sequence.
ACCESSION   DASPRR010000014 DASPRR010000000
VERSION     DASPRR010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487072"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VEL68_00605"
     CDS             complement(<1..1153)
                     /locus_tag="VEL68_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ34496.1"
                     /translation="MSKEITRRKFIEDVAKYCGAGIAAVGLIGPTRAHAVADLDTPAH
                     EGIWRMRDSQLMQYGEFIKNAPLPKDYFEKPKDKLVDGGAPVNWSHLHPNYYGEGNKS
                     LVGLIMATELDKDLKGDIKKAMKLIGGFEKSLKKSDKILIKPNMNTGDPWPQGGTDHE
                     FLKALISVLKDEGYTNLTVADTCGPWGPTDRVIKLLGLDVACKETNTPVKTWENEQWM
                     NIRNEKAEFLGRIGGGDGTVAYPADLRNYDKVIYTPVMKTHFLGGITQSLKLSVGILH
                     RADRGGQLHNFNHIFVAEQAAEINLPFQPDLIIMDGRRSLISGGPSHGEQVKPGVILA
                     SGDQVANDVMGIRIMQEWYPNMAANKIGMYAWYQKQIAQAVKLGIGYAKK"
     gene            1784..2218
                     /locus_tag="VEL68_00610"
     CDS             1784..2218
                     /locus_tag="VEL68_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXZ34497.1"
                     /translation="MKKKLLLLPGTFSICRLESNASIPEWALSGGFSSITRTAEELSV
                     VCAQDQVPPGIQRQDGWKALQVEGPLDFSLTGVLASLTDPLAKEGISVFAISTYDTDY
                     LLVKEEQLEGAIQALRGEGYEINKDRGRGGFETRPYGVRSKK"
     gene            2290..3474
                     /locus_tag="VEL68_00615"
     CDS             2290..3474
                     /locus_tag="VEL68_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ34498.1"
                     /translation="MEFQVAQRTESFQESVIRKMTRVCQQYNGINLAQGFPEKDPPEE
                     VVQEALRAIQQGNNQYSITWGLPALREAVAKKVSRFAGLKVDPEREVTITCGSSEAIV
                     ASLMALVNPGEKVVVFEPFYENYLPGLTLCGGIPVFIRLRDPNWALDPKELKKAFSQR
                     PKAVILNTPANPSGKVFSREEMELIARCCQETGTYVITDEIYEHILYDGLKHTNMAGL
                     PGMWERTVTISGISKTYSATGWRVGYVIAPSGLMAAVRKCHDYMVVAAPTPFQMGAVL
                     AMGLPDSYYGELQEFYRERRDFLLKVFEDLSIPVFRPAGAYYMMIRINGLGCGDDEAF
                     AMALVEKAGLAVVPGSSFYHRPQDGRDKVRFCFAKKWETLRQVKERMEGFLKNREKGC
                     RL"
     gene            complement(3471..>4113)
                     /locus_tag="VEL68_00620"
     CDS             complement(3471..>4113)
                     /locus_tag="VEL68_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXZ34499.1"
                     /translation="LCRRISRGKTFHAILEMGKMVAIIATAFFLSQGYFFPSAQSSQT
                     ETFKVSEVREDFSPPKASTSDPNVKEILNLAAMIRFITNEQLSEGKALRYAGLIFHAS
                     KRYGVNPLEIIALITAESRFKEGSINSKTGDYGLGQVNWVHWGKDLGLTHQDLLDPAI
                     NIYMTCHIFNYFGKDFGKYNRGHGVKSRAYVLNIKGIISSLNAFAQMNLEEVS"
BASE COUNT          905 a         1081 c         1157 g          970 t
ORIGIN      
        1 gtttcttggc atagccgatg ccgagcttca ctgcctgggc gatctgcttc tgataccagg
       61 cgtacatgcc gatcttgttg gcggccatgt tcgggtacca ttcctgcatg atccggatgc
      121 ccatgacgtc gttggccacc tgatcgccgg aggccaggat tacgcccggc ttgacctgct
      181 cgccgtggga agggccgccg ctgatgagcg accttcttcc atccatgatg atcaggtcgg
      241 gctggaaggg gaggttgatc tccgccgcct gttcggccac gaagatgtgg ttgaagttgt
      301 ggagctggcc cccgcggtca gcccggtgca ggattcccac ggagagcttc aggctctggg
      361 taattccgcc gaggaagtga gtcttcatga cgggggtgta gatgactttg tcgtaatttc
      421 tcaggtctgc agggtaggcc accgtgccgt ctccgcctcc gatccggccc aggaattcgg
      481 ctttctcatt gcggatgttc atccactgct cgttctccca agttttgacc ggggtattgg
      541 tctccttgca ggccacgtcg agacccagca gtttgatcac ccggtccgtg gggccccagg
      601 ggccgcaagt atcggccacg gtcaggttgg tatagccttc gtctttcagg acggagatca
      661 gggccttcag aaattcatgg tcagtgcctc cctgcggcca ggggtcgccg gtgttcatgt
      721 tgggcttgat gagaattttg tccgattttt tcagggactt ctcaaagccg ccgatgagct
      781 tcatggcctt cttgatgtcc cctttcaggt ccttatccag ctccgtggcc atgatcagtc
      841 cgaccaggga tttattgcct tctccatagt aattggggtg gagatgggac cagtttaccg
      901 gggccccccc gtcgaccaat ttgtccttcg gcttttcaaa ataatccttg ggaagagggg
      961 catttttgat aaattccccg tactgcatca actggctgtc ccgcattctc caaatgcctt
     1021 catgggccgg tgtgtcgagg tcggccactg cgtgggccct ggtcgggccg ataagaccca
     1081 cagcagcaat tcccgccccg cagtacttgg ccacgtcctc gatgaacttc ctccgggtga
     1141 tttccttgct catttccctt tctcctttct agagttaagg aattttagag ttggatgatg
     1201 gagatacaga agtctctctc gccccttaaa agccgtcaat ctcccccctt tctatattga
     1261 aatgaccctc gagagttggg cagtccaccg ataaaaacct aatccataag tatcctgttc
     1321 ggaatttgga agcataaatt tggagtacaa atacattttg agcttaattt aacctttttg
     1381 gcaaatttgt caagaaattt tttcttgagc gttttactcg gtaatttgac gatctgaata
     1441 tagattggcc atcttatgca tcgatatggg cttaagggtc attttgctta ttttggaaag
     1501 caggcggcta tgataagcta ggctttaaac ggtgcgttcc gggaacatgc ctacattctc
     1561 ttctctccct cgaaggggaa ggtgtgaaaa tattacgaag aggccccctc accctaccct
     1621 ctcccgccag gggagggggt aaatatattg aaatacgaag agaacccccc tccccctcga
     1681 cgggggaggg acagggtggg ggtgaaaagg cgcgaaaagg atttttcgga tgaacactcg
     1741 ctcacccggg aaggtccggg gtaaaagaaa aggaacgagg aagttgaaaa agaaactcct
     1801 cttgttgccc ggaacctttt ccatctgccg tctggaaagc aatgcctcga ttccggaatg
     1861 ggcgttgagc ggcgggtttt cttctatcac ccggacggct gaagagcttt ccgtggtttg
     1921 tgcccaggac caggttcctc caggaattca gaggcaagat ggatggaaag cgctgcaggt
     1981 cgaggggccc ctggattttt ccctgaccgg agtcctcgcg tctctcaccg accccttggc
     2041 caaagagggg atcagtgtat ttgcaatctc cacctatgac acggattatt tgttggtgaa
     2101 ggaagaacag ctggagggag cgatccaggc actgcgggga gaaggatatg agataaacaa
     2161 agatcgcggt aggggcgggt ttgaaacccg cccgtacgga gttcggagta aaaaataaag
     2221 gctccgaact ccgcacgggc gaccaaccag tcgcccctac aagctttgaa tttaaatcag
     2281 gagcctagga tggaatttca ggtagcccaa cggaccgagt cattccagga atcggtcatc
     2341 cgcaagatga cccgggtgtg ccagcagtac aatgggatca acctggccca aggatttccc
     2401 gagaaggacc cccccgaaga agtggttcaa gaggccctca gagccattca gcaggggaac
     2461 aaccagtact cgatcacctg gggcctgccg gccctgcggg aagcggtggc gaagaaagtc
     2521 tcccgtttcg ccggcctgaa ggtagacccg gaaagggagg tcactatcac ctgcggcagc
     2581 agcgaagcga tcgtggcctc cctgatggcc ctggtgaatc cgggcgaaaa ggtcgtcgtc
     2641 tttgaaccat tctatgagaa ttatctcccc gggttgactc tctgcggtgg gattccggtg
     2701 ttcatccggt tgcgggaccc gaactgggcc ttggacccga aagaattgaa gaaggccttc
     2761 tcccagcgtc ccaaggcggt gatcctgaac accccggcca atccttcggg caaagtcttt
     2821 tctcgggagg agatggagtt aattgcccgg tgttgccagg agaccggaac ctacgtcatc
     2881 accgacgaga tctacgagca cattctttac gacgggctca agcataccaa tatggccggc
     2941 ctacccggca tgtgggagag gacggtcacc atcagcggca tctccaagac ctacagcgcc
     3001 acgggatgga gggtgggcta tgtaatcgct ccttccggcc tcatggcggc cgtccgcaaa
     3061 tgccacgact acatggtcgt cgccgccccg acccctttcc agatgggagc ggtcttggcc
     3121 atgggccttc ccgattctta ctacggggaa ctccaggaat tctaccggga gcgccgggat
     3181 ttcctgctga aggtttttga ggacctttcc attccggtct ttcggccagc gggggcttac
     3241 tacatgatga tccggatcaa cggactgggc tgcggggatg atgaagcctt cgccatggcc
     3301 ctggtggaaa aggcaggact ggccgtggtc ccgggttcca gcttctacca ccgaccccaa
     3361 gacggccggg acaaggtccg cttctgcttt gccaaaaagt gggagactct tcggcaggtc
     3421 aaagaaagaa tggagggatt cttaaaaaat agagaaaagg gatgccgtct ttaagaaacc
     3481 tcttccaggt tcatttgcgc gaaagcattc aacgaggaaa taatgccttt gatgttcagc
     3541 acgtaggccc tggatttgac cccatggccc cgattatatt tcccgaaatc cttcccgaaa
     3601 taattaaata tatggcaggt catatagatg ttgatcgcgg ggtccaggag gtcctgatgg
     3661 gtcaggccta aatctttgcc ccagtgcacc caattgacct ggcccagccc gtaatcgccg
     3721 gttttggaat tgatactccc ctccttgaac ctgctctcgg ccgtgatcag ggcgatgatt
     3781 tcaaggggat tcactccata tcttttggag gcatggaaga tcagccctgc ataccgtaag
     3841 gccttccctt cggaaagctg ctcattggtg atgaaccgga tcatggcggc caaattcagg
     3901 atttctttga cgttggggtc agaggtggac gctttggggg gggagaaatc ttctcggacc
     3961 tccgaaacct taaaggtctc tgtttgactg gattgggcgg aggggaaaaa atatccctgg
     4021 gatagaaaaa aagcagtggc aatgatggca accattttgc ccatttccag gatggcatga
     4081 aaagtcttcc ctcgagagat tctccggcac aag
//
LOCUS       DASPRR010000015         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1294572, whole genome shotgun sequence.
ACCESSION   DASPRR010000015 DASPRR010000000
VERSION     DASPRR010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294572"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1679)
                     /locus_tag="VEL68_00625"
     CDS             complement(135..1679)
                     /locus_tag="VEL68_00625"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ34500.1"
                     /translation="MIKTVQQYLESLNDGRQVWCLGEKVSDVRTHPTLSSIIKTACMD
                     YVLPNHPDFRELFVTRNEDGEEVNFLLASPKAAEDLLRRRECYIIGVRSGGGVIVHCM
                     GADALAAFTVAAKVMDKELGTHYTERVENYRKVLQRQDLAITGAITDVKGDRSLHPSK
                     QKQHQDYYLRIVDRRKDGIVVRGAKVHISATPCANEALCLPCRTHGEEDKDYALAFAI
                     PLNAPGIKLLAVEPVSRTYGEEGEFDYPKTSELQPTECLIVFDDVFVPWERVFMCGEW
                     QFSRHLAYAFASYHRLFGTCKMIGKLEIITGAAALVAEYNGVEKVPHVRKKLAWMAMI
                     TETVAQLGRAACLDPETEFGHDVVMPNRMAINASKYTFASNFHQMCQHLQDIAGGLST
                     TVPTFRDWNNPEIRPYIEKYLSAKDGIPTEDRLRVMRLAKDMTCNFYQIDTIHGEGSM
                     AAQEMFLYGSADWKKLKSAARRAAHVDGWQNDPVYGKLLSPREQVKMPAVDESYRSIP
                     LTAKKK"
     gene            complement(2002..3102)
                     /locus_tag="VEL68_00630"
     CDS             complement(2002..3102)
                     /locus_tag="VEL68_00630"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HXZ34501.1"
                     /translation="MDISNEKVAAAGGQPIYELVSRRDRGQAVAVMAQGAAKITRELF
                     DRGEISGVISLGGGSGTVIGTRAMRTLPLGIPKVTVSTMASGNVRLYVGISDICMIHS
                     VVDISGLNRVTKKILGNAAAAVCGMVEEEEDLSLDGRPLIAVSMFGVTTPCVTEARKI
                     LEEKGYEVLVFHANGPGGMAMEQLIAEGHIQAVLDISTTELADELRGGKCSAGPNRLE
                     KAGEMGIPQVVVPGAMDMVNYFPDAIPAQFRKRLFHVHNPATTLMRTDEEENRQLAEI
                     MAKKLSRARGPVVVYIPLRGFSAIDAPGQPFFFPEANRAFIAALRTNLPSRIPVMEKD
                     MHINDPSFARELAKALLDLLAKSHPNESVKNL"
BASE COUNT          656 a          933 c          879 g          728 t
ORIGIN      
        1 aaatttgact ctcgatagtt actggaagta atgtaccctg ctagtcttta cacatgcggt
       61 caacgaaact acctcctatg gtatataagg cataggaaca attaaatctt gcggcagccg
      121 gcggtcctct ccggctattt ctttttcgcc gttaggggta tgctccggta ggattcatcc
      181 accgcgggca ttttgacttg ctccctggga ctcagcagct ttccgtaaac ggggtcgttc
      241 tgccagccgt ccacatgggc agcccttcgg gcggcggatt tgagcttttt ccagtcggcg
      301 ctgccgtata agaacatttc ctgggcggcc atggacccct ccccgtgaat ggtgtcgatc
      361 tggtaaaaat tgcaggtcat atccttggcc agccgcatca cccgaagcct gtcttccgtg
      421 ggaattccgt ccttggcgct caggtatttt tcgatgtagg gccgaatctc cggattgttc
      481 cagtcccgga aagtcggcac ggtggttgac aatcctccgg caatatcctg aaggtgctgg
      541 cacatctggt gaaagttgct ggcaaaagtg tacttggaag cattgatggc catgcggttg
      601 ggcatgacca catcgtggcc aaattccgtc tcggggtcca ggcaggccgc ccttcccagc
      661 tgggctactg tttccgtgat catggccatc caggccagct tcttgcgcac gtgggggacc
      721 ttttctaccc cgttatactc ggcgaccaag gctgccgccc cggtgatgat ttcgagcttg
      781 ccaatcatct tgcaagtgcc gaaaaggcgg tggtagctgg cgaaggcgta ggcgagatgg
      841 cggctgaact gccattcgcc acacatgaac acccgctccc acggcacaaa cacgtcgtca
      901 aagacaatca ggcattccgt gggctgcagc tcgctcgtct tgggataatc aaattcacct
      961 tcttccccgt aggtccggga taccggctcc acggcgagaa gcttgatccc gggggcgttc
     1021 agggggatgg cgaaggccag ggcgtagtcc ttatcttctt cgccatgggt ccggcagggg
     1081 aggcagagcg cctcattggc gcagggggtg gcgctgatat gcactttggc cccccgcacc
     1141 acgatgccgt ctttccgtcg gtcgacgatc ctcaggtagt aatcctggtg ctgcttctgt
     1201 ttggaagggt gcaggctccg gtcccctttg acgtccgtta tggccccggt aatggccagg
     1261 tcctgtctct gaagcacttt cctgtaattt tccacccgct ccgtataatg ggttcccagt
     1321 tccttatcca tgaccttggc cgcaaccgta aaggcggcga gggcatctgc ccccatgcaa
     1381 tgaacgatca cccctccgcc ggatcggacg ccgatgatgt agcattctct tctccgtaaa
     1441 aggtcctccg ccgccttcgg ggaggcgagc aggaaattca cctcttcgcc atcctcgttc
     1501 cgggtcacaa acagttcccg gaaatccggg tggttgggca ggacataatc catgcaggcc
     1561 gttttgataa tgctgctgag agtgggatgg gtccgcacat cggaaacctt ttcccccagg
     1621 caccagacct gcctgccgtc attcaagctt tccaggtact gctgaactgt tttgatcatc
     1681 gagagcctcc tcctgttcgt ggcggtaaag gtgaaaccag ggtcaagtga tggtgcctgc
     1741 cggttagaaa ttctagggcg caagtcgaaa gggtgaaagt gtgaaatttc tcttgccccg
     1801 tgccctcgcg ccttccgcct cctgcggagg tcttaggctg tggagggaat atatcacgaa
     1861 accgtcgggg aaaaacaggg agaagtaccc cagttcattc ccctagatca tctcgaaatc
     1921 agatgagggc caaaaaagga gctgctatga aatgggcagt aatctcaggg gcccgaaggg
     1981 gcagggccct tgcgaggcct tctataggtt ctttaccgac tcatttggat ggctcttggc
     2041 taataaatcg agcagagctt tggccaactc ccgggcaaag gagggatcgt tgatatgcat
     2101 atccttctcc atcaccggga tacgggaggg caggtttgtt ctcagggctg cgatgaaggc
     2161 ccggttcgct tcggggaaga aaaaaggctg gcccggagca tcgatggccg aaaaacctct
     2221 taaggggatg taaacaacca ccgggcccct ggcccgggag agcttcttgg ccataatctc
     2281 ggccagctgc cggttttcct cctcgtcggt acgcatcaag gtagtagccg gattgtgaac
     2341 gtggaaaagc cgtttgcgga attgggcagg aatggcatcg gggaaatagt tgaccatatc
     2401 catggccccc ggaaccacta cttgggggat tcccatctcc ccggctttct ccagacggtt
     2461 tggaccggcg ctgcactttc ctccccgaag ctcatcagcc agttccgtcg tcgaaatgtc
     2521 cagcaccgcc tggatatgcc cctcggcgat caattgctcc atggccatcc ctccggggcc
     2581 gttggcatgg aagactagaa cctcgtatcc cttctcctct aaaatctttc tggcctcggt
     2641 cacgcaaggg gtggtgactc cgaacatgct gaccgcaatc agcggccgcc cgtccaagga
     2701 gaggtcttcc tcttcttcga ccatgccgca caccgcagcg gccgcattgc ccagaatctt
     2761 cttggtaacg cgattaagcc cagagatgtc caccaccgag tggatcatgc agatatccga
     2821 aatccccaca taaaggcgga catttccgga ggccatggtg gagaccgtga ccttgggaat
     2881 tcccaagggg agagtcctca tcgccctcgt cccgatgacc gttcccgaac cgccccccag
     2941 ggaaatgacg ccggagattt cccctctatc gaacaactca cgggtgatct tcgcagcgcc
     3001 ctgggccata accgccaccg cctgcccccg gtctctccgg ctcaccaatt cataaatcgg
     3061 ctggccaccg gcagcggcca ccttttcatt ggaaatatcc acgtaatccg gggaaggcta
     3121 aagaactcct acgtcaagag tcaggacctt ctgacctatc gccagaattt gatccttgac
     3181 gaagttcagt tcttcg
//
LOCUS       DASPRR010000016         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2262312, whole genome shotgun sequence.
ACCESSION   DASPRR010000016 DASPRR010000000
VERSION     DASPRR010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262312"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VEL68_00635"
     CDS             <1..355
                     /locus_tag="VEL68_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HXZ34502.1"
                     /translation="LVNTDMVALMADFFLRFAGVSWSFVVGTSDSSLIFSLRTKRANL
                     NAGQMARRMVKGLGTAGGHNRTGGGQIRIQDISPEKAEKMRQSIQKRFLKYAGLENVH
                     EEKLLRDISLVKEMT"
     gene            complement(421..1128)
                     /locus_tag="VEL68_00640"
     CDS             complement(421..1128)
                     /locus_tag="VEL68_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34503.1"
                     /translation="MLKVQDIETWYGSAQALKGVSLKVEKGELISILGANGAGKTTLL
                     RTISGLIEPKKGIIEFDGQRIDRLGAEEIVRLGLSHCPEGRKLFPQMTVYKNLVLGAY
                     VRKADKQGTGGTMEEVFGLFPVLKDRREQLAGTLSGGEQQMLVIGRGLMSRPKLLMLD
                     EPSLGIAPLLVARIFEVIKDISSRGMTTLLVEQNAAVALNIAHHGYVLETGEIVLSGE
                     ARQLLSEEKIKQAYLGV"
     gene            complement(1121..1882)
                     /locus_tag="VEL68_00645"
     CDS             complement(1121..1882)
                     /locus_tag="VEL68_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34504.1"
                     /translation="MLEVQGLCKDFGGLRAVDEVDFHVREGEIYSIIGPNGSGKTTIF
                     NVITGFHPATQGKIFFRGENITNLKPYTVAQKGIARTFQLTAVFSRDTVFDNLVVGHR
                     LRVKAGLLGTVFGSRMTRQEEGKCRKKAEEVLEFTGLTRDRNKIAGSLTQEAQKRLSI
                     GMALATEPRLLLLDEPTGGVNFKEISNLVSLIEKIKKGGITVCLIEHKMKVAMELPDR
                     VMVLNYGRKITEGAPGEVSQDKRVIEAYLGKSYVA"
     gene            complement(1891..>2830)
                     /locus_tag="VEL68_00650"
     CDS             complement(1891..>2830)
                     /locus_tag="VEL68_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34505.1"
                     /translation="LLVLLAFTIPFFVTSRFYLHILIMCFIWGMAATSMNLLMGYTGQ
                     VNLAHGAFFGIGAYAAGLLILKLGVNFWPALVLACIITVACGFFIGVLALRTKGSYFA
                     IGTMCFNVIVTVIIDSWEELTEGARGLLGIPRPSPIPLPLGGQITFASMPSYYYLVLI
                     SLLLTLLVIYRIVHSMMGRSFMAIRGNEELAESIGIHAMRTKLLSFLISTVFAGLAGV
                     LYASYIGFLSPEISDYHVTFEFLIFCLIGGLGTLGGPLIGAFLLTVVSELLHGVAVPR
                     LVAYGFLLVIIIIFLRGGIVGGVRMLWMRFCGGEGR"
BASE COUNT          642 a          847 c          777 g          564 t
ORIGIN      
        1 tctggtcaac accgacatgg tcgccctcat ggctgacttc ttccttcgct ttgcgggagt
       61 ctcttggagc ttcgtggtgg gcacgagcga ttccagcctc atcttctccc tgaggacgaa
      121 acgggccaac ctgaacgccg gccaaatggc ccggcgcatg gtgaaagggt tgggaacggc
      181 gggcgggcac aaccggacgg gcggggggca gattcgcatc caggacatct cccccgaaaa
      241 ggcggagaag atgcgtcaat ccattcaaaa gcgcttcttg aagtacgccg gcctggaaaa
      301 cgtccacgaa gaaaaactcc tccgcgatat ctctttggtc aaggaaatga cctaaaggaa
      361 acccagaagg atagacctcc ctctctcatc aaaatccagc gaagggatga agggacgaaa
      421 tcaaaccccc aagtacgcct gctttatttt ttcttcgctt aacaactgcc gcgcttcccc
      481 ggaaaggaca atctcccccg tctccagcac gtacccgtga tgagcgatgt tcagggcgac
      541 cgcggcgttc tgctccacca ggagggtggt catcccccgg ctgctaatgt cctttatgac
      601 ttcaaagatc cgggccacca gaaggggggc gattcccagc gacggttcgt cgagcatgag
      661 caatttcgga cgggacatga gaccccggcc gatcaccagc atctgctgct cgccgccgct
      721 cagggtcccg gccagctgct cccgccggtc cttgagcaca gggaaaaggc caaagacctc
      781 ttccatggtt ccccccgttc cctgcttgtc ggctttccgc acatacgccc cgagaacaag
      841 gtttttgtac acggtcatct gaggaaagag cttccttccc tccggacaat gggagagtcc
      901 caaccgtacg atctcctcgg cgcccaggcg gtcgatcctt tggccatcaa actcgatgat
      961 tccctttttc ggttcgatca gcccggagat cgtccgcaga agggtggttt tccccgctcc
     1021 gttggcccca aggatggaaa tgagctctcc tttttccact ttcagggaga cccctttcag
     1081 ggcctgggca cttccatacc aggtttcgat atcctggacc ttaagcaaca tagctcttcc
     1141 ccaggtaagc ttcgatgacc cttttgtcct ggctcacttc gccaggcgct ccttcggtaa
     1201 tcttccgacc gtagttcagg accatgaccc ggtccggcaa ttccatcgcc actttcattt
     1261 tgtgttcgat gaggcaaacc gtgatcccgc cctttttgat cttttcgata agtgatacca
     1321 ggttgctgat ctctttgaaa ttgaccccgc ccgtcggctc atccaggagg agaagtctgg
     1381 gttctgtggc cagggccatt ccaatggaaa gcctcttctg cgcctcctgg gtcaggctgc
     1441 cggcaatttt gttccggtcc cgggtcagcc cggtgaactc caggacctcc tccgctttct
     1501 tcctgcactt gccctcctct tggcgggtca tgcggctgcc gaataccgtc cccaggaggc
     1561 cggccttcac ccgcagccgg tggccgacca ccaggttgtc aaagaccgta tcccggctga
     1621 aaaccgcggt gagctgaaaa gtgcgggcaa tccccttctg ggcgacggta tagggcttca
     1681 ggttggtaat gttttctccg cggaaaaata tctttccctg ggtggccggg tgaaatccgg
     1741 tgatgacgtt aaagatggtg gtcttcccag agccattggg gccgatgatg ctgtaaatct
     1801 ccccttcccg gacgtggaag tcaacctcgt ctaccgccct caggcccccg aaatccttgc
     1861 agagcccctg aacttcgagc agcttccgat tcatctccct tctcctccgc aaaacctcat
     1921 ccacagcatc cggaccccgc cgacgattcc cccgcggagg aagatgataa tgattaccag
     1981 caaaaacccg taagccacca ggcggggaac cgcaaccccg tgcaggagct cgctgaccac
     2041 cgtgaggagg aaggccccga tcaagggtcc gcccagggta cccaggcccc cgatcaagca
     2101 gaagatcagg aattcaaaag tgacatggta atcggatatc tccgggctca aaaagccgat
     2161 gtaggaagca tagagaacac ccgccagccc ggcaaagacg gtgctgatca ggaaggagag
     2221 aagtttggtc ctcatggcat gaatcccgat ggattcggct agctcctcat tgccccgaat
     2281 ggccatgaag gaccggccca tcatggagtg aacgatgcgg tagatgacca ggagggtcag
     2341 gaggagagag attaaaacca ggtagtaata agagggcatg gaggcaaaag taatctgccc
     2401 tccgagagga agcgggatgg gagaaggccg gggaatcccc aggagccccc gggccccttc
     2461 ggtcaactcc tcccagctgt caatgatcac ggtgacgatc acgttgaaac acatggttcc
     2521 gatggcaaag taggagcctt tggtccgaag ggccaggacc ccgataaaga agccgcaggc
     2581 cacggtgatg atgcaggcca ggaccaaggc gggccagaag tttaccccta atttcaatat
     2641 gagcaaaccg gcggcgtagg ccccgattcc gaaaaaggcc ccgtgggcaa gattcacctg
     2701 gccagtgtaa cccataagca ggttcatgga ggtagccgcc atgccccaga taaagcacat
     2761 gatcaggata tggaggtaaa accggctggt cacgaagaag ggaatggtga aggccagaag
     2821 gaccagcagc
//
LOCUS       DASPRR010000017         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1454280, whole genome shotgun sequence.
ACCESSION   DASPRR010000017 DASPRR010000000
VERSION     DASPRR010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454280"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VEL68_00655"
     CDS             <1..1434
                     /locus_tag="VEL68_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXZ34506.1"
                     /translation="EAHSACLPVIAIGCSSKRANAGMGAFQEVDQVAILRPITKWSER
                     VTEPDRIAWAMRRAFSLSTQGRPGPVYVDIPRDTGAKEAAASRYVSVEYPLRTAGDPQ
                     KIKEAAGLLLKARNPVLVAGGGAVSSRAFEEVRKIVELLGLPLLTTPCGRGILSEEHR
                     MAFGLVGLYFSEIGEKVYGEADLLMTLGSRNEDFQSGEQRFFPKGAKYIQVDLDPEEM
                     GRNWVPDVALVGDIKLVLQALLRDLQRAGLKKGEPNPRMAKLLHAKEAYETRVKKECL
                     NDASIPLKTKRIVRELYEVFGKDTILVNENGSQDLWSYYWPYYKVGSINSCVAPGEQT
                     CMGGGCSAAIGAKLAMPEKDVVCTTGDGAFQMFMKELATAAQHKAPVTYVVLNNFSLG
                     WIKFAQRSRGNRFISTDYKVQPDFVKIAQANQCHGERVEKPEDIRPALKKALEATRKG
                     VPAVLDFIVDGWDFAPGFKRFYERLAG"
     gene            1649..3274
                     /locus_tag="VEL68_00660"
     CDS             1649..3274
                     /locus_tag="VEL68_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ34507.1"
                     /translation="MVARGSKERRKKSARGKGKLRSPRTTRMNRGDAAKKRVTLRPKA
                     GSLFPKGDYETDVLIVGYGAAGANAAIAAHDAGARALVIEKLGFPGGNSGVCAGAMVI
                     PESLEEAIQYYRALSFGTAEEEMIRSFAEEMVNIPRLLTELGIEFKVRRTEPAYFPSF
                     LTGQVKRIQVSPTGADGFRFLQNLVQDRKVEVLMDTPATALIQDPRTREVIGVKARRK
                     GKEITFLARRGVILACGGYEYNRGMIADFHFPGATDYIFPWGTPGNTGDGIKLASEAG
                     AALWHIASIEWGAFCARKPSQKFGTAVGAGLGRAMAEGSFVFVNKYGKRFMPENTSLI
                     HRKAPLEILFFDHERAEYRNLPAYMVFDETYRRRGPVASTREHFQEMWGGPVGYPTVH
                     KIYEWSQDNQAEINQGWMFQADTLAALAGKIGADASALEETVRNFNQACREGRDLQFG
                     RPGKSLAPLETPPYYAVELALTLVNTQGGPKHNKDCQVLDFGNRAIPRLYAAGEVSSF
                     FGFLYQGGNNYPEAWAFGQIAGRKAAAETPWGR"
     gene            3405..>3637
                     /locus_tag="VEL68_00665"
     CDS             3405..>3637
                     /locus_tag="VEL68_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34508.1"
                     /translation="MRGRKVLWILLILFSAGLAVPGEHFAAEKKFPTKPINVIIPFQP
                     GDTDNNLRPFTDKMVQYLGQPVNFVYKPGASGAV"
BASE COUNT          843 a          932 c         1139 g          723 t
ORIGIN      
        1 gaagcccact ccgcctgcct cccggtgatt gccatcggtt gtagctccaa gcgggccaac
       61 gcaggcatgg gggcttttca ggaggtggat caagtcgcca ttctgcggcc cattaccaag
      121 tggagcgagc gagtcacgga gccggaccgg atcgcctggg ccatgcggcg cgccttttcc
      181 ctgtccacgc agggaaggcc ggggccggtt tacgtggata tcccgcggga tacgggtgcc
      241 aaagaggcag cagcgtcccg gtatgtttcc gtggaatatc ccttgaggac cgcgggggac
      301 ccgcaaaaga tcaaggaggc agcaggtctt ttgctgaaag cgagaaaccc ggtcctggta
      361 gcgggcggag gagcagtcag ctcccgggcc tttgaggaag tgagaaaaat cgtcgagctt
      421 ttgggcctgc ccctgttgac gactccctgc gggagaggca tcctctccga agagcatcga
      481 atggctttcg gcctggtggg actgtatttt tctgagattg gagagaaggt ctatggggaa
      541 gcggaccttt tgatgaccct aggctcgcgc aatgaagact tccaatccgg agaacagaga
      601 ttctttccga aaggggctaa gtatatccag gtagacctgg acccggaaga gatgggccgg
      661 aactgggttc ccgatgttgc cctggtgggg gatatcaaat tggttttgca ggccctcctc
      721 cgcgatcttc aaagggctgg attgaaaaaa ggagagccga atcccagaat ggcaaagctg
      781 ctccatgcca aggaggccta tgaaactcga gtcaagaagg aatgcctgaa cgacgcatcc
      841 attcccctga aaaccaaacg aatcgtccgt gaactttatg aggtttttgg caaggacacc
      901 attctggtga acgaaaacgg ctcccaggac ctgtggtcct actactggcc ctattacaaa
      961 gtggggagca ttaactcctg cgtagctccc ggagagcaga cttgcatggg aggggggtgc
     1021 tctgcggcca tcggggccaa gctggccatg cccgaaaaag atgtggtatg caccaccggg
     1081 gatggggcct tccagatgtt catgaaagag ctggccaccg cggcccagca taaggcgccg
     1141 gttacctacg tggtcctgaa caatttcagc ctgggctgga tcaagtttgc tcagagaagc
     1201 cgtggaaacc ggttcatttc taccgattac aaagttcaac cggatttcgt caaaatcgcc
     1261 caggccaacc agtgccacgg agagcgggtg gaaaaaccgg aagatatccg ccctgcgttg
     1321 aaaaaagcgc tggaggcgac ccgaaaaggc gtccccgcag tcctggattt catcgtggac
     1381 gggtgggatt ttgctccagg tttcaagagg ttctatgagc ggcttgcggg gtgatgggca
     1441 gaatgagtcc ttccgtttga ccttctttta cattgccaat gcaaaatcgt tattccgcat
     1501 gggaaatgct aagggtaaac ggatggcgga gaatcaatct gagacataaa cgccgctccg
     1561 aaatttttct tgtgatgagg aaatgaaaga tgtatgttcg caatgaaata acccgaccaa
     1621 gtcacataga ttattaaagg agggaagaat ggttgcgagg ggaagcaaag aaaggcggaa
     1681 gaagtccgct cgggggaaag gaaagctgcg gagtccccgc acgacgcgaa tgaacagggg
     1741 cgatgcggcc aagaaacggg taaccctgcg accgaaggcc ggctccctat ttccgaaggg
     1801 ggattatgag acggatgtgt tgatcgtcgg gtatggcgcg gccggggcga atgcggccat
     1861 cgccgcccac gatgccggag cgcgggcact ggtcatcgaa aaattggggt tccccggagg
     1921 aaacagtggg gtttgcgccg gggccatggt gattccagag agcctcgagg aggccatcca
     1981 atattaccgg gccctctcct tcggcacggc ggaggaagag atgatccgtt cctttgccga
     2041 ggagatggtc aatatccccc gtctactgac tgaattggga atagaattca aggtccggcg
     2101 gacggaaccc gcctatttcc cctcttttct gactggccag gttaaacgaa tccaggtcag
     2161 ccctaccggg gcggacggat ttcgttttct ccaaaacctg gtacaggatc ggaaggtcga
     2221 agtccttatg gataccccgg ccacggccct gatccaggac cctcggaccc gggaagtcat
     2281 aggggtgaag gcgcgaagga aggggaagga aataaccttc ctggcacgca ggggggtcat
     2341 ccttgcatgc ggtgggtacg aatacaaccg ggggatgata gcggattttc actttcccgg
     2401 agcgacggat tacatcttcc cctggggcac ccccggcaat acgggggacg gtattaaact
     2461 ggcctccgag gccggagcgg cactctggca cattgcctcc atagaatggg gcgctttctg
     2521 tgcaaggaaa ccgagccaga aattcgggac ggcggtcggg gccggcctgg ggagggcgat
     2581 ggctgagggg agcttcgttt tcgtgaataa atacgggaaa aggttcatgc cggagaacac
     2641 gagccttatc caccgcaaag cgcccctgga gattcttttc tttgatcacg agcgcgcgga
     2701 gtatcggaac ctccctgctt acatggtctt tgacgagacg taccggcgca ggggtccggt
     2761 cgccagcacg cgggaacatt ttcaagagat gtggggcggc ccggttggct accccacggt
     2821 ccataaaatt tacgaatgga gccaggacaa tcaagcggag attaatcagg ggtggatgtt
     2881 tcaggccgac actctggcgg ccctggccgg gaagatcggg gccgatgctt cagcgctgga
     2941 ggagacggtg cgcaatttca accaggcctg cagggagggg cgcgatcttc agttcggaag
     3001 gccgggaaaa tcgttggctc cgctggagac gcccccctat tatgccgtcg aactggctct
     3061 cacgctggta aacacccagg gcggccccaa gcacaataaa gactgccagg tgttggattt
     3121 cggcaaccgg gcgattccgc gattgtatgc agcgggagaa gtgagttcct tcttcggttt
     3181 cctctatcag gggggaaaca attacccgga ggcctgggcc ttcggccaga ttgccgggag
     3241 aaaggcggcg gcggagacgc cttggggcag gtagcacagc gagcgatagg gatttgcctg
     3301 ctgtccagga agattttagc tttggggcca ttaacacctt gacccccccc atcatccgct
     3361 atatcatcac acaaaaagct tagattccat caggaggtgc gatcatgcgc ggaaggaagg
     3421 ttttatggat tctgttgata ttgttcagcg caggcctcgc ggttccgggg gaacattttg
     3481 cggctgaaaa gaaattcccc accaagccga tcaacgtcat cattcccttc caacccgggg
     3541 acaccgacaa taacctgcgc ccctttaccg acaagatggt ccaatacctg gggcagccgg
     3601 tgaattttgt atataaaccg ggggcgtcgg gagcggt
//
LOCUS       DASPRR010000018         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_651531, whole genome shotgun sequence.
ACCESSION   DASPRR010000018 DASPRR010000000
VERSION     DASPRR010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651531"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1341)
                     /gene="thiC"
                     /locus_tag="VEL68_00670"
     CDS             complement(61..1341)
                     /gene="thiC"
                     /locus_tag="VEL68_00670"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXZ34509.1"
                     /translation="MTQLEFARQGRITEAMEKCAAAEDVPAENIRKGLAEGSMVITQN
                     VSHRHISPLAIGKGLRAKVNANVGTSVDHNNIEEELEKVRVAIEAGADTIMDLSTGPG
                     IDETRKAVMKASTVPVGTVPIYQAAIEMPRKKNRSMVEMTADDLFEVIERHGEDGVDF
                     ITVHCGVTRNSVEKIKKEGRLLGVVSRGGAILVEWMDHNKKENPLYEDYPRLLEIAKA
                     YDMTLSLGDGLRPGCLADATDRGQVQELILLGELTENAWAKGVQVMIEGPGHVPLDQI
                     EANVLLQKRICHGAPFYVLGPLVTDIAPGYDHITGAIGGAIAARAGADFLCYVTPSEH
                     LRLPTVEDVREGVIASKIAGHAADIARGLKGAMEKDCQMARARRSLDWKEQIRLSIDP
                     RRAQKLRESSMPKESDVCTMCGDLCAIKKVAKLL"
     regulatory      complement(1462..1567)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1733..>2900)
                     /locus_tag="VEL68_00675"
     CDS             complement(1733..>2900)
                     /locus_tag="VEL68_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXZ34510.1"
                     /translation="MLSALCRSYFMSLTKMVKEKALELGFVAVGVTTPEPFTLYEEEI
                     ASRPEMYAWGRGLSQRASVRDLDLTRFVDPVRFLPEVKSLIVVTDSYFEEDFPPSLAG
                     KIGRCYLKGLFCPEDTSHSGRRREFKQFLQDLGIKSIYGPAPARLAGARAGVTNYGKN
                     CFAFANEAAGKSSWVVNEPYLVDRELEPDSPTMKVGCPENCRKCMEACPTGALYAPLK
                     MDPRNCIAYNTYFTSAEIPRDMRSKMRTWVYGCDLCQEACPRNKKWLEGKKPMSPKLL
                     ERSADFSLIQLLAMSQDDYEQKVWPLLYYIRKENRKLWQRNAAIALGNQGDPETAPVL
                     ISALGDPESLVRAHVAWALGKIGGKQAKAALERKKHLEEDEGVRREIEEALNQG"
BASE COUNT          595 a          870 c          757 g          678 t
ORIGIN      
        1 tattaaggtg atctctgaaa tccctcctga cctccctttc ataaagggag gaattattca
       61 tcagagaagt ttggccactt ttttgatggc gcaaaggtcc ccgcacatgg tgcagacatc
      121 gctctccttg ggcatgctgg actcccggag tttctgggcc ctcctcggat cgatggagag
      181 gcggatctgt tctttccagt ccagggatct tctggcccgg gccatctggc agtccttttc
      241 catggccccc ttcaaccctc gcgcgatgtc cgccgcatgg ccagcaattt tggaggcaat
      301 gactccttcc cttacatctt cgacagtggg aaggcgaaga tgctccgaag gggtgacata
      361 acaaaggaaa tccgcgccgg ccctggcggc aatggctcct ccgatcgctc cggttatatg
      421 atcgtatcca ggcgcaatgt cggtcaccag gggacccaag acataaaaag gagccccatg
      481 acaaatccgt ttctgcagaa gaacattggc ttcaatctgg tccaggggta catgtccggg
      541 tccctcgatc atgacctgga ctcccttggc ccaggcgttt tccgtcaatt ctcccagaag
      601 aatgagctcc tggacctggc cccggtccgt ggcatcggcc aggcaccccg gacgaagacc
      661 atcccccagg ctcagggtca tatcataggc cttggcgatt tccagcagcc gggggtaatc
      721 ctcgtaaaga gggttttcct tcttgttgtg atccatccat tctaccaaga tggctccccc
      781 ccggctgacg acgcccaata ggcggccttc cttcttgatc ttttccaccg agttccgggt
      841 gaccccgcaa tggacggtta tgaaatccac cccatcttct ccatgcctct cgattacctc
      901 aaagagatcg tcggcggtca tctctaccat ggaacggttc ttcttgcggg gcatttcaat
      961 ggccgcctga tagatcggta cggttcctac gggaacggtg gaggccttca tcaccgcctt
     1021 gcgcgtctca tcgatccccg gacccgtgga caggtccatg atcgtgtccg cgcccgcttc
     1081 gatggccacc cgcactttct ccaattcttc ctcgatgttg ttgtggtcca cggaggtgcc
     1141 cacgttggcg ttcaccttgg cgcggagacc tttgccgatg gccagggggg agatgtggcg
     1201 atggctcaca ttctgggtga tgaccatgga cccttcggcc agccccttgc ggatgttctc
     1261 agctgggaca tcctccgcgg cggcgcactt ctccatggct tccgtaatcc ggccctgccg
     1321 ggcgaattca agttgggtca taattcctcc ctatagaacc aacgaaccaa aaaggtcatg
     1381 gcaacgccgg aagacctttg gttctgatgc cggtgaaaac aaaaaaagcc gacgatccct
     1441 gaaagaaccg gcggctttga acccttaagg gatttgcttc cctccgctgg cattatccaa
     1501 caggttcgag gggtcatccc gccttaagcg ggatctctca gccgatccag ctcccccagc
     1561 aaatctaata ttattttaca atacttggac ggaatgtcaa ggatcaaaaa gcttagcaca
     1621 gagtgcctag agcatagctt aaaaaggaac aaactcctgc ccgatggtca accagcagct
     1681 ttcaggtgcg agccatcagc catgagtctt ccttaaacct ataccctctg ctctatccct
     1741 ggttcagggc ctcctcaatc tctctcctga cgccttcatc ttcttccagg tgcttttttc
     1801 tctccagcgc ggctttggcc tgctttcctc caatttttcc cagagcccag gcaacgtggg
     1861 cccggacgag ggattcaggg tcccccaagg cggagattag aaccggggcc gtctccgggt
     1921 ccccctggtt tcccagggcg atggccgcat ttctctgcca gagtttgcgg ttttccttgc
     1981 ggatgtaata gagaagaggc cataccttct gttcatagtc gtcctgggac atggccagaa
     2041 gctgaatcag ggagaagtct gccgatctct cgagaagttt tgggctcatg ggcttttttc
     2101 cctccagcca tttcttgttc cgtgggcagg cctcctggca gaggtcgcat ccataaaccc
     2161 aagttctcat tttcgaacgc atatcccggg gaatttctgc ggaagtaaaa tacgtattat
     2221 aggcgatgca gttgcggggg tccatcttca aaggggcata gagggctccg gtgggacagg
     2281 cttccatgca cttgcggcaa ttttccgggc agcccacctt catcgtgggg gagtcaggct
     2341 ccagctcccg gtccaccagg tagggctcat tgaccaccca ggaactcttc ccggccgcct
     2401 cattggcgaa ggcaaagcaa ttcttcccgt aattcgttac tccggcccgg gctcccgcca
     2461 gtcgagccgg cgccgggcca tagatggact tgattcccaa atcctgcagg aattgcttga
     2521 attccctcct ccgcccgctg tgagaggtat cctccgggca gaagagtcct ttcaggtaac
     2581 aacggccgat tttgccggcc agggagggag ggaaatcttc ttcgaaatag ctgtccgtca
     2641 ccacaatcag ggatttgact tcagggagaa accggaccgg gtccacgaac cgggtcaggt
     2701 ccaggtctcg gaccgaggcg cgctggctca gtcctcggcc ccaagcgtac atctccggcc
     2761 ggctggcaat ttcttcctca tagagagtaa atggttcggg cgtagtcacg ccgaccgcta
     2821 cgaaacccaa ctcgagggct ttttccttca ccatcttggt caaagacata aaataactcc
     2881 ggcatagagc agagagcata
//
LOCUS       DASPRR010000019         5482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1940880, whole genome shotgun sequence.
ACCESSION   DASPRR010000019 DASPRR010000000
VERSION     DASPRR010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940880"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VEL68_00680"
     CDS             complement(<1..390)
                     /locus_tag="VEL68_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HXZ34511.1"
                     /translation="MKRAVRHFVFNPLSLLGVLFIVLLILTGVLAPVLAPYDPIEISP
                     DRRLAPPNSANWLGTDEVGRDILSRVIHGARISLEIGVTIVFFAAVIGLVIGLISGFF
                     GGVLDQTLMRFTDMFMSFPTLILAIAMT"
     gene            complement(397..1401)
                     /locus_tag="VEL68_00685"
     CDS             complement(397..1401)
                     /locus_tag="VEL68_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ34512.1"
                     /translation="MPAFLLKRILLLIPSLIGVTVMVFVISHVVPADPVGIALGPEAT
                     QEQINAMREAWGLNQPLYVQYGIFLRNLVQGNLGYSLVSKRAVTEELGYYFPATFELT
                     TASMILSILLGIFIGIVSAVYRNRLFDHLTRTYCVFGISMPVFWLGLLLLLLFYVKLG
                     WLPGGGRIADGIQVERISGFLILDSLLTGNWEALGSALLHLVLPAFCLSSSFLATISR
                     IARSSVLQVIREDYVRTARAKGVPEFLVMSKHVLRNSILPVITISGILYGQLLGGAVL
                     TETIFSWPGMGRYVVRSILHLDFEPILGFTVLVAILYALANLMVDLLYSVVNPQIRLE
                     "
     gene            complement(1468..3066)
                     /locus_tag="VEL68_00690"
     CDS             complement(1468..3066)
                     /locus_tag="VEL68_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34513.1"
                     /translation="MKLALPEWFKGVILPLAFGLILFGPGWSAAKTPDNELVVGSPSA
                     IETMDPAQHMSIGSFKGENFVFNNIVGYAKESAKVVPELAESWTMSPDGKVITFKLRR
                     GVKFQDGTPFNAAAVKFNWDRMLKANLSPAGKYKTYADENSVEVVDDYTVRFKQTDPT
                     PVVLDYFAGGAKFYFNSPTYVQKYMKSDDSLALKWMATHACGTGPYELAEWEPDSKYV
                     FKKFAGYWGGTPEVKPAPKFERITYKIVKDPSALRMMLEKGDIDVAEKLPADAIDQLS
                     KTPGVKVKMADIWKMVFLIMNCQKAPFDNVKVRQAVSYAINYDELIKHVEKGRAKRLS
                     GTMMEGFPGYDPNLFKYNLDLDKAKQLMKEAGLENGFKTTLLYSADRYMGFELMSVMI
                     QSYLKKIGIDASLQKMAWPTQVETMKKGAFEMALQTWTANYPDPVEQTFFFFHPETFG
                     LRWNWSYWKNDRATTLIDGLMKEFDNGKRVAMIKEIQKLGVDNAVYGYFYQVPHAIAM
                     RDNVEDVWFHPGVDWIPSPAYKKK"
     gene            3422..4387
                     /locus_tag="VEL68_00695"
     CDS             3422..4387
                     /locus_tag="VEL68_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34514.1"
                     /translation="MKRSWIIGTVALLFLFSGSAFSAEPIKLAMSTWLGYAPLYLAKE
                     KGFFQKRGIEVEVVVIESPADRRVAFAADKIQGFCTTVDTHVMTAAAENPIPLKQVLA
                     LDDSHGGDGMVSKKEIKTIKDLKGKTVAAQLGAGASYFWLNYVLSQNGMKLSDLKTID
                     MKAGDAGSAFVAGKVDAAVTWEPWLSKAKETPFGHVLLSSDKTPGIIVDSLAFKPDFL
                     KKRGEDVKKIIAAWNEAVKFAADSPQEADAIMAKFTGQKPEEFTKEKTGVRFYGEKEN
                     KEYFGTAKNPGLLYKVTQRAADLWSELKLIKKKPKAADLIDGSFL"
     gene            4405..5235
                     /locus_tag="VEL68_00700"
     CDS             4405..5235
                     /locus_tag="VEL68_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ34515.1"
                     /translation="MSAGRSGKPTERAPRRLKDFFHPREPIPNSLYVFLTFGSFGFFL
                     VAWAILTYGGVIDPLFLPSPGRVFEAGYDLFSELSFSTDILNSVYRVMAGFLLAALIG
                     VPLGLIMGTFKAAEAFTEPVVGFIRYMPASAFIPLFILWLGIGDVEKVAIIFVGSFFQ
                     LVLMVAVVAKNVHKDMLETAYTLGAKRFQVIRKVLLPASMPGIVDTLRIIVGWAWTYI
                     IVAELVASASGIGYMIISSQRMLRTANIIFGILTIGMLGLITDYFFKWLYRRLFPWMD
                     "
     gene            5240..>5482
                     /locus_tag="VEL68_00705"
     CDS             5240..>5482
                     /locus_tag="VEL68_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ34516.1"
                     /translation="MGEEVKLHIHNVTKVYCSGEQEVLAIDGVDLQIRNKEFATILGP
                     SGCGKSTLLRIVAGLTKPTSGVARMDGKVITEPGQDR"
BASE COUNT         1291 a         1473 c         1523 g         1195 t
ORIGIN      
        1 ggtcatggcg atggccagaa tcaacgtggg gaacgacatg aacatatccg tgaagcgcat
       61 gagagtctga tccaggactc ccccgaaaaa tccactgatc aggccgatca ccaacccaat
      121 gaccgcggcg aagaacacaa tcgttactcc gatctcaaga gagatgcggg ccccgtgaat
      181 gacccggctc aggatgtcgc ggcccacttc gtcggttccc agccagttgg ccgaattggg
      241 aggcgccagg cgtcggtccg gagaaatttc tattggatca taaggagcca aaacaggagc
      301 caaaacccca gttaaaatga ggaggacaat aaacaggacc cctagaagag atagaggatt
      361 aaagacaaaa tgacggaccg ctcgcttcat aaggtgtcat tccaatcgga tctggggatt
      421 caccactgag tacaagagat caaccatcag attggccaga gcataaagga ttgccaccag
      481 gaccgtgaag cccaggatgg gctcaaaatc aaggtgcaag atggagcgca ctacatagcg
      541 acccatcccc ggccaggaaa aaatggtttc ggtcaagacc gcccctccca ggagctgacc
      601 gtagaggatc ccggaaatgg ttatcaccgg cagaatggaa ttacggagaa catgtttgga
      661 catgaccagg aattcaggga cccccttggc ccgggcggtc cggacataat cttcccggat
      721 cacctgcagg accgaggagc gggcaatgcg ggaaatggtt gccaggaagg aactggagag
      781 gcagaaggcc ggcaaaacca ggtggagcag cgcggaaccc agagcctccc agtttccggt
      841 caagagacta tccaggatca aaaagccgga gatacgctct acctggatcc cgtcggcaat
      901 tcggccgccg cccggaagcc agcccagctt gacatagaag agaaggagaa gaaggagacc
      961 cagccagaag accggcatgg agataccgaa cacacagtag gtccgggtga gatgatcgaa
     1021 gaggcgattc cgatagaccg cggaaacgat gccgataaaa attcccagga ggatggaaag
     1081 aatcatgctg gcggtggtca gctcgaaggt agcgggaaaa tagtaaccga gttcctcagt
     1141 cacggcccgc ttgctgacca gcgaataccc caggttcccc tggaccaaat ttctcaggaa
     1201 gattccgtac tgaacataga gaggctgatt caggccccag gcttcccgca tggcattgat
     1261 ctgctcctgg gtggcctccg gaccgagggc gattcccacg ggatcggcgg ggaccacatg
     1321 ggagatcaca aagaccatga cagtcacccc gatcaggcta gggatcaaga gcaggattcg
     1381 ttttaggagg aaagcaggca cgggaagccc ttaaaaaaag agggttgaac cccctaaaga
     1441 gattcaaccc tcttcttcgg ctggggtcta cttcttttta tatgcaggag aagggatcca
     1501 atcgaccccc gggtggaacc acacatcctc cacattgtcc cgcatggcga tggcgtgggg
     1561 aacctggtag aaatagccgt acaccgcgtt gtccactccc agcttctgaa tctccttgat
     1621 catggcgact cgtttgccgt tatcgaattc cttcatgagg ccgtcgatga gcgtcgtggc
     1681 ccggtcgttc ttccagtaag accagttcca gcgcaggccg aaggtctcgg ggtggaagaa
     1741 gaagaaggtc tgctccactg ggtcgggata attggccgtc caggtctgga gagccatttc
     1801 aaaagccccc ttcttcatgg tttccacctg ggtcggccag gccatcttct gcaggctcgc
     1861 atcgatcccg attttcttca ggtacgactg gatcatgacg ctcatcaatt caaatcccat
     1921 gtagcggtcg gccgaataga gaagggtggt cttgaacccg ttctccaacc cggcctcttt
     1981 catcaactgc ttggccttat ccaggtccag gttgtatttg aagaggttgg gatcgtaccc
     2041 cgggaatccc tccatcatgg tgccggagag tctcttggct cttccctttt ccacgtgttt
     2101 gatcaattca tcgtagttga tggcgtaaga tacggcctga cgcaccttca cgttgtcaaa
     2161 gggcgctttc tggcagttca tgatcaggaa gaccatcttc cagatatcgg ccattttaac
     2221 tttgaccccg ggggtcttgg aaagctgatc gatggcatcc gcgggcagtt tctcggccac
     2281 atcgatgtct cccttttcca gcatcatccg caaggccgaa ggatctttta cgatcttata
     2341 ggtgatcctt tcaaacttgg gcgcgggctt gacctcgggt gtccctcccc agtacccggc
     2401 gaacttttta aagacatact tggaatccgg ctcccattcg gccagctcgt aagggcccgt
     2461 cccgcaggca tgggttgcca tccatttaag ggccagggaa tcatctgatt tcatgtattt
     2521 ttgaacgtat gtagggctgt tgaaatagaa cttggcaccc ccggcgaaat aatccaatac
     2581 cacgggggtc gggtcggttt gcttgaagcg aacggtatag tcgtccacga cttctacact
     2641 gttctcatcc gcgtaggttt tgtacttccc ggcgggagat aggttcgcct tcagcatccg
     2701 gtcccagttg aatttcaccg cggcggcgtt gaaaggggtc ccgtcctgga atttgactcc
     2761 ccggcgcagt ttgaaggtga tgactttgcc gtcgggcgac atggtccagg actcggccag
     2821 ctcaggaacc accttggccg attctttggc atagcccacg atgttgttga atacgaagtt
     2881 ttcccctttg aaagaaccga tggacatgtg ctgggccggg tccatggttt caatggcgct
     2941 gggggaaccg acgaccaact cgttgtccgg cgtcttggcc gcgctccatc ccgggccaaa
     3001 aaggattaag ccgaaagcca gaggcagaat cacccccttg aaccattcgg gcaatgccaa
     3061 tttcattttt tcctccttct ttgctccctc aggattccag ggatttccac cgccgagtat
     3121 aaaagcattc tcgggttgat gctcataccc atagttccgg agggaatcaa ttcctgggaa
     3181 aaatgagatt cgctttaaga ccatttaata gctcaattgc ctcccgttgt caaattattt
     3241 cgggatattc ttgaccaatt ccatcctaga atgtccggcc ggaatttcgt ccctcttcgg
     3301 actatcgggc caagaggtat gcttacaaat ccattgattt tcttttccct ttatgaaaaa
     3361 atccaccagt gagatagggc cttagaccgg aaaaaataaa attaagaagg gagatggagg
     3421 aatgaaaagg agttggatca tcggaaccgt agctttatta tttttgttct ccggctctgc
     3481 cttttcagct gagccgatca agttggcgat gtccacctgg ctagggtatg cccccctgta
     3541 cctggccaaa gagaagggct ttttccagaa gcgggggatt gaagtggagg tagtggtcat
     3601 cgagagcccg gctgaccgaa gggtggcctt cgccgctgat aagatccagg ggttttgcac
     3661 caccgtggat acccatgtca tgaccgccgc ggcggagaat cccatcccct tgaaacaggt
     3721 cctggccctg gatgattctc acgggggaga cgggatggtc tccaagaagg agatcaagac
     3781 catcaaagac ctgaaaggaa agaccgtggc tgcccagctg ggtgccggcg cgagctattt
     3841 ctggctgaac tatgtcctct cccagaacgg aatgaagctc agcgacctca agacgatcga
     3901 tatgaaggcc ggggatgcgg ggtcggcctt cgtcgccggg aaagtggatg cggcggtaac
     3961 ctgggagccg tggctttcca aagcgaagga gacccctttt ggccatgtcc ttttgtccag
     4021 cgacaagacc ccggggatca tcgtggactc tctggccttc aaacccgatt tcctaaagaa
     4081 gcggggagag gatgtaaaga aaattatcgc tgcctggaac gaagcggtta agtttgcggc
     4141 cgatagcccc caggaagccg atgccatcat ggccaagttc accggacaga aacccgagga
     4201 gttcaccaag gagaagaccg gagtccggtt ttatggagag aaggagaaca aagaatattt
     4261 tgggaccgca aagaacccgg ggctcctcta caaagtgacc cagcgggccg ccgacctctg
     4321 gtcggaattg aagctgatta aaaagaaacc caaggctgcc gacctcatcg acgggtcgtt
     4381 cctataggca tcgccgggtt ttggatgtcg gccggaagat caggaaaacc aactgaaagg
     4441 gctccccgga ggctcaagga cttcttccat ccgcgggagc ccatcccgaa tagtctttat
     4501 gtctttttga ccttcggcag ctttggcttc ttcctggtcg cctgggcgat tctgacttac
     4561 gggggggtta tcgatcccct tttccttcct tccccgggaa gagtcttcga ggcgggctac
     4621 gacctcttct cggaactgag cttcagcacc gatatcctca acagcgtata ccgggtaatg
     4681 gccggctttt tgctggctgc cttgatcggg gtccccctgg ggctgatcat ggggactttc
     4741 aaggctgctg aggcattcac cgagccggtc gtcggcttca tccgctacat gccggcttct
     4801 gcattcatcc ccctcttcat cctgtggctg ggaatcgggg atgtggaaaa ggtcgccatc
     4861 atcttcgtgg gtagcttttt ccagctagtg ctcatggtgg ccgtggtggc caagaatgtg
     4921 cataaagaca tgctggaaac cgcctacacc ctcggcgcca agcgtttcca agtcatccgc
     4981 aaagtgctcc ttccggccag catgccgggg atcgtggaca ccctgagaat tatcgtcggc
     5041 tgggcctgga cctatatcat cgtggccgag ctcgtcgcct cggcttccgg gatcggatac
     5101 atgatcatca gctcccagag aatgctccgg acggccaata tcatctttgg cattctgacc
     5161 atcgggatgc tggggctgat cacggattat tttttcaaat ggctatatcg tcgtttattc
     5221 ccctggatgg attaggaaag tgggcgaaga agtaaaactt cacatccaca acgtgaccaa
     5281 ggtctactgc tccggcgagc aggaggtact tgccatcgac ggggtggacc tgcagatccg
     5341 caataaggaa ttcgccacca tcctgggccc ttcgggatgc gggaaatcca cccttctccg
     5401 gatcgtggcc ggattgacca agcccacctc aggggtagcc cggatggatg gcaaggtcat
     5461 caccgagccc ggccaggacc ga
//
LOCUS       DASPRR010000020        10671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1297840, whole genome shotgun sequence.
ACCESSION   DASPRR010000020 DASPRR010000000
VERSION     DASPRR010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10671)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10671
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297840"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1100)
                     /locus_tag="VEL68_00710"
     CDS             complement(306..1100)
                     /locus_tag="VEL68_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34517.1"
                     /translation="MLRLNNIEVIYSDVILVLKGVSLEVAPGKIVALLGANGAGKTTT
                     LKAVSGVLHTEEGEVTDGSIEFEGQRIDRLDPEEIVRLGIVQVMEGHRLFEHLTVEEN
                     LFVGAYARGDGPAVKRDLQRVYEYFPRLMELSPRTSGYLSGGERQMLVMGRGLMAHPK
                     VMLLDEPSLGLSPVLVREIFRIARRINQEEKASILLVEQNAVAALSVAEYGYVMENGK
                     VVLDGPTSKLKDNEDIKEFYLGLSGVGKRKSYREVKHYKRRKRWLG"
     gene            complement(1120..1629)
                     /locus_tag="VEL68_00715"
     CDS             complement(1120..1629)
                     /locus_tag="VEL68_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34518.1"
                     /translation="MKRFSWFIYLVMIVSLLGACKSTTPPPEQLATSAPAPEPPPPKV
                     TLPTTLPAMLSILQTRIYFSGSGYQPKEMVVVEMEVPPGVEIPAVKPGEPVGVAFGYA
                     DEKGDFTADITPPTKIMTFLRGDISPTLAPDPKSFKPIPHGVYTFRITGAESGRTALS
                     KIEFVPPGK"
     gene            complement(1814..3037)
                     /locus_tag="VEL68_00720"
     CDS             complement(1814..3037)
                     /locus_tag="VEL68_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34519.1"
                     /translation="MRKAGGGFIFVILALCILLCTLEGDSWAQEKMVTYLSLADYTGA
                     IAGLNVPADMGVEDYFKDLNARGGVDGVKVKFIGVDTRYDVARGVSAYKRYRTEHKLL
                     VVNAIGTPIGKAVAPLATKDRLVQLVPGDGEFQAKLGRIFIWGPAYQDAFSAAMDWVV
                     MDWKAKGKPGMPKFGFISWDSPYGKEFLRGGNEYADKIGLPMVKPEFFPPGALDHTVY
                     LTRLAGAGANYIFAGGVDPTPTNIIRDAHKLGLTKTIQFLNDYWGPTEPVGIRAHPEA
                     LEGTVITSYYLRGQDAKNHPGSSLWTKYRGKPITDFNETYLAGMVWGMDFEEGLKIAL
                     KDVGYEKLDGEAMFKAYQKLAGFKRQGITGINAYSPTSRRGSLEVKFYQVKNSRAVAI
                     TDWIKAPDAVSLHKF"
     gene            complement(3104..4150)
                     /locus_tag="VEL68_00725"
     CDS             complement(3104..4150)
                     /locus_tag="VEL68_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34520.1"
                     /translation="MLPCGVYNERYAQDMAIVRTKRHWAILLISLFLLYLFPAFGQEY
                     FISLINYFCITFIIVLGLQVVTGFCGQVSFGQAAFMAIGAYTSAILTLKFGVTFWLAL
                     PLSGISAGVIGLIGGAPSLRIKGFYLAMATLAIHFLIMWLIMHLEITGSTQGLHPAPP
                     SIGEFQFDSDGRMYYIIVSVMLLMTYGAKNMMRTRLGRAFVAIRDNDLAAEVMGINLY
                     RYKLLAFFISCFYAGVAGSLWAHWIQVVHPEQFTVLHAVWYVGMLIVGGMSLTAGAFF
                     GVIFLRGLDELVMFSSPFLASTFPWLGTHPAASLGITAFGLILMVFLIFEPRGIAHRW
                     EIFKTSYRLYPFAY"
     gene            complement(4152..5036)
                     /locus_tag="VEL68_00730"
     CDS             complement(4152..5036)
                     /locus_tag="VEL68_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34521.1"
                     /translation="MTEFLQLAITGLLTGGLYAMVGAAIVLVYKSTHVVSLAHGQFLA
                     FGAFFFYVFIVVLALPFWAAILLTFLVAGAIGLAAERFSLRPLIGQPLFSAFLMTFAI
                     FMTMDGIFQLILKGETRTLPRFLNTETLSVGGLNIPTGGILSFGVALGLFFLLALLFR
                     FTKIGLGMRATAEDHQLAQSTGISVRTIFSIVWIVSALVAAVAGISLARVMDIHYPLP
                     WVGLKGLVVALFGGLDSLPGALLAGLILGMLENLSAGYLDPLVGGGVGDVAAYVMLLL
                     ILLVKPFGLFGLKRIERI"
     gene            complement(5346..7268)
                     /locus_tag="VEL68_00735"
     CDS             complement(5346..7268)
                     /locus_tag="VEL68_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ34522.1"
                     /translation="MVSQEETPRNLDTLPKLLRWNYRKHGDRKMAMRVKDRGIWQSYT
                     WKDYYEKVKYLSLGLMKLGLERGDKVSILGENKPEWYWAELAAHCAGATVVGIFTDCI
                     PSEVKYYLEHSDSKFVVAHDQEQVDKLLEIKGQLPLLKKVIFWDPKGLWHYHDPLLFS
                     FEEVLKLGAAQEKVNPQLFEQKIDQGQSSDIGVICYTSGTTGLPKGAMLSQRWLVDGV
                     RAWALMDDWNEKGYQYLSFIPPAWATEQGLGIGGSLVADVSVNFPEEPETVQENLREI
                     GPQILFYGARLWENVNRMVQARMIDSTALRRLLYRLCLPLGYRMADILGAGKTPSWLD
                     RVLYVLAYQTVFRALKDKLGLSNARCVYSAGAAVSPEIIRFFQALDVQIKLFYGSTEL
                     GVVSVPRGRGIRPETSGTVVPWAEVRLSDEGEILVKSQFMYSGYYKDPEATARKLKDG
                     WYATGDFGHIDENGHLIVIDRMEDLKALAGGKKFSPQYTEVRLRFSPYIKDVLVVGGE
                     DREFVTALINIDLENVGRYAESKRIPYTTFTDLSQKPEIVELVRKELEKVNRTLPDYA
                     RVKRFVNLHKEFDADEAELTRTRKLRRTFVEDRYKDLVMALYGDSPEYAVEAAITYRD
                     GRKGVIKTAIHINKIN"
     gene            complement(7261..8064)
                     /locus_tag="VEL68_00740"
     CDS             complement(7261..8064)
                     /locus_tag="VEL68_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34523.1"
                     /translation="MPASHPTGGGDQIVLRVDDLDLRFGGMMALSGVSLEVRDAEILA
                     IIGPNGAGKTCTLNCINGFYRPQRGEVFFQGERITPLSPHRIAKLGISRTFQNIELFT
                     GLNTLDNLMAARHIHMRRGALAGFLYFGPMRREEIAHRKVVEDIIDFLELEPVRKKTV
                     GLLPYGLRKRVELGRALALEPKILLLDEPMAGMNVEEKEDMARFIIDISELKRVPIAL
                     VEHDMGVVMDIAQRVIVLDFGQKIAEGTPADIKQDPRVIKAYLGEGEIG"
     gene            8438..9220
                     /locus_tag="VEL68_00745"
     CDS             8438..9220
                     /locus_tag="VEL68_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HXZ34524.1"
                     /translation="MRWERTPGGTLIAMAFLFTLLFLGCARTRQPVSEPVLPKPETSE
                     VVSEVQKTDRVMVPSASAVPTPPTPQPEPPIPEPPIPEPQAAPKPNSGAEEAAQDRIA
                     SGAVLLNPSVFEDAKAIQSRLAELGVYKMAIDGMWGRDSRAALRFFKEENNLKFPDQW
                     DKETQVALFGKGGAPGPPVDTSDPLASGTLLLTPSNPEDVETIQGRLAELGFYSGGID
                     GIWGRDTRAALRAFKEKIGLKNPEQWDKETQMLLFRELGKWP"
     gene            9563..>10671
                     /locus_tag="VEL68_00750"
     CDS             9563..>10671
                     /locus_tag="VEL68_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ34525.1"
                     /translation="MRIREIFSLISFLVLAFSTLPLAEVMAQPKEIVLGTALPLTGGQ
                     SREGGYFKKGYELAVKEINDGGGIPIKEYGKNIPIKLIIYDDKSDNTTSVQLYEKLVT
                     QDKVNALLGGYGTPLITAHTIVAEKYRVPYLNGGGATAAIYQRNMKYVFGILASIEKL
                     AHTLMDWVSAQQDAGHLKKPLKISVMGENTSHGKEFRQGVLDKSKAAPARFQIVMDEP
                     FELNLKDADPLLQKLKASDADVFLADARLADYTTIHRRYTELRLYHQIVSYGPRGSEK
                     AARDALGEASDYIVSCNWWHKEVPTPAARKFAEKYAQTYHEPAEWFPALAYETVRVMA
                     KAMEMAGSLDKSKIRDALAKMDMKDSLVVGGRVWF"
BASE COUNT         2355 a         3087 c         2883 g         2346 t
ORIGIN      
        1 caatttctcc ttcattcctg catctccttg aattctggct ttgttcgaaa gtgggtgatt
       61 cgtcgaaggt atgattcata taataacttt gaaatctggc ggaaactcct gatttgccaa
      121 cccgtctctg aaatcccccc cgcccgcctt taaaaaatcc ccccccaccc ccctttttca
      181 aaggggggaa cggggggatt tttgcaggcg ccttgctcga gaggaatccg cgaagaaata
      241 gggtggattt ctttcaaaaa gttaaagcct tacgaaattc ggaactcgca acgggcaccc
      301 gggtctcatc ccagccaccg ttttctgcgc ttgtagtgct tgacctcccg gtagcttttc
      361 cgctttccta ccccgctcaa acccaggtaa aattccttga tatcctcatt atccttcagc
      421 ttagaggttg gcccgtcgag aaccaccttc ccgttctcca tcacgtatcc gtattcggcc
      481 acagaaagag cggccaccgc gttctgctcc accaggagga tggaagcctt ctcctcctgg
      541 ttgattcgcc gggcgatcct gaaaatctcc ctcaccagga ccgggctgag tcccagggac
      601 ggttcgtcca ggagcatcac cttgggatgg gccatgaggc cccgtcccat gaccaacatc
      661 tgccgctccc ctcccgaaag gtatccgctg gtcctgggac tgagttccat caggcgggga
      721 aaatactcat agaccctctg gaggtctcgt ttcaccgccg gtccatcccc ccgggcatag
      781 gcccccacga agagattctc ctccaccgtg aggtgttcga agagccggtg gccttccatc
      841 acctgtacaa tccccaggcg cacgatctct tccgggtcca ggcggtcaat cctctggccc
      901 tcaaattcaa tgctcccgtc ggttacttct ccttcttcgg tgtgcaggac cccggagacg
      961 gctttcaggg tggtcgtctt acccgccccg ttggctccca gaagagccac gatctttcct
     1021 ggggccacct ccaaggagac ccctttgagc accagaatta cgtcgctgta gatcacttca
     1081 atgttattca aacgaagcat ggaccccttc ccgcctcatc tattttcccg gaggaacaaa
     1141 ctcgatcttt gacagcgcgg tcctccccga ctccgccccg gtgatccgga aggtgtaaac
     1201 cccgtgggga atgggcttga aggatttggg atcaggagct agcgtcgggc tgatgtcccc
     1261 ccgcaggaaa gtcatgatct tggtcggagg agtgatatcg gcagtaaagt ctcccttttc
     1321 atcggcatat ccaaaggcca cccccaccgg ctctcccggc ttcaccgccg gaatctccac
     1381 tcccggtggg acttccatct ccaccacaac catttccttg ggctggtagc cagatcccga
     1441 gaaatagata cgcgtctgga ggatgctgag catggcgggc agggtcgtgg ggagagtcac
     1501 cttcgggggc ggaggttcgg gggccggcgc ggaggtcgca agctgctccg gcgggggcgt
     1561 tgtgcttttg caggctccca gtaaggagac gatcatgacc agatatatga accaagaaaa
     1621 tctcttcatt tcctttccct cctttcctta attattgtca gccacataag tagtttataa
     1681 aattttccag tctatttaga tttccccaga aattcccccc caccctatcc ccccctccga
     1741 gaggggaggg gagggagggg tgacttcttt tgcttgcggc tctttcgctc tgaactctgc
     1801 ggtgaaatgc tgttcagaat ttgtgcaaag agacggcatc gggggccttg atccaatccg
     1861 tgatggccac cgccctggag ttcttgacct ggtagaactt cacctccagg ctacccctgc
     1921 gtgacgttgg gctgtaggcg ttgatcccgg tgattccctg ccgcttgaac ccggcgagct
     1981 tctgataagc tttgaacatg gcctccccgt cgagtttctc ataccccaca tccttgaggg
     2041 cgatcttcag tccttcttca aagtccattc cccagaccat cccggccagg taggtctcgt
     2101 tgaaatcggt aatgggtttt ccgcgatatt tcgtccacag ggaagagccg ggatgattct
     2161 tggcatcttg tccgcgcagg tagtaggagg tgatgaccgt cccttccaat gcttcggggt
     2221 gcgcccgaat tcccaccggt tcggtgggtc cccagtaatc gttcaagaac tgaatggtct
     2281 tggtcaggcc gagcttgtgg gcgtcgcgga tgatgttggt cggcgtgggg tcaacccctc
     2341 cggcaaaaat ataattggct cccgcgccgg ccaggcgggt caggtacacg gtgtgatcca
     2401 gcgcccccgg gggaaagaac tctggcttga ccatgggcag gccgatcttg tcggcgtact
     2461 catttccccc tctgagaaac tctttcccgt acgggctgtc ccagctgatg aaaccgaact
     2521 tgggcatccc cggcttccct ttggctttcc agtccattac cacccagtcc atggcggcgg
     2581 aaaaagcatc ctggtaggcc gggccccaga tgaaaatccg gccgagcttg gcctggaact
     2641 ccccatcccc cggaacgagc tggaccagcc tgtctttcgt cgccaggggg gccacggcct
     2701 tgccgatggg cgtgccgata gcgttgacca ccaggagctt atgctcggtc cggtatcgtt
     2761 tgtaagccga aactccccgg gccacgtcgt agcgggtgtc cacgccaatg aatttgacct
     2821 tcactccatc cacccctccc cgggcattca ggtctttgaa ataatcttcc acccccatat
     2881 cggccgggac attcaggccg gcaatggccc ccgtataatc ggccaggctc aggtaggtaa
     2941 ccattttctc ctgggcccaa gaatcgccct ccagggtgca gagcaaaatg catagagcca
     3001 aaataacgaa aatgaaccct cctcctgctt tcctcataat gccctccttc ctctaaagtt
     3061 tcagcctcta cgggcaggtt tcgaacccgc ccttgcactg ctatcaatac gcaaacggat
     3121 agagccggta agaggtcttg aaaatctccc agcgatgggc gatcccccgg ggttcgaaga
     3181 tgagaaagac catgaggatc aggccgaagg cggtgatccc cagggaagct gccggatggg
     3241 ttcccagcca ggggaaggtc gatgccagga agggagacga gaacatgacc agttcgtcca
     3301 gtccccgcag aaagatgacc ccgaagaaag ccccggcggt cagactcatc ccccccacga
     3361 tcagcatccc cacgtaccag acggcgtgga gcacggtgaa ctgctccgga tggaccacct
     3421 ggatccaatg agcccataga gagccggcaa ctccggcata gaagcacgaa atgaagaagg
     3481 ccagcagctt gtagcggtag agattgattc ccatgacctc ggcggccagg tcgttgtccc
     3541 gaatggccac gaaggcccgc cccaggcggg tgcgcatcat attcttggcg ccgtaggtca
     3601 tgagtagcat caccgacacg atgatgtaat acatcctccc gtcgctgtcg aattggaatt
     3661 ccccgatgct gggcggggcc ggatggaggc cctgggtgct cccggtgatc tccaggtgca
     3721 tgatcagcca catgatcaga aaatgaatgg ccagggtggc catggccaga taaaagccct
     3781 tgattcgcaa tgaaggggct cccccgatca ggccaatgac cccggcggaa atgccggata
     3841 agggcaaagc gagccagaag gtgactccga atttcagggt tagaatggcc gaggtgtagg
     3901 cgccgatggc catgaaagct gcctgcccga aggagacctg accgcagaac ccggtgacca
     3961 cctgcagtcc caggacaatg atgaaggtga tgcagaaata attgatgagg ctgatgaagt
     4021 attcctggcc gaaggcggga aagagataaa gaaggaataa ggagatcagg agaatcgccc
     4081 agtgccgctt cgtccggaca atggccatgt cctgcgcgta ccgctcgttg tacactccac
     4141 aaggaagcat ctcaaatcct ttcgatccgt tttaatccga agaggccaaa gggtttaacc
     4201 agcagaatga ggagcaacat cacataggcc gccacatccc ctacccctcc gccgaccaga
     4261 gggtccaggt atcccgcgct caaattctcg agcattccca ggatcagccc ggccaagagc
     4321 gcgccgggca gcgaatccag gcccccgaaa agggccacca ccaacccttt cagccccacc
     4381 cagggtaagg ggtagtgaat gtccatcacc cgggccaggg agattccggc caccgcggca
     4441 accagggcgg aaacgatcca gacgatggag aagatcgtcc gcaccgagat cccagtggac
     4501 tgggccagtt ggtggtcctc cgccgtagcc cgcattccca gcccgatttt ggtgaagcga
     4561 aaaaggagcg ccagcaggaa gaacaaaccc agggccacgc cgaagcttaa aatgcccccg
     4621 gtgggaatgt tcagcccacc tacagatagg gtttctgtgt tcaagaaccg ggggagggtt
     4681 cgggtctctc ccttgaggat gagctggaat atcccgtcca tggtcatgaa gatggcgaag
     4741 gtcatcagga aagcggagaa gaggggttgg ccgatcagag gacgcaggga gaagcgctcc
     4801 gccgccaggc cgatggcccc cgccactagg aaggtcagaa ggatagcagc ccagaacggc
     4861 agggccagga ccacgatgaa gacgtagaag aaaaaggccc cgaaggccag aaactggccg
     4921 tgagccaggc tgaccacgtg ggtggactta tagaccagga cgatggccgc ccccaccatg
     4981 gcgtacagtc caccggtcag caatccggtt atggccagct ggagaaattc agtcatccag
     5041 tcccctcatt tattttattg gacccagatg aacgaagatt ttcaggatta acaaaaaagg
     5101 aataaatttt aattctttgt ttttttaata gctttgtggt cataaacgac cctcctggaa
     5161 atccctcccg gcctcccttt tccaaaggga ggagaggtta tttacgggaa ctcgatgaat
     5221 cacgaggcct tttccccctt tgaaaaaggg ggattaaggg ggatttcgcc ttttttcaga
     5281 acgcaaaatt gttacttttt tttataaaaa tcgatctgcg ttgatctgcg aaaatcttcg
     5341 tcccattaat ttattttatt tatgtgaatg gcggtcttga tcactccttt tctcccgtcg
     5401 cggtaggtga tcgccgcttc caccgcgtat tccgggctgt ccccatacag agccatcacc
     5461 aggtctttgt accggtcttc cacgaaggtc cgtcgcaact ttcgggtccg ggtcagctcg
     5521 gcctcatcgg cgtcgaactc tttgtgcagg ttgacgaagc gctttacccg ggcataatcc
     5581 ggcagggtcc ggttgacttt ctccagctcc ttcctgacca gctcgacgat ttcgggtttc
     5641 tgcgacagat cggtgaaggt ggtataggga atccttttgc tctccgcgta gcgccccacg
     5701 ttctccaggt cgatgttgat gagcgcggtc acgaattccc tatcctctcc tccgaccacc
     5761 agaacatctt tgatgtaggg gctgaaacga aggcgcacct cggtgtactg cggggaaaat
     5821 ttcttgcccc cggccagcgc cttcaggtct tccatccggt cgatgacaat caggtgtcca
     5881 ttttcgtcga tatgcccgaa atcgcccgtg gcgtaccatc cgtctttcaa cttccgggcg
     5941 gtggcctcgg gatccttgta atagccggag tacataaact ggctcttaac cagaatctct
     6001 ccctcatccg aaagcctgac ctcggcccag gggactacgg tgccggaagt ttcggggcgg
     6061 atccctctcc cccgcggaac cgaaacgacc cccagctccg tgctcccgta aaaaagcttg
     6121 atctgaacat ccagggcctg aaagaagcga atgatctccg ggctgaccgc tgcccctgcg
     6181 gaatacacgc acctggcatt ggagaggccc agcttgtctt tgagcgcccg gaagacggtc
     6241 tggtaggcca ggacgtacaa gacccggtcg agccaagagg gtgttttccc ggccccgagg
     6301 atatccgcca tccggtagcc gagagggagg cagagccggt agagaagccg gcggagggca
     6361 gtggaatcta tcatccgggc ctgcaccatt cggttcacgt tctcccagag ccgtgcacca
     6421 tagaaaagaa tctgcggccc gatctcccgc aggttctcct ggacggtctc cggttcttcg
     6481 gggaaattca cggacacgtc cgccaccagg gagccgccga tccccaggcc ctgctccgtc
     6541 gcccaagcgg gagggatgaa ggaaaggtac tggtaccctt tttcgttcca gtcatccatc
     6601 aaggcccagg cccgaactcc gtcaaccagc cacctctggc tgagcatcgc ccctttgggc
     6661 aggccggtgg ttccggaagt ataacagata accccgatgt ccgagctctg cccctggtcg
     6721 attttttgct cgaagagctg gggatttacc ttctcctggg ctgcgccgag tttcaggacc
     6781 tcctcgaagg agaaaagcag cggatcgtgg taatgccaga gtcctttcgg gtcccagaag
     6841 atgacctttt tcaggagcgg aagctgtcct ttgatctcca ggagcttgtc cacctgttcc
     6901 tggtcgtggg ccaccacaaa tttggagtcg gagtgctcca ggtaatattt cacttccgag
     6961 gggatgcagt cggtgaagat ccctactacc gtggccccgg cacagtgagc ggcaagctcg
     7021 gcccagtacc attccggctt gttctccccg agaatggata ccttgtctcc tcgctccaag
     7081 cccagcttca tgagtccgag ggaaaggtat ttgaccttct cgtaataatc cttccaggtg
     7141 tagctctgcc agatgccccg atccttcacc cgcatggcca tcttgcggtc tccatgcttc
     7201 cgataattcc accgcaggag cttagggagg gtatccagat tgcggggagt ttcctcttgg
     7261 ctaaccaatc tccccctccc ccaggtaggc tttgatgacc cgcgggtctt gtttgatatc
     7321 cgccggagtc ccctcggcga tcttctggcc gaaatccagg acgatcaccc gctgggcgat
     7381 gtccatgacc actcccatgt catgctccac cagcgcgatg gggacccgct tcagctccga
     7441 aatgtcgatg atgaaccggg ccatatcctc cttctcttct acgttcattc cggccatggg
     7501 ctcatcgagg agaagtattt tcggctctag cgccagggcc cgccctagct ctacccgctt
     7561 gcgcaggccg taggggagca ggcccaccgt tttcttccgc accggctcca gttccaggaa
     7621 atcgatgatg tcttcgacca ctttccggtg ggcgatctcc tcccggcgca tggggccgaa
     7681 atagagaaat ccggccaggg ccccgcggcg catgtggatg tgccgcgcgg ccatcaggtt
     7741 gtccagcgtg tttaggccgg tgaagagttc gatgttctgg aaggttcggg agatgcccaa
     7801 ttttgctatg cggtggggag acaaaggagt gattctctcg ccctggaaga aaacctctcc
     7861 ccgctgaggg cggtagaatc cgttaatgca gttcagggtg caggtcttgc cggccccgtt
     7921 tggcccgata atggccagga tctcggcatc tctcacctcc aggctcaccc cggagagagc
     7981 catcatcccg ccgaagcgga gatcgaggtc gtccaccctg agcacgatct ggtctccccc
     8041 gcctgtcgga tgggaagctg gcatcgcctg ctccttctag gtttcgtgga cctcggttca
     8101 gcaacgcttc aagaacgatg gaaaaaacca atctgggagt cgatccgtac ggaaaggaaa
     8161 aatatgtgaa aagctacgac taaaggtatc ctgccctctc tatgcggtac catatgtaac
     8221 aagatcctgg aaggttgtca aggaattttg cttcactttc cggcggggca ttttttctcg
     8281 cagttcctgg aaaggtccaa agtctcgtct gcccggattc ttcttggcgg ttgaaacttt
     8341 cactcctgca tggtaaataa agattgagtt attttctcct cgaaacgatg gcaagggaaa
     8401 gaaagaaccc gtttgataag gagatcatgc cgtgaagatg cgatgggaaa ggaccccggg
     8461 ggggacctta atcgcgatgg cttttctctt cactctcctt ttcctgggct gcgcccgaac
     8521 ccggcagccg gtgtccgagc cagttctccc gaagccggag acttccgagg tagtgtctga
     8581 ggtccagaaa accgaccggg tcatggttcc ttctgcctct gccgttccga ctcctcccac
     8641 cccgcagccc gagccaccga tccctgagcc accgatcccc gagccgcagg ccgccccgaa
     8701 gccgaactca ggggccgaag aagccgcgca agaccggatt gcttcaggag ccgtgctcct
     8761 caacccttct gtgttcgaag atgccaaggc catccagagt cgactggcgg aactcggggt
     8821 ttacaagatg gccatcgatg ggatgtgggg aagggattct cgcgccgccc tgcgtttttt
     8881 caaggaagag aacaacctga agttccccga ccagtgggac aaggaaaccc aggtagctct
     8941 ctttggaaaa gggggcgctc cggggcctcc ggttgacacg agcgatcccc tcgccagcgg
     9001 gacccttctc ctgaccccgt ccaacccgga ggacgtcgag acgatccagg gccgactggc
     9061 cgagttgggg ttttactccg ggggaattga tggaatctgg ggcagagaca cccgagccgc
     9121 tctgcgggct tttaaagaaa agatcggtct gaaaaatccc gagcagtggg acaaagagac
     9181 ccaaatgctc cttttccgcg agttagggaa atggccctga tcgaaatctt tgcgccctgg
     9241 aagtgctgcc gggttaaaac cttgaggaga atgacttcta cccatcgact ctttttttaa
     9301 aacagattcg caaaatcaaa ccatcctgtg aaccctgatt ctcccctcaa cttcaacacc
     9361 tatcccagcc agggaaaatg aattcaaaaa atacttgaca gtcatgatcg gggatgataa
     9421 tcatccataa tcgcatttct tcttttcggt taaccttttc tttttcatat aaatatttca
     9481 gagggagaga tagggataga gattttttta tccgagatca tttttttgtc tccctctttg
     9541 atcttccgta aaggaggaga tcatgagaat ccgggaaata ttctctttga tttctttttt
     9601 ggttctcgct ttttcaaccc ttcctttggc tgaggttatg gctcagccca aagagattgt
     9661 tttgggcacc gccttaccct taacaggggg gcagtctcgg gaagggggct atttcaagaa
     9721 aggctatgaa ttggcagtaa aagagataaa tgatgggggt ggaatcccga tcaaggaata
     9781 tggcaaaaat attcctatca aacttattat ttatgacgat aaaagcgaca atacgacctc
     9841 cgtccagctc tatgaaaagc tggtgaccca ggataaggtg aatgccctcc ttggcggcta
     9901 tggcacccct ctgatcaccg ctcacaccat agtggcggag aaataccggg tcccttactt
     9961 gaatggagga ggggccactg cggccattta ccagcggaac atgaaatatg tctttgggat
    10021 tctagccagc attgaaaagt tggcccatac cctcatggac tgggtctccg cccagcagga
    10081 tgcaggccat ctgaaaaaac ccctgaaaat ttccgtgatg ggagaaaaca cctcccacgg
    10141 caaggagttt cgccaggggg ttctggataa gagcaaggcg gctccagctc gtttccagat
    10201 cgtaatggac gaaccctttg agctgaacct taaagatgcc gatcctcttt tgcagaaact
    10261 caaggcttcc gatgccgatg tatttttggc ggatgcccgc ctggccgatt acacgaccat
    10321 ccaccggcgt tatactgagc tgcgcctgta tcaccaaatc gtgagttacg gcccgcgggg
    10381 atcggagaag gcggctcgtg atgctctagg agaggcttcg gactacatcg tatcctgcaa
    10441 ttggtggcat aaggaggttc ccaccccagc ggccaggaaa tttgccgaaa aatatgctca
    10501 gacttaccat gaacccgcag aatggttccc ggcattggct tacgaaacgg tccgggtgat
    10561 ggccaaagcc atggaaatgg ccggaagcct ggataaatcc aaaatccggg atgccctggc
    10621 caaaatggat atgaaagact ccctcgtggt tggaggacgt gtctggttta a
//
LOCUS       DASPRR010000021         9713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2265057, whole genome shotgun sequence.
ACCESSION   DASPRR010000021 DASPRR010000000
VERSION     DASPRR010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2265057"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VEL68_00755"
     CDS             complement(<1..295)
                     /locus_tag="VEL68_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ34526.1"
                     /translation="MKSVKREQELPWSPHPFLPIQIKNFLSKRDDQADLTIFLVNLPA
                     GKEIPEHVHETQEDIIFILKGRAKMWIDGVGEFELGKGTFVRVPQNTKHRIYNV"
     gene            857..1096
                     /locus_tag="VEL68_00760"
     CDS             857..1096
                     /locus_tag="VEL68_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXZ34527.1"
                     /translation="MEKRDLELIQKHISSDAELKRYMDEHEEYERKLQAFNQRIYLTP
                     EEEIERKRLQKLKLIGRDRIEAILSKYRAQKMGEG"
     gene            1099..2799
                     /gene="ilvB"
                     /locus_tag="VEL68_00765"
     CDS             1099..2799
                     /gene="ilvB"
                     /locus_tag="VEL68_00765"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HXZ34528.1"
                     /translation="MKKTGAEILFESLKQEGVDTIFGLPGGAVLTLYHALGKYAEEIR
                     HILVRHEQGAVHAADGYARSSGKVGVCLVTSGPGGTNTVTGLATAYMDSVPLVVFTGQ
                     VPTMLIGNDAFQEADITGISRPCTKHNYLVKDVKDLAMIIKQAFHLARTGRPGPVLVD
                     LPKDVVADSTEFSYPSKLKMRSYNPTYQGHPGQIKRAAKLIASAKKPLIYAGGGVILS
                     NSSKELTTLGQKLSIPVTTTLMGLGGFPGSHPLFLGMLGMHGSFHANMAVTHCDVLIA
                     IGARFDDRVTGKVESFSPHSKKIHIDIDPTSISKNVPVDIPIVGDARNILKTLLKYLE
                     EETGQDWAKKHSEWLQQIAEWKNNYNSTPQDSHRGPCIKPQFVIQKINEITKGDVIIA
                     TEVGQNQMWAAQSFLFTKPRTLLTSGGLGTMGYGFPAAIGAQVAFPDKTVIDIAGDGS
                     IQMNIQELATAVQYKLPVKVAILNNHCLGMVRQWQQLFYGQRYDQSVFEVVPDFVKLA
                     EAYGAVGLRATKPEEVEPVIRQALSIAKPVFMDFLIDQNECVYPMVPAGAALTEMLLV
                     "
     gene            2808..3332
                     /gene="ilvN"
                     /locus_tag="VEL68_00770"
     CDS             2808..3332
                     /gene="ilvN"
                     /locus_tag="VEL68_00770"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXZ34529.1"
                     /translation="MRHVISVLVENEPGVLVRVSGLFSGRGFNIESLNVAETLDPTIS
                     RMTLVTSGNEQIIEQIIKQLNKLVNVVKVVDLTGSDFVDREMILIRVKAEVSSRAEVL
                     RILDIFRGRVVDVAPKSYTLEITGDEKKVQAVIELLAPFGILEVVRTGKVAISRGKKE
                     ALGIGRAREEKAEG"
     gene            3453..4511
                     /gene="ilvC"
                     /locus_tag="VEL68_00775"
     CDS             3453..4511
                     /gene="ilvC"
                     /locus_tag="VEL68_00775"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXZ34530.1"
                     /translation="MAKISFGGVVEDVVTREEFPLSKAQQVLKNELVAVLGYGVQGPG
                     QSLNMRDNGIRVIVGQRKNTPSWDKALKDGWVPGQTLFPLEEASAKGTIIEYLLSDAG
                     QKEFWPKLKPHLTEGKTLFFSHGFSITYRDLSGVIPAKNLDVILVAPKGSGLSLRRNF
                     LEGSGINSSYAIFQDYSGKAKEKTLALGIAMGSGYLFPTTFENEVFSDLVGERGVLMG
                     AIAGIMEAQYNELRKHGHSPSEAFNETVEEFTQSLIKLAAEKGMDWLYANCSTTAQRG
                     ALDWKGKFRDTVAPLFSQLYQRVADGTEAKIVLEKNTQPDYRQKLDAELAQMRNSEMW
                     QAGEKVRELRPENWKKEA"
     gene            4616..6244
                     /locus_tag="VEL68_00780"
     CDS             4616..6244
                     /locus_tag="VEL68_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ34531.1"
                     /translation="MPNNQPFITLEHVTLRLPNRLILEDSSWRVQADEQWAILGPNGS
                     GKTTLVRSLWGGVPLRSGKMAFDFLGSGSQDGDFPPLEAIGYVSLEMHQRLMEQEELQ
                     EEFRAYAEKEGEGTTAREVVFSGILASRPLRQEDETRLSEVSLLLEISQILSRPIRSL
                     STGETRKIIIARALMKSPRLLILDEPFEGLDEKGRDLLARSINGLMTGPMRVILVAHR
                     LEEVVPNITHVLLVKGGRLFLQGPKEEVLTSENLSQLYGCSLRLGKNNGTYFLNYGEE
                     ESTSKELPGEYRGGFSEESEGLIEIRDTTVRYGDLVVLDRVNWAMGRGENWAILGPNG
                     AGKSTLVKLILGENLQAYANPIFLFGQRKGSGESIWDIKKRIGVVSSELQVQYRKRIS
                     AFEVIVSGFYDTIGLYQAPTSTQQEVASRWVDFLDIKELAKEPFNQLSFGQRRMILLA
                     RAMVKSPVLLIADEPCHGLDVPNRIKILRVLEKIGRTNTRLLYITDRSEEILNCITHV
                     MRLDMGKVVQQGRKEEVVGAGFKPSLTPGKEPVV"
     gene            6269..6919
                     /locus_tag="VEL68_00785"
     CDS             6269..6919
                     /locus_tag="VEL68_00785"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HXZ34532.1"
                     /translation="MNSEIRKLPVAKEGMIFILPLGVLSLLLWGFRFPVLAAFCTFLT
                     LFVVYFFRDPERGAPPGEKAILSPADGKVIQVQPCLEERFLGGPAIKVSVFMSLFNVH
                     VNRNPLSGKIVDSSYSSGEFLRADRDRASAANEQNALLVETAEGARLLVIQIAGLIAR
                     RIVCRVKKGDWVVRGQRFGLIRFGSRLDIYLPRDTQLQVRVGQKALAGQTILGHLP"
     gene            6916..7698
                     /gene="pssA"
                     /locus_tag="VEL68_00790"
     CDS             6916..7698
                     /gene="pssA"
                     /locus_tag="VEL68_00790"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXZ34533.1"
                     /translation="MRREKKERLKKMHRMRKGIYILPNLITSGSLFCGFFALLRTLQE
                     DFYTAAVFILASGLLDGMDGKIARYTNTTTRFGVEFDSLADVIAFCVAPGILVYAWAL
                     EPFGRMGWLAAFLFVVCGALRLARFNLQGSTVESRFFSGLPTPAAAGLIATGILVYYE
                     AGDTGVDKHLTVLIATYLLAFLMVSTFKYYGFKDIELFRRKPFRWLVIAILLLIVIAY
                     EPEYTLFGLFFAYFISGPILSLVLLFYRRRHPKPVMPAGKMA"
     gene            7763..9343
                     /locus_tag="VEL68_00795"
     CDS             7763..9343
                     /locus_tag="VEL68_00795"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXZ34534.1"
                     /translation="MNAADESSPHFARKVVSAMERVLIFDTTLRDGEQSPGASMNLGE
                     KILFARQLERLNVDIIEAGFPASSPGDFESVKRISQEVRGPQIAGLARTTPKDIDTAW
                     EALKHARAPRIHVFIATSDIHLKHKLRKSREEVLEEAGRAVAHAKRYTENVEFSCEDA
                     TRSDPDYLARVLEAAIDAGATTVNIPDTVGYTTPTEYAKLLTYLRQKAKNIGKAVISV
                     HCHDDLGMAVANSLTAIENGARQVECTVNGIGERAGNASMEEIVMALKTRKDFFSFEV
                     GIITEQIYPSSRLLTSITGIQVPPNKAIVGANAFAHESGIHQDGVLKEKITYEIMKPE
                     TVGISQSTLILGKHSGRHAFRDRVRSLGYELSEAHLNQAFQRFKEVADKKKQVFDEDL
                     EAIIADEILRIPDFYKLVNMNVLSGSTTVPTATVQIEIGGKLQQEAAFGDGPVDAAYK
                     TISKITKTKSHLVKFSVNAITSGTDAQGEVMVILSEGGIRVTGQGAHTDIIMAAAKAY
                     INALNKLEYQKRLKGVSS"
     gene            9340..>9713
                     /locus_tag="VEL68_00800"
     CDS             9340..>9713
                     /locus_tag="VEL68_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXZ34535.1"
                     /translation="MIRPMTITEQILAEHAGQHEVEPGQLINAKVDLVLANDITAPIA
                     IEEMRKIGATQVFDPAKIVLIPDHFVPNKDIKSAQQCQLMREFARELRLPNYFEVGEA
                     GIEHAFLPEQGLVLPGDLVVGA"
BASE COUNT         2192 a         2746 c         2764 g         2011 t
ORIGIN      
        1 tcacgttgta aatccggtgc ttggtatttt ggggaacccg aacgaaggtt ccttttccca
       61 gctcgaactc ccctacccca tcgatccaca tcttggctct gcccttcaat ataaagataa
      121 tgtcttcctg ggtttcatga acatgctcgg gaatctcttt gcctgccggg aggtttacga
      181 ggaagatggt aaggtctgcc tggtcgtccc ttttgctgag aaaatttttg atctggatgg
      241 gaagaaaggg gtgaggactc catggcagtt cctgctctcg tttcaccgat ttcatgcagc
      301 ctcctttttt taaagaatca ggcgatttaa gggtatggcc ggttcgcagg taggttgtga
      361 aggcgttcct ccaacccttc tgtccctctg cgacctattt tgtattatag tctattcact
      421 ccctttgcca aagcctttga gttttcaaac ttgcccaccc ccagttctta aaaaagagtc
      481 cttcgaaaat aagacctctg gaaaagaggg gggatctcat gggcttcagg gcggagaaat
      541 ttggatcatc atcctgaagc ggtggttctt ccatgggatc gggcctgtcc tggggggttg
      601 aaacgaaaaa atgctttggt tctcttttct gcttcgaggt atctcctctc aggcctctcc
      661 ttgcggaaca ggggaaggag tttcatggaa ggataagcac ccgccgagcc tgaagatcca
      721 aattcctcca ccctgaaccc caacaagcac gaaaacctgg gggtaggcaa gagttttcaa
      781 aagattgact ttcaaaaata tttggaatat tatggaattt catcatttta ttcgagcatg
      841 gcaggaggcc gggaccatgg agaaacggga tctggaactc atccagaagc atatctccag
      901 cgatgcagaa ttaaaacgat acatggacga acatgaggag tacgaacgga aactccaggc
      961 cttcaaccag cgaatctatc tcaccccgga agaagagata gaacggaaaa ggctccagaa
     1021 actgaagctg atcggccggg atcgtatcga ggccattctt tccaagtatc gggcccaaaa
     1081 aatgggggag ggctaaggtt gaaaaaaacg ggagcggaga ttctgtttga atctctcaaa
     1141 caggaaggcg tggataccat cttcggtttg cccggtggag ccgtcctgac tctgtatcat
     1201 gccctgggca aatatgccga ggaaatccgc catattctgg tgcggcatga gcagggtgcg
     1261 gttcatgccg ccgacgggta cgctcgatcc tcggggaaag tgggggtatg cctggtgacc
     1321 tctggcccgg gagggaccaa taccgtcacc gggctggcca ccgcctacat ggactctgtc
     1381 cccctggtgg tcttcaccgg tcaagttccc accatgctca tcggcaacga cgccttccaa
     1441 gaagcggaca ttaccggaat ctcccgcccc tgcaccaagc acaactacct ggtgaaagac
     1501 gtcaaagacc tggctatgat catcaagcaa gccttccatc tggcgcggac cggaaggccg
     1561 ggcccggtcc tggtggattt gcccaaggac gtagtcgccg acagcacgga attcagctac
     1621 ccttccaagc tgaagatgcg cagctacaac cccacctacc agggccatcc cgggcagatc
     1681 aagcgtgcgg cgaagctcat cgccagtgcc aagaaacccc tgatctatgc cggagggggg
     1741 gtaatcctat ccaattcttc caaggaactt accaccctgg gccagaagct ctccatcccg
     1801 gtcaccacaa ctctcatggg cttggggggc tttcccggga gtcatcccct cttcctgggc
     1861 atgctgggga tgcacggcag ctttcatgcc aatatggccg ttacccattg cgatgttctt
     1921 atcgccatcg gcgcccgctt cgatgaccgg gttaccggga aggtcgagtc cttctcccca
     1981 cactccaaaa aaatccatat cgatatcgac ccaacctcca tctccaaaaa cgtcccggtg
     2041 gacattccca tcgtggggga cgcccgaaac attctcaaga ctctgctgaa atacctggag
     2101 gaggagaccg ggcaggattg ggccaaaaag cattccgaat ggctccagca aatcgccgag
     2161 tggaaaaaca actataattc cacaccccag gactcccatc gcggcccctg catcaagcca
     2221 caattcgtga tccagaaaat caacgagatc accaaggggg acgtgatcat cgccacggaa
     2281 gtgggccaga atcagatgtg ggccgcccag tcttttctgt tcaccaagcc ccggacgctg
     2341 ctcacttcgg gagggctggg aaccatgggg tacggtttcc ccgcggccat cggggctcag
     2401 gtggccttcc cggacaaaac ggtgatcgac attgccggcg atggcagcat ccagatgaac
     2461 atccaggagc tggccaccgc cgtgcagtat aaactcccgg tcaaagtggc cattctaaac
     2521 aaccactgtc tgggcatggt gcgccagtgg caacagctct tctacgggca gcgttacgac
     2581 caatcggtct tcgaggttgt tcccgacttc gtgaagctgg ccgaggctta cggagccgtg
     2641 gggctgaggg ccaccaagcc cgaggaagtc gagccggtca tccgccaggc cctctccatc
     2701 gccaagccgg tcttcatgga ttttctgatc gaccagaatg agtgcgtgta ccccatggtc
     2761 ccggccgggg cggccctgac cgaaatgctc ctggtgtagg gggaaccatg cgacacgtca
     2821 tctccgttct ggtggaaaat gagcccgggg tgttggtccg ggtttcagga ctcttcagcg
     2881 gccggggatt caacatcgaa agcttgaatg tggccgagac cctggacccc accatctccc
     2941 ggatgaccct ggtaaccagc gggaatgaac agatcatcga gcagatcatc aagcaactga
     3001 acaagctggt caacgtggtc aaggtggtcg atttgacggg gtccgatttt gtggaccggg
     3061 agatgattct gatccgggtc aaagccgaag tctcttcccg ggccgaagtc ctgcgcatcc
     3121 tggacatctt ccggggtcgg gtggtggatg tggcccccaa atcctatact ttagaaatca
     3181 ccggcgatga aaaaaaggtc caggcagtca ttgaacttct ggctcccttc ggcatcctcg
     3241 aggttgtgcg cacgggcaag gtggccatca gccggggcaa aaaagaagct ttggggatcg
     3301 gcagagcccg ggaggaaaaa gcagagggtt gaccatcgat ccatcccctt gggaaacacc
     3361 gacctctaac ccaagaaacc caattcccag aaaagctggc gggaagcgca caatgagcgg
     3421 gatcgatacc cattcatgaa aaggaggata acatggcaaa gatcagcttc ggcggagtcg
     3481 tcgaggatgt cgtgacccgg gaggaattcc ccctatccaa agcgcagcag gttctcaaaa
     3541 atgaattggt agccgtcctc gggtacggcg tgcagggccc aggacagagc ttgaatatga
     3601 gggacaacgg aatccgggtg atcgtcggcc agcggaagaa cactccttcc tgggacaagg
     3661 ctctgaaaga cggctgggtc cccggtcaga ctctgtttcc cctggaagag gcctccgcca
     3721 aaggaacgat cattgaatat ctgctctccg acgcggggca gaaagagttt tggcccaagc
     3781 tcaaaccgca cctgaccgaa ggaaagactc ttttcttctc ccacgggttc tccatcacct
     3841 accgggactt gagcggggtc attcccgcga aaaacctgga tgtgatcctg gtggccccca
     3901 agggctcggg cctttccctg cgccgcaact tcctggaagg aagcgggatc aacagcagct
     3961 atgccatttt ccaggactac agcgggaaag ccaaggaaaa gacccttgcc ctggggatcg
     4021 ccatgggctc cggttacctc ttccccacca ccttcgagaa tgaggtcttc agcgacctgg
     4081 tgggagaaag aggggtcctc atgggagcca ttgccggaat catggaggcc caatacaacg
     4141 aattgcgcaa gcacgggcac agccccagtg aggccttcaa cgaaactgtg gaggagttca
     4201 cccagagcct gattaaactc gccgccgaga agggaatgga ctggctgtac gccaactgca
     4261 gcaccaccgc ccagcggggg gccctggact ggaaggggaa attccgggat accgtggctc
     4321 ccctcttcag ccaattgtac caaagggtgg ccgatgggac ggaggcgaag atcgtgctgg
     4381 agaaaaacac tcagcccgat taccggcaaa agctggacgc cgaactggcc caaatgagaa
     4441 actcggagat gtggcaggcc ggcgaaaagg tccgcgaact ccgaccggag aattggaaga
     4501 aagaggctta aatacagttt cgggttccga gtttcgagtt tcaggttaaa gactttacaa
     4561 gaagttgtcc attctttgaa ctcgcgactt gaaacccgaa actcgaaact tgattatgcc
     4621 caacaatcag cccttcatca ccctcgagca cgttaccctt cgcctcccca accgtctcat
     4681 cctggaagac agttcctgga gggtccaggc ggatgagcaa tgggccattc tgggtccgaa
     4741 cggatcgggc aagaccactc tggttcgttc tctctgggga ggagtccctc tgcgcagcgg
     4801 gaaaatggcc tttgacttcc ttggctccgg ttctcaagac ggagattttc ctcccctcga
     4861 agctatcgga tacgtctccc tggaaatgca ccagcgtctg atggagcagg aggaactgca
     4921 ggaggaattc cgggcctacg cggaaaaaga gggagaaggc accacggccc gggaagtggt
     4981 tttttcggga attctggcca gccgccccct ccggcaggaa gacgagaccc ggctctccga
     5041 ggtgtccctc ctgctggaaa tttcccaaat tctctcccgg cccatccgat ccttgtccac
     5101 gggtgagacc cggaaaatca tcatcgcccg ggccctgatg aagtcccccc gtctgctgat
     5161 cctggatgag cctttcgagg gcctggacga aaaagggcgg gaccttttgg cccggtcgat
     5221 caatggcctc atgaccgggc cgatgcgggt gatcttggta gcccaccgtc tggaggaggt
     5281 cgttcccaac attacccacg tgcttctggt caaaggggga cgcttgttcc tccaaggccc
     5341 caaggaagag gtcctgacct ccgagaacct gtcccagctc tatgggtgca gcctccgtct
     5401 gggtaaaaat aacggcactt actttctgaa ttatggagag gaggagagta cctccaagga
     5461 acttccggga gaataccggg gagggttctc cgaggaatcc gaagggctta ttgagatcag
     5521 ggacaccacg gtccgctatg gcgatctcgt ggttcttgat cgggtcaact gggccatggg
     5581 gcggggagag aactgggcca tcctggggcc gaacggggcc ggcaaatcga ctttggtgaa
     5641 gctcattctg ggggagaacc tccaggccta cgcgaacccc atcttcctct tcggccagcg
     5701 gaagggctcc ggggagtcga tatgggatat caagaagcga atcggggtcg tctcctccga
     5761 gctccaggtt caataccgaa agcggattag tgcctttgag gtcatcgtct ccgggttcta
     5821 cgatactatc ggcctgtacc aggctcccac ctccacccag caggaagtcg cgtcccggtg
     5881 ggtggatttc ctggacatca aagagcttgc caaggagccc ttcaatcagc tatccttcgg
     5941 gcaaaggaga atgatccttc tggctcgggc catggttaaa agtccggtgc tcttaatcgc
     6001 cgatgagccc tgtcatggcc tggatgtgcc caatcggatt aaaattttgc gggtgctgga
     6061 aaaaatcggc cggacaaata cccgtcttct ctatattacc gatcgttcgg aggaaatcct
     6121 gaattgcatc acccatgtca tgaggctgga catgggcaag gtcgtccagc aggggagaaa
     6181 agaagaagtc gtaggggcag ggttcaaacc ctcccttaca cccggcaaag aaccggttgt
     6241 ttagccgggc tggaatccat caaagaatat gaattccgaa atccgaaaat tgcccgtagc
     6301 caaagaaggg atgatcttca ttcttcccct tggggtactt tccttgcttc tgtggggctt
     6361 ccggttcccg gttctggcgg cattttgcac ctttttgact cttttcgtcg tctatttttt
     6421 ccgggacccc gaacggggcg ctccccctgg ggagaaggcc atcctgtcgc ccgccgacgg
     6481 gaaggtgatc caggtgcagc cctgcttgga ggaacggttc cttggggggc cggcgatcaa
     6541 ggtcagcgtg tttatgtccc tcttcaacgt ccacgtgaac cgtaaccctc tttcggggaa
     6601 gattgtcgat tcttcttatt cgtcggggga gtttctgcgg gccgatcggg atcgggcttc
     6661 ggccgccaat gaacagaatg ccctgctggt ggaaaccgcg gaaggggccc ggctgctggt
     6721 tatccagatc gccgggttga tcgcccggcg gattgtatgc cgggtgaaga aaggggattg
     6781 ggtggtaagg gggcagagat tcggtttgat ccggttcggt tcccggctgg acatctactt
     6841 gccccgggac acccagcttc aggtacgggt ggggcagaaa gctctggccg gccaaaccat
     6901 cctgggacac cttccatgag gagagagaag aaagaacgcc tcaaaaaaat gcaccgaatg
     6961 cgcaagggga tctatattct gcccaacctg atcacctcgg gaagcctctt ctgcgggttc
     7021 ttcgcccttc tgcgcaccct ccaggaagac ttctacacgg cggcagtctt catcctggcc
     7081 tccggtctgc tggacggaat ggacgggaaa attgcccgtt acaccaatac gactactcgt
     7141 ttcggagtgg agttcgactc tttggccgac gtcatcgctt tctgcgtggc cccggggatc
     7201 ctggtctatg cctgggctct tgaacctttc ggcaggatgg gctggctggc ggccttcctt
     7261 ttcgtagtct gcggggccct gcgcttggcc cggttcaatc tccaggggag cacggtggag
     7321 agccgcttct tcagtgggct tcccaccccg gccgcggccg ggctcatcgc caccgggatt
     7381 ctggtctact atgaagccgg agatacgggg gtcgataagc acctcaccgt gctcatcgcc
     7441 acctatctcc tggcctttct catggtcagc acttttaagt attatgggtt caaggatatc
     7501 gagcttttcc gccgcaagcc attccgctgg ctggttatcg ctattctttt gcttatcgtg
     7561 atcgcctatg agcccgaata caccctcttt ggccttttct ttgcctattt catttccggc
     7621 ccgattctta gtctcgttct tctcttctat cgccgccggc atcccaaacc ggtgatgccg
     7681 gcaggaaaga tggcgtagag aaaagaggcc ggatcgactg caaggcagag ctcaaaggga
     7741 agaatcattt tccatttttc ggttgaatgc agcggacgag tcttcgcccc attttgccag
     7801 aaaggtagta tccgccatgg aaagagtcct gatcttcgac accaccttga gggacggaga
     7861 gcaatccccg ggcgccagca tgaacctggg agagaagatt ctcttcgccc ggcagctgga
     7921 acgtttgaat gtggacatca tcgaagccgg gtttcccgcc agctctccgg gtgatttcga
     7981 atcggtcaag cggatttccc aggaggttcg ggggccgcag atcgccgggt tggcccggac
     8041 gactcctaag gacatcgata cggcctggga ggctctcaag cacgcccggg ccccccgaat
     8101 ccacgtcttt atagccactt cggacattca cttgaaacac aagctgcgga agagccggga
     8161 agaggtatta gaggaagcgg gccgggccgt ggcccatgcc aaacggtaca cggaaaacgt
     8221 ggagttctct tgcgaggacg ccacccggtc ggaccccgac tacctggccc gggttcttga
     8281 ggcggccatc gacgccgggg ccacgacggt caacatcccg gacaccgtgg gctataccac
     8341 ccccacggag tatgccaagc tgctcaccta cctccggcag aaagcgaaga atataggaaa
     8401 ggcggtcatc tccgtccact gtcacgatga tcttggcatg gccgtggcca acagcctcac
     8461 ggccatagaa aacggtgctc gtcaggtgga atgtacggtc aacggaatcg gcgaacgggc
     8521 cggcaacgcc tccatggaag agatcgtcat ggccctgaag acccgtaagg acttcttctc
     8581 ctttgaggtg ggcatcatca ccgagcagat ctatccctcc agccggctcc tgaccagcat
     8641 caccggaatt caggtgccgc ccaacaaggc catcgtcggg gccaacgcct tcgcccacga
     8701 gtcgggcatc catcaggacg gtgtattgaa ggaaaagatc acctacgaga tcatgaagcc
     8761 cgagaccgtg gggatctccc agtccactct gattttgggc aagcactccg gccggcacgc
     8821 tttccgggac cgggtgcgga gcctgggata cgagctcagc gaggcccatc tcaaccaggc
     8881 cttccagcgg ttcaaagagg tggccgataa aaagaaacag gttttcgatg aggacctgga
     8941 ggccatcatc gctgacgaga tcctgcggat ccccgatttt tacaagctgg tcaacatgaa
     9001 tgtgttgagc ggctctacga ccgttcccac ggccacggtt cagatcgaga tcggcgggaa
     9061 gcttcagcag gaggcggctt tcggcgacgg cccggtggac gccgcctaca agaccatctc
     9121 caagatcacc aagaccaaga gccatctggt caagttctcg gtcaacgcga ttaccagcgg
     9181 caccgacgcc cagggggaag tcatggtgat tctatcagag ggtggaatcc gagtcacggg
     9241 ccagggggcc cacaccgaca tcattatggc ggcagccaaa gcctacatca atgccctgaa
     9301 caaactggag taccagaagc gccttaaagg agtttcctca tgatacgccc catgaccatt
     9361 accgagcaga ttctggccga acacgccggg cagcacgaag tggagccggg ccagctgatc
     9421 aatgccaagg tggacttggt tctcgcgaac gacattaccg ccccgattgc catcgaagag
     9481 atgcgcaaga tcggcgccac ccaagttttt gaccccgcca agatcgtcct gatcccggac
     9541 cactttgttc ccaacaagga catcaagtcc gcccagcagt gccagcttat gcgggaattc
     9601 gccagggagt tgcggctgcc caattatttc gaggtggggg aggcagggat cgaacatgca
     9661 tttctacccg aacaggggtt ggtccttccc ggtgacctgg tcgttggggc cga
//
LOCUS       DASPRR010000022         6981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_654312, whole genome shotgun sequence.
ACCESSION   DASPRR010000022 DASPRR010000000
VERSION     DASPRR010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654312"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VEL68_00805"
     CDS             <1..255
                     /locus_tag="VEL68_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXZ34536.1"
                     /translation="LKELGSRGIQSILLEGGPTLNASAWEEKIVDRVLLFIAPKVIGG
                     RGTPGMMGGEGVGRVKNARKVELLRVRRVGPDLMLEAKPL"
     gene            370..1035
                     /locus_tag="VEL68_00810"
     CDS             370..1035
                     /locus_tag="VEL68_00810"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXZ34537.1"
                     /translation="MFTGLIQDVGKVQAVEPAGGGVRLTVSTRLDIGLFKVGDSISVD
                     GVCLTVVRMSGSTFTVEVSPETLRRSTLATAKQSHPVNLETALRMSDPLGGHLVSGHV
                     DGTGEILEVEPEGNSWRYRFRVPREISRYLVEKGSVAVDGVSLTVVECGDEEFSVSLI
                     PHTAQSTTLGRKRAGDLVNLENDIIAKYVEKFLRQKEDPAQQPSRIDASFLARHGFKE
                     SME"
     gene            1041..2279
                     /locus_tag="VEL68_00815"
     CDS             1041..2279
                     /locus_tag="VEL68_00815"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HXZ34538.1"
                     /translation="MAVSRIEEALLDIRKGKMVILVDDEDRENEGDLCMAAEKVNPPA
                     VNFMAKYGRGLICLSLQEEKADELQLPPMVSNNTSSFGTAFTVSIEAKRGVTTGISAA
                     DRATTILTAIADEAKSDDLVRPGHIFPLRAKNGGVLVRTGQTEGSVDLARLAGLKPAG
                     VICEVMNDDGTMARMPDLEVFAKKHNLKIVTIADLIRYRLQNECMVRRAAITTLPTVY
                     GGEFTAIAYENDVDHQQHMALVKGEIRPEEPVLVRVHSECLTGDVFGSQRCDCGEQLH
                     TAMQIVAKEGMGVIVYMHQEGRGIGLANKIKAYALQDQGKDTVEANEALGFAPDLRDY
                     GLGAQILRDLGVRKIRLMTNNPKKIVGLQGYGLEVVDRVPLEIKPGRRNFGYLRTKKT
                     KLGHLLTLEKEGGTKNAKND"
     gene            2263..2739
                     /gene="ribE"
                     /locus_tag="VEL68_00820"
     CDS             2263..2739
                     /gene="ribE"
                     /locus_tag="VEL68_00820"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXZ34539.1"
                     /translation="MPKTTEGKLNAQGLRFAIVVSRFNDFICEHLLSGALDVLIRNGA
                     ADGDIEVFKVPGAFEIPQVAKKVVLSRKFDAVICLGAVIRGATPHFEYIAAEVSKGVA
                     MIGLEGEVPVAFGVLTTDNLEQAIERAGTKSGNKGWDAALSAIEMANLYREMKGKK"
     gene            2849..3295
                     /gene="nusB"
                     /locus_tag="VEL68_00825"
     CDS             2849..3295
                     /gene="nusB"
                     /locus_tag="VEL68_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXZ34540.1"
                     /translation="MTIRRRAREIALQVLYQLDIVPGDPQEALTLYFENFHPSEKTRE
                     FCRRLVEGVCQGQGEIDRLIEENAENWTLKRMAVVDRNILRLATFELLNCPDIPFKAS
                     LNEAIELAKKFGNDDSSAFINGILDQIRSLGGANLPDGTPLSKKPT"
     gene            3292..4020
                     /locus_tag="VEL68_00830"
     CDS             3292..4020
                     /locus_tag="VEL68_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXZ34541.1"
                     /translation="MRWAILSDVHGNLESFRAVIEDLRGEKVERVAYLGDVVGYGADP
                     NDCLTLLEGLTDWVVAGNHDYGSIGLTDIDVFNPSARSATLWTRDQLLPENRIYLRNL
                     LLRRQIGEITFVHATPNQPEDWNYIFTFPEAEEAFQSLTGGLAFIGHSHRPLILAKEK
                     DSEVRVIDHEEVILESGIRYIINVGSVGQPRDGHPEAAYGLYDETGKTYRLKRVDYDV
                     ATAQKKIIKAGLPSFLAQRLSRGI"
     gene            complement(4309..5436)
                     /locus_tag="VEL68_00835"
     CDS             complement(4309..5436)
                     /locus_tag="VEL68_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ34542.1"
                     /translation="MMISYPPPPVRPSILVLDGDYPGMDALVEEVLETFPLPCSGKKI
                     LLKPNMLGPYPQEKGITTHPSLIRSLVKALKKRGATCWVGDNPGMSGYAANERCARVS
                     GILEAADGGYVNFARETVQVPLKSPFLKKVVISRPVLEADLLINVPKFKTHVQTRITG
                     AVKNMFGILAGAEKARVHFAVPRPRQFSEALVDIYQVRVPDLTIMDAVVGMEGNGPSG
                     KELRFIGKVLASRNGVLIDGLIAAMMGIPAQDVDHLAVAGQRGLGEIDPARMEIQGSW
                     ESLKKFKTPIPFASRGWFGTVLNKLLYRPLVKPRLKVRRDSCTRCEVCIQHCPAQALS
                     LQGFPFLDEKKCISCYCCYELCPSQAMELTSWMRWATRPKK"
     gene            complement(5477..6829)
                     /locus_tag="VEL68_00840"
     CDS             complement(5477..6829)
                     /locus_tag="VEL68_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXZ34543.1"
                     /translation="MTEPYRLTLTEMIQQIRRRKLSPVALAESLLKRIDALEPSIQAW
                     VTLDRQKVLEEARRCEKETARGRKRGMLHGIPLGVKDIFYTAGVKTTGGSKVLADFVP
                     FFDSTAVLRLKQAGAIVLGKTATTEFAHADPAPTRNPWNREHTPGGSSSGSAAAVATG
                     MCPGALGSQTGGSVLRPASYCGAVGLKPTYGRISRYGVLPFSWSLDHVGLFTRTVSDA
                     ALLLNVLAGYDTQDPTTSREPVPDYTRASKSSRAPFIGVVEEFYRKNSETQVWKNTDE
                     VLARLKKTGAKAETVKMPGSFAVVHDAHRIVMRTEGAAFHQPLYEKHRELYRPKLREL
                     IEIGSLIPGVDYLTAYRIKREFRRQMDRVMERYDLLLTPSTSSPAPRGLESTGDAWFQ
                     VPWSFSGLPTITLPSGLSQEGLPLGIQLVGKAFSEGKLLALARWCEKALEISLTSVLE
                     "
BASE COUNT         1573 a         1888 c         2079 g         1441 t
ORIGIN      
        1 ctgaaggaac tgggttcccg ggggattcag tcgatcctcc tggagggggg ccctaccctc
       61 aacgccagcg cgtgggaaga aaaaatcgtg gaccgggtct tgctctttat cgcgcccaag
      121 gtgatcggcg ggaggggaac accgggaatg atggggggag aaggagtagg gagggtgaag
      181 aatgcgcgga aagtggagct cctgagagtt cggagggtgg ggccagacct gatgctcgag
      241 gcaaagcccc tttagggata gactttgcca aggaaatccg gagtgtgata aaatttgacc
      301 gtaggggtga ttacgccaaa ctcgtgacga ctcgcccctt ccatatggct gaactagggt
      361 cgggttgaca tgttcaccgg tttgatccag gatgtcggca aggtccaggc tgttgaaccg
      421 gcgggcggag gggtccggtt gaccgtttcc actcggctgg acattggcct atttaaggtc
      481 ggagacagta tctccgtgga cggagtatgc ttgaccgtgg tcagaatgtc cggcagcaca
      541 ttcaccgtgg aagtatcccc cgagacgctg cggagatcga ccttggccac agccaaacag
      601 agtcatccgg taaacctgga gaccgccctc aggatgtccg atccattggg agggcatctg
      661 gtttccggtc acgtggacgg gacgggggag atcctggagg tcgaacccga ggggaattcc
      721 tggcgctacc gctttcgggt cccccgggag atcagccggt acctggtgga aaagggatcg
      781 gttgcagtgg atggggtcag cctgacggtg gtggagtgcg gagatgagga gttttcggta
      841 agcttgatcc ctcatacggc ccaatccacg accctgggga ggaaaagagc cggggacctg
      901 gtcaacctgg aaaacgacat cattgccaaa tatgtggaga agtttttacg ccagaaggag
      961 gacccggcac agcagccctc tcgaatcgat gcctcctttc tggcccgaca cggttttaag
     1021 gagtcgatgg agtaagaacc atggccgtca gccgcatcga ggaagcactg ctggatatcc
     1081 gcaaggggaa aatggtcatc ctggtggatg acgaagaccg ggagaatgag ggcgatctct
     1141 gcatggccgc ggagaaagtg aatccaccgg ccgtcaactt tatggccaag tatggacggg
     1201 gtctgatctg cctttccctg caggaggaaa aggccgatga actgcagctt cctcccatgg
     1261 tctctaacaa tacctccagc tttgggaccg ctttcacggt atccatagaa gccaagcggg
     1321 gcgtcaccac tggcatttcg gccgccgacc gggcaactac gatcctcacg gccattgccg
     1381 acgaagctaa gtcggatgac ctggtgcgcc cgggacacat ttttcctcta cgggccaaaa
     1441 acgggggggt tctcgtccgc acgggccaga ccgaggggtc cgtggacttg gcgcggctgg
     1501 ccgggctgaa gcctgccggg gtcatctgcg aggtcatgaa cgatgacggc accatggccc
     1561 gcatgcccga cttggaggtt ttcgccaaaa agcacaacct gaagatcgtg accatcgccg
     1621 acctgatcag ataccggctt cagaatgaat gcatggtgcg cagggcggcg atcaccaccc
     1681 tgcccaccgt ttacggcgga gagttcaccg cgatcgccta cgaaaatgat gtggaccacc
     1741 agcagcacat ggcgctggtc aagggggaga tccgtccgga ggagcccgtg ctggttcggg
     1801 tccattccga gtgcctcacc ggggatgtct tcggctccca gcggtgcgac tgcggggaac
     1861 agcttcacac cgccatgcag atcgtggcta aagagggaat gggagtcatc gtttatatgc
     1921 accaggaggg gcgggggatt gggttggcca acaagatcaa ggcctacgcc cttcaggacc
     1981 agggaaagga caccgtggag gcgaatgaag ccctgggctt tgcccccgat ttgcgggact
     2041 atgggctggg agcccaaatc ctgcgggacc tgggggtccg caagattcgc ctcatgacca
     2101 acaatcccaa gaaaatcgtg ggcctccagg ggtatgggct ggaagtggtc gatcgagttc
     2161 ccctggaaat caaacctggg cggcggaact ttggttattt aaggaccaag aagaccaagc
     2221 tcggccactt gctgaccctg gagaaagagg gagggacgaa aaatgccaaa aacgactgaa
     2281 ggaaagctca atgctcaagg actcaggttc gcaatcgtag tcagccgttt taatgatttt
     2341 atctgcgagc atcttctctc cggggctctg gatgttttga tccggaatgg ggcggcagac
     2401 ggggatatcg aggtctttaa agttcccggg gcctttgaaa tcccccaggt ggccaagaag
     2461 gtggtccttt cgagaaaatt tgatgcggtt atctgtctgg gtgcggtcat tcgaggggcg
     2521 actccccatt tcgaatacat cgccgccgaa gtttccaagg gtgtggccat gatcggattg
     2581 gagggcgaag tcccggtcgc tttcggagtt ctgaccacgg ataatctgga gcaagccatc
     2641 gaaagggcag gaaccaagtc gggaaacaag ggctgggatg cggccctctc ggccatcgaa
     2701 atggccaact tgtaccggga aatgaagggg aaaaaataaa aaacattcac cacagaggcg
     2761 cagagttcgc agagaaagaa taaataaaaa acgaccccaa attttttctt tgtgttctct
     2821 gtgtctccgt ggtgaaaaag gtctttgaat gacgattcgc aggagggcga gagagatcgc
     2881 gctccaggtt ctttaccaac tagatatcgt cccgggagac ccgcaagagg ctctgaccct
     2941 gtactttgaa aactttcacc cttccgagaa aacccgggaa ttctgccggc ggctggtcga
     3001 aggggtctgc cagggacaag gagaaatcga ccggctcatc gaagagaatg ccgaaaactg
     3061 gactttgaag agaatggcgg tggtagatcg aaacatcctc cgcttggcca cttttgagct
     3121 tctcaactgc ccggatatcc ccttcaaggc gagcctcaac gaggccatcg aattggccaa
     3181 gaagttcggc aacgacgact ccagcgcgtt catcaacgga atcctagacc aaatccgttc
     3241 tctgggaggt gccaatctcc cagacgggac accgctttcc aagaagccaa catgaggtgg
     3301 gcaatccttt ctgatgttca cggaaacctg gagtccttcc gggcggtgat cgaggacctt
     3361 cggggggaaa aggtggagag ggttgcctat ctgggagacg tggtgggtta tggggcagac
     3421 cctaatgatt gcctgacctt gctggagggg ttaaccgatt gggtagtggc cgggaatcat
     3481 gattacggtt ccatcgggtt gaccgacatc gatgttttca acccctcggc ccgctccgcc
     3541 actctttgga cgagggacca acttctcccc gaaaaccgga tttatctccg gaatcttctc
     3601 ctcaggcgcc agattggaga gatcaccttc gtccacgcca ccccgaacca gccggaagat
     3661 tggaattata tatttacctt ccccgaggca gaagaggcct tccagtccct gaccggaggt
     3721 ctggccttta taggccactc ccaccggccg ttaatattgg ccaaggaaaa ggattcggaa
     3781 gtgcgggtca tcgaccacga agaagtgatc ctggaatccg ggattcgcta catcattaat
     3841 gtgggaagcg tgggacagcc ccgggacggt catcccgagg ccgcctatgg gctttatgat
     3901 gagacgggga agacctatcg actaaagcgg gtggactacg atgtggccac cgcccagaaa
     3961 aagatcatca aagccggcct tccctccttc ctcgcccagc gcctatcccg aggaatctaa
     4021 taaggacaat ttcgagtttc gggtctcaag tttcgggttt gggctttgct tttcgggttt
     4081 gtctttttct tactaatgag gccataataa tctggacagg gagtttattt cttcctgatc
     4141 ctggattccg gcttccgccg gaatgacaac aaagaaccgt gccattcaat gtccggcact
     4201 ccggcgaaag ccggagtcca gttcaagatt ttttgtagga gagaattcct accaatgatg
     4261 tccagacaat aatggactcc ttaataactt gaaactcgaa acctgtaact atttcttggg
     4321 gcgggtagcc catcgcatcc agctggttaa ttccatcgcc tggctggggc agagttcata
     4381 acagcaatag caggagatgc attttttctc atccaggaaa gggaatccct ggagagacag
     4441 tgcctgggcc ggacagtgct ggatgcagac ttcgcaccgg gtgcacgagt cccggcgcac
     4501 cttcagtctg ggtttgacga ggggacgata caggagctta ttgagcacgg ttccgaacca
     4561 tccccggctt gcgaagggga tgggggtctt gaattttttc agggattccc atgaaccctg
     4621 gatttccatt ctggccgggt ctatctctcc caatcccctc tgacctgcga cggccagatg
     4681 atccacatcc tgggctggaa tccccatcat agccgcaatc agtccgtcga tcaaaacccc
     4741 gttccgcgag gccaggactt ttccgataaa gcgaagctct ttgccggaag gaccattccc
     4801 ctccataccc actactgcat ccatgatggt caggtccgga acccggacct ggtagatgtc
     4861 caccaaggcc tcggaaaatt gcctgggccg gggaacggcg aagtgaacgc gggctttctc
     4921 ggctcccgcc agaatcccga acatgttctt gaccgccccc gtgatccggg tttggacatg
     4981 ggttttgaat ttgggaacat tgatgagcag gtctgcttcc aaaacgggcc tggagatgac
     5041 cactttcttt aggaatggag atttcagggg gacctgaacg gtctccctgg cgaaattgac
     5101 atatccgccg tcggccgctt ccagaatccc ggagacccgg gcgcaccgct cgttggccgc
     5161 gtatccactc attcccggat tgtcccccac ccagcaggtg gccccccttt tcttcaatgc
     5221 cttcaccaaa gatcgaatca gggatggatg ggtggtgatg cccttttcct gcggataggg
     5281 cccaagcatg ttcggtttta ggagaatctt tttcccgcta cagggaaggg gaaaagtctc
     5341 caaaacctct tccaccaatg cgtccatacc aggataatcc ccgtccaaaa ccaaaatcga
     5401 gggtcggacc ggcggtgggg ggtaagaaat catcatctca tcatccggag cggggaggag
     5461 accagaggaa caggattcat tcgaggacgg aggtgaggga aatctcgagt gccttctcgc
     5521 accaccgggc tagggccaat aatttcccct cggaaaaagc cttgccgacc aattgaatcc
     5581 ccaggggtag cccttcctgg ctcaaaccgg atgggagggt gatcgtcgga agcccggaga
     5641 aactccaggg gacctgaaac caggcatccc ccgtggattc gagacctcgg ggagctggag
     5701 aagaagtgga aggggtcagc agcaggtcgt atcgctccat gacccggtcc atctggcgcc
     5761 gaaactctct cttgattcgg taggcggtca gataatccac gccggggatc aaactcccga
     5821 tctcgatgag ctcccgcagc ttgggacggt acagctcccg gtgcttttcg taaagcggtt
     5881 ggtgaaacgc cgcgccctcg gtccgcatca cgatccggtg ggcgtcatgg accaccgcga
     5941 agcttcccgg catcttcacc gtctccgctt tggctcccgt ctttttgagt cgggccaaaa
     6001 cctcatccgt atttttccat acctgggttt cggaattttt ccgatagaac tcctccacca
     6061 ccccgatgaa gggagcccgg gaggattttg aggcccgggt ataatcgggg accggctccc
     6121 ggctggtggt aggatcctgg gtatcataac cggccagcac attgagaagg agggccgcat
     6181 cagataccgt tcgggtgaag aggcccacat gatccaggga ccagctgaag gggagaaccc
     6241 cgtatcggct aatccggccg taagtcggct tgagtccgac cgcgccgcaa taagaggccg
     6301 ggcggaggac ggagcccccg gtctgagacc ccagggcgcc gggacacatt cccgtggcca
     6361 ccgcggccgc agaaccgctg ctggacccgc cgggggtgtg ttcccggttc cagggattgc
     6421 gagtcggcgc cgggtcggca tgggcgaatt ccgtggtcgc cgtctttccc agtacgatgg
     6481 ccccggcctg cttgagccgc aggacggcag tggaatcaaa aaaggggaca aaatcggcta
     6541 ggaccttcga cccgcccgtg gtcttcaccc ccgccgtata gaaaatgtct ttaaccccca
     6601 ggggaattcc atggagcatg ccacgttttc ttcctcgggc ggtttccttt tcacaacgcc
     6661 gggcttcttc cagtaccttc tggcggtcca gggtcaccca ggcctggatg gaaggctcca
     6721 gggcatcgat gcgtttcagc agcgattcag ccagggccac cggagaaagt ttcctccgac
     6781 gaatttgctg gatcatttcg gttaaggtga gacggtacgg ctctgtcatt tccacctccg
     6841 atccaaaagc atagggcata gagcatagag aaagaaagat acgccatgcg ctatgcccta
     6901 tgctcttggc ctttttcacc gtgcgaaaga aggttcttcc tccatctcca gagggtaatt
     6961 ctcgatcacg acgagggccc g
//
LOCUS       DASPRR010000023         4630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_971882, whole genome shotgun sequence.
ACCESSION   DASPRR010000023 DASPRR010000000
VERSION     DASPRR010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971882"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..650
                     /locus_tag="VEL68_00845"
     CDS             393..650
                     /locus_tag="VEL68_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HXZ34544.1"
                     /translation="MPEQKIGEVIKFFSKPSVAAIKVTDGMLNVGDRIKFKGHTTDFE
                     DQVQSMQVDNQPVEKAEPGALIGIKVKDRVREKDLVYKITD"
     gene            736..1548
                     /locus_tag="VEL68_00850"
     CDS             736..1548
                     /locus_tag="VEL68_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ34545.1"
                     /translation="MEGVSLQVAAGEFLGIVGKTGSGKTTLAQHFNGLLKPSAGNVYV
                     EGRNLADPGISWPEIRRRVGLVFQYPEHQLFAETVFEDIAFVLDQQKSFSPEEIKERV
                     RHVCGMVGLDYDSFGQRSPFALSGGEMRRAALAGVLVQDPPILILDEPTVGLDGPGKR
                     EVLRQIGNLHRLGKTIVIVSHAVEDLAEQADRLMMMEEGRIIASGEPREVFAQLVGMK
                     KFNFLVPPVFHLCEDLRAAGWTLPDKVFQTEEALSAIDRNLRAGHHPPEMEN"
     gene            2198..3037
                     /gene="accD"
                     /locus_tag="VEL68_00855"
     CDS             2198..3037
                     /gene="accD"
                     /locus_tag="VEL68_00855"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXZ34546.1"
                     /translation="MGDCFQRFMIGEYQGPQWTKCDACQQMLYRREVERNLMVCPKCN
                     YHFRISAAYRLNLVIDEGSFEEFDGDLEPMDPLHFKDRMKYSERVKESQQSTRRKEAM
                     VGGKGAIEGQPLLMGVMDFEFMGGSMGSVVGEKVARLAERAMEEGYPLVMFCSSGGAR
                     MQEGILSLMQMAKTSAALARLKEKGLPYISVLTDPTTGGVSASFAMLGDVILAEPKAI
                     IGFAGQRVIEQTIKQKLPEGFQRSEYLLEHGMIDLIVERREMKKVLGRLLALLPKNKK
                     PAG"
     gene            3046..4419
                     /locus_tag="VEL68_00860"
     CDS             3046..4419
                     /locus_tag="VEL68_00860"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HXZ34547.1"
                     /translation="MQKDGEEKRDYRESLDYLFGLQRFGIKLGLSNIIALLKHLGNPH
                     QGLRAVHIAGTNGKGSTAAFLTSILAQAGCRVGLYTSPHLIDFTERIQVDGIPLPEGR
                     VAKLTDRIREVVREMERSGELWADSSAFPPAKDFDPQKATITFFEFTTAMAFLHFREE
                     QVDVAVLETGMGGRLDATNAIDPLLSLITPVSLEHQQYLGRTLLQIAGEKAGIIKPGR
                     PLLSTARQPRVIDFFRRKCLELGAPFYAHGKEFQGRQIEPRLMDFQGIGHRWDALCLG
                     LAGGHQVLNASLALAAAEILMEKGFPIREDSVRKGVAGTQWPGRLELVGDSPRVLLDG
                     AHNPEASRVLKQALQEEFPRRRLILVLGIMADKDIPKMMSNLIPLADLLILSRPHMDR
                     AASLETLRKNAFTWGKPTQEVADVGTAVEKAMGKAGKEDLVVVTGSLFTVGEARAHLI
                     RKGFIQR"
BASE COUNT         1002 a         1251 c         1353 g         1024 t
ORIGIN      
        1 ggaaccgtcg tgcgagaccc gaaatcggaa gctgaaccct gaaattcgtc gagcccctcc
       61 tccaagccag ttttttttga ggcaaatttt ctgcaacgcc ggagggcgta ttaagtttga
      121 cctttccggt atgcctgggt cgtggcctgg aggcaaggag cttctcagta ataagcatcg
      181 gcccattgaa ttttggattt tgaattgcgg atttcggatt tttcggtttt tctatttcaa
      241 tccgctttcc gcaatccaca ttccgcaatt tcagaggtcc gccaggtcca tgaaagcacc
      301 cattttatgc cttgacactc cggaaagcga aaacctataa tttttttatc aaggctccca
      361 agcgatcaga agcccggagg aaggaggaac agatgccgga acagaagatc ggtgaagtga
      421 tcaagttttt ttccaagccc agcgtggcgg ccatcaaggt gaccgacggc atgctcaacg
      481 tgggcgatcg gatcaagttc aaggggcata ccacggattt tgaagaccag gtccagtcca
      541 tgcaggtgga taatcaaccc gtggaaaagg ccgaaccggg cgcgctgatc gggatcaagg
      601 taaaagatcg ggtgcgggag aaggacttgg tctacaagat cacggactga ggaaaaaccc
      661 atttctgcgc cgcccctgtc ggttgagaac ctgacccacg tctaccatcc ggggactccc
      721 ctggagaaaa gggccttgga gggcgtatcc ctccaggttg cggcggggga atttttgggg
      781 atcgtgggga agacgggctc gggaaagaca accctggcgc agcattttaa cggcctgctt
      841 aaaccctccg cgggaaacgt ctatgtggag ggaagaaacc tggcagaccc cggaatttcc
      901 tggccggaaa ttcgccggcg ggttggcttg gtcttccagt atcccgagca tcagcttttt
      961 gctgagaccg tctttgagga tatagccttt gttctcgacc aacagaaatc cttttccccg
     1021 gaggagatca aagaacgggt tagacacgtc tgtggcatgg ttggcctcga ctacgattct
     1081 ttcggccagc ggtctccttt cgcgttgagc ggcggggaaa tgcgccgggc cgccctagcc
     1141 ggggttctgg tccaagaccc gcccatcctg atcctcgacg agccgacagt ggggctggat
     1201 ggtccgggca agagagaagt cctgcggcaa atcgggaatc tccatcggct ggggaagacc
     1261 attgtcatcg tttcccacgc ggtggaggac cttgccgagc aggcggatcg attgatgatg
     1321 atggaggaag ggaggatcat agcaagcggc gagccaagag aagtcttcgc ccagctcgtg
     1381 gggatgaaga aattcaactt tctggttccc ccggtctttc acctgtgcga ggatctccgg
     1441 gccgcaggat ggacccttcc ggacaaggtc ttccagaccg aggaggcgct ttcggcgatc
     1501 gaccggaatt tgagggcggg ccaccacccc cccgagatgg aaaattagtc ctccgcgact
     1561 cgcctctttc tccatgggcc tcctgcccgt agccctttta ttgcctcaaa gagaaaactt
     1621 tcggctctgc cgaagggccc ataaagtttg acctttccgt catgcctggc acccggtcga
     1681 aggctgccct ccggcggccc ccggttgaat gcgctttgct aggttccctg tttatcaagc
     1741 ctccggctct gccgcaggct attccttccg aggcccgaat cgatccgtcc gccttctccc
     1801 cctgcatttc cgcctgactg gggaggagat tcccgcttga tcgaggatga tccggtcacc
     1861 gccgatgacc acgccctttt gaatctccct gcacgcaaga cccttttgag tacacagcgg
     1921 aagccaactc caaggatttt tcctgaatct cacggggcag cagttccctg cctgggaccg
     1981 gaatcggagg aggtgtaaga atcaggcgag ttattccggg gctagaggag gcagaggccc
     2041 gcaaggtggg acggaggatg aaataatgat tgataactat ttgaacttat gataatttct
     2101 aatcttcaca tttcatcgac cagggattct tgaggggcgt aaaaggattg cccatagaga
     2161 ttcttgttat ctccatcttc cagtgagggc aacctggatg ggggattgct ttcagcgttt
     2221 tatgattggg gaatatcagg gtcctcagtg gacaaaatgt gacgcctgtc agcagatgct
     2281 ctaccggagg gaagtggagc gcaacctaat ggtctgcccg aaatgcaatt atcatttccg
     2341 aatttccgcc gcctatcgcc tcaatttggt gatcgatgaa ggaagttttg aggagttcga
     2401 tggggacctc gagcccatgg accctctgca ctttaaggac cggatgaaat actccgaacg
     2461 ggtcaaggaa agccaacaat ccacccggag aaaggaagcc atggtcgggg gaaagggagc
     2521 catcgagggg cagcccctgc tgatgggagt catggacttt gaattcatgg ggggctccat
     2581 gggatcggtg gtgggcgaaa aagtggcccg cctagccgag cgggcaatgg aggaagggta
     2641 ccccttggtt atgttctgca gctccggagg agcccgcatg caagagggga ttctgtccct
     2701 tatgcaaatg gccaaaacca gcgctgcctt ggccaggctg aaagaaaagg gtctgcctta
     2761 tatttcggtc ctcaccgacc caaccacggg cggggtgtcc gcaagcttcg ccatgctggg
     2821 ggatgtgatc ctggccgaac ccaaggccat cattggcttt gccgggcagc gggtcatcga
     2881 gcagacgatc aaacagaaat tgccggaagg cttccagcgg tccgagtacc ttttggaaca
     2941 cgggatgatc gacttgatcg tcgaacggag agagatgaaa aaagttctgg gccgactcct
     3001 ggccctactg ccgaagaaca agaaaccggc ggggtaggac gaaaggtgca aaaggacggc
     3061 gaagagaaga gagattaccg ggagtccctg gattatcttt tcggtttgca gagattcggc
     3121 atcaagctgg gcctctccaa tattatcgcc cttctgaaac acctgggaaa cccccatcag
     3181 ggtctgcggg cggtccacat cgccggtacc aacgggaaag ggtccaccgc tgcttttttg
     3241 acttccatcc tggcccaggc cgggtgccgc gtcggccttt acacctctcc tcacttgatc
     3301 gacttcaccg aacggattca agtagacgga atcccgttgc ccgagggacg ggtggccaag
     3361 ctcaccgacc gaatccgaga ggtggtcagg gagatggaaa ggagcggcga actctgggcc
     3421 gattcatcgg cctttcctcc ggctaaggat ttcgacccgc aaaaggcgac gatcactttt
     3481 tttgaattca ccacggccat ggccttcctc catttccggg aagagcaggt ggatgtggct
     3541 gtcctggaaa cagggatggg gggccggctg gatgcaacca acgccatcga tcccctcctt
     3601 tcgctcatca ctcccgtctc ccttgagcat cagcaatacc tgggaagaac ccttctacag
     3661 atcgctgggg agaaggcggg gatcatcaaa cccgggcgcc cccttcttag cacggcccgc
     3721 cagccccggg tgattgactt cttccggcgg aaatgcctgg agctgggggc gcctttttat
     3781 gcccacggaa aggagtttca gggaaggcag attgagccgc ggctcatgga ttttcagggg
     3841 atcggtcatc gctgggacgc tctttgcctc ggcttggccg ggggccacca ggtgctcaac
     3901 gcctctctgg cccttgccgc cgcagaaatc ctcatggaga aagggtttcc catacgtgag
     3961 gactccgtga ggaaaggagt cgcggggacc cagtggccgg gccggctgga actggtgggg
     4021 gattctcccc gggtccttct ggacggtgct cacaacccgg aagcgtcccg ggtcctgaaa
     4081 caggcgctgc aggaggaatt cccccgcaga cggctgatcc tggttctggg gatcatggcc
     4141 gacaaagaca tccccaagat gatgtccaac ctgattcctt tggctgatct gctgattttg
     4201 tcccgacctc acatggaccg ggctgcctcc ctggaaaccc tgcgaaagaa cgccttcact
     4261 tggggaaaac cgacccagga agtggccgat gtgggaaccg ccgtcgaaaa ggccatgggc
     4321 aaagccggga aggaggacct ggtagtggtg accggctctc tcttcaccgt cggagaggcc
     4381 cgcgcccatt tgatacgcaa ggggttcatt caaaggtaac ccggacctga tccgcgtttt
     4441 ctctttcacc cccgaccgaa attttattgt ttaatatttc ttttctatgc gctatgcttt
     4501 ttgcaaaatg cggtttctga tgattctttg ctttgccctg tgcgcccctc ttatttttct
     4561 ttatgcccta tgttctatgt gctatgcgct ttttctattc gttggtagga ggctcggatt
     4621 tcaaaccggg
//
LOCUS       DASPRR010000024         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_811824, whole genome shotgun sequence.
ACCESSION   DASPRR010000024 DASPRR010000000
VERSION     DASPRR010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811824"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VEL68_00865"
     CDS             complement(<1..431)
                     /locus_tag="VEL68_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXZ34548.1"
                     /translation="MNSIRRKVYCQIKHRRFGWIPLVFAVFTLGLIFSGSVLGAEKRP
                     NILFILSDDHRWDAMGNMGHPFIQTPSLDRLAKEGVRFNNAFVTTPLCSPSRASFLTG
                     QYPHRHGVKNNLTPWNDRNITFLELLKKAGYKNAFIGKWHMP"
     gene            477..1115
                     /locus_tag="VEL68_00870"
     CDS             477..1115
                     /locus_tag="VEL68_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HXZ34549.1"
                     /translation="MYPTLEFAAISLSLIFFIVDPLGNIPAFLAITEGRSADERKRTA
                     FRASLVTFLILLVFAFVGEWVLKLLRVTISSFQIAGGILIFIIALSMLQARRSRAKST
                     PQEEHEKHEGEDVAIFPLAIPMLSGPAAITTVMVLINLSTSTLQRGLVILAIFLTSLF
                     SYWIMAVSGQLMKGLGQTGINVLTRLMGLLLAVISVQFVIDGIKAAFLQTPP"
     gene            complement(1179..2351)
                     /locus_tag="VEL68_00875"
     CDS             complement(1179..2351)
                     /locus_tag="VEL68_00875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXZ34550.1"
                     /translation="MSSRWLSERIKGIGYSKIREVMGRVEEAKRKGIKITDLSIGRPD
                     FDTPGHIKEAAAKALADGLVHYTASAGTLELREALCRRFQEDHQLKIDPAEIIVTTGA
                     AEAIFIGLMSVLDPGDEVLVPDPMYVYYGGYVFMGGAKCVPIPLREEEQFLLKAQDLE
                     KYVTEKTKVLILNFPHNPTGQVLLQEDLEPIARVANRHNILVVADDIYNAMLYDEAKF
                     FPLAKAPGMKERTLTIGSFSKTYAMDGWRIGYLIAPREVISHALKLHQHVVSCPNTFV
                     QVGAQAALTASQDCVRKMVAEFDRRRRRLMSRLGEIGLPYVRPRGAFYVFPSIKKFGL
                     TSNQFADFLFQKARIAVVPGDAFGPGGEGYVRISYCAPVEEIEEAMDRVRSALATL"
BASE COUNT          502 a          681 c          632 g          584 t
ORIGIN      
        1 ggcatatgcc acttgcctat aaaggcattt ttgtacccgg ccttcttcaa gagttcgagg
       61 aaagtgatgt tccgatcatt ccacggggtg agattattct tgaccccgtg ccggtgcggg
      121 tactgcccgg taagaaaact cgcccgcgaa gggctgcaaa ggggggtggt cacaaaggcg
      181 ttgttgaagc ggactccctc tttggccaac cgatcgaggc tcggggtttg gatgaaggga
      241 tggcccatgt tgcccatggc atcccagcgg tgatcgtcag aaaggatgaa gaggatgttg
      301 ggtctcttct ccgccccgag aaccgacccg gaaaagatca ggcctaaggt gaatacggca
      361 aaaaccagtg gaatccatcc gaaccgtcgg tgcttgattt gacaataaac ttttcgtcga
      421 atagaattca atacgagttc tgctcctcaa ctccgttttt tcggacggac cttattatgt
      481 acccgacctt ggaatttgcg gccatctccc tgtctttaat cttcttcatt gtggacccac
      541 tgggaaatat ccctgctttc ctggccatca ccgaagggag gagcgccgat gagagaaagc
      601 ggaccgcctt ccgggccagc ctggtcacct ttctgattct gctggttttc gctttcgtgg
      661 gcgaatgggt cttgaagctg cttcgggtaa cgatcagctc gtttcagatc gccggcggaa
      721 tcctgatctt catcatcgcc ctcagcatgc ttcaggcccg tcggtccagg gccaagtcca
      781 ctccccagga ggagcatgag aagcatgaag gggaagacgt ggccatcttt cctctggcca
      841 tccccatgct cagcggcccg gcggctatca ccacggtgat ggtgttgatc aacctgtcga
      901 cctccacgct tcagagagga ctggtcatcc tggctatctt cctgaccagc cttttctcct
      961 attggatcat ggcggtatcc gggcagttga tgaaggggct cggccagaca ggcatcaacg
     1021 tccttacccg cctcatggga ctccttctgg cggtgatttc ggtgcagttt gttatcgacg
     1081 gaatcaaggc tgctttcctg caaacacccc cttgattgga aagagaccgg ggcccggtca
     1141 gtatttggag ctcaacggcc tccccatccc ttcttttttc aaagcgtggc taaagccgag
     1201 cgaaccctat ccatggcttc ttcgatctct tccacagggg cacagtagga tatccgaaca
     1261 tatccttccc ctccggggcc aaaggcatct cctggcacca ctgcgatcct ggctttttgg
     1321 aacaggaaat ccgcaaattg gttggaggta agcccgaatt tcttgatcga aggaaagacg
     1381 taaaaggccc cccgggggcg cacgtagggt aagccgatct cacccagccg ggacatcaac
     1441 ctcctgcgtc gccggtcgaa ttcggccacc atcttccgga cgcaatcctg ggaggcggtc
     1501 aaggccgctt gagcccccac ttggacaaag gtattcgggc agctgaccac gtgctgatgc
     1561 aatttcagcg catgggaaat cacttcgcga ggagcgatga gataaccgat acgccagccg
     1621 tccatggcgt aagtctttga gaagctgccg atggtcagtg tcctctcctt cattccggga
     1681 gctttcgcca ggggaaaaaa ttttgcctcg tcatagagca tggcattgta aatatcgtca
     1741 gccaccacca ggatattatg acggttcgcc actcgggcga tcggctccag gtcttcctga
     1801 aggagaactt gtcccgtcgg gttgtggggg aaattaagga tcaacacttt ggttttttcg
     1861 gtgacatact tttccaggtc ctgggccttc agcaagaact gctcttcttc tctcagggga
     1921 atgggaacgc acttcgctcc ccccataaaa acatagccgc cgtagtagac atacatgggg
     1981 tctggcacca ggacctcatc gcccgggtcc agaacgctca tgagtccgat aaaaatcgct
     2041 tccgctgccc ctgtcgtgac gatgatctcc gctgggtcaa tcttcagctg atgatcttct
     2101 tgaaaacggc ggcagagggc ctctcgaagt tccaaagtgc cggcgctggc cgtgtagtgg
     2161 accaacccat ccgccagggc ctttgccgcc gcttccttga tatgtccggg ggtgtcaaaa
     2221 tcgggcctgc cgatggaaag atcggtgatc ttgatcccct ttcgtttggc ctcttccacc
     2281 cttcccatca cttctcttat ttttgagtac cctatgcctt taattctctc tgacaaccaa
     2341 cggcttgaca tcttttcctc ctcctaattt ctgaaagcct gcgagggctt ttcacggga
//
LOCUS       DASPRR010000025         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1136077, whole genome shotgun sequence.
ACCESSION   DASPRR010000025 DASPRR010000000
VERSION     DASPRR010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136077"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VEL68_00880"
     CDS             complement(<1..288)
                     /locus_tag="VEL68_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ34551.1"
                     /translation="MLNPGECKKTGINWIEANSSRLIDFSKQIWNYAELGLEEHRSFR
                     VLADFLSIEGFTLEKDVAGIPTAFVAAWGQGKPVIGINCEYDALPGLSQEPT"
     gene            complement(299..1273)
                     /locus_tag="VEL68_00885"
     CDS             complement(299..1273)
                     /locus_tag="VEL68_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34552.1"
                     /translation="MKIGKLFSSTKNKIITVIVIVLLLLPIFVEDPYIMNIIILSLLF
                     AVLACSWNLICGYTGIFTFGHQAFFGLGAYVSSLLAMKAGLSPWLGLLAGGFAAALVG
                     FVIGLPCLRLRAAPYIAITTLAFSEIARIVCMNLVGLTRGELGLWGIPEFPEIPLPGG
                     LAVAFAGGDRTGYYYVILMIFFVTIGLIAWMLRSYVGLAFRAVRDEQDAALSLGIDTT
                     RFKLLAFITSSFFAGVAGAFYAHYILILTPTSVMSVGVMTEIIAVTLVGGLGTFWGPV
                     IGAFSLTALLEYLREFGEYRFMIYGAMLVATIMFMPKGVSSKLFPEKE"
     gene            complement(1418..2293)
                     /locus_tag="VEL68_00890"
     CDS             complement(1418..2293)
                     /locus_tag="VEL68_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34553.1"
                     /translation="MNLQLIVEQMLNGMVLGSMYALVASGLTLIWGTMKMLNFAHGEL
                     YMIGGYVLYFAFLLFGAHPFWGILLAMLIAFALGVLMERSILRPLLDKPGWDVSPIVA
                     TLGVSIFLQNFALKVWGERFKSVPYFIEGTLEFLGFRIAYQRILILVAAAAVMIGFWI
                     YVKRSRFGMALRATAQDREAATILGINSHRIYLMTFGISSALAALAATMLAPLFLVNP
                     WMGIPPQLKAFIVVVLGGLGSFGGAILGGFLLGSIESLAIVFLSSEWKDVAAFLVLIG
                     FLVFKPAGFFGTKEW"
     gene            complement(2359..>2574)
                     /locus_tag="VEL68_00895"
     CDS             complement(2359..>2574)
                     /locus_tag="VEL68_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34554.1"
                     /translation="GLAPVIVERIFEVIERINQKGVTVLLVEQNAHIALQIAHRGYVI
                     ETGHVVMEGEANRLSQDEYIKQAYLGL"
BASE COUNT          661 a          724 c          673 g          516 t
ORIGIN      
        1 ggtcggttct tgggaaaggc cgggcagagc atcatactca caattgatcc cgatgaccgg
       61 ctttccctgg ccccaggcag ctacaaaggc cgtggggatc ccggccacat ccttttccag
      121 ggtgaagccc tctatggaaa gaaaatctgc caggacccga aaagatcggt gttcttccag
      181 acccaattcg gcgtaattcc agatttgctt cgaaaaatcg atcagcctgg agctatttgc
      241 ctcgatccaa ttaattcccg ttttcttgca ttctcccgga ttaagcatgg actctccttt
      301 attctttttc cggaaataac ttgctggata cccctttggg catgaacata atcgttgcca
      361 ctagcatggc cccgtagatc atgaagcggt attccccgaa ctcccggagg tactccagaa
      421 gagcagttaa ggagaaagcc ccgatgaccg gaccccagaa ggttcccagc cctcccacca
      481 aggtgacagc gatgatctcg gtcatgaccc ccacgctcat gaccgacgtg ggggtcagga
      541 tcagaatgta gtgggcgtag aaggctccgg ccacgccggc gaaaaaggaa ctggtgataa
      601 aggccagcag cttgaagcgg gtggtgtcga tccccaggct gagggccgcg tcctgttcgt
      661 cgcgcaccgc ccggaaggcc agccccacgt aggaacgcag catccaggcg atcaacccga
      721 tggtaacaaa gaagatcatc aggattacgt aataatagcc ggtccggtcc cctcccgcga
      781 aagcgactgc cagtccgccg ggcaggggaa tttccggaaa ctccggaatt ccccagaggc
      841 ccaattcccc ccgggtcagg ccgaccaggt tcatgcagac gatccgggcg atctcggaaa
      901 aggccagagt agtgatggca atataaggcg ccgcgcggag gcgcaggcag gggagaccga
      961 tcacaaatcc caccagggct gcagcgaatc ccccggccaa gagccccagc cagggcgaga
     1021 ggcccgcttt catagccagg agagaggaaa cataggctcc taagccaaag aaggcctggt
     1081 gaccgaaggt gaagatcccc gtataaccgc agataagatt ccagctgcag gccaaaaccg
     1141 cgaaaagcaa tgacagaatg atgatgttca tgatgtaggg gtcttcgacg aagatcggaa
     1201 ggagcagcaa aacgatgact atgacggtaa tgattttgtt tttggtggag gaaaagagtt
     1261 ttccaatctt catttcccga tccctcagct taccattcag acaaataaca agcagataag
     1321 attaagcaag gtaattgaat tcatggcttg aaaccctgta ttttaaactt gaaaagtttt
     1381 ttctctgtgt cctctgtatc tctgtggtga aaaatattta ccattctttg gtgccgaaaa
     1441 agcccgcagg cttgaacacc aggaatccga tgagaaccag aaaagcggcc acgtctttcc
     1501 actctgagga gagaaagacg atggccaggc tttcgatgga acccagcagg aatcccccca
     1561 gaattgctcc cccgaaactc cccagccctc ccagaaccac cacgatgaag gccttcaact
     1621 gcggtggaat gcccatccag ggattgacca gaaagagagg agccagcatg gtggccgcca
     1681 gcgcagccag ggccgaactg atcccgaagg tcatgagata aatacggtgc gagttgattc
     1741 ccagaatggt ggcggcttcc cggtcctgag cggttgcccg aagggccatg ccaaagcggg
     1801 atcttttcac ataaatccag aaccctatca tgaccgcggc ggcggcgacc agaatcagaa
     1861 tccgctgata ggcgatccgg aatccgagaa attccagggt cccttcgatg aaatagggaa
     1921 cgcttttaaa ccgctccccc cagactttca gagcaaagtt ctggaggaag atggaaaccc
     1981 ccagagtcgc cacgatgggg ctcacatccc atcccggctt gtccagcagc gggcgcagga
     2041 tcgaacgttc catcaggact ccaagggcaa aggcaatcaa catggccagg aggattcccc
     2101 agaagggatg cgccccgaag agcaggaagg cgaaatagag gacatacccc ccgatcatgt
     2161 agagctctcc gtgggcgaag ttgagcattt tcatggtgcc ccagatgagg gtcagtccgc
     2221 tggccaccag ggcgtacata ctgcccaaca ccattccatt caacatctgt tcgacaatca
     2281 gctgcaggtt cataaaggat cttcttgctt cttaacttta ggcatttaag gcactttagc
     2341 gcactctagg cactttcttt ataatcccag ataggcctgt ttgatgtact catcctggct
     2401 cagtcggttg gcttcccctt ccatgaccac gtgccctgtc tcgatcacat atccccggtg
     2461 ggcaatctgc agagcgatgt gggcgttctg ctccacaagc agaaccgtca cccctttttg
     2521 gttgatccgc tcgatgactt cgaatatcct ctccacgata accggagcca gccc
//
LOCUS       DASPRR010000026        10339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2105409, whole genome shotgun sequence.
ACCESSION   DASPRR010000026 DASPRR010000000
VERSION     DASPRR010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105409"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..668
                     /locus_tag="VEL68_00900"
     CDS             105..668
                     /locus_tag="VEL68_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ34555.1"
                     /translation="METVHLNFPVRKPGNFKGRKVRVMPSPALMEQYRSYGATPVPID
                     LTELYNALQLGTVDAQENMLYRIEEMKYYEVQKYITISNHALMPEIVVVSKLWWDKLP
                     GDIQKKMISIFKEIAPHEAKWIDEDDARALKVFEKHGNILYNLTPAEWKEFQKLAPGV
                     WDKFVAEYGGKSGYYLDKLKAAIAGAQ"
     gene            838..1365
                     /locus_tag="VEL68_00905"
     CDS             838..1365
                     /locus_tag="VEL68_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ34556.1"
                     /translation="MERFFKFNQYVMLGGKVLVGAILTGAAFVLFVNVVLRYGFGYGI
                     SWAEEVTRYTMLWTVFIGAGVIAREGTHVSMEAFFNLWPEKARRIGFLAINIFCIVTI
                     MTILSFGMGIVRMVIETGQTSEAAFIPMWIIYGAFPVGSILMILGYAETALRHSVQKP
                     IQGSDISLDQGRHDS"
     gene            1365..2654
                     /locus_tag="VEL68_00910"
     CDS             1365..2654
                     /locus_tag="VEL68_00910"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ34557.1"
                     /translation="MLTTLCTLGGLLLLLNVPMFVLMVATSAVIIWAYDVAPLMLIAT
                     QMFGSIDKFALMAIPFFIFGASIMGAGGLTTRLIAWVEAMVGWVRGGLAYTTILTCEI
                     FGTVSGSSAATTAAVGSILYPSLVKRRYGEVFSLGLVTCTGAIASVIPPSIGMIVYSA
                     ITGASVGAVFMGGVGPGILLGICFAGWVAAHTRREKVPQMGSFDFRLVRQRTQSAGWA
                     LGMPLLILGGIYSGVFTPTEASAISTIYAMFVAFFIYKEMTLKKLFQIAIESGLITAK
                     IMIIIASSGVFVWILTVGGVPDMVNNWVQSVGASQFLVLLLINVVFLIAGMFLEPNST
                     LVVLTPLFYPIATRLGVDPVHLGVILVLNISIGMYTPPFGFNLFVSMSIFNAPMRKII
                     AGLTPFIIVSLLALFITTYVPQITLWLPKILYPKSFP"
     gene            complement(2728..3276)
                     /locus_tag="VEL68_00915"
     CDS             complement(2728..3276)
                     /locus_tag="VEL68_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXZ34558.1"
                     /translation="MDLYEAIEKRRTIRIFKKGATEEQLRKIILAGSKAPSGGNRQPW
                     EFIMIDDRKIIDQLAEIKYQLNRKMAPGAGEGQKEVEERALNQKKWFQNSSVVAVCTS
                     SGQATNGWLAVENMSLAAVAEGLGSNIISYWGEEKKEVEKILGLPADYELTCVLKFGV
                     PGEGGPVPKRRPEGSWLHKNKF"
     gene            3910..5667
                     /locus_tag="VEL68_00920"
     CDS             3910..5667
                     /locus_tag="VEL68_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HXZ34559.1"
                     /translation="MSESSEIRDGMRIDWDVPIAMDDGLELRADIYRPPQEGRYPVIL
                     SYGPYAKGLAFQEGYPDQWQRMADQHPDVTEGSTNKYQNWEVADPEKWVPDGYACVRV
                     DSRGCGRSPGYVDPFSPRETRDFYLCIEWAGTQPWSNGKVGLSGISYYAINQWHVASL
                     QPPHLAAMIPWEGAADWYRDMTYHGGIRCVFFGNWFKKQVMTVQYGLGRKGYTNPNTG
                     ELASGPETLSEEELLRNRTDLAAAIQDHPLDDRYHRERSPIWSKINVPFLSSANWGGQ
                     GLHPRGNFEGFTQAASKQKWLEAHGLEHWTEFYTRYGVRLQKRFFDHFLKGLDNDWKG
                     QPRVQLQVRRVDGFGQRSENEWPLARTRWTRFYLNPFDRSLSPKPVDAAGNLEYEALG
                     DGITFLSPPMEEETEITGPLAAKVFMSSSSKDADFFLVFRVFSPDGTEVVFQGALDPH
                     TPIAQGWLRASHRKLDPVLSKEYRPYHPHDEVEPLTPGKIYELDVEIWPTCIVVPKGY
                     RMALSIRGKDYEHPGKGARIKSFVNELKGCGPFLHNDPQDRPESLFGGKNTVYGGGKN
                     PSFLLVPIIPKRQVGASRG"
     gene            5966..6679
                     /locus_tag="VEL68_00925"
     CDS             5966..6679
                     /locus_tag="VEL68_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXZ34560.1"
                     /translation="MKPIRRVRLYENAVDQIQTLILSHEYKPGDRLPSERALAEHFHI
                     SRHSLREALRILDVMGLIEIRVGDGIYAKEVDFLPYIESLNLSISSRLPLERDSFVKL
                     WRARRILEAGIVDLAAKEVTESFLKSLWWCIGEMEKNITHQEAFISAGIRFHRLIAEV
                     AQNEILILIWDMLANLIRKSQSKIYGISWSPRKALSAHKKIYLALKARDPQKAVEAMK
                     QHMLDEEKALVAALGKGKG"
     gene            6752..8014
                     /locus_tag="VEL68_00930"
     CDS             6752..8014
                     /locus_tag="VEL68_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXZ34561.1"
                     /translation="MARDLSFIGRPHEYDARRVFSLTGADWQDRVNFDRLRKERLARA
                     RQQMEIHDLGALVVYDGANVRYLTSSFQGNWKYNIFIRYAVLCRGAEPILFETAGSDL
                     QCAKIDLPWMEGRIRPAITWKWSEGAVEYMVDRMVDSVVEVLKENKVEKEKIGIDSLD
                     MASLAAFQKKKINVVNAWPAMSAARVVKTQDEIELLKQSAAIGDACMYKARYEWAKPG
                     ITERELSGKMIEYMYSKGCEIVYEIIVASGGNTSPYRRWPTDKMIRQGDLIIIDNNTV
                     GPSGYFVDYVRCWKIEAKPTPKEKDLYKECYDSLMGAIEMMKPGKTSKDVAEKFPVYD
                     DDKYGTVTLQQFAHSIGLTLYEGMWISRAYSMKYPAEIKPNMYFAVETFAGHPLLEQT
                     VRLECDLVITDKGHELFTLFPFEEDMLK"
     gene            8146..8604
                     /locus_tag="VEL68_00935"
                     /pseudo
     CDS             8146..8604
                     /locus_tag="VEL68_00935"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
     gene            8687..9931
                     /locus_tag="VEL68_00940"
     CDS             8687..9931
                     /locus_tag="VEL68_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34562.1"
                     /translation="MGGRSIRTVIIGMVAGLILFTPLVSWGQAKGPIKIGFISPLSGG
                     MAANGKDMLAGIQLYLEEIGYQAAGRKIELIVEDDEANPATGLTKTRKLVEKDEVHVM
                     SGGLMASTGYALVPYIDSKEIPMTYPIMAPDDLTQRQRAKWIVRTGWNSSQPNHPLGE
                     YAYQVLKFRKVIIIGLDYAFGWECAGGFQKTFEDAGGKVIQKIWTPLTVTDFSPYLAQ
                     FSKEADGVYAMFTGRTTLQFTKQYQEFGLKGKLPLIGGGTTTDEHALPSMGDEAIGII
                     TSLHYSEALDNPANKKFVKAYREKAKKAASYYSEGTYTGARWIVEAIKNINGDVENRQ
                     KFMDALRKVELRDVPRGSFRLDDYGNPVQNVYIRKVERVGGELQNTVIHTIPNVSQFW
                     KYKPEEFLKQPVYSREFPPLKP"
     gene            9980..>10339
                     /locus_tag="VEL68_00945"
     CDS             9980..>10339
                     /locus_tag="VEL68_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34563.1"
                     /translation="MKKALFSILWMVAAVSLVLLNPSVSPAQGKGPIKIGYISPLSGG
                     MAANGKDMLAGIQLYLEEIGSQAAGRKIELIVEDDEANPATGLTKTRKLVEKDGVHLM
                     TGGLMASTAYALAPYIDS"
BASE COUNT         2330 a         2790 c         2947 g         2272 t
ORIGIN      
        1 aatattgata attactataa agtgatgacc gggcccttcg gggaggaatt gggccaggag
       61 tcccagaagg ccaagatggt gactctcgcc ttctggaccg ccggctggaa acagttcacc
      121 tcaacttccc tgtccgtaaa ccgggcaact tcaagggccg caaggttcgg gtcatgccgt
      181 cgccggcgct catggagcag tatcgttcct atggcgccac tccagtcccc atcgatctta
      241 cggaactcta caacgccctc caacttggaa cggtcgatgc ccaggaaaac atgctctacc
      301 gaatcgagga aatgaagtat tacgaagtgc aaaaatatat caccataagc aaccacgccc
      361 ttatgccgga gatcgtagtt gtcagcaaat tatggtggga taaactcccc ggggatatcc
      421 agaagaagat gatctcgata ttcaaagaaa ttgcgcctca tgaagcgaaa tggatcgatg
      481 aagatgatgc ccgagccctc aaggtctttg agaaacatgg aaacattctg tacaatctga
      541 ctcccgcgga gtggaaggaa ttccagaaac tcgcccccgg cgtatgggat aaattcgttg
      601 ccgagtacgg gggcaagtcc gggtattatt tggataagct gaaggcggca atcgctggcg
      661 cgcaataagg ctatggatga gaataagagc tgacggtctt gcgcccgctt gagggcgcat
      721 ttcttttttg ggcgggtaca ttggagattg tgtatcccgg acggaaccaa tggtatcggt
      781 ttcctgctct taaaagactc aaaaatatga gaaaggcccc ttcaaaaagg aagtgtcgtg
      841 gagagattct ttaaattcaa ccaatacgtg atgctcggcg ggaaggtcct agtgggcgcc
      901 atcctgaccg gcgccgcgtt tgtgcttttc gtaaacgtgg tattgaggta tgggttcggg
      961 tatggcattt cctgggctga ggaagtgacg cgctatacca tgctgtggac ggtttttatc
     1021 ggggctgggg taatcgctcg ggaaggcacc cacgtgagca tggaagcctt cttcaacctc
     1081 tggccggaga aggcccgaag gatcggcttt ctggccatca atatcttttg catcgttacc
     1141 attatgacga tattgtcttt cggtatggga atcgtcagaa tggtgattga aaccgggcag
     1201 accagcgaag ccgccttcat ccccatgtgg atcatttacg gcgcctttcc ggttggaagc
     1261 atcttgatga ttctcgggta cgccgaaacg gccttgcgcc attcagtgca aaaacctatt
     1321 caagggtcgg acatctcctt ggaccagggg aggcacgact cctaatgctg accacgctct
     1381 gtaccctcgg gggtttgctg cttctgctca atgtgcctat gttcgttctg atggtggcga
     1441 catccgccgt catcatctgg gcttatgacg tagcccccct catgctcatt gccacccaga
     1501 tgtttggcag catcgacaaa tttgcgttga tggccattcc cttctttatt ttcggcgcaa
     1561 gtatcatggg cgcaggagga ctgaccacga ggctcatcgc ctgggtagag gctatggttg
     1621 gatgggtgcg gggagggctt gcctatacca ccattctcac ttgcgagatt tttggtacgg
     1681 tttcgggctc gagtgcggcc acaaccgcag ccgtggggtc aattctctac ccttcgctcg
     1741 taaaacgccg atatggagag gtcttctccc tgggcctcgt cacctgtacg ggcgcgattg
     1801 ccagcgtgat ccccccgagt attggaatga ttgtttattc ggccattacc ggagcttccg
     1861 tgggggccgt ttttatgggg ggagtcggcc cgggaatact cctgggaatt tgctttgctg
     1921 ggtgggtcgc tgctcacaca cgccgggaaa aagtgcctca gatgggctca ttcgattttc
     1981 gcctggtgcg ccagagaacc caatccgccg gctgggcctt gggaatgcct ctgctcatat
     2041 tgggagggat ttattcaggc gtctttaccc cgacggaggc ctccgccatt tccacgattt
     2101 atgccatgtt cgttgcgttt ttcatttaca aggaaatgac cctgaagaag ctttttcaga
     2161 ttgccattga atccgggctg atcaccgcca agatcatgat catcattgcc tcctccggcg
     2221 tctttgtatg gatccttact gtcggcgggg ttcctgatat ggtgaataac tgggtccaga
     2281 gcgtgggcgc atcgcagttc ctggtccttt tattgatcaa cgtcgtcttc ctcatcgccg
     2341 gcatgttcct tgaacccaat tctacgcttg tggtgctgac gcctcttttt tacccgatcg
     2401 ccacgcgtct tggagtcgac cccgtccacc ttggagtcat attggttttg aatatctcca
     2461 tcggcatgta cactcctcca ttcgggttca atctttttgt cagcatgagc atattcaatg
     2521 cgccgatgcg aaagatcata gcagggctga cgcccttcat catcgtgagt cttcttgctc
     2581 tgttcatcac aacttatgtg ccccagatca ccctctggtt gccgaagatt ctctatccca
     2641 agagttttcc ctgagaacct cttgaaattg cgacctccgg cagagccgga ggtatgagaa
     2701 acaggggtta ccgggtgaaa atttaagcta gaatttgttt ttgtgcagcc agctcccttc
     2761 aggccttcgc ttggggaccg gacctccctc acccggaacc ccgaatttca ggacgcaggt
     2821 gagttcgtaa tccgccggaa gaccgaggat tttctccacc tctttttttt cttctcccca
     2881 gtaggagatg atgttgctgc caagcccctc cgccacggcc gccagtgaca tgttttctac
     2941 ggccagccat ccattggtgg cctgcccgga gctcgtgcaa acggcgacga cgctggaatt
     3001 ttggaaccat ttcttctggt tcaaagccct ttcttccact tccttttgcc cttctcccgc
     3061 tcccggagcc atctttctgt ttaattgata ctttatctcc gcaagctgat caatgatctt
     3121 ccgatcgtcg atcatgatga actcccaggg ttgacggttt ccgccagagg gtgccttcga
     3181 gcctgccaga atgattttcc gcaattgctc ttccgtggcc cctttcttga aaatacgaat
     3241 cgtcctcctc ttctcgatcg cttcgtaaag gtccattgat agcctcccga tctttgatat
     3301 tccggggcag gatcccttcc cagatgcatc cctagaaccc ccgggttttc ttatcctata
     3361 tgcataaggg ggaaaagcgg ccactgtcaa gcccaaaaaa atatagggtc ggggggatag
     3421 gtgctgggtt cgggcctgag ctggcattcc cttgccctac ttcccttttc ggcaagtagg
     3481 catgagccct attgaatttc aagcattgcc cggcccggga gtgagggcgt tgcgcgaaaa
     3541 aaccataagg cccagatcac ttcttggccc aggcgggcaa gttgttcttt ccccaagaac
     3601 atggttattc cgttataagg gcgcccggcc gggcgccaga taggcccgcc tcatcctgcg
     3661 gaaacacgat cggccctccg gttggaattc ttttcccgcc gatgcttttt ccgcgcaacg
     3721 ccctcacttc cgggccacaa gcttgctttt tcgcagaaat gaaaaacaca aaagtgaagg
     3781 gggcaggctg gcattccgga ttgacaggca gaccaaggct ctcttatact gattgctcaa
     3841 aaaaggaatt cgggccccgc cggatgagcc tgaaccatct ccaagagccg tttcagagga
     3901 ggagaggcca tgagtgagag cagtgagatt cgggatggga tgaggattga ttgggatgtt
     3961 ccgatcgcca tggacgatgg gttggaactt cgggctgata tttaccgtcc gccccaggaa
     4021 ggcaggtacc cagtaatcct aagctatggc ccctacgcca aaggcctcgc cttccaggaa
     4081 ggctatccgg atcagtggca aaggatggcc gaccagcacc cggatgtgac cgagggttcg
     4141 accaacaagt accagaattg ggaggtcgcc gacccggaga aatgggtacc ggacggctat
     4201 gcctgcgtgc gggtcgattc ccgcgggtgt ggccgttctc caggatatgt agaccccttt
     4261 tctccaaggg agacccgcga cttttacctc tgtatcgagt gggcgggaac ccagccatgg
     4321 agcaacggca aggtaggcct gtcgggcatc tcctactacg ccatcaatca gtggcatgtg
     4381 gcctccctgc agcctcccca cctggcggcc atgattccct gggagggggc tgccgactgg
     4441 taccgggaca tgacctatca cgggggaatc cggtgcgtgt tttttgggaa ctggtttaag
     4501 aaacaggtga tgaccgtcca gtacggactc ggccggaagg gatacaccaa tcccaacacc
     4561 ggcgagctgg cctccggtcc ggagacgctg tcggaggaag aactgctgcg gaatcggacc
     4621 gacctcgcgg cggccattca ggaccatccc ctggatgacc gctaccaccg ggagcgctcc
     4681 cccatctggt ccaaaatcaa tgttcccttc ctctcttcgg ccaactgggg cggtcagggg
     4741 cttcatcccc gtggaaactt cgagggtttt acccaggcag cctcaaagca gaagtggctg
     4801 gaggcccacg gcctggaaca ttggaccgaa ttttacaccc ggtatggagt caggctccag
     4861 aagcgtttct tcgatcactt cctgaaaggg ctcgacaatg attggaaggg tcaaccccgg
     4921 gtgcagctcc aggtccgaag agtggacggc tttgggcagc gctcggagaa tgagtggcct
     4981 ctggcccgaa cccggtggac ccggttctac ctcaatccct ttgaccggag cctcagccca
     5041 aaacctgtag atgctgcggg caacctagag tatgaggcac tgggggatgg tatcactttc
     5101 ctgagtcctc caatggagga agaaaccgag atcaccggcc ccctcgcagc aaaagtgttt
     5161 atgtcttcct cttcaaagga tgcagacttc ttcctggtgt tccgggtgtt ctcgcccgat
     5221 ggaacagagg tggttttcca gggagcgctg gacccccaca cgcccatcgc tcaaggctgg
     5281 ctccgggctt cgcaccggaa actggaccct gtgctcagca aagagtaccg accctatcac
     5341 ccccacgatg aggttgaacc cttgaccccc ggcaagattt acgagctcga cgtggaaatc
     5401 tggcccacct gcattgtggt gccgaaaggg taccgcatgg ctctgtcgat caggggcaaa
     5461 gactacgagc acccgggaaa gggtgcgcgg atcaagtcct tcgtgaatga gttgaaggga
     5521 tgcggcccct tcctccataa cgatccccag gacagacccg aatccctttt cggcggcaag
     5581 aatacggttt atgggggagg gaaaaaccct tccttcttgc ttgtacccat cattcccaag
     5641 cgccaggtcg gagcctcccg gggatgatgt catttattcc tgcgccgtcc acggggatag
     5701 cgacgaatgg aacaacccga gaaccagagt cccttttttt aattgacaaa gtttttcgga
     5761 catactatgg ttggattgaa attggtccaa ccattggatc attcatccat tggagggagg
     5821 cttctcccaa acactccttc ctcatgaagc aggacctccc cgaagaaagt gcaggtaccc
     5881 tttgagccga gtcggtgctc accgatcttc acccgtcccg ccgccctccc tcagcgaaaa
     5941 cgcagagacg gagaatggga gcttattgaa acccatccga agggttcggc tctacgagaa
     6001 cgcagtcgac cagattcaga ccttgatcct gagccacgaa tataagccgg gagaccgttt
     6061 gccctccgag cgggccctgg ctgagcactt ccatatcagc cggcattccc tgagggaggc
     6121 ccttcgcata ctggacgtga tgggcctcat cgaaatcaga gtgggggacg ggatctacgc
     6181 caaggaagtg gatttcctcc cctacattga atctttaaat ctctccatca gttccaggct
     6241 tcccctggag cgggacagtt ttgtcaagct ctggagggct cggagaatcc tggaggcggg
     6301 catcgtggac ctggcggcca aagaggtcac cgagtccttc ttgaagagcc tttggtggtg
     6361 catcggggag atggagaaga atattactca tcaggaggcc ttcatctccg cggggatccg
     6421 gttccatcga ctgatcgctg aagtggccca gaatgagatt ctgattttga tatgggacat
     6481 gctggccaac ctcattcgga agagccaaag caagatctac ggcatctcct ggtctccccg
     6541 gaaggctctc tccgctcata agaaaattta tctggccttg aaagccagag acccgcagaa
     6601 agcagtggaa gccatgaagc aacacatgct ggatgaggag aaggccctgg tggccgcgct
     6661 gggaaaggga aaaggctgaa ccgtctttct tcccgatcgg ggagaagaga cggcttcgga
     6721 gtcttaacta ccacaaaagg aggaaagggt tatggcaagg gacttatcat ttatcggtcg
     6781 acctcatgag tacgacgccc ggagggtatt ttccctcacc ggtgccgatt ggcaggatcg
     6841 ggtcaatttt gaccggctac gcaaggaaag gcttgctcga gccaggcagc agatggagat
     6901 tcatgacctg ggagccctgg tggtttatga cggggcgaat gtccgttatc tgaccagttc
     6961 ttttcaggga aactggaaat acaacatctt catccgttac gccgtcctgt gccggggcgc
     7021 ggaacccatc ttattcgaga cggccggctc cgacctgcag tgcgccaaga tcgacctgcc
     7081 atggatggag ggcaggatcc gcccggctat cacctggaaa tggtccgagg gagcggtgga
     7141 gtacatggtg gaccggatgg tggacagcgt ggtcgaggtc ctgaaagaaa ataaagtgga
     7201 gaaagagaag atcgggatcg attcgctgga catggcttct ctggccgctt tccagaagaa
     7261 gaagatcaac gtggtcaacg cctggccggc catgtccgcc gcccgcgtcg tcaagaccca
     7321 ggatgagatc gaactcctga aacagtccgc ggccatcggc gacgcctgca tgtacaaagc
     7381 ccggtatgaa tgggccaagc cgggtatcac cgaacgggag ctctccggaa agatgatcga
     7441 gtacatgtac tccaagggtt gcgagatcgt ctacgagatc atcgtcgcct ccggcgggaa
     7501 tacgagcccc taccggcgct ggcccacgga caagatgatc cgccagggag acttgatcat
     7561 catcgacaac aacaccgtcg ggccttccgg atacttcgtg gactacgtgc gctgctggaa
     7621 gattgaagct aagcctaccc ccaaggagaa ggacctttac aaggagtgtt acgattccct
     7681 gatgggggcc attgaaatga tgaaacccgg caaaacgagc aaggacgtgg ccgaaaagtt
     7741 cccggtctat gatgacgaca agtacgggac cgtgaccctc cagcagttcg cccattccat
     7801 cggcctgacg ctgtacgagg ggatgtggat ttcccgggcc tattccatga aatacccggc
     7861 ggagattaag cccaacatgt acttcgcggt ggaaactttt gccggacacc cgttgctgga
     7921 acaaacggtt cgactggaat gtgatttggt gatcaccgac aagggacacg aactcttcac
     7981 cctctttccc ttcgaagagg atatgcttaa gtgaaaaatc ttggaaaaac aagctcaagg
     8041 ccaaatccca tcccggccgg ccggatcacc actctggcga agagagcggg ccggctggcc
     8101 gaaaagcagc gaaagacggg ctaccattga agcgaatcgg tcttcgctgc ggtcgcggcg
     8161 gtcctggaac ttagagtcaa gccggcccct cccaagatag cctcgggttt tcatggaggc
     8221 ggcggaggcg taaagaccga cgaggaaggg atgaaaggaa ctcccaccgg ttgcatgtgt
     8281 ggggccctgg cggccggcgt catcgtgctg ggtgtcctgt atgggcgctc cgcgccagtg
     8341 gggcccaggt ttggatgcat cagtcatctt tccgctcatc tccacaaacg gtttcaagac
     8401 gaattggggg gaaagtgttg cgctatgctg cgcccgttct atcagaagat ggaccgggaa
     8461 cattcctgca gtcacatcta ccagaaaggg gctgagctgg ccgtggaagt ggccctttca
     8521 gctccctctc tggttccgga ctgccggatg ccgaggcctc tccatgcgtt gctgaagaag
     8581 aagcggccgg gcaatgggca atgatcaatg gtcaatgcgc aatttacgat gattaggtaa
     8641 gacgcgtaga gattcctcat aagaacagaa aaagggggta aaaggtatgg gcggaagatc
     8701 gattcgtacg gtcatcatcg gaatggtggc agggctgatt cttttcaccc cgctcgtttc
     8761 ctgggggcag gccaagggac cgatcaagat cgggttcatc tccccccttt ccggggggat
     8821 ggcggccaac ggaaaggaca tgctggccgg gattcagctt tatcttgagg agatcggcta
     8881 ccaggcggcg ggccggaaga ttgagctcat cgtcgaggac gacgaggcga acccggcgac
     8941 cgggctgacc aagacccgga aactggtgga gaaagatgag gtccacgtca tgagcggggg
     9001 cctgatggcc tccacgggat acgccctggt gccgtacatc gactccaaag aaatccccat
     9061 gacctaccct atcatggctc cggatgacct gacccagcgg cagagggcca aatggatcgt
     9121 caggacggga tggaacagca gccagcccaa ccatcctctg ggagaatatg cctaccaggt
     9181 tctcaaattc cggaaagtga tcatcatcgg gctcgactac gccttcggat gggaatgcgc
     9241 gggaggattc cagaagacct ttgaggatgc gggcgggaaa gtgatccaga agatctggac
     9301 cccgctgacg gtaaccgatt tcagccccta cctcgcccaa ttctccaagg aagcggatgg
     9361 ggtttatgcg atgttcacgg gccgcacgac gctccaattc accaagcaat accaggaatt
     9421 cggcctgaag ggaaaactcc ctctgatcgg tggagggaca accacggatg aacacgccct
     9481 tccttccatg ggggatgagg cgattggaat cataacctct ctgcattaca gcgaggctct
     9541 cgacaacccg gccaacaaga aattcgtgaa ggcctaccgg gaaaaggcca agaaggccgc
     9601 ctcctactac tcggagggga cctacacggg ggcgcggtgg atcgtagagg ccatcaagaa
     9661 cataaacgga gacgtggaaa acaggcagaa attcatggac gctctgagaa aagtggaact
     9721 ccgggatgtt ccccgcgggt ccttccggct ggatgactac ggaaacccgg tgcagaatgt
     9781 gtatatccga aaggtggaga gggtcggggg agagctccag aatacggtga ttcacacgat
     9841 tcccaacgtc tcccagttct ggaagtacaa acccgaggag tttctgaaac agcccgtcta
     9901 ttcgagggag tttccgccgt tgaagccgtg agcagggggt ttgagttaac acctatatca
     9961 ggagaggagg tttggagcca tgaagaaagc attgttcagt attctctgga tggtggcggc
    10021 ggtgagttta gtccttttga atccttcggt ttcaccagcg caggggaaag gcccgatcaa
    10081 aatcgggtac atctctcctc tttcgggagg gatggcggcc aacggaaaag acatgctagc
    10141 agggattcag ctctatcttg aagagatcgg gtcccaggcc gccggccgaa agattgagct
    10201 catcgtggag gacgacgagg cgaatccggc taccgggctc accaagaccc ggaagctggt
    10261 ggagaaagat ggggttcacc tcatgaccgg gggtcttatg gcttccacgg cctatgccct
    10321 tgcgccctac atcgactcc
//
LOCUS       DASPRR010000027        11145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_9461, whole genome shotgun sequence.
ACCESSION   DASPRR010000027 DASPRR010000000
VERSION     DASPRR010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11145)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11145
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9461"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VEL68_00950"
     CDS             complement(<1..1355)
                     /locus_tag="VEL68_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXZ34564.1"
                     /translation="MSSMKGARRAFLEEKFGNRVTFQRLERKLYGHDIAAMPALFKPL
                     IGNTTPDGVVQPESESELVELVEFARQNGIPLIPRGKATSGYGGAVPLKQGLVVDFYR
                     MKKIIGIDPQNQTATVEAGVVWEKLDQELAKHNLTLCLYPTSYPSSTVGGWLAQGGAG
                     IGSYECGWFRDNVLSARVVMPNGQVKEFNGQDLELISEAEGITGFISRITVKVQPLED
                     LEVISIGCPHPQDLQRLIESMIAARLPIWSLMFINPRMAELKNRAPLMEHNGHTIEER
                     LLLPAAYIMTLTFRKKDREAIGGKLAEIIKLCEAEILSERIADHEWKNRFKLMVVKRL
                     GPSLVPAEVVVPLDHLGTVMTQIERQIDQPLVKEGLVIPKGNGGTPEVVILGFIPSDQ
                     RKLSYNFVFGLVLTLMKIAEKNGGRAYSTGMYFTRKAAQVLGADRLQRMKGFKNQVDP
                     KG"
     gene            complement(1553..1840)
                     /locus_tag="VEL68_00955"
     CDS             complement(1553..1840)
                     /locus_tag="VEL68_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HXZ34565.1"
                     /translation="MKNNGEERSNILLRLRRIEGQVRGLQRMVEEEAPCPEVLTQVGA
                     VTAALKKVGMVMIATHMEKCMAEMAQKTDGNKQRETLRDFQKAMSRYIGWA"
     gene            complement(2043..3512)
                     /locus_tag="VEL68_00960"
     CDS             complement(2043..3512)
                     /locus_tag="VEL68_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ34566.1"
                     /translation="MALMTPDQYVASLKRMKPRVFMNGKKVDSILDNKNTRTVVESNK
                     ASYTWALDPRYKDIMTSYSPLIDEVVNRYTYLSASVEDLVKKAQAGTFTSEMLGTCTY
                     RCVGYDAFHSLASTTWEMDRDLGTKYYPRFIEFLKTVQKKDLSVAGALTEPRGKRNQK
                     TSEWADPHLSLKIVDRKKDGIVVRGAKINISGAYAAHELVVLPQSAHSQDEVDYAVAF
                     AIPPDTKGMTFVAQYTPFSAEREMCDDIEELGNPLFGQRETSMVIFEDVFIPWERVFH
                     CGETPYSGKLVTRFARTHRMTCGGTCKVGFMNQIIGAAKLIQEYKGLEKVAHINEQLA
                     EMVVLRETGRACGLAAAMKGAEEPPGSGVFLPDEMMGNVAKLNICNAFWRVMALAGDI
                     GGGLVVTMPSMKELKNPEVQKYVEEFYSFGSDEPTENIMKVHKALQHWTAGLHGVGTW
                     HGAGPVMAQKIMLQRIANYEHEKELVKRTLNLKEKKGKG"
     gene            complement(3653..4417)
                     /locus_tag="VEL68_00965"
     CDS             complement(3653..4417)
                     /locus_tag="VEL68_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ34567.1"
                     /translation="MRFQLLRHATAVIAVNRLNLLLDPMLSQKESMGPIVNAANQNRI
                     PMVDLPLTEPELRGLIDRLNGVLVTHVHRDHWDGRAAELLPKQLPVICQEEDEPVMRK
                     AGFAQVYPVKTKLEWQGIQIFRTGGQHGTGEIGKKMGIVSGYVLKAEGEPALYIAGDT
                     IWCPEVKEALQTHKPEVVILYSGAAAFLAGGPITMNADHVIAVCRELPSAKVIVIHME
                     TMTHCLLTRTELRKRLEQEGLSGQVLIPRDGEAVSL"
     gene            4939..6096
                     /locus_tag="VEL68_00970"
     CDS             4939..6096
                     /locus_tag="VEL68_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ34568.1"
                     /translation="MNFKRLLPIYLGAIIGPMGGVGIITLLPVLCRHFDISIQWASLT
                     VTLYMIPYVVFQLFSGSIAQVFDTRRTLLFGFGTYALGSFLCGLSSDLTTLITVRFIQ
                     GFGAAFIAPIVLALVGEMVDSKHTGRAMGVLGVMYTIGVTMGPLISGVLEVSLGWPWF
                     FFFLMLLSLSIGGFYWVTSQAEERPNAKAGKISDALALVKKSYSYQRVKYLSLAAFSL
                     FLGYIGLMTFVADYLKVTFDLASDKTGLILSMTGFFGIMAAPVAGILGDRRGRLPVAC
                     LGGGIMVAAILGLAAVEYSYEKYLLLFAVFGAGSATAWTSLNTLAVEVVPDLRKPVAS
                     VYNCFKFAGYALSPLVLSLLYIPFSISGVRWACIACILVSLLLASRIRTAK"
     gene            6427..7332
                     /gene="dapA"
                     /locus_tag="VEL68_00975"
     CDS             6427..7332
                     /gene="dapA"
                     /locus_tag="VEL68_00975"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXZ34569.1"
                     /translation="MQPKTWAGCHIPLITPFKADYSLDVDGLKKLVDYFIEEEKCDGL
                     VPCGTTGESPTLTNEEHDKVIELTVKFARGRVPVMAGTGSNSTAEAIERTKHAEHVGA
                     AASLQVCPYYNRPTQDGIVAHFQAVAKATKLPLFIYNIPSRTGRLIEAKTMIELSKID
                     NIIGMKDACGDMMVTMDIMRGTRGGGKKFYVLSGEDALTFPMMTLGGDGGIMAVANVI
                     GKEYREMIHLMLAGKLEQAREIHYKTLQVVRTLFIESNPAPAKEAMNMMGLPAGPLRL
                     PLVPLRPENRETLRKALVQVGRLKG"
     gene            7337..7672
                     /locus_tag="VEL68_00980"
     CDS             7337..7672
                     /locus_tag="VEL68_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXZ34570.1"
                     /translation="MEIQVRSKVWLEAQGRPVLGPGRLELLKYIDEQGSISRAARLLK
                     MTYRKAWGQIKAMEEQMGLPLVQKQTGGRGGGGTRLTPEARRLLSKYILLAQGIEEEV
                     DRRFQEIFL"
     gene            7783..8799
                     /locus_tag="VEL68_00985"
     CDS             7783..8799
                     /locus_tag="VEL68_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013553111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ34571.1"
                     /translation="MSALKFSYLAVRPEPVEGRTGDYGTVSRVGGRGWGGLIGRPSKG
                     STPLHDNLSHALLSIVVVMLATAIIVAPSHASSAPLLIFAGAASKPPTEEVARAFGQR
                     AGIQVRVTFGGSGFVLSQMRLARMGDVYFPGSSDFMEKAKREKLVFPETEKIIAYLIP
                     AINVQRGNPRNIRSLRDLTQPNLRLAIADPEGVCVGTYAVEVVEKSLRPEERERFRKN
                     LATTVESCEKTANIISLKMVDAVIGWRVFQHWDPERIETILLKPEEVPRIGYIPAAVS
                     TFTRERLLADEFIRCLTQTEAREIFRKHGYLMSVEEARAYTLPGTPVGGEYVLPGSWK
                     RRGK"
     gene            8842..9618
                     /locus_tag="VEL68_00990"
     CDS             8842..9618
                     /locus_tag="VEL68_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXZ34572.1"
                     /translation="MNLGKTAIAAALALFLFYAILIFSLLTFLEGERFLEVLLAPDTL
                     FSIRLSIITATLAMVLAVAIGLPSAYALSRFQFPGKKLVDTLLELPLIMSPVALGAAL
                     LVFFNTPFGNLIQEKGILFIFEFPGIVLAQFATIAGLATRLMKTALDEISPRYEAVAR
                     SLGASPWEAFRKITLPLSLRGILSAMILCWAKAVGEFGATVTLAGTMAFKTETMPIAI
                     FMSLASADIPKTIILILILAGIGLTALYAVRLVEKRPQVL"
     gene            9615..10715
                     /locus_tag="VEL68_00995"
     CDS             9615..10715
                     /locus_tag="VEL68_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34573.1"
                     /translation="MIEVRGLDLSLGDFRIENLNLTVKEEAFNVLLGPTGAGKTLILE
                     CIAGLRRIHKGQVLVGEEEVQDLPPEKRGVSYVPQDLALFPHLTVERNLVFGLRTSPK
                     SLFKDDPHLARLVETLKIEHLLQRYPQGLSGGEKQRVALGRALAPYPRLLLLDEPLAA
                     LDPALKGEIQQLLLSLHRSIGFTALHVTHDLDEAYLLGERINVLIHGNLEQSGTKEDV
                     FLHPQTAGVARFLGLRNLFRARVLRRDDGRKYITAGVWGKEVMIPLEHCARSPLPGEE
                     VFLYLRPEEVMILRPGKQVKESLRQNIFAGQVERVLDRGTHRWVLFRPLGLEVHLEIN
                     LPNYVFRNLGLGEGKEIQVAMRRESFWVIPTK"
     gene            complement(10785..11033)
                     /locus_tag="VEL68_01000"
     CDS             complement(10785..11033)
                     /locus_tag="VEL68_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HXZ34574.1"
                     /translation="MIVSSGFLLIDVSFKDSILKELEARGIELHGVKEEKVVFLAEGE
                     NAGELKARMESLQGIEGVQGVYLTYFSLDEEKSEKNEE"
BASE COUNT         2309 a         3203 c         3064 g         2569 t
ORIGIN      
        1 cccttggggt ccacctgatt tttgaagcct ttcatccgct gaagacgatc ggcccccaat
       61 acctgggcag ccttccgggt gaaatacatg cccgtactgt aagcacggcc cccattcttc
      121 tcggcgattt tcatcagcgt caggacaagt ccgaacacaa aattgtaact gagcttccgc
      181 tgatcgctgg gaatgaatcc caggatgacg acctcggggg ttcctccatt ccccttcggg
      241 atgaccaggc cctcttttac cagcggctga tcgatctgtc gctcaatctg ggtcatgacc
      301 gttcccaaat ggtcgagggg aaccaccact tcggcgggca ccagggaagg cccgagtctc
      361 ttgacgacca tcagcttgaa acggttcttc cattcgtgat cggcgatgcg ctcgctcaga
      421 atctccgcct cgcataattt tatgatctcc gccagcttcc cccctattgc ctcccgatcc
      481 tttttgcgga aggttagagt cataatgtaa gcggcgggaa gcagcaggcg ttcttcgatc
      541 gtgtgtccgt tatgctccat gaggggagcc cgattcttca actccgccat gcgcgggttg
      601 ataaacatga gagaccagat cggcaaccgg gccgcgatca tggattcaat caagcgctgg
      661 aggtcctgcg ggtgagggca gccgatggaa atcacctcga ggtcttccag gggttgaact
      721 ttcacggtaa tccgggaaat aaaaccggta atgccttcgg cttcgctgat cagttccaag
      781 tcctgaccgt tgaactcctt gacctggcca ttgggcatca ccacccgggc gctgaggacg
      841 ttgtcccgga accatccgca ttcgtaggag ccgatccccg cccctccctg ggcaagccag
      901 cccccgaccg tagaagaggg gtaactggtc ggatagaggc acagggtcag gttgtgtttg
      961 gccaactcct gatcgagctt ttcccatacc acccccgcct cgaccgtggc cgtctgattc
     1021 tgcgggtcaa ttccgatgat cttcttcatc cggtagaagt ccacgaccag tccctgtttc
     1081 aagggaacag ccccgccata tcccgatgtg gctttccccc gaggaataag aggaattccg
     1141 ttttgccttg cgaattcaac caactcgacg agctcggatt cggactccgg ctggacgact
     1201 ccgtccgggg tggtatttcc gatcaggggc ttgaacaggg caggcattgc cgcaatgtcg
     1261 tgcccataga gtttgcgctc caatctttga aaggttaccc ggttaccgaa tttttcttcc
     1321 agaaacgccc gccgcgcccc cttcattgaa ctcatgactg aacctcctgc acgaataaaa
     1381 ttgaaatcgt aatcaaggac cgatgtccgg catttcaccc aacctgcgtc tcttattggg
     1441 gatgagggcc taggaccctc aaaaaatatt ggggtatccc cccctcacct gaggcggcaa
     1501 ggggatagga ccgagaaaag gcggtaggga agaatccacc ctacccaaaa gatcacgccc
     1561 agccgatata tcgagacatc gccttttgaa agtcccttag cgtctcccgc tgcttgttcc
     1621 cgtcggtctt ctgcgccatc tccgccatac atttctccat atgggttgcg atcattacca
     1681 ttcccacttt ttttaaagca gcagtcacag cgccgacctg ggtcaggacc tccgggcaag
     1741 gcgcctcttc ttccaccatc ctctgaaggc ctcggacctg accctcaatc ctccgaagcc
     1801 tcagcaggat gttcgatctt tcctcaccat tattcttcat actatacccc cctatggtat
     1861 taaggccatc ataaatcgag tccttccgtg ttgtcaatcg aaatttcgct cggcctggaa
     1921 ttccgccaaa ggaaaatgga tggggttaaa aagaagccat aaggccggaa tggagtggga
     1981 gaatgaccgg gggaggcggg gggcaggtca tattttgaaa gatatttttg gggccaggga
     2041 gcctatccct tccccttttt ctccttcagg ttgagggtac gtttgaccag ttccttttcg
     2101 tgctcgtagt tggcgatccg ctggagcatg atcttctggg ccatgaccgg gccggcccca
     2161 tgccaggtgc ccacgccgtg gagtccggcg gtccagtgct gcagggcctt gtggaccttc
     2221 atgatgtttt ccgtcggctc gtcggagccg aagctgtaga attcctccac gtatttttgc
     2281 acttccgggt tcttcagctc tttcatcgaa ggcatggtga ctaccaggcc gccgccgata
     2341 tcccccgcca gggccatgac ccgccagaag gcgttgcaga tattgagctt ggccacattg
     2401 cccatcattt catcgggcaa aaagaccccc gatcccggag gttcttcagc gcccttcatg
     2461 gccgcggcca gaccacaggc gcgcccggtt tcccgcagga ctaccatctc ggccaactgc
     2521 tcgttgatgt gagcgacctt ctccagccct ttgtactcct ggatgagttt ggccgctccg
     2581 atgatctggt tcatgaagcc caccttgcag gttccgccgc aggtcattcg gtgggtgcgg
     2641 gcgaagcggg tgaccagttt gcccgaatag ggggtttccc cgcagtggaa caccctttcc
     2701 cagggaatga acacatcttc gaagatcacc atcgaagttt cccgctggcc gaagaggggg
     2761 ttgcccagtt cctcaatgtc gtcgcacatt tccctttccg ccgagaaggg ggtgtactgg
     2821 gcgacgaagg tcatgccctt ggtatccggc ggaatggcaa aagccacggc gtaatcgact
     2881 tcatcttggg aatgagcgga ctggggcaaa accactagct catgtgcggc atacgccccg
     2941 ctgatgttga ttttagcccc ccggaccacg attccatcct ttttccggtc cacgattttg
     3001 agagagagat gagggtccgc ccactccgag gtcttctgat tccgttttcc ccgcggttcg
     3061 gtgagggctc cggcaaccga gaggtccttc ttctggacgg ttttcaggaa ttcgataaag
     3121 cggggatagt atttcgtacc caggtcccgg tccatttccc atgtcgtgct ggccagggaa
     3181 tgaaaggcgt catagccgac gcatcggtag gtacaggttc ccagcatctc cgaggtgaac
     3241 gttccggcct gggctttttt taccaggtct tcgacgctgg cgctcaaata ggtgtatcgg
     3301 ttcactacct catcgataag cggcgaatag ctggtcatga tgtctttata cctggggtct
     3361 aaggcccagg tgtaactggc tttgttggat tcgaccacgg tccgggtgtt cttgttgtcg
     3421 aggatgctgt ccaccttctt tccattcatg aagacccggg gcttcatcct tttcaggctt
     3481 gctacatatt gatcaggagt cataagagcc atcgcatcct cccaaattcg aaattcgaat
     3541 atcgaaatcc gaaaccaaat tccaaatcca attccaaact ctttgttccg atcattcgaa
     3601 tttctaattt atgcataaca tgcaaggatt aggctgtcaa attcctggga cttcagagag
     3661 agacagcttc tccatcccgg gggatcagca cttgtccgga cagcccctcc tgctccagtc
     3721 tctttctcag ttcggtgcgg gtcaggaggc agtgggtcat ggtttccatg tggatcacga
     3781 tcaccttcgc ggaaggaagt tcccggcaca ctgctatgac gtggtcggca ttcatggtga
     3841 tcggcccgcc ggctaaaaat gcggccgccc cggaataaag aattacgacc tccggcttgt
     3901 gggtctgaag cgcctctttc acttccgggc accagatggt gtccccggca atataaaggg
     3961 cgggttctcc ttcggccttc aggacataac cggaaacgat tcccattttt ttcccgatct
     4021 ctcccgtccc gtgttgccca ccggttcgaa aaatctgaat cccctgccat tcgagcttgg
     4081 tcttaaccgg atagacctga gcgaaccctg ccttcctcat gaccggctca tcctcctcct
     4141 ggcagatcac gggcagttgt ttgggaagaa gttctgcggc ccggccgtcc cagtgatcgc
     4201 gatggacatg ggttaccaga accccattca accggtcgat caatcctcgc agctcaggct
     4261 cggtcaatgg caggtccacc atggggatcc ggttttggtt ggccgcattg acaatcggac
     4321 ccatggattc cttctgactc agcatgggat cgaggagaag attcaggcga ttcacggcaa
     4381 tcactgcggt ggcatgccgg agaagttgga agcgcatggt cgttctctcc ctgcaaaagg
     4441 attcggattc cttagatgag gaatgatttc atcttacctt tttttcattg ccttttcacc
     4501 cgggatttgt tcaaaaattt tccgcactcc gccacttgcc cacccccagt tcttaaaaaa
     4561 gagtccttca aaaataagat ctctggaaag gaggggggat ctcatggggt tcagggcgga
     4621 gaaatttgga tcatcatcct gaggcggtgg ctcttccatt ggatcgggcc tgtcctgggg
     4681 ggattgaaac ggaaacatgc tttggctctc ttttctgctt ggaggaatct cctctcgggc
     4741 ctctgtctgc ggaacatggg gaggagtttc ctggaagggt aagcacccgc cgagcctgat
     4801 gatccaaatt cctccaccct gaaccccaac aagcacgaaa aactgggggt gggcaagcac
     4861 tccgccaaga ttgacatctg ttttttattg ttataacgtg cctttcatta actctttctt
     4921 tcctggactg gtttgctcat gaattttaag cgcctcctgc ccatttacct gggggccatc
     4981 atcggaccca tgggaggagt cgggatcatt acgctcctcc ctgttttatg tcggcatttt
     5041 gatatcagta tccagtgggc ctcgctgacc gtcacccttt acatgatccc ttatgtggtc
     5101 tttcagctat tttccggctc catcgcccag gtctttgata cgcgtcggac tcttcttttt
     5161 gggttcggta cctacgctct gggcagcttc ctgtgcgggc tctcttccga tttgacaacg
     5221 ctcataaccg tccggttcat ccaagggttt ggagcagctt tcatcgcccc catcgttctg
     5281 gctctggtcg gggagatggt ggattccaaa cataccggga gggccatggg ggtcctgggg
     5341 gtcatgtaca ccatcggagt gaccatggga cccctgattt ccggagttct ggaggtttcc
     5401 ctagggtggc cctggttctt tttctttctc atgctcctct ccctctccat cggggggttc
     5461 tactgggtca caagccaagc agaagaaaga ccgaacgcca aggcgggaaa aatctccgat
     5521 gctctggcgc tggtcaaaaa gtcctactcc tatcagcggg tgaaatattt aagcttggct
     5581 gccttctctc tattcctggg atacatcgga cttatgacct ttgtggccga ttatttgaag
     5641 gtaacctttg acctggcatc cgacaagacg ggcctcatct tatcgatgac cgggttcttc
     5701 ggcattatgg cagcgcccgt ggccggtatt ctcggggacc gtcggggaag acttcccgtc
     5761 gcttgcctgg gaggagggat tatggtggcg gccattttag ggctggcggc ggtggaatac
     5821 agctatgaga aatacctgct gctctttgcc gtcttcggcg ccgggtctgc cacggcctgg
     5881 acttctctga acacgctggc cgttgaagtt gtcccggatt tgcggaaacc cgttgcttcg
     5941 gtctacaact gctttaagtt tgccgggtat gctctctctc cgctggttct ttccctcttg
     6001 tatattccct tctccatttc cggggtgcgc tgggcctgca tcgcctgcat cctcgtcagc
     6061 ctccttctcg cctcccgcat ccggaccgca aaatagaaag ggggagggta gaaaataatg
     6121 aagggcgacg caattttttg ttgacaggaa ttcccactcg ggtaaaatag tttttaattt
     6181 ttaaggggaa aaaatataga gcgatgatcg gtcaccggca ggaaggaaga aaaaagctag
     6241 gcaagaagcc tgagtgcaag aaattgggcc tacccatcgc ttttgggatg ccttaaccgg
     6301 cccctgctcc ccgccaccat cctaaaagcc atgggcaaag gccccatggc ttttttgttt
     6361 tgaaagcctg ggccgcactg tgcgccctgg ggtggaaaat tcataaataa aattaaagga
     6421 ggagttatgc aaccgaaaac ctgggccgga tgtcacattc cattgatcac ccctttcaaa
     6481 gccgactatt ccctcgacgt ggatggactc aagaagctgg tcgactattt catcgaagag
     6541 gaaaagtgcg acgggctggt tccctgcggg acaacagggg aatcccccac cctgaccaat
     6601 gaggaacacg ataaagtcat tgaactcacg gtgaaattcg cccgcggccg ggtgccggtc
     6661 atggccggaa cggggtccaa cagcacggcc gaggccatcg agaggactaa gcacgccgag
     6721 catgtggggg cggccgcctc cctgcaggtc tgcccctact acaaccgtcc cacccaggac
     6781 ggcatcgtgg cccactttca ggccgtggcc aaagccacca agctgcccct cttcatctat
     6841 aacatccctt cccggaccgg aaggctcatc gaagccaaga ccatgatcga gctttccaag
     6901 atcgacaaca tcatcgggat gaaggacgcc tgcggcgaca tgatggtgac catggacatc
     6961 atgcggggga cccggggcgg ggggaagaag ttttatgtcc tctccggcga agacgcccta
     7021 accttcccca tgatgaccct ggggggggac ggagggatca tggccgtggc caacgtcatc
     7081 ggtaaggagt accgggagat gatccatctg atgctggcgg ggaaactgga acaggcccgg
     7141 gaaatccact ataaaaccct gcaagtggtg aggacgctct tcatcgagtc gaaccccgcc
     7201 ccggccaagg aagccatgaa catgatgggc ctgcccgcgg gcccgcttcg cctgccgttg
     7261 gtcccgctcc ggccggagaa ccgggagacc ctgcgcaagg cgctggttca ggtgggccgt
     7321 ctgaagggat aaaggattgg aaatccaagt tcgttccaaa gtctggctgg aggcccaagg
     7381 gcgaccggtt ctgggacccg gccgcctgga gctgctcaag tatatcgatg agcagggttc
     7441 catttcccgg gccgcccgcc tgctgaagat gacctatcgc aaagcctggg ggcagatcaa
     7501 agccatggaa gaacagatgg gtctgcccct ggtgcagaaa caaaccgggg gacggggggg
     7561 agggggaaca cgcctgaccc cggaggctcg ccggctcctc tccaaataca tcctcctggc
     7621 ccaggggatc gaggaggagg tcgaccggcg attccaggag atttttttat gaactgcgtt
     7681 aggcagattt tgcatagccc ttttcacccc cacccaaccc tcccccctcg gagactgtgt
     7741 cgtaatccgt tcatggttcg acaagctcac cacgaacgga ttatgagcgc attgaaattc
     7801 agctatttag ccgttcgccc tgagcctgtc gaagggcgaa cgggggatta cggcacagtc
     7861 tctcgagtgg gagggagagg gtggggggga ttgattggcc gcccctcaaa ggggagcact
     7921 cctcttcatg acaacttatc tcatgcattg ttatcgattg tagtcgtcat gctggccaca
     7981 gcaataattg ttgccccttc ccatgcttcc tccgcgcccc ttctgatttt cgcgggagcg
     8041 gccagtaaac cgcccacgga ggaggtggcc cgggctttcg gacagcgagc gggcatccag
     8101 gtccgggtta ccttcggagg ttcgggcttt gtcctctcgc aaatgaggct agcccggatg
     8161 ggggatgtct attttccggg ttcttccgat tttatggaga aggccaagcg ggaaaaactg
     8221 gtctttccgg aaacggaaaa aatcatcgcc tatcttattc ccgcgatcaa tgtccagaga
     8281 gggaatccgc gaaacatccg ttcccttcgg gacctgaccc agccgaacct gcgcttggcc
     8341 attgccgacc cggagggggt ttgcgtgggt acctacgccg tcgaagtagt cgagaagagc
     8401 cttcggcccg aggagaggga gcgatttcgc aagaatctgg ccacgacggt ggagagctgc
     8461 gagaagacag ccaacatcat ctccttgaaa atggttgatg cggtcatcgg ctggcgggtt
     8521 ttccagcact gggacccgga aaggatagag accatcctcc ttaagccgga ggaggttccc
     8581 cgaatcggct acatccccgc ggcggtctcc actttcaccc gggaacggct gctcgcagat
     8641 gaattcatcc ggtgcctcac ccaaaccgaa gccagggaga ttttcagaaa gcatggatac
     8701 ctgatgagcg tggaggaagc acgggcctac acccttccgg gaactccggt ggggggagag
     8761 tatgttctgc cgggaagctg gaagaggagg gggaagtaag gacgcggaga cacgcgggcg
     8821 cggggacacg gagaaaaaag tatgaatctt gggaagacgg cgatcgctgc ggcattggcg
     8881 ctctttttat tctatgctat tctgatcttc tctctgctta cctttctgga gggggaaaga
     8941 tttctagagg tgctcctcgc tccggatact ctcttctcga ttcgcctgag catcattacc
     9001 gccaccctgg ccatggtcct ggccgtagca atcgggttac cctccgccta tgccctttcc
     9061 cggttccaat tccccgggaa gaagttggtg gacaccctgc tggagcttcc cctgatcatg
     9121 tccccggtcg ccttgggggc cgccttgctg gtcttcttca acaccccttt cggaaacctg
     9181 attcaggaga aagggatcct gttcatcttt gaatttccag ggatcgtcct ggcccagttt
     9241 gccaccatcg ccggattggc gacccggctc atgaagacgg ccctggacga aatctctccc
     9301 cgttatgagg cggtggcccg ttccctgggg gcgagcccct gggaagcttt tcggaagatc
     9361 accctgcccc tgtccctgcg gggaattctg agcgccatga tcctctgctg ggcgaaagcg
     9421 gtgggagagt tcggggccac ggtcaccctg gccgggacca tggcctttaa gaccgagacc
     9481 atgcccatag ccatcttcat gagcctggcc tcggcggaca ttcccaaaac catcatcctg
     9541 atcttgattc tcgccgggat cggactcaca gcgctgtatg cagttcgcct ggtagaaaag
     9601 aggccccaag tcctatgatc gaagtgcggg gactggacct ctccctggga gatttccgga
     9661 tcgaaaatct aaacctcacc gtaaaggagg aggctttcaa cgtcctcctg ggcccgacgg
     9721 gtgcgggaaa gaccctcatc ctggaatgca tcgcaggttt aaggagaatc cataaagggc
     9781 aggtccttgt cggcgaagaa gaagtccaag acctgccccc ggaaaagcgg ggggtctcct
     9841 acgttcccca agatttggcc ctattccccc acctcaccgt ggagaggaat ctcgttttcg
     9901 gactgaggac ctcccccaaa agccttttca aggacgaccc ccaccttgcc cggctggtcg
     9961 agaccttgaa gattgaacac cttctccagc gctaccccca agggctcagc ggcggggaga
    10021 aacaaagggt cgccctggga agggctctgg ccccttaccc ccgactgctc cttctggatg
    10081 aacccctggc cgccctcgac cccgctttga aaggagaaat ccagcaactg cttctgtcct
    10141 tgcaccggtc cattggcttc acggctctcc atgtgactca tgacctggat gaagcctacc
    10201 tcctgggtga gagaatcaac gtgctcatcc acggcaacct ggaacagagc ggtacgaagg
    10261 aagacgtctt cctccatcct caaaccgcgg gggtggcccg ctttctcggt ttgcgaaatc
    10321 tcttccgggc gcgagtattg aggcgggatg acgggcggaa atacataacc gcgggggtct
    10381 ggggaaaaga ggtaatgatc cccctggaac actgcgccag aagtccttta cccggcgaag
    10441 aggtttttct ctaccttcgc ccggaggaag tgatgattct gcggccgggt aaacaggtca
    10501 aggaatccct tcggcagaac atctttgccg gccaagtgga gagagtcctg gaccggggaa
    10561 cgcaccgctg ggtgttattt cgcccattgg gcttggaagt tcatctggag atcaaccttc
    10621 ccaactacgt ctttcgcaac ctgggactgg gggaggggaa ggagatccag gtggccatgc
    10681 ggcgggagtc cttctgggtg attcccacga aatagcggtc agcactcagc catcagccaa
    10741 aagctgtagg ggcgggtttg aaacccgccc cgatttttta cctctcactc ctcattcttc
    10801 tcactcttct cctcatccag gctgaagtag gtcaggtaca ccccctggac cccttcgatt
    10861 ccctggaggc tctccatgcg ggccttcagc tctccggcat tctccccctc ggccaggaaa
    10921 acgacctttt cctctttcac tccgtggagt tcaatccccc gagcctccag ttccttcagg
    10981 atggagtctt tgaaggagac atcgatcagc aggaatccgc tgctcacgat cattttccga
    11041 acctcagggc aagggtggac tttcggcttg ggcaggcctc gacgcatcgc ccgcacagga
    11101 tgcaggaaga atcgcggatc ttttcccgag cggggggaga tagaa
//
LOCUS       DASPRR010000028         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2266541, whole genome shotgun sequence.
ACCESSION   DASPRR010000028 DASPRR010000000
VERSION     DASPRR010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266541"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(602..1522)
                     /locus_tag="VEL68_01005"
     CDS             complement(602..1522)
                     /locus_tag="VEL68_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ34575.1"
                     /translation="MKGYILYRLFQAVIVIWGISLITFSLLHMFRDPVLILLPPEATK
                     EEVETLRKEMGLDRPIAVRYVKFLGGMVRGDFGRSFVAQRPALGLVMERMPMTLWLAA
                     SGLLLAVIISIPLGVIAAVRRNRMADHSASIFAVLGQAMPLFWLDIMLIIIFAVKLRL
                     LPASGAGSYKHVLLPAAGIAVSLLPILMRLTRSRMIEILSQDYIRTARAKGLRESTVL
                     FRHALRNALIPVVTILGFQFGMLLGGAVITETIFAWPGVASLVVESIFNSDFPVVQAA
                     VAVFALLIVFANLITDITVAWLDPKTRFGT"
     gene            complement(1614..3149)
                     /locus_tag="VEL68_01010"
     CDS             complement(1614..3149)
                     /locus_tag="VEL68_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34576.1"
                     /translation="MREIRKRIRVAIFLMLAGLLIFPGPSSAAPQGSMTVALSSDISS
                     LDTQYHNIRVNYIVGWNLYDNLVYRDQKTLKIGPHLAESWKLIDDNTWEFKLRRGIKF
                     GDGEPFTAESVKFTIERGQDPKCPQRPYVSWIKEVKIIDDYTLRLISANPYPVALERL
                     ANFQMLPAKWLKEKGTDYFTTHANGTGPYRLKEWKRGVHVILEVKDNYWKGAPAIKTI
                     MFRPIKEISTQISELLTGGIDIVRDIPPDQISLVENSGIARVSKAPTLRQVYLVMDAD
                     GRAGRSPVQNLKVRQAISHAIDIDTLIKTVMNGNATRTAACLNPAHFGYDKSLENPYP
                     YNPEKAKQLLKEASFPFDQPLRLVTYTGSIQNPRALIEAVAGFLNKVGIKTNVNVYTD
                     IGTWDRLGREGKQNDLSLHSWGGGGVYDADAVYYNYFRKGEAFCYGSSPEMDRWLDQA
                     RSTMNPEVRKKLYFQAGKYINDHALAFSFWSQYTILGLNKRIIYEAPPDEFLRTFEAK
                     LKE"
     gene            complement(3288..>4212)
                     /locus_tag="VEL68_01015"
     CDS             complement(3288..>4212)
                     /locus_tag="VEL68_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34577.1"
                     /translation="KDDYWKGAPPVKTIVFRPIKEISTQISELLTGGIDIVRDVPPDQ
                     IPLIESSPVARISKAPTLRVVYLVMDADGRAGKSQVQNLKVRQAINYAIDVDTIIKTV
                     MTGNATRTAAVLNPQHFGYDKRLENPYPYNPEKARQLLKEANFNFDQPLKLTTYTGSI
                     QNPRALIEAVAGYLGKVGIKTNLNVYSDIGMWDRLSREGKHNDMSIHSWGGGGLYDAD
                     AVYYSYFRSGEAYCFGSSPEMDQWLDEARTTMNPELRKKLYYQAGKFINDQALAVSFY
                     AQQAILGINKRVIYEAPPDEFLRTFEAKLKE"
BASE COUNT          873 a         1170 c         1243 g          926 t
ORIGIN      
        1 gggagatgaa aggggacagc agggccatgg ccagcatcag gaaaaccatg accaggccca
       61 ccatccctgt cttggcatgg cccagacggc ggaaaaaata tcggtagccg cttcttcctg
      121 tcatggttga atgcccacgt aatacgaaaa ataaattttc cctcccccag cgggaggggg
      181 tgaaggggag ggggccaaag ttgaatcacc cccacccgcg ccctcccccg tcaaggggga
      241 gggaatttcc ctatttcgcc atgcgtatcc tctctcccag cgggagagag aagctgtgaa
      301 aaaaatggat gaacacggcg aaggcctcgg ttgttgcccg gggaaggagg gaaattcgca
      361 gcgccaaaac ccggcgggag atgcatggcc gtgaagcgct tcctcgattt ccgcaaagcg
      421 acgccggcta cttgcgaaaa cttttgcaag tacgaaagtg tgatcgtttc ggcttccatg
      481 atttaaattc cgagtgggcc acggagaaat cggtgcctgt ttcggtactg cgaatttccc
      541 ctcttccctg ggcatcccca ggccacccag atttccactg gccaaatttc ttcgcggcct
      601 cctatgttcc aaacctcgtc tttgggtcga gccaggcaac agttatatcg gtgattaaat
      661 tggcgaacac gatcaagagg gcgaagacgg ccaccgctgc ctggacaacg ggaaagtcgg
      721 agttgaagat cgattccacc accaggcttg ccacgcccgg ccaggcgaag atcgtttcgg
      781 tgattaccgc tcctcccaaa agcattccga actgaaaacc gaggatggtg accaccggga
      841 tcagtgcgtt gcgcagcgcg tggcggaaga ggactgtact ctcccgcagg cccttggcgc
      901 gtgcggtgcg gatgtaatcc tgggagagga tttcgatcat cctcgaccgg gtcaggcgca
      961 tgagaatggg aaggagagat accgcgatcc ccgcggcggg aaggaggacg tgtttgtagc
     1021 tcccagcccc cgacgcgggc aagaggcgga gtttcaccgc aaagatgatg atgagcatga
     1081 tgtccagcca gaagaggggc atggcttgtc ccaagaccgc gaagattgag gctgagtggt
     1141 ccgccatgcg gttgcgtctc accgccgcga tcacccccag ggggatggag atgatgaccg
     1201 cgaggagaag gcctgaggcc gccagccaga gggtcatggg catgcgttcc atgaccaggc
     1261 ccagggccgg gcgctgggcg acgaaagacc ggccgaaatc cccccgcacc atcccgccga
     1321 gaaacttgac gtaccgcacg gcgattggcc ggtccaggcc catctccttg cgcagggttt
     1381 ccacctcttc cttggtggct tccgggggga ggagaatgag aaccgggtcg cggaacatgt
     1441 gcaggaggga gaaggtgatt aaactgatcc cccagatcac gatcacagcc tggaataatc
     1501 tgtagaggat gtaacctttc ataaggtaaa gcagctcgta gctgatagct catagctcat
     1561 aggaaaaccc ctgtaagatg cgaactatga gctatgaacc atgagcccaa ttgctattct
     1621 ttcaattttg cctcaaaggt cctcaggaat tcatcgggcg gggcctcgta gataatccgt
     1681 ttgttgaggc ccaggatggt gtattgcgac cagaaggaga acgccagggc gtggtcgttg
     1741 atgtatttcc cagcctggaa atagagcttc ttccttacct cggggttcat ggtggagcga
     1801 gcctggtcca gccatctatc catttccggg gaagacccgt agcagaaagc ctctcccttg
     1861 cggaaatagt tgtaatagac cgcatctgca tcgtagaccc cgccgcctcc ccaactgtgc
     1921 aggctcaaat cgttctgttt cccttcccgg cccagccggt cccaggtgcc gatgtccgtg
     1981 tagacgttca cgttggtctt gatcccgacc ttgttaagga atccggccac ggcctcgatc
     2041 agggcccgcg ggttctggat gcttccggtg taggtgacca ggcgcagggg ctgatcgaaa
     2101 ggaaagctgg cctccttcag gagctgcttg gccttttctg gattataggg gtatgggttc
     2161 tccagggact tgtcataccc gaagtgggcc gggttcaggc acgccgcggt gcgcgtggca
     2221 tttccgttca tgaccgtttt aatcaaggtg tcaatgtcga tagcatggct gatggcctgc
     2281 cggaccttca agttctggac cggggatctt cccgcccggc cgtcggcgtc catgaccagg
     2341 tagacctgcc gcagggtggg ggccttggag acccgggcga tcccggaatt ctccaccagg
     2401 gagatctggt cgggaggaat atcgcggacg atgtcgatcc ccccggtcag gagctcggag
     2461 atctgggtgg agatttcctt gatggggcgg aacatgatcg tcttgatggc cggggctccc
     2521 ttccagtaat tgtccttgac ctccaggatc acgtgcaccc cccgcttcca ttctttcagc
     2581 cggtagggcc ccgttccgtt ggcatgggtg gtaaagtagt cggttccctt ctccttcagc
     2641 cattttgccg ggagcatctg aaaattcgcc agccgttcga gcgccacagg ataggggttg
     2701 gcgctgatca ggcggagggt gtaatcatcg atgatcttca cttcctttat ccaggacaca
     2761 taaggccgct gagggcactt ggggtcctgg ccacgctcga tggtgaactt cacgctctcg
     2821 gccgtaaaag gctccccgtc tccgaacttg atccccctgc gcagcttgaa ttcccaggtg
     2881 ttgtcgtcga tgagcttcca ggactcggcc aggtgggggc cgatcttcaa agtcttctgg
     2941 tcccggtaca ccaggttgtc atagagattc caccccacga tgtaattgac ccggatgttg
     3001 tggtactggg tgtccaggct gctgatgtcg ctcgaaaggg cgaccgtcat gcttccctga
     3061 ggagctgcgc tggaagggcc ggggaaaatg agaagccccg ccaacataag aaaaattgcg
     3121 acccggatcc tcttcctgat ttctcgcatg tttactgcct cctacctttc aaaaaacagg
     3181 gggaaaaggg gaaaaggcga agaggcgaaa aaagttaaaa ccgatccccc atcacagttt
     3241 ccggtttttc ccttttcgcc tttccccctt ttcccccctt cgcccatcta ttctttcagc
     3301 ttggcttcga aggtcctcag gaattcatcc gggggggctt cgtagatcac acgcttattg
     3361 atgcccagga tggcctgctg agcataaaaa gagaccgcca gagcttggtc gtttatgaat
     3421 ttgcccgcct ggtaataaag ctttttacgc aattccgggt tcatcgtcgt gcgcgcttcg
     3481 tccagccatt ggtccatctc gggcgaggac ccaaagcagt aagcctcccc gctgcggaaa
     3541 tagctgtagt aaacggcgtc ggcatcatac aggccccctc cgccccagct atgaatgctc
     3601 atgtcgttat gcttcccctc gcggctcagg cggtcccaca tgccgatatc ggagtacaca
     3661 ttcaggttcg tcttgatccc cactttgccc aggtatccgg ccactgcctc gatcagagcc
     3721 cggggatttt gaatgctccc ggtataggtc gtcaatttca gcggctgatc aaagttgaaa
     3781 ttggcctcct tgagaagctg ccgggctttt tccgggttgt aggggtaggg gttttccaaa
     3841 cgcttgtcgt aaccgaaatg ctggggattc aggacggcag cagtgcgggt tgcatttccg
     3901 gtcatgaccg ttttaataat cgtgtccacg tcgattgcgt agttgatcgc ctggcggacc
     3961 ttcaggttct ggacctggga tttccccgcc cgtccgtcgg catccatgac caggtagacg
     4021 acccgcaggg taggggcctt ggagattctg gccacggggg aactttcaat gagggggatc
     4081 tgatccggcg gaacgtcgcg gacgatgtcg atccccccgg tgagaagctc ggagatctgg
     4141 gtggagatct ctttgatcgg gcggaagacg atggtcttta cgggaggggc tcccttccag
     4201 taatcgtcct tg
//
LOCUS       DASPRR010000029        32490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1617814, whole genome shotgun sequence.
ACCESSION   DASPRR010000029 DASPRR010000000
VERSION     DASPRR010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 32490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32490)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32490
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617814"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VEL68_01020"
     CDS             complement(<1..866)
                     /locus_tag="VEL68_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ34578.1"
                     /translation="MESVVVPDDFPSILAGTAAVKRLELHAKVTVYTKKASTPEELIQ
                     RLQGAIVAVNIRAYSKFDEPLLKACPDLRMIAVLGVGTDNVDLGAASRLGIRVTNTPG
                     FSAVSVAEHTLALMLAAARKIPGHEQELRAGRWTRFPMTQLHGKRMGIIGFGNIGRQF
                     AGLARGIGMKVWAWTFNPSPQRAEEAGIQFVEFDQLLSQSDVIAVTIKASEKTRGLIS
                     RQALAKVKPSCILVNTARASIVDTGAMIEALRTGRLAAAALDVYDREPLPADDPILSL
                     PNVVISPHNAGMT"
     gene            complement(908..1444)
                     /locus_tag="VEL68_01025"
     CDS             complement(908..1444)
                     /locus_tag="VEL68_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34579.1"
                     /translation="MAEIKSTVELALERTKGLRLSHEEMEKMKEEELQSKAIGLVNRF
                     LEVDFHLRDVEKELAKFDPQQRKHLEQLFLQHLIEAMNLDRDNDLILQGLESLIPKSA
                     WTVKKIKDLLQRYQLRKKDESLKTKTELLAGLERLGISGSAVHPKVEGSPEWEQAVST
                     FKPGFEEELKKLKKGILH"
     gene            1672..2100
                     /locus_tag="VEL68_01030"
     CDS             1672..2100
                     /locus_tag="VEL68_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXZ34580.1"
                     /translation="MPPKDFDQFEDQVYRLMSDMFKDAKPLGYQPDRCFSPPMDIYET
                     EENLVVVMEIAGMKPENIQVTFEKDILSIRGFRNEPSSPPKTRLHQVEIDYGRFQRTL
                     RIPFPLKSDDFKAIYRLGFLVVTVPKAKRPVSLNVEVNVR"
     gene            2175..4520
                     /gene="lon"
                     /locus_tag="VEL68_01035"
     CDS             2175..4520
                     /gene="lon"
                     /locus_tag="VEL68_01035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXZ34581.1"
                     /translation="MEIPENLPILPIRESVLFPKMLLPLMVSQERHVKLIDAALLANK
                     MIGIAAIKKKGVTEVKPEDLYEVGTAAYILKMLKMPNDSLRLLIQGISRIRVGAFTQQ
                     EPFLRAQVTTLAEQGEKTTETQALMAGVKGIFQKFVELAPNLPADLAIMAMNVEEPGV
                     LADLIASTLNLSLEDKQAILESMEVKARLEKVNLLLNKELQVLELGSKIQTQVREGID
                     KSQREYFLREQLKAIQKELGEKDERTAEIEELRERIAKAKLPPEALKEAERELDRLAK
                     MPPSAPEYTVARTYLDWLIELPWAVATEDNLEIQPAQKVLDEDHYDLEKVKKRILEYL
                     SVRKLKADMKGPILCFVGPPGTGKTSVGKSIAKALGRKFIRMSLGGVRDEAEVRGHRR
                     TYVGALPGRIIQGIRKAGSNNPVFMLDEIDKLGMDFRGDPSSALLEVLDPEQNYSFSD
                     HYLEVPFDLSKVMFITTANILDPIPPALRDRMEVLDLPGYTEEEKLRIAKDFLVPKQT
                     QAHGLTDQTIQFQEDALRRIALEYTREAGVRNLEREIANICRGVARKVAEGAQGLTIV
                     DAESVPTYLGPIKFFSEVAERTSEPGVATGLAWTPTGGDILFVEATRMKGRKGFSLTG
                     QLGEVMKESAQAALSYVRAKAKALNIPDNFFDSSDIHIHVPAGAIPKDGPSAGVTIFT
                     AITSLLTARPVRSDVAMTGEITLRGLVLPVGGIKEKVLAARRAGITTLILPKKNEKDL
                     EEVPEQVKKDLKFHFVQRMDEVIHIALKPKMKPAQKPKSKK"
     gene            complement(4789..5988)
                     /gene="ablA"
                     /locus_tag="VEL68_01040"
     CDS             complement(4789..5988)
                     /gene="ablA"
                     /locus_tag="VEL68_01040"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HXZ34582.1"
                     /translation="MSVSGSTVLPREPIRSIRLAEAKIPVEEEYQPNPWKDATDEEWE
                     DWRWQLRNRITKAEQLRLLLNLNDEEVAAMDASKGRMATAITPYFATLMSRTDPNCPI
                     RRQALPTLQEIHFSPHDLLDPCAEDENSPVHGLVHRYPDRVLLLVTDKCAVYCRYCTR
                     RRMVGSSEKCITEEEMDEAINYIQATKKVRDVLISGGDPLLLEDDHLERILTKLRKIP
                     HVEIVRIGTRVPVTLPQRITPALTNMLRKYHPLWMSIHFSHPKEITKEVRRSCGLLSD
                     AGIPLGSQTVLLRGINDKPSIMKRLMHELLKTRVRPYYIYQCDLAMGTEHFRTPIAVG
                     INIIEKLRGHTTGYAVPSFVIDAPGGGGKIPVGPTYLISQDKGRMVLRNYQGKVFEYQ
                     EPGDEDS"
     gene            complement(5998..6282)
                     /locus_tag="VEL68_01045"
     CDS             complement(5998..6282)
                     /locus_tag="VEL68_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXZ34583.1"
                     /translation="MRTSDILKKLNIPRHKLYYLEQKGYIKPKRIPMGELESREYSEE
                     EFRKLELIWKYLQSGFKHKIAYEKALEELQSPKLRLDSQAQKPGPTESKT"
     gene            complement(6579..7742)
                     /locus_tag="VEL68_01050"
     CDS             complement(6579..7742)
                     /locus_tag="VEL68_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ34584.1"
                     /translation="MTSRERILTALSHREPDRVPYDLGSLGPSGMSIQAYRNLLKYIH
                     SGERAEVGDMASQRAKLSEDFLQRFRVDTRSFRVRPHGAWSLKMQEEGGYSFFYDEWG
                     IGRKLAKDRGMHFFIFHQPLSEVETSDLPRFPWPDPLDPNRLAGVEKEVLAVRRQVDP
                     AFVLGSSFSSGLLQFGAQLEGHERFFTDLVLDPLRTEWLLDKLLHLKLSFYQNVLDRM
                     GKWIDIVCEMDDFGHQHSLWISFEMFRKLIKPRYVELFSFIKKRYGKKILLHSDGAIY
                     PLIPDIIEMGVEILNPIQLGARDMGDTRKLKKDFGDALTFWGGGVDIQHTLPFSTPQQ
                     VEDEVKRRIEDLAPGGGFVFAATQAIQPETPPENIIAMWNALQRYGIYPGAQD"
     gene            complement(7843..9510)
                     /gene="ilvD"
                     /locus_tag="VEL68_01055"
     CDS             complement(7843..9510)
                     /gene="ilvD"
                     /locus_tag="VEL68_01055"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXZ34585.1"
                     /translation="MRSDLMKKGLEKAPHRSLFKAMGLTEEEIRRPMVGIANSANELI
                     PGHIHLDRIVEAVKAGIRMAGGTPLEFSTIGVCDGIAMNHEGMKYSLASRELIADSVE
                     IMAMAHPFDAMVMVPNCDKIVPGMLMAALRVNIPSIVISGGPMMTGKVADKEVDLISV
                     FEGVGRVARGEWNRARLKELEDCACPGCGSCAGMFTANSMNCMSEAVGLALPGNGTVP
                     AVNAARIRLAKIAGMKVMELWTRNIKPRDIATAAAFKNAMAADMALGCSTNTVLHLPA
                     IAHEAKIDLNLDLFNKVSDRTPHLCFLSPAGPHHLHDLDAAGGVQAVMAELSKLGVID
                     RKVMTATGKTLGENLKGVKVKDYKVIRPLTNPYHKEGGLAILRGNLAPGGAVVKQSAV
                     AAEMLVNEGRAIVFESEPEAMGAILRGKVKPGHIVIVRYEGPKGGPGMQEMLSPTSAI
                     TGMGLDKDVALITDGRFSGGSRGAAIGHVSPEAAEGGPIALVRNGDRIRIDIPKKKLT
                     LLVNEEELKRRKKSWKPRKPKITSGYAARYAQLVTSAGTGAVFKDRL"
     gene            9915..11336
                     /locus_tag="VEL68_01060"
     CDS             9915..11336
                     /locus_tag="VEL68_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXZ34586.1"
                     /translation="MVSGKTQLATLTHRGKYRGARPHPEFTLREKQIPLFASSANGNG
                     QAAFRDPAFTENKNLPVHRWVPWIAGYSASFVDDAISSYLPRGRTGMVLDPFCGVGTT
                     LVQSVIRGHDAIGFEINPYAALATLAKLRALTIDESDFTKAITSLRKNSIGWKTNSPP
                     EDIKTPPFKSRLPFFSPKVERQILHALAFIKAIESQPIGDLFRVAFGAVMVSVSNYSY
                     EPSLGSRPAAGKPLVDDAEVGQILLNKLSQMRTDIIWVHQEIKGKSLGTGRVINADYF
                     KVNGQLGNGSIDLMITSPPYMNNYHYVRNTRPQLYWLNFISSPTEQKHLETNNFGQYW
                     QTVRDASPVSLTFEHKELEKILKKLRETRIDKGAYGGPGWANYVASYFNDSYRFMSSL
                     KYLLARGGVGVIVIGNSIIQGIDIKTEKILSEIGEIVGLIPEGVHCIREKRVGASITQ
                     SSVRYGELNRAVLSESAVIIRKR"
     gene            11341..11940
                     /locus_tag="VEL68_01065"
     CDS             11341..11940
                     /locus_tag="VEL68_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HXZ34587.1"
                     /translation="MGNEPYNPLDKLRLGESVTKALLARPVVPLPIPEPFAGAGIYAI
                     YYAGKFSPYHKIAEKNINNQWAAPIYVGKAVPPGARKGGYGLDQSPGDVLYRRLREHA
                     ESIQQAENLLISDFRCRHLIVDDIWIPLGESLLISMFFPLWNQVLDGFGNHDPGAGRY
                     NQQRSPWDVIHPGRPWAAKLRPNSRSEEEILRSISEFRP"
     gene            12289..15279
                     /locus_tag="VEL68_01070"
     CDS             12289..15279
                     /locus_tag="VEL68_01070"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXZ34588.1"
                     /translation="MGFHFSASKKSFGTFTAWLPASDARPVASSSLVADPPGPQDETI
                     LQPWTITALRLSPAQTVELLCASMGKQILSPGIVVGKDVFFWAQALRFAASLVARQQF
                     LPGVAEEDGGVSAKWKPVIHGPDGERFAVLAGAMPAVSRAVMGTGKSLQTTSDAPAAP
                     SPVSLLSFFIEKVVDHLVRSAGLWLNTGGLRGDSKDFESSHDQWLHSLRAGDGTMAGS
                     PTEWAELTGQIQEWQRPLAISAASPFRLCFRLEEPNGNEDQKEKAGLRMASWYVRYLL
                     QAAQDPSLLIPAEEAWKAKDRAGKKGRSSSPLLQDGFEPREYLLLSLGQASGIWPRIE
                     ASLAKPAPAGYELDAAGAHDFLTQKALALRQAGYGVMLPAWWTGLGTKDRLTARASVQ
                     APRMQGTGGLSLDEIVKFDWEVALGGEGISFEELQALARLKVPLVKLRGQWVQVNAEE
                     IEAALALWKKKGQATAREIVQMALGGSRISGQIPIDGLRATGWISDLLAELEGKTPFE
                     ELPAPAEFQGTLRPYQVRGYSWLAFLRKWGLGACLADDMGLGKTIQALALIQRDWHAF
                     VKRPVLLVCPTTVVNNWRKETARFTPDLPVMVHHGILRMRGEDFRQEALKNAVVISSY
                     ALLHRDFETLKEIPWSGIILDEAQNIKNPETKQARAARGFQADYRLALTGTPVENNVG
                     DLWSIMEFLNPGFLGTQAAFKRGFFIPIQAARDPQAMERLKRLTGPFILRRLKTDKTV
                     IADLPEKMEMKVFCSLTKEQASLYEAVVKETMKTLDATEGIQRKGAVLATLSKLKQVC
                     NHPAQFLGDNSPLPGRSGKLARLAEMVEEMMAVGDRALIFSQFAEMGEILRRYLQETF
                     GQEVLFLHGAVPKGQRDRMVERFQGAQDGPSIFILSLKAGGTGLNLTRANHVFHFDRW
                     WNPAVENQATDRAFRIGQTKNVQVHKFLCAGTLEEKIDEMIERKKGIAEKVIGTGEGW
                     LTELSTAELRDLFSLRKEAMGTGE"
     gene            15281..16195
                     /locus_tag="VEL68_01075"
     CDS             15281..16195
                     /locus_tag="VEL68_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HXZ34589.1"
                     /translation="MRRWDHYRFFEPSVPRRAKGGIRAQSKRGTFGESWWAKRWISVL
                     ESFDIGARMGRGRNYARSGQVLSIDIEKGAVKAKVQGSRPTPYRVAVEIKSISYANWE
                     KLVKALSHQAIFVAKLLAGEMPQDIEKVFQAAGLSLFPDKSKDLKTECSCPDWSNPCK
                     HIAAVYYLLGEEFDRDPFLIFKLRGLQREELIQRLGNREKRSDRKKRKSKPPPAHDEE
                     KKIPISESLPADGSLFWTGGALPEDFFGEVQVPPVSAALLKRLGKFPFWRGKERFFDA
                     LEPVYTRASTRGLAVFLGEKNGPEVPSK"
     gene            complement(16371..17150)
                     /locus_tag="VEL68_01080"
     CDS             complement(16371..17150)
                     /locus_tag="VEL68_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXZ34590.1"
                     /translation="MIVAGMDIGSITTETVILRDRNILTSVIMPTGANSRNAAERCLS
                     AALEKTSLNQGDLTVIVTTGYGRASFPGATKRITEITCHARGAFFVHPQTRTVIDIGG
                     QDSKVIRLDAQGRNVDFQMNDKCAAGTGRFLEVMAHALEVKVEDLGRLSLIAPRTIKI
                     SSMCTVFAESEVVSLIADNQPKEVIIRGLHDSIADRILGMVNRLGIEEEVTLTGGVAK
                     NEGVVRALEDRLGVRMFVPPEPQIIGALGAALLARDLLPRP"
     gene            17324..18475
                     /locus_tag="VEL68_01085"
     CDS             17324..18475
                     /locus_tag="VEL68_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ34591.1"
                     /translation="MDFLLSEEQKMVQKLARDFAEKELKPRAVETEAARQLPLSILKA
                     MADLGMMGMNVPAEYGGAQTGVVSYSLAMTEIGKGCASTAVTMAVTNMVAEVIHAFGR
                     EEHRRNHIPKICGGEYPSGAFALTESTAGSDASSMKSTAERSGDYYLLNGSKTFITSG
                     DFAGIFVVWAKTAKTTGAKGISAFLVEQGTPGFKVGRNEEKMGQRGSPTNEISIEDCR
                     LPRDSILGREGDGFRIAMMALDGGRIGIGSLAVGIGLAAMEYATEYAKNRIQFGKPIS
                     SFQALQWMIADSFTELEAARLLVLRAAHRKERGLHFTREASMGKLLATETANRVCYKA
                     LQMLGGYGYMKEYPLERYTRDCRVTTIYEGTSEIQRLVIAREILGGDYV"
     gene            complement(18549..20450)
                     /locus_tag="VEL68_01090"
     CDS             complement(18549..20450)
                     /locus_tag="VEL68_01090"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXZ34592.1"
                     /translation="MLDFMRKHAQSWMIKAALGAVVVVFIFWGIWAPREGRQRELIKI
                     GPYIITVTEARNYYQNMRDRYQSIYGERFNEDMAKKLNLKERAVKDLVNRILLLQEAQ
                     RLGLKVAEEEMEASIHSIPAFQKDGIFDQATYSRALQRARLNPKEFEANQRDLLLINK
                     LQGLILSSIRVSEREVQEAYRQSFERINLEMISLNPADFKEVSITPEELKEFFSKHKE
                     DFKVPTKVKIRYLLFEPKDFFKQIPVSFKEIEDYYQNNKEKFGRPKRVKVRHILIKAE
                     AQDKEGSAKARQKAESVREEAAQGKDFAQLAKKYSEDPGTKDRGGEIGFISKGMVVPE
                     FEQAAFSMKAGEISSVIQTPYGFHILKADEIQEASTQPMEKVKDQIEVLLKNRQAREL
                     AYDMADQAFARASKDKTLDKFAEEKKLTVKETLLFSAEDKIDLDPKLKTSALSMGKGD
                     VSPPLRVGETFAVMQAVEKQESRTPDLKEVEGKVTEALRKEKQKDMALAKAKELLEKL
                     KKGSAFKALASQEGLKIEETGFFPRASAPPKVSPSEDMLKALTALSVRNPYPESPIFQ
                     DGKYSILYLKEVKEIDQAQFDSQKENYRRGLLMQKQETILGSWIENLLEKAKAKGDYK
                     ELQPVNEAI"
     gene            20709..21746
                     /locus_tag="VEL68_01095"
     CDS             20709..21746
                     /locus_tag="VEL68_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXZ34593.1"
                     /translation="MIFDFVLGLFSNDLAIDLGTATTLVYVKGKGIVLCEPSVVAVRK
                     DSKGGRKILAVGKEAKKMLGRTPGNIVAIRPMKDGVIADFEITESMLRYFIAQVHKGR
                     TLVRPRIIVSVPSGITEVEKRAVRESAQSAGAREVYLIEEPMAAAIGASLPVNEPSGN
                     MIVDIGGGTCEVAVISLAGIVYSQSVRVGGDKMDEAITNFVKRKYNLLIGERTAELIK
                     IQIGTAYPDEDVRTMEIKGRDLVAGNPKVLQINSEEVREALTEPINAILEAVKTTLER
                     TPPELAADIVDKGIVLAGGGSLLRNLDVLIREETGLPVTITEDPLSAVVLGSGKALDE
                     LNLLKEVTIKT"
     gene            21880..22692
                     /gene="mreC"
                     /locus_tag="VEL68_01100"
     CDS             21880..22692
                     /gene="mreC"
                     /locus_tag="VEL68_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXZ34594.1"
                     /translation="MLNLLKRFRLLFTALLFLFLALVLMTIYAKEGRETSLIQKVLLQ
                     ISFPFQHGAQKTFLWFKEVGEDYLFLSRVRQENKDLKRVLSTLQEENNRLREALQTDE
                     RLKKLSVWQSQYPHKSRVAQIYARGPSSWFKTLLINKGENDGVAKDMAVATSEGVVGR
                     VIEVSPGTAKVLLVTDANSAVDVIVQRSRVQGIMEGTIDEVCILKYIQKNEEVQVGDV
                     VVTSGQGGIFPKGLVMGKVSEVDRKRHGIFQYIEVTPSVDLAKLEEVLVLEK"
     gene            22689..23192
                     /locus_tag="VEL68_01105"
     CDS             22689..23192
                     /locus_tag="VEL68_01105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ34595.1"
                     /translation="MIETLKIRRIQPQDRPSLKALLHAQSHFRPEEIRVALELIDLAL
                     AQPGEEDYVILCAEEREGEIRGYICYGKAPLTEAVYDIYWIVVHPAYWSQGTGSSLLR
                     QAEGELAQRQARLLLIETSSTPPYRIPRAFYGKHGYREQARVRDYYRPDDHKIIYSKN
                     LKGRNET"
     gene            23308..24912
                     /locus_tag="VEL68_01110"
     CDS             23308..24912
                     /locus_tag="VEL68_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ34596.1"
                     /translation="MTGIAKVWEKIKEIHRRRAPFFRRPLGLQTRITLLFAVVVVSVI
                     VLFSYLEFRLAEHSQRELWRERTIYVTREVDAKIYSSKDLENAAFLEEEIANWMYARP
                     SIRSIDIFVFQKNTYKIMASSWEGKDLGISYQSLDSLKRDMVLSTLIKRDDHPHWEIL
                     APLHAGRKIVGGIRIVTSLEELETFLANKRTRTILFTLISVAILILILAVFFSRAIHR
                     PIQQLVRAMSEAESGKLNVAVPVRSQDELGLLSVHFNRMLSRISQFNEELTQRIEAAT
                     RELAQRNEELRLANESLFQAQRQLVQAEKLSALGHVAATMAHEIGTPLNSISGYIQLM
                     LAEETGSELMTKRLVIIETQLDRLTQKIRNLLHSTRQPEPQLQAVDVNHVLETVITLT
                     QPGMSIRAVQLVRHLGPDLPRISGDPSLLQQVFLNLVTNALDAMPQGGVLTLITAPPA
                     ASGRNGYFVEVVVEDTGTGMSPEVKKKALEPFFTTKSPGKGAGLGLSICEDIIRSHHG
                     KMEIESQEGKGSTLRIQLPVFSPEGV"
     gene            24915..26279
                     /locus_tag="VEL68_01115"
     CDS             24915..26279
                     /locus_tag="VEL68_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ34597.1"
                     /translation="MASPVRILVVDDDEITCNLLEEVLSKEGYAVDKALNGQEAIEKG
                     EKESYGVVLSDIRMLDVDGMEVLRAYRQRSPETIIIMMTAFGSIETAIRAIKEGAYDY
                     VSKPFKLDEIKLTIQRSLEQKRLVEENIHYRQELITKYKLENIVGRSPQMLQVYKTIA
                     RVAESRSTVLITGESGTGKELIARAIHFNSPRSPRPFVAVDCSSLAESLLESELFGHV
                     RGAFTGAVTTKKGLFEEADRGTCFLDEISNISLAIQAKLLRVLQEHEIKRVGGTETIK
                     IDVRMVAATNQNLEELVAEKRFREDLFYRLNVVSIHLPSLRERPEDIPLLADHFLNKY
                     ATQNNKPVCRISPDAMDVLTRYRWPGNVRELENVIERAATLSNTNLIIPADLPRRLQV
                     ELSQNAISSLPSSLPLSELEKLYIQKVLEETGGNKKKAADILGIDRRTLYRMATRLGL
                     PIKG"
     gene            26556..26873
                     /locus_tag="VEL68_01120"
     CDS             26556..26873
                     /locus_tag="VEL68_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34598.1"
                     /translation="MKYNPLQRRLGSWSLVVIILLVFAGCSSLKSENEKLKEEIVDVS
                     AENEKLRKELNTLKSENSKMHMRVAQLHMEIAALYQEIQNMQKDLDALKAQLKGGGRN
                     KKS"
     gene            26941..27147
                     /locus_tag="VEL68_01125"
     CDS             26941..27147
                     /locus_tag="VEL68_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34599.1"
                     /translation="MKKLIALLVCVLFAMTLMVAACAKKEEPAKPAEPAKKEEVKPAE
                     PAKPAEAAKPAEPAKPAEKAPEKK"
     gene            27211..27627
                     /locus_tag="VEL68_01130"
     CDS             27211..27627
                     /locus_tag="VEL68_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34600.1"
                     /translation="MKRILILMVALLTIVAFAGGVMAQAPAKTEKPAAPAPEKAAPAP
                     APKPAAEKPKALKATGAVAAYEAGKMIAVKVKDKEMKFDIAADAKVQGEVKEGAKVTV
                     TYKKDMDKMVATSISGPKPKKEAPPKKAPEKAPEKK"
     gene            27980..30391
                     /locus_tag="VEL68_01135"
     CDS             27980..30391
                     /locus_tag="VEL68_01135"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HXZ34601.1"
                     /translation="MRSRKNNKNRLLILITGIFLLLLATPLLASEAELEIPVLTDSQN
                     TLLMVGFLICFLGMAFGAYEYVKVKAFPAHKSMLDVANVIFQTCKTYLLQQGKLLIIL
                     EIFIGVCIAYYFGFLQHMDAGGVILILIWSVVGILGSYSVAWFGIRMNTLANSRMAFA
                     SLEGKPIKLLDIPLRAGMSIGVLLVCVELIMMLIILVFMPRSTAGACFIGFAIGESLG
                     ASALRICGGIFTKIADIGSDLMKIVFKIKEDDPRNPGVIADCTGDNAGDSVGPTADGF
                     ETYGVTGVALISFIVLGVFEQFRAELITWIFVMRILMILTSIVAFQFNKAVTTARFAG
                     KTKVDFELSLTMLVWFTSILSIIVTFAASYWQIGHLPNNLWFNLSIIISCGTIAAALI
                     PELVKVFTSSKSIHCREAVTSAREGGASLDILSGFVAGNFSAFWLGMAIFLLMSVAYF
                     ASLNGLDKFMEYSSIFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSIYELSLIETIP
                     NVETEIQSQFGFKPNFENAKHFLEENDSCGNTFKATAKPVLIGTAVVGATTMIFSLIL
                     LLRKTLGIQPETILNLLNPFTLLGFLCGGAVIYWFTGASTQAVTTGAYRAVQYIKKNI
                     KLDDSASLSASTEKSKEVVKICTQYAQKGMFNIFITIFCFALGFAFFSAPGSSNAPAS
                     FFISYLISIAVFGLYQAIFMANAGGCWDNAKKVVEVDLREKGTPLHAATVIGDTVGDP
                     FKDTSSVALNPIIKFTTLFGLLAMEIAISPMMKGIAHYVGVVFFLIALVFVARSFYGM
                     RIPKE"
     gene            30576..30974
                     /locus_tag="VEL68_01140"
     CDS             30576..30974
                     /locus_tag="VEL68_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HXZ34602.1"
                     /translation="MHIKKLYFSVLLLLPATLLLAPGGMALAQTITPAHEQEFTDAKN
                     AIDAAQKAQAEKYASESLKQAQDLLVAAGNARSSKDAVKFTQNSRLARAYAELARATA
                     ELKTEEDKLAATQDELQKARAEIESLKKAQ"
     gene            30991..31191
                     /locus_tag="VEL68_01145"
     CDS             30991..31191
                     /locus_tag="VEL68_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34603.1"
                     /translation="MKPSVSHRIQILFLLFLGYLLVAYQNLNRSQVTSEGLGPSQPVA
                     NDATEAGRALNRRVEIAVQTGE"
     gene            31280..32290
                     /gene="galT"
                     /locus_tag="VEL68_01150"
     CDS             31280..32290
                     /gene="galT"
                     /locus_tag="VEL68_01150"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HXZ34604.1"
                     /translation="MPELRQDPATKQWVIIATERAKRPHDFSRPPVQVSVEEYKADCP
                     FCLGNESLTPPEIMAYRKGGPANGPGWWVRAFPNKFSALVPEGSLSRKEEEGIFRKMD
                     GVGQHEVIVECPQHNLCIPLMADKQVEEIILAYRERYLALREDPRFKLIIIFKNHGSK
                     AGTSLEHPHSQLVAMPIVPLTIRYRFEKAYTHFDDEGSCVYCDVLREGFKQPTRLVLE
                     TDRFAAFHPFASRAPFETWIIPKAHNASFGSISMDGAKEFAYVLKKTLGKLYRGLNNP
                     DYNYIIHTAPIKDEQEEYFHWHLQVLPRLVTPAGFELGTGMFINVALPEETAAYIRNV
                     PD"
BASE COUNT         7380 a         8933 c         8979 g         7198 t
ORIGIN      
        1 gtcatgccgg cgttatgggg agagatgacc acgttgggca gggaaagaat gggatcgtcg
       61 gcgggcaggg gttcccggtc gtacacatcc agagcggcgg cagcgagcct tcccgtccgg
      121 agggcctcga tcatggcccc cgtgtccacg atgctggccc gggctgtgtt caccaggatg
      181 caggagggtt ttacctttgc cagggcctgc cgggaaatga gcccccgggt cttttccgaa
      241 gccttgatgg tcacggcgat cacatccgat tgggacagga gttggtcgaa ttccacaaac
      301 tgaattcctg cctcctcggc tctctggggg ctggggttga aagtccaggc ccaaaccttc
      361 atcccgattc ccctcgccag gcctgcaaac tggcgcccga tatttccaaa gccgataatc
      421 cccattctct ttccgtgaag ctgggtcatg gggaagcggg tccatcttcc ggcacgaagc
      481 tcctgctcat gcccgggaat tttccgggct gcggcgagca ttaaggccag ggtgtgctcc
      541 gctacggaaa cggcggagaa ccccggggtg ttggtcaccc tgatacccag ccgggaagcg
      601 gcgcccaggt ccacattgtc cgtaccgact cccagaacgg cgatcattct taaatccggg
      661 caggccttca ggaggggttc atcgaacttg gagtaagccc ggatgttgac cgccactata
      721 gctccctgaa gcctttgaat cagctcctcc ggagtggagg cctttttcgt atacaccgtc
      781 accttggcgt gcagctcgag tcttttcaca gccgcggttc ccgcaaggat ggaaggaaaa
      841 tcatcgggga caactaccga ttccattttt tcaccccact tcaaatgcca aagtttatca
      901 tacggaccta gtgcaggatc ccctttttca gcttcttcag ctcctcctca aagcctggtt
      961 tgaacgttga cactgcctgc tcccattccg ggcttccctc gactttggga tggaccgccg
     1021 aaccgctgat gccgaggcgt tccagcccgg ccagcaattc ggttttggtt ttcagagatt
     1081 catccttttt ccgaagttgg tacctctgaa gcaggtcttt aatcttcttt accgtccagg
     1141 cacttttcgg gataagggat tccagcccct gaaggatcag gtcgttgtcc cggtccagat
     1201 tcattgcctc gatcagatgc tgaaggaaca gctgctccag atgtttccgc tgctggggat
     1261 cgaatttggc gagttctttc tccacatccc ggagatgaaa atccacctcc aggaatcggt
     1321 tcaccaggcc gatcgccttg gactggagct cttcttcctt cattttctcc atctcttcat
     1381 gggaaagacg aagccccttg gtcctttcca gggccagctc aaccgtgctt ttgatttcag
     1441 ccatgattca acctccggat caataaaact gaacctttcc tgcggcagag aaaaacaaag
     1501 aaattttacc aaaaaagctc accctcttca acccgcctta ggccgccaga aaggcagtgg
     1561 ttttcatatt cagcattccc cactccgaat tccgattttc aaggcccttg actccctccc
     1621 atttaatgct tactgttttt atagattcgg ggtcgggatt gggaggggac catgccgcct
     1681 aaggattttg accagtttga agaccaagtg taccgcttga tgagcgatat gtttaaggac
     1741 gccaaacctc tgggctatca gccggacagg tgtttttccc cgccgatgga tatctatgaa
     1801 acggaggaga acctggtggt ggtcatggag atcgccggga tgaagcccga aaacattcag
     1861 gtgaccttcg aaaaggatat tctttcgatc cggggcttcc ggaacgagcc atcttccccg
     1921 cccaagacac ggctccatca ggtggagatc gattatggaa gattccagcg cactctccgg
     1981 attcctttcc ccctgaaatc cgatgatttc aaagccattt accggctggg cttcctcgtg
     2041 gtcaccgtcc cgaaagcgaa aaggccggtc tccctcaacg tggaggtgaa cgttcgatga
     2101 aagatgagat cgagacgatc atcgggaatc tgtcggctca ggaggtagcg gagaagggcg
     2161 aagaagggaa ggggatggag attcccgaaa accttcccat ccttcccatt cgcgagtcgg
     2221 ttctgtttcc caaaatgctc cttccgctca tggtcagcca ggaacgccat gtcaagctca
     2281 tcgacgcagc cttgcttgcc aataagatga tcggaatcgc ggccatcaag aaaaaagggg
     2341 tgaccgaagt caagccggaa gacctgtacg aagtcgggac tgcggcttac atcctcaaga
     2401 tgctgaaaat gcccaacgac agcctccggc tgctgattca gggcatctcc cgcatccgcg
     2461 tcggtgcatt tacccagcag gagcccttcc ttcgggccca agtgacgact ctggccgagc
     2521 aaggggaaaa gaccaccgaa acgcaggcct tgatggcggg ggtgaaaggg atcttccaga
     2581 agtttgtgga gctggctccc aaccttcctg cggacctggc catcatggcc atgaacgtgg
     2641 aagagcccgg ggttctggct gacctgatcg cttccacttt gaacctttcc ctggaagaca
     2701 agcaggccat tctggagagc atggaggtca aggcgcggct ggagaaggtc aatcttctgc
     2761 tcaacaaaga gcttcaggtt cttgaactgg ggtccaagat ccagacccag gtccgggaag
     2821 ggatcgacaa gtcccaaagg gaatatttcc tgcgggagca gctcaaagcc attcagaagg
     2881 agctggggga aaaggacgag cggactgcgg aaatcgaaga actgcgcgaa cggatcgcca
     2941 aggccaagct tcctccggag gcactgaaag aagccgaacg ggagctggac cgcctggcca
     3001 agatgccgcc gtcggccccg gaatacaccg tcgcccgaac gtaccttgac tggctgattg
     3061 aacttccctg ggcggtggcc accgaggaca acctggagat tcaaccggcc cagaaggtcc
     3121 tggatgaaga tcactacgac ctggaaaagg tgaagaagcg catcctggaa tatctctcgg
     3181 tccgcaagct gaaggcggac atgaaaggtc cgatcctctg ctttgtgggg ccaccgggaa
     3241 cgggcaagac ttcggtggga aaatcgatcg ccaaggcgct ggggagaaaa ttcatccgca
     3301 tgtccctggg cggggtgcgc gatgaggcgg aggtccgcgg ccaccgccgc acctacgtgg
     3361 gggcccttcc cggaaggatc atccagggga tccgcaaggc cggatcgaat aatcccgtct
     3421 tcatgctgga cgaaatcgac aagctgggta tggatttccg cggagacccc tcctccgcgc
     3481 tgctggaagt cctggacccg gagcagaatt actccttctc cgaccactac ctggaggtcc
     3541 catttgacct cagcaaggtt atgttcatca ccacggcgaa catcctggac cccatccccc
     3601 ccgccctgcg tgaccgcatg gaggtcctgg acctccccgg atacaccgaa gaggagaagc
     3661 tccgaatcgc caaggacttc ctggtgccca agcagaccca ggcccacggg cttaccgatc
     3721 aaaccatcca gtttcaagaa gacgccctga gacggatcgc tctggagtac acccgggaag
     3781 ccggagtccg caacctggaa cgggagatcg ccaacatctg ccggggagta gcccggaagg
     3841 tggcagaagg tgcgcagggg ctcaccatcg tggacgcgga aagcgttcct acgtacctgg
     3901 gcccgatcaa attcttctcc gaggtcgccg aacggacgtc ggagcccgga gtcgctaccg
     3961 gcttggcctg gacacccacg gggggagaca ttctcttcgt ggaagccacc cgcatgaaag
     4021 gccgcaaagg cttcagcttg accggtcagt taggggaggt catgaaagaa tcggcccaag
     4081 cggccttgag ctatgtacgg gcgaaagcca aggccctgaa tattcccgat aactttttcg
     4141 actccagcga tattcatatc cacgttcctg cgggggccat tcccaaggac ggtccttcag
     4201 caggggtgac catcttcacc gccatcacct ccctgctcac cgcaagaccg gtgcgcagcg
     4261 atgtggccat gaccggcgaa attactctcc ggggactggt tcttcccgtg ggagggatca
     4321 aggagaaggt cctggcggcg aggcgggcgg ggattaccac cctgatcctt cccaagaaaa
     4381 acgagaagga cctggaggaa gttccggagc aggtgaagaa ggacctgaaa ttccatttcg
     4441 ttcaaagaat ggacgaagtg atccacattg ctttgaagcc gaaaatgaag cccgcccaaa
     4501 agcctaaatc gaagaaataa ggcctcgcca ttttcaatcc ccctcctcta ctggtcaaag
     4561 gaggagggga gggcaatcct cgttccgcac ccggcaagga aaaagaaagc cttctttctc
     4621 ttgggcgaga aagaaggctt taggctgggt gataaccgat ttttgttacg caaaaggagt
     4681 catcgcgaga aaggcgggct taggtgtgtg tggggtcaaa ttggctcttg gcgatgatcg
     4741 actccctttg cctgtcctca ttgttaggcg caccacctcc tttccgaatc aggagtcctc
     4801 gtctccaggc tcctgatact caaagacctt tccttgataa ttccgcaaga ccatccttcc
     4861 tttgtcctgg gaaatcaagt aggtcggtcc cacgggtatt ttgcccccgc cgccgggggc
     4921 gtcgatcaca aagctgggaa cagcgtaccc ggttgtgtgg ccgcgaagtt tctcgatgat
     4981 gttgattcct acggcgatgg gagtccgaaa atgctcggtg cccatggcca ggtcacattg
     5041 atagatgtag taggggcgaa cccgagtttt caggagctca tgcatcaacc gtttcatgat
     5101 ggagggcttg tcgttaatgc cccgcagaag aacggtctga cttcccaggg gaattccggc
     5161 atcggacagg agaccgcagg accgccgcac ctctttggtg atctccttcg gatggctgaa
     5221 gtggatgctc atccacaggg gatggtattt cctcagcatg ttggtcagag ccggggtaat
     5281 gcgctgggga agggtcaccg gcacccgggt tccgatgcgc acgatttcca cgtgggggat
     5341 ttttctcaac ttggtcagga ttctttccaa gtggtcgtct tccagaagca ggggatcgcc
     5401 cccggaaatc aaaacatccc ggaccttttt ggtggcctgg atgtagttga tggcttcatc
     5461 catttcttct tcggtaatgc acttttcact cgaacccacc attcttcttc gagtgcagta
     5521 gcggcagtat accgcgcact tgtcggtgac caggaggaga acgcggtccg ggtatcggtg
     5581 aaccaggcca tggaccggtg aattttcatc ctcggcgcag gggtccaaaa gatcgtgagg
     5641 agaaaagtga atttcctgaa gagtggggag ggcctgccgg cggatgggac agttgggatc
     5701 ggtccggctc atcaatgtgg cgaagtaggg ggtgatggcg gtggccatcc ggcctttgga
     5761 ggcgtccatg gcagccactt cttcatcatt caggttcaag agaagtctga gctgttcggc
     5821 cttggtgatc cggtttctta actgccagcg ccagtcttcc cactcttcat cggtggcatc
     5881 cttccatgga ttcggctgat attcttcctc cacggggatt ttggcctcag ccagccggat
     5941 agatcggatg ggctcccgag ggagcacggt actgccagaa acgctcatgg tcacctctca
     6001 ggttttggac tccgtgggtc cgggtttttg agcttgcgag tcgagcctga gcttcggact
     6061 ctgcagttcc tccaaagctt tttcgtaagc aatcttatgt ttgaagccgc tttgaaggta
     6121 cttccagatc aattcgagtt ttctgaattc ttcttcgctg tattctctgg actccagttc
     6181 ccccataggg atccgtttcg gcttgatata ccccttctgc tccaagtaat acaatttgtg
     6241 ccttggaata tttagctttt ttaaaatgtc cgaggtcctc atggcgaaac tccaagagga
     6301 gcgtatagtt tcaatcatca atgaaaataa atccaaatct atatatagat ttaaatctat
     6361 ttttatacag tgtaaaagaa tggaaaaaag ctgtcaagaa aaaaatgata tcttgtaaaa
     6421 aaaatttttc cccttggccg acgcattaag aaaggaattt ctgtatccgc gggaccatcc
     6481 ctcatcaggc ggtaatattt taataattgg gcttaggatt ctggaattca gacggattga
     6541 ggatggctgg attgagcgcg aagatcggaa ctcattccct aatcttgggc tccggggtag
     6601 attccgtaac gttgcagggc attccacatg gcgatgatgt tttccggcgg ggtctcgggc
     6661 tggatagcct gggtagcggc gaagacgaat cctcctcccg gggcaaggtc ctcgatgcgc
     6721 cgctttacct cgtcttccac ttgctgcggg gttgaaaaag gaagggtgtg ctgaatatcc
     6781 actccgccgc cccagaaggt gagggcgtcg ccgaagtcct tttttaattt tcgcgtatcc
     6841 cccatatctc ttgcccctag ttggattgga tttaagattt ccactcccat ctcaatgata
     6901 tcggggatga gcgggtaaat ggcaccgtcc gagtggagga gaattttctt cccgtaccgc
     6961 tttttgatga aagagaagag ctccacgtag cggggtttga ttagcttacg gaacatttcg
     7021 aacgaaatcc acaggctgtg ctgatgcccg aaatcatcca tctcgcatac gatatcgatc
     7081 catttcccca ttcggtccaa gacattttgg taaaagctaa gcttcaggtg gaggagcttg
     7141 tccaagagcc attccgttcg taggggatcg agaacgagat cggtaaagaa acgttcgtgc
     7201 ccctcgagct gggctccgaa ttggagcagg cccgaggaaa aagagctccc aaggacaaaa
     7261 gccggatcga cctgcctgcg gacagccaat acttccttct ccacccccgc aagacggttg
     7321 gggtccagcg ggtccggcca gggaaagcgg gggagatcgg aggtttccac ttctgacaaa
     7381 ggttgatgga atatgaagaa atgcatcccc cggtccttgg ctagcttccg tccgatcccc
     7441 cattcatcat agaaaaagga atatcctccc tcttcctgca tcttcaatga ccaagccccg
     7501 tgagggcgga cccggaaaga gcgggtgtcc actcggaacc tctgcagaaa atcttcgctt
     7561 aatttcgccc gctggctggc catatccccg acctccgccc tttctcctga atggatgtac
     7621 ttcagcaggt tacggtaagc ctggatggac attccgctgg ggcccaagga ccccaaatca
     7681 taggggaccc ggtcgggttc ccgatgggaa agggcggtca ggattcgttc ccgggaagtc
     7741 ataaatgttc agtctggggt ggattttggg gggtcgttgc cagcagccga ctaggggcga
     7801 ttcaccaatc gcccctgcgg cttctcgatg ggtgggaaaa ggctaaagcc ggtccttaaa
     7861 caccgccccc gttcccgccg aagtcaccaa ctgggcatac cgggcggcat agccggaggt
     7921 gatcttgggc ttgcgcggtt tccaggattt ctttctcctt ttcagctctt cttcattgac
     7981 cagcagggtc agtttctttt taggaatgtc gatccggatt cggtcgccgt tcctgaccag
     8041 ggcgatcggc cctccctccg cagcttccgg agagacgtgg ccgatagccg ctccccggga
     8101 acccccgctg aagcggccat cggtaatcag ggcgacgtct ttatccagtc ccattcccgt
     8161 aatagcggaa gtgggagaca gcatctcctg catccccggc ccacctttcg gtccttcgta
     8221 ccgcacgatc acgatatggc ccggcttgac ctttccccgc agaatggcgc ccatggcttc
     8281 cggttccgat tcaaaaacga tcgcccttcc ctcgttgacc aacatctccg cggccaccgc
     8341 ggactgcttg accaccgccc cgccgggagc caggtttccc cgcaggatgg ccagccctcc
     8401 ttctttatgg tagggattgg tgagggggcg aatcaccttg tagtccttga ctttgacccc
     8461 tttgaggttc tctcccaggg tcttgccggt ggcggtcatg accttccgat cgatgactcc
     8521 caacttggac aattccgcca tgacggcttg aacccccccc gcggcatcca gatcgtgcag
     8581 atgatggggg ccggcggggc ttaagaagca gaggtgagga gtccggtcgc tgactttgtt
     8641 gaagaggtcg agattcagat cgatcttggc ttcatgggcg atcgctggaa ggtgcaagac
     8701 ggtgttggtg gaacatccca gagccatatc cgcggccatg gcatttttga acgccgccgc
     8761 ggtggcgatg tcccggggct tgatgttccg cgtccaaagt tccatgacct tcatccccgc
     8821 gattttggcc aggcggatcc gggccgcgtt aaccgccggg acggttccgt ttcccggcag
     8881 ggctaggccc accgcttccg acatgcagtt catggaattg gcggtgaaca tccccgcgca
     8941 ggagccgcac ccgggacagg cgcaatcttc cagctccttc agccgggccc ggttccattc
     9001 cccgcgggcg acccgtccga ccccttcgaa aacgctgatc aggtccacct ccttatcagc
     9061 gacctttccg gtcatcatag ggcctccgct gatgacgatg gagggaatat tgacccgcaa
     9121 tgcggccata agcatcccgg gaacaatctt gtcgcagttg ggaaccatga ccatggcgtc
     9181 gaagggatgg gccatggcca tgatctccac ggagtcagcg atgagctccc ggcttgcgag
     9241 ggagtatttc atcccctcgt gattcatggc gattccgtca cacaccccga tggtggaaaa
     9301 ctccagaggg gtgccaccgg ccattcgaat tccggccttc accgcctcta cgatccggtc
     9361 gaggtggata tgtccgggga tcaattcatt ggccgagttg gcgatcccca ccatgggccg
     9421 ccggatctcc tcttccgtaa gccccatagc cttaaagagc gaccggtgag gcgccttctc
     9481 caatcctttc ttcatcaaat cactgcgcat gcctcaaacc tccactttat tgaattctgt
     9541 catttattcg cagtattttg aaccctttgc atttgcccag tgctcagatc ttcctttgga
     9601 tcataccttc gctcagatta ccttatcaag tgaaaaatag gaaaaggaaa acaggttcgc
     9661 acagaaaaca ggttcgtact tgacagcgag attccttgcc cctagaatca tttttaataa
     9721 atgagggata tgatggggcc ttcgattcta gcccgatggg ttagggccta agaggaaata
     9781 atctggaagt ttctttagcg gaacctgggc tcatagaaat acttgagaaa ttttggtaat
     9841 ctgaattgaa aaagtaacgg catgaagagg gaatacgtca aaatcccgaa aaggatttct
     9901 tccggaagag atttatggtt tctggcaaaa cccaattggc aaccttaacc catcgcggaa
     9961 aatatcgagg cgcccgaccg caccctgaat tcaccctccg tgaaaagcaa atcccattgt
    10021 ttgcctcttc ggccaacgga aatggacagg ccgctttccg tgatccggct ttcacggaga
    10081 ataaaaacct accggtgcat cggtgggtcc cctggattgc aggttattcg gcctcctttg
    10141 tggatgatgc gatttcctcc tatctcccgc gtggaagaac gggaatggta ttagacccgt
    10201 tttgcggggt cggaacgaca ttggtgcagt cggttattcg aggccatgac gcaatcgggt
    10261 ttgagatcaa tccttatgcc gcactcgcga cgcttgccaa gctcagagcg cttacgatcg
    10321 atgaatcaga ttttaccaaa gcgatcactt ctttacgaaa aaattcaatc gggtggaaaa
    10381 caaattcccc cccggaggat atcaagaccc ctccgttcaa gtcgcgcctt ccttttttca
    10441 gcccgaaggt ggagaggcag atacttcatg ctcttgcttt tataaaggcc attgaatctc
    10501 aacctatcgg tgatttattt cgcgttgcct tcggggcggt tatggtgagt gtttccaact
    10561 attcttatga gccgagtctt ggctcccgtc cggcagccgg caaacccttg gtagatgatg
    10621 ccgaggtagg ccagattttg ttgaacaaac ttagtcaaat gaggacggat atcatctggg
    10681 tacaccaaga aattaaggga aaaagcttgg gaaccggaag agtcatcaac gctgattatt
    10741 ttaaggtgaa cggtcagctg ggaaatggaa gcatagatct catgataacc tccccccctt
    10801 atatgaacaa ctaccattat gttcggaata cacgcccaca attgtattgg ctcaatttta
    10861 tttcctcccc aaccgaacag aagcacttgg aaacaaataa tttcgggcaa tactggcaga
    10921 ccgtaaggga tgcgagcccg gtttctctta cttttgagca taaggagctg gaaaaaatcc
    10981 tgaaaaagct tcgtgaaacg agaatagata aaggtgctta cggagggcca ggttgggcta
    11041 attatgtggc ttcgtatttt aatgattcct accgctttat gtcgagcctt aaatatctac
    11101 tggcccgggg gggcgtaggg gtgattgtca ttggaaattc catcattcag ggcatagaca
    11161 tcaagaccga gaaaatcctg tcagaaatag gagaaatcgt agggttgatt cccgaagggg
    11221 tgcattgtat ccgtgagaaa cgcgttggag caagcattac tcagtcatcc gtccgctacg
    11281 gggagctgaa cagagcggtt ttgtcagaat ccgcagttat tattcgtaag cgataagatt
    11341 atggggaacg aaccctataa ccctttggat aaactccgtt tgggggagag tgtaaccaag
    11401 gctttgctgg ctcgcccggt cgtacctttg cccatccccg agccatttgc cggagcgggt
    11461 atctatgcta tctattacgc tgggaagttt tctccctacc ataagatcgc ggaaaagaac
    11521 ataaataacc aatgggctgc gccaatttat gttggtaaag cagtcccgcc gggcgcaaga
    11581 aaaggaggat acggattgga ccaatcgccg ggcgatgttt tgtatcggcg attgcgtgag
    11641 cacgccgaat ccattcagca ggctgaaaat cttctgattt ccgattttag atgtcgtcat
    11701 ttgatcgttg atgatatttg gatccccttg ggcgagtcgt tgttgatttc gatgtttttc
    11761 ccgctttgga accaggtcct tgatggattc ggcaaccatg accctggtgc gggtcgctat
    11821 aaccagcaac gatctccatg ggatgtaatc cacccggggc ggccttgggc ggcaaagctc
    11881 agaccgaatt cccgatcgga agaagagatc ttaagaagca tctctgagtt tcgaccatag
    11941 atttcttcat ttatatggtc atgggtttga tttttggcag gaaagaatcg cgaccttgct
    12001 ttgtcctcgc tgaacgaagg aatctgctga ttcatttttt tctccaatct taagaccttt
    12061 tcccgatttt taataaaacc aaagcggatt agaagatcag gtatgatgat catcttgcat
    12121 gccggtatat ttgaagggaa gtttttattg tggggagaat cgtcctccat ggaagctcct
    12181 ctggaaaaaa ggagaggccg acgaaagaat tcaaagtctt tcttaggacc ggggaaacca
    12241 tatccatatg acgccggggg accgaaatta tccgccgggc tgaaagaagt gggatttcat
    12301 ttttcggcca gcaagaaatc attcggaacc ttcaccgcct ggttgcccgc ctcggacgcc
    12361 cggccggtcg cctccagctc tttggttgcc gacccgccgg gtccgcagga tgaaacgatc
    12421 ctccagccct ggacgataac cgccctccgc ctctccccag cgcaaaccgt ggagctcctc
    12481 tgtgcctcca tgggaaagca gattctttcc cccggaatcg tcgttgggaa agatgtcttc
    12541 ttctgggcgc aagccctgcg cttcgccgca tccctggtag cccgacagca gttcctgccc
    12601 ggggttgctg aggaggatgg aggggtctcg gcgaaatgga aaccggtcat ccacggcccg
    12661 gacggcgaac gctttgccgt cttggctggg gccatgccgg ccgtttcccg ggcggtcatg
    12721 gggacgggaa aatcactgca aacgacttcg gatgcccctg ccgccccgtc tcctgtttcc
    12781 ctgctctcct tttttatcga gaaggttgtc gatcacctgg tccggtccgc gggtctgtgg
    12841 ctgaacacgg gcgggctcag gggggattcg aaggactttg aaagctccca cgaccagtgg
    12901 ctgcactccc tacgagcggg cgacggaacc atggccggca gccctacgga atgggcggag
    12961 ctcaccgggc agatacaaga gtggcagcgc cctctggcaa tctcggcggc cagtcctttc
    13021 cggctttgtt tccgtcttga agaacccaac ggcaacgaag accaaaagga aaaggccgga
    13081 ttgaggatgg cctcctggta tgttcgctat ctgcttcagg ccgcacagga ccccagcctg
    13141 ctgattcccg cggaggaagc ctggaaggcg aaagatagag cggggaaaaa aggaaggtcc
    13201 tcttctccgc tgcttcagga tggtttcgag ccgcgggagt atcttctcct gtccctcggc
    13261 caggcctcgg ggatatggcc gcgaatcgag gccagcctgg caaagccggc accggcaggt
    13321 tatgaactgg atgccgcagg agcccacgac tttctcactc aaaaagccct ggctttacga
    13381 caggccggct acggggtcat gctgcccgcc tggtggacgg gcctgggaac gaaggaccgc
    13441 ctcactgccc gggccagcgt ccaggcgccc cggatgcagg gaacaggggg gctctctctc
    13501 gacgaaatcg tgaaattcga ttgggaagtg gccctggggg gcgaagggat ttctttcgaa
    13561 gagcttcaag ccctggcccg gctcaaagtc cccctggtca agctccgcgg ccagtgggtg
    13621 caggtgaacg ccgaggagat cgaggcggcc ctcgccctct ggaagaagaa gggccaggcg
    13681 acggcccggg agattgtcca aatggccctg gggggaagca ggatctccgg ccagatcccg
    13741 attgacggct tgcgggcgac gggctggatc tccgacctgc tggcggagct ggagggaaag
    13801 actccttttg aggaattacc cgctccggct gaattccagg ggaccctgcg gccctaccag
    13861 gtccggggtt attcgtggct cgccttcctg cggaaatggg ggctgggcgc ctgcctagcc
    13921 gatgatatgg gcctgggcaa gaccattcag gccctggccc tcattcagag ggattggcat
    13981 gcattcgtca agcgaccggt gcttctggtc tgccccacca cggtggtcaa caactggcgg
    14041 aaagagacgg cccgcttcac ccccgatctt ccggtcatgg tccatcacgg catcctccgg
    14101 atgaggggag aggacttcag gcaggaagcc ttgaagaacg ccgtcgtcat ttcaagttat
    14161 gccctgctgc accgcgattt tgaaaccctg aaggaaattc cctggtccgg gatcatcctg
    14221 gatgaagccc agaacatcaa aaacccggaa accaagcagg cccgggccgc ccggggtttc
    14281 caggcggatt accgcctcgc cctgaccggc acgccggtgg aaaacaacgt gggcgacctc
    14341 tggtcgatca tggaattcct gaaccccggt tttttgggca cccaggccgc cttcaagaga
    14401 gggttcttca ttcccatcca ggcggcccgc gacccgcagg ccatggagcg attgaagcgg
    14461 ctgaccggtc ctttcatctt gcgccggctg aagacggaca aaacggtcat cgccgacctg
    14521 cccgaaaaaa tggagatgaa ggtcttctgc tccctgacca aagaacaggc ctccctttac
    14581 gaagcggtgg tgaaggagac gatgaagacg ctcgatgcga ccgagggaat ccaacggaag
    14641 ggagccgttc tggccaccct ttccaaactc aaacaggtct gcaaccaccc ggcccaattc
    14701 ctcggagaca actcgcccct ccctggccgg tcgggaaaac tggcccgcct ggccgagatg
    14761 gtagaagaaa tgatggccgt gggggaccgg gccctgatct tttcccagtt cgcggagatg
    14821 ggggagatcc tgaggaggta tttgcaggaa accttcggcc aggaagtgct ctttctccac
    14881 ggcgccgttc ccaagggaca gcgggaccgg atggtggaac gatttcaagg agcgcaagac
    14941 gggccttcca tctttatcct ttccttgaag gccgggggaa ccggattgaa tctgacccgg
    15001 gccaaccatg tattccattt cgaccggtgg tggaacccgg cggtggagaa tcaggccacc
    15061 gaccgggcct ttcgcatcgg ccagacgaaa aacgtccagg tgcacaagtt tctctgcgcg
    15121 ggaacgctgg aggagaagat cgacgagatg atcgagcgga aaaaggggat cgccgagaag
    15181 gtcatcggaa cgggcgaagg ctggctcacc gagctttcca cggcggagct gagggacctt
    15241 ttttcgttga ggaaagaggc catgggaacg ggggaataaa atgcgccgat gggatcatta
    15301 caggtttttt gaaccctccg ttccccgccg ggcaaagggg ggaatccggg cccagtccaa
    15361 gcggggcacc ttcggggaaa gctggtgggc gaagcgctgg atttccgtcc tggagagctt
    15421 cgacatcgga gcgaggatgg gccggggccg gaactacgca cgaagcgggc aggtcctctc
    15481 cattgatatc gaaaaaggag ccgtgaaggc caaggtccag gggtccaggc ccacccccta
    15541 ccgagttgcc gtcgagatca aatcgatatc ctacgcgaac tgggagaaac tggtcaaagc
    15601 cttgtctcat caggccattt tcgtggccaa gctcctggct ggagagatgc cgcaggatat
    15661 tgagaaggtc ttccaggcgg cgggcttgtc gttatttccc gacaaatcca aagacctaaa
    15721 gacggaatgc tcctgcccag actggtcgaa cccgtgcaag cacatcgccg cggtctatta
    15781 tctgctgggc gaagagttcg accgggaccc cttcctgatc ttcaaactcc gcggattgca
    15841 gcgggaagaa ctgattcaaa ggctggggaa cagggagaaa agatcggata ggaaaaaaag
    15901 aaaatccaaa ccgcctcccg cgcacgatga agaaaaaaag attcctatct cggaatcgct
    15961 gcctgcggat gggtccctct tctggaccgg cggcgccctg cccgaagatt tctttggaga
    16021 ggttcaggtc cccccggttt cggcggctct tctgaagagg ttggggaaat ttcccttctg
    16081 gcggggaaag gagcgtttct tcgatgccct ggaaccggtc tatactcggg cttcgactcg
    16141 cggcctggcc gttttcctgg gagagaaaaa cggccctgag gttccctcga agtaggtttt
    16201 ctcctatctc gtcaagctgt aaatgcctct ttcccctttc tctccccgcg gctctcccgc
    16261 ttctcctggt cgggattgtt cctctattat gagaagatac ggggcataaa ctcccggggg
    16321 gtgggtctcc aggaatgctt gacggttgag agcgggggga gtaaagaagc ctaagggcga
    16381 ggaaggagat cccgggccag gagagcggcg ccgagggcac cgatgatttg gggttcagga
    16441 gggacaaaca ttcttactcc cagccgatcc tccagcgccc ggacgacccc ttcattcttg
    16501 gccacgccac cggtaagagt cacctcttcc tcgattccta accggttcac catccccagg
    16561 attcggtccg caatcgagtc atggagaccc cggatgatca cctctttggg ttgattgtcg
    16621 gcaatcaggc tgaccacctc ggactcggca aagaccgtac acatgctgga aatcttaatc
    16681 gtccgcgggg cgatgaggga gagcctcccc aagtcctcta ctttcacctc cagggcatgg
    16741 gccatgacct ccaggaaccg cccggtcccg gcggcgcact tgtcattcat ctgaaaatca
    16801 acgtttcttc cctgggcgtc cagccggatg actttgctgt cctgtccacc gatatcaatc
    16861 accgtccgcg tttggggatg aacgaagaaa gccccccggg cgtggcaggt gatctccgtg
    16921 atcctctttg tcgccccggg gaatgaagcc cttccatagc ccgtggtgac gatgacagtt
    16981 aggtctccct gattaagcga ggtcttttcc agagcggctg acaggcagcg ctccgccgca
    17041 ttccggctgt tggctccagt gggcataatg acggaggtga ggatattccg gtctcggaga
    17101 atcacggttt cggtggtgat ggacccgata tccattccgg caacgatcat agacgtccgt
    17161 cccttgtgag aaatttccaa gtggagtata tttagcggca gcggaaaaaa caagaataaa
    17221 aaacacagag ctcacaaggg atacctcata gccccagagc cgatcacagg ccccgaaaac
    17281 tctgagtcaa gacccataat tcatgagcct aacgggagat tccatggact ttcttctttc
    17341 cgaagagcag aagatggtgc aaaagttggc cagggacttt gcggagaagg aactgaagcc
    17401 ccgggctgta gagaccgaag ccgcccgcca gttgcctctt tcgatcctga aggccatggc
    17461 cgatctgggg atgatgggaa tgaatgttcc cgcggaatac gggggggcgc agaccggcgt
    17521 ggtaagctac agcctagcca tgaccgagat tggaaaaggc tgcgcctcca cggccgtgac
    17581 catggccgtg accaatatgg tggcggaagt gatccacgcc ttcgggagag aagagcatcg
    17641 gcgaaatcat attcctaaaa tttgcggcgg cgagtatcct tccggggcct ttgccctgac
    17701 cgaatccacg gcgggctcgg acgcatcctc catgaagtcc accgccgaac gatcggggga
    17761 ttattacctc ctcaatggtt ccaagacttt tatcaccagc ggtgactttg cggggatttt
    17821 cgtggtttgg gccaagaccg ctaaaaccac cggggccaaa ggaatcagcg ctttcctggt
    17881 ggagcaggga acgcccgggt tcaaggtcgg gcgcaacgag gagaagatgg gtcagagggg
    17941 ttcgcccacc aacgagatct ccatcgaaga ctgccgttta ccccgggatt ctatcctggg
    18001 ccgggaagga gacggattca gaattgccat gatggctttg gacgggggga ggatcgggat
    18061 cggttccttg gccgtgggga ttggccttgc ggcgatggaa tatgccaccg aatacgccaa
    18121 gaaccgcatc cagtttggga aacccatctc ttccttccaa gctctccaat ggatgattgc
    18181 cgacagcttt accgagctgg aggcagcacg gctcctggtg ctgcgggcgg cgcacaggaa
    18241 agagagaggt ctgcacttca cccgggaggc ttccatgggc aaactcttgg ccacggagac
    18301 ggccaaccga gtctgttaca aagcgttgca gatgctgggg ggatacggat atatgaagga
    18361 atatcccctg gaacggtata cccgcgactg ccgggtaact acgatctacg aaggaacctc
    18421 ggaaatccaa cggctggtaa tcgccaggga gattctgggg ggcgactacg tatgagaaag
    18481 tttcgagttg ccggtttcga gtgaaaacat actaaaaaga ggttttttct ttaaacccga
    18541 aactcttttt agatggcttc attcaccggt tgcaattcct tgtagtctcc tttggccttg
    18601 gccttctcca ggagattctc tatccagctt cccaggatcg tctcctgctt ctgcatcaga
    18661 agacctcgcc ggtaattctc tttttgggaa tcgaattggg cctgatcgat ctccttaact
    18721 tcttttagat agagaatcga gtatttccca tcttggaaga ttgggctttc ggggtaaggg
    18781 ttcctcaccg agagagcagt cagggctttg agcatatctt ccgaggggga gactttgggg
    18841 ggggctgaag ctcgagggaa aaatcccgtc tcctcaatct tcaacccctc ctgggaggct
    18901 agggccttga aggcgcttcc ctttttcaac ttttccagca gctctttggc tttggccaag
    18961 gccatgtcct tttgcttttc cttccgcagg gcctcggtga cttttccttc gacctctttc
    19021 aggtccggag tgcgggattc ctgcttctcc acggcctgca tcaccgcaaa agtctccccc
    19081 acccgcagcg gagggctgac atctcctttc cccatggaca aggccgaagt tttcagcttg
    19141 ggatcaaggt caattttatc ctccgcagag aataggagag tttcctttac ggtgagtttc
    19201 ttctcctctg caaacttgtc cagggtttta tccttggatg cccgggcaaa ggcctgatcg
    19261 gccatgtcgt aagccaattc ccgggcctgg cgattcttga ggaggacctc gatctggtcc
    19321 ttgacttttt ccatgggctg ggtgctggcc tcctgaattt cgtccgcctt caggatgtga
    19381 aagccatagg gggtctgaat gacgctgctg atctcccccg ctttcatgga gaaagcggcc
    19441 tgctcaaact cgggcaccac cattcccttg gagataaagc cgatttcccc gccccgatcc
    19501 ttggttcccg ggtcctcgga gtacttcttg gccagttgag cgaaatcctt cccttgcgcc
    19561 gcttcttccc gaacggactc cgccttttgc cgggctttgg ccgacccctc tttgtcctga
    19621 gcctccgctt tgattaaaat gtgacgcacc ttcacccgct tgggccggcc gaacttctcc
    19681 ttgttgttct ggtagtagtc ttcgatttcc ttgaaggaga cggggatttg cttaaaaaag
    19741 tccttgggct cgaagaggag gtagcggatt tttactttgg tcgggacctt aaaatcctcc
    19801 ttatgcttgg agaaaaattc cttcaactcc tcgggggtga tggaaacctc cttgaagtcc
    19861 gccggattca gggaaatcat ttctaggttg attctttcga aactctgccg gtaggcctcc
    19921 tggacttccc gttcggaaac ccggatggag gagagaatca acccctggag cttgttgatg
    19981 agcagcaggt ctcgttggtt ggcctcgaat tccttggggt tcagccgggc acgctggaga
    20041 gcgcgagagt aggtggcttg gtcgaaaatc ccgtcttttt ggaatgcggg aatgctgtgg
    20101 atggaagctt ccatctcctc ttccgcgacc ttcaacccca gacgctgcgc ttcctgcagg
    20161 aggagaatcc ggttcaccag gtccttcacc gcccgctctt tcaggttgag ttttttggcc
    20221 atatcctcat tgaaacgctc tccatagatc gactgatagc ggtcccgcat gttttggtag
    20281 taattcctgg cttcggtgac cgtaataatg tatggtccga ttttaatcaa ttcccgttga
    20341 cgcccttccc ggggggccca gatgccccaa aagatgaaga cgaccacgac cgcccccaaa
    20401 gcggccttga tcatccagga ttgagcatgt ttcctcatga aatccagcat aaagcgcgat
    20461 cccctttccc caaggatatc aaagatttta tttgaatcag tccaaattga caagaaattt
    20521 ttccacgacc cacctgcccg tccgcaggag atgctttgaa aaatgcgggc caaggaccgg
    20581 gcagaaattt cttgcacccc agcagtattt ttggtatcat aatttcctta aatattctga
    20641 ggctttgctc cccgtcatcg gggaagggac ccttttcttc gggggaaata gaagaaagga
    20701 gcgggagaat gatttttgat tttgtcctgg gccttttctc gaacgacctg gcaatcgatc
    20761 tgggaacggc taccactctg gtttacgtga aaggaaaagg aatcgtcctt tgtgagcctt
    20821 cggtggtggc ggtgcgcaag gattcgaagg ggggacgcaa gattctggcg gtgggaaaag
    20881 aggccaagaa gatgctgggc cggaccccgg ggaacatcgt ggccatccgg ccgatgaaag
    20941 atggagtcat cgccgatttc gaaatcaccg aatccatgct ccgttacttc atcgcccagg
    21001 tacacaaggg ccggacgctg gtgcgtccga ggatcatcgt ctccgtccca tcgggaatta
    21061 cggaagtgga aaagagggcg gtgcgcgaat ccgcccagtc cgccggtgcc cgcgaggtct
    21121 acctgatcga agaacccatg gcagcggcca ttggagccag tcttccggtc aatgagccct
    21181 cgggaaacat gatcgtggac atcggcggcg ggacttgcga ggtggcggtc atctccctgg
    21241 ctggaatcgt gtatagccag tccgtccggg tcgggggaga caagatggat gaggccatca
    21301 ccaattttgt aaagcgcaag tacaatctgc tcatcggcga gcgcacggcg gagctgatca
    21361 aaatccagat cggaactgct taccccgatg aggatgtccg caccatggaa attaaaggcc
    21421 gggacctggt cgccgggaat cccaaagtcc tccagatcaa ctcggaagaa gtgcgggaag
    21481 cccttaccga gccgatcaac gccatcctag aggcagtcaa aaccactctc gaaagaaccc
    21541 cgccggagct ggccgcggac atcgtggata agggcatcgt tttggccggt ggagggtctc
    21601 tcctccggaa cctggatgtc ttgatccggg aggaaaccgg cctcccggtg acgatcacgg
    21661 aggatcccct ctccgccgta gtcctgggat cggggaaagc cctggatgaa ttgaacctcc
    21721 ttaaagaagt gacgatcaag acctgaaacc gtgcagtcgc atcgcgcctt gcgatagggg
    21781 caaaaagtaa agggcgaaaa tggctcgtag gctttcctct gagcgatcgg cccaatctgt
    21841 ttttttcttg ggcaatcccc ttttccgtga gtcagcccga tgctgaatct actcaaacgc
    21901 tttcgcctct tattcaccgc cctactcttt ctctttttgg ccctggtcct tatgaccatt
    21961 tacgccaaag aagggaggga aacctccctc atccagaagg ttttgctgca aatttccttc
    22021 cccttccagc acggggctca aaagaccttt ctttggttca aagaggtggg ggaagattat
    22081 ctttttcttt cccgggtccg acaggaaaac aaagacctca aacgggtcct cagcaccctc
    22141 caggaagaga acaatcgtct ccgggaagcc ctccagaccg acgagcgtct caagaagctt
    22201 tcggtctggc agtctcagta cccccacaag agccgggtgg cgcaaattta cgcccgaggg
    22261 ccctccagct ggttcaaaac ccttctgatc aataaagggg agaacgatgg agtggccaaa
    22321 gatatggccg tggccacctc cgaaggggtg gtcggccggg tcattgaagt gtctccgggt
    22381 accgccaagg tgctcctggt caccgatgcc aacagcgccg tcgatgtgat cgtccagcgc
    22441 tcgagggtgc agggaatcat ggaaggaacg atcgacgagg tttgcattct taaatacatc
    22501 cagaagaatg aagaagttca ggtaggcgat gtagtggtta cgtccggcca aggaggaatc
    22561 ttccccaagg gcttggtgat ggggaaagtg agtgaggtgg accggaagcg ccacggcatt
    22621 tttcagtata tcgaagtgac cccatcggtg gacctggcca aattggaaga agtcctagtt
    22681 ctggagaaat gatcgagacc ctgaagattc gaaggatcca accccaggat cgcccgagtc
    22741 tgaaagccct cctgcacgct caatcccact tccgtcccga agaaatccgg gtggccctgg
    22801 aactgatcga ccttgccctg gcccaacccg gcgaggaaga ttacgtcatt ttatgtgccg
    22861 aggaaaggga gggggagatt cggggataca tttgctatgg gaaagccccg cttaccgaag
    22921 cggtctatga catctattgg attgtcgtcc atcccgccta ctggagccag ggaacagggt
    22981 cttctctcct ccgccaggca gagggcgagc tagctcaacg ccaggcccgc ctgctgctca
    23041 tcgaaacttc gtccacgccc ccttatagga ttccgcgcgc cttttacggg aagcatgggt
    23101 accgcgaaca ggcccgggtc cgggactatt accgtccgga cgatcacaag atcatataca
    23161 gtaaaaacct aaagggaaga aatgaaactt gaagccgggg gtttgccact tgcgacctgt
    23221 gccctgagac ctgaatcttg agaaggggag agaatcagcg tataattcag ataagtcggt
    23281 tcaagaagag ctctggggtg cctcaccatg accgggatcg ctaaggtctg ggaaaagata
    23341 aaagaaattc atcgaagaag ggctcctttt ttccgaagac ccctgggact gcagacgcgg
    23401 attacccttc tatttgccgt tgtagtggtc agcgtcattg tccttttctc ttacctggaa
    23461 ttccgtttgg ccgaacattc ccaaagggag ctttggcggg agaggaccat ctacgtgacc
    23521 cgggaggtag acgccaagat ctacagctcc aaggacctgg aaaatgcagc ttttctggaa
    23581 gaagaaatcg ccaactggat gtatgcccgt ccttccatcc gatccatcga catcttcgtt
    23641 ttccaaaaga acacctataa gatcatggcc tccagctggg agggaaaaga cctgggaatc
    23701 agttatcaga gcctcgattc cttgaagagg gacatggttc tctccaccct gatcaagcgg
    23761 gatgatcatc cccactggga aatcctagcc ccgcttcatg cagggcgaaa gatcgtgggg
    23821 gggattcgca ttgtaacctc tctggaggag ttggagacct tcctggccaa taaacgcacc
    23881 cgcaccatcc tcttcaccct gatcagcgtc gccatcctta tcctcatcct ggccgtcttt
    23941 ttcagccggg ccatccaccg ccccatccaa cagctggtcc gggccatgtc cgaggccgag
    24001 tccgggaaac tgaacgtggc cgtcccggtt cggtcccagg atgagctggg tctcctgagt
    24061 gttcacttca atcggatgct ctcccgaatc agccagttca acgaggagct gacccagagg
    24121 atcgaggcag ccacccgtga gctggcccag aggaatgagg agcttcgcct ggccaatgag
    24181 tccctctttc aggcccaacg ccagttggtc caagcggaaa agctgtcggc cctggggcat
    24241 gtagcggcca ccatggccca tgaaatcggg actcccctca actccatctc cgggtatatc
    24301 cagctcatgc tcgccgagga gaccgggtcg gagttgatga ccaaacgtct ggtgatcatc
    24361 gaaacccagc ttgaccgact gacccaaaag atccgcaacc tgctccattc cacccgccag
    24421 cccgagcctc agctgcaggc agtggatgtc aatcatgtcc tggaaaccgt gatcaccctc
    24481 acccagcccg ggatgtccat tcgggccgtt caactggtgc gtcatttagg tccagacttg
    24541 ccccgaattt cgggcgaccc cagccttctc cagcaggtat ttctcaacct ggtgaccaac
    24601 gccctggatg ccatgcccca aggaggggtc ctcactctga tcaccgcccc gccggccgcc
    24661 tctggccgaa acgggtattt tgtagaggtg gtggtggagg atacgggaac cggcatgtcc
    24721 cccgaggtga agaagaaggc ccttgagcct ttttttacca ctaagtctcc ggggaaagga
    24781 gccgggctgg ggttgagcat ctgcgaagac atcattcgga gccatcacgg caaaatggag
    24841 atcgagagcc aggaaggaaa gggttcgacc ctgcgcattc aactcccagt cttctctccg
    24901 gagggggtct gagcatggcc tctcccgttc gaattttggt ggtggatgac gacgagatca
    24961 cctgcaacct cttggaagag gtattgtcca aggagggata tgccgtggac aaggccctga
    25021 acgggcagga ggcgatcgaa aagggagaaa aggagtccta tggagttgtg ctcagcgata
    25081 tccgaatgct tgatgtggac ggaatggagg tcctgcgcgc ctaccggcag agaagtccgg
    25141 agaccatcat catcatgatg accgcctttg gttcgatcga aaccgccatc cgggcgatta
    25201 aagaaggggc ttacgactat gtgagcaagc ctttcaagct cgacgaaatc aagctgacca
    25261 tccaaaggtc tctggagcag aagcgcctgg tagaagaaaa catccactac cgccaagagt
    25321 tgattaccaa atataaattg gagaacattg tggggcgcag cccgcagatg ctccaggtct
    25381 ataaaaccat cgcccgcgtg gctgagagcc gctccacggt tttgatcacc ggggagagcg
    25441 ggacggggaa agagctgatc gcccgagcca tccatttcaa cagccccagg tccccccggc
    25501 ctttcgtggc tgtggactgc agctctctag cggaaagcct tctggaaagc gagctcttcg
    25561 gccatgtccg gggagccttc accggagcgg tcacgaccaa aaaaggcctc ttcgaagagg
    25621 ccgatcgcgg gacctgcttt ttggatgaaa tcagcaatat cagtctagcc atacaggcta
    25681 agcttctccg agtcctccag gagcacgaga tcaaacgagt ggggggcacg gagaccatca
    25741 aaatcgacgt gcggatggta gccgccacga accagaacct ggaagagctg gtggcggaaa
    25801 agagatttcg ggaagacctt ttctaccgtc tcaacgtggt ttccatccac cttccttccc
    25861 tccgggaacg cccggaggat attcccttac tagccgatca tttcctgaat aagtatgcca
    25921 ctcaaaacaa caaacctgtc tgtcgaatct cccctgacgc gatggatgtc ttaactcgct
    25981 accgctggcc cgggaatgta cgggagctgg aaaacgtgat tgaacgggcg gcaacccttt
    26041 ccaacaccaa cctgatcatt cccgcagacc tcccccggag gcttcaagta gagctatccc
    26101 agaatgccat cagttccctc ccttccagcc ttcctctttc tgaactggaa aaactctaca
    26161 ttcaaaaggt cctggaggag actgggggaa acaagaagaa ggcggcggat attttaggga
    26221 ttgaccgccg gaccctttac cggatggcta cccgcctggg tctccccatt aaagggtaag
    26281 tgcggcatta tgcctcactt tgcattcctg tcacatttca ccaaccattt aaaatcttat
    26341 atttttctgt aaaatcataa cttgtgtggc gttcttgtct ggaattactc ctggcccaca
    26401 atttttatcc cctttatccg aaacagaaaa tgattcataa ccatttgatt ttaaaatgta
    26461 attaattaat ttgaataatt ccatggactt ttgtcgtcag cggtactgat cttgcttaag
    26521 ggaaaacacg tataaaaatt acaacaaaag acgcaatgaa atataatcca cttcaaaggc
    26581 gtttgggctc ttggtcctta gtagttatta tcctgcttgt ctttgcaggg tgcagcagtc
    26641 tgaaatctga aaacgaaaag ctgaaagaag agatcgtcga tgtaagcgcg gagaacgaga
    26701 aattaagaaa agagctgaac accctcaaat cggagaattc caagatgcat atgcgggtgg
    26761 ctcagctgca catggagatt gcggcccttt atcaggaaat ccagaacatg cagaaggacc
    26821 tggatgcctt aaaagcacag ctgaagggcg gaggaagaaa taaaaagtcg tagcgacgag
    26881 tgatctattt cctctcctct gggggagttg gggattttaa gcaaaaggag gtgaaaaaag
    26941 atgaagaaac tgattgctct tttggtgtgc gttctgtttg ccatgaccct tatggtggct
    27001 gcttgtgcca agaaggagga gcccgcgaag cccgctgagc cggctaagaa ggaagaggtg
    27061 aagccggccg agccagccaa gcctgcggag gcagcaaagc ccgctgaacc ggcgaagccc
    27121 gcggagaagg ctcccgaaaa gaagtaatct cagaggaatt tctttgggcc caaaacccaa
    27181 aaaatctaac cggaaaggag gtgaaagaga atgaaaagaa ttctgattct gatggtggcc
    27241 ctgttgacca tagtggcctt cgcaggcgga gtgatggcgc aggctccggc aaagaccgag
    27301 aaacccgctg ctccggctcc tgaaaaggca gctcccgcgc ccgctcccaa accggcagcg
    27361 gaaaagccca aagccctaaa ggcaacggga gccgttgcgg catacgaggc cgggaaaatg
    27421 atcgcggtga aggtgaagga caaggagatg aagtttgaca tcgccgccga cgccaaggtc
    27481 caaggagagg tcaaggaagg cgccaaggtg accgtaacgt acaaaaagga catggacaaa
    27541 atggttgcca cttctatctc cgggccgaaa ccgaagaaag aagctccacc caagaaggct
    27601 ccggaaaagg ctccggaaaa gaagtagttc ttttccaact ggcgaaaaga ggcataagcc
    27661 ctcaaaaggg ttatgcctct tttttttacc ccccccgttg ggctgttttt tccctgccat
    27721 gcagggaggg tggcttaata agctcagatg caagtgccag aaagcgtccg gggaaattct
    27781 caggtgatag gcgaagcggc atttcgttgt ctccaggaat ccgaatccat agccaaaatt
    27841 aattccaaaa aagttcataa aagagcttga caataattta agcgctatgc tatgaatcct
    27901 tgaatgaata atcattcatt ttagtcatct gggatcaagt tcttaaataa aactcaatag
    27961 ataaccgaaa ggaagggtca tgagaagcag aaaaaataac aaaaaccgat tactgatcct
    28021 gattaccggg atattccttc tccttctggc cacgccgctc ctggccagcg aggcagagct
    28081 ggaaatcccc gtcctcaccg attctcaaaa cacccttctc atggttgggt ttttaatatg
    28141 tttcctggga atggctttcg gggcctacga atatgtaaag gtgaaagcgt tcccggccca
    28201 caagtccatg ctggatgtag ccaatgtgat cttccagacc tgcaagacct acctgctcca
    28261 gcagggaaaa ctcctcatca tcctggagat ttttatcggg gtgtgtatcg cttactactt
    28321 tggcttcctg cagcacatgg acgcgggcgg ggtgatcctt attcttatct ggtccgtggt
    28381 gggaatcctg ggctcctaca gcgtggcctg gttcggcatc cggatgaata ccttggccaa
    28441 ctcccggatg gcttttgcct ccctggaggg gaagccgatt aaactgctgg acattcccct
    28501 gcgggccggg atgagcatcg gcgtccttct ggtgtgcgtc gagctcatca tgatgctcat
    28561 tattctggtt ttcatgcccc gctccaccgc cggcgcctgt tttatcgggt ttgccatcgg
    28621 ggaatccctc ggcgccagcg cgctgcgcat ctgcggcggt atcttcacca agatcgcgga
    28681 catcggctcc gacttgatga agatcgtctt caagatcaaa gaagacgacc cgagaaaccc
    28741 gggggtgatt gccgactgca ccggggataa cgcgggggac agcgtgggac ccaccgccga
    28801 cggtttcgaa acctacggcg tgaccggcgt ggccctcatc agcttcatcg tcctgggggt
    28861 cttcgagcag ttccgggccg agctgatcac ctggatcttc gtcatgcgga ttctgatgat
    28921 cctcacttcg atcgtcgctt ttcagttcaa caaggcggtc accacggcca ggttcgcggg
    28981 caagaccaag gtggacttcg aactctccct gaccatgctg gtctggttca cctccattct
    29041 ttccatcatc gtaacctttg ccgccagcta ctggcagatc gggcacctgc ccaataacct
    29101 ttggtttaac ctatccatca ttatcagctg cggaaccatc gctgcggcgc tcattcccga
    29161 actggtgaaa gtcttcacca gctcgaaatc catccactgc cgggaagcgg taacctcagc
    29221 ccgggagggg ggagcctccc tggatatcct gtcgggtttc gtggccggaa atttcagcgc
    29281 cttctggctg gggatggcca ttttcctctt aatgtccgtc gcctattttg ccagcctaaa
    29341 cggcctggat aagttcatgg aatactcctc catcttcgcc ttcgggctgg tggccttcgg
    29401 cttcctggga atggggccgg tgaccatcgc cgtggacagc tacggcccgg tcaccgacaa
    29461 cgcccagtcc atctatgagc tctccctgat cgagaccatt cccaacgtgg aaacggaaat
    29521 ccagagccaa ttcggtttca agcctaactt cgaaaacgcc aaacacttcc tggaagaaaa
    29581 cgacagctgt ggcaacacct ttaaagccac ggccaaaccg gttctgatcg ggaccgcggt
    29641 ggtgggcgcg accaccatga tcttctccct catcctgctc ctgcgcaaga ccctggggat
    29701 ccagccggag accattttga acctgctcaa ccccttcacc ctcctggggt tcctgtgcgg
    29761 gggagcggtg atctactggt tcaccggagc ctccacccag gcggtgacca ccggcgccta
    29821 ccgggcggtc cagtacatca agaagaacat caagctggat gacagcgcct ccctgagcgc
    29881 ctcaacggaa aagtcgaaag aagtggtgaa gatctgcacc caatatgcac agaaggggat
    29941 gttcaacatc ttcatcacca tcttctgctt cgccctgggc ttcgccttct tttccgcgcc
    30001 gggcagcagt aatgccccgg catcgttttt tatcagctat ctgatctcca tcgccgtctt
    30061 cggcttatac caggccattt tcatggccaa cgcgggcggc tgttgggaca acgccaagaa
    30121 ggtggtggaa gtggacctga gagagaaggg aacccccctt cacgcggcga cggtcatcgg
    30181 agatacggtg ggagacccct ttaaggacac ctcctccgtg gccctgaacc ccatcattaa
    30241 gttcaccacc ctcttcggcc tcctggccat ggagatcgct atttcaccca tgatgaaagg
    30301 gattgctcac tacgtgggag tggtgttctt cctcatcgcc ctggtctttg tggcgcgctc
    30361 cttctacggg atgcgaattc cgaaagagta ggagaaggtt ttaaccatga gcccccaagg
    30421 ggtccttccg ggccccaagg gggcttctct ttggagggtt ccgctcctga tccccctcgt
    30481 tttcgtttga ttcttttccc cctgatcgtt ataatctagc cagtctttcc gataatacat
    30541 aagagaccct tctccccatc ccctaggagg ttggtatgca tatcaaaaag ttgtattttt
    30601 ctgtccttct cttgctccct gcaactctcc tgctggcgcc cggagggatg gctctggccc
    30661 aaaccatcac cccggcccat gaacaggagt tcaccgatgc caaaaacgcc atcgacgccg
    30721 cccaaaaggc ccaagcggaa aaatatgctt cggaatccct gaagcaggcg caggaccttc
    30781 tggtggccgc gggcaatgcc cggagctcca aggatgcggt caaatttacc cagaattcga
    30841 ggctggcccg ggcatatgcc gaactggccc gcgccaccgc cgaactgaag accgaggaag
    30901 acaagctggc ggctacccag gacgaactgc agaaagccag ggcggaaatc gaaagcctga
    30961 aaaaagccca gtagcgagga ggaaaagacg atgaaaccaa gcgtttccca cagaatacaa
    31021 attctgtttc tccttttcct gggttatctc ctggtggcct accagaactt gaatcgttcc
    31081 caagttacct ccgaggggct ggggccctcc cagcccgtgg ccaatgatgc tacagaggcg
    31141 gggcgtgccc tgaaccggag ggtggaaatc gccgtccaga ccggagaatg aaagaaaacc
    31201 attgcggatt tcaggttgca gatttgaaat tttaagagat tgctgctggc aatccaaaat
    31261 ctgaaatcag ggtgactcta tgcccgagtt gagacaagac ccggccacca agcagtgggt
    31321 gatcattgcc actgagcggg ccaagcgacc ccacgatttc agcagacccc cggtccaagt
    31381 ttccgtcgag gagtataaag cggactgtcc attctgcctc ggcaatgaaa gtttgactcc
    31441 tccggagatc atggcttacc gcaaaggagg gccggccaac ggcccgggtt ggtgggttcg
    31501 ggcctttccc aataagtttt cagccctggt cccggaagga agtctgtccc gcaaagaaga
    31561 agagggaatc ttccgaaaga tggacggagt gggacagcat gaagtcattg tggaatgtcc
    31621 tcaacacaac ctgtgcattc ccctgatggc cgacaagcag gtcgaagaga tcatcctcgc
    31681 ctaccgggaa cgatacctgg ccctgaggga ggacccccgc ttcaagctga tcatcatctt
    31741 taaaaatcac gggtccaagg ccggcacctc cctggaacac ccccattccc agttggtggc
    31801 catgcccatc gtccccctta ccatccgcta tcgttttgaa aaggcctaca cccatttcga
    31861 cgatgaaggg agctgcgtgt attgcgacgt tttgcgggag gggtttaagc aacccacgcg
    31921 cctggtcctg gagaccgacc gattcgccgc ttttcacccc ttcgcttccc gtgcgccttt
    31981 cgaaacctgg atcatcccca aggcgcacaa tgcttctttc ggttcgattt ccatggacgg
    32041 ggctaaagag tttgcttacg tgctcaaaaa gacgctgggg aagttatacc ggggattgaa
    32101 taatcccgac tataactaca ttatccatac cgcccccatt aaggacgagc aggaagaata
    32161 cttccactgg cacctgcagg tcctgccccg cctggtgacc cccgccgggt tcgagcttgg
    32221 aaccggcatg tttatcaacg tcgctctccc cgaggagacc gcggcttata tccgcaacgt
    32281 gccggattga tattctcccc caggagccta gggatatgga agggaggaga ccccggtggg
    32341 tcctttttgc cattacctgt cgtacaggtc gaaaaattta atgtactttt ccaggagaag
    32401 gttccggtcg aattcccaga actgcttcac catctcctcc tgctcccgct tgctaaagta
    32461 ttccagggag ggagggaaaa aggctttctc
//
LOCUS       DASPRR010000030         9628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1300259, whole genome shotgun sequence.
ACCESSION   DASPRR010000030 DASPRR010000000
VERSION     DASPRR010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300259"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2268
                     /locus_tag="VEL68_01155"
     CDS             <1..2268
                     /locus_tag="VEL68_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HXZ34605.1"
                     /translation="EKKGLTFKDAAVRNRFAVTGKQNLLRLRPAALAVPELASLIEVG
                     NFEDHLSRLAEVDWIVEVVVENLKIKQELFKKVAAVHKPGTIVSTNTSGIPLKDICAG
                     MDPEFKQYFLGTHFFNPPRYMKLLEIITLPETKPEVVKFMAEFGEKVLGKGIVYAKDT
                     PNFIANRIGIFGMLYLMKAMVEDGYSIEEVDAITGPAMGRPRSAAFGTTDLVGLDTFA
                     HVARNIYENVPKDEMREIFKAPDFVQKMLEKGWLGNKSGQGFYKRVKSEAGKEKLVLD
                     YKTMEYRPSQKVKYPSLDAAKAVSGAAKAKALIYAEDRAGQFAWKITNESLLYTAKRI
                     PEIADDVYNIDNAMKWGFNWDAGPFEAWDALGVEESVARMKKEGKPIPLLVRKLLAAK
                     GKSFYRRKNGQVSFFDLKTGKYKKVAEKPEIILLPSLKERKKEVKSNAGASLVDMGDG
                     VACLEFHTYMNAIGQEIIEMIHESLKIVEKDFVGMVIGNHAERFSVGANLLMLVGEIA
                     KSNWAGIDAAIRAIQDAMMAMKYFEKPIVAAPHGMALGGGCEVCLSSHRIVAALETYM
                     GQVEIGVGLLPGAAGNKEVYLRCIEGIPDGVSVDLLPFLRKAFENIAMAKVSTSAEEA
                     RKLSFLRPTDRVVANGDRLLYEAKQTVLAMIQEGWKAPRPKLIPVAGDGGRAAIKYMA
                     NTMSQGGFITPYEEFIAGKLAYILTGGDVLSGAMVTEQKMLDLEREAFVSLCGEKKTQ
                     ERITAMLKTNKPLRN"
     gene            2281..3468
                     /locus_tag="VEL68_01160"
     CDS             2281..3468
                     /locus_tag="VEL68_01160"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXZ34606.1"
                     /translation="MKEAVIVSAVRTAVGRAPRGSLRNTRPDDMAGWVVKEAMNRVPG
                     LKPEEVDDVVIGCSFPEAEQGLNVARLINYIAGLPYKVPGQTVNRFCSSGLQAIAIGA
                     EHIMCGFADVIIGGGVESMSMVPMGGNKTVPNLKLIERYPEGYTAMGVTAENVAVKFG
                     IKREDQDAFALKSHQKAAAAIKAGRFKDEILPIKVKTQEVSEDGQVKFREFVFDTDEG
                     VRADSTLEALAKLRPAFSTTGSVTPGNASPLNDGAAATVILSKEKAKALGVKPMATFR
                     SFAVAGVPPELMGIGPVEAIPKALKLAGVTLDQIDLFELNEAFASQALYCCRTLGLNM
                     DKVNVNGGAIALGHALGNSGARLTTTLMHEMKRRNVRYGVVSMCIGFGMGAAAVFERE
                     PNY"
     gene            3541..4497
                     /locus_tag="VEL68_01165"
     CDS             3541..4497
                     /locus_tag="VEL68_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34607.1"
                     /translation="MGTSFLWIAVLLFMVWGESPTLRAQTLPSAQQLLPLAPHLSLQE
                     KDLRSFRTTGRLDWQQVHLKFELCAEKPDRTALRVLDSRDHTPILWASGREFLFYDPL
                     EDEVVMGKGVPSFHLRMEKESNPEREEDRGKGNLILEFGISTDVTKASSGLSVDLKSF
                     WMNAEGVPEVRAGKEGTLLLNGRTPRGGRIVAHIHPSRDAGSYKRFELFIAEGAKEPF
                     CTLEEITFNQPSKPGQFIFPKRFLVNSGLPVRQIEPDKTGFSLDMGRLVRAMLARMAI
                     YGAEEVKPLVEKLYSQKPDWGALAMRDRRASEVLISMTEERE"
     gene            4604..5386
                     /locus_tag="VEL68_01170"
     CDS             4604..5386
                     /locus_tag="VEL68_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXZ34608.1"
                     /translation="MDESRMKTLADMLLSAERSRKPIAPLTESDRGITVDEAYRVQLL
                     MMDMKKSSGQVVVGKKIGITSRAMQVMLGVSEPDYGHILDGMVVAEGEMIRTADLIQP
                     RVEGEIAFILKEDLEGPGVTLADVIRCSEGVIPALEVIDSRIVDWKIKLPDTVADNAS
                     SARIVLGGKITPVLGLDLQTVGMVLEKNGEIAATAAGAAVLGHPAQAVAWLANKLATY
                     RISLAKGEVILSGSLTAAVPVAAGDFIRADFGHLGDVKIKFA"
     gene            complement(6002..6721)
                     /locus_tag="VEL68_01175"
     CDS             complement(6002..6721)
                     /locus_tag="VEL68_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34609.1"
                     /translation="MLLSAENITSGYVEEIDILRDLSLEVREKAITGIIGPNGAGKST
                     LMKTIYGFLHPRRGRILYEGNEIQTKPPHQLKRMGISYMLQEYSTFPQLTVKDNLLLG
                     AWIYRKDAKLIKRRLEEVFEFFPMLRDKRREKANYLSGGFLRMLSIGKETITKPKLFL
                     VDEPSSGLAPIIVKEIYNFLIKIVEEGTTLLLVDQNIRKALEVASYMYLFEMGQVKKK
                     GPKEDFEESIRQIIKESLFTR"
     gene            complement(6728..7462)
                     /locus_tag="VEL68_01180"
     CDS             complement(6728..7462)
                     /locus_tag="VEL68_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34610.1"
                     /translation="MDGREKQILRLENVVRDFGGLRAVNHVSLQVEEGEVRGLIGPNG
                     SGKTTLINLITGIYKITEGDIYFGAKRISGLQPDVIASLGIMRTFQVPRVFRDMTVLE
                     NMMVPALSQGQTKQEAKTRAEELLEFALLTRLKNEPAKSLSGGQSMLLQIVRVFMNKR
                     LMLYVMDEPFAGVHESIKGIILKTIKAMNQEKGITFLIVSHEMTTISNLCPRVSVLYR
                     GGIISQGTMEEIANDPLVIDAYLGGQ"
     gene            complement(7517..8479)
                     /locus_tag="VEL68_01185"
     CDS             complement(7517..8479)
                     /locus_tag="VEL68_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34611.1"
                     /translation="MQTFKKYSVLSVLLLISLLLPHFITDYWIYVLTVAFYYAIMAAG
                     WNLLGGYTGQFSLCHSTFALIGAYASALTIVKLGLPLWVGILSGTLLTLVLSLGLGIV
                     CLRVRGIYLALITWAFAEVIRTFIRVAYEFTGGDRGLNSPYLFRSVKPLPYYYLFLGL
                     TVLAIILIAVVMRSRIGFYLRAIRNDEVAARAMGVNTVRWKIFAFMLPSGLAGVTGAF
                     YGHYIGLISPVLGEFNEMAMIIIFVVIGGMRTQAGPILGAISIRYLMEFLREWAEIRI
                     VILSAIVILIMRFFNGGLMEFLRRAGGWVRKKDAVSAYASMGEK"
     gene            complement(8571..9530)
                     /locus_tag="VEL68_01190"
     CDS             complement(8571..9530)
                     /locus_tag="VEL68_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34612.1"
                     /translation="MLEAPRKVILRGALKSLLKENRAIDLHLFSQYLIAGLIMGVLYC
                     LMSLGINFIYGIMKVINWAMGEYYMIGSYVLYLLVTFPLGVKYWYVAVLAAAIAVFFL
                     GAFVQVLLIKPMFTGAIGERGEYGTIVTIATAIFIRNLAVVIGGPYIYTPPDYAGPTN
                     IGPLPISGNTLVALMGALILLSLFYYLIKRTWVGRALQGVAQNRIGIQTSGVNILKFD
                     MLAFGIGTALAAAAGALLAPVYLVYPTCGLVSSVRGFEIIVIAGLGSIWGVLVASLAL
                     GVVESLGAVFISPSFMDIYGFVLLVIVLSLKPYGLFGKEERIA"
BASE COUNT         2370 a         2579 c         2673 g         2006 t
ORIGIN      
        1 gaaaagaagg ggctcacctt caaggatgca gcggtgcgga accgcttcgc cgtgacgggg
       61 aaacagaacc tgcttcgcct gaggcccgcc gccttggccg ttcccgaatt ggccagcctc
      121 atcgaggtgg gcaacttcga agaccacctc tcccgcctgg cggaggtgga ctggatcgtc
      181 gaggtggtag tggagaacct gaagatcaag caggagctct tcaaaaaagt agccgccgtt
      241 cacaagccgg ggacgatcgt gagtaccaac acctcaggga ttcccctaaa agatatttgc
      301 gccggcatgg acccggaatt caaacagtac ttcttgggga ctcacttttt caacccgccc
      361 cggtacatga agctcctgga gatcatcacc ctgccggaga ccaagcccga ggtggtgaag
      421 ttcatggctg aattcggaga aaaagttctg ggcaaaggaa tcgtctatgc caaggacact
      481 cccaacttca tcgccaaccg gatcgggatc ttcgggatgc tctacctgat gaaggccatg
      541 gtggaagacg gctacagcat cgaagaagtg gacgccatca ccgggccggc catggggcgg
      601 ccgaggagcg ccgccttcgg gaccaccgac ctggtgggac tggacacctt cgcccacgta
      661 gccaggaaca tatacgaaaa cgtccccaag gacgagatgc gggagatttt caaagccccc
      721 gattttgtgc agaagatgct ggaaaagggc tggctcggga ataaaagcgg ccaaggcttt
      781 tataagcggg tgaaatcgga agccgggaaa gagaagctgg tgctggatta caaaaccatg
      841 gaataccggc cctcgcagaa ggtcaaatat ccctccctgg acgcggccaa ggcagtctcc
      901 ggcgcggcca aggctaaagc cctcatctat gccgaggacc gggccgggca attcgcctgg
      961 aagatcacaa atgaatccct gctctacacg gccaaaagga tccccgagat cgccgatgac
     1021 gtgtacaaca tcgacaatgc catgaagtgg ggtttcaatt gggatgcggg ccccttcgaa
     1081 gcctgggacg ccctgggggt cgaggagtcg gtggcccgga tgaaaaaaga aggaaagccc
     1141 atcccactcc tggtgaggaa gcttctggcg gccaagggga aatccttcta ccggaggaaa
     1201 aacgggcagg tctctttctt tgacctgaaa acggggaagt ataaaaaggt cgcggagaag
     1261 cccgagatca tcctcctgcc ttcgctgaag gaacggaaga aagaggtcaa gtccaacgcc
     1321 ggggccagcc tggtcgatat gggagatggc gtggcctgcc tggagtttca cacctacatg
     1381 aacgccatcg gccaggagat catcgagatg atccacgagt ctctcaagat cgtggagaag
     1441 gactttgtgg gaatggtcat cggcaaccat gcggagaggt tttccgtggg ggccaacctg
     1501 ctcatgctgg tgggggagat cgccaaatcc aactgggcgg ggatcgatgc agccatccgg
     1561 gccatccagg atgccatgat ggccatgaag tatttcgaga aacccattgt ggccgctccc
     1621 catggaatgg ccttgggcgg cgggtgcgaa gtctgcctgt ccagccatcg cattgtggcc
     1681 gccctggaga cgtacatggg ccaggtggag atcggagtgg ggttgctgcc gggggctgcc
     1741 gggaataagg aagtttacct ccgctgcatt gaagggattc ccgacggggt gtccgtagac
     1801 ctccttccct tcctgcggaa ggccttcgaa aacatcgcca tggccaaagt ctcgaccagc
     1861 gccgaagagg cccggaaact ttccttcctg cggcccacgg accgggtggt ggccaatggg
     1921 gatcgtctcc tgtacgaggc caagcagacc gtcctggcta tgatccagga agggtggaaa
     1981 gcgccccggc ccaagctcat ccccgtggcc ggagacggcg ggcgggcggc catcaagtac
     2041 atggccaaca ccatgagcca gggggggttc atcaccccat acgaagagtt catcgccggc
     2101 aagctggcct acatcctcac cggcggagac gtcctgagcg gagccatggt cacggagcag
     2161 aagatgctgg acctggagcg ggaggccttc gtcagcctct gcggggagaa gaagacccag
     2221 gagcggatca ccgccatgct caagaccaac aaaccgctca ggaactagaa ggaggaaacc
     2281 atgaaagaag ctgtcatcgt ttctgccgtt cgcaccgcgg tgggccgggc tccccggggg
     2341 agcctgcgca acacccggcc ggatgacatg gcgggctggg tggtcaaaga ggccatgaac
     2401 cgggtccccg ggctgaagcc ggaagaagtg gatgatgtgg tcatcggctg cagcttcccg
     2461 gaagccgaac aggggctgaa cgtggcccgt ctgatcaact acattgccgg ccttccctac
     2521 aaggtacccg ggcagaccgt aaaccggttc tgttcctccg ggctccaggc catcgccatc
     2581 ggcgccgagc acatcatgtg cgggtttgcc gatgtcatca tcggcggggg agtggagtcc
     2641 atgtccatgg ttcccatggg cgggaacaag accgttccca acctcaagct gatcgaaagg
     2701 tatcccgagg gatacacagc catgggggta acggcggaga acgtggccgt gaagttcggg
     2761 atcaaacggg aagaccagga cgcctttgcc ctgaagagtc accagaaagc ggcggcggcc
     2821 atcaaggccg gccggttcaa ggatgaaatc ctgcccatca aggtgaagac ccaggaagta
     2881 tccgaagacg gccaggtaaa gttccgggaa ttcgtcttcg acaccgatga gggggttcgg
     2941 gcagactcca ccctggaggc tctggccaag ctgcggccgg cattctccac cacgggatcg
     3001 gtcactccgg gaaacgcttc tcccttaaac gacggggctg cggccacggt catcctgtcc
     3061 aaggagaagg ccaaagctct gggagtgaag cccatggcta ccttccgctc ctttgccgtg
     3121 gccggcgttc cgccggaact catgggcatc gggccggtgg aggccattcc caaggctctc
     3181 aaactggcag gggtcaccct ggaccagatc gacctattcg agctgaatga ggccttcgcc
     3241 tcccaggccc tgtactgctg ccggaccctg ggcctgaaca tggacaaggt gaacgtgaac
     3301 ggcggggcca ttgccctggg ccatgccctg ggcaacagcg gggcccggct gaccaccacc
     3361 ctgatgcatg aaatgaaacg ccgcaacgtc cgttacgggg tcgtctccat gtgcatcggc
     3421 ttcggcatgg gcgcggccgc ggtcttcgag agagaaccca attactgaag aaggctcatc
     3481 tacccgtgac caagggcagg cctttgggcc tgccctttta agtggcggag gtctgcgggc
     3541 atgggaactt catttctctg gatcgccgtc ctactgttta tggtttgggg cgaaagtcca
     3601 accctccgcg cccagacctt gccctcagcc caacagctct tacccctggc accgcatttg
     3661 tcgctccagg aaaaggacct acgctctttc cgaactaccg ggcggcttga ttggcagcag
     3721 gtgcatctga agttcgagct ttgcgccgaa aagcccgatc ggaccgctct tagggttctg
     3781 gactccagag accatacccc gatcctctgg gcctccggga gggaattctt gttctatgac
     3841 cccctggaag acgaggtggt catgggcaag ggggtgcctt cttttcatct gcgcatggag
     3901 aaagaaagca acccagaaag ggaagaggat cggggcaagg gaaatttgat cttggagttc
     3961 ggtatttcca ccgatgtgac caaagcgtca agcggcctat cggtggactt gaagtccttt
     4021 tggatgaacg cggagggcgt ccctgaagtg cgcgctggca aagaagggac tttgctcctg
     4081 aatggaagga ccccgagagg cgggcggatc gtggcccaca tccacccctc ccgcgatgcc
     4141 ggctcctaca aacggttcga actattcatt gccgagggcg cgaaggaacc gttctgcacc
     4201 ctcgaagaga tcaccttcaa ccagccctcg aagcccggcc aattcatttt tcccaaaaga
     4261 ttcctggtga attctggcct ccctgtgcgc cagattgagc cggacaaaac cggattttcc
     4321 ttggacatgg gaaggctggt tcgagccatg ctggcccgca tggccattta tggcgcggaa
     4381 gaggtaaaac ctcttgtgga aaagttgtat tcccagaagc ccgattgggg ggcactggcg
     4441 atgcgggaca ggcgtgcaag tgaagtcctt atttccatga ccgaggaaag ggagtaagaa
     4501 tcgaggggaa gcctaagggg ggttcctgga gaaagtgcat tgcctaaaaa ccgattccca
     4561 aggcaatctt attaaaaaac acccataaac gaaaggaacg cccatggatg agagtcggat
     4621 gaagacgctg gccgatatgc ttctctctgc ggagcggtcc cgcaagccca ttgcacccct
     4681 cacggagtct gaccgaggaa tcaccgtgga tgaagcctat cgggtccaac ttcttatgat
     4741 ggatatgaag aaatccagtg ggcaggtggt tgtggggaag aagatcggaa ttaccagtcg
     4801 ggccatgcag gtcatgctgg gagtgagtga accggattac gggcacatcc tggatgggat
     4861 ggtggtcgcg gaaggagaga tgattcgaac ggcggatttg attcagccgc gggtcgaggg
     4921 ggaaatcgcc ttcatattga aagaagacct ggaaggacca ggggtgacgc ttgccgacgt
     4981 cattcgctgc agcgaggggg tgattcccgc cctggaggtt atcgacagcc ggattgtgga
     5041 ctggaaaatc aagcttcccg acactgtggc cgacaatgcc tccagcgcgc ggatcgtcct
     5101 gggaggcaaa atcaccccgg ttttggggct ggacctgcaa accgtgggca tggtactgga
     5161 aaaaaacgga gaaattgcgg cgactgccgc aggggcggcc gtcctcggac atcccgccca
     5221 ggccgtggcc tggctggcca acaaactcgc aacgtacaga atttccctgg ctaaagggga
     5281 agtgattctt tcggggtctc tgaccgcggc agtcccggtg gctgccggcg atttcatccg
     5341 tgctgatttc ggccatctgg gggacgtgaa gatcaagttt gcctgaggga ctgaagacgg
     5401 aaaatccttt taccgtccag ggctttcctc aggactgggg gctcgatcac ctttgttgtt
     5461 ctttcccaga ttcttagctt tcagactcgg cactattttg gcacttatct tttcaggagg
     5521 aacagggatt tgcggctttg ccaggaactc ttgggccaca gtatctctgg aatcacgagt
     5581 cgttatgctt atatgcttga tcgggagagg caaaaagcgc atatccatgt ttcgcgaatg
     5641 aggtttaaat agtgacaatt aatgtcgcta aaataataga gattgtcaat aacctggaag
     5701 agtcctgtaa agctcttgac tgcatcaagg cgaatttgga atcaacatgg ccacatccat
     5761 tagtggatga atattaccaa attttgtttg ccctaacccg ggccaccgaa gcgaaagaga
     5821 gattggagga actcgtcgaa aaaagaagga ttaagaaggg agaatattct ttcctttgcc
     5881 ctgggtaatt attttatctt ataacctttt gcgcagaagg gattcggact ggttttcttg
     5941 gagggtctaa gccgcagaag cccgcaggat aaaagcagct gaggtaccga tgtcttcaat
     6001 actacctggt aaagagcgat tcttttatga tctgccggat actctcttca aaatcctctt
     6061 tgggtccctt ctttttgacc tgccccattt caaagagata catgtagctc gcaacttcga
     6121 gggcttttct gatattctgg tcaacgagga gcagggtggt accctcttcg acaattttaa
     6181 tcaaaaagtt ataaatttct tttacgatga tcggtgccag acccgaagag ggttcatcga
     6241 ccaagaataa ctttggtttg gtgatcgttt cctttccgat agaaagcatc cgtaaaaagc
     6301 ctccactcag gtaattggct ttttcccttc ttttatcccg aagcatggga aaaaattcaa
     6361 aaacttcctc cagtctcctt tttatcaatt tcgcatcctt ccgataaatc catgcaccta
     6421 aaaggaggtt atctttaacc gtaagttgag gaaaagtact gtactcctga agcatgtaac
     6481 ttatccccat tcgtttcaat tgatggggag gtttggtttg tatttcattg ccctcataca
     6541 ggattcttcc cctgcgggga tgaagaaaac cgtagatcgt cttcatcaaa gtagatttcc
     6601 cagccccgtt cgggccaata atcccggtaa tcgctttttc cctgacttcc aaggagaggt
     6661 ctcttaggat atcgatttct tccacatagc cggaggtaat attttcagcg gataatagca
     6721 tcgggtatca ttgtccccct aagtaagcat caatgaccaa agggtcgttg gcaatttctt
     6781 ccatggtacc ctgagaaatg attccccccc tataaagaac ggagactctt gggcatagat
     6841 tgctgatcgt cgtcatctcg tgactgacaa tcaaaaaagt tattcctttt tcctggttca
     6901 tcgctttaat ggtcttgagg atgatcccct tgatggactc gtggaccccg gcaaagggtt
     6961 cgtccattac gtacagcatc agccttttat tcatgaacac tcgaacaatt tgaagcagca
     7021 tgctttgccc accggaaaga ctcttggcgg gttcgttctt tagccgggtc aagagggcga
     7081 attcaagaag ctcttcagca cgggttttag cctcctgctt cgtttgccct tgagataagg
     7141 ccgggaccat catgttttcg aggacggtca tgtcgcggaa aactcgggga acctgaaagg
     7201 ttcgcatgat ccctaaggag gcgatcacgt cgggttgcaa cccgctaatt cgcttcgcac
     7261 cgaaataaat atccccctcg gttattttat aaatacccgt gattaggttg atgagagttg
     7321 tttttccgga accattgggc ccaataagac cccggacctc cccttcctca acctgaagag
     7381 agacatggtt gaccgcccgc aatccgccga agtctctgac tacgttttct aacctcaata
     7441 tctgtttctc tcggccatcc atctctatcc tatgcactcc cgaagaaccc ttccagatca
     7501 acgccctgtt gaaagatcac ttttccccca tcgaagcata agccgaaacg gcgtctttct
     7561 ttcggaccca tcccccggcc cgcctgagaa actccatgag cccaccattg aagaatcgca
     7621 tgattagaat gacaatggcg ctcaggatca cgatccggat ttcagcccat tcccttaaaa
     7681 attccatgag ataacgaatg gagatggccc cgaggatggg ccccgcctgg gttcgcatac
     7741 ccccaatcac cacgaaaatg ataatcatcg ccatctcatt aaattcgccc aatacgggac
     7801 tgatcaaccc gatataatgt ccatagaacg cccccgttac cccggccagg ccgctaggga
     7861 gcataaaggc aaagattttc catcgaaccg tgttcacccc catggcccga gcggcaacct
     7921 cgtcattgcg gatggctcgc aggtagaagc caatccggga ccgcatgacc accgcaataa
     7981 gaatgatagc cagaaccgtg aggcccaaga acaggtagta gtaaggcaaa ggctttaccg
     8041 atctaaagag gtaaggagaa ttcaggcccc tgtccccgcc ggtgaattca taggcgaccc
     8101 ggatgaacgt tcgaattact tcggcgaagg cccaggttat caaagccaaa taaattcccc
     8161 gaacccgcag acatacgatc cccagcccta aactgagaac cagggttagg agagttccgg
     8221 acagaattcc cacccaaagg ggaagtccca atttgacgat cgttagagcg gaagcgtagg
     8281 ccccgatgag ggcaaaggta gaatggcaaa gagagaattg gccggtatag ccacccagca
     8341 aattccaacc tgccgccatg atggcgtaat aaaaagcaac tgttaaaacg taaatccaat
     8401 aatccgtgat aaaatgcgga agcaggagcg agatcaacaa taggacggag agtactgaat
     8461 attttttaaa ggtctgcatg aatcacttct ggcaaagccg caggaattcg atcccttcga
     8521 tcctggaatc ggcgggagct ttccctcgaa attttgatca ggtttccaga tcaggcgatc
     8581 cgttcttctt tcccgaatag gccgtagggt tttagcgata gaacgattac cagaagaaca
     8641 aacccataaa tgtccataaa agaaggacta ataaatacag cgcccaagct ttccacgacc
     8701 ccaagagcaa gactggccac gagaacgccc cagatggaac ccagtccggc aatcacaatg
     8761 atctcgaacc ctcgaaccga ggaaacgaga ccgcaggtgg gatataccag gtagacgggc
     8821 gctaagagag ctcctgcagc agcagccaaa gccgtcccga ttccaaaggc cagcatgtca
     8881 aatttcagga tgttgacgcc ggaggtttgg atcccaattc ggttctgggc taccccctgg
     8941 agggccctcc cgacccaggt tctttttatc aagtaataga ataaggaaag cagaatcaag
     9001 gcccccatga gagccaccag ggtattcccg ctaatcggca ggggtccgat gtttgtgggg
     9061 cccgcataat cgggcggcgt ataaatgtac gggcccccga taacgacagc cagatttcga
     9121 ataaagatcg ccgtggcaat cgtaacaatc gttccatatt ctcccctctc cccgatcgct
     9181 cccgtaaaca tgggttttat taaaaggacc tggacaaagg ccccgaggaa aaatacggca
     9241 atggccgctg ccaggacggc gacgtaccag tacttcaccc ccaggggaaa agtgaccaat
     9301 aaatacagga cgtaggagcc gatcatgtaa tattcaccca tcgcccaatt gatcactttc
     9361 atgatgccgt aaataaaatt gatccccagg gacatcaggc agtagaggac ccccatgatg
     9421 aggccggcaa tcagatactg gctgaaaagg tgcaggtcga tggctctatt ctcctttaag
     9481 agagacttta aggccccccg aaggataacc ttccgagggg cctcgaggat ttacgcgaaa
     9541 tacggtttcc gctgcccttt cctttcaggg acgctcgaat ttcccatcgc cgccgaactg
     9601 ggggggccag actactttcg tctcggcg
//
LOCUS       DASPRR010000031         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_813695, whole genome shotgun sequence.
ACCESSION   DASPRR010000031 DASPRR010000000
VERSION     DASPRR010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813695"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VEL68_01195"
     CDS             complement(<1..717)
                     /locus_tag="VEL68_01195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXZ34613.1"
                     /translation="MIGWLAPIAGFAVFALNEIANPRWAFLGKVHCKGDPGGVALTFD
                     DGPHPEHTPKVLETLDRFQAKANFFVIGKYLERYGDLTAAASRAGHLVANHSFHHFRA
                     MSFFSPEEIRREVLQCQEELEKWVGYRPRFYRQPAGFRNPKIFGILKEMGMSLVGWQT
                     HAFDTMAKSPGRIAQRILKTFQPGGIILLHDGWDRGGERDRTPTLEALPLILQGLKDR
                     GLQFFTLDRLLGMSKEAVKNN"
     gene            complement(723..1622)
                     /locus_tag="VEL68_01200"
     CDS             complement(723..1622)
                     /locus_tag="VEL68_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXZ34614.1"
                     /translation="MPRKRILHRLAYLYLFLIGFFGHILPRWLMLWFAVLIGNFYWAV
                     MKYDREMVRRNLSHVLDDRSQVGRTVRRLYVRYAKYLVDFTRMGLLKEKHLRRLVLSF
                     EGKEHIDGAIARGKGTLILTAHLGNWEMGGIFLSLMGYSLTVITAPDVEERLHEYRVR
                     LRHEQRIKVVTLYDSLASSLAVLKALQANELVALLGDRDLFGKGVPVRFFGQKVFFPI
                     GPALLSYLSEAVLIPTFVLMERENRYRCIAEPPLDLQRSGNRDKDLAENTQRIAAVLE
                     KFIRNYPDQWYTFYDYFSRHRAP"
     gene            1866..2255
                     /locus_tag="VEL68_01205"
                     /pseudo
     CDS             1866..2255
                     /locus_tag="VEL68_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941622.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          589 a          662 c          686 g          560 t
ORIGIN      
        1 attgttctta accgcttctt tgctcatccc caaaagcctg tccagggtga agaactggag
       61 cccccgatcc ttcaaccctt gaagaatcaa gggaagggcc tccagggtcg gagttcggtc
      121 tctttctcca ccccggtccc agccatcatg gaggaggatg attcctcctg gctggaaggt
      181 tttcaggatt ctttgggcga tccgtccggg gctttttgcc atcgtgtcaa aagcgtgtgt
      241 ctgccatcct actagggaca ttcccatctc ttttaagata ccaaagattt tggggttccg
      301 aaacccggca ggctggcggt agaagcgggg ccggtatccc acccactttt ctaattcctc
      361 ctggcactgc aatacttccc gtcgaatctc ttcgggggaa aagaagctca tcgcccggaa
      421 atgatgaaaa gaatgattgg ccacgaggtg cccggcccgt gaagccgcag ccgtcaggtc
      481 tccgtacctt tccaggtatt tcccgatcac aaagaaattc gccttcgctt ggaaccggtc
      541 aagggtctcc agaaccttgg gagtgtgctc cgggtgaggt ccgtcatcga aagtcagagc
      601 cactccgcca gggtccccct tgcaatgtac ctttcccaga aacgcccagc ggggattggc
      661 aatctcgttg agggcgaaaa cggcgaagcc agcgatgggg gccagccaac cgatcatgaa
      721 aatcagggag ctctatgccg ggagaaatag tcgtaaaaag tataccactg gtcgggataa
      781 ttgcgaatga acttctccag cacggcggcg atccgctggg tgttctcggc caaatccttg
      841 tcccggttcc cgcttcgctg gaggtcaagg gggggctcgg caatacaccg gtaccggttc
      901 tcccgctcca tcagtacaaa agtggggatg aggacggcct cggagagata gctcagaaga
      961 gcgggaccga tggggaagaa aaccttctgc ccgaaaaaac ggaccggaac tcctttgccg
     1021 aacaagtccc ggtctcccag gagggccacc agctcattgg cctggagcgc cttgagaacc
     1081 gcaagcgagg aagccaacga gtcgtacagg gtgaccacct tgatcctttg ctcgtggcga
     1141 agccgtaccc ggtactcgtg aagccgctcc tccacatcgg gggcggtgat gaccgtgagg
     1201 gaatagccca ttaaagatag aaagattccc cccatctccc agtttcccag atgagcggtc
     1261 agaatcagcg ttcctttccc ccgggcgatg gccccatcga tatgctcttt cccctcgaaa
     1321 ctcaggacca ggcggcgcaa atgcttctct ttcaagagac ccatgcgggt aaagtcaacc
     1381 aggtatttgg cgtaacggac gtagagacgc cggacggtgc gaccgacttg agagcggtcg
     1441 tccaagacat ggcttaagtt ccgtcggacc atctcgcggt catatttcat caccgcccag
     1501 tagaaatttc cgattaaaac tgcgaaccag agcatgagcc atcgcgggag gatgtgaccg
     1561 aaaaagccga tcaggaagag gtacagatag gcaaggcggt ggaggattct tttccttggc
     1621 attgttgagg aaattttact ttctccctcg gatcagtgtc aagaaaaatg ctggattgta
     1681 gagaaacttc tgaagaaggt gttgattctc agtttggggg atggtcacaa gtactttctc
     1741 tcaggagaaa gggggataag gttccatcgg attaaaagat actggggagt ggaggattcg
     1801 tcggatagga gacgtacatt agtttataag ctgacatctc tatttatttc tggtattaac
     1861 agaatatacc acgtaaatct cgcatcgatg ctccggaggc tctccatcac atcattgcca
     1921 gaggcattga gcgtagagcc atatttcgag acgatcagga ccgagatagt ttcttggagc
     1981 gtttgggaaa gaacctggtg gaaagccgca ctccgtgtta tgcctgggcg ttgcttcgga
     2041 atcatttcca catcttgctc cgcaccgggg caacgccaat ttccacggtt atgcgtcggg
     2101 tgcttaccgg ctatgctgtt gattttaacc agcgtcatca ccgccagggc catcttttcc
     2161 aaaaccgctt caaatcgatt ctatgtcagg aagacccgta tctcctggag ctggttcgtt
     2221 atatccattt gaatcccttg cgcgcgggaa tcgtcaaagg gatcaagtcg ttaggccgat
     2281 aaatcaaggg ggcaagcccg aggaatttcg aagaatgtcc ccccgggaac ctgagttcaa
     2341 agcaccgtga gccatgagga aagcggcgcc ctcttgggcg gcccttatcg gagccgcccc
     2401 tgatcccggc gccggctacg gaattcggaa acgaacttgc gactcaggac gttaggtccc
     2461 ggaatgaagt cagcggcgct atttgaatca tctttct
//
LOCUS       DASPRR010000032         5928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_656871, whole genome shotgun sequence.
ACCESSION   DASPRR010000032 DASPRR010000000
VERSION     DASPRR010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656871"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VEL68_01210"
     CDS             <1..262
                     /locus_tag="VEL68_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34615.1"
                     /translation="LPGPSGSIFTRRGIRPFSWENNEMNLKKVVTPVKTGVQRLDHPF
                     EQLDSGFRRNDGGKGVLTFSRPSMEPSGTSMIPPEGLGWFME"
     gene            complement(252..1436)
                     /locus_tag="VEL68_01215"
     CDS             complement(252..1436)
                     /locus_tag="VEL68_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HXZ34616.1"
                     /translation="MKPLEIAKNIYWVGSIDWNIRDFHGYSTYQGTTYNAYLIKEEKV
                     VLIDTVKKDFSPEWMDQIRLLIDPKKIDYVISNHAELDHSGSLDWLMHVIGSEKPVYC
                     SKLGAQNLARHFHGGLNLKPVNEGEELKLGQRIVTFLETRMLHWPDSMFTYLKEDQIL
                     FSSDAFGQHYAGMERFDDQIGEAIMPHAQKYFANILLLYSPLILKLVEKVTRMGLPIK
                     MICPDHGIMWRKDPGKIIRAYVQWAQQKPQKKALVIYDSMWHSTQAMAEAIVDALAQE
                     RVDARTMHLRTIHRSDVMTEVLDAGAILVGSPTLNNGLFPTVADFLCYMKGLKPLNKI
                     AAAFGSYGWSGEAVKLINQDLEAMKFRIVDPGLNIQYVPDKKGLEACHALGKKVAQAF
                     TP"
     gene            complement(1570..2421)
                     /locus_tag="VEL68_01220"
     CDS             complement(1570..2421)
                     /locus_tag="VEL68_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXZ34617.1"
                     /translation="MVTLKDYGDYETTWCPGCGNFSVLKAVKQALVACRLRPQDVIFV
                     SGIGQAAKAPHYLNVNLFDGLHGRSLPVATGAKLANPGKTVIVESGDGCNYGEGGNHF
                     LAALRRNIDVTLLVHNNQVYGLTKGQASPTSETGFVTKVQPEGVASSPFNPVAVAIAL
                     RAGFVARGFAGMVDHLALIIQEAIAHRGFSLVDILQPCVSFNKMNTFSWYKSRCKELP
                     PDYDPTNWEVAMKTAEEWGENIPVGILYWNNRPAFESHFPALKEGPLVGKGVDGALLA
                     KILESYR"
     gene            complement(2654..4327)
                     /locus_tag="VEL68_01225"
     CDS             complement(2654..4327)
                     /locus_tag="VEL68_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ34618.1"
                     /translation="MNTLNILIGGEAGQGLVTVGEILAKSLVRAGYYILVTQSYMSRI
                     RGGHNTFSIRVSPDEVIASREEVDLLVALDDLTITLHDKELTPRGRIVADQGCSADEK
                     VCLKVPFGHLASDQFSNIAALGVVSGLMGLKEEVVGRTLEDFFGKKAPGVAEENRKVL
                     RDAMAWTLSQPPFMGKLSPVPNPTRRMMMQGNEAIALGAISAGLKFYSFYPMTPSTSI
                     GQTLAKWAGKMGLVVEQAEDEIAAINMALGASYAGAPSMVGTSGGGFALMVEGVSLAG
                     MTETPVVIVVAQRPGPATGLPTRTEQADLQFVLFAGHGEFPRAIFAPGTVEECFHLTR
                     RAFALAEKYQGPVFVLTDQFLSDSYRSIEPFEVEKLPLAGPSWNAVPASLPYRRFAIT
                     ENGISPRLLPGWSEHRVVAGSDEHTEDGHLTEDLSVRRQMVDKRLRKMEGIRQEVIPP
                     ELKGDGNPEILLVTWGSTKGSVLEVASHLRSKGKKVATLFFPQVWPLVQEQFLPPLQG
                     ARQVISVEGNATGQLAKLIRQESGFEIKKKVGRYDGLPITPEYILRELN"
     gene            complement(4581..5531)
                     /locus_tag="VEL68_01230"
     CDS             complement(4581..5531)
                     /locus_tag="VEL68_01230"
                     /inference="COORDINATES: protein motif:HMM:NF026253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HXZ34619.1"
                     /translation="MKCLSAWKFRLAVLGTVGILWSLSGCATGKWGFIDPSGKMAIQS
                     RFDDATPFAEGLAAVKIGEKWGYIDKTGKIVIEPQYDAAWRFRDGLASVMSGNKWGFI
                     DPKGKFVIQPQFESAGSFSEGLAPAETGGKWGFIDKSGKFSIQPQFGYAERFSEGLAE
                     VKVGDKWGFVDKTGQVAVQPEYVLVFPFSEGLAAVLAGKKFGFIDKTGKMVIKAQFDN
                     ALSFGDGLAPVKDGLQWGYIDKTGQIAIKPQFEVAFPFHDGVAPVKFEYKWGYIFNTG
                     QFAVWPKFDGAYAFNEGLGMVKLGRATGYIDKTGRYLRVF"
     gene            complement(5570..>5928)
                     /locus_tag="VEL68_01235"
     CDS             complement(5570..>5928)
                     /locus_tag="VEL68_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34620.1"
                     /translation="FRFQYSYVKSKGFADYTYLRTFVPADLGRNQDNIDINNLDNYTL
                     RYFLTRVTYNPVKSFSFSAGWVYEKYDYDDAQMNGYKYLATSSTGTTLSYLSGAYANP
                     SYKANVFFLSASYLFF"
BASE COUNT         1312 a         1677 c         1590 g         1349 t
ORIGIN      
        1 tctcccggga ccaagtggtt ctatatttac caggaggggt ataaggccct tttcatggga
       61 aaataatgag atgaacttga aaaaagtcgt cactccggtg aaaaccggag tccagagact
      121 tgatcacccc tttgaacaac tagattccgg ctttcgccgg aatgacgggg gaaagggggt
      181 tctgaccttt tcgaggccat caatggagcc ctctgggaca agcatgattc ccccagaggg
      241 ccttggctgg tttatggagt aaaggcctgg gcgacttttt tccccagggc atgacaggct
      301 tccaacccct tcttgtcggg gacatattga atgttcaacc ccgggtcgac gattcggaac
      361 ttcatcgcct ccaggtcctg gttgatcagc ttcaccgcct ccccgctcca gccgtaggag
      421 ccgaaagcgg cggcgatctt gttcagcggc ttgagccctt tcatgtagca caggaagtcg
      481 gccacggtgg gaaaaaggcc gttgttcagg gtgggggacc ccacgagaat ggccccggca
      541 tccaaaacct cggtcatcac gtcactccgg tggatggtcc tcaggtgcat cgtgcgggcg
      601 tccactctct cctgagcgag ggcatccacg atggcctcgg ccatggcctg ggtgctgtgc
      661 cacatgctgt cgtagatcac cagggccttt ttctggggct tctgctgcgc ccattggacg
      721 taggcccgaa tgatcttccc cgggtctttc ctccacatga ttccgtgatc cgggcagatc
      781 atcttgatcg gcaatcccat ccgggtgacc ttttccacca gctttaatat cagcggggag
      841 taaaggagga ggatgttggc aaaatacttt tgagcgtggg gcatgatcgc ctccccgatc
      901 tggtcgtcga acctttccat ccccgcatag tgctggccga aggcatcgct tgaaaaaaga
      961 atctggtctt ccttaaggta ggtgaacatg ctgtccggcc agtggagcat gcgagtttcc
     1021 aggaaggtga caattctctg gccaagtttc aattcttccc cctcattcac cggcttgagg
     1081 ttcaggcctc catggaaatg gcgggcgagg ttctgggccc ccaatttgga gcagtacacc
     1141 ggcttttcgc ttccgatcac gtgcatcaac cagtccaggc tgccggaatg atccagttcc
     1201 gcgtggttgc tgattacata gtcgatcttc ttcgggtcga tgaggaggcg gatctgatcc
     1261 atccactcgg gggaaaagtc cttcttcacc gtgtcgatga gaacgacttt ttcctccttg
     1321 atcaaatagg cgttataagt ggtaccctgg taggttgaat acccatgaaa gtcacgaatg
     1381 ttccagtcga tggaccctac ccaataaatg ttcttggcga tctctaaagg tttcatggct
     1441 ctctccttat tttaatgttg aatcctatcg tacgcagatg aatgcagatc aacgcaggtc
     1501 catttcgaag aaaagcagac cgaaaaccag gatttcagga acagcccact ccctctgcgg
     1561 gccgatcagt catcgataac tctcaagaat tttcgccagc aatgccccgt ccacgccttt
     1621 tcccaccaac ggcccctctt taagggccgg gaagtgagat tcaaaggcgg gccggttatt
     1681 ccagtagagg attcccaccg gaatgttttc cccccactcc tcggccgttt tcatggctac
     1741 ctcccagttg gtggggtcgt aatcgggggg gagttctttg cagcgggatt tataccagct
     1801 gaaggtgttc atcttgttga acgaaacgca gggctgaaga atatccacca gggaaaatcc
     1861 ccggtgggcg atggcttcct ggatgattaa ggccaggtgg tcaaccattc ccgcaaaacc
     1921 cctggccaca aacccggccc gtagggcaat ggcaacggcc accggattga agggggagga
     1981 agcgacacct tccggttgga ccttggtgac aaacccggtc tcggaagtgg ggctggcctg
     2041 gcctttggtg agtccgtaaa cctggttgtt atgcaccagg agagtgacat cgatgttccg
     2101 ccgcagggct gcgaggaaat ggtttcctcc ctccccgtag ttacagccgt ccccgctctc
     2161 cacaatcacc gttttcccgg gattggccaa tttcgcgccc gtggcaacgg gcagagaacg
     2221 gccgtggagg ccgtcgaata gatttacgtt cagatagtgg ggcgccttcg ccgcctgtcc
     2281 gatcccggag acgaagatga catcctgggg cctcagccgg caggcaacca acgcctgttt
     2341 caccgctttc aataccgaaa aattaccgca ccccggacac caggtcgtct cgtaatcccc
     2401 gtaatccttc aaggtcacca tggattgtct cccattctag tcctttatga attaacccaa
     2461 aatggtcaca acttgtaggg gcaaccctcc gtggttgccc atcttgcggg cggccacagg
     2521 aggccgcccc tacaataacc tatttggttc tggctctttc aggttaggta ggggcaattc
     2581 atgaattgcc cttacgatga tttgctcgtc ctccctgcgt aaggacggtt cgtgaaccgc
     2641 ccctgcgccc gggtcaattc aattcccgga gaatatattc cggggtgatg ggcagaccgt
     2701 cgtatcggcc gaccttcttt ttaatttcaa aaccggactc ctgccggatc agcttggcca
     2761 gctgccctgt ggcgtttcct tccacggaga tgacctggcg ggcgccttgg aggggaggca
     2821 ggaattgttc ctgaaccagg ggccaaacct gagggaaaaa aagggtggcc actttctttc
     2881 ccttggagcg cagatgagaa gctacttcca gaaccgatcc cttggtggac ccccaggtga
     2941 ccagaaggat ttccggattc ccatctccct ttaattccgg gggaatgacc tcctgccgga
     3001 ttccttccat tttcctcagc cttttatcca ccatctgcct gcggaccgaa aggtcttcgg
     3061 tgaggtgccc atcttccgtg tgctcgtcgc ttccggccac cacccggtgc tcgctccagc
     3121 ccggaaggag gcggggagaa atcccgtttt cggtgatcgc aaaccggcga tagggaaggg
     3181 atgcgggaac cgcgttccaa gagggtcccg caaggggaag cttttctacc tcaaaaggct
     3241 ctatggaacg gtaagaatcg gacaaaaatt ggtcagtgag gacaaagacc ggcccctggt
     3301 atttttctgc caaggcaaag gcccgacgag tcagatggaa acactcctcc acggttccag
     3361 gggcgaagat ggcccgggga aattcaccat gcccggcaaa gaggacaaat tgcagatcgg
     3421 cttgctccgt ccgcgtaggc aatcccgtgg ccggccccgg acgctgggcc accactatta
     3481 cgacgggggt ttcagtcatc cctgccaaac tgactccttc taccatgagg gcgaacccgc
     3541 cccccgaagt ccccaccatg ctcggagccc cggcataaga agcccccagg gccatgttga
     3601 tggccgcgat ttcatcttcg gcctgctcca ccaccagccc cattttcccg gcccacttgg
     3661 cgagggtttg accgatcgag gtggacgggg tcatggggta gaaggaataa aatttcagcc
     3721 cggcggagat ggcccccaga gcgatggcct cgttgccctg catcatcatc cgccgggttg
     3781 gattcgggac tggggagagt tttcccatga atgggggctg ggagagggtc caggccatgg
     3841 cgtcccgaag aaccttgcgg ttctcttcgg caacccccgg agccttcttg ccgaaaaaat
     3901 cctccagggt gcggccgacg acctcctcct tcaaacccat gaggccagag acgactccca
     3961 gagcggcgat attggaaaat tgatccgagg ccagatggcc gaaggggacc ttcagacaga
     4021 ctttttcatc cgcactgcac ccctgatccg cgacaattct cccccgggga gtcaattcct
     4081 tatcgtggag ggtgattgtc aaatcatcca gggccacgag gaggtcgacc tcctcccggg
     4141 aagcaatgac ttcatccggg ctcacccgga tggaaaaggt attgtggccg ccgcggattc
     4201 tggacatgta gctctgggtg accaggatat aatacccggc acgcaccagg ctcttggcca
     4261 ggatctctcc cacggtcacc aagccctgac cggcctctcc tccgattaga atattcaggg
     4321 tattcacgat tcttcctcct ttaggaaggc cctaccggtt ccagccgatt cagcaggatt
     4381 agaactatat tattacccag aaagcccttt tgagggaacg gggaaaatat cccattttgg
     4441 cagggggtat atagggggaa tacccgggaa caaaaaggag gggcttggaa ttaccttccg
     4501 gtcaatccta acccctccat tccatgaacg gtaataccgc ttccgagcag gatgtggcta
     4561 gattgggaaa agaagaacaa tcaaaagacc cgcaagtacc tcccggtctt gtcgatgtaa
     4621 ccggttgccc gccccagctt caccatgccc agaccttcgt tgaaagcata ggccccatcg
     4681 aacttgggcc agacggcgaa ctgtccggta ttgaagatgt aaccccactt gtattcaaat
     4741 ttcaccgggg caaccccgtc gtgaaaagga aaggccactt caaattgagg tttgatggcg
     4801 atttgccctg tcttatcgat gtagccccac tggagtccgt ccttcacggg tgccaaaccg
     4861 tcaccaaacg acagggcatt gtcaaattgc gctttgatga ccatcttgcc agtcttgtca
     4921 ataaacccga atttcttgcc ggctaacact gcagccaggc cctcggaaaa gggaaagacc
     4981 aagacatact cgggctggac ggctacttgt ccagttttgt cgacaaatcc ccacttgtcg
     5041 cccactttaa cttcggccag tccttcggaa aaacgctcgg cgtatccaaa ttggggctgg
     5101 atggaaaact ttccggattt gtcgatgaac ccccacttgc cccctgtctc tgctggggcc
     5161 agtccttcgg agaaagaacc ggctgattcg aattgaggtt ggatgacaaa tttccccttc
     5221 gggtcaataa agccccattt atttccggac atcacggagg ccagtccgtc gcgaaagcgc
     5281 caggcggcgt catattgcgg ttcgatgacg atctttcccg tcttgtcgat atacccccac
     5341 ttttcaccga tcttcaccgc ggccaagcct tcggcaaagg gggtggcgtc gtcaaatcga
     5401 gactggatgg ccattttccc gcttgggtcg atgaagcccc attttcctgt ggcgcaaccg
     5461 ctgagggacc aaagaatccc gaccgtcccc aatactgcta atctaaattt ccatgcgctc
     5521 aggcatttca ttcgtttcct ccttttgctc aacaccgcag agagcccggt taaaagaaga
     5581 gataggacgc gctcaggaaa aaaacattgg ccttatagct tgggttggcg taagccccgc
     5641 tgagatagct cagagtggtt ccggtcgaag aagtggcaag gtacttgtat ccgttcatct
     5701 gggcgtcatc ataatcatat ttctcataga cccagccggc ggagaaagag aaggatttaa
     5761 cgggattata ggtcaccctg gtcaggaaat acctcagggt gtagttgtcc aggttgttga
     5821 tgtcgatgtt atcctgattc cgtccaaggt ccgccgggac aaaggtcctc aggtaagtat
     5881 agtcggcgaa ccctttcgat tttacatagc tgtattggaa tctgaaaa
//
LOCUS       DASPRR010000033        16268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_167712, whole genome shotgun sequence.
ACCESSION   DASPRR010000033 DASPRR010000000
VERSION     DASPRR010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16268)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16268
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167712"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VEL68_01240"
     CDS             complement(<1..398)
                     /locus_tag="VEL68_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HXZ34621.1"
                     /translation="MAGIVSFGAYVPRYRLKRMTIFGAMGWINPANILLAQGEKAVAN
                     QDEDSLTMAAEAGIDCLKGFDRSKVDALYFASTTMPYVERQNSGIISMALNLRDDVRS
                     ADFSGALKSGTSALLSALEGVGSKSFRQAVV"
     gene            complement(431..658)
                     /locus_tag="VEL68_01245"
     CDS             complement(431..658)
                     /locus_tag="VEL68_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34622.1"
                     /translation="MLYLVGMAGIEASCCGTGGAAFIKVPGYIRAWKKGRNEAGLPVS
                     EVERIEAYGQRKEIQGLLQERHPGFTQVEFL"
     gene            complement(902..1150)
                     /locus_tag="VEL68_01250"
     CDS             complement(902..1150)
                     /locus_tag="VEL68_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34623.1"
                     /translation="MDVYPFHHRNLLFNVVTDLDLTFKEIREILDYLLEAKAFPKEKE
                     DNSEGGRIYDIRLGPFDYVVDVLGYEIVIYQRIENRGP"
     gene            complement(1486..2466)
                     /locus_tag="VEL68_01255"
     CDS             complement(1486..2466)
                     /locus_tag="VEL68_01255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HXZ34624.1"
                     /translation="MTKPKVLVTREVYERPLRLLQEKAEVTLNRENRSMTAEEIMAAL
                     PGKMGVLAMGGDPMNAKILEAGKELKIVANNAVGFNNIDLDAATRLKIAATNTPDVLT
                     DTTADLTFALILGVARRIAEADRFVRAGKWTGWTPSQMIGGDVHDKTLGVIGLGRIGS
                     AVARRGQGFNMRIVCYDIRRLDPAIEQQHQLQFFPLRDLLQRSDFVTLHVPLTPETKH
                     LIGAEELNLMKKTAYLINASRGPVVDEKALVEALRSGKIAGAGLDVFEAEPKVTPELL
                     KMENTVLVPHIGSATDETREAMTMRAVNNILAVIRGEVPPNILNPEIYQK"
     gene            complement(2579..3949)
                     /locus_tag="VEL68_01260"
     CDS             complement(2579..3949)
                     /locus_tag="VEL68_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ34625.1"
                     /translation="MAKQILVVDDEERIRQSLNGILKDEGYEVVESKDGVQALKQLEN
                     DPPDLVLLDIWMPGMDGMEVLERMKGQIPNLPVIMISGHGNIELAVKAVKLGAYDFIE
                     KPLSLEKVLLAVNNALLFSKLEQENRALRQEVERKYEIVGNSAEVQKLKEQIKIVAPT
                     NGWVLINGENGTGKELVARAIHRLSLRTGKSFVEVNCAAIPEELIESELFGHEKGSFT
                     GALTKKRGKFDLAHEGTLFLDEIADMSLKTQAKILRILQEQKFERVGGTEMIYVDVRV
                     IAATNRDLKEEIQKGKFREDLYYRLNVIPIGVPPLRERKTDIPLLVEHFIAEFCLENN
                     KEPKKASPEAVELLASYSWPGNVRELKNIVERMVIMTRGSVIEPKDIPDPVREQPKAP
                     LEFSFFDFDLLRDARREFEKRFIMKKLSENDENISKTAEMIGIERSNLHRKIKSYEIA
                     MRKEGP"
     gene            complement(4005..6245)
                     /locus_tag="VEL68_01265"
     CDS             complement(4005..6245)
                     /locus_tag="VEL68_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ34626.1"
                     /translation="MTEEPKKNKAGKRRRRELILIFVLLVWIGLLFFFQTHVSQLGDK
                     IPVTSNILVISLIGINLLLFLLLVFLILRNIVKLVLERRKKVLGAKLKTKLVVAFISL
                     TLVPTIVLFITAIYFITSSIENWFSIQVEGSLQESLVVAQVYYQDQASSALHHGRQMS
                     QAITQQKMLDRGQQELLAGYLKAKQGEYNLAAIEIIIPHLTSSLFIRNPNLASISFPP
                     LESKLLQEAFRGKEATKVQSIGQGDLIHGLVPVFSPQNPREVGGVLVVVYFVPQSLVS
                     RVTQISTAFEDYKQLSALKNPIKFSYLVTLSLVTLLIIFSATSFGLFLAKGITEPVQK
                     LVEGTQKIVKGNLDFAIPKTSGDEVGTLVDSFNQMTQDLKFSKSQVEKAHADLSKSNL
                     ELDQRRRYMEVVLRDVGTGVISLDSAGRIRTINRSAERILGIHAGQVLGKEYTELLQG
                     ESLQLAHDLVRAVNRAENHSITRQIKLRVRNDLLTLLVKVAMLRDEEERYLGMVVVLD
                     DLTQLQKAQRAEAWREVARRIAHEIKNPLTPIQLSAQRLRKRYLNQFGQDGAIFDECT
                     QTIVTQVEELKNLVNEFSSFARMPAAQLMPNNLNEIIREAMVLYVEGHKNIHFEFKPD
                     EKVPVFNLDKEQIKRVIVNLLDNAVAAVHGVAGKDGEGQITIDTQLDPLMNLARIEVA
                     DNGCGVSPEDKPLLFEPYFSTKPSGTGLGLAIVNTIVSDHHGYARVRDNVPRGTRLII
                     ELPINQ"
     gene            complement(6469..7371)
                     /gene="lpxC"
                     /locus_tag="VEL68_01270"
     CDS             complement(6469..7371)
                     /gene="lpxC"
                     /locus_tag="VEL68_01270"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HXZ34627.1"
                     /translation="MISQITVRNPIMCKGVGLHTGLPAHMVIHPAPTDTGIVFIRKDV
                     GNRVRIPARIDNVVDATLATTISFDGVKVSTVEHLMAAFAGLGIDNAEVELDAPEVPI
                     MDGSSDPFNTLLKKAGVRTQDRPKKFVIIRHPVTVTDGDRQATLLPSNDFKLSYTIDF
                     RHPLISNQFYLIQISNGNFEREICRARTFGFLREYEILKARGFARGGSLENAVVVDDS
                     RVLNEGGLRFSDEFVRHKILDSIGDLWLLGAQVIGHYIGYKSGHTLNHKLIHKLFAHR
                     EWWDLKEFSNEEEVRKLQITPPTH"
     gene            complement(7676..8191)
                     /locus_tag="VEL68_01275"
     CDS             complement(7676..8191)
                     /locus_tag="VEL68_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXZ34628.1"
                     /translation="MAGRYGETLGQYLRRERESRSISLKELSKNTRISVAFLEAIEKD
                     DFEAFSRQEFILGFLQGYARQLGLKGEEVLRRYRIQAELAGRKEKFQQMPLFPGSGAS
                     AEEEEETMPEWEEPLPSSSKKRIPWRMLVQMAIVLFALGLSFYLHQKLKNSDPREQIS
                     NPGGIPPEKGR"
     gene            8352..9914
                     /locus_tag="VEL68_01280"
     CDS             8352..9914
                     /locus_tag="VEL68_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXZ34629.1"
                     /translation="MAMNPVMEKLLKELDERKRKIELAGGADKIEKQHQAGKLTARER
                     ILKLLDPDSFMETDMFVHHRCEYFGMSKVEAPDDGVVTGYGTMNGRLIFIFSQDFTVL
                     GGSLGDAHASKIVKIMDLAAKVGAPLIGINDSGGGRIQEGIDALKGYSQLFYRNSIYS
                     GVMPQISAILGPCAGGAVYSPAITDFIFMTEGISRMFLTGPNVIKEVTGEEISDEELG
                     GAKVHNWISGVAHFYSQTEEECFQQIRRLMTFLPSSNRENPPIRYSGDPLDRRNEKLF
                     QVVPVDGKRPYNVIKVIEEIVDDHDFFEVHQYWARNMVVGFARIHGHSIGVIANQPMV
                     LAGTLDINASDKAARFVRFCDCFNIPLLTLVDTPAFLPGKAAEFAGIIRHGAKILFAY
                     SEATVPKITLVLRKGYGGGYVAMCHRELGADAVFAWPMAEIAMMGAEGAANIVSRKEI
                     EKAPDPEKKRREKIQEYRDQFSNPYASGRRGYVDYIIRPDETRSMICRAFEMLWTKQE
                     ERPKKKHGSIPL"
     gene            complement(9992..10453)
                     /locus_tag="VEL68_01285"
     CDS             complement(9992..10453)
                     /locus_tag="VEL68_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016749473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXZ34630.1"
                     /translation="MPKRMIQNLEELKKMMGQEVGVSDWHPVTQQAINLFADATLDHQ
                     WIHIDVERAKRESPFGGPIAHGYYTLSLAPYLLANVFDVKEKKMGVNYGLNKLRFTAP
                     VPIPSRVRVRATLAQIEEIKGGVQATFNLQFEVEGKDKPACVAEAIYRYYA"
     gene            complement(10519..11502)
                     /locus_tag="VEL68_01290"
     CDS             complement(10519..11502)
                     /locus_tag="VEL68_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXZ34631.1"
                     /translation="MDQGRKKVIVSTNSFYAAKVKLLEETGFEVQFLPDRADNLFLKE
                     IGKAHALIPGLSPVTAQVMEKAPGLLIVAAHGVGYNNIDLQAADQMGILVTNIPGVNA
                     DAVAEFTFGLILALIRRIPQAWEEMRRGGWRRPELWGSELRGKTLSIIGLGRIGSRVS
                     RVGVAFGMKVLACDPYIPEQAFREAGAKPVSRNEAISQADFLTLHIPLTEETRMMIGS
                     RELSLMKKSALLINTARGGILDDRELVKALDDGVLAGAGIDVFEEEPPDERSLRSHPK
                     ILSTPHIAGLSDEARYRMSRGASERVACALRGERPPDVVNEPRNPRYLRMA"
     gene            complement(11602..12600)
                     /locus_tag="VEL68_01295"
     CDS             complement(11602..12600)
                     /locus_tag="VEL68_01295"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXZ34632.1"
                     /translation="MNREKPIVGITMGDPAGIGPEITVKAIVSPEIQNVCRPMIIGDA
                     RVARKALREENIDVPIHVVTSLGEANWNSSGIRILDTRNADPEKIAVGQPSAVGGRAV
                     FEDVERAVQLAQGKQVSGIVAGPHNKYSVNLAGVPFAGYPPLIAKLTGSPYSFNMLVS
                     GTIRIVGATLHLALKDVCGAVSQDLVLAVIEAVQTALNRLGIPHPRIAVAGLNPHCGE
                     EGLFGTEEETIIGPAIETAQRQGIKASGPYPADSLFVRLKESPAFDAYVAMYHDQSHI
                     PIKTVAFDSIAGLIIGTPLVIATVGHGTAFEIAGKGRANPRPMKEAITLVALSCDA"
     gene            complement(12680..14191)
                     /locus_tag="VEL68_01300"
     CDS             complement(12680..14191)
                     /locus_tag="VEL68_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ34633.1"
                     /translation="MEQLTGLYYGFSIALTWQNLTYCLLGTLVGTLVGVLPGLGPAAT
                     VALLLPITFKLPPVSAIIMLSGIFYGAMYGGSTTSILLNIPGEAASVVTCIDGYQMAL
                     QGRAGPALAIAAIGSFIAGTIGIVGLNFLAPPLAEFAVQFGPPEYFALTLFGLFLAAY
                     LASSSMVKGLIMVVLGLMLDEVGIDPIMGVARFSYGVLPLQEGLDFVPLAMGLFGIAE
                     ILINTEKAMKTQLVTKKIDRIFISLRDWAEAKWATLRGSVLGFFVGILPGGGAVIASL
                     ISYAVEKRVSRNPEKFGKGAIEGVAGPEAANNSAATGSFIPLLTLGIPGNATIAMIFA
                     ALLIHGVQPGPYLIKENPEIFWGVIASMYIGNGFLLILNLPLVGMWVQVLRVPYFILA
                     PFILLLCIVGVYSTRNNIFDIWVMIFFGVVGYFFRKLEFEPGPLILAFVLGAISERTF
                     RQSLLMSKGSPWVFFARPISCALMVMLLALVALQVFFMIKRKTKSLDQFRGAG"
     gene            complement(14211..14660)
                     /locus_tag="VEL68_01305"
     CDS             complement(14211..14660)
                     /locus_tag="VEL68_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ34634.1"
                     /translation="MNAGSILAGIIFLALGIFVCQQAIQLSLGQASRPGPGFVPFGLG
                     SVLILLSLLYLVPGFRAFKQEEDLHPSPGLIRTLLAIGILCLFSAILSWLGYLVSTLL
                     LFLLWLMLMERKKWPMAVGLAGLALVFVYFFNLLFSIQLPRGIIRGL"
     gene            complement(14666..15652)
                     /locus_tag="VEL68_01310"
     CDS             complement(14666..15652)
                     /locus_tag="VEL68_01310"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34635.1"
                     /translation="MKKGFPIFAAGWIAVAACLFIPLQGTAQDKDYPNQPITLIVPWG
                     AGGASDQAARAVARYAPTYVGQQMIVVNKPGAGGAVGMETVIRSKPDGYTLLSASIGS
                     NTLTPAFQAGLPFQFDEVTFICRTQLNPAVLVVKTEAPWKTLKDLVDDVKKNPGKFLY
                     GTSGIGSMHNWATYKFLKAAGIDMQSVTMIPFNSGKEQVVAMLGGNIHFSYMNLSEPY
                     AQLKAGKLRALALAPKRMEEVPNVPTFAELGYPQVDVMGWRGVSGPPNLPEGIVKKLE
                     SGLEKLCRDPGWIDLVIKMGDLPAYIGPKDFKAFVTKEYNEAKNDAKSLGIK"
     gene            complement(15762..>16268)
                     /locus_tag="VEL68_01315"
     CDS             complement(15762..>16268)
                     /locus_tag="VEL68_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HXZ34636.1"
                     /translation="IAEAFKPQATVDSIIEAGKAYVNENIGQIIDECIGYVKQVPDML
                     EVRELYWKRFGGRVPTDSLEVVGESFAMFWLTKGDPKQCMIAGSSLGRDTDCIASIAG
                     AIAGAFKGIDAIPKEWVDICQKAMSADPHEIITMSMEDQSKALYDMVLKIMGERKKQV
                     EIIESLKK"
BASE COUNT         3531 a         4640 c         4348 g         3749 t
ORIGIN      
        1 accacggcct ggcgaaagct cttggaccct actccctcca aggccgaaag aagagccgat
       61 gttcccgatt tgagagcccc tgaaaaatcg gcagagcgaa catcgtcccg caggttcagg
      121 gccatggaaa taatgcccga attctgcctt tccacgtagg gcatagtggt cgaggcaaaa
      181 tagagggcat ccaccttgga ccggtcgaaa cctttcaggc agtcgatacc ggcttctgcg
      241 gccatggtca ggctgtcctc atcctggttg gccactgcct tttccccttg ggccagcaga
      301 atgttggcgg ggttaatcca gcccatggct ccgaagatgg tcatccgttt tagccggtaa
      361 cgggggacat aggcgccgaa ggaaacaatt ccagccatat tcacctcctc gatgaaatgt
      421 aggaacaaat ctacaggaac tcaacctggg taaaacccgg atgtctttcc tgcaaaagac
      481 cttgaatctc tttccgctga ccgtaggctt ctatcctctc cacttccgag accgggagcc
      541 cggcttcgtt ccgccccttc ttccaggccc ggatgtagcc gggaaccttg atgaaggcgg
      601 cccccccggt tccgcaacag gaggcttcga tgccggccat tcctacgaga tagagcacct
      661 ccttcccccg ataatctaac ttcccttcct ctacaaacag gtatcccccg ccgaaaaaat
      721 caactctttg gtttacttct ggatggacga attcaaaaac atgctccgaa atggtaagcc
      781 cctccgggaa tgagctctgg tttcccttat tctcgtggat tcaatccttt ttcctctgtc
      841 cccttccctt tcttttatga gcccatttcg cgggagaaaa ggaagaatcc agagaatcga
      901 tttatggacc tcgattctcg attcgctggt agatgacgat ctcataaccc aatacgtcta
      961 ccacataatc gaatggaccc aggcgaatgt cgtagatccg accgccttcc gagttatctt
     1021 ccttttcttt aggaaaggct ttggcttcca gcaggtaatc cagaatctcg cggatttctt
     1081 tgaacgtcag gtccagatcg gtgaccacgt taaataaaag atttcggtga tggaacgggt
     1141 aaacatccat ggtttgcccc gggaaaaatt tggccccatc ctaacaaaaa acaaaacctt
     1201 catcaagatt tttttcgccc ccgaataata cgccctcaaa ctaatttcct ggagctacac
     1261 aggggagtct aagaatacac tggcaggcgg catttttttg gccacctgtg gagcgtgtcc
     1321 ttttttcaaa atgagttgca aaccttgagg aagaaggatt tttgggggcc ctgcgccggg
     1381 aaggaaactt acgggataaa caagagcccg ccccaattga gccgtcttct tggacccaga
     1441 gccggaacgc agaaccttcc ggctcctgaa tccctgcccc cgattctatt tctgataaat
     1501 ctccgggtta agaatattgg gtgggacctc gccccggatg acggccagga tgttattcac
     1561 cgctcgcatc gtcatcgctt cccgggtttc atcggtggcg ctgccgatgt ggggaacgag
     1621 aacggtgttt tccatcttaa gaagctcggg cgtgaccttt ggctcggcct cgaaaacgtc
     1681 cagaccggcc ccggcaattt ttcccgaacg cagggcctcg acaagggcct tctcatccac
     1741 caccggcccc cgggaggcat tgatcaggta ggcagtcttc ttcatcagat tcaactcctc
     1801 cgcgccgatg aggtgtttcg tttcgggggt caggggaaca tgaagggtga cgaaatcgga
     1861 cctctgaaga aggtccctca gggggaagaa ctggagttga tgctgctgct caatggccgg
     1921 gtccaggcgg cggatgtcat aacagacgat ccgcatgtta aacccctgcc ctcgtctggc
     1981 cacggccgag ccgattcttc ccaggccgat gactcccagg gttttgtcat gaacgtcccc
     2041 accgatcatt tggctggggg tccagcccgt ccatttcccc gcccggacaa accggtctgc
     2101 ctcggcgatc cgacgggcca ctcccagaat caaggcgaag gtaaggtcgg cagtggtatc
     2161 ggtcaggaca tccggggtgt tggtggcggc aatcttcagc cgcgtagccg catccaggtc
     2221 gatgttgttg aaccccacgg cattattggc aacgatcttc aattccttgc ctgcctccag
     2281 gattttggcg ttcatggggt ccccccccat ggccaggact cccattttgc cgggcagggc
     2341 ggccatgatc tcctccgcgg tcatggaccg gttttcccgg ttcaaggtca cctcggcctt
     2401 ctcctgcaat agccgcaagg gcctctcgta aacctctcgc gtcaccagaa ctttaggctt
     2461 cgtcattcct catttccttt ccttttttat tcgccctctc tcattggccg aacgggtcca
     2521 ccggagagga acgaagtcat tgcccggagt cagcctcctc cggatccccc gttttctttt
     2581 aaggaccctc ttttctcata gcgatttcgt agcttttgat ctttcggtga aggttgcttc
     2641 tttcgatgcc gatcatctct gcagtcttgg agatgttctc gtcgttctcc gagagttttt
     2701 tcatgatgaa ccgcttttca aactcccgcc gagcgtcgcg cagtagatca aaatcgaaga
     2761 aagagaattc caggggggcc ttgggctgct cccggacggg atcggggatg tctttgggtt
     2821 cgatgaccga cccccgggtc attatgacca tccgctccac gatgttcttc agttctcgca
     2881 cgttgcccgg ccaggaatag gaggccagca gctcgacggc ttccggggag gcctttttag
     2941 gctctttgtt gttctcgagg caaaactcgg cgatgaaatg ctccaccaga aggggaatat
     3001 cggtcttccg ttctcgcagg ggtggaaccc cgatggggat tacgttcagt cggtagtaca
     3061 agtcttctcg gaatttgccc ttctggatct cttccttcag gtcccggttg gtggcggcga
     3121 taacccggac atccacgtag atcatctccg ttcctcccac ccgttcgaac ttctgttcct
     3181 gcaggatgcg cagaatcttg gcttgggttt tcaaactcat gtcggcgatt tcatccagga
     3241 aaagggtccc ctcgtgggcc aggtcaaact tgcctctctt cttggttaat gccccggtga
     3301 aagagccttt ctcgtgtccg aaaagttcgc tctcgatgag ctcttcggga atggctgcac
     3361 agttgacctc gacaaatgat tttcccgtcc gcaggctcaa ccggtgaatt gcccgggcaa
     3421 ccagttcctt cccagttcca ttctccccgt tgatcaggac ccagccattg gtaggggcga
     3481 cgattttgat ctgttccttc agtttctgga cctccgctga gttgcccacg atttcgtatt
     3541 tcctctccac ttcctgacgg agcgcccgat tctcctgttc cagcttggag aagagcaagg
     3601 cgttgttcac cgccaggaga accttctcca gggagagggg cttttcaatg aaatcatagg
     3661 cgcccagttt aaccgctttc accgccagtt cgatgtttcc gtgcccggag atcataataa
     3721 ccggcaggtt gggaatctgc cccttcattc tctccaggac ttccatgccg tccattcccg
     3781 gcatccaaat atccaggagg acgaggtccg ggggatcatt ttcgagctgc tttaaagcct
     3841 ggaccccatc tttggactcc acgacttcat agccttcgtc cttgaggatc ccgttcaggg
     3901 actgacggat tcgctcttcg tcatcgacca ctagaatctg cttggccatg ggtttccttc
     3961 ggcagacggg taagtttcac gattgcagga tcgggaatcg tctttcattg attaatggga
     4021 agctcgatga tcaagcgggt cccccgggga acgttgtccc tcacccgggc gtatccatgg
     4081 tgatccgaaa caatggtgtt gacgatagcc agccctaatc ccgttcccga cggcttggtg
     4141 gaaaagtagg gttcgaagag aaggggtttg tcttccgggg ataccccaca cccgttatcg
     4201 gccacttcga tccgcgccag attcatcagg ggatcgagct gggtgtcgat ggtgatttgc
     4261 ccctctccgt ccttgcctgc cacgccatgc accgccgcca ccgcattatc cagcaggttg
     4321 acgatgaccc ttttgatctg ctccttgtcc aggttgaaca ccggtacctt ctcgtctggc
     4381 ttgaactcga agtggatgtt tttatgtccc tccacataaa gcaccatggc ctcccggata
     4441 atttcgttga ggttattcgg catgagttgg gccgcgggca tgcgggcaaa gctggaaaat
     4501 tcatttacca ggttcttcaa ttcctccact tgggtgacga tggtttgggt gcattcgtcg
     4561 aaaatcgctc catcctggcc aaactggttc aggtaccgtt tccgaagccg ctgggccgaa
     4621 agctggatag gagtcagggg attcttgatc tcgtgggcga tccggcgggc cacttcccgc
     4681 caggcttccg cccgctgggc tttttgcagc tgggtgagat cgtccaggac caccaccatc
     4741 cccaggtagc gttcttcctc gtcccggagc atggccactt tcaccagcaa ggtcagcagg
     4801 tcgttccgca cccggagctt gatctgtcgg gtgatggagt gattctccgc tcgattcacc
     4861 gcccggacca gatcatgggc cagctggaga gattcacctt ggaggagctc ggtatactct
     4921 tttcccaaca cctggcccgc atgaattccc aggatccgtt ccgccgaccg gttgatagtc
     4981 cggattctgc ctgcggaatc gagggaaatg acccccgttc ccacatcgcg cagaactacc
     5041 tccatgtacc gtcgtctttg atcgagctcc aggttgcttt tgctcaggtc ggcatgggcc
     5101 ttctccacct ggctcttact aaacttcaag tcctgggtca tctggttgaa ggaatccacc
     5161 agggttccga cttcgtcgcc cgaggttttt ggaatggcga aatccaggtt tcccttgacg
     5221 atcttctggg ttccttccac cagtttttgg accggttcgg tgatcccctt ggctaaaaac
     5281 aggccgaagg aggtggccga gaaaatgatc aatagggtta ccagagaaag ggtgaccaga
     5341 tagctgaact tgatcggatt cttaagggcg ctcagctgct tgtagtcttc aaaggcggta
     5401 gagatctggg taacccggga aaccaggctc tggggaacga aatagaccac caccagaacc
     5461 ccaccgacct cccgcggatt ctggggagaa aaaacgggaa ccagcccgtg gatgaggtcc
     5521 ccctgcccta tggactggac cttggtcgcc tcttttcccc ggaaagcctc ctggagcaat
     5581 ttggattcga ggggagggaa ggagatcgaa gccaggttgg ggttgcggat gaaaagcgag
     5641 ctggtcaaat gggggatgat aatctcaatc gccgccaggt tatattcccc ctgtttggcc
     5701 ttcaggtacc cggcgagtag ctcctgttga ccccggtcga gcatcttttg ctgagtgatg
     5761 gcttggctca tctggcggcc atggtgaagg gcgctgctgg cctggtcctg ataatagact
     5821 tgggcgacaa cgagggattc ctgcagggat ccctccactt gaatactgaa ccagttttcg
     5881 atactgctgg tgatgaaata gatggcagtg atgaagagga cgatggtggg caccagggtc
     5941 agggaaataa aggccaccac cagtttggtt ttgagcttgg cccccaggac cttttttctt
     6001 ctctcgagga ccagcttgac gatgttacgc aggatgagga agacgagtaa aaggaacaga
     6061 agaaggttga ttccgatgag agagatgacc aaaatgttgc tggtcaccgg aatcttgtcc
     6121 cccagctgag agacgtgggt ttgaaagaaa aagagcagac caatccagac caggagaacg
     6181 aaaatcagaa tcagctcccg tcgcctccgc ttgcctgcct tattcttttt tggctcctcg
     6241 gtcatggttg ggttagggac ttcgccccgg cccttgccct tcggacgatt ccccgttctt
     6301 tattagtttc aatatcttac ccaatttccc cggaaaaagc aaggatcggg tgaggataga
     6361 acctgcgggc tctaaaaaag ccaaacccgg gccagggtct ttccccgccc aaagcccctc
     6421 cctttcggaa agaaaggacc tgaaagcctc ctgaatcgat gctaagaatc agtgggtggg
     6481 tggagtgatc tgaagctttc tcacttcttc ttcgtttgaa aactctttga ggtcccacca
     6541 ttcccggtga gcaaacagct tgtggatcag tttgtgattc aacgtgtggc ccgacttgta
     6601 cccgatgtaa tgcccgatga cttgagctcc cagcagccaa aggtccccga tggaatccag
     6661 gatcttatgc cgcacaaatt catcggaaaa gcgcaagccc ccctcgttga gcacccgaga
     6721 gtcgtccacc accacggcat tttccagcga ccctccgcgg gcgaatcctc tggccttcaa
     6781 aatttcgtac tcccgcagaa aaccgaaggt cctggcccgg cagatctccc tttcgaagtt
     6841 tccgttggaa atctggataa ggtagaactg gttggaaata aggggatggc ggaagtcgat
     6901 ggtataggag agcttgaaat cattgctcgg cagaagagtc gcctgccggt ctccatcggt
     6961 taccgtcacg ggatggcgga tgatgacgaa tttcttcggt ctgtcctgag tccggactcc
     7021 ggcttttttc aaaagagtat tgaaaggatc ggagctccca tccatgatgg gaacttcggg
     7081 agcatcgagc tccacttcgg cattatcgat tcccaagccg gcgaaggcgg ccatcaggtg
     7141 ttcgacggtt gaaaccttca ctccatcgaa actgatggtg gtggccaaag tggcatccac
     7201 tacgttatcg attcgggctg gaattctgac cctgttcccc acgtccttgc ggatgaaaac
     7261 aatccccgtg tcggtcggag caggatgaat gaccatgtga gctggcaagc ctgtatgcaa
     7321 cccaactccc ttgcacataa ttgggtttct aactgttatc tgagaaatca ttttgcacaa
     7381 actcctcgcc aaatttcata tttttagagt cttttagcaa gtttaatgcc ataatgacct
     7441 gccaaaactt attttttcat ccaaacccga aagatttgta agacattcga atgatggaga
     7501 atttatccat ctaaaagccc tcccggagtt atccaggccg gctggcatcc cctcccggtt
     7561 ttacaatgtg gcaaacctga cacaatcata ttcggggtta ttgttttttt gcaacctttt
     7621 tatttttccc ctggcctttt tccttctcgg aaaccccgtg agaagatcgg cccctttatc
     7681 tccctttttc cggcgggata cctcctggat tggaaatctg ttcccgcggg tcggagtttt
     7741 tcaacttttg gtgaaggtag aaagaaagcc ccagagcgaa aagaacaatg gccatttgga
     7801 ccagcattct ccagggaatt ctttttttcg aggaagacgg aagaggttcc tcccattcgg
     7861 gcattgtttc ctcttcttcc tccgccgaag ccccggaccc cgggaaaagc ggcatctgct
     7921 ggaacttctc ttttcgaccg gccagttcgg cttggattcg gtaacgccgc aagacctctt
     7981 cccccttcag gccgagctgg cgggcatacc cctgcaggaa cccaagaatg aattcctgcc
     8041 gggaaaaggc ctcgaaatcg tccttctcga tggcttccag aaaagccacg ctgatccggg
     8101 tgttcttgga caactccttc agcgaaatgc tccgggattc ccgttcccgc ctcaagtact
     8161 gccccagggt ttctccgtaa cggccagcca ttttcaaccg gatgaaagag gtttttgggg
     8221 gggttggatt cttttcattc cagctccctt ttatagcaca ggcttttggc cgtgccaaca
     8281 aaatcttgac aaaaagggat gatcttggta ttcatcctat tcaatttcaa ttgcatctgg
     8341 gaggaacccc gatggcgatg aaccctgtga tggagaaatt gctgaaggaa ctcgatgagc
     8401 gcaagaggaa aatcgagctg gccgggggag cggacaagat tgaaaagcag caccaggcgg
     8461 gaaagctgac tgccagagaa cgaatcctca agctcttgga cccggatagc ttcatggaaa
     8521 cagatatgtt cgtccatcac cgctgtgaat attttgggat gagcaaggta gaggcgccgg
     8581 atgacggagt agtaaccgga tacgggacca tgaacgggag gctgatcttc attttctccc
     8641 aggacttcac cgtcctgggg gggtcgctgg gagatgctca tgctagcaag atcgtgaaga
     8701 ttatggactt ggcggccaaa gtgggagccc ccctgatcgg aatcaatgat tcgggcggcg
     8761 gacgaatcca ggaggggatt gatgccctga aaggctactc tcaactcttc taccgcaact
     8821 caatctactc gggggttatg ccccaaattt ccgccatcct gggtccctgc gccgggggcg
     8881 cggtttactc tcccgccatc accgatttca ttttcatgac cgaggggatc agccgaatgt
     8941 tcttgaccgg gcccaacgtc atcaaggagg taaccggaga ggagatcagc gatgaagagc
     9001 tggggggggc caaggttcat aattggatca gcggggtggc ccatttttac tcccagacgg
     9061 aagaggaatg ctttcagcag attcgtcgcc tgatgacctt tttgccctcc agcaaccggg
     9121 aaaatccacc catccgctac tccggagacc ccctggaccg gaggaatgaa aagcttttcc
     9181 aggtcgttcc ggtggacggg aaacggcctt acaatgtgat caaggtcatt gaagaaatcg
     9241 tcgacgacca cgattttttc gaggttcacc agtactgggc ccgaaacatg gtggtgggat
     9301 tcgcccgcat ccacggccat tccatcgggg tgatcgccaa tcagccgatg gtgctggccg
     9361 gcactctgga tatcaacgct tcggacaaag ccgccaggtt cgtccgcttc tgtgactgct
     9421 tcaacattcc cctgctgacg ctcgtcgaca ccccggcctt cctgccaggg aaggccgcgg
     9481 agtttgccgg catcatccgc cacggggcca aaattctctt cgcctattcg gaggccacgg
     9541 ttcctaaaat caccctggtc ttgcgcaagg ggtatggagg gggatacgtg gccatgtgcc
     9601 accgggagct gggagcggat gcggtctttg cctggcccat ggccgagatt gccatgatgg
     9661 gggcggaagg ggcggccaac atcgtctctc ggaaagaaat cgagaaagcc cccgacccgg
     9721 agaagaaacg gcgggagaaa atccaggagt accgtgacca gttctccaac ccctatgcct
     9781 ccggacggag gggttatgtc gactacatta tccggcccga tgagactcgt tcgatgatct
     9841 gccgggcctt tgaaatgctc tggaccaaac aggaggaacg acccaagaag aagcacggat
     9901 cgattcccct ctaataacag catagtgcag agcgcatagt gcacagcaca actttctttc
     9961 ttcttcaacg ctatgctcta tgccctttgc gctatgcgta gtatctgtag atcgcctcgg
    10021 ccacgcaggc gggtttgtct tttccctcca cttcgaattg caggttgaaa gtcgcctgaa
    10081 cccccccttt gatctcttcg atttgagcca atgtggcccg caccctgact ctggatggaa
    10141 tgggcacggg agcggtgaag cgcagcttgt tcaacccgta gttgaccccc atcttcttct
    10201 ccttaacatc aaaaacattg gccagcagat aaggagccag gctcagagtg tagtacccat
    10261 gagcgatggg acctccgaaa ggcgattccc gcttggctcg ctccacgtcg atgtgaatcc
    10321 actggtggtc cagcgtggca tcggcgaaca ggttgatggc ctgctgggtc accggatgcc
    10381 agtcgctcac ccccacctcc tgtcccatca tcttcttcag ttcttccagg ttttgaatca
    10441 ttcttttggg cataaggacc tcctttcttt tcttccgtgg atgagacctt cttctcgggt
    10501 actttctctg aatttttctc aggccatccg caggtaccgt ggatttcggg gttcgttgac
    10561 cacgtccggg gggcgctcgc cccgaagggc gcaggccacc cgctcggaag ccccccggga
    10621 catgcggtac ctggcttcat cactcaggcc ggcaatgtgg ggagtggaga ggattttcgg
    10681 gtgcgacctc aggctccgtt cgtcgggagg ttcctcttca aagacatcga tccccgcccc
    10741 agccaggacc ccgtcgtcca gagctttgac taactcccga tcgtccagaa ttccccctcg
    10801 ggcggtattg atcaagaggg ccgatttttt catcagggaa agctcgcggc tcccgatcat
    10861 catccgggtc tcctcggtga ggggaatgtg gagggtcaaa aaatcagcct gggaaattgc
    10921 ttcattccgg gataccggtt tcgccccggc ttcccggaaa gcttgttccg gtatgtaagg
    10981 gtcgcaagcc aaaaccttca ttccgaaggc cactcccacc ctgcttaccc ggctcccaat
    11041 cctgccgagt ccgataatgg acaaggtttt tccgcgcagc tcagaacccc aaagctcggg
    11101 acgccgccac cctccccttc tcatctcctc ccaggcttgg ggaatgcggc ggatcagggc
    11161 caggatcagc ccaaaggtga attcggccac agcatcggca ttgacgccgg ggatgttggt
    11221 taccaggatt cccatttggt cggccgcctg aaggtcgatg ttgttgtacc ctaccccgtg
    11281 ggcagccacg atcagcaggc ctggagcctt ttccatgacc tgggctgtca ccgggctcaa
    11341 tcccgggatg agagcatggg cctttccgat ttccttaaga aacaggttat cggcgcgatc
    11401 cgggagaaat tggacctcga accccgtctc ttcaagaagc tttaccttcg ctgcataaaa
    11461 ggaattggtg ctgacaatga ctttttttct tccctgatcc atcagaatat ccctttccct
    11521 ccctctcccc tgcgcccttt ttcttcgcga tcgcgccacc cgcggcattt caacggagcc
    11581 cccgggggga aaaagcatgc cctacgcatc acaactcagg gcaactagcg tgatggcctc
    11641 cttcatgggt cggggattgg cccggccctt tccggcgatc tcaaaggcag taccgtgacc
    11701 caccgtggca atcaccaggg gtgtgccgat gatgaggccg gcgatcgaat cgaaggccac
    11761 cgtcttgatg gggatatgag attggtcgtg atacatggca acatatgcat cgaaggccgg
    11821 gctttccttg agccttacga aaaggctgtc cgccgggtag gggccggaag ccttgattcc
    11881 ttgcctttgg gcagtttcaa tggccgggcc gatgatcgtc tcctcttctg tcccgaaaag
    11941 cccctcctct ccacaatgcg gattcaaccc ggcgacggcg attcgaggat gagggatccc
    12001 cagcctgttc agggcggttt gcaccgcttc gatgacggcg agcactaagt cctggctgac
    12061 ggccccgcag acgtccttca gggccagatg cagcgtggcc cccacgatcc ggatcgttcc
    12121 cgacaccagc atattaaaag aatacgggct cccggtaagt ttggcgatga ggggtggata
    12181 tccggcaaaa gggaccccgg ccaggttcac ggaatatttg ttgtgggggc cggccacgat
    12241 cccggagacc tgcttcccct gggccagttg gaccgccctc tcaacatcct caaagaccgc
    12301 tcttccgccc accgccgacg gttgccccac agcaatcttc tccgggtccg cgtttcgagt
    12361 gtcgaggatg cgaatccccg aggaattcca gttggcttca ccaagcgagg taacaacatg
    12421 aatcggaaca tcgatatttt cctcccggag ggccttgcgg gccacccggg catcgccgat
    12481 gatcatcggc cggcatacat tttgaatttc aggggagacg atagctttca ccgtgatttc
    12541 cggcccgatc cccgctggat cccccatggt tatccccacg atcggttttt cccgattcaa
    12601 atttccactc ctttaatctt ttgaccgcct tactcggcgt tattcattcc gcattccaca
    12661 ttccgaaacc cgcattcgat taccccgcac cccgaaactg atccaggctc ttagtctttc
    12721 tttttatcat gaaaaagacc tgaagagcca ccagagccag gagcatgacc atgagagcgc
    12781 aggaaatggg ccgggcgaaa aagacccagg ggctcccctt ggacataagc agagactgcc
    12841 ggaaggttct ctcggaaatg gctcccagga caaaggccag gatgagcggg ccaggttcga
    12901 actccagctt gcgaaagaaa tatccaacca ccccgaagaa gatcatgacc cagatatcga
    12961 agatgttgtt gcgggtgcta tataccccca cgatgcacag gagaaggata aaaggggcta
    13021 ggatgaaata gggaacccgc agaacttgca cccacattcc caccagggga aggttcagta
    13081 tgaggaggaa gccattgcca atgtacatgg aggcgatcac tccccaaaaa atctccgggt
    13141 tctctttgat cagataaggg cccggctgca cgccgtgaat cagaagagcg gcaaagatca
    13201 tggcgatggt ggcatttccc ggaatcccca gggtcagcag aggaatgaaa gacccggtgg
    13261 ccgccgaatt gttggccgcc tccggcccag ccaccccctc gatggcccct ttcccgaatt
    13321 tctccgggtt ccgggaaacc ctcttttcca ccgcgtaaga gatgagggaa gcgatcactg
    13381 ctcctcctcc cgggagtatc cccacgaaaa aacccaggac cgagccccgg agagtggccc
    13441 atttggcctc cgcccaatcc cggaggctga tgaagatacg atcaatcttt ttggttacca
    13501 gctgagtctt catggccttt tcggtgttga tcagaatctc ggcgatcccg aaaaggccca
    13561 tggccagagg aacgaaatcc agcccctcct gcaggggaag caccccgtag ctgaaacggg
    13621 ccactcccat gatggggtcg attccgacct catccagcat gaggccgagc accaccatga
    13681 tcagcccttt aaccatggag ctactcgcca ggtaggcagc caggaatagg ccgaacagcg
    13741 taagggcgaa atattcgggc ggtccgaact ggacggcaaa ctcggccagc gggggagcca
    13801 agaaattcag gccaacgatt ccgatcgttc cggcgataaa ggacccgatg gccgcaatgg
    13861 ccaaagccgg gcccgccctt ccctgcagtg ccatctggta cccgtcgatg caagtgacca
    13921 cggaagccgc ttccccggga atattcagga ggatggaagt ggtggagccc ccgtacatgg
    13981 ccccgtaaaa tatcccggag agcatgatga ttgcggagac aggcgggagc ttgaaggtga
    14041 tggggagcag gagagccacg gtagccgccg gcccgagtcc gggcaacacc cccaccagcg
    14101 taccgaccag ggttcccaaa aggcagtagg tcaggttttg ccaggtcagg gcgatggaga
    14161 aaccgtaata aaggccggtc agctgttcca aactaggctc ctcgacaagc tcataaaccc
    14221 cgaatgatcc ccctgggcaa ctggatggag aagaggaggt tgaaaaaata gacaaagacc
    14281 agggctaaac ccgccaggcc gacggccatc ggccatttct ttcgttccat cagcatcaac
    14341 cagagaagga aaagaagcaa agtgctgacg aggtatccca gccaactcaa aatcgcgcta
    14401 aaaaggcaga gaattccaat ggccaggagc gtgcggatta ggccagggga agggtgaagg
    14461 tcttcttctt gcttgaaggc acggaagccg gggaccagat atagaaggga aaggagaatg
    14521 agaacggagc ccagcccaaa aggaacaaag ccaggcccgg gccgactggc ctgccctaaa
    14581 ctgagctgga tcgcctgttg gcagacaaaa attcctaagg ccaggaatat gattcctgcc
    14641 agaatagacc ccgcgttcat gagggttatt tgatccccag gctcttggcg tcgtttttcg
    14701 cctcgttgta ttccttggtc acgaaggcct tgaagtcctt cggcccgatg taggcgggga
    14761 ggtcacccat tttgatgact aaatcaatcc atcccgggtc gcggcagagt ttttccaggc
    14821 ccgactccag ctttttgacg atcccttcgg gcaggttggg cggtccggaa acacccctcc
    14881 atcccatgac atccacttgg ggataaccca gctcggcaaa agtgggcaca tttggaactt
    14941 cttccatccg cttgggggcc aaggccaacg ctctcagctt tccagctttg agctgtgcat
    15001 agggctcgct gaggttcatg taggagaagt ggatgtttcc ccccagcata gccaccacct
    15061 gctccttgcc cgagttgaag ggaatcatgg tcacgctctg catatcgatc cccgcggcct
    15121 tcaggaattt ataggtggcc cagttgtgca tggacccgat tcccgaagtc ccgtatagaa
    15181 acttccccgg gtttttcttc acatcatcga ccaagtcctt gagcgtcttc cagggcgcct
    15241 cggttttaac caccaagacc gccggattga gctgggtacg gcaaatgaaa gtgacttcat
    15301 cgaattgaaa gggaagcccg gcctggaaag ccggggtgag ggtgttagaa ccgatggagg
    15361 cggaaagcag ggtgtatcca tcgggtttgg accggattac cgtttccatc cccaccgctc
    15421 ctcctgcgcc gggcttattc accacgatca tctgctgccc cacatacgtg ggggcatagc
    15481 gggccacggc ccgtgcagct tgatccgagg ctcctccggc tccccaaggg acgatgaggg
    15541 tgatgggctg attgggatag tctttatctt gagctgttcc ctgcaaaggg atgaacaagc
    15601 aggccgccac agcgatccaa cccgccgcaa aaatgggaaa gccctttttc atttttctct
    15661 cctttccgat ctccctcaga gaatccgacc tccaggggga aagcctgaaa ggatggctgg
    15721 tccctcggac tttcccgcgc tccgtaggta ttcctccaga actatttttt caaagactcg
    15781 attatctcga cctgcttctt cctctccccc atgatcttca ggaccatgtc gtagagggct
    15841 ttggactgat cttccatgct catggtgatg atctcgtggg ggtccgcact catggccttc
    15901 tggcagatgt ccacccactc cttgggaata gcgtcgattc ccttaaaggc tccggcgatg
    15961 gccccggcga tgctggcgat gcagtccgta tctcgaccta gggaagaccc ggcgatcatg
    16021 cactgtttgg gatcgccctt ggtaagccag aacatggcga aggactcgcc cactacttcc
    16081 agggagtctg tgggtaccct cccgccgaac ctcttccagt agagctcccg aacttccaac
    16141 atgtccggga cttgtttgac gtatccgatg cactcatcga tgatctggcc gatattttca
    16201 ttcacgtagg cctttcctgc ttcgatgatg gaatcgaccg tggcctgggg tttgaaggcc
    16261 tcggcaat
//
LOCUS       DASPRR010000034        10291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_328008, whole genome shotgun sequence.
ACCESSION   DASPRR010000034 DASPRR010000000
VERSION     DASPRR010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328008"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..411)
                     /locus_tag="VEL68_01320"
     CDS             complement(139..411)
                     /locus_tag="VEL68_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ34637.1"
                     /translation="MRVMINLLGTEIRLKQEVFLDPPSSDPKDVLRALKDQYGAPLER
                     FIKEDLSPAEGCTILVNGRNIGSLSLGETKIQDGDEITFTVLVAGG"
     gene            complement(424..2262)
                     /locus_tag="VEL68_01325"
     CDS             complement(424..2262)
                     /locus_tag="VEL68_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ34638.1"
                     /translation="MKGICGKLLEIDLTTGKTKETPIAGATMGKYLGGRGLGARLLFD
                     SLPAKTDPLSPENVLIFLTGPLTGSMVTGSSKFVVVTKSPLTHGWCDSYSSGRIALEL
                     KKAGYDGMMIRGRSNHPCYLRIDERGVEVRGAGSLWGKDSFETERMLKEMEKNPSVGV
                     SSIGPAGENLGKFACINSDLYRQAGRGGVGAVMGSKRLKAIVVKGSSGPALHDRKKLV
                     ELNRENYQRSRKSPVAEARMKYGTPLTLNITHAGGILPTKNFQFGTWEKALEKIDSLG
                     VHKSVKSHKACISCFVPCSLITEVGEGRYKGISVEGPEYETLGMFGSNLLIDSLPVII
                     QANILCDRLGLDTISAGNVIGFAMECFERGLISAKEAQGFEIRFGDAEASLALIDMMA
                     HRKGFGDILAEGVKAASERIGKDSGRFAMHVKGLEFPAYEPRGAFGSGLSYAVSPRGA
                     CHRRAWPPAKEILGGYPPYTVEGKAEMIKDLYDQNCVLHSLLVCDMPAKFIPLSLDDY
                     ANYFQAVTGESFSVNDFLTIAGRIETLIRMFNLREGFTRKDDTLPHRTLFEPLPDGPA
                     KGQFIGEENFNRMIDDYYACRGWDSQGVPTVATLRKYDLPREAPKG"
     gene            complement(2358..3296)
                     /locus_tag="VEL68_01330"
     CDS             complement(2358..3296)
                     /locus_tag="VEL68_01330"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ34639.1"
                     /translation="MDVRNISVIGLGLMGAPIASLLMKAGYRVTGFDIVKKQMSDLVP
                     LGLRAVNSPKAASQGTDLILLSLPNWEAVLAAVEGKDGVMEGARKGQIVVDTSTSPPW
                     ESKALGKRLAKKGIEWMDIPISGSSAQARVGNMVFMAGGKRAVYEKIKPVLDRIGKKT
                     VYAGKSGDGATLKLVINHTLYLNQAAAIEGLVLGLKAGLNPNVLFEVITSGAASSDLL
                     LARGKDMLQGNFSPKGPVVLANKDLALSLETARQLGVVLPVGALYQQLLLKAHYNGWD
                     REDATVVMRIYQQLAERAGVQRNPLPRDKNKNIKIK"
     gene            complement(3405..4466)
                     /locus_tag="VEL68_01335"
     CDS             complement(3405..4466)
                     /locus_tag="VEL68_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ34640.1"
                     /translation="MNPGDKVGLALIGIGSWSSIICGAVARSKRTKLVTCYSRTPEKR
                     DAYSKKYGCDQEKSYEELLKRKDVDGVILTTPNAAHAEHAVLAAQHGKHVYVEKPIAN
                     TLADGEKMVESCRKAGVVLSVGHDMRRLSGFRKMKGLMDTGAIGKPVMAESNFSSPMG
                     FELTPDKWRWYGDDSGCPAGALMTMGVHHADTLNHLFGPIEKVYAFFNKLYIPAEVED
                     VTMTIFQFQSGVLGYLGSSYATPRTNWVQVYGTEGKLSCSVSLPNLPFDEYLKVWTVV
                     DRYTTLQLFGKGKDQPEEIPLPIGDPILEEIDEFADCIQTGAQPETGGEGALVALALI
                     RAAIDSARTEKPVELIGNL"
     gene            4681..5067
                     /locus_tag="VEL68_01340"
     CDS             4681..5067
                     /locus_tag="VEL68_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ34641.1"
                     /translation="MGFYHQWGDFPEREISYLKGRPESSKLLVRILSSERMMVTNINA
                     RRGAFVPLHHHEAEQIILVLKGKIKAIMGKDSSQNLGPGGIWVVPSKHPHGVEYLEDT
                     EAIEVVSPPRLDNFVGYTISHTFFDE"
     gene            5300..6139
                     /locus_tag="VEL68_01345"
     CDS             5300..6139
                     /locus_tag="VEL68_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXZ34642.1"
                     /translation="MMVKVQVAAIQTKTFLEPDEQVRNIEGAWQYVTEAARRGAQFIC
                     FPETYPGPWKAPLGYSPVGPLEEMARKNDVYIVAGANCPVPGNPKKGYTSQVLVGPGG
                     LIGRYNRIIPRGPWIYKGGRFWDYDYQEADDLPVFDTPFCKVGILICSEVYSPELCRL
                     LALKGAEIIFLPAGICKYELHETWRNLIWSRAIENLAYTVTCQNILGAEHGLAMICSP
                     EEILIESLKPGIFTAECDFERIRWLREERDLYGPPMPWRTKPGILRDWRRPEVFRKNF
                     PDW"
     gene            6197..7210
                     /locus_tag="VEL68_01350"
     CDS             6197..7210
                     /locus_tag="VEL68_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ34643.1"
                     /translation="MKPRAIDIHTHVEFAGTLDILKKRYTEEEIFERFVVSATGRRSA
                     ELNRGIVAAIRDALRDPQKKIRDMDEAGIGFSVLSSTPFAFFYEVEDNLAVELARFQN
                     DKLAEMVKKFPDRFAAMATLPLQVPEEALKELNRAAKELGLRGVEIGSHIAKRELDDE
                     AFFPVYEALQGLDMPIFIHPHHVAGLDRLLEYYLNNLIGNPLDTTIAAANLIFGGVLE
                     KYPRLKIILAHAGGQFPYIIGRWEHGYKVRPECKDKVHQSPMAFFKNFYYDIITHNPD
                     ALRYLISLAGSDHVLLGTDYPYDMGDLNPVQTVAKLKEIKVRDRQRILRENAIALFGL
                     NTA"
     gene            complement(7397..8521)
                     /locus_tag="VEL68_01355"
     CDS             complement(7397..8521)
                     /locus_tag="VEL68_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ34644.1"
                     /translation="MAKKVRKEIGLAVVGCGTIGRIRTMLACNFPGVTWLGVCDLKEN
                     LAKKLAEDAQADFWTTDYKELLKRSEINAAIIATGENQHVAPTLQAVERRVDMLIEKP
                     LATDAKESQKILQAIQASGVDAVVGYTNRFRRRFLAIKERIKTKQIGEVTAATTRAFM
                     NRMVPIATVRRTEQRKTLTPMVVSGTHSLDMSLWLLEGKEPVEVYARSVDKVLGKWGT
                     KDATFGLFTMKDGTIWSMNISWALPTVWPGAVYSLEIGIVGTKGVITIDDTHRDLVLA
                     SEVAQGAGYKPEGFEPEYARHVDFLTSYPPGDVHLGQIWGAMRDETNSWLTRVCMGLP
                     TPHASAADGHRNLVLTMAMDLSAARGKALKLPIELDEFYA"
     gene            complement(8812..9903)
                     /gene="ugpC"
                     /locus_tag="VEL68_01360"
     CDS             complement(8812..9903)
                     /gene="ugpC"
                     /locus_tag="VEL68_01360"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXZ34645.1"
                     /translation="MAKVLMEKLNKHFGEVKAVRDFDLEIPDKEFVVLVGPSGCGKTT
                     TLRMVAGLEDITSGDIYIDDKVVNNLPPKDRDIAMVFQNYALYPHMTVYQNMAFGLTL
                     RKFPKPEIDQRVKDAAEILNIKELLDRKPKALSGGQRQRVAVGRAIVRKPKVFLFDEP
                     LSNLDAKLRVQMRVELKKLHDRLQTTIIYVTHDQVEAMTMGDRIVVMKDGLKQQVGPP
                     LELYFHPANKFVAGFIGSPAMNFIEGDQLSEGGALYFQGPGMKLKIPQEKASKLKDYS
                     EKRIIFGVRPEDLPEAPCAAPGETFTVVVEVMEPLGSEVYLDVKADDHSLIARAEPNT
                     TAKPHGSLCLRPTMENIHFFSIKDEKTLV"
     gene            complement(9908..>10291)
                     /locus_tag="VEL68_01365"
     CDS             complement(9908..>10291)
                     /locus_tag="VEL68_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ34646.1"
                     /translation="IPFCTWLLMGYFKSIPKEMEECAMIDGASRFRVFWQIVVPLALP
                     GILSSAIFAFTLSWNEFLYALTFISTNTQKTVPVGLITELVRGDVYHWGPLMTGALLA
                     SVPVALVYSFFVEHYVAGMTGALKE"
BASE COUNT         2223 a         2949 c         2702 g         2417 t
ORIGIN      
        1 tctcgcgcac ggatacctcc tccacggaaa aggtcttgca gcgaagatct atgcctagcc
       61 attttttgtt tgaaagaggc ttacgggatc cctttagact tccaagagca aataacacca
      121 gaagaattgt ccgacccctc agccacccgc caccaggacc gtgaaggtga tctcatcccc
      181 atcctggatt ttcgtctccc cgaggctgag ggacccgatg tttctgccat tcaccagaat
      241 cgtgcaacct tccgccgggg aaaggtcctc ttttatgaat cgttctaaag gagccccgta
      301 ctgatctttc agcgccctca aaacatcttt cgggtccgaa gacggcgggt ccaagaagac
      361 ctcctgcttc agccgaatct ccgtacccaa gagattgatc atgactctca tggtttcagc
      421 caatcatcct ttgggggctt cccgggggag atcatatttc cgaagggtcg ccacggtagg
      481 gactccctgg gaatcccatc ctcggcaggc atagtagtca tcgatcattc gattgaaatt
      541 ctcctccccg atgaactgcc ctttggccgg gccgtcgggc aaaggctcaa agagcgtccg
      601 gtggggaagg gtatcgtcct tccgggtaaa cccctcgcgg agattgaaca tccgaatcag
      661 ggtttcaatc ctgccggcaa tggtcaggaa atcatttacc gaaaaggact ccccggtgac
      721 cgcctgaaaa tagttggcat aatcgtccag agataaagga atgaacttgg ccggcatgtc
      781 gcagaccagg agcgaatgga gaacgcagtt ttgatcgtac agatctttta tcatctccgc
      841 cttgccttcc acggtataag gcgggtatcc gccgagaatc tccttggccg ggggccaggc
      901 ccttcgatgg caagcccccc tggggctgac ggcataggaa agcccggaac caaaggcccc
      961 tctcggttca taggcaggga attccagccc tttgacgtgc atggcgaatc ggccggaatc
     1021 ctttccgatg cgctcggagg ccgccttcac cccctcggcg aggatgtccc cgaatccttt
     1081 ccggtgagcc atcatatcaa tcaatgctaa actggcttcc gcgtcgccaa aacgaatttc
     1141 gaacccctgg gcttctttcg ccgagatcag tcccctttcg aagcactcca tggcgaaccc
     1201 gatcacattc cccgcggata tggtgtccag tcccagccga tcacagagga tgttggcctg
     1261 aatgatgacc ggaagggaat cgatgagcag attggacccg aacatcccca gggtctcata
     1321 ttccgggcct tccacggaaa ttcctttgta ccggccttcc ccgacctcgg tgatcaggct
     1381 gcaagggaca aaacaggaga tgcaggcttt gtgggatttg acactcttgt ggacccctag
     1441 ggaatcgatc ttctccagag ccttttccca ggtaccgaat tgaaaatttt tcgtgggcag
     1501 gatccccccc gcatgggtga tgttcagggt cagaggggtt ccatatttca tccgtgcttc
     1561 ggccaccggg ctttttcggg aacgctggta gttttcccgg ttcagctcaa cgagcttttt
     1621 ccggtcgtgg agggccgggc cgctggaccc cttgaccacg atggccttga gtctcttgga
     1681 gcccatgacc gccccgaccc cgcccctccc cgcctggcgg tataaatcgc tgttgatgca
     1741 ggcaaattta cccaaatttt ctccggccgg tccgatggag gaaactccca cggaaggatt
     1801 tttctccatc tccttgagca tcctttccgt ctcgaaactg tccttgcccc aaagtgaacc
     1861 cgctccccgg acctccacgc cgcgctcatc gatcctcagg tagcagggat ggttcgacct
     1921 tccccgaatc atcatcccgt cgtaacccgc ctttttaagt tccagcgcaa tccttccgct
     1981 ggaatagctg tcgcaccatc cgtgagtaag gggcgacttg gtcaccacta cgaatttgga
     2041 cgagccggtt accatcgaac cggtgagggg cccggtcagg aagatcagca cgttttcagg
     2101 ggaaaggggg tccgttttgg cgggaaggga atcaaagagc aaccttgctc ccaggcctct
     2161 tcctcccagg tactttccca tcgttgcgcc cgcgatcgga gtttccttcg tctttccggt
     2221 agtcagatca atttccagca acttgccgca tatccctttc ataaactcct cttcatttgc
     2281 caaaccgtaa ggcctgatcc ccgaccaggc cttctgcttg gcgcgttcgg ggatcgcacc
     2341 taacatttca ggctattcta ttttattttt atgtttttgt ttttatctct tggcaaaggg
     2401 tttcgttgca ctcccgccct ctcagccagc tgttgataga ttctcatgac caccgtggca
     2461 tcctcccgat cccagccgtt ataatgagcc ttcagcagaa gctgttggta caatgctccc
     2521 acgggtaaga cgactcccag ttgccttgcc gtttccaggg aaagggcaag gtccttgttg
     2581 gccagaacga caggcccctt gggggagaaa ttgccctgga gcatatcttt tccccgggcg
     2641 agcagcaaat cgctcgaggc ggcccctgag gtgatcacct cgaacaggac gttgggattc
     2701 aggccggctt tcaagcccag aaccagcccc tcgatggccg cagcctggtt gaggtatagg
     2761 gtgtggttga tgaccagctt cagcgtcgcc ccatccccgc tctttcccgc ataaactgtt
     2821 tttttaccga tccggtcgag aacgggcttg atcttttcat agacggctct tttccccccc
     2881 gccataaaga ccatatttcc cacccgggcc tgcgcagagg aaccggagat gggaatatcc
     2941 atccactcga ttcctttctt cgccaatctc tttcccaaag ctttactctc ccacggaggg
     3001 gaggtactgg tgtccaccac gatctggcct ttccgggctc cttccatcac cccatccttt
     3061 ccttccaccg cggcgaggac cgcctcccag ttcggcaggg ataggagaat gaggtccgtt
     3121 ccttgggacg ctgctttggg ggaattcacc gccctcaacc cgagggggac taggtcggac
     3181 atctgcttct tgacgatgtc aaaaccggtc acccggtatc cagctttcat cagcaaggag
     3241 gcgatggggg ctcccatcag tcccaggcca atgaccgaaa tgttcctgac atccacggtt
     3301 tatctccccc ttttcgatcc ttgccaatat caatgcataa aaaaaattta gggtgcgtgc
     3361 cccgaagcat cacgccttgg cgtgacctga tcggggatca ggccctacag attgcctatc
     3421 agttccaccg gtttctccgt tcgtgccgag tctatggccg ctcgaatcaa agccagggca
     3481 accagagctc cctccccacc tgtttctggc tgggctcctg tttgaatgca gtcggcaaac
     3541 tcgtcaatct cttccaggat cgggtctcca atcggcaaag ggatttcttc tggctggtct
     3601 tttccttttc cgaatagctg cagggtagta taccgatcca caacggtcca gaccttcagg
     3661 tattcatcaa aaggcaaatt gggcagagag acggagcagg agagtttccc ttccgtgccg
     3721 tagacctgta cccagtttgt tctcggagtt gcataggacg atccgagata acccaggacg
     3781 cctgattgga attggaatat agtcatggtc acatcttcaa cctccgctgg aatgtagagc
     3841 ttgttgaaga aagcgtagac cttctcaatc ggtccaaaaa gatgattgag cgtatccgcg
     3901 tgatgaaccc ccatggtcat gagcgctcct gcaggacatc ccgaatcgtc tccataccag
     3961 cgccatttgt caggagtcaa ttcaaatccc atcggggaag agaaattgga ctccgccatg
     4021 accggtttcc cgatcgcgcc tgtatccatg aggcctttca tctttcggaa tccggataag
     4081 cggcgcatat catggccaac ggataggacg actccggctt tccggcacga ttcaaccatt
     4141 ttttccccat ccgctaacgt attggcaatg ggtttttcta cgtagacatg cttcccgtgt
     4201 tgagccgcta aaacggcatg ctcggcatgg gccgcattgg gagtggtcag gatcactcca
     4261 tcgacatcct tccgcttcaa cagttcttcg tagctcttct cttgatcaca tccatatttt
     4321 ttactatagg catcgcgttt ctcgggtgtc ctcgaataac aggttaccag ttttgttctc
     4381 ttgctcctcg ccacggcacc gcaaatgata ctcgaccaac tcccgatccc gatgagagcg
     4441 agccccacct tgtcccctgg attcatgaat cctttctcct tttcgaggac ggctttcctg
     4501 aaccttcctc cgatgggaat catttaatcc ccattctaat ccctgattgt cctttgtcaa
     4561 attatttatc agccctgttt tctcctgtct gtcccacccc tcaccctgga caaacttctc
     4621 catttttgtt aaaaaagttt gtttcctgat ttcacttcaa agtagaaggg gggccctaat
     4681 atgggatttt atcatcaatg gggagatttt ccggagagag aaatcagtta cttgaagggt
     4741 cgtccggaat cttccaagtt gcttgtccgc atcctgagca gcgagaggat gatggttacg
     4801 aacatcaacg cccggcgagg agccttcgtg cccctccatc accatgaggc cgagcagatt
     4861 atcctggtct taaagggaaa gatcaaggcc atcatgggaa aagattcgtc tcaaaacctc
     4921 gggcccggcg gcatctgggt cgtcccctct aagcaccccc atggcgtcga atacctggaa
     4981 gatacggaag ccatcgaagt agtgagccct cctcgcctgg acaattttgt tggttataca
     5041 atttcccata ccttcttcga tgaatgagaa gaaaatgaaa gggatctgaa aattcggcca
     5101 tctcccctgg atcgcccctc ccctagagcg gggaaaagat gcaatcatgg ccagttttca
     5161 agcacgcgca tcacccgcgc atcaccttgc taaaatggga taccggatga tatgctggag
     5221 gcctcagtcg ggggagaatt gcgatggatc acccgaggaa ggcagaagaa gccgaaggaa
     5281 acccggattc gaggaggcga tgatggtgaa agttcaagtg gccgcgattc aaaccaaaac
     5341 gtttctggaa ccggatgagc aagtacgaaa tatcgaaggg gcatggcagt atgtgactga
     5401 ggcggcaagg cggggggccc agttcatttg ctttccggag acctatccgg gcccctggaa
     5461 ggcgcccctt ggctattcac ccgttggccc actagaggaa atggccagga agaatgacgt
     5521 ctatattgta gctggagcca attgtcccgt accgggaaat ccgaagaagg gctacacctc
     5581 ccaggttctt gtcgggccgg ggggcttgat cggaagatac aatcgcatca ttccccgggg
     5641 cccctggatt tataagggag gacgtttctg ggattatgat taccaggagg cggacgacct
     5701 tcccgttttc gataccccct tctgcaaggt tggaatcctg atctgcagcg aagtctattc
     5761 gccggaactc tgccgccttc tggctctaaa aggagcggag atcatctttt tgcccgcagg
     5821 catttgcaaa tatgagcttc atgagacctg gcggaatctg atttggtcga gggccatcga
     5881 aaacctcgcc tacacggtga cctgccagaa tattctgggg gccgagcatg gactcgccat
     5941 gatctgcagc cccgaagaaa tcctgatcga gtcgctcaag cctggaattt tcaccgccga
     6001 atgtgacttc gaacggattc gatggctccg ggaggaaagg gacctttacg ggccgcccat
     6061 gccctggagg acgaagccgg gtatccttcg ggactggcgc cggcccgagg tgttccggaa
     6121 aaactttccc gactggtgac cgtgagataa ggaaggttga agagataaaa tcgaatcggg
     6181 atgagaggag agactgatga aacccagagc catcgacatt catacccatg tggagtttgc
     6241 gggtaccctt gatattctga agaaacggta cacggaggag gagatcttcg aacgctttgt
     6301 cgtttcggcc accggccgcc gttccgcgga actaaaccgg gggatcgtcg cggccattcg
     6361 cgacgccctg agggaccccc agaaaaagat tcgggatatg gatgaagcgg gaattgggtt
     6421 ttccgtgctc tcctccactc ccttcgcctt tttctacgag gtggaggaca atctggccgt
     6481 tgaactggcg aggtttcaaa atgacaaact cgcagaaatg gtgaaaaagt ttccggaccg
     6541 ctttgccgcc atggccaccc tccccctgca ggttcccgaa gaagcgttaa aggaactgaa
     6601 cagggcggca aaggaattgg gtttgcgggg cgtggaaatc ggctcccaca tagcgaagcg
     6661 ggaattggat gatgaagcct tcttcccggt gtatgaagcc ctccaaggcc tggacatgcc
     6721 cattttcatc cacccgcacc acgtggccgg cttagaccgg ctcctggaat attatctgaa
     6781 caatctcatc ggcaatccgc tggacacgac cattgccgcg gccaacttga ttttcggcgg
     6841 ggtcctggag aaatacccgc gcctcaagat catcctggct cacgctggcg ggcaattccc
     6901 ttacatcatc ggaaggtggg aacatgggta caaggtccgg ccggagtgca aggacaaggt
     6961 ccaccaatcc cccatggctt tttttaagaa cttctattac gacatcatca cccataaccc
     7021 cgacgccctt cggtacctga tctcccttgc cgggagcgac catgtccttt tgggcaccga
     7081 ctatccttac gatatggggg acctgaatcc ggtgcagacg gtagccaagc tcaaagagat
     7141 caaggtcagg gaccgtcaaa ggatcctgag agagaacgcc atcgccctct tcgggttgaa
     7201 tactgcctag accgatattt ctctgccaat ggcagaatga tccccgcctt ttaaaaaaaa
     7261 caaggacgtc cttttcccca tcccacaagg tttaaatcgg aatcggaaat taattaaatt
     7321 ttcttagggc atcctgcatt ttcgcattct ttaattcttg tcggcccgtc gcccgaatga
     7381 atcccgggtc tctcgttcag gcgtagaatt catcgagctc gatgggcagc ttcaaagcct
     7441 tcccgcgcgc cgcagacaaa tccatggcca tggtgagaac taggttccgg tgtccatcgg
     7501 ccgcggaggc gtggggcgtc ggcaagccca tgcaaacccg ggtcagccag gaattggtct
     7561 cgtcccgcat ggccccccag atctgcccca aatgaacatc tcccggagga tagctggtga
     7621 ggaaatcaac gtgacgcgcg tattccggct caaatccttc gggcttgtac ccggccccct
     7681 gagcaacttc cgatgccagc accaggtcac gatgggtatc gtcgatggta atgaccccct
     7741 tggttccgac gatcccgatt tccagggaat aaaccgcacc cggccaaacg gttggcaggg
     7801 cccagctaat gttcatgctc caaatcgttc catccttcat cgtgaagaga ccgaaggtgg
     7861 cgtctttcgt cccccatttt cccaggacct tgtcgacgga ccgggcgtag acttcaaccg
     7921 gttcctttcc ctccaacagc cacagggaca tgtcgaggct gtgggtcccg gatacgacca
     7981 taggcgtcaa cgtctttcgc tgctcggtgc gccgcacggt ggcgatgggt accatgcggt
     8041 tcatgaatgc ccgggtcgtg gccgcggtga cctcgccgat ctgctttgtc ttaatccttt
     8101 ccttgatggc caggaagcgg cgccggaaac gattggtgta tccgacgacg gcatccacac
     8161 cactcgcttg gattgcttgg aggatttttt gggattcctt ggcatccgtg gccaggggtt
     8221 tttcgatcag catatccacg cggcgttcca ccgcttgcag ggtgggggca acgtgttgat
     8281 tttcaccggt agcgataatc gcggcattga tttcagagcg cttcagcagc tctttataat
     8341 cagtggtcca gaaatcagcc tgggcatctt cggcgagctt tttggccagg ttttccttca
     8401 ggtcacatac acccagccag gtcaccccgg gaaagttgca agctaacatc gtgcgaatcc
     8461 gcccaatcgt gccgcatccg acgactgcca acccaatttc ttttcttact ttttttgcca
     8521 tttccgctcc tttcttaagc cgatttccct ctccggtccc acggctgaga tcgggtcacc
     8581 cttgatgcga accgctccat tttgaatggg cgatcgtccc gtcccatcga tgagcaaagt
     8641 ccatttgatg actataaaat tcatcttctt gaggtccgtt tttggctgaa ataaagttgt
     8701 ctacttatgg cgagagtcga accgggggct gatgcagaaa gcagagcgca aagcgttgag
     8761 gactcccgat cctattttaa cgctatgcgc tctgcgcgaa gcgctttgag gttacacgag
     8821 tgtcttttcg tccttgatgc tgaagaagtg gatattctcc atggtgggcc tcaggcaaag
     8881 actgccgtgg ggcttggccg tggtgttggg ctcggcccgg gcgatgagcg aatgatcatc
     8941 ggctttcacg tccaggtaca cttcgcttcc cagaggctcc atgacttcca cgaccaccgt
     9001 gaaggtctcc ccgggggctg cgcagggggc ttccggcagg tcttccgggc ggacgccgaa
     9061 gattatccgc ttttcggagt aatccttcag cttgctcgcc ttttcctggg ggatcttcaa
     9121 tttcatcccc ggaccctgga aataaagcgc cccgccttcg gaaagctgat ccccttcaat
     9181 aaagttcatg gccgggctgc cgatgaaacc ggccacgaat ttgttggccg gatggaaata
     9241 aagttcgagg ggtggaccga cctgctgttt cagcccgtcc ttcatcacca cgatccggtc
     9301 tcccatggtc atggcttcaa cctggtcgtg ggtcacgtag atgatagttg tctggagccg
     9361 gtcatggagc tttttcaact ccacccgcat ctgcacccgc agcttggcat ccaggttgga
     9421 gaggggctcg tcgaaaagga agaccttggg cttgcgcacg atggcccggc ccacggcaac
     9481 ccgctggcgc tgcccacccg aaagagcctt cggcttgcgg tccagaagct ccttgatgtt
     9541 caggatttcc gccgcatcct tgacccgctg gtcgatctcg ggcttgggaa atttgcgcag
     9601 ggtgaggccg aaggccatgt tctggtaaac ggtcatgtgg gggtagagag cgtaattctg
     9661 gaagaccatg gcgatgtccc ggtccttggg ggggaggttg ttgaccactt tgtcgtcgat
     9721 gtagatatcc cccgaggtga tgtcctccag gcccgccacc atgcgcaggg tggtggtctt
     9781 gccgcagccg ctgggaccga ccaggaccac gaactccttg tccgggatct ccaagtcgaa
     9841 atccctcact gctttgactt ccccaaaatg cttgttcagt ttctccatca gaactttagc
     9901 catagtttca ctcctttagc gcgccggtca tgccggcgac atagtgttcc acgaaaaatg
     9961 agtataccag agccacgggt acggatgcca gcagggctcc ggtcatgagc ggaccccagt
    10021 gatagacatc gccccggacc aattcagtaa tcaggcccac gggaacggtc ttttgcgtgt
    10081 tggtcgatat aaaggtcaga gcatagagaa attcgttcca ggaaagggtg aaggcaaaga
    10141 tcgccgagga cagaattccc ggcagggcca gggggacgac gatctgccag aaaacccgga
    10201 accggctggc cccgtcgatc atggcgcact cttccatctc tttgggaata gacttgaaat
    10261 atcccatgag cagccaggtg cagaagggga t
//
LOCUS       DASPRR010000035         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1301173, whole genome shotgun sequence.
ACCESSION   DASPRR010000035 DASPRR010000000
VERSION     DASPRR010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301173"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VEL68_01370"
     CDS             <1..729
                     /locus_tag="VEL68_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXZ34647.1"
                     /translation="FNLRPAEKPPLRLDLEESEKEKKEEGTPWERRLQWKESLDAVKE
                     KASVGLPLLAPSSAVPSPLDLTSHRLDEWIEPGASRGKGEEGGPFFGLAFHGVMERLD
                     LSGTDNLKELCRIQALEYSIPGLGEKLEAMVRRSLGHPLMERVKRAKRLFREVPFSVS
                     LDGSLVEGKIDLLFNEGSGWVIVDYKTDEVFGEALEQRFQSYREQGLCYARAVREAAG
                     CEVKEVAFFFVRSGEVRLIKTNEI"
     gene            complement(802..1647)
                     /locus_tag="VEL68_01375"
     CDS             complement(802..1647)
                     /locus_tag="VEL68_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HXZ34648.1"
                     /translation="MRWEDQERSENVEDRRGMRLSRGVMGGGIGTVILVLAALYFGVD
                     PSVILNSVPMTGPPAETRQQKPGPDPMREFVSVVLADTEKTWHEVFRRYGKTYEEPKL
                     VLFAGAVQSACGFAQEAMGPFYCPEDHRVYIDLAFYEDLKNRFKAPGDFAQAYVIAHE
                     IGHHVQNLLGITEKVQAAMQRSSRAEANHLSVRMELQADCLAGVWGYHADKSRHILEA
                     GDLEEALRAASAIGDDRIMKQTRGTVVPDAFTHGTSEQRVRWFQRGFETGDMNQCNTF
                     KAGDV"
     gene            1885..>3441
                     /locus_tag="VEL68_01380"
     CDS             1885..>3441
                     /locus_tag="VEL68_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXZ34649.1"
                     /translation="MKSYFKLGLALAGVLIAVGLGLSSCSKEKTTYTGSVSCRSCHEK
                     FYQLWSTSYHGLAMQPYTAELARKKLSPMSQSIAIGKAAYRVDIEGKTGYVREKGADG
                     EKKYEIQHVMGGKNVFYFLTPMEKGRLQVLPLAYDVNRKTWYDTTASHLRHSLDRRDE
                     ALDWREWPLTFNTMCYNCHVSQLSTNYDLKTDTYNTVWTEPGINCETCHGPAAEHIKV
                     LEKSPKEKVPEEVKMIRWKTFTAEQKNDACSPCHAKMRPVTPSFTPGDRYFDHFDLVT
                     LEDSDFYPDGRDLGENFTLTLWRMSPCVKSGKLDCIHCHTSSGRYRFKQDETANNSCL
                     PCHAERVQNAPTHTNHKAGSVGNKCVSCHMPMTEFAFMRRSDHSMLPPAPTATLLLQS
                     PNACNICHKDRDARWADDWVRKWRNRDYQAPLLYRTGLIDAARKRDWSRLTEMLRYTA
                     AKNRDEIVATSLIRLLQACPKGEKWPAILQALKDPSPLVRSAAAAALATYSSPQAQEA
                     LLEASGDDFRL"
BASE COUNT          756 a         1018 c          934 g          733 t
ORIGIN      
        1 ttcaacctcc gaccggcgga gaaaccaccc ctgcgattgg acctggagga atcggaaaaa
       61 gaaaaaaaag aagaggggac tccctgggaa cgacgtcttc aatggaaaga atccttggat
      121 gcggtcaaag aaaaggcttc cgtaggcctt cccctattgg ccccatcctc cgcagttcct
      181 tcgcccctcg acctcacatc ccaccgcttg gatgagtgga tcgaaccggg ggcttctcgc
      241 gggaaagggg aggaaggagg tccttttttc ggccttgctt tccacggtgt catggagcgt
      301 ttggacctgt ccggcacgga caatttaaag gagctctgcc ggatccaagc ccttgaatac
      361 tccatccctg gattgggaga gaagttggaa gccatggtgc ggcgatccct ggggcaccct
      421 ctgatggaga gggtcaagag agcgaagcgt ttattccggg aagttccctt ttccgtctct
      481 ctggatggca gtctggttga ggggaaaatc gatcttctct tcaatgaggg gagcggctgg
      541 gtgatcgtgg attacaaaac cgacgaagtt ttcggggaag ccttggagca gcgctttcaa
      601 tcctaccggg agcagggcct ctgctacgcc cgtgcagtcc gggaagcagc tggctgcgag
      661 gtcaaggaag tcgctttttt ctttgtccga tccggggagg tccggttgat caaaaccaat
      721 gaaatttgaa ttctgaatta ttgaaaggtc ggaccttaaa ctacggcatg cttttccccg
      781 tattcaccac tcgatggttg attaaacgtc acccgccttg aaggtattgc actgattcat
      841 atcacccgtc tcaaagcccc tctggaacca gcgcaccctc tgctccgagg tgccgtgggt
      901 gaaggcatcg ggaacaaccg tgccgcgggt ctgtttcatg atgcggtcgt cgccgatcgc
      961 gctggccgcg cgcagcgcct cttccaagtc gccggcttcc aggatatggc gtgatttatc
     1021 cgcatgatac ccccagacgc cggccaggca gtcggcctgc agttccatcc tcacggataa
     1081 gtggttggcc tcggccctgc ttgagcgctg catagcagcc tggactttct ctgtgatccc
     1141 cagtaggttc tgaacatggt ggccgatctc gtgggcgatc acgtaggcct gggcaaaatc
     1201 gccgggcgcc ttgaagcggt ttttcaggtc ctcgtaaaag gccaagtcga tatagacccg
     1261 gtgatcctcg gggcaataga agggtcccat ggcttcctgg gcgaagccgc aggcggactg
     1321 caccgcaccg gcgaagagca cgagcttagg ctcctcatag gttttcccgt agcgacggaa
     1381 tacctcgtgc caggtctttt ccgtgtccgc caggacgacc gaaacaaact cgcgcatggg
     1441 gtctggtccg ggcttctgtt gccgggtctc ggccggggga ccagtcatag gaaccgaatt
     1501 caggatcacc gaggggtcga cgccgaaata cagggccgcc agcaccagaa ttacggtgcc
     1561 gatcccgccc cccatcaccc cgcgggatag gcgcatcccc cgccggtcct ccacattttc
     1621 gcttctttct tgatcttccc agcgcataaa aggtctcctt tggggtatcc accttctatc
     1681 gggtcttcct gcttccgccc attcattcct ctttcgccgt gaataaaata ttttaatatt
     1741 tgcatgagag tctgtacagg ggtaagggct attttttttg ccaatctgcc tcttttaggc
     1801 aagacttccg cttattgaat tttatcgcag aaatcattac catgggggca aactttcatt
     1861 aatgtccaga gggaggcaag agtcttgaaa tcatatttca aattggggtt ggcactggcg
     1921 ggagttctga tcgccgtcgg tttggggctt tcatcctgct ccaaagagaa aaccacctac
     1981 accggctcgg tcagttgccg cagttgccac gagaaattct atcagctctg gtcgacttcc
     2041 taccacggtt tggccatgca gccttacacg gcggaattag cccggaaaaa gctgtcgccc
     2101 atgtctcaat ccatcgccat cggcaaagcc gcctaccggg tagacatcga agggaagacc
     2161 gggtatgtgc gggaaaaggg agctgacggg gaaaagaaat acgaaatcca gcacgtcatg
     2221 ggaggcaaga acgttttcta ttttttgacc cccatggaaa agggccggct gcaggtcctc
     2281 ccgcttgcct atgacgtcaa tcggaaaacc tggtacgaca ccaccgccag tcacctgcgc
     2341 cattccctcg accggaggga tgaagcgctc gattggagag aatggccact gactttcaac
     2401 accatgtgct acaattgcca cgtaagccag ctctccacca actatgacct gaaaaccgat
     2461 acctacaata cggtctggac ggagccggga atcaattgcg aaacctgcca tggtcccgcg
     2521 gccgaacaca tcaaagtgtt agagaaatca cccaaggaga aggttcctga agaggtgaaa
     2581 atgatccgtt ggaaaacctt caccgccgag caaaaaaacg atgcctgctc cccctgccac
     2641 gccaaaatgc gccctgttac cccttctttc acaccggggg atcgctattt cgaccacttt
     2701 gatttagtca ccctggaaga ctccgatttt tacccggacg gtcgggacct gggcgaaaac
     2761 ttcaccctga ccctctggcg gatgagcccg tgcgtgaaat cggggaaact ggactgcatc
     2821 cactgccaca cctccagcgg ccgctaccgc ttcaaacagg acgagacggc caacaactcc
     2881 tgcctaccct gccacgcgga gcgggtccag aatgcaccga cccacacgaa tcataaagcg
     2941 gggagcgtgg gcaataaatg cgtgtcctgc catatgccca tgaccgagtt tgctttcatg
     3001 cgccgcagcg accactccat gctacccccg gcgcccacgg caaccctgct tcttcaatcc
     3061 cctaacgcct gcaacatctg ccacaaggac cgggatgcac ggtgggccga cgactgggtc
     3121 cgtaaatggc ggaaccgcga ctaccaggcg cctctcctgt accgtaccgg cctgatcgac
     3181 gctgcccgca agagagactg gtctcgtctg acggagatgc tccggtacac tgccgccaag
     3241 aaccgggatg aaatcgtggc tacctcgttg atccgcctcc tccaggcctg tccaaaggga
     3301 gaaaaatggc cggctatcct ccaggcgctc aaagacccgt ctcccctggt tcgttccgct
     3361 gctgccgcag ccttggccac atattcgtcc ccgcaggccc aggaggcgct cctcgaggcc
     3421 tcgggggacg actttcggct g
//
LOCUS       DASPRR010000036        11904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2107257, whole genome shotgun sequence.
ACCESSION   DASPRR010000036 DASPRR010000000
VERSION     DASPRR010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107257"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /gene="proS"
                     /locus_tag="VEL68_01385"
     CDS             <1..1450
                     /gene="proS"
                     /locus_tag="VEL68_01385"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXZ34650.1"
                     /translation="YEIGDEMFRLKDRNQRDMVLGMTHEEIIAWLAAREVRSYRDLPQ
                     IWYQIQTKLRDEARPKSGVLRTREFLMKDSYNLDLDAAGLDQSYQLHYDAYVKVFRRC
                     GVKFYVVESDPGMMGGAGAHEFMAPSEAGEDEVARCENCGYAANVELAKSTSFPPDFP
                     SWKFEEVATPDSRTIEEVCSYLGISPQLTIKSLLLMANDKPVMALVRGDQQLHEKKFR
                     SLVGEFRPAHREEVKQFTGVEAGFLGPARLSPKQPISMIADTSLKEGVFVAGANREGY
                     HLRGVVPGEHFSARFADIHLAMSGDACPQCQAPLKVEKTIEIGNIFKLGTKYSIPLKA
                     LYLDRQGQEKPIVMGSYGIGPARIIAAAVEQNHDSNGIIFPLSITPFEVHLLPVNLKQ
                     GTIGAEAEKLYQGLSTAKVETLFDDREEAPGVKFKDADLLGAPLRLTLSAKTLKEKAA
                     EIKVRRSGEVHMIPLDRAVSWVRDWIDDQRKK"
     gene            1553..3736
                     /locus_tag="VEL68_01390"
     CDS             1553..3736
                     /locus_tag="VEL68_01390"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXZ34651.1"
                     /translation="MIRLNDILEKVSRNFPGANLSLIEKAYVYTAKVHQGQVRLSGEP
                     YLIHPLAVAAILADMKMDIATVAAGLLHDTVEDTFATLEEIKDLFGPEIAALVDGLSK
                     ISKMTLATHEEQQSENFRKMLLAMSRDIRIVLIKLADRLHNMRTLQHLPPEDQNKIAQ
                     ETLDIYAPIAGRLGIDRIKTELEDLTLPYLHPEEYRRLMAAIAWKKEEREKYIQEVSS
                     IISEKLASYGLVGKVSGRLKHFYSIYRKMKAQNLEFEQVYDIIAFRMILSSVKDCYEA
                     LGIIHSLWKPIPGRFKDYIAMPKANGYQSLQTTVIGPYGERVEIQIRTEEMNRTAEEG
                     IAAHWQYKEGKAFDPKDTRQIAWVRQLLEWHQDLRDPREFIETVKIDLFPDEVYVFTP
                     RGDVKQFPIGATPVDFAYSIHTEIGHQCVGAKVNGKIVPLRYQLQNGDRVEIVTSPGH
                     RPSKDWLKHVKTSRARTKIRQWVKAEARERSVDLGREICEREFKKYHLDFPKFLKSED
                     LKKVMGDFSFQSVDDLMAEIGYGNISCKQVVGKLLPPDKLEKGKVEESRLKRLADKIR
                     REPSGIQIRGIRDMMVRFGKCCNPLPGDRITGYITRGRGVTVHTADCPNVLGGDPDRL
                     IQVSWNLQEKAVHAVRVRVTCNDKKGLLAEIGAALASSEINILQAHVVTTEDRKAICN
                     FELEVRDLKHLQAAFRTLTKLKDVLKVERMRGATSGEREEPGAEP"
     gene            complement(3798..3989)
                     /gene="rpmB"
                     /locus_tag="VEL68_01395"
     CDS             complement(3798..3989)
                     /gene="rpmB"
                     /locus_tag="VEL68_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXZ34652.1"
                     /translation="MARACEVCGKSPKVGHKVSHSNIKSKRRWYPNLQRVHAIQNGTS
                     RRIRVCTRCLRSGRVVKAI"
     gene            complement(4124..4783)
                     /gene="purN"
                     /locus_tag="VEL68_01400"
     CDS             complement(4124..4783)
                     /gene="purN"
                     /locus_tag="VEL68_01400"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXZ34653.1"
                     /translation="MVNLGVLVSGSGSNLQAIIDNIEAGRVDARIKIVISNLPEVYAL
                     ERANKHGIPSLVILHKGLKREAYDQKVVEALKAHEVELVILAGFMRIITSVLLRAFPM
                     RVMNIHPALLPAFPGTHVWQTQIDYGVKFAGCTVHFVDEGTDTGPIIIQGVVPVYDDD
                     TADTLNARILKREHKIYSQAIQMYARGQLEIQGRRVLAKGVPQVPDSFMINPPPSHLD
                     P"
     gene            complement(4792..5838)
                     /gene="purM"
                     /locus_tag="VEL68_01405"
     CDS             complement(4792..5838)
                     /gene="purM"
                     /locus_tag="VEL68_01405"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXZ34654.1"
                     /translation="MGKRMTYRGAGVDIDAGNKFVDLIRPLVRKTFRPEVVTDIGGFG
                     GLFALRAKKNKNPILVSSTDGVGTKLKVAFTTGKHDTVGIDLVAMCVNDVVVLGAEPL
                     FLLDYLATGKLALGVSTRIIRGVARGCIQAGCALIGGETAEMPSFYREGEYDLAGFTV
                     GVVEKSRIIDGSAIRAGDRLVGIASSGLHSNGYSLARKVFFDRMKLKPSSRVRGLSRP
                     IGEELLIPTKIYVKPLLNLIQSFPVHGMAHITGGGITDNLPRVIPAGCKAVIRKGSWP
                     VHPIFKVLQEAGNVAEEEMLRTFNNGIGMILAVPPRVVEGVIARLRRWREKAYPIGEI
                     VKKVNPKDSPITYL"
     gene            6076..8553
                     /gene="recG"
                     /locus_tag="VEL68_01410"
     CDS             6076..8553
                     /gene="recG"
                     /locus_tag="VEL68_01410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXZ34655.1"
                     /translation="MIPSELEEILAAIEKPIHFASGHDFANLKTVKGLEPYLAHWLDK
                     ATALALSPRREDLLQRLRVQVQGFEGLDLDGKKARVVEVQKIIQDLKCGDGVSLPCQP
                     WPTQAEFHQSRKELQTSIQFIKGVGPRLSEVLKKKNILTVEDALYFLPRLYQDRRQIK
                     TISQLAVGKIETVMGTVLKADMTFYGRRRTFELTVGDETGTLIAKWFNFNPRFMKGRF
                     KKGMRLILFGEVKFYQFQKEIHHPELEILEEESESPDGNAAPPPGKEESLHFGRVVPV
                     YSETEGLYQRQRLIRRIMKNAVDLFAGKEFSGIPEEVCGRQSLIPLSEAFRRAHYPDA
                     DEDLSLLNEGKSSAHRRLIFDEFFFLELGLALRRSGTLMEKGIPFPISHRHTRRLREI
                     LPFSLTPAQERVLAEIESDMRRPHPMNRLLQGDVGSGKTIVALLSGLMAIEGGYQVAI
                     MAPTEILAEQHYLNIRPLVEKLGFQAALLTSSLKKSERDSFLLRIRKGEIHVVIGTHA
                     LIQEGVEFQHLGLAIIDEQHKFGVLQRATLKKKGLNPDVLVMTATPIPRTLAMTLYGD
                     LEVSVIDQLPPGRGSITTRVFNEKERFRVYRILREEIAKGKQAYIVYPLVEESERLDL
                     KDATQMAKNLQRDIFPEFKIGLIHGRLKSEEKEAIMADFKARRIHILVSTIVIEVGID
                     VPNASVMVIEHAERFGLSQLHQLRGRVGRGKDPSQCLLIAQFPKSEEAERRLRVMEQT
                     TDGFKIAEEDLAIRGPGELMGTLQSGLPDFRVANFARDIPLLNEARKEAFGIISRDPI
                     LSLPEHYFLKETLKERWKGRLELATIG"
     gene            8687..8977
                     /locus_tag="VEL68_01415"
     CDS             8687..8977
                     /locus_tag="VEL68_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006624758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXZ34656.1"
                     /translation="MRINEVLEKRRMEILGIAGRYGARNIRLFGSIARGTATEASDVD
                     FLLEMEGGRSLLDLVAIKQELEDLLGRQVHVVTEPSLSPYIRDDVLKEAVSL"
     gene            8974..9303
                     /locus_tag="VEL68_01420"
     CDS             8974..9303
                     /locus_tag="VEL68_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HXZ34657.1"
                     /translation="MKTDLVYLHHILDAILKTESYVSVGKAVFMQESHWQDAVIRQLE
                     IIGEATKRLSEELRSQHPEIPWRRIAGMRDVLIHDYLGVDIAAVWEVTQQELPELKRK
                     VGDILNG"
     gene            9601..10305
                     /locus_tag="VEL68_01425"
     CDS             9601..10305
                     /locus_tag="VEL68_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34658.1"
                     /translation="MPWEPFFSLFIRRKEAAETRKIILLGLMVLFLSPAVCAQEKVEA
                     PVWNVGDKWTFTGNGSIEVAKADQSGYILKFSNETCIIETQTCNAILFEKNTRNRINA
                     VEGDKRKKYIMGFRKILDFPFSAGKQWKDAYSAKELIGGGALEHLIDYSENFRILGWE
                     DIEVKAGKFKVFKLEYKAIANSPHPWGPKDQEIKHYYWYSPDAKYFTKCQYDKVWMKD
                     KKEIFNWELTSLQLKK"
     gene            complement(10592..10960)
                     /locus_tag="VEL68_01430"
     CDS             complement(10592..10960)
                     /locus_tag="VEL68_01430"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HXZ34659.1"
                     /translation="MGRPALIHRLDQSIQEDNNHYRSGHEQKREAVLQEDAKMKIPKF
                     KNIKEEREFWAAHDSTDYHDDFEVTKDVVFVRPKKEVLSLRLEPKIIQKLKDRAEKEG
                     LPPTTYARMLIIKGMREGAA"
     gene            complement(11027..>11904)
                     /locus_tag="VEL68_01435"
     CDS             complement(11027..>11904)
                     /locus_tag="VEL68_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXZ34660.1"
                     /translation="IPERILPAVKPSSQLYGYTSPAVFFGGEVPLSGIIGDQQSALFG
                     QTCFRQGQAKNTYGTGCFLLMNTGEKPVGSQNNLLTTVAWGTGKKVEYALEGSVFIAG
                     AVVQWLRDGLRIISRAADSEPMAASLSSNEGVYFVPAFVGLGAPYWDMYARGTILGVT
                     RGTTQAHIARAGLESIAYQTRDVLECMEKDSGIRLKELRVDGGAAANSFLMQFQADIL
                     GVPVVIQETTETTALGAAYLAGLAVGFWKDTQQVARNFKVRKKFTPRMPFKQRESLYR
                     KWKRAVERARRWEEE"
BASE COUNT         2786 a         3326 c         3202 g         2590 t
ORIGIN      
        1 gtacgaaatc ggggacgaga tgttccgttt aaaagaccgg aaccagcggg atatggttct
       61 ggggatgacc cacgaggaaa ttatcgcttg gctagccgcg cgggaggtcc gttcctatcg
      121 tgatctcccc cagatttggt accaaatcca gaccaagctg cgggacgaag cacgccctaa
      181 aagtggggtt ctgcgcaccc gggaattcct gatgaaagac tcctacaacc tggacttgga
      241 tgccgccggg ctggaccaga gttaccaact tcactatgac gcctacgtca aggtcttccg
      301 gcgttgcgga gtgaagttct atgtagtgga gagcgaccca ggcatgatgg gcggagcagg
      361 ggcccacgag ttcatggccc ccagcgaggc gggcgaggac gaagtggccc gctgcgagaa
      421 ctgcggctac gccgccaatg tggaattggc caaatccacc tctttccccc ctgatttccc
      481 gtcctggaag ttcgaagaag tggccacccc ggattcccgg acgatcgaag aagtctgctc
      541 ctacctgggg atttctcccc aactcaccat caagtccttg ctactaatgg ccaacgataa
      601 acccgtaatg gccctggtcc ggggtgacca gcagctccac gagaaaaaat tccggtccct
      661 ggtgggggaa ttcaggcccg cccaccggga agaggtcaag caatttaccg gggtggaagc
      721 agggtttctc ggcccggcca ggctttctcc caaacagccc atttccatga tcgccgatac
      781 ctccctgaag gaaggggttt tcgtcgccgg cgccaaccgg gagggttatc atctgcgggg
      841 ggtcgttccg ggagagcatt tctccgcccg gttcgccgac attcacctag ccatgtcggg
      901 ggatgcctgc ccccaatgcc aggcgcctct caaggtggaa aagaccatcg agatcggtaa
      961 tatcttcaag ctggggacca agtactccat cccgctcaag gccctgtacc tggaccggca
     1021 ggggcaggaa aagccgatcg tcatggggag ctacggaatc gggccggcga ggatcattgc
     1081 cgctgccgtg gagcagaacc acgactcgaa cggaatcatc ttcccgcttt ccataacccc
     1141 cttcgaggtt catctcctgc ccgtcaattt gaagcaggga acgatcgggg cagaagcgga
     1201 aaaactctat cagggattat ccacggccaa ggtcgaaacg ctcttcgacg accgggagga
     1261 ggccccgggg gtaaagttca aggacgccga cctcctgggg gcccccctgc gtttgaccct
     1321 gagtgccaaa actctgaagg agaaagccgc ggaaatcaag gtccgcaggt ccggggaggt
     1381 ccatatgatc cctctggatc gggctgtttc ctgggtcagg gattggatcg acgaccaaag
     1441 gaagaaatag ttcccggttc ccggtttcct gttgccggtt gaaagtcaaa gacgggaacg
     1501 gatcctattg atggttcaac accgggaacc ggcaacgggc aactgctttg tcatgatacg
     1561 tctcaacgac atactggaaa aagtcagccg caacttcccg ggggccaacc tcagcctgat
     1621 cgaaaaggcc tacgtctaca ccgccaaggt gcatcaggga caagtccggc tttcgggaga
     1681 accttacctc atccaccctc tggcagtggc cgctatcctg gccgatatga agatggacat
     1741 tgctacggtg gccgccgggc tgctccatga cacggtggag gacaccttcg ccaccctgga
     1801 ggagatcaaa gacctcttcg ggccagagat cgccgccctg gtggacggcc tcagcaaaat
     1861 cagcaaaatg accctggcca cccatgagga gcaacagtca gagaattttc gcaagatgct
     1921 cctggccatg tccagggaca ttcgtatcgt cctcatcaag ctggccgatc ggctccacaa
     1981 catgcggacc ttgcagcacc tgcccccgga agaccagaac aagatcgccc aggaaaccct
     2041 ggacatctat gctcccattg ccgggcggct ggggatcgac cgcatcaaga cggagctcga
     2101 agacctcacc ctcccttatc tccacccgga ggagtaccgc cgtctgatgg cggccatcgc
     2161 ctggaaaaag gaagaaaggg aaaaatacat ccaggaggtc agttccatca tctcggagaa
     2221 actggcctct tacgggctgg tgggaaaggt cagcggccgt ttaaagcact tctacagcat
     2281 ctaccgaaaa atgaaagccc agaacctgga attcgagcag gtctacgaca tcatcgcctt
     2341 ccgcatgatc ctctcctcag tcaaagattg ctacgaagcc ctgggaatca tccattcctt
     2401 gtggaaaccg atcccgggcc gcttcaaaga ttacatcgcc atgcccaagg ccaacgggta
     2461 tcaatcactc cagaccaccg tgatcggacc ctatggcgag cgggtggaaa tccagatccg
     2521 gaccgaagag atgaaccgaa ccgccgagga gggtatcgcc gcccactggc agtataagga
     2581 aggaaaggcc tttgacccca aagacacccg gcagattgcc tgggtccgcc agcttctgga
     2641 gtggcaccag gatttgcgcg atccccggga gttcatcgag acggtcaaga tcgacctctt
     2701 ccccgatgaa gtgtacgtct tcaccccccg gggggatgtg aagcagtttc ccatcggagc
     2761 cacacccgtc gacttcgcct acagcattca taccgagatc ggtcatcagt gcgtgggggc
     2821 gaaggtgaac ggcaagatcg ttcccctgcg ctaccagctc cagaacggtg acagggtgga
     2881 aatcgtcacc tcccccgggc accgccccag caaggactgg ctcaagcatg tgaagacctc
     2941 tcgggcacgg accaaaatcc gccagtgggt caaagccgaa gcccgggagc gatcggtgga
     3001 cctggggcgg gagatctgcg agcgggagtt caagaaatac catctggact tcccaaaatt
     3061 tctaaagtcc gaggacctga aaaaggtcat gggcgacttt tccttccagt cggtcgatga
     3121 cctgatggct gaaatcggtt acggcaacat ctcctgcaag caggtcgtgg gaaagcttct
     3181 gccgccggac aagttagaaa aggggaaggt cgaggaatcc cgtctaaagc gcctggcgga
     3241 caagatccgc cgcgaaccct cggggatcca gatccgggga attagggaca tgatggtccg
     3301 cttcggcaaa tgctgcaatc ccctccccgg agaccggatc accgggtaca tcacccgggg
     3361 gcggggggtt accgtacaca ccgcagattg ccccaatgtt ctggggggcg acccggatcg
     3421 cctgattcag gtttcctgga acctgcaaga aaaagcggtt cacgccgtac gggtacgggt
     3481 gacctgtaac gacaagaaag ggctgctggc tgaaatcggc gcggctctgg cttcgtcgga
     3541 gatcaacatt ctccaggccc atgtggtcac taccgaggac cggaaggcca tctgcaactt
     3601 tgaactggag gtgagggacc tcaagcatct ccaggcggct ttccgtaccc tgaccaagtt
     3661 gaaagatgtt ttgaaggtgg aacgaatgcg gggggcgact tcaggggaaa gggaagaacc
     3721 gggggcggaa ccctaaccca gttccgggtt ttaggtttct ggttttgtct tattgaactc
     3781 gagactcgaa acattcctca gatggctttg acgacccggc cggaacgaag gcaacgggta
     3841 cagacccgaa tgcggcgcga agtcccattt tggatggcgt gaaccctttg caggttcggg
     3901 taccaccggc gtttggattt aatgttggaa tggcttactt tatgccctac cttaggactc
     3961 tttccgcaga cctcacaagc ccgggccata gtgctcctcc caaaaagaaa aacaaatagc
     4021 aggggcaacc ccctgctttg gtaatttata gcatgttgct ctagttctgg caagaaatat
     4081 ctttactttg cgatgctcag gaagggaaaa agtcttctcc cccttatggg tccaaatggc
     4141 tcggcggggg gttgatcata aaggaatcgg ggacctgagg gactcctttg gccaggaccc
     4201 ggcgcccttg aatttccagc tgccctctag catacatctg gatggcctga gaataaattt
     4261 tgtgttcccg cttcaagatc cgggcattca gggtatccgc tgtatcatca tcgtagactg
     4321 gaactacgcc ttgaatgatg atcggccccg tatccgtgcc ttcatccaca aagtgcacgg
     4381 tacagccggc aaatttgacc ccgtagtcga tttgggtctg ccagacatgg gttcccggga
     4441 aggcaggaag cagagccgga tggatgttca tcaccctcat aggaaaggcc cggagaagaa
     4501 cggaagtaat gattcgcatg aagcccgcca ggatcaccag ctccacctcg tgggccttta
     4561 aggcctccac caccttctgg tcatacgctt ccctcttgag tcccttgtgc agaatgacca
     4621 gggagggaat tccgtgttta ttggcccgct ccaaggcata gacttccgga aggttgctga
     4681 ttacgatttt aatcctcgcg tccacccggc cggcctcgat gttgtcgatg atggcctgaa
     4741 ggttggagcc gctccccgaa accaggaccc cgaggttaac catattcctc cttacaggta
     4801 ggtgatggga ctatcctttg gatttacttt tttaacgatt tccccgatcg ggtatgcttt
     4861 ttctctccac cgcctcaacc gggcaatgac tccttccact acccttgggg gtactgcgag
     4921 aatcattcca atcccattgt tgaacgtgcg gagcatttcc tcctcggcca catttccggc
     4981 ctcctggaga actttgaaaa tgggatgaac gggccacgag cctttacgga tgactgcttt
     5041 gcatcccgcg ggaattaccc ggggcaagtt atccgtaatc cctccaccgg taatgtgggc
     5101 catcccgtgg acggggaagc tctggatcag gttcaacagc ggtttgacat aaatcttggt
     5161 ggggatcagc agctcttcgc cgattggcct ggaaaggcct ctcacccggc tgctgggctt
     5221 caatttcatc ctgtcaaaaa acaccttgcg ggccagggag tacccattgc tgtgcagccc
     5281 actggaggcg atccccacca gccggtctcc tgcgcggatg gccgagccat caataattcg
     5341 actcttctcc acaaccccga cggtgaaccc ggccaagtca tactccccct ctcgataaaa
     5401 ggaaggcatt tccgcggtct ccccgccgat caacgcgcag ccggcctgaa tgcaccctcg
     5461 ggccactcct ctaatgatcc gggtggaaac ccccagggcc agcttccctg tggccaaata
     5521 atccaggagg aaaagaggct cggctcccaa cacgactaca tcgttgacgc acatggccac
     5581 taggtctatg ccgacggtat cgtgcttccc ggtcgtgaaa gcgaccttca gtttcgtccc
     5641 cactccgtcg gtggaagaaa ccaggatggg attcttgttc tttttcgccc tcagagcaaa
     5701 gagccctcca aacccgccga tgtcggtcac cacctcgggt cgaaaggtct tccttactag
     5761 cggccggatc aaatctacga atttgttccc cgcgtcgatg tcgaccccgg cgcctcgata
     5821 ggtcattcgc ttgcccactt cccatcctcc cattcaaaat ttatacctat ttctaatgaa
     5881 aggccgacca aatgtcaata agcgaagttt tctcttacat tgcgaatttt ccctgctcgt
     5941 aggggtccgg gcgagtgcct ccttttcctc ggaccttctc tgaggggtgc tcttgccctt
     6001 tacttttctt cttgcccccc ggggaaaagt ttgttaccaa ttaattcaaa aggttgcccg
     6061 attttctttt ttctcatgat accatccgaa cttgaagaaa tccttgcggc tatcgagaaa
     6121 cctattcatt ttgcctcggg gcacgatttc gccaacctca agacggtgaa gggactcgag
     6181 ccctatttgg cccactggct ggacaaagcc acggctcttg ccctcagccc taggagggag
     6241 gaccttctcc agaggttgcg ggttcaggtc caggggtttg aaggattgga cctggatggc
     6301 aaaaaggccc gagtcgtaga ggtccagaag atcatccaag acctgaagtg tggggatgga
     6361 gtctccctcc cctgccagcc ttggccaacc caggcggaat tccaccagtc ccgaaaagag
     6421 ctacagacct ccatccagtt tatcaaaggc gtgggacccc gcctttcaga ggtcctcaag
     6481 aaaaaaaata tcctcaccgt ggaagacgcc ctctatttcc tcccccgcct ctaccaggac
     6541 cgcaggcaga tcaagactat ttcccagctt gcggtgggca aaatagaaac ggtcatgggt
     6601 actgtgctga aggccgacat gaccttttac ggccgccgga gaaccttcga attgaccgtt
     6661 ggcgatgaaa ccgggaccct catcgccaag tggttcaact tcaacccccg gttcatgaag
     6721 gggagattta aaaagggaat gcgcctgatc cttttcggcg aagtgaagtt ctaccagttc
     6781 cagaaggaaa tccatcaccc cgaactggaa atcttggagg aagagtctga atctcctgat
     6841 gggaatgcgg cccctccccc ggggaaagag gagtcactcc atttcggacg cgtcgtgccc
     6901 gtttattccg aaaccgaagg cctctatcag cggcagcgtc tcatccgtcg gatcatgaaa
     6961 aacgcggtgg acctcttcgc cggcaaggaa ttcagcggca tcccggaaga ggtctgcggg
     7021 cgccagagcc tgatcccctt gtccgaggct ttccgccgtg cgcattatcc cgacgccgat
     7081 gaagacctct ccctgctgaa cgagggaaag tcctcggccc atcgccgcct gatcttcgat
     7141 gaattcttct ttctagaact gggtctggct ttgcggcgta gcggcaccct catggaaaag
     7201 ggaatcccct ttccgatcag ccatcggcac actcgccggc tccgtgagat tcttcctttt
     7261 tccctcaccc cggcccagga acgggtgctg gcggagatcg agtccgacat gcgccggccc
     7321 catccgatga accgccttct ccagggagac gtgggcagcg gaaagaccat cgtggccctt
     7381 ctctccggcc tcatggccat cgagggggga tatcaggtcg cgatcatggc ccccacggaa
     7441 atactggccg aacagcacta tctcaacatt cggcccctgg ttgaaaaact cgggttccag
     7501 gcagctctcc ttaccagcag cctgaaaaaa tcggagaggg attcctttct tttacgaatc
     7561 cggaaggggg aaatccatgt cgtcatcgga acccacgccc tcatccagga gggagtggag
     7621 ttccagcacc tgggcctggc catcatcgac gagcagcaca agttcggggt tttgcagcgg
     7681 gccacgctca agaagaaagg gctgaacccg gatgtgctgg tgatgaccgc caccccgatt
     7741 ccccgaaccc tggccatgac gttatacggc gacctggagg tctcggtcat cgatcagctg
     7801 ccccccggga ggggaagcat caccacccgg gtctttaacg aaaaggagag attccgggtg
     7861 tatcgcattt tgcgggaaga aatcgctaag ggaaaacaag cctacatcgt ttaccccctg
     7921 gtggaggaat ccgagcggct ggacttgaag gatgccaccc agatggccaa gaatctgcag
     7981 cgggatattt tccccgaatt caaaatagga ctgatccacg gccggttgaa aagcgaggag
     8041 aaagaggcga tcatggccga cttcaaggcc cggcggattc acatcctggt ctcaaccatt
     8101 gtcatcgaag tgggcatcga tgttcctaac gcttcggtca tggtcatcga gcacgccgaa
     8161 cggttcggcc tttcccagct ccaccagctt cgggggcgcg taggaagagg gaaagaccct
     8221 tcccaatgtc tcctgattgc ccagttcccg aagagcgagg aggcggagcg aaggttgcgg
     8281 gtgatggagc agaccacgga tgggttcaaa atcgccgagg aagacctggc catccgcggg
     8341 ccgggtgaac tcatgggaac gctgcagtcc ggccttcccg acttccgggt ggccaacttc
     8401 gcccgggaca tcccgctcct gaacgaggcc cgtaaggagg ccttcggaat catctcccgc
     8461 gacccaattc tatccctacc ggagcattat tttctgaagg agacgctgaa agaaagatgg
     8521 aaaggaaggt tggagctggc gacgatcggc taagggaagc gccagaactt tgaaacgcgc
     8581 taaaacacct aatgctgtgg gggaagcaaa tcacaggtgg gtcggccgcc aaatattgcc
     8641 cgcaatatgg gaatcctgta ttatgtacct agaaggagcc aaaggtatga gaatcaatga
     8701 agtcctggaa aaaaggcgta tggaaatcct gggcatcgcg ggtcgttatg gcgcccggaa
     8761 tattcgattg tttggctcaa tcgcgagagg gacggccacg gaggcgagcg acgtggattt
     8821 cctcttagaa atggaggggg ggcgcagttt gctggatttg gtagccatca agcaggaatt
     8881 ggaagacctg cttggaaggc aagtgcatgt tgttacggag ccttccctca gcccctacat
     8941 tcgcgacgac gtcctcaagg aagccgtaag cctatgaaaa cagacctggt ctatctgcac
     9001 catattttag atgccattct gaaaacagaa tcctatgttt cggttggcaa ggcggtattc
     9061 atgcaggaga gccattggca agatgccgtc attcgacagt tagaaatcat cggggaggcc
     9121 accaaaagat tatcggaaga actccgttcc caacatcccg agattccttg gcgccggatc
     9181 gcaggaatgc gagatgttct gattcatgac tacctggggg tagatatcgc tgctgtctgg
     9241 gaggtcactc agcaggaatt gcccgagcta aaaaggaagg tcggagatat cctgaatgga
     9301 taagggagga gggtgaatcc catcatgggt gacgcatccc cacttcctgc taaatcgatt
     9361 ttgagaaagc ttatcaaatt cccgctcgcg cgagaattat aagcccctca gcaagaccaa
     9421 gcctatttcc aaaggcaatt cttggcttga ctacccatga ggatgagagt aatttgggca
     9481 taattgtagt accgttccgt tgcccagcaa agacaataat ctaaatttta accttccgga
     9541 gtaagtcatg ataaggatta ctagaaggtc tccacaagaa aggttacaag gggaaattct
     9601 ttgccctggg agcctttttt ttctttattc ataagaagga aggaggctgc ggaaacgagg
     9661 aaaataattc ttttaggatt aatggttcta tttctttctc cagcagtatg cgcgcaagag
     9721 aaggttgaag caccagtttg gaatgttgga gacaaatgga cttttaccgg taatgggagt
     9781 attgaggtcg ccaaagcaga ccagagcgga tatatcctga agttttcaaa tgaaacatgc
     9841 atcatagaaa cacagacctg taacgcaata ttattcgaga aaaacacccg caatagaatc
     9901 aatgcagtcg agggagacaa aaggaagaaa tacataatgg ggtttaggaa gattttagat
     9961 tttcctttta gtgctgggaa gcaatggaaa gatgcctatt cagcaaaaga actcattggg
    10021 ggaggggctt tggagcacct catagattat tctgaaaatt tcaggattct gggctgggaa
    10081 gatatagagg ttaaagccgg gaaatttaag gtttttaagt tggaatataa ggcaatagct
    10141 aattctccgc acccgtgggg gcctaaagat caagaaatca aacactatta ttggtattcg
    10201 cccgatgcca aatatttcac gaaatgccaa tatgataaag tttggatgaa ggataaaaag
    10261 gaaattttca actgggagtt gacttccttg caattgaaaa aataactttc tccgcttcct
    10321 attaaattct aaagcagcga ttgagtcgct gctgcacccc gcttttcatt cgggcgaccc
    10381 accgggtcgc ccctacattc tgtccttggg tttaccccgt tgatcttcgt gtcctgaatc
    10441 ttgggcccta ttcttcttcc caccccctcg ccctttccac ggccttcttc cacttccggt
    10501 acagagcctc tcgctgtttg aagggcatct ggggggtgaa ttttttccgg attttgaaat
    10561 tccgggcaaa ccagggttct cttgcgaaat tttacgctgc cccttctcgc attcctttga
    10621 tgattaacat ccgggcgtaa gttgtgggag gaaggccttc tttttctgct cggtctttca
    10681 gtttttggat aattttgggt tcgagcctta aagaaagaac ctctttcttc gggcgtacaa
    10741 aaactacatc tttggttacc tcgaaatcat cgtgataatc cgttgagtca tgagccgccc
    10801 aaaattccct ttcttctttt atattcttaa atttcggaat tttcatcttt gcatcctctt
    10861 gtagaactgc ctctcttttt tgctcatgtc ccgagcggta atgattattg tcctcctgta
    10921 tcgattgatc caaacgatga ataagagccg ggcgacccat cgggtcgccc ctacacccgg
    10981 tcctttgctc taatcccttc catcctcgtg cctcgaacct cgggccttac tcttcctccc
    11041 acctcctcgc cctttccacg gctctcttcc acttccggta aagagactct cgctgtttga
    11101 agggcatccg aggggtgaat tttttccgga ccttgaaatt ccgggccacc tgctgcgtgt
    11161 ctttccaaaa ccccacggcc aggccggcaa ggtaggcggc tcccagggca gttgtctcgg
    11221 tggtctcctg aatgacgacc ggaaccccca ggatatccgc ctggaactgc attaaaaacg
    11281 aattagccgc ggctccacca tccacccgca gctccttcag ccggatgccg gagtccttct
    11341 ccatgcactc cagaacatcc cttgtttggt aggcgataga ctccaggcca gcccgggcaa
    11401 tatgggcttg agttgttccc cgggttaccc ccaggatcgt cccccgggca tacatgtccc
    11461 agtagggagc gcccaggccg acaaatgcgg ggacaaaata aaccccctca ttcgaggaaa
    11521 ggctggccgc catgggctcg ctatccgcag cccgggagat gatccggagg ccatccctga
    11581 gccattggac caccgccccg gcaatgaaga cgcttccctc cagggcatac tctaccttct
    11641 ttccggtccc ccacgcgacg gtcgtcagga gattgttttg ggagcccacc ggtttctcac
    11701 ctgtgttcat gaggaggaag caacccgttc cgtaggtatt tttggcctgc ccctggcgga
    11761 agcacgtctg tccgaagaga gcactctgct gatccccgat gattccggac agggggactt
    11821 cccccccgaa gaagaccgcc ggggaggtat acccgtagag ctggctggag ggcttgaccg
    11881 caggaaggat tctttccgga atat
//
LOCUS       DASPRR010000037         8730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2107312, whole genome shotgun sequence.
ACCESSION   DASPRR010000037 DASPRR010000000
VERSION     DASPRR010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107312"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VEL68_01440"
     CDS             complement(<1..239)
                     /locus_tag="VEL68_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34661.1"
                     /translation="MKGDRWWFLAILGMALSFSFSPDAFAQKTYPIGAFWPMTGPQAY
                     YGRVMSRGALTGIDQINESGGVEGYQFKLIITDYK"
     gene            complement(363..1541)
                     /locus_tag="VEL68_01445"
     CDS             complement(363..1541)
                     /locus_tag="VEL68_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HXZ34662.1"
                     /translation="MYDVLLRHGEVIDPAQEIHRIASVAIQNGKIAEIADDISEAKAK
                     KVIPAEGKIITPGLIDIHCHPGAGLVWIGVSADEIGLDTGVTLLGDAGTSGWANFQAF
                     RSLVVEKAKTDILCFLNLASTGLATLPEIWSEKDIDIPRLVDVVASNREVIRGIKLRC
                     VQALEESMGIKAVKMAKKIATDHRLPLMIHIGETRKRTEDHKMDDFSRAAVSLMERGD
                     ILSHYLTWEPGGLILKDGTIYPELIDAQKRGVLLDSCHGLNHFSFAIARHALDMGMIP
                     DVVSTDLATVSQPAAQSLVVAMSKFLNLGVSLDRVIKMATIHPARALGEDGRRGSLKA
                     GMPADITLLKLERGNFIFSDGTGGERIIGELLLEPTGVFKKGRFYPAFSGYHIPPIYA
                     "
     gene            complement(1686..2396)
                     /locus_tag="VEL68_01450"
     CDS             complement(1686..2396)
                     /locus_tag="VEL68_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34663.1"
                     /translation="MLQISGLRVNYAKVQALHGVDIAVRKGEIAAIIGANGAGKTTTL
                     NAVLRLVPWAAGEITFDGRSLKHLRSHQMIRVGLGTVLEGRQLFSDQSVEDNLLLGAY
                     GLGRRSLAQVKENIERELKRFPILASRRRQLAGTLSGGEQQMLALARAMVADPKMLLL
                     DEPTMGLAPVIIKEVVKTISDLRARGLTILWVEQMAYVALQIADRVYILENGRIVREG
                     KGQELLNDSHVIEAYLGA"
     gene            complement(2400..4214)
                     /locus_tag="VEL68_01455"
     CDS             complement(2400..4214)
                     /locus_tag="VEL68_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXZ34664.1"
                     /translation="MKKSEGRFPSPVLADYSSSRFIGLAVAAVIIYLLPKIFADPFFI
                     LILQTIGFTYMAVLGLDFLVGFTGQKSLGHQGFFAIGGYTTVLLTTRLGWDFWWAFPV
                     AIVFSAIAGLIMAVSAFRAKGPYIAMVTIAFGFVIEVIANRWVALTNGPMGIFGITKP
                     HWFGGPLNPTGYFYLIAYMALALTMTTLNMLTSRVGRTLVAMRESEVAAESLGISVYA
                     WKTMAFVVSGIFAGIGGVFFAHQSSFINSESFRFSQAIMFLAAAILGGSGTVLGPLVG
                     TVVLVLLPHIFAGLYKYYLFIYGLILLLSIIFFNKGIVGSLAGMGGFGWLTPKKSRLD
                     VPAEKLAFAVSAPNDRPLLAVSGLSRYFGGIKAVDGIDYQLYPGYIYGLIGPNGSGKS
                     TLVNVLSGVYQPTAGRVEFKGETISGAKSHQMAVKGIARTFQTIRLFRHMTVLENIMV
                     GFHIHMRRGFWGHMLRTRATVKEEEHFMGLALALLARLGMTDKADTLVENLPYGHQRL
                     VEIGRALGTFPALLFLDEPAAGMNAHEIRELREIILGLKTAGIPVIVVIEHHMDLVMN
                     LCESITVLDFGQKICQGSPSEVQSDPKVCTAYLGQAEV"
     gene            complement(4201..5085)
                     /locus_tag="VEL68_01460"
     CDS             complement(4201..5085)
                     /locus_tag="VEL68_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003385797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34665.1"
                     /translation="MFEQIWQAIISGLAQGSVYALIAQGFFLTWVTTKSLNFGQGEFL
                     MMGAMFGLSLFGYLGVTGVNPFVAGLLCIAATIVLGLALERVAVRPVIHLASFSWVLS
                     TVAVSIMLKNVAVVGWGPAETKFPAYISEKMIHISGAGIAPQELFCLVASVTTMLALF
                     LFLKRARLGKAMMAVANNVSAASVVGINPTFIMALAYALSSGLAGLGGILIAPITFAF
                     AEMGALITIKAFAAALAGGLDNPLGILLGGLIIGLIEQGATMISSDIRDLSIFAFVLL
                     LLALRPTGLLGVKAIEKV"
     gene            complement(5149..6300)
                     /locus_tag="VEL68_01465"
     CDS             complement(5149..6300)
                     /locus_tag="VEL68_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34666.1"
                     /translation="MSNMKLTAVMGTIIFCLVVSNPLFAAELNLGYAGAVVGGHLGDY
                     GQGNKQGIELAVKRYNEKGGYKGQKVNLIIYDTEGKPPKAVENITRLITRDKCFALLG
                     ATASGETLAIIDIAQENEVPLLVATSTAAEVIQRHSSKPKMYIFRVSLMDTYQISLLL
                     DYIQAKGFKKLTLLNSTSGWGETAREDTLKQLAARNLKLAVPPERCNDTDTDMTSQLS
                     KFKAVNADFLLGMVYSPAAVQIVKSMQKINFKLPMASTWGLIAPNYLALGGKEIVEGT
                     ITVASFAPDANDKAKAFHDVVVKEYGKDFFPVCTAQGYDATNLILRALDKVGPEPKKI
                     RDAIESLDDFDGVTSIPKKPFTPKSHEAYGPKDGYLVIWRDGVPVRLQY"
     gene            6885..7976
                     /locus_tag="VEL68_01470"
     CDS             6885..7976
                     /locus_tag="VEL68_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HXZ34667.1"
                     /translation="MSAYKIAVLPGDGIGPEVMKETLKVLKAVEGAFPELRFQWKECP
                     TGARCYLEKGSDLPAETMKACKETDAILFGSAGLPDVRYADGTEIAPQLTLRFTLDLY
                     AGIRPIKKYAGIRPVLAGDPPIDYVIFRENTEGLYASRGGGVRVGNELAVDNMVITRK
                     GTERIVKYAFRLAQKRKGAPADGKKRVTCVDKANVLRSMAFFREVFDRVASSFPGIDP
                     EHAYVDAMTLYMVQKPQRFDVVVLENMFGDILSDLAAGTIGGMGLAPSADVGDTYGLF
                     QPSHGSAPDIAGKGIANPVAQILSAAMMLDWLGERKGDPEAHRGAAAIESAMADVLRD
                     PKYHTPDLGGKAVTAMIGDAVAARIAGAN"
     gene            8188..>8730
                     /gene="dctP"
                     /locus_tag="VEL68_01475"
     CDS             8188..>8730
                     /gene="dctP"
                     /locus_tag="VEL68_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ34668.1"
                     /translation="MKRKSFVSKMLWVTVVVFAFGLGTAEAQTVLKFSHTDQQQGARQ
                     GAANLFAQKVAQYTNNRYKVQVYCCSQLGNDPKNIEQLALGGIDFTVSSTGSYAPHVD
                     SLNLTMLPFLVETYAQGWKLYDDSKWLKAQFDKAPQKGFRFLATWEAGFRCMTTKEPL
                     RTPEDAKGKKLRTFPNEMMRW"
BASE COUNT         2059 a         2431 c         2414 g         1826 t
ORIGIN      
        1 ttcttataat cagtgatgat caatttgaat tggtagcctt ccacccctcc tgattcattg
       61 atctggtcga tcccggtcag cgccccgcga ctcattaccc gtccgtaata tgcctgcggg
      121 ccggtcatcg gccagaaggc ccctatcgga tatgtctttt gcgcaaaggc atcaggcgaa
      181 aacgaaaagc tcaaagccat gcccaaaatt gccaagaacc accaacgatc ccctttcatc
      241 tttcaccctc ctttcaagtc aggatttttc cgaaccatta ggagaaagcc acgttgaaac
      301 atacatgact ctctcccacc tcctttcgga ttcttttcta gaaacagatg tttcctcgaa
      361 tcctacgcat aaatcggagg gatgtggtat cctgaaaagg ccggatagaa cctgcctttt
      421 ttgaataccc cggtaggttc caacaagagt tcccctataa tcctctctcc acccgtccca
      481 tcactgaaga taaaattccc cctctccaat ttcaaaaggg tgatgtccgc aggcatcccc
      541 gccttcaggc tgcctcgcct tccatcttcg ccaagcgccc tggcagggtg gatggtcgcc
      601 atttttataa cccggtcgag gctgactcct aaattaagga atttggacat ggcaaccacg
      661 agcgactgcg ccgccggctg gctcaccgtg gcaaggtccg tgctcaccac atcaggaatc
      721 atccccatgt ccagtgcatg gcgggcgatg gcaaagctga agtggttgag accgtggcag
      781 gaatccaaca acactcccct tttttgggcg tcgatgagtt ctggatagat ggttccatcc
      841 ttcaagatca gaccgcccgg ttcccaggtc agataatggc tgagaatgtc tccccgttcc
      901 ataagggaaa cagcggcccg ggaaaaatcg tccattttat ggtcttcggt acgctttcgg
      961 gtttcgccaa tgtggatcat gaggggaagc ctgtggtcgg tggcaatctt tttcgccatc
     1021 ttcacggctt ttatccccat gctttcctcc agcgcctgaa cgcagcgaag ctttatcccc
     1081 cggatgactt cccgattgga agcgaccaca tccaccaagc gggggatgtc gatatccttt
     1141 tccgaccaga tttccggcaa agttgccagg cctgtgctcg ccaggtttaa aaaacagagg
     1201 atatcggtct tggccttctc cacaacaagc gagcgaaaag cctggaaatt ggcccagcct
     1261 gaggtacccg catcgcccag cagggtaacc cccgtgtcca gccctatctc atctgcggaa
     1321 acgccaatcc agaccaagcc cgccccggga tggcaatgaa tatcgatcag acccggcgta
     1381 atgatctttc cctcggcagg gatgactttc tttgctttcg cttccgaaat gtcatcggct
     1441 atttcggcaa ttttgccgtt ctgaatcgcc accgaggcga tcctgtgaat ttcttgagct
     1501 gggtctatga cttctccgtg tctcaaaagg acatcataca taacagttct ctctttcgtt
     1561 tgacggattg aatgcagggg aagaattcat tctagataga ccttgtcccg aacctttccc
     1621 tttagctctg cattgtcgcc gaataaattc ttgaaatccg caccgaggta tttttccgcc
     1681 tcttctcacg cccctaaata agcctcgatg acgtgagaat cgttgaggag ttcttggcct
     1741 ttgccttccc ggacgatcct cccattttcg aggatataga cgcggtcagc gatttgcagg
     1801 gcgacgtaag ccatttgttc aacccacagg atggtcaagc cccgggctcg aaggtccgag
     1861 atcgtcttca ctacctcttt gataatcacc ggcgctaacc ccatggtcgg ctcatccagg
     1921 aggagcatct tggggtcagc gaccatcgcc ctggctaaag cgagcatctg ttgctcgccg
     1981 ccgcttaacg ttccggccag ctgtcgacgt cgggatgcaa gtattgggaa acgtttcagc
     2041 tcccgctcga tgttctcctt cacttgagcc agactccgcc gccccaggcc gtaggcgccc
     2101 agaagaaggt tgtcctccac gctctggtcg ctaaagagtt gccttccttc cagtaccgta
     2161 cccaagccca cgcgaatcat ttggtggcta cggagatgtt tcagcgagcg accatcgaag
     2221 gtgatttccc ccgcggccca gggtacaaga cgcagcacgg cgttcagggt cgttgtcttg
     2281 cctgccccat tggcgccgat gatggcggcg atttcgccct tgcgaacagc gatatccacg
     2341 ccatgcaacg cctgcacttt cgcgtagttg acgcgaaggc cgctaatttg aagcatcggt
     2401 tacacctctg cctgtcccag gtaggccgtg cataccttgg ggtcactctg gacctcagag
     2461 ggagaaccct ggcagatttt ctggccaaag tcgagtacgg ttatggattc gcaaaggttc
     2521 ataaccaggt ccatatggtg ctcgataacc acgataactg ggataccggc tgttttcaga
     2581 cccagaatga tttctcgaag ctcccggatt tcatgggcat tcatgccggc agccggctcg
     2641 tccaggaata ataaagccgg gaaagtgccc aaggcgcggc cgatttccac caaacgctgg
     2701 tggccatagg gcaaattttc cacgagggtg tcagccttat cggtcatccc caggcgagcc
     2761 aggagcgcca aggcgagacc catgaaatgt tcttcctctt tcaccgtggc ccgggtgcga
     2821 agcatgtgtc cccaaaaccc ccgccgcatg tggatatgga aaccgaccat gatattttcc
     2881 aagacggtca tatgtctgaa aagtcggatt gtctggaagg tccgggcgat ccccttcacc
     2941 gccatttgat gggacttcgc tccggaaatg gtttcgcctt taaactcgac gcggccggct
     3001 gtaggctggt acacgccact aaggacgtta accagggtag acttacccga gccgttggga
     3061 ccgatgaggc cgtagatata gccggggtaa agctggtaat cgatgccatc cacggcttta
     3121 atgcccccga aatagcggct gaggccgctc accgccagca gggggcgatc gttgggagca
     3181 ctgaccgcaa aggcgagctt ctcggcgggt acgtccaacc tgcttttctt cggagtaagc
     3241 cagccaaatc ctcccattcc ggccagggaa ccgacgatcc ccttgttgaa aaaaataatg
     3301 ctcaacagga ggatcaagcc atagatgaaa aggtaatact tgtacaatcc ggcgaagata
     3361 tggggcagaa ggacgaggac caccgtcccc accaaaggtc ccagaacggt accgctgccg
     3421 ccaaggatcg cggcggccaa aaacatgatc gcctggctga agcgaaagct ttcggagttg
     3481 atgaagctgc tttggtgggc aaagaagacc ccgccgattc ctgcaaaaat tccgctgacc
     3541 acgaaagcca tcgtcttcca agcgtaaacg ctgatgccca ggctttcagc ggcaacttcg
     3601 ctctcccgca tggccacgag ggtacgtccc actcgcgaag tcaacatgtt gagggtggtc
     3661 atcgtcaagg ccaacgccat gtaagcaatc agataaaaat acccggtggg atttaagggg
     3721 ccgccgaacc aatgcggctt ggtgatgccg aaaatgccca tcggcccgtt ggtcagggcg
     3781 acccagcggt tggcgatgac ctcgatcacg aatccgaaag ctatcgtaac catggcgatg
     3841 tatgggccct tggcccggaa agcggagacg gccatgatca aacccgcaat ggccgaaaaa
     3901 acaatggcca ctggaaaagc ccaccagaaa tcccagccaa gcctggtggt caaaagaact
     3961 gtggtataac cgccgatcgc gaaaaaaccc tggtgtccca atgacttttg tccggtaaaa
     4021 ccgactaaaa aatcgagccc gagtacggcc atataggtga agccgatggt ctggagaatc
     4081 aaaatgaaga acgggtcggc gaaaatcttg ggcaagagat aaataatgac cgcagccacc
     4141 gccaagccta tgaaacggga ggaagaatag tccgcaagaa cgggagaggg aaagcgccct
     4201 tcagactttt tcaatggctt tcactcccag aagcccagtt ggccgaaggg ccaagagaag
     4261 gaggacgaag gcaaagatgg agaggtcgcg gatatcggaa ctgatcatcg ttgcgccctg
     4321 ttcgatgagg ccgatgatca atcctcccag aaggatgccc aggggattgt ccaggccacc
     4381 cgccaaggct gcggcaaagg ccttgatggt gatgagggcg cccatctccg cgaaggcaaa
     4441 agtaatcggg gcgatcagga tgccccccag cccggccaga cccgaagaga gggcataggc
     4501 cagggccatg ataaaagtag gattaatgcc gactaccgag gcagcggaca cattattcgc
     4561 cacggccatc atcgcctttc ccaagcgagc gcgcttcagg aacaagaaca aagccagcat
     4621 ggtggtcacg ctcgccacca agcagaaaag ctcctgggga gcgataccgg cccccgagat
     4681 gtgaatcatc ttctcactga tgtaagcggg aaatttcgtc tcggccggcc cccatcctac
     4741 aaccgccaca tttttgagca taatactcac ggcaaccgtg gagagcaccc aactaaagga
     4801 agcaaggtga atgaccggac ggacagccac tcgctccagg gccaagccca aaacgatcgt
     4861 ggcggcgatg cacaggagac ccgccacaaa ggggttcact cccgttaccc ctaggtagcc
     4921 gaataaactc aggccaaaca tggcccccat cattaaaaac tcgccctggc cgaaattcag
     4981 acttttggtg gtcacccagg taaggaaaaa gccctgggcg atgagggcat ataccgaccc
     5041 ctgcgccagg ccggagataa tcgcctgcca aatctgctcg aacatgacca aacctttagg
     5101 acatttcagg ctggggcgga atgaaattcg gtccgccgct gatgggactt aatactggag
     5161 acgaaccggc actccgtccc gccagattac cagataccca tcctttgggc catacgcttc
     5221 gtggctcttg ggggtgaagg gcttcttggg aattgaggtc accccgtcga aatcatccag
     5281 agattcgatg gcgtcgcgga ttttcttggg ttccggcccg accttgtcga gggcgcgaag
     5341 gatcaggttc gtggcgtcgt agccctgggc agtacaaacc gggaagaagt ctttgccgta
     5401 ctccttgaca acgacgtcat ggaaagcctt agccttatcg ttggcatccg gggcgaaaga
     5461 ggccaccgta attgtgcctt caacaatctc cttaccgccc agggctagat aattgggggc
     5521 gatcaaaccc caggtgctgg ccatgggcag tttgaaattg atcttctgca tggatttgac
     5581 gatctggacg gcagccggcg agtataccat tcccagcaag aaatcggcgt tgaccgcctt
     5641 aaacttcgat aattgggagg tcatgtcggt atcggtgtcg ttacaacgtt ccggcggcac
     5701 ggccagcttg aggttacgcg cggccagctg tttcagggta tcctcgcggg ccgtctcgcc
     5761 ccagccgctg gtggagttga gcagggtcaa ctttttgaag cctttggcct ggatatagtc
     5821 gaggagcaag gaaatctgat aggtatccat cagggaaacg cggaaaatat acattttggg
     5881 cttgctgctg tgccgctgga tgacctcggc tgcggtcgac gtcgcaacga gcaaggggac
     5941 ttcgttttcc tgggcgatat caatgatcgc cagggtttcg ccggacgcgg ttgcgcccag
     6001 aagggcgaaa cacttatcgc gggtgatcaa acgggtgatg ttttccacgg ccttgggcgg
     6061 ctttccttcg gtgtcgtaaa taatcaggtt gactttctgg cccttataac cgcccttttc
     6121 gttgtaacgt ttaacggcca gctcaatccc ctgcttgttc ccttggccgt agtcgcccag
     6181 gtgtccgccg accacagccc cggcgtatcc aaggttgagt tcggcggcga acaacgggtt
     6241 agagacaacc aggcaaaaaa tgatcgtacc catcacagcg gtcaatttca tattggacat
     6301 gacagacctc ctacgacatt ttctttaaag attgaattga taagaattcg cttgggggtg
     6361 aattgaatat accattgata agaactcgct tgggggtgaa ttgaatatac aaaaaggagg
     6421 gttgggtgtc aagggaattt tagaccttgc ccacccccag tttttcgtgc ttgttggggt
     6481 tcagggtgga ggaatttgga tcatcaggct cgccgggtgc ttatccttcc atgaaactcc
     6541 tccccatgtt ccgcagggag aggcctgaga ggagattcct ccaagcagaa aagagagcca
     6601 aagcattttt ccgtttcaat ccccccccag gacaggcccg atccaatgga agaaccaccg
     6661 cctcaggatg atgatccaaa tttctccgcc ctgaacccca tgaggtcccc cctcctttcc
     6721 agagatctta ttttcgaagg actctttttt aagaactggg ggtgggcaag ttccccccaa
     6781 cccttgacga agatttcgga aaaggctatt ttgaattcaa aattcgaaat gcaaatccat
     6841 ctggacctcc gcagaagggg cccggaaaag tggggaactt aaaaatgagc gcatacaaga
     6901 ttgcggtact tcccggcgac ggcatcggac ccgaggtgat gaaggagacc ctgaaagtat
     6961 tgaaagcagt ggaaggggcc tttccggagc tgcgtttcca gtggaaagaa tgcccgaccg
     7021 gtgcccgctg ctatctggag aagggcagcg atctgccggc cgagacgatg aaggcctgca
     7081 aagaaacgga cgcgatcctt tttgggtcgg cgggtctgcc ggacgtaagg tatgcggatg
     7141 gaacggagat cgccccccag ctgaccctcc gcttcaccct ggacctttac gccgggattc
     7201 ggcccattaa aaagtatgcg ggtatccgcc cggttctggc aggagacccg cccatcgatt
     7261 atgtcatctt ccgggagaat acggaggggc tctatgcctc gcgcggggga ggggtgcggg
     7321 tgggcaatga gctggcggtg gacaatatgg tcattacccg caaggggacg gagcggatcg
     7381 tgaaatacgc ctttcgcctg gcccagaaaa gaaagggggc tcccgcggat ggaaagaaac
     7441 gggtaacctg cgtggacaag gccaacgtcc tccggagcat ggcctttttc cgggaagtct
     7501 tcgaccgggt cgcctcttct ttcccgggga tcgaccccga gcatgcctac gtggacgcca
     7561 tgaccctgta tatggttcag aagccccaac ggttcgatgt ggtggtcctg gagaatatgt
     7621 ttggggatat cctttccgac ctggcggcgg ggactatcgg agggatggga ctggcccctt
     7681 ccgcggacgt gggcgacact tacgggctct tccagccttc ccacgggagt gcgccggata
     7741 ttgcgggcaa gggaatcgcc aacccggtgg cccagattct ctccgcggcc atgatgctgg
     7801 actggctggg agagagaaag ggggaccccg aagctcaccg aggggccgcg gccatcgaat
     7861 cggccatggc cgatgtcctg agggatccga agtatcacac ccccgatctg gggggaaaag
     7921 ctgtcaccgc gatgatcggc gacgcggtgg ctgcgaggat cgctggagcg aattgagttt
     7981 cgggttggta aggacgcgga gaaggccgag caggatgccc ggaaccattt tagacggtcc
     8041 agagagaagc tttcgaagga gatcaaaaag gaaggtaaga aaaatcgagg gaattcgggg
     8101 cagcccaagg gaggaagggg gcggaactgg atttcccttt cctcccctcc ggaataggtc
     8161 gctcaataaa tcaaacagga ggaaaagatg aagcgcaaat cgtttgtcag caagatgctc
     8221 tgggtcacgg tcgttgtttt cgccttcggc ctggggaccg ccgaagccca gacggtattg
     8281 aagttcagcc acacggacca gcagcagggc gcgcgtcaag gggccgccaa cctgttcgcg
     8341 cagaaagtgg cgcagtacac caataaccgc tacaaggtgc aggtgtactg ctgcagtcag
     8401 ctgggcaacg acccgaagaa catcgaacaa cttgccctgg ggggtatcga ctttaccgtg
     8461 tcgagtaccg gctcctatgc cccgcacgtc gactcgctca acctcaccat gctgccattc
     8521 ttggtcgaaa cgtatgcgca gggatggaag ctctacgacg actccaagtg gctgaaggcc
     8581 cagttcgata aggctcccca gaaaggtttc cgctttctgg ccacctggga ggcgggcttc
     8641 cgctgcatga ccaccaaaga gcccctgagg acccccgagg acgccaaagg gaagaagctc
     8701 cgaaccttcc ccaatgagat gatgcgctgg
//
LOCUS       DASPRR010000038         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1458995, whole genome shotgun sequence.
ACCESSION   DASPRR010000038 DASPRR010000000
VERSION     DASPRR010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458995"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VEL68_01480"
     CDS             <1..278
                     /locus_tag="VEL68_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34669.1"
                     /translation="MDICLIPETPNLGMITALPKLPFLGLKKLPGALSRTTSVLEIKS
                     SPPVSIHGDGDLLGQTPETFRVLPKALHIMAPPSKPRLLDLPFFKEG"
     gene            complement(456..1469)
                     /locus_tag="VEL68_01485"
     CDS             complement(456..1469)
                     /locus_tag="VEL68_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXZ34670.1"
                     /translation="MKAAVFKEKNLLVVEDVPDPQPRDDEVVMKVSYCAICGSDLHRY
                     SYGMMSPGTIMGHEYSGVIAEVGKKVKGFKVGDRATRSGGKIEPGRDLPNFPPRYSAK
                     ERGFLPLKPGAYAQYMATPEAMLMKVPDSLSDLEASLIEPLTVALHMVRISKVRLGDR
                     VIVLGAGPIGLFALQCAALAGAMKVYASDVNPARLKAASELGAHRVFNPSRVNLVQEV
                     VALTDGLGADLAFECAGAKPTLQQACEVVKMGGRVMVVSLAWEQVDCLPVEWVGREVE
                     VKACYGSLNSEWLISLALMAEKKIQTKPMITQIIGLGEIQEAFQELLKPHTSQVQVVV
                     RCN"
     gene            complement(1496..2278)
                     /locus_tag="VEL68_01490"
     CDS             complement(1496..2278)
                     /locus_tag="VEL68_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006065812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ34671.1"
                     /translation="MRLENQIAIVTGGGQGIGQQIALRLGQEGSVVVIADINEQGSRE
                     TAEMIVREGGKTPRVIPTDLVSEEQIRNLVQGALKIDNRIDILVNNSGIMGPVKPVEE
                     ITAEEWDATFAVNLRGMFLCCKHVVPPMKRQGKGSIVNISSITGKRPLTQRSPYAASK
                     MGVIGLTRTLAAELGEWKIRVNAVCPGAVAGPRLDRVFEGIMKFSGKSREQVMEERAK
                     ASPLRTFIDAKYIAAMVAFLCSEDAAMMTGQDINVSAGTVMY"
     gene            complement(2420..>3150)
                     /locus_tag="VEL68_01495"
     CDS             complement(2420..>3150)
                     /locus_tag="VEL68_01495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXZ34672.1"
                     /translation="ARKQPRREEGIPELEEILPLVGIRKAIADGMSLSVRTAAHVTTV
                     AEVDMTELVRLRSELAPQWKNEGINLTYVPFVIRAAARAIEKFPIINSQTLADRIIVK
                     KYVNFGVVVAIAEGLVTPVLKRVESRRVKEIARMMEDISTRARERKLTLEDMRGGTIT
                     LSNPGVFGAVLATPIIYQPQNSLLWMGRIAKTPVVREDVIVIRSMMYLCLSYDHRAID
                     GSIGAQFLQSVRKSLEAPATLLDG"
BASE COUNT          591 a          993 c          869 g          697 t
ORIGIN      
        1 tgatggatat ctgcctgatt cccgagactc caaacctggg gatgatcacc gctcttccca
       61 agctcccttt cctcgggctg aagaagctgc cgggggccct ctcccggacg acatcggtct
      121 tggaaattaa atcctccccc ccggtcagca ttcatggcga cggggacctc ctcggccaga
      181 cgccggaaac cttccgggta ctccccaagg ccctgcacat aatggccccc ccttcaaagc
      241 cccggctact ggacctgcct tttttcaagg aaggttaggt tgatttttcc gctttatcct
      301 ttactaggaa cccgggacgc tttcgccccc ggagcttcag gaccacggaa gagatcagaa
      361 ggaagaacat caggaagacg aagagagcgg agatggctcg ggtgaaaaaa atcgccgggc
      421 ttctcctcca aatgagagtt tgacttcacc catgactaat tgcatcggac cacgacctgg
      481 acctgggagg tatgcggctt cagtagctcc tggaaggctt cctggatttc ccccagcccg
      541 atgatttggg tgatcatggg tttggtttga atcttctttt ccgccatgag ggccagagaa
      601 atcagccact cgctgttcag gctgccgtag caggctttca cctccacttc cctgcccacc
      661 cattccaccg gcaggcaatc cacctgctcc cacgccaagg agaccaccat gactcttccg
      721 cccatcttca ccacctcgca ggcttgctga agggtgggct tcgctccggc gcattcgaag
      781 gccagatcgg ctcccaggcc gtcggtcagg gcaactacct cctggacaag gttgaccctg
      841 gaggggttaa agacccggtg ggctcccagc tccgaggcgg ccttcaaccg tgccgggttg
      901 acgtcggagg cgtagacctt catggcccct gctagggcgg cgcactgaag ggcgaaaagt
      961 ccgatggggc cggcccccag gacgatcact cgatctccca gccggacctt ggaaatacgg
     1021 accatgtgaa gggccacggt caagggctca atcaaggagg cttccaggtc ggaaagggaa
     1081 tcaggtacct tcatgagcat ggcttcgggg gtggccatat actgggcgta agccccaggt
     1141 ttcaagggaa ggaaacccct ctccttggcg ctgtagcgcg ggggaaaatt ggggaggtct
     1201 ctccccggtt cgatcttccc tcctgagcgg gttgctcgat ctcccacctt gaaccccttt
     1261 acctttttcc ccacctccgc gatgaccccc gaatattcgt gtcccataat ggttcccggg
     1321 gacatcatcc cgtaggagta ccggtgcagg tccgagccgc agatggcgca gtagcttacc
     1381 ttcattacca cttcatcgtc ccggggttgg gggtccggaa cgtcttccac caccagcagg
     1441 tttttctcct tgaaaaccgc ggctttcatc tcttctccct ttcacctctc ggatatcagt
     1501 acatcacggt ccctgcagat acgttgatat cctggccggt catcatggcc gcatcttccg
     1561 agcacaggaa ggcgaccata gcggcgatat atttggcgtc gatgaaggtc ctcagggggg
     1621 atgccttcgc cctctcttcc atcacctgct cccggctttt ccccgagaac ttcatgattc
     1681 cctcgaatac ccgatccagg cgggggccgg ccacggcccc ggggcagacc gcgttgaccc
     1741 tgattttcca ctcacccagc tccgcagcca gggtacgggt caggccgatt acccccatct
     1801 tggaggcagc ataggggctg cgctgagtga ggggcctctt gccggttatg gaggagatgt
     1861 tgacgatgct ccccttgccc tgcctcttca tgggcgggac cacgtgcttg cagcagagga
     1921 acatgccccg gaggttgacc gcgaaggtgg cgtcccactc ttccgcggtg atctcttcca
     1981 ctggtttcac cggccccatg atccctgagt tattgaccaa gatgtcgatc cggttgtcga
     2041 ttttcaaagc gccctgcacc agattcctga tctgttcttc ggagaccagg tcagtaggga
     2101 tcaccctggg ggttttccct ccttcccgaa caatcatttc ggcggtctcc ctggacccct
     2161 gctcgttgat gtcggcaatc acgacaaccg atccctcttg tcccagacga agagcaatct
     2221 gctgtccgat cccctgccct cccccggtga caatggcaat ttgattctcc aaccgcatct
     2281 gagctctcct tccagtcttt tcatatgaat ttagcttttt gaaatgccct tctaaaatcc
     2341 cccctgcgcc cccctttttc aaagggggga acggggggat ttctgcggat gccttgccca
     2401 aaagggcgat gtagagttct catccatcca gaagggtcgc cggggcttcc agggactttc
     2461 tcacggattg aaggaattgg gcgccgatgc tcccgtcgat cgcccgatgg tcgtaggaaa
     2521 ggcagaggta catcatggac cgaatgacga tgacgtcttc cctcaccacg ggggtcttgg
     2581 cgattcttcc catccacaga agggagttct gcggctgata gataatggga gtggccagca
     2641 cggcgccgaa gaccccgggg tttgaaaggg tgatcgtgcc cccccgcatg tcttccaggg
     2701 tcagcttccg ctcccgggcc cgggtggaaa tatcctccat catccgggcg atctccttga
     2761 cccttctgga ttccaccctt ttcagcacgg gtgtgaccag cccttcggca atggccacga
     2821 ccaccccgaa attgacgtac ttcttgacga tgatccggtc cgcgagggtc tgcgaattga
     2881 tgatggggaa tttttcgatt gcccgcgccg ccgcccggat cacgaagggg acgtaggtca
     2941 ggttgattcc ctcatttttc cattgcgggg ccagctcgga ccgcagccgc accagctcgg
     3001 tcatgtccac ttcagccacg gtggtgacgt gcgccgcggt gcggacgctg agggacatgc
     3061 cgtcggcgat ggccttgcgg attccgacca ggggaaggat ttcctccagc tcgggaatcc
     3121 cctcctcccg ccgtggctgc ttccgggcga
//
LOCUS       DASPRR010000039         8482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_493308, whole genome shotgun sequence.
ACCESSION   DASPRR010000039 DASPRR010000000
VERSION     DASPRR010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493308"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VEL68_01500"
     CDS             complement(<1..1447)
                     /locus_tag="VEL68_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34673.1"
                     /translation="MKNGFIKILLVVVLVCALATPAMAEKVLRWASQGDALTYDPHSA
                     NESPTNLAMQQVYETLAIRNPKYEIVPCLATSWKALDPKTWEFNLRKGVTFHDGTPFT
                     AEDVAFTITRAKGPTSEFRNYLTTVTGTKVIDDHTIHILTIGPDPVLINNLCYVFIMS
                     KKWCEKNGATEAQNFKAKEEKYTIRHENGTGPYILELREPDIKTTLVKNPKWWNAKTY
                     PYEIDKIIYTPIANPATRVAALLSGEVDFLLDPPFQDLDRLKKNPNLKVGQIAQNRSI
                     FFGLDQASNELRSSNIKGKNPFKDKRVRQAMNLAIDGYAIQKVIMRGLSQPANVITAP
                     MVYGYPKELDKRPPADVQKAKKLLAEAGYPNGFEVKLDCPNNRYNNDEKICQAVVGML
                     GKAGIKVNLDAQSKTLHMPKITNRTSDFYMLGWGVATVDSHYVFTYLTIKDGPWNGAG
                     YSDPRVEELTTAMSVEMDQTKRDAMITEVWKK"
     gene            complement(1890..3821)
                     /locus_tag="VEL68_01505"
     CDS             complement(1890..3821)
                     /locus_tag="VEL68_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ34674.1"
                     /translation="MGTDFSPLFEPLLIGRGVKKLTLKNRMVMAPMVTCLANSQGFVT
                     PRMVDYYVERAKGGIAAIVVEAMDIDDRMLFNRLGVFHDRFINELENLATSIKERGAA
                     AIAQINQTGIRGNTPGPDDLSADQMEELIVWFSQAAERVKKAEFDGVMIHGAHGYLIS
                     SFLSPLTNHRKDAYGGDRKARTHFAADVVRSVRRTVGDDFPIFFRMNGEDFLPGGFTV
                     EDARVAAPILEAAGADVISIGGGVGILAHDLSLGDNKSYGQMIMPMYIPRGCRVEIAA
                     EIKKGLNIPVSVAGRINDPFLARDIIAQKKVDLVDLGRQMIADPYFPQKIEQGQIQDI
                     GSCIACNYCHGKRMRAMKQVRCAINPWAGREAELREIKPAATSQKVMVVGGGIAGLQA
                     AQWLGKRGHRVFLYEKSDRLGGQIRLAAMPPHKEEIRQFLELSIRQIKKLKVKIHLNS
                     EVTPERLIQEHPQAVILATGGRPLRPPNIPLHDKMKCIYAWDVLSGKEKFLGEKTVVL
                     GGGFVGAEVADFIRSTGIAKEVEIVEMREAMALDLEPAFRQLLIERLQSLGVKMLSHF
                     RVREVTARQVIGEDVRTKDTKKIDADAVVIALGTESVEFPLEAVQKKGIRFFLIGDAH
                     TPRGIAEAVRDGYLAGISV"
     gene            4200..4937
                     /locus_tag="VEL68_01510"
     CDS             4200..4937
                     /locus_tag="VEL68_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34675.1"
                     /translation="MAFLEVKDLVKDFGGLRANDHVDLQVNQGEIVGLIGPNGAGKTT
                     LFNCIAGYYPPTSGAVTFQGENITGLPAYEICRRGIARTFQIVRVFKDMPVLDNVMVS
                     AFNRTKSAAVARRKALEVLDFCGMGSKKMMLAASLTIADKKRLEVAKALATAPALLML
                     DEAMAGLNQTETAEAVELVKKMEKAGLTILLVEHVMEVVMPISKRVVVLDYGRKIAED
                     APEKVIHKEEVIKAYLGDKYHATRRAP"
     gene            complement(4918..5070)
                     /locus_tag="VEL68_01515"
     CDS             complement(4918..5070)
                     /locus_tag="VEL68_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34676.1"
                     /translation="MRLTILALLLMVDFPAPLASTMGTIYSLNIEGYPVKSRYSGIGY
                     SRALDA"
     gene            5101..6378
                     /locus_tag="VEL68_01520"
     CDS             5101..6378
                     /locus_tag="VEL68_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34677.1"
                     /translation="MRRKTIVLAGIFIMALGILSPSVQAQPKEVLIGGIWPLTGPNAP
                     TGVECTQGATLAMEIINGKYDLNLPLAKTEGLPNLGGAKLKLVFGDHQNVPDKAMAEA
                     ERLITQEKVMALIGCYISGVTAPASQAAERRKVVFLDADAIAPTLIKRGFKYFFRVTQ
                     DDEIWFRDFMWTRFIPEVLKKKGGSIKNVAVLHENTLWGTDASKYGTEYAKKNGFNIV
                     TQIAYPARSTSLTAEVQKLKMVEKQTDMLLSACYTADGILLMKSFHEMDYNPPMIWND
                     GGYREPAFLEALGKKSEFILQRDEFSVDMGERKLLVKQVNDLFRKRFGVDMNTNSSRN
                     FMGVICLADAINRAGSTDSEAIRKALLATQIPPEQCICSWDGIQFDPNNGQNIKAVSL
                     MLQVHENKWRLVWPFEVAVKDLAYPIPKWSARP"
     gene            6446..7315
                     /locus_tag="VEL68_01525"
     CDS             6446..7315
                     /locus_tag="VEL68_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017972132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34678.1"
                     /translation="MLTTDIILQTFISGLLMGCIYALVAVGLCLIWGLMEIVNFAHGQ
                     FMMLAMFLSFWMYTLFGVDPLLSIPLGGLCLFIIGVATYHLIIKGILYTTFFAQIFAT
                     FGLGIFLHSAAQFLWTANYRSVQNPLLAGRVALGNIFIGFPQVAAAIMCILGFLFLYW
                     LINKTEIGLALQAASEDREAASLMGINSNRIFALGWGIGSACVGIAGVLLTNFFYIFP
                     EVGFTFALIAYVAVALGGFGSIVGSFLAGVIIGLVEAFAGLFIDPAFKYFSIYVLYMV
                     VVFFRPQGLWGRY"
     gene            7361..8296
                     /locus_tag="VEL68_01530"
     CDS             7361..8296
                     /locus_tag="VEL68_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34679.1"
                     /translation="MMKMIGFVGVGIFLLLFPFIFTLPFPQHIMIMIFVYATLAQAWN
                     IVGGYTGQCSLGHAVFFGCGAYTSSLLLIQLGLNPWLGMLAGAILAVGISFLVAIPCF
                     RLAGHYFAIATIAIGEICQIGMVNWKGGGGANGLYLPILEEGFLNLEFHNTKVPYYYI
                     ALGIAAITFAVVFLLERSFLGYYFRAIKGDLDASRAAGIHVARYKTVALAISAFFTSI
                     TGSFYAQYVLFIDPESVLPLMLSVLISLVAILGGVATVWGPFLGAAVLIPLSEFTRTY
                     LGGRGTAVDLMIYGLLIVLIARFQPTGLMGLLKKR"
BASE COUNT         1761 a         2338 c         2318 g         2065 t
ORIGIN      
        1 gtttcttcca gacctcggta atcatggcgt cgcgcttggt ctggtccatt tccacgctca
       61 tggccgtggt cagctcctcc acccggggat cgctgtagcc cgccccgttc cagggaccgt
      121 ccttgatcgt caggtaggtg aagacgtagt gggaatccac cgtggccacg ccccagccca
      181 gcatgtagaa atcggaggtt cggttggtga ttttgggcat atgaagggtc ttagactggg
      241 catccaggtt caccttgatg cccgccttgc ccagcatccc caccaccgcc tggcagatct
      301 tctcgtcgtt gttgtaacgg ttgttggggc aatccagttt gacttcaaat ccattgggat
      361 acccggcttc ggccaggagc ttcttcgcct tctgcacgtc ggcgggggga cgcttgtcca
      421 attccttggg gtacccatac accatgggcg cagtgatgac gttcgccggc tgggacaatc
      481 cgcgcatgat caccttttgg atggcatagc cgtcgatggc caggttcatg gcctggcgca
      541 cccgcttgtc tttgaaaggg ttcttgccct tgatgttgga ggagcgcagt tcgttgctgg
      601 cctggtcgag cccgaagaag atgctgcggt tctgggcgat ctggcccact ttcaaattcg
      661 ggttcttttt caaccggtcc agatcctgga aaggcgggtc caggaggaag tcgacctccc
      721 cggacagcag agccgctacc cgcgtggccg gattggcgat gggggtatag atgatcttgt
      781 cgatttcgta ggggtatgtc ttggcattcc accacttggg gttcttcacc agggtggtct
      841 tgatgtccgg ctcgcggagc tcgaggatgt aggggcctgt tccgttctcg tggcggatcg
      901 tatatttctc ttccttggcc ttgaaattct gcgcttccgt cgccccgttc ttctcgcacc
      961 acttcttcga catgatgaaa acgtaacaaa ggttgttgat caggaccggg tcggggccga
     1021 tggtcaggat gtggatggta tgatcatcga tcaccttggt gccggtgact gtggttaggt
     1081 aattccggaa ttccgaggtg gggcctttgg cccgggtgat ggtaaaggcc acgtcctcgg
     1141 cggtaaaagg ggttccgtcg tggaaggtaa ctcccttgcg caagttaaat tcccacgtct
     1201 tggggtcgag cgctttccag ctcgtggcca ggcagggaac gatctcgtac ttcgggttcc
     1261 gaatggccag agtctcatag acctgctgca ttgccaggtt cgtcggggac tcgttggcgg
     1321 aatggggatc gtaggtcaga gcgtctccct ggctggccca gcgaaggact ttctcggcca
     1381 tggccggagt ggccagggcg cagaccagaa ccactaccag taagatcttg ataaagccgt
     1441 ttttcatcgg ggtctccttt ctttttttca ccaaggatca cgggtttaac gggaccgctg
     1501 gtttccttga ttaaaatcgg aatgaacgat taaaaccggg atgagaagtg gatgctagcg
     1561 gtatcctcat tgatcgaaaa atgctttgca cgaatttccc ctcgaagagt tgctgaatcc
     1621 cttccggcat gagcctgggt cctctaagcg gtgcatcccc cccttcacct cccccaggac
     1681 tgggtcaacc cccggcatgc taaaagtagg cagtctcctg ggtttttccc cctttcggtc
     1741 ctaatcctgg ttgctattac cataaatcaa aaaaattgtc accctttata atcgccgttc
     1801 cccgaaggaa tgaaacctgg gcctggcgat attccccccg ggaaaaggag aagtcacttt
     1861 aaaaatccga tgacgccaaa aaagcgggtc tatacggaaa ttcccgccaa atatccgtcc
     1921 ctcactgctt cggcgattcc tcggggggtg tgagcatcgc cgatcaaaaa gaaccgaatt
     1981 cccttcttct ggacggcttc caggggaaat tccaccgatt ccgttcccag ggcaatcacc
     2041 accgcatccg catcaatctt ttttgtatcc ttggtgcgaa catcctctcc gataacttgt
     2101 cgggcggtca cttcccggac ccggaaatga gaaagcattt tcacaccgag gctctgcaac
     2161 ctttcgatta acagctgccg gaacgcaggc tccaaatcta aggccattgc ctccctcatc
     2221 tctacgatct ccacctcttt ggctattccc gtggagcgaa taaaatccgc tacttccgcg
     2281 cctacaaagc ctccgcccaa gacaacggtt ttttcgccca gaaatttttc ttttccagat
     2341 aagacatccc aagcatagat acacttcatc ttatcgtgga ggggtatatt cggaggtcga
     2401 aggggtcttc caccagtagc caggatgacc gcctgcgggt gttcctggat tagccgttca
     2461 ggggtgacct cagagttgag atgaattttg accttgagct ttttgatctg ccgaatcgat
     2521 agctccagga actgacggat ctcctctttg tggggaggca tggcggccaa ccgtatttgc
     2581 ccgcccaagc gatcgctttt ttcataaagg aatacccggt gccctctttt ccccagccat
     2641 tgggcggcct gtaggcccgc gattcccccg ccgacgacca tcactttttg ggaagtcgcc
     2701 gccggcttga tttctctcaa ttccgcttcc cgccccgccc aaggattgat ggcgcaacgg
     2761 acctgtttca tcgcccgcat gcgcttcccg tggcaatagt tgcaggcgat acacgacccg
     2821 atgtcttgaa tttgaccctg ctcaattttt tgcgggaaat aggggtcggc gatcatctgt
     2881 cggcccaaat ccaccaaatc gactttcttc tgggcgatga tgtctcgggc cagaaaggga
     2941 tcattgattc ttccggcaac gctgacggga atgttcagcc cctttttaat ttccgctgcg
     3001 atctccacgc ggcacccccg gggaatatac atgggcatga tcatttgccc gtaggattta
     3061 ttgtccccca acgaaagatc atgggccaag attcccactc ctcctccgat ggaaatcaca
     3121 tcggcccctg cggcttcaag aatgggggcg gcgacccggg catcctccac ggtaaacccc
     3181 ccagggagaa aatcctctcc attcatccgg aagaaaatgg ggaaatcgtc ccccaccgta
     3241 cgccgcacag acctgactac gtctgcggca aagtgggttc ttgctttgcg gtcgcctcca
     3301 taggcatctt tccggtggtt ggtgagagga gagagaaaag agctgatcag gtatccatgg
     3361 gctccgtgaa tcatgacccc gtcaaattcc gctttcttga cccgctccgc agcttgggag
     3421 aaccatacaa tcagttcctc catctggtcg gctgataaat catcgggccc gggggtattt
     3481 ccgcggatgc ctgtctggtt gatctgggca atggccgcgg cccccctttc cttgatgctg
     3541 gtggcgaggt tctcgagttc gttgataaac cggtcatgaa aaacgcccag ccggttgaaa
     3601 agcattcgat catcgatatc catggcctct accacgatgg cggcaatccc ccctttggct
     3661 ctttccacgt agtaatcgac catccggggg gttacgaatc cttggctgtt ggccaggcac
     3721 gtgaccatgg gagccatgac catcctattt ttcagggtta atttttttac cccccgaccg
     3781 atgagcaaag gctcaaaaag aggggaaaaa tcagttccca tgccgggcgc ctccttccga
     3841 ccgcatctcc cttttcatgg aatataccaa atgaccagcg ataccgaaag gggattcgag
     3901 ctttctccca acccaaaaat ctggagcccc gggaaggcgc cgctggggca ggttcatcga
     3961 ccaggccttc ggcccccggg ctacgagaaa agacatcgcc caagggaaga gggcctagtc
     4021 attttttccc ttgaccctcc catccgattc ttcgtatact cgggtcactc caaggccaaa
     4081 cttcagaatt cggcgttatt catcgacggg tccagcttcg tttccgggcc gatcctcatc
     4141 tctacggctt tcggggtttt ctctatggct aagcgctttc aataaaagga gtcctgggca
     4201 tggctttcct cgaagtgaaa gacttggtca aagattttgg cgggctgagg gccaacgatc
     4261 atgtcgacct tcaggtaaac caaggagaaa tcgtagggtt gatcggaccg aacggggccg
     4321 ggaagaccac tcttttcaac tgcattgccg gctattatcc tcccacttcc ggcgcagtca
     4381 ccttccaagg ggaaaacatc accgggctgc cggcgtacga aatttgccgc cggggaattg
     4441 cccgaacatt tcaaattgtc cgggtcttca aggatatgcc cgttctggat aatgtcatgg
     4501 tgagcgcctt caaccgcacc aaatcagcgg ccgtagcccg gaggaaagct ctggaggtgt
     4561 tggatttctg cggaatgggc tccaaaaaga tgatgctcgc cgcctccctc accattgccg
     4621 ataagaaaag gctggaagtc gccaaagccc tagctacggc gccggccctg cttatgctgg
     4681 acgaagcgat ggccggcttg aatcagaccg aaaccgcgga ggccgtggaa ttggtgaaaa
     4741 agatggagaa agccggcctg accatcctcc tggtagaaca tgtcatggaa gtcgtcatgc
     4801 ctatttccaa gcgggtggtg gtgctggatt atggccgaaa aatcgctgag gatgcgccgg
     4861 agaaagtcat acacaaggaa gaggtcatca aagcgtacct aggagacaag taccatgcta
     4921 cgcgtcgagc gccctagagt atccgatgcc ggagtaccgg ctcttcacgg ggtatccttc
     4981 aatgttgagg gagtagattg tccccatagt ggaggccaac ggggcgggaa aatccaccat
     5041 cagaaggaga gccagaatcg tcagccgcac gatctttaat tccaaaggag ggaaatgcag
     5101 atgagaagaa aaacgatcgt tttggccggg attttcatca tggctttggg gattttgagt
     5161 ccatcggtac aggctcaacc caaagaggtt ctcatcggag gaatttggcc cttaaccggt
     5221 ccgaatgctc ccacgggggt agagtgcacg cagggagcca cgctggccat ggaaattatc
     5281 aatgggaaat acgatttgaa tctacccctc gccaagacgg agggtttgcc caacctggga
     5341 ggcgcgaagc ttaaattggt tttcggcgac caccagaatg ttccggacaa ggccatggcg
     5401 gaggccgagc gtctgatcac ccaggagaag gtgatggccc tcataggatg ttacatcagc
     5461 ggcgtaaccg ctccggcctc ccaggcggcg gaacggcgca aagtggtatt cctcgacgcc
     5521 gatgccatag cccccacgct cattaagaga gggttcaagt attttttccg ggtcacccag
     5581 gatgatgaaa tttggttccg ggatttcatg tggacccgtt tcatccccga ggtgctcaaa
     5641 aagaaaggcg gaagcatcaa gaacgttgcg gttctccacg agaataccct ttggggaacg
     5701 gatgcctcca agtacgggac cgaatatgcg aagaaaaacg ggttcaacat cgtaactcaa
     5761 atcgcctacc cggcgcgttc cacgagcctc acggccgagg ttcagaagct gaagatggtg
     5821 gaaaagcaga ccgatatgct cctcagcgcc tgctacaccg ccgacgggat cctgctcatg
     5881 aagagcttcc acgagatgga ttataaccct cccatgatct ggaacgacgg ggggtatcgg
     5941 gagcccgcct tcctggaagc cctggggaaa aaatcggaat tcatcctcca gcgcgatgaa
     6001 ttttcggtcg acatggggga aagaaaactc ctggtcaaac aggtgaatga tcttttccgg
     6061 aagcgcttcg gcgtcgatat gaacaccaac tcttcccgta atttcatggg ggtcatctgt
     6121 ttggccgatg ccatcaaccg ggcgggttcc acggactccg aagccatccg caaggctctc
     6181 ctggcgaccc aaatccctcc agaacaatgc atctgctctt gggacgggat ccaattcgac
     6241 cccaacaacg gtcagaatat caaagctgta tccctgatgc tccaggtgca cgagaacaaa
     6301 tggcgattgg tgtggccctt cgaggtggct gtgaaggacc tggcgtaccc gatccccaag
     6361 tggtctgcgc gcccctaagg attgcttgtt tccccgggcc gggggcgggg gaccgccgcc
     6421 cccggccggt cccaagagga tttccatgct aaccaccgat atcattcttc agaccttcat
     6481 cagtggcctg ctgatgggtt gtatctatgc cctggtggct gtcggcctct gcctgatttg
     6541 ggggttgatg gagattgtca attttgccca tgggcaattc atgatgctgg ccatgttcct
     6601 gtccttctgg atgtatacct tattcggagt cgacccgctc ctgtccattc ctctgggagg
     6661 tctctgcctt tttatcatcg gagtcgccac ctatcatctg attatcaaag gaatcctcta
     6721 taccaccttc ttcgcccaga tctttgccac ctttggcttg gggatttttc ttcatagcgc
     6781 cgcccagttc ctttggacgg cgaattaccg ctccgtccag aatcccctcc tggcggggcg
     6841 ggtggccttg ggaaacattt ttatcggatt cccccaagta gctgcggcga tcatgtgcat
     6901 ccttggtttt ctctttctct attggctgat caacaaaacc gagattgggc tggccttgca
     6961 ggcggcgagc gaagaccggg aggctgcctc cctcatgggg atcaacagca accgaatttt
     7021 cgccctgggg tgggggattg gatcggcctg cgtggggatt gccggggttc tcctcaccaa
     7081 cttcttctat atcttccccg aggttggttt tacctttgcc ctgatcgcct atgtggccgt
     7141 ggcgctggga ggttttggca gtatcgtggg ttcttttctg gccggggtca tcatcggctt
     7201 ggtggaggcc ttcgccgggt tgttcattga cccggccttc aagtattttt cgatttacgt
     7261 actctacatg gtggtggtct ttttccggcc gcaggggctt tggggccggt actgatcccc
     7321 cccaaaggag aaaccttccc gagattcgag gaaagggtct atgatgaaga tgatagggtt
     7381 cgttggggtg ggtatttttc ttctgctttt cccgttcatt ttcaccctgc cattccccca
     7441 gcacattatg atcatgatct ttgtatatgc caccttggcg caggcctgga acattgtcgg
     7501 cggatacacg gggcagtgtt ccctcggtca tgcggttttt tttggctgcg gggcctacac
     7561 ctccagtctt ctcctgatcc aattgggact taacccctgg ctgggaatgt tggccggagc
     7621 gattttggcc gtgggcatct cgttccttgt ggccattcct tgttttcgcc tcgccgggca
     7681 ttactttgcc attgctacca tcgctatcgg ggaaatatgt cagatcggca tggtcaattg
     7741 gaaaggcggc gggggggcga acgggctcta tttacccatt ctcgaggagg gttttcttaa
     7801 tctggaattc cataacacca aggttcccta ctactacatc gctctgggaa ttgcggcgat
     7861 cacctttgct gtggttttct tactggaaag gtcctttctg ggttattatt ttcgggccat
     7921 caaaggagac ctggatgcct cccgcgccgc aggcattcat gtcgcccggt acaagacggt
     7981 ggccctggcc atcagcgcct tctttacttc gatcaccggg agtttctatg cccagtacgt
     8041 cctgttcatc gacccggaaa gcgtactccc cctcatgctt tccgtgttga tcagcttggt
     8101 ggccattctc ggtggagtgg caacggtgtg ggggcccttc ctgggagccg ctgtgctgat
     8161 ccccctttcc gaattcaccc ggacctatct gggaggcagg ggaacggcgg ttgacttgat
     8221 gatttatggt ttactcatcg tcctcatagc taggtttcaa cctaccgggt tgatggggct
     8281 attgaagaag cggtaggaaa ccgacgggcg gttcatcccg tacgcttcga actaatctgg
     8341 aaagaattcc caaactctta ccggtaaaga aaggaaaaac cgatggaccg agaaaaattg
     8401 cgggatttgc cccccaggga agtccgtcaa ctcatccgcg agggaaaatg gcggaaggga
     8461 accgcctttg tttccgcggg at
//
LOCUS       DASPRR010000040         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2269500, whole genome shotgun sequence.
ACCESSION   DASPRR010000040 DASPRR010000000
VERSION     DASPRR010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269500"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="VEL68_01535"
     CDS             <1..130
                     /locus_tag="VEL68_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HXZ34680.1"
                     /translation="LKAGDISYLPSGHDAWVVGNEPVVVVDFQGMVDYAREAAKKR"
     gene            complement(100..1143)
                     /locus_tag="VEL68_01540"
     CDS             complement(100..1143)
                     /locus_tag="VEL68_01540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ34681.1"
                     /translation="MNGLRALASTDPASVFSLDLPLDLDLRNGLKGLQESLKIPWMIW
                     FVDDPEGFDFPDCLDPDWTIVFCWDGEITRQISADGSWKGIPPIHLPLATDPEVFYPE
                     GNGSPLLFPGGVFIGSTAHPNRFFDEAILNSPGFEEIVSTLWEAWKANLGQVPQEVVW
                     GFLEGKTGIGLSTLKKDPLARLWAHSTAYALGRRKRRGIVSTVIGQGGGVFGDREWEQ
                     AVGNTYRGKVIYGEELRRIYLASAFVLETRQSQCRTGLTQRVFDASACGIPVLAEHSP
                     ELDEYFNPREEIFSFRTLEEALERKKNILFLQNHSPLAVRARNRVLAQHTYRHRAARI
                     LEEIQRFFAASRA"
     gene            complement(1336..2325)
                     /locus_tag="VEL68_01545"
     CDS             complement(1336..2325)
                     /locus_tag="VEL68_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ34682.1"
                     /translation="MPGKARLSAPVVLADRFYMSEKALADISSLGGYLWAEAKDGNDL
                     ADKIGGSPSVKVIVSEYVPVNLQLLSCAPGLKGVIAYGAGYDHIDANALSRKGIKVCN
                     CRGENAQAVAELAFGLLLSLLRRIHRADPWVRTGDWVKAGRALPEWASGQEIWKKTLG
                     IIGLGQIGARVVRIAKGFEMKIIGYDPFIKPELYDRMGVEPASLAELLSRADVVTLHV
                     PLSKATEKLLDSHALALVKPGMIMVNTSRGKLIEEGALIEALQTGRIKGAALDVFATE
                     PLPSGHPLTRLENVVLSPHMGALSQEAGDRLSDSVARQVRDILEGRPPECLIA"
     gene            complement(2315..>3082)
                     /locus_tag="VEL68_01550"
     CDS             complement(2315..>3082)
                     /locus_tag="VEL68_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ34683.1"
                     /translation="EWVKVGAIKIFLDGGMSSRTAAVSKAYEFPPGAGRGILNYDQKG
                     INREIEKFDRAGYQVSVHAQGDRALEMLLKAFERAIDRGNPLRHQIVHAGNLTAPQID
                     RVAKLGLYVSTQANFFSLLGDGFMEAFGPIRSRELYRFRTLLQKGVRLGLSSDCPVAD
                     PNPLIGLRDAICRKTGKGQDFAPAECLTAEQAVSLYTREAAHFSFEEGQRGTLKEGKA
                     ADLVILDRDPLQTEPETIPEIKVKMTMVGGRIVYDAR"
BASE COUNT          688 a          900 c          822 g          672 t
ORIGIN      
        1 cctgaaagcg ggggatattt cttacctgcc ctccgggcat gacgcctggg tggtgggaaa
       61 cgagcctgtg gtggttgtgg attttcaggg catggtggat tatgcccggg aagccgcgaa
      121 aaagcgctga atctcctcaa ggatccgggc agccctatgg cggtatgtgt gttgggcaag
      181 gactcggttc cttgcccgca cagccagagg ggagtggttt tgaaggaaaa ggatgttttt
      241 tttcctctcc agcgcttctt cgagtgttcg gaaagaaaag atctcctccc gggggttgaa
      301 gtattcgtcc agctccggtg aatgctctgc cagcactgga atcccgcagg cgctggcgtc
      361 aaagacccgc tgggtgagac ctgttctgca ttgggattgc cgggtctcaa gaacaaaggc
      421 gctggccaga taaatccgcc gcaactcctc cccgtagatg actttccctc gataagtatt
      481 tccaacggcc tgttcccatt ctcggtcgcc gaatacccct cccccttggc cgataaccgt
      541 cgaaacgata cctcttctct ttcttcggcc gagcgcgtaa gcggtggaat gtgcccaaag
      601 gcgggcaagg gggtcctttt tgagagtact cagcccaatt ccagtcttcc cttccaaaaa
      661 tccccagacg acctcctggg gaacctgccc caggtttgct ttccaagcct cccacagggt
      721 tgaaacgatt tcttcaaagc caggagagtt caggattgct tcatcgaaaa accggttggg
      781 atgggcagtg gaccctataa aaaccccccc ggggaagaga agaggagagc cgttcccttc
      841 aggatagaag acctccgggt ctgtggccag gggaaggtgg atcgggggaa ttcccttcca
      901 agaaccatcg gcagaaattt gccgggttat ctccccatcc cagcaaaaga cgattgtcca
      961 gtcggggtcg aggcaatccg gaaaatcgaa tccctccgga tcatcgacaa accagatcat
     1021 ccaaggaatt ttgagggatt cctgaagtcc cttgagaccg tttctcaagt ctagatcaag
     1081 gggcaggtcc agagaaaaaa ccgatgcagg gtcagtggag gccaaagccc tcaacccgtt
     1141 catatctgct tttccccggg aataagggaa gtccaaaact tcatgcccaa gggaacggaa
     1201 ggctttgatc atggaggaat gaatgaaggg aataaccgta ccctggggtt ttggggatag
     1261 aattcgcatc gggaaaggct cttgaatttg tctcttttgg aagggggttg gtgcaaggaa
     1321 aggagtagcc cgcgcttacg caatcaggca ctccggcggg cgcccttcca gaatatcccg
     1381 gacctgccgg gctacggaat cggaaagtcg gtctccagcc tcctggctca gggctcccat
     1441 gtggggagaa aggacaacat tctccaatct ggtcaaggga tggccggaag gcaggggttc
     1501 agtggcaaac acgtccaaag ccgccccctt gattcgtcct gtttgaaggg cttcgatcag
     1561 cgccccttcc tcgatcaact ttccccggga ggtgttaacc ataatcattc ccggcttcac
     1621 caatgcaaga gcatgagagt cgagtaattt ttcggtggct ttagagaggg ggacatgaag
     1681 ggtaaccacg tcagccctgg acaggagctc ggccaatgat gccggttcca ctcccattcg
     1741 atcataaagt tcaggcttaa taaaaggatc ataaccaatt attttcattt caaagccctt
     1801 tgcaattctt accactcttg ctccaatctg tcctaacccg atgatcccca gggtcttctt
     1861 ccatatttcc tgcccggaag cccactccgg caaagccctg cccgccttga cccaatcccc
     1921 agtcctaacc caaggatcgg cccggtgaat ccgtcgcagc agagagagga gaaggccgaa
     1981 agccagctcc gcaactgcct gggcgttttc accccggcag ttgcagactt ttattccctt
     2041 ccggcttaag gcgttggcgt caatatgatc gtaccccgcc ccataggcaa tgactccttt
     2101 cagccccggg gcgcaactta gcaactgaag attcaccggg acatactccg agactatgac
     2161 ctttaccgag ggcgacccac cgattttatc cgccagatca ttcccgtcct tcgcctcggc
     2221 ccagaggtac cctcccaaag aggagatatc ggccaaagct ttttccgaca tgtaaaaacg
     2281 gtcggccaga acgaccggag cagagagcct cgccttaccg ggcatcgtaa acgattctcc
     2341 ctcccaccat ggtcattttc actttgatct cgggaatcgt ttccggttct gtctgcagag
     2401 gatccctgtc caggatgacc agatcagcgg ctttcccctc tttcagcgtc cctctctgcc
     2461 cctcctcgaa ggagaaatgg gctgcctctc gggtgtagag agacactgcc tgttccgcag
     2521 tcaggcactc cgccggtgcg aaatcctgcc ccttgcccgt cttccgacaa atggcatccc
     2581 ggagaccgat cagcgggttg ggatcggcaa ccgggcagtc cgaagaaagg cccagccgca
     2641 ctcccttttg gagaagagtc ctgaagcgat acagttcccg ggatcgaatc ggcccgaaag
     2701 cttccatgaa accatccccc aggagggaga aaaagttggc ctgggtggaa acgtacagac
     2761 ccaatttggc cacccggtcg atctgcgggg cggtcagatt ccccgcatgg acgatctggt
     2821 gacgaagggg gttcccccta tcgatcgccc tttcaaaggc tttcagaagc atctccaggg
     2881 cccggtcgcc ctgggcatgg acagatacct ggtaccccgc ccggtcgaat ttttcgatct
     2941 cccggttaat tcccttctgg tcatagttca ggattcctct tccggccccg ggagggaatt
     3001 cataggcctt ggatacggcc gccgtgcggc tgctcatccc cccgtccaga aagatcttga
     3061 tcgcccccac cttgacccac tc
//
LOCUS       DASPRR010000041         4961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_493676, whole genome shotgun sequence.
ACCESSION   DASPRR010000041 DASPRR010000000
VERSION     DASPRR010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493676"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VEL68_01555"
     CDS             <1..758
                     /locus_tag="VEL68_01555"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HXZ34684.1"
                     /translation="AALGRGHEPDCLERWIWPPSAASLPRAALVYQVQSQGDLRRTFV
                     YGQPADSILPTLLNPLEVLDGAVVSGNFVLPSNKTCTYIHQNHPVVLEMLRRHGSDLA
                     FAGVILSNEASLLPDKERSAQFAEKLARLLSVQGVVINQEGGANTLADVMLLCRLLTQ
                     SGMKTALLVNEFAGSDGRTPSLAETTPEATAIVSTGNNDHRITLPSRKEFIGFSPLQG
                     MEGDVAGEVTVPLARIYASTNQLGFNSLSCRTR"
     gene            938..2233
                     /locus_tag="VEL68_01560"
     CDS             938..2233
                     /locus_tag="VEL68_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1973..1975,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HXZ34685.1"
                     /translation="MRIVHYLNQFFGQIGGEDKADAPCQIKPGPIGPGILFQQLFGDR
                     GEIAATIICGDNFAAQSLEETGQTVAAMAVEHAPDLFLAGPAFGAGRYGLACGAACQA
                     VGRRLGISTVTGMSEDNPAVQVYCSHSYIVPTGRSSAGMREAAAKMVELSICLAEGRV
                     LPEGAHFPRGIRELVVLESSGAKRAVDMLVAGLAGRPVITELPLPRFEKVTPAPPLED
                     LTQTTIILVTEGGIIPAGNPDGIEMSMATKFGKYALSGLDRMTSERFTVAHGGYDNQY
                     AKADPNVLIPLDVLRDLVAEKKIAALGEAFYATAGNATSVENSCRFGKEIAQDIRKQH
                     KEKVGVILTATUGTGTRCGATMAKELEAAGIPTTLISSLVSIAQNVGANRIVPGLGIP
                     HPLGDPRLKPEEQKKVRRALVERALEALASPISGQKIFP"
     gene            complement(2858..3223)
                     /locus_tag="VEL68_01565"
     CDS             complement(2858..3223)
                     /locus_tag="VEL68_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2984..2986),aa:Sec)
                     /transl_table=11
                     /product="rhodanese-related (seleno)protein"
                     /protein_id="HXZ34686.1"
                     /translation="MTSKVHAMGKGVEVPRVTKEQLLSMMGKPEVVILDVRESESWKK
                     SKQKIQGAVREDPLKDVQGWFDKYPKDKTLVFYCSUPNEETSAWVARQFVSRGYDKVF
                     VLKGGWDEWEKAKFPVEPK"
     gene            3586..4050
                     /locus_tag="VEL68_01570"
     CDS             3586..4050
                     /locus_tag="VEL68_01570"
                     /inference="COORDINATES: protein motif:HMM:NF021896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide isomerase DsbC N-terminal
                     domain-containing protein"
                     /protein_id="HXZ34687.1"
                     /translation="MVRYFLLLFSVVFLACALLQLNPNPLRAMDQTGCEKDCRKCHTI
                     SNEEIKEILKKLKAPDAEIVKVQDSPLKGLWEISIINRGRPGLFYVDFSKAFVVSGSI
                     VEVKSGKNKTVEQLTKLQESRKVDFSKIPLDQALVMGDPVAPKKVAVFTDPD"
     gene            4063..4413
                     /locus_tag="VEL68_01575"
                     /pseudo
     CDS             4063..4413
                     /locus_tag="VEL68_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
     gene            4534..>4961
                     /locus_tag="VEL68_01580"
     CDS             4534..>4961
                     /locus_tag="VEL68_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34688.1"
                     /translation="MKENRPSHDTLPVGLIGLGSMGSFVAREIMRGEIPGILLVAAAD
                     IRPPSPDLLQELRKHSVSLVQSFEGLGNFPIRLVIECANQKVVTECADFFLAKGIDLL
                     LMSVGALVQGSLFSQLTARAEEKGCRIYLPSGAIGAIDAL"
BASE COUNT         1169 a         1325 c         1348 g         1119 t
ORIGIN      
        1 tggctgccct gggccgaggt catgagccgg attgcttgga aaggtggata tggcctccat
       61 cggcggcctc tcttccccgt gcggctttgg tctatcaggt tcaatctcag ggagacctgc
      121 ggaggacctt tgtttacggc cagcccgcgg actccatcct tccgaccctg ctcaatcccc
      181 tagaagtatt ggatggggcc gtggtcagcg ggaacttcgt tctcccgagc aataagacct
      241 gcacttacat ccaccagaac catcccgtgg tcctggagat gcttcgccgc catggatcgg
      301 acctagcctt tgccggggtg attttaagca atgaggccag tctattaccc gacaaagaaa
      361 ggtcggccca gttcgcagaa aaactggccc ggctcctcag tgtccaggga gtggtgatca
      421 atcaagaggg aggggcgaac actctcgccg atgtcatgtt gttatgtcgc cttctcactc
      481 agtctggaat gaagaccgcc ctcctggtca acgagtttgc cgggagcgat gggagaaccc
      541 cctccctggc agaaacgacg cctgaggcga cggccatcgt gagtaccggc aacaatgatc
      601 accgaattac gctaccgtcc aggaaagaat tcatcggttt ctcccccctt caagggatgg
      661 aaggggatgt ggccggggag gtcacggtac cgttggccag gatttacgcc tccaccaatc
      721 agctggggtt caacagcctt tcttgcagga cccggtagct ttcccaattg ggaatttgga
      781 atgccgaagg tagaaactcg ccgtgggcta atgaaaactg gaattcgcca aggcgacggt
      841 tgcacgcttc gagcgatggc ccctccttta tcctctgcgg ggccgggaaa ttcggatttt
      901 cattgggaat atcggaagta aaagcatgcc ccacccgatg cgcatcgttc attacctcaa
      961 ccagttcttc ggacagatcg gtggcgagga caaagccgat gctccttgcc agataaaacc
     1021 aggtcccatt ggccccggaa ttcttttcca acagcttttc ggcgaccgcg gggaaattgc
     1081 ggctacgatc atttgcgggg acaactttgc cgcgcagagc cttgaagaga caggacaaac
     1141 tgtggccgca atggcggtgg agcatgcgcc ggacctgttt ctcgccggtc cggcattcgg
     1201 tgcgggtcgt tacgggttgg cttgtggggc ggcctgccag gcggtgggaa gacgcctggg
     1261 catttcgacg gtcactggga tgagtgagga caacccggct gtccaggtct attgttccca
     1321 ttcctatatc gtccctacgg gtaggtcatc ggcagggatg cgggaagcgg ccgcaaagat
     1381 ggtggagctg tccatctgtc tggctgaagg acgggttctc ccagaaggag cccatttccc
     1441 gcggggaatc cgggaactgg tggtgctgga gtctagcggc gccaaacggg cggtggacat
     1501 gctcgtggcc ggcctggccg ggcgacctgt tattaccgag cttccgctcc cccggtttga
     1561 aaaggtcacg cccgcaccac ccctggaaga cctcacccag acgaccatta tcctggtcac
     1621 ggagggaggg atcatcccgg cgggaaatcc cgacggaatc gaaatgtcca tggccaccaa
     1681 attcgggaag tatgccctgt ccgggttgga ccggatgact tcggaacgat tcaccgtggc
     1741 tcacgggggg tacgacaacc agtatgccaa ggctgacccg aatgttttaa ttcccctgga
     1801 tgtcttaaga gacctggttg ccgagaaaaa gatcgctgcc ttgggggagg ctttctatgc
     1861 caccgcgggc aatgccacct cggtggagaa ttcctgtcgc tttggaaaag aaatcgccca
     1921 agatattcgc aagcaacaca aggagaaggt cggggttatc ttaacggcca cctgaggaac
     1981 cgggactcgt tgcggggcaa cgatggccaa agagttagag gcggccggaa ttcccaccac
     2041 cctcatttcc agcctggtct ccatcgctca aaatgtgggg gccaatcgca ttgtaccggg
     2101 actcggtatt ccccatccac tgggagaccc ccgccttaag cccgaggagc aaaagaaggt
     2161 tcgaagggct ctagtagaga gggctttgga ggctttggct tcgcctatat cgggtcaaaa
     2221 gatttttccg taagagattc aattttttgc ccctctccga cctttacact ctttacaaag
     2281 tgttggtaaa tcttacgcca attctttcca aaaaaattcc ttctcaacca ataatgtttt
     2341 aatttcaaga ccttagaaat ttggcacata tattgctatt aattaatgat tttcagtctg
     2401 aaatcatatt attgggtgat aggaagaaag ggggaaggct gagaggtggc aagtaaaatc
     2461 attttattct ccggcgcaat tattctttta ttgacggccg gagccattgc tagtggggta
     2521 cctaatatcg tcacattaca ggctccggaa cccaccgtct tggtcctgat gggcctcgcg
     2581 gttattgccc tgcggaggtt gcgaaagaag ctggattaga aggaatcaaa ggaaatcaat
     2641 agactccaaa aggcagggcc aaatcggctc tgcctttttt tccgggcaga aggggaattc
     2701 cggactttca ttttcttctg gcccgtgtgt cgatttctac cattaaaatc aaaattaggg
     2761 acatgaccgg cagcccatag aggaagaagg gattcccgaa gaactgaccg tgctcttcct
     2821 ccggatgcaa gaaattcacg ccgcatgaaa taagggatta tttgggctcg accgggaact
     2881 tggctttttc ccactcatcc cagccgccct tcaatacgaa gaccttgtcg taaccacggg
     2941 atacaaactg ccgcgccacc caggcactgg tctcttcgtt gggtcaggag caatagaaaa
     3001 cgagggtttt gtctttggga tatttgtcga accaaccctg aacatccttt agggggtcct
     3061 cgcgcactgc cccctgaatt ttctgcttgc tctttttcca tgactcagac tcgcgcacgt
     3121 cgagaatcac cacctccggt ttccccatca tggagagtag ctgttccttg gtaacccggg
     3181 gaacctccac tccctttccc atggcgtgga ccttagaggt catcccgctg agaaccaaaa
     3241 agatcgccaa caaaatagaa gagaaataac gtcttggctt catgatttcg ctccttccat
     3301 cgattcatga aaaaacggtc ttatactttt agcctaagag atttttgcga ccaacgcaag
     3361 aggcatccgc agactcggga cggacctcca agggcccggg ttgagttcaa gaggagggag
     3421 ttcggctgga tgaggtcccc aaaggaaaaa gcgaatggcc cggggataga gaattttgcc
     3481 actcccagcc gataaatttt ttaaaagacc cttttgggaa tgaaaattgc aaaaattatg
     3541 gagtataatt gatattaatc tcgaaaggaa cggagacaga cccttatggt tcgatatttt
     3601 cttctcctgt tttcggttgt atttctggcc tgcgccctcc tccagctgaa tcccaacccc
     3661 ctccgggcca tggaccagac tggctgtgag aaagactgcc ggaagtgcca caccatatcc
     3721 aatgaagaaa ttaaggaaat cctcaaaaaa ttgaaggctc ctgatgcgga gatcgtaaag
     3781 gttcaggata gcccgctgaa aggcctttgg gagatttcga tcatcaacag gggaaggccg
     3841 ggcttgttct acgtggattt ctccaaggcc ttcgtagttt cgggctcgat cgtcgaggtg
     3901 aaaagcggca agaacaagac cgtggagcag ttgaccaagc tgcaggagag tcggaaagtc
     3961 gacttctcca aaatccctct agatcaggct ttggtcatgg gggacccggt cgctcccaag
     4021 aaagtcgcgg tttttactga cccggactga ccctactgcc tcacactcca tcaggagatg
     4081 gagaaggtta tcaaagagaa taaagacatc gttttttaca tcctcctcta ccctctgagc
     4141 ttccataagg acgcctactg gaaatcgaaa agcattatgt gcaaccggtc cctgaagatg
     4201 ctggaagacg ccttcgccaa gagggagatc cccaagctgg aatgcgattc caaagagatt
     4261 gattccaaca ttaaagtcgc cgaagccctg ggaatcaccg gaactccggc tctggtcctg
     4321 cccgacggcc gggtacacac cgggacgatg cctgccaggc agctgatcga cttcatcaac
     4381 ggaattcccg gacccgagcc caaagcgaaa taatttgacc cggagacacg gagaaacgaa
     4441 gacgcggaga aatacgtctt tttgtccatg tcaaccaatc cccgcgtccc cgtgccgatt
     4501 taaaatgacg acccagacta ccgaggaggc gccatgaaag agaatcgtcc ctcgcatgac
     4561 acgctgcccg tgggcctcat cggcctcggt tctatgggga gctttgtggc ccgggaaatc
     4621 atgagaggcg aaattccggg aatcctgctg gtcgcagcgg cggatatccg ccctccatcc
     4681 cccgaccttc tacaagagct gcgaaagcat tccgtctccc tggtccagtc ctttgaggga
     4741 ttggggaact tccccatccg gctggtcatc gaatgcgcga atcaaaaagt cgttaccgaa
     4801 tgcgctgatt ttttcctggc caaggggata gaccttttgc tcatgagcgt gggggctctg
     4861 gtgcagggtt ctctcttttc ccagctgact gcccgggccg aggagaaggg atgccgcatc
     4921 tatctcccct ccggggccat aggggcgatc gatgctttgc a
//
LOCUS       DASPRR010000042         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_659523, whole genome shotgun sequence.
ACCESSION   DASPRR010000042 DASPRR010000000
VERSION     DASPRR010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659523"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VEL68_01585"
     CDS             <1..1254
                     /locus_tag="VEL68_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ34689.1"
                     /translation="REMAQVKPKALIHRGYHGAFGAQYLNSFQTARALAIANALLGNI
                     DREGGIYFPQTAQLGELQPKHPVPEGPKVPKSDGTGIPGRYPLGSYGDGIAHAIPELA
                     LRGELKAGFVYHYNPLRTNPNPKRVIAGYQKLDLLVAIDTVLSETASIAHYVLPESFY
                     LERDEAVDTKHSGKIAQVSIQQQVVKPLFDTKPGTQIIFELAKRLGLGKYFEFDLEEA
                     NRLRLKPLGVTLEDLKIRGVLPVGKEWKEGFEKLDTPSGKVEIYSSTLEKLGYPPIPR
                     WEEPLVTPNPKDPHAFRLLHGKQAVHSHAMTANQPYLLEISRRYDLIRLWMNRQRGEK
                     VGLKDGDWAKVKSLVGEGKIRVKLTEGLHPSAVWLPSGYGNFSQHLKNSYDVGLSYND
                     FLPTLFDPAVGHAMSAEVIVQVTKV"
     gene            1271..1810
                     /locus_tag="VEL68_01590"
     CDS             1271..1810
                     /locus_tag="VEL68_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ34690.1"
                     /translation="MARYGMVIDETKCVGCHACRVACQNQNSLTERESYNHLEEREYG
                     AFPNYNREFIPVQCQHCDNPPCVRVCPTGASYKRKDGIVLVDKDQCIGCKYCIAACPY
                     NVRIIKKEGYIEKCRFCLEFVEKGEKPACVTTCMTGVRVFGDLDDPNSEVSRLVIKQK
                     LKQFKADLNTRPRIYYKKA"
     gene            1816..2457
                     /locus_tag="VEL68_01595"
     CDS             1816..2457
                     /locus_tag="VEL68_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HXZ34691.1"
                     /translation="MTSREKELLCELMAAVFSPPEAEGIEQIRREGFILEESVKSWGG
                     DAALLFGLKTEGEAEALSRDLRGEYDRLFGGKTGDSAPLVESCYKPWTQDGECRLSFA
                     HENGLLMGDPALHMAHIFRHSGLEVPKPFQACPDHLVLELEFLSVLYGNATDREVNQF
                     IQDHLDWVPQMKRDLKQLCPHPFYLSAAELVDDFLRHEKNRLETRDDGKKSLY"
     gene            2432..2695
                     /locus_tag="VEL68_01600"
     CDS             2432..2695
                     /locus_tag="VEL68_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34692.1"
                     /translation="MMERRAFIEKLIAAISLGGVFLLAACQNKEDRIPRSGSEDPLWK
                     LAQGQAKVEEPLELAYSKGTPAFYRDASLGKIDPSFAPKTGGG"
     gene            2825..3844
                     /gene="nrfD"
                     /locus_tag="VEL68_01605"
     CDS             2825..3844
                     /gene="nrfD"
                     /locus_tag="VEL68_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXZ34693.1"
                     /translation="MEGEIYWNWTVAVYLFVAGVSAGAFGISAFAYFFGEEKYEKITR
                     LGAYIAPFPLIFGLLCLIYDLERPHLFWKLLLTVQVNSVMSLGSWLLVLFSAMTFLHF
                     YLWLPERYDLVHLLNRLPKRWDGIKAVHWLKTNSFLVEFRRKNLTRNRGLVAGWGIPV
                     SMGVGIYTGVLLGALNARPFWNNPMLPFLFLLSAMKTGTAAICFAGCFLNGSGRMTQE
                     ELETNKFMIHTIDFTLMVFSVIAVFLFVMGLYTSPRSSVEAAHLIMGGDYTFPFWVIA
                     VGLGILVPMALETYELIPHFIKRVSLREHKPWLSGVTASLVLLGGFILRLVIVYAGQL
                     ARVVA"
BASE COUNT          874 a         1151 c         1124 g          858 t
ORIGIN      
        1 cgggaaatgg cccaggtcaa gcccaaggcg ctcattcacc gcggatacca tggggcattc
       61 ggagcccagt acctgaacag cttccagact gcgagagccc tggccatcgc caacgctctc
      121 ctggggaaca tcgatcggga aggcggcatc tattttcccc agaccgcaca gctaggggaa
      181 ttgcagccca agcatccggt tcctgagggg ccgaaggttc ccaagtccga cggcaccggg
      241 attccgggaa gataccctct ggggtcctat ggcgatggga tcgcccatgc catccccgaa
      301 ctggccttgc ggggagaatt gaaggccggc ttcgtttacc actataatcc cctgcgcacc
      361 aaccccaacc ccaagcgggt catcgccggg taccagaagc tagacctcct ggtggccatc
      421 gacacggtgc tttcggaaac ggcctccatc gcccactatg tcctgcccga atccttctac
      481 ctggaacggg acgaggccgt cgacaccaag cacagcggga agatcgccca ggtcagcatc
      541 cagcagcagg tggtaaagcc cctcttcgac accaaacccg gaacccagat tatcttcgaa
      601 ctggccaagc gtctgggctt ggggaagtat ttcgaattcg acctggagga ggccaaccgc
      661 ctgcgcctca agcccctggg cgtgaccctg gaggatttga agataagagg cgttctgccg
      721 gtaggcaagg agtggaaaga gggattcgag aagttggaca ccccctcggg gaaggtggag
      781 atctactctt ccaccctgga gaagctgggc taccccccga ttccgcgttg ggaggagcct
      841 ttggtgactc ccaaccccaa ggacccgcat gccttccgcc tcttgcatgg caaacaggcc
      901 gtccacagcc acgccatgac cgccaatcag ccctatctcc tggagatcag ccgccgctac
      961 gacctgatcc gcctgtggat gaaccgccag cggggggaga aggtggggtt gaaggacggc
     1021 gactgggcga aagtcaaatc gctggtggga gaagggaaga tccgggtcaa actcaccgag
     1081 ggactgcacc cctcggcggt gtggctgccc agcgggtacg gaaatttctc ccagcacctg
     1141 aagaactctt atgatgtggg gctctcctac aacgactttc tgcccacttt gttcgaccct
     1201 gccgtgggtc atgccatgtc ggcggaagtc atcgtgcaag ttaccaaggt ctgaggcagg
     1261 ggaaaaaatt atggctcgct acgggatggt cattgatgag acgaaatgcg tgggatgcca
     1321 tgcctgccga gtggcctgtc agaaccagaa cagtcttacc gaaagagaat cctacaatca
     1381 cctggaggag agggaatacg gggcttttcc caattacaac cgggaattca tcccggtcca
     1441 atgccagcac tgcgacaacc ctccctgcgt aagggtctgt cccaccggcg cctcctacaa
     1501 acgaaaggac ggcatcgtcc tggtggacaa ggaccagtgc atcggctgca agtactgcat
     1561 cgccgcttgc ccctacaacg tgaggatcat caagaaagag gggtatatcg agaaatgccg
     1621 tttctgcctc gaatttgtcg aaaaaggaga aaagccggcc tgcgtcacta cttgcatgac
     1681 cggggtccgg gtctttggag accttgatga cccgaacagc gaagtctcca ggttggttat
     1741 caagcagaag ctaaagcagt tcaaggccga tttgaacacc cgtcccagaa tctattataa
     1801 gaaggcctga tccggatgac ctcccgagag aaagaacttc tctgtgagct gatggctgcc
     1861 gttttctccc cgccggaggc tgaagggatc gaacagattc gccgggaggg ttttattctg
     1921 gaggaatccg tcaagtcctg gggaggagat gccgctcttc tcttcggact caaaacggag
     1981 ggcgaggcgg aggctttatc ccgggatttg cgcggcgagt acgaccggct ctttggcgga
     2041 aagacgggag attccgctcc cctggtggaa tcctgctaca aaccctggac ccaggatggg
     2101 gaatgccgtc tctcctttgc ccatgaaaat ggacttctca tgggggaccc cgccctgcac
     2161 atggcccata ttttcaggca ttcagggctg gaagtgccca agccgttcca ggcctgtcca
     2221 gatcatctgg tgctggagct ggaatttttg tccgtccttt acgggaatgc caccgaccgg
     2281 gaggtcaacc aattcatcca ggaccacctg gattgggttc cgcaaatgaa aagggatttg
     2341 aaacagcttt gccctcaccc tttttacctg agtgccgccg aattagtgga tgattttctg
     2401 cgccacgaga aaaatcgact ggagacccga gatgatggaa agaagagcct ttattgaaaa
     2461 gctgattgct gcgatctcct tgggaggggt tttcctcctg gccgcgtgcc agaataagga
     2521 agaccggatc ccccgctccg ggagcgagga ccctctctgg aagctcgccc aggggcaggc
     2581 caaggtcgaa gagcccctgg agctggccta ctccaaaggc accccggcct tctaccgcga
     2641 cgcctctctc gggaaaatcg acccgtcctt tgcccccaag acgggaggcg ggtgaagctg
     2701 atccctgtcc gccgtgggcg gatgttaaag acagtgaaag tgcaagtgaa agggtaagga
     2761 aggatcccta caaccaaaat atgcaatctt ttcactttca ctttcactga ccaaggagtt
     2821 aaggatggaa ggggaaatct actggaactg gaccgtggcc gtatatctgt tcgtggccgg
     2881 agtgagcgcg ggcgccttcg gcatctcggc cttcgcttat ttcttcgggg aggaaaagta
     2941 tgaaaagatc acccgcctcg gggcctacat cgcccctttc ccgctgatct tcgggcttct
     3001 ctgtctgatc tacgacctgg agaggcccca cctcttctgg aagctgctgt tgaccgtgca
     3061 agtcaactcg gtcatgtccc tggggtcctg gcttcttgtt cttttttcag caatgacctt
     3121 tctccacttt tatctctggc ttccggaacg gtacgacctc gttcaccttc tgaaccgact
     3181 gcccaaaagg tgggacggaa tcaaagcggt gcattggctg aaaaccaatt ccttcctggt
     3241 tgaattccgc cgcaagaatc tgacccggaa ccgcgggctg gtggccggct gggggatccc
     3301 cgtctccatg ggagtgggaa tctatacggg cgtgctcctc ggggcgttga acgcccggcc
     3361 cttctggaac aaccccatgc ttcccttcct cttcctcctt tcggcgatga agaccggcac
     3421 ggcggcgatc tgcttcgcgg ggtgcttcct gaacggctcg gggaggatga cgcaggagga
     3481 gctggaaacc aataaattca tgattcacac catcgatttc accctcatgg tcttttccgt
     3541 catcgccgta ttcctcttcg tgatgggact gtacacctcg ccccgaagtt cggtggaagc
     3601 cgcccacctg atcatgggtg gagactatac tttcccgttc tgggtaattg ctgtaggctt
     3661 gggaattctg gtccccatgg ccctggaaac ctacgaactg atcccccatt tcatcaaacg
     3721 ggtcagcctc cgggagcata aaccatggct ttccggagtc acagcctcat tggtccttct
     3781 gggaggattc atccttcggc tggtgattgt ttacgccggg cagctggcga gagtggtcgc
     3841 ctaaaacagt ctcaggtttc aggtctcagg tctcaagttt aaggttgccg atcgcaggct
     3901 taataaacgg accttcccct ccctcaaacc ctaaattact ctttgagttg gattcatttt
     3961 cttccgataa aatatttacc ggcacctgta attccgtgct tgttaac
//
LOCUS       DASPRR010000043         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_976615, whole genome shotgun sequence.
ACCESSION   DASPRR010000043 DASPRR010000000
VERSION     DASPRR010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976615"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..857)
                     /locus_tag="VEL68_01610"
     CDS             complement(3..857)
                     /locus_tag="VEL68_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXZ34694.1"
                     /translation="MKPFAYFEPATLEEALDLLHEYGERAKVLAGGTDLVVQMKQKKV
                     SPEAVISLFRIQELNFIETDPEIRIGPLTLLSRIAGHPGFSGRLSILREAALAIGDVQ
                     IRNIATVGGNIANASPSADMPPALLSLGAKLRLRKKGRERIVGLEEFCTGPFCTHLEK
                     DELIVEIFIAPVPEGSGAYAWEPKRTAIDETLVGVGAWVLSDADKKTCLRACLALSSV
                     APVPFQAYRAESFLRGQALNPENFRMAGEIAAEESSPRSRADYRRALVSILTEKALER
                     AWRREMER"
     gene            complement(1252..1716)
                     /locus_tag="VEL68_01615"
     CDS             complement(1252..1716)
                     /locus_tag="VEL68_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ34695.1"
                     /translation="MKVQLHLKVNGMEYKKEVEPRRTLLELIREDLELTGTKEGCSLG
                     ECGTCTVLLDGRPIKSCITLAVQANGREVTTIEGLEGSDGTLHPLQQAFIDHGAIQCG
                     FCTPGMVLSAKALLDENPKPTEMEVKRAIAGNLCRCTGYQKIVEAILAVSKS"
     gene            complement(1725..3998)
                     /locus_tag="VEL68_01620"
     CDS             complement(1725..3998)
                     /locus_tag="VEL68_01620"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXZ34696.1"
                     /translation="MADFSVVGTRYGRIDGKDKATGRAKYINDLKFPGMLYGKILHSP
                     YPHARILHIDASRAEKLMGVRAVATAADTPDKKFGGYRSGVKDELIFAKNKVRYVGDE
                     VAAVAATDEETALEALSLIRVEYEELPAVFHPEEAMAPGAPRVHEEVENNVASRAVTL
                     RGDPEKGFREADLILEERFETGLQIHAYLEPVGCIASWDSNRKLTIYAPAQNPSWSRL
                     IYAEALDLPMGKLRVVQTAIGGAFGGKLEQKQYLAAAILAKKARRPVKIVNQRDEEFQ
                     TSMPRVPMVIYLKMGVRKDGMLTAKEHRIFADNGAYTKYASGVLNLGTYRVDGLYRLR
                     NIRNENTLVYTNKLPTSAFRGFGNPQITFAVESMLDQLAEAIGMDALELRLRNAAEAG
                     DVNAHGFKYVSCGLKDCLKKVSEAIDWKEKRGRKNIRRGVGISATSHVCGNRSFFPLF
                     DGATAYARVDEGGKVRVITGEVEVGQGLLTVYAAICAEEMGIPLSEVTVDFPDTDIAP
                     FGLGTWGDRGTFIGGSAVKLAAAEAKKEILEMGAEMLEANPLDLDCREGKVFVKGSPQ
                     KFLPFGEVASAAVYRRGGSPVLGKGTFIPASELADATRYGNISGTYAFGAQAAEVEVN
                     EETGEVQVLKIAAAHDVGRALNPITTEGQIEGATIQGMGYALSEEVRYEKGVMLSPNF
                     LHYRIPTALDVPSMTTFLVETVDPGPFGAKGVAEPAMTPMAPAIANAIYDAVGVRIRT
                     LPITPQKIFEAVEKRKK"
BASE COUNT          883 a         1356 c         1175 g         1014 t
ORIGIN      
        1 cctcatcttt ccatttccct cctccaggct ctttccaaag ctttttccgt gaggatggaa
       61 accagtgccc ttcgataatc tgcccgggac cggggagagg attcttccgc ggcaatctcc
      121 ccggccatgc ggaaattttc aggattcaag gcctggcccc ggaggaaaga ttctgcccga
      181 taggcctgga aaggaactgg cgctaccgag ctcagggcca ggcaggcgcg aaggcaggtt
      241 tttttatcgg catcgctcag gacccacgcc ccgaccccga ccagggtctc atctatggcg
      301 gtacgtttcg gctcccaggc ataggccccg cttccctccg gaacgggagc tataaaaatt
      361 tccacgataa gctcatcctt ttccaaatgg gtacaaaaag gtcccgtgca gaactcttcc
      421 aggcctacga ttctctctct cccttttttc cgcaatcgta atttggcccc cagggacaag
      481 agggccgggg gcatatccgc agaaggagag gcattggcga tattgccgcc caccgtggcg
      541 atgttccgaa tctgaacgtc gccgatggcc aaagccgctt cccgcaggat cgaaagcctt
      601 ccggagaatc cggggtggcc ggcaatacga ctcaagaggg tcaaggggcc gatacggatt
      661 tctggatcgg tctcgataaa attgagttct tgaattcgga aaaggctgat gacggcttcc
      721 gggctcacct ttttctgttt catctggacg accaaatccg taccccccgc caggaccttg
      781 gccctctccc catattcatg aagaaggtcg agtgcctctt ccagggttgc aggttcaaag
      841 taggcgaagg gtttcataat ttcattctgg taaacccccc gctttgccgg gggtcccgcc
      901 tgtgtcggga ttgacaattc agggaagact gagttgtggt ccgggggcaa gggtattgca
      961 cgcaaaaagc atcggccggg tgagcacttg gtccagacgg ttcacccaat ttccgcaaag
     1021 atcggcgggc cctggggcga cccgccggat cgcccctaca agggattgac ccagcacaag
     1081 gttcaggtgt tgtgcccgcc cgttccatgg aatcaaaggc gcctcatggt ttttgcgtgc
     1141 aatacccttg cccccgggcc tggcacctaa tcaaagccac ctttcggggg cgccaatttt
     1201 actcctcttt gagcggttta ctgtttttca taccgccggc cctgccggag gtcatgactt
     1261 gctgaccgct aagatggcct ccacgatctt ttggtagccg gtacagcggc aaaggttgcc
     1321 ggcgatggct cgtttcactt ccatttcggt cggtttggga ttctcgtcga gcagcgcctt
     1381 ggcggataga accatgcctg gcgtacaaaa accgcactgg atggctccgt ggtcgatgaa
     1441 ggcttgctga aggggatgaa gcgttccgtc cgaaccttcc agaccctcga tggtagtcac
     1501 ttcccggcca ttcgcctgga ccgccaaggt aatgcaggac ttgatgggcc tgccgtccaa
     1561 aagcacggtg caggtcccgc attctcccag gctgcaacct tccttggttc cggtcagttc
     1621 caaatcttcc cggatcagct ccagcagggt gcgccggggt tccacttctt tcttatactc
     1681 cattccgttc actttcagat gaagttgaac tttcataagc cctctcactt ctttctcttc
     1741 tccaccgcct caaagatttt ttgcggggta atgggcaggg tccggatgcg aaccccaacg
     1801 gcatcataaa tcgcattggc gatcgccggc gccatggggg tcatagccgg ctcggcgact
     1861 cccttggccc caaagggtcc cgggtccacc gtctccacca gaaaggtagt catcgaaggc
     1921 acatccaaag cggtagggat acggtaatgg agaaagttcg ggctcagcat gactcctttt
     1981 tcatagcgga cttcttccga gagggcatac cccattccct ggatcgtcgc cccctcgatc
     2041 tgcccttcag tggtgatggg gttgagcgcc cgccctacgt catgggccgc ggcgattttc
     2101 aggacttgca cctcccccgt ctcttcgttc acctcgactt ccgcggcctg cgctccaaag
     2161 gcataggtcc ccgatatatt cccgtagcgg gtagcgtctg caagttcgga tgccggaatg
     2221 aaagtccctt tccccaagac cggcgatcct ccccggcggt aaaccgcggc gctggccact
     2281 tctccaaagg ggagaaattt ctggggggag cctttcacaa agaccttccc ctcccggcaa
     2341 tcgaggtcca aaggattggc ctcgagcatt tcggcaccca tttcgaggat ctctttcttg
     2401 gcctcggccg cagccaattt cactgcgctc ccgccgatga aggttccccg gtccccccag
     2461 gtgcccagtc cgaagggggc gatgtccgta tccgggaagt caacggttac ttcgctcaaa
     2521 gggattccca tctcctcggc gcagatggcg gcgtatacgg tgagcaagcc ctgcccgacc
     2581 tcaacttccc cggtaatgac tctgacctta ccgccttcat ccacccgcgc ataggcggta
     2641 gctccgtcaa agagcgggaa aaaggaccgg ttgccgcaga cgtgactggt ggccgagatg
     2701 cccacgcccc ggcggatatt cttccgcccc cttttctctt tccagtcgat agcctcggag
     2761 accttcttca aacagtcctt cagcccgcag ctcacgtatt tgaacccgtg agcgtttacg
     2821 tccccggctt cggcggcatt gcgcaggcgc aattccaggg catccatgcc aatcgcttca
     2881 gccaattggt ccagcatgga ctccacggca aaggtgattt gcggattgcc aaagccccgg
     2941 aaggccgaag taggcaactt gttggtatag accagggtat tttcgttgcg gatattgcgg
     3001 agccggtaaa gcccgtccac ccggtaggtc cctaagttca ggacgccgga agcatacttg
     3061 gtgtaggcac cgttgtcggc aaagatgcgg tgctccttgg cggtgagcat tccatccttc
     3121 cggaccccca tcttcaagta aatgaccatg ggaacccggg gcatggaagt ctggaactct
     3181 tcgtcccgct ggtttacaat tttcaccggc cttctggctt tcttcgccag gatcgctgca
     3241 gccaggtatt gcttctgctc caatttcccg ccgaaggctc caccgatggc cgtctgtacg
     3301 acccggagtt tccccatggg aaggtccaag gcttcggcat agatgagacg cgaccaagaa
     3361 gggttctggg caggggcgta gatcgtcaat ttcctgttgg aatcccaaga ggcaatgcaa
     3421 ccgaccggct ccaggtaggc atggatctga agtcccgttt caaaccgttc ctccaggatc
     3481 aggtcggcct ctcgaaatcc cttttccggg tcccctcgca aagtaaccgc gcgggaggct
     3541 acgttgtttt ccacctcttc atgaactctc ggagcgcccg gcgccatcgc ttcttcggga
     3601 tgaaacaccg caggcaattc ttcgtattcc acccggatca aggataaggc ctccagggcg
     3661 gtctcctcgt cagtagcggc aacggcggcc acctcgtccc ccacgtatcg caccttattc
     3721 ttggcaaaga tcagttcatc cttcaccccg gagcggtaac ccccaaattt tttatccggt
     3781 gtatccgcag ccgtggccac ggcccgcacg cccatcagct tttcggcccg gctcgcgtct
     3841 atgtgaagaa tccgggcatg agggtaaggg ctatggagaa tcttgccata aagcatcccg
     3901 ggaaatttca ggtcgttgat gtatttggcc cgccccgtgg ctttgtcctt gccgtcgatc
     3961 cgaccatagc gggtccccac tacggaaaaa tcagccattg cccctccttg ccaggaaagc
     4021 ccatcctaag cagtccaaat tgaaaagatg tatactttta aaaaattcct ctaaacgcga
     4081 tgctcttcac tcaagttctc accgttcatc ctgagccctt cgattcgccg ttcgtcctga
     4141 gcttgtcgaa ggacgaacgg cacgctcagg ccttgcttgt cgaagggtac atcgctaggc
     4201 cgttcatgct tcgacaagct cagcacgaac ggcttagtct tcagcatcca tacaattatg
     4261 gactgatcag taagcctgcc aggccttctt taaagcctgg aagaccaggc tggcagttat
     4321 ttctctcgta aagcggtcca tccgtcccga tttggaaatt tgaagagcct cttggctggc
     4381 ctgccgggcg cagtcctgca gcatcggttc ggtgatcctc tgcccaat
//
LOCUS       DASPRR010000044        22611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2270876, whole genome shotgun sequence.
ACCESSION   DASPRR010000044 DASPRR010000000
VERSION     DASPRR010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 22611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22611)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22611
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2270876"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VEL68_01625"
     CDS             <1..897
                     /locus_tag="VEL68_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ34697.1"
                     /translation="LVRSAAAAALATYSSPQAQEALLEASGDDFRLVRVNAAMALSHF
                     PGLPPNKDDQKKLERATGEYLASLLSRPDQWSSHYNLGNYHLNRGDLALAMGAYETAL
                     KMEPRSIPALVNASMAQARMGNQEKAESFLRKALEEDPGNAAANFNMGLLKAEQKNSA
                     EAEKHLRAALKTDPQMDAAAYNLGVLLAMDRPEEGLQFLRKANELRPDIPKYGQALAF
                     YLGQKGDQTKAIQALQETISRQPGNGDAYLMLGEIYLRQGKPERASAVYVQALRNKAL
                     SDSDRERIGAKLKELAAAKLRK"
     gene            complement(1025..2236)
                     /locus_tag="VEL68_01630"
     CDS             complement(1025..2236)
                     /locus_tag="VEL68_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXZ34698.1"
                     /translation="MDTFIARQPIFDSRQRVFGYELLFRSSLENVFKHSNPDQATSKV
                     ISDAFFLFNFSTLTEGKRAFINLTREILVKEYIFLIPKHFVVVEILESVDPDSEVIAA
                     CKKMKQAGYLLAMDDFVYKERLTPLLEIADFIKVDFLTTSESERRALAKEFAPLGIRL
                     LAEKVETPKDFLEALEIGYSYFQGYFFCKPVVIKGKDIPGSKLHYFQVLQEIHHRQMD
                     FRRLEQIIKQDVSLSYKLLRYINSAYFGLRNRINSIKQALLLLGQAEIKKWLSLITLA
                     GMGTDKPGELVVHTITRAKFCESLAPHAGLLRRADDLFLVGMLSLMDAFLDRPISDLL
                     IEVPINDEIKGAILEKENRLGEVYDYVLSYEKGDWNRLNEQQMKLGIHENYPPQLYLN
                     ALKWGKEAFYH"
     gene            complement(2765..3571)
                     /locus_tag="VEL68_01635"
     CDS             complement(2765..3571)
                     /locus_tag="VEL68_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXZ34699.1"
                     /translation="MPSDPLRNITIVLVRPQYAGNIGAVCRAMKNMGLTRLNLVSPEQ
                     DPLSAEARMMATSAKDILQKAKIFPSLEDALQGFRWIAGTSARKGINRGPFISPREIC
                     PEIIQHARSIPVAILFGPEDRGLTNRELDPCHALISIPTHPGLSSLNLAQAVILLSYE
                     LYLASLYEIRNEECGMQNEKRIRREEARSSSGLLPLLAEFQKVEKMYAHLEQLLLRIG
                     FLDSNNPKRIMHTLRRIFGRANLCDRDVAILRGIFRQLEWYATHARQKKD"
     gene            complement(3553..4344)
                     /locus_tag="VEL68_01640"
     CDS             complement(3553..4344)
                     /locus_tag="VEL68_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXZ34700.1"
                     /translation="MKVFDGDTILVRIQGHEDFVRLREIDAPEMANRKLQGQEPWGSK
                     AREFALSRLRDRKVRLETDDREERDAYGRLLAYVFVGDSLLNREMVQSGNAFFYRAPI
                     RGKYAAQLEEAEEAARDKGVGVWDRKKGLKEKPRDFRARLRRNEGLFSPSSRTTGAEK
                     KGRSIQEYAVPWDKIVGNKRTMVYHLPGSPSAKRVSLKNRVIFNSAEEAEKAGFRKAN
                     QMRYAEFGMRNYEKQNELGILKIEALPPTVYANLPSPSYPCRAIL"
     gene            complement(4577..5140)
                     /locus_tag="VEL68_01645"
     CDS             complement(4577..5140)
                     /locus_tag="VEL68_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34701.1"
                     /translation="MSQMREPEEPARFWEFLFQDLQEELTQLRGEFLIFLLHLPLRLG
                     FLFWGWRNYLFLFLLLPILLIACEEEESEGEPEKHSQVFEFNEKYIIRGIANLLKEKG
                     YADPKVDIEKGKVETDYIPQGNLRVKVETTVKKLDRREREVVLLITTEKKTQKGWKAI
                     KMLDKAHYDRFFDELEMQIYREMAKGD"
     gene            complement(5065..5229)
                     /locus_tag="VEL68_01650"
     CDS             complement(5065..5229)
                     /locus_tag="VEL68_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HXZ34702.1"
                     /translation="MPNYEFQCQKCNKKYSLFLSIKDREERKYKCPKCGSRKSQPVFG
                     NFYSKTSKKS"
     gene            complement(5631..6338)
                     /locus_tag="VEL68_01655"
     CDS             complement(5631..6338)
                     /locus_tag="VEL68_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34703.1"
                     /translation="MGRRPFPSLVLVGTVHRDPQGKTKLLRLLQREQPSAVSVEVSPY
                     ARIFRNQKAAAFRAALRENLRRIHQEEGLAWRGMLSHSAIQGIFLLLKEPYEWRAAKA
                     YGEETNCRIQDIDLSEYSEERLFHLPELVSRENLRTLLSLSFPGLNEQVRNHYGRARF
                     LFSHPPSVWLESRELRERESIMAEKIRGMVFQEVGGKIVHIGGWEHLLEFSGKSSLYS
                     LLQDLHPRRVLLADGED"
     gene            6485..7702
                     /locus_tag="VEL68_01660"
     CDS             6485..7702
                     /locus_tag="VEL68_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ34704.1"
                     /translation="MISSFRKELILVALAGTIAVMGLGFIIPLFPVYVSQKGATNFQL
                     GLIVSGFTISQFLVQPFFGGLSDTYGRKAFMVGGLACYAIVASLYVLAESLTEVFLIR
                     LFHGVGAGMIWPALSAFIIDESPVDKRGETIGFISAMEMFGFAAGPLVGGGLYSLGGM
                     SLPFWGCSLLAFAGMALIWGLVQEKPSPHHSEKVGFFERYGFSSLRIPDIRLLCLIGF
                     GESFAWGLIITLLPVIASNLGVPPERIGWLFSSYFIFYILLQWPVGKWSDRQGRKKPI
                     ILGMSIYTLSILILSQGGDLFHFLLVLAIAGAGLGIYSPSVRVAVADLTSEKVRGASL
                     GIFITTRMLGFFLGPNLSGFMADRFGSGFPFLTAAVFLGVGIWASFRLSAELSKTREL
                     ASIPAGGAGKNTR"
     gene            7758..10739
                     /gene="purL"
                     /locus_tag="VEL68_01665"
     CDS             7758..10739
                     /gene="purL"
                     /locus_tag="VEL68_01665"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXZ34705.1"
                     /translation="MAYRVEVGLKRNIRDARGEKIKRRIMDDLKIPIEAVRTVDVYTV
                     DASLSDEDMEKVATGPFLDPIIQDYSVGKPLEQAGFDWAIEVGYKPGVTDNVGRTARE
                     AVEILLQRKLNPGDGVYTSVLYFLKGTLARNQAESIATGLLANTLIQRFEIKDRDSWD
                     PAVGMGVTVPKVSAKNQVRVEELNLQVSDEELLRISSRRVLALSLKEMKALQQYAKDP
                     SRLAERKKVGLGEHLTDCEAEALAQTWSEHCKHKIFNGLIHYIDEKGKARRIDSLFDS
                     YIKRSTDEIRKALGEKDWCVSVFKDNAGVMRFNRDWNLVFKVETHNSPSALDPYGGAL
                     TGIVGVNRDPFGTGKGAKLIFNVDTFCFAPPDFSKPLPPRLLHPKRIYEGVREGVEHG
                     GNKSGIPTVNGSIVFDDRYAGKPLVYCGTGGIMPRLIRGEPSHQKKAEPGDFIVMTGG
                     RIGKDGIHGATFSSEELHEESPTSAVQIGDPITQKRMTDFLLRARDAGLYHAITDNGA
                     GGLSSSVGEMARDCNGCEMSLERAPLKYAGLDPWETLLSEAQERMTLAVPPDKIDRFM
                     EMAGLMEVEATILGKFTDTGKFHVLYDGKTVAYLDMEFLHNGYPKMELRAKWEQKEHP
                     EPQSPDPKDLTSTLKQILSRYNVCSKESVVRQYDHEVQAGSAVKPMVGAFNDGPGDAS
                     VLRPILDSWEGVVVSSGICPRYSDIDTYHMMACAIDEGIRNNLAVGGNLKQMAGLDNF
                     CWCDPIQSEKTPDGEYKLAQLVRANQALYEYTKAFGVPCISGKDSMKNDYLIGSMKIS
                     IPPTVLFSVVSVVPDVRKCVTMDAKRAEDMVYVLGETRAEMGASEYYALHGFIGNQVP
                     RVDAQKARNLFQSLSAAMEKGLVASCHDCSDGGLGVALAEVAFAGGLGIEVDLRRVPA
                     SGMDRNDFVLFSESQSRFVVTVHPENQKAFENSLQGNVFARVGRVKAGQDLAITGLQG
                     QVVVRADIDELKEAWKKPLMW"
     gene            10828..11649
                     /locus_tag="VEL68_01670"
     CDS             10828..11649
                     /locus_tag="VEL68_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXZ34706.1"
                     /translation="MKKVKALILAGNGINCEMEMAHGCRLGGADVADIVYIYDLLSGE
                     VRLDDYHFLNLPGGFLDGDDLGSAKAMAHRLKYGRIAGSGEPLEKGILRFIREGKLIL
                     GVCNGFQLMVKSGLIPGLGKIYGHQTVTLTFNDSGRFEDRWVYLKANPQSPSIFTKGL
                     KGLYLPVRHGEGKFIPRDEEVLAELARKNLIALQYSDPDYQNPTMEYPLNPNGAVSAI
                     AGVCDETGRLFGLMPHPEAYLYFVNHPRWTREKVPEEGMGLALFRNAVDYIRKNL"
     gene            complement(11733..12695)
                     /locus_tag="VEL68_01675"
     CDS             complement(11733..12695)
                     /locus_tag="VEL68_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34707.1"
                     /translation="MKKIFVSMISLALVLLPAVAFPAADFPKGPITYIIPFNPGGQSD
                     VEARMQQPHLEKNLGVPIVINYVPGAGGGLAWTKFSQAKPDGYSVCGINIPHIILQPL
                     SQSDVAFQTEDLKPLCIFESTPIGLAVKKDSKINGVKEFIEFARANPNKITIGMSGKY
                     SGHHMASLLFMKMNNIKLTLVTFTGAAPQVTALLGGHVEAIWGNSSDLVAQAGQMKIL
                     AIGTEKRMNVLPNVPTFQELGHKFYPRIDRGVAVPKNTPPDITTRLEKAFLDTVNKEE
                     YQSKIISAGFVPLILNGEQSIKYIRGETETIKKLLAEHDLLLKK"
     gene            complement(12876..13838)
                     /locus_tag="VEL68_01680"
     CDS             complement(12876..13838)
                     /locus_tag="VEL68_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34708.1"
                     /translation="MKKWQAACLIFGFLLAISSSVANAQDFPKGPINYLNPFNPGGEV
                     DIAARAMQPYLEKNLGVPFVINYLPGAGGGLCWSKLAAAKPDGYTIGGFSIPHIILQP
                     IFMKDVTFKTDDFVILSIVEYTPIGLGVKKDFKANTFKEFIDYCKANPGKVSCGGVGK
                     FTGHHFATLQFMKLAGVKVNYVTFTGTSQVQTALLGGHIDAAFTNSSVLTTSKDQIKV
                     LAIGSPKRMVQLPEIPTFEELGYKMYPRIARGAMGPKAIPKDIQKRLEKAFLETISKP
                     EFISKMEQAGFVPQVMGIEESHKYLDMEVKEHMKLAQEFDLKKQ"
     gene            complement(13874..15391)
                     /locus_tag="VEL68_01685"
     CDS             complement(13874..15391)
                     /locus_tag="VEL68_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ34709.1"
                     /translation="MDILSHILLGAQVLFTPTNLVFCFLGVLMGTLVGVLPGLGPTAA
                     IALLLPNTFHVPPVTAIIMLAGIYYGAMYGGSTTSILVNIPGEAASVITCLDGYQMAL
                     KGRAGPALGIAAFGSFIAGTISVLGLMLVAPPLAKFALAFGPPEFFSLMLMGIVVLIY
                     LASGSILKALMIAVFGLLLGTIGMDAISGTQRLTFGVLELADGIGLIPAIMGLFGVAE
                     VISNVEEEIKREIIPAKVKDLLPNLQDWKDSVWPIVRGSFLGFFIGILPGPAPVISAF
                     TSYAVEKKLSRHPEKFGTGAIEGVAGPESANNSATGGAFIPLFTLGIPANSVIAILLG
                     AFMIHGIQPGPMLISKYPDLFWGTIMSMYLGNVMLLILNLPLIGLWVKVLKVPYPILF
                     PLILLFCLVGVFSLNYSNVEVSLMIGFGVFGYLARKFQFEMAPLVLAIVIGPMMENNL
                     RLALTISQGDPWIFIQKPISAVFIVISLVLLISPFIPWLGKRRQKLREKVEGEEV"
     gene            complement(15399..15854)
                     /locus_tag="VEL68_01690"
     CDS             complement(15399..15854)
                     /locus_tag="VEL68_01690"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ34710.1"
                     /translation="MRYNRISAIFFLIVGLFFSIYARSVEIGAWNEPGPGFLPFWGGI
                     VLTAMSIALLMRTFWGKLPAITHSFFSRSDSWKRVTATFAALIAYNLLLTTLGFTLMT
                     FLFVGFLVRFIFPQTWTRSLVIAVLSSLAAQLLFVNFLETQLPKGFLGF"
     gene            complement(16235..17074)
                     /locus_tag="VEL68_01695"
     CDS             complement(16235..17074)
                     /locus_tag="VEL68_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ34711.1"
                     /translation="MEPIIGRYMKVRIMGDDCRVYFEETGQGIPLVCLHTAGADSRQF
                     RHLMCDETVTNKFRVIAFDLPWHGKSYPPAGWEKREYRLTTELYVATIRAFCAALGLE
                     HPVALGCSIGGRIILQLAHAHGSEFRALIGIESADFQQPWYDTNWLHRGDVHGGEVCA
                     ALISGLIAPQSPDEHRHETLWQYMQGGPGVFRGDLYFYRVDADLRGKLDGINTAACPL
                     YLLTGEYDFSCQPEDTLRTANTIPGVKVMIMKELGHFPMSENPAQFRKYIIPVLEEIR
                     RKG"
     gene            complement(17086..17508)
                     /locus_tag="VEL68_01700"
     CDS             complement(17086..17508)
                     /locus_tag="VEL68_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34712.1"
                     /translation="MPEDSKNSPAGLEQALSARFERVIPEAVNSDRRLVWRGRNLTVD
                     CLVQIGTVPFLLRIEEGSIRECRKGLPLLCSWVFAVRGSAQAWAALWQDPPPPGWHDI
                     FALTKRGEMALEGNLHPLMANLQYFKDVVALPRKGGSR"
     gene            complement(17660..18559)
                     /locus_tag="VEL68_01705"
     CDS             complement(17660..18559)
                     /locus_tag="VEL68_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXZ34713.1"
                     /translation="MENIGFIGLGAMGGPMAKNLVKKGFSLMVFDIVAERMGPLKAAG
                     AQTAASCREAAEKCPVVITILPASAHVREAVLGKGGVVEGIQRESVLIDMSTIDPLTT
                     RDIAKILGEKGVEMLDAPVARGVPAAVAGTLVIYAGGKKEVFERHRPILAAMGTDIYH
                     VGDIGSGEVVKIVNNLMVAVTTCALAEAMVLGVKAGVKPDILFEALSSGSGNSFVLQN
                     HYKNNVMKGKFEEGVFPIDYILKDLDLALSTGSKLRIPLHFAALSAQQYISAGAGGES
                     KKYHPAVIRPLEKLAGVEVRSEK"
     gene            complement(18668..19372)
                     /locus_tag="VEL68_01710"
     CDS             complement(18668..19372)
                     /locus_tag="VEL68_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34714.1"
                     /translation="MLKLVKVRVDYGQTQALKGIHLSAKEGSIVALVGPNAAGKTTTL
                     KAITVLKPITSGEIYLRGKRIDRLDTSEVIRQGIALCPEGRRVFSELTAQENLRLGAY
                     LRRNRQEVREDTERVYGYFPALKKREKQVSGTLSGGEQQMLAIGRALMAKPQYLLLDE
                     PSLGLAPLVVKAIAAIIKQINGAGVSIILVEQNARMALRLAEYAYVLESGEVALEGKA
                     EDLLHNDYVRKAYLGG"
     gene            complement(19365..20138)
                     /locus_tag="VEL68_01715"
     CDS             complement(19365..20138)
                     /locus_tag="VEL68_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34715.1"
                     /translation="MTPILQVEKLSKQFGGLMAVDQATFSVQPGEILGTIGPNGAGKT
                     TLLNLISNMYAPHSGTVRYKGESIVGYPPYRIAKMGLVRTFQSTVSYGEATVWENMMR
                     AQLGYHFLGLWPEFLINRAVRERKKNQTEKAETLLRFLGLEKMRGQAAKALPYGYQKL
                     LGLAIALAGDPQLLMLDEPAAGLNHEERVHLMSAIQKIQENGVTVILVEHDMKVIMGL
                     CHRILVLNYGQMIAMGTPPEIQRNEEVIKAYLGEDTEIA"
     gene            complement(20135..21157)
                     /locus_tag="VEL68_01720"
     CDS             complement(20135..21157)
                     /locus_tag="VEL68_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34716.1"
                     /translation="MGTTGPDPMQEEGKVIPKSKSYALLALSVFLFFIPVLWADEFYL
                     NVIISIYLNIILAVSLNMVIKTGKLSIAHAAFMGIGGYTSALTVMRLGLPYLLALPLS
                     GLMGACIALLVGRLLLKLQGVYFVLVTFAFGEVVRLFFVNLVEPFGGPTGIINIPPPR
                     IALFPGTAFTLLSKVSYYYLVLGVMLMALFIIRRIYQSSIGKAMDCTRETEHLAEFTG
                     IDTFQHKMFAFAGGGFFAGLAGSLFAHYYRFISPESFTFWESVNLIVINVVGGMGGIA
                     GPILGSIFLVPLPEFLRGFVEYQRVLYGLILILTLWFMPTGMLGLLNKALDRMKSRKP
                     GKEETP"
     gene            complement(21172..22044)
                     /locus_tag="VEL68_01725"
     CDS             complement(21172..22044)
                     /locus_tag="VEL68_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34717.1"
                     /translation="MGETLLPQVIINGVTLGTNYILMALGFTLIFGVLRIVNFAHGEF
                     YMLGAFFVLTAILKFEVNYLLAAILAVLFIGLLGYLTEEVIFRRFRDKELEGMIIALG
                     LSILLQNLGLVAWGPYDLSIPPVFTGVLKIGTLFYPMERLMVVFISFLLMAGFYLFLK
                     KTRLGLAIQAIAQDTEIARVQGMRINRLYPLAFAVGTGLAAAAGAVIGPLFQLNPWMG
                     IMPQVKAFIVVILGGIGSIPGAFLGGLILGVAESFVSTYASKALADLLGFVLVIGILL
                     FRPSGLFGRSQVSR"
     gene            complement(22112..>22611)
                     /locus_tag="VEL68_01730"
     CDS             complement(22112..>22611)
                     /locus_tag="VEL68_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34718.1"
                     /translation="KPDVLNLDGSNPGDCGLIVKQARQMGYKGMIIKVGGPGTPEMLK
                     VAGKDFMEGFIYYSPINPDDPKIQEINKKYNAKFPPPMNGFTPQFYDGTLWLFEAIKK
                     AGTLTDTEKVRATLESLSFNGVYTGAMSWTGKADYGIDHQVKFPFYVMVVKDGKEVVL
                     GKATP"
BASE COUNT         5140 a         6165 c         6097 g         5209 t
ORIGIN      
        1 ctggttcgtt ccgctgctgc cgcagccttg gccacatatt cgtccccgca ggcccaggag
       61 gcgctcctcg aggcctcggg ggacgacttt cggctggtgc gggtgaatgc ggccatggcc
      121 ctttcccact tcccgggcct gccccccaat aaagacgacc agaagaagtt agaaagagcc
      181 acgggggaat atttggcctc tctcctatcc cggccggacc aatggtcttc ccactacaac
      241 ctgggaaatt atcatctcaa ccgtggggat ttagccctag ccatgggggc ttatgagaca
      301 gctttgaaga tggagccgag aagcattccg gcgctggtga atgcctccat ggcccaggcc
      361 cggatgggaa accaggaaaa ggcggagagt ttcctgcgca aggccctgga ggaggatccg
      421 gggaacgcag cggcgaactt caacatgggg ctcttgaagg cggaacagaa gaattctgcg
      481 gaggcggaga agcacctgcg ggcggcgctc aaaaccgacc cgcagatgga cgcggcggcc
      541 tataacctgg gggtcctgct ggctatggac cgcccggaag agggtcttca gtttttgcga
      601 aaagccaacg agctccgtcc cgatattccg aaatacgggc aggcgctggc tttttatctg
      661 gggcagaagg gagaccaaac caaagccatc caggccctcc aggaaactat ttcccgccag
      721 ccgggcaacg gtgatgctta cttgatgctg ggggagattt acctcaggca gggcaaaccg
      781 gaaagggcat cagcggttta cgttcaggcc ctgagaaaca aagccctttc tgattccgat
      841 cgggagcgga tcggagccaa gctgaaggag cttgccgccg ctaagctccg gaaatagggt
      901 ttccctttcc ccgaggcttc cggagttagg gtagcttggt gaccaatggt acatttttta
      961 gtgtcgacgg ggcttaattc attattttca atggccaact taatatgaca cgggggctgg
     1021 caaattaatg ataaaacgcc tctttccccc attttagggc gtttaaataa agctgaggag
     1081 ggtaattctc atgtatgccc aatttcatct gttgctcatt tagtcgattc caatcacctt
     1141 tttcgtatga aagaacataa tcataaacct cccccagcct gttctctttc tctaaaatag
     1201 ctcctttaat ttcatcattg atcggtacct ctattaataa atcggaaatg ggacgatcca
     1261 agaatgcgtc cattaaggaa agcatgccca ccaaaaagag atcatcggcc cgccttaaaa
     1321 gacccgcatg gggagccaaa gattcacaaa atttggctcg cgtgatcgta tggacaacga
     1381 gttcccctgg cttatccgtt cccattccag ccaaggtaat gagcgaaagc cattttttta
     1441 tttctgcttg ccctaaaagc aataatgctt gcttgatgga attgatcctg tttctcaacc
     1501 cgaagtaagc cgaattgatg taccggagaa gcttatacga gagggagaca tcctgtttaa
     1561 tgatttgttc cagtcttcta aaatccattt gcctatgatg gatttcctgg agaacctgaa
     1621 agtaatgaag tttagaccct ggaatgtcct tcccttttat caccaccggt ttgcagaaaa
     1681 agtatccctg gaagtaacta taccctatct ccaaggcctc taaaaaatcc tttggcgttt
     1741 ccaccttttc cgccaaaagt cgaattccca agggggcaaa ttccttggcc agagcccttc
     1801 tttcactctc gcttgtggtt aaaaaatcca cttttatgaa atcggctatt tctaatagag
     1861 gggttaatct ctccttataa acgaaatcat ccatggccag gagatagccc gcttgcttca
     1921 tttttttaca ggccgctatc acttcggagt ctgggtctac gctttccaga atttccacta
     1981 caacgaaatg tttgggaatc aaaaaaatgt attctttgac cagaatttct cgagttaaat
     2041 tgatgaaggc tcttttccct tctgtgagcg tggagaagtt gaaaaggaaa aaggcatcgg
     2101 agatcacctt ggaggtggct tgatccgggt tcgaatgctt gaaaacattt tccaggctcg
     2161 aacggaaaag tagctcgtat ccaaaaactc tctgccggga atcaaagatt ggctgtctgg
     2221 cgatgaacgt gtccaagatg aatctttcct tctgtagcat gtatcggtca tttgaggcat
     2281 ttattgaatt ctctttttac ggggaaaggg atattacaag ggggttattg atatggatgg
     2341 attaaagaag gaaatccggg aagatagggc aggagggggc gcctgctttt ggcatgaccg
     2401 cccccaaaaa tctttttggg tgccgccttg gacggatcgg aggacccatt cttgaataat
     2461 ccaaggcagg actgggttcg ttgttaaagg gtccccctgg taaaactctt gattttctcc
     2521 gaccagccgc gcccgttctg tgatcgcctt ctggaagatc agttttgaac tggaaataag
     2581 gttggtgttt tcggccatca aatatctttc tgccggtagg caatactcag ggtcgatttc
     2641 gagccgatgc tgttccgtcc tgttctgaga ccggcgatta tcgttgtgcc cgatccgcag
     2701 aacggatcaa gcagtgggtc gccggaaata cctccccttt attctgagtt ctgccttctg
     2761 gtttctaatc cttcttctgt cttgcgtgcg tggcatacca ttcgagctgg cggaaaattc
     2821 cgcgcaggat cgctacatcg cggtcgcaga ggtttgccct tccaaagatt ctccgcaggg
     2881 tatgcatgat tctcttggga ttgttagaat ccaaaaagcc gatccgcagg aggagttgct
     2941 ccaggtgggc atacatcttc tcaaccttct ggaactcagc tagcaggggt agcagcccgg
     3001 aagacgaacg agcctcctcc ctccgtattc ttttctcatt ttgcattccg cattcttcat
     3061 tccgtatttc atacaggctg gccagataca attcataaga gagcagaatc acggcctggg
     3121 ccagattcaa cgaggataaa ccagggtgag tgggaatcga gataagggca tggcaggggt
     3181 cgagttctcg attggtcagt cctcggtcct ccggcccgaa aagaatggcc acggggatgg
     3241 atcgggcgtg ttggatgatt tccggacaga tttcccgggg agagatgaaa ggcccacggt
     3301 ttatcccttt acgggcggaa gtgccggcaa tccaccggaa gccctggagg gcatcctcca
     3361 gagagggaaa aattttggcc ttctgcaaaa tatccttggc cgaggtagcc atcatcctcg
     3421 cttccgcaga tagtgggtcc tgctccggag acaccaggtt caatcgggtg agacccatgt
     3481 ttttcatggc ccggcaaacc gccccgatat ttccggcata ctggggcctg accagcacaa
     3541 tggtgatgtt ccttagagga tcgctcggca tgggtaggaa ggggaaggca ggttggcgta
     3601 tacggttggt ggcaatgcct cgattttcag aattcccaat tcattttgct tttcataatt
     3661 ccgcattccg aattccgcat atcgcatttg attggccttc ctgaaccctg ctttctcggc
     3721 ctcttcagcg gagttgaaga tgacccggtt cttcaggctc acccgcttgg ccgaggggct
     3781 tcccggaagg tggtacacca tggttctttt atttcccacg attttatccc agggcacagc
     3841 atactcctgg atggagcggc ctttcttttc tgcacccgtg gttcggctcg acggagaaaa
     3901 aagaccctca ttccgccgga ggcgggcccg gaaatcccgg ggcttttctt tcagcccctt
     3961 cttccggtcc caaactccga cccccttgtc ccgcgcggct tcctccgctt cctccaactg
     4021 ggcggcatac tttccccgga tcggggcccg gtagaaaaag gcattccccg actgcaccat
     4081 ctcccggttg aggagagaat ctccgacaaa gacgtaagcg aggagccggc cataggcatc
     4141 gcgttcttcc cggtcatcgg tctccagccg aactttccgg tccctcagcc gggagagggc
     4201 gaactccctg gctttgcttc cccacggctc ctgcccttga agtttacgat tcgccatctc
     4261 cggcgcatcg atctcccgca ggcggacgaa atcctcatgc ccctggatgc gcaccaggat
     4321 ggtgtcgcca tcaaatacct tcatgacttg gccctgatag gtcttagctg aggcaaggga
     4381 agaaaagaaa aggcaaagaa gaaggaaaaa aagcagcccc cggatgaaag tcttcataaa
     4441 gaaacctgag gctcatttac gggcgggcac ggcggacctt ccctacccat ctcaaaatcc
     4501 ttgtttctcc atgttgatct gggccctgtg atcttttccg tcgatatttg ctaattttgg
     4561 gcactctagg cacctcttaa tcccctttgg ccatctcccg gtatatctgc atttccaatt
     4621 catcgaagaa ccggtcatag tgggccttgt ccaacatttt gatcgctttc cacccttttt
     4681 gggttttttt ctcagtagtg atcaacagga ctacttccct ttctctccgg tcgagcttct
     4741 tgaccgtagt ttccactttt accctcaggt ttccctgtgg gatgtaatcg gtctctactt
     4801 tgcccttctc gatatccact ttggggtctg cgtaaccttt ctccttgagc aggttggcaa
     4861 tcccccggat tatatatttc tcattgaatt cgaagacctg ggaatgcttc tccggctctc
     4921 cttccgattc ttcttcttcg caggcgatca agagaatggg gaggaggaga aagaggaaaa
     4981 ggtaattcct ccatccccag aaaaggaatc ctagcctaag gggcagatgt aagagaaaaa
     5041 taaggaattc tcctctgagc tgggttaact cttcttggag gtcttggaat agaaattccc
     5101 aaaaacgggc tggctcttcc ggctcccgca tttgggacac ttgtactttc tctcctctcg
     5161 gtcttttatt gaaaggaaca gggaatactt cttgttgcat ttctggcatt ggaactcata
     5221 atttggcatg ggcgtacctc ctttaaagat ttaatcaatt gactacggtt tcaaaacgga
     5281 caccagacct ttatcttcgg actccctggc ccggaaaccc ttttcctttg cgggttttaa
     5341 atgagggagt gttttagatt gttgataata gggcgtttcc atcctcttgc gtctcgggaa
     5401 ccctgtattt gcagggttct tgtgctaact atttattttt atttaatatt ttattggtac
     5461 aaaaatgggc agtttacaca aactctcgag attctatgag attatgcctt tctatccaaa
     5521 acttttccac aactttttca caaattttgt ggataatttc cgggctctaa ttttcactct
     5581 ttttcgggaa aggatgtccg cctttccagc ggggttaact tccctttccc tcaatcctct
     5641 ccatccgcca aaagaaccct ccgaggatgt aaatcctgca acaaactata aagagaagat
     5701 ttcccagaga attccaaaag atgctcccac ccaccgatat gaacgatttt ccctccgact
     5761 tcttgaaaga ccattccccg aatcttttcc gccatgatgg attctctttc cctcagctct
     5821 cggctctcaa gccagacgga aggaggatgg gagaaaagga atcgagcacg cccataatgg
     5881 tttctcacct gttcgttcag ccctggaaaa gaaagggaaa gcagggtacg caaattctcc
     5941 cgggagacga gctcaggcag atggaagagt ctttcttccg aatactcgga aaggtcgatg
     6001 tcctgaatcc tgcaattcgt ttcctccccg taggccttgg ccgccctcca ttcgtagggt
     6061 tctttcagaa gcaggaagat tccctggatg gcgctgtggg acagcatccc ccgccaggca
     6121 agaccctctt cctgatgaat tcgtcgaaga ttttcgcgca aagcggcacg aaaagccgct
     6181 gccttctggt ttcggaagat tcgggcgtag gggctgacct ccacggagac ggccgatggc
     6241 tgctcccgct ggagcaatcg caatagctta gtcttccctt gcggatcgcg atggactgtt
     6301 cctaccaaca ccagggaagg aaaaggtctc ctacccatac ccatccatcg gttgaccttt
     6361 tttgaaacct tgtggtaaag tcaaaccgtc ttcccgtaaa gctcttcaga atctcccgca
     6421 aagagcccgg accgaacgaa aaatattttt acccaattag accgaaaatt caagactttt
     6481 ccttatgatc tcatctttcc ggaaagagtt gatcctggtt gccctggctg ggacgattgc
     6541 cgttatgggg ttaggtttca ttatccccct ctttcctgtt tatgtctctc aaaaaggagc
     6601 caccaatttc caattgggct tgattgtttc cggcttcacc atcagccagt tcctcgtcca
     6661 gccctttttc ggcggactct ctgataccta tgggcggaaa gctttcatgg tgggaggctt
     6721 ggcctgctat gcgattgtgg cctctctcta cgttctggcg gaaagcctca ctgaggtttt
     6781 cctcatccgg ctgtttcatg gggtgggggc aggaatgatc tggccggccc tgtcggcctt
     6841 tatcatcgac gaatcacccg tggataaacg gggagaaacc atcgggttca tctccgcgat
     6901 ggaaatgttc ggttttgccg caggccccct cgtggggggg gggttgtatt cccttggagg
     6961 tatgagcctg cctttctggg gctgttctct cctggctttt gcaggaatgg ccttgatctg
     7021 gggattggtt caagaaaagc catcccccca tcattccgaa aaggttgggt tctttgaaag
     7081 gtacgggttt tcctccttgc gcattcccga cattcggctc ctctgcctca tcggcttcgg
     7141 cgaatctttt gcctggggtt tgatcatcac ccttctcccg gtcatcgctt caaacctcgg
     7201 agtccctccg gagagaatcg ggtggctctt cagctcttac ttcatctttt atattctcct
     7261 tcaatggccc gtggggaaat ggtcggaccg gcagggaaga aagaagccca tcatcttggg
     7321 catgagcatc tacaccctgt ccatcctcat tctttcccag gggggagatt tattccattt
     7381 ccttctggtc ctggccattg ccggggcggg actgggaatc tattcccctt ccgtgcgcgt
     7441 cgcggtggcc gacctgacat cggaaaaggt ccggggagcg agtttgggca tttttatcac
     7501 cacccggatg ctggggttct tcctgggccc caacctgagt gggttcatgg ccgaccgttt
     7561 cgggagtgga tttccatttt tgaccgcggc ggtatttttg ggtgtcggga tctgggcatc
     7621 gttcaggctg tcggcggagc tttcaaaaac ccgcgaactc gcctcgattc cggcaggtgg
     7681 tgccgggaag aacacccgct agattctcaa agcattcttt ttgcattcct ttgaaggaaa
     7741 tgaaaagagg tgaaagaatg gcctatcgcg tagaggtcgg attaaaacgc aatattcgag
     7801 atgccagggg ggagaagatc aagcgacgga ttatggacga tctgaagatt cccatagagg
     7861 cggtccgaac cgtggacgtc tacacggtgg acgcctccct atccgatgag gatatggaaa
     7921 aggtggccac gggacctttt ctggacccca tcatccagga ttattccgtc ggcaaacccc
     7981 tggagcaagc aggttttgac tgggccatcg aggtgggcta caagccaggg gttacggaca
     8041 acgtggggcg gacagcccgg gaagcggtag agatccttct gcagcggaaa ttgaaccccg
     8101 gcgatggcgt ctacacctcc gtcctatatt ttctgaaggg aaccttggcc cgtaaccagg
     8161 ccgaatccat cgccacgggt ctcctggcca acactctcat ccagaggttt gaaatcaagg
     8221 accgggactc atgggacccg gccgtgggaa tgggggttac tgtacccaag gtttccgcta
     8281 aaaatcaagt ccgggtggaa gagctgaacc tgcaggtcag cgatgaagaa cttcttcgga
     8341 tcagctcgag gcgggtgctg gccttgagcc tgaaggagat gaaggctctc cagcaatatg
     8401 ccaaggaccc gtcacgcctg gcggagagga aaaaagtcgg cctgggggaa cacctcaccg
     8461 attgtgaagc ggaggctctg gcccagacct ggtccgagca ttgcaagcat aagatcttca
     8521 acgggctgat ccactacatc gatgagaagg ggaaagcacg gcgcatcgat agcctctttg
     8581 acagttatat caagcgatcc accgatgaaa tccgcaaggc cttgggggag aaggactggt
     8641 gcgtctcggt cttcaaagac aatgcgggag tcatgagatt caaccgggac tggaacctgg
     8701 tcttcaaagt ggagacccat aacagcccat cggccctgga cccttacggg ggcgccctta
     8761 ccgggatcgt gggggtcaac cgggacccct tcggcaccgg taaaggggcc aagttgatct
     8821 tcaacgtgga caccttctgt tttgcccccc ccgacttttc caaacccctt cctccccgcc
     8881 ttcttcatcc caaacggatt tatgaaggag tgcgggaagg agtcgaacat gggggcaata
     8941 agagcggaat ccccacggtc aacggctcca tcgtatttga tgaccgctac gcgggtaagc
     9001 ccctggtcta ctgcggaacc ggtggaatca tgccccggct gatccgggga gagccctccc
     9061 accagaagaa ggccgagcct ggagacttta tcgtcatgac cggcggaagg atcggcaaag
     9121 acggcatcca cggggctact ttttcctcgg aagaactgca tgaggaatcc cccaccagcg
     9181 ccgtgcaaat cggtgatccc attacccaga agcggatgac cgatttcctt ctccgggcgc
     9241 gcgacgcagg gctctaccat gccatcacgg ataatggggc aggaggattg tcctcttctg
     9301 taggggagat ggcccgcgac tgcaacggtt gcgagatgag tttggagcga gccccgctga
     9361 agtacgccgg cctcgacccc tgggaaactc tcctctccga agcccaggag cgcatgaccc
     9421 ttgcagttcc cccggacaag atcgaccgct tcatggaaat ggccgggctg atggaagtgg
     9481 aggccaccat ccttgggaaa ttcaccgata cagggaaatt tcatgtccta tatgatggaa
     9541 agactgtagc ctacctggac atggaatttc tccataacgg ctatcccaaa atggagctgc
     9601 gggcgaagtg ggaacaaaaa gaacatcccg agcctcagtc cccggacccc aaagacctga
     9661 cctcgacctt gaaacagatt ctctcgcgct acaatgtgtg cagcaaggag tcggtggtcc
     9721 gtcagtatga tcacgaggtc caggcaggaa gtgctgtgaa acccatggtg ggagccttta
     9781 acgacgggcc cggagacgcc tcggttttgc ggcccatcct ggactcctgg gaaggggtcg
     9841 tggtctcctc cggaatctgt ccgcgctaca gcgatatcga cacttaccac atgatggcct
     9901 gcgccatcga tgagggcatc cgtaacaacc tcgctgtggg cgggaatttg aagcagatgg
     9961 ccgggcttga caacttctgc tggtgcgacc ccatccagtc ggagaagact ccagacgggg
    10021 aatacaaact ggctcaactg gtccgggcga accaggctct ttacgaatac accaaggctt
    10081 tcggagtccc ctgtatatcg gggaaggact cgatgaaaaa cgattacctc atcgggtcca
    10141 tgaagatctc cattccccct acggtccttt tttcagtggt gagcgtggtt cccgacgtcc
    10201 gcaagtgcgt aaccatggat gccaagaggg cggaggacat ggtctatgtc ctgggggaga
    10261 cccgggcgga aatgggagcc tccgagtatt atgctctcca tggcttcatc ggcaaccagg
    10321 ttccccgggt ggatgcccaa aaggccagga atctctttca atcgctgagt gcagccatgg
    10381 agaagggatt ggtggcttcc tgtcatgact gctcggatgg aggattgggc gtggcccttg
    10441 ccgaggtggc ttttgccgga ggtctgggaa tagaagtcga tctgcggcgg gttccggcct
    10501 caggcatgga ccggaacgac tttgtcctct tctccgaatc ccagagccgg ttcgtggtga
    10561 ccgtccatcc ggaaaaccag aaagcgtttg aaaactccct ccaaggaaat gtctttgccc
    10621 gggtggggcg ggtgaaggcc ggtcaggacc ttgcgattac cgggctgcag ggacaggtgg
    10681 tggtccgcgc ggacatcgat gaattgaagg aggcctggaa gaaacccctg atgtggtaaa
    10741 ggacaaagag catcgggctt agtgaaaaga ttttttgggc gctatgcggt ccgcgctagg
    10801 ctccatgcct tctttcttga ggtgattatg aaaaaggtga aagctctgat tctggcgggg
    10861 aacgggatca actgtgaaat ggagatggct cacggctgca ggctcggcgg ggcagatgtg
    10921 gcggacattg tctacattta cgatctcctc tctggagaag ttcggctcga cgattaccac
    10981 ttcctgaacc tgccgggggg attcctggat ggagacgacc tggggtcggc caaagccatg
    11041 gcccatcggc taaaatacgg ccgtattgcc ggaagcgggg agccgctgga aaaggggatc
    11101 ctgcgcttca tccgcgaagg caagttgatc ctgggggtct gcaacggctt tcagctcatg
    11161 gtgaaatccg gtttgattcc cgggctgggc aaaatctatg gacaccagac cgtaaccctg
    11221 accttcaacg attcgggccg gtttgaggat cgttgggtgt atttgaaggc aaatccccaa
    11281 agtccctcta tctttaccaa agggctgaag ggactctatt tgccagttcg ccacggagag
    11341 ggaaagttta ttcctcggga tgaagaagtc ctggctgaac tggcccggaa aaacctaatc
    11401 gccctgcagt attcggaccc cgattatcaa aaccccacca tggaataccc tttgaatccc
    11461 aacggggcag tctcggccat cgccggggtc tgcgacgaaa ccggccgcct cttcggcctt
    11521 atgccccacc ccgaggccta cctctatttc gtgaatcatc ctcggtggac ccgggaaaag
    11581 gtcccggaag aagggatggg tctggccctt ttcaggaatg cggtggatta tatccggaag
    11641 aacctgtagg tacgccctgg gcagggggaa tctggatgga tgtttgtagg ggcgacccgc
    11701 cgggtcgccc ctacgatttt gggcggtcag agctatttct tcaagagaag gtcgtgctcc
    11761 gccaacaact tcttgatcgt ttccgtctcc ccccggatat acttgatgga ctgctcgccg
    11821 ttcaggatta ggggaacgaa tcccgccgag atgatcttgg actgatactc ctccttattc
    11881 acggtgtcca aaaaagcctt ctccaatcgg gtggtgatgt ccggcggcgt attcttgggc
    11941 accgccactc cccggtcaat ccgcggatag aatttatggc ccagttcctg gaaagtggga
    12001 acattgggga gaacgttcat ccgcttctct gtcccgatcg ccaggatctt catctggccg
    12061 gcctgcgcca ccaagtcgga agagtttccc cagattgcct ccacgtggcc gccgagaaga
    12121 gccgtgacct ggggggccgc cccggtaaag gttaccaggg tgagcttgat attgttcatc
    12181 ttcatgaaca atagggaggc catgtggtgg ccggagtatt tcccggacat ccctatggtg
    12241 atcttgttcg gatttgccct ggcgaattcg atgaattcct tcacgccatt gatcttcgaa
    12301 tccttcttga ctgccagacc gatgggcgtg gattcgaaga tgcagagggg tttcaaatcc
    12361 tcggtttgga aggccacatc cgactgtgaa agaggctgca ggatgatatg ggggatgttg
    12421 atgccgcaca ccgaatatcc atcgggcttg gcctgggaaa acttcgtcca cgccagtcca
    12481 ccgcccgccc cgggcacgta gttgatcacg atgggaaccc caaggttctt ctcaaggtga
    12541 ggttgctgca tccgagcttc tacgtcggat tggccgcccg gattgaaagg aataatatag
    12601 gtaatcggcc ctttcggaaa gtccgccgcc gggaaagcca cggccgggag aagaaccagg
    12661 gccagggata tcatactaac gaagattttt ttcatctttc cctccttttt cccttcaaaa
    12721 aaccatacca aaccaattcg gcacagccgg gacaaaaaat ttcttctagg gccccacttc
    12781 aacttggccc cccgtttctt ttgttaggcc gacggggctg ggttcgagca tttacctgat
    12841 gaggcccccc gcgctctcta cggttaaaaa tttttttact gctttttcag gtcaaactcc
    12901 tgcgccagtt tcatgtgctc cttcacttcc atatccaggt atttgtgcga ttcttcgatc
    12961 cccatcacct gggggacgaa accggcctgc tccatcttgg aaataaattc tggctttgaa
    13021 atggtctcta ggaaggcttt ctccaggcgc ttctggatat ccttgggaat cgccttgggc
    13081 cccatggcgc ctcgggcaat acgtgggtac attttatagc ccagttcttc aaaggtggga
    13141 atctcgggca gctggaccat ccgtttgggg gaaccgatgg ccaggacctt gatctggtct
    13201 ttgctggtcg tcaggacgct tgaattggtg aaagccgcat cgatgtgccc gcccagtagc
    13261 gccgtttgaa cctgggaggt gccagtgaag gtgacgtagt tcaccttcac tccggctagc
    13321 ttcataaact gaagagtggc aaaatggtgg ccggtgaatt ttcccacccc gccgcaggag
    13381 accttccccg gattcgcttt gcaataatcg atgaactcct tgaacgtatt ggccttgaaa
    13441 tcctttttca ctcctaaacc gatgggcgta tactccacga tggagagaat cacgaaatca
    13501 tcggtcttga aagtcacatc cttcatgaaa ataggctgga ggatgatgtg gggaatgctg
    13561 aatcctccga tggtgtaccc atccggcttg gccgcggcca gcttcgacca gcagagcccg
    13621 ccccccgcgc ccgggagata attgatcacg aatggaactc ccaggttctt ttctaggtat
    13681 ggctgcatag cccgggccgc tatgtcaacc tctcccccgg ggttaaaggg gttcaggtaa
    13741 ttgatggggc ctttgggaaa gtcctgagca ttggccacac tcgatgaaat agccagcaag
    13801 aacccgaaaa tcaaacacgc tgcctgccat ttcttcatct cttctctcca tcctttcttt
    13861 ctttttctct ctcctacacc tcttctcctt caaccttttc ccgcagcttc tggcgccgtt
    13921 ttcccaacca ggggatgaaa ggtgaaatca agagaaccaa ggaaataacg atgaacacag
    13981 cagaaatggg tttttggatg aaaatccaag ggtccccttg ggaaatcgta agggccagcc
    14041 ggaggttgtt ttccatcatg ggtccgatga cgatggctag gaccagcggg gccatttcaa
    14101 attgaaactt ccgggccagg tacccgaaga ccccaaaccc gatcatcaaa gacacttcta
    14161 cgttactata gttcaggctg aataccccga ccagacagaa gagcaggatc agggggaaga
    14221 ggatggggta agggactttc aaaaccttga cccagaggcc gatgagggga aggttgagga
    14281 tcaggagcat gacgtttccc aggtacatgc tcatgatagt cccccaaaag aggtccggat
    14341 acttggaaat caacatgggg ccgggctgga tgccgtggat catgaaggcc cccagcagga
    14401 tggcgataac cgaattggcc gggatcccca gggtaaacag gggaatgaag gcgcccccgg
    14461 tggccgagtt attcgccgac tccgggccgg ctactccttc gatcgctcct gtgccaaact
    14521 tctctggatg cctggaaagc ttcttttcga ccgcataaga cgtgaaagcg gaaatcaccg
    14581 gagctggccc gggcaggatt cctatgaaga agcccagaaa agacccgcgg acaatcggcc
    14641 agacagagtc tttccaatcc tggaggttgg ggaggaggtc tttcaccttg gcggggataa
    14701 tttcccgctt tatctcctct tccacgttgg agatcacctc cgccaccccg aaaagaccca
    14761 tgatcgccgg tatcagcccg atcccatcgg ccagttcgag gaccccaaag gtcaaacgct
    14821 gggtcccgga gatggcatcc atgccgatgg ttccgaggag caacccgaag accgctatca
    14881 ttagagcttt caagatcgac ccagaggcga ggtaaataag gacgacaatt cccatgagca
    14941 tgaggctgaa gaattctggg ggaccgaaag ccaaagcaaa tttggccagg ggtggagcga
    15001 ccagcatcag tccgagcaca gaaatggttc cggcaataaa agacccgaag gcggcaatcc
    15061 ccagggccgg gccggcccgg cctttgaggg ccatctgata cccgtccagg caggtaatga
    15121 ccgaggcagc ctctccggga atattgacca ggatggatgt ggtggaaccg ccatacatgg
    15181 ctccgtaata gatcccggcc agcatgataa tggccgttac ggggggcacg tggaacgtat
    15241 tgggaaggag aagggcgatg gccgccgtgg gaccaaggcc ggggaggaca ccgaccagtg
    15301 ttcccatgag cacgcccagg aaacagaata cgaggttcgt tggggtaaac aggacttggg
    15361 cgcccaaaag gatgtgggat agtatatcca tagaccacct agaatccaag gaatcccttg
    15421 gggagctgag tctcgagaaa attcacgaag agtagctggg ctgccaggga actgaggacg
    15481 gcaataacta gcgagcgagt ccaggtttgg ggaaagataa agcggaccag aaagcctaca
    15541 aatagaaagg tcattagggt gaaaccaagg gttgtcagga gaaggttgta ggcaattaag
    15601 gcggcaaatg tggccgttac tctcttccag gagtccgatc gcgagaaaaa ggaatgggtg
    15661 atggccggta gctttcccca gaacgtgcgc atgagaaggg caatggacat cgctgtcagt
    15721 acaatcccgc cccagaaggg cagaaaacca gggccgggtt cattccaggc tccgatttcc
    15781 accgaccggg catagatgga aaagaataaa cctacaatca ggaagaagat tgcggatatg
    15841 cggttataac ggatggcttt atcctttttt ctcctgcacc aaggccttca ggagactttg
    15901 agcccacatg gaatgcactt ccccggccga ttctgggtgg attctaaacc ggcaaaatgc
    15961 ttcttgtcaa ggatgtaatc ggtttcattg aacaagctca ccgggctaat catcgatcct
    16021 tgatcaccgg tatcccaagc cgacggggct actactccct atggacggcc gctgggtatt
    16081 agatgatctc cgttggttaa ggttgctttc aaccggaaat tatcgccttt gggaattgat
    16141 catctgcatt tttacgtaag atgaggtaag attccaggag atgaactcgt aggattcggt
    16201 ggggaaaccg aagaagtcct cagagtaccc ggagttaccc ctttcttcga atctcttcga
    16261 gcacgggaat tatgtatttt cggaattggg cggggttctc gctcatcgga aagtgtccca
    16321 actctttcat gatcatcact ttaacaccgg gaatggtgtt cgcggtgcgc agggtgtcct
    16381 caggctggca ggaaaaatcg tattccccag tgagtaaata aagcgggcaa gctgcggtgt
    16441 taatcccatc cagctttccc cgcaggtcgg catccacccg atagaaatag aggtcccccc
    16501 gaaacacgcc agggccgccc tgcatgtatt gccagagggt ctcatggcgg tgctcgtctg
    16561 ggctttgagg agcgatcaaa ccggatatca gggcggcgca aacttcccct ccatgcacgt
    16621 cgccgcgatg aagccagttg gtgtcgtacc acggctgctg aaagtcggcg gattcgatgc
    16681 cgatcaaggc tctaaattca gaaccatgag catgggcgag ttggaggatg atccggcctc
    16741 caatggaaca tcccagggca acagggtgtt ctaggcccag cgcggcgcag aaggctcgaa
    16801 tggtcgccac gtatagctcc gtagtgagcc ggtactcacg cttttcccag cccgctggag
    16861 gataggactt gccatgccag ggtaggtcga aggcgatcac gcggaattta ttggtcaccg
    16921 tttcgtcgca cataaggtgc cggaattgcc gcgagtcggc tccggcggta tggaggcaca
    16981 ccaggggaat cccctgtccc gtttcttcga aatagacccg gcagtcgtct cccatgatcc
    17041 ggactttcat ataccgcccg atgataggct ccatgtcctt cttgttcacc gggaacctcc
    17101 tttccgcggc aatgcaacga cgtccttgaa atactggagg ttcgccatca aggggtgcag
    17161 attcccctcc agggccatct ccccccgctt ggtgagggcg aaaatatcat gccaccccgg
    17221 cggcggtggg tcctgccaga gagccgccca ggcctgcgcc gagccccgca ccgcaaacac
    17281 ccaggaacac aatagcggca atcccttgcg acattcgcga atgcttccct cctcaatccg
    17341 gagcagaaag ggaacggtcc cgatttgaac caggcaatcc accgtcagat tccgaccccg
    17401 ccaaaccagg cgccggtctg agttcaccgc ttccggaatg acccgctcaa atcgggcact
    17461 gagcgcttgc tccagtcctg cgggggaatt ctttgaatcc tccggcatgg atctcctccc
    17521 tcttgtctat cgttgcgtaa ggctaaacgt tgcataaaat tctcatggag taagtttgtc
    17581 atctccccga aagcgggaat ccagaaagaa atcctggatt ccgggtcaag cccggaatga
    17641 cggaagctaa atgggcggcc tatttttcac ttcttacctc aactcccgcc agcttttcca
    17701 aaggccggat gaccgcagga tggtatttct tgctctctcc tccggctccg gccgaaatat
    17761 attgctgggc ggacagagcc gcaaaatgga gaggaatccg cagtttcgat cccgtcgata
    17821 gggccaggtc caaatctttc aatatgtagt cgatgggaaa gaccccttct tcgaactttc
    17881 ccttcatgac attattcttg taatggttct gcaggacaaa actatttcct gatccgctgg
    17941 agagggcttc aaaaaggatg tccggtttca caccggcctt cacccccaag accatggcct
    18001 cggccagggc gcaggtcgtt accgccacca tgagattgtt gacgatcttg accacctcgc
    18061 cgctgccgat gtcgcctacg tgatagatgt ccgttcccat ggccgccaga atcggccggt
    18121 ggcgttcaaa gacctccttt ttcccgcccg cgtaaatgac cagcgttccg gcaacggccg
    18181 ctggcacccc ccgggccacg ggggcatcca gcatctccac tcccttttct ccgagaatct
    18241 tagcgatgtc cctcgtggtg agggggtcga tcgtgctcat gtcgatgagg acggactccc
    18301 tttggattcc ttcgaccacc ccaccctttc caaggacggc ctccctcaca tgggcggaag
    18361 cgggcaatat ggtgattacc actgggcatt tctcagccgc ttctctgcag gacgcggccg
    18421 tttgggcccc cgcggccttt agcggcccca ttctttccgc aacgatgtca aataccataa
    18481 gcgaaaaacc cttctttacc aaatttttag ccatgggtcc ccccatggcc cccaatccga
    18541 tgaatccgat attctccatt ttctttctcc tgcaaaagtt gaaaaaacct tctttacggc
    18601 ccaaccctgt tttttaaaga ccccatgaat ggtcgaaggc tataggatca aatgaatcgc
    18661 cggctgatca gcctcccagg taagcctttc ggacataatc attatgcaaa aggtcctcgg
    18721 ccttcccctc cagggccacc tcgcccgact ccagcacata ggcgtactcg gcgaggcgca
    18781 acgccatgcg ggcgttctgc tcgacgagga tgatcgatac tccggctccg ttgatttgct
    18841 tgatgattgc ggctatggct ttgaccacca gcggagccaa tcccaaagaa ggctcatcca
    18901 gcagcaggta ttggggcttg gccatgagag cccggccgat ggccagcatc tgttgctccc
    18961 ctccgctcag cgttccggag acctgtttct cgcgcttttt cagggcgggg aagtagccat
    19021 agacccgttc ggtatcctcc cggacctctt gccggttgcg gcggagatag gcccccaggc
    19081 gaaggttctc ctgagcggtc aattccgaaa aaaccctcct cccctccgga cagagggcga
    19141 tgccctgacg gatcacttcg gaggtgtcca gccggtcgat ccgcttcccc cggagataga
    19201 tctcccccga agtgatcggc tttagaacgg tgatggcctt gagggtagtg gtcttgccgg
    19261 cggcattcgg cccgaccagg gccacgatcg agccctcctt cgccgagagg tgaatccctt
    19321 tgagggcctg ggtttggccg taatccactc tgaccttgac cagcttaagc aatctccgta
    19381 tcctccccca ggtaggcttt gatcacctct tcgttccgct gaatttccgg cggggtcccc
    19441 atggcgatca tctgcccgta attgagaacc aggatccggt ggcacaggcc catgatgact
    19501 ttcatatcgt gctccaccag gatcaccgtg accccatttt cctgaatctt ctggatcgca
    19561 ctcatcagat gtacccgctc ctcgtgattc aagccagctg cgggctcatc cagcataagg
    19621 agctggggat cccccgccag ggcaatcgcc agccccagaa gcttctgata gccatagggc
    19681 agggccttgg ccgcctgacc ccgcatcttt tccaggccca ggaatcggag gagggtttcg
    19741 gccttctccg tctggttttt tttccgttcc cggaccgccc gattgattag aaattcgggc
    19801 cacaagccca ggaagtgata tcccaattgg gccctcatca tgttttccca caccgtggcc
    19861 tccccgtagg aaacggtgga ctgaaaagtc cggaccaggc ccatcttggc gatcctatac
    19921 ggggggtagc cgactatgct ttctcccttg taccggactg tccctgaatg aggggcgtac
    19981 atgttgctga tcaagtttaa caaagtggtt ttgcccgcac cattcgggcc gatggtaccc
    20041 aggatctccc ccggctggac ggagaaggtt gcctgatcga ccgccatcag cccgccaaat
    20101 tgcttggata gcttttccac ttggaggata ggggtcatgg agtttcttcc ttgccaggct
    20161 tccttgattt catccggtcc aaggccttat tcagcaaccc caacatgccc gtgggcatga
    20221 accagagggt gaggatcaga atcagcccgt agagcaccct ttggtattcc acgaatcccc
    20281 tgaggaactc gggcagggga accagaaaga tcgagcccag gatcgggccg gcgattccgc
    20341 ccatgccccc cacgacgttg atcacgatga gattcacgct ctcccagaag gtaaacgatt
    20401 ccggggaaat gaaccggtag tagtgagcaa acagacttcc cgcgagtccg gcaaaaaaac
    20461 ctccgccggc aaaggcgaac atcttgtgtt gaaaggtgtc gattcccgtg aactccgcca
    20521 gatgttccgt ctcccgggtg cagtccatgg ctttcccgat ggaggattga taaatccggc
    20581 gaatgataaa cagagccatc agcataaccc ccagcaccag atagtaataa gaaaccttag
    20641 acagaagggt gaaggcggtg cccgggaaga gcgcaatcct ggggggaggg atgttaataa
    20701 ttcccgtggg tcccccgaag ggttcgacga gattcacgaa gaagagcctg accacttcgc
    20761 caaaggcgaa ggtcaccagc acaaaataaa ccccctggag cttcaggagc aggcgcccga
    20821 ccaggagagc gatgcaggct cccatcagac ccgacagagg aagggccagg agatagggaa
    20881 ggcccagccg catgaccgtg agggcggagg tgtatcctcc gatccccatg aaggcggcgt
    20941 gggcgatcga taatttcccc gtcttgatga ccatattcag gctgacggcc agaataatat
    21001 tgagatagat gctgatgatg acgttgaggt agaactcatc agcccagaga accgggatga
    21061 agaaaagaaa tacggagagg gccaggagag cgtaggattt gcttttagga atcactttcc
    21121 cctcttcctg catcgggtcc ggtccggtcg ttcccaagat gggccgcctt atcagcggct
    21181 cacctgggaa cggccgaata ggccggaagg ccggaacagc aggatcccga tcaccaggac
    21241 gaatcccagc aggtcggcca acgccttgct cgcataggta ctgacaaaac tctctgccac
    21301 ccccagaatc aacccgccca ggaaagcgcc gggaatggac ccgatccctc cgaggattac
    21361 gacgatgaag gccttgacct ggggcatgat tcccatccac ggattcaact ggaaaagggg
    21421 accgatgacc gcaccggcgg cggccgccag tccagtgccg acggcaaaag ccagaggata
    21481 caggcgattg atcctcatcc cctgaaccct ggcgatctcc gtatcctggg caatcgcctg
    21541 gatggccagg cccagtctgg tctttttcag aaacaggtag aacccagcca tgagcaggaa
    21601 ggagataaac accaccatca ggcgttccat cgggtagaag agggtcccga tcttcaggac
    21661 cccggtaaag acgggaggga tggacagatc gtaggggccc caggccacca acccgagatt
    21721 ctgcaggaga atggaaagac ccagggcgat gatcattcct tccagctctt tgtcccgaaa
    21781 ccggcggaag atcacttctt ccgtcaaata ccccaaaaga ccgatgaaca ggaccgcgag
    21841 aatcgccgcg agaagataat tgacctcgaa tttcaagata gccgtgagga caaagaaggc
    21901 ccccagcatg taaaattcac catgggcgaa gttgacgatg cgcaagacgc cgaagatcag
    21961 ggtgaacccc agggccatga ggatataatt ggtccctagg gtcaccccgt tgatgatcac
    22021 ttggggaagc aacgtctcgc ccaacgacta tcctcctcgc cgcattccgc tttttggaaa
    22081 gatgggtttt acgatccgcc ccgccggaag gctacggcgt cgctttcccg agaacgactt
    22141 ctttgccgtc cttcacgacc atgacgtaga aggggaactt gacttggtga tcgatcccgt
    22201 aatccgcctt cccggtccag ctcatggctc cggtgtacac gccgttgaag gaaaggcttt
    22261 ccagggtcgc tctcaccttt tcggtatccg tgagggtccc ggccttcttg attgcctcga
    22321 aaagccataa ggttccgtca tagaactggg gagtaaagcc attcatggga ggagggaatt
    22381 tggcattata cttcttattg atctcctgga tcttgggatc atcggggttg atcggactgt
    22441 aatagataaa tccttccatg aaatccttgc cagccacttt aagcatttcg ggggtgcccg
    22501 gccctcccac cttgatgatc atgcctttgt agcccatctg gcgggcctgc tttacgatca
    22561 ggccgcaatc ccctggattg gacccgtcga ggttcaacac atcgggtttc a
//
LOCUS       DASPRR010000045         9645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2271833, whole genome shotgun sequence.
ACCESSION   DASPRR010000045 DASPRR010000000
VERSION     DASPRR010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271833"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..827)
                     /locus_tag="VEL68_01735"
     CDS             complement(60..827)
                     /locus_tag="VEL68_01735"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ34719.1"
                     /translation="MAEYNTMELMIVVAARNLENGKTVGVGTGAPCAAAMLAQKTHAP
                     DLVIMFEAGGISPQLPTMPISVGDSRTFYRAVMASGMCEIMETCQRGMVDYTFLGGAQ
                     IDMYGNLNSTVIGDWKKPKVRFPGSGGANDFASFCWRVMVITPQDARRFVEKVSFITT
                     PGYLNGPGAREKAGLPANTGPHKVITNMAVMGYDEKTKRMRVESIHQGNTFEEVQKNC
                     GFPLLKAEKTITTPLPTTDELRILRTEVDPKRYIIGR"
     gene            complement(833..1834)
                     /locus_tag="VEL68_01740"
     CDS             complement(833..1834)
                     /locus_tag="VEL68_01740"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ34720.1"
                     /translation="MFEVVAQGEGPLFMDPDADDAREFFQKKDRTLKSKVMSVKEAVE
                     KFVRDGDYLGIGGFGANRIPTAICHEIVRQKRKNLIFAGHTSTHDFQILAAGEVFNRC
                     DIAYIIGLEARGLSPNARRYMESGKVESCEWTNYGMALRFKAAAMGVSFLPGRNMMGT
                     DTFKYSAAKVVKCPFTGKLFCLYPALWPDVSAIHVHESDIYGNCRLKGISIADLELAR
                     ASKRLIITCERLVHNDVIRNDPTHTAIPYYLVDGVCEVPYGSFPGNMPYEYFSDEEHL
                     RLWMKSEEDLEKFKKFLNQYIYWTKDFNEYLDLCGGLQRINDLRAEEHLIQIKNQKE"
     gene            complement(2241..3593)
                     /locus_tag="VEL68_01745"
     CDS             complement(2241..3593)
                     /locus_tag="VEL68_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014288390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXZ34721.1"
                     /translation="MLQKLASASTRWSTRWVPDAWVIAVILTIVAYFLGIIFTKSNSY
                     QLIQYWGRGFWVLLSFAMQMCLIIMTGYILATTPVFSRILNGLAGVPKSPKGCIALMA
                     LVSLVLAWINWGLSIVGSAVFVRFLAKKQKGVDYRLLVATAYLGLGTMWHSGLSASAP
                     LLVATPKHFLEKELGIIPITETIFHPFNLLLALVVLIALTIMGPLMHPKKGEQVEADP
                     QLIKESGFERPPKPKTADMNPALRLENSPVINIIVGICGLIWLVWHFGQRGFAGINLD
                     VVNFAFLMVGILLHWTPVSFLKSAQEGGSFIWGVVVQFPFYAGIFGIIQFSGLAEVMA
                     NWFTAIATKETYPFIVVWYSGILNYIVPSGGSKWAIEAPYIMQAAKTLGVSNAMTVIA
                     YAWGDMVTDVIQPFWALPLLGVAKLGFRDIMGYCMVYFLFYMVIISIGFLLLPVFFAV
                     "
     gene            complement(3799..5757)
                     /locus_tag="VEL68_01750"
     CDS             complement(3799..5757)
                     /locus_tag="VEL68_01750"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HXZ34722.1"
                     /translation="MKTPLWTPSEERIRNANLTRFIEYVNGKHGKKFKTYPDLYQWSI
                     EDIPSFWARLWEFVDIKASRGYTHVVDDLKKMPGANWFKGARLNFAENLLRYRDDRLA
                     FIFKGETQKYARMTYRELYTTVARLAKALRETGVKPGDRVVGYMPNLMETAIAMLAAT
                     SVGATWSSCATDIGSQAALDRLGQIEPKVLFTVNGYYYKGKVFSSLGNAAEVAKGIPS
                     LRKVVVASYPDEKPDLRQIPNAVRWEDFLSREKDPALIFEQLPSDHPVYIMFSSGTTG
                     KPKCMVQGAAGILLEHLKELVLHTDLKREDRIMYITTCSWMMWNWLVSSLGAGATVIL
                     YDGNPSYPDDGAIWKLIQDEKITIFGTSASYINYIKSQGLKPGRDYDLSSLRAISQTG
                     SPLSAEGFEYAYQEIKKDLHFGSISGGTDINGSFAGGSIISPVYAGELQGPTLAMKIK
                     AYDEKGQPIYDRQGELVCEAPSPSMPLHFWNDPTGEKYKSAYFEVYPNVWRHGDYIVF
                     HSDTKGLTFYGRSDSVLKPSGVRIGTAEIYNVLDKFGEVADSLVIGQNYKGDQRILLF
                     VQLRPGSELTDGLKDKIRKTLREQASPRHIPAVIMAVPDIPYTLNMKKVESAVTNIVN
                     GRPVLNRDALKNPECLDYYEKVLPDLQK"
     gene            6279..7274
                     /locus_tag="VEL68_01755"
     CDS             6279..7274
                     /locus_tag="VEL68_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ34723.1"
                     /translation="MKNILIVEDEFSIRESLQTWLMDLGYQVEVAREGEEALKRIEEK
                     DFDVLILDLRLPGKDGLQVLKEARTKKPKLHGIIITAYPTVEKAVEAMKGGAVDFLPK
                     PIDLSYLEKLVQEKTEAAKAPMKPILVVDDEASMRESLRDWLKDLGYEVEIAADGETA
                     LKLISERDYGLLVLDLKLPGKNGIEVLKEARQRRPGLRGIIITAFPSVDTAVEAMREG
                     AVDYLPKPFTLSELEKLVEENIETAHVAILPQPVSKKAIAAILKILSRAGEDSQFLAR
                     MAESPKEALREYPDLTEEEKEAIMDGDIKKIESWVGKLDERKATWLWCRLSQEKW"
     gene            7268..9241
                     /locus_tag="VEL68_01760"
     CDS             7268..9241
                     /locus_tag="VEL68_01760"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXZ34724.1"
                     /translation="MVNRLSLRTKLIGSFLFVVVAGGILSSLIGTRLVADAIVSQARN
                     KVKHDLNTAWLIYNQSLDRIRDVVQLTAAGRTLPELMESRRWKSLEEFLIKRRKDFGL
                     DTLTLADARGKVLLRTHHPFRTGDDQGFDPLVQRALKGEPAASTAIVPREELEKERND
                     LAERAFTLFVPTPKAKEVPVDRQTSGMMLKAACPVWDGSGRLMGVLYGGVLLNRDFQI
                     VDKVRDLLYGEGRYKGKEMGTATIFQGDLRISTNVLDEKGQRAIGTRVSEEVYQAVLE
                     KGELWLDRAFVVHDWYLTGYQPIKDITGKIVGILYVGMLEAPYVDLRNRVVYSFFGIG
                     VLGVVIVVLLYFFITSGITRPLREMAAATGKIAEGDLSIELSSKSKDEIGQLAQSFNY
                     MLVRLKQARKELEDYGRVLEVKVEERTQELTKMQDHLIQSEKMASMGQLAASIAHEVN
                     NPLSGVLIYNQLIAKKITRGDFDREVILDYLSKMEHELSRSTKLVRSLLDFGVQTEPK
                     IKEVNVNEVLNRALELGIHAGYKENTRVEKDLRPLPTFMEDPDQLEQVFVNLIMNALQ
                     AMPTGGKLTLRTFMENREVKIAVQDTGCGIPPENLSKIFTPFFSTKKEVKGVGLGLSV
                     AYGIIQGLKGRIEVESKVGEGTTFTVCLPITQA"
     gene            complement(9501..>9645)
                     /locus_tag="VEL68_01765"
     CDS             complement(9501..>9645)
                     /locus_tag="VEL68_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXZ34725.1"
                     /translation="SEALPPGVVFMTFHFKEAAVNLLTLDALDPVAKIPGLKVCAVNI
                     KKV"
BASE COUNT         2236 a         2656 c         2732 g         2021 t
ORIGIN      
        1 aaaccccgct tccccttccg agaaacgggg tttttatcct gtgccgttga acctaaaacc
       61 taccggccga taatgtagcg cttggggtca acctccgtac ggaggattct cagctcatca
      121 gtggtgggca gaggagtggt aatggtcttc tcagccttga gcagggggaa gccgcagttt
      181 ttctgcacct cctcaaaagt attcccctga tggatcgatt ccacccgcat ccgcttggtc
      241 ttttcatcgt atcccatcac cgccatattg gtgatgacct tgtggggacc ggtgttggcc
      301 ggcagccccg ccttctcccg agctccggga ccattcaggt atcccggagt ggtgatgaag
      361 ctcacttttt ccacgaacct ccgggcgtcc tgcggggtaa tcaccatgac cctccagcag
      421 aaggaggcga aatcgttggc tccgcccgac ccggggaaac ggactttggg ctttttccag
      481 tccccgatca cggtggagtt caggttgccg tacatgtcga tctgggcccc gcccaggaag
      541 gtgtaatcga ccatccccct ctggcaggtt tccatgatct cgcacatgcc gctggccatg
      601 accgcccggt agaaggtccg cgaatccccg acggagatgg gcatggtagg aagctggggc
      661 gagattcctc cggcctcgaa catgatcacc aggtccgggg catgggtctt ctgcgccagc
      721 atggccgccg cgcagggagc cccggtgccg acccccacgg tcttgccgtt ttccagatta
      781 cgggcagcca ctacgatcat caattccata gtgttatatt cagccatctc ggttactcct
      841 tttggttctt gatctggatg agatgttcct cggctcttag gtcattgatc cgctggaggc
      901 ccccgcagag gtccagatac tcattgaaat ccttggtcca gtatatatat tggttcagga
      961 acttcttgaa tttttccaaa tcctcctcgg acttcatcca gagccgcaaa tgctcttcgt
     1021 cggagaaata ttcgtagggc atattcccgg gaaagcttcc gtaaggcact tcgcacacgc
     1081 cgtcgacaag gtaatagggg atggccgtat gggtggggtc attccggatc acatcgttgt
     1141 ggaccagccg ctcgcaggtg atgatcaaac gcttggaagc ccgggccagc tccaggtctg
     1201 caatggagat gcctttcagc cggcagtttc catagatatc ggattcatgg acatgaatgg
     1261 cggagacatc gggccaaagc gccgggtaga ggcaaaacag ctttccggta aagggacact
     1321 tgaccacctt ggcggcgctg tatttgaagg tgtccgttcc catcatattc cgcccgggga
     1381 ggaaggacac ccccatggcc gctgctttga aacgaagggc catgccataa ttggtccatt
     1441 cgcagctctc caccttcccg ctctccatgt aacgtcgcgc attcggggaa agtcccctcg
     1501 cctccagccc gatgatgtag gcgatgtcgc agcggttgaa gacttccccg gctgccagaa
     1561 tctgaaaatc gtgggtcgag gtgtggccgg cgaagatcag attctttctc ttctgccgga
     1621 cgatctcgtg gcagatcgcc gtggggatcc ggttggcgcc gaacccgccg atgcccaggt
     1681 agtccccgtc gcggacaaat ttctccacag cttctttcac cgacataact ttgctcttaa
     1741 gggtccggtc cttcttctgg aagaattccc gggcatcatc ggcatccggg tccatgaaga
     1801 gagggccctc tccctgagca accacttcga acatatctcg ttatcctcct ctttcggtaa
     1861 ataataaccc aaaatcccct accggaatga caggaatcag aggcgaggaa aggggaaaaa
     1921 gcagggcaga ttaatctttt aaaaatctag caatagaagt gccaaataat gggaggaaac
     1981 atccctttct aataatataa ctatttgaaa ttaaacttct tttttccaaa ccctcgggta
     2041 gggtcgggga agaatgaatt catattcatt ctatagacaa agtgtcattt ttgtcaattc
     2101 tttccggtgt aaaatgacaa ttattcaaaa aatgaagaaa aatccgtttt gagtgtcaat
     2161 ctgactttga gaggggaaga aggaaggcgg gaaggcgccc ttccccctca ttagggggaa
     2221 gggtacgatt aggtttaggt tcaaaccgca aagaagaccg gtagcaaaag gaatccgata
     2281 gagatgatca ccatgtagaa caggaaatac accatgcagt agcccatgat gtcccggaag
     2341 cccagcttgg ccacgcccag aagggggagg gcccagaatg gctggatgac atccgtaacc
     2401 atgtctcccc aggcataggc gataaccgtc atggcattgc tgactcccag agtttttgcc
     2461 gcctgcatga tgtagggagc ctcaatcgcc cacttagacc ctccggatgg aacgatgtag
     2521 ttcaggatcc ccgaatacca gaccacgatg aagggatagg tctccttagt ggcgatggcg
     2581 gtaaaccagt tggccatgac ttccgccagt ccggaaaact ggatgatccc gaaaatcccg
     2641 gcgtagaagg ggaactgaac caccaccccc cagataaagg atcccccttc ctgagcagac
     2701 ttcaagaacg agacgggagt ccagtgcaga aggatgccga ccatcaggaa ggcaaaattc
     2761 accacatcca ggttgatccc ggcgaatcct ctctgcccaa aatgccaaac cagccagatc
     2821 aggccgcaaa ttcccacgat tatattaatg accggagaat tttccagccg caacgccggg
     2881 ttcatgtctg ccgttttggg cttgggcggc ctctcgaacc cggattcttt gatgagttga
     2941 gggtcggcct cgacctgctc gcccttcttt ggatgcatca agggccccat aatcgtcagg
     3001 gcgattaaaa ccaccagggc cagaagcaga ttgaaggggt ggaagatggt ctccgtaatg
     3061 gggatgattc cgagttcctt ttccaggaag tgcttggggg tagccaccag gaggggagcg
     3121 gaggcggaga ggccggaatg ccacatcgtt ccgaggccca ggtatgcggt agccaccagg
     3181 agccggtaat cgaccccttt ctgctttttg gccaggaacc gaacaaagac cgccgacccc
     3241 acaatgctga gcccccagtt gatccaggcc agaaccaggg ataccagggc catgagggca
     3301 atacatccct tggggctttt gggcacgccg gccaagccat taagaattcg gctgaatacg
     3361 ggcgtagtgg ccaagatata gccggtcatg atgatgaggc acatctgcat agcgaaagag
     3421 agtaagaccc agaatcctct tccccagtac tggatcaact ggtagctgtt ggacttggta
     3481 aagatgattc ccaagaagta ggcaacgatg gtgaggatga cggcgatcac ccaggcatcg
     3541 gggacccaac gcgtgctcca acgggtactg gctgaggcaa gtttttgtag cataggattc
     3601 tcctttcaat gaatgaaata gaatcaacta ccctgcaaaa aatctctctt tataccctta
     3661 gacccatccg aactcaatca ggcaagcgtc cggcctatac tacttttcca attagaaaac
     3721 caagatggcc ttttccccaa ccaaataggg gcggttcgtg agccgcacct agcttccgga
     3781 ctcaaaaccc tgttctccct atttttgcag gtccggaagg accttttcat agtaatccaa
     3841 gcactccggg ttcttcaggg cgtcccggtt caggaccggc cggccgttga cgatgttggt
     3901 gaccgcactc tctactttct tcatgttcag agtgtaggga atgtccggca cggccatgat
     3961 gaccgcggga atatgccggg gggaggcctg ctcccggagg gtttttcgga ttttatcctt
     4021 taatccatcg gtgagttcgc tcccgggacg gagttggacg aagaggagaa tccgctggtc
     4081 ccccttgtag ttctggccga tgaccaggct gtcggccacc tccccgaact tatccagcac
     4141 attgtagatt tcggcggtgc caatccggac tccggaaggt ttcagcacac tatccgaccg
     4201 gccgtaaaaa gtgaggccct tcgtatcgct gtgaaagacg atatagtccc cgtgacgcca
     4261 gacgttgggg taaacctcaa agtaggcact cttatatttt tccccagtcg ggtcattcca
     4321 gaaatggaga ggcatggaag gggatggcgc ctcacagacc agctctccct gccgatcata
     4381 aatgggctgg cctttttcat cataggcctt gattttcatc gccagggtcg gcccttggag
     4441 ttcccccgcg taaaccgggc tgataatgga ccccccggca aaagacccgt tgatgtccgt
     4501 gccgccggag atggaaccaa aatggaggtc cttcttgatc tcctgataag cgtattcaaa
     4561 cccttcagcc gaaagagggg aacccgtctg ggagatagcc cggagggagg acaggtcgta
     4621 atccctcccg ggcttcaggc cctggctttt tatgtagttg atgtaactgg cgctggtccc
     4681 gaaaatggtg atcttctcat cctggatcaa cttccatatc gctccgtcat cggggtaact
     4741 ggggttgccg tcatacaaga tcacggttgc tcccgccccc agagagctga ccagccagtt
     4801 ccacatcatc cagctgcagg tggtaatgta cataattcgg tcctcccgct tcaggtcggt
     4861 gtggaggacc agctctttca ggtgttccag caagatgccc gcagccccct ggaccataca
     4921 cttcggcttg cccgtagtcc ccgaggagaa catgatgtac accggatggt cggagggcag
     4981 ttgctcgaag atcaaggcgg ggtctttctc gcgggaaagg aaatcttccc accggaccgc
     5041 gttgggaatc tgtcgcaggt cgggcttctc atcagggtag gaggcgacca ccactttacg
     5101 gagcgaaggg atccctttgg ccacttccgc cgcattgccc agagagctga aaacttttcc
     5161 cttgtagtaa tagccgttca ccgtaaacaa gactttgggc tcgatttgcc ccaggcgatc
     5221 cagggctgcc tgggacccga tatccgtggc gcaggaagac caggtcgccc cgacgctggt
     5281 ggcggccagc atggcgatgg ccgtctccat caggttgggc atgtaaccaa ccacccgatc
     5341 ccccggcttc accccggttt cccgcagcgc cttggccagg cgggctaccg tggtatagag
     5401 ttcccggtag gtcatccgtg cgtatttctg cgtctcccct ttgaagataa acgccagccg
     5461 gtcgtctcga taccgtaaga gattctcggc aaaattgagc cgggcccctt tgaaccagtt
     5521 ggcccctggc attttcttca ggtcatccac gacatgagtg tacccccggg aggccttgat
     5581 atccacaaac tcccacaggc gggcccagaa actgggaatg tcttcgatgg accattgata
     5641 gaggtccggg taggttttga acttcttccc gtgcttcccg ttgacgtact cgatgaagcg
     5701 ggtgaggttg gcgttgcgaa tcctttcttc cgacggcgtc cagagcgggg tcttcatggc
     5761 tacctccttt ggatcagatg ggatgaccct cccttggaga gagggctcat ggatcaggga
     5821 aaaaatcttc tggcggaaaa cccgcaatga acctagcaaa aagaaaagga gaaggcaagg
     5881 gaaaaaagag gccaaaggag cctgaggcac gggccaaata acacttgaca gcgaatcgat
     5941 tttggctaac atgcagaata gagttcgcgc acgttcttgg gccattccat ctattgaacc
     6001 gaaatccccg gaggtgaata tgcgaggcag cgagccccag gtgcccgaag gaggcctctg
     6061 gggtgaaaat catcttcgag cgaagggaaa gctcgatctt cccacccgat tgccagccga
     6121 agcccttttt cccttgattc aagaatcagg tctcgtttcg tcgccggcag aaaaccgggt
     6181 ccagcagccg ccggggaggt cccctgattc ggctggactc cccgagcgga ttcccgtcct
     6241 tcgaataccg aaaacgaaat ttccggagga gagaaaacat gaagaacatt ttgatcgtgg
     6301 aagatgagtt cagcatccgg gagtcgctgc agacctggct catggatttg gggtatcagg
     6361 tggaagtggc aagggagggg gaagaggcct tgaagcgaat cgaggagaag gacttcgatg
     6421 tcctcattct ggacctaagg ctgccgggaa aggacggtct tcaggtctta aaggaagcca
     6481 ggaccaagaa acccaaactc cacggcatta tcattaccgc ctaccccacc gtggagaaag
     6541 cggtggaagc catgaaggga ggagcggtcg attttctgcc caaaccgatc gatctctcct
     6601 acctggaaaa attggtccag gagaagaccg aagccgccaa ggcccctatg aagcccatcc
     6661 tggtggtgga tgatgaagcc agcatgagag aatccctgcg ggactggttg aaggatttgg
     6721 gctacgaggt ggaaatcgcc gcggacgggg agaccgcgct gaagctcatt tccgaaaggg
     6781 actatggact gctcgtcctg gatttaaagc tgccggggaa aaacgggatc gaggtcctga
     6841 aagaggcccg gcagcgccga cccggcctga ggggaatcat catcaccgct ttcccctcag
     6901 tggatacggc ggttgaggcc atgcgggagg gcgccgtcga ttatttgccc aaacccttca
     6961 ctctctccga actggagaag ctggttgagg aaaacatcga aacggcccat gtggccatcc
     7021 tgccccagcc ggtgagcaaa aaagccatcg ccgcgattct gaaaattctg agccgggcgg
     7081 gagaggacag tcagttcctc gcccggatgg cggagtcccc caaagaggcc ctccgggagt
     7141 atccggacct gaccgaggaa gagaaagaag cgatcatgga cggggacatt aagaagatcg
     7201 agagctgggt cggcaagctg gatgaacgga aggccacctg gctttggtgc cgtttgagcc
     7261 aggaaaaatg gtaaaccgcc tgtcccttcg taccaagctg attggcagct tcctcttcgt
     7321 ggtagtcgcc ggggggatcc tttcttccct tatcggcacc cgactggtgg ccgatgccat
     7381 cgtctcccag gcccgcaaca aggtgaaaca cgacctgaac acggcctggc tcatctacaa
     7441 ccagtccctg gacaggatcc gcgacgtggt tcaattgacc gccgcgggcc gtacccttcc
     7501 cgaactcatg gaatccaggc ggtggaaaag cctggaagaa ttcctgatca agcgccgcaa
     7561 ggatttcggg ctggatactc tgacccttgc cgatgcccgg ggaaaagttc ttttgcggac
     7621 ccaccacccc ttccggaccg gagacgacca gggattcgac cccctggtgc aacgggcgtt
     7681 gaagggcgag ccggccgctt ccacagccat cgtgccccgc gaggaattgg agaaggaaag
     7741 gaacgacctg gcggagcggg cctttaccct attcgtcccc actcccaagg ccaaggaggt
     7801 tcccgtcgac cggcagacct cgggaatgat gctcaaggcc gcctgtcctg tctgggacgg
     7861 gagcgggcga ctgatggggg ttctttatgg gggagtcctg ctgaaccgcg attttcagat
     7921 cgtggacaag gtacgggatc tcctctacgg ggagggccgt tacaagggaa aggagatggg
     7981 gaccgcgacc atcttccaag gggatttgcg catctccacc aatgtgctgg atgagaaagg
     8041 acagagggcg atcgggaccc gggtatcgga ggaggtatac caggcagtcc tggaaaaagg
     8101 ggagctgtgg ctcgaccggg cctttgtggt ccacgactgg tatctcaccg gctatcagcc
     8161 gatcaaggac atcaccggaa agattgtggg aattctctac gtgggaatgc tcgaggcccc
     8221 ctatgtcgac cttaggaaca gggtggtata cagcttcttc ggcatcggcg tcctgggggt
     8281 ggtcatcgtc gtcctgctct atttcttcat cacctcggga atcacccggc cgctgcggga
     8341 aatggcagcg gcgaccggga agattgccga gggggacctt tccatcgaac tctccagcaa
     8401 gtcgaaggac gaaatcggcc agctggctca atccttcaat tatatgctgg tgcgtttaaa
     8461 acaggcccgt aaggagctgg aggattacgg gcgggtcctg gaggtgaagg tcgaagagcg
     8521 aacccaggag ctgaccaaaa tgcaggacca cctcatccag tcggaaaaaa tggcctccat
     8581 gggccagctg gccgcttcca tcgcccacga ggtgaataat cccctcagcg gggtcctcat
     8641 ttacaaccag ctcatcgcca agaaaatcac ccggggggat tttgaccggg aggtgatcct
     8701 ggattacctc tccaagatgg aacatgagct ttcccgcagc accaaactgg tacgcagcct
     8761 gctggacttt ggggttcaaa ccgagccgaa aatcaaagag gtcaacgtca acgaggtctt
     8821 gaaccgggcc ctggaactgg ggattcatgc cggatacaag gagaacaccc gggtggaaaa
     8881 ggacttgcgc cctctaccta ccttcatgga ggacccagac cagctggaac aggtcttcgt
     8941 gaacctcatc atgaatgccc tccaggccat gcccacggga gggaagctga ccctgcgcac
     9001 gttcatggag aatcgggagg tgaaaatcgc cgtgcaggac acaggttgcg ggattccccc
     9061 cgaaaacctg agtaaaatct tcaccccctt cttcagcacc aaaaaagagg taaagggagt
     9121 cgggctgggg ctgtcagtgg cctatggaat catccaggga cttaaaggaa ggatcgaagt
     9181 ggaaagtaaa gtgggagaag gaacgacctt taccgtctgc ctgccgatca cccaggccta
     9241 gggcccaccg aaaaaatcct gatcatcctt acattcctcg agagccagac ggtaaaaccc
     9301 ttcgaatctt ggcaggcata tttggctggg aagcgcgtct ctgggatcac ccgtaaccct
     9361 tgattccaga agggtgagct gcttttggtt gcggccgcgc cgcgctgtga ttccaggaag
     9421 aaaatcctcc ttggtgggac cgcttgcaat gatcaggcaa cgatcgctcc ctgcctggag
     9481 cctgcgtcgt aatcccccct ttataccttc ttgatattca ccgcgcagac cttgagcccg
     9541 gggatcttgg ccaccgggtc cagggcgtcc agggtgagca ggttcaccgc cgcttccttg
     9601 aagtggaagg tcatgaagac cactcctgga gggagcgctt ccgag
//
LOCUS       DASPRR010000046         7696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_15120, whole genome shotgun sequence.
ACCESSION   DASPRR010000046 DASPRR010000000
VERSION     DASPRR010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15120"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1693)
                     /locus_tag="VEL68_01770"
     CDS             complement(50..1693)
                     /locus_tag="VEL68_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ34726.1"
                     /translation="MLIHQLTGEEALAQGAWEAGVCVATGYPGSPSTRFLERLAEISR
                     IGDVYCEWSANEKVAFEMALGASMAGKRASVSMKSVGLNVALDPLMVANLGGVSGGLV
                     IILGDDPGAWGSQNEQDGRLLVRAAEVPLLEFSSPQEAYDMVQYAFSLSEKFELPVFV
                     RETRSSASEKGAVYVKEARVQTLQKHFLELEPWKCFPENPPEKHRKLHAKIQKITNEF
                     NQCPFNHVLLKSSKGIVAAGFMAARAGRLLSGKGGEQLSFFKLGTIYPPPEASLIKFL
                     SQMEEIMVAEELQPLVEGYLRSLAHLHNLKTHIYGKETGHLPKYGELFESHLRRSVEE
                     SLKVEIGLPEPSNPERDEKNKVLYKSLPPECPFFKAFEAFSIVISNNTKMRPIFVGDD
                     GCLTRLRNAPFSLLGCKFNMGSSIGIAAGLYWAGEKRKMVVLVGDSSFFHTALPAYLD
                     AVATGVRMMVVVLDNRCTAMTGCQANPGTDWALQKRAQKSISISSILQSPGIPLFREV
                     NAFGDPKDMAAAFQECFQSEDFAVLLVTGPCPNLKEKVC"
     gene            complement(1926..2501)
                     /locus_tag="VEL68_01775"
     CDS             complement(1926..2501)
                     /locus_tag="VEL68_01775"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXZ34727.1"
                     /translation="MNKKEIEPTVLFFPNPVVLVTSQGVESKPNIITIAWTGVVNSRP
                     PMIGISIQPIRHSHGLIRQSGEFGVNIPNRDLLRAIDYCGFVSGKNTDKFAATGLTPV
                     LARKIKAPLIKECPVNLECVLRHFITLGMHDLFIGEIVSIYVDEQVLDDKGKLDSSLL
                     NPVVYFSQPTDYWSKGERVGIRGFTKGEYRR"
     gene            3019..6561
                     /locus_tag="VEL68_01780"
     CDS             3019..6561
                     /locus_tag="VEL68_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ34728.1"
                     /translation="MQATTICLRCHQKCHLVAEVIDGKIVSMVDAQASNRRPACREAC
                     PIGMDVSGYVIAASKGEFDKAMQIIWDTNPFPLVCGRICHHPCEIECIRGVVDEPIAI
                     ASIKRMVADHVLKSRTKAMPARRTQKETVGIVGSGPTGLTAAHDLVKAGYGVTVYEGA
                     PEAGGMMTRVIPEFKLPKGSVQPDIEAIRDLGVEIKTNTPVGKTLPLGSLLKRHNAVL
                     LAIGSWTAAALKIPGTELQGVYFALPLLEEIKKGKPVALGKRVVVIGGGNTAMDVARA
                     AIRLGAKEVNITCLESRKTMPAHPWEIENALREGAKVHPSLAPQKFQEKGKGKVGGVD
                     FKQVETCQVDSYGNLTWTLRECAGSELSMNADSVLIAIGQVPSLESLSVNGGLTLTSR
                     KTLAVDPETLACGIPGLFAAGDAVVGAGTIVEGMAAGRKAAGSIIKYFTGSEPRNEEA
                     PIEETLQRVRQSVNEDFPKERQRQVMPTLPSREAVSSFQEVDRGFAEEAGRKEAARCL
                     NCATVCIKGATIPEVMYHPDRVKYPIQRVGARGEGKWQRISWDEALDTIAQNLKRIKE
                     KYGPEAIHVSCGSGQKHIGIQATKIAERLWPTPNTHLGRYTCIHPDVMANGATFGDTI
                     TYEFGIDYLDAHCIIFWGAEPDVATPAQARVVHRALRRGAKLIVVDPRPIPMAKRADV
                     WLRIRPGTDMALALAMQHVIINEGIYNREFVEKYCYGFDKLEEHVQKYTPEWAEKVTS
                     VSREDIVKAARLYALERPGCIYIRLGSGAQQVTSTQTCRSISILIGITGNADLKGSNQ
                     LYYRTFREALMWHPYLMFWGVKPPAAVNEKRIGAKDYPLMHKRAICHVPSTIQAMEEG
                     RVRAMWAVADNLIVAEMDNRRLWDILKNKLDFFFVSELFMTPTAELADIVLPTAFYPE
                     IDQLTEAFGHPASTVTATKKVVEPIGECRDDREVAIDLAKRMGMDVAPWETLHDYFNW
                     MLKYQGITFQELLRKPNGTLTFPRGYERYRTSTPPFSTPTGKVELYSPTFEAMGVDPM
                     PVYEEPPESPVRTPELFKKFPFIYTHYRIHGYMHSEGRQIKRQRQLAPEPYLQMNPER
                     AAGLGLAEGDWVYLETPKSAGKNHLKYRLQLIPDMHPDVVAGPHAWWFPEKAEPEHGC
                     FESNINALVPLDPPYDPVVGVPQVRAILCRVWKA"
     gene            6731..7300
                     /locus_tag="VEL68_01785"
     CDS             6731..7300
                     /locus_tag="VEL68_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34729.1"
                     /translation="MSSGIRETMEELLRASAEVKNKLAGALEEISQYGIANALKENPD
                     FLYRLLIQLRKADASRVLVQVSKAADKFSDLLWQWIDTQSQQSQSMKSLLIKAQRDIH
                     VNIEASDSPFQCHFMVKGGKITGGSGLLHFKDEDFRFMGPTETLMGLFIGDLPLGFSN
                     LQLQTAGHSGWMSRVGPIMREISKLLKGS"
BASE COUNT         1915 a         1974 c         2083 g         1724 t
ORIGIN      
        1 caaggttggg gagatctacg aaggcaaatt atcggaagag aaagggatct cagcagacct
       61 tttctttaag gtttgggcag gggccggtta ccaatagaac cgcgaaatcc tcgctttgaa
      121 aacattcttg aaaagcagcc gccatatctt taggatcacc gaaagcattg acctcacgga
      181 acagagggat tccgggagat tgcaaaatag atgagattga aatactcttc tgcgctctct
      241 tctgtaaagc ccaatccgtt ccaggattgg cctggcagcc tgtcatggcc gtgcatcggt
      301 tgtccaggac cacaaccatc atccggaccc cggtagccac cgcatccaga taagcaggaa
      361 gggccgtatg gaaaaaagaa gagtccccta caaggaccac catcttccgc ttctctccag
      421 cccaatagag ccccgcagcg atcccgatgc ttgaacccat gttgaatttg cagccgagca
      481 gagaaaaggg cgcatttcgc agccgggtga gacagccatc atccccaaca aaaataggcc
      541 gcatctttgt attattggaa atcacgatgg aaaaagcctc aaaggcctta aaaaatgggc
      601 actccggagg caaactctta tagagaactt tattcttctc atccctttcg ggatttgagg
      661 gttccggaag gccaatctca accttcaggg attcctccac cgatcggcgg agatggcttt
      721 cgaaaagctc gccgtatttg ggtagatggc ctgtttcctt tccataaatg tgagtcttca
      781 gattgtgcag gtgggccagg gaacggagat aaccttcgac taggggttga agttcctccg
      841 ccaccatgat ttcctccatt tgtgaaagga atttgatcaa agatgcttcc ggaggtgggt
      901 agatcgttcc cagcttgaag aagctcaatt gctctccgcc tttaccactc aacaaacgcc
      961 cggctctcgc cgccataaat ccggcagcca cgattccctt gcttgatttc aaaagcacgt
     1021 gattgaaagg gcactgattg aattcattcg tgatcttttg aattttggca tggagttttc
     1081 ggtgtttttc cggcgggttt tcggggaaac atttccaggg ttccagttcc aggaaatgtt
     1141 tctggagggt ttgaacccgc gcttccttta cataaacggc ccctttctcg ctggcgctgg
     1201 accgagtctc tcggacaaaa acaggcagct caaatttctc cgaaagggaa aaggcatatt
     1261 gaaccatgtc ataggcttcc tggggagagc tgaattcgag tagaggaacc tctgctgctc
     1321 ggaccagtag acggccgtcc tgttcattct gcgatcccca agccccggga tcatccccca
     1381 ggatgataac caggccaccc gatactcctc cgagattcgc caccattaag ggatccaggg
     1441 ccacattgag cccaacactc ttcatggaaa cggaggcccg ttttccggcc atcgaggccc
     1501 ccagagccat ttcaaaagcc accttctcat tggccgacca ttcgcagtaa acatctccga
     1561 tccgggaaat ctctgccagc cgctccaaga aacgagtaga tggtgatccc ggataacctg
     1621 tagcgacgca aacgcctgct tcccaggccc cttgggccaa agcttcttct cccgtcaact
     1681 gatgaatcaa cataattttt ctcccatggg tgagggtatg aattctttca gcataaggtc
     1741 atcgtcaacc aatttttgca gaggtccaac attagggcgt ttcctgcacg cctcagctct
     1801 taggagcgaa gcactctgtg tttcgatttt ttccacagcc ataatgagac ctggttataa
     1861 tcattttggg gtctcgcccc cattgcctgt tttctgcagg ctggcgtggg cgttattttt
     1921 gtccttcacc tccgatattc ccccttcgta aatccgcgaa tcccaaccct ttcccctttc
     1981 gaccagtaat cggtgggctg ggaaaaataa acgaccggat taaggaggct cgaatcaagc
     2041 ttgcccttgt catcgaggac ctgttcatcc acataaatgc tcacgatttc cccaatgaat
     2101 aagtcgtgca tcccgagagt gatgaaatgc ctcaatacgc attcgagatt gaccggacac
     2161 tctttgatca aaggagcttt gattttccga gccaataccg gggttaaccc tgttgccgcg
     2221 aatttgtccg tattcttccc ggatacaaaa ccacagtaat cgatggcgcg caggaggtcc
     2281 cgattcggga tattcacccc gaattccccg gattggcgaa tcaaaccatg ggagtgacga
     2341 ataggctgaa tggagatccc gatcatggga ggtctggagt taaccacgcc ggtccaggca
     2401 atcgtgataa tattaggctt cgattccacc ccctgggagg tgactagaac aacgggattg
     2461 gggaaaaata aaactgtcgg ctcgatttcc ttcttgttca tataatcctc cttggaaaga
     2521 aattcaaatt ctaaaggata tagtatattc ttgtttatac tgaccatggg aaagaaaggt
     2581 aagatgcttt taatggtgca aattagatta aaatttcata tttgtcaacg aacgaattct
     2641 gaaagaagaa aaaatgagtt ttgaaaaatc tctcctagtg atcttatcta aattttggag
     2701 ggatcattta ggttattttc ttctgcaaaa attgcttgca gacgggagga aaggaatttg
     2761 ggcttttcag tacccctaaa ataccatgga cttcggcctg ttcggtgaaa aaattttaca
     2821 aattggtctc ttttctcttg aaataaaaaa tatttttgat ataagttaaa tccttgagaa
     2881 ataatctatc cgggaattta tgaatgggca aagttgctaa cagaacgctt cagattctat
     2941 caaagttttc tattgataaa ccgtgtttaa ccgcattttt tcaatgccaa tcaagaatat
     3001 ttgaaaggat ggttttgaat gcaagcaacc acgatttgtc ttaggtgcca ccaaaagtgc
     3061 caccttgtag ctgaggtgat cgatgggaaa atcgtttcga tggtcgatgc gcaagcctcc
     3121 aaccggaggc ctgcctgccg cgaagcctgc ccgatcggga tggacgtttc gggatatgtc
     3181 atcgcggcct caaaagggga attcgataaa gccatgcaga ttatctggga tacgaatccc
     3241 tttcccctgg tatgcggccg gatttgtcac cacccctgcg agattgaatg catccggggg
     3301 gtcgtcgatg aacccatcgc gattgcctcg atcaaacgaa tggttgccga tcacgtctta
     3361 aaatccagga ctaaggcgat gccggctcgt cgtacccaga aggaaaccgt gggtatcgtc
     3421 ggctccggac cgacgggtct tacggccgct catgacctgg tgaaggcagg ttacggggtg
     3481 accgtgtacg aaggggctcc ggaagcgggc ggcatgatga cccgggtcat ccccgaattc
     3541 aaactgccaa agggcagcgt tcagcccgat atcgaagcca ttcgagacct cggggtggaa
     3601 atcaaaacga ataccccggt agggaagacg ctgccgctgg gaagcctctt aaaaagacat
     3661 aacgcggttc tgctggccat cgggagttgg actgcggcgg ccctgaagat cccgggaacg
     3721 gaactgcaag gggtctattt cgcccttcca ctgctggagg agatcaagaa aggaaaaccg
     3781 gtcgccctgg gaaagcgggt cgtcgtgatc ggcggtggaa ataccgccat ggatgttgcg
     3841 cgggccgcga tccgccttgg cgcgaaggaa gttaatataa cctgcctgga gtcccggaag
     3901 accatgccgg ctcatccctg ggagatcgag aatgctttgc gggaaggagc gaaggtccac
     3961 ccatccctgg ccccgcaaaa attccaagaa aagggaaaag ggaaggtggg gggcgtcgat
     4021 tttaagcagg tggaaacctg tcaggtggac agttacggaa atctcacctg gacccttagg
     4081 gagtgcgcgg ggagcgagct ttccatgaat gcggactccg tgctcattgc tatagggcag
     4141 gttccctccc ttgagtcctt gagtgtgaat ggagggctca cgctcacctc ccgcaaaact
     4201 ctggccgtgg acccagagac cctggcctgc gggattcccg ggctctttgc tgcgggggat
     4261 gcagtggtgg gggcggggac gatagtggag ggcatggcgg ccggtcggaa ggccgcgggc
     4321 tccatcatca aatacttcac cggttccgag ccccggaacg aggaagcgcc catcgaggaa
     4381 accctccagc gggtccggca aagcgtcaat gaagattttc cgaaagaacg gcagcggcaa
     4441 gtcatgccca cccttccgtc ccgggaagcg gtttcctctt ttcaggaagt cgatcgggga
     4501 ttcgccgaag aggcgggccg gaaagaagcg gcccgctgcc tcaactgcgc gacggtctgc
     4561 attaagggag ccacgattcc cgaggtcatg taccaccccg atcgcgtaaa atatcctatc
     4621 cagagggtcg gagcgcgagg ggaaggaaag tggcagagga tttcctggga cgaggcgctg
     4681 gataccattg cccagaactt gaagaggatc aaggagaagt acgggccgga ggcgatccat
     4741 gtcagctgcg ggtccggcca gaaacatatc gggatccagg cgaccaagat tgcggagcgg
     4801 ctctggccta cgcccaacac tcatctgggc cgctatacat gcatccatcc ggacgtcatg
     4861 gccaatggcg ccacgttcgg ggacacgatc acctatgagt tcgggatcga ttaccttgat
     4921 gcccattgca ttatcttctg gggggccgag ccggatgtgg ccactccagc ccaggcccgg
     4981 gtcgtgcacc gcgccctgcg ccggggggcc aaactgattg tggtcgatcc ccggccgatt
     5041 cccatggcca agcgggccga tgtgtggctt cgaatccggc cgggaacgga tatggccctg
     5101 gcactggcca tgcagcatgt gattatcaac gaagggatct ataaccgaga gttcgtcgag
     5161 aagtattgtt acgggttcga caagctcgaa gagcacgtcc agaaatacac ccctgagtgg
     5221 gcggaaaagg ttacgagcgt ttcccgggaa gatatcgtga aagccgcccg gctctacgcc
     5281 ctcgaaaggc ctggatgcat ctatatccgc ctgggctccg gggcccagca agtgacctct
     5341 acccagacct gccggtctat ttctatcctc atcggaatta cgggcaatgc ggatttaaag
     5401 gggagcaacc agctttacta ccggaccttc cgggaagcac tgatgtggca tccctatctc
     5461 atgttctggg gcgtgaaacc tccggccgcg gtcaatgaaa aacgaatcgg agccaaggac
     5521 tatccgttaa tgcacaagcg agccatctgc catgtgccaa gcaccatcca agccatggaa
     5581 gaagggaggg tcagagccat gtgggccgtc gccgataacc tgatcgtcgc cgagatggat
     5641 aaccgaaggc tctgggacat cctgaagaac aagctcgatt tctttttcgt gtccgagctc
     5701 ttcatgaccc cgacggccga actggcggat atcgtcctcc ccacggcttt ctacccggaa
     5761 atcgatcagc tcaccgaagc cttcggacat cccgccagca cggtcacggc cacgaagaag
     5821 gtcgtcgagc cgatcggcga atgccgggac gaccgggagg tagccatcga ccttgcgaaa
     5881 cggatgggaa tggatgtcgc tccttgggag acgctccacg actacttcaa ctggatgctg
     5941 aaataccagg gcatcacctt ccaggagctc cttcggaaac ccaacggcac gttgactttt
     6001 ccgcggggct atgaacgata ccgtaccagc acccctcctt ttagcacgcc caccgggaaa
     6061 gtggagcttt attccccaac gttcgaagcg atgggggtcg atcccatgcc ggtttacgag
     6121 gagcccccgg aaagccccgt gcgcacccca gagcttttca agaaattccc cttcatttac
     6181 acccactacc ggatccatgg gtatatgcac agcgagggcc ggcagatcaa acggcagcgg
     6241 cagctggctc cggagcctta cctgcagatg aatccggaaa gggctgccgg gttaggtctg
     6301 gccgaaggag actgggttta tctggagacg cccaaatcgg ccgggaaaaa tcatttgaaa
     6361 tatcggctgc agctcatacc ggacatgcat cccgatgtcg tggccggccc tcatgcatgg
     6421 tggttccccg agaaggctga accggagcat gggtgctttg agtccaatat caacgccctc
     6481 gtgcctctcg atccgcccta tgacccggtg gtgggtgtcc cgcaggtgcg ggcgatcctt
     6541 tgccgggtgt ggaaggcctg accaagagca gcgctcagtt atcagccgtc ggcgtccgaa
     6601 aaaggaagag gtccaagatc tggaggaata aaaatatcta gaagccggaa gaaaagaatg
     6661 aggcttaagg ggagaaaaaa atcccctgat ggctgaatcc tgattgctga ctgtttcata
     6721 gggaggaaaa atgtcaagcg ggataaggga aaccatggaa gagcttttgc gcgcgtctgc
     6781 cgaggtcaag aataaattgg ccggagctct ggaagagatt agccaatacg ggatcgcgaa
     6841 cgcgttgaag gagaaccctg attttttgta ccggctcttg atccagctga gaaaagcgga
     6901 tgcctccaga gtgcttgttc aagtttccaa agctgcggat aaattttcgg atcttctctg
     6961 gcagtggatc gatacccagt cgcaacaatc gcaaagcatg aaatccttgt tgataaaagc
     7021 ccaaagggat atccatgtca atatcgaggc atccgatagt ccctttcaat gtcatttcat
     7081 ggttaaaggg ggaaagatca ctggaggctc tgggctgctt cacttcaagg atgaggattt
     7141 tcgttttatg ggtccgacgg agactttaat ggggctgttc atcggggacc tgcctttggg
     7201 tttcagcaat ctgcaactcc agacggcagg gcattccggg tggatgagcc gagtaggtcc
     7261 gatcatgcgc gagattagca agcttttaaa aggaagctga agggctaccg taagaagccg
     7321 caagccggcg gcgatcatcg atccgctgga tcgcaaaatg aaaagccccg ataggctggg
     7381 attctttcat catgagtctt taaaaagcct gccgcacagg aatttggtgg tctaaaatcc
     7441 gattctgcgg cttgggtcct tagcttccat catatggaac cagggggaag aaatagttga
     7501 acatcttttg gaggttcacc tgttttcctc attaagaatt gagcaacagg catttaccac
     7561 ttggggtaca gtcaaaagac taatcaaaaa ctcaaaagga gggaaagatg aaaaaggtaa
     7621 tgttcttcat catcggtttc ttcgtttgta cccttgtttt gctcccggga aatgggtgga
     7681 cccaggataa gaaacc
//
LOCUS       DASPRR010000047         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_662717, whole genome shotgun sequence.
ACCESSION   DASPRR010000047 DASPRR010000000
VERSION     DASPRR010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662717"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1312
                     /locus_tag="VEL68_01790"
     CDS             212..1312
                     /locus_tag="VEL68_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HXZ34730.1"
                     /translation="MSAEFKTVWAKPLTEFCQRVFEKLGMPPEDGFTTADVLVLADLR
                     GIDSHGVARLRRYFTGLKNGMMVARPQMKVAHETPLTALLDGGAALGQVAGKRGMEMA
                     IEKALKNGVGFVAVRNSNHYGIAAYYSLMALKHDLIGISLTNSDAYVVPTFGKEVMLG
                     TNPISVTVPAMEERPFVLDMSTAVATLGKFEVYSRMGKKIPLGWATDEKGRSSDDPTR
                     VIANIRSKAGGGILPMGGEGEESGGHKGYGLDLLVDILCGVLSGSGYLNLLYPKTPDG
                     KPLPSKVAHFFGALRVDYFRPVQEFKKDMDDLIRRLRNSAKAEGQTRIFIHGEKEFEL
                     EEKYRKEGIPLYFKVYDDLKAIASELGVKFDL"
     gene            1500..2627
                     /locus_tag="VEL68_01795"
     CDS             1500..2627
                     /locus_tag="VEL68_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HXZ34731.1"
                     /translation="MITLPAHKVRQFGVEFYQTSFTSGDIQKLVKFEVLSYAAPEEGQ
                     KKSRSAKTSPVNWGLLEKRIGQSEAAFQRPMIRKKIRELVQYYAECQEAQNLPAIPGA
                     VIMITEKRVDFSPMARNPNLGMIQIPEEPGILRCLDGQHRLLALSSQEEKNAEIDVPA
                     VLFDSLDPRQTVELFVTINAKHTRLNPSHLVSLAGRRLYADPHQALAHDIIRQLNEEE
                     DSPLQGDIKILGVGKGRVSQASLADEMVDLFGTIEKVGGPNRFKEFEQNGKRFFMNYF
                     KGIQVTFPKAWAGRKYSIKTGAALRAFIRVGPDVMARSRELKNDPFDHFGIREAIKPW
                     GERMGEGRFETDGEWRRKLAGGTRGTVELLTRELQDAMKAP"
BASE COUNT          613 a          678 c          806 g          536 t
ORIGIN      
        1 gaggggatgt accgaatttc gctcccatat ctccttttgg aacacggtct ctgagatgag
       61 ggagcgggtt catgaatttt tccagaattt gagaacgctt cctcttttct cttgacttag
      121 ctctccttat cctttaaatt ctttttccag cgattctaat cgcgaggatg aagtttcccc
      181 catgcatcgc cctgtggaga ggaaggaatc tatgagcgca gaattcaaaa ccgtatgggc
      241 aaagccccta acggaattct gtcagcgggt ttttgagaaa ctggggatgc ctcccgagga
      301 cggcttcacc actgcggacg ttttggtgct ggccgacctg cggggcatcg actcccacgg
      361 cgtcgcccgg ctgcgacggt attttacagg cttgaagaac ggcatgatgg tggccaggcc
      421 gcagatgaaa gtggcccatg agacccccct aaccgccctt ctcgacggcg gggcggccct
      481 gggccaggtg gccgggaagc ggggtatgga gatggccatc gagaaggcgc tgaagaacgg
      541 ggtggggttt gttgccgtgc ggaacagcaa ccattatggg atcgccgcct attattctct
      601 gatggccctg aaacatgacc tcatcgggat ctccctgacg aactccgacg cctatgtcgt
      661 ccccaccttt ggtaaggagg tcatgctggg aaccaacccc atcagcgtga cggtccctgc
      721 gatggaggag cggccttttg ttctggacat gtccacggcg gtggccacgc tggggaagtt
      781 cgaagtttac agccgcatgg gcaagaagat ccccctggga tgggccaccg acgagaaggg
      841 acggtccagc gacgacccga cgcgggtcat tgccaatatc cgcagcaaag ccgggggagg
      901 gatcctgccc atgggtgggg agggggaaga atccggcggc cacaagggat acgggttgga
      961 cctgttagtg gacattttgt gcggagtcct ttcggggtcc gggtatttga acctcctcta
     1021 cccgaaaacc ccggacggaa agccgctgcc ttccaaggtc gctcattttt ttggggctct
     1081 gcgagtcgat tacttccgcc cggtgcagga atttaagaag gatatggacg acctcatccg
     1141 ccggttgagg aactcggcca aggccgaggg gcagacccgg atctttatcc acggcgaaaa
     1201 ggaattcgag ctggaagaaa aataccgcaa agagggcatc cccctttact tcaaggtgta
     1261 cgatgacctg aaggccatcg cgtccgagtt gggagtgaaa ttcgatttat aagatcgatt
     1321 cgggaattgt tttggtggat caccgcaccc gcgattcgcg aggtctcaag gagtccagat
     1381 ggctggaaac cgaaaacccc atctcttata ataaagggat ggggcctttt ttttgatgag
     1441 gaagcgggac tggttttgga atgcacggtt aggaaaaaat tcggaaggag gtggggtgaa
     1501 tgattactct tccagcccat aaggtcaggc aatttggagt ggagttctat caaacctctt
     1561 ttacgtcggg agacatccag aagctggtca agttcgaagt gttgagttat gcagccccgg
     1621 aggagggcca aaagaaaagc agatcggcta aaacctcgcc cgtaaactgg ggattgttag
     1681 aaaaacggat cggacagagc gaagccgctt tccaaagacc gatgattcgg aagaagattc
     1741 gggagctggt ccagtattat gcagaatgcc aggaagctca aaatctgccg gccattcccg
     1801 gcgccgtgat catgatcacg gagaaaaggg tcgacttttc accgatggcg aggaatccca
     1861 acctggggat gatccagatt ccggaggagc cgggaatcct gcgatgcctg gacggccagc
     1921 accgcctgtt agccctgagc tcccaggagg agaagaatgc ggagatcgat gtccctgccg
     1981 ttctcttcga cagcctggac ccgcggcaaa cggtagaact ctttgtcacc atcaatgcca
     2041 aacatacccg gctgaatcct tctcacctgg tctctctagc cggccggaga ctctatgccg
     2101 acccgcatca ggccctggcc catgacatca ttcgtcagct caacgaagaa gaagactctc
     2161 cattgcaggg ggacatcaaa atactcggtg tgggcaaagg ccgggtctcc caggcctcgc
     2221 tggccgacga gatggtggat ctcttcggta cgatcgaaaa ggtgggcggg cccaaccgct
     2281 tcaaggaatt cgagcagaac gggaagcggt tcttcatgaa ctattttaag ggaatccagg
     2341 tgaccttccc gaaggcctgg gccggtcgaa aatacagcat caagaccgga gccgccctcc
     2401 gggccttcat ccgggtgggt cccgatgtga tggcgaggtc ccgggaactg aagaacgacc
     2461 ccttcgacca cttcggaatc cgcgaggcga tcaaaccctg gggcgagagg atgggcgaag
     2521 gaaggtttga gaccgatgga gagtggcgga ggaagctggc cggggggaca cggggaaccg
     2581 tggagcttct cacccgggag ctgcaggatg caatgaaagc cccatgaata ggg
//
LOCUS       DASPRR010000048         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_816778, whole genome shotgun sequence.
ACCESSION   DASPRR010000048 DASPRR010000000
VERSION     DASPRR010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816778"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VEL68_01800"
     CDS             <1..549
                     /locus_tag="VEL68_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ34732.1"
                     /translation="GDSTFYHAGLPPLVNAVHNGAKFILVLLDNSTTAMTGNQPTPGM
                     EVLVDGRKGKAVSMEELVKASGVKSLSVVDPYSLEEMTRALKEADKVCRSEEGGVAAI
                     ITRHPCLMDLGLERKKRTERVEISEDCIGCRVCIDDFECPAVEADPETGLARINQTIC
                     SACGVCIQVCPQNAIKRGRSEA"
     gene            657..1175
                     /locus_tag="VEL68_01805"
     CDS             657..1175
                     /locus_tag="VEL68_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ34733.1"
                     /translation="MDMQIAITGRGGQGVLFLTRILSECALEMGLEVIASETHGMAMR
                     GGSVISTLKVGAFRGPLIGSGQADMMLVLDPGSYDTFANLLSPKGIAFLNAASSNDHP
                     HIDATALAEKMGSPLMSNLVLLGFALGHDRLFCKYDQVEEMAGKISPPRFKEANLQAL
                     RLGYSASQKEKA"
     gene            1172..2056
                     /locus_tag="VEL68_01810"
     CDS             1172..2056
                     /locus_tag="VEL68_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34734.1"
                     /translation="MSLWLQIFQFTLSGLTTGSTYALVAIGFSLIHNATGIVNFAQGE
                     FVMLGGMFMITFYSFLRLPMVPAFVLTIFCVAAIGFLLERGPINRARSREILILVMIT
                     VGASISIKGLSMMIWGKNSMTLPPFSGENPLIFLGAAIMPQSIWIFGITLLVVIALSY
                     FFRGTMAGKAMRAVAASRRTALLSGIPVDRMILLSFTLSGALGAVAGIILTPITTTSY
                     DVGVMMGLKGFSAAILGGYGSMPGAVIGGLLLGVLESLGAGLISSEYKDALAFLILLI
                     VLFFKPTGILGKGSFKRV"
BASE COUNT          440 a          607 c          667 g          501 t
ORIGIN      
        1 ggggattcca ctttctacca tgccggcctg ccgccgctgg tcaacgcggt tcacaatggg
       61 gcgaaattca ttctggtgct ccttgacaat tccaccactg ccatgaccgg caaccagccg
      121 acgcccggga tggaggtcct agtggacggc cgcaaaggaa aagccgtttc catggaggaa
      181 ctggtgaaag ccagcggggt gaaatccctg tcggtagttg atccgtactc tctcgaagaa
      241 atgacccggg ctctgaaaga ggcggacaag gtttgccggt ccgaagaggg cggcgtggcg
      301 gccatcatca cccggcaccc ctgcctgatg gacttagggc tggaaaggaa aaagagaacg
      361 gagcgggtgg aaatatcgga agactgcatc ggctgccggg tctgcatcga tgattttgaa
      421 tgcccggcag tcgaggcgga cccggaaacg ggcctggccc ggatcaacca gacgatctgc
      481 tcggcctgcg gcgtctgcat ccaggtctgc ccgcagaacg caataaaaag agggaggagt
      541 gaggcgtaag ggctgagggg taaggaatcg ggaagtgaga gttgggagtt gaaagttgca
      601 aaaaaggggg ttacaactcc cacctctcag ctctcaactc acttggaggg gttttcatgg
      661 acatgcaaat tgccatcact ggaaggggag ggcagggggt ccttttcctg acccggattc
      721 tcagcgaatg cgccctggag atgggactgg aggtcattgc ctcggaaacc cacgggatgg
      781 ccatgcgggg agggtcggtg atctccaccc tgaaggtggg cgctttccgc ggccccctga
      841 ttgggagcgg ccaggccgat atgatgctgg tattggaccc ggggtcctat gacaccttcg
      901 ccaacctcct ctccccaaag ggaattgctt ttctgaacgc ggcgtcctcg aacgatcacc
      961 cccacatcga tgccaccgcc ctggcggaga agatggggtc gccgctcatg tccaacctgg
     1021 tgcttttggg ctttgccctg ggccatgacc ggctcttctg caaatatgac caggtggaag
     1081 aaatggccgg gaagatatct ccaccccgtt tcaaggaggc caatctccag gctttaagac
     1141 ttggctattc cgcgagtcag aaagagaaag cgtgagtctc tggctgcaga tatttcagtt
     1201 caccctctcc ggtctgacca ccggcagcac ctatgccctg gtggccatcg gcttttccct
     1261 gatccacaat gccacgggaa tcgtcaattt cgcccaggga gagttcgtca tgctgggggg
     1321 gatgttcatg atcaccttct acagcttcct gcggcttccc atggtcccgg ccttcgtcct
     1381 gaccattttt tgcgtggcgg ccatcggctt tctgctggaa cgaggaccga tcaaccgggc
     1441 tcgttccagg gaaattctca tcctggttat gatcacggtg ggtgcttcca tctccatcaa
     1501 gggcctgtcc atgatgatct gggggaaaaa ctccatgacc ctgcctccct tttccggaga
     1561 aaatcccttg atcttcctgg gggcggcgat catgccccag agcatctgga tctttggaat
     1621 tacgctcctc gtggtcatcg ccctcagcta cttcttccgg ggaacgatgg ccgggaaggc
     1681 tatgcgggcg gtcgcggcca gccggagaac ggctcttcta agcggaattc cggtggaccg
     1741 gatgattctc ctttccttta ctctcagcgg ggcgctggga gcggtagctg gaatcatcct
     1801 gacgcccatc accaccacct cttacgacgt gggggtgatg atgggactga agggcttttc
     1861 cgcggccatc ctgggagggt acgggtccat gcccggagcg gttatcgggg gtctcctcct
     1921 gggagtgctg gagtcgctgg gcgcgggcct gatctcttcg gaatacaagg acgctttggc
     1981 ctttttgatt ctattgatcg ttttgttctt caaacccacg gggattctgg ggaagggaag
     2041 ttttaagcgg gtgtgaggct tggagatggg aaaacagaat caaatattcc ttcttctttt
     2101 cacctcggtg gtttttgctc tgcccttttt ccttaggaac aactaccatc tgatggtgct
     2161 gaatatttcc gccctgaaca tcctggtggt catcgggctc aacctcctca tgggc
//
LOCUS       DASPRR010000049         4492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1948090, whole genome shotgun sequence.
ACCESSION   DASPRR010000049 DASPRR010000000
VERSION     DASPRR010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948090"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1253
                     /locus_tag="VEL68_01815"
     CDS             270..1253
                     /locus_tag="VEL68_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34735.1"
                     /translation="MSGKKALWILVIFCSAGLAVPGEHFAAEKKFPTKPINVIIPFQP
                     GDTDNNLRPFTDKMVQYLGQPVNFVYKPGASGAVGAGFVATAEPDGYTLVGSQQSSLV
                     LVPLTQKGLTYSLKNFSPVCGLAGGYNIIGVQTGARWKNIQELIAEAKQNPGKISYVS
                     GSGALGITTLIAEAFFKEAGVKLNLIPAQGSGPAVTAILGGHTDAVSSQITPAFPHIQ
                     AGTLRALVVSTEKRVPALPDTPTAVELGYKVSVPSLYGLLAPKGTPKEVVDKIALAAK
                     KAAEEHRGAIEASLSKAGMQILYAGPEGFAQFLKSQDDYWTQTVKTLDLKM"
     gene            1366..2382
                     /locus_tag="VEL68_01820"
     CDS             1366..2382
                     /locus_tag="VEL68_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019382234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HXZ34736.1"
                     /translation="MPEKTIQVKDVLAALQQITGGRVIITAENAFAAGSPFVVLKTSH
                     IPGKAVMETPGLVCGDPQKEVRRLAVSMTLTESQIELAGGTGVDAIVAHHPVADAASC
                     GGVPLRGYLNLYNLAVFEVHEAFHGLHPGIALIHGHKPFRVEIAYGGLSGNVLFVGRP
                     LPEIKKAGDILNRLELFSDLAGEKGFLRLEQDQRECPELMETSIAVAPEVLNGSENDP
                     VGTVLHFFPHTGFTPDHLRSAKREHPEITTAIVSISRVKKDHPLVNTARALGLTMLAG
                     NSHALEILENGIPLAYALKTLLPGVEVVLFRERVTSVPLEKIGSKVMRDFAGEMAAHH
                     LLKR"
     gene            complement(2477..3949)
                     /locus_tag="VEL68_01825"
     CDS             complement(2477..3949)
                     /locus_tag="VEL68_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXZ34737.1"
                     /translation="MSPTLVIVGVGLYTILMFAIGFWGYGKSRPSMEDFFLGGRTLGL
                     FVVLCTVWSTLFSAYSYIGLPGAYYRTGISFFGVAGNILLNSLCMYLLGSRMWVLGKK
                     HGFINMTDLFAERYGSNLVCVIAMIISIGSLLPYIGLQIKGAGLTLQGVTNNVISFNM
                     GLLYITLVVLFYVLLGGFRSVVWTDVVQSIIMLVGMLGAMFLIADKVAGGLGGVIEKA
                     SAISPKLVSFPGPAGVWTPSMVLTTAMVVGLGGFAWPQISQRMYATKRLRTIKMLAFI
                     FPLAALFVNLPPIFIGLAGRVQYPDLKNPDLVFPLMLNELVPPVFGIIIVLAILAAIM
                     STVSGQILSLTSIIVRDFYVRFFDPAAATEARITKLSRFILLLVVAGALAFVFWGPTT
                     LVGLLIDASGPIMLQVLVVLIGALYWERATKEGAIISMIASEFFLILLWTKVIKMPIG
                     GIHNGVWAMILGVILFFVISLMTKPVDRKILDKFFSTFRE"
     gene            complement(3946..4203)
                     /locus_tag="VEL68_01830"
     CDS             complement(3946..4203)
                     /locus_tag="VEL68_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34738.1"
                     /translation="MSAGQKGFLVIVFIVLMIGISTPFQNVSAPIYLGFLPAPIFYLL
                     IVHILFVAFIGYMAFFTRLHGRAEDEEEFLAEVRREKEGGK"
BASE COUNT         1062 a         1215 c         1337 g          878 t
ORIGIN      
        1 gatcccgcga ttgtatgcag cgggagaagt gggttccttc ttcggtttcc tctatcaagg
       61 gggaaacaat tacccggagg cctgggcctt cggccaaatc gccgggagaa aggcggcggc
      121 ggagacgcct tggggcaggt agcacagcga acggtaggga tttgcctgcc gtccaggaag
      181 agtttagctt cggggccatc aagaccttga ccccccccat catccgctat atcatcacaa
      241 gaagcttaca ttccatcagg aggtgcatta tgagcgggaa gaaggctcta tggattctag
      301 taatattctg cagcgcaggt ctcgcggttc cgggggaaca ttttgcggct gaaaagaaat
      361 tccccaccaa gccgatcaac gtcatcatcc cctttcaacc cggggacacc gacaataacc
      421 tgcgcccctt taccgacaag atggtccaat acctggggca gccggtgaat tttgtatata
      481 aaccgggggc gtcgggagcg gtgggagcgg gatttgtggc caccgccgaa ccggacgggt
      541 acaccctggt gggttcccaa cagtccagcc tcgtcctggt gcccctcacc cagaaagggt
      601 tgacctattc gctgaagaat ttttctccgg tctgcggcct ggcggggggg tacaacatca
      661 ttggcgtgca gacaggagcc cgctggaaaa atattcagga gttgattgcc gaggccaagc
      721 agaatcccgg aaagatttcc tacgtttccg ggtccggggc tttgggtatc accacactga
      781 ttgccgaggc tttcttcaag gaagcggggg tcaagctgaa tttaatcccg gctcagggta
      841 gcgggccagc ggtgacggcc atcctcggag ggcacaccga tgcggtttcc tcccagatca
      901 ccccggcttt cccccatatc caggccggta cactccgagc cctggtggtg tccacggaga
      961 agcgggttcc cgcgcttccc gacacaccca ctgccgtgga gctgggctac aaagtaagcg
     1021 taccctccct ttatggcctg ctggccccca agggaactcc gaaggaagtg gtggataaga
     1081 ttgctttggc cgcaaaaaag gcggcggagg aacatcgcgg ggccattgag gcctcgctga
     1141 gcaaagccgg catgcaaatc ctttacgcgg ggcccgaggg gtttgctcaa tttctgaagt
     1201 cccaggatga ttactggacc caaacagtaa agaccttgga ccttaaaatg tagccagagg
     1261 cgttttggac cttgtcgtgg atggatggaa cttcgccccg gcttcaagag gttccattag
     1321 ggcttacggc gtgaccggcc aaaatcatta aaggaaaaga ggggaatgcc agagaaaact
     1381 attcaggtga aggatgtatt ggcggcgttg cagcagatta ccggtggccg ggtcatcatc
     1441 acggcggaaa acgcttttgc cgcagggagc ccgtttgtcg tccttaaaac ttcccatatt
     1501 cccggaaaag cggtgatgga gactccaggc ctggtctgcg gagatccgca aaaggaagtc
     1561 cgcagactgg ctgtatccat gactctcacg gagagccaga tcgaactggc cggggggacg
     1621 ggagtggacg ctatcgtggc ccaccacccg gtcgcagatg ccgccagctg cggcggcgtt
     1681 cctctccggg gctatctgaa cctctacaac ctggcggtct tcgaggtcca tgaggctttc
     1741 cacgggcttc acccgggtat cgctttgatt catggacata agcctttccg ggtggagatt
     1801 gcctacggag gtctgagcgg caacgtcttg ttcgtgggcc ggccccttcc ggagatcaag
     1861 aaggccgggg atatcttgaa ccggctggag ttattctccg acctggccgg ggaaaaaggc
     1921 tttctccgtc tcgagcagga ccagcgggaa tgcccggagc tgatggagac cagcatcgcc
     1981 gtggcccccg aggtcttaaa cggctccgaa aatgacccgg tgggaacggt gctccacttc
     2041 ttcccccaca cgggttttac ccccgatcat ctgcggtcgg cgaaacgaga acaccccgag
     2101 attaccaccg cgatcgtttc catcagccgg gtgaagaaag accatcccct ggtgaatacc
     2161 gcacgtgcct tggggttgac catgctggcg ggaaattccc acgccctgga aatcctggaa
     2221 aacggaatcc ccctggccta tgctttgaag accctgctcc cgggggtgga agtcgttctc
     2281 ttccgcgaga gagtgacttc ggttcccctg gagaaaatcg gatcaaaggt gatgagagat
     2341 tttgcggggg agatggcggc ccatcacctt ctaaagaggt gaggggtgaa ggcttgagag
     2401 ttgagaagtg agaattggga gatgcaacgg cccggttttt acaactctca cctcccaact
     2461 ggcaattctc aattcctcat tccctaaagg tgctgaagaa cttgtcaagg attttccggt
     2521 ccaccggctt ggtcatgagg ctgatcacga aaaagagaat cactcctagg atcatggccc
     2581 agacgccatt gtgaatcccg ccgatgggca ttttgatgac cttcgtccat aacaggatga
     2641 ggaaaaactc cgaagcgatc atggagatga tcgctccttc cttagtggcc cgttcccaat
     2701 aaagcgcccc gatcaggacg accaggactt gcagcatgat gggaccagag gcatcgatga
     2761 gcaggcccac cagcgtagtc ggcccccaaa atacgaaggc cagggctcca gcgacgacca
     2821 ggagaagaat gaagcggctc agcttggtga tgcgggcttc cgtggccgcc gcgggatcga
     2881 agaaacggac gtaaaaatcc cgcacgatga tggaggtgag gctgaggatt tggccggaga
     2941 ccgtggacat gatggccgcg agaatcgcca gaacgatgat gattccgaag accgggggaa
     3001 ccagttcgtt cagcatcagg gggaagacca ggtcgggatt cttgaggtcg gggtattgaa
     3061 cccggccggc gaggccgata aaaatcggcg gcagattgac aaacaaggca gccaggggaa
     3121 agatgaaggc cagcatcttg atggttcgca gccgcttggt ggcgtacatc cgctgagaga
     3181 tctgcggcca ggcaaagccc ccaagcccta ccaccatggc cgtggtgagg accatggagg
     3241 gggtccaaac tccagccggc cccgggaagg aaaccagctt cggactgata gccgaagcct
     3301 tctcgatcac cccgcctaat cccccggcta ctttgtcggc gatgaggaac atggccccca
     3361 gcatgcccac gagcatgatg atagattgca ccacgtccgt ccagacaacc gaccggaacc
     3421 cgcccagcag cacgtaaaac agaacgacta gggtgatata gaggagaccc atgttgaaag
     3481 agattacatt gttggtgact ccctggaggg tcaacccggc gcccttgatc tgcagcccga
     3541 tatagggaag cagggacccg atgctgatga tcatcgcgat cacacacacg aggttgctgc
     3601 cgtacctttc ggcaaagagg tcggtcatat tgatgaaccc gtgcttcttg cccaagaccc
     3661 acatgcggct gcccaggagg tacatgcaca aagagttgag caggatgttc cccgcaaccc
     3721 caaaaaagga aatccctgta cggtaatagg ccccgggcag cccgatgtag gaataggcgc
     3781 tgaacagggt ggaccagacg gtgcagagca ccacgaagag ccccagggtg cggcctccca
     3841 ggaagaaatc ctccatgctg ggacgggact ttccgtaccc ccagaagccg atggcgaaca
     3901 tcaggatggt ataaaggccg acccccacga tcaccagagt tggactcatt tcccgccctc
     3961 cttttccctg cgcacctcag cgagaaactc ttcttcgtcc tccgcacggc cgtggagccg
     4021 agtgaagaag gccatgtacc cgatgaaggc gacgaagagg atatggacga tcaaaagata
     4081 aaagatgggg gcgggcagga accccaggta aatgggggcg gagacattct ggaatggggt
     4141 ggatattccg atcatcagaa cgatgaagac aataaccaaa aaaccctttt gaccggcgga
     4201 catggatttc ctcctccgtt gcctcttttt ataatggaac tcgggggaaa tggtaagttg
     4261 cggcacagga aaagtcaagg gtattttggg tcttgcccac ccccagttct taaaaaagag
     4321 tccttcgaaa ataagatctc tggaaaggag ggggatctca tggggttcag ggcggagaaa
     4381 tttggatcat catcctgagg cggtggttct tccattggat cgggcctgtc ctggggggga
     4441 ttgaaacgga aaaatgcttt ggctctcttt tctgcttgga ggaatctcct ct
//
LOCUS       DASPRR010000050        12662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_16820, whole genome shotgun sequence.
ACCESSION   DASPRR010000050 DASPRR010000000
VERSION     DASPRR010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16820"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VEL68_01835"
     CDS             complement(<1..198)
                     /locus_tag="VEL68_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ34739.1"
                     /translation="MIQVRFAGLGGQGIILMGEVLGEAAVLDKKYVGQASSYGSEARG
                     SATKADVVISDSWIDYPEVTEA"
     gene            complement(371..1129)
                     /locus_tag="VEL68_01840"
     CDS             complement(371..1129)
                     /locus_tag="VEL68_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ34740.1"
                     /translation="MNQIIYEMINDQAFPTPFCPGCGHGILMNAVLRAFAELEWDLKN
                     TLFVSGIGCAAWIPSPHFKADTLHTLHGRAIAYATGAKLFNPGLKIMVISGDGDLSSI
                     GGNHLIHAARRNMDMTIVCANNSIYGMTGGQVASTTPLGSLTTTTPKGNLERPFDLCR
                     LAEAAGATFVARHPVATPIPLTRTLKKAITHKGFSFVEVLSPCPTQYGRRNAFRSAFE
                     MIQGLKSTCVSVEKAKGMSDEELAGKVVVGEFVS"
     gene            complement(1126..2268)
                     /locus_tag="VEL68_01845"
     CDS             complement(1126..2268)
                     /locus_tag="VEL68_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011973197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ34741.1"
                     /translation="MQYKKVKGPLAPGWYFMQGDEACAEGALAAGCRFYAGYPITPAS
                     EIMIRMVHRLPELGGAFIQMEDEIGSISAVIGAAWAGAKAMTATSGPGLSLMIEALGY
                     AVITETPCLIVDVQRAGPSTGQATRPAQGDMMMVKWGAHGDNEIVALSPWSVQEMYDL
                     TVKAFNFSERFRVPTFLLADEAVGHLRETLVIQEEVEVFDRVKIPGKPPFGTEEVNGV
                     PPMPAFGEGANLLVTGSTHDPWGFRKTQDSVVQARLTERLIRKIRDFKSEVIETDSHL
                     LEDAEIGVVAYGFTARAALAAVKTLRKQGVKAGLLRLVSIWPFADEAVAALGKRCRKI
                     FVPEMNRGQIAGELAKFTPTPVIPLPKTNGEVIEPAEIVEGIRRNQ"
     gene            complement(2278..2517)
                     /locus_tag="VEL68_01850"
     CDS             complement(2278..2517)
                     /locus_tag="VEL68_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ34742.1"
                     /translation="MKESVGKKKKTAAIHLNKKWCKGCYICLEVCPKKVFEKSPEVSE
                     KGFQPVIVAHPELCTSCLQCEMLCPDLAINVEKGE"
     gene            3042..3914
                     /gene="sucD"
                     /locus_tag="VEL68_01855"
     CDS             3042..3914
                     /gene="sucD"
                     /locus_tag="VEL68_01855"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXZ34743.1"
                     /translation="MSILVDENTRVICQGITGNQGMFHTRQAVAYGTKMVGGVTPGKG
                     GTTMDGLPVFNTVAEAVRETGANASMIFVPAPYAADAIMEAADAGIELIACITEGIPV
                     LDMVVAKGYLEGRKCRLLGPNCPGVITPGKCKIGIMPGYIHRPGPIGVTSRSGTLSYE
                     VVDQLTKLGLGQSTCVGIGGDPIIGMTFVEVLELFNRDPQTEAVMLIGEIGGTVEEEA
                     AGYIRQNFKKPVVAFIAGQNAPPGRRMGHAGAVISAGAGSPQRKMEALLAAGAKVVAN
                     PADMGPTMKQVLQG"
     gene            3931..4212
                     /locus_tag="VEL68_01860"
     CDS             3931..4212
                     /locus_tag="VEL68_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ34744.1"
                     /translation="MAKKEKHHIEVYKSWCKKCGICVAFCPAGVLAQDDTGAPYAKEP
                     DKCTGCQLCALRCPDFAINVTSPRKKEPKVSAKAPGESKEGATEQGEKA"
     gene            4229..5341
                     /locus_tag="VEL68_01865"
     CDS             4229..5341
                     /locus_tag="VEL68_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ34745.1"
                     /translation="MQGNEACAEGAITAGCRFFAGYPITPASEIAEQLSVRLPQVDGT
                     FIQMEDEIASMGAIIGASLAGAKSMTATSGPGFSLMQENLGYACIAEVPCVVVNVMRG
                     GPSTGLPTNPAQGDVMQARWGTHGDHPIIVLSASSVLECFTMTVKAFNFSERYRIPVI
                     LLLDEVVAHMREKVVLPKPSEVEVIDRVKPSVPPEWYLPYEDTSFGVPPMGIFGEGYR
                     YHVTGLTHDIRGFPTLRQDEIEPFLARLFRKISTHSDDIRLVEHFMTVDAEIVVIAYG
                     CVARSAHRAVLEARSQGMKVGMLKLITLWPFPRSFVELLIKAGKTLLVPEMNMGQVSR
                     EVKRVNEGMTQVLTLNKIDGTIISPKEILAKLKEKR"
     gene            5338..6231
                     /locus_tag="VEL68_01870"
     CDS             5338..6231
                     /locus_tag="VEL68_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXZ34746.1"
                     /translation="MIEDRPLIYKYLRHDKKFPHVWCPGCGIGTMLGSLIRAIDHIGY
                     TKDEIVLVSGIGCSGRLPVYVDFNTLHTTHGRALTFATGVKLAKPELKVIVIMGDGDA
                     TAIGGNHFIHAARRNIDLTAIIVNNSIYGMTGGQYSPTTPYGDRATTASYGTVEQSFN
                     ISQLAVCAGAVYVARGTVYHTRQLDQLIEKALQKKGFSVVEVISQCQTHFGRLNKRGN
                     QIEMLKWQRDHAVSVEKAKTLSAAEMEDKFETGVLVDREAPTYHEEYLKLRQKAKEAM
                     IPSMEQEEKEESDRREASDRH"
     gene            6337..6942
                     /locus_tag="VEL68_01875"
     CDS             6337..6942
                     /locus_tag="VEL68_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ34747.1"
                     /translation="MLDRYEIRLSGSGGQGLVLAGVILAEATGIYGGKHVVQTVSYGP
                     AARGGTSRADVVVSDKDIDYPKAMGLDILLAMNQMAYDESASELKPQGLLVVDSHLVT
                     QVTYPRVVKIPFTKIAKEKCGREQMANIVALGALSRLIPVTPQGGGVVSLEAMEAAVM
                     ARIPKGTEKQNKLAFDEGVKAAEEIQKSLVFEEPSEDSDQI"
     gene            complement(6998..7735)
                     /locus_tag="VEL68_01880"
     CDS             complement(6998..7735)
                     /locus_tag="VEL68_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXZ34748.1"
                     /translation="MKIFISHIQEDFQVALVLKKWIESAFADHCEVLTSTDPEGIPSA
                     AQSLEKDERAFEEIKALILLCSPDSIQKPWTSFEAGCAWLRKILIIPVCFSGLTAGQL
                     PQPLAIFPGFDLDQKDFGQGLFMTLAKELGISQLPAIQYRQMRQEVQEVLDAIPPGVS
                     SSSKEAGDVPLELIHLQILGVLKDNYGFTSAVLAQHFNLEEQKILPLLKRLIDGNYVY
                     ASPAGMGHVRYNLAKKGKDYLQESGLR"
     gene            8021..8479
                     /locus_tag="VEL68_01885"
     CDS             8021..8479
                     /locus_tag="VEL68_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXZ34749.1"
                     /translation="MSAEKKLKELGISLPQPPQPVANYVRAVKTGNLLFVSGHGPYQD
                     GKIKISGKVGKELTIEEGYQVARNVGLNCLASVKATLGDLDKVKRVVKLLGMVNCAED
                     FKDQPKVINGCSDLLVEVFGDAGKHARSAVGMQALPNGIPVEIEMILEVA"
     gene            8567..8986
                     /locus_tag="VEL68_01890"
     CDS             8567..8986
                     /locus_tag="VEL68_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXZ34750.1"
                     /translation="MIKKKFEDIQIGEKVSFGKTITEADVFAFAGITADFNPIHVNVE
                     FAKNSFFGKRVAHGMLTASLINQTLTNLGGLGTIHLTQTVKFMAPVFIGDTVTVVSEV
                     VSKDPGKNRMTVKSMVTNQEGKTVLEGEALIMMPKEK"
     gene            8997..9272
                     /locus_tag="VEL68_01895"
     CDS             8997..9272
                     /locus_tag="VEL68_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HXZ34751.1"
                     /translation="MAKYKVVARAKDSRCSLVNVGDPIVIENNMLNLKESKNVCVVAL
                     SAIQYSLFMMSKAKDPKDFGRDQVYTLQCPDPDTRVIYEISRKVMDE"
     gene            9347..9721
                     /locus_tag="VEL68_01900"
     CDS             9347..9721
                     /locus_tag="VEL68_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34752.1"
                     /translation="MLLDLEEMMRRHKQGEDPFELAIEKWVRIRDYLKKKADPERYRE
                     AFHCGSTKIIFCLDYKDHCPFCPMENVCFDSQSLYYQIMRSLRVYSLAGALLPREPVI
                     QLIESYIGDLRGYRDEWLKKSH"
     gene            complement(9810..10856)
                     /locus_tag="VEL68_01905"
     CDS             complement(9810..10856)
                     /locus_tag="VEL68_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34753.1"
                     /translation="MASTAKDILNNWETTLMEWNKPISQMNLNIEGTGLEKLIARLCR
                     EMDRKGIRFKPRFYLTDTWGCPNKVPIIGIPFYYANPSLSQIEDRMNGDLEDEHELMM
                     TLRHEAGHAINYAYRLYEAPEWQNIFGRFDEPYRDFFRPNPQSREFVKHLYQQVGSYA
                     GRIYAQKHPDEDFAETFAVWLTPRSNWRAKYRNWSAFKKLKYVDRLIKEIGPKKPLVT
                     DGERINPIGKVNLTLLEYYNKSEERYRQRAQGYVDDVLMEIFSTNGKGEKRIPAAGFI
                     DKNRHHLVERISHWTGEKTSSVDALIDKLSDRAKELGLNLSPRRQSRKLIEVTALATT
                     LVMNYTYEGKFTLN"
     gene            complement(11047..11598)
                     /locus_tag="VEL68_01910"
     CDS             complement(11047..11598)
                     /locus_tag="VEL68_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HXZ34754.1"
                     /translation="MAHKARDAVFISLFAALTAVGAFIKIPIPHVPFTLQTLMVMFAG
                     LLLGSRRGALSQVLYLILGLMGFPIFAQGGGPAYILQPSFGFLIGFIAGAFAIGKVLD
                     RSTSLTLSRTLFALVLGQGIIYLFGLSYLYFNLNFILQKPISLSSVFVIGFLIFIPGD
                     VVKTIIAALLMAPIRQRLFPLNR"
     gene            11810..12067
                     /locus_tag="VEL68_01915"
     CDS             11810..12067
                     /locus_tag="VEL68_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34755.1"
                     /translation="MAGELKSAWELAMEKAKKMGKDEIPSLSPDQKEEIAQVRKVYEA
                     KFAEVEILVQDKEKKDLEIDRLRRERDRKIESVYEKARGKK"
     gene            12191..>12662
                     /locus_tag="VEL68_01920"
     CDS             12191..>12662
                     /locus_tag="VEL68_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXZ34756.1"
                     /translation="MPRMTGGEAVVEALKREGVEYLFSIPGVQIMSVYDALYGEKDLR
                     LVVVRHEQTALYMADGYARVQGRPGVGLVVPGPGVQNTLAALGTAYACSSPVLLVAGQ
                     VETKDLGRDGGALHEVNDQLDMVRPVTKWCKRVMRVEEIPDAIREAMRQMATGRP"
BASE COUNT         2940 a         3338 c         3688 g         2696 t
ORIGIN      
        1 agcctccgtg acctccgggt aatcaatcca ggaatcggaa atcaccacgt ccgccttcgt
       61 ggccgagccc cgggcctcgg acccgtagga agaagcctgg ccgacgtact tcttatccaa
      121 aaccgcagcc tcccccagga cctcccccat gaggatgatt ccctgacctc ccaacccggc
      181 aaaacgaact tgaatcatga taacctcttt tttatgggac gcagatgaac ataaatacaa
      241 tccctggagt tagaagtgcc ttaagtgaaa agaccgcaaa acaatatggg acacagatga
      301 acacagaaaa aaattttaac ttgaggccct taaggctcta ggcactctga actctaggcg
      361 ctctaggcac tcagcttaca aactccccca ccaccacttt cccggctaat tcctcgtcgc
      421 tcatcccttt ggccttttcc acggagacgc aggtgctctt cagcccctgg atcatttcaa
      481 acgccgagcg gaaggcgttc cttcttccgt attgggtagg acagggggag agaacttcga
      541 cgaaggaaaa ccccttgtgg gtgatggctt ttttcagcgt gcgggtcagg gggatggggg
      601 tggccacggg gtgccgggcc acgaaggtag cccccgctgc ctccgccagc ctgcaaaggt
      661 cgaaaggacg ttctaagttt ccctttggag tcgtggtggt cagggagccc aggggggtgg
      721 tggaagccac ctgccccccg gtcattccat aaatggagtt attagcgcat acgatggtca
      781 tgtccatatt tctccgggcg gcgtggatca ggtggtttcc tccgatggag ctcaggtccc
      841 cgtctccgct gatcaccatg atcttcagtc cgggattgaa aagcttggct ccggtggcat
      901 aggcgatggc ccgaccgtgc agggtgtgca gggtgtcggc cttgaaatgg gggctgggaa
      961 tccaggcggc gcaaccgatt ccggaaacga ataaagtgtt cttcaggtcc cattccagct
     1021 cggcaaaagc ccggagaacg gcgttcatca ggatcccgtg cccgcacccc gggcaaaaag
     1081 gagtgggaaa ggcctgatcg ttaatcatct cgtaaattat ttgattcatt ggttcctccg
     1141 gattccttcc acgatctcgg ccggttcgat gacctcccca ttggttttgg gaagggggat
     1201 gaccggagta ggggtgaatt tggccagttc ccccgcgatc tgaccccggt tcatctccgg
     1261 gacgaaaatc ttccggcagc gcttgcccag cgcggcgacg gcctcatcag caaaaggcca
     1321 gatggagacg agacggagca atccggcttt tacgccctgt ttccggagcg tcttcactgc
     1381 cgcaagggct gcccgggcag tgaatccata ggccacgacg ccgatctccg catcctccag
     1441 cagatgggaa tcggtctcga taacttccga cttgaagtcc cggatttttc ggatcaaacg
     1501 ctccgtcagc cgggcctgaa cgaccgagtc ctgggttttg cgaaaccccc aggggtcatg
     1561 ggtggacccg gtaaccagaa ggttggcccc ttcgccgaaa gcgggcatgg gaggaacgcc
     1621 gttgacctcc tcggttccga aggggggctt tccgggtatc ttgacccggt cgaagacttc
     1681 cacctcttct tggatgacca gagtctcccg cagatggccc acggcttcat ccgccaggag
     1741 gaaggtggga acccggaaac gctcggagaa gttgaaggcc ttcaccgtca ggtcgtacat
     1801 ctcctggacc gaccaggggg agagggcgac gatctcgttg tctccatggg cgccccattt
     1861 taccatcatc atgtctccct gggccgggcg ggtggcctgg ccggtgctgg gaccggcacg
     1921 ctgcacatcg acaatcaggc agggggtttc ggtgatgacg gcgtagccga gggcctcgat
     1981 catcaggctg agacccggac cggatgtggc agtcatggct ttggccccgg cccaggcggc
     2041 gccgatgacc gcggagatgg atccgatttc gtcctccatt tggataaaag ccccccccaa
     2101 ttcgggaagg cggtggacca tgcggatcat aatttcactg gccggggtta tcgggtagcc
     2161 agcataaaaa cggcagccgg cggcaagagc cccttcagcg caggcctcat ccccctgcat
     2221 gaaataccaa ccgggggcta acggcccctt gaccttcttg tattgcatgg gtctatccta
     2281 ttctcccttt tctacgttga tggccaggtc cggacagagc atttcgcact gcagacagct
     2341 ggtacagagt tccggatggg cgacgatgac gggctggaag cctttttccg acacctccgg
     2401 ggatttttcg aatactttct tggggcagac ttccaggcag atatagcagc ccttgcacca
     2461 tttcttgttg aggtggatgg ctgcagtttt cttctttttt cccacgcttt ctttcaattc
     2521 agggctctcc ttcccttcag cggagccgaa aaatcacccg cctaaatcta gccaaatata
     2581 tttgaaattt caatccggtt ctctcgccct tttagatggg agagggcggg gctgggggtg
     2641 gaacgatcat tcatcccctc ccctttattc cctcccgccc agggagggga taattttaaa
     2701 aatctgagaa aaaatttctc agaatgaaag gtgtgagaac ctaaaattaa tatggcttag
     2761 gaaacgagga ggcgtcaagt ttttttcttt cagtcttggg cctcagggtg attctttcca
     2821 tgaccgcggt catggggatt ctggcgggag aggatagaat agtttataga aggggaatgg
     2881 ttgccccttc ggttttctct cccgggaaaa tcctaaatta tttgaatgaa ataggctgcg
     2941 ggaggtccgg gttgtccggg ttttttgctt gacggaagga gcgcttattc attaggatac
     3001 agggaagatt tccattccaa ccatgacctg cggaggaaag gatgagcatc ctggtggatg
     3061 agaatacccg ggtgatctgt cagggaatta ccggcaatca gggaatgttc catacccgcc
     3121 aggccgtggc ctacgggacc aaaatggtcg gaggggtgac cccggggaag ggcggaacca
     3181 ccatggacgg tcttcccgtt ttcaacacgg tggccgaagc cgtgcgggag accggggcca
     3241 atgcctccat gattttcgtc cccgccccct atgcggccga tgcgatcatg gaagcggctg
     3301 acgccgggat cgagctcatc gcctgcatca ccgagggaat cccggtcctg gatatggtgg
     3361 tcgccaaggg gtacctggag gggcggaagt gccgcctcct gggccccaac tgtccggggg
     3421 tcatcacccc gggaaagtgc aagatcggca tcatgcccgg ttacatccac cgtcccgggc
     3481 cgatcggagt cacttcccgc agcggtaccc tcagctacga agtggtcgat caactgacta
     3541 aattgggatt ggggcagtcc acctgcgtgg gcatcggcgg tgaccccatc atcggaatga
     3601 ctttcgtgga ggtccttgaa ctcttcaacc gcgatcccca gaccgaagcg gtcatgctga
     3661 tcggagagat cggcgggaca gtggaagaag aggcggctgg ctacattcgc caaaatttca
     3721 agaagccggt agtggccttc attgccgggc agaacgcgcc tccgggccgg cggatgggac
     3781 acgccggggc ggtgatttcc gcgggggcgg gttccccgca gcgcaagatg gaagccctgc
     3841 tcgccgccgg ggccaaggtt gtggccaacc cggcggacat gggcccgacc atgaagcaag
     3901 ttttacaggg atgaaaggga accgaagttc atggccaaga aagaaaagca tcatatcgag
     3961 gtttacaaga gctggtgcaa gaagtgcggc atttgcgtgg ccttctgccc ggccggagtc
     4021 ctggcgcagg atgataccgg cgccccctat gccaaggagc ccgacaagtg cacggggtgc
     4081 cagctgtgcg cgctgcgttg cccggacttc gccatcaacg taacctctcc caggaagaaa
     4141 gaacccaagg tctcggccaa agccccgggc gaaagcaagg aaggagcgac agaacagggt
     4201 gaaaaagcct gatcccagag ccaagctcat gcagggcaac gaggcctgcg ccgaaggggc
     4261 catcactgcc gggtgccggt tctttgccgg ctatcccata actcccgctt cggaaatcgc
     4321 cgagcagctt tcggtccgcc taccccaggt ggacgggacc ttcatccaga tggaagatga
     4381 gatcgccagc atgggagcca tcattggggc ctccctggcc ggggccaaat ccatgactgc
     4441 cacgtctggg ccggggttct cccttatgca ggaaaacctc ggctatgcct gcatcgccga
     4501 agtaccctgc gtggtggtga acgtaatgcg gggcgggccg agcacagggc tgcccaccaa
     4561 tcccgcccag ggggacgtga tgcaggcgcg gtggggaacc cacggggacc atcccataat
     4621 cgtcctgagc gctagctcgg tcttggaatg cttcaccatg acagtgaaag ccttcaattt
     4681 ttcggagagg taccggattc cggtaattct ccttttggat gaagtggtgg cccacatgcg
     4741 tgagaaggtg gtcctgccca agccgtcgga ggtggaagtg atcgaccggg tcaagccttc
     4801 cgttcccccc gagtggtacc ttccctacga ggataccagc ttcggagtac cccccatggg
     4861 aatcttcggg gaggggtacc ggtaccacgt gaccggcctg acccacgaca ttcgcggatt
     4921 ccctaccctg cgtcaggatg agatcgagcc ctttttggcc cgcctcttcc gtaagatttc
     4981 cacccactcc gacgatatcc ggctggtgga acacttcatg accgtggacg ccgaaatcgt
     5041 cgtcatcgcc tacggctgtg tggcccgctc cgcccaccgg gcggtcctgg aggcgcggag
     5101 ccaggggatg aaggtgggga tgctgaaatt gatcaccctc tggcccttcc cccgttcctt
     5161 cgtcgaactg ttgatcaagg cgggcaagac cctcctcgtt ccggagatga acatgggcca
     5221 ggtctcccgc gaggtgaagc gggtgaacga gggaatgacc caggtgctca ccctgaacaa
     5281 gatcgacgga acaatcatca gccccaaaga gattctggcc aagttgaagg agaagagatg
     5341 atcgaggatc gccccctaat ttacaagtat ctgcgccatg acaagaagtt tccccatgtg
     5401 tggtgccccg gctgcggcat tggtaccatg ctgggttcac tcatccgggc catcgatcac
     5461 atcggctata cgaaggacga gatcgtactg gtctccggca tcggctgctc ggggagattg
     5521 ccggtgtacg tggatttcaa caccctccac acgactcatg gaagggctct gaccttcgcc
     5581 accggggtaa agctcgccaa gcccgagctg aaagtcatcg tgatcatggg cgatggggac
     5641 gccacggcca tcggggggaa ccatttcatc cacgccgccc ggcggaacat cgacctcacg
     5701 gccatcatcg taaacaacag catctatggc atgaccggcg gccagtattc tcccaccacc
     5761 ccctatggag accgggccac caccgcatcc tacgggactg tggaacaaag tttcaacatc
     5821 tcccagctgg cggtctgcgc cggggcagta tacgtcgccc gggggacggt gtaccatacc
     5881 cggcagctcg accagctgat cgagaaggcg ctccagaaaa agggattcag cgtggtcgag
     5941 gtcatcagcc agtgccagac ccatttcggc aggctgaaca agcggggcaa tcagatcgag
     6001 atgctcaagt ggcagcggga ccatgccgtg tcggtggaga aagccaagac cctgtccgcc
     6061 gcggagatgg aagacaagtt tgagaccggt gttctggtcg accgggaggc cccgacgtac
     6121 catgaggaat acctcaagct ccgccagaaa gccaaggaag ccatgatccc gtcgatggag
     6181 caggaagaaa aagaggaaag cgaccggcgg gaagcatccg atagacatta aatgcagttc
     6241 ggagttcgga gtggggagtt cagagttaaa tccaagaacg aacttgcggc taattactct
     6301 ggggactccg aactttaggc actacaggga attcttatgc tcgatcgtta tgaaattcgt
     6361 ctaagcggtt caggaggcca gggcctggtt ttggccggag ttatcctggc ggaggctacg
     6421 ggcatctacg gtggcaaaca cgtggtccag acagtcagct acgggccggc agcccggggg
     6481 gggacgagcc gggccgacgt ggtggtgagc gataaggata tcgattatcc caaggccatg
     6541 ggcctggaca tcctcctggc catgaatcag atggcctacg acgaatccgc ctccgagctg
     6601 aagccgcagg gcctcctggt ggtggattcg cacctggtca cccaggtgac ctatccccgc
     6661 gtggtgaaga tccccttcac caagatagcc aaggaaaagt gcgggcggga gcagatggcc
     6721 aacatcgtgg cgctcggagc cctgagccgg ctcattcccg tcacccctca gggcggcggg
     6781 gtcgtttccc tcgaggccat ggaggcggcg gtcatggccc gcattcccaa ggggaccgag
     6841 aagcagaata aactagcctt cgacgaaggg gtcaaagctg ccgaagaaat ccagaaaagc
     6901 ctggtcttcg aagaaccttc tgaagacagc gaccagatct aaggacttcc cgtcctcccc
     6961 ctccgactct tcctcctgga ataacccccc aaaaaattca ccgaagcccg ctttcctgga
     7021 ggtagtcctt tcctttcttg gccaggttgt accggacatg gcccatgccc gctgggctgg
     7081 catagacata attcccgtcg atcaaccgct tcaggagggg cagaatcttc tgctcctcca
     7141 aattgaaatg ttgggccaat accgctgagg tgaatccata gttgtccttc aagaccccca
     7201 ggatctggag gtgaattaac tccaggggga cgtccccggc ttcttttgaa cttgaggaga
     7261 cgcccggggg aatggcatcc aagacctcct ggacctcctg cctcatttgc cggtactgaa
     7321 tggccggcag ctgggagatt ccgagttcct tggccagggt cataaagagc ccctggccga
     7381 aatccttctg atccaaatcg aatccgggga aaatcgccaa tggctggggg agctggcctg
     7441 cggtgagccc tgaaaagcat accgggatga taaggatttt cctgagccaa gcgcatccgg
     7501 cctcaaaaga agtccacggc ttctggatag aatcggggct gcagaggaga atcagggctt
     7561 tgatttcttc aaaggcccgt tcatcctttt ccagggattg ggccgcggag gggataccct
     7621 cggggtcggt actggtcagg acttcacaat ggtcggcgaa agccgattcg atccacttct
     7681 tcaataccag ggcaacctgg aagtcttcct ggatgtggct gatgaatatt ttcatctgga
     7741 tttctttccc tgaaattcgg gcttttcggt tgattataaa gaaaatgaat cccactttca
     7801 acaggcggtt taggtccggc ccagctcgca ggtctaattt cttgacaata tggggtaaga
     7861 gggttatttt tgaatcaggg tcaaaggcaa atcgaaacat taagagggta atcggttttt
     7921 ttctatgctc caggatttcg tgcttcctgt tgagatgggt cctattcgac ctttagccgg
     7981 ttcattttga aagaaatcat cgacgaacaa ggaggctccg atgagtgccg agaaaaagct
     8041 gaaagaatta ggaatctccc tcccgcagcc gccccagccg gtcgccaact atgtgagggc
     8101 ggtcaaaaca gggaacttgc tattcgtgtc cgggcatgga ccgtaccagg atggaaagat
     8161 caagatttcc ggcaaagtcg gaaaggaact gaccatcgag gaaggatacc aagtggctcg
     8221 caacgtaggg ttgaactgcc tggcttcagt caaagcaacc ctcggagacc tggataaggt
     8281 caaacgggtg gtcaagcttc tagggatggt gaactgcgcg gaggatttca aggatcaacc
     8341 caaagtaatc aacggctgct cggaccttct ggtcgaagtt tttggagatg caggaaagca
     8401 tgcgcgctcg gcggtgggga tgcaagccct tcccaacggg atccccgtgg agatcgaaat
     8461 gatcctggag gttgcttgaa tcagtagggc gggatccccg atcacgcctt tacgcggtgc
     8521 gttcggggaa cgcaccctac attctgacgc cctctaagga ggaaggatga tcaagaagaa
     8581 gttcgaagat attcagattg gggaaaaagt gagctttggc aagaccatta cggaagccga
     8641 tgtcttcgct ttcgccggga tcacggcgga tttcaacccc atccatgtga acgtagaatt
     8701 cgccaaaaat tctttttttg gaaagcgggt agctcacgga atgctcacgg ccagtctgat
     8761 caaccagacc ctgaccaatc taggagggct ggggaccatt cacctgactc aaaccgtgaa
     8821 gttcatggcc ccggtcttca ttggcgacac ggtcaccgtg gtatctgagg tcgtcagcaa
     8881 agacccgggg aaaaatcgga tgacggtcaa atccatggtt accaaccagg aaggaaagac
     8941 cgtcctggaa ggggaagccc tgatcatgat gcccaaagaa aaatagggag gaagagatgg
     9001 cgaagtacaa agtggtcgcc agagcgaaag acagccgatg ctccttagta aatgtggggg
     9061 acccaattgt gatcgaaaac aatatgctga acctgaagga gtcgaagaac gtttgtgtcg
     9121 tggccctcag tgcgatccaa tattccctct tcatgatgag caaggccaaa gaccccaagg
     9181 atttcggaag ggatcaggtc tataccttgc agtgtccgga cccggacacg cgagtgattt
     9241 acgagatatc ccggaaggta atggacgaat aaacagcgat ctgcagtcag ccttcggcag
     9301 gagaaaatag agctctaaag gttggggtga gttttcggaa gtggccatgc tgctcgatct
     9361 agaagagatg atgcgccggc acaagcaggg agaggacccc tttgaactgg ccattgaaaa
     9421 gtgggttcgc atccgggatt acttaaagaa aaaggcggac ccggagcggt atcgggaagc
     9481 ctttcactgc ggctcgacca agatcatctt ctgcttggac tataaggacc actgcccttt
     9541 ttgcccaatg gagaatgtct gctttgacag ccagagcctc tattatcaga tcatgcgttc
     9601 cctccgggtg tattccctgg cgggggcgct ccttccccga gagccggtca ttcagctcat
     9661 cgagtcctac attggcgatc tccgcggata ccgggatgaa tggctgaaga aaagccatta
     9721 agttggattg cgcattgagg aatgagtaaa aggaacgttt gggctcgccc atggaaaatt
     9781 gcgcaatgcg aaatgcgcat tacgcaattt cagttgaggg tgaattttcc ttcgtaggtg
     9841 taattcatca ccagtgtggt ggccagggct gtaacctcaa tcagcttccg ggactgacgg
     9901 cgggggctga ggttgaggcc gagttctttc gcccggtcgc tcagcttgtc gattaaggca
     9961 tccaccgaag aggttttttc gccagtccag tgagaaatcc tctccaccaa gtggtgacgg
    10021 tttttgtcga tgaatccagc agccgggatt cgcttctctc ccttgccgtt ggtgctgaaa
    10081 atttccatca atacatcgtc cacatatccc tgggcccttt gccggtacct ctcttcactt
    10141 ttattgtaat attccagaag agtgagattg actttcccga tgggattgat gcgttccccg
    10201 tcggtcacca ggggtttttt cgggccgatt tcctttatca agcggtccac gtacttaagc
    10261 ttcttgaaag cgctccagtt tcggtatttg gctctccagt ttgaacgggg agtcagccag
    10321 acagcaaagg tttcggcgaa atcctcatcg ggatgcttct gggcataaat gcggcctgca
    10381 taggacccaa cctgctggta gaggtgcttt acgaactccc gcgattgggg attgggccgg
    10441 aaaaaatccc tgtaaggctc atcgaaccgc ccaaaaatat tctgccactc gggtgcctca
    10501 tagaggcggt aggcataatt tatggcatgc ccagcttcat ggcgcagggt catcatgagc
    10561 tcgtgttcat cttccaggtc tccattcatc cggtcctcga tctgggagag ggatgggttg
    10621 gcgtagtaga aggggatccc aatgattggc actttgtttg gacaacccca ggtatctgtt
    10681 aggtaaaatc tcggcttaaa gcgaatcccc ttacggtcca tttctcgaca caaccgagcg
    10741 atcagctttt ccaggcccgt cccttcaatg ttcaggttca tttggctgat aggtttattc
    10801 cactccatca gcgttgtttc ccaattgttt aggatatctt tggctgtgct ggccatggtt
    10861 ccattccctc gaaagcgcat gagctttaaa ggttttgaat ttaataaggt ttttattttg
    10921 cagctggaga gatcgggaaa accctgggag gttctttctt tttcttatac ctaattctaa
    10981 aatagggtat aaatggcttt ttgtcaagaa aaagcaagat aaatataatt gaataaattc
    11041 ccaatgttat ctatttaaag ggaaaagacg ctggcggatg ggggccatca agagggctgc
    11101 gatgattgtc ttaaccacat ccccagggat gaaaatcagg aacccaatga caaagaccga
    11161 agagagagag atgggttttt gaaggatgaa attcagattg aagtaaagat atgaaaggcc
    11221 aaagaggtag atgatccctt gtcccagcac cagcgcaaaa agggttcggg atagggtaag
    11281 gctggtgctc ctatcgagaa ccttcccaat ggcaaaagct ccggcgatga acccaatgag
    11341 aaacccaaaa gaaggttgca ggatataagc aggccctcct ccttgggcga agatgggaaa
    11401 tcccatcagt cccaggatga gatagagaac ctggctcaaa gccccgcgcc ggctccccag
    11461 aagaagaccg gcaaacatga ccatcagagt ctgcaaggtg aaagggacat ggggaatggg
    11521 gatcttgata aaagccccca cggcggtaag agcggcaaat agggatatga agactgcatc
    11581 ccgagcttta tgggccattg gttcttccca aaccattctc tttttatctt attcaacttt
    11641 tttcggtcct gtcaaacctt tctactgccg ggccgcgggc cgttttccct ttgttccgta
    11701 tttctgacct gcgattcccg ttcggattct gttgatcccc caggggtttt tggtataaac
    11761 tctttccatc cttgcggcgt gttttgccaa caaaaaggag ggctgggcta tggcaggaga
    11821 attgaaaagc gcttgggaac tggccatgga gaaggccaaa aaaatgggta aggatgaaat
    11881 cccctcctta tcccccgacc agaaagaaga gatcgcccag gtccggaaag tgtacgaggc
    11941 caagttcgcg gaagtggaga ttctggtcca ggacaaggag aagaaagacc ttgaaattga
    12001 ccgccttaga agggagaggg accgaaagat cgagtcggtc tacgagaagg caaggggaaa
    12061 gaagtgaaag ggcaagggga agagaaagga ttgaccggaa aatggaagaa ttaaagtcac
    12121 cccgatccgg aagaggccgg agcataggag gcgttgaatt tgaccgtggg ttaggaacag
    12181 gagagattgt atgcccagaa tgaccggagg agaagcagtc gttgaagccc tgaaaaggga
    12241 aggggtggag tacctttttt ccatccccgg ggtgcagatc atgagcgtct atgacgccct
    12301 ttacggggag aaggaccttc gcctggttgt ggtccgccac gaacaaacag ctctctatat
    12361 ggctgatgga tatgcccggg tgcaggggag gcctggagtt ggattggtgg tcccgggtcc
    12421 tggggtccaa aatactctcg ccgcattggg aacggcctat gcctgctctt cgccggtcct
    12481 gctcgtggct ggacaggtcg agacgaaaga cctgggaagg gacggtgggg ctctccacga
    12541 ggtaaatgac caactggata tggtccgccc ggttaccaaa tggtgcaagc gggtcatgcg
    12601 ggtcgaagag ataccggacg cgattcgaga agccatgcgc cagatggcca cgggccggcc
    12661 gc
//
LOCUS       DASPRR010000051         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1464593, whole genome shotgun sequence.
ACCESSION   DASPRR010000051 DASPRR010000000
VERSION     DASPRR010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464593"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1736
                     /locus_tag="VEL68_01925"
     CDS             264..1736
                     /locus_tag="VEL68_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ34757.1"
                     /translation="MPTQERRVSVVIAGGGISGLSAGWKLSKAGFQDFELLELETEVG
                     GVSRGGGNSISSYPWGAHYVPLPTEESRAVRELFEELGVIEGRTGAGQPVYKEKYLCF
                     SPQERLYIHGKWQEGLLPVLGATRKDLDHFQKLKEIVLGFKRRRGKDGRKAFAIPMEK
                     SSRDSDLLALDRISFREFLNRQGLDSAPLHWYANYACRDDYGCHYAEVSAWAGLHYFC
                     SRDGGGDSDESTVLTWPEGNGWIVKQLQHKLRPKIKTHSLVYRLTDAGNEVRVDSYDP
                     IENSSTRIYAQQVIYACPRAFAPYLIEGNGLIDSSYLKEFQYSPWMVANLSLNSIPQE
                     DSGVPLAWDNVIYDSPSLGYVVATHQSLATHLSKTVLTYYHAMVQGSPVQERTHLLQM
                     SWNECAEFIIRDLSHAHPKIRDLISHLDIFRWGHAMVQPKVGFVWGEARKRAAHPHGN
                     IFFAHSDLSGFSIFEEAQYRGVLAAERILAKQKVPFSSSL"
BASE COUNT          468 a          531 c          582 g          461 t
ORIGIN      
        1 aaggacatgg ccccggtgga ggcggaggtg aaccgcctga acaaccaggt cctggtgcag
       61 tactacgagg ccgaatggca caaggtgacc gattagtgaa ccgccggagc tttcttcttt
      121 catccgcagg acttctttcc gcctttgccc tctcccttat cccagggagc cggagaaaag
      181 ggccaaaggg gctccctttc agcgggtcga tcgtcggccc atcctacaac ctgggccaca
      241 ggcttctgaa gggtgacttt ccaatgccta ctcaggagcg aagggtttca gtggtaattg
      301 cgggcggggg aatttcgggg ctttccgccg ggtggaagct gagcaaggcc ggattccagg
      361 attttgagtt gctggaactt gaaaccgagg tgggaggcgt ttcccgggga ggagggaatt
      421 ctatctcttc ttatccctgg ggagcacatt acgtgccgct tccgactgaa gagtcccggg
      481 cggtcaggga actctttgaa gaattggggg tgatcgaggg acggacaggg gcgggccaac
      541 cggtctacaa agagaagtac ctctgcttct ccccccagga acgactctat atccacggca
      601 aatggcagga aggacttctg ccggtcctgg gcgcgacccg aaaggacctg gaccattttc
      661 aaaagctcaa ggagatcgtc ctcggtttca aaaggcgccg cgggaaagac ggccggaaag
      721 cctttgccat ccccatggag aagagctccc gggattccga cctgctggcc ctcgaccgga
      781 tttcctttcg agaatttctg aaccggcaag ggctggattc ggctcctctc cactggtacg
      841 ccaattatgc ctgccgagac gactacggct gccattatgc cgaggtctcg gcctgggcgg
      901 gtcttcacta tttttgcagc cgtgatgggg gaggagattc ggatgaatca accgttctga
      961 cctggccgga aggaaacggc tggatcgtca agcaactcca gcataaactg agacccaaga
     1021 tcaagaccca ttctctcgtc tatcgcttaa cggatgcagg aaatgaagtc agggtggatt
     1081 cttatgatcc gatagaaaat tcttcaaccc gtatctacgc ccaacaagtc atctacgctt
     1141 gccccagggc cttcgccccc tacctgatcg agggaaatgg cctgatcgat tcctcctatt
     1201 taaaagaatt tcagtacagc ccctggatgg tggcgaattt gagcctgaat tccattcccc
     1261 aggaggattc cggcgtaccc ctggcctggg acaacgtcat ctatgacagc ccgtccctgg
     1321 gttatgtggt tgccacacac cagagtttgg ccacgcacct atcgaaaacc gtcctgactt
     1381 attatcatgc catggtccag gggtctccgg tgcaggaaag aacccatctt ttgcagatgt
     1441 cgtggaacga gtgtgcggaa ttcatcatcc gagacctctc ccatgcccat cccaaaatcc
     1501 gggacctgat ctctcacctg gacatctttc ggtggggcca tgcaatggtt cagccaaagg
     1561 tggggttcgt ctggggggag gctcgcaaac gggcggctca cccccacgga aatatatttt
     1621 ttgctcattc tgatctaagc ggcttttcca tttttgagga agcccagtat cggggagtat
     1681 tggccgccga aagaatcctg gccaaacaga aggttccctt ttcttcctcc ctgtgaagga
     1741 ggaagaggca tagagcataa caaaaaaaat tgcagaagag ggaagagttt ggtggtttga
     1801 aacgggtttc ccagaaatcc ctcctggcct cccttttcca aagggaggag aggttactca
     1861 cgggagttcg gtgaatcgtg aggccttttc cccccttgaa aaagggggat ggagggggat
     1921 ttgaatcctg cggtactgca gtcgatgttc tgaagaaaag aatttagaaa gcgcctacgc
     1981 tctgtctttg taaagaagaa ggtgacagtt tagactttcg agatttggtc gagaaggtct
     2041 gg
//
LOCUS       DASPRR010000052         4648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_498654, whole genome shotgun sequence.
ACCESSION   DASPRR010000052 DASPRR010000000
VERSION     DASPRR010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498654"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VEL68_01930"
     CDS             complement(<1..1539)
                     /locus_tag="VEL68_01930"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HXZ34758.1"
                     /translation="MPVSPIYVQGARQNNLKGITVHVPVGAVTIVTGVAGAGKSSLAF
                     DVLYAEGYRRYVETFSPYARQFLERLDRPEAERIDDILPAIAISRTAPVRTSRSTVGT
                     MTSIDDYLRPLFARASILHCKGCGRPVHRYSPSMIFDALLAASAGKNALICFTRRVGR
                     FSPAALRDVFRQAGFARVLEGGRPILVEEAELKPRDEAVTVVLDRLLVDPGRRRRIVD
                     SVESALRFGEGRMEAWIEGEREPLRFSEKLHCERCGLDYGTPSPAIFSFNNPIGACEA
                     CHGFGRVIDIDPDLVIPDAGMTIAEGCIKPFQTPAFFECQKDLLRFLKRKKIPGDRPW
                     RELDPKVQRLIWEGEGNGSWYGIHGLFKWLEGRRYKKQSRIFLARYRRYLPCPDCGGG
                     RLKPPALLYRLGDKTLPEVEKMSIAEAEEFFHHRTPPGGDRATDLLLQEIRGRLEFLQ
                     DVGLGYISLGRQSRTLSGGETQRVTLATALGASFTSTLYVLDDPSVGLHPRDKERLAV
                     VLRKL"
     gene            1759..2673
                     /locus_tag="VEL68_01935"
     CDS             1759..2673
                     /locus_tag="VEL68_01935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HXZ34759.1"
                     /translation="MNEKMAQTFNRYRDVLPDYEGFLSAVEQPIPVSIRANTLKIDPG
                     PFQAFMRERGYDLKPVEGIEEAFSLQGVESPGATLEYFWGYYQVQGLTSMLPAKVLSP
                     RPGEIILDLCAAPGGKTTHLAQLMGDRGLVVANDFKKERTHVLRSHIDRLGILSVLVC
                     CYSGQAFPLRRKFDRVLLDPPCSAEGTYRAGLAPTRSENPKVAQHLVRVQKGLLQQGL
                     AVLRPGGTLVYSTCTYAPEENEAVVNEAVKEGEAELLPISIPFSHSRGLPSWKEESYH
                     PDMVKTVRLYPHQVNSWGFFIAHLRKRS"
     gene            2648..3127
                     /locus_tag="VEL68_01940"
     CDS             2648..3127
                     /locus_tag="VEL68_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34760.1"
                     /translation="MPTFASEAERKEILDYFSNRFAIPEKAFEGFCFLLGKRKIWVVR
                     GHPELERLMKQLKFESAGIPLLRTKTSMWKPTTTGLHLFGLHATRNVIDLEGDALQAF
                     LKREDSDGSLPLEPGFVIVRCEGKVLGCGIYGKGKLRSQLPEKRGEPFRGKLYPQDF"
     gene            complement(3194..3505)
                     /locus_tag="VEL68_01945"
     CDS             complement(3194..3505)
                     /locus_tag="VEL68_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34761.1"
                     /translation="MAAEPRVLLTVILHHDQSKTLDEIMAHLKKTGFYRDFPPDGTEL
                     VSWVVAMSFGFILHVSVDPAKIRNLNRFMEQKSWGAFRYQVYPSYDFKAIAEQLKKDH
                     A"
     gene            complement(3597..4355)
                     /locus_tag="VEL68_01950"
     CDS             complement(3597..4355)
                     /locus_tag="VEL68_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXZ34762.1"
                     /translation="MAAEGPGRVKEQSKSDGTAFFVQWTDELYVVTARHVTERGYDLR
                     AKVRIKDLRTGAIKVAFLNLPRKRWVYHPNQGDRDTRSVDVAAMKINWLENHQVMTFL
                     YEMEGQKEGGGNQLSLQDPEPPQPILVFGFPSEIGFELTEQRPMGRVGIVSMVAGNKF
                     VKMEGKYVAERACLIDIGAFPGNSGSPVINQFSLQGEAQIKLLGVLIGTNPKRRYAVM
                     EPVSRVRETLEIARSQSLEGLTFWSLTEKNPERP"
BASE COUNT          997 a         1349 c         1262 g         1040 t
ORIGIN      
        1 aagcttccgc agcacgacgg ccagccgctc cttatcccgg ggatgaaggc ctaccgaggg
       61 atcgtccagg acatagaggg tgctggtgaa ggaagccccc agggccgtgg ccagggtgac
      121 ccgctgggtt tctccgcctg aaagggtccg ggactgccgc cccagggaaa tgtatcccaa
      181 tcccacatcc tgcagaaatt cgagccttcc ccggatttcc tggaggagaa ggtccgtcgc
      241 tcggtccccc cctggaggag tcctgtgatg gaagaattct tctgcttcgg cgatggacat
      301 cttttccact tccggaaggg tcttgtcgcc taggcgatag agaagtgcgg ggggcttcag
      361 ccttcctcct ccacaatcgg ggcaggggag gtaccgccga tagcgggcaa gaaaaatccg
      421 ggactgtttc ttgtacctgc gtccctccaa ccatttgaag agcccatgga tgccgtacca
      481 ggagccgttg ccctctccct cccatatcaa cctttgaacc ttggggtcca actccctcca
      541 gggacggtcg cccgggatct tcttccgctt cagaaaacgc agcaaatctt tctggcattc
      601 gaagaaggcc ggggtctgga aaggtttgat gcagccttcg gcgatggtca tccctgcgtc
      661 ggggatgacc aggtcggggt caatgtcgat gacccgcccg aatccatggc aggcctcaca
      721 ggccccgatg gggttattga aagaaaagat ggctggcgag ggagtcccgt agtcgaggcc
      781 gcacctctcg cagtgaagct tctcgctgaa gcgcaagggc tccctctcgc cttcgatcca
      841 tgcctccatg cgcccttcgc cgaaccgcag cgcggactcc accgagtcca cgatccttcg
      901 ccggcgtcca gggtcgacga ggagccggtc gaggaccacg gtgaccgctt cgtccctggg
      961 tttcagttcc gcttcttcca ccaggatcgg ccggccaccc tcgagaacgc gggcgaaccc
     1021 tgcctgccgg aagacgtcac gcagcgctgc ggggctgaac cggcctaccc gccgggtgaa
     1081 gcagatgagg gcgtttttgc cggctgacgc cgccagaagg gcatcgaaga tcatggaggg
     1141 agagtatcga tgcaccggac gcccgcatcc cttgcagtgg aggatggagg cccgggcgaa
     1201 gagggggcgg aggtagtcat cgatggaggt catggtcccg acggtggagc gggaagtccg
     1261 gaccggggcg gtgcggctga tggcgatggc cggaaggatg tcgtcgatcc gctcggcctc
     1321 cgggcggtcc agccgctcca ggaattggcg ggcgtaggga gaaaaggttt ctacatacct
     1381 ccggtaccct tcggcataga gaacatcgaa ggccagagag gatttgccgg ccccggcaac
     1441 tccggtgaca atggtcacgg cccccaccgg gacatgcacc gtgatcccct tgaggttgtt
     1501 ctgtcgcgcc ccttgaacgt atatcggact gacaggcatg gtctttatct ttccaatctg
     1561 cgaggatcct gaaatttcaa acccgcccct acatcattcc ttataccaga ccggacaatt
     1621 agccacttat gatgtaaatt agccgcaaaa gagcataggg aagaacgcag agcgctgatg
     1681 attttttcgt gggattttcc ggtcatcccg tcatagaatt aatgttagca ttttttgtga
     1741 ccgtcttcga gcgccccgat gaatgaaaaa atggcccaaa ctttcaaccg ctaccgggac
     1801 gtcctccccg attatgaagg gtttctctcg gctgtggagc agcccattcc cgtatcgatt
     1861 cgggctaaca ctttaaagat cgatcccggc ccatttcagg ctttcatgcg cgaacggggg
     1921 tacgatctga agccggttga gggtattgaa gaggcctttt ccctgcaagg cgtggagagt
     1981 cccggagcga ccctagaata tttttggggc tactatcagg tccaaggcct cacttccatg
     2041 cttccggcca aggtcctttc tccccggccg ggagagatca tccttgacct atgcgccgcc
     2101 ccgggcggga aaaccacgca tctcgcccag cttatggggg accgggggct ggtggtggcc
     2161 aatgatttta aaaaagaacg gacccatgtc cttcgttcgc acatcgaccg gctgggaatt
     2221 ctttcggtcc tcgtgtgttg ctattctgga caggccttcc cccttcgtcg gaagtttgac
     2281 cgggttcttt tggaccctcc ctgctcagcc gaggggacgt accgggccgg gctggcgccc
     2341 acccggagtg aaaatcccaa agtggcccag catctcgtcc gggtccagaa agggctcctc
     2401 caacaagggc tagccgttct ccggcctggc ggaaccctcg tctattcgac ttgcacctat
     2461 gcccccgagg aaaacgaagc agtggtcaac gaggcggtga aagaagggga ggcagaactc
     2521 ctcccgattt ctattccctt ttcccactcc agggggttgc catcctggaa ggaagaatcc
     2581 taccatcctg atatggtcaa aacggttcgt ctttatccgc atcaagtaaa ttcctggggg
     2641 ttcttcattg cccaccttcg caagcgaagc tgaacgaaaa gagattctgg attatttctc
     2701 gaatcggttt gctatcccgg agaaggcctt cgaaggattt tgctttctcc tgggaaaaag
     2761 aaaaatctgg gtggtccggg gtcatccgga attggaaaga ctcatgaaac aattgaagtt
     2821 cgagagtgcc ggcatccccc tcctccgcac caaaacttcc atgtggaagc cgaccacgac
     2881 cgggctgcac ctcttcggcc tgcatgcaac cagaaatgtc attgacctgg agggagacgc
     2941 tctgcaagct ttcctgaaaa gggaagacag cgatggttcc ctccccctcg agcccggatt
     3001 cgtgatcgtc cggtgcgaag gaaaggttct ggggtgcggg atttacggca aggggaagct
     3061 tcgctcccag cttcctgaaa agcgggggga accgttcagg ggaaagcttt acccgcaaga
     3121 cttttagagg cagccggatg ggaaatacgg acttcctgcc ttaaccaatc catcctgact
     3181 ccctatttgg catttaagca tggtctttct tcagctgctc ggcaatcgcc ttgaaatcat
     3241 atgaagggta gacttggtac cggaaggcgc cccaggattt ctgttccatg aagcggttca
     3301 ggttccgtat cttggccggg tccacgctca cgtgaagaat gaagccgaag gacatggcca
     3361 ctacccagga gacaagttcc gtcccatcgg gcggaaaatc ccggtagaag ccagtcttct
     3421 tgaggtgggc catgatctca tccagggtct tggactgatc atgatgcagg atgacggtca
     3481 atagcactct tggttccgca gccatgtcct ttcctccgag actatgagtc agaattcaag
     3541 aataaggtgt tggtcaacca gctcaacaga aatgcaaatc caaacctctc gcagggttaa
     3601 ggcctttcgg ggtttttttc agtcaaagac caaaaagtca atccttccag ggactggctt
     3661 ctcgctattt ccagcgtttc ccgaaccctg gatacgggtt ccatgaccgc gtaacgcctt
     3721 ttcgggttgg tcccgatcaa aacgcccagg agtttaatct gggcttcccc ttggaggctg
     3781 aattgattga ttaccgggct tccactatta ccggggaaag cgccgatatc gattaaacag
     3841 gccctttcgg ccacatattt gccttccatc ttcacgaatt tattcccggc caccatggat
     3901 acgattccca ctctgcccat gggcctttgc tcagtgagct caaacccgat ttccgaggga
     3961 aagccgaaga ccaggatcgg ttgaggaggc tccgggtcct gaagggaaag ctgatttccc
     4021 ccgccctcct tttgcccttc catctcatac aaaaaggtca tgacctgatg attctccagc
     4081 caattgatct tcatggccgc cacatctacg ctgcgcgtat ccctgtcacc ctgattcggg
     4141 tggtaaaccc aacgtttccg gggaaggttc agaaaagcca ccttgatggc cccggttctg
     4201 aggtccttga ttcggacctt ggcacgcaga tcgtatcccc tttcggtcac atgcctggcg
     4261 gtgactacat acaactcatc cgtccattgg acgaagaagg ccgttccatc ggatttgctc
     4321 tgctctttaa ccctaccggg gccctctgca gccatttttt cgacgtgctc taacctgacg
     4381 attagccggt tggatttgga gtaaaaactc tccttatcct cgcctcctcc ttccccgaag
     4441 cctcccagca ataagaagat tacagaaata aatagaaaag acctttttcc cccctgcatt
     4501 tttctccgcc tacaaccccc ttttccatgc gggtttcatg ctttcgcggt actgactgtg
     4561 tcctaggctt cttccccagc cgtacaataa aataatagca ttctcctcga gaacttcaaa
     4621 ggtccccccg caaaataagg agcactaa
//
LOCUS       DASPRR010000053         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_331091, whole genome shotgun sequence.
ACCESSION   DASPRR010000053 DASPRR010000000
VERSION     DASPRR010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331091"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VEL68_01955"
     CDS             <1..776
                     /locus_tag="VEL68_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXZ34763.1"
                     /translation="LVDLKMSSGTTLVRSEEGELAGYVFIDVTGRDIGSYVKDLKRVV
                     AEKVQVPPGYHLVWSGQYEYMERAGERLKIVIPLTLLIVFVLLYFNTAHVGKVFIVLL
                     AVPFSLIGAFWFIYLLGYNLSVAVWVGIIALAGVDAETGVIMLLYLDHAYEKWKNQGR
                     MRSLRDLQEAIREGAVKRVRPKMMTVMAIIMGLLPIMWSHGAGADVMKRIAAPMIGGI
                     VTSFILELVIYPVIFEIWRGRGFKKGFSSAENQAKPKLA"
     gene            806..1519
                     /locus_tag="VEL68_01960"
     CDS             806..1519
                     /locus_tag="VEL68_01960"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXZ34764.1"
                     /translation="MNMSKKALGQWVSVAFLLPSLLGGDMAKKGAEEGWLAWFGDEFP
                     EQNEPQALEEGPLPGWLSAITESIAREYGVSPRLVKFIIWTESQGNPRKVSAKGAKGL
                     MQLMPVVVQRYKVPDPYDPVANIRGGVQYLSNLLEDFCGDIPLALAAYNAGPTAVRKY
                     GGIPPFAETQAFVKKVSELFHFVETSPPISLAAPGTGEKKFVDQENPPQRMTYSGSPG
                     LLTFLLRRMNLEGVEVQIQ"
     gene            1516..2034
                     /locus_tag="VEL68_01965"
     CDS             1516..2034
                     /locus_tag="VEL68_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34765.1"
                     /translation="MNRWLWVETLFIAAFLYVPSAMAQHQHGGMSGMPGMEGEKKPES
                     TLGNEASPNRTFLLQGGVKAAFSIMAMAEHKKMLQDMRMKMEVDPQATHNIAVTLSDT
                     RTNLPITDAVVKMKVIDPKGKDQIKLLDFIAGMNQYAGDFTLSGKGRYQVLILFKAGG
                     KKEAAGFYYLLK"
BASE COUNT          548 a          570 c          654 g          501 t
ORIGIN      
        1 aattggtgga tttaaagatg tccagcggga ctaccctggt gaggagcgaa gaaggggaac
       61 tagccggcta tgtattcatc gatgtcactg ggagggacat cggaagctat gtgaaagact
      121 tgaagagggt tgtggctgaa aaggtccaag ttccccccgg ataccacctg gtctggagcg
      181 ggcagtacga atacatggaa cgggctggag aacggctgaa gatcgttatt cccctgaccc
      241 ttctcattgt ctttgtcctt ttgtatttca acaccgccca tgtgggcaag gtcttcatcg
      301 tgcttctggc tgttcccttt tcgttgatcg gcgccttctg gttcatttat ctcttaggtt
      361 acaatttgag tgtggcggtt tgggtaggca ttatcgcttt ggccggggta gacgccgaaa
      421 ccggcgtcat catgcttctc tacctagatc acgcctatga aaagtggaag aatcagggga
      481 ggatgcggag cctgagagac ctgcaggaag ccattaggga aggcgcagtc aagcgggtcc
      541 gccccaagat gatgaccgtg atggccatca tcatgggact gctgcccatc atgtggagcc
      601 acggggccgg ggcggatgtg atgaagcgaa ttgcggctcc catgatcgga gggatcgtaa
      661 cttccttcat cctggagctg gtcatttacc cagtgatctt cgagatctgg cgggggaggg
      721 gattcaagaa aggcttttcg agcgcggaga accaagccaa gccgaagctg gcctaaagga
      781 gatatgaacc taggaggagg gaataatgaa tatgtctaaa aaagcccttg gacagtgggt
      841 tagcgtcgct ttccttcttc catcgctcct gggcggggat atggcgaaaa agggagcaga
      901 agagggatgg ttggcgtggt ttggcgatga gtttccagaa cagaacgaac cccaggcact
      961 cgaggaaggg cctttgccgg ggtggttgtc ggcgatcacc gaatccattg cccgcgagta
     1021 tggcgtctcc ccccggttgg tcaaatttat catctggacg gaatcccagg gaaaccccag
     1081 gaaagtatcc gccaaagggg ccaaaggact gatgcaatta atgcccgtcg tggtgcaacg
     1141 ttacaaagtg cccgatcctt acgatcctgt cgccaatatc cgcggcggag ttcaatacct
     1201 cagcaatctc ctggaggatt tttgtggcga tatacccctg gccctagcgg cttataacgc
     1261 cgggcccacc gcagtgcgaa aatacggagg gatccctccc tttgcggaaa cccaggcgtt
     1321 cgtaaaaaaa gtgagcgagt tattccattt tgtggagaca tcacctccca tttctctggc
     1381 ggccccagga actggcgaga agaaatttgt agaccaggaa aatcctcccc agaggatgac
     1441 ctacagcggg tcccccggac tcctgacttt tctcctaagg cgcatgaatc ttgaaggggt
     1501 tgaggtgcag attcaatgaa caggtggcta tgggtagaaa ccctctttat cgcggccttc
     1561 ctttacgtcc cttcagcaat ggcccagcat caacatggcg ggatgtccgg catgcccggg
     1621 atggaagggg aaaagaaacc cgaatcgacg ttaggaaatg aagcatctcc caaccgtacc
     1681 ttcctgttgc agggcggcgt caaggctgcc ttttccatca tggccatggc ggagcacaag
     1741 aagatgcttc aggacatgag gatgaaaatg gaagtcgatc ctcaggcaac tcacaatatc
     1801 gcggtaaccc tcagcgatac ccggacgaac ctgcccatca ccgacgcagt ggtcaagatg
     1861 aaagtcatcg acccgaaggg caaagaccag atcaaactcc tggacttcat cgccggcatg
     1921 aatcagtacg cgggcgactt caccctgtcc ggcaagggcc ggtaccaggt cctgatcctt
     1981 ttcaaagcag gcggcaagaa agaagccgcg ggtttttatt acctcctgaa gtgatcgcca
     2041 ctcttccccc ctgattcaaa atgggcgaca gattgaatcg taaggacccg acttgggtaa
     2101 attcgctgct cctggcggca agcttcttgt caattatgaa aggcctctgg ccgggagtcg
     2161 cctatcatga ttggaagaaa atgaaccctt gcggagaggt tgtttattcg cgagtggatg
     2221 ggaagatgga aaatttcccg gccatgcgcg ccctggatga attcaagtct ccc
//
LOCUS       DASPRR010000054         6137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_331218, whole genome shotgun sequence.
ACCESSION   DASPRR010000054 DASPRR010000000
VERSION     DASPRR010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331218"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VEL68_01970"
     CDS             complement(<1..1314)
                     /locus_tag="VEL68_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXZ34766.1"
                     /translation="MKQHMPLGYGKWIRLPLMGMLGLISLAIFPLAPGWTQEKGVKVL
                     TREEALRIGLEKNKDIKKAQEYRNSVMGRYVEERAAALPQLQLSGGYNRDRDDATSNV
                     LHGLFPPEKETWGTEVGISQLIYSFGRVGAAIRAAKIGLSTAEDQLRIYQQAALRDVS
                     SAFDDVLLAKEIYALSLQNYDQKVRIQDETRRKHAAGVATDYDVLAADVAVENARPEV
                     IRRENQIRLTRERLRFLLGLEGEEVDAKGTFEETITPYPSYEKGVATAWKSRPDLSDL
                     QKRIGIAAELVKINDAGDKPRVDLKGSYGYKDINYFDVARGDGQVWSAGVFLTFPFFD
                     GMRSRGKTIQAKSDLSSLKIDEAKMKDSIALQVRDALNACREAEEIVKALSGTVRQAE
                     KLLSMAEKGYEYGVKTKLEVDDAQLSVIQAKGNLARARRDYSVARV"
     gene            complement(1311..4412)
                     /locus_tag="VEL68_01975"
     CDS             complement(1311..4412)
                     /locus_tag="VEL68_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXZ34767.1"
                     /translation="MFLSNLSIKRPIFATVMMLALVTLGIFSYRRLAIDMFPDVEIPV
                     VSVVTKFPGASPETVEREVSKRIEEAVNPISGVKHVLSTSRESVSNVVILFQLEVKIN
                     EAVQEARAKINAIRGDLPQAIEEPIIQKLDFAAFPIVSLAVRSQRLSPRDLTTLVDKK
                     VKRRFENISGVGKINLVGPSKREVNVNIDPLRLEALGMGVDEVIGGLQSENVNTPLGR
                     LTRGGTEYPLRISGKPDVVDQFKTMVIARRGGRPIPLAEVAQIQDGIEEQRSLALVNG
                     IPAIGLDILKQSGANTVGVVDRVKREIEILQRELPPGTTVEMVRDASIMIRDSVQDVE
                     ETLILGGILTIFIVFCFLNSWRSTVITGLTLPISVISSFTAMNFLGMTLNVMTLMALS
                     LAIGMLIDDAIVVRENIVRHLERGEDHFTAAQEGTSEIGLAVLATTLSVVAVFVPVAY
                     MKGIVGRFFFQFGITVAFAVLVSLFVSFTLDPMLSSRWVDPDIERKGKRHLLARVLDQ
                     FNHWFDRTADGYRGVIGWALDHRKTVLFLATAAFFAGLGVFYTLQTEFFPNFDKGEFQ
                     LNFKSAPDASIEETQNRISALLTALREIPEVQHVYATIGARDTDTVRDGLVFVKLVER
                     KERKRTQAEIQRDFRSRLEKIPGILPAILEWAGASSEKPLVVNIRGEDITLLKKYAAE
                     LKERIYQIQGITDLEVTLEQDIPEFRLKVDRERAVDAGVMTADIVRTVGALVGGQAVT
                     TYEDEDGDAVNVRVRLPLNLRQDPAQVERFRLAVQKPNAPPALVPLGNVVSYGMSTTP
                     AEINRQDLTRQVVITANLDNLPLGTAVEKVKEAGAKIPMAPGYRVVFSGEAEDMAESF
                     GYMGEALILAIIFVYLILAAQFESFIDPLAIMFSLPLSIVGMAGMLFFTRGTINIMSL
                     IGLIMLMGLVTKNAILLVDFTKTLRSRGIDRRQALITAGRTRLRPILMTTLAMMFGML
                     PLALALGQGAEMRAPMARAVIGGLITSTLLTLLVVPVVYTVLDDVGGWVRRRWEGTKE
                     SEG"
     gene            complement(4414..5586)
                     /locus_tag="VEL68_01980"
     CDS             complement(4414..5586)
                     /locus_tag="VEL68_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ34768.1"
                     /translation="MRPPFRNWAWTALVIFLGMVAGGCSTQAEDKKQTAKVSERPPVA
                     VEVTKVSASEVTGAIEVTGSLSYKFGADVKSEYTGIVTDVYVNEWVRVKKGDSLAKLD
                     TREAEVVLQKAKAAVEVARASILQAEVSQNRANREFERAQKLKETGLITQQAFDDART
                     ETEAAKARIEATKAQLKVAEEDVFYAQTRLSKSMIRSPIDGMVSLRNVNVGDFVGEMG
                     AKPMFRIVDTRILELTVTVPSGEMGSVRIGQPLQFSIAAFPGRNFVGRVMFINPVVNE
                     ADRSIKVVAEVENREDQLKGGLFVQGRIVTGRRTGVLKIPRAALLSWDVASKKGEVFA
                     VNQDVARRRPIQTGTILGDHVEIISGLAAGDLVVTRGGFNVKEGDKVNVTRINGEK"
     gene            complement(5583..>6137)
                     /locus_tag="VEL68_01985"
     CDS             complement(5583..>6137)
                     /locus_tag="VEL68_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXZ34769.1"
                     /translation="TDLFTQRGYAAATVREIVGAAGVTKPVLYYYFRNKEGIYLELMR
                     QAFARLDELIAGSMGDRGSATQKLLRLCDRVYTLFMENVKLARVMYSIYYGPHQGAPF
                     FDFDAYHLKFQEAVRGLIQEGIRKGEFKRGNPEDMTWAILGAINVAMEVHLGHVELAL
                     GREGLARVLRLVFQGISVKKGRRK"
BASE COUNT         1210 a         1878 c         1689 g         1360 t
ORIGIN      
        1 caccctggca accgagtaat cccgccgcgc ccgggccagg tttcccttgg cctggatgac
       61 gctcagctgg gcatcgtcca cctcgagctt ggtcttgacg ccgtattcat accccttttc
      121 ggccatggaa agaagtttct cagcctgccg gaccgttccg gagagggctt tcacgatctc
      181 ttcagcttcc cgacacgcgt ttaacgcgtc gcggacctgc agggcgatgg agtctttcat
      241 tttggcttca tcaatcttca gcgaactgag gtcgctcttg gcctggatcg tctttccgcg
      301 actgcgcatc ccgtcaaaga aggggaaagt caaaaagact cccgcgctcc agacttgtcc
      361 atccccccta gccacatcga agtagttgat atccttatac ccgtaagaac cctttagatc
      421 cactcggggc ttatctcccg catcattaat cttgacgagc tccgctgcaa tgccgattcg
      481 tttttgcagg tcggaaaggt cgggacgact cttccaggcc gtagccaccc ccttttcata
      541 ggaaggatag ggggtgatgg tttcttcgaa ggttcctttg gcatccacct cttccccctc
      601 cagccccagg aggaagcgga gtctttcccg ggtaaggcgg atctggtttt ccctgcggat
      661 gacctccgga cgggcatttt ccacggccac atccgcggcc aggacgtcat agtccgtggc
      721 caccccggca gcatgcttgc gccgggtttc atcctggatc cgtaccttct ggtcgtaatt
      781 ctgtaaggag agggcataga tttctttagc cagtaacaca tcgtcgaagg ccgaggagac
      841 atcccgcaga gcggcctgct ggtaaatgcg cagctggtcc tcagccgtag aaagaccaat
      901 cttggccgcc cggatcgccg cgcccactct tccgaaactg tagatcagct gggaaattcc
      961 tacttccgtc ccccaggtct ctttttccgg cgggaagagt ccatggagaa cattactcgt
     1021 ggcatcgtcc cgatcccggt tgtacccccc ggaaagctgg agttggggca gggcagcggc
     1081 tcgctcttcc acataacgcc ccatcacgct gttccggtat tcctgggctt tcttgatgtc
     1141 cttattcttc tccaggccga ttcggagggc ctcttcccgg gtcagcactt tcaccccctt
     1201 ctcctgggtc cacccggggg ccagaggaaa tattgccagg cttatgaggc ccagcatccc
     1261 catgaggggt agccgtatcc atttcccata tcccagaggc atgtgttgtt tcatccttcc
     1321 gactccttcg tcccttccca tctgcgccgg acccatcccc ccacgtcatc gagcaccgtg
     1381 tagacaaccg gaacaacgag caatgtcaat aaggtcgagg taatcaaacc cccgatgacc
     1441 gcccgggcca tgggcgcccg catctccgcc ccctgcccga gggccagggc taaggggagc
     1501 atgccgaaca tcatggccag cgtggtcatc agaatgggcc tcagtcttgt ccggccggcg
     1561 gtgatcaggg cttgcctgcg gtctattccc ctggaacgca gggttttggt gaaatccacc
     1621 aggaggatgg cgttcttggt aaccaggccc atgagcatga tgagcccgat gagggacatg
     1681 atgttgatgg taccacgggt gaaaaagagc attccggcca tacccacgat cgaaagggga
     1741 agggagaaca tgatggccag gggatcgatg aacgactcga actgagccgc cagaatgagg
     1801 taaacaaaga tgatggccag gatgagcgcc tcccccatgt aaccgaagga ttcagccata
     1861 tcctccgcct cgccggaaaa gaccacccgg taccccggag ccatggggat ttttgccccg
     1921 gcctccttca ctttttctac agccgttcca aggggtaggt tgtcgaggtt ggccgttata
     1981 accacctgac gggtcaggtc ttgtcggtta atctccgccg gcgtggtgct catcccgtag
     2041 ctgaccacgt ttcccagcgg gaccagcgca ggaggggcat tcggcttctg taccgctagg
     2101 cgaaaccttt ccacttgcgc cgggtcctgc ctgagattta ggggtaaacg gacgcgcaca
     2161 ttcacggcat ccccatcttc atcttcgtaa gtggtgacgg cctgcccgcc caccagcgct
     2221 cccacggttc ggacgatgtc tgcggtcatc accccggcat ccactgcccg ctcccggtcc
     2281 accttcaggc ggaattcggg aatgtcctgt tccaaggtta cttccaggtc ggtgatccct
     2341 tggatttggt agatcctttc cttcaattcc gccgcatatt tcttcagcag ggtaatgtcc
     2401 tccccccgga tgttgacgac cagaggcttt tcgctgcttg cccctgccca ttccaagatc
     2461 gctggaagga tgccggggat cttttccagg cggctacgaa agtcgcgctg gatctctgcc
     2521 tgggtccgct tccgctcttt tcgttcgacg agctttacga agaccaggcc gtctcgcacc
     2581 gtatccgtgt ctctggcccc gatggtggca tagacgtgtt ggacctcagg aatttctcgc
     2641 aacgccgtca gcaaggcgga gatacgattt tgggtctctt cgatggaggc atccggtgca
     2701 ctcttgaaat tcagctggaa ctcacccttg tcgaaattgg gaaaaaactc cgtttgcaga
     2761 gtgtaaaaga cgcccagccc cgcgaagaag gccgccgtgg cgagaaagag gaccgtcttg
     2821 cggtgatcga gagcccagcc gatcaccccg cgatatccat ccgccgtccg gtcgaaccag
     2881 tggttgaatt ggtcaaggac ccgcgccaga agatgccgtt tccctttccg ctcgatgtct
     2941 gggtccaccc agcgcgagga gagcatgggg tccagggtga aggaaacgaa aagagagacg
     3001 agcacggcaa aggccaccgt gatcccgaac tggaagaaga atctcccgac gattcctttc
     3061 atgtaggcca ccggaacgaa cacggcgacg acagataggg tggtggccaa aacggccagt
     3121 ccgatctcgc tcgttccttc ctgggcggcg gtaaagtggt cctcccccct ctcgaggtgt
     3181 cgtacaatgt tctcccgtac tacgatcgcg tcgtcgatga gcatgccgat ggccagggac
     3241 aaggccatca gggtcatcac gttcagggtc atgcccagga aattcatggc cgtgaaggag
     3301 gagatgaccg aaatgggcag ggtcagcccg gtgatgacgg tagaccgcca ggagttaagg
     3361 aagcagaaga cgatgaaaat ggtcaggatt ccgccgagga tcagggtctc ttccacatcc
     3421 tgcaccgagt cccggatcat gatcgaggcg tcacgtacca tttcgaccgt ggtcccggga
     3481 ggaagttctc tctgcaggat ctcgatctcc cttttcaccc gatcgaccac gcccacggta
     3541 ttggcccctg attgtttgag gatatccagg ccgatggctg ggatgccgtt taccagggcc
     3601 agcgaccgtt gttcctcgat gccatcctga atttgagcca cttcggccag gggaatcgga
     3661 cgcccgcccc gccgggcgat gaccatggtt ttgaactggt ccaccacatc gggctttcca
     3721 gagatgcgca ggggatattc cgtgcctcct cgggtcagcc ggccgagggg ggtattgacg
     3781 ttctccgact gcagaccgcc gatcacttcg tctaccccca tccccaaggc ttccaggcgg
     3841 agcgggtcga tattcacgtt gacctcccgc ttcgacgggc cgacgagatt aatctttccc
     3901 acgccggaga tgttttcaaa tcgtctcttg accttcttat cgaccaacgt tgtcaggtcc
     3961 cgcggggaga gtctctggga acgaacggcc agcgaaacga tggggaaggc ggcaaaatcc
     4021 agcttctgga tgatgggctc ctcgatggcc tggggcaggt cgccccggat ggcattgatc
     4081 ttggcccgcg cctcctggac cgcttcgttg atctttacct ccaattggaa aagaatcacc
     4141 acattggaga cactctcccg agaggtggat aagacatgct tgacgccgga gatggggttg
     4201 accgcttctt caatccgctt gctcacctcc cgttccactg tttcggggga ggcaccggga
     4261 aacttggtca ccacggagac gacggggatc tctacgtctg gaaacatgtc gatggccagc
     4321 cgccggtagg agaagattcc cagggtgacg agggccagca tcatgaccgt ggcaaagatc
     4381 ggcctcttga tggatagatt ggaaagaaac attctacttc tccccgttga tccgggtcac
     4441 gttcaccttg tcgccttctt tcacgttgaa ccctccccgg gtgacgacca ggtctcccgc
     4501 agcgagtccc gagatgattt ctacgtggtc tccaaggatg gttccggtct ggatcggtct
     4561 tcgccgggcc acatcctgat tgaccgcaaa gacctccccc ttcttactgg ccacatccca
     4621 gctgagaagg gcggcccggg ggattttcag gactccggtg cgtcttccgg ttacgatccg
     4681 gccttggacg aaaagtccgc ccttcagttg atcctcccga ttttccacct cggccacgac
     4741 cttgatggaa cgatcggcct cgttcaccac cgggttgatg aacatcaccc tgccgacgaa
     4801 attcctgccg ggaaaagcag cgatggagaa ttgaaggggc tgcccgatcc ggacgctccc
     4861 catctcgccg gaggggaccg tcacagtgag ctccagaatc ctcgtgtcca cgatgcggaa
     4921 catgggcttg gctcccattt cccccacgaa atcacccacg ttcacattcc ggagggagac
     4981 cattccgtcg atgggcgacc ggatcataga tttggaaagg cgggtctggg cgtagaagac
     5041 atcttcctca gccactttca gctgggcttt ggtggcttcg atccgggctt tcgcggcttc
     5101 cgtttccgtg cgggcgtcat cgaaggcctg ctgggtgatc aaacccgttt cctttagctt
     5161 ctgggcccgc tcaaactccc gattggcccg attctgggag acctcggcct ggaggatact
     5221 ggccctggct acttcaaccg cggccttggc cttttgcaag accacttccg cctcccgggt
     5281 gtcgagcttg gcaagggaat cgcccttctt gactctgacc cactcgttta cataaacgtc
     5341 ggtaacgatc ccagtgtatt ccgacttcac atcggcccca aatttatagg atagcgaccc
     5401 ggtcacctcg atcgccccgg tgacctctga ggccgagacc ttggttacct caacggccac
     5461 gggaggacgt tcagatacct tcgctgtctg ttttttatcc tcggcctggg tggagcatcc
     5521 gccggcaacc atccccagga aaatgaccag agcggtccaa gcccaatttc tgaatggtgg
     5581 tctcacttcc gtctcccttt tttcactgat atcccctgaa agacgagtct tagaacccgg
     5641 gcgagtccct cccgccctaa cgccaactcg acatgaccca ggtgcacttc catggccaca
     5701 ttgatcgccc cgagaatggc ccaagtcata tcctcagggt ttcccctttt gaactctcct
     5761 ttgcggatcc cttcttgaat gagccctcgg acggcttcct ggaatttcag gtggtaggca
     5821 tcgaagtcaa agaaaggggc tccttggtgg ggtccgtaat agatcgaata catcactcgc
     5881 gcgagtttga cattttccat gaagagggtg tacacccggt cgcagaggcg gagaagtttc
     5941 tgggtcgcgc ttccccggtc tcccatcgag ccggcaatca gttcgtccag cctggcaaag
     6001 gcctgccgca tgagttccag gtaaatccct tccttattcc gaaagtagta gtacaagacc
     6061 ggtttggtta ccccggctgc gcccacgatc tcccggacgg tggccgctgc atatcccctt
     6121 tgggtaaaca ggtccgt
//
LOCUS       DASPRR010000055         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1625210, whole genome shotgun sequence.
ACCESSION   DASPRR010000055 DASPRR010000000
VERSION     DASPRR010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625210"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..981)
                     /locus_tag="VEL68_01990"
     CDS             complement(79..981)
                     /locus_tag="VEL68_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXZ34770.1"
                     /translation="MSEIKSSSSDPVRAAIILPVGLTVMSFASIFIRFCDAPPLIIAA
                     YRLTLATLILLLLALPRTLRESRRLSRKEILPSLAAGLFLCFHFAFWITSLQYTTVAS
                     SVIFVTTNPIFVAVASTFILRERISLALLVSILMAVVGGTIIGWGDLGKGRDQLYGDF
                     LSLLGAVMATGYLLVGRRVRQKVSLTTYITLVYGGAALFLILLALFKGDAFFGYAPKD
                     YLLFLLLAVGPQLIGHSSLNWALRFFSATLVAVVILGEPIGASILAYFILGENPGINL
                     IWGGALVLLGIYLSTREEKKLGKI"
     gene            complement(974..2065)
                     /gene="ychF"
                     /locus_tag="VEL68_01995"
     CDS             complement(974..2065)
                     /gene="ychF"
                     /locus_tag="VEL68_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXZ34771.1"
                     /translation="MGFVCGIIGLPNAGKSTIFNALSSAKAQVASYPFCTIQPNQGII
                     PVPDKRLERLAQILKPGKVTPTILEFWDIAGLVKGASKGEGLGNQFLGHIRNVDALAH
                     VVRCFEAENVTHIYASLDPKRDIEIVQTELILADLQTVDKQLSKNLHQAKVGDKKSSA
                     EVPVLQAIQDRLAKGRPAAGLTFPEEGQKVFRSLELLTAKPVFYVANASEKELKERRW
                     ISPVEELAGREKAPVVILCGDLEAEMAELSSAERDEFLREMGLQEPALNQLIRVGYGL
                     LKLITFYTTVGPELRAWTVPHGTHVPQAAGKIHTDMERGFIRAEVISFEEFVAAGSSA
                     AARDKGLIRVEGKEYQVQDGDILYIRFHV"
     gene            2387..>2903
                     /locus_tag="VEL68_02000"
     CDS             2387..>2903
                     /locus_tag="VEL68_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXZ34772.1"
                     /translation="MRNWRRTSTPVLLILGGLSLLDPAGAMAQKVHSIDLTTAIAEVA
                     QQTMPSVVHIEVIQRVQVPSPVFPFEGDSFFRFFFGAPPRPRFDRREVRGIGTGMIID
                     GDGHIITNNHVVNGATKVQVKIASGEEMEAKIVGADPKTDLAVIKIKLPSNTPFLKFG
                     DSDKLRVGEWVV"
BASE COUNT          675 a          754 c          857 g          617 t
ORIGIN      
        1 gagattgccg cgcccttcgg gctcctaatg acatgtgaca ttatttaatg cgtttgtact
       61 agttccgggc gctacggttc agatttttcc cagttttttc tcctcccgtg tcgacagata
      121 gattcccagc agaaccaaag ccccgcccca tatcaggttg ataccagggt tctcccccaa
      181 gatgaaatag gccagaatgg atgccccgat gggttcgcct aagatgacaa ccgcaaccag
      241 ggtggcggaa aaaaatctca aggcccagtt cagggaagag tgcccgatca gctgaggccc
      301 aacggccagg aggagaaaga gaagatagtc cttgggagca taaccgaaga aggcatcgcc
      361 tttgaataag gccagaagga tcaagaaaag tgccgcccct ccgtaaacca aagttatgta
      421 agtggtgagg ctgaccttct ggcgcactct ccttcccacc aagagatagc ccgtggccat
      481 gaccgctccc aggagggaca gaaaatcgcc gtagagttgg tcccggcctt ttcccaggtc
      541 cccccagccg atgatcgttc cccccaccac agccatgagg atggaaacaa gcagagccag
      601 ggaaatcctt tcccggagga taaaagtgga ggcgacggcc acaaagatgg ggttagtggt
      661 tacaaagatc accgagctgg ctacggtcgt atattgcagg gaggtgatcc aaaaagcaaa
      721 atgaaagcag aggaagaggc cggcggccag agagggaagg atttccttcc ggctcaacct
      781 gcgggactca cggagggttc tcggcaacgc gaggaggaga agaatcaggg ttgccagggt
      841 tagacgatag gcggcaataa tgagcggagg ggcatcgcag aaacggatga aaatggaggc
      901 aaaggacatg accgtcaggc ccacgggcag gatgattgcg gcccggacag ggtcggaaga
      961 ggaggatttt atttcagaca tggaaccgaa tatagaggat atcgccatcc tggacttggt
     1021 actcttttcc ttcgacccgg atcagtccct tatccctggc tgcagccgag gaaccggcgg
     1081 cgacgaattc ctcgaaagaa atgacttccg cgcgtatgaa cccccgctcc atatccgtgt
     1141 gaatcttgcc cgcggcctgg ggaacgtggg tcccgtgggg tacggtccag gcccgaagct
     1201 ccgggccgac cgtggtgtaa aaagtaatga gtttcaggag gccatatccc acccggatga
     1261 gctgattcag ggcaggctcc tgaagcccca tctcccgcag aaattcgtcc cgctcggccg
     1321 aagagagctc ggccatctcg gcctccaagt cgccgcagag gatcaccaca ggggcctttt
     1381 cccgtcccgc cagttcttcc accggagaaa tccagcgccg ctccttcagt tctttttcgc
     1441 tggcattggc cacgtagaag actggtttgg cggtgagaag ctccaaggaa cggaagacct
     1501 tctggccttc ttcgggaaaa gtcaggccgg ctgccggacg tcccttggcc aggcgatcct
     1561 ggatggcctg caggaccggc acttcggcgg aggatttctt gtcccccact ttggcttggt
     1621 ggaggttttt gctgagctgc ttgtccacgg tctgaaggtc ggccaggatg agttcggtct
     1681 ggacgatctc gatatcccgc ttggggtcca ggctggcgta aatgtgggta acgttttctg
     1741 cttcgaagca gcggaccacg tgggccaggg cgtccacatt tcggatgtga cccaggaatt
     1801 ggtttcccag gccctccccc ttgctggctc ctttgaccag gccggctatg tcccagaatt
     1861 ccaggatcgt gggggtgact tttcctggtt tcagaatctg ggcaagcctt tccagccgtt
     1921 tatcgggaac ggggatgatt ccttggttgg gctggatggt gcaaaatgga taactggcca
     1981 cctgggcttt ggccgagctg agagcgttga aaatcgtgga tttcccggca ttgggcaggc
     2041 cgatgatgcc gcatacgaaa cccaaggagt cctcccaaga cctggttggc ggaaatttcc
     2101 gtcgatcatt atactgaaaa gaggccaggg gagcaaggga tgtcactttt cggggtgagc
     2161 gggggaatat tcgtcaattt cctcgggatt ctaaacccag ggaagagcct atttgacatt
     2221 gaatcattag gagaataaaa tggtttataa atatgggaat tttttaacct tttcccgaat
     2281 gtcgccctgc ttatgacggg tttcggggga gaaaagctct catatccgtt cgaggcaatg
     2341 ggagacgatt gaatcagcga tacctgtata aggttaggat aaaataatga gaaattggag
     2401 aaggacctcg acccccgttc tgcttatcct gggcggtctt tctttgcttg atccagccgg
     2461 ggcaatggcc cagaaggtcc attccatcga cttgactaca gccatcgccg aggtggccca
     2521 gcagaccatg ccctccgtgg tccacatcga ggtgatccag agggtccaag tcccatcacc
     2581 ggtcttcccg ttcgaggggg attctttttt tcgctttttc ttcggagctc ccccaaggcc
     2641 ccgttttgac cggagagagg tgcggggaat cgggacgggg atgatcatcg acggcgatgg
     2701 acatattata accaacaacc acgtggtcaa tggggccaca aaagtccagg tcaagatcgc
     2761 ctcgggggaa gaaatggagg ccaagatcgt gggggcggac cccaagaccg acttggcggt
     2821 catcaagatc aagcttcctt ccaatacccc tttcctgaaa ttcggcgact cggataaact
     2881 gcgagtggga gaatgggtgg tgg
//
LOCUS       DASPRR010000056         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_173421, whole genome shotgun sequence.
ACCESSION   DASPRR010000056 DASPRR010000000
VERSION     DASPRR010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173421"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VEL68_02005"
     CDS             complement(<1..1149)
                     /locus_tag="VEL68_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ34773.1"
                     /translation="MAGEIKRSVCPYDCPDTCGLLVEVADGKAVRVSGDPEHPFTRGT
                     LCPKMNQYERTVHSPLRLTHPLMRTGPKGSGQFRPISWSEAVRQVADRWRKIIAQHAG
                     QAILPYSYAGTMGLVQRNSGHPFFYRLGASRLDRTICAPAKEAGWKAVMGNTAAPHPD
                     ELAFSDLVILWGINALATNIHQLHAVREAKKKGAQVWLIDTYETPTARIADRTFVVRP
                     ASDGALAMGIMSALVRDNLLDHPFLATSVQGFVELKNKILPEMTLERVSSLTGLPSGT
                     IESLARALGKARAPFIRIGAGLSRYGNGGMNVRSIVCLPALVGAWGKKGGGAFVGTST
                     AGAFAMGEITREDFMAGETRAVNMNQLGHALNHLDNPPIQSLYVYHSNP"
     gene            1469..2467
                     /gene="galE"
                     /locus_tag="VEL68_02010"
     CDS             1469..2467
                     /gene="galE"
                     /locus_tag="VEL68_02010"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HXZ34774.1"
                     /translation="MGETNHQILVTGGAGYIGSHVVKELIRRGYRAVVYDNLQTGHRK
                     AVKNAPLIQGDLADKERLIRAFQSYSIQAVMHFAADCLVNESMKEPLKFINNNIRNGL
                     HLLEAMNQWGVKKFVFSSSCAVYGEPVKVPIPEDHPCLPTNVYGETKWVFEKFLQAYH
                     DAQKLDFISLRYFNAAGADPDGELGEDHQKETHLIPLAFKALLGKNRLSIYGTDYDTP
                     DGTCIRDYIHVMDLAQAHFLALERLLQGGESATYNLGNGNGYSVREVIAMAEKVSAKK
                     MPLIEAPRRAGDPAQLVASSEKIKKELGWTPKYPKLESIIQSAWNWHRTHPNGYAD"
     gene            complement(2592..>3773)
                     /locus_tag="VEL68_02015"
     CDS             complement(2592..>3773)
                     /locus_tag="VEL68_02015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXZ34775.1"
                     /translation="NMGLLTLTSKGFAWFSKGHPWVFRDDLEKIQDAPAGGIVSLERR
                     NGQFLAQGFYSDRSKIAFRLISRSREPIDRDFWKGRFQRAAQFRQLAVEKTNAYRVVY
                     GESDGIPSLIVDRYGDHWVMQTLSQGADRATGLFADLLAELFQPYSIILRNDLAVRDL
                     EGLPQEKKMILGDSSERVEVFEGDVRYQVDPWNGQKTGAYLDQRENRLRAGRFLRGKV
                     LDAFCYQGLFAIHAARSSAQVTGVDSSLEALEQAKENARLNDLANIEFRRENVFDFLK
                     GEAERGSSYDGINLDPPAFAKSRADVNAALRGYKELNLRAIRLLNPGGVLVTSSCSYN
                     LSESKFLDLLKECARDARADLWIMEKRGQGADHPVLLSFPESFYLKCLFLQKGPLPYP
                     S"
BASE COUNT          840 a         1043 c         1052 g          838 t
ORIGIN      
        1 cggattggaa tgatagacgt acaggctctg gatcggggga ttgtccaggt ggttcaaggc
       61 atggccgagc tggttcatgt tcactgcccg cgtctcccca gccatgaaat cttcccgggt
      121 gatttccccc atggcaaaag cccctgccgt ggatgtgccg acgaaggccc cgcccccctt
      181 ctttccccag gcgcccacca gcgcggggag gcagacgatg gaccggacgt tcatcccccc
      241 gttcccgtaa cgggaaagac cggccccgat gcggatgaag ggtgcccggg ccttgcccaa
      301 ggcccttgcg agggattcga tcgtccccga gggaaggccg gtcagagagc tgaccctctc
      361 cagggtcatc tcgggtagga ttttgttttt cagttcaacg aatccctgga ccgacgtggc
      421 caggaaggga tgatccagga ggttgtcccg gaccagggcg gacataattc ccatggccag
      481 ggccccatcg ctcgcgggcc tgaccacgaa agtccggtcg gcgatccgcg ccgtgggggt
      541 ttcgtaggtg tcgatgagcc agacctgggc gcccttcttt ttggcctccc ggaccgcgtg
      601 gagttggtgg atgttggtcg ccagggcatt gattccccag aggatgacca gatcgctgaa
      661 ggccagttcg tcagggtggg gggccgccgt attgcccatg accgctttcc aacccgcttc
      721 cttggccggc gcacatatgg tccggtccag gcgcgaggcc cccaagcggt agaagaaggg
      781 atggccggaa ttacgctgaa cgagtcccat ggtcccggcg taggagtagg ggagaatggc
      841 ttggcccgca tgctgggcga tgatcttcct ccatcggtcg gccacctggc ggacggcttc
      901 gctccaggag attgggcgaa actggccaga gcccttcggg ccggttcgca ttaagggatg
      961 ggtgagacgg aggggagagt gcacggtcct ttcgtattgg ttcatctttg gacagagggt
     1021 cccgcgggta aagggatgct ccgggtcccc cgagactcgg acggctttcc catccgccac
     1081 ctcaaccaga agcccgcagg tatccgggca gtcatagggg cagaccgatc gcttgatctc
     1141 tccggccaca aagacctcct aaaggaagta agtattcaat gattcttttc tatattagcc
     1201 gttttttcca gtggccacaa gtttggattc gtcgatgcga cgatattcct cctacagcct
     1261 gcggcctgta acccaggagt tgcttcctgt ttctatgcct tatttaatcc aggatccatc
     1321 atccctgaat cctttgactt gactaggtct tggattttca ataaaattgc gcagtatcat
     1381 gatcttgaat cctgtatcca gtttccttta tccagcagcc agtatccttt agcccatgac
     1441 ctatttcatt ttgacctgag ctctgcgaat gggagagacc aatcatcaaa ttcttgtaac
     1501 cggcggtgca ggctatatcg ggagccatgt ggtgaaagaa cttatccgca ggggctaccg
     1561 agccgttgtt tacgacaacc ttcaaaccgg gcaccggaag gcagttaaga atgcccccct
     1621 aattcaggga gacctggcag ataaggagag gctcatccgg gcttttcaat cgtattccat
     1681 acaggccgta atgcatttcg ccgccgattg tctggtgaat gagtccatga aggaaccatt
     1741 gaaatttatc aacaataata tcagaaacgg cctgcacctc ctggaggcaa tgaatcagtg
     1801 gggggtgaaa aaattcgtct tttcatcctc ctgtgccgtc tacggagagc ctgtaaaagt
     1861 cccaattccg gaagatcacc cctgccttcc cacgaatgtt tatggagaaa cgaaatgggt
     1921 ctttgaaaaa ttcctgcagg cctatcatga cgctcaaaag ctcgatttta tctccctcag
     1981 gtattttaat gccgcagggg ccgacccgga cggagaactg ggggaagatc atcaaaaaga
     2041 aacccacctg atcccgctcg cgtttaaagc tcttctcggg aaaaatcgac tttccatcta
     2101 tggcaccgac tatgataccc ccgacggcac ctgtatccgg gactatatcc atgtgatgga
     2161 cctggcccag gctcattttt tagccttgga aagattgctg caaggaggag aatccgccac
     2221 ctacaatctg ggaaatggca atgggtattc tgtcagggaa gtgatcgcca tggccgaaaa
     2281 ggtctcggcc aagaagatgc cccttatcga agctccaaga agggcgggcg atccagccca
     2341 gctggtagcc agttcggaaa agattaaaaa agagctgggc tggacgccta agtatccgaa
     2401 gttggaatca atcattcaat ccgcctggaa ctggcatcga acccacccga acggttatgc
     2461 agactaggac ccgtttcaag cggtctgtga actgtaacct gaaacctgag acctgtagcc
     2521 tgtgacctca tccgttagtc ctctctgcgg tctctttgcg cgcgatcaga gcagtcgaga
     2581 gaaattgcat ctcaggaagg atacggcaag gggccttttt ggaggaaaag gcatttcagg
     2641 tagaaacttt ccggaaagga aaggagaacc gggtgatcgg caccctgtcc tcgtttctcc
     2701 attatccata ggtcggctct tgcatcccgg gcacattcct tcaggaggtc aaggaatttc
     2761 gattcggata gattgtaaga acaggaagaa gtcactagaa ccccccccgg gttgaggagg
     2821 cggatggccc gcagattcaa ttccttgtat ccgcggaggg cggcattcac atccgcccgg
     2881 ctcttggcaa aggctggcgg gtcaaggttg atcccatcat agctgcttcc ccgttccgct
     2941 tctcccttca aaaaatcgaa gacgttttcc cggcgaaatt cgatattggc cagatcgttc
     3001 aagcgagcat tctctttggc ctgttccagt gcctccaggg aggagtcaac gccggtgacc
     3061 tgggcggaag accgggcggc gtggatggcg aataatccct ggtaacagaa agcgtcgagg
     3121 acttttcccc gcaggaaccg tcctgctcgg aggcggtttt ctctttgatc caagtaggcc
     3181 ccggtttttt gaccgttcca agggtccacc tggtaacgga catccccttc aaagacctcg
     3241 acccgttccg aagaatcccc cagaatcatt tttttctcct ggggcaatcc ctccaaatcg
     3301 cgaaccgcca aatcgtttcg tagaatgatg gaataaggct gaaaaagctc cgccaggagg
     3361 tcggcgaata gaccagtggc ccggtccgct ccctgggaaa gggtctgcat cacccagtgg
     3421 tctccatacc ggtcgacgat gagggaagga atcccgtccg attccccgta aactacccgg
     3481 taggcgttgg tcttttccac ggcgagctgc cggaattggg ctgcccgctg aaagcgtccc
     3541 ttccagaagt cacggtcgat gggctcccgg ctgcgggaga tcaggcggaa agcgattttt
     3601 gagcggtcgc tgtagaaacc ctgggccaga aactgcccgt ttcgcctctc caaggaaacg
     3661 atcccgcctg ccggggcatc ctggattttc tccaaatcgt cgcggaagac ccaaggatgg
     3721 cccttcgaaa accaggcgaa gccttttgat gttagcgtga gaagacccat att
//
LOCUS       DASPRR010000057         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_173992, whole genome shotgun sequence.
ACCESSION   DASPRR010000057 DASPRR010000000
VERSION     DASPRR010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173992"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VEL68_02020"
     CDS             complement(<1..1502)
                     /locus_tag="VEL68_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34776.1"
                     /translation="MRRYLIFSLIIVAFLIGGLKLYFVYSGLDIRPNPGEPGWSSGDL
                     VHLLPTVSHDRILIKASFKKALTQAPLLEVDSQVFPGKRTDTQGFFWLFDAPGLKPNQ
                     KYELVVKDSSGQSLCDPWTLSTFPAPEDQPESLRLLIFTGAGGHDAHIEWFGSGPLPL
                     STRIRLLKRALSLNPDALISSGDQIYYDLRYDKSARVMGDSARSIAYAGRFDPGLPIL
                     GTPNEEVLKKAVGPQIAFLYGTLCRSIPTFFLLDDHDYFENDIATERDGFDIRLLLLG
                     WRSPFYKGGVSFPPDPFMLDLARSAQKLYLPEFLPDENRPMDLPGSNAPDRTPGISEC
                     YGTLRYGQLFEGLLYESRRYVTLTGKDAVFIHTKAEDWLIARMAAEQTTHLVNIPAVV
                     FGWSAGKWMEWYPDVHDKAGELTTVLAKYQWQEGWLDQHNRVLKAASSMKRSLPLFIC
                     GDLHSQAWGKILKSRDLDLRVNPIISVVSGSLGTGPRGFPSGFRGLTAKPP"
BASE COUNT          492 a          620 c          685 g          492 t
ORIGIN      
        1 gggggcttgg ctgtcagacc gcgaaacccg gaaggaaaac cccggggacc ggttcctagt
       61 gatccggaaa cgacagagat gatcgggttg acccgcaggt ctaaatcgcg gctcttgagg
      121 attttccccc aggcctggct gtggaggtcc ccacagatga aaagggggag gctcctcttc
      181 atggaagaag cggccttcaa gacccggttg tgctggtcga gccatccttc ctgccactgg
      241 tatttggcga ggactgtggt gagctccccg gctttatcgt gcacgtccgg ataccactcc
      301 atccacttgc ccgcgctcca gccgaagacc accgccggga tgttgaccag gtgagtggtc
      361 tgctccgccg ccatgcgggc gatcagccaa tcctcggctt tcgtgtggat gaaaaccgcg
      421 tccttgccgg taagggtgac atacctcctg ctttcataga gcaacccctc aaacagctgg
      481 ccgtaacgga gggtcccgta gcactcggat atgccggggg tccggtccgg ggcattgctg
      541 ccgggcaggt ccatcggccg gttttcatcg ggcaaaaatt cagggaggta gagtttttgg
      601 gccgaccggg cgaggtcaag catgaaaggg tccgggggaa aggatacacc ccctttgtag
      661 aaaggcgagc gccaacccag cagcaggagc cggatatcga agccgtctct ctcggtggcg
      721 atgtcgtttt cgaagtaatc gtggtcatcc aagaggaaaa aggtgggtat ggaccggcaa
      781 agggtgccgt acaggaaggc gatctggggc ccgacggcct tcttcagcac ttcttcattc
      841 ggcgttccca ggatgggaag gccggggtca aaccgaccgg cgtaggcgat ggaacgggcc
      901 gaatccccca taaccctggc ggatttgtcg taccgcaggt catagtagat ttgatccccg
      961 ctggagatca gggcgtcggg gttcaaggaa agggctctct tcaggagccg gattcgggtc
     1021 gagaggggca gggggccgct cccgaaccat tcgatgtggg cgtcgtgtcc gcccgctccg
     1081 gtgaagatga gcagccgcaa actctccggc tggtcctcgg gggcgggaaa ggtggagagg
     1141 gtccagggat cacagaggga ttgcccggag gaatccttga ccaccagttc gtatttttgg
     1201 ttgggcttca gtcctggggc atcgaatagc cagaagaagc cctgggtatc ggttcgtttt
     1261 cccgggaata cttgggaatc cacttccaag agaggcgcct gagtcaaagc ttttttaaaa
     1321 gaggccttga tgaggatacg gtcatggctc accgtgggaa ggaggtgaac caggtcccct
     1381 gaactccagc cgggttcccc tggattgggc cggatgtcca gtccggaata gacgaagtag
     1441 agttttaaac ctccaatcag gaacgcaacg atgattaacg aaaagatcag gtaccgtctc
     1501 atgcgccacc tccggagaaa cgggttatga gacccccaga tgatttctac gggaagagag
     1561 gactttggga ttccagacgg tgagcaatag gtaacagaaa aagggttgcg ggtcaaggat
     1621 tacttcacgg gctctttcaa ccccgatttt ccgggagtgg tcgcggccgc gccttccagg
     1681 cttgcccacc cccagttttt cgtgtttgtc ggggttcagg gtggagaaat ttggatcatc
     1741 gggctccgcg ggtgcttatc cttccatcaa actcctcccc atgttccgca gggagaggcc
     1801 tgagaggaga tacctccaag caaaaaagag aaccaaagca tttttccgtt tcaatccccc
     1861 caggacaggc ccgattcaat gggagaacca ccgcctcaga tgatgatcca aatttctccg
     1921 ccctgaaccc catgaggtcc cccctccttt ccagagatct tattttcgaa ggactctttt
     1981 ttaagaactg gggttgggca agatactggt ttctggttgg gggtcgctaa atgccgatcg
     2041 ttctttaagc tctgggcatt tcaagcactt tagcgcattt taggcactct aggcattcta
     2101 ggcaatctag cgcattctct gcaagaagga atatctcact catctttcca cccgtaatcc
     2161 cccaccaaag ggacgaagac acatccggtc acatcttctt cgctgaattg gtccccttcc
     2221 cgggtcagct tgtacagggt ttgggtgaac cgcggacctg tggggatgac cagccttccc
     2281 ccttcggcc
//
LOCUS       DASPRR010000058         9640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_500917, whole genome shotgun sequence.
ACCESSION   DASPRR010000058 DASPRR010000000
VERSION     DASPRR010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500917"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEL68_02025"
     CDS             <1..213
                     /locus_tag="VEL68_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ34777.1"
                     /translation="HIPIPANERALKELGNRQAANMVLLGAVVKTVGLVSEEALLQSL
                     TKRVPPSFLETNKKALTLGFGLAKNG"
     gene            620..976
                     /locus_tag="VEL68_02030"
     CDS             620..976
                     /locus_tag="VEL68_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34778.1"
                     /translation="MPFQTRYIFSAAMDVDPDKDALFNEVYDKEHVPLLVKVPGVVSV
                     ARFKKREVTLIIGGQRKKILVENEPTYNAFYEIESPEVLVSDAWAKAVDQGRWPGQVR
                     PYTRNRRHVLYERLVK"
     gene            1158..2423
                     /locus_tag="VEL68_02035"
     CDS             1158..2423
                     /locus_tag="VEL68_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34779.1"
                     /translation="MRKTVLFAFVFMQVLGLVVIPAFAAEEVKIGVLYPMSGPAAQAG
                     LDDKNALELALDIINTTKYKHLNLPLSKSEGLPNLKGAKMSAIFADHQGKPDLGLSEA
                     ERLITQEKVVALFGCYHSSVTETASMVAERMKIPFFNAESSSPRLTRRGFKWFFRSSP
                     HDETFSEGMFKCLVDLEKKRGIKFQTIAVAYEDTLYGKDSSRIEKELAAKAGYKVVAD
                     IPYRRAATSLTSEVQRLKAAKPDVFFPTSYASDAILLCKASKDLDFNPSAIMAQNAGH
                     TDPSFIEAVGKDADGICSRTEFSLDLAKHKPMLTEINQLFKERSKRDFSGTSARAFAG
                     FFVLADAINRAGSTNPEAIRDALTKTNIPADQLITPWRGVRFDETGQNILVDAIVIQY
                     QGGQPHTVWPFDLAAKEIMYPIPKWSERK"
     gene            2573..3844
                     /locus_tag="VEL68_02040"
     CDS             2573..3844
                     /locus_tag="VEL68_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34780.1"
                     /translation="MKKGLFIGFIAAGLLTLGLASPAPAQEVIKIGSIYPLTGPVGQA
                     GINNKNAIELAMEIINTTKYKHLNVPLAGAGGLPNLKGAKVQVVFSDHQGKPDLGLSE
                     AERMITQEKVVVLTGCYHSNVTETASMVAERMKFPFYNFESSSPKLTQRGYKWFFRSS
                     PHDETFSEAMFAFVKEYGQKKKIQFKTIAVMYEDTLYGKDSARIEKLLADKAGYKVVA
                     DIPFRRGATSLTSEIQKLKAASPDIFFPTCYISDAILMTKTSKELDYNAPMVMAQNSG
                     HTEPSFLEAVGKDADGIASREEFSLDLAKHKPMLTEINQMYKDRYGRDFSGPAARAFV
                     GFLALCDAINRAGSTNPEAIREAIKKTNIPGDKLITPWRGIQFDENGQNILVDAIVIQ
                     YQDGAKRYTIYPFNLATREIVYPIPRWSERK"
     gene            3920..4789
                     /locus_tag="VEL68_02045"
     CDS             3920..4789
                     /locus_tag="VEL68_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017972132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34781.1"
                     /translation="MLTTEIIIQTIVSGILMGCIYALVAVGLCLIWGLMEIVNFAHGD
                     YLMMAMFTSFWAYTLAGIDPLLSLPLCLALLFAIGWFTYYIIIKKLLRATFLAQILAT
                     FGLGVFVRYLAQYMWSPDFRTIKEPLLAGNIGIGGIFVGTPQLVASIICVIAFVFLYV
                     LMNKTELGFAILATSEDREAASLMGIDSHKMFALGWGVGCGCLGVAGAMMANFFYIFP
                     EVGFNFGLIAYVAVALGGFGSVPGSFLAGIIIGVVEFLSGLIIAPAFKYAFVFLIYLL
                     VVFIKPMGLFGRY"
     gene            4819..5778
                     /locus_tag="VEL68_02050"
     CDS             4819..5778
                     /locus_tag="VEL68_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34782.1"
                     /translation="MKNKNWVWLIIGAILVLLFPVFFTLPFPRHLMIMIFLYATMGLA
                     WNLIGGYAGQISLGHAIFFGTGAYTSTLMLMKLGITPWLGMLVGGLLSVGIAFVIGYP
                     CFKLAGHYFAIATIAIGEIAMVLMINWDWAGSAVGLTLPILEEGLWNFEFHTTKAPYY
                     YIAFGIMVFALFVTYGVDRSKLGFYFKAIKGDLEGARSLGINTHRYKFYALALSAFLT
                     SICGSFYAQYVLFIDPDSTFPLLLSIILCLVATLGGVGTILGPVIGAAILIPISEFTR
                     IYLGGGGKGTDLILYGFLIMIISIYQPFGVIGIAKRLRKRESR"
     gene            5778..6515
                     /locus_tag="VEL68_02055"
     CDS             5778..6515
                     /locus_tag="VEL68_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34783.1"
                     /translation="MACLEVRGLVKDFGGLRANDHIDLRVDEGEIVGLIGPNGAGKTT
                     LFNCIAGFYPPSAGAVQFKGEEITGLPSNETCLRGIARTFQLVRVFKDLTVLDNVMIG
                     AFNRTQSAAEARRKAMEVLDFCGLMSKKGMLAGGLTIADKKRLEVAKAFATGPSLLML
                     DEAMAGLNQTETAEAVDLVKKIRKSGVTVLLVEHVMEVVMPISNRVVVLDYGRKIAED
                     TPEKVINNEEVIKAYLGDKYHASRRAH"
     gene            6493..7200
                     /locus_tag="VEL68_02060"
     CDS             6493..7200
                     /locus_tag="VEL68_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34784.1"
                     /translation="MLRVERIKVSYDEVPALHEVSFKVEAGQIVSIVGANGAGKSTIL
                     KTISSVMRPDEGAIYFENQRVDQTPPHKVVDLGIAQVPEGRRLFARLTVTQNLTLGAY
                     TRKSPEHRESTLKKIFKLFPVLEERKSQAAGTLSGGEQQMLAIARGLMSKPKLLMLDE
                     PSMGIMPKLITEIFDMVQRVNKEEGLTILLVEQNVQEALEIAHYAYVLQTGRMVMEGK
                     PADLLKTDMIKKAYLGL"
     gene            7495..8688
                     /locus_tag="VEL68_02065"
     CDS             7495..8688
                     /locus_tag="VEL68_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXZ34785.1"
                     /translation="MEGIDDLLASLYQHFNEDTVLDLARKLIAVPSHAASGEEKTAAV
                     LARFLERAGLIPQFQRVEDVGVNLIATLPGEGQETALILNGHLDTVPPSSSMAYPPFQ
                     AVVRDGLLWGRGSCDMKGGLAAMACAMAMIRAANLPLKRSVIFTAVASEEQGNRGTAA
                     LIRKGLRAEWAVVGEPTGLDLVIAHKGVDRYRFQIEGRAAHESLPERGTNAIVAAAHL
                     IAALDRNLFSKVMREKHSLLGPATYNIGTIQGGVSRNTVPDRCTFQVSKRWLPGDSPA
                     AIRRELEKAIREVETEAKVSFVREPDMERVPHPPLELGPDHPLTRTLAMVIHRVIGRM
                     PVFSAMPAFTDAALFQDAAIPAVVFGPGDLGLAHSDGESIPISQLGQATRVYAALAVI
                     AANSL"
     gene            complement(8746..>9640)
                     /locus_tag="VEL68_02070"
     CDS             complement(8746..>9640)
                     /locus_tag="VEL68_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXZ34786.1"
                     /translation="QCHLVMRQGHLTRCTVNPTLGEPDECRLDRTEHPQRVVVVGSGP
                     AGMEAAVTAAARGHQVTLCEKDREVGGNLRPGSIPFFKEDLRRYFDYIRRRLESSGVQ
                     VLLNREGDVEFLKTLAPDVVVVATGAEMSPLSLPGGERALPVVSVLADRAPLGKRVLI
                     IGAGFVGCETGWHLAYEGRQVQLVDLLPEAELLAEEHYVNRATLFYQLNRAGVSLLCS
                     ASPKEITDRGVLVTLADGRERLLAADSVISCVGFRPRRKLYQELLREGNGWDVYPAGD
                     CVKVENLHHAIQAAHQLARHL"
BASE COUNT         2093 a         2745 c         2707 g         2095 t
ORIGIN      
        1 cacattccta ttccggccaa tgaacgggcg ttgaaggagt tggggaaccg ccaggcggcc
       61 aacatggtcc tcctgggggc tgtggtaaag accgtaggat tggtcagcga agaggctctc
      121 ctccagtccc tgaccaagcg ggttccgcct tccttcctgg aaacgaataa aaaagcactt
      181 accctggggt ttgggctggc gaaaaacggt tgatcctact gggtcattcc ttcctgaatt
      241 cgattggctc cccctcctcc gtttttttcc cctcttcatc cctttatcca ggcctttttc
      301 ccgcccctgc ccgtcttttg catcctctcc ccctttatct ggaaggtgtc cctgaagagg
      361 atgtgcaggg aatggccggc tggcccgccg ggtcagcgag acgccgggtc cttcgctcgg
      421 cttaaattcc cttgacgcac aatcgccttt ttaatatatt caacccaact caaagcgagt
      481 tcttatcaac catccgaggc cgatgcgaat tcgtcccggc acgggttgct gccccgcaga
      541 gacttggggc gagttggctc ggcaggcaaa gagattcccc cgagccgacg caccggacct
      601 ccagaaagga gagtcgatta tgccattcca gaccaggtat atcttcagcg ctgccatgga
      661 cgtggacccg gataaggatg ctctgttcaa cgaggtctac gataaggagc acgtgccgct
      721 tctcgtgaag gttccgggag tggtctcggt ggcgcgattt aagaagcgag aagtcaccct
      781 gatcattggg ggccagcgca agaagatctt ggtcgagaac gagcccacct ataacgcctt
      841 ttacgaaatc gagagtcccg aagttctggt gagtgacgcc tgggcgaagg ccgtcgacca
      901 gggtcgctgg ccgggacagg tgcgcccgta caccagaaac cggcggcacg tgctctacga
      961 gcggctcgtg aaataggcta cgcttgccct gatgcgaatc ccggcggagg ccctccgggg
     1021 agacgagtcc acgaccttgc ggcctggatt gatgggaccg gcatgctgta ttttcgcccg
     1081 cagaggtctc gcttatatgc cgtttttggg gtatgcaacc caagacgagt tcttatcctt
     1141 atcaaaagga ggaaaatatg agaaaaacgg tattgtttgc attcgtcttt atgcaggttc
     1201 tcggcctggt tgtcattcca gcatttgctg ccgaggaagt aaaaattggg gttctatacc
     1261 ccatgtctgg tccggcggct caggcgggcc ttgacgacaa aaatgccctc gagctggcct
     1321 tggacatcat caataccacc aagtacaagc atctaaacct cccgttatcc aaatccgagg
     1381 gcctccccaa tctgaaaggg gccaaaatga gtgccatatt tgccgaccat cagggtaaac
     1441 ccgacctggg gttgagcgaa gccgagcgcc tgatcactca ggaaaaggtg gtggccctct
     1501 tcggctgtta ccacagttcc gtaaccgaga cggccagcat ggtggccgag cgcatgaaaa
     1561 ttcctttctt caacgccgaa tcgtcttccc ccagactcac ccgccgggga ttcaaatggt
     1621 tcttccgcag ctcgccccac gatgagacct tttctgaagg catgttcaaa tgcctagtcg
     1681 acctggagaa gaagagggga atcaaattcc aaaccatcgc ggtggcgtac gaggatactc
     1741 tctacgggaa agacagcagc cgcatcgaga aggagctggc cgcgaaagcc gggtacaaag
     1801 tcgttgcgga tattccctac cggagagccg ccacctccct gacctctgag gttcagaggc
     1861 tgaaagctgc caaaccggat gtcttcttcc ccacctccta cgcctcggat gcgattctgc
     1921 tctgcaaagc ctctaaagac ttggatttta acccatcggc catcatggct cagaatgccg
     1981 gccacacgga cccctccttc atcgaagcgg tggggaaaga tgcggatggg atttgcagcc
     2041 gcacggagtt ctctttggac ctggcgaaac ataagcccat gctcaccgag atcaatcagc
     2101 ttttcaaaga acgttccaag agggatttct ccgggacctc ggcccgggcc tttgcgggct
     2161 ttttcgtctt agccgatgct ataaaccgag caggctccac caatcccgaa gccatccgcg
     2221 atgctctgac caagaccaac attcctgccg accaactgat cactccctgg cggggggtga
     2281 gatttgatga aaccggacaa aatatcctgg tagatgccat cgtcatccag tatcagggcg
     2341 gacagcctca cacggtatgg cctttcgacc tggccgccaa ggaaatcatg tacccgatcc
     2401 caaagtggtc ggagagaaaa tagtagacct caagacctaa gggtccaagc tatcgatatg
     2461 tcggtggcgg gggatcaacc aggggtccct tttcaagttg cttttagatt ttgcttgtgg
     2521 taaatagtcc gcatttggac aaggagagcc agatagaaag gagggtgcaa aaatgaaaaa
     2581 aggattgttc atcggattca tcgctgccgg gctcttgact ctgggcctcg cttctcccgc
     2641 accggcccag gaagtgatca aaatcggttc catatacccg ttgaccggcc ccgtgggtca
     2701 ggccggcatc aacaacaaga acgccatcga gctggccatg gagataataa acaccacgaa
     2761 gtataaacac ctgaatgtgc ccctcgcggg ggccggcggt ctccccaatc tgaagggagc
     2821 caaggttcaa gtggtgttct ccgatcacca gggaaaaccg gacctgggcc tgagcgaggc
     2881 cgaacggatg atcacccagg agaaagtcgt agtcctgact ggatgttatc acagcaatgt
     2941 gaccgaaacg gccagcatgg tggccgagcg gatgaagttc cctttctata actttgaatc
     3001 ctcttccccc aaactgaccc agcggggata caagtggttc ttccggagct ctccccatga
     3061 tgaaaccttt tccgaagcca tgttcgcctt tgtgaaagag tacgggcaaa agaagaaaat
     3121 ccagttcaaa accattgccg tgatgtatga ggacaccctc tacgggaagg acagcgcccg
     3181 cattgaaaag ctgctggctg acaaggcggg gtataaggtt gtcgccgata ttcccttccg
     3241 ccgcggggca acttccctca cctcggaaat ccaaaaatta aaagcggcca gcccggatat
     3301 tttcttcccc acatgctaca tctccgatgc cattctcatg accaagacct ccaaggaact
     3361 ggactataac gctcccatgg taatggccca aaattccgga catacggaac cgtctttcct
     3421 tgaggcggtg gggaaggatg ccgatggcat tgcgagtcgg gaagagttct ctttggacct
     3481 ggccaagcat aagccgatgc tcaccgagat caatcaaatg tacaaggatc ggtatgggag
     3541 ggatttcagc ggccccgcag cgcgcgcgtt tgtgggcttt ttggccctgt gtgatgccat
     3601 caaccgggcg ggttccacga accccgaggc catccgggaa gcgatcaaga agacgaacat
     3661 cccgggggac aaactgatca ctccctggcg ggggattcag ttcgacgaaa acggccagaa
     3721 catattggtc gatgccattg tcatccagta ccaggacgga gccaagcggt acacgatcta
     3781 tccctttaac ctcgccaccc gggagattgt ataccccatc cccagatggt cggaacggaa
     3841 atagggaaat aggataagat cgaatcgggg ggcgggagaa cctggccccc gattcgattg
     3901 gctggagggt gggacgccta tgttaacgac ggaaatcatc atccaaacga tcgtcagcgg
     3961 gattctcatg ggatgcatct acgccctcgt cgcggtggga ctatgtttga tctggggcct
     4021 gatggaaatc gtgaatttcg cccatgggga ctaccttatg atggccatgt tcacctcgtt
     4081 ctgggcttac acccttgctg gtatcgaccc gttgctttcc ctgccccttt gcctggccct
     4141 ccttttcgcg atcgggtggt ttacctatta catcatcatt aagaaactcc tcagagccac
     4201 cttcctggcg cagatcctgg ccactttcgg cctgggcgtt ttcgtgcgct acctggccca
     4261 atacatgtgg tctcccgatt tccggaccat taaagaaccc cttcttgccg gaaacatcgg
     4321 aatcggcggg atcttcgtcg gcacgccgca actggtggcc agtatcatct gtgtcatcgc
     4381 tttcgtcttt ctctatgtgc tgatgaataa aacagagctg ggtttcgcca tcctggcgac
     4441 gagcgaagac cgggaagctg cgagcttaat gggcattgac agccataaaa tgttcgccct
     4501 gggctggggg gtcggttgcg gctgcttggg ggtggccggg gcgatgatgg cgaatttctt
     4561 ctatatcttc cccgaggtgg ggttcaactt cgggctcatc gcctatgtgg ccgtagccct
     4621 gggcggattt ggttcggtgc ccggctcctt cctggccggt attattatcg gggtggtgga
     4681 atttctgagc ggcttgatta tcgccccggc cttcaaatat gccttcgttt ttctgatcta
     4741 tcttctggtg gtattcatca aacccatggg gctcttcgga aggtattaac ctgatctttc
     4801 aaaaggatgc caagggaaat gaagaacaag aattgggtat ggttgatcat cggagccatt
     4861 ctggtgttac tctttcccgt gttcttcacc ctcccattcc cccgccatct gatgatcatg
     4921 atctttctct atgcgaccat gggattggcc tggaacttga tcggagggta tgccgggcag
     4981 atttcactcg gccatgccat ttttttcggc accggggctt atacctccac cctcatgctc
     5041 atgaagctgg gcatcactcc ctggttgggg atgttggtcg gtggccttct ctccgtggga
     5101 attgccttcg tcattggcta tccttgcttc aagctggcgg gccattactt cgccattgcc
     5161 accatcgcca tcggggaaat cgccatggtt ttgatgatca actgggactg ggcgggaagc
     5221 gccgtcggct tgaccttgcc gattctggaa gagggtttat ggaacttcga atttcacacc
     5281 accaaggccc cttactacta catcgccttc gggatcatgg ttttcgctct cttcgtcacc
     5341 tatggggtgg accgctcgaa gctgggtttt tatttcaagg ccataaaggg agacctggaa
     5401 ggggctagaa gcctgggcat caacacccac cggtacaaat tctacgccct cgccttgagt
     5461 gcctttctga cctccatctg cgggtccttc tatgcccagt atgtcctttt tatcgacccg
     5521 gacagcacct tccctcttct cctgtcgatc attctttgcc tggtggccac cttggggggt
     5581 gtggggacca tcctcggacc cgtgattgga gccgccatcc tgatccctat ctccgaattc
     5641 acccggatct atctgggcgg aggggggaaa ggaaccgatt taattcttta cggcttcctg
     5701 attatgatca tctcgattta tcagcctttc ggagtcattg ggatcgcgaa gcgcttacgg
     5761 aaaagggaga gccggtgatg gcctgtctcg aggtaagagg actggtgaag gactttgggg
     5821 gcctgcgggc caacgaccac attgatctgc gggtcgacga aggagagatc gtgggtttga
     5881 tcgggccgaa tggggcgggg aagacgaccc ttttcaattg tattgccggt ttctatccac
     5941 cgagcgcggg agccgtgcaa ttcaaagggg aagagattac cgggcttcct tcgaatgaga
     6001 cctgcctgcg ggggattgcc cggacctttc agctcgtccg ggttttcaag gatctgaccg
     6061 ttctggacaa tgtcatgatc ggcgccttca accgcactca gtcagcggcc gaggccagaa
     6121 ggaaagcaat ggaagtcctg gatttctgcg gcctcatgtc caagaaggga atgctggccg
     6181 gcgggctcac catcgccgac aagaaaaggc tggaggtggc caaggccttt gccaccggcc
     6241 ccagcttgct catgctggac gaggccatgg ccgggctgaa ccagaccgag accgcggagg
     6301 cggtggacct ggtgaagaaa atccgcaaat ccggggtcac cgtcctactg gtggaacacg
     6361 tgatggaagt agtcatgccc atttccaacc gggtggtggt cctggattat ggccggaaga
     6421 tcgccgagga cactccggaa aaggtcatta acaacgaaga agtgatcaag gcctacctgg
     6481 gagataaata ccatgcttcg cgtcgagcgc attaaagttt cctacgacga agtcccggcc
     6541 ctgcacgagg tctccttcaa ggtcgaggcg gggcagatcg tctccatcgt gggcgccaac
     6601 ggcgccggaa aatccaccat cctgaagacc atctccagcg tcatgcgccc cgacgagggg
     6661 gccatctact tcgagaacca aagggtcgac cagactcccc cccacaaggt cgtggacctg
     6721 gggatcgccc aggtccccga agggcggcgc ctcttcgccc gcctgaccgt aacccagaac
     6781 ctgactctgg gcgcctacac ccgaaagtct cccgagcatc gggaatccac cctgaagaag
     6841 atcttcaagc tctttcccgt cctggaggag cgcaagagcc aggccgccgg aaccctctcc
     6901 ggcggggagc agcagatgct ggccattgcc cggggtctca tgtccaagcc caagctgctc
     6961 atgctcgacg agccgtccat ggggatcatg cccaaattga tcaccgagat cttcgacatg
     7021 gtccagcggg taaacaagga ggagggcctc accattctcc tcgtggagca gaacgtacag
     7081 gaggccctgg agattgctca ttacgcctac gtgctccaga ccggccgcat ggtcatggag
     7141 ggaaaaccgg cggacctgct gaaaacggat atgattaaaa aggcatattt agggttatag
     7201 gtaagaggtt agaggcaaga gataaaagct taaaggtcta aggttagggc taagggcaag
     7261 gttaaagaag gactgggtgc ggggcgcaag ggaaaaagta cgaggaacga aattcggaga
     7321 taaaggaagt tcgattccgg ataccagaca aaagatgctg gatggggcat tttagcagtc
     7381 tgcggaaaaa ctccaatttg aagtcctatt tatatcctta tttcttaaaa tttcagctat
     7441 cctccctgaa attaatacct aaggtaattc cctgagattt ccggagcccc tttgatggaa
     7501 gggattgatg atcttttagc gagcctttac caacatttca acgaggacac cgttctggac
     7561 ctcgcccgca agttgattgc cgtgcccagt catgcggctt ccggcgaaga gaaaacggcc
     7621 gccgtgcttg cgcgattcct ggaacgggcc ggccttatcc ctcaattcca aagagttgaa
     7681 gatgtggggg tcaatctcat tgccactttg ccgggagagg gccaggagac ggcgctgatt
     7741 ctgaacggtc acctggatac ggtaccccct tcgtcgtcca tggcctatcc gccgttccag
     7801 gctgtggtcc gcgacggctt gttgtggggg cgcggctcct gtgacatgaa aggcggcctt
     7861 gccgccatgg cctgcgccat ggccatgatt cgcgccgcca atctgccgct caagcgctcg
     7921 gtcattttta ccgccgtagc ttccgaagag cagggcaatc gggggactgc ggccttgatc
     7981 cgaaagggcc tgagagccga atgggcagtg gtcggtgagc ccaccgggct tgatctggtg
     8041 attgcccaca aaggcgtgga ccggtatcgc ttccagatcg aaggacgcgc ggcgcatgag
     8101 agtctgccag agcgtggcac caacgcgatt gttgccgctg cccatttgat cgccgcactt
     8161 gaccggaatc tgttctccaa ggtcatgcgc gaaaagcatt ctcttttggg ccctgccacc
     8221 tacaacatcg ggactatcca gggcggggtc agccgtaaca cggtccctga tcggtgcacc
     8281 tttcaagtct ccaaacgatg gctgcccggc gattcgcctg cggccatccg aagggaactg
     8341 gagaaagcca ttcgcgaggt cgagacggag gcgaaggttt ccttcgttcg ggagcccgac
     8401 atggagcggg taccccatcc cccgcttgaa ttgggtcccg atcaccccct tacacgaaca
     8461 ctggccatgg tgatccatcg ggttatcggc cgcatgccgg tcttctccgc catgccggcc
     8521 ttcaccgacg ccgccctgtt tcaggatgca gcgatccccg cggttgtttt tgggccaggg
     8581 gacttgggat tggcccacag cgacggcgag agcattccga tttcccagct tggacaggca
     8641 actagagtgt atgccgccct tgccgtgatc gctgcgaata gcctctgaaa aagacccgga
     8701 aaaatccggt gaccagcagg gtccgagcca ggcttttcga aggtcttata gatgccgtgc
     8761 cagttgatgc gccgcttgaa tggcgtgatg gagattttcc actttgacgc aatcgcccgc
     8821 ggggtagaca tcccagccgt ttccctcccg gaggagctcc tgataaagct tccgccgagg
     8881 cctgaaacca acgcaggaaa tcacgctgtc tgcggccagc agtctctccc gaccgtccgc
     8941 cagggttacc aaaactcctc ggtcagtgat ttccttcggt gaggcgctgc agagcaaaga
     9001 aacacccgcc cgattcagct gatagaacag ggtggcccgg ttcacgtagt gctcctcggc
     9061 cagtagctcg gcctcgggga gaaggtcgac cagctgcacc tgccgaccct cgtaggccag
     9121 gtgccagccc gtctcgcatc ccacgaaccc ggccccgatg atcagcaccc gctttcccaa
     9181 aggcgctcga tccgcgagga cacttactac cggaagcgcc ctttcaccgc cgggcaagct
     9241 cagcggactc atttctgcgc ccgtggcaac tacgacgaca tcgggggcca gggtcttgag
     9301 aaattcgacg tctccttccc ggttgaggag cacttggacc ccgctgctct ccaaccggcg
     9361 gcggatatag tcaaaatacc gccgaaggtc ctccttgaaa aagggaatcg acccggggcg
     9421 aagattccca cccacctcgc ggtccttttc gcacagcgtc acctggtggc cccgggctgc
     9481 cgcggtgact gccgcttcca tgccggccgg accggaaccc accaccacca cccgttgcgg
     9541 gtgttccgtt cgatccaggc ggcattcgtc cggctccccc agagtagggt tgacagtgca
     9601 tcgggtcaaa tgaccctggc gcataaccag atgacactgg
//
LOCUS       DASPRR010000059        10190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1950502, whole genome shotgun sequence.
ACCESSION   DASPRR010000059 DASPRR010000000
VERSION     DASPRR010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950502"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VEL68_02075"
     CDS             <1..250
                     /locus_tag="VEL68_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXZ34787.1"
                     /translation="QLIIHRADPTSVERQIQRTRAVKAARDNLKAQKALDELCRIAET
                     KENLMPCFIQAVKAYASVGEITEALKKVFGEFKEPVNI"
     gene            311..1825
                     /locus_tag="VEL68_02080"
     CDS             311..1825
                     /locus_tag="VEL68_02080"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ34788.1"
                     /translation="MNIAEILPNTAKRLPDKTAFVFNDRPTKYKTLQDLTEAFAHGLQ
                     KLGVKKGDRVAVFAPSSLEFVVSWFSVVKLGAVFVPVNIMLKARESKYILENAEVNTL
                     IVHQTQGEIIQSIRPGLKFLKNLIVIGNQPLPGSLLFSALLAEKPGRDMIIDCVPDDT
                     ATMIYTSGTTGFPKGAMITHGNFDSNVKGIIVALNLDEGMVRVSVTPLFHAMGLTVNM
                     LAVVMLGATAVIQAKLDFEEFLKANERYGATMISGAPTLHYMLVNDPRTAHYNLSTWK
                     VGMSGSAPLPVDVLKKFEEKFKIPMVEAYGLTEVTTAATSNPVFGVRKPGSVGLPFTD
                     LEVKVFDELEREVPAGEVGEVVIRGSAVMKGYYNNPEATTETLKGGWLHTGDLGKKDE
                     DGYVYILDRKKDMIICSGYNVYPREVEELLHTHPAVMEAAVIGIPDPKRGESPMAFII
                     PRPGKKVTEEELIHFCKENLANYKVIKAVKFVEEFPRNPNRKVLKRELREMLKS"
     gene            1923..3065
                     /locus_tag="VEL68_02085"
     CDS             1923..3065
                     /locus_tag="VEL68_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ34789.1"
                     /translation="MDFSLREDQRIFKDTFRRFAEAEIGPLVEEAEETETFPLRLFPQ
                     MGKLGYLCPRYSENYGGAEIDKVSEVLMREEISRVSQGIASSWSAHSHLGTFPVYHWG
                     SEEQKQKYLLPAIRGEKIFAFGLTEPNAGSDVKAIETTARRDGDGYVINGRKIFITNG
                     NICDFLTLIAYTDKSRGYRGISIFLVEKGTPGFIVTRKLKKEGVRSSETAELLFEDCR
                     IPARNLIGEEGSFYRVMETLNEGRIGVAGNCVGMAQGAYEAAVKYARERVQFGRPIAQ
                     FQAIAFKLADMATEIDAARLLVLRAAWMMDQGKNPIQEASMAKLFASEVAVRVSKEAM
                     QVHGGYGQMREFPVGRFHRDALVYVVGEGTSEIQRQIIAKQMGMGG"
     gene            3085..4650
                     /locus_tag="VEL68_02090"
     CDS             3085..4650
                     /locus_tag="VEL68_02090"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXZ34790.1"
                     /translation="MVRSNQMLFGYLADKNAKYYPDKIFLIDENRRCTFRKFNSRTNA
                     LARGLLNAGIKPGDRVAVLNHNSVELFEVYFGAMKAGGIPVPINVRLAPPEVAEILQD
                     AAPSLLLVQKNYLSKIPPKGPGQTLSRVYAIGGSGTLPDYESLIGNYPTSRVETAADD
                     DDPAILIYTSGTTGKPKGVMLTHRNLLADVWASCVSRRLAPDDVALVTAPLYQAGAFG
                     SMISNVFRGNTIVILDGFDPPKVLATIEREKVTTALFVPAMILKLLEFPDSGRYDLRS
                     MRTVVYGAAPMPVGALKQAMERFRWDFMGACGATETGPGYIAFLDRENHYLDGSPQME
                     KRLQSIGKEGINDEVRIFDDEDRELPPGEVGEIVLRGPNIMKGYWGKPRETAEALRSG
                     WYHTGDLGYIDADGYIYIVDRKKDLIISGGFNVYPKEIENALDAHLAVLESAVVGIPH
                     PRWGETPLAFVVLKKGAPAPELEALMGFLRERLADYKLPKGGIQFTQELPRNASGKVL
                     KRELRAQYTPKPQ"
     gene            4673..5881
                     /locus_tag="VEL68_02095"
     CDS             4673..5881
                     /locus_tag="VEL68_02095"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ34791.1"
                     /translation="MKGTQTASRRILEGIRVLDLSTLITGPYCASLLGDLGGEVIKVE
                     HPRGGDGGRQVGSTFLEGESALFLAINRNKKDLTLALDKPAGQEILRRLIARADVLIE
                     NFRPDIGRQYQLDYEQVRAIRPDIIYLSITGFGEEGPYRLKPGTDHIFQGLSGLATIS
                     GKLGQGPIRVGTPVVDMTASLYSCLGVVSALLHRQRTGQGQQICVNLLDAAMCVQSPL
                     FTEYFITGHRPTPTGNASPFAYPVDIFPSADGNVSIGAFNDKFWRNLCRALNLDSLAD
                     DPWFNTPEKRLSNREELGSLLSKVLATRKTREWLKILEEADVPCGPVHDYDAVFSDPQ
                     VLHNQLVKTLPHSRLNEVRTLGNPISFSQTPAREERSAPLLGEDTDSILHSLGYKPEE
                     VKEFREKKII"
     gene            5893..6951
                     /gene="dctP"
                     /locus_tag="VEL68_02100"
     CDS             5893..6951
                     /gene="dctP"
                     /locus_tag="VEL68_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ34792.1"
                     /translation="MKNGRNPFSLLVALILVPILFLAGSSLSVAQAQPAKTVSWKLQA
                     AYPPPEKIMGHWGAYGQAAEVARQVKERTQGKFDIKVYPPDALFKAMEAPDAVKRGAV
                     EMILSNGAYHVGILPEANLDYGLPYGVKTSQEAARLLFESEYFRVLRKAYLEKHQVLL
                     LGLTSTSAYNYLTRFPIHKMEDMKGKKIRTSGAYGKVALAHGATPVNLSPAEQYMALQ
                     RGTVDGTIFPAYAGITYKMFEVAKYHSWPPIYALIGASLVANLSAFNKLPKEYQHILQ
                     EEVTKMVKYTYDVSGPALEKIAQEEGRKQFGAEAVFISDAEFLKFREAVKPLWDEWEK
                     KSDYCAQLVKIAKDFAHK"
     gene            6980..8035
                     /gene="dctP"
                     /locus_tag="VEL68_02105"
     CDS             6980..8035
                     /gene="dctP"
                     /locus_tag="VEL68_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ34793.1"
                     /translation="MNRKKICAGFIVLIVSVFFLLWAGNPQAFAQAKPSKVFQWKIQS
                     AWPPPEKFMGYWGGFGQVSEIARQVKARTNGGLDIKAFPPDALFKVLEAPDAVKRGAI
                     EMICSSGPYHINILPEALVEYGLPYGVKTSEQIAKLILETEFVSILRKAYAEKHNVYM
                     LGVCSASSYNYITRFPVQKLEDMKGKKIRATGTSARIATAHGAIPVSLSGAEQYMALQ
                     RGTVDGTIYPPYAGLSYKMFEVAKQVSWPPIFAASNINYLVSMNAWHQLPKEYQVILQ
                     EEVANMAKYAYHESGPTLEKIAREEGKKQYGAESTWLSDTEFARFQQAVMPMWEEWEK
                     KSPYCAQLVKIAKEFVK"
     gene            8102..8665
                     /locus_tag="VEL68_02110"
     CDS             8102..8665
                     /locus_tag="VEL68_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXZ34794.1"
                     /translation="METLARVLDGIDRLNEWLGKTFSILAIVLTLVVVYDVVLRYFFA
                     RPTIWGLELSCILLAYLTFMGGGYAFLHGGHVKVDFFYQKWTGRTKATVDLLTYTLII
                     AYCSVLVWQGGAVAWEAIQEARVSTSAWAPPLWPSQIMVPIGALLVGLQALAHWIRDF
                     RIAWSEKNDLASKVFTGEGGIFEKKKE"
     gene            8712..10022
                     /locus_tag="VEL68_02115"
     CDS             8712..10022
                     /locus_tag="VEL68_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ34795.1"
                     /translation="MDPAWLTLLLFGSLVLFLLLGLPIAFTLGGLALIFTLWLWGPGG
                     LLMLASHAYGESMNFILVSAPLFILMAVVLEGSGIAEDLFETMYLWFGGLPGGLLTGT
                     IIICAIFAAMSGISGVATVTMGLIALPAMLKRGYDRKMCIGGIMCGGALGIIIPPSVI
                     AILYGSITGTSVGKLFMGGFIPGIMIAGVMIFYTTVKCVRNPKYGPPVPVEERGNFRA
                     KMASLKFIIFPLLLILLVLGVIYSGICTPTEASAIGAFGALICSLIKRNLTWKSLKAA
                     TIRTLNITCMALWIVFTAKSFSSIYTAGGASDFILSLVQNLAIPPLMMIGIMQFIFFI
                     LGMFIDPVGMCMICSPVFLPIVTNLGFDPTWFGILFILNCGMAYITPPFGFNLFYMRA
                     IAPADCTMGEIYTSVWPWVICEAIVLILVMFFPIIGMYLPNQMG"
BASE COUNT         2261 a         2849 c         2958 g         2122 t
ORIGIN      
        1 ccagctaatc attcaccggg cagacccgac atccgtggaa aggcagattc agaggacgag
       61 agcggtaaag gctgcacgcg acaatcttaa ggcccaaaaa gcgctggatg agctgtgccg
      121 gattgcagag acgaaggaaa acctgatgcc ctgtttcatc caggctgtca aggcttacgc
      181 ctctgtggga gagattaccg aggctttgaa gaaggtcttt ggggagttca aggaaccggt
      241 gaatatttga aaacgctata cgcatagcgc ctagggcatg gcgtttcttt atttcgggag
      301 gagaaaagag atgaatattg ccgaaattct tcccaatacc gccaagcgtc tgccggataa
      361 aaccgctttc gttttcaacg atcggcctac gaaatataaa acgctccagg acctgaccga
      421 agccttcgcc catggattac agaagctggg ggtgaagaaa ggagatcgcg tggcggtctt
      481 cgcgcccagc agcctggagt tcgtcgtctc ctggttctcc gtggtcaagt taggagcagt
      541 cttcgtcccg gtaaacatca tgctgaaagc acgggagtcc aagtacatcc tggaaaacgc
      601 agaggtaaat accctgatcg tccaccaaac ccagggggag ataatccaat ccattcgccc
      661 gggcctgaag ttcctgaaga acctgatcgt catcggaaac cagcccctgc cgggctccct
      721 cctcttttca gccctcctgg cggaaaagcc cgggagggat atgatcatcg attgcgttcc
      781 agatgacacg gccaccatga tctacacctc gggcacaact ggctttccca agggagccat
      841 gatcacccat gggaacttcg attccaacgt aaaagggatc attgtggcct tgaacctgga
      901 cgaagggatg gtccgcgtgt ccgtgacccc tctcttccat gccatgggat tgaccgtgaa
      961 catgctggcg gtggtcatgc tgggagccac ggcggtcatc caggccaagc tggactttga
     1021 ggaattcctc aaagccaatg aaaggtatgg tgccaccatg atcagcgggg cgccgactct
     1081 tcattatatg ctggtgaacg atccccggac ggcccattac aatctttcca cctggaaagt
     1141 gggcatgtcg ggaagcgcgc cgcttcctgt ggatgtgctg aaaaagtttg aagagaagtt
     1201 caagatcccc atggtggagg cctatggatt gaccgaagtc accacagctg ccacatctaa
     1261 tcccgtcttc ggggtgcgca agccgggctc ggtgggcctt ccctttaccg atctggaagt
     1321 gaaggtcttc gatgaactgg agcgggaggt tcccgcgggc gaggtcgggg aagtggttat
     1381 ccggggctcg gcagtgatga aggggtatta caacaacccg gaggcgacca cggagactct
     1441 taagggtggc tggttgcata cgggagacct ggggaagaag gacgaagatg gatatgtgta
     1501 cattctcgac cggaagaagg acatgatcat ctgcagcggc tacaacgtgt atccccggga
     1561 ggtcgaagag cttctccaca cgcacccggc ggtgatggag gccgcggtga tcggcatacc
     1621 cgaccccaaa cggggggaga gtcccatggc cttcatcatc ccccggcccg ggaagaaggt
     1681 cactgaagaa gagttgatcc atttctgcaa agagaatctg gctaattaca aggtcatcaa
     1741 ggcggtgaag tttgtggaag agtttccccg caacccgaac cggaaggtgc tgaagagaga
     1801 attgcgggag atgctgaaaa gctagttttg ggttgtaggt cccccccacc cctgccctcc
     1861 ccctcgaggg gggagggatg ggagggggtg aggttgtggt ttcagggaac agaggttcat
     1921 ttatggattt ttcactccgc gaagaccagc gaatctttaa agacaccttc cgcaggtttg
     1981 cggaagcgga gatcggcccc ctcgtggaag aggccgagga gacggaaacc tttcccctcc
     2041 gcctttttcc ccagatggga aaactgggat acctgtgtcc ccggtactcg gagaactacg
     2101 ggggagcgga gatcgacaag gtctccgagg ttctcatgcg ggaggagatc tcccgcgtgt
     2161 cccaaggaat tgcctcctcc tggtcggccc actcccatct gggaaccttt cccgtttacc
     2221 attgggggag tgaagaacag aaacaaaaat acctgcttcc cgcgatccgg ggggagaaga
     2281 tcttcgcctt tggcctcacc gagcccaatg ccggcagcga cgtgaaggcc atcgaaacta
     2341 cggccaggcg ggatggagac ggttatgtca tcaacggtcg gaaaatcttc atcaccaatg
     2401 ggaacatctg cgactttctc acgttgatcg cttacaccga caagagccgg ggataccggg
     2461 gaatcagtat ctttctggtg gaaaaaggga cccccggctt catcgtgacc cgcaagctga
     2521 agaaagaagg ggttcgatcc tctgagaccg cggagcttct tttcgaagac tgtcgcattc
     2581 ccgcccggaa cctgatcggc gaagaaggaa gcttctaccg ggtcatggaa acgctcaacg
     2641 aggggcgcat cggcgtagcc ggaaattgcg tgggcatggc ccagggagct tatgaagcgg
     2701 cggtcaagta cgccagggaa agagttcaat tcggtcggcc catcgctcaa tttcaggcca
     2761 tcgcctttaa gctggcggac atggcaacgg agatcgacgc ggccaggctc ctggtccttc
     2821 gagcggcctg gatgatggac cagggaaaga accccatcca ggaggcctcc atggccaagc
     2881 tctttgcctc cgaggtggcg gtgcgggtct ccaaggaggc catgcaggtc cacggaggat
     2941 acggacagat gcgcgagttt ccggtgggcc gcttccatcg ggacgccctg gtctacgtgg
     3001 tgggcgaagg gacttccgaa atccagcgac agataatcgc caagcaaatg gggatggggg
     3061 gttgaaagaa gagagaggat tgacatggtc cgttccaatc agatgctctt cggatacctg
     3121 gccgacaaga acgccaaata ttacccggac aaaatttttc tgattgacga aaaccgcagg
     3181 tgtactttca ggaagtttaa ctcccggacc aacgccctgg cccgggggct cctgaatgcc
     3241 ggaataaaac ctggagaccg ggtggcggtg ctcaaccata attcggtgga gctgttcgaa
     3301 gtctacttcg gggccatgaa agccgggggg attccggttc ccatcaatgt ccgcttggca
     3361 cctcccgaag tcgccgaaat tctgcaggat gccgccccct cccttcttct cgtacaaaag
     3421 aactacctct ctaaaatccc gccgaaaggt ccgggacaga ctttgagccg ggtctacgcc
     3481 attgggggaa gcggaaccct tccggattac gaatccctca tcgggaatta tcccacctcg
     3541 cgggtggaaa ccgcggccga tgacgacgat ccggccatcc tcatctatac cagtggcacg
     3601 acgggaaaac ccaaaggggt catgctgacc caccgcaacc tcctagccga tgtatgggct
     3661 tcctgtgttt cccgccgcct ggcaccggac gatgttgccc tggtaaccgc ccccctctat
     3721 caagccggag cttttggaag catgatatcc aatgtattcc ggggcaacac gatcgtcatc
     3781 ctggatggat tcgacccccc caaggtgctg gccaccatcg agcgggagaa agtcaccacc
     3841 gctctttttg tcccggccat gatcctcaag ctcctggaat ttccggacag cggccggtac
     3901 gacctgagga gcatgcgaac cgtggtctac ggggcggcgc ccatgcccgt aggggccctc
     3961 aagcaggcca tggaacgctt ccgttgggac ttcatgggcg cctgcggggc caccgagacc
     4021 gggccgggct acattgcctt cttggaccgg gaaaaccact acctggatgg atcaccgcag
     4081 atggagaaac gccttcaatc catcggcaaa gaagggatca acgacgaagt acgcatcttt
     4141 gacgacgagg accgggagct tcccccggga gaagtggggg agatcgtcct gcgcgggccc
     4201 aacatcatga aaggatactg gggaaagccc agggagaccg ccgaggcttt acggagcgga
     4261 tggtaccaca cgggagacct gggatatatc gacgcggatg ggtacatcta catcgtggac
     4321 cggaaaaaag acctcatcat cagcgggggt ttcaacgtct atcccaagga gatcgagaat
     4381 gccctggatg cccacctggc ggtgctggag agcgcggtgg tgggaatccc ccatccccgc
     4441 tggggggaga ccccgctggc cttcgtggtc cttaaaaagg gagcgccggc ccccgagctt
     4501 gaagcgttga tgggttttct tcgggagcgg ctggcggatt ataagctgcc caagggggga
     4561 atccagttta cgcaggagtt gccccgcaat gcttcgggaa aggttctcaa gagggagttg
     4621 cgggcccaat atactccgaa gccgcaatga caacatttca aaggatcgag gaatgaaagg
     4681 aacacaaacg gcctcccgcc gcattctgga aggaatacga gtcctggacc tttctacttt
     4741 aataaccggt ccctactgcg ccagcctgct gggggacctg ggaggggaag ttatcaaagt
     4801 ggagcacccc cggggagggg atgggggtcg ccaggtgggg agcaccttcc tggaggggga
     4861 gagcgccctc tttctggcca tcaaccgtaa caagaaagac ctaaccctgg ccctggataa
     4921 gcccgcaggg caggaaattc tgaggcgcct gatcgcgcgc gccgacgtgc tgatcgagaa
     4981 cttccggcct gatatagggc ggcagtatca attggattat gaacaggtcc gggcgatccg
     5041 cccggacatc atttatctct ccatcaccgg tttcggggag gaggggccct accgcctcaa
     5101 gccgggaacg gatcacatct tccagggcct gagcggactg gccaccatct ccggaaaact
     5161 gggtcagggc cccattcgcg tgggaacgcc ggtggtcgat atgaccgctt ccctctattc
     5221 ctgcctcggg gtggtgagcg cccttctcca ccggcagagg accgggcagg ggcagcagat
     5281 atgcgtcaac ctgctcgacg ccgccatgtg cgtgcagagc ccccttttta cggagtactt
     5341 catcaccggg caccgcccca ccccgacggg aaatgccagt cctttcgctt atccagtcga
     5401 tatctttccc agcgccgatg gcaacgtaag catcggggct ttcaatgaca agttctggcg
     5461 gaatttatgc cgcgccttga atctggattc cctggccgat gatccttggt ttaacactcc
     5521 agaaaagaga ctctccaacc gggaggaatt ggggtccctt ctctccaaag tgttggctac
     5581 aaggaagacc cgggagtggc tcaaaatcct ggaggaagcc gacgtccctt gcggcccggt
     5641 tcacgactat gacgccgtct tctccgatcc ccaggtcctt cacaaccagt tggtcaagac
     5701 gcttccccac agccgcctga atgaggtccg cactctgggc aatcccattt ccttcagcca
     5761 aaccccggcc agggaagagc gatcagcgcc gctcctcggg gaagataccg attccattct
     5821 tcactctttg ggatataagc cggaggaagt gaaagagttt cgggagaaaa aaatcatttg
     5881 aggagggaac tcatgaaaaa tggtcgaaat cccttttccc tgctggttgc tctgatcctc
     5941 gttccgattc tgtttcttgc tggaagcagc ctctcggtag ctcaagccca acctgccaag
     6001 accgtttcct ggaagttgca agccgcgtat ccgcccccgg agaagatcat gggtcactgg
     6061 ggggcctacg ggcaggctgc ggaggtcgcc cggcaggtca aggaaaggac ccagggaaag
     6121 ttcgacatca aagtctatcc tcccgatgcc ctgttcaagg ccatggaggc tcctgacgca
     6181 gtgaagcgag gagcggtaga gatgatcctg agcaacgggg cctatcacgt gggcatcctt
     6241 ccggaggcca atctggatta cgggcttccc tacggggtca aaacctccca ggaagccgcc
     6301 aggcttctct ttgaaagcga atattttagg gtgcttcgca aagcctacct ggagaaacat
     6361 caggtcctcc tgctgggtct gaccagcacg agcgcctaca attatctgac ccgttttccc
     6421 atccataaaa tggaggacat gaaagggaag aaaatcagaa cttcgggcgc ctacggcaag
     6481 gtggccctgg cccatggagc cacccctgtc aacctgagcc cagcggagca atacatggcc
     6541 ctgcaaaggg ggaccgtgga cggcacgatc tttcccgcct atgcgggaat tacctacaag
     6601 atgtttgagg tggccaagta ccattcctgg cccccgattt atgccctgat cggggccagc
     6661 ctagtggcca acctgagcgc cttcaacaaa ctccccaagg aataccagca tattctccag
     6721 gaggaagtga ccaaaatggt taaatatact tatgacgttt ctggcccggc cctggagaag
     6781 atcgcccagg aggaaggaag gaagcagttt ggggcggaag ccgtatttat ctccgatgcg
     6841 gaatttttaa aattccgcga ggcggtgaag cccctctggg atgagtggga gaagaagagc
     6901 gattattgcg cccagttggt caagatcgcc aaagactttg cccataaatg attggttgac
     6961 agaaaataag gagggagaga tgaacaggaa aaagatttgc gccgggttca ttgtcctaat
     7021 cgtttccgtt tttttccttt tatgggccgg gaatccacag gccttcgccc aggcgaagcc
     7081 ttccaaggtt tttcaatgga agattcaatc cgcctggcct cccccggaga agttcatggg
     7141 ctactggggc gggtttggtc aggtgtcgga gatcgcccgg caagtcaaag cgcgaaccaa
     7201 cggaggtctg gacatcaagg cattcccgcc ggacgcatta ttcaaagttc tcgaggcccc
     7261 cgatgcggtc aaacggggag cgatcgagat gatctgtagc agcggaccct accacatcaa
     7321 catcctgccg gaagctcttg tcgagtatgg actcccttac ggagtcaaga cctcggaaca
     7381 aatcgccaaa ctcattttgg agaccgaatt tgtctccatc ctccggaaag cttatgccga
     7441 gaagcacaac gtttatatgc taggagtgtg cagcgccagc tcctacaatt acatcacccg
     7501 gttcccggtg cagaagctgg aggacatgaa gggcaaaaaa atccgggcca ccggcacttc
     7561 ggccaggatc gccaccgccc acggtgcgat tcccgtaagc ctctctggag ccgaacaata
     7621 tatggccctg cagagaggaa ccgttgacgg aacgatctac cctccctatg caggcctttc
     7681 ctacaaaatg ttcgaagttg ccaagcaggt atcctggccg ccgatctttg ccgcctccaa
     7741 tatcaactac ctggtcagca tgaacgcctg gcaccagctg ccgaaggaat accaagtgat
     7801 tcttcaagaa gaagtcgcta atatggccaa gtatgcctac catgaatccg gcccgaccct
     7861 ggagaagatt gccagggagg aagggaagaa acagtacggg gcggagtcta cctggctgtc
     7921 cgatactgag tttgccaggt tccagcaggc agtcatgccc atgtgggagg agtgggagaa
     7981 gaagagcccg tattgcgctc agctggtcaa gattgccaaa gagttcgtca aataggtgcg
     8041 ggttaaaagc aatgagactc agggtatccc cctgcctgtc catggaccgg aggttgagga
     8101 aatggaaacg ctggcgagag tgcttgatgg aatcgaccgt ctcaacgaat ggctgggaaa
     8161 gaccttttcc attctggcca tcgttcttac ccttgtggtg gtctatgatg tggtccttcg
     8221 gtatttcttc gcccgtccca ccatctgggg cctggagctg agctgcatcc tcctggccta
     8281 cctcaccttc atgggagggg gctatgcgtt cctccacggg ggccacgtga aagtggattt
     8341 cttctaccag aaatggacgg gccggaccaa ggccacggtg gaccttctca cctacaccct
     8401 gatcattgcc tattgttccg tcctggtctg gcagggaggg gcggtggcct gggaagcgat
     8461 ccaggaggca agggtgagca ccagcgcctg ggctcctccg ctctggccca gccagatcat
     8521 ggttcccatc ggcgcgctgc tggtggggct tcaagcgctg gctcattgga tccgggattt
     8581 tcgcatcgcc tggagcgaaa aaaatgattt ggccagtaag gtatttaccg gtgagggtgg
     8641 tatttttgaa aagaaaaaag agtagtcggt ggaattagga accttcattc tggaatgaaa
     8701 ggagaagtcg gatggatcct gcctggctga ccctgctcct cttcggctcc ctcgtgcttt
     8761 tcctcctcct gggtttgccc atcgccttca cgctcggagg gctggccctg atctttactc
     8821 tctggctctg ggggcccggg ggtctgctta tgttggcctc ccacgcctat ggcgaatcca
     8881 tgaattttat cctggtctcc gccccgctct ttattctcat ggccgtggtc ctcgaaggct
     8941 ccggaatcgc cgaggacctc ttcgagacca tgtacctctg gttcgggggg ctgccggggg
     9001 ggctcctcac ggggacgatc atcatctgcg ccatctttgc cgccatgtcc gggatcagcg
     9061 gggtggccac cgtgaccatg ggcctcatcg ccctgccggc catgctcaaa aggggatacg
     9121 accggaagat gtgcatcggg ggcatcatgt gcggcggagc cctggggatc atcatccctc
     9181 ccagtgtcat cgccattctc tacgggtcga tcaccgggac ctcggtggga aagctcttca
     9241 tgggcgggtt tatccccggg atcatgatcg ccggggtgat gatcttttac accaccgtca
     9301 agtgtgtccg gaaccccaag tatggtcccc ccgtgccggt ggaggaaagg ggtaatttcc
     9361 gggccaagat ggcttccctg aagtttatca tctttcccct tctcctgatc ctcctggtcc
     9421 tgggagtgat ctacagcggg atctgcaccc ccacggaagc ttcggccatc ggcgcctttg
     9481 gggccctcat ctgcagtctg attaaaagaa atctcacctg gaagagcctg aaggcggcca
     9541 ccatccgcac cctgaacatc acctgcatgg ccctgtggat cgtcttcacg gccaagagct
     9601 tctcttcgat ttataccgcc ggaggagctt cggacttcat cctctccctg gtccaaaacc
     9661 tggccatccc tcccctcatg atgatcggga tcatgcagtt catcttcttc atcctgggaa
     9721 tgttcatcga cccggtgggg atgtgcatga tctgctcgcc ggtctttctg cccatcgtca
     9781 ccaacctggg cttcgatcct acctggttcg ggattttgtt catcctcaac tgcggcatgg
     9841 cctatatcac cccgcccttc ggcttcaacc tcttctacat gcgggccatc gccccggcgg
     9901 actgcaccat gggggagatc tatacctcgg tctggccctg ggtcatctgc gaggcgatcg
     9961 ttttgatatt ggtcatgttc ttccccatca tcggcatgta tctgcccaat cagatgggat
    10021 aagaaggagg gagcccgctt tttgatcggg cggatcggtc cggtgcccac cccgtttcgc
    10081 ttcgggcttg cagttccttt cttgttcgct cacccctgcc gatcccttaa gaggttttta
    10141 aggactttcc ccgaaggatt tcgagggagg gagtcgacga actcaacctg
//
LOCUS       DASPRR010000060         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1307940, whole genome shotgun sequence.
ACCESSION   DASPRR010000060 DASPRR010000000
VERSION     DASPRR010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307940"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VEL68_02120"
     CDS             complement(<1..465)
                     /locus_tag="VEL68_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXZ34796.1"
                     /translation="MPGIRRNREKRSSSLPSRGTPESQVLSFFLNLSGEMRRGLSLDL
                     ALRQVVEKAPLLLKADACILYLLDEKENFLQLAQKYNVGRGFSRPLKPREGKVATILR
                     GEKAAVVPNIAKDPSFILHEEAQKEGFISAICAPVRTEKAIGLLVLLRRKGRR"
     gene            706..2460
                     /gene="ispH"
                     /locus_tag="VEL68_02125"
     CDS             706..2460
                     /gene="ispH"
                     /locus_tag="VEL68_02125"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXZ34797.1"
                     /translation="MARVKLAKNAGFCMGVRRAVDMALEEAMKKSGPIYTYGPLIHNP
                     QVLEILEGKGVKTLSGMSVSCPKEGTPVTVIIRAHGVSPGERKELKDSSVRILNATCP
                     HVGKVQGIIQRHAKDGYTTVIVGEKDHAEVIGLLGYANGKGYVVNTLEDLEALPPLER
                     VCLVAQTTQDQKQFQSFAGAIQKRFPEAKVFNTICDSTHRRQDEVLALAKKVEAMVVV
                     GGKQSGNTRRLAKISKEAGIPTFHVETENDLDPQELSRYSVIGVTAGASTPNWLILKV
                     VDRLGRLRLHKGMTYYLENLGRIAAISYIFLALGAGCLAYASVMIQGMQPELSWALVA
                     SFYVFSMHVLNRFTDKASERYNQPGRTEFYERWGSWMIFAGVTSAGVALALAWFQGPL
                     PFLLLLAISGLGMIYNIRIFPGGPGKGVRYQRLKDIPGSKTLFVALAWGTVTSILPAL
                     AAGEWFLPGTAVAFLFSVILVFARSTLYDFKDIQGDLMVGKETIPIVLGRRKAEVLVV
                     SLLIFLGGLLIMAGPLRWAAPVTPWLLFPLVYVLIYYWLYRQQVVGRGFLFEGVVDGS
                     FILAGVLAFFWSLSQRYF"
     gene            2554..3366
                     /locus_tag="VEL68_02130"
     CDS             2554..3366
                     /locus_tag="VEL68_02130"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HXZ34798.1"
                     /translation="MPENDPWMSYQRLYFHPHEIFFQAYWKTFNHFDLQQIRERVRRI
                     KEGDYGLLRSLIQSQDPVPLAEEALRRCQSVLALSPEPQVYLFVGFFSADGATVEVEG
                     LPSIALGMERFKDFRDLSLLVSHEYGHCVQRSLLKEFFPPGGQSLGFKIVAEGLSVLF
                     TRVVYPEIPLHRHLFLTPERLQWCRANQEVLLELAGADLASEKLVPVLFGPGDPNAGL
                     PPRLGYFVAGEMVGHCLAQHGAEEFGKAFPGLENLFRRISPKGIQSSESAGG"
     gene            3376..>4670
                     /locus_tag="VEL68_02135"
     CDS             3376..>4670
                     /locus_tag="VEL68_02135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXZ34799.1"
                     /translation="MGTHKARSAFLLDYEKELNPQQLAVVKAAGGPILVIAGAGSGKT
                     RVVTYRVAYLIESGVDPSGILLVTFTNKAAGEMLHRVQILVPGSMGVGGRVWGGTFHH
                     IGNRILRRHASLVGYQPNFTILDREDAKALMDACIADLKINPKGSRFPKGEVLEDLVG
                     LSVNTRRPIDELVFERYPFFYDLLDDIRAVAEHYRKRKRELNAMDFDDLLFYWETLLR
                     EHREVRARYGGQFQHILVDEYQDTNRIQADIVDLLAEGHGNLMVVGDDSQSIYSFRGA
                     NFANILTFPQRYPKARVFKLEINYRSTPQILHLANTSILNNRVQFPKELQTVRPAGPI
                     PSLVPAQGTMDQAAFVAQKVAEIRDGGIPLSQIAVLYRAHYHSMELQMELTRRGIPYE
                     VRSGLRFFEQAHIKDVSAYLKIVSNPLDELAWKRALRLYP"
BASE COUNT          968 a         1276 c         1359 g         1067 t
ORIGIN      
        1 ccgccggccc ttgcggcgaa gtagcaccag gaggccaatg gctttttcgg ttcgcacggg
       61 tgcgcaaatc gctgagatga acccttcctt ctgggcttcc tcgtgcaata tgaaagaagg
      121 gtctttggcg atattcggca ccaccgccgc cttttcgccg cgcaggatgg tggcgacctt
      181 tccttcgcgt ggtttcaacg gcctggaaaa cccccggccc acgttatatt tttgggccaa
      241 ttgcagaaaa ttttcttttt catcgagaag ataaagaata caggcatcgg ccttcaataa
      301 aagaggagct ttctcaacca cctgccgaag agccagatcc agactgagac ctcgcctcat
      361 ttccccggaa agattcagaa aaaatgaaag tacctgggac tcgggggtgc cccgagaggg
      421 gaggctggag gatctttttt ccctgttcct acggatgccc ggcatttact gggcctcttc
      481 cattatcttg ttgggagtcc gcttattcta cactccggct aacccttgtc aagagcgaaa
      541 tgggttgggg taaggggtca ggcgtattga caccctttca taattccact acactttttc
      601 taggctcttc cggaaattgc gcgggtaagc gatgaattct gataggagca agcctcccct
      661 caaaggatgg gggagaggtc caggaaggat gggtgagttg tcgaaatggc ccgggtgaaa
      721 ctggcgaaaa atgctggctt ttgcatgggg gtccgccggg ccgtggacat ggccctggaa
      781 gaggccatga agaaaagcgg ccccatctat acttatggac cgctgatcca caacccccag
      841 gttcttgaaa tcctcgaggg gaaaggggta aaaacgctga gcggcatgtc ggtttcttgc
      901 ccgaaggaag ggactccggt cacggtgatc atccgcgccc acggagtgag cccgggagag
      961 cggaaggaac tgaaagattc gagtgtgcgc atcctgaatg cgacctgccc gcacgtgggc
     1021 aaggttcagg ggatcataca gcggcacgcc aaagatgggt atacgacggt gatcgtcggc
     1081 gagaaggatc atgctgaggt catcggtttg ctgggatacg cgaacgggaa gggatacgtc
     1141 gtcaataccc tggaggacct ggaagcactt cccccgctgg agagggtctg cctggttgcc
     1201 cagaccacgc aggaccagaa gcaattccag tcctttgccg gcgcgattca gaagagattc
     1261 ccggaggcga aggtattcaa caccatctgc gactccaccc accgccgcca ggatgaggtg
     1321 ttggccctgg ccaagaaggt ggaggccatg gtggtggtgg ggggaaagca gagcgggaat
     1381 acccgccgcc tggcgaagat ttcgaaagaa gcagggattc caaccttcca tgtggaaacg
     1441 gaaaatgacc tggacccgca agagctctcc cggtattccg tcataggcgt cacagccggc
     1501 gcctccacgc ccaactggct gatcctgaaa gtggtggatc ggctggggcg cctacggctg
     1561 cataaaggga tgacttatta cttggaaaac ctgggcagga ttgcggccat cagttacatc
     1621 tttttagctt tgggagcggg ctgtttagcc tatgccagcg ttatgatcca gggaatgcag
     1681 cccgagcttt cctgggccct ggttgcttcc ttctacgttt tttccatgca tgtgctgaat
     1741 cgttttaccg ataaggcctc ggagaggtac aaccagcccg gccgaacgga gttctacgag
     1801 aggtggggct catggatgat cttcgctggg gtcacttcgg caggcgtggc cttggcccta
     1861 gcctggttcc agggtccttt gcccttcctt ctccttctgg ccatctcagg actggggatg
     1921 atttataata ttcgaatttt tcccggaggt cccggaaagg gggtccgtta ccagaggctc
     1981 aaggacatcc ctgggtcgaa gacccttttc gtcgctctcg cctggggtac ggtgacctcc
     2041 atcctccccg cactggctgc cggggagtgg tttctacccg ggacggcggt cgcctttctc
     2101 ttcagcgtaa ttctggtttt tgcccggtca acgctctatg acttcaaaga catccaggga
     2161 gacctgatgg tcggcaagga aaccattccc attgttctgg gaaggcggaa ggctgaggtt
     2221 ctggtcgttt ccctcctcat tttcctgggg ggcctgctga tcatggccgg acccctgagg
     2281 tgggcggctc cggttacccc ctggctcctt tttcctctgg tgtatgtact catttattac
     2341 tggctttacc gccagcaggt cgtgggcaga gggttcctgt tcgaaggggt ggtcgatgga
     2401 agttttatcc ttgccggcgt tttagctttc ttctggtccc tgtctcagag atatttttag
     2461 aattttcacc ggcataactt tagcgcactt cggcaattca atttgatgaa tgtcaaacct
     2521 ctgtacctcg atttcttcag gttcctggat caaatgccag agaacgaccc ctggatgagc
     2581 taccagcgac tttacttcca cccccacgaa attttttttc aggcctactg gaaaactttc
     2641 aatcactttg acctgcaaca gattcgggag cgggtcaggc ggatcaaaga gggagactac
     2701 ggccttctta gatcgttgat ccaatctcag gaccccgtgc ccttagcgga agaggcgctg
     2761 cggagatgcc agtcggttct cgccctgagt ccagaacccc aggtctatct attcgttggt
     2821 ttcttcagcg ccgatggcgc gaccgtagaa gtcgaaggct taccgtccat cgctctgggg
     2881 atggagcggt tcaaggactt cagggacctc tctctcctgg tgtcccacga gtacggccac
     2941 tgcgtccaac ggtcactcct gaaagaattt tttccccccg gaggacaaag cctgggtttt
     3001 aaaatcgtgg cggagggcct gtctgtcctt ttcaccaggg tcgtctaccc ggagattccc
     3061 ctgcaccgac acctctttct gaccccggaa cggctgcaat ggtgtcgagc aaaccaggag
     3121 gtgctgctcg aattggccgg ggcggatttg gcttccgaga aactcgtgcc ggtccttttc
     3181 gggccgggag atccaaacgc tggacttcct ccccgcctgg ggtatttcgt ggccggcgag
     3241 atggtgggac attgccttgc ccagcacgga gcggaggaat tcggcaaagc ttttcccggg
     3301 cttgaaaatt tgtttcgcag gatttcgcca aaaggaattc aaagctccga atcggcggga
     3361 ggatgaaagg gcacggtggg tacgcataaa gcccggtccg cttttctgct ggactatgaa
     3421 aaggagttaa acccccagca gttggccgta gttaaagcgg ccgggggacc catactggtc
     3481 attgccggag cgggaagcgg gaaaacccgg gtcgtaacgt accgggtggc ctatttgatc
     3541 gaatccgggg ttgacccttc tggcatattg ttggttactt tcacgaataa ggccgccggg
     3601 gagatgctcc accgggttca gatcctcgtt ccggggtcga tgggcgtggg tggaagagtg
     3661 tgggggggga catttcatca tatcggaaac cgcatcctcc ggcggcatgc ttccctggtg
     3721 gggtatcagc ccaactttac gattctcgac cgggaagacg ccaaagccct catggatgcc
     3781 tgcatcgccg acctgaagat caaccccaaa ggatcgcggt ttcccaaggg agaagtcctg
     3841 gaggacctgg tcgggctgtc cgtgaatacc cgtcggccca tcgatgagct ggtcttcgaa
     3901 cgttatcctt ttttctacga tctcttggac gacatccggg ctgtagccga gcactaccgc
     3961 aagaggaagc gggaactgaa tgccatggat tttgacgacc tgctcttcta ctgggaaact
     4021 cttctgcggg agcaccggga agtgcgggcg cgctacggcg gccagtttca gcatattctg
     4081 gtggatgaat accaggacac caatcgcatc caggccgata tcgtggacct cctggcggag
     4141 ggacacggga acctcatggt agtgggcgat gactcgcaat cgatctattc cttccgggga
     4201 gcgaacttcg ccaacatcct gacttttccc cagcgctatc cgaaggccag ggtcttcaag
     4261 ctggagatca attaccggtc cacgccgcag atcctgcatc tggccaacac cagcatcctg
     4321 aacaaccggg tccaattccc caaagagttg cagaccgtcc gaccggcggg gcccatccct
     4381 tcgctcgttc ccgctcaggg caccatggac caggcggctt ttgttgccca aaaagtggcg
     4441 gaaatccgcg acggaggaat tcccctctcc cagatcgccg ttctttatcg ggctcactat
     4501 cattccatgg aattgcagat ggagttgacc cggcggggaa tcccctacga ggtccggtcg
     4561 ggcctgcgct tcttcgaaca ggcccatatc aaggatgtga gcgcctactt gaaaatcgtc
     4621 agcaatcctc tggacgaact ggcctggaaa cgggccctgc ggctttatcc
//
LOCUS       DASPRR010000061         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_666706, whole genome shotgun sequence.
ACCESSION   DASPRR010000061 DASPRR010000000
VERSION     DASPRR010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666706"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..760)
                     /locus_tag="VEL68_02140"
     CDS             complement(26..760)
                     /locus_tag="VEL68_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ34800.1"
                     /translation="MKIDLEKCLGCQKCADYCPVDAISYIPDEKKPKKKKAVIDLEEC
                     VECGVCFRAGVCKPEAIFRQEIGWPRSVRLAFSDPMIEHKNTGVPGRGTEEMKTNEVT
                     GRFKRGTYGMAVEVGRPGVAGRLEDLEKIAISLAKIGVEFEPRNPVTFLFEDVKTGKL
                     KPEVRKEKVLSAIIEFVVKPEQIRPVLQALKDISPKIDSVFSLDLISFPEESEKAPAL
                     KIVKEMGFPVYPNGKVNLGLGKVLNK"
     gene            complement(881..1198)
                     /locus_tag="VEL68_02145"
     CDS             complement(881..1198)
                     /locus_tag="VEL68_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34801.1"
                     /translation="MRIRIELWMWLGRDPGGGFQALSEMRSFREMDVEEELTIIQLLD
                     RLAVKSRVIEEKIIDRKNRKLLPNLSVVVTRDGLVISPFDVEKTQIRDGYKITILPLY
                     VGG"
     gene            complement(1372..>2262)
                     /locus_tag="VEL68_02150"
     CDS             complement(1372..>2262)
                     /locus_tag="VEL68_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ34802.1"
                     /translation="AWGPQIGNTELGAVVMLTKEVDKLGMDCNEGSWVVGWAMECYGK
                     GVFTKKETDGLDLSWGNVEAVKELLNRISRRQGYIGNLLADGVMRASKKVGGPAADWA
                     IYGCKGSAPRGHDHRGTTRWYELLDTCVTNTSSLDATWGGVHPKLVDQEEATDHFSHE
                     QVSTINAKFSGIRLFDDCLGTCRFASPHPKLQLECFNAVTGWNWKLEDGFAVGRRISN
                     LLRVFNFRHGAKIEDERPSKRYGSIPVDGPAKGKNIMEKWPQMVENYYRLVGWDPKTG
                     KPLPGTLEKLGLKEMIKDLK"
BASE COUNT          440 a          701 c          554 g          567 t
ORIGIN      
        1 aaccacaggg ggttgcccct acaatctatt tattaagcac tttccccagt cccaggttca
       61 ccttcccgtt gggatagacc gggaagccca tctctttgac tatcttcagg gccggggctt
      121 tctccgactc ctcggggaag ctgatcaggt ccagggagaa gacgctgtcg attttcgggg
      181 aaatatcttt caaggcctga aggaccggcc tgatctgctc gggcttgacc acgaactcga
      241 tgatcgccga aagaaccttt tccttacgga cctcgggctt cagctttccg gtctttacat
      301 cctcgaagag gaaggtcacc ggattcctcg gttcgaattc caccccgatt ttggccaagg
      361 aaatggcgat tttttcgagg tcttccagcc ttccggccac acccgggcgg cccacttcaa
      421 cggccattcc gtatgtgccc ctcttgaacc gtcccgtgac ctcattggtc ttcatctcct
      481 ccgtgccccg tcccgggacg ccggtgttct tgtgctcgat catgggatcg ctgaaagcca
      541 ggcggacact ccgcggccag ccgatttcct gccggaaaat ggcctcgggc ttgcagaccc
      601 cggcccggaa gcacacgccg cattccacgc actcttccag gtcgatgacc gcctttttct
      661 tcttgggctt cttttcatcc ggaatgtagg aaatggcgtc taccggacaa tagtcggcgc
      721 acttctgaca tccaaggcat ttctccaaat caatcttcat ttcttacctc tcttttttgt
      781 tgtcaatact cgctcctcta ttatccacca gccgtaggga cgattctcgt agagtcgagt
      841 ctgcgacatg aatcgcttct acgattctta agacaatctt tcaccctccc acatagaggg
      901 gcaggatcgt tattttgtag ccatccctga tttgagtctt ttccacatcg aacgggctga
      961 tcaccaatcc atcgcgggtc acgaccacgc tgaggttggg aagcagtttc cggtttttcc
     1021 ggtcgataat cttttcttcg atcacccggg atttaacagc caggcggtcc aggagctgga
     1081 tgatggtgag ctcctcctcc acatccattt cccggaagga ccgcatttcc gagagggcct
     1141 gaaagccgcc tccggggtcc cttcccagcc acatccacag ttcaattcga attcgcatgg
     1201 atcacaccca aaattccaaa tttcaagcac ccaactccag ataaattcca aaaaccaaat
     1261 atccaaacgg gtaagagcga ctaaccggcc catggcagtt ttgtttcagt cattcgaatt
     1321 tgaaaactgg tgcttatttg gaattgtgat ttggtgcttg ggatttcttc tttatttcag
     1381 gtctttaatc atctctttca gcccgagctt ttccagcgtc cccggcaggg gctttccggt
     1441 cttcgggtcc cagcccacca gacggtaata gttctccacc atctgcggcc atttctccat
     1501 aatgtttttg cccttagccg ggccgtccac gggaatggaa ccataccgtt tggatggcct
     1561 ttcgtcctcg atcttcgccc catggcgaaa gttgaagacc cgcagcaggt ttgagatccg
     1621 ccttcccact gcgaacccgt cttccagctt ccagttccat ccggtgaccg cattgaagca
     1681 ttccagctgg agcttggggt ggggcgaggc aaaccggcag gtgcccaggc aatcgtcaaa
     1741 gagccggatc ccgctgaact tggcgttgat ggtggagacc tgttcgtggg agaaatgatc
     1801 cgtcgcctcc tcctggtcca ccaatttagg atgcactcct ccccaggtgg cgtccagaga
     1861 gctggtgttg gtcacgcagg tgtccaggag ttcataccag cgggtcgtcc cgcggtggtc
     1921 atggccccgg ggggcggacc ccttgcaccc gtaaatggcc cagtccgcag ccggcccgcc
     1981 aaccttcttg gaggcacgca tgaccccgtc ggccagaaga tttccgatgt atccctgccg
     2041 ccgggagatg cggttcagga gttcctttac cgcttccaca tttccccagc ttaggtctag
     2101 gccatcggtc tccttcttgg tgaaaacgcc tttcccatag cattccatgg cccagcccac
     2161 gacccaggac ccctcgttgc aatccattcc cagcttgtcc acttccttgg tcagcatgac
     2221 cacggcgccc agttcggtat tgccgatctg tgggccccag gc
//
LOCUS       DASPRR010000062         7316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2113367, whole genome shotgun sequence.
ACCESSION   DASPRR010000062 DASPRR010000000
VERSION     DASPRR010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113367"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VEL68_02155"
     CDS             complement(<1..1185)
                     /locus_tag="VEL68_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXZ34803.1"
                     /translation="MVFKHDVVIVGSGLAGLRAAMEVAGTIDVALISKVYPTRSHSGA
                     AQGGIAAALGNEEPDSWEWHMYDTVKGGDYLTDQDAAEILAQDAPRAIYELEHMGVPF
                     NRTPDGKIAQRPFGGHTRDFGQAAIKRACHAADRTGRVILDTLYWETVRKGIKVYNEF
                     QMVDLILKGRQAMGIVVYELATGQVHLFHSRMVVLATGGFGKVYRTTSNCFANSGDGV
                     YLAYRAGVPLEDMEFVQFHPTGVYGPGILITEAARGEGGILRNGKGERFMDRYAPVIK
                     DLAPRDMVSRAIYREIAEGRGVDGKDCVYLDLTHLGREKINEKLSDISSFAKIYLGID
                     AAVQPIPVSPTCHYMMGGIPTNLEGQVLDQDQRVVPGLYAIGECACLSLHGANRLGCN
                     SLL"
     gene            1362..1610
                     /locus_tag="VEL68_02160"
     CDS             1362..1610
                     /locus_tag="VEL68_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34804.1"
                     /translation="MAGKVPPLARKPNPTTGPIRRKMAVESTGVRITKAVYQKEAFAY
                     HQTIMDFPLSISTEAIDAEKFKIQFKKSIYIRKMNFGA"
     gene            1791..2870
                     /locus_tag="VEL68_02165"
     CDS             1791..2870
                     /locus_tag="VEL68_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXZ34805.1"
                     /translation="MTLTGVLPKRFSCLFILVFFILAAPAVWADTSHIVKKGESLSSI
                     AQKYRISISRIQEANDLEDTKLQIGQSLTIPLSASTISNSSSSSPKTRHQNTSPEREI
                     PDFHLVKNGDTLSGIAKRYHLTINDLQEINELKGTRLRIGQVLQLKQEEEVSEEEKIQ
                     SGEGSYESALASNNGSAIPISQGADIVEERDLNPLVTVAESFLGMKYRRGGTNVKTGL
                     DCSAYVQKVFRMVGMDLPRTAREQFGVGMEVARDALRLGDLVFFKRSQTKRPAHVGIY
                     IGNEQFIHSSTTKRKIRVDSLNTRYFSARFIGGRRIQEIKVQPDIPKPEMDDSHFSFL
                     AFEPTLSFPLLSATAQPSPSYDPDL"
     gene            3050..4177
                     /locus_tag="VEL68_02170"
     CDS             3050..4177
                     /locus_tag="VEL68_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016357944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ34806.1"
                     /translation="METLVIHGNEACARGAIAAGCRFFAGYPITPASEITEVMSRLLP
                     RTRGVFLQMEDEIASICAILGAAWGGAKTCTATSGPGFTLMQEGIGWAAETDTPVVVI
                     DVMRGGPATGQPTSSSQQDVMQAKYGSHGDYEVIVLVPSSAQEAFDLTVKAFNLAEVF
                     RNPVILLTDEIVGHTREKVRIPEKVEIVERRKPATNPADYVPFRSAAKGLLDGMPAFN
                     QGYGILVDGQLHDEFGGRAGHKPEVSAAMVERICRKITDHADELADVEEWMLEDAEIA
                     FIAFGSPARPALRAAKDARTQGIKAGFLKLRVLWPFPDKIIARVARQARRVIVPEMNM
                     GKVFREVKRVVAGNAEVISLPKLGGEVHTPAEILRSMFSDQ"
     gene            4286..5023
                     /locus_tag="VEL68_02175"
     CDS             4286..5023
                     /locus_tag="VEL68_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ34807.1"
                     /translation="MNVFLRAVDDLGIFKELALVGGIGCSGWLPTYLQSDILHVLHGR
                     AIPFACGLKLTDPRRKVVVFTGDGDCVGIGGNHFIHAARRNIDLTVIMINNGIYGMTG
                     GQVAPTTPLQGRTQTSPYGNLEPPFDVCDLARSAGATYVARWTAAHPVQLLKTIKEGI
                     EHPGFTFIEVMAQCPTQAGRYIEGTSNAGELLNLLKKRSVSQEKAKETPPEEMTGKFV
                     VGKLHHLTGKKEFSQAIYESFLPEKSR"
     gene            4992..5231
                     /locus_tag="VEL68_02180"
     CDS             4992..5231
                     /locus_tag="VEL68_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004589866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXZ34808.1"
                     /translation="MKASSRKRVAKEIVIDDQFCKGCNLCIEVCPRKVFAKSGKRNRS
                     GYSMPQAADMGKCSVCFLCEMTCPDLAITIIEENP"
     gene            5228..5779
                     /locus_tag="VEL68_02185"
     CDS             5228..5779
                     /locus_tag="VEL68_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ34809.1"
                     /translation="MRREVRIAGFGGQGVVTLGTILAVAAGVHEDLEVAQSQSYGPEA
                     RGGACRSDVVISDEPIDYTKPLALDVFVALSPAAAEKYWSEVREKRTLVMVDKTLVPS
                     TPDHSPPVLGIEATRIAEEELKDRVAANMIMLGAMAAVTGWVSLKALEETLSGRMSAK
                     ALDTSKEALRRGFELGKALKAKD"
     gene            5987..7183
                     /locus_tag="VEL68_02190"
     CDS             5987..7183
                     /locus_tag="VEL68_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HXZ34810.1"
                     /translation="MIDNTLFKKIHGCNVGGYVGAALGEPSRRAGGGVFDCYKGLCGP
                     IEGAHYKVIDELLGKPVDKMLPQVKAWVGGEVPKIGYEPQAWRTIRWHNGPLFKVPAL
                     NYPPGTNEDGGERKWLVMKAIWDRGGRITKEDLRASWLKYVNPEWFGFHLLPRDKVTY
                     ENMKKYPASEVGRYDRWPGNVDCLMMIHPIGIINACDPQLAAMDALDVCQTIQSSLIS
                     FAPDSAAAIAAAIAEAFNPQATVDSIIKAGKAYVNENIGQVIDECIGYVKQVPDMLEV
                     RELYWKRFGGRVPTDSLEVVGESFAMFWLTKGDPKQCMIAGSSLGRDTDCIASIAGAI
                     AGAFKGIDAMPKEWVDICQKAMSADPHEIITMSMEDQSKALYDMVLKIMGERKKQVEI
                     IESLKK"
BASE COUNT         1753 a         1934 c         2097 g         1532 t
ORIGIN      
        1 gagaagggaa ttgcatccga ggcggttggc cccgtgcaga gataaacagg cgcattcccc
       61 gatggcgtac aaacccggaa cgacccgctg atcttgatcc aaaacctggc cctccaggtt
      121 ggtgggaatc ccgcccatca tgtaatggca agtgggggag acgggaatcg gttgaacggc
      181 ggcatcgatg cccaggtaga tcttggcaaa ggagcttata tccgatagtt tttcgttgat
      241 tttttctcgg cccagatggg tcaggtcgag gtagacacag tctttgccgt cgacgcccct
      301 cccttcggcg atttcccggt agatggctcg gctcaccata tcccgggggg caaggtcctt
      361 aatgaccggg gcgtatctat ccatgaagcg ctcgcccttg ccattccgca gaatgccccc
      421 ttccccccgc gctgcttcgg tgatgagaat cccagggcca tagactccgg tgggatggaa
      481 ctggacgaat tccatatcct ccaggggaac gcccgctcgg taagccaggt aaaccccatc
      541 tccgctattg gcaaagcagt ttgatgttgt ccggtaaacc ttcccgaatc ctccggtggc
      601 cagtacgacc atcctgctgt ggaagagatg gacctgccca gtggccaatt cgtagaccac
      661 tatacccatg gcttgacggc ccttcaggat cagatcgacc atctgaaact cgttatagac
      721 tttgatcccc ttccgaaccg tctcccaata aagggtgtcc aggatgactc tgccggtccg
      781 gtcggctgca tgacaggccc tcttaattgc cgcctgcccg aagtcgcggg tatggccccc
      841 gaaaggccgc tgggcgatct tcccatccgg ggtgcggtta aagggaaccc ccatgtgctc
      901 aagctcatag atcgctcggg gagcatcctg ggccagaatc tcggccgcat cttggtcggt
      961 gagataatcc cctcctttga ccgtatcgta catgtgccat tcccaggagt ccggttcttc
     1021 gtttcccaaa gcggcggcaa ttcccccctg ggccgcgccg gaatgggacc gagtgggata
     1081 aaccttgctg atcaaggcta catcaatcgt tccagcgact tccatggccg cccttagccc
     1141 ggcgaggccg gaccctacga tcaccacatc atgcttgaag accatcgttc tccccctgcc
     1201 ttggggtttg aatacgccac tgaaagggga aataccaacg ggaagaaggg ttcaccatgg
     1261 ttttatgcta acaggaagct cgaatcggat catatagaaa aaaataccca ttttcggtag
     1321 gaatagccag tatttttgaa aagaaatcat tgcgaggaaa tatggccgga aaagtgcctc
     1381 ccctggctag aaaacccaac cccactactg gacccattag gagaaagatg gccgtcgaaa
     1441 gtacaggggt ccggataaca aaagcggttt atcaaaagga agcttttgcc tatcaccaga
     1501 caataatgga ctttccatta tcgatttcga cagaagcaat agatgctgag aaattcaaaa
     1561 tccaattcaa aaagtcgatt tatatcagga agatgaattt cggtgcctga ccagcttcct
     1621 ttaaagggca aaggatttgc atggacttga ggtgtttgac ctgcatcttc ctcacgctcg
     1681 gcctgatgag ccaaaaaatg actgggaaag gttgaaaaat ttagtgatct tttttataat
     1741 tgggccgatt tagaaaaata gccatgcctt gctggatgaa aaaaacaaca atgactctga
     1801 ccggagttct ccctaagcga ttttcttgcc ttttcatttt ggtctttttc atcctagccg
     1861 ccccggcagt gtgggcggac acgagccaca tcgtgaagaa gggtgagtct ctctcctcta
     1921 tcgcccaaaa gtaccggatc tccatttcca ggattcaaga ggccaacgac ctggaagata
     1981 cgaagctaca gatcggccaa agcttgacca ttcctctatc ggcctcgacc atttcgaact
     2041 catcttcctc ctctccaaag acaagacacc agaatacgtc cccggagcgg gaaattccgg
     2101 atttccattt agttaagaat ggagacaccc tgtccgggat cgccaagcgt taccatctta
     2161 cgatcaatga cctgcaagag atcaatgaac tgaagggaac ccggctgcgg attggtcaag
     2221 ttttgcagct gaagcaggag gaagaggttt ccgaggaaga gaaaattcaa agcggggagg
     2281 ggagctacga atcggctttg gcctcgaata acggctcggc gattcccatt tcgcaagggg
     2341 cggatatagt ggaagaacgg gatttgaacc ccctggttac ggtggcggaa agtttcctgg
     2401 ggatgaagta ccgccggggt gggaccaatg tgaaaaccgg gctcgattgt tccgcctacg
     2461 ttcagaaggt attccggatg gtaggcatgg accttccccg gaccgccagg gagcagttcg
     2521 gggtgggtat ggaagtggcc cgtgacgcgc tccgattggg ggatctcgtg ttcttcaaac
     2581 gcagccaaac gaagcgtccg gctcatgtgg gaatttacat aggcaatgaa cagttcattc
     2641 actcttccac gaccaaacgg aaaatcaggg tggacagttt gaacacgcgg tatttttccg
     2701 caaggtttat tggggggaga cggattcagg aaatcaaggt gcagccggat atccccaaac
     2761 ccgagatgga cgattctcat ttctcttttc tcgccttcga gccaacgttg agctttcccc
     2821 ttctttccgc aactgcccag ccgagccctt cctatgatcc tgatctatga gccttcggcc
     2881 ggcattgaat tttagagcct tccttgttat agtgaaaccg ctttcccagc agttcatcgc
     2941 tatcgaatat taggcgtgaa tagtcttcat ccgaaaccca tctcccctct cgaaggagag
     3001 cggaaaatcg ggtttccgga tgagggctgg gtaggaagaa ggattttcag tggaaacctt
     3061 ggtcattcat ggtaatgaag cctgcgcccg gggcgcgatt gccgcgggct gccggttctt
     3121 tgccgggtat cccatcactc cggcttccga gattacggaa gtcatgtccc gcctccttcc
     3181 tcgaacccgg ggggttttcc tgcagatgga ggacgagatc gcttccatct gcgccatcct
     3241 cggggctgct tggggaggag cgaaaacctg caccgccact tcgggcccgg gattcaccct
     3301 catgcaggaa ggaatcgggt gggcagcgga gaccgatacc ccggtcgtgg tcatcgatgt
     3361 catgagggga ggcccggcaa ccggacagcc gacctcttcc tcccagcagg atgtgatgca
     3421 ggcaaaatac gggtcccatg gggattacga agtgattgtc ctggttccct ccagcgccca
     3481 ggaggccttt gacctgacag tgaaggcctt caacctggcc gaagtcttcc gaaacccagt
     3541 catcctcctc accgatgaaa tcgtgggcca cacccgggag aaagtccgaa tcccggaaaa
     3601 agtggagatc gtagagagga gaaagccggc gaccaatccc gcggactacg tgcccttccg
     3661 atcggcggcc aaagggcttc tcgacgggat gccggctttc aaccagggtt acggaattct
     3721 ggtggacggg cagctgcacg acgaatttgg gggacgggca gggcacaagc ccgaggtctc
     3781 cgccgctatg gtggaacgta tctgccgcaa gatcacggac catgccgacg agttggcgga
     3841 tgtggaggag tggatgctgg aggacgcgga aatcgccttc attgccttcg gcagtccggc
     3901 caggccagcc ttgagggcgg ccaaagatgc ccgcacccag ggaatcaaag ccggtttcct
     3961 gaaactccgg gtcctttggc ccttcccgga caaaatcatt gcgcgggtgg caaggcaggc
     4021 cagacgggtt atcgtcccgg agatgaacat gggaaaggta ttccgggaag tgaaaagagt
     4081 ggtcgctggc aatgcggaag tcatttccct ccctaaattg ggaggagaag ttcacactcc
     4141 cgcggaaatc ctaaggtcaa tgttcagtga tcaatgacca ttgaccattg ccaattgttt
     4201 ttttatgcat cccttagcta acaagtatat tcggaaaaga acgctccctt tgatcttctg
     4261 ccctggctgc ggggatggga ctgttttgaa tgttttcttg cgggcggtgg atgacctggg
     4321 aatcttcaag gagctggccc tagtcggggg cattgggtgt tccggctggc tccccaccta
     4381 tctccagtcg gacatccttc acgttctcca cggaagggcc attcccttcg cctgcgggtt
     4441 gaaactcacc gatcctcgcc gaaaagtggt tgtctttact ggagacgggg actgcgtggg
     4501 cattggggga aaccatttca ttcatgccgc ccgaagaaac atcgacctca cggtgatcat
     4561 gatcaacaac gggatctacg ggatgaccgg cggccaggtg gcccccacga ccccgctcca
     4621 ggggcggacc cagacctcgc cctacggaaa cctggaaccc cccttcgacg tttgcgatct
     4681 ggcccgttcc gccggagcca catatgtggc gcgctggacg gcagcccatc cggttcaact
     4741 cctcaaaacc atcaaagaag ggatcgagca tccgggattc actttcatcg aggtcatggc
     4801 tcaatgtccg acccaggcgg ggcgctatat cgagggaacc tcgaacgcgg gggaactgct
     4861 gaaccttctg aagaaaaggt ccgtttccca agaaaaggcc aaggagactc cgcccgaaga
     4921 aatgaccggg aaattcgtgg tcggaaaact gcaccatctg accggtaaga aagaattcag
     4981 ccaggcgatc tatgaaagct tcctcccgga aaagagtcgc taaagaaatt gtcatcgacg
     5041 atcaattctg caaagggtgc aatctctgca tcgaggtatg cccgcgaaag gtctttgcca
     5101 aaagcggcaa gcgcaaccgc tccggctatt ccatgcccca ggccgcggac atgggaaaat
     5161 gctcggtctg ctttctctgc gaaatgacct gcccggattt ggctatcacc ataatcgagg
     5221 aaaacccgtg agaagagaag tgcggattgc cggatttgga ggccaggggg tcgttaccct
     5281 gggaacgatc ctggcggtgg cggccggggt ccatgaagac ctggaagtgg cccaatccca
     5341 aagctacggt cccgaagccc ggggaggagc ctgccgatcc gatgtggtca tcagcgatga
     5401 acccatcgat tacaccaaac cgctggccct ggatgtgttt gtagctctgt cccccgccgc
     5461 agcggaaaaa tattggtcgg aggtccggga aaagagaacc ctggttatgg tggataagac
     5521 cctggtgccc agtacccccg atcactcgcc gccggtgcta ggcattgagg ctacccgaat
     5581 tgctgaggaa gagctaaagg accgggtggc cgccaatatg atcatgctgg gagcgatggc
     5641 cgcggtaacc ggctgggtca gcctgaaggc cctggaagag accttaagcg ggcggatgtc
     5701 tgccaaggca ctggatacca gtaaggaggc tttgaggcgg gggttcgagc tgggaaaggc
     5761 attgaaggca aaagactgaa ccacagagaa gccagagatt agaaagaaat cccaaacttc
     5821 aaatcccaaa tcccaaataa attccaaatt gcagaaaagc ggaagagtgg gaagctggga
     5881 tttggtattt tgggagttgg tgcttatttg gaatttggtg cttggaattt gaaatctgcc
     5941 tccatgctgt gtgatcaggg atgaaaattg agaaaaggag tgagttatga tcgacaacac
     6001 gctgttcaaa aagattcacg ggtgcaacgt gggcggatac gtgggcgccg ccttggggga
     6061 gccgagccga cgggccggcg gcggcgtctt tgactgctac aagggcctgt gcggacctat
     6121 cgagggggcc cattataaag tcatcgacga actgctggga aaaccggtgg ataagatgct
     6181 tccccaggtt aaagcgtggg tgggcgggga ggtgcccaag atcggctatg aaccccaggc
     6241 ctggaggacg atccgctggc ataacgggcc cctgttcaaa gtcccggcct tgaattatcc
     6301 tcccggaacc aacgaagatg gcggggagag aaagtggctg gtcatgaagg ccatctggga
     6361 ccggggaggg aggatcacca aggaggacct ccgggcttcc tggttgaaat acgtaaatcc
     6421 cgaatggttt ggattccacc tgctgccgcg ggacaaagtc acttatgaga acatgaagaa
     6481 atatccggcc agtgaagtgg gaagatatga ccgctggccg ggtaacgtgg actgcctcat
     6541 gatgattcat cccatcggga tcattaacgc ctgcgacccc cagctggcgg ccatggacgc
     6601 cctggatgtt tgccagacga ttcaatcctc cctgatcagc ttcgctcccg attccgcagc
     6661 agccatcgct gcggccattg ccgaggcctt caacccccag gccacggtcg attccatcat
     6721 caaggcggga aaagcctacg tgaatgaaaa tatcggccag gtcatcgatg agtgcatcgg
     6781 atacgtcaaa caagtcccgg acatgttgga agtccgggag ctctactgga agaggttcgg
     6841 cgggagggta cccacggact ccctggaagt agtgggcgag tccttcgcca tgttctggct
     6901 taccaagggc gatcccaaac agtgcatgat cgctgggtct tccctgggcc gagatacgga
     6961 ctgcatcgcc agcatcgccg gggctatcgc cggagcgttc aagggaatcg atgctatgcc
     7021 caaggagtgg gtggacattt gccagaaggc catgagtgca gacccccacg agatcatcac
     7081 catgagcatg gaagatcagt ccaaagccct ctacgacatg gtcctgaaga tcatggggga
     7141 gaggaagaag caggtcgaga taatcgagtc tttgaaaaaa tagtttggag gaacacctac
     7201 ggagcgtggg aaagttcaag ggaccagcca tcctttcagg ctttccccct ggaggccgga
     7261 ttctctgagg gaggatcgga aaggagagaa aaaatgaaaa agggctttcc tatttt
//
LOCUS       DASPRR010000063        15890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_666882, whole genome shotgun sequence.
ACCESSION   DASPRR010000063 DASPRR010000000
VERSION     DASPRR010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15890)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15890
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666882"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VEL68_02195"
     CDS             <1..636
                     /locus_tag="VEL68_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXZ34811.1"
                     /translation="LIMLHDESLGSLTTSKQRQAVGTMLRNVNALINMIQKYLQLGRI
                     ESGRMEVNKVQMPIFNEALAPVLEDEKQQLDARRMQVVLENEEAFRRVEEEADPVLLR
                     IVFDNLIGNAIKYGKEGGTIWAGFREEPTELCFYVKNEGRGIPADKLNVVFEKFARLE
                     GELERRRRGTGLGLFNAKQIVEKHGGKIWAESEEGKWANFLFTLPKGVKGS"
     gene            680..1069
                     /locus_tag="VEL68_02200"
     CDS             680..1069
                     /locus_tag="VEL68_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ34812.1"
                     /translation="MEGRRKVLVIDDEPDLVEMIRMALERMFEVITAFNGKEGIAKAR
                     SERPDVIVLDVMMPEKDGFTACRELKEDPQTASIPVLILTGVAEHFGRTKFHRMGGME
                     IEAEDFIPKPVDPNELLRRVAALLKKG"
     gene            1151..1849
                     /locus_tag="VEL68_02205"
     CDS             1151..1849
                     /locus_tag="VEL68_02205"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ34813.1"
                     /translation="MEFEKAVLGEEEMIELEPLKSEEIARNLDSQAEVFFIGCSGWKE
                     VCNPGGEAQLQLLKKDLSLRGVSFTGTLVVDALCNKGMDELSLLTQIRQACRSRTLLV
                     LSCEVGVRALGAVAGQRVMPALRTLAAEGFQGVLGDRELCRLCGECLLEASGGLCPLY
                     FCPKEMLTGPCQGARQGQCEVDPKTPCGWELIYDRLKTQGRLEMLKTSAEPRDHSKIL
                     PLLRLRSTLAAGRK"
     gene            1986..2861
                     /locus_tag="VEL68_02210"
     CDS             1986..2861
                     /locus_tag="VEL68_02210"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXZ34814.1"
                     /translation="MTFQERLQENKFSITCEINPPKGAEAAERLEEAETLKGRVDALA
                     VTDGKSSVMAMNPLVMSHLLQRRGFDILLHVSCRDRNRLALQSDLLGAAALGVSNLVV
                     ATGDYSSMGDHPQTKPVFDLDSVQLLKGIQKMESGQDLGGNPLNGRPKFFVGATVSPS
                     PGREAALELQLIRMEKKIAAGARFFLTQPVYDVAAFSRFMKRAASFGIPILAGVLPLK
                     SASMARFINKNLPGVFVPEPLIEEIGGAPDRVQASLGQCARLVQELREFCQGVHFIPA
                     GWERKVPMILEVARL"
     gene            2926..3306
                     /locus_tag="VEL68_02215"
     CDS             2926..3306
                     /locus_tag="VEL68_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ34815.1"
                     /translation="MGKILVVDDEPDMVEMIKAALEGAAHQVITAFNGQEGLNRARKE
                     KPDAIVLDIMMPVKDGFVACKELKEDPALKNIPVLILTAVSEHFANTRYAKSMGLDLD
                     AEDYIDKPVDPKLLLVRVNKLLGK"
     gene            3394..3936
                     /locus_tag="VEL68_02220"
     CDS             3394..3936
                     /locus_tag="VEL68_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ34816.1"
                     /translation="MSLPSETKSLSALEDFWGSEIHVQDCYQCQKCSAGCPVAFAMDY
                     KPNQIMQLVSLGMKERVLSSKTIWVCASCYTCSTRCPNDIDIAKVMDWLRQTAIREGV
                     PPAEKEVALFHSTFLESIRTHGRVHELSMMARYKQKTGKWMDDFKLGWRMFTKGKLKL
                     FPSRVREKKEIKDAFRGLKG"
     gene            4029..4859
                     /locus_tag="VEL68_02225"
     CDS             4029..4859
                     /locus_tag="VEL68_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HXZ34817.1"
                     /translation="MKVSYYPGCALHGTALEYDESTRAVSALLGVELGELPDWNCCGA
                     SSAHVTDDSLAYGLVARNLAIAEKEGRDLVVPCAACYSRFKAGEKEMEAGAHKLNFRI
                     LSLLEFLVEDGLMDRIKGMIKRPLTGLKMVNYYGCLLVRPPKVTGAKKYENPEEMDRL
                     LKLLGGEAIPWSYKTDCCGGSLVLTRTDIVRRLTQKLFDKALETGAEAIAVACPLCQS
                     NLDSRQEEISREAGKNYQIPILYITELIGLSLGHPDAGKWFRRHFVDPAKLLASKGLV
                     "
     gene            4873..9402
                     /locus_tag="VEL68_02230"
     CDS             4873..9402
                     /locus_tag="VEL68_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ34818.1"
                     /translation="MEKEKEAQRKDVEKIGSVMVVGGGVGGMQASLDLANSGFKVYLV
                     EESSAIGGRMAQLDKTFPTNDCSMCTISPKLVETGRHLNIQLMMDSEVVKVEGQAGNF
                     SATVRHKPRYIDISKCTGCSECAQVCPILMPGRFDEGMAQQRAAYKLYPQAVPNAYAI
                     EKLGVAPCRDACPAGQRAQGYIALIREGRYQDAMRVIKEDNPFPGICGRICNHRCEEA
                     CNRNLADQPLSIASLKRFVADQVYPLPYIPPDPLPYKFEEKVAIIGAGPCGLTAAQDL
                     RKLGYPVTIFEALPLAGGMLRVGVPDYRLPPKVVDREVREIIDLGIDLRLNTPVTDLD
                     EVMKEGFKSVLIAVGAHEGRKLPIPGANLPEVLINTQFLRDVSLRNIGIESGNGKPHP
                     KSVVQNRHVLVLGGGNVAMDCARTALRLGAAKVEMACLESREKMPASIEEIQEAEEEG
                     ITVYPSRSFKRILDKEGHVAGVEAVKVTFMEFDREGRLNLQTEEGSEHLLPCEVVIFA
                     IGQRAGLAFIPESTGVGVTRMSTIAVNPNTYAATRPGVFAAGDATTGTAFVIEAVAAG
                     HKAAASMHRYLRGEEMEPTPAPELPVVKMVKEEVQERVAKGEIKVTPRFKVAASPPGE
                     RVSSFGEVSLGYNEEEAKAEASRCLACGVCSECLCCYYKCGLNAINHDMVETQEEVKV
                     GSIILAPGYEVYNARLSQEYGLGRYPNVLSALQFERVLSASGPTLGHVQRPSDKKVPK
                     KIAFLQCVGSRDQNHPYCSAVCCMYATKEAIIAKEHEKEVEPTIFFIDIRAYGKGFDT
                     YYERAKKEYGVRYVRCAISRVVEDPRTKNLLITYLDEAGEIREEEFDLVVLSVGMVPA
                     ASTKELAKNVGIELDAYGFAKTDPLNPLATTRPGVYVCGVFQGPKDIPETVAQASGAA
                     AAASGILSAVRDTLVTRKEYPAQKEVKGEEPRIGVFVCRCGNNIGGVIDVPAVKDYAA
                     SLGAVVLADENLYTCSQDTQEKLKKAIEEHKLNRVIVASCSPRTHEPLFQETIREAGL
                     NRYLFEMANIRDQCSWVHMHDKEDATSKARDLVRMAIANARLIQPLEELTKGVIKRGL
                     VIGGGLSGMTAALGLAEQGFEAVLVEKEDELGGNLRRLKTTLDGKDVSHILEDLKSRV
                     TQHPRIQVFTNAVITDFGGYVGNYKTSLMVGPRMFTREVEHGVTLVATGGEELKPKEY
                     LYGEDKRVLTQLEMEAFLHGDGRKIEELKEVVMIQCVGSRNEERPYCSRVCCAEAVKN
                     ALALKALNPKARVTILYRDMRMYSTLEEDYARARKAGIRFLRYEEDRKPEVTQKDGRL
                     RVTFYNPVLRETLSLEPDLLVLSAATIAADTAELASMLKVPRTADGFFLEAHMKLRPV
                     DFASEGLYLCGIAHSPKTIEESLSQASAAVARACTVLAKDQIQISGVVSVVDADKCAA
                     CLTCVRVCPYNVPVVNKDGVAEIEAAMCHGCGICASECPGKAIRLQHFTDEQVMAKCD
                     VIVEVLSDVFKGERV"
     gene            9402..9881
                     /locus_tag="VEL68_02235"
     CDS             9402..9881
                     /locus_tag="VEL68_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HXZ34819.1"
                     /translation="MEDFEPIVIAFCCHYCAYTAADLAGTMRLQYPSNVRIIRLPCTG
                     KVDVRHLLEAFEKGADGVYVAGCMEGDCHFLKGNFRAKKRVAWAKKILEEVGIGGDRL
                     EMYNMSAAMGPRFAEVAREMTEKIRKLGPNPAKKAERKAPSAEGKEIEKERHAQGVT"
     gene            9964..10632
                     /locus_tag="VEL68_02240"
     CDS             9964..10632
                     /locus_tag="VEL68_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ34820.1"
                     /translation="MIIAEGKPIKEILAMIEGYDKIIVAGCKGCVTVCSAGGEKEVGI
                     LASALRLASQTRGKALETKEITLERQCDTEYLEPLREIADHYPVVISLACGAGIQFLA
                     EKFRKLPILPGVNTQFIGVTEEQGVWTERCQACGNCVLDRTGGICPVARCSKSLFNGP
                     CGGSKNGKCEIDSQVDCGWQLIYDRMKELGQLDKLEEPMPIKDWSTSRDGGPRKRVRE
                     DLKI"
     gene            10629..11555
                     /locus_tag="VEL68_02245"
     CDS             10629..11555
                     /locus_tag="VEL68_02245"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXZ34821.1"
                     /translation="MKSGSRLEKLLAGGHFVVTGELGPPKGADVAVVEKKANLLKGNV
                     DSINVTDNQTAIVRMSSIAVSALLIRMGLEPNMQMVCRDRNRIAMQSDIFGAYALGIR
                     NILCLSGDHQKFGNHPTAKNVFDLDSIQLIQTVKKMRDEHKVLGGDEIEGAPQMFIGA
                     AANPFADPFEYRVIRLGKKATAGVDFIQTQCIYDMDKFERFMKMVRDRGLHEKVFILA
                     GVTPLKSVGMAKYMKEKVAGMEVPDEIIERMRKAGKEKAREEGLSICVEQIQRLRQVP
                     GVHGIHLMAIEWEDAVHGIVERAGLLPRPEIL"
     gene            11712..13706
                     /locus_tag="VEL68_02250"
     CDS             11712..13706
                     /locus_tag="VEL68_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXZ34822.1"
                     /translation="MLVRFQPHNREIEVPAGENLLKAAVEAGVYIQASCGGEAFCGKC
                     RVKIEKGETEGPRTPKVSEEEYRQGFRLACQTRVRSDVEVLIPVESHLDRRVIARTRE
                     RVSAGRRVSHQELESLVLGWCYSPALRKQFVEVEPPTAKDNRSDLSRLLVALKKNCGL
                     EGISVDSRLLWRLPSILREADWKVTATLVQTRMESQLGEYQLRGSRRTKLINLEKGDL
                     AQEHYSIVLDIGTTTVWGQLLDLNRRKTLAEVSDYNAQIQYGDDVISRIVYSQKPGGL
                     KKLQESVVGTVNGLIREMVVKGGVDISRVSHMTAAGNTVMTHLLLGLDPKYIRESPYT
                     PVANYIPPIRAVEAGFEVGEHVHLYTFPSVASYVGGDIVSGVLGSGIYQRKPLTLYID
                     IGTNGEIVIGNSEWLVTAACSAGPAFEGGGIKHGMRATTGALEDFRINPVSYEPMLLT
                     IGMVKPKGICGSGLINMIAEFLEAGVISPNGKFNAELPTRRIRPGPDGREYVLAYAEE
                     TATGADLVITEVDIDNLMRAKAAMYAGYVTLLQSVGLSIASLEQVVIAGAFGSFIDLE
                     RAITIGLLPELPLDRFLFIGNGSLLGARLISFCNEMLDDGERISRMMTNIELSENPSF
                     MDNYIAALFLPHTNSAEFPGVSQKLKVRSSEFGVRKLEAL"
     gene            13728..14483
                     /locus_tag="VEL68_02255"
     CDS             13728..14483
                     /locus_tag="VEL68_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ34823.1"
                     /translation="MGKVIAMAGKGGTGKTTLSALVIGYLWKKGLKPILAVDADPSSN
                     LVDALGLTVNKSLGTAREDFFETKGNLPPGMTKETYLEMKLHEILVESPGLDLLVMGR
                     PEGPGCYCYANNILRRHLDVLIKNYPFVVMDNEAGMEHLSRRTTQGVDYLLFVSDYSL
                     RGIRTVGKIRELIDELKLSVKEKHLVVDRVPNGMDPVFSREVRDLGLNLLGAIPEDTF
                     ISEYEMRGRPLLELPDESPAVQVVSEMMEKMMK"
     gene            14534..14746
                     /locus_tag="VEL68_02260"
     CDS             14534..14746
                     /locus_tag="VEL68_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34824.1"
                     /translation="MAKNLEEKTLFSGEKVKVVGAEYEEGTPEQPHRWRGKIQSRQDM
                     LKYLQNGERYWFSKEWYGSEKRKTPA"
     gene            14857..15798
                     /locus_tag="VEL68_02265"
     CDS             14857..15798
                     /locus_tag="VEL68_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HXZ34825.1"
                     /translation="MDIPKVNYTGKIKEIAVGKGDKKITVGGESMYPFYLFEGQMPHP
                     PRIAMEIYDAPPEDWAEAALEPFRDVVNDPVAWAKKCVNAYGAEMVCLQLTSTDPNGM
                     NRPSEEAAAIAKKVAEAIDVPLIVWGCANDEKDAGTLRRVAELCADKNLVIGPVGEKN
                     YKQIGAGAIAYKHILAASSPIDVNLAKQLNVLLGNLGVPDGQILVDPTTGGLGYGIEY
                     TYSVMERDRMAALTQQDERLQFPLVCNLAKEVWKTKEAKLSQADAPNLGDPKKRGVLM
                     EAVTAVILLMAGANILVMRHPEAIRLVKGMIGELLKA"
BASE COUNT         3556 a         4368 c         5046 g         2920 t
ORIGIN      
        1 ctgatcatgc tccacgatga gtctttgggc tctctgacca cttccaagca gaggcaggca
       61 gtgggcacca tgctccggaa cgtgaacgcc ctgatcaaca tgatccaaaa gtacctccag
      121 ctgggccgca tcgaatcggg acggatggag gtgaacaaag tccagatgcc catcttcaac
      181 gaagccctgg cccccgttct cgaggatgaa aaacagcagc tggacgcccg ccggatgcag
      241 gtggtcctgg agaacgaaga ggccttccgc cgggtggagg aggaggccga cccggttctt
      301 ctgcgcattg tctttgacaa cctcatcggc aacgccatca aatacgggaa ggaaggggga
      361 acgatctggg ccggattccg cgaggaaccc acggaactct gtttctacgt gaaaaatgaa
      421 gggcggggga tccctgcgga taagctgaac gtggtctttg aaaaattcgc ccgcctggaa
      481 ggggagctgg aacgccggcg gaggggaacc ggcttgggat tgttcaatgc caagcagatc
      541 gtggaaaagc acggagggaa gatctgggcc gaatcggagg aggggaagtg ggccaatttc
      601 cttttcaccc tccccaaggg agtcaagggg agttgattcc catggatcgc cagtcacaga
      661 accatcaatg gagggagaga tggaggggcg caggaaggtt ttagtcatcg acgatgaacc
      721 ggacctggtg gaaatgattc ggatggcttt ggaaaggatg ttcgaggtga tcaccgcctt
      781 taacgggaag gaagggattg ccaaggcgcg gtccgagcgg cccgatgtca tcgtcctgga
      841 tgtgatgatg cccgaaaagg acgggttcac tgcctgccgg gagctgaagg aagacccgca
      901 aaccgcttcc atcccggtcc tcatcctcac gggggtggcg gaacatttcg ggcgcaccaa
      961 attccaccgc atgggcggaa tggagatcga ggcggaggac ttcatcccca agcccgtcga
     1021 tcccaacgag ctgctgcgtc gggtggcggc tcttctgaaa aaagggtgaa tcagaaagga
     1081 ttgcccctgg ggtgaccttc cgacccgtcc ggctccgggt tttggtttcg gggaagtcag
     1141 gaagcgcgtc atggaatttg aaaaagccgt cttgggagaa gaagaaatga ttgagttaga
     1201 acccttgaaa agcgaggaga ttgcccggaa tctggattcc caggcggagg tcttcttcat
     1261 cggctgcagc ggctggaagg aagtgtgtaa ccccggggga gaggcccagt tgcagctgct
     1321 gaagaaggac ctctccctgc ggggagtttc tttcaccggg accctggtcg ttgatgcgct
     1381 gtgtaacaag gggatggatg agctgagcct cctgacccag attcggcagg cctgccgatc
     1441 ccgtaccctt cttgtccttt cgtgcgaagt cggggttcgg gcgctggggg cggttgccgg
     1501 gcagcgggtg atgccggccc tgcggacctt ggcggcggaa ggattccaag gggtgctggg
     1561 ggacagggaa ctatgccgtt tgtgcggcga gtgcctgctg gaggcaagcg gcgggctctg
     1621 tcccctttat ttctgcccga aagagatgct caccggcccc tgccagggcg cccgccaggg
     1681 acaatgcgag gtggacccca agaccccctg cgggtgggag ttgatctatg accggctgaa
     1741 gactcagggg cgactggaga tgctcaagac ctctgccgaa ccgcgggacc acagcaaaat
     1801 ccttccgctt ctccgtctcc gatccactct ggcggcgggg agaaaatgaa attgcggatt
     1861 ggggattttg gattgcggat tgcaacccca cccgctccct tccccgcaag ggagcgccgg
     1921 ggatggaaat cgttccttaa tccgcaatcc gaaatcccca atccgcaatc gaatcggggg
     1981 taccgatgac ctttcaggaa agattgcagg agaataaatt cagcatcacc tgtgagatca
     2041 atcctcccaa aggggccgag gcggcggaaa ggctggaaga ggccgaaacc ctcaaggggc
     2101 gggtggacgc gcttgcggtc accgacggga aaagctcagt catggccatg aatcctctgg
     2161 tcatgagcca cctcctccag cggagaggct tcgacatcct tttacatgtg agttgtcggg
     2221 atcggaaccg actggccctg cagtccgatc tgctgggagc ggccgcactg ggggtcagca
     2281 atttggtggt ggccacgggc gactattcct ccatgggaga tcacccccag accaagccgg
     2341 tcttcgacct ggattcggtg cagttgttga agggcatcca gaaaatggaa agcggacagg
     2401 acctgggcgg gaatcccctg aacggccgac ctaagttttt cgtgggggcc accgtgagtc
     2461 ccagcccggg acgggaggct gcgctggagc tgcagctgat ccgcatggag aagaagattg
     2521 ccgcgggggc caggttcttc ctgacccagc cggtgtatga tgtcgcggcc ttctcccggt
     2581 tcatgaaaag ggcggcttct tttggaattc ccattttggc cggtgtgctg cccttgaaat
     2641 cagcctccat ggcccggttc atcaacaaaa atttgcccgg ggttttcgtt cccgaaccgc
     2701 tcatcgaaga aatcggcggc gctccggacc gggtccaggc atccctgggc cagtgcgccc
     2761 gtttggtgca ggaactgcgg gaattttgtc aaggggtcca tttcatcccc gccggatggg
     2821 aacggaaggt cccgatgatt ctggaagtgg cccggcttta ggggagggca tgggaatcca
     2881 tcgttcttgg gggctaattt aaatcattta ctgaaggaga gaaccatggg taaaattcta
     2941 gtagtcgatg atgaaccaga tatggtagaa atgataaagg ctgccttaga aggcgccgcc
     3001 caccaagtta taacggcttt taatgggcag gaaggattga accgggcccg gaaagagaag
     3061 cccgatgcca tcgttctgga tatcatgatg ccggtcaagg atggcttcgt ggcttgcaag
     3121 gagttgaagg aggacccggc tttaaaaaac attccggtcc tcatccttac tgcggtgagc
     3181 gaacatttcg ccaacactcg ctatgccaag agcatgggcc tggacctgga tgcggaagat
     3241 tatatcgata agcctgtcga tccaaagctg ttgctcgttc gtgtgaacaa attattgggg
     3301 aagtagagga aaaatcgcgg ttggcctgca agccgtagcc agagaggtgc ggcgaagggg
     3361 gaagattgcg attttctggc aaaggaggat ttcttgtccc tacccagcga aacgaaatct
     3421 ctttctgcat tggaagattt ctggggcagc gagattcacg tccaggattg ctaccaatgt
     3481 cagaagtgct cggccggctg cccggtggcc ttcgccatgg attacaagcc caaccagatc
     3541 atgcagctgg tctccttggg gatgaaagaa cgagtcctct cctccaaaac gatatgggtc
     3601 tgcgcctcct gttacacctg ttccacccgc tgccctaacg acatcgacat tgccaaggtc
     3661 atggactggc tgcgtcagac ggccatccgc gaaggcgttc cccccgcgga gaaggaagtg
     3721 gccctcttcc actccacctt cctggagtca atccgtaccc atggccgggt tcacgaactt
     3781 tccatgatgg cccgttacaa gcagaagacc ggaaagtgga tggatgactt caagctgggc
     3841 tggaggatgt ttaccaaggg gaaactcaag ctttttccct cgagggtgcg ggagaagaaa
     3901 gaaataaagg atgcctttcg gggattgaag ggatgagaat cattgtaagg gcgaccggcc
     3961 ggtcgcccgt acggagtccg gagaaaaaaa caaaattttg atcgggaagt ccgagtgagg
     4021 gaccgaagtt gaaagtttcc tattaccctg gctgtgcttt acacggcacg gcgctggaat
     4081 atgatgagtc gacccgggcg gtgagcgcgc ttctgggagt ggagctcggc gagctgcccg
     4141 actggaactg ctgcggggcc agctcggccc acgtgaccga tgactctctg gcctatggct
     4201 tggtggcccg aaacctggcc atcgcggaga aagaaggaag ggacctcgtc gtcccctgcg
     4261 cggcctgcta cagccgcttc aaggcgggag agaaggaaat ggaagcgggg gcccacaagc
     4321 tcaacttccg gattctgagc ctcctggagt ttctggtcga ggacggcctc atggaccgga
     4381 tcaaagggat gatcaaacgg cccctcaccg gtctcaagat ggtcaattat tacgggtgtc
     4441 tcctggtccg ccctcccaag gtgaccgggg ccaaaaaata tgaaaacccg gaggagatgg
     4501 accggctcct gaaactcctg gggggggaag ctatcccctg gtcttacaag accgactgct
     4561 gcgggggaag cctggtcctc accaggacgg acatcgtccg gcgcttgacg caaaagcttt
     4621 tcgacaaggc cctggagacg ggggcagaag ccattgccgt ggcctgtccc ctgtgtcagt
     4681 ccaacctgga ttcccgccag gaagagattt cccgggaggc gggaaagaat taccagattc
     4741 ccatcctgta catcaccgaa ttaatcggtt tgagcctggg ccatcccgat gctgggaaat
     4801 ggttccggcg gcatttcgtc gacccggcga agcttttggc ttccaaggga ctggtttgag
     4861 ggcaacgaga gaatggaaaa agagaaagag gctcagcgaa aggacgtgga gaaaatcggt
     4921 tcggtcatgg tcgtgggcgg cggcgttgga gggatgcaag cctccctgga cctggccaac
     4981 tcgggcttca aagtttatct ggttgaggag agctcggcca tcgggggccg catggcccag
     5041 ctggacaaga ccttccccac caacgactgt tccatgtgca ccatcagccc caagctggtg
     5101 gagaccggcc gccacctgaa catccagctt atgatggaca gcgaagtggt gaaagtggaa
     5161 ggccaggccg ggaacttctc ggccacggtg cgccacaagc cccgctacat cgacatcagc
     5221 aagtgcacgg ggtgcagcga gtgcgcccag gtctgcccca tcctcatgcc cgggagattc
     5281 gatgaaggca tggcccagca gagagcggcc tacaagctct acccccaggc cgtgcccaac
     5341 gcctacgcca tcgagaaact cggcgttgct ccctgccggg acgcctgccc cgcaggccag
     5401 cgggcccagg ggtacatcgc cctcatccgg gagggacgct accaagacgc catgcgggtg
     5461 atcaaggagg acaatccctt tccgggaatc tgcggacgga tctgcaacca ccggtgtgag
     5521 gaggcctgca accgcaacct ggcagatcaa cccttatcca tcgcctcttt gaaacgcttc
     5581 gtggccgacc aggtctatcc actcccgtac atccctccgg acccccttcc ttacaagttc
     5641 gaggagaaag tggccatcat tggcgccggc ccctgcggcc tgaccgccgc ccaggacctg
     5701 cgcaagctcg gctatccggt caccatcttc gaggccctgc cccttgccgg gggaatgctc
     5761 cgggttgggg tccccgacta ccgcctgcct ccgaaggttg tggaccggga ggtgcgggag
     5821 atcatcgacc tgggaatcga cctgcggctc aacaccccgg tgaccgacct cgacgaggtg
     5881 atgaaggaag gtttcaagtc ggtcctgatc gcggtggggg cccatgaggg tcgcaagctg
     5941 cccattcctg gtgccaacct tcccgaagtc ttgatcaata cccaattctt acgggacgtg
     6001 agtctccgca acatcggaat tgaatcgggc aacgggaagc cccacccgaa gtcagtcgtc
     6061 cagaatcgtc atgtcctggt tctcggaggg gggaacgtag ccatggactg cgcccgaacc
     6121 gccctccgcc tgggcgcggc caaggtagaa atggcctgtt tggagagccg ggaaaaaatg
     6181 ccggccagta tcgaagagat tcaggaggcc gaagaagaag ggatcaccgt ctaccccagc
     6241 cgctccttca agcgaatcct ggacaaggaa ggccacgtgg ccggcgtgga agctgtcaag
     6301 gtaaccttca tggagtttga ccgcgaaggc cgcctgaacc tgcagaccga ggaagggagc
     6361 gaacacctcc tgccttgcga agtagttatt ttcgccattg ggcagcgggc cggcctggcc
     6421 ttcatccccg agagcacggg ggtgggagta acccggatgt ccaccatcgc agtcaatccc
     6481 aatacctatg ccgccacccg tccaggggtc ttcgccgccg gcgacgccac gacgggcacg
     6541 gcctttgtca tcgaagccgt ggctgcgggg cacaaggcgg cggccagcat gcaccggtac
     6601 ctgcgggggg aagagatgga accgacccct gcgcccgagc tgccggtggt aaagatggtc
     6661 aaggaagaag tccaggagcg ggtggccaaa ggagaaataa aggtcactcc gcggttcaag
     6721 gtagccgcga gtcctcccgg tgagagggtg tcttcctttg gggaggtgag cctagggtac
     6781 aacgaggagg aggccaaggc ggaagcatcc cgttgtctgg cctgcggggt ctgctccgaa
     6841 tgcctctgct gctactacaa gtgcgggctc aacgccatca accatgacat ggtggagacg
     6901 caggaagaag tcaaggtcgg gtccatcatc ctggccccgg gctacgaggt ctacaacgcc
     6961 cgtctttcgc aggagtacgg cttggggcgg tatcccaacg ttttgagcgc cctccagttc
     7021 gagcgagtcc tctccgcttc cgggccgacc ctgggccacg tccagcgacc gtcggataaa
     7081 aaggtgccca agaagatcgc cttcctccag tgcgtgggga gccgggatca gaaccatcct
     7141 tattgctcgg cggtctgctg catgtacgcc accaaggagg cgatcatcgc caaggagcac
     7201 gaaaaggaag tcgagcccac gatcttcttc atcgacatcc gcgcctacgg aaaggggttc
     7261 gatacctatt acgaacgggc caaaaaggaa tacggggtcc gctacgtgcg ctgcgccatc
     7321 tcccgggtgg tggaggaccc gcgcacgaag aacctgctga tcacctatct cgacgaagcg
     7381 ggggaaatca gggaagagga gttcgacctg gtggtcctct cggtaggcat ggtgcccgca
     7441 gcctccacca aagagctggc aaaaaacgtg gggatcgaac tcgatgccta cggttttgct
     7501 aaaaccgacc ccttgaatcc cctggccacc acccgaccgg gcgtgtacgt gtgcggggtg
     7561 ttccagggac ccaaggatat tcccgagacc gtggcccagg ccagcggggc tgcggcagca
     7621 gcctccggga ttctgtccgc ggttcgggac accctggtaa cgcgcaagga atatccggcc
     7681 cagaaggaag tcaaggggga agaaccgcga atcggcgttt tcgtctgccg ctgcgggaat
     7741 aatatcggcg gggtcatcga tgtgccggcg gtgaaggatt acgccgcctc cctgggcgcc
     7801 gtggtcttgg ccgacgagaa tctctacacc tgctcccagg atacccagga gaagctcaaa
     7861 aaggccatcg aagagcacaa gctcaaccgg gtgatcgtgg cctcctgctc ccctcggacg
     7921 cacgagcccc tcttccagga aaccatccgg gaggccgggc tcaaccgcta cctcttcgag
     7981 atggccaaca tccgggacca gtgctcctgg gttcacatgc acgataaaga ggatgccacc
     8041 tccaaggccc gcgacctcgt gcgcatggcc atcgccaacg cccggctgat acagcccctg
     8101 gaagagttga ccaagggagt catcaaacgg gggctggtca tcgggggagg cctctccgga
     8161 atgaccgcag ccctgggcct ggccgagcag gggtttgagg cggtcctggt ggagaaagag
     8221 gatgagttgg gaggaaacct ccggcgcctt aagacgaccc tggatggaaa agatgtgtcc
     8281 cacatccttg aggaccttaa gagccgcgtc acccagcatc cccgcataca ggtcttcacc
     8341 aacgcggtga ttaccgactt tgggggttac gtgggaaact ataagaccag cctcatggtg
     8401 gggccccgga tgttcacccg ggaagtagag cacggggtga ccctggtggc caccggaggc
     8461 gaggaactta agcccaagga atatctctac ggggaggaca agagagtcct cacccagctg
     8521 gagatggaag cgttcctcca tggggacgga aggaagattg aagagctgaa agaagtggtc
     8581 atgatccagt gcgtgggatc gcggaacgag gagcggccct attgcagccg ggtgtgctgc
     8641 gcagaggcgg tgaagaacgc cctggccctc aaggcgctca atcccaaggc cagagtgacc
     8701 attctgtacc gtgacatgcg catgtatagc accctggaag aagactacgc ccgcgcgcga
     8761 aaggccggaa ttcgcttcct ccggtatgaa gaggaccgca agccggaggt gacccaaaaa
     8821 gacgggcggc tccgggtgac tttctataac ccggttcttc gggaaaccct ctctctcgag
     8881 cccgaccttt tggtcctctc cgcggccacc atcgcggcag acacggcgga gctggccagc
     8941 atgctcaaag tgccccgcac cgccgacggc ttcttcctgg aggcccacat gaagctgcgg
     9001 ccggtggact ttgcctccga ggggctctat ctctgcggca tcgcccattc gcccaagacc
     9061 atcgaggaga gcctgtccca ggcgtcggcg gcagtggccc gggcctgcac ggtcctggcc
     9121 aaggatcaga tccagatcag cggggtggtt tcggtggtgg atgcggacaa gtgcgctgcc
     9181 tgcctgacct gcgtacgggt ctgcccctac aacgtgccgg tggtcaacaa ggacggggtg
     9241 gcggagattg aggcggccat gtgccacgga tgcggcatct gcgcttccga gtgcccgggg
     9301 aaggccatca gactgcagca cttcaccgac gagcaggtca tggccaagtg cgacgttatc
     9361 gtcgaggtcc tgtccgacgt cttcaaggga gaaagggtct gatggaagat ttcgaaccca
     9421 tcgtcatcgc cttctgctgc cactactgcg cctacaccgc ggcggacctg gccgggacca
     9481 tgcgcctcca gtatccctcc aacgtgcgga tcatccgcct tccctgcacc gggaaggtgg
     9541 atgtgcgcca tctcctggag gcttttgaaa agggggcgga cggagtatac gtggccggct
     9601 gtatggaggg agactgccat tttctcaagg gaaacttccg ggccaagaag cgggtggcct
     9661 gggccaagaa gatcctggag gaggtgggca tcggggggga ccggctggag atgtacaaca
     9721 tgtccgcggc catgggcccc cgctttgccg aggtggcccg ggagatgacc gagaaaatcc
     9781 gcaaactggg gccgaatccg gcgaagaaag cagagcgcaa agcgcccagc gcagaaggta
     9841 aggaaataga aaaagagcgt cacgcccagg gcgtgacttg agcgaagcgc tcctcgaacc
     9901 atgcaagttg gcgaatgaac gcaaggtgtg aaagctgaaa gagaattact caggagtgaa
     9961 ctcatgatca tcgctgaagg aaaacccatt aaagaaatat tggctatgat tgaaggctac
    10021 gacaagatca tcgtggccgg atgcaaggga tgcgtgaccg tctgttccgc gggaggggag
    10081 aaggaggttg gcattctcgc ttccgccctc cgcctggcca gccagacccg gggcaaggcc
    10141 ctggagacga aggagatcac cctggagcgc cagtgcgata cggaatacct ggagcccctg
    10201 cgggagatcg ccgaccacta ccccgtagtt atttccctag cctgcggcgc gggaatccaa
    10261 ttcctggccg agaagttccg caagcttccc atcctccccg gagtcaacac tcagttcatc
    10321 ggggtgaccg aggagcaggg ggtctggacc gagcgttgcc aggcctgcgg gaactgcgtc
    10381 ctggaccgga ccggcggaat ctgcccggtg gcccggtgct ccaagagcct cttcaacggg
    10441 ccctgcggcg gctccaagaa cggcaagtgc gagatcgatt cccaggtgga ctgcggctgg
    10501 cagttgatct atgatcggat gaaggagctg ggccaactgg ataaactgga agaacccatg
    10561 cccatcaagg actggtccac cagccgggac ggggggccgc ggaaacgagt gagggaggac
    10621 ctgaaaatat gaagtccggc agtcgattgg aaaagctcct ggccgggggg catttcgtgg
    10681 tcaccgggga gctggggccg cccaagggag ccgatgtggc ggtcgtggaa aagaaggcca
    10741 atctattaaa agggaacgtg gattccatca atgtgaccga caaccagacg gccatcgtac
    10801 ggatgtcttc catcgcggtg agcgcccttc tgatccgcat gggactggaa cccaacatgc
    10861 agatggtctg ccgggaccgg aaccgcatcg ctatgcagag tgacattttc ggggcctatg
    10921 ccctgggcat ccgcaacatc ctctgcctat ccggagacca ccagaagttc ggaaaccacc
    10981 ccacggccaa gaacgtcttc gacctggatt cgatccagct gatccagacg gtcaagaaaa
    11041 tgcgggacga acacaaggtc ctgggagggg acgagattga gggggccccc cagatgttca
    11101 tcggcgccgc ggccaacccc ttcgccgatc cctttgagta ccgggtcatc cggctgggca
    11161 agaaggcgac ggccggagtg gatttcatcc agactcagtg catctacgac atggacaagt
    11221 tcgaacggtt catgaagatg gtgcgcgacc gagggctgca tgaaaaagtg ttcattctgg
    11281 ccggagtgac cccgctgaaa tccgtgggca tggccaagta catgaaggag aaagtggccg
    11341 ggatggaagt cccggacgaa atcatcgagc gaatgcgcaa agccggaaaa gagaaggccc
    11401 gggaagaagg gctcagcatc tgcgtggagc aaatccagcg gctgcgccag gtcccggggg
    11461 tccacggcat ccatcttatg gccatcgaat gggaggatgc ggtgcacggg atcgtggagc
    11521 gagccgggct gctgccgcgc ccggaaatac tgtaatttaa taccaacgca ataaaaatag
    11581 ggttacaaag gatgtagggt gcgttccccg aacgcaccgt gcaaatgatt atatgcgttc
    11641 ggtcatcccc gtcttccatg gacggcggta aaattataat agagaaggag ccagtccttc
    11701 tgccgaaccg agtgctcgtt cgtttccaac ctcataaccg agaaattgaa gttcccgcgg
    11761 gggagaacct tctcaaggcg gcggtggaag caggggtcta tatccaggcc tcctgcggcg
    11821 gagaggcctt ctgcggaaaa tgccgagtga agattgaaaa gggggaaacc gagggccccc
    11881 ggacccccaa ggtctccgag gaagagtacc gccaagggtt tcgcctggcc tgccagaccc
    11941 gggtcaggag tgatgtggaa gttctcattc cggtggaatc ccatttggac cgccgggtga
    12001 ttgcccgaac ccgggaacgg gtgagtgccg gtcgacgggt ctcccaccag gagctggagt
    12061 ctttggtcct ggggtggtgt tatagcccgg ccttgcgcaa gcagtttgtg gaggtggagc
    12121 cgcccaccgc caaggacaac cggtcggacc tttcccggct cctcgtggcc ctgaaaaaga
    12181 actgcggcct ggaggggatt tcggttgatt cccgcctcct ttggaggctg ccttctattt
    12241 tgcgggaggc ggactggaaa gtgaccgcca ccctggtcca aacccgcatg gaatcccagc
    12301 tgggtgagta tcagctccgc ggctcgcgga ggacaaaact catcaacctg gagaaagggg
    12361 accttgccca ggagcattat tccatcgtcc tggacattgg aacgactacc gtttggggcc
    12421 agctgttgga tttgaaccgg agaaagaccc tggccgaggt ttcggattac aacgcccaga
    12481 ttcagtacgg agacgatgtg atctcccgga tcgtctattc ccagaagccc gggggcctga
    12541 agaagctcca ggaaagcgta gtgggtacgg ttaacggact cattcgggaa atggttgtca
    12601 aggggggagt ggacatctcc cgggtctccc acatgaccgc agcgggcaat acggttatga
    12661 cccatctcct tcttggcctg gacccgaagt acataaggga gtccccttac accccggtgg
    12721 ccaattatat tccgcccatc cgggccgtgg aagctggatt tgaagtgggg gagcatgtcc
    12781 acctgtatac ttttccttcg gtggcttctt acgttggggg ggacattgtt tcgggggtct
    12841 tgggctcggg catttaccag cgcaagcccc tgaccctcta catcgacatc gggaccaacg
    12901 gggagatcgt catcggaaat tcggagtggc tggtgaccgc ggcctgctca gccggtccgg
    12961 ccttcgaagg gggggggatc aaacacggga tgcgggcaac caccggggcg ctggaggatt
    13021 tccgaatcaa ccccgtctct tatgagccca tgctcctgac catcgggatg gtcaagccca
    13081 agggcatctg cgggtccggg ctgatcaaca tgatcgccga gttcctggaa gccggggtga
    13141 tctcgcccaa cgggaaattc aacgccgaat tgcccacccg gagaatccgc cccgggccgg
    13201 atggaaggga gtacgtcctg gcttacgcgg aagagactgc caccggtgcg gacctggtca
    13261 tcaccgaagt ggacattgac aacctgatga gggccaaagc ggccatgtac gccgggtatg
    13321 tgaccctttt gcagtccgtg gggctctcca tcgccagtct ggagcaggtg gtgattgccg
    13381 gcgctttcgg ctccttcatc gacctggaac gggccatcac catcggtctt cttcccgagc
    13441 ttcctctgga ccggtttctt ttcatcggca atggctccct gctgggggcc cggctgattt
    13501 ccttctgcaa cgagatgctg gatgacgggg agaggatctc ccggatgatg accaacattg
    13561 aattgagcga gaatccctcg ttcatggaca attacatagc cgctttgttc ctgccccaca
    13621 cgaattcagc cgagttcccg ggggtgagcc agaagttaaa agttcggagt tcggagttcg
    13681 gagttagaaa acttgaggca ctataggcag ttaatgcacg gaatattatg ggtaaagtga
    13741 ttgccatggc gggaaaaggg gggacgggga agacgaccct ctcggccctg gtcattggat
    13801 acttatggaa aaagggtttg aagcccatcc tggcagtgga tgccgacccc agctccaacc
    13861 tggtggatgc gcttggactg acggtgaaca agagcttggg gacggcgcgg gaggattttt
    13921 tcgagaccaa gggaaatctg cccccgggga tgaccaaaga gacctacctg gagatgaaat
    13981 tgcacgagat tctggtggag tctccggggc tggacctcct ggtcatgggc cgcccggaag
    14041 ggccgggatg ttactgctac gccaacaaca tcctgcgccg tcacttggat gtgctcatca
    14101 aaaactatcc cttcgtggtc atggacaacg aagcggggat ggaacacctc agccgcagga
    14161 ccactcaggg agtggattac cttcttttcg tgtccgacta ctccctgagg ggaattcgca
    14221 cggtaggcaa gatcagggaa ctgatcgacg aattgaaact ctccgtgaaa gaaaaacatc
    14281 tagtggtgga ccgggttccg aatggaatgg accccgtctt ttcccgggag gtccgggacc
    14341 tggggcttaa cctgctgggg gcaatccccg aggatacgtt tatatctgag tatgaaatga
    14401 gagggagacc cctgttggaa ctgccagatg aatcaccggc ggtgcaggtg gtgtccgaga
    14461 tgatggaaaa aatgatgaaa tgaacattca gcaccaaaaa cacaaaccga ttcagagaag
    14521 ggggggagag aggatggcca agaacttgga agagaagacg ctcttcagcg gggaaaaggt
    14581 gaaggtggtc ggggcggaat acgaggaggg gacgcccgag caaccgcacc gctggagggg
    14641 caagatccag agccggcagg atatgctcaa gtacctccaa aacggcgaac ggtattggtt
    14701 cagcaaagag tggtacggga gcgagaagcg gaagaccccg gcatgaaaac atagtgaaag
    14761 tgtaagtgta agttcaaagg aaagcatcaa tcagcagagc taaaaggcct ggaactttcc
    14821 catgcactta aactttcttt tccaatggag gtaaccgtgg acatacctaa agtcaactac
    14881 acgggcaaga ttaaagagat tgccgtcggg aaaggggata agaagatcac cgtgggcggg
    14941 gaaagcatgt accccttcta tctcttcgaa gggcagatgc cccatccccc gcggatcgcc
    15001 atggagattt acgacgcccc gccggaggat tgggccgagg ccgctctgga gcctttcagg
    15061 gacgtggtga atgaccccgt ggcctgggct aagaagtgcg tgaatgccta tggggccgag
    15121 atggtctgtc tgcagctaac tagcacggac cccaacggca tgaatcggcc ttcggaagag
    15181 gccgcggcca tcgccaagaa agtggccgag gccatcgacg tccccctcat cgtgtggggc
    15241 tgcgccaatg acgagaagga cgccggcacc cttcggcgag tggccgagct gtgcgcggat
    15301 aagaatctgg tgatcggccc ggtaggagag aagaattaca agcagatcgg ggccggggcc
    15361 attgcttaca aacacatcct ggcagcgtct tcccccatcg atgtcaacct ggccaaacag
    15421 ctcaacgtgc ttttgggaaa tctgggggtt cccgacggac agatcctggt ggaccctacg
    15481 accggcggac tggggtacgg gatcgagtat acctattcgg tcatggaacg ggaccgcatg
    15541 gccgccctga cccagcagga tgagcgcctg cagttcccgc tcgtctgcaa cctggccaag
    15601 gaggtctgga agactaagga ggccaagctg tcccaagcgg acgcccccaa cctcggagac
    15661 ccgaagaagc gaggggtcct gatggaagcg gtcacggcgg tgatcctgct catggccggg
    15721 gccaatatcc tggtcatgcg ccatccggaa gccatccgcc tggtgaaggg gatgatcggg
    15781 gaacttttaa aagcatagag taggggcgat tcatgaatcg cccctacaag aaatggagga
    15841 gggttatttc gatgtcagaa gtcacaaaaa gcgtagatca agccacgctg
//
LOCUS       DASPRR010000064         5268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_666951, whole genome shotgun sequence.
ACCESSION   DASPRR010000064 DASPRR010000000
VERSION     DASPRR010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666951"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VEL68_02270"
     CDS             <1..556
                     /locus_tag="VEL68_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34826.1"
                     /translation="KGKRAGQSFRWLLILVLILFLTASCATKRYWGPSMGRSADSQST
                     PADALQKALKTASFEGHKGKSLWIEVYTLTDRTGEESAEERFLRSWMAEKAASQGSRM
                     ARSKAEADILLDVKARVFGVSQTRRDFIPLVYMESTQGVVDLHLTFYERESGKILQTE
                     DLKGEARYLEYYILYMIGPFKSIN"
     gene            566..1111
                     /locus_tag="VEL68_02275"
     CDS             566..1111
                     /locus_tag="VEL68_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34827.1"
                     /translation="MTAHPFLWQTRIPSLLLLFLFLLSSCATVDYLPPQQGTSAELQS
                     ILAQSLEGNLKDIGFDPVGKTVDIQVRALGGHQTPLGLEKYVKSLLQEWAVKKGGKVG
                     PGEWKMEVFLPVLGTTATRRDLSFQYVPLYYSERFRAAGQLMVVVKDGEGKTAGIWLA
                     GKGGDLADMYLMRMLGPLTSP"
     gene            complement(1246..2118)
                     /locus_tag="VEL68_02280"
     CDS             complement(1246..2118)
                     /locus_tag="VEL68_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXZ34828.1"
                     /translation="MDKLIITVAIIGGITTREKTPHPPMTPREIADSAIEACQAGASV
                     CHIHVRDPITHQPSMKFELYKEVFDRIRDKCDMIINLSTGSGGRLYHNPKAAGNPWDT
                     SGLKSPEERVEHVVKLKPELCSLDIGSLNFGPYAFINLVSVVEKMSKMIRDVGTKPEL
                     EVFDLGHIRIGHHLIGQGLIEDPPLFQLCLGIPWGIEATTENMVYMRNNLPSGCLWYA
                     FAIGPQHFPMAAASIINGGHARVGFEDNLYIKKGVLAKTNAEMVKKTVEIAKLFDREV
                     ATPKEARQILRLKS"
     gene            2574..3542
                     /locus_tag="VEL68_02285"
     CDS             2574..3542
                     /locus_tag="VEL68_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXZ34829.1"
                     /translation="MAAVGKEVGLKIYRNMVRLRLLDDKINEMVASGIGITQHSTRGQ
                     EATTVAACAALEPQDYVMPYHRGWGWAIGKGMDPKILLAELIGKQPGCCTGKGGVHLA
                     DWNLRIMGRPGVQAAHVPIAAGVGLSIKFRKTRDVVLCFFGDGASNEGNVHEGMNLAS
                     VWKAPVIFICENNLYALFTPNVETTSVRDIADRAKGYAMPGVIVDGNDAMAVYETVRE
                     AIQRARSGQGPTFIESKTYRMHGHTAMDRFHLGGYRPKEEVEEWAKKDPVIGLRIRLL
                     EMKAASEADLKGIEAAAKEEIEDAEKFARESPYPTKEELFRDTYVE"
     gene            3559..4533
                     /locus_tag="VEL68_02290"
     CDS             3559..4533
                     /locus_tag="VEL68_02290"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXZ34830.1"
                     /translation="MGSREMTYAEAIREGLREEMRKNQEIILLGEDVKINVWTVTRDL
                     YKEFGDRVINTPLSESGFCGAAVGAALTGMRPVVEIMYPDFTMYAADSIANQAAKYRY
                     MCGGGPFKVPVVFRIAGGGSSNGAGCHHGQSLEATFLHFPGLKVVFPSTPHDAKGLIK
                     TAIRDDNPVLFYEHKLLYGVKGPVPEEEYTLPMGKAEVKREGSDVTIVSYAWTLHKVL
                     RAAEQLAKEGIEAEVIDLRSLRPLDKECILHSLKKTAKLVTAEEGTKMGGVGAEIAAV
                     VAEEGLHLLDAPVKRLAMEEVIIPSSRYGDQLFIPQESDIVAAVKDLF"
     gene            4676..>5268
                     /locus_tag="VEL68_02295"
     CDS             4676..>5268
                     /locus_tag="VEL68_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HXZ34831.1"
                     /translation="MAVEVKLSSLGVSITEGTIVRWLKKEGDRVEKGEILAEVETEKV
                     NFEIVAPEAGVLLKILYGEKTIAKAEATVALVGREGEDVSAYLEKVRQERKEAGEKVA
                     PSEKREKVAGGKTRAFPAAKFLAKEHGVDLAEVTGTGPGGQVTKEDVEKFIARKQPRR
                     EEGIPELEEILPLVGIRKAIADGMSLSVRTAAHVTTVA"
BASE COUNT         1207 a         1355 c         1615 g         1091 t
ORIGIN      
        1 aaaagggaaa agagcgggtc aatcctttcg atggctgttg atcctggtgc tgatcctatt
       61 tctgacggcc tcctgtgcga cgaaaaggta ctgggggccg agcatggggc gctcagccga
      121 ctcccaatcc actccggcgg atgcgctgca aaaggccctg aaaacggcat ccttcgaggg
      181 tcataaggga aaatccctgt ggattgaggt ttacacccta accgaccgga ccggagagga
      241 aagcgcggaa gagcgtttcc tccgttcctg gatggctgaa aaggccgctt cccaggggag
      301 ccgcatggcc cggtccaaag ccgaagcgga tattctcctg gatgtgaaag ccagggtctt
      361 cggggtttct cagacgcgcc gcgacttcat ccccctggtt tatatggaat ccacccaggg
      421 ggtcgtggat ttgcatctga ccttctatga gcgggaatcg gggaagatcc ttcagacgga
      481 ggatttgaaa ggcgaagccc ggtacctgga gtattacatt ctctacatga tcggtccatt
      541 caagtcaatt aactagggtg agaatatgac cgcccatccg ttcctgtggc agacccggat
      601 tccttctctg cttctccttt tcctgtttct tctttcctcc tgtgccaccg tggactatct
      661 gcccccccaa caaggcacat ccgccgagct gcagtcgatc ctggctcaat ccctggaagg
      721 gaacctgaaa gacattgggt ttgatcccgt gggtaagacg gtggatattc aagttcgggc
      781 cctgggaggg caccagactc ctttgggcct ggagaaatat gtgaagagcc tcttacagga
      841 gtgggctgtc aaaaaaggag gaaaggtggg cccgggggaa tggaagatgg aggtttttct
      901 ccctgtactg gggaccacgg ccacgcgaag ggacctgagc ttccagtacg tccccctata
      961 ctatagtgag aggttccggg cggccggaca gctcatggtg gtggtgaaag acggggaggg
     1021 aaaaactgcg ggaatctggc tggcggggaa ggggggcgac cttgccgata tgtatctgat
     1081 gagaatgtta gggcctttga cgtcccctta ggagatcagt gaccttccaa acattcttgg
     1141 tttttccgga agactttagc gtattgaaaa ggccttccca gaaatccctt cctttctccc
     1201 tgtttttcga agagaagaaa gctttggggg gtccggcatt ggatatcagc ttttcagcct
     1261 cagaatctgc cgcgcctctt tgggagtcgc cacctcccga tcgaagagtt tggcgatctc
     1321 gaccgttttt ttcaccatct cggcgttggt cttggccaat acccccttct tgatgtaaag
     1381 attgtcttcg aaccccaccc gggcgtgccc cccgttgatg atcgaggctg cagccatagg
     1441 gaagtgctgc gggccgatgg caaaggcgta ccacaaacac ccgctcggga ggttgttgcg
     1501 catgtaaacc atgttttccg tggtcgcttc aatcccccag gggataccca ggcagagttg
     1561 gaagagggga gggtcttcga tcagcccttg gccgatcagg tggtgaccga tgcggatatg
     1621 gccgaggtcg aagacttcca actccggctt ggttcctacg tcccggatca tcttggacat
     1681 cttttccacc acggacacca ggttgatgaa ggcgtagggg ccaaagttca aagagccgat
     1741 atccagggag cagagttcgg gcttcagttt caccacatgc tccacccgct cctccgggct
     1801 tttcaggccg gaagtatccc agggatttcc cgccgccttg gggttgtgat agaggcgtcc
     1861 gcctgagccg gtggataagt taataatcat gtcgcactta tcccggatgc ggtcgaagac
     1921 ttccttatag agttcaaatt tcatcgaggg ctgatgggtg atcggatcgc ggacatgaat
     1981 atggcagacc gaggctccgg cctgacaggc ctcaatagcg ctgtccgcga tctcccgggg
     2041 agtcatgggg ggatgagggg ttttttcccg ggtggtgatt cctccgatga tggcgaccgt
     2101 gatgatcagc ttgtccatgc cttttcctcc gcagtctcaa aggtaaaccc cccggccgag
     2161 acgaagggct gattttctat aaatgaatta gaatggaatg tcaaatgaat ttccaggaga
     2221 gtaagtcgtc accaaatcgc cttcggggcc aacccgtatt tccgccccgc ctcttatcag
     2281 agccttacgc gtactctgtt ctatgaaaaa ttttttacgc tatgaaaagt acggaagttg
     2341 gatttccatc ggcagaaacc taaggctatc gcggctttct catgttcagg tcccaatgcg
     2401 caaagacatt ccttccccca aatgatcttt ccgttgccgg actcaatttt cggattgcaa
     2461 aaatcccccg gatgtaatat tttggcctga cggaacgaag tcagattggg ataaagaaga
     2521 ggacggaacg gtttgccgtc ctttttcttc caaccctaag taggaggttt cctatggcag
     2581 cggtagggaa agaagtcggg ttgaagattt accggaacat ggttcgattg cggctcctgg
     2641 acgacaagat caacgagatg gtcgccagcg ggatcggaat cacccagcat tccacccgcg
     2701 gccaggaggc gaccacagtg gccgcctgcg ccgctctgga gccgcaggat tacgttatgc
     2761 cctaccaccg agggtgggga tgggcgatcg gcaaaggtat ggaccccaag attttactgg
     2821 ccgagctgat cggcaagcag cccggctgct gcaccggcaa agggggagtc cacctcgccg
     2881 attggaatct gcggatcatg ggaaggcccg gggtgcaggc ggcccatgtt cccatcgccg
     2941 caggggtcgg cctttcgatc aaattccgga agacccggga tgtggtactc tgtttcttcg
     3001 gagacggcgc ctccaacgag gggaacgtgc atgaaggcat gaacctggcc tcggtctgga
     3061 aggccccggt catcttcatc tgcgagaaca acctctatgc tctcttcacc ccgaacgtgg
     3121 agaccacttc ggtcagggat attgccgacc gcgccaaggg atatgcaatg cccggggtga
     3181 tcgtggacgg gaatgatgcc atggctgtgt acgagacggt ccgggaggcc atccagcggg
     3241 cccgttcggg gcagggcccg acctttatcg agtccaagac ctaccggatg cacgggcata
     3301 ccgctatgga ccgctttcac ctgggggggt atcgtccgaa agaagaggtg gaggaatggg
     3361 caaagaagga cccggtcatt ggactgcgaa tccggctgct ggagatgaag gcggcatcgg
     3421 aggcggacct gaaggggatc gaggccgcgg caaaagaaga gatcgaagat gcggagaaat
     3481 tcgccagaga gagcccatat cccaccaaag aagagctgtt ccgggatacc tacgtagaat
     3541 agggaggagg atcggagaat gggatcgaga gagatgacgt atgccgaggc cattcgcgag
     3601 ggcctgcgcg aagaaatgcg gaagaaccaa gagatcatcc tcttgggaga ggatgtcaag
     3661 atcaatgttt ggaccgtgac ccgggacctc tacaaggaat tcggagaccg ggtcatcaat
     3721 acccctctgt ccgaatcggg gttttgcggg gcggcagttg gagcggcctt gaccggcatg
     3781 cgcccggtgg tggagatcat gtatcccgac ttcaccatgt acgccgccga ttccattgcc
     3841 aaccaggcgg ccaaataccg gtacatgtgc gggggaggac ccttcaaggt gccggtcgtc
     3901 ttccgaattg cgggaggcgg gagttccaac ggggccggtt gccatcacgg ccagtccctg
     3961 gaggccacct tcctccattt cccggggttg aaagtggtct ttccatcgac tcctcatgac
     4021 gccaaggggc tgatcaagac agcgatccgg gatgataacc cggttttgtt ctacgaacac
     4081 aagctgctat atggagtcaa aggcccggtt ccggaagaag agtacaccct tcccatggga
     4141 aaggcggagg tgaaacggga agggtcggat gtgaccattg tctcctacgc ttggacattg
     4201 cacaaagtcc ttcgggccgc cgaacaactg gccaaggaag gaatcgaggc ggaggtgatc
     4261 gatttgcgca gcctgcgccc cctggataag gagtgtatcc tccattcttt gaaaaaaacc
     4321 gctaaactag tgaccgccga agaagggacc aaaatgggcg gagtaggggc ggaaatcgcg
     4381 gccgtagtag ccgaagaagg cctccatctg ctggacgccc cggtaaagag attggccatg
     4441 gaggaagtga tcattcccag ctcaaggtac ggggatcaac tgttcattcc ccaggagtca
     4501 gacattgtgg cagcggtaaa ggacctcttc tgatagcgac gtatcccatt tgacaatcgg
     4561 gtaaaccgaa tcctcgatcc gttcatgctt tggcgtgatc catttgagga atgtgccggg
     4621 catttcgggc aaattaaaac aattcgttta aggcatgccg caggagagat aaaaaatggc
     4681 tgtggaggtg aaactgtcga gtctcggggt ttccatcacc gaggggacca tcgtccgctg
     4741 gttgaaaaag gaaggggacc gggtggagaa aggggagatc ctggccgagg tggagacgga
     4801 gaaggtaaat tttgagattg tcgctccgga ggccggagtt ctgctgaaaa tattatacgg
     4861 ggagaagacc atcgccaagg cggaagccac cgtggccctc gtgggtaggg aaggggaaga
     4921 cgtgtcagcc tacttggaaa aagtccgtca ggagagaaaa gaagcgggag aaaaggttgc
     4981 cccttcggaa aagagagaga aagtcgccgg agggaagacg agggcctttc ccgcggcgaa
     5041 attcctggcc aaggaacacg gcgtagacct tgccgaggta accggcacgg gtcccggcgg
     5101 acaggtgacc aaggaggacg tggaaaaatt catcgcccgg aagcagccgc ggcgggagga
     5161 ggggattccc gagctggagg aaatccttcc cctggtagga atccgcaagg ccatcgctga
     5221 cggcatgtcc ctcagcgtcc gcaccgcggc gcacgtcacc accgtggc
//
LOCUS       DASPRR010000065         6584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1951375, whole genome shotgun sequence.
ACCESSION   DASPRR010000065 DASPRR010000000
VERSION     DASPRR010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951375"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VEL68_02300"
     CDS             complement(<1..487)
                     /locus_tag="VEL68_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34832.1"
                     /translation="MRKIEKAIRNPLSALKFLWSIGKGYWYKAKYSILQKDVTIGRNF
                     RVVKGLRIKGPGKVMIGDNVNVDGTTRAVTPYTYSKEAVIRIGSNVFLNGTRFGCGIL
                     ISIGDDCIIGDSSILDTDFHHVDPQKRHSPEPPEAKPIIVERNVWVAGATIILKGVTI
                     GE"
     gene            complement(852..2201)
                     /locus_tag="VEL68_02305"
     CDS             complement(852..2201)
                     /locus_tag="VEL68_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ34833.1"
                     /translation="MNSSRKKSILLVNPWIHDFAAYDLWMKPLGLLYVGSVLRSKGYG
                     ISLLDCLELSSFPDAGLRKPKKKESGKGHFYKEAIPKPGALSSVPRRYRRYGIPPGIV
                     RKLVLQVPRPDLILVTSSMTYWYPGVMETIQLVRENFPGVPIFLGGIYATLCPDHAQR
                     VSGADRVLPGPWDEEKLRVVGEILGDPSQEGEKGFLSWPYPAFDLYPHLPYICLLTRS
                     GCPFSCSYCASHRLARGFESRNPEEVIGELVHWHKKFGVKDFAFYDDALLIQPTAHIL
                     PILKGVMGKKLPCRFHSPNALHVKFIEREVADFLYRGGFKTIRLGFETSNEALQSETG
                     GKVNNQDFRRAVENLKKAGYAGQEIGVYLLAGLPGQRWEEVEDSIAFVKAAGARPILV
                     EYSPIPGTALFEKARRMSPFDLEKEPLFQNNSLLPCRWEGFTWEDFHRLKKNLKEAP"
     gene            complement(2210..3472)
                     /locus_tag="VEL68_02310"
     CDS             complement(2210..3472)
                     /locus_tag="VEL68_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HXZ34834.1"
                     /translation="MKFIADLHLHSKYSRATSQEMEVKTLAQWAKRKGIKLLGTGDFT
                     HPLYFLDLKSKLNPLGNGLFVLKEEPDGTHFMLTVEVSNMYSQGGKSRRIHTLIFAPS
                     FEVVEKINTRLSKYGKIGSDGRPIFGFSAKDLLKMILDLSPECLLIPAHAWTPWFSIF
                     GANSGFDSIQECYEEEYKNIRAIETGLSSDPEMNWRLSSLDDITLISNSDAHSPSKIG
                     REANVFNCELSYRDITEAIRKKDPRRILYTIEFFPEEGKYHYDGHRNCNILFSPQETR
                     KHRSICPVCGKRLTVGVMNRVNELADRPEGSVSPKAIPALHTVPLDEIIADILGVGTN
                     ASSVEKEYLRLVEKGGSEFDILLELSPEQLSSFTPPNILEGILRVREGRLKITPGYDG
                     VFGKIEILSPAERGKEALQAEPKQMGLF"
     gene            complement(3771..4151)
                     /locus_tag="VEL68_02315"
     CDS             complement(3771..4151)
                     /locus_tag="VEL68_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HXZ34835.1"
                     /translation="MITILGEDLDYDKSLAMKMATGMGGGMGRWGTVCGVVTGGAMAL
                     GFCYGRTKAEEKEARDKTYAKVQEMVRQFDKDFGAVQCRDLIHLNLMDPADRKKFDEM
                     GLRKKCSGFVARNVENIRKILKEK"
     gene            4496..4933
                     /locus_tag="VEL68_02320"
     CDS             4496..4933
                     /locus_tag="VEL68_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXZ34836.1"
                     /translation="MDGDNYTIGEFAKMLGLSPRTIDFYTRQGLLHPEQSGRGHGYRH
                     YTEEDRRRVSLIKQLQSKKFSLQEIRQALEISGKEKTVSSAEVREQVTVELERLQAAI
                     EEIRSSASILDQPAVRAVATEALQRATALCSVVVTLLNDMPPF"
     gene            4957..5826
                     /gene="htpX"
                     /locus_tag="VEL68_02325"
     CDS             4957..5826
                     /gene="htpX"
                     /locus_tag="VEL68_02325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HXZ34837.1"
                     /translation="MSNTFKTFILLAGLTALLLLIGQALAGRQGLYFALFMALIMNFV
                     GYWFSDKIALAMSRAQEVSQNQAPDLHQMVERLSVQAGLPKPRVYIIPQDTPNAFATG
                     RNPEHAAVAVTEGILRILKPEELEGVLAHEMAHIKNRDILISSVAAVIAGAISYLATM
                     AQWGAMFGMGRGDDDEGGGGGNMIGTLLMAIVAPMAAMLIQMAISRSREYAADATGAR
                     FVHAGRPLANALLKLEEYNKQIPMQVNPAQAQMYIVNPLSGQSLMRLFSTHPPIEERV
                     ARLMAMRPGDLMK"
     gene            complement(5954..>6584)
                     /locus_tag="VEL68_02330"
     CDS             complement(5954..>6584)
                     /locus_tag="VEL68_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXZ34838.1"
                     /translation="KHGVDFSVHSTTKYIGGHGDVLGGAIICSKENRSKLHEIIKMTG
                     GNLGPFEAWLTHRGIKTLPLRMRQHCFNAGEVARWLESNPRIAKVNYPGLASHPQHEL
                     AKKLFPPGLFGGMISFEIKGAAKEQVFRFMEALEMIIPATTLGDVYSLVLYPPMSSHR
                     ALSPEERAKVGISERLVRLSVGIEEASEIIEDLQKALEKSGRGKSRRKS"
BASE COUNT         1495 a         1815 c         1660 g         1614 t
ORIGIN      
        1 tttcaccaat agttacccct ttaagaataa ttgttgcacc ggcaacccag acattccttt
       61 ccactatgat gggctttgcc tcaggcggct ccggggaatg tcttttttgt gggtctacat
      121 gatgaaagtc agtatccaat atgctcgaat caccgattat gcagtcatct cctattgata
      181 tgagaatgcc gcagccaaac ctcgtcccgt taaggaatac gttgctgcca atacggatta
      241 cagcctcctt tgaatacgtg taaggcgtta cagcacgggt tgtgccatcc acattcacgt
      301 tgtctccaat cattaccttt ccagggccct ttatcctcaa accttttaca accctgaagt
      361 tccggccgat agtcacatct ttttgcagga tactgtattt cgccttatac caatatcctt
      421 ttccaatgga ccaaaggaac ttcaatgcgg agagaggatt tcttatcgct ttttctattt
      481 tcctcacgcg ctggctccct tgatgtttgt cttaatgcac gctattaaag attcttgaag
      541 gcattttggc cgattcgtcc agagaggcct ggccctgcga ttcgcacatc tcccatagga
      601 ggcatctatt tgagacggcg atggtttaaa atcttcatca ggccggtaag gttcatacaa
      661 aagttggata tcttagggcc aagccgctct cttgcaacga gattgttcat aaactcaatc
      721 ggcttctcta ctttgcttcc agtagaccaa taaagcaatc catttgaata tcgtatcatt
      781 ccttattctt ttttcctaga ctcatctgac ccagaaacga agtcatgtca tttgtttatc
      841 atttttggcc tctaaggcgc ttcctttaaa ttcttcttca gacgatgaaa atcttcccag
      901 gtgaatccct cccaccggca ggggaggagg gaattgttct ggaataaggg ctccttttca
      961 agatcgaaag gagacatccg ccgggccttt tcgaataaag ccgtccccgg gatgggggaa
     1021 tattcgacca gaatcggcct ggctccagcc gcttttacaa aagcgatact atcttcgacc
     1081 tcctcccacc tctgccccgg caacccggcc agcagataaa ccccaatctc ctgcccggca
     1141 tatcccgctt ttttcagatt ttccacggcc ctgcggaaat cctggttgtt cactttcccc
     1201 ccggtctcgg actgtagcgc ctcatttgaa gtctcaaagc ccaggcggat ggttttgaaa
     1261 ccacctcgat agagaaaatc agcaacctcc cgttcaatga atttcacatg aagggcgttg
     1321 ggggaatgga agcggcaagg gagctttttc cccatgaccc ccttcagaat cggaaggatg
     1381 tgagcggtgg gctggatgag cagcgcatca tcataaaagg caaagtcctt aaccccgaat
     1441 ttcttgtgcc agtgcaccag ctctccgatc acctcttccg ggtttcggga ctcaaacccc
     1501 ctggccaacc tgtgggaagc gcagtaggag caagaaaaag gacacccgct tcgagtcaag
     1561 aggcaaatat agggcaggtg gggatacagg tcaaatgccg gataaggcca ggagagaaaa
     1621 cctttttccc cttcttggct cgggtctccc agaatttcac ccacgactct caacttctct
     1681 tcgtcccagg gaccgggaag gacccggtcg gcccccgaga ctctttgggc atggtctggg
     1741 cagagggtgg cataaatccc tcccaggaaa atgggcactc cagggaagtt ttcccgtacc
     1801 aattgaatcg tttccatgac ccctggatac cagtaggtca tggaggaagt caccaggatc
     1861 aaatccgggc ggggaacctg gagaacgagt ttcctgacga ttcccggagg aatcccgtat
     1921 cggcgatacc ggcggggaac gctgcttagg gctccgggtt tagggatggc ttctttgtaa
     1981 aagtggccct tgcccgactc ttttttcttc ggtttcctca accccgcatc agggaaagag
     2041 gataattcca gacaatcgag gagagaaatc ccatatcctt tggatcgcag aacgcttccc
     2101 acgtagagca gccccagggg cttcatccag aggtcatagg cagcaaaatc gtgaatccag
     2161 gggtttacga gaaggatgga tttctttctg gaggagttca tgggaggggc tagaagagtc
     2221 ccatctgctt gggttctgcc tggagcgcct ccttccccct ctcggcaggg gataaaattt
     2281 caatcttccc gaataccccg tcgtatcccg gagtgatctt caatcgtcct tcccggaccc
     2341 gcaaaattcc ttccaggatg ttcggcgggg taaaggaaga aagctgctct ggggagagtt
     2401 ccaggaggat atcgaattcc gaaccacctt tctccaccag ccgaaggtat tctttttcca
     2461 ccgatgaagc gttggttccc acgccaagga tgtcggcaat gatttcatca agagggacag
     2521 tgtgaagcgc agggatcgcc ttgggggaaa cagagccctc cggccggtca gccagctcat
     2581 tcacccgatt catcaccccc accgtaagcc gcttcccgca gaccgggcag atgcttctat
     2641 gctttcgggt ttcttggggc gagaaaagga tgttgcagtt tcggtgccca tcatagtgat
     2701 acttcccttc ttccgggaag aactcgatgg tgtaaagaat cctccgcggg tctttcttac
     2761 gaatggcctc ggtgatgtcc cggtaactca gctcacagtt aaagacattg gcctcccggc
     2821 cgattttgct tggggaatgg gcgtcggaat tcgagatcag ggtgatatcg tccagactgg
     2881 agagacgcca attcatttct gggtctgaag agagtccggt ttcgatggcc cggatgttct
     2941 tgtattcttc ctcataacat tcctggatgg agtcaaaacc cgaattggcg ccgaagatgg
     3001 agaaccacgg agtccatgca tgggcgggaa tcaagagaca ctccggcgac aggtccagga
     3061 tcatcttcag gaggtccttg gccgaaaaac cgaagatggg ccggccgtcg gagccgattt
     3121 tgccatattt ggacaagcgg gtgttaattt tttcaacgac ttcgaaggag ggggcgaaaa
     3181 tcagggtatg aatccgacgt gatttcccgc cctggctgta catgttgctc acctccacgg
     3241 tgagcatgaa atgagtcccg tccggctctt ctttcaggac gaaaagccca ttgcccagcg
     3301 gattcaattt ggatttgagg tcaaggaaat agaggggatg ggtaaagtcg ccggtgccta
     3361 aaagcttgat tcccttgcgc ttggcccatt gggcaagggt cttcacttcc atctcctgac
     3421 tggtggcccg gctgtacttg gaatggagat gaagatcggc gatgaatttc atcggaccca
     3481 cttctcagag gactaaaacc cgaaacaaga aatccgaaag gattataacc ctcagaattt
     3541 aacaggatga ggaagaactg aattccggca atcattgcga gggccctaag cgacgcggca
     3601 atctcaaaat gtttgatttt attatgaatg agattgcttc gcttcgcccc gtaccatact
     3661 aggtgcaggg cttcgctcgc aatgacacgc caaagagttt ttcagcatcc tgttaatacc
     3721 caaacaaccg gttgggagaa tttgaaatgc cggaatttcg atatttcgct ttatttctcc
     3781 tttaggatct tgcgaatatt ctccacgttg cgggccacga acccggagca tttcttgcgc
     3841 aggcccattt cgtcgaactt cttccggtcc gccgggtcca tgaggttcag atggatgagg
     3901 tcccggcact ggacggcgcc gaagtccttg tcaaactgcc ggaccatctc ctggactttg
     3961 gcataggttt tgtcccgagc ctccttctcc tccgctttcg tgcggccgta gcagaaaccc
     4021 agagccatcg cccccccggt aaccaccccg cacacggttc cccaccggcc cattccccct
     4081 cccatgccgg tggccatctt catcgctaga gacttgtcgt aatctaggtc ctccccgagg
     4141 atcgtgatca ctgactccgc tcaattcagc ccgctggcca tgaggtcgta agctctcttt
     4201 tccagttccg cccctgattc catcccggtt ccttcccaaa atagatttgc cttcatggat
     4261 tttcgtatta taggggttga gtcgaaactt gtcaaattca ctcttgtttt tccgagtctc
     4321 cggggtttct gtattttttc atcacggtcc ctaaaccatt tgcttttata tagttttttt
     4381 gctcagaaaa gcgcaggcta taatccttga caaacataca gtagagatgt atatttttaa
     4441 taaattaagc cgcagaattc gcaaagcttc attgtgattc acttccaaca aaggaatgga
     4501 cggggacaac tataccatag gagaattcgc aaaaatgtta gggttaagcc cccggaccat
     4561 tgatttttac acccgccagg ggcttctcca ccccgaacag tccggacgcg gccatgggta
     4621 ccgtcactat accgaagagg accgccggcg cgtttcgctg atcaagcagc tgcagtccaa
     4681 gaagttttcc ctgcaggaga ttcggcaggc cctggagata agcggaaaag agaaaacggt
     4741 ctcctccgca gaggttaggg aacaggtcac tgtggagctg gaaaggctcc aggccgccat
     4801 tgaagaaatc cggtcatctg cttcgattct ggatcagcct gctgtccggg ctgttgccac
     4861 agaggccctg caaagagcaa ccgccttatg ctcggttgtg gtgactctat tgaatgatat
     4921 gccgcctttc taggcggccg cgaaaaggag gaaaaaatga gcaatacctt taaaactttt
     4981 attcttctgg ctggacttac ggccttattg ttactgatcg gccaggctct ggcgggacgg
     5041 caaggccttt acttcgccct ctttatggct ctgatcatga acttcgtggg ctactggttc
     5101 agcgacaaaa ttgccctggc catgagtaga gcccaggaag tctctcagaa tcaggcccct
     5161 gacctccatc agatggtgga gcgtctctcc gtacaggccg gcctgcccaa accccgggtc
     5221 tacatcattc cccaggatac ccccaacgcc tttgctaccg gccgcaaccc ggagcatgct
     5281 gctgtggccg tgaccgaggg gattctcaga atcctgaagc ctgaggagtt ggaaggagtc
     5341 cttgcccacg agatggccca catcaaaaac cgggatatcc tcatcagcag cgtcgccgcg
     5401 gtgatcgccg gggccatcag ctacctagcc acaatggccc agtggggggc catgttcggc
     5461 atgggtcggg gtgatgatga cgaaggaggg ggaggcggga acatgattgg gactctgctc
     5521 atggcgatcg tcgcccccat ggctgccatg ctcattcaaa tggccatttc ccgaagccgg
     5581 gagtatgccg ccgatgccac gggggccaga ttcgtccatg ccggacggcc cttggctaat
     5641 gctctcctca agctggagga atacaacaag cagattccca tgcaggtaaa cccggctcag
     5701 gcgcagatgt acattgtcaa tcccctttcg ggtcaaagcc tcatgcgcct cttcagcacc
     5761 cacccgccca tcgaggagcg ggtcgcccgg ttgatggcca tgcggccggg ggacctgatg
     5821 aaataagaag accctttctc atgcaaaaag ccggtctgcc cataagcgga ccggcttttt
     5881 cattccctcc gattattttt tctttacctt tcggtcaaat ccgtaggggt gtttcgcgac
     5941 ccgcccctac gcctcaactc tttctcctgg acttacctct gccgctcttc tccagggcct
     6001 tttgaaggtc ttcgatgatc tccgaagcct cttcgatccc caccgagagg cggaccagac
     6061 gctcgctgat gcccaccttg gccctctcct caggggatag ggcccggtgg gaggacatgg
     6121 gcggatagag gaccaggctg tacacatccc cgagagttgt cgccggaatg atcatctcca
     6181 gcgcttccat gaaacgaaag acctgctctt tcgctgcccc tttgatctca aaagaaatca
     6241 tccccccgaa aagtcccggc ggaaagagtt tcttcgctaa ttcgtgttga ggatgcgagg
     6301 cgagtccggg ataattcacc ttagcgattc gaggattgct ctccagccac cgggccactt
     6361 ccccggcgtt gaaacagtgc tggcgcattc ttaacggcag ggttttgatt ccccggtgcg
     6421 ttagccaggc ttcgaaaggg cctaggttac cgccggtcat cttgatgatc tcgtgcagct
     6481 tggacctgtt ctctttagaa cagatgatcg ctccacccag cacatccccg tggccgccga
     6541 tgtattttgt cgtggaatga accgagaaat ccacaccatg cttc
//
LOCUS       DASPRR010000066        11754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_820163, whole genome shotgun sequence.
ACCESSION   DASPRR010000066 DASPRR010000000
VERSION     DASPRR010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11754)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11754
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820163"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VEL68_02335"
     CDS             complement(<1..1257)
                     /locus_tag="VEL68_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXZ34839.1"
                     /translation="MAAPKGRLNRIRNIGIMAHIDAGKTTVTERILYYSGRSHKMGEV
                     HDGEATMDWMRQEQERGITITSAVTTFPWRGQEIHLIDTPGHVDFTIEVERSLRVLDG
                     AIAVFDAVNGVEPQSETVWHQADKYQVPRLTFINKMDRVGADFFSSVESMKEKLGANP
                     VLIQLPLGAEDRFQGVIDLIEMKSIIWDEETLGATFQEMDIPSELMPDAGKYREKLLE
                     TLAEKSDPLMEKYLAGEEVGTEEIHQVLREGTIKFNLVPVLCGAALRNKGIQPLLDAV
                     VNYLPSPLEVPPVQGFNPQTGDPERRSSSDQEPFSALAFKIMTDEGRKLTYLRVYSGV
                     LKAEQEIYNSTKGKRERVARLFRMHANKKERVPEVRAGDIVGAAGLKETTTGDTLCAE
                     DHPVTLERIDFYEPVTSVAIEPKSRQD"
     gene            1561..2745
                     /locus_tag="VEL68_02340"
     CDS             1561..2745
                     /locus_tag="VEL68_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34840.1"
                     /translation="MGKKTLNGLLLSLAAVLLIAFPGTALAQDTLKIGFFAPITGPAA
                     VDGTSAKNAVEIGLKEVNDAGGIRGKKVELIIYDDRMKAEEAVAIANKLIEKDKVIGV
                     VSGSYSAPTRVTAPIFQKAGMPMVAGYAVHPDVTWDPKAKKPNDFIFRNGFLGEIEGA
                     AAAEFAVKNLKAKKVSLIFMDNDFGRAISAGFAAHAEKLGASILTKQMYKFPGEKDFR
                     PFLTRIKEGDPDLIFAAGYYNEAAAIVRQSKELGIKSAIMGEEGFDSPKFLEIAGPAA
                     EGVIIATNLDRDDPRPLVQNFLKSYRQAYGHDADMVGASSYDAFKILVAAVEKAGTDP
                     KAIQQALLDTKDYNGLTGKITRFVQGEVIKPVQVQVVKEGKFRRLGVVDNPEIITPPI
                     KK"
     gene            2852..3709
                     /locus_tag="VEL68_02345"
     CDS             2852..3709
                     /locus_tag="VEL68_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34841.1"
                     /translation="MLWQQLANGISVGSVYALISLGLTMVYGILRILHVAHAGIYTLG
                     AYVGLLAFRLIGDFWLSLLVGMAGSAVAGYLVERLLYMPLLHLPRIAPLIVSIGLFTA
                     MEEGFRIVAGPHTLTYNADIGLGSVSLGSFQLTETQVLILLVTFAILVFLWAFLTKTR
                     MGLAMRACAMDAETTSSFGANVTAIIAMNFLLGSAMAGAAGVLIGVYDNQVWPTMGSI
                     PAYKALAIVVLGGLGNIPGTVLASLIVGLAETFFLGILAIPFPRDAVAFIAMILVLMF
                     KPYGLLGKA"
     gene            4018..4911
                     /locus_tag="VEL68_02350"
     CDS             4018..4911
                     /locus_tag="VEL68_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34842.1"
                     /translation="MISGYVITILITVAIYAILAHSLNIITGHAGQISLGHAAFFGIG
                     AYTSALLYTEASLPFWLSVPLAAVTAGIIGAVLGIPCLRVRDDFLAITTMGINFVVEA
                     VFLYIPFFGGAMGIGGINLPDWFGREMSKPEYFILILVVFGFLLLVDYGLGRSWIGLA
                     WASIREDEQAAEAMGVDVVRFKVLAFILGSMIAGLAGGFYAHFLTFIMPQNFGFPMSI
                     VILSMVVFGGIGTRWGPLVGALILGVLPELSRPIMEYRTFLYGLLLMFMMRFQPDGLL
                     GSESVLMKGIRRLVPRPSLSR"
     gene            4916..5662
                     /locus_tag="VEL68_02355"
     CDS             4916..5662
                     /locus_tag="VEL68_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34843.1"
                     /translation="MSLLQVTSLGKEFDGLRALDGVSFAAEPAQVLGIIGPNGAGKTT
                     LFNLISGVYPPSSGEIRFQGESILKLKPHEISRKGIGRTFQIVRPFQNLDVETNVLVA
                     LGQRFYPSFWQSTRAYRQPEVLQEARTLIQEAGIRSFARHQAKNLPLGIKKRLEIARA
                     LALRPRLLLLDEPSAGLPYEESTQLMDLIRKVCAQGIGVLLIEHNMHVVMDLCRRIVV
                     LDHGSRIAEGTPEEVRSNPLVIQAYLGKET"
     gene            5662..6369
                     /locus_tag="VEL68_02360"
     CDS             5662..6369
                     /locus_tag="VEL68_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34844.1"
                     /translation="MLEVRDIEVAYGEILALKGISFTVKEGELVTIIGANGAGKTTIL
                     RAVMGLARCRKGAILFRGKDITQSPPHDRARLGISPVPEGRRVFPDLTVAENLLMGAY
                     ALEDRKATSDRMEQAFSLFPILRERQAQVGKTLSGGEQQMLAIARALMAQPRVILMDE
                     VSLGLMPIYVEETFRVIRDLHRNGVTILLVEQNARKALAAADRGYVLETGQIVLSGPS
                     QELSTNPQVKKAYLGGT"
     gene            6528..8177
                     /gene="hutU"
                     /locus_tag="VEL68_02365"
     CDS             6528..8177
                     /gene="hutU"
                     /locus_tag="VEL68_02365"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXZ34845.1"
                     /translation="MKVNISAPRGTKITCKSWQTEAALRMLMNNLDQEVAEKPDELIV
                     YGGAGKAARNWDCYLKIVECLKNLGNDETLLVQSGKPVGIFRTHTFAPRVLISNSMLV
                     PKWATREKFQELEALGLTMYGQMTAGSWIYIGSQGILQGTFETFAAAARQHFDGSLKG
                     RLVLTAGLGGMGGAQPLAATMNGAVFIGVEVDPERIKKRLETKYLDTMTDNLDEALRW
                     AFEAKEKGIPKSIGLVGNAAEVHPEMVRRGIVPELVTDQTSAHDPLRGYVPAGMTLAA
                     ALELREKDPEEYVKQAMKSMAIHVRAMLDFKRRGSIVFDYGNNLRAQAFEEGVKDAFD
                     FPGFVPAFIRPLFCEGKGPFRWAALSGDPEDIYKTDEAVLREFPENESLRRWMKLARE
                     RVKFQGLPARICWLGYGERARIGKVFNDMVASGTLKAPIVIGRDHLDAGSVASPNRET
                     EAMMDKSDPIADWPILNALLNTAAGATWVSVHHGGGVGIGYSIHAGMVMVCDGTKEAE
                     ERILRVLTTDPGTGIMRHVDAGYDIAVDAAKKFGIKIPMMK"
     gene            8439..9332
                     /gene="ftcD"
                     /locus_tag="VEL68_02370"
     CDS             8439..9332
                     /gene="ftcD"
                     /locus_tag="VEL68_02370"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HXZ34846.1"
                     /translation="MKILECVPNFSEGRDDKKVEQIVGEVRRTSGAKLLDYFSDQDHN
                     RTVVTFLGEPAAVREAALKASLKALELIDMRTHKGGHPRIGAVDVVPFIPIRGMDMSE
                     TAHIAREMGRELGEAGQIPIYFYGEAATDPKRKKLADIRKGEYETLKEKLATPGWGPD
                     AGLSTFNPKSGATVVGARMPLIAFNVNLRPPKLDQAKALARKVRESSGGIPSVQALGL
                     ELKEKAMVQVSMNLTDYRRASISTVVEAIRREIAGKGLEIAECEVVGLVPMEALVDVL
                     REYLKMPEFDPKQIIETHLLE"
     gene            9427..10074
                     /locus_tag="VEL68_02375"
     CDS             9427..10074
                     /locus_tag="VEL68_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HXZ34847.1"
                     /translation="MAQEDQKDDVTLSQFLDAVAGPTATPGGGSVSALAGTLAASLVE
                     MVVNLTVGKRGFEAQEAEMKKLGADAHSYREALGSTIAQDIDAYQAVMKAYLLPKSTE
                     TEKGIRKEEIQKALRRAADPPLFTAATSLKVLKLCQKAAEMGNPSTITDAAVGALLAE
                     AALQGGVLNVLINLSALEDENTVKKMMRELKRLETEGIKTRERILNQVKGRLKAE"
     gene            10071..11369
                     /gene="hutI"
                     /locus_tag="VEL68_02380"
     CDS             10071..11369
                     /gene="hutI"
                     /locus_tag="VEL68_02380"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXZ34848.1"
                     /translation="MKRGADFLVENAAQLATITFSPRATNPEEKLGVIRNGTLAAKGG
                     RIVWVGPAGDTSKQVSLLPGGKEMDASGKVVLPGFIDSHTHLIFAGTREKEFELRLQG
                     ASYQEIAAKGGGIKSTVEKTRRASKDELVQIGKKNLDSMLSLGTTTVEAKTGYGLSTK
                     DEIKMLEALRDLSREHPIELVPTFLGAHEIPPEYAGKTEDYVRLVIDEMIPAVAEKEL
                     AIFCDVFCEKGVFSLEQSRRILEAGKRYGMIPKLHADELTPLGGAELAAEIGAVSADH
                     LLFISDKGMQAMAQKGVVATLLPGTAFFLFLGRYAPARQMISKGVNVALASDFNPGSC
                     MTESLPLITTIACTQMRMTPAEAIQGITLNAAKALRKEKEIGSLEPGKQADLVVLDIP
                     DYRHLSYHFGVNHVWKVVKRGRIVWEAESSHSTIRVDPRH"
     gene            11584..11724
                     /locus_tag="VEL68_02385"
     CDS             11584..11724
                     /locus_tag="VEL68_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34849.1"
                     /translation="MESAAILILALVTLALPFLPKLCRKKTAKKSSELFSVEAPVADG
                     VG"
BASE COUNT         2567 a         3332 c         3401 g         2454 t
ORIGIN      
        1 atcctgccgg gatttcggtt cgatggccac cgaggttacc ggctcgtaaa aatcgattcg
       61 ctccagggtg acgggatggt cttcggcgca gagagtgtct ccggtggtgg tctccttcag
      121 accggcggcc ccgacgatat ccccggccct cacctccgga accctctcct ttttattggc
      181 atgcatccgg aaaaggcgag cgacccgttc acgcttgccc ttggtggaat tgtaaatttc
      241 ctgttcggcc ttgaggaccc cggaatagac ccgaaggtag gttagcttcc tcccctcgtc
      301 ggtcatgatc ttgaaagcca gggcggaaaa gggctcttga tcggaactgc ttcgcctttc
      361 cgggtctcct gtttgggggt tgaatccttg gaccggaggg acctccaagg gtgaaggcag
      421 gtaattcacc accgcatcca ggagaggctg aattccctta ttgcgtaagg cagctccgca
      481 aaggacggga accaggttga attttatcgt gccctctcgc agaacctggt gaatctcctc
      541 ggtaccgacc tcttccccgg ccaggtactt ttccatgagg ggatcgctct tctcggccag
      601 ggtttccaga agtttctccc ggtattttcc ggcatcgggc atgagctcag aggggatatc
      661 catctcctga aacgtagccc ccagggtttc ttcgtcccag atgatggact tcatctcgat
      721 aaggtcgata accccctgaa accgatcttc ggctcccagg ggcaactgga tcagaaccgg
      781 attagcaccc aatttttctt tcatgctctc cacggagcta aagaaatcgg cccctacccg
      841 gtccattttg ttaatgaagg tgagacgggg aacctggtac ttatcggcct ggtgccaaac
      901 cgtctccgac tggggttcaa ctccattgac tgcgtcgaag acggcaatgg ccccgtccag
      961 aacccgcagg cttctttcca cttcgatggt gaaatccaca tgaccggggg tatcgatcag
     1021 atgaatttct tgccctcgcc atggaaaagt cgtcaccgcg gaggtaatgg taattccccg
     1081 ctcctgttcc tggcgcatcc agtccatggt agcctccccg tcatggactt cgcccatttt
     1141 gtgggaacgg ccactgtaat aaaggatgcg ttcggtgacc gtagtcttgc cggcatcgat
     1201 atgagccatg atgccgatat tacggattct gtttaggcga cctttgggtg cggccataac
     1261 ctttagccat cactctccgc gggcggcttt catccgactc ttggggcatg cccaaagccc
     1321 gcgaatttat ctccaaaacc gctcgttctt ggaaaatccc cttaaataaa caataatagg
     1381 gaatttctcc cctcatgtca atataaaacc atgaaaggat taaggaaaac atgcgaagaa
     1441 ggaggagttc ccggtgcatg gtcctcatcc ttcagaagtt tcccccttga cccctctttg
     1501 ttcttcttat aaattcagat tgacttattt ctcttcactt ctaggcaagg aggaatggat
     1561 atgggaaaga aaaccctgaa tggactgtta ttaagcttgg ccgcggttct tcttatcgct
     1621 ttccctggaa ctgccctggc ccaggatacc ctcaagatcg gcttcttcgc ccccatcacc
     1681 ggtccagcgg cggtggatgg cacaagcgcc aagaacgccg tggaaatcgg cctaaaggaa
     1741 gtgaacgatg ccgggggaat tagagggaag aaagttgaat tgatcatcta tgacgaccgg
     1801 atgaaggccg aggaagcggt ggccatcgcc aataaattga tcgagaaaga taaagtcatc
     1861 ggcgtggtca gcggttccta cagcgcgccc acccgggtca ccgcccctat cttccagaag
     1921 gcgggtatgc ccatggtggc cggttatgcc gtccacccgg acgtgacctg ggaccccaag
     1981 gcgaaaaagc ccaatgactt catcttccgc aacggctttc tgggagagat cgaaggggcg
     2041 gcggccgccg aattcgcggt aaagaacctg aaggccaaga aggtgtcctt gatcttcatg
     2101 gacaatgact tcggccgggc catatccgcc gggttcgcgg cccacgccga gaagctgggg
     2161 gcttccatcc tgaccaagca gatgtacaag ttcccggggg agaaggactt ccgtcccttc
     2221 ctgacccgga tcaaggaagg agatcccgac ctgatcttcg ccgcggggta ttacaacgag
     2281 gctgcggcca tcgtccgcca gtccaaggaa ctaggaatca agagcgcgat catgggggag
     2341 gaaggattcg attctcccaa gttcctggag atcgccggcc ctgcggcgga aggggtcatc
     2401 atcgctacca acctggaccg ggatgacccg cggccgctgg tccagaattt cctgaagagc
     2461 taccgccagg cctacggcca tgatgccgac atggtagggg cttccagtta cgacgcattc
     2521 aaaatcttgg tggcggccgt cgagaaagcc ggaaccgatc cgaaagcgat ccagcaagcc
     2581 cttctggata ccaaggacta caacggcctt acaggaaaga tcacccgctt tgtacaggga
     2641 gaggtgatca aaccggtgca ggtccaagtg gttaaagaag gaaagttccg ccgcctgggg
     2701 gtagtggaca acccggaaat catcacaccg ccaataaaaa aatagcggtc agcattcagc
     2761 tttcagccat cagctaaaac caagacgctg atcgcaaatg gggaagcggg gtctgctgat
     2821 tgctgatcgc tgatcgctga aagctgcctt tatgctctgg caacagttag ccaacggaat
     2881 ctccgtcgga agcgtctacg ccctcatctc cctcgggttg accatggttt acgggatcct
     2941 gcggatcctg catgtcgctc atgcgggaat ttacaccctg ggcgcttacg tgggactcct
     3001 cgccttccga ctcatcggcg atttttggct ttccctgctg gtgggaatgg ccggttcggc
     3061 ggtggctgga tacctggtgg agcggctcct gtatatgccg ctgctccatc tgccccgcat
     3121 tgccccgctc attgtgtcca tcgggctgtt tactgctatg gaggagggat tccgcatcgt
     3181 cgccggccct cataccctca cctataacgc cgacattggg ctgggaagcg tctccctggg
     3241 aagctttcaa ctcacggaga cccaggtctt gattctcctg gtcactttcg cgattctggt
     3301 ctttctctgg gcctttctca cgaagacccg catgggcctg gccatgcggg cctgcgccat
     3361 ggatgcggag accacttcat ccttcggggc caatgtgacc gccatcatcg ccatgaattt
     3421 tctactcggc tcagccatgg ccggagccgc gggggtcctc atcggggttt acgacaacca
     3481 ggtctggccg acgatgggaa gcattcccgc atataaagct ttggccatcg tcgtcctggg
     3541 aggtctcggg aacatcccgg gaacagtttt ggcctccctc atcgtcggcc tggccgagac
     3601 cttcttcctt ggcatcctgg ccattccctt cccccgagat gcagtggcct ttatcgccat
     3661 gattctggtt ttaatgttta agccgtatgg attgctgggc aaagcctaaa atagcgtgca
     3721 gcggtcagct ttcagccccc ccccacccta tccgttcggc tgagctcacg acgaagccct
     3781 ccccctcaag agactgtgtc gcaattgggg aaaggagtcg tcattccggc gaaagctcgt
     3841 agagtcgagt ctccgaccgg aatccagttt ttgattttat tcaatttttc gatatcctgg
     3901 atgccagctt tcgctggcat gacgaattac gacacagtct ccaagggggg agggttgggg
     3961 gagggagtga aggaatgaag gctgacagct gaaagctgtt agctggaagc tgtatttatg
     4021 atcagcggtt acgtcatcac cattctcata accgtggcca tatacgcaat tttggcccac
     4081 agcctgaata tcatcaccgg gcacgcgggg cagatctccc tgggccatgc ggcctttttc
     4141 gggatcgggg cttacacttc cgccctgttg tacaccgaag ccagcctccc cttctggctc
     4201 agcgtccctc tggcagccgt caccgccgga atcatcgggg ccgtcctggg cataccctgc
     4261 ctccgggtac gggatgattt tctggcgatc actactatgg ggatcaactt cgtggtggag
     4321 gccgtcttcc tctacattcc tttcttcggt ggtgccatgg gaatcggagg gatcaatctc
     4381 cccgattggt tcgggaggga gatgagcaaa cccgaatatt tcatcctgat tctagtggtc
     4441 ttcgggtttc tgctgctcgt ggattacggg ctggggcggt cctggatcgg cctggcctgg
     4501 gcttccatcc gggaggacga gcaggcggca gaagccatgg gcgtggacgt ggttcggttt
     4561 aaggtcctgg cctttatcct gggaagcatg attgcgggtc tggcgggcgg gttctacgcc
     4621 catttcctga ctttcatcat gccccagaac ttcggcttcc ccatgtccat cgtgattctc
     4681 tccatggtgg tcttcggggg gatcgggacc cgctggggac cgcttgtggg agcgttgatc
     4741 ctgggcgttc tccccgagct ctcccggccc atcatggagt accgaacgtt tctctacgga
     4801 ctcctgctca tgttcatgat gcgctttcag cccgacggcc ttctgggttc cgagtcagtc
     4861 ttgatgaagg ggatccggcg gttggttcca cggccctccc tctcaaggtg attctatgtc
     4921 tctgttgcag gtaacttctc tgggaaagga atttgacggg ttgcgcgccc tggacggggt
     4981 cagttttgcc gctgagccgg cccaggtcct ggggatcatc ggcccgaacg gggccgggaa
     5041 aacgactctc ttcaatctca tctcaggggt ctacccgccc agttccggag aaattcgttt
     5101 ccagggggag tctatcctca aattgaagcc ccacgagata agtcggaagg gcattggccg
     5161 gaccttccag atcgtccggc ctttccagaa cctcgacgtg gaaaccaatg tgctggtggc
     5221 cctgggccag cggttctatc cctctttctg gcagtccacc cgggcctacc gccagccgga
     5281 ggtcctccag gaggccagga ccttgattca ggaagccggg atccgatcct ttgccaggca
     5341 tcaggccaag aaccttcccc tggggatcaa gaagcgcctg gaaatcgccc gtgccctggc
     5401 tctgcgcccc cgcctgctcc ttctggatga gccgtccgca gggttaccgt acgaagaatc
     5461 cacccaactc atggacctga tccgcaaagt ttgcgcccaa gggatcggtg ttctcctgat
     5521 cgaacacaac atgcacgtgg tgatggacct atgccggcga atcgtggtcc tggatcacgg
     5581 gagcaggatc gccgagggga ccccggagga ggtccgctcg aaccccctgg tcatccaggc
     5641 ctacctggga aaggaaactt gatgctggaa gtccgggaca tcgaagttgc ctacggagaa
     5701 atcctggctc tcaaagggat cagctttacg gtgaaagaag gagaactggt taccattatt
     5761 ggggccaacg gagcgggcaa aaccacgatt ctccgggcgg tcatgggctt ggccagatgc
     5821 aggaaagggg ccattctctt ccgggggaaa gacatcaccc aatctcctcc ccatgatcgg
     5881 gctcgtcttg gaatcagtcc cgttcccgag ggccggaggg tctttcccga ccttacggtg
     5941 gcggaaaatt tgcttatggg cgcctacgcg ctggaggatc gaaaggcaac ctctgaccga
     6001 atggagcagg ccttcagcct ttttcccatc ctgcgggaaa gacaggccca ggtggggaaa
     6061 acgctcagtg gaggagagca gcagatgctg gccattgccc gggcgctgat ggctcaaccc
     6121 cgggtcatcc tgatggatga ggtctccctg ggccttatgc cgatttacgt ggaagaaacc
     6181 tttcgggtca tccgggacct tcatcggaat ggggtcacga ttcttctggt ggagcaaaac
     6241 gcccggaaag ccctggccgc tgcggaccga gggtacgtac tggaaaccgg gcaaatcgtg
     6301 ctctccggtc ccagccagga gctttccacg aaccctcagg tcaagaaagc ctacctggga
     6361 ggaacgtaaa agaagagtgc ccaaaatgcc caaagtgcgc taaagtgcct aatgttggaa
     6421 aatccaagaa caagaaaccc gaaccaaatt tattaggagt atggaacatg aaaagaacca
     6481 gaccggtaaa agtcatggag aagcccaagg tgaaaaccca ggtcggcctg aaggtgaaca
     6541 tctcggctcc ccggggaacc aagatcacct gcaagagctg gcagacggaa gctgcgttgc
     6601 ggatgctcat gaacaacctg gaccaggagg tggccgagaa gcccgatgag cttatcgttt
     6661 acggaggagc cggcaaagcc gcccggaatt gggattgcta cttgaaaatc gtcgagtgcc
     6721 tgaagaacct ggggaatgat gagacccttc tcgtgcagtc ggggaaaccc gtgggaatct
     6781 ttcgaactca tacctttgct ccccgggtcc tcatctccaa ctccatgctg gtgcccaagt
     6841 gggccacccg ggaaaagttc caggaactag aagccctggg cctgaccatg tacgggcaga
     6901 tgactgccgg aagctggatc tatatcggct cccagggaat tctccagggg acttttgaga
     6961 cctttgccgc cgctgcccgg cagcatttcg atggttcgct gaaaggccgg ttggttctca
     7021 ccgcgggcct cgggggcatg ggcggggccc agcccctggc ggcgacgatg aatggcgctg
     7081 tttttatcgg tgtggaagtg gacccggaac ggatcaagaa gcgcctggag acaaaatacc
     7141 tcgacaccat gaccgacaac cttgacgaag cccttcgttg ggctttcgag gccaaggaga
     7201 aaggaattcc caagtccatc ggtctggtgg ggaatgccgc cgaggtgcat ccggagatgg
     7261 tgcgcagggg aattgttccc gaattggtta ccgaccagac ttcggcccat gatcccctta
     7321 gaggctacgt ccccgccgga atgaccctgg ccgcagctct tgagttgcgg gagaaggacc
     7381 cggaagaata cgtgaagcag gccatgaaat ccatggccat tcatgtgcgg gccatgctcg
     7441 acttcaagag gcggggttcc atcgtttttg attacggaaa caacctccgc gcccaggctt
     7501 ttgaagaagg ggtcaaggat gcttttgact tccccgggtt tgtgccggcc ttcatccgtc
     7561 ccctcttctg cgagggcaag ggacctttcc gctgggctgc cctctccgga gaccctgagg
     7621 acatctacaa gactgacgaa gcggtcctgc gcgaattccc ggagaacgag tctctccggc
     7681 ggtggatgaa actggcccgg gaaagggtga agttccaggg gctgcccgcc cgcatctgct
     7741 ggctgggcta cggggaaagg gcgcgaatcg gcaaggtctt caacgacatg gtggccagcg
     7801 gaacactcaa ggcccccatc gtcattggac gggaccatct cgacgccggg tccgtcgcct
     7861 cccccaacag ggagacggaa gccatgatgg acaagtccga ccccatcgcc gactggccca
     7921 tcctcaacgc cctgctcaac accgccgccg gggccacctg ggtctccgtc caccacggcg
     7981 ggggggtggg gatcgggtac tccatccatg ccggaatggt tatggtttgc gacgggacca
     8041 aagaagccga ggaaagaatc ctccgcgtcc tcacaacgga ccctggcacc gggatcatgc
     8101 gccatgtgga tgcgggttat gatatcgccg tggatgcggc caaaaaattc gggatcaaaa
     8161 tacccatgat gaaatagcca tttttcacca cagagacaca gagggcacag aggtgaaatg
     8221 atggcaaagg ggaaagataa ctggtcataa agaaaggagt cattgcgaac gaagccctgc
     8281 acctagtatg acgcagggcg aagtgaaggt cgatccgacc tgcgtcgctt cgctcctcgc
     8341 aatgattgcc atgttaaact tttccctata aatcaagaac aaactaaatt ttccgttttc
     8401 ttggtgtcct ctgtgcctcc gtggtgaaag gagctttcat gaaaatcctc gaatgcgtcc
     8461 ccaacttcag cgaagggaga gatgacaaga aggtcgagca gattgtgggt gaggttcgaa
     8521 ggacttccgg ggccaaactc ctcgactatt tctccgacca ggatcacaac cgtacggtag
     8581 tgactttcct gggagagcca gctgccgtcc gagaagccgc cctcaaggct tccctcaaag
     8641 ctctggaact cattgacatg cggacccata aggggggcca cccccgcatc ggagcggtcg
     8701 atgtggttcc tttcattccc atacggggaa tggatatgag cgagacagcc catatcgccc
     8761 gggagatggg aagagagctg ggcgaagccg gtcagattcc gatttacttt tacggagagg
     8821 cagcgaccga tcccaaacga aaaaaactgg ccgacatccg caagggggag tacgaaaccc
     8881 tcaaagaaaa actggccact cccggatggg ggcccgatgc gggtttatcc actttcaacc
     8941 ccaaatccgg ggccaccgtc gtgggagccc gcatgcctct catcgccttc aacgtcaacc
     9001 ttcgcccccc caagcttgac caggccaagg cgttggcccg caaggtccgt gagtccagcg
     9061 gggggatccc gtcggttcaa gcattgggcc tggagttgaa agaaaaggct atggttcagg
     9121 tttccatgaa cctgaccgac taccgccggg cttccatatc tacggtggtg gaggcgatcc
     9181 gcagggagat cgcaggcaag gggctggaga tcgccgaatg cgaggttgtg ggactggtcc
     9241 ccatggaagc gttggtcgat gtgttgcggg aatacctgaa gatgcccgaa ttcgacccga
     9301 aacagattat tgaaacgcac ctgctagaat agcggtcagc attcagctat tagctgtcag
     9361 cttaaccccc caaacgtctt ggctgaccgc tgaaagctga cagctgatcg ctttttagga
     9421 gaccgaatgg cccaagaaga tcagaaagac gatgtgacgc tgtcccagtt tctcgacgcc
     9481 gtggccggac cgaccgccac tcccggcgga gggagcgtct ccgctctggc cgggaccctg
     9541 gccgcgtctc tggtggaaat ggtggtaaac cttaccgtgg ggaagagggg ctttgaggcc
     9601 caagaggccg agatgaaaaa gttgggggca gatgcccact cctaccggga ggccctgggt
     9661 tccactattg ctcaggacat cgacgcctat caggcggtca tgaaggccta ccttctcccc
     9721 aaatctaccg aaaccgagaa aggcatacga aaagaggaga tccagaaggc cctgcgaagg
     9781 gcggccgacc ctcccctctt taccgcagcc accagcctga aggtcttgaa gctctgccag
     9841 aaagcggcgg aaatggggaa ccccagcacc atcaccgatg ctgcggtggg ggccctcctg
     9901 gccgaagcgg ccctgcaggg cggggtctta aatgttctca tcaacctctc cgccctggag
     9961 gatgagaata cggtgaagaa gatgatgcgg gagctcaagc gtctggagac ggaggggatc
    10021 aagacccggg agaggatttt gaaccaggtg aaaggaaggc tgaaagccga atgaaacggg
    10081 gagcagattt cctggtggaa aatgccgccc aactagcgac catcactttc tccccccggg
    10141 cgactaaccc ggaggaaaag ctgggggtga tccggaacgg gactcttgcc gcaaaaggag
    10201 ggcggatcgt ctgggtgggg ccggcagggg acacttccaa acaggtttct ctccttcccg
    10261 gggggaaaga gatggacgct tccgggaaag ttgtcctgcc ggggttcatc gactcccaca
    10321 cccaccttat tttcgccggg acccgggaaa aggaattcga gcttcgcctc cagggagcct
    10381 cttaccagga gattgcggcc aaagggggag ggatcaaatc gacggtggag aagacccgca
    10441 gggcgagcaa agacgaactc gtccagatcg ggaaaaagaa cctagactcc atgctttccc
    10501 tgggcacgac caccgtggag gccaagaccg gctacggcct ttccacgaaa gacgaaatca
    10561 agatgctcga agccctgcgg gatttgagtc gggaacatcc gatagagtta gtgccgactt
    10621 ttttgggcgc ccatgagatt cccccggaat acgccggaaa aacggaagat tacgtccgcc
    10681 tggttatcga tgagatgatc ccggcggtgg cagagaaaga actcgcgatc ttctgcgatg
    10741 tcttctgcga gaagggtgtc ttctcactgg agcagtctcg gcggattctg gaggcgggga
    10801 aacgttatgg aatgatcccc aagctccatg ccgatgaact gaccccgctg ggcggcgcgg
    10861 aattggccgc cgaaatcggc gccgtttccg ccgatcatct tctctttatc agcgataagg
    10921 gcatgcaagc catggcccaa aaaggggttg tggccaccct tcttcccgga accgctttct
    10981 ttctttttct gggccgctat gctccagccc gccaaatgat ctccaagggg gtcaacgtcg
    11041 ccttggcctc cgacttcaac ccgggctcct gcatgaccga atccctcccc ctgattacta
    11101 ccatcgcctg cacccagatg cgcatgaccc cggctgaagc catccagggc attaccctca
    11161 acgccgccaa agccctgaga aaggaaaagg agatcggcag cctcgagccc ggcaagcagg
    11221 cggacctggt ggtcctggac atccccgatt accgccacct gtcctaccac ttcggcgtga
    11281 atcacgtctg gaaggtcgtt aaaaggggcc ggatcgtctg ggaggccgaa tcttcccatt
    11341 ccacgatccg cgttgatccg cgtcactaat tgggccaaaa ttgcatcgac gcttaagact
    11401 tagccgttcg tgctgaggcc gtcgaagcat gaacggcata gagatgcacc cttcgacaag
    11461 ctcagggtga acggtgacag ctcagtgaaa agaccggggt tagaagagat tgtcggaaga
    11521 atatgtcttc tcaattgtgg actcccccgt agttcacctc tcggggcaag gatttgggtc
    11581 tgtatggaat ccgctgccat cctgatcctc gccctggtta ctcttgccct cccctttttg
    11641 cccaagctct gccggaaaaa gaccgcgaag aaatcctctg aacttttttc agtggaagcg
    11701 cccgtggcag atggagtagg atagggtgaa aagaccgctc cttcggaaag agaa
//
LOCUS       DASPRR010000067         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2114070, whole genome shotgun sequence.
ACCESSION   DASPRR010000067 DASPRR010000000
VERSION     DASPRR010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114070"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VEL68_02390"
     CDS             <1..122
                     /locus_tag="VEL68_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HXZ34850.1"
                     /translation="LKGVTIGENSVVGAGSVVTADIPRNHLALGNPAQVIKKI"
     gene            337..753
                     /locus_tag="VEL68_02395"
     CDS             337..753
                     /locus_tag="VEL68_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXZ34851.1"
                     /translation="MVLSDAISLSTKGFCDIQDITSPVSAIVGKSRVQNGTVTIFCKG
                     STGAVTTIEYEGGVLEDLKKAIERIAPSNIPYAHDQRWGDGNGFSHVRAAILGPSLTV
                     PIFQSSLALGTWQQIVFIDFDNRSRRRNIMVQVMGE"
     gene            complement(1020..1541)
                     /locus_tag="VEL68_02400"
     CDS             complement(1020..1541)
                     /locus_tag="VEL68_02400"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXZ34852.1"
                     /translation="MVSEKASIKAKNDRDDLTGEHAFGDAGQIILACLFIGVWIADTF
                     FFQYTTFLNRYIHLGARIPLGVLLFILSAYLARTGLAIVFGERREKPAVIRKGVFGVV
                     RHPVYLSEILFYLALVMLSLSLAAAGVWGVTIVFLHYISKYEEKLLLARFGDDYAKYM
                     QEVPMFLPRFRKR"
     gene            2003..3496
                     /gene="thrC"
                     /locus_tag="VEL68_02405"
     CDS             2003..3496
                     /gene="thrC"
                     /locus_tag="VEL68_02405"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXZ34853.1"
                     /translation="MEYISTRGSAPAIPSKKTIIKGIAEDNGLYIPSTFPSFGPSPFV
                     GVQRKSYSERAVKILSPFLSDYTDEELNTACGDAYAKSFESPQIAPLRILGDGTAVLE
                     LWHGPTQAFKDMALQLMPRLLSFALKGEKEKYEVVILVATSGDTGKAALEGFADAEGV
                     RIFVFYPHGGVSEIQRLQMVTQQGKNVGACAVKGNFDDAQTGVKQIFSNSALNRKLAD
                     FHFQLSSANSINWGRLAPQVAYYFTGYDDMVAAGKVRQGQPILICVPTGNFGNILAAY
                     YAIRCGLPVSRLICASNRNKVLTEFIRTGVYDRRREFFKTESPSMDILISSNLERLLY
                     ELSGRKANVVVQWMKDLSIQGRYKIDQRGLEELQKFFYGGFADLRQTLAAIRRAFEKQ
                     GYLMDTHTAVGYSVLQEYREETGDGTPALLASTASPFKFNQSVLEALGRDTSGKDEFT
                     LLEELSAMSGQPVPVKLTELKSLPELHGGVCEREGMAQALFQFLKVP"
BASE COUNT          875 a          946 c          966 g          815 t
ORIGIN      
        1 ttcttaaagg ggtaactatt ggtgaaaatt cagttgttgg tgctgggtcc gtcgttactg
       61 ccgacatccc caggaaccat cttgcccttg gaaatccggc tcaggtaata aagaagatat
      121 gagcgaatac aacttaagaa atcccggatt ccagatttgt ccacctcctt tctatcccca
      181 gcatcattca tgcgaactca gcggcgtcca aattaggtag ggaaatcctg ttctcccggg
      241 gagggcggga ggaatctccg aaagatcttt tcaaggcccg cagaattgat acgataggta
      301 tattttcccc gatgggaaat agccaggagg taacccatgg ttttgtccga cgccatttct
      361 ttatctacca aagggttttg cgatatccag gacatcactt ctccggtatc cgccatcgtg
      421 ggcaagtcca gggttcagaa cgggacggtt accattttct gcaaagggtc cactggagcc
      481 gtaaccacga tcgaatacga aggtggagtt ctggaggacc taaagaaagc catcgaaaga
      541 atagctccct ccaacatccc ctacgctcac gaccaaagat ggggagatgg aaacggcttc
      601 tcccacgtcc gggccgccat tctcggcccc tccctcacgg tgcccatctt tcagagctcc
      661 ctggccctgg ggacctggca gcaaatcgtc ttcatcgatt ttgacaaccg gagcaggaga
      721 aggaatatta tggttcaggt aatgggagaa tgaaagacag acgcatggag ggggagacga
      781 gaagacacgg ggataaatcc gtgaaacccc acctggattt ccgatccttt attccagaaa
      841 ttctgaatcc tgaatttgaa ttttcccagg taaaatatta aggctgacgc atgatatcat
      901 tggcaagttt ctctcaatac cttattaccg taacgcgtcc gtctctttaa tgccttagct
      961 gcaaaactta atgtttgggc ggtgatcagt tacggtagga ttggtggttt tttcatcttt
     1021 cacctcttgc gaaagcgagg aagaaacatg ggcacttcct gcatgtattt tgcgtagtca
     1081 tccccaaaac gggcgagaag cagcttttct tcgtatttcg aaatatagtg cagaaaaacg
     1141 attgtaaccc cccacacccc agcagcggcc aaggaaaggc taagcatcac gagagcaaga
     1201 taaaaaagaa tttcggacag gtacacgggg tgacggacga cgccgaagac gcctttccga
     1261 atcacggcgg gcttctctct cctctcgccg aatacgattg ccagacccgt tctcgccaag
     1321 taggccgaaa gaataaatag gaggactcct aaggggattc ttgcccctag gtgaatatat
     1381 ctgttgagga aggtcgtgta ctgaaaaaag aaagtgtcgg cgatccagac tcctataaac
     1441 agacaggcca ggatgatctg accggcgtcg ccaaaggcat gttcgccggt gagatcgtcc
     1501 cggtcatttt ttgctttaat cgatgctttc tcggagacca cctcgattcc cctccagcca
     1561 agcgctctgt gagcagcccc tctgcgaaag ccccgtcggc gtttactata gcataaagaa
     1621 atgacaacgg ttctatttta gatttatgac cgatcagtga aggtccttaa ggtatggcaa
     1681 aatccgggta tggaaaaatc cgggctttga aaaatcaaga accgagatga cctcgactca
     1741 ggcaggtgtt cgggcgacct gccagattat ctgaaggccc tcgcttccgg gaaggaagtc
     1801 cagaaaaaaa tcttctccga cacggtagta aaaccctata cctacaaatc aatgaggctc
     1861 ccttatggct ctatgcttcc gcagaaggtg gagaacctgc cggtggcggg aaggagccta
     1921 tcttaagcct acccttggcg aaaagtggag gtagagactc gctgggttgt caggataaat
     1981 gggatatggt agtatccttt tcatggaata cataagtacc cgggggtctg cgccggccat
     2041 tccttctaag aagacgatca taaaaggtat tgcagaagac aatggtttat acattccctc
     2101 cacctttcct tctttcggtc cgtccccctt tgtaggcgtt caaaggaaat cctactctga
     2161 acgagcggtc aaaatcctca gccccttcct ctcggattac acagacgaag agctgaatac
     2221 cgcctgcggg gacgcctatg caaagagttt cgaatccccc caaatcgctc cgttgagaat
     2281 ccttggagac ggaactgcgg ttttggaact ctggcacgga cccacccagg ccttcaagga
     2341 catggctctc cagctcatgc cccgtcttct ttccttcgcc ctaaaagggg aaaaggaaaa
     2401 atacgaggtg gtcatcctgg tggccacttc cggggacacg gggaaggccg cgctggaggg
     2461 ctttgccgat gccgaggggg tacgcatctt cgtcttctac cctcacggag gtgtgagtga
     2521 aatccagcgc ctgcagatgg tcacccagca gggaaagaat gtgggggcct gcgcagtcaa
     2581 ggggaatttt gacgatgccc agaccggggt gaagcaaatc ttttctaatt cggctctcaa
     2641 ccggaagctg gccgactttc attttcagct ttcttccgcc aattccatca actggggccg
     2701 cctggccccg caggtcgcct actacttcac cgggtacgat gatatggtgg cggccggcaa
     2761 ggtccggcag ggccagccta ttctgatttg tgttcccacc ggtaactttg gaaatatcct
     2821 ggcggcctac tacgctatcc gctgcggcct tccggtgtcc cggttgatct gcgcttccaa
     2881 ccggaacaag gtactcacgg aattcatccg caccggggtg tatgaccggc ggagggaatt
     2941 tttcaagacc gaatcgccct ccatggacat ccttatctcc tccaacctgg agcgtctgct
     3001 ctacgagttg agcgggcgta aagcgaatgt ggtggttcaa tggatgaagg acctcagcat
     3061 ccagggccgt tataaaatcg atcagcgggg gttggaggag ctccagaagt tcttctatgg
     3121 aggctttgct gacctgaggc aaaccctcgc tgccatccga agggccttcg agaagcaggg
     3181 atatttgatg gatacccaca cggccgtggg atacagcgtt ctgcaggagt accgggaaga
     3241 gacgggggac ggcactccgg ccctgttggc ctccacggcc agccccttca agttcaacca
     3301 gtcggtgctg gaggctctgg gccgggacac ttcggggaag gacgagttca ccctcctgga
     3361 ggagctttcg gccatgagcg gccagccggt cccggtcaag ctgaccgagc tgaagtctct
     3421 gcccgagctt cacggcgggg tgtgcgagag agaagggatg gcccaggccc tcttccagtt
     3481 tcttaaggtc ccctaacaag ccccggcgtg attgtagagg cggttccctg tagcaagacg
     3541 ggcaaccacg gggggttgcc cctacaattt gcggcggata gcgatggaac gatgaacgct
     3601 ca
//
LOCUS       DASPRR010000068         4331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2275177, whole genome shotgun sequence.
ACCESSION   DASPRR010000068 DASPRR010000000
VERSION     DASPRR010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275177"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VEL68_02410"
     CDS             <1..569
                     /locus_tag="VEL68_02410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXZ34854.1"
                     /translation="THLDAPWHYAPVSQGKKAKTIDEIPLDWCYGNGVCLDFRRKKDG
                     EEITIGDLEKALKKICYKIKPKDIVLIMTGADKFWGKPEYLIKGAGMGRESTLWLLNR
                     GVKVVGIDAWSWDRPLPFLKKEFQEKGDATILWAAHFAGIKKEYLHMEKMANLQKLPP
                     HGFTLVCFPVKVKAASAGWVRPVAIVKE"
     gene            complement(644..1498)
                     /locus_tag="VEL68_02415"
     CDS             complement(644..1498)
                     /locus_tag="VEL68_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXZ34855.1"
                     /translation="MEKVIISAALAGAATRKEQNPAVPYTPQEFAEESFKCWQAGAAI
                     VHIHARDPKTGNPTADIPQIRETIDAIRNRCPDLIINMSSAIGPWVTPEQRIAPIVEI
                     KPEMASLNTNSMNFALADHKSGKIFGEIIFQNTFKMLVDFGTAMKENRVKPECEVYDF
                     GGIYNVLLVRKQGIFIEPMHFQLVFGVAGGVPFSPMNMIHMQSLLPEGATWSTCGVGP
                     NQFPAGIMASIMGGHIRVGLEDNTRVLRGKLAEGSWEQVVVAKRIAELAEREIATPTE
                     ARKMLNLK"
     gene            complement(1659..2678)
                     /locus_tag="VEL68_02420"
     CDS             complement(1659..2678)
                     /locus_tag="VEL68_02420"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ34856.1"
                     /translation="MKSAVLTGKRTIEIQDVPTPSPQKDEALVRVHFCGICGSDVHIF
                     QHGLPQPNILGHEFSGVIAELGPEVENWRVGEAVSCVPGIKCGVCYWCQNGQSQICEW
                     ALQKSYGTGVVPGAMAEYVVVKASSLRRLPQGVGAAEACLAEPLSVCLHGVRLSRVRP
                     GDRAVVLGCGPIGLITTLILVRSGVRSVHCTDLAESKRRRALKLGAATVHDPKGLSPF
                     IFHQITDNLGPDIVYECVGVPQTLVEGINYVKKGGQVLVLGVGMDPTTLLPMVWNFKE
                     VEIKGSYGMTGEEYDLALEWLARRWVPVEQIITREVPLDEVKWAFNLLEGPNEEVKVL
                     VRVGG"
     gene            complement(2741..>4331)
                     /locus_tag="VEL68_02425"
     CDS             complement(2741..>4331)
                     /locus_tag="VEL68_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXZ34857.1"
                     /translation="YIFGLSGGHINTIFDACIDFGIKIIDTRHEQAAVNMAEGWARFT
                     GKPGVAVVTAGPGAVNAFPGVAVAMQSGSPVVIISGRSSIERRDIGAMQDMDQIEVMH
                     PVAKWSRSVYQTNRICEYVTSAFRQAQSGRPGPVFLEIPIDIVDGQSHIEQVVWCKGY
                     RTEHRPYGDPLAVERAADLLSKAQRPVIIAGGGAWWSGAGEELRAFVEATHIPFYSRS
                     MARGIIPDDHPLSGGMFPAGLTQADVALILGTKLDWTIAYGRPPIFHPEVKVIQVDIE
                     QEEIGKNRPVDVGIPGDVQAVLAQLRQALQGKYKAPESWAATVKAMMGGVRTQFAGDI
                     RKTGSPVHPARLAQEVREFLPRDAALVIDGGDIAVFANVLLDAYSPGSLVWVGGFGHL
                     GVGVPYANAAKLAHPERQVALLTGDGSFGLSLMELDTAVRHRIPIVCVVANDGGWGQI
                     RRGQIAKYGRDRVVGSQLGTRPYHKIVEAMGGYGELVERTEDIKGAMERAFKSGLPAC
                     INVITDPEPKFPGMDFPWQIT"
BASE COUNT          894 a         1326 c         1177 g          934 t
ORIGIN      
        1 gaacccacct ggatgccccc tggcattacg cccccgtttc ccaggggaag aaggccaaga
       61 ccatcgacga gatccccctg gattggtgct atggcaacgg cgtatgcctg gatttccgga
      121 ggaaaaagga cggcgaagag attaccatcg gagacctgga gaaggccctg aaaaaaattt
      181 gctataaaat caaacccaag gacatcgtgc tgattatgac gggtgcggac aagttctggg
      241 gaaagcccga atacctgatc aaaggggcgg gaatgggccg ggagtccact ctatggcttt
      301 tgaatcgggg ggtcaaagtg gtaggcatcg acgcctggag ctgggaccgt ccgctccctt
      361 tcctcaagaa agaattccag gagaaggggg atgccaccat cttgtgggcc gcccattttg
      421 cagggatcaa aaaggagtac cttcatatgg agaaaatggc caatcttcag aagctgcctc
      481 cccacggttt taccctggtt tgcttcccgg ttaaagtcaa agccgccagt gcgggatggg
      541 tgagacccgt ggccattgtg aaagaataaa gaatcaatta ccaatgatca attagcaatg
      601 gtcaatgccc aatgattatt gattattgcc cattgattat tgctcatttt aagttcagca
      661 tcttcctcgc ctctgtcggg gtggcgattt ctctctcggc cagctcggcg atccgcttgg
      721 ccactaccac ctgttcccag cttccttccg ccagcttgcc cctgagtacc cgggtgttgt
      781 cttccaatcc cacccggatg tggcccccca tgatggaggc catgatcccg gcgggaaact
      841 ggttcggccc cacgccgcac gtggaccacg tcgccccttc aggcagcagg ctctgcatgt
      901 ggatcatgtt catggggcta aagggaactc ccccggccac gccaaagacc agttgaaagt
      961 gcatgggctc tatgaaaatt ccttgttttc tcaccaaaag gacgttgtag atacccccga
     1021 agtcatacac ctcgcattcc ggcttcaccc tgttctcctt catggccgtg ccgaaatcga
     1081 cgagcatctt gaaggtgttc tggaagatga tctccccgaa aatcttcccg ctcttgtggt
     1141 cggccagggc gaagttcatg gagttggtat tcaaagaggc catctctggc tttatttcta
     1201 cgatcggggc aatgcgctgc tctggtgtta cccacggtcc gatggccgag ctcatgttaa
     1261 tgattaaatc cgggcaacga ttccggattg catcgatggt ctcccgaatc tgggggatat
     1321 ccgctgtggg gttccccgtt ttggggtccc gggcatggat gtggacaatg gccgcgccgg
     1381 cctgccagca tttgaaagac tcctccgcaa actcctgggg cgtataggga accgccggat
     1441 tttgctcctt ccgagtggcg gcccctgcca aggctgcaga gatgatcacc ttttccattt
     1501 ttgccctcct ttgagcttgc tattcggggt gagccattac gagattgatt ctagatggat
     1561 ccaaaaataa agaagaggga agatcttatc agcaaaacct ttgagtatga aggttcaaat
     1621 ccagcctgga ccgtccaacg aaggatcccc ggcttgaatc agcccccgac gcggaccagc
     1681 actttcactt cttcgttggg cccttcgagg aggttgaagg cccacttgac ttcatccagg
     1741 gggacctcgc gagtgatgat ctgttccact ggcacccatc tgcgagccag ccattccagg
     1801 gccaaatcgt actcctcgcc ggtcatccca taggaccctt tgatctccac ttctttgaag
     1861 ttccagacca tggggagaag cgtggtcggg tccatcccca ccccgaggac caggacctgg
     1921 ccgcctttct tcacataatt gattccttca accagcgtct gcgggacccc tacgcattca
     1981 tagacgatat ccgggcccag gttatccgtg atctggtgaa agatgaaagg agacagacct
     2041 ttgggatcat ggaccgtcgc ggcacccagt ttcaacgctc gccggcgttt ggattccgcc
     2101 aggtccgtgc agtgaacgga ccgcactccg gaccgaacta aaatgagggt cgtgatcagg
     2161 ccgatcgggc cgcagcccag gaccacggcg cggtctcccg ggcggactcg gctgagacgt
     2221 accccatgga ggcaaacgga aaggggctca gccagacagg cctccgctgc cccgacccct
     2281 tggggaaggc gacgcaggct ggaggccttt accacaacgt attccgccat cgcccccggg
     2341 accactcctg tgccgtagga cttctgcagg gcccattcgc agatctgcga ctgtccgttt
     2401 tgacaccagt aacacacccc gcacttgatt cctgggacgc agctgaccgc ctccccgacc
     2461 ctccaatttt caacttcagg gcctaattcg gcgatgaccc cggaaaactc atgccccaag
     2521 atgttcggct ggggaaggcc gtgctgaaag atatggacgt ccgatccgca gattccgcag
     2581 aaatggaccc ggaccagcgc ctcgtccttt tgtggactgg gggttgggac gtcctggatt
     2641 tcaatggtgc gctttccggt gagaaccgca gacttcatct tcccctcctg acgaaccaag
     2701 ggagttattc aaaacctgaa acttgaaacc cgaaacttct ttaggttatc tgccacggga
     2761 aatccatgcc cgggaatttg ggctccgggt cggtgattac attgatgcag gccggcagtc
     2821 cgctcttgaa ggcccgttcc atggccccct tgatatcttc cgtacgttcc accaattccc
     2881 cgtaccctcc catggcctcg actattttgt ggtaaggcct cgtcccgagc tgggaaccga
     2941 caactctgtc cctcccgtat ttcgcgatct gtcccctccg gatctggccc cagcctccgt
     3001 cattagccac aacacaaaca atgggaatcc ggtgccgaac cgccgtgtcc agttccatga
     3061 gagaaagacc gaaggaaccg tcgccggtga gtaaggccac ctgcctttcc ggatgagcca
     3121 attttgccgc gttggcatag ggcactccaa ctcccaggtg gccgaatccg cccacccaca
     3181 ccagggagcc tgggctgtag gcgtccagga gaacgttggc aaagaccgcg atgtcgcctc
     3241 cgtcgataac cagcgcggca tcccggggca gaaactcccg aacttcctgg gccaaacggg
     3301 cgggatggac cgggcttccg gtcttgcgaa tatccccggc aaattgggtc cggacccccc
     3361 ccatcatggc cttaaccgtg gccgcccagc tttcgggggc cttgtacttc ccttgaaggg
     3421 cctggcgcag ttgggcaagg accgcctgaa cgtctccggg aatccccaca tccactggtc
     3481 ggtttttccc gatctcctcc tgttcaatgt ccacctggat aactttcacc tccggatgaa
     3541 aaatgggagg ccgtccgtag gcgatggtcc aatctaattt cgtccccaat atgagggcca
     3601 catcggcctg ggtgaggccc gcagggaaca tgcctccgct gagcggatga tcatccggga
     3661 tgattccccg agccatgctc cggctataga aagggatgtg cgtcgcttcc acaaaagcgc
     3721 ggagctcttc cccggctccc gaccaccagg ccccgccccc ggcgataatc accggccgct
     3781 gggccttgct caagaggtcg gctgccctct cgacggccag agggtctcca tagggccggt
     3841 gctcggtccg gtaccccttg caccagacga cttgctcgat gtgcgactgc ccatcgacga
     3901 tgtcgatggg gatttccagg aagaccggcc cgggtctccc actctgggct tgacggaagg
     3961 cgctggtcac gtactcgcag attcggttgg tttgatagac gctgcgcgac catttggcca
     4021 ccgggtgcat gacctcgatc tggtccatgt cctgcatggc cccgatgtcc cttctttcga
     4081 tggagctcct ccccgaaata atgacgactg gtgaaccgct ctgcatggcc accgcgaccc
     4141 cggggaatgc gtttaccgct cccgggccgg cggtaaccac cgccaccccc ggctttccgg
     4201 taaatctggc ccatccttcg gccatgttca ccgcggcctg ttcatgcctc gtgtcgatga
     4261 tcttaatccc aaagtcaatg cacgcatcga agatagtgtt gatgtgcccc ccggaaagtc
     4321 cgaagatgta c
//
LOCUS       DASPRR010000069         8382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_502225, whole genome shotgun sequence.
ACCESSION   DASPRR010000069 DASPRR010000000
VERSION     DASPRR010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502225"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..360
                     /locus_tag="VEL68_02430"
     CDS             190..360
                     /locus_tag="VEL68_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34858.1"
                     /translation="MIFLAIGAAICVTLGSLLRFIIRNPVRRLAAYPIYLGYILFTAS
                     IAIYIVLGFLSP"
     gene            401..1363
                     /locus_tag="VEL68_02435"
     CDS             401..1363
                     /locus_tag="VEL68_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ34859.1"
                     /translation="MGKQSAKIFTLLFALSFCLSLGWAAEKYPDRPIDFICTWGVGGG
                     ADQMARTIGKLAEKFLGVALPVSNIPGSSGNSGMANVLAAKADGYTVATYIADTLGTI
                     PAGTARHKITDFEWIVRTQVAQSYLFVKMDSPFKTIQDLLNYAKENPGKVKVAATGFG
                     TVDDITVRYLASKGYKMTTIPIPQPGERYASTLGGHSEVLYEQAGDIKQYLEANQLRP
                     LIVFSQKRFPAFPDLPCSLELGYEITLPQFRSIVAKKGVPGDRVKILAEAFQKAMETP
                     EWKKFAQDQYLDPESYLGPDKFPAWIASEVETLRNFMKTFGMAK"
     gene            1408..1965
                     /locus_tag="VEL68_02440"
     CDS             1408..1965
                     /locus_tag="VEL68_02440"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ34860.1"
                     /translation="MSKIDAGQAQHARLEPILKSLIGPLLIFLLAVYFYFLAGKIDEP
                     PSPEQLGAAFWPKMILIFLMISCGIKAGEILMARRKGGAEGTPAGPRPEVDIPKLAAM
                     IAMVIAAVYFMDIIGFPLCNFIFLILFMRIAGLRKKIPLFLTSVLGTIFLLYLFVKIV
                     YLPLPKGQWVFDDMTIFLYRLLHII"
     gene            2062..3561
                     /locus_tag="VEL68_02445"
     CDS             2062..3561
                     /locus_tag="VEL68_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ34861.1"
                     /translation="MFDQLALLGTGLVNTFDPLNFSMILVGLVIGVLAGALPGITMLN
                     AIVLVLPFTYLMGIVPSLLLMTSVYCGGVFGGSITGILFNIPGDPMNVPTTWEGYQLN
                     KKGFVSKALGMAIMCSAIGGFLSALIMTFVSPPFAKVALSFSSCEYFSVVFLGLVSVV
                     VIGTRSIGSAFISLFIGMFLGTVGMDDIFGAVRFTFGSRIMETGINFTTVLIGLFAIG
                     EVLDQLSIKKKGEGEIIRTRPKTTLPRLGEIWGLRNTVLRGLGIGTVIGAIPGTGATV
                     ASFVSYGVEKQVSKTPEKFGTGVWEGLVASETSINASTGGAMIPLLTLGIPGSGATAV
                     MMGAFLLHGIQPGPLLFNKSPEAVYTIFVGMLVCNLIMILAGLITAKFFSELMRVPEN
                     ILCAFIITFCLLGAFALRNDMADVWFMAIFGIVGYFMRRYELPIPPMILGIILGPLAE
                     KYFLTTMIGAGNDFTIFFTRPVSGCLMVISVGLLLMPFLRGLGKKGKKT"
     gene            3570..3992
                     /gene="glmS"
                     /locus_tag="VEL68_02450"
     CDS             3570..3992
                     /gene="glmS"
                     /locus_tag="VEL68_02450"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HXZ34862.1"
                     /translation="MSDRKETKTVVLGVLGVDAHVVGNKIMAHALEAGGFKVVNIGTF
                     SSQQDFIRAAIETAAHAILVGSLCGHGEMECRGFRENCIEAGIGEVYLVVGGNLVVGK
                     QKWEEVEKRFLQMGFNRAYPPGVSPRKVIEDLKKDLGN"
     gene            4075..5460
                     /gene="glmL"
                     /locus_tag="VEL68_02455"
     CDS             4075..5460
                     /gene="glmL"
                     /locus_tag="VEL68_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HXZ34863.1"
                     /translation="MSLALLIDFGSTFTKILAVDLTAEEILAYAQSCTTVDTDIMVGL
                     RRALELLPPLLKNSPYECKLACSSAAGGLGMITIGLIPELSAEAARRAALGAGAKVVK
                     VYSFRLSTRELEELEGLKPDILLLAGGTDGGNREVILDNARLLAGSRIECPILIAGNK
                     AAADEVQSILRAGGKITLLANNVMPEVNVLNVEPARDMIRKVFIERIIAAKGLKKAEE
                     FVEGILMPTPTAVLRAAQLLSQGTGSEKGMGDLVVVDIGGATTDVHSIGMGSPVQAGV
                     VRKGLPEPLAKRTVEGDLGLRYNAATILKLCGEGRILQHTSLFQKDLSSRLEVLSQCV
                     EVLPQTAEEQDLDFALAACAAQAAMERHAGSIEALWGPQGQYYLQYGKDLTGVEHLIG
                     TGGIFIHHPQAGEILRMTLFSPQEPFSLRPKNPNIYSDARYCLYAVGLLADRYPETAS
                     RIARKYLKKWD"
     gene            5612..7054
                     /locus_tag="VEL68_02460"
     CDS             5612..7054
                     /locus_tag="VEL68_02460"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HXZ34864.1"
                     /translation="MKLSNQRWSDEEFFAEREKVLTLWPTGREIDLEETVDYLKSLPP
                     AKLYARVIVEAEKEGRTLVQPRGGVALIDNHIALLRCLQDKGGADLLPTTTDTYTRNL
                     KFQDAQRGMEESRRLGRSMLNGCPIVNHGFRAIRRISESVQRPIIVLSGTAFPQLTAE
                     MGFAGGMTAFLGAAISYTASYTKDLPFEQGIRNYQYLDRLTSYYEQRGIRLHREQPGF
                     LTGTLIPPGIGIAVAVLEMLLAAGQGVRHYSMGLCQSLNISQDVAGLWALKEVGREYL
                     RRFNHEDLFFSVATHQWMQAFPPDEPRAFAVIVLGGIIAALAGATQVITKSTHEAEGI
                     PTQEANAEGVKATRMAIQLLRACRLPESPEVSQEKEMIIKEARSILDKALEMGDGDPA
                     LASLRSLQAGVLDIPWAPNQFVAGRVIPVRDASGAIRYLEHANLPFEREILEYNLEKV
                     REREHKAGKKINYEEAIFDITEVSRMLENE"
     gene            7163..7795
                     /locus_tag="VEL68_02465"
     CDS             7163..7795
                     /locus_tag="VEL68_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HXZ34865.1"
                     /translation="MKFASPEIIARVVESLKKNEMEAVYFSTVAEAKNEVLRRIPLGA
                     KVGIGGSITLREMGIPEVLEKKGHEVYDHWREGLSKEKRQEVGKKHQRADVFLTSTNA
                     LTLDGKLINVDATGNRVSSMIFGPEKVVVISGVNKIVRNLSEGLARIKKVAAPRNCQR
                     RKDPTPCAQDLICRNCNTPARLCRVTTIIERRPWGIKDFSVILVGEELGY"
     gene            7842..>8382
                     /locus_tag="VEL68_02470"
     CDS             7842..>8382
                     /locus_tag="VEL68_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34866.1"
                     /translation="MKIERIDLCRLKIPLKQPYKIATAEMKTFDCTIVCLHSQRREGL
                     GEAMADIQGYFWETADQVWRFAKEQGPKILGLQVEKARDSISRHAKEMPCSVTPFLTA
                     LEMLNPKSLLAPPSQLISVPMLGILQATDPEGIRAEIEDFISRGYDTIKIKVGFDVDR
                     DLERVKLAQEMIQGRAHLRA"
BASE COUNT         1881 a         2261 c         2361 g         1879 t
ORIGIN      
        1 tgacgaatac ctggactata agaagcaagc tctggaaatc ctccacgaag aggccatgag
       61 ggcgttcgcc accggcagcc ggttagcggt ggccgatttt ctggaggcat acgccgagat
      121 ggggaagaaa cccgaggaaa aggggaaaga acctcattga tcttgtccct ttttttatcg
      181 cgggaggcgg tgatcttcct ggcaattggc gctgcgatct gtgtcacctt gggaagcctt
      241 ctccggttta tcatccggaa tccggtcagg cgacttgccg cttacccgat ctacctggga
      301 tacattcttt ttacagccag catagcgatc tacattgttt tgggattctt atcaccctga
      361 gtttcgatgt aaaatttttc cgcccggaaa ggagcaaacc atgggaaaac aatccgccaa
      421 aatcttcact cttttattcg ccctttcatt ttgcctttcc ttgggttggg ccgccgaaaa
      481 gtatcccgac cggcccatcg acttcatttg tacctggggg gtgggcgggg gggccgacca
      541 gatggcccgg acgatcggga agttggccga gaagttcctg ggcgtggccc tgcctgtctc
      601 gaacatcccc ggttcttcgg gaaactccgg catggccaac gttctggccg ccaaggctga
      661 cggatatacg gttgccactt acatcgccga cacgctgggc acgatccctg ccgggaccgc
      721 ccggcacaag atcaccgact ttgaatggat tgtccgaacg caggtggccc aatcttatct
      781 gtttgtaaaa atggacagtc cctttaaaac catccaggac cttctgaatt atgccaaaga
      841 aaacccggga aaagtgaaag tggcggccac tggttttggg accgtcgacg acattacggt
      901 ccgttacctg gcctccaagg ggtacaagat gaccaccatt cccatccccc agccaggaga
      961 acgctacgct tcaaccctgg gggggcattc ggaagtgctc tatgagcagg ccggggacat
     1021 caagcaatac ctggaggcca accagcttcg ccccttgatc gtcttctctc aaaagcgctt
     1081 tccagccttc cccgacctcc cctgctcctt ggaattgggc tatgagatta cccttcccca
     1141 gttccggtcc atcgtggcca agaagggggt cccgggtgac cgggtgaaaa tcctggcgga
     1201 ggccttccag aaggccatgg agactcccga atggaagaaa ttcgcccagg accaatacct
     1261 cgacccagag agctacctgg gtccggacaa gttcccggcc tggatcgctt ccgaagtgga
     1321 gaccctgcgg aatttcatga agaccttcgg tatggctaag tagggcgagg aatccgaccc
     1381 tactgagtta ttgaaaagag gatagccatg tccaaaatcg atgccgggca agcacagcat
     1441 gcgaggttgg agcctatctt gaaatccctg atcggacctc tcttgatctt tcttctggcg
     1501 gtttattttt actttctcgc cgggaagatt gatgaacccc ccagtccgga gcagttgggc
     1561 gcagcctttt ggcccaagat gattctcatt tttcttatga tcagctgcgg gatcaaagcc
     1621 ggtgaaattc tcatggcccg tcggaaaggg ggggcggaag ggaccccggc aggacctcgt
     1681 cccgaagtgg atattcccaa actggcggcg atgatcgcga tggtcatcgc cgcggtttat
     1741 ttcatggata tcatcggatt tcctctgtgc aatttcattt tcctcatcct tttcatgcgc
     1801 atcgccgggc tgcgcaaaaa aatacctttg ttcctgacct ccgtcctggg gaccatcttt
     1861 ctcctttacc ttttcgtgaa gatcgtttat cttcccctgc ccaaggggca gtgggttttc
     1921 gacgacatga ccatttttct ctaccggctt ttgcatatca tttgagatcg ttagggacga
     1981 ttcatgaatc gcccccacgg aaaagtgcgg aattcaaccg cacggcggtt cgtatagcga
     2041 atctggcaag gagaccgctc catgttcgat caattggcac ttctgggcac cgggttggta
     2101 aacacttttg atcctttgaa cttctccatg attctcgtgg ggttggtcat cggagtcctg
     2161 gccggggctc tcccgggcat caccatgctc aacgcgattg tcctggttct gcccttcact
     2221 tatttgatgg gaatcgttcc ttcccttctc ctcatgacct ccgtatattg cggaggagtc
     2281 ttcggggggt ccattaccgg aattctcttc aacatccccg gcgaccccat gaatgtcccc
     2341 acgacctggg aaggctacca gctgaataag aaaggcttcg tgtccaaagc tttaggaatg
     2401 gccatcatgt gctcggccat cggcggattc ctgagcgcct tgatcatgac ctttgtctct
     2461 cccccttttg ccaaggtggc tctctctttt tcctcctgcg agtacttttc ggtggtcttc
     2521 cttggcttgg tgagcgtggt ggtcatcggc acccggtcga tcggttcggc tttcatatcc
     2581 ctcttcatcg gtatgttttt gggcaccgtg ggcatggacg atatcttcgg tgcggtgcgc
     2641 ttcactttcg gatctcgaat tatggagact gggatcaact tcaccaccgt cctcatcggt
     2701 ctttttgcca tcggggaagt gctcgatcag ctttccataa agaaaaaagg ggaaggagaa
     2761 atcattcgca cccgccccaa aacgaccctc ccccgccttg gagaaatctg ggggctacgg
     2821 aatactgttt taagagggtt gggcattggc acggtcatcg gggcgatccc cggcactggg
     2881 gcaacggtcg cttcctttgt cagctacggc gtggaaaaac aggtctccaa gaccccggag
     2941 aaattcggca ccggcgtctg ggaagggttg gttgcctcgg aaacctccat caatgcttcc
     3001 accggaggcg ccatgatccc cctcctcacc ctcggcatcc ccggcagcgg ggccaccgcg
     3061 gtgatgatgg gggcctttct ccttcatggc atccagcccg gaccgcttct cttcaacaag
     3121 tcgcccgagg cggtttacac catttttgtg ggaatgttgg tctgcaacct aatcatgatc
     3181 ctggccgggt tgatcaccgc caaattcttt tccgaactga tgcgggttcc ggaaaatatc
     3241 ctctgcgctt ttatcatcac cttctgcctc ctcggggctt ttgccctgcg caatgatatg
     3301 gccgatgtgt ggttcatggc catttttgga atcgtgggat acttcatgcg ccggtatgaa
     3361 ctccccattc cccccatgat cctgggcatc attctcgggc ccctagcgga gaagtacttt
     3421 ctgacgacca tgatcggcgc gggcaacgat ttcaccatct tcttcacccg gccggtaagt
     3481 gggtgtctaa tggtcatctc cgtggggctg ctcctcatgc ctttcctgag aggcttggga
     3541 aagaaaggca agaaaaccta ggctcaatca tgagtgatcg gaaggaaacc aagaccgtcg
     3601 tcctgggagt cctgggagtg gacgcccatg tggtgggaaa caagatcatg gcccatgccc
     3661 tggaggccgg cgggttcaag gtcgtcaata tcggaacttt ctcttcccag caggatttta
     3721 tccgtgcggc catcgaaacc gcggctcacg ctatcctggt gggttccctc tgcggccatg
     3781 gggaaatgga gtgccgcggg ttccgggaaa attgcatcga ggcgggaatc ggtgaggtct
     3841 acctggtcgt cggcggaaac ctggtggtgg gtaagcagaa atgggaagaa gtggagaagc
     3901 gctttcttca gatgggtttc aaccgcgcct acccccccgg agtttccccc cggaaggtca
     3961 tcgaggactt gaagaaagac ctggggaatt gaaaagacga gtagagcatc gagcatagtg
     4021 cagaggaatt tttcttttcg ctatgctcca cgcgatatgc gttatgcatt ctgtatgtcc
     4081 ttagcccttt tgatagattt cgggagcact ttcaccaaaa tcctggcggt cgatttgact
     4141 gccgaagaaa ttctcgctta cgcccagagc tgcacaacgg tggacacgga tatcatggtg
     4201 gggctgcggc gggccctgga gctcctcccc cctcttctga agaactctcc ttatgaatgc
     4261 aagctagctt gcagcagtgc ggcgggtggc ttgggaatga tcaccattgg cctgatcccg
     4321 gagctttcgg cggaggccgc ccgtcgggcg gccctgggag ccggggccaa agtcgtcaaa
     4381 gtttattctt ttcgtctttc aacccgggaa ctggaggaac tggagggttt gaagccggac
     4441 atccttctcc ttgccggggg aacggatgga ggaaaccgag aggtcatcct agacaacgcc
     4501 cgtctgcttg cgggcagcag gatcgagtgc cccattctga ttgccgggaa caaagccgcg
     4561 gcggacgagg tccaatccat cctgcgcgcc ggggggaaaa tcacccttct ggccaacaac
     4621 gtcatgccgg aagtgaatgt gctgaatgtg gaaccggccc gggacatgat ccgcaaagtg
     4681 ttcatcgagc ggatcatcgc cgccaagggt ttgaagaagg cggaggaatt tgtcgaaggc
     4741 atcctcatgc ccacgcccac cgccgttttg cgtgccgctc agcttctttc ccaggggacg
     4801 ggttccgaga aagggatggg agacctggtc gtggtggata tcgggggggc tacgaccgac
     4861 gtgcattcca tcggtatggg gagtccggtc caggccggag tggtgcgtaa aggcctgccg
     4921 gagcctttgg ccaaacggac ggtcgaaggc gacctgggcc tccgttacaa tgccgccacg
     4981 attcttaagc tgtgcgggga gggacgaatt ctccagcata ccagcctctt ccaaaaagac
     5041 ctttcctcga ggcttgaggt gctttctcag tgcgtggaag tccttcccca aacggcggaa
     5101 gaacaagatt tggattttgc cctggccgcc tgtgcggccc aggcggcaat ggaaaggcat
     5161 gccgggtcaa tcgaggctct ctggggtccc caaggacaat actaccttca gtatggaaaa
     5221 gacttgaccg gcgtggaaca cctcatcggg acgggaggaa ttttcatcca ccatcctcaa
     5281 gccggcgaga tcctgaggat gacacttttt tctccccaag agcccttttc cctgcgcccc
     5341 aagaatccaa atatttacag cgatgcccgg tattgccttt atgcggtggg tctgcttgcg
     5401 gaccgctatc cggaaacggc ctcccgaatt gccagaaaat acctaaaaaa atgggattaa
     5461 aatttttacc acagagcgca cagaggccgc agagaaagaa ttagatttaa aaagaagaag
     5521 agaagaacga aagagggtat ccccataaaa aaatttttcg gatttaacca attccttgtg
     5581 ttctctgggt tctctgcggt taagggaaaa aatgaaactg agtaatcaac gatggtccga
     5641 tgaagaattc tttgccgaac gggagaaggt ccttaccctc tggccgaccg ggagagagat
     5701 cgatctggag gaaacggtag attatttgaa aagcctcccc ccggcgaagc tgtacgccag
     5761 ggtgatcgtc gaggcagaaa aagaaggccg caccctagtc caaccccggg gaggggttgc
     5821 tttgattgac aaccatatcg ccctactccg atgtttgcag gataagggag gggccgacct
     5881 ccttcccacg acgacggata cctacacgcg caacttgaaa tttcaggacg cccagcgggg
     5941 catggaagaa agccggagac tgggccgctc catgctcaac ggctgtccaa tcgtcaacca
     6001 cggttttcga gccattcgcc gaatatcaga atcggttcaa cgtccgatca tcgtcctctc
     6061 tggaaccgcc tttccccagc tcaccgcgga aatggggttt gccgggggca tgaccgcctt
     6121 tcttggcgcg gccatttcct acactgcttc ctacaccaaa gacctcccct tcgaacaggg
     6181 aatccgcaac taccagtatc ttgaccgcct gacttcttat tatgagcaaa gggggattcg
     6241 ccttcaccgg gaacagcctg gattcctcac cggcactctt atccccccag ggatcggaat
     6301 cgcggtggct gtgctggaga tgctcctggc ggcgggccag ggagtcaggc actacagcat
     6361 gggtctttgc cagagcctca atatttccca ggacgtggct ggcctatggg ctttgaagga
     6421 ggtcggaagg gaatacttgc ggcgtttcaa ccatgaagac cttttcttct ccgtagctac
     6481 ccaccagtgg atgcaggcgt tcccgccgga cgagccccgg gccttcgcgg tcattgtgct
     6541 gggcggaatc atcgctgctc tggctggagc tacccaggtc atcaccaagt ccacccacga
     6601 ggctgaaggg attcctaccc aggaagcaaa tgccgagggc gtcaaagcca cgaggatggc
     6661 cattcaactc ctacgagcct gccggctgcc ggaaagcccg gaggtttccc aagagaagga
     6721 aatgatcata aaggaggccc gctccatcct ggataaagcc ctggaaatgg gagacggtga
     6781 ccccgccttg gcgtccctcc ggagtctaca ggccggggtc ttggacattc cttgggcccc
     6841 caatcagttt gtggcaggca gggtcattcc ggtgcgggat gcttcgggag cgatccggta
     6901 cctggaacat gccaaccttc ctttcgaacg ggagatattg gaatataacc tggagaaggt
     6961 ccgcgaaagg gagcataagg cgggcaaaaa gatcaattat gaagaggcga tcttcgatat
     7021 tacggaagta agcaggatgc tggagaacga gtgagacgtg aggagcaaag tttgaggaca
     7081 aagaaaaaag agcgggggtc ggtccatacg gcagaaaagg acaaacaggc aatattgaat
     7141 tgaaatgagg gaaaattatt ctatgaaatt cgcatccccg gaaatcatcg ccagagtcgt
     7201 cgagagtctg aagaagaacg aaatggaggc tgtgtatttc tccaccgtgg cggaagctaa
     7261 gaacgaggtc ttgcgtagga tccctctagg agccaaggtg ggaatcgggg gatcgattac
     7321 cctaagggaa atggggattc cggaggtcct agaaaaaaaa gggcacgagg tttacgacca
     7381 ctggagggag ggactctcca aggagaagag acaggaggtc ggcaaaaaac atcagcgggc
     7441 cgatgttttc ctgaccagca cgaacgccct tactctggac gggaaattga ttaacgtgga
     7501 tgccaccggg aaccgggttt cctccatgat cttcggccca gaaaaggtcg tggtgatctc
     7561 cggggtcaat aagatcgtca ggaatctgag cgaaggtctt gcccggatca aaaaggtcgc
     7621 tgccccgagg aactgtcaga ggcgaaagga tcccaccccc tgcgcccagg atttgatctg
     7681 ccgtaactgc aacactccgg ccaggctctg ccgggtgacg accatcattg aaagaaggcc
     7741 ttggggaatc aaagatttct cggtcatcct ggtcggagag gaacttggtt actaaagctc
     7801 gtgggggcgg ttcgcggacc gcccctcata gaattgcctc aatgaaaatc gaaagaattg
     7861 acctctgccg gcttaaaatt cctctgaaac agccctacaa gatcgccacg gcggagatga
     7921 agactttcga ttgcacaatc gtatgtcttc attcccagag gcgtgaggga ctgggagagg
     7981 ccatggcaga tattcagggc tatttttggg agaccgccga tcaggtctgg cggtttgcca
     8041 aagaacaagg cccaaagatc ctcggcctcc aggtggaaaa agcccgggat tcgatttccc
     8101 ggcatgcgaa ggaaatgccc tgctcggtga cccctttttt gaccgccctg gaaatgctga
     8161 acccgaaatc ccttcttgcc cctccttccc agcttatatc tgtgcccatg ctggggatcc
     8221 tccaggcgac ggaccccgaa gggattcgcg cggaaatcga agatttcatc tcccgtggtt
     8281 atgacaccat aaagatcaag gttggtttcg atgtggatag ggaccttgaa agggtaaaac
     8341 tggcgcaaga gatgatccaa ggaagggcgc atctgcgggc gg
//
LOCUS       DASPRR010000070         7899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1791529, whole genome shotgun sequence.
ACCESSION   DASPRR010000070 DASPRR010000000
VERSION     DASPRR010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791529"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VEL68_02475"
     CDS             complement(<1..560)
                     /locus_tag="VEL68_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34867.1"
                     /translation="MNRHSLMLLAFLVFVSLVGLSWTGTFSTYTQLVIMFMGVNIIMS
                     SSLNLINGYMGEFSCGHAGFMALGAYTSSLLTVWFFTKGSVFGPNLLPPEWSVWVFPF
                     TLLAGGVVAALAGLMVAIPSFRTRGDYLAIITLAANYIVKSAIENIQTIGGARGFMGM
                     SRVVNEMKNVLDIPWMMTWTWIFVFLT"
     gene            complement(570..1490)
                     /locus_tag="VEL68_02480"
     CDS             complement(570..1490)
                     /locus_tag="VEL68_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34868.1"
                     /translation="MFLQNMINALQWGSFYALIALGYTMVYGVLLLINFAHGDIFMVG
                     AYIAFFISTFLLGTYALQLPWTIPAGLVIFLTFLLTMALTAGVGVAIERIAYRPLRRK
                     GVSRLYVVITALMVGLILENGNLALLGASRKNFPELIPKVLYSIGDVNFTNIKIIVIA
                     VTLLVFIFLQTVVLKTKWGMAMRAISYDRVAVPLMGIPTDTIIVMTFVLGSSMAAVAG
                     ILFAVSYPVLEPYMGALVGWKAFISAVVGGIGEIRGAFVGGFILGFTEIFVAALFPST
                     FRDLIGFGVLLLILTFRPTGIFGVARRTKV"
     gene            complement(1640..2812)
                     /locus_tag="VEL68_02485"
     CDS             complement(1640..2812)
                     /locus_tag="VEL68_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ34869.1"
                     /translation="MKRQSFLTVIVLALGMFLTAPGWAAEVIKIGFDIELTGDIPKVG
                     EASKFTGEMLKEQINKQGGLKVGNKSYTLNFIYEDNEAKAESAAAAALKLITQDQVLG
                     IIGPQASKQAIPAGEVADTNQCVLISGWSTNPKTTANRPWVFRACFLDPFQGPVLAKF
                     ATEELKAKKAAVLYDIASDYPKGLAEFFKEAFEKIHGKGSVAAFETFTTKDRDFSAQL
                     TKIVASKADILFTPQYYDEVPLIVKQAHEMGWKKPILGSDSWDSAELMNLCGKDCLGS
                     FFSTHYAAPGAKGPTKQFIDQYKAKYGYEPDAVAALTWDAARLLLQAIQNTKGLTGDV
                     KKDRQAIRDQLAKLKEFEGITGKMSFDGKTGDPVKCAIIVKISPEGKFEFHKMVCP"
     gene            complement(3094..3951)
                     /locus_tag="VEL68_02490"
     CDS             complement(3094..3951)
                     /locus_tag="VEL68_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXZ34870.1"
                     /translation="MSRYVGIAGVQMEVVPEGDNSGAALRLLRDVASYFPWVDLVLFS
                     ELCVHGLNPSLKEPIPNPTIVKFCGWARKEKKWLVPGSFFEKEGKKVFNTAVVISPQG
                     KIAAQYRKIFPWRPLEKNDAGDSFCVFDIPGKGRFGLCICYDQWFPEIVRTLAWMGAE
                     VILHPTATTTSDRPQELVLSQAQAIFNQVYFLSVNGVGAGGVGKSIFVDPEGRLLQLA
                     GERETILTEVIDLDLVQRVREYGTLGLSQLWKDLKSFRGRFPIYEQGVHRGRVFKTLG
                     DLKQHRKIG"
     gene            complement(4038..5043)
                     /locus_tag="VEL68_02495"
                     /pseudo
     CDS             complement(4038..5043)
                     /locus_tag="VEL68_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(5049..6023)
                     /locus_tag="VEL68_02500"
     CDS             complement(5049..6023)
                     /locus_tag="VEL68_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34871.1"
                     /translation="MNHLLEVQDLSVSFHLLGGIVPAVAKVDLVLDEGEILGIVGESA
                     CGKSVTARSIMRIIPTPPGRINSGRVLFEGRNLMELPEEEMQEVRGEKISMIFQEPMT
                     SLNPAFTVGDQISEVFRFHRRTSKAEAWEKAVEELRRVEIPDPEKRARSYPHELSGGM
                     RQRVMIAIALACRPRILIADEPTTALDVTIQAQILKLIAQLQREIGMSVMLITHNLGI
                     VASLAQRVAVMYAGRIVEEGPAEIIFNDHHHPYTRGLLRSVPRLDLPQRDLQEIQGTV
                     PRLNDLPEGCTFSNRCDFVMDLCRRQESELRPVGEGHVSRCWLDQRVW"
     gene            complement(6115..6939)
                     /locus_tag="VEL68_02505"
     CDS             complement(6115..6939)
                     /locus_tag="VEL68_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ34872.1"
                     /translation="MFVKRFFRNKLSLFGLAIVIILVLVSFLASYIQPYDPLKISISN
                     RTQAPSKGHTFGTDHFGRDIYSRILSGAKISLTVGIVSVALGAFIGIILGAVAGYFGG
                     WIDYAITLIMDAIISFPATLLAIGLMSVMGLGVTNIIIALGFIGIPHYGRIMRGAVLS
                     IREKEYVEAARASGSGHLKLILIHVLPNAMAPLIVVTTIGVANAILIEAALSFLGLGV
                     PPPAPSWGNMLADGRNFLIQAPWMTIFPGLAISFTVLGFNTLGDGLRDVLDPKLKQ"
     gene            complement(6959..>7899)
                     /locus_tag="VEL68_02510"
     CDS             complement(6959..>7899)
                     /locus_tag="VEL68_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ34873.1"
                     /translation="VIRRLFTVIPVLWLVSVLVFSLIHLVPGDPVLVILGTTAEKAQV
                     EAMSHKLGLDRPILVQYGTWVAGLLRGDLGRSIISDEPVMEMILRRMPVTLTVAAAAL
                     LLSLSISIPTGILSALRHNTYTDYVFMGLVILAVSMPSFWLALLFILLFSVELKLLPM
                     MGYISIFTDFWEGLRYLVLPAMSLAFILAALVARMTRSSMLEVLREDYIVTALSKGTP
                     RRFIVFKHALKNAFAPVLTIIGFQVGFLLGGTVVIEDIYSIPGVGRLIFTAITNRDYP
                     VVQGCILMVTIVYVFVNAAVDLAYAYFDPRVSFDRK"
BASE COUNT         1870 a         2186 c         2254 g         1589 t
ORIGIN      
        1 gtcagaaaga caaaaatcca ggtccaggtc atcatccagg ggatatccag gacatttttc
       61 atctcattga ccacccggga catgcccata aaccctcggg ctccgccgat agtctggatg
      121 ttttcaatgg cgcttttcac aatataattg gcggcaaggg tgataatggc caaataatcg
      181 ccgcgggtgc gaaaggaggg aatcgccacc atcagcccgg ccagcgcggc gacgacccct
      241 cccgcaagga gggtaaaggg aaaaacccaa accgaccatt ccggcggcag gaggttgggt
      301 ccgaaaacag agcctttggt aaagaaccaa actgtgagga gagaggaggt gtaggccccc
      361 agggccatga atcccgcgtg cccgcaggag aattcgccca tgtagccgtt gatcagattg
      421 aggctcgagg acatgatgat attcacgccc atgaacatga tcacgagttg ggtgtacgtg
      481 ctgaaggttc cggtccagga gagacccacc aaactcacga acacaagaaa agccaaaagc
      541 atcaaggaat gcctgttcac cttggttcct tatactttgg ttctccgggc gaccccgaat
      601 atccctgtgg ggcggaaggt cagaatcaag agcaagaccc cgaacccgat cagatcgcgg
      661 aaggtggatg gaaacagagc ggcgacaaag atctccgtaa accccaggat gaatccgccg
      721 acaaaggccc cccggatctc gccgatgccg ccgacaaccg cagaaataaa ggccttccag
      781 cctaccagtg cccccatgta gggttccaga accgggtagg aaacggcaaa aaggattccg
      841 gcgacggcgg ccatggaaga gcccaataca aaagtcatga cgataatggt atccgttggg
      901 atccccatca gggggacggc gacgcggtca tacgatatgg cccgcatggc catcccccac
      961 ttggtcttga gcaccacggt ttgcaggaag ataaaaacca gcagggtaac cgcgatgacg
     1021 atgattttga tgttggtgaa attcacgtcc ccgatgctgt agagaacctt gggaatcagc
     1081 tccggaaagt tctttcggct ggctcccaga agagccaggt tcccattttc caggatcagc
     1141 ccgaccatga gcgcggtgat caccacgtaa aggcggctga cgcccttgcg gcggagggga
     1201 cgataggcaa tacgttcaat ggccaccccg acaccggcgg tgagggccat ggttaaaaga
     1261 aaggtcaaaa agataaccag gccggccgga atcgtccaag gcagttgcag ggcgtaagtg
     1321 cccagcagga aggtggagat gaaaaaagcg atgtaggcgc cgaccatgaa gatatcgccg
     1381 tgggcgaagt tgatcagcag gagaaccccg tacaccatgg tgtaccccag ggcgataagg
     1441 gcatagaaac ttccccactg cagagcattg atcatgttct gcaggaacat ggcaagggtc
     1501 aaaacttcac ctcccgggat cggagagagc gaaggcccga ggtgctcgga ttttcctctc
     1561 tcccaagcgg cttaaaaagt cggggctcaa agctcccgat tcgtctaccc gcacaggcaa
     1621 agaatttgag gttcttgttt caggggcaaa ccatcttgtg aaactcaaat ttcccttcgg
     1681 ggctgatctt aactatgatg gcgcacttga ccggatcccc ggttttgccg tcgaaggaca
     1741 ttttgccggt gatgccctca aactccttca gtttggcaag ttgatctcgg atggcttggc
     1801 ggtctttttt cacgtccccg gtcagtccct tggtattttg aatggcctga agcaaaagac
     1861 gcgccgcgtc ccaggtcaag gcggccacgg cgtccggttc atacccgtac ttggctttgt
     1921 actgatcgat aaattgcttg gtcgggcctt tagccccagg ggcggcgtag tgggtgctga
     1981 agaaactgcc caggcaatct ttcccgcaaa ggttcatgag ctcggccgaa tcccaggaat
     2041 cgctccctaa aataggtttc ttccacccca tctcatgggc ctgcttcacg atcaggggaa
     2101 cctcgtcata atattggggg gtgaagagaa tgtccgcttt ggacgccacg atcttggtca
     2161 actgggcgct gaaatcccgg tctttggtgg tgaaggtttc gaaggccgcc accgaccctt
     2221 tgccatggat cttttcaaag gcttccttga agaattccgc cagtcccttg gggtagtcgc
     2281 tggcgatatc atagagaacg gcggcctttt tggccttcag ctcctcggtg gcgaacttgg
     2341 ccagtacggg cccctggaag ggatcgagga aacaggcgcg gaaaacccag gggcggttgg
     2401 ccgtggtctt ggggttggtg gaccagccgc tgatgaggac gcactggttg gtgtccgcaa
     2461 cctcacctgc ggggatggcc tgcttgctgg cctggggccc gatgatccca agaacctgat
     2521 cctgagtgat cagcttgagg gccgccgcgg ctgccgattc ggccttggct tcgttgtcct
     2581 catagataaa attgagcgtg taggatttgt tgccaacctt taggcctccc tgcttattga
     2641 tctgttcttt cagcatctcc ccagtaaatt tagaggcctc gccgaccttg gggatatcgc
     2701 cggtaagctc gatatcgaaa ccgattttga tgacctcggc tgcccagcct ggggctgtta
     2761 ggaacattcc cagcgcgaga acgatgacgg ttaaaaacga ttgccttttc atttttttgt
     2821 tcctcctttc acgggatgga taataaaaaa accttcctaa tctttatgcg ggggatagga
     2881 agatgctgcc tatttttgca cacctgtcgg ggctcatggc cgggtctaag aaagatttga
     2941 aaggggaatg tataggatct tacccgaatt gtaaacctgt tcttatatat tgaaatccag
     3001 gagggatgtc aacaggaaat tgataaaatc aggaagcttt ctggaaagac gcttattttt
     3061 ccgcatccct tccgtatcta tttatgatcg ctcctacccg atcttccggt gttgtttcag
     3121 gtcacccagg gtcttaaaga ccctgccccg gtggaccccc tgctcataga tgggaaacct
     3181 cccgcggaaa ctctttaggt ctttccaaag ctgcgacaat cccagggtcc catattcgcg
     3241 gaccctctga accaagtcca gatcgatcac ctcggtgaga attgtctccc gctccccagc
     3301 cagctgcagg aggcgcccct ccgggtcaac gaaaatggac ttccccacgc ccccggcgcc
     3361 tactccgttc acgctaagga aatagacctg attgaaaatg gcctgggcct ggctaaggac
     3421 cagctcctgg ggacggtccg aagtggtcgt ggccgtggga tgaagaatga cctccgcccc
     3481 catccaggcc agggtgcgga cgatctccgg gaaccattga tcatagcaga tgcacaggcc
     3541 gaatcgccct tttcccggga tatcgaagac gcagaaggaa tccccggcat cattcttttc
     3601 caggggccgc cagggaaaga tctttcggta ttgggccgca atctttcctt gcggagaaat
     3661 gaccaccgcg gtgttaaaga ctttcttccc ctccttctcg aaaaaagagc cggggacgag
     3721 ccactttttt tccttacggg cccagccgca gaacttcaca atcgtcggat tgggaatggg
     3781 ttcctttaaa gaagggttca gcccatggac gcacagttcg ctgaaaagga ccaggtccac
     3841 ccaggggaaa taggaagcca cgtctctcaa caatctcaag gcagcgccgg aattatctcc
     3901 ctccgggacc acttccatct gaacgccggc aattcctaca tatcttgaca taccaatcct
     3961 ttctgtatag cgataccctg aactccccaa cccccctttt tcaagggagg gtaatcttga
     4021 ttttcagcat cccaaaatta tgacgaagca tcttcctgga cctctttggt tttggttcct
     4081 ctgaccaggc aggccaccca gtggtctggc aaaacttcct ctagcccagg ccgcagttcc
     4141 ttgcaggcat cggttttgag cgagcagcgg ggatggaagg cacaaccgcg gggactgttc
     4201 aaggggctgg gaacatcccc cgatagcact gtgaccctcc ttttcttttc cagaaccggg
     4261 tccggaatgg gaaccgccga caagagggct tcggtgtaag gatgcaaggg cttcttataa
     4321 agggattcct tgggagaaat ctccacaatc cgtccgaggt acatgaccgc gatggggtcg
     4381 cagagatatt ccaccaccgc aaggtcgtgg gagatgaaga tgagagaaag gttaagctcc
     4441 cgctgcaatt ttttcagcaa attgatgacc tgggcctgga tggacacatc cagggctgag
     4501 accggctcgt cgcaaacgat cagctccggc tgggagacca gggcccgggc cagggcgatg
     4561 cgctgtctct gccccccgga gaattcatgg gggaaacggg tcatatgttc gggcagcagc
     4621 ccaacccggc ccagggtctc ggcgacccgg tcccgtttct gccgggaatt caattccgag
     4681 aagaagttgt ccaggggttc ctggatgatc cggaagaccg acatcctcgg gtccagggag
     4741 gaataagggt cctggaagac aatctgtatc cgccggcgga atctttttag gtctggtccg
     4801 cgcagcggtg aggtcttctc cgtcaaataa cactcgccct tcggtgggtt cgatcagacg
     4861 caggatggtc ctccccgcgg tggttttacc gcagcctgac tcgcccacca gccccacgat
     4921 ctggccccgc tgcacttcca gggaaacccc gtccacggcc cgaatagaac ccccccgacc
     4981 cagggaaaac tttcggccga tgggaaagta ttttttcaga ttccggacgg aaagcaggct
     5041 catcgatttc accaaacccg ttgatccagc cagcaacggg aaacatgtcc ttcccccacc
     5101 ggccggagct ccgattcttg ccggcggcag aggtccatca cgaaatcaca ccggttggag
     5161 aaggtacatc cctccggcag gtcgttcagg cggggaaccg tcccctggat ctcctgcagg
     5221 tcccgctggg gaaggtccag ccggggaacg gaccggagaa gcccccgggt gtatggatgg
     5281 tgatggtcgt tgaaaatgat ctccgccggg ccttcttcca cgattctccc ggcgtacatg
     5341 accgccaccc gttgggccag ggaggcaacg attccaaggt tgtgggtgat gagcatgacc
     5401 gacatgccga tctctctctg gagctgggcg atgagcttaa ggatctgggc ctggatggtg
     5461 acatcaaggg ccgtggtggg ctcatcggcg attaaaatcc tcggacggca ggccaaggcg
     5521 atggcgatca tgacccgctg gcgcattccc cccgaaagct catgggggta agacctggcc
     5581 ctcttctccg ggtcggggat ctccacccgg cgcagttcct ccacggcctt ttcccacgcc
     5641 tccgccttcg aggtccgacg gtggaagcgg aaaacctcgg agatctgatc cccaacggtg
     5701 aaggccgggt tgaggctggt catgggctcc tggaagatca tggagatttt ctctccccgg
     5761 acctcctgca tctcttcttc cggcaattcc atcaggttgc gcccctcaaa aaggaccctt
     5821 cctgaattga tgcggcccgg cggagtgggg ataatccgca tgatggacct ggcggtgacc
     5881 gactttccgc aggccgattc tcccacgatc cccaggattt ctccctcgtc caggaccaaa
     5941 tcgactttgg ccactgccgg gacaatcccc cccaagaggt ggaaggaaac cgaaaggtct
     6001 tgaacttcaa gaagatgatt cattcaaaac ctattgggac gcagataacc gcggatgaac
     6061 gcggtttaag aatacggtaa atggagaaat ctgcgggtaa acaatgtttc tttttcactg
     6121 tttcaatttc gggtccagca cgtcccggag tccgtcgcca agggtattga atcccagcac
     6181 ggtgaaagaa atcgccaagc cagggaaaat ggtcatccag ggggcctgga tcaggaaatt
     6241 tcttccatcg gccagcatgt tcccccagga aggggccgga gggggaacac cgaggcccag
     6301 aaagctcagg gctgcttcga taagaatggc gttggccacc ccgatggtcg tcaccacgat
     6361 aagaggagcc atggcattgg gcagcacgtg gattaaaatg agcttcaggt gcccggaccc
     6421 tgaggcccgg gccgcctcca catactcctt ttcacggatg gaaagaactg cacccctcat
     6481 gatacgcccg taatggggga tgccgatgaa acccagggca atgatgatgt tggtcactcc
     6541 caggcccatc accgacatga gcccgatggc caggagggta gctgggaaag agatgatggc
     6601 atccatgatc agggtgatag cgtaatcgat ccaccccccg aagtagcccg cgacggctcc
     6661 caggatgatc ccaatgaagg ccccaagggc caccgaaacg atccccacgg tgagggagat
     6721 tttggcgccg gagagaatcc gggagtaaat gtcgcgcccg aagtggtccg tgccgaaggt
     6781 atggcctttc gatggggcct gggttcggtt ggagatggaa atcttgagtg ggtcataggg
     6841 ttggatgtaa ctggccaaaa aactaaccag gaccaggatg atcacgatgg ccagcccgaa
     6901 gaggctcagt ttgttgcgga agaatctttt gacgaacaaa ttaccttctc ccaattcatc
     6961 actttctatc gaaagacacc cgcgggtcga aataggcgta ggccaggtcc actgcggcgt
     7021 tcacaaagac atacacgata gtcaccatga ggatgcagcc ctggaccacg ggataatccc
     7081 gattggtgat ggcggtgaag atcagccgcc ccactccagg aatggaataa atgtcctcga
     7141 tgaccacggt ccctcccagg agaaaaccga cctggaaccc gatgatggtc aggacagggg
     7201 cgaaggcgtt cttcagggca tgcttgaaga cgataaaccg gcggggggtt cccttggaaa
     7261 gggcggtgac aatgtaatcc tcccgcaaga cctccagcat ggaagaccgg gtcatcctcg
     7321 ccaccagggc ggccaggatg aaggccagcg acatagctgg caggactaga taccggagtc
     7381 cctcccagaa gtctgtaaaa atggaaatat agcccatcat aggcaaaagc tttagctcca
     7441 cggaaaaaag caggatgaag agcagagcca gccagaagct gggcatggaa accgccagaa
     7501 tcaccaggcc catgaaaacg taatccgtat aagtgttatg gcgcagggcc gagagaatcc
     7561 cggtgggaat ggatatggaa agggagagaa gaagggccgc cgcggcgacg gtcaaggtca
     7621 cgggcatccg gcggaggatc atctccatga ccggctcgtc ggagatgatc gaacgcccga
     7681 ggtctccccg caagaggccc gcgacccagg tcccgtactg caccaggatg ggccggtcga
     7741 gacccagttt gtggctcatg gcctcgacct gagctttctc tgccgtagtc ccgaggatga
     7801 ccagaacagg gtcacccggg acgagatgga tcaggctgaa caccaggacg gaaaccagcc
     7861 acaggacggg aatgaccgtg aagagacggc ggatgacat
//
LOCUS       DASPRR010000071         6241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2276093, whole genome shotgun sequence.
ACCESSION   DASPRR010000071 DASPRR010000000
VERSION     DASPRR010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276093"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..567
                     /locus_tag="VEL68_02515"
     CDS             178..567
                     /locus_tag="VEL68_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34874.1"
                     /translation="MIRSLGGWSEVLSFRRHGERVEYDGRILGGGDFVSGILREADQK
                     VRRYLPVREKGALKDRIIKEMCKKEGVGEGELRMGGQARRVSRVRSKIAWSLSREYGI
                     SLAEIARDLGVCTSAIAKAVRRMEERD"
     gene            634..1056
                     /locus_tag="VEL68_02520"
     CDS             634..1056
                     /locus_tag="VEL68_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXZ34875.1"
                     /translation="MNRKVGRNKKKGGKKGVEPERVINLVRESLRGHIVPEFEWGAKS
                     DPEFFEKFIKLPKTCLGGGKALPAKYRLMINMCLLAHVGHLRGIPHYIKIAIEKYGAT
                     RLEIIEAFETAMLAGGVPTMIRGFAGLMSYEESKKGKS"
     gene            1458..2393
                     /locus_tag="VEL68_02525"
     CDS             1458..2393
                     /locus_tag="VEL68_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXZ34876.1"
                     /translation="MTEGERSLAVFIDFENLALGFKGKKDKRFDIQKILERLVEKGKI
                     IVKKAYADWTEYSDYKRALHEAAIELIDIPRRSVSGKNSADIRLCVDAIDLCYSKEHI
                     DTFAIVSGDSDFSPLVSKLKENGKGVIGLGMKESSSHLLINNCDEFIYYEDLERPLGI
                     PPKIEQELPERKKEGFQLLVDSVLALVRENKEVLWSSMVKETMKRKKPSFNESYHGYR
                     TFSDLLADAEKHGIIRLKTDSRSGTYVVVGFGKEEKKMEAEEPKKQKEEKERKTQKAR
                     WRPRPRKQNPNAAPAASEQAPSETAAFGLPEIEGK"
     gene            complement(2636..3661)
                     /locus_tag="VEL68_02530"
     CDS             complement(2636..3661)
                     /locus_tag="VEL68_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXZ34877.1"
                     /translation="MVQTLSREIRLAHRPIGLPRESDFELAEVRIPEPRKEEVLVRNI
                     YMSVDPYMRGRMIDRPSYVPPFQIGEPLAGACVGQVVASKSSMFQAGDYVSGAQGWRE
                     YYLSEGAALTKIDPHLAPIQTYLGALGMPGMTAYVGFLSIGLPKAGETVFVSAASGAV
                     GAVVCQIAKIKGCRVIGSAGSREKVAWLMNEARIDSAFNYKEAGDLVAEVGKHCPNGI
                     DVYYENVGGKHLEASLEHMNLFGRVVMCGMISQYNSIDPEPGPRNLFYVTTKRLTVRG
                     FIVSDHLDRRSQFLKDMSQWIAEGKIKWRETIVAGIENAPKAFIALFKGENFGKMLVK
                     IGPDPAV"
     gene            complement(3854..5785)
                     /locus_tag="VEL68_02535"
     CDS             complement(3854..5785)
                     /locus_tag="VEL68_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ34878.1"
                     /translation="MPNGYHGKILHVNLTLRTLTTEEPSEAFYRSYMGGSALNLHYLL
                     KEMPPGVDPLGPDNILGLSVGVATGVSISGQSRMTATAKSPLTGAIGDSQCGGFWPAE
                     LKFAGFDGIIIRGKASSPVYLWVHDGKAEIRDASHLWGRVTGDGEKAIREELGDQKVE
                     VMQIGPAGEKLVRFACLINMCNRANGRTGMGAVMGSKNLKAVAVRGKQRPEVAEKESL
                     AKLRQWATEVFPKSGVSGLGKYGTPGVVSVQQNTGGLPTLNFTSGVFEGWKTIDGHNL
                     YDTALKGRETGKQDQEGRDTCFGCIIRCKRVVEIKEGPYQVDPLYGGPEYETVATFGS
                     YCGVDDLPATCKANEICNRLGMDTISCGATIAWAMEAFEAGAITPKDTGGLNLRFGNA
                     EAMVRLTEMIGKREGFGNLLAEGSARAAEQLGRGKEYLITSKGQEAPAHMPQVKRSLA
                     LIYSVNPFGADHQSSDHDPSYEAAFEYFKNRLGTLGLTHPQEPRSLGPEKIKFARKTQ
                     YFCSMLDSLNLCQFACGASWQLYGPEEIVKMLQAITGWNVTIDELLDVGERRLNMMRA
                     FNAREGIGRTQDTLSEKFFQKTLKGGPSDGWKIDKAEWEAARDEYYRQSGWDVKMGTP
                     TRQTLERLGLGWVANKVGL"
BASE COUNT         1468 a         1640 c         1715 g         1418 t
ORIGIN      
        1 cggtatcctt ggagtggcca tagggttttg gtggcaagag aggagaatgt ttggcaggag
       61 aggaaatatg tgcttcggca attccacccc agagaaaagg aggcgattcg gatctaccgg
      121 aggtttatcg aggaggggaa ggatcgaggg cggcggccgg atttagttgg aggaggaatg
      181 atacggagtt tggggggatg gtcggaggtt ttgtcattta gaaggcatgg ggaaagagtc
      241 gagtatgacg ggaggatatt ggggggaggg gattttgttt cggggatatt gcgggaggcg
      301 gaccagaagg taaggaggta tcttcctgtc cgggaaaagg gagcgttgaa agatcgaatc
      361 attaaagaaa tgtgcaagaa agaaggggtt ggggagggag agctgaggat gggagggcag
      421 gcgaggaggg tttcgcgagt ccgatccaag attgcctgga gcttgagccg ggagtatggg
      481 atttcgctgg cagagatcgc ccgggactta ggggtgtgca cctccgccat cgccaaggcg
      541 gttcgaagaa tggaggaaag agattaaagg tgcaaattct ccactgcgtt ccctaatccc
      601 ggatatctta tttccatgcg gaggtgtaga gaaatgaata gaaaagtagg aagaaacaaa
      661 aagaagggtg ggaaaaaagg ggtagagccc gagagggtta tcaatctcgt gcgagaaagc
      721 ctacgtgggc atattgtccc agaatttgag tggggcgcaa aatccgatcc ggaatttttt
      781 gagaagttta taaaactgcc gaaaacctgc ctgggaggcg gaaaagccct ccccgctaaa
      841 tatcggctaa tgatcaacat gtgcttgttg gcccatgtag gtcatttacg cgggattccc
      901 cattacatca agatcgcaat tgagaaatac ggggcaacaa gattagaaat tatcgaggcc
      961 tttgaaacgg caatgctcgc agggggggtt ccgacgatga ttaggggatt cgcgggactc
     1021 atgagctatg aagaaagcaa gaagggaaaa tcctagccag tggggggccg gggaatcacg
     1081 atcttttcca ccaggttaga tctgtgggta caacaggaat gatggtgttt tctaagaaaa
     1141 ggacagactc aaaatctgtt aatgggggac gttcgttcaa agacaactgg tgatgaatcg
     1201 ggcgccggtg ttgacgcttt gggcggcggg tgaagggaga gagggacctt gtccaattca
     1261 ctcattttat tttccccttt tctctttcct catccccgct gaaaacgccc cccaaacaaa
     1321 tccaagatct tgtgctaaaa tcccttgacc ccaaattccg tcttttgtat attcaaccca
     1381 ccaaagcaag ttcttatcaa tttcaggatg gtctcggggg cagagtaaaa ttcacgaaac
     1441 ctggaaagga cgagcagatg actgaaggag aacgatccct ggcggtcttc atcgactttg
     1501 aaaacctcgc cctggggttc aagggcaaaa aagacaaacg cttcgatatc cagaaaatcc
     1561 tcgaacgcct ggttgaaaaa gggaagatca ttgtgaagaa ggcctatgcc gattggaccg
     1621 agtattccga ttataaacga gcccttcacg aggcggcgat tgagctgatc gatattccca
     1681 gacggtccgt ttccgggaag aactcggccg acatccggct ctgcgtggat gccatcgatc
     1741 tctgctactc caaggagcat attgacacct tcgccatcgt ttccggggat agtgattttt
     1801 ctcccctcgt ctcaaagctt aaggagaacg gcaaaggggt tatcggtctc gggatgaaag
     1861 aatcaagttc ccacctcctg atcaataact gcgacgagtt catctattac gaggaccttg
     1921 agcggcccct ggggattcct ccgaagatcg aacaggaact gcctgagcga aagaaggaag
     1981 gatttcaact tctagttgac tcggtgctgg ctctggttcg ggaaaacaag gaagtgctct
     2041 ggtcttctat ggtcaaggaa acgatgaagc gaaagaaacc ctccttcaac gaatcctacc
     2101 atggttatcg gacattcagc gatcttcttg ccgatgcgga aaaacacggg atcattcggc
     2161 ttaaaaccga ttcacgaagc gggacctatg tggtcgtcgg tttcggcaaa gaggagaaaa
     2221 aaatggaggc tgaggagccc aaaaaacaga aagaggagaa agaacggaaa acccagaagg
     2281 cgcgctggcg tccgcggcca agaaagcaga atcccaacgc cgctcctgcc gcttctgaac
     2341 aggctccttc tgaaacagct gcttttgggc ttccagaaat tgaaggaaag tagcagtggc
     2401 aagggtcgcg gtagggatgg tggttgccca gcgcccctgg ccagaaatgg gccatataaa
     2461 ccaacccaag aacgaccatt ttccggcggc aaaattggtc caggccctga atgaattctg
     2521 cagcaaaatc cccccaaata cccggtgccg tatcgaggtc cgcaatgaag catatttggc
     2581 ggatcgtggt ttcgaaatct tgaaaaagca tgggataggg tcggtatgtt ccctttcaga
     2641 ccgctggatc cgggccaatc ttaacgagca tcttgccgaa attctcgcct ttgaataggg
     2701 caatgaatgc cttcggtgcg ttctcgatcc cggcgacgat cgtctccctc cacttgatct
     2761 tcccttcggc gatccactgg ctcatgtcct tcaagaactg cgaccgcctg tcgagatggt
     2821 cggaaacgat aaatccccgg accgtcaatc gcttcgtcgt cacataaaat aagttgcgcg
     2881 gtccgggttc cggatcgata gaattgtact gggaaatcat gccgcacatg accacgcgcc
     2941 caaagaggtt catatgctct aaggacgctt cgagatgctt gccgccgacg ttttcatagt
     3001 aaacgtcaat cccgttagga cagtgtttgc ccacctcggc aacgagatcg cccgcttctt
     3061 tgtagttaaa ggcactatcg attctggctt cattcatcaa ccaggcgacc ttttccctcg
     3121 acccggcact tccgataacc cggcagccct tgatcttggc aatctggcac acaaccgcgc
     3181 ccacggcacc ggacgctgcg gagacaaaga cggtctcgcc tgccttggga agcccgatgg
     3241 agagaaatcc gacatatgcc gtcattcccg gcataccaag ggcgccaagg tacgtctgga
     3301 tcggcgcaag gtgcggatca attttggtca gggctgctcc ttccgacagg taatactcgc
     3361 gccatccctg ggcgccggag acatagtcgc cagcctgaaa catggagctc ttggatgcca
     3421 ccacctgccc aacacaagcg ccggccagcg gctcgccgat ctgaaacggg gggacatagc
     3481 tcgggcggtc aatcatacgt ccgcgcatgt aaggatcgac cgacatgtag atattgcgca
     3541 caagcacctc ttcctttctg ggttcaggaa tgcgcacttc ggccagttcg aagtcgcttt
     3601 ccctcggtag gccgatgggc cggtgggcaa ggcggatttc acggctcaga gtttgcacga
     3661 ttttccctcc ttcacgtttt tccgacctcg ctcggaggtc agagattgat gcttttccga
     3721 ttattctgtg ctttttaaga gaatcttgcc agggcatttc gctcagagat gggaaagccc
     3781 ctaagggaag caggggaggg gacggaagca ttgaagggtg gtttatccgt ggccgtttgg
     3841 gcaacgataa ggtttatagc ccgaccttgt tggccaccca tcccagtccc aatcgttcca
     3901 gcgtttgacg ggtcggtgtc cccattttga cgtcccaacc ggactggcgg tagtactcat
     3961 cccgggcggc ctcccattcg gccttgtcga tcttccagcc gtcgctcggc ccgcccttga
     4021 gggtcttttg gaagaatttc tcgctcaggg tgtcctgggt ccgaccgatg ccttcccggg
     4081 cattgaaggc acgcatcata ttcaaccggc gttcgcccac gtccaggagt tcatcgatcg
     4141 ttacattcca gcccgtgatc gcctgcaaca tcttcacgat ttcctctgga ccataaagct
     4201 gccaggacgc gccgcaggca aactggcata ggttgaggct atccagcata ctgcaaaaat
     4261 actgcgtctt acgagcaaat tttatttttt ccggccctag gctgcggggc tcctgaggat
     4321 gggtcaggcc cagggtcccc agtcgattct tgaagtattc aaaagccgcc tcgtaagatg
     4381 gatcatggtc actgctctga tggtcggcgc caaatgggtt aaccgagtaa atcaaagcaa
     4441 ggcttcgttt tacctggggc atatgggccg gtgcttcctg ccccttgctc gtgatgagat
     4501 attctttccc cctccccaac tgctccgctg cacgcgccga accctcggcc aacaggtttc
     4561 caaagccctc tcgttttccg atcatctcgg tgagcctcac catggcctcg gcattgccaa
     4621 acctcaggtt aagccctccc gtgtcctttg gagtgatcgc cccggcttcg aaggcttcca
     4681 tagcccaggc tatggttgcg ccgcaggaga tcgtgtccat tcccaaccta ttgcagattt
     4741 cattggcttt gcaggttgca ggtagatcat cgacaccgca gtaggagcca aaggtggcaa
     4801 ccgtttcata ctccggaccg ccataaagag ggtctacctg gtaaggtcct tctttgattt
     4861 ccacgacgcg cttgcagcgg atgatacatc caaagcaagt gtcccggccc tcctggtcct
     4921 gttttcccgt ttcccggccc ttgagagccg tgtcgtacag gttgtgcccg tcgattgtct
     4981 tccagccctc gaaaacgccg ctggtgaaat ttaaagtggg cagcccccct gtgttctgtt
     5041 gcacggagac cacgccggga gtgccgtatt ttcccagccc gctgacgcct gattttggaa
     5101 aaacctccgt tgcccactgt ctcaacttag ccagggactc cttttctgca acctccggcc
     5161 tctgtttgcc ccgcacggcg acagctttga ggttcttgct ccccatgacg gcccccatgc
     5221 ccgtgcgtcc attcgcccgg ttgcacatat tgatcagaca ggcaaaccga actaactttt
     5281 caccggcggg gccaatctgc atgacctcca ccttctgatc cccaagttcc tctcggatcg
     5341 ccttttcgcc gtcgcctgta accctgcccc agaggtgaga agcatcccga atttcggctt
     5401 tcccgtcatg gacccacagg taaacggggg acgatgcctt cccccggata atgattccat
     5461 cgaaaccggc aaatttcaac tccgccggcc agaatcctcc gcattgcgaa tccccaatag
     5521 cgcccgtaag gggcgatttg gcggtcgccg tcatccggct ttggccggaa atggatacgc
     5581 cggtggccac ccccacgctg aggccaagga tgttgtcggg cccaaggggg tcaacgcccg
     5641 gaggcatttc cttcaaaaga tagtggagat tgagtgcact tcctcccatg tagctgcggt
     5701 aaaaagcctc agacggctct tccgtcgtga gcgttcgaag cgttaggttc acatgcagga
     5761 tcttcccgtg gtatccgttt ggcatagctt cctccttgaa ggacaagact tgcttcagga
     5821 aggggggctt acttccccgc cttcaaacgg cggagctgaa tcccaccgta tggttcaact
     5881 ttaagaaaaa ggaaggtgtc tgtcaatgag gaatgcccct cccaaagtcc ctaacgccat
     5941 cggaaaggcc ctttctaaaa aaggggggcg gtcaaaggcc tcctggtctt ctttgctcaa
     6001 agcccggcga aatggtttcc acctttcccg ttcggattcc agaacatgac tgaaaggcat
     6061 aactgtccgg cccatttttc cctcccagag attggggggg aataatcagg tcgtgcccct
     6121 agaatccccc aaagagtcca gaatactcat catttgatga ggtgtcaaga ggaatccttc
     6181 cgggtagtgg atgtgaggat gttaactccc ttgcgaagag ggttatttga cttctcctga
     6241 c
//
LOCUS       DASPRR010000072        24952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_333194, whole genome shotgun sequence.
ACCESSION   DASPRR010000072 DASPRR010000000
VERSION     DASPRR010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 24952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24952)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24952
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333194"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..1032)
                     /locus_tag="VEL68_02540"
     CDS             complement(394..1032)
                     /locus_tag="VEL68_02540"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXZ34879.1"
                     /translation="MTCPIFKANPFGIKKAAQIILQGGVIAFPTETFYGLAADSRNEA
                     AIKKVFQVKGREEGNPILLLVVDRSWLSGLVQEVYPSAERLMEKFWPGPLTMVFRASP
                     NLSPLLTANTGKIGIRAPGLALARALTRAVGRPITGTSANLSGRPSPSTAEEVVQSLG
                     EKLDAILDGGRTAGGLGTTVLDVSGPSFRVLRDGVVSKKDLEPFFIELASTN"
     gene            complement(1102..2802)
                     /locus_tag="VEL68_02545"
     CDS             complement(1102..2802)
                     /locus_tag="VEL68_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ34880.1"
                     /translation="MVQTPWLKYYEDGVPPSLEYPKVSLNHFLEQSAASFPQSVAMIF
                     AGKKFTYGETQGKVQALASAFYGLGVRKGDRVGLLLPNSPPYIIGYYATLKIGAIVVS
                     LNPLSVERELLHLLKHSGVRTLIVAEPLFPRIAEIAAQSSLENILIACLRQWADRKKP
                     SERSQFFPSPQKRKGIYLLEPLLEKYAARKVPVVSLEPEDEGVLQYTGAISEGIKGVV
                     LTHANLVANTLQISSWVVRARRGKEVLISVLPFFHVYGMAVAMNVPIYLAATMIIMPR
                     FEVMGTLQAIKRYRPTFFPGVPTMFVALSQEKNAEKYNLSSLRVCYSGAGPLAVDTLE
                     DFEKLTGARVTEGYGLAEASPATHCNPIFGKRKLGSIGLPYPDTLAKIVDLEAGQKEL
                     SPGEVGELCIQGPQVMKGYWKGPEETARTIRNGWLYTGDIARMDEDGYFFILDIKKDM
                     IIAGGFNIYPKDIDQVLAEHPKVAQAIAVGIPDRYRGETVKAFIVLKTGHSATQDEIL
                     DFCRKNLAKYKVPAQVEFRASLPKASSNKILRRLLRQQVIEEEKKKAGIKGSRILGFE
                     "
     gene            complement(2876..3379)
                     /gene="purE"
                     /locus_tag="VEL68_02550"
     CDS             complement(2876..3379)
                     /gene="purE"
                     /locus_tag="VEL68_02550"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXZ34881.1"
                     /translation="MENQKPLVAILMGSDSDFPVMEEAARILTEFQVPYEITVSSAHR
                     SPDRTAEFARRAERRGVEVIIAGAGGAAHLAGVIAAHTILPVIGVPMESGFMGLDSLL
                     STVQMPGGVPVATVAIGKAGARNAGILAVQILSGNHRELKKSLVRYKASLVAKVEEAA
                     KKIKRQP"
     gene            complement(3387..4670)
                     /gene="purD"
                     /locus_tag="VEL68_02555"
     CDS             complement(3387..4670)
                     /gene="purD"
                     /locus_tag="VEL68_02555"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXZ34882.1"
                     /translation="MKVLVIGGGGREHALVWKISQSPRVKKIYCAPGNAGIAQLAECL
                     SVSAEDVKGLTARAEKERVDLTVVGPEAPLTLGIVEAFKEKGMRIFGPSQKAAEIEGS
                     KAFAKDLMKKYGIPTGDSQTFTDHGPAVKYAKGKGAPLVVKADGLAAGKGVIICRSVD
                     EALSALDLIMVQKAFGAAGGKVVVEEFLEGEEASFLAFTDGEAVLPLPTSQDHKAIYD
                     KDQGPNTGGMGAYSPAPVVNKRVHREVMEKVMSPTVRGMAKEGRKYQGVLYAGLMIKE
                     EKTKVLEFNARLGDPEAQPLLMRMKSDLVPVLEATIDGTLAQQKIEWEDRASVCVVIA
                     SGGYPGSYEKGKVISGLQEAAKVRDTFVFHAGTTLKEGKVVTNGGRVLGVTALGSGIQ
                     EAIQRAYEAVAKISWEGAYYRKDIGQKALQRMGKR"
     gene            complement(4906..5484)
                     /gene="purH"
                     /locus_tag="VEL68_02560"
                     /pseudo
     CDS             complement(4906..5484)
                     /gene="purH"
                     /locus_tag="VEL68_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="involved in de novo purine biosynthesis;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
     gene            complement(5582..6628)
                     /gene="selD"
                     /locus_tag="VEL68_02565"
     CDS             complement(5582..6628)
                     /gene="selD"
                     /locus_tag="VEL68_02565"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6581..6583),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXZ34883.1"
                     /translation="MNKKVRLTAMVKAGGUAAKLSPEDLDKALKPLPLERHPNLLVGT
                     ETADDAGVYKISEEMALVQTVDFITPIVDDPFLFGQISAANSLSDIYAMGGKPLTAMN
                     VIGFPRPILDVSILTEILRGGLEKVHEAGAVLMGGHTVEDVELKYGLSVTGTVHPRKI
                     LTNRAARAGDVLVLTKPIGTGMISTANKAEIVEEEFLRKAIDSMARLNDKAAEVMALC
                     HAHACTDITGFGLLGHSSEMARGSGLSFHLRYSLIPLLPGAREYAAMGMVPAGAYCNQ
                     THFGPKISFAHAVPELERVILFDPQTSGGLLIALPSEEGEKLLGLCRQRGITEVALVG
                     EVVPPEKSLITVEY"
     gene            complement(6648..8093)
                     /locus_tag="VEL68_02570"
     CDS             complement(6648..8093)
                     /locus_tag="VEL68_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34884.1"
                     /translation="MGSWVSGCATLNPGPRRENFAPLFLYSEDEEREGKGIDILGPFF
                     TYRRDSEEKDVAFRPLFYSKTKPAQYRLDYLYPMGKYERTDRRVESYFRLIYSTDRDL
                     EKEKPEKKERGFLLAFWGETEKGEPYGGFFPLYGSLKNRFGRDEMNFFLWPIYSDSRE
                     GDNKTYTFLWPFFSYSEGTGREGFKFWPLGGYDRKENDYQKTFFLWPFFHFEKRYLYT
                     DDPTEINMVFPLYVSETSSRRAKRSVLWPLFTHVRDDDDHYTQWDVPWPLVQWAKGDE
                     KEIFRVFPVYGRKYWESYERGYILWPIYWYVHDKGDNYRRTLDRYLLLSKNETKIWEK
                     EGKKERRLRVWPFFYWRQETDGSEYLYWPCLIPVDYEGYEKNWVPLLSLYEYRRNPQG
                     ASESKFLWGVYVHRQNQVRDLYELSFLFTLYTTEDLLYFSILKGLVEYLADGGKRALR
                     LAYSPRPIEWENFSASREALAFWGGIAQITE"
     gene            complement(8162..9355)
                     /locus_tag="VEL68_02575"
     CDS             complement(8162..9355)
                     /locus_tag="VEL68_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXZ34885.1"
                     /translation="MKSIATETKVGIFVLLGIVLLTLFTVRVGRIAIREEGYRTYSYV
                     ESAAGLEKNSPVRIAGVEVGKVEGIKLEGLKAKVTMLLPFNVKLPLGSKAYVKSAGLL
                     GEKYVEIVPGTGTEIIKADGLVEEGGPSADVDRVLTQLSSVGGDIQRITRSLSNVLGG
                     EEGERSIKELVTGAKETMTNLQHITDSVNRGEGTLGKLVKDDKLYAEVKDTMANLREV
                     SQSIEEGKGTLGKLVKDEALYVETKATMIEAKETLANLNKVSKQIESGEGTLGKLVKD
                     EALYNETKATMSEAKEALASLKKVSDHIEKGEGTLGKLVMDDSLFNDAKAAMTEAKET
                     MTNLKKVSEQIEKGEGTLGKLAKDDALYDDTKRAVKSVQKAADGITEVTPVTVLGVLL
                     GTVIR"
     gene            complement(9376..10197)
                     /locus_tag="VEL68_02580"
     CDS             complement(9376..10197)
                     /locus_tag="VEL68_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34886.1"
                     /translation="MAEDPEGEKKSCRNPDDPMIVVKELYKSFGPNHVLRGANLEVKC
                     GESMVVLGGSGTGKSVLIKCIIGLLRHDRGEIYVDGTEISDLDEEEWNELRKKFGMLF
                     QRDALFDSLNVWENVGFGLRQHTKLPDDEIKAAAVEKLKLVGLKNIENLMPSELSGGM
                     RKRVSLARAIAMEPAILLYDEPTTGLDPIMANVINELIVEMREKLDVTSIAITHDMIS
                     AYRIADRIAMLYKGQIIEVGTPGEIKASPNEIVQQFIHGQVEGPITRGTDIGRKL"
     gene            complement(10340..11110)
                     /locus_tag="VEL68_02585"
     CDS             complement(10340..11110)
                     /locus_tag="VEL68_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ34887.1"
                     /translation="MFSLFDIIGFRVLRFLAESGKVMIFSFKTLTWTFRPPFDIRNLL
                     KQMEEVGIKSVPVVLITGAFTGMVLALQSYTGFKRFNAEAFVGTVVALSMTRELGPVL
                     SGLMVSGRVGSAMAAELGTMQVTEQIDALYTLATNPIKYLIVPRFLASVIIMPVLTLF
                     ADLVGILGGYLVSVKLLGSNPTIYLRRTFDYLDLEDIYIGLLKSLVFGMIIAIIGCYQ
                     GFHTEGGAEGVGKATTKAVVMSSLLILIANYFITALFI"
     gene            complement(11148..11327)
                     /locus_tag="VEL68_02590"
     CDS             complement(11148..11327)
                     /locus_tag="VEL68_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ34888.1"
                     /translation="MDLTIVDVSEIPGVQVGDEVVLLEEITAAEMAGWIESIPYEVLC
                     SIGKRVPRVYQRSEG"
     gene            complement(11442..13616)
                     /locus_tag="VEL68_02595"
     CDS             complement(11442..13616)
                     /locus_tag="VEL68_02595"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXZ34889.1"
                     /translation="MGIYHLDPYFLSILELKTLDARFLSRGKIPTSNQVVLVTIDEKS
                     LDELGRWPWTRVRMAQVLDALVKAEVKVVGFDIVWAEPDENSQLKVLTEIKQKLTELK
                     VANPNLERYIAEALQEADSDRILADSLARSQRAVLGYFFHFSPRESSGGQEGPAKEMP
                     PLTSFNLVKFTSEEAARVPVFEAVHAEVNIPVISQAAEGAGYFNIFPDPDGTVRWAPL
                     VIKYQDRFYSSLSLAVLQKFQDNSPLALRIAEYGVDQVRLGKVSIPANEEGRLLINYR
                     GPQKTFPHYSATDVIHGRVPEKAFQGKIALVGATAIGIYDMRVTPFEHVYPGLEIHAN
                     VIDSILQNQFLYRPNWATLVDILVIAVVGLILGGLLPRFKAHWGALVGGALFLSVLAL
                     GQYLFETQGVWMNLTYPSLNLVFIYLGVTGYRYMTEEKEKKRIRGAFQYYLTASVVEE
                     MLRNPDKLKLGGEKKDLTVLFSDIRGFTSISERMTPDGLVKFLNEYLTKMTDVVFKYD
                     GLLDKYIGDAVMAVWGAPLDQPDHARRACLTALDMLDELHRLQKKWSAEGLPVLNIGI
                     GVNAGPMVVGNMGSDRRFNYTVMGDSVNTGSRLEGLNKVYGTNIIVSQTTLEKVQEEF
                     IARELDLVRVKGKGQPVKIFELLSLVKTASTDQRALADGFHAALAEYRKRNWDKAREA
                     FQKVLAQFPHDGPAKLYLERSETLSKNPPPADWDGVYTMTTK"
     gene            14285..14359
                     /locus_tag="VEL68_02600"
     tRNA            14285..14359
                     /locus_tag="VEL68_02600"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14317..14319,aa:Thr,seq:cgt)
     gene            15009..16079
                     /locus_tag="VEL68_02605"
     CDS             15009..16079
                     /locus_tag="VEL68_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXZ34890.1"
                     /translation="MEEDKSGVDKFMNIDKFITELIGYHEQIKQLKASEVHYKDLVGT
                     LQDSLNKYRAVIDHLPQKVFLKDKNLFYLFGNQNYARFLGVDPQKIPGKTDHDFFPYE
                     KAEQYLNDDRKFMEQGHPEEKEERATRDGKERVEQVAKYPVRNEAGEVVGILGISWDI
                     TDKKEREEDLERRSLELTRLLEVRTEEWKEIGGKFQSEQAERRRLEERLKSLEGYYRI
                     LFENTGTAVAVVEDNKVLSRVNTEFERFSGYSRAEVEGAKNWNEFIHNGSPQDMGKAT
                     GSPDMSSPDPGMQVFKFVDKQNRERTVSMTAARIPDTNRVMVSLMDITKYKQAREELN
                     RIMKQFMELMVEMERGVKNLDG"
     gene            16201..17163
                     /locus_tag="VEL68_02610"
     CDS             16201..17163
                     /locus_tag="VEL68_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ34891.1"
                     /translation="MNFFRKKKKLLNPFLCDPKLKFVLQENTYESFYSFLKDPFASHP
                     DPNLFFLTDNCREVWNSVLQGIFERKGFILLTGENGIGKTTLMALVYLFLTTNGGKVK
                     VIPLFDPPNAIEEILRAVLRNLGFPAEEENKSAMLSLLDEDILQRSAQGETVAMIFDE
                     AQNLRKEVLEEIRLFASPHPRRPKFLQEIFVGAPQFEKKLNGRDLLVLNQRFEVRCRL
                     RPFTLEESLGYIEHRLNRVGSTTSKVFTPRAVYLIACHAKGIPGKLNRVCQETLSVAY
                     TQMKEKVDSANVREALANLGMVRKELWQLREKTLSWFKRNSASL"
     gene            17856..20450
                     /locus_tag="VEL68_02615"
     CDS             17856..20450
                     /locus_tag="VEL68_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXZ34892.1"
                     /translation="MNFGKTTPQGADNFEFIFENAPQGIYLLTSDWKFLTVNPAMAHI
                     LGYESPSDLIASFTDPHRQYYVSSERNAKFRELVTVQGMVRDFEAQVFRKDGSIIWIS
                     TSARRIQDPEGKLLYVEGLATDITEAKQFEESSKDLEEQNRLLIQHSNDAIFIAQDEV
                     IKFANPKAEELFGYSAAEMAKIPFGNHIHPDDREMVLEKHRRRLEGQPIPAISPFRII
                     NRSGQELCIETNAVVIEWGKKPATLMIARNLSEERKEEVELGKAHNMYRAILGNINDI
                     VLSLDDKGCLTYINPVVEKKLSYKMEEMTGKPLSSFIHPHAEPDFRENMKRCLSGAQR
                     TFEFRILDKKNQIFDMRFSGRPLVKDGQTIGLTGILMDITEQELSATLLNRAERNYRS
                     IFENALEGIFQSTIDGHFLVANPACARILGYSSPEELITRHLSPDRPFYANPEIRKEF
                     QTLLQEVGVVKGFEYEVYRKDGEKIWVSENALAMRDSNGNVLFYEGTLQDITDKKQAE
                     DKIRFLSFYDSLTGVYNRAFFEENLRRLDSPRQLPISILMGDANGLKLINDAFGHQEG
                     DRFLQEIAAILKSCCRKEDMVARLGGDEFAIMLPRTNHGVSMEITERIKKICKEKSSA
                     PFEVSMALGVATKVDPSKSIEVVLKEAEDRMYRNKLLESKSFRTSLLSSLRQTLFEKS
                     HETEEHTRRLQRLALQIGNALKLPDSILDELTLLANLHDIGKIAIPEEIIIKPDRLSL
                     EEWDLIRKHPEIGYRIANSSPEMVPVAEGILSHHEWWNGAGYPRMLFGKEIPLASRII
                     SVVDAYDVMIYGRPYKKALSKQEALAEIKRCAGSQFDPLISQVFLKILSGAKLGEIIA
                     SQLIPDVL"
     gene            complement(20597..22489)
                     /gene="metG"
                     /locus_tag="VEL68_02620"
     CDS             complement(20597..22489)
                     /gene="metG"
                     /locus_tag="VEL68_02620"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXZ34893.1"
                     /translation="MKRIYFTTPIYYVNAEPHIGHAYTTIVADVLNRFYKLAGYDTFF
                     LTGTDEHGDKVAQAARAAGETPQAYADRISGLFRALWPKLNITHDRFIRTTEEAHKKV
                     VQHILQKVYDAGDIYFGEYGGHYCRGCERFLTEKELIEGKCPDHGTAPDFIQEKNYFF
                     RMSKYQGWLIEHIQANPDFIRPERYRNEVLGFLRDPLEDLCISRPKSRLEWGIPLPFD
                     SNYVTYVWFDALINYLTGVDYPDGERYGHLWPVCQHLIAKDILKPHGIYWPTMLKSAG
                     VPQYQHLNVHGYWIVSDSKMSKSRGNIVKPLDLKDKYGLDAFRYFLLREMAFGLDSNF
                     SETALVGRINADLANDLGNLVSRTLAMAERFRGGVIPRPAGLEDKDIELKDLAMRIAR
                     EAEPTMEQLGFHKLLISTWELINLANKYIDGMAPWALAKDPQKAERLDTVLYQLLEVL
                     RFSAVLISPFMPQTAEKIQAYLGISEPDRQNLASLEEWGGLKPGGQVRKGESLFPRIE
                     EAPKREEKEKMETSNIITYEEFQKLDLRAAKVLAAENIKKSDKLLKLRIDIGEERTLV
                     AGIAKAYKPEELVGKTIIIVANLQPTKLMGVESQGMLLAADGADDTGVVLATFDKEAK
                     VGAKVR"
     gene            complement(22486..23310)
                     /locus_tag="VEL68_02625"
     CDS             complement(22486..23310)
                     /locus_tag="VEL68_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HXZ34894.1"
                     /translation="MKVVGVKFRDHGRIYDYDSTGFTLKERDIVVVDTERGPEIGFVA
                     RMPLERDPKYFTKTLKKVLRLADQQDMEQGRHNLIREREAKKVCLAKIQEHKLPMKLI
                     GVESFLDGSKIIFYFVSEGRVDFRALVKDLASAFKTRIEMRQVGVRNEAKMIGGLGTC
                     GREFCCCAFLKEFEPVSVKMAKEQNLALNPQKISGACGRLMCCLAYEVDTYSELKKDL
                     PKVGKRVITPQGPGKVMQQSIINQKLKVTLDDGKEVEVGFDEVREESFFDRVRKGK"
     gene            complement(23436..24443)
                     /gene="holB"
                     /locus_tag="VEL68_02630"
     CDS             complement(23436..24443)
                     /gene="holB"
                     /locus_tag="VEL68_02630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXZ34895.1"
                     /translation="MSFDQIRGQEKAIAILRNALGNDRLAHAYLFIGPEGVGKHLTAL
                     TLAKAMNCQSPPEAGDCCEKCTSCIKVNSSNHADVIAVEPEGEFIKIDQVREIQKRLR
                     FRPMEGGRRACIIDPADQMKAEASNALLKILEEPPRDTHLLLITARPHQLLSTILSRC
                     QWVKFKPLSQPDIAQILKKDKGLDEEKARFYASLAGGSAGQAVSLSARVDFQKRLDWL
                     QVFSDLPQKSTEEVFETCERLAKEEEEIGDLMDLWKIWVRDLTVFKVQGDRTKEGLIN
                     HDLLSTVEKDAAKSTFDRLEGIFSLISEVQRSLAYNVNKQLALETLMLEIRKERPMER
                     G"
     gene            complement(24447..>24952)
                     /gene="tmk"
                     /locus_tag="VEL68_02635"
     CDS             complement(24447..>24952)
                     /gene="tmk"
                     /locus_tag="VEL68_02635"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXZ34896.1"
                     /translation="GEKIRQILLSSDNAGMEPGTELFLYIADRIQHYHQVIAPALQEG
                     KAVLCDRFADATTVYQGFGRGLDLAWIEEIHARALENIKPDLTFLLDLPVEVGLRRAW
                     KRLENDNTQEDRFEKEALDFHQRIREGYLTLARKEPGRIIVLDGKKDEAALHREIVSH
                     LPGSLRG"
BASE COUNT         5558 a         6890 c         6495 g         6009 t
ORIGIN      
        1 ggagatgcag gaggacgggt ggtggcctgg ggccggcccg aagaagtcgc caggtcactc
       61 aactcccgga cggcttctta cctgcgggag tacctgagaa gacaaaatag aaatacggaa
      121 gaacggaggc agaaaagggc ataggagatt tctgggggcg gtgtagccta gagttactga
      181 ggagtccata attgagaagg catatctgtg cgaaactcta ctcctacccc gatgcttttc
      241 gcttagcctg ttaccgttca ccctgagctt gtcgaagggt gcgtcactaa gccgttcatg
      301 cttcgacaaa ctcagcacga acggcctagt cttcagcgtc catacaatta tggactgatt
      361 agtaagaaag ggagccccct ttttcgattc cggtcaattc gtcgatgcaa gctcaatgaa
      421 aaatggttcg aggtcttttt tgctgacgac cccatcccgg aggacccgga aggatgggcc
      481 cgatacatcc agcacagtgg tccccaggcc ccctgcagtc ctgcccccgt cgagaatcgc
      541 atccagcttt tcccccaggc tctgaaccac ttcctcggca gtcgaggggc tgggccgacc
      601 ggaaagattg gcactggttc cggtgatggg ccggcccacg gcacgggtca gagcccgggc
      661 cagcgcaagg cccggagctc gaatgccgat ctttccggtg ttggcggtca aaagaggaga
      721 gaggttggga gaagcccgaa agaccatggt gagggggccg ggccagaatt tctccatcaa
      781 ccgttctgcc gagggataca cttcttgcac caatccactt agccagctcc gatcgaccac
      841 cagaagcaga atcgggttac cttcctcccg gccttttact tgaaagactt ttttaatcgc
      901 cgcttcattc cgggaatccg ccgccagacc gtagaaagtc tcggtaggaa aggctataac
      961 ccctccctga aggatgattt gggcggcttt tttgattcca aagggattgg ctttgaagat
     1021 ggggcaggtc atgtgaagaa caaatatggt ttctggttgc ctgttccttg atttctaaga
     1081 ccagtaacca gcaaaagttc cttattcaaa tcccaaaatt cttgatccct tgattccggc
     1141 cttcttcttc tcttcctcga tcacctgttg tcgcagaagc cttctcagga tcttattgga
     1201 gctcgctttg ggcaaggagg cccggaattc gacctgggcg ggtactttat acttggccaa
     1261 gttttttctg caaaagtcga ggatctcatc ctgcgtggcc gaatggcccg tcttgagcac
     1321 aataaaagcc tttactgttt ccccgcggta ccggtctgga attcccactg caatcgcttg
     1381 agcgactttc gggtgttccg ccagcacctg gtcgatatct ttggggtaga tgttgaaccc
     1441 cccggcaatg atcatatctt tctttatgtc caggatgaaa aaatatccgt cttcatccat
     1501 gcgcgcgata tcgccggtat agagccagcc gttgcggatg gtcctggccg tctcctccgg
     1561 acccttccag taccctttca tcacttgggg tccttggatg cagagttctc cgacttctcc
     1621 gggtgaaagc tccttctggc cagcctccag atcgacgatc ttggccaggg tgtctggata
     1681 aggaaggccg atgctcccca gtttgcgctt cccaaagatc ggattgcaat gagtggcggg
     1741 tgaagcttcg gcaagcccat acccctcggt aacccgcgcc ccggtcagtt tttcaaaatc
     1801 ttccagggtg tccacggcta atggacctgc tccgctgtaa catacccgca agctggaaag
     1861 gttatatttc tctgcattct tctcctggga gagggccacg aacatggtgg ggaccccggg
     1921 aaagaaagtg ggccggtagc gcttgatcgc ctgtagcgta cccatgactt cgaagcgagg
     1981 cataatgatc atcgtagcag ccaaatagat gggcacgttc atggccacgg ccatcccgta
     2041 cacatggaag aagggaagga ccgagatgag gacctccttt cctctccgag cgcggactac
     2101 ccagctgctg atctgcaggg tattggccac caggttcgcg tgggtcagga cgactccctt
     2161 gattccttcg ctgatggccc cggtgtactg caggactccc tcatcctccg gctccaggga
     2221 gacaacagga acttttctgg ctgcatattt ttccagcagg ggttccagga gatagattcc
     2281 cttccttttt tggggggagg ggaaaaattg ggacctttcc gatggcttct tacggtccgc
     2341 ccattgccgc aggcaggcga tcaatatatt ctccagcgag ctttgggcgg caatctccgc
     2401 gatcctggga aagagcggtt cggccacaat gagggttcgc accccggaat gcttcagaag
     2461 atgaaggagc tcccgttcca ccgaaagggg attcaggctg acaacgattg cccctatttt
     2521 cagagtggcg taatatccaa tgatgtaggg agggctgttg ggaaggagca ggccgactcg
     2581 atcccctttc cttaccccta atccatagaa ggcgctggcc aaggcctgga cttttccctg
     2641 cgtttctcca taagtgaatt tcttcccggc gaagatcatg gccacactct gggggaagga
     2701 ggcggcggat tgctccagga aatgattcag ggaaaccttg ggatattcca gggagggggg
     2761 aactccgtct tcgtaatact tcaaccaggg agtctggacc atgtcccgcc ttttcgcttt
     2821 cagcgatcag ccatggaccc aggccatgaa agactgaaag ctgaaggatg atcgcttatg
     2881 gctgcctttt aattttttta gctgcttctt ccaccttggc cactagagat gctttgtaac
     2941 ggaccaggga tttcttcaac tcgcggtggt ttccggaaag aatctggacc gccagaattc
     3001 ctgcgttccg cgcccccgcc ttgccaatgg ccacggtggc caccggcact ccaccgggca
     3061 tctgcacggt ggaaagcagg gaatccaggc ccatgaagcc cgattccatg ggcacaccga
     3121 tgaccggcag gatggtatgg gcggcgatca ccccggccaa atgagcagcc ccccccgcgc
     3181 cggcgatgat gacctccacg ccccgccgtt ctgccctccg ggcaaactcc gcagtgcgat
     3241 ccggggaccg gtgggccgag ctgactgtga tttcataagg aacctgaaat tcggtcagga
     3301 ttctcgctgc ttcctccatg accgggaagt cggaatcgct tcccatgagg atggccacca
     3361 gcggcttttg attctccatt ttcgccttac ctttttccca ttcgctgaag cgccttttgc
     3421 ccgatgtctt tccggtaata ggccccttcc caggagatct tggctaccgc ttcataggct
     3481 ctctggatcg cctcctggat tccgcttccc agggcggtca ctcctaagac cctgccgcca
     3541 ttggtaacca cttttccctc ttttagggtc gttcccgcat ggaaaacaaa cgtgtcccgg
     3601 actttagccg cttcctggag gcccgagatg accttccctt tttcatagga gcccgggtag
     3661 ccgccggaag caatgactac acagacggat gcccgatctt cccactctat cttctgctgg
     3721 gcaagggttc catcgatggt tgcttccagc accggcacca aatcgctctt catgcgcatc
     3781 aggaggggtt gggcctccgg gtcgccgagg cgggcgttga actccaacac tttggttttc
     3841 tcctccttga tcatcaatcc agcgtagaga accccttgat acttccttcc ttccttggcc
     3901 attccccgta ccgtgggact catgactttt tccataacct cccggtgcac cctcttattc
     3961 accacgggcg ccggagaata agcccccatt cctccggtgt tcggtccctg gtctttatcg
     4021 taaatggctt tatggtcctg cgaagtcggg aggggcagaa cggcttcgcc gtcggtgaag
     4081 gccagaaagg aagcctcttc tccctccaga aattcttcca ccaccacctt ccccccggca
     4141 gcgccaaagg ctttctgcac catgatgagg tccagggcgg agagcgcctc gtccacgctc
     4201 cggcaaataa tcactccttt gcccgccgct aggccatccg ccttgactac caggggagcc
     4261 cctttgcctt tagcatattt gactgccggt ccatgatccg tgaaggtctg ggaatccccg
     4321 gtggggattc catacttttt catcaggtcc ttggcgaaag ctttgctccc ctcgatctcc
     4381 gcagccttct ggcttgggcc gaaaatcctc atcccctttt ctttaaaggc ctcaacaatt
     4441 cccagagtca agggggcttc tgggccgacc acggtcaggt cgaccctttc cttttccgcc
     4501 cgggcagtca gtcccttcac gtcttcggcg gaaacggata ggcactcggc cagctgagca
     4561 atcccggcgt tccccggagc gcaatagatt ttttttaccc tcgggctctg ggaaatcttc
     4621 cacaccaggg catgctccct tcccccgccg ccgatgacca ggactttcat tttttctccg
     4681 aatcgattca ccacagaggc acagagatca cagagaattt aaataaaaat tctaaacgcc
     4741 aaataaacac cacattcaaa atcaacaata cccaaactgg ctagctgggg atgcttctgg
     4801 attctatttg ggtgtcccta ttggttggga atctagtttt ttttggcggt tcaaatctgt
     4861 tccctgtgtc ctccgtgtct ctgtggtgaa ttcctatttt tttgccaggt actcagaaat
     4921 ggccttgtcg tactttgccg tgagggcata taccttcttc gccagacgaa aattggtctc
     4981 cttgctcacc gcgccgccgt tctttcgcat ctcgttcagt acaggctgat agtccgccgg
     5041 gtccacgatc acggtgacat agggataatt cttggccgag gcgcgcagca tggagggccc
     5101 cccgatgtcg atattctcaa tggcatcttc caaggtgcaa ttcggcttgg cgatggtggc
     5161 ctcgaagggg tagaggttca ccaccaccat atcgatggca aggatcccgt gctccttcat
     5221 cttcttcgca tgttcggggt tgtcccggat tgccaggaga cccccgtgga ccttgggatg
     5281 aagggtcttc acccttccgt cgagcatttc cggaaacccc gtgtactcgg agatgtccag
     5341 gatcttcagc ccgttctccc gcagggtctt ggccgtccct cccgtagaaa ggatctccac
     5401 cccgaaggat tgcacttcct tggcaaaggg gacgatcccg ctcttatccg atagactgat
     5461 gatggccctt tgaatctttc ccatgaggac tcctttctga aaatagcttt cagccatcaa
     5521 ctctcagcgt tcagcagaaa aatgtggcgg agagcttggg gctgatcgct gatcagcttt
     5581 tttaatactc aacggtgatt aggctctttt ctgggggaac gacctctccc acgagagcca
     5641 cttcggttat gcccctttgc cggcatagac ccaggagttt ttccccctcc tcggagggga
     5701 gcgcgatcaa caggcctccg gaggtctgcg gatcaaaaag gatgaccctt tccagctccg
     5761 gcactgcatg ggcaaaggaa atcttcgggc cgaaatgagt ctggttgcaa taggcacccg
     5821 cgggtaccat ccccatggcc gcatactccc tggctccggg cagcaggggg atgagggaat
     5881 aacggagatg gaaggaaagg ccgcttcccc gggccatctc ggaggaatga cccagcaggc
     5941 cgaagccggt aatatccgtg caggcatggg catggcacag ggccatcact tcggccgcct
     6001 tgtcattcag ccgggccatg gagtcgatgg ccttccttaa aaactcctct tccactatct
     6061 ccgccttgtt ggccgtagag atcattccgg ttccgatggg tttggttaag accagaacat
     6121 cccccgcccg agctgcccgg ttggttagta tttttcgggg gtggacggtg ccggtcaccg
     6181 agagaccgta tttcaactca acgtcttcca ccgtatgccc ccccatgagc acggcacccg
     6241 cctcatggac cttttctagc cctccgcgaa ggatctcggt cagaatggat acatccagga
     6301 tagggcgggg aaacccaatc acgttcatcg ccgtcagggg ctttcctccc atggcgtaga
     6361 tatcgctcag ggaattggcc gcagaaatct gcccgaagag aaagggatca tccacgatgg
     6421 gggtgatgaa atccacggtc tgcaccaggg ccatctcttc tgatattttg tagaccccgg
     6481 cgtcatcggc ggtttcggtc cccactaaaa ggttggggtg cctttccagg ggcaggggct
     6541 tgagcgcttt atccaagtcc tccggactca gcttcgccgc tcagccgccg gcctttacca
     6601 tagcggtaag tcgcactttc ttgttcatga tttcttctca aggttcctca ttccgtgatt
     6661 tgggcgatcc ctccccagaa ggccagggcc tctctggaag ccgaaaaatt ttcccattcg
     6721 atcggccgcg gtgaataggc cagccgcaag gcccgtttcc cgccgtcggc cagatactcc
     6781 accagtccct tcaggatgga aaaataaagt aagtcctcgg tggtgtataa agtgaagaga
     6841 aaactcaatt cataaaggtc gcggacttga ttctggcgat ggacatagac cccccacaga
     6901 aacttggatt cgctcgcccc ctgggggttc cgtcggtatt cgtagagact gagaaggggc
     6961 acccagtttt tttcataccc ctcgtaatcc accgggatca agcacggcca gtagagatac
     7021 tcgctcccgt ccgtttcctg gcgccagtag aagaacggcc acacccgcaa ccttcgttcc
     7081 tttttcccct ctttctccca aattttggtt tcatttttgg acaggagcag ataacgatcg
     7141 agtgttcgcc gatagttgtc ccctttatcg tggacatacc aatagatggg ccagaggata
     7201 tacccccttt cgtagctttc ccagtatttt cggccgtaca cggggaaaac gcggaagatt
     7261 tccttctcat cccccttggc ccactggacg aggggccagg ggacatccca ctgggtgtag
     7321 tggtcatcgt catctcgtac gtgcgtaaaa agtggccaga gcaccgaacg cttggcccgg
     7381 cgggatgaag tctcggatac gtagagcggg aacaccatgt tgatctcagt gggatcatcg
     7441 gtgtacaggt accgcttctc gaagtggaag aaaggccaga ggaagaaggt cttctggtaa
     7501 tcgttctcct tccggtcata tcctcccagg ggccagaatt taaacccctc ccttccggtt
     7561 ccttcgctgt aggaaaagaa gggccagagg aaggtgtacg tcttattgtc tccttcccgg
     7621 gaatcggaat agatgggcca gaggaagaag ttcatttcat cccgcccgaa acggtttttc
     7681 aggctcccat aaagggggaa aaaacctcca tagggctccc ctttttccgt ctctccccag
     7741 aaagcgagga gaaatcccct ttcctttttc tccggctttt ctttctccag gtcccggtcg
     7801 gtggaataga tcagccggaa ataggactcc acccgccgat cggtcctttc gtatttgccc
     7861 atggggtaaa gataatccag ccggtattga gccggcttgg tcttcgagta gaacagcggc
     7921 cggaaggcta catctttctc ctccgagtcc ctgcggtaag tgaagaaggg acccagaata
     7981 tcaatccctt ttccctcccg ttcttcatcc tcgctgtaga ggaagagcgg agcgaaattt
     8041 tccctccgtg ggccggggtt cagggtggcg cacccggaaa cccacgaccc caaaagaagg
     8101 atggtcagcc ccgcgatgag atataagaag accctccgtt tccctctgat agccgccccc
     8161 cctaccgaat gaccgtcccc agaagaacgc ccaacacggt gacgggcgtc acttcggtaa
     8221 ttccgtccgc cgccttctgc accgatttga ccgcccgctt ggtatcgtca tagagggcgt
     8281 catccttggc caattttccc agggttccct cccccttctc gatctgttcg gagactttct
     8341 tcaggttggt catggtctct ttggcctcgg tcatggccgc cttggcgtcg ttgaacaggg
     8401 agtcgtccat caccaatttt cccagggttc cctccccctt ctcgatatgg tcggagactt
     8461 tcttcaggct ggccagagcc tccttcgctt cgctcatggt ggccttcgtc tcgttgtaca
     8521 gggcctcgtc cttgaccaac ttgcccagcg tcccctctcc gctctcgatc tgtttggaga
     8581 ccttgttcag gttcgccaag gtctccttgg cttcgatcat cgtcgccttg gtttccacgt
     8641 acaaggcctc gtctttgacc agcttgccca gcgtcccttt tccctcctcg atggattggc
     8701 tcacttccct caggttggcc atggtatctt tcacttccgc gtagagctta tcatccttga
     8761 ccagtttccc cagggttccc tccccccggt tgaccgagtc ggtgatatgc tgcaggttgg
     8821 tcatggtctc tttggctccg gtgaccagct ctttgatcga acgttcccct tcttctcctc
     8881 ccaggacatt actgagcgac cgggtgatcc gctggatatc tccccccacg ctgctgagct
     8941 gggtgagcac ccggtccaca tccgccgaag ggcccccttc ctcgaccaaa ccatccgcct
     9001 tgatgatctc ggtcccggtc cccggaacaa tctcgacgta tttctctccc aggaggcccg
     9061 cggatttcac gtaagccttc gatcccaggg ggagcttcac gttgaaaggc aggagcatgg
     9121 tgaccttggc cttcaagcct tccagcttaa tgccttccac cttgccgact tccactcccg
     9181 caatccgcac gggagagttt ttttccaggc cggccgccga ttcgacgtac gagtaggtcc
     9241 ggtacccttc ttcccggatg gcgatccgcc ccacccggac cgtaaataag gtcaacagga
     9301 cgatccccag gagaacaaag atccccactt tcgtctccgt cgcgatgctt ttcatccctt
     9361 ccctccttcc ggcttttaaa gtttccggcc gatgtccgtg ccccgggtga tcggcccttc
     9421 cacctgcccg tggatgaact gctgaacgat ctcgttcggc gaggccttaa tctccccggg
     9481 agtccccact tcgataatct gccccttgta cagcatggcg atccggtcgg cgatccggta
     9541 cgcactgatc atgtcgtggg tgatggcgat ggaggtgacg tcgagctttt cgcgcatctc
     9601 cacgatgagt tcattgatca cgttggccat gatggggtcc aagccggtgg tgggctcgtc
     9661 atagagcagg atggcgggct ccatggcgat ggccctggcc agagaaaccc gcttccgcat
     9721 tcccccggaa agctcggagg gcatgagatt ttcgatattc ttcagcccca ccagtttcag
     9781 tttttccacg gccgcggcct tgatctcgtc gtccggcagt ttggtatgct ggcgcaggcc
     9841 gaaccccaca ttttcccaga cgttcaggga atcgaaaagg gcgtcccgct ggaagagcat
     9901 gccgaacttt ttccgcagct cgttccattc ctcctcgtcg aggtcggaga tttcggtccc
     9961 atccacgtag atctcccccc gatcatggcg gagcaggccg atgatgcatt tgatgagaac
    10021 gcttttgccc gtcccgcttc ccccgaggac caccatggac tccccgcact tcacctccag
    10081 gttcgccccg cgcagcacgt ggttcgggcc gaaggattta tacaactcct tcaccacgat
    10141 catgggatca tcggggttgc ggcaggattt cttttccccc tctgggtctt cagccatcag
    10201 cgctcagtcc gggcactctg ccctctggat ttcttctccc cattcaaccg acggtactcc
    10261 ttttctccgt cctcgttacg cgggcctgat ttttcgcgaa tccttcctcc cccttttatc
    10321 ctcaaccttg atccctcgct caaatgaaca gggcggtgat gaaatagttg gcgatgagga
    10381 tcagaaggga agacatgacc accgctttgg tggtcgcctt gccgaccccc tcggcccccc
    10441 cctcggtgtg aaatccctga taacatccga tgatggcgat gatcatcccg aaaaccaggg
    10501 atttcaggag cccgatgtag atgtcttcca ggtccagata atcgaaggtg cggcgaagat
    10561 agatggtcgg attgcttccc aagagtttta cgctgaccag gtagcccccc agaattccca
    10621 ccaggtcggc gaagagggtc agaacgggca tgatgatgac cgaggccaga aaccggggga
    10681 caatcaggta tttgatggga ttggtagcca gagtatagag agcgtcgatc tgctcggtca
    10741 cctgcatcgt ccccaactcc gcggccatcg ccgagcccac ccttccggag accattaatc
    10801 cggagagcac agggccgagt tcccgggtca tggacagggc cacgaccgtt ccgacaaaag
    10861 cctcggcgtt gaaacgcttg aaacccgtat agctttgaag agccagcacc atccccgtga
    10921 aagccccggt aatcagaacg acgggtacgg atttaatccc tacttcttcc atctgcttca
    10981 gcaaatttcg aatatcaaaa ggggggcgaa aggtccaggt gagggtctta aaagaaaaga
    11041 tcatcacctt tcccgattcc gccaggaatc tcagaactct gaagccgatg atgtcgaaaa
    11101 ggctgaacat gctggctctt ggaaaaccct tgaagagttt ttctccacta cccttcgctg
    11161 cgctgataga cccgcggtac cctctttccg atgctgcaga ggacctcgta ggggatgctt
    11221 tcgatccatc ccgccatctc cgcggcggtg atctcttcta gtaataccac ttcatctccc
    11281 acctgtacac ccggaatctc gctcacgtca actatggtga ggtccatacg gccaggacct
    11341 ccacttttcc cccggtcaat ccacgcagct gattcagatt gaaccgcaaa gcgtccaggg
    11401 cgatctcggc aatcgacggc cggctcaaat ccgtcctccc ctcacttggt ggtcatggtg
    11461 tacaccccgt cccagtcggc cggcggggga ttcttggaga gggtctccga ccgctccagg
    11521 tatagtttgg ccggcccgtc gtgggggaac tgggctaata ccttctgaaa cgcctcccgg
    11581 gccttgtccc agttcctctt ccgatattcg gccagagcgg cgtggaagcc atcggccaga
    11641 gcccgctggt cggtggatgc ggtctttacg agagagagaa gctcgaagat cttcaccggc
    11701 tgtcccttcc ctttcacccg caccaggtcc agttccctcg ctataaattc ctcctggacc
    11761 ttctcaaggg tcgtctggct cacgatgatg ttggtcccgt acaccttgtt taacccttcc
    11821 agcctcgatc cggtgttcac gctgtctccc atcaccgtgt agttgaaccg ccggtccgac
    11881 cccatgttcc cgaccaccat gggcccggcg ttgacgccga tcccgatatt caggacgggc
    11941 aacccctcgg cgctccattt cttctgcagg cgatggagtt cgtcgagcat atccagggcc
    12001 gtcagacagg cgcgcctggc atggtcgggt tgatccaggg gcgcccccca cacggccatc
    12061 accgcgtccc cgatgtactt gtccaggagg ccgtcatatt tgaagacgac gtccgtcatc
    12121 ttggttaggt actcgttcag gaacttgacc agcccatccg gagtcatccg ttcggatatc
    12181 gaggtgaacc cccggatgtc gctgaagagg acggtcaggt ctttcttttc gccacccaat
    12241 ttgagtttgt ccggattccg cagcatctct tccaccaccg aagcggtgag gtaatattgg
    12301 aaggctcccc ggatcctctt tttctccttc tcctccgtca tgtaccggta cccggtaacc
    12361 cccaggtaga tgaaaaccag gttgagggat ggatacgtga gattcatcca gactccctgg
    12421 gtctcaaaaa gatattggcc cagggctagg accgaaagga ataaagcccc ccccaccagg
    12481 gctccccagt gggccttgaa ccgcgggaga aggcccccga gaatcagccc cacaaccgcg
    12541 ataaccagga tgtccaccag ggtggcccag tttggccggt agagaaactg gttttggaga
    12601 atggaatcga tgacgttggc gtggatctcc agcccgggat atacatgttc gaaaggggtc
    12661 acccgcatgt catagatgcc gatggccgtt gcccccacca aggcaatttt tccctggaag
    12721 gctttttccg ggacccgtcc gtgaattaca tcggtggccg agtaatgagg gaaagtcttt
    12781 tgcgggcccc ggtagttgat gagcagccta ccctcttcat tggctggaat cgacaccttt
    12841 cccaggcgga cctggtccac cccgtactcg gcgatgcgca gcgccagagg ggaattatcc
    12901 tgaaatttct gcagcaccgc cagggagagg gaggagtaga atcggtcctg atacttaatc
    12961 accagagggg cccaccgcac cgtcccgtcc gggtcgggga atatgttgaa gtaccccgct
    13021 ccttcggcgg cctgggaaat gactggaatg tttacctcgg cgtgaaccgc ttcgaagacg
    13081 ggaacccggg ccgcctcctc ggaggtgaac ttgactaggt tgaaagaagt gaggggcggc
    13141 atttctttcg ccggaccttc ctgcccgcca gaactctccc ggggggaaaa atggaagaag
    13201 tagccgagaa cagcccgttg ggagcgggct agggaatcgg ccaaaatccg gtcggagtca
    13261 gcctcctgaa gcgcttcggc aatgtagcgt tccaggttcg ggttggccac tttcaattcg
    13321 gtaagcttct gtttaatttc ggtcagaacc ttgagctggg aattttcatc cggttccgcc
    13381 cagacgatat cgaaccccac aaccttgacc tccgctttca ccaaagcatc cagaacctgg
    13441 gccatgcgga ctctcgtcca gggccagcgc ccaagttcgt caagggattt ttcatcgatg
    13501 gttaccagaa ccacctggtt gctggtggga atcttacccc gggaaaggaa gcgtgcgtcg
    13561 agggttttta gttccagaat gctcaagaaa taggggtcca ggtggtaaat ccccaagacc
    13621 gcgagggtca gcaggagggc gatcttaaat cctgaaaggt taaagaaatt cttcagattc
    13681 aggcctttct taagggtttt tgcattagaa gtttaacaaa tccaatccca aaaaacaatt
    13741 ccccagatgg tgcgagaccc aagcaaccgc tcaggaatcc gggtttttcc cgctgatttt
    13801 gtcccgctat ggcagaacac acccccttgg ctgagaatat ttctgttacc cttccatcca
    13861 ccggatcagg agaagagctg aatttgcttt ctcccgccat tgacggggtc ggaatttggt
    13921 ccttggaatt ttgtcttttt ttattggtgg gacttgaaaa gtccaccctg cctgtggcaa
    13981 aattattttt atggaatcga tggggaaaag cacggcgacg gccgacaatc ggccaacctg
    14041 tttcggcgac gaggccaagt tcgtagctta tatggatgat gccggggaca actccgagtg
    14101 tggccgttgc cccagcgaaa atgactgcgg ggaatttatc ctccttaagt gttcccggga
    14161 attgatcttt taatcgaata aataggaggt aaccatttcc accgaggaca accgggagaa
    14221 agcgattcca tcgaagtaca taaaatcgat ttccaaaccg cttcttatgc tgcctattta
    14281 gagagccggc gtagctcagt ggtagagcag ccgattcgta atcggctggt cgggagttca
    14341 actctccccg tcggctccag gctatctttc tgttcatctc ccaatcattc gacctcaggg
    14401 ccattttggg acggtcatcg ttaccttgta gtggcggccc tcgcaaaatt tcgcttaggc
    14461 ccagcaatgc cggggtagga aataaacggt aaagagtttt gaattttaga aaaattttat
    14521 gtagtctgag ctaattcatg ggggaaatag ctctttaaat agaatataga ttccaataac
    14581 ttccccttaa aagatgatct cagtcagaaa aatttacgat ttatcaactt ttcagatatt
    14641 tttagaattg acaaaaaaat aacaatatta tatacttaca ataagctttc tgtggggaat
    14701 catatttgct ctgttcgact gatggaaaat cttgccttaa aaaaggcggg caaggttttc
    14761 ataattgggt gaatgccctt ggaagggcat cttgccttgt tccttcctct ggagacaaca
    14821 catttcgtga gggatttttg ggaaaagagg gtgcctcaaa gtaccttgca aaaagccttc
    14881 gggtggtaat tctcctttgg atggcttctt tggggaactg ccaggtttgg atgaaagata
    14941 tttccatcct ttcaagaggc tgggaatcgt tggttgccga atgccataaa tgaacaagga
    15001 gggttcccat ggaagaggac aaaagcgggg ttgataagtt catgaacatt gacaaattca
    15061 ttactgagct aatcgggtat catgagcaga tcaagcaatt gaaggcctcg gaagtccact
    15121 acaaggacct ggttgggacc cttcaagata gcctgaacaa ataccgggcc gtcatcgacc
    15181 atcttcctca aaaggttttt ttgaaggata aaaatctatt ctaccttttt ggcaaccaaa
    15241 attatgcccg cttcttggga gtggacccac aaaaaattcc cggaaaaacg gatcacgact
    15301 ttttccccta tgaaaaggcg gagcaatacc tgaatgatga ccggaagttc atggagcaag
    15361 ggcatccgga agagaaagaa gaaagggcta cccgtgacgg aaaagagcgg gtggagcagg
    15421 tcgctaaata tccggtcagg aatgaggccg gagaggttgt gggaatttta gggatatcct
    15481 gggatatcac cgataaaaag gaaagagagg aggacttgga gagaaggtcc ttggaactca
    15541 cccgacttct ggaggtccgc acggaagaat ggaaagaaat cggtggaaaa tttcaatcgg
    15601 agcaggccga acgccggcgg ctggaggaaa gactcaaaag cttggaaggc tattatcgga
    15661 ttctcttcga gaataccggc acggcggtcg cggtggtcga agacaacaag gtgctatcca
    15721 gagtaaacac ggagtttgaa agattttcag ggtattctcg ggcagaagtc gaaggagcga
    15781 aaaattggaa cgaattcatt cataatgggt ctccgcaaga tatggggaaa gcaacgggtt
    15841 ctccagatat gagttccccg gacccgggaa tgcaggtttt caagttcgtg gataaacaaa
    15901 acagggaaag aaccgtttcc atgaccgcag cccggattcc agacaccaat cgagtcatgg
    15961 tatccctgat ggatattact aaatacaagc aggcccggga ggaattgaac cggatcatga
    16021 agcagttcat ggaattgatg gttgaaatgg aaaggggagt aaagaacctg gatggatagc
    16081 cggcttatcg ggtgaccgaa actggaacca aaggatttgc aatccggagg tctgtaaacg
    16141 gaatcatccc gagaatcgcg tcgaagttgg atttcatgaa aagcggatat ccataaaaaa
    16201 atgaatttct tccgaaaaaa gaaaaaatta ttgaacccct ttctatgtga cccgaaactg
    16261 aaattcgtgt tgcaggaaaa tacgtacgag agcttctata gttttctaaa agaccccttt
    16321 gcctctcatc ccgaccccaa cctctttttt ttgacggaca actgccgaga agtatggaat
    16381 tcggtgcttc agggcatttt cgaaagaaag ggattcatct tactaaccgg agaaaatggg
    16441 atcggaaaaa caaccctaat ggccctggtt tatttatttt taaccaccaa tggcgggaaa
    16501 gtgaaggtga tccccctttt tgaccctccg aatgccatcg aggaaattct ccgggcggtc
    16561 ctccgaaatc tgggatttcc ggcagaagaa gagaataaga gtgccatgct ttccctgctg
    16621 gatgaggata tcctccaaag gtctgcccag ggggaaaccg tggccatgat cttcgatgaa
    16681 gctcagaacc ttcgaaaaga agtcctggaa gaaatccgcc tgttcgccag cccccacccg
    16741 agaagaccca agtttctcca ggaaattttt gtgggggccc cgcaatttga aaagaaactg
    16801 aatggcaggg atttattggt tctcaaccag agatttgagg tccggtgccg cctccgcccc
    16861 tttaccctgg aggaatccct gggttacatc gagcaccggt tgaatagagt gggaagcacc
    16921 acgtccaagg tcttcactcc aagggccgtg tacctgatcg cctgtcacgc caaggggatc
    16981 cccgggaagc tcaaccgggt ctgtcaggag acgctctcag tcgcctatac ccagatgaag
    17041 gagaaagtcg attcggccaa tgtaagagag gcccttgcaa atctcggaat ggttagaaag
    17101 gaattatggc aactccggga gaaaacccta tcctggttta aaaggaattc ggcaagttta
    17161 taatgatcat ggctgtccaa aattttgata gggaagggct ttcggtcggg tttcgattgg
    17221 caatccgccg cccgggagtt caattctccc cgtcgactcc cccgcttctt tccccatagt
    17281 cgttctcctg cggcgtttgt gcccctcatt ttgacgacag tcttagccaa catcttgggt
    17341 cgatgaaccc cgccgccgga ttgggttcag ttcgtgctct gtgcctcggt ggaaagggtt
    17401 ttcggattcc tggaaaagac cagttttttt gatttaagaa agggtttgag ttcttaaatc
    17461 gccgtattta agcccataat ccccgtataa gggtttggga attcaatcag ttcgtaatcc
    17521 cattctgccc aatcaccgcg tgccgctgcc ctatgccccc aagcctattc tgcatcgctg
    17581 aatcatgaag ttggggtccc accgcggagg agcttaattg gtgcgggcca agattgtatg
    17641 ggcgatatcc gttcgttctc ctccgcgcag catatcgttc cggggcgatg gcgaggccat
    17701 atacccagat ctggcttaat ttttgcttcc caagggggag tattttcgtt gaattcatta
    17761 aaaaatttcc aaaatactga cgataataga aaatagcccc atggaatttg aggtttttaa
    17821 tctataatta gaattttctg gggatatcga agaagatgaa ttttggaaaa actaccccgc
    17881 aaggcgcgga taattttgaa tttatttttg aaaacgcgcc ccagggcatt tatctattaa
    17941 cctcggactg gaaattcctc accgtaaatc cagccatggc ccacattttg gggtatgaat
    18001 ccccgagcga tctgattgcc agcttcaccg acccgcatcg tcaatattat gtctcctctg
    18061 agcgaaatgc aaaattcaga gaattggtta ctgtccaggg gatggtccgg gattttgaag
    18121 cccaggtttt tcggaaggat ggaagcatca tttggatttc aaccagcgcc cggcggatac
    18181 aggaccccga agggaaattg ctttacgttg aaggcttggc cacggatata accgaggcca
    18241 agcaattcga agaatcctca aaggacttgg aagaacaaaa tagacttctg attcagcatt
    18301 ccaatgacgc catttttatt gcccaagatg aagtgataaa attcgcaaac cctaaggccg
    18361 aagaattatt cggctactca gcggcggaga tggccaaaat tccatttggg aatcacattc
    18421 atccggatga tagggagatg gttctcgaaa agcaccggag aaggttagaa gggcagccca
    18481 ttcccgctat ctcccccttc cgaatcatca accggtcggg tcaggaatta tgcatagaaa
    18541 caaatgcggt ggttatcgaa tgggggaaaa agccggctac tctgatgatc gccagaaacc
    18601 tgagcgaaga gaggaaagaa gaggttgagc ttgggaaggc tcataatatg taccgggcga
    18661 ttctgggaaa tatcaatgat atcgttttaa gtttggatga taaaggctgt ttaacctata
    18721 tcaacccggt ggttgaaaag aaactatcct ataagatgga agaaatgacc gggaagccat
    18781 tatcctcctt cattcatcct catgccgaac ccgattttcg ggaaaatatg aagcgttgcc
    18841 tttcgggcgc ccaaagaacc tttgaattcc gcattctgga caagaagaat caaattttcg
    18901 atatgcgctt ctccggccgg ccgctggtca aagatggaca aaccatcggt ctaaccggga
    18961 ttttgatgga tataaccgaa caggagttat ccgcgacact cctcaatagg gcggagagaa
    19021 attaccgcag catttttgag aatgccttgg aggggatttt ccaaagcacc atcgatggcc
    19081 attttctggt ggccaacccg gcttgcgccc ggatattagg ctactcgtcc ccggaagaat
    19141 tgattacccg ccatttatcc ccggatcgcc ccttctacgc caatcctgaa attcggaaag
    19201 aatttcaaac tcttttacag gaagtcggag tggtcaaagg gtttgaatat gaagtctacc
    19261 ggaaagatgg ggaaaagatt tgggtttcgg aaaacgccct ggccatgcgg gattccaatg
    19321 ggaatgttct cttctatgaa gggaccctcc aggacataac cgataagaaa caggctgagg
    19381 acaagataag atttctgtcg ttctatgaca gtctaaccgg ggtttataat cgggcttttt
    19441 ttgaagaaaa tttaaggcgt ttggattctc ccagacaatt gcctatcagt atcctgatgg
    19501 gagatgccaa tggcttgaag ttgatcaacg atgcctttgg tcatcaagag ggcgaccggt
    19561 tcctacaaga gattgccgcc attctgaaga gttgctgccg aaaagaggat atggtggccc
    19621 gtttgggagg cgatgaattt gccattatgc tgccccgaac caaccatggg gtgtccatgg
    19681 aaatcaccga gaggatcaag aaaatctgca aggaaaaaag cagtgctcct tttgaggtca
    19741 gtatggcatt gggagtggcc accaaagttg acccttcgaa gagtatcgaa gtagtcctca
    19801 aggaagccga ggaccggatg taccggaata aactgctgga aagcaagagc tttcggacct
    19861 cccttctctc ttcgctccgc cagactcttt ttgaaaaaag tcatgaaacc gaagagcata
    19921 cccggcgcct gcaacggttg gcccttcaaa tcgggaacgc tttaaagctg cccgacagta
    19981 ttctggatga attgaccctc ttggccaatt tgcatgatat cggcaaaatc gccatcccgg
    20041 aagaaattat catcaaaccc gacaggctgt ccttggagga atgggacctc atcagaaagc
    20101 atccggaaat cgggtaccgg atcgcaaact cctctcccga gatggttcct gttgccgaag
    20161 ggatattatc ccatcatgag tggtggaatg gagccggcta tcctcggatg ctgtttggaa
    20221 aagaaattcc cctggcctcc cgaatcattt cggtggtcga tgcctatgac gttatgattt
    20281 acggtcgccc ctacaaaaag gccttgagta aacaggaggc tctcgcagaa ataaagagat
    20341 gtgccgggag tcagttcgat cctttgattt ctcaggtttt ccttaagatc ctttctggcg
    20401 ccaagctggg tgaaatcata gccagccaac tcatcccgga tgtcttataa tccttcccga
    20461 agattagggg aggatgacga tagaaaatct gcctctttcc acgtagataa attccagacc
    20521 ttgtagaaga tactcttttc ccccccgtga cgaacatcaa tggttaggtc aggcacccct
    20581 catccagccg atgggcctac cttacctttg ccccgacctt ggcctccttg tcgaaagtgg
    20641 ccaggacaac accggtgtcg tcggccccgt cagcagccag gagcatgccc tgggactcga
    20701 cacccatgag cttggtgggc tgaaggttgg ccacaatgat gatggtcttt cccaccagtt
    20761 cctcgggttt gtaggctttg gcgattcccg ccactagggt ccgctcctcg ccgatgtcga
    20821 tgcgcagctt caggagttta tcggatttct tgatattctc ggcggcgagg accttcgccg
    20881 cgcgcaaatc caatttctgg aattcttcat aggtgataat gttcgaggtt tccattttct
    20941 ctttttcctc tcttttgggc gcttcttcta tccgcgggaa cagagattct cctttacgca
    21001 cctgcccacc cggtttcaac ccgccccatt cctccaggct ggccagattc tggcggtccg
    21061 gctcggagat tcccaggtag gcctgaatct tttcggccgt ctgaggcata aaaggcgaaa
    21121 tgagaacggc cgagaatcgg agcacctcaa ggagctggta gagaaccgtg tcgaggcgct
    21181 ccgctttttg agggtcttta gccaaagccc agggggccat cccgtcgata tatttgttgg
    21241 ccaggttgat aagctcccag gtggagatca gaagtttatg aaaaccgagc tgttccatcg
    21301 tgggctcggc ttcccgggca atcctcatag ccaggtcttt gagctctatg tccttatcct
    21361 cgagtccggc cggccgggga atgactcccc cccggaaacg ctccgccatg gcaagggtcc
    21421 ggctgaccag attccccagg tcattggcca ggtcggcgtt gattcgtccc accagggccg
    21481 tttcgctgaa gttggaatcc aggccgaagg ccatctctct caagaggaag taccggaaag
    21541 cgtccaggcc atatttgtcc ttgaggtcca gcggttttac gatgttcccc cggctcttgg
    21601 acattttgga atcggagacg atccagtacc cgtgcacgtt tagatgctga tactggggga
    21661 ccccggctga cttgagcatc gtgggccagt agatgccgtg cggcttgagg atatccttgg
    21721 cgatcaggtg ctggcagacg ggccaaaggt gcccgtatcg ctctccgtcg gggtaatcaa
    21781 cccccgtgag ataattgatc agggcgtcaa accacacata ggtgacataa ttggaatcga
    21841 agggcagggg aatcccccac tccaggcggc ttttgggccg agagatgcag agatcctcca
    21901 gggggtcccg caggaagccc agcacctcgt tgcggtaccg ctccgggcgg atgaaatccg
    21961 ggttagcctg gatgtgttcg atcagccacc cctggtattt gctcatgcgg aagaagtagt
    22021 tcttttcctg gataaaatcc ggagccgtcc cgtggtccgg gcatttccct tcgatcagtt
    22081 ccttttccgt gagaaaccgc tcgcatcccc ggcagtaatg cccgccgtat tcgccgaaat
    22141 agatgtcgcc ggcatcatac accttctgga ggatgtgctg gacaaccttc ttgtgggctt
    22201 cttcggtcgt gcggatgaag cggtcatggg tgatattcag cttgggccaa agtgcccgga
    22261 agaggccgct gatgcggtcg gcatacgctt gaggggtttc gcccgcggcc cgggcggcct
    22321 gggccacctt gtcgccatgc tcatccgttc cggtcagaaa gaaggtatcg tatccggcca
    22381 gcttataaaa acgattcaac acgtcggcca cgatggttgt gtaagcgtgc ccgatatgcg
    22441 gctccgcatt tacgtaatag atgggggtgg taaaataaat tcgtttcatt ttcccttcct
    22501 gacccggtca aagaatgatt cctcccggac ctcatcgaaa cccacttcca cttctttccc
    22561 gtcatccagg gtcactttca gtttctggtt gatgatgctt tgctgcatga ccttccccgg
    22621 tccctggggg gtgatcactc ttttacccac cttgggcagg tctttcttca gctcggagta
    22681 agtgtccact tcataggcca gacagcacat cagccggccg caggccccgg agattttctg
    22741 agggttcaaa gccaggttct gctctttggc catcttcacc gaaaccggct cgaattcctt
    22801 caaaaaggcg caacagcaga actcccggcc gcaggttccc agcccgccga tcatcttggc
    22861 ctcgttgcga acccccacct ggcgcatctc gatacgggtt ttgaaagcac tggccaggtc
    22921 tttgaccagg gcccggaaat caaccctgcc ctcggaaaca aaatagaaga tgatcttact
    22981 cccatccagg aaggattcga ctccgatgag cttcatgggc agcttgtgct cctggatctt
    23041 ggccagacag acctttttgg cctccctctc ccggatcaag ttgtgacggc cctgttccat
    23101 atcctgctgg tcggccaggc ggaggacctt tttcaaggtc ttggtgaaat atttggggtc
    23161 tcgttccagg ggcatccggg ccacaaagcc gatctccggc ccccgttccg tatccactac
    23221 cacgatgtcc cgttccttca gagtgaatcc cgtggaatcg tagtcgtaga ttctcccgtg
    23281 gtcccgaaat tttactccca cgactttcat aaagaatgcc taaaaggcac tagagtgcct
    23341 aaaatgccta aaacgaaata cgtttcgggg ttggagtttc acgtcttttt tgctcacact
    23401 caaacgggca ctgacccttt caaccctatc cttgttcatc ctctttccat cggtctctct
    23461 tttcttattt ccagcatcaa ggtttccaag gccaactgct tgttcacgtt gtaggccagg
    23521 gatctttgga cctcggagat caggctgaat atgccttcca gccgatcgaa ggtggacttg
    23581 gctgcgtctt tttccacagt agagagaagg tcgtggttga tcagaccttc tttggttcga
    23641 tccccctgca ccttgaagac ggtcagatcg cgaacccaga ttttccatag gtccataagg
    23701 tctccgatct cctcttcctc cttggccagc cgttcgcagg tctcgaagac ctcttccgtt
    23761 gatttctggg gaagatcgga gaagacctgg agccaatcca gacgtttctg gaagtcgacc
    23821 cgggcactca gggacaccgc ctgtcctgca ctccccccag ccagagatgc gtaaaaacgg
    23881 gctttttctt cgtccaaacc cttgtctttc tttagaatct gagcaatgtc tggctgggat
    23941 aagggcttaa acttgaccca ctggcagcgg gagaggatcg tggacaggag ctgatggggc
    24001 ctggcggtta tgagaagcag gtgagtatcc cggggcggtt cttccagaat tttcagcagg
    24061 gcattcgagg cttcggcttt catctgatct gcgggatcga tgatgcaggc ccggcgtccc
    24121 ccctccatgg gccggaagcg cagccttttc tgtatttccc tgacctggtc aattttgatg
    24181 aattctccct cgggctccac ggcgatcaca tcggcatgat tcgaagagtt gaccttgatg
    24241 caggaggtac atttttcgca gcaatccccc gcctccggag gagactgaca gttcatggct
    24301 ttggctagag tcaaggccgt taggtgtttg cccacccctt cagggccgat gaaaaggtag
    24361 gcatgggcca gccgatcatt gcccagggca ttgcgcagaa tggctatggc cttttcctgt
    24421 cctctgatct ggtcaaatga cacgtttcat cctcgtaggg aaccggggag atgagagacg
    24481 atctcccgat gaagagccgc ttcgtctttc tttccgtcca ggacgatgat ccgaccgggt
    24541 tcttttcggg ccagggttaa ataaccctct cttatccgct gatgaaaatc aagagcttcc
    24601 ttttcaaacc ggtcctcttg ggtattgtca ttctccagcc ttttccaggc ccttctcagc
    24661 ccaacctcca ccggcaggtc taaaaggaaa gtcaggtccg gttttatatt ctccaaagcc
    24721 cgggcatgga tctcttcgat ccaggccaaa tccagaccac gcccaaatcc ctggtaaact
    24781 gtagtggcat cagcaaaccg gtcgcacagg acggccttgc cttcctggag ggcaggagca
    24841 ataacctgat ggtaatgttg aatccggtca gcgatataga ggaaaagttc ggtcccgggc
    24901 tccatgcccg cattatctga agaaaggaga atctgccgga tcttttctcc ta
//
LOCUS       DASPRR010000073         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_503676, whole genome shotgun sequence.
ACCESSION   DASPRR010000073 DASPRR010000000
VERSION     DASPRR010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503676"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VEL68_02640"
     CDS             complement(<1..234)
                     /locus_tag="VEL68_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34897.1"
                     /translation="MKNRSTLLLALFLSSLMLKILIFLVVTEPILFSKYPIFAERISQ
                     GVDLGERILDLSPLYLYLNLLFQKVYGRSWEGLA"
     gene            632..1402
                     /locus_tag="VEL68_02645"
     CDS             632..1402
                     /locus_tag="VEL68_02645"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXZ34898.1"
                     /translation="MVEGFTLLFVFLLGCCVGSFLNVCIYRLPQDLSIIAPRSYCPQC
                     RAPIHGYDNIPLVSYLLLRGKCRNCGAKISWRYPLVEALSGGFAVALFVKYGFGFFSY
                     SAFTAALLVITFIDLDHRIIPDLISLPGIGIGFLLSVFEPSLSVRDSLIGLVAGGGSL
                     YLVAVAYEALTKREGMGGGDVKLLAMIGAWLGWKAVLFTIFFASLSGTIIGGGAMLAQ
                     KQGRHSAIPFGPFLAFGALAYLFFGPQLIDWYLNLGKP"
     gene            1550..2113
                     /locus_tag="VEL68_02650"
     CDS             1550..2113
                     /locus_tag="VEL68_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXZ34899.1"
                     /translation="MKQRSTRFRRLFTPTLPSPIEGEGESTLKNLKGQAENRSLYQPT
                     VSAGFLKSEPNEHESSGKAHSITQRIIGSAITVHKELGPGLLESSYEACMAYELARLN
                     LKIERQKGLSVTYRGIKLDCGYRIDFLIEDRVILELKAVEKLEPIHEAQILSYLKLSK
                     CEVGLLINFNVRLIKNGIRRFINKVLE"
     gene            2157..3815
                     /locus_tag="VEL68_02655"
     CDS             2157..3815
                     /locus_tag="VEL68_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ34900.1"
                     /translation="MTWSFRPGIRTQIVFTLTILMAGAVAFVGFLFLKVEERNLMDQK
                     IREGKQIVGALQRFLQDWAPESPGKTLSENLDRVVFLFAQGHPDLHFTVVNRDFNILA
                     DSRPDQVGKVLRDMGLEKAMASGKIFAHGAESEESFSLMKRTPLLISSAWLLGDQTVG
                     GIRAEIPLEDLKETLFRSQSILFLYFFLTAFLLIVVGSFLLSRVILKPLKKLMQMSDK
                     IAEGNLELMSGPSGGNEVGRVLASFNHMISRLREDRSKMEEYIASLKKVNRELRLAQE
                     EIIRSEKLASIGRLAAGVAHEVGNPTGAILGYLDLLSKGGLAEAEKAEVLKRAELEAE
                     RIRRIVRELLEFSRPSARREEEVEVNTVIDRALSLLSHQKKVWEQIHVIRELQKDLPR
                     WRGNPHHLQQVMINLLLNAADALVANDSGQTGGEKKIRITTQALPPEELGDSLGPAAR
                     RKEVSPGADSSLFGIDRDVHPPSLQEVSSILQVDVEDSGPGIPAERLGRIFDPFYSTK
                     PPGEGTGLGLAICLGILESYGGKISVQSESGKGARFTILLPIFE"
     gene            3901..>4305
                     /locus_tag="VEL68_02660"
     CDS             3901..>4305
                     /locus_tag="VEL68_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ34901.1"
                     /translation="MENKKALVVDDEEAMRHMLSLVLKREGYEVLTAADGKEALSKLE
                     SDLFDFILMDVVMPEMSGLELLEAMKDRKVESTIIMMSAYGSLDTAVEAMKRGAYDYI
                     TKPFKPDEILLALRKAEERETLRKENAQLRQVV"
BASE COUNT         1026 a         1060 c         1214 g         1005 t
ORIGIN      
        1 ggccagacct tcccagcttc ttccgtagac cttctgaaaa agcaggttga gatagagata
       61 gagggggctc aggtcgagga tgcgttcccc gagatcgacg ccttggctta tccgttcggc
      121 gaaaatggga tacttgctga agaggatggg ctcggtaacc accaaaaaaa tcagaatttt
      181 cagcatcagg gaggagagaa aaagggcaag cagaagagtg gatcggttct tcataggatc
      241 gcagatttgg gttcagatac ccttccaggg ggccaattac ttcattttaa ccgatgaagg
      301 gttctttggg gaaggttttg gccagcaccc cgggatatgg attgaagcaa tagaaaagat
      361 atgtctgaga atctcatcgg caggggtcct tccttcctaa acccgtgagg taaaggggga
      421 gatgccaggt tcagaaaata aaacccattt tccccggagg cagatgtccc gggggggaaa
      481 ggtaatcgaa ttccggttaa gaattgacac cttctaaggt cccatactat attcagaagg
      541 gagggtttaa aagtgaagga acatccgttc cctaatagaa tcaccccgaa ctggactgaa
      601 ataaagattc cgagagggaa accggaggac catggttgag ggattcaccc ttctttttgt
      661 ttttctcctg ggatgctgtg tagggagctt tctcaatgta tgcatctacc gcttgcccca
      721 ggacctttcc attatcgcac cccgctccta ctgcccccaa tgccgggccc ccattcatgg
      781 gtatgataat atccctctgg tgagttatct tctcctccgg ggaaaatgcc ggaattgcgg
      841 ggccaagatc tcttggcgat atcctttggt cgaagcgctg tctggtggtt tcgccgtagc
      901 tctctttgtg aagtacggct ttggcttctt ctcctactcg gcattcaccg cggcgctcct
      961 ggtgatcacc tttatcgacc tggaccatcg catcatcccc gatttgattt cccttccggg
     1021 gatcgggatc ggcttcctcc tttccgtctt cgaaccctca ctctcggtga gggattccct
     1081 gatcgggcta gtggccggag ggggctcgct ctacttggtg gcggtggcct acgaagccct
     1141 taccaaaagg gagggcatgg gaggcggaga cgtaaagctg ctggccatga tcggggcgtg
     1201 gctgggctgg aaagcggtcc tcttcaccat cttttttgca tccctttcgg gtacgattat
     1261 cggaggaggg gctatgctgg cgcagaaaca gggaaggcac tccgccatcc ccttcggccc
     1321 cttcttggct ttcggggcgc tggcttatct cttcttcggg ccgcagctca tcgactggta
     1381 cctgaatttg ggaaaaccgt aaagaaatct taaccacaga gatcgcagag accctagaga
     1441 tgaatcaaaa tgtaaattca ataatgggct tttccattta actcatgctg acatttccag
     1501 cccgaacccc ccctctcccc ttcgtgggag agggtcaggg tgagggggat tgaagcagag
     1561 aagcacgaga ttccgaagac ttttcacccc cactcttccc tcccccatcg agggggaggg
     1621 agaatcaacg ctgaagaacc tcaagggtca agcggaaaat cgttctttgt atcagccaac
     1681 cgtatccgcc ggatttttga aaagtgagcc gaatgaacat gagtcaagtg gaaaagccca
     1741 ttcaataact caaagaatta taggatcagc gataacggtt cacaaagagt tgggccctgg
     1801 cttgctggag tcttcttatg aagcctgcat ggcctatgaa ttggctcgac tgaatttgaa
     1861 gattgagaga caaaagggat tgtcggtcac ttatcgtgga atcaaattgg attgtggata
     1921 tcgcatagat tttcttatag aagaccgggt gatccttgaa ctgaaggcag ttgaaaagct
     1981 ggagcccatc catgaagcgc agatattatc ctatctcaag ctatccaaat gcgaggtggg
     2041 acttctgatt aactttaatg ttaggcttat caaaaatgga atccgtcgat tcataaacaa
     2101 ggttttagaa taatttatct ctctgttctc tgggctctct gtggtgaaat ttctttatga
     2161 cctggagttt tcgacccggg atccggacgc aaatcgtatt tactctcacc attttgatgg
     2221 ccggggcagt ggcctttgtg ggattccttt tcctgaaagt ggaggaaagg aatcttatgg
     2281 atcagaaaat ccgggaagga aagcagatcg tgggggccct gcaaaggttt ctccaggatt
     2341 gggccccgga aagccctggg aagacccttt cggagaatct tgaccgagtt gtcttccttt
     2401 ttgcccaggg ccaccccgat ttgcatttca cggtggtcaa ccgggatttt aacattctgg
     2461 cagactcacg gccggaccag gtaggaaagg tcctgcggga tatgggcctg gagaaggcca
     2521 tggcttccgg aaaaattttt gcccacggag ccgagtcgga agaatccttt tcccttatga
     2581 agagaacccc ccttctgatc tccagtgcct ggctcctcgg ggatcaaacg gtgggcggaa
     2641 tccgggcgga aattcccctg gaggacctga aggaaacgct cttccgctcc caaagcatcc
     2701 tcttcctgta tttctttcta actgcctttc ttctgatcgt ggtgggaagc tttctcctat
     2761 cccgggtgat cctaaagccc ctgaagaagc ttatgcaaat gtcggataag atcgccgaag
     2821 ggaacctgga attgatgagc gggccttcgg gcgggaatga ggtggggagg gtgttggcct
     2881 cttttaacca tatgatttcc cggctgcgag aagaccggtc caagatggag gaatatatcg
     2941 cctccctgaa aaaggtcaac cgggaactgc ggctggccca agaagagatc atccgttccg
     3001 aaaagctggc ttccatcggg cggctggccg caggcgtggc ccatgaggtg ggcaatccca
     3061 cgggcgccat cctgggttac ctggacctcc tttccaaagg aggattggct gaagcggaga
     3121 aggcagaggt cctgaaaagg gcggagctag aggccgagag aatccgacgg atcgtgcggg
     3181 agcttctaga attttccagg ccctctgctc ggagggaaga agaggtggaa gtgaatacgg
     3241 ttatcgaccg agccctttcc ctcctttccc atcagaaaaa ggtgtgggag cagattcatg
     3301 tgatccgtga acttcagaag gatctccccc ggtggagagg aaatcctcat cacctgcagc
     3361 aggtgatgat caacctcttg ctgaacgcgg ccgatgcctt ggtcgctaat gactcgggac
     3421 aaaccggggg ggagaaaaaa atccgaatca ccacccaggc cctgccccca gaggaactgg
     3481 gtgattcttt gggtccggcc gcccggagga aagaagtcag cccgggggcg gattcctctc
     3541 tttttggtat cgatcgagat gttcacccgc catccctgca ggaggtgagt tccatcctgc
     3601 aagtggatgt ggaggatagc ggcccgggaa ttcccgcaga aaggctggga agaatcttcg
     3661 atcccttcta ttccaccaaa ccgcccggcg agggaactgg cttgggtttg gccatctgcc
     3721 tggggatcct agaatcgtac ggtggaaaaa tctcggttca aagcgaatcg gggaaggggg
     3781 cccgatttac cattctttta cccatttttg agtagcctct tggacccccg acgacagaga
     3841 aagaaaaggg cggccctgcc tcgctcggcg ctctgcagtg aaacctttta ggggaaagac
     3901 atggaaaaca aaaaggccct ggtggtcgat gacgaagaag ccatgcgtca catgctctcc
     3961 ctggtcctaa agcgggaggg ctatgaggtc ctgaccgcgg cggatgggaa agaggccctc
     4021 tccaaactgg aatccgatct ctttgatttt atcctgatgg acgtggtcat gcccgaaatg
     4081 agcgggctgg agctgctcga ggccatgaag gatcgaaagg tggagtctac gatcatcatg
     4141 atgtctgctt atggaagcct ggacaccgcc gtggaagcca tgaagagggg cgcctatgac
     4201 tatattacca agccctttaa acccgatgaa atcctgctgg ctctgcgcaa agcggaggaa
     4261 cgggagaccc tgaggaaaga aaacgctcag ctgcggcagg tcgtt
//
LOCUS       DASPRR010000074         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2116652, whole genome shotgun sequence.
ACCESSION   DASPRR010000074 DASPRR010000000
VERSION     DASPRR010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116652"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1126
                     /locus_tag="VEL68_02665"
     CDS             356..1126
                     /locus_tag="VEL68_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ34902.1"
                     /translation="MKLQNKVAIITGGGRGLGRAVALLFAKEGAKVVLAARSLEEIEH
                     VATELRALRKEALPVPTDVSDEGQVREMVKRTLEAFGTVDILVNNAGARGPIAPIHEI
                     PLAEWEQALKVNLTSAFLCSRAILPTLMEKKKGKIINVTTTLTPRPNLTPYMVAKAGL
                     IHFTKQLSRELKEYNIQVNSIHPGVMDTRMQEEIRKAGAKAIGTDMFERLKEEGMLHT
                     PDEPAKLVLFLASQAADGITGEYLSFDDKEVKFLISQM"
     gene            1270..2388
                     /locus_tag="VEL68_02670"
     CDS             1270..2388
                     /locus_tag="VEL68_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXZ34903.1"
                     /translation="MLKSLVKFFLGDRREFDTFQVEVSTFSSLECQVCARAAFAEKWV
                     FQNMSLETFQRIRPHLPRTHWVSFRGWGDPLENENFLEMVRQVKEAKCAAALTTNGSL
                     LTEEYSGQLLRTDPDLIVVSLELATQAVQDSLARMGSDLRRILDQVQALVKLRKESGR
                     KKPAIKLSFPMTRLNMGELPGLVPLSAKLGVDEIIFTNLDYLPEERWNILRTFHHESP
                     TQAFQDHADEVQQLGKTERIAVKVYPLKAQEVPVCEADPPQKIFFSVEGSISPCQYLR
                     IPKQGEIPRIFMNKECAVPQMIFGNVNQEDFPDVWNKESYQAFRKIFEDRKRAALNAE
                     EVLDSFSNFDSGMDTEPAHEPPPPLSEACRTCYKAYGI"
     gene            2425..3195
                     /locus_tag="VEL68_02675"
     CDS             2425..3195
                     /locus_tag="VEL68_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXZ34904.1"
                     /translation="MQGNLLKKKLKEGQVCFGTFVRLGPAATEILGYAGWDFAVIDME
                     HGVFDFPFVEQCIRAARCSGITSLVRIPEPTPSNIMRTVDAGAEGVQVPQVESAETAQ
                     FVCRAARYFPEGNRGLCSFVRAASYSAVPPEEHIASSNREVLTVIHIEGERAVREIES
                     IIETPGLDVIFLGPWDLSQSLGVPGKTKDPRVIELMEKVIALCKKKGIISGTFVRFVD
                     EAKYWIDLGVRYMMLSTDAGLLLQVSRERLEGLRKLSR"
BASE COUNT          823 a          951 c          959 g          761 t
ORIGIN      
        1 cgcgccaggc taaccttagg aaggggctgg tgcgaggtaa gcttgaagga ggatggtttc
       61 atgggccgat cattttgggc ttatgaccca cttggaaaat gatatccatt tatacttacc
      121 ggattcaagg aaagaagtca aaatagatac cttccccttt cccccgatca tctctcaaag
      181 ccggaccgtc ttggaacctg atgaacattt gaacattggg cctggaatac ggtatattta
      241 tgcataatcg gggagggtat tccttgaaac gccccttgaa gatgcgcagt cctgcaggcc
      301 attcgagtga ccatcaagga actggtttcg cagagacctg cggcctggag gagccatgaa
      361 gctgcagaac aaagtggcca tcatcaccgg aggaggaaga ggattgggcc gggcagtagc
      421 ccttctgttt gcaaaggaag gggcgaaggt ggtcctggcc gccaggagcc tggaagagat
      481 cgaacatgtg gccacggagc tcagggcttt gaggaaagag gcccttccgg tcccaaccga
      541 tgtttccgat gaagggcagg tccgcgagat ggtcaaaaga acgctggagg ccttcggcac
      601 ggtggacatc ctggtaaaca acgccggagc ccgcggaccc atcgctccga tccatgaaat
      661 ccccctcgca gagtgggaac aggcgttgaa ggtcaattta acttccgcct tcttgtgttc
      721 cagggctatt cttcccacac tgatggaaaa gaaaaagggt aaaatcatca acgtgaccac
      781 caccctgacc cccaggccga atttaactcc ttacatggtt gccaaagcgg ggctcatcca
      841 tttcaccaaa cagctatctc gggaattgaa ggagtataat atccaggtca attcgatcca
      901 ccccggagtc atggataccc gcatgcagga ggagatccga aaggcaggag ccaaagccat
      961 cggcacggat atgttcgagc gtttaaagga agaggggatg cttcatacgc ctgacgaacc
     1021 ggccaagttg gtcctctttc tggcctccca agcggctgac ggcatcaccg gggaatatct
     1081 ctcttttgac gataaagaag tgaaatttct gatctcccaa atgtagggct ggcccccgtt
     1141 tccgtcacgc catggactga tgggttcgag gaaggtaccc tacaattcgg tctatcgctg
     1201 aaggcgaaca ccgctttctt tttacgccat gccctatgct ctatgcccaa tgcgtttttt
     1261 caggtgaaca tgctgaaaag tctggtcaaa ttttttctgg gcgaccggcg ggagtttgac
     1321 actttccagg tcgaggtatc caccttcagc tccctggaat gccaagtttg tgcgcgagcc
     1381 gcctttgccg aaaaatgggt ctttcaaaac atgtccctgg agactttcca gaggatccga
     1441 ccgcacttgc cccgcaccca ttgggtttcc ttccgaggct ggggagaccc cctggagaat
     1501 gaaaacttcc tggaaatggt ccggcaggta aaggaagcca aatgtgcggc agccctgacc
     1561 actaacggct cccttttgac cgaagagtac tccggtcagt tgctccggac cgaccccgat
     1621 ttgatcgtcg tgtccctgga actggccacc caagcagttc aggacagcct ggcccgaatg
     1681 ggctctgatc tcaggcgaat cctagaccag gtccaagccc ttgtgaagct gagaaaagag
     1741 tcggggcgga aaaaacctgc catcaaatta tccttcccca tgacccggct taacatgggt
     1801 gaactgcctg gcctggttcc tctatcggcc aagttgggag tggacgagat cattttcacc
     1861 aatttggact atctgcccga ggaacgatgg aacatccttc gaaccttcca ccacgaatcc
     1921 ccaacccagg cattccagga tcatgccgat gaggttcagc agctgggaaa aacggaaagg
     1981 attgccgtaa aggtatatcc cttgaaggct caagaggttc cggtctgcga ggcggaccct
     2041 ccccaaaaaa tctttttttc ggtggagggt tcgatctccc cctgccagta tctccggatt
     2101 cccaagcagg gagagattcc ccggatcttt atgaacaagg aatgtgccgt cccccagatg
     2161 atcttcggca atgtcaatca agaagatttt ccagacgtat ggaacaagga atcgtaccaa
     2221 gccttccgca agattttcga ggaccggaaa agagccgcct tgaatgcaga ggaggtcctg
     2281 gattcctttt ccaatttcga ttcgggaatg gacactgagc ccgcccatga gcctccccct
     2341 cccctttccg aggcatgccg gacctgctat aaggcctatg gaatctagaa ccaaaatgca
     2401 agctcaatcg taaaggagaa acctatgcag ggcaaccttt tgaagaaaaa gctgaaggaa
     2461 ggacaagttt gcttcgggac ttttgtccgt ctcggcccgg cggcaacgga aatcctgggt
     2521 tacgctgggt gggattttgc ggtcatcgac atggaacacg gagtctttga ctttcccttc
     2581 gttgaacaat gcatccgggc cgcccggtgt tccggcatca ccagtctggt gcggatccct
     2641 gagcccactc cgtcgaatat catgcggacg gtggatgccg gcgccgaagg agtccaggtt
     2701 ccccaggtgg agagcgccga aacagcccaa ttcgtctgcc gggcggcccg ctattttcca
     2761 gaggggaatc gagggctctg cagttttgtc cgggccgcaa gttactccgc cgttcctccc
     2821 gaggaacata tcgcctcctc caaccgggaa gttctgaccg tcatccatat cgagggggag
     2881 agagcggtgc gggaaatcga gtccattatc gagacgccgg ggctggatgt catattcctt
     2941 gggccctggg acctgtccca atccctgggg gtcccgggaa aaaccaagga cccgcgggtg
     3001 atcgaattga tggaaaaggt gatcgccctg tgcaagaaaa aggggatcat ctccggcact
     3061 ttcgtgcgtt tcgtggacga ggccaaatat tggatcgacc ttggcgtccg ctacatgatg
     3121 ctctccacgg atgccggatt gctcctgcag gtgagccggg agcggctgga aggattgcga
     3181 aagctttccc gctaacctgc cttgggccct tcccggcccg gatgatcttg gagggagaac
     3241 ccggctctcc tggcttgccc acccccagtt tttcaagctt tttggggttc agggtggagg
     3301 aatttggatc atcaggctcg gcgggtgctt atccttccat caaactcctc cccatgttcc
     3361 gcagggggag gcctgagagg agattcctcc aagcagaaaa gagaaccaaa gcatgtttcc
     3421 gtttcaatcc ccccaggaca ggcccgatcc catggaagaa ccaccgcctc aggatgatga
     3481 tccaaatttc tctg
//
LOCUS       DASPRR010000075        12248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_23112, whole genome shotgun sequence.
ACCESSION   DASPRR010000075 DASPRR010000000
VERSION     DASPRR010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12248)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12248
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23112"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(704..1657)
                     /locus_tag="VEL68_02680"
     CDS             complement(704..1657)
                     /locus_tag="VEL68_02680"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXZ34905.1"
                     /translation="MMKDLISISEKLLGDCLNLKGGETFLVVTDRMKREIGAALYQAG
                     FNLGAEAMLLEMKTRTRNGEEPPLPVAEAMISSKVVVCPTQYSLTHTQARKRAVEKGA
                     RVATMPGITEDMFGQGAITADYREVEELTNKMTDILTRGKEVRIMKEGRELRFSIAGR
                     KGIASTGIYREPGQSGNLPSGEAYIAPLEGTAEGKILVDGSMVGIGLLKDPLEITISA
                     GKLTRVEGRRAKDFLTSLGASEPARNVAEFGIGTNRKARLTGIILEDEKIYGTVHVAF
                     GDNSTFGGSVQAGIHLDGVILKPDVYVDDAKIMDQGEIINP"
     gene            complement(1673..2353)
                     /locus_tag="VEL68_02685"
     CDS             complement(1673..2353)
                     /locus_tag="VEL68_02685"
                     /inference="COORDINATES: protein motif:HMM:NF007788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HXZ34906.1"
                     /translation="MRKGKKGIGVVTIGQSPRTDVLPEMKLFWGREVEVRECGALDDL
                     SRKEIQDLGANIKGDLLVTRLRDGTEVKVGHEDVIPRVEACIRKLENEVPVIILICTG
                     TFPPFKSKSFILYPEPILYHVAQSVLRGGKLGVLTPGVEQIQYQQERWMKIFPEVSVR
                     GASPYVKDGEEVKEAARKLKEEGSNLIVMDCIGYTLMMKDWVKEVTGKPALLARSTLA
                     RVAGELLG"
     gene            complement(2350..3288)
                     /locus_tag="VEL68_02690"
     CDS             complement(2350..3288)
                     /locus_tag="VEL68_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HXZ34907.1"
                     /translation="MALKQVLEITDLLDRAAITGEGVASFFRQRGVKNLEVQTVRGEK
                     GKTDFVKFLIPGTKGKEGKGDAPTLGVIGRLGGLGARPNVLGLVSDSDGAVCALSICL
                     KIIDMQAKGDFLSGDVIVATHICPDAPTQPHEPVPFMGSPVDMATMNRLEVDKRMDAI
                     LSFDTTKGNRVINHRGFAISSTVKEGYILRISEDLLEIMQITTGRLPVVFPITMQDIT
                     PYGNGLFHLNSILQPCIATDAPVVGVAITAEVAVPGCGTGASHEVDMEQAVRFGVEVA
                     KTFGKGSLRFYDPEEFSRCIALYGSMKHLQGMGTKR"
     gene            complement(3299..4366)
                     /locus_tag="VEL68_02695"
     CDS             complement(3299..4366)
                     /locus_tag="VEL68_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 family protein"
                     /protein_id="HXZ34908.1"
                     /translation="MILDRKTVEAMVFGGGFLGGGGGGSMEEGRRMGLLALEVGSPRL
                     IPIDSLSPDAVLVTVSAVGAPGAPEKSVKPVDFVEAVKVLSGHLGAKIAGFISSENGG
                     ISTTNGLIQSAFLGIPLVDSPCNGRAHPTGIMGSMGLNRQPDFISQQAAIGGNPQKSR
                     RISIYVEGNLVQADGLIRQAAVEAGGMIAVARNPIAASFVRENGAPGAISQAIELGQF
                     IMADKSNPRTLAERIAKRFSGEVITEGEISSYQMETRGGYDVGRIEIGGFELTFWNEY
                     MTLEKKGERIATFPDLMVTLDAEKGFPLSSSELSRDRRVIVIHVPRQNLILGAGMKEK
                     SNFIKVEEVLRKEVIRYIFPS"
     gene            complement(4465..5118)
                     /locus_tag="VEL68_02700"
     CDS             complement(4465..5118)
                     /locus_tag="VEL68_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXZ34909.1"
                     /translation="MARRVVGIIRVVTLTDQEIMNRHGKLLESLFPDFTTVTECIPNQ
                     PNGIHNEETETTAIPKIIALGKKMAKRVQALIISCAADPAVEDLRRVLSIPVIGAGSA
                     AVGVGIALGSRIGVLNLNEETPRVISSLLGKMLIGATSPEGVNTTIDLMTPVGKTAAV
                     KAANRLGQIGADAIVMACTGYATISLAPVLRKESKIPIVDPIEASGLVAHEVLRYRA"
     gene            complement(5236..6867)
                     /locus_tag="VEL68_02705"
     CDS             complement(5236..6867)
                     /locus_tag="VEL68_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HXZ34910.1"
                     /translation="MEKEEEGKEKLEVVELEIPAKHPRAIEANVMIFNIILSVIGAII
                     GLQILTTLGITPNTAIIGVLLAIAISRIPVPFLSAFRSIHRQNLLQSNISSATFGAAN
                     CLLLPIGIPFLLGRQDLVLPMLLGATMGMLVDLAMLYWFFDSRIFPGENAWPPGLAAA
                     EAIIAGDRGGKRGRLLIYGTVVGVIGSYFNISMSAFGVAFIGNIVALAMFGVGLLIRG
                     YAPALFHVDINKLYIPHGMMIGAGLVALIQIIVILTKRTGKESGATTQPVSYTRSENY
                     VRRKLVTGCVLYLIAAIIVSLATGLWAEMSFGKILLWVVFAAFACVAAEFIVGLASMH
                     AGWFPAFATALIFLVLGILMGFPPVAAAILVGFVASGGPAFADAGYDLRAGWWLRGKG
                     KHLQFELDGRWQQIIAGFVGLMIGLVMVFFFRDLYFSQNLVPPVDRVFVATIKAGVDP
                     SLIKSLLIWAMPGAIIQILGGSTRQMGILLSTGLLIMNPLAGWAVLVGIAIRYALRTK
                     YGEEMETPMTIFGAGCIAGDALYGFFNSVFKAKWR"
     gene            7232..8335
                     /locus_tag="VEL68_02710"
     CDS             7232..8335
                     /locus_tag="VEL68_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HXZ34911.1"
                     /translation="MKAKAAIFWEVGKKLDIQEVDVEKPHAGEVLVKLAAAGICHSDY
                     HVMTGHLFGPSPAILGHEGTGIVAEVGPGVTAVKPGDHIIIMWRLSCGICEYCSDGRP
                     ALCPAGAQIRSTGKLLDGTTRFKYKGQEIKHFAGASTFSEYTVIPEGSALKIPQDFPL
                     DIAALLGCGVITGVGAAVNCAKVKPGSTSVVIGTGGIGLNIIQGAAIAGASKVIAVDV
                     NAKKLDYAKTFGATHVIDATKKNAVEAVKALTNGAGVDYAFEAVGLPETIRQAFDCLA
                     KRGMCVAVGVAPMTKEVAVPIMALVFEEKVLTGSLYGSARIRQDTNMLMEMYKAKKLK
                     LDELLTRKYPFVQINEAYDNLIKGEVARSIVTF"
     gene            complement(8403..9455)
                     /locus_tag="VEL68_02715"
     CDS             complement(8403..9455)
                     /locus_tag="VEL68_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ34912.1"
                     /translation="MKAALWHGRGDVRIENVPEPTPKAGEVKIAVKWCGICGTDVGEF
                     LNGPVLIKNPPVILGHEYSGEIVAWGEGVQGFRKGDRVTAMNVRSCGQCANCKVRSSM
                     ALGKKMELCENMDTTGLTSTAPGAFAEFLCIPDYLAVKFDDALSWEEGTMINPLSIGI
                     HAIKRGNLRLGETAVIIGDGTIGQLALQACIACGTRAAFLVGEQPLRLELARRGGAHG
                     VFHYQEKNLKEEIRELLGGRLPEVTFDCVGSETSLQTALSLAAKGGRVLLVGIYERPC
                     RVDCKNIVVQEKTLLGVLSQDYEDYFIGNALLSQKKVASTPLITKKISLKDLVDGGIK
                     ELIANRKEQLRILVHP"
     gene            complement(9510..10721)
                     /locus_tag="VEL68_02720"
     CDS             complement(9510..10721)
                     /locus_tag="VEL68_02720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXZ34913.1"
                     /translation="MAVVIILPKIDEAMTKGKIVEWKKKEGDRVTKGEVVFIIETEKV
                     TWEVEAPESGILQQVRYGVGEEVAVGTAVAFILGEGETWSEKYLDQVAVREIASSAGV
                     ERRDSPPAPEGFPQRRVRATPLAKRIAREQKINLLDIRGSGPGGRITGKDLRQAPGDG
                     QKRVPPLAVPATPFTQGKKIPLSSMRETIARRLTESFQSVPHFYLSLEEDVEKLCQAR
                     EDLTPLIEKESGVRISLTDLLLKITASALAEHPEINVQWAGDGIRHLAEINIGLAVAL
                     DTGLVVPVVRLANSLSLADIARVRADFVKRAQQGKIRMEEMRGGSMTITNLGPLGIDQ
                     FYPIINPPESCILAAGRVLEKPVAHQGQVVIRKRMSLTLAIDHRVLDGATGSRFLGTI
                     QKLMEQPLLLI"
     gene            complement(10753..11730)
                     /locus_tag="VEL68_02725"
     CDS             complement(10753..11730)
                     /locus_tag="VEL68_02725"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXZ34914.1"
                     /translation="MREKTYREALREAMREEMRRDESVFLMGEDIAHFGGAYKVTLGL
                     SDEFGKKRVLDTPLAEAGIMGAAIGAAIIGMRPVAEIMYIDFSTIASDLLINLAAKFR
                     FMSAGTFKLPLVVRTQGGGGVSAGPQHSQSLEAIYAHIPGLMVVMPSTPYDAKGLLKS
                     SIREDNPVVFIEHKGLYGMKGPVPEEEYLIPLGKADIKRQGRDITVVTYSRSAIHTME
                     AASFLEKEGISLEVIDPMTLKPLDEETILESVQKTGKLMIVHEAHKTGGFGAEIAALV
                     SEKAIDCLDAPVRRIAALDVPIPFGLKMEGFVLPNVEDIVAGARELAKW"
     gene            complement(11742..>12248)
                     /locus_tag="VEL68_02730"
     CDS             complement(11742..>12248)
                     /locus_tag="VEL68_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ34915.1"
                     /translation="DLPVLFVCQNNQYEEGTHWTYWGGRLKSLADRSSGYGIPGFSVY
                     GNDAVAVAEAAAEAVEKVRKGGGPILLEGKTYRIHGHHMGDPGTTYRTKKEVEEWKGK
                     DPIHRLYSLLFQKGLITAEENSKIEVQIEQELDEAVQFALESPEIDPHTTFRDLVYFE
                     RPVGLNKI"
BASE COUNT         2666 a         3624 c         3054 g         2904 t
ORIGIN      
        1 ctaatagtcc ggcggaccga ttccttaatg ccttgagcct tggatgtcgt tccttcggta
       61 tccttgacca tctcacgccc ttattctgaa ccttcctgat ggcccctttc cttttgggaa
      121 tgtgcatttc atcatggtaa acccccggct ctgccgaggg tccgcctgtg gcggattgac
      181 atttccggaa tgcctcggtt gtggcccggg ggcaaggagc ttctgggcaa aaagcatcgg
      241 ccgggctgcc ctcttctggg aggcttcggc cggcttccgc agaatgcggc gggctgaccg
      301 gggaaccggc cggtgaccct acgctgagag aacatcttca ggggggttcg ggtgctggcc
      361 cgcccggtcc atgaaatgga agctgcctca tggtttttgc ccagaaggtc cttgcccccg
      421 gacctggcac ccaattgagg gccaccttta ggtggcgccc ctttcaatcc gctttgagcg
      481 gttcactgtt tttcatacct ccggctctgc cggaggtcat gattaaagat tgatcttggg
      541 cggaatatta tggcgaaacg gcatctttcg attgacgatt ccattaaacg aatccagccg
      601 gagattcggc gctgcacgca ttttcagcct tacgatagtg gggtgaatag aaaaggagga
      661 ttttaaaggt tttcaaaagg ctacgctgct atctccaatg catctaggga ttgatgattt
      721 ccccctggtc cataattttg gcatcatcca catagacatc tggttttaag atcacaccat
      781 caagatgaat tccggcttgc accgaacccc caaaggtaga gttatcaccg aacgcaacgt
      841 ggaccgtacc gtatatcttc tcatcttcca ggatgatacc cgtaagccgt gccttccgat
      901 ttgtcccgat cccaaattcg gctacatttc tggccggttc agaagcaccc agagacgtaa
      961 ggaaatcttt ggcccggcgg ccttccaccc gggtcagttt cccggctgag atggttattt
     1021 ccaggggatc cttgagcagt ccgattccga ccatggaacc atcgaccaga atttttccct
     1081 ctgcggttcc ctccaggggg gcgatgtacg cttccccgga gggcaggttt cctgactgcc
     1141 ccggttctcg atagataccg gtgctggcaa tccctttcct cccggcaatg ctgaagcgga
     1201 gttcccttcc ctctttcata atccgaacct ccttacccct cgtcaggatg tcggtcattt
     1261 tattggttaa ctcttccacc tcgcgataat ctgcggttat ggcaccttga ccgaacatat
     1321 cctccgtgat tcccggcatg gtggcaaccc gcgccccctt ttcaacggcc ctcttccgtg
     1381 cctgagtatg ggtgagggaa tactgggtgg gacaaacgac gaccttcgag gatatcatgg
     1441 cctcagccac gggcaaagga ggttcttccc cattccgggt gcgggttttc atctccagaa
     1501 gcatggcctc tgctccgaga ttgaaaccag cctgatacaa ggccgctccg atttccctct
     1561 tcatacgatc ggtcacgacc agaaaagtct ccccgccctt caagtttaga caatccccaa
     1621 gcagcttctc actgatggat atcaggtctt tcatcatagc ccccgtcctt gcttatccta
     1681 aaagctcacc ggcaaccctc gccagagtgg accgggcgag aagggcaggt ttccccgtaa
     1741 cctccttcac ccaatctttc atcatcaagg tataaccgat acaatccatg acgatcaggt
     1801 tcgaaccttc ttcctttaac ttccgggccg cctcctttac ctcttccccg tctttgacat
     1861 aaggagatgc accccgtacg gacacttcgg gaaatatctt catccatcgc tcttgctgat
     1921 attggatttg ctccacccct ggggtcaaga cgcctaattt tcccccccgg agcacgctct
     1981 gggccacatg ataaagaatc ggctctggat agaggatgaa actttttgac ttgaatgggg
     2041 ggaaggtgcc cgtacagatc aagatgatta ccggaacctc gttctccaat ttacggatgc
     2101 aagcttccac ccgaggaatg acatcttcat gtcccacctt gacctcggtt ccatcccgaa
     2161 gacgggtcac cagaaggtct cctttgatat tcgcccctaa atcttgaatc tcttttcggc
     2221 tgagatcatc cagggcaccg cattcccgaa cttcgacctc tcttccccaa aagagcttca
     2281 tttcgggtaa gacatcggtc ctgggagatt gaccgatcgt gaccactcct atccccttct
     2341 tccccttcct catctcttgg tccccattcc ctgtaaatgt ttcatggagc cgtaaagagc
     2401 aatgcatctt gagaattctt ccgggtcata gaatcgcaga ctccccttcc caaaagtctt
     2461 ggctacttca accccgaacc gaaccgcctg ctccatatcg acctcatggc tcgctccggt
     2521 tccacacccg gggaccgcca cctcggcagt gatggcaacc cccaccacag gcgcatcggt
     2581 cgcaatacag ggttgcagga tgctgttcag atggaaaagg ccgtttccat aaggggtgat
     2641 atcctgcatc gtgatgggga aaaccaccgg caatcttccg gttgtaattt gcatgatctc
     2701 aagcaggtct tcgctgattc tcaggatata accctccttg accgttgaag agatggcaaa
     2761 gccccggtgg ttgatcaccc ggttcccctt ggtggtatca aaagagagaa tggcgtccat
     2821 ccttttgtcc acctcgagcc gattcatcgt ggccatatcc accggagacc ccataaaggg
     2881 cacgggttca tggggttggg taggagcgtc agggcagatg tgggttgcca caatgacatc
     2941 cccgctcaga aaatcgcctt tggcttgcat gtcgattatc ttcaggcaga tagatagagc
     3001 gcagaccgcc ccgtcgctgt cggaaaccaa tccgagcaca ttcggcctgg cccccagtcc
     3061 acccaaacgg cctatgaccc ccagggtcgg ggcatctcct ttcccctcct tgcctttggt
     3121 ccccgggatg agaaacttta caaaatcggt tttgcctttt tctccccgaa cggtttgcac
     3181 ttccaaattt ttgacccctc tttggcgaaa gaaagaggct accccttcgc cggtgattgc
     3241 cgcccgatcc agaagatcgg ttatttccag aacttgtttc agagccattt cttccctcct
     3301 acgatggaaa aatataacgg atcacttctt tcctcaaaac ctcctccacc ttaatgaaat
     3361 tcgatttctc tttcatcccc gccccaagaa tcaagttttg gcgcggcaca tggattacga
     3421 taacccgccg gtcccggctt agctcggagg aggaaagggg gaatcctttc tccgcatcga
     3481 gggttaccat aagatccggg aaggtggcga tcctctctcc tttcttctcc agggtcatgt
     3541 actcgttcca gaaggtgagt tcaaatcccc cgatttcaat cctacccaca tcatatcccc
     3601 cgcgggtttc catttgataa gaggagattt ctccctcggt gatgacctcc cccgagaatc
     3661 tcttcgcaat cctttctgca agggtcctgg ggttcgactt atcggccatg atgaattgtc
     3721 caagttctat cgcctgggag atggcgcccg gcgccccatt ctcccgcaca aaagaagcag
     3781 caatgggatt ccgcgcgacg gcaatcattc cccccgcttc cacggcggcc tggcggatga
     3841 gcccgtctgc ctggacaagg tttccttcca cgtaaatgga aattctccgg cttttttggg
     3901 ggttcccgcc tatcgcggct tgctgggaga tgaaatcggg ttgacgattc aaacccatgg
     3961 accccatgat tcccgtcgga tgggcccttc cattgcaggg ggagtccacg aggggaattc
     4021 ccaggaaggc agactggata agcccgttgg tagtagaaat acccccattc tccgatgaga
     4081 taaatcctgc aatcttcgct ccaagatgac ctgataggac cttgaccgct tccacgaagt
     4141 cgacaggctt cacagatttc tcgggcgcac cgggagcccc aaccgcagaa accgtcacca
     4201 ggacggcgtc cggggatagg gaatcaatcg ggatgagcct gggactccct acctccaggg
     4261 ccaggagacc catccgccgg ccttcttcca tcgatccgcc gccaccgccc cccaaaaaac
     4321 ctccgccgaa aaccatcgcc tccactgttt ttctgtccaa tatcattccc cgctcacctc
     4381 cgatgaagat atggatactc ctctaagcct cccgacggcc caaattttag cgccttaccc
     4441 gcaggaagtg gaaaacgatg aacttcatgc ccggtaacga aggacctcat gggcgactaa
     4501 acctgaggcc tcaatcgggt cgacgatagg aattttgctc tcttttcgca agacgggagc
     4561 gaggcttatc gtagcgtagc ccgtgcaagc catgacgatg gcatccgccc cgatttgacc
     4621 caggcgattg gcggccttca cggctgccgt tttccccacc ggggtcatga ggtcaatcgt
     4681 tgtgttgacc ccttccggac tggtcgctcc tatcaacatc tttccgagca aggaagatat
     4741 gaccctgggg gtttcttcgt tcagattcaa caccccgatt cgactcccca gcgctatccc
     4801 cacccccact gcggcagagc cggccccaat taccggaata ctcagaactc tacgaaggtc
     4861 ttcaaccgcc gggtccgctg cacagctgat gattaaggct tgcacccgtt tagccatctt
     4921 ttttcccagg gcgataattt tagggatggc cgtggtttcg gtctcttcat tgtggatccc
     4981 gttgggctgg ttggggatgc actccgtaac tgtagtaaaa tctgggaaaa ggctctccaa
     5041 taattttcca tggcgattca ttatttcctg gtcggtgagg gtgaccaccc ggattatgcc
     5101 tacgactctt cttgccattt cctaacctcc tgacctggca tattccgcca aacaagatat
     5161 tgtggggggc acgcgcttgg cgtgattgcc gccataactt gtgactattt ggcggtccat
     5221 ttgatcggta agggatcaac gccatttggc tttgaaaacc gagttaaaga acccgtagag
     5281 agcgtccccg gcgatacatc ctgcaccaaa gatcgtcatg ggagtctcca tctcttctcc
     5341 gtattttgtt ctgagcgcat atcggatggc aataccgacc aaaaccgccc aaccggccag
     5401 gggattcatg atgagaaggc ctgttgacag cagaatcccc atctgccgag tcgaaccacc
     5461 caaaatctga ataatcgccc ccggcatggc ccagatgagg aggcttttaa tcagcgaggg
     5521 gtctactcct gccttaatcg tagctacaaa tacccggtca acgggtggaa caaggttttg
     5581 ggagaaatag agatcccgga agaaaaaaac cattaccaga ccaatcatca accctacaaa
     5641 tccggcaatg atttgttgcc accgtccatc gagttcaaat tggaggtgtt tcccctttcc
     5701 tctaagccac catccggccc ttaggtcata tcccgcatcc gcaaatgcgg ggccaccgga
     5761 ggccacaaag ccaacgagaa tggccgccgc cacaggcgga aaacccatga gaatacccag
     5821 gacgagaaag atgagagccg tggcgaaggc cggaaaccag ccggcgtgca tggacgcaag
     5881 gcctacgatg aattccgcgg ctacacaggc aaaggctgca aaaacgaccc acaacaatat
     5941 tttcccgaaa gacatctctg cccataaacc cgtcgcgaga gacacaatga tggctgctat
     6001 cagatagaga acacagcccg ttacgagttt acggcgtaca taattttcgg agcgcgtata
     6061 gctgacgggt tgggtcgtcg ctccggattc ttttcccgtc cttttggtca ggatgacaat
     6121 aatctggatc agggcgacaa gccccgcgcc gatcatcatt ccatggggga tgtataattt
     6181 gttgatatcg acgtgaaata gggccggggc gtaaccccgg atcaggagac cgactccgaa
     6241 catcgccaaa gctacgatgt tcccgataaa agcgactcca aaggcagaca tggaaatatt
     6301 gaaataagag ccgatgacgc cgacaacggt gccgtagata agcaacctcc ccctcttgcc
     6361 cccccggtcg ccagcgatga ttgcttccgc agcagccagc cccggcggcc aggcattttc
     6421 gccggggaat atgcgagagt caaagaacca gtagagcatg gccaggtcta ccagcattcc
     6481 catggtggct cccaaaagca tggggagaac caggtcctgc cgccccaaga ggaaggggat
     6541 gccgattggt aaaagcaagc agttcgccgc gccaaaagtc gcgctggaga tattcgattg
     6601 taacaggttt tgccgatgga ttgacctgaa ggcacttaaa aaaggaacgg gaattcttga
     6661 gatcgcgatt gccagaagga cgccgatgat tgcggtattc ggggtgatcc ccagggtggt
     6721 gaggatctgc aggccaatga tcgctccgat aacggaaaga atgatgttga agatcatcac
     6781 attggcttct atggcccttg gatgcttggc aggtatttct aactccacga cctcaagttt
     6841 ctctttgcct tcctcttctt tttccataga gtcacctccc attttagagt ttggccaact
     6901 tgcgcccccc aaaaaataaa gcctggctat ccttttcaga agtccaggct ttctaataat
     6961 ttaccgttct tctcaccacc tatggatgaa agaggggggg gtcgatcaaa aaaggtaatg
     7021 aattattttc cgcgaaaacc taatccatga acacctcatc aagggaattt ctcccttcat
     7081 acatccagcc aaaatctttg tcaaggtctt aaaagacttt accccctcgg ccctttttgg
     7141 ttaattcttt tgcatgaaaa aaccctcccc tttttatttc ttttttagta tgatggtccc
     7201 ggccgcaata ttatgaaagg gaggttctat tatgaaagcg aaagcagcga tattttggga
     7261 agtgggaaag aagttggata tccaggaagt cgatgtggag aagccccatg ccggggaagt
     7321 tctcgtcaaa ctggccgccg cgggcatttg ccacagcgac tatcatgtga tgaccggcca
     7381 tcttttcggc ccctctccgg ccattcttgg acacgagggc accggcatcg tcgctgaagt
     7441 cgggccgggg gtgactgccg tgaaacccgg agatcatatt atcatcatgt ggcgtctttc
     7501 ctgcgggatc tgcgaatact gcagtgatgg gcgtcccgcc ctctgtccgg caggcgctca
     7561 gatccgttcg accggcaagc tcctcgacgg gaccacccgt ttcaagtaca aaggtcagga
     7621 gatcaaacac tttgccgggg cctccacctt ttcggaatac acggtgattc ctgagggatc
     7681 cgccctcaag attccccagg attttccctt ggacattgcc gccctcctgg gatgcggggt
     7741 catcaccggg gttggcgccg ccgtcaactg cgccaaggtc aagccgggaa gcacctcggt
     7801 tgttattgga accggcggaa ttggtttgaa tatcatccaa ggtgcggcta ttgcgggtgc
     7861 gtcgaaagtc atcgctgttg atgtcaacgc gaaaaaactc gattacgcca agacctttgg
     7921 agccacgcat gtcatcgacg ccacgaagaa gaatgccgtg gaggcggtca aggccttgac
     7981 caacggcgca ggggttgatt acgccttcga ggcggtcggg ttacccgaga cgatccgcca
     8041 ggccttcgat tgcctcgcca aacgagggat gtgtgtggcc gttggcgtgg cgcccatgac
     8101 gaaagaggta gccgttccca tcatggctct cgtattcgag gaaaaggtat tgacgggatc
     8161 gctctatgga tcggcaagaa tccggcagga caccaacatg ctgatggaaa tgtacaaagc
     8221 gaaaaagtta aagctcgacg aacttctcac ccggaaatac cctttcgtcc agatcaacga
     8281 ggcttatgat aacctcatca agggcgaagt ggctcgcagt atcgtgacct tctgagattc
     8341 gaatcttggc caagtggcat gggaaattct ggcctgtttc ccatgccaac cctttggttt
     8401 attcacgggt gtactaagat acgaagctgc tccttccgat tggcaataag ttccttgatc
     8461 ccgccatcga ccaggtcctt gagggagatt ttcttggtaa tcagcggggt tgaagccact
     8521 tttttctggg aaagaagggc attcccaatg aaatagtctt cgtaatcctg cgaaaggacc
     8581 cccaacagcg tcttctcctg gacgacgatg ttcttacaat ccactcggca ggggcgctca
     8641 tagatcccga ccagaagtac cctcccacct ttcgcagcca gacttaaggc cgtttgtagg
     8701 gaagtttcgc tgcccacgca atcgaaggtc acctccggca gccgtcctcc caaaagctcc
     8761 ctgatttctt cctttagatt cttttcctgg tagtggaaca ccccgtgggc cccccctctt
     8821 ctggcgagtt ccaatcgcaa aggctgctcc ccgaccagaa aagccgcccg cgtgccgcag
     8881 gcaatgcacg cctgcagggc aagttggccg atggttccat ccccgatgat cacggcggtt
     8941 tcacccagcc tcagattccc ccgtttgatt gcatggatcc cgatgctgag gggattgatc
     9001 atggtgcctt cctcccagga gagggcatcg tcgaatttca ccgcgaggta atctggaata
     9061 cagaggaatt cggcaaatgc ccccggggca gtggaggtta gccccgtcgt gtccatattt
     9121 tcgcaaagct ccattttttt ccccagggcc atcgagcttc taaccttgca gttggcgcat
     9181 tggccgcagg acctgacatt cattgcggtg acccggtccc ctttcctgaa cccctggaca
     9241 ccttctcccc aagccacaat ttccccagaa tattcatgcc ccaggatcac ggggggattc
     9301 ttaatcaaaa ccgggccgtt caggaattcg ccgacatccg tgccgcaaat gccgcaccat
     9361 ttaaccgcga tcttcacttc ccctgctttc ggggtaggtt ccggaacatt ttcaatcctg
     9421 acgtccccac gcccatgcca taatgccgct ttcattgttt cctctcatgg ccaccgattt
     9481 tggaaaggtc attcaccgaa ggggaatcgc tagatcaaca acaagggctg ctccataagc
     9541 ttctggatgg tccccagaaa ccggcttcct gtagccccgt ccaagacccg atgatcaatg
     9601 gcgagggtaa ggctcattct cttgcgaatc accacctgcc cctggtgggc caccggtttc
     9661 tccaaaaccc gccccgccgc cagaatgcag ctctccgggg ggttgataat cgggtagaac
     9721 tggtcaatgc ccaatggccc cagattggtg atcgtcatgc tgccgcctct catctcctcc
     9781 atgcgaatct tcccctgctg agcccgtttt acgaagtcgg cccggaccct cgcgatatcg
     9841 gctaaagaaa gcgaattagc gagacgaacg acagggacga ctaaaccggt gtcgagtgca
     9901 accgccaggc cgatattgat ctccgcgagg tgccggattc catcccccgc ccactggaca
     9961 ttgatttccg ggtgctcagc caaagcgctg gcggttattt tgagcagcag atcggtcagg
    10021 gagatcctca cccctgattc cttttcaatc aggggcgtca aatcctcccg ggcctggcat
    10081 aatttttcca catcctcttc cagagaaagg taaaaatggg gaacggattg gaagctctcc
    10141 gtcagccggc gggcaatcgt ctcccgcatt gaagagaggg gaattttttt cccctgggta
    10201 aaaggagtcg ctgggacggc caggggcggg acccttttct gaccatcccc tggcgcttgc
    10261 cgcaggtcct tcccggtaat ccttccgccg ggtccacttc cccggatgtc caggagattg
    10321 atcttttgct cccgggcaat tcttttggcc aagggggttg ccctcacgcg cctctgcgga
    10381 aatccttccg gtgccggagg agaatccctt ctctcgaccc cggctgagga tgcaatctcc
    10441 ctaaccgcaa cctggtccag gtatttttca gaccaggttt ccccttcacc aaggataaag
    10501 gccacggccg ttcccacggc cacttcttct ccgaccccgt atctgacctg ctgcaaaatc
    10561 cccgattcgg gggcttccac ctcccaggta accttctcgg tttcaatgat aaaaaccact
    10621 tccccttttg ttacccggtc cccctccttt tttttccact cgacgatttt tcctttcgtc
    10681 atggcctcgt cgattttggg caatatgatc acgacagcca tcttccacct cgcgcttgca
    10741 cggctccttg gcttaccatt tcgccagttc ccgggcgccg gcaacgatat cctctacatt
    10801 cgggaggacg aaaccctcca tcttcaggcc gaaggggatg gggacatcca gggccgcgat
    10861 tctcctgacc ggcgcatcga ggcagtcgat ggccttctcg gacaccagag cggcgatctc
    10921 cgccccgaac cctcccgttt tgtgggcttc atggacgatc atcagctttc cggttttttg
    10981 gaccgattcg aggatggttt cctcgtccag gggcttcaag gtcatcggat caatcacttc
    11041 gaggctgatc ccctcctttt ccagaaagct cgccgcctcc atcgtgtgaa ttgcgcttcg
    11101 agagtaagtc accaccgtga tgtctcggcc ctggcgcttg atatccgcct ttcccagggg
    11161 gatcaaatat tcctcctccg gcacgggccc cttcatgcca tacagtcctt tatgctcgat
    11221 gaatacgacg ggattgtctt ctcggatgga tgatttcagt agccctttgg cgtcatacgg
    11281 ggtggaaggc atcacgacca tgagtcccgg aatgtgcgcg tagattgctt ccagggattg
    11341 ggaatgctgg ggaccggccg aaacaccccc tcctccctgg gtccttacaa cgaggggcaa
    11401 tttgaacgtc cccgcgctca tgaagcggaa tttagcggcc aagttaatga ggaggtcgga
    11461 ggcgatagtt gaaaagtcga tgtacatgat ctccgcaacg ggccgcatcc cgatgatggc
    11521 cgccccgata gccgccccca tgatccccgc ttccgctaga ggagtgtcca gcacgcgttt
    11581 tttaccgaac tcatcggaca atcccagggt gactttgtag gcccccccga aatgggcgat
    11641 atcctctccc attaggaaga cggattcgtc cctcctcatt tcctctcgca tcgcttcccg
    11701 caaggcttcc cggtaagttt tttcgcgcac ggctgacctc gtcagatttt gttcagaccc
    11761 accggccgtt caaagtagac cagatcccga aacgtggtgt ggggatcaat ttcggggctc
    11821 tcgagggcga attgaaccgc ctcgtccagt tcctgttcta tttggacctc gatcttcgaa
    11881 ttctcctcgg cggtgatgag acctttttga aaaagaaggg agtaaagccg gtgaatgggg
    11941 tcttttccct tccattcttc cacttctttc ttcgtccggt aggttgtccc gggatccccc
    12001 atgtggtgcc cgtggatacg gtaggtcttc ccttccagga ggattgggcc acctcctttc
    12061 cggaccttct ccacagcttc cgccgccgcc tcggctacgg cgaccgcatc gtttccatag
    12121 acgctgaagc ctggaatccc gtaccccgag gaacgatcgg ccagggattt aagcctcccg
    12181 ccccaataag tccaatgagt cccctcttcg tattggttgt tctggcaaac gaacagcacg
    12241 gggagatc
//
LOCUS       DASPRR010000076         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1955038, whole genome shotgun sequence.
ACCESSION   DASPRR010000076 DASPRR010000000
VERSION     DASPRR010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955038"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VEL68_02735"
     CDS             <1..336
                     /locus_tag="VEL68_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34916.1"
                     /translation="ITSRIGRTARIRGSRDYNSFEVLPPHEMARKKERLRSGDLLFFF
                     KAPGKRVVDEGVGHMGIVKVEGKNSEKEISLIHAGGTKNSGGTVRKVLLVDYLSAMPF
                     VGVKVTRFE"
     gene            416..2473
                     /gene="tkt"
                     /locus_tag="VEL68_02740"
     CDS             416..2473
                     /gene="tkt"
                     /locus_tag="VEL68_02740"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXZ34917.1"
                     /translation="MDLDQLCVNTIRMLSVDMVQEANSGHPGLPLGAAPMAYVLWTKF
                     LKHNPENPKWPDRDRFVLSAGHGSALLYSLLHLTGYDLPLEEIKRFRQWGSKTPGHPE
                     YGDTPGVECTTGPLGQGFAMGVGMALAERFLAAHFNRPGQEIVDHYTYGLVSDGDLME
                     GVASEAASLAGHLKLGKLIYLYDQNSITLAGETRLVFTEDVARRFEAYGWQVQAVENG
                     NDLEAIARAIEKAREENSRPSLISVRTHIGYGSPLKHDTFEAHGSPLGAEEVAATKKN
                     LGWPLEPKFFIPEEVRAHFHKITAQGAKIEEDWEAKLRSYQTAFPELRKHWDQWMKLE
                     LPGGWEKDIPVFPVDPKGVATRAAGGKIMNALAPRIPFLIGGSGDLNPSTNTVLKGRG
                     NFQAPRAEDTHVQGAEAGPWSYAGANLHFGVREHAMGSILNGMALHGGILPYGSTFLI
                     FSDYMRPAIRLAALMKLRVIYVFTHDSVALGEDGPTHQPVEHIASLRAIPGLTVIRPA
                     DANEAAEAWKVAIQHEEGPVALLMTRQSIPVIDRTHFSPAEGLKRGAYVLADSPSGRA
                     EVLILASGSEVQPALAAYQKLKEEGISSRLVSMPSWELFEKQPEEYRIQVVPRESLLK
                     VSIEAGATLGWHKYVGPEGEAIGIDHFGASAPVKVILEKFGFTPENIMRRVKALLARR
                     AQR"
     gene            2510..>2716
                     /locus_tag="VEL68_02745"
     CDS             2510..>2716
                     /locus_tag="VEL68_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HXZ34918.1"
                     /translation="MKINPIKKLREINESIWLDNLSRNLINSGGLQRLIYEDGITGIT
                     SNPTIFQKAISGSKDYDLSLRRFLA"
BASE COUNT          631 a          784 c          771 g          530 t
ORIGIN      
        1 atcaccagcc gcatcggacg gacggcccga atccgggggt cccgggatta caattccttc
       61 gaggtccttc ccccccatga aatggcccgc aagaaagaac gattgcggag cggggatctt
      121 ctcttctttt ttaaggcccc gggaaaacgg gtggtcgacg aaggggtcgg ccatatggga
      181 atcgtgaagg tagagggaaa gaattcagaa aaggaaattt cgctgattca cgccggcgga
      241 accaagaact ccggaggaac cgtgagaaag gtcctgctgg tggattatct ctcggccatg
      301 ccgttcgtgg gtgtgaaggt cacgaggttt gaatgaagga aaacagtgaa agtgtatgtg
      361 aaagtgaaag gaatagattt tcacactttc actttcacta aaaaaggaat taccgatgga
      421 ccttgaccag ctttgcgtca acaccatccg catgctctcc gtggacatgg tccaggaggc
      481 caactccggg caccccgggc tgcccctggg ggccgccccc atggcctatg tcctgtggac
      541 gaaatttctc aagcacaacc cagaaaatcc gaaatggccg gaccgggacc gattcgtcct
      601 ctccgccggc catggctcag ccctcctcta ctcccttctc cacctgaccg gttatgacct
      661 gcctctcgag gagatcaagc ggttccgcca gtggggaagc aagactcccg gccacccgga
      721 gtacggagac actcccggcg tggaatgcac gaccgggccg ctcggccagg ggtttgccat
      781 gggggtgggg atggccttag ccgaacgttt tcttgccgcc cacttcaacc gcccggggca
      841 ggagatcgtc gatcattata cttacggact ggtcagcgac ggagacctca tggagggagt
      901 ggcctccgaa gcggcctccc tcgccggcca cctaaaactg ggaaaactaa tctaccttta
      961 cgaccaaaat tccatcaccc tcgcagggga aacccggctg gtctttaccg aggatgtggc
     1021 caggagattc gaagcctacg gttggcaggt tcaagcggta gaaaacggga atgacttgga
     1081 ggccattgcc cgggccatcg aaaaagcccg ggaggaaaat tcccgcccgt ccctcatctc
     1141 ggtgaggact cacatcggct atggaagccc cctcaagcac gatacctttg aggcccacgg
     1201 ttcgccccta ggcgcggaag aggttgctgc tacaaagaag aacctgggat ggcccttgga
     1261 gcccaagttc ttcattcccg aagaagtgcg ggctcatttt cataaaatca ctgcccaggg
     1321 tgcaaagatt gaagaagact gggaggcgaa gctgcggtct taccaaacgg ctttccccga
     1381 gttgcggaag cattgggacc agtggatgaa acttgaactt cccgggggat gggagaagga
     1441 tattcctgtg tttccggtgg acccaaaggg cgtggccacc cgggccgcgg ggggaaagat
     1501 catgaatgcc ctggcccctc gcatcccctt cctgatcggg ggttccggcg acctgaaccc
     1561 ttccaccaac acggtcctga agggccgagg gaattttcag gcccccaggg cggaagacac
     1621 ccacgtgcag ggcgccgagg ccggtccttg gagttatgcc ggagccaacc ttcacttcgg
     1681 ggttcgcgag cacgccatgg ggtccatcct gaacgggatg gccctccacg gcggaattct
     1741 tccctacgga agcaccttcc tgatcttctc cgactacatg cggccggcca tccgcttggc
     1801 ggccctgatg aaacttcggg tcatctacgt gttcacccat gactccgtcg ccctgggaga
     1861 ggacggcccc acccatcaac ccgtggagca catcgccagc cttcgggcca ttcccggtct
     1921 caccgtcatc cgaccagcgg acgccaatga agctgcggag gcctggaagg tcgccatcca
     1981 gcatgaagag gggccggtgg ccctactcat gacccgtcaa tcgattccgg tcatcgaccg
     2041 cacccatttc agtccggcag aagggcttaa gcggggagcc tatgttcttg ccgattcccc
     2101 ctccggccgg gcagaggtcc ttatcctggc ctccgggtcc gaggttcaac ctgcactggc
     2161 cgcctatcaa aagcttaagg aagaagggat ttcctcccgc ctggtcagca tgccctcctg
     2221 ggagcttttc gagaaacaac cggaagaata tagaattcag gtcgttccca gggagagcct
     2281 cctgaaagtt tccattgaag cgggcgccac cctggggtgg cataaatacg tcgggcccga
     2341 gggagaagcc atcggaatcg accatttcgg cgcctcggcc ccggtcaaag tcattcttga
     2401 aaagttcggg tttacccctg aaaatataat gagaagggtg aaggcccttc tcgcgcgaag
     2461 agctcaaaga taaaagtgcg atctccgatg aagaaatgag ggaaaaacta tgaaaatcaa
     2521 tcccataaag aagcttcggg aaataaacga aagcatctgg ctggataacc tgagtaggaa
     2581 tctgatcaac tccggtggac tccagcgtct gatttatgaa gacgggataa ctgggattac
     2641 ctccaatcct acgatctttc aaaaagccat ttcgggttcc aaagattacg acttatccct
     2701 gcgccgattt ctcgca
//
LOCUS       DASPRR010000077         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1629625, whole genome shotgun sequence.
ACCESSION   DASPRR010000077 DASPRR010000000
VERSION     DASPRR010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629625"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1289
                     /gene="ahbC"
                     /locus_tag="VEL68_02750"
     CDS             105..1289
                     /gene="ahbC"
                     /locus_tag="VEL68_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HXZ34919.1"
                     /translation="MIGISKLYCGTVESSDPLRYGRQSGKLPSHLLQFSEDKKPVVVW
                     NVGQRCNLKCVHCYSHSQNREYTGELSSAEGRALIEDLARFGSPVVLFSGGEPFLRKD
                     LLDLAQLATDLGMRAVISTNGTLISPDMARKLKRIGLSYVGVSLDGMEETNDRFRGVK
                     GAFQAALRGIRNCQEAGIKVGLRFTINRRNAQDIPGIFDLLEKENIPRVCFYHLVYAG
                     RGSELIKEDLDPGETRKVVDLIIDRTQNLHDQNKAIEVLTVDNHSDGPYLYLRMMGEG
                     SPRSGEVMELLRMNGGNSSGVGIGCVSWDGSVHADQFWRHYSFGNVRHRPFSQIWMDL
                     SNPLMARLKEKKRHVRGRCASCRFLEVCAGNFRVRAEAITGDLWAPDPACYLTDQEVG
                     IG"
     gene            1336..2400
                     /gene="ahbD"
                     /locus_tag="VEL68_02755"
     CDS             1336..2400
                     /gene="ahbD"
                     /locus_tag="VEL68_02755"
                     /EC_number="1.3.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HXZ34920.1"
                     /translation="MTKSILKVPPLRMIAWEVTRSCNLNCIHCRAAAQRGPYSGELNT
                     EEALRLIDEIVSFSQPVVILTGGEPLLREDIYELADYGTRRGLRMVMAPNGTLLDADK
                     AARLKTSGIQRVSISLDGATAESHDRFRQMEGAFAGALRGIEFLKEAGLEFQVNTTVT
                     LKNYHELSSLLDLAVRLGAVAHHIFLLVPTGRGKEMKGEAIEGKEYEKILHWFYDQRE
                     KVPLQLKATCAPHYYRILRQRARQEGKKVTPQTHGLDAMTRGCLGGIGFLFISHLGDV
                     QPCGYLEALSGNVRQQKLKDIWESSPVFLKLRDSSAYQGRCGRCEYMRVCGGCRARAF
                     EWSGDFMAEEPLCIYQPQCS"
BASE COUNT          718 a          787 c          837 g          608 t
ORIGIN      
        1 gaatacttca aaataagcga tcagcattca gctttcagcg gtcagcccta gaaggagctg
       61 tcagcttatc aaagagcccc ggctgaaagc taggcggttt ttctatgatc ggtatttcga
      121 aattgtactg cggaaccgtg gaatcctctg accccctgcg atacgggagg caatcgggga
      181 aactcccctc ccacctcctc cagttttctg aggataaaaa gccggtggtg gtgtggaacg
      241 taggccaaag atgtaacctg aaatgcgtcc actgctattc ccattcccaa aaccgggagt
      301 acaccggaga actatccagc gcagagggga gggcgctcat cgaggaccta gcccggttcg
      361 gttccccggt tgttctcttt tccggaggcg aacccttttt acggaaagac ctcctcgacc
      421 tggcccagct ggccacagac ctgggaatgc gtgcggtcat ctcgaccaat ggaaccctca
      481 tcagcccgga tatggcccga aaattgaaaa ggatcgggct ttcctacgtg ggagtaagcc
      541 tggatggaat ggaggagacc aacgatcggt tccggggggt gaaaggggcg ttccaagccg
      601 ctctccgggg aatccgcaat tgccaggaag cggggatcaa ggtgggactc cggtttacga
      661 tcaaccggcg caatgcccag gacattccgg gaatcttcga tcttctggag aaggaaaata
      721 tcccgagggt ctgtttttac catctggttt acgcaggccg cggaagtgag ctcattaagg
      781 aagacctcga cccgggagag acccggaaag tggtggacct catcatcgac cggacccaga
      841 acctccatga ccaaaataaa gccattgaag tgctcacggt ggacaaccac tcggatggtc
      901 cctaccttta tctgcgaatg atgggggagg ggtctccccg gtctggagag gtcatggaac
      961 tcctgcggat gaacgggggg aacagctcag gagtaggaat cggctgcgtg agttgggacg
     1021 gctcggtcca tgccgatcaa ttctggaggc attactcctt cggcaacgtg cgacatcgac
     1081 cgttcagcca gatatggatg gacttgagca atcccctgat ggccaggctg aaagagaaaa
     1141 agagacacgt aagagggcgt tgcgcttcct gccgctttct ggaagtctgc gcagggaatt
     1201 tccgggttcg agccgaagcc ataactgggg acctgtgggc tcctgacccg gcctgctacc
     1261 ttacagatca agaagttgga atcggatgat aagcaaattc cgaatcccaa gcgccaaatt
     1321 ccaaataaaa tgacaatgac caaaagtatc ctgaaggttc ctcctctgcg gatgatcgcc
     1381 tgggaagtca cccgaagctg caatctcaat tgcatccatt gccgggcggc agcgcagcga
     1441 ggcccctact ctggggaact gaataccgag gaagccctcc gcctgattga tgagatcgtc
     1501 tccttcagtc agcccgtagt tatccttacc ggcggggagc cgctcctccg cgaggatatc
     1561 tatgagctgg ccgactatgg gacccgtcgg gggctgcgca tggtcatggc tcccaacggg
     1621 accctgctgg atgcggacaa agccgcccgc ttgaaaacca gtggaatcca gcgggtctcc
     1681 atcagcctcg acggagcaac ggcggaaagc catgaccggt ttcgccagat ggaaggggcc
     1741 tttgccgggg ccctgcgggg gatcgaattt ctgaaagagg ccgggctcga attccaggtc
     1801 aacaccacgg tcaccctgaa aaattaccac gaattgtcct ccctattgga cctagccgtg
     1861 agactggggg ctgtggctca ccacattttc ctcctggtcc ccacgggaag ggggaaggag
     1921 atgaaagggg aggccatcga ggggaaggag tacgaaaaaa tccttcactg gttttacgac
     1981 caacgggaaa aagtgcctct tcagttgaag gcgacctgtg ccccccacta ctaccggatt
     2041 ttgcggcaaa gagccaggca agagggaaag aaagtaaccc cccagactca tggccttgac
     2101 gctatgaccc ggggatgctt gggagggatc ggattcttat tcatatctca tctgggggat
     2161 gtgcagcctt gcggctacct ggaggcactc agcggaaacg tgagacagca gaaattgaaa
     2221 gacatatggg aatcctcccc ggtgttcttg aagttacgcg attcctccgc ttatcaaggt
     2281 cgttgcgggc gctgcgaata catgagagtc tgtggagggt gccgcgccag ggccttcgaa
     2341 tggtccgggg actttatggc cgaagaaccc ctctgcattt accaacccca atgttcctga
     2401 aggtttcgat tactggcagg cccggaaatt acccaaccca gggcagccct ttccgtcctg
     2461 aaatttcccc tccgagaata tcaccgctgg actccatcga agcaagagcc atagtccctt
     2521 tcagaccatc attttttgga cagagcccat tggcgatttg tacaattatg accggaattg
     2581 gggggggttc gatccgcctg gtttagggcc ataaggaaga ataaccctgc aaaatgattt
     2641 gcctcaattc gaaaatcagc tgtgttataa aagggttcgg cgatgaatcc atcccaaaag
     2701 cagtaggatt cgggtttatc gatctgatga tttgcctgcg ggcacaaaaa ttgcggcatc
     2761 gaaaatgccg ctggagggat ctcttcttcc ttcagaatct aaggcgagca aggataccga
     2821 aaatgcctca gctattgaat tctgtccaga gcgagcccga gattctcccg ctgaagccgg
     2881 tggaagcgat ccttccagtc ccgccccccc caaaacctcc ggataagaga atcgcgcgga
     2941 aaaagtcagg
//
LOCUS       DASPRR010000078         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1794710, whole genome shotgun sequence.
ACCESSION   DASPRR010000078 DASPRR010000000
VERSION     DASPRR010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794710"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1167
                     /locus_tag="VEL68_02760"
     CDS             352..1167
                     /locus_tag="VEL68_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ34921.1"
                     /translation="MTHGNKVLLEIQSAIALITLNKPDRLNALDPGVWMELRSAAEAV
                     EANPAVRAAILTGAGDRAFCAGLDLKEGSTFTLEDGYSPAATLPKIHEHLQRLRSSFD
                     AIENLRVPVIAAIQGHCIGGGTELICCCDIRIGSDDSRYSIPEVKLGIVPDMGGTQRL
                     PRIIGIGKAKELIYSGRSIDAQEALRVGLLNEVCPKDKVMERAYLLAREIVANAPLAV
                     QAAKKAINGGWSQTLPVGLAMENSRAAEVLLSEDARGIRSAGVQKKELIFKGR"
     gene            complement(1204..3522)
                     /locus_tag="VEL68_02765"
     CDS             complement(1204..3522)
                     /locus_tag="VEL68_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34922.1"
                     /translation="MANFPLHFALLTMGFTFTISQVMVIRELLVVFAGNELSIAIILA
                     NWLLLEAIGSFFVGRKVGEWGLREGGYAFFQILLSALLPLTLYVIRSWNDFMGLAPGE
                     GASLLQILFWTIPVLAPVGILDGILFALGCTLNSKWTEKGAFSIGRVYLLEAIGAGSG
                     GVLYTFLFIPYLGSFQVASILGITNLLSGFLLVAKCGGVHKWKWAFLGLWGYFAFVNL
                     LLLIPYSSQTVEKISMQKLWRGFEILDSRWSPFGNVTVARREEQLTFFSNGIPLCNVP
                     VPNISFVEELVHYPLLLSPSPKKVLIVGGGFGGVIREILKHPVEEVHYAEIDPLIIQL
                     IRENQTPLTRQELENPKVRVHILDGRHFIRTTRQVFDTILLNLPGPFTLELNRFYTVE
                     FFQEAFRLLGMDGILALPVPGSETYLSPEARELNLSILKSLRETFSSVVIIPGDPNFI
                     LASPSEKLEPLTADRLARSLQKNKIATQFVTPALIKRKMEKQRMEWLDEALKQGEEVR
                     MNRDLNPSGIYYGIAYWNAQFHPSVQALWNRMGEFRLSYFGLVLLGLVGAVAVYRKRL
                     RAGEGKRALRGTLIWVVTTSGFFGMAMSILLILSFQTLYGYAYGWIGLLVAAFMAGLA
                     LGTWMINRDLERSQNWIMTLVGLEGFIALFCLMVILILYFLYSSGKEPLNLWTEKIGF
                     LLMSFLSGFWVGLEFPLSSMIFVGNEKGVARTAGILYAADLLGAWAGALLVGVIFIPV
                     LGILLTCGGIILLKLASLALLGIAEFGFWNAE"
     gene            3502..>3900
                     /gene="recO"
                     /locus_tag="VEL68_02770"
     CDS             3502..>3900
                     /gene="recO"
                     /locus_tag="VEL68_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXZ34923.1"
                     /translation="MKREVGQSDLPEEGISCKGNYSTPPPSHPLSFLLRFLMPIYKTP
                     AVIFHTLPYGESDQIVTFYTLDFGKVKGIAKGAKRSRKRFGVRLEIASYVKVTFFEKE
                     TSSLVRLSNCDLIRSFEGLREDIKKLAWASY"
BASE COUNT         1001 a         1088 c         1041 g          770 t
ORIGIN      
        1 gccgtcctcc acctcgtgct tcccaaagaa atgcagccaa cccaacctag ccagggattg
       61 ccggtgcagg gagccccccc cccgaaagat ttcaaaccgg cccctcccat ttcttctctt
      121 ccatcatcca aggaagagcc cgtgaaagaa accaggtcct ctgcaggcga tgtttccgag
      181 aaaagcaggc cattactgga gacctccccg aaagaaacca agcccccgga aagcgttcct
      241 gctcaggaaa acaaaacatc cgatgaagac caagaggcta tcaagaacct aagatgatca
      301 acggatgggc gggagggatg ggaagaagac ggagggtacg gagaggaagc tatgactcac
      361 gggaacaaag ttcttctcga aattcagagc gcgatcgccc tgatcactct gaataagccg
      421 gaccgcctga atgccctgga cccgggggtt tggatggagt tgaggtccgc ggccgaagcg
      481 gttgaggcga acccggccgt tcgagcagcc attctcactg gggccgggga ccgggctttc
      541 tgcgccgggt tggacttgaa agagggaagc acgtttaccc tggaggacgg ttattccccc
      601 gcagccaccc tgcccaaaat ccacgagcac cttcagcggc tccgttcttc ctttgatgcg
      661 atcgagaacc tgcgcgttcc ggtgattgcc gcgatccagg ggcattgcat tggaggcggg
      721 accgagttga tctgttgctg cgacattcgc atcggctccg atgactcccg ttattcaatc
      781 cccgaggtaa agctggggat tgttccggac atgggtggga cccagcggct gcccagaatc
      841 atcggcatcg gcaaggccaa agaactgatc tattcgggtc ggtcaatcga tgcccaggaa
      901 gccctccggg ttggactcct caacgaagtc tgccccaagg ataaggtgat ggaaagggct
      961 taccttctgg cccgggaaat cgttgccaac gctcccctgg ccgtccaggc ggccaagaaa
     1021 gccatcaacg gcggctggag ccagaccctc ccggtgggtt tggccatgga aaactcccgg
     1081 gctgccgaag tccttctctc cgaagacgcc cggggaattc ggtcggcagg ggtccagaaa
     1141 aaggaattga tctttaaggg acgatagaaa ctggatcaaa gagacagtcg atttcccgtt
     1201 tttctattcc gcattccaaa atccgaattc cgcaattccc aagagggcca ggctggccag
     1261 tttcagaagg atgatccccc cgcaggtcag gagaatcccc aggacgggaa tgaagatcac
     1321 gcccaccaga agggcgccgg cccaggctcc caaaaggtca gcggcgtaga gaatcccggc
     1381 cgtccgggcc acccccttct cgttgccgac gaaaatcatg ctggataaag gaaattccag
     1441 gccgacccaa aatccggaaa ggaaactcat cagaaggaag ccaatcttct cggtccacag
     1501 atttagaggc tccttccccg atgaatacag gaaatacaga ataagtatca ccatgagaca
     1561 aaaaagagcg atgaaaccct ctaagccgac cagagtcatg atccaattct gactcctttc
     1621 caggtcccgg tttatcatcc aggttcccag ggccagtccg gccatgaagg ccgcaaccag
     1681 aagcccgatc catccgtaag catatccata aagagtctgg aaggacagaa tcaaaagaat
     1741 gctcatggcc attccgaaga accccgaagt cgttaccacc caaatgagtg tccctcgtag
     1801 agcccttttt ccttcccccg ctcttagcct tttccgatag accgcaactg cccccaccaa
     1861 tcccaagaga accagtccga aataggatag gcgaaattct cccatccggt tccacaaagc
     1921 ctggaccgag ggatggaact gggcgttcca gtaggcaatt ccatagtaaa ttcccgaggg
     1981 atttaggtcc cggttcatac ggacctcctc cccctgcttt agcgcctcat ccagccactc
     2041 catacgctgc ttttccatct tgcgctttat gagggcagga gtcacaaatt gggttgcgat
     2101 tttattcttc tgaagggaac gggcaagacg gtcggccgtg aggggctcta gtttctctga
     2161 gggggaggcc aggataaaat tcgggtctcc gggtatgatc accaccgaag agaaggtttc
     2221 ccgaagagat tttaggatgc tcaggttgag ctcccttgcc tcgggtgaaa gataggtttc
     2281 agaaccggga acaggaaggg ccaggattcc gtccatcccc agcagccgaa aggcttcctg
     2341 aaaaaattcg accgtataga accggttcag ctccagggta aagggaccgg gaaggttgag
     2401 gagaatagta tcgaatactt gccgggtggt tctgataaaa tgccgcccat ccagaatatg
     2461 aacccggacc tttgggtttt ccagctcctg gcgggtcagc ggagtctgat tttcgcggat
     2521 caattggatg atcaggggat caatctccgc ataatgaacc tcttccacgg gatgcttgag
     2581 gatttctcga atgaccccgc cgaaaccccc tcccacgatc aggaccttct tgggcgaggg
     2641 gctgaggagc agaggataat ggaccagttc ctctacaaaa ctgatgttgg ggaccgggac
     2701 attgcacagg gggatgccgt tggagaagaa agtaagctgc tcttcccttc gggccacggt
     2761 tacattccca aagggagacc agcgggagtc caaaatctca aatccccgcc acaacttttg
     2821 catggagatt ttctcgacgg tctggctgct ataggggatg aggaggagca ggttgacaaa
     2881 tgcaaaatat ccccataacc ccaggaaggc ccatttccac ttgtggaccc ctccacactt
     2941 agcaaccaaa aggaatccgg ataggagatt cgtaatgccc aggatggagg ccacttgaaa
     3001 agaccctaaa taagggatga acaagaaagt gtagagcacc cccccacttc ctgccccaat
     3061 cgcttccagg agataaaccc ggcctatgga gaaggctccc ttttcagtcc attttgagtt
     3121 aagggtgcac cccagggcaa agagaattcc atccaggatt ccaaccggag ccaggacggg
     3181 aatcgtccag aaaagaatct gcaagaggct ggctccttct cccggagcaa ggcccatgaa
     3241 atcattccag gagcggatga cgtaaagggt cagggggagg agggccgaca ggaggatctg
     3301 aaaaaaggca tatccccctt ctcgaaggcc ccattcccca acttttctcc cgacgaagaa
     3361 actgccgatc gcctccaaga gaagccagtt ggccagaatg atggcgatgg acaactcgtt
     3421 tccggcgaag accaccagca gctcccggat aaccatcacc tgggagatgg taaaagtaaa
     3481 ccccatggta aggagggcga aatgaagagg gaagttggcc aatccgatct ccccgaagaa
     3541 ggtatatcct gcaagggaaa ttatagcacg cctcctcctt cccatcccct ttcctttctg
     3601 ttaagatttc taatgcccat ctacaaaact ccggcggtta ttttccacac tttgccctac
     3661 ggcgagtcgg atcagatcgt caccttttac accctcgact tcgggaaggt gaagggaatc
     3721 gccaagggag ccaagcggtc gcgaaagcga tttggggttc gcctggagat cgcctcctat
     3781 gtgaaggtta ctttttttga aaaagagact tccagcctcg tccgcctgag caactgcgac
     3841 ctgatccgct ccttcgaggg tctcagggaa gatatcaaaa aactggcctg ggccagctat
//
LOCUS       DASPRR010000079         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_335149, whole genome shotgun sequence.
ACCESSION   DASPRR010000079 DASPRR010000000
VERSION     DASPRR010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335149"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(489..2201)
                     /locus_tag="VEL68_02775"
     CDS             complement(489..2201)
                     /locus_tag="VEL68_02775"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HXZ34924.1"
                     /translation="MDLFVIHGIVEELKKEITGTFISKVYQMNRTDLLLRLRRSGDEK
                     QLLLSTHPDFFRLHLTEKKYANPMVPPRFCTYLRKHIGGARVSGVTQDRYERVVRVGL
                     QKMMDAGVIREVFLVAELVGKMSNLLLLEGDKILDCLHFRRGEEGAPRPSAPGLFYVP
                     LSPSSQWAPDEMDQEKMAEISSSPVGERWRAMVHRISGVSPLLARELEFVSDGTPEGI
                     GEKLRLLTQRYEKADFEPRVYTLLGEKKVLSPFPLKSLGPAEEETFPSMNQAADQFYF
                     EVVMKRQMIEQKQSLSKRLRQLISRLQRRQDNLTTDREGFQKDLEAKDYGDILTANYP
                     RIKRGMREAEVLDYRQDPPRSILIPLDESLDPSANVQRYFKKYKKAKRGLEIVSQRVE
                     ETEKEIVYLESVLFGVEAAEDAEEVEMIRRELEEERIIPVSKKQKAGKEEKEKSLPVR
                     RFRSSEGLEILCGKHNLGNDYLLRHLAKDNDLWFHSQGLPGSHVLLKTGKGDPKPESI
                     LEAATIAAHYSRGSGSTRLPVDYTPVKNVHRPKGAKPGMVTYFHQKTIQVRPDKEKVE
                     RLRT"
     gene            complement(2606..3157)
                     /locus_tag="VEL68_02780"
     CDS             complement(2606..3157)
                     /locus_tag="VEL68_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34925.1"
                     /translation="MARRPSPEIKGFSDSFFGKDQEAREFEPGDFILTKSSGILGKLI
                     RTGQLIRFKGKYAKWTHAALIVSKDGSLIEASGRGVVHSHISAYKKKDYHLIRLGEMA
                     DQRDRQKMVNFAEWCLRYDYGHLTIISISLCLITGWKFLFGMDNRIICSALVARALER
                     TRVIFDKNPSFIMPADLAKYFRI"
     gene            3421..4683
                     /locus_tag="VEL68_02785"
     CDS             3421..4683
                     /locus_tag="VEL68_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ34926.1"
                     /translation="MEQKEIHQQRVFYGWIITGLVFLNLGTAYGAQYSFGVLFPAMIE
                     EFKWNRQNLAGAFSLYTFLYGSLGIILGRWVDRFGPRVVLIAGSICLGMGIGLISQVR
                     APWHLYLVYGFLAAWGMSAAYMTASPTVVKWFVEKRGLALGIAQSGLGIGIVLIPLLV
                     GTLISSFGWRHACMILGALVFAVLFSTSLFMVGHPEKVGLLPDGRRVHEPDSPQKTSL
                     ESISKEVYWSATEAMHTRSFWVLTGIFFFTWLLVFLPLVHLVIFARDIGLSQKSALFA
                     LSFLGGSSTIGRLTMGYVSDRIGRKRTLGLNLALQVLSWLWILATHNAWMLIVFAVFF
                     GFSYGGVSAVFPSIVGDYFGRLKAASVIGAIFAISGVSSAFGPFVGGYIYDLTHTYNF
                     AFLLGGLTNLLALLLLLLSKPPTKTVRS"
     gene            complement(4680..5621)
                     /locus_tag="VEL68_02790"
     CDS             complement(4680..5621)
                     /locus_tag="VEL68_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXZ34927.1"
                     /translation="MKTRCALLLNNKTSPASHWIADAVLLGVSVFWGTSFAIIKEALG
                     ETTPANLLFIRFFFSCLLLLPVGWTRRKTYSREFLKPGVIIGFFLFTAFFTQAWGLVF
                     TTASRSGFITGLSVILVPLLAILVLRQMPARSAMVGAALAFCGLYLLTTVDQTQALSF
                     NWGDFLTLICAFFWAGHILALGRYSPGGDTFWLTFIQLATSCIGSLGWVVLTGDLNLR
                     LPTGVYGAAFYLALTCTVLAYLGQTWAQARTTATRTAIILSMEPVFAAFFAWFWLGEK
                     VGVWGWMGAASILAGILLAELGPFLTRRPENSGTPTS"
BASE COUNT         1388 a         1653 c         1617 g         1483 t
ORIGIN      
        1 gtcccgaagt tctatgaaga acctggagcc cacttcgccc accgaccaag tgtagggaac
       61 cttgatcctt ccctgataga ccaagtcttc ctctggttca ttccacttgt tcataaccct
      121 ctccttagaa gttgtccgtc aagctgcgcg gcaaaacaaa gcggagtccg ctcacgccga
      181 gccgggaggt ctaaatcgga tatttcccgg ggaatccaga tagaagatga ttaccggtga
      241 ttcttccgcg gcaggattca attccagaag gggcagtctc ttcatgggtt tccctccttg
      301 gctgaatcag gactcgattg aggaacaaag ggcaggaaaa ctcaccgttc ttatagcaaa
      361 gtagagaagg gaagtcaaac ccaaaaaggc tctggcgcac caaaattttc aaaaaagcgg
      421 gaaaggggtc cgagaatgag ttcaggttct aaaagctgaa acaacccaaa ggacggctag
      481 aaaaaatctt aagttcttag cctttccact ttttccttat ccggcctcac ctgaattgtt
      541 ttttgatgaa aatacgtcac catcccgggc ttggcccctt tggggcggtg cacgttcttc
      601 accggagtat agtccaccgg gagccgggtg gaaccgctcc cccggctgta atgcgcggca
      661 atcgtggccg cttcgagaat tgactccggt ttggggtccc ccttcccggt tttcaagaga
      721 acatgggaac cgggaagtcc ctgggaatgg aaccacaggt cgttatcttt ggccagatgc
      781 cggaggagat agtcgttccc cagattgtgt ttcccgcaga gaatctccaa tccctcggaa
      841 gaacggaagc gacgcacggg aagggatttt tctttttctt ccttcccggc cttctgtttc
      901 ttcgagacag ggatgatcct ttcctcttcc agttcccgac gaatcatttc tacttcttcc
      961 gcatcctcgg cggcttcgac tccgaacaga accgattcca gataaacgat ctccttctcc
     1021 gtttcttcca cccgttggga gactatttcc aaacctcgct tggctttttt gtacttcttg
     1081 aaatatctct gcacattggc tgaagggtcc agggattcat ccagggggat caatatcgac
     1141 cggggcgggt cctgccggta atccagaact tccgcttcgc gcatccccct cttgatcctg
     1201 ggataattgg cggtcaaaat gtctccatag tccttggcct ccaggtcttt ttgaaatcct
     1261 tcccgatcgg ttgtcaaatt atcctgccgt ctctggagcc gggagatcag ctgccgcagc
     1321 cttttggaaa gggactgctt ctgctcgatc atctggcgct tcatcactac ttcgaagtag
     1381 aactgatcgg cagcctgatt catggaaggg aaggtctcct cctctgccgg ccccaggctc
     1441 ttcaggggga aaggagacaa taccttcttt tcccccagca gcgtatagac cctcggctcg
     1501 aaatcggctt tctcgtatct ttgcgtcagg aggcgtagtt tttcgcctat cccctcggga
     1561 gttccgtcgc tcacaaactc caattcccgg gccagaagcg ggcttacccc ggagattctg
     1621 tgaaccatgg ctctccagcg ctccccgaca ggagaggaag aaatttccgc catcttctcc
     1681 tgatccattt catccggagc ccattgacta gatggagaaa ggggcacata gaaaaggccg
     1741 ggggccgagg ggcggggcgc tccttcttca ccccggcgga aatgtagaca atcgaggatt
     1801 ttatccccct ccagcaaaag gagattgctc atttttccca ctagctcggc gacgagaaag
     1861 acctcccgga tgactcccgc atccatcatc ttctggaggc caacgcggac gactctttca
     1921 taacgatcct gggtgacacc ggagaccctg gccccgccaa tatgctttcg caggtacgtg
     1981 caaaagcgcg ggggaaccat cggattggca tacttctttt ccgtcaagtg gagacggaag
     2041 aaatcagggt gggtggaaag gagaagctgt ttttcatcgc ccgagcggcg aaggcggagg
     2101 agaaggtcgg tccggttcat ctggtagacc ttggagatga aagtccccgt gatctccttc
     2161 tttaattcct cgacgatgcc atggataacg aagaggtcca tatctgggga ggattgggct
     2221 gggcctggtt agtgtcctga gtcctgaatt cggctcctaa ttttcttaag gtatccctcc
     2281 gctctgccgg gggtccgcct ggggcggatt gacatttccg gaaagcttgg gtcgtggccc
     2341 ggggacaagg gtgttgccca cagaaagcat cggccttcat attgcggatt tcgcattgtg
     2401 gattccggat ttttgggggc ctttcttttt caatccgcat tccgcaatct acaatctgca
     2461 attgcgacgg cccgcccgtt ccatggaatc aaaggcgcct catggtttct gtgggcaacg
     2521 cctttgcccc cgggccgggc aatggttcgc tgagccacgg ataaagcatg aaaaggaatt
     2581 ctttttcgga gcgatcgatg gtttttcaaa tccggaaata tttagccaaa tcagcaggca
     2641 tgatgaagct gggatttttg tcaaaaataa cccgggtccg ctccagggcc ctggctacca
     2701 aggcggagca gatgattcgg ttgtccatcc cgaacaggaa cttccacccg gtaatcaggc
     2761 acaaagaaat cgagataatc gtcaagtggc cgtaatcata ccggagacac cactcggcga
     2821 aattcaccat tttctgccgg tctctctggt cggccatttc acccagacgg atcaggtggt
     2881 agtccttctt cttgtatgcg gaaatgtggg aatggaccac tcccctcccg ctggcctcga
     2941 tcagacttcc atctttgctc acaatcaggg cggcatgagt ccacttggca tacttcccct
     3001 tgaagcggat caactggccg gtccggatca gcttgcctag gatccccgag cttttggtga
     3061 gaataaagtc gcccggctca aattcccggg cctcctggtc ttttccaaag aaagaatcgc
     3121 taaagccctt gatttcgggg ctgggtctcc gggccataat ttggcgctca ctttgaacag
     3181 gttttgcccc ttattaaaac acattctccc ttcttcatca atccactctt ttcttgccag
     3241 atagtggtct ttgcgataac atcccaggaa aaaattcaaa atccgaagca ccaatatcga
     3301 aacaaattga aatgacctga agttcaaggc agcccttttt gagcatggaa gattcggatt
     3361 tgtttcgtcc cacatttcgt ggaattcgat attcgagttt cggatttctt aaaattattt
     3421 atggaacaga aagaaatcca tcagcaaaga gtattttacg gttggattat taccggtctg
     3481 gtgtttttga acctgggcac ggcttatggg gcccagtatt ccttcggggt tctgtttcct
     3541 gccatgatcg aagaattcaa gtggaaccgg cagaatctgg ctggggcctt ttccctctac
     3601 actttcctct acggttccct ggggatcatc ctgggccgat gggttgaccg cttcggccca
     3661 agggtcgtct taattgctgg cagtatttgt ctggggatgg ggatcggctt gatcagccag
     3721 gtccgggccc cctggcatct ctatctcgtc tacggattcc ttgccgcctg gggaatgagc
     3781 gcggcctaca tgaccgccag ccccactgtc gtaaaatggt tcgtcgagaa gcggggactg
     3841 gccttgggaa tcgcccaatc cgggttgggg attggaatcg tgctcatccc gctcctcgtg
     3901 ggaaccctga tttcctcttt tggctggcgg cacgcctgta tgatcctggg agccttggtc
     3961 ttcgccgttc tcttctccac ttctctgttc atggtcggcc acccggaaaa ggtcggactt
     4021 ctgcctgacg gtcgaagggt ccacgaaccc gacagccccc aaaagaccag cctggagagt
     4081 atctccaaag aagtgtactg gtcggcaacg gaagccatgc acacccgttc cttttgggtg
     4141 ttgacaggga tcttcttctt cacctggctt ctggtcttcc tccccctggt tcacctggtc
     4201 atttttgccc gggatatcgg cctctcccag aaatcagccc tattcgccct gagctttctg
     4261 ggcgggtctt ctaccatcgg gcgtttgacg atgggctacg tttcagaccg aatcgggagg
     4321 aagcggaccc tgggtttgaa cctcgccctc caggttttat cctggctctg gatcctggcg
     4381 acccataacg cgtggatgct gatcgtcttc gccgtctttt ttggtttttc ttacggagga
     4441 gtcagtgcgg tctttccgtc catcgtggga gattattttg gaaggctcaa ggccgcttcg
     4501 gtgatcggcg ccatttttgc catttcggga gtctcgagcg ctttcgggcc tttcgtcggg
     4561 ggttacattt atgatttgac tcacacttat aatttcgctt ttcttctggg agggctcacc
     4621 aatctcttag cccttttgct cttgttgctc tccaagccac cgacaaaaac ggttcgaagt
     4681 taggaggtcg gagttccgga attttcgggt cttcttgtaa ggaaagggcc caattcggcc
     4741 aggagaattc cggccaggat cgaagctgcg cccatccatc cccacactcc cactttttcc
     4801 cccagccaaa accaggcgaa gaaagcggcg aaaaccggct ccatggacag gataatcgcc
     4861 gtgcgggttg ccgtggtcct tgcctgggcc caggtctgcc ccaggtaggc cagaaccgtg
     4921 caagtcaggg ccaggtaaaa ggcggcccca tagactcccg ttggcaagcg aagatttaaa
     4981 tcgccggtaa ggacgaccca acccagactg ccgatgcacg aggtcgccag ctgaatgaag
     5041 gttagccaga aggtatcccc cccgggggaa tatcgaccca gggccagaat gtggccagcc
     5101 caaaaaaagg cacagatgag ggtgagaaaa tctccccagt taaaggaaag ggcctgagtc
     5161 tggtcgacgg tggtcaggag gtaaaggccg caaaaagcca gcgctgctcc caccatggca
     5221 gaccgggcgg gcatctgcct caaaaccaaa atcgccagca aagggaccaa aataacgctc
     5281 aagccggtga tgaacccgct ccgggaagcg gtggtgaaga ccagccccca ggcctgagtg
     5341 aagaaggcgg tgaaaagaaa aaagccgatg atgactccgg gtttcaagaa ttcccgggaa
     5401 taggtttttc tccgggtcca tcccacgggc aggagtaaca ggcaggaaaa aaagaaacgg
     5461 atgaaaagga gatttgcagg agtggtttcc cccagcgcct ccttgatgat ggcgaaggag
     5521 gttccccaga aaacggatac cccgagcaaa accgcatcgg caatccaatg agaagcggga
     5581 gaagtcttgt tgtttagcaa aagtgcgcac cgggttttca aaatgaacct cattgtacca
     5641 atggcccgat ttcccgatca accagaatcg acaaagcttg cccaccccca gttcttaaaa
     5701 aagagtcctt cgaaaataag atctctggaa aggagcgggg atctcatggg gttcagggcg
     5761 gaggaatttg gatcatcatc ctgaggcggt ggttcctcca tgggatcggg cctgtcctgg
     5821 ggagggttga aatggaaatg ttttggctct cttttctgct tggaggaatc tcctcccagg
     5881 cctctccctg cggaacatgg gggggagttt catagaagga taagcacccg ccgagcctga
     5941 tgatccaaat tcctccaccc ggaccccaac aagcacgaaa aactgggggt gggcaaggaa
     6001 tcgacaaagc agttgactct cttcaaaccc tatgctatcc caataaaaaa atcagggcgc
     6061 agataatcga gatagatatt tgttgaatca tgagcccaga ttatttttat agtcaccgac
     6121 ttaaaaagtt gacgttgggc g
//
LOCUS       DASPRR010000080         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_335152, whole genome shotgun sequence.
ACCESSION   DASPRR010000080 DASPRR010000000
VERSION     DASPRR010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335152"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VEL68_02795"
     CDS             <1..702
                     /locus_tag="VEL68_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34928.1"
                     /translation="EEAVEEGIKIQNGWGPRKILQHEQGSLRLELSRCLRIFDEQGRF
                     SPQLDDFRGLSPSAESVIVAIGQQPDLSLLPPEIRPSPRSNLLVDPLTLQSRREKIFA
                     AGDLTSGARSVIEAMAQGREAAVSIDRFLQGETLRWGRAYWDGTCITDFPVDRSGTIV
                     RPRASLPRLPVQSRNALNEIEKALGTKEAREEAERCLNCGRPGEVNQTCWYCLPCEIE
                     CPVDALEVRLPYLVR"
     gene            complement(794..1825)
                     /locus_tag="VEL68_02800"
     CDS             complement(794..1825)
                     /locus_tag="VEL68_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ34929.1"
                     /translation="MSPQKQKLGVGIRGAGQVSYEHAKAIGHNPRLYLAAVCSRSLES
                     AQKLAREFNPEAKVYAHYEDLLADSSVDVVSICMPNYLHAKEAIFALHAKKHLILEKP
                     TAVNREELAALRQAARKAETRTVVSFVARWHPMIKNLRSLLDRKALGHIYYVEADYWH
                     GIKSTFASYPWIRRKEFAGGAMITGGCHAADLARYLKGEEIVEVSAYRCRNREDFDYF
                     TTLVAAVKFRDGAVGKLSASLDGLLFPYQLNIDLLGTEGAIRDNRVYSKGLFPVQTDW
                     ITLSTPTPNSGAVTHHPFQEEIDNLVANIYEDVPVLSDVLDACNSMEVAISIEESAAT
                     EKPVTVTGQ"
     gene            2250..3080
                     /locus_tag="VEL68_02805"
     CDS             2250..3080
                     /locus_tag="VEL68_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXZ34930.1"
                     /translation="MIKISSMVGTPDLKAPVLAPYKGDLPVAFDKLAKMGYDGVEFMT
                     KNPSKLNGVAIRRSLDDYNLELCQICTGHVWGEDKLGLVNHDATLNREAMKRMKEIVD
                     FAAAYFKPGVPVNIGRSRGQGDPSQPEKSLAAYEAAFQELADYAAPTKVRLTLEPITA
                     TLIKFVLTTQDGIDMCRRVNRPNFGLMLDVYHMNIEDVDIYQSFRDARDYTWLVHVCD
                     NNRKWPGSAHLDFAQIIGTLNEIRYDGFVSTEMLPWPDPDTSARAAIEHLRRFIPKPK
                     "
BASE COUNT          913 a         1063 c         1133 g          864 t
ORIGIN      
        1 gaagaggctg tggaggaggg catcaagatt cagaacggct gggggcctcg aaaaatcctg
       61 cagcatgaac agggcagcct gcggctggag ttatcccgct gccttcggat tttcgacgag
      121 cagggccggt tctctcccca attggatgat ttccgcggac tgtctccttc ggctgaatcg
      181 gtcattgtgg cgatcgggca gcagccggac ctttcattgc tgcccccgga gattcggcct
      241 tcccctcgat caaacctgct ggttgacccg ttgactttgc agagccgcag ggaaaaaatc
      301 ttcgccgccg gggatttgac ctccggtgcc cgttcggtca tcgaagccat ggcccagggg
      361 agggaagcgg cagtctccat cgaccgtttc ctccagggag aaacgctgag gtgggggcga
      421 gcctactggg acggtacctg catcaccgac tttcccgtcg atcgatccgg aaccatcgtg
      481 cgccccaggg cctcacttcc ccggcttccg gttcaatccc gcaatgcctt gaacgagata
      541 gaaaaagcat tgggaacgaa ggaagcccgc gaagaagccg aacggtgcct gaactgcggc
      601 cggccggggg aagtcaacca aacttgctgg tattgcctcc cgtgcgagat cgaatgtccg
      661 gtggatgcac tagaggtccg tttgccgtac ttagtaagat aagcctccct ccggctcaat
      721 tgaaggagtg aggacagaat tcagcggaac ggagggtgat aagagaattt gcctcctgca
      781 ttgattccgc attttattgg ccggttacgg taacaggttt ttccgtcgct gccgactcct
      841 cgatggagat agccacctcc atggagttgc aggcgtccag gacgtcggaa aggaccggta
      901 catcctcata gatgttggcg accaggttgt caatctcttc ctggaaaggg tgatgagtaa
      961 ccgcacccga gttgggagtc ggggtagaaa gggtgatcca atccgtctga acggggaaaa
     1021 ggcccttgga atacacgcgg ttgtcccgaa tggcgccttc ggtcccgaga aggtcgatat
     1081 tcagctggta gggaaaaagg aggccgtcga gagaagccga gagcttaccc accgcgccat
     1141 cccgaaactt cactgccgcc accagagtgg tgaaataatc gaagtcctcc cggtttcggc
     1201 aacggtaggc ggacacctct acgatttctt ctcctttcag ataccgcgcc aggtcggcag
     1261 cgtggcatcc cccggtgatc atggccccgc cggcgaactc cttgcgccgg atccaggggt
     1321 agctggcaaa ggttgacttg atcccgtgcc agtaatcggc ttcgacgtaa taaatgtgcc
     1381 caagggcttt tcggtcaaga agggaacgga ggttcttgat catgggatgc cagcgggcca
     1441 cgaaactgac cacggtgcgg gtttccgctt ttcgggccgc ctgacgcaag gctgcgagct
     1501 cttcgcggtt gacggccgtc ggcttctcca ggatcaggtg tttcttcgcg tggagggcga
     1561 agatggcttc cttcgcgtgg aggtaattgg gcatgcatat ggaaactacg tccaccgagg
     1621 agtcggcgag gaggtcttca taatgggcgt acactttcgc ttcggggttg aactcccggg
     1681 ccagtttttg ggcgctttcc agggatcggc tgcacacggc ggccaggtag aggcggggat
     1741 tatggccaat ggccttggca tgctcatagg aaacttgacc ggctccccgg attcccaccc
     1801 ccaatttttg tttctgcgga ctcatcacga tctccccaaa gaattattga aatacaggaa
     1861 aacaaaattg aacacaggcg aggaaatgca aaaacccccg aaggcgatat tggatgcgaa
     1921 tgaacgcgga tagaaactca taagatggga ataaatggat ttcaaaaaat atcaggatgc
     1981 acttcccgaa cgcaccgttt aaaggcttga acacaagtgt atcatatccc agaggttcct
     2041 tcctcgtttt tcattgaccc atggagacat ggcccgctat actcaaacta atccgacatg
     2101 attaggatat tttcccatca cgccttgccg taatgggacg gaatggaaga ggagcggcat
     2161 ggtgtaccag gatcggccga gaatccctcc gtatgtaaaa tcctcctggg cacctttatc
     2221 agggtatttt gtttccagga gataggaaca tgatcaaaat ttcctccatg gttggaaccc
     2281 cggacttgaa agcgccagtc ttggcgcctt acaaaggtga tctgcctgtg gccttcgaca
     2341 agctggcgaa gatgggctac gacggcgttg agttcatgac caaaaaccca tcgaagctaa
     2401 acggggttgc catccgtcgt tcgctggacg attacaacct ggaactctgc cagatatgca
     2461 ccgggcacgt ctggggggaa gacaaactgg ggctggtcaa ccacgatgcc actttgaacc
     2521 gtgaggccat gaagcggatg aaggaaatcg tagactttgc cgctgcatat ttcaaaccgg
     2581 gggtcccggt gaatattggc cgctcccgcg gccagggtga tccctcgcag ccggagaaga
     2641 gcctggcagc ctatgaggcc gcgttccagg aactggctga ttatgcggca cccaccaaag
     2701 tgcgtctgac gctggaaccg atcacggcca ccctgatcaa attcgtcctg actacccagg
     2761 acgggataga tatgtgccgg agggtaaacc gccccaactt tggtttgatg ctggacgtct
     2821 atcatatgaa tattgaagac gtggacatct accagagttt ccgcgacgcc agggactata
     2881 cctggctcgt ccatgtgtgt gacaacaacc gcaagtggcc aggaagcgcc cacctggatt
     2941 ttgcccagat catcggtacc cttaacgaga tcaggtatga cggattcgta tccacggaga
     3001 tgcttccctg gccggacccg gacacttcag cccgcgcggc catcgaacac ctgcggcgtt
     3061 ttattcccaa accaaaataa tttcctttcc gtaaatcccc ggctcttccg ggggtccgcc
     3121 cgtggcggat tgacctttac agaatgcctg ggtcatggcc cgcgggcaag accctttcac
     3181 gcagaaagca tcggcagggt gggctctttt cccagaggct ccgctcgcct tccgcaaaag
     3241 gcagtgggcc ctgaggctac cgggcagatc gcccctgtaa ggaatcgacc cgaacacttg
     3301 gttcaggagt tgtgcccgcc cgttccatgg aatcaaaggc gccttatgat ttctgcgtga
     3361 aagggtcttg cccccgggcc ggcagaggga aggccacctt taggtggggc catttagaat
     3421 cagctttgag cggtcccctg ttttccaaac ctcctgccat gcccaaggta atgacttttc
     3481 aggctctaag gttaaggccg cgccttttgc ccacccccag ttcttgaaaa agagtccttc
     3541 gaaaaaaaga cctctggaaa ggagggggat ctcatggagt tcagggcgga gaaatttgga
     3601 tcatcatcct gaggcggtgg ttcttccatg ggatcgggcc tgtctagggg ggggattgaa
     3661 acggaaaaat gctttggctc tcttttctgt ttggaggaat ctcctctcag gcctctgcct
     3721 gcggaacatg gggaggagtt tcatggaagg ataagcgccc gccgagcctg atgatccaaa
     3781 ttccttcacc ctgaacccca gcaagcacga aaaactgggg ctgggcaagg attcgacaaa
     3841 gcagttgact ctcttcaaac cctatgctat cccaataaaa aaatcagggc gcagataatc
     3901 gagatagata tttgttgaat catgagccca gattattttt atagtcaccg acttaaaaag
     3961 ttgacgttgg gcg
//
LOCUS       DASPRR010000081         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1956428, whole genome shotgun sequence.
ACCESSION   DASPRR010000081 DASPRR010000000
VERSION     DASPRR010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956428"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VEL68_02810"
     CDS             <1..1906
                     /locus_tag="VEL68_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HXZ34931.1"
                     /translation="FHMLKNLVGEKIFFDSLKTFIEEQRFRPATWKDLQGAFERTRKK
                     DLEWFFRQWVEEKGLIEVDLGEVKVKPLDGKFEVEFEVRQKGRVYSFELPVSFYSDLG
                     LVRYRFPAEKESTRVKVLVDDYPRRIVLDEDYDLARTLSDTEFPPVIARLLSEERILL
                     IPPPSKEEVYQMAVDHFKKMGARIERAEQVKEEDLKASSAVVLDKKNPLAERLFGRIE
                     AAGGFSLILKENPWNPKKVIAILDAESSGELAAAFPKIRHYGKFSLVSFERGENVAKR
                     TESGSRGLGKDLVGETTAVEISTLKSLPQVIRQVADKRIIYVGETHDRYSHHLVQLEI
                     IRALHGMGKKLAIGMEMFQRPFQKALDDYLNGRIDERAFLKNSEYFKRWNFDYNLYRP
                     ILQLARAEKIPVVALNLQREITEKVGKTGLDSLSGEEKREIPSQMDFSDQAYRKRLEK
                     IFREHTSFEVRDFDFFLQAQILWDETMAESIDRFLMGNPDCHMVVLAGTGHLAHGSGI
                     PNRAARRNGFDYAIILNDAEWEKDIAHFIVNPAPVPFEGTPRLMVSLEEEKGQVVIQG
                     FSHGSVSEKAGMRKGDIILSFDGTPVQRVEDIRIELLFRKKEERVTVRVLRKEPLWGD
                     QAMEFEVPLH"
     gene            2361..>4053
                     /locus_tag="VEL68_02815"
     CDS             2361..>4053
                     /locus_tag="VEL68_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ34932.1"
                     /translation="MKKILLGNEAIARGLLESGCCFAASYPGTPASEILSSFVQMAKA
                     AESPAMGEWSINEKVAFETALAVSYSGGRAAASMKQVGLNVACDPLMSSAYTGVKGGF
                     LVISADDPGPHSSQTEQDSRMMAMTAKVPVLDPSSPQEAKEMIQLGFQTSEEFEIPVM
                     IRPTTRVCHSRQGVVLGEIPTPSIPTGFLKDPQRWAATPRYRFVLHKQLNQKIEAIAE
                     KYGEKSFFKILGGKNASQCILSSGVALAHTYDLLKELKLENQIALYQVKMPYPLNVRA
                     VLEKINSYKDILVIEETDPVMEMQLARRDRVKGRMDGSIPPQGELIPDVLDECIRKFA
                     GRTRETRPAPPGKPRRPTLCPGCPHRAAFYAIRLTFPNGIYPSDIGCYTLGLNLGAVD
                     TVLCMGATISQAAGFYHAYRMKGEFPPIVATIGDSTFYHAGLPPLVNAVHNGAKFILV
                     LLDNSTTAMTGNQPTPGMEVLVDGRKGKAVSMEELVKASGVKSLSVVDPYSLEEMTRA
                     LKEADKFCRSEEGGVAAIITRHPCLMDLGLERKKRTERVEISDDCIGCRVCIDDFE"
BASE COUNT          978 a         1061 c         1180 g          834 t
ORIGIN      
        1 cttccacatg ttgaaaaacc ttgtgggaga gaaaatcttt ttcgattccc tgaaaacatt
       61 catcgaagag caacggttcc gccccgcaac ctggaaggac cttcaggggg ctttcgagag
      121 gactcggaag aaagacctgg aatggttctt ccggcagtgg gtagaagaaa aaggactgat
      181 cgaggttgac ctgggggagg tcaaagttaa gcctctggat gggaagtttg aggtggagtt
      241 tgaggtccgg caaaaaggga gggtgtattc ctttgaactt ccggtttcct tttattcaga
      301 cctcggcctg gttcgatatc gattcccggc ggaaaaggaa agcacccggg taaaggtcct
      361 tgtggacgat tatccccgca ggatcgtact ggatgaggac tacgacctgg cccggacctt
      421 gtccgatacc gaattccccc cggttatcgc ccggttgctg agcgaagaaa gaatccttct
      481 catcccgccc ccttccaagg aagaggtcta tcaaatggcc gtcgatcact tcaagaaaat
      541 gggggcgaga atcgaaaggg cagagcaggt aaaagaggaa gacctgaaag cctcttccgc
      601 agtggtcctg gataaaaaga accctctggc ggagaggctg tttggccgga tcgaggctgc
      661 agggggcttc agcctgatcc taaaggagaa cccgtggaac cccaagaagg tcatcgccat
      721 cctcgacgca gagtcgtccg gggagctggc cgcggctttt cctaaaatcc gccactacgg
      781 gaagtttagt cttgtgtcct ttgaacgcgg ggagaatgtg gccaagagaa ccgaatccgg
      841 gagccgggga ttgggaaaag acctggtggg agagacgacc gcggtggaga tttccaccct
      901 gaaaagtttg ccccaggtca tccggcaggt ggccgacaag aggattattt acgtgggaga
      961 gacccacgac cggtactccc accacctggt ccagctggaa atcatccggg ccctccatgg
     1021 gatggggaaa aagctggcta tcggcatgga gatgttccag cggcccttcc agaaagccct
     1081 ggacgattac ctcaacggcc ggatcgatga aagagctttc ctgaaaaata gcgaatattt
     1141 caagcgatgg aatttcgatt ataacctcta ccgtcccatt ctccagttgg cccgggcaga
     1201 aaagatcccg gtggtggctt tgaacctcca gcgggaaata accgaaaaag tggggaagac
     1261 cggcttggat tccctttcgg gcgaagagaa gagggaaatt ccctcccaga tggatttttc
     1321 agaccaggcc taccggaaaa ggctcgaaaa gatctttcgg gagcatacct cttttgaggt
     1381 cagggatttt gacttcttcc tccaggccca aattctttgg gatgagacca tggccgagtc
     1441 catcgaccgc tttttaatgg gaaacccgga ttgccatatg gtcgtcctgg cgggaaccgg
     1501 ccatctggcc catgggtcgg gaattcccaa tcgggcggca aggagaaacg gcttcgacta
     1561 cgccattata ttgaatgatg cggaatggga aaaagatatc gcccatttta ttgtaaaccc
     1621 cgcccccgtc cccttcgaag gaaccccccg gctcatggtg agtctggaag aggagaaggg
     1681 gcaggtcgtt atccagggct tttcgcatgg gagcgtttcc gaaaaggcgg ggatgcggaa
     1741 aggggatatc atcctctctt tcgatggaac gcccgtgcag agggttgaag atatccgcat
     1801 cgaactcctc ttcaggaaaa aggaggaacg ggtgaccgtc cgggtgctca ggaaagagcc
     1861 cctttgggga gatcaggcca tggagtttga agttcccctc cactgaccag gcctttaaaa
     1921 aagccttgac atgcattgct gatgcgtagt aaaaaataaa aatgttgagg aaagttttta
     1981 tgggcagccc aattctgaat ctctggtggt ggtggtgcaa aaagagagga ggagcctgcc
     2041 cttaaaccgg ccaaattgac ctaatcgatt acccgaaaag ccgtgcgcag attccaacgc
     2101 tcacggcttt ttttttagcc acacagagta cagagaacac agcgcggcag agccgcgctg
     2161 tactgctaac ctgcaaactc ttcaatttta gtgaagcaaa tttccttttg gcaaatccct
     2221 tctgaaatcc cccccaaccc ccctttgtca aaggggggca agggggggat ttctgggggg
     2281 agcgcggtcc ggggcaagag atattttttg gttacggcta cgctgcactg tgttctctgt
     2341 gacaaaaaat aggagaaata atgaagaaaa ttcttttggg gaatgaggcc atcgcgaggg
     2401 gccttttgga gagcgggtgt tgtttcgcgg cctcctatcc cggaacgccg gcctcggaga
     2461 tcctttcctc cttcgtccag atggccaagg ccgccgagag cccggccatg ggggaatggt
     2521 ccatcaacga gaaagtagcc tttgagactg ccttggcggt tagctattcg ggagggcggg
     2581 cggcggccag catgaagcag gtcgggttga acgtggcctg cgatcctttg atgagctcag
     2641 cctacacggg tgtcaaaggc ggattccttg tcatctccgc cgatgatccc ggaccgcaca
     2701 gctcccagac cgagcaggac agtaggatga tggccatgac ggccaaggtc ccggtcctgg
     2761 acccttccag tccccaggaa gccaaggaga tgatccaact cggcttccag acctcggagg
     2821 aattcgaaat cccggtcatg atccggccca ccacacgggt ctgccactca cggcagggag
     2881 tcgtgctggg cgaaatcccg accccctcca ttcccacggg tttcctgaaa gacccccaga
     2941 gatgggcggc cactccccgc tatcgttttg tgctccacaa gcagttgaac cagaagatcg
     3001 aggcaatcgc ggaaaaatat ggcgagaaat ctttctttaa gatattgggc ggtaagaacg
     3061 cttcccagtg cattctttct tccggggttg ccctggccca cacctatgac cttctgaaag
     3121 aactcaagct tgaaaatcaa atcgcgcttt atcaggtgaa gatgccttat cccctgaacg
     3181 tcagggccgt actcgagaaa atcaattctt acaaagatat cctggtgatc gaagagaccg
     3241 atccggtcat ggaaatgcag ttggccaggc gggaccgggt caaaggaagg atggacggat
     3301 cgataccccc ccagggcgag ctgattccgg atgtcctgga cgagtgtatt cggaagtttg
     3361 cgggaagaac tcgggaaacg cgaccggccc cgcccggcaa accccggaga cccacgctct
     3421 gccctggatg cccgcaccgg gccgcgttct atgccatccg cctgaccttc ccgaacggga
     3481 tctatcccag cgacatcggc tgctatacac tcggtttgaa cctgggggct gtggacacgg
     3541 tcctgtgcat gggagcgacc atcagtcagg ccgcggggtt ctaccatgcc taccggatga
     3601 agggggaatt cccgcccatc gtagctacca tcggggattc cactttctac catgccggcc
     3661 tgccgccgct ggtcaacgcg gttcacaatg gggcgaaatt cattctggta ctcctcgaca
     3721 attccaccac tgccatgacc gggaaccagc cgacccccgg gatggaggtc ctggtggacg
     3781 ggcgcaaagg aaaagccgtt tccatggagg aactggtaaa agccagcggg gtgaaatccc
     3841 tgtcggtggt tgatccgtac tcgctcgaag aaatgacccg ggccctgaaa gaagcggaca
     3901 agttttgccg gtccgaagag ggaggcgtgg cggccatcat cacccggcac ccctgcctga
     3961 tggacttagg actggaaagg aaaaagagaa cggagcgggt ggaaatatcg gacgactgca
     4021 tcggctgccg agtctgcatc gatgattttg aat
//
LOCUS       DASPRR010000082        21825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_985859, whole genome shotgun sequence.
ACCESSION   DASPRR010000082 DASPRR010000000
VERSION     DASPRR010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 21825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21825)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21825
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985859"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VEL68_02820"
     CDS             complement(<1..280)
                     /locus_tag="VEL68_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34933.1"
                     /translation="MADTSRIWSGFFCVAMVLAAGIFLWGISAQSYWALAIPVMIGCL
                     GILAMGFWIGWTILTIKTAPPAAEPPSPPPAEAKKPSPTSSSEPEKGIP"
     gene            complement(282..1103)
                     /locus_tag="VEL68_02825"
     CDS             complement(282..1103)
                     /locus_tag="VEL68_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ34934.1"
                     /translation="MNHKDGGERPQVDLDWDDEGILPPVLSPDTEFVFQRMTEETLKA
                     AEGEFDYRALDVGCGRALDAAALARKGGILFGCEPSRIMLRKAREGLAKEEERVCLVS
                     SLAETLPFAGHSFHRVVCKGAIDHFLNPDRAVAEMSRVASAGGKVVISVANYESLSCF
                     LSRGLNRFVQKVWRREIPPPHIWEVPPDHTHKFDYPTILGLAKKYLRMESIRGVSLLW
                     GFPRWSSFLQRIPQPMALILLRGLDKIANWVPDWSDVLILVGRPHDPLPEKERSS"
     gene            complement(1093..2142)
                     /locus_tag="VEL68_02830"
     CDS             complement(1093..2142)
                     /locus_tag="VEL68_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXZ34935.1"
                     /translation="MNRRSILILYSLPHPFLPDGSVDIFGPKSVLSRLQAVKESLLSL
                     GYTIKVLKMEDELSPLVSKILRSGAELIFNLCEEFHGQTRLEMNVAALLELLPIPFTG
                     SSALVLGLSQDKGKTKSILGHYGIPTPRYIIWPPGERVSTSRLKKPLFVKPLREDASL
                     GIGNDAIARNLPSLLSKVAKVHRLHHQPALVEEYIAGRELNVSILGNEAAQVLPISEI
                     DFSTLPKGLPKICGYEAKWVEDSPEFHHTIPLCPAPLPMKLEKRVREVALQAYRAMEC
                     RDYGRVDIRLSPDGTPYVLEINANPDISPDAGMPRSAKAAGLAYPELIGRIVELAWAR
                     ARSSKGFPGESTHES"
     gene            complement(2139..3146)
                     /locus_tag="VEL68_02835"
     CDS             complement(2139..3146)
                     /locus_tag="VEL68_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXZ34936.1"
                     /translation="MRVGFCYNLKKQADESTPADLYAEWDDEETIGAVHSALARRHEV
                     IPIEGDEGAFEKFRETRPDIVFNMAEGIFGASRESQIPAMLEMLRLPYTGSDPLTLAV
                     CLDKAMAKVALSYFGVPNAPFVVVPELKKKVEFPPFPLIVKPLREGSSKGIQNSSLVR
                     NSRRLKEEVRRVLNEYRQPALVEKFLPGREFTVALLGNGPQVEVLPIVEVQFEELPSH
                     INPIYSYEAKWVWDTVENPLEIFACPARIPISLRRRIESVCRAAYVALGVRDWCRIDV
                     RLDALGNPNILELNPLPGILPNPDANSCFPKAARTAGLTYEELINRVLDIACRRVGLK
                     A"
     gene            complement(3167..3712)
                     /locus_tag="VEL68_02840"
     CDS             complement(3167..3712)
                     /locus_tag="VEL68_02840"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HXZ34937.1"
                     /translation="MEPLVPRKLFFTKGVGTHKEELRSFEFALRDAGIEKCNLVHVSS
                     ILPPRCKIISRTQGLKELTPGGITFCVLARCSTNEPRRLIAASIGCAIPSDRNQYGYL
                     SEHHAYGQTEKEAGDYAEDLAAAMLASTLGIEFHEDKSWDERKEIYRISDKIVRSSNV
                     TQSNIVKTGWSTVVAAAVFLF"
     gene            complement(3731..4633)
                     /gene="speB"
                     /locus_tag="VEL68_02845"
     CDS             complement(3731..4633)
                     /gene="speB"
                     /locus_tag="VEL68_02845"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HXZ34938.1"
                     /translation="MTGKFSTVPFNFGGLPHDQCRWEKSRVVFLPVPYDLTSTYLPGS
                     RRGPLAILEASTHLELFDEVLEQETYRVGFHTLDSLEAVATGPQGMIALVEKYVQKVV
                     RAGKFPVLIGGEHSLTLGAARALKKKHPRISFLQLDAHADLRESYQGTSFSHACAGRR
                     LTEIGPLVQVGIRSLTQDEHLYLRKGKVKTFFWHHLAQTPDWQEEVCGSLLPEVYVTI
                     DLDVLDPAIMPAVGTPEPGGLDWKSLTNLLRLVAGMKKVVGFDVVELTPIPGLIAPDF
                     LAAKLIYRFLGEVFVPSFRRGKRF"
     gene            4873..6174
                     /gene="purB"
                     /locus_tag="VEL68_02850"
     CDS             4873..6174
                     /gene="purB"
                     /locus_tag="VEL68_02850"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXZ34939.1"
                     /translation="MIPRYTRKRMADVWEAENRFRKWLQIEVSACEAMSKLGLVPAAA
                     LKEVRKKAGFDVARIEEVEKIVKHDVIAFLTVVSERVGPAARYLHLGMTSSDVLDTSF
                     AMQLVEACGILEEDLRALLAVLKKRALQHRHTPIIGRTHGMHAEPTTFGLKLALWYAE
                     MERNLERLERAKERVAYGKVSGAVGTFAHLPPSVEAYVCKRCGLKPAPISNQIIQRDR
                     HAEFFTTLALIGSSIEKIAVEIRHLQRTEVAEAEEPFTAGQKGSSAMPHKRNPIASEN
                     LAGLSRVLRGNALAAMENVVLWHERDISHSSVERVIGPDSTTLLDYMLNRLTHLIANL
                     VVYPQNMKANLEKSRGLVFSEGILLRLVQKGLTREESYALVQKAAFKALKAKRNFETI
                     LIRDRAILRYLKPAEIKESLNLAHTLRHADEIFRRVFVKRK"
     gene            complement(6246..7460)
                     /locus_tag="VEL68_02855"
     CDS             complement(6246..7460)
                     /locus_tag="VEL68_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ34940.1"
                     /translation="MKKNVWFTVTALILAIVWIPGSALGAEKTLKLGASVCITGRLAK
                     EGGYVKDGYTLWMEMVNQKGGIKVGPDRYRVEIVFYDDKSDPQTGAKLTEKLITEDKV
                     KFILGPYGSGITLATSAIGEKYRVLTVACQANAVNIYERGFKYIISILPPATYYLRPV
                     VDLSQKINPPVKTAAVIWADDAFPEYCGKGAAAYAKERGLQVVYEGKYPKDVKDLSSI
                     LLEAKNRKPDFLLGGTYFNDGLLITKQMKEQNFDVKMIGLSVGPQLPAFVENLGRDAE
                     YVMGCEWWTPYSPWKDDFFGTAKEYAQKMKARFGYEPSYHPTSASVAGYVLQLAIEKA
                     QSMDVDKVTETLRNMKVTTVWGPMAWNKAGQNTEIHSAAVQVLKGKMEVVYPFEGKTA
                     NLVYPFVPWGKR"
     gene            complement(7568..8278)
                     /locus_tag="VEL68_02860"
     CDS             complement(7568..8278)
                     /locus_tag="VEL68_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34941.1"
                     /translation="MMLEIQSLNLGYGDIQVLWDIHLRIAEKEVVALAGSNGAGKSTL
                     MKGIIGILKPISGNIQFFGKEVSPLPPSKRVPLGISLVPEGREIFYWMSVEENILMGA
                     YTRADVDGIQKSLEWVYNLFPRLKERRTQQAGTLSGGEQQMCALARGLLADPKLLLVD
                     ELSLGLAPVMVDRLVAAIREVHQTGQISIFLVEQDVQIAFELASRGYIIETGKIVAED
                     TSPRLLADNRIREAYLGI"
     gene            complement(8275..9033)
                     /locus_tag="VEL68_02865"
     CDS             complement(8275..9033)
                     /locus_tag="VEL68_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ34942.1"
                     /translation="MALLEGKDVTRAFGALKAVDAVDFQVREGEILGLIGPNGAGKTT
                     LVNLVTGTYPLTEGEISFKGTPITGLKPAKVSRLGIARTFQIVQPFPGMSLRENVVVG
                     ALFGKNGAGRSTKEAFREADRWIDFVHLQDYRNAGVDQINISFRKRMDLAKALAMNPE
                     VLMLDEVMAGLNTKDIEEMIALVRRINEELKISLLVIEHVMKAVMTLCHRVTVLHHGG
                     KIADGSPQEVVKDPRVIEAYLGERYAKARKGSET"
     gene            complement(9041..10009)
                     /locus_tag="VEL68_02870"
     CDS             complement(9041..10009)
                     /locus_tag="VEL68_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34943.1"
                     /translation="MGGQKKILLIFGLIAVLGLIVLPFFLEGYWIRVITSIFMYAIIT
                     AALNIIAGFAGYAAFGNIVFFGIGAYTTAMLMLRVGLYWWVGAIVGGVLASIYAILLG
                     IPILRLRGHYFAIATLGINRATEQIALDWGSFTGGGKGITLPLPQMEINTFYVMIYFI
                     MFGLLAFTVLVNYGIARSRLGYALRAIRENEGAAEASGIFAPRCKIVAWAISAFITGI
                     TGGVFAYWFSFIEPATVFDIMIAVKAFVMMMLGGAGTVLGPVLGAFLIELISETVWGK
                     FLTVHMLILGIIMVAIVILMPRGIMEFFRKKFSLAAIWNNIKNQAV"
     gene            complement(10093..10953)
                     /locus_tag="VEL68_02875"
     CDS             complement(10093..10953)
                     /locus_tag="VEL68_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ34944.1"
                     /translation="MSLQILLNGIFLGAIYVNIAVGFSLAWGVMDVINVAHGTMIVVG
                     AFVTFACFKAFGLDPFLSLPISMAILFIIGYGAQKYLLNLVVKVGIFLSLILTFGLDL
                     VIINALLMIFTGDYRSLTPSYSGSSFQIGSLVIPYVRLAILVICILLTALLQLFLSKT
                     RTGNAIRATSQNRQAAQIVGVDVAKVYSITLGISAALAGACGSLISTIQSFTPFEGGF
                     FIMKAFIICVLGGLGNVWGAIFGGLLLGVVETTGASIFGTGFQEAISAIIMILVLIFL
                     PKGIVGSKYK"
     gene            11250..12074
                     /locus_tag="VEL68_02880"
     CDS             11250..12074
                     /locus_tag="VEL68_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXZ34945.1"
                     /translation="MNQSLAHGLQILLLFDSTRPALTVPEIVEHLRFSQSKAYRLIRA
                     LVYYGLLETQNGTGQYSLGMSALRLGFLAQRKLNLSALALPFMKELSRLTKETVLLTV
                     VQGTRAICLERVESEEPIRYSLFTPGASLPLHAGASSKVLMAFLPEREWGPIIAREGL
                     PRFTPQTITDRKILTSQLREIRCRGYAFSDQEVDRDVRAVAAPILNPRGELIAGLTVA
                     GPAYRIHKRRVQSLGRLVRQSAQKIAGRLQGEPLLPAPQGNCKFIRKKSILIQKGV"
     gene            12076..13008
                     /gene="argF"
                     /locus_tag="VEL68_02885"
     CDS             12076..13008
                     /gene="argF"
                     /locus_tag="VEL68_02885"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXZ34946.1"
                     /translation="MAVNLKGRSLASLYDLTREELEQILKTSELLKLENSRGQEHPLL
                     KGKTLAMIFEKASTRTRVSFEVGMWQLGGYALYLSAGDLQLGRGETIADTARVLSGYV
                     NGIMARVFAHQTILDLVRFSRVPVINGLSDYSHPCQGLADLFTIYEKKGRVSGLNVAY
                     VGDGNNVSHSLLYGCSKMGAHIVIASPKGYEPKEEVISRAVEEGRKNGSTVKVTQDPH
                     EAVRGADVVYTDVWTSMGQEKEKEQRLRLFQPFQINPKLLRGAGEDYVFMHCLPAHRG
                     EEVTDEVADSKNSVIFQQAENRMHTQKALLALTL"
     gene            13137..14495
                     /locus_tag="VEL68_02890"
     CDS             13137..14495
                     /locus_tag="VEL68_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ34947.1"
                     /translation="MAKKFDVLIQGGLVVSGSRIRRADVGVQGEKILTVAEGLSAADA
                     VQTIDAAGKFVFPGILDVHTHPVYEDNIQGLSRTAAHGGTTTLIFYAYARPGMKLIDT
                     IKAYREDGLKNSFLDFALHGAIFDAASQVPEIPKAFELGVKSFKMFMTYAKLKWMTDD
                     YHLTWAMDLISENGGLAMVHAESGLVTDYIEDKSLRRKEDQKEVFVKTRPDYLEAEAV
                     FRAISIAAVMQCPIYIAHLSSARGVFPIQQARGEGQWVYAETCPQYLSLTDEILQKTG
                     PLAKIGPPLRYETDRMALWDAVEKGTIDTIASDHAPKAKRITDSFFEAPFGAPSAETL
                     LTVTYDEGVNTGRIGACKLVQLLCENPAKIFGLFPRKGILQEGADADVVVFDPVLAHT
                     IEQRTQHSGAPYTMYEGRSCLGKPVFIMQRGKALVDNGEMKMKPGEARFCPTKIEKVK
                     LW"
     gene            14648..15571
                     /gene="pyrB"
                     /locus_tag="VEL68_02895"
     CDS             14648..15571
                     /gene="pyrB"
                     /locus_tag="VEL68_02895"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HXZ34948.1"
                     /translation="MAGLKGQDILHGNQFSRKDIEGIMKVASAFEKEGKGALNVLKGK
                     ILATLFFEPSTRTRLSFEAAMQRLGGGVISLGSLESSSVAKGESLTDTAQTVAQYADV
                     IVLRHPRQGSAKEAADAVSVPVINAGDGAGQHPTQALLDMYTIRKELGSLKNLKVCLA
                     GDLKYGRTVHALAEVLSLFGARLYFVSPSMLRMPEEITAGLRSRGIEVTETEDLLQAA
                     SLSDLIYMTRIQKERFSDQGEYERVKGSYVINNRFLSQLKKEITLMHPLPRVDEIHPE
                     VDGYAGSAYFRQMRNGVFIRMALLAMIFGKR"
     gene            15576..16526
                     /gene="arcC"
                     /locus_tag="VEL68_02900"
     CDS             15576..16526
                     /gene="arcC"
                     /locus_tag="VEL68_02900"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HXZ34949.1"
                     /translation="MKKIVIALGGNAIKQWDQKGSAEEQLANVRQSCTHIVRIIRGGN
                     RVVITHGNGPQVGSLLIQQERGSPAIPPQPLNICGAMSQGQIGYMIQQALLNELKDAR
                     LDRSVVTLITQVVVDGEDPGFRQPTKPVGPFYSRAQKEKYEKQTGAAFQRTPMKKELY
                     RRVVASPDPLRILEGNMVKDLFERGVIVIASGGGGVPVILDENGHVSGVEAVIDKDLA
                     GEKLAEAVGADIFMVLTDVDRVYLNYGKRSQRGLSTLRLEKAQQHLKNHAFPSGSMEP
                     KVAACVRFIEYGGERAIITSLDQASAALEGRAGTHLLPDE"
     gene            16537..17478
                     /gene="pyrB"
                     /locus_tag="VEL68_02905"
     CDS             16537..17478
                     /gene="pyrB"
                     /locus_tag="VEL68_02905"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HXZ34950.1"
                     /translation="MRLNHILEAQQFNKKMLKEVFTLADQMAALAQGGGSDLLKNRIL
                     ASLFFVPSNRTRFSFESAMIRLGGQILGSDRAEAFSSELDGGSLEDTITTLNYFADVI
                     VLRHHLSGSAKRAARVSNVPIINAGDGTAQHPTQALLDLYTIERELGGVDGVVIAFVG
                     DLANSRNVRSLCYFLAKYTGIKVHFVAPQPLGMRDDLCQYLRSQGVTFVEAYGPEPPL
                     LDLARKVDVFYVTQIPVGSFGDRLDDYEASKRQFVIDRRVLKAMKKNSIIMHPLPRGP
                     EIPPEMDKDLRAVYFQQTRYGLCIRMALLCAVFQRFP"
     gene            17753..18610
                     /locus_tag="VEL68_02910"
     CDS             17753..18610
                     /locus_tag="VEL68_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXZ34951.1"
                     /translation="MKKIVFGFCWGVMGLAFLLVFSPPGGAQDKPLVIGVDGTFAPHA
                     MPKIGGGVEGFNVDLALEIGRRMGRKVEIVSQQFSGLIPGLNAGKFDFLAAPTTVLKP
                     RDKNLLFTEGYLETDYQFVIPIKAAEIKQFEDLRGKVVAVNKGSNYEMWCQENGPKYG
                     FKTESYGTNTDAVQAVLAGRSDTNLAGHTVAAYAVKKNPGQLKLSYKIKSGAVWSIPF
                     RKDDVQMRKVVEETLECMKMDGTVAKLAEKWFGVKPDKDSPAVTVYPGYGVEGFEGYD
                     PTPHTPACK"
     gene            18682..19434
                     /locus_tag="VEL68_02915"
     CDS             18682..19434
                     /locus_tag="VEL68_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXZ34952.1"
                     /translation="MNPSAPEPILKVSGLCKSFGTLQVLKGIDLQVLPRELIFIIGPS
                     GSGKSTLLRCCNRLEEPTSGSIRIEGVDILAPGVDINLVRRRIGMVFQSFNLYPHLNA
                     LGNITLALRKVLKKSKTEADDIAFKALGRVGLSEKARTFPNELSGGQQQRVAIARALA
                     LEPAIMLFDEPTSALDPELVGSVLNVMRQMRESGMTMVVVSHEMRFARGAADRVIFMD
                     EGMILEQGAPEKIFNEPDHPRIKEFLSSLEAK"
     gene            19587..20261
                     /locus_tag="VEL68_02920"
     CDS             19587..20261
                     /locus_tag="VEL68_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXZ34953.1"
                     /translation="MLKSFLFTYFNLPVMAEYLPQILEGFILTVELAAAIVVTGILAG
                     FMLAVLRSFRVRFLNFFIVIFVDVLRALPPLVIIILFYFALPSVGVRMSGFAATWLSL
                     SLVLTAFAEEIFWAGILAVPKGQWEAARSTGMSFLQTLGGVVLPQAVRITIPPLTNRT
                     IAITKGTALGAVVAVGEILNQAQTGLSHSYNPSPLTLGALAYLVLFIPVVTLGRWVET
                     RFAWKR"
     gene            20265..20936
                     /locus_tag="VEL68_02925"
     CDS             20265..20936
                     /locus_tag="VEL68_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXZ34954.1"
                     /translation="MEQIIQGFFNIEIVIAAFPFLLRGLAMTVLLCLVVIPLGLFGGL
                     SLALVSTVRSVLAKGLVVVYVDFFRAFPPLVLLIFIYFGVPFLGIDLHAFTAVTIGFF
                     LNTSSYYGEIFRAGIESIPRGQREAARSTGLTSVQTMVFVVIPQATRNVIPDLVSNTL
                     EVVKLTSIASVVALPELLYAARQAQSITYNATPIIFAGMVYLICLWPLVRLLRRFEHK
                     KVALH"
     gene            complement(21026..>21825)
                     /locus_tag="VEL68_02930"
     CDS             complement(21026..>21825)
                     /locus_tag="VEL68_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HXZ34955.1"
                     /translation="GVGYDNVDLAAAGEKGIYVCNVPDYGTDEVADHAIALMLACARG
                     LTFTERRLRKTLSPWNYLAATPLRRLAGSTLGIIGLGRIGTATALRAKALRMKVVAND
                     PYLADGYDKALGVALLSLDELLAVSDVVSVHTPLNPETTGIVGAPQLTRMQPHAILVN
                     TARGKCVDAGAVAEALKAGKIGGAGLDVLPEEPPGAEDPIVQLWQQEDPPVNLILTPH
                     NAFYTEQSREEMRTKAALEVRRILLGEKPRNPVNLEILKAASPGRRA"
BASE COUNT         4746 a         6150 c         6330 g         4599 t
ORIGIN      
        1 agggaatccc tttttcgggt tctgaggagc tggtgggcga aggctttttc gcctccgccg
       61 gtggtggaga aggaggttcg gccgccggag gagcggtttt gatggtgagg atagtccagc
      121 cgatccagaa tcccatggcc agaattccca ggcacccgat catgaccggg atcgccaggg
      181 cccaatagct ctgggccgaa atcccccaga ggaagatccc tgcggcaagg accatggcga
      241 cgcagaaaaa accactccaa atccgagagg tatccgccat ttcaggaact cctttctttt
      301 tccgggagag ggtcgtgcgg cctgcctaca aggatcagga cgtcgctcca atccggtacc
      361 cagttggcga ttttatctag cccgcgaaga agaatcaagg ccatcggttg aggaatccgt
      421 tggagaaagc tcgaccaccg gggaaatccc cacaggaggg aaactcctcg gatggattcc
      481 atccgcagat attttttggc caaacccaag atcgtgggat agtcgaattt atgagtgtgg
      541 tccgggggaa cttcccagat atgaggcggg gggatctccc ggcgccagac cttctgaacg
      601 aaccgattga gcccgcgcga gagaaaacaa ctgaggctct cataattggc caccgagata
      661 accacttttc ccccggccga agccacccgc gacatttccg caaccgctcg gtcggggttt
      721 agaaaatgat cgatggctcc cttgcatacc acccgatgga aagaatggcc ggcaaaggga
      781 agggtttccg ccaggctgct gaccaggcag actctctcct cttctttggc cagcccttcc
      841 ctcgctttcc gcagcatgat cctggagggc tcgcacccaa agaggattcc ccctttccgg
      901 gcaagggcag cggcgtcgag ggcccgcccg caacccacgt ccagggcccg gtaatcaaat
      961 tccccctcgg cggccttcag ggtctcctcc gtcatccgtt gaaatacgaa ttccgtgtcc
     1021 ggagaaagga cagggggcag aatcccttca tcgtcccagt ccaaatcgac ctggggtcgc
     1081 tcccccccat ccttatgatt catgggtgga ttcccccggg aaacccttag aggagcgggc
     1141 tctggcccag gccaattcca cgatccggcc gatcaattcg ggataggcca ggccagcggc
     1201 tttcgccgac cggggcatgc ctgcatcggg ggaaatgtcc ggattggcgt tgatctccaa
     1261 cacgtagggg gttccatccg gactgagacg gatgtccacc cggccatagt cccggcactc
     1321 catggcccgg taagcctgaa gggcgacctc tcgaacacgc ttttccaatt tcatcggaag
     1381 gggggctgga caaagaggga tggtatgatg aaactccggg ctgtcctcca cccacttggc
     1441 ttcgtagccg cagattttcg gaaggccctt gggaagggtg gaaaaatcga tctccgagat
     1501 gggaaggacc tgggcagcct catttcccag gatggacacg ttcaattccc ggcccgcgat
     1561 atattcctcc accagggccg gctgatgatg gagccgatgg accttggcca cttttgaaag
     1621 gagggaggga agattccggg caatggcgtc gttgccgatc cccaggcttg catcttcccg
     1681 caggggcttg acaaagaggg gctttttcag gcgggaagtg gaaactcttt ccccgggagg
     1741 ccaaatgatg taacgggggg tcgggattcc gtagtggcct aagatggatt tggtcttccc
     1801 tttatcctgg gagagaccca ggacgagagc agaggacccc gtgaatggaa taggtaagag
     1861 ctccagcagg gcagccacgt tcatctccag ccgggtctga ccgtggaatt cttcgcagag
     1921 gttaaaaatg agttcagccc cagaccgcag tatcttgctc accaaggggg agagttcatc
     1981 ttccattttc aagaccttga tggtgtagcc cagggagaga agtgattcct tcaccgcctg
     2041 gagacgggaa aggacagatt tgggtccgaa gatatcgacc gatccatcgg ggagaaaagg
     2101 atggggaagg ctgtagagaa taaggatcga acgtcgattc atgctttcaa ccccaccctc
     2161 cggcaggcga tgtccaggac gcggttaatg agctcctcgt aagtcagacc ggccgtgcgg
     2221 gcggctttag ggaaacagga attggcatcc gggttgggaa gaatcccggg aaggggattg
     2281 agttccagga tgttcggatt tcccaaggca tccaggcgga cgtcgatgcg gcaccaatcc
     2341 cgcaccccca gggcgacgta cgccgcccgg cataccgact caatcctcct gcgcagggat
     2401 attggaatcc gggccggaca ggcgaaaatt tccaagggat tttccaccgt gtcccagacc
     2461 catttggcct cataggagta aatcgggttg atgtgcgacg gaagttcctc aaactggacc
     2521 tccacaatgg gcaaaacttc gacctggggc ccgtttccca gcagggcaac ggtaaactcc
     2581 cttccgggaa ggaacttttc caccagggcg ggctgccggt actcattcag aactcggcgg
     2641 acctcttctt tcagccgccg ggagttgcgg accaaggagc tgttctggat tcccttgctg
     2701 gacccctccc gtaggggctt tacgatgagg gggaaaggag gaaattcaac cttcttcttt
     2761 aactccggga cgaccacgaa gggagcgttg gggaccccga agtaactgag ggctactttg
     2821 gccatggctt tgtccaggca gacggccagg gtcagagggt ccgagccggt gtagggtagg
     2881 cggagcatct ccagcatagc cgggatctgg gactcccggc ttgccccaaa aatcccttcg
     2941 gccatgttga agacgatatc cgggcgggtt tcccggaatt tttcgaaagc cccttcatcg
     3001 ccctcgatgg gaatgacttc atgcctgcgg gccagagccg aatggaccgc cccgatggtc
     3061 tcctcatcgt cccactcggc gtacaagtca gccggggtgg attcatcggc ctgttttttc
     3121 aggttgtagc aaaagcccac tctcatcgcc tttcctccag cctggctcag aagagaaaga
     3181 ccgccgcagc caccaccgtg gaccagccgg tcttcacgat gttggactgg gtgacgttgc
     3241 tggaccgaac gatcttatcg ctgatgcggt agatctcttt tctctcgtcc cagctcttgt
     3301 cctcatggaa ttcgatgccc agggtggagg ccagcatggc cgcagccagg tcctcggcgt
     3361 aatcccccgc ttctttttcg gtctgcccat aggcatggtg ctcgctcagg tacccgtact
     3421 ggttgcggtc cgaggggatg gcgcagccaa tggaggcggc aatcaggcgg cgcggctcat
     3481 tggtgctgca cctggccaga acgcagaaag tgatccctcc cggggtcagc tccttgagcc
     3541 cttgcgtgcg ggagatgatc ttgcagcgag ggggaagaat gctggagaca tggaccaagt
     3601 tgcatttctc aatgcccgca tcccgcaggg caaactcgaa ggagcggagt tcttccttgt
     3661 gcgtgccgac ccccttggtg aagaatagct ttcgagggac taggggttcc aattcctatc
     3721 tcctttgttg ctaaaatctt tttccccggc ggaatgaagg aacaaagacc tcgcccagaa
     3781 aacggtagat cagcttggcc gccaggaagt cgggagcgat cagcccggga atgggggtaa
     3841 gctccaccac gtcgaagcca accaccttct tcatgccagc caccagtcgc agaaggttgg
     3901 tgagggactt ccagtccaac cctcccggct cgggggttcc caccgcgggc atgatggccg
     3961 ggtcgaggac atccaggtcg atggtcacat acacctccgg gaggagagag ccgcagactt
     4021 cctcctgcca atcgggggtc tgcgccagat gatgccagaa aaaggttttc acctttcctt
     4081 ttcggaggta gagatgttca tcctgggtga ggctccggat tcccacctga accagcggcc
     4141 cgatctcggt caggcgccgg cccgcgcagg catggctgaa agaagttccc tggtaagact
     4201 cccgcaggtc cgcatgggcg tcgagctgaa gaaaggaaat ccggggatgt ttctttttca
     4261 gggcccgggc cgctcccagg gtgagggaat gctccccacc gatgagcaca ggaaatttgc
     4321 ccgcccggac caccttttgg acatactttt ccaccagcgc gatcatcccc tggggaccgg
     4381 tggccaccgc ctccagggag tccagggtat gaaaccccac ccggtaggtt tcctgctcca
     4441 gcacctcgtc gaatagttcc agatgggtgg atgcctccag gatggccagg gggccccgac
     4501 gggagccggg caggtaggtg ctggtaaggt cataaggaac cgggaggaag actacccggg
     4561 atttctccca cctgcattgg tcgtgaggaa ggcccccgaa attaaatgga acggtggaaa
     4621 acttcccggt catctcctcc tcccggagag aaattcattt ttatcgattc taaaaattac
     4681 ttaaaaaaaa ttctttcgtc aagaaatata attatgaaag cgatcttttt tattcctgtt
     4741 catggtttcc ctgctttttc tttggccgag tctcacggtc ctgaaggtat gcgggggcag
     4801 gtcacctttc gattccttga cttccgccgg caaacctgtt aaggttcgct caaaaaaatc
     4861 agaggagcag tcatgattcc ccggtatacc cgcaagagaa tggccgatgt ttgggaagcg
     4921 gagaatcggt ttcgaaaatg gcttcagatc gaagtctcgg cctgcgaagc catgagtaaa
     4981 ttggggctgg tcccggcggc agcgctaaaa gaggtccgca agaaagccgg attcgatgtg
     5041 gcccggatcg aagaggtgga aaaaatcgta aaacacgacg tcattgcctt cctgaccgtg
     5101 gttagcgaaa gggtaggacc ggcggcccgc tacctccatc tgggcatgac ctcctcggat
     5161 gttttggaca cctcctttgc catgcagctg gtggaagcct gcggaattct ggaagaggac
     5221 ctcagggctc ttctggccgt gctgaagaaa agggctttgc agcaccgaca tactccgatc
     5281 atcggacgaa cccatgggat gcacgccgag cccacgacct tcggcctcaa gctggcccta
     5341 tggtacgcgg agatggaaag gaacctggaa cggctggaga gggccaaaga gcgagtggct
     5401 tatggaaagg tctccggagc cgtgggaacc ttcgcccact tgcccccgtc agtggaagcc
     5461 tacgtgtgca aaaggtgcgg cctgaagccc gcgcccatca gcaaccagat catccagcgg
     5521 gaccgccacg ccgaattttt cacaaccctg gccctgatcg gaagctccat cgagaagatc
     5581 gccgtggaga tccgccacct gcagcggacg gaagttgccg aggctgagga accctttacg
     5641 gccggccaga aaggatcttc cgccatgccg cacaagagaa acccgatcgc ctcggaaaac
     5701 ctggccggtc tctcgcgggt gcttcgcggg aatgctttgg cggccatgga aaacgtggtc
     5761 ctctggcacg agcgggacat cagccactcc tccgtggagc gggtgatcgg cccggacagc
     5821 acgactctgc tagattacat gctgaatcgt ctaacccacc tgattgccaa tttggtcgtg
     5881 tatccccaaa acatgaaggc gaatctggaa aagagcaggg gcttggtttt ttcggaagga
     5941 atcctcctcc gactggttca aaagggtctg acccgagaag agagctatgc tttggtgcag
     6001 aaggccgcct tcaaggcgct aaaggcaaaa cggaattttg aaaccatcct gattcgtgat
     6061 cgcgccattc ttcggtatct aaaacccgca gagatcaagg agtccttgaa ccttgcccat
     6121 acgcttcgcc atgccgatga aattttccgg agagtttttg tgaaaagaaa atagataagg
     6181 tttttcgaaa gaataagaaa ggggaggcct ggcagcctcc ctttttttat tccgcgctcc
     6241 cttggtcatc tttttcccca aggaacgaag ggatatacga ggttggcggt ttttccctcg
     6301 aaggggtaga cgacttccat tttccccttg aggacctgga ccgcagcact gtggatttcg
     6361 gtgttctgcc cggctttgtt ccacgccatg ggtccccaaa cggtggtgac cttcatgttg
     6421 cgcagggtct cggtgacctt gtccacatcc atggattggg ccttttcaat agccaactgc
     6481 aggacgtagc cggccacgct cgccgatgtg ggatggtagc tgggctcgta gccgaagcgc
     6541 gccttcatct tctgagcgta ttccttggcc gtaccgaaga agtcgtcctt ccagggggag
     6601 taaggggtcc accactcgca gcccatcacg tactcggcgt ccctgccgag gttttccacg
     6661 aaagccggga gctggggtcc tacggaaagg ccgatcattt tcacatcgaa attctgttcc
     6721 ttcatctgct tggtaatcag gagcccatca ttgaagtagg ttccgcccag gagaaagtcg
     6781 ggctttctgt tcttggcttc gaggagaatg gaggaaaggt ctttcacatc cttggggtat
     6841 ttcccctcat agacgacctg caggcctctt tccttggcgt aagcggccgc ccccttcccg
     6901 cagtattccg ggaaggcatc atcggcccag atcaccgcgg cggtttttac cggaggattg
     6961 attttctggg acagatcgac caccgggcga aggtagtagg tggccggggg gagaatggag
     7021 ataatgtatt tgaatccccg ttcgtaaatg ttgacggcgt tagcctggca ggccacggtg
     7081 aggacccggt atttctcgcc gatggcgctg gtggccaggg taattccgct gccgtagggg
     7141 cccaggatga atttaacctt gtcttcggtg atcagctttt cggtcagctt ggcgccggtc
     7201 tgggggtcgc tcttgtcgtc atagaagacg atctccaccc tgtaacggtc gggcccgact
     7261 ttgatgccac ccttttgatt caccatctcc atccacaggg tatatccatc cttgacgtac
     7321 cctccttctt tggccaggcg cccggtgatg cataccgaag cccccaactt cagggttttt
     7381 tcggccccga gagccgaccc cgggatccat acgatggcca ggattagagc cgttactgtg
     7441 aaccagacgt tcttcttcat acgttcctcc ttggtgttgg ttggtttcta tctcgttgag
     7501 tcatggttgg gttgtgactc attccgcagg gtttcgtttc cgcccgggtc tcctctggag
     7561 ggcggaatca gatccccagg tacgcttcgc gaattcggtt gtcggcgagc aaccttggcg
     7621 aagtgtcttc ggccacgatc ttgccggttt cgatgatata tccccgtgat gcgagttcga
     7681 aagcgatttg cacatcctgc tccacgagga agatagaaat ttggccggtt tgatggacct
     7741 cccggatggc cgccaccagg cggtccacca tgaccggggc caggcccagg gaaagctcat
     7801 ccaccaggag cagcttgggg tcggccaaca ggccccgggc cagggcgcac atctgctgct
     7861 cccccccgga gagcgtcccg gcctgctggg tccggcgttc tttgagacgg gggaataaat
     7921 tatacaccca ttccagggat ttctggatgc cgtccacgtc cgcgcgggtg taagcgccca
     7981 tgaggatgtt ctcttccacg ctcatccagt aaaagatttc ccggccttcg ggaacgaggg
     8041 agatccctaa ggggactctt ttcgaagggg gcagcgggga aacttccttt ccgaaaaatt
     8101 gaatatttcc cgaaatgggc ttcaagattc cgatgattcc cttcatcagg gtcgacttcc
     8161 cggccccgtt cgatccggca agggcgacca cttccttctc cgcgatgcgc aaatggatat
     8221 cccacaggac ctggatgtcc ccataaccga gattgagact ttgaatttcg agcatcatgt
     8281 ttccgagccc tttcgtgcct tggcatacct ctcccccaga taggcttcga ttacccgggg
     8341 gtctttcacc acttcctgcg gggagccgtc ggcgatcttg cccccgtgat gaaggacggt
     8401 gacgcgatgg caaagcgtca taaccgcttt catgacgtgc tcgatcacca gaaggctgat
     8461 tttcaactcc tcgttgatcc gcctcaccag ggcgatcatt tcctcaatgt ctttggtgtt
     8521 gaggccggcc atgacttcat cgagcatcaa aacctcgggg ttcatggcca gggctttcgc
     8581 caaatccatc cttttccgga aggatatgtt gatctggtcc actccggcgt tgcggtaatc
     8641 ctggaggtgg acgaaatcga tccaccggtc cgcttcccgg aaagcctcct tggtgcttcg
     8701 ccccgctccg tttttaccga agagggcgcc gacgaccacg ttctcccgta gggacattcc
     8761 cgggaagggc tggacgattt ggaacgtgcg ggcgatcccc aggcggctga ccttagccgg
     8821 tttcaaaccg gttatgggcg tccccttgaa ggagatctcc ccctcggtta gcgggtaggt
     8881 tccggttacg agattgacca aagtggtttt cccagccccg ttgggtccga tcaggcccag
     8941 gatttcccct tcccgaacct gaaaatccac cgcgtcaacc gctttgagag ccccaaaagc
     9001 gcgggtgacg tcttttcctt ctaagagagc cataaggtcc tcagacggct tggtttttga
     9061 tgttgttcca gatggcggcc agcgaaaatt tcttgcggaa gaattccatg attccgcggg
     9121 gcatcagaat gacaatggcc accatgatga tccccagaat cagcatgtgg acggtcaaga
     9181 actttcccca gacggtctcg gagatcagtt cgattaaaaa ggcccccaga accgggccga
     9241 gaacggttcc ggcccctccc agcatcatca taacgaaagc cttgaccgcg atcatgatgt
     9301 cgaagaccgt ggccggttcg ataaagctga accagtaagc gaaaacccct ccggtgatcc
     9361 cggtgatgaa ggccgaaatg gcccaggcga caattttgca ccggggggcg aagattccgg
     9421 aggcttcggc tgcgccctca ttttcccgga tggcccggag ggcatatccc agccggcttc
     9481 gggcgatccc gtaattaacc agaacggtga aggccaggag gccgaacatg atgaaataga
     9541 tcattacgta aaaggtgttg atctccattt ggggaagggg cagggtgatc cccttgccgc
     9601 cgccggtgaa gctcccccag tccagcgcaa tttgttccgt cgcccggttg atccccaggg
     9661 ttgcgatggc gaaataatgc ccccggagtc tcaagatggg gatcccgagc agaatggcgt
     9721 aaatggatgc gagcaccccc ccgacaatcg ccccaaccca ccaatacaag ccaacccgga
     9781 gcatgagcat ggcggttgta taggccccga tcccgaaaaa gacgatattg ccgaaagccg
     9841 cgtacccggc aaaaccagcg atgatgttga gggccgcggt gatgatcgcg tacatgaaaa
     9901 tcgaggttat gacccgaatc cagtatcctt ccaggaaaaa gggaaggacg atcaatccca
     9961 gaacggcgat caaaccgaaa atcaaaagaa tctttttctg acctcccacc gaatcttctc
    10021 cttgcttgaa acttggttca aggttcaatt tggtttaaat ttgagggatt taaggcactt
    10081 aaggcatttt tattatttgt atttcgaccc cacgattcct ttggggagga aaatcaggac
    10141 caggatcatg atgatcgcac tgatagcctc ctggaacccc gtgccgaaga tgctggcgcc
    10201 cgtggtttcc accaccccca gcaaaagtcc cccgaatatc gctccccaga cgtttcccaa
    10261 ccctcccaga acgcagatga taaaggcttt catgatgaaa aacccccctt cgaagggggt
    10321 gaaggattgg atggtcgaaa tcagcgaacc gcaggcgccg gccagcgccg cgctgattcc
    10381 caaggtaatg gaatatactt tggccacatc caccccgacg atttgggcgg cctggcggtt
    10441 ctgggaagtc gcccggatgg cgttcccggt ccttgttttg ctcaggaaaa gctggagcag
    10501 ggcggtgaga aggatgcaga taaccaggat ggccaaacgg acatagggaa tgaccaggga
    10561 cccgatctgg aaagaggaac ccgaataaga cggagtgagg gaacgataat caccggtgaa
    10621 aatcatgagc aaggcgttga tgatgaccag gtcgagaccg aaggtgagaa taaggctgag
    10681 aaagatcccg actttgacca ccaaattgag cagatatttc tgcgccccgt acccgataat
    10741 gaaaaggatc gccatggaga tcggcaggct gagaaaggga tccaacccga aagccttgaa
    10801 acaggcgaag gtgacgaagg cgccgacgac gatcatcgtt ccgtgggcta cgttgattac
    10861 gtccatgacg ccccaggcca aagaaaagcc cacggcgata ttcacataga tggcgcccaa
    10921 gaaaattcca ttcaagagga tttgcaggct caaggcacgt tcaccttgtt gcgcttcgcc
    10981 gggaggattg gcgggcaagt cgggttcgcg gaatatccgg aaacaacctc cctcgttttc
    11041 tggaaaaacc agggaagcaa aaaccattct ggaatcagtt atgccatttc ccctcctatg
    11101 tcaagagaaa aatcggagag ccaggcgctc tgccccgagc gaaaatggaa ggggggaaca
    11161 ggtccctatt ttttcttgac aggggggaac ctatgcgagt attctaagct ctatttttga
    11221 taattaattt tcaatatgtg ataatgcaga tgaatcaatc cctagcccat ggcctgcaaa
    11281 ttctgctcct tttcgactct acccgcccgg ccttaaccgt tcccgagatc gtagaacatc
    11341 ttcgcttcag ccagagcaag gcctaccgct tgattcgggc cctggtttac tacgggctcc
    11401 tggaaacgca aaacggaacc gggcagtact ccctgggaat gagtgcattg cgcctgggat
    11461 tcctcgccca gcggaaactc aacctctccg cgctggcctt gccgttcatg aaggaattgt
    11521 cccgcctgac caaggagacg gtcctcctca cggtggtcca ggggacccgg gccatttgcc
    11581 tcgaacgggt ggagagcgag gagcccatcc gctattccct cttcaccccg ggggccagcc
    11641 ttcccctaca cgccggcgct tccagcaaag tactcatggc ctttcttcct gaaagggaat
    11701 ggggccccat cattgcccgt gagggtttac ccaggtttac ccctcagacc ataaccgata
    11761 ggaaaatttt gacgtcccag ctcagggaaa ttcgctgccg aggctatgcc ttcagcgacc
    11821 aggaggtgga ccgggatgtg cgggcggtgg cagcgccgat cctcaatccg cggggggagc
    11881 tgatcgccgg gttgaccgtg gccgggccgg cctatcgcat tcacaaacgg agagtccagt
    11941 ccttgggccg gctggtaagg caatctgcgc agaaaatcgc cggtcgcctc cagggcgaac
    12001 ctcttttgcc cgccccccaa gggaattgta aatttatccg gaagaaatcg attcttatcc
    12061 agaaaggagt ttgaaatggc cgttaacctg aagggcagat cactggccag tttatacgat
    12121 ctgacccgtg aggagctaga gcagattctg aaaacctcgg agcttctgaa gctcgagaac
    12181 tcgcgggggc aggagcatcc gctcctcaag ggcaagaccc tggcgatgat tttcgaaaag
    12241 gcctccaccc gtacccgggt ttccttcgag gtgggaatgt ggcagctggg aggatacgcc
    12301 ctgtacctga gcgcgggcga cctccagctg ggcaggggag aaacgatcgc cgatacggcc
    12361 cgggttctct ccggttatgt gaacgggatc atggcccggg tttttgctca ccagaccatt
    12421 ctcgacttgg tgcgtttttc ccgagttccg gtgatcaacg ggttgtccga ttattcccat
    12481 ccctgccagg gcctcgccga tctattcacc atctacgaga aaaaaggccg ggtttcaggg
    12541 ttgaacgttg cgtatgtggg cgacggaaac aacgtatctc attccctcct gtacggatgc
    12601 tccaaaatgg gagcccacat cgtcatcgca tcgcccaagg gctatgagcc caaggaggag
    12661 gtcatttccc gggccgttga agagggaagg aaaaatgggt ccacggtgaa ggtcacccaa
    12721 gaccctcacg aggcggttcg gggagcggac gtggtctaca ccgacgtgtg gacgagcatg
    12781 ggccaggaaa aggagaagga acagcgcttg aggctcttcc agcctttcca gatcaaccct
    12841 aagcttttgc ggggcgccgg ggaggactac gttttcatgc actgtcttcc ggcccaccgg
    12901 ggcgaggaag taaccgatga agtggccgac tcgaagaaca gcgtgatttt tcaacaggcc
    12961 gaaaaccgga tgcacaccca aaaggccttg ctggccttga ccctgtagaa attgggcttg
    13021 gcggattcga gggggggtgg tctccagttt cttgacctct ggctttccac cggaaaactc
    13081 tcgatccatg acccattaaa ttgcccatgc acccatttaa tggagaggga aaggaaatgg
    13141 ccaaaaaatt cgatgtgctg atccaagggg gactcgtggt ttccggaagc agaatccgcc
    13201 gggcggatgt cggggtccag ggagaaaaga tccttaccgt cgccgaaggc ttatccgcgg
    13261 cggatgccgt gcagaccatc gacgcggcag ggaaatttgt cttcccgggg attcttgatg
    13321 tccacaccca cccggtttac gaagacaaca tccaaggact ttcccggacc gccgcccatg
    13381 gggggaccac gaccctgatc ttttacgcct acgcccgccc ggggatgaaa ttgatcgaca
    13441 ccatcaaagc ctaccgggaa gacggcctga aaaattcttt tctcgatttc gccctccacg
    13501 gagcgatctt cgacgcggcc agccaggtgc cggagattcc caaggctttt gagctgggag
    13561 tcaaatcctt caagatgttt atgacctatg ccaagctgaa atggatgacc gatgattatc
    13621 acctgacctg ggccatggac ctcatctccg aaaacggagg cctggccatg gtccacgcag
    13681 aaagcggtct ggtgaccgat tatatcgagg ataaatccct ccgccggaag gaggatcaga
    13741 aagaagtctt cgtcaaaacc cgcccggatt atttggaagc ggaggcggtg ttccgggcca
    13801 tctccattgc cgcagtcatg cagtgcccga tctacatcgc ccatttgagc tcggccaggg
    13861 gggttttccc cattcagcag gcgcgcggag aaggacaatg ggtgtatgcg gaaacctgcc
    13921 cccagtacct aagccttacg gacgaaattc tgcagaagac cggaccgctg gcgaagatcg
    13981 ggcccccgct tcgttatgaa acggaccgga tggcgctttg ggacgcggtg gaaaagggaa
    14041 ccattgacac tatcgccagc gatcatgccc ccaaagccaa gaggatcacc gattcctttt
    14101 tcgaggcccc cttcggggcc ccttcggcgg aaaccctcct gacggtaacc tacgatgagg
    14161 gggtgaacac tggccggatc ggagcctgta aattggttca gcttttatgc gaaaacccgg
    14221 caaaaatatt cggcttgttc cccaggaaag gaattctgca ggagggggcc gatgcagacg
    14281 tggtggtttt cgacccggtg ctcgcccaca cgatcgaaca acgcacccag cattccggcg
    14341 ccccctacac catgtatgag ggaagaagtt gcttgggcaa accggttttc atcatgcagc
    14401 gagggaaagc cttggtcgat aacggggaga tgaagatgaa gcccggagag gcaaggtttt
    14461 gccccaccaa gattgaaaag gtcaaactgt ggtgaaaata gggagggaga atcgagcagg
    14521 gagaatgaga ggtggtcaag aagatagaac accggggatg aaaaataagg ccagcggcaa
    14581 gtcgggaaag gtattctgta gaattgaggg ctgatcgctg aacgctattt ctgaaggagg
    14641 attcaacatg gccggtctga aggggcaaga cattcttcac gggaaccagt tttccaggaa
    14701 ggacattgaa ggcatcatga aggtggcctc ggcctttgaa aaagaaggga agggggcctt
    14761 gaatgtgctc aaaggaaaga tattagcgac cctgttcttc gaacccagca cccggacccg
    14821 tctttccttc gaggccgcca tgcagaggct gggaggaggg gtgatcagcc tgggatcgct
    14881 ggagagctct tccgtggcca agggggaatc tctgacggac accgcccaga ctgtcgccca
    14941 gtatgccgat gtcatcgttc tccggcaccc ccgccaggga tcggctaagg aggccgcgga
    15001 cgccgtgtcc gttccggtga tcaacgccgg ggacggggcc gggcagcatc ccactcaggc
    15061 gcttctggat atgtacacca tccggaagga gctgggctcc ctcaagaatc tgaaggtctg
    15121 cctggccggc gacctgaaat acggtagaac ggtgcacgcc ctggcagagg tcctctccct
    15181 cttcggcgcc cgcctctatt tcgtttcacc ctccatgctg cgcatgcccg aggagattac
    15241 cgccgggctg cgcagccggg ggatcgaagt gaccgagacc gaggaccttc ttcaggccgc
    15301 ttccctgagc gatttgatct atatgacccg catccagaag gagcgcttca gcgaccaggg
    15361 ggaatacgaa agagtcaagg ggagctacgt gatcaacaat cgattccttt ctcaattgaa
    15421 aaaagagatt accttaatgc accccttgcc gcgggtcgat gagatccatc cggaggtgga
    15481 cgggtatgca ggatcggcct atttccgaca gatgcgcaac ggggttttca tccgaatggc
    15541 gcttctggcc atgatcttcg ggaagcggta aatccatgaa gaagatcgtg atcgccctgg
    15601 gcggaaacgc gatcaaacaa tgggaccaga aagggtctgc ggaggagcag ctggccaatg
    15661 tccggcagag ctgcacccac atcgtcagga tcatccgggg agggaatcgg gtggtcatca
    15721 cccatggaaa cggcccgcag gtgggaagcc tcctcatcca gcaggagagg ggatcgcctg
    15781 ccatccctcc ccagccgctg aatatctgcg gggccatgag ccaggggcag atcggctaca
    15841 tgatccagca ggccctgctg aatgaactga aagacgctcg cctcgaccgt agtgtggtca
    15901 ctctgattac tcaggtcgtg gtcgacggag aggatcccgg gttccgccag cccaccaagc
    15961 cggtcggccc tttctactcc cgtgcccaga aagaaaagta tgaaaagcaa accggcgcag
    16021 cctttcagcg gacgccgatg aagaaagagc tctaccgcag agtggtagcc tctcccgatc
    16081 cactgcggat cctggagggg aatatggtta aggacctctt tgagcggggg gtgatcgtga
    16141 tcgcctcggg gggcggcggg gtcccggtca tcctggatga aaatggacac gtgagcgggg
    16201 ttgaagcggt gattgacaag gacttggccg gggagaaatt ggccgaggcg gtgggggctg
    16261 atatctttat ggtcctgact gatgtagacc gggtttattt gaactacggg aaaaggagcc
    16321 agcggggcct gtccacattg cggctggaaa aggcccagca acacctgaaa aatcatgcct
    16381 ttccctccgg aagcatggaa cccaaggtcg cggcctgtgt gcgtttcatc gaatatgggg
    16441 gagaacgggc catcattacc tccttggatc aagcatccgc tgccctggaa gggagagcgg
    16501 ggacccacct ccttcctgat gaatgaggac cctaacatgc gcctgaacca catcctggaa
    16561 gcccaacagt tcaacaaaaa gatgctcaaa gaggtcttta ccctggccga ccagatggcc
    16621 gccctagccc agggaggggg gagcgacctt ctgaagaatc ggattctcgc cagccttttt
    16681 ttcgtcccca gcaaccggac ccgcttttcc ttcgagtcgg ccatgatccg cctgggggga
    16741 cagatcctcg gttcagacag ggccgaggct ttttcttccg aactcgacgg ggggtccctg
    16801 gaggacacca ttaccaccct taattatttt gccgatgtga tcgtcctccg ccaccacctc
    16861 agcgggtcgg ccaaacgggc cgcgcgggtt tcaaatgttc ccatcatcaa cgccggagac
    16921 ggcacggccc agcaccccac ccaggcgctg ctcgaccttt ataccatcga gcgggagctg
    16981 gggggggtgg atggggtggt gattgccttc gtgggcgacc tggccaacag ccggaacgta
    17041 cgctcgctct gctatttcct ggccaagtat acgggaatca aggttcactt tgtcgccccc
    17101 cagcccctgg gaatgcggga tgacctttgc cagtacctca ggagccaggg ggtcaccttc
    17161 gttgaggcct acgggcccga gccccccctg ctcgacctgg cccggaaggt ggatgtcttc
    17221 tacgtgaccc agattccagt ggggagtttc ggcgaccgac tggacgatta tgaagcctcg
    17281 aaaaggcaat tcgtgatcga ccggcgggtc ctcaaggcca tgaagaaaaa ttccatcatc
    17341 atgcatcccc ttccgcgggg gccggaaatt ccgccggaga tggataagga cctgcgggcg
    17401 gtctatttcc agcagacccg atatggccta tgcattcgga tggcccttct ctgcgcggtt
    17461 tttcaacgat ttccctgagg acggtctctc ttctatccgc ctcgagcctg gccgccccct
    17521 cgaagcttga cagggggaca tcttttttcc tatagtgatg gcaaccacga aaaaccggaa
    17581 gtcctcggtt gtcttatggg caatggggac cggcggtgga aaaattgatt tttcccattc
    17641 ttccccggag ccgttcgatc agccgatggg gcgaagaaga aaagactgag accttagacc
    17701 ggggtgccct tgcgcaggca tccggattcg tcaagcaaaa aggagaagac ccatgaaaaa
    17761 aatcgttttt ggattctgtt ggggggtaat ggggttagct tttctcttgg tgttttcccc
    17821 gcccggagga gcacaggaca agcccctggt catcggggtg gatggaacct ttgcccccca
    17881 tgccatgccc aaaatcggtg ggggggtcga ggggttcaac gtcgatttag ccctcgaaat
    17941 tggccgccgc atgggccgaa aggtagagat tgtttcccag cagttctccg gcctcatccc
    18001 cgggctgaac gccggcaaat tcgattttct ggccgctcca accacggtcc taaagccccg
    18061 ggacaagaat cttcttttca ccgaagggta tctggagacg gactaccagt tcgtcatccc
    18121 catcaaagcg gcggagatca agcagtttga agacctcagg ggaaaagtcg tggcggtcaa
    18181 taaaggctcc aactacgaga tgtggtgtca ggagaacggc cccaaatacg gattcaagac
    18241 cgaatcctac ggaaccaaca ccgatgctgt gcaggcggtt ctggccggac gctcggacac
    18301 caatttggcc gggcacaccg tggccgctta tgcggtcaag aaaaaccccg gccagctgaa
    18361 gctgtcgtac aaaatcaaat ccggcgcggt ctggtccatc cctttccgca aagacgatgt
    18421 gcagatgcgc aaagtagtag aagagacgct ggagtgcatg aaaatggacg ggactgtagc
    18481 caagctggcg gaaaaatggt ttggggtgaa gcctgacaag gactcgcccg cagtcaccgt
    18541 ctatcccggc tacggggtgg aaggattcga agggtacgac cccacccccc atactcctgc
    18601 atgcaaatag ctccccggaa ggatgaaaaa caggcggcgg attcgccgcc tgtttttttg
    18661 gagacagatg aaaaaaggcg aatgaatcca tcggcgccag agcccatttt gaaggtgtcc
    18721 ggtttatgca aatctttcgg tacccttcag gtgctgaagg ggattgacct gcaagtcctg
    18781 ccgcgggaac tcatattcat catcggccca tccgggtctg ggaaaagcac cctgcttcgc
    18841 tgctgcaatc gtttggaaga acccacttcg gggtcgatcc ggattgaagg ggtggacatt
    18901 ctggcacccg gcgtggatat caaccttgtc cgccgccgca tcggcatggt tttccagtct
    18961 ttcaatctct atccccacct gaatgccctc ggaaatatca ccctggctct ccgcaaggtt
    19021 ctgaagaaat ccaaaacgga agccgatgac atcgccttta aggccctggg tcgggtcgga
    19081 ctgagtgaaa aagcccggac cttcccgaac gaattatcgg gcggccagca gcagagggtg
    19141 gctattgccc gggctctggc cctggagccg gcgatcatgc tcttcgatga gccgaccagc
    19201 gccctcgacc cggagttggt aggaagcgtg ctcaacgtga tgcggcagat gcgcgagtca
    19261 ggaatgacca tggtagtggt cagccatgaa atgcgcttcg cccggggcgc ggcagaccgg
    19321 gtgatcttca tggatgaagg gatgatcctg gagcaagggg ccccggaaaa aatcttcaac
    19381 gaacccgatc accctcgcat caaggagttc ttgtcaagcc tggaagcaaa gtgaaatggc
    19441 cttgacccct gcagcccccc gggaatccgg gccggctcag acctcccctg gattctcctg
    19501 gacccgcccg gttggcttgg gtcgggatgc ggacctgaaa ggggacctgt cttgtcatat
    19561 cgaactgttc ggattgaggt cccttcatgc tgaaatcctt ccttttcacc tacttcaacc
    19621 tgccggtcat ggccgaatat ttgccccaaa tcctcgaggg gttcatccta accgtcgagc
    19681 tggctgcggc catcgtggta accggcatcc tggccggttt tatgctggcc gttttgcgat
    19741 cctttcgggt ccggttcctg aattttttca tcgtgatttt tgtcgatgtg ctgcgcgccc
    19801 ttccacccct ggtcattatc atcctcttct atttcgccct ccccagcgtg ggagtccgca
    19861 tgtccggttt tgccgccacc tggctatcct tgagcctggt cctcactgcc tttgccgaag
    19921 agatcttctg ggcggggatc ctggctgttc caaaagggca gtgggaagct gctaggtcca
    19981 cgggcatgtc ttttcttcag accctggggg gggttgtcct tccccaggcg gtgcggatta
    20041 ccatccctcc cttgaccaac cggaccattg cgatcaccaa aggaacggca ctgggcgctg
    20101 tggtggccgt gggggagatc ctgaaccagg ctcagaccgg gctttcccat tcctacaatc
    20161 cctccccgct gaccctggga gcattggcct acctggttct ctttatcccg gtggttaccc
    20221 tggggcgctg ggtggaaacc cgttttgcct ggaagcgatg aaccatggaa caaatcatcc
    20281 aaggcttctt caacatcgaa atcgtaatcg ccgcgtttcc ttttctgctg cgcggtttgg
    20341 ccatgaccgt cctgctttgc ctggtggtca tccccctggg cctgttcgga gggctctcgc
    20401 tggccctggt ttccaccgtc cgctcggtct tggccaaagg gctggttgtg gtttacgtgg
    20461 actttttccg tgcctttcca cccctggttc tcctgatctt catctatttc ggcgtccctt
    20521 tcctgggaat tgacctgcac gccttcaccg ccgtgaccat cggttttttt ctcaatacct
    20581 cgagctatta tggggagatc ttccgggcgg gaatcgaaag catcccgcgg gggcaaagag
    20641 aagcggcacg gtcaacggga ctgacttccg tccagaccat ggtcttcgtc gtaattcccc
    20701 aggcaacccg caacgtgatt cccgacttgg tcagcaatac tctggaggtt gtgaagttga
    20761 cctccatcgc cagcgtcgtg gccttgccgg aattgctcta tgcggcccgg caggcccagt
    20821 ccatcaccta caatgccact cccatcattt ttgcggggat ggtctatctg atttgcttat
    20881 ggcccctggt gcggctcctc aggcgttttg agcataaaaa ggtggccttg cactagtttt
    20941 cctcgggtga catccgagac gggggggaga agaacgggaa gataccggaa gaacctatgc
    21001 tcagggcaga cggccgatga gggtattagg ccctcctgcc tggggaggcg gctttcagaa
    21061 tttcgaggtt taccggattt cgcggttttt ccccgagaag aattcgccgg acttcgaggg
    21121 cggccttggt gcgcatctcc tcgcggctct gctcggtata gaaggcattg tgcggagtca
    21181 ggatcaaatt gaccggcggg tcttcctgct gccataactg aacgatgggg tcctctgccc
    21241 cgggaggctc ctcgggaaga acatccagac ccgcgccccc gattttgccg gccttcaaag
    21301 cttcggccac agccccagcg tccacgcatt ttcccctggc tgtattcacc aggatggcat
    21361 gcggctgcat ccgggtcagc tgcggagccc caacgattcc ggtcgtttcc gggttcaagg
    21421 gggtgtgcac cgaaaccaca tcgctcaccg ccaggagttc gtccagagaa agcagggcca
    21481 cacccagggc tttgtcgtac ccatcagcga ggtagggatc gttggccaca actttcatcc
    21541 ggagggcctt ggcccgcagg gcggtggccg tcccgattct tcccaatccg atgatcccca
    21601 gggtcgaccc ggcaagtctc cggaggggag ttgcggccag ataattccag ggggacaggg
    21661 ttttccgaag ccttctctcg gtaaaggtca gtcccctggc acaggccagc atgagagcaa
    21721 tggcgtggtc ggccacttca tcggttccgt agtcgggaac attgcataca tagatgcctt
    21781 tttcgccggc agcggcgagg tccacattat cgtagcccac cccgc
//
LOCUS       DASPRR010000083         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1795426, whole genome shotgun sequence.
ACCESSION   DASPRR010000083 DASPRR010000000
VERSION     DASPRR010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795426"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1432
                     /gene="hutH"
                     /locus_tag="VEL68_02935"
                     /pseudo
     CDS             30..1432
                     /gene="hutH"
                     /locus_tag="VEL68_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
     gene            complement(1483..2763)
                     /locus_tag="VEL68_02940"
     CDS             complement(1483..2763)
                     /locus_tag="VEL68_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ34956.1"
                     /translation="MTLPCIPQNLRLLNSDGWILFATRTLRLFAYGFLSVVLMIYLAQ
                     IGLSETQIGVLFTLTLIGDTLISLAITTRADRLGRRRMLILGAALMVFAGILFALTRD
                     FVVLLIAATIGVISPSGNEVGPFLSIEQASLSQIVPNERRTQVFAWYNLVGSFATALG
                     ALFGGVLVQVGQDTGLTPIGSYRLVVAGYAAIGMALGLFFTRLSSRVEVSRETSPASI
                     RPKPEGRFGLHQSQKVVFKLSALFSLDAFAGGFVLQSIVAYWFHVRFNVSPAVLGSIF
                     FGANILAGVSALSAAWVASKIGLIKTMVFTHIPSNILLILVPLMPNLPLAIAMLLLRF
                     SISQMDVPTRQSYTMAVVNPEERSAAAGITGIARTTGASLSPVFTGPLLANALLWNVP
                     FFISGSLKIVYDLLLYRSFLALKPPEEKGSPDSK"
     gene            complement(2878..>3418)
                     /locus_tag="VEL68_02945"
     CDS             complement(2878..>3418)
                     /locus_tag="VEL68_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXZ34957.1"
                     /translation="NQAFTRNEAIRVLQALEPFELELAEQPVAWHDLAGMGMVARNVR
                     TPIMAHETLLDRFDALHLVEYGGAGVFGLKLDRPGGFVNVKKVNALAELNNIPSFMCS
                     SIELGISTSAAAHLAVSQKNIKLASEFSGPQTISDDIVKNPIPIKDGYAKPWDAPGLG
                     VELDEKKLAKYAGKAIVCE"
BASE COUNT          835 a          914 c         1001 g          668 t
ORIGIN      
        1 gtgagaaggt gaaacggtcc agggattttg tggaaaaggc tctccgccaa ggggagaaga
       61 tttacggggt gaccactgga tttggacttc ttagcgatca actgattaac ccgaaggaga
      121 ttcaagaatt gcaaaggaat ctcattcgca gccattccgt gggggtagga cctttcttcg
      181 atgaagcgac gacccgggcg gtcatggtcc tccgagcgaa cgtgattgcc aaaggctatt
      241 ccggggtacg aatggaagtg ctaagacgat tgggtgacat gatcaataag ggagtccacc
      301 cgctgattcc ggagcaaggg tcggtggggg ccagcgggga cctggccccc tggcccatct
      361 gagctccgtc cttatgggtg agggagaagc gatctaccgg ggaaaaagga tgccggggaa
      421 aaaggccatg gaaaaggccg ggattccgct cgtcaccttg aaggccaaag aagggttgtc
      481 tttgatcaac gggacccagg tcatgactgc cgtcgggctt ttggcccttc ttcgggccga
      541 gcggctttgc aaggtggctg atatcgtagg agcttgcacg ctggatgcgc taaagggaac
      601 cttctctgct tttgacccgg acatacagag ggttcgcccc tttcccggtc agctggcggt
      661 ggccagaaac ttccagaagc tcggaaaata cgatcccatc gccgagtcac acaagtcctg
      721 ccccaaggtc caggatgcct actccattcg ctgcatgccc caggtccacg gcgcggtgag
      781 agatgctctg ggttacgtcc gtaggacgct ggaggtggaa gttaattcgg ccaccgataa
      841 tcccctcatt tttgccgagc gggggaagat cctcaattgc ggaaacttcc atggggagcc
      901 agttgctttc gccctcgacc tcctggggat cgcaatagcc gagctggggg gcatttccga
      961 gagacgaatc gagaaattaa tcaaccccgc cttatccggc cttccgcctt tcctgaccca
     1021 aaagggcggc cttcactcgg ggttaatgat ggtccaggta agcgccgcat ccctcgtttc
     1081 ggagaataag gtcctggctc acccggccag cgtggattcc attcccactt cggcggataa
     1141 ggaagaccac gtttccatgg gaaccatcgc cgcgagaaaa gctagggata ttgtcaggaa
     1201 cgtggagcag gtcctggcca tggaactcct ctgcgcgacc caggggctgg aatttctgct
     1261 tcccctccat cccggaaggg ggatctatga agcctacaag gtcgtccggg aaaaagtccc
     1321 tcccatccaa ggcgaccgca ggttcagcaa ggacatccaa aaaatccatt ctttgattga
     1381 atccggagaa ctgcttcgca gcgtggaaaa gatagccggg aatctaaatt gaagggaacc
     1441 ccgctgggag ggcagggctt gagccgattt ccgcaccctt ttttattttg aatcggggga
     1501 tcctttctct tcggggggtt tgagggccag gaaactgcga tacaagagaa gatcatagac
     1561 gatcttgagg ctgcccgaaa tgaaaaaggg cacattccac aaaagagcat tggccagcaa
     1621 gggtccggtg aagacagggg acagagaagc tccggtggtc ctggcaatac ccgttattcc
     1681 cgcggccgcc gagcgttcct ccggatttac tacggccata gtataagatt gacgggtcgg
     1741 aacatccatc tgggaaatgc tgaaacgtaa cagcaacatt gcaatggcca agggcaggtt
     1801 gggcatgaga ggaaccagga ttagcaggat attagaggga atgtgggtga ataccatggt
     1861 tttaatcaac ccgatcttgg aggccaccca tgcggcgctc agagctgaaa cccccgcaag
     1921 aatgttggcc ccgaaaaata tgctgccgag caccgcaggg gatacattga aacgcacgtg
     1981 aaaccagtaa gctacaatgc tctgcaggac gaagccgccg gcaaaggcat caagggaaaa
     2041 cagggctgaa agcttgaaca ccaccttctg cgattgatgc aacccaaaac gcccttctgg
     2101 ttttgggcga atcgaggccg gggaggtctc ccgggaaact tcaactctgg aggaaagcct
     2161 ggtgaaaaac aacccgaggg ccatcccaat ggcggcataa cctgccacca ctaaccggta
     2221 gctcccgatg ggggttaagc cggtatcttg tcccacctgc accagcaccc cgccgaataa
     2281 ggcccccagc gccgtggcaa acgatcccac cagattgtac caggcaaaaa cctgggtccg
     2341 ccgctcattg ggaacgatct gcgacaaaga ggcttgctcg atggacagga agggacccac
     2401 ctcattcccg ctggggctga tgactccgat cgttgccgcg atcaataaaa caacgaaatc
     2461 gcgcgtcagg gcgaataata tcccggcgaa aaccatcagg gccgcaccca agatcaacat
     2521 ccgccttcgg cccagccgat cggcccgggt ggtgatcgcc aaggaaatga gcgtatcgcc
     2581 gatcagggta agggtgaaca agacgccgat ctgggtttcg ctcaggccta tttgagccag
     2641 gtagatcatg agcaccacgg aaaggaaccc gtaggcaaaa agacgaaggg tgcgggtcgc
     2701 gaagagaatc cagccgtcag aattcaacaa tcgcagattt tgaggaatgc agggtagagt
     2761 catgaagggc ttcttccatt tcagaaatcg aaggactgat ttggggaaag cgcgagacca
     2821 taggggaaat aagaggcgta agggcgaccc gccaggtcgc ccctacgaat ttgaatatca
     2881 ttcacaaacg atggctttcc ccgcatattt ggccagcttt ttctcatcca actcgactcc
     2941 taaaccagga gcgtcccagg gtttggcata gccatccttg atcggaatcg ggttcttgac
     3001 gatatcatcg gaaatggttt gcggtccgga gaattcgctg gccagtttga tgtttttctg
     3061 ggaaacggct aaatgagcgg cggcggaagt ggagattccc agttcgatgg aagagcacat
     3121 gaaactgggg atgttattca actctgccag ggcattcacc ttcttgacat ttacaaatcc
     3181 cccgggccgg tccagcttca ggccgaagac cccggccccg ccgtattcga ccaggtgaag
     3241 ggcgtcgaaa cggtcgagca gagtctcatg ggccatgatc ggcgtgcgca cattgcgggc
     3301 caccatcccc atgccggcca ggtcgtgcca cgccaccggc tgttcggcca gctccagctc
     3361 gaacggctcc agggcctgga gcacccggat cgcttcattg cgggtgaacg cctggttg
//
LOCUS       DASPRR010000084         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_986349, whole genome shotgun sequence.
ACCESSION   DASPRR010000084 DASPRR010000000
VERSION     DASPRR010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986349"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1237)
                     /locus_tag="VEL68_02950"
     CDS             complement(248..1237)
                     /locus_tag="VEL68_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HXZ34958.1"
                     /translation="MERKTLILTASEVLSCLDPDEVFRVVEETFRDAGLGRTILPPKL
                     FMYLPGEGHKDFMFAGPASVPSAKAVGVKIVTGYKNNPEKFNLPHILAVMVLTDPENG
                     YPLALFDGTHLTTFRTGAMIAVAAKYLARSDSEILAIIGAGTQGRSAAKALSRTFSFR
                     EIHLQDINSRAGEEFGEEIGPLLKLPIRLSSQAKEAVEGADIIVTVTSSEGEFVKDEW
                     IKPGSFIASVGSYPELEPALPKSVEKLVVDDRTQARYRGELWKGYERGEFTDSMIYAE
                     LGEVVCGRKKGRETRNERTLACLTGVGTLDVAVARQVYERALKRKLGLWVSLW"
     gene            complement(1457..2815)
                     /locus_tag="VEL68_02955"
     CDS             complement(1457..2815)
                     /locus_tag="VEL68_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ34959.1"
                     /translation="MAKPTSVERYETKFPRSKEMFTKAKGLVPRGVAHDAWQASPFPL
                     FMTQARGSHIWDVDENEFIDYFGGHGGKVLGHAHPAVVKAAKEQMEKGVQFGTNCDLM
                     LEWAGIIQKLVPSAERIEFTNSGTEAIMLGVRLARAYTGRKKIVRFQFHFAGAYDAVT
                     VGYKKPFQVSVSGGILPSAVEDTIVLPMNEEAVLEGTLQNRDVAVVMVEAAGASSGVV
                     GIKPSFYGTLRNLTQKYGALLLFDEIVTGFRYSPGGVQAAVGVTPDLTTLGKGITGVM
                     PGAGAIVGKRNVMEMFLFKDEDWNRYKRVSHFGTFNANPLCAATGIAYLNIIATGQPT
                     GEATRNARKLRDGMQRKLDEREIPGCVYDSNFSVVHMYLGKCDLQGSCDRVVCVNALK
                     DRPSDVGEALYRSFALHGVKTAARGFDLYVSAVHSEEDLERTIKAFGNTLDTVIEEKT
                     LK"
     gene            complement(3075..>3444)
                     /locus_tag="VEL68_02960"
     CDS             complement(3075..>3444)
                     /locus_tag="VEL68_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HXZ34960.1"
                     /translation="IIQVHSGKEYLIYDTGFIGGSAHFKVPPPLDSLPRKKTPNLGVP
                     AGAVLIAGGLGSVYKPLAGPTGWYWIGMSPLKQWFPEKDPPLLIHPGDKVIYRPVDKE
                     EFDRIKKKVEEGNFTAKILS"
BASE COUNT          792 a          982 c          829 g          841 t
ORIGIN      
        1 ggggaatcat ctccatcacg ccgaacgtca gaaagacaag cgctaggaaa acgatccttc
       61 tccccatagg gacctcctga atcaagatca aattcaaatc atgctaactt ttctgatcag
      121 gggtgtcaag gttaagggaa agccccagcc attgccgatt tcggattgcg gatttcggga
      181 tttatgctgg ttttctttct ttcaatccgc aatccacaat cgcaaatccg catttgaatg
      241 ggtgggttta ccaaaggctg acccagagcc ccaatttgcg tttcaaggcc ctttcgtaca
      301 cttgccgggc aacggccacg tccagggttc cgaccccggt cagacaggcc agagtcctct
      361 cattcctcgt ttccctgcct ttcttccttc cgcatacaac ctcaccgagt tcggcataaa
      421 tcatcgagtc ggtgaattct cccctttcat atcctttcca tagttccccc cggtagcggg
      481 cctgggtccg gtcgtcgacc accaactttt cgacactttt cggcaacgcc ggttccaatt
      541 caggatagga gcccacgctg gcgatgaaag accccggttt gatccattca tccttgacga
      601 actcgccttc agaggaggta accgtgacga tgatatccgc tccttccacg gcctctttcg
      661 cttgagagga aagccgaatg gggagcttca gcagaggtcc tatttcctcc ccgaattctt
      721 ccccggcccg ggaattgatg tcctggagat gtatttcccg aaaggaaaag gttcggctga
      781 gagctttagc cgcagacctc ccctgggtcc cggcccctat aatcgccaga atctccgaat
      841 ccgaacgggc aaggtatttg gcagcgactg caatcatggc gccggtccga aaagtggtca
      901 ggtgagtccc gtcaaataga gccaggggat acccgttttc tggatcggtc aggaccatga
      961 ccgcaaggat gtggggtaag ttaaactttt ccggattgtt tttgtagcca gtgacgatct
     1021 tgacccctac cgctttagcc gaagggacac ttgccgggcc ggcaaacata aagtccttat
     1081 ggccttcccc gggtaagtac atgaacaact tcggggggag aattgtccga ccaagaccgg
     1141 catcccggaa ggtctcttcg acaacccgaa agacttcatc tgggtcgaga cagcttagga
     1201 cctcggaagc ggtgaggatc agggtttttc tttccataaa tcctaacccg gcacagccgg
     1261 aaacaaacag gtctgacgaa ttcaaaaatc cgaaacaaat tcgaaattca aaaaaacaaa
     1321 ctaagaaaag acttttgaaa tcggaacatt tgcatttgat ccgaatttcg ggtttcgtgc
     1381 tccgaatttg gaaccagcgc tttccattgg aaatttcctt ccatcttgcg cgcaacttga
     1441 ctcttgaaag actatactat ttcaaggtct tttcttcgat cactgtgtcc agggtgtttc
     1501 caaaggcctt gattgttctt tccaggtcct cttcggaatg gacagcggac acatataggt
     1561 caaaacctcg cgcggcggtc ttcactccat ggagggcgaa actccggtag agggcttccc
     1621 cgacgtccga tggacgatct tttagggcat tcacacacac cacccggtcg catgaccctt
     1681 ggagatcgca tttccccaga tacatatgga ccacggaaaa attgctgtcg tatacgcacc
     1741 ccgggatttc tctttcatcc agttttctct gcatcccgtc cctcagcttt cgggcgtttc
     1801 gggtggcctc ccccgtcggc tgtccggtgg cgatgatgtt cagataagca atgcccgtgg
     1861 cggcgcagag gggattggcg ttgaaggtgc cgaaatggga aacccgcttg tagcggttcc
     1921 agtcttcgtc cttgaagaga aacatctcca tgacgttcct ctttcccaca atggccccgg
     1981 ctcccggcat aaccccggtg ataccctttc ccagagtggt taagtcagga gtcaccccca
     2041 ctgcggcctg cactccgcct ggggaatagc ggaagccggt cacgatttcg tcaaacaata
     2101 gcaaggcccc atatttttgc gtcaaattgc gtagagtccc ataaaaggaa ggtttgatgc
     2161 ccacaacccc cgaactggcc ccggcagcct ccaccatcac caccgccaca tcccggttct
     2221 gcagggttcc ctccaggact gcttcctcgt tcatcggaag cacgatcgtg tcttccaccg
     2281 ccgaagggag aattccgcct gacaccgata cctggaaagg cttcttgtag cccaccgtga
     2341 ccgcatcgta agccccggca aagtgaaact ggaaccggac gatctttttc cttccggtgt
     2401 aggcccgggc gaggcggact cccagcatga tagcttccgt tccggagttg gtaaactcga
     2461 tacgctcagc cgagggaacc agcttctgga tgattcccgc ccattccagc atgaggtcgc
     2521 agttggtgcc gaattgaact cccttttcca tctgctcctt tgccgctttg accacagctg
     2581 ggtgggcatg gccgagcact ttccccccgt gacccccgaa gtagtcgatg aactcgttct
     2641 cgtccacgtc ccagatgtgg gaacccctgg cttgggtcat gaaaagagga aagggcgatg
     2701 cctgccaggc atcgtgggcc accccgcgag gaaccaatcc cttcgctttg gtaaacatct
     2761 ccttcgatcg gggaaatttc gtttcatagc gctctacgct tgtcggctta gccattcttt
     2821 tactcccttc ataattttcg gtagcaaccc tttaatcgtt ggtatatccg actacagcct
     2881 catgcacggt attatcattg caaacaaagt aaagcaatcc aattcaattg aaaaatcaat
     2941 cagattgcca cggcgctttt cgcctcgcaa tgactggatg aaaaaaattc tcaaaagcat
     3001 aatatatcaa cgattatagg gatactactt gattttcaat atttcttttt atccgcttga
     3061 attttcggtg cccttcagga caggatttta gccgtaaaat taccctcctc caccttcttc
     3121 ttaatccgat cgaactcctc tttgtcgacg gggcggtaga tgaccttatc ccctggatga
     3181 atcaagagag gaggatcttt ttccgggaac cattgcttaa gaggagacat gccgatccaa
     3241 taccagccgg tgggaccggc caggggttta tagacgcttc ccagtccccc ggcgatcaaa
     3301 accgcccccg cgggaacacc caggttggga gtttttttcc gcggcagcga atccaggggt
     3361 ggcggcacct tgaaatgggc cgatccgccg ataaatccgg tatcatagat tagatactct
     3421 ttgccgctgt gaacctggat gatc
//
LOCUS       DASPRR010000085         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_824550, whole genome shotgun sequence.
ACCESSION   DASPRR010000085 DASPRR010000000
VERSION     DASPRR010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824550"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(441..2510)
                     /locus_tag="VEL68_02965"
     CDS             complement(441..2510)
                     /locus_tag="VEL68_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HXZ34961.1"
                     /translation="MVKLWRFNLKKRSIGIHAADLSMIDIQEINKQYGEKVLFSSCSL
                     RLGARERMGLVGPNGSGKTTLFRMIYGEELPDRGEILLRRGTHTGFLSQEPAPVKGLS
                     LLEEVQGGAKGLALLEDKMHLLQEEIAEEKDPQVLEALAQAYGQLEERYARQGGYTLE
                     SQAKAILLGLGFKEEDFIRSSEELSGGWLMRLSLAKILLASPDLLLLDEPTNHLDLES
                     LIWLERFLADYLGSVMMVSHDRDFLNRVAKKIAAIEERKIVLYSGNYDEYLRLREERA
                     ALREAAIENQRRKIEQTGKFIDRFRYKATKARQVQSRIKSLERLEQIEIGETQKTIRF
                     SFPQPARSGRVVAALKGVHKAYGPVKVYSGIDLTIDRGEKIALVGVNGAGKSTLLKLL
                     AGVFPPDEGTLELGHNVATAYFAQHQLELLDPSKTVWEEVFSLAKDEPVSFLRGLLGA
                     FLFSGEEIEKKVSVLSGGEKSRLVLAKMLMHPANFLLLDEPSNHLDMAAREVLQNALQ
                     EFEGTLCLITHDRHLMNAIANRVIEVNNGRLSPYLGNYDDYLYKKGLEKEAAEKEIPG
                     KSSVQETAGREPLGRKSKEQRRYEAEVRNRFFRQTQALRQRTREIESALDQAAQDMQA
                     IEAKLADPDIYRKGEKIADLVKVHSDLKKRTEALTSEWDTLSLQLQEMEQQREAQLEN
                     LDSRANS"
     gene            2635..2826
                     /locus_tag="VEL68_02970"
                     /pseudo
     CDS             2635..2826
                     /locus_tag="VEL68_02970"
                     /inference="COORDINATES: protein motif:HMM:NF002571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
     gene            complement(2937..4358)
                     /locus_tag="VEL68_02975"
     CDS             complement(2937..4358)
                     /locus_tag="VEL68_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ34962.1"
                     /translation="METEKRMLFEKMFHPRSVAIVGISRTYTGLGGQFFLKNLQRAEF
                     PGKVYLINPTAGEVSGMKAYPSISALPEAVDLAIICVPAQFVPPVLEECGRKGVRNIH
                     ILSSGFKELGTSEGIRLEEEIRRVAIQGGLKVIGPNCMGPYVPKSRLMPWGQIPGTAG
                     SMAFLSQSGTLTQRISEHGHFTGVGISKAVSFGNAAVLDSSDYLEYLAEDDETRVVGL
                     YLESVRDGRRFLEVAQWVNQTKPLVLWKGGETSSGAGAVASHTGTLAGEDRVWENGLR
                     QAGITRARSLEEVVGTAMAFLYLPPPKGRRLFILGGGGGNSVYYADICMRVGLQVLPL
                     SGGTRDQISAMVPSVGSFARNPVDAWRAFYDPDYMKQILEVVFEEPDLDMIILDRLIP
                     RMTYAVPEGKNASQMAIDYLQRNRHRKSLVAVVDGAGEDPSLAGEAAMLRQRYCRAGI
                     PAYSSLPLAAQALARVAAYYERK"
BASE COUNT         1025 a         1331 c         1305 g         1112 t
ORIGIN      
        1 ttcctgccca tgcccaaaga ggaaggcggt ccttccgggg ctggaaaagg attccttctg
       61 ctctccgggc gaaaggaaag agaaagcgat cttccagaat ggggaaaccc attaggtttt
      121 ccgaaaattc cggctgatca aaagctcctg aattcttttc ctgggcgcaa aaatgccgaa
      181 ttaagagaat gttcccgggg ttctgaagta ccagagccgg atcacggggc tgaagatcag
      241 atagctgtgg tattttttct aaccagaaag ctcctctcaa aaaccgcagg gcccttggat
      301 tcccgtttga ttcgagatga taatcggctg gaaagggcct tgggaaaaat tcacccgcgg
      361 ttacccatct ccattcgtct catcctgcgg aagaaaaggt atattcagtt tatgctcgac
      421 aaccgggagc gtttaatccc ctaagaattt gcccttgaat ccaggttttc tagctgggct
      481 tcccgctgct gctccatttc ctgcaattga agggacaggg tgtcccactc cgaagtgagc
      541 gcttcggtcc tcttttttaa atcgctgtgg accttgacca agtcagcgat tttctccccc
      601 ttgcggtaaa tatcaggatc agccagtttg gcctcgatgg cctgcatatc ctgggccgcc
      661 tgatccagtg cggattcaat ctcccgggtc ctctggcgaa gggcctgagt ctgccggaag
      721 aaccggtttc ggacctcggc ttcatatcgt ctttgctctt tgctcttccg accgagaggc
      781 tctctgccag ctgtctcctg gaccgaagat tttccgggga tttctttctc agcggcttct
      841 ttttccagtc cttttttata gagatagtca tcatagtttc cgagataagg agacagacgc
      901 ccgttgttga cctcgatgac tctattggcg atggcgttca tcaagtgacg gtcatgggta
      961 atcaagcaga gagttccctc gaattcctgg agggcgttct gcaggacttc ccgggcggcc
     1021 atatccaggt ggttgctggg ttcgtccagg agaagaaaat tcgccggatg cattagcatc
     1081 ttggccagga ccaaccggct tttctccccg ccgctcagga cggacacttt tttttcgatc
     1141 tcctcccccg aaaaaagaaa ggcccccagc aggccgcgca aaaaactgac cggctcgtcc
     1201 ttggccaagg aaaagacctc ttcccaaacc gtcttggagg ggtccagaag ctccagctgg
     1261 tgctgagcaa agtatgccgt ggccacattg tgtcccaatt cgagcgtacc ctcgtcgggg
     1321 ggaaacacgc cggccaggag tttcaggagg gttgattttc ccgccccatt taccccgacc
     1381 agggcgattt tctccccccg gtcgatagtc agatcgatcc cggaataaac tttcaccggc
     1441 ccgtaagcct tgtggacccc tttcaaagcg gccaccaccc ggccgcttcg ggctggctgg
     1501 gggaaggaaa aacggatcgt cttctgggtt tccccgattt cgatctgctc cagcctttcc
     1561 aggctcttga tccggctttg cacctggcga gctttggtcg ccttgtagcg gaatcggtcg
     1621 atgaactttc cggtctgctc gatcttgcgg cgctggtttt ctatggcggc ctcgcggagg
     1681 gccgctcttt cttcccggag ccgcaggtac tcgtcgtagt ttccggaata gagtacgatt
     1741 ttcctttctt caatggccgc gatcttcttc gccacccggt tcaggaaatc tcggtcgtgg
     1801 gagaccatca tgaccgaacc caggtagtcc gccaggaacc gctccagcca gatgagggat
     1861 tccaggtcca gatggtttgt gggttcgtcc aggagaagga ggtcggggct ggcgaggagg
     1921 atcttggcca aagacaggcg catcaaccat cccccgctga gctcttctga ggaccggata
     1981 aaatcctctt ctttgaaccc cagtcctagc aaaatagcct tggcttgaga ctctaacgtg
     2041 tatcctccct gcctggcgta tctttcctcc agttgcccgt aagcctgagc tagcgcctcc
     2101 agcacctggg ggtccttctc ctcggcaatc tcttcctgga ggaggtgcat cttgtcttcc
     2161 aggagagcca ggcccttcgc cccgccctgg acctcttcca gaagggaaag gcctttcacc
     2221 ggcgctggtt cttgagagag aaaaccggtg tgcgtccccc tgcggagaag gatctctccc
     2281 cggtcgggaa gctcttcgcc gtaaatcatc cggaagagag tggttttccc cgacccgttc
     2341 ggaccgacca ggcccattcg ttcccgggcc cccagccgga gcgagcagga agagaagagg
     2401 accttctccc cgtattgttt gttgatttcc tggatgtcga tcatggaaag gtctgccgca
     2461 tggatgccga tggacctctt cttgaggttg aacctccata attttaccat ctcctgccaa
     2521 aagggagccc ccttttttta atttttgaga gcgattcgtg tttgcatccc acttgcgcat
     2581 ccttataatg gtctaagggg ttgctagccc gtaagggaaa aaagaaagga gagcgagatg
     2641 cccatcattc acgtgcacat gttcgaaggg agaacggtgg atcagaagag aaaactcgtc
     2701 gccgccatga ccgatgcggt ggtgaagacc ctggatacca aaccggagac ggttcgcatc
     2761 ctgctccacg acatgtccaa gaacgacatg tccgtggccg gggtgctcca ttcggacaaa
     2821 aaatgacagc cgaaaactcc ctctcccctc gcgggagatg gttagggtga ggggggagag
     2881 aaaaaaggag tcttcggatg aaggccagct gatagctgac cgctgaaagc taaaagctac
     2941 ttcctttcat aatacgccgc cacccgcgca agggcctggg ctgctagagg aagggaagag
     3001 taggcgggga ttccagcccg gcagtagcgc tggcgcagca tagctgcttc tccggcgagg
     3061 ctgggatctt cgccggcgcc gtcaacgacg gcgacgaggg atttacggtg gcgatttctt
     3121 tggaggtagt cgattgccat ctgggaggca ttcttccctt cggggacggc gtaagtcatc
     3181 cgagggatta gccggtccag gatgatcata tccaggtccg gttcctcgaa tacgacctcc
     3241 aggatttgtt tcatgtagtc gggatcataa aaagccctcc aggcatcgac gggatttcgg
     3301 gcgaaagaac ccaccgaagg gaccatggcc gaaatttgat cccgtgttcc tccgctcaag
     3361 gggaggacct gcaatccaac ccgcatgcag atgtccgcat agtaaacgct gttgcccccg
     3421 cctccgccca ggatgaaaag acgccggccc ttgggtggag gaagatagag aaaagccatg
     3481 gcggttccca cgacctcctc cagggaacgg gcgcgggtga ttccggcttg tcgaaggccg
     3541 ttttcccaga ctcgatcctc tcctgccagg gtaccggtgt gtgaagccac tgccccggct
     3601 ccggaagacg tttcccctcc tttccaaagg accagaggtt tggtttgatt gacccactgg
     3661 gctacttcga ggaagcgccg tccatcccgg acgctctcca gatataagcc taccactctc
     3721 gtttcatcgt cctcggccag gtactccagg taatccgagc tgtccaagac cgccgcgttc
     3781 ccaaaactga ccgccttgct gattcccacg ccggtgaagt gtccgtgctc gctgattcgc
     3841 tgggttaagg tcccgctctg ggacaaaaag gccatggaac cggcagtccc aggtatctgt
     3901 ccccagggca tgagccgaga tttgggcaca tagggaccca tgcaattagg accgatgact
     3961 ttcagcccgc cttggatggc tacccgtcgg atttcttctt ccagccggat tccctcggag
     4021 gttcctaact ccttgaaccc ggaactcagg atgtgtatgt tgcgcactcc cttacgacca
     4081 cattcttcca ggaccggggg cacgaattgg gccggtacac agatgatggc caggtccacc
     4141 gcttccggaa gggccgagat gctcggatag gccttcattc cggagacctc ccctgcggtg
     4201 ggatttatga gatagacctt cccgggaaac tcggcccgct gcaggttctt gagaaaaaac
     4261 tggcccccca gcccggtgta agtcctggag attccaacga tggcgacgct tcgagggtga
     4321 aacatctttt caaaaagcat tctcttttcc gtttccatta gcccttcttt ttcccttcca
     4381 atccgatcgc tgccatgccc ttttcgggaa tggattaagg tttaaaactc ccaactcgaa
     4441 acttgaaaat tgctttaagg agtcattata acgaaaaaga tcaaaaaagt tgactccagg
     4501 tttagatttc ctttatatta gacctgcctg aaatcagtga aagcgaagag aactgcgaga
     4561 ggcaagaaac agagggcagc acagagggga tttctgatcc tcttagttct cgaaggttca
     4621 tatttttgct tcggaaagga gtttggaaat gcgagaagtg gtcatcgtcg gaatggttcg
     4681 cacggccatc ggccgcatgg gagggacgat cgcgcggtat cggccggaag agctaggagc
     4741 ctttgttatt cgaggaatcg tggacaagac cgg
//
LOCUS       DASPRR010000086         8080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1796207, whole genome shotgun sequence.
ACCESSION   DASPRR010000086 DASPRR010000000
VERSION     DASPRR010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796207"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(708..893)
                     /locus_tag="VEL68_02980"
     CDS             complement(708..893)
                     /locus_tag="VEL68_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXZ34963.1"
                     /translation="MYQVTVDKDKCDGKGECVDTCPVSVFEMKDGKSEPVNMDECLGC
                     ESCVEVCPTGAITVKEV"
     gene            1087..1938
                     /locus_tag="VEL68_02985"
     CDS             1087..1938
                     /locus_tag="VEL68_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34964.1"
                     /translation="MMTRREEEYIGENAYLPEHVIPLMVGISQAEPFFQEDHLFFLKD
                     DWMIFIGYPLGREFQEEAFSRVLEKMQERFQPATTWFIAPGIPESLLPQIRGKEVDEY
                     YRLDLRQSGMKRSLQREVEKAALVLRVEENRDWSPEHESLTREFLKNEDLSPRVRELY
                     LRMPQVLAHSETSLALSARDSKGNLAAFFVIELGARRFATYVVGCYSQSHYVSHASDL
                     LFQEMIRIARERNKEYIHLGLGVNEGIRRFKKKWGGVASLPYFAGEIAAREKVALSWL
                     RALASKI"
     gene            1935..2795
                     /locus_tag="VEL68_02990"
     CDS             1935..2795
                     /locus_tag="VEL68_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HXZ34965.1"
                     /translation="MNLNDRMKYFIFPGEKQLKTVWEAEKNGKKNYIVGTAHFFPYHF
                     RISLGKILRRSKRALFEGPLDPQSMEKVVTHGSQGNGSMEIYQALDFQALKEIRGKAA
                     DLFGDSHPLLQFLPGGQKPLDPLQAHFLKLRPWLAFFNIWTFYLKSKGWTGSVDLEAF
                     DLAKKLGREIQFLETIEEQIQVLEQIPVDRMIRFLKKVHHWDEYSESYVRMFLQGALA
                     DWMSGTSDFPSRCSPVIGERDRVFYCRMKPFIEKGESAVFLGAPHLHGVNQMLEADGY
                     RVEQTREIEE"
     gene            2792..3640
                     /locus_tag="VEL68_02995"
     CDS             2792..3640
                     /locus_tag="VEL68_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34966.1"
                     /translation="MITPQEEDFLTLHAYVPEHLPGYGRVFSGGGEPFLVGSYLGYLR
                     EETLIWVGYPLEGAFLEEEMKSALETALKKYRVSRVAITASSLSGMGGGKKTVDFYYR
                     LELTSLRVASKTKNMVHRAGRELTIDKGPEFGGDHQALIDLFLGSRELEEGTRIIFSR
                     IADYLSSVPAARVFAARDSRGTLAGFDVADFGAGEYAFYLFNFRSQEAAVPGTSDLLL
                     HALIREAQERGKRYLNLGLGISEGVAFFKKKWGGRPFLPHKSLIYRPSPPSIFDFLMK
                     GLTQVC"
     gene            3634..4734
                     /locus_tag="VEL68_03000"
     CDS             3634..4734
                     /locus_tag="VEL68_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ34967.1"
                     /translation="MLKGLWKKIFPASPRKKFAAWQVELTSRCPLQCKMCSRTAYKDF
                     FSRDMSLDDFQKLLPYFPEVEAVVLEGWGESLLHKNLIECIGWVKEKGPRAGFVTSGK
                     GLNESLICELLKVGVDFMGFSLSGATPETHNAIRVNSDLDDLKKNILTLQEMKARQNL
                     ANPHLHIVYLMLKENICEVPVLIQLARDLGIKQVILIHLIQVSNSWQEEQRVFGENGS
                     EQFENILQEAQAKAREWKIELQCPSLSSGEVPICAEKPLRNLYISVDGEVSPCVYLNP
                     PVPTPFKRIFQKKEFPIEKVSFGNIFQKPFPEIWQSPQYEEFRGCFTRRQEEMQKMIG
                     DLWDPGKRKDLESYSLPPPPVPCRTCYKIEGF"
     gene            complement(4878..5720)
                     /locus_tag="VEL68_03005"
     CDS             complement(4878..5720)
                     /locus_tag="VEL68_03005"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXZ34968.1"
                     /translation="MEIKNWIEVIGKFMRDEKFELPLPGPTYQKVQRFMGEKNLDIRE
                     VEKLINYDQSLTIRVLRMANSAFYRGWQKVATVREAIMRLGTNEVMNILLLIAQRENF
                     SAQDPWVSKVMHKLWEHSVASAVGAQWIARNCRFRSLQQEAFVAGLLHDVGMSFLMTV
                     IEKIKLKGGSGLPLDEEAIRQVLNVLHTENGYSLLNKWHLPEAYCSVARNHHREEIDP
                     NDELLVIVRLANLACHKMEIGLMAEPSLILGSRAEVTWLAFSEEALTRLQAKVEEAVL
                     LTSL"
     gene            6209..7918
                     /locus_tag="VEL68_03010"
     CDS             6209..7918
                     /locus_tag="VEL68_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ34969.1"
                     /translation="MQSERMKVVIVGGVAVGPKTAARLRRLNPEAEITILEKGKVLSY
                     AGCGMPYYVSGDVPDSKNLMDTPAGVIRDSLFFLNVKGITVLDRTLAEKIDRKAKTVS
                     VVHLETGEHKALPYDKLVLATGGSPIELPIPGKELNHVFRMWQPEDALAMRSFILEKK
                     PRRGVIIGGGLIGIEMTEALVKQGLKVSVVEMLPFILPGLLDVEVATYLNRYLRMKGV
                     DVRCSTRVTRIEGDDQGNVQKVITEKGDIPADLVLISVGVRPNSKLAKEAGLEVGEMG
                     DIRANEYLQTNDPDIYAGGDCVQNIHLLTGKAVFAPMGSTANKHGRLIANNIMGYGER
                     FKGILSTAVVKVFDYNVGKSGLTESQAREAGFEVVTTLVPSSDIPHYYPTKRPLLLKL
                     IADRKTRKILGIQGVGPGEVVKRIDVLATAMSLGATIDDLPSFDLGYAPPYSTAIDIS
                     AHAANVLRNKTDGIAPFLTPMEVKEMADRGEDFLWLDVRSPEEHKQTRIEDPRVKLVP
                     LGMLRRRLQELPREKKVVTLCKAGLRAYEAQTILEGAGFKDAWVMDGGLEGWPYERFM
                     EKK"
BASE COUNT         1959 a         2088 c         2159 g         1874 t
ORIGIN      
        1 cccaatcaat tccagggcct tttcgaccgc cttgcgaatg gccgaataat acggctgggg
       61 atttgttttg acaatcgata ccttgctttt ggccataacg atctcctcgt cagctaaaaa
      121 aggatcccct cacccctcca tcctatgcca aaaggggaaa ggctggattc ttatggtttg
      181 gcgggtggcg aaaccggcat ggcgaatcca gcgcggtcaa agcatagccg agcctcaata
      241 cgagaccgtt gagcatttgc tgcagaatca acgtttaaaa cctccgattt cctgggatcg
      301 gcgaattccc cgatttgaaa tttctgggcc ttataggaaa ggttctccaa agctcaaatg
      361 tgccgctcaa atcccttttg aagggcggct gcccggatga ttatttaatt ccctcaagga
      421 aatgaatttt ggggagtgca ttttcgttga gacatcttag ccggtatctt cgtcccttgg
      481 ggccacatgg aggatagggt tgaatttcat gttggatcga agaaaattgg ctagatacat
      541 ttagggggac cataccacag agctctgggg gtgtcaattt aaaagggtat ctaagtcttc
      601 gaaaaggcag gggaatgacc ctcgattcaa aaaaagaggc gatgtaaaca aatctcatcg
      661 cctcttcccg attcggaact cccgttattc tggccctggg tctaacccta aacttccttt
      721 acggtgatgg cgccggtggg acagacttcg acgcaactct cgcagcccag gcattcatcc
      781 atgttcacgg gttcggattt cccgtccttc atctcaaaca cgctgaccgg gcaggtatcc
      841 acgcactcgc ccttaccgtc gcatttgtcc ttgtccacgg ttacctgata catggttcta
      901 atctcacctc cccttatgga ttcaaagctc ttttcagctc gggtatgcta aaataagtct
      961 agaggatttc gaggctattt ttaatgatcc cgatcatagg ttgacctttc ggtgatgagc
     1021 ctccattgaa aaattaatac cgaatatctg cttccttgtc aacgggaatc cagtcgaatc
     1081 tcatttatga tgactcggcg ggaagaagaa tatatcgggg aaaacgctta tctgcccgag
     1141 catgtcatcc ctttgatggt ggggatttcg caggccgagc cttttttcca ggaggatcat
     1201 ctcttcttcc taaaagacga ctggatgatc tttatcggat atcccctggg gcgggagttt
     1261 caagaagagg ctttctcccg ggttctggaa aaaatgcagg agaggtttca acccgccacc
     1321 acttggttca ttgctccggg cataccggaa agtttgctcc ctcaaatccg ggggaaggag
     1381 gtggatgaat attacaggct agacctgcgg caatccggga tgaagaggag cttgcagagg
     1441 gaagtggaaa aagcagccct tgttctgcgg gtggaagaga accgcgattg gtctcccgaa
     1501 cacgagtccc ttacgcggga attcctgaaa aacgaagact tgtctccccg ggtcagggaa
     1561 ctctacctgc ggatgcccca ggttctggcc cactccgaaa cctccctggc cctgagcgcc
     1621 cgcgattcga agggaaatct tgccgccttc ttcgtcatcg aacttggggc tcgtagattc
     1681 gctacctatg tggtaggatg ctattcgcag agccactatg tatcccacgc ctccgacctt
     1741 ttatttcaag aaatgattcg catcgcccgg gaacgaaaca aggaatacat tcacctgggg
     1801 ctgggagtca atgagggaat ccggaggttt aaaaaaaaat gggggggagt cgccagcctt
     1861 ccctactttg ccggcgaaat cgccgcaaga gagaaggttg ccctatcctg gcttcgcgcc
     1921 ctggcgtcaa aaatatgaac ctcaacgatc gaatgaaata cttcatcttc ccgggggaaa
     1981 agcagctcaa aacggtctgg gaggcggaaa aaaatggtaa gaagaactac attgtaggta
     2041 cggcccattt tttcccctat cacttccgga tttccctcgg caaaattctc cggcgatcga
     2101 agcgggcctt gtttgaaggt ccgttggacc ctcagagcat ggagaaagtg gtcactcatg
     2161 gttcgcaagg caacgggtcc atggagattt accaagccct tgatttccag gccctcaagg
     2221 aaattcgggg taaggccgct gacctgtttg gggactccca tccccttctg caattccttc
     2281 ctggaggtca aaagcccctg gaccccttgc aggctcattt cttaaagctc cgtccctggc
     2341 tggccttctt caacatttgg actttctacc tgaagtccaa gggatggacc ggctcagtgg
     2401 acctggaagc cttcgatctc gccaaaaaat tgggaaggga gattcaattc ctggagacca
     2461 tcgaagagca gattcaggtc ctcgaacaaa tcccggtgga ccggatgatc cgttttttga
     2521 aaaaagtcca tcattgggac gaatattccg aatcctacgt gcgcatgttc ctccagggcg
     2581 cgctggcaga ttggatgagt gggaccagcg attttccctc tcgctgttct ccggtcatcg
     2641 gtgaacggga ccgggtcttt tattgccgga tgaagccctt catcgaaaaa ggagagtcgg
     2701 ccgtgtttct cggagctccc caccttcatg gagtcaatca gatgcttgaa gccgatggtt
     2761 accgcgtgga acagacccgg gaaatcgagg aatgatcact ccccaagaag aagattttct
     2821 tacccttcac gcgtatgtgc cggaacacct accggggtac ggtagggtct tttccggcgg
     2881 gggagagcct tttctcgtag gaagctatct gggttacctt cgagaagaaa cgctcatatg
     2941 ggtggggtat cccctggaag gggcctttct ggaggaagag atgaagtccg ctctggaaac
     3001 ggccctaaag aaataccggg tctcccgggt ggcgatcacg gcttcctcct tatctgggat
     3061 gggcggggga aaaaaaacgg tcgacttcta ctaccggttg gagttgactt ccctgcgggt
     3121 agcgtcgaaa acaaaaaata tggttcaccg ggcagggcgg gagctgacca tcgacaaagg
     3181 cccggagttc gggggagatc accaggcgct catcgatctt tttcttggat cgcgggaact
     3241 ggaagaagga acgcgcatca ttttcagcag gatcgccgac tatctttctt ccgtccccgc
     3301 ggctcgggtc ttcgcggccc gggattccag gggcacactg gcgggcttcg atgtggccga
     3361 tttcggggcc ggagagtatg ctttctacct gttcaacttt cgttcccagg aggccgctgt
     3421 cccggggacc tcggacttgc tccttcatgc tctgatccgg gaggcgcagg aacggggaaa
     3481 aaggtatttg aacctcggat tgggaatcag tgaaggagtg gccttcttca agaagaaatg
     3541 gggaggccgg cctttccttc ctcacaaatc cctgatctac cggccctccc ctccctccat
     3601 cttcgatttc ctcatgaaag gattgaccca ggtatgttaa aagggctctg gaaaaaaata
     3661 tttccggctt ccccccgcaa gaaattcgcg gcctggcagg tggaactgac ttcccggtgc
     3721 cccctgcagt gcaagatgtg tagccggacg gcctacaaag attttttttc gagggatatg
     3781 agcctcgatg acttccaaaa gctgctcccc tattttcccg aggtagaggc cgtggttctt
     3841 gagggatggg gagaatccct gctgcataaa aacctcatcg agtgcattgg atgggtcaaa
     3901 gagaaagggc cccgcgcggg gtttgtaact agcgggaagg ggttgaatga atccttgatt
     3961 tgcgaactcc taaaggtggg agttgatttc atgggtttct ccctttccgg ggccaccccc
     4021 gagacccaca atgcaatccg ggtgaattct gacctggatg accttaaaaa aaacatcctg
     4081 acccttcaag agatgaaagc tcgccagaac ctggcgaatc cccatctgca cattgtttac
     4141 ctcatgctta aggaaaacat ctgtgaggtc cctgtgctta tccaattagc ccgggacttg
     4201 gggattaaac aggtaatcct gatccacctg atccaggtgt ccaattcatg gcaggaggag
     4261 caaagggttt ttggcgagaa tgggtctgag cagttcgaga atatcttgca agaagcacag
     4321 gcaaaagccc gggaatggaa aatcgaactc cagtgtcctt ctctttcttc cggcgaagtg
     4381 cccatctgcg ccgaaaagcc ccttcgcaat ctttatatct ccgtcgatgg agaagtatcc
     4441 ccttgcgtat acctgaatcc gccggtcccc acccctttca agagaatatt ccaaaaaaaa
     4501 gagttcccga tcgaaaaggt gagtttcgga aacatttttc agaaaccctt cccggagatt
     4561 tggcaaagcc cgcagtatga agaattcagg ggctgcttta cccgccgcca agaagagatg
     4621 caaaaaatga tcggggacct atgggacccg ggaaagcgaa aggaccttga gtcttattcc
     4681 ctccctcccc cccccgttcc ctgccggacc tgctacaaga tagagggctt ttaaggatct
     4741 caaatcgagg ggacctttcg gcattgaaga ataccgaaag aagttctata tcccgatttg
     4801 aaaaagggaa acaaaattgt cagccgcccg ggctggggtg tggatgggta tctcccctgg
     4861 ggcggcagaa ttcttcttta aagactggtc aacaaaacag cctcttcgac cttggcctgc
     4921 agccgcgtga gtgcctcctc ggaaaaggcc agccaggtta cctctgccct agaacccaga
     4981 attaaggacg gctcggccat cagacctatt tccattttgt gacaggccag gttggcaagc
     5041 cggacgatca ctaggagttc atcattcggg tcgatttctt ctctatggtg gtttcgggcg
     5101 acagaacagt aggcctcggg aaggtgccat ttattcagaa gagaatatcc attctccgta
     5161 tgcaagacgt tcagaacctg cctgatagcc tcttcatcca agggcaaccc tgagccgcct
     5221 tttaatttga tcttttcaat caccgtcata agaaaggaca ttcctacatc gtgaagtaat
     5281 ccagcgacaa aagcctcctg ctgcaaagaa cgaaaccggc aatttcgggc tatccattga
     5341 gctcccacag ctgatgccac ggaatgctcc cataatttgt gcatcacttt ggatacccag
     5401 gggtcctgag cggagaaatt ctcccgctgg gcgatcagga ggaggatatt catgacttca
     5461 ttggttccca ggcgcataat tgcttcccgc actgtggcca ctttctgcca gccccgatag
     5521 aaggcggaat tggccatccg cagcacccgg attgtgagag attgatcata attgatcagc
     5581 ttttccacct cgcgtatatc gagattcttt tcgcccatga accgctgaac cttctggtag
     5641 gtcgggcccg gaaggggaag ttcgaacttt tcatcccgca tgaacttgcc gataacttcg
     5701 atccagttct ttatctccat tttacctctc ctcaaaagct ccgtaacttg gccaggaaat
     5761 gaattgcggg agattcttca ggccatttca cgaaccgatc cctgaataag atggctaagg
     5821 tcaacttcaa ggcctaatga tcagtaaatc gggcaagatt gatggaaaga aatttacatc
     5881 tttattgaaa agctgtcaaa ctcaatttca ggaagaaaag ttttggcggg ttgtctatta
     5941 aaagggcttc cgatatccag gcattccctg agaaatcaag gccgcgcctt cgatggatct
     6001 tccatgaacg ggcgggagat tttcagcctg tccatcattc cccttctctc cttcggcgcc
     6061 tgcattataa taaataagga taggaaggtt taacatgaag gtttcacggg gccagaaact
     6121 taccgggaga aggtgaatcg gagaaaacgt cttctccccc ggcgagcgcg cgcatgataa
     6181 atccaagccg agcaaacagg aggaagggat gcaatcggag agaatgaaag ttgtaatcgt
     6241 tggaggagtg gccgtcgggc ccaaaacggc tgcccgtctg cggcgtctaa acccggaggc
     6301 agaaattaca atcctagaga aagggaaagt gttgtcctat gccggctgtg gcatgccata
     6361 ttatgtgagc ggggatgtgc ccgattccaa gaatctgatg gacaccccgg ccggagtcat
     6421 ccgggattcc cttttcttct tgaatgtgaa agggatcact gtcctggatc gcaccctggc
     6481 ggagaagatc gatcgaaaag caaaaaccgt atccgtggtt cacctggaaa ccggggaaca
     6541 caaggccctg ccgtacgaca agctggttct ggccaccggg ggctcgccca tcgagcttcc
     6601 gattccgggc aaagagttga atcacgtttt ccgcatgtgg cagccggaag acgccctagc
     6661 catgcgctcg ttcatccttg aaaaaaagcc caggaggggg gtgatcatcg gcggaggctt
     6721 gatcggaatc gagatgaccg aagccctcgt taaacaaggt cttaaggttt cggtcgtgga
     6781 gatgcttcct tttatcctgc ccggcctcct ggacgtggag gtcgccacct acctgaaccg
     6841 gtatcttcgg atgaaaggag tggacgtccg ctgcagcaca cgagtgaccc ggatcgaagg
     6901 cgatgaccag ggaaacgtgc agaaagtcat caccgaaaag ggggatatcc ctgcagactt
     6961 ggtgctgatt tccgtcggcg ttcggccgaa ctccaagctg gctaaagaag ccggtctgga
     7021 ggtcggggaa atgggggata tccgggccaa cgaatacctc cagaccaacg acccagacat
     7081 atatgccggg ggcgattgtg tgcagaatat tcatctttta accgggaaag ccgtcttcgc
     7141 tcccatgggt tctacggcca acaagcacgg acggctgatc gccaataaca tcatggggta
     7201 cggggagagg tttaaaggga tcctcagcac ggcggtggtc aaagtcttcg actacaacgt
     7261 gggtaagtca ggattgaccg agtcccaggc tcgggaagcc gggtttgagg tggtgaccac
     7321 tctggtccca tcctccgata tcccccatta ctacccgacc aagagacctc tccttctcaa
     7381 actgatcgcc gaccgaaaaa cgaggaaaat tctcggaatt cagggagtgg ggcctggaga
     7441 ggtagtgaag cggatcgatg tgttagccac ggccatgtct ctcggagcga ccatcgacga
     7501 tcttccatct ttcgatctgg gctatgcacc gccctattcc acggccatcg acatttccgc
     7561 ccatgcggcc aacgtgctga ggaacaagac cgacgggata gctccattcc tcactcccat
     7621 ggaagtcaag gaaatggctg accggggaga ggatttcctc tggctggacg tgcgctctcc
     7681 ggaagaacac aaacaaaccc ggatcgagga cccccgggtg aagctcgtac ccctgggaat
     7741 gctgcggcgc cgcctccagg aactcccgcg agaaaaaaag gtggtcaccc tttgcaaggc
     7801 cgggctgcga gcctacgaag cccagacgat cctcgagggt gccgggttta aggatgcgtg
     7861 ggtcatggat ggcggcctgg agggatggcc gtacgaaaga ttcatggaga agaaataacc
     7921 ttgcacgtgg aagggatata gcgaggcttt gaaaaagctt atccctgagt ctttctcaga
     7981 cccgcttttt ctcggttatt tcatcgatca tctttctctg tttctcaggc gccggctgaa
     8041 gaggagatag gggtttcccg gagcctatcc ccaaggttga
//
LOCUS       DASPRR010000087        24436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2280760, whole genome shotgun sequence.
ACCESSION   DASPRR010000087 DASPRR010000000
VERSION     DASPRR010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 24436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24436)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24436
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280760"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..1598
                     /locus_tag="VEL68_03015"
     CDS             504..1598
                     /locus_tag="VEL68_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34970.1"
                     /translation="MPQLLNSVQSEPEILPLKPVEAILPVPSPPKPPDKRIARKKSGG
                     LFSVLDYFFSKEKKDSQDFYAEVLELAQKNPENAAFQVKLAEVYQRKGKEEKAIAKYL
                     QAAEVFCRGKFFPQAMVIYKQIISINPHLIHANQKMGEIYRKMNYFSEAIAQYKIVAK
                     HYEQWGKKERVPEVLKLIQEMEREKNVREKKATPYERQKPLESQEDRRALSLPQPDPS
                     SPTPQKGGLPGDKQGQGFDLVAELAIKDPGDSMDPKEITTQKPFGFEEIFKELQETVI
                     PTEVYPDFNFQMGKACKEMGFNDGAIEQFQIALEKGQKPAEAAKLLSKCYREKGWFHE
                     AQKCYEKAMEIESYNRSKNSSTSELGMVCS"
     gene            2214..2636
                     /locus_tag="VEL68_03020"
     CDS             2214..2636
                     /locus_tag="VEL68_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXZ34971.1"
                     /translation="MVPIERIRNVEIFQGLSDEDLKIVSPYCTAEKMLSGTALCEEGG
                     RADKLFILEEGTVSIRFKKGVSFEIQGPGKILGWSFLVPPNRYTASAVAIAPSQLLVI
                     TSPDFYDLVHRDSRLGLKIMDNLARVVSNRLKAFVEIH"
     gene            2752..3840
                     /locus_tag="VEL68_03025"
     CDS             2752..3840
                     /locus_tag="VEL68_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ34972.1"
                     /translation="MGIADKIGSLLKKGNPKSGDPMDRYLSLVKKEPGNATAHLRLAE
                     LYQKKGEKKKAVAEYLMAAEIFLKNQFYARAMAIYKQVPKQDPSLDHVYLKIADIYRK
                     MGFTGDAFAQYRVLAQHYNSQGLKEKALEVLNLMADMDPQRADPNEKVQVHQASLKSP
                     EEPAVARASQPQPLPAEPKREQGKPEFFDLGAILESAGSSPMNDFKEISQVEKVYGFE
                     EIFKELKENSGPSAVDPNFNYNLGVASRELGFLEDAIEQFQVALEKNQNAFEAATLMS
                     LCYKEKGQWSEAAQALKKALQIQGIPKEKMIEVKYELGLICKQEGKTDEALGLMREIL
                     AVDQKFRDAKNEFTQLMEQGSPQGKSLA"
     gene            3862..4443
                     /locus_tag="VEL68_03030"
                     /pseudo
     CDS             3862..4443
                     /locus_tag="VEL68_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
     gene            5219..7024
                     /locus_tag="VEL68_03035"
     CDS             5219..7024
                     /locus_tag="VEL68_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ34973.1"
                     /translation="MEKAWFAHQEFLDQGDWERSRAELEKVYQWKLDQGVRNHYTYAL
                     ALIRESQKAAEKEIGVVPSELLNYAERMAPDFSEVHRARAGWLGSQTFSSFGNATQSA
                     WSWFQGIFLSFFNQEEALPRLVNLTFWVLLSFFLTFAGFSISRFFRHYFFFTHHMRHL
                     IRLRISSIPMIFFSFLFLFSPLFLGLGWMWLFVLWILAFWPYGGRADRVTTLVLLGLL
                     LLLPAGVRFHASLFHSLTGNGIPEILRANTGAWNDELYQKLLQINKKNPQDSDVLITV
                     GLVEKRMGKYAEAEQRFSQGAQLAPTSAVFNNLGNVFLVTNRLDLAMEAYQQAARLDP
                     SRAETYYNLGQAYLLKLRMKEAEAQFQRAKGLHPPKISYHTSIASKNPNRVVIDQTLD
                     PVQVWKKVLTPSPESDRIAQSLWIFLWKGIPLDRGEMAMAALLGLLALVHLGSRKISL
                     IRHCERCGGLICPLCTPFKGNGTQCVPCQNAFAANTTADPDVVRKKRAEVARYQTSVH
                     VLPQRLSWVLPGVGHLMRGRSVEGIVYLFFLSLLGTRLIWWEGWIPHPLDLNASLSIP
                     WLAVTLLLLLLLYGFVQYRMKCIRVQEVKSNFRRT"
     gene            7044..8075
                     /locus_tag="VEL68_03040"
     CDS             7044..8075
                     /locus_tag="VEL68_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXZ34974.1"
                     /translation="MTGSLKEFGLTDLFQVLGQQQKTGVLNLEGDEKKVQVFFEKGMI
                     VRVEFPEETGEDIAFGRRLIRGGLISAENWGRAFQQHEENLIGLEKVLVSSGLVTKED
                     LSAAIRLLTVDTLYGLFKWKKGNFRFETRPVSYDPEFVEPMNTEFLLLDVLRMVDEWP
                     MVAKRLSSFDMALQKVNPLATLEVLVGTPWEKNRTFQMEVIYDLVDGQRSVKEIIDLS
                     FVEEFETCKNLILMMDAGLIEPSVVKVKKERESGKGGHLADHLADVGAYIVVGVLTFL
                     LFFQFVGTRLPHFPFGDEELQAWQRVHDSLNKVQEVKWKNAQEVFFLEEGRYSQNPTE
                     LVSKRLLPP"
     gene            complement(8111..9424)
                     /locus_tag="VEL68_03045"
     CDS             complement(8111..9424)
                     /locus_tag="VEL68_03045"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HXZ34975.1"
                     /translation="MHPELIYGLDQRPSWPQSLVYGFQWTIIFLPTLMILSAVSSAYL
                     GFNDQDKLLFFQRVLLTSGGIMVFQTLWGHRYPLLDGPSSALLLSLLILAPGGMPAIQ
                     GGMISGGILLCLLSGLGLMKRLQVLFTDQVVSVTLILIAITLLPYLAPLVIGRHPGRP
                     QGDPVILGISLATVLAISLFSQKLSGFFRAVSLLLGIGFGTFLMGVLDRVPFPPPLEG
                     PWLAFPEPLFPGLPCFDLSAILPFLVAYVAVIINGVGSIYSIGEVVGKGKMEERVSRG
                     IGFTGLGGLLAGVLGSIGTVSFGISPGVVLMTRVGSQYPVTFCGIFLCLLAFFHEVLV
                     FLMSIPPSVVGAALLAAMASQVGAGISILTRLGKGMDSRDYLVVGLPILLGGVITLLP
                     ETFFQTLSPSLQAFLKNGLAVGIVAVLFLEHVFLRMGKPNRKGNE"
     gene            complement(9523..10431)
                     /locus_tag="VEL68_03050"
     CDS             complement(9523..10431)
                     /locus_tag="VEL68_03050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXZ34976.1"
                     /translation="MKKNIFCTTALSFLLVLFLAGCAEVVQMGTAVGEGMGKISKEDR
                     EAIDRAAKQTAKAARPMTEQEEYYVGRAVAATILGRYRLYSNERLTAYINSIGQSLAL
                     ASDRSYTFGGYHFAILDTEEANALACPGGIIFITRGMLKEAQNEDQLAAILAHEVAHV
                     NHKDGLASIQKSRWMEALSILGSETARKIGGAEMTKLVSIFEGSVDDVAKTLLVNGYG
                     REQEMKADLGALSFLNRLGYNPYGLTDSLEKMAREQSGGSKQGIFATHPGMNQRLASS
                     RSTLAKNQWPRKDDPARDRRFREFVG"
     gene            complement(10428..10904)
                     /locus_tag="VEL68_03055"
     CDS             complement(10428..10904)
                     /locus_tag="VEL68_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXZ34977.1"
                     /translation="MKTKTWVILLALCLISSVAAAATVKVITQEAMIRTDKRFFAPAL
                     VRAPFGAALEELGREGDWFRVNYQGKEGWIHKSAVQEQKFQLSSLAGGQADEASRDEV
                     ALAGKGFNPEVEKAFRDKNPKMRYDLVNQVQAYRVEEQKLQAFIRAGNLREPGGAQ"
     gene            complement(11046..13196)
                     /locus_tag="VEL68_03060"
     CDS             complement(11046..13196)
                     /locus_tag="VEL68_03060"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXZ34978.1"
                     /translation="MSDQLFSSPKRKVLSGAIIGFLAWVSVLLLYSGNLLESYELKTY
                     DQLCRLQAPRSPAPGEVVLVVLDQSSLEAAQKEGINWPWPRQMYAPILQFCAAAGARA
                     VAFDILYTEPSSYGVEDDQVLAEALKVNGRAILPIFLSNQTRPHPSYEMDLLDRISIP
                     LKNDSGQEPTSYLSSLPPIQALAESARALGNVAIFPDSDGIYRRIPVIFSYQQRWIPS
                     LGLAAFRGVADQDQAVLEKNSFKIKNTSFPLDSRQNFLLYYYSQENDFRRFSAFNLIQ
                     SSLALQEGRKPAYPLESFRDKIVFVGFTAPGLFDLKPTPVSSVTPGMVVHATFVANLL
                     RGDFRVRVSSSFILPLALGLALAMAIIVLLVSSLWRLALMVLACAGALAFFILFSFHR
                     NFWFDAVLLASCPGLSFAISTAFSYATEGRQKRQIKQAFSHYMSDLLIQDLLKHPEKL
                     RLGGERRVLSVFFSDLAGFTSLSEKLSPEGVVTLLNRYLTAMTDIILASGGIIDKYEG
                     DAIMAFWGAPLPQEDHAARACLAALDNQARLVDLRQEFAQTGLPPVFARIGINTGEMI
                     IGNMGSSQRFDFTVIGDSVNLASRLEGAGKEYGVSILISEDTYRQAKDAVEVRELDLL
                     QVKGKERPVRIYELLAKKGELPSPKARVREVFAEGLALYRKQAWDEAVPRFQQVLELS
                     SEDGPARTFIRRCQVYRENPPGPAWDGVYRLTTK"
     gene            13479..14873
                     /locus_tag="VEL68_03065"
     CDS             13479..14873
                     /locus_tag="VEL68_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ34979.1"
                     /translation="MRSIQEVLDAKSVAVIGASRDPEKPGAQLLRVLKKVGFRGQVAG
                     VNPQGGEVFETPLYRTLEEVPFPVDLAVLHIPPRLIPAVLQDCVRKRVKGVVISAEGF
                     AETGAEGAKYQEEVKSILQSSGMRGFGPNTLGLVNTATGLTTSYYAGPRMLRPGPIGF
                     AAQSGIFVGALLRYLSSFEGFQLSKGIGLGNKVDVNESDALSYLMDDEQTQIIGMYLE
                     DVRDGRRFLEIARQAVAKKPVLLIKGGRTREGARATASHTASMAVEDSVFEGAVRQAG
                     ALRLTGIDDFVRALKGFLNMPLPKGGRLAFVTYSGAQAIMSIDASVDHGLQVARLDES
                     TRERIGRVIATASKTQNPVDIFPDWLANGFEKTTTEILKAFLEDEGVDGIICISFANA
                     GAEIYQPMVDMLKGERTKPVFFSLLGAKKDIDVCQNLLEENRIPCFDFPEMAVRVFAH
                     MWKYAQTKQKAHRA"
     gene            15067..15453
                     /locus_tag="VEL68_03070"
                     /pseudo
     CDS             15067..15453
                     /locus_tag="VEL68_03070"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
     gene            complement(15491..15772)
                     /locus_tag="VEL68_03075"
     CDS             complement(15491..15772)
                     /locus_tag="VEL68_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXZ34980.1"
                     /translation="MGDKPQTLRKEIAKALMDGPMDLREISQVFRIREKDVLDHLQHI
                     SRSAQLKMEPAVCLKCGFSFKKRDRLNTPSRCPLCKSESISPPRYRVLV"
     gene            complement(15820..17070)
                     /locus_tag="VEL68_03080"
     CDS             complement(15820..17070)
                     /locus_tag="VEL68_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HXZ34981.1"
                     /translation="MAQELRDPKEKDKNRLRASQKMKELMTRYYMGAKMAEGTDKKIA
                     WITSGGPVEPLIAADIIPVYPENHGAMLGASRMSVELCEVAEGLGYSRDLCSYARGDI
                     GSALTKKSPIPGGLPKPDFLLACNNICGTVMKWYQSLSRIFNVPLFLLDTPFIHHHVV
                     PESLIYVRQQFEELISFLESITRKKFDRDRLTEVLRNSGESALLWKEVLDLCTHRPSP
                     MSCFDAFINMAPIVTLRGTKEVTGFYRDMKAELQERIALGISAVPQERFRLLWDNIPI
                     WYKMRDLSELFSSFGACLVADTYTSAWVFEGFETLDPLDGLSQAYTKIYLNISIDLMI
                     EKVLNLTKRFSVDGMVIHSNRSCKPYSLGQYDIQRIVLEKTGTPSLVLEADMTDSRVY
                     SDAQVKTRVEAFMETLENRKAARA"
     gene            complement(17155..18285)
                     /locus_tag="VEL68_03085"
     CDS             complement(17155..18285)
                     /locus_tag="VEL68_03085"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HXZ34982.1"
                     /translation="MEKQNAFGALKGYAADPSAYLKNWKDKTGKKILGYFCTNTPEEM
                     IQAAGFLPVRILSSRDTISLASRHLQSYSCSLVQSSLESGLRGELSYLDGTVFPHTCD
                     SIQRLSDIWAENLCFPFHWDLVLPVKLHTESARNYLIQELRRFRGGLAEYIGHPISDA
                     DLRGTFHLFNENRSYLREVSRYQSSYPGWISAADSHALIKTANFMAKDEHNARMKELL
                     SQLGNGQAPSPHSVRLFLVGSVCDQSSIYGLIDSCGAALVGDDLCTGWRYFAEDLPNE
                     GDPIEALADRLIRKVPCPCKFNPEIDRADRLLERVNAAETQGVVFLLLKFCDPHAFDY
                     PYLKERLSKEKIPSLLLEIEPGGLPLGAMETRLRAFVETLSR"
     gene            18700..19860
                     /locus_tag="VEL68_03090"
     CDS             18700..19860
                     /locus_tag="VEL68_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXZ34983.1"
                     /translation="MRTGYFFIPTLLFIANFYLYNWIVRFTQPSVWVRRAILLGLFLL
                     LALFPVSRLWAAYDFNSLNRLITFLASSWIGISFLLVLCAGGTDLIRFILRKTGLSPK
                     VSFFQGLYYRRVAVAASVAGCLALGGYGFWEAGHVGVTRIEIPLRHLPPDLDGLSVVL
                     LSDVHYGMLHENGRLSDLVRRVNELQPDLVVFTGDLVDADVSHMEEMAGPLSGLKATY
                     GLFAVMGNHEFFAGANRVEAIMKQAGIQVLRNEVRVLPGGLQVLGIDDPAVVRRGGPL
                     PNFEGLIRGLDAHKPSILLYHQPIRFEEVAAGKVGLQLSGHIHGPQLLPMVPLARLFY
                     PRMQGLFRLEDSYLYVSRGVGTGGPPMRLGSPPEIVHIRLRSPKSSILNHDS"
     gene            complement(19843..22521)
                     /gene="gcvT"
                     /locus_tag="VEL68_03095"
     CDS             complement(19843..22521)
                     /gene="gcvT"
                     /locus_tag="VEL68_03095"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXZ34984.1"
                     /translation="MTSEPKKTPLNAWHRSHGAHMVEFAGWEMPLNYPPGIVEEHLTT
                     RRFCGLFDISHMGRFRISGEGAVPFLQYALTNNALALDPGQAQYTIISDENGTAVDDA
                     YLYRLDEEEYRLVVNAANTQKDWDWLQNQRKKFSRVEMENHTARIAMFSLQGPLSKAL
                     LETILQGSGSRLPDPGKNNERRVWIEGTPVDVGRTGYTGEPLGFEIFFPAERAVVIWE
                     KILRQGSERGVIPIGLGARDTLRLEAGLPLYGHEYGKDQEGRDIPILALTPAKIAVSF
                     SESKGDYLGRQSLWKQLQEIQAREYGWNDLIPSTPLVPRRIWPVAVLSEGVVRQGFPV
                     FAGDRPVGYVTSGTMVPYWKFEGQGISSRVSREKGRRSIALVYLDADLKEEQEVKISA
                     RGKLLTGRLVRRHLGAEAPPYARPVLAEEILIKRPGPREPLHFSTEILVRKARENTLW
                     RQRQTINLIPSEQTPSPLVKLLSISDPCSRYAEHRNYEFLGEADVFYYQGTDFIEWVE
                     VKLQEEMARFLGCTMVETRIISGQMANAAVYSSFVDYLNRVYRKTNPRRFRKVMNHHL
                     GRGGHLSSQPMGALRDFISVAPDLDRMAAVNFPVLEENPYQIDLKQTEELIHRHKPEL
                     LILGKSMVLYREPLAELRRMVARLKEKTWILYDMAHVLGLIGPHFQDPFREGADIVTG
                     STHKTFFGTQRGIIASNLDETSEYYDLWKAITRRTFPGSLSNHHLGTLLGLLLAAYEM
                     NAFAGDYQKQIIANAKAFAKALKDQGLQVEGDPGVGFTETHQVILRVGYTLGPETARR
                     LEENNVIVNYQALPDDEGFSASSGLRTGVQEMTRFGMKEKDFAELAGLMADAILKNRL
                     VKDEISRLRSPFLQMQYCLPEEQARPMIQDLLGIMI"
     gene            22896..23219
                     /locus_tag="VEL68_03100"
     CDS             22896..23219
                     /locus_tag="VEL68_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HXZ34985.1"
                     /translation="MTESTTATGTVTREKLMEDLKAVVNDAEELLKATANQTGEKIAS
                     ARAKAEESLKTAKARIAEEGKAVMEKAKSAAKSTDDFVRDQPWKAVGIGALAGFVVGL
                     LISRR"
     gene            23286..23636
                     /locus_tag="VEL68_03105"
     CDS             23286..23636
                     /locus_tag="VEL68_03105"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXZ34986.1"
                     /translation="MKALAAALLAIAYTRLELFSVELEEEWVRISSILTWSLVALFCA
                     GIGIVFTTLFLVYALWGTHPLVALGIPAVLFLLFAALAWRIVAAKVRTKPRMFSASLA
                     ELAKDREEFLKSDS"
BASE COUNT         5586 a         6609 c         6987 g         5254 t
ORIGIN      
        1 gccgcgccag ggccttcgaa tggtccgggg actttatggc cgaagaaccc ctctgcattt
       61 accaacccca aggttcctga aggtttccat ccctggcaac cccggtaatt actcaaccca
      121 gcgcagccct ttccgtcctg aaattttccc tccgagaata tcaccgctgg actccattga
      181 agcaagagcc aaagcccttt tcagaccatc cttttttgga caaagcccat tggggatttg
      241 tacaattatg agcggaattg ggggcgcttc gatccacctg gtttagggcc ataaggaaga
      301 ataaccctgc aaaatgattt gcctcaattc gaaaatcatc tgtgttataa atgggttcgg
      361 cgatgaatcc atcccaaaag cagtaggatt cgggtttatc gatctgatga tttgcctgag
      421 ggcacaaaaa ttgcgggcat cgaaaatgcc gctggaggaa tctcttcttc cttcagaatc
      481 taaggcgagc aaggatatcg aaaatgcctc agctattgaa ttctgtccag agcgagcccg
      541 agattctccc gctgaagccg gtagaagcga tccttccagt cccgtccccc ccaaaacctc
      601 cggataagag aatcgcgcgg aaaaagtcag gcggtctttt cagcgtgctg gattattttt
      661 ttagcaagga gaaaaaagat tcgcaggact tttacgcaga ggttctggaa ctggctcaaa
      721 aaaatccaga gaatgccgcc ttccaggtga aactggccga agtctaccag aggaagggga
      781 aagaagagaa agcgatcgcc aagtatctcc aggcagcgga ggtcttctgc cggggaaaat
      841 tcttccctca agccatggtc atctacaaac agatcatctc cataaacccc catctgatcc
      901 acgcgaacca aaagatgggg gagatctacc gtaagatgaa ctatttctcg gaggccattg
      961 cccagtacaa gatcgtggcc aaacactatg agcagtgggg gaaaaaagag cgagttcccg
     1021 aagtcctaaa gctgattcaa gagatggaga gggaaaaaaa cgtccgcgaa aagaaggcca
     1081 caccgtacga aaggcaaaaa ccacttgaat cccaagagga ccgtagggct ctctctcttc
     1141 cccagccgga tccttcatct cccaccccac aaaaaggagg tctcccgggc gacaagcaag
     1201 gtcagggatt cgacctcgtt gccgagttgg ccatcaaaga ccccggggat tccatggatc
     1261 ccaaggagat caccacgcaa aagcctttcg gctttgagga aatcttcaaa gaactgcagg
     1321 aaacggtcat ccccactgaa gtatacccgg actttaactt ccaaatggga aaagcctgca
     1381 aggaaatggg gttcaatgac ggagcgatcg aacagtttca gatcgccctc gaaaagggcc
     1441 agaagccagc cgaggcggct aagctgctaa gcaagtgtta ccgggagaag ggatggttcc
     1501 acgaagctca gaagtgctat gaaaaggcca tggagataga aagctataac cggagtaaga
     1561 attcaagtac cagtgaattg ggcatggttt gcagctgagt tgtcttaggg cctggattcc
     1621 gccaaggccc acccctcctt cctccaaggc agccggcggt tccccttccc ctttcgcctt
     1681 ctcttggtga ttccctgcct gaaaatccat ccccttattt tctatcagtg gctcgaggcc
     1741 agataacggg acgcaaaaat gatcagaccc aagggagcag ggggtaccat tgccacccct
     1801 gagtaggcag aggggcctgt tttttaccat taaccgaaac cacattcccc gggagaggac
     1861 caatagggaa ccaggaaaaa tcccatgccg ggctgatgaa taagttggaa caaataccta
     1921 ggggtactca tcggccagaa ccatacccgc aggcttgcgg ggaccccaaa gaagtggtag
     1981 gatcgcctga aagggatgtc ttccgaatat ccacctgggg caacccgcct tctccagggg
     2041 ttttgatgta ccagggggag ggggtttttt tctaaagttt tccaggaaat cgaccgaaat
     2101 caatagcaga aacccttaga aggaatggat attccgggaa ccctttcgcc tcaaagccaa
     2161 agagaatatc agactccggg tctcgaagga acccgggggg agaggagaaa aaaatggttc
     2221 ctatcgagag aatccggaat gtcgaaatct tccaggggct cagtgacgaa gaccttaaaa
     2281 tcgtctcccc gtactgtacg gcggagaaga tgctttccgg aaccgctctt tgcgaagagg
     2341 gggggcgagc cgataaactc ttcatcctgg aggaagggac agtttccata agattcaaaa
     2401 aaggggtgag ctttgagatc caggggccgg gaaaaatctt gggctggtct tttctggttc
     2461 ctccgaatcg atatacggct tccgcagtag ccatcgcccc atcccaactc ttggtcataa
     2521 cgagtccgga tttctacgat ctggtccata gagatagccg attggggcta aagattatgg
     2581 acaaccttgc gcgggtggtt tccaaccgct tgaaagcctt cgtagagatt cactgaaaat
     2641 ccatccggag cgcgggcagg ccagcgcgtc ctccggggct ggaagaagat gaaaccccct
     2701 gccgtggggc gggggaacaa agggaaccct accctcaact gagaaaggcc tatgggaatc
     2761 gcagacaaga tcggtagtct tctgaagaaa gggaacccca agtctgggga cccgatggac
     2821 cgttatctgt cattggtgaa gaaggagccg ggcaatgcca cggcccattt gagattggca
     2881 gagctttatc agaagaaggg agaaaagaag aaggccgtcg ccgaatattt aatggccgcg
     2941 gagatttttc tgaaaaatca gttttacgcc cgggccatgg ccatctacaa acaggttccc
     3001 aagcaggacc catccctgga ccacgtgtac ctgaaaatcg ccgatattta ccgtaaaatg
     3061 ggcttcaccg gggacgcctt tgcccaatac cgggtcctgg cccagcatta taatagccaa
     3121 ggtttgaagg aaaaggccct tgaagtgctg aacctgatgg ccgacatgga cccccaaagg
     3181 gccgatccaa acgagaaggt ccaagtccac caggccagcc tgaaatcacc cgaagaaccc
     3241 gcggttgcaa gggcctctca accccagccc ctcccggccg agcctaagag agaacagggg
     3301 aaaccggaat tcttcgacct gggagccata ctggaatcag caggctcctc cccgatgaac
     3361 gatttcaaag aaatttccca agtggaaaag gtctatggat ttgaggagat cttcaaagaa
     3421 ctgaaagaga acagcggccc cagtgcagtg gaccccaatt tcaactataa cctcggggtc
     3481 gcctcccgcg aattgggatt cctggaggat gccatcgagc aattccaggt cgccctggag
     3541 aaaaaccaga acgcctttga ggcggccacc ttgatgagcc tgtgttacaa ggaaaagggg
     3601 caatggagcg aagcggccca ggccttgaag aaggcgcttc aaatccaggg gattcccaag
     3661 gagaagatga tagaggtcaa gtacgagctg gggttaatct gcaaacaaga aggcaaaaca
     3721 gacgaggccc tgggacttat gagggagatt ttggcggtag accagaaatt tcgggacgcc
     3781 aagaatgaat tcacccaact catggagcag gggagccctc aggggaaatc cctagcttaa
     3841 ggaaaaccgg aggggagagg aatggcggtc ttcaattatg ccaaaaaaga gatcgatgcc
     3901 aagatcgtct attatggccc ggcaatttcc ggaaagacga caaaccttca gttcatccat
     3961 cagcacttga aacccgatca aagggggaaa atcgtctccc tggcaaccaa cgaggaccgg
     4021 accctgttct tcgattttct acccatcgaa ctagaaagcg ttcggggttt taaaacccga
     4081 attcatctgt acacggtccc cggccaggtg tattacgggg ccactcgaag ggcggtgctc
     4141 accggtgcag acggggtggt ctttgtcgcc gattcccatg ccaaccgcat ggaggataat
     4201 ctgatcagtt tcaaagacct ggaggataat ctccggtatt atggaaaaaa gatcgaaacc
     4261 attcctttaa tcatccagta caacaagagg gacctaccca atatcctgag cgtggaagag
     4321 ctgaaccaga aattcaaccg gttcaacagt ccctcttttg aatcggtggc cactctgggg
     4381 aagggggtat ttgaaagcct gaccatggcc tgccgactgg ttctgaaggc catcgaaagc
     4441 ggagttgagt cgcgacgttc cccgggagtg gcggaaaaga cccctatggc ggtttcgaaa
     4501 ggtgtttccc cgggaaaacc gacctcttcc cccgaacccc atctgccccg tccggagcct
     4561 gccccctccg tcagaactct aggcatggaa aaagcgagtg ccgcggccaa gggtggccaa
     4621 ccaattctgg aaacccctcg agcggggtcg ttgcctaagg aaaaggaggc tcctccttca
     4681 gaggcgggga atgcggggaa atccccctct ttaaggatcg aaagaccgga ggagcagaaa
     4741 aagccggtcg gcccacctct ggtcgggaaa acagtagaaa aaaagaaacc ggacttctcc
     4801 ttcaaactgg aaaagggggc ggagataaaa cctctccctt ccgtggagaa aaaagttccc
     4861 catgggaatg agaatgtcag gattgtttcc tgcggccagg ccagggtctc ttctcccggg
     4921 agcctggaga ttccgctgct cctggaggtc gaagctgctc aaaagagctt ttctctcagc
     4981 gtcaaccttt cggtcaaact tgaatccctt gaagtaagac acgaataaga aagccatgcc
     5041 aggactcctt tccgccagtc cccctctcgg tcgcttctct ttggtgtttg ccggaggcca
     5101 aagggggatt gggctgctgg gcatcgtcct cctcttggga ggactggttt tgggggcctc
     5161 cttcggctgg gctcaggtac cctccttccc aatccagacg tcaattcaga aggacgaaat
     5221 ggaaaaagcc tggttcgcgc atcaggaatt tctggatcag ggggactggg aaagaagtcg
     5281 ggccgagctc gagaaagtgt accaatggaa gctggaccaa ggagttcgca atcattatac
     5341 ctacgctttg gccttgattc gggaaagtca gaaggccgcc gaaaaggaga taggagtcgt
     5401 tccctctgaa cttctcaact atgcggagcg aatggcgccc gacttctcgg aagtccaccg
     5461 ggctcgtgcc gggtggctgg ggtcacaaac attcagttcc ttcggaaatg ccacccaatc
     5521 agcctggagt tggttccagg gaatttttct ttctttcttc aatcaggagg aagccctccc
     5581 ccggttggtg aacctgacat tctgggtcct attgagtttc ttcctcacct ttgcgggttt
     5641 ttcaatctct cgattcttcc ggcactactt cttttttacc catcatatga ggcatttgat
     5701 ccgcctgcga atcagctcca tccccatgat attctttagt ttcctctttc ttttctcccc
     5761 cctttttctc ggcctgggct ggatgtggct gtttgtcttg tggattctgg ccttttggcc
     5821 ctatggcgga agagcggacc gggtgacgac ccttgtcctt ctgggacttc tccttctact
     5881 ccccgcaggg gtccgtttcc atgcctcttt attccattcc ctcacaggca acggcatccc
     5941 cgaaatcctc cgggccaaca cgggagcgtg gaatgacgag ctttaccaga agctgctcca
     6001 gataaacaaa aagaaccccc aggattccga tgtcctgata accgtagggt tggtggaaaa
     6061 acgaatgggg aagtatgctg aggcggaaca gcgattctcc cagggagctc agctggcccc
     6121 cacctctgca gtctttaata atttaggaaa tgtattcctg gtgactaacc gtctcgacct
     6181 cgccatggaa gcctatcagc aggccgcccg tctcgacccc tcccgggcgg agacttacta
     6241 caacctcgga caggcttacc tgctgaagct tcggatgaag gaggcagagg cccaattcca
     6301 gcgggccaag ggtctccatc ccccgaagat ttcctatcat accagcatag cctcgaagaa
     6361 ccccaaccgg gtggtcatag accagaccct cgatcctgtt caggtttgga aaaaggtcct
     6421 aactcccagc ccggaaagcg atcggatcgc ccagtccctc tggatttttc tctggaaagg
     6481 aatccccctg gatcgtgggg aaatggccat ggccgctctc ctcggacttc tggccctggt
     6541 ccatctgggt tcccgaaaaa tatccctaat ccgtcattgc gagcgatgtg gcggtttgat
     6601 ctgcccccta tgcacaccct ttaaaggtaa cggcacccag tgcgtaccgt gccagaacgc
     6661 gtttgcggcc aataccacgg cagaccccga cgtggtaagg aagaagaggg ctgaagtagc
     6721 ccgttaccaa actagcgtgc acgtcctgcc ccagcggctc tcctgggtcc tgccgggggt
     6781 ggggcatttg atgcgcggtc gctccgtaga aggaatcgtc tatctctttt ttttgtccct
     6841 cttagggacg cggttgatat ggtgggaagg ctggatcccc catccgttgg acctgaacgc
     6901 ctccctttcc attccctggc tggcggttac tctattgctg ttgctccttc tatatggttt
     6961 cgtccaatac cggatgaaat gcatccgcgt gcaggaggtg aaatccaatt ttcgacgcac
     7021 gtaaagacgg caaggacgtg ggtttgaccg gctccctgaa agaattcggg ctgacagacc
     7081 tgtttcaggt cttggggcag cagcagaaaa cgggggttct caaccttgag ggggacgaga
     7141 aaaaggttca ggtatttttc gagaagggaa tgatcgtgag ggtcgaattc cccgaagaga
     7201 cgggggaaga catcgccttt ggccgcaggc tgatccgggg tggcctgatt tccgctgaaa
     7261 actgggggag agccttccag cagcacgagg aaaacctgat cggtttggag aaggttctgg
     7321 tcagcagcgg cctggtcacc aaggaagacc tttccgcagc catccgcctg ctgaccgtgg
     7381 atacgcttta cggtctcttt aaatggaaga aaggaaactt ccgtttcgaa accaggccgg
     7441 tctcctacga tcctgagttc gtggaaccga tgaataccga attccttctc ctcgacgtgc
     7501 tccgcatggt tgatgaatgg cccatggtcg cgaagcggct gtccagcttc gacatggccc
     7561 tccagaaagt caaccccctg gccactcttg aagttttggt cggcaccccc tgggagaaga
     7621 accggacctt tcagatggag gtcatttacg atctggtcga cggccagcgg tcggtgaagg
     7681 aaatcatcga cctgagcttc gtggaggagt tcgagacctg caaaaacctc atcctcatga
     7741 tggatgcagg tttgatcgaa ccaagcgtgg taaaggtcaa aaaagaaagg gagagcggaa
     7801 aggggggaca cctggccgac cacctggcag atgtgggagc ctatatcgtt gttggggtcc
     7861 tgactttcct ccttttcttc cagtttgtgg gaacccgcct tcctcatttc ccctttgggg
     7921 atgaggaact gcaggcttgg cagagggtcc atgactccct aaataaggtc caggaagtga
     7981 agtggaagaa cgcccaggag gtctttttcc tggaagaagg ccgctattcc caaaacccta
     8041 cggagctggt gagtaaaagg ctccttcccc cctgaccatt ccccggtttt cctctcttgt
     8101 ctttttatcc ctattcattc cctttcctat tgggttttcc catcctcagg aagacgtgtt
     8161 ccaaaaaaag aacggccacg atgcccacgg ccaatccgtt ttttagaaaa gcctgcaggc
     8221 tgggagaaag ggtctgaaaa aaagtttcgg gcaggagagt gatgaccccg cccaaaagaa
     8281 taggaaggcc cacgaccaga taatcccggc tatccatacc cttcccgagc cgggtcagga
     8341 tcgagattcc ggctccaacc tgggaggcca tggccgcaag aagcgccgct cccaccaccg
     8401 aaggggggat ggacatgagg aagaccagga cctcatggaa aaaagccaag aggcagagaa
     8461 aaatcccaca gaaggtaacc gggtactggc tgcccactcg ggtcatgaga acaactcccg
     8521 gactgatccc aaaactgacc gtaccgatcg accccagtac cccggccaaa agccccccca
     8581 ggccggtgaa gccaattccc cgcgaaaccc gctcctccat tttccctttg ccgaccacct
     8641 cgccgatgct gtaaatgctt cccactccgt tgatgatgac cgccacgtaa gccaccagaa
     8701 agggaaggat cgcggataag tcgaagcaag ggaggccggg aaaaagaggc tcagggaagg
     8761 ccaaccaggg gccctccagg ggaggaggga aaggcactcg gtctagtact cccattagaa
     8821 aggttccgaa gcctataccc agaagcaagg aaaccgccct gaaaaaaccg gacagctttt
     8881 ggctgaagag ggaaatggcc agaacggtag cgagagaaat ccccaggatt acggggtccc
     8941 cctggggccg tcccggatgt cggccgatca ccagcggagc caggtagggc aagagggtga
     9001 tggcgatcag gattagagtc acgctgacca cctgatcggt aaaaagaact tgcagccgct
     9061 tcatgagacc cagaccgctc agaagacaaa gcaagattcc gccggagatc atccctccct
     9121 ggatggcagg cattccgccg ggggccagaa tcaagagact caaaaggagg gcggaggagg
     9181 ggccgtccag aagaggataa cgatgacccc agagggtctg gaaaaccatg atccctccgc
     9241 tggtgaggag gactcgctgg aaaaagagca acttgtcttg gtcgttaaaa cccagatagg
     9301 cggaggagac ggccgagaga atcatcaagg taggcaggaa gatgatcgtc cactgaaatc
     9361 cgtaaaccag ggattggggc caggagggtc tttggtcaag tccatagatc agttctgggt
     9421 gaatggaacc tccctttcaa cggctatctt ggtgaggaag cagatcggcc ctcattcgca
     9481 ttttgagcgc cttttccgta ttcatccgaa tccgaaacct atttacccca cgaattcccg
     9541 gaagcgccgg tctctcgccg ggtcgtcttt ccggggccac tgatttttgg ccagggttga
     9601 tcggctacta gccagacgct gattcatccc gggatgggtg gcgaagatgc cctgtttcga
     9661 tccgccgctc tgttcccggg ccattttctc cagggagtcg gtgagcccgt aagggttgta
     9721 ccccaggcgg ttcagaaaag agagggctcc gaggtccgct ttcatctcct gctcccggcc
     9781 gtatccatta accaggaggg tcttggccac atcatccacc gatccttcaa aaatcgaaac
     9841 caacttggtc atctccgccc cgccgatttt ccgggcagtt tccgagccca ggatggacag
     9901 ggcttccatc caacgagact tttgaatgga ggccaggccg tccttatgat tcacatgggc
     9961 cacttcatgg gccaggatgg cggccagttg gtcctcgttt tgggcctctt tcagcattcc
    10021 ccgggtgatg aaaatgatcc cccccgggca ggccagagcg ttcgcctctt ccgtatccag
    10081 gatggcgaag tgatagcctc caaaagtgta ggagcgatcg gaggccaggg cgagggattg
    10141 gccaatggaa ttgatataag cggttaaacg ttcattcgaa tacagccggt agcggccaag
    10201 gatggtggcg gccacagccc ggcccacata atattcctcc tgctcagtca tgggcctggc
    10261 cgctttggcg gtttgcttgg ccgcccgatc aatggcctcg cggtcttctt tggatatttt
    10321 ccccatccct tccccgacgg ccgttcccat ctgcacaacc tctgcgcaac ccgccaggaa
    10381 caagaccaga aggaaggaca gggccgtggt gcagaaaata ttttttttca ttgcgcacct
    10441 cccggttccc gaaggttacc agcccggatg aaagcctgca gtttctgttc ctccactcga
    10501 taagcctgca cctggttcac gaggtcatac cgcatcttcg gattcttgtc ccgaaacgcc
    10561 ttttccacct cgggattgaa accctttcct gccaaggcta cttcgtcccg ggaggcttcg
    10621 tccgcctggc ctccggccag ggaagagagc tggaattttt gttcctggac agcgctcttg
    10681 tgaatccagc cttcttttcc ctggtaattg acccggaacc agtctccctc cctccctaat
    10741 tcttccaggg cggccccaaa gggggcgcgg accagagcgg gagcgaaaaa acgcttgtcc
    10801 gtacggatca tcgcttcctg ggttatgacc ttcactgtcg cggctgccgc gaccgacgag
    10861 atcaggcaca gggccagaag aatgacccag gtttttgttt tcataccagc ctccctcgca
    10921 tgaagtacct agaacgcgcc agagtttcct agattatcca caattttaag agtcactccc
    10981 cggttcacgc tctcgatcaa tcccatctca agaacttcgg gtattccgaa ctttagtctc
    11041 tctagtcatt tggtcgtcaa tcgataaacc ccatcccatg ccggccccgg cgggttttcc
    11101 cggtaaacct ggcaccgtcg gataaaagtt cttgccgggc cgtcctcgga actcagttcg
    11161 agaacctgtt gaaaccgggg tacggcctcg tcccatgcct gtttccggta tagggccaac
    11221 ccttcggcaa agacctcccg cacccgggcc ttcggggatg gaagttcacc cttcttggcg
    11281 agaagctcat aaatccggac aggccgctcc tttcccttga cctggaggag gtcgagctca
    11341 cgcacttcta cagcatcttt agcttggcgg taggtgtcct cgctgatgag gatggagacc
    11401 ccgtattcct tgccggcccc ttccaggcgc gatgccaggt tcacgctgtc tccgatcacc
    11461 gtgaagtcga acctctggct ggagcccatg tttcctatga tcatctctcc cgtgttgatt
    11521 ccgatgcgcg cgaagaccgg ggggaggccc gtctgggcga actcctgtcg taaatccacc
    11581 aggcgggcct gattgtctag ggctgccagg caggcgcggg cagcgtggtc ttcctggggg
    11641 aggggggctc cccagaaagc catgatggca tcgccctcgt acttgtcgat gatccctccg
    11701 ctggccaaga tgatgtcggt catggccgtg aggtaccggt tcaaaagggt taccacccct
    11761 tccgggctga gcttttcgga taggctggtg aatccggcga gatcggagaa gaacacgctc
    11821 aaaacccttc tctccccgcc cagccgcagc ttctccgggt gcttcagcag gtcctggatc
    11881 aggaggtcgg acatgtaatg cgagaaggcc tgtttgatct gccgtttctg gcgcccttcg
    11941 gtggcgtagc tgaaagccgt gcttatagca aagctgagcc cggggcagga agccagaaga
    12001 accgcgtcga accagaagtt cctatggaag gaaaaaagga taaaaaaggc taaggccccg
    12061 gcacaggcaa ggaccatcag ggccaaccgc cagaggctgg aaacaaggag aacgatgatt
    12121 gccatggcca gggcgagccc tagggccagg ggcagaatga aggaggagga tacccgaacc
    12181 cgaaaatccc cgcggagaag gttggccacg aaggtggcgt ggacgaccat gcccggcgtc
    12241 accgagctga ccggagtcgg tttcaggtcg aacaggcccg gggcggtaaa acccacgaag
    12301 acgatcttgt cccggaagga ttccagggga taggcgggct tgcgcccctc ctgtagggcg
    12361 agggaggatt ggatcaagtt gaaagccgaa aagcggcgga aatcattttc ctggctgtag
    12421 taatagagaa ggaaattctg cctagagtcc aggggaaagg aagtgttctt gatcttgaag
    12481 gaattcttct ccaacaccgc ctggtcctgg tcggcgaccc ctcggaaggc ggccagaccc
    12541 aacgaaggaa tccatcgctg ctggtaagag aaaatcactg ggatccgccg gtaaattcca
    12601 tcggaatcgg gaaagatcgc cacatttccc agcgcccggg cgctctcggc cagggcctga
    12661 atgggcggga gggaagagag gtaggaggta ggttcttgtc ccgagtcgtt cttcaaaggg
    12721 attgaaatgc ggtcgaggag gtccatttca taagaaggat gggggcgcgt ctgattgctg
    12781 agaaagatgg gcaggatagc ccggccattg accttgaggg cctcggccaa aacctggtca
    12841 tcctccactc catacgagga gggttcggtg tagaggatgt cgaaggctac ggcccgcgcc
    12901 ccggcggcag cacagaactg caggatggga gcgtacattt gacgcggcca gggccagttg
    12961 atcccctctt tctgcgccgc ctccaggctg ctctgatcca aaaccaccag aacgacctct
    13021 cccggagccg gggaacgggg ggcctgcagt cggcacagct gatcgtaggt tttcagttcg
    13081 taggactcga gaaggtttcc tgaatagaga aggagaaccg aaacccaggc caggaacccg
    13141 atgatcgcgc cggagaggac tttccgtttt ggggaggaaa aaagctgatc ggacaactcc
    13201 caacctcaaa tgcccttgat ttttttctct accaataaat tatagcggaa tgattgcccc
    13261 ttggccagcc ccgctgatat ctccattgtt tttttccggc gccatctcca accatcgttt
    13321 cccttcattt tggtcccctt tgttatcata tacgcagagg cggttcggga atttcccccc
    13381 gtaggggcca aacaaggacg attaaaaaag ttttctctcc ccgttgaatg tgctatggta
    13441 ctcacgtttg aatggattgg tttggaaggg agctattcat gcgttcgatt caggaggtct
    13501 tggatgccaa gagtgtggcg gtcattgggg cctcgcggga cccggaaaag ccgggggcac
    13561 aactcctcag agttttgaag aaggtgggtt tccggggaca ggttgccggc gtcaaccccc
    13621 aagggggcga agtattcgaa acccccctct accgcaccct ggaagaagtc ccttttcccg
    13681 tggacctggc agtcctgcac atcccgcccc gattgattcc cgcggtcttg caggactgcg
    13741 tccggaaaag agtcaaaggg gtggtcatct ccgccgaagg gtttgccgag acgggagccg
    13801 aaggggcgaa gtatcaggaa gaagtgaaga gcatcctcca gtcctccggg atgaggggtt
    13861 tcggacccaa taccctgggt ttggtgaata cggcaacggg ccttacaacc tcttattatg
    13921 cgggccctcg gatgctccgt cccggcccga tcggttttgc ggcccaatcc gggatcttcg
    13981 tgggggcgct tctgcgctac ttgagctcct tcgaggggtt ccagctttcc aaggggatcg
    14041 gcctggggaa taaggtggat gtgaacgaat cggatgccct ttcctacctc atggacgatg
    14101 agcagaccca gatcatcggg atgtacctgg aggacgtccg ggatggaagg cggttcctgg
    14161 aaatcgccag gcaggcggta gccaagaaac cggttctcct gatcaaagga ggaagaaccc
    14221 gggagggagc tcgagccaca gcgtcgcaca ccgccagcat ggccgttgaa gatagcgtat
    14281 tcgagggggc cgtaaggcag gcaggggcgc tgcggctgac gggaatcgac gacttcgtcc
    14341 gggccctgaa gggtttttta aacatgcccc tgcccaaggg cggacgcctg gccttcgtga
    14401 cctacagcgg ggcccaggcc atcatgagca tcgatgcatc cgtagaccac gggctgcagg
    14461 tggcccgtct ggatgaatcc acccgggaaa ggattggccg ggtgattgct accgcttcca
    14521 agactcaaaa tccggtggat atttttccgg actggctggc caacggattt gaaaagacca
    14581 ccaccgaaat cctgaaagcc tttctggagg acgagggtgt agacgggatc atttgcatct
    14641 ccttcgccaa tgccggggcg gaaatctacc aacccatggt cgatatgctt aaaggagagc
    14701 gcactaaacc ggtttttttc tcccttctgg gggctaaaaa ggacatcgat gtgtgccaaa
    14761 acctcctgga agagaaccgc atcccctgtt ttgatttccc agaaatggcc gtccgggtct
    14821 ttgcccacat gtggaagtac gcgcagacta aacaaaaagc gcatagggca tagcgcattg
    14881 agcatcgcgt gaaaagttgg gaggaggtag gggaggcgcg cggcgcttgc ccttgcgcaa
    14941 gcggagaggg gaaaaacagc agaaaagagc cacgactcga aggtggaggg gaaaatgaca
    15001 aaggaagaga tgagcgaaaa ggcgatgcag atgatgatga aacggttcca ttgaagtcag
    15061 gcagttcttg cggtcgggca agaaaagctg gggagaaaag acgacgaagt catcaaggcc
    15121 atgggagctt tcggcggggg tctcggggga aatggagaag tctgcggggc cgttgccggc
    15181 gccctggcgg ttatggggct gaggttcagc cgtgcccggg cggatgaaaa agaagacccc
    15241 agaatgtggt cctatgccca ggaagtcctt cagcgattcc gggagattgt gaaaaaacat
    15301 ggggggatta cttgccggga gatcgccgga gtggactgga aagaccggga acaagccaaa
    15361 accttctata ggagtgaaaa agtcctggaa tgcgggcgga tcgtggggga taccacccgg
    15421 atggttggag aactactgga gcgtgcgggg taggcgcgac cggtcgtttg ctctcacccg
    15481 aaggagaatt ttaaacaaga acccggtacc gcggcgggga aatcgactca ctcttgcaga
    15541 ggggacaccg gctgggggta ttgagccggt cccttttctt gaaggaaaaa ccgcatttca
    15601 gacagacggc gggttccatt ttcaactggg ctgaccgtga tatatgctgc aagtggtcca
    15661 gaacgtcttt ctcccggatc cggaagacct gggaaatctc ccgcaaatcc atgggcccat
    15721 ccatcagggc cttggcgatc tctttcctca aggtctgcgg tttatctccc atttttttct
    15781 taagacgctt gagcccactt gaagcattta aggcacttct taggctctcg ccgccttccg
    15841 attttccagt gtttccatga aggcttccac ccgggtcttc acctgagcgt cggagtaaac
    15901 ccgggaatcg gtcatatcgg cttccaggac gaggctgggg gtgcctgttt tctccagaac
    15961 gattctttgg atatcatact ggccgaggga atagggcttg cagctccggt tggaatgaat
    16021 aaccatgccg tccacggaga acctcttcgt caggttgagg accttttcaa tcatgaggtc
    16081 gatgctgata ttcaggtaaa ttttcgtgta agcctgggaa aggccgtcta gggggtccag
    16141 ggtctcgaat ccttcaaaga cccatgcact ggtgtaggtg tcggccacga ggcaggctcc
    16201 aaaagaagaa aagagttcgg acagatcccg catcttgtac cagatgggga tattgtccca
    16261 caggaggcgg aatctctcct gcggcacggc gctgattccc agggcgatcc gttcctggag
    16321 ctcggctttc atgtcccggt agaagccggt cacttccttc gtcccccgca gagtgacaat
    16381 gggggccatg ttgatgaaag cgtcaaagca gctcatgggc gaggggcggt gggtacaaag
    16441 gtccagcacc tctttccaga gaagagccga ttcccccgaa ttgcgcagga cctcagtcaa
    16501 gcggtcccgg tcgaatttct ttctggtgat ggactccagg aagctgatca actcctcaaa
    16561 ttgctggcgg acataaatca ggctttccgg gacgacatgg tgatggataa aaggggtgtc
    16621 caggagaaag aggggaacgt tgaagatgcg gcttagggac tgataccatt tcatgactgt
    16681 cccgcagata ttgttgcagg ccagaaggaa gtccggcttg ggcaggcctc cggggatggg
    16741 actcttcttg gtcagtgccg atccaatgtc gccccgggcg taggaacaaa ggtcccggga
    16801 gtatcccaga ccttcggcga cctcgcaaag ctccacggac atgcgggatg ctcccagcat
    16861 ggccccatgg ttttccgggt agaccgggat gatgtccgct gcgatcaggg gttccaccgg
    16921 gcccccgctg gtgatccagg cgatcttctt gtccgtcccc tcggccattt tggcccccat
    16981 gtaataccgg gtcatgagtt ctttcatctt ctgagaagcg cgaagacgat tcttatcctt
    17041 ttctttcggg tccctcaatt cctgggccat cttattctcc cttgccggcg atgccgggat
    17101 aaggccgaaa ttcgaatttt gcctcccagc tgccccgaaa atattcattc ccggtcagcg
    17161 gctcaaagtc tcaacaaagg ctctgagtct cgtctccatg gctcccagcg gcaatcctcc
    17221 cggttcgatc tccaggagca ggctggggat cttctccttg ctcagcctct ccttcaggta
    17281 ggggtaatcg aaggcatggg ggtcgcagaa tttgagaagg aggaagacaa ccccctgggt
    17341 ttcggccgca ttcacccgct ccagaagccg atccgcacgg tcaatctcgg gattgaattt
    17401 gcaaggacag gggactttcc ggatcaatcg gtcggccaaa gcctcgatgg ggtcgccttc
    17461 gttgggaagg tcttcggcga agtacctcca acccgtgcag aggtcatccc ccacaagggc
    17521 cgctccgcaa gagtcaatga ggccatagat ggaagactga tcgcagacgc tccccaccaa
    17581 aaaaaggcgg actgagtgag gcgatggcgc ctgcccgttg cccaattggg agagaagctc
    17641 cttcatcctt gcattgtgct cgtctttggc catgaaattg gccgttttga taagggcgtg
    17701 cgagtctgcc gcggaaatcc agccaggata gctgctctgg taacgggaga cctcccgcag
    17761 gtaagagcgg ttctcattga acagatggaa agttccgcgg aggtctgcat cggagatcgg
    17821 atgaccgatg tactccgcca gacctccgcg aaagcggcgt agctcctgga tcaaataatt
    17881 gcgggcgctc tcggtgtgca gtttcaccgg caggaccaag tcccagtgga aggggaagca
    17941 aaggttctcc gcccagatgt cggacagacg ctggatggaa tcgcaggtat ggggaaatac
    18001 ggttccgtcc aaatagctca gttctccccg caggcctgac tccaggctgc tctggaccag
    18061 agagcagctg tagctctgga ggtgacggga ggctagggag atggtgtccc gggaactcag
    18121 aatccgcacg ggaaggaaac cagcggcctg aatcatttcc tctggggtgt tcgtacagaa
    18181 atagccgagg attttctttc cggtcttgtc tttccaattc ttgaggtaag cagaggggtc
    18241 tgctgcatat ccttttaacg ccccaaaagc attttgtttt tccataatcc gtcccttgaa
    18301 attttgcttg attttacaaa aaaaagacct tttcgtccat gataaaaagc gaaaagaaag
    18361 aggggcgggc aggggatgat gagaatcatc gccctctttg gcgatgtaag gttcaatgaa
    18421 cagaagaagg aattggaagg gattacaggc catgccgcaa aggcgcaagg cgcaggatgc
    18481 cgagggctca cccatgagtg ggaacgaccg gatgggcgca cgtttgtaaa ggcacgcggc
    18541 ttggtatcga gaccgagttc tgggaaaaga taaaactttc aggatttggc ctggaatttg
    18601 cagtcatatc ttcgcttgga taaaagagcc gattgatttc aataagccac ccaggaacct
    18661 gaacgatcca ctatttttga agttaaggga gtcaacgggt tgaggaccgg gtactttttc
    18721 atccctacgc tcttattcat tgccaatttt tacctataca actggatcgt ccgtttcacc
    18781 cagccctcgg tctgggtgcg gagggcgatt ctcctcgggc tttttctcct cctggcccta
    18841 ttccccgtca gcaggttgtg ggctgcctat gatttcaact ccttgaaccg ccttatcacc
    18901 ttccttgctt ccagctggat aggaatttca ttcctcctcg tcctctgtgc aggcggaacc
    18961 gacttgatcc gctttattct gcgtaaaaca gggctttccc caaaagtttc ctttttccag
    19021 ggtctttact accggcgagt cgcggtggct gcttccgtcg caggatgctt ggccttggga
    19081 ggctacggct tctgggaagc aggccatgtc ggggtgaccc ggatcgagat cccccttcgc
    19141 catttgcccc cggacctgga tggtctgtcg gtagttcttc tttccgatgt gcactatggg
    19201 atgctgcatg agaacggaag gctttcggac ctggtacggc gggtcaatga actccagccg
    19261 gacctggtcg tcttcaccgg ggacctggtg gatgcggatg tctcgcacat ggaagagatg
    19321 gccgggcctc tctcaggact gaaggctacc tacggtctat ttgccgtcat gggaaaccat
    19381 gaattctttg ccggggcaaa ccgggtcgag gcgatcatga aacaggcggg aatccaagtt
    19441 ctgcgcaacg aggtgcgggt tctcccgggg ggattgcagg ttctgggcat cgacgacccg
    19501 gccgttgttc gcagaggggg accgctaccc aattttgaag gacttatcag gggccttgac
    19561 gcccataaac catcgatttt gctctaccac cagcccatcc gtttcgaaga ggtggctgcg
    19621 gggaaagtag ggctgcaact aagcggccac atccacgggc cccagctatt gcccatggtt
    19681 cccttggcca ggctttttta cccccggatg cagggacttt ttcgcttgga ggacagctat
    19741 ctctatgtca gccgcggagt gggaaccggt ggccctccca tgcgcctggg ttccccgccg
    19801 gagatcgtgc atatccggtt gaggagcccg aaatcctcca tcttaaatca tgattcctaa
    19861 taggtcctga atcatcggcc gggcctgctc ctcgggcagg cagtattgca tctggagaaa
    19921 aggggaacgg aggcggctga tttcatcctt gaccagtcga tttttcaaga tcgcgtcggc
    19981 catcaatccg gccagctcgg caaaatcctt ttctttcatc ccgaagcggg tcatctcctg
    20041 gacacccgtc cgcaggccgc tactggcgga gaacccttcg tcgtcgggca gggcctggta
    20101 attgacgatc acattgtttt cttccaacct ccgggccgtc tccgggccga gggtgtatcc
    20161 cacccgcagg atcacctgat gggtctcggt gaaaccgacc ccggggtctc cctccacctg
    20221 aagcccctga tccttgaggg ctttggcgaa agccttggca ttggcgatga tctgcttctg
    20281 gtaatcgccg gcaaaggcgt tcatctcgta ggccgccagg aggagcccga ggagcgttcc
    20341 caggtggtgg ttgctgagac tccccggaaa agtccggcgg gtgatggcct tccacaggtc
    20401 gtaatattct gaggtctcgt ccaggttact ggcgatgatc ccccgctggg tgccgaagaa
    20461 agtcttgtgg gtggatcccg tgacaatgtc cgccccctcc cggaaggggt cctggaagtg
    20521 ggggccaatc aagcccagga catgggccat gtcgtataga atccaggtct tttctttcag
    20581 cctcgcaacc atccggcgca gctcggccag gggctcccgg tacaggacca tgctcttccc
    20641 caggatgagg agttcgggct tatgccggtg gatgagctcc tcggtctgtt ttaggtcaat
    20701 ctgataggga ttctcctcca gcacgggaaa attcaccgcc gccatccggt caaggtccgg
    20761 ggccacggag atgaaatccc gcagggcccc catgggttgg gagctgagat gcccgcccct
    20821 tcccaggtgg tggttcatga ctttgcgaaa acggcgtggg ttggtcttcc ggtagacgcg
    20881 gttcaggtaa tcgacgaagg agctgtaaac ggcggcattg gccatctgcc cgctgatgat
    20941 ccgggtctcc accatggtgc accccaggaa ccgggccatc tcttcctgca gcttgacctc
    21001 gacccattcg atgaagtccg tcccttggta atagaataca tcggcttccc ccaggaactc
    21061 gtagttccgg tgctccgcgt accgggagca ggggtcggag atggagagca gcttcaccag
    21121 cggagaaggg gtttgttcgg aggggatcag gttgatggtc tgacgctggc gccagagagt
    21181 gttctcccgg gccttccgca ccaggatttc agtggaaaag tgtagtggtt cccgggggcc
    21241 gggcctcttg atcaagatct cctcggctag caccgggcgc gcgtagggag gggcctcagc
    21301 gcccaaatgc cgcctgacca gcctgccggt gagcagcttt ccccgggcgg agatctttac
    21361 ctcctgttcc tccttcaggt ccgcgtccag ataaaccagg gcgatggatc ggcggccctt
    21421 ttcccggctg acccgggagg agattccctg cccctcgaat ttccagtagg ggaccatggt
    21481 cccgctggta acgtatccca cgggacggtc tcccgcaaag actgggaacc cctgccggac
    21541 gactccttcc gagagaacgg cgacgggcca gattcgccgc gggactaaag gggttgaggg
    21601 gatcaggtca ttccacccgt attcccgggc ctgaatctcc tgaagctgtt tccacaggga
    21661 ttgacgcccg aggtaatcgc ctttggattc cgagaagctg acggcgatct tggccggggt
    21721 cagggcgagg atggggatat cccggccctc ctggtctttc ccgtattcat gcccgtaaag
    21781 gggaaggccg gcttccaggc gcagcgtgtc tctggccccc agtccaatgg gaataacccc
    21841 ccgctcggag ccctggcgaa ggatcttttc ccagatgacc acggcgcgtt cggcggggaa
    21901 gaagatttca aaccccaggg gttctcccgt gtacccggtt cttcccacgt ccacgggggt
    21961 tccttcgatc caaactcgcc gttcattgtt cttccccggg tccggcagcc gggagccgct
    22021 cccctgcaag atggtttcca ggagcgcttt cgacagaggg ccctgcaagg agaacatggc
    22081 gatcctggcg gtgtggtttt ccatctccac tctggaaaat ttttttcgtt ggttctgcag
    22141 ccagtcccag tccttctggg tgttggcagc gttgaccacc aggcggtatt cttcttcatc
    22201 cagacgatag aggtaggcgt cgtcaaccgc cgtcccgttt tcgtcggaga tgatcgtgta
    22261 ttgagcctgc cccgggtcca gggccagggc gttgttagtc agggcatatt ggagaaaggg
    22321 gacggcaccc tctccggaaa tccgaaaccg gcccatgtgg gaaatgtcga agaggccgca
    22381 gaagcggcgg gtggtaagat gttcctccac gatcccgggt gggtagttga ggggcatctc
    22441 ccacccggca aattccacca tgtgggcgcc gtggctccgg tgccaggcgt tgagaggcgt
    22501 ctttttcggt tccgaggtca ttgcatcctc ctacagccgc agaaaagttt tttcattttt
    22561 tagtcgaagc taacaccgaa tacgggggat gtcaagaaag ggatggcaaa gaacaaattg
    22621 cagagattca gattttaaat ttccgacttg agatttagag acttttcctt ccctggaggg
    22681 ttgttcccat tcaaaaatag ggtctttttt ttcgtagaat acatccttca ccccatttag
    22741 agattttttt agaaagaaca taatattaat aattatcggg ggggtattcc gggccgcgag
    22801 ttgtgaatat tccaggcgcc gtttgagccg cctaaagtgg gttttgaatc aaaaaggggg
    22861 ccccacttga atctgaaaag gagggtaaag aagctatgac tgaatcgaca acggcaacag
    22921 gaacggtaac cagggaaaaa ttgatggaag acctgaaggc cgtggtgaac gacgcggaag
    22981 agctgttgaa ggccactgct aaccagaccg gggagaagat cgcgtcggcg cgcgccaagg
    23041 ccgaagagtc gttgaagacg gccaaagctc gaatcgccga ggaaggcaag gcggtgatgg
    23101 aaaaggccaa atcggccgcc aagtcaaccg atgacttcgt gcgcgaccaa ccctggaaag
    23161 cggtggggat tggagcgctc gccggcttcg tggtcggtct cttgatttcc cgccgctgat
    23221 ccgaatccat ggcctgggca gctcaggatc ccgtctcaag tcgccccgga ggattgttca
    23281 attcggtgaa agcgctggcg gccgctctcc tcgccatcgc ctacactcgg ttggaacttt
    23341 tttccgtcga gctggaagaa gagtgggtgc gcatcagctc cattctcacc tggagcctgg
    23401 tagcgctctt ctgcgccggt atcggtatcg tgtttaccac tctgttcctg gtgtacgcgc
    23461 tctgggggac ccatccattg gtggccctgg gcattccggc ggtccttttt ctcctgtttg
    23521 cagcgctggc ctggcggata gtggcggcca aggttcggac caagcctcgc atgttttccg
    23581 ccagcctcgc agaactggcc aaggaccgcg aagagttttt gaaatcagat tcatgaatgc
    23641 caaactgatt gagttagggg aacggcgggc caccctggtg tcccaagcgg cagtgcagcg
    23701 agaggagttg tcgcagacct tggctcattg gcgtgcgccc atatcgttgg cggaccaggg
    23761 ttgggcagtg ttccgatacc tcggaaagaa ccctttactg ctgggggggg cggtggcctt
    23821 tctggtcgcg cttcgcccct ggcgcatggc gaaatggttg ccgcccggtt ggctcttgtg
    23881 gcgtatcgcc cacaaagcac tcggaaccaa gggagtcatc cgcagcttca aatcttcggg
    23941 tggcggttcg cctgaggtct gcaataccca aacgtcgggc ggcgagcgaa aagaccaaag
    24001 gtaattccac cctatctttc attttccccc aagatcatcg attctgaatc ctcgcatgga
    24061 atccctcaag tcttggttcc tttttcccgc attctcttct tgatcttttt aggattccag
    24121 ggtaattctt tcaatattaa attcatggat tttcaaagag agagaaacgg gggcccatac
    24181 cggaattccc gcccttgttg ggcatttcag tccagttggg gaaggcacca agaaccacgt
    24241 ggtttcaggg cgccctgcat tcaaggtagc gcaaggaaca atgaggaatg aatacgcagg
    24301 aatagctcgg gccccttttt tctatcttta cccgctttta atttaaggca cttaatcaca
    24361 cttaaggcac ttgagggact gtactggagg aaagctatca agggagcttc tatgaaatac
    24421 gatttcgaca tgattc
//
LOCUS       DASPRR010000088         5708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_25586, whole genome shotgun sequence.
ACCESSION   DASPRR010000088 DASPRR010000000
VERSION     DASPRR010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25586"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1102
                     /locus_tag="VEL68_03110"
     CDS             380..1102
                     /locus_tag="VEL68_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HXZ34987.1"
                     /translation="MDSSEPKIGQRFQQETKYYRPSYRSKLSERVPEVSPSRTISLPP
                     PALDGGPSLWETLRERRSVRDYSALPLSLKDLSNLLWATQGITEKAFAPWYRTAPSAG
                     ALHPIDTYLVVNRVEGMEPGIYFFEVVEFSLTLKRAGDFSGPMAQAALNQDIAQSAAV
                     VFVWVAVIQRSRQKYRQRAYRYIYLDCGHIGQNLYLATTALGLGCCGIAAIFDDEVND
                     LVGVDGQEETAIYLAAVGKKEK"
     gene            complement(1143..1721)
                     /locus_tag="VEL68_03115"
     CDS             complement(1143..1721)
                     /locus_tag="VEL68_03115"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXZ34988.1"
                     /translation="MESSFVVFQTKLGWMGLVGDEKGVQRIYLPEPSREDLVERITRE
                     FPNGREGDQALAEAKKEILEYLDGCRKKFEIPLDLSWATPFQQKVYQTMLAIPFGRVQ
                     TYRWLAEKIGNPKGLRAVGSANGKNRCPLVVPCHRIVGSDGRLTGFSAPGGLELKAQL
                     LRLEGVPVEKGRVKPLGNSVGEMGNKKNGKKK"
     gene            complement(1875..2126)
                     /locus_tag="VEL68_03120"
     CDS             complement(1875..2126)
                     /locus_tag="VEL68_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34989.1"
                     /translation="MKIIREIFCTKCGKKQVTDVKDLLESDLFWRVCEFCKSFGLKIV
                     TRRKATFREYIEAFYNTLGFRVYQMEAFIKDLLKDRTSL"
     gene            complement(2332..2691)
                     /locus_tag="VEL68_03125"
     CDS             complement(2332..2691)
                     /locus_tag="VEL68_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXZ34990.1"
                     /translation="MAILFFLLGFFLFLDCDSFAQTYKYVDKKGTICFTDNPLSFPSK
                     DVSNEEPKTKQVTLQKGRAKPEIKDIFQLGQEMLEKELAKPPEKQDRRLIKELGENLY
                     GDISGQKDKEAQASSKR"
     gene            complement(3188..4843)
                     /locus_tag="VEL68_03130"
     CDS             complement(3188..4843)
                     /locus_tag="VEL68_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ34991.1"
                     /translation="MERPWLKFYEKGVPHHIEYPRVPLYRVLDETARDFPALDAVIFR
                     GKRIKYGELAGWARDLASALHEIGIKKGQRVAIMLPNCPQYIVAYYAILRLGGVVVNV
                     NPMYVERELEFQLHDAEAQAIVALRDLLPRLESVKEKIPLKTMILTDLDEHVSATGRK
                     APAQGLGPGVYEYAELLEKGKTQVPPVVSVDPDEVALLQYTGGTTGFSKGAMLTHFNL
                     VSDVVQCVSWNVGAQRGEERMLAVLPFFHVYGMTVTMNEAIYLAATIILLPRFQVDDC
                     LEAIKQYQPTRFPGVPTMYIGIINHPRVKEYNISSIKVCSSGSAPLPVEALKKFEELT
                     GGKISEGYGLTEASPVTHANPFSGKRKVGSIGLPRPDTDAKVVDLETGEKDLPPGEEG
                     ELCIRGPQVMKGYWNRPEETARSLRKGWLYTGDIAKMDEEGYFYIVDRKKDMIICGGF
                     NVYPREVEEALYLHPKILEAAVLGVPEPYRGETVKAFVVLKAGQKATAEEIIEFCRQN
                     LARFKVPTQVEFRQDLPKSHVGKVLRKVLREEEAKKESSHRPS"
     gene            complement(5282..>5708)
                     /locus_tag="VEL68_03135"
     CDS             complement(5282..>5708)
                     /locus_tag="VEL68_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34992.1"
                     /translation="LTPDEVYLPAPYERHKTHQKVTRLALDALRSSIASKPLLLGYSL
                     WGGFLGGKKRWVKDISPFIRKKVEAVLAHSTQIAYKNYQQGILGRNNADAIFWESHDI
                     QKATFVEIFLDMTELLERKNLSPDSFIREDVESFILAFL"
BASE COUNT         1255 a         1568 c         1400 g         1485 t
ORIGIN      
        1 tcgtgcttgt tggggttcag ggtggaggaa tttggatcat caggctcggc gggtgcttat
       61 ctttccatga aacccctccc tatgttccgc agggagaggc ctgagaggag attcctccaa
      121 gcagaaaaga gaaccaaagc aattttccat ttcaattccc ccctaggaca ggcccgatcc
      181 aatggaagaa ccaccgcctc aggatgatga tccaaattcc tccaccctga accccatggg
      241 atcccccctt ctttccaggg atcttatttt cgaaggactc ttttttaaga actgggggtg
      301 ggcaaggtga ttgatatagt tgacagtttc ttgggacaat tttaaactct ttttattatc
      361 cttctgcggg aggtcggtta tggattcttc ggaaccgaaa attgggcaac gcttccagca
      421 ggaaaccaaa tactaccgcc cttcctatcg atccaagctg tcagaaagag tcccggaggt
      481 ctctccttcc cggacgattt ctcttccccc tcctgccctc gacggcggcc ctagcctatg
      541 ggagaccttg cgggaaagac gttccgtccg ggattacagc gcacttcccc tttccctaaa
      601 agacctttcc aatcttctct gggctaccca gggaatcacc gagaaggcct ttgccccttg
      661 gtaccgcacg gccccctcag ccggcgccct ccatcccatc gacacctatc tcgtcgttaa
      721 ccgggtggag gggatggagc cggggattta tttctttgag gtggtggagt tttccttgac
      781 cctgaagagg gcaggagatt tttccggacc catggcccag gcggccctga accaggatat
      841 agcccagtcc gcggccgtgg tttttgtctg ggtggcggtc attcagcgtt cccgccagaa
      901 gtaccgccaa cgagcttacc ggtacatcta tctggactgc ggccacattg gtcaaaatct
      961 ctacctggcc accacggcat taggtctagg ctgctgcggg attgcggcaa ttttcgacga
     1021 cgaggtgaac gacttggtgg gagtcgatgg gcaagaggaa accgccattt atttagcggc
     1081 ggtgggaaag aaagaaaaat gacggcgttc tgccctattt tcttttcttc tcctactccg
     1141 agtcacttct tcttcccgtt ttttttattc cccatttcgc ctaccgaatt cccaagaggc
     1201 ttgaccctgc ctttctccac aggcacccct tccagtctta acagctgcgc tttcaattcc
     1261 aggccgccag gggcggaaaa accggtcagg cgtccgtcgc tgcctacgat ccggtggcaa
     1321 ggaacgacca ggggacagcg gtttttcccg ttggcgctgc ctaccgctcg aaggcccttt
     1381 gggttcccga tcttttccgc cagccagcgg taggtttgga cccgcccgaa aggaatggcg
     1441 agcatggtct ggtaaacttt ttgctgaaag ggagtggccc aagagaggtc caggggtatt
     1501 tcaaatttct ttctacaccc gtccaaatat tccaggattt cctttttagc ctccgccagg
     1561 gcttggtccc cttcccggcc gttgggaaat tctctggtga ttcgttcaac caaatcttcc
     1621 cgggagggct caggcaggta aatccgctgc accccttttt catcccccac caaccccatc
     1681 cacccgagtt tcgtctggaa aacgacaaag gaactctcca tcttcctccc tccggctcat
     1741 gcattttcca acctaccagt acaaaaaata taggccaatc caattttctg aattttccaa
     1801 cttaaaaaag aagggcggat cattgaccgc ccttcctttt tccaggattt ctctccccga
     1861 tgggaataaa atacttacag tgaagtccta tccttcaaaa ggtccttaat aaaagcttcc
     1921 atctggtaaa ctctaaatcc tagggtgttg taaaaggcct cgatatattc ccggaaggtc
     1981 gctttacgcc gggtgacaat tttcagcccg aagcttttgc agaactcgca tacgcgccag
     2041 aaaagatcgc tctccaagag gtcctttaca tcggtaacct gctttttccc gcacttggtg
     2101 cagaatatct ctcttatgat cttcatctgc ccattttcct gaatccgcaa gagacgcaaa
     2161 ttctcctcga tggaaatatt ttaggatagc cggattcgaa tgtaaatcag aattttccaa
     2221 caggagtcca ggaatcgtcc ctccggcaga aagggaagag gcggtcatgc agaatccata
     2281 ggtgcggagt tctttaaatc ggcagggaga aaggcgtttt ctttgaaaac ctcatcgttt
     2341 tgaggatgct tgcgcttctt tgtccttctg tccggatata tctccgtata aattttcccc
     2401 taattcctta atcaaacgac gatcttgttt ttcgggaggt tttgctaatt ccttttcgag
     2461 catctcttgc cccagttgga atatgtcttt tatctctggt ttggctcttc ctttttgcag
     2521 ggttacctgt tttgtcttgg gttcctcgtt ggaaacatct ttggagggga aagacagtgg
     2581 gttatccgta aagcatatcg tacccttttt gtccacatac ttgtaagtct gagcaaaaga
     2641 atcgcaatct aaaaaaagga aaaagcctag gagaaaaaac aatatggcca tcccccacct
     2701 ccggtttctt tcatccctct acccaaaatc ggcatggcat taagttcccc cgtcctatca
     2761 cgcatgacga gggctacttc tttctttgtt tttgaaacaa tttaaaaata ctcaagaatc
     2821 atgccagaaa atgaaacttg ccgtaacttt cccttaacta ttgaatatca ttcgtctaac
     2881 tattgatgtt caagacctac gcttttctct cccgagaaca tttttcgttc agaattgtaa
     2941 gatgattagc gatttccaat cttctgccag tcctcttcgt gcaaaattca accgaactta
     3001 aacaccgcat tgatccatgt caagggggag gatttagggg acggcactcg agggaaagca
     3061 atcttagggt cccttcttgt taatgatgag ggattttccc agggatggta gaatatctct
     3121 tcttttttgg atgcggcagc tccggaaacc aaaaggttgt ccgaaggcca atgaatttcc
     3181 cgagcgatca gcttgggcga tgggagcttt ccttcttcgc ctcttcttcc ctgaggacct
     3241 ttcgaagaac cttccccaca tgggatttgg ggaggtcttg gcgaaattcg acctgggtgg
     3301 ggactttgaa acgggccagg ttttggcggc agaattcgat gatctcttcg gcggtggctt
     3361 tctgccctgc cttaagaacg acgaaagctt tcaccgtctc cccgcggtag ggctccggca
     3421 cccccagaac tgcggcttcc aggattttgg ggtggagata gagagcttct tcgacttccc
     3481 gggggtaaac gttgaacccc ccgcaaatga tcatgtcttt cttgcggtcg acgatgtaga
     3541 agtatccttc ctcgtccatc ttggcgatat cgcctgtgta aagccagcct tttcgcagcg
     3601 atcgcgccgt ctcttccgga cggttccaat atcccttcat gacctggggt ccgcggatgc
     3661 agagctctcc ttcttccccc ggaggaaggt ccttctcccc agtttccagg tccaccacct
     3721 tggcgtcggt gtccggacgg ggaaggccga tgctccccac ctttcttttt ccagaaaaag
     3781 ggttcgcatg ggtcacgggt gacgcttcgg tcagcccgta accttcgctg attttccctc
     3841 cggtcaattc ctcgaacttc ttcaaggcct caacgggcaa aggggcggaa ccggagctgc
     3901 acaccttgat ggaggagata ttgtattcct tgacccgggg gtggttgatg atgccgatat
     3961 acatggtggg gacccccgga aatctcgttg gctggtattg tttgatggcc tccaggcagt
     4021 cgtccacctg gaaacgggga agcaggatga ttgtagccgc caaatagatg gcctcattca
     4081 tggtcaccgt cattccgtac acatggaaaa agggaaggac ggccagcatt ctttcctccc
     4141 ccctctgcgc gcccacgttc cagctcacac attggaccac gtccgatacc aggttgaaat
     4201 gggtcagcat ggctcctttg ctgaatccgg ttgtgccccc ggtgtactgg agaagggcca
     4261 cttcatccgg gtctactgaa accacgggcg gtacctgagt ctttcctttt tccaaaagct
     4321 cggcgtattc gtacaccccc ggcccgagtc cctgcgcagg agcctttcgc cctgtcgcgc
     4381 taacgtgttc atccaggtcc gtgagaatca tggtctttag ggggattttc tccttcacgc
     4441 tctcaagccg gggaagaagg tcccgcaggg ccacgatcgc ctgagcctcc gcgtcatgga
     4501 gctggaattc cagttcccgc tccacataca tggggtttac attgaccacc accccgccga
     4561 gtctgaggat ggcgtaatag gcgacaatat attggggaca attgggaagc atgatcgcca
     4621 cccgctgccc tttttttatg ccgatctcgt ggagggcgga ggccaggtcc cgggcccacc
     4681 cggctagctc gccgtacttg atccgcttgc cccgaaagat gaccgcgtcc agggcaggaa
     4741 aatccctggc cgtttcgtcc agcacccgat acagggggac ccggggatat tcgatatgat
     4801 ggggcacgcc cttttcatag aatttcagcc acggtcgttc catgggattt ctcctcaccc
     4861 aagatgaaaa tgaagattat tctggagtgt cgtaccctca cccctgcccc ttccttttat
     4921 agaagatttc ggaccttcca tagggacgcc tcgcgcgcca aaatcatgac ctccggcaga
     4981 accggaggta tgaaaagcaa tgaaccgctg aaagcggatt gaaaagggcg ccacctaaag
     5041 ggggccttga tttggtgcca ggctcggggg cacaaacgaa gcatcccgaa atgtcaatcc
     5101 gcctcaggcg tacccccggt agagccgagg gtttaccata atgaaatttt tcaatcccat
     5161 ttttcagttc ggcggtttct gtctttttaa ttccgcgctc cgaattccgc tatgcgcaat
     5221 tcctaatgtc ttgacccgga tcatcctcaa aaatcggagg tttgcggcga agcgccccat
     5281 tttaaaggaa agccaggatg aagctctcca catcctcccg gatgaaacta tccggggata
     5341 aattttttcg ttccaacagc tcggtcatgt ccaaaaaaat ctctacgaaa gtggctttct
     5401 ggatgtcatg gctttcccag aaaatggcat cggcgttgtt tcgtcctagt atcccctgct
     5461 ggtaattctt gtaagcaatc tgggttgaat gagcgaggac ggcttccact ttctttcgga
     5521 tgaacgggct tatatctttg acccaccgct tttttccgcc caaaaagcct ccccagaggc
     5581 tgtatcccaa gaggagaggt ttcgaggcga tcgacgatcg cagggcgtca agcgccaggc
     5641 gggtgacctt ctggtgggtc ttatgccgtt cgtagggagc gggcagataa acttcatcgg
     5701 gcgtcagc
//
LOCUS       DASPRR010000089         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_673674, whole genome shotgun sequence.
ACCESSION   DASPRR010000089 DASPRR010000000
VERSION     DASPRR010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673674"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VEL68_03140"
     CDS             <1..189
                     /locus_tag="VEL68_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34993.1"
                     /translation="RTGRILDPQEGLGGRARGGSQRNEEEIKKNPSVEGHREILRVPK
                     KNLPKPNFILLSKRGVVK"
     gene            479..1807
                     /gene="thiD"
                     /locus_tag="VEL68_03145"
     CDS             479..1807
                     /gene="thiD"
                     /locus_tag="VEL68_03145"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXZ34994.1"
                     /translation="MFPRVLTVAGSDSGGGAGIQGDLKTISLLGGYGMSAITALTAQN
                     TRGVQSIHEVPAEFVGLQMDSVISDIGVDAMKTGMLGNSSIIRVVSRKVRRYRISQVV
                     VDPVMVSKGGVRLLSPEAEETLKKELIPLALIFTPNIPEAEVLTQDRIRGVEGMRRAA
                     KQVLRMGARNVLIKGGHLIGDPVDVFFDGRSFSEFSGPRLPSLHTHGTGCTLSAGIAL
                     ELARGNSPKAAVEKAKSFITSAIQFSFPLGQGIGPVNPYTPAIRDGERYQVIHSLRKA
                     FHRIQEKKVGHLFPEVQSNLGYALSMAQGPEDVAAFPGRFVRWGKEVVRIADPEFGAS
                     QHIAKIILTALRYNPEMRSAMNLRFSDDLLTRARKAGLSLGHFSRRDEPAWVKRREGS
                     SLSWGVEQVLRKTKKMPDLIYDRGDVGKEPMIRVLGRDPLEVVKKVLRLA"
     gene            2100..3470
                     /gene="cdaA"
                     /locus_tag="VEL68_03150"
     CDS             2100..3470
                     /gene="cdaA"
                     /locus_tag="VEL68_03150"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HXZ34995.1"
                     /translation="MTYVFYRLYVWIQGTRALRILIALLALGIFYPLARWSGLFLTSW
                     ILQYLWAVILVIIVVVFQSEIRQVLDRMSPVRFFLGRPETFDRIIVEEVTKTAFDLAQ
                     RRIGALIVFKRQDMLEDYLKGGVPLDGRVSHEVLSSIFLPQSPTHDGAVVIQGGRIIC
                     MGCYLPLSDNTGLPQKYGTRHRAGIGITERCDAIALIVSEERGEVSLTVKGEIERIDS
                     PEELKTRLESTIFKPQQDKNAWPTAFTDNFVPKIVSFLLVCILWVFIGGQPRAEIWMT
                     IPLEFRNMPSNMEIVGELINRVEIAIRGPRTLISSMTPDQLKAHVDLSRSQAGINNIR
                     LAPENVRVPLGTEITKINPSMVRIRLEDIKSRSIPVKAHLVGRLPRPFRLKSVQVEPP
                     EIVIQGPAVNLKKVREIFTEPVDLGEVKDDAAISVPVDLNLPQLRLAPDQPSQVTIFL
                     RLEKET"
     gene            3487..4257
                     /locus_tag="VEL68_03155"
     CDS             3487..4257
                     /locus_tag="VEL68_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ34996.1"
                     /translation="MGDIYSEMTAVSQKIAASFPPLRFYTCCRQSLLLSQSLFSEDPR
                     ISKCRAIVHQELKDNLGHGLDHVQKVAVEAGALLFLEAEKLMMGEPGKEQAGILVQMA
                     GLLHDLCREEKDHARLGALAAGKILREFSFTPGEKRMIVEAIANHEAFLEPQPIESPL
                     GQLVSDVLYDADKFRWGPDNFTLTLWEMLRFSQAPMVRLIHRFPKGMKGIARIKETFR
                     TETGKTYGPEFIDQGLQIGEKVLEFLQERFAAELAQGK"
     gene            complement(4322..5491)
                     /gene="dinB"
                     /locus_tag="VEL68_03160"
     CDS             complement(4322..5491)
                     /gene="dinB"
                     /locus_tag="VEL68_03160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXZ34997.1"
                     /translation="MNRKIIHIDMDAFYASVEQHDNPALQGLPVIVGGDPGRRGVVSA
                     ASYEARVYGVHSAMPSSQARRLCPQGVFLPVRMHRYREVSDQVFQILREYTPLVEPLS
                     LDESFLDVTGSEKLFGTALQIAMEVKRRIRQATGLTASAGIAPNKFLAKIASDYKKPD
                     GLVEVKPEEVQVFLRDLPISKLWGVGKSTEEVLKGMGILKVGQLAVFPAEKIERKLGK
                     FGLELIALARGEDERPVSPESEAKSISQEETFTPDLEHLEQMKKVLLDQAEQVGWELR
                     KQKLKGSTVTVKVRYPDFSLATRSQTLSSPTDQGIEIYRTALKLLSRTEALQRRARLL
                     GVGISHLTHRDAPEQYLLFDSGGKKAEHSIQAMDRIWERFGPQAIKRATLVDKKK"
BASE COUNT         1240 a         1587 c         1613 g         1215 t
ORIGIN      
        1 cgaacggggc ggattctcga ccctcaggaa ggacttggcg gaagagcaag aggcggcagc
       61 caaagaaacg aggaagagat aaaaaaaaac ccttctgttg aaggtcatag ggaaatcctc
      121 agggttccaa aaaaaaatct acccaaacct aattttattc tactttcgaa acgcggggtt
      181 gtcaaatagg gaatgagctt tcccacctgc cccagaaaat gaatcccgcc ttatggttgt
      241 tgcgcgctat ccgcctatgc cggactttcg ggcctggcaa cctgggaaat tctacccatc
      301 ttacgcctac tcctctctcc ccattgcctc tcgacaaagt cctcatgaat tttttccggc
      361 cttggtttta ttatcctcgg aaatgagcta caatgcccaa aaccactcca aagcccgagt
      421 agccgatcgg ccgatatctt caggcacaac ggggcgagtg ggcaggagga aaggaatcat
      481 gtttccgcga gtattgacgg tggccggatc ggattccggg ggcggcgccg gaatccaggg
      541 ggacctgaag accatctccc tgctgggggg atacgggatg agcgccatca ccgcgctgac
      601 cgcccagaac acccggggag tccagagcat ccatgaggtc ccggccgaat ttgtgggcct
      661 gcagatggat tcggttatct cggacatcgg agtggacgct atgaagacgg gcatgctggg
      721 caactcgagc atcatccggg tggtcagccg gaaggtccgc cgataccgga tctcccaggt
      781 ggtggtcgat ccagtcatgg tgtccaaggg gggagtgcgg ctgctttcgc cggaagcgga
      841 agagactctc aaaaaagagc tgatccccct ggccctaatt ttcaccccca acatcccgga
      901 agctgaagtc ctgacccagg acagaatccg gggagtggag ggcatgagaa gggcggcaaa
      961 gcaggtcctc cgcatgggcg cccggaatgt gctcatcaag ggggggcatc tgatcggaga
     1021 cccggtggat gtcttttttg acggaaggtc attttccgaa ttttccgggc cacgccttcc
     1081 ttccctccat acccatggaa ccggatgcac gctctccgcg ggcatcgccc tggaattagc
     1141 cagaggaaat tcgccgaagg cggccgtgga gaaggccaag tctttcatta cttcagcgat
     1201 ccaattctct tttcccctgg gacagggcat cgggccggtg aatccctaca ccccggcaat
     1261 ccgagacggg gaaagatatc aagtgatcca tagcctgagg aaggcctttc atcgaatcca
     1321 agagaaaaaa gtaggccacc tttttccgga ggtccagtcg aacctgggtt atgctctttc
     1381 catggcccaa ggcccggaag atgtggccgc ctttccgggg aggttcgtac gttggggaaa
     1441 agaggtggtc cggattgcgg acccggaatt cggggcctcc cagcatattg ccaagatcat
     1501 cctgaccgcc ctgcgctaca acccggagat gcgctcggct atgaacctgc gtttttccga
     1561 cgacctcttg acccgggcaa gaaaggcggg tctctccttg gggcatttca gccgcaggga
     1621 cgaaccggcg tgggtgaagc ggcgggaggg atcatccctt tcctggggag ttgaacaggt
     1681 tttgcgaaaa accaaaaaga tgcccgacct catctacgat cggggggatg tgggaaagga
     1741 gcccatgatc cgggtcctgg gacgggaccc actggaggtg gttaaaaaag ttctccgcct
     1801 ggcctgaaag tccggcggag aaattcggaa tggggaacac catttccaag aaagttgcgg
     1861 gttgctgttt actcgttgct cgttaaagaa gaaaaactaa aaactcagcc aggaaccagc
     1921 gaccaggaac ccgcaacgag caaccagcaa tgttttttca ttccccattt gaaaggtgga
     1981 tttcagcccc ggcatgtatt aagatagatt atctcaagcc tcccgggagg tgaaagattc
     2041 aatacctgaa agaagccctc ctgagcatcc gctggcagga tgtcatcgat atccttctga
     2101 tcacttatgt tttctaccga ctctatgtat ggattcaggg aacccgggcc ctgcgaatcc
     2161 tgattgccct tctggccctg ggcattttct accccctggc ccgctggtcc gggcttttcc
     2221 tcactagttg gatcctccaa tatctgtggg cagtcatcct ggtcatcatt gtcgtggttt
     2281 ttcaatcgga gatccgccag gtcctggacc ggatgagccc ggtacggttc ttcctgggtc
     2341 ggccagaaac ctttgaccgg attatcgtgg aggaggtgac caaaaccgct ttcgacctgg
     2401 ctcagagacg catcggagcc ctcatcgttt tcaaaagaca ggatatgctg gaagattatc
     2461 tcaaaggggg ggttcccctc gacggccggg tgagccacga agtcctctcc agcatctttc
     2521 tcccccagtc tcccacccac gatggggcgg tagtcattca gggaggaaga attatctgca
     2581 tggggtgcta ccttcccctt tccgacaata ccgggctgcc ccagaaatac ggaacccgtc
     2641 accgagccgg gatcgggatc accgaacgct gcgacgccat cgcgctcatc gtctcggagg
     2701 agcgggggga agtatctctg acggtgaaag gagagatcga gcggatcgac agccccgagg
     2761 agctcaaaac ccgtctggag tctacgatct tcaaaccgca gcaggacaag aacgcctggc
     2821 cgactgcctt caccgataat ttcgttccca agatcgtgtc tttcctcctg gtctgcattc
     2881 tctgggtttt catcggcgga cagccgaggg ccgaaatctg gatgacgatt cccctggaat
     2941 tccgcaacat gccctccaac atggaaatcg tcggagagct gatcaaccgg gtggaaatcg
     3001 caatccgcgg tccccgtact ctcatctcct cgatgacccc ggatcaatta aaggctcatg
     3061 tggacctttc ccgctcgcag gcggggatca acaacattcg cctcgctccg gaaaatgtcc
     3121 gggttcccct ggggacggaa atcaccaaga tcaacccgtc tatggtccgt atccggctgg
     3181 aggacatcaa gtcccgttcg atccctgtga aggcccacct ggtgggaaga cttccccggc
     3241 ccttccgcct gaagtcggtt caggtggagc cgccggaaat cgttatccag gggcctgcgg
     3301 tcaacctgaa gaaagtccgt gaaatcttca ccgagccagt ggacctgggg gaggtgaagg
     3361 atgacgctgc gatctccgtc cccgtggacc tcaatctccc ccagcttcgt ctggctccgg
     3421 atcagccctc tcaagtgacg atatttcttc ggttggaaaa ggaaacttga ttccggagga
     3481 gaggccatgg gagatattta tagcgaaatg accgccgtct cccagaagat tgccgcttcc
     3541 tttcctcctc ttcgcttcta tacctgctgc cgacagtcgc tcctgctatc ccaatccctt
     3601 ttttccgaag acccacggat ttcgaaatgc cgggcgatcg tacatcagga attgaaagac
     3661 aatctggggc acggactgga ccatgtgcag aaggtcgcgg tagaggccgg agccctcctc
     3721 ttcctcgagg ctgaaaagct catgatggga gagccgggga aagagcaggc ggggattctg
     3781 gtccagatgg ccggtcttct ccatgacctt tgtcgtgaag aaaaagatca tgcccggctg
     3841 ggggccttgg cggccgggaa aatcctccga gaattttcct tcactccggg agagaaacgg
     3901 atgatcgtgg aggcgatcgc caaccacgaa gcttttcttg aaccgcaacc gatcgaatcc
     3961 ccccttggcc agttggtcag cgatgtcctc tatgacgccg acaaattccg ctggggaccg
     4021 gacaacttca ccctgactct ctgggagatg ctccgttttt cccaggcgcc catggtccgt
     4081 ctgatccatc ggtttcccaa ggggatgaaa gggatcgccc ggatcaaaga gaccttccga
     4141 accgaaacgg ggaagaccta tgggcctgaa ttcatcgacc aggggctgca gatcggggag
     4201 aaggtcttgg aattcttgca ggagaggttc gcggcggagc ttgcccaagg aaagtaaaga
     4261 tggggagatg gggagataag gggtgaaaat tcactcccca tctcccgatc ccctgagctg
     4321 ttcatttctt tttgtccacc agggttgccc tcttgatggc ctggggcccg aacctctccc
     4381 agatccgatc catggcctga atggaatgtt ctgctttttt gccaccggaa tcgaatagga
     4441 ggtactgctc aggggcatcc cgatgggtga gatgggagat gcccactccc aggaggcggg
     4501 cccttctctg gagggcctcg gtgcgggaca ggagtttcag ggcggtccgg tagatttcga
     4561 ttccctggtc cgtgggggaa gagagggttt gacttctggt ggccaggctg aaatccgggt
     4621 accggacctt caccgtaacc gtggagcctt tcaatttctg tttccggagc tcccagccta
     4681 cctgctcggc ctggtcaagg aggaccttct tcatttgctc caaatgctcc aggtccggag
     4741 tgaaggtctc ctcttggctg atggactttg cttccgactc gggagagacc ggccgttcgt
     4801 cttctccccg cgccaaggcg atcaactcca ggccgaattt tcccagtttc ctttcgattt
     4861 tttccgcggg gaagaccgcc agctggccga cctttagaat ccccatcccc ttcaatactt
     4921 cctcagtgga cttccccact ccccatagtt tggagattgg aaggtcccgg aggaagacct
     4981 ggacctcttc gggtttgacc tccaccaggc cgtcgggctt tttataatcg gaggcgatct
     5041 tggccagaaa tttattcggg gcgatcccgg cggaggcggt gaggccagtg gcctgccgga
     5101 tgcggcgttt gacttccatg gcgatctgca aagcggttcc aaaaagtttt tcgctgccgg
     5161 tcacgtccag gaaggattcg tccaaggaaa gaggctcgac gagaggagtg tattcccgga
     5221 gtatttggaa aacctggtcg gagacttccc ggtaccggtg catccgcacg ggtagaaaga
     5281 ccccctgggg gcagagtctt cgggcctggg aagaaggcat ggctgaatgg acgccataga
     5341 cccgggcctc ataagaggcg gcggaaacga ctcccctcct cccgggatca ccccccacga
     5401 taaccggaag cccttggaga gccgggttgt catgctgctc caccgaggca tagaaggcgt
     5461 ccatgtcgat gtggataatt tttctattca ttgaggagtc gggtcgcgaa gtcccctagt
     5521 cgcacagtcg cgccgtcaat gccccgtctc gtttggtcca cctgccataa aatccctttc
     5581 gagagtgatt cacgtacccg gagcagaaat aagcattccc aattccaaag agcgggggag
     5641 gggccaatca acatt
//
LOCUS       DASPRR010000090         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_673988, whole genome shotgun sequence.
ACCESSION   DASPRR010000090 DASPRR010000000
VERSION     DASPRR010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673988"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..2203)
                     /locus_tag="VEL68_03165"
     CDS             complement(56..2203)
                     /locus_tag="VEL68_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ34998.1"
                     /translation="MSTNGESNRDDRVVTSTCSFDCGSRCLLKVHVSEGRVQKITTDD
                     QPGPGLKACARGLAQRDVLYAGDRLKVPLRRAGERGSGEFEPISWEEALDQVARELAR
                     VKDQFGPQSVFLMDYFGSMSPLHNTGRTGRRFFSLFGGCTTWWGNTSAEAAIFSSLMT
                     FGTFFTRNSRDNLLHSKLILLWGWNPLETRFGPDTAYYLSEAKKKGARIICVDPRLSP
                     TGKSLADQWIAIRPATDTGLLVAMAQVMITEDLYDRRFIETYTSGFDKFREYVMGEED
                     GIPKNPAWAEEITGVPSEVIVRLARDYALLKPAALYAGWAPGRTAYGEQYHRAASTLA
                     AMTGNIGVVGGHVSGGTDRMALGILRKGLPVPESQNPMVHVSELYDLLLRGKEGRFPS
                     DIKVLYILGCNLLNQWLNTNKGVKALQKPELVVVHELFMTPTARHADIVLPVAHFLER
                     QDVGQPWTGGPYNILMEKAVEPLPGVKSDLDIFSELASLLNLAGYNDKTNEEWLQEFL
                     SDTPGLPDYPRFKGEKVHSYEVRKPWVAFQEEIEDPLHHPFRTPSGKIEIHSRKIAEM
                     NDPMIPPIPKYIEPWEGPKDPLAGKYPLQMVSPHSKGRVNATLDNIPRLKAIADDDLW
                     LNRGDAETRRIQPGEKVRVFNDRGRMVRKAKVTDRIMPGVVSLEAGSWYDPDEKGVDR
                     GGCVNVLTVDKKSPAGSFPCNSCLVEVEGEKEP"
     gene            complement(2200..>2458)
                     /locus_tag="VEL68_03170"
     CDS             complement(2200..>2458)
                     /locus_tag="VEL68_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXZ34999.1"
                     /translation="DALTGRGYGVAFFSPFDTDRYSFPWAPIKISPIGLKSFFSSWGT
                     EVILSEVRWVWVPAILLLLVVRAFRLMRSRGLLRMSRGFEE"
BASE COUNT          505 a          737 c          694 g          522 t
ORIGIN      
        1 tcctatccta ctactttccc ccggaatcca ttaatgatct gggtcaggga aaaaatcaag
       61 gttctttttc tccctccact tcaaccaggc agctgttgca ggggaaggat ccggcgggcg
      121 atttcttatc caccgttaga acattcacgc agccgccccg gtctacccct ttttcgtcgg
      181 ggtcatacca ggaaccggcc tccaggctca ccaccccggg catgatcctg tccgtcactt
      241 tcgctttcct caccatccga ccccgatcgt tgaataccct aaccttctct ccgggctgaa
      301 tcctcctggt ttccgcatcc cctcggttga gccacagatc atcatcggcg atcgccttga
      361 gcctagggat attgtccaga gtggcgttca cccttccctt ggaatgaggg ctcaccatct
      421 ggagcggata tttgcccgcc aaaggatcct tcggcccttc ccagggctcg atatatttgg
      481 ggatgggagg aatcatcggg tcattcatct ccgcgatttt ccggctgtgg atttctatct
      541 tgccggaggg ggtgcggaac ggatggtgga gggggtcttc aatttcttcc tgaaaagcca
      601 cccaaggttt ccggacctcg taagaatgga ccttttcccc cttgaaccgg gggtaatcgg
      661 gaagccccgg ggtatcggag agaaattcct gaagccactc ctcgttggtc ttgtcgttgt
      721 agcccgcaag attcaacagc gaagccagct cggagaagat atccaggtcc gatttcaccc
      781 cggggagagg ttccacggcc ttctccatca ggatattgta agggcccccg gtccagggct
      841 ggcccacatc ctgccgctcg aggaaatggg ccacagggag gactatatcg gcatgacggg
      901 cagtgggagt catgaagagc tcgtggacca ccaccagttc cggtttttgt agagccttaa
      961 cccctttgtt ggtattcagc cattggttca gcaggttgca gcccaaaatg tagaggacct
     1021 tgatgtcgct gggaaacctc ccttccttcc cccggagaag gaggtcatac aattcggaaa
     1081 catgaaccat gggattctgg ctttcaggga cgggtaggcc cttgcgcaaa atccccaggg
     1141 ccattcggtc cgttcctccg gagacatggc ctcctacaac gccgatgttc cctgtcatgg
     1201 ccgccagggt tgaagcggcg cggtggtatt gctctccgta agcagtccgc cccggggccc
     1261 acccggcata cagggccgcg ggtttgagca gggcgtagtc gcgggccagc cggacaatga
     1321 cctccgaggg gactccggta atttcctccg cccaggcagg attcttggga attccatcct
     1381 cctcgcccat gacgtattcc ctgaatttat caaacccgga agtgtaagtt tcgatgaacc
     1441 ggcgatcgta aaggtcttcg gtgatcataa cctgggccat ggccaccaga agccctgtgt
     1501 ccgtggccgg cctgatggca atccactgat cggccaggct cttgcccgtg gggctcaacc
     1561 gggggtctac gcagatgatc cgggccccct tctttttggc ctcggaaagg tagtaggccg
     1621 tatccggccc gaaccgagtc tccagggggt tccaacccca gagaaggatc agtttcgaat
     1681 ggagaaggtt gtcccggctg ttccgggtga agaaagtccc gaaggtcatc agggaggaga
     1741 agatggccgc ctctgcggag gtgttccccc accaggtggt gcacccgccg aagagggaga
     1801 agaagcgccg gccggtgcgg ccggtgttgt gcagcgggct catggagccg aaatagtcca
     1861 tcaggaaaac ggattgaggg ccgaactgat ctttcactct cgcaagttcc cgggcaacct
     1921 ggtccagggc ctcctcccag gagatgggct cgaattcccc gcttcccctc tcccccgccc
     1981 tcctcagggg cactttcagc cggtcccccg catataagac atcgcgctgg gcaaggcctc
     2041 gcgcgcaggc cttgagcccg ggacccggtt gatcatcggt ggtgattttt tggacccgcc
     2101 cctcggaaac atgaaccttg agaagacagc gcgacccgca atcaaaggaa cacgtggagg
     2161 tgacgacccg atcatcccgg tttgattccc cgttggtgct cattcttcaa atcctctgga
     2221 catccggaga aggcctcggg acctcatcaa cctgaacgcc cggaccacaa gcaacagcag
     2281 gatagcggga acccaaaccc atcggacctc gctcaggatc acttccgttc cccaagaact
     2341 gaagaaagat tttaggccga tgggtgagat ttttatgggg gcccagggaa aggaatagcg
     2401 gtccgtatcg aagggcgaga aaaaggcgac cccatagccc cttccggtta aggcatcc
//
LOCUS       DASPRR010000091         6326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2282034, whole genome shotgun sequence.
ACCESSION   DASPRR010000091 DASPRR010000000
VERSION     DASPRR010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282034"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /gene="rpsJ"
                     /locus_tag="VEL68_03175"
     CDS             <1..242
                     /gene="rpsJ"
                     /locus_tag="VEL68_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXZ35000.1"
                     /translation="EIVDTAKRTGARVAGPIPLPTSINKYCVLRSPHVDKKSREQFEI
                     RTHKRLLDILEPTQQTIDALMKLDLAAGVDVEIKL"
     gene            347..994
                     /gene="rplC"
                     /locus_tag="VEL68_03180"
     CDS             347..994
                     /gene="rplC"
                     /locus_tag="VEL68_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXZ35001.1"
                     /translation="MLKGIIGKKLGMTQIFHEEGGVVPVTVIQAGPCKVVQVKTAEKE
                     KYGAVQLGFEERAAQKVKKPLQGHFTKAQVPAFRYLREFRVSDPGAFQVGQEITVESF
                     QVGDRVDIIGISKGKGFMGVVKRWHFRGGRATHGSMFHRAPGSIGASSYPSRVWPGQK
                     MGGHMGDRKATLQNLEVIDIRPRQNLLLVKGAVPGGSEGLVLVRQAKKKKSKAGS"
     gene            1015..1638
                     /gene="rplD"
                     /locus_tag="VEL68_03185"
     CDS             1015..1638
                     /gene="rplD"
                     /locus_tag="VEL68_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXZ35002.1"
                     /translation="MAKVEVIDIQRQKVAEMEVDDRLLQATEKSHLIYDVVRWQLACR
                     RSGTAATKERSAVSGGGKKPWKQKGTGRARAGHSRSPLWRGGGTIFGPQPRDYSYHLP
                     KKVREAGLIAALSSKYREEKLVVLDRFGLEAVRTRRFREVLKGLGVSSALIVIDGPDE
                     ILEKSARNIPGVQVLRVPGLNVHDILRHEYLIFLRSSLEKVERNLQS"
     gene            1635..1925
                     /gene="rplW"
                     /locus_tag="VEL68_03190"
     CDS             1635..1925
                     /gene="rplW"
                     /locus_tag="VEL68_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXZ35003.1"
                     /translation="MKEPQQIIKRPLITEKSTRQKEKRNQIAFVVDPRANKIQIRQAV
                     EKLFKVKVTAVHTLNLAGKRKRMGRFFGWQSDWKKAIVTLKEGERIEFFEGA"
     gene            1947..2768
                     /gene="rplB"
                     /locus_tag="VEL68_03195"
     CDS             1947..2768
                     /gene="rplB"
                     /locus_tag="VEL68_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXZ35004.1"
                     /translation="MGLKTYKPTSAGRRFQTTLDFSELTRKKPERSLLVPRKKTGGRN
                     VYGRITSRYIGGGHKRMYRIIDFKRDKRDIPARVASLEYDPNRSANIALLNYSDGEKR
                     YILAPLDLKVGDTVMSGERADIMPGNALPLKNIPLGTMVHNLEMKPGKGGQLIRSAGS
                     AGQLMAKEGNYATLKMPSGEVRLIHVNCFATVGQVGNLDQENVTIGKAGRKRWLGRNP
                     RVRGVAMNPVDHPLGGGEGKSSGGRHPVTPWGVPTKGYKTRQNKATDKFIVKRRR"
     gene            2865..3170
                     /gene="rpsS"
                     /locus_tag="VEL68_03200"
     CDS             2865..3170
                     /gene="rpsS"
                     /locus_tag="VEL68_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXZ35005.1"
                     /translation="MARSIRKGPYVEPNLLKKVQEVADTKSRKVIKTWSRRSTILPDM
                     VGLTFAVHNGKKFIPVFVTENMVGHKLGEFSPTRTFFSHSGDRKTKVKAAAAPKPAA"
     gene            3181..3513
                     /gene="rplV"
                     /locus_tag="VEL68_03205"
     CDS             3181..3513
                     /gene="rplV"
                     /locus_tag="VEL68_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXZ35006.1"
                     /translation="MEARAIAKYLRISPRKTRLCADLIRGKRAEEAVNILSHTPKAGA
                     RMISKVVQSALANARQDKSIDVDTLYVKTVFVNQGPTLKRWQPKPMGRAGRIRKRTCH
                     VTVVLSET"
     gene            3553..4209
                     /gene="rpsC"
                     /locus_tag="VEL68_03210"
     CDS             3553..4209
                     /gene="rpsC"
                     /locus_tag="VEL68_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXZ35007.1"
                     /translation="MGQKVNPIGLRLGIIRTWDSRWYAEKFYSRFLQEDILLRKFIKK
                     KVYHAGASKIEIDRTANRAKITIRAARPGIIIGQKGAEIDKLQKELQKLTQREISINI
                     QEVRRVETDAQLVAENVALQLERRVAFRRAMKKGVTSGLKFGAQGIKISRAGRLGGAE
                     IARSEWYREGRVPLHTLRADIDYGFAEARTTYGQIGIKVWIYRGEILPAVRGRREMAP
                     "
     gene            4225..4656
                     /gene="rplP"
                     /locus_tag="VEL68_03215"
     CDS             4225..4656
                     /gene="rplP"
                     /locus_tag="VEL68_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXZ35008.1"
                     /translation="MLSPKKVKFRKQQKGRRRGTASRGNSLNFGDFGLQAAACGWLTT
                     RQIEAARITMTRHIKRGGKIWIRIFPDKPICKKPAETRMGKGKGAPEDWVAVIQPGRV
                     LYEMEGVSRDTALEAFRLAAHKLPLKTKFIAREGRVESAGT"
     gene            4637..4843
                     /gene="rpmC"
                     /locus_tag="VEL68_03220"
     CDS             4637..4843
                     /gene="rpmC"
                     /locus_tag="VEL68_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXZ35009.1"
                     /translation="MKARELKEMSEAELRQKEKEVTEQLFNLKFQHATGQLENTARLP
                     QVRKDLARVKTVLREKVLAAAKAK"
     gene            4854..5111
                     /gene="rpsQ"
                     /locus_tag="VEL68_03225"
     CDS             4854..5111
                     /gene="rpsQ"
                     /locus_tag="VEL68_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXZ35010.1"
                     /translation="MKARGIRKTMVGRVVSDRMEKTRVVVVQRLVKHPLYQKYIRRRS
                     RFKVHDETNASRAGDRVLIIETRPLSREKRWRVKEVLERAQ"
     gene            5170..5538
                     /gene="rplN"
                     /locus_tag="VEL68_03230"
     CDS             5170..5538
                     /gene="rplN"
                     /locus_tag="VEL68_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXZ35011.1"
                     /translation="MIQMQTSLEVADNSGAKRLSCIKVLGGTKRKYASLGDVIIVSVK
                     EAIPHSKVKKGDVMRAVIVRTVKEVRRLDGSYIKFDDNSAVLINPQNEPVGTRIFGPV
                     ARELRAKKFMKIISLAPEVL"
     gene            5567..5899
                     /gene="rplX"
                     /locus_tag="VEL68_03235"
     CDS             5567..5899
                     /gene="rplX"
                     /locus_tag="VEL68_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXZ35012.1"
                     /translation="MKYVSFHVRKNDLVEVLSGKEKGKTGKVLRVFPKNDRVLVEKIN
                     FVKRHSRPTGRTRQGGIIQKEAPIHVSKVLLVCPKCNKGERMRSKTLEDGKKALVCKA
                     CGEQMERK"
     gene            5917..>6326
                     /gene="rplE"
                     /locus_tag="VEL68_03240"
     CDS             5917..>6326
                     /gene="rplE"
                     /locus_tag="VEL68_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXZ35013.1"
                     /translation="MTRAKEVYLKEVVPQLMKSFGYKNRMQVPQLEKITLNMGLGEAV
                     QNVKILDSAVEELSLIAGQKAVITRARRSIAAFKLREGMPIGVTVTLRRNRMMEFFDK
                     LVNVALPRVRDFRGVSGKAFDGRGNYSLGIREQI"
BASE COUNT         1583 a         1620 c         2008 g         1115 t
ORIGIN      
        1 aggagatcgt ggacacggcc aaacggacgg gagctcgggt cgccggaccg atccccctgc
       61 ccacgtcgat taataagtat tgtgttcttc ggtctcccca tgtggacaag aaatccaggg
      121 agcagttcga aatccggacc cacaagcggc tcctggacat tctcgagccc acccagcaga
      181 ccatcgatgc cttgatgaag ctcgacctgg cggcaggggt ggacgtggaa ataaagctgt
      241 aaagaagcaa tcagcgttca gcggtcagca aagacacttc agaaaaaagt ttttttgctg
      301 aacgctgagc gctgaaggct gatcgctatt ttgaagggtt tccatcatgt taaaagggat
      361 catcggtaaa aaattgggca tgacccaaat tttccacgaa gagggcgggg tggttccggt
      421 caccgtcatc caggccggtc cctgcaaagt ggtccaggtc aagacggcag aaaaggaaaa
      481 atacggcgcg gtccagttgg ggtttgaaga gagggctgcc caaaaggtga aaaagcccct
      541 ccagggccat ttcaccaagg ctcaggttcc ggcgttccgg tatttgaggg agtttcgggt
      601 atccgatccc ggcgctttcc aggtgggaca agagattacg gtggaatcct ttcaggtggg
      661 agaccgggtg gatattatcg gaatctccaa gggcaagggt ttcatggggg tggtcaaacg
      721 ttggcatttc cggggggggc gggccaccca tgggtccatg tttcaccgcg ctcccggctc
      781 catcggagcc agctcctatc cttcccgcgt ctggcccggg cagaagatgg gagggcatat
      841 gggagaccgg aaagccaccc tgcagaatct ggaagtgatc gatatccgcc cgaggcaaaa
      901 tttgcttctg gtgaaggggg ccgttcccgg agggtccgag gggctggttc tggttcgcca
      961 ggcgaaaaag aaaaaatcca aggcaggaag ttgaggcccg acaagggagc ggggatggcc
     1021 aaggtagagg taatcgatat tcagcggcaa aaagtggcgg agatggaggt ggatgaccgt
     1081 ctcctgcagg cgacggagaa gtcccacttg atctacgacg tggtgcgttg gcagctggcc
     1141 tgccgccgga gcggaacggc cgccaccaaa gagcggtcgg cggtcagcgg cggaggcaag
     1201 aagccctgga agcaaaaggg aaccggaagg gcccgggcgg gccattcccg gtcccccctg
     1261 tggcggggtg ggggaacgat ctttgggccc caaccgaggg actattccta ccatctgccc
     1321 aaaaaagtcc gtgaggccgg tctgatcgcc gctctctctt ccaaataccg ggaggagaag
     1381 ctggtggtgt tggaccggtt cgggctggaa gcggttcgga cccggcgttt tcgggaggtc
     1441 ctgaagggtc tgggcgtctc cagcgctctc atcgtcatcg acgggccgga cgaaatcctg
     1501 gagaaatcgg cacgcaacat tcccggggtt caggtgcttc gggttccggg cttgaacgtc
     1561 cacgacatcc tgcgccatga atatctgatt tttctgcgct ccagcctgga gaaggtggaa
     1621 aggaatctgc aatcatgaaa gaaccacagc agatcatcaa acggcccctg atcaccgaaa
     1681 agagcacccg ccaaaaagag aagagaaacc aaattgcttt tgtggtcgat ccccgggcca
     1741 ataaaatcca gattcgccag gcggtggaga aactgttcaa agtgaaagtc acggcggttc
     1801 ataccctgaa cctggcgggg aaacggaagc ggatggggcg gttcttcggc tggcaatcgg
     1861 attggaaaaa ggccatcgtg accctgaagg aaggggagcg gatcgagttc ttcgaaggcg
     1921 cctaatcgga aacgggaagg gtacccatgg gactgaaaac atacaaaccc acctcggcag
     1981 gcagaaggtt tcagaccacc ctggattttt ccgagctgac tcggaagaag ccggaaagga
     2041 gtctgctggt tccccggaag aaaaccggag gccgaaacgt atacggacgg atcaccagtc
     2101 ggtacatcgg ggggggtcat aagcggatgt accggatcat tgatttcaag cgggacaagc
     2161 gagacatccc cgcccgggtg gcctccctgg agtatgatcc caatcggtcg gccaacatcg
     2221 ccctgcttaa ctatagcgac ggagagaaac gatacatttt ggcccccctg gacctcaaag
     2281 tgggagatac ggtcatgagc ggggaacggg cggacatcat gcccggaaac gctctgcccc
     2341 tgaagaatat ccccctgggc accatggttc ataacctgga gatgaaaccc gggaaagggg
     2401 gccaactcat tcgcagcgcg ggctcggccg ggcagctgat ggccaaagaa gggaattatg
     2461 ccaccctcaa aatgccctcg ggcgaagtcc gtctcatcca cgtgaactgc ttcgccaccg
     2521 tgggacaggt ggggaatctg gatcaggaaa acgtcactat cggaaaggcc ggacggaaac
     2581 gctggttggg acggaacccc cgggtccggg gcgtggccat gaacccggtc gaccatcccc
     2641 tggggggagg agaagggaaa tcatcgggag gccggcatcc ggtcaccccc tggggcgttc
     2701 ccaccaaggg atacaagact cgtcagaaca aggcgacgga taaatttatc gttaaacgga
     2761 gaagataagt aatgagttcg gaagcaatgt agctcggaaa gaaacggaaa ggatctttta
     2821 ctccgaactc gaaactccga actccgaact gtgaggtgat actcgtggcc agatcgattc
     2881 gcaagggtcc ttacgtggag cccaatttgc tgaagaaggt tcaagaggtg gcggatacca
     2941 aaagccgaaa ggtgatcaag acctggtccc gccggtcgac gattttaccg gacatggtgg
     3001 gcctcacctt tgccgttcac aacgggaaaa aattcattcc cgtttttgtg accgaaaaca
     3061 tggtcggcca caagctggga gaattttcgc ccacccgtac cttcttcagc cactccgggg
     3121 accgcaagac caaggtgaag gccgcggccg cccccaagcc ggcggcatag gggaggcagg
     3181 atggaagccc gagccattgc caaatatctt cgaatctccc cccgcaagac gcgcctttgc
     3241 gccgacctca tccggggaaa gcgggcagag gaggcggtga acatcctcag ccatactccc
     3301 aaggctgggg ccagaatgat ctccaaggtt gtacagtcag cgctggccaa tgcgcggcag
     3361 gataaatcca tcgatgtgga caccctttat gttaagaccg tattcgtgaa tcaggggccc
     3421 actttgaaac gttggcagcc caagcccatg ggacgagcgg gtcgcatccg caaacgaacc
     3481 tgccatgtga ccgtggtgtt gagcgaaacc taaaaactca aaatcgttgc gaggaaggga
     3541 aggaggtcat gcttgggtca gaaagtcaat ccgatcggac tccgactggg gatcattaga
     3601 acctgggatt ctcggtggta tgcggaaaag ttctattccc ggtttttgca ggaggacatc
     3661 ctcctgcgca agttcatcaa gaagaaagtc taccatgcgg gagcctccaa aatcgagatc
     3721 gatcggaccg ccaaccgggc caaaatcacc attcgggcgg cccgtcccgg gatcattatc
     3781 gggcaaaaag gagcggagat cgataaactg cagaaagagc tgcagaaatt gacccagcgg
     3841 gagatctcca tcaacatcca ggaagtccgg cgggtggaga ccgacgccca gctggtggcg
     3901 gaaaatgtgg ccctccagct ggaacggcgg gtggctttcc ggcgggccat gaagaaaggg
     3961 gtcacctccg gtttgaaatt cggcgcccag ggaatcaaaa tttcccgcgc cggaaggctg
     4021 gggggggcgg agattgcccg ttccgagtgg taccgggaag gacgggttcc tctccatacc
     4081 ctgcgtgcgg atatcgatta cgggtttgcc gaagcccgca ccacctacgg acagattgga
     4141 atcaaggttt ggatttaccg gggcgagatt cttccggcgg ttcgcggacg gagggagatg
     4201 gccccctaga gagccggaga ccctatgtta tcacccaaaa aagttaagtt tcgcaaacag
     4261 cagaaaggca gacggagagg gaccgcgtcc cggggaaaca gcctgaactt tggagatttc
     4321 ggcctgcagg cggcggcctg cggatggctg accacccggc agattgaagc ggcccggatc
     4381 accatgaccc ggcacatcaa acggggcggc aagatctgga tccggatttt cccggacaaa
     4441 ccgatctgca aaaaaccggc cgaaacccgg atgggaaagg gaaagggcgc cccggaagat
     4501 tgggtggcgg tgatccagcc cggccgggtg ctgtacgaaa tggaaggggt gagccgggat
     4561 acggccctgg aggcttttcg tctagccgct cacaagcttc ccctgaagac caaatttatc
     4621 gcacgggagg gcagggttga aagcgcggga acttaaggag atgagcgaag cggagcttcg
     4681 ccagaaggaa aaagaggtta ccgaacaatt gttcaacctg aaattccagc atgctaccgg
     4741 gcagctggag aacaccgcgc ggttgcccca ggtgcgcaag gacctggccc gggtgaagac
     4801 cgtcctgcgc gagaaggttc tcgcggcggc caaggccaaa tgagggaatg ggaatgaagg
     4861 ccagagggat ccgaaagacg atggtgggta gggtggtcag cgaccgcatg gaaaaaacga
     4921 gagtcgtggt ggtccagcgc ctggtcaaac accccctgta ccagaaatat atccggagac
     4981 ggtcacgttt caaagtccat gatgagacca atgccagccg ggcgggagat cgggtgctca
     5041 tcatcgaaac ccggccactc agccgggaga aacggtggcg ggtgaaagag gttctggagc
     5101 gggcccagtg accctccgga gagtctgctc cacaggggcg gacggggaaa agaataggcg
     5161 ggtatagcca tgattcaaat gcagacctca ctagaagttg ccgacaattc aggggccaag
     5221 aggctttcct gcataaaggt cctgggaggg accaagcgga agtatgccag cctgggggac
     5281 gtgattatcg tttcggtcaa ggaagccatt ccccattcca aagtcaagaa gggcgacgtc
     5341 atgcgggcgg tcatcgtccg gacggtcaaa gaagtccgtc ggcttgacgg aagctacatc
     5401 aagttcgatg acaactcggc ggttctaatc aacccgcaga acgaaccggt ggggacccgg
     5461 atcttcggtc ccgtggcccg ggagctgcgg gccaagaaat tcatgaaaat catttccctg
     5521 gccccggaag tcctgtaaga tcgagcccag gagagtgagg ccatcgatga aatacgtaag
     5581 tttccacgta cggaaaaacg atctggttga ggtcctgagc ggaaaagaaa agggcaaaac
     5641 cgggaaagtc ctccgggttt tccccaaaaa tgatcgggtg ctggtggaaa agatcaattt
     5701 cgtgaaacgc cattcccggc ccacgggcag aacccggcag ggggggatca tccagaaaga
     5761 ggctcccatc catgtctcca aggttctgct ggtctgtccc aaatgcaaca aaggagagcg
     5821 gatgaggagc aaaaccctgg aggacgggaa aaaggctctg gtttgcaaag cttgcggcga
     5881 acagatggaa aggaagtaaa ggaaagggcg tcggccatga ctcgggcgaa agaggtctat
     5941 ctcaaagaag tggttcctca gctgatgaaa tccttcggct ataagaaccg gatgcaggta
     6001 ccccagctgg agaagattac cctgaacatg ggcttggggg aggcggttca aaacgtaaag
     6061 attctcgatt ccgcggtgga ggaactgagt ctgatcgccg ggcaaaaagc ggtgatcacc
     6121 agggccagac gctcgatcgc cgcctttaag ctgcgggagg gaatgcccat cggagtgacg
     6181 gtcaccctcc gccgaaaccg gatgatggag ttttttgata agctggtgaa cgtggccttg
     6241 ccccgggtca gagatttccg cggggtgtcg gggaaggctt tcgacgggcg gggaaattat
     6301 tccctgggaa ttcgggagca gattat
//
LOCUS       DASPRR010000092         4253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1959015, whole genome shotgun sequence.
ACCESSION   DASPRR010000092 DASPRR010000000
VERSION     DASPRR010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959015"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..139
                     /locus_tag="VEL68_03245"
     CDS             5..139
                     /locus_tag="VEL68_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ35014.1"
                     /translation="MLKRTPLRRLGDVQEIASLVSFLASPEADFFTGQIFSPNGGLVI
                     "
     gene            266..556
                     /locus_tag="VEL68_03250"
     CDS             266..556
                     /locus_tag="VEL68_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXZ35015.1"
                     /translation="MNKQPAVYILASKKNGTLYVGVASDLAKRVWEHRNNMVEGFTKK
                     YNVHQLVWYELHDSIESAIVREKRLKDWKRAWKFKLIESKNPEWVDFFDAIF"
     gene            657..1481
                     /locus_tag="VEL68_03255"
     CDS             657..1481
                     /locus_tag="VEL68_03255"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ35016.1"
                     /translation="MSKGYSRLLVDARGDICWVTVNRPEDRNSIDTALMKELERVLKE
                     AEKTAARAIVFTGSGESHFIGGADGVEMMQCSPKGAREFSTRIQKLFNRMEASPLILV
                     AAINGLCFGGGFEFAMACDFRVASEEARIGLPEVKVGIIPGGGGTQRLPRLVGMGRAM
                     EMILSGKLFKGREALEAGLVHSVAASDQLLKEAERVLEPVLRNPQHALSQAKKAVRAS
                     QNTTLAKGQKIESLVFSRCFSQGYFLELMRKQLKEGTLKTTVKLPKWVLEERKKKP"
     gene            1478..2053
                     /locus_tag="VEL68_03260"
     CDS             1478..2053
                     /locus_tag="VEL68_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ35017.1"
                     /translation="MKVVGIAGSLRPQSNTLYYVKTALGVLEGQGIQTELVTLQGKEI
                     RPCTGCYDCTKQGRCTIEGDDFDEILEKMRMAEGLILGSPVYLSSVVPQMMALLSRAT
                     FVAYWNNKFLSGKVGGPITVARRAGHNMAFSQLLLWFFINGLIVPGSTYWNVGMAGTG
                     GARDAEKDAEGIQTVTNFAQNMTNLMKKLFL"
     gene            2217..3734
                     /locus_tag="VEL68_03265"
     CDS             2217..3734
                     /locus_tag="VEL68_03265"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ35018.1"
                     /translation="MTGIGFWLTKRERLHGDREAVVDGQRRLSYRALNGRVNRLARSL
                     QTLGLKQGDRISVLAYNGLEYVETIFAAAKLGLILVPLNWRLAPAELAFILSDSTTET
                     LLFDSEFAKTAETLRGQTPLRRFVAFGEKEIPGATAYEKALSSEEDGEPTPDGPVDLD
                     RPHIIMYTAGTTGRPKGAVLSQGASFWNAVNMSLCVDLTSHDRTLTVLPMFHIGGIGL
                     YTLPTLFQGGTVVIQRTFDPKVTVRLIKEERIDVFFGVPAIFLFLIQDPAFKSGGMAR
                     LRSVMSGGAPLPVSLVHQYKEAGIILQQGFGMSEAAPGIAALDRDRAVAKAGSIGRAL
                     FSMEVRVVDSQNRDLPPNREGELLIRGPNVMQKYWNRPEATAEAFTDGWLHTGDLARM
                     DEEGDLYIVDRKKDMFISGGENIYPAEVENVIYELSQVGEAAVIGVKDPKWGEVGRAI
                     VVLKAGQKLTEAEILDYLKGKLAKFKVPRSVVFVDQLPRNAAGKVLKNILREKHP"
     gene            3933..>4253
                     /locus_tag="VEL68_03270"
     CDS             3933..>4253
                     /locus_tag="VEL68_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35019.1"
                     /translation="MKYPKFLLWCVALIVPIFILAPGGFAQGPIKLGAIFPLADITGD
                     QGAKAMKLAVKEINQAGGLLGRQVELIIVDDEMKAEKGAAAVEKLVTVDKVDILVGGM
                     ASGVH"
BASE COUNT          989 a         1099 c         1293 g          872 t
ORIGIN      
        1 ttacattctc aagaggactc cactccgacg tctgggcgat gtacaggaga tcgccagtct
       61 ggtctctttc ctggcgtccc ccgaagcgga tttctttacc ggacagattt tcagcccgaa
      121 cggcgggctg gtgatttgac ctgctagcct tactcttccc cgcgggggag gataagtagg
      181 aagcttaagg acctagaatt cttataattg gcataagtct tgattatttg tcattccggc
      241 gaaagccgga atccagagaa aggcattgaa taagcagccc gccgtataca tcctggcgag
      301 taagaaaaat ggcacgctct acgtaggagt cgcttctgat ttggccaaga gggtatggga
      361 gcatagaaat aatatggtgg aagggtttac caaaaaatac aacgtccatc agttggtttg
      421 gtacgaactt cacgacagta ttgaatccgc aatcgttcga gagaagagat taaaggattg
      481 gaagcgcgct tggaaattca aattaatcga aagtaaaaat ccggagtggg ttgatttctt
      541 tgatgcaatt ttttgactgg attccggctt tcgccggaat gacgaaatac ataggatggc
      601 tcttatgccc acaagtgggc taggctggaa gagagtgaaa tggcaggaga aaggaaatgt
      661 ctaaaggata ctcacggctt ctagtcgatg cccggggaga catatgctgg gtcactgtca
      721 accgtcccga ggaccggaac tcgatcgata cggccctcat gaaggaactg gagcgggtgc
      781 tgaaggaagc agaaaagacg gcggccagag cgatcgtatt taccggctcg ggggagagcc
      841 atttcattgg cggggccgat ggcgtcgaga tgatgcagtg cagccccaaa ggggcccgcg
      901 agttttcaac ccgaatccaa aagctgttca accgcatgga agcctccccg ctcatcctgg
      961 tggcggccat caacggcttg tgttttgggg gcggttttga attcgccatg gcctgcgact
     1021 tccgggtggc cagcgaggag gcgcgaatcg ggctcccaga ggtaaaagtt gggatcattc
     1081 ctgggggcgg tggaacccaa aggcttcccc gcttggtggg aatggggcgg gccatggaga
     1141 tgatcctcag cgggaagctt ttcaagggca gggaagcttt ggaagcgggg ctggtccatt
     1201 cggtggctgc ctcggaccag ctcttgaagg aggccgagag ggtcctggaa cccgtcttgc
     1261 gcaatcccca gcacgccctt tctcaggcca agaaggcggt tcgggcctca caaaacacga
     1321 ccctggctaa aggccagaag atcgaaagct tggtcttcag ccggtgtttc tcccagggct
     1381 attttttgga gctgatgaga aaacagctga aggaaggcac cctgaagacc acggtcaagc
     1441 tccccaaatg ggtacttgaa gaaaggaaga agaagccatg aaagtggtgg gaatcgccgg
     1501 gagcctgagg ccccagagca atacccttta ctacgtgaag accgccttag gggtgctcga
     1561 agggcagggg attcagaccg agctcgtcac cctccagggg aaggagatca ggccctgcac
     1621 gggctgctac gattgcacca agcagggccg ctgcaccatc gagggggatg acttcgatga
     1681 gatcctggag aagatgcgca tggctgaagg gctgatcctg ggatctccgg tctacttgag
     1741 ttcggtggtc ccccagatga tggctctcct gagccgggca actttcgtgg cctattggaa
     1801 caacaagttt ttgagcggca aagtgggagg gccgatcacc gtggcccgga gggcgggcca
     1861 caacatggcc ttttcccagc tccttctatg gttcttcatc aatggtctca tcgtgcctgg
     1921 ttcgacttac tggaacgtgg gcatggctgg aacgggcggc gcccgggatg ccgagaagga
     1981 tgcggaggga atccagaccg tgaccaactt tgcccagaac atgactaatt tgatgaaaaa
     2041 gcttttcctt taaatatgaa gattcttggc aaagtcaatt catccgtcgt tccggcgaaa
     2101 gccggaatcc agaattgcct gaaaatcctg gattccgggt cgcgcttcgc ttgcccggaa
     2161 tgacaatttc cccttgcttt caacagtttt gcaggagcct ctaaggagag gaagaaatga
     2221 cggggatcgg attctggctg acaaaaagag agcggctcca cggcgaccgc gaggcagtgg
     2281 tggacggaca gcggcggctg agttaccggg ccctcaacgg tcgggtcaat cggctggccc
     2341 gttccctcca gaccttgggg cttaagcagg gggaccggat ttcagtcctg gcgtacaatg
     2401 gccttgagta cgtagagacg atctttgcgg ccgccaagct gggtttgatt ctcgtacccc
     2461 tgaactggcg cctggcaccg gcggaactgg cgttcatcct ttcggacagc accaccgaaa
     2521 ccctcctttt cgactctgag ttcgccaaaa cggccgaaac ccttcgaggc cagaccccgc
     2581 tccggcgctt cgttgccttc ggtgagaaag aaatcccagg ggccaccgcc tacgagaagg
     2641 ccctgtcttc agaggaggac ggcgaaccca cacccgatgg acccgtggac ctggataggc
     2701 cccatatcat tatgtatacc gccggcacca cgggaagacc caagggggct gttttgagcc
     2761 agggggccag tttctggaat gcggtcaaca tgtctttgtg tgtggacctg acctcccacg
     2821 accggaccct gaccgttctg cccatgtttc acatcggggg aatcggcctc tacactctcc
     2881 caacgctttt ccagggagga acggtggtca tccagagaac ctttgatccc aaggtgaccg
     2941 tccgcctcat taaagaagag aggatcgatg ttttcttcgg cgtgccggcc atatttctct
     3001 ttctaatcca ggacccggcc tttaagtccg gcgggatggc ccgtctccgg tcggtcatga
     3061 gcggaggagc gcccctgccg gtgagcctgg ttcatcaata taaggaggcc ggcatcatcc
     3121 tgcagcaggg tttcggcatg agcgaagccg ctccggggat tgcggcccta gaccgggacc
     3181 gagccgtggc caaggcgggc tccatcggcc gcgccctctt ctccatggaa gtccgcgtgg
     3241 tggattccca aaaccgggac cttcccccga accgggaagg ggaactcctg atccgcggcc
     3301 ccaacgtcat gcagaaatac tggaaccggc ccgaggccac ggccgaagcc ttcaccgacg
     3361 gatggctcca caccggagac ctcgctcgga tggacgagga aggagacctc tatatcgtgg
     3421 accgaaagaa ggacatgttc atttccggag gggagaatat ctatccggcg gaggtggaaa
     3481 atgtcatcta cgagttatcc caggtcggag aagcggcggt catcggagtc aaggacccaa
     3541 aatgggggga agtcggccgt gccattgtag tcctgaaagc ggggcagaaa ctgacagaag
     3601 ccgagattct cgattatctg aaggggaagc tggccaagtt taaggtcccc cgttccgtgg
     3661 tattcgtgga ccagctgccc cgaaatgctg ctggcaaggt gcttaaaaat attctacggg
     3721 aaaagcaccc ctgaaagaat cctctttacc cggtaaatgg atccagatag aatccaagtc
     3781 gccagccaag gatcctcttg gttttttagg ggatagaaat cgaagtgcct ctagaaaggg
     3841 atcatctcgc cccgtaattt gcggggatgg attataaacg gcagaaggaa tcgacaagga
     3901 atctttagaa atctcgaaag aggaggtgcc aaatgaagta ccccaagttc ctgttgtggt
     3961 gcgtagccct tatcgttccc atctttatcc tggcgccggg aggatttgcc cagggaccca
     4021 tcaagctggg agccattttc cccctggctg acattaccgg agatcagggg gccaaggcca
     4081 tgaaactggc cgtgaaggag atcaatcaag cgggcgggct gctgggccgc caggtggagt
     4141 tgatcatcgt cgatgacgag atgaaggcgg aaaagggcgc ggccgccgtg gagaagctgg
     4201 tcaccgtgga caaggtggat atcctggtgg gcggcatggc cagcggcgtt cac
//
LOCUS       DASPRR010000093         6176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1798137, whole genome shotgun sequence.
ACCESSION   DASPRR010000093 DASPRR010000000
VERSION     DASPRR010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798137"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /gene="gspF"
                     /locus_tag="VEL68_03275"
     CDS             <1..1223
                     /gene="gspF"
                     /locus_tag="VEL68_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HXZ35020.1"
                     /translation="DSLPVFEYEGLSNSGKALRGIIDAESLRNARSKLRSQGVYPTEI
                     REEAAAVALPGTSFRLFSGVPPKELARVFRQLATLVGAGIPLVSSISALIDQTGNTIL
                     RKVLTRIRESVREGGSLADALAAHPQVFPPILVGLVRAGEVSGALALTLGRWADFSEH
                     QVALRQRVRAALTYPVFMFFIGAGVLFFLMTFVVPTVTRVFSDLGQSLPWPTLILMAV
                     SDFMSRFWWALVGAFILFVLWARKFLRSERGGLFWDRLKMKVPLARELHRKLAVSRWS
                     RTLGTLLSGGLPLLQALEYSQGVAGNRLFSQAIGQARDRVREGEEMALSLKGSGLFPS
                     VLLEMVAVGEKSGELSPMLEKVASSLENEAESDLRGLIALLEPLMILIMGVGVGFIAL
                     SVLLPILEMSQMVR"
     gene            1338..1766
                     /gene="gspG"
                     /locus_tag="VEL68_03280"
     CDS             1338..1766
                     /gene="gspG"
                     /locus_tag="VEL68_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXZ35021.1"
                     /translation="MENRKAGGFTLIELMVVIVILGILAGLVLPRFMGRTEEAKKTKA
                     RLQIENLEAALKLYKLDNGTYPTTEQGLESLVLKPSTGIIPKNWREGGYLEKSKVPED
                     PWGRPYVYISPGIKNRDFDLKSLGADGEEGGEGENADIER"
     gene            1732..2265
                     /locus_tag="VEL68_03285"
     CDS             1732..2265
                     /locus_tag="VEL68_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35022.1"
                     /translation="MEKGRTRTSKGSPGFTLAELVVVLFLLGFIFLLTFPNFRELLEP
                     RDIKRATVRLAGSLRYAQSQAAATKQRFRLNIDIKENAYWVSREGERDTFLRDSSPMG
                     KPDYLPAGVVFLDVTHPERGKLREGTAHIDFSPTGWADECEIHLGKGEEEVFTIFIHP
                     LGGKMEVSAGYVERKTS"
     gene            2267..2623
                     /gene="gspI"
                     /locus_tag="VEL68_03290"
     CDS             2267..2623
                     /gene="gspI"
                     /locus_tag="VEL68_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HXZ35023.1"
                     /translation="MKKGFTLLEVMIALALLALVGVAFLRAQAGSVRLLDESSQISLA
                     TLLAREKMAELESVGFPELGKSSGTGGEAYPLFSWEQVVTSTDLPALRQALVRVLWKE
                     GTRERTLELVAYFAQK"
     gene            2796..3479
                     /locus_tag="VEL68_03295"
     CDS             2796..3479
                     /locus_tag="VEL68_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXZ35024.1"
                     /translation="MRNAQSEDPELFPRGSGFTLFEVLMTLSILGVVLSLLYLTFHQS
                     MAVMAETDDRAEVIQQGRMILERIAGDLKGAVIIAQKEGFQAYRTGLVGRSTSEGKDF
                     LDRMDFTTGLPSFSSRPEGGWGFFEVGYFLDRSPGGKGLTLFRRQDDGMDGNLLQGGR
                     SVAICDRVRSLGFSYYDRQGGRGKEWNSLEGERRNLLPSRVEIQLKLEDSRGRVHEFR
                     TQTYLPMSE"
     gene            3954..4907
                     /gene="gspK"
                     /locus_tag="VEL68_03300"
     CDS             3954..4907
                     /gene="gspK"
                     /locus_tag="VEL68_03300"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HXZ35025.1"
                     /translation="MRFKPIRNQRGIALVLTLLILTLLVVTGLEMNRAVRVEATLAEN
                     FRDLTQASYIAQSGVEVARALIQSDNPAYDGPDETWAQFENFALLSSGFFPEGSFTGR
                     IVDENSRFNPNGLLDPYGNVNLKKREQLERLLGLLGHPVDWIEALLDWMDTDHQPRPG
                     GAERDFYLSKKKPYPAKNGPLDSLEELLLIKGVDPSLYYGGEGKEGLEGYLTVHSDGR
                     INLNTASPLMIMSLSPKIDQTMAQAVVAYRSEKSFQNPEVLRSLPGWDAVYPSISSEI
                     TVQSNYFFIEVVGNYREARALVQAVVRREGRRTRVLVWKAG"
     gene            4911..>6176
                     /gene="gspL"
                     /locus_tag="VEL68_03305"
     CDS             4911..>6176
                     /gene="gspL"
                     /locus_tag="VEL68_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HXZ35026.1"
                     /translation="MKSKTWGLDIGTTAIKAVEVTRTLRGPRLTNYAVIPLNRKEKED
                     SRAEVIRRLREVFSKLEGKGEDGVYLFPSSRTMVHRIPLPFRDRKKNQQVVKFEVEPL
                     LPFPADQVVVDFYSAEKDEAGKEALVFAARKGNLGESLSMLKEAGLDPESVVPEALAL
                     FWLVRGLGKASEGPGALLDLGQEKATLILWQGDRMTLARSIPMSGGGPTAADNPNLAR
                     LAEEVKRTFLSAGFSAGGQSVEKVYLTGGGSLAPGVDQSLSQSLGNNVSALDLGEAAG
                     EAPKEYLPVLGAALGAAWGEPSADWVNLRREEFAPVQKAQRARGRLKILISYAVILGI
                     LGGASLFLNLYLQERKYQDLKAQIRKEFSQALPEVKKVVNELQQMKARVQEEKAKLGS
                     LGGGPGGGSPLEVLRELSLMVDPGAKVRVT"
BASE COUNT         1328 a         1646 c         1899 g         1303 t
ORIGIN      
        1 acgattcatt gcccgttttc gaatacgaag gtcttagcaa ttcagggaaa gccctccgcg
       61 ggatcatcga cgcggagagc ttgcgcaacg cccggagcaa gctgcggagc cagggggtct
      121 atcccacgga aatccgggaa gaggcggccg ccgtggccct tccgggcacc tctttccgtc
      181 tcttcagcgg ggtcccgcct aaagaactgg cccgggtctt ccgccaattg gctaccctgg
      241 taggagcggg aatccctttg gtctcatcca tttccgccct gatcgatcag accggcaata
      301 ctatcctgcg caaggtcctg acccggatcc gggaaagcgt gcgggaaggg ggaagccttg
      361 ccgatgcgct ggcagcccac ccccaggtgt ttcccccgat cctggtggga ctggtgagag
      421 ccggcgaagt cagcggggca ctggccctca ccctgggccg ctgggccgac tttagcgagc
      481 atcaggtggc cttgcgccag agggtccggg ccgctctgac ctatccggtc ttcatgtttt
      541 tcatcggcgc gggggttctt tttttcctca tgaccttcgt cgttccgacg gtgacccggg
      601 tcttctccga cctgggccag tcccttccct ggcccacgct gatcctcatg gcggtgagtg
      661 atttcatgag ccggttctgg tgggcgctgg taggggcctt catcctattc gtcctctggg
      721 cgcgcaagtt tctacggagt gaaaggggag gcctgttttg ggaccgcttg aaaatgaagg
      781 tccccctggc cagggagctg caccgcaaat tggccgtatc gcgctggtcg cggaccctgg
      841 gcacgctcct gtccgggggg cttcccctgc tccaggcgct ggaatactcc cagggagtag
      901 cgggaaatcg ccttttctcc caggctatcg gccaggcgcg agatcgggtt cgggagggcg
      961 aggaaatggc tctttccctg aaaggcagtg gcctctttcc ctcggtactt ctggagatgg
     1021 tggcggtggg ggagaagagc ggagagcttt ccccgatgct ggagaaggtg gcttcaagct
     1081 tggagaatga ggcggagagc gaccttcggg gactgatcgc cctgttggag ccgcttatga
     1141 ttctcattat gggagtcgga gtgggtttca ttgccctctc cgttctcctg cccatcctgg
     1201 agatgagcca gatggtgcgt taggtctacg gaaaattcag gaccggaatc ccctctctct
     1261 ttttggtcaa aggggggaag gaaggatttt tcgggccttt ggcaaaagtg aggtgaggca
     1321 tgagatttcc cctgaaaatg gaaaatagga aggccggcgg ctttaccctg attgagctca
     1381 tggtggtgat tgtaattctg gggattctgg ccgggttggt gctcccccgt ttcatgggcc
     1441 gaactgagga ggccaagaag accaaggcca gactgcaaat tgaaaacctc gaagcagccc
     1501 tgaagctcta caaactggac aacggaacgt atcccaccac ggaacagggt ttggaatctt
     1561 tggtgctaaa gccttccacg ggaatcatcc ccaagaactg gcgcgagggg gggtatctgg
     1621 agaaatctaa ggttccggaa gacccctggg gacggcccta tgtatatatt tcccccggca
     1681 tcaaaaatcg ggactttgac ttgaagtccc tgggcgctga tggagaagag ggtggagaag
     1741 gggagaacgc ggacatcgaa aggtagcccg ggattcaccc ttgccgaact ggtcgtggtc
     1801 ctcttcctcc tgggctttat ttttcttctc acttttccca acttccgaga actgctggag
     1861 ccccgggaca tcaagagggc gacggtccga ctggcggggt ctttgcgcta cgcccagagc
     1921 caggcggccg ccaccaagca gaggtttcgc ttgaacatcg atataaagga aaacgcctac
     1981 tgggtcagcc gagagggaga gagagatacc ttcctgcggg attcctcccc catgggaaaa
     2041 cccgattacc ttcccgccgg ggtggtcttc ttagacgtga ctcacccgga gaggggcaaa
     2101 ctgcgggaag gaacggctca cattgatttc tctcccacag gctgggccga tgagtgtgag
     2161 attcatcttg gcaaaggaga agaagaggtc ttcaccattt tcattcatcc cctgggagga
     2221 aagatggaag ttagcgcagg ctacgtggag aggaaaacct cctaaaatga agaaagggtt
     2281 taccctcctc gaagtgatga tcgccctggc ccttctggct ttggtgggcg tagcctttct
     2341 ccgggcccag gccggcagcg tccgcctgct ggatgaatcg agccaaatct ccctggctac
     2401 ccttctagcc cgggagaaaa tggccgagct ggaaagcgtg gggtttcccg agttggggaa
     2461 atcttcggga acaggaggag aagcctatcc cctgttcagc tgggaacagg tcgtgacctc
     2521 cacagatctc ccggccctcc gtcaggcgtt ggtccgggtt ctctggaagg agggcacgcg
     2581 ggaacgaacc ttagagctgg tggcctactt tgcccagaaa taaaatatag tgtcagttct
     2641 tagtgtcagt acaaaaaccc gaaacccgga actcgcgaag tgaccgtgtg agccaaagaa
     2701 tgaaagcgaa cctcaagcaa tttttcaatc cgcagtccgc aatccgaaat ccgcaatcgg
     2761 gtcgcgattt tgtgtttttc aattcccaat gggcaatgcg caatgcgcaa tcggaagacc
     2821 ccgaactctt cccccgggga agcggtttta ccctcttcga agtcctgatg actctctcca
     2881 ttctgggagt cgttctttcc ctgctctatc tgacctttca tcaatccatg gcggtcatgg
     2941 ccgagaccga tgaccgggcg gaggtgatcc agcaaggccg gatgatcctg gagaggatcg
     3001 cgggagacct gaaaggggcc gtaattatag ctcagaaaga ggggttccag gcctaccgta
     3061 caggcttggt cggccgctct acgagcgagg ggaaggactt cctggaccgc atggatttca
     3121 ctaccggcct gccttctttt tcgtctcgcc ccgagggagg atggggcttt ttcgaggtgg
     3181 gctatttcct ggaccgttcc ccgggaggaa aggggttaac cctatttcga aggcaggatg
     3241 atgggatgga tggcaatctt cttcaggggg gtcggtcggt agccatctgc gaccgggttc
     3301 gaagcctggg cttctcctat tatgaccgtc agggtggaag ggggaaagaa tggaattctc
     3361 tggaagggga gcggcgcaat ctccttccct cccgggtgga gattcagtta aagctggaag
     3421 actcgcgggg aagagtacat gagttccgca cccagaccta tttgcccatg agcgagtaag
     3481 aagaagcgcg atcgattatc cacccggaaa ctccctctcc ccttgcggga aagggatggg
     3541 gtgaggggga ccctatccct tccccgttgg aggctgtgtc gtaatccgcc gctcgccctt
     3601 cgacaggctc aggcttcgtc gcgagctcag ccgagcgggc gaacggcaaa gtgggatgaa
     3661 ttttaatgcc ctcataatcc gttcgtggtg agcttgtcga accatgaacg gattatgaca
     3721 cagtctcttg agggggaggg gaaaatgcgg atttccggat ggaaactaag agcttatcct
     3781 taaatatgga tctaccttta aatccccctt tgaaaaaggg ggacataggg ggatttgctt
     3841 tagatcactt gaaaaaatcc ccccaacccc cctttgcaaa ggggggggat tattatttac
     3901 ggacaagttc taaccatgta aaaagatggt agagggaaca acaggggatg gggatgaggt
     3961 tcaaaccgat tcgcaatcaa agaggcattg ctttggtgct gacccttttg atcctgaccc
     4021 ttctggtagt gaccggcctg gaaatgaatc gggcggttcg ggtggaggcc accctggccg
     4081 agaattttcg cgacctgacc caggcctctt acatcgctca atccggagtg gaggttgccc
     4141 gggctttgat ccagagtgac aaccccgctt acgacggccc ggacgagacc tgggcccaat
     4201 tcgaaaactt cgcccttctt tcttcgggat ttttccccga gggaagtttt accgggcgga
     4261 ttgtcgacga aaacagccgg tttaacccca acgggctcct ggacccgtac gggaacgtaa
     4321 atctgaagaa aagggagcaa ctggagcgtc tcctcggcct tctgggccac cctgtggatt
     4381 ggatagaagc cttgctggac tggatggaca cggaccatca gccaaggccc gggggggcgg
     4441 aaagggattt ctacctcagc aagaaaaagc cctatccggc gaagaacggc cccttggatt
     4501 ccctggagga attacttctg atcaaagggg tggacccttc cctgtactac ggaggggagg
     4561 ggaaggaagg gctggagggt tacctaacgg tgcattccga cggaaggatc aatctcaaca
     4621 ccgccagccc gttgatgatc atgagccttt cccccaagat cgaccagacc atggcccagg
     4681 cagtggtcgc ttaccggagc gagaaatctt ttcagaaccc agaggtcctc cggtctctgc
     4741 ccgggtggga tgccgtctat ccttccatca gcagcgagat cacggtgcag agcaattatt
     4801 ttttcataga agttgtgggc aattaccgag aggcccgggc cctggtgcag gcggtagtcc
     4861 gcagagaagg aagacgaacc cgggttcttg tctggaaggc cggatagacc atgaaatcga
     4921 agacctgggg attggacatc gggacaaccg cgatcaaggc tgtggaagtc acccgaaccc
     4981 tgcgggggcc aaggctgacc aactacgcag tcattcccct aaaccggaaa gaaaaagaag
     5041 attcccgcgc cgaagtcatc cgccgccttc gagaagtttt ttccaaactg gaggggaagg
     5101 gtgaggacgg ggtgtatctc ttcccctctt cccgaaccat ggtccatcga atccccctcc
     5161 cgttccggga ccggaagaag aaccagcaag tcgtcaagtt cgaggtcgag ccattgctgc
     5221 cttttcccgc cgatcaagtg gtggtggatt tttattccgc ggaaaaagac gaagcaggga
     5281 aagaagccct ggtttttgcc gcccggaagg ggaacctggg tgagtctctg tccatgttga
     5341 aagaagcagg actggacccc gagagcgtgg ttcccgaagc cttggccctt ttctggctgg
     5401 ttcgcgggct ggggaaggct tcggaaggcc cgggggccct cctggacctg gggcaggaaa
     5461 aggccaccct gatcctatgg caaggggacc gaatgaccct ggcccggtct attcccatgt
     5521 ccgggggggg accgaccgcc gcggacaatc ccaacctagc ccgcctggct gaagaggtta
     5581 aacggacctt cctctccgcg ggattcagtg caggaggcca atccgtagag aaagtctatc
     5641 tcaccggggg cggttcattg gctcccggtg tggaccagag cttgagccag tctctgggaa
     5701 acaacgtttc cgcattggac ctgggggaag cagccggtga agcccccaag gagtacctcc
     5761 ccgttctggg ggcggccctg ggtgccgcgt ggggtgagcc ttcagccgat tgggtcaatc
     5821 ttcggcgcga ggaattcgct cctgtgcaga aagctcagag agcgagaggc cgtctcaaga
     5881 ttctgatttc ctatgccgtc attctgggaa tcctgggggg tgcatccctt tttctgaacc
     5941 tttaccttca agaaaggaaa tatcaggacc tgaaagccca aatccggaaa gagttttctc
     6001 aggctctgcc cgaggtgaag aaggtggtca atgaactcca gcagatgaag gcgcgggttc
     6061 aagaagagaa agccaagctg ggctctcttg gaggaggacc gggtggcggg tccccgctgg
     6121 aggtccttcg ggagctaagc ctgatggtag acccgggggc caaagtaagg gtgacg
//
LOCUS       DASPRR010000094        16666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_507647, whole genome shotgun sequence.
ACCESSION   DASPRR010000094 DASPRR010000000
VERSION     DASPRR010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16666)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16666
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507647"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..1533
                     /gene="dctP"
                     /locus_tag="VEL68_03310"
     CDS             502..1533
                     /gene="dctP"
                     /locus_tag="VEL68_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ35027.1"
                     /translation="MKKKLSAILLGILVAVFLASGLAFSAEAIKWKMQVAYPPGDATY
                     DVHSKETVKLIENASGGKIKIDLFAPGALCSVGEVVNAISKGMIDMGAIYGPAYAGSV
                     PVGDVEAGLPFSWMSPDQVIDLFWGSNYRMIDTIREGWAKKNIFYLYPNSCGTYPFLL
                     TYPVNKIADFKGHKIRAMGATGEWLKRAGAAPTPLPGSEIYMAIKLGTIEGTPFPPMI
                     LETLKLKEVVKYIIFPGLVAPPNTCMVINMDSWKKLPEEVKKTLTDDQKTIEVYRANG
                     KGYQARDDEALKEAYKIGIKSITLPDSEIKEGRRIAVEVWDMVGAKDTYSKKGVETLK
                     RYLTAKGLL"
     gene            1623..2138
                     /locus_tag="VEL68_03315"
     CDS             1623..2138
                     /locus_tag="VEL68_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXZ35028.1"
                     /translation="MSRILDRFIKAVNSINHWVGYITAFLLPVIILIVSYEVVMRYFF
                     NAPTIWAWDVNIQLLAVMVFLGGGYTLMQNGHVRVDLWYGKLSPRGKARADVISSPLF
                     FLFFIILLWKGGEMTLDSIAEREVMSTIFAPPLYPLKIIFTAGVFLFFLQGVVMLILD
                     LRTAFSKEKNS"
     gene            2205..3515
                     /locus_tag="VEL68_03320"
     CDS             2205..3515
                     /locus_tag="VEL68_03320"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ35029.1"
                     /translation="MSIEWLTVIMFLALLVALYLGHPLSIVLGGTAIFFTLIIWGPKG
                     LYMAATTSLASAMNETLLSVPLFVFMGTMLQASGIAEDLYDIMYRSIGRVRGGLAVGT
                     VIICTIFAAMAGISAVATITMGIIALPSMLKRGYDKHLALGCIAAGGALGILIPPSVP
                     MILYALLARESVGKLFAGGFLPGFLLSGILIAYILILCWIKPHMAPAIPPEERTRGQS
                     LLKSFKAVFWSILLIVLVLGTIYSGACTPTEAAGAGALGSVLAVVLNRKFTWNTFASA
                     IHSTFRLTAMVMWIVIGSSCFSHIFNAAGSQDLVTNLLANLPGGRWAVFCFFQLIYFI
                     LGMLLDPTGIVMICTPIFVPVITKLGFDPVWFGVTFIVNMEMAYLTPPFGYNLFYLRS
                     IAPEGVQMSDIYRSVVPFIVLQMLCLILVSIFPEIILWLPRKLV"
     gene            complement(3558..4745)
                     /locus_tag="VEL68_03325"
     CDS             complement(3558..4745)
                     /locus_tag="VEL68_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXZ35030.1"
                     /translation="MESIKDRVAIIGMGCTQFGERWDAGLNDLAIEAAYEAYQDAGVG
                     PEDIQACYMGTVTAPMIGVGGTVVADALKLRGIPIIRNENWCATGHIALIEAVTAVAS
                     GVYDVVLALGVEKLKDTGFPGLGTGRGMHPVHEARRTSPGSFSLIATRYFHQYEIDPE
                     KGKEMIGKIAVKNHYNGSLCPKAHFHNRITLEDVMKAPIIAWPLGLYDCCGNSDGSAA
                     AIVTRADLAKKFRPDPIYVKGFGIAMDSMLPHFRPGFSWTSFDALIKSSQKAYEMAGV
                     KNPREDLDIAEVHDCFTITELAIYEDFGFSPRGRAREDIDSGFFNLQGGLPVNADGGL
                     KCFGHPVGASGIRMTYEVYKQLQGKAEKPERQIKNPRRGLSHTFGGPPQLSAVAIFGN
                     EKG"
     gene            complement(4759..6177)
                     /locus_tag="VEL68_03330"
     CDS             complement(4759..6177)
                     /locus_tag="VEL68_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl CoA synthase"
                     /protein_id="HXZ35031.1"
                     /translation="MIGITSYGAYIPAFRLSRDLMAKAWLRGSIGGERSVANNDEDSA
                     TMAVEAALDCLQGKDRKDLEGLYFASTTAPYREKEISSLVAATVDLPGEILTADLGNG
                     LRAGAAALRSALDAAGNGSAKNLLVVSADCRLGYPQSEHEQSFGDGAAALLVGNSGVI
                     ATLEGHYAVSNEMMDVWRNAGDDFVRTWESRWVLGEGYSAMTEQAIKGLMKKKGIDAK
                     GIRLAAVPAPDGRTHRRMAQVLGFDKAQVVDPLLGSVGDCGAAHPLMLLVSALEAAKP
                     GDTILMAAYGNGAEAFLFKVTEEIKNLKDRRGIKGWLNSKLPLATYEKYLSYRGILET
                     QPGDPFRLLPSATAYWRDVSSILRFHGSKCRSCGTLAYPIQRVCYNCRAKDSFDEVRL
                     ADHKAKVFTFSLDNLAGRSDDPVVVQSVVESEVDRARVYCMMTDCQPKEVKIGMPVEF
                     TFRRVYEGAGFQNYFWKCRPVR"
     gene            6541..7680
                     /locus_tag="VEL68_03335"
     CDS             6541..7680
                     /locus_tag="VEL68_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HXZ35032.1"
                     /translation="MEMKILGQKELNQEVKDAHFCTGCGACVNLCPYQACYRDQVVML
                     HPCDLKEGGCYAFCPRTPTDLEGLKKSLFDPRDITLELGAVKGFYITRAADRKVRARA
                     QHGGTVTTLITLALQEGIIDSAILAEEGRNLLPQGAMVQDPGEIKKHGRSKFVVSPNV
                     AEFNRLAKEGGRKIGIVATPCQALALAKMRMKPLKNYVERIDQLKLVIGIFCGWALSW
                     GSLVEVLRKKIDLSEIEGMDIPPSKYHLLQLYTKKGTIDIPLDEVTSSVRGACWYCPD
                     LTAEFSDLSVGSARLPEGWEEAKGWNQVIVRTQLGQKLLNLAKKKGLLEFRDVPEENL
                     PKLKTASLGKKRTAVKNLKLRSRDPKNLFYLDPEDPVLRTIAAGS"
     gene            7817..9757
                     /locus_tag="VEL68_03340"
     CDS             7817..9757
                     /locus_tag="VEL68_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ35033.1"
                     /translation="MSRLIENKEGFPAVLMGNEAIVRGALEAGVDVAAGYPGTPSSEI
                     VENLSRVAGERNLYVEWSTNEKISLEVAAAASFAGLRAICPMKQNGVNVASDFLLHLA
                     GTGVRGGLVLVSCDDPGALSSINEGESRHFARMVEIPLLEPGDFQEAKDMTKWAFELS
                     EELKTVVMLRSVTRMSHASGNVVYGDLPQKKARASFKFAGPLTDIQTGPVINFPVDFR
                     HRVQHDKLKKAREIFEASPFNTYEGPKNPELLLITSSACILYTREALHLLGARDRVGV
                     LKLGTTWPLPPKLMKKHLSKTDKILIVEEVIPFLEESVKILVAEEAKEIGIKTFYGKN
                     DGTIPMTGELNPDIVITALSKILDIPYQAMAPEFEKKVQSATASLAPNRELTFCPGCP
                     HRASFWSIHAALQMDNRQGFVCGDIGCYGMGFFPCGYNTIKIAHAMGSGLGLASGFGK
                     LDSFGMDQPALAVCGDSTFFHAILPALVNAIHHKSKATLVVLDNSGTAMTGFQPHPGL
                     TIDAMGNEAPALDIARICESMGAKVETGDPFDQKETRESLNRLMEEEGVKVLILRQSC
                     ALSPEKKGKKQFQMRVDSSRCIGEECSCNRLCTRIFRCPGLVWDKEKKKARIDDVICA
                     GCGFCAAICPGGAIVREEVVRA"
     gene            9754..10371
                     /locus_tag="VEL68_03345"
     CDS             9754..10371
                     /locus_tag="VEL68_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXZ35034.1"
                     /translation="MKHLAKDPLNVIMAGVGGQGNVMASRVLANMLSLKGYKVTIGET
                     FGMSQRGGSVMSHLRVSETSAWSPQIPKGRADVLIALEPIEAVRVMRSYGNTGVKVLL
                     NTRAIHPVGVIAGDFEYPSLEGIEKGLKELTPHVWVMDATEEAMKLGNPILSNIIMIG
                     AVSGLNLLPVGMAEFKSVIRDFCPQKLLEVNRRAFEIGKEKVTSA"
     gene            complement(10500..12155)
                     /locus_tag="VEL68_03350"
     CDS             complement(10500..12155)
                     /locus_tag="VEL68_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myxococcus cysteine-rich repeat containing
                     protein"
                     /protein_id="HXZ35035.1"
                     /translation="MNRRYSKMGRASRLKLMSLLFLMAGMGTALLISPVRAALGTCGN
                     GVVDKFEQCDDGNLNDGDGCSSLCQKEGDDFPVAVYAADDPPPTPALQDLPLLTKITQ
                     HGISWTFASPQRVGRFVNGDYYIVGTATIIDIQPLPTTTNGLHGSMLNIRPNIQKSGF
                     DSRIQSGRYDPSLRVYPPITLAPGNKLVSSRSAAGSNPSSVMRPFDTSVSPVASISIL
                     TSVDAPQPLDAFRPSYAQNSSPIYLSRNLRRQLLPRLPSVQHVPPLSEFEGYLKRPWV
                     DSVFFNFDVPAEYMASYGRENAYLMSFAGLLLTLDFSPGQKEPLLVYLTQYGIDLYGL
                     VEQGHTGWQAHGGHGSGRKFPIILAGVMLNEEKMKSVRANFGEDMQTIWVSETPPAGT
                     YTKSWHSKPETVVYGGHVGINGESVNPGWGPYEHLAPSAWKNTLGESYRRCCTSVSWV
                     GEALAVRLIPGVQSAWNHPQFLAYVDRWMNTPDDPQDLATIQSATGMAIDPDFMQGQS
                     WKILSGGGYYQPHRTFVDEMWAAYRSLSLDSTPPSPPQNLRGR"
     gene            complement(12152..12535)
                     /locus_tag="VEL68_03355"
     CDS             complement(12152..12535)
                     /locus_tag="VEL68_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXZ35036.1"
                     /translation="MENTKERALFAAITLLAFLLGGCGGGSSSGTGGTGSGGTGGTGG
                     TIILDWDAPTTSADGTALTDLVGYKIHYGKASGAYDHSIDVGNVTTYVLTGLTKGQVY
                     YVAATAYDTSNNESVYSKEFQGVAK"
     gene            12942..13907
                     /locus_tag="VEL68_03360"
     CDS             12942..13907
                     /locus_tag="VEL68_03360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXZ35037.1"
                     /translation="MFPNRIMPLMIVASIHYIIPLALILWLWAGKSRSKFIWSVKTLF
                     VATYLILIYFIGFWGFASFYLRYSWFLFFALAGIFSYLKVRGVPFYTKEQAINWIWTS
                     LESCLSIFFIYLITGAIGANQYDTKPVELSFPFKNGVYSPYWGGNGKASSFMNYHYLS
                     SIHKGAKTNLSMKYAVDIEKLNNLGMSSKGFLPLELENYEIYQQEILSPCDGKVIEVV
                     DGLRDEMPWSGNYPYNVGNHVGIIKGDVCVILGHMHPGSIPVKVGDSVKTGEVIGRVG
                     SSGMADQPHIHIQAMKVSEGSIWAWEGVPIIFDGKNPVKNSLFFK"
     gene            14296..15324
                     /locus_tag="VEL68_03365"
     CDS             14296..15324
                     /locus_tag="VEL68_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXZ35038.1"
                     /translation="MLKRVKIEDAVGLKLAHDLTQIVPGQFKGAAFRRGYRIQKKDIP
                     RLLDMGKKQVYVLTLRPGELHENEGARRMARAVAGKSLRLRGPREGKIDFIARHSGLL
                     KIDTEAIARINSIGGLILATRHNDSCVQAGEIVAGTRTIPLTIPEGKVRRVEAIGKRR
                     APVIEILPFGRKKVGIVVTGSEIYEKRIKDRSAEIVKKRIERFGSRVGKSVIVPDDPK
                     RIALEIQKMKAQGCQVIVTTGGLSVDPDDRTLAGIRRSGAKVIFYGVPVLPGSMSVYA
                     RLGKTVILGAPACVVHDPTTALDLFLPRVLAGDSPTRQEMARLGHGGLCLKCPVCRYP
                     VCPFGKMG"
     gene            15329..16144
                     /gene="yqeB"
                     /locus_tag="VEL68_03370"
     CDS             15329..16144
                     /gene="yqeB"
                     /locus_tag="VEL68_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HXZ35039.1"
                     /translation="MAAKIERPSKLVILIKGGGEMATGVAHRLFHSGFLICLTEIPEP
                     LAVRREVSFCEAIYEGQKMVEGVKAIKVEGEAKVHQAWAEGLIPILVDPQCQVRQKLN
                     PDVIIDAILAKTNTGTQINDAPLVIGLGPGFRAGEDVHLVVETNRGHNLGRVIERGEA
                     EADTGVPGNIGGYTWERVLRAPSGGRFLGRKKIGDRVEKGENVAEVERLPVLSQISGV
                     LRGLLRDRGWVEKGMKVGDVDPRATREHCFMISDKARAIGGGVLEAILRRYPR"
BASE COUNT         3755 a         4486 c         4615 g         3810 t
ORIGIN      
        1 actgtagccg gctaaaaata caaaatcgag acttcagatt tcgcacaggg cctccgggcc
       61 tttagggaaa aacgggaacc tttatttaag taggaaaaac gaatccgcct tgctaagaag
      121 tccctaaacc ttaaagccaa aacgcatccc gattttgtgc taatctactt tcatcccgac
      181 acgacttttg gcatcggggc cataggcaat ctgggtcccc cggcgaaagc tttgggttcc
      241 aggaaaatct ctgttcgtta cggaaggcgg ctgcctggta gattttagga aaatttatgt
      301 tgacaaaagc cagctttctg tatacattct gccgaagatt aatttgattt ccgaatacaa
      361 tatttcagcc ctttgaccaa ataatttcaa aggaaatttg cccatcaaag gttggcctgc
      421 aaatcggcgg aattcctccc cccggggggc gcccgggaga aggaacggtc cgccccattt
      481 tttttcaaaa gggaggggaa aatgaagaaa aagttatccg caatcctttt gggaattctg
      541 gttgcagttt ttcttgccag cggtcttgcc ttttccgccg aggcaatcaa atggaaaatg
      601 caggtggcct atcctccggg ggatgcaacc tatgacgtcc attccaagga gacggtcaag
      661 ttaatcgaga acgcatccgg tggaaagatc aaaattgacc tattcgctcc gggtgcgtta
      721 tgcagcgtag gggaagtggt taacgccatt tccaagggaa tgatcgatat gggggctatt
      781 tacggcccgg cctatgcagg gtccgtccca gtgggggacg tggaagcggg gctccctttc
      841 agttggatga gtccggatca agtgatcgac ctgttctggg gctccaatta ccggatgatc
      901 gacacgatcc gggaaggctg ggccaagaag aacatttttt acttgtatcc caattcgtgc
      961 ggaacctatc ccttcctgct gacttacccg gtcaacaaga ttgccgactt taaaggtcat
     1021 aaaatcaggg ccatgggcgc cacgggagaa tggctgaaaa gagccggcgc cgctccaacc
     1081 ccactgcccg ggagcgagat ctacatggcc atcaaactcg gcaccattga aggtacccct
     1141 ttcccaccca tgatccttga aaccctcaag ctgaaagaag tggtcaagta catcatcttc
     1201 ccgggcctcg tggcgccccc caatacctgc atggtcatta atatggattc ctggaagaaa
     1261 ctgcccgagg aagtgaaaaa gaccttgacc gacgaccaga agaccatcga agtgtaccgt
     1321 gcgaacggga aaggatatca ggcaagggat gatgaagctc tcaaggaggc ttataagatc
     1381 gggattaaga gcattactct gcctgacagc gaaatcaagg agggccgtcg cattgcggtt
     1441 gaggtttggg atatggtggg cgctaaggac acctattcca agaagggcgt cgagaccttg
     1501 aagcgttacc tgaccgccaa aggccttctg taagtaatcc ccgatctctg cttatcttgc
     1561 gcggccctgc cgggggccgc gcaagataaa aaggcaaccc catgaatata gagggggaat
     1621 ccatgtcacg aatcctcgat cgattcatta aagcagtcaa ttcaattaat cattgggtgg
     1681 gctacattac ggctttcctt ttgccggtga tcatcctcat tgtctcctat gaagtggtca
     1741 tgcgttattt cttcaatgcc cccacaatct gggcctggga tgtgaacatt caactcctcg
     1801 ccgtcatggt tttcctgggg ggcgggtaca ccctcatgca aaacgggcac gtaagagtgg
     1861 atctgtggta cggcaaattg tctcctcggg gaaaggccag ggctgatgtg atcagttcgc
     1921 cactgttctt ccttttcttt atcatccttc tgtggaaagg cggggagatg acgttggact
     1981 ccattgccga gcgcgaagtg atgtccacca tttttgcccc cccgctctat cccctgaaaa
     2041 tcatctttac tgcaggggta ttcctgttct ttctgcaggg cgtggtcatg ttgatccttg
     2101 acctgcggac ggctttttcg aaggaaaaaa attcctaaaa aaccgtcaaa actctgaatg
     2161 ggatgagatc atttgactga gcttgttggc ggagagggtt ttttatgagt atcgaatggt
     2221 taacggtgat tatgtttctg gccttattgg tcgctctcta tttgggccac ccgctttcca
     2281 tcgtcctggg cgggacggcc atattcttca ccctcatcat ctgggggccc aagggacttt
     2341 atatggcggc cacgacctcc ctggcgtcgg ccatgaatga aactcttctt tccgttcccc
     2401 tgttcgtctt catgggtacc atgctccagg cctcggggat cgccgaggat ttatatgata
     2461 tcatgtaccg gtctatcggc cgggttcggg gggggctggc ggtgggcacg gtgatcatct
     2521 gcaccatctt tgcggccatg gccggcatca gcgccgtcgc caccattacc atggggatca
     2581 tcgccctgcc ctccatgctc aagcgtggtt acgataagca tctggccttg ggctgcatcg
     2641 cggcgggagg ggctctgggg atcctcatcc cccctagcgt ccccatgatc ctttatgcct
     2701 tactcgccag ggagtcggtt gggaagcttt ttgcgggggg gtttctccca ggtttcttac
     2761 tctcggggat tttgatcgcc tatatcctga tcctctgctg gatcaagccc catatggccc
     2821 cggcgatccc cccggaggaa cgaacccggg ggcagagttt gctgaaatct ttcaaggctg
     2881 tcttctggtc catccttctc atcgtgctgg tattggggac tatttatagc ggcgcatgta
     2941 cccccacgga agccgcggga gcaggagccc tgggcagtgt tctagccgtc gtcctcaaca
     3001 gaaaatttac ctggaacact tttgcttcgg ccatccactc cactttccgc ttgaccgcca
     3061 tggtgatgtg gatcgtcatc gggagcagct gtttttccca catctttaat gccgcggggt
     3121 cacaggacct agtgaccaat cttttggcta acctgcccgg gggccgttgg gctgtctttt
     3181 gtttcttcca gcttatctat ttcatcctgg ggatgctcct ggacccaacg ggaatcgtta
     3241 tgatctgcac tcccatattc gttccggtca ttactaaatt gggttttgat cccgtctggt
     3301 tcggggtgac ctttatcgtc aacatggaga tggcttatct gaccccgcct tttggctata
     3361 acctttttta ccttcgaagc atcgcccccg aaggggtcca gatgtcagat atttataggt
     3421 ccgtggttcc cttcatcgtt cttcaaatgc tctgtctgat cttggtttcc atttttcccg
     3481 agatcattct ctggctgccc agaaaattgg tatgaaccaa gggcgttaat cgccgatctc
     3541 ccctgctggc ccgagattta tcccttctcg tttccgaaga tggccacggc gctcagctgg
     3601 ggcgggcctc cgaaggtgtg ggagaggccc cggcggggat ttttgatctg ccgttccggt
     3661 ttctccgcct taccctgaag ctgtttgtac acttcatagg tcatgcgaat cccgctcgcc
     3721 cccaccggat ggccaaagca tttcagccca ccgtcggcgt tcaccggcag gcccccctga
     3781 aggttaaaga agcccgaatc gatgtcttcc cgagcccttc ccctcgggct gaatccgaag
     3841 tcttcataga tcgccagctc ggtgatggtg aaacagtcgt gcacctcggc aatgtccagg
     3901 tcctcccggg gatttttgac cccggccatc tcgtaggctt tctgggacga cttgatcagg
     3961 gcatcgaaac tggtccagct gaatcccggc cggaaatggg gaagcatgga atccatggca
     4021 atgccgaatc ccttgacgta gatggggtcc ggccggaatt tcttggccag gtccgcccgg
     4081 gtgacaatgg cagcagccga tccatcgctg tttccgcagc agtcataaag gcccaggggc
     4141 caggcaatga tgggtgcctt catcacatct tccagggtga tccggttgtg aaaatgggcc
     4201 ttggggcata ggcttccgtt gtaatggttc ttcaccgcaa tcttcccgat catctccttg
     4261 cccttttccg ggtcgatttc atactggtgg aaatagcgcg tggcaatgag ggaaaaggac
     4321 cctggggagg tcctcctggc ctcatggaca ggatgcatgc cccgtcccgt cccaaggccg
     4381 ggaaatcccg tgtccttcag cttctccact cccagagcca agaccacatc atagacgccg
     4441 ctagccactg ccgttaccgc ttcgatgagg gcgatgtggc cggtggcgca ccagttttca
     4501 ttccggatga tgggaattcc ccgcagcttc agcgcatccg ccaccacggt cccaccgacc
     4561 ccgatcatgg gcgcggtcac cgttcccata tagcatgcct ggatgtcttc cgggccgacg
     4621 ccggcgtcct ggtaggcctc gtaggccgcc tcgatggcca ggtcgttcag cccggcatcc
     4681 cacctttctc caaattgggt gcatcccata ccgatgatgg cgactcgatc ctttatgctt
     4741 tccatggcct tttcctcctt atctcaccgg ccggcatttc cagaagtagt tttggaaacc
     4801 cgccccttca tagacccgcc ggaaagtgaa ttccaccggc attccaattt tcacctcttt
     4861 aggctggcag tcagtcatca tgcagtagac ccgggctcgg tccacctccg attcaaccac
     4921 gctctggacc actaccgggt cgtcgctccg cccggccagg ttgtccaggg aaaaggtgaa
     4981 gaccttggct ttgtggtccg cgaggcggac ttcatcgaaa gaatcctttg cccggcaatt
     5041 gtaacagacc ctctggattg gataggccag ggtcccgcaa ctgcggcatt tgctgccgtg
     5101 gaagcggagg atggagctca cgtctctcca ataggccgtg gccgagggaa gaagccggaa
     5161 aggatcgccc ggctgggttt ccaggatgcc ccggtagctg aggtattttt cataggtggc
     5221 caggggaagc ttggaattca gccagccctt gattcccctg cgatctttaa ggttcttgat
     5281 ctcctcggtc accttgaaga gaaacgcctc cgctccattg ccataagcag ccattaatat
     5341 ggtgtccccc ggcttagctg cttccagcgc ggaaacgaga agcatcagcg gatgggcggc
     5401 cccgcagtcg cccacgctcc ccagcaacgg atcgacgacc tgggctttgt cgaaccccaa
     5461 gacctgggcc atgcgccggt gggttcggcc atcgggcgcc gggaccgcgg ccagcctgat
     5521 ccctttggca tcgatcccct tcttcttcat taatcccttg atggcctgct cggtcatggc
     5581 ggagtatccc tcccccagga cccatcggct ctcccaggtg cgcacgaagt catcccccgc
     5641 gttcctccaa acgtccatca tctcattgga aacggcataa tgcccttcca gggtcgcaat
     5701 gaccccggaa tttcccacca gcagggccgc ggccccgtcg ccgaaggact gctcatgctc
     5761 cgactgggga tacccgaggc ggcaatcagc ggagacgacc aaaaggtttt tggccgagcc
     5821 gtttcctgcg gcatccaaag ccgaacgcaa ggccgccgct cccgctctca gtccgttgcc
     5881 caggtccgcg gtcaggatct ctccgggcag atcgaccgtg gcagcaacca aggaggaaat
     5941 ctccttctcg cgataggggg ccgtggtcga ggcgaaatag agcccctcca ggtctttgcg
     6001 gtcctttccc tggagacaat cgagggccgc ctctacggcc atggtggcgc tgtcctcatc
     6061 gttgttggcc acactccttt cccccccgat tgaacctctc agccaagcct tggccattaa
     6121 atcccggctc aatcggaatg caggaatgta agccccatag gatgtaatcc cgatcatgga
     6181 ttcagcctcc cttctctaga ctataaagat gtaagcggta agttctgttt aggcccggtt
     6241 ccgccggcca aaatttcttt ccttgttttg tatacataaa atctcggaaa aatgcaatag
     6301 gagtctacaa aaaatattcg aagaccgagc tggttttggg gggtatgttt tgacaaagaa
     6361 ttgccctctc cgttaagacc ggaagtgatt ttttcaccac gggtcctcaa agtttgctat
     6421 ataataaggc aactgccaga gcgggcttat ggatcaccaa cgggaaaaag ataccttctt
     6481 tttttggttt tcctgtccac cctgaatccg gcatttcaat tttcggcgga ggaaagcgca
     6541 atggaaatga aaatcctggg tcaaaaggag ctcaaccagg aggtaaagga tgctcacttc
     6601 tgcacgggtt gcggggcctg cgtcaacctg tgcccctacc aggcttgcta ccgggatcag
     6661 gtagtcatgc ttcatccctg cgacctaaag gaaggggggt gctacgcctt ctgcccacgg
     6721 acgcccaccg acctggaggg tctcaagaag agcctttttg atccgagaga tataaccctt
     6781 gaactcgggg cggtgaaagg cttttacatc acccgggctg cggaccggaa ggtccgggcg
     6841 agagcgcaac acggcgggac cgtaacgacg ctgatcaccc tggcccttca ggaagggatc
     6901 atcgattcgg ccattctcgc ggaggaaggc cggaatcttc tgcctcaggg tgcgatggtg
     6961 caggacccgg gggagatcaa gaagcatggc cgcagcaaat tcgtggtctc ccccaatgtg
     7021 gccgaattca accggttggc caaggagggg gggcggaaaa ttgggatcgt ggccacccct
     7081 tgccaggccc tggccctggc caagatgcgg atgaagcccc tcaagaatta cgtggaacgg
     7141 atcgaccagc tgaaactggt catcgggatc ttctgcggat gggccttgtc ctgggggagc
     7201 ctggtggaag tgctccggaa gaagattgac ctctcggaaa tcgagggcat ggatatcccc
     7261 ccgagcaagt atcaccttct gcagctttac accaaaaagg ggacgattga tattccgctt
     7321 gatgaggtga cctcctccgt gcggggagcc tgctggtact gccccgatct gaccgccgag
     7381 ttcagcgatc tttcggtggg atcggcccgc ttgcccgaag gttgggaaga ggccaaaggc
     7441 tggaatcagg taatcgtgcg gacccagttg gggcagaagc ttttgaacct ggcgaagaag
     7501 aaggggcttc tggagtttcg cgatgtgccc gaggagaatc tgcccaaact gaagaccgcc
     7561 tccctgggaa agaagaggac ggcggtcaag aacctgaaac tcaggagccg cgaccccaag
     7621 aacctgttct atctggaccc ggaggaccct gttttgagga ccattgccgc tggttcctag
     7681 gccaaaatga ccccgaattg tgagggaacc cctgggtggt tgcccagctt gcgggttgcc
     7741 acatggggcc gcccttacaa catcctgttt ggttccggct tggccgggtt ggttttacca
     7801 gaaataggag gacaacatgt cgagattgat cgaaaataaa gaagggtttc ctgctgtgct
     7861 catgggtaac gaagccattg tccggggggc cctggaagcg ggggtcgatg tggccgcggg
     7921 ttatccaggc accccgtctt ccgaaatcgt ggagaacctg tcccgggtcg ccggggagag
     7981 aaacctgtac gtggaatggt cgaccaacga gaaaatctcc cttgaggtgg ccgcagccgc
     8041 ttcctttgcc ggtctgcgcg ccatctgtcc catgaaacaa aacggagtga acgtggcctc
     8101 cgatttcctc ctccacctgg ccggcacggg tgtgcggggg ggactcgtcc tcgtctcctg
     8161 cgatgacccc ggggctcttt ccagcatcaa cgagggggag tcgaggcatt tcgcccggat
     8221 ggtggaaatt cccctgctgg agcccgggga tttccaggaa gccaaggaca tgaccaaatg
     8281 ggccttcgaa ctctccgaag agctcaagac cgtggtgatg ttgcgcagcg tcacccgcat
     8341 gtctcacgcc agtgggaatg tggtttacgg agacctgccg cagaaaaagg ccagggcctc
     8401 cttcaagttc gccgggcccc tcacggatat ccagaccggc ccggtgatca acttccccgt
     8461 ggacttccgg caccgtgttc agcacgataa actcaagaag gccagggaga ttttcgaagc
     8521 gagcccgttc aacacctatg aaggcccgaa aaaccccgag ttgctcctca tcaccagcag
     8581 cgcctgcatc ctttataccc gggaggccct ccacctcctg ggggcccgag accgggtggg
     8641 ggtgctgaaa ctgggaacca cctggcccct gccgcccaaa ttgatgaaga agcacctttc
     8701 caagaccgat aaaatcctga tcgtggagga ggtgatcccc ttcctggagg aaagcgtaaa
     8761 gatcctcgtc gccgaagagg ccaaggaaat cgggatcaag actttctacg ggaaaaatga
     8821 cggcacgatt cccatgaccg gggagctcaa tccggacatc gtcatcacgg ccctatcgaa
     8881 aatcctggat attccctacc aggccatggc cccagaattc gagaaaaagg tccagtcagc
     8941 caccgcctcc ctggctccca accgggaatt gaccttctgc ccgggatgcc ctcatcgcgc
     9001 ctccttctgg agcatccatg cggccctgca gatggataac cgacagggat ttgtgtgcgg
     9061 ggatatcggc tgctacggta tggggttctt cccctgcggc tataacacca tcaaaatcgc
     9121 ccatgccatg ggctccgggc tgggcctggc cagcgggttc ggaaagctgg attccttcgg
     9181 gatggatcag ccggccttgg cagtgtgcgg ggattccact ttcttccatg ccatattgcc
     9241 cgccctggtc aatgccattc accataagtc gaaggcgacg ctggtcgtcc tggacaacag
     9301 cgggacggcc atgaccggtt tccagcctca tccaggttta accattgatg ctatgggaaa
     9361 tgaagccccc gccctggaca tcgcccggat atgcgaatcc atgggggcca aagtggaaac
     9421 cggcgatccc tttgaccaga aggagacccg ggagtccctc aaccgcctga tggaggaaga
     9481 aggagtaaag gtcctcatcc ttcgccagag ctgcgccttg agcccggaga aaaaaggcaa
     9541 gaagcaattc cagatgcggg tcgattcctc ccgatgcatc ggggaagagt gcagctgcaa
     9601 ccgcctgtgc acccggattt tccgttgccc gggcctggtg tgggacaagg aaaagaagaa
     9661 agcccgcatc gatgacgtga tttgcgcggg gtgcgggttc tgcgcggcca tctgtccggg
     9721 cggtgccatc gtgagagagg aggtggttcg cgcatgaagc atttagccaa agaccccttg
     9781 aatgtcatca tggccggggt ggggggccag ggaaacgtca tggcctcccg cgtcctggcc
     9841 aacatgttgt ctttgaaagg gtataaggtg acgatcggcg aaaccttcgg catgagccag
     9901 cggggcgggt cggtcatgag ccacctccgg gtgtcggaga cctccgcatg gagccctcaa
     9961 attcccaagg gccgggccga tgttcttatc gccctggagc ccatcgaggc tgtccgggtc
    10021 atgcggtctt acggcaatac cggggtgaag gtgctgctga atacaagagc catccacccc
    10081 gtgggggtca ttgccgggga ttttgagtac ccatcactgg agggcattga aaaggggctg
    10141 aaggagctta ccccccatgt ctgggtgatg gatgccacgg aagaagcgat gaaattgggg
    10201 aatcccatcc tcagcaacat catcatgatc ggagcggtca gcggacttaa cctcttgccc
    10261 gtaggaatgg ccgaatttaa gagtgtcatc cgcgatttct gcccccaaaa actcctggag
    10321 gtcaaccggc gggccttcga aatcgggaag gaaaaggtga cgtccgctta ggcgcttatc
    10381 gcgccctcca taaagcccta ttggcacgag cgggacggcc tgaaggacgc cgtagggctt
    10441 gatgcatcgt gccccttcag acggagctgg cttctcaggg aagggaggct gctttctgat
    10501 caccgccccc ttaaattttg gggaggcgaa gggggagtgg agtccaggga gaggctccga
    10561 taagcagccc acatctcatc gacgaaggtg cggtgaggct ggtagtagcc tcccccgctt
    10621 agaatcttcc atgactggcc ctgcataaag tcaggatcaa tggccatgcc ggttgcgctt
    10681 tgaatggttg ccaggtcctg cggatcatcg ggggtgttca tccaccgatc cacataggcc
    10741 aaaaattgag gatgattcca ggccgattga actcccggaa tgagtcgaac ggcgagcgct
    10801 tctcccaccc aactcacgct ggtgcaacat cgacggtaag attctcccag cgtattcttc
    10861 caggccgaag gcgctaaatg ctcgtagggt ccccagcccg ggttcaccga ctcaccgttg
    10921 attcccacat gccccccgta aacaacggtt tccggcttgc tgtgccagga ttttgtgtag
    10981 gtgcctgcgg gaggcgtttc gctcacccaa atggtctgca tatcttcccc gaagttggct
    11041 cgaaccgatt tcattttttc ttcattcagc atcaccccgg ccagaataat gggaaacttc
    11101 cgtccactgc catgtccccc atgagcctgc cacccggtgt gaccttgctc gacaaggccg
    11161 tacaaatcga ttccgtattg agtaagatac acgagtagag gctctttttg tcccggagaa
    11221 aaatccaaag tgaggagaag accggcaaaa gacatgaggt aggcgttctc tcgtccataa
    11281 cttgccatgt attcagccgg aacatcaaaa ttgaaaaaga cagaatccac ccatggccgt
    11341 ttcaaatacc cttcaaactc ggacagggga ggaacatgtt gcaccgatgg aagtcggggg
    11401 aggagctgcc gccgcaaatt tcgggacaaa tagatggggc tggaattttg ggcataagag
    11461 gggcgaaaag catccagggg ttgcggagcg tccacgctgg tcaaaatgga aatcgaggcc
    11521 acggggctga ccgaggtgtc aaaagggcgc atcaccgagg atgggttgga gcctgccgcg
    11581 ctgcgggagg ataccaattt atttccaggg gccaaggtga tcggaggata aacgcgcaaa
    11641 cttggatcgt accggccaga ctggatgcgg ctatcaaatc cactcttttg gatattgggc
    11701 cgaatattca gcatcgatcc atgcaatcca ttggtcgtcg tgggcaaagg ctggatatcg
    11761 atgatggtgg ccgttcccac tatatagtaa tctccgttca caaaacggcc caccctttgc
    11821 gggctggcga atgtccagga aatgccgtgt tgagtaatct tggtgagaag gggcaaatcc
    11881 tgaagggctg gcgtaggggg cggatcgtcc gctgcgtaga cggccaccgg aaagtcatcc
    11941 ccttcctttt gacagaggct tgagcatcca tctccatcgt tcaaattgcc atcgtcacac
    12001 tgctcaaatt tatccaccac gccattcccg cacgttccaa gggccgccct aaccggcgag
    12061 atcaaaagag cggttcccat ccccgccatc aaaaagagaa gagacatgag tttcaacctg
    12121 gatgctcttc ccatcttcga atatctacga ttcatttcgc aaccccttgg aattctttcg
    12181 aataaacact ctcattgttg gaggtatcat aggcagtcgc tgcgacataa tagacctgcc
    12241 ctttcgtaag gcctgtcaaa acataagtcg tcacattccc aacatcaatg gaatgatcat
    12301 aggctccgga ggcttttcca taatgaattt tataaccaac cagatcagtc aaggcggttc
    12361 cgtcggcact cgtggtcgga gcgtcccagt ccaaaatgat cgtcccgcca gttcctcccg
    12421 ttccaccact tccagtccca ccagttccgg aggaactccc gcccccgcat ccacctaata
    12481 ggaaggccaa caaagtaatg gctgcgaata aggctctttc tttcgtattt tccattctat
    12541 ttcctttttt tatcttcgat ccgagccctt accaggtgca atttccctgc catggactat
    12601 ggacaggtaa gagaggggta acgaagctaa ctaaaagaaa atgtatggat ttttcaaaag
    12661 tttttcatgg cgaaatagat tgctggtaga gtgaacagag caaaaatgtc aataagtggt
    12721 cgattttggg ggagattatc aaaaatacag tgatgatggc tctggaatgg gagagtcaag
    12781 aaccctcccg atcctcctcc caagaattgc gttaatcgtt atgactctta gccggccgat
    12841 agagtgtaaa ctatgaccct tgtcatgcaa aaaatggcgc caaattcata gcataaattg
    12901 tttgcagttc attagcaggg taacacccat tggagtttga catgtttccg aatcgtatta
    12961 tgccgttaat gattgtagca tcgattcact atattatccc cttagcccta atcctttggc
    13021 tttgggcagg gaaaagcaga tcgaaattta tctggtccgt aaagaccctg tttgtcgcca
    13081 cgtatcttat ccttatctac ttcattggct tttggggctt cgccagtttc tacttgagat
    13141 attcctggtt cttgtttttt gcccttgcgg ggatcttctc ctacttgaaa gtccgggggg
    13201 taccatttta tacgaaggag caggcgataa attggatttg gacttcactc gaatcctgtt
    13261 tgtctatctt cttcatatat cttattaccg gcgctattgg ggcaaatcaa tacgatacca
    13321 agcccgtgga actttcattc cccttcaaga acggcgtgta ttcaccctat tggggtggga
    13381 atgggaaagc aagttcgttc atgaattatc attatctgtc ctcgatacac aaaggggcca
    13441 agacaaatct gtcgatgaaa tatgccgttg atattgaaaa attaaataac ctggggatgt
    13501 cttcaaaagg ctttcttccg cttgaattgg aaaattatga gatatatcag caagaaattc
    13561 tttctccctg cgatggtaaa gtgatagaag tcgtcgatgg actgcgggat gagatgccct
    13621 ggtccggtaa ttacccctat aatgtcggga atcatgtagg gattataaaa ggggacgttt
    13681 gcgtcatctt ggggcatatg cacccgggga gcattcccgt taaggtgggg gattctgtaa
    13741 aaacggggga ggtcatcggg agagtgggta gttcaggaat ggccgaccag ccccatatcc
    13801 acattcaagc catgaaggtg tcagaaggct ctatttgggc gtgggaaggg gttcctatta
    13861 tatttgatgg aaagaatcct gtcaaaaata gcttattctt taagtgattt gaacctatca
    13921 tgcggaggaa agctcgggaa acctctcgcc taaattgctg aattgatgtc gatcgtatgg
    13981 gtgggcactg cttctttgcc agccccctga ttcttttccc gtatcttcaa ccccttcaaa
    14041 tcccctgaac cttcttcctc tccccctccc aatccccgaa tctggacttg agcggaaaca
    14101 tgagataata taaaatctag caggatgcgg aaaaactttt tttcagccct tgatgtcatt
    14161 cccgcgaaag cgggaatcca gtcgtttcaa agccttctgg gctcccgctt tcgcaagagt
    14221 ggccgctttt cggagttttt ccgcatcctg ctcgggccaa ggggaaggaa aaaatcgcga
    14281 ttcaagggaa ttcggatgct caagagagtc aaaatcgaag atgcggtggg attgaagctg
    14341 gcccacgatt taacccagat cgttccgggc caattcaaag gtgcggcctt tcgccgaggg
    14401 tatcgcatcc aaaagaaaga tatccccagg ctgctcgaca tggggaagaa gcaagtctat
    14461 gttttgactt tgcgccccgg agagttgcac gaaaatgagg gtgctcggcg gatggcccgg
    14521 gccgtggccg gcaagagtct tcggctccgg ggcccgaggg aaggaaagat agatttcata
    14581 gccagacatt cggggctttt aaaaatcgat accgaagcga ttgcccggat taattccatc
    14641 ggaggtctga tcctggccac ccgacacaat gattcctgcg ttcaggcggg agaaatcgtg
    14701 gccgggacgc gcaccatccc ccttacaatt cccgaaggga aggtgaggcg ggttgaggcg
    14761 attggaaaga gaagagcacc ggtgatcgaa attctgccct ttggcagaaa aaaagtcggc
    14821 atcgtggtca ccggcagtga gatttatgaa aagagaatca aggatcgttc ggcggagata
    14881 gtaaagaaaa gaatcgagag gttcggttcc cgggttggga aatcggtcat cgtgccggat
    14941 gaccccaaaa ggattgccct cgaaatccaa aagatgaaag cccagggttg ccaggtcatt
    15001 gtgaccaccg gcggtctttc ggtagacccc gatgatcgga ccttggctgg aatccgaagg
    15061 tcgggcgcca aggtcatttt ctatggggtc cccgtcctgc ccggctccat gtcggtttac
    15121 gcccggctgg ggaaaacggt catcctgggg gcccctgcct gtgtcgttca tgaccccacc
    15181 actgctttgg atttattcct tccaagggtt ctggccgggg attctccaac ccggcaagaa
    15241 atggctcgac tgggccatgg ggggctgtgc ctgaaatgtc ccgtttgccg ctacccggtc
    15301 tgcccttttg gaaaaatggg gtgaacccat ggccgcgaag atcgaaagac cttccaagct
    15361 cgtgatcctg atcaagggag gaggagagat ggccaccgga gtcgctcatc gcctcttcca
    15421 ttccggtttc ctgatttgcc tgacggagat tcccgagcct ctggctgtgc ggcgagaagt
    15481 ttctttctgt gaagccatct atgagggtca aaaaatggtg gaaggggtca aagccataaa
    15541 ggttgaagga gaagccaagg tccaccaggc ctgggctgaa ggcttgattc ccatccttgt
    15601 ggacccccag tgccaggtcc gccagaaatt gaacccggat gtaattatcg atgccattct
    15661 cgccaagacg aataccggca cgcaaatcaa tgatgcccct ttagtgatcg gccttggccc
    15721 gggatttcgc gcaggagaag atgtgcatct ggtcgtggaa acgaatcgag ggcacaacct
    15781 gggcagggtt atcgaacgcg gggaagcgga agcggacaca ggggttcctg gaaatattgg
    15841 cgggtacacc tgggaacggg tcttacgcgc tccgtccgga ggaaggtttc tgggcaggaa
    15901 aaagatcggg gaccgcgtgg aaaaagggga gaacgttgcc gaagtagaaa gactcccggt
    15961 cctctcacaa atctccgggg ttctgcgagg cctgttgcgc gaccgagggt gggttgaaaa
    16021 aggcatgaag gtgggcgacg tggacccgcg ggccacccgg gaacattgct ttatgatttc
    16081 ggacaaagcc cgggccatcg gggggggagt tctcgaagcc attttgagac gatatccccg
    16141 ataaccggaa gtggatgacc gatttgaaat atagacccct gctaaaaaaa ggttctgttc
    16201 gaaaagtacc tgactaagcg ctttttgcga attggcagag ccgcacccag cagccaggat
    16261 tttccttgga tcacggccct gccagaaatc ccccctaccc ccctttatca aaggggggat
    16321 tgggggggat ttcagagaag ggttgaccaa ataagatttc ttggcaaatt tcaagcattt
    16381 ttattttgac cgccccgcgc gacggatcac gtaatccgcg aacagaggcc caaaatgcac
    16441 ttaaggattt cgccgggaaa tgagggcccg agtctgtttc cgggcagcca ggggttgggc
    16501 tcgaaacttt ttctgggggg ttaccatggg ggatttattc gatgaggtcc aaaagctcaa
    16561 atcggaaggg aaaaaagggg ccttggccac gatcataggg accaagggat cgaccccccg
    16621 cgaggtggga gccaaaatgc ttctccggga agacgggacc ctcctg
//
LOCUS       DASPRR010000095         8108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_675185, whole genome shotgun sequence.
ACCESSION   DASPRR010000095 DASPRR010000000
VERSION     DASPRR010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675185"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VEL68_03375"
     CDS             <1..434
                     /locus_tag="VEL68_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HXZ35040.1"
                     /translation="HGFIYDSSMMGNDFTPYRVRQGDLINLDQAAVFGKTTRLIEIPV
                     SWTLDDHPHFEFIRTKEWVLQGLMNANLVLDDWISDFNYMKKKLEWGVIIYTFHPYVI
                     GRGGRMLILEKLIRKLRDKGVVFTTVEAAAAEYEKRQPFKG"
     gene            504..1058
                     /locus_tag="VEL68_03380"
     CDS             504..1058
                     /locus_tag="VEL68_03380"
                     /inference="COORDINATES: protein motif:HMM:NF021439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HXZ35041.1"
                     /translation="MPILDGREQERKGVIETANLMVVSARTAPKSGGVDDILTAIVYG
                     AEVGALAADMEKLAVERKIERWSKQAQEIKGSEVIVLIGVRGTKRYVTDCGACGYKSC
                     DDFEKAEKKLGQDFEGPTCIFKALDLGIAVCSAVKTASLLNVDNRIFYRIGAAAKRLN
                     YLPEASLIMGIPLSSEGKNPYFSR"
     gene            complement(1597..2886)
                     /locus_tag="VEL68_03385"
     CDS             complement(1597..2886)
                     /locus_tag="VEL68_03385"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ35042.1"
                     /translation="MEALLISFFLLMIIGAPIGVALGASSVIFVIFYTSMDPVFVPSA
                     FLYFMHHYTIMAVPFFIYAGYLMEKTGVVKLLFDLSEALLSWVPGGFGVATLGCCVIF
                     AAITGSSVAEASAMTVIAVPEMIKRGYSPKLAAGIVCSGGTLGLLIPPSLSLLMFGVV
                     TDTSVAKLFFAGFIPGFILAGCLMAVTVVIGIKQKLPTGPFDPGKAMVSLWRCLPGLA
                     FPVIVLGGLYGGFFTPTEAGAAACGYALLYGTLARRKAFLKELIPSTVLTVRLTSVVF
                     FLLGSVGIFQAVAANEYWPQKIAEAVAAMGLSPLTFLFGYMIFILILGCFMDGMGMIL
                     LTIPVVFPIAKALGVDPLHFGVLMTINVELGTITPPVGFNLYAVSGIGKISVDQVLRG
                     VLPFFLTMLAFLFFMIFMPDLCTWLPGLIFKPIQFGG"
     gene            complement(2963..3508)
                     /locus_tag="VEL68_03390"
     CDS             complement(2963..3508)
                     /locus_tag="VEL68_03390"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ35043.1"
                     /translation="MEKAYQYWKWFQDRFLAPITAVVFLGPILLACVEVIRRYFFGES
                     WDWQQDVVTYFILSATYLFFSITERQGMHLKLTLFTNLFSKKVHPGMGHAMRLLAQLA
                     SVLYLGYFTTYGVKITQTTYSSGRLVLSQSMVFWPFFLILTIGMGLMIISFVFEIFRE
                     VQAMRGKQVLVEEAASAHSTD"
     gene            complement(3683..4810)
                     /locus_tag="VEL68_03395"
     CDS             complement(3683..4810)
                     /locus_tag="VEL68_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ35044.1"
                     /translation="MTMTKRISIISLALVFLVGFLSPVSAQTGKKMVIRIVSDTSPPP
                     QPIAISMNWFRDKIKERIPGSEVRTYFAGALYAMSDAMGALQAANLEIAVGQPSKVAG
                     FDPWLNIATQPMVLTSIGAIHHFPETDVAKALSERLSKKGILVMGWSDISHYMGVASK
                     KRIFVPADFKGLKLRVLAPLTQGPTLKAWGASAVAMAWGDVPSAIQSGVIDGALTTTN
                     GFAVIKEQASYYTIISVGGMALDFYFLGFSKKWWDGVASEHQKIIKDTLNEMILYQQK
                     LQWCSDTLVAEDFGTKDPKKPGMYNLKPEQVEPFKQALGTKVAEALIEKLGAESKSLI
                     FKFQEEGNKLVAKYPIGSDPVESMDCAPYKKLVTYQEEKKK"
     gene            5299..7152
                     /locus_tag="VEL68_03400"
     CDS             5299..7152
                     /locus_tag="VEL68_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ35045.1"
                     /translation="MGEQVLEGYMGKVLRVDLTRGVIKEEPLEKELVESYVGGTGLGT
                     EYLYREVPPGVSWDAPENRIIMASGPLGGTTVSGTGTFSLVTKGPMTNLAVSTQANGF
                     WGAYLKFAGYDAVIIQGRADRWKYLYIGEGRVELRDASALVGKDTWETEEILRRELGA
                     GQRLSVFGIGPAGEKLVRFAVVAGDQGHIASKNGSGCVMGSKRLKAIAISREQGSVPI
                     SNKSLLAEKVKALDLDAKENRGGQMYKWGTGGGFSGHALAGSLPIRNYTTNVFPEHEK
                     MSGQYIRSHFEYRNKPCWACRLVHTKYMKVTEGAYAGAEGEEPEYECLAAWGPVIGNT
                     DPGAVIVLSDLTDRLGMDVNEAGWTIGWVMECYEKGLLSRGDLDGLEMRWGNVEAVRE
                     MLKKISQREGIGDLLAEGVKRASEKIGGEALKMGVYVQKGATPRGHDHRAIWAEMLDT
                     CVSATGTIQSVSRMVPLPVFGHPPVSNPFSPWEVAGASAKAEGWYVFMDCLGICRFTA
                     IDPKLTLDCVNAVTGRGLTFSDALIIGRRIINQLRVFNFRHGLDPALEIPSARYGSAP
                     VDGPAKGKAIGPHFEWMKRFYFELMGWDPETGKPLPHTLKSLGLEKLIEAF"
     gene            7245..7478
                     /locus_tag="VEL68_03405"
     CDS             7245..7478
                     /locus_tag="VEL68_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ35046.1"
                     /translation="MMMEEEIQEDESLRSLLNRLAGRLPQLPVPIFDPETQTLSSEVA
                     VVINDHLHHFSQGLETKLKDGDRILIFPYLAGG"
BASE COUNT         2010 a         1907 c         2381 g         1810 t
ORIGIN      
        1 agcatggttt catttacgac agcagcatga tgggaaacga tttcaccccc taccgtgtgc
       61 gccagggcga tttgatcaat ctcgatcaag cggcagtatt tggcaagacc acccgattga
      121 ttgagatacc ggtcagctgg accctcgacg accatcccca ttttgaattc atccgcacca
      181 aggaatgggt tcttcagggg ctgatgaacg caaacttggt actcgatgat tggatcagcg
      241 atttcaacta catgaaaaag aaattggaat ggggggtaat catctacacc ttccatccct
      301 acgtgatcgg gcggggcggg cgtatgttga tcctggaaaa gctgattaga aagctaagag
      361 ataaaggggt tgtgtttaca accgtcgagg ctgctgctgc tgaatacgaa aaaagacaac
      421 ccttcaaagg gtgatgctga gagaaagcgg tattggagga ttttgggaca ttgttgattc
      481 taattttaac gaggagatga agaatgccca ttctcgacgg ccgggagcaa gaacgaaagg
      541 gtgtcattga aaccgctaat ctgatggttg tttccgctag gacggcgcct aagagcggag
      601 gagtggacga tatcctcacc gcgatcgttt atggagcaga ggtgggagcg ctggccgcgg
      661 acatggagaa attggcggta gagcgaaaaa tagaacgttg gagtaagcag gcccaggaaa
      721 tcaagggctc ggaggtcatc gttctgatcg gggtaagggg gaccaagaga tacgtcactg
      781 actgcggggc ctgcggttac aaatcctgcg acgactttga gaaagcggag aaaaagctcg
      841 gacaggattt tgagggtccc acctgtattt tcaaggccct agatcttggg atcgcggtat
      901 gctcagccgt gaagaccgcc agcctgctga acgtcgataa ccgcattttc taccggatcg
      961 gcgctgccgc caaacggctg aattatctcc cggaagcgag cctaatcatg gggattcctc
     1021 tttcttctga gggcaagaac ccatatttca gccggtaacc atcaatccgt aaagcaaccc
     1081 ttgactgtag aacggtattg taggaaccgc cgcgtccgga ttagcctcaa aaccgcctga
     1141 caagcctcgc ccgatcgttg aaagcctccc tctccacttt caacccgaga atccccgctc
     1201 aaacggcccc aaacaaacct aaggtcttgg ggtcattgac acacaatccc cttttctgta
     1261 ttttcaaccc actccagagc aagtttttat caattcaata gttcaatgcg ctacggatac
     1321 accatgaaca tcgcgttggg ggctgtcgtc ctggccgcgt ttagccgtgc gcggaggcgc
     1381 gggcaggtgg tggcatgccg ttgacgttcc ggcggagggt cactgaaaaa ttgtgggcat
     1441 tcagcgggga aattcccttc cgtccagaga gcggtcagaa atatccctca cttcccggat
     1501 gagcttggaa gcactgccgg gggtgggggg agcgacgcgc agtctttttg tggtcttctt
     1561 cacgggtcgg tgaaaatggg tttccagcct tgtttgctac cccccaaact ggatgggttt
     1621 aaagatcagc ccgggcagcc aggtacagag gtcgggcatg aagatcatga aaaaaaggaa
     1681 ggccagcatg gttaaaaaga atggcaacac ccctcttaga acctggtcca ccgaaatctt
     1741 gccgattccg gatacggcgt aaagattaaa acccaccggc ggggtaatcg ttcccaattc
     1801 cacattgatg gtcatcaata caccaaaatg cagggggtcc acccccaggg ccttggcaat
     1861 aggaaagacc acaggaatgg taagcaggat cattcccatt ccgtccatga aacagcccag
     1921 aatcaggata aaaatcatgt acccgaagag gaaggtcagg ggggagagtc ccatggccgc
     1981 aacagcctcg gcgatcttct ggggccagta ttcattggcc gccaccgcct gaaaaatccc
     2041 cacgctgccc aggagaaaaa aaactacaga ggtgaggcgt acggtgagca ccgttgaggg
     2101 aatgagctcc tttaagaaag ccttcctccg ggcaagggtg ccgtaaagga gggcgtatcc
     2161 gcaagccgca gcgccagcct ccgtgggggt aaagaatccc ccatacaatc ctcccagcac
     2221 gatgaccgga aatgccaatc ccgggaggca acgccagaga ctcaccatgg ccttcccggg
     2281 atcgaagggc ccagtgggta atttttgctt tattccgatc acgaccgtta cggccattaa
     2341 acatccggcc agaatgaacc cggggatgaa tccggcgaag aagagcttgg ccacggaggt
     2401 gtccgtgacc acgccgaaca taagaaggga taggctggga gggatgagca ggcccagcgt
     2461 gccacccgag cacacgatcc cggcggcgag tttgggggag taaccccgct tgatcatctc
     2521 gggaacggcg atcacggtca tcgcggaagc ttccgccacg ctggaaccgg tgatggctgc
     2581 aaagatgaca cagcaaccca gggtggccac cccaaatcct cccggaaccc agctcagaag
     2641 ggcttcggac agatcgaata gcaatttgac cactccggtc ttttccatca aatacccggc
     2701 gtagatgaaa aagggcacgg ccatgatggt gtaatggtgc atgaaataaa ggaaggcgga
     2761 ggggacgaag acggggtcca tggaggtata gaaaatgaca aaaatgacgc tggacgcccc
     2821 cagggccacc ccgatcggag ctccgatgat catcaggagg aaaaaactga taagcagggc
     2881 ttccattcac tctccttttc ccaggctgga aatcgaaaag agacagcgga tcttcttggg
     2941 ccactcggcc cagggatgcc tctcaatcgg ttgaatgagc cgaagccgct tcctcgacaa
     3001 ggacctgctt gcccctcatg gcctggacct cacggaaaat ctcgaacacg aaggaaatga
     3061 tcatcaagcc cattcctata gtcaggatga ggaaaaacgg ccagaagacc atgctctggc
     3121 tcaataccag cctacccgat gaataggtcg tctgggttat tttcacgccg taggtggtga
     3181 aataccccag gtaaaggacg gaggccaact gggccaataa cctcatagca tgacccattc
     3241 ccggatggac cttcttggaa aaaaggtttg taaaaagggt caatttcagg tgcatgccct
     3301 gtcgctcggt gatggagaag aaaagatagg tggccgagag aataaaatag gtcaccacat
     3361 cctgttgcca gtcccaggac tccccgaaaa aataccggcg gatgacctcg acgcaggcca
     3421 gaaggatcgg cccgaggaat acgacagcgg ttatcggagc caggaagcga tcctgaaacc
     3481 atttccagta ctgataagcc ttttccatga gtcttccttt cgatacctcc ggcggagcga
     3541 aaaaaaccac ccaaagggac ggagaaggat aatccgacga tcgccttctc cgtcattgga
     3601 agtgaatgcc ctgcacatcc tttccagact gctcaaggaa ccgcaagtgg gaaggaccgt
     3661 atcttttcct tcgccgggaa cgctacttct ttttctcttc ctggtaggta accaatttct
     3721 tgtaaggagc gcaatccatc gattcgacgg ggtcgcttcc gatcgggtat ttggcgacca
     3781 atttgttccc ctcttcctga aatttgaaga tcagagattt cgattcagcc cccagttttt
     3841 cgatcaacgc ttcggcaact ttggtaccca gggcctgttt aaagggctcc acctgttccg
     3901 gctttagatt gtacatgccg ggtttcttgg ggtccttggt gccgaaatct tcagccacca
     3961 aggtgtcgga gcaccactga agcttttgct ggtagagaat catttcgttc agggtatctt
     4021 tgattatttt ctggtgttcc gaagcaacgc cgtcccacca cttcttgctg aatccgagga
     4081 aatagaagtc cagggccatg cctcccacgc tgatgatcgt gtaataggaa gcctgttcct
     4141 taattaccgc gaaaccgttc gtggtggtaa gcgccccatc gatgactccc gactggatgg
     4201 cgctgggcac gtctccccag gccatggcca cggcgctggc cccccaggct tttaaggtgg
     4261 gaccctgggt gaggggggcg aggaccctga gcttaagtcc cttaaaatcg gcggggacga
     4321 agatcctctt tttggaggcg actcccatgt aatggctgat atcggaccag cccatcacga
     4381 gaatgccctt tttagaaagg cgttcgctaa gggctttggc cacgtcggtt tccgggaaat
     4441 gatgaatggc gccgatggaa gtgagaacca tgggctgggt ggcaatattg agccagggat
     4501 caaaccctgc aaccttgctg ggctggccga cagcgatctc caggttggcg gcctgcaggg
     4561 ctcccatggc atcgctcatg gcgtatagcg ccccggcgaa ataggtgcgt acctcgcttc
     4621 ccgggatacg ctccttgatc ttatcgcgga accagttcat cgaaatggca atcggctggg
     4681 ggggcgggga agtatcggaa acgattcgga tgaccatttt tttcccggtc tgggcggaga
     4741 cagggcttag aaaaccgacc aggaagacca aagccagaga aatgatagaa atccgtttag
     4801 tcatagtcat gcaaaatccc tccttttcaa agaaagattc agaaatctgg gccattcctc
     4861 cttcccgatg ttctgaaaaa gctggggaga atagggtgaa cagcagaaag atatagtatt
     4921 atcggaagcc tgtcaattgt ttcgtggccg agtgtttcgt ggccgagctg aaaattggag
     4981 aaatgttaga atccatatgt atatgtcgtc aggcccaaat ttcttttagg ctgagtttgc
     5041 gatctgactc tggtccgggg tcttgctttg aagaagaccc tcttattaag gagggaggga
     5101 cgaggaaggc taaatctcct gtcagcaggg gggcgtgcta tcaatctacc gctgtaactt
     5161 atttattcat ttggcggcca ggaaagaata ggtcctccct tatcatctgc cttgaatggg
     5221 aaggcgaaag tcctttataa tggcttagta tggaaaacaa tggagctctg accgatagga
     5281 tcggaaaaag gaggaaacat gggggaacaa gtcctggagg gctatatggg aaaggttctg
     5341 cgggtggatt tgacccgggg ggtgatcaag gaagaaccgt tggagaagga gctggtggag
     5401 agttatgtcg ggggcacggg tttggggact gagtatctgt accgagaggt tcctcctggg
     5461 gtaagctggg atgctccgga gaatcgcatt attatggcct cgggtccttt ggggggaacg
     5521 acggtgtcgg ggacgggaac tttttctttg gtgaccaagg ggccgatgac gaacttagcc
     5581 gtttcgacgc aggccaatgg cttttgggga gcgtatctca aattcgcggg gtatgacgca
     5641 gtaattatcc agggaagagc ggatcgttgg aagtatcttt atattggcga gggaagggtg
     5701 gagttaaggg atgcctcggc cctggtgggc aaggacactt gggagacaga agaaatcctc
     5761 cgtcgggagc ttggggcagg ccagaggctg agtgtatttg ggatcgggcc tgcgggggaa
     5821 aaactggtcc gttttgcggt tgtggcgggg gatcaggggc acattgcctc gaagaatggg
     5881 agcggttgcg tgatgggttc taagcgactg aaggccatcg cgatttcccg ggagcagggt
     5941 tctgttccga tatccaacaa gagtcttttg gcggagaagg taaaggcatt ggatttggat
     6001 gcgaaggaaa atcggggagg ccagatgtac aaatggggaa ctggaggagg tttttccggc
     6061 catgccctgg ccgggagtct cccgattcgc aattacacga caaatgtttt tcctgaacat
     6121 gagaagatga gcggacagta tatccgaagt cacttcgagt accggaacaa gccttgttgg
     6181 gcttgcaggc ttgtgcatac gaaatacatg aaggttacgg agggggcata tgccggcgct
     6241 gagggagaag aaccggagta tgaatgcctg gccgcttggg ggcccgtgat cgggaataca
     6301 gaccccggtg cggtgattgt gctttccgat ttgacggatc ggttggggat ggacgtaaac
     6361 gaggcgggtt ggaccattgg ttgggtgatg gaatgttatg aaaaagggct cctcagccgc
     6421 ggagacctgg atggcttgga aatgagatgg gggaatgtgg aggctgtccg ggagatgttg
     6481 aaaaagatca gtcagcggga ggggattgga gaccttttag cggaaggggt gaagcgggca
     6541 tcggaaaaga taggtggcga ggcactcaag atgggggtat acgttcagaa gggtgcgaca
     6601 cccagagggc atgatcaccg ggccatatgg gcggagatgt tggatacttg tgtttcagcc
     6661 actggcacca tccagtctgt atcgcgcatg gttccgcttc ccgtttttgg tcatccgccg
     6721 gtgagcaatc ccttttctcc gtgggaggtg gcaggggcga gtgcgaaagc agagggttgg
     6781 tatgtgttta tggattgtct ggggatttgc cggtttacag ccattgatcc gaagttgacc
     6841 ctggattgtg tgaacgcggt gacgggaaga ggactgacct tctcggatgc cctgatcatc
     6901 gggcggcgga ttataaatca gttgcgggta tttaatttcc ggcatgggtt ggaccctgct
     6961 ctggagattc cttcagctcg ttatgggtct gcaccagtgg atgggcctgc gaagggaaaa
     7021 gccatcgggc ctcattttga gtggatgaag aggttttatt ttgagctgat gggatgggat
     7081 ccggagacgg gcaagccgtt accgcacacg ctcaagtcgc tgggtctgga aaagctcatt
     7141 gaggcatttt gaggatcgaa tcggcacgag ttcctatgat gaatgggaag atcagattac
     7201 agttttggat ggcgggtgcc cccgagggga cccaatcggg cccgatgatg atggaggagg
     7261 agatccaaga ggatgagtct ttgcgcagcc tattgaatcg ccttgcggga cgactccctc
     7321 agcttcctgt ccccatcttt gatcccgaaa cgcagacctt gtcgagcgaa gtggcggtgg
     7381 taatcaatga tcatctccat catttttccc aaggactgga gacgaaactc aaggacggag
     7441 atcgcattct cattttcccc tatttggctg ggggataggc aagagaagat taaccctctt
     7501 ctttttgggg aacgattttc ctttaggacc ttccatgaag aaaccttgca ggttgaaagc
     7561 gtaaccgagc acatcgaaca tttccctctt cttgaaatcg attacgtctt ttatcgcctg
     7621 cttctggact ggagaaagat cggaaggcca cgcagaattt tcatgtcctt cgactcatca
     7681 cccctttgcg ggtgagctgt gagaaatcct gtgcgaaggc cttcccgttt gacaaaatgg
     7741 tcagcgacat gctgaacccg gaaatgatcg agggcacggc caaaagccca aacgaggcta
     7801 ttaaggacaa gttccggagc aacataacaa aaacgaagag aggcaaagag aggggaagaa
     7861 cgataaatta aatttgaagt taaatttgaa ttgaccgatt aaggggataa tgctaatttt
     7921 aaactttgaa ataacgggtg gtgagattga aaagccgggt tttgattatc tctctcattt
     7981 ttgctatttg ctttccttct ttggccgcct tcggcgcaga gtgggccgac ttccaggatt
     8041 ttttgatccg aagaattccc ttccgcccct attgggcaat ggccgggtcc gagtttgcca
     8101 aatatatc
//
LOCUS       DASPRR010000096         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2283614, whole genome shotgun sequence.
ACCESSION   DASPRR010000096 DASPRR010000000
VERSION     DASPRR010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283614"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VEL68_03410"
     CDS             complement(<1..1351)
                     /locus_tag="VEL68_03410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ35047.1"
                     /translation="MAEITILKEISLFLPDHSTRFGSIAFDSSGIIAVGDVERVRGKA
                     GKMARSISLPGALVIPGFVDGHTHMLAGAQQLTRIDLRTVKSRDEFSRNLQTWVKENP
                     SNLWILGQWSDTLLGGELPSRSWVDGASQGKPLFLARFDMHSAVANSRALELAGIDKK
                     TPDPSGGRIVRDPRTGEPTGLLLEKAMDLVADKIPPLTDSQKAQWMENAIQYAHGFGL
                     TAVHDVFFHSKWEDLNIYRQVLQREKTGLRIFLRVSLRDLTRLLSALKEPLPDRLILD
                     GVKGFIDGTLGSTTAWLEEPYVHNPSESGIPCIDDPEEFRQGVRSAAAQGVTVSLHAI
                     GDAAIGFAQEIYRECIQQGLGQAPLRIEHFQHPGEKHIASMNHPRLFASVQPAHLLDD
                     ASVAEERLGPRRAALSYPIRSLLDKGCRVIFGSDWDVADLNPLVGLQAAVTRVDKANR
                     "
     gene            complement(1523..2701)
                     /locus_tag="VEL68_03415"
     CDS             complement(1523..2701)
                     /locus_tag="VEL68_03415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXZ35048.1"
                     /translation="MNRFAASRMQRIEQSGIRRMLEKAMAMERAGGGVIHMEIGRTDF
                     DTPAVVKEAAKKALDEGWVHYTSSMGMLELRQAIAEKLRRENGIEADPEKEILAVAGA
                     SHGMAITLMTVLEAGEEIIIPEPMYLFYLDWAEYLGARTVPLPCFPEDGFQISRERLE
                     ACVTGRTKAILLNSPHNPTGSGLELSSLKAIAEVAIERDLLIISDEIYEKIIYEPFHH
                     ISIASFPGMKERTVTLNSFSKGHAMDGWRIGYLAAPADLVWELEKGQQHTMINPTTFS
                     QIGAITALKMGDSIIRPMVEEYTRRRKLMMEMADRAGLSYFPPQGAFYFWLRHGVTKV
                     DGWALVDYLLDKYGVAITPGEIFGPSGKGYLRISYSNSTENLRKGMARIIQTLEELRS
                     "
BASE COUNT          664 a          836 c          834 g          691 t
ORIGIN      
        1 aacgattggc tttgtccact cgggtcactg ccgcttgaag acctaccagg ggattgaggt
       61 cggctacgtc ccagtccgag ccaaagatca cccggcaacc tttatccaac aaagacctga
      121 tgggataaga gagtgccgcc cggcggggcc ccagtctttc ctccgccacc gaggcatcat
      181 ccaagagatg agccggctgg acagaagcga agagacgggg atgattcatg gaggcgatat
      241 gcttttcccc cgggtgttga aaatgctcga tccgcagggg ggcttggccc agcccttgct
      301 gaatgcactc tcggtagatt tcctgagcga agccgatggc cgcgtcgccg atggcgtgga
      361 gggacaccgt aactccctgc gcggcagcgc ttctcacccc ttgccggaat tcttcggggt
      421 cgtcgatgca ggggattccg ctctccgagg gattgtggac ataaggttct tccagccagg
      481 ccgtagtgga gccgagagtg ccatcgatga atcccttcac tccatccaag atgagccgat
      541 ccggaagtgg ttccttcagg gctgaaagaa ggcgggttag atcgcggagt gacacccgca
      601 aaaagatccg cagcccggtt ttctctcgct gcagaacctg ccggtaaata ttcaggtctt
      661 cccatttgga atggaaaaac acatcatgga ccgcagtgag cccgaaaccg tgagcatatt
      721 ggatggcatt ctccatccat tgcgctttct gggaatcggt caggggggga atcttatcgg
      781 ccaccaggtc catggccttt tccagcagca agccggttgg ctcgcctgtc cggggatcac
      841 ggacaatcct ccccccagaa ggatcggggg ttttcttgtc aatcccggcc aattccagag
      901 cccgagagtt ggccacagcg ctgtgcatat cgaatcgggc cagaaaaagg ggtttcccct
      961 gggaggcacc atccacccaa ctcctgcttg ggagctctcc gccgagcaga gtgtcagacc
     1021 actggccaag gatccagaga ttcgaggggt tttctttcac ccaggtttgg aggttgcggg
     1081 aaaactcatc ccggcttttg accgtccgca gatcgattcg ggtaagctgc tgggctccgg
     1141 caagcatgtg cgtgtggccg tccacaaatc ccggaatcac caaggcgccg gggagggaaa
     1201 tcgacctggc cattttaccg gcctttcccc gaactctttc cacatcaccc acggcgatga
     1261 ttccggaaga gtcgaaggct atcgacccaa accgtgtgga atgatcaggt aagaatagag
     1321 aaatctcctt caaaatggtt atctctgcca aggcacaatt cctttatggc caattccagg
     1381 gagtccattc aaggccccct tgggggaatg atgtcagggc aattcatgaa ttgccgattt
     1441 ccttgtcctg agcaggagcg ggtttaaaac ccgcccccac atcgtgcgat gacccaacaa
     1501 cggaatttaa aggggtaccc tatcacgacc gcagttcttc cagggtctga atgattcgag
     1561 ccatcccttt ccgcaggttc tccgtggagt tggaatagga aatccgcaga tacccttttc
     1621 cggaaggacc gaaaatctct ccaggagtga tggccacccc gtacttatcc aggaggtagt
     1681 caaccaatgc ccagccgtcg accttcgtga ctccgtggcg gagccagaaa taaaaagccc
     1741 cctgcggagg aaaataggaa agcccggccc gatcggccat ttccatcatc aactttctcc
     1801 tgcgggtgta ttcttccacc atgggccgaa tgatggaatc ccccattttc agcgcggtga
     1861 tggccccgat ctggctgaag gtcgtcgggt tgatcatggt atgctgctga cctttttcca
     1921 actcccaaac caggtcggct ggcgccgcca ggtagccaat cctccagccg tccatggcgt
     1981 gccctttgga gaaggaattc agagtgacgg tccgttcctt cattcccgga aaagaggcaa
     2041 tgctgatatg atggaaaggc tcgtaaataa tcttttcgta aatttcgtcg gaaatgatta
     2101 ggaggtccct ctcaatggct acctcggcaa tagctttcag gctggataat tccaggccgg
     2161 agccggtggg gttgtgcggg ctgttgagga gaatggcctt ggttctgccc gtcacgcagg
     2221 cttccagccg ttctctggaa atctgaaagc cgtcttcggg gaaacagggg agggggacag
     2281 tcctggctcc caggtattcg gcccagtcca ggtagaaaag gtacatgggc tcggggatga
     2341 tgatctcttc tcccgcctcc agcacggtca tgagagtaat ggccatgccg tgggaggctc
     2401 cggccacggc caggatttct ttctccgggt cagcctcgat cccgttttcc cggcgaagtt
     2461 tttcggcgat ggcctgcctg agctccagca tgcccatgga cgaggtataa tgcacccatc
     2521 cttcgtccag ggctttcttg gccgcttctt tgaccaccgc gggggtatca aagtctgtcc
     2581 ggccgatctc catgtggatg actccgccac cggccctttc catggccatg gctttttcca
     2641 gcatgcggcg gatgccggac tgctcgattc tttgcattcg ggaggcggca aatcggttca
     2701 ttttttctct ataagggacg cagatgagcg cagattttca ggataaaaca taaactatag
     2761 tgaacgtcaa aagaaagttg actttgtatg cccgcattcc cctgcaaaat tcccccaacc
     2821 cccctttatc aaaggggggc aaggggggat ttaatcggcg gattattaaa ggccgctgtc
     2881 tggccgccca acgtcaactt tttaagtcgg tgactataaa aataatctgg gctcatgatt
     2941 caacaaatat ctatctcgat tatctgcgcc ctgatttttt tattgggata gcatagggtt
     3001 tgaagagagt caactgcttt gtcga
//
LOCUS       DASPRR010000097         4655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_509007, whole genome shotgun sequence.
ACCESSION   DASPRR010000097 DASPRR010000000
VERSION     DASPRR010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509007"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1612)
                     /locus_tag="VEL68_03420"
     CDS             complement(209..1612)
                     /locus_tag="VEL68_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXZ35049.1"
                     /translation="MKRYGPYIRVLLAGFLVVCLNAQAGQPLLAQSSPSPPAGEKSED
                     LTLSAAVELALQSNPRVKAVLSSREMTDAQVREARSGWLPLLQFSETFTRSNNPVFVF
                     GSLLEQGRFGPQNFAVDSLNNPDSLNNFRTAINLKWSVFDQMQSSAQVAQARIGQQQA
                     DHQKEMVKQQVRLEVVRAYFGVLLAQQRKDVTEEAVKMGEADRKRIADLFQSGLVVQS
                     DLLSAEVQLAEFRQQQVQASGDLVTAHAFLNTVLGTPVQTLQRIAGQLLDKQFFMAET
                     DELIRQALQSRPDYARAGSAVKSSEEGVRGAKGNYLPRIDVFSSYGLSGRDLTSGSSD
                     YAVGAGLTLNLFDFGRGARLDKARAAQSTAAAEQEHLANQIRLEVIQVYQDYRSAGER
                     LKVAGQTVTQAQEALRIVKDRYQEGLTTITEVLRAQTAYLRTRLNLISSRYDHYIGYA
                     RVLMATGKLTDVTPFNS"
     gene            complement(1815..2207)
                     /locus_tag="VEL68_03425"
     CDS             complement(1815..2207)
                     /locus_tag="VEL68_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ35050.1"
                     /translation="MMKPAKDSLDLGILVSDIKASLNFYQNILGLEFVGITPVWFGTM
                     HRLRFGTSDFKLVDPKTVPPKGVIGLEKQLGFRYVTFVIKNLTELCAELKGKGIEFSL
                     PETEVRPGIRVAMVKDPDGNIVEFVERR"
     gene            2382..2957
                     /locus_tag="VEL68_03430"
     CDS             2382..2957
                     /locus_tag="VEL68_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HXZ35051.1"
                     /translation="MKSNIFDKTCKIGFDRQEEIPLQFSLGIPYASLDALPIPFRFPP
                     KENKKLPELSRFLGILIRMFAEFNAPHHVTHSCAYYQEDSPVFRIDAIDIISGVLPKK
                     QQPFVEAWAELHQGELWAGKNAHLSASWLWLESSFFRQMFLKGSRWANQLSLMFQHSC
                     SFRQGWTKVRIRYCRRRACGKTAPKFLHAFF"
     gene            complement(3163..>4655)
                     /locus_tag="VEL68_03435"
     CDS             complement(3163..>4655)
                     /locus_tag="VEL68_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXZ35052.1"
                     /translation="LIAAYYSVYVYGSNGALLSQADAGSGRVYAPHVVVDLEGDGIVE
                     IVFGKGSQVFAYEWRNKALVLKPGWPVDTTTAGESPEVRGLAAADLDGDGVIEIIATT
                     TQTQSTANGGAQVFVFSSNGRLFQPAGLSYPAWPRYNNRSGEGGDADRNGPGHSGYGC
                     YGLNVGVGNIDDDPNLEIIVTYDNHLIQAFDLNGVAINASPWFTNRTSPYVGNRMTWG
                     QFIRWADPQVEENHYHLHVGDWPDPTRQEWLQWTASPPNIVDLDGDGKNEVLGVPNVE
                     KNEPYQTQAYAIMALEGSHGDGSRSAMRKTGWETLPRGAAPIQVSGGYPPMGIPAATT
                     VNLQGDSRPEIIVSLNDGFIRAYSADAKEIWRFNYTHGKAVMYASEATVADLNQDGSP
                     EVIFTTYGDPNVLDSGYLVILGADGRLLHDIPLPNPGRNGNGNGAPAAPAIYDLDGDG
                     QLEIFVQTFDHGMDIFTVPGSASNCVLWPTARGGPLRMGQPNSNGL"
BASE COUNT         1021 a         1302 c         1231 g         1101 t
ORIGIN      
        1 ctttctcttc cttcttgccg caggaagaag agccggccag aagcgcaaca atgcagatga
       61 ggacaatttt ctttttcatt tgcatcctca aatgatcggg aggtagatta acgcagacca
      121 gcgcagagaa aaaataagag ccaaaaccaa gtcctttcat ttattctata tcccggaaac
      181 ccgcgttcct ctgcctccca ttgggaattt atgaattgaa gggggtcacg tcggtcagct
      241 ttccagtggc catcagcact cgggcatatc caatataatg atcatacctt gacgaaatca
      301 gattcaatcg agtcctaagg taagccgtct gcgcccggag gacctcggtg atggtcgtca
      361 acccttcttg gtaacggtcc ttgacaatgc gcaaggcttc ctgggcctgg gttacggtct
      421 gccctgccac cttcaaccgc tccccggcgg acctataatc ctggtagacc tggatcacct
      481 ccagacggat ctggttggcc agatgttcct gttccgcagc cgccgtggat tgagctgccc
      541 gggccttgtc caggcgggcg ccccgaccga aatcgaacaa gttaagagtc agtccggctc
      601 cgacggcgta atccgaactg ccgctggtca ggtctcttcc gctgagtcca tagcttgaaa
      661 agacatcgat ccttggaaga taattcccct tggctccccg gactccttcc tcggacgact
      721 tgaccgcaga gccggcccgg gcgtaatccg gcctgctctg cagcgcctgg cggatcagtt
      781 catcggtttc cgccataaaa aattgtttgt cgagcaattg accggcgatc ctttgaaggg
      841 tctggaccgg agtccccagg accgtgttca aaaaggcatg ggccgtaacc aggtctcctg
      901 aagcttggac ctgctgctgc cggaattcgg ccagctgaac ctctgccgaa aggaggtccg
      961 actggaccac cagcccggat tggaaaaggt cggcaatccg tttccgatcc gcctcgccca
     1021 tctttaccgc ctcctcggtc acatccttcc gctgctgagc caggaggacc ccaaagtagg
     1081 cccggacgac ttccaagcgg acctgctgct tcaccatctc cttctgatgg tctgcctgtt
     1141 gctgaccgat gcgagcctgg gccacctgcg cgctggactg catctggtcg aaaacggacc
     1201 atttcagatt gatggcggtg cggaaattgt ttagcgagtc cgggttattc agcgaatcga
     1261 cggcaaagtt ctgtggcccg aaccggccct gctccaagag ggagccgaag acgaagaccg
     1321 ggttgttgct ccgggtgaag gtttcgctga actgcaggag cggtaaccac ccggaccggg
     1381 cctcccggac ttgagcatcc gtcatttccc tgcttgaaag aacggcctta acccgagggt
     1441 tgctttgcaa ggccaattcg accgccgccg ataaagttaa gtcttctgat ttttccccgg
     1501 cgggaggcga gggggatgac tgggcaagaa gcggttgccc ggcttgagca ttcaggcaga
     1561 cgaccagaaa accagctagc agaactctga tataggggcc ataccgtttc atgatccatc
     1621 ctcctgagga tttcctgaag ggagctaata accaaccaaa tccggcaaga ctcgtccgga
     1681 tcccgggttt tttctaagaa tgaaattcaa gggttaaata agggggaaaa aatgtattga
     1741 atggaggttg ctaaatcttt tgtggcctgc ggggggaaga gaatccttcc ccccatcttt
     1801 ttattactat ccgcttagcg ccgttccaca aactcgacga tgtttccatc cgggtctttt
     1861 accatggcga cccgtattcc cgggcgaacc tcggtctccg gcagcgaaaa ctcgattccc
     1921 ttgcccttga gctcggcgca gagctcggta aggttcttga tgacaaaggt tacgtagcga
     1981 aaacccagct gtttctccaa accgatgact cccttgggag gaacggtctt ggggtcgacc
     2041 aatttgaaat cgctggttcc gaatcgcagc cgatgcatgg tcccaaacca aacgggggta
     2101 attcccacaa actccagccc cagaatattc tgataaaaat tgaggctggc cttgatgtcg
     2161 ctgaccagga tccccaggtc cagagagtct ttcgccggtt tcatcatgat ccctttctcc
     2221 tttcgcaatg gactttcatt ttctgtagcc cttttgtctt tttccctgaa tcaccctagc
     2281 gcatagattt aatgtactaa atttttgagg ggatcgtcaa tcgtgcttta acatgcccat
     2341 ccttcgctcc gatcttcttt ccggcctatc ttcctcgaag attgaaatct aatatttttg
     2401 ataaaacctg taaaattgga ttcgacagac aggaagaaat tccccttcaa ttttcactgg
     2461 gcattccata cgcttctctt gacgctttgc ctatcccttt tcgcttccct ccaaaggaaa
     2521 ataagaaatt gccggaactg agccgctttt tgggaatcct tattcggatg tttgcggaat
     2581 tcaatgcgcc gcatcacgtc actcattcct gtgcctatta tcaggaggat tctccggttt
     2641 tcagaattga cgctattgat ataatttcag gggttttgcc caaaaaacag cagccctttg
     2701 tggaagcttg ggctgagctg caccagggtg agctatgggc gggaaagaac gcgcatctta
     2761 gtgcaagctg gctttggctt gaatcttctt tttttaggca aatgtttctg aaaggttcac
     2821 gatgggcaaa tcagctatca ttaatgtttc aacactcctg cagctttaga cagggttgga
     2881 ctaaggtaag aattcgatat tgcaggcgaa gggcatgcgg aaagactgct ccgaagttct
     2941 tgcacgcttt tttttgaacc taagatcatt cttcaaagaa aacaaatatt ctaagagcta
     3001 accgccgcca cctccttgga atccccgctc cgtggagatg gatgaaacca tagcgaggat
     3061 gaaaccgcta tcggaaaata aatcatccaa tgaaaacaac cccttcccta aaagatcttc
     3121 aaccgaaaca tctcgaaggg agtacggatt gcccggcgaa gctcagagtc cgttgctgtt
     3181 tggctgcccc attcgcagag ggccgccacg ggctgtcggc caaagcacac agttggaggc
     3241 tgatcccggg acggtgaaga tatccatgcc atggtcaaag gtttggacaa aaatttccag
     3301 ctggccatct ccgtcaagat cataaatcgc tggagcggcc ggagcaccgt tgccgtttcc
     3361 gtttcggcca ggattgggaa gagggatgtc atgcagcagc ctgccgtccg cacccaatat
     3421 caccaaatat ccggagtcga gaacgttggg gtccccgtag gtcgtgaaga tgacctccgg
     3481 ggagccatcc tggttgaggt ccgcgacggt cgcctcggaa gcatacatta ctgcctttcc
     3541 atgggtgtaa ttgaatcgcc agatttcctt cgcatcggcg ctgtaggccc ggataaaccc
     3601 gtcgttcagg gagacgatga tctccgggcg gctgtcgcct tgaagattga ccgtggtcgc
     3661 tgcgggaatg cccatgggtg ggtacccccc gcttacctgg atcggggccg ccccccgggg
     3721 cagggtctcc cagccggttt tgcgcatggc ggagcggctg ccatccccgt gggaaccttc
     3781 cagtgccatg atcgcataag cctgagtctg gtagggctca tttttctcca cgttcgggac
     3841 cccgagcact tcgttcttgc cgtcgccatc caggtcgacg atgttcggag gggaggcggt
     3901 ccactgcagc cattcctgcc gggttgggtc gggccagtcg ccgacgtgca agtgatagtg
     3961 gttttcctca acttgcggat cggcccagcg aatgaactgc ccccaggtca tgcggttgcc
     4021 tacatacgga cttgtgcggt tggtaaacca cggggaagcg ttgatggcca cgccatttag
     4081 gtcgaacgcc tggatcagat ggttgtcgta ggtgacgatg atctcaagat tcgggtcgtc
     4141 gtcgatattt cccacaccca cattaagtcc ataacagccg tagccggaat gacccgggcc
     4201 gttgcgatcg gcatcgccgc cttcgccgct tcggttattg tatcgcggcc aagccgggta
     4261 gctcaatcca gcgggttgga agaggcggcc attgctggaa aacacgaata cctgcgcgcc
     4321 gccatttgcg gtgctctggg tttgggtggt ggtggcgatg atttcaatca cgccgtctcc
     4381 atcgagatca gcggcagcca gcccccggac ttccggcgat tcccctgccg tggtcgtatc
     4441 gaccggccat cccggcttca ggaccagcgc tttgtttctc cactcgtagg caaagacctg
     4501 ggaccctttt ccgaaaacga tttccacaat gccatccccc tccagatcga ccaccacgtg
     4561 gggggcgtat acgcggccac tgcccgcgtc cgcctgagag agcagggccc cgttgctccc
     4621 gtagacatag actgaataat aagccgcaat gagct
//
LOCUS       DASPRR010000098        26935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_990303, whole genome shotgun sequence.
ACCESSION   DASPRR010000098 DASPRR010000000
VERSION     DASPRR010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 26935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26935)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26935
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990303"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..691)
                     /locus_tag="VEL68_03440"
     CDS             complement(272..691)
                     /locus_tag="VEL68_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HXZ35053.1"
                     /translation="MENSKVHITVFYCSQQGEKVHQILTGLDGSGGGKFRRIPLPCSG
                     KMEIILLTKALESGADGVALFGCPEGACRYLVGSSRAKGRVAHVGKILEEIGLEKDRL
                     QRFVLGNQGPDENVREMGKWVEKIRSLGAIGPLQNGK"
     gene            complement(827..2155)
                     /locus_tag="VEL68_03445"
     CDS             complement(827..2155)
                     /locus_tag="VEL68_03445"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HXZ35054.1"
                     /translation="MAKETAAKIMKKMEENDVKFVKIWFTDILGFIKSFSVPIEEMEK
                     AFDEGIGFDGSSIEGFVRIDESDMVAIPDPSTFAILPWRPKEKGVARVFCDIVTPDGK
                     PFEGDPRFVLKKNLEKAKKMGFTYYTGPELEYFYFKDSHSTEVLDRGGYFDLIPRDEA
                     IDLRRETVLFCEALGIKIEYSHHEVAPSQHEIDMRYEDALTMADNVMTYRLVVKEVAY
                     RQGVYATFMPKPLFGENGSGMHTHMSLFKGNRNAFFDAKGQYHLSDTARYFIGGLLKY
                     APELTSITCQWVNSYKRLVPGYEAPVYLSWAQRNRSDLVRVPMYKPGKEEATRIEFRS
                     PDPACNPYLAFSVMLAAGLAGIEQKVIPPDPVEENVYKMSEEERLKRRIRQLPGSLLE
                     AIQLTEKSQIVREALGDHVFEHFIENKKVEWDRYRIQITSYEIDKYLPIL"
     gene            complement(2177..2566)
                     /locus_tag="VEL68_03450"
     CDS             complement(2177..2566)
                     /locus_tag="VEL68_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXZ35055.1"
                     /translation="MAAILVIKDDHELQGLIERILPPGEARMTGNNLPEPLQRLLPTK
                     EQKPEEPTQTLEGVVREVVKSEVRTGNDDHIHESVIGKVERALIGMLLEEERGNQVRA
                     ARRLGINRNTLRKKMKDLQIITRVITS"
     gene            complement(2876..5515)
                     /gene="glnD"
                     /locus_tag="VEL68_03455"
     CDS             complement(2876..5515)
                     /gene="glnD"
                     /locus_tag="VEL68_03455"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXZ35056.1"
                     /translation="MSTEPTKDSLEDIERFLRSGQEWLKKRHEEGAAGAEMCQSRSAL
                     MDNVLRRLWKGAFREPGEKETGEGVALLAMGGYGREELNPFSDIDLFFLHLPGKGKNL
                     ESWVKKILHPLWDWGLTVGYTVQTPKESWKTAQKDLDLLLSFLDARWMAGDKGAFLRW
                     EEEFTRAIKGGQEKEVILQIQQSAESRHKKLGDSVFVLEPEIKDGKGGLRDYHAAFWA
                     AKIKFSLRSARQMAEQGLLSEKEWETYSQALDFLWRVRNQLHYLHGRREDRLSFEDQT
                     TLAQVLGYRGEDSLRNTESFLKDYFHQALRVHHLSWNILEKCLNEVQDSTRTWWRKAA
                     PEIATGFHMYHGRLALTDPASLDRNPLQIWNVFENVHRHGIELSVGLQEIIAQKLGLM
                     NDRFRSAPESIRSFLSYFEQPGHLYRVLEEMYETGFLRRFLPEFERVHCHIQYDRYHL
                     YPVDVHSLYAVQELEILERKNGPPPWPLFEQLMREVPDPALLKLAALVHDLGKAEGTP
                     HALRGEKIAESVGERLGLSAERIDTLRFLVREHLTLAEIAQRRDLNDENLIFRFAQTV
                     GNRERLKMLYLLAFADLRAVGPSAWTAWKDTLLRELFLKTLHLLDRGEGLRREDQERV
                     IGVQREIMELVKGQIPPPKIAEYLVNIPRRHYQVHEARAIGKQVLMAEKLADQAVALD
                     GEEKPEEGGDEITVVARDEPGLFAKICGVLTANLLNILSAQISTWENGIAVDQFRVQS
                     LIEEGLLQPRRWTKLQEDLKKVLEGKVEVDRLLQEVVPPLFHRYPTVRSAPQVEVDNV
                     TSDFYTLVEVFTHDRPGLLYRIAQKLFEMGLNLWMARISTKVDQVVDVFYVQDMSGAK
                     IEDEQKISAIKEELLRELERPET"
     gene            complement(5635..5973)
                     /locus_tag="VEL68_03460"
     CDS             complement(5635..5973)
                     /locus_tag="VEL68_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXZ35057.1"
                     /translation="MKKVEAIIKPFKLDEVKNALSSVAVQGMTVTEVRGFGRQKGHKE
                     VYRGAEYIVELVPKIKIEVIVPDGAVTRVVETITKAAKTGKIGDGKIFVLPVEEAVRI
                     RTGERGEGAL"
     gene            complement(6051..7427)
                     /locus_tag="VEL68_03465"
     CDS             complement(6051..7427)
                     /locus_tag="VEL68_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXZ35058.1"
                     /translation="MKKGVLVLGILLWLGPGIGMADDPPALKTLVPATVGTPAAIQPA
                     STVSPAGKINPGDTAWILISAALVMLMTPGLALFYGGMVRTKNVLGTIMQSFMVIALI
                     SVQWALFGYSITFGPTVGGIIGDLSWLGLKGVGLAPNPDYAETVPHQAFMIYQGMFAV
                     ITPALLMGAIAERMKFSAFFIFTLLWSTLVYDPIAHWVWGVGGWIRKMGGLDFAGGIV
                     VHLSSGISALAAAILVGKRKGYGKEPMAPHSLPMTLMGTGILWFGWFGFNAGSAITAG
                     MLSTSAFVATHLAAAAGALSWALVEWAYKGKPTTLGVASGAVAGLATITPASGFVTPL
                     AALFIGLTAGVFCYVGVFAKNKFRYDDSLDVVGIHGVGGTWGALSIGLLATKEINEAG
                     NNGLLWGNPELFGVQAVAVLVTMAYAFAVTFILLKGLGWAMGLRLNEEEENSGLDLTQ
                     HNETGYNY"
     gene            complement(7731..8069)
                     /locus_tag="VEL68_03470"
     CDS             complement(7731..8069)
                     /locus_tag="VEL68_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXZ35059.1"
                     /translation="MKKVEAIVKPFKLDEVKAALSSVAVQGMTVTEVQGFGRQKGHKE
                     VYRGAEYVVELVPKIKIEVIVPDGAVTRVVETITKAAKTGKIGDGKIFVLPVEEAVRI
                     RTGERGEGAL"
     gene            complement(8168..9550)
                     /locus_tag="VEL68_03475"
     CDS             complement(8168..9550)
                     /locus_tag="VEL68_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXZ35060.1"
                     /translation="MKKSFMAIILMMALVVSAGLTFAEEKPKAEGPAPGAAETAKPAA
                     SAPTAPAPPKIDTGDTAWMLASAALVLLMTPGLGLFYGGMVRTKNVLGTIMHSFIIIA
                     LITIQWILWGYSLAFGPDVGGIIGSLSWFGLRGVGLDPYPDYAATIPHQAFMIYQAMF
                     AIITPALITGAIAERMKFKTFIVFTLLWATLVYDPIAHWVWGVGGWIRSMGALDFAGG
                     TVVHISSGISALAAAILVGKRKGFGRDPMGPHNLPMTIMGAAILWFGWFGFNAGSAIA
                     AGKLSTSAFVVTHIATAAAALSWPAAEWIYRGKPTTLGAASGAVAGLVAITPASGFVG
                     PISALIIGLTAGILCYAGVMGKNRFGYDDSLDVVGVHGVGGTWGALATGLFATKAIND
                     AGDNGLFFGNPGLFGVQAVAVLVTWVYAFVVTLVLLKVLDWTLGLRVSEEEEEMGLDL
                     SQHSETGYNF"
     gene            complement(9588..10754)
                     /locus_tag="VEL68_03480"
     CDS             complement(9588..10754)
                     /locus_tag="VEL68_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXZ35061.1"
                     /translation="MKNKLANTCLGVFLVIISAMSAGGQEAKPEAAPAAKAEGKPEAK
                     PVTSLDDIKNLLGMSLYFQGGYTYNFEKPDSGINRQRIFDQKANTFLIDMAQVQFAKD
                     APEGGLGFKLKVSAGETAKYIHSAGLGNPNDEFDLTEAYVDYVAPLGSGLKLRFGKFA
                     TYHSAEVIEARDNFNYSRSFLFNYAVPFTHTGFMAQYALSKEFSASLYLVNGWDVTTD
                     NNNGKSVGASFGFTPAEILSFTFNFMYGPEQANNSSHNRFLFDWVGAIKPSKELTFMA
                     NIDYAREDSDPNNGGNNSEWYGAAVYAKYEFADFFSASIRAEYFDDQNGVRTGIAQKL
                     KEITLSPEFKIAKNLLVRPEYRHDWSDQKGFDSHKNAFDKKSQDTIAFGMMYTW"
     gene            11235..12230
                     /locus_tag="VEL68_03485"
     CDS             11235..12230
                     /locus_tag="VEL68_03485"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ35062.1"
                     /translation="MMDSTPYQEFSKMLGAPNSKVLPRILEKIADEKELKVLMVAFPP
                     ATVSEFSEKTGIPREEVQAMVRTLFQKGLLFSSKKEGEPRYYRVKTVPQFHDSSILWK
                     EATKEFHALWKEYTDKEWADFGKVIDAFLQKPAVRIIPVGATIESKAKVLALEDVREI
                     VKQARILAVTPCTCRVVDGKCGKPVEVCIQVNRAADYAVERGTGRVITREEALHILHQ
                     AEKEGLIHVVDNRQKVDHIICNCCRDCCILQRLPNPHKFLDPSRFQAMVNAEECIGCQ
                     ACLERCMFGAIRMEGDEPKARVFAERCVGCGVCTIGCPTEAISMKEVRPPDVIPV"
     gene            complement(12281..13123)
                     /locus_tag="VEL68_03490"
     CDS             complement(12281..13123)
                     /locus_tag="VEL68_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ35063.1"
                     /translation="MEEEILRRRQLEGELQDYKKKCDLLLQYLNEAIGAIQKLGNQVE
                     RTPRIEMMGKAMVAGLAHDLRNPLAVIQSCSQSCLAKEKLSRSLKKSLKMIQESSQRA
                     NSLLRQFLDFIKSGLNRQPVDIHELLFRAWDAARLDRKCARVVLESRLAADLPLIDAD
                     PEKLERVFINLFLNALGAVASGGKITLQSRFLPSDNRAQIEVIDDGPGIPSSERNRIF
                     EPFFSTRKGGVGLGLFLCKSLIRDHHGEINVDCGDCGGTKVTVNLPVVQEDDQEKGKN
                     PGIE"
     gene            complement(13363..14826)
                     /locus_tag="VEL68_03495"
     CDS             complement(13363..14826)
                     /locus_tag="VEL68_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXZ35064.1"
                     /translation="MAKTDETLEKLLTIEKAVNEMVTLRQGILELKESEAQRQKALEE
                     LQEVGSHYRILLENLPQRIFMKDKNSVYLLCNRAFAANLQKKPEEIAGKTDDDFYPKE
                     LAEKYRTSDQRIMATGCSESIEEVLVREGHSFVWKTIKIPFKNENGNILGIIGVSWDI
                     SEQRRKDEELKLKFTHSEEMVDHLKPELQKLNEKLQKEMIERRRTEEELQGAEEFRRV
                     FENVATPVVLLEGNKIISLANKEFEKFSGYSKEEVEGKKKWPEFSAPSHVEGKERCPG
                     SLDENLEEVPPDGEYRFRDKAGNLREVSCKFGKMPETKRIIVSIFDITHQKRTEEWIG
                     SLEEKYNSLMDNTREALAVVQEGIIRFVNPKGLEVLGYAKDELASMTFSDLILPEDRE
                     RIEILPQTLKTGEPAQPKPFRVIHKDGRTRWLEGRGALIQWGKKPAALYSLTDITDRM
                     QAKEELRTSLEPFRALVNAMDKYLSALNGVETKIQKG"
     gene            complement(14888..16018)
                     /locus_tag="VEL68_03500"
     CDS             complement(14888..16018)
                     /locus_tag="VEL68_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXZ35065.1"
                     /translation="MVFGKLLGKQSKTGGPLRGLDASETLEYIDNFETAIFQVSSALK
                     SYKDPNKILEVISQESINCLRAHRSTIFLLEAKGEALKIKFTHALDPRYQQVGLVEEK
                     EIAKRTLRQGSPLLLSGPESFSRFFKYEERDNKITSLLSIPLLAKGKPMGVISVALIN
                     GSYSFDEKSLRCISSLANFASHAIEIADLLEEVQKGSSVRTAYEGHLDNILNQLQSLS
                     QKEQQRIDTHIIMIKAEQELDDNEFLENKTNGKVPWAQGAIILKDERRKDERVEAKVR
                     VEFDEEHWGVTDNLTLGGAFVLTPNPMDLGDEFLLRIHIPDGREPAEGECKVVWTNKY
                     GKESNNLRRGMGIKFLKLQEENRIRIEEFIRAFKAKVLAAGN"
     gene            complement(17048..17884)
                     /locus_tag="VEL68_03505"
     CDS             complement(17048..17884)
                     /locus_tag="VEL68_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HXZ35066.1"
                     /translation="MALSLESVGKTWGPYEFSYNERDLIIYALGIGFKKDNLEYVYEG
                     SKDFRPFPTYGVILPSNAGAEAFLSTKANFAMVVHGEQALQIHSPLPKGGTVQTTATL
                     EGVYDKGSGALIVMRFDTKEKGGTPVCTNWVSAFVRGSGGFGGQAQPKKNVPPIPQKN
                     PDFVFEAQTDMNQAALYRMSGDRNPLHIDPAIAKMVGFQEPILHGLCTYGVACRRFVQ
                     DVFEGDPGKLKSYSARFSSPVIPGETLQVKVWKTTPNLYLLEVFNAKGDAVIRNGVIE
                     AK"
     gene            complement(17990..19738)
                     /locus_tag="VEL68_03510"
     CDS             complement(17990..19738)
                     /locus_tag="VEL68_03510"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXZ35067.1"
                     /translation="MKTNLRLALGQINVTVGDLPGNARKIIEFMGQAREMGSDLVAFP
                     ELALPGYPPEDLLLNPAFVRDNLEALKKVTRATRGITAIVGFADMKDDIYNAAAILHG
                     GKMVAVYHKIYLPNYGVFDENRYFQSGEEFLVFELKRRAIGVNICEDIWYPGDPTRSQ
                     CLQGGATLIVNISSSPYHAGKRNSRQKMLSTRAWDYSTFVAYVNLVGGQDELVFDGGS
                     MVFDQRGELVAEAKAFEEELLVVDLPVDEVLLHRLHDPRRRKEKLVFPSERKLRWVRL
                     RAPKSEKPKNPLPPITPATLGRQEEIYRALVLGTGDYVRKNGFQKVVLGLSGGIDSSL
                     TAAIAVDALGEKSVVGVAMPSRYSSRESLEDAQSLAENLGIEFQVLPIDEVFAAYLKT
                     FEPSFTGKKPDVTEENLQARIRGNLLMGLSNKFGWLVLTTGNKSEVGVGYCTLYGDMA
                     GGFGVIKDVPKTLVYELAAYRNLLGPRPVIPCRVMEKPPSAELRPNQKDTDSLPPYNI
                     LDPVLQAYVEEDQSLETIVSRGFDRQMVAEVVRKVDLNEYKRRQASPGIKITPRAFGR
                     DRRLPITNRYRMKAKN"
     gene            complement(19810..21252)
                     /locus_tag="VEL68_03515"
     CDS             complement(19810..21252)
                     /locus_tag="VEL68_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ35068.1"
                     /translation="MERILIVDDDQSMRYSLNRMLEGQGLQLSLAKNGPEALERFEQD
                     RPDLVVMDIKMPGRSGLEVLREIKEKDPKALVILMTAFGTTETAIEAMKYGAFDYILK
                     PFDIPQMRGLVERALEVSRMMKKIVSLPDREKDEAAEETLVGSSSAMQQTYKMIGQVA
                     PTEVTVLLRGESGTGKEMVARAIYHHSRRGDRTFLPVNCAAIPETLLESELFGHEKGA
                     FTGALTRRIGKFEQCHGGTLFLDEIGDMTPATQAKILRVLQDRQFERLSGSERITVDV
                     RVIVATNKDLEKAIREGSFRQDLYYRLKVVTLHLPPLRDRKEDIPELVRYFLQRFRAD
                     INREVGDISPRALERLMRYHWPGNVRELENTVKRALVMAKGHTLLAEDFLLEGVDAGM
                     GPAEHLDLEERLQKLMEPVFKELLEVSRRNPGADLMSELEKILIKRALQETKGNQVQA
                     AILLGISRNTLRSKIERYRIRKDVAIVEEE"
     gene            complement(21396..24071)
                     /gene="amt"
                     /locus_tag="VEL68_03520"
     CDS             complement(21396..24071)
                     /gene="amt"
                     /locus_tag="VEL68_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXZ35069.1"
                     /translation="MNLSVLLQMLFLFFLLGSLWVAGGVLALEGETAGKPIKTLSDHA
                     ASMDMMWTLIASFLIFLMQAGFTLVEVGFTRAKNAGNVVMKNMIDFALGSIGFFLIGY
                     GLMFGASAAGLFGTGDFLLGHITSAGKVDNLKFANLMFQVVFAATAATIVSGAMSERT
                     KFIGYLFYSLLISMLIYPVVGHWVWGGGWLARLGMIDFSGSTVVHSVGGWVSLAGVLV
                     AGPRLGRYNRDGSVNLIPGHNIPLVALGVFILWFGWFGFNTGNTLSATNPSIALIAVN
                     TILAGAAGALCAMAVSWVLRGKPDVGMTLNGILSGLVSCTGGVAIISPFSAMAIGSVA
                     GFILYFSLSAFERRRIDDPVGAISVHGVNGIWGTLAVGLFAQDQYVQNSLGYAVNGLF
                     FGGGFNLLGVQTLAVVSVFLWAFPLSWFFFKMLDKTLGLRVSPEEEVRGLDFGQHSMT
                     SYPVFDEFQKKQEQILEELERVRELSLLHDIGQSMHTLNLEETLELILQGVAKGIGFD
                     RVRLYFLDEERKQLICKVAVGVEKEKIQSLTLPYDREDNMVSRAMMERRPFIVEDAGR
                     DPRVNLGLISFLDVKSFAVAPLLSRDRVLGGISADNLITQTVITENKLQSLMIFANQA
                     ALALENALMYEELKTFSSQLEGRVRKASARLEETQRQLFQSEKLAALGKLSAGIAHEI
                     RNPLTSIKILIHSLVDEQATETSREKDLAVIEAEIERVNKIIKQFLDFARPRPPSLEP
                     MEVKAILEETLALLVYEMEAQKISLERSYAPDIPLVPMDREQMKQVFLNLMLNAIQAM
                     ERGGHLRVAIRWRPAPSGDGGRSVAEITVQDTGPGMEEDIQRKIFEPFFSTKEEGIGL
                     GLPIARRIVEEHGGEVQVESRAGEGTTFTITLPFN"
     gene            complement(24294..25937)
                     /locus_tag="VEL68_03525"
     CDS             complement(24294..25937)
                     /locus_tag="VEL68_03525"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HXZ35070.1"
                     /translation="MSYSKPNASPATMSRNRVAAAPQSGICATCLDGCPGPCEVGRSA
                     LRGREVLYPQPYSKITAGAEKDYPVDLSHFNIQGTCVGAVGVSADSDRATFPSVDIST
                     TVGAKEKIKMKVPYFTGALGSTDIARIHWEPMAIAAAIAGTLVVVGENVCGMDPQAEF
                     KNGRVVKSPEMERRVRTFQKWYDGYGGIVVQHNVEDGRLGVPEYVVEKLGVEIIEPKW
                     GQGAKDIGGEVKLPTLERALQLKSRGYIVLPDPEDPVIQEAFQQGDFKEFERHSRLGM
                     VDEEDFHKEIKHLRKMGVKHISLKTGAYRPMDLARAVKYASDAKIDLLTVDGAGGGTG
                     MSPWRMMNEWGIPTVYLESLLYQYLKQLEKQKAYIPCCAMAGGLSLEDHIFKTFALAA
                     PYIRAICLGRASMTAAMVGGTIGEMIKKGKVPPEIGKYGMDVDHIFILAAQLKAKYGK
                     DFEKIPTGAIGMITYFDRLNAGLQQLMAGARKFALKYISREDLCSLTREGSDVSGISY
                     VMDVDRKEAVKILGQSAGGGNGRAQQKKKAAIVKEMALA"
     gene            complement(26200..26367)
                     /locus_tag="VEL68_03530"
     CDS             complement(26200..26367)
                     /locus_tag="VEL68_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35071.1"
                     /translation="MLRQIDFRAFWRKSPLQTGERYIPGVGTGAFSANGVDFFSFSES
                     LKIIGKRLDKN"
     gene            complement(26422..>26935)
                     /locus_tag="VEL68_03535"
     CDS             complement(26422..>26935)
                     /locus_tag="VEL68_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HXZ35072.1"
                     /translation="TQAMSEEYLKERSLKLGEGVVGYVFQENRPMMIYNVLKEPRYKE
                     KELAQKEGLVSMLSVPMCVKEKVIGVINCYTSYPHRFNKSEVDVFTTVANQAAVAIEN
                     AGLIMKAKMIEDELISRKLVERAKGILMKEHRLNEEEAFRRLQKKSMDLRKSMREIAE
                     AVILAHQIQS"
BASE COUNT         5672 a         7872 c         6839 g         6552 t
ORIGIN      
        1 gtccgtcaac tgccggtcat cgtacgacct cttttgatag acctgatagg cgcaagattt
       61 tttgacacac ttggcacagt ttaggcagcc atccccttcg atgatcccaa agggccccgg
      121 agggacgaat cgcgaggggg cgggtttcgg cttcagatgg tatttggagg gcatcttcac
      181 ctcaccagag gaaagatcag gaccttatac aaccgaccaa aattcagaaa cttctcattc
      241 cttatggcct tcagccgata ccttcaatct cttattttcc attttggaga gggccgatag
      301 ccccgaggga acgaatcttt tcaacccact tccccatttc ccgcacgttt tcatcggggc
      361 cctgatttcc cagcacgaac ctttgcaggc gatctttttc caacccgatt tcttccagga
      421 tcttcccgac gtgggccact cgccccttgg cccttgaact gcccaccaga taacggcagg
      481 ccccttccgg gcatccgaag agagccactc catcggcgcc gctttccaat gccttggtca
      541 gaagaataat ttccattttt ccggaacagg gaagaggaat ccggcgaaat ttaccacccc
      601 cggacccatc caatccagtg aggatttgat ggactttttc tccctgctgg gagcagtaaa
      661 aaacagttat atgaaccttt gaattttcca tgtcggcagt ttaggacgta aaaacttaag
      721 gccaaaacag cgggaggggc cgcagcccct cccgacaagc cgggttgctt accttctggg
      781 ggcagaggga atgacaaccc agcttgactt ctttgaacaa actcccctac aggataggga
      841 ggtacttatc aatctcgtag gaagtgatct ggatgcggta ccggtcccac tccaccttct
      901 tgttttcgat gaagtgttcg aaaacatggt cccccagcgc ttcccgcact atctggctct
      961 tttcggtaag ctggatggcc tccagcaggc ttcccggcag ctggcggatg cgccgcttca
     1021 gcctttcctc ctcgctcatc ttgtagacat tctcttccac tgggtcgggc gggatgacct
     1081 tctgctcgat tccggccagg ccggcggcca gcataaccga aaaggccagg taggggttgc
     1141 aggccgggtc cgggctgcgg aactcgatcc gggtcgcctc ttctttgccc ggtttgtaca
     1201 tggggacccg taccaggtcc gagcggttcc gctgggccca ggagagatac accggggcct
     1261 cgtaaccggg gaccaggcgt ttgtaggaat tgacccactg acaggtgatc gaggtgagtt
     1321 ccggcgcgta cttcaatagt cccccgatga aataccgggc ggtgtcggag aggtggtact
     1381 gtcccttggc gtcgaagaag gcgttccggt tgcccttgaa gagggacatg tgggtgtgca
     1441 ttcccgaccc attctccccg aataagggct tgggcatgaa ggtcgcatac actccctgcc
     1501 ggtaagccac ttccttcacc accaatcggt aggtcatgac gttgtccgcc atggtgaggg
     1561 cgtcttcata ccgcatgtcg atttcgtgct gggaaggggc cacttcatgg tgcgaatact
     1621 cgatcttgat ccccagggcc tcgcagaaaa ggaccgtctc ccgccgcagg tcgatggcct
     1681 cgtcccgggg gatcaggtcg aagtatcccc cccggtccag gacttccgtg gagtgggaat
     1741 ccttaaaata gaaatattcc agttcgggcc ctgtgtaata ggtgaagccc atcttcttgg
     1801 ccttctccag gttcttcttc agcacgaacc gcgggtcccc ttcaaacggt ttcccgtcgg
     1861 gggtgacgat gtcacagaac acccgggcca cgcccttctc ttttggacgc cagggcaaaa
     1921 tggcgaaggt ggaggggtcc gggatggcca ccatgtcgct ttcatcgatc cgcacgaacc
     1981 cctcgatgga cgacccgtcg aaaccgatcc cttcatcaaa agccttctcc atttcctcaa
     2041 tgggtacact gaaactcttg ataaacccca gaatatcggt gaaccaaatt ttcacaaact
     2101 tgacgtcgtt ctcttccatc ttcttcatga ttttggccgc tgtctctttc gccatctctt
     2161 tatcctcctg atttggtcag ctcgtgatca cgcgagtgat gatttgcagg tccttcattt
     2221 tctttctcag ggtgttgcgg ttgattccca gcctccgggc ggcacgcacc tgattgcccc
     2281 gttcttcttc caggagcatc ccgatcaaag cccgctccac tttgccgatg accgactcgt
     2341 ggatatggtc gtcgttgccc gttctgacct cggacttcac gacttcccgc acaacccctt
     2401 ccaacgtctg ggttggttct tccggttttt gctctttcgt cggaagcaga cgttgcaacg
     2461 gttcgggaag gttgttcccg gtcatccttg cttcgcccgg gggcaagatc ctctcgatca
     2521 ggccctggag ttcgtgatca tctttgatca caagaattgc tgccatagca atcaccccga
     2581 tttttttttg acctgttcca aagcaattca aataccaaca tgaaattctc cctatttttt
     2641 ttatcttaac aaatcaacaa cttaataacc taggccggcc gaacctaaag agcccttctt
     2701 gacctccttt cgactttctg cctaattatt gggcatcacc tgtatgatgc cacggttatt
     2761 agttaacaac atgtttattt tcagttactt gcagaaatgc atcgaaatta atcatcctgc
     2821 tcaaaatcgg tcatagcaaa gtgggaggtc ctactcccct ttgtcctccg aattatcagg
     2881 tttccggcct ttccagctct cgcaaaagtt cttctttaat cgcggatatt ttctgctcgt
     2941 cttcgatctt ggccccgctc atatcctgga cgtaaaatac atcgacgacc tgatccacct
     3001 tggtggaaat acgggccatc cacaggttta aacccatttc gaagagcttc tgggcaatgc
     3061 ggtagagaag gcccgggcgg tcgtgggtaa agacttccac aagagtgtaa aagtcggagg
     3121 tcacattatc cacttcgact tggggggcgg agcgcaccgt ggggtatcgg tggaagagtg
     3181 ggggaacgac ctcctgcagg agtcggtcca cctccacctt gccttccagg acttttttca
     3241 agtcctcctg cagcttcgtc caccggcgtg gctgaagcag cccttcctca atcaagctct
     3301 gaacccggaa ctgatccacg gcaatcccgt tctcccaggt actgatttgg gccgacagga
     3361 tattcaacaa attggccgtg aggaccccgc aaatcttggc aaacaggcct ggttcgtccc
     3421 gggccaccac ggtaatctca tctcccccct cctccggttt ttcttctccg tccagagcca
     3481 ccgcctggtc ggccagcttt tccgccatga ggacctgttt tccgatggct ctcgcttcgt
     3541 gcacctggta atggcgccgc ggaatgttga ccagatactc cgctatcttc gggggaggaa
     3601 tctggccttt gaccagctcc atgatttctc gctggactcc gatcacccgc tcctgatctt
     3661 ccctccggag cccttctcca cggtccagaa gatggagagt cttgaggaag agttcccgca
     3721 gcagagtgtc cttccacgcg gtccaggccg agggtcccac agcgcgcagg tcggcaaaag
     3781 caagcaggta gagcatcttc aaccgttccc ggtttcccac cgtttgggcg aagcggaaga
     3841 ttaggttttc gtcgttaagg tccctgcgtt gggcgatctc cgccaaggtc aagtgctccc
     3901 gcaccaaaaa gcgcagggta tcgatccttt ctgcggagag tccgaggcgt tcgccgacgg
     3961 attcagcgat cttttccccc cggagcgcat ggggagttcc ttcggccttg cccaggtcat
     4021 gaaccaaggc cgccaatttc aggagcgcgg ggtccgggac ctctctcatc agctgttcga
     4081 agagcggcca gggtggggga ccgttcttcc gttccagtat ttccagttcc tgcacggcat
     4141 acaaggaatg gacatccacg ggatagaggt gataccgatc gtactggatg tgacaatgaa
     4201 ctctttcgaa ctccgggagg aaccggcgca ggaacccggt ctcgtacatt tcttccaaaa
     4261 cccggtacag gtgtccgggc tgctcgaagt aagagagaaa ggaacggatg gattccgggg
     4321 cggagcgaaa ccgatcattc atcaggccca gcttctgggc gatgatctcc tgcagtccga
     4381 cgcttaattc tatcccgtgg cggtggacgt tctcaaaaac attccagatt tggagggggt
     4441 tccggtcgag gctcgccggg tcggtgaggg ccagccttcc gtggtacatg tggaagcccg
     4501 tggctatctc cggcgccgcc ttccgccacc aggtccgggt ggagtcctga acctcgttca
     4561 aacatttctc caggatgttc caggaaaggt gatgaacccg aagagcctgg tggaaataat
     4621 ctttgagaaa ggattccgta ttccggaggg agtcctctcc gcggtaaccg agaacttggg
     4681 ccaaggtggt ttggtcctcg aaggaaagcc ggtcctctct cctcccgtgc aggtaatgga
     4741 gctgattgcg aacccgccag agaaaatcca gagcctggga ataagtctcc cattctttct
     4801 cgctcaaaag gccctgctcg gccatctgcc gggcagaccg gagggagaat ttgattttcg
     4861 ccgcccagaa tgccgcgtgg taatcccgca gccctccttt cccgtctttg atttccggtt
     4921 ccaggacaaa aacagagtct cccaattttt tatggcggga ttcggcagat tgttgaatct
     4981 gtaggattac ttctttttcc tgacctcctt tgatcgcccg ggtaaactcc tcttcccagc
     5041 ggagaaaggc ccctttgtcc ccggccatcc agcgggcatc caggaaagag agaagaaggt
     5101 ccaggtcttt ttgggccgtt ttccagcttt ccttgggagt ttgcacggta tagcctacgg
     5161 tgagccccca atcccaaaga ggatgcagga ttttcttcac ccaggattcc aggttttttc
     5221 ctttgcctgg gaggtgaaga aaaaaaagat cgatatccga gaaggggttg agttcttctc
     5281 ttccgtaacc gcccatggcc aggagcgcga ctccctctcc ggtttctttt tcccccggct
     5341 cccggaaggc tcctttccag aggcgtcgga gtacattatc catcaacgcc gagcggctct
     5401 gacacatctc tgccccggca gcaccctcct catgccgctt cttcagccat tcctggccgc
     5461 tgcggagaaa cctctcgata tcttcgaggc tatctttggt cggttcggta gacatcttgc
     5521 gataaaatgt cctgcgggca aaaaggggaa aggcaaagag gcgaaacggg tcttgcggga
     5581 ggatgattcc gtcgctcttc tttccttttc gcccttcccc cttttcgcct ctctttacag
     5641 ggctccctcg ccccgctccc cggtccggat tcttaccgcc tcttccacgg gcaggacgaa
     5701 gatctttcca tccccgatct ttcccgtctt ggcagctttg gtgatcgtct ccaccacccg
     5761 cgtcacagcc ccgtccggaa cgatgacttc tattttgatc ttgggaacga gctccacgat
     5821 atactccgcc ccccggtaga cctccttgtg ccctttctgc cttccaaacc cccggacctc
     5881 ggtcaccgtc atcccctgga ccgccacgga gctgagggca ttcttcacct catccagctt
     5941 aaacggcttt atgatcgctt caactttttt cattcccccc cctcccgatt gctcattgcg
     6001 cattttaagg gtctcttcaa tatgcaatgt gcaatgcgaa attcacaatt ttagtagtta
     6061 tagcccgtct cgttgtgctg ggtaaggtct aaaccgctgt tttcctcctc ttcgttcaac
     6121 ctcaagccca tcgcccagcc gagtcccttc aacaggatga aggttaccgc aaaggcatag
     6181 gccatggtga ccaatacggc aacggcctga accccgaaga gctccgggtt tccccagagc
     6241 aggccgttat tgcctgcctc attgatttct ttcgtggcta aaagcccgat ggacagggcg
     6301 ccccaggtac cgcccactcc atggattccc accacatcca agctatcatc ataacggaat
     6361 ttattcttgg cgaatacccc cacgtaacag aaaacccccg ccgtgagccc gatgaacaaa
     6421 gccgccagcg gggtcacgaa acccgaagcg ggggttatgg tggccaaccc ggctaccgct
     6481 ccgctggcca ctcccagggt ggtgggtttc cccttgtacg cccattccac cagggcccag
     6541 gaaagagccc cggcggctgc ggcgaggtga gtcgccacga aggcgctggt gctcagcatc
     6601 ccggccgtaa tcgcgctacc ggcattgaac ccgaaccacc cgaaccagag gattccggtt
     6661 cccatcaggg tcatgggcaa actgtggggg gccatgggtt ccttcccata ccccttccgt
     6721 ttccccacca ggatggccgc cgccagagcc gaaatccccg agctgagatg cacgacgatc
     6781 ccccctgcaa agtccagccc tcccatcttg cgaatccatc ctcccacccc ccagacccag
     6841 tgggcgatgg ggtcatacac cagcgtactc cacaacagcg tgaaaatgaa gaaggcgctg
     6901 aacttcatcc gttctgcaat ggcccccatg agcaaagccg gggtgatgac cgcaaacatc
     6961 ccctggtaga tcatgaaggc ctgatggggc acggtctcgg cataatccgg attgggagcc
     7021 agaccgacac ccttcaggcc cagccaggag aggtccccga taatccctcc caccgtgggg
     7081 ccgaaggtga tactgtagcc gaagagcgcc cactggacgc tgatcagggc gatcaccata
     7141 aaactctgca tgatcgttcc aaggacattt ttggtccgaa ccatcccgcc gtaaaagagg
     7201 gccagtccgg gcgtcatcag catcaccaag gccgctgaaa tgaggatcca agccgtatct
     7261 ccggggttga ttttccccgc gggcgaaact gtcgaagcgg gttgaatcgc tgcgggtgtc
     7321 cccaccgtcg ccgggaccaa agttttgagg gccggggggt catccgccat tccgatgccg
     7381 ggccctagcc ataggagaat tcccaggacc aagacgcctt ttttcataaa cacctcccca
     7441 aggttggtgg attctcacct gcatcctggt caggggttaa tcaaccggcg tgccaacttt
     7501 tcaaggagcc agcagattca tcaagatgaa taaattcgga aagttaattt ttcaaggaga
     7561 gatatgtcta tgggtccccc tgctgatttt taaactcggt gtgcaaagac tgggcagaat
     7621 ggcgcccttt tccattgcga attttgcatt ggccattgcg cattcggcat tgcgaatggc
     7681 gctttcttcc cttcccttca atatgcaatt tacaatgcgc aatcagtatt tcacaaggct
     7741 ccctcgcccc tctccccggt ccggattctt accgcctctt ccacgggcag gacgaagatc
     7801 ttgccgtccc cgatctttcc ggtcttggca gctttggtga tcgtctccac cacccgggtc
     7861 acggccccgt cgggaacgat gacttctatt ttgatcttgg gaacgagctc cacgacatat
     7921 tccgcccccc ggtacacttc cttatgccct ttctgccggc caaatccttg gacctccgtg
     7981 accgtcatcc cctgcacggc aaccgaactc agggcggctt tcacttcatc caatttaaac
     8041 ggcttgacga tcgcttcgac ttttttcatg tcaacccccc cctcggaatt gcggattttc
     8101 gattgaggat tgcggattaa aaaagaaaac caaaaaaatc cccaatccga aatccgcaat
     8161 ccaaaattta aaaattgtac cccgtctcac tatgctggct caagtccagc cccatctctt
     8221 cttcttcctc gctgactctc aggcccaggg tccagtccag gacctttagg aggactaggg
     8281 tcacgacaaa ggcatacacc caggtcacca gcaccgccac cgcctgcacc ccgaataagc
     8341 ccgggtttcc gaagaagagc ccgttgtctc ccgcgtcatt gatggctttg gtggcaaaga
     8401 gcccggtggc cagggcgccc caggtgcccc ccacaccgtg tactcccacc acatccaggc
     8461 tgtcgtcata tccaaagcgg ttttttccca tcactccggc gtagcagaga attccggcgg
     8521 tcagcccgat gatcaaggcg gagatcggcc cgacgaaccc ggaagccggg gtaatggcta
     8581 ccagtcctgc caccgcaccg ctcgccgccc ccagggtggt gggcttcccc cggtagatcc
     8641 attcggctgc tggccaggaa agcgccgccg ccgcagtggc gatgtgggtg accacgaagg
     8701 cactggtgct cagctttcca gcggcgatcg cgcttccagc gttaaaacca aaccatccaa
     8761 accagagaat ggcggctccc atgatggtca tgggaaggtt atggggtccc atagggtccc
     8821 ggccgaatcc ctttctcttt cccaccagaa tcgccgcggc cagggccgat atgcccgagc
     8881 tgatgtggac gacggtcccc ccggcaaaat ccagcgctcc catcgaccga atccaccctc
     8941 ccacccccca gacccagtgg gcaatggggt cataaaccag agtcgcccag agaagggtaa
     9001 agacgatgaa ggttttaaac ttcatccttt cggcaatcgc tccggtaatc aaagccggag
     9061 tgatgatggc aaacatggcc tgatagatca tgaaagcctg atggggaatg gtagccgcgt
     9121 agtccgggta cgggtccagg cccacccccc gcagcccgaa ccaggaaagg cttccgatga
     9181 tgccccctac atcggggcca aaagccagac tgtaccccca aaggatccac tgaattgtaa
     9241 tgagagcgat gatgatgaag ctgtgcatga tcgtccccag cacgttcttc gtccgcacca
     9301 ttcccccata aaagagcccg agccccgggg tcatgagcag aacaagggct gcggaggcaa
     9361 gcatccaggc tgtatctccg gtatcaatct tcgggggcgc gggtgctgtc ggtgcagacg
     9421 ctgctggctt tgccgtttct gctgctccgg gagccggtcc ctccgctttg ggtttttcct
     9481 cggcgaaagt caggcccgca gatacaacca aagccatcat caggattatg gccatgaacg
     9541 atttcttcat gattctctcc tcccattttc catgttttta aatccctcta ccaggtatac
     9601 atcatcccga aggcaatggt atcctggctt ttcttgtcaa acgcattttt gtgcgaatca
     9661 aagcctttct ggtccgacca gtcgtgccgg tactccggcc ttaccaggag attcttggcg
     9721 atcttgaatt cgggagatag ggtgatctcc ttcagcttct gcgcgattcc cgtccgcacg
     9781 ccgttctggt catcgaagta ctccgcccgg atcgaagcgc taaagaagtc ggcaaactcg
     9841 tatttggcat acacggcagc gccataccat tctgaattat tgccgccgtt gttcgggtcg
     9901 ctgtcctcgc gggcataatc gatgttggcc atgaacgtca gttccttgct gggcttaatg
     9961 gctccgaccc agtcgaaaag aaaccggttg tgggaactgt tgttcgcctg ctcgggtccg
    10021 tacatgaaat tgaaggtaaa cgagaggatt tccgcgggcg taaagccaaa actggctccc
    10081 acggactttc cattattatt gtcggtcgtc acatcccagc cgttgactag gtagaggctg
    10141 gcactgaatt ccttagaaag ggcatactga gccatgaacc ccgtgtgggt aaaaggcacc
    10201 gcgtaattga aaaggaagga gcgggagtag ttaaaattgt ccctggcttc gatcacctcg
    10261 gcgctgtggt aggtggcgaa tttcccgaac cggagcttca ggccgcttcc cagaggggcc
    10321 acataatcga cataggcctc cgtgaggtca aactcatcat tggggtttcc caggcccgcc
    10381 gagtgtatgt atttggctgt ctccccggcc gagactttca gcttgaaccc caggccgccc
    10441 tccggtgcat ccttggcaaa ctggacctgg gccatgtcga tgaggaaggt gttggccttc
    10501 tggtcgaaga tcctctgccg gttgatcccc gagtcgggct tttcgaaatt gtacgtgtat
    10561 cccccctgga aatagaggct catgcccaaa agattcttaa tatcatccaa acttgtgacc
    10621 ggcttggcct ccggttttcc ctccgctttg gccgctggcg cagcctcagg cttcgcctct
    10681 tggccgcccg cgctcatggc cgatataatc actagaaaaa ctccaagaca tgtattcgcc
    10741 aatttatttt tcattttcat tcaccccaaa gacatgccct ttctgtcttt cctttggcat
    10801 gccaattttc aattggaatc ggccgtgggc gtttgccgat ccgcgaatcc atctgcttga
    10861 ctctttgagt ctgccattca agtcctgtgc cgcaagttca ttttttccct tcttccgtgg
    10921 aattcttgaa aattcatcgg cttagggaaa ccggcagcta aagaaagagg cggcagggat
    10981 gctgaatttt catccgcggc aggcgaccga aatcgttcgc caggcctgat aaatcagcaa
    11041 aaatcaaggc ttggatgatg ctgctgactc ttaaacagct tgggcaaaat tagggcagga
    11101 aggccgagaa ggaggaattt atcattgacg aaatttccaa aaattagtag gtttaggact
    11161 cgtgaagata ctcttgcctt ccttatcttt tggagagggc gggaggaagg aatctgacag
    11221 caggagagat tagcatgatg gattcaacgc cctatcagga gttctccaag atgttgggag
    11281 ctcctaattc caaggtcctt cctaggatcc tagaaaaaat cgctgacgaa aaagagttga
    11341 aggtccttat ggttgccttc cccccggcga cggtttcaga gttctcggag aaaaccggca
    11401 tcccccggga agaggtgcag gccatggtgc gaacgctctt ccagaaggga cttctttttt
    11461 cctccaaaaa agagggggaa ccccgctact accgggtcaa aacggtcccc cagtttcacg
    11521 acagttccat cctctggaag gaggcgacaa aagaattcca cgctctctgg aaggagtaca
    11581 cggacaagga atgggccgac ttcggaaagg tgatcgatgc ctttctccag aagccggcgg
    11641 tgcggatcat ccccgtggga gcgacgatcg aatcgaaagc caaagttctg gctttggagg
    11701 atgtgcggga aatcgtgaaa caggccagga ttttggcggt gaccccctgc acctgccggg
    11761 tggtggacgg aaaatgcggc aaacccgtgg aggtctgtat ccaggtcaac cgggcggcgg
    11821 attatgccgt ggaaagggga accgggcggg tcatcacccg ggaggaagcc cttcatatcc
    11881 tccaccaagc ggagaaggag gggctcattc atgtcgtcga caaccggcag aaggtggatc
    11941 atatcatctg caactgctgc cgggactgct gcatcctgca gcgccttccc aacccccata
    12001 aattcctcga ccccagccgc tttcaggcca tggtcaacgc ggaagagtgc atcggctgcc
    12061 aggcttgcct ggagcggtgc atgttcggcg ccattcggat ggaaggggac gagcccaaag
    12121 cccgggtctt cgctgaaagg tgcgtggggt gcggggtgtg cacgattggc tgccccacgg
    12181 aagccatctc catgaaggag gtccggcctc ccgatgttat cccggtttga ccttggagtc
    12241 cgtggcggga aaaattttgt tgagttcagg aatcaagaac ctactctatt cccgggttct
    12301 tccctttttc ctgatcatct tcttgaacga cgggtaagtt cacggttacc ttcgtccctc
    12361 cgcaatcgcc gcagtcaacg ttgatttccc cgtgatgatc gcgaatcaag gatttgcata
    12421 agaataggcc caaccccact ccgccctttc gggtcgaaaa aaaaggttca aatatccgat
    12481 ttcgctcgga actgggaatt cccggaccat catcgatcac ttcaatctgg gcccgattat
    12541 ccgaaggcag gaaccgggat tggagggtta tttttccccc agaggcaacc gcccccaggg
    12601 cgttcaaaaa caggttgata aaaactcgct ccaatttttc ggggtctgca tcgatcaacg
    12661 gaaggtcggc ggcgagtcgg gattccagga ccacccgcgc gcatttccta tccaagcggg
    12721 cggcgtccca cgcccgaaag aggagttcat ggatatccac cggttgccgg ttcaaaccgg
    12781 acttgatgaa atccaaaaat tgcctcagga gggaatttgc cctttgactg ctctcttgga
    12841 tcatcttcaa gcttttcttg agcgatcggg atagtttctc ctttgccaga caagattgag
    12901 aacaggattg gattacagcc aggggattcc taaggtcatg ggctaggccg gcgaccatgg
    12961 ctttgcccat catttcgatg cggggagtcc tttccacctg attccccaac ttctgaatcg
    13021 ccccgatcgc ctcgtttaga tattgcagta gaagatcgca cttcttcttg taatcctgga
    13081 gttccccctc aagctgccgc cgtcgaagga tttcctcctc caaggagtat ttttcctctg
    13141 aagagagagc ctggtttgac cgctttggtc ttgctagcct acccaccgtg ttcaaggaat
    13201 gatttcaccc ttgtcagccg acaagaaaac cccccgctcg caccctgctt ggatcatggg
    13261 gacgcgtgtc ctttggaccg cccacaattc gttgaacttt gcatacaggc ccgggggatt
    13321 cttctttcaa gccaggttgg atgcggaaaa gagggaaaaa cttcatcctt tttggatctt
    13381 ggtttccacc ccgttcaaag ctgatagata tttatccatg gcatttacta aggctcggaa
    13441 cggctcgagg gatgtccgta attcttcctt ggcttgcatt cgatccgtga tatcggtcaa
    13501 ggaatataat gctgctggtt ttttccccca ctgaatcagg gctcctcgac cttccaacca
    13561 acgagttcgc ccgtctttat gaatcacccg gaagggtttg ggctgagcgg gctctccggt
    13621 cttgagggtt tgggggagta tttctatcct ctcccgatcc tccgggagaa tcagatcgga
    13681 aaaggtcatg gaggccagct catctttggc atagcccaag acctccaacc ccttgggatt
    13741 gacaaatcga atgatccctt cttggacgac cgcaagggct tcacgggtgt tatccatcag
    13801 ggaattatat ttttcttcca ggctcccaat ccattcctca gtcctttttt gatgggtaat
    13861 atcaaatatc gaaacgatga tcctcttggt ttccggcatc ttcccgaact tgcaggagac
    13921 ctctcgaagg ttccctgctt tgtcccggaa ccggtactcc ccgtccgggg gaacctcttc
    13981 cagattctca tccaggcttc ccggacacct ttctttccct tctacatgac tcggggcaga
    14041 gaattcaggc cattttttct tgccttccac ttcctctttg gaataacccg agaatttctc
    14101 aaattccttg ttggccaagg agatgatctt gtttccttcc aggaggacaa cgggcgtcgc
    14161 cacattttca aagaccctcc ggaactcttc tgccccctgt agctcctcct ccgttcgccg
    14221 tcgttcgatc atttcctttt gcagtttttc attcaatttc tgaagctcgg gcttgagatg
    14281 gtccaccatt tcctctgaat gggtgaattt tagctttaac tcttcatcct tccgcctctg
    14341 ttcagagata tcccaagaga ccccaatgat gcccagaata tttccatttt cgttcttgaa
    14401 cggaatttta atcgttttcc aaacgaagga atgtccttcg cgaaccaaaa cttcttcgat
    14461 gctttctgag catcccgtag ccataattct ttggtcactg gttcggtatt tttcggccaa
    14521 ttccttcggg taaaaatcgt catcagtctt tcccgctatt tcctccggtt ttttctgcag
    14581 gtttgcagca aaggcccggt tacagagcag gtaaaccgaa tttttatcct tcatgaatat
    14641 tctttggggg aggttctcca agaggattcg gtaatggctc cccacctcct ggagttcttc
    14701 cagggccttt tgtcgctggg cctcggattc tttcaattcg agaatccctt ggcgcagggt
    14761 gaccatttca tttaccgctt tttcaatggt taagagcttt tccaaagtct cgtccgtttt
    14821 tgccatcgcc ctcatactcc tttcaatctt ttgagtggga aaacctcgga cccacaatcc
    14881 ctgtcattca gtttcctgca gcaagaactt tagccttaaa ggctctgatg aattcttcaa
    14941 tccgaatccg gttttcttcc tggagcttca ggaatttgat tcccatcccc cgtcgtaaat
    15001 tgttggactc cttgccatat ttattcgtcc agaccacttt gcactcccct tcggcgggct
    15061 cccggccatc gggtatgtgg attctcagaa gaaattcatc tcccaaatcc atcggattgg
    15121 gggtcaggac aaaagctccc cccagggtca agttgtcggt tacaccccaa tgctcttcat
    15181 caaactccac acggactttt gcttccactc tctcgtcctt ccggcgttca tcttttaaga
    15241 tgatcgcccc ctgggcccac gggactttgc cgttggtttt gttttcaagg aattcattgt
    15301 cgtccaattc ttgctccgct ttgatcatga taatatgcgt atcaatgcgt tgctgctcct
    15361 tttgggaaag gctttgcaac tgatttagga tattgtccag gtgtccctca taggcggtgc
    15421 gaaccgaact ccccttttga acttcctcga gcaaatcagc gatttctatg gcgtgggagg
    15481 caaaattggc caagctggag atgcatcgca gactcttctc atcgaagctg taacttccgt
    15541 tgatcagggc cacgctgatg actcccatgg gtttcccctt agccaaaaga ggaatgctca
    15601 ggagggaggt gattttattg tctcgttcct cgtatttgaa gaatcgggaa aaactctccg
    15661 ggccgctcag caataaaggg ctcccttgtc gaagggttct cttggcgatt tccttctcct
    15721 ccaccaatcc cacttgctga taccggggat caagagcatg ggtgaatttt attttcagag
    15781 cttcgccctt cgcctccagg agaaaaatgg tcgagcggtg tgccctcagg caattaatgg
    15841 actcctggga aatgacttcc aatattttgt ttgggtcttt atagcttttc aaggccgaac
    15901 tgacctggaa aatagccgtt tcaaaattgt caatgtactc tagggtctcc gaagcatcaa
    15961 gacctctgag agggccccct gttttggact gttttccgag gagttttccg aaaaccattt
    16021 tatgaatcgc ccctgtgcct aagaatgggt tgcatcagct caattctttt tttcccgatc
    16081 gctcgggaac gaacctcttc agccacaggc ttagaatgga attgccgggt ttccccccca
    16141 ggctggtttt tcaaggaacc cgcaaatttg ggggaaaaat atttctccct gtggataaga
    16201 gctccaaaga ggggaagatg gatttccctt tccttcccga tcaaggccgt acctttcttg
    16261 aaaatatcac tacccgctgg cgggtaatgg atggatcatt ccttagactt acaggttaat
    16321 ttctgatggc caaagcagtt tatgtcttcc tcaatcattg cccacgatac ttatcgtctc
    16381 acttccggtc atacttaaac cgatctttgt tttttttcag ctttagcccg gttttccctt
    16441 ttaggcctaa actcctccat cccggttatc ccccctccaa ggccttttct gggctttttg
    16501 agagtcttgt tctttgtcca agaagaaggc tgcatttaca ccaaattcat aaattaaatt
    16561 gataagttat tttgatttac cagattgctt gattgccctc aaaatttcag tggaatctct
    16621 cgccccgtgg ttaaaaaagg tcaatggaag gaaagattcc caagtggcag atttgctggt
    16681 tcttgcttct atgcaacact tacttcccca tattcttcgg tccccacagc gaaccagtgc
    16741 agttctggaa ggtgacgaac gagaagattt ccacctattt cgaaccctag ggtccccaaa
    16801 ttggaaggac gaaagggctc cttttttgaa cccttcttcc gcatcgatac taaagttggg
    16861 tagatcgaaa tcccctccca tattggaggg acccgctgaa tggtcggaat acataagaat
    16921 cgccggaaga aatattcaga cggcgggaaa ggaccttctt tggccttcaa gatagggcca
    16981 ggtcaggtct tatccgaact tcgattttca tagggcgact agccggtcgc ccctgctttg
    17041 aagggtccta ctttgcttcg atcactccat tgcggatgac ggcatccccc ttagcattga
    17101 agacttctaa caggtagagg ttgggggttg tcttccagac tttgacctgt agcgtttctc
    17161 cgggaataac cgggctgctg aatcgggccg agtaggactt caattttcct gggtccccct
    17221 cgaaaacatc ctggacgaag cggcgacagg ctaccccgta ggtgcagagg ccatggagaa
    17281 tgggttcctg aaagcccacc atcttggcta tggccgggtc gatgtgtaaa ggattgcggt
    17341 ctccggacat ccggtacagt gcggcctggt tcatgtccgt ctgggcctcg aagacaaaat
    17401 ccgggttctt ttgggggatc ggggggacat tttttttcgg ctgggcctgg cccccgaacc
    17461 cgccggaccc gcggacaaag gcacttaccc agttcgtaca gacgggagtt cctccttttt
    17521 ccttggtatc gaagcgcatg acgatcagcg ctcctgagcc cttgtcgtag accccttcca
    17581 gggttgcagt cgtttgcacg gtgccccctt tgggcagggg gctgtgaatt tgcagggcct
    17641 gttccccgtg gaccaccatg gcaaaattgg ccttggtgga caaaaacgcc tcggccccgg
    17701 cattcgaggg aaggattact ccataagtag gaaagggcct gaaatctttc gacccctcat
    17761 agacatattc caggttgtct ttcttaaacc cgatacccag ggcgtagatg atcaggtccc
    17821 gctcgttgta agaaaactca taaggccccc aggttttgcc gacggattca agactcagcg
    17881 ccatgttctt ctcctttcgc aagaggaatg gaatatcgtt gcgggttgct ccctaccggt
    17941 ctttcctttc tttctacaag cagcctgaaa ccagcaacca gtaactttct tagttcttcg
    18001 ccttcatccg ataccggttg gtgatcggta gccttcggtc cctcccgaaa gcccggggag
    18061 ttatctttat gcccggtgaa gcctgtcgcc ttttgtattc gttgaggtcc acctttcgga
    18121 cgacctcagc aaccatttgg cgatcaaatc cccgggagac aatcgtctcc aagctttggt
    18181 cctcttctac atatgcctgg agaacggggt ccaaaatatt gtaaggcgga agggaatccg
    18241 tatctttctg gttggggcgg agctcggccg agggaggttt ctccataacc cggcagggga
    18301 tgaccggcct gggtcccaat aaatttcggt aagcggccag ttcgtagacc aaggttttgg
    18361 gaacgtcttt gatcacccca aaacccccag ccatgtctcc atagagggtg cagtatccaa
    18421 cccccacttc actcttgttt ccggtggtca gaaccagcca cccaaatttg ttggagaggc
    18481 ccatcaaaag attcccgcgg atccgggcct ggagattctc ttcggtcaca tccggcttct
    18541 ttcctgtgaa ggaaggctca aaggttttca gataagcggc aaatacttcg tcaatgggca
    18601 agacctgaaa ttcgattccc aggttttcgg ccagagattg ggcatcctct aaactctccc
    18661 gggaggaata cctggaaggc atggccactc ccaccacgga tttttctccc agagcatcga
    18721 ccgcgatcgc ggccgtaagc gaagaatcaa tgcccccgct gagacccaaa acgacttttt
    18781 ggaatccgtt cttgcgtaca taatcccccg tgcccagaac caaagcacgg taaatttcct
    18841 cttgccgtcc cagggttgca ggcgttatag ggggaagagg atttttcggt ttctccgatt
    18901 tcggcgcccg caggcggacc caccgcagtt ttctttccga cggaaaaaca agtttttctt
    18961 tcctccgccg cgggtcatgg aggcggtgga gcaagacctc atctaccggc aagtcgacga
    19021 ccaggagttc ttcttcaaaa gctttggctt cagcaaccag ctctccgcgc tgatcgaaga
    19081 ccatggaccc cccgtcaaag accaactcat cctgccctcc taccagattg acataggcca
    19141 cgaaggtcga atagtcccag gcccgggtgg aaagcatctt ctggcgagag tttcttttcc
    19201 ccgcatggta cggagaagaa gagatattca caatgagggt cgctccaccc tggaggcatt
    19261 ggctccgggt ggggtccccg ggataccaga tatcttcaca gatgttgact ccaatcgccc
    19321 ttctcttcag ctcaaaaacc aggaattcct ctccgctttg aaaataccgg ttttcatcga
    19381 agactccgta gttggggaga tagatcttat ggtacacggc gaccattttc cccccatgaa
    19441 gaatggccgc ggcattataa atgtcatctt tcatatcggc aaaaccgacg atggcggtaa
    19501 tcccccgtgt ggcccgggtc actttcttca aggcctccag attatcccgg acaaaggctg
    19561 gattcaaaag gaggtcttcg ggaggataac cggggagggc caactccgga aaggccacca
    19621 ggtcggaccc catttcccgg gcctggccca tgaattcgat gattttccgg gcgtttcccg
    19681 gcaggtctcc caccgtgaca ttgatctgac ccaaggccag acgtaaattg gttttcattc
    19741 gtgcaatccc ttgttgaatt ttggcctgaa agccccaagg accgcatcac cccaggcgag
    19801 gggctcctat tattcctctt ccacaatggc cacgtctttc ctgatcctat atctttcaat
    19861 cttggagcgc aaggtgttcc ggctgatccc caaaaggatt gcggcctgga cctgattccc
    19921 cttggtttcc tgcaaggccc tcttgatcag gatcttctcc agctccgaca tgaggtctgc
    19981 ccctgggttt ctgcgggaca cctccagcag ttctttgaag accggttcca tcagtttctg
    20041 gagtcgttct tccaaatcca gatgctcggc cggccccatc cccgcatcca ccccttccaa
    20101 gagaaaatcc tctgccagca gggtatggcc tttggccatg accagggccc gcttgacggt
    20161 gttctccagc tccctcacgt tccccggcca atggtaacgc atcagccttt ccagagctcg
    20221 gggagaaatg tctcccactt cccggttgat atcggcgcgg aagcgctgca gaaagtagcg
    20281 caccaactcg ggaatgtcct ctttgcgatc ccgcaggggg ggcaggtgga gagtcactac
    20341 tttaagacga tagtaaaggt cctgccggaa ggacccttcc cggatggcct tttccaggtc
    20401 cttgttggtg gcgacgatta cccggacatc cacggtgatt cgttctgatc cgcttaggcg
    20461 ttcgaactgt cggtcctgca gtacccgaag gattttggcc tgggtggccg gggtcatgtc
    20521 tccaatctca tccagaaaga gggttccccc atggcattgc tcgaatttcc cgatacgccg
    20581 tgtcaaagcc ccagtaaagg cccccttttc gtggccgaaa agctcgctct ccagaagggt
    20641 ttcggggatg gccgcacaat tcactgggag gaaggtcctg tccccgcgcc gactgtgatg
    20701 atagatggcc cgggccacca tctcctttcc ggtgccgctc tccccccgca ggaggacggt
    20761 gacttccgtg ggggccacct ggccgatcat cttgtaggtc tgctgcatgg ccgaagagga
    20821 ccccaccagg gtttcctctg cggcctcgtc cttctcccgg tcgggcagag agacaatctt
    20881 cttcatcatc cgggagactt ccagagcccg ttccaccagc cctctcatct gaggaatatc
    20941 aaatggcttg aggatataat cgaaggcccc atacttcatc gcttcgatgg ccgtttcggt
    21001 tgtcccaaag gcggtcatca ggatcaccag ggccttgggg tctttttcct tgatctctct
    21061 caagacttcc agcccgcttc gcccgggcat cttgatatcc atgaccacca ggtccggccg
    21121 gtcctgttca aaccgctcca aagcctcggg gccgttcttg gccagggaga gctgtaagcc
    21181 ctgcccttcc agcattcgat tcagcgaata gcgcatgctc tgatcatcat caacgatcaa
    21241 aatgcgttcc atgggttctc gttacgggtt gctttttgct ggttgttcgt taaaaagaat
    21301 accttgttgt cggttgctgt ttgtgcgttg tccgttggaa aaaaggggga aatcaaaaat
    21361 aaccagtagc cagtaaccag caaccgtttt ccccttcaat tgaacggaag ggttatggta
    21421 aaggtggtcc cctcccccgc ccggctctct acctggacct ctccgccgtg ctcctccaca
    21481 atgcgccggg cgatgggcag gcccaggccg attccttctt ccttcgtaga aaagaagggc
    21541 tcaaaaattt ttctctgaat atcttcctcc attcccggtc cggtgtcttg gacggtgatt
    21601 tccgccaccg accgaccccc gtctcctgaa ggggccggcc tccaccggat ggccaccctc
    21661 aaatgtcctc cccgctccat ggcctggatg gcatttagca tcagattgag gaagacctgt
    21721 ttcatctgtt cccggtccat gggaacgagg gggatatccg gggcgtagct gcgctccagg
    21781 gagattttct gagcttccat ttcgtacacc agcaaggcca aggtctcctc caggatcgct
    21841 ttcacttcca tgggctccag ggacggcgga cggggtcggg caaaatccaa aaactgcttg
    21901 atgatcttgt tcaccctttc gatctccgct tcaatcaccg ccaggtcttt ctcccgcgag
    21961 gtttcggtgg cctgttcatc gaccagcgaa tggatcagga tcttgatgct ggtcagagga
    22021 tttcggattt catgggcgat tcctgcggag agctttccca gggcggccag tttctccgac
    22081 tggaaaagct gtcgctgggt ctcctccagg cgcgcggaag ccttgcgaac ccgaccttcc
    22141 agctggctgc tgaaggtctt cagttcctcg tacatcaggg cgttctccaa ggccagagcc
    22201 gcctgattgg caaagatcat gagggactgg agtttatttt cggtgatcac cgtctgggtg
    22261 atcagattgt cggcggagat ccctcccagc acccggtccc gtgaaagaag aggggccacg
    22321 gcgaaggatt tcacgtccag aaagctgatc aaccccagat tgacccgcgg gtcgcgacca
    22381 gcatcctcca cgatgaaggg ccggcgttcc atcatggccc gggagaccat gttgtcctcc
    22441 cggtcgtaag gcagggtcag gctctggatt ttctcctttt ccactcccac cgccaccttg
    22501 cagatcagct gctttctctc ttcgtccagg aagtagagcc tgacccggtc gaacccgatc
    22561 cccttggcca ccccctggag gatgagctcc agggtctcct ccaggttgag ggtatgcatg
    22621 gactggccga tgtcatggag gagggaaagc tccctcactc gttccagttc ttccaggatt
    22681 tgctcctgct tcttctggaa ctcgtcgaag accgggtagg aggtcatgga atgttggccg
    22741 aaatccagtc ccctcacctc ttcctcgggc gacacccgga gtcccagggt cttatccagc
    22801 attttgaaaa aaaaccagga aagggggaaa gcccagagaa aaacggaaac caccgcaagg
    22861 gtctggaccc ccagaaggtt gaaccctcct ccgaaaaaga ggccgttgac cgcatacccc
    22921 aggctgttct ggacgtactg atcctgagcg aagaggccca cggcgagggt cccccagatt
    22981 ccgttcaccc cgtggacgga aatggccccc accgggtcgt cgatgcggcg ccgctcgaag
    23041 gccgagaggg aaaaatagag aataaagcct gccacgctgc cgatggccat tgcgctgaaa
    23101 ggactgatga tcgccacgcc tccagtgcag gaaaccaggc cggagagaat cccattcagg
    23161 gtcatgccca catccggctt tccccgcagc acccagctaa cggccattgc gcagagggcc
    23221 ccagcggccc cggccaggat ggtgtttacc gcgatcagag caatgctcgg gttggtagcc
    23281 gaaagggtgt tgcccgtgtt gaatccgaac cagccgaacc agagaatgaa aacccccagg
    23341 gccaccaagg ggatgttgtg gccggggatc aggttgaccg atccgtcccg gttgtacctc
    23401 cccagcctgg gccccgcaac caggaccccg gccagagaaa cccagccacc caccgaatga
    23461 actacggtgg agccggaaaa atcgatcatc cccaggcgcg ccagccatcc tcccccccag
    23521 acccagtggc ccaccaccgg gtagatcagc atgctgatga gaaggctgta gaacagatac
    23581 ccaatgaatt ttgtcctctc gctcatcgcc ccggagacga tggtggccgc ggtggccgca
    23641 aaaaccacct ggaacatgag gttggcgaac tttaagttgt ccaccttgcc tgccgaggtg
    23701 atgtggccca gcaagaaatc ccccgtcccg aagagcccgg cggcgctggc tccaaacatg
    23761 aggccgtaac caatcaggaa gaaaccgatg gaacccaggg cgaaatcgat catgttcttc
    23821 atgaccacat tgcctgcgtt ctttgcccgg gtgaatccca cttccaccag ggtaaagccc
    23881 gcttgcatga gaaaaatcag gaaggaggcg atgagggtcc acatcatatc catggaggcg
    23941 gcatggtccg atagagtctt gatgggtttc ccagcggttt ccccctccag ggcgagaact
    24001 ccaccggcaa cccataggga ccccaataga aaaaagagga acagcatttg caggagaacc
    24061 gacaaattca tatttgaaaa ttttcttttg aacgcttgat cttcgctagc gctaggcccg
    24121 acgccctctg ctttatgcgg ccctggcttt ggtgatttac ccagttgtcg caatcttagc
    24181 actctttgag gacaaaaaaa aaggggaaag cctggacggc cttccccttt ggttagcgat
    24241 atcctaacgg gagttccgca ttatccctgc gctttcctcc cgcccatggt ccctcaagcc
    24301 agagccatct ctttgacgat cgccgctttc tttttttgct gagcgcggcc gttgccacct
    24361 ccggcggact gccccagtat tttgaccgct tccttcctgt ccacatccat cacgtaggaa
    24421 atccccgaaa cgtccgatcc ttcccgggtg agggaacaca ggtcttcccg ggatatatac
    24481 ttcaaggcga acttcctcgc cccagccatg agttgctgca ggcccgcgtt caggcggtcg
    24541 aagtaggtga tcatcccgat ggccccggtg gggatttttt cgaaatcctt tccgtatttc
    24601 gccttgagct gggcggccag gatgaagata tgatccacat ccatcccgta tttgccgatt
    24661 tccggcggga ctttgccctt cttgatcatt tccccgatgg tccccccgac catggccgcg
    24721 gtcatggagg cccggcccag gcagatggct ctgatgtagg gggctgccag agcaaaagtt
    24781 ttgaagatgt ggtcctccag cgaaaggcct ccggccatgg cgcagcaggg gatgtaggcc
    24841 ttttgtttct ccagctgctt gaggtattga tagaggaggc tttccaggta aaccgtagga
    24901 atcccccatt cgttcatcat cctccagggg ctcatccccg tcccccctcc tgctccgtcc
    24961 acagtgagaa ggtcgatttt cgcatccgag gcgtatttta ccgccctcgc caggtccatg
    25021 ggccggtagg ctccggtttt cagggagatg tgcttgaccc ccattttcct caggtgtttg
    25081 atctctttgt ggaagtcttc ttcatccacc attcccaagc gggaatgccg ttcgaactct
    25141 ttgaagtccc cctgctggaa ggcctcctgg atgaccgggt cctccgggtc gggcaatacg
    25201 atgtaccccc ggctcttcag ctgcagtgcc cgctccaggg tgggaagttt gacctcgccg
    25261 ccgatgtctt tggctccctg gccccatttc ggctcgatga tctcaacacc cagtttctcg
    25321 accacgtatt ctggaacccc cagtcttccg tcttccacgt tgtgctggac cacaattccc
    25381 ccgtatccat cgtaccattt ctggaaggtc cggacccgtc gttccatctc cggggatttc
    25441 accacccgcc cgttcttgaa ttccgcctga gggtccattc cacaaacgtt ttcgcccact
    25501 acgaccaaag tcccggcgat cgccgcagca atggccatgg gttcccagtg aatgcgggcg
    25561 atgtccgtcg atcccagggc cccggtgaaa taggggactt tcatcttgat cttttccttg
    25621 gctcctacag tggtggaaat atccaccgac gggaaggtgg ctcgatccga gtcggccgaa
    25681 acgccgaccg ctccgacaca agtaccctga atgttgaaat gggataaatc gacgggataa
    25741 tccttttcgg cgccggcggt gattttggaa tagggttggg ggtagagaac ctccctcccc
    25801 cgcagggccg accgccccac ttcgcatggc cctgggcagc catccaagca ggttgcgcaa
    25861 atgcccgact gaggggccgc tgcaactcga ttccggctca tggtagccgg gctggcattg
    25921 ggcttgctgt aagacatggg attcttctcc tttttgtggt ttttaagagt ccgtggcgat
    25981 gtccggtcgc gctaaaaaaa agcgccccct catccttccc gggacgcctt tgtcttccct
    26041 gccaacgacg ctcttcgtcg agcagattcc taggtttcgg ccgctaaaac gaaaaaaggc
    26101 atccgacctc gaggggggac gccttcgtct gttccgatcg cggcacatcc tcgtgccgcg
    26161 tcttttttaa ctaatctgcc gcttaattaa acataatgct caatttttgt caagtctttt
    26221 cccaataatt tttaatgatt cagaaaaaga aaagaaatcc acaccgttcg ctgaaaaagc
    26281 ccccgttccc actccaggaa tgtatctctc gccggtctgc aacgggcttt ttcgccagaa
    26341 cgcccgaaag tcgatttggc gcagcaccgg tatccctcca ccggagggca ccctcgaggg
    26401 ttcgaatgcc ttgtcaccgg gtcaggactg aatctggtga gccaggatga ccgcctcggc
    26461 gatctccctc atggatttac gtaggtccat ggatttcttt tgcagacgcc ggaacgcctc
    26521 ttcttcgttg aggcgatgtt ccttcatcaa gatccccttg gcccgttcca ccagttttcg
    26581 ggagatgagt tcgtcttcga tcatcttggc cttcatgatc agcccggcgt tttcgatggc
    26641 caccgcggct tggttggcca cggtggtaaa aacatccacc tcgctcttgt taaaccggtg
    26701 ggggtaggaa gtgtagcagt tgatcacccc gataaccttt tctttcacgc acatgggcac
    26761 gctgagcatg gaaaccagcc cttccttctg ggccagttcc ttttctttgt accggggttc
    26821 cttcaggacg ttgtagatca tcatgggcct gttttcctga aaaacgtatc ccaccacgcc
    26881 ctctcccagt ttgaggcttc tctctttcag atattcttca ctcatagcct gagta
//
LOCUS       DASPRR010000099         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1799847, whole genome shotgun sequence.
ACCESSION   DASPRR010000099 DASPRR010000000
VERSION     DASPRR010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799847"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VEL68_03540"
     CDS             <1..1035
                     /locus_tag="VEL68_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HXZ35073.1"
                     /translation="LPGQEQPVVRYLKEKLQPLAEEVHIGVNGNIYARKTGRKPGLKV
                     LVSAHTDEIGLVVRDIDSSGMIRFDKLGWFSDSFLPGTRVRVGKIPGIVGIKSGHLMT
                     EEEKRTVLPHRSLYVDVGAKTAADVEKMGISIGDPIAFDVPFGRWHDPDYCCAKAIDN
                     RAACAVAVALMESLTDDDFPGTLWVAGTVQEERGLGGARTAAQFVRPDWFLALDVALA
                     SDTPENPAGSEPVRLGGGVVISLGDFLESPKRGYFINPGLKELALRAGKEKSIPVQLQ
                     AIFGNSYTDAAAVSQEFSGIASISLGIPIRYSHAPSSVCHLGDMEACFRLAESMIRKG
                     VEKKDLDFLK"
     gene            1211..1975
                     /locus_tag="VEL68_03545"
     CDS             1211..1975
                     /locus_tag="VEL68_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35074.1"
                     /translation="MKLSEWVKNNSQTISVFVIMLLVWHFGVILLGIKEYILPTPWAA
                     IKTLFEAKYRWPMNFMATFYEVVGGFILSAAVGVFLGIAIVWSEWLKRTILPFLVFLN
                     TLPKIAVAPLFLVWFGYGILPNILIVILISFFPMVINTATGLVAVEEDLIDLVSYLHA
                     TKWQKLKLIQLPNSLPYMFSGLKITSTTAVVGAIVGEFIASDKGLGSVIISSQTTLST
                     PAIFGSLIYISVIGMGLFWAVEGLEKYLMPWERQNK"
     gene            2067..3068
                     /locus_tag="VEL68_03550"
     CDS             2067..3068
                     /locus_tag="VEL68_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35075.1"
                     /translation="MKKLIWALVIGTLVLGWGGWAPAADKVTLNLNWFYVGDHSPYFV
                     ATEKGWYKEEDLDVNIIPGKGSGDVVKKIDVGSAEFGIVDTGVLIVARAQGAKVKVVS
                     MLFDKSPYCMWMWKESGINSIKDLAGKKIGAPPGDAQRTIFPALAAANGLDPDKVTFV
                     NIGAEAKISALAAKQVDVIFDFYSGAPFYYKAIGQDKVTHLMFADWGVDVYSNAIVVT
                     DKYAQEKGNIIKRFVKASLRGWEFTLKNPEEAINIMAKYRTEIDKPVMLANLKLIIDL
                     FRTNRYKQNGIGWVEEKKMADSIKIISKYRDLKADMKTSDLYTNEYLTKIPLPIEVK"
     gene            3164..>3901
                     /locus_tag="VEL68_03555"
     CDS             3164..>3901
                     /locus_tag="VEL68_03555"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35076.1"
                     /translation="MNNRTPFINIRNLKKTYITKTGSVPALQDVTFDVYRGEFITLVG
                     PSGCGKTTLLKIISGLLTKSEGELDFSSKGDFDPRREVGMVFQKPVLLKWRSIVDNVL
                     LPIDILRLNRQDYHAKAMKLLDLVGLKGFEKSYPNELSGGMQQRASISRALIHDPQLL
                     LMDEPFGALDALTREKMNLEILRIWQETQKTVLFVTHGIQEAVFLADRVIVLSARPAR
                     MVAAIEVNLPRPRTMEIKAEKEFGKLCL"
BASE COUNT          931 a          966 c         1155 g          849 t
ORIGIN      
        1 cttcccgggc aggagcagcc tgtcgtccga tacctaaagg aaaagctgca acctcttgcg
       61 gaggaagtgc acatcggagt gaatggaaac atctatgccc ggaagacagg gcgcaaaccg
      121 ggcttgaagg tcttggtgtc agcccacacc gacgaaatcg ggctggtggt ccgggacatc
      181 gattcctccg gaatgatccg ctttgataag ctcgggtggt ttagcgactc cttccttccg
      241 gggacgcggg tgcgggtggg aaagattcct ggaatagtgg ggatcaagtc cggccacctc
      301 atgaccgagg aggagaagcg gactgttctc ccgcatcgaa gcctgtatgt cgatgtaggg
      361 gccaaaaccg ctgctgacgt ggagaagatg gggatttcca tcggggatcc catcgctttc
      421 gacgtgcctt tcggccggtg gcacgacccg gattattgct gcgcaaaggc gatcgataac
      481 cgcgctgcct gcgcggtggc cgtggcgctc atggagagtc tcacggacga cgattttccg
      541 ggaaccctct gggttgcggg aaccgttcag gaagaaaggg gattgggagg agcccggacg
      601 gcagcccagt ttgtccggcc tgactggttc ctggccctgg atgtagccct ggcgagtgat
      661 accccggaaa atccggcggg cagtgaacct gtgcggctgg ggggaggggt ggtcatcagc
      721 ctgggggatt tcctagagtc gcctaaaagg gggtacttta tcaatccggg tctgaaggaa
      781 ttggccctta gggcaggcaa agagaaatcc attcccgttc agctccaggc cattttcggc
      841 aattcctaca ccgacgccgc cgccgtttcc caggagtttt caggaattgc ctctatctcc
      901 ctgggaatcc cgatccgtta ttcccacgca ccttcttcgg tgtgtcacct gggagatatg
      961 gaggcttgct ttcggctggc cgaatctatg atccgcaaag gtgtggagaa gaaggacctg
     1021 gattttctga aatgaggtgt aagtgtaagt gaaggtgtaa aaacccgcag ataagcattg
     1081 ggaatgatgt aggggcaatt catgaattgc gccttcgaaa ttaggattag gcagcaggta
     1141 ggggcgggtt tgaaacccgc ccctatgttc cgtagaaggt tctggcaggt ttcatgagtt
     1201 gaggtggaat ttgaagcttt cggaatgggt gaagaataac tcccagacga tttccgtttt
     1261 cgtcatcatg ctgctggtct ggcatttcgg ggtgattctg ctggggatca aggagtatat
     1321 cctacccact ccatgggcgg ccattaagac cctcttcgag gccaagtacc ggtggcccat
     1381 gaacttcatg gccaccttct atgaagttgt gggcggattt attctatccg cggccgtggg
     1441 agtattcctg ggcatagcca tcgtctggtc ggaatggctc aagcgaacca tcctcccttt
     1501 tctcgtgttt ctgaatactc ttccgaaaat cgcagtagct cctctttttt tagtttggtt
     1561 cgggtatgga atcctgccca acattctcat tgtgattctc atctccttct tccccatggt
     1621 catcaacaca gccacggggc tggtggccgt ggaggaagac ttgatcgacc tggtaagtta
     1681 ccttcatgcc accaagtggc agaaactcaa gctgatccag ttgccgaatt ctttgcccta
     1741 tatgttctcc ggcctgaaga ttacctccac cacggctgtg gtgggggcta tcgtcgggga
     1801 gttcatcgcc tcggacaaag ggctggggtc ggtgatcatc tcttcccaaa cgaccctttc
     1861 cacgccggcc atcttcgggt ccctcatcta tatttcggtc atcggcatgg ggctcttctg
     1921 ggcggtcgaa gggctggaga agtatctgat gccgtgggag agacagaata aataaagaag
     1981 gggaaaagac gaaaagggga agaggggaaa gggtaaaaaa gcaaaataaa aggagcaaag
     2041 aaatggaaac aataaggaga gcgaagatga aaaagctgat ttgggcattg gtgattggga
     2101 cattggttct aggttggggc ggttgggcgc cggcggcgga caaggtgacc ctgaacctga
     2161 actggtttta cgtgggggac cattctcctt atttcgtggc caccgaaaaa ggatggtaca
     2221 aggaggagga cctggatgtc aatatcatcc ccggaaaagg atcgggagat gtggtcaaga
     2281 agatcgatgt ggggtccgcc gaattcggaa tcgtggatac cggggtcctg atcgtggcgc
     2341 gcgcgcaggg ggccaaggtc aaggtggtct ccatgctctt cgacaaaagc ccctactgca
     2401 tgtggatgtg gaaggaatct ggaatcaaca gcatcaagga cttggctggg aaaaagatcg
     2461 gggctcctcc gggggatgcc cagcggacga ttttcccggc cttggcggcg gccaacggct
     2521 tggaccctga caaggtaacc ttcgtcaaca tcggcgccga ggccaagata agcgccctgg
     2581 cggccaagca ggtggatgtg atctttgatt tctattcggg ggctcccttt tattacaaag
     2641 ccatcgggca ggacaaggtg acccatctga tgttcgctga ctggggggtc gacgtttatt
     2701 ccaacgccat cgtggtgacc gataaatacg cgcaggaaaa aggaaacatc attaaaagat
     2761 tcgtcaaagc gtcgctccgg ggttgggaat tcaccctgaa aaacccggaa gaagcgatca
     2821 acatcatggc caagtaccgc acggagatcg acaaaccggt catgctggcc aatctgaagt
     2881 tgatcatcga tctcttccgg acgaaccgat acaagcagaa cgggatcggc tgggtggagg
     2941 agaagaaaat ggcggactcc atcaagatca tttccaagta ccgggacttg aaagcggaca
     3001 tgaagaccag cgacctctac accaacgaat acctgaccaa aattccattg cccatcgagg
     3061 tgaaatgaaa aattcagcgg tcagcgatca gccatcagaa gtcagcaaat gcaggaaaat
     3121 ataaaaagct gaaggctgac tgccgaaagc taaaagcctt tttatgaaca accggacgcc
     3181 attcattaac attcgcaatc tgaaaaaaac ctacataacc aagaccggga gcgttccggc
     3241 ccttcaggac gtcacttttg atgtgtatcg gggagagttc ataaccttgg tcggcccgtc
     3301 cgggtgcggc aagacgactc ttctgaagat catctcagga cttctaacca agagcgaggg
     3361 agagctcgac ttttcttcga agggggactt cgaccccagg cgagaggtgg gaatggtctt
     3421 ccagaagccc gtcctcttga aatggcggtc catcgtcgat aatgttctcc tcccgatcga
     3481 catcctccgt ttgaaccgtc aggactacca tgcaaaggcg atgaagctcc tggacttggt
     3541 gggcttgaag gggttcgaaa agagttaccc caacgagctc tccggaggga tgcagcagag
     3601 ggcctccatc agcagggctc tgatccatga ccctcagctt ctcctcatgg acgagccctt
     3661 tggcgcgctt gacgccctga cccgggagaa gatgaacctg gaaatcctgc gtatctggca
     3721 ggaaacccaa aaaacggttc tctttgtaac tcacgggatc caagaggcgg tattccttgc
     3781 ggaccgggtc attgttctca gtgcccggcc ggcccgcatg gtggcggcca tcgaggtgaa
     3841 cctgccccga ccgcggacca tggagatcaa agcggagaaa gaattcggaa agctgtgcct
     3901 g
//
LOCUS       DASPRR010000100         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_676864, whole genome shotgun sequence.
ACCESSION   DASPRR010000100 DASPRR010000000
VERSION     DASPRR010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676864"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..1109)
                     /locus_tag="VEL68_03560"
     CDS             complement(414..1109)
                     /locus_tag="VEL68_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35077.1"
                     /translation="MLQVNEIHTYYGTSHILFGISLQVKEGEAVCLLGRNGAGKTTTF
                     RSIIGLSPPKSGSIQFREQQIVGKPPYRIAQMGIGFVPDTRRIFPDLTVRQNILVARR
                     EKEGSIWNLDRIYSLFPKLKVLDTHMGTQLSGGEQQMLTIARTLMTNPHLLLLDEPGE
                     GLAPLVIKAMEEQLGEIKKLGTTMLICEHNVGLATALSERAYVMDKGTIRYQGTIEEL
                     RKNEEVRKKYLMV"
     gene            complement(1109..1849)
                     /locus_tag="VEL68_03565"
     CDS             complement(1109..1849)
                     /locus_tag="VEL68_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35078.1"
                     /translation="MFLQTEELTKDFGKLRAVDQVSLEIRQGDIHAIIGPNGAGKSTY
                     FNLITGYHSASSGKVFYKGQEITKIPPYTRCRLGITRSFQITSIYPKLTVYESVLMAL
                     LSHRKVTLNFFTSAKKFFREEVWRILEEVGLADQADRLGDSISHGDKKRLELAVTVGT
                     EPELLLLDEPTCGMSPEETDITMGLVNKLSSERGITILFTEHDMGVVFGIARRISVLH
                     QGGLIADGTPEEVRASAEVQKVYLGGAK"
     gene            complement(1857..2831)
                     /locus_tag="VEL68_03570"
     CDS             complement(1857..2831)
                     /locus_tag="VEL68_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35079.1"
                     /translation="MEKRVGEKQESWLLKGWWLILLLLASLLAPVFLGEFYLTVLCEA
                     LVMSLLALSFNLLFGYMGQLSFGQAAFYGLGGYAVAMLMTKVQFNFWLSMVAGILVAA
                     LVGLLVGYFCVRLRGIYFAVLTLAFGQLIFFIVFKWHDFTGGDDGIQGVFPPEYLKSP
                     VAYYYFILVVFLAAAFVLWKIIQSPFGQTIKSMRENSERTEFLGINLAKYQLITFVIA
                     AALAGLAGAIWVPFYRSVAPSYLTWIKSGEPVMAAILGGPALFFGPVLGMFIMTFFHA
                     WVLGFTVYWPVVMGGLILFIIFFLPGGILGYTQEKLKERREKLNQARG"
     gene            complement(2843..3727)
                     /locus_tag="VEL68_03575"
     CDS             complement(2843..3727)
                     /locus_tag="VEL68_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35080.1"
                     /translation="MSFDVLFNQFMSGIARGSLLFLVASGLSLTFGVLRVLNFAHGSL
                     YMLGAFLSYSIWKYLLMPVLGFYLAAILAALVMAGFGLLIEFVLLRRLYARGWPEQLL
                     ATYAIVLVITDLVKWAWGGEFLSIPRPKPFTGAVFLFGFPFPSYNFFIIILGVGLVIF
                     LWWLVYKTRLGDIIRAAAHDREMVSALGIPIPWLFTWIFMLGAMIAGLSGAVTAPFGS
                     VALGMDIEIIVECFAVVVIGGMGSLPGTLLGSFIVGQVYAFGIMFKPELALAFIFMVM
                     AIVLIIRPWGIFGRPERQ"
     gene            complement(3888..>4720)
                     /locus_tag="VEL68_03580"
     CDS             complement(3888..>4720)
                     /locus_tag="VEL68_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35081.1"
                     /translation="TVDKFSPYFVQVVPNTYMEAIAATRYLQKKVPKAKKFATIGPDY
                     EFGRREEAAFTEEIKRLVPGVEIVYEAWPKLGEKDFTAFITAIMAKKPDAVHGSLFGG
                     DIVSFTKQAAPYGFFEKIPFIALYDFPVLLALGADAPEGTFGFGRGCFFMDPNPKMME
                     FAEKFQKATGAYPDAWAVQNYDSVYLLKAAVEKAKTVDTDAVVKAIEGMPFEGLRQGF
                     SIRALDHMGTVPCYQGTIAKDPKYPFKIWKDISRVPGEQVIRPEASVREIWQKTGVKR
                     "
BASE COUNT         1125 a         1285 c         1311 g          999 t
ORIGIN      
        1 ccggccccca caaagccgct ggtggccact gtatcgccgt cccggatgac ccgaaccgct
       61 tcctcggcag tgacgatctt gccttttttc agccgggggt gttggaaagt ggataatagg
      121 gggtgagaaa tcgctttttc aggggggctt tctttcatcg acaacctcca gttcaatgga
      181 tcgcgaaggg tgcgtgtccg cattcataag gtaaaggatg cggcctttgg ttggctctct
      241 cgcagggggg gcaggtgaga tctccgcagg ctccgaccga ggtacgggga gcccaaatgg
      301 gaggcggcaa gcgtagactt tgaccggagg gatatccttt tccccctgca accagagaag
      361 aataaaaacc gatcattcct gagatgaggg gctggccaag ctgcaggatt gttttacacc
      421 atcaggtatt tttttctcac ttcttcattc ttccgaagtt cctcgattgt tccctggtaa
      481 cgtatggtgc ctttgtccat tacataggcc cgttcactca aagcggtggc cagacccacg
      541 ttgtgttcgc agatgagcat cgtggtgccc aattttttga tctctcccag ctgttcttcc
      601 atggccttga tgaccagggg ggccaagccc tcgcccggct catccaggag gagaagatgg
      661 gggttggtca tcaacgttcg ggcgatggtg agcatctgct gttctccgcc gctgagttgg
      721 gtccccatgt gggtatccaa taccttcagc ttggggaaaa gcgaataaat gcggtccagg
      781 ttccagatcg acccctcttt ttctctccgg gcaacaagga tgttctggcg aaccgtcagg
      841 tcgggaaaaa tcctccgggt gtcgggaaca aacccaatcc ccatctgggc gatgcggtag
      901 ggtggcttgc ccacgatctg ctgctctcgg aactggatgc ttccagactt cggtggcgaa
      961 agcccaataa tactccggaa ggtcgtggtc tttccggccc cattccttcc caagagacag
     1021 acggcttccc cttcttttac ctgcaaagag atgccgaaga ggatgtgact agtcccgtag
     1081 taggtatgga tctcgtttac ctgaagcatt attttgctcc gcccaaatag actttctgca
     1141 cttccgcgct ggccctaact tcctccgggg tcccgtcggc tattaagcct ccctggtgga
     1201 ggacggagat ccgccgggca atgccaaata cgacccccat gtcgtgttcg gtgaagagaa
     1261 tggtgatacc cctctcggag gagagtttat tgacgagccc catggtaatg tccgtctctt
     1321 cgggggacat gccgcaggtg ggctcatcca ggagcaatag ctctggttcg gttcccacgg
     1381 tcacggcgag ttctaggcgc ttcttatccc cgtgagagat ggaatcgccc aaacggtccg
     1441 cctgatcggc cagccctact tcttccagga tccgccagac ctcttcccga aaaaatttct
     1501 tagcggaggt gaagaaatta agggtaacct tgcggtggga taagagggcc atcaatacgc
     1561 tctcgtagac tgtgagcttc gggtagatgc tggtgatctg aaaagagcgg gtgatcccga
     1621 gccggcacct cgtgtaaggg gggattttgg tgatctcctg ccccttatag aagacctttc
     1681 ccgaggaggc cgagtggtac ccggtgatga ggttgaaata ggtgcttttc cccgcaccgt
     1741 tcgggccgat gatggcatgg atatcgccct ggcgaatctc caggctgacc tggtccaccg
     1801 cccggagttt gccgaaatcc ttcgtcagtt cctctgtctg gagaaacata ggaaagtcat
     1861 cctctagcct ggttcagttt ttctcttctt tctttcaact tctcctgcgt atatcccagg
     1921 atgccgccgg gcagaaagaa aatgatgaac aggatcaacc ctcccatgac cacgggccag
     1981 tagacggtga agccgaggac ccaggcatgg aagaaggtca tgatgaacat acccaggacg
     2041 ggtccgaaaa agagcgccgg cccgccgaga atggcggcca tgaccggttc gcccgatttg
     2101 atccaggtta aataggaggg agcgacggat cggtagaagg gcacccagat tgctccggcc
     2161 agtccggcta gggccgcggc aatgacaaag gtgatgagct gatacttggc gaggttgata
     2221 cccagaaact cggtccgctc ggaattctcc cgcatggatt tgatcgtttg cccgaagggc
     2281 gattgaatga ttttccacag gacgaacgcc gcagccaaaa agacgaccag aataaaatag
     2341 tagtaagcaa ccggagattt cagatactcg ggggggaaca ctccctgaat tccatcgtcc
     2401 cctccggtga agtcgtgcca tttaaagacg atgaagaaga tgagctgccc gaaggcgaga
     2461 gtcaggacgg caaagtagat gccccgcaac cggacgcaaa aatatccgac aagaaggccg
     2521 acgagggcag ccaccaggat gcccgcgacc atgctcagcc agaagttgaa ctgaaccttg
     2581 gtcatgagca tggcgaccgc atatcccccc agtccgtaaa aagcggcttg cccaaaggag
     2641 agttgcccca tgtagccgaa aagaaggtta aagctcagcg ccaacaggct catgaccaaa
     2701 gcttcgcaca aaacggtcag gtagaattca cccagaaaaa cgggagccaa aagggaggcc
     2761 aggaggagaa ggatcagcca ccatcctttc agcaaccagc tttcctgctt ttctccaacc
     2821 cttttttcca tccaacattc tcctattgtc tttcgggcct tccgaagatt ccccagggcc
     2881 ggatgatcag gacgatcgcc atcaccataa agatgaatgc cagggccagt tctggcttga
     2941 acataatccc gaaggcatag acctgaccca cgatgaagga cccgagaagg gttcccggaa
     3001 ggcttcccat tccgccgatg accactactg caaagcactc cacaatgatt tcgatatcca
     3061 tacccaaagc cacggacccg aaaggtgcag ttaccgcccc gctcaggccg gcgatcatcg
     3121 cgcccagcat aaaaatccag gtaaagagcc atggaatcgg gattcccagg gccgaaacca
     3181 tttcccgatc atgggcggcc gcccggatga tgtcccccag acgcgtttta tagacgagcc
     3241 accagagaaa aatgactaat cccaccccca agatgatgat gaagaagttg taggagggaa
     3301 acggaaaccc gaagaggaat accgctccgg taaatggctt gggcctggga atggagagaa
     3361 actctccccc ccatgcccac ttgaccaggt cggtaatgac caaaacgatg gcataagtgg
     3421 ccagaagctg ctcgggccac cctcgggcat agaggcggcg gaggagaaca aattcgatga
     3481 ggagtccaaa tcctgccatc accaatgccg ccaggatcgc cgccaggtaa aagcccagaa
     3541 ccggcatgag gagatatttc catatgctgt aagagaggaa ggctccgagc atgtacagag
     3601 acccgtgggc gaagttgaga acccgcagga ctccaaaggt caaagaaagg cccgaggcaa
     3661 ccaggaaaag aagagagcct cgggcgattc cactcatgaa ctggttaaag aggacgtcga
     3721 aggacattcg cgcaacctcg cgtgatcggg cagttgaata ctcgggcgat ggttattgcg
     3781 ggccaagcga atcatgatcc ataatcgtag agacgttgcg cggggcaacg tctctacttt
     3841 gccgcttggt tctgggtaaa agggaaaccg gttttcccgg cagtcgctta tcttttgacc
     3901 ccggttttct gccagatctc tctcacactg gcctcaggac ggatgacctg ctcgcctgga
     3961 acccgagaaa tgtctttcca gatcttgaag gggtacttgg ggtccttggc aatggttccc
     4021 tggtagcagg ggaccgttcc catatgatcc aaagcccgga tggaaaagcc ttgccttaat
     4081 ccttcgaaag gcattccctc gatggccttc accaccgcat cggtgtccac agtcttggcc
     4141 ttttcaacgg ccgctttgag gagatagacg gaatcataat tctgaactgc ccacgcatcc
     4201 ggataagccc cggtggcttt ctggaacttc tcggcaaatt ccatcatctt ggggttgggg
     4261 tccatgaaga aacatccccg gccgaatcca aaggttcctt ccggggcatc ggcccccagg
     4321 gcgagcaaca cggggaaatc gtaaagggcg atgaagggga tcttttcgaa gaaaccgtaa
     4381 ggggcggcct gcttggtgaa ggagacgata tcgcccccga aaagagaacc gtgaacggca
     4441 tccggtttct tggccatgat ggcggtgatg aaggcggtaa aatctttttc tcccagtttg
     4501 ggccaggctt cgtagacgat ttcaaccccc ggcaccagcc tcttgatctc ttccgtaaag
     4561 gcagcctctt cccggcggcc gaactcataa tccggtccga tggtggcgaa ttttttggcc
     4621 ttggggacct ttttctggag atagcgcgta gccgcaatgg cctccatata agtgttggga
     4681 accacctgaa caaaataggg ggagaacttg tccacggtct
//
LOCUS       DASPRR010000101         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2121264, whole genome shotgun sequence.
ACCESSION   DASPRR010000101 DASPRR010000000
VERSION     DASPRR010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121264"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VEL68_03585"
     CDS             complement(<1..785)
                     /locus_tag="VEL68_03585"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXZ35082.1"
                     /translation="MPEAVIVEAVRTPIGRHGGILKEVRPDDLAAIVLAEVVKRAKID
                     PAIVEEVYLGCANQAGEDNRDVARMALLLAGFPFTVGGVTFNRLCASGLTAINSASRA
                     IKAGEGEVFVAGGVECMTRAPYVMAKTYPEWNFGNQTLWDTTIGWRFPNPRMKERYGI
                     DSMGETAENVYELTRSISREEQDRFSLESHRRAVAAIESGRFAEEIVPVSVPQKRGEP
                     LLLKRDEHPRADTSLEALGKLRPAFRAGGTVTAGNSSGINDGAA"
     gene            1313..2380
                     /locus_tag="VEL68_03590"
     CDS             1313..2380
                     /locus_tag="VEL68_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXZ35083.1"
                     /translation="MEYRKMGRTGLKVSAFCLGTMTFGRQVEEKESLRIIDRALDAGV
                     NFIDTADMYVNGVTEQIVAKAIKGKRDALVLASKAGHLRKLAAKYGEQKVSGPIDLAR
                     PRPFTPWPGGEGTGPNDMGLSRKHILQAVEGSLRRLGTDYLDIYYAHMPDYDTPLEET
                     IRAMDDLVRQGKVRYLGCSNFRAFQLCKALWISDKHNLARWDVIQPPFNLITRDIEYE
                     LLPLCREEGIGVCPFSPMAAGLLTGKYEKGKAPLEGARFSLAKWGYTYNQKYWDELNF
                     AAVEQLGKIAAGSGKSLPQFALAWVLNTPGITSIVNGATSMPQLEENLGAVDIKLSKE
                     EMEACDEVWKMLRPPRLFYGR"
     gene            complement(2530..3807)
                     /locus_tag="VEL68_03595"
     CDS             complement(2530..3807)
                     /locus_tag="VEL68_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ35084.1"
                     /translation="MIIFLAAFIALLCISAPITVALGGCSLVYALVGGTVPITTLIQT
                     TFGGLTSFPLLAIPLFMLAGNLMNEGGLTPDLVNFARNLLGHIRGGLGHATILACAIF
                     AAISGAAVATAVAIGVVMLPAMKKAGYDEGVAAALTCTAACLGPIIPPSIPFIIYGVS
                     ANVSIAALFVAGVLPGLVLAAALMVYMHFVATRRNYPRDPKKSGKEILIAGGKALPAL
                     FMPVLIMGGILSGMFTPTEAAGVTVVYSTVMGAFYYRQLNFKNLPRVFLKSGLESGMV
                     MLLIAMSEPFAWFVAADQIPQLIIEWISQVSTSPYVILFLINVFLILLGIPMETAPAL
                     VIVTPILVPIGAAVGIDPVHLGIVVCLNLVLGLITPPVGAVLFAVCGMTGMSLDRLSK
                     SIWPPFFVAVGVLAIVTYLPWLSTYLPRLVLGR"
     gene            complement(3804..4301)
                     /locus_tag="VEL68_03600"
     CDS             complement(3804..4301)
                     /locus_tag="VEL68_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ35085.1"
                     /translation="MSALRGFNEILIKIGSWGTILFLAVIAVVIPYEVVGRYVFQKMS
                     IWSGEVSTYSLAWASMLGSAVGLKKGYQVSMTSVVESVPPPVAKILRWGAYLFTLFFF
                     AALFYFGLYQTTYNARQTSPAIGLAMSIPYASLPAGFFIMFFLTLEEFLAFITPDRRG
                     KGEGQ"
BASE COUNT         1099 a         1311 c         1398 g          869 t
ORIGIN      
        1 gctgccccgt cgttgatccc tgaggaattt cccgcggtca ccgttccgcc ggcgcgaaag
       61 gcgggccgca gtttgcccaa tgcctccagg gatgtatcgg cccggggatg ctcgtcccgt
      121 ttgagcaata ggggttctcc ccttttctgg ggaacagaga cgggcacgat ctcttcggcg
      181 aacctgcccg actcgattgc cgccacggcg cgacggtggc tttcaaggga aaaccggtcc
      241 tgttcctccc gggagatgga tcgggtgagc tcgtagacgt tttcagcggt ttcccccatg
      301 gaatcgatcc cgtatctctc tttcatccgg ggattgggaa aacgccagcc gatggtggtg
      361 tcccacaggg tctgatttcc gaaattccat tccggatagg ttttggccat gacatagggg
      421 gcccgggtca tgcattccac ccctccggca acgaagactt ccccttcccc ggccttgatg
      481 gcccgggatg cggagttgat ggccgtcagc ccggaagcgc atagccgatt gaaggttacc
      541 ccacccaccg taaaaggaaa accggcgagg agaagggcca tccgggccac atcccggttg
      601 tcctcccccg cttggttggc gcaccccagg taaacttcct ccacgatggc gggatcgatt
      661 ttggcccgct tcacgacctc ggccaaaaca atagccgcca ggtcgtccgg gcgcacttcc
      721 ttcaggattc ccccatgacg tccgatgggg gtccgcaccg cttccacaat gacagcttcg
      781 ggcatgctta aatcctcctc gcagaatcaa tcagagattt ggcttgcctt aaaatccctt
      841 atctttataa tctaatctga aacgctgtgt caactcatgg tctcccttgc ccacttttgc
      901 gactggcttt tctgcgaaaa agcaagctgg tggcccggaa gcaagggcgt tgcgcgcaaa
      961 aagcatcggc gggaagagaa aaccattcgg agattccccc gtgcttccgc agaatgaggc
     1021 gggcccatcg ggcggtcggg cgtccctaca tcggactaat cctcttcctg gggaaagaga
     1081 ggcttgcccg cccgttccaa gaagggaggc gagcctcatg gtttttgcgc gcaacgccct
     1141 tgcttccggg cccggcagag gccgaaattc aacaggattg acaaacactt gccgggaagg
     1201 ggagggggca agacgactcc ggtttaaaaa ggcggggtga aataaattcc gtttagggtt
     1261 atcaaggaaa gagatatcgg tttcatcttg tgatggatga gggggtcatc gaatggaata
     1321 cagaaagatg ggaaggacgg ggctgaaggt ttcggctttc tgcctgggca ccatgacttt
     1381 cggtcgccag gtggaggaga aagagtcact ccggatcatc gatcgagccc tggatgccgg
     1441 ggtgaacttc atcgataccg ccgacatgta cgtgaacgga gtgaccgagc agattgtggc
     1501 caaggccatt aagggaaagc gcgatgccct tgtgcttgcc tcgaaagccg gccacctgcg
     1561 caaactggcc gccaagtacg gagagcagaa ggtatccgga ccgattgacc tggcacgacc
     1621 gaggcccttc accccttggc ccgggggaga ggggacgggc ccaaatgaca tgggtctttc
     1681 ccgcaagcat atcctgcagg ccgtggaagg aagtctaaga aggttgggaa cggattacct
     1741 ggacatctac tacgcccaca tgccggacta tgacacgccc ctggaggaga caattcgggc
     1801 catggatgac ctcgtccgtc agggaaaagt ccggtacctg ggctgctcca atttccgggc
     1861 ctttcaactc tgcaaagccc tgtggatcag cgacaagcac aacctggccc gatgggatgt
     1921 tatccagcct ccctttaacc taataacccg cgacatcgaa tacgagttgc ttcctctctg
     1981 tcgggaggag gggatcggcg tgtgcccctt cagccccatg gcggccggtc tcctgacggg
     2041 aaaatacgaa aaaggcaaag cccccctgga aggagcccgc ttcagcctgg ccaaatgggg
     2101 atacacctat aaccagaaat actgggacga gctgaatttt gccgccgtgg agcaattggg
     2161 gaaaatcgcc gccggaagcg gcaaaagcct gccccagttc gccctggcct gggtgttgaa
     2221 cactccggga atcacctcca tcgtcaacgg ggcaacctcc atgccccagc tggaagagaa
     2281 cctgggggcc gtcgatatca agctctccaa agaagagatg gaggcctgcg atgaagtgtg
     2341 gaagatgctg cgcccgccga ggttgtttta cggaagataa aaagagaaaa tgggacgcag
     2401 atgaacgcag attttactcc gtgaacggcc cgatcaggga tcgggcccta caatttgaag
     2461 aacaaatgta gggcgcgttc cccgaaggcg ccgattcgat tgggaacata aaattgatat
     2521 gccaggggcc taacgcccga gcacaagtct gggaagatag gtgcttagcc agggcagata
     2581 ggtcacgatg gccagcacgc ccacagccac aaaaaatggc ggccagatgg acttgctcag
     2641 gcggtcgagg gacatgccgg tcatgccgca gaccgcgaag agcacggcgc cgaccggcgg
     2701 cgtgatcaga cccagaacga gattcaagca gacgacgatg cccaggtgga ccggatcgat
     2761 gccgaccgcg gcgccgatgg gtaccagaat cggggtcacg atgaccagcg ccggggcggt
     2821 ttccatgggg atgcccagaa ggatcaaaaa gacgttgatt agaaacaaga tcacgtaggg
     2881 cgaagtgctc acctgtgaaa tccactcgat gatgagctgg gggatctggt ccgcggcgac
     2941 aaaccaagcg aaaggctccg acatggcgat gagcagcatc accatgcccg actccagtcc
     3001 ggatttcagg aataccctgg ggaggttctt gaagttcaat tgccggtagt aaaaagcccc
     3061 catgacagtc gaatagacca cggtaacgcc ggctgcttcc gttggcgtga acatgccgct
     3121 caggataccg cccatgatca ggaccggcat gaacagggcc ggcagagcct tgccgccggc
     3181 aatgaggatt tcctttccgg atttcttcgg gtcccggggg taattacgcc tggtggccac
     3241 gaagtgcatg tagaccatca aagctgctgc tagcacgagg ccggggagca ctccggccac
     3301 gaaaagcgcg gcgatggaga cattggcgga gacgccgtag atgatgaacg ggatgctggg
     3361 cgggatgatc gggcccaggc aggcagccgt acaggtcagg gcagccgcca ctccctcgtc
     3421 gtagcccgcc ttcttcatgg ccggaagcat gacgaccccg atggccactg cggttgccac
     3481 ggctgccccg gagatggccg cgaagatggc gcaggccagg atcgtggcat ggcccaggcc
     3541 gccgcggatg tgcccgagga gattacgggc gaagttcacc aggtcgggcg taaggccgcc
     3601 ttcgttcatg aggttgccgg ccagcatgaa aagggggatc gcaaggaggg gaaaactcgt
     3661 caaaccgccg aaagtggtct ggatcagcgt ggtaatcggg acagtgcccc ctaccagggc
     3721 gtataccagg gagcaaccgc cgagtgctac agtgatgggg gcggagatac acaacaaggc
     3781 aataaaggcg gccaggaaga tgatcattga ccctctccct tccctctcct atccggggtg
     3841 atgaacgcca agaattcctc cagcgtcaaa aagaacatga tgaagaaccc cgctggcagc
     3901 gaggcatagg ggatactcat cgccaggccg atcgcgggcg aggtctgcct ggcgttatag
     3961 gtggtctgat agaggccgaa gtagaatagg gccgcgaaaa agaacaacgt gaagagatag
     4021 gctccccatc tcagaatctt ggcgactggg ggcggaaccg attccaccac cgacgtcatg
     4081 ctgacctgat agcctttttt caaccctacg gcgctgccca gcatggaggc ccaggcgagg
     4141 gaataggtcg agacctcgcc cgaccagatg ctcatcttct ggaaaacgta acgtccaacg
     4201 acctcataag ggatgacgac ggctatgacg gccaggaaga ggatcgtccc ccagctccct
     4261 atctttataa gaatttcgtt gaaccctctc agcgcgctca tcaagcctcc tccactgaat
     4321 tctcaaaaac ggcataacca atacaagcga taaataaagt accatcaccc cctccctaac
     4381 cctcccccct tggagactgt gtcgtaattc gtcatgccag cgaaagctgg catccaggat
     4441 atcgaaaaat tgaataaaat caaaaactgg attccggtcg gagactcgac tctacgagct
     4501 ttcgccggaa tggcgactct tttccccaat tgcgacacag tctcttgagg gggagggaag
     4561 ggtggggggt attatgaatt atttcgtctg tattaggcgg gagggaaaaa ttttcccccg
     4621 cggccttata gcggcacaag gagattcccg gcagtcagag gatttccccg tggcgcc
//
LOCUS       DASPRR010000102         5385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_28823, whole genome shotgun sequence.
ACCESSION   DASPRR010000102 DASPRR010000000
VERSION     DASPRR010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28823"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VEL68_03605"
     CDS             complement(<1..414)
                     /locus_tag="VEL68_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HXZ35086.1"
                     /translation="MKITSIDSAVYNVPLSVPVSDSTHGEMASFELITTRIKDDQGCE
                     GLGYTYTVGVGGLAIHALIDRYLKPGLLGQDPGRIEQLWNWMWWKLHYAGRGGPASFA
                     ISAIDIALWDMAGKRRGEPLWHLLGGNNPRVKVYAG"
     gene            complement(664..2199)
                     /locus_tag="VEL68_03610"
     CDS             complement(664..2199)
                     /locus_tag="VEL68_03610"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXZ35087.1"
                     /translation="MAIRGDRIARIGRVEKGEATREFSAAGAVVSPGFIDTHSHSDLM
                     ALAEPALLPKLMQGVTTELLGQDGIAAAPIRKGYVAPWRQYLSGLTGDPSIPWDWESI
                     GDYAERLAAKPTGSNLAFLVPQGNIRMVVLGLENVLPDVFQVQAMEEEVRKAMEEGAA
                     GISLGMVYMPCTFSRRDELLRLLHTCGCMGGFLAVHIRSGSDRLLEAIEEVVGLAREA
                     EIPLHISHFKASGKRNWHKMETALEAVDRAHREGLDITFDIYPYTAGSTMFLAILPPW
                     VLEGGPAKALERLRDPALRAKIRDQFINPPSPDPEGPGWDNFVNNVGWENIIISSAEG
                     KKNRDWLGRSVADIAEREGKDPAEIAFDILLAEDARVGMILFSMDEEKMVLGLRHPLG
                     MICTDGILGGRPHPRVYGTFPRILGKYVREKKSISLEEAIRKMTSLPARRLGLKDRGV
                     LTEGKAADIVVFNPATVTDRATYDHPKQYPIGIRQVIVNGVLSVEGGEHTGKLGGKVL
                     KRT"
     gene            complement(2402..4045)
                     /gene="pgm"
                     /locus_tag="VEL68_03615"
     CDS             complement(2402..4045)
                     /gene="pgm"
                     /locus_tag="VEL68_03615"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HXZ35088.1"
                     /translation="MSTHPLAGKPAPPEALIDVARLERAYYDKRPEIEDPNQRVSFGT
                     SGHRGNPLEGSFNEAHILAITQAICEFRRRKGISGPLFMGKDTHAISNPAQKTALEVL
                     AANDVATFIQSHEGYTPTPVISHAILINNRGRKEGIGDGIVITPSHNPPADGGFKYNP
                     PNGGPADTDVTGWIQDRANALLRSTNREVKRVSYNQAIKASTTQEMDFIEPYVKDLSN
                     VLDMEVIRSAGVQIGVDPLGGSSIGYWEPIKEHYQLNLTVVNPRIDPTFGFMTLDHDG
                     KIRMDCSSPYAMAGLVELKDQFQVAFGNDTDSDRHGIVTRTAGLMNPNHYLAAAISYL
                     LSRRSKWPAASMVGKTLVSSSLIDRVVEKFGRKLFEVPVGFKWYADGLLKGSLCFGME
                     ESAGASFLRKDGTVWNTDKDGILLGLLAAEMTARAGKDPGEYYQELTARLGVPYYLRI
                     DAPATPEQKSRLKGLAPEAISVRELAGEPILAKLTRAPGNGGSIGGLKVVTPNGWFAA
                     RPSGTENIYKLYAESFKSQTHLEGIVEEARGILSRVLEE"
     gene            complement(4061..4726)
                     /gene="rpe"
                     /locus_tag="VEL68_03620"
     CDS             complement(4061..4726)
                     /gene="rpe"
                     /locus_tag="VEL68_03620"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXZ35089.1"
                     /translation="MIKIAPSILSADFLKLGEEIQAVERAGADRLHIDVMDGRFVPNI
                     TMGIPVVRSLRRGTRLPFEVHLMIEQPENYLEAFAMAGADLLIVQQENSVHLDRTLQA
                     IKELKKKAGVALNPSTPAGMLEEIIEKLDLILVMTVNPGFGGQKFMEYPLKKVRLLRE
                     ILDRRNRNCEIEVDGGIAPQNAARVVEAGAGVLVAGTSVFGHPEGPEEGVRSLMQAVM
                     GKG"
BASE COUNT         1075 a         1634 c         1447 g         1229 t
ORIGIN      
        1 cccggcatag accttcaccc gcggattgtt cccgcccagt aaatgccaga ggggttctcc
       61 ccttcttttt ccggccatat cccacagagc aatgtcgatc gccgagatgg cgaaggaagc
      121 cggccccccg cgtccggcat aatgcagctt ccaccacatc cagttccaca gctgctcgat
      181 cctgccaggg tcctggccga ggagacccgg tttcaggtac cggtcgatca gggcatggat
      241 cgccagcccg cccaccccca cggtgtaggt gtaaccgagc ccttcgcaac cctggtcatc
      301 cttgatccgg gtggtgatta attcaaaaga tgccatctct ccgtgagtgg agtcggagac
      361 cggaacggaa aggggaacgt tatagactgc agaatcgatg gaagtgattt tcattatttt
      421 tcctctgatc gtaggcatgg gctataaggt tatgggttaa gggtcatagg aaaaaggggg
      481 cgcgccagag ccgcaaccaa atcggaggcg caaccccctg tggttgccca tcttgcgggc
      541 ggccacgggg gggccgcccc tacatttcag aaaaaatcat gacaaaattt aaagagtcta
      601 taccttattc atatagagca cagcgcagcc ctggccctta cgtggttcag ggcgcattct
      661 ccattatgtt ctttttagga ctttcccccc caattttccg gtatgctccc cgccttccac
      721 cgatagaact ccgttcacaa ttacctgccg aatcccgata ggatactgtt tgggatggtc
      781 ataggtggcc cggtcggtca ccgtggccgg attgaaaacc acaatatccg cagccttccc
      841 ctcggtcaag acgcctcgat ccttcaggcc gagacgtctg gcaggcaggg atgtcatctt
      901 tcggatggcc tcctccagcg atatgctctt tttctcccgg acatactttc ccaggatccg
      961 ggggaatgtc ccgtaaaccc gcggatgagg tcttccgccc aaaatgccgt ccgtgcagat
     1021 catccccaat ggatggcgca ggcccaggac cattttctcc tcatccatgc tgaaaaggat
     1081 catccccacc cgggcatctt ctgccaaaag aatgtcgaag gcaatctccg cagggtcctt
     1141 gccttccctc tcggcaatgt cggccaccga tcggcccagc cagtcccgat tctttttgcc
     1201 ctccgccgag gagatgatga tattctccca tcccacattg ttgacgaaat tatcccatcc
     1261 agggccttcc gggtcgggcg acggggggtt gatgaactgg tcccggattt tggcccggag
     1321 ggcggggtcc cgaagccgct ccagtgcctt ggccgggcca ccctccagga cccagggagg
     1381 aaggatggcc aggaacatgg tgcttcccgc ggtgtacgga tagatgtcaa aagtgatgtc
     1441 cagcccttcc cggtgcgccc gatccactgc ttccagggcc gtttccattt tgtgccaatt
     1501 ccgtttgccg gatgccttga agtgggagat gtgcagggga atctccgctt ctctggccag
     1561 gccgaccact tcttcaatgg cctccagcag gcggtcgctg ccgctgcgaa tatgaaccgc
     1621 taaaaaaccc cccatgcacc cgcaggtatg gagaagcctc agcagttcat cccgccgtga
     1681 aaaggtgcag ggcatgtaga ccatgcccag ggagattccg gctgcccctt cttccatggc
     1741 cttccgcact tcttcctcca tggcctgaac ctggaaaaca tccgggagta cattttccag
     1801 tccgagcacc accatgcgaa tatttccctg ggggaccaga aacgccaggt tggaaccggt
     1861 cggcttggcg gccagtcgtt cggcatagtc cccgatagac tcccagtccc aggggatcga
     1921 agggtctccg gtaagtccgg ataaatactg ccgccaagga gccacatacc cctttctgat
     1981 gggggcggcg gcaattccat cctgccccag aagctctgtg gtcactccct gcatgagctt
     2041 ggggagcaat gcaggttcgg ccaaggccat gaggtccgaa tggctgtggg tgtctatgaa
     2101 tcccggtgac accacggccc ccgccgcgga aaactcccgg gtcgcctccc ctttttcaac
     2161 ccggccgatg cgggctatcc ggtctccccg gatagccaca tccgcttttc ttcccggggt
     2221 cccggtccca tctacgaccg ttccccccag gatagctagg tcaaaaatga tggacctcct
     2281 tcaggaaaca agactgtggg cgtgaccggc ggctcggctg agctcgccga agttaggtcg
     2341 cccgcctttc ttcctctgta ttctctgcgg cctctgcgag agacgaatgt cttttatttt
     2401 gtcattcttc caaaacacga gataggatcc cccgagcctc ttctacgata ccttccaggt
     2461 gcgtttggct tttgaaactc tcggcataga gtttatagat gttctccgtc cccgagggcc
     2521 gggcggcaaa ccatccattc ggggttacca ccttcagacc gccgatggaa cccccattgc
     2581 ccggggctcg ggtcagtttg gccaggatgg gctcaccggc tagctccctt accgaaattg
     2641 cctctggagc cagccccttc agtcgacttt tctgctcggg agttgccgga gcgtcaatgc
     2701 gaaggtagta gggaactccc agccgggctg taagttcttg gtagtactcc cccgggtcct
     2761 ttcctgcccg ggcggtcatc tccgcagcca ggagccctag aagaattcca tccttgtccg
     2821 tgttccagac cgtcccatcc ttgcgcagaa agctcgctcc ggcgctttcc tccatgccga
     2881 agcagaggga acctttgagc agcccatcgg cataccactt gaacccgact ggaacttcga
     2941 agagcttcct tccgaacttc tccaccaccc ggtcgatcag gctgctgctg accagggttt
     3001 tgcccaccat tgacgccgca ggccacttgg atcgacggct caggaggtat gaaatggccg
     3061 ccgctaggta atgattggga ttcatgaggc ctgcggtccg ggtcacaatc ccgtgccggt
     3121 cagaatcggt gtcgtttccg aaggcaacct gaaactggtc tttgagttcc accagcccgg
     3181 ccatcgcata ggggctggag cagtccatcc ggattttccc atcgtggtcc agagtcatga
     3241 agccaaaggt cgggtcgatc cggggattca ccaccgtgag gttgagctga taatgctctt
     3301 tgataggctc ccaatacccg atcgaggacc cccccagggg gtccactccg atttggaccc
     3361 ctgccgagcg gataacctcc atgtccaaga cgtttgatag gtctttcaca tagggttcta
     3421 tgaaatccat ctcctgcgtc gtggatgcct tgatcgcctg gttgtaggaa accctcttga
     3481 cttcccggtt tgtggatcgc agcagagcat tcgcccggtc ctggatccaa ccggtcacgt
     3541 cggtatccgc cggacctccg ttggggggat tgtatttgaa acctccgtcg gcagggggat
     3601 tgtgagaggg ggtgatgacg atcccgtccc cgattccttc cttccggccc cggttgttga
     3661 tcaggatggc gtgggaaatt accggagtgg gagtataacc ctcatggctt tggatgaacg
     3721 ttgccacgtc gttggccgcc aggacttcga gggcggtctt ctgcgccgga ttggatatgg
     3781 cgtgggtatc cttgcccatg aacagggggc cgctgattcc ttttctgcgg cggaattcac
     3841 agatcgcctg ggtgatggcc aggatgtggg cctcgttgaa agacccctcc agcgggttcc
     3901 cccggtgacc gcttgtgccg aagcttactc tctggttggg gtcttcaatt tcaggccgtt
     3961 tatcataata ggctctctct agtcgggcga catcgatcag agcttcggga ggagccggct
     4021 tgccagccag gggatgcgtg ctcatcttgc tctcgccacc ctaccctttc cccatgacag
     4081 cttgcatcaa ggacctcacc ccctcctccg gtccctccgg gtgaccgaaa acggaggttc
     4141 cggcaaccag cacgcccgcg ccggcttcca ccacgcgggc ggcattttgg ggcgcgatcc
     4201 cgccatctac ctctatttca caattccggt ttctgcggtc cagaatctcc ctcaggaggc
     4261 ggaccttctt caggggatac tccatgaact tctgcccgcc gaaaccgggg ttgacggtca
     4321 tgaccagaat taagtcgagc ttctcaatga tctcttcgag catcccggcc ggggtgctgg
     4381 ggttcagggc tacgcccgcc ttcttcttta attctttgat cgcctggagg gtccggtcca
     4441 ggtgaaccga gttctcctgc tgcacgatga gcaggtccgc cccggccatg gcgaaggctt
     4501 caaggtaatt ctccggctgc tcgatcatga gatggacttc gaaaggaagc cgggttccgc
     4561 gccgcaggct cctcaccacc gggatgccca tggtgatgtt gggaacgaac cgcccgtcca
     4621 tcacatcgat gtgaaggcga tcggctccgg ctcgctcgac ggcctggatc tcttcgccca
     4681 gcttcagaaa atccgctgaa aggatggacg gtgcgatctt aatcatgttt cctcctgtga
     4741 ttcaccccct agacaccggg agcgcagagc ggcaaagctg aatttcctat aaaggttgga
     4801 taccaggatt ggtacaggca ttcccacttc gaccgccgcg gtaatcgtcc aggaccttct
     4861 ccggaatcga atcccttttc cattttatcg agggatgcat tctttaacct tgcacgagga
     4921 ttataacttt ccgggatatg ccggtcaagt aaggcgcggc ccttgcccac ccccagtttt
     4981 tcatgcttgt tggggttaag agtggaggaa tttggatcat caggctcggc gggtgcttat
     5041 ccttccatga aactcctccc catgttccgc aggcagaggc ctgagagggg atacctccaa
     5101 gcagaaaaaa gagccaaagc atttttccgt ctcaatcccc cccaggacag gcccgatcca
     5161 atggaagaac cactgcctca ggatgatgat ccaaatttct ccgccctgaa ccccatggga
     5221 tccccactcc tttccagagg tcttattttc gaaggactct ttttaagaac cgggggtggg
     5281 aagttgaaaa aagtcatgaa aaaagtcatt gacaagggaa ttaatttaat ctaaattctc
     5341 actgaattct taatccatcc aagccataat aattctctca taaac
//
LOCUS       DASPRR010000103        13712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1800101, whole genome shotgun sequence.
ACCESSION   DASPRR010000103 DASPRR010000000
VERSION     DASPRR010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13712)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800101"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /gene="rpiB"
                     /locus_tag="VEL68_03625"
     CDS             complement(<1..428)
                     /gene="rpiB"
                     /locus_tag="VEL68_03625"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HXZ35090.1"
                     /translation="MKIALGSDHAGFELKEDLRSFLAERQVEVLDLGAFGEAPVDYPD
                     VAIKVAGKVSRGEVERGLLICGTGIGMSIVANRFAGVRAALCHDPYTARISREHNDAN
                     LLALGGRLIGKGLAREILKVWLETEFQGGRHQKRLDKITGL"
     gene            complement(430..1674)
                     /gene="fabF"
                     /locus_tag="VEL68_03630"
     CDS             complement(430..1674)
                     /gene="fabF"
                     /locus_tag="VEL68_03630"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXZ35091.1"
                     /translation="MSSRRVVVTGLGMVSPLGTGVEKSWQALIQGKSGIGKITKFDTK
                     GFETTIAGEVKDFIPENFIDKKEMKRMDIFIQYAVAASVMAMEESGLRITPANADRVG
                     VVVGAGLGGLTTIESFHKVLLERGPGRISPFFIPMLIVNEAPGQISMRFGAKGPNTSA
                     VTACATGNHNIGDAWRMIQRGDADAIISGGVEATITPLAVSGFNAMKALSTRNDEPEK
                     ASRPFDKDRDGFVMGEGAGILILEELTRALDRGARIYAEIAGYGLTGDAYHITAPAPD
                     GDGATRCMDMALRSGNIAPEEINYINAHGTSTPINDPIETKAIKAVFGQHAGKLPISS
                     TKSMTGHLLGGAGGVEAIFTILTLYHGIIPPTINYETPDPECDLDYVPNKARNADVRV
                     AMTNSFGFGGTNAVLIFKKFEK"
     gene            complement(1776..2012)
                     /gene="acpP"
                     /locus_tag="VEL68_03635"
     CDS             complement(1776..2012)
                     /gene="acpP"
                     /locus_tag="VEL68_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXZ35092.1"
                     /translation="MEKTVEKRVIEIIVEQLKVSPEEVTLEASFIDDLGADSLDLVEL
                     IMAMEEEFGLEISDEDAEKIQTVQDAVNYISEHM"
     gene            complement(2124..2867)
                     /gene="fabG"
                     /locus_tag="VEL68_03640"
     CDS             complement(2124..2867)
                     /gene="fabG"
                     /locus_tag="VEL68_03640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXZ35093.1"
                     /translation="MGKLDGKVALVTGGAQGIGKAIALALAREGAQVAVSDINLEKAR
                     ETCKEIDPLGKKAVAIGGNVADAKAAEAMVEKTVEAFGGLDILVNNAGITRDGVLLRM
                     KEEDWDLVLAVNLKGAFQCTKTALRHLLKRKGGKIVNIASVTGQMGNAGQSNYAASKA
                     GLIGFTKSVAREYASRNIQVNAVAPGFIDTAMSQAIPQKEREMLLKLIPMERLGTAED
                     ISEAVLFLTSPASDYITGQVLNVNGGMFM"
     gene            complement(3253..4188)
                     /gene="fabD"
                     /locus_tag="VEL68_03645"
     CDS             complement(3253..4188)
                     /gene="fabD"
                     /locus_tag="VEL68_03645"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXZ35094.1"
                     /translation="MKKTALIFPGQGSQYVGMGKDFFDRSPAAREVFAEANEALGYDI
                     ANLCFQGPEEDLKLTANTQPAILATSIAALRVMQTETEVIPVAAAGHSLGEYGALVAA
                     GGLRFADAVRLVHLRGKFMQEAVPVGAGAMAAIMGMSTAEVEALCQEVARGEVLSPAN
                     FNSPGQIAIAGHAAAVQRAVEIVSKQKGKKAVLLPVSAPFHSPLMKPAGERLKEELDK
                     AEVGELHFPVLSNAEAHFYPGRESIRGLLVKQVDHPVRWEECMQELLATGADLVLEVG
                     PGKVLTGLMRRISKDTKMANVEDEASWEKAKELLK"
     gene            complement(4185..5339)
                     /locus_tag="VEL68_03650"
     CDS             complement(4185..5339)
                     /locus_tag="VEL68_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35095.1"
                     /translation="MDYFLSEDQRSIRDLARKIAEERVLPVRAELDEKEEFPWGPLKY
                     LAEAGLFGTYIPEEYGGLGFGSFENCLAIEEVSRVCIGVSVSFAASGLGSYPFLLFGS
                     EEQKKKYLPDVASGKRLAAFGLTEAGAGSDAGGIRTTARKDGNDYILNGTKQWITNGG
                     EAEIYSVIALTDKSKGPRGATAFILEKGMPGFDFGKKEKKLGIRCSATRELVFDDCRV
                     PRERVIGREGMGFIVAMRTLDRTRPGIGAQAVGLAQGAFEAAVAYARERHQFEKPIIS
                     FQAVQHILADMAAQIEAARALVYAVARWVDTNPKDISKYSAISKLFPSDVAMKVTVDA
                     VQIFGGYGFMRDYPVEKMMRDAKILQIYEGTNQVQRNVIGQALIKELASK"
     gene            complement(5570..6574)
                     /locus_tag="VEL68_03655"
     CDS             complement(5570..6574)
                     /locus_tag="VEL68_03655"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HXZ35096.1"
                     /translation="MRGNREINMIKPRIIATGSYVPPKVLTNAELEKMVDTTDEWITS
                     RSGIRERRIVERDVATSDLATQAALKALEMAGLPAEELDFIITGTNSPDMFFPCTSCF
                     LQSKIGAKKAAGFDILAGCTSFIHTLSLADKFIKENPKRKVMALGAEIMSKITDWKDR
                     GTCVLFGDGAGAVILAGEEGDRGVLSTHLHSDGSLWELLYMPGGGSANPPSHETVDKN
                     MHCIKMAGNQLFKVAVRALAEVSQETLEFNGLKSADIDYMIPHQANTRIIEAAAKMIN
                     FPMEKVYLNIDRYGNTSSATIPIALDEMNRSGMLKAGNLILLCSFGTGVTWGGALIRW
                     "
     gene            complement(6642..7700)
                     /gene="plsX"
                     /locus_tag="VEL68_03660"
     CDS             complement(6642..7700)
                     /gene="plsX"
                     /locus_tag="VEL68_03660"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXZ35097.1"
                     /translation="MKIAVDAMGGDFAPQNIVEGAVLAAREYGVSVVLVGDQEAVRKE
                     MARHPGSDSLPLTFVHASEAVGMDESPLTPIRKKKDSSIKVAYDLVKKGEAAALFSAG
                     NSGAVLATAVFVLEKVKGVDRPAIGTIFPTLKGWTLLLDAGANVDCKSFHLVQFAIMG
                     EAYAKYVLKIKKPAVGLLSNGEEESKGNELIRETNALLRKTSIGYVGPVEGRDIFNGR
                     ADVVVCDGFVGNAALKICEGLAEAIGAMIKEEVKGSLMAKLAYLLARGAVDDIKKKLD
                     YSEYGGAPLLGVDGVVIIGHGRSSPKAVKNAIRLAHEYAQIGLPKLLVQSLEENQEPQ
                     TQGGLAKVWEQIKEKIIS"
     gene            complement(7715..7897)
                     /gene="rpmF"
                     /locus_tag="VEL68_03665"
     CDS             complement(7715..7897)
                     /gene="rpmF"
                     /locus_tag="VEL68_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXZ35098.1"
                     /translation="MPNPKRRHSKTRRDKRRTHDRLASPQFSVCQNCGEVKAPHRVCP
                     HCGSYRGRTVIETEES"
     gene            complement(7916..8458)
                     /locus_tag="VEL68_03670"
     CDS             complement(7916..8458)
                     /locus_tag="VEL68_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXZ35099.1"
                     /translation="MIIQVEDVPAQGKQESFLEEEGPLNERLGGETTNLGLHFSGPIQ
                     VRLDLSRTGKILFIKSRVDAQVRCTCARCLDPFALTLSSEFKMSLRPRPDPRLAPSEE
                     IELSREDLETDFYDGEEVDLSPMIQDQVLLTLPPKVICREDCRGLCQRCGKNLNRETC
                     QCPGAEVDPRLAPLKHFRVH"
     gene            8614..8901
                     /locus_tag="VEL68_03675"
     CDS             8614..8901
                     /locus_tag="VEL68_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HXZ35100.1"
                     /translation="MEIKEFQNLIRQIYLERDQKRGADKTFLWLLEEVGELTRAYRRK
                     EDHVGTEMADVLAWMVSMANLLEIDLQAEVLKKYPRVCPLCSSNPCACPFR"
     gene            complement(9011..9496)
                     /locus_tag="VEL68_03680"
     CDS             complement(9011..9496)
                     /locus_tag="VEL68_03680"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HXZ35101.1"
                     /translation="MQRKFGRFGYFLFRGERSKSRGISISKKEFPVSRRAFEKMVKEA
                     IESIPPEMKERLENVAFLVRKNPSDLPDDWEEEDQGLLGLYHGISRKDRGVSYGNVLP
                     DRIVIYQRPLEKISSNLEELKENIRQTVIHEVGHYFGFDEDELRQLEEEGFQIQQGDE
                     P"
     gene            9589..10008
                     /locus_tag="VEL68_03685"
     CDS             9589..10008
                     /locus_tag="VEL68_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35102.1"
                     /translation="MKKILVLLIALIFALSLTGLAFAQAKPEKAAEPAKPAESAKPAE
                     VAKPAEPAKPAEAKKEEPKKEPPPKPVVYRLGGQVTALDAAAKKITIAQNSVKQQKKV
                     TLAVGKKAAQDLAGINVGDVVNVWVTGKSVTSIKKIF"
     gene            complement(10160..10858)
                     /locus_tag="VEL68_03690"
     CDS             complement(10160..10858)
                     /locus_tag="VEL68_03690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXZ35103.1"
                     /translation="MVDHPTNQICFFDMDRTLIDGNSGVSFMRYSLRRGKTTRWRVLK
                     SMIDYLRYRYDLLNMEKTYRDSLKPIAGVREGELIQFCKEWFEDVVRHRVYAEARDFV
                     RQHIDQGEKVAIISNATTYAVAPLAQYLGVPHVLATRLEVRQGVFTGEYIPPLCFRQG
                     KIFWAEKLARELGREVNQSTFYTDSITDLPLLERVKNPRIVNPDPKLRNLARKRGWPI
                     LDFAAPPGKKKKAS"
     gene            11077..11226
                     /gene="rpmH"
                     /locus_tag="VEL68_03695"
     CDS             11077..11226
                     /gene="rpmH"
                     /locus_tag="VEL68_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXZ35104.1"
                     /translation="MKRTYQPSNIRRKRTHGFRKRMSTKGGRLVLKARRAKGRKRLIV
                     KASES"
     gene            11261..11596
                     /gene="rnpA"
                     /locus_tag="VEL68_03700"
     CDS             11261..11596
                     /gene="rnpA"
                     /locus_tag="VEL68_03700"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXZ35105.1"
                     /translation="MALYTFRKEERIRKRADFQRALRGGARYQTPHFRISISPNGLSH
                     CRLGITAGKRIGSAVQRNRLRRRIREFYRQNKGRFPVSSDLLITAKEGAASLNFWQVS
                     EELKGLFRG"
     gene            11617..11871
                     /gene="yidD"
                     /locus_tag="VEL68_03705"
     CDS             11617..11871
                     /gene="yidD"
                     /locus_tag="VEL68_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXZ35106.1"
                     /translation="MLTQALLGLIRFYRYFLSPLTFPSCRFVPSCSAYAMEAVERFGP
                     AKGLLLAAWRILRCHPFHPGGYDPVVKNTRSVSPELSPRR"
     gene            11875..13542
                     /gene="yidC"
                     /locus_tag="VEL68_03710"
     CDS             11875..13542
                     /gene="yidC"
                     /locus_tag="VEL68_03710"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXZ35107.1"
                     /translation="MQIRALLAVIISLIILVIYQAYFAPPPAPPEKAAPEKAQTAAPA
                     KEEPVKAVSPGEKIPGITPPPQLSPTPAIKAEPGKDVAIETPLYTAVFNTRGARLKDF
                     RVKKYLDKIGEGAKPIDLATENLGSAYPFGVEMTHTNFPYSPDLLFQVNTETLKLGPD
                     RTQGELLFSWNSPGGIKVTQQMIFHADSYRIDINLQMANLSSQVVEGRPTLVWTGKLH
                     PHSGGGGMACIPGSGGSSTAAVPPFTALIKREFHEIELGDLKGGEKRFADQVQWGGFQ
                     DQYFLAAFIPQKMEGTELILKKISDTAGELRMAGPKASLSPGTQFSQSYLMFLGPKDY
                     DILKAFGQDLDKALDFGWFDIVAKPMLWAMKFSYQYVGNYGLAIIFLTIIIKIIFWYP
                     TQLSMRSMSEMKKLQPEMAKMREKYKDDKEKMNKELMELYRRYKVNPMSGCLPMAIQI
                     PVFFALYKVLLYSIELRHAPFYWWIQDLSAADPYYISPILMGGSMFVQQWMTPTTGDP
                     TQAKMMLIMPVVFTFMFLSFPTGLVIYWFFSNLLSIGQQLYMNRKNK"
BASE COUNT         2905 a         4036 c         3634 g         3137 t
ORIGIN      
        1 aggccggtga tcttgtcgag ccgcttctgg tgccggccgc cctggaattc agtctccagc
       61 cagaccttca gaatctccct cgccaatcct ttcccgatca gccgtccccc caaagccagg
      121 aggttggcgt cattgtgctc ccggctgatc cgggcggtgt acgggtcatg gcagagggca
      181 gcccggactc cggcaaagcg gttggccacg atggacatgc cgattcctgt accgcagatc
      241 agaaggcctc tttcgacctc tccccgggag accttccccg ccaccttaat ggccacatcc
      301 ggatagtcca ccggagcttc gccaaatgcg cccaggtcca aaacttcgac ctgtcgctcg
      361 gctaaaaagg atcgcaggtc ttccttgagc tcaaagccgg cgtggtcgct gcccagggca
      421 attttcatgt cacttctcga attttttaaa gatgaggacc gcattcgtgc cgccgaagcc
      481 gaaagagttg gtcatcgcca cgcgtacatc cgcgttccgg gccttgttgg gcacataatc
      541 caggtcgcac tcggggtccg gggtttcgta gttgatggtg ggggggataa ttccgtgata
      601 gagggtgaga atggtgaaga tcgcctccac ccctccggcc ccgcccagga gatggccggt
      661 catggatttg gtcgagctga tgggcagttt cccggcgtgt tgcccgaaaa cggctttgat
      721 ggccttggtc tcgatggggt cattgatcgg ggtggaagtc ccgtgggcgt tgatataatt
      781 tatctcttcg ggggctatgt tcccactgcg cagggccatg tccatgcatc gggtcgcgcc
      841 gtctccgtcc ggggccgggg cggtgatatg gtaggcatcc ccggtgagcc catagcccgc
      901 gatttcggcg tagattcttg ccccgcggtc gagggcccgg gtcaattctt ccaggatcaa
      961 aataccggct ccttcgccca tgacaaatcc gtcccggtcc ttgtcgaaag gacgggaggc
     1021 cttctccggt tcatcgttgc gggtggagag tgccttcatg gcgttgaaac cgctcaccgc
     1081 caggggggtg atcgttgctt ccacccctcc gctgatgatg gcgtccgcgt ctccccgctg
     1141 gatcatgcgc caggcgtccc cgatgttatg gttcccggtg gcgcaggcgg tgaccgccga
     1201 agtgttcggg cctttggccc cgaaacgcat ggagatctgg cccggtgcct cattcacgat
     1261 cagcatggga atgaagaaag gagatatccg cccgggccct ctttccagca gaaccttatg
     1321 gaaagattcg atggtggtca gtcctcccag tcccgcgccc acgacgaccc cgacccggtc
     1381 cgcattggcc ggggtaatcc tgagcccgga ctcttccatg gccatgaccg aagcagccac
     1441 ggcgtactgg atgaagatgt ccatccgctt catctctttt ttgtcgatga agttctccgg
     1501 gatgaaatct ttcacttccc cggcgatggt ggtctcgaat cccttggtgt cgaatttggt
     1561 gatctttccg attccggatt ttccctggat gagcgcctgc cagctctttt ccaccccggt
     1621 tcccaggggg gacaccattc ccaacccggt gaccaccact ctccgactcg acataaaacc
     1681 tcccgattca accgagaagg ccctttcccc tctggctttg cctccttccc gccgcggcga
     1741 aaatccaccg tttcggggga aagccagccc tctccttaca tatgttcgct gatataattc
     1801 accgcgtcct gcacggtctg gatcttttcg gcatcctcat cggaaatttc cagaccaaac
     1861 tcttcttcca tggccatgat caactcgacc aggtccagcg aatccgctcc caggtcatca
     1921 ataaaggagg cctccagggt cacttcttcc gggctgacct tgagctgctc cacgataatt
     1981 tcgatgactc ttttttccac tgttttttcc acaatcccac ctcctgacga aactttttat
     2041 gattttcccg atggatgaat ctttttactt tctttatccc cccctgtcaa gggaatttca
     2101 aggggaattc ggcctggggt acgctacatg aacatacccc cgttgacgtt taacacttgc
     2161 ccggtaatat agtcggaggc cgggctggtc aggaagagga cggcctcaga gatgtcctcg
     2221 gcagtcccca gccgttccat gggaatcaat ttcaggagca tctctcgctc tttctggggg
     2281 atggcctgac tcatggctgt gtcgatgaac ccgggagcca cggcatttac ctgaatgttc
     2341 cggctggcat actcccgggc cacggatttc gtgaagccga tcaaccccgc cttggaggcg
     2401 gcgtagttgg actggccggc gttgcccatc tggccggtga ccgaggcaat gttgacgatc
     2461 ttgcctccct tccgtttgag aagatggcgc agggcggtct tggtgcattg aaaggcgccc
     2521 ttcaggttca cggccaggac caggtcccag tcctcctctt tcatccgcag aagcaccccg
     2581 tcgcgggtga ttccggcgtt attcaccagg atgtccagac ccccgaaggc ttccacagtc
     2641 ttttccacca tggcctccgc agccttggcg tcggcgacgt ttccgccgat ggccaccgcc
     2701 ttctttccca ggggatcaat ctccttgcag gtctcccggg ccttttccag gttgatatcg
     2761 gacaccgcca cctgggcccc ttcccgggcc agagccagag cgatcgcttt cccgatcccc
     2821 tgggcccccc cggtcacaag agcgactttg ccatccaatt tacccatgat ctttctccga
     2881 ttttttcaac ccgcatgtta ctaaggagtc cataattgaa aagacacatt tttctaagaa
     2941 tgttccctaa ccccgatcct cctcacttcg cctgtcaccg ttcaccctga gcttgtcgaa
     3001 gggtgcgtcg taatgccgtt catgcttcga caagctcagc acgaacggct ttcccctcag
     3061 cgtccacaca attatggact gattagtaaa tcccaaattc caatccccag atctcaaata
     3121 aaaaccgaat ctcaatgttc caataaccaa acgtttggaa ttttcatgtt tccatgattg
     3181 gaatttgttt ggtgcttggt gtttggaatt tggaatttat tcgaatccgt ttggatccgc
     3241 gtaccattcc atctatttca atagctcttt ggccttttcc cagctagcct cgtcctccac
     3301 gttggccatc ttggtgtcct tgctgatccg gcgcatgaga ccggtgagga cctttcccgg
     3361 ccccacctcc agcaccaggt cggctcccgt ggccaacagc tcctgcatgc attcctccca
     3421 gcggactgga tgatccacct gtttgaccag aagtccccgg atgctttccc ttcccgggta
     3481 gaaatgggct tcggcgttgg aaaggacggg gaaatgaagc tctcccacct cggccttgtc
     3541 cagctcctct ttcaaccgtt cgccggcagg cttcatcagc ggactgtgga aaggggcact
     3601 caccggcaga agaacagctt tctttccctt ctgtttggaa acgatctcca cggccctctg
     3661 cacggctgcg gcgtggccgg cgatcgcaat ctgtccgggg gagttgaaat tggcagggga
     3721 aagaacctcc ccccgggcca cctcctggca aagggcttcc acctcagcgg tggacattcc
     3781 catgattgcg gccatggccc ccgcccctac ggggaccgct tcctgcatga acttccctcg
     3841 caggtgaacc aggcgaacgg catcggcaaa gcgcagcccc cccgcagcca ccagagcgcc
     3901 gtactctccc agactgtgcc ccgcggcggc gacggggatg acctctgttt cggtttgcat
     3961 cacccgcagg gcggcgatgg aagttgccag aatcgccggc tgggtgttgg cagtgagctt
     4021 caggtcttct tccggcccct ggaaacaaag gttggcgata tcgtacccca gggcttcatt
     4081 ggcttccgca aacacctccc gggcggccgg ggaccggtca aaaaaatctt tccccattcc
     4141 cacgtactgc gatccctgtc ccggaaaaat caaggccgtc ttcttcattt actggccaac
     4201 tccttgatca gcgcctggcc gatcacgttc cgctgaacct ggttggttcc ttcgtagatc
     4261 tgcaggatct tcgcgtcccg catcattttt tccaccgggt aatcccgcat gaatccgtac
     4321 cccccaaaga tttggacagc atcgacggtg actttcatgg ccacgtcgct ggggaagagc
     4381 ttggagatgg ccgagtactt ggaaatatcc ttgggattgg tgtccaccca gcgggccacg
     4441 gcatagacca gagcccgggc cgcttcgatc tgcgcagcca tgtcggccag gatatgctgg
     4501 acggcctgga aggagataat cggtttctca aactggtggc gctcccgggc gtaggccacg
     4561 gcggcctcaa aagccccctg ggccaatcct acggcctggg cgccgattcc cgggcgggtc
     4621 ctgtccagag tgcgcatggc cacgatgaag cccattcctt cgcggccgat gactctttcc
     4681 ctggggacgc ggcagtcatc aaagaccaac tcccgggtcg ccgaacagcg gatacccagc
     4741 ttcttttcct tcttcccgaa gtcgaacccc ggcatcccct tctccaagat gaaggcggtg
     4801 gctccccgcg gccctttgct cttatccgta agggcgatga ccgaataaat ctcagcctcc
     4861 cccccgttgg taatccactg cttggtccca ttgaggatgt aatcattccc gtcctttcgg
     4921 gcggtagtcc ggattccgcc ggcatcgctt ccggcgcccg cttcggtgag gccgaaggcc
     4981 gccagtcttt tcccagaggc cacgtcgggg agatattttt tcttctgctc ctcactcccg
     5041 aagagcagaa aggggtaaga ccccaggccg gaggcggcga aggatacgga gaccccgatg
     5101 cagacccggg agacttcttc gatggccaag cagttctcaa aggacccgaa tcccagcccg
     5161 ccgtactcct cggggatata cgttccgaac aggccggctt cggccaagta cttcaggggt
     5221 ccccagggga attcttcctt ttcgtccagc tcggcccgca ccggaagcac ccgctcctcc
     5281 gcaattttcc gcgccaagtc ccggatggac ctttgatctt cgctcagaaa ataatccatg
     5341 cgtcgtattt ctccttatct cctaaaatgg gacgcagatt cccgcagaaa aaagcggata
     5401 aaatactttt caaacaaatg gctaacgaca gagaatgagt gagaacggga ggggacgcgg
     5461 atcaaccagg aaaaacccgg gaaaaaacat aacctacaac ccgtgttcct ccgcgtcttt
     5521 tcctctcaca tcttgtgaat ctgcgtgaat ccgcgttcca ttcgggtttt caccagcgga
     5581 tcagggctcc tccccaggtg acgccggtgc cgaaagaaca caggaggatc aggttgccgg
     5641 ctttcagcat cccggaccgg ttcatctcgt ccagggcgat gggaatagtg gccgaagacg
     5701 tattcccgta gcgatcaatg ttgaggtaca ccttttccat ggggaagttg atcattttgg
     5761 cagcggcctc aatgatccgg gtgttggcct ggtgggggat catgtagtcg atatccgcgc
     5821 tcttcaggcc gttgaactcc agggtctcct gggacacctc cgccagcgcc cgcaccgcca
     5881 ccttgaagag ctggtttccc gccatcttga tgcagtgcat attcttgtcg acggtttcat
     5941 gggaaggagg attggccgat ccccccccgg gcatgtagag aagctcccag agggagccgt
     6001 ccgaatggag gtgggtggaa aggacccccc ggtccccttc ctctcccgcc aggatgaccg
     6061 ccccagcccc gtctccgaag agaacgcagg tgccccgatc cttccagtcg gtgatcttgg
     6121 acatgatctc ggcgcccagc gccatgacct tccgcttcgg gttttctttg atgaacttgt
     6181 ccgccaggga aagggtgtga ataaagctgg tgcaccctgc caggatgtcg aagcccgccg
     6241 cttttttggc cccgattttg gactggagga aacagctggt gcaggggaaa aacatatccg
     6301 ggctgttggt cccggtaatg atgaaatcga gttcctccgc cggaaggccg gccatctcca
     6361 gagccttcag ggcggcctgg gtggccaaat ccgaggtggc cacatccctc tccacgatcc
     6421 tccgttcccg gattccggag cggctggtga tccattcgtc ggtggtatcc accatttttt
     6481 ccagctcggc gttggtcagg actttgggag ggacgtaaga accagtggcg atgatgcgcg
     6541 gcttgatcat gttgatttct ctgttccccc tcatagaccc ctttcccccc tgctttctcc
     6601 cgggtaggga agaaggcaaa ggagcctatg gaaagacccc ttcagctgat aatcttttct
     6661 ttgatctgtt cccagacctt ggctaggccg ccctgtgttt ggggttcctg gttttcctcc
     6721 aggctttgga cgaggagctt gggaaggccg atctgggcgt actcgtgcgc cagccggatc
     6781 gcattcttga cggccttggg cgaggaacgg ccgtggccga tgatcaccac accatcgact
     6841 cccaggaggg gtgccccccc gtattcagaa tagtccagct tcttcttgat gtcgtcgacc
     6901 gcaccgcggg cgagaagata cgccagcttg gccatgagac tgcccttgac ctcttccttg
     6961 atcatggccc cgatggcttc ggctaacccc tcgcagatct tcagggctgc attccccaca
     7021 aagccgtcgc agaccaccac atcggcgcgc ccgttgaata tgtcgcgccc ttccaccgga
     7081 cccacatacc cgatcgaagt tttgcgcagg agggcgttcg tttcccggat aagctcgttg
     7141 cccttggatt cttcttcccc gttgctgagc aggcctaccg caggtttttt gatcttcagg
     7201 acgtatttgg cataggcttc gcccatgatg gcaaattgaa ccaggtggaa ggatttgcaa
     7261 tccacgttgg ccccggcgtc gagcaggagg gtccagccct tcagggtcgg aaagatcgtc
     7321 ccgatggccg gcctgtccac ccctttcact ttttccagga cgaagaccgc tgtggccagg
     7381 actgccccgg aattccccgc gctgaagagc gcggcggcct ctcccttctt gaccaggtca
     7441 taggcgactt tgatggaaga atccttcttc ttgcgaatcg gggtcagggg agattcatcc
     7501 atgcccaccg cttcggaggc atggacgaac gtcaggggaa gggaatcgct gcccggatgc
     7561 cgagccatct ctttccgcac ggcctcttgg tcccccacca gtaccacgga aaccccgtat
     7621 tcgcgggcgg cgagaaccgc cccctcgacg atgttttggg gggcgaagtc cccgcccatg
     7681 gcgtccacag cgattttcat cgttcggagt tccttcagct ttcttcggtc tcgatcaccg
     7741 tccgccctcg gtagcttccg cagtgggggc agacccggtg aggcgccttg acttccccgc
     7801 aattctggca gacggaaaat tggggagagg ctagccgatc gtgcgtccgt cgcttgtccc
     7861 gtctggtttt tgaatgtctt cttttagggt tgggcatata ccaatcacct cgccatcaat
     7921 gaacccggaa atgtttcaag ggcgcgagcc tggggtcaac ttccgcgccg ggacactggc
     7981 aggtctcccg gttcaggttt ttcccgcacc gttgacataa cccgcgacaa tcctcccggc
     8041 agatgacctt ggggggaaga gtcagcaaaa cttggtcctg aatcatgggg cttaaatcga
     8101 cctcttcccc atcgtaaaaa tcggtctcca ggtcttcccg gctgagttcg atctcctccg
     8161 aaggagcgag tctggggtcg ggcctgggtc ttaagctcat tttaaattct gaggaaagag
     8221 tcagggcgaa agggtcgagg cagcgggcgc aagtgcatct gacctgagca tcgacccggc
     8281 tcttaatgaa gagaatcttt ccggtacggg aaaggtccaa ccgaacttga atcggacccg
     8341 aaaaatgaag gcccaaattg gtggtttctc cccccaaacg ctcattcagg ggtccttcct
     8401 cttcaagaaa agactcctgc ttcccctgag cgggtacgtc ttcgacttga atgatcaagg
     8461 gtacccctga gctgcgaatc gaatataaaa atcctaatat ataatgaaaa acctctttgt
     8521 caagaaaaat atctccctaa aaagcttgtt ttacccccta ggtcatggta atgaataatc
     8581 atttttatat ctcccggacg gagggaaaaa cccttggaaa tcaaagaatt tcagaacctg
     8641 atcaggcaaa tatacttgga aagggaccaa aaacgaggag ctgataaaac cttcctctgg
     8701 ctccttgagg aagtggggga gctcactcgg gcttaccgca gaaaagaaga ccatgtgggg
     8761 acggaaatgg ccgatgtcct ggcctggatg gtttctatgg ctaaccttct ggaaatcgac
     8821 ttgcaagccg aggttctgaa gaaatatccc cgggtatgcc ccctgtgttc ctccaaccct
     8881 tgcgcctgcc cgttccggta gatggcggcc tgaacgatct aggaataaga aaactccata
     8941 gacccgtatt ttcgaatgaa agcagcatgg gttgggaagg ctcgttcttc attttgaccc
     9001 ttcgaatttc ctacggttca tctccctgtt gaatttggaa tccttcctcc tccagttgcc
     9061 gtagctcatc ctcatcaaaa ccgaaatagt gccccacttc atggatcacc gtctggcgga
     9121 tattttcctt caattcttca aggttggaac tgatcttttc aaggggcctt tggtagatga
     9181 caattcggtc cggaagcacg ttgccatagc tgacccctcg atctttgcgg gagattccgt
     9241 ggtaaaggcc gagcagacct tgatcctctt cctcccagtc atcggggagg tccgagggat
     9301 tctttcggac aaggaaagcc acattctcca gccgttcctt catttccggg gggatgcttt
     9361 ctatggcctc tttcaccatc ttttcgaaag cccgtctgga gacgggaaac tcctttttag
     9421 aaatggaaat cccccttgat tttgatctct ccccccgaaa gaggaaatac ccaaatcgtc
     9481 caaatttccg ttgcattatt ctcatcctta ttgtattctt gcaatgaagt tttgaaaagg
     9541 atatatttat ccttggcaat acctaaaaaa acaaggaggt aaagtaggat gaaaaagatt
     9601 ttggtgctcc tcatcgcgct aatctttgct ctttccttga ccggcctggc gtttgcccag
     9661 gctaaacctg aaaaggcagc cgaaccggcc aaaccagccg aatccgctaa accagcggaa
     9721 gtcgccaaac cggccgaacc cgcgaaaccg gcggaagcaa agaaagaaga gcccaagaag
     9781 gaacctcctc ccaaacccgt tgtgtaccgg ctgggcggac aggtaactgc cctggatgca
     9841 gcggccaaaa agattaccat tgctcagaat tcggtgaagc agcagaagaa ggtcaccctg
     9901 gcggtaggga agaaagccgc tcaggacctg gctggaatca acgtggggga tgtggtcaat
     9961 gtgtgggtga ccggcaagag cgtaacctcg atcaaaaaaa tcttctgaac agaacctata
    10021 aattcgcccg tttttgacgg acaggggagg atgaaaggat tttcccaaaa accggatcgc
    10081 ctcccatggg gtggaggaag cctgctcgca aggttctagg ttccttctcc gtagcgcccg
    10141 ctcaaaagaa ggcatcccat caggatgcct tctttttttt cccgggaggg gccgcaaaat
    10201 ccaggatggg ccagcctctc ttcctggcaa gattccggag tttcgggtcc ggattcacga
    10261 ttctgggatt tttgaccctt tccaggagag gaaggtccgt gatgctgtct gtgtagaagg
    10321 tgctctggtt gacctccctt cccaattccc gggccagttt ttccgcccag aatatcttcc
    10381 cctggcgaaa gcagagagga ggtatgtatt ctccggtgaa gaccccctgc cggacttcga
    10441 gtcgggtagc caaaacatga gggaccccca ggtattgagc caggggggct acggcataag
    10501 tggtggcatt ggagatgatg gccaccttct ccccctgatc aatatgctgg cgtacaaaat
    10561 cccgggcttc ggcgtaaacc cgatggcgga ccacatcttc gaaccattcc ttgcagaact
    10621 gaatgagttc tccttcccgg actcccgcta tcggcttcag cgaatcccgg taggttttct
    10681 ccatattcag gaggtcgtac cggtatcgga ggtaatcgat catcgattta agaaccctcc
    10741 atcgcgtggt ctttccccgg cgcaaggaat agcgcataaa ggaaaccccc gagttgccat
    10801 cgatcagggt cctgtccata tcgaaaaaac agatttgatt tgtgggatga tcgaccataa
    10861 tcaaaaaaga tgatccgcaa agcctggcgg aaagatccta gggataaacg ccctcagagg
    10921 gcttcttcaa cctattttaa acttgcggtt tgaaaatgaa aagaatataa tattttttta
    10981 aataaaatct gaattttcct tcgctccaaa ttcaggacaa gagcctgaat tcaataaaaa
    11041 tttctaagat aaaaaacgac aaacaggggt gcatgggtga agagaactta tcagcctagc
    11101 aatattcggc ggaaaagaac ccacgggttc agaaagcgga tgtcaaccaa gggtgggaga
    11161 ctggtcctca aggcgaggag ggcgaaggga agaaagagac tgatcgtcaa ggcgagcgaa
    11221 tcctagaacc gattttcatt agaaatcgga gccctggaag atggccctgt ataccttccg
    11281 caaagaggag aggattcgta aacgggccga ctttcagcgg gccttaagag gaggagctag
    11341 atatcaaacc ccccattttc ggatttccat cagccccaac gggctttccc attgcaggct
    11401 ggggattacg gcggggaaaa ggatcgggtc agcggttcag cgcaatcgtc tcaggcgccg
    11461 gattcgtgag ttttatcgcc agaacaaagg ccggtttccg gtttcttcgg acctgttgat
    11521 taccgccaaa gagggcgccg caagcctgaa tttttggcaa gtatcggaag aactgaaagg
    11581 attgtttcgg gggtgatggc ccccttttga ttcaggatgt tgacccaagc ccttttaggt
    11641 ctcattcgat tttatcgtta ttttctctcc ccgctgacct ttccctcttg tcgctttgtg
    11701 ccctcatgct ctgcctatgc catggaggca gtggagcggt tcggcccggc caaggggctg
    11761 ctcctggcag cttggagaat cctgaggtgc cacccctttc atcccggcgg ctacgatccc
    11821 gtggtgaaga acacccgatc ggtcagcccg gagttaagcc cacggaggta atctatgcag
    11881 attcgggccc ttttggcagt cattatttca ttgattattc tggtcattta tcaggcctat
    11941 ttcgctcccc cccctgctcc tccagaaaaa gcggctccgg aaaaagccca gaccgcagct
    12001 ccggccaagg aggaaccggt gaaggccgtt tcccccggag aaaaaatccc cgggattacc
    12061 cccccgcccc agctttcccc cactccggct atcaaagccg aaccgggtaa agatgtagcc
    12121 atcgagactc ctctctacac ggcggttttc aacacccggg gggcccgcct gaaggatttc
    12181 cgggtcaaga aatacctcga taaaatcggc gagggggcca agccgattga tctggccaca
    12241 gagaacctgg gaagcgccta tcctttcggc gtggaaatga cccacaccaa tttcccttat
    12301 tccccggacc ttctcttcca ggttaatacc gaaactctca aactgggccc ggaccgaacc
    12361 cagggagaac tcctgtttag ctggaattcc cccggaggga tcaaggtcac ccagcagatg
    12421 atcttccatg ccgacagcta ccggatcgac atcaacctcc agatggccaa tctttcctcc
    12481 caggtcgtgg agggaaggcc gaccctggtg tggactggca agctccatcc tcactccggc
    12541 gggggaggca tggcttgtat ccccgggtca ggcgggagtt cgacggcggc cgttcctccc
    12601 ttcacggcgc tgatcaaaag ggaatttcat gaaatcgagt tgggcgacct gaagggcgga
    12661 gagaagcgtt tcgccgacca agtccaatgg gggggatttc aggatcagta tttcctggcc
    12721 gctttcatcc ctcaaaagat ggaaggcacc gagctcattt tgaagaaaat ctccgacacc
    12781 gccggcgaac tgcgcatggc cggccccaag gcctctcttt ccccgggaac ccagttctcc
    12841 cagtcctatc tcatgttcct aggcccgaag gactacgata tcctcaaggc cttcgggcaa
    12901 gacctggaca aagcgctgga cttcgggtgg ttcgacatcg tggccaagcc catgctctgg
    12961 gccatgaaat tctcctacca gtacgtgggg aactacggcc tggccattat ctttctcacc
    13021 atcatcatca agatcatctt ctggtacccg acccagctca gcatgcgctc tatgtcggag
    13081 atgaagaagc tccagccgga gatggccaag atgcgggaga aatacaagga cgataaggag
    13141 aagatgaaca aggagctcat ggagctctac cgccgctaca aggtgaatcc catgagcggc
    13201 tgtctgccca tggccatcca aattccggtc ttcttcgccc tatacaaggt cctgctctac
    13261 tccatcgagc tgcgacatgc ccccttttac tggtggatcc aagacctttc ggccgcggac
    13321 ccctattaca tctctcccat tctcatgggg ggatccatgt tcgttcagca atggatgacc
    13381 cccaccaccg gggaccccac ccaggccaag atgatgctga ttatgccggt cgtctttacc
    13441 ttcatgttct tatcctttcc aacgggcctg gtcatttact ggttcttcag caacctgctc
    13501 tccatcgggc agcagctcta catgaaccgg aaaaacaaat aagaggcgag ggaacccatg
    13561 aagtctgtcg aaactgaggg caaaagcacg gaggaagcga ttcataaagc ctgcgaagaa
    13621 ctgggagctc cccggcaaga gttggagatc gaggtccttt ccaacggttc cagcggattc
    13681 ctgggaatgg gggccaagaa tgccagaatc cg
//
LOCUS       DASPRR010000104         4822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1960729, whole genome shotgun sequence.
ACCESSION   DASPRR010000104 DASPRR010000000
VERSION     DASPRR010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960729"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VEL68_03715"
     CDS             complement(<1..633)
                     /locus_tag="VEL68_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35108.1"
                     /translation="MKKSLVLMSVGILVLAFLGTSAFAQQKMVLKKYPEIKLGFTTTN
                     FLKPLPVSLDNKKKLLDLASELGCSWVEIRDPSASLIVDECKQLMAYAKSKNIELGYA
                     LQVGLLDPTFSEIFTRGLPNAAVFEGPRTIRSLAAGPEFDKDPKKTTWTLSELFKAVQ
                     VANRSANLARAAGLKYVVENSFQALKGDGATGFGMTEFAANVNSNLFFQCD"
     gene            complement(821..2257)
                     /locus_tag="VEL68_03720"
     CDS             complement(821..2257)
                     /locus_tag="VEL68_03720"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ35109.1"
                     /translation="MGIRTMKEYKESLRDGRRVYIRGEKVDDVTRHPVLGVTADTVAQ
                     GFALTSHPDPEVRNLFTAPHPETGELINRFFVTPRNPEDLANRTKMIQRSIELTGGLP
                     FGKDIGTDCLNAAFVVAGMMGNKQYQENARNFLEYVRKSDLAMCGAVTCVKGDRSQEP
                     SKQKHPDYYLHVVDKKKDGIVVKGAKIHITSAPVANEIIVVPTRQMRESEGDYAVSFA
                     IPANTKGVVFICRGGRGAWTEHEFHPDRPIRELTEAMIVFDDVFVPWERVFMCGEWKF
                     SMLLAYTFATFHRFTAISYKVPAVEVMAGIAAAMAEYNGLDKVGHIRDKLAEIAGYVE
                     TLRALANASAKDPVMYGDIAVPNPLITNMAKLHFASKYHEIMKLVQDICGGIIATTPD
                     KKDWDNPDIHAYLEHYLGGSAKFSTEDRLRMIHLAQRMACSHESAFHEVTTVHAEGSM
                     ATQMMMIMAEAPFKRYKTMARAAAGIKI"
     gene            2372..2887
                     /locus_tag="VEL68_03725"
     CDS             2372..2887
                     /locus_tag="VEL68_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXZ35110.1"
                     /translation="MNPAVPQSYQEIFSLIGQFSRALRCCQQEETFCLDLTLSQFFIL
                     HSIAGKRIIQLAELHGILSVEKSTTTRMVSPLIRRGLVVQEKSEHDSRALNLRLTPQG
                     EETYGRIWACLTDFLDTIQKGIPAGERKKIYGATRAFLDAVQGACKARSTRKMVPKGE
                     GNGCCRRNAQG"
     gene            2895..4022
                     /gene="hgcA"
                     /locus_tag="VEL68_03730"
     CDS             2895..4022
                     /gene="hgcA"
                     /locus_tag="VEL68_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_252989023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2937..2939,aa:Sec)
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HXZ35111.1"
                     /translation="MIEPLPLAPPAGSCUPPKPQAQVGLPRLDQGFVVGSVETPVGKI
                     PQVSSSLRWEDQKGAWKVRWGIGRMTYTIDPGLYALENPDRSSPVLVTANYKLTFDRL
                     RRALPGKNFWVLALDTKGVNVWCAAGKGTFGTEELVERIKLSGLEKVVDHRELILPQL
                     GAPGVAAHQVRKSSGFKVIYGPILAKDLPAFLEAGKKATPEMRRKAFTAWERAVLIPI
                     ELVHVLKWMVILLPALFLLGGLAGGGTFWQNALTHGVLAAAALILSLIMGAVLTPLLL
                     PFLPGRAFALKGFFLSILGVIFLLVVFGGGEMNWPGILERLAWVLLVPALTAFLAMNF
                     TGASTYTSLSGVKKEMRWALPLEIGGAALGLVFWLGALFYL"
     gene            4188..4520
                     /gene="hgcB"
                     /locus_tag="VEL68_03735"
     CDS             4188..4520
                     /gene="hgcB"
                     /locus_tag="VEL68_03735"
                     /inference="COORDINATES: protein motif:HMM:NF040864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HXZ35112.1"
                     /translation="MNRMVYLKNVATLRVFEDKCVRCGMCLEVCPHAVLSLNGGKVKI
                     EDRDACMECGACAQNCPAGAIVVKSGVGCANAVINSMLGRKSDSCCCVIEEKGTASEA
                     CTDNKVCC"
     gene            complement(4637..>4822)
                     /locus_tag="VEL68_03740"
     CDS             complement(4637..>4822)
                     /locus_tag="VEL68_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35113.1"
                     /translation="VLEPAYNFAGIETRPIGENIWTVALFLGFYLLYTLLYGFSILFL
                     FEGLGIQATKSKGKKEA"
BASE COUNT         1030 a         1283 c         1370 g         1139 t
ORIGIN      
        1 gtcacattgg aagaacaggt tggagttcac gttggcggcg aattccgtca tgccgaaccc
       61 ggtagcaccg tcccctttca gggcttggaa agaattctcc accacgtatt ttagacccgc
      121 tgcgcgggcc aggttggctg accggttggc cacttggacg gctttgaata attctgacag
      181 ggtccaggtc gtctttttag ggtctttgtc gaactccggt ccagcggcca ggctccgaat
      241 cgtccgggga ccctcaaaca cagccgcatt gggcagccca cgggtgaaga tttccgagaa
      301 agtcgggtcc agaagaccga cctgcagggc atagccgagc tcgatattct tggatttggc
      361 ataagccatg agctgcttgc attcatcaac gataagggaa gcgctggggt cccgaatttc
      421 aacccaggag cagcccaatt cgctggccaa gtccaatagc tttttcttgt tgtccaggga
      481 aacaggaagg ggttttaaga agttcgtggt ggtaaatcca agcttgatct ccggatattt
      541 ctttaaaacc attttttgct gcgcgaaggc actcgtaccc aggaaagcca ggacaagaat
      601 tcccaccgac ataagaacca aactcttctt catgatgact cctcctttcg gggttaaagg
      661 gtactgcgaa tccttcgttt cccttgattc gaaggtaagc ccagcaccta aatcacaaac
      721 cgagaccgga tagtatttta acagcgccaa acggcctgtc aagcccttcg tttccgcggc
      781 cccgttcagc cgggcgcctg cgcccggctg aacaagatgt tcagattttt atccctgccg
      841 cggccctggc catggtcttg taccttttga acggagcttc ggccatgatc atcatcatct
      901 gggtggccat ggagccctcg gcatgaaccg tggtcacctc atgaaaggcc gattcgtggg
      961 agcaggccat ccgttgggcc aggtggatca tgcgcagccg gtcttcggtg gagaatttcg
     1021 ccgagccccc caggtaatgc tccaggtaag catggatatc ggggttgtcc cagtcctttt
     1081 tgtcgggggt ggtagcgatg attcccccgc atatatcctg tacgagtttc atgatttcat
     1141 ggtatttgct ggcgaaatgg agcttagcca tattggtgat gagcgggttg gggacggcaa
     1201 tgtccccgta catgacggga tccttggccg aggcattggc cagggcccgg agcgtctcca
     1261 cgtatccggc gatctcggcc agcttgtccc ggatatgtcc cactttgtcc agcccgttgt
     1321 attcggccat ggcggcggcg ataccggcca tgacctccac cgccggaact ttgtaactga
     1381 tggccgtaaa gcggtgaaag gtggcgaaag tgtaagccag gagcatggag aatttccact
     1441 ctccgcacat aaagacccgt tcccagggga cgaagacgtc atcgaacacg atcatcgcct
     1501 cggtaagctc ccggatgggc cgatccggat ggaactcgtg ctcggtccag gctccccgac
     1561 ctccccggca gatgaagacc acccccttgg tgttcgccgg aatggcaaag ctcaccgcat
     1621 agtcgccttc gctttcccgc atttgcctgg tgggcaccac gatgatctcg ttagccaccg
     1681 gcgcactggt gatatgaatc ttggccccct tgaccacgat cccatctttc tttttatcca
     1741 ccacgtgaag gtaataatct ggatgcttct gcttggaggg ttcttgcgac cggtctcctt
     1801 tgacacaagt tacggctccg cacatggcca gatcgctctt gcggacgtat tccaagaaat
     1861 ttcgggcatt ttcctggtat tgcttgttgc ccatcatgcc ggcgacgacg aacgccgcgt
     1921 taaggcagtc agttccgatg tctttcccga aggggaggcc gccggtcagt tcgatggagc
     1981 gctggatcat cttggtgcgg ttggctaagt cctccgggtt ccggggcgtt acgaaaaagc
     2041 ggttgatcag ctctcccgtc tcaggatggg gggcggtaaa taagttccgt acttccgggt
     2101 cggggtggga ggttagagca aatccctggg ctactgtatc ggcggtgact ccaaggaccg
     2161 gatggcgggt gacgtcatcc accttttccc cccggatata aacccggcgc ccgtctctca
     2221 aactctcttt atattccttc atcgtccgaa ttcccatggc ccacctcctg agatcatttc
     2281 tggaaaggtc aacttcattt agaccatttg gagaattttt acaataaaaa aatgtcttga
     2341 cgggcccgat atatagttgt agtatacaac catgaatccg gccgtgcctc aatcgtacca
     2401 agaaatcttt tccctcatcg ggcagttttc ccgggccctg cgatgctgtc agcaggagga
     2461 aactttttgc ctggatttga ctctttctca attcttcatc cttcacagta ttgccgggaa
     2521 aagaatcatt caactggcgg aactccacgg tatcctgtcg gtggaaaaga gcaccaccac
     2581 ccggatggtc agcccgctca tccggagggg attggtcgtc caggaaaagt ccgaacatga
     2641 ttcccgggct ttgaatctcc gtttgacccc tcagggggaa gagacatacg gaagaatctg
     2701 ggcctgtctg accgacttcc tggataccat ccagaaagga attccggcag gcgaaagaaa
     2761 gaaaatctac ggggcgaccc gggctttctt ggacgccgtg cagggcgcct gtaaagcccg
     2821 ctcaacccga aagatggttc cgaaaggaga aggaaatggc tgttgccgaa gaaatgccca
     2881 gggatagcct cccgatgatc gaacccttac ccttggctcc ccctgccggg agctgctgac
     2941 cccccaaacc tcaggcccag gtgggcctgc cgagattgga tcagggtttt gtcgtgggtt
     3001 ccgtggagac cccggttggg aaaatccccc aggtttcctc ctcgctgaga tgggaagacc
     3061 aaaagggggc ctggaaggtg cggtggggaa tcggccggat gacttacacc attgacccag
     3121 gcctttatgc gctggagaat cctgaccggt cttccccggt gctggtcacc gcgaactaca
     3181 agctgacctt tgaccggctg cgccgggctt tgccgggcaa gaatttctgg gtcctggcgc
     3241 tcgacaccaa aggggtcaat gtctggtgcg cggcagggaa ggggaccttt ggaaccgaag
     3301 agctagtgga gcgcatcaag ctgagtggcc tggaaaaggt agtggaccac cgggagttga
     3361 ttcttcccca gctgggggcc cccggggtgg ccgcccatca ggtgagaaag tcatcggggt
     3421 ttaaagtcat ctacggaccg atcctggcca aagacctccc tgcctttctt gaagcgggga
     3481 aaaaggccac cccagagatg aggcgcaagg cttttactgc atgggaaagg gcggtcctca
     3541 tccccattga attggtccac gtcctgaaat ggatggttat tctacttcca gctttattct
     3601 tattgggggg ccttgccgga ggagggactt tctggcagaa cgccctgacc catggggtct
     3661 tggccgcggc tgctctcatc ctctccctga ttatgggcgc agtgctgacc cccttgctgc
     3721 tgccttttct gcccgggcga gcctttgcct tgaagggatt tttcttgagc atcttgggag
     3781 ttatattcct cctggtagta ttcggcggag gggagatgaa ctggccggga atcctggaga
     3841 ggctggcctg ggttcttctg gtgccggcgc tcaccgcttt cctggccatg aacttcactg
     3901 gggcttcgac ttatacttcc ctttcgggag tgaaaaagga gatgcgctgg gctttgcccc
     3961 tggagatcgg gggcgcggcc cttggcctgg ttttttggct gggagcgctc ttttatttat
     4021 gatttttatt gggacgcaga taaacgcaga ttttcaggat ttaagagtgc taaagacctt
     4081 ttattatcaa ttcagcgctt tgtctttctt tacccatttt acgaattcgt cttttctgcg
     4141 tatatctgcg ttcatctgcg tcccggaaga aatggaggag tggggaaatg aatcgcatgg
     4201 tctatttgaa gaatgtcgcc acactgcggg tctttgagga caaatgcgta cgctgcggga
     4261 tgtgcctgga agtttgtccc catgcagtcc taagcctgaa tgggggaaag gtaaaaatcg
     4321 aagaccggga tgcctgcatg gaatgcgggg cctgcgccca gaattgtccc gccggagcga
     4381 ttgtggtcaa atccggggtg ggatgcgcca acgcggtcat caattccatg ctgggacgca
     4441 aaagcgattc ctgctgctgc gtcatcgagg aaaagggtac ggcttcggag gcctgcacgg
     4501 ataacaaagt gtgctgctga tgaccgcgcg ggatgcttag attctgggta tcagatcgcg
     4561 gatatttgac ggtagatata agggacaaca agcatccaga aacgagcaac cagcaacaag
     4621 caacgagtaa cgaactctac gcttccttct tccccttgga tttggttgcc tggattccga
     4681 gcccctcaaa gaggaaaaga atggagaatc catacaggag agtgtagagg agataaaaac
     4741 ccagaaagag ggcgaccgtc cagatatttt ccccaattgg ccgggtttca attcccgcaa
     4801 agttgtaggc cggctcgagt ac
//
LOCUS       DASPRR010000105        10957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1150138, whole genome shotgun sequence.
ACCESSION   DASPRR010000105 DASPRR010000000
VERSION     DASPRR010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150138"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /gene="murF"
                     /locus_tag="VEL68_03745"
     CDS             complement(<1..1376)
                     /gene="murF"
                     /locus_tag="VEL68_03745"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXZ35114.1"
                     /translation="MRFWEKRRSTSTTARRLRKRSLCSGGRPIVNLSAGEIAQATQGT
                     IIHGSSSCHIRGVSSDSRKIQAGELFVPIQGPNFDGHQFIGQALERGAAGSLARPGWE
                     PKAGGNDASGKFLIRVEDPLRALGDLAHFWRGRHSRVKVVAITGSNGKTTTKEMAAQT
                     LSAAFRVLKTEGNLNNLIGLPLMLLRLSDEHEVAVLEMGMNAPGEVRRLKDIAAPQVS
                     LITNVGRAHLEFLGSLQAIARAKGELWEGLNPEDWIAVNADDPRVVELAAAVHCRKKT
                     FGIRKRAEISAEDFYVEVAWGTRFSLRLDGKRIPIRLNTFGRHNVYNALGASALAAIL
                     GLDAEKIAAGLETFHPYPGRGRIHRLGRGLHLLDDTYNSNPDSLQATLAAFGEMKGET
                     RGLVVLGDMLELGPGTAGFHEEAGKFVGALGFEHLFLCGEQREHLAEGAKSAGMEESR
                     IHTFANPE"
     gene            complement(1285..2808)
                     /locus_tag="VEL68_03750"
     CDS             complement(1285..2808)
                     /locus_tag="VEL68_03750"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXZ35115.1"
                     /translation="MKDLLSCLPQKEVRGDTGVEVKGLAYHSAAVQEGFLFAAIRGMK
                     DDGTRYLEDAFARGARVILLDRPLEARGPVQVVVPDVREALARLASAFYGNPSSSLTL
                     IGITGTNGKTTTSYLLESILEEDGKKVGVMGTVNYRFQGRAFAAPTTTPESLDLQKNL
                     QAMREGGVTHAVLEVSSHALDLHRVRGCDFDVALFTNLTRDHLDYHRSMDEYFRAKQL
                     LFTQCLRESKKEKRFAVLNADDPKAEELVRMACGASFRYGLERKGDLWARSYEEGPEG
                     LRFQLVTPRGSLEGESPLIGRHNLYNLLAAVSVAEVLKVSHRAILQGIAGLTRVPGRL
                     ERIPGGDGVRVFVDYAHTPDALERALETLQKGRGGRLIVVFGCGGDRDRGKRPEMGRV
                     ASAGSDLAVITSDNPRTEDPLRIVEEIEQGVNPQRRKKYKAPPLSGGWEERGYMVIPD
                     RRAAIRAAIGAARAGDIVLIAGKGHEDYQILGEEKVHFDDREEATQALALLRGKANR"
     gene            complement(2966..4939)
                     /locus_tag="VEL68_03755"
     CDS             complement(2966..4939)
                     /locus_tag="VEL68_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXZ35116.1"
                     /translation="MKEPPQTWLRFRITLLLYLFSFLFLVIFGRAYQLQVLQSQRLGA
                     MAERQSQRIVQLIPKRGIIYDRKKEEMAISVEVDSVFAQPGKVENVREVAQKVAPLLG
                     KKPSDLLSKLKGEEPFIWLQRGISPEQRKAIERYDFSGVAFLKETKRFYPQGGLGVHV
                     IGFAGLDGQGLEGVEVGYDEFIRGEPGYIVISKDALGRSLTSQTAPSRQSLDGCDVIL
                     TIDKNIQYVVEKELKRAVQACSANGGMAVVMNPKTGEILALAVQPSFDPNHFSTVPPQ
                     VLKNRAVTDTFEPGSTFKVFLLAAALEEQAAAPKEMFFCENGAYTVGGKVIHDTLKYG
                     WLSLADIVKLSSNIGASKVGKKLGKNRLHRHLKNFGFGAKTGVDLPGEVPGFLAPPRT
                     WSEVGLANISFGQGVSTTALQLTAALAAVANGGVLMKPYVVKGVLDSNGDFLQENRPK
                     VVRRVISPETTAMVTKILKTVMEEGGTGKAARLAGYESAGKTGTAQKALANGRGYSDK
                     RTGSFFGFAPADNPQVVIAVIIDEPQGTSYGGVVAAPAFKAIGEQMLPYMGVYPKGVT
                     YLAKVGVGMSAPKDTTPANSPAQSAPVEASEDPGVMPDFSGKSLRQVVLIAQRLGLDL
                     KLVGSGKAISQNPPPGYVLQGQNRGVVKFSPAS"
     gene            complement(4936..5301)
                     /gene="ftsL"
                     /locus_tag="VEL68_03760"
     CDS             complement(4936..5301)
                     /gene="ftsL"
                     /locus_tag="VEL68_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HXZ35117.1"
                     /translation="MAEAISKRISVLAPGREREQKAASAAEDVAREVNRNLIFLVLMV
                     ALVFIGCSLFYVWSHHQILSLGYETSQAAREEQTLIQENKRLRLELASLKAPSRIERM
                     AQQQLGLGIPQKEQLIIVR"
     gene            complement(5317..6219)
                     /gene="rsmH"
                     /locus_tag="VEL68_03765"
     CDS             complement(5317..6219)
                     /gene="rsmH"
                     /locus_tag="VEL68_03765"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXZ35118.1"
                     /translation="MEERGHLPVLLEEVLQYLDCRPDRIYVDGTVGSGGHSRKILERN
                     SPTGKLIGLDWDEEAIERARTNLSLFEGRVALRKGNFKDLKSVLKSLSIPAVDGILLD
                     LGVSTEQLESRERGFSFRWDGPLDMRMSQETEITAQDLVKNLPPSDLEALLREYGEER
                     WARRIARNIVRRRQKAPIRTTGELVEVIEASVPRSRGRIHPATRTFQALRISVNEELN
                     NLRAFLDLAPSLLRPKGRLCIISYHSLEDRIVKNLFRHWTRGGKGEEKPFGLLTPKPV
                     VPAAEEVSRNPRARSAKLRAVEMN"
     gene            complement(6223..6669)
                     /gene="mraZ"
                     /locus_tag="VEL68_03770"
     CDS             complement(6223..6669)
                     /gene="mraZ"
                     /locus_tag="VEL68_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXZ35119.1"
                     /translation="MFRGRFEHTIDPKGRVSIPAKFRELLREKYDDRLIITIGLNDPC
                     LMAFPYEEWVNVEEKVGSMSMVKKEVTAFQRLVISAAMECPIDKLGRVLIPPNLRAHA
                     QLEKDVVFAGVLKKFEIWNKERFFEMIQKTGEHSEEMGETLAALGL"
     gene            complement(6884..7936)
                     /gene="mltG"
                     /locus_tag="VEL68_03775"
     CDS             complement(6884..7936)
                     /gene="mltG"
                     /locus_tag="VEL68_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXZ35120.1"
                     /translation="MAKSIPSPIKPRRSLWEWCKERGLDLFITLGGTVAVLLATIYLH
                     FWAPPSLTKNTKVVVIPQGRSFHEIARVLEDHGIIRDRRSFYLLARIEGSLSKVRAGE
                     YEVHTRMTPSEVLSKLVRGEVIQYPIFIPEGCNIYQIGEILEQNKVCSKKIFLEKVQD
                     PGLIQTLGLEGDSLEGYLFPDTYNFPKGLGEEQVIRQMVSRFKTVWSSLAKRAETMGL
                     SRRDVVILGSMVEKEAKDDQERRFIAAVFHNRLNRGMALQSDPTAIYGLKEKRSSITR
                     GDLQRRTPYNTYIISGLPKGPIANPGFKSLQAVLYPAEVNYLYFVSKNDGTHFFSNTL
                     QEHNLAVAKYQRRGKK"
     gene            8643..9647
                     /locus_tag="VEL68_03780"
     CDS             8643..9647
                     /locus_tag="VEL68_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HXZ35121.1"
                     /translation="MNGSITDVPGFQVGHAQDFKGATGCTVVLCPPNTVGGVDVRGSA
                     AGTRQADSLQPFHFVDEVHAVLLSGGSAFGMDASGGVMRFLEERSIGYPTSAGKVPIV
                     PTAILYDLGIGEGRIRPNQEMGYQACVNSHPGRVEEGSIGVGTGASVGKLMGILWATK
                     GGVGTTSLILPSGIIVAALVVVNAFGDVIDLQEGKIIAGARDPQNPGVFLDSAARIKE
                     SAIPFRAEKGFENTTLGVVATNVKLSKRQAIKVAQMAQSGFSRTIRPVHSTVDGDLIF
                     ALSSGDRNADVNAVGLMAEDAVVVSVHRAIREADGLGYLPAHKDLVRISEKGSSGSKS
                     "
     gene            complement(9659..10633)
                     /locus_tag="VEL68_03785"
     CDS             complement(9659..10633)
                     /locus_tag="VEL68_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXZ35122.1"
                     /translation="MNFEEAIQLVEKEMKAVEADMAKNFLSEIAMIPTVSRYLISSGG
                     KRFRPLLLLLSARLCGHSGPRAIPLASSIEFIHTATLLHDDVVDRAFLRRGLASANSV
                     YGDGASVLVGDFLFTKAFSMIVGEGNLRILEVVSNTTTRMAEGEVLQLTKMGNPETTE
                     EDYRYVVINKTAVLISAACQIGGILGGVPEEKEKSLAAFGLNLGIAFQLMDDTLDYIS
                     EEKTLGKTIGKDLSEGKVTLPLIHTLRAPPAGSQERLRAVIRNQDRTEEDLRLVMQAV
                     RDSGGIDYTVMRATGYVKEAQKFLSTFSPTPEREALLAISEYTLKRKK"
BASE COUNT         2247 a         3256 c         2982 g         2472 t
ORIGIN      
        1 tcttccgggt tggcaaaggt gtgaatccgg ctctcctcca ttcccgcgga tttcgccccc
       61 tcagccagat gttcccgctg ttccccgcaa aggaagagat gttcgaaccc cagggccccc
      121 acaaactttc ccgcttcctc atggaaaccc gcagtccccg gtccaagctc taacatgtcc
      181 cccaaaacca ccaatccccg ggtttccccc ttcatttctc cgaaggccgc cagggtggcc
      241 tgcagggaat ccgggttgga gttgtaggtg tcatccagaa gatgcagacc ccggcccagg
      301 cggtggattc tccctcgtcc gggatagggg tggaacgttt ccagcccggc cgcgatcttc
      361 tcggcgtcta atcccagaat ggcggccaga gccgaagccc ccagagcgtt gtatacattg
      421 tgcctcccga aggtattgag ccggatgggt attctcttac catctagcct cagggaaaac
      481 cgggtccccc aggcgacctc cacgtagaaa tcttcggccg aaatctccgc ccgcttccgg
      541 attccgaagg tcttcttgcg gcaatgaacc gcggcggcca attcaaccac ccgcgggtca
      601 tcggcgttga ccgcaatcca atcttcggga tttaatcctt cccagagttc acccttggcc
      661 cgggcaatcg cttgcaggct ccccaaaaac tccaggtggg cccggcccac gttggtaatt
      721 aatgagacct ggggggcagc gatatctttt agcctccgaa cctcccccgg agcgttcatc
      781 cccatctcca ataccgccac ttcgtgctca tcggatagcc gcagaagcat cagcggaagc
      841 ccgatcaggt tattcaaatt cccttcggtc tttagaaccc gaaaggccgc cgacaaggtc
      901 tgggcggcca tctctttggt cgtggtcttc ccgttgctcc cggtgatcgc caccaccttt
      961 acccgggaat gccttcccct ccagaaatga gccaaatccc ccaaagctct gagagggtcc
     1021 tccacccgga tgagaaactt tccggaagca tcgttcccgc cggctttggg ctcccagcca
     1081 ggccgcgcga gacttcccgc agcccctctt tccagggcct ggccaataaa ctgatgtccg
     1141 tcaaagttcg gcccctggat gggaacgaac agctctccgg cttgaatctt gcgcgaatca
     1201 ctgctgactc cgcgaatatg gcaagaagag cttccgtgaa ttatcgtccc ctgggtggcc
     1261 tgggcgattt ctcctgccga gagattaacg attggccttc cccctgagca aagcgagcgc
     1321 ttgcgtagcc tcctcgcggt cgtcgaagtg gaccttctct tctcccagaa tctgatagtc
     1381 ttcgtgccct ttgccggcga tgaggacgat atcccccgcc cgggctgcgc cgatggccgc
     1441 tcgaatggcc gctcttcggt ccgggatcac catatatccc cgctcttccc acccgccgga
     1501 cagagggggg gctttatact tttttcgtct ttgagggttg accccttgtt cgatctcttc
     1561 gacgatcctc aaggggtctt cggtgcgggg attatccgaa gtaatcaccg ccagatcact
     1621 ccctgccgaa gccacccttc ccatctccgg gcgttttccc cgatcccggt cgcccccgca
     1681 tccgaaaacc acgatgagtc ttcccccacg gcctttttgc agagtttcca gggctcgctc
     1741 cagcgcatct ggcgtatgcg catagtctac gaacacccgc actccgtcgc cccccggaat
     1801 ccgctcgagc cttccaggga cccgggtgag cccggctatt ccctgaagaa tggctcgatg
     1861 ggacaccttc agcacttcgg ccacgctgac cgccgccagg aggttgtaga ggttgtgccg
     1921 gccgatcaat ggggactccc cttccaggga accccggggc gtaaccaact gaaacctcaa
     1981 cccttccgga ccctcttcat aacttcgggc ccaaaggtcc ccctttctct ccaggccgta
     2041 acggaaggac gcgccgcagg ccattcgcac cagttcttca gccttcggat cgtccgcgtt
     2101 gaggaccgca aaacgcttct cctttttgct ctctcgaagg cattgggtga agaggagctg
     2161 cttggcccga aaatattcat ccatggaccg atgataatcc aaatggtccc gggtcagatt
     2221 cgtaaagagg gccacatcga aatcgcatcc ccggacccga tgaaggtcca gagcatggga
     2281 ggaaacctcc aggaccgcat gggtgacccc gccctcccgc atggcctgaa ggttcttctg
     2341 caaatccagg gattcggggg ttgtggtggg agcggcaaag gcccttccct ggaagcggta
     2401 attgaccgtt cccatcaccc ccactttctt tccgtcttct tctaggatag attcgaggag
     2461 gtaggaggtc gtggtcttgc cgttcgtgcc ggtgattccg atcagagtga gagaggagga
     2521 tgggttccca tagaaagccg aagccagtcg ggccagagcc tcccgcacgt cgggaaccac
     2581 aacctgcaca gggccccgag cctctagagg tcgatccaga agaataaccc gagcccctcg
     2641 ggcgaaggcg tcctccaggt acctcgtccc atcatccttc atcccccgga tggctgcgaa
     2701 gagaaagcct tcctgaaccg ccgccgaatg gtaggccagt cctttgactt cgaccccagt
     2761 atcgccccgg acttccttct ggggcaggca ggaaaggagg tctttcaaaa acaatttttc
     2821 tttcgatcct tgcgaagaat taattaatta tactcctttt ccccggtggg gcaaaaaaac
     2881 acagttccaa gttgccggtt gcaggtccca ggtcaaaaac caaagaaaga gactcgtgac
     2941 tggttattcc tgccccctgg gactgtcaac tcgccggcga aaatttcact accccccggt
     3001 tctggccctg aagcacgtag cccggagggg ggttctggga aatggctttc ccgctcccca
     3061 caagcttcag gtcaaggccc agccgctggg cgatcaaaac cacctgcctc agggattttc
     3121 ctgaaaaatc gggcataacc ccggggtcct cggaagcctc gaccggggcc gattgagccg
     3181 gcgaattggc aggggtcgta tctttggggg cggacatgcc tacccccact ttggctaggt
     3241 aggttacccc tttgggatag actcccatgt aagggagcat ctgctcgcca atggctttga
     3301 aagctggcgc agcgaccacc cctccataac tggtaccctg gggctcgtcg ataataacgg
     3361 ctatgactac ctgggggttg tccgcggggg caaacccgaa gaaagaaccg gttcgcttgt
     3421 ctgagtatcc ccggccgttg gctagggcct tctgggccgt ccccgttttg cccgccgatt
     3481 cgtagccggc gaggcgggcc gctttgccgg ttcccccttc ctccattacc gtcttcagaa
     3541 ttttcgtcac catggccgtc gtctcgggag aaatcacccg ccgaacgact ttgggacggt
     3601 tctcctgcaa gaagtcccca ttcgaatcaa ggactccctt gaccacgtag ggtttcatta
     3661 acactccccc gttggctacc gcagccagag cggcggtgag ctggagggcg gtagtggaaa
     3721 cgccctgtcc gaaactgata ttcgccagcc ccacctccga ccaggtgcgg ggtggggcaa
     3781 ggaagccagg gacctccccg gggaggtcca ccccggtctt ggccccgaac ccgaaattct
     3841 tcaggtgacg gtgcagccta ttctttccca gcttcttccc caccttgctt gctccaatgt
     3901 tactggagag cttgacgatg tccgccagag aaagccaccc atacttaagg gtgtcatgga
     3961 tgacttttcc tcccaccgta taggccccat tttcgcagaa gaacatttcc ttgggggcag
     4021 cggcctgctc ttccagggct gcggccagga ggaagacctt gaaagtcgag ccgggctcga
     4081 aggtatcggt aaccgcacgg ttcttgagga cttggggagg aacggtggaa aaatggttgg
     4141 ggtcgaagga aggctgaacc gccagggcca ggatttctcc ggtcttaggg ttcatcacca
     4201 cggccatccc cccattggcc gagcaggctt gaaccgccct cttcaattct ttttccacga
     4261 catactggat gttcttgtcg atcgttagga tgacatcgca gccgtccagg gactggcggg
     4321 aaggagcggt ctgggaggtg agggaccgcc ccaaggcgtc cttggagatg acgatgtatc
     4381 ccggttctcc acggatgaac tcatcgtagc cgacctcgac cccttccaaa ccctggccat
     4441 ccaggccggc aaagccgatg acgtgaaccc ccagtcctcc ctgaggatag aaccgttttg
     4501 tttccttcag aaaagcgacc cccgagaaat cgtatcgctc gatggctttt cgctgttccg
     4561 gagagattcc cctttgcagc cagatgaaag gctcctcccc tttcagtttg ctgaggagat
     4621 cggatggctt cttgcccagc aggggagcga ctttctgggc cacctcccgg acgttttcca
     4681 ccttgcccgg ctgggcaaag accgagtcaa cttccacgct aatggccatc tcctccttct
     4741 tccggtcgta aataatgccc cgtttgggaa tgagctggac gatccgctgg gactgccgct
     4801 cggccatggc cccaaggcgt tggctctgga ggacctggag ctgataggct cgcccgaaga
     4861 tgaccagaaa aagaaaagag aagaggtaaa ggaggagggt gatacggaag cggagccatg
     4921 tctgaggggg ctctttcatc gtacgatgat caactgttcc ttttgcggga ttcccaaacc
     4981 cagttgctgc tgggccattc tttcgatgcg gctaggggcc ttgagcgaag ccagttccag
     5041 acgaagccgc ttgttctcct ggatcagggt ctgttcttcc cgggcggcct gggatgtttc
     5101 atagcccagg gagaggattt gatgatggga ccagacgtag aaaagagagc atccaatgaa
     5161 caccaaggcc accattagga ccaggaagat caggttgcgg ttaacctccc gggcaacgtc
     5221 ttcggccgcg gaagccgcct tttgttcccg ttccctcccg ggggccagga cggagattct
     5281 tttggaaatt gcttcagcca cgattcgctc ctcggttcaa ttcatttcca cggctctcag
     5341 cttggcgctg cgggcgcggg gatttcggga aacctcttcc gccgcgggga ctacaggctt
     5401 gggagtgaga aggccgaagg gtttttcttc tcccttcccg ccccgggtcc aatggcggaa
     5461 aagattcttt acgatccggt cctccaggga gtgataagat ataatgcaca gccggccctt
     5521 ggggcggagc agggacggcg ccaagtcgag gaaagccctg aggttattca gttcttcatt
     5581 caccgatata cgcagggcct ggaaggttct cgtggccggg tgaattcgcc cccttgatct
     5641 gggaacggat gcttcgatca cctccaccag ttcccccgta gtgcgaatcg gagccttctg
     5701 ccgtcggcga acgatattcc gggcgattct tcgggcccac cgctcttccc cgtactcgcg
     5761 gaggagcgcc tccaggtccg aaggaggaag attctttacc aaatcttggg cggtgatctc
     5821 tgtctcctgg ctcatgcgca tgtccaatgg accatcccaa cggaagctga atcctctctc
     5881 ccggctctcc agctgctcgg tagaaacccc aaggtccagc aggattccgt ctacggccgg
     5941 gatggagaga gacttcaaaa cggattttag gtctttgaaa ttcccctttc tcagcgcgac
     6001 tctgccctcg aagagcgaca ggttcgttcg ggcccgttca atggcctcct catcccagtc
     6061 cagcccgatc aactttccag tgggagagtt tctctccaga attttccggc tgtgccctcc
     6121 gctgcccacg gttccatcca cgtaaatccg gtccggcctg cagtccagat attggagaac
     6181 ttcctcgagc agaaccggca gatggccgcg ctcctccatg gattagaggc ccagggcggc
     6241 aagggtctcg cccatctcct cggaatgctc tcccgtcttt tgaatcattt cgaaaaaccg
     6301 ttccttgttc caaatttcga acttcttgag caccccggca aagaccacgt ccttttccaa
     6361 ctgggcatgg gcccgcagat tgggagggat caggacgcgg ccgagtttgt cgatgggaca
     6421 ttccatagct gcggagataa ccaacctctg aaaggctgtg acttccttct ttaccatgga
     6481 catggagccg accttctctt ccacgttaac ccactcttcg taggggaacg ccataagaca
     6541 ggggtcgttc aggccgatgg tgatgatcaa ccgatcgtca tatttctccc tgaggagttc
     6601 ccggaatttg gccgggatgg atacccttcc cttagggtct atggtatgct caaacctgcc
     6661 cctgaacata tctcagttac cttaatgcgc caaaatatcc ctctatatac ctattttttc
     6721 ccttttatac caccttatgg tcaatatacg ggcttaccag aatgatgtca agaaaattct
     6781 atctatttta ttaatttttt agatgagatg ggggggctgg agatggtcca ttggtaatcc
     6841 gaacaaaatt gtacaacaaa tttcttgact cctctgccga gtcttatttt ttccctctcc
     6901 tctgatactt ggccaccgcc aggttgtgtt cctgaagggt gttggaaaaa aaatgggtgc
     6961 catcattctt ggagacgaaa tagaggtaat tgacttcagc ggggtacaat accgcttgga
     7021 gggatttgaa acccgggttg gcgatcgggc ctttgggaag gccgctgatg atgtaggtgt
     7081 tgtaaggagt ccttcgctgt aaatcccccc gagtaatgga acttcgtttt tccttcaatc
     7141 catagatggc cgtaggatcg ctctgcaaag ccattccccg gttcaggcgg ttatggaaaa
     7201 cggcggcgat gaatcttctc tcctgatcat ctttagcctc tttctcaacc atagaaccta
     7261 ggataaccac gtctctgcgg ctcaggccca ttgtctcagc tcttttggcc agggaactcc
     7321 aaaccgtttt gaaacgggac accatctggc gaatcacctg ctcttccccc agccctttgg
     7381 gaaagttgta ggtatccggg aaaagatatc cctccaggct gtctccttcc aaccccaggg
     7441 tttggatcaa gcccgggtct tgcacttttt ccaggaatat cttcttggaa cataccttgt
     7501 tctgttccag aatttctcca atctggtaga tgttgcagcc ttccggaatg aagatggggt
     7561 attgaatgac ctcccctcgg actagtttag agagaacctc gctgggggtc attcgggtgt
     7621 ggacttcata ctctcccgct ctcactttgg aaagagaacc ttcgatccgg gccaaaaggt
     7681 aaaagctcct ccggtcccgg atgattccat gatcttccaa aacccgggcg atctcatgaa
     7741 agctccttcc ttgagggatc accaccactt ttgtattttt ggtcaaactg gggggagccc
     7801 aaaaatgaag atagatggta gccagcaata ccgccaccgt tcctcccaag gtgatgaaaa
     7861 ggtccaaccc cctctctttg caccactccc agagggaacg ccgcggtttg atggggctgg
     7921 gaatcgattt ggccattttc ctaaacccag atgcagagct gcaacctgaa acccaacaat
     7981 tgggccatct ttctcaaaga acgattggcc ccacccaata acttcggccc ctaaaaaagc
     8041 aaactgaaac cgaacttcat tagaatttta ggacccaaag cctgggtcgt caagattttt
     8101 cgccatctgt tacgctcccg gtttcattct tcgacctttg gggcggaacc ttgcccctgg
     8161 gccgggcgag gtctccaagt tgccgacgat tacctgattt ctggtgggta ggtaaagggt
     8221 tctcctcttg ccctccttca cttccctttt gtgtttatca tttctgcgaa aaggctggct
     8281 tgtggcccgg aagggaaggc gttgcgcgga aaaagcatcg gcgggaaaag atttgcaacc
     8341 ggtggtccga tcgtgtttcc gcagaatgag gcgggcccat cgggcgcccg gccgagcgct
     8401 cctacaacgg aataaccctg ttcttgggga aagaacggct tgcccgcccg gtccaagaaa
     8461 tcgtccaggc cttatggttt tttcgcgcaa taccctccct tccgggccgg gcgatgcttg
     8521 aattcaatag gcctcacgcc tacttgccca aaagtgaaag ggggggcaaa ggcttctccc
     8581 ccccttgact tccttctccc atggggttag gaataattac tctggctaga agggaaagaa
     8641 tcatgaacgg ctcgataacc gatgttcccg gattccaagt tggccatgcc caagatttca
     8701 aaggagccac tggctgcacg gtggtgctat gccctccgaa tactgtggga ggagtggatg
     8761 ttcgggggtc ggccgccggc acccggcagg ctgattctct ccagcccttt cattttgtgg
     8821 atgaagtcca tgcggtcctc ctctccggag gcagcgcctt cgggatggat gcctccggag
     8881 gggtgatgag atttcttgaa gaaaggtcca tcggctaccc gacgtccgcg ggaaaggtcc
     8941 ctatcgttcc tacagccatc ctctatgatt tgggaatcgg ggaaggccga atccggccca
     9001 atcaagagat gggctaccaa gcctgtgtaa acagccatcc cggaagagtg gaggaaggaa
     9061 gtatcggggt cggcaccgga gccagcgtgg gtaaacttat gggcatcctc tgggctacca
     9121 aggggggggt gggaacgact tctctcattt tgcccagcgg gatcatcgtg gctgccctgg
     9181 tggtggtcaa cgccttcggc gatgtgatcg acctccaaga agggaagatc atcgccggag
     9241 cccgggaccc gcagaatccc ggggtctttc tcgactccgc ggcccggata aaagagagcg
     9301 ccattccgtt tcgggcggag aagggctttg agaataccac tctcggcgtg gtggccacta
     9361 acgtaaaact ctccaaacgg caggctatca aagtggccca aatggctcag agcggatttt
     9421 cccggaccat tcggccggtc cactccacag tcgacgggga cctaattttt gccctttcct
     9481 caggagatcg aaacgcggat gtcaacgccg tcggtctgat ggccgaggat gccgtggttg
     9541 tctccgttca ccgagcgatc cgggaggcgg atgggttggg gtacttgccg gcgcataagg
     9601 atttggttcg gatttcggag aaaggcagtt cggggtcaaa gtcctaactc cctttccact
     9661 actttttcct tttcagggta tattctgaaa tggccaggag cgcttccctc tccggggtgg
     9721 gagaaaaagt ggataggaat ttttgcgctt ctttcacata accggtcgcg cgcattaccg
     9781 tatagtcgat tcccccggaa tcccgcacgg cctgcataac caagcgcagg tcctcctcgg
     9841 tgcggtcctg attccggatg accgcccgca gccgttcctg gctgcccgca gggggggccc
     9901 ggagggtgtg aatcaggggc agggtgactt ttccttcgct caagtccttg ccgatggtct
     9961 tgcccagggt tttttcttcc gagatgtagt ccagggtgtc gtccatgagc tgaaaggcga
    10021 tgcccaagtt caggccgaaa gcggccaagg atttttcttt ttcttccggg acccctccga
    10081 ggatcccccc gatctggcaa gccgcggaga tcaataccgc ggttttgttg atcaccacat
    10141 agcggtaatc ttcctcggtc gtctccgggt tgcccatctt ggttaactgc agcacttccc
    10201 cctctgccat gcgggtcgtg gtattggata ccacctccaa aatccgcagg ttcccttctc
    10261 cgacaatcat ggaaaaggct ttggtgaaga ggaagtcccc caccagcacg ctggctccat
    10321 ctccatatac ggaattggcc gaagccaggc cccggcgcag gaatgcccgg tccaccacat
    10381 catcgtggag gagggtcgcc gtgtggatga attcgatgga actcgcgagg ggaatggccc
    10441 ggggcccaga gtggccacaa aggcgggcgc tcaggagcag caagaggggc cgaaaccgtt
    10501 tgcccccgct ggatatgaga taccggctca ccgtggggat catggcgatc tcggagagga
    10561 agttcttggc catatcggcc tcgacggcct tcatctcttt ctcgaccagc tggatggctt
    10621 cctcgaaatt caagaacctt ctccgcgaaa ggattctgcc tccgggcccg aaaagaagaa
    10681 caacccggcc tgtgagggtc tctgtccccc ctgcccggaa tttctttttc cttctcgccc
    10741 gaggccggga atcagtgggc gaagatttcc atccccgagt catgacctcc accagaggtg
    10801 aaggcatgaa atgcccctga accgctcaaa gcggattgaa aggagcgcca cctgaaggtg
    10861 gccatcacct ggacaccaat gccaggcccg ggggcaaggg tctggggttc aaaaaccatg
    10921 aggcgtcctt catttcatgg gccgggtcgg ccagccg
//
LOCUS       DASPRR010000106         8462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_341265, whole genome shotgun sequence.
ACCESSION   DASPRR010000106 DASPRR010000000
VERSION     DASPRR010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341265"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VEL68_03790"
     CDS             <1..547
                     /locus_tag="VEL68_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35123.1"
                     /translation="IDLLCPKETILASNTSGLSITEIASVTQRPRKVIGMHFFNPVPA
                     MKLVEIIRGQSTSDETFQVIKTLALKMGKTPIAVNESPGFAVNRILIPMINEAIFVLQ
                     EGVASAEDIDKGMMLGANHPIGPLALADLVGLDTLLMIQENLYKELGDSKYRPCPLLR
                     KMVRAGYLGRKNGQGFYAYPK"
     gene            725..1531
                     /locus_tag="VEL68_03795"
     CDS             725..1531
                     /locus_tag="VEL68_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ35124.1"
                     /translation="MVVDNRSKSLIIDQKEGVATITLNRPERLNAIDEAMHRRLEQLF
                     IEMDDDSRVRAIVITGAGRAFCSGGDVKDMDEQQRGPTQRKELAPTRHSRHLLRNMLE
                     CETPIIAAINGDAVGLGASIALLCDVIFAAETARIGDTHVRVGLAAGDGSAVIWSMLC
                     GIARAKQYLMTGDLIPAREAERIGLVNTVVPEGKAYEEAWKFAKRLADGPIQAIKWTK
                     YSVNKLIKDQMNLTLDTSLALESLTFLTEDHSEASRAFVEKRAPLFKHEK"
     gene            1645..3189
                     /locus_tag="VEL68_03800"
     CDS             1645..3189
                     /locus_tag="VEL68_03800"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXZ35125.1"
                     /translation="MSRVRDEIEKFKQIEETARAGGGKKYVEEQHRLGKLTARERIDK
                     FMDPGTFVEVTMLAQHQCTDFGMEKNHPWGDGVITGYGKVEGRTVFLYAQDSTVMGGS
                     VGLVHGQKIASIIRMGRQAQAPVVGLIDSAGGRIQEGSGAYSLIFAENVEASGVVPQV
                     SVIMGNCAGGGVYSPALTDFIFMVDKTSQMFITGPLVIKEVTGQDVGSQELGGARVHY
                     QVSGVADFVGENDLACLAKVKKLLSFFPSSFNQKPSAVPCLDDPDRCEEALVDIIPDS
                     PKKVYDMRRLISLIVDHGDFLEVRAGFARNMVTGLARMNGKVVGIVANNPMFLASAID
                     CDAADKAARFIRTCDCFNIPLISLVDVPGYLPGMKEEHKGIIRHGAKMLYSWKEATVP
                     KITCIIRKAYGGAFAAMSNKELGADLVMGWPSTEIAIMGAEGAVRILFRKEIDAAADK
                     ARVREEKIQEYREKFMTPYYTAAKRQMDVLIRPQETRPQIIKALEMLEGKKVDRAERK
                     HGNIPL"
     gene            3317..4783
                     /locus_tag="VEL68_03805"
     CDS             3317..4783
                     /locus_tag="VEL68_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXZ35126.1"
                     /translation="MKVSVPEKWDMEYDVVVVGWGSAGTAAAVTAHDQGAKVVILEKM
                     ANGGGNSSICGGNIIVPKGKEFLEYLDTLSFKTTEREILETFVEEALKNGDWIRQMGA
                     DVQVFRPLEVAYPTMIAGAGFPHVRGAENVVKYNIKGTPEEGKPSQRLWKFLSGLVSK
                     RGIKVLTATPAQELVPNARGEIVGVIAQREGKRISIKARRGVILTCGGYENDPWMKWD
                     YLPVKPLYFLGTPGNTGDGIRMVQKVGGELWHMTRLACLIGFKAPEFEAAFWVAFLSE
                     GFLFVDKYGRRFVNEAGIEIHEYYKELSHFDIEKMEYPRIPLWAIFDEQTRRRGPLSR
                     GTAGYNRDLYSWSLDNSVEIAKGWVQQGKTVAELASKISMEPKVLEETLARFNYLCKA
                     GKDEDFGRPREDLRALESPFYAIQLWPSLINTQGGPRRDKESRVLDPDRKPVPRLYAA
                     GELGSIWGFLYQGACNIGEALVFGRIAGKNAAAEKPWS"
     gene            4792..5241
                     /locus_tag="VEL68_03810"
     CDS             4792..5241
                     /locus_tag="VEL68_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXZ35127.1"
                     /translation="MAGTALAEREFIVNATPDRVWRLIGKVIFSVLPGMESMEILDEN
                     NFRSLLRVKMMGSEWVLKLKGEMVDISPPESFSVRLLLEGLGGLVKADQKVTLAMTPV
                     EKGKTSVTCRATAEDMGFLQRAFLTRQARVFAQSTFEAIEKRLKELA"
     gene            5418..6779
                     /locus_tag="VEL68_03815"
     CDS             5418..6779
                     /locus_tag="VEL68_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ35128.1"
                     /translation="MMGATQILADYVSETSFPALPKEVVRKAKGIVLDTLGCAIAGYT
                     LAAHEFRWVYEPVKEMGGNPESTVWLAGMKTSCPQAALVNGTLVHTVDFDDTHLGSIA
                     HLGASVVSSALAIGEKVGADGPSILTAMVMAYEAAGRIGKAIMPSHYKYWHPTGTLGT
                     IASAVVASKLLGLKADRMEQAISLAADGASGMRYCIDFGDFSKSLHPGFSAWKGILSA
                     LVIASGAAGPKGLLEYKSGFCEAYSDEPAMKHLNESLGSSYEILTDSLKAFPTILCSH
                     TPIQGTVKLMKEKNLRLEDIEAIHFRVTPTAPGQGMNYAPDTPLAARLSIPYCVSRAA
                     ADGYIAMDQFQEEKIKDARVQQFMKKVTLESVQQFVEKYPGTLAAQVEIQTKDGMRFQ
                     DESIYPKGHPQNPMTDEEIKDKFRRLSLNTLDRVQTEKIAEKVYDLEWVKRADELIRL
                     LVK"
     gene            6938..7339
                     /gene="mce"
                     /locus_tag="VEL68_03820"
     CDS             6938..7339
                     /gene="mce"
                     /locus_tag="VEL68_03820"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXZ35129.1"
                     /translation="MIRKIAHIGVAVKNVEEVEKLYSEILGLPISGREVSPDNKVSFI
                     PLGDTAIELLESVKPDGAIARHIEKRGEGIHHIALEVHDIEKAMEELKAKGLVLIDQK
                     PRRGAHDSKIAFLHPKGTYGVLLELVEPKKH"
     gene            7528..>8462
                     /gene="dctP"
                     /locus_tag="VEL68_03825"
     CDS             7528..>8462
                     /gene="dctP"
                     /locus_tag="VEL68_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ35130.1"
                     /translation="MRRIWKGFAVAALAFALAVWMSPGPVSAAEKVFEFKISIDTVPN
                     HPRYGGLEIFVPELAKRSGGRLVGKIYHSAQLYKDAHVTKALRTGTVEMCVPGNWLLE
                     GFDTSASLTMLPMFFGQPGDITEKLIDGKIGEAVNRSLEKKVGAKSPGRWYELGYDHT
                     WTRNKKITKIEDFKGLKIRHSGGSIPEERLNRFGASAVFIAWPDVPMALVQGTVDGVC
                     STTKSVESAKLYEAGIKYAVTCRNYNGYYVPLVNQKFWNSLPADLQKTFSDVWNEIVP
                     KQREIARKEQAEARKLMEGKGVEFFDPSDEELAKWR"
BASE COUNT         2052 a         2246 c         2501 g         1663 t
ORIGIN      
        1 catcgacctc ctttgcccca aagagacaat cttggcctcc aatacttcgg gcctttccat
       61 taccgagatc gcctcggtca cgcaacgccc ccggaaggtg atcggcatgc attttttcaa
      121 ccctgtaccg gccatgaagc tcgtggagat catccgaggg caatccactt ccgacgagac
      181 ttttcaggtc attaaaaccc tggcgttaaa aatgggcaag actccgatcg cggttaacga
      241 atccccggga tttgctgtca accgcatcct catacccatg atcaacgagg ccattttcgt
      301 cctgcaagaa ggagtcgctt ccgccgagga catcgataag gggatgatgc taggagcgaa
      361 ccatccgatc gggcccttgg ccctggcgga tttggtgggc ctggacaccc ttcttatgat
      421 ccaggagaat ctttacaagg agttggggga ttccaagtac cgtccgtgcc cgcttttgcg
      481 gaaaatggtt cgagccgggt acttgggccg aaaaaatggc caagggttct atgcgtatcc
      541 aaagtgaatc aggagtcgct taggattcgt cccaggcgac ctttttagaa tccaacggaa
      601 ttggtccctg aggaaatctt tttcacccga ccattcgtac ccagaatatc cgtccaaaga
      661 ggcctgaaaa tataggccct ttctcgactt tgcgaatact caaaaaatac ccggagggat
      721 agagatggtg gtagacaatc gttctaagtc tttaattatt gatcaaaaag aaggggtggc
      781 aacgatcacc ctgaaccgcc cggaacgact caacgccatt gacgaggcaa tgcaccggag
      841 actggagcag ctctttatcg agatggacga cgattccagg gtccgggcca tcgtcattac
      901 cggggccggc cgggctttct gctcgggggg cgatgttaag gacatggatg aacagcagcg
      961 gggtccgact cagagaaaag aattggctcc cactcgccac agccgccatt tgcttcgcaa
     1021 tatgctggaa tgtgaaaccc ccatcatcgc cgccataaac ggcgacgccg taggactggg
     1081 ggccagcatc gccttgctct gtgatgtaat cttcgctgcc gagacggccc gcatcgggga
     1141 tacccacgta agagtgggcc tggccgctgg ggatggaagc gcggtgatct ggagcatgtt
     1201 gtgcggaatt gccagagcca agcaatacct gatgaccggc gacctgatac ccgcccgtga
     1261 ggcggaacga attgggctgg tcaatacggt agtccctgaa gggaaggcct atgaggaagc
     1321 ttggaagttt gccaagcgcc tggccgatgg acccatccag gccataaaat ggaccaagta
     1381 ttcggtcaac aagttgatca aggatcaaat gaatctaacc ttggatacgt cattggcttt
     1441 agaaagtctc accttcctca ccgaggatca ctccgaggcg tcaagggcgt tcgtagagaa
     1501 gcgggccccg cttttcaagc atgaaaaata attcggtgta aatttcagtt gacaaaaagg
     1561 ggagacttat ctatttgtcc ataaatccgc atatgttatg atctaaacga tagccggcca
     1621 aaggccggcc gggaggagca tgatatgtct cgcgtcaggg acgaaatcga aaaattcaaa
     1681 cagattgaag aaaccgcacg ggcgggggga ggaaagaagt atgtcgaaga acagcaccgc
     1741 ctagggaagc tcaccgcccg ggagcggatc gataaattta tggaccccgg taccttcgtc
     1801 gaggtgacca tgctggccca gcaccagtgc accgatttcg ggatggagaa gaaccacccc
     1861 tggggtgacg gggtgatcac cgggtatggg aaggtcgaag gccggacggt gttcctgtac
     1921 gctcaagact ccacggtcat ggggggatcg gtgggcctgg tccacgggca gaaaatcgcc
     1981 tccatcatcc gcatgggccg gcaagcgcag gcgccggtcg tgggcctcat cgactccgca
     2041 gggggacgaa tccaggaagg aagcggggcg tactccctga tcttcgcgga aaacgtggaa
     2101 gcttccggag ttgttcctca ggtctcggtc atcatgggaa actgcgcggg cggcggggtt
     2161 tactccccgg cgctcaccga cttcattttc atggtggata aaaccagcca gatgttcatc
     2221 accggcccct tggtgatcaa ggaagtcacc gggcaggacg tgggaagcca ggaactgggc
     2281 ggggcaaggg tccactatca ggtctccgga gttgcggatt ttgtcggcga gaatgacctg
     2341 gcctgcctgg ctaaagtaaa gaagcttctg agcttctttc cctccagttt caatcagaag
     2401 ccatcggccg ttccgtgcct ggatgatccg gatcgttgtg aagaggctct ggtggatatc
     2461 atccccgaca gccccaagaa agtgtacgac atgcgccgtc tgatctcctt aatcgtcgat
     2521 cacggggatt ttctggaggt cagggccggc tttgcccgga atatggtcac tgggctggcc
     2581 cgcatgaacg ggaaggtcgt gggaatcgtt gccaataatc ccatgtttct ggcctcggcc
     2641 atcgactgcg atgccgcgga caaggcggcc cggttcatcc ggacctgcga ctgcttcaac
     2701 atccccctca tctctctggt ggatgttccc gggtatctcc ccggaatgaa ggaagagcac
     2761 aaggggatca tccggcacgg ggccaaaatg ctctactcct ggaaagaggc caccgtgccg
     2821 aaaatcacct gcatcatccg gaaagcctac gggggtgctt tcgccgccat gagcaacaag
     2881 gagctggggg cggatttggt tatgggctgg ccctcaacgg agatcgctat catgggtgcc
     2941 gagggggcgg tgcggattct cttccggaaa gagattgatg ccgccgcgga caaggcccgg
     3001 gtccgcgaag aaaagataca ggaataccgg gagaagttca tgactcccta ctacaccgcg
     3061 gcgaaacgtc agatggacgt attgatccgc ccgcaggaga cccggcccca gatcatcaaa
     3121 gccctggaga tgctggaagg aaagaaggta gaccgggcgg aacgaaagca tgggaatata
     3181 cccttgtaag gaaaagtcga aataaaaagc gcagaggccg caacgaaaat caccccctcc
     3241 ctaccctccc cccgcgaggg ggagggttag ggtggggggg atgcgcgttt tcactgttaa
     3301 gggaaaggag aaggaaatga aggtaagcgt gcctgaaaaa tgggatatgg aatacgatgt
     3361 ggtggtcgtc ggctggggct cagcgggaac ggcagcagcc gtcaccgccc atgaccaagg
     3421 cgccaaagtg gtcatcctgg agaagatggc caacggggga gggaattctt ccatctgcgg
     3481 cggcaatatt atcgtcccca agggtaagga attcctggag tacctggata ccctttcctt
     3541 taaaaccacg gaaagggaaa tcctggagac cttcgtggag gaagcgctca agaacgggga
     3601 ttggatccgg cagatggggg ccgacgtcca ggttttccgc ccgctggaag tggcctaccc
     3661 caccatgatt gccggagcag gcttccccca cgttcgcggc gcggaaaacg tggtgaaata
     3721 taacatcaag ggaactccgg aagaagggaa gccgtcccag cggctgtgga aatttctttc
     3781 cggcctggtg tccaaacggg gaatcaaagt tctgaccgcg actcccgccc aggaactcgt
     3841 tcccaatgcc cggggagaga tcgtgggagt gattgctcaa agggagggca agagaatctc
     3901 catcaaggcc cggcggggcg tcatcttaac ctgcggcgga tacgagaatg acccttggat
     3961 gaaatgggat tacctgccgg tcaaaccgct ctatttcctg ggaacccccg ggaataccgg
     4021 cgatgggatc cgcatggtgc agaaggtcgg cggagaactg tggcatatga cccgtctagc
     4081 ctgtctgatc ggcttcaagg ctcccgagtt tgaggcagcc ttctgggttg ccttcctaag
     4141 cgagggattc ctcttcgtgg acaagtacgg ccggaggttt gtcaacgagg cggggatcga
     4201 gattcacgag tactacaagg agctctccca ttttgacatt gagaagatgg aatatccccg
     4261 gattcctctt tgggcgatct tcgatgagca gacccggcgt cgggggcctc tcagtcgggg
     4321 aaccgcgggc tataaccgag acctctactc ctggagcctg gacaactcgg tggaaattgc
     4381 caaaggatgg gtccaacagg ggaaaacagt agccgagttg gcttccaaaa tatccatgga
     4441 accgaaagtt ttggaggaga cccttgcccg cttcaactat ctctgcaaag cggggaagga
     4501 cgaagatttc ggccggccca gggaagacct tcgtgctctg gagtctccct tctacgccat
     4561 tcaactctgg ccttcgctga tcaataccca gggaggcccg cggagggaca aggaatcccg
     4621 cgtcctcgac cccgacagga agcccgttcc cagactctac gccgccggag agctcggttc
     4681 gatctgggga ttcctctacc agggggcctg caacatcgga gaggccctgg tcttcgggcg
     4741 cattgcggga aagaacgcgg ccgcagagaa accctggtcg tgagaattcc catggcaggc
     4801 acagctctgg cggagagaga atttatcgtg aatgcaaccc cggatcgggt ctggcggttg
     4861 atcggcaagg tcatcttcag cgtccttccg ggaatggaaa gcatggagat cctggacgag
     4921 aacaacttcc ggtccttgct ccgggtgaag atgatggggt ccgaatgggt cctgaagctg
     4981 aaaggagaga tggtggacat ctccccccct gaatcttttt ctgtaaggct tcttctcgaa
     5041 ggcctggggg gcctggttaa agctgatcag aaggtgaccc tggccatgac tccggtggaa
     5101 aaaggaaaga cctcggtgac ctgccgggcc acagcggaag acatgggctt tctgcaaagg
     5161 gccttcctta cccgacaggc tcgggttttt gcccagtcca ccttcgaggc catcgagaaa
     5221 cggctgaagg aactggcata accgattgcg gatttcggat tgcggattgc ggaatgaaaa
     5281 acccgtaatg gaagaattag gatgctcgta ggtggcctgt tagaaaaaaa attggttgac
     5341 ggattcaggc ttgtcgattt cagtttaaat tcttttaatc cgaaatccgc attccgcaat
     5401 ccgcaattag gaggataatg atgggtgcga ctcaaattct ggccgattat gtgagcgaga
     5461 cctctttccc cgccttgccc aaagaggtgg tccggaaggc caagggcatc gtcctagaca
     5521 cccttgggtg cgccatcgcc ggctacaccc tggccgctca tgagtttcgc tgggtctacg
     5581 agccggtgaa agagatgggt ggaaatcctg aatccacggt gtggctcgcc gggatgaaga
     5641 cttcctgtcc ccaggcggcc ctggtgaacg ggaccctggt tcatacggta gattttgacg
     5701 acacccacct ggggtccatt gcccacctgg gagcttccgt ggtctcctcc gctctggcca
     5761 tcggggaaaa agtcggggcc gatggacctt cgattctcac ggccatggtc atggcctacg
     5821 aagcggcggg aagaattgga aaagcgatta tgccctccca ctataaatac tggcacccca
     5881 cggggaccct gggaaccatc gcttccgctg tagtcgcttc gaaactcctg ggactcaagg
     5941 cggaccggat ggagcaggcc atcagcctgg cggcggacgg cgcttccggc atgcgctatt
     6001 gcatcgactt cggagatttt tccaagagcc tccatcccgg cttcagcgcc tggaagggga
     6061 tcttgtcggc cctggtaatc gccagcgggg ctgcaggacc caagggactg ctcgaataca
     6121 aatccggctt ctgcgaggct tattccgacg agccagccat gaaacacttg aacgaaagcc
     6181 tcggttcgtc ctacgagatc ctgaccgaca gcctgaaagc ttttcccacc attctctgca
     6241 gccatacccc gatccaggga acggtgaaac tcatgaagga aaagaacctg cgcctggaag
     6301 acatagaagc cattcatttc cgggtgaccc ccaccgcgcc aggacaaggg atgaattatg
     6361 cgcctgatac ccccttggct gcccgcctga gcattcccta ctgcgtcagc cgggccgctg
     6421 cggacggcta catcgccatg gatcaattcc aggaggagaa gatcaaggat gccagggtcc
     6481 agcaattcat gaaaaaagta accctggaat ccgtccaaca attcgtcgaa aaatatccag
     6541 gaaccctggc ggcccaggtt gagattcaaa ccaaggacgg aatgcgattc caggacgaat
     6601 ccatctatcc caaggggcac ccgcagaacc ccatgacgga cgaggagatc aaagataaat
     6661 tccgccgcct ctccttgaac accttggatc gggtgcaaac ggaaaagatc gccgagaaag
     6721 tctacgatct ggagtgggta aaacgtgccg atgaactaat ccggttgctg gtgaaatgaa
     6781 ttttaatccg ggcaagccag aaccaaaatt gtaggggcgg ccccccgtgg ccgcccgcaa
     6841 gatgggcaac cacagggggt tgcccctaca gtctgttacc attttgctca caattttact
     6901 tttgaccccc taatgaccga ttgaaaagga gaaataagtg atccgcaaga ttgcccatat
     6961 cggagtggcc gtgaagaacg tcgaagaggt tgagaaactc tattccgaaa tcctgggcct
     7021 gcccatcagt ggacgggagg tttcgccgga caacaaggtt tcgttcatcc ccttggggga
     7081 tacggccatt gaactcctgg aatccgtgaa gcccgatggg gcaatcgccc gccacatcga
     7141 aaagagagga gaggggatcc accatattgc cctggaagtg cacgacatcg agaaagccat
     7201 ggaagagctg aaagccaaag gtcttgtcct gatcgaccaa aagccgagaa ggggcgccca
     7261 cgattccaag attgcctttc tccaccccaa aggaacctac ggagtgcttc tggagttagt
     7321 ggagccgaag aaacattagg aggtggcggt tcgagaaagc tagaaagaac ccttttcctc
     7381 ctgcgcaaga aaatttcttg acatgggttg cctgaattgg aagaatagcc tcgcaaggag
     7441 aagtccagct aaataggttc cttaaggcaa gttcctgtga gagggaattc ctggaaaata
     7501 atctcttaag gcaaggaggg taaaaaagtg agaagaatat ggaaaggttt tgcagtcgca
     7561 gcgttggcct tcgcgctggc cgtgtggatg agcccgggtc cggtgtcggc cgcggaaaag
     7621 gttttcgaat tcaaaatttc catcgatact gtccccaacc atccgcgata cggggggctg
     7681 gagatcttcg tgccggaact ggccaagagg tccggtggga ggctggtcgg caagatttac
     7741 cacagcgccc agctttacaa ggacgcccac gtgactaaag ccctgaggac cggcacggtt
     7801 gaaatgtgcg tgcccggaaa ctggctgctg gagggattcg ataccagcgc cagccttacc
     7861 atgctgccca tgttcttcgg ccagcccgga gatattaccg agaagctgat cgacgggaaa
     7921 atcggagaag cggtgaaccg gtccctggaa aagaaagtag gagcgaaatc tccgggccgc
     7981 tggtatgagc tgggctatga ccatacctgg acccggaata agaaaataac caaaatcgaa
     8041 gacttcaagg ggttgaagat ccggcactcc ggcggttcca tccccgaaga gcgactcaac
     8101 cgctttgggg ccagcgcggt attcattgcc tggcccgacg tgcccatggc tctggtgcaa
     8161 gggacggtgg acggcgtatg ctccaccacc aagagcgtgg agagcgccaa gctctacgag
     8221 gcaggcatca agtacgcggt cacctgccgc aattacaacg gctactacgt ccccctggtt
     8281 aaccagaaat tctggaacag cctgcccgcc gacctgcaga aaaccttctc ggacgtatgg
     8341 aatgagattg tccccaagca gcgggagatc gcccgcaagg agcaggccga ggccagaaag
     8401 ctcatggagg gcaaaggcgt ggaattcttt gacccgagcg acgaagagct ggccaaatgg
     8461 cg
//
LOCUS       DASPRR010000107         7344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2123136, whole genome shotgun sequence.
ACCESSION   DASPRR010000107 DASPRR010000000
VERSION     DASPRR010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123136"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VEL68_03830"
     CDS             complement(<1..245)
                     /locus_tag="VEL68_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221227.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HXZ35131.1"
                     /translation="MDWGMQNRLGRLIKEDGHCFFLPIDHGYFQGPTSKLEKPGETIK
                     PLIPYADGLFVTRGVLRAAVDPKNCPPIILRVSGGTS"
     gene            437..1477
                     /locus_tag="VEL68_03835"
     CDS             437..1477
                     /locus_tag="VEL68_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HXZ35132.1"
                     /translation="MERSMRILTERPFNAETPAEALRSWITANAVFFHRNQSELKSSV
                     SLPEWRLRIDGEMENPRDFTFDALLCLPKAIAVNTLECSGNGRSFFREKASGNPWTIG
                     GVGNAVWGGIWLKELLQIVEPKKNARHAAFEGMDEPYGAGKLKFIRSIPLEKALTSTL
                     LAYEMNGEPLPLEHGYPLRSLPLGWTGANCTKWLKRISLLHRPYEGYYMDKVYRIYQK
                     GEEPGSGTAVTQISLKSIITQPLPGETLKKKQIVILGAAYGGERTVTKIEVSVDGGRT
                     WAPGEFIGPHEPFSWRQWQYVWKPAKEGAYCLQAKATDAVGNQQPLEARWNVLGYGNN
                     GIREHAIAVFIV"
     gene            1600..2853
                     /locus_tag="VEL68_03840"
     CDS             1600..2853
                     /locus_tag="VEL68_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXZ35133.1"
                     /translation="MHAIEKILAKAAGKDSVVTGEIVNCWVDMGEVNDIYLQTVRSFY
                     EMGAKKVYDPKKLVFIMDHYAPASTIMQADNQKQFREFCREQDIELFFDVDQGVCHQA
                     MVDNGLVYPGMILVATDSHTTTHGAFGAFGTGVGATDMAVIMITGKLWFRVPEIIRFH
                     LTGTLPKGVFAKDAILRIIGDLGADYGIYKGIEFTGPLLKSLNVSERMSLCNMTTEIG
                     AKTAYIQPDDITLNFVRDRVKNEFQVFTTDPGYKYAAEHTYDVSRLKPQLAAPHSVDN
                     VHPLEKFIGTPIDQAFLGTCTGGRAEDLAIAAKILKGRKVHRGTRFIVVPASKQVLLE
                     AMARGSMQTLVEAGATFVTPGCAACLGTHEGILAAGETCITASSRNFPGRMGSTKAQI
                     YVGSPASVAAAAVEGKIADPTGYLD"
     gene            2865..3371
                     /locus_tag="VEL68_03845"
     CDS             2865..3371
                     /locus_tag="VEL68_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016459886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXZ35134.1"
                     /translation="MEKIVKGKAFIFGANIDTDQIYPGRYLELTDPKEVGKHALEGAD
                     PNLVKEFKPGDIIIASTNFGCGSSREQAAITLKAIEVGAILADSFARIFYRNAINLGI
                     PLLVCPKIHDLVKKGDTLRVNLITGEVVNESSGARVQAQPLSDYVMKILESGGIKPLI
                     RKQIGADA"
     gene            complement(3596..4381)
                     /locus_tag="VEL68_03850"
     CDS             complement(3596..4381)
                     /locus_tag="VEL68_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXZ35135.1"
                     /translation="MERMNKFRKVMEEGKIAVGTCSDSYSAAAVEAIGYTSLDFCRID
                     NEYSWRRDEGMENMLRAAAITGITAMVRVEKGAPYLISKIFQAGAGAVLVSDINNYEE
                     ALAVVKASKFAPKGHRGMSTFSFSGGWGTRGGEDFADWSDTQLMVGIMVENEQVVGQL
                     DKIFAIEGLDYALFGPSDYSMSLGFRMTKKNHPKVQDAIKKTCEAANKHKKFVGIGIG
                     EPWAEEAKKYIDMGCRFIEVGHELAILRGVLSSAAAGIRGIKA"
     gene            complement(4420..5415)
                     /locus_tag="VEL68_03855"
     CDS             complement(4420..5415)
                     /locus_tag="VEL68_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXZ35136.1"
                     /translation="MKSERPAYSVVVTGDSLATEAMRLLEGTCRVLFSGPYPDPPKLA
                     QMLRQEKAHALILRTGKAPAEVVKASPELRVISKHGAGYDNIDVATATALRIPVMTST
                     TANYQSVAEHTLGLMLSLAKDITRLDSRIRQGFWDKAQYRGVELFRKTLGLVGFGRIG
                     RRVYELTVPLQMRVLVYDPFLGDRPLPPGVTRLTNLGELLSVADIVSLHCPLTEKTRN
                     LIGEKELERMKKTAWLINTARGGVVDEEALIEALRGGEIAGAAIDTFRQEPPEDLRRL
                     CEAGKVVLTPHIAAATEEAFTRMGVEAAQNVLTILQRKRPDGDRIVNPEVLPQME"
     gene            complement(5452..6426)
                     /locus_tag="VEL68_03860"
     CDS             complement(5452..6426)
                     /locus_tag="VEL68_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ35137.1"
                     /translation="MGKRIFCSLVLLAIAFFFSTPGVHLAAEQKYPTKPIQVIIPFAP
                     GDTDNLLRPFIEKMPEYLGQPLTFVYKPGAAGTLGAGVVASAKPDGYTLGGLSQSALT
                     IVPHVQKDVSFTLQSFAPIACLVESPILLVAKADGPWKNLKDLVSEAKSKPGQINFTT
                     AGTYNIGHIAFEAFAQRAGIKLNFIPSQGSGPSITAVLGGHVHLASAAIVPALPHIQA
                     GTLRALGVYSQNRVATLPDAPTFEELGYPVVIPVYYGFIAPKGTPQGVIEKLHLAAQK
                     VIENHGGFVDERLSKMGARRIFIGTGQYANLLQSQYEYYGELIRGIKP"
     gene            complement(6618..>7344)
                     /locus_tag="VEL68_03865"
     CDS             complement(6618..>7344)
                     /locus_tag="VEL68_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ35138.1"
                     /translation="LRRLRKEMQIIFQDPYASLNPRMTVGQILGEALGIHHNLRGKAA
                     KERMRELLEAVGMSAEHIPRFPHEFSGGQRQRIGVARALAVQPKLIICDEPVSALDVS
                     VQAQVLNLLQDLKRQFQLTYLFIAHDLAVVRHISDRIAVMYVGKIVEMADKDRIYEGA
                     LHPYTRALLQAIPIPDPHLRREYQQLEGELPSPLNLPPGCRFHTRCDRVMEVCRKEEP
                     SFLDLGNGHFAACHLYPEGDLRT"
BASE COUNT         1585 a         2132 c         2029 g         1598 t
ORIGIN      
        1 atactggtcc cgccggagac ccgaaggata atgggtgggc agttcttcgg gtccacggcg
       61 gcccggagga ctccccgggt cacgaagagt ccatcggcgt aagggattaa aggtttgatc
      121 gtctcacccg gtttttccaa cttgcttgtc ggaccctgaa aatatccgtg atcaatgggc
      181 aggaagaagc agtggccatc ctccttgatc agcctaccga ggcgattttg cattccccaa
      241 tccataaaaa cctccttcac caaaagtcat caggcctgat ttcaccgttg gtaatcatag
      301 cattgacccc gaacaaaatt ccaccccaat atggcctgtt ttatcctggc cgactggacg
      361 gattttttct ccttctgctt ttcaccaccc aattctttgt gttatatttt tagcgagccc
      421 ttcagcggga gggtagatgg aacgaagcat gcgcatactg accgaaagac cattcaacgc
      481 cgaaacgccc gcggaagctc tccggtcctg gatcaccgcg aatgcggttt tcttccaccg
      541 caaccagtcg gagctgaagt cctctgtttc gctgcccgaa tggcggctca ggatcgacgg
      601 ggaaatggaa aaccccaggg atttcacctt cgatgcgctt ctctgtctcc ccaaggccat
      661 cgccgtcaac acgctggagt gctcgggaaa tggccgatcc ttctttcggg agaaggccag
      721 cggcaaccct tggacgatcg ggggagtggg gaatgcggtc tgggggggaa tctggctgaa
      781 agaactgctc caaatcgtcg aaccgaaaaa aaatgcccgg catgcggctt tcgaggggat
      841 ggatgaaccg tatggggccg ggaaattgaa attcatccgc agcattcctc tggagaaggc
      901 cctgacttcg accctgttgg catacgaaat gaacggagag cccctgccgt tggagcacgg
      961 ttacccgctc cggtctttgc ccctgggctg gaccggggcg aattgcacca aatggctgaa
     1021 aaggatcagc ctcctccacc gcccctatga gggctattac atggataaag tgtaccgcat
     1081 ctaccaaaag ggcgaggagc cgggatcagg aaccgcggtg acccagattt ccctgaaatc
     1141 catcatcacc cagcctttgc cgggggaaac ccttaaaaag aagcagattg tcattctggg
     1201 ggcagcctac ggaggagaac ggacggtcac caaaatagaa gtttccgtcg acggcggccg
     1261 tacctgggct cccggggaat tcatcggccc ccatgaaccg ttctcctggc gccagtggca
     1321 gtatgtgtgg aaaccggcga aagaaggcgc atattgtctc caggccaagg caacggatgc
     1381 ggtgggcaat caacaacccc tggaggctag atggaatgtg ctggggtatg ggaataacgg
     1441 aattcgggaa cacgccatcg cggtttttat cgtttaggga aaacacatgt cattgcgagc
     1501 gatcccgccc tggcgggaaa gtgcagcaat ctcaccccct ccccaccctc cccctcgagg
     1561 gggaggggga agatgggggg cgatagagtg gaggacatcg tgcatgcaat cgaaaagata
     1621 ttggccaaag cagcggggaa agattcggta gtcaccgggg aaattgtcaa ctgctgggtt
     1681 gacatggggg aggtgaacga catctacctg cagaccgtcc ggtccttcta cgaaatgggc
     1741 gcgaaaaaag tctatgaccc gaagaagctg gtcttcatca tggaccatta cgcccccgct
     1801 tcgaccatca tgcaggcgga caaccagaag cagttccgcg aattctgccg ggagcaggat
     1861 atcgagctct tcttcgatgt ggaccagggg gtctgccatc aggccatggt ggacaacgga
     1921 ttggtatacc cgggaatgat cctggtcgcc actgactccc acacgaccac ccacggggcc
     1981 tttggagctt tcggcaccgg cgtgggagcc accgacatgg ccgtgatcat gatcaccgga
     2041 aagctctggt tccgtgtccc ggagatcatc cggtttcatc taaccgggac gctgcccaag
     2101 ggcgtctttg ccaaagacgc gatcctgaga atcatcggcg acctgggagc ggactacgga
     2161 atttacaaag ggatcgaatt caccgggccg cttttgaaga gtctgaatgt atccgagcgc
     2221 atgtcccttt gcaacatgac cacggagatc ggggcaaaaa ccgcttacat ccagcccgac
     2281 gatattaccc taaatttcgt gcgcgaccgc gtgaaaaatg aattccaggt attcaccacc
     2341 gacccggggt acaagtatgc cgccgagcac acctatgacg tctcccgttt gaaaccccag
     2401 ctggccgcgc cccacagcgt ggacaacgtt catcccctgg aaaagttcat cggtactccc
     2461 atcgaccagg cctttctggg gacctgcacc gggggacggg cggaagacct ggccattgcg
     2521 gcaaagattt tgaaagggag aaaggttcac cggggaaccc gtttcatcgt tgtccccgct
     2581 tccaagcaag ttctcctgga ggccatggcc agaggatcca tgcagaccct ggtggaggcc
     2641 ggagccacct tcgtgacccc cggctgcgcc gcctgcctgg gcacgcatga ggggattttg
     2701 gctgcggggg aaacctgcat caccgcctcc agcaggaatt ttccggggcg catggggtcc
     2761 acaaaagcgc agatttacgt gggctcgccc gcgtcggtgg ccgctgccgc cgtggagggg
     2821 aaaattgccg atccgaccgg atacctggat taaaggggga aaacatggaa aaaatcgtaa
     2881 aggggaaggc ctttattttt ggggccaaca tcgatacgga ccagatttat cccggcaggt
     2941 atctggaatt gaccgacccg aaggaggtgg ggaagcacgc cctggaggga gcggacccga
     3001 acctggtaaa agaatttaag cctggggata tcatcattgc ctccaccaac tttggctgcg
     3061 gctccagccg ggaacaggcg gccatcactt taaaagccat tgaggtcggg gcgatccttg
     3121 ccgattcttt tgcccgcatc ttctaccgca atgccatcaa cctggggatt cccctcctgg
     3181 tctgcccaaa aatccacgat ttggtgaaga agggggacac cctccgggtc aatttgatca
     3241 ccggggaagt ggtcaacgag tcatccgggg ctagagtcca ggcccaaccc ctatccgact
     3301 atgtcatgaa gatcctggaa agcggaggaa taaaaccctt aattcgaaaa cagatcggag
     3361 cggatgcctg aatcctcgac ggcaggggct gttcttgaat ccgcccctgc cattcagcag
     3421 aaaaatcgct tcatttgagc ctcttgcaaa agtcacttac tccgtcattc cggcgaaagc
     3481 cggaatccag agttctttga gaatcctgga ttccgggtcg cccccggcct tcgccggggt
     3541 tgcccggaat gacaacttcc gcttgttttc aagagtttcg aaagaggcta attctctacg
     3601 cttttatccc ccggattccc gcagccgccg aactcaatac ccctctgaga atggccagct
     3661 cgtggcccac ctcgatgaac cggcagccca tgtcgatgta tttcttggcc tcctccgccc
     3721 agggctctcc aatcccgatg cctacgaatt ttttatgctt gtttgccgcc tcgcaggtct
     3781 tcttgatggc atcctgaacc ttgggatggt ttttcttcgt catgcggaag ccgagggaca
     3841 tgctgtaatc cgacgggccg aaaagagcgt agtccaatcc ctcaatggcg aagatcttgt
     3901 cgagctgccc cacgacctgc tcattttcca ccatgattcc caccatcaac tgggtgtcgc
     3961 tccaatccgc aaagtcctcc cccccgcggg ttccccagcc cccggaaaag gaaaaggtgc
     4021 tcattccccg gtggcccttg ggggcgaact tcgaagcctt gaccactgcc agggcctctt
     4081 catagttgtt aatgtccgaa accagtacgg cgcccgcgcc cgcctggaag attttggaaa
     4141 tgaggtaggg ggcccctttt tccacccgga ccatggccgt gatccccgtg atggctgccg
     4201 ccctcaacat gttctccatc ccctcgtccc tccgccagga gtactcgttg tcgatcctgc
     4261 agaagtccag gctggtgtag ccgatcgctt ccacggcggc ggcgctgtag gaatcggaac
     4321 aggtccccac ggcgattttt ccttcctcca tgactttcct aaacttgttc attctttcca
     4381 tttttttcct cctgggtttg ctgaaaattg ccgtctacat cattccatct gcggcagcac
     4441 ttccggattc acaatccgat ctccatccgg ccttttcctc tgaagaatgg tcaaaacgtt
     4501 ctgggccgct tccaccccca tgcgggtgaa ggcctcctcg gtagccgcgg cgatgtgcgg
     4561 agtcaaaacc accttacccg cctcgcaaag gcgccgaagg tcttccggag gttcctggcg
     4621 gaaggtatcg atcgccgccc ctgcgatctc tcctccccgg agcgcctcga tgagcgcctc
     4681 ttcatccacc acccccccgc gggccgtgtt gatcaaccag gcggttttct tcatcctttc
     4741 cagttctttc tccccgatca agttccgggt tttctccgtg agggggcagt ggaggctcac
     4801 gatgtcggcg acggacagta actctccgag gttcgtaagc cgggtaactc ctggcggaag
     4861 gggtcgatct cccaggaatg gatcatagac caacactctc atctgcaatg gcacggtcaa
     4921 ttcatacacc cgccggccga tccgcccgaa cccgactagc ccgagggttt tccgaaacaa
     4981 ttcgactccc cggtattgcg ccttgtccca aaagccctgg cgtatccggc tgtccagccg
     5041 tgtgatatct ttggccaggc taagcattaa gcccagggtg tgttcggcca cggactggta
     5101 attggctgtg gtcgaagtca tgaccggaat ccttaaagcg gtcgcggtgg ccacatcgat
     5161 gttgtcgtac ccggctccgt gtttggaaat taccctcagc tccggggaag ccttgacgac
     5221 ctcggccggg gctttccccg tccggagaat caaggcatgg gctttctcct ggcggagcat
     5281 ctgggcgagt ttgggcgggt ccggataagg gccggaaaaa agaacccggc aggttccttc
     5341 gagaagtctc attgcttccg ttgccagaga atcgccggtt acaaccacac tgtaagctgg
     5401 tctttctgat ttcatccttt acctcttttt atatcgccga ttcctgattg cctatggctt
     5461 gatcccccgg attaactctc cgtaatattc atactggctc tgcaggagat tcgcgtattg
     5521 accggttcct ataaaaattc ttcgcgcgcc cattttgctc aaccgttcat ccacgaaccc
     5581 cccgtggttc tcaatgacct tctgggcggc cagatgcagt ttttcaatca ccccttgcgg
     5641 tgttcccttg ggggcgataa atccataata gacggggatg accaccgggt atcccagctc
     5701 ctcaaaggtg ggggcatccg gaagggttgc aacccggttt tgcgaatata cacccagggc
     5761 ccgcaaagtt cccgcctgga tatgggggag agccggaacg atggctgccg aggccagatg
     5821 aacatgcccg cctaagaccg cggtaatcga aggcccgctc ccctgggagg ggatgaagtt
     5881 caacttgatt cccgctcttt gggcgaaggc ttcaaaagca atgtggccga tattataagt
     5941 cccggccgtc gtaaagttga tctgacccgg cttgcttttg gcttctgaca ccaggtcttt
     6001 aaggtttttc cacggaccgt cggccttcgc cacgagtagg atggggcttt ccaccaggca
     6061 ggcaatcggg gcaaaggatt gcagggtaaa ggacacgtcc ttttggacat gaggaacaat
     6121 ggtcaaggcc gactgagaaa gccctccgag agtatacccg tcgggcttgg ccgaagccac
     6181 aacgcccgcc cctagggtgc ccgcggcccc cggcttgtac acgaaggtca gcggctggcc
     6241 caaatattcg ggcatttttt cgatgaaagg ccggagaaga ttgtccgtat ctccgggagc
     6301 aaagggaatg atgacctgga tgggtttggt aggatatttt tgctcagccg cgagatgaac
     6361 tcccggggtt gaaaaaaaga aggcgatggc caacaaaacc aggctgcaaa atatccgctt
     6421 ccccatgatc aaacctcctc cctcaaaatt tttcccaaat atccactcgt tcggctttct
     6481 tccaatagat cgattgttga caaaatgagt tatgcaccct aagaatcaac gtcccttacg
     6541 tcttcccttc actggaagaa atgagtggag gaaatcctcc ccgctggaat cgctctgtct
     6601 gccccgccct tgcggcttca ggttctcagg tctccctctg gatacagatg acaggccgca
     6661 aaatggccgt ttcccaggtc aaggaaagag ggctcctctt tccgacagac ctccatcacc
     6721 cggtcgcaac gggtgtggaa cctgcaaccg ggtgggaggt tcagcgggct ggggagttcg
     6781 ccttccagct gctggtactc tctccggaga tgtgggtcgg ggataggaat ggcctgcagg
     6841 agggcccggg tgtagggatg cagcgctccc tcgtatatcc tatccttatc cgccatctcc
     6901 acgattttcc ccacatacat gaccgcgatc cggtcgctga tgtggcggac gacggccagg
     6961 tcgtgggcga tgaagaggta ggtcaactga aactgtcgct tcaaatcctg cagcagattg
     7021 agaacctggg cctggaccga gacatccaga gcggaaaccg gctcgtcgca gatgattagc
     7081 ttgggctgca cggccagggc ccgagccacg ccgatccgct gtcgctgtcc tccgctgaac
     7141 tcatggggaa aacgggggat gtgctcggcg ctcattccta cggcttccag cagttccctc
     7201 attcgctctt tggccgcctt tcctctgagg ttgtgatgaa ttcctagggc ttctccgaga
     7261 atctgcccga ccgtcatgcg ggggttcaag gaagcatagg ggtcctggaa gatgatctgc
     7321 atttctttgc gcagccgtcg aagc
//
LOCUS       DASPRR010000108         6389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_993335, whole genome shotgun sequence.
ACCESSION   DASPRR010000108 DASPRR010000000
VERSION     DASPRR010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993335"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /gene="mtaB"
                     /locus_tag="VEL68_03870"
     CDS             complement(<1..1085)
                     /gene="mtaB"
                     /locus_tag="VEL68_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HXZ35139.1"
                     /translation="MDRKSPLKIALITLGCKTNQSDAASLAAELLMQGHRIVPSSQTA
                     DVIIIHTCTVTQKTDYQSRQIIRRMAAKNPRGQVVVTGCYAQVSPESLQAIPGVDYVV
                     GMAERKRIPGIIIAKEKSRKAAVLISPVEKQKSFEEWMLPHFHDRTRAFLKVQDGCNS
                     YCSYCIVPYARGRNRSLPLERVLFQARGLAAQGFKEIVLTGIHLGTYGEDLLPPPSLL
                     ILLKELERQNLSLRLRLSSIEPREFNPPLMEYLASSRRVCPHLHIPLQSGDDRILRRM
                     NRNYSSAFFADLVNRLAQTIPGAAIGADVIGGFPGEDDSAFLNTMTLIENLPLAYLHV
                     FPFSKRKGTPASAFPGQVPPQVILARC"
     gene            complement(1064..2206)
                     /gene="mnmA"
                     /locus_tag="VEL68_03875"
     CDS             complement(1064..2206)
                     /gene="mnmA"
                     /locus_tag="VEL68_03875"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXZ35140.1"
                     /translation="MAVLHNLGGKGLLNKKERIVVAMSGGVDSSTAAALLKEKGYEVI
                     GLTMHLWDPARHGKGPFGRCCSLDDIHDARRVADQIGIPHYVVNLKQAFEERVVENFV
                     EEYLRGRTPNPCVHCNDQIKFGFLLHKAEELGAKALATGHYARIVQASTFDEAREARF
                     LLLRGKDRNKDQSYFLFLLTQEQMGKVLFPLGDKSKAEVRQVAAKLGLRVALKEESQE
                     VCFVPDNDYRRFLEDRKGKEISKLGEIVNRRGEVLGSHPGIYSYTIGQRRGLGISAPQ
                     PYYVLALDCQLNRVIAGKKEELLAHGLIAGGINWIAVAPLEGETEALVQIRYRHPGVP
                     AVLSAREDKKVSVQFKIPQKSVTPGQAAVFYRGDEVLGGGWIERAL"
     gene            complement(2245..3423)
                     /gene="nifS"
                     /locus_tag="VEL68_03880"
     CDS             complement(2245..3423)
                     /gene="nifS"
                     /locus_tag="VEL68_03880"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HXZ35141.1"
                     /translation="MRRVYMDHNATTPVHPEVLEAMMPFFRDQFGNASSIHWAGREVK
                     KYLDEARENVAALMNADPQEIIFTGCGTESDNMAIKGVGSTHLDKGRHIITSKVEHHA
                     VLHTCQFMEGLGYQITYLSVDREGLIDLEDLRRSIRPDTVLVTIMFANNETGTIMPVQ
                     EIGEVCREKGVIFHTDAVQAVGKIPIDLKKLPVDILSLSGHKLNTPKGIGAQFIRKGT
                     KLSPLIHGGAQERNRRAGTENIPYIIGLGKACEIAKRDSQKRHEYLRSMRDRLQEGLL
                     RAIPHVELNGHPTRRLPNTLNASFLYVEGESLLLNMDLEGIAVSSGSACSSGSADASH
                     VILAMGKSPLVAQSAIRFSLGLTNTAEDVDYVLEVVPRIIDRLRAISPLYQKVSPSRV
                     "
     gene            complement(3750..4160)
                     /locus_tag="VEL68_03885"
     CDS             complement(3750..4160)
                     /locus_tag="VEL68_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HXZ35142.1"
                     /translation="MTSKKIVLHFSKENWNKPIVYRLVKDFNLSFNILKANVLPKQES
                     FMVLELSGPKADFKRGMDYLLGSGIVVEPIEKDIGRDDLKCIHCGACTAVCPTGSLYI
                     NRKTMEVIFDTSKCSGCELCIKACPPRAMESRLV"
     gene            complement(4399..5760)
                     /locus_tag="VEL68_03890"
     CDS             complement(4399..5760)
                     /locus_tag="VEL68_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXZ35143.1"
                     /translation="MMAADAGIGLRYPVPWDQVEKETLEHFQTIVRMDTSSPPGNETL
                     VAEYLKQVLEREGISGRLLALDPKRANLVARIRGKGSKRPILVMGHTDVVGVQREKWS
                     VDPFGAVRKDGYIWGRGTGDDKDNVTAGLMLLLLLKRQGVKLDREVVFLAEAGEEGYN
                     REGLRYVIANHWDEIDAEFALAEGGGGIIKDGKVLYLSVATSEKVRIEVQLLARGTAG
                     HGSIPRPDNAVVRLANAISRVAEWAQPMRLDETTRTYFQRLAGISPPEAAARYRAILT
                     SNERAAAERYFAQHELRHNSMIRTSLSPNVLKAGFRANVIPSEAEALIDIRALPDEDL
                     QKFVERLREVIEDSHIEIKVNSGRPAAQASSLNTEMFAALEAAQRRLYPESITIPILL
                     PGATDMSPLRAKGIQAYGIGPVVDSDETGGAHSDDERILEKSLHDFVRFIWHAILEVA
                     AAK"
     gene            complement(5966..>6389)
                     /locus_tag="VEL68_03895"
     CDS             complement(5966..>6389)
                     /locus_tag="VEL68_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ35144.1"
                     /translation="SEVVISDEEISFPMVTHPELFIALSQEALDRYIGDLPEGKTLIV
                     DEDLVKNIPERKGITIYRLPMTRVADKKLKNRMFTNIVMIGAVTDFTGLVSREAMRQA
                     VKNIVPPATIEKNLTALDAGFALAEENRGGRTPRGKAT"
BASE COUNT         1320 a         1771 c         1763 g         1535 t
ORIGIN      
        1 tggcagcggg ccaggatgac ctgcggcggg acctgcccgg gaaaagcaga ggccggggtt
       61 cccttcctct ttgaaaaggg aaagacatgg agataggcaa ggggaaggtt ttcaatcagg
      121 gtcatagtat tcagaaaggc agaatcatct tcccccggga agcctccgat gacatcagct
      181 ccgatcgccg caccgggaat ggtttgggcc agacggttca ccaagtcagc aaaaaaagcg
      241 cttgagtaat ttcggttcat ccgccttaga atgcggtcgt ccccgctctg cagggggata
      301 tgcaggtggg gacagacccg gcgggaggag gctaggtatt ccatcagtgg agggttgaac
      361 tcccttggtt caatggagct gaggcgaagg cgaagactca ggttttgcct ctccagctcc
      421 ttcaagagga tcagcaggga tgggggagga agaaggtcct ccccgtaagt gcccagatga
      481 attcccgtga ggacgatttc cttgaaccct tgggcggcta atccccgggc ttggaacagg
      541 acccgctcca ggggaagact cctgttcctc ccccgggcat aagggactat gcagtaggaa
      601 caataggagt tgcagccatc ctgaactttg aggaaagccc gggttcgatc gtgaaaatga
      661 ggcagcatcc actcttcaaa acttttctgc ttttccaccg gagagattag aacagccgct
      721 ttccgggatt tttccttggc aatgatgatt cccggaattc tcttcctctc cgccattccc
      781 acgacataat ccacccctgg aatagcttgc agggattcgg gcgaaacctg ggcgtagcat
      841 ccggtaacga cgacctgccc tctgggattc ttggctgcca tgcgacggat gatctgccgc
      901 gattgatagt ccgtcttctg ggttacagtg caagtatgaa tgatgattac atccgcagtc
      961 tgagaagaag gaacgatccg atgcccctgc atcaagagtt ctgcagccag ggaggcggcg
     1021 tcactctggt tggttttgca gcccagagtg atcagggcga tctttaaagg gctctttcga
     1081 tccatcctcc acccaagact tcatcccccc ggtaaaacac cgccgcctgc ccaggggtta
     1141 cggacttttg gggaatcttg aattggacgc ttactttttt atcttcccga gcagaaagga
     1201 ctgcggggac gcccggatgc cggtatcgga tttgaaccag ggcttccgtc tctccttcca
     1261 gaggagcaac ggcaatccag ttgattcccc cagcgatcag gccgtgggcc aggagttctt
     1321 ccttttttcc cgctatgact cggtttagct ggcaatccag agccagaacg taatagggct
     1381 ggggggcgga aatgcccaat ccccggcgct gcccgatggt gtaagaatat atgcccgggt
     1441 gggagcccag tacttctcct cgccgattca cgatctctcc cagtttggag atttctttgc
     1501 cctttcgatc ttcgagaaat cgacggtaat cattgtccgg gacgaaacaa acctcctggc
     1561 tttcctcctt cagggccacc cgtaaaccca gttttgcggc aacctgccgc acttccgctt
     1621 tggatttatc ccccaagggg aagaggacct tgcccatctg ttcctgggtc agaaggaaga
     1681 ggaaatagga ctgatccttg ttgcggtcct ttccccgcag gagaagaaac ctcgcctctc
     1741 tggcctcgtc gaaagtagag gcctgcacga tcctggcgta atgtcccgtg gccagggcct
     1801 tggcccccaa ttcttcggct ttgtgaagta ggaagccgaa cttgatctgg tcgttgcagt
     1861 gaacgcaggg attgggggtt ctccctcgca ggtactcctc gacgaagttt tcaaccactc
     1921 tttcttcgaa agcctgcttc aggttgacca cgtagtgagg gatcccgatc tggtctgcta
     1981 cccgccgggc gtcgtggatg tcgtcgaggg agcaacagcg gccgaagggg cccttcccgt
     2041 gccgggccgg gtcccatagg tgcatggtca ggccgatcac ctcgtagcct ttttctttca
     2101 aaagtgctgc ggccgtggaa ctgtccaccc ccccgctcat ggctacgaca attcgttctt
     2161 tcttgttcaa aagacctttc ccccccagat tatgcagcac ggccatgtcg gtctgtggtc
     2221 tctcggcctc tagggtgaac ggattcagac tcttgacgga gatacttttt ggtacagggg
     2281 agagatcgcc cgaagccggt cgatgatccg cggcacaacc tccagcacgt agtccacatc
     2341 ctcggcggta ttggtcaacc ccaggctgaa acggatggcg ctctgggcca cgagggggga
     2401 tttccccatg gccagaatga catgagacgc gtcggccgaa ccggaggaac aggcggaccc
     2461 tgacgatacg gcgatccctt ccaggtccat gttcaataat aaggattcgc cttccacata
     2521 aagaaaactg gcgttcagcg tattcggcag ccgccgggtg gggtggccat ttaattcgac
     2581 atgggggatc gcgcgaagga ggccttcttg gagccggtct ctcatgctcc ggagatattc
     2641 atgccttttt tgggaatccc gcttggcaat ttcgcaggcc ttgcccagac cgatgatata
     2701 aggaatattc tccgtccctg cccggcggtt gcgctcctgg gctcctccat ggattagggg
     2761 agaaagcttc gtccccttgc ggatgaattg ggccccgatt cctttgggag tgttcagctt
     2821 gtggccggag agggagagga tgtccacggg cagctttttc aagtcgattg gaatctttcc
     2881 tacggcctgc accgcgtcag tatgaaaaat gacccctttc tcccggcaga cctccccgat
     2941 ttcttggacg ggcatgatcg ttccggtctc gttgttggcg aacatgatgg tgaccaggac
     3001 ggtatccggc cggatcgagc ggcgaaggtc ttccaggtcg atcaatcctt cccggtcaac
     3061 ggaaagatag gtgatttggt agcccaaccc ttccatgaat tggcaggtat ggaggaccgc
     3121 atggtgttcc accttggagg tgatgatgtg ccgacctttg tccagatggg tggagccaac
     3181 ccctttgatg gccatgttgt cgctctccgt gccgcatccg gtaaagatga tctcttgggg
     3241 gtcggcgttc atcaacgcag ctacgttctc tcgggcttcg tccaggtatt tcttgacctc
     3301 tctgccggcc caatggatac tggacgcatt tccgaattga tcccggaaga aaggcatcat
     3361 ggcctccagg acctcgggat gtaccggggt cgtggcattg tgatccatgt aaacacggcg
     3421 catggtattg ctcctctatt tcttgttaaa ggggttaccc ttcttgcccc aactcctctt
     3481 ccgcatggct tgagctggag tatctccttc tcccccgaat cgtggaagat gggagggtga
     3541 gggggcaaag tctttataaa taattctgat ctctgaagac caaaaggaaa aattactcga
     3601 gaatcaaaag gagaatttgt ggaaagggga atttaccccc ccttcaatta cctgccaggg
     3661 gaggacgatt ctccagccca caatttcttt ttcgtatctc ttaaatcttc ttacgttctg
     3721 cgaaatctgc ggataaacag ttttcggtat tacaccaaac gggattccat ggctctcggc
     3781 ggacaggctt taatgcacag ctcgcatccg ctgcacttgg aggtgtcaaa aatgacttcc
     3841 atagtcttgc gattgatata gagagagccc gtggggcaca cggccgtgca ggctccacag
     3901 tggatgcatt tgaggtcgtc ccggccgata tccttttcga tgggttcaac gacgatcccc
     3961 gatccgagga gataatccat cccccgcttg aaatccgcct tcggaccgct cagctccaga
     4021 accatgaagc tctcttgttt tggcaaaaca ttggccttga ggatgttgaa actcaagttg
     4081 aaatctttga ccagccggta cacaatcggc ttgttccaat tctccttgga aaaatggagg
     4141 acgatcttct tggaagtcat ggctcttctc ctgagaggtt cctcctttaa acctagaata
     4201 cttaatcatt tttgtcaact attatgaact attatgaaaa tttatgaata ttttagccgg
     4261 acttcctatt gaagcttccc cctctcgtgg cggggggctg gagtgagggg gaatatcagc
     4321 agaaagcttt agccacctcg atgaattttg gatggttcaa aaacccgctt aaaggcagaa
     4381 tttttggaac agggggtgct actttgccgc cgccacctcc aagatcgcat gccagataaa
     4441 ccgcacaaaa tcatggagcg atttttccag gatgcgctca tcgtccgaat gggcaccgcc
     4501 ggtttcatcg ctgtccacga ccggcccgat gccgtaggcc tgtatgccct tggcgcgaag
     4561 cggactcatg tcggtggctc cggggagaag gatcgggatg gttatgcttt ctggataaag
     4621 ccgtctttgc gcagcttcca gcgcggcgaa catttccgta ttcaaacttg aggcctgggc
     4681 cgcaggccgc ccggagttga ccttaatttc gatatgggag tcttcgatga cctccctcaa
     4741 gcgctccaca aacttctgta ggtcctcatc cggaagggcc cggatgtcga tcagggcttc
     4801 ggcctccgag ggaatgacat tggcgcggaa tccggccttc agtacattgg gggagaggga
     4861 tgttcggatc atggaattgt gtcgcagctc atgctgggcg aaataacgtt ctgccgcggc
     4921 ccgttcattt gaggtgagga tcgcccgata gcgggctgcc gcttcgggcg ggctgatccc
     4981 cgcaagccgc tggaaatagg ttcgggtggt ctcgtccagc ctcatgggct gtgcccactc
     5041 ggcgacccgg gagatggcgt tagccaggcg gaccacggca ttatcaggcc tcgggattga
     5101 tccgtgcccg gccgtgcccc gggcaagcag ctgcacttca atgcgaactt tctcacttgt
     5161 agccacgctg agatagagga ctttgccgtc tttaatgatc ccgccgcccc cctcggccag
     5221 ggcgaattcg gcatcgatct cgtcccagtg attggcgatg acatacctca gcccttcccg
     5281 gttgtatcct tcttctccag cctcggccag aaagaccacc tcccggtcga gtttcacccc
     5341 ctggcgcttg aggagaagca ggagcatcag cccggcggtc acattgtcct tgtcatcgcc
     5401 cgtcccccgc ccccaaatat atccatcttt gcgcacggcg ccgaagggat cgacggacca
     5461 cttctcgcgt tggaccccga ccacgtcggt atgccccatc accagtatgg gccttttaga
     5521 cccttttcct cggatgcggg ccaccaggtt ggcccgcttc gggtccaggg ccagcaaccg
     5581 gcctgaaatt ccttcccgtt ccagaacctg tttcaaatac tccgccacca gcgtttcatt
     5641 ccccggaggg ctgcttgtat ccatgcgaac gatggtctga aagtgctcga gcgtctcctt
     5701 ctccacctga tcccagggaa ccggatacct caaaccgatt ccagcgtctg ctgccatcat
     5761 tttgattttc ctccatcagc agggataacc ccgcgcctct tatgcgtccg gcggtaaatt
     5821 gccattttct tggaaatctg tcaagggttt gaggacaggg tttgaggatg aaatggatcg
     5881 ctctgaacta gggctgtcag caggaattcc ctgcgggggg tgaattcgtc ctatggaaat
     5941 ggaaataagt aattatttga atttttcagg ttgccttccc cctgggggtc cttccgcccc
     6001 tgttttcttc cgccagcgca aagccagcgt ccagcgcggt gaggtttttt tctatggtgg
     6061 cggggggaac gatatttttc acagcctgcc gcatcgcttc ccgcgagacg agaccggtga
     6121 agtcggtgac cgcgccgatc atcacgatgt ttgtaaacat acggttcttc aacttcttgt
     6181 cggcgacccg ggtcatcgga agacggtaga tcgtgatccc ttttctttcc ggaatattct
     6241 tgaccaggtc ctcgtccacg ataagggttt ttccctccgg caggtccccg atatacctgt
     6301 ccagggcctc ctgggagagg gcgatgaaga gttccggatg ggtcaccatg gggaaagaga
     6361 tctcttcatc ggagatgacg acctcgctt
//
LOCUS       DASPRR010000109         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1803047, whole genome shotgun sequence.
ACCESSION   DASPRR010000109 DASPRR010000000
VERSION     DASPRR010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803047"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1371
                     /locus_tag="VEL68_03900"
     CDS             100..1371
                     /locus_tag="VEL68_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35145.1"
                     /translation="MEKDRRKLILSWCLYDWANSAFATTIMAAVLPVFYSSIAGANLS
                     KTMASSYWGYTNTIAMLIVASTAPILGALADHSGTKKRFLALFAGMGAISTTMLVFVG
                     RGDWFLASLLYIIGMVGFSGGNNFYDSLLPHVAGEKDIDRISSYGYALGYLGGGILLA
                     FNLAMILKPHWFGIPDAEWGTRCSFLTVGVWWIIFSIPILRNVPEPPIVPIKGESTHP
                     LRASIQRLSQTFHNLRQYREAFKFLIAFWLYNDGIGTIISMAVIFGAEINIPQAHLIG
                     SILAVQFIGIPFSILFGRLAGWMGTKRAIFLGLAVYTGISLGGYFIQTPLHFWILAVL
                     VGFVQGGTQALSRSLFGTLVPKSRSAEFFSFLDVSSKFAGIIGPFVFGIVGQMTGSSR
                     LSVVALVLFFIGGGALLLTVNEKEGRARAFS"
     gene            complement(1473..1661)
                     /locus_tag="VEL68_03905"
     CDS             complement(1473..1661)
                     /locus_tag="VEL68_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35146.1"
                     /translation="MSSILPEGTSVRKAVQWISKEREEGKTPLPTLVEQACVRFDLSP
                     KDAEFLSRFFTEGTAAKK"
     gene            complement(1754..>2051)
                     /locus_tag="VEL68_03910"
     CDS             complement(1754..>2051)
                     /locus_tag="VEL68_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXZ35147.1"
                     /translation="ETEVQKKRVEKLKKMSPADIAPLVAYLASDEAQYVSGQVFGVRG
                     KEIFIFSQPRPVRSIHHSEGWTPDRLSEMFKGSLGMHLVPLETSGQVFSYDPLV"
BASE COUNT          430 a          552 c          547 g          522 t
ORIGIN      
        1 tcttccattc atgcatggct tcagtataga ggaaagggaa atagctggca aggagaatag
       61 aatgcggatt tcaggcctca taaagagaga aagttggtta tggaaaaaga ccggcggaaa
      121 ttgatcctta gctggtgtct ctatgactgg gcgaattccg cctttgccac caccatcatg
      181 gctgccgttc tcccggtgtt ctacagttcg attgccgggg ccaacctttc caagaccatg
      241 gccagcagct actggggtta caccaacacc atcgccatgc ttatcgtggc ctccaccgct
      301 cccatcctag gggccctggc cgaccacagc gggacaaaaa aaagatttct ggctctcttt
      361 gccgggatgg gcgccatctc cacaaccatg ctcgtattcg tgggtagagg agattggttt
      421 ctcgcctccc ttctctacat catcggcatg gttggttttt caggaggaaa caacttctat
      481 gattccctgc tgcctcacgt ggccggggaa aaggatatcg accgcatttc ctcttacggg
      541 tatgccctgg ggtatctggg aggagggatc ctgttggcct tcaacctggc catgatcctg
      601 aaaccccatt ggttcggaat ccccgacgcc gaatggggaa cccgctgctc ctttctaacc
      661 gtgggggttt ggtggattat tttttccata ccgatcctta gaaatgtccc cgagccgccg
      721 attgttccca tcaagggaga gagcacccat cctctccggg ccagcattca acgtctatcc
      781 cagacctttc acaaccttcg gcagtaccgg gaggccttca aattcctgat cgccttctgg
      841 ctttacaacg acgggattgg aacgatcatc agcatggcag tgatcttcgg ggcggagatc
      901 aacattcccc aagcccacct gatcgggtcc attctggcgg tgcagttcat cgggattcct
      961 ttctcgattc tgttcgggcg gctggcggga tggatgggga cgaagcgggc gatctttctg
     1021 gggttggcgg tttacaccgg catctcgctc gggggttatt ttatccaaac ccccctccat
     1081 ttctggattt tggccgttct ggtggggttc gttcagggtg gaacccaggc cttgagccgg
     1141 tcccttttcg gcaccctggt tccgaaaagc cgaagcgcgg agttcttctc ctttttagac
     1201 gtgagttcca aatttgccgg gatcattggg cccttcgtct ttggaatcgt ggggcagatg
     1261 accgggtcca gccgcctgag tgttgtggcc ctggtgcttt ttttcatcgg tggaggggcc
     1321 cttcttctta cggtgaatga aaaagagggc agggccagag cattttccta agaaagggca
     1381 tgatccgcgg gttgggggaa catatccccc atttctggct aaaccgattc atttcgtcga
     1441 tattttatca acggtacctc taggaccaca ccctactttt ttgcggctgt tccttcagtg
     1501 aagaagcggc ttaaaaattc ggcgtctttg ggggaaaggt cgaaacgcac gcaggcctgt
     1561 tccaccagag tcggaagagg ggtctttcct tcctctcgct ccttggatat ccattgaacg
     1621 gctttccgca cgcttgtccc ttcaggcaaa atggatgaca tccgatacct cctcaagaaa
     1681 agttctgcaa ggggcaaaga aagatcgtct cgtaaaaaat ctgaacccct tttttcggtt
     1741 atggagtcat aactcaaacc agaggatcgt agctaaaaac ctgtccggac gtttccagag
     1801 gaaccagatg catgcccagg gatcctttga acatttcaga aagacgatcc ggggtccatc
     1861 cttcggagtg atggatggag cggaccggcc tgggctggga gaagatgaaa atctctttgc
     1921 cgcgcacacc gaaaacctga ccggacacgt actgggcctc atcgctggcc aggtatgcca
     1981 ccaggggtgc gatatccgcg gggctcatct tcttcaattt ttccacccgt ttcttttgga
     2041 cttccgtttc c
//
LOCUS       DASPRR010000110         6534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_513276, whole genome shotgun sequence.
ACCESSION   DASPRR010000110 DASPRR010000000
VERSION     DASPRR010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513276"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VEL68_03915"
     CDS             complement(<1..866)
                     /locus_tag="VEL68_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXZ35148.1"
                     /translation="MLHPSQGIVAKAEAPAQLISRKAGWAEEDPEQWWQNVAEVTRIC
                     LEKAGIARKDVGAVGVSGMVPTLILLDRFGKVLRPSIQQNDARSFEEIIEFRQIVDEE
                     NVLQRTGSAITQQSIGPKLLWLRRHEPDAMDQAAHLMGSYEYIVYRLTGSFSCERNWA
                     LESGLFDLHREDWDESLLKLSTISHDWLGKVSWSSEVVGGLNGKAALHTGLAEGTPVV
                     TGSADHIASAFSAGVKSPGDLLVKLGGAGDILYGLDRLVVDPRLFLDYHVIPGKYLIN
                     GCMAASGSIIKW"
     gene            complement(961..1782)
                     /locus_tag="VEL68_03920"
     CDS             complement(961..1782)
                     /locus_tag="VEL68_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HXZ35149.1"
                     /translation="MSDLLQEMFGVPKPVIAMAHFPALPGTPRYDASRGVEGILDRMR
                     QDVIHLLNGGVDGILFCNEDDRPYSFKADLEAVAVMARVIAELKPRDIPFGVDFLWDA
                     RAPLAVAKATGAAFIREVITGVYESDMGIWAPNPAAIFRYRKQIDAENVKVFGNITPE
                     FASPLGNRSVGQRAKSAVVSSLLDVVLISGSMAGSEPDLSWLKEAKEAVGPTIPVLLN
                     TGARAENIQKFLTVADGVIVGSSLKEKGYTWNPVDPARVRAFMEAVSAVRKGGRA"
     gene            1959..2969
                     /locus_tag="VEL68_03925"
     CDS             1959..2969
                     /locus_tag="VEL68_03925"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HXZ35150.1"
                     /translation="MGEDDLRVTKPEDFRDYWDKVRRIVYDTDPEVSVEKWEQEDVNF
                     EGEYILDGPVQKQAKQPESLNPFDFRWDSHVILAGLTVKKVLFRSYDGQEVGGLLQVP
                     RFSGMDRFPMLVHYAGYGGEILIDSDFVTAGYAVFNFSHRGMLMGSKGFDRYHPTPLL
                     VQHVADKDRYIYRSIVIDCLLAIKVMAKTEKVDPCRLGVMGTSQGGALAIIAASLNPQ
                     VKAAACDLPWLTDYDYILNHDVGGLYNELKEFLRRNPEKRKEVARTLGYFDTLSFADG
                     VKGPIFMSLGESDPIAPAVSVRKLFQNIPGIKTLLETPGMGHERSTLFRYLAQKWFDF
                     FL"
     gene            3621..5036
                     /locus_tag="VEL68_03930"
     CDS             3621..5036
                     /locus_tag="VEL68_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXZ35151.1"
                     /translation="MGKRKIYRLFWLGFFLAILSSLSCAHYPPNHVLTQVSPEGGYRL
                     KNPERGDRSDELLLVLTFSGGGTRAAALAYGVLEQLAQTEITLGGRKRTLLDEVDAIS
                     AVSGGSFTAAYYGLFGWKIFDDFETRFLKKNVQKDLILETIDLGNQFRLFSSTFGRSD
                     LAAEYYDKNIFEGKTFGDIMGRGGPLILINATDLVLGSHFTFTQDMFDLICSDVSRYP
                     VSRAVAASSAVPGLLTPITLYNYAGTCNYEPAEWMKKALEDPAASRRRTQLAQNLYTY
                     LDSKKRPFIHLIDGGPSDNLGLRGAIDRMNPMGDIWPSLQFMHWENTRKVVFILVNAE
                     KETKTESDQLETTLTSGEVLKYITNIPITRFNFETIELFKENARKWVEDIRSQRCGAR
                     KSEKPAEPSAGILNPCADIEFYLIEVDFDALPDKAERAYYKDLPTSFHLSYEAVDLLR
                     AAGRRVLIHSPEFQKLLRDLK"
     gene            5049..5774
                     /locus_tag="VEL68_03935"
     CDS             5049..5774
                     /locus_tag="VEL68_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXZ35152.1"
                     /translation="MFPGFVFLCLAFLHFSSPETLGQSPESPKFPPGRPEVDGGWNLP
                     ALDTAKNAGYLKELEKQVILELNKVRSDPPRYAKAFLFSQRGYYEGRRLTYPGETPIL
                     TQEGVKAFDEGYQFLLKARSASPLIPRVGLTQAARDHTLDQGKTGQTGHTGGDRSDVS
                     ERANRHGKWGGKVGENIQYGHASAARIVSSLLIDDGVPSRGHRKIIMEPLFRYIGVAF
                     GPHPKFRNMCVLVFAAEYSDSGR"
     gene            6112..>6534
                     /locus_tag="VEL68_03940"
     CDS             6112..>6534
                     /locus_tag="VEL68_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXZ35153.1"
                     /translation="MGDKIPTREEALKLLHQYFETDSLRKHAYAVEGVMRYIARKRGE
                     DVEKWGTIGLIHDLDYEKFPDQHCKKTREILEGEGWAEEYIRAAVSHGWGICSNEEPQ
                     TILEKTLYAIDELTGLVVTTALVRPSKSVMDVAVKSVKG"
BASE COUNT         1473 a         1847 c         1794 g         1420 t
ORIGIN      
        1 aaccatttga ttatgctgcc gcttgcggcc atgcacccgt taatcaggta tttaccgggg
       61 atgacgtggt aatcgagaaa gagccgaggg tccaccacca gtcggtccag gccatagagg
      121 atgtccccgg ccccgcccag ctttaccagc aggtcgccgg gagacttgac tcctgcggaa
      181 aaggccgagg cgatatggtc cgcgctgccg gtgacgaccg gcgttccctc agccaacccg
      241 gtgtggaggg cagctttccc atttaatcct ccaacaactt cgctcgacca ggaaactttc
      301 cccagccaat cgtggcttat cgtgctcagc ttcaggaggg attcatccca atcctcgcgg
      361 tgaaggtcaa agagaccgct ttccagggcc cagttccttt cgcaagagaa ggaccccgta
      421 agcctgtaga cgatatattc ataggagccc atcaggtgag ctgcctggtc catggcatcc
      481 ggttcgtggc gtcgcagcca gagcagcttg ggcccgatgc tttgctgggt gatggcgctt
      541 ccggtccttt gaagaacatt ttcctcatcc actatctgcc ggaattcgat gatctcttca
      601 aaagatcggg catcgttctg ctgtatcgac ggccgaagga cttttccaaa ccgatcgagc
      661 aggatgaggg tggggaccat tccgctcacc cctaccgcgc ccacatcttt tcttgctatg
      721 cccgcctttt ccagacaaat tcgggtaacc tctgccacgt tctgccacca ttgctccggg
      781 tcctcctcgg cccagccggc cttgcgcgaa atcagctgag ccggagcctc cgccttcgcc
      841 acaattccct gggagggatg aagcaaaatc ccctttgttg ccgtagttcc gatgtctatc
      901 cctagaagat atttttccat catcgcatca ggagatgact ttgcctctgc tctccgccct
      961 ctacgctctg cctccttttc tcaccgcgct caccgcttcc ataaacgctc tcacccgggc
     1021 cgggtccact ggattccaag tgtatccttt ctcttttaag ctggacccca cgatgacccc
     1081 gtcggccacg gtaaggaatt tctggatgtt ttccgcccgc gcacccgtgt tgagaagaac
     1141 gggtatggtc ggccccaccg cctcctttgc ctctttcagc caggaaaggt ccggttcgga
     1201 gccggccatg gaaccggaaa tgagaaccac atcaagaagg gaggagacca ccgcgctctt
     1261 ggctctttgc cctacgctcc gattccccag gggagaagcg aattcgggag tgatgttgcc
     1321 aaaaaccttc acgttctccg catcgatctg tttccgatag cggaagatgg ctgccgggtt
     1381 cggggcccag atgcccatgt cactctcgta aacaccggtt atcacctccc ggatgaaggc
     1441 ggctccggtg gccttggcca cggccagggg cgcccgggca tcccagagaa aatcgactcc
     1501 gaagggaatg tccctcggtt tgagttcggc aatgacccgg gccatgacgg caaccgcttc
     1561 caggtcggct ttgaaagaat agggacggtc gtcttcgttg cagaaaagga tcccgtccac
     1621 ccctccattc aggaggtgga tcacatcctg ccgcatccgg tccaggatgc cttccacccc
     1681 gcgcgaggca tcatagcgcg gagttccggg aagggcgggg aaatgggcca tggcaatgac
     1741 cggctttggc accccgaaca tctcctgcag gaggtcggac atgggaagcc cctttctgat
     1801 ccgtcatgat caagcttctg ggtaatttct ttttccattt tctaatgaat ttttatatat
     1861 aattattcca ggcttgtaaa cataaaaagg aaaaagcggt gccgataaga gaggttctct
     1921 aacaacccct caggcgagca tccttcgagg gctgaaaaat gggagaagac gatcttcggg
     1981 tgaccaagcc ggaggatttc agagactact gggacaaggt tcggcgaatc gtttatgata
     2041 ccgacccgga ggtctcggtc gagaagtggg agcaggagga tgtgaatttt gagggcgaat
     2101 acatcctcga cgggccggtc caaaaacagg cgaaacagcc cgagagcctg aaccccttcg
     2161 acttccgctg ggacagtcat gtcatcctcg ccggtctgac ggtaaaaaag gtcttgttcc
     2221 ggtcctacga cgggcaggag gtgggggggc tccttcaggt tccccgcttc tccgggatgg
     2281 accgcttccc gatgctcgtt cattatgccg gatacggggg agagattcta atcgattcag
     2341 attttgtgac cgccgggtac gcggtattca atttttccca ccggggcatg ctcatgggaa
     2401 gcaagggatt cgaccggtac catcccaccc cactcctggt gcagcacgtg gcagacaagg
     2461 atcgctacat atatcgatcg atcgtcatcg attgtctgct ggcgatcaag gtcatggcga
     2521 agacggagaa ggtggacccc tgccgattgg gtgtcatggg aacaagccag ggaggagcct
     2581 tagcgatcat cgctgcctcc ttgaatcccc aggtcaaagc cgcggcctgc gatcttcctt
     2641 ggttgaccga ttacgactac atcttgaacc atgacgtggg aggcctctac aacgaattga
     2701 aagaattttt acggaggaat ccggaaaagc ggaaggaggt cgcccgaacc ctcggatact
     2761 tcgacaccct gtccttcgcc gacggggtaa aaggcccgat tttcatgtcc ctgggggaaa
     2821 gcgaccccat cgctcccgcg gtctcggtga gaaaactctt ccagaacatc cctggaatca
     2881 agactctctt ggagaccccc ggaatgggac atgagcgcag cacgctgttc cgttatttgg
     2941 cccagaagtg gtttgatttc tttttataaa ttccccaaat cctatttttt tgaatgtccc
     3001 cggggaaaat tgattgccaa atcgccatca aaaccaggta tcctgtaatt tcatcccggt
     3061 aaacctccgg ctctgccggg ggacccatcg agtttgactg ttcctggaag cttggggcgg
     3121 ggcccggggg taaggggacc ttctgggcaa aaagcatcgg ccgggtgggc ccactcctta
     3181 gaggcttcga ccagcttctg caggatgcgg cgggcgcatc gggcgaccgg ccggtcgccc
     3241 ctacccgaag agaacacttt ccactgggga gtcggatggt tggcccgccc ggtccacgaa
     3301 ataggagctg cctcatgatt tttgcccaga aggtccttgc ccctgggcct ggcactcaat
     3361 cgagggccac cttatttgaa agacgagccc ggcggtcatg gggctgtgga caaaagaaga
     3421 tgaacggggg aattcaaagg ggccttgttc cggggaccga aatacgcaaa aagcccacaa
     3481 caacctggct ttatctttta aagaacgacc ggagacgtgc cgaatacatt ttgaaggcag
     3541 atacagctgg gtcatcgggg caccctggag agttctaagc caaccaaacc ttgactaaag
     3601 acggagggtt taaacgggct atgggaaagc ggaaaatcta ccggctgttt tggctcgggt
     3661 ttttcttggc tattctttca agtctgagct gtgcccacta ccccccaaac catgttctaa
     3721 cccaggtttc ccccgagggt gggtatcggc tgaagaaccc ggagaggggt gatcggtcag
     3781 acgaattgct gctggtccta actttttcgg gcggaggaac ccgggccgcc gccctggcgt
     3841 acggagtact ggagcaactc gcccagacag aaatcaccct gggaggccga aaaagaacct
     3901 tactggatga agtggacgcc atatcggccg tttccggggg cagttttacg gcggcttact
     3961 atgggctttt cggctggaag atctttgacg actttgaaac ccgcttcctt aaaaagaatg
     4021 tccagaagga cctcatcctg gaaaccatcg acttggggaa ccagttccgg cttttctcca
     4081 gcacattcgg acgcagtgat ttggcggccg aatattatga taagaatatt tttgaaggaa
     4141 agaccttcgg cgatattatg ggccgggggg gtcccctcat cctgatcaat gccacagacc
     4201 tggtcttggg gagccacttt accttcaccc aggatatgtt tgacctgatc tgttccgacg
     4261 tgagccgtta tcccgtatcc cgtgcagtgg ccgcttcttc ggccgtgccg gggctcttga
     4321 cccccataac cctctacaat tatgccggta cctgcaacta tgagcccgcc gaatggatga
     4381 aaaaagcatt ggaggacccc gccgcttccc gcaggcgaac ccagctggcc cagaatttgt
     4441 atacctatct cgattcgaaa aaacggccct tcattcatct gatcgatgga gggccttcgg
     4501 ataacctggg cctgaggggg gccattgacc ggatgaatcc gatgggggat atctggccct
     4561 ctctccaatt tatgcattgg gaaaataccc ggaaggtggt cttcatcctg gtgaatgcgg
     4621 agaaggaaac caaaaccgag tccgaccaat tggaaaccac tctgaccagc ggcgaggtct
     4681 taaagtacat aaccaacatt cccatcaccc gcttcaactt cgaaacgatt gaactattca
     4741 aggaaaatgc ccggaagtgg gtcgaagaca tccgttccca gaggtgcggg gccaggaaat
     4801 cggagaagcc ggctgaacca tccgcgggga tactcaatcc atgtgccgat attgaattct
     4861 acctcatcga agtcgatttc gatgcccttc cggataaagc tgaacgcgcc tattacaagg
     4921 atttgcccac ctccttccac ctgagctacg aagcggtgga cctattgcgg gcagccgggc
     4981 ggcgggtcct aatccattcc cccgaatttc agaagctcct tcgggaccta aagtgaggcg
     5041 ggccttgcat gtttcccggc ttcgtgtttc tctgtctcgc cttcctgcac ttttcttccc
     5101 ctgaaaccct gggccagtct ccggagagcc caaaattccc cccgggcagg ccagaagtgg
     5161 atgggggctg gaacctcccc gctttagaca ccgcgaaaaa cgccggttat ctgaaggagc
     5221 tggaaaagca ggtcatcctc gaattgaata aggttcggtc cgatcctccc cggtacgcca
     5281 aggccttcct cttcagtcag agaggttatt atgaagggcg aagactgacg taccctggag
     5341 aaaccccaat cctgacccaa gaaggggtca aagcttttga cgagggctac cagttcctgc
     5401 tgaaggcgag atcggcgagc cctttgattc cgagagtcgg attgacccag gcggcgcggg
     5461 atcatacgct ggaccagggg aaaaccggcc aaaccggaca tacggggggg gaccggagtg
     5521 atgtttccga gcgggcgaat cgtcacggga agtggggagg gaaagtcggc gaaaacatcc
     5581 aatatgggca tgcgtccgca gcccgaatcg tatcgtccct gctgatcgat gatggagttc
     5641 cttcccgggg acatcgaaaa atcatcatgg aacctctctt ccggtacatt ggagtcgcct
     5701 tcgggcctca cccgaaattt cgcaacatgt gcgtcttggt ctttgccgca gagtattcgg
     5761 attccgggcg atagcttccc cttgggtgct cagcgcctca ttcaggctag catgttggca
     5821 aaaaggaaat atccgggtcc caaggccggt aggggcggtt cgcgacctgc ccgtgctgct
     5881 tttggccaaa gggcacttat ccttcaggcg gtcgcggaga aaccttaatc ctttcatccc
     5941 accttgtaga cctgattcca aaggcattct tcgagcaccc actcattcat accttgctcg
     6001 ccacaaaggc caacaattcc gttgacagaa agggagaatt tctatacctt gaacccagtt
     6061 ctctttccac cgatacgaac atcgacttta aactccagga ggactttggt catgggagac
     6121 aagattccca cccgcgaaga ggccttaaag cttcttcacc agtattttga gaccgacagt
     6181 ctgcgcaagc atgcctatgc ggtcgaaggg gtcatgcgct atatcgcccg caagcgcggc
     6241 gaggatgtag agaaatgggg gaccattggc ttgatccacg acctcgatta cgaaaaattc
     6301 ccagaccagc actgcaaaaa gacccgggag atcctggaag gagaaggctg ggcggaagaa
     6361 tacatccgcg ccgccgtctc ccacggctgg gggatctgca gcaacgagga gccccaaacc
     6421 atcctggaaa aaaccttgta cgccatcgac gagctgaccg gcctggtcgt caccacggcg
     6481 ttggtgcgcc cttctaagag cgtaatggat gtagcggtga aatccgtaaa gggc
//
LOCUS       DASPRR010000111         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2287863, whole genome shotgun sequence.
ACCESSION   DASPRR010000111 DASPRR010000000
VERSION     DASPRR010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287863"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /gene="cdhC"
                     /locus_tag="VEL68_03945"
     CDS             <1..1063
                     /gene="cdhC"
                     /locus_tag="VEL68_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_function: GO:0043884 - CO-methylating acetyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HXZ35154.1"
                     /translation="LKPPAQLPLAIVAEVAGRQMQEDFEPILERQIHHLINYAQGVMH
                     IGQRDIAWIRVGKGAVEKGFTLRHLGDIIHAKYHQDFGTIFDKVQVKVYTDLDKVKQI
                     MEQARATYRKRDARIEGMTDETEETFYSCTLCQSFAPTHVCVITPERTGLCGAYNWLD
                     GKASNEINPTGPNQPIIKGEAIDPLMGQWKGVNEFVFKASRQKLEKYNAYSIMVDPMT
                     SCGCFECIAAVLPMCNGIMTVNRDFTGMTPCGMKFTTLAGSVGGGSVTPGFVGHSKHY
                     ICSKKFIMAEGGIKRLVWMPKVLKEEIMDRLKKRSEEIGIPNFPDMIADETIGTTEDE
                     ILPFLTEKGHPALTLPSLF"
     gene            1156..2508
                     /gene="acsC"
                     /locus_tag="VEL68_03950"
     CDS             1156..2508
                     /gene="acsC"
                     /locus_tag="VEL68_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HXZ35155.1"
                     /translation="MALTGIEIFKKLPKTNCGDCGVPTCLAFAMKLAAGQAELAACPH
                     VSEQAKAELSEASAPPIRPLSIGTGDQALKLGGETVLFRHEKRFVSPTGIAVLVSDKM
                     GDSEVDSRLKKFNELKYERVGLKLQADLVAVKGESGDPARFEALVKKVKERLDGKGVV
                     LMSEDPNVLAAGAKAVADRKPLLYAATKANADAMAKLAKEVSAPLAIKGGTPDELAEL
                     ATKLTAAGVKDLVLDSGARTPRKALEDQIVIRRAALLKKFRPLGFPTITFPCEMTQDP
                     MMETLLASMLVAKYAGIVVLSDLDGHSLFPLLVERMNIFTDPQRPMATSEGIYEIGGP
                     NENSPVLVTSNFSLTYFIVSGEIEGSRVPSWLLVKDTEGLSVMTAWAAGKFGADVIAP
                     FVKKCGIAGKVKHHKLIIPGYASVISGELEEELPDWKILIGPREAGHIPAFLKMWKPE
                     "
     gene            2613..3494
                     /locus_tag="VEL68_03955"
     CDS             2613..3494
                     /locus_tag="VEL68_03955"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXZ35156.1"
                     /translation="MVVCVGENINVMSKTIGPAMKEHKADPIVEMAKHQEKVGIDYLD
                     LNIGPSRKAGQEIMDWIVKLVQGVVNKPMSLDTTNPDAMEAGLKVHKGKALINSVSLQ
                     PERLERVLPMAKTYNAHVIGLLWGKEGMPRDANERASMAVDFIYQANNLGIPTEDIWI
                     DPIVSPVSVEINQVLSCLEFLSMLKDIAPEAKSIVGLSNISNGTPTHLRPVLNRTYLV
                     MLMKYGLYSAIVDAYDEEILRICRGGLPEMVSLVHRVMDGDEPDPKRLTPKELEYFKS
                     AKVLLGKTLYSHSWLEL"
     gene            3511..4167
                     /locus_tag="VEL68_03960"
     CDS             3511..4167
                     /locus_tag="VEL68_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HXZ35157.1"
                     /translation="MNRPYESFMLPKQENLPLARRMAVEGILKLDPAERAARGGGRFE
                     PGMDGEGRILLPYLGRDLSLSVSRGAIELRNGGDPLSLREEILVLHYLEKASGNPATG
                     QWVSFSEIPGGTFYHPVFLLRGKAPLVRHFGKEAQRLLILAIDEMGGRPLALGDVGVR
                     IPAFPRVDVGLVLWKGDEEFPPEGNILFDSSISGYLPVEDIVILAETVVWKLIKAGHR
                     "
     gene            4267..>4714
                     /locus_tag="VEL68_03965"
     CDS             4267..>4714
                     /locus_tag="VEL68_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ35158.1"
                     /translation="MKLAVTGKGGVGKTTVVAGLARVLADRGRKVLVIDADPASNLAL
                     ALGFPKDKRLSPIAEMKELIEERTEAKPGSMGGFFKLNPRVDDLPDKYSLEKDGIRLM
                     VMGTVQKGGGGCVCPENVMVRTLVAHLLLGRNEVVILDMEAGVEHLG"
BASE COUNT         1061 a         1329 c         1430 g          894 t
ORIGIN      
        1 cctgaaaccc ccggcccagc ttcccctggc tatcgtggcc gaggtggccg gccggcaaat
       61 gcaggaggac ttcgaaccga tcctggagcg gcagatccac cacctgatca actacgccca
      121 gggggtcatg cacatcgggc agagggacat cgcctggatc cgggtcggga agggcgcggt
      181 ggagaagggc ttcaccctcc gtcatctggg ggacatcatc cacgccaagt accatcagga
      241 cttcgggacc atcttcgaca aggtgcaggt gaaggtctac accgacctag acaaggtcaa
      301 gcagattatg gagcaggccc gcgctaccta ccgcaagcgg gacgcccgca tcgagggcat
      361 gaccgatgaa accgaagaaa ccttctattc ctgcaccctc tgccagtctt tcgctcccac
      421 tcatgtgtgc gtcatcaccc cggagcggac cggcctctgc ggggcctaca actggctgga
      481 cgggaaggct tccaacgaga tcaaccccac tgggcccaac cagcccatca tcaagggaga
      541 ggccattgac cccctcatgg ggcaatggaa aggggtcaac gagttcgtct tcaaggcctc
      601 acggcagaag ctggagaagt acaacgccta cagcatcatg gtcgacccca tgacttcctg
      661 cggctgcttc gagtgcatcg ccgccgtcct tcccatgtgt aacgggatca tgaccgtgaa
      721 ccgcgatttt acgggaatga ccccctgcgg gatgaagttc accaccctgg cgggatcggt
      781 gggcggcggc tcggtgaccc ccggtttcgt gggccacagc aaacactaca tctgcagcaa
      841 gaaattcatc atggccgaag ggggcatcaa acgcttggtc tggatgccca aggtgctaaa
      901 ggaagagatc atggaccggc tgaaaaagcg ctccgaggaa atcggcattc ccaacttccc
      961 ggacatgatc gccgacgaaa ccatcggcac cacggaggac gaaatcctgc ccttcctgac
     1021 cgagaaaggg cacccggcgc tgaccctgcc ttccttattc taggggcgat gtttggttga
     1081 aaatcccgta ggggcgattc atgtcggaga ctcgactcca cgagaatcgc ccctacgttg
     1141 aaaaggagca caaagatggc tctaaccggt attgagattt tcaaaaagct tcccaagacc
     1201 aactgcggag actgtggggt tcccacctgc ctggcctttg ccatgaagct ggccgccggg
     1261 caggccgaat tggcagcctg tccccatgtg tcggagcagg ccaaggccga gctgtccgaa
     1321 gcctcggccc ccccgatccg tcccctttcc atcggcacgg gagatcaggc cttgaagctg
     1381 ggcggggaga ccgtcctttt ccggcatgaa aagcgatttg tcagccccac ggggatcgcc
     1441 gtactggtct ccgacaagat gggcgacagc gaggtcgatt cccgcctgaa gaaattcaat
     1501 gaactgaagt atgagcgagt cggtctgaag cttcaagcgg acctggtggc agtcaaggga
     1561 gaatcgggag accccgccag gttcgaggcc ctggtgaaga aggtaaaaga aaggctggac
     1621 ggcaaagggg tcgtgctcat gagcgaagac cccaacgttc ttgccgctgg ggccaaggcc
     1681 gtagccgatc ggaaaccgct gctctacgcc gcaaccaagg ccaacgcgga cgctatggcc
     1741 aaactggcca aggaagtctc cgcgcccctg gcgatcaagg ggggaacccc ggacgaactg
     1801 gcagagctgg ccaccaaact gaccgccgcc ggggtgaagg acctggtgct ggattcgggg
     1861 gcccggaccc cccggaaggc gctggaagac cagatcgtca tccggcgggc ggccctgctg
     1921 aagaaattcc gccccttggg atttcccacc atcaccttcc cctgtgagat gacccaggac
     1981 cccatgatgg agactctcct agcttccatg ctggtggcca agtacgcggg aatcgtggtc
     2041 ctctccgacc tggacggcca cagcctcttc cccctcctgg tggaacggat gaacatcttt
     2101 accgatccgc agcggcccat ggctacctcg gagggaatct acgaaattgg cgggccgaat
     2161 gagaattctc cggtgctcgt cacctccaat ttctccctaa cctactttat cgtctcggga
     2221 gagattgaag ggagccgggt cccctcctgg cttctggtga aagacaccga agggttgtcg
     2281 gtcatgaccg cctgggccgc gggaaaattc ggggccgatg tgatcgcccc cttcgtgaaa
     2341 aagtgcggga tcgccggcaa ggtgaagcat cacaagctaa tcatccccgg ctatgcatcg
     2401 gtaatcagcg gagagctgga ggaggaactg cccgattgga agatcctgat tgggcctcgg
     2461 gaagccggcc atatcccggc tttcctaaaa atgtggaaac cggaataaaa aaagtgtcag
     2521 tgtcagtggt gggtgtcagt gaaaacatca aaattcaaga gcttttgact gacaccaatc
     2581 actgacactg tcttttcaaa aggaggggag agatggttgt ctgcgtcggc gagaacatca
     2641 acgtcatgtc caaaaccatc ggtccggcca tgaaggaaca caaggccgac cccattgtgg
     2701 aaatggccaa acaccaggag aaggtgggga tcgactacct ggacttgaac atcgggccct
     2761 cccgcaaagc cgggcaggaa atcatggact ggatcgtgaa gctggttcag ggggtggtga
     2821 acaagcccat gtccctggat accacgaatc ctgacgccat ggaagcggga ctgaaagttc
     2881 acaaaggaaa ggcgctgatc aattccgtat cgctccagcc ggagcgcctg gaaagagtgc
     2941 tgcccatggc caagacctac aacgcccatg tcatcggcct tctttggggc aaggagggaa
     3001 tgccccggga tgcgaacgag cgggcctcca tggccgtgga tttcatctat caggccaaca
     3061 acctggggat tcccacggaa gacatctgga tcgaccccat cgttagcccg gtgagcgtgg
     3121 agatcaacca ggtcctttcc tgtctggagt tcttgtccat gctgaaggac atcgctcccg
     3181 aggccaaatc catcgtgggg ttgtccaaca tctccaacgg gaccccgacc catctccggc
     3241 cggtcctgaa ccggacctac ctggtgatgc tgatgaaata cggcctttat tcggccatcg
     3301 tggacgccta tgacgaggag attctccgca tctgccgcgg cggcctcccc gagatggtta
     3361 gtctggtcca tcgggtgatg gatggggatg agcccgatcc caagcgcctg acgccgaagg
     3421 agttggagta tttcaagagc gcgaaggttc ttctgggaaa aaccctatac tcccattcgt
     3481 ggctggaatt ataaactgta ggggcgattc atgaatcgcc cctacgaaag ttttatgctg
     3541 cccaaacaag aaaacctccc cctggcccgc cggatggcgg tggaggggat tctgaaacta
     3601 gacccggcgg aaagagcagc ccgtggcggg gggcggttcg aacccggcat ggacggggaa
     3661 ggaaggatcc ttctgcccta tctggggcgg gacctcagtc tttccgtttc gcggggggca
     3721 atagagcttc ggaatggggg agaccccctc tccctgcggg aagaaatcct ggtcctccac
     3781 tacctggaaa aggcatcggg gaatcccgcg accgggcaat gggtttcctt ctccgaaatc
     3841 cccgggggga ctttttatca tcctgttttc ctgctgcggg gcaaggctcc cctggtcaga
     3901 catttcggga aggaagcgca aaggcttttg attttggcta tcgatgaaat ggggggaaga
     3961 cctttagccc taggggatgt aggagtaaga attccggctt ttcctcgggt ggatgttggg
     4021 ttggttctgt ggaagggaga cgaggaattt cctcccgagg gaaatatcct cttcgattct
     4081 tctatcagcg gctatctgcc cgtggaagac atcgtcatcc tggcggagac ggtcgtttgg
     4141 aagttgataa aagcaggtca taggtaatag atcataggtt atgggttata ggttataggt
     4201 gataggcctg aaaagcatac cccaagacct attacccatg acctctaacc cattacccgt
     4261 attattatga aactagcggt gaccggaaaa ggtggggtgg ggaagaccac ggtggtggcg
     4321 gggctggccc gggtgctggc agaccggggg cggaaggtcc tggtgatcga cgccgacccg
     4381 gcgagcaacc tggccctggc cctgggcttt cccaaggata agcgcctctc acccattgcc
     4441 gagatgaaag agctgatcga ggaacggacc gaggccaagc ccggttccat gggcgggttc
     4501 ttcaaactca acccccgggt ggatgatctt cccgacaagt acagcctgga aaaggatgga
     4561 atccgtctga tggtcatggg gaccgtccag aagggcgggg ggggctgtgt ttgcccggaa
     4621 aacgtcatgg tccggactct ggtggcccac cttcttttgg gtcggaacga ggtggtcatc
     4681 ctggacatgg aggccggggt cgaacacctg gggc
//
LOCUS       DASPRR010000112        12683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1804524, whole genome shotgun sequence.
ACCESSION   DASPRR010000112 DASPRR010000000
VERSION     DASPRR010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12683)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12683
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804524"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VEL68_03970"
     CDS             complement(<1..222)
                     /locus_tag="VEL68_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014121635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ35159.1"
                     /translation="MKAMVKEKPGAGAAIKDLDIPKPGPRDVLIRVKVGAICGSDVHI
                     FEWDSWAQSVVPKLPQILGHEFAGEVVEVG"
     gene            359..1507
                     /locus_tag="VEL68_03975"
     CDS             359..1507
                     /locus_tag="VEL68_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ35160.1"
                     /translation="MADRVGIVGIYQTKYEPRKVFDSYPELVFEASSRVLEGTGLTIG
                     NMDQIVTNSQDFWDGRTISNRTVSEACGSVLRSESKVAMDGSFAVFYAAMRILSGCYD
                     SCLLVSHCKMSEGDPHLINNAMFDPLYQRVLGLDEVSTCALQARRYMNKHKISEEQCA
                     RVSVKNLRNGKLNPHVHRAMEVNINQVLRSAKLADPIKELDAYPVTDGACAMILASEE
                     KAKKWCPNPVWLAGMASRLDSFYLGDRDLAETASLTLAAQKAYQMAGISNPAKEIDLV
                     ELSDFYSYQELLWLEGMGFCRRGEAGKMVEKGITGLEGELPVNPSGGILSGNPVTVAG
                     AIRVAEAALQLMGQAGKHQVEGAKTALAQGHCGPCGQGHCVIVLEKGV"
     gene            1513..2679
                     /locus_tag="VEL68_03980"
     CDS             1513..2679
                     /locus_tag="VEL68_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ35161.1"
                     /translation="MARRSKRNVAIVATGQTDHRSKRPDVNIVEMINEAVRRCLADAR
                     MTMDQIDAIVIGNMEHFEGIFFTEMWSVDAAGAFLKHGMKITTGGTTGSSLAQAGYYH
                     VASGLFDTVMTIGWEKQSEGETTAGLIFQADPLWERATMSGAIGSFAGSATAYMHKYG
                     ITEEQAAKVAVKNRRNAIQNPHAHLKMDLTVEQVLNSKMLAYPIKILDMCPTSDGACA
                     MIFASEKKAKEVCPTPAWVIAAVTRHDQPFGGDLEMVMNLRTLRSATEEAYKIAGIKD
                     PLRDFDVAELYEPCTFAELSWYEATAFCGPGEGGKLMDSGATQMDGELPVNPSGGVLS
                     TNCIGATAMIRVAEAAIQVMGKGGKRQVPNVNRAIATGFGGSWWSDVMILSRTP"
     gene            2879..3349
                     /locus_tag="VEL68_03985"
     CDS             2879..3349
                     /locus_tag="VEL68_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ35162.1"
                     /translation="MNDKGGSILVAEFDAKFPYDYSAGRYGSYFFKKLKDEKRIMGIR
                     CPKCDRVFVPPRPACGYCFVKNTDWVELGDEGTLWGYTVVQFPFLDPLTGAERPIPYG
                     YGFIELKGAATRLQHFVTASDLNKLKIGMRMKAVFRDERKGDLTDILYFKAIEE"
     gene            3365..4036
                     /locus_tag="VEL68_03990"
     CDS             3365..4036
                     /locus_tag="VEL68_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ35163.1"
                     /translation="MEKWLEGLEPVVVHQDLRVPYRYSMGAVPSKFFIEIRDHQKIMG
                     IRCPSCDVVFIPPRSTCGRCFSQLHEWVEVGNQGTLETYTQVRYSTPVQPAVAPFYYG
                     IIKPDGADTGLAHMVGDLKGKEPRIGMRVQAVFKEERKGNMLDILYFKPVKEEKKAGD
                     RGQKGRKGEKAKRRKVVKGKEERKGKKISKKKTANVKTTKTVKRAKSVRKRATVPRRK
                     AKREK"
     gene            complement(4110..5561)
                     /locus_tag="VEL68_03995"
     CDS             complement(4110..5561)
                     /locus_tag="VEL68_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXZ35164.1"
                     /translation="MGRTSIDLKRALTADAFLRASGAPLVHGNSLRILRDGEENYPEW
                     LKAIEAAQKTIHLEMYIIHNDQTGRRFRDLITEKARQRVKVRVLYDWVGSHGPLAYRM
                     WKPAREAGAEVRAANPPSMDSLLGWASRDHRKLLTIDGSLAFISGLCMGDAWAGDPSR
                     GIPPWRDTGVEIRGPAVADAEVSFAEAWKTVGGSIPVAQISRRENLPRAGNVSLRVVA
                     ASPDTASLYRLDLMVAASASKTLWLSDAYFIGTTAYLQALRSAALDGVDVRILVPHGS
                     DIQWIANVSRTLYRSLLEAGVRVFEWNGPMMHAKTAVADGRWARVGSTNLNIASWIGN
                     WELDVVVENENIGRQFSRMFLEDLANSTEIVITRRNKVRLTQPLPVAGQTRTSSGSGK
                     HVLTGVFRVSSAINAAVTGHRVLSRTESTSLLSICVAVLVLASVALFLPKVIAYPVGV
                     ILAWMGILLLGKGLRIRFSKEARKKIREKKKAP"
     gene            complement(5637..6374)
                     /locus_tag="VEL68_04000"
     CDS             complement(5637..6374)
                     /locus_tag="VEL68_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXZ35165.1"
                     /translation="MSQSQEPLRLRIVTYNVHKCRGMDGRTHPERIASVLGRLNPDIV
                     ALQEVIGAGPRGFGQEERISQRLGMASVLAPARTLRGHLYGNALLSRIPIKHQSAFDL
                     SEDGLEPRLLQRVDLSFQGHRIHLYNVHLGTSQKERERQARKLIYFLSDPSVRGPKIL
                     LGDFNEWRKGPASRLLAETFQRLALLPFLKWRRTYPGFLPIFHIDHIYYQGQVEVLKV
                     EVPRTLSALIASDHMPILMELQISLKK"
     gene            complement(6539..6745)
                     /locus_tag="VEL68_04005"
     CDS             complement(6539..6745)
                     /locus_tag="VEL68_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXZ35166.1"
                     /translation="MIDDSTTPEEAIRLAMQREKAAFEFYTKAAKIAKYPGTREMFES
                     LAKEELRHARLLEEELNKDYYKEM"
     gene            complement(6742..7365)
                     /locus_tag="VEL68_04010"
     CDS             complement(6742..7365)
                     /locus_tag="VEL68_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HXZ35167.1"
                     /translation="MQVKNQMSKKVVTIPPDTSILRAIEIMHEKSIRHLPVVEEGDLV
                     GLVTEGDLRQASLLSLVDKVSIEDVMIKKPVTVSPEDSIEEAAKLIYRHKIGGLPVVA
                     GKKLVGILTIVDILAAFIQLLGILKSSSRIDVVLGGRAHAFEEVSAIFRKHKTEIISL
                     GMSNHKDRKKRVYYFRVRKYPVIPLVTDLQKKGYKVLSVVEPFRRPG"
     gene            7545..8852
                     /locus_tag="VEL68_04015"
     CDS             7545..8852
                     /locus_tag="VEL68_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXZ35168.1"
                     /translation="MADPFWEREIETLKPKELRRLQLRRLKKTLRQAATSPFYSGLFK
                     KHQVSAEKVESLEDLKEIPFTTKQDLRDQFPYGLLTVPREKIVRLHSSSGTTGRATAV
                     FHTQKDLDGWANLVARCMYMAGVRKQDVFQNMSGYGLFTGGLGFQYGAERLGALTIPS
                     GSGNSKRQIHLMQDFGTTVVHIIPSYALHLWKVFGEMGLEPKRDTQLRILLIGAEPHS
                     EATRQRIEEMYGVDAFNSYGLSEMNGPGVAFECPQKKGMHFWEDAFLVEVIDPKTGEV
                     LPDGVEGELVYTTLNREAMPMIRYRSKDLATIFPELCPCGRTHRRMSRIKGRSDDMFI
                     LKGVNIFPLQIDTTLMNIPEVGTNYLIVLERENFEDRMIVRVEVRPQFFKGDLWQLEK
                     LRKQITEALKSELLVTPKVELVEPDSLPKSEGKAVRVMDKREM"
     gene            8970..9398
                     /locus_tag="VEL68_04020"
     CDS             8970..9398
                     /locus_tag="VEL68_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXZ35169.1"
                     /translation="MAHQISVFVENKPGRIERVTEFLMKAEVNIRAITVMGTFEYGVI
                     KLLVDKPDIALEALRSQGISAHKREIIAILMDDRPGGLHRIAKVFGQKKINIDDAYGF
                     VIEDKKRAVLVVDVGKIQEAEKVLKEEGLTILSDREIYSL"
     gene            complement(9594..9872)
                     /locus_tag="VEL68_04025"
     CDS             complement(9594..9872)
                     /locus_tag="VEL68_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HXZ35170.1"
                     /translation="MAEVKGIGYRVVGTIKSVKGHCNAGHKAGQQLELSGHNTAGMCG
                     FFYHDAFPYILMLQFGGGFPMEWGGPDKVELECMDRTNAVTIELKRAR"
     gene            complement(10020..11336)
                     /locus_tag="VEL68_04030"
     CDS             complement(10020..11336)
                     /locus_tag="VEL68_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ35171.1"
                     /translation="MPTKEDLKKKVIGSVEKRAKEITEIAETILRNPELGYKEVKTAA
                     LVEDKFRSMGLSYKKGLALTGVVAHIAGKSKKLQVAVMGELDSIRIPVHPFADPLTGA
                     AHGCGHNAQIAGMLGAGMGLLDSGVMKELDGDVSLMAVPAEELVELEYRNKLREEGKI
                     AFLTGKQEFIRLGLFDAIDVSLMFHTELAEPPQKVAVGVTMNGCLAKFICYKGKESHA
                     GGAPHLGVNALNAALLGLMGIHAQRETFKDEDSIRVHPILTKGGDLVNVVPGDVRIET
                     FVRGRTAEAFQDAHRKVNRALKAGAMAVGAEVEIRNLPGYLPMQNSERLDGLFAANMA
                     YLLGPEAIGRSPHLTGSSDMGDITHLMPGLHPMIKAGSAKVHTESFCIEDTRLACIET
                     AKGLAMTVIDLLWDGAREGLAIKSAYKPKYNKEQFLKFWEELCKEA"
     gene            complement(11396..>12683)
                     /gene="gcvPB"
                     /locus_tag="VEL68_04035"
     CDS             complement(11396..>12683)
                     /gene="gcvPB"
                     /locus_tag="VEL68_04035"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXZ35172.1"
                     /translation="VRHFTRLSQWNYGVDSGFYPLGSCTMKYNPKVNEDIARLPGFAG
                     AHPYQPEEVSQGELQLIYELEHYLGEVSGMDRVTLQPSAGAQGELTGMLMIRAYLMDR
                     GNPRKKVLVPDTAHGTNPASSSLCGYQVVQVKSDERGRLSPRAIEEKMDEDVAALMVT
                     NPNTLGLFEDHICEVAEIVHKRGGLIYCDGANLNALLGLTRVGDMGVDVLHFNLHKTF
                     STPHGGGGPGAGPVGVKGHLADYLPIPVVAKEGERFRFEYNRPKSIGKVRSFYGNFGV
                     LVRAYSYILSMGPEGLKKASLLALLNANYIRARLKGTYSLPYDFPCMHECVFNDKVQN
                     KFGVTALDIAKRLMDYGFHPPTIYFPLVVHGALMIEPTESEAKETLDQFIESMIQIAK
                     EAETDPEVVRSAPHRTKVFRLDEVLANRKPKLRWKP"
BASE COUNT         2922 a         3401 c         3574 g         2786 t
ORIGIN      
        1 cccgacttcc accacctctc cagcgaattc atgccccaga atctgcggaa gtttggggac
       61 caccgattgg gcccacgaat cccactcaaa aatgtgaacg tcggagccgc agatcgctcc
      121 caccttcacc cggattaaaa catcccgggg acccggctta ggaatatcca aatcctttat
      181 tgccgccccc gcccccggtt tttctttgac cattgctttc atttcttcct ccttttagcc
      241 agacctaaag cgcccgcaca aaatccattc tgacatgaaa tttctcttgc gacaactcgg
      301 tcattttact acaatgcggc gcagatggcg attcgactat ggaacagggg gtaaggcgat
      361 ggctgatcgg gtcggaattg tgggaatcta tcagacgaag tatgaaccgc gcaaggtctt
      421 tgacagctat ccggaattgg tctttgaagc ctccagcagg gtgctggagg gaacgggatt
      481 gaccattggg aatatggacc agatcgtcac caattctcaa gatttttggg acgggcggac
      541 gatctccaac cgaacggtga gcgaagcctg cgggtccgtt ctgcggtccg agtcgaaagt
      601 ggctatggac gggagtttcg cggtcttcta tgccgccatg cgcattttga gcggctgcta
      661 tgactcctgt ctccttgtat cgcactgcaa gatgtccgag ggggaccctc atttgatcaa
      721 caacgccatg ttcgaccctc tttaccagcg ggtcctgggg cttgacgagg tttccacctg
      781 tgctcttcag gcgagaaggt acatgaacaa acacaagata agtgaagaac agtgcgcccg
      841 ggtgtcggtg aaaaacctga ggaatggaaa gctcaatccc catgtccatc gggccatgga
      901 ggtcaatatc aatcaagtct tgcgttccgc caagctggcc gacccgatca aggaactgga
      961 tgcataccca gttactgacg gggcctgcgc catgatcctg gccagcgaag agaaggccaa
     1021 aaaatggtgt ccaaaccccg tatggctcgc cggaatggct agccgcttgg attccttcta
     1081 tctgggagac cgggacctgg ccgaaacagc ctccctcacc ctagccgccc aaaaagcgta
     1141 tcaaatggcc gggatttcca atcccgccaa agagatcgac ctggtggaac tctccgactt
     1201 ttactcctac caggagctcc tatggctgga ggggatgggt ttctgccgcc gaggagaagc
     1261 ggggaaaatg gtggaaaagg gaatcacggg actggaaggg gaacttccgg tgaatccttc
     1321 cgggggcatc ctttcaggaa atccggtcac cgtggctggt gcgatccgcg tggccgaagc
     1381 cgcgctgcag ctcatgggcc aggcgggaaa gcaccaggtg gagggggcca agacagccct
     1441 ggcccagggg cactgcgggc cctgcgggca aggccattgt gtaatcgttc tggaaaaggg
     1501 ggtttaacgg ccatggccag gagatcaaaa agaaacgtgg cgattgtggc gaccggacag
     1561 accgatcatc gatccaagcg cccggatgtg aatattgtgg aaatgatcaa cgaagccgtg
     1621 cgccggtgcc tggccgacgc ccgtatgacc atggaccaga tcgatgcgat cgttatcggc
     1681 aatatggaac attttgaagg gatttttttt accgagatgt ggtccgtgga cgcggccggt
     1741 gccttcctga agcacgggat gaagatcacc accggcggga cgaccggaag ctccttggcc
     1801 caggccggtt attaccatgt ggcctcggga ttgtttgaca cggtcatgac cattggctgg
     1861 gaaaagcagt ccgaggggga aaccacagcc ggattgattt tccaggccga tcccctttgg
     1921 gagcgggcca ctatgtccgg ggccattggg tcctttgccg gttcggccac cgcctacatg
     1981 cataaatatg gaatcaccga agaacaggcg gccaaggtgg cggtgaaaaa ccgcaggaat
     2041 gccattcaaa acccccatgc ccatctgaag atggacctca ccgtggaaca ggtcctcaat
     2101 tccaagatgc tggcctatcc cattaagatc ctggacatgt gccccacctc ggatggggcc
     2161 tgcgccatga ttttcgcctc ggagaagaaa gccaaggaag tctgtccgac cccggcctgg
     2221 gtcattgccg cggtcacccg gcacgaccaa cccttcggcg gagacctgga aatggtgatg
     2281 aacctgcgga ccctccgttc cgccaccgag gaggcgtaca agattgccgg gatcaaagac
     2341 cccttgcgag attttgacgt ggccgagctc tatgagccct gcacctttgc cgaactctcc
     2401 tggtacgagg ccacggcctt ctgcggcccc ggggagggag ggaaattgat ggattcgggg
     2461 gccactcaga tggacggaga gctgccggtc aatccttccg gaggagtgct ttcgaccaac
     2521 tgcatcgggg ccacggccat gatccgggtg gctgaggcgg ccatccaggt catgggcaaa
     2581 gggggaaaga ggcaggtccc caacgtcaac cgggccatcg ccaccggttt cggggggagc
     2641 tggtggtccg atgtgatgat tctttcaaga accccataaa tataaattcg aaatccgaat
     2701 atcgaaattc gaaacaaacc cgaataggca aaatacaaaa tttgaaaagg gctttggaaa
     2761 atttgggatt taaaatttgt ttcggatttc gtgcttcgaa tttcagagtt atcggagagg
     2821 ctgtccagac ttagaccaac ttagaatttt ggatcctctg ttaaaggagg caggagtcat
     2881 gaatgataag ggcggttcga ttctggtggc ggagttcgac gctaagttcc cctacgacta
     2941 ctccgccggg cggtatggca gctatttttt caagaaactg aaggatgaaa agaggatcat
     3001 ggggatccgc tgtcccaagt gcgaccgggt cttcgtgccg ccccgtccgg cgtgcgggta
     3061 ctgctttgtg aaaaacaccg actgggtcga gctaggggat gagggtacgc tctggggcta
     3121 caccgtcgtt cagttcccct tcctcgaccc tctcactggg gcggagcgcc ccatccctta
     3181 cgggtatggg tttatcgagc tgaaaggggc tgcgacccgg ctacagcact tcgtcaccgc
     3241 ttcggacctg aacaagctca agatcgggat gcggatgaag gccgtcttcc gggacgaacg
     3301 gaagggagac ctgacggata tcctctattt caaagcgata gaagaataag aaaagaggtt
     3361 gatcatggaa aaatggctcg aagggttaga acctgttgtg gtccaccagg atttaagggt
     3421 gccttaccgg tattccatgg gagcggtacc gagcaagttc tttatcgaaa tccgggatca
     3481 tcagaaaatc atgggaatcc ggtgcccctc atgtgatgtg gtgttcatcc ctccccgcag
     3541 cacatgcggg cgctgcttct cccaactcca tgaatgggtt gaagtcggca accaggggac
     3601 actggaaacc tatactcagg ttcgttattc aaccccggtt cagcccgccg tagccccttt
     3661 ctattatgga atcatcaaac cggacggtgc tgatactggc ctggcccata tggtcggtga
     3721 tttaaagggc aaagaacccc gcattggcat gcgcgttcag gcggtcttca aagaagaacg
     3781 taaggggaac atgctggata ttctctactt taagcccgtc aaggaagaaa agaaggcagg
     3841 agacaggggc caaaaagggc gaaaggggga aaaggccaaa cggcgaaaag tcgtgaaagg
     3901 caaagaagag agaaaaggaa aaaagatttc aaagaagaaa acagccaacg tcaagacaac
     3961 gaaaacggtg aagcgggcga aatccgtaag gaagagagcc acggtcccga ggcgcaaagc
     4021 caaaagggaa aaatagaacc ggccagccat gagggcatga tgcatcatgc ccctacttag
     4081 ggcgaatcgg atagaggcca gggagcaatt cagggggcct tcttcttttc ccggattttc
     4141 ttcctggcct ctttgctgaa gcgaatccga aggcctttgc caagcaacag aattcccatc
     4201 cacgctaaaa tcacgcccac agggtaggcg ataaccttgg gtaaaaagag ggcgacagaa
     4261 gccaggacca gcaccgcaac gcaaatgctc aagagggagg tcgattccgt ccggctcaat
     4321 acccggtggc cggtgactgc ggcattgatg gcgctgctca cacgaaaaac cccggttaat
     4381 acgtgcttcc cgctcccaga agaagttcgg gtttggcctg ctacgggcaa aggctgggtg
     4441 aggcggactt tgttccgccg agtgatgaca atctccgtcg agttggccag gtcttccagg
     4501 aacatccggc taaattgccg gccgatgttt tcgttttcca ccaccacatc caattcccaa
     4561 ttcccgatcc agctggcgat attgaggttg gtggagccga ctcgcgccca acgcccatcg
     4621 gccacggccg ttttggcgtg catcatgggc ccgttccatt caaagacccg cacgcccgct
     4681 tcaagcaggg accgatacag ggtgcgggag acattggcga tccattgaat atcgcttcca
     4741 tggggcacca agatgcgcac atccactccg tccagggccg cggagcgcaa agcctgaagg
     4801 taagcggtcg tcccgatgaa gtaagcgtcg gaaagccata gagtcttaga ggctgaggcg
     4861 gccaccatca aatccaagcg gtacagacta gcggtatccg gtgaagccgc gacaacgcgg
     4921 agagatacat tgccggcgcg aggaaggttt tcccgacggg aaatttgggc cacgggaatg
     4981 gaaccaccga cggttttcca ggcctcagca aaggaaacct cggcatcggc tacggctggt
     5041 ccgcggattt ctacccccgt gtctcgccag ggaggaatcc cccgggatgg gtctcccgcc
     5101 catgcatccc ccatgcacag tccggaaatg aaggccaaag aaccatctat ggtgagcaac
     5161 ttccgatgat cccggctggc ccaacccaac aaactgtcca tgcttggcgg gttggcagcg
     5221 cggacttcgg ccccggcctc gcgcgcaggc ttccacatgc gataggccag gggcccgtgg
     5281 gagcctaccc agtcgtataa aacccggacc ttgaccctct ggcgtgcctt ctccgttata
     5341 aggtcccgga accgccgccc cgtttggtcg ttgtgaataa tgtacatttc caggtggatt
     5401 gttttttggg cggcttcgat ggctttcagc cattctgggt agttctcctc cccgtcccgc
     5461 agaatgcgca gggaatttcc atgcaccagc ggggcccccg aggcccgcag gaaggcgtcc
     5521 gcggtcaggg ctcgtttcaa atcgatggaa gtcctaccca tgggtgcctc tggaaccttg
     5581 ccgctggcat tgccgttttc ttccaaggaa aagagatttg attttcattg gggggcctat
     5641 ttcttcaacg aaatctgcag ctccatcaga atgggcatat ggtcggaagc gatcaaagca
     5701 gacagagtcc ggggaacttc cactttcaaa acctcaacct gtccctggta gtagatatgg
     5761 tcgatgtgaa agatcggaag aaatccgggg taggtgcggc gccacttcag aaaagggagg
     5821 agagcgaggc gctgaaaggt ttcagccaga agccttgagg ccggtccttt cctccactca
     5881 ttaaaatccc ccagtaaaat ttttggccca cgaaccgatg ggtcggagag aaaatagatg
     5941 agctttctcg cttgccgctc tctttccttt tgggaagtgc cgaggtgcac attgtaaaga
     6001 tgaatcctat gaccctgaaa ggaaagatcg accctctgaa gaagacgagg ttccaggcca
     6061 tcttcgctca gatcgaaagc cgattgatgc ttgatgggaa tccggctcag aagggcattc
     6121 ccatagagat gcccccgcag cgtacgggcc ggggccagca cggaggccat acccagtcgc
     6181 tggctgatcc tctcctcctg cccaaaacct cgagggcctg ctccgatcac ttcttgcaag
     6241 gccacgatat ccgggttcag ccgtcccagc accgaggcga tccgttcagg gtgggtccgg
     6301 ccgtccattc ctcggcattt gtgcacatta taggtaacga tccgtaaccg gagtggttcc
     6361 tggctttggc tcatagacac attatagagg aaaaagggaa aaaagggagg gacccaagtc
     6421 aaacagtggt ctattccagt agtcgatctg catcctagcc gcatggccaa aaatcggctg
     6481 tggagaaatg aaattcttga ttatccgacc ggagggctaa tcgactgggc gactattttt
     6541 acatttcttt ataataatcc ttattcaatt cctcttcgag cagcctggcg tgcctcagtt
     6601 cttctttggc caaggattcg aacatttccc gggttccggg atatttggcg attttcgcgg
     6661 ccttggtata gaattcaaag gcagcctttt cccgctgcat ggcaaggcga atggcttctt
     6721 ccggggtcgt gctatcgtca atcatcctgg cctcctgaat ggttcaacca cggataggac
     6781 tttgtatcct tttttctgca aatcagtcac gagggggatc accgggtatt tcctcacccg
     6841 gaaatagtaa acccgttttt ttcgatcttt gtgattggac atgcctaaac tgataatctc
     6901 ggtcttgtgt ttccggaaaa tggccgatac ctcctcaaag gcatgggctc ttccccccaa
     6961 gaccacatca atccgggaac ttgatttcaa gattcccaga agctggataa aggcggccag
     7021 gatatccacg atggtgagga tcccgacaag ttttttcccg gccacgaccg gtagcccgcc
     7081 gattttgtgc cggtagatga gcttggccgc ttcttcgata ctgtcttcgg gggaaacggt
     7141 gaccggtttc ttgatcatca cgtcttcgat agagacttta tccaccaaag atagaagaga
     7201 cgcctgccgc aggtcaccct cggtaaccag cccgaccagg tctccttcct ctaccacggg
     7261 gaggtgccgg atggattttt cgtgcatgat ctcaatcgcc cggagaatgc tggtatccgg
     7321 aggaatggtc acgacttttt tggacatctg atttttgact tgcatggcct tttccttcgg
     7381 gctggaaaac cggaattttg aaatgaatgt atcaaccctg cattcatttg tcaacagacc
     7441 aaaaataatt catttttcat tttgacagta catcccgctc atgctaaata ggcaaaagag
     7501 gggatttgaa gcggcggaaa ggccgtttag gaaaaggagt tcttatggct gatccattct
     7561 gggaaagaga aatcgagacc ctaaaaccca aagagctacg ccggctgcag cttagacgat
     7621 tgaaaaagac actgcgacag gccgccacat caccttttta ttctggtctt ttcaaaaaac
     7681 accaggtctc cgcggaaaag gtcgaatctc tggaggacct gaaggaaatt ccctttacca
     7741 ccaagcagga cctgagagat caattcccct atggtcttct taccgttccc cgggaaaaga
     7801 tcgtccgttt gcactcttct tccgggacca cggggcgggc cacagcggtc tttcatacgc
     7861 aaaaggattt ggatgggtgg gcgaacctgg tggcccgttg catgtatatg gccggggtcc
     7921 gcaaacaaga tgttttccaa aatatgagcg gctatggcct tttcaccggg gggttgggtt
     7981 tccagtatgg ggccgagcga ctcggggccc tgaccattcc ctccggctcg ggaaacagca
     8041 agcgccagat tcacctcatg caggattttg gcactaccgt cgtccatatc atccccagct
     8101 atgccctcca cctctggaaa gtattcgggg aaatgggcct cgaaccaaaa agagacaccc
     8161 aacttcggat tttgttgatc ggggccgaac cgcattcgga agccacccgc cagcggatcg
     8221 aagaaatgta cggagtggat gctttcaatt cgtacggcct ctccgagatg aatggacctg
     8281 gggtggcctt tgagtgcccc caaaaaaaag gaatgcattt ctgggaagat gccttcctcg
     8341 tggaggtcat cgaccccaaa accggggaag ttctgccgga tggagtggaa ggagaactgg
     8401 tctatacgac cctgaaccgg gaagccatgc ccatgatccg ataccgttcc aaagacttgg
     8461 caaccatctt tcccgagctc tgtccctgcg gaaggaccca ccgccgcatg agccggatca
     8521 aggggagaag cgacgacatg ttcatcctca aaggagtaaa catctttccc ctgcagattg
     8581 acacgaccct catgaatatc cctgaagtgg ggacgaatta tctgatcgtt ctggaaagag
     8641 aaaatttcga ggaccggatg atcgtccggg tggaggtccg gccccaattc tttaaaggag
     8701 acctgtggca gttggagaaa cttcgcaaac agattaccga ggccctgaag tccgaactcc
     8761 tggttacccc gaaagttgaa ctcgtggaac ccgacagcct ccccaagagc gagggcaaag
     8821 ccgtgcgggt catggacaaa cgggaaatgt aaatgagaac agaatccagg agtcagaagt
     8881 cagaattcag aaggggaaaa attgtcttca ttctggctcc tgggttctga cttctgaatt
     8941 ctgggttctg aattctgcaa ggagggctta tggcccatca aatttcggtt ttcgtcgaga
     9001 acaagccagg gcggattgag cgggtgaccg agtttctgat gaaggcggag gtaaatatcc
     9061 gcgccataac cgtcatggga accttcgagt acggggtgat caaactcctg gtggacaaac
     9121 ccgacatcgc cctggaggcc ctccggtccc aggggatatc ggcccacaag cgggagatca
     9181 tcgccatcct catggatgac cgtccggggg ggctgcaccg gatcgccaag gtcttcggcc
     9241 agaagaagat caacatcgac gatgcctacg gctttgtcat cgaggacaag aagcgggccg
     9301 ttctagtcgt ggatgtggga aaaatccagg aagcggagaa ggtcctgaag gaggaaggcc
     9361 ttaccatcct gagtgacaga gagatttatt cgctttgatg aggaaaaggt agagagaatt
     9421 catgaattga ccctacttca tgatttttgt acagaaggtc cttgcccccg ggcctggcac
     9481 ttggtagaaa acggccttta ggtggtgccc atttcagtcc gctttgagtg gttcactgtt
     9541 tctcgtacct ccacctctgc tcgaggtcat gattggattc gaaaagaggg ggattagcgg
     9601 gcccttttca attcaatcgt gaccgcattc gtccgatcca tacactccaa ttcgacctta
     9661 tcgggcccgc cccattccat cgggaagccg cccccaaact ggagcatgag gatgtaggga
     9721 aaggcatcgt ggtagaaaaa gccgcacatc ccggccgtgt tatggccgct gagttcaagt
     9781 tgctgccccg ctttgtgccc ggcgttgcaa tgacccttga cgctcttaat ggtcccgacg
     9841 acgcgatagc cgattccctt gacttcagcc atgatcgtgc ctcctttgga atgaccctat
     9901 ggggaaactc atttgagtat tgaggaactg ttttgattcc tagcgttgat atctctgtgc
     9961 tttctgcgca atctccggaa taaacagttt tcggaattcg tccttgggat tcaaaggcct
    10021 caggcctctt tacacaattc ttcccagaac tttagaaatt gttctttatt gtatttgggc
    10081 ttataggcag atttgatggc cagtccttcc cgggcgccgt cccagaggag gtctatgacc
    10141 gtcatggcca atcccttggc ggtttcgatg caggcgaggc gggtgtcttc gatgcaaaaa
    10201 ctttcggtgt gcaccttagc gctcccggct ttgatcatgg ggtgcaatcc gggcatgaga
    10261 tgggtgatgt ctcccatgtc cgaagagccg gtgaggtggg ggctccggcc tatagcctcc
    10321 gggccgagga gataggccat gttggcggcg aaaagcccgt ctaaccgctc actgttctgc
    10381 atgggaagat agccggggag gttacggatt tccacctcgg cgcccacggc catggcgcca
    10441 gccttgagag cccggttgac ctttcggtgg gcatcctgga aagcctctgc ggtccggcct
    10501 cggacaaagg tctcgatgcg gacatctccc ggcactacgt tgaccaggtc tcctcctttg
    10561 gtaaggatgg gatgaacccg gatgctgtcc tcatccttga aagtctccct ctgggcgtgg
    10621 attcccatga gtccgaggag cgctgcattc aaggcattga cccccagatg gggagccccg
    10681 ccggcgtggg attctttgcc cttgtagcag atgaatttgg ccaggcagcc gttcatcgtg
    10741 acccccacgg ccactttctg agggggctcg gcgagctccg tgtggaacat gagggacaca
    10801 tcgatggcgt cgaaaagccc caggcggatg aattcctgct taccggttag aaaggcgatc
    10861 tttccttcct ccctgagctt gttgcggtac tcgagttcaa ccagctcttc tgccgggaca
    10921 gccatgagag aaacgtcccc gtccagctct ttcattacac cggagtcaag caggcccatc
    10981 cctgctccga gcatgccggc gatctgggcg ttgtgtccgc acccgtgggc cgctccggtc
    11041 agcggatcgg caaagggatg gacgggtatg cggatggaat ccagttcgcc catgaccgcc
    11101 acctggagtt ttttcgactt gccggcaata tgggcgacga ccccggtcaa agcgagcccc
    11161 tttttatagg acaaacccat ggagcgaaat ttatcctcga ccagggcggc ggtcttcact
    11221 tccttgtaac ccaattcggg gttgcgtaga atcgtctccg cgatttcagt gatttccttg
    11281 gcccgctttt cgacagaacc aatcaccttt ttcttcaagt cttccttggt aggcatgatt
    11341 cctccgattg gatggatatc cggtatccag catccagtat ctaatattta gcttttcacg
    11401 gtttccagcg cagttttggc ttccggttgg ccaggacttc atccaggcgg aagactttgg
    11461 ttcgatgggg agcgctgcga accacctccg gatcagtctc ggcttccttg gcgatttgaa
    11521 tcatggattc aataaactgg tccagagtct ccttggcctc gctctccgta ggctcgatca
    11581 ttagcgcccc gtggacaacc agggggaagt aaatggtggg gggatggaag ccataatcca
    11641 tcagtctctt agctatgtcc agggcggtaa ccccgaattt gttctgcacc ttgtcgttga
    11701 agacgcactc gtgcatgcag ggaaaatcgt aggggaggct gtaggtgcct ttcagccgag
    11761 ctcggatgta gttggcattg agcagggcca gcaggctggc tttcttgaga ccctctgggc
    11821 ccatggagag aatgtaggaa taggcccgga ccaggacccc gaagtttccg tagaaggagc
    11881 ggaccttgcc gatggatttg gggcggttgt attcgaaacg gaagcgctct ccttctttgg
    11941 ccacaacggg aatgggaaga tagtcggcca ggtgaccctt caccccaacc gggccggcac
    12001 ccggaccgcc tcccccgtgg ggagtggaaa aagttttgtg aaggttgaaa tggaggacgt
    12061 cgacgcccat atcccccacc cgggtcaatc ccaagagggc gttcaggttg gcaccgtcgc
    12121 agtagatcag gccgcctctc ttatgaacga tttccgcgac ttcgcagatg tggtcttcga
    12181 aaagaccgag ggtattgggg ttcgtaacca tgagcgcggc cacatcctca tccatcttct
    12241 cttcgattgc tcggggggat aaccttcccc gctcgtcaga tttcacctga acgacctggt
    12301 acccgcagag gctggaactg gccgggttgg tcccgtgagc cgtgtcggga accaggacct
    12361 ttttccgcgg attcccccgg tccatcaagt acgcacggat cataagcatc cccgtcagct
    12421 cgccctgagc tccggcggag ggctgcaggg ttactcgatc catgccgctc acttcaccca
    12481 gataatgttc cagttcatag ataagctgaa gctctccctg agagacctcc tctggctggt
    12541 agggatgggc cccggcaaag ccgggcagac gggctatatc ttcattgacc ttggggttgt
    12601 atttcatagt gcaggagccc agagggtaaa acccagaatc gaccccgtag ttccactggg
    12661 agaggcgggt gaaatggcgg acc
//
LOCUS       DASPRR010000113         4839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1965231, whole genome shotgun sequence.
ACCESSION   DASPRR010000113 DASPRR010000000
VERSION     DASPRR010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965231"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VEL68_04040"
     CDS             complement(<1..1389)
                     /locus_tag="VEL68_04040"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXZ35173.1"
                     /translation="MAAKEKESNRTKDLEKKLKFQGKLAWDRLEERERKEVFRFAEGY
                     KKFLDRAKTEREAVLEILQAGKRAGYRDLSRGGGGNQFFSVNREKSIGLALLGKAPLA
                     EGLRVIVSHIDSPRVDLKQNPLYEESDLALFRTHYYGGIKKYQWVAIPLSLHGLIVKG
                     DGSMLPIQVGEDSDDPVLVINDLLPHLSAKTQDQKKLSEGIEGEKLTALAGSLPYPDG
                     EAKERIKLRLMELLHEKYGLVEEDFTSAELELVPAMKARDVGFDRSLIGAYGQDDRAS
                     AYTSLQAVLSLKNPFRPVLALFADKEEIGSEGNTGAQSLFIEEVVREMIERSGDSPSD
                     AMVKRVLARSKAISADVESALDPNYPEVHEKQNAGRIGYGVCLEKFTGHRGKAGASDA
                     RAEYVGEIRRIFNTQNVAWQMTEIGKVDEGGGGTVAKYLAVYGMDIIDCGVPVLSMHS
                     PFEVTSKVDIYEG"
     gene            complement(1443..1655)
                     /locus_tag="VEL68_04045"
     CDS             complement(1443..1655)
                     /locus_tag="VEL68_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35174.1"
                     /translation="MMEFTLSKKPLQNNPSCAYCGNVFNEKGLSLQIQVDHKVVDFPL
                     CQRCFDQMPRLQATVNLENGSARVKR"
     gene            complement(1652..3085)
                     /locus_tag="VEL68_04050"
     CDS             complement(1652..3085)
                     /locus_tag="VEL68_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ35175.1"
                     /translation="MGVQARMKKFTTVQTAKAILKVLPRISEEKLLRLSIVQKGLDAV
                     SYYPEGRDFLKSLLLQGRRAMPRSSKSCLTKFAENLIVNEFIAATPKREEFRSRYGFD
                     PPFFLVLSPTMRCNLTCYGCYAGQYEKGAELDTDLIHRVLREAKEMGIYFVTVSGGEP
                     FIRPDLLDIFATHEDMYFQVYTNGTFIDSKMAKTLSRLGNVLPAISVEGWEKETDARR
                     GPGAFQKILDTMTRLKEEGVLFGFSATATRQSNELIISDEFVNFFAERGCFLGWYFNY
                     IPIGKAPDLSLMPTPEQRMFRWQRLMELRRRAPMVLVDFWNDGALVGGCIAGDRYLHI
                     NCRGDVEPCVFIHFSIDNIKKKSLAEILNSGFFQAIRRRQPYTQNYFRPCLIIDHPHL
                     LRQVVEESGAHPTHPGAETILTQFSGEMKEYASAYGKLADDLWKEQDPSVVLAYLRRR
                     FPSADKLEAGGYRPGKLKPVLKKEENR"
     gene            complement(3144..3848)
                     /locus_tag="VEL68_04055"
     CDS             complement(3144..3848)
                     /locus_tag="VEL68_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXZ35176.1"
                     /translation="MFRTSLCYLVFFITTVFLGTLAIFLSLFDSSGNLSHRVARLWGK
                     VQLRTTGTRVQVQGLENIDSHRSYILVSNHQSTFDIFALLGYLPIQFRWTAKAELFRT
                     PFMGWSMSRIGYIPIERDSPKKAYRSMLRAAEVVRKGMSVIIFPEGTRSPDGNLQPFK
                     KGVFLIALKSQAPILPITIQGTSRIMRKGDWRAYPGNVRIQIDPPIDTAEIPAAKEEQ
                     LSQRVRSTLVKNLTPT"
     gene            4070..4474
                     /locus_tag="VEL68_04060"
     CDS             4070..4474
                     /locus_tag="VEL68_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXZ35177.1"
                     /translation="MVNKVILIGRLGADPEIRYTPSGAEVANFRIATSEMWTNKNGEK
                     EERTEWHRIVAWRNLAKICGEYLSKGRLVYIEGKIRTRAWEDRDGNKRSTTEIEATEM
                     KMLGGSGEAERKTKEPDADYAPPAPKEEDIPF"
BASE COUNT         1034 a         1314 c         1404 g         1087 t
ORIGIN      
        1 cccttcgtaa atatcgactt tggaagtcac ctcgaagggg gagtgcatgg atagcaccgg
       61 aaccccgcag tcgatgatgt ccatgccgta gacggccaga tacttggcca ccgtccctcc
      121 tcctccctca tccaccttgc cgatctcggt catctgccag gccacgtttt gggtgttgaa
      181 gattcgccgg atttccccga cgtactcggc ccgggcatcg gaagcccccg ctttcccccg
      241 gtgaccggta aatttctcca ggcagactcc gtagccgatc cggcctgcat tttgcttttc
      301 gtggacctcc gggtagttgg ggtccagcgc cgattccaca tccgcggaga tggccttaga
      361 acgggccagc acccttttga ccatggcgtc ggaaggggag tctcccgacc gttcgatcat
      421 ttcccggacc acttcttcga tgaaaaggga ctgggccccg gtgtttccct cgctgccgat
      481 ctcttccttg tcggcgaaga gagccaggac cggccggaac gggtttttca gggaaagtac
      541 cgcctgaagg gaagtgtagg cgctggcccg gtcatcctgc ccgtaggccc cgatcaaact
      601 tcggtcgaat cctacatccc tggccttcat ggccggcacc agttccagtt cggcactggt
      661 aaagtcctct tcgaccagtc cgtatttttc atggaggagc tccatgagcc gcagcttgat
      721 ccgttccttg gcttctccgt cggggtaagg gaggctgccg gccagagcag tgagcttttc
      781 cccctcaatg ccttcggaca acttcttctg atcctgcgtt ttggcggaga gatggggcag
      841 aaggtcgttg atgaccagaa ccgggtcgtc ggaatcctcg ccgacctgga tgggaagcat
      901 tgagccgtcc ccctttacga tcaatccatg aagggacaga gggatggcca cccattggta
      961 ttttttgatc cccccgtagt agtgggtccg aaaaagggcc aagtcggact cttcatagag
     1021 cgggttctgc ttgaggtcca cgcggggaga gtcgatgtgg gagacgatga cgcgcaggcc
     1081 ttcggccagc ggagccttcc ccagaagggc caggccgatg gacttttctc ggttgacgga
     1141 gaagaattga ttcccacctc cgccgcggga aagatcgcgg tagccggctc ttttacccgc
     1201 ctgaaggatt tccaggaccg cttcccgctc ggtcttggcc cgatccagga actttttata
     1261 tccctcggca aagcgaaaaa cttctttcct ctctctttcc tccaatcggt cccaggcaag
     1321 ctttccctgg aacttcagtt ttttctccag gtccttggtt cgattcgatt ctttctcttt
     1381 ggccgccatg ttcaattctc cctctcctca ggatgaaccc gcgatcggat gcggaaccct
     1441 gcttaccgtt tgacccgggc cgacccgttc tccaggttca ccgtcgcttg aaggcgcggc
     1501 atttgatcaa agcagcgctg gcatagggga aaatctacga ccttgtggtc aacctgaatc
     1561 tgcaggctca ggcctttttc gttgaacaca ttcccgcaat aagcgcagct agggttgttc
     1621 tgcaggggtt tcttgctcag ggtaaattcc atcatcggtt ttcctccttt ttcaacactg
     1681 gtttcagttt gccaggccga tatccgcctg cctccagctt gtccgctgac gggaatcttc
     1741 tccgcaggta agctaggaca acggacgggt cctgttcttt ccacagatcg tccgccagtt
     1801 ttccataagc cgaggcatac tctttcattt cacccgaaaa ttgagtgaga atggtctccg
     1861 ccccgggatg tgtgggatga gctccggatt cctccaccac ctgacggaga aggtggggat
     1921 ggtcgatgat caaacaggga cggaagtaat tttgggtgta gggctggcgc cggcggatcg
     1981 cctggaagaa cccggagttc aggatctcgg ccagcgattt ctttttgata ttgtcgatgg
     2041 agaagtggat aaagacacac ggctccacgt cgccccggca gttgatgtgc agataccgat
     2101 cccctgcgat gcatcctccc accagcgctc cgtcgttcca gaaatcgacc aggaccatag
     2161 gagccctccg gcgcaattcc atcagccgct gccaccgaaa catccgctgt tccggcgtag
     2221 gcatgagact caggtcgggg gctttcccga tggggatgta gttgaaatac cagccgagga
     2281 aacacccccg ttcggcaaag aaattcacga attcatcgct gatgatcaat tcgttactct
     2341 gccgggtcgc ggtggcggag aatccgaaga ggactccctc ctctttcaac cgggtcatgg
     2401 tgtccaggat cttttgaaaa gccccgggac cgcggcgggc gtcggtttct ttttcccatc
     2461 cttcgacgct gatggcgggg agaacattcc ctaaacggga gagggtcttg gccatcttgg
     2521 agtctatgaa ggtcccgttg gtgtacacct ggaaatacat atcctcgtga gtggcgaaga
     2581 tatcgagaag gtcgggacgg atgaaaggct cgcccccgga gacggtcacg aaataaatcc
     2641 ccatctcctt tgcctcgcgg agcacccggt gaatcaagtc cgtatccaac tctgcccctt
     2701 tttcatactg gccggcatag cagccatagc aggtcaggtt gcagcgcatc gtgggactga
     2761 gaaccaggaa aaagggcgga tcgaaaccgt atcgggagcg gaattcctcc ctcttgggag
     2821 tggcggcaat gaactcatta acgatgaggt tctcggcaaa tttggtcaaa cagcttttgg
     2881 agcttcgggg catggccctc cggccttgaa ggagaaggct tttgaggaaa tctctccctt
     2941 ccgggtaata ggaaacggca tcaagcccct tttgcacgat ggacaatcgg aggagctttt
     3001 cttcgctgat ccggggaagg accttcaata tggctttcgc cgtttgaacc gtagtaaatt
     3061 ttttcatccg ggcttgaaca cccattccct ggcatcctcc ttgggatgga aattagtgta
     3121 ttctaaaaga attcccgagt caatcaagtc ggggtgagat ttttgaccag ggtactgcgg
     3181 actcgctggg agagttgctc ttctttcgcg gcgggaattt cggcggtatc gatggggggg
     3241 tcgatttgga tccggacgtt cccggggtag gcacgccagt cgcccttgcg catgatccgc
     3301 gaggttccct gaatggtaat ggggaggatg ggggcctggg atttcagggc gatgagaaaa
     3361 accccctttt tgaagggctg caaatttccg tccggactgc gggtcccttc aggaaagatg
     3421 atcactgaca tccctttccg tacgacttcc gcggcccgaa gcatgctgcg ataggctttc
     3481 ttcgggctat cccgctcaat ggggatgtac ccgatccgag acatggacca acccatgaag
     3541 ggagtgcgga atagctcggc tttggccgtc cagcggaatt ggatgggcag gtatcccaga
     3601 agggcgaaga tgtcaaaggt gctctggtgg ttggaaacca ggatgtagct cctgtgggaa
     3661 tcgatgttct ccaacccctg gacctggacg cgagttccgg tggtgcgaag ttggaccttt
     3721 ccccaaagac gggcgaccct atgggagagg ttcccggaag aatcaaagag agaaaggaaa
     3781 atggcgagcg ttcccaggaa caccgtggtg atgaaaaaga ccaaatagca gagagacgtc
     3841 cggaacatga attcccttcc ttcccaaagc tttaaaggaa gtctattcat actcgattct
     3901 aggaatgctg tcaactgggc tttcgaattt tccaataaag ccttgacttc cttcccttca
     3961 atgataaaat tcgcttcaat gatggaatcg gggaatccgg cggaaaccca ggaatttgag
     4021 gacggatcaa gccaagttct agggaagaca cggagaagga gggatcggag tggtcaataa
     4081 agtgatcctc atcggcagat tgggagcgga cccggagatc cgttataccc ccagcggagc
     4141 ggaagtggcc aattttcgga tagccaccag tgaaatgtgg accaacaaaa atggggagaa
     4201 agaagagcgg accgaatggc accggatcgt cgcctggagg aacctggcga aaatttgcgg
     4261 cgagtatttg agcaagggga gattggtgta cattgaaggg aagatccgca cccgggcctg
     4321 ggaagatcgg gacggaaata agcggtccac tacggagatc gaagccacgg aaatgaagat
     4381 gctgggagga tcgggcgaag cggaacgaaa aaccaaggaa ccggatgcgg attatgctcc
     4441 tcccgccccg aaggaagagg atatcccctt ctaatttcat tctggtaaac ccctggctct
     4501 gccgggggac ccaaagagtt tgacatttcc gggatgcctg agttgtggcc cgggggcaag
     4561 agtctggggt tcaaaaagca tcggccgggc gatccctatt cctggaggct gggactggct
     4621 tccgcgtgag ccggcggccc gatcgggcga ccggccggtc gcccctgcaa ggggaagacc
     4681 ttccaggggt tgcgggaccg gctggccgac ccggcccatg aaatgaagga cgcctcatgg
     4741 tttttgaacc ccagaccctt gcccccgggc ctggcattgg tgtccaggtg atggccacct
     4801 tcaggtggcg ctcctttcaa tccgctttga gcggttcag
//
LOCUS       DASPRR010000114         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2289536, whole genome shotgun sequence.
ACCESSION   DASPRR010000114 DASPRR010000000
VERSION     DASPRR010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289536"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="VEL68_04065"
     CDS             complement(<1..1660)
                     /locus_tag="VEL68_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HXZ35178.1"
                     /translation="MGWTHKKRMITTIRGGMPDQIPFAPRLDLWFAANKKRGTLPAPY
                     RGFEHYDLVSRAEGWGITRVVLDYQGYGEEAILDRALGVYQIPAQSFFTRLPLNVERR
                     VKKEGDRVLLEYATPKGSVHAAFVYSEEMRRSGVTIPWISEHALKGPRDYQPLAYIFE
                     NLVVEPVPELFHRWAAPLGEDGYAVAYALTAGSPMHHIMKILTDSTDFYYQHRDHADQ
                     MQVLAEGIGVFYRRVLAAVADGPGEIALVGANFDDTITYPPFFRDHILPWMQEASRLL
                     HAKGKWMLCHTDGENKGLMDYLLVSGMDIADSVCPAPMTKVSLAEYYDRWGKGITIQG
                     GIPSNLLLSEATTDEDFEDYLDRLFKAVAPGNRLILGVADTVPPDAVFDRLRRIAERV
                     EKEGRLPLEAGGFRPVAAETASYAPPPGKKLAGEDARFEVLRQDLYEGLDGEIKGHIR
                     ELLDRKVSPQEILNRGLLAAMESIGQEFKSGRLFVPEVLLAARAMNEGLSVLEPHLSS
                     SSRQARGKALIGTVQGDLHDIGKNMVIIMLRGVGFDVVDLGINVS"
     gene            2340..>2551
                     /locus_tag="VEL68_04070"
     CDS             2340..>2551
                     /locus_tag="VEL68_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35179.1"
                     /translation="MKWKPPHGFCTEGLCPRALGTEIPFPATQRENKTGGGQAKIRSI
                     ASRLMGLPLKEAEPSLPDGGNQDLRM"
BASE COUNT          520 a          748 c          770 g          513 t
ORIGIN      
        1 cggagacgtt gatgcccaag tcgacgacgt cgaatcctac tccccgcagc atgatgatga
       61 ccatgttttt cccgatgtcg tggaggtccc cctggacagt gccgataagc gctttccccc
      121 gggcttggcg gctcgaggaa gagagatggg gttccagcac ggaaaggccc tcgttcatgg
      181 cccttgccgc caggaggact tcgggcacga ataaccggcc gctcttaaac tcctggccga
      241 tgctctccat ggctgccagc aggcctcggt tcaaaatttc ctggggactt actttccggt
      301 cgaggagttc ccggatgtgg cccttgattt ctccgtccag accttcatag aggtcctgcc
      361 tgaggacctc gaaccgggcg tcctccccgg ccaatttctt cccgggcggg ggcgcatagg
      421 aggcagtctc ggcggctacc gggcggaacc cccctgcctc caggggcaac cgcccttcct
      481 tttccacccg ctcggcgatc cgccggagac ggtcgaatac ggcgtccggc ggcacggtgt
      541 cggccactcc cagaatgagt cgatttcccg gggccaccgc tttgaaaaga cggtcgagat
      601 agtcttcgaa gtcttcgtcg gtcgtggcct cagaaagcaa caggttggaa gggatccctc
      661 cctggatggt gatccccttg ccccaccgat cgtagtattc ggccaggctg actttggtca
      721 tcggggccgg gcagaccgag tcggcgatgt ccattccgct gacgagcagg tagtccatga
      781 gccctttatt ttctccgtcg gtgtggcaca gcatccactt ccccttggcg tggaggagcc
      841 gggaggcttc ctgcatccag gggaggatgt ggtcgcggaa aaaaggaggg taggtgatgg
      901 tatcgtcgaa gttggctccc accagggcga tctcccccgg accatcggcc accgcagcca
      961 gaacccggcg gtaaaagacc ccgatgccct cggccaggac ctgcatctga tctgcatggt
     1021 cccggtgttg gtaataaaaa tccgtgctgt cggtgaggat tttcatgatg tgatgcatgg
     1081 gagaacccgc ggtcagggcg taagccaccg cgtacccgtc ctcgcccagg ggggccgccc
     1141 atcgatgaaa cagctcggga accggttcga ctacaagatt ctcgaagata taggccagcg
     1201 gttggtagtc ccgcggaccc ttgagggcgt gctcggaaat ccaggggatg gtcacgccgg
     1261 agcggcgcat ctcttccgag tagacgaaag cagcgtggac cgaacccttg ggggtggcat
     1321 attccagaag gactcggtcc ccctcctttt tcacccgccg ttccacgtta aggggaagac
     1381 gggtaaaaaa gctctgagcc gggatttggt agacccccag ggcccggtcc agaatggcct
     1441 cctcgccgta tccctgataa tcgaggacca ccctggtgat cccccacccc tcagcgcgcg
     1501 agaccaggtc gtagtgctca aagccgcggt agggtgcggg tagggtcccg cgcttcttgt
     1561 tggcggcaaa ccataggtcc aaacggggag cgaaaggaat ctggtccggc attccaccgc
     1621 gaattgtggt gatcatcctt tttttatggg tccagcccat gggccctcct gaaaccccca
     1681 tggcagataa agtttctttc gggtatggaa gaaatcttaa gaggggggaa tatgaatcgc
     1741 ccccgcgcct atgtaaaaag tttagtcgat aggagagagg atgacaacag aatttggcga
     1801 gtcgccaacc gggctgagcc aggattcggt tttgcaaaag ggggtcgggg accagcgctt
     1861 tgcggaacac gagcagcagc tccggaatcc gtatttttcc cgccgattct gccccgccat
     1921 gggcaggacg caccccctgg gcccctttcc atcgactcga ttcggagaag gctcccttta
     1981 aatactggaa gatggaagct ggaaattcaa tgacaaaata tcctatgccc accccgactt
     2041 cttgataaaa gaccacttcg atcgcgcctg cccaaaggaa atctgcggag ggggatagtt
     2101 gccgtttccc agggatttgc cgcgggtcca cctcacgcgg attcacattt ccgggatgct
     2161 tcggttgtgg cccgggggca aagaccttct gcgcaaaaag catcggcagg gctggccctc
     2221 ctctgggagg cttcgacctg cttccgtagg atacggcggg ctgaacggac gcctggccgg
     2281 gtgcccctac aacagaataa tcttgtcctt ggggagagaa gggttggccc gcccgggcca
     2341 tgaaatggaa gccgcctcat ggtttttgca cagaaggtct ttgcccccgg gccttaggca
     2401 ccgaaatccc cttccctgca actcaacgtg agaataaaac gggaggtggg caagcaaaaa
     2461 ttcgctccat cgcttcacgc ctgatggggc ttccgctcaa agaagcagag ccatcattgc
     2521 cggacggggg gaatcaggat ttgcgaatgg a
//
LOCUS       DASPRR010000115         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1806067, whole genome shotgun sequence.
ACCESSION   DASPRR010000115 DASPRR010000000
VERSION     DASPRR010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806067"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VEL68_04075"
     CDS             <1..218
                     /locus_tag="VEL68_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HXZ35180.1"
                     /translation="LWKGYERGEFTDSMIYAELGEVVCGRKKGRETRNERTLACLTGV
                     GTLDVAVARQVYERALKRKLGLWVSLW"
     gene            363..1370
                     /locus_tag="VEL68_04080"
     CDS             363..1370
                     /locus_tag="VEL68_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ35181.1"
                     /translation="MGRRIVFLALVFLTFGVMEMIPQVWAAETVTVYSSNMQALNDLA
                     AAEFEKATGIKVNMVRAPSGVALKRMRAEKDNPQGDVFWGVSKVVLMANSDLLEPYKS
                     VYYDQIPAEFRDPEYRWIGTNLQILVLMYNKNLVKDTDAPKKWNDLLDPKWKNKVAFP
                     NPANSSFAFTAFTLMLHMYGNNDAAWKKMESFLGNMQVVEQSAMVPGALEKGEFHVGI
                     TQEYVASRYVAGGAKVGMIYPEDGNSVQAEGAGIIKGAKNRKQAEKVVDFFNDKKFRE
                     LVIKDQFRRPARKDLDFSKIVPMPPLSQIRLMPGYVDTNYVNDRDKILARIKDILLRI
                     K"
     gene            1443..>2059
                     /locus_tag="VEL68_04085"
     CDS             1443..>2059
                     /locus_tag="VEL68_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35182.1"
                     /translation="MSIRIVGVSKRFGSLEAVKNVSLEVHTGEFFTLLGPSGCGKTTL
                     LRSIAGFNQPEAGEIYFGERRIDKMSAHKRGIGMVFQNYAVFPHLTVNDNVAYGLKAR
                     KIPTQEVAPRVTKALQMVRLEGMGGRLPNQLSGGQLQRVAIARALVIEPQVLLMDEPL
                     SNLDAKLRVEMRSEIRELQRQMQITTVYVTHDQEEALSISDRIAV"
BASE COUNT          520 a          503 c          603 g          433 t
ORIGIN      
        1 aactatggaa aggatatgaa aggggagaat tcaccgactc gatgatttat gccgaactcg
       61 gagaggttgt atgcggaagg aagaaaggca gggaaacgag gaatgagagg actctggcct
      121 gtctgactgg tgtcggaacc ctggacgtgg ccgttgcccg gcaagtgtac gaaagggcct
      181 tgaaacgcaa attggggctc tgggtcagcc tttggtaaag ccgccccatc aatgcaaaat
      241 gcggaataat gccagtcttt tcttatcccg cattccgcat ttgagggctt tcccttaacc
      301 ttgacacccc tgatcaaaaa agttagcatg atttgaattc gaccttgatt caggaggttc
      361 ccatggggag aaggatcgtt ttcctagcgc ttgtctttct gacgttcggc gtgatggaga
      421 tgattcccca ggtttgggct gcggagacgg taaccgttta ctcctctaac atgcaggccc
      481 tgaacgacct ggcggccgcg gagttcgaaa aagccacagg gatcaaggtc aacatggtac
      541 gcgctccttc gggagtggcc ttgaaaagga tgagggcgga aaaagacaac cctcaggggg
      601 atgtgttttg gggggtcagc aaagtggttc tcatggccaa ctccgacctt ctggaaccat
      661 acaaatctgt ttattacgat cagattcctg ctgaattccg ggacccggag tatcggtgga
      721 ttggaaccaa cctccagata cttgttctca tgtacaacaa aaatctggtt aaagacacgg
      781 atgctccgaa aaagtggaat gatctgctgg accccaaatg gaagaataaa gtggccttcc
      841 ccaacccggc gaactcctcc tttgccttta ccgccttcac tctgatgctc cacatgtacg
      901 gcaacaacga tgcggcctgg aagaagatgg agagtttcct gggcaacatg caggttgtgg
      961 agcagtcggc catggtgccc ggggccctcg aaaaggggga attccacgtg gggatcaccc
     1021 aggagtatgt tgcctcccga tacgtagccg gaggggcgaa agtagggatg atctatcccg
     1081 aggatggaaa ttccgttcag gccgaagggg cgggaatcat caaaggagcg aaaaaccgga
     1141 agcaggctga aaaggtcgtc gactttttta atgataaaaa attccgagag ctggtaatta
     1201 aggaccagtt tcggcggccg gcacggaaag acctggattt ctccaagatc gtacccatgc
     1261 cgcctctctc ccaaatcagg ttgatgcccg ggtacgtgga tacgaattac gtcaatgacc
     1321 gggacaagat cctggcccgc atcaaggaca ttctgctgag gatcaagtaa gtgccggctc
     1381 agcatggaag ggccttctgg gcggaagatc aggaggccct atttttttgt cagaggggaa
     1441 aagtgagtat ccggattgtc ggcgttagca aaagattcgg aagtctggaa gcggtcaaga
     1501 atgtgagcct ggaggttcac acgggcgagt tcttcaccct ccttgggccg agcggctgtg
     1561 gaaagacgac tctccttcgc agtatcgccg ggttcaacca gccggaagcg ggggaaatat
     1621 acttcggcga aaggcggatc gacaagatgt ctgcccacaa aagggggatc gggatggtct
     1681 ttcagaacta cgccgtcttt ccccatctca cagttaatga caacgtggcc tatgggctga
     1741 aagcccgtaa gatcccgacc caggaggtcg ctccaagagt gaccaaagca ctccagatgg
     1801 ttcgtctgga gggcatgggg ggacgccttc ccaatcagct ttccggcggc cagctccagc
     1861 gggtggccat cgcccgggcc ctggtgattg aaccccaggt attactcatg gacgagccgc
     1921 tgagcaactt ggacgccaag ctaagagtgg agatgcgtag cgagataagg gaactacaga
     1981 ggcaaatgca gatcaccacc gtttatgtca cccacgacca ggaagaggcg ctttccatct
     2041 cggaccggat tgcagtcat
//
LOCUS       DASPRR010000116        15283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1318979, whole genome shotgun sequence.
ACCESSION   DASPRR010000116 DASPRR010000000
VERSION     DASPRR010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15283)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15283
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318979"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VEL68_04090"
     CDS             complement(<1..125)
                     /locus_tag="VEL68_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXZ35183.1"
                     /translation="MSKKKFERTKPHVNVGTIGHIDHGKTTLTSAITKCLAQKGLA"
     gene            complement(144..2252)
                     /gene="fusA"
                     /locus_tag="VEL68_04095"
     CDS             complement(144..2252)
                     /gene="fusA"
                     /locus_tag="VEL68_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXZ35184.1"
                     /translation="MANRGHLEKTRNIGIMAHIDAGKTTTTERILYYTGVSYKMGEVH
                     EGTAVMDWMEQEQERGITITSAATTCFWREHRINLIDTPGHVDFTIEVERSLRVLDGA
                     VAVFCAVGGVEPQSETVWRQADKYHIPRIAFVNKMDRLGADYPRVVRMIRERLNARPL
                     VLQLPVGQEEKFRGVVDLLRQKAILYHEETLGAKFHEEPIPEELKPQVEIYREKIFEA
                     LAEADDPFMERYLAGETFSLEEIQALIRRETVAARLVPVVCGSGFKNKGIQPLLDAVV
                     DYLPSPLDVPPIRGHHPDTGLEEMRTPLNGAPLSALAFKIMSDPYVGQLTFLRVYSGL
                     LLSGSSVFNSNKGKRERVGRLLKMHANKREEIKEAGAGDIVAAVGLKETMTGHTLCAE
                     DHPLVLEAMEFPDPVISMAIEAKAKADQEKMDLGLHKLSMEDPSFRMFMDPETGQRII
                     SGMGELHLEIIVDRLMREFGIPVSVGRPMVAYRETITQKARAEGKFVRQSGGRGQYGH
                     VILEVQPLPPGEKFQFVDAIRGGTIPREYIPAVEKGVKEAAGNGVLAGYPVVDVKVTL
                     LDGSFHEVDSSEMAFKIAGSLGFKEGTKRAHPILLEPIMAVEVVVPESYVGEVTGDLS
                     SRRGKILNLESRNGLRILEARVPLAEMFGYATQLRSSTQGRATYTMQFSRYERVPAAM
                     AEEAIQQARGTEGAGRRRPG"
     gene            complement(2359..2829)
                     /gene="rpsG"
                     /locus_tag="VEL68_04100"
     CDS             complement(2359..2829)
                     /gene="rpsG"
                     /locus_tag="VEL68_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXZ35185.1"
                     /translation="MPRRREVTKRETLPDPKYKNALVARFVNNIMERGKKSVAEGIFY
                     GAMDLIQKRTQEDPLKLFEKSVGNVKPVIEVKSRRVGGATYQVPTEVRPERRTALAIR
                     WLISYAGERSEKSMQEKLAGELIDAANNRGGAIKKREDVHKMAEANKAFAHYRW"
     gene            complement(2837..3208)
                     /gene="rpsL"
                     /locus_tag="VEL68_04105"
     CDS             complement(2837..3208)
                     /gene="rpsL"
                     /locus_tag="VEL68_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXZ35186.1"
                     /translation="MPTINQLVRLGREKMKSRMTAPALTHCPQRRGVCIRVYTSTPKK
                     PNSALRKVARVRLTNGYEITTYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHVIRGTL
                     DSAGVQNRKKSRSKYGAKRPK"
     gene            complement(3272..7387)
                     /gene="rpoC"
                     /locus_tag="VEL68_04110"
     CDS             complement(3272..7387)
                     /gene="rpoC"
                     /locus_tag="VEL68_04110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXZ35187.1"
                     /translation="MEDLFSFFEKPKHPLSFNAVRISLASPEKIRAWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPTKDYECNCGKYKRMKHRGVVCEKCGVEVIQSKVRRERL
                     GHIELATPVAHIWFLKSLPSRIGSLLDMTLRELEKVLYFEAYLVVNPKNTSLKEKELL
                     NDERYRKALEEFGPDSFVAGMGAEAIRELLRKVDVQRLSEQLHQEMKEATSDVKKKKF
                     AKRLKVLDSFKDSGNKPDWMILEVIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLQELNAPEIIIRNEKRMLQEAVDALFDNGRGGRAILGSNGRPLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPDLRLHQCGLPKKMALELFKPFIYNRLEQKGYV
                     TTIKSAKKMVEQEKDEVWETLSEIVREYPILLNRAPTLHRLGIQAFEPILIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHIPLSIEAQIEARVLMMSTNNILSPAHGKPIIVPTQDIV
                     LGLYYLTREKPFVKGEGRNFSGPEEVRMAYDAGEVDLQAAVKVRLGTNGTQELVSTTV
                     GRILLREIVPPEIPFSFVNRVMNKKELANLIDQCYRYAGNKKTVILADQLKDLGYRYA
                     TRAGISICIDDMVIPARKAEILGKANREVLEIESQYKEGLITDGERYNKVVDIWAQVT
                     ENIGFEILEELGNVVITDPSGKKKRVPSFNPIFMMADSGARGSAQQIRQLAGMRGLMA
                     KPSGEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQD
                     LVVTEHNCGTLDGIPVTPLVEGGEIIEPIGERVLGRVALEDIVDPFTGEILVKADEEI
                     DERLVEKIENAGLEKVKIRSVLTCRSKRGVCALCYGRDLARGQLVNMGEAIGIIAAQS
                     IGEPGTQLTMRTFHIGGTASRRAEQTALQPRNDGRVKFLNVSTVSSKEGDLVVMNRNG
                     ELAIVDESGRERERYPVIYGAKIKVENGKAVKSGDLIAEWDPYTIPILTEVSGRVKFG
                     DIVEGVTMQEQLDEVTGFSTKVIIESKDPEARPRVSIKDEKGRTMKIPDAESMARYLL
                     PVGAHIVVTEGDKVHQGDVIAKIPRETTKTKDITGGLPRVAELFEARKPKEYALISEI
                     TGTVSFGRDTKGKRKVLITPEVGDPKEYSIPKGKHVSVHEGEKVKAGEALMDGSSNPH
                     DILAILGVEDLARYLVDEVQEVYRLQGVKINDKHIEVIVRQMLRRVVIKEVGDSNFLV
                     GEHVERHLFEEENDHLKSQGKQPATAEPLLLGVTKASLSTESFISAASFQETTKVLTQ
                     AAISGKTDYLRGLKENVILGRLIPAGTGLARYRNLGIQVEGGEEEMENSAK"
     gene            complement(7400..11578)
                     /gene="rpoB"
                     /locus_tag="VEL68_04115"
     CDS             complement(7400..11578)
                     /gene="rpoB"
                     /locus_tag="VEL68_04115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXZ35188.1"
                     /translation="MASWGSGQPIVRLNFSKIEKIIETPNLIDVQRKSYESFLQKDVP
                     PEERQDMGLQAVFKSVFPIRDFNETASLEFASYSLGEPKYEVDECRQRGMTFAAPLKV
                     IVRLINYEVDEEERKGRPRVRDIKEQEVYFGEIPLMTDKGTFIVNGTERVIVSQLHRS
                     PGILFEHEKGRTHTGKPLYSARIIPHRGSWLDLEFDTKGLLYVRIDRRRKFPVTILLR
                     SLGYSAEDLLNFFYRTEKVFFKDDKVYKNLDFDLLVNKRATEDIRHPQSGEVVVRKNR
                     KITAGALKRLSEAKVKALTLEVAELAGRYLARNVFHPQTGELLLPCNTELNKERVEEL
                     RAQGVKEVELLFIDHLNVSSSMRDTLAADRIEDTEARAIEEYRRQHPQDQTPRSIYNA
                     MLEVYRRLRPSEPPTLDTAKTFLENLFFNSDRYDLSRVGRLKINHRLKRSSTPESRVL
                     QREDILEAVKYLIELKDGMGEVDDIDHLGNRRVRAVGELLENQYRIGLVRMERAIKER
                     MSLQDAETLMPHDLINPKPVSAVVKEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPG
                     GLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLIASLSTYAKVDEYGFMVTPYRKV
                     VNGQVTKEIEYLSALQEDETRVIAQANAPVDAKGRFTAEMIQARIAGESTLVQPEEIN
                     YMDVSPNQLVSVAASLIPFLESDDANRALMGSNMQRQAVPLLRAESPLIGTGMEGTVA
                     RDSGVAVVAKRDGVVEKVDSSRIIIRAADNTGRNGSRSEVDIYNLIKYQRSNQNTCIN
                     QKPIVHEGQRVKKGQIIADGPATSNGELALGRNVLVGFMPWGGYNFEDSILISERVVK
                     DDSFTSVHIEEFEVVARDTKLGKEEITQDIPNLGDESLKNLDESGIIRIGAEVKAGDI
                     LVGKITPKGETQPSPEEKLLRAIFGEKAGAVKDTSLRVPPGVEGVVIGTKIFSRRGVE
                     KDIRAKAIEEEEIARILRDQEDEVQIVQQIAAQKVRKLYLNEVLTHRVAEERSGKTLI
                     PKNTKLTKEMLAEVADHRMRDVNIQNPKKKAEAEKVWEGLDERVQLIRGAFERKIARL
                     KKADELPPGVIKMVKVDISIKRKLSVGDKMAGRHGNKGVISRILPEEDMPYLADGTQV
                     DIVLNPLGVPSRMNVGQILETHLGWAAQGLGEQIEEIIRKEFNPKALREKVRKIYSSD
                     SFSRVIEEMTDEELLELARSLSDGVFMSSPAFDGATEKEIKHLLKEAGRTVQVTLYDG
                     RSGEPFDQKVTVGVIYMMKLHHLVDDKIHARSVGPYSLITQQPLGGKAQFGGQRLGEM
                     EVWALEAYGAAYGLQEFLTVKSDDVLGRTRMYETIVKGGHALDAGVPESFNVLVKELQ
                     SLCLDMELLEKEEA"
     gene            complement(11639..12022)
                     /gene="rplL"
                     /locus_tag="VEL68_04120"
     CDS             complement(11639..12022)
                     /gene="rplL"
                     /locus_tag="VEL68_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXZ35189.1"
                     /translation="MADLQKLVEELSGLTLLEAAELSKLLEQKWGVSAAAVAVAAVPG
                     AAAGGAAAPVAEEKTEFDAILATVGEKKIQVIKVVRELTSLGLKEAKDLVEGAPKPLK
                     QGISKEEAETVKKKMEEVGATVEIK"
     gene            complement(12065..12589)
                     /gene="rplJ"
                     /locus_tag="VEL68_04125"
     CDS             complement(12065..12589)
                     /gene="rplJ"
                     /locus_tag="VEL68_04125"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXZ35190.1"
                     /translation="MKREEKKERIADLQRQMGRAKVAILTRFSGLNVEKMTQLRRELR
                     KAAVEYQVVKNTLFRLAARGTDKEGLSSHLEGPLGVAWSDSDPVAPARVLAKYAKEWP
                     ELKVLVAASEGKIWGPADIQAWVSLPGLDALRAKILGLLQAPATTLVRLMGVPATQVA
                     RVLQGRSQQNEAVI"
     gene            complement(12773..13480)
                     /gene="rplA"
                     /locus_tag="VEL68_04130"
     CDS             complement(12773..13480)
                     /gene="rplA"
                     /locus_tag="VEL68_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXZ35191.1"
                     /translation="MSTRGKKYSEAKKKVDRTKRYDLDGGIQLLKETGQAKFDESVDM
                     AIRLGVNPKHSDQMVRGTVALPHGVGKSVRVLVFAKGEKEKEAKDAGADFVGAEDLVE
                     KITAGWTDFDKAIATPDMMGTVGRLGKVLGPRGLMPNPKVGTVTFDVARAVKELKAGR
                     VEFKVDKTGIVHTTVGKISFPAEKLKDNVMALMDVIQRAKPASSKGTYMKSIAISTTM
                     GPGIKLDPNDLRVATKT"
     gene            complement(13496..13924)
                     /gene="rplK"
                     /locus_tag="VEL68_04135"
     CDS             complement(13496..13924)
                     /gene="rplK"
                     /locus_tag="VEL68_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXZ35192.1"
                     /translation="MAKKVVGLVKLQIPAGQANPSPPVGPALGQHGVNIMEFCKAFNA
                     QTKNMEGTIIPVVITIYADRSFSFVTKTPPAAVLLKKAAGIIKGSGEPNRTKVGQVTR
                     AQVKEIAQTKLPDLNTTRLDSAVRTVEGAARSMGIEIIEG"
     gene            complement(13940..14470)
                     /gene="nusG"
                     /locus_tag="VEL68_04140"
     CDS             complement(13940..14470)
                     /gene="nusG"
                     /locus_tag="VEL68_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXZ35193.1"
                     /translation="MSQKWYVVHTYSGFENKVKSVLEERVHTAKKEDFFSEVLVPAEK
                     VIEMIKGKKKASSRKFFPGYILVKMELNEETYHLVKETPKVTGFVGDRSNPAPIPDAE
                     VEEIKNQMSEGLLRPKHRMTFEKGDQVQVVDGPFSNFNGLVEAVNPDKGKVRVLVSIF
                     GRSTPVELDFAQVKKG"
     gene            complement(14487..14690)
                     /gene="secE"
                     /locus_tag="VEL68_04145"
     CDS             complement(14487..14690)
                     /gene="secE"
                     /locus_tag="VEL68_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXZ35194.1"
                     /translation="MEKIKEFMRTAQQFFREVRVELKKVTWPTRKETIASTSVVLITV
                     FLVAFYLGVVDLGLSRLIKIFLE"
     gene            complement(14726..14802)
                     /locus_tag="VEL68_04150"
     tRNA            complement(14726..14802)
                     /locus_tag="VEL68_04150"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14766..14768),aa:Trp,seq:cca)
     gene            complement(14891..15040)
                     /gene="rpmG"
                     /locus_tag="VEL68_04155"
     CDS             complement(14891..15040)
                     /gene="rpmG"
                     /locus_tag="VEL68_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXZ35195.1"
                     /translation="MREIVTLACAQCKRRNYTTTKNKRSTPDKLEFKKYCRFCRTHTP
                     HKETK"
     gene            complement(15141..>15283)
                     /gene="tuf"
                     /locus_tag="VEL68_04160"
     CDS             complement(15141..>15283)
                     /gene="tuf"
                     /locus_tag="VEL68_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096692.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXZ35196.1"
                     /translation="EMVMPGDNVNIEVQLITPIAMEKELRFAIREGGRTVGAGVISDI
                     LE"
BASE COUNT         2779 a         4756 c         4066 g         3682 t
ORIGIN      
        1 gctaacccct tctgcgctaa acacttcgtt atcgcactcg ttaacgtcgt cttcccatga
       61 tcaatatgcc caatcgtccc cacattcaca tgcggtttcg tccgctcaaa cttcttcttc
      121 gacataaatc ctccttcact gggtcatccg gggcggcggc ggccggcccc ttcggtaccg
      181 cgggcctgct ggatcgcctc ctcggccatc gccgcgggca cgcgctcata acgggaaaac
      241 tgcatggtat aggtggcccg gccctgggtg ctcgaccgaa gctgggtggc gtagccgaac
      301 atctcggcca agggcacccg ggcttccaat atccgcaatc cgtttcggga ttcgaggttc
      361 aggattttcc ccctccggga cgagaggtct ccggtcacct ccccgacgta cgattcggga
      421 accacaacct ccacggccat aatgggttcc aggagaatgg gatgggccct cttggtccct
      481 tccttgaagc ccaaggaccc ggcaatcttg aaggccattt ccgaggaatc cacttcatga
      541 aaggaaccgt cgagcaaggt gacctttacg tccaccaccg ggtatccggc caggactcca
      601 ttgccggcag cctccttgac ccctttttcc accgcaggaa tatactctcg tggaatggtt
      661 ccccccctga tggcatcgac gaactgaaac ttttctccgg ggggaagggg ttggacttcc
      721 aggatcacat ggccgtactg cccgcgtccg cccgactggc ggacaaattt cccttccgcc
      781 cgcgccttct gggtgatggt ctcccggtag gcaaccatgg gcctgcccac gctgaccgga
      841 attccgaact ctctcatcag ccggtctaca atgatctcca ggtgcagttc ccccatgccg
      901 gaaatgatgc gctgccccgt ttctgggtcc ataaacatcc ggaaagaggg gtcttccatg
      961 gaaagcttgt gcaggcccag gtccattttc tcctggtcgg ccttggcctt ggcctcaatg
     1021 gccatggaga tcaccgggtc cggaaattcc atggcttcca ggaccagggg atgatcttcg
     1081 gcacacaggg tatggccggt catcgtctcc ttcagaccca cggcggccac gatgtccccg
     1141 gcgccggcct ccttgatctc ttcccgtttg ttggcgtgca tcttcaggag acggccgacc
     1201 cgctcccgct tgcctttgtt ggaattgaaa accgacgacc ccgacaagag caaccccgaa
     1261 tagacccgca gaaaggtcag ctgtcccaca tacgggtcgg acatgatctt gaaggccagg
     1321 gccgacaggg gcgctccgtt cagcggggtg cgcatctcct ccagcccggt gtccggatga
     1381 tgtccccgga tgggggggac atccagggga gaaggaaggt aatccaccac tgcatccaga
     1441 aggggttgaa tccccttgtt cttaaacccc gagccgcaga ccaccggcac cagccgggcg
     1501 gccacggtct cccggcggat cagggcctga atctcctcca gggaaaaggt ctcgccggct
     1561 aaataccttt ccataaaagg atcgtcggcc tcggccaaag cctcgaaaat cttctcccgg
     1621 tatatttcca cctggggctt cagttcttcg ggaatcggct cctcgtgaaa tttcgccccc
     1681 aaggtctcct catggtacag aatcgccttt tgtcgcagaa ggtcgacgac cccccggaat
     1741 ttttcctcct gtccaacggg gagttggagg acgaggggcc gggcgtttaa cctctcgcgg
     1801 atcatacgga cgacccgcgg gtaatcggct cccagccgat ccatcttgtt gacgaaagcg
     1861 atccggggga tgtggtattt gtccgcctgc ctccacaccg tctcgctctg gggttccacc
     1921 ccccccacag cgcaaaagac cgccaccgcc ccgtccagaa cccttaagct tcgctccact
     1981 tcaatggtga aatccacgtg gccgggggtg tcgatgagat tgatccggtg ttcccgccag
     2041 aagcaggtgg tcgccgccga agtgatggtg atgccccgtt cctgctcttg ttccatccag
     2101 tccatgaccg cggtcccctc atggacctct cccatcttat aggagacccc cgtatagtag
     2161 aggattcgct ccgtggtggt ggtcttaccg gcatcgatat gcgccatgat gccgatattt
     2221 cgagtttttt ccaggtgtcc tcgattggcc aaggctttcc atcctcaatc aaaggaaaaa
     2281 cgcccccgcg ccatgaaccc gccccttgcg gcggaatcca gggctcgtct ccgtcaagtt
     2341 ccttttcgtt ttttggttct accagcgata atgggcgaag gccttgttgg cttcggccat
     2401 cttatgaaca tcctcgcgct tcttgatggc tcctccgcgg ttgttggccg catcgatcaa
     2461 ttccccggcg agtttttcct gcatgctctt ctccgatcgc tcgccggcat aggaaatcag
     2521 ccatcggatg gccaaagcgg ttcgtctttc gggacggact tcggtgggaa cctgataggt
     2581 cgcacccccg acgcgccggg atttcacttc aataaccggt tttacgttac cgaccgattt
     2641 ttcgaagagc ttcaaggggt cttcctgggt tcgcttctgg atgaggtcca tggctccata
     2701 gaagattcct tcggccacgc tcttctttcc ccgttccatg atattgttga cgaaccgggc
     2761 gaccaaagcg ttcttgtact ttgggtccgg aagagtctcg cgcttggtga cttcccgtct
     2821 tctgggcatg ggttcctcac ttgggtctct tggctccgta cttggagcgg ctcttctttc
     2881 ggttctgcac cccggcagaa tccagcgtcc cccggatgac gtggtaacgc acgccgggaa
     2941 ggtctttcac tcgtcccccg cggatcaaga ccacggagtg ttcctggagg ttgtggccca
     3001 cccccggaat gtaggtggtg atttcataac cattggtcag acgaacccgg gcgactttgc
     3061 gcaaggccga attcggcttt ttgggggttg aggtataaac ccgaatacaa accccgcgtc
     3121 gctggggaca gtgagtcaga gcgggggcgg tcatcctcga tttcatcttt tcacggccga
     3181 gtcggaccag ctggttgatg gtcggcatta aacctccaaa gagataaaat aatcttttta
     3241 gtctaccatc ttcaatcgat ttgtcaatcc actatttagc cgaattctcc atctcttctt
     3301 ctccgccctc cacctgaatc cccaggttcc ggtaccgggc taatccagtt cccgccggga
     3361 tcagtcggcc gagaatcacg ttctctttca gcccccggag gtaatccgtc tttccgctga
     3421 tcgccgcttg ggtcagaact ttcgtggtct cctggaagga ggcggcggaa atgaagcttt
     3481 ccgtgctcaa ggaagctttg gtcaccccta acaacagggg ttcggcggta gccggctgct
     3541 tgccctgaga ctttaaatgg tcgttttcct cctcgaagag gtgccgttcc acatgctctc
     3601 ccacgagaaa attggagtct ccgacctcct tgatgaccac ccggcggagc atctgccgca
     3661 cgatcacttc gatatgtttg tcgttgatct tgaccccctg gaggcggtac acttcctgca
     3721 cctcatccac caggtagcgg gccaggtctt ccactcccaa aatggccagg atgtcatggg
     3781 gattggacga cccatccatg agcgcctccc cagccttaac tttttctccc tcatgaacgc
     3841 tgacgtgttt ccccttgggg atcgaatact ccttggggtc tcccacctcc ggagtgataa
     3901 gcaccttgcg cttcccctta gtgtccctac cgaaggaaac ggttccggtg atttcactga
     3961 ttagggcgta ttccttgggt ttgcgggctt caaagagttc cgccacccgg ggaagtcccc
     4021 cggtaatatc tttggtcttg gtcgtttccc gagggatttt ggcgatcacg tctccctggt
     4081 ggaccttgtc cccctcggtg accacaatat gggccccaac cggcaggaga taccgggcca
     4141 tgctttcggc gtcggggatc ttcatcgtcc gccccttctc gtccttgatg gatacccggg
     4201 ggcgggcctc cgggtccttg gattcgatga tgactttggt ggagaatccg gtcacctcgt
     4261 cgagttgctc ttgcatggtg accccttcca cgatgtcccc gaatttcacc cttccggaca
     4321 cctcggtaag gatggggatg gtgtaggggt cccactccgc aatcaggtca ccgctcttca
     4381 ccgccttccc gttttcaacc ttgatcttgg ccccgtaaat gactggatac cgctcccttt
     4441 ctcgtccgga ttcatccaca atggccagct ctccgttgcg gttcatgacc accaagtccc
     4501 cttctttgct ggagactgtg gatacgttta aaaacttcac ccgcccatca ttccggggct
     4561 ggagggcagt ttgctcggcg cgtcggctgg ccgtcccccc gatgtggaag gtacgcatgg
     4621 tcagctgggt tcccggctcc ccgatggact gggcggcgat gatcccgatg gcctccccca
     4681 tgttgaccaa ttgcccccgg gccaagtccc gcccatagca aagggcgcaa accccccgct
     4741 tcgaccggca ggtcagaacg gaccggatct tgaccttctc caacccggcg ttttcgatct
     4801 tctccaccag acgctcgtcg atctcctcgt cggctttgac cagaatctcc ccggtaaagg
     4861 ggtcgacgat atcctccagg gccacccggc ccaggacccg ctccccgatg ggctcgataa
     4921 tctcccctcc ctccaccaag ggagtcaccg gaattccgtc cagggtcccg cagttatgct
     4981 cggtcactac caaatcctgg gctacatcca ccagcctccg ggtgagatag cccgaattgg
     5041 ccgttttcag cgccgtatcc gccagacctt tgcgggcccc gtgggtggag ataaaatact
     5101 ggagcacggt caaaccttca cggaagttgg cggtgatggg agtttcgata atttctccgg
     5161 agggtttggc catcagcccc cgcattcccg cgagctgccg gatctgctgg gcgcttcccc
     5221 gggctcccga atcggccatc atgaagatgg ggttgaacga ggggacccgc ttctttttcc
     5281 cgctgggatc ggtgatgacc acattgccca gttcttccag gatctcgaag ccgatgtttt
     5341 cggtcacctg ggcccaaata tcgaccacct tgttgtaccg ttccccatcg gtgatcaacc
     5401 cttctttgta ttggctctcg atctccagaa cctcgcggtt ggctttcccc agaatctccg
     5461 ccttccgggc ggggatgacc atatcgtcga tgcagatcga gattcccgcc cgggtggcat
     5521 accggtaccc taaatctttg agctgatcgg ccaggatgac agtcttttta ttgcccgcat
     5581 agcggtagca ctgatcgatc agattggcca attctttctt gttcatcacc cggttcacga
     5641 aggagaaggg gatctccggc gggacgattt cccgcagcag gattcgtccc acggtggtgg
     5701 agaccaattc ctgcgtcccg ttggtcccca ggcggacctt gaccgccgcc tgaaggtcta
     5761 cttctcccgc gtcgtaggcc attcggactt cttctggacc agaaaagttc cttccctccc
     5821 ccttcacaaa gggcttttcc cgggtcaggt agtagaggcc gaggacgatg tcctgggtgg
     5881 gtacgatgat cggctttcca tgagccgggg agaggatgtt gttggtggac atcatcaaca
     5941 cccgggcttc gatctgggct tcgatcgaca gggggatgtg aacggccatc tgatcgccgt
     6001 caaagtcagc attgaacgcc gcgcagacca gaggatggag ctgaatggcc ttcccctcga
     6061 tcaggatggg ttcgaaagcc tggattccca ggcgatggag ggtgggggcg cggttcagca
     6121 agatgggata ttctcggaca atttcgctca gggtctccca gacttcgtcc ttctcttgct
     6181 ccaccatctt tttggcgctt ttgatggtgg tcacataccc tttctgttct agacggttgt
     6241 agataaaggg cttgaataat tccagagcca tctttttggg caacccgcat tggtggaggc
     6301 ggaggtcggg gcccaccacg atcaccgaac gaccggaata atccactcgt tttcccaaca
     6361 ggttctgtcg gaagcgccct tgttttccct tcagcatgtc gctcagggac ttgaggggcc
     6421 tgccgttgga acccaggatc gcccgtcccc cgcgaccgtt gtcaaaaagg gcgtccaccg
     6481 cctcctggag catgcgcttc tcgttgcgga tgatgatctc tggagcgttc agttcctgga
     6541 gccgcttcag ccggttgttc cggttgatga cccggcggta taggtcgttc aggtcggagg
     6601 tggcgaaccg cccgccgtcg aggggaacca gaggccgaag gtcgggggga atcacgggga
     6661 tgacctccag gatcatccaa tccggcttgt tccccgaatc cttgaaggag tccagcacct
     6721 tcagtctttt ggcgaacttt ttcttcttca catcggaggt ggcttctttc atctcctggt
     6781 ggagctgttc ggagagccgc tggacgtcca ccttccgcag gagttcgcgg atggcttcgg
     6841 cccccattcc ggccacgaaa gagtccggcc cgaattcctc cagcgccttg cggtagcgct
     6901 cgtcattgag cagttctttc tctttgaggg aggtgttctt gggattgacc accaggtagg
     6961 cttcgaaata gaggaccttc tccagctctc gcagggtcat atccagaagg ctgccgatgc
     7021 gactgggcag gctcttcagg aaccaaatgt gggccaccgg ggtggccagc tcgatatgcc
     7081 ccagccgctc cctccgcacc ttggactgga tcacctccac gccgcacttt tcgcagacca
     7141 cgccgcgatg tttcatcctt ttgtacttgc cgcagttaca ctcgtaatct ttcgtggggc
     7201 caaagatctt ggcgcagaaa aggccgtccc gctccggctt gaaggtccgg tagttgatgg
     7261 tctccggctt cttgacctct ccgtacgacc aggcccggat cttctcgggt gaagccaggg
     7321 aaattcgaac ggcattgaag gacaaagggt gtttgggttt ttcaaaaaaa ctgaaaaggt
     7381 cttccaatac ggacctcctt taggcttcct ctttctccag aagctccatg tccaggcaaa
     7441 ggctctgcag ctcctttacc agaacgttga aggactcggg gactcccgca tccagggcgt
     7501 gccccccctt gacgatggtc tcatacatcc gcgtccggcc gaggacatca tcggatttta
     7561 cggtaaggaa ttcctgcagc ccgtaggccg ctccataggc ttccagggcc catacttcca
     7621 tctcccccag acgctgtccg ccgaactggg ccttcccgcc caagggctgc tgggtgatca
     7681 gggagtaggg tcctaccgaa cgggcgtgga tcttgtcgtc caccagatga tgaagcttca
     7741 tcatgtaaat gactcccacg gtcaccttct gatcgaaggg ttctccgctg cgcccgtcat
     7801 agagggtgac ttggaccgtt cgtcccgcct ctttcagcag atgtttaatt tccttctccg
     7861 tggctccgtc aaaggccggc gaggacataa atacgccgtc ggaaaggctc cgggcgagct
     7921 ccaggagctc ctcgtcggtc atctcctcga tcacccggga gaaactgtcc gagctgtaaa
     7981 ttttccgcac cttttcccgc aaggctttgg ggttaaactc cttgcggatg atctcctcaa
     8041 tctgctcccc cagcccctgc gccgcccatc ccaggtgggt ttccaggatc tgtcccacgt
     8101 tcatacggga ggggacgccc agagggttga gaacgatgtc cacctgggtc ccgtcggcca
     8161 gatagggcat gtcctcctcg ggaaggattc gagagatgac ccctttattc ccgtgccgcc
     8221 cggccatttt gtcccccacc gagagtttgc gtttgatgga aatatccacc ttcaccattt
     8281 tgatcactcc cgggggcagt tcgtcggcct ttttcaggcg agcgatcttc ctttcgaaag
     8341 ccccccggat gagctggacc cgctcatcca gcccctccca gaccttctcc gcctcggcct
     8401 ttttcttcgg gttctggatg ttcacgtccc gcatccggtg gtccgcaacc tcagctagca
     8461 tctccttggt gagcttggta ttcttaggga tgagggtctt gccgctccgc tcctctgcca
     8521 cccggtgggt gaggacctca ttcaagtaga gttttcggac cttctgggcg gcgatttgct
     8581 ggacgatctg gacttcatcc tcctggtccc tcagaattcg agcgatctct tcttcctcta
     8641 tggctttggc ccggatatcc ttttccactc cccggcggct gaagatcttc gtaccgatca
     8701 cgaccccttc gactcccggc ggcacgcgga gcgaggtatc cttcaccgcc ccggcctttt
     8761 ccccaaagat ggcccgcagg agcttctcct ccggagaggg ttgcgtttcc cctttggggg
     8821 taatttttcc cacgaggata tcccccgcct tcacctcagc cccgatgcgg ataatgccgc
     8881 tctcgtcgag gttcttcagg gactcatccc ccagattggg aatatcctgg gtgatttctt
     8941 ccttgcccag tttggtatcg cgggccacca cttcaaactc ctcgatatgc accgaggtga
     9001 aactgtcgtc cttcaccacc cgctcgctga tcaggatgga gtcctcaaag ttgtacccgc
     9061 cccagggcat aaagcccacc agaacgttgc gtcccagggc cagctctccg ttgcttgtgg
     9121 ccggaccatc ggcgatgatc tggcctttct tgacccgctg tccctcatgg acgatgggct
     9181 tctgattaat gcaggtattc tgattcgacc gttggtattt gatgaggtta tagatatcca
     9241 cttcggagcg ggacccgttc cggccggtgt tgtcggcggc ccgaatgatg attcgggagg
     9301 aatccacctt ttccaccacc ccgtcccgct tggccaccac cgctaccccc gaatcccggg
     9361 ccaccgtccc ttccatcccc gttccgatca ggggggattc ggcccgcagc aggggaaccg
     9421 cctggcgctg catgttggac cccatgagcg cccggttggc gtcatcgctt tccaggaagg
     9481 ggatgagcga agcggccacg ctgaccagct gattggggga gacatccatg taattgattt
     9541 cctccggctg caccagcgtg gactcgccgg cgattcgtgc ctgaatcatt tccgcagtga
     9601 accgcccctt ggcatccacc ggggcattgg cttgagcaat gacccgggtc tcatcctcct
     9661 gcaaggccga gagatattcg atttccttgg tgacctggcc gttgaccact ttgcggtagg
     9721 gagtcaccat aaatccgtat tcatccactt tggcataggt gctcagggaa gcgatcaacc
     9781 cgatgttggg accttccggg gtctcgatag ggcagacccg gccatagtga gtcggatgga
     9841 cgtcgcgcac ctcgaagcca gcccgctccc gggtcagccc cccgggcccc agcgcggaga
     9901 gacgccgctt gtgggtgatt tcggacaggg gattggtctg gtccatgaac tgggagagct
     9961 gcgaagaccc gaagaactcc ttgaccactg cggatacagg cttgggattg atcaggtcat
    10021 ggggcatgag ggtttcggcg tcctgaaggc tcatccgctc cttgatggcc ctttccatcc
    10081 gcaccagccc gatccggtac tgattctcca ggagttcccc cacggctctc acccgccggt
    10141 ttcccaggtg gtcgatatca tccacttccc ccattccatc tttcaactcg atcaggtatt
    10201 tcaccgcttc caggatgtct tcccgctgca aaacccggct ttccggcgtc gaacttcgtt
    10261 tcaggcggtg attgatcttc agccgtccca cccgagagag gtcataccgg tcggagttga
    10321 agaaaaggtt ttccagaaag gtcttggccg tatccaaagt gggaggctcg ctggggcgca
    10381 gacgccggta gacctccagc atggcattgt agatggagcg gggggtttga tcctggggat
    10441 gctggcgacg atactcctca atggctcgag cctccgtatc ctcgatccgg tcggcggcca
    10501 aggtgtcccg catcgaggaa gagacattca gatgatcgat gaagagaagc tcgacctctt
    10561 tcaccccttg ggcccgcagt tcctccaccc gttccttgtt cagttccgta ttgcagggca
    10621 acaggagctc gccggtttgg ggatggaaca cattccgggc caggtagcgc cccgccagtt
    10681 cagcgacttc cagggtgagc gctttgacct tggcctcgga cagcctcttg agggctcccg
    10741 cggtgatctt tcggttcttg cgcaccacca cctcgccaga ttggggatgg cggatgtctt
    10801 cggtggcccg cttattcacc agaagatcga agtcaaggtt cttgtaaacc ttgtcgtctt
    10861 taaaaaatac cttttcggtg cgatagaaga agttgagaag gtcttcggcc gagtagccca
    10921 gggacctcag aagaatggtc accgggaatt tccgcctccg gtcgatgcgc acgtagagca
    10981 ggcccttggt gtcaaactcc aggtccagcc aggacccccg gtggggaatg atccgggccg
    11041 aatacaaggg tttgcctgta tgggtccggc ccttctcgtg ctcaaacagg atgccgggag
    11101 agcgatgcag ctggcttacg atgactctct cggtcccgtt gacgataaag gtccctttat
    11161 cggtcatcag gggaatttcg ccgaagtaga cctcctgttc cttgatatcc ctcaccctgg
    11221 gccggccctt ccgttcctct tcgtccacct cgtagttgat gagacgtacg atcaccttga
    11281 gaggggcggc aaaggtcatt ccccgctggc ggcactcgtc gacttcatac ttcggctccc
    11341 ccagggagta actggcgaac tccagggagg ccgtttcgtt gaaatcccgg atgggaaaga
    11401 cgcttttgaa aacggcctgc agccccatat cctggcgttc ctcgggggga acgtcttttt
    11461 gcagaaaact ctcataggac ttccgctgca catcgatcag gttgggggtt tcgataattt
    11521 tttcgatttt ggaaaaattc aagcgaacaa tcggctggcc gcttccccag gaagccattc
    11581 cttatttcct cctcatttcc ccccaagggg ggttgaaaac cgatgaaagg gcaggtcgct
    11641 atttgatctc cacggtggcc ccgacttctt ccatcttctt tttgacggtc tccgcctctt
    11701 ctttagagat tccttgtttc aacggtttcg gagccccttc caccaagtcc ttggcctctt
    11761 tcaaaccgag gctggtcagc tcgcggacga ccttgatgac ctggatcttt ttctctccca
    11821 cggtggcgag aatcgcgtcg aactcggtct tttcttctgc caccggcgcg gctgcgcccc
    11881 cggccgctgc ccccggaacg gcggccaccg ccacggcggc agcagacacg ccccattttt
    11941 gctccagaag tttgctcagt tcggctgctt ccagcagggt caaaccgctc aattcttcaa
    12001 ccagtttctg tagatcagcc attttttctc tccttgagga tgattttttg atcccgaaag
    12061 atcatcatat taccgcttcg ttctgctggc tccgcccttg gagcacccgg gccacctggg
    12121 tagccggaac ccccatcagt cggaccaggg tggttgccgg agcctggagg aggcccagaa
    12181 tcttggccct cagcgcgtcc aggccgggca gcgaaaccca cgcctgaata tcagccggtc
    12241 cccagatttt gccctcggat gcggccacca ggaccttaag ttccggccat tccttggcat
    12301 atttcgccag aacccgggcg ggagccactg ggtctgagtc gctccaagcg acccccaggg
    12361 ggccttccag atgggaactc aacccctctt tgtccgttcc ccgggcggcc agtcggaaaa
    12421 gggtgttctt gaccacctga tactccaccg ccgccttgcg caactctcgg cgaagctggg
    12481 tcatcttttc cacattgagg cccgagaaac gggttaagat ggccactttg gcccgcccca
    12541 tctgcctctg aagatcggcg atccgttctt ttttttcttc gcgcttcaaa cctcaacctc
    12601 ccttattatt tcgccgacct ccgagaggat cccgcaccgc agaatccgat gatccttccc
    12661 tgaatcttct cttcctcagg ttcccatccg tctcggtagg cgggcgaaat ccctttaagc
    12721 gttggctgcg acgtacctac ggtcttcgac gaaatccttc acccgttccg tatcaggttt
    12781 tcgtggccac ccgcagatca ttggggtcta gcttgatccc cggccccatg gtcgtggaga
    12841 tggcgatgct cttcatgtaa gtccccttgc tggatgcggg tttggcccgt tggatcacat
    12901 ccatgagggc catcacattg tctttcaatt tttcagccgg aaaggagatt ttccccaccg
    12961 tggtgtgaac gatgccggtt ttatccacct tgaactccac ccggccggct tttaactctt
    13021 tgaccgcccg agccacgtca aaggtgaccg tccccacttt ggggttgggc atcaatcccc
    13081 ggggacccag aaccttcccc agtcttccca ccgttcccat catatcgggg gtggcgatgg
    13141 ccttgtcgaa atcggtccag cccgcagtga tcttctccac taggtcttcg gcccccacaa
    13201 agtcagcccc cgcatctttc gcttctttct ccttttctcc tttggcgaaa accagaacac
    13261 ggaccgattt gcctacccca tgggggaggg ccacggtgcc ccggaccatc tggtcggagt
    13321 gcttggggtt cacccctagg cggatggcca tgtccacact ctcgtcaaac ttggcttggc
    13381 ctgtttcctt gagcagctgt atcccgccgt ccaggtcata acgtttggtc cggtcgactt
    13441 tctttttggc ttctgagtac tttttccctc gagtgctcat acctttaccc ctctattacc
    13501 cctctatgat ctcgatgccc atcgagcgcg ccgccccctc caccgtccga acggccgaat
    13561 ccaaccgggt ggtattcagg tctggcaatt tggtttgggc gatctctttc acctgggccc
    13621 gggtcacctg acccactttg gtccgattgg gctcccccga ccccttgatg atccccgccg
    13681 cttttttcag gaggaccgcc gccggagggg tcttggtgac aaaggaaaac gaacggtccg
    13741 cataaatggt gatcaccacg gggatgatcg tgccttccat gttcttggtc tgggcattga
    13801 aggctttgca gaattccatg atgttgaccc cgtgttgtcc cagagccggt cccaccggag
    13861 gcgacggatt ggcctgtcca gcagggatct gcaatttcac caagccgacg actttcttag
    13921 ccattttcgt cctcttcttc tatcccttct tcacttgggc gaaatcgagc tctacgggag
    13981 tggagcgccc gaagatactc accagaactc gaacctttcc cttgtccgga ttgaccgcct
    14041 ccaccagccc gttgaagttg gaaaaggggc cgtccacgac ctgcacttgg tctccctttt
    14101 caaaggtcat gcggtgcttg ggccggagca ggccttcgct catctggttt ttgatctcct
    14161 ccacctccgc gtcggggatc ggcgcgggat tggaccggtc gcccacgaat ccggtcacct
    14221 taggggtctc cttcaccaga tggtaggttt cttcgttgag ttccatcttt accagaatgt
    14281 aacccgggaa aaatttccgg gaggaagcct ttttcttccc cttgatcatc tcgatcactt
    14341 tctccgccgg gaccaaaacc tcggagaaaa aatcctcctt cttggctgta tgaacccgct
    14401 cttccaggac ggatttgacc ttgttctcga atccggaata ggtatgaacc acgtaccact
    14461 tctgggacat aggatttgtc cttgccttat tccaaaaaaa tcttgatcag ccgcgaaaga
    14521 cccaagtcca ccacccccag gtaaaaagcc accaaaaaca cggtgatcaa aaccaccgag
    14581 gtggaggcga tcgtttcctt gcgggtgggc caagtcactt ttttcagttc cactcgaacc
    14641 tctcgaaaga actgctgagc ggtccgcatg aattccttga tcttctccat gtccctcttc
    14701 ctggagctgg aaaatcccct tatcctggca ggccaggagg gattcgaacc cccatcaccc
    14761 ggatttggag tccggtgctc tagccgttag agctactggc ctgctctctc gcgggttttt
    14821 ccgctgatcg atggatcgac ctctgataaa ccggaggaag gtttatggtg ccgttggttc
    14881 tttccctgac ctacttggtc tccttatgcg gagtatgggt ccggcagaaa cggcagtatt
    14941 tcttgaactc cagcttgtca ggtgtagaac gcttgttctt ggtagtggta tagtttctcc
    15001 gcttgcactg ggcacaagcc aatgtgacaa tttctctcac aaggcccctc ttccttttga
    15061 taaacggcaa aggagttcaa ccctggggga tgaactctct ttcttttttg ggtcttcgcc
    15121 gggcttgggt cttcgggctc ctattccagg atgtcggaga tgactcccgc tcccaccgtc
    15181 cgtcccccct cccggatcgc aaaccgtaac tccttctcca tggcaatcgg agtgatcaac
    15241 tgcacctcga tattcacatt gtccccgggc atcaccatct cca
//
LOCUS       DASPRR010000117         7395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1154683, whole genome shotgun sequence.
ACCESSION   DASPRR010000117 DASPRR010000000
VERSION     DASPRR010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154683"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1620
                     /locus_tag="VEL68_04165"
     CDS             259..1620
                     /locus_tag="VEL68_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ35197.1"
                     /translation="MDSLTGKLASFITGLKYQSLPSEVIQKAKHCLMDTLGAALAGSK
                     TQEAALAKKVAQKLSQKKEATLITGKGKIGVLEAALANGIMAHALELDDGNRYALGHP
                     GVPTIPAVLALAEKEKKGGKEVISSIVAGYEIFGRVGAGGNPSHFNRGFHTTGTVGTF
                     AASAAAGRLLNLSEAKMVSALGIGGSQSAGLFAFMGDGTMTKTLHAGKAAMNGILAAY
                     LAKEGFTGPAYILEDKRGFYKAFADASNPDRVVEGLGQKYEIMNTYVKYHASCRHSHA
                     PVDAILDIRNRTGLRPEEIEKVNIYTYTIAAKLIDGKDVSSPISGKMSMPYASAVALL
                     YGRVGLGEFKPKVMSDPAVLGLMQKINVFPDPEMDKLIPHHRAARAEIFMRDGTKLTS
                     DILDAKGEPENPGASSDIFDKFRMLAGTVFKKDKVEKILERIDNLEKVKDMSELNNLL
                     TAK"
     gene            complement(1767..2315)
                     /locus_tag="VEL68_04170"
     CDS             complement(1767..2315)
                     /locus_tag="VEL68_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ35198.1"
                     /translation="MRKEIVFAGFGGQGIILMGVILAQMMEEFPELQVTQAQSYGPVS
                     RGGACRTDVVISDQEIHFPKSARPDFMVFMSEESVKRYLQEGDPDKTLVIYDSTLIRS
                     IPAPYQKTFPVPATKVAEEELKNRIVANMFMLGAAIEKVLPGSFAALQRVVGKSVPPK
                     SLDLNLRALEAGHAFARTRSPG"
     gene            complement(2312..2551)
                     /locus_tag="VEL68_04175"
     CDS             complement(2312..2551)
                     /locus_tag="VEL68_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ35199.1"
                     /translation="MKGAERIEIDRRYCKGCMLCQYVCKFEVFEAGQERSALDYRMPR
                     VAKMENCRVCGLCEINCPDLALTVVAKKKGKGKES"
     gene            complement(2548..3384)
                     /locus_tag="VEL68_04180"
     CDS             complement(2548..3384)
                     /locus_tag="VEL68_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014121913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ35200.1"
                     /translation="MNPLGEKYLRKNFLPFIFCPGCGDGMIVQAFLEAVDELGIIDDL
                     ALAAGIGCSAWIPVYMHVDVLKVLHGRAIPAAIGLKATRPSRKVVVFTGDGDGLAIGG
                     LHLLHAARRNIEMGVVVVNNQIYAMTGGQVAPSTQVGARTQTTPYGNLEKPLDGCKVA
                     VAAGATFVSRGSTAHPKALSRTFKEMLTHKGFSFVEVISQCPVQTGRYIHGSGAPQFN
                     LQWLKDNCVPVDDPAKWVEVEGQGKIPLGDVLKIENPEYMEEVQKMNQKAKEARAKGG
                     DR"
     gene            complement(3381..4517)
                     /locus_tag="VEL68_04185"
     CDS             complement(3381..4517)
                     /locus_tag="VEL68_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ35201.1"
                     /translation="MKKGCIKVMQGNEACVEAALLAGCRFFAGYPITPASEILEGMAR
                     RLPLVDGFCLQAEDEIAAIVAVVGASWGGAKAMTATSGPGFCLMQEGIGLAASVESPC
                     VIVNVQRGGPASGQSTSTAQGDLYQARYGSNGDYSLITLCPSTSQEVFDHTIRAFNLA
                     ERFRIPVIILSDEVVAHIREKVVIPDPDQLEFVERKKPTVPMEKFVPFEAGEDDVPPM
                     PAFNTGYKIPVISQAKSLSGNRGNAKEAESFIRRINRKVEAHIAEFADVRTYGDEKAE
                     TAFVSFGSVSRSARSAVDMAREKGVALRSVEVKTIWPFPDDLLRKRLEGAKRIIVAEM
                     NLGMLRNEVQRVICAPEVKIELFSKIGGIYPPPQEFLQFLEERR"
     gene            complement(4792..6237)
                     /locus_tag="VEL68_04190"
     CDS             complement(4792..6237)
                     /locus_tag="VEL68_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ35202.1"
                     /translation="MIEREKRIRKAELRNKITTAKEAAQFLRDGMVVATSGTALSGYP
                     REVFGALRERIKNGDSLRIDLLCAGPLGPEIEDALAEVGGIRKRIGTVGSKLLRQAIN
                     RGEVGFLEGKTGKVCQYARRGYYGRIDVAVVEAAGINEKGQIIPATCVYDAPDWLDLA
                     SSAIIEINLRRPLGVEGLHDIYYPEPGRCIPLKHPLERIGSPYISLDPEKIKHIIFSD
                     RDDRDVPAFPPGERVPQIAENIGKFLRREREQRGELPPLEVGIGDVLNGVLSSLTRSD
                     LSSLIFYQAVVTDPLLDLIDAGKVKGVSCSSLRFSASALNRFFSRLEHYRPYFIFRPV
                     TLTNSAEMLSRFGVLAINSGLEADIQGQINSSHSKGSQLIGGIAGSYDFARNSSISIF
                     ALPSTIQQGKVSSIVPRVSHVDHTEHEVDVLITEHGIADLRALEPYERAERIIEQCAD
                     PIFRGSLRTYVEKAKTHPGRIPILLQDEFKV"
     gene            complement(6304..7323)
                     /locus_tag="VEL68_04195"
     CDS             complement(6304..7323)
                     /locus_tag="VEL68_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ35203.1"
                     /translation="MPFDEVKWRERVEDQSVEKLYAAHFNGGKYFNPWMPMEHGKFWQ
                     VLKWQLSKKAQYTDEERAFRPKFIPDLKRRVQSLPPGDFIAWVGHSTFLLRLKGEFWI
                     TDPIFSERAFLPKRMTPPAMTGGELKDLTPRLNVVISHNHYDHLDADSIRSLPENTRF
                     YVPLGLKAYVESIHKGAVREMDWWEDADVGGGAKLVCLPAQHWSRRIGQGYNRTLWAS
                     FLLVTSETSIYYGGDSGYFIGYKEFGRKFPNIDYALLSTTAYHPRWFMHYAHKSVPEA
                     LDAFQDLGAKYFIPTQWGTFALGDEPPGYPALDLKRTVKQRNLDPSRFLILDVGQIEP
                     IRRSG"
BASE COUNT         1568 a         2183 c         2032 g         1612 t
ORIGIN      
        1 taaagaacct caaaacgagg tccggaggct catcccaccc tacttaaaaa ctacccaacc
       61 cctgcccctt tgtcaagatt tttgctgttc attggggttg tccattgtgc ttcccctccc
      121 cgattgactt aggcaggcaa cgatggttta atggcactga attaatgaaa tggagtctcc
      181 ccggattttc aatgtttaaa gcagaccgtt aagggattct tatattgaat cctaatctta
      241 aaggagggcc aagtcgaaat ggatagccta acaggaaagt tagcttcctt catcacgggt
      301 ctaaaatatc aatccttacc atctgaggta attcaaaagg ccaagcactg tctgatggat
      361 actctggggg ctgctctggc cggttcgaag acccaagagg cggcgttggc caaaaaagtg
      421 gcgcaaaaat taagccagaa gaaggaagcc accctgatca ccggaaaggg aaagattggg
      481 gtcctggaag cagccctggc caacgggatc atggctcatg ccctggaact ggacgacgga
      541 aaccggtatg ccctgggaca ccccggggta cccactatcc cggcggtcct ggccttggcc
      601 gaaaaggaaa aaaaaggggg gaaggaggtc atttcctcca tcgtcgccgg ttatgaaatc
      661 ttcggccggg tgggcgcagg gggcaacccc tcgcacttca accgcggatt ccataccaca
      721 ggaacggtgg gcacctttgc tgcctctgcg gcggccggtc ggctcttgaa tttgagcgag
      781 gccaagatgg tttcagcgct gggcatcggc gggtcgcaat ctgcgggcct ctttgccttc
      841 atgggcgacg gaacgatgac caagaccctc cacgcaggga aagccgccat gaatggaatc
      901 ctcgccgcct acctggccaa ggaaggtttc accggcccgg cttacatcct ggaagacaag
      961 aggggtttct acaaagcttt tgccgacgct tccaaccccg accgggtggt tgaagggctg
     1021 gggcaaaagt atgaaatcat gaacacctac gtgaaatacc atgcctcctg ccggcacagc
     1081 cacgctcccg tggatgccat cctggacatc cgcaaccgga cgggcctccg gcccgaggaa
     1141 atcgagaaag taaacatcta cacttacacc atcgcggcca agctgatcga cgggaaggac
     1201 gtctcctccc ctatctccgg gaagatgagc atgccctacg cttcggcggt cgctctcctg
     1261 tatggaaggg taggcctggg agaattcaag cccaaggtca tgagcgatcc ggctgtcctg
     1321 ggattgatgc agaagatcaa cgtcttcccc gatccagaaa tggataagct gattccccac
     1381 caccgcgccg cccgggcgga gattttcatg agagacggga ccaagctgac ttccgatatc
     1441 ctggacgcca aaggggagcc ggagaatccg ggtgcgagca gtgatatctt cgacaaattc
     1501 aggatgctgg cgggaacggt cttcaagaag gacaaggtgg agaaaattct tgaaaggatc
     1561 gacaacctgg agaaagtcaa ggatatgtct gagctgaaca acttactgac agcgaagtag
     1621 ccctgctcca accatttcgc tcagtgttca accgcccctg tttctcttat atgaggagca
     1681 ggggcgattc tttttcattg cccctccctt cctaagccca cagaacccca atccagatcc
     1741 tgcccgggaa gaggctgatg aaattcctac cctgggctgc gcgtccgggc aaatgcatgg
     1801 ccggcttcca gggctctcag gtttaagtcc aggcttttgg gcgggacgct cttcccgacg
     1861 actcgctgta gggcggcgaa gcttcccggg aggaccttct cgatggccgc ccccagcatg
     1921 aacatgttgg ccacgatccg gttcttcaac tcttcctcgg cgaccttggt cgccggaact
     1981 ggaaaggtct tctggtaggg agcgggaatc gaccggatca gggtgctgtc gtagatcacc
     2041 agggtcttat ccgggtcccc ctcctggagg taccgtttta ccgattcctc ggacatgaag
     2101 accatgaagt ccggccgggc acttttggga aaatgaatct cctggtcgga aatgactacg
     2161 tcggttcggc acgcaccccc gcgggaaacc gggccgtagg attgggcctg ggtcacctgc
     2221 agctcgggaa attcttccat catctgggcc aggatcacgc ccattaggat gattccctgc
     2281 cctccgaatc cggcaaagac aatctccttc ctcatgattc ttttcccttc cctttcttct
     2341 tggccaccac ggtcagggcc aggtccgggc agttgatctc gcaaagcccg cagacccggc
     2401 aattttccat cttggccacc cggggcatgc gatagtcgag cgcgctccgt tcctgtcccg
     2461 cctcgaagac ttcgaatttg cagacgtact ggcagagcat gcaacccttg caatagcggc
     2521 ggtcgatctc gattcgttct gcgcccttca acgatctcct cctttggccc gggcttcttt
     2581 cgccttctga ttcatcttct gaacctcttc catgtattcc gggttctcga tcttaaggac
     2641 gtcccccagg gggattttcc cctgcccctc gacctctacc catttcgccg ggtcatcgac
     2701 cggcacgcag ttgtctttca accactgcag gttgaattgg ggagcgcctg acccatggat
     2761 gtaccttccc gtctgcaccg ggcactggga gatgacctcc acgaaagaaa agcccttgtg
     2821 ggtcaacatc tccttaaaag ttcggctgag cgccttagga tgggccgtgg agccgcggct
     2881 gacgaaggta gccccagccg caaccgcgac cttgcagccg tccaatggtt tttcgaggtt
     2941 gccgtagggg gtggtctggg tcctcgcccc cacctgcgtg ctcggagcga cctgcccgcc
     3001 ggtcatggcg tagatctgat tgttgaccac cacgacgccc atttcgatgt tccggcgcgc
     3061 cgcgtggagc aggtggaggc ccccgatggc caggccgtcg ccatcgccgg taaagaccac
     3121 gaccttgcgc gaaggccggg tggccttcag cccgatggcc gcgggaatgg cgcggccgtg
     3181 gaggaccttg aggacgtcca cgtgcatgta aacggggatc caggcgctgc agccgattcc
     3241 ggcggccagg gctaggtcgt cgatgattcc cagctcatcc accgcttcca gaaaggcctg
     3301 gacgatcatg ccgtcgccgc agccgggaca gaagataaaa ggaagaaaat tcttcctcag
     3361 gtatttttca ccgagtgggt tcatcgtctc tcctccagaa actgcaggaa ctcctgcggc
     3421 ggcgggtaaa tgcccccgat cttcgaaaac agctcgatct tcacctccgg ggcgcagatc
     3481 actctttgaa cctcgttgcg cagcatgccc aggttcatct ccgccacgat aatacgttta
     3541 gccccctcca gcctcttacg gaggagatcg tcggggaatg gccagatggt cttgacttcc
     3601 acggaccgga gggcgacgcc tttttcccgg gccatatcga cagcgctccg cgccgaccgg
     3661 ctgacggagc cgaacgagac gaaggcggtc tccgctttct cgtctccata ggtgcgaaca
     3721 tcagcgaatt ctgcgatgtg ggcctccacc ttgcggttga tgcggcggat gaacgattcc
     3781 gcttccttgg cattgccccg attgccggag agactcttgg cctgagagat caccgggatc
     3841 ttgtagcccg tgttgaaggc tggcatgggc gggacatcat cttctcccgc ttcgaagggg
     3901 acgaactttt ccatgggaac cgtgggcttc ttccgctcca caaactccaa ttggtcaggg
     3961 tccgggatga ccaccttttc ccggatatgg gcgacgacct cgtctgatag gatgatgacc
     4021 ggaatccgga aacgctcagc caggttgaaa gcccggatcg tgtggtcgaa cacctcctgg
     4081 gacgtggagg gacaaagagt gatcagggaa tagtccccgt tggacccgta gcgcgcctgg
     4141 taaagatccc cttgggccgt cgaggtagac tggcccgaag caggcccccc cctctgcacg
     4201 ttgacgatga cacacgggga ttccacgctg gcggccaggc cgattccctc ctgcatgagg
     4261 cagaagccgg gcccgctcgt ggcggtcatg gccttggccc cgccccagga ggctccgaca
     4321 acggccacga tggctgcgat ttcatcttcg gcctgcaggc aaaatccgtc caccagaggc
     4381 aggcgccggg ccatcccttc caggatctcg ctggccggtg tgatgggata gccggcgaag
     4441 aaccggcagc ccgcgaggag cgccgcctcc acgcaggcct cgtttccttg catgacctta
     4501 atgcagcctt ttttcatgac ttcctttctt ccttttcgcc tgattctttt tcaatcagac
     4561 cgggagtatc gtttcacccc cgcccaaaaa gtcaaggtct ttccttacat tggatggcgg
     4621 agtcgaatcc cgactacggt tacaggggac ggacaacagg taaagaacct tctgggaggc
     4681 gacagttgat ctctggcggt ccgacctcag ggtgatttta tcttatatcc tgtattttgc
     4741 tttatcttac cccatgcggc gataaagtaa agaccgaaaa aggccgactc tctaaacctt
     4801 gaactcatcc tgcagcagga ttgggatccg ccccggatgg gtcttcgcct tttccacata
     4861 cgttctaagg cttccccgga aaatcgggtc ggcacactgc tcgatgatcc gctcagcccg
     4921 ctcatagggt tcgagggccc tcaggtcagc aattccatgc tcggtgatca ggacatcgac
     4981 ttcgtgctcc gtatgatcca cgtgcgagac ccggggcacg atgctggaaa ccttcccctg
     5041 ttggatggtg gagggaaggg cgaaaatgga gatggaactg ttgcgggcga aatcatagga
     5101 gccggcgatt cccccgatga gctgggaccc cttcgagtgg ctcgaattga tttgcccctg
     5161 aatgtcggcc tccaggcccg aattgatcgc cagaacgccg aaccggctca gcatctccgc
     5221 cgagttggtc agggttacgg ggcggaagat aaaatagggt cggtaatgtt ccagacggga
     5281 aaaaaaccga ttcaaggccg aggcggaaaa acgcaggctg ctgcaggaaa cacctttcac
     5341 cttgcccgca tcgatcaaat ccaggagtgg gtcggtcacc actgcctgat aaaaaatgag
     5401 ggaagaaaga tcacttcgag ttaaagaaga aaggacaccg ttcagaacgt cgccgatccc
     5461 cacctccaga ggaggaagtt ccccgcgctg ctctctctcc cttcttagga atttccctat
     5521 attctcagcg atctgcggga ccctctcccc gggagggaac gccggcacgt cccgatcgtc
     5581 ccgatcggaa aagatgatgt gtttgatttt ctccgggtcc aacgagatgt aaggggagcc
     5641 aatcctttcc aggggatgct tcaaggggat gcaacggccc ggctctggat agtaaatgtc
     5701 gtgtaagccc tcaaccccca gaggacggcg caggttgatc tcgatgattg ccgaggaggc
     5761 caaatccaac cagtccggcg cgtcgtacac gcaggtggcg gggataatct ggcctttttc
     5821 attgatcccc gccgcttcta ccacggccac atcgattctc ccgtagtatc cccgccgggc
     5881 gtactggcac accttccccg ttttcccctc caagaatccc acttctcctc ggttgatagc
     5941 ctgccgcaat aatttcgacc ctacggtccc gatcctcttc cggatgcccc cgacttccgc
     6001 cagggcgtcc tcgatctccg gtccgagggg accggcacaa agtagatcaa tccgcaagga
     6061 atccccgttt ttaattctct ccctcagggc tccaaagact tcccttggat aaccggacag
     6121 agctgtgccg ctggttgcca cgaccatgcc atccctgaga aattgtgctg cctccttggc
     6181 cgtcgtgatc ttattgcgaa gttcggcttt tcgaattcgt ttttcccgtt cgatcaaagt
     6241 aatctcctct ctttatccgc gggatgcggc ccccattctt cgattttgac tccatggcat
     6301 tttttagccg gatctacgga tcggttcgat ctgcccgacg tccaggatga ggaaacggga
     6361 gggatccagg ttcctttgct ttacggttct cttcaagtcc agggccgggt aacccggggg
     6421 ctcatccccc agggcgaaag tgccccactg ggtggggatg aaatatttgg cgcccaagtc
     6481 ctgaaaagcg tcaagagcct ccggaacgct cttgtgggcg taatgcatga accagcgggg
     6541 gtggtaggcc gtcgtggaaa ggagggcgta atcgatattc ggaaattttc ttccgaactc
     6601 cttgtagccg ataaaatacc cgctgtcccc tccatagtaa atcgaggtct cggaggtcac
     6661 taacaggaag ctcgcccaga gcgtgcggtt atatccctgc ccgattcgtc tcgaccaatg
     6721 ctgtgccggt aggcaaacca gttttgcgcc tcctcccaca tccgcatctt cccaccagtc
     6781 catctccctt accgcaccct tgtggatcga ttccacgtat gccttcagtc cgagagggac
     6841 atagaatcgc gtattttccg gcagggaacg gatgctgtcg gcatcaagat ggtcgtaatg
     6901 attatgggaa atcaccacgt tcaatcgcgg cgtcaggtct ttcaattccc cgccggtcat
     6961 tgctggtgga gtcattcgtt tggggaggaa cgccctctcg gaaaagatcg ggtcggtgat
     7021 ccagaactcc cccttaaggc ggaggaggaa ggtgctgtga ccaacccagg caatgaaatc
     7081 ccctggaggc aaggattgga cccgcctttt taagtccggg atgaatttgg gtctgaaagc
     7141 cctctcttca tccgtgtact gtgctttctt ggagagttgc cacttcagaa cttgccaaaa
     7201 ctttccatgc tccatgggca tccaggggtt gaaatatttt ccaccgttga agtgggccgc
     7261 gtagagtttt tcaacgctct gatcctctac ccgctcccgc cacttcacct catcaaaggg
     7321 cactggatta tgcatacagc acgagaaaag taggataaga ctcacccacc caaaggtccc
     7381 cagctgcttc attca
//
LOCUS       DASPRR010000118         5792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_35679, whole genome shotgun sequence.
ACCESSION   DASPRR010000118 DASPRR010000000
VERSION     DASPRR010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35679"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VEL68_04200"
     CDS             complement(<1..418)
                     /locus_tag="VEL68_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35204.1"
                     /translation="MYQPTVSAGFLKSRPNEHESSGKAHSQIRNGFFVLVIDDLVKSL
                     EICFSVVPAKAGIQVLQINIRSLDSGFHRSDDFLRVYQSLEYPLSEFVPDFDIRISDL
                     SLSWDDIRSRKNRPYFRKVISPTPTTPSISRGFAGRR"
     gene            748..>5792
                     /locus_tag="VEL68_04205"
     CDS             748..>5792
                     /locus_tag="VEL68_04205"
                     /inference="COORDINATES: protein motif:HMM:NF039983.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HXZ35205.1"
                     /translation="MIRIGDLVEVPEIKTVIQLKDLERADLRRMILDSFVVTAEVSKS
                     LEMVLKSLAGPEGRGAFLKGNFGSGKSHFLGMLSLLLRHPESWASLVQQEASLEDFRQ
                     PLELKKFLVVEISLIQHRGTEFLEDIFLKEIFRELSRNLDRSFEGKESRRETFEEIRK
                     AMVRLGFSGLVLLVDELSEFLRSKADAHAYREDIRFLQYLGEEASSFPLWVVASLQEW
                     IEETGEISQDSFNKIKDRYPVRLTLGRAHIEELVSHRLIRQRQGAQEEIRKVFQSLRS
                     YFPSFPVDEKRFMKLYPVHPATITLLDRLKPLFSEHRGIVDFIHYRLKGDVERGIPTF
                     LDRPAHELLGPSAIFDHFLHRIREMAETQPYVEKGFEYYREEIPQVFKDPDQERVALE
                     AVKLLILFAISPVEARYTARHMAEMVLFRVTDLDGQTNYQYFRDILSRLVKETSFLVV
                     TPGKDPLDDRVSIDLRVDLSAILRRKVRQGVSEIFPGDRRLFQRLLPLAESTHIPFAG
                     WAEQGQQKVSTLWEYTRRSGILLLRQIDEVPLAEMENLAEEWVRMEEDFFLIVGTTHG
                     VERQYEHLRRSLLPALKDKCPGTFLFWVPASVGEEEEAAMKELLAVLLLQEKHREDSS
                     EGGPGAVEYLQNLLQGGKKRLGEILTRSYFQGHLLWDDRQVELSAYGFLTQERFLREF
                     IPPLLSRRFPKHHRVHPYLEALSPTSMPMLIKDFFATGSVEIDDRFKFGLRTILDSLL
                     KPMGLVKSKGNHYSLQVDPRTNELASHFFSLLDKGVQTPEALYWAFRKGDYGLLRVPF
                     EALVLALIFSGNIVPYQGQRKKGLEDVSRSGLQGITSLGKGEILSEEVRQLIPKHPLI
                     PEKFRKGTFTLPSQEALWGEVKGMKEPEAESLRNLLNRLGWASSFQAFKNLPWDSFRR
                     DVEDVLTQWEEVKVSFPAREGLERFLSAASREPFFPDKLRRIEELRNFFDRAERVLFV
                     QQYVGDPRLSIPDRPDYAGLRKEKEEILRFFEKGRVSIDGEKTEELMKRFQEFRERYI
                     HAYSEAHRRAKSGDQFAPYEKVRQSRRYQVLARLDQLEMISVPHNRSSADRSLAAVLQ
                     AECNGPSPESLQSNPRCSCGFSLGEEISFPPLREMEESIDKGIRESLEALASAPFQER
                     FIPYLNGLDAVGEKDKAAAIRRVLSITPGQEDILPRLEEALTPLAVREINEAFRGRVV
                     VVKRDLDQLYGALIQRKYTLSQVRKILREWLREEEVSDTTFVHFTGQGEAGGKTPEQD
                     AFLQFFQADFPHLLSLAQEAGPRVFKKILLLSLWIDGYGIPPAEMIHLFPLLEKGKAE
                     RGDLLVRELSRAARSLHQKNPSLFERLLQEMEGEEGIAREAWKLLEGRPAQEIFRRET
                     LLPSILREALERLFASPEEREGEDFSHLENPLTPLRTPDFIRRQAEMAGALQDMSVLR
                     QKVQTLRRRENSPPQDFAKWESLYTKQLSPLAYVLGTVGQRIQRMDVPLPPSLKERLA
                     QAETICSSLSRSFADYYRRSLPQWEAGEEKRPIMVEDLPALNPWKRKLAEGREKVFIL
                     LDGMRLDLWEYVKESFFKPLAHHLRIVNEGTLWAHLPTSTPRQTELLQQAWEKFIPGG
                     KEWTEFLWKIGGIDERVHTERGGLEYLFRNVLQYLQLDLAPQLRELPPQTLLLFFSDH
                     GFVENPHFDKTDK"
BASE COUNT         1270 a         1674 c         1636 g         1212 t
ORIGIN      
        1 ctcgccgccc ggcaaacccc cgggagatgc tgggcgtggt gggagtgggg gagattacct
       61 tccggaaata tggtcgattt tttctcgatc tgatatcatc ccatgacaaa gacaaatccg
      121 aaatccgaat atcgaaatcc ggaacaaatt cagaaagcgg gtattcaagt gattgataca
      181 ctcgtaaaaa gtcgtcactc cggtggaaac cggagtccag agatctaata tttatctgaa
      241 gtacctggat tccggctttc gccggaacga cagaaaaaca tatttcgaga ctttttacga
      301 gatcatcaat gaccaaaaca aagaatccgt ttcgaatttg agaatgggct tttccacttg
      361 actcatgttc attcggccta cttttcaaaa atccggcgga tacggttggc tgatacaaag
      421 aacaatcttc cgcttggccc ttgaggctct tcaggttgat tctccctccc cctcgatggg
      481 gagggaaggg tggggtgaaa agtcttcgga atctcgtgct tctccgcttc aatccccctc
      541 acctcgaccc tctcccacga agtggagagg gggtttttgg gctggaaatg tcaaaatgag
      601 ttaaatggaa agcccatttg agaaatccgg aattgtttcg agattcggat ttcgtgcttc
      661 gaatttttag aggatggatt tattttaggg aatcccgttt ccattttgca gacgttctga
      721 cctttcagag tcccaggagg tagggaattg attcggattg gagacctcgt cgaggttcct
      781 gagatcaaga cggtcattca gctgaaggac ctggagaggg cagacctcag gcggatgatt
      841 ctcgactctt tcgtggtcac cgccgaggta tcgaagagcc tggagatggt tctgaaatct
      901 ctggccgggc cggaagggcg gggggccttt ctaaagggaa atttcgggtc gggtaaatct
      961 cacttcctgg gcatgctcag tcttctgtta cgccaccccg aatcctgggc ttcccttgtc
     1021 cagcaggagg cgtccctgga ggatttccgg cagcccctcg aactgaagaa gttcctcgtc
     1081 gtggaaatct ccctcatcca gcaccggggc acggagtttc tcgaagacat ctttctaaaa
     1141 gaaattttcc gcgaactctc gagaaacctg gaccgatcct tcgagggaaa ggaatcgcgg
     1201 agggaaacct ttgaagagat tcggaaggcc atggtaaggc tgggtttctc cggcctggtc
     1261 cttctggtgg acgaactctc cgagtttctt cgctccaagg ccgacgccca cgcctaccgg
     1321 gaggatatcc ggtttctcca gtacctggga gaggaggcct cctctttccc cctctgggtc
     1381 gtggcctcgc tgcaggaatg gatcgaggag acgggcgaga tcagccagga ttccttcaat
     1441 aaaatcaaag atcgctaccc ggttcgcctg accctgggcc gggcccacat cgaggagctg
     1501 gtttcccacc gcctaatccg ccaacgacag ggcgcccagg aggaaatccg aaaagttttt
     1561 caatccctgc gaagttactt cccctccttc ccggtcgacg agaaaagatt catgaagctc
     1621 tacccggtcc atcccgccac catcaccctg ctggaccgac tcaagcccct cttttcggaa
     1681 catcggggga tcgtggactt catccattac cggctcaagg gagatgtgga acggggaatc
     1741 cccaccttcc tcgaccggcc ggcccacgaa ctcctggggc cctccgccat cttcgaccat
     1801 ttcctccacc gcattcggga gatggccgaa acccagcctt acgtggagaa aggattcgaa
     1861 tattaccggg aggagatccc ccaagtcttc aaggacccgg accaggagag agtggccctg
     1921 gaagcggtca agcttctcat ccttttcgct atctccccgg tggaagccag gtacacggca
     1981 aggcacatgg ccgagatggt cctcttccgg gtgacggacc tggatggaca gaccaactac
     2041 cagtacttcc gggatatcct ttcccggctg gtgaaggaga cctccttcct ggtggtgact
     2101 ccaggaaaag accccctcga cgaccgggtc tccatcgacc tgcgggtgga cctttccgcc
     2161 attttgcggc gcaaggtccg gcagggggtc tcggaaattt ttcccggaga ccgccgccta
     2221 ttccagcgtc ttcttcccct ggcggagtct acccatatac cgtttgccgg ctgggcggag
     2281 cagggacagc agaaggtctc caccctttgg gaatataccc ggaggagcgg aattctcctc
     2341 ctccggcaga tcgacgaggt cccgctcgct gaaatggaaa acctggcaga ggaatgggtg
     2401 cggatggaag aggatttctt cctcatcgtg gggaccaccc atggggtgga gcggcagtac
     2461 gaacacctgc gccggagcct gcttcccgcg ctgaaggata aatgcccggg cacttttctc
     2521 ttctgggtcc ccgcttccgt aggggaggaa gaggaagcgg cgatgaagga actcctggct
     2581 gtgctccttc tgcaggagaa acaccgggaa gattcctccg aaggggggcc gggggcggtc
     2641 gaatacctcc agaacctatt gcagggaggt aagaagaggc tgggggagat tctgacccgc
     2701 tcttatttcc agggccacct cctctgggat gaccggcaag tggagctttc ggcgtacgga
     2761 ttcctgaccc aggaaagatt cttgcgggaa ttcatccctc ccctcctttc gagaagattc
     2821 cccaaacacc accgggtgca cccctacctg gaagccctgt ccccgacttc gatgcctatg
     2881 ctcatcaagg atttcttcgc cacgggaagc gtggagatcg acgaccggtt caaattcggg
     2941 cttcgtacga ttctggacag cctgctgaag cccatgggat tggtgaagtc caaggggaac
     3001 cactattccc tccaggtgga cccgagaacc aatgaactgg cgtctcactt cttctccctg
     3061 ctggataagg gtgtccagac gccggaagcc ctctactggg cctttcgcaa gggagactac
     3121 ggcctgctgc gggttccctt cgaagccctg gtcctggccc tgattttttc agggaacatc
     3181 gttccctacc aggggcagag gaagaagggg ctggaggatg tctcccgcag cggccttcag
     3241 ggcatcacca gtctggggaa aggcgaaatc ctctccgaag aagtccggca gctcatcccc
     3301 aagcacccgc tcatcccgga aaaatttcgc aaggggacct tcacattgcc ttcccaggag
     3361 gcgctctggg gtgaagtgaa aggaatgaaa gaacctgaag ccgaatccct ccgcaacctc
     3421 ctcaaccgtt tgggatgggc ctcttccttc caggccttca aaaaccttcc ctgggattcc
     3481 ttccggcggg atgtggagga tgttctgacc cagtgggagg aagtaaaggt tagcttcccc
     3541 gcccgggagg ggttggagcg gttcctttca gcggcttcga gggagccgtt ttttcccgac
     3601 aagctccggc gaatcgagga attgaggaat ttcttcgacc gtgcagaaag ggttctcttc
     3661 gtccagcagt atgtcggcga cccgcgcctg tccattcccg accgccccga ttatgcgggc
     3721 ctccggaagg aaaaagaaga aatcctccgg ttcttcgaaa aaggccgggt ttccatcgac
     3781 ggggagaaaa ccgaggagct gatgaagagg tttcaggaat tccgggagag atatatccat
     3841 gcctacagtg aggcccaccg ccgggccaag tcgggagacc agttcgcccc ctatgaaaag
     3901 gtacgccagt cgagacgcta ccaggttctg gcccggctgg atcagctgga gatgatctcg
     3961 gtcccccaca accggagttc tgcggaccgt tcgctggccg cggtcctcca ggcggagtgc
     4021 aacggcccct cgccggaatc gcttcagagc aatccccgat gctcctgcgg gttttccctg
     4081 ggagaagaaa tatccttccc gccccttcgg gaaatggaag agtctatcga caagggcatc
     4141 cgggagagcc tcgaagccct ggcctctgcc cccttccagg agagattcat tccctatctt
     4201 aacgggctcg acgccgtagg ggagaaggac aaggcggcgg ccatccggcg ggtcctttcc
     4261 atcaccccgg gccaggaaga tatcctcccc cgcctcgaag aggctttgac ccccctcgcc
     4321 gttcgggaga tcaacgaggc tttccgggga agggtggtgg tcgtaaagag ggacctggac
     4381 cagctctacg gggcgctgat ccagcgtaaa tacaccctgt cccaggtccg caagattctc
     4441 cgcgagtggc tgcgggaaga ggaagtctcg gatacgacct tcgtccattt caccggccag
     4501 ggggaggccg ggggcaaaac ccccgagcaa gatgccttcc tccaattttt ccaggccgat
     4561 tttccccacc tcctttctct ggcccaggag gccggaccca gggtcttcaa aaagatcctt
     4621 cttctctccc tttggatcga cgggtatggc atccctccgg cggagatgat tcatctcttt
     4681 cccctattgg aaaagggaaa agcggagagg ggagacctcc tagtccggga gctttcccgg
     4741 gccgcacgat ccctccacca aaaaaacccg tctctctttg aacgcctcct ccaagaaatg
     4801 gagggagaag aagggattgc ccgcgaagcc tggaagcttc tggaaggaag gcccgctcag
     4861 gaaatctttc gccgcgaaac cctccttcct tccatcctgc gagaggcttt ggaacggctt
     4921 ttcgcctcgc cggaggaaag ggaaggggag gatttttccc acctggagaa ccccctgacc
     4981 cccctgcgga ccccggattt catccgcagg caggcggaga tggcaggggc ccttcaggac
     5041 atgagcgtcc ttcggcagaa ggtccagact ctgagaagaa gggagaacag ccccccgcaa
     5101 gactttgcca agtgggagtc tctctacacc aagcaactaa gtcccttggc ctatgttttg
     5161 ggaaccgtgg gccagaggat tcagcggatg gatgtgcccc taccccccag cctaaaagaa
     5221 cggctcgccc aggcggaaac catttgcagt tccctttccc ggagctttgc cgactattac
     5281 cggcgttccc tgccgcaatg ggaggccgga gaggaaaaga gaccgatcat ggtcgaggac
     5341 ctgccggccc tgaatccctg gaagagaaaa ctcgctgagg gaagagaaaa agttttcatc
     5401 ctcctggacg ggatgcgctt ggacctgtgg gaatacgtaa aggagagctt cttcaaaccc
     5461 ctggcgcacc atttgaggat tgtgaacgaa ggaactcttt gggctcatct tcccacttcc
     5521 acccccaggc agacggagct tctgcagcag gcctgggaaa aattcattcc gggtggaaaa
     5581 gagtggacgg aattcctttg gaagatcggg ggtatcgatg aacgggtaca cacggagcgg
     5641 gggggattgg aatacctctt ccgcaacgtc cttcagtacc tccagctcga cctggccccg
     5701 cagctccggg agctgccccc gcaaaccctc cttctctttt tctccgacca tggattcgtc
     5761 gaaaaccccc acttcgacaa gaccgacaag ta
//
LOCUS       DASPRR010000119         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_35919, whole genome shotgun sequence.
ACCESSION   DASPRR010000119 DASPRR010000000
VERSION     DASPRR010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35919"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VEL68_04210"
     CDS             complement(<1..329)
                     /locus_tag="VEL68_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ35206.1"
                     /translation="MNKPILLTTPLSAEEVRKLKVNDIVTLTGTIYTARDMAHLKLRE
                     LKESGKPLPEALEGSVIFHAGPVVKKTDRGYDLLNIGPTTSIRMEPHSDFLGRLGVRA
                     IVGKGGLG"
     gene            complement(326..1207)
                     /locus_tag="VEL68_04215"
     CDS             complement(326..1207)
                     /locus_tag="VEL68_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXZ35207.1"
                     /translation="MKALEKKFADAFYESLVRGVTSISPDILRMLKEARNDESPQGRS
                     MLEALIQNAELAQKMKRGVCQSPGIPCIYIRSKPELAGINIRKIAGEAIVQATKEGYL
                     RPSIVHPLTRKNTGNNSGPGIPNFEVEVDSALEYLEVIVSFKGCGPELFNAFKTFTPE
                     SLGKNLEGLKRFLLESVINAGGKPCPPIGLGVGIGGQMDGAAKLSRMAISTRKWNDRN
                     PDPELDRMEQELKARINQLGIGPGGVGGKTTALAVKIGLAYTHTAICPVAVNFHCWVG
                     RRFGFRFFADGKLEEIL"
     gene            1438..2127
                     /locus_tag="VEL68_04220"
     CDS             1438..2127
                     /locus_tag="VEL68_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HXZ35208.1"
                     /translation="MNDLDFERVMVVFAHPDDAEVQCAGTIALWVEAGKKITYVVLTK
                     GDKGTPDPSMAGEVLAKVRQEEQLKAASELGVEKVLFLPNSDGELEVNLERRKKLTYI
                     IRERQPEVLITHDPWMRYQLHPDHRASGTLALDAVISARDPLYFAEQIQGGLTPCRVK
                     RALLFASDQPDFWVDIGGTIERKISALGHHRSQVMRWPNWEEWMRKRAHDAGASQGLK
                     YAEAFKRLILR"
     gene            complement(2215..3138)
                     /locus_tag="VEL68_04225"
     CDS             complement(2215..3138)
                     /locus_tag="VEL68_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXZ35209.1"
                     /translation="MKKSLILMTVGILVVAFLGTSAFAQQKMVLKKYPEIKFGFTTTN
                     FLKPLPVSLDNKKKLVDLAAELGCSWVEIRDPSGALSPEECKQLMAYAKSKNLELGYA
                     LQIGLLDPAYWEIFSRGLANAPLFQGPGTIRTLAAGPEFDRDPKKTTWTLSELHKTVK
                     VANQAANLARAAGLKYVVENSFPALKGDGITGFGMTEFAANVNSNLFIQADVANFFAV
                     SRVVPKPEEVKAFLEKYASRIPYIHVKASSSEHKVLPVLAQNELDLDTVFSIMTKNKI
                     RYAAVELTQPDTFEECANNLKKSFEYLKNNY"
BASE COUNT          685 a          905 c          910 g          735 t
ORIGIN      
        1 cccagccccc ctttgcccac gatggctctt acgcccaggc ggcccaagaa gtcggaatgc
       61 ggctccatac ggatgctggt ggtcgggccg atgttaagca ggtcataccc gcgatctgtt
      121 ttcttgacca ccgggccggc gtggaagatg accgatccct ccagggcttc tggcaagggt
      181 ttgccgcttt ctttcagctc cctgagcttc aggtgggcca tgtcccgggc ggtataaatg
      241 gtcccggtca gggttacgat gtcattgacc tttagtttcc gaacctcctc cgcagagagc
      301 ggggtagtta gaagaatggg cttgttcata ggatttcctc caatttcccg tcagcaaaaa
      361 accggaaacc aaaacggcgg ccgacccagc agtggaagtt gacagccacc ggacagatgg
      421 ccgtatgggt atacgcaagg ccgattttta ccgccagggc cgtcgtcttg ccgccgaccc
      481 ctcccgggcc gatccccagt tgattaatcc ttgctttgag ttcctgctcc atccggtcca
      541 gctcggggtc gggattccgg tcgttccatt ttctggtgct gatggccatg cggctgagct
      601 tggccgcgcc gtccatctgc cccccgatgc ctactcccaa gccgatgggc ggacagggct
      661 ttccccccgc attgatgacc gactccagga ggaaccgctt caacccctcc agatttttgc
      721 ccagggattc aggggtaaag gtcttgaagg cattgaacag ctccggcccg catcccttga
      781 aactcacgat gacctccagg tactccaggg cagaatctac ctccacctca aagttgggga
      841 ttccggggcc cgaattattc cccgtgtttt tccgggtcag aggatgaaca atgctgggcc
      901 gaagatagcc ttctttcgtc gcctggacga tggcttctcc ggcgatcttt cggatgttga
      961 tgccggccag ttccggcttg gaccggatgt agatgcaggg aatcccgggg gactgacaca
     1021 cccccctttt catcttctga gccagctccg cattctggat caaggcctcc agcatggacc
     1081 ttccctgggg ggactcgtcg ttcctcgctt ccttcaacat ccggaggatg tccggagaga
     1141 tactggtaac gccccgaacc agagactcat aaaaggcatc ggcgaatttt ttttccaaag
     1201 ctttcatgac cgctcctatt attgggtggg tggccgcaga atcctcagca gccctaattt
     1261 ccgtttaaca cagaatcgcg aagcggtcaa tttctttacc ggaatttttt atccaggttt
     1321 caatgagcgg taagggttct tccgggccgc cagtaggcca ttgaatttgc gcatgaaatg
     1381 ggttagaaag aatagattcg atcctaaacc ctggacaata tttttaagga gttctcagtg
     1441 aacgatctgg atttcgagcg ggtgatggtc gtctttgccc atcccgatga cgcggaggtg
     1501 cagtgcgccg ggaccatcgc cctgtgggtt gaagcaggga aaaagatcac ctatgtggtt
     1561 ctgactaaag gagacaaagg cacgccggac ccttccatgg ccggggaggt gctggcaaag
     1621 gtgcggcagg aagaacagct caaggccgcc agcgagctgg gggtcgaaaa ggtcctcttc
     1681 cttccaaaca gcgacgggga gctggaggta aacctggaga ggcgaaaaaa attaacctac
     1741 atcatccggg agcgccagcc ggaagtcctg atcactcatg acccctggat gcggtatcaa
     1801 ctccatccgg accatcgcgc ctccggaacc cttgccctcg atgcggtcat atcggcccga
     1861 gaccctcttt attttgcgga gcaaatccaa ggcgggctga cgccctgccg ggtcaaaagg
     1921 gccctgctct tcgcctccga ccagcccgat ttctgggtgg acatcggggg aacgatcgaa
     1981 agaaagatat ccgctctggg ccatcaccgg agccaggtga tgcggtggcc gaattgggaa
     2041 gagtggatgc gcaagcgggc gcacgacgcc ggcgcttccc aaggcctgaa atacgccgag
     2101 gctttcaagc ggttgattct gcggtagaat caaaaagggt gcatcccccc gtcctccatt
     2161 ttcgggggcg gggggttttc agatggggaa gcggcggata taccgccgga aagatcaata
     2221 gttgttcttc aagtattcga agctcttctt cagattgttc gcacattctt caaaggtgtc
     2281 cggctgggtc agctccacgg ccgcataccg aattttattc ttggtcatga tcgagaagac
     2341 cgtatccaaa tccagctcgt tctgcgccag gaccggcagg accttgtgct cgctggagga
     2401 tgccttaacg tgaatatagg ggatccggct ggcatatttt tccaagaacg ccttcacttc
     2461 ttccggcttg gggacgaccc gggagacggc aaagaagttc gccacgtcag cctggatgaa
     2521 caggttggag ttcacgttgg cggcgaactc ggtcatcccg aacccggtaa tcccgtcccc
     2581 tttcagagct gggaaggaat tctccaccac gtattttagc cccgctgcgc gggccaggtt
     2641 ggccgcttga ttggccactt tgacggtttt gtgcagctct gaaagagtcc aggtggtctt
     2701 tttggggtct ctatcgaact ccgggccggc ggccagggta cgaatcgtcc cgggtccctg
     2761 gaagaggggc gcattggcca atccccggct aaaaatctcc caataggcgg ggtccaagag
     2821 gcctatctgc aaggcatagc cgagttcaag gttcttggat ttggcatagg ccatgagctg
     2881 tttgcactcc tccggtgata gggcaccact ggggtcgcga atctcgaccc aagaacagcc
     2941 cagttcggcg gcgaggtcca ccagcttttt cttgttgtcc aaggaaacgg gaaggggttt
     3001 caagaagttc gtggtggtaa atccgaactt gatctccgga tatttcttta agaccatttt
     3061 ttgctgggcg aaggcactcg tacccaggaa agcaacgaca agaattccca ccgtcataag
     3121 aatcagactc ttcttcataa tgactcctcc tttcagggtt aaagggtgct gcgaatcctt
     3181 cgttcccttt ccttcgaagg taagcttagc acctaaatca caaaccgaga ccgga
//
LOCUS       DASPRR010000120         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1155043, whole genome shotgun sequence.
ACCESSION   DASPRR010000120 DASPRR010000000
VERSION     DASPRR010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155043"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VEL68_04230"
     CDS             complement(<1..879)
                     /locus_tag="VEL68_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35210.1"
                     /translation="MEEPDSQLGVVSYGLYLPAGSESAEEIARRSGLSRNQVIEELGL
                     ERKCLPSAEDLPVVMAVKAARQALERAREVDPEEIDVVIWTGEEYKDYICQTAGIRVQ
                     EEVGARKAWAFDLIGQGTTSLVGLRTAQDLMIGDPSVRTVLLAGGTRNIDLVDSSNPN
                     ARWMLPASASGAALLLRRRHPRNRLLGTAFCVDPEMADEVYVPGGGTVWPFSPEILNT
                     EAMFFQVARPQEVETYLAGRLAPQLVSLIKKTMAQAGFSGQNPEYLALRHLRPQERAR
                     VFKGLRLRREATDALAD"
     gene            complement(951..>2079)
                     /locus_tag="VEL68_04235"
     CDS             complement(951..>2079)
                     /locus_tag="VEL68_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ35211.1"
                     /translation="NEAVGRSLASLWEGLPSDAPPDAVLRPSDPQLIIYTSGTTGVPK
                     GVILTYGMITWNSINTNLGWGLRPEDRTILHSSLFYTAGWNVFTLPLVHLRATNVLVK
                     SFDADLILDLIERERVTVFFGVPTMFQMLLESPKFAKTDFSSIRFLVSGGAPLPKKLI
                     EIYQTQKGIRLWEGFGLTEVGPNNFMANGKPGTVGNPMPHVDVRVVDSQGREVPPGQD
                     GELLLRGDHMCSGYWKKPEATAEAIRSGWFHTGDLARIDEDGHISIVGRKKDMIISGG
                     INIYPAEVEKAIEGHPRVAAAAVIGVADEKWGEVGKAVLELKPGERLTLKELQGFLSE
                     RLGKYKVPKYAVEVKELPRTAASGKVQKFILKEKHGKPDNE"
BASE COUNT          381 a          651 c          596 g          451 t
ORIGIN      
        1 gtccgccaga gcatcggttg cttctctccg gagtctcaat cccttgaaaa ccctcgcccg
       61 ctcctgcggg cggagatgtc gaagcgccag atattccggg ttttgcccgg agaatcccgc
      121 ttgagccatg gtctttttga tcaaactcac cagctgcggg gccaatcggc cagccaggta
      181 tgtttccacc tcttgcgggc gggcaacctg gaagaacatc gcctcggtgt ttaagatttc
      241 cgggctgaat ggccagacgg ttccgcctcc gggcacatac acctcgtcgg ccatctccgg
      301 gtccacgcag aaggccgttc ccaaaagtcg gttccgggga tgccggcgcc ggaggagaag
      361 ggcagcgccg ctggcggaag caggaagcat ccaccgggcg ttggggttgg aggaatcgac
      421 caggtcgatg ttccgagtcc ctccagcgag aaggacggtg cgaaccgaag ggtccccgat
      481 cattaggtcc tgcgctgtcc gcagcccgac cagggaggtg gttccctggc cgatcaaatc
      541 gaaggcccat gccttccgag cgcccacttc ctcctggacc cggatgccgg cggtctggca
      601 gatgtagtcc ttgtattctt cccctgtcca gatgactaca tcgatttcct ccggatcgac
      661 ctccctggcc cgctccaggg cctggcgggc ggccttcacc gccatgacca ccggcaggtc
      721 ttcggcagag gggaggcatt ttctttccaa tcccagttct tcgatcacct ggttccggga
      781 aagcccgctc cgcctggcaa tctcctccgc actctcagag ccagcgggaa ggtacaatcc
      841 ataacttacc acgcccagct gggaatcagg ctcctccatg caaaatccac tgctcctctc
      901 gcgatagggg tttaaaacct gaaacccggg acctaagacc tgaaaccgtt ttattcatta
      961 tccggtttcc cgtgcttctc cttgagaata aacttctgga ccttgcccga ggcagcggtc
     1021 cggggcaact ccttcacctc gaccgcgtat ttggggacct tgtattttcc caggcgttcg
     1081 ctcaagaaac cctgcaactc cttgagggtc aggcgttccc ccggttttag ttccagtacg
     1141 gccttgccca cttctcccca cttctcatcg gccaccccga tgaccgcggc cgcagccact
     1201 cgggggtgac cttcgattgc cttctcgacc tccgccgggt aaatgttgat ccccccggag
     1261 atgatcatgt ctttttttcg ccctacgatc gatatgtggc cgtcttcgtc gatccgggca
     1321 aggtctcccg tgtggaacca tccgctccgg atggcctccg ccgttgcctc cggctttttc
     1381 cagtagccgc tgcacatgtg gtctccgcgg agaagcagct ccccgtcctg gcccgggggc
     1441 acttccctcc cctgcgaatc cactactctt acgtccacat ggggcatggg gtttcccacg
     1501 gtcccgggtt tgccattggc catgaagtta ttggggccca cctcggtgag tccgaaacct
     1561 tcccacagcc ggattccctt ctgggtttga tagatttcga tgagtttttt cggcagaggg
     1621 gctcctcccg agaccaagaa gcggatggag gaaaagtcgg tcttggcaaa tttgggtgat
     1681 tcaaggagca tctggaacat ggtgggaacc ccgaaaaaga cggtcacccg ttcccgctcg
     1741 atgaggtcca ggatcagatc ggcgtcgaag cttttgacta ggacgttcgt cgcccggagg
     1801 tgaacgaggg gaagggtgaa cacgttccag cccgcggtgt agaaaaggga ggaatgaaga
     1861 atcgtccggt cttccggccg gagtccccag cctaggttgg tattaatgga gttccaggtg
     1921 atcattccgt aggtcaggat aacccctttg gggaccccgg tggtcccgga agtatagata
     1981 atcagctggg ggtccgatgg tctgaggacc gcatccggcg gggcgtccga gggcagtcct
     2041 tcccagaggg aagccaggct ccggcccacc gcctcgttc
//
LOCUS       DASPRR010000121         9645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_36479, whole genome shotgun sequence.
ACCESSION   DASPRR010000121 DASPRR010000000
VERSION     DASPRR010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36479"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VEL68_04240"
     CDS             complement(<1..335)
                     /locus_tag="VEL68_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXZ35212.1"
                     /translation="MTIFRTHILIPVDQNTVQAGVFEVKRHLENQLQTRKLDQEIKVL
                     ETGTLGVVGQGVMLVVFPERICYTRVKVEDIPRIVEEHLVKGRVVRNLEHFTLDIKPG
                     VREPYQLGLA"
     gene            complement(332..790)
                     /locus_tag="VEL68_04245"
     CDS             complement(332..790)
                     /locus_tag="VEL68_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXZ35213.1"
                     /translation="MTPQKIIQQYDPAPENLLSILHDLQDANERHYLTEQDLQLAAGH
                     LRLPFSFVQGVATFYTMFSLTPRGKFIIRVCESPPCHLLGSTTIAQELMKLLGIQFGE
                     TTRDDLFTLEMSSCLGVCGVAPALMVNDEVYGNLTPQRIREILEEKRRGA"
     gene            complement(810..3881)
                     /locus_tag="VEL68_04250"
     CDS             complement(810..3881)
                     /locus_tag="VEL68_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ35214.1"
                     /translation="MRPRLDILVCGGGACISSHSREFKNKLSEEINQKGLTEEVNLIE
                     TGCMGPCQLGPVMVVYPDGSFYINLKEEHAEEIVLEHFLKGRPVQSLLWTTPEARKIV
                     EEQKQVPFFEKQKKIVLALCGRIDPENIEEYIAQRGYEALGTILTTMKPEEVIDIMTK
                     SGLRGRGGAGFPTGLKWKFARANPAPQKFVICNADEGDPGAFMDRAVCEGDPHAVLEG
                     MAIAGYAIGASRGYIYVRAEYPLAIDRLNIAIQQARQMGLLGKDIFETGFEFDLELRM
                     GAGAFVCGEETALIASIEGQRGLPRPKPPFPASKGLWQQPTIINNVETLANIRHIFLN
                     GWEWFASIGTATSKGTKVFALSGKVNNTGLVEVPMGTTLGELVFDIGGGIPDDKAFKA
                     VQTGGPSGGCIPPKYLNSAIDYESLKQIGSIMGSGGIIVLDEDSCMVNMAMYFLDFTM
                     EESCGQCVPCRVGLKQMRDLLEKITLGLGTLEDLEKLEKLAHWVKNTSLCGLGQTAPN
                     PVISTLQYFRHEYLEHIQDKACRAGVCAALFYAPCENACPANVDAASYVSYMAEGRLQ
                     EAYLRHMENNPFPIACSRVCPAFCEKKCSRGKYDQAIAIREIKRLFADWAVEQDIGFL
                     PPKSPKKERVAIVGAGPAGLACGFYLTRMGYKPVVFEAMPVPGGMMKLGIPDFRLPKE
                     KLAAEIGVIERAGVEIRLNSPVKSVEDLQKQGFQAVFLGTGAWTPQPLSVPGIDLKGV
                     LSGIDFLREVNLGKKVNVGQRVVVIGGGSTAMDAARVSKRLGAESVQIVYRRTRAEMP
                     AWPEEIEEADEERIQMDFLVNPVKVEGKNGKVTGLTCIRTELGEFDTSGRRKPVPIQG
                     SEFTIPVDTIILCLGQKLSGGLNGAKLKLDKRGQVEVNARTLATSLPGVYAGGDSVNP
                     STIIESVAQGRKAAMEIDRHFGYEGKLFARERKPVAVSYDEEAYLKNIPRKTPRLEEV
                     EKRIRTLALEVNLGFLREEVMEEARRCMHCDRNQTAVAAGEETDIGHAEAV"
     gene            complement(3896..4258)
                     /locus_tag="VEL68_04255"
     CDS             complement(3896..4258)
                     /locus_tag="VEL68_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXZ35215.1"
                     /translation="MNLEDLKKLREKREKDMVLRDGKARVKVAVSMGTSGIAAGARQT
                     MKAFLEEIERRGLKDVIVTQSGERGLSSKEPIVNVTVEGKPSVVYGDVSADIARKIVA
                     EHLVNGNPVSDYVIETQK"
     gene            complement(4279..4782)
                     /locus_tag="VEL68_04260"
     CDS             complement(4279..4782)
                     /locus_tag="VEL68_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXZ35216.1"
                     /translation="METQQVQQPDSVRYGELQQFIDANRQIAGPLIQVLHRAQKIFGY
                     LPEDVQKYVARELRLPLSKVHGVVTFYNFFRTEPMGDHLINVCLGTACHVKGASEIIK
                     SLEKSLAIKMGGTTPDRFFTLSSARCFGACGLAPCMMIDNEVYGRLNPTKANQIIQEI
                     KSNIHTA"
     gene            complement(5339..6106)
                     /locus_tag="VEL68_04265"
     CDS             complement(5339..6106)
                     /locus_tag="VEL68_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXZ35217.1"
                     /translation="MLKEYHADLHIHTCLSPCASLDLSPRRIVERALEEGLDLIAITD
                     HNIAKNVPAVMHQAEKFGLKIIPGMEVQSREEIHILTLFPDWPSLSAWEAVVLEHLPG
                     IPNDPEFFGDQPIVDEEGNILEFEERLLINSIDYSLNEIRGQVLQRGGLFIPAHFDKD
                     SFSLISQLGFVPFDLEPEALEMGRKNQAAQAANPRDPSARVPRVVCSDTHQVDDIGRA
                     RTVFLLAEPTLGELRLAFQGKEGRKVLKLIFPGSPQG"
     gene            complement(6260..7717)
                     /locus_tag="VEL68_04270"
     CDS             complement(6260..7717)
                     /locus_tag="VEL68_04270"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXZ35218.1"
                     /translation="MKVVIPIINWSVIGPELVLSLTAMVLLLLNVLAKKWAKGAIPIL
                     SLIGVAISLVLCFSLWGQSQYAFNRMVVLDNYSLFLKAIFLITAGLTILMSIRFLRAE
                     GFDYGEYYILLLFAAVGMMFMASAADLIIVFLGLETFSLAVYALAGFFRTNPKSNESS
                     LKYFLLGAFSAGFLLYGIALIYGATGTTNLKGIFDFLRRADLFTDPLLLIGMGLLIVG
                     FGFKVASVPFHMWTPDVYEGAPTAVTAFMSVGPKAAGFAAFLRVFLYSLSSLQTEWVW
                     VLWVLAVLTMTLGNIVAIAQKNIKRMLAYSSIAHAGYILVAMVASSEMSTASILYYLL
                     AYAFMNLGAFGVVILYGRKGEENILVSDYSGMASRYPLLAAAMAVFMFSLAGIPPTAG
                     FVGKFYIFSAAVKAGYVGLAIIGVLNSALSVYFYLRVTVMMYMRGPEKEAAGGLDFSP
                     GMVIALLIAVFFTLQMGIFPANYLNLARQSVLALM"
     gene            complement(8004..9566)
                     /locus_tag="VEL68_04275"
     CDS             complement(8004..9566)
                     /locus_tag="VEL68_04275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXZ35219.1"
                     /translation="MISLAGIPLLTLITFFPMVGVLILLFLDKEKKDTIRQVTLGVSL
                     LEFLFSLPLFFNFRLDTAAMQFVEKASWIPEYGITYYLGIDGISLFLVLLTTFLTFVS
                     IMACWADIQDKVKEFMICILFLETGMIGVFVSLDLFLFYVFWEVMLIPMYLLIGVWGN
                     PKRRIYAAVKFFIYTMVGSVLMLVAILVLYFHNGAATGKYTFDLLQLYNLQVSRDIQV
                     WLFLAFGLAFAIKVPMFPFHTWLPDAHTEAPTVGSVLLAAVLLKMGTYGFLRYSIPLF
                     PQASFQLIPLVSVLALIGIVYGALVCLVQKDLKRLIAFSSVSHLGFVMLGLFAFNIQG
                     IQGGILQMLNHGFSTGALFLLVGMIYERRHTRMIEDFGGLWKQMPIFAAFFMVVTLSS
                     IGLPGLNGFVGEFLILVGTFKANGPYAVVAATGVILAAAYMLWMFQRVMFGELTKPEN
                     KVLKDLSKREVLVLSCVTLFIFWIGVYPQTFLSRMEPTVKAYLVQVQQKYQAGVQMQE
                     GEKIKVAQAEKK"
BASE COUNT         2073 a         2794 c         2762 g         2016 t
ORIGIN      
        1 gccagcccca gctggtaagg ctcccggact ccgggtttga tatccagggt gaagtgttcc
       61 aggtttcgca cgacccgccc tttgaccagg tgctcctcca cgatgcgggg gatatcctcg
      121 accttcaccc gggtgtagca aatcctttcg ggaaaaacga ccagcatgac tccctggcct
      181 acaactccga gggtacccgt ctccaggacc ttgatttcct ggtcgagttt gcgagtctgc
      241 agctggttct ccaggtgacg tttcacctcg aaaactccgg cttgaaccgt attctggtcc
      301 accgggatca ggatgtgcgt tcgaaagatg gtcatgctcc cctcctcttc tcctccagga
      361 tttctcggat ccgttgcgga gtcaggttgc cgtatacctc gtcattgacc atcagggccg
      421 gggcgacccc acagactccc aggcaggaag acatctccag ggtgaaaaga tcgtcccggg
      481 tggtttcgcc gaactgaatg cccaatagtt tcatcagctc ttgggcgatc gtggtggacc
      541 ccaggaggtg gcaaggggga gactcgcaga cccggatgat gaatttcccc ctcggcgtga
      601 ggctgaacat ggtgtagaaa gtggccactc cctgaacgaa actgaagggc agccgcaggt
      661 ggccggcggc gagctgcagg tcctgctcgg tcaggtagtg gcgttcgttg gcatcctgaa
      721 gatcgtggag gatgctcaag aggttttccg gcgcagggtc atactgctgg atgatcttct
      781 ggggagtcat gtttcacctc tttgagaaat tatacggctt ccgcatggcc gatgtcggtc
      841 tcctccccgg cggcgaccgc ggtctggttc cggtcgcagt gcatgcaccg ccgggcctct
      901 tccatcactt cttcgcgcag gaaccccagg ttgacctcca gagccaaggt gcggattctt
      961 ttctccacct cttccagccg gggggttttc cgggggatat tcttcaggta ggcctcttcg
     1021 tcgtagctca ccgccacggg ctttcgctcc ctggcgaaga gtttgccctc gtagccgaag
     1081 tgccggtcga tctccatggc cgctttcctt ccctgggcca cggactcgat gatggtcgag
     1141 gggttgaccg agtcccctcc ggcatagacc cccggcaagc tggtggccag ggtgcgggca
     1201 ttcacttcca cctgtccccg tttgtccagc ttgagtttcg ccccgttcaa accgccgctc
     1261 agcttctggc cgaggcagag gatgatcgtg tccaccggga tggtgaactc gctcccctgg
     1321 atggggacgg gtttcctccg ccccgatgtg tcgaactctc ccagctcggt gcggatgcag
     1381 gtgagcccgg tcactttccc gttcttcccc tccaccttta ccgggttcac caggaaatcc
     1441 atctggattc gctcctcgtc ggcttcctcg atttcctccg gccaggcggg catctcggcc
     1501 cgagtgcggc ggtagacaat ctggaccgac tcggccccca gacgtttgga gacccgggcg
     1561 gcgtccatgg cagtggaccc accgccgatg accaccaccc tttggcccac attcactttt
     1621 ttccccaggt tcacctcgcg caggaaatcg atccccgaga ggacaccttt cagatcgatt
     1681 ccgggaacgc tcaagggttg gggcgtccag gctccggttc ccaggaatac ggcctggaat
     1741 ccctgcttct gcaggtcctc gacggatttc actgggctgt tcagacggat ttccaccccg
     1801 gcccgctcga tgaccccgat ctctgcggca agcttttcct tgggcagccg gaaatcgggg
     1861 atgcccagct tcatcattcc cccgggcacg ggcatggcct caaagaccac gggcttgtat
     1921 cccatccggg tcaggtaaaa tccgcaggcc agcccggcgg gaccggcacc cacgatggcc
     1981 acccgttctt ttttggggct cttggggggc aggaacccga tgtcctgttc caccgcccag
     2041 tcggcgaaga gccgtttgat ctcccggatg gcgatggcct gatcatattt acccctggag
     2101 catttctttt cgcagaaggc cgggcagacc cgggagcagg cgatggggaa agggttattt
     2161 tccatgtgcc tcaggtaggc ctcctgcagc cggccctcgg ccatgtagga gacgtaggag
     2221 gcggcgtcca cattggccgg acaggcattt tcgcagggag cataaaagag cgccgcgcag
     2281 accccggccc ggcaggcctt gtcctgaatg tgctccaggt actcatggcg gaagtactgg
     2341 agggtggaga tgaccgggtt gggcgccgtc tggcccagac cgcataggga agtgttcttg
     2401 acccagtggg ccagcttttc aagcttctcc aggtcttcca gggtccccaa ccccagggtg
     2461 attttttcca aaaggtcccg catctgcttc aggccgaccc gacagggaac gcactggccg
     2521 caggactcct ccatggtgaa atcgaggaaa tacatggcca tgttcaccat gcagctgtct
     2581 tcgtcgagga cgatgatccc gccggacccc atgatggagc cgatctgctt cagcgactcg
     2641 tagtcgatgg ccgagttcag gtatttgggc gggatgcacc caccggaggg gccgccggtc
     2701 tggaccgcct tgaaggcctt atcgtccggg attccgccgc cgatgtcgaa gaccagctcc
     2761 cccagggtgg tgcccatggg cacctcgacc agtccggtgt tgttcacttt cccagaaaga
     2821 gcgaagacct tggttccctt gctggtggcc gtgccgatgg aagcgaacca ctcccagccg
     2881 ttgaggaaga tatggcggat gttggccagg gtttccacgt tgttgatgat ggtcggctgc
     2941 tgccagaggc ccttgctggc cgggaaggga ggctttggac ggggaagtcc ccgctgccct
     3001 tcgatggagg caatgagcgc ggtctcctcc ccgcagacaa aggctccggc ccccatgcgc
     3061 agttccaggt cgaattcaaa gccggtctcg aagatgtcct tccccaagag ccccatctgg
     3121 cgggcctgct ggatggcgat gttcagtcgg tcgatggcca gggggtactc ggcgcgcacg
     3181 taaatgtatc cccggcttgc gccgatggcg tagccggcga tcgccatccc ttccagcacc
     3241 gcatgggggt cgccttcgca gaccgcccga tccatgaagg cccccgggtc tccctcgtcg
     3301 gcgttgcaga tgacgaactt ctggggtgcc ggattggcgc gggcgaattt ccatttcaac
     3361 cccgtgggga acccggcccc gccccgtccc cgcaggccgg acttggtcat gatgtcgatg
     3421 acctcctccg gtttcatggt ggtgagaatc gttcccaggg cttcgtagcc ccgctgggcg
     3481 atgtactcct cgatgttttc cgggtcgatc ctgccgcaga gcgccaggac gatcttcttc
     3541 tgcttctcga agaaggggac ctgtttttgt tcctccacga tcttgcgggc ctcgggggtc
     3601 gtccagagaa gggactgaac ggggcggccc ttcagaaagt gttccaggac gatttcttcg
     3661 gcatgctctt ccttcaggtt gatgtagaac gatccgtcgg ggtagaccac catgaccggc
     3721 ccgagctggc aggggcccat gcatcccgtt tcgatgagat tgacctcttc ggtcaagcct
     3781 ttttggttga tttcctcgga cagtttattc ttaaactcgc gactgtggga ggagatacag
     3841 gcaccgcccc cgcagacgag aatatccaat ctcggtctca taccatcctc gcagtctatt
     3901 tttgggtttc gatcacgtaa tcggaaaccg ggttgccgtt gaccaggtgt tcggccacga
     3961 tctttcgggc gatatcggcg gaaacgtcgc cgtaaacgac cgagggcttc ccttccaccg
     4021 tgacgttgac gatgggttcc ttggacgaga gcccccgctc cccggactgg gtcacgatca
     4081 cgtccttcag ccctctcctc tcgatctcct ccagaaaggc cttcatggtc tgccgggcac
     4141 cggcggcgat gccgctcgtt cccatgctga ctgcaacctt gaccctggct ttcccgtccc
     4201 ggagaaccat atctttttct cttttttccc tgagtttctt caggtcttcg aggttcattc
     4261 tatcctcctt tgatcaaatt atgccgtatg gatgttgctt ttgatttcct ggatgatctg
     4321 gttcgccttg gtggggttca accttccgta cacctcgttg tcgatcatca tgcagggagc
     4381 cagcccgcag gcgccgaaac agcgtgcgga ggaaagggtg aagaagcggt ctggcgtggt
     4441 tcctcccatc ttgatggcca gagatttctc cagggacttg atgatttccg aggcgccttt
     4501 cacgtggcag gcggtcccca aacacacatt aatgagatgg tcgcccatgg gctcggtgcg
     4561 gaagaagtta tagaaagtga ccaccccgtg tactttggaa aggggcagcc ggagttcccg
     4621 cgccacatac ttctgcacat cctccggcag gtagccgaaa attttctgcg cccggtgaag
     4681 cacctgaatc agcggacccg cgatctggcg gttggcgtcg atgaactgct gcagttcccc
     4741 gtatcggaca ctgtccggtt gttgaacctg ttgcgtctcc ataaaagcct cctctaggga
     4801 tgatatccgc acgattcgcc gggacctttt ggatgattct ggttccttcg aggctccgga
     4861 tcgaccgcgg gatgcgactt agccgaatcc ggcttcttcc tttccgcaaa gaagaaacgg
     4921 aatccagcag gaaaagggaa gaaatctttt ctcgtgagga aaagcgcgga agaatcgaaa
     4981 tcgtcgagga aaattctttg cggggacagg gtgatggttg ggatcatttc aacggccaga
     5041 tgcccttacg acgatcgacg gtctccgtcc gtgtcaacat ccttattttt cccgaagcgc
     5101 agcgttgtcc tgacggcgga ccagccccgc cgaaccaagc tttccttaac cactttaatt
     5161 cttaagcttt tctttcctca gccggcttaa aaaagcgata tatatatatc aaaaaagaaa
     5221 ccaatttgta aagaaaaaat tgttcggatt cgaacttttt tcgtccccca gggaccccca
     5281 gcggcaggtg caacccgggg agaaaacatt cggaagcctt gcccgccact ccagaatctc
     5341 atccctgggg agagccgggg aaaatcaatt tcaagacttt ccttccttct ttcccctgga
     5401 aagccagccg caactcgccc agcgtcggtt cagccaacaa aaaaaccgta cgagcccggc
     5461 caatgtcatc gacctggtgg gtgtcggagc agacaacccg gggaacccgg gccgagggat
     5521 cccggggatt ggccgcttgg gcagcctgat tttttcggcc catctccagc gcctccggct
     5581 ccagatcaaa ggggacgaaa cccaattggc tgatgaggct gaaggaatcc ttgtcaaaat
     5641 gggctgggat gaagagccct cctcgctgga gcacctggcc gcgaatctca ttcagggaat
     5701 agtcgatgga attgatcagc agccgctcct cgaattccag gatgttaccc tcctcgtcca
     5761 cgataggctg gtcgccgaag aattccgggt cgttgggaat ccccgggaga tgctcaagaa
     5821 ccacagcctc ccaggcactc aaggaaggcc agtcgggaaa aagggttaga atatgaattt
     5881 cttcccggga ctggacctcc atcccgggta tgatttttaa accgaatttt tcggcctgat
     5941 gcatcaccgc cggaacattc ttggcaatgt tgtgatcggt gatggcgatg aggtccagcc
     6001 cttcctccag ggcccgctcg acgatcctgc ggggagaaag atccaggctg gcacacggag
     6061 aaaggcatgt gtggatgtgc aaatctgcgt gatactcctt cagcatttct cccgaatccc
     6121 gaagaggaca gggtttctcg cctctccccc gatcctcccc cccgggctaa ggtcccctct
     6181 cctttccgag agggcctctc cgacggaccc tgccgaaccc aatcttattc tggccggaaa
     6241 gaagcgatcc gggtatgact cacatcagag cgaggaccga ttgccgggcc aaattcaaat
     6301 aattcgccgg gaagatgccc atctgaaggg tgaagaagac ggcgatcaga agagcgataa
     6361 ccattccggg agagaaatcc aggcccccgg ctgcctcctt ttcggggccg cgcatataca
     6421 tcatcaccgt cacgcgcaga taaaagtaaa cggacagggc gctgttcaaa accccgataa
     6481 tggccagccc cacgtagccg gccttgaccg ccgcgctaaa gatgtaaaac tttcccacaa
     6541 agcccgctgt tggaggaatg cccgccaggg agaacatgaa tactgccatg gcggccgcca
     6601 ggaggggata acgggaggcc atcccgctgt aatcgctgac cagaatattt tcctcgccct
     6661 tccggccgta caggatcacc accccgaaag ctcctaggtt catgaaggcg taagccagga
     6721 ggtaataaag aatgctcgcc gtactcattt cgctggaagc aaccatggct accaggatgt
     6781 aaccagcgtg ggcgatgctg gaataagcca gcattcgctt gatgttcttc tgggcgatgg
     6841 ccacgatatt tcccagcgtc atggtcaaga cggcgaggac ccagaggacc cagacccatt
     6901 cagtctgcag ggaggaaagg gaatacagga agactcgcag aaaagcagca aatcctgcgg
     6961 ctttgggccc aacagacatg aaggcggtga cggccgtggg ggccccctcg tacacatcgg
     7021 gagtccacat gtggaagggc acggaggcca ccttgaaacc gaaccccacg atcaggagtc
     7081 ccatgccgat gagcagcagg gggtcggtga aaaggtccgc ccggcggaga aaatcaaaga
     7141 tccccttcag gttggtggtt cccgtggccc cgtagatcag ggcgattccg taaagcagga
     7201 atcccgcgga gaaggcgccc aggagaaaat atttcaggga ggactcgttg gatttcggat
     7261 tcgtccggaa gaagccggcc agcgcataga cggcgaggga gaaggtttcc aggcccaaga
     7321 agacgataat caagtcggcg gctgaggcca taaacatcat gcccacggcg gcaaaaagca
     7381 agagaatgta atactcgccg taatcgaatc cttccgctct tagaaaccga atggacatga
     7441 ggatggtcag accggcggtg atcaggaaga tggctttgag gaagagcgaa tagttgtcca
     7501 ggaccaccat gcggttgaag gcatactggg actggcccca gaggctgaag cagaggacca
     7561 gggaaatggc caccccgatc aggctcagga tggggatcgc ccccttggcc catttcttgg
     7621 cgaggacgtt cagcaggaga agaaccatag ccgtcaggct gaggaccaac tccggcccga
     7681 ttacgctcca attgatgatc gggatgacca ccttcattta ttccctccga aagaaaagtc
     7741 cgttatccat atggaatttc ttaatacttt aaagggctga gaaatcccaa attccaagca
     7801 ccaaatcaca aacaatcacc aatactcaag cacccaattc ccaaggggtc gtcattccgg
     7861 cgtaagccgg aatccagttg tttaaataag tactggaccc cggctttcgc cggggtgacg
     7921 cttaaagaga ctttttacgc aatcatccca atttgattct cccaaatcca tcttttcttc
     7981 attcgagatt tggtgcttgg aatttatttc ttttccgcct gggctacctt gatcttttcc
     8041 ccctcctgca tctggacccc cgcctggtat ttctgctgca cttggaccag gtaagccttg
     8101 actgtgggtt ccatccggct gaggaaggtc tgggggtaaa ccccgatcca gaagatgaac
     8161 agggttacgc aggaaaggac caagacctct ctcttggaga ggtccttcag caccttgttt
     8221 tctggtttgg tcagctcccc gaacatgacc cgctgaaaca tccaaagcat gtaggccgca
     8281 gctaggatga cccccgtggc cgccacgacg gcataaggcc cattggcttt aaaggttccc
     8341 accaagatca gaaactcgcc tacaaaaccg tttaaacccg gaagcccaat cgaggagagg
     8401 gtcaccacca tgaagaaggc ggcgaagatg ggcatctgct tccagagacc gccgaagtcc
     8461 tcaatcatgc gggtatgcct gcgctcatag atcatcccca ccagcaggaa gagagccccg
     8521 gtggagaacc cgtggttgag catctgcagg attccgccct ggattccctg gatgttgaag
     8581 gcaaaaaggc cgagcatgac gaatcccaga tgggaaaccg aggaaaatgc gatcaggcgc
     8641 ttaaggtcct tctgcaccag gcagaccaga gctccgtaca cgatcccgat cagggccagg
     8701 actgagacta ggggaatcag ctggaaagag gcttgaggga agaggggaat gctataacgc
     8761 aagaatccgt acgttcccat cttcagcagg acagcggcca ggaggaccga tcccaccgtg
     8821 ggggcctcgg tgtgggcgtc gggcaaccag gtatggaagg ggaacatggg aaccttgatc
     8881 gcaaaggcca ggccgaaagc caggaagagc cacacctgaa tatcccggga cacctggagg
     8941 ttatagagct ggaggaggtc gaaggtgtat ttcccggtgg ccgccccgtt atggaagtag
     9001 aggactaaaa tggcgaccag catgaggaca cttcccacca tggtgtagat gaagaacttc
     9061 actgcggcat agatgcgccg tttgggattg ccccacaccc cgatcaggag atacatgggg
     9121 ataagcatga cttcccagaa gacgtagaag aggaagaggt ccagggagac gaagaccccg
     9181 atcattcccg tctccaggaa aagaatgcag atcatgaact ccttcacctt gtcctggatg
     9241 tcggcccagc aggccatgat cgagacgaag gtgaggaagg tggtaagcag gacgaggaac
     9301 aggctgattc catcgatccc caggtagtag gtaatcccgt actccgggat ccaggaggcc
     9361 ttctccacga actgcatggc cgccgtgtcc agccggaagt tgaagaagag gggaagggag
     9421 aagagaaact ccagcaggga caccccgagg gtgacttggc ggatcgtgtc tttcttctcc
     9481 ttgtccaaaa agaggaggat caaaactccc accatgggga agaaggtaat caacgtgaga
     9541 aggggaattc ctgccaggct gatcatgaaa accgctcctt ttttgtaggg cctgatcccc
     9601 gatcaggccg tcagacggtg cgttcgggga acgcacccta cacat
//
LOCUS       DASPRR010000122        51990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1319584, whole genome shotgun sequence.
ACCESSION   DASPRR010000122 DASPRR010000000
VERSION     DASPRR010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 51990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51990)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51990
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319584"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VEL68_04280"
     CDS             complement(<1..471)
                     /locus_tag="VEL68_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35220.1"
                     /translation="MNFELLMEGPYDLTRCAWAFCHFPQDGTDVWIPPRETLPAEYHR
                     LHVVEGEPILTRVQQEFGSKNDRVRLIVRTFPEQVRKVSLLKERVRWQFHVEASLEGF
                     YQRARKHPFLRPLVKALYGVKPIRPSTVFEMAVIALSEQQISLNAAIQIRSRLVR"
     gene            814..2547
                     /locus_tag="VEL68_04285"
     CDS             814..2547
                     /locus_tag="VEL68_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXZ35221.1"
                     /translation="MRPYFEKMPAFGKPLSERQMQSSEENIKKIREVEQEIAEAVKKV
                     KGAGISTEEINKRGEMTVYQRLEYLVDPGTWCPLHTLYDPMEEESGTTGVVDGLGKIG
                     GKWAVIIGFDNKVMAGAWIAGQSDNVLRVTDMAKNLHLPLVWLVNCSGVKLPEQEKVY
                     ANRRGSGTTFFRHAELEKLGIPVLAGIYGTNPAGGGYQGISPTILLAHKNCNIAVGGG
                     GIVSGMSPKGFFDEEGAEVVIEATRKFKAVPPGRVEIHYDSTGFFKAVFQKEEGVLDG
                     LKEYMKYLPAYDPKFFRVAEPKAPKFSPEEINRLVPFNQKMAYNFEEVLARLTDNSEH
                     MEFRPTYGPEVYTGLAKVDGLVVGIIGNKQGILPKGYPEYAPYPGVGGKFYRQGLIKM
                     NEFVTQCGRDRVPIIWFQDTTGIDVGDLAEKAELLGLGQSLIYSIEQSNVPMMCVILR
                     KGTAAAHYIMGGPQANNNNIFTLGTPTSEIYVMHGETAAVASFARRLVKEKDSNRPLK
                     PIIDKMNALAQDYYNKSRPIYCAKTGFVDEVVPLPEIRKYMVAFTGAAYQNPRSICPH
                     HHLMLPRLIKG"
     gene            complement(2604..2984)
                     /locus_tag="VEL68_04290"
     CDS             complement(2604..2984)
                     /locus_tag="VEL68_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ35222.1"
                     /translation="MKIFRLKDLDWIPASHESLQSPSVWKKVLLQKDDLLEGRVQMVN
                     WCRMEPEKAFQAHYHEDMEEIFIILKGQAKILVNGEEAEMGEGEAVIILPREIHQMKN
                     GGGEDLEYLAVGISQGIGGKTVVV"
     gene            3452..4630
                     /locus_tag="VEL68_04295"
     CDS             3452..4630
                     /locus_tag="VEL68_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35223.1"
                     /translation="MKKMLGLTFILFLAFGSLGWTQDTIKIGVVGPRTGPAAATGKAF
                     EEGIAIALDHINAKGGVLGKKVEVIFEDTGGVPEKAASALERLATREKVPVVVGESHS
                     SSALAEIEVANRYKIPLIICEAWHDDITKKGYKWVFRAGPANSGVVDFYIAPFVKENG
                     FKKAAIVRENSDWGAGIAERTEMNLKSMNIPFTTLKVERESKDYYTELTKLKSENPDI
                     ILAYIYGTGLHFFISQASEVGLSPKALILDGAGPPSLWDSYWKNVGEAGELELFMSSM
                     HEKVHLTATSRKFWDDYKKKYGKDPSDYKTRSIYNVILIAADAINRAKSTDPEALVAA
                     LEKTNLEVTTGTVKFGMKEGSYEYHQWMPPMLVVQWQNKQQVVVYPKEAATGALKKTK
                     "
     gene            4725..5585
                     /locus_tag="VEL68_04300"
     CDS             4725..5585
                     /locus_tag="VEL68_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35224.1"
                     /translation="MAEFLMYLTNGIVIGVINAISAIGVSLIVGIMKVVNFAHGEMYI
                     FAGYFSYHLSTSLGLPPVLSMIVAVGFVFLFGLAMERTLIRPTYGDDMYSLIITFVLS
                     IVLQNLALLIFGPYPNKPPNLVSGATNLFGLFQYGNQRLVSFFISIGILIFIFYLIKK
                     TWFGKSIRAISQDREMSSLMGVDHLRMNMFSFGLGAALAGAAGVILAPVFPVTPTGSS
                     SISLTAFVVVVLGGMGSLKGCLVGGLILGIIENLGAAYISTMYRHIFAFVILIMVLVF
                     RPWGLYGQKT"
     gene            5704..6618
                     /locus_tag="VEL68_04305"
     CDS             5704..6618
                     /locus_tag="VEL68_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35225.1"
                     /translation="MDLKKLWWIPILAFLAILPPFVLSQYWLHTMIISLFYVMMAASW
                     NLLAGYTGQVSFAHAAFCGVGAYVSGMLAVFLGVSPWLGILSGTAMAALLGFGLGFLC
                     IRMGGIYLSLTTLGFSEILRIIITNEEKWTRGTMGLQTPGLFAKYSKTDYYFVFLVAT
                     VVILIFIHRLIRSEWGLTFRAVLNDEVAASSSGINTIRVRVLAFTLTSGMAGLAGGLY
                     GHYLMLITPDIPSLGQMFLVLAMAVIGGMGSFAGPVIGSFVLEILSEYIRVYGEYHVL
                     LFGLVALAVARFAPEGIMGTWAKMRRSP"
     gene            6615..7352
                     /locus_tag="VEL68_04310"
     CDS             6615..7352
                     /locus_tag="VEL68_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35226.1"
                     /translation="MSAILEGKNLTKSFGGLTAVREVDFHLEQGEIVGLIGPNGAGKT
                     TLFNLISGVFPTDTGTLRFNDREIGGLRSFEICRLGIARTFQIVRPFLKMNCLENVLV
                     GIAGREEKETRRKEEKREEAWELLKFIGLPGEEYRLAQNLTLMQKKRLEMARALATRP
                     RVILLDEVLAGLNPSEMHQALDLIEGIRSEQKITIFWIEHVMGAIMKGSDRIIVLDQG
                     RMIREGKPEEVVSDPRVIEAYLGDANA"
     gene            7345..8049
                     /locus_tag="VEL68_04315"
     CDS             7345..8049
                     /locus_tag="VEL68_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35227.1"
                     /translation="MLEVKGISVFRGQAQILWGISMQVEKGEVVTLLGANGAGKTTFV
                     ESILGIHHPPSGAIAFLGSDITRRTPFQITRLGVSCVPEGRRLFKDMTVYENLEMGAY
                     PPRARTVLRSTVQWVFSLFPVLDQRKMQIAGTLSGGEQQMLAIGRALMSKPQLLLMDE
                     LSLGLSPKITKGIYGTVDKLRREGVTLLLIEQNATLALRHSDRGYILETGRITLQGTS
                     QELLGNEHIRKAYLGM"
     gene            8099..8920
                     /locus_tag="VEL68_04320"
     CDS             8099..8920
                     /locus_tag="VEL68_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXZ35228.1"
                     /translation="MDKMIITVGVTGSRISRKQTPHIPILPKEIAQSGVEAVKAGASI
                     LHIHVRDPQTGLGTQDHAIFQEVVDRVRSEVDCVLCLTTSGIPGRNLPIEQRLTPLAL
                     KPELVSYDAGSINMGENVFLNPPDFLEALAKGCLERGVKPELEIFEVGMVYTCLKYFE
                     KGLLKPPLHFQFVLGTPAGMPATPKSLIYLTEIIPSDSTWSVIGIGPGQLPLAVMGMA
                     MGGHVRVGLEDNIYYSKGRLARSNSELVERVVKIAREYGREIATTREARKILSLV"
     gene            9192..11492
                     /locus_tag="VEL68_04325"
     CDS             9192..11492
                     /locus_tag="VEL68_04325"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HXZ35229.1"
                     /translation="MRITLRIILSIFFVISVIVLLFTFLQVEQEKERMTLDLERRASI
                     LGESLKESIEPLVARRQSSSVQKIVDKFGNRERLAGVAIYDAQGKLSAVTVQLTPYLK
                     TPPSIVENVMKAAVEEGNFLPLGEKEAYLYALPLFNEEKVAGAMVMVYETNQIKDRVA
                     LIWQNNFIRLLVSALFISLTTLLVIRWSMVHPIARMAEWMKTLRTGRGGAPLPLPPGK
                     VFEPLAREATRMVKSISEARSAAEQEARLRRKAESLWTPVRLCEHVRTLLQGKPLFVI
                     SNREPYVHERRGKKIECVVPASGLVTALEPILQACGGTWLAHGSGDADREVVDDQDKV
                     RVPPEDPQYLLKRVWLTKEEEEGYYYGFANEGLWPLCHIAHTRPTFRAEDWNHYEAVN
                     WKFAQATFEELEKTQDPWILVQDYHFALFPRIIKEKRPDARVALFWHIPWPNPESFGI
                     CPWQKEILHGMLGADLIGFHVQFHCNNFLETVDRALESRIDWENFTAERENHLTQVRP
                     FPISIAAPNPSSSFRSRNGGLKKALLRELGIQGKYLGVGVDRIDYTKGLRERFLAIER
                     FLEKYPVYQGMFTFVELGAPSRTHIKEYRDLTAQVESEAERINWRFQAKDWRPIVFLK
                     RHHSHKEIAPFYEAADLCLVTSLHDGMNLVAKEFIGAKWDESGVLILSQFTGAARELR
                     DALVVNPYDTEQMAEAIRLALEMDPAEKRARMHRMREAVKEKNIYRWAGDLISSLSQI
                     RLSSVPPLGISAPYNSSRPNNLPGVG"
     gene            complement(11440..12639)
                     /locus_tag="VEL68_04330"
     CDS             complement(11440..12639)
                     /locus_tag="VEL68_04330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ35230.1"
                     /translation="MSRLEAYREIAPQGSVEFLLRMAEKIGKKRILHMNSTKLGGGVA
                     EILRSLVPLLESVGLEAQWDVITGNEEFFSTTKAFHNALQGQEQRISPEMLQNYLEIN
                     RENAKRLSLEADFVIIHDPQPAALIESRRNKKGKWVWRCHIDVSQPQRKVWDFLRQFV
                     VKYDAAIFSLPRFAQRLPIPQFLIYPSIDPLSDKNRGISPQELDQILSRLKISLDKPI
                     LLQVSRFDRFKDPLGVIQAYRLVKKHHDLQLILAGGGATDDPEGEAVLAEVRQAAQDD
                     PDIRVLVLPPDANLEINALQRVATIVLQKSLREGFGLTVAEAMWKGKPVIGGFTGGIT
                     VQLVYGVTGFTVNSVEGTAFRIHYLLDNPEAMARMGQDAREFVRRNFLITRHLGDYLA
                     LMSYMGR"
     gene            complement(12636..13313)
                     /locus_tag="VEL68_04335"
     CDS             complement(12636..13313)
                     /locus_tag="VEL68_04335"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HXZ35231.1"
                     /translation="MDSRTAEHPFVFVGCWELREMLGRSARDERQLLEAIEEVPLDSI
                     YYHTHSFLLRHKYIAGPYPNDFATWAAIQVRDRVLGEKLGILSPYDFQNLESLRAEIV
                     TIISDHLSRLQIIPRVIYEEPFHFMQSRIISAPTGLQVHTLAEFRKVLATVDDSVIYY
                     HTFEAILRLGKPKGDFALWMEEQLGLPELGKRISNIDPYRISLESARSRILNLCDQFL
                     QEGRDRK"
     gene            complement(13353..14138)
                     /gene="otsB"
                     /locus_tag="VEL68_04340"
     CDS             complement(13353..14138)
                     /gene="otsB"
                     /locus_tag="VEL68_04340"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HXZ35232.1"
                     /translation="MKHLYEAWDEVEPRIRNARLLFLLLDYDGTLCPIVPRPDLAVCP
                     PQVKVLLEKLRDSPKVRVAIISGRALEDLKDKVRVQGIIYVGNHGLEVENPAGTHRKK
                     LSTERQDELRKIRDSLETNLKLIPGILFEDKGPILTVHYRNTPREFTPRVQKAVEEVL
                     QPFPDRWQSSPGKKIVEIRPKVGIDKGKTVKEILIHFPSVNPLPVYLGDDRSDEDAFR
                     VIGSQGISVWVGPGAASSSAQYFLRDPEEVRGFLQRCQEILEN"
     gene            14449..15747
                     /locus_tag="VEL68_04345"
     CDS             14449..15747
                     /locus_tag="VEL68_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXZ35233.1"
                     /translation="MKRLLIYSLVGVMIMVFAAGTALGQAQKEAFKYEDKIGVIKLKS
                     GEPIHIACWMVIAGPDASLGTDTKRGVEIAIDDKGGKLLGHPIKLTAQDTGCNAEGGQ
                     AAATKLAADPTVVAAVGSNCSSEARPGAPILWKAGIATVSPSNTAPYLTDPNRGPDYD
                     GYLRTAHNDKVQGAVAAEFAFKQLKVKKAATIHDGSVYAEQLQAVFAETFKKMGGTIT
                     SQEAVAPTDTDMRPVLTKIATGNPEFLYFPIFIAAGGHITRQVKEVKGLEKTALMGAD
                     GIFSTDFYKAAGETAVGMYHSSPDFSAFGAGYKAFLDKHQKKYGEKPIAPFHAHGYDA
                     AMMIFAAIQKVAQKAPDGTLYIGRQKLRDALYATKGLRGLTGTITCDKYGDCADPRIA
                     VYKTTEENVKKLVMPEKPFWAPVAAAPAAKPPVQKAPAKK"
     gene            16060..17121
                     /locus_tag="VEL68_04350"
     CDS             16060..17121
                     /locus_tag="VEL68_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35234.1"
                     /translation="MRQQWRERISLTDVLMWLIGGAILLLILWGSTATLVSGRYSGHH
                     WFDFMVFGLAQGSIYALIALGYTMVYGTLRMINFAHSEVFMGGPYTAYFVAAAFYSSG
                     FLDQHPIPSLIVVFFVSMATSTAIAVLLERVAYRPLRNSPRLVPLITAIGASFFLQYT
                     FRGLYGSGFQAYPVVKVLEGQWMLGEFRILKFQAVVIIAAALMMAALYAFVQKTKMGK
                     AIRAVSEDKEAASLMGIGIDRMIVTTFVIGGISAGAAGVLYALMFKQVHFFMGFIPGI
                     KAFTAAVLGGIGNIPGAMLGGIFLGLVESVGPSLFLDGLGIVAPYQLKDAIAFTMLVL
                     VLIFRPTGLLGERLAVKKA"
     gene            17925..18929
                     /locus_tag="VEL68_04355"
     CDS             17925..18929
                     /locus_tag="VEL68_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35235.1"
                     /translation="MPPERQRLFRLAGWGLLALLLFLLPQVLGLFLSEVLTIVGLYVL
                     LGLGLNIVVGYAGLLDLGYVAFFAIGSYTMALLTSPELGFFHFSFWGALPFAIFMGVI
                     FGVLLGIPVLKMRGDYLAIATLGFGEIIRILVLSDFLRPWLGGAQGVGKIPKASLGAI
                     EFATPQQLYYLILAGCLLVGFISWRLRDSRLGRAWMAVREDEDVAQAMGINLVATKLM
                     AFATGAGFSALSGAIFASKLGSVYPHSFNVMISINILCVIIVGGMGSIPGVILGSIAL
                     VGLPELLREFAEYRLLVYGAALVAMMLLRPEGLWPEAVRRRELHEEEAEEVETQPPPA
                     "
     gene            19148..19921
                     /locus_tag="VEL68_04360"
     CDS             19148..19921
                     /locus_tag="VEL68_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35236.1"
                     /translation="MAILAAKKATKRFGGLLAMQELDIEVQEKSIHSIIGPNGAGKTT
                     FFNCVTGFYKAEEGEILFNGHSLVGLLPDQIVRLGITRTYQNIRLFPDLTVMENVLVG
                     LHGHLKAGFFGIVVGTPGTRKEEKEAAEAARKILRFVNLAGKGDFLAKNLPYGDQRRL
                     EVARALATRPQLLLLDEPTAGMNPKETQEMMDFIRHLRDELGITILLIEHQMRVVMGI
                     SETVTVLDYGKKIAEGSPAEIRSNAVVIEAYLGKGGGSH"
     gene            19930..20649
                     /locus_tag="VEL68_04365"
     CDS             19930..20649
                     /locus_tag="VEL68_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35237.1"
                     /translation="MAILEIEHIHSFYGHIHALKDVSLVVEKGEIVTLVGANGAGKST
                     TLKTISGLLRPKQGTVRFEGEELTQYPPHEIVGKGVVQVPEGRRIFGRLTVLENLEMG
                     AFLLSDRKRVQANLKQVFSLFPRLEERLDQVGGTLSGGEQQMLATGRALMSSPRLLLL
                     DEPSMGLAPVLVDSIFDTIRKLHETGTTILLVEQNARMALQVADRGYVLQTGTILLSD
                     SSESLRQNETVRQAYLGESTE"
     gene            20965..23190
                     /locus_tag="VEL68_04370"
     CDS             20965..23190
                     /locus_tag="VEL68_04370"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ35238.1"
                     /translation="MSVYAIPAALAFGINLTLCLIVFYDNPKGVVNRVFTLLILSFVA
                     WNAGELIMITSDLHSVALVGVKVIFIGVFLFPVFFLHFSYVFPQKITPLFDGWRSLIL
                     YAGPVALLVPFILLLQVDIGRVVQIGSIFYYVFTFHGRLESQLMALILGLIGLGYFTW
                     GVVNFIISYRQTKIARQRLQILYLLVGIISMFVIGLVLHVMNYFFDLGYSFFFVASLY
                     SLLISFFFALAIVKYRLVDIHFIIRGGILYSLLSGLVLVIYVLFIKNVCDILARKLET
                     TSLLVEATMVLVLVFLLRPLERKVEEFIDRFFYRERYFFRLKFFEFTRTLLNLLDLQT
                     LLETTVAFISQALLPDRVGIWILESESNCYRLFSQKGFSRERYFAADSPFIAYLKKSE
                     RAVELETVKDFRALQPEVREMSDARLSLVLPLEWEEGLLGFILVGEKKDGKNFTIMEI
                     EALEALAPAVAMAISRARLYQDLKKKDWQMMQSEKLAALGEMAASFVHGIRNPLGIIL
                     GSAETLKKKVPQPVQSEMIQFISEESDRINRMLTNFLEFAKPKPPVFQEVDLKEVLDR
                     TLDWISIPARKQRVEVLREYPEEKVLIPVDPEQVREALVNLEMNALEAMPQGGKLRIV
                     LNQKDNRDVTIRLSDTGGGISPELESKIFDPFFTTKQKGTGLGLSIVHSVVKNHGGTI
                     SVISNNGEGTTFTLTLPFPKAIMGCFGAVGPVQNSKEQGSGHIPESSARSSERKGRSN
                     E"
     gene            23261..24664
                     /locus_tag="VEL68_04375"
     CDS             23261..24664
                     /locus_tag="VEL68_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ35239.1"
                     /translation="MKPRILIVDDEARMQRLFEINLGPKYEVMTAGDGEQALEVVKSG
                     DVTLLVTDLKMPGMNGMTLLQEVHRVYPDLPVVIMTAYGTVEGAVQAMKEGAVDYILK
                     PVKMEEMEFLIEKTLSVRRLQDENRSLRQELQSVYGPASIVGNHPAMQKIIQLISQVA
                     GTKATVLVQGESGTGKEIIARAIHYQSDRASKPFVVINCAAIPSNLLESELFGHEKGA
                     FTGAIKTKKGRLELADQGTLFLDEIGEMPKELQVKILRVLEEQKFQRVGGTEDVEVDN
                     RIIAATNKDLKQAVEAGAFRDDLFYRLNVITISIPPLRERKEDIPLLVDYFLEKHRKI
                     FKSRATGVSDEALKLLAEYPWPGNVRELENTLVRAMILCGSELIQPGDLPEGLGPEEI
                     EEEVRPPTDREELKQMKKVAQQKAKGEIEKKFIIEALKQGGGNVMRSAEMVGMDRRQF
                     QNLLRKYGISRKDFLKD"
     gene            24926..26578
                     /locus_tag="VEL68_04380"
     CDS             24926..26578
                     /locus_tag="VEL68_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ35240.1"
                     /translation="MAIGGLIRGLRKKTAFKDWRKIEPYYPPTQNVPLGLPKEVESLC
                     PECGKIIRASILERGGKVFMEKTCPDHGFLRDLVYSDAELYKKCERWTYEDADGILNP
                     QVTGAKVCPDDCGLCDLHISPPILANIDLTNRCNLRCPICFANANVQGYVYEPSYEQV
                     VEMLTLMRNVKPFFPPAIQFSGGEPTIHPRFLDILKAGRSMGFSHLQIATNGIRIAKD
                     PEFAKKARESGLYTMYLQFDGTSDDVYKKTRGLSGLWELKQRAVENARAAELRVVLVP
                     TIIKTINDDQVGNILQFAVDNSDVISGISYQPVAFTGRISTEERERQRYTLSDLAIDI
                     EAQTDLLRAREDWYPLSVTSPFSKLLSSIWKYDVAKITSHSDCGIGSYIFVNRKTRSV
                     TPITQAIDIEGFTSELNKLVKQKISNIRALTALSAQHLLRKFFREDRAKDGLTAEICL
                     ELFQGVVDKEAQRRVELIYNWDMLLVGGMHFQDSYNYNVARVKRCCIHYAAPNGRIYP
                     FCTYNSGPVFREKIERQFSVPLEEWRKRNWAQEDADYYLCRT"
     gene            complement(26719..27126)
                     /locus_tag="VEL68_04385"
     CDS             complement(26719..27126)
                     /locus_tag="VEL68_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35241.1"
                     /translation="MRGKKAQLIAGLVLLTVGALFILDRLIILEIYISWPIILVAVGI
                     ALFLINPQSLASWIVGGIGVILFIVNFVIAFFPEVEAWSDLIWPTILVIIGVLLLYRY
                     YQTPSPKPPAQPPPSKQEEQGTNPEEPPKRAES"
     gene            27287..30244
                     /locus_tag="VEL68_04390"
     CDS             27287..30244
                     /locus_tag="VEL68_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXZ35242.1"
                     /translation="MISFIEKIKRHRFYAPQVVYHQPFPSERANFSRPGKDFSPGLAR
                     ALEKMGIGRLYSHQVEAIEQVGRGKNVVIATPTASGKTLIYNLPVVERLFQNSEDKAL
                     YLFPLKALEQDQLKAFQEFVQPFQAPVPFKAEIYDGDTSSYRRKKIRESIPPVLISNP
                     DMVHLSLLPFHAQWETFFRNLRFVVIDELHTYKGIFGSHLTQVLRRLERVCDFYGSTP
                     QIIACSATIANPQAFAEKLTGLPFEAVTQSGAPRSGRHFLFLNPNTSPYTLAAKLFLD
                     CVDGGFRTLAFTQARKITELLHTWVLKDRPDLRGKVSSYRAGFLPEERREIETKLVSG
                     ELHGVISTSALELGIDIGGLDVCILVGYPGTVAATWQRGGRVGRADRESLIALIAQPD
                     ALDQYFMHHPQDFFGRGFESAVVDPDNSVVVSKHLVCASAEIPLRTDESIIPVKNYAP
                     LLDQLVSDGELLKSASGREWFSHRLNPHRLVDIRSAGEGFTIFEEESKRLIGQISVPR
                     VYSECHPGAIYLHRAEPYLVTHLDQGKREVWAKQVEVDYYTRALSEKETEILSTVRTQ
                     AFAHFTACYGRLKVTERVRGFEKRRIFGQDLLSVHELEMPSHIFETMGLWIILPGEMP
                     QRFKDQGLHFMGGIHAVEHASIALFPLFALCDRNDVGGICYPNHPQLEKPAIFIYDGY
                     PGGVGLADYGYGVIEELLRKSLGMIRDCPCVDGCPSCVHSPKCGSGNKPLDKRAAEFV
                     LEWLLGESKIEKKGRGKGRVAVKDEVFPTPSDIFAGDSELREPESVACAPAPAWLKEE
                     LARHRILFLDIETQRGAEEVGGWHNKHLMRVGAAVVYDSLEDVFLIFEENCVHELIER
                     LKTAELIVGFNIVDFDYQVLKAYTSLRFQQLKTFDILQEVSRQLGYRLSLGHLAHKTL
                     NTEKSADGLQSLQWFKEGRIEEVIAYCKKDVEITKDLFLFGLANGYLLFETRAGQRVR
                     LPVEWKLERILKE"
     gene            complement(30338..30757)
                     /locus_tag="VEL68_04395"
     CDS             complement(30338..30757)
                     /locus_tag="VEL68_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXZ35243.1"
                     /translation="MFKKILVPLDGSKLAEGIVPRVEWLAKVHDGEVTLIRVALAHTF
                     PGMDPVQHQVNVVREAEEYLAKVENNLRSVGVRVKSVVRYGNDAQEIVDHARDRDFDL
                     IAMSTHGRTGLSQFVLGSVANKIIHTATVPILLCRVC"
     gene            complement(30924..32879)
                     /locus_tag="VEL68_04400"
     CDS             complement(30924..32879)
                     /locus_tag="VEL68_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HXZ35244.1"
                     /translation="MAEEKKTAPVAEEDFITEERLKKAEAYIEEEEGPSRRLVGKMGV
                     FITVVAVIMSLIHLYAAVGIIMTQILRGIHVMFVLFLTFLVFPSLKRYRNQIFWYDYL
                     LSILAIACIVYTFLDFEEFIYRSVTPTYGDLFFGSLLIALVLEANRRTTNWILPLVAG
                     SFLIYAYVGPWLPPPWTHRGYGLDRLVGHMYMTLEGIFGVPIDVSSTFIILFTIYGAF
                     LEFSGAGKFFIDFSLTAMGGKPTTAGRTVTLASFLLGGPSGSGVATTVTLGAVAWPML
                     AKAGYNKEAAGGLLSAGGIGAILSPPVLGAAAFLIAEFLKISYLDVIVMASIPTILYY
                     WAIFLMVEFDAKKFGAKSVAFDHTYSLGQLTRLYGFHFTSLISIVVIMVLGFTPILAV
                     FWSTVIAFAVSFIRRDTALVPRKLIAALRGGSIGVLSVATTCASAGIIVGVTTLTGLG
                     LKFSSIVIEYAGGNLLLTAIFTAFVVWVVGLAVPVTASYIICAVIAAPALIKLGVPDF
                     AAHMFIFYYSILSEVSPPTALSPFAAAALTGGDPYKTTMQAWKYTLPAFLVPFNFTLH
                     PDGVALLLKGSPAGIIWTFVTALVGIFALAAGMDGWMFKKATWYEKIILIIAGLALVY
                     PSWVYDVIGLALVGFAVFSQKVLRKND"
     gene            complement(32879..33373)
                     /locus_tag="VEL68_04405"
     CDS             complement(32879..33373)
                     /locus_tag="VEL68_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35245.1"
                     /translation="MKIKGWLPLLALLTLAAAGGALFIFEVPVLQVVNAERPARLNLR
                     LGEPTRFSISYIHSIYLQAAAEEFEVEEGEVILLRGVRTRSPSVAHYYGFEEDNRDYY
                     PVDRKMKSFALRLGMSQAQTLSHGEKKISLGDLGEKGDRLEVRVVRMTWGKYLLSRVV
                     KEKS"
     gene            complement(33374..34357)
                     /locus_tag="VEL68_04410"
     CDS             complement(33374..34357)
                     /locus_tag="VEL68_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ35246.1"
                     /translation="MMGKGKYGLAFLILFVFLFSSENGWAQSKEMNRLSLLTGGTGGV
                     YYPLGGALAQVISKYVPNTSSTAEVTAAAVDNIKLLKAGKGELALVIADVANDAILGK
                     ERFEKDGKIPLRTLAVLYGNLNHMVVLKDSGIKSVPELKGKKVSVGAPGSATEVKSSR
                     ILEAFGVDPAKEIRRERLSVAESAGALKDRKIDAFCWDGGLPTAAVMDLAATPGISAR
                     ILPQDKALPTLNRKYGPVYYNAVIPKGTYAGMEMDVPVIGVATMLVCMESFDPGLAYA
                     IVKAILEHKSDLVAVHKTAEHLSLQGAVTGSSIPFHPGALKYYAEKGIKVP"
     gene            complement(34568..35551)
                     /locus_tag="VEL68_04415"
     CDS             complement(34568..35551)
                     /locus_tag="VEL68_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ35247.1"
                     /translation="MATKTGGVFSLIFIFTGFIVFSGAAGAQTKANRLSLATGGTGGV
                     YYPLGGGMANVISKYVPNTEATAEVTSASVDNCKLIHAGKADLALIMADTGYDADKGL
                     ERFKTTGPQAIRTVAVVYPNYMHFVTVDGLGIKSITDLKGKRVSTGSPGSGTEVMALR
                     VLESYGINPDKDIKKERLGVAESAGALKDRKVDAFGWVGGLPTAAVMDLAATPGVKIR
                     ILNNAEHLDRMIQKYGPIYFKRDIPRNTYTGMDADVPVACVANLLICREKMEESLVYN
                     ILKVLLEHQPELVAVHKEASNFTLASATVGSSLPFHPGAIKYYREKGIKVQ"
     gene            complement(35729..36922)
                     /locus_tag="VEL68_04420"
     CDS             complement(35729..36922)
                     /locus_tag="VEL68_04420"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ35248.1"
                     /translation="MPQALADLKILDLSRVLAMPYCSMMLGDLGAEIIRVERPGVGDE
                     TRHWGPPWVGELSAYYLCTNRNKKCITVDLKKKEGQEIIRRLAQRSDILLENFLPGDL
                     AKMNLGYEDVRAINPKIIYASVTGYGQNGPYRDLPGFDFILQAQGGLMSIIGEEEGSP
                     MKVGVAIVDITAGLFACSAILAALHYREKTGEGQYIDMALLDAQVAWLANQASNFLVS
                     GKVPRRMGNAHPNIVPYETFKAKDGIYLALGVGNDNQWKKFCKIARIEHLMDDPQYAT
                     NPKRVENRKKLVPLLQEVFLQRDSGEWLKLLGEAEIPIGPINTIDRVFADPQVLAREM
                     LVEMGHPKVGKFKLVGSPMKLSKTPVQYRIPPPLLGEHTESVLHEVLGYDKEKIARLK
                     EGKVI"
     gene            complement(36976..38541)
                     /locus_tag="VEL68_04425"
     CDS             complement(36976..38541)
                     /locus_tag="VEL68_04425"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXZ35249.1"
                     /translation="MEYKEKGFEALYKDYQERATKIKQMGGPQAIAGQHKEGKMSARE
                     RVEYFFDPGTFTELGMFVRNRTTAFGMDKREVPAEGVVTGFGKVNGRMVVVGAEDYTA
                     MAGTFGEYHGKKFSQAIDFAKDKGWPFVGMNDSGGARLQEGMDTLQAYAWLFRSQILA
                     SGIVPQIALLMGPCLGGQAYHPIMQDFIFQTRKTGFMGIAGPAFVKTQTGVEISLEEL
                     CGVKAHAYKTGQTHYVAEDDKDCLDRAKELLAFLPSNNRERPPRKATSDDPDRPTPEL
                     DTLIPETPSKPFDMHKVIARIVDDGSFFETQKNHATNVIVGFARFHGRPAGIVASQPM
                     SKAGAIDCDAADKVARFVRFCDLFGIPLVNLHDTPGYWIGPEQEWKGILRHGAKMLYA
                     YIDATVPKISVIVRKSYAGAYLGMCCKDTGADFMFAWPQAKVTIVGADTAASVIFAKE
                     IKSSPNPEETRRQRIQLYSDLYENPYRGAERNYLDDVIMPRDTRKIINRCLDVLENKS
                     EARPWRKYSNINL"
     gene            complement(38560..39717)
                     /gene="acd"
                     /locus_tag="VEL68_04430"
     CDS             complement(38560..39717)
                     /gene="acd"
                     /locus_tag="VEL68_04430"
                     /EC_number="1.3.99.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaryl-CoA dehydrogenase Acd"
                     /protein_id="HXZ35250.1"
                     /translation="MDFKLSEELQAIRETARDFAEKEIIPFADKWENENYFPREVIRK
                     MGDLGFYGTLVPEKYGGNGMGFLAGTIITEEIARASASLRVTFNMQTFGPALSIYRYG
                     TEEQKKKYIPKLVSTEFISCFAITEPNAGSDVMSMKTTAIPKGDFFLVNGSKTWISNA
                     QVADLCILYAYHDPAAKSRGLSTFLLDMHQPGVSTRPLDKLGLHSAPTGEIILEDVKV
                     PKEAVLGELGEGVKHVFGSLNQTRLSCAAGGVGIAQACLEAAVKYCNERSQFGQQIGQ
                     YQMNQDMIAIMATEIEAARFLVYRAALQKDEGMLNNVLETSMAKYYAGEVAGRAADFA
                     MRILSAYGYSTEYPVARYYRDAKSISIVEGTANIQKTIIAMDQLGYRKANR"
     gene            complement(39859..40074)
                     /locus_tag="VEL68_04435"
     CDS             complement(39859..40074)
                     /locus_tag="VEL68_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXZ35251.1"
                     /translation="MAHNVTAPMVGKIVDIKVKVGDQVKEDDEIAVLEAMKMEMPIVA
                     PASGVVKEIKVSVGQTCETDMVLAVIE"
     gene            40451..41830
                     /locus_tag="VEL68_04440"
     CDS             40451..41830
                     /locus_tag="VEL68_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXZ35252.1"
                     /translation="MMRSQGFQKFARRLNLWVAYFVPHSFLFVILLTFLVYFLGILVT
                     YQGPFDMVQFWVRGVWNLLSFSMQMVLLVAAGFSVASAPLSQRVLRFLAALPSSPRGA
                     VLFLTAFTALCSLLHWGLGIIAGSFLAREMGRRVSGIDYPLLVASAFIGMASGNLGLF
                     ASDPVVIRKAAPFLEKAMGSSPTPETAWSLVSLLGFILGLAAVMAMLGVLSPEEKEAT
                     PPGRELLERFEREDRAELGTIAAEREVHQKGKLAFGVWLEHSRWPVWLISIMGFSYIV
                     FWFYGQGFAFNLNTLIFVLFTLALSLHDTPMHFLQSLENSARAAHGIIVQFPFYAGTQ
                     GMLASSGLAALATAWVLWLIGPATYPTLIFLHAVVLNLFAPTSGGVWEVQGPLMAKAA
                     QSLELHFPRVIQAFSSGEMLGNVIHPFWTIPLMGICGLSVRDIMGYCLIAFVLLSAIW
                     VLCVNLLPV"
     gene            41918..42958
                     /locus_tag="VEL68_04445"
     CDS             41918..42958
                     /locus_tag="VEL68_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ35253.1"
                     /translation="MPEITSRDRIRAAMKRTYADRIPYSLTLGPFRARVLGCSPRQYY
                     QEGSKLAHSTLICHEMFQHDSVEVGWDIFLEAEAAGAELEFPDDGVPQVRKHALSRKA
                     AISALQIPRPESSGRYPLYLEACRTVASSLREVAMSGTVTGPWTIAAGLRGTQELIYD
                     TVDDPGFVGELLKFTTEVTQILGAGVVETGLGLTMGEAASSCSLISPAIYRRFIKPCH
                     QNIVQFFREKKINLSLHICGHIDPLMEDLMDLGIVALSMDSPSSLKRMVEVSQKRILL
                     LGNVATSLFVSGSREEIENSVKECIRTAAPGGAFILSSGCELPYNATVERARFFIAAA
                     RKYGRPEWFLAK"
     gene            43219..45000
                     /locus_tag="VEL68_04450"
     CDS             43219..45000
                     /locus_tag="VEL68_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ35254.1"
                     /translation="MYTWFYGFSEEPFNVNPDPVFLYLTKTHQQALDDMVRALQERKG
                     FALILGDLGSGKTTLIQYFLNTVDPKTLVKAIFHPPGTIEDLLEEVLRNLGVPAASHD
                     KDSLVGQLNDFLHRLSPEETLALVIDEAHEMSSKVIEDLRLLPTPENISSRKLQVLLV
                     GQPELKDVLKSERLKDFLETIEVQGELHPLSEEESRLYISHRLQKADSEISRVFTPDA
                     LDLICQHAKGNPRKLNILCDNSFLIGYGLSKKKVDSAIVSEVLEDLDFAGEEERIAWK
                     SEESLKPKTLAYRIKNPLFRKLAYSLLALCGVGAIILLGRMYLKSPEETVSSRFPIKP
                     PVVKEIRPPEAKPDLAAKSPPQPEAEKKPSAGVPEPSSPAIAEKKPSTPPSGGETKSP
                     VPLPVAETGPAPRKPESPPVAGEPKPSAKAEPKFAVTKPIPVQASGPGAKEEAVAKKT
                     VLVRGKDTLYSIALRNYGVANTSIVDQILEANPKIGDPNKLSDRQSVRLPEVHEESLL
                     IPSADGTCQVRLGTFLKAEYAAFLKGEPSLRGKTVEVIPRKLPSGETWYRAVAGQFNT
                     REEGLKVIQELKRKGLSPYFPGFRKKK"
     gene            46127..46816
                     /locus_tag="VEL68_04455"
     CDS             46127..46816
                     /locus_tag="VEL68_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ35255.1"
                     /translation="MNHLREVVRGVYLVGSPELTAPEDCCVYLLDLGDLVLIDAGAGR
                     TVPAILENIASLGLHHKTLAAVVATHCHIDHIGGIPEIRQRTGCQIIAHSLDAPAMEK
                     GDSGKTAADWYRMTFPPTPVDIKLNEVREVLKFEKGEVLCLHTPGHTPGSISIILDRE
                     GQRVLFGQDIHGPFSDSFGSDLAAWRKSMGILLGLKADILCEGHFGIVQPEKEVRRYI
                     QDYLERYKKEN"
     gene            47005..47805
                     /locus_tag="VEL68_04460"
     CDS             47005..47805
                     /locus_tag="VEL68_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HXZ35256.1"
                     /translation="MSKNRSGIVTLITDFGWGGEYVGAMKGAILRVNPECRVVDLTHQ
                     VEPQNILQAAYILKNTFPFFPPGTVHLAVVDPGVGSRRKAIVVKREEHLFVSPDNGVL
                     SGILGGKETASGYEIKERKYFLSPVSDTFHGRDIFAPVAGHLSLGLDPSKLGPQIQGL
                     KTIVLPRPRMVKGGLRARILWADSFGNLITNLAREDYGRKLETRPFQIKGKGWKIDRV
                     YRTYGEGLPGQPMALFGSGGFLEISVNRGNARKTLGLKPGDPIIILWR"
     gene            47796..50054
                     /locus_tag="VEL68_04465"
     CDS             47796..50054
                     /locus_tag="VEL68_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXZ35257.1"
                     /translation="MAIRILRDNPRLGIPSFFVLITLLFPGGDLPAAPSLPGQFEQGV
                     LLFKKGEYSEALKIFQGLEEASKEPSSTADLAFMQGQVFRELQNWPEAAQAFSRAAEN
                     HPLLADYALFFQGESLQKMSEGEKSLAVFRRLFDLYPQSLVNTQARLRTAEIYFQSGD
                     FQNTAEVCELILQGSPWKDIPAQAWFLLGQAREGLNQYSEAIQAYQETWMKYPLHGAA
                     KKARTRWEALAQEKKVAVKKISTEALFNRALQFYQAHQYETALKEMNRIEGLPAQSYP
                     ATYAGELWVDDLYYHRGMSYFRLKQYSKAVENLDLVVRSSRNEGMAEKSLFSLFQTLT
                     RSGRREEALRIHDLFQASYPRSSFLPQALYLKASLYEDRGEPEKAVSLYLSVADQFLQ
                     SPFRFPALWNAGWVQYQKKDWAAALQAWERLKKAEPHFRWMEKALYWSGKALEKIDRK
                     EEAEEQYQQLLREYPASYYSQLISSRGLEPRASPGPVPILQDRVLSSVEEMKTNSPGP
                     KSIHLEKGRLLARLALSPWAIDELEAAEGEGGSLDEKWIEVSRLYRESEEYYRSNLLV
                     RKKFALKPFSGSSSEKERTLYLLAYPLGNSSLVNRFSQACNLDPALLSALILEESRFQ
                     VQAVSPAGARGLMQVMPQTGRQIAKELKVRPFSEDQLFDPAVNIRLGSRYFAKMLEEF
                     EGKVHLALAAYNAGPQAVRKWLASSLSSAEDEFVENIPYLETRNYVIRVIGSAQVYRL
                     LYCPQPDSPAQP"
     gene            complement(50018..51457)
                     /locus_tag="VEL68_04470"
     CDS             complement(50018..51457)
                     /locus_tag="VEL68_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ35258.1"
                     /translation="MKILFMQPSQERGLGFRSLAVVEPLGLESIAANLPEHNVKILDL
                     RIENRLPRVLEEFQPDFCGINCSFTIDVYQAREIATTIKKWNPRIPVFVGGHHASLSP
                     EDFYCSDIYGVVVGEGEAAIRDLVRACEQGRDLKTLPGLVINEPSAPFYTPERPMVEN
                     LDEIPIPARNLTRHYRKHYYLGFQKPFSIMETARGCAYRCDFCSVWKFFNGKCRMKSP
                     ERVLEEVKTIPEKYIFLADDNFLQSVPRAKRIAQLLIDSRIKKRFTFQARSDAIVRHP
                     EVIPLLRKAGFWKVFIGFEKIDEEAMSQLNKHNTVQNNEEALKVLRAHGMEVIAAFII
                     DPSFQKQDFQRLRQYILDRKLYSPSLTILTPLPGTDLFARVKEKLVTTNYELFDYVHA
                     VLPTKLKLADFYREFTELYKTGYAWSQIGWEGAAAILRHTFSISHLISMKRAAWDSVN
                     PINYLAGHEREAVPLKVNQGWAGLSGCGQ"
     gene            complement(51532..51609)
                     /locus_tag="VEL68_04475"
     tRNA            complement(51532..51609)
                     /locus_tag="VEL68_04475"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51572..51574),aa:Asp,seq:gtc)
BASE COUNT        11631 a        14285 c        14657 g        11417 t
ORIGIN      
        1 cctaaccagc cgggagcgga tttggatggc ggcgtttaaa gatatctgct gctccgaaag
       61 ggcaataacg gccatttcaa agacggtgga agggcggatg ggcttcaccc cataaagggc
      121 cttgaccagg gggcggagga aggggtgctt tcgggcccgc tggtaaaagc cttccagaga
      181 agcttcgaca tggaattgcc atcgaaccct ttctttcagg agagagacct ttcggacttg
      241 ttccgggaag gttcggacga tgaggcggac tcggtcattc ttcgagccga attcctgctg
      301 aaccctggtc agaatgggct caccttccac aacatgaagg cggtggtatt ccgccggcag
      361 agtctcccga gggggaatcc agacatcggt cccgtcctga ggaaaatgac aaaatgccca
      421 tgcgcagcgg gtgagatcgt agggcccttc catgaggagc tcaaaattca ttttcccccc
      481 tgttcggttt ccttgaattc cctcggctct tctcaaaata ctttatggca gccggaaagg
      541 gaagagaaag tacgaaggag agaagatttt tggctcgaaa gctccccttc gggcatataa
      601 aaatttcttg ttaaaaatat ttattttggc tatgttaaac attgatgaac tcgtaaaaag
      661 tcaaaattca attaaattcg tcattccggc ggaagccgga ctagttattt gaataagtta
      721 tggaccccgg ctttcgccgg ggtgacgctt aacgagactt tatacgagat catcaaacat
      781 agtttaatca aagaatatca ggaggagttt caaatgagac cctatttcga aaaaatgccc
      841 gctttcggca aacctttgtc ggaaaggcaa atgcagagtt ctgaagagaa catcaaaaaa
      901 attcgggagg tggaacagga gatcgccgaa gccgtaaaaa aggtaaaagg ggccggcatc
      961 tccacggagg agatcaataa acggggggag atgaccgttt accagaggct ggaatatttg
     1021 gttgaccccg gcacctggtg tcccttgcac accctctatg acccgatgga ggaggagtcc
     1081 ggaaccaccg gggtggtgga cgggttgggc aaaatcggcg ggaaatgggc ggtgatcatc
     1141 gggtttgaca acaaagtgat ggccggagct tggattgccg gacaatcgga caacgttctt
     1201 agggtcaccg acatggccaa gaacctacac cttcccctgg tctggctggt gaactgcagc
     1261 ggggtgaagc tgccggagca ggagaaggtc tacgccaacc ggaggggaag cgggaccacc
     1321 ttcttccgcc atgccgaact ggaaaaactc gggattccag tcctcgccgg aatctacggg
     1381 accaatcccg cggggggagg ataccaggga atcagcccga ccatcctcct cgcccacaag
     1441 aactgcaata tcgccgtggg cggcggagga atcgtgagcg ggatgagccc caagggcttc
     1501 ttcgatgaag aaggagccga ggtggtcatc gaagccaccc gaaagttcaa ggccgttccc
     1561 cccggccggg tggaaattca ttacgactcc acgggatttt tcaaggccgt ctttcagaag
     1621 gaagaagggg tgctggacgg gttgaaggaa tacatgaaat accttcccgc ctacgacccc
     1681 aaattcttcc gtgtggccga acccaaagcc ccgaagtttt cgcccgaaga gatcaatcgc
     1741 ctggtcccct tcaaccagaa gatggcctac aatttcgagg aagttctggc gaggctgacc
     1801 gacaacagcg aacacatgga gtttcgaccc acctacggcc ccgaggtcta caccgggttg
     1861 gccaaagtgg acggtttagt ggtgggcatc atcggcaaca aacagggaat cctccccaag
     1921 ggatacccgg aatacgctcc ctatcctggg gtggggggga aattctaccg ccaggggctc
     1981 atcaagatga acgagtttgt aacccagtgc ggaagagacc gagttcccat tatctggttc
     2041 caggacacca ccggcatcga tgtgggggac ctcgccgaga aggcggaact gctggggttg
     2101 gggcaatccc tgatttactc catcgagcag tccaacgtac ccatgatgtg cgtgatcctg
     2161 agaaaaggaa ccgccgccgc ccactacatc atgggagggc cccaggcgaa caacaacaat
     2221 attttcaccc tcgggacgcc taccagcgag atttatgtga tgcacgggga gacggccgca
     2281 gttgcctcct tcgcccggag gctggtgaag gagaaggatt cgaaccggcc cctgaaaccg
     2341 atcatcgaca aaatgaacgc tctggcgcag gactattaca acaagtcccg gcccatctat
     2401 tgcgccaaga ccgggttcgt cgatgaagtg gttcccctgc cggaaatccg gaagtacatg
     2461 gtggccttta cgggcgcggc ttaccagaat cccaggtcca tctgtcccca ccaccacctg
     2521 atgcttcccc ggctgatcaa aggatgaacc aaaggcccgc cttcgagcgg gccttttctt
     2581 ttcagcttca aggcctattc cgtctatacc acaactgttt ttccccctat tccctgagag
     2641 attccgacgg ccaggtattc caggtcttcc cctccgccat ttttcatttg gtggatttct
     2701 ctcgggagaa taatcactgc ttccccttcc cccatttcag cttcttcccc attgacgaga
     2761 attttggctt gccccttcaa gatgatgaag atttcctcca tgtcctcgtg ataatgggcc
     2821 tgaaaggctt tctccggctc catccggcac cagttaacca tctgcactcg tccctccagc
     2881 aaatcgtctt tttgaagcaa gacctttttc cagacggaag gggattgcag gctttcgtga
     2941 ctggccggaa tccaatccaa atctttaagt cggaaaattt tcatggcata ttccggttca
     3001 tttgaaatcg gtgcagctgt attactaaag attccggggt tgagaagaca tattcttccg
     3061 aaaatctcct ctaaccccga tgctcttcac ttggcctagc accgttcacc ctgagcttgt
     3121 cgaagggtgc gtcgctaagc cgttcatgct tcgacaagct cagcacgaac ggcttagtct
     3181 tcagcgtcca tacaattata gtatgatcaa tagtttactc tgaaggaatc atgattggca
     3241 attccagatt gtgcctttta tccccccctt tatccttccg gattccgcat ttggtcagtc
     3301 ttcttttagt cttgacaatt ccgaaagggg ggagatattt ttgaatggtt caaagcggcc
     3361 agggctgaaa ttctgatttg cagcatcagc tctgagagac cggcattttt tcttcatttg
     3421 ccggtattag actctacaag gaggtgcaaa gatgaagaag atgttgggtt tgactttcat
     3481 cctgtttttg gcctttggct ccctgggctg gacccaggac acgatcaaga tcggtgtggt
     3541 cgggccgagg accggcccgg ccgcggccac tggaaaggcc tttgaggaag ggattgccat
     3601 cgccctggac cacatcaacg ccaaaggcgg agtgctgggg aagaaggtcg aggtaatctt
     3661 cgaagacacc ggcggagttc ccgaaaaggc tgcttccgcc ctggagaggc tggcgacccg
     3721 ggagaaggtt ccagtggttg tcggggagag ccactcctct tccgccctgg cagagatcga
     3781 agtggccaac cgttataaga tccccctcat catctgcgaa gcctggcacg acgacatcac
     3841 caagaagggc tacaagtggg ttttccgggc cggcccggcc aacagcggcg tggtcgattt
     3901 ctacatcgcc cccttcgtga aggagaacgg gttcaagaaa gcggccatcg tccgagagaa
     3961 ctccgactgg ggcgccggca tcgccgagcg gacggagatg aacctcaaaa gcatgaacat
     4021 cccctttacc accctgaaag tggagcggga gagcaaggac tactacaccg agctaaccaa
     4081 gctcaagagc gagaacccgg acatcatcct ggcctacatc tacgggaccg gcctgcactt
     4141 cttcatcagc caggccagcg aggtggggct cagccccaag gccctcatcc tggacggggc
     4201 cggacccccc agcctctggg acagctactg gaagaatgtg ggcgaggccg gggagctgga
     4261 actcttcatg tccagcatgc acgaaaaggt ccacctgacc gctaccagtc ggaagttctg
     4321 ggatgactac aagaagaagt acgggaagga cccctcggac tacaagaccc ggtccatcta
     4381 caacgtaatt ctcatcgccg ccgatgccat caaccgggcc aaatcgacgg accccgaagc
     4441 cctcgtcgca gccctggaga agaccaacct cgaagtcacc accgggacgg tcaagttcgg
     4501 tatgaaggag ggatcgtacg agtaccatca gtggatgccc cccatgctgg tcgttcagtg
     4561 gcagaacaaa cagcaggtgg tggtctaccc caaagaggcg gccacgggtg ccttgaagaa
     4621 aacaaagtaa gaaagcagtg tcagtgtcag tgtgagtgta agtgaaagga tttgactatt
     4681 tttactgaca cttaccgctg acactgacac ttaggttttt taccatggct gaattcctga
     4741 tgtatctaac caacggaatc gttatcgggg tgatcaacgc catctcggcc atcggcgtgt
     4801 ccctcatcgt gggcatcatg aaggtggtga atttcgccca cggggagatg tacatctttg
     4861 ccgggtactt cagttaccac ttgagcacct ccctgggcct gccccccgtc ctttccatga
     4921 tcgtagccgt cgggttcgtg tttctcttcg gactcgcgat ggagcgcacg ctcatccgcc
     4981 ccacttacgg ggatgacatg tattccctga ttatcacctt cgtgctgtcg atcgtccttc
     5041 agaacctggc gctcctgatc ttcggcccat acccgaacaa accccccaac ctggtgagcg
     5101 gggccacgaa cctcttcggc cttttccaat atggaaacca gcggctggtc tctttcttca
     5161 tttccatcgg catactgatc ttcatttttt atctcattaa aaaaacatgg tttggaaaga
     5221 gcatccgggc catttcccag gaccgggaaa tgtcttccct catgggagtg gaccatctgc
     5281 ggatgaacat gttttccttt gggctggggg ccgccctcgc tggggccgca ggagtcatcc
     5341 tggcccccgt ctttccggtt accccgaccg ggagttcctc gatcagtttg accgcctttg
     5401 tagtggtcgt tctgggggga atgggttccc tcaaggggtg cctggtggga ggattgatcc
     5461 tggggatcat cgaaaatctg ggcgctgcct acatctccac catgtaccgc catatttttg
     5521 ccttcgtcat cctgatcatg gtccttgttt tcaggccatg ggggctgtat gggcaaaaga
     5581 cctaaaaagg ggtgcagagt gtaggggcga ttctcgtaga gtcgagtctc cgacatgaat
     5641 cgcccctaca gggtccgtaa agcaaccagc gccgagaaac gaacaacgag taacgattca
     5701 aatatggact tgaaaaaact ttggtggatt ccgattctgg cctttcttgc gatcctgccg
     5761 ccttttgtcc tatcccagta ctggctccac acgatgatca tcagcctctt ttacgtgatg
     5821 atggctgctt cgtggaacct tctggccggc tataccgggc aggtttcctt tgcccatgcg
     5881 gctttttgcg gggtgggcgc ctatgtctcg ggaatgctgg ccgtgttcct gggagtgtcc
     5941 ccgtggctgg gaatcctttc cggaacggcg atggccgcgc tactgggatt tggcctggga
     6001 tttctctgca tccgcatggg gggaatttac ctttccctga cgaccctcgg gttttccgag
     6061 atcctgcgga tcatcatcac caacgaggaa aaatggaccc ggggaaccat gggcctgcag
     6121 acccccggcc tgtttgccaa gtattccaaa acggactatt acttcgtctt tctcgtcgcc
     6181 accgtggtga tcctgatctt cattcaccgg ctgatccggt cggagtgggg actcaccttt
     6241 cgggccgtgc tgaacgacga agtggccgct tcctcctcgg ggatcaacac catccgggtc
     6301 cgggtcctgg ccttcaccct gaccagcggc atggccggac tggccggcgg gctctacggc
     6361 cattacctca tgctcattac tcccgacatc ccctccctgg ggcagatgtt cctggttctg
     6421 gccatggcgg tcatcggcgg aatgggcagc tttgccgggc cggtcatcgg gtctttcgtc
     6481 ctggaaatcc tttccgaata catccgggtg tacggcgagt accatgtcct gctcttcggc
     6541 ctggtggctt tggcggtagc caggtttgcc cccgaaggaa tcatgggaac ctgggcaaaa
     6601 atgcggagat cgccgtgagc gccattctgg aaggaaagaa tttgaccaaa tctttcggcg
     6661 ggctgacggc cgtgcgggag gtggacttcc acctagaaca aggtgaaatc gtaggcctca
     6721 ttggccctaa cggagccggc aagaccaccc ttttcaacct catcagcggg gtattcccca
     6781 ccgatacggg gaccctccgg tttaacgacc gggagatcgg ggggctgagg tccttcgaaa
     6841 tctgccggct gggaatcgcc cggaccttcc agatcgtccg tccttttttg aagatgaact
     6901 gcctggagaa tgttctggtg gggattgcgg gacgggagga gaaagaaacc cgaagaaagg
     6961 aagagaaaag agaggaggca tgggaacttt tgaagttcat cgggctgccg ggggaagaat
     7021 accggctggc ccagaacctg accctcatgc agaagaagag gctggagatg gcccgggccc
     7081 tggccacccg gccccgggtc attctcctcg atgaagtgct ggccgggctc aatccttcgg
     7141 aaatgcatca ggcgctggat ttgatcgagg ggatccgcag cgagcagaag atcaccatct
     7201 tctggatcga gcacgtgatg ggggccatca tgaaaggttc ggaccggatc atcgtcctgg
     7261 atcagggcag gatgattcgc gaagggaaac ccgaagaggt ggtctccgac ccaagagtca
     7321 tcgaggctta tttgggggat gcgaatgctt gaggtgaagg gaatcagcgt attccgcggc
     7381 caagcccaaa tcctctgggg catctccatg caggtggaaa aaggagaggt ggtcaccctc
     7441 ctgggggcca acggggcggg aaagacgact ttcgttgaat ccatcctggg aatccaccat
     7501 ccgcccagcg gtgccatagc ttttctgggg agcgacatca cccgcaggac cccgttccag
     7561 atcacccgcc tgggggtctc ctgcgtcccg gaggggcgaa ggttattcaa agacatgacg
     7621 gtctacgaaa acctggagat gggagcctat cctcccaggg cccggacggt cttaaggtcg
     7681 accgtccagt gggtcttctc cctcttccca gttctcgacc agaggaagat gcagattgcc
     7741 ggaaccctca gcggggggga acagcagatg ctggccattg ggcgagcgct gatgtccaaa
     7801 ccacagctgc tgctgatgga cgagctctcc cttgggcttt ctccaaagat caccaagggg
     7861 atctatggaa ccgtggacaa acttcgccgg gaaggggtca cccttttatt gatcgagcag
     7921 aacgccaccc tggctctgcg ccacagcgat cggggctaca ttctggagac gggaagaatc
     7981 accctccagg gaacttccca ggagctcctg gggaatgaac atatccgcaa agcctatctg
     8041 gggatgtaga agtagagatt cggaatcgga atatcgaaat tcgaaaaaaa gggcccttat
     8101 ggacaaaatg atcatcaccg tcggagtcac cgggtcgcgg atcagccgga agcaaacccc
     8161 ccacatcccc atccttccga aggagatcgc tcaatcgggc gtcgaagcgg tgaaggctgg
     8221 ggcctcgatc ctgcacattc atgtgcgcga tccccagacc gggctgggga cacaggacca
     8281 tgccatcttc caagaagtgg tggaccgggt ccgcagcgag gtggattgcg ttctctgcct
     8341 caccaccagc gggattccgg gacggaatct gcccatcgag caaaggctga ctcctctggc
     8401 cctgaaacca gagctggttt cctacgacgc cggctcgatc aacatggggg agaacgtttt
     8461 tctaaacccg ccggatttcc tggaagcact ggctaaagga tgcctggaaa gaggggttaa
     8521 accggaactc gaaatattcg aagtgggaat ggtctacacc tgcctcaagt actttgaaaa
     8581 aggcctcctc aagcctcccc tccatttcca gttcgtcctg gggacccccg ccggtatgcc
     8641 ggccacccct aaatccctga tttacctcac cgaaatcatc ccgtcggatt cgacttggtc
     8701 ggtcatcgga attggcccgg gccagcttcc cctggcagtc atggggatgg caatgggagg
     8761 tcacgtgcgg gtgggcctgg aagacaatat ctattattcc aaaggtcggc tggccaggag
     8821 caactccgaa ttagtggaaa gagtggtcaa gatcgcccgg gaatacggcc gggagatcgc
     8881 caccacccgg gaggcccgga agatcctgag cctggtctga tcccttcctg gataatccct
     8941 ccctacccac ttcgagcccg cgacagtgtg ctcaagaccc catcctcttt ttattgcgca
     9001 ttccaccctg caccgcgcgg ggtgcagggc aggcctgaac ctttcaaggt tcagggcgca
     9061 ctccggattc cgcattcgca ggtttgtaag gttgacaagc aagtcgtaaa tatgggaaac
     9121 taaaatccac cctccggctt catcaaccaa aaggaaaccc cgttttcctg gttcaccctg
     9181 aggaggaacc gatgaggatt accctccgca tcattctctc tatttttttc gtaatcagcg
     9241 tgattgtcct actgtttact ttcctgcagg tggagcagga aaaggagaga atgacccttg
     9301 atctggagcg acgggcctcc attctggggg agagcctgaa agaaagcatc gaacccctgg
     9361 tggccagacg ccagagttca tccgtacaga agatcgtgga caagttcggc aatcgggaaa
     9421 ggctggctgg agtagccatc tatgacgctc agggcaaact ttcagccgtc accgtccaat
     9481 tgacacccta tctcaagacc cctccatcca tcgtcgaaaa tgtcatgaaa gccgctgtgg
     9541 aagaggggaa cttcctcccc ctcggggaga aggaagcgta tctctacgct cttccccttt
     9601 tcaacgaaga aaaagtcgcc ggggcgatgg tgatggttta tgagaccaac caaatcaaag
     9661 atcgggtggc cctcatctgg cagaataatt tcatccgcct actggtgagc gccctcttca
     9721 tctctctcac cacccttctg gtgattcgct ggagcatggt ccacccgatt gcccggatgg
     9781 cggaatggat gaagaccctg agaaccggaa gggggggagc cccccttccg ctgcctccgg
     9841 ggaaagtctt tgaacccctg gcccgggaag ccacccggat ggtaaaaagc atctcggaag
     9901 cgcgttcggc cgccgagcag gaagcccggc tgcggcgcaa agcggagtct ctctggaccc
     9961 cggtgcggct ctgcgaacac gtgcgcaccc tcctgcaggg gaagcctctc ttcgtgattt
    10021 ccaaccggga gccctacgtt catgagagac ggggcaaaaa gattgagtgc gtcgtccccg
    10081 ccagcggcct ggtgacagct ctggagccga tccttcaggc ctgcggagga acctggttgg
    10141 cccatggaag cggagatgct gaccgggaag tggttgacga ccaggacaag gtccgagttc
    10201 ccccggaaga cccccagtac ctcctaaaga gggtgtggct taccaaggaa gaggaagagg
    10261 ggtattacta cgggttcgcc aatgaagggc tctggcctct gtgccacatc gcccacaccc
    10321 gcccgacctt ccgggccgag gattggaatc attatgaagc ggtcaattgg aagttcgccc
    10381 aggccacttt tgaggaattg gaaaaaaccc aggacccctg gattctggtc caggattacc
    10441 attttgccct tttcccgcga ataatcaaag aaaaaaggcc ggatgcccgg gtggccctct
    10501 tctggcacat cccctggccg aatcccgagt ccttcggcat ctgtccctgg cagaaagaaa
    10561 tcctccatgg catgctgggg gccgacctga ttggctttca cgttcaattt cattgcaaca
    10621 actttctcga aaccgtggac cgggcgttgg agtcgaggat cgactgggaa aatttcacag
    10681 cggaacggga gaaccatcta acccaggtga ggcctttccc catcagcatc gccgcaccca
    10741 atccctcttc ctctttccgc tcccggaatg gaggcctcaa aaaggccctt ctgcgggagt
    10801 tgggcataca agggaaatac cttggcgtgg gggtggatcg gatcgattac accaagggcc
    10861 tccgggagcg cttcttggcc atcgaaaggt tcctggaaaa atatccggtc taccaaggga
    10921 tgttcacctt tgtggaattg ggagctccca gccggaccca catcaaggaa taccgtgatt
    10981 tgaccgccca ggtggaatcg gaggccgaaa ggatcaactg gcgcttccag gccaaggatt
    11041 ggaggcccat cgtgttcctg aagcggcacc acagccacaa ggaaatcgcc ccattctacg
    11101 aggccgccga cctttgcctg gtaacttccc tgcatgacgg gatgaacctg gtggccaagg
    11161 agttcatcgg agccaaatgg gatgaatctg gggtcctgat cctcagccag ttcaccgggg
    11221 ctgcccgaga gctccgggac gctctggtgg tgaaccccta tgatacggag cagatggccg
    11281 aagccattcg cctggccctg gagatggacc ctgcggagaa gcgggcgcga atgcaccgga
    11341 tgagagaggc ggtgaaagaa aaaaacatct accgctgggc gggggatttg atttccagcc
    11401 tgagtcaaat ccgcctttct tcggttccgc ccctggggat cagcgcccca tataactcat
    11461 caaggccaaa taatctccca ggtgtcgggt gattaggaag tttcggcgga caaactccct
    11521 ggcgtcctgt cccatcctgg ccatcgcctc gggattgtcc agaaggtaat ggattcggaa
    11581 ggcggttccc tccacggaat tgaccgtgaa tccagtgacg ccataaacga gctggacggt
    11641 gatgcccccg gtaaaacctc caatcaccgg tttccccttc cacatggctt cggccaccgt
    11701 tagaccaaag ccctcccgaa gggatttctg caggacaatg gtcgcgaccc gctgaagagc
    11761 gttaatctcc agattagcat cagggggcag aacaagaacg cggatgtcgg gatcatcctg
    11821 ggcggcctgc cgcacttcgg ccaagacagc ctccccttca ggatcatccg tagctccccc
    11881 cccggccagg atcagctgca agtcatgatg ctttttgacc aggcggtaag cttgaatgac
    11941 ccccaggggg tctttgaagc ggtcgaagcg ggagacttgg agaaggatgg gtttatccag
    12001 agaaatcttc aaccgactta gaatttgatc cagctcctgc gggctgattc cccggttttt
    12061 atcgctcagg gggtcaatgg aagggtagat cagaaattga ggaatgggga gccgctgggc
    12121 aaaccggggg agggaaaaga tcgcggcatc atatttcacc acgaactgac gaaggaaatc
    12181 ccatactttc ctttggggct gagaaacatc gatgtggcat ctccagaccc atttcccctt
    12241 tttatttcgg cgactttcga tcaaggcggc cggctgagga tcatggatga tcacgaaatc
    12301 ggcttccagg gagaggcgtt tggcattctc gcgattgatc tccaggtaat tctggagcat
    12361 ttccggagaa atccgctgct cctgaccctg cagagcattg tgaaaggcct tggtcgtgga
    12421 aaagaattcc tcgtttccag taatcacatc ccactgagcc tctaatccga cgctctccag
    12481 cagagggacc aggctgcgta agatttccgc cacccctccg cccaatttgg tggagttcat
    12541 gtgcaggatc cttttcttcc cgatcttttc cgccatgcgg aggagaaact ccacgcttcc
    12601 ctggggagcg atctcccggt aggcctcgag ccggctcatt tccgatccct cccttcttgc
    12661 aggaattggt cacaaagatt caggattcgc gatcgagccg attccaggct gattcggtaa
    12721 ggatcgatat tggaaatcct cttgcccaat tccggcaggc ccaactgttc ttccatccac
    12781 agggcgaagt cccccttggg ttttccaagt cgaagaatgg cctcgaaggt gtgataataa
    12841 atgacgctgt catccaccgt ggccaggact tttcggaatt cggccaaagt atgcacctga
    12901 aggcccgtgg gagccgaaat gatacgggat tgcatgaagt gaaagggttc ttcataaatg
    12961 acccggggaa tgatttgcag cctggacagg tgatcggaaa tgatggttac gatttcggcc
    13021 cgaagggatt ccaggttttg aaaatcataa gggctgagga tgcccaattt ttctcccaag
    13081 acacgatccc gcacctgaat ggcggcccag gtggcaaaat cattgggata gggcccggca
    13141 atgtatttat ggcggaggag gaaactatgg gtatggtaat agatggaatc gaggggaact
    13201 tcttcgatgg cctccaggag ctgtcgctca tcacgggcgc tcctgccgag catctcgcgg
    13261 agctcccagc agcccacaaa gacaaaaggg tgttcagccg tacgactatc catgtttcaa
    13321 ttcctttctc ccggatgagg ttcggcctgg aatcaatttt ctaaaatttc ctgacagcgc
    13381 tggagaaatc ctctaacttc ctccggatcg cgcaggaaat actgggccga tgaagaggcc
    13441 gctccgggcc ccacccagac cgagattccc tgggaaccta taacccggaa agcgtcttca
    13501 tcggagcgat catcccccaa gtatacaggc aagggattaa ccgaaggaaa atgaatgaga
    13561 atctccttca cggttttccc cttatcaatg ccgactttgg gacgaatctc aacaatcttc
    13621 ttgccggggg aagactgcca ccggtccggg aaaggctgca agacctcctc cactgctttc
    13681 tggacccggg gggtgaattc ccggggtgta ttccggtaat ggacggtgag gataggccct
    13741 ttgtcttcga agagaatccc gggaatgagt tttaggttgg tttccagaga atccctgatt
    13801 tttctcagtt catcctgcct ttcggtggat aatttttttc tgtgggtccc ggcaggattt
    13861 tccacttcca ggccgtgatt tcccacataa atgatcccct gaactctcac tttatccttg
    13921 aggtcttcca gtgcccgccc gctaataatc gccaccctaa ccttggggga gtcccgcaat
    13981 ttctccaaaa ggaccttcac ctgggggggg cacacagcca agtccggacg tggcacaatg
    14041 gggcagagag tcccatcgta gtcgagaagc aaaaatagaa gacgggcgtt tcggattcga
    14101 ggttctacct catcccaggc ttcatacagg tgtttcataa attccccaat aatattctat
    14161 caatattttg agtattgttc acctccgatt ctcattccta tagttcgttg cggcgggaaa
    14221 aggatcggat caaaagatcc tgctttttta tcgggtggct ctgactctgg agcgggcatg
    14281 gttgtgatgc agaatccgca attaataaga gaacttgaca aaaagaggga tgatctatag
    14341 gatgagggta ttttaaatcc gggaaaaggg aaggggggat gaagatagag gattggggaa
    14401 aaatggtttt ccggagacaa cgcatcatgg caaggaaagg agaatagcat gaagcgcctt
    14461 cttatttatt ccctagtcgg agttatgatt atggtttttg ccgcggggac cgcactgggc
    14521 caggcccaaa aggaagcctt taaatacgag gacaaaatcg gggtcattaa gctgaaatcc
    14581 ggggagccaa tccacatcgc ctgctggatg gtgattgccg gtcccgacgc ctctttggga
    14641 acggatacca agcgtggagt ggaaattgcc atcgatgata agggcggaaa actcctcggc
    14701 catcctatca agctcaccgc tcaagacacg gggtgcaacg cggagggcgg acaggcggca
    14761 gccaccaaat tggccgccga ccccaccgtt gtggccgcgg tcggctccaa ctgctccagc
    14821 gaggcccggc cgggcgcccc gatcctctgg aaagcgggga tcgccacggt gtctccctcc
    14881 aatacggccc cctacctgac cgacccgaat cgggggccgg attatgacgg ctatctccgt
    14941 accgcccaca acgacaaggt ccagggagcg gtggccgcgg aatttgcctt caagcagctg
    15001 aaagttaaga aggccgcgac catccacgat gggagcgtgt acgccgaaca gctgcaggcc
    15061 gttttcgccg agaccttcaa gaaaatgggg ggtacgatca cctcccagga agctgtggcg
    15121 cccacggaca cggacatgcg gccggtgttg accaagatcg ccacaggcaa cccggaattt
    15181 ctatactttc ccatcttcat tgccgcgggc gggcacatca cccggcaggt gaaagaggta
    15241 aagggattgg agaaaaccgc cttgatggga gcggacggga ttttctccac cgacttttat
    15301 aaggctgcgg gagaaaccgc agtcggcatg taccactcca gccccgattt ttccgccttt
    15361 ggcgcaggct acaaagcctt cttggataag catcagaaaa aatacgggga aaaaccgatc
    15421 gctccgttcc acgcccacgg ctatgatgcg gccatgatga tctttgcggc catccagaag
    15481 gtggcgcaaa aagctccgga tggaactctg tacatcggcc ggcagaaact ccgcgatgct
    15541 ctgtatgcga ccaaaggatt gagagggctg accggaacca ttacctgcga caagtacgga
    15601 gactgcgcgg acccgagaat tgccgtttat aagacgacgg aagaaaacgt gaagaagctg
    15661 gtgatgcccg agaagccgtt ctgggcgccc gttgccgccg ctccagccgc aaagccgccg
    15721 gtccagaaag ccccggcgaa gaagtagccc aaggattcct tcgagccacg acgatgagcc
    15781 gggagggaag agaagcccgg ctcatctgct tggaatctat tttcatttcc gcaggcggga
    15841 aggggattca atctcaagca ccgatccccc gagacgcgtg accaaacctc aaatcaatcc
    15901 cggttatcca attcccaatg atccgaacag gatttggaga gttattcttg tggggggatg
    15961 ggtgcgtgga attcggtgct tgctccggtt cacctccccg gcattgggta ggaaaaaggg
    16021 ataggaactc aatttttcat aacgttcccg gaaattatta tgcggcagca atggagagaa
    16081 agaattagcc ttacggatgt cctcatgtgg ctgatcggcg gggccattct cctgctgatt
    16141 ctctggggat cgaccgccac cctggtttcc gggcggtaca gcgggcacca ttggtttgat
    16201 ttcatggtat tcgggctggc ccaagggagc atttacgccc tcatcgccct gggctacacg
    16261 atggtttacg gcaccctgcg gatgatcaac tttgcccaca gcgaagtctt catgggtggc
    16321 ccttacacgg cttacttcgt agccgcggcc ttttattcct ccggcttttt ggatcaacat
    16381 cccatcccca gcctgatcgt cgtttttttc gtttccatgg cgacttcaac ggccatcgcc
    16441 gtcctgctgg aacgggttgc ctaccgcccc ctacgaaatt ccccgcgcct ggttcccctg
    16501 atcaccgcga ttggagcctc cttctttctc cagtatacgt ttcgcgggct ctacggttcc
    16561 ggtttccaag cctacccggt ggtgaaggtc ctggaaggac aatggatgct cggagagttt
    16621 cggattctca aattccaggc cgtggtgatc atcgctgccg cgctcatgat ggccgctctt
    16681 tacgcgttcg tccagaagac caagatggga aaggccatcc gggcggtgtc cgaggacaag
    16741 gaagcggctt ccctcatggg aatcggcatc gaccggatga tcgtgaccac cttcgtcatc
    16801 ggggggattt ctgcgggagc ggcgggagtg ctctatgcgc tgatgttcaa acaggttcac
    16861 tttttcatgg gcttcatccc ggggatcaag gcctttaccg cggcggtcct gggagggatc
    16921 ggcaacatcc ccggggccat gctggggggg atcttcctgg gcctagtcga gtcggtgggc
    16981 cccagcctgt tcctggacgg cttgggcatc gtggcgccct atcagctcaa ggacgccatc
    17041 gccttcacaa tgctggtgct ggtgctcatt ttcaggccca ccggactgct gggagaaagg
    17101 ctggccgtga agaaggcata agaaatacca aatttcaaat ctcaagtccc caataaattc
    17161 caatttctca aattccaacg accaaagggg gtttagagat tggaatttgg attttcgagc
    17221 tgtcactcaa acagggaagc aaggagatga agaagggttt cggcctctgg cacaacggga
    17281 taaaggtcgg cctgatcatg ggcgccgtgg gggtcctgct ggcgctgatc ggaacagtgg
    17341 aagccttcag ccagcggagc gtgatcagtg aggtcatctc catgggccat acccttttac
    17401 ttctggttgg tttgttcggc ggctacctgg cggcccgccg caccacccaa gcggaatcgc
    17461 catacgccat gctcaacagc cttctctcgg gactggttgc cggggggatt ctttcccttc
    17521 tggttctcct gggaagcgtg gtaaatctgc gcaagatttt catcaatgcc tctcccctcc
    17581 tgtatcgact gctggccttt gacctagagc catggggcgg gagcattatt ctccttttgt
    17641 tcggggcgct gagtggcctg ttggccggcc ttttctatct ttcttctccc ctggctcgcc
    17701 gggtactggg aacctccctg ggctgcatgg tcatcaccgg agtattgcag gacttgctcc
    17761 gccccacctt cgccctctgg gggtccctcg ccatcatcaa tgactggctt tatgcggcca
    17821 acggcctgac tctgccagga gccgcatccc ttttcatctt gattgccctg gccactttcc
    17881 tgtgggccaa aagaggaggt gccctccagg cgggattcca gcggatgccc ccggaacggc
    17941 agcggttatt tcgccttgcc ggctggggtc tcctggccct ccttctattc ctgcttcccc
    18001 aggtcctcgg cctgttcttg agcgaggtat tgaccattgt gggcctctat gttcttctcg
    18061 gcctggggct gaacatcgtc gtcggctatg ccggcctcct ggacctcggg tacgtggcct
    18121 ttttcgccat cgggtcttac acgatggcgc tgctcacctc ccccgagctt ggtttcttcc
    18181 atttttcttt ctggggggcg cttcctttcg cgatcttcat gggggttatc ttcggcgtcc
    18241 tcctagggat ccccgttctc aagatgcgag gtgattacct ggccatcgcc accctgggtt
    18301 ttggagagat cattcgcatt cttgtccttt cggatttttt acggccctgg ctgggagggg
    18361 ctcagggagt ggggaaaatc cccaaggcct ctctgggagc gatcgaattt gccacccccc
    18421 agcagctcta ttatttgatt ctggcggggt gtctcctggt ggggtttatc tcctggcgcc
    18481 tgcgcgattc tcgcctgggg cgggcctgga tggcggtgcg ggaagacgaa gatgttgccc
    18541 aggccatggg gatcaacctg gtggccacca aattgatggc cttcgctacg ggagccggat
    18601 tttcggcttt gagcggcgcc atcttcgcca gcaaactcgg gtccgtctat ccccacagtt
    18661 tcaacgtcat gatctccatc aatatcctct gcgtcatcat tgtgggcggg atgggaagca
    18721 ttccgggcgt catcctggga tcgatcgccc tcgtgggctt gcccgaactc ctccgggagt
    18781 ttgccgagta ccggctcctc gtttacgggg ctgcgctggt ggccatgatg ctcctgcggc
    18841 cggaaggtct gtggcccgag gcagtccgac gccgcgagct gcacgaagag gaagcggaag
    18901 aagtcgagac acaacctccg ccggcttaaa agggtggatt cgggctgaat cctccattgc
    18961 gggctgaagg gtgagcagga aaaatttggc cgatccgaat ttcgaagaag gaaatccgga
    19021 agaagcagga aatttcaaaa tccgaatact caaaacaaaa aagtttggag ttttgatatc
    19081 tggattttga atttgtttcg gaattggctt gcttattgat gttgacaaga gtgaatgggg
    19141 atcaggaatg gcgattctcg cggcaaagaa agcgaccaaa cgattcggcg gccttttggc
    19201 catgcaggaa ctggacatcg aggtccagga aaagagcatt cacagcatca tcggccccaa
    19261 tggggcagga aagacgactt tttttaactg cgtgaccggt ttttacaagg cggaggaggg
    19321 ggagatcctt ttcaacggcc attccctggt gggcctcctg cccgaccaga tcgtccgcct
    19381 gggaatcacc cgcacctacc agaatatccg gctcttcccc gacctcaccg tgatggagaa
    19441 tgtactggtc gggctccacg ggcacttgaa agcgggattt ttcgggattg tggtggggac
    19501 tccgggaacc cggaaggaag aaaaggaggc ggctgaagca gcacggaaaa ttcttcgttt
    19561 cgtgaacttg gcggggaagg gagatttcct ggccaagaat cttccctacg gagaccagcg
    19621 gaggctggaa gtagcccggg ccttggccac ccgcccccag cttttgctcc tcgatgaacc
    19681 gactgccggc atgaacccca aagagacgca ggagatgatg gatttcatcc gccacctgcg
    19741 ggatgaactg ggaatcacta tcctgctcat cgagcaccag atgcgggtag taatgggtat
    19801 ctcggaaacc gtgaccgtct tggactacgg gaagaagatc gccgagggga gcccggcgga
    19861 aattcggagc aatgccgtcg ttatcgaagc ctacttgggc aaaggagggg gctcgcacta
    19921 ggggttttta tggccatcct ggagatcgaa catattcatt ccttctatgg tcatatccac
    19981 gccctgaaag atgtttcgct ggttgtagaa aagggggaga tcgtaaccct cgtcggggcc
    20041 aacggggcgg ggaagagcac gaccctcaag accatcagcg gtctgcttcg gccaaagcag
    20101 gggacggtcc gttttgaagg agaagagctc acccagtatc ctccccatga aattgtcggg
    20161 aagggggtcg ttcaggtccc cgaaggacgg cgaatttttg gccgccttac ggttttagag
    20221 aacctggaaa tgggggcttt cttgctctcg gaccgcaaga gggttcaggc caatctgaag
    20281 caggtttttt ccctcttccc caggctcgaa gaacgtctgg atcaggtggg gggaaccctc
    20341 agcggagggg aacagcagat gctggccacc gggcgggcgc tcatgtccag cccccgtctc
    20401 cttctcctcg atgaaccttc catgggactg gcgccagtcc tggtagacag catcttcgac
    20461 accatccgga agcttcatga gaccggcacc actatccttc tggtggagca gaacgcccgc
    20521 atggccctcc aggtagccga ccgtggatat gttcttcaga ccgggacgat cctgctcagc
    20581 gacagctcgg aaagcctgcg gcagaacgag accgtgcgcc aggcgtattt gggggaatcc
    20641 acagaataat ttcgaactgc ggatttcgga tgggggaata tttttacccg ggcgctgccg
    20701 tagtgaagta ctccgattct tctggggatt ccattgtgag tgctctgatc cggcggcact
    20761 gcaggaccgt atttcgggtt tacctattga accttagtct aatccgacca ggagaagccg
    20821 agacagtgat agagggaggg gcagctgaaa atctctccct cttttttttg tccgcggaaa
    20881 agggtatatt tctattggga accccgaggg tcgcggtaaa atgagaccaa atcagacccc
    20941 tcggaagctc aggaatcgtc aacgatgagc gtttatgcca tccccgcggc attagccttc
    21001 ggcatcaacc tgaccctctg cctgattgtc ttttatgaca atcccaaggg ggtggtgaac
    21061 cgggtcttca ccctcctcat cctttccttt gtagcctgga acgccggcga gctgatcatg
    21121 atcaccagcg acctccattc cgtcgccctg gtgggggtga aagtcatatt catcggcgtt
    21181 ttcctcttcc ccgtcttctt cctccatttt tcctacgtct ttccccaaaa aataaccccc
    21241 ctcttcgacg gctggcgcag cctcattctc tacgcggggc cggtggccct cctggttccc
    21301 tttatccttc ttcttcaggt ggacatcggg cgggtggtgc aaatcggctc catcttttat
    21361 tatgtcttca ctttccacgg gcgcttggag tcccagctta tggccctgat cctgggcctg
    21421 atcggcctcg ggtacttcac ctggggggtg gtcaacttca tcatttccta ccggcaaacc
    21481 aagatcgccc gacagcggct gcagatcctg taccttctgg taggaatcat ctccatgttc
    21541 gtcatcgggc ttgtcctcca cgtgatgaac tatttttttg acctgggcta ttccttcttt
    21601 tttgtggcca gcctgtattc tctcctgatc agcttcttct tcgccctggc catcgtgaaa
    21661 taccgtctgg tggatatcca cttcatcatc cggggcggga ttctgtattc gctcctctcc
    21721 ggtctggtcc tagtgatcta cgttctcttc atcaaaaacg tatgtgatat cctggcccgg
    21781 aagctggaga cgacttcgct cctggtggaa gctaccatgg tgctggtcct ggtcttcctc
    21841 ctgcgcccgt tggagaggaa agtggaagag ttcatcgacc ggttcttcta ccgggaaagg
    21901 tatttcttcc gcctgaaatt cttcgagttt acccgcaccc tcttgaatct tctggacctt
    21961 cagaccctgc tcgagaccac ggtcgccttt atctcccagg cccttctccc ggatcgggtg
    22021 ggaatctgga tcctggaaag cgagtcgaat tgctaccgac tcttcagcca gaaggggttt
    22081 tcccgggagc gttatttcgc cgcagacagc cctttcatcg cctacctgaa aaagtcggaa
    22141 agagcggtgg agctggagac ggtcaaggat tttcgagccc tccagcccga agtccgggaa
    22201 atgagtgatg cgcgcctttc cctggtcctc ccgctggaat gggaggaagg cctgctggga
    22261 ttcatcctgg tgggggagaa aaaggacggg aagaacttca ccatcatgga aattgaagcc
    22321 ctggaggctt tggctcctgc cgtggccatg gccatctccc gggcacgcct ataccaggac
    22381 ctgaaaaaaa aggactggca gatgatgcag tcggagaagc ttgcggccct gggggagatg
    22441 gccgcttcct tcgttcacgg gatccggaac cccctgggaa tcattctagg ttcggcagag
    22501 acccttaaga agaaggttcc ccagccggtc cagtcggaga tgatccagtt tatcagcgag
    22561 gagtccgacc ggatcaaccg catgctcacc aacttcctgg aattcgccaa gcccaaaccg
    22621 cccgtcttcc aggaggtgga cctgaaggag gtcctggacc ggaccctgga ttggatatcc
    22681 atcccggcgc ggaaacagcg ggtcgaggtc ctaagagaat acccagaaga aaaagtcctc
    22741 atccccgtgg accccgagca ggtccgcgag gctctggtga atctggagat gaacgccctg
    22801 gaagccatgc cccagggggg taagctgagg atcgtattga accagaagga taaccgggat
    22861 gtgactatcc gcctcagtga cacgggggga gggatctctc cggagctgga gtccaagatc
    22921 tttgatccct tcttcaccac caagcaaaag ggaacggggc tggggctctc catcgtccat
    22981 tcggtggtga agaaccacgg agggaccatc tccgtcatca gcaacaacgg agaggggacc
    23041 accttcacct tgaccctgcc tttccccaag gcgatcatgg ggtgcttcgg ggccgtgggg
    23101 ccggttcaaa acagcaaaga acagggttca gggcatattc ctgaatcgtc cgctcggagt
    23161 tcggagcgta aagggaggag caacgagtaa ccagtaacga gatgcgagcg acggaaaaag
    23221 gaaaacgaac gactggcatc agcgaaaaat ttgggggtct atgaaaccac gaatccttat
    23281 cgtagatgat gaagcccgaa tgcagaggct cttcgagatc aacctgggcc cgaagtatga
    23341 agtcatgacc gccggtgatg gggagcaggc gctggaggtc gttaaatccg gagatgtgac
    23401 ccttctggtc accgacctga agatgccggg gatgaacggg atgaccctgc tgcaggaagt
    23461 tcaccgggtc tatcccgatc tgccggtggt catcatgacc gcttatggca cggtggaagg
    23521 ggcggtccag gccatgaagg aaggggcggt ggattacatc ctcaagccgg tcaagatgga
    23581 ggaaatggag ttcctcattg agaagaccct ttccgtgcgc cgccttcagg acgaaaacag
    23641 gtctctccgc caggaactgc agagtgttta cggtccagcc agtattgtcg ggaatcaccc
    23701 ggccatgcag aaaatcatcc agctcatttc tcaggtggcc gggaccaaag ccacggtgct
    23761 ggtccagggg gagagcggga cgggaaagga gatcatcgcc cgggcaattc attaccagag
    23821 cgaccgggcg agcaagccct tcgtggtcat caactgcgcc gccatcccca gcaacctcct
    23881 ggagagcgag ctcttcggcc acgaaaaggg ggcctttacc ggggcgatca agaccaaaaa
    23941 gggccggctg gagctggccg atcagggaac tctatttttg gatgaaatcg gcgaaatgcc
    24001 caaggaattg caggtgaaaa tccttcgagt gctggaggag cagaaattcc agagagtggg
    24061 gggcaccgaa gatgtggagg tggacaaccg gatcatcgct gccactaaca aggacttgaa
    24121 gcaggccgta gaagccgggg ccttccggga tgacctcttc tatcgcctga atgtgatcac
    24181 catctccatc ccgcccctcc gggaaaggaa agaggatatt cctctgctcg tcgattattt
    24241 cctggagaaa caccgaaaga tattcaaaag ccgggcaacg ggggtgagcg atgaagccct
    24301 taaattactg gcggagtacc cttggccggg gaatgtgcgg gagctggaga ataccctcgt
    24361 ccgggccatg atcctttgcg ggtctgagtt gattcagcct ggcgatttac cggaggggct
    24421 gggtccggaa gaaattgaag aagaagtgag gccgcccacg gaccgggaag aactgaagca
    24481 gatgaaaaag gtggcccagc agaaggcaaa gggggagatt gaaaagaagt ttatcattga
    24541 agcactcaag cagggggggg gaaacgtcat gcgctcggca gaaatggtgg gcatggaccg
    24601 gagacaattc cagaaccttc tccgaaaata cggcatttcc cggaaggact ttcttaaaga
    24661 ttagaggaga aagagagtgt aaagtgcgaa aaaacgagtg taaagtgcga aatggtcatt
    24721 cgcactgtta agtatttaac attaatcatt attccgactt ttcccttcca cttgcattaa
    24781 attctcgcat cttcccagtt ggttagcggc cccgtctata aaaaaatcta ataatattaa
    24841 attattagga aaatatttcg aattttttgg atggctcaga ttttgcctgt attcccctta
    24901 aacggaataa atagggagga tacttatggc catcggagga ttaattcgcg gcctgcgcaa
    24961 gaaaactgct ttcaaagact ggcgaaagat tgagccttat taccccccga cccaaaatgt
    25021 tcccctaggg ctacccaagg aagtggaaag cctgtgcccg gaatgcggga aaatcattcg
    25081 cgcctccatt ctggagcggg gcgggaaagt cttcatggag aagacctgtc cggaccacgg
    25141 tttcctccgg gacctggtct attcggatgc cgagctttac aagaaatgcg agcgctggac
    25201 ttatgaagac gctgacggaa ttctgaaccc ccaggtcacc ggggccaaag tctgccccga
    25261 tgactgcggc ctgtgtgatc tgcacatcag ccccccgatt ctggcgaaca tagacttgac
    25321 caaccgctgt aaccttcgct gtcccatctg cttcgccaac gccaacgtgc aggggtacgt
    25381 gtacgagccc tcctacgagc aggtggtgga aatgctcacc ctgatgcgta acgtaaagcc
    25441 ctttttcccg ccggccatcc aattctccgg gggagagccg accattcacc cccgcttcct
    25501 ggatatcctt aaggccggtc ggtccatggg atttagccac ttgcagatcg cgaccaacgg
    25561 gattcggatc gccaaggacc cggaatttgc caagaaggcc agagaatccg gactctatac
    25621 catgtacctg cagttcgacg gcacatcgga cgatgtgtat aagaaaaccc gcggcctctc
    25681 cggtctctgg gaattaaagc agagggcggt ggagaacgcg agggccgcgg agctccgcgt
    25741 ggtcctggtg ccgaccatca tcaagaccat caacgacgac caggtgggga acatccttca
    25801 gttcgcggtg gacaactccg atgtgatcag cggcatcagc taccagcccg tggctttcac
    25861 cgggcgaatt tccacggagg agagggagcg gcagcgctac accctctcgg acctggccat
    25921 agacatcgag gcccagacgg accttctccg agcgcgggag gactggtatc ctttgtcggt
    25981 gacttcccca ttctccaagc tcctgagctc catctggaag tacgatgtgg ccaagattac
    26041 ctcccactcg gactgcggga tcggctccta catcttcgta aaccggaaaa cgaggagtgt
    26101 gacccccata acccaggcca tcgacatcga ggggttcact tcggaactga acaagctggt
    26161 gaaacagaag atctccaaca tccgggccct caccgccctc agcgctcagc acttgctgag
    26221 aaagttcttc cgggaagacc gggccaagga cggtttgacc gcggagatct gcctggagct
    26281 ctttcagggg gtcgtagaca aggaagccca gaggagagtg gagctgatct acaactggga
    26341 catgctgctc gtgggcggca tgcacttcca ggattcctac aattacaacg tggcccgggt
    26401 gaaacgctgc tgcatccatt acgccgcccc taacggtcgg atttatccct tttgcaccta
    26461 caattccggc ccagtcttcc gggaaaagat cgaacgccag ttctccgttc ccctggagga
    26521 gtggcgcaag cggaactggg cccaggaaga tgcggattat tacctctgcc ggacatgaaa
    26581 cctgactgga gcggaaaaag ggaggcttaa agcacctccc tttttttatg ctcagaacag
    26641 aattggaatt cagcatgcag ggggcgggga tttggtcaga gaaagaaacg aacaatgttc
    26701 cttctccgca cccagcactc aggactcagc ccttttgggg ggttcttccg gattcgtccc
    26761 ctgttcttcc tgcttggatg gcggaggctg tgcggggggc ttgggggaag gggtttggta
    26821 gtaacggtag aggagaagaa cgccgataat caccaggatg gtaggccaga tcaggtcgct
    26881 ccaggcctcc acttccggga agaaggcgat gacgaagttg acgatgaaga ggatgacccc
    26941 gattccaccg acaatccagc tggcaagact ctgggggttg atgaggaaaa gggcgatccc
    27001 gacggccacc aggataatag gccagctgat gtaaatctcc agaatgatca gccggtcaag
    27061 gataaataga gcccccaccg ttaacagaac caggccggca atgagctgtg ccttctttcc
    27121 ccgcatgatc tcttctcttt ttcgctccgg tatatttgaa atttagtaac acgtctggta
    27181 tggtaaatca agaagaagct ttctgcgttc ggccttcagc aatcagccaa tgatcttttt
    27241 gtggctgaag ctgcatgctg accgctgatg gctgagtgct atgattatga tttcatttat
    27301 tgagaaaata aagcgccatc gattctacgc cccccaggtc gtctaccacc aaccctttcc
    27361 ttctgagaga gccaattttt cccgcccggg aaaagacttt tccccgggcc tggcccgggc
    27421 tctggagaaa atgggaattg gccgccttta ttcccaccag gtggaagcca tcgagcaggt
    27481 tggccggggg aagaacgtgg tcatcgctac tcccacggcc agcggaaaga ctctgatcta
    27541 caatctcccc gttgtggaac gactgtttca gaattcggaa gacaaagctc tgtatctctt
    27601 tcctctgaag gccctggaac aggatcagct gaaggctttt caggaatttg tccaaccttt
    27661 ccaggcccct gttccgttta aggcggagat ttatgacgga gacacttcca gctaccgccg
    27721 caagaagatc cgggagtcca ttcccccagt gctcatttcc aacccggaca tggtccacct
    27781 cagcctgctt ccgtttcatg cccagtggga aactttcttt cgcaacctgc gctttgtggt
    27841 gatcgacgag ctgcacactt acaagggtat tttcggctcc catctcaccc aggttctccg
    27901 acggttggaa agggtctgcg acttctacgg ttccaccccc cagatcatcg cctgctcggc
    27961 gacgattgcc aatccccagg cctttgccga aaagctgacc ggcctccctt ttgaagcggt
    28021 gacccagagc ggcgctcccc ggtcggggcg acacttcctc ttcctgaatc caaataccag
    28081 cccctatacc ctggcggcca agctctttct ggactgtgtc gacggtggtt ttcggactct
    28141 ggcgtttacc caggcccgaa agatcaccga actgcttcac acctgggtgc tgaaggaccg
    28201 gccggacctg cggggaaagg tcagctctta ccgggcggga ttcctcccgg aagagagaag
    28261 ggagatcgaa accaagctgg tctccgggga gcttcacggg gtgatctcca ccagcgccct
    28321 ggaactgggg atcgatatcg gcgggctgga tgtgtgtatc ctggtcggct acccgggaac
    28381 ggtggccgcc acctggcaga ggggaggacg ggtagggcgc gccgaccggg aatccctcat
    28441 cgccctgatc gcccagcccg atgcgctgga ccagtacttc atgcaccacc cccaggattt
    28501 cttcgggaga gggttcgaaa gcgccgtggt ggacccggac aattccgtgg tggtctctaa
    28561 acacctagtc tgcgcaagcg cggagatccc tctgcgaacc gacgaatcga tcatcccggt
    28621 caagaattac gccccccttc tggaccagct tgtctccgac ggagagcttt tgaagagcgc
    28681 gtccggccgg gaatggttct cccatcgcct gaatccccac cgcctggtgg acatccgcag
    28741 cgccggagaa gggttcacca ttttcgaaga agaaagtaag cgtttgatcg gccagatcag
    28801 cgtgccccgg gtttacagcg agtgtcatcc gggagcgatc tacctccacc gggctgaacc
    28861 ctacttggtt acccatttgg atcagggcaa acgggaggta tgggccaagc aggtggaagt
    28921 ggactattac acccgggccc tctcggagaa ggagacggaa atcctttcaa ccgtccgcac
    28981 ccaggctttt gcccacttca ccgcctgcta cggaaggctg aaagtgacgg aacgggtccg
    29041 ggggtttgaa aaacggcgca ttttcggcca ggatttgctc agcgtacacg aactggagat
    29101 gccttcccat atcttcgaga ccatggggct ctggatcatc ctgcccggag agatgcccca
    29161 gcgatttaag gaccaagggc tccacttcat gggcgggatt cacgccgtcg agcacgcctc
    29221 cattgctctc ttcccccttt tcgccctctg cgatcgaaat gacgtggggg gaatctgcta
    29281 tcccaatcat ccccagctgg agaaaccggc catcttcatc tatgacggat acccaggagg
    29341 agtgggactg gctgattacg gatacggggt catcgaagaa cttttgagga aaagcctggg
    29401 gatgatccgg gattgtccct gcgtggatgg ttgtccttcc tgcgtccatt ctcccaaatg
    29461 cggttcgggg aacaagcccc tggacaaaag ggcggcagag tttgtcctgg aatggcttct
    29521 gggagaatcg aagatcgaaa agaagggaag ggggaagggc agggttgcgg tcaaggatga
    29581 ggtcttcccc acgccctcgg atatctttgc cggagattca gaattaagag aaccggaatc
    29641 cgtggcctgc gcccctgctc ctgcctggct gaaagaagaa ctggcccgac atcggattct
    29701 tttcctggac atcgagaccc agcggggtgc ggaagaagtg gggggctggc acaacaaaca
    29761 cctgatgaga gtgggggcag cggtggtcta tgattccctg gaagatgttt tcctgatatt
    29821 cgaagagaac tgcgttcacg agctgatcga aaggttgaag accgcggagt tgatcgtcgg
    29881 cttcaacatc gtcgacttcg actaccaggt tttaaaagcc tatacctcct tgcgtttcca
    29941 gcagctaaag acctttgaca tcctgcagga ggtttcccgc cagctggggt accgcctttc
    30001 ccttggtcat ctggcccaca agacgctcaa cacggagaag tccgccgacg gcctccagtc
    30061 cctccagtgg ttcaaagaag gcaggatcga agaagtcatc gcctactgca aaaaagacgt
    30121 ggagatcacc aaagacctgt tccttttcgg cctggccaat gggtatctcc tgttcgagac
    30181 ccgggcgggg caacgggtaa gattgccggt ggagtggaag ctggagcgaa tactcaagga
    30241 ataaataggg ggaaaagggg aaagggcgaa atggctaaaa aatcctcaat cccagtcctt
    30301 ttcgcctctt ccccgtttcc ccttttcgcc ctttctttca gcagacccga cacagcagaa
    30361 taggtacggt cgccgtgtgg atgatcttgt tggccacact tcccaggacg aactggctga
    30421 ggccggtgcg gccgtgggta gacatggcga tgaggtcaaa gtcacggtcc cgggcatgat
    30481 cgacgatttc ctgggcatca ttgccgtacc ggacaacgga tttcactctc acgcctacgc
    30541 tcctcaggtt gttttccact ttcgccaggt attcctccgc ttcccgaacc acattcacct
    30601 gatgctggac cgggtccatt cccggaaaag tgtgggcgag agccacgcga atcagggtca
    30661 cttcaccgtc gtggaccttg gccaaccatt caacccgggg aacgatcccc tcggccaatt
    30721 tagaaccatc cagtggaacc agaatctttt tgaacatgga actggcctcc tttcttaaat
    30781 gccgaataag aattcaatgc ttttcattgg catggtacgc attatatcca aagggcactc
    30841 caaaatccac actcccccac cgcagaccga ttttttggaa aggcaaagaa cttcaattcc
    30901 gcattccaaa atccgcattc aaatcagtca ttctttcgaa ggactttctg ggagaagacg
    30961 gcaaaaccca ccagcgccag accgatcaca tcgtaaaccc aggagggata aaccagggct
    31021 agaccggcga tgatcagaat gatcttttca taccaggtcg ccttcttgaa catccaaccg
    31081 tccattccgg ccgccagcgc aaagatcccc accagggcgg ttacgaaagt ccaaataatg
    31141 ccggcggggc ttcccttgag cagcaaggcc actccgtcgg gatggagggt gaaattgaag
    31201 ggtacgagaa aagcgggcag ggtgtatttc caggcttgca tggtagtctt ataggggtca
    31261 ccgcctgtca gggcagcggc ggcaaaagga gagagagccg tggggggcga aacctccgac
    31321 aggatggaat agtagaagat gaacatgtgg gcggcaaagt cgggaacgcc cagcttgatc
    31381 agcgccggag cagcgataac tgcacaaata atataagagg ccgtcaccgg cacggcaagt
    31441 ccaacgaccc atacgacaaa agccgtaaaa atggcggtca aaaggaggtt tccccccgcg
    31501 tattcgatca cgatagagct gaatttcagt cccaagccgg tgagagtcgt caccccaacg
    31561 ataatgcccg cagaagcgca ggtagtggcg acggaaagca ccccaataga ccctcctcgc
    31621 agagcagcga tcaatttcct gggaaccaac gccgtgtccc gacggatgaa gcttaccgcg
    31681 aaggcgatga ccgtagacca aaagacggcc aggatgggag tgaaccccag aaccataatg
    31741 accacgatgg agatcagaga ggtgaaatga aacccataga gtcgagtgag ctgccccaga
    31801 gaataggtgt gatcgaaggc aacactcttg gcgccaaact tcttggcatc aaattccacc
    31861 atgaggaaga tggcccaata ataaaggatg gtgggaatgc tggccatgac gatgacgtct
    31921 aaataggaga tcttcaggaa ttcagcgatc aagaaagcag cggctccaag gacgggaggg
    31981 gagagaatcg cgccgatgcc ccctgcggaa aggagaccgc cggcagcttc cttgttgtat
    32041 ccggccttgg ccagcatggg ccaagccacc gcacccagag tcaccgtcgt agccacaccg
    32101 ctcccggagg gtcccccgag aaggaaggag gccagggtca cggtccgccc cgcggtggtg
    32161 ggctttccgc ccatggccgt gagagaaaag tcgataaaaa acttgccggc gccggaaaac
    32221 tccaaaaagg ctccgtagat agtgaagaga atgatgaaag tcgaggagac gtcaatgggc
    32281 acgccaaaga tcccttccag ggtcatgtac atgtggccca cgagccggtc cagcccgtac
    32341 ccccggtggg tccagggggg cgggagccag ggtccgacgt aggcataaat gaggaaagaa
    32401 cccgcgacca gaggcaaaat ccagttggtc gtcctccggt tggcttctag caccaaggcg
    32461 atcagcaaag agccgaaaaa caggtccccg taagtgggag taaccgagcg atagatgaac
    32521 tcctcaaaat ccaggaaggt gtaaactatg caggcgatcg ccaggatgga gagaaggtaa
    32581 tcataccaga aaatctggtt gcgataccgt ttcaaggaag gaaagaccaa gaaggtgaga
    32641 aagagaacga acatgacgtg gatcccacgg aggatttggg tcatgatgat tcccacggca
    32701 gcgtacagat ggatgaggga catgatgacc gccaccacag tgatgaaaac gcccattttc
    32761 cccaccagtc ggcgagacgg cccctcttcc tcctcgatgt aggcttcggc tttcttcaag
    32821 cgctcttcgg taatgaaatc ttcttccgcc accggggccg ttttcttttc ttcggccatc
    32881 aacttttctc ctttaccacc cgggacagga gatatttccc ccaggtcatt cgcaccaccc
    32941 ggacttccag tcggtctcct ttttccccta ggtcccccaa ggaaatcttc ttctcgccgt
    33001 gggagagcgt ctgagcctga gacatgccca gacggagagc aaaggatttc atcttgcggt
    33061 ccaccggata gtaatcccgg ttgtcctcct caaacccgta ataatgggct acggaggggc
    33121 ttcgggtcct caccccccga aggaggatca cctccccttc ttccacctcg aactcttcgg
    33181 cggccgcttg aagataaatc gagtggatat aggagatgga aaaacgggtt ggttcaccga
    33241 gccgcaggtt cagtctggcc ggtcgttccg cattcaccac ttgaagaacc ggcacctcga
    33301 aaatgaacaa ggctcccccc gcagccgcga gggtcagaag agccaagagg gggagccatc
    33361 ctttgatttt caactaggga accttaattc ctttttctgc gtagtactta agcgcccccg
    33421 gatggaaggg aatggaggaa ccggttaccg caccctggag ggataaatgt tcggcagtct
    33481 tatgaaccgc taccaaatcc gatttatgct ccagaatcgc cttgacaatt gcgtaagcca
    33541 gaccgggatc gaaggattcc atgcaaacca gcatggtggc cactccaatc acggggacgt
    33601 ccatttccat tcccgcgtag gttcctttgg ggatgacggc gttgtaataa accggaccat
    33661 atttgcggtt gagcgtcggg agagccttgt cttggggaag aattcgggcg gaaattcctg
    33721 gagtggccgc caggtccata accgccgccg tggggagacc tccatcccag cagaaagcat
    33781 cgatcttgcg gtccttcaag gctccggccg attcggcaac gctgagccgt tcccggcgga
    33841 tttcttttgc cgggtcgact ccaaaggctt ccagaatccg ggaggatttg acttcggtgg
    33901 cgcttcctgg agcccccacg gagaccttct tccccttcaa ctccgggacc gacttgatgc
    33961 cgctgtcctt gaggactacc atgtggttca ggtttccata caggaccgcc agggtgcgca
    34021 aggggatctt gccatctttt tcgaaccttt cttttcccag gatggcatcg ttggccacat
    34081 cggcaatcac cagggccaac tcccccttgc cggccttgag aagtttgata ttgtccacgg
    34141 ctgccgcagt gacttccgcg gtggaggaag tgttggggac atacttggat ataacttgag
    34201 ccagcgcgcc acccaacggg tagtagaccc ctccggtccc accggttagg agggagagcc
    34261 ggttcatctc cttggattgc gcccaaccgt tttcggagga aaaaaggaaa acgaagagaa
    34321 taaggaaagc aagcccatat ttacccttac ccatcatctc tgtcccctcc tatattggtt
    34381 agatgaaaaa tgagacggct ttttttctaa gagatggttg aatcagacac ggcctgccgc
    34441 taaaggtgaa cccccccaaa ccattgaatc cccttccctg gatttcaact cgcccctccc
    34501 cctgaaaaat ctttcagagg aaggtgcggg agccagaccg ggggcttgca attcctttat
    34561 ccaggactta ttggaccttg attccttttt cccgatagta tttgatcgcc ccggggtgga
    34621 agggaaggga ggaacccacc gttgccgaag ccagagtgaa attgctggcc tctttatgga
    34681 cggctaccaa ctcgggctga tgctcgagga ggaccttaag aatgttgtac accaggctct
    34741 cctccatctt ctcccggcag atgaggagat tggccacgca ggccaccggg acatccgcat
    34801 ccatgcctgt ataggtgttt cggggaatgt cacgcttgaa gtaaatgggg ccatatttct
    34861 ggatcatccg gtcgaggtgt tccgcattgt tcaagatgcg gattttgact cccggggtcg
    34921 cagccaggtc catgaccgcg gccgtgggca ggcctcccac ccagccgaag gcgtccactt
    34981 tccggtcttt gagggcgccc gccgattcag ccacccccag gcgttctttt ttgatgtcct
    35041 tatccggatt gattccgtag ctctccagga ctctcagggc catgacctcg gtcccgctcc
    35101 cgggggaccc ggtggaaacc cgctttccct tcaagtcggt gatggatttg attcccaggc
    35161 cgtcgacggt cacgaagtgc atgtaattgg ggtagaccac ggcgaccgtt cgaatagcct
    35221 gggggcccgt ggttttgaac cgctccaaac ccttgtcggc gtcatagccg gtatcggcca
    35281 tgatcagggc aaggtcggct ttcccggcgt ggatcagctt gcagttgtct acggaagccg
    35341 aggtcacttc ggcggtggcc tctgtgttgg ggacgtactt ggagatgaca ttggccatcc
    35401 cgccccccag gggataatac acgcccccgg tgcctccggt ggcgagggag agccggttgg
    35461 cttttgtttg cgcccctgcc gcgccggaaa aaacgatgaa gccggtgaag atgaaaatca
    35521 gtgaaaagac ccctccggtt ttggttgcca tgtcttcctc cttttcatgg gcaaagggca
    35581 tagagttaac ggatttaaga aaatcaaaag actcttgcca ctccgctcat ttcccaacgc
    35641 aaaaaaagaa gggcatcgaa caacaagccc agggtggaga ggataagacc caaggcgcta
    35701 tgctctattc cctccactcc ctgaggcttt aaattacttt gccttccttc agacgggcga
    35761 ttttttcttt atcgtatccc agcacttcgt gaagaaccga ttcggtatgt tctcccagaa
    35821 ggggaggggg aattcggtat tggacgggag tcttggatag tttcatggga gaccccacga
    35881 gtttgaactt gccgaccttc gggtgcccca tctccaccag catctcccgg gccaagacct
    35941 ggggatcggc aaagacccgg tcgatggtgt tgatgggacc gatggggatc tcggcttcac
    36001 cgaggagctt gagccactcc ccggagtctc gttggaggaa aacttcctgg agaaggggaa
    36061 cgagtttctt gcgattttcc actcgtttcg ggttggtggc gtactggggg tcgtccatga
    36121 gatgctcgat ccgggcaatc ttgcagaact tcttccactg attgtcgttt ccgaccccca
    36181 gggcgaggta gatgccgtcc ttggctttga aggtttcgta gggaacgatg ttggggtggg
    36241 cgttgcccat ccggcgggga accttccccg acaccaggaa gttgctggcc tggttggcca
    36301 gccaggctac ctgggcgtcc agaagggcca tatcgatgta ttgcccttcg ccggtctttt
    36361 cccggtagtg aagagcagcc aggattgcgg agcaggcaaa aaggccggca gtgatgtcca
    36421 cgatggccac ccccaccttc atgggagaac cttcttcctc accgatgatg gacataagcc
    36481 cgccctgagc ctggaggatg aagtcgaaac cgggaaggtc gcggtagggg ccgttttgcc
    36541 cgtacccggt gacggaggcg tagataattt tggggttgat ggcccggaca tcttcatagc
    36601 ccaggttcat tttggccagg tcaccgggga ggaaattctc cagaagaatg tcgctccgct
    36661 gggccagacg gcggatgatc tcctgtccct ccttcttctt gaggtccacg gtgatgcact
    36721 tcttgttccg gttggtgcac aggtaataag cgctcagctc ccctacccaa gggggacccc
    36781 agtgacgggt ctcatccccg accccggggc gttccacgcg aatgatttcc gcccccagat
    36841 cgcccaacat catactgcag taaggcatag ccaggacccg cgaaaggtcc aggattttca
    36901 aatcagccag ggcttgcggc atagaattcc cccagctgta ggggcaattc atgaattgcc
    36961 cctccgctca gttgattaga ggttgatgtt ggaatatttc ctccagggcc gtgcttcgga
    37021 cttattctcc agcacgtcca ggcagcggtt gatgatcttt cgcgtgtcgc ggggcatgat
    37081 gacgtcatcc aggtaattcc gttccgcgcc ccggtacggg ttctcataga gatcgctgta
    37141 gagctggatg cgctgcctcc gggtctcctc cgggttgggg gagcttttga tctctttggc
    37201 gaagatgacg ctggccgcgg tgtcggcgcc cacgatggta actttcgcct ggggccaggc
    37261 gaacatgaaa tccgccccgg tgtccttgca gcacatcccc aggtaggccc ccgcatagga
    37321 tttgcgcaca atcacggaaa tcttgggcac cgtagcgtcg atgtaggcgt aaagcatctt
    37381 ggctccgtga cggaggatgc ccttccactc ctgttccggg ccgatccagt acccgggggt
    37441 atcgtggagg ttcaccaggg ggatgccgaa gagatcgcag aagcggacga aacgggccac
    37501 cttgtccgcg gcgtcgcagt cgatggcccc cgccttggac atgggctggg aggctacgat
    37561 ccccgcgggt cggccgtgaa aacgggcgaa accgacgatc acattagtgg cgtgattttt
    37621 ctgggtctcg aagaaggacc cgtcgtccac gatgcgggca atcaccttat gcatgtcgaa
    37681 aggcttggaa ggggtctcgg gaatgagcgt gtccagttcg ggagtcgggc ggtccgggtc
    37741 gtccgaagtc gctttccggg gaggcctctc ccggttattc gacggcagaa aggccaggag
    37801 ctctttggcc cggtccaggc agtccttgtc gtcttcggcc acgtaatgag tttggccggt
    37861 cttataggcg tgagccttga cgccgcagag ttcttccagg gagatttcca cgccggtttg
    37921 agtcttaacg aaagccgggc cggcgatccc catgaaaccg gtcttgcggg tctggaagat
    37981 gaagtcctgc atgatgggat ggtaggcctg cccccccagg cagggtccca tgaggagggc
    38041 aatctggggg acgatcccgg atgccaggat ctgggagcgg aaaagccagg cgtaagcctg
    38101 gagggtatcc atcccctcct gcaggcgggc gccgcctgaa tcattcatgc ccacgaaggg
    38161 ccagcctttg tccttggcga agtcaatggc ctgggagaat ttttttccgt ggtattcgcc
    38221 gaaggtaccg gccatggccg tatagtcctc ggctcccacc accaccattc ttccattcac
    38281 cttgccgaat ccggtcacca cgccctcggc gggcacctcg cgcttgtcca taccgaaggc
    38341 ggtggtgcgg ttacggacga acatccccaa ctcggtgaag gtcccggggt caaaaaagta
    38401 ttcaacccgc tcccgggcgc tcattttgcc ttccttatgc tggccggcga tggcttgcgg
    38461 gcctcccatc tgtttgattt tggtggcccg ctcctggtag tctttgtaca gggcttcaaa
    38521 gcccttttct ttgtattcca tggtaaggcc ttctcctggt taccggttgg ctttgcggta
    38581 tcccaactgg tccatggcga taatcgtctt ttggatgttg gccgtgcctt cgacgatgga
    38641 aatggacttc gcatctcggt agtaacgagc caccgggtat tccgtagaat agccgtaggc
    38701 gctgagaatc ctcatggcga agtccgccgc ccgtccagcc acctctccgg catagtattt
    38761 ggccatggag gtctccagca cgttattcag catcccttcg tctttctgca atgccgcccg
    38821 gtacaccagg aagcgggccg cttcgatctc cgtggccatg atggcgatca tgtcctgatt
    38881 catctggtac tggccgatct gctggccgaa ctggctgcgc tcgttgcagt acttgaccgc
    38941 tgcctcgagg caggcctggg cgattccgac acctccggcg gcgcaggaga ggcgggtctg
    39001 gttcagggac ccgaaaacgt gtttgacccc ttcgcccagc tcccccagaa cggcttcctt
    39061 gggaaccttg acatcttcca ggatgatctc tcccgtgggg gcagaatgga gtcccagctt
    39121 gtccaagggc cgggtggaga ctccaggttg gtgcatatcc agaaggaacg tggaaagccc
    39181 tctgctcttg gccgccgggt cgtggtaggc atagagaatg cacaggtcgg ccacctgggc
    39241 attggagatc caggtcttgg agccgttgac caggaagaaa tcccccttgg ggatggcggt
    39301 ggtcttcatg gacatcacgt cggagccggc gttgggctcg gtgatggcaa agcagctgat
    39361 gaattccgtg gaaaccagct tggggatgta tttctttttc tgctcttcgg ttccgtagcg
    39421 gtagatggag agggcggggc cgaaagtctg catgttgaag gtgacccgga gagaggcgct
    39481 ggccctggca atctcctcgg tgatgattgt cccggccagg aatcccatcc cattgccgcc
    39541 gtatttttcg gggaccaggg ttccgtagaa gcccaagtct cccattttcc tgatcacttc
    39601 ccgggggaag tagttttcat tctcccattt atccgcaaaa ggaatgattt ccttctcggc
    39661 aaaatcacgg gctgtttcac ggatggcttg cagttcctcg cttagcttga agtccatatt
    39721 tccctcccgg aaaaaagtca caggtctcaa gtctcaggtc ccaagcaata gttcccaggc
    39781 ttcagacccc aggtgtcgag ttcgagaatt acggcttatg acttgtggcc tgagacttgt
    39841 gacttgaagc ttatgacttt attcgatgac tgccaggacc atgtcggttt cgcaggtctg
    39901 gccgacggaa accttgattt cctttaccac gccggaggcg ggagccacta tgggcatctc
    39961 cattttcatg gcttccagga cggcgatttc atcgtcttct ttgacctggt ctccgacttt
    40021 cactttaatg tccacgatct ttcccaccat gggagcagtt acgttatggg ccatacagag
    40081 tctcctttcc gtttcctttt ctttttgatt taaaaccgca ggaagcgcaa cccaaaggtc
    40141 tgcttgacgg tttttccccg aggaatcttc agggaggaac gtcttcaccg aactatccac
    40201 ctataaccta tggtttttat tttgtcaata tgaatagttc cttaaagttt tgggcacctg
    40261 aagggcttcc atcggccaaa ttcggaaggg gaaaaagggg tgggggtggg cccatcggaa
    40321 atctccttgc acccttccat atttttgaag ttgacttttc ctcatggggg tatcaatatt
    40381 tacggggtgt agaaaaaccg tccgggttgc cacgggttcc acctctgaat ggaaggaatg
    40441 attttcctcg atgatgcgct cccaaggatt ccagaaattt gccaggaggc tgaacctctg
    40501 ggtggcttat tttgtccccc actcctttct cttcgttatc ctgctaacct ttctcgtata
    40561 ttttttgggt atcctggtta cctaccaagg ccccttcgac atggtacaat tttgggtccg
    40621 gggagtttgg aaccttcttt ctttttccat gcaaatggtt ctcctggtgg ccgccgggtt
    40681 cagcgttgcc agcgccccct tgagccaaag agtccttcgt tttctggccg cccttccctc
    40741 ttctccccgg ggggcggtct tgtttctcac cgcctttacc gccctttgct ccctgttgca
    40801 ttgggggctg ggaatcatcg ccggctcttt tttggccaga gaaatgggga gaagagtctc
    40861 cggcatcgat tatcctcttc tggtggccag cgcctttatc ggaatggcct ctggaaactt
    40921 gggcctcttc gcctctgacc ccgtagtgat tcgaaaggcg gccccttttc tagaaaaagc
    40981 catgggctca agccccaccc cggaaaccgc ctggagtcta gtgagccttc tagggttcat
    41041 ccttggattg gccgctgtta tggccatgct gggggtcctc tcccccgaag agaaggaggc
    41101 cactcctccc gggcgagaac tgctggagcg ctttgaaaga gaggaccggg ctgagctagg
    41161 taccatcgcc gcagagcggg aggtgcatca gaaagggaaa ttggcctttg gcgtatggct
    41221 tgaacacagc cgctggccgg tatggcttat ctccatcatg ggcttttcct acatcgtttt
    41281 ttggttttac gggcaaggct ttgccttcaa cctgaatact ctgatctttg tccttttcac
    41341 cctggccctt tcccttcatg acacccccat gcatttcctg caatctctgg aaaactcggc
    41401 ccgggccgcc cacgggatca tcgtccagtt cccgttttat gccggcaccc agggcatgct
    41461 ggcctcctcc ggacttgcgg ccctggccac ggcttgggtt ctctggctga tcggcccggc
    41521 tacctacccc accctgattt ttctccatgc ggtggtgctg aacctcttcg cccccacatc
    41581 cgggggcgtc tgggaggtcc aggggcccct gatggcaaag gccgcccaga gcctggagct
    41641 tcactttccc cgggtcatcc aggccttttc ctcgggggaa atgttgggca acgtcattca
    41701 ccccttctgg acgattcccc tgatgggaat ctgcggcctg tcggtgaggg atatcatggg
    41761 ttactgtctg atcgccttcg tccttctttc agccatatgg gtcttgtgcg tcaacctttt
    41821 gccagtttag ttcaataagc aaagctggca gctgataggg cttggaaaag cccatgaatg
    41881 atcagtcgtg agctatgact cttggagggg aggtttaatg cctgaaatca ccagccgtga
    41941 ccgaatccga gcggcgatga agcggacgta tgccgaccgc attccctaca gcttgaccct
    42001 cgggcctttc cgggcgagag ttctgggatg ctccccgcgt caatattatc aagaggggag
    42061 taaactggcc cattctactt tgatctgtca tgaaatgttc cagcatgaca gcgtggaggt
    42121 gggctgggac atcttcctgg aggctgaagc agcaggggcg gaattggaat ttccggacga
    42181 tggggttccg caagtgagaa aacacgcgct ctctcggaag gcggcgattt cggctctcca
    42241 aattccccgg cctgaatcca gcgggagata ccccctctat ctggaagcct gcaggaccgt
    42301 ggcaagttcc cttcgggagg tggccatgtc tggaacggtt actggcccgt ggactattgc
    42361 tgccggtctg agggggactc aggagttgat ctacgacacc gtggacgatc ctgggtttgt
    42421 gggggagctc ctaaaattca ccacggaagt gacccagatt ctgggggccg gggtggtcga
    42481 gaccggtctc ggcctgacca tgggggaagc ggcttcttcc tgcagcctga tctctccggc
    42541 gatttaccgc cgcttcatca agccctgcca ccagaatatc gtccagttct ttcgggagaa
    42601 aaaaataaac ctttccctgc atatttgcgg acacatcgat cccctcatgg aggacctgat
    42661 ggatttgggg atcgtggcct taagcatgga cagcccgtct tccctgaaaa ggatggtgga
    42721 ggtctcccaa aagagaatcc tccttttggg aaacgtggcg acttctcttt ttgtcagcgg
    42781 aagccgggag gagattgaga actcggtgaa ggagtgtatt cggaccgcag cccccggcgg
    42841 ggcgttcatc ctgtcctcgg gctgcgagct tccctacaat gccacggtcg agagggctcg
    42901 gttctttatc gccgcggcca ggaaatatgg gaggcccgag tggtttctcg ccaaatagtc
    42961 gcatggtcga tcggtccggg agttgaatgg aaccattgtc gttggaggat ttttccataa
    43021 ggcaggacag gctaaaattc aaaaacccat cggcatcatg acgacccgac tatgtgactc
    43081 aatgacgacc tgaactgaag cccgatgcct atccctcaaa ccattcttca actcccaagt
    43141 ttttccttga caatcaaaat acccattcta taatttcact tattttttct ctcctaatta
    43201 ttcagcgaag gacgaaacat gtacacttgg ttttatggtt tttccgagga gccttttaac
    43261 gttaaccctg accctgtctt cctctatttg acgaaaaccc atcagcaggc tttggacgat
    43321 atggtccggg cccttcagga aagaaaggga tttgccttga tcctgggcga tttgggcagc
    43381 ggaaagacca ccctgatcca gtatttcctg aacacggtcg atccgaaaac cttggtcaag
    43441 gctatctttc atccccccgg aacgatcgaa gacctgctgg aagaggttct ccggaatctc
    43501 ggagtacccg ccgcatcgca tgacaaggac tccttggtcg ggcagctgaa tgatttcctt
    43561 caccgcctct ctcccgaaga aacgctggct ctggtcatcg acgaagccca cgaaatgagc
    43621 tccaaagtga tcgaagacct gcgccttctg ccgacccctg aaaacatcag ctcccgaaag
    43681 cttcaggtcc tcctggtggg gcagcccgaa ttaaaagatg ttctgaagtc tgaaagactg
    43741 aaggatttcc tggagaccat tgaagtccag ggagagctcc accccctgag cgaggaagaa
    43801 agccgcctct atatctccca ccgactgcag aaagcggaca gcgagatcag tcgagtcttc
    43861 accccggacg ctttggacct gatctgccag cacgctaaag ggaatccccg gaagctgaat
    43921 atcctttgcg acaactcctt tttgatcggt tacggccttt ccaagaaaaa ggtcgattcc
    43981 gccatcgttt ccgaggttct cgaggacttg gattttgcgg gtgaagaaga gcgaatcgcc
    44041 tggaaatcgg aagaatccct gaagcccaaa acactagcct acaggatcaa gaaccccctt
    44101 tttcgcaagc tggcttattc gctgcttgcc ctgtgcgggg tgggagccat catcctgctg
    44161 gggagaatgt acctgaagag tccggaagag acggtctctt cccgatttcc gatcaagccg
    44221 ccggtagtaa aagaaataag acccccggaa gcgaaacccg acttggcagc caaatcccct
    44281 cctcagccgg aggccgagaa aaagccttcc gccggcgttc ccgaaccttc ttcccccgca
    44341 attgccgaaa agaagccgtc cacacctcca tccggagggg aaacgaaatc tccggttccg
    44401 ctccctgtcg cggagaccgg gccggccccg aggaaacccg aatccccgcc cgttgctgga
    44461 gaaccgaaac cgtcagccaa ggccgaaccc aagtttgcag tcaccaagcc gattcccgtg
    44521 caggcttcag ggcccggagc aaaggaagag gcggtggcga aaaaaacagt attggttaga
    44581 ggaaaagata cgctttattc catcgccctt cggaattatg gcgttgccaa tacctccatc
    44641 gtagaccaaa tattggaagc caaccctaag attggcgacc ccaacaaact ctcggaccgc
    44701 cagagcgtcc gcctgccgga ggtccatgag gaatccttgc tcatcccgtc tgccgatggg
    44761 acctgccagg ttcgactggg gacatttttg aaggccgaat acgcggcttt tttgaaagga
    44821 gagccttctc tccggggaaa aaccgtagag gtcattccca ggaaattgcc ctcgggagaa
    44881 acctggtatc gagcggtagc cgggcagttc aacacccggg aagaagggtt gaaggttatc
    44941 caagaactta agagaaaggg tttatctccc tatttcccgg ggttcaggaa gaaaaaataa
    45001 tctcaagtcg ccagcagtaa gtcccagaat ggagcggaag tccaagggca tttttcccac
    45061 ctccagaaca aagtcccaat cctttttgaa tcagaagccc ttccgtccac cgcaagaacc
    45121 cgccggtttt tagctgcttg agttttttcc ccaaacttga aggccaggtc aagacccact
    45181 aagaggcgaa agaaacgaag ggccgcccgg gccgaaagga agaagattaa agaaaaaaaa
    45241 gaataagggc tatggcgttg aacgcagaga aggtgggaaa atgtcaccgg ggaaacctcc
    45301 gaagcgaggg aggaaagatc gaatcctctt gaaaaaaaac cccgggaaac ggataagaga
    45361 tgcaccaatg gatagaagat cagaatccaa ggctcggcgc aaggtaaagt aactcccgag
    45421 aagaagggag aagagatgac aaggaaggaa aggggataga gggctggggg cacttgatga
    45481 aaagattgga aagaaaaatt ttagaaaccc tcgcaatgca tgccaagaaa ttgagggaat
    45541 atacgggaaa aaggataaaa ccagggagag gaaatgaaaa aacaagtagg tcctccttat
    45601 ataaggcctt cgctggccgt ggtagagtta gcggaggtac gggtccttcg gggggcctgt
    45661 aaggcccctg tacgcagcgc tctttatttt ctgaaaacct cccgtccgat ttccgagaat
    45721 cagcaattca tatgccaatg ggcaaggaat gcgctcttgg ggtaatttgt taagatgatt
    45781 cgtgtttttt catcgagaaa gtgcctgtag ctttggtagg ttacaataat gggggaggaa
    45841 aagccaaaaa tggacatgga agacttttag tatggtgatg gcctgcccta cgacgcggaa
    45901 aggcagacaa tcctgggccc gagtctctta gaaaaatggg gttaacgact ttatgactaa
    45961 gtgacttgac acaacggcct cgatttctta cattggagga aaagaaatgc caacccaagt
    46021 tcatcacccc aactggcatg ggttcctcct gaacggacag accgtcgaaa ccctgccgat
    46081 ctttcgaacc attcaccgaa acggttgaga aagagagctc gcacggatga atcatcttcg
    46141 ggaagtggtg agaggcgtgt acctggtcgg gtctccagag ttgaccgccc ctgaagattg
    46201 ctgcgtatac cttctggacc tgggggacct ggttctgatc gacgccggtg cgggaaggac
    46261 cgtgcctgcc atccttgaaa atatcgcctc tctgggactg caccacaaaa ccctggccgc
    46321 tgtggtcgcc acgcattgcc atattgacca catcggaggc attcccgaaa tccgtcagag
    46381 gactggatgc cagatcatcg cccattcttt ggatgccccg gccatggaga agggggactc
    46441 ggggaagacc gccgccgatt ggtaccggat gacctttccg cccactccgg tggacatcaa
    46501 gttaaatgaa gtgcgggaag ttctgaaatt cgaaaaagga gaggtccttt gccttcacac
    46561 accgggtcac actccggggt ccatttctat cattctggac cgggaggggc aaagggtcct
    46621 cttcgggcaa gatatccacg ggcccttctc ggattccttc ggctcggacc tggcggcctg
    46681 gagaaagtcc atgggaattc tcctgggctt gaaagccgat atcctctgtg aaggtcattt
    46741 tgggatcgtc caaccggaga aagaggtgcg gcggtacatt caagactatc tggaaaggta
    46801 taaaaaagaa aattgatgaa ttggtggaaa gtcgaaattt cataaaactc gacactccgg
    46861 cgtaccccgg atcgggtccg gggcaggctc cggaaaccag tttttccaag atgttctaga
    46921 cctcgggttt caccggggtg acgattcaag agattgaaag accgtcaaaa gtcatcccga
    46981 aaataatttt atttgcgact ctaaatgagc aagaaccgat ccggaattgt gaccctaata
    47041 accgactttg gctggggtgg agaatacgtg ggggccatga agggggccat cctccgagta
    47101 aacccggaat gccgcgtcgt cgacctcact caccaggttg aaccccagaa cattctccag
    47161 gcggcctata tcctgaaaaa cacctttcca ttctttcctc cgggaacggt ccatctggca
    47221 gtcgtcgacc ccggggtagg ttcccggcgg aaggccatcg tcgtaaaaag agaagagcat
    47281 cttttcgtta gtccggacaa cggggttctc agcggtatct tgggggggaa agaaacggcc
    47341 tcagggtacg aaatcaagga gagaaaatat tttctctctc cggtgagcga tacgtttcat
    47401 ggccgagaca tctttgctcc tgttgccggg catttatctc tgggcctaga cccctcaaag
    47461 ctcggcccgc agattcaggg cctgaaaacc attgtcttgc cccggcctcg aatggtcaaa
    47521 ggaggattga gggcccgcat cctctgggct gattctttcg gaaacctgat taccaacctg
    47581 gcccgcgagg attacggccg gaaattggaa acccgtccct ttcaaatcaa ggggaagggc
    47641 tggaagatcg accgggtcta ccggacctac ggcgaggggc tcccgggaca gcccatggcc
    47701 ctcttcggga gcggaggatt tttggaaatt tccgtgaacc ggggaaatgc ccggaaaacc
    47761 ttgggcctta aaccaggaga ccctattatt atcctatggc gataagaatc ttgagagaca
    47821 atccccgttt gggtattccc tccttcttcg ttctgatcac tcttcttttt cccgggggcg
    47881 atcttccggc ggctccttcc ctccctgggc agttcgagca aggggtcctg ctttttaaaa
    47941 agggggaata ttccgaggcc ttgaaaattt tccaaggttt ggaagaagcc tccaaagaac
    48001 cttcttctac tgccgactta gccttcatgc aggggcaggt ctttcgggaa ctgcagaatt
    48061 ggcctgaggc ggctcaggct ttctcccggg cagctgaaaa ccatccgctg ctcgccgact
    48121 atgccctctt ctttcaaggg gagtccttgc agaaaatgag cgaaggagag aagagcctgg
    48181 ccgttttccg ccgccttttt gacctttatc cccagagcct cgtgaatacc caggcccggt
    48241 tgcggaccgc cgagatctac ttccagtccg gggacttcca gaatacggcc gaggtttgtg
    48301 agctgattct gcaggggagc ccctggaagg atatccctgc ccaggcctgg ttccttctgg
    48361 ggcaggcccg ggaagggttg aaccaatatt cggaggcgat ccaggcctac caggaaactt
    48421 ggatgaaata tccccttcat ggggcagcga agaaagcccg cacccgatgg gaggccttgg
    48481 cccaggaaaa gaaagtggcg gtgaaaaaga tatccactga agcgctcttc aaccgcgccc
    48541 tccaattcta ccaagcccat cagtatgaaa ctgccctgaa ggaaatgaat cggatcgagg
    48601 gtcttcctgc acagagttat cctgccactt atgctgggga actgtgggtg gacgatctct
    48661 actatcaccg gggaatgagc tacttccgcc tgaaacagta ctcgaaggcc gtggagaacc
    48721 tggacctcgt ggtccgctct tcgcgaaacg aaggaatggc cgagaagagc ctcttctccc
    48781 tctttcagac cttgacccgc agcggccgca gggaagaggc gctgaggatt catgatttgt
    48841 tccaggcctc ctacccccgg agttcctttc tgcctcaagc tctgtacctg aaagccagcc
    48901 tgtatgaaga ccggggggaa cccgagaagg cggtgtctct ttacctgagc gtggcggacc
    48961 aattcctcca aagccctttc cgttttccgg ccctatggaa cgcgggctgg gtccagtacc
    49021 agaaaaagga ctgggccgca gcccttcagg catgggaaag gttgaagaag gcagaacccc
    49081 atttccggtg gatggaaaag gcgctgtatt ggagtggcaa agcgctggaa aagatcgacc
    49141 ggaaggaaga agcagaggaa cagtaccagc aacttctgag ggagtacccg gcgtcctatt
    49201 acagccagtt gatctcatcc cggggactgg agccccgggc ttctccaggg ccggtcccaa
    49261 ttctccagga ccgggttctc tcctcggtcg aggaaatgaa aaccaattcc cccggcccca
    49321 agtcgattca tctggaaaag gggagactcc tggcccgttt ggccctgagc ccctgggcca
    49381 tcgacgaact ggaggccgcg gagggagaag ggggatccct ggatgaaaaa tggatcgaag
    49441 tctcccgtct ctaccgggaa agcgaggagt attaccgctc caatcttctg gtccgcaaga
    49501 agtttgctct gaaacccttc tccggcagtt cctccgaaaa agaaaggact ctctacctgc
    49561 ttgcttatcc tctggggaat tcctccctgg tcaatcgctt ttctcaggcg tgcaatcttg
    49621 accccgccct tctttccgcc ctcatcctgg aggagagtcg ctttcaggtc caggcggtct
    49681 ccccggcagg tgcccggggg ttaatgcagg tcatgccgca gaccggcagg caaatcgcca
    49741 aggaactgaa ggtgcgcccc ttctccgagg atcagctctt cgacccggcg gtcaacatcc
    49801 gtctggggag ccggtatttc gccaagatgc tggaggaatt cgaaggaaag gtccatctgg
    49861 ccctggctgc ttacaacgca gggccccagg cagttcggaa atggctggca agcagccttt
    49921 cttcggcgga agatgagttt gtggagaaca tcccctattt ggagacccgg aattatgtca
    49981 tccgggtcat cggcagcgcc caggtctatc ggttgcttta ctgcccgcaa cccgatagcc
    50041 cagcccagcc ctgattcacc ttcagcggaa ccgcttcccg ctcgtggcca gccaggtagt
    50101 tgatcgggtt gaccgagtcc cacgccgccc gcttcatgga aatcaggtga gaaatggaaa
    50161 aggtatgacg caggatggca gctgcgcctt cccagccgat ctgcgaccag gcgtagcccg
    50221 tcttatagag ttcggtgaac tcccggtaaa aatcggccag cttcagcttg gtggggagaa
    50281 cagcgtgaac gtaatcgaag agctcgtaat tggtggtgac cagcttttct ttgacccggg
    50341 caaagaggtc cgtccctgga agaggggtca ggatggtcag ggaaggagaa tacagcttcc
    50401 ggtccaggat atactggcgc aagcgctgaa aatcctgctt ctggaaggaa ggatcgatga
    50461 taaaggcggc gatcacctcc attccatgtg cccggaggac cttgagagcc tcctcgttat
    50521 tctgcaccgt gttatgcttg ttcagctggc tcatggcctc ttcgtcgatc ttttcaaaac
    50581 cgatgaagac tttccagaat ccggcttttc gaaggagagg gatgacctcc gggtgacgga
    50641 cgatggcatc gctccgggct tgaaaagtaa agcgtttctt aatcctgctg tcaatcagga
    50701 gctgggcgat ccgcttggcc cggggcacgc tctggagaaa gttgtcgtcc gccaagaaga
    50761 tgtatttctc cgggatggtt ttcacttcct caaggacccg ctcggggctt ttcatccggc
    50821 atttcccgtt gaagaatttc cacacgctgc aaaaatcaca gcggtaggca cacccgcggg
    50881 cggtctccat gatggagaag ggcttctgga accccaggta ataatgtttg cggtaatgcc
    50941 gggtcaggtt ccgggcggga atggggattt cgtccaggtt ttccaccatg gggcgctccg
    51001 gggtgtaaaa gggggcggag ggctcgttga tcaccaggcc ggggagggtt ttcaggtccc
    51061 gtccctgttc gcaggctcgg accaggtcgc ggatggcagc ttcgccttcc cccaccacta
    51121 ctccgtaaat atccgaacag tagaagtcct ccggagagag cgaggcatgg tgccccccga
    51181 cgaaaacagg aatcctcggg ttccatttct tgatggtggt ggctatttcc cgcgcctggt
    51241 agacgtcgat ggtgaaagaa caattgatgc cgcagaaatc cggctggaat tcctccagga
    51301 cccggggcag gcgattctcg atacgcaagt ccagaatctt tacgttgtgc tcgggcaggt
    51361 tcgcggcgat ggactccagt cccagcggct ccaccacggc caggctgcgg aatcccaagc
    51421 cccgctcttg cgagggctgc ataaaaagaa ttttcatttt tcgcttcctc aggtggcggc
    51481 ggaatatgta aaaggtcccc tgtcacccac ttacaaggga cctttttcga ctggcggggt
    51541 cgacgggatt cgaacccgcg gcctccggcg tgacaggccg gcgttataga ccagcttaac
    51601 tacgaccccg gtaggcggaa acatttaaaa aaaatatttt aaaaatcaca tattgtcaag
    51661 ggtttttttg cggcctatgg ggtactggcc accagaacct ctaccgcgcc tgatgtttga
    51721 aaatacccct aatatttgac ggttacgctc tgcagaaggg gttcccatta cacattcagc
    51781 agctcactgg ggttttttgg gaccattatc tgaatctctt ccccggttat cccttgggtt
    51841 aatagagaag cgataaacat ccgcatcatt tctgaaggaa gggaggccca atcaaagaat
    51901 gaatctgttg tgagaaccaa acgctctgct ccaatctcct tgacccttcg gcacatttcc
    51961 tggatactga ttctgcaaag ggcggggagt
//
LOCUS       DASPRR010000123        12466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1479516, whole genome shotgun sequence.
ACCESSION   DASPRR010000123 DASPRR010000000
VERSION     DASPRR010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12466)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12466
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479516"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VEL68_04480"
     CDS             <1..663
                     /locus_tag="VEL68_04480"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0016860 - intramolecular oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HXZ35259.1"
                     /translation="KETVYGEDPGAMAEKWVSQGARFLHLIDLDGAREGVPQNRITIQ
                     AIARKSPVPVEVGGGIRVLSTVEDYLSSGIDRVILGTAAYADPDFLKECCDRWPGRIA
                     VDIAAKAGKAAVSGWTEATRISAADLARKCEELGASVIIYTDIERDGTQVGVNLRDTR
                     EVARSLKVPVIASGGVSTLEDIQALLPLEKDGVKGVIVGRALYAGTLNLSDAIRITEE
                     KV"
     gene            665..1504
                     /gene="hisF"
                     /locus_tag="VEL68_04485"
     CDS             665..1504
                     /gene="hisF"
                     /locus_tag="VEL68_04485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HXZ35260.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGINFIDLRDAGDPVGNARVYDQQGA
                     DELTFLDITASHERRKILLEVVRRTADEVFMPLTVGGGIRTLDEIRELLQAGADKVSL
                     NTAAVQKPHLVREASEKFGSQCIVVAIDAKRRPSGEGVWGQADQMARDGMENELLGLG
                     KRVSWEVYIHGGRTPTGIDALDWARKVEEWGAGEILLTSMDRDGTKEGYDIPLTRIFS
                     DSLSIPVIASGGAGTIEHLLEALIQGRADAVLAASIFHFRQHTVQEVKAYLKRRAVLV
                     REA"
     gene            1770..1967
                     /gene="rpsU"
                     /locus_tag="VEL68_04490"
     CDS             1770..1967
                     /gene="rpsU"
                     /locus_tag="VEL68_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXZ35261.1"
                     /translation="MPGVRVKEGESFENALKRFKKQCEKAGILSEIRKREHYEKPSVK
                     RKKKALAARKRAMKRMRIYED"
     gene            1974..2432
                     /locus_tag="VEL68_04495"
     CDS             1974..2432
                     /locus_tag="VEL68_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXZ35262.1"
                     /translation="MTIKEQIAHDYQAALKARDERKLSALRMVRTEAKKREVSGQKKE
                     LSDAEVLETISSLVKQRRESIRLFAEGKRQDLVEKEEAELKVLLSYLPQPLSTAELEG
                     LINQAIQETQAAGPKDQGKVMKWIMPQVSGRADGKVVSEMVKQKLAQPAS"
     gene            2722..5046
                     /locus_tag="VEL68_04500"
     CDS             2722..5046
                     /locus_tag="VEL68_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXZ35263.1"
                     /translation="MEERSLRVLEFHLFLQGLQTYASSEVGQALCMELRPVSRHGEVQ
                     RRLQEVSEAQDLLEMDGDIPLEGLGELRPILHQARAEGTCLPPQSLLRVQGTAVAAGR
                     VRGFLQKSERPHPRLKEWEEKVPELRDLHEALRSAIGPRGEILDSASPELRRLRREVS
                     LLRARIRRALENLWEREDLHKVFQEQIITLRNERYVVAVKAEFKNTLPGIIHDQSQSR
                     ATFFIEPLSTLEENNELNMLLQDEKEEERRILLFLTDRVREKSKEISRAVEVLGHLDL
                     VFAQAKWARAFRGIIPALNDRGCWRLRGARHPLLGSKAVPIDIHLEPGQSTLILTGAN
                     AGGKTAALKTLGLLTLIAQSGVPIPADEGSEVAVFDKVFADIGDEQDLRENLSTFSAW
                     VRSVAHILQQADESSLVLLDEVGGGTDPMEGAALTMAILDGMHSRGVKVMVTTHLHLL
                     KAYGAINPHVANVSVEFNPETLRPSYRLIYGLPGESHALLMAQKWGLPAEVVQKAYGY
                     LGDGERKTSQLLQSLEQTQREMEKKLQEGEMIKREAEAFRRQSETLLRQTRQEEERLM
                     AQVREESRAVVQHARAELRLLINEFKAKGRTDLHRLGQEIQAVEERISGWARKKGEEV
                     RPWERPETAPSSSDSPDATLDRLKEAFAQRSGKESLRPAKRGRIDYPIPPGKREVKVI
                     GLRVEEAIPLVDKAIDEAFLCGLKELEVIHGAGTGRLRQAIREHLQDHPLVKAFLPGG
                     PGRGGNGVTVADIGPAPSSRPAQRRSNKIGAEGG"
     gene            5050..6909
                     /gene="dnaG"
                     /locus_tag="VEL68_04505"
     CDS             5050..6909
                     /gene="dnaG"
                     /locus_tag="VEL68_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXZ35264.1"
                     /translation="MSDLIPEGKIDEVRQRASIIEVVSDFVSLKKTGKNHLGLCPFHS
                     ERTPSFTVNEEKGIFHCFGCGAGGNVFNFLMRASQMSFPETVRALAKRYGVPLPTREL
                     SEEEKRRRSLRGKLYEVNETAADYYHRTLTSLKEGEEGRQYLAQRGVSEETIREHRLG
                     FAPPGWDSLVLFLQKNGTPLNLAETVGLILPRKEGTGSSGRPGFFDRFRRRVMFPIIS
                     EGGRVLGFGGRVIAEDKGEGELPPPKYMNSPESPIYRKGQTLYGLNAAKGFIREKGNA
                     LIVEGYMDLLSLYQAGFRNGVASLGTALTSSQLQLLGRYTREVFLIFDADESGEKATQ
                     RSLDLFLQEGISAKVVTLPAGYDPDSLIRREKAERFEQVLAEALPLVEYLFQQTLRRH
                     GAGSVEGKVRIVREMIPALSRLADPLERNLYIERIALRLGLKESQVRDQFRGGEPVEA
                     ESEKLPPSAHRGPAHERVLLQLMIVNPAVISRVQEVMGKEGFSDLRHQKLAGKLLELG
                     EGREEIGIQEFLSGTEEEDLRDLVSELLLEEESLVDPDRMLEDCLRKVKLSRIRQEIQ
                     RVDEEIRQRSREKKDEPWGIPGLKELLMRKQRLLLEQKRWVGETPGRLQAQEG"
     gene            6937..8685
                     /gene="rpoD"
                     /locus_tag="VEL68_04510"
     CDS             6937..8685
                     /gene="rpoD"
                     /locus_tag="VEL68_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXZ35265.1"
                     /translation="MAKEEEMEKVKALIHMGKKKGFLTYDEVNNLLPPDIVSSDQIDD
                     LMMMFGEMDIEVVDAAHKVKVSKQREEVEALPPEEELELDAEGLGKSIDPVYMYLREM
                     GSVSLLTREGEVEIAKRIEQGEKEVIKSVLDSPLTIKEIIQLGERLRSKKVKPKELAP
                     ELEDEDEYFLEEEFNVQRILALIDQIRKLDERNRQLREKLPKAGASARPHTEKKLREN
                     KEKIVSLLKDINLKTKHIDKIVQKLKGLAERVEKAEGEIHEVLSLAHLPLDQLQRVLR
                     MMKKKPGEFRKFLRKSTLKKELLEEYDRVIRNAHRKVRRVEAESNLPVGELKRTLESV
                     RNGEVKAEVAKNELIKANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQR
                     GYKFSTYATWWIRQSITRAIADQARTIRIPVHMIETINKLIRTSRYLVQEFGREPTPE
                     EIAERMDFSLDKVRKVLKIAKEPISLDTPIGEEEDSYLGDFIEDKKVVAPSEAVVQLN
                     LSEQTRRVLSTLTPREEKVLKMRFGIDEKADHTLEEVGQNFDVTRERIRQIEAKALRK
                     LRHPSRSKKLRAFVEG"
     gene            8773..8848
                     /locus_tag="VEL68_04515"
     tRNA            8773..8848
                     /locus_tag="VEL68_04515"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8806..8808,aa:Ile,seq:cat)
     gene            8979..9707
                     /locus_tag="VEL68_04520"
     CDS             8979..9707
                     /locus_tag="VEL68_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HXZ35266.1"
                     /translation="MRNQLEKLIILQTIDLRVLQMEREKSLIPQSIASLEEELKKEEE
                     KFRLEKEGHEKLQKERRKKEKDLEEEVDKVKKAEARVLEIKTNKEYQAVLKEIDNAKK
                     LNRQREEEILDILEKLEETQKRITRGEKDLDEKRKEMERQVAVLRQKESSFEQEMAGK
                     VQQREQKEKEITPELLSRYRMLAEKRQGIAVARVVQGVCQACHMNLRPQLYIELQKQD
                     SLIICPNCSRILFWDNGAPKAEKQ"
     gene            9704..10348
                     /locus_tag="VEL68_04525"
     CDS             9704..10348
                     /locus_tag="VEL68_04525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXZ35267.1"
                     /translation="MREDPPPDSPRLFEEKKETSPSSEGRILRHLSQTLSVTKTLKRF
                     PSLTPQYLQEVLQRCADRAEEDRKAPPPDLQTGEFPEFFIHADGASRGNPGEAGAGAV
                     ISDSQGRTLKELKAYLGIASNNGAEYQAAILALERALELEVKRVTLFLDSELVVRQLR
                     GEYRVREPHLKSLHQKARETLNRFSQYSILSIPREENRRADQLANEAIDQKIKE"
     gene            10442..10815
                     /gene="rnpB"
                     /locus_tag="VEL68_04530"
     ncRNA           10442..10815
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEL68_04530"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            10982..11509
                     /locus_tag="VEL68_04535"
     CDS             10982..11509
                     /locus_tag="VEL68_04535"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HXZ35268.1"
                     /translation="MPMYEFEGKRPRVGETSFVHPCAVLIGGVTIGENCYIGPGAVLR
                     GDFGYIEIGDGSNVQENVVIHTFPEQTARLAKDSHIGHSAIVHGSKIGENVLIGMGAI
                     LHEGVEIGKDCIIGSGCVITDNRKIPPGKLMVGVPGKIIADVSPEMTRAKVAGTRLYQ
                     TLPRRYRETMRLIES"
     gene            11600..11770
                     /locus_tag="VEL68_04540"
     CDS             11600..11770
                     /locus_tag="VEL68_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35269.1"
                     /translation="MFLVPADQAKEWLAKGMRLFTFQNDVRMLMDAGKANTAQLREFI
                     GKKGEGFSGTES"
     gene            complement(11793..>12466)
                     /locus_tag="VEL68_04545"
     CDS             complement(11793..>12466)
                     /locus_tag="VEL68_04545"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HXZ35270.1"
                     /translation="QTMKKKKSLVIACNISKEELLHVQSDEASFIFLEQSLHRTPQKM
                     KGAIQEEIDKTETWDGDQIILAYGLCSNGILGVRANRHPLLIPKVHDCISLFLGSNAR
                     YMEEHQREPGTYYLTKGWIEEKKSPLGIFEEYCQRYGKETAEWVIREELKNYTRIALV
                     ESELGMEETHRQHARENARFLNLKYEEIKGSLDFFRKIIRGPWDKDFVVLRPGEEATQ
                     EMFLE"
BASE COUNT         3010 a         3313 c         3756 g         2387 t
ORIGIN      
        1 aaagagaccg tgtacgggga agaccccggg gccatggccg agaaatgggt ttctcagggc
       61 gcccggttcc ttcatctgat cgacctggac ggcgcccggg aaggagttcc ccaaaaccga
      121 attacgatcc aagccatcgc caggaagtct ccggtaccgg tggaagtggg cgggggaatc
      181 cgggttcttt caaccgtcga agactatctc tcttcgggaa tcgaccgggt gatcctggga
      241 accgccgctt acgccgatcc cgatttcctc aaggaatgct gcgaccgatg gccgggcagg
      301 atcgcggtag atattgccgc aaaggcgggg aaagcggctg tctcaggctg gaccgaggca
      361 acccgcatat ccgctgcaga cttggcccgg aaatgcgaag aactgggggc ctcggtcatt
      421 atctacaccg atattgagcg ggatggaacg caggtgggcg ttaacctgag ggatacccgg
      481 gaagtggcca ggtccctgaa ggtcccggtc atcgcttcag gcggggtctc caccctcgag
      541 gacattcagg cccttcttcc cctggagaag gacggggtga agggggtgat cgtgggaagg
      601 gccttgtatg cgggaaccct aaacctttcc gatgccatcc ggatcacgga ggagaaggtc
      661 tgaaatgctg gccaaaagaa ttattccctg cctcgacgtg aaggacgggc gagtggtaaa
      721 aggaatcaat tttatcgatt tgcgcgacgc gggcgatccg gtgggaaatg cccgggtcta
      781 cgaccaacaa ggagccgacg agttgacctt tttggacatc accgcctccc atgaacggcg
      841 aaagatcctc ctggaggtgg tgcgccggac cgccgatgaa gtattcatgc ccttgaccgt
      901 gggaggagga atccgcaccc tggatgaaat ccgggagctt cttcaggcgg gagccgacaa
      961 ggtctccctg aataccgctg cggtgcagaa gccgcacctt gttcgggaag cctcggaaaa
     1021 attcgggagt cagtgcatcg tggtcgctat cgatgccaaa cgcaggccgt cgggagaagg
     1081 ggtttggggc caggccgacc aaatggcccg ggatggaatg gagaatgaac tccttggcct
     1141 ggggaaacgg gtctcttggg aggtatacat ccacggaggc cgtaccccga cgggcatcga
     1201 tgccctggat tgggcccgga aggtggaaga gtggggtgcc ggagagatcc ttctcaccag
     1261 catggaccgg gatgggacaa aggagggata cgatatcccc ctgacccgca ttttttccga
     1321 ttcgctgtcc attcccgtca tcgcctcggg gggggctgga acgatagaac atttgctgga
     1381 agccctgatc cagggtcggg ctgacgcggt tttggcggct tccatctttc atttccgcca
     1441 gcatacggtc caggaagtaa aggcctactt gaaaagaaga gcggtcttgg tgcgggaagc
     1501 ataatcatat tcatcggttg gaatcttctc agggtttcgc ccggcgaatc aatccaaaac
     1561 aaaaaaatcc tttgactttt tagatatttt ccattaaaat ttattatttc gggccatatg
     1621 ccgcaagcga agtcaatcga gcacgtggta aagcttttcc tgcggcattc tctgtgattt
     1681 tcaaaatctt tttccgaaaa tgatgttatt ggtcggtgat ttcatatata ttctaaaatt
     1741 tccggcagtc cggagggagg cgagtatgaa tgccgggggt cagagttaaa gaaggagaat
     1801 ccttcgaaaa tgccctgaaa cgctttaaga agcagtgcga aaaggccggg attctttccg
     1861 agatccgcaa gcgggagcac tacgaaaagc ccagcgtgaa aaggaagaag aaggccttgg
     1921 ccgcgcggaa gcgggccatg aagcgcatgc gcatttatga agattaatcc agcatgacga
     1981 tcaaagaaca gatcgctcac gattaccaag ccgcgctgaa ggctcgggat gaacgcaagc
     2041 tctccgcttt gcgaatggtt cggaccgaag ccaagaagcg ggaagtcagc gggcagaaga
     2101 aagaactatc ggacgccgag gtcctcgaga ccatctcttc cctcgtcaaa cagaggcggg
     2161 agtccatccg cctctttgcc gagggcaagc gccaagactt ggtggaaaag gaggaagcgg
     2221 aactgaaggt tcttctctcc tatcttcccc agccgctgtc aactgccgag ctcgagggtc
     2281 ttatcaacca ggcgatccag gaaactcaag ccgccgggcc caaagaccag ggaaaggtca
     2341 tgaaatggat catgccccag gtgagcggcc gggcggacgg aaaagtagtc agcgaaatgg
     2401 tcaaacagaa gttggcccaa ccggcgagct gaagaccctc ttcacgggct cgcatccata
     2461 gataatcccg aggccctgac aacgcgtact ttgcattctc ggaatcgagc gggcaactcc
     2521 ttaggtcaca cgggtaaacc cttttgcggc cgcggagcgc agggacggcg gacattctta
     2581 ttccagccct cacctttcga tcagcgaaca ttctctgccg tttattctgg actttccctt
     2641 ttcggggaag cggccttgga gtctctttgg aaaaagggag agagcgctga agctgttctt
     2701 tcagcggatt agagcaagtc catggaggaa cggtcactcc gcgttttaga gttccatctt
     2761 tttctgcagg gcttgcaaac ctatgcttcc tccgaggtgg gccaggctct ttgtatggag
     2821 ttgcgccccg tttcccgaca tggggaagtg caaaggcggc tgcaggaggt ttccgaagcc
     2881 caggacctgc tggaaatgga tggagacatt cccctggaag ggcttgggga attgcgcccc
     2941 atcctccatc aggcccgggc tgaaggcacc tgcctgcctc cccaaagtct cctccgggtc
     3001 caaggtacgg cagtagcggc cggcagggtc cgagggttcc tgcaaaaaag cgaacgtccc
     3061 catcctcgcc tgaaggaatg ggaagaaaag gtccccgaat tgagggacct gcacgaggct
     3121 cttcgatcgg ctatcgggcc ccgcggcgag atactggatt cggccagccc cgagcttcgc
     3181 cgcctccgcc gggaggtctc ccttttacgg gcgcggatcc gccgcgccct ggagaatctc
     3241 tgggaaagag aggacctgca caaggtcttt caggaacaga tcatcaccct gcgcaacgaa
     3301 cgctacgtgg tggcggtcaa ggctgagttc aagaacaccc tcccggggat cattcacgac
     3361 caatcccagt cccgggccac ttttttcatc gagcccctct ctactctgga ggagaacaac
     3421 gaactcaaca tgctcctcca ggatgaaaaa gaggaagaac ggcggattct ccttttcctg
     3481 accgatcggg tccgagaaaa aagcaaggaa atcagccgcg cggtagaggt tctgggccat
     3541 ttggacctgg tcttcgccca ggccaaatgg gcgagggctt tccgggggat tatcccggcg
     3601 ctcaatgacc ggggatgctg gcgactccgg ggtgcccggc atcctctgct ggggtctaag
     3661 gctgttccca tcgacatcca tctcgaaccg gggcaaagca ccttgatcct taccggggcc
     3721 aacgcggggg gcaaaacggc cgccctcaag actctgggcc ttctcaccct gatcgcccag
     3781 agcggggtgc ccatccccgc ggacgagggg agcgaggtgg ccgtcttcga caaggtcttc
     3841 gcggacatcg gcgacgagca ggacctacgg gaaaacctga gcactttttc ggcttgggtt
     3901 cggtcggttg cccacatcct tcagcaggct gatgaatcct ccctagtgct tctggacgaa
     3961 gtggggggag gaaccgaccc tatggagggg gccgccctga ccatggccat cctagatgga
     4021 atgcatagcc ggggagtgaa ggtcatggtt acgactcacc tccatcttct caaggcctac
     4081 ggggccataa acccccacgt ggccaatgtt tcggtggaat tcaaccccga gaccctccgc
     4141 ccatcttacc gtctcatcta cgggctcccg ggagaaagcc atgccctgct catggcccag
     4201 aagtgggggc ttccggctga agtcgtccaa aaggcctatg gctacctggg cgacggggaa
     4261 cgcaagacca gccaactcct ccagagcctg gagcaaaccc agcgggagat ggaaaagaag
     4321 ctgcaagagg gggagatgat caagcgcgag gccgaggcct tccgcaggca gagcgaaacg
     4381 ctgttgcgtc aaacccggca ggaagaggag cgcctcatgg cccaagtccg ggaagaatcc
     4441 cgggccgttg tgcagcacgc ccgagcagag ctgcggctcc tgatcaacga gttcaaagcc
     4501 aagggtcgaa ccgacctgca ccgccttggc caggaaatcc aggccgtaga agagaggatc
     4561 agcggctggg cacggaaaaa aggggaggag gtcaggcctt gggagaggcc ggagaccgcc
     4621 ccgtcttctt ccgattcgcc ggatgccacc ctggaccggt tgaaagaggc ttttgcccaa
     4681 agatcgggga aagagtcgtt gcggccggct aaacgggggc ggatcgacta cccaattcct
     4741 ccgggcaagc gggaggtcaa ggtcattggc ctgcgcgtgg aagaagccat tcccctggta
     4801 gacaaggcga tcgacgaagc cttcctttgc gggctgaaag aactggaggt gattcatgga
     4861 gcgggtacgg gccggttgag gcaggccatc cgggagcatc tccaagatca ccccctggtt
     4921 aaagcctttc ttcccggcgg cccagggcgc ggaggaaacg gagtgaccgt agcggacatc
     4981 ggtccggctc cttcttcccg tccggcgcag cggcggtcga acaagatagg ggccgaaggg
     5041 ggataaacga tgagcgacct catccccgaa ggaaaaatcg acgaggtccg ccagcgcgcc
     5101 agtatcatcg aggtggtctc tgatttcgtc tccctgaaga agacgggcaa gaaccacctg
     5161 gggctctgcc cgtttcactc cgagagaacc ccctccttca cggtgaacga ggagaaggga
     5221 atcttccatt gtttcggctg cggggccgga ggaaacgtct tcaattttct gatgagagcc
     5281 agtcagatga gttttcctga aaccgtccgg gctttggcca agcggtacgg cgtgcccctt
     5341 cccacccggg aactttcgga ggaggagaag cgtcgccgga gcctgcgggg aaagctgtac
     5401 gaagtgaatg agacggcggc cgattattac catcggactc taacctcgtt gaaagagggg
     5461 gaagagggcc gacagtattt ggcccagcgc ggggtctccg aagaaactat ccgggagcat
     5521 cgattgggct tcgcccctcc tggctgggac tccctggtcc ttttcctgca gaaaaatggg
     5581 actcccctga atctggccga aaccgtgggt ttgatccttc ctcgaaagga gggaacggga
     5641 tcatcaggac ggcccggctt ctttgaccgt tttcgtcgac gggtcatgtt ccccatcatc
     5701 agcgaagggg gacgagttct aggatttggg gggcgcgtca ttgcggagga taagggagaa
     5761 ggggagttgc ctccgcccaa gtacatgaat tcgcccgaat cccctattta ccgcaagggc
     5821 cagacccttt atgggctgaa cgcggccaag ggattcatcc gcgaaaaggg aaatgccctg
     5881 atcgtggaag gctatatgga cctgctctcc ctataccagg caggcttccg caacggagtg
     5941 gcctctctgg gaacagcgct gacctcttct cagctccaat tgctggggcg atacacccga
     6001 gaagtgttcc tgatctttga cgccgacgaa agcggggaaa aagctacgca gcgcagcctg
     6061 gaccttttcc tgcaggaggg gatctcggcg aaggtcgtca ctttacccgc ggggtatgac
     6121 ccggacagcc tgatccgcag ggagaaagcg gaaaggttcg aacaggtgtt ggccgaagcc
     6181 cttcccctgg tggaatacct ttttcagcag actctccgcc ggcacggggc cggttcagtt
     6241 gaggggaagg tccggatcgt ccgggaaatg atcccggcac tcagtcgact ggccgatccg
     6301 ctggagagga atttgtacat cgaaaggatc gctcttcgct tagggctgaa agaatcgcag
     6361 gtccgggatc agttccgggg aggggaaccg gtcgaggcgg aaagcgagaa gcttcctccg
     6421 tccgcccatc gcggcccggc tcacgagcgg gtgctcctgc aattgatgat cgtgaacccg
     6481 gcggtcatct cccgggtgca ggaagtgatg ggcaaggaag gtttttccga ccttcgccat
     6541 caaaagttgg ccggcaaact cctggagctc ggggaaggga gggaggaaat cggcatccag
     6601 gagtttctca gcggaacgga ggaggaagac ctccgggacc tggtctctga acttctcctg
     6661 gaagaggaga gtctggttga cccggaccgc atgctggagg attgcctgcg aaaggttaaa
     6721 ctctcccgga ttcgacagga gattcagcgg gtcgatgaag agatccggca gcgaagccgg
     6781 gaaaagaagg acgaaccctg gggaataccg gggttgaagg aacttttgat gaggaaacag
     6841 cggctccttc tggagcagaa gaggtgggtg ggggagaccc ccggccgcct ccaggcccag
     6901 gaagggtaag gagccccagg cttggtgagg ggaatgatgg ccaaagaaga ggaaatggaa
     6961 aaggtaaaag cgctgatcca catgggaaag aagaagggct tcctgaccta cgacgaagtc
     7021 aacaatcttc ttcccccaga tatcgtatcc tcggatcaga tcgacgatct aatgatgatg
     7081 tttggagaga tggacatcga agtcgtcgat gcggctcata aagttaaggt gagcaagcag
     7141 cgggaagagg tggaagcgct gcctccggaa gaagagctcg agctggatgc cgagggcctt
     7201 ggaaaatcga tcgatccggt ctacatgtac ctgcgggaga tggggtcggt gtcccttctg
     7261 acccgggaag gcgaagtgga gatcgccaag cgcatcgaac agggagagaa ggaagtcatc
     7321 aaatctgtcc tggattcccc gctgacgatc aaggagatca ttcaactggg ggaacgactc
     7381 cgttccaaaa aggtaaagcc caaggaactg gctcctgaac tggaagatga agacgaatat
     7441 tttctggagg aggagttcaa cgtccagaga atcttggcgc tgatcgatca aatccggaag
     7501 ctcgatgaaa ggaaccgcca gctccgggaa aaactgccca aagccggcgc atcggccagg
     7561 ccccatacgg agaaaaagct gcgggagaac aaggaaaaaa tcgtctccct gctcaaggac
     7621 attaatttaa agacgaagca tatcgataaa attgtgcaga aactcaaagg attggccgaa
     7681 cgggtggaga aggccgaagg agaaatccat gaagtcttga gcctagctca cctccccctg
     7741 gaccaactcc agcgagttct gcggatgatg aagaagaagc ccggggagtt ccgcaagttc
     7801 ctccggaaat ccaccttgaa aaaggagttg ttggaggaat atgaccgggt gatccgcaac
     7861 gcccaccgaa aggtccgccg ggtcgaagcg gaatccaacc tgccggtggg ggagctcaag
     7921 cggaccctgg agtctgttcg caacggggag gtgaaggccg aggtcgccaa gaacgaattg
     7981 atcaaggcca atctccggct ggtggtctcc atcgccaaaa agtataccaa ccgggggctg
     8041 cagtttcttg acctcatcca ggaggggaac attgggctga tgaaggcggt ggacaagttc
     8101 gagtaccagc gggggtacaa gtttagcacc tatgcgacct ggtggattcg acagtccatc
     8161 acccgggcca ttgccgatca ggcccgaacc atccgaattc cggtgcacat gatcgagacc
     8221 atcaacaaat tgatccgcac ctcccgctac ctggttcagg agtttggccg ggaacccact
     8281 ccggaagaga tcgccgagcg gatggacttc tcgctggata aggtccgcaa ggttctgaag
     8341 atcgccaagg aacccatttc tctggacact cccatcgggg aagaagaaga tagctacctg
     8401 ggagatttca tcgaggataa gaaggtggtc gccccttccg aggccgtggt ccagctgaac
     8461 ctatcggagc agactcgaag agtcctgtcc accctgactc cgcgagaaga gaaggttctg
     8521 aagatgcgct tcgggatcga tgaaaaagcc gatcacaccc tcgaagaagt gggccagaac
     8581 tttgacgtca cccgggaaag aatccgccag atcgaggcca aagccctgag gaaactgcgg
     8641 catccttcga ggtcgaagaa actgagggcc tttgtggagg ggtgaggttc tgcagtaggg
     8701 gcgaccgggc cggtcgcccc tacaaatctt gacaagggcc cgggaaacag atatttttag
     8761 atagttagaa aggggcccat agctcagcgg gaagagccac cggctcataa ccggaaggtc
     8821 cctggttcga acccaggtgg gcccaccaga ttgtccaaaa tgacgatttc aagtacatct
     8881 gagccagcaa aaatgggaag gcctctggaa aggaccagca gggccgaaat taaggagtgc
     8941 accccccggg gtgcatttct tttttcagga gggggaaatt gcggaatcaa ttggaaaaac
     9001 taattatatt gcagaccatc gacctgcggg ttcttcagat ggagagagaa aaaagcctga
     9061 tccctcagtc gatcgcttct ctggaagagg agttaaaaaa ggaagaggag aagttccgcc
     9121 tggagaaaga gggccatgag aaactgcaga aggagcgccg taaaaaagag aaggacctgg
     9181 aggaagaggt cgacaaggtg aagaaagcgg aggcgcgggt cctcgaaatt aagaccaata
     9241 aggaatatca ggccgtcctg aaagaaattg acaacgccaa aaagttaaac cgccagcggg
     9301 aagaagaaat cctcgatatt ctggagaagc tcgaagagac gcaaaagcgg attacgcgcg
     9361 gggagaagga cttggacgaa aaaaggaagg agatggagcg gcaggtcgcc gttttacgcc
     9421 agaaagaatc ctccttcgaa caggaaatgg ccgggaaagt ccagcagagg gagcagaagg
     9481 aaaaggaaat cacccccgaa ctcctgagca gataccggat gctcgccgaa aaacgccagg
     9541 gaattgccgt agcccgagtg gtccaagggg tatgccaagc ctgccatatg aacctccgtc
     9601 cccagctcta catcgaactg cagaagcagg actcactgat catttgtccc aactgcagcc
     9661 ggattctctt ctgggacaat ggcgcgccca aagctgaaaa gcaatgaggg aagacccccc
     9721 tcctgattcg ccccgcctct tcgaggaaaa aaaggagact tccccttctt cggaaggaag
     9781 aatcctccgg cacctctctc aaaccctgag tgtcaccaaa acactcaagc gctttccttc
     9841 tctcaccccc caatatcttc aagaggtctt gcagcgctgt gccgaccggg ccgaagaaga
     9901 ccgcaaggct cctcccccag accttcagac gggggaattc cccgaattct tcatccatgc
     9961 cgatggggcc tcgcggggga atccagggga ggccggggcg ggagcggtca tctccgattc
    10021 ccagggccgc actctcaaag aattgaaggc ctatctgggg atagcctcca ataatggggc
    10081 cgaatatcag gcggcgattc tagccctgga aagggctctt gaactggaag tcaagagggt
    10141 gactctcttt ctcgactcag aactggtcgt ccgccagctt cggggcgagt accgggtccg
    10201 cgagccccac ctcaagtccc tccaccagaa ggcccgggag accttgaatc ggttctccca
    10261 atatagtatt ctgagtatac cgagggaaga aaaccgtcgg gctgaccaac tggccaatga
    10321 agccatagat caaaagataa aagaataggg ggaaagggga agaggggaaa aggcgaaacg
    10381 ataatgaaca aaacactccc tttctcctct tctccatatc ccccctttta tgaccattgc
    10441 ggaagggggt cgggggatcg cccgccaggc aaagggcttg gtgggaggaa agtccgagct
    10501 ccgcagggca gggtggtcgc taacggcgac caggggtgac cccagggaaa gtgccacaga
    10561 aaacataccg ccccgagctt gtcgaggggt aagggtgaaa aggcgaggta agagctcacc
    10621 gcggccctgg tgacaggact ggcacggcaa accccacctg gtgcaagacc gaatagggga
    10681 gtgtttgaga gcggcccgct cgatgctccc gggtaggtcg ctggacccag gcagcaatgc
    10741 ctgggctaga ggaatgatcc ccctccccgg gtcttccggg gagacagaac tcggcttata
    10801 gaccccgctt ccgcttttct aattacaagg ggaaaaggcg aagaggcgaa agggcaaaaa
    10861 gggttgaagg ctcgtgaaac tttttttcgc ctcttccccc tttcgccttt tctcctcatc
    10921 tccttgcatc caagagacaa cctttcatgc caactaaaat ttttcagaaa aggtagctga
    10981 gatgcccatg tacgaattcg aagggaaacg acccagggtc ggtgaaacct cttttgtcca
    11041 tccctgcgcg gtgctgatcg gaggcgtgac cattggggaa aactgctaca tcggcccggg
    11101 agcggttctg cggggagatt tcggctatat cgaaatcggc gacggctcca atgtccagga
    11161 gaatgtggtc atccacactt ttcccgagca gactgcccgg ttggcaaaag acagccacat
    11221 cggtcacagc gcaatcgtgc acggctccaa gatcggggaa aatgtgttaa ttgggatggg
    11281 agccatcctt cacgaagggg tggagattgg aaaggattgc atcataggtt ccggctgcgt
    11341 gatcaccgat aaccggaaaa tccccccggg aaaactcatg gtcggagttc ccggcaaaat
    11401 tattgccgac gtctccccgg aaatgacccg ggctaaagtc gccggtaccc gcctctacca
    11461 aacccttccc cgccgttacc gggagaccat gagattgatc gaaagttagt cttatcgtcg
    11521 cttagccgat tgatcattga gtctttcacc tattttgact atcggactgc gcggcggata
    11581 gacggttgga cttcttaaat tgttccttgt tcctgcggac caggcgaaag agtggctggc
    11641 caaggggatg cgtctcttca ctttccagaa cgacgtccgc atgctcatgg atgcggggaa
    11701 agccaatacg gctcagttga gggaatttat cggcaaaaaa ggtgagggtt tttccgggac
    11761 agaatcgtag gggcggttcg cgcgaacgat ccctactcca aaaacatctc ctgggtggcc
    11821 tcttcaccgg gtctcaatac cacaaaatcc ttatcccacg gcccccggat gattttgcga
    11881 aaaaaatcca gcgagccttt gatctcctca tatttcaagt tcaaaaaccg ggcattttcc
    11941 cgggcgtgtt gccgatgggt ctcctccatg ccgagctccg actcaacgag ggcgatgcgg
    12001 gtatagttct tcagctcttc ccggattacc cactctgcgg tttctttgcc gtagcgctgg
    12061 cagtactctt caaaaattcc cagaggagat ttcttttctt cgatccaccc tttagtcagg
    12121 tagtaggtgc cgggctcccg ctgatgttct tccatgtatc gggcattcga gcccaggaat
    12181 agagaaatgc agtcatgcac tttggggatc aggagcggat ggcggttcgc ccggaccccc
    12241 aaaattccgt tgctgcacag tccgtaagcc aggatgatct gatcgccgtc ccatgtctcc
    12301 gttttatcga tctcctcctg gatggctcct ttcattttct gcggagtccg atgcagagac
    12361 tgttccagga atatgaagga ggcctcgtcc gattggacat ggagaagctc ttctttggag
    12421 atgttacagg cgataaccag ggatttcttt ttcttcatgg tttgcc
//
LOCUS       DASPRR010000124         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_835557, whole genome shotgun sequence.
ACCESSION   DASPRR010000124 DASPRR010000000
VERSION     DASPRR010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835557"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="hypB"
                     /locus_tag="VEL68_04550"
     CDS             complement(<1..587)
                     /gene="hypB"
                     /locus_tag="VEL68_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HXZ35271.1"
                     /translation="MKISVVKNILEANQRIAEENRKRFSEEELLVINLMSSPGAGKTS
                     LLEKTIHALKDEYGVGVVEGDIQSTYDAERISRAGAPAVQINTGGACHLDSNMVQEAL
                     KGLELDKLSLLFIENVGNLVCPAEFHLGEDFKAMILSVAEGDDKPLKYPLMFHESRVL
                     LINKIDLLPFCECNPDLIEERAKKINPSLTIFRVS"
     gene            complement(718..1578)
                     /locus_tag="VEL68_04555"
     CDS             complement(718..1578)
                     /locus_tag="VEL68_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HXZ35272.1"
                     /translation="MENLADKHLQRLRQARGRKPNYREVLDLAERMLIEKCRIKDQPF
                     LSSFTLDPVKARPRMEKGLPYLGPERQGLPPAPIEEIFSCLLESFRELNPGRHESMQR
                     AMKARDWALDRDLGRLLANQLSEANLETELGAEGSLLFFFLVQSLKPVLENLAEQWRA
                     ALKGFSWDKGFCPFCGGSAGMGEIREEGRRFLHCPLCGTEWEYPRMKCPYCQNEDQEQ
                     HTYFQVEKEIENRVDICLACRHYLKTVDSREMDGPLDFEVEDYLTLHLDHLAQEEGYQ
                     RPEKLFVEIQ"
     gene            complement(1783..2436)
                     /locus_tag="VEL68_04560"
     CDS             complement(1783..2436)
                     /locus_tag="VEL68_04560"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXZ35273.1"
                     /translation="MAKPKNLIQASTAFERVVHWVLGGSCLFLFLTGFGLMFHSLSFI
                     SRLFPPIPTLAGQGYESMKLLHNLCGLLFAVSFLPALLIWGKDTLFLNKADIEWFKVL
                     GGYVWEVEQVPEAGKFNAGQKVFFIVLLLVWLMMVGTGIFMWFPVLGPFLTRWLYVLH
                     VLGVLTLGSFLVIHFYLGTYGNPGTFQVMGHGFVTKAWCKKHRPKWLKDVEAGRRSI"
     gene            complement(2429..>3001)
                     /locus_tag="VEL68_04565"
     CDS             complement(2429..>3001)
                     /locus_tag="VEL68_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ35274.1"
                     /translation="RDACLHCTDPGCQRACPVPGCITKTPEGAVFINQELCIGCKMCV
                     NACPFEVPRINAETNRAYKCTLCRDRISRDLIPACAKACAMGTLTFGNKDVMIKRAYA
                     RAKVLGEKATVYGDKYLDGTHVIYVLPENIRMYEKLVINPSIPLSVILWKDVLKPLSV
                     LAIGATLVGTFFHYILKGPKRPEEGGNEHG"
BASE COUNT          650 a          849 c          815 g          687 t
ORIGIN      
        1 cacgagaccc ggaaaatcgt caagcttgga ttgatttttt ttgctcgttc ctcgatcagg
       61 tccgggttgc attcgcagaa ggggaggagg tcgatcttgt tgatcagaag gaccctggac
      121 tcgtggaaca tgagggggta cttcaaaggt ttatcatccc cttcggcaac gctcaggatc
      181 atggctttga agtcctcccc caggtggaat tcggccgggc agacgagatt tcccacattt
      241 tcgatgaaga gcaggctcag tttgtctaat tccaggcctt tcagggcttc ctggaccatg
      301 ttgctgtcca gatggcaggc ccctcccgtg ttgatctgaa cggccggtgc accggcccgg
      361 ctgatacgtt cggcgtcata ggtggactga atgtccccct cgacgactcc caccccatat
      421 tcgtccttca aagcatggat ggttttttcc aggagggagg ttttgccggc ccccggggaa
      481 ctcataaggt tgatgaccag caattcttct tcggagaagc gctttcggtt ttcctcagcg
      541 atccgttgat tcgcctcgag aatgtttttt accaccgata ttttcacggt cggctcccca
      601 aagtaggggc gggtttgaaa cccgcgcgtt cgaagtgaaa acctctgaat aaactcatca
      661 cgcctgatag cccccttatt tgttccgaac tccgaactcc attcctccgg acttttctta
      721 ctgtatttcc acgaacagct tctccggccg ctggtatcct tcttcctggg ccagatgatc
      781 gagatgcagg gtcagatagt cttccacctc gaagtccagc ggcccatcca tttcccggga
      841 atcaacggtt ttcagatagt ggcggcaggc caggcagatg tccacccggt tttcgatctc
      901 tttttccacc tgaaaatagg tgtgctgctc ctgatcttcg ttctggcaat aggggcattt
      961 catgcggggg tactcccatt cggtgccgca cagggggcag tgaagaaacc tccttccttc
     1021 ctcgcggatc tcccccatcc cggcactccc cccgcagaag ggacagaaac ctttatccca
     1081 ggagaatccc ttcaaggccg cccgccattg ttcagccaga ttttccaata ccggcttcag
     1141 ggactggacc aggaagaaaa aaaggagact cccttccgcc cccagctcgg tttccagatt
     1201 cgcttcggaa agctgattgg ccagcaagcg gcccaaatcc cggtccagag cccagtccct
     1261 ggccttcatc gccctttgca tagactcgtg ccggcccggg ttcagttccc gaaagctttc
     1321 caagagacaa gaaaagattt cctcgatggg ggcggggggt aaaccctgtc tttccgggcc
     1381 caggtaggga agtccttttt ccattcgggg tcgggctttg accgggtcca gggtgaagga
     1441 agaaaggaag ggctggtcct tgatccggca tttttcgatc agcattcgct cggccaggtc
     1501 cagaacttcc cggtaattcg gtttccgccc ccgggcctga cgaagccttt gaagatgctt
     1561 gtccgccaag ttttccattt tcgtgaaggt tgtcttattt taggccaccg ttttcgttct
     1621 gtccaggagg aatccggact gcggatttcg ggttgccgaa tgaggatttg caatgaattc
     1681 cttcatccga aacaaaaagg cttaccctga tttaaagagt aagcctttct gaagccagtg
     1741 aattgagctc agaggcgatt ggccgatcgc ccctacagat catcagatcg agcgccttcc
     1801 ggcttccaca tccttcagcc acttggggcg atgcttcttg caccaggctt tggttacaaa
     1861 accgtggccc atgacctgga aggttccggg gtttccgtag gtccccaggt aaaaatgaat
     1921 gacgaggaag gaacccagag tcagcacccc caggacatgg agcacataga gccatcgagt
     1981 caggaacggg ccgagcaccg ggaaccacat gaagattccc gttcctacca tcatgagcca
     2041 gaccaggagg aggacgatga aaaaaacctt ctggccggcg ttgaatttcc ccgcctcggg
     2101 gacctgctcc acctcccaca cgtatccccc caggaccttg aaccattcga tatccgcttt
     2161 gttgaggaac agagtgtctt tcccccagat cagcagggcg ggcaggaagg atacggcaaa
     2221 gagaaggccg cagagattgt ggaggagctt catgctttca tacccctggc cggccagagt
     2281 aggaatgggg gggaataagc gggaaatgaa ggagagggaa tggaacatca gcccaaagcc
     2341 tgtgaggaag aggaaaagac agctccctcc caggacccag tggaccaccc gctcaaaagc
     2401 ggtcgaggct tgaatcaggt tcttcggttt agccatgttc gttccctcct tcttcaggcc
     2461 gcttgggccc cttgaggatg tagtggaaaa aggtccctac cagagtggcc ccgatggcca
     2521 agactgacag aggttttagt acatccttcc acaagatcac cgacaggggg atcgacggat
     2581 tgatcaccag cttttcatac atgcggatat tctccggcag aacgtagatg acgtgagttc
     2641 catccaggta tttgtccccg tatacggtcg ctttctcccc caggactttg gcccgggcat
     2701 aggcccgctt gatcatcaca tctttgttgc caaaggtcag cgttcccatg gcgcaagcct
     2761 tggcgcaggc gggaatcagg tcccgggaaa tgcggtcccg gcagagggta cacttgtaag
     2821 cccggttagt ctccgcgttg atccgaggga cttcgaaggg acaggcgttg acacacatct
     2881 tgcacccgat gcacaactcc tggttaatga acacggctcc ttcgggggtc ttggtgatgc
     2941 aacccggaac cgggcaagcc cgctggcacc cggggtccgt gcagtgcagg caggcatccc
     3001 g
//
LOCUS       DASPRR010000125        11911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2128753, whole genome shotgun sequence.
ACCESSION   DASPRR010000125 DASPRR010000000
VERSION     DASPRR010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128753"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..77)
                     /locus_tag="VEL68_04570"
     tRNA            complement(1..77)
                     /locus_tag="VEL68_04570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Thr,seq:ggt)
     gene            complement(79..155)
                     /locus_tag="VEL68_04575"
     tRNA            complement(79..155)
                     /locus_tag="VEL68_04575"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Gly,seq:tcc)
     gene            complement(212..297)
                     /locus_tag="VEL68_04580"
     tRNA            complement(212..297)
                     /locus_tag="VEL68_04580"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Tyr,seq:gta)
     gene            complement(372..447)
                     /locus_tag="VEL68_04585"
     tRNA            complement(372..447)
                     /locus_tag="VEL68_04585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Thr,seq:tgt)
     gene            complement(496..852)
                     /gene="cutA"
                     /locus_tag="VEL68_04590"
     CDS             complement(496..852)
                     /gene="cutA"
                     /locus_tag="VEL68_04590"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HXZ35275.1"
                     /translation="MSVYLVLSTASSLKEGEKIAQHLIRKGLAACVNVVPRLSSYFLW
                     EGRLCREREVLLLVKTVKGKLRKIVKNIKGLHSYSVPEILFFKAEGGERQYVSWVGKR
                     AGKKNKKNIDNEALKS"
     gene            complement(872..1372)
                     /locus_tag="VEL68_04595"
     CDS             complement(872..1372)
                     /locus_tag="VEL68_04595"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HXZ35276.1"
                     /translation="MRSRKRGRQESLVPLGRVLRPYIHRSGLALKIRENRLIEAWEQI
                     VGKGVAEATEPARLQNRTLQVKVANSVWMQQLQFHKKLMIQKVNEFLGEPFLQDLRFI
                     LGEKAKAEPNRKKRESIPMRELKREEKERIEREVGCLSDGEMREVLSRLFAKGLAVQE
                     GREKKK"
     gene            complement(1594..1884)
                     /locus_tag="VEL68_04600"
     CDS             complement(1594..1884)
                     /locus_tag="VEL68_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HXZ35277.1"
                     /translation="MHNYKGEKILNIWVQPRASRNEILGYQNDLLRLRVTAPPVDGKA
                     NQLVRKILAEALGIPPSRVEIIKGERGRRKKVRILGAPPDQLAKLGNQPDSE"
     gene            complement(1891..2370)
                     /locus_tag="VEL68_04605"
     CDS             complement(1891..2370)
                     /locus_tag="VEL68_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HXZ35278.1"
                     /translation="MKITPLDIQQQQFRVKFRGFDMVEVDNFLDLVANEWEELLRENN
                     QLKEEDHQKAERIEELKGAERELRNALVSAQQICEEIKNNARKEGDLIIEEAKGNARK
                     IIEAAQAQAIQLQTDISQLHRQREEFKASLKSTVDMHLRLLESYREGLSSPAPEKGE"
     gene            3116..4018
                     /gene="proC"
                     /locus_tag="VEL68_04610"
     CDS             3116..4018
                     /gene="proC"
                     /locus_tag="VEL68_04610"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXZ35279.1"
                     /translation="MRKPLGIIGGGNMGEALIAGVLQSGLLSPEEIQFFEPRIDRRDF
                     LRDKYRVPSAKSNEDLVSYCPIIILAVKPQSVPEVLPEISRCLKRDHLLISICAGVPL
                     DYLQSFCAVPVRMVRAMPNTPALILKGATALAPSPKAQAEDLSTAEAIFQAVGITAIV
                     KESLMDAVTGLSGSGPAFVFAVIEALAAGGVKEGLSQEAALALTTQTVLGAAHLIQST
                     GKHPATLRDQVCTPGGTTIAGLYAMEEGGFRLALMNAVIAATRRSKELGEALQPPSWP
                     SPGRAEEKTMRKGKQPPKGKRKPR"
     gene            complement(4519..4746)
                     /locus_tag="VEL68_04615"
     CDS             complement(4519..4746)
                     /locus_tag="VEL68_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35280.1"
                     /translation="MKPEIAETAWVKEMKKKVEVEMARREMETIHYWKGEVEKILGKR
                     PESLAAFQIEVRNLLQRMQNRIGVLKSSLAG"
     gene            5212..6297
                     /locus_tag="VEL68_04620"
     CDS             5212..6297
                     /locus_tag="VEL68_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXZ35281.1"
                     /translation="MSDLTSPEASGGSPSFDRIADGKFIENRQSYQFHYTVDPILQAS
                     IEEVLRAHKPPYGVFVALNPITGKILSLAEYSRGPSGNGGIWNRATYPAASVFKIITA
                     ACALEKGALNYNSSVSFGGNQYRLGPSKISTTSKWEHQTQFDEALGKSNNVVFGRVTS
                     HLVGSQTLRQYSSAFGFNQPLPFDFPVDMSKAVIPEESYELARCGAGFGEVTLNPLHA
                     ALIAAAVANQGVMMRPYLIETVRNSEGEILYQAQSKVWTQPISARTAMELNLMMRRTI
                     DDGTASRTFQRYGKDLLKKVTISGKTGSLSGDNPPGLYDWFVGFAPAEDPQIAFSAMV
                     INYQRYKLRGAFVAQEALRIFFRDRSN"
     gene            complement(6302..6568)
                     /locus_tag="VEL68_04625"
     CDS             complement(6302..6568)
                     /locus_tag="VEL68_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35282.1"
                     /translation="MKCPHCGQRIGGRNCPSCSAEVLEESLFCHRCGLKLGKEESLPS
                     PSEKKEEDIDFSRRILCSDGNCIGVVNEQGFCKVCGKPYTGDPT"
     gene            6723..7565
                     /locus_tag="VEL68_04630"
     CDS             6723..7565
                     /locus_tag="VEL68_04630"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ35283.1"
                     /translation="MIIDAHTHLFPEEVRKRRRAYGSRDEAFSLLYGNEKARLAGLED
                     LLEVMDREGVDQSVICGFPWSDPGLCREGNDYLLSCFQRCPSRVLPFVCFPPRAARQA
                     EKELHRCLSLGAKGIGELAFYGGRISAQDIDRLSSLLKPLGGTGIPLLLHVNEPVGHE
                     YPGKSLKTLQPLYQLLLQIPEVQVILAHWGGGFFFYELMPEVAQAAQKVYYDTAASPF
                     LYRPQVYSLAVQIVGPSRILWGSDFPLISPGRYFRELDAAGISSPVRGQILGENARQL
                     LGLR"
     gene            7634..9391
                     /locus_tag="VEL68_04635"
     CDS             7634..9391
                     /locus_tag="VEL68_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXZ35284.1"
                     /translation="MIKKMLINAQDPEESRMAIVEDGILAELIVETSLAAPTRGNIYK
                     AKIVNVEPSLQAVFVDYGEPRHGFLPFSEIHPSYYAPSTPEREKESRLRIQELIRRNQ
                     EVLVQMERGEMGTKGAALTTYISLPGRYLVLMPGSDGGGISRKIEGEKERRKIKEIVQ
                     ELEVPAGMGVIVRTAGLDRAKTDLGKDLQYMLRLWESIQGKAEKTSAPALIYKERDLV
                     IRSIRDYFTPEVEEVLIDNKEIFNQAKEFFRTIMPRYQNKLKLYGEKRPLFSKYELEK
                     QIETIYQRRVPLQSGGSISVDPTEALVSVDVNSGRATQGRGMEETALRTNLEAAGEIA
                     RQLRLRDLGGLVVIDFIDMYDRKHKQEVERSLRNALKRDKARMEVGRISRFGLLELSR
                     QRIRSALSEKTFTPCENCEGTGLVKSTESAALSVLRQVRAGLARGGCTQVRVELPDEV
                     ATYLLNQKREELFRLEKLFGLKIQIAGHPGLPHHRTHISFHRGECSIRPEKTHRETKE
                     ALLAPDARRTRVTSEEADRLAIEELKKETKESFLKKFFWPPSLWREIWKPSSPPPSSL
                     SKGHGAVRETSEAPPKSET"
     gene            complement(9568..10380)
                     /locus_tag="VEL68_04640"
     CDS             complement(9568..10380)
                     /locus_tag="VEL68_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ35285.1"
                     /translation="MLEDRVAIVTGSGRGIGRAVALLFAQEGAKVVVSDIDPAPAQET
                     ADEIKKSGGESLAYPGDVTKPEFAEGIVKKAVEAWGGLHILVNNAGFTWDAVIHKMTD
                     QQWEAMLGVHLTAPFRLIRAAAPYFREAAKQEKGAEKVVNRKIINVSSIAGTRGNAGQ
                     ANYAAAKSGTIGLARALAKEWGPLNVQVNAVAYGWIDTRLTKEKEAGKTLDRDGQSVA
                     IGIPAGLRNMMRMVIPMGRAGTPQEAAGPILFLACPLSDYVSGQCIEISGGF"
     gene            complement(10487..11686)
                     /locus_tag="VEL68_04645"
     CDS             complement(10487..11686)
                     /locus_tag="VEL68_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXZ35286.1"
                     /translation="MATGIRDKVAILGMGCTRFGERWESSGEDLIIEAFQEALADAGI
                     EKREIQAAWFGTHIDEVNVGKSGVPLATTLKLPFIPVTRTENYCATGTEAFRGACYAV
                     AAGVYDICLALGVEKLKDTGYGGLPATTAFGQMQALVGPNATAPGLFAQLATSYFAKY
                     QVDPKLGKRAIAHISAKSHANGALNPKAHLRKAVAEEQIINAPMIAYPLGLFDCCGVS
                     DGSAAAIVTTPEIARSLKRTNPVLVKALAISVSSGEEAMFTNWDGAHLETTFRAGQMA
                     FKEAGIKDPREEVSMMEVHDCFSITELTVYEDLQISPRGKAIEDILEGFFDLKGKVPC
                     QPDGGLKCFGHPIGASGLRMLYEMYSQLLERWPEERRVKNPRFGLTHNLGGIPSTNVC
                     SIAIIGR"
     gene            complement(11687..>11911)
                     /locus_tag="VEL68_04650"
     CDS             complement(11687..>11911)
                     /locus_tag="VEL68_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXZ35287.1"
                     /translation="YTGDMLAASYDPPELYGYVQFDGGGRILCNFTDCTLEDLKVGMP
                     VAFTFRRKYYDEKRDIHGYFWKAVPQKEVV"
BASE COUNT         2585 a         3477 c         3032 g         2817 t
ORIGIN      
        1 tggagcccac gaccgggatt gaaccggtga acctcatcct taccaaggat gcgctctacc
       61 aactgagcta cgtgggcttg gagcgggaaa cgggattcga acccgcgacc cacagcttgg
      121 aaggctgttg ctctagccag ctgagctact cccgcccgga gattccttca aaaaagtcag
      181 gatcagccgc tgaaaaccgc cggacccggg gtggtggaga ggggaggatt cgaacctccg
      241 aaggctaaag ccgacagatt tacagtctgt tccctttgac cgctcgggaa cctctcctca
      301 agcagttatc agctttcaac tgccagctct tgtgttttcg agcgctgcca gatgaaagat
      361 gagaactttg ctggagctgg cgatgggact cgaacccgca accggctgat tacaaatcag
      421 ctgctctacc gattgagcta cgccagcaaa aaggggggta atcccctaag aaaattaggc
      481 attaaataaa atatgttaac tttttaaagc ttcattgtca atattttttt tattcttttt
      541 cccggccctt tttcccaccc aggacacgta ttgcctctcc cccccttccg ccttaaaaaa
      601 caaaatctcc ggaacagaat aactgtgtaa gcctttaata tttttcacta ttttcctcag
      661 cttccctttg accgttttga ccaataacag aacttccctt tcccggcaca gccttccttc
      721 ccacaaaaaa tacgaagaga gcctcggaac cacattaaca catgcggcca gcccttttcg
      781 aatcaaatgt tgggcgatct tttccccctc tttcagcgaa ctggccgtag aaagaaccaa
      841 ataaacgctc accgaaataa cctccttccc gtcatttttt cttctcccgg ccctcttgga
      901 cagccagccc tttggcaaaa agccgggaaa ggacttctct catttcccca tcgctcaggc
      961 acccgacctc tcgttcgatc ctttccttct cctctctttt caattcccgc atggggatgg
     1021 actccctttt cttccgattc ggttctgctt tagccttctc tcccaggata aaacgcaggt
     1081 cctgcagaaa aggttctccc agaaactcat tcaccttttg gatcatgagc ttcttatgga
     1141 attgaagctg ttgcatccag acggaattgg caactttgac ctgtagggtc cgattctgca
     1201 ggcgggcggg ttcagtggct tcggccaccc cctttcccac gatttgttcc caggcttcga
     1261 tgagcctgtt ctcccggatt tttaaagcca ggccggaccg atgaatgtag ggacgaagga
     1321 ccctccccag agggaccagg gattcctgcc tacctctctt cctggatctc attatctaaa
     1381 tctcggccat tttttggcag atgggcgtgc gcaattcggc gggagcccga atctcctcaa
     1441 gaggctccca tgaattccca gaagaatctt tttggggaag gacctacatg ggcagagtcg
     1501 catcccaccc ctctattggg gggaatgggt cctttcggtc cgcgggaaca gtttccctcg
     1561 acgaaagcag aggccccctc agccctgcgg gcatcactcc gaatcgggct gattgcccag
     1621 cttggccagt tgatcagggg gcgccccaag gatacggact tttttccttc gccccctttc
     1681 tcccttgatg atttccaccc ttgaaggagg gattcccagg gcttcggcaa gaatttttct
     1741 taccaattga ttggctttcc catcgacggg gggggcggta actcgaaggc gcagaagatc
     1801 gttttggtac cccaggattt cattccggga ggcccggggt tgaacccaga tattcagaat
     1861 tttttcgcct ttatagttgt gaattttctt ttattcccct ttctccgggg caggggaaga
     1921 gaggccttct cgatacgatt caaggagccg aagatgcata tctaccgtgg atttcagcga
     1981 cgctttgaat tcctcccgct gacgatggag ctgggagata tcggtttgca gttgtatggc
     2041 ctgagcttga gctgcctcta tgatcttgcg ggcattccct ttagcctcct cgatgatcag
     2101 gtccccttcc ttgcgggcgt tgttttttat ctcttcgcag atttgctggg ccgaaaccag
     2161 ggcatttctc aattccctct ccgccccttt gagttcctcg attctctccg ctttttgatg
     2221 gtcttcttcc ttcagctggt tgttctcccg aagcaattcc tcccattcat tggccacaag
     2281 gtccaaaaaa ttatccactt caaccatgtc aaaaccacga aatttgactc ggaactgctg
     2341 ctgttggatg tccagagggg taatcttcat gggttttctc cttctcttcc ttggccttca
     2401 acagccctcc aggggtaggc ccctctccgc tgcggaacag atgtattatc ttcaacatct
     2461 cagtagaaga aggctatctt cataattttt tcccatatta gaaacattgc cggaaaaagt
     2521 caaggttgct ttgggtcttt gggtcttttc cacccacatt ctggagatgt tcttttgcga
     2581 ggcccggggc ccaaacagaa tatcgagcca tctgaatagg gagcaagggg ggttggcttg
     2641 cccaacccca gttcttaaaa aagagttctt cgaaaataag atctctggaa aggagggggg
     2701 atctcatggg gttcagggcg gagaaatttg gatcatcatc ctgaggcggt ggctcttcca
     2761 agggatcggg cctgtcctgg ggggattgaa acggaaaaat gctttagttc tcttttttgc
     2821 ttggaggtat ctcctttcag gcctctccct gcggaacatg gggaggagtt tgatggaagg
     2881 ataagcaccc gccgagcctg atgatccaaa ttcctccacc ctgaacccca acaagcaaga
     2941 aaaactgggg gtgggtaaga gggggttggg cattgacttt ctcctgctcc cttcagtaga
     3001 ctgcaataga tagtcctgag ttcggacctc agtgaccatt taaaaaactt ttcttccttt
     3061 ctttctttct tattccgggc acaccgaact cgcggcaact accggggagg attctatgcg
     3121 caaacccttg gggatcatcg gagggggaaa catgggggag gctttgatcg caggagttct
     3181 tcaatcgggc cttctttccc ctgaagaaat ccaattcttc gagccacgaa ttgatcggag
     3241 ggattttcta cgggacaaat accgggttcc ttcggccaaa agtaatgaag acctcgtatc
     3301 ctattgcccc atcatcatcc tggcggtaaa accacagtcc gtgccggaag tgcttcccga
     3361 aatctcccgt tgcctaaaac gggatcattt gctcatatcc atttgtgcgg gcgttccatt
     3421 ggactacctt cagtcttttt gcgccgtccc ggtgcgcatg gtcagggcca tgcccaacac
     3481 cccagccttg atcctaaagg gagctaccgc acttgcccct tccccaaagg cccaggcaga
     3541 ggacctttcc accgccgagg ccatttttca ggctgtgggg atcaccgcca tcgtcaaaga
     3601 atccttgatg gatgcagtga ccggcctgag tgggagcggc ccggcctttg tctttgcggt
     3661 gatcgaggcc ttggccgcgg gaggggtaaa agaaggacta tcccaggaag cggccttggc
     3721 cctcaccacc cagaccgtct tgggagcagc gcacttgatt caatccacgg ggaagcatcc
     3781 cgccaccctc cgcgaccagg tctgtactcc cggcggaacc accattgctg gtctttatgc
     3841 catggaggaa ggcggtttta ggctggcttt aatgaatgcg gtcattgccg ctacccggcg
     3901 ctctaaggaa ttgggagagg ctctccagcc cccctcttgg ccctccccgg gccgcgccga
     3961 ggaaaagact atgcgaaagg gaaaacaacc cccgaaaggg aagcgaaaac cccgctgacg
     4021 gagcgccttc ccgggatgaa ggactgcttc cagaactcaa aggagtacgg tcaagcccct
     4081 taccaccggc ccatgtcccc agcactccgc agagaccaaa ctcggttaca cccccggtaa
     4141 aaccgaggtt ttacccaaag gaaatgataa tcctttgaaa tctccctgtc tctacagcca
     4201 atcatgtgtc aaaaatttgt cttaaataat gctttgaaac cagttctccc ccttatcgtc
     4261 tctcgttgga aaaactattt agcaaagaaa tcaaattgcc ataataagca aagatgaatt
     4321 tccatttatt tattctgtat tcccccatcg aaaaaaactg ctcccaattt ttcgtcgtga
     4381 atgaatcctt ggcatgtttt ttgaaaataa attccttgtc caataaaaaa gggaatagga
     4441 aaattgacct tgtcgccgac aaagccccga aaaaatttga aatatttatc tctcgccgaa
     4501 ggggctatgg actttttcct agcccgcaag ggaacttttg agcaccccga tacggttctg
     4561 cattcgctga aggagatttc gaacttcgat ctggaatgca gccaggcttt cgggacgctt
     4621 gcccagaatt ttttccactt cccccttcca gtaatgaatg gtttccattt ctcttcgggc
     4681 catttccact tcaacttttt ttttcatctc tttgacccac gccgtttctg cgatctcggg
     4741 tttcatgaaa gactcctcct tttttccttc ctcagcggtc cggaaaccac ccaaccaagg
     4801 aaggaagaaa tcttccccaa tgtatccccc gaaataaccg tccagcaagg agaaaattcg
     4861 gggtggggga aaagccatcc ctcctcccct tccctccccc cattcttttt tgagttgaca
     4921 ggtgctgatc aagggcatag gattcataag gaatcttttg ccggaggttc tcatgagacg
     4981 gacgaccctg atcctttccc aggatttgag agaatgaaat tcggaacgag gaggaaaaag
     5041 ggaaacccgc cagaaaaaac tcccccaacc ccttggacag cccccctccc acggcgatcc
     5101 tggaggaagc cgtttctctg ggtcttcctt tgcctggcgg tattcttttg ggccagggct
     5161 ccttttcttc atgcggcccc caaggccacc cgcatctcga ctccttctcc tatgtccgat
     5221 ttaacctccc ctgaggccag cggaggttct ccttcttttg atcgaattgc ggacggaaaa
     5281 ttcatcgaaa atcggcaatc ttaccagttc cattataccg ttgatcctat ccttcaggca
     5341 tcgatcgagg aagtcctccg agcccacaag cctccatacg gcgttttcgt ggctctgaac
     5401 cccataaccg gaaagatcct gtccctagct gagtattctc ggggtccttc cgggaacggg
     5461 ggcatttgga accgggccac ctatccggcg gcttcggttt tcaagatcat caccgccgcc
     5521 tgcgccctgg agaagggagc gttgaattac aactcttccg tttccttcgg gggaaaccag
     5581 taccgcctgg ggcccagcaa gatcagcacg acatccaaat gggagcatca gactcaattc
     5641 gatgaagcct tgggaaaatc gaacaacgtt gtttttggcc gagtgacctc ccatctggtg
     5701 ggttcccaga ccctccgcca gtattcttcc gccttcgggt ttaaccagcc ccttcctttc
     5761 gactttcccg tagacatgag taaagcggta atccccgagg agagctatga gttggcccgt
     5821 tgcggagcgg ggtttggcga agtcaccttg aacccccttc acgccgcgct catcgccgcg
     5881 gcagtcgcca accagggagt catgatgcgg ccttacttga ttgaaacggt ccgcaactcg
     5941 gagggcgaga ttctctacca ggcccagtcc aaggtctgga cccagcccat ttcggccaga
     6001 accgccatgg aactgaacct tatgatgcgc cgcacgatag atgatggaac cgcttcccgc
     6061 acctttcaac gctacggaaa ggacctcctg aagaaagtga caatcagcgg gaaaaccggg
     6121 tctttgagcg gggacaatcc ccccggtctc tacgactggt tcgtgggatt cgcccctgcc
     6181 gaagaccccc agatcgcctt ctcggccatg gtcatcaact accagcgcta taaactccgg
     6241 ggggcctttg tcgcccagga ggctctgagg atttttttcc gagatcggtc gaattgagga
     6301 ttcaggtagg atcgccggta tagggtttcc cacatacctt gcagaaaccc tgctcattta
     6361 ccaccccgat gcagttccca tcgctgcaca ggatgcgccg ggagaagtca atgtcctctt
     6421 cttttttttc tgatgggctc gggagggatt cctccttccc cagtttcaag ccgcagcggt
     6481 ggcagaaaag gctttcttcg aggacctccg cgctgcagga tggacagttc cgcccgccta
     6541 tcctctggcc gcaatggggg catttcatgt ttccaggctc cttgggtagg tgagaaatta
     6601 gtgttcaaaa gacatttacc atatccgggc ggattcttca acccttctga gaaaactgcg
     6661 acaagctctt tgacgaaggc cggaaaaatt atcactccgt tctcatgtgc ccattgccat
     6721 ccgtgatcat cgacgctcac acccacctat ttccagaaga ggtccgaaag agacgaagag
     6781 cctatggctc ccgggacgaa gctttctcgc ttctttatgg aaatgaaaaa gcccgccttg
     6841 ccgggctgga agaccttctt gaggtcatgg atcgggaagg ggtggaccag agcgtgatct
     6901 gcgggtttcc ctggagcgac ccgggactct gccgggaagg gaacgattac ctcctttcct
     6961 gcttccaaag atgtccttcg cgggtccttc cctttgtctg cttccctcct cgggcagccc
     7021 gccaagcgga aaaggagctg caccgctgct tatccctggg ggctaaggga atcggtgaac
     7081 tagcttttta cgggggaaga atctccgccc aagacattga ccgcctttct tctctcttga
     7141 aacccctggg aggaaccggg atcccgcttc tcctccatgt caacgaaccg gtggggcatg
     7201 aatacccggg gaaaagcttg aagaccctcc aacctctcta ccagcttctc ctccagatcc
     7261 ccgaggttca agtgattctg gcccattggg gagggggctt tttcttttac gaactcatgc
     7321 cggaagtggc ccaggctgcg cagaaggttt attacgatac agcagcttct ccattcctgt
     7381 accgacctca agtttattct ctcgctgtcc agattgtcgg acccagtcgg attctctggg
     7441 ggagcgactt ccctctgatc tccccgggca gatatttccg ggaattggac gcggcgggga
     7501 tttcttcccc ggtccggggt caaatcctgg gagagaacgc ccggcaactt ttgggcctaa
     7561 gataaaggct ggccttgggg atcaatatca ggtaaaataa ttgccagctt ccatctccct
     7621 tcttcaggat cgtatgatta aaaaaatgct gatcaatgcc caggacccgg aagagagccg
     7681 gatggccatc gtggaggacg ggattctggc cgagctcatc gtcgagacct cccttgccgc
     7741 cccgacccgt gggaacattt acaaggccaa aatcgtcaat gtcgagccaa gcctgcaggc
     7801 ggtcttcgtg gattacgggg aaccccgcca cggtttcctc cctttttcgg aaatccatcc
     7861 cagctattac gcaccctcga ccccggagcg ggaaaaggaa tcccgcctcc gtatccagga
     7921 actgatccgg cgtaaccagg aagtgctggt acagatggag cggggagaga tgggaaccaa
     7981 aggtgcggcc cttaccacct acatctccct gcccgggcgg tatctggtcc tcatgccggg
     8041 gagcgacggg ggagggatct cgcgcaagat cgaaggggag aaagaacggc ggaagatcaa
     8101 ggaaatcgtc caggagctcg aggttcctgc gggtatggga gtgatcgtcc ggaccgcggg
     8161 cctggaccgg gccaagactg atttgggcaa agaccttcaa tatatgctcc gcctctggga
     8221 atcgattcag gggaaagcgg aaaagacctc cgcccccgcg ttgatttaca aagagcggga
     8281 tttggtcatt cgctccattc gcgattactt cacccctgag gtggaagagg tcttaatcga
     8341 taacaaagaa atctttaacc aggccaaaga atttttccgg accatcatgc cccgctatca
     8401 aaacaagcta aaactctacg gagaaaaacg acctcttttt tctaagtacg agctggaaaa
     8461 gcagatcgag accatttacc agagacgggt tcccctccag tcgggggggt ccatctcggt
     8521 cgaccctacc gaggctctgg tttccgtgga tgtcaactcg ggccgggcca cccagggaag
     8581 gggcatggaa gagactgccc ttcggaccaa cctcgaagct gccggggaaa ttgcccgcca
     8641 gctccgcctt cgcgacctag gtggcctggt ggtgattgat ttcatcgata tgtatgaccg
     8701 caagcacaaa caggaggttg aacgatccct gcgcaacgcc ctgaaacggg acaaagcgcg
     8761 gatggaggtg gggcggatca gccgctttgg ccttctggag ctttcccgcc agcgcatccg
     8821 gtccgccctc agcgaaaaaa cctttacccc ctgcgagaat tgcgaaggca ccggtctggt
     8881 caagtccaca gaatcggcgg ccttatctgt tttgcgccag gtgcgggccg gcctggcccg
     8941 tggaggctgc acgcaggtcc gggtggagct gccggatgag gtagctacct acctcctcaa
     9001 ccagaaaagg gaagagctat tccgcctgga aaaactgttc gggctgaaaa tccaaatcgc
     9061 cggtcatccc ggattgcccc accaccggac gcatatcagc ttccatcgcg gagaatgttc
     9121 aatccggccc gagaagaccc accgggagac gaaagaagcg ctcctcgctc cggacgcccg
     9181 tcggacaagg gtcacatcgg aggaagccga tcgtctggcc attgaggaat tgaaaaaaga
     9241 gaccaaggag agtttcctga aaaaattctt ctggcccccc tccctctggc gggaaatctg
     9301 gaagccttct tctcctccgc catcctccct atccaaaggc catggagcag tccgggagac
     9361 ttctgaggcg cccccaaaat cggagacctg atccgcctct ttaccgggcg cgaattaaaa
     9421 aggggccacg gtttcagcca aggccccttt tgatttccaa accgcttcgc cctatgtgga
     9481 gtcctgcggg tattctgaat tcctcttgct gcccttcacg ccctcctgtt cctaattccg
     9541 cattcagcaa tccaaatgcc gcatttttca gaaccccccg ctgatttcaa tgcactggcc
     9601 ggaaacgtaa tcggacagag ggcaggcgag gaaaagaatc gggcctgcgg cctcttgcgg
     9661 ggttccggcc cttcccatgg gaatgaccat ccgcatcatg ttgcgtaatc ccgcgggaat
     9721 cccgatggcc accgactgtc catcccggtc cagggtcttt cccgcttctt tctctttggt
     9781 gagacgggtg tcgatccagc cataggccac agcattgact tggacgttca agggccccca
     9841 ctctttggcc agggcccgtg ccagcccgat ggttccggac ttagccgcag cgtaattcgc
     9901 ctgcccagca ttccccctcg ttccggcgat ggaggagaca ttaatgatct tccgattcac
     9961 caccttctcg gcccctttct cctgtttggc ggcctcccgg aaataggggg cggcagcccg
    10021 aatcaagcga aagggagcgg ttaggtgaac tcccaacatg gcttcccatt gctggtcggt
    10081 catcttgtga attaccgcgt cccaggtgaa accggcgttg ttcaccaaaa tgtggaggcc
    10141 accccatgct tcgaccgcct tcttcacgat tccctcggca aattcgggtt tggtcacgtc
    10201 cccgggatag gctagagact cgcccccgga ctttttaatc tcatcggccg tttcctgggc
    10261 gggcgcgggg tcgatatcgc tcaccaccac cttggcccct tcctgggcaa acagaagggc
    10321 cacggcccgg ccgatccccc gcccggaccc agtcacaatc gccactcgat cctcgagcat
    10381 cttttccaaa gttggtctcc tttcagggca atctcccagc tttcagcgat cagccaaacc
    10441 tctatttttt ttagcggatg gctgaccgct catcgctggc tctgcgttac cggccgatga
    10501 ttgctatgga gcacacgttg gtcgaaggga ttccgcccag gttgtgggtc aggccgaagc
    10561 gcgggttctt caccctgcgc tcctccggcc acctctccag aagctggctg tacatctcgt
    10621 agagcatgcg aagcccggag gccccgattg ggtggccgaa gcatttcagc cccccgtcgg
    10681 gttggcaggg gacctttcct ttgaggtcga agaatccttc cagaatgtcc tcgatggctt
    10741 ttccccgggg agaaatctgc aggtcttcat ataccgtgag ctcggtgatg gagaagcaat
    10801 cgtggacctc catcatgctg acttcttccc tcgggtcctt gatccccgct tctttgaaag
    10861 ccatctggcc ggcccggaag gtggtctcca agtgggcacc atcccagttt gtgaacatgg
    10921 cctcttctcc tgaagagaca gaaatcgcca gagctttaac cagaactgga ttggttcttt
    10981 tcaacgaccg ggcgatctcc ggggtagtca cgatggctgc cgccgagccg tcgctcaccc
    11041 cgcagcaatc aaaaaggccc agtggataag cgatcatggg agcattgata atctgctctt
    11101 ctgccaccgc cttgcgcagg tgggccttgg ggttcagagc cccgttggca tggctcttgg
    11161 ccgaaatgtg ggcgatggcc ctctttccca atttgggatc gacctggtat ttggcaaagt
    11221 agctcgtggc cagctgggca aagagccccg gggccgtagc attgggcccc accaaggcct
    11281 gcatctggcc gaaggcggtg gtcgccggga gccccccgta tcccgtgtct ttcaactttt
    11341 ccacccccag cgccaggcaa atgtcataaa cccccgcggc cacggcatag caggctccgc
    11401 ggaaggcctc ggtccccgtg gcgcagtaat tctccgtgcg ggtcaccgga atgaagggca
    11461 gcttaagggt ggtagccagg gggaccccgc ttttgcccac atttacctcg tcgatgtggg
    11521 ttccgaacca ggccgcttga atctctcttt tctcgatccc cgcatcggcc agggcttcct
    11581 gaaaggcctc gataatcagg tcctcaccgg aagactccca cctttctccg aagcgggtgc
    11641 aacccatacc cagaatggct actttatcac gaattccggt tgccatttaa accacctcct
    11701 tttggggtac ggctttccag aaatacccat ggatgtcccg tttttcgtcg taatattttc
    11761 tgcggaaggt gaaggcgacc ggcatcccta ccttcaggtc ctccagggta cagtcggtga
    11821 agttgcacag gattctcccg cccccatcga attgcacata gccgtagagc tccggaggat
    11881 cataggaagc ggccagcata tccccggtat a
//
LOCUS       DASPRR010000126         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_188596, whole genome shotgun sequence.
ACCESSION   DASPRR010000126 DASPRR010000000
VERSION     DASPRR010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188596"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VEL68_04655"
     CDS             <1..1273
                     /locus_tag="VEL68_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ35288.1"
                     /translation="IIGRAWNEHFWEEKREPTRFDLDDLTPNNPAMMIRACGHSVWLN
                     SAALAEAGINLDTVDPPGSRIERDPVSGEPTGVLKEMRRHMEKFIPSPTLETRKKAAL
                     AGQEEALRFGVTGVHSCETLREWEALSAIEEEGKLKIRVHHTMPYEGMEEGRSRSLKP
                     WQGGERLWFGQVKLFADGSLGSGTALLHDPYADNPSDRGIAVLTLQALSKRIREAYRD
                     GYSVAIHAIGDKALTNSLKAIDSARKAVPGARRDRVEHVQLFRSSDLPLFRRLGVVAS
                     VQPVHLSTDWPTAEKKWGLSRCRNGYAWKSLLGAKIPLNFGSDTPVEPINPLLGLQAA
                     VTRQDLHGKPPGGWFPEEKITLEECIKAFTARPAWCSRKERYLGTITPGRLADLTVFS
                     EDLFQVPPDQWSSVEVEMTLINGEIVYRKSK"
     gene            complement(1354..2286)
                     /gene="arsS"
                     /locus_tag="VEL68_04660"
     CDS             complement(1354..2286)
                     /gene="arsS"
                     /locus_tag="VEL68_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551585.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HXZ35289.1"
                     /translation="MEPFSTTLARHGLELKRGRTTTLQINLGLRCNQACRHCHLEAGP
                     NRTEMMSWETFEEVAGYARRNSFEVIDITGGAPELHPRIIEIVAGLAPLAKKTLFRCN
                     LTVLAEEKREELIEICQAHRVGIIASFPSLDAVQTEAQRGPGVFPKSISSLKRLNEWG
                     YGRPGSELELNLVSNPVGAFLPAPQAQAERRFQSALDERWGIRFHHLYTFSNVPLGRF
                     RRWLIESGNYGKYMQKLAAGFNPCTVGGLMCRSLVSVDWKGVLFDCDFNLAAGLYLGG
                     QKRHISEMAGPPEPGEVIATSDHCYACTAGSGFT"
     gene            complement(2438..2773)
                     /locus_tag="VEL68_04665"
     CDS             complement(2438..2773)
                     /locus_tag="VEL68_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HXZ35290.1"
                     /translation="MSEPYYDPLDLPKFPEIGEEAPELAKKFFDYYGAVFAEGALSER
                     EKALIALAVAHAVQCPYCIDAYTQACLEKGSNLKEMTEAVHVSVAIRGGASLVHGIQM
                     RNAAKKISM"
BASE COUNT          692 a          856 c          884 g          665 t
ORIGIN      
        1 gatcatcggc cgggcctgga acgagcattt ctgggaggag aaaagagaac ctacccgttt
       61 tgaccttgac gacctaaccc ctaacaaccc agccatgatg attcgagcgt gcggccatag
      121 cgtttggttg aactcggcag cccttgctga ggccgggatt aacctagaca cggtcgatcc
      181 tccggggtct cggattgaaa gagacccggt gagcggagaa cctaccggtg ttctgaagga
      241 aatgcgccgg cacatggaaa aattcattcc ttccccaact ttggaaacac ggaaaaaggc
      301 cgcgctggca ggccaagagg aagccctgcg tttcggggtc acgggggttc attcctgtga
      361 aaccctaagg gagtgggaag ccctatcggc gattgaagag gagggaaagc tcaaaatcag
      421 ggtccaccac acgatgccct atgaaggtat ggaagaggga cggtcccgct ccctaaaacc
      481 ctggcaggga ggggagcgat tgtggttcgg ccaggtcaag ctttttgcgg atggttcctt
      541 ggggtccggg accgctcttc tccatgaccc ctacgccgac aatccctcgg atcgggggat
      601 cgccgttctc actctgcagg cattaagcaa aagaatccga gaggcttacc gggatggata
      661 cagcgtggcc atccatgcca tcggagacaa agcactcacc aactcactga aggcgatcga
      721 ctcggccagg aaagcggttc ccggggcgag gagggatcgc gtcgagcatg tccagctttt
      781 ccggtcctcg gacctccctc tgttccgccg cctgggggta gttgcctcgg tacaaccagt
      841 gcatctctct accgattggc cgacggcgga gaagaagtgg ggactctctc gctgccggaa
      901 cggctacgcc tggaaatcgc ttcttggggc gaaaattccg ctgaatttcg gttctgacac
      961 gccggtcgag cccatcaatc cgctgctggg tctccaggca gcggtaactc ggcaggattt
     1021 gcatgggaaa ccgcccgggg gatggttccc tgaagaaaaa atcaccctgg aagaatgcat
     1081 caaggccttc accgcccgtc ccgcctggtg ctctcggaag gagaggtatc tggggaccat
     1141 aacccctggc cgcttggcgg acctgactgt cttttctgaa gacctgttcc aggtcccacc
     1201 ggatcaatgg tcttcagtag aggtggagat gaccctcata aacggggaga tcgtttatcg
     1261 aaagtcgaaa tagagaggat tctttttgat tcgatggggg gggaagacga tccaattttc
     1321 aacggcccga actcaggccg cgatggcccc gcctcaggtg aaacccgatc cggcggtgca
     1381 ggcgtagcag tggtcggagg tggcgatcac ctcacccggt tccgggggtc cggccatttc
     1441 cgagatgtgc cgcttttggc ctcccaagta caaccccgcg gccagattaa aatcacagtc
     1501 gaataaaacg cccttccaat ccaccgacac gagcgaacgg cacatgagac cgccaacggt
     1561 gcagggatta aagcccgcgg ccagcttctg catgtacttt ccataatttc ctgattcgat
     1621 gagccagcgg cgaaaacgtc ccagggggac gttggaaaaa gtatagagat ggtggaagcg
     1681 gattccccat cgttcatcca aagcgctttg aaacctcctt tcggcctggg cctggggagc
     1741 cggcaagaag gcccccacgg gattggagac cagattcaat tccagctcgg acccgggccg
     1801 tccatatccc cattcattca gccgtttcag gctggaaatg ctttttggaa aaacccccgg
     1861 tcctctctgg gcctccgtct ggactgcatc caaggaggga aaagaggcga tgattcccac
     1921 ccgatgggct tggcaaatct cgatcaattc ctcccgcttt tcttcggcca aaacggtcag
     1981 gttgcagcgg aacagggtct tcttggccaa aggggccaaa ccggcaacga tctcgatgat
     2041 tcgggggtgc aactccggag ccccgccggt aatgtcgatg acctcaaagg aattccgccg
     2101 ggcatagccc gccacttctt cgaaggtttc ccaggacatc atctcggtcc ggttgggtcc
     2161 cgcttccaga tggcagtgcc ggcaggcctg gttgcagcga agacctaagt tgatttgcag
     2221 ggtggtggtc ctcccccgct tcagttcgag tccgtgcctg gcgagagtcg tggaaaaagg
     2281 ctccacgtag cccccccgat cctgcttttc aagatttctg tttaaaaggt tgcgttcaag
     2341 ggccataatt tatcatttat gaggcggatg ccattcagcc gattcagaac ggaaacccga
     2401 aacccctgcc tttctatgga aaacgttgcc agacccatca catggagatt ttcttggcgg
     2461 cattcctcat ttgaattccg tgaaccaggg aggcccctcc gcggatggcc accgaaacat
     2521 ggactgcttc ggtcatttcc tttaggtttg agcccttttc caggcatgcc tgggtgtaag
     2581 catcgatgca gtaggggcat tgaacggcat gggccacagc caaggcgatc agcgcctttt
     2641 ccctttccga gagagctccc tcggcgaaga ctgccccata gtagtcgaag aatttcttgg
     2701 caagttcggg agcctcttca ccgatttccg gaaacttggg aaggtccaag ggatcgtaat
     2761 agggttcgct catagtcctt cctttcgcgg aataaaggat cgatttcttg agggatcaca
     2821 tttattattt catcttttat agagttgtca aagggtaata ccaaatgaat ccgaggaatc
     2881 ggtgtttatc cacgttcatc ggaattccat aaggctttgg agggaggtta ggtgcggctt
     2941 tccgggccgc tctctaccgc tgttccgtac cgtttcttga accacccggg aatcagagag
     3001 acgaggacaa agaaaattgc agcgatggcc aggtacagaa tggttctctt caaagcctcg
     3061 ggctgtcccc cggagaggat cacctcgcgg gccgagc
//
LOCUS       DASPRR010000127         5201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1968754, whole genome shotgun sequence.
ACCESSION   DASPRR010000127 DASPRR010000000
VERSION     DASPRR010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968754"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..894)
                     /gene="thiW"
                     /locus_tag="VEL68_04670"
     CDS             complement(337..894)
                     /gene="thiW"
                     /locus_tag="VEL68_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HXZ35291.1"
                     /translation="MRLWNKSRRRERPIETDIRRVTEIHRVVLTSLFAAVAVLLSGIH
                     FPVGPSKVFPFQHMVNALAGVLVGPWYGALAALIAGIIRNAMGTGTLFAFPGGIPGAA
                     VVGLAYRWTRRDWASLTEPLGTGVIGVGLVVLLLGPLMGKEYAFIFFLTAFLASSIPG
                     SILGFFLLKALRRTKILERRQTPGS"
     gene            complement(851..1681)
                     /gene="larE"
                     /locus_tag="VEL68_04675"
     CDS             complement(851..1681)
                     /gene="larE"
                     /locus_tag="VEL68_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HXZ35292.1"
                     /translation="MSESKAEKARQILREMGSVLVAFSGGVDSTLLLRLAHEELGDRA
                     MALIASSPTYPSSEVDQAKKLAEEMGVRYVEVFSNELKIPSFVQNSPSRCYHCKTELF
                     VLCRERAKALGLKFVSDGSNWDDTGDFRPGMEAARELGVRSPLKEAGLTKEEIRELSK
                     SMGLPTWDKPSLACLASRFPYGTEITPLRLEQVQKSEEFLRALGLGQLRVRYHGNLAR
                     IEVPSGDFKRIMEGGKREQVIRALKEAGFTYVTLDLQGYRTGAMNEALEQKQKKGEAH
                     "
     gene            2084..2545
                     /locus_tag="VEL68_04680"
     CDS             2084..2545
                     /locus_tag="VEL68_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXZ35293.1"
                     /translation="MAESFRSIPYGVYLLTARKGPDLFSVVVSWVSQVSFSPPRLMVA
                     LRHNRRVIPAIQESRFFSLSLLSTDQKALVARFKGLNPPAGRGDLWEEADSFHAPILK
                     GCLAGWQCRLFSTVEAGDHFLFLGEVESASGRKEGLPLTTLDLGKTYIGEF"
     gene            2561..3202
                     /gene="nth"
                     /locus_tag="VEL68_04685"
     CDS             2561..3202
                     /gene="nth"
                     /locus_tag="VEL68_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXZ35294.1"
                     /translation="MKRNNTIVKVLDILQKEYPDARVTLDFKDPLQLLIATILAAQCT
                     DERVNQVTKDLFKKYRKASDYAGADLKTLEEEIRSTGFFRNKAKSIVGCCQMLVQKFG
                     GQVPRTLEELTSLPGVGRKTANILLGNAFNQQAIAVDTHVKRVTHRLGWAKSEDPDKI
                     EFELMEVIPQNRWTLSCHQIVFHGRRVCMAKNPQCPTCPVAKLCPKIGVAQKE"
     gene            3292..3924
                     /locus_tag="VEL68_04690"
     CDS             3292..3924
                     /locus_tag="VEL68_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ35295.1"
                     /translation="MMFLKQFEVSGMAVFAYLLGEEGGGPGLVIDPADDADRILAEAE
                     KNRIKVQYILNTHAHVDHVMGNEEMKRKTGAPILIHEVDAPSLTKIPRAMLAMFGGRP
                     SPAADQTVKDGDVIQVGSLSLKVLHTPGHSPGSVCFHGDGMVFTGDTLFVGGVGRTDL
                     PGGSWPMMLRSIQTKLLILPDETLVLPGHNYGATPTSTIKNERLHNPFLK"
     gene            complement(3997..4140)
                     /locus_tag="VEL68_04695"
     CDS             complement(3997..4140)
                     /locus_tag="VEL68_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35296.1"
                     /translation="MALSCEEYRDQQQLLALKKRLSENKLDPEEREEIERLVRDLEIK
                     LKM"
     gene            complement(4509..>5201)
                     /locus_tag="VEL68_04700"
     CDS             complement(4509..>5201)
                     /locus_tag="VEL68_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ35297.1"
                     /translation="VTPSIYGVDNSATLWGIKARGNDARGVALINDKTTDNELDALGH
                     GGVRGIRLNLKTVRITDPAVAVQMFQAVAERMKKRNWHIQLWATMEMIEAIKAQLAAS
                     PVPLVFDHFAGAEPEKSVDQPGFDNILDLVRTGKAYVKISIPEGPRHDYTGFLPLAKA
                     LIAANPDRILGGTNWPHPNAVTPPGRKPTDVTPLWQVDDGLVLNLLPVWAPDPAIRKK
                     ILVDNPARLYGF"
BASE COUNT         1137 a         1506 c         1399 g         1159 t
ORIGIN      
        1 gaatcgcacc gagtgaaggc atctcctttc aagccttttg aaatcatatg ccttctccat
       61 ttcaaaagta aagtggattt ttcaaacccc gattccttct tccatgtttg cttctgaggg
      121 gagggcaaag tcttaacctc gcctcctgct ctcctcgccc ccgctaatcg caattggcgc
      181 gactttcata agagaagacc ctgccatgaa agcggatggg gacccggttg gaaagcggaa
      241 aagggataaa gaatcggggt cctttagacc agggaaaaag caggtaggaa aatgactcgg
      301 tcgcaccggc tcatcaaggc ttccatttcc gaggttctaa gatccaggtg tttgtcgtcg
      361 ctcaagaatt ttcgtccggc gcagagcctt cagcaggaaa aaacccagga tcgagccggg
      421 aatgctcgag gctaggaagg cagtaaggaa gaagataaag gcatattctt tacccatcag
      481 tggaccgagg aggagaacca ccaagcccac tccaatcacc cctgttccca agggttcggt
      541 gagagaagcc caatcccttc gtgtccaacg gtaggccagt cccacgacag ccgcgcccgg
      601 gattccgccg ggaaaggcga agagggttcc ggttcccatg gcattgcgga tgattccggc
      661 aatcagggcg gctagcgccc cataccacgg ccccaccaat accccggcca gggcattgac
      721 catgtgctgg aaggggaaca ctttgctggg ccccacagga aagtgaatcc ccgaaaggag
      781 caccgccacc gcagcaaaaa ggctggtcag gaccacccga tgaatttcag tcacccgacg
      841 gatgtcggtt tcaatgggcc tctcccttct tctgcttttg ttccagagcc tcattcatgg
      901 ccccggtgcg ataaccctgc aggtcaaggg taacgtaggt gaaccctgcc tccttcaagg
      961 cccggatcac ttgttccctt ttgccccctt ccatgattcg cttaaaatcc ccggagggca
     1021 cttcaattcg ggcgagattc ccgtgatacc gcacccggag ctgccccaat cccaaggcgc
     1081 gcaaaaactc ttccgacttt tgtacctgtt ccagacggag gggggtgatc tccgttccgt
     1141 agggaaaccg ggaagccaag caagccaaag agggtttgtc ccaagtggga agccccatgg
     1201 atttgctcaa ttcacggatc tcttctttgg tcaggcccgc ctctttcagg gggctgcgga
     1261 cccccaattc ccgggctgcc tccattcccg ggcggaagtc gccggtatca tcccagttcg
     1321 atccatcgga gacaaacttc agtccgaggg ctttggctct ctcccggcag aggacgaaaa
     1381 gctccgtctt gcaatgataa cacctgctgg ggctgttctg gacgaagctg gggatcttca
     1441 gctcgttgga aaaaacttcc acgtaccgga cccccatttc ctcagccagt tttttggctt
     1501 ggtccacctc ggaagaggga taggtcgggg aggaagcgat cagggccatg gcacgatcgc
     1561 cgagctcttc gtgagccagc cgcaggagaa gggtgctgtc cacccctccc gaaaaggcca
     1621 ccaggaccga gcccatctcc cgcagaatct gtctggcttt ctccgctttg ctctccgaca
     1681 aaaccgagct tcctgaactt ttatgaggat ggtgggcgga attttggctc ccttttttgc
     1741 tcatgttctt tccttttctg ttcgaatatt cgaggtcgcg gccccccccc aggaagccaa
     1801 agtgtacgat cgtccctgcg gggttctccc tgtctgccct atttaattat atgagtcggg
     1861 gaatggattg tcaaaagaat gctcccctgg ctgctatagt cagagccgaa gaggcccgat
     1921 tccttcttcc gatgtagggc cggccgagcg gtcgccctta ccagaagcct aggatggaaa
     1981 cctgttctta tagacggcag tctcagaaaa accttgactt tccttcttcc tttctcatta
     2041 caataaatga aaacccgaat ggagggaccg cttggattcc cgaatggctg agagtttccg
     2101 ctccattccc tatggagtct acctcctgac ggcgcggaaa gggcccgatc ttttttcagt
     2161 cgttgtttcc tgggtgtccc aagtctcctt ctccccgccg cgcctgatgg tggctctacg
     2221 ccacaaccga agagtgattc cggcgattca ggaaagcaga tttttttctc tcagccttct
     2281 ctccacggac caaaaggccc tggtcgcccg ctttaaaggt ctgaatcctc ctgctgggcg
     2341 cggagatctc tgggaagaag cagactcctt tcatgctccg attctcaaag gctgcttggc
     2401 cgggtggcaa tgccgtcttt tttccacggt ggaggcggga gatcatttcc ttttcctggg
     2461 cgaagtggaa tccgcctccg gccggaaaga gggcttgccg cttaccacgc tggatttggg
     2521 gaaaacctac atcggcgaat tctgaggaga gggccgacaa atgaagagga acaacacgat
     2581 cgtcaaagtc ctggacattc tgcagaagga ataccccgat gcccgggtga ccctggattt
     2641 caaggacccc ctgcaactgc tcatcgccac tatcctggct gcccagtgca ccgatgagcg
     2701 ggtgaaccaa gtcaccaaag accttttcaa gaagtaccgg aaagcctcgg attacgccgg
     2761 ggcggacttg aagactctcg aggaagagat tcgctctacc ggttttttcc gcaacaaggc
     2821 gaaaagtatc gtcggctgtt gtcagatgct ggtacaaaaa ttcggcgggc aggttccccg
     2881 gacgctggag gagttgacct ctcttcccgg ggtaggccgg aagacggcca atatcctact
     2941 ggggaacgcc ttcaaccagc aggccattgc cgtggacacc cacgtgaaga gagtcactca
     3001 ccggcttggc tgggctaaat ccgaagaccc ggataagatt gaattcgaat tgatggaggt
     3061 cattccacag aaccgatgga ccctctcctg tcatcagatt gtctttcacg gccggagggt
     3121 ctgcatggcc aaaaaccccc agtgtcccac ttgcccggtg gcgaaactct gccccaagat
     3181 cggggtggcc caaaaggaat aaccctcccc ctgggaaaag gattgtaggg gcgacccgcc
     3241 gggtcgcctc tacgggacga ggggagagca gagatgagtc aataggagga aatgatgttc
     3301 ctgaaacagt tcgaggtgag cggcatggcg gtctttgcct atctcctggg agaagaaggc
     3361 ggaggccccg ggctggtcat cgacccggcg gacgatgccg accggattct ggctgaggcg
     3421 gaaaagaacc ggatcaaggt ccagtatatt ctcaacaccc acgcccatgt ggatcatgtc
     3481 atggggaacg aagagatgaa gcgtaaaacc ggagcgccca tcctcattca tgaggttgat
     3541 gctccatcct taaccaaaat ccccagagcc atgctggcca tgttcggcgg gcgcccctcc
     3601 cctgccgccg accagacagt gaaagacggg gatgtgatcc aggtaggcag cttgtccctg
     3661 aaagtccttc acacccccgg ccactctcca ggatccgtct gttttcatgg ggacggaatg
     3721 gtttttaccg gagacacgtt gtttgtggga ggagtgggac ggaccgacct tcccggaggc
     3781 tcctggccca tgatgctccg atccatccag accaagctcc tgatcctccc tgacgagacc
     3841 cttgtgctcc cgggccacaa ctacggagcc acgcccactt caacgatcaa gaacgagcgc
     3901 cttcacaatc cgttcttaaa atagtcctca acgggacgac gaggatgccc agcaggcttg
     3961 atccctcttc gactggcgat ccttctttcc cgcacttcac atttttaatt ttatttccag
     4021 atcccgaacc agcctttcga tctcctctcg ttcctccggg tccagcttat tctcagatag
     4081 acgtttcttg agggcaagca gttgctgctg gtcacgatat tcctcacagg ataaagccat
     4141 ctattgaatc ctcaccgatt tctgtagaac ttagactggc catttccggt ccaaaaatgc
     4201 ccttctaaag tttcttttga tgggtattat tgcccgagtc caccgtcatt tcaaaatatt
     4261 tgcctggtcc gatcccgccc tgaactttgg ttgggggatt acacaagtct acactgccgg
     4321 atacccataa aggttgacga ttacgcatag aacgcagcaa taaaaaggca gagctatgat
     4381 ggccctgcct tttctacatt atcaacccat ttcagcattt ttcaatactt gcaatttcag
     4441 gaaatgaagt acatctgttc cttgtaccct ttaccctcgc ggtaagaaga gtcatgtcgg
     4501 ccataggctc aaaacccgta aagtcgcgcc gggttgtcca ctaggatctt tttgcggatt
     4561 gccggatcag gtgcccacac gggcagcagg ttcaggacga gtccgtcatc aacctgccac
     4621 aagggggtca cgtccgtcgg cttgcgtccc gggggcgtca ccgcattggg atggggccag
     4681 ttggttcccc ctaggatgcg atcggggttc gcggcaatca gcgccttggc gaggggaagg
     4741 aagccagtat aatcatggcg cggtccttcc gggatcgata tcttgacata ggccttcccc
     4801 gtgcggacca ggtccaggat attatcaaag ccgggctggt ccacgctctt ctccggctcg
     4861 gcgccggcaa agtggtcgaa gacgagcggc acgggcgatg cggccagctg ggccttgatc
     4921 gcctcaatca tctccatggt ggcccacagc tgtatgtgcc agttgcgctt tttcatgcgc
     4981 tcggccacag cctggaacat ctggacggcg acggccgggt cggttatgcg gaccgtcttg
     5041 aggttaagcc ggatcccgcg aacgccaccg tggcccaggg cgtcaagctc gttatccgtt
     5101 gtcttgtcgt tgatcagggc gacgccgcgc gcatcgttcc cccgcgcctt tatgccccat
     5161 agcgtggccg agttatcgac gccataaatg ctcggtgtga c
//
LOCUS       DASPRR010000128         6749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1157180, whole genome shotgun sequence.
ACCESSION   DASPRR010000128 DASPRR010000000
VERSION     DASPRR010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157180"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VEL68_04705"
     CDS             complement(<1..518)
                     /locus_tag="VEL68_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35298.1"
                     /translation="MQYGLTEEQLLLRDTVRKLAKEKVEPGAGARDQKGEFSWEMVDL
                     LRENGLFGVDFPEEHGGAGAGLLALIIVCEELSKAEASTGMLPVAQELGTLPILLAAN
                     PDQKKKFLPALASGEKLSAFALTEASGGSDVAAFRSKAVKKGDYYILNGTKTFITNGG
                     VADTVAVYALTDP"
     gene            complement(856..2313)
                     /locus_tag="VEL68_04710"
     CDS             complement(856..2313)
                     /locus_tag="VEL68_04710"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXZ35299.1"
                     /translation="MAEPEKKQGVRTVKGGDIKPPWEVREMPDPPPMDFKGLMKWAGP
                     AIVLGALSVGGFEAYQAGYVAAQKWTGIYWVYIVSSFFQLLMNNEISRYTMATGETVL
                     QGFTRLKPAKFWGWVTAIFCIAQQGWPAWIGGAAAGFAALMGIATWQIVGMIAVGMVF
                     VVFSASKRVYNTLELIMYAAFVVANLGLALLTVIMTDWSVVKEVSWGWVAFGTIPAGI
                     TLTMIGPFLNQPAGGFWNFWHTYWVREKGMGMAKYFGHVTGFTAPAEEIRRTGYIFDT
                     DNPGELKKFRSWLKMNMITLVVFFVILGGIWFTYFASLAGYSAAVIYKMEVPSGIKIA
                     TVLAEIFKSAWGQFGYIMFAIILVFALFDSQFSVYDGIARMFADTVAVETHATDRRPY
                     RYFYFLMMGLLCAFGLIAVWMKTPYILWLIINWAGVACHAALTLIIIYLNAKLLPGPI
                     KPKGWIITLNVIWAVFLFIYFIVWTIYDNPLGIKL"
     gene            complement(2708..3688)
                     /locus_tag="VEL68_04715"
     CDS             complement(2708..3688)
                     /locus_tag="VEL68_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HXZ35300.1"
                     /translation="MAKRDYYEVLGVKKTASEEEIKKAFRKLAMKHHPDRNPGNKQAE
                     ERFKEINEAYAVLSDKQKRQQYDQFGPSGFSQRYSQEDIFRGFDISDLLKDLGFSFGR
                     AGGGGRRGRTQYGGLEDLFGGFGRGQAGGAQTGDFRDIFSGGGYQDQGPFGQKGQDIN
                     SELRLSFEEAARGAEKKVRFSKGNRIEEVSVKVPAGIESGKKLRLAGKGMEGAGGGQA
                     GDLYLKVNVAEHPVFKREGSDILVDKEIKISDAVLGTSIDVPTLEGDKQIKVAPGTQS
                     NSRIRLKGFGLPRLQEGGKGDQLVRIIIKYPRNLTERQKKLIEELKKEGL"
     gene            complement(3693..5453)
                     /locus_tag="VEL68_04720"
     CDS             complement(3693..5453)
                     /locus_tag="VEL68_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ35301.1"
                     /translation="MPAKMGKDIDIKEIQKDLSDYLTKKYGRKIQFAGFGPIPEPAGE
                     KVEEGEKEKQPGLSAIHFDMKPEELKAYLDGYLVKQDDAKEVLATKVCTHFNRIKMFE
                     MSRKYKQQESVGEIKNNIIMIGPTGVGKTFLVKLIARKIGVPFVKGDATKFSETGYVG
                     GDVEDLVRDLVYEAKGNVELAQYGIIYLDEVDKIAASPNLVGPDVSRSGVQRALLKPM
                     EETEVELKVPHDPVSQMEALMEFQKTGKREKKKINTKNILFIMSGAFNGLDEMVKKRL
                     NRQGIGFGAEIRSKDEKMEYLKKVKAEDLIEYGFESEFIGRLPVVTVFEHLEVDDLYK
                     ILRNPKSPIILGKKRDFKAYNIHLQFEDESLQRIAENAFQERTGARGLVSAVERVLLK
                     FEHTLPSTNIRHLVVTRAMVDDPAGELKKILDHADDPGQEAAFQELLAEEEKNLEALL
                     REKAEEWQERYGIRFSDERIQLITRRSVETQTDVDGVVEEIQAIHQAAREFAEKFSLR
                     NDLGVSFTEEAINRLVEKVWKEGLDPGDYLGKSFQNYEHGLKLIREKTGKREFSISAE
                     GMENPEQFLNGLIQETYRSE"
     gene            5802..6149
                     /gene="hypA"
                     /locus_tag="VEL68_04725"
     CDS             5802..6149
                     /gene="hypA"
                     /locus_tag="VEL68_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HXZ35302.1"
                     /translation="MHELSVTQSILDIALEYARKDQAKKIVAIQLQIGEITDFDDEWI
                     QRYFDFVSKGTMAEGAKLRITRIPAKLQCNSCSSVFPLDRASWNNQCPSCQSKDTQLI
                     SGREFRVEALEVD"
     gene            complement(6378..>6749)
                     /locus_tag="VEL68_04730"
     CDS             complement(6378..>6749)
                     /locus_tag="VEL68_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35303.1"
                     /translation="DAGGIPGEGREIFPSGNEDFILYQKSLEKLRPLDVDVICLARNG
                     AFLGQDAKEYVSRSDEEAEKMRLEIVRQFQGVPDPDEKLARLARERYDSLKTRDIPWD
                     VYLGLMRGVVKKILQYAEKSP"
BASE COUNT         1413 a         1954 c         1688 g         1694 t
ORIGIN      
        1 gggtcggtca gggcgtagac agcgaccgtg tcggccaccc cgccgttggt aatgaaagtc
       61 ttggtcccgt taaggatgta atagtcgcct ttctttaccg ctttactgcg gaaggcggcc
      121 acgtccgaac cgccgctggc ttcggtcaga gcaaaggccg acaatttctc ccccgaagcc
      181 agggccggca ggaatttctt tttctgatcc ggattggccg ccagtagaat gggcaaagtt
      241 cccaactcct gggccaccgg gagcatccca gtggaggcct ccgctttgga tagctcctcg
      301 cagacaatga tcagggctaa gagaccggcc ccggctccgc cgtgctcctc cggaaaatcg
      361 accccgaaaa ggccgttttc ccggagcagg tccaccatct cccagctgaa ttcccccttc
      421 tggtcccggg ccccggcccc cggctcaact ttctccttgg ccagcttcct caccgtatcg
      481 cggagtaata attgttcttc cgtcaatccg tactgcataa ccatctcctt tccccacgcc
      541 cttcctgcct tcctcggagc tccgaagaat tcccataaat cacccccgcc ctcaccctcc
      601 cccatcgagg gggagggaat ttttatctat ttcaattttt taccctctcc ccatgcggga
      661 gagggcaggg tgaggaaggt tagggtggag ggggttccac atattttttt aaaaaaaaag
      721 atatttctac accaaggtac ttctaggggg attgtagaaa aatccttaaa gaaatgtcca
      781 taaaaaaatg gagggatttt ccggggagga agagtcctcc ccggaattcg gttgagtggt
      841 cgaatgcgat gtcgtctaga gcttgatccc cagggggttg tcgtagattg tccagacgat
      901 gaaatagatg aacaggaaca ctgcccagat cacattcagg gtgatgatcc accccttcgg
      961 cttgatcggt ccgggcagga gcttggcgtt cagatagatg attatgaggg tgagggccgc
     1021 atggcacgca accccggccc agttgatgat gagccacagg atgtaggggg ttttcatcca
     1081 gacggcgatc aacccaaaag cgcataggag ccccatcatg aggaaataaa agtaccggta
     1141 cggtcgccgg tcggtggcgt gggtctccac cgccaccgtg tccgcaaaca tgcgggcgat
     1201 gccatcatag accgaaaact ggctgtcgaa cagggcgaaa accaggatga tggcaaacat
     1261 gatatagccg aactggcccc aggccgattt gaaaatctcc gccaggacgg tggcgatctt
     1321 gatcccggac gggacctcca tcttgtaaat gaccgccgca gaatacccgg ccagggaggc
     1381 gaagtaggtg aaccagatcc ctcccaggat aacgaagaag accaccaggg tgatcatatt
     1441 catcttcagc cagctgcgga acttcttcag ttcaccgggg ttatcggtgt cgaagatgta
     1501 cccggttcgg cgaatctcct cggccggtgc ggtgaagccc gtcacatggc cgaaatactt
     1561 ggccatcccc atgcccttct ctcgcaccca gtaggtgtgc cagaagttcc agaacccgcc
     1621 tgctggctgg ttcaggaacg ggccgatcat cgtgagggtg atcccggccg gaatggtccc
     1681 gaaggccacc cagccccagc tcacctcttt gaccactgac cagtccgtca ttatgacggt
     1741 gagtaacgcc aggcccaggt tggccaccac gaaggcggcg tacatgatca attccagcgt
     1801 gttatacacc cgcttgctgg cggagaagac tacgaacacc atcccaacag caatcattcc
     1861 cacgatctgc cacgtggcga tgcccatcag tgcggcgaag ccggcagcgg cgccgccgat
     1921 ccaggctggc cagccctgct gcgcgatgca gaagatggcg gtgacccagc cccagaactt
     1981 tgcgggtttc aggcgggtga aaccctggag gaccgtttcg ccggtggcca tggtgtagcg
     2041 cgagatctcg ttgttcatga ggagctggaa gaaggaggac actatgtaga cccagtagat
     2101 gcctgtccac ttttgggcgg cgacgtagcc ggcctggtag gcctcgaaac caccgaccga
     2161 cagggctccc aggacaatcg ccggtcccgc ccatttcatg agccccttaa aatccatggg
     2221 agggggatcg ggcatttccc gaacttccca gggaggtttg atatcccctc ccttcaccgt
     2281 acgaacgcct tgcttctttt ccggttctgc catttttcct tctcctttct tggatcagat
     2341 ttgacagaaa aagaaacacc aagacctcat acagcaaaat ccgccactgg ctgaattttc
     2401 ctcgcctgtt ttagactgga tttctcgggc aaatcttaag gaatgtcggg gatgaacccc
     2461 gtttctctca gcaacccaac ggtatggtag gaaaagcgga tttttttgtc aatagctatt
     2521 taaaaagagg ggggtagggg gtaatagtcg ggtagtcgcc tagtcgcaca gttctttagg
     2581 cttattgttt gatagtcgaa gtgaaaggat aatttcccat tccattttat ttatgtccat
     2641 gcagctgtga ggcaaaaaat aaccctcgac gatgcatcgt aatggactat acggctatgc
     2701 aactctatta aagaccttcc ttcttcaact cctcgatcag ctttttctgc ctctctgtca
     2761 gattcctggg atatttgata atgatgcgca ccaattggtc tccctttccc ccttcttgaa
     2821 ggcgggggag gccgaagccc ttgaggcgga tgcggctgtt gctctgcgtt ccgggtgcga
     2881 ccttgatttg tttgtctcct tccagggttg gaacatcgat ggaagtgccc agcacggcat
     2941 cggagatttt gatttccttg tcaacgagga tatcgctccc ctctcttttg aaaaccgggt
     3001 gctcggccac gttcaccttt agatacagat cgcccgcttg acctcccccg gcgccttcca
     3061 ttcctttacc cgccagccgc aatttcttcc cgctttcgat ccctgcgggg accttcacgg
     3121 atacttcttc gatcctgttc cccttggaga accttacctt tttttccgcc ccccgggcag
     3181 cctcttcgaa ggaaaggcgc aattccgaat tgatgtcctg acctttctgg ccgaagggcc
     3241 cctggtcctg gtaccctccc ccggagaaga tatcccggaa atccccggtc tgggctcccc
     3301 cggcctgtcc ccttccaaaa ccgccgaaga ggtcttcgag tcccccgtac tgggttctcc
     3361 cccgccgtcc tcctcctccg gcccggccga aggaaaagcc caggtccttc agcaggtcgc
     3421 tgatatcgaa cccgcggaaa atatcctctt gggagtagcg ctggctgaac cccgaaggcc
     3481 cgaactgatc gtattgctgg cgcttctgtt tgtcgctcag taccgcgtag gcctcgttga
     3541 tctccttgaa cctctcttcc gcctgcttat tcccggggtt gcggtccggg tgatgtttca
     3601 tggccagctt gcggaaggcc ttcttgattt cctcttccga ggcggtcttc ttgactccca
     3661 ggacttcgta ataatctctt ttggccattt cctcactcgc tgcgataggt ttcttgaatc
     3721 aatccgttca aaaactgctc cggattttcc atcccctcgg cggaaatcga gaactcgcgt
     3781 ttccccgtct tttcccggat aagcttcaac ccgtgctcat agttctggaa gctctttccc
     3841 aaataatcac ccggatccag cccttccttc catacctttt ccaccagccg gttgatggct
     3901 tcctcggtga aggacacccc cagatcattc cggagggaga atttctccgc aaattcccgg
     3961 gccgcctgat ggatggcctg aatctcttcc acgactccat ccacatcggt ctgcgtttcc
     4021 acggaccgac gggtgataag ctggatgcgt tcgtcggaaa accggatccc gtaccgttcc
     4081 tgccactctt ccgccttctc tcggagcaat gcctccaggt tcttttcctc ttcggccagg
     4141 agttcctgga aggccgcttc ttgcccggga tcgtcggcat ggtccaggat tttcttgagt
     4201 tcccccgcag ggtcatccac catggcccgg gttaccacca ggtggcggat gttcgtggag
     4261 ggcagggtgt gctcgaactt caaaaggacc cgctctacgg cgctcactag gccgcgggcc
     4321 ccggtccgtt cctggaaggc gttttccgca atccgctgga gggactcatc ctcgaattgc
     4381 aggtggatgt tgtaggcttt gaaatcccgt ttcttcccca ggatgatggg acttttgggg
     4441 ttgcggagaa ttttgtaaag gtcatctact tccaggtgtt cgaaaacggt aaccaccggc
     4501 agacggccga taaactccga ctcgaatccg tattcgatga ggtcctccgc cttgaccttc
     4561 ttgaggtact ccatcttctc atccttggac cgaatttcgg ctccgaaacc gattccctgc
     4621 cgattcagcc gttttttcac catctcatcc aggccattga aagcaccgct catgataaac
     4681 aggatattct tcgtattgat cttctttttt tcccgctttc ctgttttctg aaactccatg
     4741 agcgcttcca tctgggaaac cggatcgtgg gggaccttca attccacttc ggtctcttcc
     4801 atgggtttga gcagggcccg ctgaacgccg gaacgggaca cgtcgggtcc gaccaggttc
     4861 ggcgaggctg caatcttgtc cacttcatcc aggtaaataa tcccgtactg ggcgagttca
     4921 acatttccct tcgcctcata gaccaagtca cgcaccaggt cttctacatc tccgcccacg
     4981 tagcccgtct cgctgaactt ggtggcatcc cccttgacga aaggcacacc gattttcctg
     5041 gcgattagct taaccagaaa ggtctttccc actccggtcg gcccgatcat aatgatattg
     5101 ttcttgatct cgccgacgct ttcctgctgc ttatatttcc ggctcatctc aaacattttg
     5161 atccggttga aatgggtgca gaccttggtg gccaagactt ctttggcgtc atcctgcttg
     5221 accaggtatc cgtccaggta ggccttcagc tcctcgggct tcatgtcgaa atgaatggcc
     5281 gacagccccg gttgcttttc tttttcccct tcctcgactt tttcacccgc tggttcgggt
     5341 atgggcccga agccagcaaa ctgaatcttc cgcccgtatt tttttgtcag gtaatcgctg
     5401 agatccttct gtatctcttt gatatcaata tcttttccca ttttggctgg cataaccctc
     5461 catttctttc aactacgaga atattataat accttttccg tcagaatgcc actttcggtt
     5521 ctcggttccc cgggacccgc gctccaatat tttgcgcatt tctccttgat tccccggaaa
     5581 tttcctgatt aaatcacgac aaatctgata gccttcttaa atccccccct ttttaaaggg
     5641 gggaacgggg ggatttctgc ggccccctgg gttaaaggga gatgaatgga aatttggcgg
     5701 catttttcaa aaagctgaat ggttaccgtc tttgaatttt atttggaatt ttgcgtttac
     5761 gacggatttt cccgaatgcc cgaaagaatg gggtaacttc tatgcatgaa ctttccgtga
     5821 cccagagtat cttagatatt gccctcgaat atgcccgaaa ggaccaggcc aaaaagatcg
     5881 tggcgatcca gttacagatc ggtgaaatca ccgattttga cgacgagtgg attcagaggt
     5941 acttcgactt cgtgagcaag gggacgatgg ccgagggagc caaactaagg atcactcgaa
     6001 ttccggcgaa gcttcaatgc aactcctgtt cttccgtttt ccccctggac cgggccagtt
     6061 ggaacaacca atgtccctct tgccaaagca aggacaccca gctgatctcg gggagggagt
     6121 tccgggtgga ggcgttggag gtggattaag acagttatca gcgatcagct ttcagcggtc
     6181 agcaaaggca ctctttaagt taaattttgg ctgaaaactg aggctcctgc aaaagccact
     6241 taatccgtca ttccggcgaa agccggaatc cagaaatcaa tgaaaatcct ggattccggg
     6301 tcgcgcttcg cttgcccgga atgacaactt accctgtttt cgagattttg caagaacatc
     6361 aaatgacagc tgattcttta tgggctcttt tccgcgtatt gcaagatttt tttcactact
     6421 ccccgcatta gccccaggta gacatcccag gggatgtctc tggttttcag actgtcatac
     6481 ctctctcgag ccagtcgggc taatttttca tccgggtccg gtaccccttg aaactggcgg
     6541 acgatctcca gacgcatctt ttccgcttcc tcgtcggagc gggaaacata ctctttggca
     6601 tcctgcccaa gaaaggcgcc attgcgggca aggcaaatga catccacgtc gagaggcctc
     6661 agtttttcaa gcgacttctg gtaaaggatg aagtcttcat tgcccgaggg gaagatctct
     6721 cttccttccc cgggaatgcc tccggcatc
//
LOCUS       DASPRR010000129         7438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1808710, whole genome shotgun sequence.
ACCESSION   DASPRR010000129 DASPRR010000000
VERSION     DASPRR010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808710"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="ccsB"
                     /locus_tag="VEL68_04735"
     CDS             <1..610
                     /gene="ccsB"
                     /locus_tag="VEL68_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HXZ35304.1"
                     /translation="IALLYLLWERKLKSRVIGAFALPFAFAFNAYASLASGVSAKIDP
                     LIPALQSNWLHAHVITCFLAYASFALSCAISVIYLVKLRKNGKIEKEDRMTGLFPALD
                     SLDDLIYRTIVVGFPLLTIGIVTGAAWANYAWGAYWSWDPKETWSLITWFAYAIFLHA
                     RITRDWRGKRAAILSIVGFIAVIFTYFGVNYLLSGLHSYGSS"
     gene            878..1252
                     /locus_tag="VEL68_04740"
     CDS             878..1252
                     /locus_tag="VEL68_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HXZ35305.1"
                     /translation="MSGKIKIKAGNVEADAVLNDSPTAKKIWEALPIEARGNTWGDEI
                     YFAIPVKSSLEKDAKELVEVGDLGYWPSGNAFCIFFGPTPVSRGNEVRPASAVNVIGK
                     IIADAKVFKKVADGVKVRIEKA"
     gene            1400..1621
                     /locus_tag="VEL68_04745"
     CDS             1400..1621
                     /locus_tag="VEL68_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXZ35306.1"
                     /translation="MPIFEYHCPKCDQDFEKIVFNDKTKVQCPKCHNGKVSKKVSAFS
                     FKSGSKFVASSSKAGGCSGCSSHHCSTCH"
     gene            1654..2274
                     /locus_tag="VEL68_04750"
     CDS             1654..2274
                     /locus_tag="VEL68_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXZ35307.1"
                     /translation="MERAKGAIKRTFLTGLVVVVPILITAFTLYWLFRVLDGILSPII
                     DQILGRDIPGLGILTEIVIIFLVGVLATNVLGSRILHWFQTLLMRAPVVKNIYPTIKQ
                     LVEAFHPSSSSSFKKVVLVEYPKMGTYALGFMTSEVSMKGTPQPRRLFSVYLPTNNLY
                     LGNVALFKEEDLIVTDFTVEEGLKIVLSGGTAFPPTIQKQNQTRKP"
     gene            2400..3809
                     /gene="amrB"
                     /locus_tag="VEL68_04755"
     CDS             2400..3809
                     /gene="amrB"
                     /locus_tag="VEL68_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXZ35308.1"
                     /translation="MSEEVRKPAVAGSFYPGDSKTLSREVRDYLSKAAKEEMAGEIVA
                     LVSPHAGYMYSGLVAAHAFKAVEGMKFDAVVILAPSHRVPFQGASVYDRGGYETPLGV
                     LPIEKELCQKLKSESNLIQAFPQAHSQEHSLEVQLPFLQEVLGKFPLVPMVMGSQDYR
                     SCENVGKAIARAVKGKKVLLVASTDLSHYHPYARAVQLDRIILDDIQAFDAQKLSRDL
                     DTGKGEACGGGPVVSVMVAAKELGANRAQVLKYMNSGDVTGDRSGVVGYAAAVFLRNS
                     AALGKEPDRKKAGISLGLTEEEKKTLRQIAQSAIERRLKGEKPSKINLASEQLKGNRG
                     AFVSLHTHGQLRGCVGTIQPNRPLHQVVEEMAIAAAFEDSRFSPLSTAELKDLDLEIS
                     VLTPLERVQDVDEIKVGMHGLYIKKGFYAGLLLPQVATEYQWDRVRFLEETCRKAGLP
                     KNAWMDKGTEIYLFSADIF"
     gene            3901..4239
                     /locus_tag="VEL68_04760"
     CDS             3901..4239
                     /locus_tag="VEL68_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HXZ35309.1"
                     /translation="MPIYEYECQNCEAVSGFLVMKKEDQEKIACLACGSKKMSKVVSR
                     VTYHRSEDDRLAEFDSQKPQGEEFYKDSRNIGLWAKKRAKELGADLGPQFDEIVENAR
                     TGKILKKYGL"
     gene            4236..5387
                     /gene="amrS"
                     /locus_tag="VEL68_04765"
     CDS             4236..5387
                     /gene="amrS"
                     /locus_tag="VEL68_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXZ35310.1"
                     /translation="MIDELKKKPLFLSRRQFLKTTACSALLWSVWGFSPPAFAQRGKK
                     GLIEPKLSPHFQSLKDGKIQCQLCPRECIVPKGGRGFCGVRENREGKYYSLVYGNPCA
                     VHVDPVEKKPFYHLLPASTSFSIATAGCNFRCKFCQNWEISQVTPEDTYNYDLPPERV
                     VELAKKAGSRSIAYTYVEPTIFFEYMLDTAKLARKEGILNVYHSNGFINPGPLRELCK
                     FLDAANIDLKGFKEDYYGSMTQGRLAPVLRTLKTLKTEGVHVEVTNLVIPAQNDDPDI
                     VGEMCSWVKTELGPDTPIHFSRFYPLYKLRNLPPTPVTTLEINRKIALEAGLEYAYIG
                     NVPGHEAERTYCPQCKKLIIFRQGYSIGEVNLNKGKCKFCGKPISGIWT"
     gene            5537..5986
                     /locus_tag="VEL68_04770"
     CDS             5537..5986
                     /locus_tag="VEL68_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35311.1"
                     /translation="MIKDDRSYHLRLQEFCDCFMETDFRKELEQASKGVSGDPGGDLD
                     ELALKFLGLTLLYGANEEANKISLQKSNRGKVLFSVEARGNYQLIPPSGNLADKIFSV
                     ARSMVHLDKDQGKIPFSLGLRNSSLEINLEFARSEGADSLTFIFPKK"
     gene            complement(6244..7194)
                     /locus_tag="VEL68_04775"
     CDS             complement(6244..7194)
                     /locus_tag="VEL68_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HXZ35312.1"
                     /translation="MENRIRLGISACLLGENVRFDGGHKLDHFLTDTLGQYVEYVPVC
                     PEVECGFGVPRESFRLVGDPENPRLVTTRTNIDHTERMVQWARKRVQELGKEDLCGYI
                     FKSGSPSSGMERVKVYNRNGVPAKVGVGVFARIFMEHFPLLPVEEEGRLHDPKLRENF
                     IERVFALKRWRETISKKQSLGGLVDFHTKNKLLILSHSPATHRAMGKLVAKGKQIPLE
                     TLYERYQTLLLQALQLRTTAKKNANVLEHMLGYFKKQLSPNEKQEMLEVINQYRQSLF
                     PLIVPLTLMNHYVRKYDQPYLKEQSYINPHPVELQLRNHV"
BASE COUNT         1749 a         1948 c         2012 g         1729 t
ORIGIN      
        1 tatcgccctt ctctacctgc tctgggaacg gaaactgaaa tcccgggtga tcggggcctt
       61 tgctctccct ttcgcctttg catttaacgc ctatgcttcc ttggcctcgg gggtctccgc
      121 caaaatcgat cccctaatcc ctgctctcca gagcaactgg ctccatgccc acgtgataac
      181 ctgtttcctc gcgtatgcct ccttcgccct gtcctgcgcg atcagtgtca tttacctggt
      241 caagctcagg aaaaatggga agatagaaaa agaggaccga atgaccggtc tctttcccgc
      301 cctggattct ctggatgatt tgatttatag gactattgtg gtcggtttcc cacttctgac
      361 catcggcatc gtcaccggag cggcttgggc caattacgcc tggggcgcct actggagctg
      421 ggaccccaaa gagacctggt ctttaatcac ctggtttgcc tacgccatct ttctccacgc
      481 ccgcatcacc cgggattggc ggggcaaacg cgcggcgatt ctctccatcg ttggattcat
      541 agctgtcatc ttcacctact tcggagtcaa ttacctcctt tcggggctcc acagctacgg
      601 ttcctcataa ctgacttggg atgaataagt tgaatttgac atccaagccg tcattcatta
      661 caatcatacc gaagtggaga tgctccatca tttcttgaat cgctgaattc cctgcccgat
      721 tcagggtaaa aagagggctg cggtggaaac gggtaagaga ggccctattt ctctggaatg
      781 aagaggaatg gggcttttta ttcccgatgg gtctagggtc tgagaatttt caaaccggag
      841 ggccggcaca aaatgaatga ttccacggga ggaacaaatg agcgggaaaa ttaaaatcaa
      901 agcggggaat gtggaagcag atgcggttct gaacgattcc cccacggcca agaaaatctg
      961 ggaagctcta cccatcgaag cccggggaaa cacctgggga gatgagattt actttgccat
     1021 cccggtgaag tcctccttgg agaaagatgc caaagaactc gttgaggttg gggacctagg
     1081 ctactggccc agcgggaacg ccttctgcat cttcttcggg cctaccccgg tgagccgggg
     1141 gaacgaggtg cgcccggcga gcgcggtaaa cgtaatcggg aaaatcatcg cagatgccaa
     1201 ggtcttcaag aaagtcgccg acggggtcaa ggtgaggatt gagaaggctt aatacattct
     1261 gtatgcggat tttggagtgc ggaagcggaa gcgggatttt tcagggagat tgaatttttc
     1321 ctttttctcc gaattccgca tttgcattgc cttcccccca tccctttgtt ataattaaag
     1381 aaaaaaaagg agttcgggaa tgccgatttt cgaataccac tgccctaaat gcgaccagga
     1441 cttcgaaaaa atcgtcttca atgacaagac caaagtccag tgccccaagt gccacaacgg
     1501 caaagtgagc aagaaagtct ctgctttcag cttcaagagc ggttccaagt ttgtcgcctc
     1561 ttcctccaaa gcggggggat gcagcggttg ctccagccac cattgcagta cctgccatta
     1621 atctgttttt gatggtttta ggttggaaat tccatggaaa gagctaaggg ggcgatcaaa
     1681 aggacctttt tgaccgggct ggtggtggtc gtccccatcc tcatcaccgc ctttactctc
     1741 tactggctct tccgagtcct tgacggaatc ctgagtccca tcatcgacca gatcctgggt
     1801 agagatattc ccgggctggg aattctcacc gaaatcgtga tcatcttctt ggtgggcgtt
     1861 ctggccacca atgtcctggg ttcccggatc ttgcactggt tccagaccct gctgatgcgg
     1921 gctcccgtgg tcaagaatat ttatcccacc atcaagcagc tggtggaggc cttccacccc
     1981 tccagcagtt cgtcctttaa gaaggtcgtt ctggtggaat acccgaaaat ggggacctat
     2041 gccctaggat tcatgaccag tgaggtttcc atgaagggca ccccgcagcc tcgacgcctc
     2101 ttttccgtct accttcccac caacaatctg taccttggaa acgtggccct gttcaaggaa
     2161 gaagatttga tcgttaccga tttcaccgtc gaagagggtt taaaaatcgt tctttccggg
     2221 gggacggctt ttcccccaac cattcagaaa cagaaccaaa cccgaaagcc atgagaaata
     2281 ttttccatgc ccgggtgaat tggttttccc ttttcattct ggcgattgcg gcaagctggg
     2341 tccttttagc ctctgcaaac ggtcaggagc ggggcagagc caaagaggaa gggaaagcta
     2401 tgagcgaaga ggtgaggaag cctgcagtgg ctggttcctt ctaccccggg gacagcaaaa
     2461 ccctgtcccg ggaggtgcgg gactaccttt ctaaggcagc caaagaggaa atggccgggg
     2521 agatcgtcgc cctggtttct ccccacgccg gttacatgta ttccggcttg gtggcagccc
     2581 atgccttcaa ggccgtggag gggatgaagt tcgacgcggt ggtgatcctg gcccccagcc
     2641 accgagttcc ctttcaagga gcctcggtct atgaccgggg aggctatgaa acccctttgg
     2701 gggtcttgcc gatcgagaag gagctttgcc agaagcttaa aagcgaaagc aacctcatcc
     2761 aagcttttcc ccaggcccat tcgcaagaac attccctgga agtccaactg cccttcctcc
     2821 aggaggtctt ggggaaattc cccctcgtgc ccatggttat gggaagccag gactaccgtt
     2881 cctgtgagaa tgtgggcaag gccattgccc gggcggtgaa ggggaagaag gtcttgctcg
     2941 tggccagcac cgacctctcc cactatcacc cctacgccag ggccgttcaa ctggacagga
     3001 tcattctgga cgacatccag gcgttcgatg cccagaaatt gagccgggat ttggataccg
     3061 gaaagggcga agcctgcggc ggggggccgg tcgtgtcagt gatggtggcg gcgaaagagt
     3121 tgggagccaa tcgggcccag gtcttgaagt acatgaactc aggggatgtc accggagacc
     3181 gctctggggt ggtgggatac gcagccgcgg tcttcctccg gaattccgca gctttgggga
     3241 aagagccgga tcggaaaaaa gccggaatca gcctgggatt gaccgaggag gaaaagaaaa
     3301 ctcttcggca gatcgctcag tcagcgattg aaagacgatt aaagggggag aaaccttcta
     3361 agatcaacct tgccagcgag cagttgaaag gaaaccgggg ggcttttgtc tcccttcata
     3421 cgcacggtca gctgaggggc tgcgttggaa ccattcaacc gaaccgtccc ctccaccagg
     3481 tggttgaaga aatggccata gccgcggcct ttgaagacag ccgcttttcc cctctttcga
     3541 ccgctgagct gaaggacctg gatttggaga tttctgtcct gactcccctt gaaagggtcc
     3601 aggatgtcga cgagattaag gtgggaatgc acgggctcta catcaagaag ggtttctatg
     3661 ccggccttct gcttccccag gtggctacgg agtaccaatg ggaccgggta agattcctgg
     3721 aggagacctg ccggaaagcg ggcctgccga agaatgcctg gatggataaa gggaccgaaa
     3781 tctacctgtt ctcggcggac atcttttagt ttttcaggat gaggttaaaa aaaccaacct
     3841 ttgtacttac acttacactt tcactagtca ctgaccctgt ctttggggga ggttttagcc
     3901 gtgcccattt atgaatatga atgccaaaac tgcgaggccg tcagcggctt cttggtgatg
     3961 aaaaaagaag accaggagaa aatcgcctgt ctggcgtgtg gaagcaagaa gatgtccaag
     4021 gtagtttccc gggtgaccta ccaccgttcc gaagacgacc gccttgccga atttgacagt
     4081 cagaaacccc agggcgagga gttctataag gactcccgga atatcggttt gtgggccaag
     4141 aagcgggcca aagaacttgg cgcagacctg gggccccaat tcgatgaaat tgtggagaac
     4201 gcacgcacgg gaaaaattct aaaaaagtac ggcctatgat cgatgaattg aagaaaaaac
     4261 ccttgttcct ctcccgccgt caattcttaa agacaaccgc ctgcagcgcc ctcctatggt
     4321 cggtctgggg cttttctccc ccggcctttg cccagagggg aaagaaaggg ctcattgaac
     4381 ccaagctttc cccccatttc cagtccttga aggatggaaa aatccaatgc cagctctgcc
     4441 ctcgggagtg catcgttccc aagggaggaa ggggattttg cggggtccgg gaaaaccgag
     4501 aggggaaata ttacagcttg gtctacggca atccctgtgc cgtccacgtg gacccggtgg
     4561 agaagaagcc tttttaccat ctccttccgg cctcgacctc gttttccatt gccaccgctg
     4621 ggtgcaattt tcgctgcaaa ttctgccaga actgggaaat ctctcaggtt accccggagg
     4681 atacgtacaa ctatgatctc cctccggagc gggtggtgga gttggccaag aaagccggct
     4741 cgcgttccat tgcctacacc tatgtggagc cgactatttt cttcgaatac atgctggaca
     4801 ctgccaaact ggccagaaaa gaagggatcc tcaatgtcta ccattccaac gggttcatta
     4861 acccaggacc gctgagagag ctctgcaaat ttctggacgc ggcgaatata gacctgaagg
     4921 gttttaaaga ggactactat ggctccatga cccaggggcg actggctccc gtcctccgaa
     4981 cccttaaaac gctgaagacg gagggggttc acgtagaggt caccaacctg gttattcccg
     5041 cccagaatga cgacccggac atagttgggg agatgtgctc ctgggttaaa accgaactgg
     5101 gcccggatac cccgatccat ttctctcgtt tctaccccct ctataagctt cgcaaccttc
     5161 cacccactcc agtcacaacc ttagaaataa atcgcaagat cgccttggag gcggggctcg
     5221 aatatgccta tatcggcaat gtgcccggcc acgaagcgga acggacctac tgcccccaat
     5281 gcaaaaaatt aatcatcttt cgccagggtt actccatcgg cgaggtcaat ttgaacaagg
     5341 gaaaatgcaa gttttgcggc aagccgatct ccggaatttg gacttaaggc ctattctcca
     5401 ttttgagaaa aagagccatg gaaattcaat agggaggggc attccgcgaa ccgcttttga
     5461 tgcttttgga aaaggttggc ggagcttgaa tcgtttccca agaaatttcc atcctaaaaa
     5521 ataggagggc aattatatga tcaaagacga ccggagttac cacctgaggc tgcaggaatt
     5581 ctgcgattgt ttcatggaga cggactttcg caaggagctg gagcaggcca gcaaaggagt
     5641 ttccggcgat cccggcgggg acctggatga acttgcacta aaatttttgg ggttgacttt
     5701 gctctatggg gccaatgagg aggcgaacaa aatttccctc cagaagtcca accggggaaa
     5761 agtcctgttc tctgtagaag cgcgtggaaa ttatcagctt atcccgccca gcgggaatct
     5821 ggcggacaaa attttttcag tggcccgttc catggtccat ctggacaaag accagggcaa
     5881 aattcccttt tctctgggtt taagaaacag cagcctggaa atcaacctgg aatttgcccg
     5941 gagtgaaggg gctgattctc taacttttat tttccccaaa aagtaattca acaactgttt
     6001 cgcccgcaga gggcagtgcc cggcaagttc caaccgaaag aataaccttt ccacgagacc
     6061 gcgcgccttc cataaatctc ccccgcctcc ctccttatca agggaggcga aggggggact
     6121 tgtgggatcc catttcggcc tgaggaaaaa ttttccagaa aggtggctgg gcgatttttt
     6181 ttgcggctct gccgcgctgc gatctctgca gcgatgagcc cgccgtccgc attgcgcatt
     6241 caatcagacg tggttcctta gctgcagctc gacggggtgg ggatttatat aagactgctc
     6301 cttgagatag ggctgatcgt attttcgaac gtagtggttc atcagggtta ggggaacgat
     6361 cagcgggaat agggactggc ggtactggtt gatgacttcg agcatctcct gcttttcgtt
     6421 cggtgaaagc tgtttcttga aatacccgag catgtgctcc agcacgttgg cgtttttctt
     6481 ggcggtggtt ctcagttgca gggcttggag caatagggtt tgatatctct cataaagcgt
     6541 ctcaagggga atctgtttcc cctttgcgac aagttttccc atggcgcggt gggttgcggg
     6601 actgtgggaa agaattaata atttattctt ggtgtgaaag tcgaccaggc cgcccaggct
     6661 ctgctttttg gatatcgttt cccgccatct cttcaaagca aagacccgtt cgatgaaatt
     6721 ctcgcgcagt ttgggatcgt ggaggcgccc ttcttcttct acggggagaa ggggaaagtg
     6781 ttccatgaaa attcgggcga agacccccac cccgaccttg gccggcactc cattgcggtt
     6841 gtagaccttg actcgttcca tgccgctgct tggagagccg cttttgaaga tgtaaccgca
     6901 caggtcttcc tttcccagtt cctgcacccg ctttcgggcc cactgaacca tgcgctccgt
     6961 gtggtcgata ttggtccggg tcgtgaccag gcggggattc tcgggatccc caaccaggcg
     7021 aaaagattcc cggggaactc cgaaaccgca ttccacttca ggacagaccg gaacgtattc
     7081 cacatactga cctaaagtat ccgtgaggaa gtggtccagt ttgtggccgc cgtcgaaccg
     7141 gacattttcc cccaggagac aggcactgat tcccagccgg atcctattct ccaccttcca
     7201 atgcctcccg attgaaggga aataatatgc cttctattat aagcgatccg gggaaatcga
     7261 ggaatgccga ttgcctggtg aacctccccg cccacagggc ggaaagattc actaccccag
     7321 ggaaggaaac aagtcaatcg ttttgctagt gtcgcgtccc ctaagttcag tgaataaatt
     7381 taccaggagg ggggcggaga aacgatgcgc gccgccaaaa tcggccggga aatggcct
//
LOCUS       DASPRR010000130        16726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_349354, whole genome shotgun sequence.
ACCESSION   DASPRR010000130 DASPRR010000000
VERSION     DASPRR010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16726)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16726
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349354"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /gene="pgi"
                     /locus_tag="VEL68_04780"
     CDS             <1..670
                     /gene="pgi"
                     /locus_tag="VEL68_04780"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252044.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXZ35313.1"
                     /translation="AILPYDQSMHRFPAYFQQGNMESNGKSVDRKGERVSYQTGPIIW
                     GEPGTNGQHAFYQLVHQGTKLIPADFLAPTISHNPIGDHHSILLSNFFAQTEALMNGK
                     TKEEVIGELRREGKPEEEIQRLWPYRIFEGNRPTNSVLFQKLTPRVLGSLIAMYEHKI
                     FVQGIIWNIFSFDQWGVELGKQLAGKILPELAEDRPVHSHDSSTNGLINTFKEMRKDG
                     KGNE"
     gene            663..2717
                     /locus_tag="VEL68_04785"
     CDS             663..2717
                     /locus_tag="VEL68_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXZ35314.1"
                     /translation="MNNLSSAEKPKGWFPRLISGNRTAFALLPALVSLVLAYRARLTY
                     GLFTHPIKPFDFSPGAHPVRFTLGFGDSDLFLILSCFFLFLLLSQGRFLLKSRKVFLP
                     FQVAGLVLLHLILGSLLFLHLGHFRFLFDAQTGFDFGVILESVYSVSTIGTLKLLEVR
                     DWLFVLFPLAIFWVVWLVPPAARAWVAKAFLAGVVFWGAFSLLGPPEKKNTVPAELRV
                     NPGIFFLSDMAEHLGRSPIRDRTGIRVQETESGFKPAGPLNSHPVRAVEDLPPQANHP
                     WNVVFLVLESVGTRYMQEGYGGSPVPMPFLNRLAGEGWFLKNHFTPSNVSTKADFSIF
                     SGLYDFFQRGTLGVRQDVQIPSLYNFLGPKYDSFLVSPSSSAWYFPLPFLRNGGLREI
                     HTYENLNFKIREELSTLGRYIARDEIQTVDFFLQRISRAREPFLGMYVSFVAHFPYFD
                     YGPEYRIVESDGKSISRYINNLYLLDRLVQRIFEHLKKRGLLERTILVIVGDHGQAFG
                     QHHPDNYLHYRYSYNVNLEAPVIFYQPALFKPRSFNVPTNHTDILPTLLDALRISYNP
                     SLLDGESLFHPLKRKYIFFYGHEESISSLSTDGIKVQVSLKKNRCWAFDLRADPDEKT
                     PLDCGPYQSQLEALRNFVRDHDTNLAQYNACNREGKDFQGQRHPALAAPPPGREEKKG
                     KG"
     gene            complement(2749..4185)
                     /gene="pyk"
                     /locus_tag="VEL68_04790"
     CDS             complement(2749..4185)
                     /gene="pyk"
                     /locus_tag="VEL68_04790"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXZ35315.1"
                     /translation="MKLPPHKTKIVCTIGPASESPEMLGKMIRAGMNVARLNFSHGDF
                     PGHKRVIENLRAAAREAGYRLAILADLPGPKIRIGQFGKEPIELKPGETFTLTTEEVL
                     GDSQRVSVSFSTLPQSVKAGDMIFLNDGFIQLEVLGIKGREVLCRVIVGGELRSRKGL
                     NLPGIDLGITAFTDHDRRCLQFALENGVDAVSQSFVESGKDLNAVRQAASSLGFSPFI
                     IAKVERSRALSHIDEILDAADGIMIARGDLGVEIPIEQIALVQKQLMRKANLLGKPVI
                     TATQMLESMTDNRRPTRAEATDVANAILDGTDCVMLSGESAMGKYPVESAAMLAKIAA
                     AIEPYRPAHYIRETLQGVGRGDRTRLTDLIALSVETTLERISPAAVFVPTHSGATARS
                     IARFRPPVWITAVSSQESTCQRLQFSYGVYPVHEPDHPEDWNAFARKWLQDNEVKGNL
                     VVLTEGPSVKHPEANNRMEIIDLGRIVA"
     gene            complement(4263..6383)
                     /locus_tag="VEL68_04795"
     CDS             complement(4263..6383)
                     /locus_tag="VEL68_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXZ35316.1"
                     /translation="MVKTKKPSPKSRPRTGKTPQEKIQELTRKNTRLQRKVEEFREKA
                     RQFEEIIRFEKVLSEISTRLVHLLADQIEREIELGLRRVVKFLGVDRGSVYIFSGDRT
                     RIFRKYFWGMEGIPESAPVLEDQKFPWLSKKLQKGEMLVIPRVDDLPPEAAKDREELK
                     KFRNLSSLVIPLAVGKNVFGAVTFGAVRAERNWPGGLIQRVRLVGEIFASALARKQSE
                     EALRQAELEYRMVAEFPYDWEYWSNPDGTIRYMSPSCRRITGYSPDEFVNRPSLIREI
                     ILPIDRGAWDQHFRESRRGVLAHEIQFRIRKPDGQVRWIEHACQSVRSPQGEFLGYRV
                     SNRDVTERRMAEEAARKKDESLAEAQRIAHMGNWHWNIETNELAWSDEVYRIFGLHPQ
                     EFGATYNAFLEFVHAGDREAVKEAVTRSLADPSILYGIEHRIVRPDHSERIVHERGEV
                     TFDDGGRPIRMIGTVQDITERKQAEQMLQRSQEELRRLSVQLLSVQEEERKRIARELH
                     DGIGQSLSAMKFLLENRLHWMEKNSSPGNLRDLESMIPMIQNAIEEVRRIQSDLRPPI
                     LDDLGILATISWFCREYQGIYGSIRVQRQLEVEEDEVPPPLKTVIYRVLQEAMNNVAK
                     HSQATKILLSLRKVNDCLELVIQDNGVGVELDRIISRKSEKYGFGLSSMRERTELSGG
                     FFSVQSGKGKGTTIQASWKVAAEA"
     gene            complement(6483..8114)
                     /gene="ggt"
                     /locus_tag="VEL68_04800"
     CDS             complement(6483..8114)
                     /gene="ggt"
                     /locus_tag="VEL68_04800"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXZ35317.1"
                     /translation="MFKTPETFPQRYRPLIMAPHGMVASPHYLASQAGVDILKKGGTA
                     VDAAIATNSVLNVVYPHMCGIGGDAFWLVYDAAKKDLAFLNASGRSAYAATIETYKKA
                     GMNSMPVRGPLTVTVPGAVDGWFEAHRRYGRLPMAALLETAIAYSRRGYPISHVLSFK
                     THEAVSELSSYSTSKGLFLPGGKAPRPGDVLTNPGLASSLEKIAREGRDVFYQGEIGR
                     EIVRFSRENGGLLTERDFLEHRSDWGKPVSTQYRGYTVYETAPNSQGLTALLILNLLE
                     GYSLASMGYQSSDHLHFMVEAKKVAFADRNRYISDPEKVEIPMDDLLSKDYANKRRAL
                     IQKDRAVDPQAISPGIFGRDTIYLCTVDEAGNAVSLIQSLYYSFGSAVVAGNTGILLQ
                     NRGAYFSLNPDHVNCLQPHKRTFHTLMASMTFRDGKPLLVFGTSGADGQPQTHIQVMT
                     GVFDFGLDIQAAIEAPRWVAGRYLLHHSEGLLMMEGRFPAPVVEELRKRGHQIQIVDG
                     IDQSMGSAHGIMVHPENGLRMGGSDPRCDGAAIGY"
     gene            complement(8314..10179)
                     /gene="uvrC"
                     /locus_tag="VEL68_04805"
     CDS             complement(8314..10179)
                     /gene="uvrC"
                     /locus_tag="VEL68_04805"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXZ35318.1"
                     /translation="MNLEDQIQSLPTSPGVYLMKDQAGTVLYIGKAKNLRSRVRTYFG
                     KSGDARLSLRFLMPKVRQVETFLTDTEKEALILENTLIKKHKPRHNIDLKDDKTYFSL
                     RFNLEEEFPKLTLVRKIKRDGARYFGPYASSAAVKETLRVLRHLFPLRTCNDANFRNR
                     SRPCLNFQIKKCLGPCCGMVSSEKYADLVQEVILFLEGKNSQLIKLLQERMTEASEAL
                     NFEEAARLRDQIAAIEQTLEKQKAVSQTPNDQDVFAFHRRGNAWEFQVLFFRRGILVG
                     NKAFHFPRLNLPEEEALSAFLRQYYAEEPSIPHEILLPLPIEDERLLAEWLSEKKGVR
                     VEVHAPQRGEKKSLVEMAGKNAENSFQKRVGDTETLALILQELKEKLRLKTVPHRAEC
                     FDISNLFGTLAVGSMVSFLDGRPEPSHYRHFKVHAPSFPDDYAMMYEILRRRYGKLEP
                     GGEKPNLLIVDGGKGQLNVALAVLAELGLQDISAVGLAKDKGPSLRKIAEKTADKVYL
                     PNVKDPVLLGRSASLRYLQRIRDEAHRFAIRYHKKLRGKHGLRTLLDDIPGIGEVKKK
                     ALLKEFGSPQKIQEASPDRLSQVGPMTHRDAQTVFEFFHPSSPAASSGEEGEGTG"
     gene            complement(10276..12291)
                     /gene="uvrB"
                     /locus_tag="VEL68_04810"
     CDS             complement(10276..12291)
                     /gene="uvrB"
                     /locus_tag="VEL68_04810"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXZ35319.1"
                     /translation="MPDFKIVSPFKPQGDQPQAIEQLVQGVKKGIAHQVLLGVTGSGK
                     TFTMARVIERVQKPTLVIAPNKTLAAQLFQEFRSLFPDNAVEYFISYYDYYQPEAYIP
                     TTDTYIEKDASINDLIDKMRHSATHSVLTRRDVIVVASVSCIYGLGSPETYQGMIVLM
                     EDQKETSRDEVLARLVEIQYERNDYDFHRGTFRVRGDVLEVFPAYEENTAIRVEFFGD
                     LVDSISEIDPLRGKVLRKLKQITIFPGSHYVTPQDRLRRAIQSIREELVERLAFFESQ
                     KKLLEAQRLEQRTNYDIEMLQEMGYCTGIENYSRHLDGRKPGEPPYTLLDYFPKDLLY
                     FIDESHITIPQLNGMYWGDRTRKETLAEFGFRLPSALDNRPLSFEEFEARVPQMVYVS
                     ATPAAYEMKKSRGRVVEQIIRPTGLIDPQVTVKPARYQVDDLLNEIQARVARGERVLV
                     TTLTKRMAEDLTSYYADLGIKVKYLHSDIQALERTEILRDLRLGKFDVLIGINLLREG
                     LDLPEVSLVAILDADKEGFLRSGTSLIQTFGRAARNVGGQVILYADQETESMKKAIRE
                     TDRRRKVQEDYNRKNRITPESIRKAIPDILQSIYEADYVTVPMAAEKPEEYVSLFEVP
                     KLIVRLKKEMREAASRLDFERAAELRDRIQALQKKELDTREGRAAEA"
     gene            complement(12517..13965)
                     /locus_tag="VEL68_04815"
     CDS             complement(12517..13965)
                     /locus_tag="VEL68_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ35320.1"
                     /translation="MAKIFPSGERKNPSLEKLFYPDSVAVVGASPEVKGDRFPFFQAF
                     LKAGFPGRLYPVNPNSPEIGGRKAFSRLQEIPEPIDLAIITLRAPKVPPIVADCVRKG
                     VRFAVIFSSGFTEGGHPELEEEIKALVCQGPTRLVGPNCIGPYCPESGVTFAIEEKAA
                     EPGNVGFVSQSGGHSITYKTLASSRGIHFNKILSLGNQCDLTIQEVLRYYAGDPQIRV
                     ICAYLEQARGGEEFARSLREVAHKKPLIVWKGGATEAGARAAFSHTGAMASHSGVWQK
                     AAHQLGVILVEDLEEMADATFSCLTLDLPRSPRVGIVVVGGGSSVEMTDICARNSLEI
                     PILGKETQEKIAKNIPQANTSCKNPVDLGFMGFFPQVYSQSIRLAATDPNIDVLLLYQ
                     ITEYFQQFTPHLDWPANIAAEMAKVNRELQKPMVIVIPPLEQDKPEFISRRQTLVQKL
                     RESRIPVFPTIDRAAKVIYRLQRYRKFLHPET"
     gene            complement(14085..15014)
                     /locus_tag="VEL68_04820"
     CDS             complement(14085..15014)
                     /locus_tag="VEL68_04820"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ35321.1"
                     /translation="MRLKNGPVLFLLAMLLMAWTGCATQSEVRRKQSQAMDLEIKGAD
                     FFQQGQYLKALGYYQRALQAYGSIDHREGVAKSLNSMGSVFLVMGRTPAALEYYQQSL
                     AIYSDLGNDAGMSLNLNNIGSVSLSLGKPEAALDFFQKSLAMEEKRGDIRAQAVRWNN
                     VGLAYRALGQNDRAMECYLRSKTLNEKSENLPGIADNLNNMGAIYESQKDWTRALEAY
                     QGALDSDKKRENSLGISTGLANLGRLYETMGDKEKALDHYLRAWKVNESLGLKERILY
                     DLGKIASLYEALGKPQEAEKFRKRSQELTSSSK"
     gene            complement(15118..15510)
                     /locus_tag="VEL68_04825"
     CDS             complement(15118..15510)
                     /locus_tag="VEL68_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HXZ35322.1"
                     /translation="MIYSDRNASESKLSLAISYLLTAGVLTSLILMAVGILLFYFQFG
                     NLAISEKKVMFLQEKNFFYFLYDLLKGGNSQEKSLWIMTLGIAVLILTPFARVILSVV
                     YFFLEKDFKFSLITLWVLLILTLSLATH"
     gene            complement(15507..16337)
                     /locus_tag="VEL68_04830"
     CDS             complement(15507..16337)
                     /locus_tag="VEL68_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXZ35323.1"
                     /translation="MLSMVSLLLGSIPVGFLGALTGLGGASILIPILVSMGIPVKEAI
                     ACGMASIIATSSGSAVSYVRERIANVRVAMFLEMFTTVGAIVGATITTMISPLFLYFF
                     FAAFLASSFLKSRGTVSQSATSSEWPDGVSRWLNLQGSYFDEAHGKRIDYKVSHPILG
                     GGGMLLAGLAAGMLGIGAGAFKVTVQENILKLPPKVSSTTSNFIIGMTALAGTSVYLY
                     SGFLNLTLMGPMAVGTTIGAFLGGRVLNKLGNRTLRVLFFLIVIFLIVQMIYKGVRSL
                     "
BASE COUNT         3523 a         4672 c         4715 g         3816 t
ORIGIN      
        1 ggccattctg ccctacgacc agtccatgca ccgctttcca gcctatttcc aacagggaaa
       61 tatggaaagc aatgggaaat ccgtcgatcg gaagggcgag agggtatcct accagacagg
      121 tcccatcatc tggggagagc cgggaacgaa cgggcagcac gccttctacc aactggttca
      181 ccaggggacc aaactcattc ccgcagactt tttggcccca acgatcagcc acaacccgat
      241 cggggaccat cattccatcc ttctctccaa cttcttcgct cagaccgaag cgctcatgaa
      301 tgggaagacc aaagaggagg tgatcggaga actaaggcgc gaaggaaagc ccgaagagga
      361 aatccaaaga ctatggccct accgtatctt cgagggcaac cgtcctacca attccgtcct
      421 ctttcagaaa ttaacccccc gagtcctggg aagcctgatc gccatgtatg aacacaaaat
      481 cttcgttcaa gggatcatct ggaacatctt cagcttcgat caatgggggg tggagctggg
      541 caaacagtta gccggcaaaa tccttccgga attggcggag gaccgccctg ttcattcgca
      601 tgattcctca accaacggat tgatcaacac gttcaaggaa atgaggaaag atggcaaggg
      661 gaatgaataa cctttcctcc gcggaaaaac cgaaaggatg gtttccccgc ctcatttccg
      721 gcaaccggac ggcctttgcc cttttgccgg ctctggtgtc ccttgtcctg gcataccggg
      781 cccgtctcac ctacggttta tttactcatc cgattaaacc cttcgatttc tcacccggcg
      841 cccatccggt ccggttcacc cttgggtttg gcgattccga tctgtttttg atcctgtcct
      901 gttttttcct ttttctgctc ctctcccagg ggcgcttcct cctgaagagc cgcaaggtct
      961 tcctcccttt ccaggttgcg gggctggttc tcctccactt gatcctggga agtctcctgt
     1021 tccttcacct gggccatttc cgctttctct tcgacgccca gacggggttc gatttcgggg
     1081 tgatcctaga gtcggtgtac agcgtctcca ccatcggcac cctgaaattg ttggaggtcc
     1141 gggactggtt gttcgtcctg ttccccctgg caatcttctg ggtggtttgg ctggttcctc
     1201 cagcggcgcg agcctgggtg gcgaaggctt tcctcgcggg cgtggttttc tggggggcct
     1261 tctccttgtt gggtcctccg gaaaagaaga ataccgttcc ggcggagctc cgggtgaatc
     1321 ccggaatttt cttcctttcc gacatggccg agcacttggg caggtctccg ataagggacc
     1381 gaacgggcat ccgggttcag gagacagaat ccggattcaa gccggccggg ccattgaatt
     1441 ctcatcccgt acgggcagtg gaagaccttc ctccccaagc caatcatccc tggaatgtgg
     1501 tattcctggt tctggagtcc gtgggaaccc gctacatgca ggaaggctac ggcggaagcc
     1561 cggttcccat gccctttttg aaccggctgg ctggggaggg ctggtttctg aaaaaccatt
     1621 tcactccctc caatgtgagc accaaggcgg atttttccat tttcagcggc ctctacgatt
     1681 tcttccagcg gggaacgctg ggagtgcggc aggatgtcca gatcccatcc ctgtacaatt
     1741 tcctgggccc gaaatacgat tcttttctcg tttctccctc ctcttccgcc tggtatttcc
     1801 ccctcccgtt cttgagaaac ggggggctgc gagaaataca tacctacgaa aatctgaact
     1861 tcaagatccg ggaggaatta agcacgctgg gccgttacat cgcccgcgac gaaattcaga
     1921 ccgtggattt tttcctccag agaatcagca gggcccggga gcccttcctg gggatgtacg
     1981 tgagcttcgt cgcccatttc ccgtatttcg actacggacc tgaataccgc atcgtggaga
     2041 gtgacggaaa gtcgatcagc cgctatatca acaacctgta cctgctcgac cggctggtcc
     2101 agcggatttt tgagcacctg aagaagcggg gccttttgga gagaaccatc ctggtgatcg
     2161 tgggcgatca cggtcaggcc ttcggccagc accatccgga caactacctt cactaccgat
     2221 acagctacaa cgtaaatctg gaggccccgg tgatctttta ccaaccggct ctgttcaagc
     2281 cgaggtcttt caacgtaccc accaaccata cggatattct gcctaccctc ctggacgccc
     2341 tgcggatttc ttacaacccc tccctgctgg atggagagtc cctattccac cctctgaagc
     2401 ggaaatacat tttcttttat gggcacgagg aaagtatttc ttcgttgagc accgatggga
     2461 tcaaagtgca ggtctctctg aagaagaacc gctgctgggc ctttgacctc agggccgacc
     2521 ccgatgaaaa aacccccctc gactgcggtc cttatcagtc ccagttggag gctcttcgca
     2581 actttgtccg cgaccacgat accaacctgg ctcagtacaa tgcctgcaac cgggaaggta
     2641 aagattttca ggggcagaga caccccgccc ttgcggcacc gccgcccggc agagaagaga
     2701 agaaggggaa gggatgaatt ggaaatgggg aagtaaaaga ctgcccgact aggcgacgat
     2761 gcgacctaag tcaatgattt ccatccggtt gttggcctcg gggtgcttta ccgatgggcc
     2821 ttctgtgaga actacgagat ttcctttcac ctcattgtcc tgcagccatt tccgggcaaa
     2881 ggcgttccag tcttcggggt gatccggttc gtgtaccgga taaaccccgt atgagaactg
     2941 gaggcgctgg caggtggact cctgggagct caccgcggtg atccagaccg gagggcgaaa
     3001 ccgggcaata gaccgggcgg tggccccact gtgggtgggg acgaaaacgg cagcgggaga
     3061 aatccgttcc agggtcgtct ccacgctcag ggcgatcagg tcggtcaggc gggtccggtc
     3121 tccccgccca acgccctgca gcgtctcccg aatataatgg gcgggccggt agggttcaat
     3181 ggcggctgcg atcttggcca gcatggccgc cgattcgaca ggatactttc ccatggccga
     3241 ttccccggag agcatgacgc aatccgtccc gtccaggata gcattggcca catcggtggc
     3301 ctcggcccgg gtggggcgac gattgtcggt catggactcg agcatctggg tggctgtaat
     3361 aaccggtttg cccagcaggt tggccttccg catgagctgc ttctggacca gggcaatctg
     3421 ctcgatgggg atctccacgc ccaggtcccc ccgggcgatc atgattccgt ctgcggcgtc
     3481 gaggatttcg tcgatgtggg aaagagcccg agatcgttcg actttggcga tgataaaggg
     3541 ggaaaacccc aaggaggagg cggcctgacg aacggcgttc aggtcttttc ccgattcgac
     3601 gaaagactga ctcactgcgt ccaccccgtt ttccagggcg aattgcagac agcggcggtc
     3661 atgatcggtg aaggcggtga ttcccaaatc aattcctgga aggttcaacc ctttgcgaga
     3721 gcgcaattct cctccgacga tcacccggca taggacctct ctgcctttga tcccaaggac
     3781 ttccagctgg atgaagccat cgttcaggaa aatcatatcc cccgccttga cggactgcgg
     3841 aagggtagaa aagctcaccg aaactcgctg ggaatccccc aagacctcct ctgtggtgag
     3901 agtgaaagtc tcccccggtt ttaattcgat gggttctttg ccgaactggc cgattctgat
     3961 cttgggaccc gggaggtcag ccaaaatggc cagccgataa ccagcttccc gggcggcagc
     4021 cctgaggttt tcgatcaccc gtttatgccc gggaaaatcg ccgtgggaga aattaagacg
     4081 ggccacgttc atccctgccc ggatcatctt tcccagcatt tccggagact cggaggccgg
     4141 accgatggtg cagacaatct tggtcttatg cggtgggagt ttcatttctt ctccaatcta
     4201 accaaaagaa attcaaagcc attgaaaaag gcgcagagaa tcttcttccc gcaggccgtc
     4261 ggtcatgctt ctgcggcgac tttccaggat gcttgaatgg tcgttccttt ccctttcccg
     4321 gactggacag agaaaaaccc cccggagagc tcggtccttt cgcgcatact ggagaggccg
     4381 aatccgtact tttcactctt tcgggagata atccggtcca gttcgactcc gactccatta
     4441 tcctggatca ccagttcgag acagtcgttg acctttcgca aggagaggag aatctttgtt
     4501 gcctggctgt gtttggcaac gttattcatg gcttcctgca ggacccgata aatgaccgtc
     4561 ttgagaggcg gggggacttc atcctcttcc acctccagct gcctctggac ccggatggac
     4621 ccataaattc cctggtactc gcggcaaaac caggagatgg tggccaggat tcccaggtcg
     4681 tcgaggatcg ggggacgaag gtcggactgg attcgtcgca cttcctcgat ggcattttgg
     4741 atcatgggaa tcatgctttc caggtcccga aggttgccgg gagaggagtt cttctccatc
     4801 cagtggaggc gattttccag caggaacttc atggccgaga gggactgccc gatcccgtcg
     4861 tgaagctccc gggcgattcg tttccgctct tcttcctgca ctgaaagaag ctggacggaa
     4921 agacgccgaa gctcttcctg cgaacgctgg agcatctgct cggcctgctt tcgctcggtg
     4981 atgtcctgaa cggttccgat catgcggatc ggacgccctc catcatcgaa agtgacctcc
     5041 ccccgctcat ggacgattcg ttccgagtga tccggcctta caatccggtg ttcgatgccg
     5101 taaaggatgg acgggtcggc cagactccgg gtgacagcct ctttgaccgc ttcccggtca
     5161 ccggcgtgca cgaattccag gaaagcgttg taggtggccc cgaactcctg cggatggagg
     5221 ccgaagatcc ggtaaacttc gtcggaccat gccagttcgt tggtttcgat attccagtgc
     5281 cagttaccca tgtgggcaat tctctgcgct tccgcgaggc tctcgtcctt tttccgggcc
     5341 gcctcttcgg ccattctccg ttcggtcacg tcgcggttgc tgactcggta gcccaggaat
     5401 tccccctgag ggcttcttac cgactggcag gcatgctcga tccagcggac ctgaccgtcc
     5461 ggcttgcgga tgcggaactg aatttcgtgg gcgaggaccc ctcgccggct ttcccggaag
     5521 tgctggtccc aggctccccg gtcgatggga aggatgatct ctctgattaa agaggggcga
     5581 ttaacaaact catcgggcga atacccggtg attcttctgc aggaagggga catgtaccgt
     5641 atggtgccat cggggttgga ccagtattcc cagtcgtaag ggaattcggc caccatccgg
     5701 tattccagct cggcttggcg gagcgcctcc tccgattgct tgcgggccag ggcgctggcg
     5761 aaaatctctc cgaccaggcg gactcgctga atcagccctc ccggccagtt tcgttcggcg
     5821 cggaccgctc caaaggttac ggcaccaaag acgtttttcc ctacggccag aggaatcacc
     5881 agggaggata aattccggaa tttcttcaat tcctcccgat ccttcgccgc ttctggtggg
     5941 agatcgtcga cgcgcgggat gaccagcatc tcgcccttct gcagtttttt gctgagccag
     6001 ggaaacttct gatcctccag gacgggcgct gattcgggaa ttccctccat tccccagaag
     6061 tatttccgaa agatcctggt tctgtccccc gaaaagatat agacgcttcc ccggtcaact
     6121 cccaggaatt tgaccacccg acgcagcccc agctcgatct cccgttcgat ctgatcagcc
     6181 agaagatgga ccaggcgggt ggagatttcc gaaagcacct tctcgaaacg aatgatttct
     6241 tcaaactgac gcgctttttc gcggaattct tccaccttgc gctgcaaccg ggtgttcttc
     6301 cgggtcagct cctggatttt ttcctgcgga gtctttcctg ttcttggacg tgattttgga
     6361 gaaggctttt tcgttttcac catacaatct tcgatggttc aaaaatcatg ggactcttat
     6421 tttgccggcg ttctccgcat ctctctacga gagatcatga attttggttc ctccgcatcc
     6481 gatcaatacc cgatggccgc cccatcgcag cgtgggtccg agccccccat gcgcaaccca
     6541 ttctccggat ggaccatgat cccatgggcg cttcccatgg attggtcgat tccatcgacg
     6601 atttggattt ggtggccccg ttttctcaac tcctcgacca cgggcgcagg aaaccgtccc
     6661 tccatcatga gaagcccttc cgaatgatgg agaagataac ggcctgcaac ccagcgcggc
     6721 gcctcgatgg ccgcctggat atccaggccg aagtcgaaaa ccccagtcat gacctggatg
     6781 tgggtctggg gctgcccgtc ggctccgctg gtcccgaaca cgaggagggg tttcccatcc
     6841 cgaaaggtca tggaagccat gagggtatgg aaggtgcgtt tatggggctg caagcagttt
     6901 acatgatccg ggttcaagga gaaataagcg ccccggttct gaagcaggat cccggtgttc
     6961 ccggccacga ccgccgagcc gaaggaatag tagagggact ggatcaggga cacggcgttt
     7021 cccgcctcat ccacggtgca cagatagatc gtatcccgcc cgaaaatccc tggagagatc
     7081 gcctgagggt ccacggcccg gtctttctgg atgagggccc ggcgcttgtt ggcatagtcc
     7141 ttagagagga ggtcgtccat gggaatctcg accttctcag ggtcggagat gtaccggttc
     7201 cggtcggcga aggccacttt cttggcttcc accatgaagt gaagatggtc ggaggactga
     7261 tagcccatgg aggccaggct gtagccctcg aggaggttca ggatgaggag ggcggtcagg
     7321 ccctgagagt tgggggcggt ttcgtagacc gtgtaacccc ggtactgggt ggacacaggc
     7381 tttccccagt cggaccgatg ttccagaaaa tcccgctcgg tcaagaggcc gccgttttcc
     7441 cgggaaaagc ggacgatttc ccgaccgatt tccccttgat aaaacacgtc ccttccttct
     7501 cgggcgatct tttccagaga agaagccaga ccgggattgg tcaagacatc cccaggccgc
     7561 ggagcctttc ccccggggag aaaaaggcct ttggaagtgg agtaggaaga aagttcgctg
     7621 acggcctcgt gggtcttgaa gcttaggacg tggctgatgg gataccccct ccgggagtaa
     7681 gcgattgctg tttcgaggag cgcggccatg ggcagtcttc cgtatcgccg gtgggcctca
     7741 aaccagccgt ccacagcccc ggggacggtc acggtcaggg gaccccggac gggcatggaa
     7801 ttcatcccgg cttttttgta agtctcgatc gtagccgcat aggcggaacg gccgctggcg
     7861 ttcagaaagg ccaaatcctt tttggcggcg tcgtaaacga gccagaaggc gtccccgccg
     7921 attccgcaca tgtgggggta aacgacgtta aggacagaat tagtggcgat ggccgcgtcc
     7981 accgccgtac cccccttctt tagaatatcc acaccggcct gagaggcgag atagtgggga
     8041 gaggccacca ttccgtgagg ggccatgatc aaaggacgat acctttgtgg aaatgtttca
     8101 ggggttttga acattcggag ctcctctcta aaaaaatgcc tcaaaggcaa tgataaaatt
     8161 cgaaatttga atatcggaat cggaaacaaa ttcgaattac agaaattcaa attaaggcaa
     8221 catcggtttg aaaatttgaa attcgaattt tgttattctt tcggatttcg tgcttcgaat
     8281 ttcggatttc ctcccggctg aatccttctt ccgctaccct gtcccttctc cctcctctcc
     8341 tgaagaggcg gcgggtgaag acggatgaaa gaactcaaag accgtctgcg cgtccctgtg
     8401 ggtcatggga cctacctgac tgagcctgtc cggagaagcc tcctgaatct tctgcgggct
     8461 cccgaattcc ttgagcaagg ctttcttctt tacctctccg atccccggaa tatcgtccag
     8521 gagcgtccgc aacccgtgct tgccgcgcaa cttcttatgg taccgaatgg caaaccggtg
     8581 ggcctcatcc ctgattctct ggagatagcg caatgaagcc gaccggccaa ggagcacagg
     8641 gtcttttacg ttcggaaggt agaccttgtc ggcggtcttc tccgcgatct tcctcagact
     8701 gggccccttg tccttggcca gccctacggc ggaaatatcc tgcaggccga gctcggccag
     8761 gactgccagg gccacattga gttgcccttt tcctccgtca acgatgagga ggttcggctt
     8821 ttcccctcca ggctccagtt taccgtaccg gcgcctcaaa atctcataca tcatagcata
     8881 atcatccgga aaagaggggg cgtgaacctt gaaatggcga tagtgggaag gttccgggcg
     8941 cccatccaga aaactcacca tggaacctac agctagggtc ccgaagaggt tggagatgtc
     9001 gaagcattcc gcccggtgag gaacggtttt cagcctcaat ttctctttca gctcctgaag
     9061 aatcagagcc agagtttcgg tatcgccgac ccttttctgg aaggagtttt cagcgttttt
     9121 cccggccatc tccaccaggc tttttttctc tccccgctgg ggggcgtgaa cttctaccct
     9181 gacccccttc ttttcggaaa gccactcggc cagtaacctt tcatcttcga tcggcagagg
     9241 caggaggatt tcgtggggaa tggagggctc ctcggcatag tactgacgga ggaaagcgga
     9301 caaggcctcc tcctccggca ggttgagacg ggggaagtga aaggccttgt tccccaccag
     9361 aattccccgc cggaagaaaa ggacctggaa ctcccaggcg tttccccggc ggtggaaggc
     9421 gaagacatcc tggtcgttgg gggtctggga gacggctttc tgcttttcca gggtttgttc
     9481 aatagcggcg atctggtccc ggaggcgtgc cgcctcttcg aagttaaggg cttccgatgc
     9541 ctcagtcatc ctttcctgaa gaagcttgat caactgggag ttcttcccct cgaggaagag
     9601 aataacttcc tgaaccaggt ccgcgtactt ctcggaggaa accatgccgc agcagggtcc
     9661 caggcatttt ttaatttgga agttcagaca gggacgggag cggttccgga aattggcgtc
     9721 gttgcaggtg cgcaggggga agagatgacg caggacccgg agggtctctt tcaccgccgc
     9781 gctagaagcg taggggccga agtagcgggc cccgtcccgc ttgattttgc ggaccagggt
     9841 gagtttggga aattcctcct caaggttgaa cctgaggctg aagtaggtct tgtcgtcttt
     9901 taaatcaata ttgtggcgtg gtttgtgctt cttgatcagg gtgttctcga ggatcagggc
     9961 ctctttctcc gtgtccgtga ggaaggtctc cacctggcgg actttgggca ttaagaagcg
    10021 gagggagagg cgggcatccc cggattttcc gaagtaggtt ctcacccgac tgcgcaggtt
    10081 cttggccttc ccgatataca acaccgttcc ggcctgatcc ttcatcagat agacaccggg
    10141 gctcgtaggg agagactgga tttggtcttc caggttcata ttagatactg gatactggat
    10201 actcgatgct ggataaggga ttaaaaaatg atatatccga tatccagcat ccggaatcca
    10261 gtatcgagct ttgacttacg cttctgctgc ccttccttcc ctggtatcca gctccttttt
    10321 ctgcagcgcc tggatccggt cccgcaactc ggcggctctt tcgaaatcga gccgggaggc
    10381 ggcttcgcgc atctccttct tcaggcggac gatgagtttg gggacctcga agagggaaac
    10441 gtattcctcg ggcttctctg cggccatggg cacagtgacg tagtccgctt cgtaaatgga
    10501 ctggaggatg tcagggatgg ccttgcggat cgattcggga gtaatccggt tcttgcgatt
    10561 gtaatcttcc tggacctttc gccgccggtc ggtctcccgg atggccttct tcatggattc
    10621 cgtttcctgg tcggcgtaga ggatcacctg cccgcccacg ttccgggccg cacgtccgaa
    10681 ggtctggatc agggaggtcc cggagcgtag gaacccttcc ttgtccgcat cgaggatggc
    10741 caccaaggag acttcgggga ggtccagccc ctcccgcagc aggttgatgc cgatgaggac
    10801 atcgaacttc cccaagcgca ggtcgcggag gatctccgtc cgctccaggg cctggatgtc
    10861 cgagtgcagg tacttgacct tgatccccag gtcagcatag taggaagtca ggtcttcggc
    10921 catgcgcttg gtcagcgtgg tgaccaggac gcgttccccc cgggcgaccc gggcctggat
    10981 ttcattcagg aggtcatcca cctggtatcg ggcgggttta accgttacct gcgggtcgat
    11041 caggcccgta gggcggatga tttgttccac cacccgaccc cgagacttct tcatttcgta
    11101 agcggcgggg gtggcagaaa cgtagaccat ctggggcacc cgggcttcga actcctcgaa
    11161 ggagaggggc cggttgtcca gggccgaggg gaggcggaag ccgaactccg ccagggtttc
    11221 tttccgcgtc cggtcgcccc agtacattcc attcagctga ggaatggtga tatggctctc
    11281 atcgataaaa taaaggaggt ctttcggaaa ataatccagc aaggtatagg gaggctcccc
    11341 tggttttcgg ccgtccaggt ggcgcgaata gttttcgatg ccggtgcagt atcccatctc
    11401 ctggagcatc tcgatgtcgt agttggttcg ctgttccagg cgctgggctt ccagtaattt
    11461 tttctgggat tcgaaaaaag cgagccgctc caccagctct tcccggatag attgaatggc
    11521 tcggcgcagg cggtcctggg gagtcacgta atggctgccg gggaagatgg tgatctgctt
    11581 caacttccgc aggacctttc cccgtaacgg gtcgatttcc gagatagagt ccaccaggtc
    11641 tccgaagaac tcgacccgga tggccgtgtt ttcctcataa gccgggaaga cctccagaac
    11701 atctccccga acgcggaagg tcccccggtg aaaatcatag tcgtttcgtt catactggat
    11761 ctccaccaga cgggcaagga cctcatcccg ggaggtctcc ttctggtctt ccataaggac
    11821 gatcatcccc tggtaggttt ctggcgaccc cagaccgtaa atgcaggaaa ccgaggctac
    11881 cacgatcaca tcccggcggg tgaggaccga atgggtggct gaatgacgca tcttgtcgat
    11941 caggtcgttg atggaggcgt ccttttcaat gtacgtgtcg gtggtgggaa tgtaggcctc
    12001 cggctggtag taatcatagt agctgatgaa gtactccacg gcgttgtccg ggaagaggct
    12061 gcggaactcc tggaagagct gggccgccag agtcttgttg ggggcgatga ccagggtcgg
    12121 cttctgcacc cgttcgatca cccgagccat ggtaaaagtc ttccccgacc cggtgactcc
    12181 gaggagaacc tggtgggcga ttcccttttt taccccttgt accagctgtt caatggcctg
    12241 gggctgatcc ccctggggtt tgaatggaga aacaattttg aagtcgggca tagaaactgg
    12301 tttctggata ctggatgctg ggttccggcc ttgagacgcg ggaccttctc tcccagccgt
    12361 aagggaggtt cgggaacccc cccacatttt cattttaatc catccaaggc tgccggggaa
    12421 gtctatgatc ggttgtatgc ttggagtggt gtaggggtga ccgcccctgt cgaaaggcgt
    12481 agatcaaggg gttgtatggg cgaaccatga atggccctac gtttccggat gcaaaaattt
    12541 tcggtacctt tgcagccggt aaataacctt ggcggcgcgg tcgatggtgg ggaagacagg
    12601 gatcctgctc tcacggagct tttgaacgag agtctggcgc ctggagatga actccggttt
    12661 atcctgctcc agggggggga tgactataac catgggtttc tgcagctccc ggttgacctt
    12721 ggccatttca gcagctatat tagccggcca atccaggtgg ggagtgaatt gctggaaata
    12781 ctcggtgatc tggtagagaa gaagaacgtc aatattcggg tccgtagcgg cgagccggat
    12841 cgattggctg taaacctggg ggaagaagcc catgaatcca aggtccaccg ggtttttaca
    12901 gctcgtattc gcctggggaa tattcttggc gattttctct tgagtctctt ttcccaggat
    12961 gggaatctcc agggaattcc gggcgcagat gtccgtcatc tccacggaac tccctccccc
    13021 cacgactaca atccccaccc gggggcttcg gggcaaatcc agggttaggc aggaaaatgt
    13081 ggcgtcggcc atctcttcca ggtcctccac gagaatgact cccagctggt gggcggcttt
    13141 ctgccagacc ccggagtggg acgccattgc gccggtatgg ctgaaggccg cgcgtgcccc
    13201 agcctcggtg gctcctcctt tccagacgat caacggcttc ttgtgggcca cctcgcgcag
    13261 cgaccgggca aattcctctc cccccctggc ctgctccagg taggcgcaga taaccctgat
    13321 ctgtgggtct cccgcatagt agcggaggac ctcttggatc gtcaggtcac attggttccc
    13381 gagggagaga attttgttga agtgaattcc cctggaactg gccaaggttt tgtaagtgat
    13441 gctgtggcct ccggactggg agacgaaccc tacgttcccc ggctccgcgg ccttttcttc
    13501 tatggcaaag gtgacgccgc tttccgggca ataggggccg atgcagttgg gaccgacgag
    13561 ccgggtaggt ccctggcaga ctagggcttt gatttcttcc tccagctccg ggtggccgcc
    13621 ctccgtgaat cccgaggaaa agatcactgc gaaccggact cccttacgca cgcaatcggc
    13681 gacgatgggc ggaaccttag gagcgcgcag ggtgatgatg gccaggtcta tgggttccgg
    13741 gatttcctgg aggcgggaaa aagccttgcg gccgccaatc tcgggagagt tgggattcac
    13801 cggatagagc cttccgggaa atccagcctt caaaaaagcc tggaagaaag gaaaccggtc
    13861 tcccttcact tccggagagg cgcccacgac cgcgacgctg tccgggtaga agagtttttc
    13921 cagggaaggg ttcttccgtt cccctgatgg aaatatcttt gccatggtcc ttgcgcagaa
    13981 tcgccccttc acccagccct ctcccccgaa ttcgggggag aggagaaagg gtaggggaaa
    14041 atcatgaata aggctcgcgg gtgtcgcaag cctgccttga tgaactactt ggaagaggaa
    14101 gtcagttcct gggatctctt ccggaatttt tcagcttctt ggggttttcc cagggcttcg
    14161 taaagggaag cgatttttcc gaggtcgtaa agaatgcgct ccttcaggcc gagggattca
    14221 ttgaccttcc aggcgcgcag gtagtgatcc agggcttttt ccttgtctcc catggtttca
    14281 tacagccttc ccaggttggc caggccagtg gagattccga gggagttttc cctttttttg
    14341 tccgaatcca gggcgccttg atacgcctcg agggctcggg tccagtcttt ctgagactcg
    14401 taaatcgctc ccatgttgtt caggttgtcc gcgattcccg ggagattttc acttttctca
    14461 ttcagtgttt tcgatcgaag atagcactcc atggcccggt cattctggcc cagagcgcgg
    14521 taagccaggc ccacgttgtt ccaccggacg gcctgcgccc ttatatcccc ccgcttttcc
    14581 tccatggcca gggatttctg gaagaaatcc agggcggcct ccggctttcc cagggaaagg
    14641 ctgacgcttc cgatattgtt caggttcaga gacatccccg catcgttgcc caagtcggaa
    14701 tagatggcca gcgactgctg gtaatattcg agggccgcgg gagtccgacc catgaccaga
    14761 aatacggacc ccatgctgtt caggcttttg gccacccctt cccgatgatc gatggatccg
    14821 taggcctgca gggctcgctg gtaatacccc aaagccttca ggtactggcc ctgctggaaa
    14881 aagtcggccc ccttgatctc caaatccatg gcctgggact gcttcctccg gacctccgat
    14941 tgggtcgcac accccgtcca ggccatcagg agcattgcca aaaggaacaa gacaggacca
    15001 ttcttgaggc gcatctttcc tattcccccc aacgcttatg cagtgtatca aggtgacgag
    15061 aaattagcaa gaggaaggaa tgctttggga acccatgaag aaaatgggag aatcggttca
    15121 atgggtggcc aagctcaggg tcagaatcag gaggacccaa agagtaataa gagagaattt
    15181 gaaatctttt tccaaaaaga aatacaccac agaaagaatg acccgggcaa agggagtaag
    15241 aatgagcacg gcgatgccca gggtcatgat ccagagcgat ttttcctgag aattccctcc
    15301 ctttaggagg tcgtagagga aatagaagaa attcttttct tgaaggaaca tgaccttctt
    15361 ttcggagatg gccaaattcc caaattggaa atagaagaga agaattccga ctgccattag
    15421 aatcaggctg gtcaggactc cggccgtaag caggtaacta atggccaacg acaatttaga
    15481 ctcgctcgca ttccgatcag aatagatcaa agggatctca cccccttgta aatcatctga
    15541 acaatcaaaa agatgacaat caaaaagaag aggacccgga gggtccgatt gcccagtttg
    15601 ttcagcaccc ggcccccgag gaatgccccg atcgtggtcc ccacggccat aggccccatg
    15661 agggtgaggt ttaaaaagcc ggagtaaaga tacacgctgg ttccggctaa ggccgtcatg
    15721 ccgatgatga aattgctggt ggtactcgag accttgggag gcaattttaa aatattctcc
    15781 tgaacggtca ctttgaacgc ccccgctccg attcccagca ttccggcggc caatcccgcc
    15841 aaaagcatcc cccctccccc tagaatggga tggctcactt tatagtcaat tctttttcca
    15901 tgggcttcat caaaataact tccttggagg ttcagccagc gagatacccc atcaggccat
    15961 tcggaggagg tcgcggactg ggagaccgtc cctctgctct ttaaaaaaga actggccagg
    16021 aaggcagcga agaagaaata gagaaaaagg ggagaaatca ttgttgtgat cgtagctcct
    16081 acgatggccc cgaccgtggt gaacatctcc aggaacatgg ccacccgaac attcgcaatc
    16141 ctctcgcgca cataagacac ggccgagccg ctggaggtgg caatgatgct cgccatcccg
    16201 caggcgatgg cttccttaac cgggattccc atggaaacca ggataggaat caggatactt
    16261 gcccccccaa ggccggtaag ggctcccaga aacccaacgg gaatagaccc taaaagcagt
    16321 gaaaccatgc ttagcataga ggaaatcttg ttgcttaata agaacggctc ttccaaccga
    16381 gtaggtcgaa aggtagcaga gatgttctca gattcatctt agcagggata ttttggagat
    16441 acaaggaggg ccaatggatt cagggagttg ggatatataa tttccttggg aaaaatgaaa
    16501 gggaggccga atcgatcctc ggccccccaa gttttagccc atcccgtccg gatcatttcg
    16561 acaaagcggc gaagaaagtc ccctcattgc gcctcatgag gagcaggagg gaatcggtat
    16621 cttttacttt ggaaatcact tcgaccgcct gtttcgttga atttaccgcc tgacgattca
    16681 cgctcaaaag aatgtctccc ggatgtacac ccgccaggtc cgccgg
//
LOCUS       DASPRR010000131         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1321195, whole genome shotgun sequence.
ACCESSION   DASPRR010000131 DASPRR010000000
VERSION     DASPRR010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321195"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VEL68_04835"
     CDS             complement(<1..642)
                     /locus_tag="VEL68_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HXZ35324.1"
                     /translation="MASMIDSPLFGGLFTTEEMRKVWSDEATIQKWLDVEAALARAEA
                     KLGIIPPRHAREITRKARVELIDMEEMKRQLFHTHHPIMPLIRCFQKACSPAAGEFIH
                     WGATTQDIMDTGAVLQLRDANDLVIRDLKETYRLLSRVAKKYKNTVQAGRTHGQHALP
                     ITFGYKVAVWASEIKRHLQRIRQLSPRVFQGQFAGAVGTLASIGAPGFKLQKLM"
     gene            complement(662..2089)
                     /locus_tag="VEL68_04840"
     CDS             complement(662..2089)
                     /locus_tag="VEL68_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HXZ35325.1"
                     /translation="MASEKPSLPQRRIASALIPILVLAVIWFSPAPSGLALPAWRMFA
                     IFAATILGILLQPLPSGAIMLLGVAFPIFTGVLSEAKALSGFANGTVWLIFCAYILSI
                     GFVKTGLGKRIAYKLISWLGGSSLGIAYALGVSDLIMAPAMPSVTARAGGVIFPIVRS
                     VNAAMGSAPGPTGKKIGNFLIMTCFLYATVTGAMFLTGMAANPLCAELAKKTLKLDIT
                     WGGWALAALVPGLISFLLLPLVLYWLIRPEMKRTAEAQRMGEKALEEMGPMTAKEWAL
                     TVIFILALAGWATSFYTKMSATTVALGGVGLLFLSGVVGWKDVLREHAAWDTLIWFGA
                     IISLANGLSDLGFIKWLTAQLSGAFAGWSWLATFVALGLIYIYIHYAFATASGHTAAV
                     YAPFIAAAVAAGAPPMMVAICFGIFTNLMWGLTEYGGGPGPIYFSQGYYERPAFYRLN
                     LIVTTINVAIMFIVGIPWWKFINLY"
     gene            2406..>3773
                     /locus_tag="VEL68_04845"
     CDS             2406..>3773
                     /locus_tag="VEL68_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXZ35326.1"
                     /translation="MEVRELQADILIIGGGSGGCLAANRALEINPGLKVAIFEKGDIK
                     YSGSIARGMDALNIVAIPNFTTPELYVESSTLQCAGILDAPPSHEMARRSFGLLQKLE
                     GWGVYFPQDEKGNYRTLQYHPVGNFLTAMEEPDLKVIIARKAEEKGSLVVNRTMGLKL
                     LLDGGRVAGAVGIHVRTGDLVVCRAGAVILSNGGTARFGLPNSGYLYGTFDYPGNTGD
                     GYVMGFHAGASLTGMEYTRRVNLIKDVNIPLLAITITRGGKMMDIFDEIVMEWSCHDQ
                     TKANQAVAAGKGPLRIRVSHLPEEVIREIESILFSTERPVQERFFKGRGVDFRKGDIE
                     LWPTENQLCGGHGMSGLVVNEKAETAVPGLYAAGDVACVSKGHLTGAFVFGEIAAEQA
                     VRFIASQPPPKFDPGQIAGMQRKIEKRGSRKGNGVDVRDLEFKVRRLINDYVISPKNA
                     YKLSRW"
BASE COUNT          837 a         1099 c         1130 g          707 t
ORIGIN      
        1 catgagcttc tgcagcttga accccggggc gccgatggag gccagcgttc ccactgcccc
       61 ggcgaattgg ccctggaata cgcgggggga gagttgcctg atccgctgca ggtgccgttt
      121 gatctccgag gcccacaccg ccaccttgta cccgaaggtg atgggcagcg cgtgctggcc
      181 gtgggtccgc ccagcctgga ccgtattctt atattttttg gcaacccggg ataacaagcg
      241 ataggtttct ttcaggtccc ggatcaccag atcattggcg tcccgcagct gaagcacggc
      301 gccggtgtcc atgatgtcct gggtggtggc tccccagtgg atgaactccc cggccgcggg
      361 agaacaggcc ttctggaagc agcggatcag gggcataatg ggatggtggg tatggaagag
      421 ctgacgcttc atctcctcca tgtcgatcag ctccaccctg gcctttcggg tgatctcccg
      481 ggcgtgtctt ggggggatga tccccagctt ggcctctgcc cgggccaggg ccgcctccac
      541 gtccaaccat ttctggatgg tggcttcgtc actccagacc ttgcgcatct cttccgtggt
      601 gaacaggccc ccgaagaggg ggctatcgat catactggcc atattcattc ctcctttgcg
      661 gtcagtaaag gtttataaat ttccaccagg ggatccccac aatgaacatg atcgccacgt
      721 ttatggtggt tacgatcagg ttcaaacgat agaaagccgg ccgctcataa tatccctggc
      781 tgaaatagat gggcccgggc ccgccgccgt attccgtcag gccccacatc aaattcgtga
      841 agatgccgaa gcagatggcc accatcatgg ggggagctcc ggcagccacg gccgcggcaa
      901 tgaagggggc atagaccgcc gccgtatgcc cgctggcagt tgcaaaggca tagtgaatgt
      961 agatgtagat caaaccgaga gccacaaaag tggccagcca ggaccatccg gcaaaagccc
     1021 cgctgagctg ggcggtcagc cacttgatga atcccaggtc cgaaagtccg ttcgccaggc
     1081 tgatgatggc gccgaaccag atcagcgtat cccaggcggc atgctcgcgg agcacatcct
     1141 tccaacctac caccccggaa aggaacaaca accccactcc tccaagagcc accgtagtgg
     1201 cactcatctt ggtgtaaaaa ctcgtggccc agccggccag ggctaggatg aagataaccg
     1261 tcagggccca ttcctttgcg gtcatgggcc ccatctcctc cagggccttt tcgcccatcc
     1321 tttgggcctc tgctgtacgc ttcatctccg gcctgatcag ccagtagaga accaggggaa
     1381 gaaggaggaa gctgatcaac cccgggacta acgccgccag ggcccaacct ccccaggtga
     1441 tatccagctt caaggtcttt ttggccagct cggcgcacag gggattggcg gccatgcccg
     1501 tcaggaacat cgccccggta accgtggcgt acaaaaagca ggtcatgatg agaaaattcc
     1561 cgatcttctt gcccgtgggc cccggcgcag accccatggc cgcgttgacc gaccggacaa
     1621 tgggaaagat gactccgccg gcgcgggcgg tcacggacgg catggctggg gccatgatta
     1681 aatcactgac ccccagggca taagcgatgc ccagactgct cccgcccagc caggagatca
     1741 atttgtaggc gatcctcttc cccagccccg tcttcacgaa tccgatggag agaatgtaag
     1801 cgcagaatat caaccagacc gtgccgttgg caaagccgga aagggctttg gcttcgctca
     1861 aaaccccggt aaaaatggga aaggctaccc ccaggagcat gatggcaccg gaaggcaggg
     1921 gttgcaggag aatccccagg atggtcgccg cgaaaatggc gaacatcctc caggccggaa
     1981 gagccaagcc agaaggtgca ggggagaacc agatcaccgc aagcaccagg atgggaatca
     2041 gagcggaggc gatcctcctt tggggaaggg acggtttttc gctggccatt tatcttcctc
     2101 ctttgtcttg tcttctttga cccctcgccg ttcttaaccc catcatttcc atattccaga
     2161 aaagaaaaaa attcaacaga atttcttaga aattatggac cctgaaggaa cggcctggag
     2221 gatggaggaa ggaaagtact ttccggcata cttccctttt caattttgga gaaagaggat
     2281 atgctgaatc gaaagtgccg gggtggaccc ttccggggag aaggcgtgat ttttttgata
     2341 ccgaatgttg aactcaggcg cgaatccggc aaaagaatag aaaccgggca agaaaaggaa
     2401 agaccatgga agtgagggaa ctgcaggccg atatcttgat tatcggcgga gggagcggcg
     2461 gttgcctggc ggcgaaccga gccctggaga tcaaccccgg cctcaaggtg gcgatctttg
     2521 aaaagggaga catcaagtat agtggctcca tcgcccgggg aatggatgcg ctcaacatcg
     2581 tggccatccc caatttcacc acccccgaac tctacgtgga gtccagcacc ctccagtgcg
     2641 caggcatcct cgacgcgccg ccctctcacg aaatggcccg ccgttccttc gggctcctgc
     2701 agaagctgga gggatggggg gtctatttcc cccaggatga aaagggaaac taccgcaccc
     2761 tccaatacca ccccgtcggg aattttctca ccgccatgga ggagccggac ctgaaggtga
     2821 tcatcgcccg gaaggctgag gagaaggggt ccctggtggt taaccggacc atggggctga
     2881 aactcctcct ggatggtgga agagtggccg gcgccgtagg gatccacgtg cggaccgggg
     2941 acctggtcgt ctgccgggca ggggcggtga tcctttccaa cgggggaacg gcccgcttcg
     3001 gccttcccaa ttccggatac ctctacggga ccttcgatta ccccggcaat accggggacg
     3061 gctatgtcat ggggtttcac gcgggagcga gtctcaccgg catggaatac acgcgccggg
     3121 tgaatctcat caaagatgtc aatattcccc tcctggccat caccatcacc cggggcggga
     3181 agatgatgga catcttcgac gagatcgtca tggagtggag ctgtcacgat cagaccaagg
     3241 ccaaccaggc ggtggcggcc ggtaaggggc cgctgcggat ccgcgtgagc cacctcccgg
     3301 aagaggtcat ccgggagatc gaatccattc ttttcagcac ggaacggccc gtgcaggagc
     3361 gcttcttcaa aggacggggg gttgatttcc gcaaagggga catcgaactc tggcccacgg
     3421 agaaccagct ctgcggaggc cacgggatgt ccggtctggt ggtcaacgaa aaggccgaga
     3481 ccgcggttcc cggcctgtat gccgcgggag atgtggcctg cgtttccaaa gggcatttga
     3541 ccggagcctt tgttttcggt gaaatcgccg ccgagcaggc ggtccgcttt atcgcctccc
     3601 agccgccccc taaattcgac ccgggtcaga ttgccggcat gcagaggaag atcgaaaagc
     3661 ggggaagccg gaaagggaac ggcgtggacg tgcgcgacct ggaattcaag gtgcgacggc
     3721 tgatcaacga ctacgtcatt tctcccaaga atgcgtacaa gctcagccgc tgg
//
LOCUS       DASPRR010000132         9676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_519425, whole genome shotgun sequence.
ACCESSION   DASPRR010000132 DASPRR010000000
VERSION     DASPRR010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519425"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1597
                     /locus_tag="VEL68_04850"
     CDS             485..1597
                     /locus_tag="VEL68_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35327.1"
                     /translation="MTLAIIYREELKEYDFGPGHPFRGDRYEIFPKFLQEKLPAKNHY
                     QFLKAEWASDEDLLLICRREYIDFSATYYRSANLGQEYGSLFLQYHSGDNRPHGRPGK
                     LEEAARLIVGQAKKACDLVEKGKFPKAVCLGGGMHHAKPNCGEGFCVYNDVAFAGKYL
                     LKKYGLQRILILDTDAHTGNGTAEYFYEDPRVLFIDIHQDPHTLYPGTGFTHQIGEKE
                     GKGYTINLPMPPYAGDECYRLAFEKVILPVTSEFKPQIILRNGGSDPHYSDNLTNLGL
                     TIQGFRMIGEKVREMAQICGGKVVDMIGSGYNQDVLPYAWFALISGLANFSVPVEEPV
                     HVPAPRKGDPSLNETKEMIQETKKVLKDYWTCLAGL"
     gene            complement(1672..2430)
                     /locus_tag="VEL68_04855"
     CDS             complement(1672..2430)
                     /locus_tag="VEL68_04855"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXZ35328.1"
                     /translation="MQINLTGKVAIVTGAGRGIGKAIAKALAAAKASVVVNDVDWNAA
                     TQVSREIESSGGRALSAKADVRIRSEIEQMVQNTIRELGRVQVLINNAGVILRKPAEE
                     ISEEEWDKVIDINLKGTFLCAQEVAKAMIREGKGGKIINLASIMGEVALPPRAAYCAS
                     KGGIIALTKDLAAEWAKHGITVNTLAPGWTVTEMTESYFSQEPVRQFLLERIPLNRLG
                     KPEDIANLAVFMASEYSDYITGQAIYVDGGWTIL"
     gene            2754..3281
                     /locus_tag="VEL68_04860"
     CDS             2754..3281
                     /locus_tag="VEL68_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXZ35329.1"
                     /translation="MRFAKFVVLAMLLSFSYGCGGGSGSDSSSSGGPGGGGAGTGGGG
                     GTTGSSHPAAAEDCVETINQYRASIGLAPYARLTDAEACADDEARRDAEANQPHGSFG
                     SCSERAQNTCPGWPGTPETMIGSCLAAMWAEGPGSDFSTHGHYINMSSTSYTKVACGF
                     YVTSSGRVWTVQDFR"
     gene            complement(3274..5235)
                     /locus_tag="VEL68_04865"
     CDS             complement(3274..5235)
                     /locus_tag="VEL68_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HXZ35330.1"
                     /translation="MKLLRISISLILLFLSADLFAGQKEITIIHTNDLHSHILGFSPN
                     LDYRPDMTGGDETKGGLARVATGIKREKEKRDHPVLVLDGGDFLMGSLFHMLAREEAM
                     ELRLMKDMGYDAMTLGNHEFDLKPKGLARILHSAAAKGGMPPIVFSNAVFSKESDRDD
                     ALEEAFNQGLVKPYMVLQKGGMKIGIFGIMGKDAAEKSPFASPVTFRDPIETSREMVK
                     ALREKEKVDMVICLSHSGVREAKSISEDEALAQKVPGIDVIVGGHSHTRLEKPILINS
                     TLILQASAYGKCVGVLDLLWESGKVRQKTYRLIDIDSSIPGDMALQKKINSFLGTIDR
                     DVLQKVNLTSKKVIGQSAFDLTIKEEESNLGNLIADAIRWWVNKHDSDPGDPRTKVAV
                     AIESNGLIRDNLVRGKTGALAVTDVFAAIPLGIGMDDTMGYPLISCYFYASEIKKALE
                     VLTSVYPRKGSSYFLQVSGLKFTYNPRRVIFDRVTDIWMGNEEEGYLRLDYSESNKSL
                     YRITTNIYNAAFLKIIGKYTYQVLNVIPKDLNGNPIDDLTAARVDADKNQSGVQELKE
                     WIGVMEYIKSFPDTSGDGIPDVPEKYRGKLGRIVVQASWNPVSLLSKGTMVTWGAFSA
                     IVICLLLLALIAGYGVRVARRRRGSQGLT"
     gene            complement(5276..5932)
                     /gene="nth"
                     /locus_tag="VEL68_04870"
     CDS             complement(5276..5932)
                     /gene="nth"
                     /locus_tag="VEL68_04870"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXZ35331.1"
                     /translation="MTLEEFIQIWPRLTKQVRSLRIPWVDQMSSEDRDPFKILISCIL
                     SLRTQDKTTGEASQRLFRMAQTPEALSKLAVEKIQKAIYPVGFYRVKARRIKDLGEKI
                     VEKYQGRVPDTIEELLTLNGVGRKTANLVVTLGYDKPGICVDTHVHRITNRWGLIKTK
                     NPAQTEFALREILPVRYWKKLNGYLVAFGQGVCKPISPLCSKCKISASCARVGVGRSR
                     "
     gene            complement(6081..6953)
                     /gene="sucD"
                     /locus_tag="VEL68_04875"
     CDS             complement(6081..6953)
                     /gene="sucD"
                     /locus_tag="VEL68_04875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011821391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXZ35332.1"
                     /translation="MSILIDEKTRVVVQGITGRIGAIQTMHMLKAGTKITAGVTPGRG
                     GSRVEGVPVYDSVEEAMAEQEINTSVLFVPAPVAEESCMEAVDAGVKLLILITEHIPT
                     HDTMRIRAYARSHGARLIGPTTPGVVTPGKCKIGIMPTHLFTPGPVGIISRSGTMSYE
                     MAGLLSTEGIGQTTMVGMGGDLVVGTNLVELLELFQKDPETRVVVVHGEIGGTQEEKA
                     ATFIRQKMTKPVIAYMTGKVALEGSRMGHAGAVFVGKRGAMENKIKAFREAGVPIADS
                     PGEIARLVRQALKA"
     gene            complement(6950..8083)
                     /locus_tag="VEL68_04880"
     CDS             complement(6950..8083)
                     /locus_tag="VEL68_04880"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXZ35333.1"
                     /translation="MLLYEHEAKRLFKDQGVSVPPGEVAKTSLEAAEIARKMGKRVII
                     KAQVLALPGIGAGDAQIAGSPEEAGRVAEGLLGKSYNGQRVSELLVEEALPSQKEIHV
                     AIRVDGAAGAPLVMASLERLSDLDEEAKSSAKVASSHADPFLGLKSHLARKLAKALGF
                     TGDAAVKLADQVQRIYRVYAKWNALTAGFNPLVITPEENFFASGAFLEIDDSALFRFP
                     QFRELSLSRILDPLAREGKTIGVTYVGLEGDIGVICTGAGIGMATVDLISKGSCPANF
                     LETGGGITEKQMADVMRLVLKKPGLKGVFLNMYGGINPIHEGAKGVVKVIREDKVNIP
                     VVAKAIGNFQEETWKVLEEGGVTVIKTIPTDQAVEELFKRLGK"
     gene            complement(8484..9200)
                     /locus_tag="VEL68_04885"
     CDS             complement(8484..9200)
                     /locus_tag="VEL68_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ35334.1"
                     /translation="MPSKGAALAKELKDFSLSRGADLVGIADLVPVRNFILSQGPSMV
                     GQFPKAISMGMRLNDWIVDSHTPHEPRRHSLYWHHVYDVVTQSLDFLAYDVTRWLTSR
                     GFQAFPIPGSTPYDFDKLLGIFSHKLAAHLAGLGWIGKSCLLLTEQFGPRVRFVSVLT
                     DAPLDTGSPLDKPCGKCHVCIDACPVKAFTGVEFNPAEGRGVRFDVQKCSEYRREHPC
                     GLCVSTCPTGRKGRGKALNP"
     gene            complement(9422..>9676)
                     /locus_tag="VEL68_04890"
     CDS             complement(9422..>9676)
                     /locus_tag="VEL68_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35335.1"
                     /translation="SHSPSQRASGLEDLFASHLQLFLQSRKNGLGSAEIGHVIMEGQL
                     EDLFQKFLSVFHHFLTSMIDYQRIPFSTMINITHRIIKNG"
BASE COUNT         2143 a         2666 c         2541 g         2326 t
ORIGIN      
        1 ttgtaggtga aagaatcaac tccgacgggc aggcgtggaa gggatgacgt gataccgaga
       61 tttctgtttt caaaggagca tgaaagggtt ggagcattca acgcgcatcg gaattaatcc
      121 ccccaatccg cgtcctgagg ccacattatc atctttcttc ggcggggtct ttaagaggtc
      181 gagcaggtcc cttttggtat tgaccctaca cgtttccttc atgccagcct tgaaaatctt
      241 ctccggcaaa gaatccttag gcttcatttt ttttggcttt tgcccccgtc ttcatgattt
      301 cgataaagcc tcttaaggtg tcccgcctgc ccgaggatct ttccatttca agggcgtcga
      361 gtacgccctt ccttgcagcc ggattaaaat tcccttccct taagcatccc agctattgga
      421 taatgataac agaggccatg aatgatggaa tgggcgctcc agaaaaacaa cctcgaggga
      481 agaaatgacc ttagccatta tatataggga agagctgaag gaatacgatt tcgggcccgg
      541 ccaccctttc cggggagacc ggtacgagat ttttccgaaa ttcctccagg agaaactgcc
      601 ggccaaaaac cattatcagt tcctgaaagc cgaatgggct tcggatgagg atctgcttct
      661 catctgccgg agagaataca tcgatttttc cgcgacgtat taccggtcgg cgaatctggg
      721 acaggaatac gggagcctgt tccttcaata ccacagcggc gacaaccggc cccacggccg
      781 gccgggaaaa ttggaggaag ctgcccggtt gattgtggga caggcgaaaa aagcctgcga
      841 cctggtggaa aagggaaagt tccccaaagc ggtttgcctg ggcgggggca tgcaccacgc
      901 caaacccaac tgcggtgaag gattctgcgt ctacaatgat gttgccttcg caggaaaata
      961 tcttctaaaa aaatacggcc tccagagaat cctcattctg gataccgacg cccacaccgg
     1021 caacggaacg gcggaatatt tctacgagga cccccgagtc ctcttcatcg acatccacca
     1081 ggaccctcac accctgtatc caggcacggg gttcacccac cagatcgggg aaaaggaagg
     1141 gaaggggtac accattaacc tgcccatgcc tccctatgcg ggggatgaat gctaccggct
     1201 ggctttcgag aaagtcatct tgccggtcac ttcagaattc aaaccccaga tcattctgcg
     1261 aaacggcggt tccgatccgc attactccga caacttgacc aatttggggc tcaccatcca
     1321 agggtttcgg atgatcggcg agaaggtgcg cgaaatggcc caaatctgcg gggggaaagt
     1381 ggtggatatg atcggttcgg gatacaacca agacgtcctg ccttacgcct ggttcgctct
     1441 gatttcgggg ctggccaatt tctccgttcc cgttgaagaa cccgttcatg tccccgctcc
     1501 tcgtaaagga gacccctcat tgaatgaaac caaagagatg atccaggaaa ccaaaaaggt
     1561 gctgaaggat tattggacct gcctggccgg cctttgaaca ttgcgttcga ggaacgcatc
     1621 ccacattctt gcgtaaacga actttttcgt gcggaaagga ccttcgttgc cctataagat
     1681 ggtccaccct ccgtccacgt agatcgcttg gcccgtgatg tagtcagagt attccgaggc
     1741 cataaacacc gccaggttgg caatatcctc gggttttccc agccggttca aagggatccg
     1801 ctccagtaga aactggcgaa cgggctcctg ggagaaatag gattcggtca tttcggtaac
     1861 ggtccatccc ggcgcaaggg tgttgaccgt aattccatgc ttcgcccatt cggcggccaa
     1921 gtccttggtg agggcgatga ttcctccttt actggcgcaa taggcagctc tgggcggcag
     1981 ggctacttct cccataatgg aggctaggtt gatgatcttc cctcccttcc cctctcgaat
     2041 catggctttg gcgacctctt gcgcgcagag gaaagttcct ttgagattga tatcgatcac
     2101 tttatcccat tcttcttcgg atatctcttc ggctggtttc ctcaagatga cccctgcgtt
     2161 gttaatcaga acctggaccc gacctaattc tcggatcgta ttttgaacca tctgttcgat
     2221 ttcgcttcga atgcgaacgt cagcctttgc cgacagcgct ctccctcccg aagattctat
     2281 ctccctggac acctgcgttg cagcattcca atcaacatcg ttcaccacaa cactcgcctt
     2341 agccgccgcc aaggctttgg caatggcctt tccaatcccc ctccccgcac cggttacgat
     2401 ggcaactttt cccgtcaaat tgatttgcaa atgtctcacc ttctttcccc gaatggatgg
     2461 ccttgaccag actgcacctt ttggcggatt gaatatacga aatgtgaggg agaccgtcaa
     2521 gcccgatatt gcccgatatt gaatcttttc aagtcgatct ggagacagtg atccctgcct
     2581 tttctccaat ttctgattga gagagccaat gaccctcttc ggtaaatttc aatggatcga
     2641 ttcctcggtg gtgcggtaag attcccttaa ctgacattta tcttttattc atttaaagac
     2701 gatggatgat taaatggcaa tgtttttgct tcgatcaaaa agggatggag tctatgagat
     2761 ttgctaaatt cgtggttctc gcaatgctgc tttctttctc ctatggatgt ggaggtggtt
     2821 caggatcgga cagttcgtca tccggaggtc cgggcggtgg aggggctgga acgggcggag
     2881 gagggggcac caccgggtcg agccatcccg ccgcggccga ggattgcgtt gaaaccatca
     2941 accagtaccg ggcctccatc ggactggcac cctatgcccg tttgaccgat gccgaagcct
     3001 gcgcggacga cgaggccagg agagacgccg aggcgaatca accccatggt tcctttgggt
     3061 cctgcagcga gcgggctcag aacacctgcc ccggctggcc tggtaccccc gagaccatga
     3121 tcggttcgtg cctcgcggcc atgtgggccg aagggcccgg ttcggatttc tccacccacg
     3181 ggcactatat caacatgtcc agcacgagtt atacaaaagt agcctgtggc ttctacgtga
     3241 cttccagcgg ccgggtctgg acggtgcagg acttcaggta aggccctgcg aacccctcct
     3301 tcttctggcc accctcaccc catagccggc gatcaaggcc agaaggagca ggcagatgac
     3361 aatcgcggaa aaagcacccc aggttaccat cgtccccttc gatagcagag aaaccggatt
     3421 ccagctggcc tggacgacga tcctgcctag tttgccccgg tatttttcgg ggacatcggg
     3481 aatcccatca ccgctggtgt cggggaagct cttgatgtat tccatcaccc cgatccactc
     3541 cttgagctcc tggaccccgg attggttctt atcggcgtcc acccgggcag ccgtgaggtc
     3601 gtcaatcggg tttccgttca agtccttggg gatgacattg aggacctggt aggtgtattt
     3661 tccgatgatt ttgaggaagg ccgcgttgta aatattcgtg gtgattcggt acaggctctt
     3721 gttggattcc gaataatcca gccgaagata tccctcttct tcatttccca tccagatatc
     3781 ggtcacccgg tcgaaaatca cccgccgggg gttgtaggtg aatttcaagc ccgaaacctg
     3841 caggaaatag gagctccctt tgcggggata gacgctggtc aaaacttcca gggccttctt
     3901 gatttccgag gcgtaaaagt aacagctgat cagagggtag cccatggtgt cgtccatgcc
     3961 gatgcccagg ggaattgcgg cgaagacgtc ggttaccgcc agggcgccgg ttttaccccg
     4021 tacaagatta tcccggatga ggccgttgga ctcgatggcc accgcgacct tggtccgggg
     4081 atccccggga tcggaatcat gcttgtttac ccaccaccga atagcatcgg cgatgagatt
     4141 gccgagattc gactcctcct ctttgatcgt gaggtcgaag gcgctctgcc caatcacttt
     4201 cttggaggtc aagttcacct tctggagaac atcccggtcg atggttccca ggaaggaatt
     4261 gatctttttt tgcagagcca tgtccccggg gatggatgaa tcgatatcga tgagccggta
     4321 ggtcttctgt ctgaccttcc cgctctccca gagaaggtcc aggactccga cgcattttcc
     4381 gtaggctgag gcttgaagga tcaaagtgga attgatcaag atgggtttct ccagccgggt
     4441 gtggctatgg cctcccacga tgacatcgat acccggaacc ttttgagcca gggcctcgtc
     4501 ttccgagatc gacttcgcct ccctcacccc gctgtgggaa aggcagatga ccatgtccac
     4561 cttttctttt tcccgcaacg ccttcaccat ttcccgggaa gtttcaatgg ggtctcggaa
     4621 tgtaacgggt gaggcaaagg gagacttctc cgcggcatcc tttcccatga tcccgaaaat
     4681 cccaattttc atccctccct tttgcaagac catgtagggc tttaccaggc cttggttgaa
     4741 tgcctcttcc agagcgtcgt cccggtcgct ctccttgctg aaaacggcat tggaaaaaac
     4801 aatgggaggc attccgccct tggccgccgc cgaatggaga atccgggcta ggcctttcgg
     4861 cttgaggtcg aactcgtgat ttcccagggt catagcatca tatcccatgt ctttcatcag
     4921 ccggagttcc atggcctctt cccgggccag catatggaaa agcgaaccca tgaggaaatc
     4981 tccgccgtcg agcaccagca caggatgatc tctcttctcc ttttcccgtt ttatgcccgt
     5041 agcgaccctg gccaagccac ctttggtctc gtccccgccg gtcatatccg gccggtagtc
     5101 gaggttgggg gaaaacccga gaatatggga atgaaggtcg ttggtgtgga tgatggtgat
     5161 ttctttctgc cctgcaaaaa gatcggcgga cagaaagaga agaatcaggg aaatggaaat
     5221 tctcaataat ttcatccttt cattctccct ctggattcaa atttccgggc aggcattacc
     5281 gactgcgacc cacacccact cgggcacaag aggcgcttat tttacacttg gagcacaggg
     5341 gagagatggg cttgcagacc ccctggccga aggctacgag atagccgttt agctttttcc
     5401 agtagcgaac gggcaggatt tcccggaggg cgaattcggt ctgagcggga ttcttagtct
     5461 tgatcagccc ccatcgattg gtgatgcgat ggacatgggt gtccacgcag attccgggtt
     5521 tgtcgtaccc gagagtaacc accaggttgg cggtctttct tccgacacca ttgagggtaa
     5581 gcaattcctc aatggtgtcg ggaacccttc cttggtattt ctctacaatt ttttccccga
     5641 ggtctttgat tctccgggct ttgacccggt agaagcccac ggggtagatg gccttctgaa
     5701 tcttttctac ggcgagtttg gaaagcgctt ccggcgtttg agccatccga aacaagcgtt
     5761 gggaggcctc cccggtggtt ttgtcctggg ttctcaggct taatatacag gagatcagaa
     5821 tcttgaacgg gtcccggtct tcggaggaca tttgatccac ccaagggatc cgaagggacc
     5881 tcacctgttt cgtcaaccgg ggccagattt gaatgaattc ttcgagagtc attggttctc
     5941 ccaagttttc accccccggt ttgctgaccc ttccgcttaa aataggtcag gcatcgaaac
     6001 ggctcggatg ggcgggcttt cgaggggaaa aaatagaccc caaagaaaag tctggggcct
     6061 ttcgaagggt caacctgaaa tcaagctttc agggcttggc gcaccaacct ggcaatttca
     6121 ccgggactgt cggcaatggg aacccccgct tcccggaagg ctttgatctt attttccatg
     6181 gcccctcttt ttccgacaaa aactgccccg gcgtgcccca tgcgggatcc ttcgagggcg
     6241 accttcccgg tcatataagc gatgaccggc ttggtcattt tctggcgaat gaacgtcgct
     6301 gctttttctt cctgggtccc gccaatctca ccatggacga ccaccacccg ggtctccggg
     6361 tctttctgga atagctccag cagctcaacc aggttggtcc ccacgaccag gtctcccccc
     6421 atccccacca tggtggtctg cccaattccc tccgtggata acagtccggc catttcataa
     6481 gacatggtgc cgcttcggct gataatcccc accggaccgg gggtgaagag gtgggtgggc
     6541 atgatcccga tcttgcattt tcccggagtg actactccag gggtggtggg cccgatcaac
     6601 ctcgccccgt gactccgggc ataggcccgg atgcgcatgg tgtcgtgggt cgggatgtgt
     6661 tcggtaatga gaatcaggag cttcacccct gcgtctacgg cttccataca ggattcttcg
     6721 gccacgggcg cgggcacgaa gaggaccgag gtattgattt cctgctccgc catggcctcc
     6781 tcgacggagt catataccgg gacgccctcg actcgcgagc cgcccctgcc cggggtgacg
     6841 ccggcggtaa tttttgtccc ggccttcaac atatgcatcg tctggatcgc tccaatccgg
     6901 ccggtgattc cttgcaccac aactcgggtc ttttcatcta tcaggatgct cattttccca
     6961 acctcttgaa gagttcctcc acagcctgat cggtggggat ggtcttgatg accgttaccc
     7021 ccccttcttc taggaccttc cacgtttctt cctgaaaatt cccaatggcc ttggccacca
     7081 cggggatgtt caccttgtcc tcccggatca ctttcaccac tcccttggcc ccttcgtgga
     7141 tcggattgat tcctccatac atgttcagaa atacgccctt caagccgggc ttttttaaca
     7201 ccagccgcat cacgtcggcc atctgttttt ccgtgatccc tccgcccgtt tccagaaaat
     7261 tcgccggaca gcttcccttg ctgatcaggt cgaccgtggc cataccaatg ccggccccgg
     7321 tgcagatgac cccaatgtcc ccttccagcc cgacgtaagt gaccccgatg gttttccctt
     7381 ccctggccag ggggtcaaga atgcgggaga gggaaagttc ccggaactgg ggaaaccgaa
     7441 aaagagcgga atcgtcaatc tcaaggaatg cgccagaggc aaagaaattt tcttcggggg
     7501 ttataaccag gggattgaat ccggctgtca gcgcattcca tttggcatag accctgtaaa
     7561 ttctttggac ttgatcagcc agtttgaccg ccgcatcacc cgtgaacccg agagccttgg
     7621 ccaatttcct ggccaaatgg gatttcagcc cgaggaaggg gtcggcgtga gaactggcca
     7681 ccttggccga acttttggct tcttcatcca ggtcactcaa acgttcgagg ctggccatca
     7741 ccaggggggc acctgccgca ccatccactc ggatcgcgac atgaatctct ttttgagacg
     7801 gaagtgcttc ttcaaccagg agttctgaaa cccgctgtcc gttataggat ttccccaaga
     7861 gtccctcagc aactcggccc gcctcttctg ggcttcctgc aatttgggca tcccctgcgc
     7921 ctatcccagg gagcgccagg acctgtgctt tgatgatcac ccgcttgccc atttttcggg
     7981 caatctctgc ggcctccaga gaggtctttg ccacctctcc cggggggacg gagacaccct
     8041 ggtccttaaa aagtctcttg gcttcgtgct cgtaaagaag cattttttat ccccttgtgg
     8101 ttaaatggaa ggacggtttg atgaaaaaaa gaccccgatt tcctttcaga aatggggtca
     8161 cccctttatt cccatccggg cctcctaatt ttttcaatgg acccaagaat aaggattaga
     8221 gaatacttgg gaacgaaatt cgagcagagt atactaaaaa aggaaaaaga attcaaatgg
     8281 atttaatttc cagggccgtg ttcgatcaat aagggagcag attggcgagc gcaaagggaa
     8341 aaggaatgat ggctcatgta tgaagcaatg gcagaatgaa taataatgat tcaaaatggc
     8401 caataaataa acaatagctg taaattgatt tcgtggtctg gtctcccacg gtaaaagaat
     8461 aggtctgagt tcgaaaaatt gcgtcaggga tttaaggctt tgccgcgtcc cttcctgccg
     8521 gtcgggcagg tggaaacaca gaggccgcag gggtgttccc ggcggtactc gctgcatttt
     8581 tgcacgtcaa accggacccc ccgtccctcg gcaggattga actcaacccc ggtaaaggcc
     8641 ttcaccggac aggcatcgat gcagacatgg catttgccgc agggtttatc caggggtgag
     8701 cctgtatcga gaggagcatc agtcaggacg gaaacgaatc ggactcgagg gccaaactgt
     8761 tcggttaaaa gcaggcagct cttgccgatc caccctaaac cagccaggtg ggcggccagc
     8821 ttgtgagaaa aaatccccaa gagtttgtca aagtcgtagg gggtggaacc cgggatcggg
     8881 aatgcctgga acccacggct tgttagccaa cgggtaacgt cgtaggccaa aaaatccagg
     8941 gactgagtaa cgacgtcata gacgtggtgc cagtacaggc tatgccggcg tggttcatga
     9001 ggggtgtgag aatctacgat ccagtcgtta agccgcatgc ccatggatat ggccttggga
     9061 aactggccga ccatggacgg cccctgggag agaatgaaat ttcgtaccgg gaccaggtcg
     9121 gcgattccaa ccaggtcggc tcctcgggaa agggagaagt cctttaactc cttcgccagg
     9181 gccgctcctt tgctcggcat attacttccc tcctgcatat caccccgcag ccatggagtt
     9241 gaaataattt tccctattct ttatttccac tcctggggaa agggcttaac cgtttctgga
     9301 cctttatagt ggtaacagcc ctgcaaaaaa gtcaagcagt tttcccgccg aagcagaggg
     9361 tccgatcgca aattctccct tccaatttat cgttctttgc ggtaattttt ccttagatca
     9421 tctacccgtt ttttatgatc cgatgagtga tgtttatcat cgttgaaaaa ggaatccgtt
     9481 ggtaatctat catggaggtt aaaaaatgat gaaaaaccga gagaaacttc tggaagaggt
     9541 cttcgagttg gccctccata atgacatgac ctatttcggc tgaacccagt ccgttcttgc
     9601 ggctctgcaa gaaaagctga aggtgggaag caaagaggtc ttcaaggccg gaagcgcgct
     9661 ggctggggga gtggct
//
LOCUS       DASPRR010000133         5405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_39214, whole genome shotgun sequence.
ACCESSION   DASPRR010000133 DASPRR010000000
VERSION     DASPRR010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39214"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1430
                     /gene="yedE"
                     /locus_tag="VEL68_04895"
     CDS             342..1430
                     /gene="yedE"
                     /locus_tag="VEL68_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HXZ35336.1"
                     /translation="MSNLMSFFASRWGIISAGIFIGVLAPILQKLGNPANMGVCVACM
                     ERDIAGALGLHRADVVQYLRPEIMGFVLGSLIAAMLFREFKPRTGSAPIIRFFLGFFA
                     MVGALVFLGCPWRALLRLAGGDGNAILGLAGLASGIWIGVLFLRGGYNLGRSTPTQAA
                     AGWIMPILMIVLLLFALFEPQFAQGGPIFKSAKGPGASHAPLIISLLVGLGVGFLAQR
                     SRFCTMGAIRDVILIRDFHLFSGILALVVSAVAINLLLGQFKPGFANQPIAHDNHLWN
                     FLGMSLAGLAFALAGGCPGRQLILSGEGDGDAGVFVVGMVVGAGFAHNFALASSAKGP
                     SVFGPAAVIIGLIFCLMIGFAMRERSKS"
     gene            1427..1642
                     /locus_tag="VEL68_04900"
     CDS             1427..1642
                     /locus_tag="VEL68_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXZ35337.1"
                     /translation="MNEKIDARGLSCPQPVILAKKAIDKIGKGTIEILVDTVTSRENV
                     SRLARSAGCRVSVEEKEGEFLLKLLKA"
     gene            1664..2818
                     /locus_tag="VEL68_04905"
     CDS             1664..2818
                     /locus_tag="VEL68_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXZ35338.1"
                     /translation="MIYFDNAATSFPKPEEVARAMVHFSNEIGGSPGRSGHRLAIEAG
                     RVVFEAREGIARLFGVKDSSRAVFGLNATEGINQGLKGLLRPGDHLITSSMEHNSVMR
                     PLRAMMKEGLEVTILNCSPEGLLDPSDVKKAIRPNTRMVVLNHASNVVGTIQPLSEIG
                     EVCRRQEVLFFVDAAQSAGAVPIDMEQEKIDLLAFTGHKALFGPQGTGGLVLGERVDE
                     KELVPVKRGGTGSRSEQEEQPDFLPDLCESGTPNAVGLAGLLAGLEFVMKVGIGKIRH
                     HEKRLTEKLLQGLLKIEGVTVYGPGTAEKQLATVSFNLKGILPSEVGLRLDEDFGILC
                     RVGLHCAPAAHRTLKTFPEGTVRFGLSYLNREVEVEEALQAVARIARRGA"
     gene            2821..3048
                     /locus_tag="VEL68_04910"
     CDS             2821..3048
                     /locus_tag="VEL68_04910"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HXZ35339.1"
                     /translation="MPQGVILFPSIHFALRAEKMVKEKGISYKLIPVPRHLSSDCGVC
                     LRIPWEKKEEVLEILVQAGVKVDSAHPLSDG"
     gene            complement(3311..4945)
                     /locus_tag="VEL68_04915"
     CDS             complement(3311..4945)
                     /locus_tag="VEL68_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35340.1"
                     /translation="MAGFKEGRALLGLCRNGCLTLFFFILLFYTSAGYAAAEEKPQRG
                     GTLIFAVGGEPPTYDAHRESTFGVIHPISPHYSLLLKFDEDRYPNVVGDVAESWTVSK
                     DQKTYTFKIRQGVKFHDGSILTSKDVKATYDKIVFPPQGVISPRSAFYSCVEKVEALD
                     DSTVVFHLKWASVSFLSSLASPWNYIYKAEILAKNIRWYERNIMGTGPFKFVEHVAGS
                     HWVGKRNEEYFQKGLPYLDGYRALFIMDTSARVSAIRAGRVHVEFRGFPPTQRDDLVR
                     AMGDQVRVQENSMTTCISLIFNTEKKPFNDPRVRRALTLALDRWEGSKVLSKMSIGKW
                     VGGLLRPGSEFALTEEELVQIPGYSRDIQTSRKEARRLLREAGVPEGFSFELDNRPTA
                     MPYEIDAIWVADQWRQVGLNVKQKVQDYGAMLNIYRAGNFGAGVYNISDYMDEPDLQF
                     TWSLSSDKSPTNYGRYKDPILDDLYQKQNRTTNPVERRNLCKQYQQRVLGEMAYCFPT
                     FWWHRIVPHSAKLKGVKLLPSHFLNQDLSHLWLSKE"
     gene            complement(4963..5202)
                     /locus_tag="VEL68_04920"
     CDS             complement(4963..5202)
                     /locus_tag="VEL68_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35341.1"
                     /translation="MVVELDSLELLFNSEQGYTTLENVLIGIAFSGRKGDLKEAKRLL
                     AGVGLPHRLKYYPSQISFEEQQRVTVGKVGRRSSF"
BASE COUNT         1126 a         1467 c         1600 g         1212 t
ORIGIN      
        1 agctgggccg ccccgcccct cctagtctgg gaaaaatggg taaaaatacc catttacaaa
       61 aaggagagca gggcattaga ataaccacaa taaaacccgg aaggtggtga gaccatgggg
      121 aagctccaga cccccctttc aattcgatga gggcttcggg gaagccctca gaggcctagg
      181 ttaggtctca ggccaaatcg aaggggagag ggaatggaga ctgcccccat aatacttgaa
      241 agacccttcc tttaaaaacc tcccttccct cctttttcct tcgaatggtc attgccaatc
      301 agtcaactca gaaaagcctt tttccttgag gagggaaatt catgtctaac ctgatgtctt
      361 tcttcgcctc ccgctgggga atcatttcgg cgggaatctt catcggtgtc ttggccccga
      421 tcctgcagaa gctgggaaac ccggccaaca tgggagtctg cgtggcctgc atggagcgcg
      481 acattgccgg ggccctcggt cttcaccggg ctgatgtggt gcagtacctc aggccggaaa
      541 tcatgggatt cgttcttggg tcgctgatcg ccgccatgtt gtttcgggaa ttcaaacccc
      601 gcaccggctc ggctcccatc atccgtttct tcctgggatt cttcgccatg gtgggggcac
      661 tcgtattctt gggctgtccc tggagagccc ttcttcggct ggctggcggg gatgggaatg
      721 caatcctggg gctggccggc cttgcctcgg ggatctggat cggggttctt ttcctgcgag
      781 ggggttataa cctgggaaga tcgaccccca cccaggcggc tgcgggatgg atcatgccga
      841 tcctcatgat cgtccttctt ttattcgccc tttttgaacc ccagttcgcc caggggggcc
      901 ccatctttaa aagcgcgaaa gggccgggcg cctcccatgc tcctctgatc atctctctcc
      961 tggtgggatt gggggttgga ttcctggccc agcgcagccg gttctgcacc atgggagcca
     1021 tccgggacgt gattctcatc cgggatttcc acctgttcag cggaatcttg gctctggtcg
     1081 tatccgcggt ggccatcaat ctgcttctgg gacaatttaa gcctggcttt gccaaccagc
     1141 ccattgccca tgacaaccat ttatggaatt ttctgggcat gtcccttgcc gggttggcct
     1201 tcgccctggc cggaggctgt ccgggccggc aactcattct ttccggcgag ggagatgggg
     1261 atgccggggt ttttgttgtg ggcatggtcg tcggggcggg attcgcccat aactttgccc
     1321 tggccagctc ggccaaaggg cccagcgttt tcgggccggc ggcagtgatc atcggtctga
     1381 ttttctgcct tatgattggc tttgcgatgc gggaaaggag taaatcatga acgagaaaat
     1441 agatgcccgt ggactgtctt gtccgcagcc ggtgatcttg gccaagaagg ccatagataa
     1501 aatagggaag ggaacgattg aaattctggt ggacacggtg acctcaagag agaatgtttc
     1561 ccgcttggcc cgcagtgccg gttgccgagt aagcgtggaa gaaaaagaag gggagtttct
     1621 attgaagcta ctgaaagcgt aggtctcttc catgggagaa aagatcatct attttgacaa
     1681 tgccgcaact tcttttccga aaccggaaga agtagcgcgg gccatggtcc atttttcaaa
     1741 tgagatcggg ggcagcccgg ggcgctccgg acaccggctg gctatcgagg ccggccgggt
     1801 cgtctttgaa gcccgagagg gaattgcccg gctcttcgga gtaaaagact cctcccgggc
     1861 ggtctttgga ttgaacgcca ctgaagggat caaccagggg ttaaaggggc tgctccggcc
     1921 cggggaccac ttgatcactt cgtccatgga acacaattcc gtcatgcgcc ccctaagggc
     1981 tatgatgaag gaggggctgg aggtcaccat tctcaactgc tccccggaag ggctcctgga
     2041 cccttcggac gtgaaaaagg caattcggcc gaatacccgg atggtcgtcc tgaatcacgc
     2101 gtcgaacgtt gtcggaacca tccagcccct gtctgagatc ggagaggtct gccgccgaca
     2161 agaggtgcta ttttttgtgg atgccgccca gtccgccggg gctgttccca tcgacatgga
     2221 gcaggagaag atcgaccttc tggcctttac cggccacaag gccctcttcg gcccccaggg
     2281 gaccgggggc ctggtcctcg gggaaagggt ggatgagaag gagctggttc cggtgaaaag
     2341 gggtgggacc gggagccgtt ccgagcagga agaacaaccc gatttcctgc ccgacctgtg
     2401 cgagagcgga actccaaatg ccgtaggact ggccggcctc ctggctggat tggaattcgt
     2461 tatgaaggtg gggatcggga agatacgcca ccatgaaaaa cgactcaccg aaaagctcct
     2521 tcaggggctg ctgaagattg aaggagtaac ggtgtacggg cccggaacag cggagaaaca
     2581 gctggccacc gtttcattca accttaaggg gattttgcct tccgaggtgg gcctgaggct
     2641 ggatgaagac ttcgggattc tttgccgggt ggggctccat tgcgcccccg ctgctcaccg
     2701 caccctgaag acctttcctg aagggaccgt gcgcttcggc ttgagttact tgaaccggga
     2761 agtggaagtc gaagaggctc ttcaggcggt ggcccgcatt gcccggaggg gggcttgaag
     2821 atgccccagg gagtcattct cttcccctcg attcattttg ccctgcgggc ggaaaagatg
     2881 gtgaaggaga agggaatttc atacaagctg attcccgtcc ctcgccatct cagctccgac
     2941 tgcggagtct gcctgcgcat tccctgggag aaaaaagaag aggtgttgga aattctggtt
     3001 caagccggcg tgaaagtaga cagcgcccat cccttatcgg atggatgaaa ggtttttggg
     3061 tgtttaaggc gcctgatgta cggcgacgca tattcgaaat gccttgccgg gaatgaaagg
     3121 ggatgggtgg ttgatgcagt gaaaccgaat atttattgga attaacggag ttgcgtcctt
     3181 ctcattccca aacggatcgt tgcgctgtat gcgggtggat taatagtctt cgagtttcag
     3241 gtttccccct ctgttccccg accgggagta gagggggttt tttattcctg attccttgtc
     3301 tgttctcctt ctactcctta ctcagccaca agtgggaaag gtcctggttg aggaagtgac
     3361 tggggagaag cttcactcct ttcagtttgg ccgagtgggg aacaatccgg tgccaccaaa
     3421 aagtcgggaa acagtaggcc atctcaccca ggacccgctg ctggtattgc ttgcagaggt
     3481 tccgccgctc taccgggttc gtggtccggt tctgcttctg gtagagatca tccagaatcg
     3541 ggtctttgta acgcccgtag ttagtcggcg atttatccga ggacagggac caggtgaatt
     3601 gcaggtcggg ttcgtccata tagtcggaga tgttataaac cccggcgccg aaattgcccg
     3661 cccggtagat gttcaacatg gcgccgtagt cctggacctt ctgcttgacg ttcaggccga
     3721 cctggcgcca ctggtcggcc acccagatgg cgtcgatctc gtaaggcatg gccgtgggac
     3781 ggttatccag ctcgaagctg aagccctcgg gcactcccgc ctcccggagc agccggcggg
     3841 cttccttccg ggaagtttgg atatcccgcg aataaccggg aatctggacg agttcttctt
     3901 cggtgagggc gaattccgac cccggccgca gcagcccccc cacccacttg ccgatggaca
     3961 ttttggagag gactttggag ccttcccacc ggtctagggc aagggtgagg gcccggcgaa
     4021 cccgggggtc attgaagggc ttcttttcgg tgttgaagat gagcgagatg caggtggtca
     4081 tcgagttctc ctgcacccgg acctggtcgc ccatggcccg caccaggtcg tcgcgctggg
     4141 tcgggggaaa accccggaac tccacgtgca cccggcctgc ccggatcgcc gatacccgtg
     4201 ccgaggtgtc catgatgaaa agggcccgat acccatccag gtagggcagg cctttctgga
     4261 agtattcctc gttgcgtttg cccacccagt gtgacccggc gacgtgctcc acgaatttaa
     4321 aggggcccgt tcccatgatg ttcctctcat accacctgat gttcttggcc agaatctccg
     4381 ccttgtagat atagttccag ggcgaggcca gactggacaa gaaggagacc gaggcccatt
     4441 tcagatggaa gaccaccgtc gaatcgtcca gggcctccac cttttcaacg cacgaataga
     4501 aagcggagcg ggggctgata accccctggg gagggaagac gattttgtca taggtggcct
     4561 tgacatcctt ggaagtcaaa atgctcccgt cgtggaactt caccccctgg cggattttga
     4621 aggtataggt cttctggtcc ttggagacgg tccagctttc ggcgacatcc ccgaccacgt
     4681 tgggataccg gtcttcgtca aatttgagga gcaggctata gtggggggaa atgggatgga
     4741 tcacgccaaa ggtgctctcc cgatgggcgt cataagtcgg gggttcaccc ccgacggcaa
     4801 agatcagggt cccgccccgc tggggctttt cctccgcggc cgcatagccg gccgaggtat
     4861 aaaacaggag gataaaaaag aagagggtca agcatccatt ccggcaaagc ccaagaagag
     4921 ctctgccctc tttgaatccg gccatttcca tcctcctttc gtttagaaag agcttctcct
     4981 acccaccttt cccacagtaa ctctttgctg ctcctcaaag gatatctggg aagggtagta
     5041 tttcaatcga tggggaagcc caaccccggc cagcaagcgc ttggcttctt tcagatcgcc
     5101 cttacgcccg gagaaggcta taccgatcaa gacattctcc agggtggtat acccctgttc
     5161 actattgaat agcagctcaa gggaatctaa ctcgactacc ataccctgcc ttcataaata
     5221 gggggctggc tgacggtccg aaggagttga tctaaggagg cggcgccggc aaggctgacg
     5281 gctgcaaatt tcaccaaaag cagcatggaa aaaccactcc cctttgagtc gatagaggac
     5341 aatcctgcca gcgccacagg gcttcttaga atatcttgcc tgagttaatt ccggtacaac
     5401 caaat
//
LOCUS       DASPRR010000134         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1970465, whole genome shotgun sequence.
ACCESSION   DASPRR010000134 DASPRR010000000
VERSION     DASPRR010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970465"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VEL68_04925"
     CDS             <1..605
                     /locus_tag="VEL68_04925"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HXZ35342.1"
                     /translation="LLAGGTRNIDLVDSSNPNTRWMLPASASGAALLLRRRHPRNRLL
                     GTAFCADPEMADEVYVPGGGTVRPFSPEILNTEAMFFQVARPQEVETYLAGRLAPQLV
                     SLIKKTMAQAGFAGQNPEYLALRHLRPQERARVLKGLRLRKEATDALADVGHHGPNDV
                     IISLDRGLKREVVKDGSRVVMAAAGIGFTYAAALIRWGAV"
     gene            724..1530
                     /locus_tag="VEL68_04930"
     CDS             724..1530
                     /locus_tag="VEL68_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ35343.1"
                     /translation="MPFLNLKDGAKIYYEVHGEGEPVIFLHGIMMSTLSWLAFIPELS
                     RRFKLILVDFRDQGQSSRMKEEYGQDIHVGDIVSLLDELTLPKVHMMGLSYGGQVALR
                     FALQHQGRIKSFCLFNTPNLVSNHLREIGRAWETAAALNDGERFFQLAIPFIYSEPFY
                     EKNLAFLMERQKMFKSLLTQEWFEGFIRLSRSVRGYSISREELKTIQVPTLLVGADLD
                     IIISPRSMKDIHESIPNCEFIMIPNAGHGAFLERMNEFLTILIGFVTKNS"
     gene            1579..2385
                     /locus_tag="VEL68_04935"
     CDS             1579..2385
                     /locus_tag="VEL68_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ35344.1"
                     /translation="MPFHRMNGIQVYYEEQGSGYPVLLIQGLGYPSGMWFMQVPALSR
                     RFRTIVMDNRGVGKSDKPDEEYTVSRMASDAGELLHALGIPKAHVVGVSLGGYIAQEL
                     ALNHPDLVDRLVLMATSCGSPRYWELTKALWDEIAKLAGLPPEQIIRQGMFFAATERF
                     LRDFPELIERSVGIRMENLQPLFAFTRQSNAAMSFDSRDRAHLIRQPTLILAGAQDRV
                     MPLVLTEELAKKVPQAQFKVFPHAAHLLFLEEADAVNRVLLDFLSGRKVE"
     gene            complement(2840..3505)
                     /locus_tag="VEL68_04940"
     CDS             complement(2840..3505)
                     /locus_tag="VEL68_04940"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35345.1"
                     /translation="MKGKLIPIVEKMLGQILVERHIISPLRLQAALDRQKKQNGKAKY
                     LGEILLEMGIPQERINDALDAYGKRKPLGEILVELGTLTPDQLQKVLEKQAQLAKIGI
                     RRPLGRLLVEMRYSNCETLMEALSKHFNMPVVSLMGFFPSSTLQKAVGEAFAQKHKIL
                     VLENDFRKIRIVLAEPLPFLMDEIRRAFPAGKLVEFCLANPLEVNYCLQKKFDPFVLF
                     HYR"
BASE COUNT          862 a         1019 c          993 g          891 t
ORIGIN      
        1 tccttctcgc tggggggact cggaacatcg acctggtcga ttcctccaac cccaacaccc
       61 ggtggatgct tcctgcttcc gccagcggcg ctgcccttct cctccggcgc cggcatcccc
      121 ggaaccgact tttgggaacg gccttctgcg cggacccgga gatggccgac gaggtgtatg
      181 tgcccggagg cggaaccgtc cggccattca gcccggaaat cttaaacacc gaggcgatgt
      241 tcttccaggt tgcccgcccg caagaggtgg aaacatacct ggctggccga ttggccccgc
      301 aactggtgag tttgatcaaa aagaccatgg ctcaagcggg attcgccggg caaaacccgg
      361 aatatctagc gcttcgacat ctgcgcccgc aggagagggc gagggttctc aagggattga
      421 gactccggaa agaagcaacc gatgctctgg cggacgtggg tcaccacggt cccaatgacg
      481 taatcatctc cctggatcga gggttgaaga gagaagtcgt aaaggacggg tctcgagtgg
      541 ttatggcggc agccggcatc ggcttcacct atgccgcagc cctcatccgg tggggcgcgg
      601 tctgaaagcg gatttcggat agtggatttc tccccggcct tccatttctt catttgaaac
      661 ccacaaatca atattggtaa ccgagggtcc tttcggcggg agaggagaaa tagaggagga
      721 gcgatgcctt ttctaaatct gaaagacggc gccaagattt actacgaagt ccatggagaa
      781 ggtgagccgg tcatctttct gcacgggatc atgatgagta ccctgagctg gctggctttt
      841 attcccgagc tttccaggag atttaagctg atcctggtcg atttcaggga tcagggccag
      901 tcctccagga tgaaggaaga gtacggtcaa gacattcacg taggagacat cgtcagcctg
      961 ctcgacgaat taaccctccc caaggttcac atgatgggac tttcctatgg gggacaggtg
     1021 gccttacggt ttgccctcca acatcagggg agaataaaat ctttctgtct tttcaatacc
     1081 cccaacctgg tcagcaacca tttgcgggaa atcggcagag catgggagac cgctgcagcg
     1141 ctcaacgacg gagagaggtt tttccagctc gccattcctt ttatctattc ggagcctttt
     1201 tatgaaaaaa acctggcttt cctgatggaa cggcagaaga tgtttaaatc cttattgacc
     1261 caagagtggt tcgagggatt tatccgcctt tcccgaagtg tcagaggcta ctccatatcc
     1321 cgggaagaac tgaagaccat tcaagtcccc acgcttcttg tcggggcgga cctggatatc
     1381 atcatatcgc cccgttccat gaaggacatc cacgaaagca tcccgaattg cgaattcatc
     1441 atgatcccca atgctggcca tggagctttt ttagagagga tgaacgaatt ccttacgatc
     1501 ctgattgggt ttgtgaccaa aaattcctga ttcttctgcg gccttccctc ttgggggcag
     1561 aacccgaaag gacccaccat gccgtttcac cgaatgaatg gaattcaggt ctattacgaa
     1621 gagcagggaa gcgggtaccc ggttctcctg atccagggtc tcggataccc ttcgggcatg
     1681 tggttcatgc aggtccccgc cttatctcgg cgcttccgga ccattgtcat ggacaaccgg
     1741 ggagtgggga aatcggacaa gccggatgaa gaatacaccg tcagccggat ggcctccgac
     1801 gccggcgaac tgctgcacgc cttgggaatt cccaaagctc atgtggtcgg tgtgtctctg
     1861 ggagggtaca tcgcccagga attggccctg aatcatcccg atctggtgga ccggctggtg
     1921 ctcatggcaa cttcctgcgg gtcgccgcgc tattgggaat tgaccaaagc cctgtgggac
     1981 gaaatcgcca aactggccgg actgccgccg gaacagatca ttcgccaggg tatgttcttt
     2041 gccgccacgg aaagattttt gcgggatttc cccgagttga tcgagcggag cgtaggcatc
     2101 cgcatggaaa acctccagcc gctctttgct ttcacccgcc aatccaatgc ggccatgagt
     2161 ttcgacagcc gcgaccgggc ccatctcatt cgtcaaccta ccctgattct cgccggcgcc
     2221 caggaccggg tcatgccttt ggttttgacc gaagaactgg ccaagaaggt ccctcaggcc
     2281 cagttcaagg tcttccccca cgccgcccac cttctcttcc tggaggaggc cgacgcggta
     2341 aatcgggtgt tactggattt tctttcagga aggaaagtgg agtaatccct tccagtaccc
     2401 ccgaatctgc cggaggtcct atcgggcggg gaatttcccc aaagctgtga aaaatctcgg
     2461 ccgcaaaatc tctgttggcc aatcttcgaa agataaaaac gagggccgag aggaaggacg
     2521 gggtgcgcaa aatggagcga cggaaaaaat cgttggggga atcgtcccct tttccgccga
     2581 aggaggcggt cccggcagag gatcctggct tgttttgcag gaccactccg gcaagcgggc
     2641 agagttcggg tgatcccggc gcccttgacc gccccatcca agtagtggca agaatctcat
     2701 ggttttgagc accccgtcct tgatcccggc cctacaagga gggccaacgc cgcctttcgg
     2761 cgatggagaa tgaaaaattc tcttggaacg ggtcttctct tttcaagccc gggcaggccg
     2821 gggatttaca atcctccttt catcgataat ggaaaaggac aaaaggatcg aatttctttt
     2881 gcaagcaata atttacttcg agagggttgg ccaaacaaaa ctcaaccagc ttcccagccg
     2941 ggaaagctct cctgatttca tccatgagga aaggaagggg ttcggcgaga acgatcctga
     3001 ttttgcggaa atcattttcc aagactaaga ttttatgctt ctgggcaaaa gcctccccga
     3061 cggctttctg caaggtggag gacgggaaaa agcccatcaa agagaccacg ggcatattga
     3121 aatgtttgga gagagcctcc atcagcgttt cacaattgga atacctcatt tcgacaagca
     3181 atcttcccaa gggtctgcgg atgcctattt tagccagctg agcttgtttc tccaagacct
     3241 tttgcaattg atcgggggtc agggttccta gttctaccag gatttcgccg aggggctttc
     3301 ttttcccgta ggcatccagg gcatcattaa tcctttcttg ggggatcccc atttccagca
     3361 ggatttcccc gagatacttg gctttgccgt tctgtttttt ttgccggtcc agagcggctt
     3421 gaagacgaag gggtgagata atatgccttt ccaccaggat ctgccccaac attttctcaa
     3481 cgatgggaat caattttcct ttcatcggtc cccctgaagc tttcctttta tccaacaacc
     3541 gaacagttca ctctgacctt gagattcgct ggacttttat ctcgagattc ttcccatttt
     3601 tcccggcctt taacgacttg caaaaaggcc ctgcgcctag tgtactatgg cttctgatcc
     3661 agtttttccg acccatttcg ctttaataat tcctggcaat agccaaatcc atgccgtttt
     3721 aaggaaaaaa cagaatttta aaatcccctt gatattatta ataaa
//
LOCUS       DASPRR010000135        28336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1322835, whole genome shotgun sequence.
ACCESSION   DASPRR010000135 DASPRR010000000
VERSION     DASPRR010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 28336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28336)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28336
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322835"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VEL68_04945"
     CDS             complement(<1..1463)
                     /locus_tag="VEL68_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ35346.1"
                     /translation="MDLFHNLIYGFSIALSFQNTLYCFIGCLLGTLIGVLPGIGPLAT
                     IAMLLPVTFTLPPVAALIMLAGIYYGAQYGGSTTAILVNLPGETSSVITCVDGYQMAR
                     QGRAGAALAIAAIGSFFAGTVCTMIIAMFGPVIAEIAFEFRAPEYFSLMLMGLVAAAV
                     LAQGDMSKSLAMVALGLLFGVVGTDVSTGVERFSFGITELSDGIGFVVIGVGVFAVGE
                     IISNLGEIEQRVVHTSRVSRLFPTKEDWKRSIGPIVRGTLLGAFFGVLPGTGPAIASF
                     ASYMVEKKVAKDPSQFGHGAIQGVAGPESANNADAQCKFIPMLTLGIPASGTMAMMLG
                     ALIIQGITPGPQVMVNNRDLFWGLIASMWVGNLMLLVLNLPLVGLWVSLLKVPYRLLF
                     PAIMVFSSIGIYSVNTSSIEMNLAALFGALGFIWMKLGCPAAPMLLGFVLGPMLEENL
                     RRALLISRGDPSVFFTRPISLAFIIATILILLTMAVPA"
     gene            complement(1477..1929)
                     /locus_tag="VEL68_04950"
     CDS             complement(1477..1929)
                     /locus_tag="VEL68_04950"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ35347.1"
                     /translation="MKVEFRRNQDFWAGLMFFVIGAAAVFIARDYPFGRLLRMGSGFF
                     PTLLGGILILFGLYIMVKGLRRNEKIQGNWSIRALILLPLVTVLFGVLMDRAGFIPAL
                     AVLAFGSAAASHEFKWGEVLLLTAFMTLLSVGVFILGLGLPYPLIKGF"
     gene            complement(2069..3040)
                     /locus_tag="VEL68_04955"
     CDS             complement(2069..3040)
                     /locus_tag="VEL68_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ35348.1"
                     /translation="MTRKLLVIVLGVLFCFSELAFAQAPKYPTKPIRMVVGYAPGGGS
                     DIMGRLIAPKITEALGQQVIVENRAGAAQNIAAEYVAKSPNDGYTLFMSSAALGVNVS
                     LYPKINYDPVKDFTPVAVFATSPNLLLVNPSFPAKNVTEFLAVVKKNPGKFNFSSSGS
                     GSTQHLSGELLKLMTHTDMTHVPYKGTSPSLTALASGEVDFSFSNIPGAQPLMGPGRI
                     RALAITSAKRSPLLPDLPTMIEGGLPGFVTQTWYGILAPAGTPASVVSALNAAIVRAV
                     QKEDFRARLAQLGADPIAETPEYFRKMLIEEIERWSKVVKASKAKPE"
     gene            complement(3051..3218)
                     /locus_tag="VEL68_04960"
     CDS             complement(3051..3218)
                     /locus_tag="VEL68_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35349.1"
                     /translation="MRTKLTARLGIQHPLIQAGMRPFSNNNLCVAGCRRYRRTWAVPR
                     EWRVYAAGTPK"
     gene            3515..3970
                     /locus_tag="VEL68_04965"
     CDS             3515..3970
                     /locus_tag="VEL68_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ35350.1"
                     /translation="MVLLEINGKKYEVEVSPDVPLLWVIREHLKLTGTKFGCGIGMCG
                     ACTVQVDGEAQRSCLIPAGDAQGRKITTIEGIPPDHPVKRAWVEEQVPQCGYCQPGQI
                     MQAVALLQADPHPSDGKILETMKGNLCRCGTYPYIQQAIKRAAQEVKKP"
     gene            3967..6138
                     /locus_tag="VEL68_04970"
     CDS             3967..6138
                     /locus_tag="VEL68_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXZ35351.1"
                     /translation="MTSFLTRRDLLKKSLAGAGLTVFVALTPTGYRILKAEDRAKEPA
                     QSCSLNLWLRITPDNLITVLVNKSEMGQGVYTSLPMIVADELDADWKQIRFESAPAGD
                     PFKDPVWNTQSTGGSTSVRHMFLPLRKAGAAAREMLRSAAAQTWGVPPGECATREGGV
                     RHGKSGRSLSYGELCERASKLPVPQAPPLKKEGEFKFVGKSIPRLDVAEKVNGAGAFG
                     IDTFVPGMLYAAIARPLAFGAKVKAYGRDAAERLPGVFKVVPIDRGIAVCASTLEGAR
                     KGRDALRVDWEPGAAPELGNEALEKNFTQNLARTGVTAKSQGNAAKALSEAAKKIEAT
                     YFLPYLAHATMEPMNCTAHVQKDRCDVWVPTQSQTGVLMTAQRVTGLKPEQIHVHTTY
                     LGGGFGRRGETDVVEEALQASKGAGKPVKVIWTREEDIQNDFYRPGNCCRIEGSIGAQ
                     GELTAWSHKVVVPSIFARVFPDRVQSGVDPAAVEGVANMEYEVPNLYVEYVRMDTPIP
                     VGFWRSVGSSHNAFTVESFMDELAHAAGKDPLLFRLDLLKNRPRVRKVIEVAAERGGW
                     GKPLKKGEGRGLAYHFAFGSHVAQVAEVSVQDQEPNLKVHRVTCAVDCGPFVNPAIIT
                     AQMKSGILMGLSAALRERVDFAQGGVASSNFDNYELLRINEIPEIDVHIVPSREEIGG
                     IGEPGLPPIAPAVANAVFQAAGIRVRRLPMLPMLLEGLKKK"
     gene            6234..7091
                     /locus_tag="VEL68_04975"
     CDS             6234..7091
                     /locus_tag="VEL68_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXZ35352.1"
                     /translation="MKRIFFLFLLVAALGVPVGKGIAQPAKAATQKGKDIPTLQAVKA
                     RGILVAGVTMNEPPLGFADKNGRYQGFYIDVAEALAKRIFDGKAKVEFIGIPVEKWVE
                     SLKQRRIDVLLAPLFISEEQKKEIDYSIPCFVSGGLILVKKDSKIRSYQELAGKSVAT
                     IRGTRAERMVGELLPKSRVVEFRNNADALQALNGRKVDAFAQLDIFVFYMEEKDKSLT
                     VVDLKPVHVSPVQLGVRKGDPEWRDAVDISLLEMMTTGEYRKLLDKWFGRVRGEFLDL
                     ALRTEIRTK"
     gene            7144..9552
                     /locus_tag="VEL68_04980"
     CDS             7144..9552
                     /locus_tag="VEL68_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35353.1"
                     /translation="MKKFAYPFLLCAGILFVASLAAKGSPDRETYDGSPPFSLDLLQP
                     ERAKDELPGKAPNAYTPKNPYNIFINYELGMHCVGFDISYCCIIPPYNSIQAQSVRSG
                     LHGERPRLLTPNDRVTLSYQVRDNSYSEGNKMKYWQVLKDVRGQGAMNDPGDNMANYV
                     WNHLYIYKDLQGTIPANPEKARRLYVGKEIPVNIDSGPSGKSVSGGYLDYAGENGGNV
                     VFTDSLMPEIKDVPLRLTSSHLWDALGLPLTAFNDRARKGSIRTITDRDYQPYQVAVV
                     RLGDEKGISLMAGGKLIEFFGTEPVDISNCSLCHSGKGMAAEMSRGAGLFLFDKEYEY
                     WKTHYPDVTEYIARLSATSIHILELHDQRHRTRFLADYNPAAASNRMGRVGSVNCVDC
                     HGDNMSGNLQSPRPGTTGYPAVRAKPLTEAVHSVHMRYIPMPDKAGRTQNCQACHPTH
                     WQSEKMNDFETNPYQVIDQQGNPRFSDADLRVSGGGCFLRRDAHANPEVSPPFFLNEI
                     GKWYLKEVSLKDERGKPVAKIRGLYCTNCHNHLSHELYRYDNLRDAALQDGKTLRNRE
                     IKEIIGVLAGGDKEKFKSYYADPAVETKGDPLFSYYAEHQGNILLRGVKDKEGRMAIL
                     PWNADRGKEVAYASVSAGDDWWFSPGEPHCADCHLAPFVESAGGKYFPIDQPGKYSLF
                     RYSKAHGVLACQSCHESMHGLYPVRQEGPEKTVDLTSHQQALQFSPDNQYAGPVTCAA
                     CHTVNPKGVPVQLRQTEYEADYWASVVLIHFMREDDQKMPVAELIRKYPYRKAKEIVV
                     KGWK"
     gene            complement(9675..11171)
                     /locus_tag="VEL68_04985"
     CDS             complement(9675..11171)
                     /locus_tag="VEL68_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ35354.1"
                     /translation="MDFLSSLQMGVATALQPMNLAYCFIGVLIGTLIGVLPGIGTSGA
                     VAILMPATFTMPSVSAIIMLAGIYYGAMYGGSTTSILVNIPGEAASVVTCFDGYQMAR
                     QGRAGPALGIAAFGSFIAGYFGVAMISVLAPSLARIAFDFGPPEFFGLMVLGLTLSTF
                     LGSGSMIKALLMVALGIVLGCVGTDPISGTPRLTYGAKSLLDGLDMVPVVMGLFGISE
                     VLINLEQQEQRNIYETKIRGLLPNREDWRRSIKPIARGSAIGFFVGIIPGGTAVISSF
                     VSYAIEKRISRRPEEFGRGAIEGVAGPESANNAATFGSMVPLLTLGIPSNVVMALFLA
                     ALMIHGTTPGPLLIQNHPEFFWGVMVSMVVGNLMLLVLNLPLIGIWVKILKVPYKILF
                     PLIILFCLIGAYSVNLRVIDVAVMLIFGIFGYLMKKYEYDGGPFILAFILGPLLETAL
                     RQSLIISQGSLGIFVTRPLALTSLLIALLFLLFPLLPSIRTKREKFAE"
     gene            complement(11638..12099)
                     /locus_tag="VEL68_04990"
     CDS             complement(11638..12099)
                     /locus_tag="VEL68_04990"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ35355.1"
                     /translation="MKKTDQIQSLIWGLFGLYIAFEGVQLKLGTPRAPKPGFLIFWMG
                     IIMMILSAAFFLQSFSAVQEEKKIRWQGRRWPKGIELMLALFLYVAVFHWLGFILSTF
                     FLLIFLFKGLEPQRWGMALLLSAVTIIICYLVFSVFLELQFPPGLLRPIFA"
     gene            complement(12195..13163)
                     /locus_tag="VEL68_04995"
     CDS             complement(12195..13163)
                     /locus_tag="VEL68_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ35356.1"
                     /translation="MSKRNLAMVVLLLLVVPLALGIQSAWGKYPERKIKFICAWAAGG
                     GSDVMARMLTRLVNPQLGGRVYVENITGASGLMGIREALNAAPDGYTLMLLTTTNTIA
                     PNVIKDYPSVELLEALSVAAEDPMILAVATDSPLKTAADLVAKAKANPGKVTGATAGH
                     GSPQHLANAAFAAATGTQISYVPYKGAAPALVAAAGKHVDFASAGISEAVTLKEANKI
                     RALVSLSAKRTRLYPDTPTAKELGYDIIISRWMGVGVPKGLPPEVRTLLLETFKKAME
                     TEEFKKFIAQNGLEQFHGGPEEAKAWIKNQNDYFKKVSDRAGIQPE"
     gene            complement(13458..13820)
                     /locus_tag="VEL68_05000"
     CDS             complement(13458..13820)
                     /locus_tag="VEL68_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXZ35357.1"
                     /translation="MQFRTLIPAMAFLCGWGGSVLLPSVLTLAAQDLFLGDRHKNLGI
                     ECSGCHREDPPKQKVPMEVCLGCHGDYSKVAAKTSKINPNPHDSHLGEIECEKCHHSH
                     KPSVNACSACHLMDMRVP"
     gene            complement(13953..15623)
                     /locus_tag="VEL68_05005"
     CDS             complement(13953..15623)
                     /locus_tag="VEL68_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ35358.1"
                     /translation="MKDMRGQKAITRRGFLKGATVAGGAIAGVALVSATGESQTPPRS
                     ASGKWDREADVVIVGSGASGLAAAVEAADSKAKVMVLEKLPGIGGNSKMSFGNYGTFN
                     SPEQRKGAEKDAHWYANDSADLFIKEKLLLGGYLNDAALVRVFVEQSLDGYNWLTNLG
                     FTHNGIAIYHSLGPNPMPENPLAMRMNMLWNTPYVDGAWQGPYTKGRQHTGGKYQDFL
                     AGEAGVMCLADHAKKRGAQILTQMEVLEIIRKGGLSGDVLGVRVKDIAANKVIRIRAK
                     RAVVLAAGGFTANGKMCNRYDPRLNPAVRASCQATGKPGIGCTGEVLLAAMDIGADAA
                     LLHIQQLRMSGSAVAYSGPGSALIAEKEGTFIDVDNQGNRFWMEGGDQSVYRQARLTI
                     LHEKDIYQWWGICDSAAANKVYLKGALETKSALAADTLPQLAEVIKVPAANLVATVDR
                     YNKFVEQNYDADFGQFKKFLTQKIEAGPFYAMPKTYYRHASVGGVRINVNAQVLDRQN
                     RVIPRLYSAGEMIGNLQGWERDGGCGWTTCTVFGRIAGRNASAEKPLA"
     gene            16947..17891
                     /locus_tag="VEL68_05010"
     CDS             16947..17891
                     /locus_tag="VEL68_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXZ35359.1"
                     /translation="MQSSSPAFPLQGSTNLELARLPNLITVAADVLAGYWIANGLKMD
                     SRLLWLLLATSAVYAGGCALNDYFDRDIDSPECPGQPIPSGRVEPWKALIYSLSLFTV
                     GLGASVLAGASAPHIMVLLILAAVLYNVGTKERPLLGPLNLAGYRSLNLILGMGLPFW
                     LGLSGLPSRYFPVFSFLYVFLLSWLSRFEVGGKGREITAVAFLGWANFVTILLFLSFG
                     EAMNLENLPFLALFMAFTGPALLKAGRDQTTFAAGGALKMMVLGLPLLNAVYCSGVQG
                     FAAGVPVALCTAIASLLAISKRVSCDLTQGLGWGRNSF"
     gene            18549..19853
                     /locus_tag="VEL68_05015"
     CDS             18549..19853
                     /locus_tag="VEL68_05015"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HXZ35360.1"
                     /translation="MNKSGIKDLLCLLLVCLPVILCSQAGESSPSSPYLFSDQELDNL
                     LAPIALYPDPLLAEILPASTYPEEVADAAAWLTSGGDPSGIDEKNWDEAVRAVAHYPN
                     ILFMMSGNMDWTADLGDAFLNQPEAVARSIQRLRWRAKAVGNLISNNEQTVVIEGDYI
                     QIIPAQPQYIYVPRYDPAVVYVQRPAEGISPFITFGFGLVIGGWLSMDFDWYNHHIIY
                     HGWNRHGWVNNARPYVHITNVYVNRSRPYIHQTWKHDASHGDPDRYRASRPGGPYRGR
                     YARTPDARGRATTAPRPSGGMFGYKADAPASSNRGRDSLAVIRPQMPTPGPGVGQAPP
                     RPTPGVSQKPTAPTPSTSQRQVTPAPRTSSGISRPSSGPGSAQLARTPSSAFGGYRGA
                     NEARTQSLRGQASRQSSVGAPPPPSPASRGSGPAGKATPRGK"
     gene            19935..20906
                     /locus_tag="VEL68_05020"
     CDS             19935..20906
                     /locus_tag="VEL68_05020"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HXZ35361.1"
                     /translation="METVQRKLPFFPSRSISFIVALLAAATLVFAWSAGEAGAASQRL
                     FSTPEDALKELHGAAQAKDRAAMDQIFGSSYKDLLSGDEVQDAAEFAEFVEHLAEKTN
                     LVKESDSKVTLYIGNENWPFPIPISKKDDQWFFDTEAGREEILNRRVGEDELIAILVC
                     RSYVKAQREYVLKDWEGDGVVKYAQKLRSDPGKKNGLFWRSRPGEEVSPLGELVAQAR
                     MEGYRKTKAVFREEPTPFHGYYFKVLTRQGKNAPGGGYNYVINGNMVAGFALVASPSN
                     WGKSGVMTFIVNQQGKVYQKNLGANTLKIAQAMEEFNPDKTWTLVKE"
     gene            21777..22883
                     /locus_tag="VEL68_05025"
     CDS             21777..22883
                     /locus_tag="VEL68_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ35362.1"
                     /translation="MKRSVVAILKTTPQRIFDDILRVMGMAEFKDALKGETGTILKDN
                     ISWHFPFLSANTTPWQLEGTIRALQAAGFLNLVAVHNKTVVTNAFKGLRLNKLAPIYR
                     KYRIPEKFNFLPQDMTWEVFRPKARMKVLDRIYPKGIKVPTFFAGKNILHLPTAKCHI
                     YATVTGSMKNAFGGLLTTRRHYTHSVIHETLVDLLAIQKEIHPGIFTVMDATVCGNGP
                     GPRTMIPVEKGLILASADSVAIDAVMAKIMGFDPMKIGYIRLAHEEGLGIGRVEEIEV
                     RGEDMGGMNFHFSVGNNLASRFGNLFWYGPLKRLQKVFFHTPLVNLFVMASYLYHDRF
                     WWPVKGKRIMEDVKAGTAWGKFFAGYTPMEIEKP"
     gene            22951..24381
                     /locus_tag="VEL68_05030"
     CDS             22951..24381
                     /locus_tag="VEL68_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXZ35363.1"
                     /translation="MLRKAAFFPLVVDLDGTLAKTDLLIESLFSLIKRNPFYVLVLPF
                     WLLKGRAFFKRQINQRVTLDVNSLPYDREFLDYLKGERSRGRPLFLATGNDERIARQV
                     AEHLGLFDRVLASDGVVNLSSRTKRDRLVAEFGEKGFDYAGNEKRDLEVWPSARKALI
                     VNSTKSILRRAGRVADVEKVFHRRDGALKSYLTALRLQQWPKNLLVFVPLVMAHRYNE
                     FALIGNAFLAFCAFGLCASSVYIINDLTDLSADRHHPRKRLRPFAAGELSLLWGLGSA
                     PLLLGGSMLLAWFLPLPFLGMLILYFLLNLGYSFTIKRVMLLDVITLAGLYAMRIMAG
                     SASVAIWPSAWLLAFSTFLFLSLALLKRYAELVMTHRMSGRNVQVRGYQVIDKELLVS
                     LGTGSGYVAVLVLAMYISSGTAEILYARHELIWFLCPLLLYWISYVWLIAHRGDMVDD
                     PLVFVFRNQVSRIVILLGAFIVLLAR"
     gene            24592..26352
                     /locus_tag="VEL68_05035"
     CDS             24592..26352
                     /locus_tag="VEL68_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35364.1"
                     /translation="MGLVVFSLAFITMLIISALWNPEGRADDPGVSIPKTIIELQPFR
                     QTNSIQIKSAQGREGLATLININPGVNAWFLLNLDFHEGTPAEEYHLQNTNPETQKLS
                     LDADYPYGLVVEEGVNRYTCNLWGVEPRTVLKRARASGAAYAPLCEGRLYLRNPAKGH
                     RTRIEMATDFLRDEVPGGEKIVTIVRDTFFADAYREKTKTAETPKTYAKELSPGKPGT
                     VPASALLDQKEAGRIVVSTHLGIEVLESPPHEIVLGNWYEAKDNPGIFISLIAANRIA
                     PEILRSYPKRVANLDRLEAEALVYMIAFDLDRFNLGFALGTEHPRVDWSDHAREQMKD
                     PSLPGPDGIGNIVPLVSTGLINPKDAGRTVAAFTGGFKRAHGAFRSGDLALKNHGSHY
                     GFMENGVIFSKLQPGLATIYVLNDRRVDMKTWEDEDNRLLPSIRYARQNGVPIITDFD
                     PASQMSVPGPLVSRWGEGNWSGSEDNKLRTMRAGAALQERGEKRFFLYAVFTSATPSA
                     MARVFQGYGARYAMLLDMNALEHTYLSVYRRQGSRLYVQHLIQGMSEVDKSSKGEYIP
                     RFLGFPDNRDFFYFLQKEAP"
     gene            26349..27371
                     /locus_tag="VEL68_05040"
     CDS             26349..27371
                     /locus_tag="VEL68_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35365.1"
                     /translation="MREFFVSGGILAFLWLAALLLPFPSSAQPLKEKNDALFEQLQRV
                     HGLSDDQMQSIRRIFSESGYIGQGNPAITRHPVTPQQCQEKLTLLGIRYENEEFEKFC
                     GARYMAPLYNPALENAKDASVCIDQFEFPDIPCVYPVVWVRAREAAEICWAMGKRLCD
                     AHEWEGACAGALETADYRFDLAQGLPPNTAVERMRQAHNKVYGTRKVWAYGSHYEKGI
                     CASSSQKSPSCGGGSWTQCGSNTFPAGDFPDCHSRLQVYDQHGNAAEHMNLPLNEGQM
                     SSRGSKELGYTEMKGSWFIFDTYYAHEDWCRWRAPFWHGSRVMDRHSHENYHLGFRCC
                     KTVKGN"
     gene            complement(27419..>28336)
                     /locus_tag="VEL68_05045"
     CDS             complement(27419..>28336)
                     /locus_tag="VEL68_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35366.1"
                     /translation="ISLIFAICFPSLAAFGAEWADFQDFLIRRIPFRPYWAMAGSEFA
                     KYIAKMDKTRREQAILEQLSQGNFPDFLRKLIPVPLEHILKDGKGITATIFAMPDYLG
                     VGSNRDFIRTPMNLYTAAAIADRFGFILPTTKMVDALFKQSAFHLRPEPMPPGPRMTS
                     TACYVEHNQKIQEQRLALGCPLGALISGHKKDIVITNQLAHKPERVAIYGWQHLSGTP
                     IQPLCTTHGAGYADYSHGVRLISELALLNGEPTPIFRILEDPDLAAVLSDEGAIPKVR
                     DFMARYIWQPRDPAAISSSLEPPYRSIKH"
BASE COUNT         6509 a         7684 c         7872 g         6271 t
ORIGIN      
        1 gccggcaccg ccatcgtgag cagaatcagg atcgtggcga tgatgaaagc aaggctgatg
       61 ggccgggtga agaaaacgga gggatcgccg cgcgagatca gcagcgcccg ccgtagattt
      121 tcctccagca tcggcccgag cacgaaccca aggagcatgg gggcagcggg gcacccgagc
      181 ttcatccaaa taaagccgag ggcgccgaag agcgccgcaa ggttcatctc gatcgagctg
      241 gtgtttacgc tgtagatgcc gatggaggag aaaaccatga tggcggggaa aagcaggcgg
      301 tagggtacct tcagtaagct cacccagagg ccgaccagcg gcaggttgag caccaggagc
      361 atcaaattgc cgacccacat gctggcgatc agcccccaga agagatcacg gttattgacc
      421 atcacctggg ggccgggggt gatgccctgg atgatgagcg cccccagcat catggccatc
      481 gtcccgctcg cgggaatgcc cagggtgagc atggggataa atttgcattg cgcatccgcg
      541 ttgttggcgg actcgggtcc cgccactcct tggatcgcgc cgtggccgaa ctgcgacggg
      601 tctttggcga ccttcttctc gaccatgtag gaggcgaacg aggcgatggc cggcccggtg
      661 ccgggcaata cgccaaagaa cgctccgagt agggttcctc tcacgatcgg gccgatggat
      721 cgcttccagt cctccttcgt cgggaagagg cgcgacaccc gggatgtgtg gactacccgc
      781 tgctcaatct cgccgaggtt gctgatgatc tcgcctaccg cgaatacccc cacgccaatg
      841 actacgaagc cgatcccatc cgacaactcg gtgatgccga agctgaaacg ctccacgccc
      901 gtactaacgt cggtccccac aacgccgaac agcaggccca gcgccaccat ggcgagcgac
      961 ttgctcatgt cgccttgggc gagcacggcc gcggcaacca gccccatcag catgagcgag
     1021 aagtattccg gcgcgcggaa ctcaaaggcg atctcggcga tgaccgggcc gaacatggcg
     1081 atgatcatcg tgcacacggt gccggcaaag aaagacccga tggccgcgat cgccagcgcc
     1141 gccccggcgc gcccctgacg tgccatctgg tatccgtcaa cgcaggtgat caccgacgag
     1201 gtctcgcctg gaaggttgac caggatggcc gtggtcgagc cgccgtactg ggcaccgtag
     1261 taaataccgg ccagcatgat cagcgccgcc accggcggaa gggtgaaggt aacgggcagc
     1321 agcatggcga tcgtcgccag tggcccgatg ccgggcaaaa cgccgatcag ggtacctaac
     1381 agacagccga taaagcagta gagggtattc tgaaacgata aggctatgct aaagccatat
     1441 atcaaattat gaaacaggtc catgaaaaaa atcccttcaa aatcctttga tcaggggata
     1501 aggcaggccc aggcccagga tgaacacgcc caccgagagg agggtcatga acgcggtgag
     1561 cagtaaaacc tcgccccact tgaactcgtg gctcgcggcc gcagacccaa aggccaaaac
     1621 cgccagcgcc gggataaaac ccgcacgatc catcaggacg ccaaaaagga ccgtaaccaa
     1681 gggcagcagg atcagggccc ggatcgacca gttgccctgg atcttctcgt tcctgcgtaa
     1741 ccctttgacc atgatgtaga gaccgaacag gatgaggata ccgccgagaa gagtcgggaa
     1801 gaagcccgat cccatacgca gcagcctgcc aaacgggtaa tcccgggcga tgaatactgc
     1861 cgcagcaccg atcacgaaga acatcaggcc tgcccagaaa tcctggttcc tgcgaaactc
     1921 cactttcatc gagggtcttt tctccttcct gtcagccttt tcccgcccaa gcacctatcg
     1981 acggctgcct ttttcctggg ccgagaatca aacagcaagg ggttcaacgc caaaacgttg
     2041 aacccctttt caaaggtatc ggtgaaattt attccggctt ggctttggat gccttcacca
     2101 ccttgctcca gcgctcgatt tcctcgatca gcatcttgcg gaaatactcc ggcgtttccg
     2161 cgattggatc ggcgcccagt tgcgccagtc gggcgcgaaa gtcttccttc tgcacggccc
     2221 ttacgatggc cgcattcaac gcgctgacca cggaggcggg ggttcctgcc ggcgccaata
     2281 tcccatacca ggtttgggtg acaaaacccg gcaggccgcc ctcgatcatc gtcggcaaat
     2341 caggaagcaa gggtgatctc ttggcgctgg taatggcgag cgcccggatc ctgccaggtc
     2401 ccattaacgg ctgggccccg ggaatgttgc tgaaggagaa atcgacctcc ccgctcgcca
     2461 gcgccgtcaa ggaaggcgaa gtgcctttgt aaggaacatg ggtcatgtcg gtgtgggtca
     2521 tgagcttgag caattccccg gagaggtgct gggtactgcc gctgccggag gaggaaaaat
     2581 tgaatttgcc ggggttcttt ttgaccacgg caaggaattc cgtgacgttc ttggcgggaa
     2641 atgaaggatt caccagcaac aggttcggac tggtcgcaaa aaccgccacc ggcgtgaagt
     2701 cttttaccgg atcgtaattg attttgggat aaaggctgac gttgacgccc agcgccgccg
     2761 aactcatgaa cagcgtgtaa ccgtcattgg gggatttggc gacatattct gcggcgatat
     2821 tctgggccgc ccctgcccgg ttttcgacga tgacttgctg gcccaacgct tcggtgattt
     2881 tcggggcaat cagcctgccc atgatgtcgc tgcccccgcc gggtgcgtaa cccaccacca
     2941 ttcggatggg tttggtcggg tacttcggag cctgcgcaaa ggccaattcg gaaaagcaaa
     3001 acaatactcc aagaacaatt accagcagtt ttcgagtcat gtctcctcct tcacttgggg
     3061 gtccctgctg cgtacacgcg ccattcccgc ggcacggccc aggttctccg gtatcgcctg
     3121 cagccggcaa cgcagaggtt gttattgctg aacggacgca tgccggcctg gataaggggg
     3181 tgctgaattc ccagccgtgc ggtaagtttc gttctcaaaa tattcatcct tctcagttga
     3241 ttcccttggc ttgcgtatat tttgagtgag tataaaaaaa accttcccct gtgtcaaaga
     3301 atattcacca acccttttcc accaatatca gaattcgccc cgaatcaaac cctgtatgcg
     3361 ttcggtgagc ttaagaaaga acccatccga aaaatactgt ggtatgggaa acaaaatact
     3421 gtcctccctg tattcctttt ttccttgacc ctgttaccct gaaaaaaggg agaagcaggg
     3481 acagaattgc ccaccggagg gaaggaggtc cattatggtt ttgctggaaa tcaatgggaa
     3541 gaagtatgag gttgaggtat ccccggatgt gcccctttta tgggtcattc gggaacacct
     3601 gaagcttaca ggaaccaagt tcggttgcgg catcggcatg tgcggagcct gcacggttca
     3661 ggtggatggg gaggcccaga ggtcctgcct cattcctgcc ggcgacgccc aggggagaaa
     3721 gatcactacc atcgagggaa ttcctcccga ccatccggtg aaacgggcct gggtggaaga
     3781 acaggttccg caatgcggct actgccagcc ggggcagatc atgcaggcgg tggctttgct
     3841 gcaggcagac ccgcacccct cggatgggaa gatccttgag accatgaagg gcaacctctg
     3901 ccgctgtggc acttatccgt acatccaaca ggccatcaag agagccgcgc aggaggtgaa
     3961 gaagccatga cctctttcct gacccgcaga gacctgttga agaaatccct ggctggggcc
     4021 gggctgaccg ttttcgtcgc cctgacccct accggatacc gaattctgaa ggcagaggat
     4081 cgcgcgaaag agccggccca atcgtgcagc ctgaatctgt ggctgcggat tacgcccgat
     4141 aacctgataa ccgtgttagt gaacaaatcg gagatggggc aaggagtgta cacttctctg
     4201 cccatgatcg tggccgatga actggatgcg gactggaagc aaatccgctt cgaatccgct
     4261 ccggccggcg acccgtttaa agaccctgtc tggaacacac agtcaaccgg ggggagtaca
     4321 agcgtgcggc acatgttcct ccccctccgg aaggcggggg ctgccgcccg ggagatgctg
     4381 cggagtgcgg ctgcccagac gtggggggtt cccccggggg aatgtgcgac ccgggaggga
     4441 ggggtgcgcc atggaaaaag cggacgatcc ctgagttacg gggagctttg tgagcgagcc
     4501 tcgaaacttc ccgttcccca ggcccctcct ttgaagaaag aaggcgaatt caagttcgtc
     4561 ggcaaatcca tcccccgtct ggatgtagcg gagaaggtca acggcgcagg ggctttcggc
     4621 atcgatacct ttgttccggg catgctctac gccgctatcg cccgcccgct cgctttcggg
     4681 gccaaggtga aggcctatgg acgggacgcc gcggaaaggc tccccggagt cttcaaagtc
     4741 gtacccatcg accgggggat cgccgtgtgt gccagcaccc tggagggggc gcggaaggga
     4801 agggatgctc tgcgggtaga ctgggaaccc ggcgccgcgc ctgaattggg aaatgaggcg
     4861 ctggagaaga atttcaccca aaatctggcc cggacgggcg tgaccgcaaa aagccagggg
     4921 aatgcggcca aggctctgag cgaggcggcc aagaagatcg aagcaaccta ttttcttccc
     4981 tacctggccc acgcgaccat ggagcctatg aactgcacgg cccatgtcca gaaggatcga
     5041 tgcgacgtct gggttcccac ccagagtcaa accggggttc tcatgaccgc ccagcgagtg
     5101 acgggattaa agccggaaca aatccatgtc cacaccactt atttgggcgg agggtttgga
     5161 cggcggggcg aaacggatgt ggtggaggaa gccttgcagg cctccaaggg ggcgggaaaa
     5221 cccgtcaagg tgatatggac gagggaagag gatattcaaa atgactttta ccggccgggc
     5281 aactgttgcc ggatcgaagg gtccatcggc gcccaaggcg agttgacagc atggtcccac
     5341 aaggtcgtcg ttccgtccat ctttgcccgt gtatttcctg atcgggttca atccggagtt
     5401 gaccccgcgg cggtggaggg cgttgccaac atggaatatg aggttcccaa cctctatgtg
     5461 gaatatgtcc gcatggatac tccgattccg gtcgggttct ggcgctcggt gggaagctcc
     5521 cacaacgcct tcaccgtgga aagctttatg gacgagctgg cccacgcggc gggaaaagac
     5581 ccgctcctgt tccgtctcga ccttctgaag aaccgtccgc gggtgcgtaa ggtgatcgaa
     5641 gtggcggcgg aaagaggggg atggggaaag cctctcaaga agggggaggg gcgggggttg
     5701 gcctaccatt tcgccttcgg gagccacgtg gcccaggtgg ccgaagtttc ggtccaggat
     5761 caggagccga acctgaaggt tcaccgggtg acctgcgccg tggactgcgg cccatttgtc
     5821 aacccggcca ttatcaccgc tcagatgaag agcggaatcc tcatgggtct gagtgcggcc
     5881 ctgcgggagc gggtggactt cgcccaggga ggcgtggcct cgtccaattt tgataattac
     5941 gagctcctgc ggataaacga aatcccggaa atcgatgtgc atatcgtccc gagcagggaa
     6001 gagatcgggg ggatcgggga gccgggcctc ccccccatcg ctccggcagt ggccaacgcg
     6061 gtcttccagg ccgcgggaat acgcgtgcgc cgtctaccca tgcttcccat gctcctcgaa
     6121 gggttgaaga aaaaataacc ggccgggtca agcaaggttc cgaccgaacc ttggctcgac
     6181 gggaaaagga ccaacgacca ggaaccatta tcaacaagaa caggaggggg aggatgaaaa
     6241 gaatcttttt cctttttctg ctggttgccg cgctgggggt ccctgtggga aagggaatcg
     6301 cgcagccggc caaggcggca acccagaagg ggaaagacat tccgactctg caggcggtta
     6361 aggccagggg aatcctcgtt gccggggtga cgatgaatga accgcctttg gggtttgccg
     6421 acaaaaatgg caggtatcag ggtttctata tcgacgtcgc cgaggccctg gcgaagagaa
     6481 tcttcgatgg aaaagccaaa gtggaattca tcgggatccc ggttgaaaag tgggtggagt
     6541 ccctgaaaca aagaaggatc gatgtcctct tggccccgct gttcataagc gaagaacaaa
     6601 aaaaagaaat tgattacagt attccgtgtt ttgtttccgg cggactgatc ttggtgaaaa
     6661 aggacagcaa gatcagaagc taccaggagt tagcagggaa aagtgtggct accatacggg
     6721 gcacaagggc ggaaagaatg gtcggggagt tattgcccaa atcaagggtg gtagaattcc
     6781 ggaataatgc cgatgccctt caggccttga atgggcggaa ggtggacgct tttgcgcaac
     6841 tggatatttt cgtcttttat atggaggaga aggacaagag tctgacggtc gttgacctaa
     6901 aacctgtcca tgtctctccc gttcagctcg gggtcaggaa aggcgacccg gagtggcgtg
     6961 atgccgtcga tatttcactg ctggagatga tgaccaccgg tgagtatcgt aaacttctgg
     7021 ataaatggtt cggcagggtg aggggagaat ttttggacct ggccctgagg acggaaataa
     7081 gaaccaaatg aatttttccc gggtcggaac ccgaaggaga aatgggcgga accgggggaa
     7141 gtcatgaaaa agttcgctta tccattcctt ctctgcgcgg gaatcctttt cgtggcctcc
     7201 ctggccgcca aaggatcgcc ggacagagag acctatgatg ggagtccccc attcagcctt
     7261 gaccttctcc agcccgaacg ggcgaaggat gagctcccgg gaaaagcccc aaacgcctat
     7321 acgccgaaga atccttacaa cattttcatc aattacgaac tgggaatgca ctgcgtcggc
     7381 ttcgatattt cctattgctg catcatcccc ccctacaaca gcatccaggc ccagtcggtt
     7441 cgttcgggcc tccacgggga gagaccgcgc ctgttaacgc ccaatgaccg ggtgacgctt
     7501 tcctatcagg tccgggacaa cagttacagc gaaggaaaca agatgaaata ctggcaggtc
     7561 ctgaaggatg tgcggggaca gggggcgatg aatgaccccg gcgacaacat ggccaactat
     7621 gtatggaatc acctttacat ctacaaagac cttcagggaa cgattccggc gaatccggag
     7681 aaggccaggc gattgtatgt ggggaaggag atcccggtca atatcgattc cggcccctcc
     7741 ggcaagagcg tctccggcgg ctacctggac tatgccggag aaaacggggg aaatgtagtc
     7801 ttcaccgatt ccctgatgcc ggagataaag gatgttcctc tgaggctcac ctcttcccat
     7861 ctatgggatg ccctgggcct tccgctgacc gcttttaatg accgcgcgag aaagggaagc
     7921 atccggacga tcaccgaccg tgattaccag ccctaccagg ttgccgtcgt acgactaggg
     7981 gatgaaaagg gcatttctct aatggccgga gggaaactca tcgaattctt cgggaccgaa
     8041 cccgtggaca tctccaactg ctcgctctgc cactccggaa agggaatggc cgcggaaatg
     8101 tccagggggg ccgggttgtt cctgtttgac aaggaatatg aatattggaa aacccactac
     8161 cccgatgtaa ctgaatacat cgcccgcctt tcggccacct ccatccatat ccttgaactc
     8221 catgaccaga ggcaccggac ccggttcctg gcggattaca accccgccgc ggcatccaac
     8281 cgcatgggca gggtcggatc ggtgaattgc gtggactgtc atggggacaa tatgtcggga
     8341 aacctgcaat ccccccgccc ggggaccacg gggtacccgg cggtcagggc gaaacccctg
     8401 actgaggccg ttcattccgt acacatgcga tacatcccca tgcctgataa ggccggaagg
     8461 acccagaact gccaggcctg ccatccgact cactggcaga gcgagaagat gaacgacttt
     8521 gaaaccaacc cctatcaggt gatcgaccag caagggaatc ccaggttctc ggatgcggac
     8581 ctgagggttt cagggggagg ctgcttcctg cgaagggatg cccatgccaa ccccgaggtg
     8641 tcgcccccct tcttcctgaa tgaaatcgga aagtggtacc tgaaggaggt gagcttaaag
     8701 gacgaaagag gaaagcccgt ggctaagata agagggttat actgtacgaa ctgccataac
     8761 catctgtccc acgagctcta ccgctacgac aatctccgcg atgcggcgtt acaggatggg
     8821 aagaccttga ggaatcggga gatcaaagaa atcatcgggg tcctagccgg aggagataag
     8881 gaaaaattca aaagttatta tgccgacccg gccgtggaga ccaagggaga ccccctgttt
     8941 tcatattatg ccgagcacca agggaacatc ctcttgaggg gggttaagga caaagagggg
     9001 aggatggcca tcctcccatg gaatgcggac agggggaaag aggttgccta tgcctccgtc
     9061 tccgcagggg atgactggtg gttctccccc ggagagccgc attgcgccga ctgccaccta
     9121 gctccgtttg tggagagtgc gggcggaaag tacttcccca tcgatcagcc cggtaaatat
     9181 tccctcttcc ggtattccaa ggcccacggg gttctggcct gccagtcctg ccacgaatcc
     9241 atgcacgggc tgtacccggt gaggcaggaa ggtccggaaa agaccgtgga cttgacttcg
     9301 catcaacagg cccttcagtt cagcccggat aaccagtacg ccgggccggt cacctgcgca
     9361 gcctgccata cagtcaatcc caagggggtc cccgttcaac ttcggcagac cgaatacgag
     9421 gccgactatt gggcctccgt ggtcctcatt catttcatgc gtgaagacga tcagaaaatg
     9481 ccggtggccg aactaatcag aaaatacccc taccggaagg ccaaagaaat cgtcgtgaag
     9541 ggatggaagt aaattctcgg ttctcctcct ctagaggcat ctattagtta acccggaaag
     9601 ggcttccctt tttccttccc ctgaatgccg gcctttcagt ttaggaattt gaaaccagga
     9661 gggtgtcccc gaatttattc ggcgaatttt tctcttttcg tccggatcga aggaagcagc
     9721 gggaaaagga gaaacaacag agcaattaaa agagaagtca gcgccagagg tcgtgttaca
     9781 aagatgccca ggctgccttg ggaaataatc aaggactgcc taagcgccgt ctccagcagg
     9841 gggcccagaa taaaggctag gatgaaagga ccgccgtcgt actcatattt tttcatcaga
     9901 taaccgaaaa ttccgaagat cagcatgacc gccacgtcga tgaccctgag attcacgctg
     9961 taagcgccga tcaggcagaa taaaataatg agcgggaaaa gaattttgta ggggaccttc
    10021 aatatcttca cccagattcc gatcaagggg aggttcagga ccagaagcat gagattgccg
    10081 acgaccatgc tgaccatcac cccccagaaa aactcgggat gattctgaat gaggaggggc
    10141 cccggggtcg tcccgtggat catcaaggcc gccagaaaca gggccatcac gacgtttgag
    10201 ggaattccca gggtcagaag cggcaccatg gacccaaagg tcgccgcgtt gttagccgat
    10261 tccgggccgg cgaccccttc gatggccccc cggccaaact cttccggtcg ccgggatatc
    10321 cgtttttcaa tcgcatagga aacaaaggag gagatcactg cagttccccc cggaataatc
    10381 cctacaaaaa atccgatggc cgaacccctg gcgatcggtt ttatggacct cctccagtcc
    10441 tcacggttcg gcagaagccc ccgaatcttc gtttcataga tattcctctg ttcctgctgc
    10501 tcaagattga tcaggacttc ggatatcccg aaaaggccca tgaccacagg gaccatgtcc
    10561 agaccgtcca ggagagactt ggccccataa gtgagcctgg gcgtcccgga gatcgggtcc
    10621 gtgccgacgc atccgaggac gatccccaag gccaccatca gcagggcttt gatcatcgac
    10681 cccgagccca gaaaggtaga caaggtcagc cccaggacca tgaggccgaa aaattccggc
    10741 ggcccgaaat caaaggcaat gcgggccagg gacggggcca gtaccgaaat catggcaacg
    10801 ccaaaatacc cggcaatgaa ggagccaaag gccgcaatac ccagggccgg acccgcccgc
    10861 ccctggcggg ccatttggta tccgtcaaag caggtcacga ccgaagcggc ttcccccgga
    10921 atattgacca ggatggaggt ggtcgaaccc ccatacatgg cgccgtagta gatccccgcc
    10981 agcatgatga tggccgaaac cgagggcatg gtgaacgtgg ccggcatgag gatcgccact
    11041 gcacccgaag tcccgattcc gggcaaaacc ccgatcagcg tcccaatcag gaccccgata
    11101 aaacaatagg ccagattcat gggttggagg gcggtggcca ctcccatttg taaactgctt
    11161 aagaaatcca tcttctccta ccggatatat ttgggtaatc tccgtaaggc aaaccccgac
    11221 actgctgggg gtccgcctgt ggcggattgc catgttccgg gatgcttagg tcgtggcccg
    11281 ggggcaagga gcttctgtac aaaaagcatc ggccgggctg gccctcttct cggaggcttc
    11341 gaccagcttc cgcaagatgc ggcgggccga tctgtagagc gaccggtcgc ccttaccagg
    11401 aaagaaaacc ttccttggag gttcaaaagg cgggcccgcc ccgcccataa aaaggaagcc
    11461 gcctcatggt ttttgtacag aaggtccttg cctccgggcc tggcacccgg ccgaaggcca
    11521 tttttagata ttgccctttt caatccgctt taagcggttc acttttttta ccctcggctc
    11581 tgccggaggt cgtgattctt taccctgtga ttgccccgtt tggtttctgc gaaaagttta
    11641 cgcgaaaatg ggccgcagca ggccgggggg aaattgcagt tccaagaaaa cggaaaaaac
    11701 caggtaacag ataataatgg tcaccgccga caagaggaga gccatccccc acctctgggg
    11761 ttccaatccc ttgaaaagaa agatgagcag gaaaaaggtg ctcaatatga atcccagcca
    11821 gtgaaacacg gccacataaa ggaataaggc cagcatcagc tcgattccct tgggccaccg
    11881 ccttccttgc catctgattt tcttttcttc ctggacagcg gaaaagctct gaaggaaaaa
    11941 tgcggcgctg aggatcatca taatgatccc catccaaaat atcagaaacc cgggcttcgg
    12001 cgccctcggg gtgcccaatt tcagctgaac cccctcgaag gcaatgtaaa gaccgaagag
    12061 cccccagatc agggattgaa tttggtctgt tttcttcatc ttctttttca ggcacagatc
    12121 accgatttcc cggccaggag actagggata cctgcttcgc gatcctcccg ccctttttcg
    12181 ccggccgctg acttttattc cggttgaatc cctgcccgat ccgacacctt tttgaaataa
    12241 tcgttctgat ttttgatcca ggctttcgct tcctcagggc ctccgtggaa ctgctccaag
    12301 ccattctggg cgataaattt tttgaattct tcggtctcca tcgccttttt aaaggtttcg
    12361 agcaagaggg tcctcacttc cgggggaaga cccttgggga ccccaacccc catccagcgg
    12421 ctgatgatga tatcatatcc cagttccttg gctgtgggag tatcagggta gaggcgggtg
    12481 cgttttgcac tgagggaaac cagggccctg attttattcg cctccttcaa ggtgaccgcc
    12541 tcggaaattc cggccgaggc aaaatccacg tgcttccccg ctgctgccac cagagcaggg
    12601 gccgccccct tataaggaac gtagctgatc tgcgtgcctg tggcggcggc aaaagccgca
    12661 ttggccaggt gttgaggaga gccatggccg gcggttgctc cggtgacttt tccggggttg
    12721 gccttggctt tggccaccaa gtcggcggcc gtctttaaag ggctgtccgt ggccacggca
    12781 agaatcatgg ggtcttctgc agccacgctt agcgcttcaa gaagctcgac cgaaggatag
    12841 tctttgatca cgttgggggc aatggtattg gtggtcgtca gcagcatgag cgtgtagccg
    12901 tcgggcgccg cattgagcgc ttcccgtatc cccatgagac ccgaagcgcc ggtgatgttt
    12961 tccacataca ccctaccgcc caattgcgga ttgaccaagc gggtcagcat tcgggccatc
    13021 acatcacttc cccctcctgc cgcccaggcg cagataaact tgatctttcg ttcggggtat
    13081 ttcccccagg ccgattggat tcccagggcc aacgggacaa ctagcagcaa taaaaccacc
    13141 atcgcaagat ttcttttgct catggccgac tctcctctcg tgcttctttt tgagtcagaa
    13201 attcgttcca taaaatgtct gattaaaaac ttccttcata gctcaagcca gatattgaat
    13261 tttatagcac tttcatccag ggtttacaat tccctccccg atttccaaaa gagggaattg
    13321 ccattgaacg ccgtcccctt tttcccgtcc ctatgcgggc gtgtgctttc atgccctgaa
    13381 aaggggcgat cagatggttt agggttatgc tttcttgggc aatgtctctc ggagggaaag
    13441 gcggtaggct tattcggtca aggtaccctc atatccatta aatgacaagc ggagcaggca
    13501 ttcaccgagg gcttgtggga atggtgacat ttctcgcatt cgatctcgcc caggtgggag
    13561 tcgtggggat ttggattgat tttactggtt ttcgccgcca ccttcgagta atcgccatgg
    13621 catcccaggc agacctccat cggaaccttt tgcttcggcg ggtcttcccg gtggcagcct
    13681 gagcattcga tgcctaggtt cttgtgtctg tctcctagga aaaggtcttg ggcagccagg
    13741 gtaaggacgg atggcagaag aacggaacct ccccagccac acaagaaagc catcgcaggg
    13801 atcaaggtcc ggaattgcat gggggctcct gctggtttcc aatgtctcgc aaaattcagg
    13861 cccggggttg agtcccgctg taggccgcgc atcttcgaag gcgggtcggg gttaccggcc
    13921 cgccccgggt gcccccccgg gcaagagcga tgtcacgcca gcggtttttc cgccgaggca
    13981 ttccggcctg cgatgcggcc gaagaccgtg caggtggtcc aaccgcagcc tccgtctcgt
    14041 tcccatccct ggaggtttcc gatcatctcg cccgcggagt aaagccgggg gatgacccga
    14101 ttctgtcggt ccaggacctg ggcattgacg ttgatgcgga ccccgcccac actggcatgc
    14161 cggtaatagg tcttgggcat ggcatagaag ggcccggcct cgatcttttg ggtaagaaac
    14221 ttcttgaact ggccgaaatc ggcatcataa ttctgttcta caaacttatt gtagcgatcc
    14281 acggtggcca cgaggttcgc cgccgggacc ttgatgacct ccgccagctg cggcagggta
    14341 tccgccgcca gagcgctttt ggtttcgagg gcgcccttga gatatacctt gttggcagct
    14401 gcggaatcgc agatgcccca ccactggtag atgtcttttt cgtgcaaaat ggtgagccgg
    14461 gcctggcggt agacgctttg gtcgccgcct tccatccaga agcgattgcc ctggttgtcg
    14521 acgtcgatga aagtcccctc cttctccgcg atgagcgccg acccggggcc gctgtaagcg
    14581 accgctgatc ccgacatgcg cagctgctga atatgcagga gcgcggcgtc ggccccgatg
    14641 tccatggcag ccagaagaac ctctccggtg catcctatgc ctggctttcc ggtcgcttga
    14701 caggaggccc tgacagcggg attcaaccgg gggtcgtagc ggttacacat cttgccgttg
    14761 gccgtaaagc cgcccgcggc gaggaccacg gcccgcttgg cgcggatgcg gattaccttg
    14821 ttcgcagcaa tgtccttgac tctcacgccg agcacatcac ccgacaggcc gcccttccgg
    14881 atgatttcca ggacctccat ctgggtcaga atctgggccc cacgcttctt ggcatggtcg
    14941 gccagacaca tgaccccggc ttccccggcg agaaaatcct ggtattttcc cccggtgtgc
    15001 tgcctgcctt tggtgtaggg gccctgccac gcgccatcga cgtagggggt attccagagc
    15061 atgttcatgc gcatggccag ggggttctcg ggcatcggat tggggccgag gctgtggtaa
    15121 atcgcgatgc cgttatgggt aaatccgagg ttcgtcagcc agttgtaccc gtcgagggac
    15181 tgctcgacga agacgcgtac aagggccgca tcattcaggt atccgcccag caataatttt
    15241 tccttaatga agaggtccgc ggagtcgttg gcataccaat gggcatcctt ctccgcgccc
    15301 ttgcgctgtt cgggcgagtt gaaggtgccg tagttcccaa agctcatctt ggagttgccg
    15361 ccgatgcccg gcaatttttc gagaaccatc actttggcct tgctgtccgc cgcctcgacg
    15421 gccgccgcta gccccgacgc gcccgatccg acgatgacca catcggcctc ccggtcccat
    15481 ttccccgaag cggatcgggg gggcgtttgg gattcccctg ttgcgctcac cagggccacg
    15541 ccagcaatcg ctccgccggc aaccgtggca ccctttagga aacctctccg ggtgattgct
    15601 ttttgacccc tcatgtcttt catgcttttc tcctttccct ttcagcggct ggttagactt
    15661 tgggcttcca cagacgttct tctcccccca aaactatagt ttcaccacaa taatgaatat
    15721 aatcaccgcc catattgcta accccaacaa aatcccccaa gctagcccct tatgagcatt
    15781 aacaaattcc agataccgat ccatgcttct tctcaaatgg ccgacattac cctgcccctc
    15841 caggtttctc ctgtcccgcc cgcttctgcg ctcttggccg gttcgtcttt ccggcttacg
    15901 tttgcggata ggatccctcc gtctatcatg ccctaagcgc ctaccccctt tatcaactat
    15961 gcggctgacc caattagtat caggcaaggc tatcccctcc ttataaattt cccccaaaat
    16021 agcgaaatga atgaataggc cggagcgtgt attttagccg caaaatattt tttttctagt
    16081 ctaagagatt ctactgcaag ggttccactt gagatgttgg agtggaaggt cgaattgaaa
    16141 accaagactt tactgacggc cgtcttataa ttattttatt ataaatgcgg gaaaaatggc
    16201 aagaatcgat agcgcctcat ctcatcagaa acacgaccca agagaaaaaa ttcgggagcg
    16261 ttacgctcct cgggacatat gagccattca aggtcgaggc tccgattgtc ctaagggaag
    16321 gaatcggggg ggtatccagt cttatctgtg ttgcttccac tattattttt ttcgctgtat
    16381 taatcattgc taccatactc cggttttgtc tgagcaatat atagaattcc agagaatgat
    16441 ttgcggatca accgacggga ccttccccct ttttcattgc ttctaatttc ttgccgtttt
    16501 gtcaaaaaaa accaccattt tttcataatc tgaaatcttg tcaattagtt gggcttaaaa
    16561 aaaaatattt tgactttttg gcaagaacca tccctaactt cctggtacat gtcgggaatc
    16621 ggtgacgata aatttaaaat atttaaatat tacaatacct tataattatc gtccggaatg
    16681 gtggggcatg ccgctttggc actctgcttg ctcttgtaaa agcctatggg tgcgcttcaa
    16741 attgaaagga ttcggatgat cgtaaacggg gcggggaatt cgagcggttc caaccccctc
    16801 ttcctttcgg ctatggtaat gatgatgcgc aagagcgcct tttctgcaaa ggatatccga
    16861 aaaacatcct gcgataatcc tgagcgattt tatagaggca gcaccccatt ttgaactaat
    16921 ctttgaaatt cgtggggaaa aattatatgc aaagttcaag tcccgctttt cctctccagg
    16981 gcagcaccaa cctggagctc gcccgcttgc ccaatcttat caccgtcgcg gcagatgtcc
    17041 tcgcggggta ttggatcgca aacggattga agatggattc ccggcttctc tggctcctgc
    17101 ttgccacgtc ggctgtttac gctggaggat gcgcgctgaa cgattatttc gaccgggata
    17161 tagattcccc ggagtgcccg ggacagccga tccccagcgg aagggtggag ccctggaaag
    17221 ctttaattta ttccttatct ctcttcacgg ttggactcgg tgcttcggtc cttgccgggg
    17281 cgtctgcccc gcacataatg gttcttctga tccttgcggc tgttctttat aatgtgggga
    17341 caaaagaaag acccttgctt ggtcctttga atttggccgg ctaccgatcc ctgaatctta
    17401 tcttgggaat gggcttgccg ttctggcttg ggctttctgg gcttccgagt aggtactttc
    17461 cggtcttttc cttcctttac gtttttcttc ttagctggtt gagccgattc gaggtggggg
    17521 ggaaaggaag ggaaatcacc gccgtggctt tcttagggtg ggctaatttt gtcacgatcc
    17581 ttctgttcct ctcgtttggg gaggccatga atctggaaaa tttgcctttc ctcgctcttt
    17641 ttatggcgtt caccggcccg gctttgttga aggcaggccg cgatcagacc acctttgcgg
    17701 ccgggggcgc tctgaaaatg atggttctgg ggctccctct cctcaatgcg gtttactgct
    17761 cgggcgtcca gggtttcgcg gcgggagttc cggtcgccct ttgcacggct atcgcttctt
    17821 tattggccat tagcaaaaga gtttcctgtg acctgacgca gggattgggt tggggaagaa
    17881 actccttttg atccatgttt ggcaagcatc cggggcagca gtaaaaaagt tatttttcta
    17941 ccctctttcc ttcaggttgc tccccgtatc ttcaattcct ctgaccaatc ctacacctgt
    18001 taatttcctt aaggtaaacc cccggctctg ccccgggggt ccgcctgagg cgggttgata
    18061 tttccaggat gcttgggttg tggccatggg gcaaggagct tcttgacaaa aagcatcggc
    18121 taggttggcc ttcttctggg ggcatcgatc ggcttccgca agatgcggcg ggcggaacgg
    18181 gtccccgtac aacagaataa tcctttcctt ggggaaagaa gggttggccc gcctaaattt
    18241 ccctttccgt gccccccatc tgatacaatc cgggcctacc caagacttgg ttcaacattc
    18301 acttcgttga tgactccacc tgccaaatgt ggagaatagt caactccaag tatggtgaaa
    18361 ggtggtctgc cgaaaaaggt cgggcgacca ccacctccga tgctttggcg tcttcttcct
    18421 cctccctaag aaatcaaatt gggccgagaa ggttctgtgt tataatttca aaaaatggta
    18481 ataatcaacc aaggccagga atatccggaa aggagccaat tggataagaa agaggaagga
    18541 tcggccccat gaataagagc ggcataaagg accttctgtg cctgcttctg gtatgtcttc
    18601 cggttatttt atgcagtcag gccggggaat ctagcccgtc ctcaccctac ctattttcag
    18661 accaggagct cgataacctt cttgcgccta tagcgctcta cccggacccg ctgctcgctg
    18721 agatcctgcc agcgtcgacc tatccggaag aggtagccga tgcggcggcg tggctgacaa
    18781 gcggcgggga tccgtcaggg atcgatgaga agaactggga tgaggccgtg agagcagtgg
    18841 cccattaccc aaacattctt tttatgatga gcgggaatat ggattggacc gccgacctgg
    18901 gggatgcctt tctcaaccag ccggaagcgg tggccaggtc aatccagcgc ctccggtggc
    18961 gggcgaaagc cgtcgggaat ctgatcagta acaatgaaca aacggtcgtt attgaaggcg
    19021 actatattca gatcatcccg gctcaacctc agtatattta cgtcccccgg tatgatcctg
    19081 cggttgtata tgtgcaaaga ccggccgaag ggatttcgcc tttcattacc tttgggtttg
    19141 ggctggtaat cgggggctgg ctgagcatgg actttgactg gtataaccac catatcatct
    19201 accatggctg gaaccgccac ggctgggtta ataatgcgag gccctacgtt cacataacga
    19261 acgtttatgt caatagaagc aggccttaca tccatcagac ctggaagcat gacgcctccc
    19321 atggggaccc ggatcgctac agggcatcac ggcccggcgg cccttaccgc ggcaggtatg
    19381 cgcgcacgcc tgatgcgagg gggagagcta caacagcgcc caggccctca ggagggatgt
    19441 tcggctacaa ggccgatgcc ccagcatcca gcaatcgcgg cagggatagt ctcgccgtca
    19501 ttcggccaca gatgccaacg ccgggtccgg gtgttgggca agcgccgcca aggccaactc
    19561 cgggcgtcag ccaaaagccc acagccccga ctccaagcac cagccaaagg caagtaacgc
    19621 cggctccaag gacaagcagc ggcatctctc gccccagttc gggcccaggg agcgcgcagc
    19681 tggccaggac accttcaagc gcctttggtg gctacagggg agccaatgag gccagaaccc
    19741 agagcctccg cgggcaggcc agccgccaga gcagtgtagg agcacctcct cccccttccc
    19801 ctgcaagtcg gggcagtggt cctgcgggca aggccacccc caggggcaag taaaggtccc
    19861 atcgatagcc gggcggtagc ctgaatattt cctgggccca cggaatataa gtgaattatg
    19921 aaaggaaaga aattatggag acagttcaac ggaaactccc attttttcca tcgcggagta
    19981 tatcttttat cgttgcgctc ctggccgccg caaccctggt ctttgcctgg agcgcagggg
    20041 aagcaggggc tgcatcgcag agactctttt ccactccgga ggatgccctg aaagagctcc
    20101 atggagctgc ccaggctaag gacagggccg ccatggacca aatattcggt tcctcttaca
    20161 aagaccttct ctccggcgac gaggtgcagg atgctgccga atttgcggaa tttgtcgaac
    20221 acctggcaga gaaaaccaac ctcgttaaag agagtgattc aaaagtgacc ctttacatag
    20281 gcaacgagaa ctggcccttt cccattccca tttcaaaaaa ggacgaccaa tggttctttg
    20341 atacggaagc gggccgggaa gagatcctga accggcgggt cggggaagac gaactcatcg
    20401 ccatactggt ctgtcgcagc tatgtcaagg cgcagcgcga gtacgtgctg aaggattggg
    20461 agggcgatgg ggtggttaag tatgcccaga aacttcggag cgacccaggg aagaagaacg
    20521 gtctcttctg gaggagccgg ccgggcgaag aagtgagccc ccttggagag cttgtggccc
    20581 aggcgagaat ggagggctac cggaagacaa aggcggtctt cagggaagag ccgacgccgt
    20641 ttcacggcta ctattttaag gtactgacaa ggcagggaaa gaatgcgccg gggggaggat
    20701 acaattacgt catcaatggc aacatggtcg cgggcttcgc cttagtcgcc tctccctcga
    20761 attggggcaa gtccggggtt atgactttta tcgtcaatca gcaagggaaa gtctaccaga
    20821 agaacctggg ggcgaatacc ttgaagatcg cccaggcgat ggaggagttc aaccctgaca
    20881 agacctggac gctggtgaag gaatgatgat cgctcccttt gccgcttttc gtttgaaata
    20941 ttgaatcgca ggaagaactc ggggcaagtt acacccttgt gagcccgagg gatgcggcaa
    21001 tctcactctt gaatgggatt tcttcgtctc ttcgttgcac gcaaccgcag gtttatattt
    21061 tatatggatt cccaggggct ataaaaggag gtctgagaca tctccccccg gggatacaaa
    21121 tagaaaaggg ataattcggt tcgtagtccc gattttcgtt gcgcatcaat cgaaaaggag
    21181 gttagtctct tctttgactc caacgagaga ttcgataacc acaaacctcc ttatccctgt
    21241 ccgaaaagta aaatagaaca ccccgcgcct ttaacataaa ggcgagaaca aaatctctcg
    21301 cctttcaatt caaaccccgt tctaccactc tcatttcctt tcaataattt agatgaattt
    21361 ttcccaaaga agattccaga aattcgactc gggccactta ggaaacctct tcttgcgaat
    21421 ctcgttcccc ggtaaatctt ggagaaaatt gcttggggag gaatcttaga tttcaaaccc
    21481 aggtcgagga tttgtattta aatttcctct tttgcccagt tattctccgt cattatcaac
    21541 atgcgcgccc ggcgcactaa catgccatac ccgaaacctc ccaatttgcc cctattgttg
    21601 gctaaatggt ttagatatca aagcttttta agcccccgga atcctcaatg ccaaaaacag
    21661 gctcttggca attatcgaaa ttttgtattt cctctgaata aaacccttca cctcctcttc
    21721 ttatcccgga tataattgat gtaggcaaaa aacggagaaa attcggggaa aattcattga
    21781 aaagatcggt tgtcgccatt ctaaaaacga ctccgcaaag aatcttcgat gatattttgc
    21841 gagtcatggg aatggccgag ttcaaagacg ccttgaaggg ggaaaccgga accatcctaa
    21901 aggacaatat ttcctggcac tttcccttcc tgtcggcgaa tacgaccccc tggcagttgg
    21961 agggcaccat tcgcgcactt caggcggctg ggtttttgaa tctcgtggcg gtccacaata
    22021 agaccgtcgt aaccaatgcc ttcaaaggtt tgcgtttgaa taaattggcg ccgatctacc
    22081 gcaaatatag gatcccggaa aaatttaatt ttttgcccca ggacatgacc tgggaggtct
    22141 tccgtcccaa ggcaagaatg aaggttttgg accggattta tccgaaaggg ataaaggtgc
    22201 cgaccttttt tgcggggaaa aacatccttc atctgcccac ggcgaaatgc catatatatg
    22261 ccacggttac tgggtccatg aaaaatgcct ttggggggct cctcacgacc cgacgccatt
    22321 atacccacag tgtcatccac gaaacattgg tagatctttt ggccattcag aaggagattc
    22381 acccggggat cttcaccgtc atggacgcga ccgtctgtgg aaacggcccc ggccctcgga
    22441 ccatgatccc tgtagagaaa ggattgatcc tcgccagcgc cgattctgtg gcaatcgatg
    22501 cggtaatggc gaagatcatg ggttttgacc cgatgaagat cggttatatc aggctcgctc
    22561 acgaagaagg gttgggcatc ggaagggtgg aagagatcga ggttcgtgga gaggacatgg
    22621 gggggatgaa tttccatttt tccgtcggaa acaacttggc cagccgattt ggaaatctat
    22681 tctggtatgg cccgttgaaa agactgcaaa aggttttctt ccataccccc ttggtaaatc
    22741 tgtttgtcat ggcttcttat ctttatcatg atcgattctg gtggccggtg aaggggaaac
    22801 gcataatgga agatgtcaag gccggcaccg cttggggaaa attctttgcc ggctacacac
    22861 ctatggaaat tgagaaacct tagtttttta agcggttctt tcggactctg acggatggaa
    22921 gagacgcctt ttattgaaaa agatagctcg ttgcttcgga aggcagcgtt ctttccactg
    22981 gtggtggacc tggatggaac cctggcaaaa acggacctgc tcattgaatc tcttttttcc
    23041 ttgataaagc ggaacccttt ttatgttttg gtccttcctt tctggctgct caaggggagg
    23101 gcctttttca agcggcaaat caatcaacgc gtcaccttgg atgtcaacag cttgccttat
    23161 gaccgcgagt ttctcgatta tttgaagggc gaacgtagcc gggggcgtcc cttgttcttg
    23221 gcaacgggaa acgatgaacg aatcgcccgt caggtagccg aacacttggg actatttgat
    23281 cgggttctgg ccagcgacgg agtcgtgaac ctttcttcga ggaccaaacg agaccgattg
    23341 gtcgccgagt tcggggaaaa aggcttcgat tatgcgggga atgaaaagcg ggaccttgaa
    23401 gtttggcctt cggcacgcaa agcactcatc gtaaattcta ccaaaagtat tttgcggcga
    23461 gcgggtcggg tagcggatgt cgaaaaggtc ttccatagga gggacggggc cttaaaatct
    23521 tacctaacgg ccttacggct gcagcagtgg cccaaaaatc ttctggtctt tgtccccctg
    23581 gtcatggccc accgttataa cgagttcgcc ttgatcggca acgcgttttt agctttttgc
    23641 gcttttggat tatgcgcctc cagcgtttat atcataaacg acctgactga tctctctgcg
    23701 gaccgacatc atccccgcaa gcggctgcgc ccttttgcgg cgggagagct ctcccttctc
    23761 tgggggttgg gatcggctcc cttgttgctg gggggcagca tgcttttggc ctggtttctc
    23821 cccttgccat tcctcggaat gctgattcta tactttttat tgaacctggg ttattccttt
    23881 actataaaaa gggtaatgct attggatgtg attaccctcg ccggactcta tgccatgaga
    23941 atcatggctg gttcggcttc ggtcgccatt tggccatcag cctggctcct ggccttctcg
    24001 acgttccttt tcctgagtct ggctctgctt aaacgctatg cggagctggt catgacccat
    24061 cggatgagcg ggaggaatgt ccaagttcga ggctaccagg ttattgataa ggaacttctc
    24121 gtgtccctgg ggaccggcag cgggtatgtg gccgttctcg tcctagccat gtatatatcg
    24181 agcggtacgg cagaaatcct ttatgcccgt catgagctca tttggttttt gtgcccccta
    24241 ttgctttatt ggatcagcta tgtttggctg atcgctcatc ggggcgatat ggttgacgac
    24301 cccctggtgt tcgtgttccg gaaccaggtc agccggatcg tcatcctgct aggcgcattt
    24361 atcgtcttgc ttgcgaggta ggcatccgag gtgaaatccg aatttcaaaa agccacaaat
    24421 cttagccaaa ttaatacaaa cgcattaaat aaaatgacat cacccccacc cttaccctcc
    24481 ccccttgagg gggaggggag ggtgggaggt aaaataatat atttcgtttg tattagtcgg
    24541 aggaaggtat ctccagcaga agacccccca tttgattcct cccgggtccg cttgggactg
    24601 gttgtctttt ctcttgcctt cattactatg ctgatcatat ccgcactctg gaatcccgaa
    24661 ggaagggcgg atgacccggg ggtctccatc cctaaaacca taatagaact gcagcccttt
    24721 cgtcagacga attcgatcca gatcaaaagc gcacagggcc gggagggcct tgcgactctc
    24781 attaacatca atcccggcgt caatgcctgg tttttgctca acctcgattt tcatgaagga
    24841 acgccagccg aagaatatca tctgcaaaat acgaaccccg aaacgcagaa gctatctctc
    24901 gatgccgatt atccttacgg tcttgtcgtc gaagaagggg taaacaggta tacctgtaat
    24961 ctttggggag tggagccccg gacggtgctg aaaagagcca gggcatcagg cgccgcctat
    25021 gctcctcttt gcgaggggag actgtacttg cggaaccccg ccaagggaca tcggaccaga
    25081 atagaaatgg cgaccgactt tctgcgcgat gaggtaccgg ggggcgagaa aattgtcacc
    25141 attgtgaggg atacattttt cgccgatgct taccgggaaa agacgaaaac ggctgagacg
    25201 ccgaaaactt atgccaagga actatccccc gggaaaccgg gaaccgtccc ggcatcggcg
    25261 ttgctggatc aaaaagaggc tgggcgtatt gtcgtatcta cccatttagg tattgaagtc
    25321 ctggaatcgc caccccatga aatcgtcctg ggaaactggt atgaggctaa ggacaatcct
    25381 ggcatcttta tcagtctgat cgcggccaat cggatcgccc cggagattct gcgaagctac
    25441 ccgaagaggg tcgccaattt ggacaggctt gaagcggagg ccctggttta catgattgct
    25501 ttcgacctgg accgattcaa cctgggattc gccctgggaa cagaacatcc ccgagtcgat
    25561 tggtctgatc acgcgcgcga gcagatgaag gatccctccc ttcccggacc ggacgggatc
    25621 ggaaatattg ttcccttggt ttcgaccgga ctcattaacc caaaggacgc gggcaggacg
    25681 gttgccgcct ttacgggtgg attcaagcgc gcccatgggg cgtttaggtc gggagatttg
    25741 gctttgaaaa accatggaag ccactatggt tttatggaaa atggcgtgat attcagcaag
    25801 cttcaacccg ggctcgccac catttatgtg ctcaatgacc gccgggtcga tatgaagacc
    25861 tgggaggatg aggacaatcg gctccttccc agcatccgat acgcccgcca gaacggggtc
    25921 cccatcatta cggactttga tcctgcgagc caaatgtctg tgccaggccc cctcgtttcg
    25981 cgatggggag aaggaaattg gtccggatcg gaagacaata agcttaggac catgcgcgca
    26041 ggagccgctt tgcaggaaag gggagaaaaa cgattttttt tgtacgcagt tttcacgagc
    26101 gcaacaccct cggcaatggc ccgcgtcttc cagggttacg gggcccgata cgccatgctc
    26161 ttggacatga atgcccttga acacacctac ctgtccgtct acagacggca gggatcgaga
    26221 ctttacgtgc agcatctcat ccaggggatg agcgaagtgg acaaatcctc aaagggcgag
    26281 tatattcctc gcttcctcgg ttttccggat aatcgggact ttttttactt tttgcagaaa
    26341 gaggcgccat gagagagttt tttgtttcag gcgggatcct ggcatttttg tggctggcgg
    26401 cccttttgct tcctttccct tcctcggccc agcctcttaa agagaagaat gatgccctat
    26461 tcgaacagct ccaaagagtc cacgggctct ccgatgatca aatgcaatcc atccgccgaa
    26521 ttttttctga atccggctat atcggccagg gcaatccggc tataacccgt cacccggtga
    26581 ctccgcagca gtgccaggaa aaactcaccc tgcttggaat ccggtatgag aacgaagaat
    26641 tcgaaaaatt ctgcggggcc cgatacatgg ccccccttta caatccggcc ctggaaaatg
    26701 ccaaagacgc aagcgtctgc attgatcagt ttgagtttcc cgacattccc tgcgtctacc
    26761 cggtcgtctg ggtgcgcgcc cgggaagccg cggaaatatg ctgggccatg ggcaaacgcc
    26821 tctgcgacgc ccacgaatgg gaaggggcct gtgccggagc gctcgaaacg gccgattacc
    26881 ggttcgatct ggctcaaggg cttcctccca acacggcagt ggaaaggatg cgccaagctc
    26941 ataataaagt gtatggaacc aggaaagtct gggcctatgg ttcccactat gagaaaggaa
    27001 tatgcgccag ttcaagccaa aaaagcccct cttgtggcgg gggaagttgg acccaatgcg
    27061 gatccaatac cttccctgca ggagactttc ccgattgtca tagtcgttta caggtctatg
    27121 accagcatgg aaacgccgcc gaacatatga accttccgtt gaatgaaggc cagatgtcaa
    27181 gccggggcag taaggaactc ggctataccg agatgaaggg cagttggttc attttcgaca
    27241 cctactatgc ccacgaagat tggtgccgct ggcgggcacc tttttggcat ggaagccggg
    27301 taatggatag gcacagccat gagaactacc atcttgggtt ccgctgctgc aagacggtca
    27361 aggggaacta gaactcggcc ttgactttaa tgtcccataa agattcgtgg tgtccgggtt
    27421 aatgcttgat cgaacggtag ggaggttcga gggatgaaga gattgccgcg gggtcccttg
    27481 gttgccaaat atatcgcgcc atgaaatctc gaaccttggg gatcgctccc tcgtcgctta
    27541 gcacggcggc aagatcggga tcttccaaaa tcctgaaaat gggtgtaggt tccccgttta
    27601 gcaaggccag ttcgctgatc aaacggaccc cgtgactgta atcggcatac cctgcgccgt
    27661 gggtcgtaca aagaggctga atcggagtcc ccgaaagatg ctgccatcca taaatggcga
    27721 ctctttccgg tttatgggcc agctgattgg taatcacaat atctttttta tggccagata
    27781 ttaacgcccc caatggacag cccagagcca aacgttgttc ttggatcttt tgattatgct
    27841 ccacgtaaca ggccgtcgaa gtcatccggg ggccgggcgg cataggctcc ggcctcagat
    27901 ggaaggctga ctgtttgaat agggcgtcaa ccatcttcgt tgttggcagg ataaagccga
    27961 atctgtcggc tattgccgct gcggtgtaga gattcattgg agtccggata aaatcccgat
    28021 tcgaccccac ccctaaatag tccggcattg caaagatggt cgcagtgatt ccttttccgt
    28081 ctttaagaat gtgttcaagg ggaacgggga ttagtttcct caggaaatcg gggaaattgc
    28141 cttggctcaa ttgctccagg atagcctgtt ctcgccgggt tttatccatt ttggcgatat
    28201 atttggcaaa ctcggacccg gccattgccc aataggggcg gaagggaatt cttcggatca
    28261 aaaaatcctg gaagtcggcc cactctgcgc cgaaggcggc caaagaagga aagcaaatag
    28321 caaaaatgag agagat
//
LOCUS       DASPRR010000136         6273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1810637, whole genome shotgun sequence.
ACCESSION   DASPRR010000136 DASPRR010000000
VERSION     DASPRR010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810637"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /gene="tig"
                     /locus_tag="VEL68_05050"
     CDS             complement(<1..1284)
                     /gene="tig"
                     /locus_tag="VEL68_05050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXZ35367.1"
                     /translation="MKVQIENKGPYEKKILFEIPKEVVSQEVESHYKALNRNVKLKGF
                     RPGRVPRSILERYYRTQVETEVVSKLIEDSYEKAVEEYHLSPVASPQVLDRIFEAGQD
                     FKYTVTVEVKPEVSVEGYRGLEVEKPAVGVTEEEVEARLKMVQDSHAQLKPVEIDRPL
                     QEKDFTVIDFEGRLDEKPLEGWKAKDHLVEVGSKTMVGGLDQKLVGLPLQKDHDLVIT
                     LPEDYPRKELAGKEIKVRVRAKEIKEKILPALDDELAKEAGDFQTLQELKDRLRKNLE
                     EQKKAQAEQTAKERLLDLLREKHPFPVPKSMIDRQVETILARTESRLAGQGMKFDSAN
                     LDPQKLRESLTPSAEKEVRGSLILEKVAEMETITASEADLQQRLEQLAAQMNQRVEAV
                     KSQLQKKDRLEDLRAVIQEEKTLDFLLSQAKIVEGK"
     gene            1783..2325
                     /locus_tag="VEL68_05055"
     CDS             1783..2325
                     /locus_tag="VEL68_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXZ35368.1"
                     /translation="MKIPNPEECLLLLQKYGVPPNVVEHSRKVHRVAMFLCRELNRQD
                     EGLDPGLVEAASLLHDIAKVASFESGENHSQAGARLLHEEGFGEVAEIVRQHVILDSG
                     VDHGRITEAALVNYADKRVQHTRVVSLGERFQDLRERYGKTPEARAWLDSLEGQNRVL
                     EARIFRKIPFVPEGLAEIPG"
     gene            2602..2931
                     /gene="bamE"
                     /locus_tag="VEL68_05060"
     CDS             2602..2931
                     /gene="bamE"
                     /locus_tag="VEL68_05060"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HXZ35369.1"
                     /translation="MKFWMLLLIPVIAFSIGCASTMMGTPIDKAKLEQLVPGTTPEAK
                     VTELFGEPFKKEMVSGDMTKYTYTYYEEHPRIFRKNVQTKQLLDVYTKGGVVQKYDLR
                     KEGIGDK"
     gene            3297..4502
                     /locus_tag="VEL68_05065"
     CDS             3297..4502
                     /locus_tag="VEL68_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ35370.1"
                     /translation="MTTYLIIGNGVAGNSAAETIRRTDPAGKVLIFSKEKYYFYYTPA
                     LPEYLGGEKQVKDFAIHDSQWYERNRIDLFLETEVTQIDSQKKIVQTPKGAKFPYDQL
                     LLACGGRSFVPPIPGSSSPGVFTLRTIEDANAIRKKFQEARKAVVIGGGLLGLEAGNG
                     LRKAGLEVSVVEFFPRLLPRQTDIAGAALLQRQMEEMGFRFYLGAKTQEIAPDKDGLR
                     VTLEGGPELSAQLVLISAGVRPELTLAQTLKLAVDKGIKVDDHMFTGLKGVYAAGDLI
                     EHRGRFYGIWPASMEQGRVAGANMAGQERTYAGTVPSNSLKVAGIDLLAAGELDVEGK
                     MDSVVVKDEARKIYRKLVLKDHRIMGAMLLGDTRGKEEVQRAIQSKKDISPWEKELSS
                     DKFDFSKLK"
     gene            4657..5277
                     /locus_tag="VEL68_05070"
     CDS             4657..5277
                     /locus_tag="VEL68_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXZ35371.1"
                     /translation="MVQVELADPKAELLREVLVSYLSDLRMEIAYSQQRELRDFLKKR
                     GDQLEELLQMVERELARKGRNMVNIDRLRRVDVLLGLTDWELKVVAQFLREQAVPEGV
                     TLFEEGQKADRLFILEEGEISVRLPRGESYEIQTPGKIIGWSFLIPPNRYTASGRTLV
                     ASKLLVMQSPDFYYLIHKEPRMGVKVMGNLAQVVAGRLLQWAGQQG"
     gene            complement(5434..>6273)
                     /locus_tag="VEL68_05075"
     CDS             complement(5434..>6273)
                     /locus_tag="VEL68_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HXZ35372.1"
                     /translation="AVWASEIKRHLQRIRQLSPRVFQGQFAGAVGTLASIGAPGFKLQ
                     KLMFADLSLAVPEIAWHVARDTMAEIVFVYAMIGSTLAQVCNEIIGLQRTEVAEVEEP
                     FHMGKVGSSTMPHKRNPMMSEGVMALSKLIHSQVGPALVDMIAENERDQRGWMAEWSF
                     LPETCCLLSGMLSWTNKVLAGLIVYPENMTRNLDALHGLLLSENVMLKLGGKIGRQAS
                     HEVIYELCMEAFEKRVSLKSLLLKDKRVNKYLTEKQIDDLLDPTKYTGLAAKFVDRVF
                     ERK"
BASE COUNT         1386 a         1724 c         1760 g         1403 t
ORIGIN      
        1 tttcccctcg acgattttgg cctggctcag gaggaaatcc agagtttttt cctcctgaat
       61 caccgcccgg aggtcctcca ggcgatcttt cttctgcaat tggctcttca ctgcttcgac
      121 ccgctgattc atctgggcgg cgagctgctc gagcctctgc tgcaggtcgg cttcgctggc
      181 ggtaatggtt tccatttccg ccactttctc cagaatcaaa ctccctcgca cctctttttc
      241 cgctgaaggg gtaagggatt cacggagttt ctgggggtcc agattggctg aatcgaattt
      301 catcccctgc ccggccagcc gggattccgt ccgggccagg attgtctcca cctgccggtc
      361 gatcatgctt ttagggacgg ggaaggggtg tttctcccgg agaaggtcca aaagcctctc
      421 tttggcggtc tgctcagcct gggccttttt ctgttcttcc aggttcttgc ggaggcggtc
      481 cttcaattcc tgcaaagtct gaaaatctcc tgcttctttg gccagctcgt catccagggc
      541 gggaagaatc ttctccttga tttctttggc ccgaacccgg accttgatct cttttcccgc
      601 caattccttc cggggatagt cttccggcag ggtgatcacc aggtcgtgat ccttttggag
      661 gggcaggccg accaatttct ggtccaggcc tcccaccatg gttttcgacc cgacttccac
      721 caggtgatcc ttcgctttcc atccttccag gggtttttcg tcgagtctcc cctcaaagtc
      781 gatcaccgta aagtcctttt cctggagggg acgatcgatt tccactggct tcagttgagc
      841 gtgggaatcc tggaccatct tcaatcgggc ttccacctcc tcctcggtaa ccccgaccgc
      901 aggcttttcc acctccaacc cccgatatcc ctccacggag acctcgggct tgacctccac
      961 ggtcaccgtg tatttgaaat cctggccggc ctcaaagata cggtccagga cctggggcga
     1021 ggccaccgga gagaggtggt actcctccac cgctttttcg taggagtctt cgatcagttt
     1081 gctgaccact tcggtttcga cctgagtccg gtaatagcgc tccaaaatgg agcgcggaac
     1141 tctacccgga cggaaaccct tcaacttcac attgcgattc aaggccttat aatgggattc
     1201 gacctcttgg gaaaccactt ccttggggat ttcgaagagg atcttttttt cataagggcc
     1261 cttgttttca atctgcacct tcatcggcac ctcggaaagg aaatgaaatt caagcttctg
     1321 ttctgggatt tgacataccg cacttatttc taaattaaca aataaccgca gattcgtcaa
     1381 tgtcccggaa tttcttgccc atcttcactt ttggccaagc aggcgtaaga tcgattgaat
     1441 ttcaggcatt gcccggcccg gaagctaggc gttgcgcgca aaaatcatga ggcgcgcctc
     1501 ctttcttgga ccgggcgggc aagccacttt gtcccaaaga aaaggggaaa gtcgatgtag
     1561 atgcgtcacg ccaaacgcat gaccgggtgc ccgatgcgcc cgccgcatcc agcggaaccc
     1621 ctgtcagacc tccgaatgga tattttcccc cgccgatgct ttttgcgcgc aacgccttgc
     1681 ttccgggccg caagcttgct tttcagcaga aaaccgcaaa agatcccctc acattttccg
     1741 ttgatccggc aatctttccg gtgctatgat gaatcccgaa acatgaagat tcccaatccc
     1801 gaagaatgtc tcctcctgct gcaaaaatac ggggtcccgc cgaacgtcgt cgaacacagt
     1861 cggaaagtcc accgggtggc catgtttttg tgccgagaat tgaaccgtca ggacgagggg
     1921 ttagaccctg gacttgtgga agccgcttcc ctcctgcatg atatcgccaa ggtggccagt
     1981 tttgaatccg gggagaacca ttcccaggca ggggcccgtc tcctgcacga agaaggtttt
     2041 ggcgaagtgg ccgagattgt gcgccagcat gtaatcttgg actccggggt ggatcacggc
     2101 aggattaccg aagcggccct ggtcaactat gccgacaagc gggttcaaca cacccgggtg
     2161 gtctcgctgg gggagcggtt tcaggacctc cgggagcggt acgggaaaac ccccgaagcc
     2221 cgagcctggc tggactcgct ggaggggcag aaccgcgtcc tggaagcgag gatcttccga
     2281 aaaattccgt ttgtgccgga aggcttggct gagattcccg gctgaaaaga aggacttggc
     2341 cccagcatgc ccaagaagca tcggtagggc gcgcggctgc gcgtccgtgc agaacgatgc
     2401 caagctgggg cgaccacacc gggtcgcccc tacaactcct ctttcgtctc ctgtggagac
     2461 ccgaaataaa gggaaaatac tgttttcagg ggactaaaat acagagttca ccccatttat
     2521 ctattttccg tttgaaaata taattttcta taattttggg gcggagttca aaaaccatat
     2581 tcaaagacaa aggaggaaaa aatgaaattc tggatgcttc ttctgatacc ggtcattgcc
     2641 ttttccatcg gctgcgcttc aactatgatg gggactccca ttgacaaagc caaactggaa
     2701 cagctcgttc cagggacgac tccggaggca aaggtgacag agctcttcgg cgagcccttt
     2761 aaaaaagaga tggtctccgg cgatatgacc aaatatacct atacctacta cgaagagcac
     2821 cctcgcatct ttagaaagaa cgtgcagacc aaacagctcc tggatgttta caccaagggc
     2881 ggcgtagtcc agaagtatga cctgagaaaa gaaggtatag gggataaata atcgggccct
     2941 gggaatcagg cattccctta tcaagccggg gagtttttgg ggataaagtg atgatggccg
     3001 gaagatgact tcttcgggtg tttgagtctg cttccgacgt ctgaaccaaa tccctttgga
     3061 gaaagatacc ccatggaagg tttcctccat ggggtatcct acttttggct tgaagccttg
     3121 attctcctct ttcccttagg agatggggtc gtaatgggaa aaacgccgac attcccgtga
     3181 gagcgagaat ccaggtccgg gaaatgattg aggtgtacaa ctgactggat gtccgtctcc
     3241 gcgggcataa caaattgtga taaagcctct agaggagggg gaaagcagag gcagacatga
     3301 cgacctacct gattattggg aatggagtgg cgggcaattc cgccgccgag accatccgca
     3361 ggaccgaccc ggcgggaaag gtcctgatct tttccaaaga aaaatattat ttttattaca
     3421 cccccgccct ccccgagtat ctggggggag aaaaacaggt gaaggatttc gccatccacg
     3481 actcccagtg gtatgaaagg aatcggatcg acctgttcct ggagacggaa gtcactcaga
     3541 tcgactccca gaagaagatc gttcagaccc ccaaaggggc taagtttccc tacgatcagc
     3601 tcctcctggc ctgcggggga aggtccttcg tgcctccgat tcccggctcc tcttccccgg
     3661 gagtcttcac tctgcgaacc attgaggacg ccaacgccat ccgaaaaaag ttccaagagg
     3721 cgaggaaagc cgtggtcatc gggggcgggc ttctggggtt ggaggcgggc aacggcctca
     3781 gaaaagcggg gctggaggtc tccgtggtgg aattttttcc ccggctcttg ccgaggcaga
     3841 cagacattgc cggggcggcc ctgctgcagc ggcagatgga agagatgggg ttccgtttct
     3901 acctgggggc taagacccaa gagatcgccc cggacaagga cgggctccgg gttaccctgg
     3961 aaggaggacc tgaactttct gcccaactgg ttttgatctc cgccggagtg cgacccgaac
     4021 tcaccctggc ccagaccctg aagctggccg tggacaaggg aatcaaggtg gatgaccaca
     4081 tgttcaccgg actgaagggg gtctacgctg ccggggacct catcgaacac cggggacgtt
     4141 tctacggcat ctggcctgca tccatggagc aggggagggt ggccggagcc aacatggccg
     4201 gccaggaaag aacctacgcg ggaacggttc cgtccaattc tctcaaggtg gcgggcatag
     4261 accttctggc cgccggggaa ctggatgttg aagggaagat ggattccgtg gtggtgaagg
     4321 atgaggcccg aaagatttac cgtaagctgg tcttgaaaga ccacaggatt atgggagcga
     4381 tgttactggg cgatacccgg ggaaaagaag aggtccaaag ggccattcag tccaaaaagg
     4441 atatctctcc ctgggagaag gaattgtcga gcgacaagtt tgatttctcg aaattgaagt
     4501 agcggccttt tccttttttc catttgactc tccccatcga atttagtaca ttcctttcga
     4561 aagggaagac ttgagagaac ccttgagtct tctccctgcg aaccttcact cttctaaacc
     4621 aggcacggtt gaacctcacc ttccaggggg acacacatgg ttcaggtaga actggctgac
     4681 ccaaaggcgg aattgcttcg ggaagtcctg gtgagttatc tttccgacct acgaatggag
     4741 atcgcctaca gtcaacagag agagctccgg gattttctaa agaagcgggg ggatcaactg
     4801 gaagagcttc tccagatggt ggagagggag ctggcccgga aaggccggaa tatggtgaac
     4861 atcgaccggc ttcgcagggt ggatgtgctt ctggggttga ccgattggga gttgaaagtc
     4921 gtggcccagt ttttgaggga gcaagcggta cccgaagggg tgacgctctt cgaagagggg
     4981 cagaaagcgg accgcctgtt catcctggaa gaaggtgaaa tttccgtccg tcttcccagg
     5041 ggggagagct atgaaatcca aaccccggga aaaatcatcg ggtggtcctt cctgattcct
     5101 cccaaccggt acaccgcctc gggacgaacc ctagttgcct ccaagttgtt ggtcatgcaa
     5161 agcccggact tctattatct catccacaag gaacccagaa tgggagtgaa ggtcatgggc
     5221 aacctcgccc aggtggtggc cggcaggctg cttcagtggg ccggacaaca gggataagag
     5281 tctccccctt ttcccaaccc agaggtcgat tccattcggg tttcggggtc gatgccccgc
     5341 ggatttaaga gaaattgaag gccaaatgcg gaagatgggc tgggcagcaa gaaaagaccg
     5401 gagacacccg aaggcgcccc cggcggccca aggttacttc ctttcgaaga cccggtccac
     5461 gaacttcgcc gccaaacccg tatatttcgt cgggtcaagc aagtcgtcga tctgcttttc
     5521 ggtcaaatac ttgttcaccc gtttgtcctt caagaggagc gatttcagcg aaacccgctt
     5581 ctcgaaagct tccatgcata actcatagat cacttcatgg gaagcctgcc ggccgatttt
     5641 ccctcctaat ttcagcatga cattttcgct cagcaggagg ccgtggaggg cgtccaggtt
     5701 ccgggtcatg ttctccgggt agacgatcag cccggccagg accttattag tccaggagag
     5761 catgccgctg agcagacagc aggtctcggg caagaaggac cactcggcca tccagccccg
     5821 ctggtctctc tcgttctcgg cgatcatgtc caccagggcc ggccccacct gggaatggat
     5881 cagcttgctg agagccatga ccccctcgga catcatgggg ttgcgcttgt ggggcatggt
     5941 gctggagccg acttttccca tgtggaaggg ctcttccact tcggccacct cggtccgctg
     6001 aaggccgatg atctcgttgc agacttgggc cagggtagac ccgatcatgg catagacgaa
     6061 gacgatctcg gccatggtgt cccgggccac gtgccaggca atctccggta cagcgaggct
     6121 caggtcggcg aacatgagct tctgcagctt gaaccccggg gcgccgatgg aggccagcgt
     6181 tcccactgcc ccggcgaatt ggccctggaa tacgcggggg gagagttgcc tgatccgctg
     6241 caggtgccgt ttgatctccg aggcccacac cgc
//
LOCUS       DASPRR010000137         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1000854, whole genome shotgun sequence.
ACCESSION   DASPRR010000137 DASPRR010000000
VERSION     DASPRR010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000854"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..395)
                     /locus_tag="VEL68_05080"
     CDS             complement(150..395)
                     /locus_tag="VEL68_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXZ35373.1"
                     /translation="MKGEEKAEKIEIDRRYCKGCMLCQYVCKFEVFEPGGERSALDYL
                     MPRPARVENCRVCRLCEIHCPDMALTVVAKRKGGGKK"
     gene            complement(392..1228)
                     /locus_tag="VEL68_05085"
     CDS             complement(392..1228)
                     /locus_tag="VEL68_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014121913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ35374.1"
                     /translation="MNPLGEKYLRKDFLPFLFCPGCGDGMIVRAFLEAVDELGVIDDL
                     ALAAGIGCSAWIPVYIHADVLKILHGRAIPAAIGLKATRPSRRVVVFTGDGDGLAIGG
                     NHLLHAARRNIAITVVVVNNQIYAMTGGQVAPSTQIGSRTQTTPYGNLEMPLDACKVA
                     TAAGATMVSRWSTAHPKALTRTFKKALNHKGFALVEVISQCPVQTGRYILGSGASHFN
                     LQWIKDHCVPVDDPSKMGEVEAQGKIPLGDFLRVEKAEFMEEVQRMNQKAKEARSKGG
                     DR"
     gene            complement(1225..2361)
                     /locus_tag="VEL68_05090"
     CDS             complement(1225..2361)
                     /locus_tag="VEL68_05090"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ35375.1"
                     /translation="MKKGRIKVMQGNEACVEAAILAGCRFFAGYPITPATEILEGMAR
                     RLPLVDGFCLQAEDEIAAIVAVVGASWGGAKAMTATSGPGFCLMQEGIGMAASVESPC
                     VIVNVQRGGPASGQSTSTAQGDLYQARYGSNGDYSLITLCPSTSQEMFDHTIRAFNLA
                     ERFRIPVIILSDEVVAHIREKVFIPDPDQLEIVGRKKPTVPMEEIIPFQAEEDGVPPM
                     PAFNTGYKIPVISQAKGPYGTRGTAKEAEVFIRRLTAKVDRHIGEFAEVLTYGDERAE
                     TALVTFGAVCRAARSAVDMARENGRALRSVEVKTLWPFPDDLLRSRLEGAKRIVVAEM
                     NLGMLRNEVQRVIRDPDIEIELFSKIGGLYPQPQEFLRFLTEER"
     gene            complement(2613..>3294)
                     /locus_tag="VEL68_05095"
     CDS             complement(2613..>3294)
                     /locus_tag="VEL68_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ35376.1"
                     /translation="LEVGIGDVLKGFLSSLSRSTISSLIFYLAAATDPILDLIDAGKV
                     QGVSCNSLRFSASALHRFFSRLEHYKPYFILRPVTLTNSAEMLNRFGVLAINSGLEAD
                     LQGQVNSSHVRGSQLIGGIAGSYDFARNSSISVFALPSTTRQGKISNVVPRVSHVDHT
                     EHEVDVLITEHGIADLRALEPYERARRIVEQCADPKFRENLKAYMEKARLNPGRVPIL
                     VRDEFDIR"
BASE COUNT          647 a          990 c          976 g          681 t
ORIGIN      
        1 ggtgcggcat gcccccccgc gggagaccgg cccataggac tgggcctggg ccacctggag
       61 gtcgggaaaa tgttccatca tctgggccaa aatcacgccc atgagaatga ttccctgccc
      121 gccaaatccc gcaaagacga tctccttcct cattttttcc ccccgccttt ccttttggca
      181 accacggtca gggccatatc cgggcagtgg atttcgcaga gccgacaaac ccggcagttt
      241 tccaccctgg ccggccgggg catgagatag tcgagcgcgc ttcgttcccc tcccggttcg
      301 aacacttcga acttgcaaac atactggcag agcatgcagc ccttgcagta cctgcggtcg
      361 atctcaattt tttccgcctt ttcttcacct ttcaacggtc tcctcctttg gaccgggcct
      421 ccttcgcctt ttgattcatg cgctggacct cttccatgaa ttccgccttt tcgactcgca
      481 gaaaatcccc cagggggatc ttcccctgcg cctcgacctc tcccatcttg gaggggtcat
      541 cgactggcac gcagtggtcc ttgatccact ggaggttgaa gtgggatgcg ccggaaccca
      601 ggatgtacct cccggtctgc accgggcact gggagataac ctccaccagg gcgaagcctt
      661 tgtggttcag agccttctta aaggttcggg tgagcgcctt ggggtgggcg gtggaccagc
      721 ggctgaccat ggtggccccg gccgccgtgg caaccttgca ggcatccagc ggcatctcga
      781 ggttaccgta gggagtggtt tgcgtcctgg accctatctg ggtgctgggg gcgacctggc
      841 ccccggtcat ggcgtagatc tgattgttga ccacgacgac ggtgatggcg atgttccggc
      901 gggcggcgtg gagcaagtgg tttccgccga tggccaggcc atccccgtcc ccggtgaaga
      961 cgacgacccg gcgggacggc cgggtggcct tcagcccgat ggccgcgggg atggcgcggc
     1021 cgtggaggat cttgaggaca tcggcgtgga tgtaaacggg gatccaggcg ctgcagccga
     1081 ttccggcggc cagagccagg tcgtcgatga cccccagctc atccacggcc tccaggaaag
     1141 cccggacgat cattccgtcg ccgcagcccg gacaaaagag aaaaggaaga aaatccttcc
     1201 tcaggtattt ttcaccgagt gggttcatcg ctcctccgta aggaaccgca ggaactcctg
     1261 cggctgcggg taaaggccgc cgatcttcga gaacagctca atttcgatat ccgggtcacg
     1321 aatgactctt tgcacctcgt tgcgcagcat gcccaggttc atctccgcca ccacaatgcg
     1381 tttggccccc tccagcctgc tccgaagaag atcatcggga aaaggccaga gggtcttcac
     1441 ttccacggac cggagcgcgc ggccattctc ccgggccata tcgacggcgc tccgtgccgc
     1501 ccggcagacc gcgccgaagg tgacgagggc tgtctccgcc cgctcgtccc cgtaggttaa
     1561 aacctcggcg aattcgccaa tgtgccggtc cactttggcg gtcagccggc ggatgaacac
     1621 ctcggcttcc ttggccgttc cccgggtgcc gtaggggccc ttggcctggg agatcacggg
     1681 aatcttgtac cccgtgttga aggccggcat gggcgggacg ccgtcttcct ctgcctggaa
     1741 gggaatgatc tcttccatgg gaacggtggg cttcttccgg ccgacgatct ccaactggtc
     1801 cgggtccggg ataaaaactt tttcccggat gtgggcgacg acttcgtcgg acagaatgat
     1861 gacagggatc cggaaacgtt cggccaggtt gaaggcccgg atggtgtggt cgaacatctc
     1921 ctggctcgtg gaggggcaca gggtgatgag cgaatagtct ccgttggacc cgtagcgcgc
     1981 ctggtatagg tcgccctggg cagtggaggt ggactggccc gaagccggac cgcccctctg
     2041 cacgttgacg atgacacacg gggattcgac gctggcagcc atgccgattc cttcctgcat
     2101 gaggcagaag ccgggcccgc tcgtggcggt catcgccttc gccccgcccc aggaggcccc
     2161 gacaacggcc acgatggccg cgatttcatc ctccgcctgc aggcagaagc catccaccag
     2221 gggcagacgc cgcgccatcc cttccaggat ctcggtagcc ggcgtgatcg ggtagccggc
     2281 gaaaaagcgg cagcccgcca ggatggccgc ttccacgcag gcctcgtttc cctgcatcac
     2341 cttgatgcga ccttttttca tgtctatgtt tcttcctttg cacttgaaga ttttaccgat
     2401 ggacaaggag aatagattaa ataataaaat tgtttagcca cagaggtcgc agagagcaca
     2461 gagatatagg aacataaaat ggaacagaat ttgatcctgc gtcctcaaaa ccgattgctt
     2521 aggcgtcagc tttttatttt tgcttctcga agttgaatcc ctctgaggcc tcgatggcct
     2581 ctgtgaccaa taatccattc ttggctaaag tgctaccgaa tgtcaaactc atcccttacc
     2641 agaatcggga cccggcccgg attgagcctg gctttttcca tataggcttt taggttctcc
     2701 cggaacttcg ggtccgcaca ctgttctaca atccgccggg ccctttcgta gggttcgaga
     2761 gctcgcagat cggcgattcc atgctccgta atgagaacat cgacctcgtg ttcggtatga
     2821 tccacatggg agacccgggg aacgacgttg gaaatcttcc cctgtcgggt tgtggaggga
     2881 agggcaaaaa cggagatgga gctgttgcgg gcaaagtcat aggaccccgc aattcccccg
     2941 atgagctggg agccccttac gtggctcgag ttgacctggc cctgaaggtc ggcttccagg
     3001 cccgaattga tcgccaggac gccaaaccgg ttaagcattt ccgccgagtt ggtcagcgtc
     3061 acggggcgga ggatgaaata cggtttgtaa tgttccagac gggaaaagaa acggtgcagg
     3121 gccgaggcgg aaaagcgcag gctattacag gatactcctt gcaccttgcc cgcatcaatc
     3181 aggtccaaga tggggtccgt ggccgctgcc aggtaaaaga tgagggagga tatagtactg
     3241 cggctcaaag aagaaaggaa ccccttcagg acgtcgccaa tccccacctc caag
//
LOCUS       DASPRR010000138        14363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_351297, whole genome shotgun sequence.
ACCESSION   DASPRR010000138 DASPRR010000000
VERSION     DASPRR010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14363)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14363
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351297"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VEL68_05100"
     CDS             <1..772
                     /locus_tag="VEL68_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35377.1"
                     /translation="GLTQLHFSSNIWVCLLFAMLAAGLAAFLIGLVIIRKSGIYFGLL
                     TVAFTQMFFTIAFRWRELTGGEDGLKFQPFSWGPLDLKSPLTLYYFVLVLFLVSMVLL
                     YKITKSPVGRVMRANKYNRDRTMVLGYNIYRYRLFAYILSALFSGLAGGLAGVRIAAA
                     FAEPMTWVASGDVVMMVILGGGLANFFGPVLGAAIFSILQDLISTVTIHWMFIFGMLF
                     VFVILVMPEGILGFFSKDQGAYQAFRQRMLKSLGMKTD"
     gene            881..2137
                     /locus_tag="VEL68_05105"
     CDS             881..2137
                     /locus_tag="VEL68_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35378.1"
                     /translation="MTEKKISRRQVLKGALAGAAAASTVHLWPKILRAQEKVLHIGVA
                     SPASGNFSDMGTAEQRGVDLAVRDFNAKGGVLGHKVEVLIEDDQSNPMVAARKARALI
                     QRDKVKFLMGGISSATCIAVGSVAAIEKVIYVATNGNAAEITGEKCNRYTFRVPAEIP
                     SMARFCAEYLIKNVGKKWFLFTHDYAAGHSARDAYKAISAKEGGTIVGDELIPMGTTD
                     FSPFLLKVRQAKPDVLVGNVWGTDGINLLKQGFEFGITKEFPFASTPMDFLDHWAVGP
                     DVSRGYGVVEWYHLGHPGAKAFIEKYQKVYPSSVVLVPENNCYDGYVGTKALLLAIEK
                     AGTTTDVPKVVEALENLKFNEPINDSDVWIRNWDHQFMMNFYLVKSKLPNEMKDRTDY
                     FEILKIAPTADYSVTKAETKCKMGSL"
     gene            2231..2974
                     /locus_tag="VEL68_05110"
     CDS             2231..2974
                     /locus_tag="VEL68_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007142081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35379.1"
                     /translation="MPILSTEKVTKKFGSLVATDRVDLEVTKGSLTAIIGPNGAGKTT
                     FFNLLTGLLEVDGGRIFFDGKEITRLPPFERVKLGISRAFQVVNVFKELSVFENMAIA
                     AQAGTPYGRDLFTDVRSLDDVNKRSEGILERMGLTRKKESLAGHLSHGEMKILDIGLA
                     LASSPKLILLDEPTSGLSPAESQEMTRLIQELATSLTVILIEHDMDIVLNIAQTITVL
                     QQGAVIARGTPEEIRRNERVQQAYLGGVI"
     gene            2974..3678
                     /locus_tag="VEL68_05115"
     CDS             2974..3678
                     /locus_tag="VEL68_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35380.1"
                     /translation="MLLSLEKINTFYGLAHILYDVTLSVGEGETVSLLGRNGAGKTTT
                     LRSIMGLTPPTSGTIRYKGEAINGLPNYVIANKGIGYVPDDRRIFSNLTVEQNLIVSI
                     RKEAPRQNWNIEKVYSHFPQLSPLRKRKGWNLSGGEQQMLAIARALMLNPDLILVDEP
                     TEGLAPIIVKEVAKVLEELSGEKITILLVEQNAKLALNLSSRCYVLSDGHLFYEGVCN
                     VVKKDPDLMKRCFGVF"
     gene            3873..4652
                     /locus_tag="VEL68_05120"
     CDS             3873..4652
                     /locus_tag="VEL68_05120"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ35381.1"
                     /translation="MGVEHVTAIVTGGGQGIGRHIALRLGRRGNCIVIADINEEGSFE
                     TAERILKQGGLAKVIPTDITCEEQVKNLMERALQMNQRIDILVNNSGIAGPIKDIEAI
                     DVEEWERTLAVNLRGMFLCCKHAVPAMKRQKKGAIVNIASITGKRPLPQRTPYAATKM
                     GVIGFTRSLAAELGGWKIRVNAVCPGEVIGPRLDAVLAGIAKFSGENMEQVVAERVEL
                     SPLKTFVDPNYVAAVVDFLCSEDAGMITGQDINVSAGLVMY"
     gene            4674..5678
                     /locus_tag="VEL68_05125"
     CDS             4674..5678
                     /locus_tag="VEL68_05125"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ35382.1"
                     /translation="MKRMAVDRITKADLEREEPMKIGFIGLGTMGHSMASNLIKAGYE
                     LVVHDLRRESARPHCQAGAVWADTPQAVAEAAEVIFTSLPGPSEVEAVALGDRGLVAG
                     LIAGKVYFDLTTNAPSLVRRIHKVFMERGSHMLDAPVSGGPQGAETRKLAIWVGGDEG
                     IFTRYKPVLDAIGDQPYYVGPIGAGSIAKLVHNCAGYVIQTALAEVFTMGVKAGADPL
                     SLWKAVRQGALGRSRTFDRLVDQFLPAKFDPAAFTLRLAHKDVTLATSLGREHKVPMR
                     LANITLEEMTEALNRGWGERDSRVAMLLQEERAGVEIRVLEAALRQVLEAEKKAPGSV
                     "
     gene            5724..7085
                     /locus_tag="VEL68_05130"
     CDS             5724..7085
                     /locus_tag="VEL68_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ35383.1"
                     /translation="MDREIIMDSLSSYISGAGKRDLPGPVSEKGKHHILDTIAAMVSG
                     SRLKTGELAIRFIRMQGGTEEAQVIGSSFLTAAINAAMVNGFLAHADETDDSHAPSFT
                     HPGCAIVPAALAVAERENAPGEEFLRAVVVGYDISSRIARIMSLTPGRLQGHATHSIG
                     PLFGSMAAAASLAKLSPRQVRYALAYTGQQASGITSWPRDKEHIEKAFVFAGMGARNG
                     VTSVLFVQNGFTGEENIFSGPGNFLDIFCPGRDELPKWIDNLSSHYEISITNIKKFCV
                     GSPIQAAAEAMTVLVQKHGLTAAKVKAIDVHLPPQGAQITDNRNMPDINCQYIMAVIL
                     LDGRLAFRAAESYERMSDPQVLEVRSRVNLIGDPRFADQERQRPGLVRVHLTDGTTVE
                     EFVPAVRGTADNPMTRDEVEAKSVDLLQDVLGKKRTQSLVGKIWGLEKVKSVRELRPF
                     LCA"
     gene            complement(7126..10809)
                     /locus_tag="VEL68_05135"
     CDS             complement(7126..10809)
                     /locus_tag="VEL68_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HXZ35384.1"
                     /translation="MKKVLLWVIGILGVISILLVLLLLFLPFLVNLDSVKHKILAHIS
                     KEIGGEAQFHKVSVSLLPRPQVVIQEAKIGIPDKITIAMEGLTIVPQVIPLLIGKLRI
                     AFLEAKGPAVTMELPAQRPKKEGPLDLSSQMIADSLSPLLRLMESKVPQLTLLLEKGQ
                     FTLTKGHQRVFWFRDIQGRAHFPPDQLQVELTCKSNLWEGMSIAAQLSSKDLTGTVNL
                     QVTDMYPRALLNYLVPAAFVIGEGSRGDLTISLEVDRGKFFRGKIRSTSPSLAFQRAE
                     KKWFVKGLTLNGAFQMQGDKAAISLTELFLENPRARLSGELNVDLAAPRYRLNLVGRE
                     MDVYPVREIVMGLVAETEPVRTIFDILREGKIPDISFETHGRTSADLGQLKNISIHGN
                     LVGGRIFLSELLTGLQGIHFDLTQARGNVRLSRGILEGENLAAQWEKARVSKGSLRLG
                     LEGEDAPFHLEALADVDLTPLPPFLKKLIRSQTFAEEMDRFQEWQGKGRVRLTVGESL
                     KSIQPRVDVDELSLLTRYERIPYPLKVERAQGGYDGRKIEVKNLTGAVGRSSFSDVSA
                     QINLGEAPDILVAGGKSFIVLEEIYSWLNSIEKLKAPLQEVRSLKGNVTLSSINLKGP
                     LSQPEKWDYRLGGEIEKLAMDASIMPGPLTLTAAKFEVNPEKILLQDSQVNLLDASLR
                     VSAAVNGWQQRLPIAEATFQGNLGPKVMEWSSARFHLPANLRIRAPLTISRARLGWGP
                     KDGISFTGNWQWPGGPDASVNLLYTPEVLTVNRLLITDGGSQAEIGLKLQAMELRLDF
                     NGELQKTTVDRILVKNEFLAGSIRGDFRSHFFFDAPTRSTAKGRLAGDDLDLALVAKL
                     PLVLKNFALEADKNIIRVQSAALSWEDRHVTVEGSVDFSAGAFLLDMDVAIDGLGWEK
                     VKEVLKPEAQKTEAAQAGKGKFPPLRGRIGFKTPYFTYEKLTWRPLHLEVAFLPEGVN
                     VTIAEAEVCGILTPGSVMATPQGIMLNFHPRVVDQDFSSSMECLLGRPFAMSGTFNFH
                     GQIRGQGQPQDLVRSLQGPLELDLKDGRIYRESIIVRILAFLNLTDLLEKDRRDRTKG
                     EMGYKTIKAKGELQSGKLSIQELHMDASVMQLVSQGEIDWVNQNIDVTAAVAPLKTVD
                     WIVQRIPIVDYILEGTLISIPVRVHGNLNDPKIIPLDPSLIGSDLVGIMKRTLKLPFK
                     LVQPLVKDLKEPSQKPSGDPKRE"
     gene            complement(11264..12160)
                     /locus_tag="VEL68_05140"
     CDS             complement(11264..12160)
                     /locus_tag="VEL68_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ35385.1"
                     /translation="MEVGMEMGTGKDEYRVSGEGRKPRLITPPKACETHSHIFGPADQ
                     YPRFPGRKPPNWTASLEAYQTMLHRLGIERCVIVQPSLYSTDNRCTLDAIRHLGPSRA
                     RGVAVTKKDVGPGELQRLHDAGVRGLRFYLIVDDFKMNDAPEMARRIAPFGWHLQVQD
                     RGNWLPGAIPILEKLPVDIVIDHIGRTPPENGVNDPGFQALLRFMETGRCWVKISAPY
                     LASTDGPPRYADVGDKVRALVSVRPDRLVWAANWPHPNHTPGHRPEEADCLDVLLDWV
                     PDAPIRNAILAENPAALYGFDK"
     gene            complement(12188..13696)
                     /locus_tag="VEL68_05145"
     CDS             complement(12188..13696)
                     /locus_tag="VEL68_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ35386.1"
                     /translation="MDLQFLHNLSLGFVVALQGSNLVYCLIGVLLGTLIGVLPGIGPV
                     ATIAMLLPATFSLSPVGALIMLAGIYYGAQYGGSTTSILVNIPGEAASVVTCLDGYQM
                     ARQGRAGPALATAAIGSFFAGCVSTVVVAMFAPPLAGIALKFGPAEYFSLMVFGLVAA
                     IVLAHGSLLKAIAMVLLGLLLGLVGTDVSSGEMRYTFGIFELTDGIGFVGVVMGLFGI
                     AEIIANLEVKEAREVFTSKVGRLMPSKEDFRRMWSPILRGTAFGSALGILPGGGALLA
                     SFGAYALEKKVSRYGHEFGKGAIEGVAAPESANNAGAQTSFIPLLTLGIPSNVVMALM
                     IGAMMIQGIIPGPRVMTEKPELFWGLVASMWIGNAILVILNLPLIGMWVKLLTVPYRL
                     LYPSILVFMAIGVYSLSSSPFDVLLMAFFGILGHIFTRLECEPAPLILGFVLGPMMEE
                     NLRRALVLSRGDPMVLLTKPISVGFLIAAVILLGIVLAPAIRRKREEVFVED"
     gene            complement(13708..>14363)
                     /locus_tag="VEL68_05150"
     CDS             complement(13708..>14363)
                     /locus_tag="VEL68_05150"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ35387.1"
                     /translation="LRQAHPGRLRKVGPDYQGGGDQGRISPSRLRPTPGGGRGKRGPA
                     AEGVRRPDTGTAGIWFSREMARMVKIKSPRDFWAGLMFIAFGLFFMIGARSYRLGSAA
                     RMGPAYFPTMLGGLLAVIGGIVFLRSLVAKGEKVAALPLRLLFLITLSLLGFGYLLQP
                     IGLVLALALLIILSAWAGHEFRLGEVLLLSVVLVLLSVLVFAKGLGLPFPLWPKFPG"
BASE COUNT         3302 a         3902 c         4102 g         3057 t
ORIGIN      
        1 tgggctgacc caacttcatt tttcgtcaaa catctgggtc tgcctccttt ttgccatgct
       61 ggctgcagga ctggcggcgt ttctgatcgg cctggtgatc atccggaaga gcgggatcta
      121 tttcggcctt ctgacggtcg cattcaccca gatgtttttt accatcgctt ttcggtggag
      181 ggagctgacc ggaggagagg atggtttgaa gtttcaaccc ttctcctggg gccctctgga
      241 cctgaaatcc ccgttgacgc tctattattt cgtacttgtt ctctttctgg tttcgatggt
      301 cctcctttat aagatcacga aatctcccgt agggcgggtg atgagggcaa acaagtataa
      361 tcgtgatcgg accatggtcc tgggttataa tatctaccgg tatcgacttt ttgcctacat
      421 cctttcggcg ctgttttcgg gcctggccgg tgggctcgcg ggcgttcgaa tcgccgccgc
      481 ctttgcggag cctatgacct gggtggcttc gggtgacgtc gtgatgatgg tcattctcgg
      541 cggggggctg gccaacttct tcggccctgt cctgggtgca gccatttttt ccattctgca
      601 ggacctcatc agcacggtta ccatccactg gatgttcatc tttggaatgc tttttgtctt
      661 cgtcattctg gttatgccgg agggcatcct cgggttcttt tcaaaagatc aaggagccta
      721 ccaggctttt cgtcagcgga tgttgaaatc tctaggcatg aagacggatt gaaaccggac
      781 ccacgaaagg gggtggtaat cgcttatttg atgatcaggg ataaagctag tcgggatttt
      841 tttcggattt ttataagaaa atgaaaggag ggtttggatc atgaccgaga agaagatttc
      901 gagacgacag gttttaaaag gggcgttggc cggtgcggca gccgccagta cggtccacct
      961 gtggccgaag atcctccggg cgcaggaaaa agtgctccac atcggggttg ccagtccggc
     1021 aagcggaaac ttctccgata tgggcactgc ggagcagagg ggcgtcgatt tggccgtcag
     1081 ggatttcaat gccaagggag gcgtcttagg gcacaaggtc gaggtcctca ttgaagatga
     1141 ccagtccaat cccatggtcg ctgccagaaa agcccgagcc ctcattcaac gggataaggt
     1201 gaaatttctt atgggcggca tcagcagcgc aacctgtatc gcggtcggat cggtcgctgc
     1261 tattgagaag gtgatctatg tggcaacgaa tggaaatgcg gcagagatca ccggagagaa
     1321 atgtaatcgc tacaccttcc gcgtccccgc tgaaatacct tcgatggcgc gtttttgcgc
     1381 cgaatatctg attaaaaacg tggggaagaa atggttcctc tttacccatg actatgccgc
     1441 cggtcattcg gcacgagatg catataaagc catttctgcc aaagaaggcg ggacgatcgt
     1501 cggagatgaa ttgatcccca tgggaacgac cgacttcagc cctttcctct taaaagtgag
     1561 gcaggcgaag ccggatgtcc tcgtcggcaa tgtatggggg acggatggga tcaatcttct
     1621 gaagcagggt tttgagttcg gcattacgaa ggagttcccg ttcgcatcca ctcccatgga
     1681 cttccttgac cactgggccg tcgggcccga tgtgtccagg ggatatggtg ttgtcgaatg
     1741 gtaccacctg ggtcatcccg gggcaaaggc gtttattgag aagtatcaga aggtctatcc
     1801 ctcctccgtg gtgttagttc cggaaaataa ctgctatgac ggctacgtcg gcacgaaggc
     1861 gcttctgctc gccatcgaaa aggccgggac cacaaccgat gttcccaaag tcgttgaggc
     1921 cttagaaaac ctgaaattca acgagccgat caatgattcg gacgtctgga ttcgaaattg
     1981 ggatcatcaa ttcatgatga atttctacct ggtcaagtcc aagttgccga atgagatgaa
     2041 agaccggacc gattattttg agatcctgaa aattgcgccg actgcggatt acagcgtaac
     2101 caaggcagaa accaagtgta agatgggatc cctttagact gatttcggag ccctgccctc
     2161 agggcagggc tttcttcgca accaggaagc ataagcaaac ccgagttcca gttcttcgaa
     2221 gggggaagga atgccaattc tgagcacgga gaaggtaacc aagaaatttg gctctcttgt
     2281 ggctacggat cgtgtggacc tggaagtgac gaaagggagt ttaactgcga tcatcgggcc
     2341 caatggggct ggcaagacaa cctttttcaa tctgctcacc ggcttattgg aagtggatgg
     2401 aggtcgaatt ttctttgacg ggaaggagat cacccgactt ccccccttcg agagggtgaa
     2461 gctcggtatc tcccgggcat tccaggtcgt caacgtcttt aaggaactct cggtctttga
     2521 aaatatggcg atcgcagcgc aagcggggac gccttacggc cgggacctct ttacggacgt
     2581 ccgctccctt gacgatgtca acaagcggtc cgaggggatc ctggaacgga tgggactgac
     2641 gagaaaaaaa gagagccttg caggccacct ctctcacggc gagatgaaga tactggatat
     2701 cggccttgcc ctggcatcat cgccgaagtt gatccttctc gacgaaccga cgagcgggct
     2761 gtctcctgcc gaatctcaag agatgaccag gctgatccaa gaacttgcta ccagcctgac
     2821 cgtgatcctg atcgaacatg atatggacat cgtcctcaat attgcccaaa ccatcaccgt
     2881 cctccagcag ggggcggtga tcgccagggg aacccctgaa gaaatccgga gaaacgagag
     2941 agtccagcaa gcctatctgg gaggggtgat ctgatgctgc tttcattgga aaaaatcaat
     3001 actttttatg gcctggccca tattctctat gacgttaccc tctcggttgg cgagggtgaa
     3061 acggttagcc ttctgggaag aaacggagcg gggaaaacga cgacgctcag aagcattatg
     3121 gggttaacac ccccgacctc gggaacgatc cgctacaaag gcgaggcgat caacgggttg
     3181 ccgaactatg tcattgccaa taaggggatc ggatatgttc cggatgaccg gagaatcttt
     3241 tccaacttga cggttgaaca gaatctgatc gttagtatta gaaaggaagc acccagacaa
     3301 aattggaaca tcgagaaagt atacagtcac tttccccagt tatctcccct cagaaagagg
     3361 aagggatgga atctgagcgg aggagagcag cagatgctcg ccattgccag agcgttgatg
     3421 ctcaatcccg atctgatcct ggtggatgag ccgaccgagg gattggcgcc gatcatcgtc
     3481 aaagaagtgg ccaaggtcct tgaagagctg tccggggaga agattacgat cctgctggtg
     3541 gaacagaatg ccaaattggc tcttaaccta tcgagtcgct gctacgtgtt gagtgatgga
     3601 cacctcttct atgaaggggt atgcaatgtc gtaaaaaaag acccggacct tatgaaaagg
     3661 tgtttcggag ttttctaagg ctttacccac gacaaccccc ccaggcttaa aaaagactcc
     3721 caagggaaaa ccgaattcat ggattgcgct attgaatccg catccaagaa gagctatcaa
     3781 actgtacgga gggggtggcg ataggcgtag gagtcaatcc aggagattta cccccaggct
     3841 ctctgggctt tatcaaaatg gggtcttttc ctatgggcgt tgaacatgta actgccattg
     3901 ttactggtgg aggacaaggg attgggcggc acattgctct ccgtctgggg aggcgaggga
     3961 attgtatcgt cattgccgat atcaatgaag aggggtcctt tgagacggcc gaacgaattc
     4021 tgaagcaagg gggcctggca aaagttatcc caaccgatat cacctgtgag gaacaggtca
     4081 agaatctaat ggagagagca ttacaaatga atcagcgtat cgatattctt gtgaacaact
     4141 cggggattgc gggtccgatt aaggatatcg aagctattga tgtcgaggaa tgggaaagaa
     4201 ccctggcggt caatctccga ggaatgttcc tctgctgcaa acacgcggtt ccagccatga
     4261 agaggcaaaa aaagggagcc attgtcaata tcgcctccat aaccgggaaa agaccccttc
     4321 cccaacgcac cccctatgcc gctacaaaga tgggggtgat cggttttacg agatccttgg
     4381 cagcggagtt ggggggttgg aagattcggg ttaatgcggt ttgccccggc gaagtgatcg
     4441 ggccgaggtt ggatgcggtc ctcgccggaa tcgccaaatt ctccggagag aacatggaac
     4501 aggtcgtagc ggagagagtc gaactctctc ccctgaaaac ctttgttgat ccaaactatg
     4561 tggcagcggt tgtcgatttc ttgtgctcgg aagatgcggg catgatcacc ggacaagaca
     4621 tcaatgtctc agcgggcctg gtgatgtatt aaaaagagcc ggagcagtgg ggaatgaaaa
     4681 gaatggctgt ggatcgcatc accaaggcag atctcgaaag agaggaaccc atgaagattg
     4741 gattcatcgg actcggtacc atggggcaca gcatggcgtc taatttgatc aaggcgggct
     4801 acgagctcgt ggtccacgat ttgcggcggg agtcggcccg accgcactgc caggcgggcg
     4861 cggtttgggc ggataccccc caagcagtag ccgaggcggc agaggtgatc ttcacctccc
     4921 tgccgggtcc ctcagaagtg gaggccgttg cgctcggaga tcgggggctt gtcgctgggc
     4981 tcatagcagg caaagtatac ttcgacctta cgaccaatgc accctcgctc gtgcgccgaa
     5041 tccataaggt gttcatggag cgcgggagcc acatgctcga cgcgcctgtg agcggcggcc
     5101 ctcaaggggc cgagacgcgg aagctcgcca tctgggtcgg gggagacgag ggaattttca
     5161 cgcgatacaa accagtgctc gacgcgatcg gcgatcaacc ttattacgtc ggcccgattg
     5221 gcgcgggatc aatcgccaaa ctggtccaca actgcgccgg atatgtcatc caaacggcat
     5281 tggccgaagt attcacgatg ggcgtcaagg cgggggccga tccgctctcg ctatggaagg
     5341 ccgtacgcca gggagcgctc gggcggagcc gcaccttcga ccgtcttgtc gatcagttcc
     5401 ttcccgccaa gttcgacccg gcggcgttca ccttgcgact ggcgcacaag gacgtcacct
     5461 tggcaaccag cctgggtcgg gagcataaag ttcccatgcg ccttgccaat atcaccctcg
     5521 aggagatgac cgaggccctc aaccggggct ggggcgagcg cgactcacgg gtcgcgatgc
     5581 tcctgcagga agaacgggcg ggggttgaga ttcgggtgct cgaggccgcg ttgcgtcagg
     5641 tcctggaagc cgagaaaaag gcacccggat cggtgtaaac aggagaaacc ctattttatg
     5701 tgatatcgtc tgccgaggaa aaaatggata gagaaatcat catggactct ctgagcagct
     5761 acatttccgg ggccgggaag agagatctgc cgggcccggt ctcagagaag ggcaagcacc
     5821 acattctcga taccatcgcc gcgatggtat cgggttcaag actcaagacc ggggaactgg
     5881 ccatccggtt catccggatg cagggcggaa cggaggaggc ccaggtcatc ggttcaagct
     5941 tcttgaccgc ggccatcaat gccgccatgg taaatggctt tttggcccat gccgatgaaa
     6001 cggacgattc ccatgctcct tctttcaccc atcccggctg cgcgatcgtt ccggcagccc
     6061 tggccgtggc cgaacgagaa aacgctccag gagaagaatt cttgcgggca gtggtcgtgg
     6121 ggtatgacat ttcctcccgc atcgcccgta tcatgagtct caccccggga cgcctccagg
     6181 gtcacgccac ccattccatt ggtcccctct tcggttccat ggcggcagcg gccagtttag
     6241 ccaaactaag cccccgccag gtacgctatg cgctcgccta tacgggtcaa caagcctcag
     6301 gcatcacttc ttggccgcgg gacaaggaac acatagaaaa ggcctttgtc tttgccggaa
     6361 tgggggctcg aaatggcgtc acatcagtat tattcgtcca gaatggcttt accggcgagg
     6421 agaatatctt ttctggccct ggcaacttcc tcgacatctt ttgccccgga cgggatgagt
     6481 tgcccaaatg gattgacaat ctgagcagtc attacgagat ttccatcacc aacatcaaaa
     6541 aattctgcgt cggctctccc atccaggctg cggctgaagc catgaccgtt ttggtccaga
     6601 aacatgggct tacggccgca aaggtcaagg ccatcgatgt tcatttaccc ccccagggag
     6661 cacagataac cgataaccga aacatgccgg atatcaactg ccagtacatc atggctgtaa
     6721 tcctcctgga cggccggttg gcttttagag cggccgaatc ctacgaacgt atgtccgacc
     6781 cccaagttct ggaagtccgg tcccgggtga atctcatcgg cgacccccga tttgcggacc
     6841 aggagcgcca gcgtccaggg ctggtcaggg tccatctaac cgatgggacc acggtagaag
     6901 aatttgttcc cgcggtccgg ggcacagcgg ataatccgat gacccgggac gaagtcgagg
     6961 cgaaatcggt tgacctgctg caggatgttt tgggtaagaa acgtacccag tcgctggtcg
     7021 gcaagatttg ggggctggaa aaggtgaaat cggtccggga gctccggccc tttttgtgcg
     7081 cctgatttct aaaggttcga gaagaattgg tgccaattga aaaaatcatt ctctcttcgg
     7141 atcaccggat ggtttctggc tgggttcttt gaggtctttg accagcggct ggacgagttt
     7201 gaaaggaagc ttgagggtcc gcttcataat cccgacgaga tcggagccga tcaacgaggg
     7261 atcgagagga atgatcttgg ggtcattcaa gttcccatgg acccgcaccg ggattgagat
     7321 aagggttcct tcgaggatgt aatcaacgat gggaatccgc tggacaatcc agtccaccgt
     7381 tttcagcggg gccacggcag ctgtcacgtc aatattttga tttacccaat cgatttcccc
     7441 ctgagaaacc agctgcatga ccgaggcatc catgtgcagc tcttggatcg atagtttccc
     7501 cgattgcaat tctcctttgg ccttgattgt tttataaccc atttctcctt tggtccgatc
     7561 cctccggtcc ttctccagca ggtccgtcaa gttgaggaag gcaagaattc ttacgatgat
     7621 tgattcacga tagatgcgcc cgtccttcag gtccagttcc aagggcccct ggagagaccg
     7681 taccagatct tggggttgcc cttgacccct gatttggcca tgaaagttaa aggtcccgga
     7741 catggcaaac ggcctgccca ataggcattc catgctcgag gaaaagtcct ggtctacgac
     7801 gcgggggtga aaattcaaca ttatcccctg aggagtggcc atcacggatc ccggggtaag
     7861 aatcccgcaa acctcggcct cggcaatcgt tacgttcacg ccttcgggga ggaaagcgac
     7921 ctccagatgc aaaggcctcc aggttaactt ctcgtaggta aagtacgggg tcttgaaccc
     7981 gattctcccc ctcaaaggag ggaacttccc tttcccggct tgggccgcct ctgttttctg
     8041 ggcttcgggt ttcaggactt ctttgacctt ttcccagcca agcccgtcga tggcaacatc
     8101 catatcgagg agaaatgctc ctgcggaaaa atctaccgag ccttccacgg ttacatggcg
     8161 gtcttcccag gaaagggcgg cggattggac acgaatgata ttcttgtcag cctccagggc
     8221 aaaatttttc agaaccagcg gaagcttggc taccagagct agatcaaggt cgtcccctgc
     8281 aagtcttccc ttggctgttg aacgcgtggg ggcatcgaag aaaaaatggg atcggaaatc
     8341 gccgcgaatg gaacccgcaa ggaactcatt ctttaccagg attcgatcaa ctgttgtttt
     8401 ctggagttcc ccattaaaat cgagccgcag ttccatggcc tgcaatttca acccgatttc
     8461 cgcctgagaa ccgccatcgg tgatgagcaa gcgattgacc gtgaggactt caggggtata
     8521 aagcagattg acggatgcgt cgggtccccc gggccattgc caatttccgg tgaatgaaat
     8581 cccatccttc ggcccccacc ccaggcgcgc ccgagaaatc gtaagaggtg cccggattcg
     8641 tagatttgcc ggaagatgga atcgggcgga agaccactcc atgactttgg gtcccaggtt
     8701 cccctggaaa gtcgcctctg caatgggcag cctctgttgc caaccattca cagctgccga
     8761 gactctcaga gaggcgtcta agagattcac ctgagaatcc tggagaagaa tcttctcggg
     8821 attgacctcg aattttgcgg cagtaagggt aaggggacct ggcatgatgg aggcatccat
     8881 cgccagcttc tcgatctccc caccaaggcg atagtcccat ttttccggtt ggctcagggg
     8941 gcctttcagg tttatggagg acagggtcac gttgcctttc aaagaccgga cctcctgtaa
     9001 tggagccttg agcttttcga tcgagttgag ccaggaatag atttcctcca gcacgatgaa
     9061 ggactttccc cctgccacaa gaatatcagg agcttcccca aggttgattt gggcggaaac
     9121 atccgaaaaa gaagaccgcc cgactgctcc cgtaaggttc ttgacctcaa ttttcctccc
     9181 atcatacccg ccctgggccc tttcaacttt caaaggataa ggaattcttt cgtaacgggt
     9241 gagtaagctc aattcatcga catcaaccct cggttggatc gatttaaggg actctcccac
     9301 agtcaacctt acccggcctt tcccctgcca ttcctggaag cggtccatct cttcggcaaa
     9361 agtctgactc cggatgagtt ttttcaggaa gggaggaagc ggagtgaggt cgacatcggc
     9421 cagggcttcc agatggaaag gagcatcctc gccttccagc cccagcctca atgaaccctt
     9481 ggaaactcgg gccttctccc attgggcggc aaggttttcc ccctccagga ttccccgcga
     9541 aaggcggacg tttccccggg cttgagtaag gtcaaaatga atcccttgga ggccggtcag
     9601 caattcggaa agaaaaattc ttccgccaac caggttcccg tgaatcgaga tgttcttcag
     9661 ctggccaagg tctgccgagg tacggccatg cgtttcgaag gaaatgtcgg ggattttccc
     9721 ttcccggaga atgtcaaaga tggttcggac cggctcggtc tccgcaacca agcccatgac
     9781 gatttccctc acgggatata catccatctc ccggccgacg aggttcaggc ggtagcgggg
     9841 ggccgccaga tcgacattca attctcccga taatctcgcc cggggatttt ccaggaaaag
     9901 ctccgtaagg gagatggctg ccttgtctcc ctgcatctga aaggccccgt taagggttag
     9961 ccccttcacg aaccatttct tctctgctcg ctgaaacgcc agagagggtg aggtactccg
    10021 gatttttccc cggaagaact tgccccggtc tacctccagg ctgatcgtca aatctccccg
    10081 cgatccttcc ccaatgacga aggcagcagg aacgaggtaa ttcagaagcg cccgggggta
    10141 catgtccgtg acttgcagat ttacggtccc ggttaaatcc ttcgaactca actgggctgc
    10201 gatggacatt ccctcccaaa gattggattt gcaggtaagc tccacctgga gttgatcagg
    10261 agggaaatgg gcacggccct gaatatctcg aaaccagaag accctctgat ggcctttggt
    10321 gagggtgaat tgtccttttt ccaaaagaag ggtgagctgg gggactttgg attccatgag
    10381 cctcaagaga gggctcaagg aatcggcgat catctgggaa gataagtcta gaggtccctc
    10441 cttttttggc ctttgagcgg gaagctccat ggtaacagcg ggaccttttg cttcaaggaa
    10501 ggcgatcctt aattttccaa tgagcagcgg gatgacctgg gggacaatgg taagcccttc
    10561 catagctatc gttattttat cggggatccc gatcttagcc tcttgaatca caacttgggg
    10621 acggggaagg agagagacgg acaccttatg aaactgggcc tctccgccga tttccttgga
    10681 aatatgagcg aggatcttgt gtttaacgga atccaaattg acaaggaagg ggagaaagag
    10741 aagcaagagg acgagcagga tggaaatgac ccccagaatt ccaatgaccc agagaagtac
    10801 tttcttcatt ttgctcatgg agaaactaga ccttttctgg aatttccatc attctcttgg
    10861 atcccctttg gccagatttg aaccgttccc atgatctttc aaaaaatcct ttgcgcaggg
    10921 gtggcaggat tttgcccggc caggagccaa cttgaataac ttgtaatctt cgttttttgc
    10981 cgaataaatt caatgttaca acgtcacccc gcttctgaca aggcgaacaa gggggccgcc
    11041 ctgaagagga actggggccg gcagaggggt ccaacattaa cgggaaaaac gacctgacgc
    11101 agcccattga agacgccgta aagggctcgc ccatgagata aattctgcct caacccccat
    11161 ccttaaaagg cgggaaccag cagaattaaa tcgggagggt gtgaaaattt ctttgcagtc
    11221 tcttccgaaa gaacaaggtg gggactaaac cgccccccct cggtcatttg tcaaaaccat
    11281 agagcgccgc cggattttca gccaggatgg cattccggat gggcgcatcg ggcacccagt
    11341 cgagcagaac atcaagacag tccgcctctt caggccgatg gcccggcgtg tgattgggat
    11401 ggggccagtt ggccgcccaa accaggcgat cgggccgaac ggataccagg gcccggacct
    11461 tgtcccccac atcggcataa cgcggcggtc cgtcggtgct ggcgagatag ggggcggaaa
    11521 tttttaccca gcaccggccc gtttccatga agcgaagcag cgcctggaag cccggatcgt
    11581 tgaccccgtt ttccggcggg gtccgaccga tgtggtcgat gacgatatcc accggcagct
    11641 tctcgaggat cggaatcgcc cccggcagcc agttgccgcg gtcctgaacc tggagatgcc
    11701 agccgaacgg cgcgatgcgc ctggccattt caggggcgtc attcatttta aagtcgtcaa
    11761 cgataaggta gaatcgcaat cctctcaccc cggcatcgtg gagccgctgc agttccccgg
    11821 gaccgacgtc cttcttggtc accgcgacgc ctcgcgcccg ggatggcccc agatgccgga
    11881 tggcgtcgag cgtgcaccgg ttgtccgtgc tgtatagtga aggctgaaca atgacacatc
    11941 gctctatgcc cagtcgatgg agcatggttt ggtaagcctc gagggatgcg gtccagttcg
    12001 gtggtttccg accgggaaag cgggggtatt ggtctgccgg tccgaagatg tgcgagtgcg
    12061 tttcgcaggc cttcggcggg gttatcaatc gcggtttgcg accctcgccc gagacccggt
    12121 attcatcctt ccctgtgccc atttccattc cgacctccat ttctgattcg atcgatagcc
    12181 catctggcta gtcttccacg aagacctctt cgcgcttcct gcgaatggcc ggcgccagga
    12241 cgatgcccag caggatgacc gcggcgatga gaaagccgac gctgatcggc ttggtgagca
    12301 agaccatcgg gtcgccgcgc gacaggacca gcgcgcggcg gaggttctcc tccatcatcg
    12361 gccccaggac gaaacccaag atcaacggcg ccggctcgca ttcgagtctg gtgaagatgt
    12421 ggccgagaat cccgaagaac gccatcagga gcacgtcgaa agggctgctc gacaggctat
    12481 acactccgat ggccatgaac accaggatcg aggggtagag caatcgatag ggcacggtga
    12541 gcagcttgac ccacatgccg atcagcggca ggttgagaat cacgagtatg gcgttgccga
    12601 tccacatgga agcaaccaga ccccagaaaa gttctggctt ctcggtcatc accctggggc
    12661 ccgggatgat tccctgaatc atcatcgccc cgatcatcaa ggccattacc acgttggagg
    12721 ggatccccaa cgtaagcagc ggaataaagg aggtctgcgc gccggcgttg ttggccgact
    12781 ccggcgccgc gacgccctcg atggccccct tgccgaactc gtggccgtaa cgcgatactt
    12841 tcttctccag cgcgtaggcg ccgaacgaag ccagcaaggc cccgccgccg gggaggatgc
    12901 cgagggctga accgaatgcc gtcccgcgca ggatggggct ccacatgcgc cggaaatcct
    12961 ccttcgacgg catcaggcgt ccgaccttgc tggtgaagac ctcacgtgcc tccttgactt
    13021 ccaggttggc gataatttcg gctatgccga acagccccat gaccacgccg acgaacccga
    13081 tcccgtcggt gagttcaaaa ataccgaagg tgtagcgcat ctcccctgag ctgacgtcgg
    13141 tgccaaccag gccaagcagg agacccagca acaccatggc gatcgccttg agcaaagacc
    13201 cgtgcgccag gacgatggcg gccaccaggc cgaacaccat cagcgaaaaa tactcggctg
    13261 gcccgaactt cagcgcgatt ccggccaagg gtggggcgaa catggccacg actacggtgg
    13321 aaacacagcc ggcgaagaac gacccgatcg ccgccgtggc cagcgccgga cccgccctcc
    13381 cctggcgcgc catctggtag ccgtccagac aggtcaccac cgatgctgcc tcgccgggga
    13441 tgttgaccag gatcgatgtg gtcgagccgc catactgcgc gccgtaatag atgccagcca
    13501 gcatgatcaa cgccccgacc ggcgagaggc tgaaggtggc cggcaggagc atggcgatgg
    13561 ttgccaccgg gccgatgccc ggcagcacgc cgatcagtgt tccgagcaga acaccgatca
    13621 ggcagtagac caaattcgac ccttgcagcg caaccacgaa gccgagcgaa agattgtgca
    13681 ggaattgcag gtccatggcc ttctccttca gcctgggaat ttaggccaga gagggaaggg
    13741 aagcccgaga cctttggcga aaaccagcac ggacaggagg accagcacga cggaaagaag
    13801 gagcacttcc ccaagcctga attcgtgccc ggcccacgcg gagaggatga tcagaagcgc
    13861 cagggccagc accagaccga tcggctggag caggtagccg aaccccagca gcgacagggt
    13921 gattaagaaa agcagcctca ggggaagcgc agcaaccttt tctcccttgg ccacgagtga
    13981 ccggagaaac acgatacccc cgatcaccgc caagagcccc ccaagcatcg tcgggaaata
    14041 ggccggcccc atgcgtgccg cgctgcccag ccggtagctg cgggcgccga tcatgaagaa
    14101 tagaccgaag gcgatgaaca tcaatccggc ccagaaatct cttgggcttt tgatcttgac
    14161 cattcttgcc atttcccggg aaaaccagat ccctgcggtc cccgtgtccg ggcggcgcac
    14221 gccttcggcg gcaggacccc gtttgcccct gccccctcct ggggtgggcc gcaggcggga
    14281 ggggcttatt cgcccttgat ccccgccgcc ttgataatcg ggccccactt ttcgaagtcg
    14341 gcccggatga gcttggcgaa gtc
//
LOCUS       DASPRR010000139        25094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1159834, whole genome shotgun sequence.
ACCESSION   DASPRR010000139 DASPRR010000000
VERSION     DASPRR010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 25094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25094)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25094
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159834"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..933)
                     /gene="prfB"
                     /locus_tag="VEL68_05155"
                     /pseudo
     CDS             complement(1..933)
                     /gene="prfB"
                     /locus_tag="VEL68_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157471875.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(944..2491)
                     /gene="lnt"
                     /locus_tag="VEL68_05160"
     CDS             complement(944..2491)
                     /gene="lnt"
                     /locus_tag="VEL68_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXZ35388.1"
                     /translation="MRLPALFLAALSGLLLILAFPKFNAEIVAWAALIPFFWSIRRAS
                     PSQAALLGFVCGFSFFTGLLPWIYNVLTQYGHLPGAVSVFFLLLLTAYLALYFSAFAF
                     ALRWVEARVEIPETILAPPLWVSLEYIRGFLFSGFPWELLGYSQYLTLPMVQIADITG
                     VYGVSFLLVLINAAGYRLLEAFADRKWRLVRGEILAAGFLLVLTAGYGYWRLAGMSPE
                     SKEGTPVRIALVQGNIPQDVKWEPKFQEETMRIYTDLTLRTQATLPQLVIWPETATPF
                     FFQDSFPYQSRILDLAQKMKTHLLFGSPAYDRKGRETRYYNSAFLISPEGKVTGRYDK
                     IHLVPFGEYAPLSGILGFTRDIIGAMGDFQSGEEVRDLDFSQGKLGVLICYEAILPDL
                     TRQFVDRGAQFLVNITNDAWFGHTAAPYQHISMAALRAVENRVPMARAANTGVSGFIH
                     PSGRILQASDIFTKAILFDNIYIKNRWSFYTQFGDLFTYLCLGYIILFLIYIRFRRED
                     RVERNRE"
     gene            complement(2504..3499)
                     /gene="moaA"
                     /locus_tag="VEL68_05165"
     CDS             complement(2504..3499)
                     /gene="moaA"
                     /locus_tag="VEL68_05165"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXZ35389.1"
                     /translation="MNGLVDSHNRRLNYLRISITDRCNLRCRYCMPKEGVSQFGHSEI
                     LSYEEILRLAGLAVKRGIDKVRITGGEPLVRKGVVNLVEKLSGLPGVRDLSMTTNAIL
                     LREFARDLRRAGLRRVNISMDSLDPGKYREITRGGDLNQVWKGIETARREGMNPIKIN
                     VVAIAGFNDGEIEDFAHLTRKEPFQVRFIEFMPIGAGSEWKPEHCISCREIKDRIERT
                     SPLVPLAESGNGHGGPARLFKLPGAKGEIGFISPVSEHFCGSCNRLRLTADGKLKTCL
                     FSEALTDLKTLLRSGATDQELERKLDESLLTKPPHHGPMGGVMKRCHLPMVKIGG"
     gene            4165..4848
                     /gene="cmk"
                     /locus_tag="VEL68_05170"
     CDS             4165..4848
                     /gene="cmk"
                     /locus_tag="VEL68_05170"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXZ35390.1"
                     /translation="MEREKGLAIITIDGPAGAGKSTVSRMLARRLNYLYLDTGAMYRA
                     VALQVLKERIDPGDEKALEGLCKGIAIAFAEDSRGQRVFCQGEDVTEKIREPEVSWMA
                     SAVSMKRPVREAMVTLQRRMGAQGGLVAEGRDTGTVVFPRAQAKFFLVADPRERALRR
                     FRELKAKGKDAKPEEVEEDVRKRDVQDSSRDLAPLRPAEDAHLIDSTGLTPEEVVDRM
                     LEVLGRSFS"
     gene            4982..6772
                     /locus_tag="VEL68_05175"
     CDS             4982..6772
                     /locus_tag="VEL68_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXZ35391.1"
                     /translation="MLDKEEQFLTAKPEDRPPTEAAEESYENKGKPEEGESFQELYEE
                     SLHEIAEGEVVHGRVIHVGPEFVTVDIGFKSEGQLSLNEFRQKDGTLSVNIGDEIDVF
                     VERKESEIGMVTLSKEKADKFKFWEEISRAWNEDQVIEGKIISRIKGGLTVDIGVLAF
                     LPGSQVDIRPIRNLEKLIGSTHKFKIIKLNRRRGNVVLSRRILLEKERESIRQKTLEV
                     LQEGQIVEGVVKNITDYGAFIDLGGIDGLMHITDMSWGRINHPADILTVGQKIKVKVL
                     QFERQNQRVSLGLKQTTPDPWDNIAAKFPVGGRVKGKVTSITDYGAFVQLLEGVEGLV
                     HVSEMAWTKRVRHPSKILSAGDEVEVMVLDVDSNQKRISLGLKQTSPNPWDTIAERYP
                     VGTKIQGRIKNITDFGIFIGIDEGIDGLVHISDISWTQRLKHPSEMFKKGQEVQAIVL
                     SIDRENQRFSLGIKQIQKNPWDDIHHRYRVGQMAKGKVTNVTKFGAFVELEPGIEGLV
                     HISEISHQRVEKAEDALKAGSEIQAVVINVDPRRHKIGLSIKDVERYQTQQARKETAA
                     AYEDDTSDFGRMLKEGLERRAREANSSRES"
     gene            6780..7673
                     /gene="sppA"
                     /locus_tag="VEL68_05180"
     CDS             6780..7673
                     /gene="sppA"
                     /locus_tag="VEL68_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXZ35392.1"
                     /translation="MKKHPILLGLTVLGVLLGLFLVSIWVLSYFSNPEESFWGGEKIA
                     VIEVRGVILDPQPIVEKLVKLRKNEKIKAIVLRIDSPGGGVGPAQEIHAEVKKAQKEK
                     KVVVSMGSVAASGGYYIACAADRIIANPGSITGSIGVIVESLNVEELLGKLGLRSVVV
                     KSGKHKDLGSPMRPMTAEDRRLLQGVIDSVHEQFIRAVAEGRKLPLEKVRKLADGRIF
                     SGDQARTLGLVDELGNLEDTIALAAQMAGIRGEPEIIYPEKKRFSLFDLLIQETVQKI
                     VESLKESTPPLNFLYYWPFSG"
     gene            7684..8634
                     /locus_tag="VEL68_05185"
     CDS             7684..8634
                     /locus_tag="VEL68_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HXZ35393.1"
                     /translation="MPLARLASIDVGSNTTRLLLAQPFPDQTFQPLRVERIITRLGGN
                     FFPGKKLNKISIERTLKALRSFAELLEGMGVKKVIAAGTGVLRQAKNRSAFVRAVREQ
                     TGLSLRILSGQEEARATLRGVLGSLRDRNTPRLITDVGGGSTEIIWMEGRTLRETVSL
                     NLGAVGLTEKFLSKDPPSSTEMEALKRFVHSALQRVLRQWERGGLHTQALHPCLVGTA
                     GTATTLGAIDLSLSVYDPRKVNGHRISISKLRRMYRRLSTLPLKERVKLPGLEKGRED
                     LILAGSSILLNLMEVFRRSALEVIDSGLLEGILLEGIGEM"
     gene            8734..10221
                     /gene="guaB"
                     /locus_tag="VEL68_05190"
     CDS             8734..10221
                     /gene="guaB"
                     /locus_tag="VEL68_05190"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXZ35394.1"
                     /translation="MIPENLTESLTFDDVLLLPSESSILPRDVDVTTQLTRAIRLNIP
                     FLSAAMDTVTEAQMAIAMAREGGIGIIHRNMSVTAQAAEVDKVKKSESGMIVDPITMD
                     PDQKIYEALEIMKKYRISGVPVTRKGKLVGILTNRDLRFETQLDQPISAVMTKERLVT
                     AHPGITLEESKRLLHKNRIEKLLVVDEQNNLKGLITIKDIEKTIKYPNSCKDQLGRLR
                     VGAAVGPGTDRDARVEALLKAGADVIVIDTAHGHSLNVLESIRAVKKLFPDCQLVAGN
                     VATAEGTAALIQAGVDAVKIGVGPGSICTTRVIAGVGVPQITAIAESARVAQKYHIPI
                     ISDGGIKFSGDVTKALAAGADTVMIGSLFAGTDESPGEMVLYQGRSYKMYRGMGSIEA
                     MKGGSKDRYGQDDVESEMKLVPEGIEGRVPYKGPISSSIYQLLGGLRAGMGYVGCRTL
                     KALKEKSRFTKITSAGLRESHVHDVIITKEAPNYSPGELSSWKES"
     gene            10227..11783
                     /gene="guaA"
                     /locus_tag="VEL68_05195"
     CDS             10227..11783
                     /gene="guaA"
                     /locus_tag="VEL68_05195"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXZ35395.1"
                     /translation="MQVKDFEGRILILDYGSQYTQLIARRVREAHVYCEIHPFHMSLE
                     AVRDFQPKGIILSGGPSSVYEKDSPWNPPDLFRLSIPTLGICYGMQLMTHVLGGRVAK
                     SSKREYGRSELFIDDERDLFAGLESGKAIQAWMSHGDRIEVPPEGFQVIAHSPNSPVA
                     AIRHQDLCAYGVQFHPEVVHTPLGKQILQNFLFRICHCPPDWTMESFVIKTVQFLKEQ
                     IGKEKVICGLSGGVDSSVVAVLLHKAVGSQLTSIFVDNGLLRKDEARKVKGLFGFNLG
                     LNLRVVEAGDRFLDRLAGVMDPEQKRKIIGNEFIYLFEKEAQKAGGATYLAQGTLYPD
                     VIESVSFKGPSAKIKSHHNVGGLPERMHLKLIEPLKELFKDEVRAVGRELGLPPELIE
                     RHPFPGPGLAVRTLGAVNQQDLVLLREADAIVEEEMHRTGWYTRVWQAFAVLLPVCTV
                     GVMGDERTYERVAVLRVVQSLDGMTADWVPLPAEVLGTISNRIINEVRGINRVVYDIS
                     SKPPSTIEWE"
     gene            11906..15370
                     /locus_tag="VEL68_05200"
     CDS             11906..15370
                     /locus_tag="VEL68_05200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXZ35396.1"
                     /translation="MKHADFVHLHLHTQYSLLDGAIRLDDLFEKAKSYRMPALAMTDH
                     GAMFGAIEFYQQAQKTGLKPIIGCEVYVAPTSRFDKNPKGPSEASHHLILLAKNLAGY
                     RNLMKLVSLAHFEGFYYKPRVDKELLQANNEGLIGLSACLQGEVPYYLTGGDKSRALR
                     AADELKSIFPDHRFYLEIQENKIPEQMKVNQGLMEISRELSLPLVATNDCHYLRREDA
                     QAHEVLLCIQTGKTLKDADRMKFSTNEFFFRSAEEMEALFSYCPEAVRNSVEVAERCN
                     LDLKLSELQFPQFQVPPGETLDSFLEKTAQKGLEDRLIEWKALGQSLPVNMEFYRQRL
                     QKEVGMIQKMGFSGYFLIVSDFIRYAKSKGIPVGPGRGSGAGSLAAYCLGITEIDPLV
                     NGLLFERFLNPERVSPPDIDIDFCIQGREEVIQYVRSKYGQDHVAQIITFGKMQARAV
                     IRDVGRALDMPYGEVDRIAKMIPNTLNITLQEALQQEPRLNELGRSNPEVKKLLTLAQ
                     ALEGLPRHASTHAAGVVISNRPLVEVVPLYRGQQNEIMTQYPMKDVEKIGLIKFDFLG
                     LKTLTMIEDVLKRVEASRGERLDLRRISLTDRKTYELLSSGDTSGVFQLESSGMKDLV
                     MRLLPERFEDLVALLALYRPGPLKSGMVDDFIKRKQGKTLITYEIPELKEILEETYGV
                     IVYQEQVMKIATKLASFTLAQADILRRAMGKKNVEEMAMQKKNFIEGTQRGKIAGKKA
                     EKLFDLMAKFAEYGFNKSHSAAYALVAFQTAFLKAHYPVEFMAALLSSEMGNSDKILR
                     HLGECREKKIEVLPPDVNESQQDFTVVSGKIRFGLAAVKNVGLAAIQSILSAREEKGN
                     FTSLADFCLKVDLRKVNKRVIESLIKSGSFESLGFSRAQLLAGLEEAMEWAQGVERER
                     ANHQIAMFGGIRGGKRRDEPRLPELAPWPESQRLAAEKETLGFYLTGHPLSSYAPTLQ
                     RFTSMDTLRVQEAADGQEVILGGVVNSLKEINTKKGDRMAFVSLEDLNGMVEVIIFSE
                     LYKNSSLLLKSEDPLFIKGRVDAGEESVKIIASEVLPFEQAVGKLTTSVHLRVHSEGL
                     RRQDLEAMREIFQACRGSSPAYLHLILPGEKEAVIDLGEDWKLNSTDQLVRRIRDLLG
                     YEAVSFQA"
     gene            15435..16394
                     /locus_tag="VEL68_05205"
     CDS             15435..16394
                     /locus_tag="VEL68_05205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXZ35397.1"
                     /translation="MALYYLDFEKPIAEIDKKIAELKTFSYHPHPRMAEEVASLEKQL
                     QRTLTKVYSNLSRWQRTQLSRHFNRPTFLEYVEQVFSEFLELHGDRKYGDDPAIVGGV
                     AKFQGEPVIVVGHQKGRTVKEKMSRNFGMPNPEGYRKALRLMKMAEQFGKPLITMIDT
                     PGAYPGQGAEERGQALAIAEDLQAMATLRTPIISVIIGEGGSGGALALGVGDRLLMLE
                     NATYSVISPEGCAAILWKGETEKEKAAEALRGTAADLLELGVIDEVLREPLGGAHRNG
                     KEMARTFSESLARHLKGLRGIPMEDLLQQRYEKYRRMGRVLGG"
     gene            16405..17163
                     /locus_tag="VEL68_05210"
     CDS             16405..17163
                     /locus_tag="VEL68_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HXZ35398.1"
                     /translation="MEEKPGGFREAYQERKYRNLLPDQGMVSFQVMVKETDLYIKASR
                     NLAEPARESVLRYRHQLESYIHLHPSFLHSLTPWPTDDFAAPIIQSMIRAGQGAGVGP
                     MAAVAGAMAESVGRDLLKEAPEIIVENWGDIFLRSSRGIPVGIFAGNSPLSLRVGLLV
                     PASEDPSGVCTSSGTVGPSLSFGRADAVCILSPSASLADAAATAVGNRVRSSTDLERG
                     LEQAKEIVGVVGAVIILGDKLAAWGEIELIDLKR"
     gene            17260..17739
                     /locus_tag="VEL68_05215"
     CDS             17260..17739
                     /locus_tag="VEL68_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXZ35399.1"
                     /translation="MIRIGVISDTHLGQPTEELKALLQGSFKEVETILHAGDLTELAV
                     LEAFAGKNLLAVCGNMDAQSVRKQLPTHRVFQAGKFTIGLAHGWGGPQGIEERIAKEF
                     GKVDCIVYGHTHTPSQREREGIFFFNPGAFHGKRGWSSRSVGILNLGGGISGQIIHL"
     gene            17758..18198
                     /locus_tag="VEL68_05220"
     CDS             17758..18198
                     /locus_tag="VEL68_05220"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXZ35400.1"
                     /translation="MIERLFAPWRMNYILADKKGKKCIFCPMDGHSDRERLILQRASH
                     SLVMLNRYPYSYAHLMVAPMRHVDQVQNLNKKEMADILFSLGQSIEILREAFHPDGFN
                     VGMNLGQVAGAGVLHHLHFHIVPRWNGDTNFMRLSERSGSSLST"
     gene            18260..18550
                     /locus_tag="VEL68_05225"
     CDS             18260..18550
                     /locus_tag="VEL68_05225"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HXZ35401.1"
                     /translation="MGLARAIIFFSLLVLGIGFAILNDGPVSLKYYFGLETHPLPLFL
                     WALLFLLLGLILSGLWSFFSKIGLHSRIRHSQRAIADLERERNRLAEERKSL"
     gene            complement(18808..19452)
                     /locus_tag="VEL68_05230"
     CDS             complement(18808..19452)
                     /locus_tag="VEL68_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HXZ35402.1"
                     /translation="MLVRGKMKKDLVTITKDDRMTLAKKLLKEKNIRHLPVVDGKRLI
                     GLVTNMDIRKAEASPATSLEIRELHYLLDKLTVGEIMTRNVITISPDISIEEAAILLH
                     DNKIGCLPVVEDGNLVGILTENDVMEILIEVMGMKEKGSRLEVMVDDKPGALADITRI
                     IKEHNVNITSVVTDRADETGKRLVVFKLKTFYFEPIKKALEAGGFPVLYAKIEK"
     gene            19703..22309
                     /gene="mutS"
                     /locus_tag="VEL68_05235"
     CDS             19703..22309
                     /gene="mutS"
                     /locus_tag="VEL68_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXZ35403.1"
                     /translation="MNNVTPMIQQYLEAKSKCPDAILFYRMGDFYEMFFEDAKIASEI
                     LDIALTSRDKSKEDSVPLCGVPYHSATGYIQRLVDRGFKVAVCEQMEDASQAKGLVRR
                     EIIRIITPGMVLEDELLQPKANNFLMAISLGGEHFGLAILDASTADFLAAEVNTVQSL
                     RDEVLRIAPKEVLLAESAKGQAPVKSLMKAYPQALYSSLPSEVFDEERSRERLKSLGL
                     SLPEGKEQSLRAVGAILFYVQETQKTSPVHLSRLEFYQVQDYLVLDETTRRNLELFEN
                     LQSRSRKGSLIDILDETVTSMGGRMLKRWLGSPLMNMEKIQDRLEKVAELKEKDLPRK
                     ALRETLRQIKDMERLSARISLGVANARDLVALRASLNILPDAKTLLSPMEYKGFKPYL
                     KEMDDLPSIRDLLDRAIEDSPPLSLKEGGLIKRGFDATLDRYRDMGREGKGWIADLEA
                     KERMRTGISSLKVRFNRVFGYYIEVTKANLSSVPSHYMRKQTLANAERFITPELQEYE
                     TQVLHAEEQSEALEYQLFQEVLQKVTAEVPQIQNSAQALAEVDALAALAEVAERYAYN
                     RPQLDEGDTLVIVEGRHPVLERLSQDERFVPNDLFLNGEDRQILVITGPNMAGKSTIM
                     RQAALTVILAQMGSFVPAKEASVCLVDRIFTRVGATDDLVRGRSTFMVEMEEVAHILR
                     NITPRSLILLDEIGRGTSTFDGLSIAWAVTEYLHDSCPFQPKTLFATHYHELTELAMT
                     KPRVKNFHVAVKEWNDRVIFLRKLVEGGTSRSYGIQVARLAGLPEGVIERAKEILHNL
                     EQGEFTEAGVPKLAISKKRKLAWETHQRTLFQSPPDPIREALRKIDPNRLTPLDALNI
                     LSDLKSRLSDSE"
     gene            complement(22388..23035)
                     /locus_tag="VEL68_05240"
     CDS             complement(22388..23035)
                     /locus_tag="VEL68_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35404.1"
                     /translation="MKNLILAGLLFVLLPSLAFSQEKVEAPVWNVLDKWVFTGNGSIE
                     VVKADQSGYILKFSEGKCIFESQGCNTILFEKSSLNRMNVLDGDKSRKYGEGLSKILN
                     FPFSKGKQWKSGYSSTESGPYHRVFYDYSEIYKVLGWEDIAVQAGKFKALGVEHRRKI
                     IGSPGQWSSVGEEIINQYWYSPDVKYFVKCKYDKDWMKGKKEIFDWELTSFQLKK"
     gene            complement(23167..23643)
                     /locus_tag="VEL68_05245"
     CDS             complement(23167..23643)
                     /locus_tag="VEL68_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ35405.1"
                     /translation="MYTSIFRVGMVGVLFMVLLIPSGAFAQTKELPGFRLFDSQEFAK
                     MANPNKDKFYFTEIVNEKMKAKHLNGIFVILPPPSTGAKLAYHYHKDRESILYFISGT
                     GNEIVDGVKVPIKAGDVIFILPGVKHTIENTSDKEIRYIEFFTHPPVAADWVKVEK"
     gene            complement(23869..>25094)
                     /locus_tag="VEL68_05250"
     CDS             complement(23869..>25094)
                     /locus_tag="VEL68_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HXZ35406.1"
                     /translation="LHADLAIKFVTHVGSLLTQSFAHVAKAHAMHELGNHKEAADQLA
                     QASRTCDPSKNFLLKFFILIAEARFAFAQGKEASGLSFLRDALAIGKEEGITETYVDS
                     PSAMSLLCARALEARIEVEYVREIIRKRNLTLDEPFYHLENWPWPVKILTLGRFELWK
                     DGKPIQFSRKVQQKPLSMLKVLIALGGREVKEEMMADILWPEADGDAAHRSFISALHR
                     LRQLAGCEKAFQFKEGKLTLDKRYCWLDVWALENIWRQADTQRQEGRIDASFQLTEKA
                     IGVYRGAFLGGEPEQPWMVSLRERLRSKFLGCVNRLGQHWEQTGQWEKALECYQRGLD
                     ADDLAEEFYQNLMTCYLRLDREAEARAVYHRCRKILSSALGIEPSAKTQAIYKSLLEK
                     TSSEGEMKTKPQAPT"
BASE COUNT         5855 a         6696 c         7138 g         5405 t
ORIGIN      
        1 cgaatctggc tgccccaggc gatgtccttt ttggcgctgt gaagggcttc ggccttttct
       61 tcctgctccc gcaatttcag ctcgtagagg cgcgatttca gcactttcat ggccatggcg
      121 cggtttttgt gctgggaccg ttcattctga cattgaacga cgatgcccgt aggcatgtgg
      181 gtgatgcgaa ccgccgactc ggtcttgttg acgtgctggc ctccagcccc actggatcgg
      241 aaagtgtcca cccggaggtc ttcttccttg atctcgacca cgatatcctc ttcgacttcc
      301 gggtagacaa agacggaagc aaatgaggtg tgacggcgtt tgcccgcatc gaagggggag
      361 atgcggacca gacggtggat tcccacctcc gccttgaggt agccgtaggc gtactccccg
      421 gtgacctcga aggtggcgct cttgatgccg gcctcttccc ctgccagagt gtcgatgact
      481 tcggttcgga atcccttccg ctcagcccat cgcaggtaca tgcgtaacag catgctggcc
      541 cagtcctggg cctcggttcc cccggccccg gagtggatgg agacgatggc gttgttccgg
      601 tcgttttccc cccccagcat gcgcttgaat tccgccgcct ggaggtcttt ctccacctgg
      661 ttcactttgg ccgagacttc tttcagggtc tcttcgtcat tctcttcctt ggccagctcg
      721 aagtaccctg tggcctcctc catctcggcc ttcagcttct cccaaagctc cacttcggcc
      781 cgcaaggcag tcctctcctt cagggtcttc tgcgctcctt cattgtcgtt ccaaaagttg
      841 ggcttgaggg tttcctgttc gatctcggca aggcgggcga tcttggtgtc taagtcaaag
      901 atgacctcga agggcctgaa ctttctcttc caggagagga atcttactct cgatttcgtt
      961 ccacacggtc ttcccttcta aaacgaatgt agatcaaaaa caggatgatg tacccaaggc
     1021 acaggtaggt gaaaaggtcg ccgaattggg tgtaaaagct ccatcgattc tttatatata
     1081 tattatcgaa aagaatggcc tttgtaaaga tgtccgaggc ttgcaggatt cgtccgctgg
     1141 gatggatgaa tcccgaaacc ccggtattcg cggcccgagc cataggaacc cggttttcca
     1201 ccgcccgtag ggcagccatg gagatatgct ggtacggggc ggcggtgtgc ccgaaccagg
     1261 cgtcgttggt gatgtttacc aggaattggg ctccccggtc cacaaactgc ctggtcaagt
     1321 ccggcaggat ggcctcgtag catatgagaa ctccgagctt accctgggaa aaatccaggt
     1381 cccgcacttc ttcccccgac tgaaaatccc ccatggcccc gatgatgtcc cgggtgaacc
     1441 cgagaatgcc ggagagaggg gcatattctc cgaaaggaac cagatggatt ttgtcgtagc
     1501 gtccggttac tttcccctcc ggggagataa ggaaggcgct gttgtaataa cgggtttccc
     1561 ggcccttccg gtcataggcg ggcgagccga agagaaggtg ggttttcatt ttctgggcca
     1621 ggtcgagaat gcgggattgg tagggaaagc tgtcctggaa gaaaaagggg gtcgcagtct
     1681 ccggccagat gaccaactgg ggaagggttg cctgggtccg gagggtgagg tcggtgtaaa
     1741 tcctcatggt ttcttcctga aatttgggtt cccatttcac gtcctgcggg atgttcccct
     1801 ggacgagggc gatccggacc ggggtccctt ccttgctttc cgggctcatt ccggccagtc
     1861 gccaataccc gtagccggcg gtcaggacca ggaggaaacc agcggccagg atttctccgc
     1921 ggaccagcct ccattttcga tcagcgaagg cttccaggag gcggtacccg gcggcgttga
     1981 tcagaaccag cagaaaggat accccgtaca ctccggtaat gtcggcgatc tgcaccatgg
     2041 gaagagtgag atattgggag tatcccaaaa gctcccaggg aaagccggaa aataagaagc
     2101 cacggatgta ctcaagggag acccagagag gaggtgcgag aatggtttcg ggaatttcca
     2161 cccgcgcttc aacccatcgc agggcgaagg caaaggcgga aaaatagagt gcgaggtagg
     2221 cggtcaggag gaggaggaag aaaacactga ccgctcccgg caggtggccg tactgggtaa
     2281 gcacgttgta gatccaggga aggaggccgg tgaagaaaga aaagccgcac acaaacccca
     2341 gaagcgcggc ttgggaagga gacgcccggc ggatggacca gaaaaagggg attagagcgg
     2401 cccaggcgac gatctccgcg ttgaacttgg gaaaggccag aatcaagaga agaccggaaa
     2461 gggcggccag gaaaagggcg gggagcctca tgatcgctga agatcacccc ccaatcttga
     2521 ccatggggag atggcacctc ttcatcacgc cccccatggg accgtgatga gggggcttgg
     2581 taaggaggga ttcgtccagc ttcctctcca gttcctgatc agtagcaccg gagcgaagga
     2641 gggtttttaa atcggtcaac gcttcagaaa aaaggcaggt cttgagcttt ccgtcggcag
     2701 tgagacggag acggttacag gagccacaga agtgctcgct taccgggctg ataaaaccga
     2761 tctccccctt ggccccgggc agcttgaaga ggcgtgccgg ccccccgtgc ccgtttccac
     2821 tttcggccag gggaacaagg ggagaggttc tttcgatccg atccttaatc tcccggcagg
     2881 agatgcagtg ctcgggcttc cattcgctcc ctgctccgat gggcatgaat tctatgaaac
     2941 ggacttgaaa gggctccttc cgggtgagat gagcgaagtc ttcgatctcc ccgtcgttga
     3001 acccggcaat ggccaccacg ttaatcttga tcggattcat cccctcgcga cgggccgtct
     3061 cgatcccttt ccacacctga ttcaggtccc ccccccgggt gatctcccgg tatttcccgg
     3121 gatcaaggga atccatgctg atatttacgc gtctcaaccc tgcccgacga aggtcccggg
     3181 cgaactcccg gagaaggatg gcattcgtgg tcatggagag gtcccggacc ccgggaaggc
     3241 cggagagctt ttcgaccagg ttaaccacac ctttgcgcac caggggttct ccgccagtga
     3301 tccggacctt gtcaatcccc cgttttacag ccaagccagc cagtcgcaga atctcctcat
     3361 aggagagaat ttccgagtgg ccaaattggg agaccccctc tttgggcatg cagtaacggc
     3421 agcgaaggtt gcaccgatcg gtaatggata tacgcaaata gttcaggcgc cggttatggg
     3481 aatcgacgag accgttcatt ttgagactga aaaaagaagg aaccgcagtt tcaggaatga
     3541 agaaaactct atttttctca tcgtcgcttg gatgcttcta gcttcgaccc aaatatctat
     3601 tttttattat accttttcag gttatttttg aaaagcggaa aaccctcccc ctttccttct
     3661 gcaagatcaa tgtttattcc tggaaccctt gcccccgggg cacaagagaa gggctctcaa
     3721 gagggaaagc ctctaagaag gggaaacctt gcccaccccc aattcttaaa aaagagtcct
     3781 tcgaaaataa gacctctgga aagcaggggg ggatctcatg gggttcaggg cggagaaatt
     3841 tggatcatca tcctgaggcg gtggttcttc catgggatcg ggcctgcgct gggggattga
     3901 aacggaaaca tgctttggct ctcttttctg cttggaggta tcttctctcg ggcctctccc
     3961 tgcggaacat agggaggagt ttcatggaag gataagcacc cgccgagcct gatgatccaa
     4021 attcctccac cctgaacccc aacaagcacg aaaaactggg ggtgggcaag aaggggaaac
     4081 ccgttgattt tcgaatcgca atgggttaca taaagaaaaa gtctcgaatc ggcttagaga
     4141 ttcgactgga aatgggaggg atagctggag cgagaaaaag gattggccat tatcaccatc
     4201 gatggtccag cgggagcggg caagagcacg gtcagccgaa tgctggcccg ccggctgaat
     4261 tacctctacc tcgacacagg agccatgtat cgggccgtag cccttcaggt actcaaggaa
     4321 aggattgacc cgggcgatga aaaggcgctg gaagggctct gcaaaggaat tgccatcgct
     4381 tttgccgaag attcccgggg gcagcgggtc ttctgccagg gtgaggacgt gactgaaaag
     4441 atccgggaac cggaggtcag ttggatggcc tctgccgttt ccatgaaacg gccggtgcgg
     4501 gaagccatgg taactctcca gcgcaggatg ggagcccagg gaggattggt ggccgagggg
     4561 cgtgacacgg ggaccgtagt ctttccccgg gcccaagcca aattctttct ggtcgctgat
     4621 ccccgggaac gagccctgag gcgtttccgc gaactgaagg ccaaggggaa agacgcgaag
     4681 ccggaggagg tggaagagga tgtgcgaaag agggatgttc aggactcttc ccgcgatctt
     4741 gcccccttga gacccgcaga ggatgcccac ctcatcgatt caaccggact caccccggaa
     4801 gaagtggtgg atagaatgct ggaggtcctg gggcgatcct tttcttgagg gggaaccccc
     4861 cctctgccga gaaactccgg gagtccgaaa tcacccggtt cattaattat ggttgtggtt
     4921 ttttttcaat atgatataat gcgggaccag aagaccggaa caacgtgtgg ggggtactgg
     4981 aatgcttgac aaagaggagc agtttctaac cgcaaaaccg gaggatcggc ctccaaccga
     5041 agcagcggaa gaatcctacg agaacaaagg gaaacccgaa gaaggggaaa gctttcagga
     5101 gctttacgaa gagagccttc atgaaatcgc cgaaggggaa gtggttcacg gaagggtgat
     5161 tcatgtgggg ccggaattcg tgaccgtaga catcggcttc aaatccgaag gtcagctctc
     5221 gctgaatgag tttcgccaga aggacggaac gctgtcggtg aacatagggg acgagattga
     5281 cgtattcgtg gagaggaaag aatcggaaat cggaatggtg accctctcca aggaaaaagc
     5341 cgacaaattc aagttctggg aggaaatcag ccgggcctgg aacgaggatc aggtgatcga
     5401 gggcaagatt atttcccgaa ttaaaggggg actgaccgtc gatatcgggg ttctggcttt
     5461 tttaccgggg tcccaggtgg atattcggcc cattcgcaac ctggaaaaac tcatcggcag
     5521 cactcataaa ttcaagatta tcaagctgaa ccgtcgccgg ggaaacgtag tcctgtcccg
     5581 gcgcatactt ttggagaagg aaagggaatc catccgccag aagaccctag aggttctgca
     5641 agaaggccag attgtggaag gggtggtaaa gaacatcacg gactatgggg ccttcatcga
     5701 cctagggggg atcgacggcc tcatgcatat taccgacatg tcgtggggac ggattaatca
     5761 tcccgcggat attctcacgg tagggcagaa gatcaaagtc aaagtcctcc aattcgagcg
     5821 gcaaaatcaa cgggtttcat tggggctgaa gcagaccact ccggacccct gggacaatat
     5881 tgccgcgaag ttccccgtgg gaggccgggt caaagggaag gtcacctcca tcaccgacta
     5941 cggagccttc gtccagctct tggaaggggt ggaaggtctg gtccatgtct ccgagatggc
     6001 ctggaccaag cgggtccggc atccctcgaa gattctttcg gctggcgatg aagtggaggt
     6061 tatggttctc gacgtggact ccaaccagaa gcggatttcc ctggggttga aacagacgag
     6121 tccgaatccc tgggacacga ttgccgagcg ctatccggtg ggaaccaaga tccaggggag
     6181 gatcaagaat atcaccgatt tcggaatttt catcggcatc gatgaaggga ttgacggcct
     6241 ggtccatatt tccgatattt cctggacgca gcggttgaaa catccttccg agatgttcaa
     6301 aaagggccag gaggtgcagg ccatcgttct gagtatcgac cgagagaacc agcgcttttc
     6361 cctggggatc aaacagattc agaagaatcc ctgggacgat atccatcacc ggtaccgtgt
     6421 ggggcagatg gctaaaggaa aagtcacgaa cgtgaccaag tttggagcct tcgtggaact
     6481 ggagccgggg atcgaagggc ttgtgcacat ctccgagatc agccaccaga gagtggagaa
     6541 ggcggaagac gcgctcaagg ctggaagcga gatccaagcg gtggtcatca acgttgatcc
     6601 gaggagacat aaaatcggcc tcagcatcaa ggatgtagag cgataccaga ctcagcaggc
     6661 ccgcaaagaa actgcggctg cctatgaaga cgacacttct gattttgggc gaatgctgaa
     6721 ggaaggactg gagaggagag cccgggaggc gaatagttcc agggaatcgt gatcggcaga
     6781 tgaaaaaaca ccctattttg ttgggactta cggtcttggg ggtgctcctg ggccttttct
     6841 tggtcagtat ttgggtctta tcctactttt ccaaccctga agagagcttc tggggcgggg
     6901 aaaaaatcgc cgtcatcgaa gtccgggggg tcatcctcga cccccagccc atcgtagaaa
     6961 agctggtcaa actccggaag aacgagaaaa tcaaggccat tgttctgcga atcgactcac
     7021 ccggcggggg ggtggggccg gctcaggaga ttcacgccga agtgaagaag gcccagaagg
     7081 agaaaaaggt tgtggtctcc atgggctcgg tggcggcctc cggcggttat tacatcgctt
     7141 gcgccgcgga tcgaattatc gctaatccgg ggtccatcac cggctccatt ggggtgatcg
     7201 tggagagcct caacgtggaa gagcttctgg gcaagctcgg cctccgctcc gtggtggtga
     7261 agagcggcaa gcacaaggac ctgggctctc ctatgcgccc catgaccgct gaagaccgga
     7321 ggctgcttca gggagtaatt gacagcgtcc atgaacagtt catccgcgct gtggccgagg
     7381 gccgcaagct ccctttggag aaggtccgga aactggcgga tggaagaatt ttttccgggg
     7441 accaggcgcg caccttggga ctcgtggatg aactggggaa cttggaggac acgattgccc
     7501 tggccgccca aatggccgga atccgtgggg agcctgaaat catctacccg gagaaaaaga
     7561 gattctccct cttcgatctt ttaatccaag aaaccgtcca gaagatcgtg gaaagcctga
     7621 aagagagcac tccgcccttg aatttcctct attactggcc cttcagcgga tgatttctct
     7681 cccatgcccc tggctcgatt ggcctccatt gacgtagggt ctaataccac ccgccttctc
     7741 ctcgcccagc cattccccga ccagaccttc cagcctttgc gggtggagcg aatcatcacc
     7801 cgcctggggg ggaatttttt ccccgggaaa aagctcaaca aaatctccat agaaagaacc
     7861 ctgaaggccc ttcgctcctt tgccgaactc ctggaaggga tgggggtgaa aaaggtcatt
     7921 gccgcgggga ccggggtctt gcgccaagcc aagaaccgaa gcgcctttgt cagagcggtc
     7981 agggagcaga ccggtttatc ccttcgaatt ctttcgggtc aagaggaagc ccgagcgacg
     8041 ctcaggggag tccttgggtc cctcagggat cgaaacacac cccggctgat cactgatgtg
     8101 gggggagggt ccaccgagat tatatggatg gaaggacgga ctcttcgaga gaccgtcagc
     8161 ctgaacctag gggccgtggg attgaccgag aaatttttat ccaaagaccc gcccagctct
     8221 accgaaatgg aagccctcaa acgattcgtc cattccgctc tccaaagggt gctgagacag
     8281 tgggaaaggg gagggctcca tacccaagcc cttcacccct gtttggtagg aacggcaggg
     8341 acggctacca cgctgggggc catcgacctt tccctttcgg tttacgaccc acgaaaggtc
     8401 aacggacacc ggatttccat ctccaagctc aggagaatgt accgccgtct ctccactctg
     8461 cccttaaaag aacgggtcaa actccccggg ctggaaaagg ggagagaaga cctgatcctg
     8521 gccggctcat ccatcctgtt gaatctaatg gaagtcttta gacgttccgc cctggaggtg
     8581 atcgattcgg ggctgctgga agggattttg ctcgaaggaa taggggaaat gtagaattct
     8641 tgacggggaa gggaaagcgc ggtttatcat ccttgacata tacccaggcg cataatagat
     8701 ttattattat taatcgtcct gcaggagccc gtcatgattc ccgagaacct aaccgaaagc
     8761 cttacctttg acgacgtcct tctactccct tcagagtcgt ccattcttcc ccgggatgtg
     8821 gacgtaacca cccagctaac ccgggcgatc cgcctgaata tcccttttct gagtgcagcc
     8881 atggataccg tcaccgaagc ccagatggcc attgccatgg cccgggaagg ggggatcggg
     8941 attatccatc gcaacatgtc cgtaaccgcg caggccgcgg aagtagacaa ggtcaagaag
     9001 tcggaaagcg ggatgatcgt cgaccccatt actatggacc cggaccaaaa gatatacgag
     9061 gccctggaga tcatgaagaa gtaccgcatc tcaggggtgc cggtgacccg caaggggaaa
     9121 ttggtgggaa tcctgaccaa ccgggacctc cgtttcgaaa cccagctcga ccagcccatc
     9181 tccgcggtca tgaccaagga gagactggtc actgcccatc ccggaatcac cctggaagag
     9241 tccaagcggc tgctccacaa aaaccggatc gaaaaactcc tggtggtgga tgagcagaat
     9301 aacctgaagg gactcattac cattaaggac atcgagaaga ccatcaagta tcccaattcc
     9361 tgcaaggatc agctgggccg actgagggtg ggagcggcgg taggcccggg gaccgatcgg
     9421 gacgcacggg tggaagccct gctcaaggcg ggagcggacg taatcgtcat cgacacggcc
     9481 cacggtcatt ccctcaacgt ccttgaatcc atccgggcgg tcaaaaaatt gtttcccgat
     9541 tgccagctcg tggcgggaaa cgtggccacg gcggagggga ccgcggccct cattcaggcc
     9601 ggggtggacg cggtcaagat cggggtgggg cccggttcga tctgcaccac ccgggtcatc
     9661 gccggagtgg gggtcccgca gatcaccgcc atcgcggaaa gcgcccgggt cgcgcaaaag
     9721 taccacattc ccatcatctc ggacggcggg atcaaattct cgggagatgt taccaaggcc
     9781 ctggctgccg gcgccgacac ggtgatgatc gggagcctct ttgccggcac tgacgagagc
     9841 ccgggggaga tggtcctcta tcagggtcgg agttacaaga tgtaccgggg catggggtcc
     9901 atcgaagcca tgaagggcgg aagcaaggac cggtacgggc aggatgatgt ggagtcggag
     9961 atgaaactcg ttccggaggg gattgagggg agggtcccct acaaaggccc gatctcctcc
    10021 agcatctacc agcttctcgg gggtcttcgg gccgggatgg ggtatgtggg gtgccgcacc
    10081 ctgaaagcat taaaagaaaa gagccggttt accaagatta cctccgccgg cctgcgggag
    10141 agccatgttc acgacgtgat catcaccaaa gaggccccca actacagccc gggcgaactg
    10201 agttcctgga aggagtcttg agattgatgc aggtgaagga ttttgaggga cggatcctca
    10261 ttctggatta cgggtcccag tacacccagt tgatcgcccg gcgggtgcgc gaagcccacg
    10321 tgtactgcga aatccatccc tttcatatga gcctggaagc cgtccgggat tttcagccca
    10381 agggaatcat tctctccggc ggcccgtcca gcgtgtacga aaaagactcc ccttggaacc
    10441 cgccggacct cttccgtttg agtatcccca ccctggggat ctgctacggc atgcaactca
    10501 tgacccatgt gctgggcgga agggtggcca aatcaagcaa gcgggaatac gggcggagcg
    10561 aactgttcat cgatgatgag cgggacctct ttgccgggct ggagagcggg aaagccattc
    10621 aggcctggat gagccatggg gaccggatcg aagtccctcc cgagggattt caggtgatcg
    10681 cccattcgcc caattccccc gtggcggcca tccgccatca ggacctctgt gcctacggcg
    10741 tgcaattcca tcccgaggtg gtccataccc ccctgggaaa gcaaatcctt caaaattttc
    10801 tcttccgcat ctgccactgt cccccggatt ggaccatgga gtcctttgtg atcaagaccg
    10861 tccagttcct gaaggagcag atcggaaaag agaaggtgat ctgcggcttg agcggagggg
    10921 tggactcatc ggtcgtggcg gttctcctgc ataaagcggt ggggagccag ctgaccagca
    10981 tttttgtgga caatggcctg ctgcgcaagg atgaagcgag aaaagtgaag ggtctcttcg
    11041 gcttcaacct gggcttgaac ctgcgggtcg tggaggcggg cgaccggttc ctggacaggc
    11101 tggccggcgt gatggacccg gaacagaagc ggaagatcat cggcaacgag tttatttacc
    11161 tcttcgagaa agaggcccag aaagccggag gcgccacgta cctagcccag gggaccctct
    11221 accccgacgt gatcgagtcc gtctccttca aggggccttc ggccaagatc aagagtcacc
    11281 acaatgtggg cggactcccg gagcgcatgc atcttaagct cattgagccc ttaaaagaac
    11341 tcttcaagga cgaagtgcgg gccgtgggcc gggaactggg ccttcccccg gagctcatcg
    11401 aacgccatcc ctttcccgga cccggactgg cggtacgcac cttgggcgcg gtgaaccagc
    11461 aagacctggt tctcctgagg gaagcggacg ctatcgtgga agaggagatg caccggacag
    11521 ggtggtatac gagggtctgg caagcctttg ccgttttgct cccggtatgc acagtgggag
    11581 tcatgggcga cgagcggacc tatgaacggg tcgctgtcct tcgcgtggtc cagagcctgg
    11641 atggaatgac cgccgactgg gtgccccttc cggccgaagt cctgggaacg atttccaacc
    11701 gcatcatcaa cgaggtccgg ggaatcaacc gagtggttta cgacatctct tccaaaccgc
    11761 ccagcacgat tgaatgggaa taaggcgcaa ggcgcccaag aagttcgaaa ttcggagttc
    11821 aaagttcgga gttgaattcg cttttattta tcttaatttt agtcactcaa ggcactccgc
    11881 actctgggca ctctaggcac attttatgaa acacgcggat ttcgtccatc tccaccttca
    11941 tacccaatac agcctcttgg atggcgccat ccggctcgat gatctcttcg agaaggccaa
    12001 gagctaccgg atgccggccc tggccatgac cgaccatggg gccatgttcg gagccatcga
    12061 attttaccag caggcccaga aaaccggcct caagcccatc atcggatgtg aggtctatgt
    12121 ggccccaacc agccggtttg ataagaatcc aaaaggtccg tccgaggcct cccaccacct
    12181 gattctcctg gcaaagaacc tggcagggta ccgaaacctg atgaaactgg tctccctggc
    12241 ccacttcgaa ggattctatt acaagccgcg ggtggataag gaactcctgc aggcgaacaa
    12301 cgagggactc atcggcctca gtgcctgtct tcagggcgag gtgccctatt acctgaccgg
    12361 gggagacaaa agcagggccc tccgggcagc cgatgaattg aagtcgatct tccccgacca
    12421 tcgcttttac ctggaaatcc aggagaacaa gattcccgag caaatgaagg tcaaccaagg
    12481 cctgatggag atttcccggg aattgtccct tcccctagtg gccaccaacg actgtcatta
    12541 cctgagaagg gaagacgccc aggcccacga agtcctcctc tgcattcaga ccgggaaaac
    12601 tctgaaagac gcagaccgga tgaaattctc gaccaacgaa ttttttttcc gttcggcgga
    12661 agagatggag gcgctttttt cctactgccc ggaggcggtg cggaacagcg tcgaagtcgc
    12721 cgaacggtgc aacctggact tgaagctcag cgaactccag ttcccccagt ttcaggttcc
    12781 cccgggagag accctggaca gtttcctgga aaagaccgct caaaagggcc tggaggacag
    12841 gctgatcgag tggaaggcgc tcggccaatc cctccccgtg aacatggagt tctaccgaca
    12901 gcgcctgcaa aaagaagttg gcatgattca gaaaatgggg ttctcggggt atttcctgat
    12961 cgtctccgat ttcatccgtt acgcgaaatc caaaggaatt cccgtggggc cgggccgggg
    13021 gtccggtgca ggatcgcttg cggcctactg cctgggcatc accgagatcg accccctggt
    13081 caacggactc ctcttcgagc gattcctgaa ccctgagcgg gtgagcccac cggatatcga
    13141 catcgatttt tgcatccagg gccgggagga ggtcatccag tacgtccgga gcaagtacgg
    13201 ccaagaccat gtggctcaga tcatcacctt cgggaaaatg caggcccggg cggtgatccg
    13261 ggacgtgggg cgcgccctgg atatgcctta cggtgaagtg gaccgcatcg ccaagatgat
    13321 ccccaacacc ctcaacatca ccctgcagga ggctcttcaa caggaacccc gcctgaacga
    13381 gctgggcagg agcaatccgg aggtaaaaaa gctcttaacc cttgcccagg ccctggaagg
    13441 attgccccgt catgcctcta cccatgccgc tggggtggtg atcagcaacc gtccgctggt
    13501 cgaagtcgtc cctctgtacc gggggcagca gaatgaaatc atgacccagt accccatgaa
    13561 ggacgtggag aaaatcgggc tgatcaagtt cgatttcctg ggcctgaaga ccctgacaat
    13621 gatcgaagat gtgctcaagc gggtggaagc ttcccgggga gagaggctgg acttgcgccg
    13681 gatctccctc acggaccgca agacctatga actcctcagc tccggggaca cctcgggggt
    13741 gtttcagctg gaaagctcgg ggatgaaaga cctggtcatg cgcctgcttc ccgaacgttt
    13801 tgaggacctg gtcgcccttc tggccctata ccgccccggg cctctgaaga gcggcatggt
    13861 ggatgacttc atcaagcgca agcaaggcaa gaccctgatc acctatgaaa tccccgagct
    13921 gaaggagatt ttggaagaga cctatggggt cattgtctac caggaacagg tcatgaagat
    13981 cgccaccaaa ctggccagct tcaccctcgc ccaggcggat attctgcgtc gggccatggg
    14041 gaagaagaat gtggaagaga tggccatgca gaagaagaac ttcatcgaag gtacccagag
    14101 gggcaaaatc gcggggaaga aagcggagaa gctcttcgac ctcatggcca agtttgccga
    14161 gtacggtttc aacaaatctc acagcgcggc ctacgctctg gtcgccttcc agacggcctt
    14221 cctcaaagcc cattatcccg tggagttcat ggccgcgctc ctctcttcgg aaatgggaaa
    14281 ctcggataag atcctgcgtc acctgggaga atgccgggaa aaaaagatcg aagtgctccc
    14341 tccggacgtg aatgaaagcc agcaggattt cacggttgtg agcgggaaga ttcgcttcgg
    14401 cctggccgcg gtgaagaacg taggcttggc cgccatccaa tcgatccttt cggcccggga
    14461 ggagaagggg aattttacct ccttggctga tttctgcctg aaggtggacc tgcgcaaagt
    14521 caacaaacgg gtcatcgaga gcctgattaa aagcgggtcc ttcgaaagcc tgggcttctc
    14581 ccgggctcaa ctcctggccg gactggagga ggccatggaa tgggcccagg gggtggaacg
    14641 ggagcgggcc aaccatcaga tcgccatgtt cggagggatt cggggaggga agcgcaggga
    14701 cgagccccgt cttcccgagt tggccccttg gcccgaaagc caaagattgg ccgcggagaa
    14761 ggaaaccctg ggtttctacc tgaccggcca ccccttgagc agttatgccc cgaccctcca
    14821 gcggttcacc tccatggaca cgctcagagt ccaggaggcg gccgacgggc aggaagtgat
    14881 cctcggggga gtggtcaact ccctcaagga gatcaacacc aaaaagggag atcgcatggc
    14941 cttcgtcagc ctggaagacc tgaacgggat ggtggaggtc attatttttt ccgagctcta
    15001 taaaaattcc tccctgctgc tgaagagcga agaccctctc ttcatcaagg ggagagtgga
    15061 tgcaggggag gagagtgtaa agatcatcgc cagcgaggtt cttcccttcg aacaggccgt
    15121 aggcaagctg accaccagcg tccatctccg ggtgcattct gaaggcctgc ggcggcagga
    15181 cctggaggct atgcgggaga tcttccaggc ctgccggggc agcagtccgg cctatctcca
    15241 tttgattctt ccaggggaga aggaagcggt catcgaccta ggcgaagatt ggaagctgaa
    15301 ttccaccgac cagttggtcc gccggatacg ggacctgctg gggtatgagg cggtcagttt
    15361 tcaagcttag gattattggc ctttttcccc tcttcgccct ttcgcctttt ccccttgatt
    15421 taaggtctat ccgaatggct ctttattatc tggacttcga aaaacccatt gccgagatcg
    15481 acaaaaagat tgccgagctg aagacctttt cctaccatcc ccatcccaga atggccgaag
    15541 aagtcgccag tctggaaaaa cagcttcaga gaactctgac caaggtttat tccaacctct
    15601 cccggtggca gcggacgcag ctttcccgtc atttcaaccg tcccaccttt ttggagtatg
    15661 tggaacaggt attttccgaa ttcctggagc tgcacggaga ccggaaatat ggggatgacc
    15721 cggcgattgt ggggggggtg gccaagtttc agggggaacc ggtcatagtt gtggggcatc
    15781 agaagggccg aacggtgaag gaaaagatga gccgcaattt cggaatgccc aacccggagg
    15841 ggtatcggaa agcccttcgg ctcatgaaga tggctgaaca attcggaaaa cctctgatca
    15901 cgatgatcga cacccccgga gcttatccgg gacagggagc ggaggaaagg ggacaggccc
    15961 tggccatcgc cgaagacctt caagccatgg ccaccttgcg cacccctatt atctctgtga
    16021 tcatcgggga gggcgggagc gggggcgctt tggctttagg agtgggagac cgcctcctga
    16081 tgctggagaa tgccacctat tcggtgatct cccccgaggg ttgcgcggct attttatgga
    16141 agggtgaaac cgaaaaggaa aaggccgcag aggctctgag gggaacggcc gccgatctat
    16201 tggaactggg ggtcatcgat gaagttcttc gcgaaccgct gggaggagcc caccgcaatg
    16261 ggaaggagat ggcccgcacc ttctcggaat ctctggcccg tcacctgaaa ggactgcggg
    16321 gaatccccat ggaagacctc ctgcagcaga gatacgaaaa atatcgccgt atgggtcggg
    16381 tgctcggggg gtaggagttc tgccgtggaa gaaaaacccg gaggtttccg ggaggcctac
    16441 caggaaagaa aataccggaa ccttcttccc gatcaaggaa tggtctcctt tcaggtgatg
    16501 gtcaaggaga cggaccttta tatcaaggcc tcacgcaacc tggccgagcc tgcccgcgag
    16561 tccgtcctcc gctaccgtca ccagctcgaa agctacatcc atcttcatcc ctcatttctt
    16621 cactccctga ccccttggcc cacggatgat tttgcagcgc ccatcatcca gagcatgatc
    16681 cgggcaggtc agggggcggg ggttgggccc atggctgcgg tggcgggagc catggccgaa
    16741 tcggtaggtc gggacctgtt aaaggaggct cccgagatca ttgtggaaaa ttggggagac
    16801 atcttcctcc gttcctcccg ggggattccc gtggggatct ttgcgggcaa ttctccttta
    16861 agcctccggg tcggcctttt ggtccctgct tccgaagacc cttcgggagt gtgcacttcg
    16921 tcgggaacgg tggggccatc cctcagcttc ggccgagccg acgcggtttg cattctctct
    16981 ccatctgcat ctctggcaga tgccgcggcc acggcagtgg gcaatcgggt gcggtcctct
    17041 accgatctgg aaagggggct ggaacaagcc aaagagattg tgggggttgt cggcgcagtg
    17101 atcatcctgg gagacaaact ggctgcttgg ggagagatcg aactgatcga tttgaaaaga
    17161 taactgggtt ccttgcctaa tttccttttt ttttgcgagc gaaaagaata taataagggc
    17221 cactgggaac caggtccgag gaagcgggat cgaagtcagt tgattcgcat cggagtgatt
    17281 tccgataccc atttggggca gccgaccgaa gaattgaaag ccctgctaca agggtctttc
    17341 aaagaggtgg agacgatcct gcacgccgga gacctaaccg aactagccgt tttggaagct
    17401 tttgccggca agaacctatt ggccgtctgc ggaaacatgg atgctcagtc ggttcgaaag
    17461 cagcttccca cccatcgggt tttccaagca ggaaaattca ccatcggatt ggcacacgga
    17521 tggggagggc ctcaagggat cgaggagcgg atcgccaagg aatttggaaa ggtggattgc
    17581 attgtttacg gccacacgca taccccctcg caaagggagc gggaagggat ttttttcttc
    17641 aacccgggcg ccttccacgg gaaaaggggt tggagctcaa ggagcgtggg cattttgaac
    17701 ctgggcgggg ggatttcggg ccaaatcatc cacctgtgag acccaaagga gaggggaatg
    17761 attgaacgtc tcttcgcccc gtggcgaatg aattatattc tggcggacaa gaagggcaaa
    17821 aaatgcatct tctgccctat ggatggccat tcagaccgag agaggctgat tcttcaaagg
    17881 gcctcccact ccctggtgat gctcaaccgc tatccttatt cctatgccca tctcatggtg
    17941 gctccgatgc gccatgtaga ccaggtgcag aatttaaata aaaaagagat ggccgatatc
    18001 ctcttttccc tggggcagtc catcgagatt ctgagggagg cttttcatcc tgatggtttc
    18061 aacgtgggaa tgaacctcgg gcaggtggct ggggcagggg ttctccatca ccttcacttt
    18121 cacatcgtgc cgcggtggaa cggggatacg aacttcatgc ggctctcgga gaggtccggg
    18181 tcatccctga gcacctgaag gaaacctacc gtaggttatt gcccttcttt cggaagatcg
    18241 aggaataggc ggagggagta tggggctagc tcgtgcgatc attttttttt ccctcttggt
    18301 gttgggaatc gggtttgcca tcctgaacga tgggcctgtt tccctcaagt actactttgg
    18361 cttggagact catcctcttc ccctgttcct ctgggccctc ctttttctcc tgctggggtt
    18421 gatcctgtca ggattgtggt cctttttctc caaaatcgga ctccattccc ggattcggca
    18481 ttcccaaaga gcgatcgccg accttgaaag ggaacggaac cgcttggcgg aggagaggaa
    18541 aagtctctga gtcggacttc gaaggccttc ctatacaaag cgacattgat ttgtgcaaaa
    18601 ctcgtcattc cctgcgaaag cgggaatcca ggaaagaatt ggattccggg gcttcgacgc
    18661 gagctcagcc gaacgtcaag cccggaatga cagacgcgat aagatttagg tcagcgtgta
    18721 tgaacgggtt tcgattccag gcaggcagaa aggagaagag gggagaaaga ggaacacgga
    18781 tttcctttcc ctcccccggg aaaaagacta cttctcgatt ttggcataaa ggacggggaa
    18841 gcctcccgcc tcgagggctt tcttgatggg ttcgaagtag aaggtcttca atttgaagac
    18901 caccagccgc tttccggttt catcggccct atccgtcacc acgctggtga tgttcacgtt
    18961 gtgttcttta atgatccggg tgatatcggc cagagccccg ggcttgtcgt ccaccataac
    19021 ttccagccga cttccttttt ccttcatccc cattacctcg atcaggatct ccatgacgtc
    19081 attctcggtg agaattccca ccagattgcc gtcttccacc acggggaggc agccgatttt
    19141 gttgtcgtga agtagaatgg cggcttcctc gatggaaata tccggggaga tggtgatgac
    19201 attccgggtc atgatctctc ccacggtgag cttgtccagc aaatagtgga gctcccgaat
    19261 ttccagggat gtggctggcg aagcctccgc cttgcgaata tccatgttgg taaccaaacc
    19321 aatcaatctc tttccgtcca caaccggcaa atgccggatg tttttctctt tgagcagctt
    19381 cttagccagg gtcatccggt catccttggt aatggtgacc agatcctttt tcatctttcc
    19441 tctaaccagc ataggaatcc ctcctgtccg ctgttgataa acaatctagg ttcagtctat
    19501 tgaatccgga aatggacgtc aagggaatta ttttaaaggg ttttaaaggc cggttgttgg
    19561 ttcctcgttt cttgttccag attaaaggaa ttttgtttta acctgcaacc agtaacccgc
    19621 aaccagcaac aagtccttcc tgcggttgaa ttttccgaat gagtaaggta acgtgaataa
    19681 gaggtgccga ggattcgcgc agatgaacaa cgttaccccg atgatccagc agtacttgga
    19741 agccaagagc aaatgtcccg atgccattct tttttatcgc atgggtgatt tttatgaaat
    19801 gttttttgaa gacgcaaaga tagcatcaga aatattagac attgctttaa cctcgcggga
    19861 caagagcaaa gaagactccg ttcccctgtg cggggtcccc taccattcgg ccacaggcta
    19921 catccaaaga ctagtcgacc ggggattcaa ggtggctgtc tgcgagcaga tggaggacgc
    19981 ttcccaggcc aaagggctcg tccgccgcga aatcatccgg attataactc caggaatggt
    20041 gctggaagac gagctcctcc agccgaaggc caacaatttc ctgatggcca tttccctggg
    20101 aggggaacat ttcggtctgg ccattctgga tgcctcgacc gccgattttc tcgcggcgga
    20161 agtgaatacc gttcaatcct tgcgggatga ggttctgcgc attgccccca aggaggtcct
    20221 cctggccgaa tcggccaaag gacaagctcc ggtgaaaagc ctgatgaagg cttatcccca
    20281 ggcgctttat agctccctgc catccgaagt ctttgatgag gagcgatcgc gggagcggtt
    20341 gaagtccctg ggcctttccc tcccggaagg aaaggagcaa tccctgcggg cagtgggggc
    20401 cattctattt tatgttcaag agactcagaa aacctcccct gtccatctct cacggctgga
    20461 attctaccaa gtccaggatt atctggtttt ggacgaaacg acccggagaa acctggagct
    20521 tttcgagaat ctccagagcc gcagccggaa agggtccttg atcgacatcc tggatgagac
    20581 ggttacgtcc atggggggac gcatgctcaa gcggtggctg ggctctcccc tgatgaatat
    20641 ggaaaagatt caggaccgac tggagaaagt ggccgagttg aaagaaaagg acctgcccag
    20701 gaaggccctc cgagagaccc tgcgccagat caaggatatg gaaagactct cggctcggat
    20761 ttccctggga gtggccaatg cccgggacct ggttgccctc cgggcttcgc tgaatattct
    20821 gcccgatgcg aagactcttc tttctcccat ggagtataag gggttcaaac cgtatctgaa
    20881 ggaaatggac gatcttccct ccatccggga ccttctggac cgcgccatcg aggactctcc
    20941 tcccttgtca ttgaaagagg gagggctgat caaaaggggc ttcgatgcca ctctggaccg
    21001 gtaccgggat atggggcggg agggaaaggg atggattgcc gacctggagg ccaaagagcg
    21061 gatgcggacc gggatttcct ccttgaaggt ccgtttcaac agagtcttcg gctattacat
    21121 cgaggtcacc aaagccaatc tctcctccgt tccttcccat tatatgcgca agcagaccct
    21181 ggcgaacgcc gagcggttca tcaccccaga gcttcaggag tacgagaccc aggtcctcca
    21241 cgccgaggag cagagcgaag ccctggaata ccagctcttt caggaagtcc tccaaaaagt
    21301 gactgctgag gttccccaaa ttcagaacag tgcccaggcg ctcgccgaag tggatgccct
    21361 tgccgccctg gccgaggtgg ccgagcggta tgcctacaac cgtccccagc tggatgaagg
    21421 ggacacccta gtcatcgtcg agggccgaca tccggtcctg gagcggctct cccaggatga
    21481 acggttcgtc cccaatgacc tttttcttaa tggggaggac cggcaaatcc tggtcattac
    21541 cgggcccaac atggccggga agtcaacgat catgcgccag gcggcgctga ccgttatcct
    21601 ggcccagatg gggagcttcg tcccggccaa agaagccagc gtatgcctgg tagaccgcat
    21661 cttcacccgg gtgggggcca cggatgacct ggtgcggggc cgaagcactt tcatggtgga
    21721 aatggaagaa gtagcccata tcctacgaaa cattactccc cgaagcctga tcctcctcga
    21781 cgagatcggc agggggacga gcaccttcga cggcctctcc atcgcttggg cggtaaccga
    21841 atacctccat gacagttgtc ccttccagcc caaaacgctt tttgccaccc attaccacga
    21901 gctaaccgaa ctggccatga ccaaaccgcg ggtaaaaaac tttcatgtag ccgtaaagga
    21961 atggaatgat cgagtgatct ttctccgaaa gctggtggag ggggggacca gccggagtta
    22021 cggcatccag gtggcccggc tagccggtct accggaagga gtaatcgaga gggccaagga
    22081 aattttgcat aatctcgagc aaggggagtt caccgaagcc ggggttccca aactggccat
    22141 ctccaagaaa agaaaactgg cctgggaaac ccaccagcgg acccttttcc agtctcctcc
    22201 ggacccgatc cgcgaggctc tgaggaagat cgatcccaac cgtctcaccc ccctggatgc
    22261 cctgaacatc ctgagcgact tgaaaagccg cctttccgac agcgaatagc cccggcagct
    22321 cgtagggcgg ttcgcgaacc ctggattttc aaatactgca gggggattaa cgattgagcg
    22381 gaaaaaccta tttttttaac tggaaggaag tcaactccca atcaaaaatt tcctttttcc
    22441 ccttcatcca atctttatca tatttgcatt tgacaaagta tttaacatcc ggcgaatacc
    22501 aatactggtt tatgatttct tcaccgacac tcgaccactg gcctggagag ccaattattt
    22561 ttcgtctatg ttctacccca agagccttaa attttcccgc ttggactgct atatcctccc
    22621 agcctaaaac tttgtatatc tcagaataat cgtaaaatac ccgatggtag gggccagatt
    22681 cagttgatga atacccgctt ttccattgtt tccctttgct aaaagggaag tttaaaattt
    22741 ttgatagtcc ctccccatat ttcctgcttt tgtctccatc caaaacattc atcctgttca
    22801 gactggattt ttcgaataat attgtgttac acccctggct ctcgaatata catttgcctt
    22861 ctgagaattt cagaatatag ccactctggt ccgctttgac gacttcgata ctcccattcc
    22921 cagtaaaaac ccatttgtct aacacattcc aaacaggggc ctcaaccttt tcttgagaaa
    22981 aggccaagga aggaagaagc acaaacaaca atcctgccaa tatcaaattt ttcacttagt
    23041 tacccccttt ctttttcttg aatcttatag gaaaaaaatc taagcctcca aaaggaacct
    23101 gaatctcctt ggaggcttcg tattggcatc ttggaagggg gcgaaatcga gaaagggaga
    23161 gccccgttat ttttctactt tgacccaatc agcggccaca ggcgggtggg taaagaactc
    23221 tatatagcga atttccttgt ctgaagtgtt ctcgatcgta tgttttaccc ctggaagaat
    23281 aaaaatcaca tcacccgctt taatgggaac tttcacgcca tcaacgatct cgttgcccgt
    23341 tccgctgata aaatacaaaa tggattcgcg atccttgtgg taatggtagg ctaattttgc
    23401 cccggtagag ggtggcggga gaatgacaaa aatcccattg aggtgtttgg ccttcatttt
    23461 ttcgtttacg atttcggtaa agtaaaattt atccttgttg ggattggcca ttttggcgaa
    23521 ctcttggcta tcaaacaatc tgaatccagg gagctccttt gtctgggcaa atgccccaga
    23581 tggaattaaa agaaccatga aaagaactcc gaccatccca accctaaaaa tactcgtgta
    23641 catattggcc tccctttttg atttttattg ctctattaaa tatggctatg gcaccccgac
    23701 ttgttgaaat tcttgcacac atccggtaac tgattgatta ccgattcgct gaaaatggag
    23761 gaaatttaat gggtgatggg gacggaaaaa gaagctggga aagagaatgt caaacggaag
    23821 ataatcggaa gagacatcca atcttcaaat cattttcgtt agttaaaatc aagttggcgc
    23881 ttgtggcttg gttttcattt caccttcgga gctcgtcttc tccaggaggg atttataaat
    23941 ggcctgggtt ttggctgagg gttcgatgcc aagggccgaa gacaggattt tccggcaccg
    24001 atggtacacc gcccttgctt ccgcctctcg atccaggcgt agatagcagg tcatgagatt
    24061 ctggtaaaat tcttccgcaa gatcgtccgc atccaaccct cgctggtagc attccaaggc
    24121 tttttcccat tggccggttt gttcccaatg ttgacctaac ctgtttacgc aacctaaaaa
    24181 cttgctcctc agccgttctc ggagggaaac catccagggt tgttcgggtt ctccgccaag
    24241 gaaggctcct cggtacaccc cgatcgcttt ttcagttaat tgaaaggagg cgtcaatccg
    24301 cccttcctgt ctctgggtgt ctgcctgccg ccagatattt tccaaagccc aaacatccaa
    24361 ccagcagtat ctcttatcaa gggtcagctt tccttccttg aattggaagg ccttttcgca
    24421 gccggctaat tggcggagac ggtggagggc agatatgaac gaacgatggg cggcatcgcc
    24481 atcggcctcc ggccaaagga tatccgccat catctcctct tttacctctc ggccccctaa
    24541 ggcaatcaat actttgagca tggaaagagg cttctgttga acttttctcg aaaactggat
    24601 cggtttcccg tctttccaca gttcaaaccg ccccaaggtg aggattttca ctggccaggg
    24661 ccagttttct aaatggtaaa aaggctcatc cagagtcaga tttcgcttgc gaatgatttc
    24721 ccgcacatat tccacttcaa tcctggcctc aagcgcccgg gcacagagca gagacatggc
    24781 cgaaggggaa tcgacatagg tttccgtaat tccttcttcc ttgccgatcg ccagagcgtc
    24841 gcggaggaat gaaagccctg aggcttcttt tccttgggcg aaggcaaacc gtgcctcagc
    24901 tatcagaata aaaaatttca gtaaaaagtt ctttgacgga tcacatgtgc gagaagcttg
    24961 cgcgagttga tccgcagctt ccttgtggtt cccaagttca tgcatcgcat gagccttggc
    25021 tacgtgagca aaagactgag tgagcagcga tcccacatgg gtaacgaatt ttatggctaa
    25081 atctgcatga agag
//
LOCUS       DASPRR010000140         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_351703, whole genome shotgun sequence.
ACCESSION   DASPRR010000140 DASPRR010000000
VERSION     DASPRR010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351703"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VEL68_05255"
     CDS             complement(<1..577)
                     /locus_tag="VEL68_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HXZ35407.1"
                     /translation="MRINTFYYIKNLITDIHVASVTPTSRFGVEKVLEKVPFPSSKLI
                     LEYGPGTGNFTTTLLKYMPENSKLIAIEKNPDFCYLLKKTIHDPRLTVFQDSAENVLD
                     ILKACNGAGDLKADCIISGIPFSLLSQTKKTKILKNTHSALKRGGKFLAYQAFFQFPV
                     ILKNPLEELFGGVQSQYYMFSLPPLLILETTK"
     gene            909..2576
                     /locus_tag="VEL68_05260"
     CDS             909..2576
                     /locus_tag="VEL68_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ35408.1"
                     /translation="MEKPWYQSYAKGIPHQLHYEPTPMPEALARTAREFPDKTALIFI
                     DSKVSYQQLNDMANRFAHALIDMGVKPGDKVAMLMPNMPQLVAATYGAWRAGAVVVMN
                     NPLYTDVELEYQFNNSESTFLVAIDLICPRMIALRPKTKIKNLVAAHIRDHLKFPKKQ
                     LLPIVAKDKHRNIPPTPNVHEWMDLLKKYPASDPKVPIDFNGLACLQYTGGTTGVSKG
                     VMLSHANLSKNVQQIGAWFPGFNKGEITHLGVLPIFHSFGLTCCMNICIWMGWTDVLI
                     PRPEPQAILEAIHKYRVNFFPAVPTMYVGVLNHPEASKYNLTSIKGCFSGAAPLPVEV
                     IRDFEAKTGSQICEGYGLSETSPVVTTNPYGGKTKVGSIGLPVSDTDIKIVDLVDGTK
                     EIPVGEAGEVLVKGPQVTGGYYKMPEETANAVKDGWLYTGDIGKMDEEGYFYIVDRKK
                     DMVIAGGYNIYPREIDEVLFEHPKVLEACAVGIPDPYRGETIKAFVVLKPGESLTEEE
                     VIKYCSEKLAKYKVPKKVEFMASLPKSGVGKVLRKELRAMEMAKGKK"
BASE COUNT          641 a          704 c          711 g          588 t
ORIGIN      
        1 ctttggtcgt ttccagaatg agcagcggtg gaagcgaaaa catgtagtat tgggattgta
       61 cccccccgaa gagttcttcc aggggattct tcaggattac gggaaactgg aaaaaggcct
      121 ggtaagccag aaattttccc cccctcttca gcgccgaatg agtgttcttc aggattttcg
      181 tcttttttgt ttgggagagc agggaaaagg ggattccaga gatgatacag tcggccttca
      241 ggtcgcccgc cccgttacag gctttgagga tgtcgagaac gttctcggca gaatcctgga
      301 agacggtcag ccgcggatcg tggatggttt ttttcaatag gtagcaaaaa tccgggtttt
      361 tctcgatagc gatcaatttt gaattttcgg gcatgtattt caagagggtc gtggtaaaat
      421 ttcccgtccc cggcccgtat tccaatatca gcttgctgct cgggaaaggg accttttcca
      481 aaaccttttc aaccccgaac cgggaagtgg gggttaccga tgccacatga atgtccgtaa
      541 tcaggttttt gatatagtag aacgtattga tcctcatggc tttttcctat cctgatggga
      601 agatttgaaa actctagcag aaccgcgaac ctgggatgcc ttcttccctt tcgcttccgt
      661 tcaagccgac ggctcccaga attttaatat taatccccac agatatcaat aaaattttca
      721 ctctcaggag aatttcttgg ataatcaaga cttcgctttt cgggggggtc cccgtccatt
      781 tttttcttga aaaaatcttt tcccttgttc tataaggatg ggggaggagc tggaaaatac
      841 cggggatcgg gcggtccccg ccgttttttg atcaaattca aaacgactac cccgtaagga
      901 ggaaacaaat ggaaaaaccg tggtaccaat cctacgccaa aggtattccc catcagttgc
      961 attatgaacc cacccccatg cccgaggccc tggcccgcac cgcccgggaa ttccccgata
     1021 agaccgccct catctttatt gactccaaag tctcttacca acagctgaat gatatggcca
     1081 atcgcttcgc ccatgcgctc atcgacatgg gggtcaagcc gggggataaa gtcgccatgc
     1141 tcatgcccaa catgccccag ctggtggcgg cgacctacgg cgcctggaga gccggggccg
     1201 tggtggtgat gaacaatccc ctgtatacgg atgtagagct ggagtatcag ttcaacaatt
     1261 ccgagtccac tttcctggtg gccatcgatc tcatttgccc gaggatgatc gccctccggc
     1321 cgaaaaccaa gatcaagaac ctggttgcag cccacatccg cgaccatctc aaattcccca
     1381 agaaacagct cctgcccatc gtggccaaag acaagcaccg caacattccc cccaccccca
     1441 acgtccatga gtggatggac ctcctgaaaa aatacccggc ctcagacccg aaggtcccca
     1501 tcgattttaa cgggttggcc tgcctgcagt acaccggtgg aacgaccggg gtcagcaaag
     1561 gcgtcatgct gtcccatgcg aacctttcca agaatgtgca gcagatcggg gcctggttcc
     1621 ccggtttcaa caaaggggaa attacccatc tgggagtcct ccccatcttt cattccttcg
     1681 ggttgacctg ctgcatgaac atctgcatat ggatgggatg gacggatgtg ctcattcccc
     1741 gtccagaacc gcaggccatc ctcgaggcca tccacaagta ccgggtcaat tttttcccgg
     1801 ccgttcccac catgtatgtg ggcgttttaa accatccgga ggcttccaag tacaacctga
     1861 cctccatcaa ggggtgtttc tccggggccg cccctctgcc cgtggaggtc atccgggact
     1921 ttgaggccaa gaccggttcc cagatctgcg aggggtacgg gctttccgag actagcccgg
     1981 tagtgaccac caacccctac ggggggaaga ccaaagtagg ctccatcggc cttccggttt
     2041 cggatacgga tatcaagatc gtggacctgg tggacggaac caaggagata cccgtgggcg
     2101 aggcagggga ggtcctggtc aaagggcccc aggtcaccgg cggatattac aagatgccgg
     2161 aggaaacggc caacgccgtc aaagacggct ggctctacac cggggacatc ggcaagatgg
     2221 acgaggaggg gtatttctac atcgtagacc ggaagaagga catggtgatt gccgggggat
     2281 acaacatcta tccgcgggag atcgacgaag tcctcttcga acatcccaag gttctcgaag
     2341 cgtgcgccgt gggaattccg gacccttatc ggggagaaac catcaaggcc tttgtggtcc
     2401 tgaagcccgg agagagccta accgaagagg aagtgatcaa gtactgcagc gagaaactgg
     2461 ccaagtacaa ggtccctaaa aaggtggaat tcatggcctc cctgcccaag agcggagtgg
     2521 ggaaggtcct gcggaaagaa ctgcgcgcga tggaaatggc taaggggaaa aaataagagt
     2581 gtcagcgttt agtgtcagtg tcagtaaggg caaacgggaa gggtagtggt ttttactgac
     2641 actg
//
LOCUS       DASPRR010000141         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_352506, whole genome shotgun sequence.
ACCESSION   DASPRR010000141 DASPRR010000000
VERSION     DASPRR010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352506"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /gene="panB"
                     /locus_tag="VEL68_05265"
     CDS             <1..669
                     /gene="panB"
                     /locus_tag="VEL68_05265"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXZ35409.1"
                     /translation="TMEEMIHHAKAVVRGAGSCLVVCDMPFMSYQTSVDEAIKNAGRL
                     MKETGVDCVKMEGGVEIKEKVRAIVEAGIPVMGHIGLLPQRVSLEGKYRVQGKTAASA
                     KEILEDALAIEKAGAFCIVLESVTAEGARMITERLKIPTLGAGSGPYLDGQSLNLYDI
                     LGLSLGIVPRFAKKYLNLGEDVVRALGEFKKEVDEGKFPAEENFFRMDPKEVQKLDAG
                     KKRS"
     gene            673..2205
                     /locus_tag="VEL68_05270"
     CDS             673..2205
                     /locus_tag="VEL68_05270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXZ35410.1"
                     /translation="MAGVEFSPLQPGPRGKDDHSRLPIILIAAGFGLFRLLYAGLLNL
                     SPQEAYYWVWSLHPALSYFDHPPMAAYSIWASTALLGDTVFAVRLPAILYGTGTTLVV
                     YSLAARLFGSRAGWVCALLMNLTIGFTAPFFFTTPDSPLIFFWSLTLLWVWKAAGEGR
                     KAFWYLAGIAWGLALLSKYTAAFLGVSTLLWLLLARDLRKEFKRPGLYSAVLLAAIVF
                     TPVILWNTQNHWASFLFQSKERFSHPVFISLENISTFFASQAGLMNPLLFLGFLAAFY
                     KGIRRWRKGAGQEEKFLLASPLVPLVFFALASTRVYIKVNWPTMAYPAVLILLVEYYR
                     RGVWPARWIRRVYVPALWVLAAVFCILVHLLIPWKAIPVSSSLDTVTGWPEAAAHVRG
                     LQKELSSQGSTFILAWDHKTAAELQFYLGRNEPVYCRNLIGLHALAYDFWEVPRELEG
                     KNTLLVWTNVDAITTEGEEKARRAFSSLSRLNAFDVYRGRQKIRTFYFSHGVDYHPPK
                     TL"
BASE COUNT          439 a          690 c          655 g          489 t
ORIGIN      
        1 accatggaag agatgatcca ccatgccaaa gccgtggtcc gcggggctgg gagttgcctg
       61 gtggtctgcg acatgccgtt catgtcctac cagaccagcg tggatgaggc gataaagaac
      121 gcaggccggc tcatgaagga gaccggcgtg gattgcgtca agatggaagg gggagtggaa
      181 atcaaggaaa aagtcagagc catcgtggag gcgggaatcc cggtcatggg ccacatcggc
      241 cttctccccc aaagggtctc cctcgaaggg aagtaccgcg tacagggcaa aaccgcggcg
      301 tcggccaaag aaatcctgga ggatgccttg gccatcgaaa aagcaggagc cttctgcatc
      361 gttttggaga gcgtaaccgc cgagggagcc aggatgatta ccgaaaggtt gaaaatcccc
      421 accttgggcg cggggtcagg cccctacctg gacggccagt ccctgaacct ctacgacatc
      481 ctgggcctct ccctgggaat cgtcccccgc ttcgccaaga agtatctcaa cctgggcgaa
      541 gacgtggtcc gggccctggg ggagtttaag aaagaggtcg atgaagggaa gttcccggcg
      601 gaggaaaatt tcttccgcat ggaccccaag gaggtgcaga agctggacgc cgggaagaaa
      661 agaagttgat tcatggctgg agtcgaattt tcaccccttc aacccggccc gcgtgggaag
      721 gatgaccatt cccgcttgcc gatcatcttg atcgccgccg ggtttgggct tttccgcctg
      781 ctctatgccg gcctcctgaa cctgagccct caggaagcct attattgggt ctggtccctc
      841 catccggctc tcagctattt cgaccaccct cccatggcgg cctattccat ctgggcctcc
      901 acggctctcc tgggggacac ggtcttcgct gtccgccttc cggccattct ctacgggacc
      961 gggacgaccc tggtcgtcta ttccctggcc gccaggctct tcgggtcgcg ggccggctgg
     1021 gtctgcgccc tgctgatgaa tctgaccatc gggttcacgg ctcccttctt tttcactacc
     1081 ccggacagcc ccctgatctt cttctggtcg ctcacacttc tctgggtttg gaaggccgcc
     1141 ggcgaaggca ggaaggcgtt ctggtatctg gccggcatcg cctggggcct ggcgctgctc
     1201 tccaaataca cggccgcatt cctgggcgtc tcgacccttc tctggctcct cctggcgaga
     1261 gacctccgca aggaattcaa aagaccggga ctctactccg cggtcctcct ggcggcgatc
     1321 gtcttcacgc cggtgatcct gtggaatacc cagaaccact gggcctcttt cctcttccaa
     1381 tcgaaggaac gcttctctca cccggttttt atctccctcg agaatatttc cacttttttt
     1441 gcctctcagg cggggctcat gaatccgctt ctcttcctgg ggttcctggc cgccttctac
     1501 aagggcattc gccgctggag aaaaggggcg gggcaggaag agaaattcct actagccagc
     1561 cccctcgttc ccctggtttt cttcgcgctg gcgtccaccc gggtgtatat caaggtgaac
     1621 tggccgacca tggcttatcc cgcggtcttg attttgctgg tggagtatta ccggcgggga
     1681 gtgtggcccg ctcgatggat tcgaagggtt tatgtgcccg ccctgtgggt cttggcggcg
     1741 gttttctgta tcctggtcca cctgctcatt ccctggaaag cgatccctgt ttccagcagc
     1801 ctggatacag tgacgggctg gcccgaagcg gccgcccacg tgcggggcct gcaaaaagaa
     1861 ttgtcctccc aggggtccac cttcatcctg gcctgggatc acaagaccgc cgccgagctg
     1921 cagttctacc ttggccggaa tgagccggtg tactgccgca acctgatcgg actgcatgcc
     1981 ctggcttatg atttctggga ggttccccgg gagttggaag ggaagaacac cctcctggtc
     2041 tggacgaacg tggatgcaat aaccaccgaa ggggaggaaa aagccagaag ggccttctct
     2101 tccctaagca gactgaacgc gttcgacgtc taccgcggtc gccagaagat ccgcacattc
     2161 tacttttccc acggcgtaga ttaccatccg cccaaaaccc tctaaatcct tcgggggagg
     2221 ctctcaatat gaaaagggac tgcaaaatct caaagaccca ttaagggcca atc
//
LOCUS       DASPRR010000142         4209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_190733, whole genome shotgun sequence.
ACCESSION   DASPRR010000142 DASPRR010000000
VERSION     DASPRR010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190733"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VEL68_05275"
     CDS             complement(<1..494)
                     /locus_tag="VEL68_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXZ35411.1"
                     /translation="MRSFKKPLIGLVLLFAVYSLFGFFALPPILKGILIKQLSENLHR
                     EVTIEKIQVNPYILSLTAQGFKVKERGSSEIFASGEEIYLNLQALSALYRELILKEIR
                     VRQPFLHLVRNPDESYNFSDLLEKKPSKTEEKGKPFLFSFNNIQIKDGSIDFEDKIPG
                     KKHTV"
     gene            798..2138
                     /locus_tag="VEL68_05280"
     CDS             798..2138
                     /locus_tag="VEL68_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ35412.1"
                     /translation="MEKGSSRAKKKGRSLKDRLGIFGPAILITILGFIVAYPFVEPAP
                     PRKIIIGTGDPSGAYYAFAQRYRELLAKDGITLEIRSTAGSVENVKLLEAREGGVDIA
                     FVQGGIGDPTAQPNLRSLGSLYFEPLWVFYQRGEKIKRLPDLRGKRIIVGPEESGVKA
                     IAVKLLEANGVNSQTATLLPLGGEKAVEALREKRADAAFFVLSPQSPLIRDLFSLKGV
                     DLLSFERGEAYQRANRFLSLLKLPEGVMDFEKNSPDRDTLLLAPAANLVVRQDFHPAL
                     ADLLIGVAMEVHGAPGLFQELNEFPSQKYLDFPLHKEAQRFFRTGPTFLKKYLPFWAA
                     VFIERAKIMFLPLFTLLLPLMRLFPPGFRWRMRSRIYHWYRNLEEMDFQGHQSTSPEE
                     VRSCIAELEKIDGEVMHVPVPLSYAGELYNLRIHIAHIQESLRKAEAVLRGEGK"
     gene            complement(2186..3337)
                     /gene="mutY"
                     /locus_tag="VEL68_05285"
     CDS             complement(2186..3337)
                     /gene="mutY"
                     /locus_tag="VEL68_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXZ35413.1"
                     /translation="MPHLRHAGFQVHQNFLGSLPALSRKAPPEKSVFQRSLLRWYETH
                     QRSLPWRTNANPYNILVSEFMLQQTRVETVVPYYQRFLALFPTLKDLAHAPLQKVLKA
                     WAGLGYYARARNLHDAAKVICRELGGKIPGTKEELLRLPGFGPYTAGAVASLAFNQPV
                     AALDGNVKRVIGRLFPEFTDPGRASAKGNLEKFLEEWIPPNRASDFNQALMDLGATVC
                     IPSKPRCSGCPVLKFCAAKGEPSGKKRVVRKFREETWAVALVEREGRYLLHRNEAKGL
                     LAGLWQFPKVVVEKNRGKEAGHKDPGKLLKKYLLEDFGLEVKMKSRLPEREYFFTHIH
                     ALMKPYLCYLSDPRNPSPLSKSVRWVKPSGFSRYPISTAMRKMAALLNR"
     gene            complement(3289..3795)
                     /locus_tag="VEL68_05290"
     CDS             complement(3289..3795)
                     /locus_tag="VEL68_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HXZ35414.1"
                     /translation="MALPGSQTEKNLKEAFAGESQANRRYLAFAKKAEEEGYKQVAKL
                     FRAAAEAETVHAHAHLRALAGVKSTKENIQEAIAGETHEFKKMYPEFLAKAKAEAIKP
                     AEMSFDYANQVEKIHAALYQKALESLGKNAAVDYYVCPVCGNTVEGKAPDECPICGTP
                     GSKFTKIS"
     gene            complement(3861..>4209)
                     /locus_tag="VEL68_05295"
     CDS             complement(3861..>4209)
                     /locus_tag="VEL68_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35415.1"
                     /translation="KKMRYLSIALLLLLALAVGCAPTIEGKKIDGAKTKDLLAEGTDG
                     SKVVQMFGEPQQKEQLPSGETKYIYYYKMDGKDQQRLEVFLKDNTVQRYRYVYNEEET
                     ITKDVPPIEPEKK"
BASE COUNT          910 a         1165 c         1128 g         1006 t
ORIGIN      
        1 acggtgtgtt ttttcccggg gatcttatcc tcaaaatcga tgctcccatc cttgatctgg
       61 atgttgttga aggaaaaaag gaaaggtttc cctttttcct cggtcttgga cggctttttc
      121 tccaggaggt cggagaaatt ataggactcg tctggatttc gaaccaagtg aaggaacggc
      181 tgccggaccc ggatctcctt gaggatcaat tcccgataga gggctgacaa ggcctggaga
      241 ttcaggtaga tctcctcgcc agaggcgaag atttcggagg atccccgctc tttgaccttg
      301 aacccctggg cggtcaggga gaggatgtag gggttcacct ggatcttttc gatggtcacc
      361 tcccggtgga ggttctccga aagctgtttg attaggatgc ccttcaggat gggaggcaag
      421 gcgaaaaacc caaagaggga atagaccgca aaaagaagga cgagaccgat caacggtttc
      481 ttgaaactgc gcatattagc ctcctttcat tgggttatcg atcctaactg tcacccgttg
      541 acagccgggc acccagaaaa accaataaac ctgtgcgggc gcgttggctc caaagggaga
      601 aaacatgaga gttagcataa tccaaaacat gatggaatcc tatgaaaaag ggaaacggtt
      661 cagctgcccc attaggtttt ttatttgcat atgctaaatt cattttggta aaatttcaac
      721 tccattcatt gttttcatcg ctcgccacgg gcagcccctg gggtcaaggg aaaaatggct
      781 tttgccagga ggtgggtatg gaaaaagggt ccagccgggc aaaaaagaaa ggacggagtc
      841 ttaaagaccg cctcggaata ttcgggccgg caattctgat caccattctg ggctttatcg
      901 tggcctatcc gttcgtggag cccgctcctc cccgaaaaat catcatcggt acgggcgacc
      961 cgtctggcgc gtactacgcc ttcgcccagc gctaccggga actcctggcc aaagatggca
     1021 ttacccttga aatccggagc acggccggct cggtggagaa tgtgaaactc ctggaggcca
     1081 gagagggagg ggtggatatc gccttcgtgc agggcgggat cggagacccc acagcccagc
     1141 caaaccttcg atctctcggc agcctctatt tcgagccgct ctgggtcttt taccaacggg
     1201 gagagaagat taaaaggctt cccgacctgc gggggaaaag aatcattgtc ggccccgaag
     1261 aaagcggcgt gaaggccatc gccgtaaaac tcctggaggc caacggagtc aactcccaaa
     1321 cggctacgct gctgccgctc gggggagaga aggccgtgga agccctgcgt gagaagcggg
     1381 ccgacgccgc cttttttgtc ctctcccccc aatcgcccct tatccgggat ctcttttcct
     1441 taaagggagt cgacctcctc agcttcgaaa gaggggaggc ttaccagcgg gccaaccgtt
     1501 tcctctccct tcttaagctc cccgaggggg tgatggactt cgagaagaat tctcccgacc
     1561 gggataccct cctcctcgcg cctgcggcca accttgtggt tcgccaggat ttccatccgg
     1621 ccttggcgga ccttttgatc ggagttgcca tggaggttca cggcgctccc ggattgttcc
     1681 aggaactgaa cgaatttccc tcgcaaaaat acctcgattt ccccctccat aaagaggccc
     1741 agcgcttttt ccggaccggc ccgacgtttc taaaaaaata cctccccttc tgggcggcgg
     1801 tattcatcga gcgagccaag attatgtttc tccccctgtt caccctgctc ctccccctca
     1861 tgagattatt tcccccgggc ttccgttgga gaatgcgatc ccgaatctac cattggtacc
     1921 ggaatctcga agagatggac ttccaggggc accagagcac ttccccggag gaggtccgtt
     1981 cctgtatcgc cgaacttgaa aagattgacg gcgaggtgat gcatgtgcct gtgcctctct
     2041 cctatgccgg ggagctttat aacctgcgaa tccatatcgc ccatattcaa gaatctctgc
     2101 gaaaagccga agcggttttg cgcggcgaag ggaaataaag ggctccccca atccgggaga
     2161 agggacctcc gctttctttt attacctacc ggttcagcag agcggccatt ttacgcatgg
     2221 ccgtggagat gggatagcgg gaaaaaccgg agggctttac ccagcggacc gacttggaga
     2281 ggggagacgg gtttcgcgga tcggaaaggt agcaaaggta aggtttcata agggcatgaa
     2341 tatgggtaaa gaagtactcc cgctcgggca gccggctttt catctttacc tctaaaccga
     2401 agtcttccag aagatatttc ttaagcagtt tccccgggtc cttgtggccc gcctcctttc
     2461 cccggttttt ctctaccacc accttgggga attgccagag tcctgccagg agacccttgg
     2521 cttcattccg atgaagcaga taccggcctt ctctttccac cagggcaacc gcccaggttt
     2581 cttcccggaa ttttctgacc acccgtttct tccccgaggg ctcacccttc gcggcgcaga
     2641 acttcaagac cgggcaacct gagcacctgg gcttggaagg gatgcagacc gtggccccca
     2701 ggtccatcag ggcttggttg aaatcagacg ctctgttggg ggggatccac tcttccaaga
     2761 atttttctaa gttacctttg gctgaagctc ggcccggatc ggtaaactcc ggaaagaggc
     2821 gtccgatgac gcgcttaacg tttccatcca gggcggccac cggttggttg aaggccaggc
     2881 tggctaccgc cccggcggtg taaggcccga agcccggaag ccggagaagt tcttccttcg
     2941 tcccggggat tttaccaccc aactcgcggc aaatcacctt tgccgcatcg tggaggttcc
     3001 gcgcccgcgc ataatagccc agtccggccc aggctttaag aaccttctgc agcggggcgt
     3061 gagccaggtc tttcaaggtg ggaaaaaggg ccaggaagcg ctggtaatag gggacgaccg
     3121 tctccacccg ggtctgctgg agcatgaact ccgagaccag gatattataa gggttggcat
     3181 ttgtgcgcca ggggaggctt cgttgatggg tctcatacca ccggagaaga gaccgctgga
     3241 aaacggactt ctccggtggc gcttttctgg ataaagcagg caaggatcct aagaaatttt
     3301 ggtgaacttg gaacccggcg tgccgcagat ggggcactca tccggggctt ttccttccac
     3361 cgtgttgccg catacggggc agacgtaata atccactgcc gcatttttcc ccaggctttc
     3421 caaggccttc tggtagaggg ccgcgtggat cttttccacc tgattggcat agtcgaagct
     3481 catctccgcc ggttttatgg cttccgcttt cgccttggcg aggaactcag ggtacatttt
     3541 tttgaactca tgggtttccc cggcaatcgc ctcctggatg ttttccttgg tggacttcac
     3601 cccagccaaa gcccgaagat gagcgtgggc atgaacggtt tccgcctcgg ctgcagcccg
     3661 gaagagcttg gccacctgct tgtacccctc ttcctcagcc ttcttggcaa aggcgagata
     3721 cctgcggttc gcctgagatt cgcccgcaaa agcctctttc aaattctttt cggtctgtga
     3781 gcccggtagc gccattttgc aagacctcct cgataaattc tcgctaaggg ttttaagaaa
     3841 tttgcccctt caatgcttct ttatttcttc tcgggttcga tgggaggaac gtccttggtt
     3901 atggtttctt cttcattgta gacataccga tatctctgta ccgtattgtc tttaagaaag
     3961 acttccagcc tctgctggtc cttgccatcc atcttgtaat aataaatgta tttggtttca
     4021 ccggagggca gctgctcctt ctgttggggt tcgccgaaca tctggacaac tttgctccca
     4081 tccgttcctt cggccaacaa atccttggtc ttggcgccgt cgatcttttt gccctctata
     4141 gttggagcac aacccacggc caatgctaag agaagaagca aagcaatact cagatacctc
     4201 attttttta
//
LOCUS       DASPRR010000143         8286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1972956, whole genome shotgun sequence.
ACCESSION   DASPRR010000143 DASPRR010000000
VERSION     DASPRR010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972956"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VEL68_05300"
     CDS             complement(<1..1358)
                     /locus_tag="VEL68_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HXZ35416.1"
                     /translation="MVLLLTWVPISVFAQPAAKGESSYSPVVIKETFQAIMDRMKAAK
                     PGVMKRQMDLLSERYDLSNRPAQGVTMSRGKAVQEGVRAKLPQGVTWAQLAAMNPEEV
                     REKNLFPAGFLPLPHPNHPEGGMLFPKFHIDEVKKQEGRDLTRFDLDYDLPDHFLPEF
                     PAPIYLTTRPDLGDVSKGKLVTIDNYYELFNGILNPKQIEGLRLLLTPFPQQQFNQTE
                     DRRSERPSRGVVCFDCHANGHTNASTHLVGDIRPQEFRHRLDTPSLRGVNIQRLFGSQ
                     RALKSVEDFTEFEQRAAYFDGDPVIATKKGVNILERGSQVHFMAEFQELLDFPPAPKL
                     GITGKLDPKKASETEKRGQDLFFGKAACGICHPAPYYTDNLMHNLKTERFYQPKMVNG
                     RYAAADGPIKTFPLRGIKDSPPYLHDGRLLTLEDTVEFFNIVLELKLNEQEKKDLVAF
                     MRA"
     gene            1828..2424
                     /locus_tag="VEL68_05305"
     CDS             1828..2424
                     /locus_tag="VEL68_05305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ35417.1"
                     /translation="MVKKDFFNQMAANWDGRFYTTEMAERLPKLVSSFRVQKGAKILD
                     VGAGTGGIIPYLLEAIGQGGSLWAIDYAEEMVRIGRTKFQKESRVVFELAPVETLPYK
                     DQFFDQVICFGAFPHFEDKERALEEMGRVLKSGGTLIIAHALSSVALRSHHQNCGPVS
                     KDFLPEEGEMRSLLEKAGFQLKRLIDQPNCYLCEGEKA"
     gene            2587..3222
                     /locus_tag="VEL68_05310"
     CDS             2587..3222
                     /locus_tag="VEL68_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXZ35418.1"
                     /translation="MYTLIRQGITSGRFVPGQRLSERELAGKYGVSRTPIREAFRHLI
                     QEGLVVYKPNSGYRIIPLSEELAQHILVVRETLESIAARLAALRDPKGTAEAMAKTLP
                     RARCAYREGQLSELITANQGFHQALVENSGNPVLSAMYHTLQGYIGLMMSFSLSWPRR
                     PLETIWEHGEIIRALKSGDPDAAEKAVRRHVRSAYAGVLRNVRQYLSQTVR"
     gene            3298..4839
                     /locus_tag="VEL68_05315"
     CDS             3298..4839
                     /locus_tag="VEL68_05315"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013880200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXZ35419.1"
                     /translation="MKEGIKKLEELKQKASLGGGKERGEKLRQEGYLTAEERINLLLD
                     PGTFVEFGTLAGPHPAEFGPAPAYIPRDGVITGFGQVNGRTVAVYAHDKTVQGGSLGN
                     VQATKTSSLIRRACELKVPVVALQDSVGARIQEGNKNMGFDSIFFALTQASGVVPLIS
                     AILGRCAGGIAYSSALMDFILMVQGKTQLFITGPEVIKAVTHEEVSFEALGGAGVHSE
                     KSGVCDLVAPDEGAAIAMIKELLSILPGSYLEQPPRFPTRDCPEGSMEKIREIIPADP
                     KKFYDMKKILRFVLDEGYFFEIKAKFARNIFTGFGRVDGRPVGIIANQPMHLAGALDV
                     DASDKASRFIRFCDAFNLPVLTFVDIPGYLPGVDQEHRGIIRHGAKMLFAYAEATVPK
                     ITVIVRKDYGGGKQAMCSKPMGTDLVLAWPSAELAVMGAEGAVNVVCRKEIQESKDPE
                     GVRKRLVGEYEKIFGGPFESARKMYVDDVIRPEETRLKIIQALRIFQNKKAGRMNKKH
                     GNMPV"
     gene            5017..6207
                     /locus_tag="VEL68_05320"
     CDS             5017..6207
                     /locus_tag="VEL68_05320"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ35420.1"
                     /translation="MSQPLKGIRVLDLTHVLAGPFCTQILGDLGAEVIKIERPGVGDP
                     TRRMPPHTMKDQSLYFMSLNRNKKSLTLDLKAPEGKEIFYGLTRKADVVMNNFTPGTM
                     EKLGLGYEDLKKINPKIVWASVTGYGQTGPYRERPSYDIIVQAMGGAMSYTGEPGKPP
                     VRCGIPIGDLGGAVFAVIGILSALVSRSMTGEGRMVDISMLDVQLALHTYRAKYYFVA
                     GEVPQPVGTAHVSNVPLRAYRTKDSALVIEAYIDHFFRNLCKNLDMEHLADDPRFSTR
                     ANRLKNREELDRILEEAFLTKTTAEWWDLFIKLEVPSGPIYTVAEAVADPQVLSRNMV
                     VEIDSPHVGKTRDVGSPIKVSGVDQTQYVYPPALGEHTEQILKNLLGYSADKIKGLKS
                     QKVV"
     gene            6354..7811
                     /locus_tag="VEL68_05325"
     CDS             6354..7811
                     /locus_tag="VEL68_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ35421.1"
                     /translation="MALKTRAEYLASLKKLRPNIHKFGERIEDVTTHPATRRVVESHA
                     RAYDAAQEPALSGLFTTTSTLTEEKVHRHNSLMMSMEEVIYNSKFKREMYRLTGTCTG
                     ALCAGWNGMNTMYAVTHEMDAALKTDYHLRIKKYALQAQADGWTIAGALTDAKGDRSL
                     KASQQPDPDSNLRIVEVQEKGIVLRGAKCMICGVAASNEIFLLPGTAYGESDKNFALS
                     CAVPRDIEGLTIIETRRPSDTREQEEGFDIPTETGITQAYLLFEDVFVPRERVFLCKE
                     FAFTGKVIQYFTSIYRACIGACVAGQGDVMIGAGVLMARANGLSAKTFASKFIEMAVN
                     NETTFTMGVGSIALGGRHPSGVWIPDSLTSHTNKIHVATLPYETKRLCQEIGGGIVET
                     GCFPSYRDFTDSKYGKLVQKYVKAGSCSAEARARTARLNEWLTLGAGVPGCMHGGGSP
                     DGARLVVRSLLPLEKYAQMARKLGGIKEEIPDPGK"
     gene            7944..8072
                     /locus_tag="VEL68_05330"
     CDS             7944..8072
                     /locus_tag="VEL68_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HXZ35422.1"
                     /translation="MPNKVGQVYVCEICGNKVEMVVVGKGTLHCCGQPMTLAGEKK"
BASE COUNT         1963 a         2194 c         2402 g         1727 t
ORIGIN      
        1 gccctcataa aggccacaag gtcttttttc tcctgctcgt tgagcttgag ttcgaggacg
       61 atattgaaga actccaccgt atcctccagg gtcaacagcc gcccatcatg aaggtagggt
      121 ggagaatcct ttataccccg gaggggaaaa gtcttgatcg ggccatcggc ggcggcatac
      181 cggccattga ccattttggg ctggtaaaag cgctcggtct tcagattgtg catcaggttg
      241 tcggtgtagt atggcgcggg atgacagatg ccacacgcgg ccttgccgaa gaaaaggtct
      301 tggcctcgct tttccgtctc ggaagctttt ttcgggtcca gcttccccgt aatcccgagc
      361 ttgggagccg ggggaaaatc aaggagctcc tggaattccg ccatgaaatg gacctggctt
      421 ccccgttcga gtatattaac gccttttttg gtggcgatta ccgggtcccc atcgaagtag
      481 gccgcgcgct gctcgaattc cgtgaagtct tcaacggatt tcaaggcccg ctgggagccg
      541 aagagccgct ggatattcac tccgcgaaga gacggggtat cgaggcggtg ccggaattcc
      601 tgcgggcgga tgtcccccac cagatgggtg gaagcgtttg tgtgcccgtt ggcgtggcag
      661 tcgaagcaga ccacgccgcg gctaggccgt tcggagcggc ggtcttcggt ctggttgaac
      721 tgctgctgcg ggaagggggt cagcagcaaa cgcagccctt ctatttgttt tgggtttaga
      781 attccgttga agagctcata ataattatcg atagtcacca gcttcccctt ggagacgtca
      841 cccaggtccg ggcgagttgt cagataaata ggcgccggaa attcagggag gaaatggtcc
      901 ggcaggtcat aatcgaggtc aaatcgggtg aggtcccgcc cttcctgctt cttgacctcg
      961 tcaatatgga acttgggaaa aagcattccc ccctcggggt ggttagggtg agggagaggc
     1021 aggaatcctg cgggaaagag attcttctcc cggacctcct cggggttcat cgccgccagt
     1081 tgcgcccagg ttaccccctg agggagtttg gcccgaacgc cttcctggac ggcctttccc
     1141 cgggacatgg taaccccctg ggccgggcgg ttgctcaggt cgtagcgctc gctgagcagg
     1201 tccatttgcc tcttcatcac ccccggcttg gccgccttca tgcggtccat aatggcctgg
     1261 aaagtttcct taatcaccac cggagagtag ctggattctc cctttgccgc gggttgggca
     1321 aagaccgaga tcggcaccca ggtcaatagg agaaccatta gcatcaagac accgtacttt
     1381 gctgtttttg acatgtggac aaacctcctt tccctctttt gggttttagc gccagagagg
     1441 gccgattgac tttgcatgca gttgccctca aaatctctct gcggctcagg gcaggcggtt
     1501 atgagcatgg gagaaacagc tctctttttt tcccctgaaa taataaaatg ccgctgcaca
     1561 gctgggaagt aaatagggtc aacaccctag tttgagacga tattcactgt attttcaaga
     1621 tcccccttgg ccaagcttcg aggaatggag gttggaggga agcaggaggg gaaggggggc
     1681 ttggctatga atggtccgag gcaatttgga aaagaatttc tgagacctcg ggttggtcga
     1741 gaccctttcc ttccctcttc gaagtccttg agctctggtt tgaacgctag tagaatttca
     1801 aatgaagccg gtaacaggaa ggatggaatg gtcaagaaag atttctttaa ccaaatggca
     1861 gccaattggg atggccggtt ttataccacc gaaatggcgg agcggcttcc caagctggtt
     1921 tcttcgttcc gggtccaaaa aggagccaaa attcttgatg tgggggctgg aaccggcggc
     1981 attatccctt atctcctgga agccattggc cagggaggat ccctctgggc gatcgattac
     2041 gccgaagaga tggtcaggat cgggcggacg aagtttcaga aagaaagtcg agtcgttttc
     2101 gaactggctc cagtggaaac cctcccctac aaggatcaat tcttcgatca ggtgatctgc
     2161 ttcggcgcat tcccccattt tgaagacaag gagagggcct tggaagagat gggacgagtc
     2221 ctgaagtccg ggggaacgct gatcatcgcc cacgctctga gcagcgtagc ccttcgcagc
     2281 caccaccaga actgcggccc ggtgtccaag gatttcctgc ccgaggaagg agaaatgaga
     2341 tccctcctgg aaaaagctgg gtttcaactg aaacgtttga tcgatcagcc caattgctat
     2401 ctctgcgaag gggaaaaggc ttaaccttcc tcctccggga tttttgtgac tccgtcccaa
     2461 cctgtacccc caaccccccc ataaatggaa aaaggtttga cagacctagt taaaatggta
     2521 taccattttg gtatggaatc agtcccggaa cccagaaagc aagaaaggaa aattaaggga
     2581 ccggatgtgt acactctgat ccgccagggt atcaccagcg ggcgcttcgt tcccgggcag
     2641 cgcctaagtg aacgggaact cgccgggaaa tatggcgtca gccgaacccc cattcgggag
     2701 gccttccgcc acctgatcca ggaagggctg gtcgtctata aaccgaactc gggctaccgc
     2761 atcattcctc tctcggagga attggctcag cacatccttg tggtgcggga aaccctggag
     2821 agcattgccg cccggctggc cgccctgcgg gaccccaagg gtacggcgga agccatggcg
     2881 aagacccttc ccagggcccg ttgcgcctat cgggaagggc agttgtctga attgatcact
     2941 gccaatcagg gatttcacca ggctctggta gaaaacagtg ggaaccccgt cctttccgcc
     3001 atgtatcaca ctctacaggg gtacatcggg ctaatgatga gtttttccct ctcctggcct
     3061 aggcgtccat tggaaacgat ctgggagcac ggggagatca tccgggctct gaagagcgga
     3121 gaccccgatg ctgcagagaa ggccgtccgg cgacacgttc gcagtgccta tgcgggtgtt
     3181 ctcaggaatg tgagacaata cctgagccag acggtgagat gatgatggaa gatcaaagca
     3241 tttgcatgcg gaattgggaa tcatagaaga aaaaatggtt aggagtttgc catggagatg
     3301 aaagaaggaa tcaaaaagct ggaagagttg aaacaaaaag cgtctctggg cggcgggaaa
     3361 gagcggggcg aaaaactccg tcaggaagga tacctaacag ccgaggagcg aatcaacctt
     3421 ctcctcgacc cggggacctt cgtggagttc ggcactctgg ccggtccgca cccggcagag
     3481 ttcggcccgg ccccggccta catcccccgg gacggggtca tcaccgggtt cggccaggta
     3541 aatggccgga cggtggcggt gtatgcccat gacaagaccg tccagggagg atcgctgggg
     3601 aatgtccagg cgaccaagac ctcgagtctc atccggcggg cctgtgaact gaaggttccg
     3661 gtcgtcgccc tgcaggattc cgtaggcgcc cggattcagg aaggaaacaa gaatatgggg
     3721 ttcgacagta tctttttcgc cctcacccag gcctccgggg tagttccctt gatttcagcc
     3781 atcctcggtc ggtgtgccgg tggaattgct tacagctcgg ccctgatgga cttcatcctc
     3841 atggttcagg ggaagaccca gctcttcatc accggtccgg aagtgatcaa ggccgtgacc
     3901 catgaagagg tttccttcga agccttgggc ggggccgggg ttcactcgga gaaaagcggg
     3961 gtctgcgatt tggtggcccc cgatgaaggc gcggccatcg ccatgatcaa ggagctatta
     4021 tcgatcctgc ccggtagcta tctggaacag cccccccgct ttccgacgcg agattgcccg
     4081 gaaggatcga tggaaaaaat ccgggagatt attccggccg accccaaaaa attctacgat
     4141 atgaagaaaa tcctgcgctt tgttctggat gagggctatt ttttcgagat aaaagctaag
     4201 tttgctagga acatcttcac tggattcggc cgggtggacg gccggccggt ggggatcatc
     4261 gccaaccagc ccatgcactt agctggagcc ctggatgtgg atgcctccga caaagcctcc
     4321 cggttcatcc gtttctgcga cgccttcaac ctccccgtcc tgactttcgt ggacataccg
     4381 ggatacctcc ccggggtgga tcaggagcac cggggcatta ttcgccatgg ggccaagatg
     4441 ctcttcgcct atgccgaggc caccgttccc aagatcaccg tcatcgtgcg caaggactac
     4501 ggcgggggaa aacaggccat gtgcagcaag cctatgggca cggacttggt actggcttgg
     4561 ccgagcgcgg agctggcggt tatgggggcc gaaggggcgg tgaacgtggt ctgccgtaag
     4621 gaaatccagg agtcgaaaga cccggagggg gtccgcaaga gattggtagg agagtatgaa
     4681 aaaatctttg gcgggccctt tgaatccgcc cgcaagatgt acgtggacga cgtcatcagg
     4741 ccggaggaga cccgactcaa gatcatccaa gccctgcgaa tcttccaaaa caagaaagcc
     4801 ggccggatga acaagaagca cggcaatatg ccggtctaat agaattgcgg aacaaaaaat
     4861 tatgggacgc agaagaacgc cgatccttgc ggattttaga aaacctttaa attgggtttc
     4921 ggctttttga aaggtcttaa cggtgttaat gcgtcttcaa atgggtgtca ttaaaggggc
     4981 ttttctgtgt tccaatcgat taaaaaggag agaagaatga gccaaccttt gaaaggaatc
     5041 agagttctgg atttgaccca tgtgctggcc ggccctttct gcacccaaat cctgggtgat
     5101 ctgggtgcgg aggtcatcaa gatcgagcgt cccggggtgg gggacccgac gcggaggatg
     5161 ccgccccata cgatgaagga ccagagcctc tacttcatgt ccttgaaccg aaacaagaaa
     5221 agcctgaccc tggatttgaa agccccggag ggaaaagaga tcttctatgg gttaacccgc
     5281 aaggctgatg tggtgatgaa taacttcact cccggaacta tggagaaact gggactgggg
     5341 tacgaggacc tgaagaagat caatcccaag atcgtctggg cttcggtcac cgggtacggc
     5401 caaaccggcc cctaccggga gaggccctct tacgacatca tcgtccaggc catggggggg
     5461 gcgatgagct ataccgggga accgggcaaa ccacccgtcc gctgtggtat tcccatcgga
     5521 gacctggggg gtgcggtctt tgcggtgatc ggaatccttt ccgccctggt ctcccgttcc
     5581 atgaccggag aaggccggat ggtggacatc tccatgcttg acgtgcagct tgctttgcat
     5641 acctatcggg ccaagtacta tttcgtcgcc ggcgaagtcc cccagccggt gggcacggcc
     5701 cacgtaagta acgttcccct tcgggcctac cgcaccaagg attcggcttt ggtcatagaa
     5761 gcgtatattg accacttttt ccgcaatctc tgcaagaacc tggatatgga gcatctggct
     5821 gatgacccgc gcttcagcac gcgggcaaac cgtttgaaaa accgggagga gctggatcgc
     5881 atcctggaag aggccttctt gacgaagacc actgccgagt ggtgggacct cttcatcaag
     5941 ctggaagtcc cctccggccc catctacacg gttgccgagg cggtggccga cccccaagtc
     6001 ctttcccgca acatggtggt ggagatcgat tctccccacg taggcaaaac cagggacgta
     6061 gggagcccga tcaaagtctc aggggtggac cagacccaat atgtgtatcc ccccgctctg
     6121 ggagagcata ccgagcagat tttgaaaaat ctgctgggtt attcggcgga caagatcaaa
     6181 ggactgaaga gccaaaaagt ggtttgaatc tattttttga aggagccgcc tacaacgcgc
     6241 aaggacgact gcggaaacga tcatctgcag agtcaagaca aaacaagcct cgctttacca
     6301 taagccgaaa gggatgatag aataatccaa atctgaacga aggggggacc cccatggcct
     6361 tgaagacccg cgccgaatac ctggcgtcct tgaagaagct ccgacccaac atccataaat
     6421 tcggggaaag gatcgaagac gtaaccaccc atccggccac ccgccgggtg gtggagagcc
     6481 atgcccgagc ctatgacgct gcccaggagc ctgctctttc cggcctcttt acgacgactt
     6541 ccactctgac tgaagaaaaa gtccatcgcc acaactctct catgatgtcc atggaagagg
     6601 tgatctacaa ctccaagttc aaacgggaga tgtaccgcct caccgggacc tgcaccggcg
     6661 ctctctgcgc gggctggaac ggcatgaaca ccatgtatgc cgttacccat gaaatggacg
     6721 ccgccctgaa gacggactat cacttgcgga tcaagaaata tgcgcttcag gcgcaggctg
     6781 acggttggac tattgccggc gctttgactg acgcgaaagg cgaccgcagc ctgaaagcct
     6841 cccagcagcc cgatccggat tccaacctgc gcatcgtgga agtgcaggag aagggaatcg
     6901 tcctgcgtgg ggccaagtgc atgatctgcg gggtagccgc atccaacgag attttcctcc
     6961 ttcccggaac agcctacggg gagtcggata agaactttgc cctgtcttgc gcagttccgc
     7021 gggatatcga aggattaacc attatagaaa cacggcggcc cagcgacacc cgggagcaag
     7081 aagagggttt cgacattccc accgaaaccg ggattactca ggcctatctg ctcttcgaag
     7141 atgtctttgt accccgggaa cgtgtatttc tctgcaagga atttgctttc acagggaagg
     7201 taatccagta ttttacgtcc atttatcgcg cgtgcatcgg cgcatgcgtg gctggccaag
     7261 gagacgtcat gatcggggcc ggggtcttga tggccagagc caacggcctt tccgcgaaga
     7321 ccttcgcttc caagttcatc gagatggcgg ttaacaacga aactactttc accatggggg
     7381 ttgggtccat cgcgctggga ggccggcatc cttcgggggt ctggattccc gactctctca
     7441 cttcccacac caataaaatc catgtggcca ccctgccata cgaaaccaag cggctctgcc
     7501 aggagattgg gggtggaatt gttgagaccg gctgttttcc atcctaccgc gattttacgg
     7561 actcgaaata tggaaagctc gtgcaaaaat atgtgaaagc cggctcttgc agcgccgaag
     7621 cccgagcacg gacggcgagg ctgaatgaat ggcttaccct gggggccgga gtcccgggct
     7681 gcatgcacgg cggcgggtct ccggacggtg cccgtctggt tgtccgctct ctcctccccc
     7741 ttgagaaata cgcccagatg gccagaaagc ttggcggaat taaagaagaa atccccgacc
     7801 ccggaaaatg aaaaaataat gggaggcaga tgaactcaga aaaatcgggt taatagactg
     7861 taaagcttga taattttcaa aggattctac ctgcattgat cgccgaaaac cggcggccgg
     7921 ataaatgaaa ggagggcatt aaaatgccta acaaagtcgg gcaggtctac gtctgtgaaa
     7981 tctgcgggaa taaggtggag atggtggtcg tcggaaaggg aacccttcat tgctgcggcc
     8041 agcccatgac gctggcagga gagaaaaaat aggcgcccaa accaaaaacc ttgacttttg
     8101 tccaaaaatc cattaaaaaa aaacaactta ttgtttcggc gaggaagggc agactctccc
     8161 aagaaaggag aaaggggcta tggacgggta tgcgggaaaa attctgcggg tcaacctcac
     8221 aaccagaagg tgcaccgaag aacccctgaa tccaaagtac gccaaagatt acatcggcgg
     8281 aaccgg
//
LOCUS       DASPRR010000144         5665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1325404, whole genome shotgun sequence.
ACCESSION   DASPRR010000144 DASPRR010000000
VERSION     DASPRR010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325404"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VEL68_05335"
     CDS             <1..765
                     /locus_tag="VEL68_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ35423.1"
                     /translation="SKGVVYAKQNNIDLALEQFSKALKANPVNAEAYKRMAHTYGEMG
                     NLEKELQCYKKAVEVQEKNPLYAFHLGDVLMKKYGDFRQAHFYLQKAYRLNPDNYQYA
                     FHYANCSQILGRFEDALEVYDQMIQTSPKAPDGYMLKGHCLLKMKKYGQAIQSYSQAQ
                     TIRPLDFSAARNLALAHAELKDFESAIVHNEYALRIRPQDVDTLYLLQSLYRRQGRFE
                     EAYTAVREILKIQPGHAGAQRVLSYLEANRKKRVSS"
     gene            762..1808
                     /locus_tag="VEL68_05340"
     CDS             762..1808
                     /locus_tag="VEL68_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ35424.1"
                     /translation="MSPRVPNPPKTQSPILGYVRTSGVLFSLFLWANHIFLNYRVAGN
                     ILLAFLPIMGSITMYLWVILVADEFLLLESSERISRILRILKHLCFGVAIFYGALALG
                     LWVNGMNHSPTVSKRTKIVSLSDVDIGLQSYRHLTITGWDADHNPRTILSNDKDDLGL
                     HAGGDIEILLSEGALSLHRIIAIKQDKERFHLKMLTADPDSKVALAGLVEIYSRGKDF
                     GKALELFDQLYDKYPTEADIGQDLAARLIDSRNYEQAAGVLKKLVETKKDYELQILLG
                     QALIRAGKRDEAEKYLEEAQQLDPTDIRALFLLGYIYRDTGRKEEAKEAWTRVLELYP
                     NFAQAEKNIESLQK"
     gene            2206..3810
                     /locus_tag="VEL68_05345"
     CDS             2206..3810
                     /locus_tag="VEL68_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXZ35425.1"
                     /translation="MQEALGKYQMAEAVREAYGSGEDDETLEPRVLIDARGNPIGCFR
                     DGDYIIFYNLRGEREVELCQSLLDKEFSHFPVERNLRLNMVTMIPYHQKLKARVAFSP
                     EEEIRDTLSEILSRHGLRQVKVSESEKAIHVSFFLNGKNRKPFPGEERIAVPTPKGVA
                     QFDQKPEMNACGVADSVIQSLQDRQVSFWFANFANIDVLGHIENEAAIRKAVEAVDNN
                     LGRCLKAARQAGVVTMVTADHGTAEKWLYPDGTIDTGHTDSPVPFILVPPRSEGAFSW
                     SLRSSGGLTDVAPTVLEVLDIPKPPVMTGQSLLLKKPMKKGSGENRVLLILLDGWGYR
                     SETHGNLIAKARTPVMDDLQQGYPFTTLEASGEAVGLPRGTVGNSEAGHLHIGAGRRI
                     FSDRLVIDRAIQDGTFFHNDAFLWAIGGARKEGKNFHLMGIVSFFSSHGSMDHLMALF
                     KMAKQESVENLYHHAMLGRRGERKESGARYLETIEKELENLHLGRIVSVIGRYWSLDR
                     EENWDRIEKTYRMLVYGEGQKVRLRP"
     gene            3927..5249
                     /locus_tag="VEL68_05350"
     CDS             3927..5249
                     /locus_tag="VEL68_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXZ35426.1"
                     /translation="MKLFRILVVFTLVLGLVYAATAQAQQFNWQQFKGTQIRVLLNKH
                     PFQAGIEPSLKDFEQLTGIKLITEVYPEDQFRAKVLVELTSGASSIDVFMSMPAQEGL
                     KYIRAGWLQPVDEFLKNASLTAPDYNWNDFLDKTRDAMTIEGKLVGPPIQVENAALMY
                     RKDVFSKYGVKVPTTLDELEAAAKALNGKAMTDDGQPGFGITLRGKRAAATSQFAGML
                     HAMGSTWLTPSREPAFTSDEGIKAFDMWGRLLRLYGPPGSENNHWYEASSIFSQGKAA
                     MFVDANSLFPVIEDPQKSKVIGKVGYALYPRGPKGQYGSTVAVWGLAMPKSSKNQKAA
                     WLFMQWATAKAQVMNLQSEKGILGCRESVWKDPKGKGKTPADLAESLMQGSKMGTPLW
                     NPPVVAVAEVRDAVGAAIVTSIQGGDVKAAVNKAAADMKRIMAETEKK"
     gene            5320..>5665
                     /locus_tag="VEL68_05355"
     CDS             5320..>5665
                     /locus_tag="VEL68_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35427.1"
                     /translation="MTQEIQTAPGPSSVGEEKPNSWWSHLAEKHPVLLFTLPAGIVVF
                     VLMVFPVFYTLYMSLHSWFASSVTAPEFIGLQNFKRAFIQDERFRNSILLTLYFTILA
                     TAMQLVLGVALAL"
BASE COUNT         1361 a         1527 c         1596 g         1181 t
ORIGIN      
        1 tctaaagggg tggtctatgc caagcagaac aatatcgatc tggctttgga gcaattttct
       61 aaagccttga aggcaaaccc ggtgaacgcc gaagcctaca aaaggatggc ccatacttat
      121 ggggaaatgg ggaatctgga aaaggagctt caatgctaca agaaagcagt ggaagtacag
      181 gaaaagaacc ccctttacgc cttccacctg ggggatgttt taatgaaaaa atacggcgac
      241 ttcaggcagg cccatttcta cctgcaaaag gcctatcgat tgaatcctga taattaccag
      301 tatgctttcc attacgccaa ttgctcgcaa attctcggcc ggtttgaaga cgctctcgag
      361 gtttatgacc aaatgatcca gaccagcccc aaagccccag acggatatat gctgaagggc
      421 cactgcctgc tcaaaatgaa gaaatatggg caggcgatcc agtcctattc gcaggctcag
      481 accatccggc ccttggactt ttcggccgcg cggaatctgg cgctggctca cgcagagctg
      541 aaagattttg aatccgcgat tgtccataat gaatacgccc taagaattcg gccccaggac
      601 gtggacaccc tgtaccttct ccagtctttg taccgccgac agggaagatt tgaagaagcc
      661 tacacggcgg taagggaaat tcttaaaatt caacccggcc atgctggcgc tcagagggtt
      721 ctatcctatt tggaggccaa ccggaaaaaa agggtatctt catgagtcca cgagtcccaa
      781 acccccccaa aacccaaagc ccaatcctcg gctatgtccg gacttccggt gtcctctttt
      841 ccctgtttct ctgggcgaat catattttcc tgaattaccg ggttgccgga aacatcctct
      901 tggctttctt gcccatcatg ggctcgatca ccatgtatct ctgggtcatc ctggtggccg
      961 atgaattcct cttgctggaa agctcggaac gaatttcccg aatcctcagg attttgaagc
     1021 atctctgctt cggggtggcc attttttatg gggccctggc tctgggcctt tgggtgaatg
     1081 gaatgaatca ttcccccacc gtcagcaaaa ggacaaagat tgtttctctg tcggacgtcg
     1141 atattggctt gcagagttat cgccatctaa cgattacggg ctgggatgcc gaccacaacc
     1201 cgcgaacgat cctttcgaac gataaagatg atctgggact ccatgccggt ggggatattg
     1261 aaattctatt gagcgagggg gccctctctt tacatcggat aatcgccatc aaacaagata
     1321 aagaaagatt ccacctgaaa atgcttaccg cggacccgga ttccaaggtt gccttagcgg
     1381 ggttagtgga gatatattcc agagggaaag actttgggaa agccctggaa ttgtttgatc
     1441 aattatatga caagtatccc acagaagccg atatcgggca ggacctggct gcccgcctga
     1501 tcgatagtcg aaattacgaa caggccgcag gggttctaaa gaagctggtg gagacaaaga
     1561 aggattatga actacagatt ctgctggggc aggcactgat tcgggccggg aaaagggatg
     1621 aggccgaaaa gtacctggaa gaagcccagc aactggaccc gacggatatc cgggccctct
     1681 ttcttttggg atatatctac cgagacaccg ggaggaaaga agaggcaaag gaggcttgga
     1741 cccgggttct cgaattatac ccaaattttg ctcaagccga gaaaaatatc gaaagtctcc
     1801 aaaaatagct gcgccgagaa acggatagat caggtccctc acgccaatag cctttggttg
     1861 ttcactcaga gcgtctgaaa ttttttcgag cagaagctta tcccagtcac gccgttttac
     1921 ctctccaaac ttttccagat aggaaacgat ctctgcttat catgcacctt atcgaaggct
     1981 cgctttcgaa tatcctcagc ctgcgtttcc gttctgacgg caacctctgc tgagacgaaa
     2041 aagtcgggcc gacgcccttc gatcgatcgc ttggccctca atgttgaata ctcatcctcg
     2101 gtgagcggct tgcctttttg aatctttgcc cggggagccg gagttgacaa gggcggctcc
     2161 ctgggagaat atgaagcgga aaaggcttgc ggataaggag gacctttgca agaggcgctg
     2221 ggtaaatatc agatggccga ggcggtgcga gaggcctacg gctcggggga ggacgatgaa
     2281 accttggagc cccgggtctt gattgacgcc cggggaaatc ccatcggctg cttccgggac
     2341 ggggattaca ttatttttta caacctccgg ggggagcggg aggttgagct ttgccaaagc
     2401 cttctggata aggaattctc tcatttccct gtggaaagga atctccgtct aaacatggta
     2461 accatgatcc cctaccatca aaagctcaaa gcccgggtgg ccttctctcc cgaagaggag
     2521 atccgggata ctttgagtga aatcctgagc aggcacgggt taaggcaagt gaaggtctcc
     2581 gagtcggaga aagccattca cgtaagcttc tttctgaatg gcaaaaaccg caaacctttc
     2641 cccggggaag aaagaatcgc tgttcccacc cccaaaggcg ttgctcaatt tgaccagaag
     2701 ccggaaatga atgcctgcgg cgtagccgat tccgtgatcc aaagcttgca ggaccggcaa
     2761 gtgagtttct ggttcgccaa cttcgccaat atcgatgtgc tggggcatat tgaaaacgaa
     2821 gcagccatcc gaaaagcggt ggaggccgtg gacaacaacc tgggccgctg tctgaaagcg
     2881 gcccgccagg caggggtggt aaccatggtt accgccgatc atgggaccgc cgagaaatgg
     2941 ctctacccgg acgggaccat tgacacgggg cacaccgaca gccctgtccc ttttattctc
     3001 gttccccctc ggtctgaagg agcattctcc tggagtctgc gaagcagcgg tgggttgact
     3061 gatgtggctc ccaccgtctt ggaggtcctg gatatcccca aacctccggt catgaccgga
     3121 cagagtcttc tgctcaaaaa gccgatgaag aaaggttcgg gagaaaaccg ggtcctcctc
     3181 attttattgg atggatgggg atatcgttcg gaaactcacg gcaacctcat cgccaaggcg
     3241 cgcacgccgg tgatggacga tttgcagcag gggtatcctt tcaccaccct ggaagcttcg
     3301 ggggaggcgg tggggcttcc gcggggaacc gtgggaaact ccgaagccgg gcatctgcac
     3361 atcggagccg ggcgcagaat cttttcagac cgcctggtca tagaccgggc catccaggac
     3421 ggaacctttt ttcacaacga tgctttcctt tgggctatag ggggagccag gaaggaaggg
     3481 aaaaattttc accttatggg aatcgtctct ttcttcagct cccacggctc gatggaccac
     3541 ctgatggctc tgttcaagat ggccaaacag gaatcggttg aaaacctata tcaccacgcc
     3601 atgctggggc gcagagggga acggaaggaa agcggggcaa ggtacctgga gacgattgaa
     3661 aaagagctgg aaaatcttca tctgggaaga atcgtctcgg tcatcggccg ttactggtcc
     3721 ctggaccggg aggagaattg ggaccggatc gagaagacct accgcatgct ggtgtacggg
     3781 gaaggacaaa aagtccgctt gcgcccctga gggagcgcga attttagggg gaaaagaagt
     3841 tgacacatca aggtggcttt cctataattc tcccaccttc caggcgagtc cgtaccgaac
     3901 aaccatgaag aaggaggaac aagaacatga agctttttcg tatcctggtc gtcttcactc
     3961 tagtcctggg gctggtgtat gccgccactg cccaggccca gcagttcaac tggcagcaat
     4021 tcaaggggac acagattcgg gtcctcctga acaaacatcc cttccaggcg ggcatcgagc
     4081 cctccttgaa ggactttgag caactgaccg gcatcaagct aatcaccgaa gtctaccccg
     4141 aggatcagtt ccgggccaag gtgctggtcg agcttacctc cggcgccagc agcatcgacg
     4201 tgttcatgag catgccggcc caggagggac tcaagtacat acgggccggg tggctccagc
     4261 ccgtggacga attcctgaaa aacgcttctc tgaccgcacc cgattacaac tggaacgact
     4321 tcctggacaa gacccgggac gccatgacca tcgagggcaa gctggtgggc ccgcccatcc
     4381 aggtcgagaa tgccgccctg atgtaccgga aggacgtttt ctccaaatac ggcgtgaagg
     4441 tgcccacgac cctggacgag ctggaagccg cggccaaggc gctgaatggc aaggccatga
     4501 ccgacgacgg ccagcctgga ttcgggataa ccctccgtgg aaagcgggct gcagccacct
     4561 cgcagttcgc ggggatgctc cacgccatgg gctccacctg gctcacccca agccgggagc
     4621 cggccttcac gagtgatgag gggatcaagg cgttcgacat gtggggccga ctgctccgtt
     4681 tgtacggacc gcccggctcc gagaacaatc attggtacga ggcgtcctcg atcttctccc
     4741 agggaaaggc cgccatgttc gtggatgcca actccctctt cccggtgatt gaggacccgc
     4801 agaaatccaa ggtgatcggg aaggtgggat atgctttata cccgcgcggg ccgaaaggcc
     4861 agtatgggtc caccgtggca gtctggggac tggccatgcc caagagcagc aagaatcaga
     4921 aggctgcctg gctgttcatg cagtgggcca ccgccaaagc acaggttatg aacctccaga
     4981 gcgagaaagg catcctgggc tgccgggaat cggtctggaa ggacccgaaa gggaagggga
     5041 aaaccccggc ggacctggcc gagtcgctga tgcagggctc caagatggga actcccttgt
     5101 ggaacccgcc cgtcgtggcc gtggccgagg tgcgggacgc cgtgggggcc gccatcgtga
     5161 cctcgatcca gggcggcgac gtgaaagccg cagtgaacaa ggccgcggcg gacatgaaac
     5221 ggatcatggc ggagacggaa aagaagtagg ccgaaccttt atccaggaag gctggggagg
     5281 aggtccgcag ggacctcctc aaatttttcc catgaaatca tgacccagga gattcaaacc
     5341 gcaccagggc cgtcctcagt gggcgaggaa aagcccaatt cgtggtggtc gcacctggcc
     5401 gaaaagcacc ccgtcctgct cttcaccctt cctgccggga ttgtcgtttt tgtcttgatg
     5461 gtctttccgg tcttttacac gctctacatg agcctgcact cctggtttgc cagcagcgtg
     5521 acggccccgg aattcatcgg gctgcagaac ttcaagcggg cctttatcca ggacgaacgg
     5581 ttccgaaatt ccatcctcct caccctctat ttcaccatcc tggcaacggc catgcaactg
     5641 gttctcggcg tggccctggc tctcc
//
LOCUS       DASPRR010000145        15804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_686689, whole genome shotgun sequence.
ACCESSION   DASPRR010000145 DASPRR010000000
VERSION     DASPRR010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15804)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15804
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686689"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VEL68_05360"
     CDS             complement(<1..1118)
                     /locus_tag="VEL68_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ35428.1"
                     /translation="MNSRMTHRERVLAALNLQEPDRVPVDFGSTIATTIIQPAYEKLQ
                     NFLGIRHEIQFMARRARTVIPDERVLQRFDIDTRPVLLGDFKGGAYREIDSRTLVDVW
                     GITWKRAPEGHFINVDGPFQKVEPTLEVLERFEWPDPRTPSLYTGLKEKVEFLRKTTD
                     YALILNLPVGIVHHCQFMRGFGEWLMDLKLNPEFACALMDRIADVWIKIAEMAIDEVG
                     GDIDVISWGDDVAAQEAPFMSPQTYRELIKPRHKRMIQALKARCGAKVWYHSCGSVRL
                     LVEDFIDAGIDTLNPVQVSARNMDPVQLKKEFGNRLSFWGGIDTHRVLPFGTPDEVRR
                     EVREIIDCLGKGGGYLLASVHNIQAEIPPENIAAMFDEA"
     gene            1466..2521
                     /locus_tag="VEL68_05365"
     CDS             1466..2521
                     /locus_tag="VEL68_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ35429.1"
                     /translation="MKLTRRDFLQGSILGGSYLLLAPLPSLAEIIKRKEWQPGYAKLE
                     KEGKLTQRVKQARAIFERCELCPRKCGVNRLRGEKGFCQAPARAVIYSAHPHFGEEEP
                     ITGQNGSGTIFISNCNLRCVFCQNWPIAHEGRGREVSDEEFAGMMMDLQRIGCHNINI
                     VTPTHVMPNILGAVQIACRKGLRLPLFYNTSGYERVEVLKVLDGIVDIYKPDMKYSND
                     SLAEKYSSGARDYPEVARKAILEMHRQVGVLKSDENGFAVRGLLIRHLVMPNRIAGTE
                     AFVKWVSENLPKNTYVNIMAQYRVEYKAFEYPELARGITVEEFLEAMDSAKKYGLIDL
                     DSKSLAIKKIYEHRSSG"
     gene            2736..3536
                     /locus_tag="VEL68_05370"
     CDS             2736..3536
                     /locus_tag="VEL68_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ35430.1"
                     /translation="MGFETIKFKEPETGIGLITLSRPDRLNALSLDMVGELHALFLAL
                     MERKDLRILILTGEGRGFCSGADLKDARLRQEFPTSYPDPTVHLIAIQKRYADLVLEM
                     RHLSQPIIAAVNGAAAGGGMCLALASDIVIAGPKAAFTPSFINIGLSGGEMGTTFFLP
                     QKVGSGRAAEILLTGRTVDAQEAEKIGLVSRIVEENRLLEVAMEIAKTLLAKSPMGLR
                     FTKEALNLSLTAPSLEAVIEFENRNQSMCCSTPDFFKAVEAFMKRKRV"
     gene            complement(3619..4020)
                     /locus_tag="VEL68_05375"
     CDS             complement(3619..4020)
                     /locus_tag="VEL68_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXZ35431.1"
                     /translation="MIPQGMTVGRFFQKSREEKKVTLESVAKETRIKMDFLQAIEDDS
                     FQLIPCEAYIRGFIRNYAKSMHLNAEEVLDLYDLQVEPKKNQAAESADNSPSVSKSLN
                     QIKNHLFDFLTTMAGGSLAYPLGKTILRPKD"
     gene            4510..4896
                     /locus_tag="VEL68_05380"
     CDS             4510..4896
                     /locus_tag="VEL68_05380"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HXZ35432.1"
                     /translation="MKRLKKTLVWYLVGAMFVIGVTPKCFAGFSPSEVMALSQTERPQ
                     DLLKLQKFLETKMVRERLMDLGFIPEEVQMKLGNLSDQQIHQMALHLDEMKVAGDGVG
                     IFIAVFLIAVLVVLIIYATGHKIVFK"
     gene            5096..6106
                     /locus_tag="VEL68_05385"
     CDS             5096..6106
                     /locus_tag="VEL68_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ35433.1"
                     /translation="METSFYIQWHITNLCNLRCRHCYQVDFTKEQDLDWGGLRDVSDN
                     ILSALQAWEDKACIHLTGGEPLLKPELFPLLEHLDRQPGVEELGLITNSLLLTDEILD
                     RLSAVRKFKTVKISLDGADPPVHDRIRSAGNFERVVEKISLVRERGVFDLNLMFTVMR
                     SNLESLPAFLRLGEELRVDGMIIERFIPWGRGREVRNEVLGKEHWRSMLKALFAYFSM
                     ELEEDEIPPYQAFQVDFRGEEPQLLGAPCVLGKEGFCVMPNGDVFPCRRFPLTIGNLR
                     QDSFENIWQNCAVLNQLRRKENLKGKCGPCREENCTGCRSLAFSLTGDYLAEDPHCWY
                     GE"
     gene            6195..6674
                     /locus_tag="VEL68_05390"
     CDS             6195..6674
                     /locus_tag="VEL68_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXZ35434.1"
                     /translation="MSEKLHPSAQKVQEALTARGLPCQVVELPDSTRTAQDAAQAIGC
                     TVAQIVKSLVFRGTRTGKPILVLSSGVNRVSEKRMSEWAAEPIGKADADFVRRHAGFA
                     IGGVPPVGHPGPIETYIDEDLFQYDEIWAAAGTPYAVFRLTPKDLGKMGKGLVVTIK"
     gene            6682..7155
                     /locus_tag="VEL68_05395"
     CDS             6682..7155
                     /locus_tag="VEL68_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXZ35435.1"
                     /translation="MESNEAYEKKLKNFIKENQIFCEHLTFSECCHSVAEAAQAAGVT
                     PRDFVKNVCLVDARGSIVVAIVKGEDRVDMTRVAKVLEVKKVRIATAEEMLEKTGYPC
                     GGTPSFSYPARFLIDERVMEMDVVYSGGGSEYSLTRIRPADLVKANQGEMVRVRK"
     gene            7201..8358
                     /locus_tag="VEL68_05400"
     CDS             7201..8358
                     /locus_tag="VEL68_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ35436.1"
                     /translation="MACDLLVKNGQVFDPLKKVFQNVDIAVANGRVSRITPPLKGEHG
                     REVIDASGLIVTPGLIDLHVHIFHLVHRISIHPQSLIPRAGTTTMVDGGSSGAGNFDA
                     FREFILTKSDLNLLAFLNISLLGQVFELQIPGVPSMNEYEDLRLVNVSETVKCIAQNR
                     DFIVGIKVRAFHGLTNVTPIHAAVEAGEETGLPVMVHTAPPPLSVRQYFPFLRPGDIL
                     THLYHPSPGGLLDRRGKVRTEYREARERGVLMEVGLDRWHTDFEVLKRGMGEGFWPDI
                     ITTDVTRFNVEPLVRDVLFTASKIMAAGLPLPEALAAMTINPARAMKRPDLAELKEGG
                     PANIAVLEVLKGDVTFVDFFNQSMKGKERLVCRWLVNKGKILKEDRIAQNP"
     gene            complement(8434..9279)
                     /gene="nudC"
                     /locus_tag="VEL68_05405"
     CDS             complement(8434..9279)
                     /gene="nudC"
                     /locus_tag="VEL68_05405"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HXZ35437.1"
                     /translation="MKDNHFFISAVVPPKERSGPAWWFAFAGNQLLIHQESNSARIPY
                     LRNFSELKLPYHETHYLGELDGHSCYAVDLKENAEVPQGMALEGLRKVYGHLDEELFW
                     LAARAVQIVDWDRTHRFCGRCGAPLKARETERAKECQQCGLLHFPRLAPAVIVLVERG
                     NQLLMARSRHFAPGVYSVLAGFVEPGETLEEAVKREVLEEVGIQIRDVRYFGSQPWPF
                     PHSLMIGFTAQYAGGEIRVNDGELEDAGWFSVDRLPGQPGKISIARRLIDWFVAKQGG
                     SPLRD"
     gene            complement(9435..9917)
                     /locus_tag="VEL68_05410"
     CDS             complement(9435..9917)
                     /locus_tag="VEL68_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ35438.1"
                     /translation="MSEHNPKSGQEIFSPSSLCQIGIVVRSIDQTIQYYQEAFGFGPF
                     EIRDVDYPTATYYGETAGYRGKRAFFHLGPIQIELIELKDGKTIHESFLREKGEGLHH
                     IGFLVPDLKERKRRAERSGFKVIQGFNRSDGTGFAYLDSDKVGGVIFELIQRPEKNTA
                     "
     gene            10442..11992
                     /locus_tag="VEL68_05415"
     CDS             10442..11992
                     /locus_tag="VEL68_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ35439.1"
                     /translation="MGHPETKSSFPPGEDKDLYCRDLPSTPLAGMGKVLVTGASGYIG
                     GRLVPELLARGYRIRVMVRAPSPIYKERWPDVEIVTADALELNQLRTALKDVDTAYYL
                     IHSLLLGQREFAAADIRAAVHFRLAAEENRIKRIIYLGGLGDTQTKLSPHLQSRTQVA
                     EELIKGRIPVTVLRAATVIGSGSASYEIIQNLVRKLPIIFIPRWAMTRCQPIGIRDVI
                     KYLVGVLEIPETSGKSFDIGGDDVLSYYEMLKIRADLLNKKRFFLRFFSFLPLYSYLG
                     ALITTVPAPITQSLMKGLKNEVICQNREIQRYLPFQPLTYKEAILRAMNREEQDQIYS
                     RWSDAYPPAHELALKLHELERGPRYSSTYSLTTSKEAPSLFQCLSRVGGKEGWFSNNW
                     LWWLRGAIDKALLGVGTSRGRRSQTSLKINDVIDFWRVEDLQENRRLLLRAEMKMPGK
                     AWLEFRIHDEGFQRRLSINAYFDTASLWGRLYWYCFLPLHRFIFNRLIKQIEKRSAPL
                     LLDKKGPL"
     gene            complement(12267..12956)
                     /locus_tag="VEL68_05420"
     CDS             complement(12267..12956)
                     /locus_tag="VEL68_05420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ35440.1"
                     /translation="MSASEKTQLFDDWPDRYEKWFTTPIGALVKRVEWELIDDLLKPG
                     PGEFILDAGCGTGVFTLDMISCGARVVGLDLSLSMIQKARQKGASSQLRIISADILRL
                     PFLENSFDKTVSITALEFIPDGQKAIKELFRVTRKEGTLVVATLNSLSPWAERRREEG
                     QKGHSLFSKAFFRSPGELAALAPVKGESRTAVHFLKEEDPDRAGEIERKGKLKDLSTG
                     AFLAARWIKPC"
     gene            13197..13358
                     /locus_tag="VEL68_05425"
     CDS             13197..13358
                     /locus_tag="VEL68_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35441.1"
                     /translation="MLGIVMGSGATGGIVGPTLAGWVFDSMGSYQALWLAFAGAFSLA
                     VLLVLRVKP"
     gene            complement(13571..14434)
                     /locus_tag="VEL68_05430"
     CDS             complement(13571..14434)
                     /locus_tag="VEL68_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ35442.1"
                     /translation="MPKVGVNHIHLYYEEAGSGIPLIFVHEFAGDWRSWEAQMRFFSS
                     RYRAVTFSARGYPPSDVPESPEDYSQHLQIKDLKGLMDALRIEKAHLCGLSMGSYTSL
                     LFGLKYPEHCRSLTIAGSGYGSGANREDFHKQIVQVADQMLKGGMKAVSQTYSLGPAR
                     VQLQNKNPRAWDEFRRQFEEHSAKGSAYTFLGVQRKRPSITDLGDQLKKMALPALIML
                     GDEDERGLEGSIFMKKNIPRAGLEVFPRSGHTLNLEEPEHFNRSLLEFVTAVDAGHWE
                     ARDPRSLSPAV"
     gene            14592..14792
                     /locus_tag="VEL68_05435"
     CDS             14592..14792
                     /locus_tag="VEL68_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35443.1"
                     /translation="MKILLGGIVALIIGFVGLVIWWFEFLRVLKGVVPILFVLGGALA
                     VYLGIEDIKTTTASPPGEEKKE"
     gene            complement(14897..15331)
                     /locus_tag="VEL68_05440"
     CDS             complement(14897..15331)
                     /locus_tag="VEL68_05440"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HXZ35444.1"
                     /translation="MRKKPTQFLVLIISFALVGAFAHLALGQEKPKAAAASQEMVIAR
                     AVVGTGVENMEPVGAAESFPASTEKVFCFIEANNIPKDMEVSFVWSQGGKEVRKIDLP
                     LKAGPKWRTWANKNVGGQKGDWKVEIKDAEGKVLKEVKFKVE"
BASE COUNT         3741 a         4094 c         4290 g         3679 t
ORIGIN      
        1 gcttcgtcga acatggccgc gatgttctcg gggggaatct cggcctggat gttgtggacg
       61 ctcgccaaaa ggtatcctcc gcccttcccc aggcaatcga ttatttccct gacttcccgg
      121 cgtacttcgt ccggggtccc aaatggcaaa acccgatggg tgtcgatccc tccccagaat
      181 gacaggcggt ttccgaactc tttttttaat tgaacggggt ccatattgcg ggcgctcacc
      241 tgaaccggat tgagtgtatc aatacccgcg tcaatgaagt cctcaaccag caggcgaacc
      301 gaaccacaac tatgatacca gacctttgcc ccacagcgag cctttagggc ttggatcatc
      361 cgcttgtgtc ggggcttgat aagttctcga tacgtctgag ggctcatgaa aggagcctcc
      421 tgagcggcca catcatcccc ccaagagatg acgtcgatat ctcctcccac ctcgtcgatt
      481 gccatttccg cgatcttgat ccagacatcg gcaatccgat ccatcagggc gcaggcaaat
      541 tccgggttta gcttaaggtc catcagccat tccccaaacc cccgcatgaa ttggcaatga
      601 tggacaatgc cgacgggaag gttcaagatg agggcgtaat ccgtagtctt gcgcaggaat
      661 tcaacttttt ctttgagtcc tgtgtacaaa ctgggagttc tcgggtccgg ccattcgaac
      721 ctctcgagga cttccagggt gggttcaacc ttctggaaag gaccatccac attgatgaaa
      781 tgcccttcag gggcccgctt ccaggtgatt ccccacacat cgaccagggt gcgcgaatcg
      841 atctcccgat aggctccccc tttgaaatcg cccagcaaga ccgggcgggt gtcgatatca
      901 aacctctgga ggacgcgttc atcaggaatg acggtccttg cccgtctggc catgaattgg
      961 atttcatggc gaatcccgag gaagttttga agcttttcat aggccggctg gataatcgtg
     1021 gtggcgatcg tgctgccgaa atcgacaggg acccgatcgg gttcctgaag gttcagcgca
     1081 gccaaaactc tctccctgtg ggtcatcctg gaattcatct tttcagctcc catctctgag
     1141 gatgaaattc cccctttatt caccattctg aaaatgagcg cgtcagagtc tcctgcaggc
     1201 catttctaca tctggacggt tcaacaattc ttcgcttttc ccttccagcg ctttttagac
     1261 tcatgaattt tcaaatctta ggtttttgcc tgccccgatg agcgatgaga tagactagct
     1321 taatcccttt catttgtaaa atggaaaaga cctgctcgat cttgcccgtt tacttccttc
     1381 ctggttcctg gtaacattga atccgtccat ttcctgccaa gccctgccaa cgaaagtttt
     1441 ttccctcgga aaggggtgat tggatatgaa actcacccgc cgggattttc ttcagggttc
     1501 tattcttgga gggagctatt tgctcttggc cccgctcccc tctcttgccg aaatcattaa
     1561 aagaaaagaa tggcagcccg gctacgcgaa gctggaaaaa gagggcaaac tgacccagcg
     1621 ggtcaaacag gctcgggcga tcttcgagcg gtgtgagctt tgcccgcgga agtgcggagt
     1681 gaaccggctc aggggggaga agggcttctg tcaggcccct gcccgggcgg tgatctacag
     1741 tgcccatccc cacttcggag aagaggaacc catcaccggc cagaatgggt ccggaaccat
     1801 cttcatctcc aactgcaacc tccggtgcgt attttgtcag aactggccca tcgcccatga
     1861 gggacggggg cgggaggtga gcgatgaaga attcgccggc atgatgatgg acctgcagag
     1921 gatcggctgc cacaacatca acatcgtgac gcccacccac gtgatgccca acatcctcgg
     1981 ggcggttcag atcgcctgcc gcaaagggct gcgccttccc ctcttctaca ataccagcgg
     2041 atatgagaga gtggaggtcc tgaaggtcct ggatggaatt gtggatattt acaagcccga
     2101 catgaaatac tcgaacgact ccctggcgga aaagtactct tccggcgccc gggattaccc
     2161 ggaagtggcc cgaaaagcca tcctggagat gcatcgccag gtaggtgttt tgaagagcga
     2221 cgagaacggg tttgccgtgc gggggctgct catccggcat ttggtcatgc ccaatcggat
     2281 cgcggggacg gaagcattcg ttaagtgggt ctcggaaaac cttcccaaga atacctatgt
     2341 gaacattatg gcccagtacc gggttgaata caaagccttt gaatatcctg aactggcccg
     2401 ggggatcacg gtggaggaat tcctggaagc catggattcg gcgaaaaaat atggattgat
     2461 cgacctggat tcaaaatctt tggccatcaa gaaaatttac gagcaccgca gctcgggctg
     2521 aaggagtgaa ccccctggta agaccctcca acaaacatcc actttgccag tcacgcatcg
     2581 gcagcggcag accaaaggaa acattttgtg ggggcaaagg gaaccgcaga aatccctccc
     2641 gttcccctct ttttaaactg ggagcgggaa gattctcgga ggctggcttc cgaagggaaa
     2701 aagaaaaatt aaatgaatag aaagggaatg agaaaatggg attcgagacg atcaaattca
     2761 aggagccgga aactggcatc ggcctcatta ccttaagccg tcccgaccga ttgaatgcct
     2821 tgagcctcga tatggtcgga gaactccatg ctctcttctt agctttgatg gagcggaaag
     2881 atttgaggat tttgatcctc accggggaag gccgggggtt ctgctcaggg gcggacctga
     2941 aggatgcccg tctgcgacag gagtttccga cttcatatcc cgacccgact gttcacctga
     3001 tcgctattca aaaaaggtac gccgacctag tgttggagat gcggcatctg tcccagccca
     3061 tcatcgccgc cgtcaatggc gcggcagccg gcgggggaat gtgtctggcc ctagcatcgg
     3121 atattgtgat tgccgggcca aaggccgctt tcaccccttc gttcatcaac atcgggcttt
     3181 ccggggggga aatgggtacg acctttttcc ttccccagaa ggtggggagc ggccgggcgg
     3241 ccgaaatcct gctgaccgga cgaaccgtgg atgcccagga ggcagagaaa atcggcctgg
     3301 taagcaggat agtggaagaa aaccgtctcc tggaggtggc gatggagatt gccaagacat
     3361 tgctggctaa gagccccatg ggacttcgct tcacgaagga ggccctgaac ctgagtctga
     3421 ccgccccttc tctggaggcc gtgatcgaat tcgaaaatcg caaccagagt atgtgctgct
     3481 cgacccctga tttcttcaaa gcggttgaag catttatgaa gagaaagcgg gtataagagc
     3541 agggaataat atatcagctg tttttggcag tcaaaatctg aacaggtgcc gggaaagggg
     3601 ggaaggaagg atttccagct agtctttagg gcgcagaatc gttttgccca aggggtaggc
     3661 taacgatcct cccgccatgg tcgtcagaaa atcaaataga tgattcttga tctgattcag
     3721 tgatttgctc acagagggag agttatctgc gctctccgcc gcctggtttt ttttcggttc
     3781 aacctggaga tcgtataaat caagaacctc ttccgcgttc agatgcatgg acttggcata
     3841 attgcgtatg aatcctctaa tgtaggcttc gcatgggatc agctgaaagg aatcatcttc
     3901 tatggcctgg aggaaatcca tcttgatccg ggtttccttc gcgacggatt ccaaagtgac
     3961 ttttttctct tctcttgatt tttgaaaaaa tcgaccgacg gtcattccct ggggtatcat
     4021 ttgaatccac tctctaatag cgaggaaaaa taaatagcta ttgtacacat tgatatagca
     4081 taatccgggc caatttcctc ttggagatag aaaaaaaagg gaatatttgc ctttctaaag
     4141 aatccccttt aattaccgat ggttaaaaaa ataccggaaa ggagcaggga taatccatag
     4201 cttatttacc cgaaaataga aaagatttca aggaaaaaat tacctatttt tgtactccat
     4261 agtccaaaaa ttaccttttt tatcccgcct gtccccataa ttcccagaat tgctcaaatc
     4321 catccgctca agatagatgg aaaccaggca aagagacctg aagccgttct attcaagcgg
     4381 caaatggaag cgagcctgcc aaggtattga cctttgaccc atcatccgct agtcttggtt
     4441 aaggacgttc ttccaggttg acttttttga tcttcaggat ggtttgatgt caaaaaagga
     4501 gaatggggaa tgaaaagatt gaaaaagacc ctggtatggt acctggttgg ggccatgttt
     4561 gtaatcggtg tgacacccaa atgttttgcc ggattttccc cgtccgaagt catggctctt
     4621 tcccagacgg agagacctca ggaccttctg aagcttcaaa aattcctgga gaccaagatg
     4681 gtcagggaaa ggctcatgga ccttggattt atccccgaag aggtccagat gaaactgggg
     4741 aacctcagtg accagcagat tcatcagatg gctctccatc tggatgaaat gaaggtggcg
     4801 ggggacgggg taggcatatt cattgccgtt tttctcattg ccgtgctggt ggtcctgatt
     4861 atttatgcga cgggacacaa aattgttttt aaatgatccc ccatggccaa caggaaatca
     4921 ctttgggctt gccagtgaag ctgtgaaata tattttcagc cggtaacgat tctctaaaat
     4981 cgccttcaat cgctcttggc caagagggga gggggcgacc tctgaaaaga caccgacgtt
     5041 ccgcttcatt cttctgccgt gcccatggcg tgcggggagt cggtcgcggg tattgatgga
     5101 aacctctttt tacatccagt ggcacatcac caatttatgc aatcttcgct gccgccattg
     5161 ctaccaggtc gattttacga aagagcagga cctggattgg ggcgggctga gggatgtttc
     5221 ggacaatatc ctttcggctc ttcaggcctg ggaagataaa gcctgcatcc acctcaccgg
     5281 gggagaaccc ctattaaagc cggagctctt tcctctcctg gaacacctgg atcgccagcc
     5341 aggggttgaa gaattgggcc tcattaccaa tagccttctc ttgaccgacg aaatcctgga
     5401 ccggttgtca gccgttcgaa aattcaagac ggtcaagatt tctctggacg gggcggatcc
     5461 cccggtccat gatcggatcc gttcggcggg aaactttgag cgggtcgtgg aaaagatttc
     5521 cctggttcgg gaaaggggcg ttttcgacct caatctgatg ttcacggtga tgaggagcaa
     5581 cctggagagt ctccccgctt tccttcgtct cggcgaagag ctgcgggtgg atgggatgat
     5641 catcgaacgg ttcatcccct ggggcagggg aagagaggtc cggaatgaag tcctcgggaa
     5701 agaacattgg cgcagcatgc tgaaggccct cttcgcctat ttctcgatgg aactagagga
     5761 ggatgaaatc ccgccctacc aagcctttca ggttgatttc cggggggagg agccccagtt
     5821 gctgggcgcg ccctgcgttc taggaaagga aggcttctgc gtcatgccca atggcgatgt
     5881 tttcccctgc cggagatttc cgctgaccat aggaaatctc cggcaggatt cttttgaaaa
     5941 tatttggcag aactgcgctg tcctgaacca gctccggaga aaggagaatc tgaaagggaa
     6001 gtgcggtccc tgccgggagg aaaattgcac cggttgccgg agtctggctt tttccctgac
     6061 cggcgattac ctggcggaag accctcattg ctggtatggt gaataggcat ggaaggggcg
     6121 accttatccc gaacaggcaa ggaagggaag gaagcgggca gcaaagaacg atgggcatga
     6181 ggaggcaaga caaagtgtca gagaaactcc accccagcgc gcaaaaggtt caggaagcat
     6241 tgaccgcccg tggattgccc tgccaggtgg ttgaactgcc ggacagcacc cgcacggccc
     6301 aggatgcggc ccaggccatc ggttgcacgg ttgcccagat cgtaaaatcc ctcgtcttcc
     6361 ggggaacccg gactggaaag cccatcctgg tgctttcgag cggggtgaac cgggtaagcg
     6421 aaaaaaggat gagtgaatgg gcagcggaac cgatcggaaa ggccgatgct gattttgtgc
     6481 gccgacacgc cggatttgcg atcggaggag tgccccccgt gggtcacccg gggccgatcg
     6541 agacttatat cgacgaggac ctttttcaat acgatgaaat ctgggcggcg gcgggcaccc
     6601 cctatgcggt ttttcgtcta acgccaaagg acctggggaa aatgggcaag gggttggtgg
     6661 taaccatcaa gtgaaggaat tatggaatcg aatgaggctt atgaaaaaaa gttaaagaat
     6721 tttattaaag aaaaccagat attttgcgaa catttaacat tttccgaatg ctgtcactcg
     6781 gtagctgagg ccgctcaggc ggctggagtc actccccgag attttgttaa gaatgtatgt
     6841 ctggtcgatg cccgaggttc tatcgtagtg gcgattgtga aaggagagga ccgggtagac
     6901 atgacccggg tggcgaaggt cctggaggtc aagaaggtac gaattgccac ggcggaagag
     6961 atgctggaga agaccgggta cccctgcggc ggcacgcctt ccttcagtta cccggctcga
     7021 tttctcatcg atgaaagagt catggagatg gacgtggtat attccggagg gggctcagag
     7081 tactctttaa ccagaatacg gccggccgac ctggtgaaag ccaatcaagg agaaatggtc
     7141 cgagtcagaa aatagccctt tgatccgcca ttttcccaaa taacaaaagg gtgaggcgcc
     7201 atggcctgcg atctcctggt caaaaacgga caagtcttcg atcctctaaa aaaagtcttc
     7261 caaaatgttg acattgcggt tgcaaatgga agggtctcac ggattacccc gccgctgaaa
     7321 ggggagcatg gaagggaagt cattgacgct tccgggttga tcgtcacccc ggggctcatc
     7381 gaccttcacg tgcacatctt ccacctggtc caccggatca gcattcatcc ccaaagcctg
     7441 atcccccggg cagggaccac caccatggtg gacgggggaa gctcaggggc gggcaacttc
     7501 gatgccttca gggaattcat cctcaccaaa tcggacctca atctgctggc cttcctcaat
     7561 ataagcctgt taggtcaagt cttcgagctg cagattcccg gcgtcccttc gatgaatgag
     7621 tacgaggacc tgcgcctggt gaatgtgagc gagacggtga agtgcatcgc gcaaaaccgg
     7681 gatttcatcg tcgggatcaa agtccgggcc tttcacggac tgactaatgt taccccgatc
     7741 cacgctgccg tggaagcggg ggaggagacc ggcctgccgg tcatggtcca cactgccccc
     7801 cctccgcttt cggtaaggca gtactttccc tttctgcggc cgggggacat cctgacccac
     7861 ctttaccacc ccagccccgg tgggttactg gatcgccgcg ggaaggtccg cacggaatac
     7921 cgggaggccc gggagcgcgg ggttctcatg gaagtgggcc tggaccggtg gcacacggac
     7981 tttgaggtgc taaagcgggg tatgggggag ggattctggc cggacatcat caccaccgat
     8041 gtgacccggt tcaacgtcga acccctggta cgtgatgttc tattcacggc cagtaaaatc
     8101 atggccgcag gactgccctt gcccgaggct ttagcagcca tgaccatcaa cccggccaga
     8161 gctatgaagc gtccggattt ggccgaattg aaagaggggg gaccggcgaa tatcgccgtt
     8221 ctggaggtcc tgaaggggga tgtcaccttc gtggattttt tcaaccagtc tatgaagggg
     8281 aaagagcggc tggtctgtcg atggctggtc aacaagggca aaattttgaa ggaagaccgg
     8341 atcgcgcaaa atccgtaggg cagagagtga aagaaaaaaa acaatgggca gagaccaaaa
     8401 caaaaataca gggaagggga cgcggacgca aggtcaatcc ctgaggggag acccaccctg
     8461 ctttgccacg aaccaatcga tcaatcggcg ggcgatgctg atttttcccg gctgcccggg
     8521 cagtcggtcc acgctgaacc aacccgcatc ttccagttct ccgtcgttga cccggatttc
     8581 tcccccggca tattgggcgg tgaatccaat catgagggaa tggggaaaag gccatggctg
     8641 gctaccgaaa tagcggacgt ctctaatctg gattcccact tcttccagca cctccctctt
     8701 tacggcttct tccaaggtct ctccaggctc cacaaaaccg gccaagacgc tatagacccc
     8761 cggggcaaag tgacgggagc gggccatgag aagctgattc ccgcgttcca ccaggacaat
     8821 cactgccggg gcgaggcggg gaaagtggag aaggccgcac tgttggcatt ccttggcccg
     8881 ctcggtttcc cgggctttga ggggggcccc acaacggccg cagaaccgat gggtccggtc
     8941 ccaatccacg atctgcacgg cccttgccgc cagccagaac aattcctcat ccaggtgacc
     9001 ataaaccttg cgaaggcctt caagggccat tccttgggga acctctgcgt tttcttttag
     9061 gtccacggca tagcaggaat ggccgtccaa ttcccccagg tagtgggttt cgtggtaagg
     9121 aagtttcagc tcggagaaat tccgcagata ggggattcgg gcggaattgg actcctgatg
     9181 aataagcaat tgatttcccg cgaaggcgaa ccaccaggcc gggcctgacc tttccttcgg
     9241 gggcacgacg gcggagatga agaaatggtt gtccttcata atctgcagct tttccggcca
     9301 ggtattcccg gatggatttt agcggttctt ctttttttcg atcgagatta acactcctct
     9361 gttcctccct tccgcgattg aggaaagagg aacggaagga tgatattgag actctctgct
     9421 cttggcgttc tgccctatgc agtgttcttc tcaggccgct ggatcaattc gaatatgacc
     9481 cctcccacct tgtccgaatc caggtaggcg aacccggtcc catccgaccg gttgaaccct
     9541 tggattacct taaagccgga cctttctgcc ctccttttcc tttctttaag atcagggacc
     9601 aggaaaccaa tgtgatgcag cccctcgcct ttctcccgta aaaaggactc atgaatcgtt
     9661 tttccgtcct tcagttcaat taattcaatc tgaatcgggc ccagatgaaa gaacgccctt
     9721 ttcccccggt acccggcggt ttccccgtaa taggtggcgg tggggtaatc cacatctcgg
     9781 atttcaaagg ggccgaatcc gaaggcctct tgataatatt ggatcgtttg gtcaatactc
     9841 cgcacgacga ttccgatctg gcaaaggctc gaaggagaaa agatctcctg ccccgatttc
     9901 gggttgtgct cgctcatctt tcttttcctt tcccgcaggg tagctctaat tcgagtatag
     9961 aacaaaacct ccagaaggaa aaccccgtat caagaggaat aggaagttca ggggggcaaa
    10021 aagaatttcc agcggaggga aggggagact tataccgggg aaaaaagaac ccccgggcag
    10081 ggccctgggg gctcggaagg tccgcctatg ccgtcggatg cggaaggacg aaatctcttt
    10141 tcgaaggttc ccttctattc gtgttcgatt cgtctccgac cctcaacggg catcgcagaa
    10201 attccatcta tcgtatcggc ctatccagag gaacctttac atgaaaaaat ttccctggaa
    10261 actttccacg gggctcattc tttccccggg ttttggactg ggttcccagg cctaggtcta
    10321 ctgatgaaaa tcattcttcc aggtggattt attgaatatt ataggttgaa ggacgccccg
    10381 cagtcccgct gggtgaaaag ctgtaagaaa tgagaatcaa aagaaaaaga atcaaatagc
    10441 catgggtcac cctgaaacga agtcatcctt tcccccgggg gaagataaag acctttactg
    10501 ccgggattta ccttcgacgc cattggccgg catgggaaag gtactcgtga ccggcgcctc
    10561 tggctatatc ggggggcggt tagtccctga attgttagcc agaggttatc gaatcagagt
    10621 gatggtcaga gctccttcgc cgatttacaa ggaacgatgg cccgatgtag aaattgtcac
    10681 cgccgacgct ctcgaattga accagctacg aacagcccta aaagatgtcg atactgccta
    10741 ttatttgatc cattccttgc ttctgggtca aagggagttt gccgcagcag atatccgagc
    10801 ggccgttcat ttcagattgg ccgcggaaga gaaccggatc aagcggatca tttatctcgg
    10861 gggcctcggc gatacccaaa caaaactgtc gccccacctg cagagccgga cccaggtcgc
    10921 tgaggaatta ataaagggaa gaatcccggt cactgttttg cgagccgcta ccgttattgg
    10981 gtctgggagc gcctcctatg aaattattca gaatttggtc cgaaagctcc ccatcatttt
    11041 tatcccccgc tgggccatga ccaggtgcca gcccattggc attcgggatg tgatcaaata
    11101 tctggtcggg gtattggaga ttcctgaaac ctccggaaaa tcttttgaca tcggggggga
    11161 tgatgttctc agctattacg agatgttaaa aatccgggct gatctcctga acaagaagag
    11221 attttttctg cggttctttt ctttcttgcc cctttattca tacctgggag cccttatcac
    11281 gacggtgccg gcgcccatta cccaatcttt aatgaaagga ttaaaaaatg aggtgatctg
    11341 ccagaatcgg gaaatccagc gctacttgcc ttttcagccg ttgacttata aagaagccat
    11401 cctccgggcg atgaatcgag aggagcaaga tcaaatttac tcccgctggt ccgacgccta
    11461 tccgcccgcg cacgaactag ccctgaagct ccacgaactg gaaagaggtc cccgatattc
    11521 aagcacctac tctttgacca cctcaaaaga ggctccttcc ctttttcaat gcctaagcag
    11581 ggttggcggg aaagaaggct ggttttctaa caactggttg tggtggttgc gaggggccat
    11641 cgataaggcc ttactggggg tgggaacctc cagaggaaga aggagccaga ccagcctgaa
    11701 aataaacgac gttatcgatt tctggagggt tgaggacctt caagaaaata gacgcctctt
    11761 gctccgtgcg gagatgaaaa tgcccggaaa agcatggttg gaatttcgaa tccatgatga
    11821 aggcttccag agaaggctct cgataaatgc ttatttcgac acggcaagcc tatgggggag
    11881 gttgtattgg tattgcttct tgcccttaca ccgatttatc ttcaatcggt tgattaaaca
    11941 gattgaaaag agaagcgctc ccttgctttt ggataaaaaa ggacccctat aaatttgaaa
    12001 tcccccccgg tttcctggca ataacgatct ggcgggcttt aggaaggccc aacccccttt
    12061 ctttcttcat gtttttgccc ttctcgcata atcgaattca agacgatagg ccttcccggt
    12121 gacggtaggg tctgggtgag aatccaggtc gggcgcacga acatcccatc cgctcgtaca
    12181 ttcgcatcgc cacctgcatc atatcgattg tatgcaggtt caggctggat cctccaatta
    12241 atcatgtcta gactatcatg gaacccctag caaggtttga tccaccgggc agccagaaaa
    12301 gcaccggtgc tcaagtcttt taatttcccc tttctttcaa tttcccctgc tcggtccgga
    12361 tcttcctcct tcaagaaatg gaccgccgtg cggctctctc ccttcaccgg ggcgagagcg
    12421 gcaagttctc caggggaacg gaagaaagct ttggagaaca gggagtggcc cttttggcct
    12481 tcctcgcgcc tgcgttcggc ccaggggctc aggctgttca gagtagccac gacaagggtc
    12541 ccctccttgc gagtaacgcg gaagagttcc ttgatcgcct tctggccgtc cgggatgaat
    12601 tcgagggcgg tgatggaaac cgttttgtcg aaagaatttt cgaggaaagg gaggcggagg
    12661 atgtcagctg aaatgatcct cagctgggag ctcgcgccct tttgtctggc tttctggatc
    12721 atggaaaggg aaagatcgag gccgaccacc cgggccccgc aggaaatcat gtccagggtg
    12781 aaaacgccgg ttccgcagcc cgcgtctagg atgaactctc caggcccggg cttcaataag
    12841 tcgtcgatca attcccattc cacccttttc accaaggccc cgatgggagt tgtgaaccat
    12901 ttctcatacc ggtccggcca gtcatcgaag agttgggttt tttccgaggc ggacatagcg
    12961 ttttttctcc attatgccca accttatcat cgtcccttca aggggtcaac ccttgaaaat
    13021 tcggaatgcg gaatgtggaa cggggaatag tggaacaggg gaatgcaaaa tccgatgcgg
    13081 ggttgggtct tttcattgcg gatttcgcat tccgaattcc gcatgcgaat gggccattga
    13141 ctttacctcg ggatgaagtt ttaacgggct attcccggag ggattagctg gggaaaatgc
    13201 tggggatcgt catgggatcg ggggccacgg gaggaatcgt cggtccaacc ctggcaggat
    13261 gggtttttga ttctatggga agctatcagg ctctctggct cgcctttgcc ggggccttca
    13321 gcctggcagt gcttctggtt ctgcgggtca aaccgtaact cgtgggatca cgccgtcccg
    13381 cgaaacccgg ggtgcccctg cctgaaacct aagactcgaa atccgaagcg taaacccttt
    13441 gatttttgcc cagaaggtcc tagcccccgg acctgacgcc atatcgagga ccaccttcag
    13501 ggggcgccct tttcaatccg ctttgagcga ttccctgctt ttcatgcctc cgctccgccg
    13561 gaggttatga ttatacagcc ggggaaaggc tccgggggtc cctcgcctcc cagtggccgg
    13621 catccaccgc ggtgacgaat tccaggagag agcgattgaa atgctccggc tcctccaggt
    13681 tcagggtgtg gccgctgcgg ggaaaaacct ccagcccggc gcggggtata ttcttcttca
    13741 tgaagatgct cccttccagt ccccgttcat cctcatcccc cagcatgata agggccggca
    13801 gagccatctt cttcagttga tcccctaggt cggtaatcga gggccgcttc cgttgcaccc
    13861 ccaggaaggt ataagcagat cctttagcgg aatgctcctc gaactggcgg cggaattcat
    13921 cccaggcccg gggattttta ttctgcaact gaacccgagc cgggcccagg gagtaggttt
    13981 gggagactgc tttcatccct ccctttaaca tctgatcggc cacctggacg atctgcttgt
    14041 gaaagtcctc ccggttcgcc ccgctcccat acccggagcc ggcgatggtc aaggaacggc
    14101 agtgctccgg gtatttcaac ccgaataaaa gagaggtgta gcttcccatg gaaagcccgc
    14161 ataggtgggc cttttcaatc ctcagggcat ccatgagccc cttgaggtct tttatctgca
    14221 aatgctggga ataatcctcg ggtgattcgg gaacatccga cggcgggtag ccccgggctg
    14281 aaaaggtaac ggcccggtaa cggctggaga aaaaacgcat ttgcgcctcc cagctgcgcc
    14341 aatcaccggc gaactcgtgg acaaaaatca gcggaattcc gctgcctgcc tcttcataat
    14401 atagatggat atggttgacc cctactttgg gcatctcctc ctcctatctc tttggttgga
    14461 ccctacgggt atatctagca caggaagagt aggatttcca gcggttggtg agggggctcg
    14521 ggagaaaatt cttgatttga cagacggccc aagattcact aatatcataa aaaaagaaga
    14581 aaggaggaga tatgaaaata ttgcttggcg gaattgtggc cctgatcatc ggtttcgttg
    14641 gcctagttat ttggtggttt gagtttctgc gagtcttgaa aggagtcgtt cccatcctct
    14701 tcgtcctggg aggggccctg gccgtttacc tgggaatcga agatatcaag accacaaccg
    14761 cttccccgcc cggggaggag aagaaagaat aaattctttt ctgtgatttc aggaaagcgg
    14821 ccctttctat cctgcaggga agaaagggcc tcctagattt ggaattccag gtgggcggtg
    14881 ggggatgatt cagtccctat tccaccttga atttcacttc ttttaaaacc tttccctcag
    14941 catccttgat ttcgaccttc cagtctccct tctgtccgcc cacattctta ttagcccagg
    15001 tcctccattt ggggcccgcc ttcaggggaa ggtcgatctt cctaacctct ttgcctccct
    15061 gggaccagac aaaagagacc tccatgtctt tggggatgtt gttggcttcg atgaagcaaa
    15121 aaaccttttc ggtggaagcg ggaaaactct ccgcagcccc caccggctcc atattttcca
    15181 cccctgtgcc caccaccgca cgggcgataa ccatctcctg ggaggctgct gcggctttgg
    15241 gtttttcctg gcccagagcc aggtgggcaa acgccccgac cagagcaaag gaaataataa
    15301 gaaccagaaa ttgcgtgggt ttctttctca tctttttccc tccttcctga tttttgggaa
    15361 cccatggata attctattct atacgaaacc agattatttc tcaagctggg cgtggttctt
    15421 atctcgcccg ggcaaaagaa tgatcaggaa agatttatcc cttgaattta ttgccccggg
    15481 aagttacatt caataagttc cggcaaggga tttaaaaaaa tgaaagtcct ctgccggaag
    15541 ggatcggtag caaaagcttc aagaaaatca acctcgcctg aatccccatg aaaatagacc
    15601 tgctggaggc aatccatgga gccgtcagga ataagagggt ctccaaaggc ccgcttcatc
    15661 gggagcggtg cccaatgggg agtcggaagg aattgagatg ggcaagagct gggtagaaga
    15721 gaggaagacc gaagatttgt ggtgcgagaa cggggggcaa ggtgaaaatg attatggatt
    15781 tttcatcgct aaaagcaaat aaaa
//
LOCUS       DASPRR010000146         7435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_523559, whole genome shotgun sequence.
ACCESSION   DASPRR010000146 DASPRR010000000
VERSION     DASPRR010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523559"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VEL68_05445"
     CDS             <1..256
                     /locus_tag="VEL68_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35445.1"
                     /translation="ARPRLLLIDEPSLGLSPIIMGQIYEAIKTFPRDGITVLLSEQNA
                     LDALEIADRGYVLQDGHMVLQGPGGELLNSEMVKKAYIGM"
     gene            373..1752
                     /locus_tag="VEL68_05450"
     CDS             373..1752
                     /locus_tag="VEL68_05450"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ35446.1"
                     /translation="MADDYFDFTGKMFDPKTIPEKPEALKGIRVLDLSHMIFGPTAAR
                     FLAQYGAEVIKVEVPYQGDYWRGSTYWGKYWKHANPLWHFINHSKYFAAIDVKKPKGK
                     ELILKLAKLSDVVIENFTSGTVEAWGIGYSQVSKFNPRVIYASLSTYGQFGPMRFLPG
                     WDLLAQGTSGVLSLTGHPETDKFFKVPDFFGDFFPGYFASMMILMALHYRERTGEGQY
                     LDVCQAEILMRSLFHFTHLQMTGKELGRTGNLDPTMAPSGIFKTKDGKFVALAVATDG
                     QFQSLCQAMDRSALAVDPRFRKATDRLKEENARDLNGIVQNWVTSRTASELIALGRRH
                     RFAASEVMDDVQISQDPWRRERGNVVTHEDEMYKTMVLQGPLSMLSKTPGRIKWLTRP
                     LGYHNRYLLKKMLGLKEAEIRRLEKERVVGYWDYRVGQRPPVYYDIDRDPIFHYSSPE
                     ETPAGERTR"
     gene            1834..3189
                     /locus_tag="VEL68_05455"
     CDS             1834..3189
                     /locus_tag="VEL68_05455"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ35447.1"
                     /translation="MAIKEKVYSRYLQKKGTARQISAWGQILSQLTSLEGKPEALEDM
                     VVLDLSYANFSGSIASSFLAEAGAEVIKAEPPEGDPARVMTPFGVQVQGVGTPYLMES
                     RNKRHITLDWNSPEDRENLKRLAARADVLIETLAPGEMDSRGLGYRQLREINPGLVYV
                     AISPYGHYTARGKEFAEMPWTDLTSQAESGLAALIGDLADSPEPYSWPTRAGFYAAGY
                     TTAVAAATGALVAAFFKRRTGEGQMVDIAAADAYSSCVGFPPTIGDIWKKPRPRYGTL
                     DYGLCPYGFFKCKDGYVAIACFRDQDFRAALKIFGRWKLEEDWKSLLDRITDDIEKVR
                     GLNEEVEKVVAHYTYQEISEKFSAYSIRAARSKWRGGGLPVTTKMLTPREVMDQEHWQ
                     ARNTFARVEHPKFGIVTLPVTGKMSRTPLRVKWISADIGGDNEYIFKKYGLTRKESGK
                     S"
     gene            3323..4885
                     /locus_tag="VEL68_05460"
     CDS             3323..4885
                     /locus_tag="VEL68_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXZ35448.1"
                     /translation="MSFEKEMEELRRRKEKALRMGGPEKVNRQHEKGKLTARERIARL
                     LDPDTFFEVGMLNTSDIPGMEDKTPADSKVGGFGKIDGRTVAVVANDFTVLAATSSRV
                     AGHKEGEIKGQAVRRGFPTIYLGEAGGARMPDIMGAKGLASIGGGGFDTFLQLMSRVR
                     KSPMITAIMGECYGMPTWMACLSDFVVQVKGSAMAVSGPRVLELAISEKISDEDLGGW
                     KVHAQVTGNSDRVAENEEECFAIIRDFLSYMPSHCDEPPPRKPVPEGSGSRMDRVLDL
                     LPEKRNRVYDMNKILQTIVDGGILFPLKPLFGKSVITSLARIDGRVVGIVANQPWFNA
                     GAMETDGIDKVMSFLVLCDSFNIPVLFFHDTPGFLVGKEAERKRVGARVMNYMNVLGQ
                     MTVPRVAVIVRKTYGMAYWNLGGSGCATDFLVAWPTAEMSFVDPEIAANVVFAGKIQE
                     TPELAAQKEKFIQQMIEDSRPYPAAGMHYIHDVIDPRQTRRYLIRALEISEDRRTGGV
                     GQHRLANWPTKF"
     gene            4953..6491
                     /locus_tag="VEL68_05465"
     CDS             4953..6491
                     /locus_tag="VEL68_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXZ35449.1"
                     /translation="MDEYLKDLEERKAKALKLGGQAKIDLQHSLGRYTARERIERLVD
                     SGTFLELGMLNTSDVPGIEEKTSGDGMIGGLAKIDGRPVVVSASDRTVLAATEGMVHI
                     RKHHALHDYAINRGFPLFHLGEGGGLRIPDGMGSDGISQRMMPVNLLRHGRQVPLIAS
                     IMGDSFGGPTWFAVSADLAVQVKGTCMAVSGPRMLEIATSEKITPEELGGWKLHAETT
                     GQVDLFAENDDHCLEILKKVFAYFPSNAQEEPPFQSTGDDPDRQVEEVTKIVPTQRNR
                     AYDMKRLIRALVDGGIFLELKECFGKALLIGLARLNGRAVGILANQPLFNAGAAGPQE
                     CDKAVEFICLCDSYHIPMIFLHDIPGFLVGSFAEKNRMPTKIMVWNQAVAWSTVPKIS
                     VVIRKSIGAAYSNMCGPTLGADFVVAWPTAQISFTGDEVGVNVVYGRQLMEAENPAEE
                     RKKMLARWAYDSLPYRAAAMHLIDDVIDPRDTRKFLCQALDFACAKNGSKGRRLLANW
                     PTGF"
     gene            6574..>7435
                     /locus_tag="VEL68_05470"
     CDS             6574..>7435
                     /locus_tag="VEL68_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ35450.1"
                     /translation="MEQITHRFIETNGIRMHIAERGKGPLVVLTHGFPELWYSWRYQL
                     VALANTGFHAVAPDQRGYGQTDRPGPIEDYDIFRLTGDIVGLANALGEDRAVIIGHDW
                     GSVVAYHCALFRPDLFRAVALLSVPFLPRVGGKIPPTEIMKKVYGEEIFYQVYFQEPG
                     KAEADMEADIRKTMVSILFSLSGSAPPEKRWRYIYRKSERLLDAYFMPEKLPEWLAGR
                     DVTFFVQEFQRTGFRGGLNWYRNIDRNWSLTPFLEGAKLRQPTLFIAGDLDPVIRMYR
                     KPVESLEMSVP"
BASE COUNT         1608 a         2181 c         2229 g         1417 t
ORIGIN      
        1 ggcccgcccc cgcctgctgc tcatcgacga gccgtccctg ggcctgagcc cgatcatcat
       61 gggccagatt tatgaggcga tcaagacctt cccccgggac ggaatcaccg tgctcctgtc
      121 cgagcagaac gcgctcgatg ccctggaaat cgccgaccgc gggtacgtgc tccaggacgg
      181 gcacatggtc ctccagggac cggggggaga gctcctcaac agcgagatgg tcaaaaaggc
      241 atatatagga atgtagaaat agtttcgaat ttcgagtttc aggtttcaag tttcgggttt
      301 cggggttcgg gtcgtgcttt cgcttttaac ccaaaactcg aaactgggac ggtttgatta
      361 tcggagttgg gaatggccga cgattacttt gactttaccg gaaagatgtt cgacccgaaa
      421 acgattcccg aaaagcccga agccctgaag ggaatccggg ttctggacct ctcccacatg
      481 atcttcggcc ccacggcggc caggtttctc gcccagtacg gcgccgaggt catcaaggtg
      541 gaagtcccct accaggggga ttactggagg ggaagcacct actggggaaa atactggaag
      601 cacgccaatc ccctctggca cttcatcaac cacagcaagt acttcgcggc catcgatgtg
      661 aagaaaccca agggaaaaga gctcatcctc aagctggcga aattgtcgga cgtagtgatc
      721 gaaaatttca cctccgggac agtggaagcc tgggggatcg ggtattccca ggtgagcaaa
      781 ttcaatcccc gggtcatcta cgccagcttg agcacctatg gccagttcgg gcccatgcgt
      841 ttccttccag gctgggacct tctggcccag ggaaccagtg gggtcctttc cctcaccggg
      901 cacccagaga cggacaagtt cttcaaggtc cccgactttt tcggcgattt cttcccgggc
      961 tatttcgcct ccatgatgat cctcatggcc ctccattacc gggagagaac gggggaaggg
     1021 cagtacctgg acgtatgcca ggccgagatt ctcatgcgct ccctctttca ctttactcat
     1081 cttcaaatga cgggaaaaga actgggacgg acaggaaacc tggaccccac tatggccccc
     1141 tcggggatct tcaaaaccaa ggatggaaag tttgtagccc tggccgtggc cacagatggg
     1201 cagttccaat ccttatgcca agccatggac cggtccgccc tggcggtgga cccgcgtttc
     1261 cgcaaagcca ccgaccggct caaagaggaa aacgcgcggg acctgaatgg gatcgtgcaa
     1321 aattgggtta cttctagaac cgcttctgaa ctgatcgccc tcggccggag gcaccgcttt
     1381 gccgcatccg aagtcatgga tgatgttcag atcagccaag acccctggcg gcgggaaagg
     1441 ggaaacgtgg ttacccacga agatgaaatg tacaaaacca tggtcctgca aggccccctg
     1501 tccatgttga gcaagactcc cggccggatc aagtggctga cccgcccgct gggataccac
     1561 aaccgctacc tcctgaaaaa gatgctgggc ctgaaggaag cggagatccg gcgtctggag
     1621 aaagaaaggg tcgtgggtta ctgggactac cgggtcgggc agaggccccc cgtttattat
     1681 gatatcgata gggatccgat cttccattac tcttccccgg aagaaacccc ggcgggagag
     1741 cgaactcggt gacggtagcc ttgcgcgaat tcggaatttg gagggcggat tcggaacctc
     1801 ttttgattcg gcgtttccgc ggtgacggaa gtaatggcca ttaaggaaaa ggtttattct
     1861 cgctatctgc agaagaaagg gacggcccgg cagatatcag cctggggaca gatcctttct
     1921 caactgactt cccttgaagg aaagcccgaa gccctggagg atatggttgt cctcgacctt
     1981 agctacgcca acttctctgg aagcatcgct tccagtttct tggccgaggc cggggccgaa
     2041 gtgatcaagg ccgaacctcc cgagggggac ccggcaaggg ttatgactcc cttcggggtc
     2101 caggtgcagg gagtaggcac tccctacctg atggagtccc ggaacaaacg ccacatcacc
     2161 ctggactgga attcccccga agaccgggaa aacctgaagc gcctcgcggc ccgggccgat
     2221 gtccttatcg agaccctcgc cccaggggag atggattccc gggggctggg ctaccggcag
     2281 ctccgggaga tcaacccggg actcgtctat gtggccattt ccccttacgg ccactatacc
     2341 gcccggggca aagagtttgc cgaaatgccg tggaccgacc tgaccagcca ggcggagtcc
     2401 ggcctggccg ccctgatcgg agacctggcc gattctcccg aaccctacag ctggccgacc
     2461 cgcgccggct tttacgccgc cggctatacc acggctgtgg ccgcggcaac gggggccctg
     2521 gtggcggcct tcttcaaacg ccgcaccggc gaaggccaga tggtggatat cgccgccgcc
     2581 gatgcttact cctcctgcgt cggatttcct cccaccatcg gggatatctg gaagaaaccc
     2641 cggccccggt acggaactct cgattacggc ctgtgtccct atggcttctt caagtgcaag
     2701 gacgggtacg tggccatcgc ctgtttccgg gaccaggatt tccgcgcggc cctgaagatt
     2761 ttcgggcggt ggaaattgga agaagactgg aagtctctcc tggaccggat taccgacgac
     2821 atcgaaaagg tgaggggcct gaatgaagaa gtagaaaaag tcgtcgccca ttatacttac
     2881 caggaaattt ccgagaaatt ctccgcctac agcataagag cggcgcgctc caagtggagg
     2941 ggcgggggcc ttccggtgac caccaagatg cttacccccc gcgaggttat ggaccaggaa
     3001 cactggcagg cccggaacac cttcgcccgg gtggagcatc ccaaatttgg catcgtcacc
     3061 ttgccggtta ccggcaaaat gtccaggacc ccgctccggg tcaagtggat ttcggcagac
     3121 atcggcgggg acaacgagta tattttcaag aaatacgggt tgactcgaaa agaatccggg
     3181 aaatcgtagg ggcggcctgc gacgagctca gtcgagcgtc ggcgaaccgt tcctgacccg
     3241 cacgacgcgg aattcccgca ggggcattca tgaatggccc ctgccctttc aggatcgaat
     3301 cattttcaac aggggaactg ccatgtcatt cgaaaaagaa atggaagaat tgagacggcg
     3361 gaaggagaag gctctccgga tgggagggcc ggagaaggtc aaccgccagc acgagaaggg
     3421 aaagctcacc gcccgggaga ggatcgcccg cctgctggac ccggacacgt tcttcgaagt
     3481 gggaatgctg aacacctccg acataccggg aatggaagac aagacccccg ccgacagcaa
     3541 ggtcggcgga ttcggcaaga tcgacggacg gaccgtggcc gtggtggcca atgatttcac
     3601 cgtcctggcc gccacctcca gccgggtggc gggccacaag gagggagaaa tcaagggcca
     3661 ggctgtccgg cgcggtttcc cgaccatcta cctgggagag gccggtgggg cccgcatgcc
     3721 ggacatcatg ggcgccaagg gactggcctc catcggagga gggggtttcg ataccttcct
     3781 ccagctcatg agccgggtgc gaaaatcccc catgatcacc gccatcatgg gcgaatgtta
     3841 cggcatgccc acctggatgg cctgcctttc cgattttgtg gtccaggtga agggaagcgc
     3901 aatggcggtg tccggcccgc gggttctgga attggccatt agcgagaaga tcagcgacga
     3961 ggacctgggc ggctggaaag tgcacgccca ggtcaccggc aacagcgacc gggtggcgga
     4021 gaacgaggag gaatgtttcg ccatcatccg ggatttcctc tcctacatgc cttcccactg
     4081 cgacgagccc cctccgcgta aaccggtccc cgagggctcg ggcagccgca tggaccgcgt
     4141 cctggacctg ctcccggaaa agaggaaccg ggtgtatgac atgaacaaga tcctccaaac
     4201 catcgtggac ggaggaattc tcttccccct gaaacccctc ttcggcaaga gcgtaatcac
     4261 ttccctggcc aggatagacg ggcgagtcgt gggaatcgtg gccaaccagc cctggttcaa
     4321 cgccggggcc atggagacgg atggcatcga caaggtcatg agctttctgg tcctgtgcga
     4381 ttccttcaat atcccggtcc tcttcttcca tgataccccc gggttcctgg tgggaaaaga
     4441 ggcggagcga aagcgggtgg gtgcccgcgt gatgaactac atgaacgttc tgggccagat
     4501 gaccgtgccc cgggtggcgg tcatcgtgcg caagacttac ggaatggcct actggaacct
     4561 aggcgggtcg ggctgcgcca cagactttct ggtggcctgg cccacggcgg aaatgagctt
     4621 cgtagaccca gaaatagcgg cgaacgtggt cttcgccggc aaaatccagg agaccccgga
     4681 actggcggcc caaaaggaga agttcatcca acagatgatc gaggacagcc ggccctaccc
     4741 ggccgcgggc atgcattata ttcacgacgt gatcgaccct cggcagaccc ggaggtacct
     4801 catccgggcc ttggagatca gcgaggaccg gcgaacgggg ggggtcggcc agcaccgact
     4861 ggccaactgg cccaccaaat tctgaatcgt ttcgagtttc aggtttcagg ttcttccaac
     4921 tcgaaacttg aaacccatga agggtttttt tcatggatga atacctgaaa gatctcgagg
     4981 agcgtaaagc caaggccctc aagctgggcg gtcaggccaa gattgacctc cagcattccc
     5041 tggggcgata caccgcccgg gagaggattg aaagactggt cgattctggg accttcctgg
     5101 agctgggcat gctcaatact tcagatgtcc cggggatcga ggagaaaacc tcgggcgacg
     5161 gcatgatcgg gggtctggcc aagatcgacg gccgtccggt ggtcgtcagc gcttcggaca
     5221 ggaccgttct ggcggcaacc gaaggaatgg tccacattcg caagcatcac gctctgcacg
     5281 actatgccat caaccggggg ttccccctgt ttcatctggg ggagggcggg ggattgagga
     5341 ttcccgatgg aatgggttcc gacgggatca gccagcgaat gatgcccgtc aacctcctcc
     5401 ggcacggacg gcaggttccc ttgatcgcca gcatcatggg ggatagcttc ggagggccca
     5461 cctggtttgc cgtctctgcg gatcttgccg tccaggtcaa ggggacctgt atggccgtct
     5521 ccggtccccg catgctggaa atcgccacct ccgaaaaaat cacccccgaa gaactgggag
     5581 gctggaagct tcatgccgaa accaccggac aggtggacct cttcgccgag aatgacgatc
     5641 actgcctgga gatcctgaag aaggtctttg cctatttccc ttccaacgcc caggaagagc
     5701 ctcctttcca atccaccggg gatgatccgg accggcaggt cgaagaagtg accaaaatcg
     5761 ttcccaccca gaggaaccgg gcctacgaca tgaagcggct catccgggcg ctggtggacg
     5821 gcggaatctt cctggagctg aaagaatgct tcggcaaagc cctcctcatc ggcctggccc
     5881 ggcttaacgg ccgggctgtg ggaatcctgg ccaaccagcc cctgttcaat gccggcgccg
     5941 ccggccccca ggagtgcgac aaagccgtgg agttcatctg cctctgcgac tcctaccata
     6001 ttcccatgat cttcctccac gacatccccg gctttctggt gggaagtttt gccgagaaga
     6061 accgcatgcc caccaagatc atggtctgga accaagccgt ggcctggtcc acggtgccca
     6121 agatttccgt ggtcatccgc aagagtatcg gcgcggccta cagcaacatg tgcggcccta
     6181 ccctgggagc cgacttcgtg gtggcctggc ccacagccca gatcagcttt accggggacg
     6241 aggtcggcgt gaatgtggtg tacggccggc agctgatgga agcggagaat cccgcggagg
     6301 agagaaaaaa aatgctcgcc cggtgggcct acgacagtct cccttaccgg gctgcggcga
     6361 tgcatctgat tgacgacgtc atcgatccaa gagacacccg gaaatttctc tgtcaggcct
     6421 tggacttcgc ctgcgccaag aacgggtcca agggccgtcg tcttctggct aactggccca
     6481 cggggtttta gaaagtttcg agtttcgagt tttgggtttg gggttcccgt aacttgaaac
     6541 ttgaaacttg aaactcgaaa ctggggtttt agaatggaac agatcaccca ccgcttcatt
     6601 gaaaccaacg gcatccgcat gcacattgcg gaaaggggga agggcccgct ggtagttctc
     6661 acccacgggt tccccgagct ttggtactcc tggcgatacc agctggtggc tctggccaac
     6721 accggcttcc acgccgtggc tccggatcag cgcggctatg ggcaaacgga ccggccaggc
     6781 ccgatcgagg actacgatat tttccggctc accggcgata tcgtgggctt ggcgaacgcc
     6841 ctgggggaag accgggcggt gatcatcggg catgactggg ggtcggtcgt ggcttatcac
     6901 tgcgcccttt tccggcccga ccttttccgc gcggtcgccc ttctcagcgt tcccttcctg
     6961 ccccgggtgg gaggaaaaat tccccccacg gaaatcatga agaaggtcta tggggaagaa
     7021 atcttctacc aggtctattt ccaggaaccg ggcaaggcgg aagcggatat ggaagccgac
     7081 atccgcaaga cgatggtcag catcctgttt tccctctcgg gcagcgcccc tccggaaaag
     7141 cgatggcgat acatttaccg taaatcggaa aggctcctcg acgcgtattt tatgcccgaa
     7201 aagctgcccg agtggctggc cgggagagat gtcaccttct ttgtccagga attccagcgg
     7261 accggtttcc gcggcggcct caactggtac cgaaacatcg accggaactg gagtcttacc
     7321 cctttccttg aaggagccaa gctccggcag ccaactcttt tcatcgccgg agacctggac
     7381 ccggtcatcc ggatgtatcg caagccggta gaatcgctcg aaatgagtgt tcccg
//
LOCUS       DASPRR010000147         7450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1650486, whole genome shotgun sequence.
ACCESSION   DASPRR010000147 DASPRR010000000
VERSION     DASPRR010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650486"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..664
                     /locus_tag="VEL68_05475"
     CDS             299..664
                     /locus_tag="VEL68_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXZ35451.1"
                     /translation="MIYRMKEKGFTLIELMIVIAIIGILAAIAIPQFAAYRNRGFNAT
                     SLSDIRHMITAQEAYFAERQIYIAPGDVAVSLSAYGYARSSNVTATIVSSNSFTYQMT
                     AAHGSGDRTWTFLSPGPIQ"
     gene            787..3018
                     /locus_tag="VEL68_05480"
     CDS             787..3018
                     /locus_tag="VEL68_05480"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HXZ35452.1"
                     /translation="MGWVDPGSGFKKSINTLLGFLLFIFTFSFFLGNLCPSLYWRDPG
                     EFAAIAFTLSVGHPTGSPTYSLIAKLFTFLPFGSLWQKINLVSVVFGSLTISIGFLLI
                     LRLLRKDDADFPQKQTFAFFSAAAASLLLAFSPTFWLYSLVAKAYPYLTFWILILVWL
                     LLGFREEAQKSPSPLPLSPQGQSYFFAAAFLYGLGLGVYGAMALYLPAFLFYCFYTDR
                     RWYRDPKILFLGGFFFLLGFSVYLYLPLRSSTNPFLDWGEPRTFSRFLNHVMDAKDAS
                     HNASFPDWTIIGKMSLDSLWLLSDQFSPFGLFLGGIGLIFLWHRDKACFSLTLGIALI
                     HWLFFVRIWKMAFLYLPLFLIFTLWMGTGIFFLGKAIAFRPWKNARPGFQRAMSWAWA
                     GVIVFTLIFQYALHKPSSGKGDYYVAYALGKEMLGSLPPKTLLFSNYSTMLFHGLQAV
                     ENFRSDVSVAVIFPLRNPHLFWSIDPAHYPAFDYSKLPRVVHPNTQEFFQTLITTHLG
                     KYPLFWDLGAEDNWLIPRLVPKGLLYHIQEKEMAASQGELSTDQKKTYEFYQFLFSLE
                     GFEEDEEGRQLLKNLTNMKSIYFMRTGFPSFALSYNRIAMQLFEDDPTLHNNRGIIYH
                     SMGKNEEALELLQKAVDRYPEDPLARLNRGAVFEAQGENAQALKEWKEASRLGGRGVL
                     GYYNRGKLLLKMGKTEEALSEFNAFMEKSWVLLYLSDHPILEEVKERVKEIEKRIQEK
                     KNS"
     gene            3372..4976
                     /locus_tag="VEL68_05485"
     CDS             3372..4976
                     /locus_tag="VEL68_05485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXZ35453.1"
                     /translation="MDVRGRKNCGPMSGGEKIILCTLGLLFFFNLILAFAPPTSRDAL
                     TQHLALPKLFVRHHGVYNIPFAEASFNPLQVDMLYTLVLLLGAEECASLLHFTFALLT
                     GALIYLLLLKSVPRWMALLGPLLFLSTPLVVNLSSKAYVDLGLAFYAFAAIYALLKKE
                     GKENWATISALFAGLAAATKYNGFLVLLLLWVWFLLESQKENFSSSLRGARRYLLIAL
                     AVSFPWLLRNFFYTGNPVFPFFVTWFGSDLPMEQEAFAPLARRALLYGEGWTDFLTIP
                     IRMFFQGQDEIPRYFDGVLNPILLFLSPLALMGIREGWRVRMAGFVILYFLLSLFLVD
                     MRARYILPTLAPLAVLSVFGLVTLRSLPAGKLWVASSLALLLLNFFYLQDFLCRADLG
                     LYLRGKESKEEYLSRTLSDYDLWRYANEHLPEDARVMMYFLGNRGYYCDREYVYESYY
                     SGKRLKQVLSRSPSVERLVEYFREGRITHIALRKDLFFQFAEANLLPAEKEAWGKFRN
                     CHLRLLFEGKGYQLYQVQGGLDASGA"
     gene            4960..5910
                     /locus_tag="VEL68_05490"
     CDS             4960..5910
                     /locus_tag="VEL68_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ35454.1"
                     /translation="MHLGRKVGVVVPAYNEEKLIERTIRTMPSFVDRIIVVDDCSRDR
                     TVEIVQNLAQEFGARLVLVRHERNQGVGRTIVTGYQRAIEEECEVTAVMAGDAQMDPR
                     DLIRIIEPVAKREADYVKGNRLFRGESWQMIPKHRYLGNSALSFMTKVASGYWHIADS
                     QSGYTAISLIALKRLELDRVYPRYGMPNDILVRLNIHNFKVRDVSVRPVYNIGEKSGI
                     RLYKVIPTISWLLFKGFWRRMFRKYVIQDFHPLIFFYLLAFGALALSAVFFFRTLYYW
                     IDLGSVPTTSFLVFWFLLISGFQSLFFAMWFDMENNRHLR"
     gene            5918..6700
                     /locus_tag="VEL68_05495"
     CDS             5918..6700
                     /locus_tag="VEL68_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35455.1"
                     /translation="MSRDFTLAKYRELLEALQEAGYRMLSVRDFLVQKGPLPCIILRH
                     DVDRREGRALAMALLEKDRGIQSTYYFRVGRGLGKPEVVLRIKALGHEVGYHYEALSK
                     AGGDREKARKVFGDELSRLRQMAEVRTAAMHGRPFSPWNNLSLWETCCPEDFGLMGEA
                     YLSFGRLSRAVYLSDTGRGWNSGENLRDRFSPEGPAMPGPFSSTGELIRFLRENPFPM
                     VYLLVHPNRWTSGTGSWLLQWGEDACVNWLKRKLKRHASTRS"
     gene            6705..>7450
                     /locus_tag="VEL68_05500"
     CDS             6705..>7450
                     /locus_tag="VEL68_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXZ35456.1"
                     /translation="MKVLLVNPPYLPEERYGNLAAFGPTNEPLGLAYIAGSLAEAGVK
                     VKIADAPALRMSVEEIGNWVVRDRFDLVGVTMLTPMYHRSLEVLRQTRRVSPAIRTVA
                     GGPHPTVLPEQTLQEIPELDFAVIGEGEITSVELVEALEGKRGLDSVAGLTFRKDGRV
                     VANPIRPPMRNLDQLPLPARHLLPMDAYRMTRSRTQTEHSYTVSVARGCPFRCAYCFR
                     TFGRAFRPHSPARIIQEISLLVDQYGAKEI"
BASE COUNT         1513 a         2007 c         1977 g         1953 t
ORIGIN      
        1 atgttgccgt aactttgagc gcctacggct atacccgctc cagcaatgtt accgcaacca
       61 ttgtgtcgtc taactctttc acctatcaga tgacgaactc acacggctcg ggggaccgaa
      121 cctggacgat cacaggccct ggcggaagca tccaatagcg aaggtcgcct ggccagttcc
      181 gtcgccccac ttggaattgg cctaagatgc tacctgggaa tgggtggggc ctggtgaagg
      241 gaccacccat tccctcataa atcatctcca taaacaaaac gacgtagcgg ggtttggaat
      301 gatttaccgg atgaaagaaa aaggtttcac gctgattgag ttgatgatcg ttatcgctat
      361 catcggcatc ctggcggcca tcgctattcc ccagtttgct gcctaccgga acaggggttt
      421 caatgccacg tccctgtccg atatacgcca tatgataacc gcccaggaag cgtacttcgc
      481 agaaaggcag atatacattg ctcccggtga tgttgccgta agtttgagcg cctacggcta
      541 tgcccgctcc agcaatgtta ccgcaaccat tgtatcctcc aattctttca cctatcagat
      601 gacggcggca cacgggtcgg gggaccgaac ctggaccttc ttaagtcctg ggccgatcca
      661 gtaatttttc cgcatgtgtt tttgagtact cgttgggcaa aatcgaaaat ttctccgaga
      721 aatgggcgga atcctccgta ggagaggata gcccatttct tttttggggg aaggaaagcg
      781 gagttcatgg gatgggttga tcccggcagt ggtttcaaga aatccataaa tacccttctt
      841 ggattccttc tttttatctt taccttctct tttttcctgg ggaatctctg cccatctctg
      901 tactggaggg acccggggga gtttgccgcc attgctttta ctttatccgt aggccatccc
      961 acaggttcgc cgacctattc cctgattgcc aagctcttca ctttccttcc ctttgggagc
     1021 ctatggcaaa aaattaacct cgtttccgtg gtgttcgggt ctctgacgat ttccattgga
     1081 ttccttttga ttttgcgtct cctcaggaaa gacgatgcgg attttcccca aaaacaaacc
     1141 ttcgcttttt tctccgccgc ggctgcttct ctgctcctgg ctttctcccc cactttttgg
     1201 ctctattccc tggtagccaa ggcctatccg tacttgacct tttggattct gattcttgtc
     1261 tggctccttc tgggctttcg agaggaagcc cagaagagcc cttcccccct ccccctttct
     1321 ccgcaagggc agtcctattt ctttgccgcg gctttcctct acggtttagg actgggagtc
     1381 tacggggcca tggcgcttta tcttccggcc ttcctttttt actgttttta caccgatcgc
     1441 cgatggtacc gcgatcccaa aatccttttt ctgggaggat tttttttctt gttaggcttt
     1501 tcggtatacc tctacttacc ccttcggtct tccaccaatc ccttcttaga ttggggagaa
     1561 ccgcggacct tttcccgctt cttgaaccac gtgatggatg ccaaagatgc ttcccataac
     1621 gcttccttcc ccgattggac cataatcggg aagatgagtc tagacagcct ctggctgctg
     1681 tccgatcaat tttctccctt tggactattt ctgggaggga taggccttat ttttctttgg
     1741 cacagagaca aagcctgttt ttccctcacc ctgggaatcg cgctgatcca ttggcttttc
     1801 ttcgttcgga tctggaagat ggcgttcctt tacctcccct tatttttgat ctttactctc
     1861 tggatgggga cgggaatctt tttcctgggg aaagcgatcg ctttccgccc ttggaaaaac
     1921 gcgcgccctg gattccaaag ggcaatgtcc tgggcttggg cgggggtgat cgtgttcacc
     1981 cttatcttcc agtatgccct tcataagccc tcttcgggaa aaggggatta ttacgtagcc
     2041 tacgcactgg gaaaagaaat gctcggttct ctgcccccca agaccctcct cttctcaaac
     2101 tattccacca tgctgttcca tgggcttcag gccgtggaga acttccggtc ggatgtttcc
     2161 gttgcagtca ttttcccttt gcggaatcct catctttttt ggagcatcga cccggcccac
     2221 tatccggcct ttgattactc caaactgccg cgggtggtcc atccgaatac gcaggaattt
     2281 ttccagacgc tgattaccac ccacctgggg aaataccccc tcttctggga cctcggcgcc
     2341 gaggataatt ggttaattcc ccggcttgtt ccgaaagggt tactctatca catccaggaa
     2401 aaggagatgg ctgcttccca aggtgagttg tccacggatc agaaaaaaac ttatgaattt
     2461 taccagtttc ttttctccct agaaggattt gaggaggatg aagaagggag gcagctcctc
     2521 aagaacctga ccaatatgaa gagcatctac tttatgcgca cgggatttcc ttccttcgct
     2581 ctatcttata acaggattgc catgcagctt ttcgaagatg accctactct gcataacaat
     2641 aggggcatca tataccattc catggggaaa aatgaggaag ccctcgagtt gttgcagaag
     2701 gccgttgacc gatacccgga ggaccccctg gcgcgcctga accggggggc cgtatttgag
     2761 gcccaaggag aaaatgcgca ggcattgaag gaatggaagg aggcctctcg gttaggaggg
     2821 agaggagtcc taggttatta taaccgagga aaattgctgc ttaaaatggg aaaaacagaa
     2881 gaggccctga gtgaattcaa tgcttttatg gaaaaatcct gggtcctatt atatttatct
     2941 gaccacccca tcctagaaga ggtcaaggaa agggtgaagg aaattgaaaa gcgtattcag
     3001 gagaagaaga actcttagag acctggatgg aaagaacatt ggggatagtt gatctcagta
     3061 caacatcttt aaacctattg ggcttggata cctggtcaga atcaatcggg aggattcctt
     3121 ggcttcgctg cttgtccatc cttggaatat ggactttctt gaacctttcg gccttgggag
     3181 cgggaagact aataggggcc agactaaaag cctcctttga gtcaggtccc gaatcgctgg
     3241 ttttgaaact gggcctcggt tacggcgcgt tgggctatgg tgtccttctg atagggttag
     3301 gaggtgctct ctatcctctg gtcctctggg tcctcctggc gtttttgatt cccttctctt
     3361 tcctggggag gatggatgtc agggggagga aaaattgcgg ccccatgtcc gggggagaaa
     3421 aaatcattct ttgcactctc ggcttattgt tcttcttcaa tcttatcctt gcctttgcgc
     3481 cgcccacgag tcgcgatgcg cttacccaac atctggcgct ccccaagctt ttcgtccggc
     3541 atcacggggt ctacaatatt ccttttgcgg aagcttcttt caaccccctg caggttgaca
     3601 tgttatatac cttggtcctc ctcttggggg ccgaagaatg cgccagcctg ctccatttca
     3661 cctttgccct cctgaccgga gccctgatct acctcctttt gcttaagtcc gttccccgct
     3721 ggatggcttt gctcggaccc cttttattcc tcagcacgcc cctggtagtc aatttgtctt
     3781 ccaaggccta tgttgatctg ggcttggcct tttatgcctt tgcggccatt tatgcgcttc
     3841 tgaaaaaaga ggggaaagag aactgggcga ctatctccgc tcttttcgcc ggattggcag
     3901 cggcgaccaa gtataacggc tttttggttc ttctcctgct ctgggtctgg ttcctcctgg
     3961 agagccaaaa agaaaacttc tcctcatcgc ttcggggggc aaggagatac cttctgattg
     4021 ctctggcggt ctcttttccc tggttgctcc gtaacttttt ttacaccggc aaccccgttt
     4081 tccccttttt cgtgacttgg ttcgggagcg atctgccgat ggagcaggaa gcctttgccc
     4141 ccctggctcg tcgggccctt ttgtacggag agggatggac ggatttcctg accataccga
     4201 tccggatgtt ttttcagggg caggatgaaa ttccccgcta cttcgacggg gtcttgaatc
     4261 ccattttatt attcctttct cccctggctt tgatgggaat ccgagaaggc tggcgggtcc
     4321 ggatggctgg gttcgtgatc ctttactttc tcttatcctt gttcctcgtg gatatgcgcg
     4381 ctcgctacat cctgcccact ttggccccgt tagccgtcct ttcggttttt ggtttggtca
     4441 ccttgcgatc ccttcccgcg gggaaactct gggtggcctc ttctcttgcc cttctgttac
     4501 tcaacttttt ctacctccag gacttccttt gcagagccga tcttggtctt tatcttcggg
     4561 ggaaagaaag caaggaggaa tatttgagcc gaaccctgag cgattatgac ctctggaggt
     4621 atgccaatga gcatcttccg gaggatgcaa gggtgatgat gtacttcctg gggaaccggg
     4681 gatactactg cgatcgggaa tatgtttacg aatcctacta cagcgggaaa agactgaagc
     4741 aggttctttc ccgttctcct tccgtcgaaa gactggtaga atatttcagg gaaggaagaa
     4801 ttacgcatat tgctcttcgg aaagacctat tttttcagtt tgccgaagcc aacctcttac
     4861 ccgcagaaaa ggaggcatgg gggaaattcc ggaactgtca cctgcggctg ctgtttgagg
     4921 gaaaagggta ccaactctac caggtccagg gaggcctcga tgcatctggg gcgtaaagtg
     4981 ggagtagtgg tcccggccta caacgaggaa aagctgatcg agcggacgat ccggaccatg
     5041 ccttcctttg tggaccggat catcgtagtg gacgactgca gccgggaccg cacggtggaa
     5101 attgtccaga acctggccca ggaattcggg gccaggctcg tcctggtccg ccacgaacgg
     5161 aaccagggtg tgggccggac cattgtcacg ggttaccaac gggcgattga agaagaatgc
     5221 gaagtgactg cggtcatggc cggagacgcg caaatggacc cccgagactt gattcggatc
     5281 atcgaacccg tggccaagag ggaagcggat tacgtgaaag ggaaccgcct cttccgggga
     5341 gagtcctggc agatgattcc caagcaccgg tacctgggga attcggccct ttccttcatg
     5401 accaaagtcg cctcgggata ctggcatatc gcggattctc agtcgggcta cacagccatc
     5461 tccctgatcg ccctgaagag gctggagttg gaccgggtct acccccgcta cggcatgccc
     5521 aatgacatcc tggtgcggct gaacatccat aatttcaagg tgcgagacgt ttcggtccgc
     5581 cccgtctaca acatcgggga gaagtcggga attcggctct acaaggtgat ccccaccatc
     5641 tcctggcttc tcttcaaagg attctggcgg cgcatgttcc gcaagtacgt gatccaggat
     5701 tttcatcccc tgatcttttt ttacctcttg gctttcgggg ccctagccct gagtgcggtt
     5761 tttttcttcc ggacccttta ttactggatc gacttggggt ccgttcccac gacttccttt
     5821 ttggtcttct ggtttctgct gatctcgggt tttcaatcct tgttcttcgc catgtggttc
     5881 gatatggaga acaaccggca cctgcggtga ggagactttg tctcgggatt ttactctggc
     5941 caaatatagg gagcttttgg aagccttgca ggaggccgga taccggatgc tgtcggttcg
     6001 ggatttcctt gtccagaaag gacccctacc ctgcatcatc ctacgccatg acgtggaccg
     6061 ccgggaagga agggcgctgg ctatggccct tctggagaag gaccggggga ttcagtcaac
     6121 ctactatttt cgcgtgggca ggggtctggg aaaacctgag gtggtgctcc ggatcaaagc
     6181 cctggggcac gaggtgggat atcactacga ggctctctcc aaggccgggg gagaccggga
     6241 gaaggcccgg aaagtcttcg gcgatgaact ctctcggctt agacaaatgg cagaggtgcg
     6301 gactgcggcc atgcacggtc gtcctttctc cccctggaac aacctatctc tctgggaaac
     6361 ctgctgtccg gaagactttg gccttatggg agaggcctac ctttctttcg gccgcctttc
     6421 cagggctgtc tacctgagtg acacggggag gggctggaac tcgggagaga acctgcggga
     6481 ccggttttcc cctgaagggc cggccatgcc gggtcccttt tcctctacgg gggaattgat
     6541 ccgttttcta agggaaaacc cctttccgat ggtctatctg ctggttcacc ccaaccgctg
     6601 gacttcgggg acaggctcct ggcttctcca gtggggggaa gatgcctgcg tcaactggct
     6661 gaagagaaag ctcaagagac acgcttcgac tcggagttga aagaatgaaa gtccttttgg
     6721 tcaatcctcc gtatttaccc gaagaacgct atggcaacct ggccgccttc ggtccgacca
     6781 acgagcccct ggggctggcc tacatcgccg gcagcctggc agaggccggg gtaaaggtca
     6841 agatcgcgga tgccccggca ttgcggatga gcgtcgagga aatcggcaac tgggtcgtga
     6901 gggatcgatt cgacctggta ggggtgacca tgctcactcc catgtatcat cgcagcctgg
     6961 aagtcctgcg ccagacgcgc agagtttccc cagcgatccg aaccgtggca ggggggcctc
     7021 atcccacagt cttgccggag caaaccctgc aagaaatccc ggagctggat ttcgcggtca
     7081 tcggggaagg ggagattacc tcggtagagc tagtagaggc gttggaaggg aagagaggtc
     7141 ttgattccgt ggctggattg actttccgga aggacggcag ggttgtagcc aatcccattc
     7201 gcccgcccat gaggaatttg gaccagcttc cgctgccggc gcgccacctt ctccccatgg
     7261 acgcctatcg gatgacccgc agccggaccc agacggaaca tagttacaca gtgagcgtgg
     7321 cccggggatg tcctttccgc tgcgcctact gttttcgaac ctttggacgg gcctttcgcc
     7381 cccactcgcc ggcccgcatc attcaggaaa tttcccttct ggtggatcag tatggagcca
     7441 aggagatcaa
//
LOCUS       DASPRR010000148        12280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1974725, whole genome shotgun sequence.
ACCESSION   DASPRR010000148 DASPRR010000000
VERSION     DASPRR010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974725"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VEL68_05505"
     CDS             complement(<1..297)
                     /locus_tag="VEL68_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ35457.1"
                     /translation="MIYGIQARKGFFSIIGVVGTGKTTLIRHLLSTLDPKIKTVFIPR
                     TFLTFEELLEEILSKLELPLGNKSKFFLIHQLNNYLIRRLSRNETLVLIIDGAHN"
     gene            complement(584..1663)
                     /locus_tag="VEL68_05510"
     CDS             complement(584..1663)
                     /locus_tag="VEL68_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HXZ35458.1"
                     /translation="MKSYKIVVIRGDGIGPELVDATLEVLEKVQENAGDFRLSYEFHE
                     AGANYYQKNGVNISPETLEAIRKADAAMKGPVGLPGVRLPDGTEAGVLGGVLRSGFDL
                     YANVRPIKLFPKVAIPLKDRPPERIDYVIVRENTEGLYLSRGRGIITEQAASDTLLMT
                     RKGCERVCRFAFRLAVEKSKGAPEDGRRRVTLVDKSNVLRSFYFFRQIFTEVAKEFPS
                     VDAENLYVDAASAALVMRPEHFQVVVTENMFGDILSDLGGATIGGLGMCPSANMGDQR
                     AYFEPIHGSAPDIAGKNRANPLSQVLAAGMMLNYLGRKKDALLLEKAVRQALEKERFR
                     ISSIGQVEGGARQVVSALKEELEKN"
     gene            1897..3435
                     /locus_tag="VEL68_05515"
     CDS             1897..3435
                     /locus_tag="VEL68_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35459.1"
                     /translation="MVKDSKMNPSPFLSLRGISKSFRAVKANDQVDLDVYRGEVHALL
                     GENGAGKSTLMKVLYGFYHADSGEVRLDGKTVHIRSPHDARRLRIGIVFQDFVQVPAL
                     TVAENIALFLPDLPVVLDKLDILKRIGEISHRYSLAVDPRAPVRQLSVGERQKVEILK
                     LILADAQILILDEPTRSLALHEIEGLFRVFANLRRDGYAVVFITHKLKEVLTCADRIT
                     VMRRGKVTGTLAGSEAIETRLVSLMFEEEVRETSRIERTRAPGGVEPILQLRGVHTSG
                     EGGAVGLQDIDLAVLPGEIVGVAGVSGNGQRELGDVVLGLERCSKGEKYILGQEATHW
                     SVARARANGLAFIPEDPLGMAAFPWLTVQENMAMADTRRYSRKNGFSMDWVAVCADLD
                     QSLQRLGFTIPSFFVPLATLSGGNVQRMILAREMAHNPKLIIAFYPTRGLDVRSAVAA
                     RELLIASRDAGAGVLLISEDLGELFVMSDRLVVIFRGRIVKSCPPQETNPNEVGHLMT
                     GSGQ"
     gene            3437..4486
                     /locus_tag="VEL68_05520"
     CDS             3437..4486
                     /locus_tag="VEL68_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35460.1"
                     /translation="MREARKSAGSFPAQGDGWLSAMVRFGGLFLFALGAFALVLLVFG
                     KNPIKAYVDIFANTLGSAYGFSEVLVKMIPLTLAAVAVALPARIWLINVGGEGQFHIG
                     ALLAAWGALNFGNLPAWVLIPWISFLGFLGGGLWGLISGFLRARGWVSETISTLLLNY
                     VAILLLSFFIFGPWKDPESANYPQSAPFADAAVLPSFFGTRVHMGLFYSLTALVFYHL
                     ILSRTRWGLEMRAIGANAEAARRNGISIGRYIMILMFIGGGLAGLAGMAEVSAIHGQL
                     RPSLSPGYGYIGFLISWMAMGNAVGMMAAAFLLAIITAGGDILQMTLRIPGSVVNILM
                     AIILFVILARKGGGR"
     gene            4483..5400
                     /locus_tag="VEL68_05525"
     CDS             4483..5400
                     /locus_tag="VEL68_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35461.1"
                     /translation="MIEPILTGLLVGSIHSGTPLLYATLSEVVGERAGIVNLGLEGVM
                     LVGAVVGFATTALTGNAVLGVLAAALAGGLFNLFFGFLVITRRANQLASGLAMMFCGV
                     GLSALLGVSFVGSKIKGLDEVGIPVLSDIPLAGPMFFNYDILVYLAGPIAVLVYWVLF
                     FTRWGLSLRAVGENPTAAFAAGKNPHLLQYQALFLAGIMGGIAGAHLSIALAKTWQEW
                     MTAGRGFIAVALVIFSKWHPLRAIFGSLLFGGAIALQLQMQAWGVPISPFLLDMLPYL
                     LSLGVLAVWGGARRLAAPGSLGRVFQGTE"
     gene            5449..6549
                     /locus_tag="VEL68_05530"
     CDS             5449..6549
                     /locus_tag="VEL68_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ35462.1"
                     /translation="MRRERGAWVRPLGILASLILVLNFGFPEAAPAQAKLKVGVLHIG
                     SMTDGGYNQAHAEGIAVMKKNLPEVEVIEVENVPETADSERVMESMIKQGAKLIVAAS
                     FGYLDPTLRVAKKYPDVKFVHPAGYKRAPNLTTYWASTPEAFYLMGIAAGTTTKTNKM
                     GFVCALPVSFFLANVNALHLGARSVNPKVETRIVFTGTFLDPGKEATATNALLDQGSD
                     VMGMIVDSPITVVQTAEKRGAYSVGYHYLGVQKFAPKGWIGGVAFTWGGLYTRFAKQV
                     MDGTWKSENILGSLASDMITLAPFGPAVSPEAIKMVNDVKKSFLDGKKEVFAGPIKDN
                     KGAERVKAGEHWSTDNLGKMDWLAEGIIGQTK"
     gene            7106..7570
                     /locus_tag="VEL68_05535"
     CDS             7106..7570
                     /locus_tag="VEL68_05535"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HXZ35463.1"
                     /translation="MARAKDSTLELARNCLQQDLLRCGIGGRELAGLGPGLTPSGDDF
                     MGALFFAARSLHQAYPGDFPWAEDAISELLDWAKTRTHPVSYTIFRDLVLGHGPAALH
                     DFFAMLLQERDSCSVLNAAISLTRIGHSSGWDMLAGALTGLLMVKRSLENRA"
     gene            7648..8907
                     /locus_tag="VEL68_05540"
     CDS             7648..8907
                     /locus_tag="VEL68_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HXZ35464.1"
                     /translation="MTLTQSDIKQKIEVANAEAVKRIHEADPVLVDVAPAGEVIPGLK
                     DRMILHSGPPVTWEKMCGAQRGAMIGVALFEGWSKTPEEAEKVLQSGAIQFEPNHHHQ
                     AVGPMAGTISVSMPVWVVENRAFGNRAFCRQVEGRQQFGEYSDEALEGLRLWRNVWAP
                     SLRKGLLKRGGLELKPLIAKALQMGDELHNRPMAATSLFANAMAVAMVEADVAKEPLL
                     GTLKYITNHELLCLGLSMAAGKASADPATGIEFSTVVVAMARNGTEFGVRVSGLGEEW
                     FPAPSPRVNGLYLPGYSEKDAGCDMGDSAITETVGWGGFVLAGATGILALVGGTPEEA
                     MKYSQDMRQITVSASPNYRMPIFGFQGTPVGIDIRKVVQTGITPIIDTAIAHKNPGYP
                     IIGAGLVRAPLECFQKALKAFGQKYMK"
     gene            9104..10669
                     /gene="fdrA"
                     /locus_tag="VEL68_05545"
     CDS             9104..10669
                     /gene="fdrA"
                     /locus_tag="VEL68_05545"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HXZ35465.1"
                     /translation="MVTGCVIRKKEYHDSVFLMRVARRISEQKSVLQAAALMGTEKNK
                     VLLADIGIGGKEIEAAGPGDLIVAVKADDGETLSAMLGNLDSWLQLSSGPPGATPFCT
                     LEEALRRQPHSNLAVISVPGEYAAREARRSLERGLNVFLFSDHVPVKDELALKEYARE
                     KGLIVMGPDCGTALIGGKGIGFANAVRRGPIGVIGASGTGVQEFTTLVHHSGSGISHA
                     IGTGSRDLADEIGGISLLSALGSLESEPQTKVIVILAKPPGKRTLANILPKIQACSKP
                     VVACFLGETKNFQQEDHSFQVARTLNEAAAAAVQIAAGSEKLPILAEPQVSRIRLQGE
                     KAAMKPGQKYIRGVFAGGTFCYQSQQILRDAGIRVSSNIPLEGNSELPNPHSSLKHTL
                     VDMGADEFTSSCPHPMIDSSQRRERILAEAQDPEIAVLLLDFILGFNASADPAGELLQ
                     AITRAKEEVGKRGGFLCVVASVCGTDEDFQGFAKQVKLLQGAGAVVFPSNAQSAAFCA
                     ALIQSLQEGPDGR"
     gene            10659..10832
                     /locus_tag="VEL68_05550"
     CDS             10659..10832
                     /locus_tag="VEL68_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010137748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fdrA domain protein"
                     /protein_id="HXZ35466.1"
                     /translation="MADKIRELLDHGPVVINLGVKDFAESLKGQDVEVAHVDWTPPAG
                     GDREMIELLDQLL"
     gene            11059..>12280
                     /gene="pgi"
                     /locus_tag="VEL68_05555"
     CDS             11059..>12280
                     /gene="pgi"
                     /locus_tag="VEL68_05555"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXZ35467.1"
                     /translation="MLKKIDPTKTDAWRDLSNHFSKIQGVHMKDLFAEDPRRFERFSL
                     RFKEILVDYSKNRITPETIKLLLSLAAEVDLRDAVEKMFTGDKINETESRAVLHVALR
                     NRSNTPVYVDGQDVMPQVNAVLEKMKSFSEKVIFGDWKGFTGRRVSDLVNIGIGGSDL
                     GPVMVTECLKPYARQGLSVHFVSNVDGTHIGETLKKLNPETTLFLIASKTFTTQETMT
                     NAFSARDWFLARAQDPRHVARHFVAISTNREKVRSFGIHPDNMFTFWDWVGGRYSLWS
                     AIGLSIACYIGFENFAEVLQGAHEMDRHFRETPLEKNIPVILALIGVWYNNFFGAETE
                     AILPYDQSMHRFPAYFQQGNMESNGKSTDRNGKRVSHQTGPIIWGEPGTNGQHAFYQL
                     IHQGTKLIPADFLAP"
BASE COUNT         2596 a         3343 c         3626 g         2715 t
ORIGIN      
        1 attatgggct ccatcgatga tcaggaccag agtttcattc cgcgagaggc gccggatcaa
       61 gtagttattg agctgatgaa tcaggaaaaa tttgctttta tttcccaggg gcaactccag
      121 tttcgaaaga atttcctcca gcagttcctc gaaagtaagg aaggtccgag ggatgaatac
      181 ggttttgatc ttggggtcca gggtgctcag aagatggcgg atgagagtcg tcttgccggt
      241 tccgactacg ccgatgatgg agaaaaatcc ttttcttgcc tgaattccgt agatcattga
      301 agccagagtt tcccgatggc tgctagtcaa gaataggaat cgaggatggg gagttgggtg
      361 aaatgggttt ccggaaatgc cataaaattc attgatcatt ttgacggcca ttccctggga
      421 tcgcaaatca atattttcga gctaccagtc tcaattaaac cgatcggttc tgggttgaga
      481 ggaacgcctt aacccgagtc ccatgggttt cataaattaa agaagccctc ttgccttcct
      541 tcctttaccc cccaattcgg cctatctttc aggggtccac tcttcagttc ttctccagtt
      601 cttccttgag agccgaaacg acctgacggg cgccgccttc cacctggccg atggaggaaa
      661 tccggaagcg ctccttttcc agggcttgcc gaactgcctt ttccaggagg agagcgtcct
      721 tcttccgtcc cagatagttt agcatcatcc cggcagccag gacctgggaa agggggttgg
      781 ccctattctt tcctgcaatg tccggggccg aaccgtggat gggctcgaaa taggcccgtt
      841 ggtctcccat gttggcggaa gggcacatcc ccaggccgcc gatcgtagcc ccccctaggt
      901 cgctgaggat atcgccgaac atattttcgg tgaccaccac ttgaaaatgc tcggggcgca
      961 tgaccagcgc ggccgaggcg gcgtctacat acaggttttc cgcatccacg gatgggaatt
     1021 ctttggccac ctcggtaaag atctggcgga agaaatagaa gcttcgcaga acgttgctct
     1081 tatccaccag ggtgactctc ctgcgcccgt cctcgggggc ccctttggac ttttccacag
     1141 ccagcctgaa ggcaaaacga cagaccctct cgcacccttt gcgggtcatg agcagggtgt
     1201 cgctggcagc ctgttcagtg atgattccgc ggccccggga gaggtagagg ccctcggtgt
     1261 tttccctgac tatgacgtaa tcgattcttt ccgggggacg gtccttgagg gggatggcaa
     1321 ccttgggaaa aagcttaatg ggccggacgt tggcgtaaag atcgaaaccg cttcgcagca
     1381 cccctcccaa gacacctgct tctgtgccat ctggcaggcg gactccgggc agtcccaccg
     1441 gtcccttcat ggcggcatca gccttgcgga tcgcctcaag agtttcgggg gagatattga
     1501 ccccattttt ttgatagtaa ttcgctcccg cttcatggaa ttcataggag aggcggaagt
     1561 ccccggcatt ctcctggacc ttttccagaa cctcaagagt cgcatcgacg agttccggcc
     1621 caattccgtc cccccggatc accacgattt tgtaactttt caccggccca cacctcatcc
     1681 ttctcttttt tgcttcccat tttagcttaa atacatctgc ttagcaaaag atatcccttg
     1741 gaacagtctc attttagggt aaaccttgag cgggaagact ctacccacgg gattctcgct
     1801 gccaaggttc gaaattcggg attttcttga aatattccac tgtctttagt atattccttc
     1861 tttaatccgc gataaagtta tgtagcgttt ttccccatgg tcaaagattc caagatgaac
     1921 ccatctccct ttctttccct gcgaggaatc agcaaatcct tccgggcagt aaaagccaat
     1981 gatcaagtgg acctggatgt ttaccgcggg gaagtccacg ctctcctggg ggagaacggc
     2041 gcggggaaaa gcaccctcat gaaagtcctc tatggattct accatgcgga ttccggggag
     2101 gttcggctcg acggcaagac cgtccatatc cgctctcccc acgacgcccg caggctccgg
     2161 atcggaatag tcttccagga tttcgtgcag gttccggctc tgaccgtagc ggaaaacatc
     2221 gccctcttcc ttcctgatct gcctgtcgtc ctggacaagt tggatatctt gaaacgaatc
     2281 ggggagattt cccacaggta cagccttgcc gtggaccctc gggcaccggt ccggcagctc
     2341 tcagtgggag agagacagaa ggtagagatc ctaaaactta tcctggccga tgcgcaaatc
     2401 cttatcctgg atgagcccac ccgcagcctg gctcttcacg agattgaagg gctctttcgg
     2461 gtcttcgcca accttcgccg ggatggttac gcggtggtct tcatcaccca caagttgaag
     2521 gaggtcctga cctgtgcgga ccggattacg gtaatgcgcc ggggaaaggt taccggaacg
     2581 ctggccggct ctgaggcaat agagaccagg ttggtttccc tcatgttcga ggaggaggtc
     2641 cgcgaaacct cccggatcga gcgcacccgg gccccgggcg gagttgaacc gatcctgcag
     2701 ctgagaggag tccacacctc cggagagggg ggggcggtgg gcctccagga cattgatttg
     2761 gcggttctcc cgggagagat cgtcggggtt gccggggtct cgggcaatgg ccagcgggaa
     2821 ctgggcgatg tggtcctggg cctggagaga tgttccaagg gggaaaaata catcctaggg
     2881 caagaggcca cccactggtc cgtggcccgt gcccgggcca atggcctagc tttcattccc
     2941 gaggacccgc tcgggatggc agccttcccc tggctgaccg tgcaagagaa catggccatg
     3001 gccgacacgc gacggtactc ccgaaagaac ggcttttcca tggactgggt ggcggtttgc
     3061 gccgacctgg atcaatcgct tcagcgcctc ggatttacca ttccctcttt tttcgttccc
     3121 ctagccactc tttccggggg gaacgttcag cggatgatcc tggccaggga gatggcccac
     3181 aaccccaaac tgattatcgc cttttaccct acccgcggcc tggatgttcg cagcgccgtg
     3241 gccgcccgtg agttgctcat cgcctcgcgg gatgcggggg cgggggttct gctcatctcc
     3301 gaagacctgg gagagctgtt tgtcatgagt gatcggctgg tagttatctt ccggggcaga
     3361 atcgtcaaat catgcccacc gcaggagacg aacccgaacg aagtaggaca cctaatgacg
     3421 gggtcaggtc aataaaatga gggaagcgcg caaatctgca ggctcctttc cggcgcaggg
     3481 agacgggtgg ctgagtgcca tggtccgatt cggcgggctc ttcctgtttg ccctgggcgc
     3541 ctttgccctg gtgctgttgg tcttcggcaa gaaccccatc aaagcctacg tggacatctt
     3601 cgccaacacc ctgggcagcg cctacggctt ttccgaggtc ctggtaaaga tgattcccct
     3661 gaccctggcg gcggtggccg tggctttgcc ggccagaatc tggctcatta acgtgggagg
     3721 agaagggcag ttccatatcg gggccctctt ggctgcatgg ggggctttga acttcggcaa
     3781 cctgcccgcg tgggttctca tcccctggat ttccttcctg ggattcctgg gaggagggct
     3841 atggggtctg atatccggtt tcctgcgagc ccggggctgg gtcagcgaga ccatctcgac
     3901 cctcctcttg aactatgtgg ccatcctgct cctgagtttt ttcatcttcg gtccctggaa
     3961 ggacccggag agcgccaact acccgcagag cgcgcctttc gccgacgcgg ccgtcctgcc
     4021 ctcctttttc ggcacgaggg ttcatatggg cctattctat tcccttacgg ccctggtctt
     4081 ctatcatcta attctttcgc gcacacgctg ggggctggag atgcgggcca tcggggcgaa
     4141 tgccgaggcc gccagaagga acgggatttc catcggccgg tatatcatga tactcatgtt
     4201 catcggagga ggactggccg ggctggctgg aatggccgaa gtctcggcca tccacggcca
     4261 actgcggccg agcctatctc ccgggtacgg ctacatcggg ttccttatca gctggatggc
     4321 catgggcaat gccgtgggga tgatggcggc cgcatttctt ttggccataa taacggcggg
     4381 gggggatatc ctccagatga ccctccggat tcccgggtca gtggtgaaca tcctcatggc
     4441 catcattcta tttgtcatcc tggcccggaa gggaggggga agatgatcga gccgattctc
     4501 accgggcttc tcgtcgggtc catccattcc ggaaccccgc tgctttacgc caccctttct
     4561 gaggtggtcg gcgaaagggc ggggattgtt aatctgggcc tggagggggt catgctcgtg
     4621 ggagcggtag tgggattcgc aacgaccgcc ctaaccggaa atgcggtcct gggggttctg
     4681 gccgccgctc tggccggagg actgttcaac ctattcttcg gtttcctggt catcacccgg
     4741 cgggccaacc agctggccag cggcctggcg atgatgttct gcggcgtggg actcagtgcg
     4801 ctcctggggg tttcgtttgt gggaagcaag atcaaaggac tggacgaggt cggcattccc
     4861 gttctttcgg atattccttt ggccgggccc atgttcttca attatgatat attggtctat
     4921 ctggcggggc ccatcgcggt gctcgtatat tgggtgctct tcttcacccg ctggggattg
     4981 agtctcaggg ctgtggggga gaaccctacc gcggcattcg ctgccggaaa gaatccccat
     5041 ctcctgcaat accaggctct tttcctggcc gggataatgg gggggatcgc gggggcccac
     5101 ctgtccatcg ccctggcgaa aacctggcag gaatggatga cggcgggccg cggatttatc
     5161 gccgtggcgt tggtaatttt ctccaagtgg catccgctac gggccatttt cgggtcactg
     5221 ctcttcgggg gggccattgc cctgcagctt caaatgcagg cttggggagt cccgatttcg
     5281 cccttcctgc tggacatgct tccttacctt ttgagcctgg gtgttctggc tgtctgggga
     5341 ggggcccggc ggcttgcggc gccggggagt ttggggagag tgtttcaggg aaccgaatga
     5401 attgcggatt gcggattgaa taaaaaagag aagaaagggg aggggagaat gagaagagaa
     5461 agaggggcat gggtaaggcc tttagggatc ctggcgagtt tgattctggt gctgaacttc
     5521 ggtttccctg aagcggcgcc ggctcaggcg aaattgaaag tcggggtcct tcacatcggg
     5581 tccatgaccg acggcggcta caaccaggcc catgccgagg gaatcgcggt tatgaagaaa
     5641 aaccttcccg aggtggaggt gatcgaagtc gaaaatgttc ccgagaccgc ggattccgag
     5701 agggtgatgg aaagcatgat caaacagggg gccaagctca tcgtcgctgc cagtttcggc
     5761 tacctggatc ccacccttcg agtggccaag aagtacccgg atgtgaaatt tgttcacccc
     5821 gccggctaca aacgggctcc caacctgacc acctattggg ccagcacgcc ggaggccttc
     5881 tacctcatgg ggatcgctgc cggcacgacc accaagacga acaagatggg atttgtatgc
     5941 gcccttccgg tgagcttctt tctggccaat gtcaacgccc ttcacctagg ggcccggtcg
     6001 gtcaacccca aggtggaaac ccggatcgtc ttcaccggca ccttcctgga ccccgggaag
     6061 gaggccacgg ccaccaatgc tttattggac caagggtctg atgtgatggg aatgatcgtg
     6121 gattcaccca tcaccgtggt ccagacggcg gagaagcggg gcgcttactc agtcggctat
     6181 cattacctgg gagtccagaa attcgccccc aaaggctgga tcggcggcgt ggcttttacc
     6241 tggggaggac tctacacccg attcgccaag caggtcatgg acggcacctg gaagagcgaa
     6301 aacatcctgg gtagcctggc cagcgacatg atcacccttg cgcccttcgg cccagccgtt
     6361 tccccggaag ccattaaaat ggtgaatgac gtgaagaagt cttttctcga cggcaaaaag
     6421 gaagtctttg ccggacccat caaggacaac aaaggcgctg agcgggtgaa ggccggcgag
     6481 cattggtcga cggacaatct gggaaagatg gattggcttg ccgagggcat cattggtcag
     6541 accaagtgat ccatgccgag ccctgcaccc ggcagggtgt aaggcagacc cattgccgcg
     6601 aagggtggga ttcattcatt tgctgaccca gacctccaga cctcctataa gcaatgctca
     6661 cttgatcggg gcagaggccc agcgtctcct cgcgcccggc ttccgtggag aagtgctggc
     6721 tgctctatcc gataccattt atctttccag aaaagatgag gaaattctgt ggatagccgg
     6781 ggcggagtct cccccccacc gacgttgcat cctgtcctct ccttctttaa aggctagctg
     6841 ccgtggtcag agatttttct ttgatcctcc ctatcttcgt ttggatggtt ccttttggat
     6901 ggacataagc tccggagtgg aatgggagcc ctcaatcatc aggccggaga aggcaaggcc
     6961 cctcccaaga atcgaagcaa accttcgatc cctgctcggc gcccttcagt tgctggaaat
     7021 cccggatgga cttggcccat gcatctttat gctatctgct ctcctggaag atggaaacct
     7081 tccctcttac ccaccgggtt ccttgatggc tcgagccaaa gactcaactt tagaactggc
     7141 ccggaattgc ctgcaacagg acctccttcg ctgtggtatt gggggaaggg aacttgcagg
     7201 gctggggccg ggcctgaccc cttcggggga tgatttcatg ggggccctgt tctttgccgc
     7261 ccgatcttta catcaagcct acccggggga ttttccttgg gctgaggatg caatatccga
     7321 gcttctggat tgggcaaaga cccgtactca ccccgtcagc tataccattt ttcgcgattt
     7381 ggtattgggg catggacctg cagcattgca tgacttcttt gccatgctgt tgcaggaaag
     7441 agactcctgc tcagttctga acgcagccat cagcctgacg cgaatcggac actcctccgg
     7501 ttgggacatg ctcgccggag cgctgaccgg actgctgatg gtaaagagaa gcctagagaa
     7561 tagggcttag gggaaagagc atacgaggag aagcgattat agggaatttt ttttggcttt
     7621 ctgttcaaat aagcggagga attctgtatg accctgactc aatccgatat caagcagaaa
     7681 atcgaggtgg cgaatgcgga ggcggtgaag cggatccatg aggcggaccc ggttctggtg
     7741 gatgtcgctc cggcgggtga agtaatcccg gggctaaagg accggatgat cctccactcc
     7801 gggccgccgg tcacctggga aaaaatgtgc ggcgcccaga ggggagccat gatcggagtg
     7861 gctctgttcg aagggtggtc gaagaccccc gaggaagcag aaaaggtcct tcaaagcgga
     7921 gccatccaat tcgagccgaa ccatcaccac caagccgtcg ggcccatggc gggcacaatc
     7981 tcggtttcca tgcctgtctg ggtggtagag aaccgggctt tcggaaaccg ggctttctgc
     8041 cgtcaggtgg aaggacggca gcagtttggg gagtacagcg acgaagccct ggaggggctg
     8101 cggctctggc gcaatgtctg ggctccctcg ctgcggaaag ggctcctgaa acgagggggc
     8161 ctggagctca agccgctcat cgccaaggcc ctgcaaatgg gcgatgaact gcacaaccgg
     8221 ccgatggcgg ccaccagcct ttttgccaac gccatggccg ttgccatggt ggaagccgat
     8281 gtggccaagg aacccttgct ggggacgctg aaatatatta ccaaccatga gcttctatgc
     8341 ctgggattgt ccatggcggc gggtaaagct tccgccgacc cggccacggg gatcgagttt
     8401 agcacggtcg ttgtggccat ggcgcgcaac ggcacggaat ttggggtccg ggtgagcgga
     8461 ttgggcgagg aatggtttcc cgctccatcg ccccgcgtca atgggttgta tcttccggga
     8521 tactcggaaa aggacgccgg ttgcgacatg ggggattcag caattaccga gacggtgggc
     8581 tggggaggct tcgtcctggc cggagctacg ggaattctcg ccctggtggg gggaacgccg
     8641 gaagaggcta tgaagtacag ccaggacatg cgccagatta cggtatctgc cagcccgaat
     8701 tatcgaatgc ctatatttgg gttccaggga acgccggtgg ggatcgacat tcgcaaagtg
     8761 gtccagaccg gaatcactcc gatcatcgat acggccatcg cccacaagaa ccccggatac
     8821 cccattatcg gtgcgggcct cgtgcgggcg cccctggagt gtttccagaa ggcgctgaag
     8881 gcgttcgggc aaaaatatat gaagtaaggc gctttcaagt gcggaatgaa aatacaggca
     8941 atcgaatccg aagcaggagg tcccaaatcg gaaacaattt tgaaggagtg aaattcccaa
     9001 gttccaaaca cccttttgaa catttgaaac tcggatttaa ttgttttggg ggccgaaatt
     9061 cgggttatga ctatatcaaa actaccgatt cattagagga tagatggtaa ccggttgcgt
     9121 catccgtaag aaagaatacc atgactccgt ttttctaatg cgggtggcta ggagaatctc
     9181 agaacagaaa agtgttctcc aggcggcggc cctcatgggg accgagaaga acaaggtttt
     9241 actggcggat atcgggatcg ggggaaaaga gatcgaagcg gccggcccgg gcgacttgat
     9301 cgtcgccgtt aaggcagatg atggagagac tctgtcggca atgctgggaa acctggattc
     9361 atggctacaa ctttcctcgg gacccccggg agccacgccc ttttgcacgt tggaagaggc
     9421 tctccgccgc cagcctcatt ccaacctggc ggtgatctcg gttcccgggg aatacgctgc
     9481 ccgggaggcc cgccggtctt tggaacgggg gttgaatgtc ttcctcttca gcgatcacgt
     9541 tccggtcaaa gatgagctgg ccctgaaaga gtacgcccgg gagaaaggcc taatcgtcat
     9601 gggaccggac tgcgggacgg ccctcatcgg gggaaagggg atcggctttg ccaatgccgt
     9661 gcgccgcggg cccatcgggg tgatcggggc ctcggggacc ggggtccaag agttcacaac
     9721 tctggtccat cactccggct ccggaatctc ccacgccatc gggaccggaa gccgggacct
     9781 agcggatgag attggcggga tctccctcct ctcggctttg ggttctttag agtctgaacc
     9841 gcagaccaaa gtgatcgtca tcctcgccaa gcctccggga aagcgaaccc tcgctaacat
     9901 cctccccaaa atccaggcct gctccaaacc ggtggtagcc tgcttcctgg gggagacgaa
     9961 aaatttccag caggaggacc attccttcca ggtcgcccga acgcttaatg aggctgctgc
    10021 ggcagccgtg cagatcgccg ctggaagcga aaagctgccc atccttgctg aaccccaggt
    10081 ttctcggatt cgcctccaag gtgaaaaagc cgccatgaaa cccggacaga agtacattcg
    10141 gggggtcttt gccgggggga ccttttgcta tcaatcccag cagattcttc gtgacgcagg
    10201 aattcgggtt tcttccaata tccccctgga aggcaattcg gagctgccta atcctcactc
    10261 cagcctgaaa cacaccctgg tggacatggg cgccgatgaa tttacctcca gctgtcccca
    10321 ccccatgatc gactccagcc agagaagaga gcgtatcctg gctgaggcgc aggacccgga
    10381 gatcgccgtc cttttgctgg actttatcct cggattcaat gcatctgctg acccggcggg
    10441 cgaactcctc caggcaatta ctagggctaa agaagaggtt gggaaaaggg gaggattcct
    10501 atgcgtcgtt gcctcggttt gcgggacaga cgaagacttt cagggttttg ccaaacaagt
    10561 caagttgctc cagggagcgg gagccgtcgt cttccccagc aatgcccaat cagccgcatt
    10621 ttgtgcggct ctgattcaat ctctacaaga gggacctgat ggccgataag atccgtgagc
    10681 tactagacca tggaccggtg gtaataaacc tgggagtcaa ggattttgcc gaaagtctga
    10741 aagggcagga tgttgaagtt gcccatgtgg actggactcc tccggcgggg ggggataggg
    10801 agatgatcga actcctggac caactgttgt gaaaataacg gcagagagtt cgactgaagc
    10861 agagaggcgg aaaaaagcgg gggtttagga tgcaatccgc ctcaagtttt tcctaaggat
    10921 cttttggtag ggcgattggg gcatggaaaa ttgatattcc atttgggatc tgatcttttt
    10981 gacgaacttg gatacactta agataatatc gtctgcagcg ttcgggaagc tgaccggcgt
    11041 gaacgggagg aatgggttat gttgaagaag atcgatccta caaagacaga cgcctggcgg
    11101 gacctcagca atcacttttc gaagatacag ggcgttcaca tgaaagacct ctttgctgag
    11161 gacccgcgcc ggtttgaacg attctccctc cgcttcaagg aaatcctggt ggactactcc
    11221 aaaaaccgga tcaccccgga aacaataaaa ctcttgcttt ctttggccgc ggaagtggac
    11281 ctgcgggacg ccgttgaaaa gatgtttact ggggacaaga tcaacgagac ggaaagcagg
    11341 gcggttctgc atgtggcttt gagaaatcga tctaacactc ccgtttacgt ggacgggcaa
    11401 gacgtaatgc ctcaggtaaa tgcggtcctg gaaaagatga aaagcttttc tgaaaaagtg
    11461 atcttcgggg attggaaggg atttaccggc cggcgggtct cggacctggt caacatcggc
    11521 atcggggggt cggacctggg cccggtcatg gtaaccgaat gtctgaagcc ctacgcccga
    11581 caagggttat ccgtccattt cgtttccaac gtagatggaa ctcatatcgg tgagacgctg
    11641 aaaaagctga acccggaaac cactctcttc ctcatcgctt ctaaaacctt caccacccag
    11701 gagaccatga ccaatgcttt ctccgcccgg gattggtttc tggcacgggc ccaagacccc
    11761 cggcatgtgg ctcgccattt cgtagccatt tccaccaaca gggaaaaggt aaggtccttt
    11821 gggatccatc ccgacaacat gtttaccttc tgggactggg ttgggggacg ctactccctc
    11881 tggtcggcga tcgggttgtc cattgcctgc tatatcgggt ttgaaaattt tgccgaagtc
    11941 ctgcaggggg cccatgagat ggaccggcat ttccgggaaa cccctttgga gaagaatatt
    12001 ccggtaatcc tggctctgat cggagtctgg tacaataact tcttcggagc cgagaccgag
    12061 gccattctgc cctacgacca gtccatgcac cggtttccag cctatttcca acagggaaat
    12121 atggaaagca acgggaaatc caccgatcgt aatggcaaga gggtctcgca ccagacgggt
    12181 cccatcatct ggggagagcc gggaaccaac ggccagcacg ccttctacca gctgattcac
    12241 caggggacca aactgattcc cgcggatttt ttggcgccgg
//
LOCUS       DASPRR010000149         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2133788, whole genome shotgun sequence.
ACCESSION   DASPRR010000149 DASPRR010000000
VERSION     DASPRR010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133788"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VEL68_05560"
     CDS             <1..256
                     /locus_tag="VEL68_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35468.1"
                     /translation="PNPAIHTNRCYTYLVKNAAYKNPPKPDSTEDIEVLRLPLSEIPR
                     LIHDGKISHALVVAAFYWYFSIPEFKNAGTRKGRIKDGKV"
     gene            486..1745
                     /locus_tag="VEL68_05565"
     CDS             486..1745
                     /locus_tag="VEL68_05565"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ35469.1"
                     /translation="MDSEKMRAFIREKRKRVESSKLPLKDIRVIDLGAVVAAPFAATM
                     LGDFGAEVIKIEPRDVPDAIRFWSVVDGKYQPYWLFNSRNKLPITLNLKHPKGQKILR
                     QLVERADVLVENMRPGTLDRLGFPAKELWKINKGLVIGRISGYGQTGPYAAKPGFGTL
                     AESMSGFAYLNTHPGQPPTSPPLPLADMLTGLHLALGVMVALRTQKRGVEGGQEIDLS
                     LYEPLLGLLGPEFLYYSLSGNIPGPMGNEAAFAAPRNNFQTRDGRWVALSGSAQAPFE
                     RMMDLVGHSELKTKPGFRNNSERIQTESRRVLNQVIGDWIRSKTREEVIAECEKAGVT
                     IGPVYNMEEISKDVHVKERGTIRAVQEPVTGKTLLFPGNPIRMPPVSPEVQFPGLPMG
                     GAIEFVLEDLLGYSPEEVAKMRTEGVV"
     gene            1848..>2024
                     /locus_tag="VEL68_05570"
     CDS             1848..>2024
                     /locus_tag="VEL68_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXZ35470.1"
                     /translation="MPVMRSVFYVPGNNEKMVAKSPEFPADIITLDLEDSVPPAEKAK
                     AREMTRENLKHAGSG"
BASE COUNT          463 a          500 c          620 g          441 t
ORIGIN      
        1 ccccaacccg gccatccaca ccaaccgctg ttatacatat ctggttaaga atgccgccta
       61 taagaatccc ccgaaaccgg actcgaccga ggatatcgaa gtcctacgcc ttcccctttc
      121 cgagattccc cggctcatcc acgacgggaa gatttcccat gccttggtgg tggccgcttt
      181 ttactggtac ttttccattc cagaattcaa aaatgcgggg actcgcaagg gaagaattaa
      241 ggacggaaaa gtctgagaac aagccactcg gctgtccttt ataagggccg agtcgtatac
      301 tttaaaagag cttgcataaa tcatctgttt atcgtataaa ttacctctac ctttgaggga
      361 tgaaagaaaa gggagaagtt cacagactgg gaaaggactt ccctcatcca catcatatct
      421 tctaaagatc aggcttctca actcagaatt ttcccctagt ggggtgacca tggaaggaga
      481 aggctatgga ttccgagaag atgagggcat ttattcggga aaagaggaag cgggtggaga
      541 gttccaagct tccgctgaag gatatccggg tgattgacct gggagcggtt gtggcggcgc
      601 cctttgcggc cacgatgctc ggggactttg gagccgaggt gatcaagatc gagccccgcg
      661 atgtgccgga tgccattcgt ttctggagcg tggtggatgg gaagtaccag ccttactggc
      721 tgtttaattc caggaacaag cttcccatca ccctcaacct gaagcacccg aaggggcaga
      781 agattctgcg ccagttggtg gagagggcgg atgtgctggt ggagaacatg cggccgggga
      841 cattggaccg cctgggtttt cccgccaagg agctctggaa gatcaacaaa gggttggtga
      901 tcgggcgcat ctcgggctat gggcagacgg gtccctatgc ggccaagccc ggttttggca
      961 ccctggccga atcgatgagc gggtttgcct acctgaatac ccatcccggg cagccgccca
     1021 ccagccctcc tttgccgctg gcggatatgt tgaccggctt gcatctggcc ttgggggtca
     1081 tggtggcttt gcgcacgcaa aagcgtgggg tggaaggggg gcaggagatt gatctttccc
     1141 tgtacgagcc gctcttgggg cttttggggc ctgaatttct ctattattcg ttgtcgggga
     1201 atattccggg gcccatggga aacgaggcgg cttttgccgc tccgcggaac aacttccaga
     1261 cccgggacgg aaggtgggtg gcgctttcgg gcagcgccca ggcccccttc gagcgcatga
     1321 tggatctggt cggtcattcg gagctgaaga ccaagcccgg gtttcggaat aacagcgagc
     1381 ggatccagac ggagagtcgt agggtcctca accaggtcat tggcgattgg attcggtcca
     1441 agacgcgaga ggaagtcatc gccgaatgcg aaaaggctgg ggtgacgatt gggcccgttt
     1501 acaacatgga ggagatttcc aaggatgtcc atgtgaagga gcggggcacc attcgcgccg
     1561 tgcaagagcc ggtaaccggg aagactcttt tatttcctgg gaaccccatc cgcatgcctc
     1621 cggtctcgcc ggaggtccag tttcccggcc ttcccatggg tggggccatt gagtttgttc
     1681 tggaggacct gttgggctac tcccccgaag aagtggcgaa gatgagaacg gaaggagtgg
     1741 tatgagaaag aggagaacat acggaaccac agaggtcgca gagcaatact cggtgtcctc
     1801 tgtggttata ttttcacaat gcgtctaaat caaataggag gtgtgcaatg ccagtaatgc
     1861 gatcggtatt ttatgttccg ggaaataatg aaaagatggt ggccaagtct ccagagtttc
     1921 ccgccgacat cattaccctg gatctggagg attcggttcc cccagcggaa aaggcgaagg
     1981 cccgggagat gacgcgggag aacttgaagc atgcgggatc ggga
//
LOCUS       DASPRR010000150        22744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_687310, whole genome shotgun sequence.
ACCESSION   DASPRR010000150 DASPRR010000000
VERSION     DASPRR010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 22744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22744)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22744
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687310"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VEL68_05575"
     CDS             complement(<1..581)
                     /locus_tag="VEL68_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ35471.1"
                     /translation="MSQGFVEKLIKFYIPLGLFLFGLLFPFYWMFNTAFKSDAELYNL
                     KLNPFVIHEPTLHNVYTLFQETLFPQWMINTFFVSILATVISLAASLMAAYAIQRLRF
                     RTSGAMGISIFFSYLVPPTILFIPLATIIFELKLWDSLWSLVLVYPTFLIPFCTWLLM
                     GYFKSIPKEMEECAMIDGASRFRVFWQIVVPLAL"
     gene            complement(586..1431)
                     /locus_tag="VEL68_05580"
     CDS             complement(586..1431)
                     /locus_tag="VEL68_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008532635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ35472.1"
                     /translation="MGVAFVIPALILLFLFLAYPLVLGIWLGFTDTMVGRKGEFIGLA
                     NYISLLQDRLFWLVTYYTFLYTAAAVFFKLILGFILAIVLNRNFRAKGFVRAIVLLPW
                     IIPTVLSAICFWWLFDTTFSGITWVLIKAGLISHKIDYLGNMTNARISLIVANVWRGI
                     PFFTIGLLAGLQTINPTLYEAASIDGAGGWHRFRHVTVPLITPLLAVVTTFSTIWTFA
                     DFQLIWVITKGGPANATHLYGTLSFQRAIPGGQFGEGAAISAFILPVLIMCVLICYKY
                     LRREE"
     gene            complement(1545..2957)
                     /locus_tag="VEL68_05585"
     CDS             complement(1545..2957)
                     /locus_tag="VEL68_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ35473.1"
                     /translation="MEDKELKEGLTRRDFIKTGLAGAAAVGAASMAADALAQAKKPAP
                     AKTAAPAGPPSPPALKPEKGITLRVLRWSVFVKSDQEVWDKNTKAWEDKTGNKVITEY
                     ISWEDVRTKAAMTASVGAGHDIVLGWHDDPHLYPDKLIDVSDLAIYLDKKYGGWEPVC
                     YQYGINQKTKRWIALPVGAPTLCANYRVSWMKEAGFDKFPDKIPEFLKLCKALKAKNH
                     PTGFALGHAVGDGNNNWHWWLWSFGGKVVEPDGYTLCLNKKETWEALEASKEFYDTMI
                     PGVGSWLDPHNNKAFLAEEISITGNGISIYYAAKQQFPKIAEDMDHANMPIGPVGKPT
                     ELHLFNQAFIFKHCKYQQAAKHYLLYMFEQEQYGPWIEAMRGYVTPSLKDYKKLPVWT
                     IDPKHTPFRDSLSRMLWNGYAGPEGPASAATMAEYVVVDMFADVCVGGKSPKAAAAAA
                     QDRAARFYRAPAPKAPAKKT"
     gene            complement(3263..4150)
                     /locus_tag="VEL68_05590"
     CDS             complement(3263..4150)
                     /locus_tag="VEL68_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXZ35474.1"
                     /translation="MQTGTKKRKNILRELLKAGKPTLGTHVHVSWPGMVEVIGYSGGM
                     DYIEFSGEYAPYDLYALENFGRAIDLFPHMSSMIKIDQEPRTYLAGRAIGSGIQNLLF
                     ADIRSVEDTREAVAAARAETPRTKGYVGAAMRRDVGYLLTCGSPIYVDQLQDGVVALM
                     IEKESAVENLESILSVPGVDMIQFGPADYSMSIGIPGQWNHPRIREAERFVIETALKM
                     GVAPRVELGDFRDADRYLKMGVKHFCVGWDIDIIHKYCKDQGAVFAEVFGKERNEAKG
                     RRAIKVEVGYAAARKRRKG"
     gene            complement(4347..5192)
                     /locus_tag="VEL68_05595"
     CDS             complement(4347..5192)
                     /locus_tag="VEL68_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ35475.1"
                     /translation="MVGSGKFTKRALNLYLPLTAFVIFLLFPFYWMIIVSLKPTPDLF
                     DLKFNPFWIQHFTVENYLYLFQNTDFPEWIKNTIIVSLSATSISLACSVLIGYALARL
                     KFPGSNFLGVGIFLAYLVPPTLLFLPLAQVIAMLGLYNTYWALILTYPTQLIPFASWL
                     LMGYFRTIPKEIEESAMADGCTRLATLVRIILPLSVPGLLSAGIFCFTLCWNEFLYAL
                     IFMSSGPMKTIPVGTVSDLIKADTLFWGSLMASAVLGSFPVALIYSFFVKHYVSGLTA
                     GAVKG"
     gene            complement(5194..6132)
                     /locus_tag="VEL68_05600"
     CDS             complement(5194..6132)
                     /locus_tag="VEL68_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ35476.1"
                     /translation="MSVELSRAAESLPSRAGILAKAKNLLERQASLGVILMIPGVILL
                     MVFMAYPFFLGIWLSMSDSMIGRIGRFIGLRNFADLLTDSIFLQTTRNTFTYALITVP
                     FKAVLGLGLALALNNRMRFSNPMRAVVMMPWIVPTALSTLGWFMIFDPVFSPISWLLR
                     QLGVIHKNINFLGDPGLAIAMLCMVNIWRGIPFFGITILAGLQAVPQELHEAAAIDGA
                     NAWHRFRHVTIPAIKGIVLIASLLSIIWTFADFQLIYILTKGGPANQTHIFGTYAYQI
                     GLSATEIGMGAAIALYMFPILSVFAMLLLMYLRKED"
     gene            complement(6244..7587)
                     /locus_tag="VEL68_05605"
     CDS             complement(6244..7587)
                     /locus_tag="VEL68_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ35477.1"
                     /translation="MAKKKLGERKGISRRQFLKAGVAGAAAVGLTGLGGGLRRAFGQA
                     PAAIKGTRLAILQGTYFIPAAQELYKKQAQEWGKAMGVTMATDFLNWPDLQPKIAAAI
                     QAGGVDIVELWPLWNFLYKSSLLDLTDMAEEVGRRGGGYEQFVLNSGKVGGRYLGIPT
                     GQSNTAANYRISWFKEAGVADAEDGRKLDMTWDEYFAVGKKLKTQGKPFGQALGHSTG
                     DPPSYCYPYMWCHGAMEVAKDGKTVQFNKPEFVDGMRKFIQAWKDCYDETGTSWDDSN
                     NNRAFLSGQISSTFNGSSIYAVAKKQNPDIAKDMNHMLFPKGPAGRFYQLGTRTFAIL
                     KNSKNIPAAKEFLKWWFEDKQYDEWWRTQEGYHLQHVVRLAKDPMWDQDPKMGSFRDE
                     PKYGRTQGYAGEPNEKASLAWSKYIIVDTFAKAAQSGDAAGSVKWGAEQLQRIYG"
     gene            complement(7733..9076)
                     /locus_tag="VEL68_05610"
     CDS             complement(7733..9076)
                     /locus_tag="VEL68_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ35478.1"
                     /translation="MEKKKRTERKGLSRREFLKAGVAGAAAVGLAKLGTGTPPVFGQA
                     PAAIKGTKLAILQGTYFVPAGQELYKKQAQEWGQANGVTVATDFLNWPDLQPKIAAAV
                     QAGGVDIIELWPSWNHLYQASLLDLTDMAEEVGKRGGGFEEYVVNSAKVGGRYLGIPS
                     GQSNGSMAYRISMFKEVGVKDAEDGNKLDFTWEEYHAIAKKLKAKGKPFGQALGHSTG
                     DPPSFCYPYMWSNGAMEVDKDGKTILFNKPEFVDAMKRFIQAWKDGYDETGTSWDDSN
                     NNRAYHSSQISCTFNGSSIYFVAKKNFPDIAKDTSHLLIPKGPAGRFYWLGSRTFAIL
                     KNSKNVPAAKEFLKWWFQDKQYDEWWRIQEGYHLQHVKRLSKDPMWDKDPKMTPFRDE
                     PKYGRDLGYAGPPNEKASMAYSKYIVVDTFAKAVQSGDAAASVKWGAEQLQRIYG"
     gene            complement(9403..10440)
                     /gene="pdxA"
                     /locus_tag="VEL68_05615"
     CDS             complement(9403..10440)
                     /gene="pdxA"
                     /locus_tag="VEL68_05615"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXZ35479.1"
                     /translation="MMKKDKVPIAITMGDAAGIGPEIIVKALERKDLRRKALSIVIGD
                     AKTMEQALSIAKSNLKIQPISEVEQAKDDAALIPVLDLNNIDLPRLKHGKVDPMPGKA
                     AVEYIQKAVTLAMEGRVGAIVTAPIHKEAINKAGFHYAGHTELLAHLTNTKEYAMLLA
                     HGPFRVSHVTTHTSMRKACDRIKKDRVFTVIRLTHEVLKQLGIENPRIGVAGLNPHSG
                     EDGLFGDEEKTEIAPAIEEAKNMGWSVDGPVPPDTVFTKMKGKQYDAVVAMYHDQGHI
                     AVKLVGFSMKPGGKEWDKMSGINMTMGLPIIRTSVDHGVAFGKAGEGRANPQSMVDAI
                     KLAIQLAKERK"
     gene            complement(10437..11681)
                     /locus_tag="VEL68_05620"
     CDS             complement(10437..11681)
                     /locus_tag="VEL68_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HXZ35480.1"
                     /translation="MNKPEYIPQIALIADDLTGSLDTGLQFRKKGLVTIVPLNWNHPL
                     PQAQGLVLNTNSRNLPGDVAYRRVYRICRRLKVKGIYKKIDSTMRGNVGKEALAILDA
                     QKIAKAIVVPTVPVMGRAVEGGILRVHGTPLLKTAYARDPFHPLWTSRVASLLEKETA
                     EPVGFIGIHEVRKGPSYLAEKIQETSARLLSLDAVFQPDLKIIAQACALLAGQVLPCG
                     SVGLADELNLPIQQEERKLREKGPRRPLLIISASRNPTTANQIDIARNHSRFPLVEPD
                     LDRLTNARTSGPEAKAAGQRLVEVLSRGDGAILTTTFQKHLSGKETMIPKALGKAAVF
                     LLGELRLGGLVLTGGDLAMGVVERLSSSALRIEEEVLPGIPCSTLTDGPFKGLRMVTK
                     AGGFGEKDALWRIIQYLRGDHT"
     gene            11943..12452
                     /gene="tadA"
                     /locus_tag="VEL68_05625"
     CDS             11943..12452
                     /gene="tadA"
                     /locus_tag="VEL68_05625"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HXZ35481.1"
                     /translation="MDLRPLNREDAQFMRIALEEARKAGEEGEVPIGAILVSEGEVIG
                     RGRNRPIALSDPTAHAEIQALRQGGMKVGNYRLPGSTLYVTLEPCIMCAGAILQARVG
                     RLVFGNADPKGGAVSSLYSLLEDSRLNHRVEVTSGVLQAECRELLRRFFHERRKNIFT
                     TEGTKDAED"
     gene            12658..12751
                     /locus_tag="VEL68_05630"
     tRNA            12658..12751
                     /locus_tag="VEL68_05630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12693..12695,aa:Ser,seq:cga)
     gene            12859..12952
                     /locus_tag="VEL68_05635"
     tRNA            12859..12952
                     /locus_tag="VEL68_05635"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12894..12896,aa:Ser,seq:gga)
     gene            13087..13185
                     /gene="ffs"
                     /locus_tag="VEL68_05640"
     ncRNA           13087..13185
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEL68_05640"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13284..15011
                     /gene="dnaX"
                     /locus_tag="VEL68_05645"
     CDS             13284..15011
                     /gene="dnaX"
                     /locus_tag="VEL68_05645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF011526.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXZ35482.1"
                     /translation="MSYKFYEVLARKWRPQTFEEVVGQKPITQALQNAIATQRVAHAY
                     LFAGSRGVGKTSVARILAKALNCQNGPTPHPCNDCNFCREVRDGISVDVLEIDGASNR
                     GIDEIRELRENVKYLPAKNKYKVYIIDEVHMLTEPAFNALLKTLEEPPPHVIFIFATT
                     EPHKIPLTILSRCQRYDFKRITLPSLLEQLRKIASQEKLEISDPSLHLIARQAQGSMR
                     DAQSLLDQVLSFSGPKVSDQEVLEALGIIDRKVLHETIQALGEGDRLRLLQIVEEVYN
                     FGYDLKEFCGELAQLMRDLLVLKVLSNGFQEAAKLIDLPEEELRDLSRQVDKFSLEEL
                     QALFRAFLSAHDEAARSAFPRLVLEMTLTRVSRRKPVLSVEEVLDKLRAMEERLLAGG
                     VEVAPGSLRPAALAPARQGDVLAETMEEEGAEEVPEAATPQREADEEGALELTGEVKE
                     KWKDFVTFAKKKKPPFASLLEHGEPLALDDARLEIVYPENSFYLERMQEADNLTFLQD
                     LSREFFKAPMKVRVSGKKAGSLPRRGPENQGERKNSKREREEETLNHPLLREAINVFG
                     GRVVEIKNL"
     gene            15022..15336
                     /locus_tag="VEL68_05650"
     CDS             15022..15336
                     /locus_tag="VEL68_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXZ35483.1"
                     /translation="MSKGFGNIMHQAQQLHAKMVKIQEEMAVRTAEASAGGGMVTAVA
                     NGRQELVSMKIEKEVVNPEDVEMLQDLIVAAANAALKKAQEMVSAEMKKLTGGISIPG
                     LM"
     gene            15344..15946
                     /gene="recR"
                     /locus_tag="VEL68_05655"
     CDS             15344..15946
                     /gene="recR"
                     /locus_tag="VEL68_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXZ35484.1"
                     /translation="MTIQHAQPIGRLIQELSKLPGIGEKTASRLAMHILRAAKEDAQG
                     LARAILEVKEKIRFCSQCFNLTDQDPCRVCQDPKRNREILCVVSSPEDLIALEKSGGF
                     RGLYHVLHGVLSPLEGVGPKDLRLSELLSRLQGGEVKEVILATSPSVEGEATAQYLSQ
                     IIKPMNVRVTRIARGVPMGGDLQYIDEVTLSKSLENRSPL"
     gene            16020..17501
                     /gene="cysS"
                     /locus_tag="VEL68_05660"
     CDS             16020..17501
                     /gene="cysS"
                     /locus_tag="VEL68_05660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXZ35485.1"
                     /translation="MSLKIYNTLTKKKEEFVPLEEGRVKMYVCGVTVYDLCHIGHARA
                     AVVFDIIYRFLRHMGYRVTYVQNYTDVDDKIIKRANQEGVTSQVIAERYIQAHAQDMD
                     ALGMEKPTHQPKATENIPQIIHLIQKLVDKGVAYLVDGDVYFSVEKFSPYGKLSARDP
                     EEMQAGARVEVDERKKNPLDFALWKASKPGEPEWESPWGKGRPGWHIECSAMSQRYLG
                     ESFDIHGGGKDLIFPHHENEIAQSEAASGKPFVRYWVHNGFVNINQEKMSKSLGNILA
                     IRDLLLEYHPEALRLFLLSSQYRSPVDFSSQHMAEANANLDRFYSALLGIEEFLSAKG
                     GVSSPSVAGLKGAAKEVFEKVSTTRESFEEALKDDFNTALALGYLHDLTRMLNRVLVD
                     KAFRKDPAAAALLSQGRDCLLTAGEVLGLFRVKPAEYFADQRRRFLGAKGMKEEQVLN
                     LIARREAARKEKNWTLADEIRREAAALGIALEDGPKGTTWRPT"
     gene            18195..19697
                     /gene="hflX"
                     /locus_tag="VEL68_05665"
     CDS             18195..19697
                     /gene="hflX"
                     /locus_tag="VEL68_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXZ35486.1"
                     /translation="MVGGPSGVWIPDLSAFRLGSGPRLRGLRCVHTHLKGEALSHEDL
                     ADLALLRLDLMAAIGVLESSLPGDIFLANLTPINEKEESWRCWDPLPLANLEVNALQI
                     IQALEEEFRRSQRVREAGDHRDRAILVHASSAPKWAIEDSLNELADLADSAGVVVLDR
                     LSQRRHQFHPRFLMGREKLMGLSITAFQQGADLLIFDQELNPSQMRSIADLTELRVID
                     RTQLILDIFAQRAHTREGKLKVELAQMKYLLPRLTARDSGLSRLTGGIGGRGPGETKL
                     EIDRRKVRDRIHRLSQELENINRSREVRRVRREKNQIPVISIVGYTNVGKSTLLNALT
                     KSQVYVEDKLFATLDPTTRRLRFPREREAIITDTVGFLRDLPKDLMDAFRATLEELGD
                     ADLLLNVIDISHPRFEDQMAAVEKILEDLGLKQIPILRVFNKADRVDRETAQRLSRAF
                     DAVCISALRPETFPPLIEKMEIVFSLRKNGVLSPSPPLDIPMDYPVPEAV"
     gene            19872..20207
                     /locus_tag="VEL68_05670"
     CDS             19872..20207
                     /locus_tag="VEL68_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXZ35487.1"
                     /translation="MPIYEYEATSPGRRCRKCSTVFEVIQRISDSPLASCPECRGEVR
                     RRISRPRVILPGSARGQDEVEQKVADYERKGMYSHAAELADKESEKPEKAQLKERAME
                     NYKKAGYDF"
     gene            20342..22495
                     /gene="rnr"
                     /locus_tag="VEL68_05675"
     CDS             20342..22495
                     /gene="rnr"
                     /locus_tag="VEL68_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXZ35488.1"
                     /translation="MRKSSKKKHSPSSSPLKEILSAIRTRKGQPFTLTDIAQALDVEP
                     SRRRELRQALRALVDERKIMKLDRRHYGLPPKGKVVIGRVQAHRDGYGFLIPEDSSLP
                     DLFLSRREVQDLMHRDKVALHLSCKERGQKRRPRVVEVLERANRRVVGRYEEGAKWDR
                     VVPDEQRLVQPIRVSKKASGGARKDQMVLAEITRYPAGMEGPEGKVLKVLGDPDDPRL
                     DSEIIIHKYDLPDDFPPPVLKEASMVPAAVQEARAGKRRDLRWVPFVTIDGETARDFD
                     DAVAISREPGGKLKLWVSIADVSHYVQEDSALDKEAFRRGTSVYFPERALPMLPHALS
                     MGTCSLNPEEDRLCMTVALEFDWQGEVLQADFFPSLIHSHARLTYTRVKEMLQQDQHD
                     PAARPPGIYEDLRTMADMCRKLRARRMERGSIDFDLPEPEVILDVQGQIEEIVKRERH
                     IGHQIIEEFMIAANEAVARFLDQRGSPSLHRVHEAPDSKKVEEFKEFVEHLGYPFPLK
                     GKLQPRIFQRFLERVRGKPEEKSVNYLLLRSMRQARYSESKPGHFALAADHYLHFTSP
                     IRRYPDLVVHRILKEALQHGGIPERRARSWQKKLPGIAEHSSQRERVAMEAEREIVDR
                     KKVQFMRDKLGQEFNGYISGVVPFGIFVELEEFFIEGLVHLTRLPEDHYEFLEKKHTL
                     AGRRLNRLFRLGDRVRVRVEAASLERRQVDFGLVQ"
BASE COUNT         5151 a         6165 c         6524 g         4904 t
ORIGIN      
        1 agggccaggg ggacgacgat ctgccagaaa acccggaacc ggctggcccc gtcgatcatg
       61 gcgcactctt ccatctcttt gggaatagac ttgaaatatc ccatgagcag ccaggtgcag
      121 aaggggataa gaaaagtggg atagaccaag accagggacc acaggctgtc ccagagtttc
      181 agctcgaaga tgatcgtggc cagggggata aagaggatcg tcgggggtac gaggtagctg
      241 aagaagatgg aaatccccat ggcgccggag gtccgaaagc gcagccgctg gatggcatag
      301 gcggccatca ggctggcggc cagggagatc actgtggcca gtatggacac gaagaaggta
      361 ttgatcatcc actgggggaa aagggtctct tggaaaaggg tgtaaacatt atggagagta
      421 ggttcgtgga tgacaaaggg gttcagcttc aagttataca gttccgcgtc cgacttgaat
      481 gcggtgttga acatccaata gaaaggaaaa aggagaccga aaagaaacag gccgagggga
      541 atgtagaatt tgattaattt ttcgacgaac ccttgtgaca aagccctatt cctctcttcg
      601 taaatattta taacagatta aaacacacat gatcaggacg ggaaggatga aagccgaaat
      661 ggcggcccct tccccgaatt gacccccggg tatggctctc tggaaagaga gggtgccgta
      721 gaggtgagtg gcgttggccg gccctccctt ggtgatgacc cagatgagct ggaagtcggc
      781 gaaagtccag atggtggaaa aggtagtcac caccgccagc aaaggcgtga tcaggggaac
      841 ggtcacatgc cggaaccggt gccagccccc tgcgccgtcg atggaggcgg cctcataaag
      901 agtcgggttg atggtctgca gccccgccag aaggccgatg gtaaaaaagg gaattccccg
      961 ccacacgttg gccacgatca aagatatgcg ggcgttggtc atgttaccca ggtaatcgat
     1021 tttatgggat atgagtccgg ctttgatcaa cacccaggtg atcccgctga aggtggtgtc
     1081 gaagagccac cagaaacaga tggccgaaag gaccgtggga atgatccagg ggaggagaac
     1141 gatggcccgg acgaatccct tggcccgaaa attccggttg aggacgatgg ccaaaataaa
     1201 tcccaggatg agtttaaaga aaacggccgc cgccgtgtag aggaaagtgt aataggtaac
     1261 cagccaaaaa agccgatcct ggaggagact tatatagttg gccaggccga tgaactctcc
     1321 cttccgtccc accatggtgt cggtgaagcc cagccagatt cccagcacca ggggataagc
     1381 gaggaataaa aagagaagga tcagggcggg gatgacaaag gccaccccca tgaccgtctt
     1441 gtcttctaaa aggctggacc agcgccctcg ccggcccagc cccctttcaa gttggatggg
     1501 aatattctgt gtgtttccca acgtctttcc ttgccaactg ccgattaggt cttcttggcc
     1561 ggcgccttgg gcgctggtgc cctgtagaac cgggccgccc ggtcctgggc tgcggctgcg
     1621 gcggccttcg gggacttgcc gcccacgcat acgtcggcga acatgtcgac caccacatat
     1681 tcggccatgg tggcggcgga ggccggtccc tcgggtccgg cgtatccatt ccagagcatg
     1741 cgggagaggg aatcccggaa cggagtatgc ttgggatcga tggtccagac gggcagtttc
     1801 ttgtaatcct tgaggctggg ggtgacatat cccctcatgg cttcgatcca gggaccgtac
     1861 tgttcctgtt cgaacatata aaggaggtaa tgcttggctg cctgctggta tttgcagtgc
     1921 ttgaagatga aggcctgatt gaagaggtgc agctcagtgg gctttcctac cgggccgatg
     1981 ggcatgttgg cgtgatccat gtcctcggcg atcttcggga actgctgctt ggcggcatag
     2041 taaatgctga tgccgttacc ggtgatggag atttcctcgg ccaagaacgc cttgttgttg
     2101 tggggatcga gccaggaacc cacgccgggg atcatggtgt cgtagaactc ttttgacgcc
     2161 tctagggcct cccaggtttc cttcttgttc aagcagaggg tatatccatc cggttcgacc
     2221 accttcccgc cgaaggacca gagccaccag tgccagttgt tgttcccgtc gcccacggca
     2281 tggccgagag cgaagccggt aggatggttc ttggctttca gggctttgca gagtttgagg
     2341 aactcgggga tcttgtccgg gaacttgtca aacccagctt ccttcatcca actcacccgg
     2401 taattcgcgc aaagcgtggg tgcccctaca gggagggcaa tccagcgttt ggtcttctgg
     2461 ttgatcccgt actggtagca caccggctcc cacccaccgt attttttgtc caggtatatg
     2521 gccaggtcac tgacgtcgat gagtttgtcc gggtatagat gggggtcgtc atgccagccc
     2581 agaacgatgt cgtgcccggc gcccacggaa gctgtcatgg ccgccttggt gcggacatct
     2641 tcccaggaga tgtattcggt aattaccttg ttgccggtct tatcttccca agccttggtg
     2701 ttcttgtccc aaacttcctg atcgctcttg acaaagacgg accagcgcag gacccgaagg
     2761 gtgatccctt tttccggttt cagcgccggc gggctggggg gtcccgcagg agccgccgtc
     2821 tttgcggggg ccggcttttt ggcctgagcc agggcatccg cagccatgct ggccgcgccc
     2881 acggccgccg ctccggccaa ccccgtcttg atgaaatctc ttcgagtgag gccttccttt
     2941 aattccttat cttccatatt tcctcacctc cctatttttt tctattggag agcgccccaa
     3001 tagtatgact ccatctagag acgggttcac ctttccctga aactttcctt aagcaggaga
     3061 aggcagaaaa taatgcccct ccaggcattc ggtgaaagcc aagtttcccg tcctttttaa
     3121 atcggcctat ttaagcatgt ttcaattaat aaaacaagaa tattttcgga gaaaagacca
     3181 aggttatggg cgcgacgcat aaggcttcaa atcgcgggtc acaagcccaa gttgccggct
     3241 aaaagccagc catgagttga tcctacccct tcctcctttt ccgagctgcg gcataaccca
     3301 cctcaacttt gatggccctt cttccttttg cctcgttccg ctcttttcca aagacctcgg
     3361 caaaaaccgc cccctggtcc ttgcagtatt tatgaataat atcgatgtcc cagccgacac
     3421 agaagtgctt gacccccatt ttcaggtaac gatcagcgtc ccggaaatcg cccaactcca
     3481 cgcggggggc gacgcccatt ttcagagcgg tttcaatcac aaacctttca gcctccctga
     3541 tccgcggatg attccattga cccggaatgc cgatagacat ggagtaatcg gctggcccga
     3601 actggatcat gtcgaccccg ggtacggaaa ggatggattc cagattttcc actgccgatt
     3661 ccttctcgat catgagggcg accaccccgt cttggagttg atccacgtat attggagacc
     3721 cgcaggtcaa caggtacccc acgtcgcgcc tcatggctgc ccccacgtac cctttcgtcc
     3781 ggggagtttc cgcgcgggcg gcggctactg cctcccgggt gtcctccact gaacggatgt
     3841 cggcaaagag aagattctgg atgccggatc cgatggcccg cccggcaaga taggttcttg
     3901 gttcctggtc gatttttatc atggaggaca tgtgggggaa aaggtcgatg gcccttccaa
     3961 aattttcgag ggcgtagaga tcataggggg catattctcc ggaaaattca atgtagtcca
     4021 ttcccccgct atacccaatc acttcgacca tgcccggcca gctcacatgg acatgggttc
     4081 ccagcgtagg tttgccggcc ttcagtaact cccgcaaaat attttttcgt ttcttcgttc
     4141 cggtttgcat ggtttctttc tccaatccat tctgtattcg tgctggttta tccttcctac
     4201 gctgaaggcg atattggcag gcaccgaccg ggggggcgat tcatgaatcg cccccactgc
     4261 gggataatct aagggtggct tacaaggacc ctaaaacctc ctgaggggca cataggggcg
     4321 ctccttttca cccgagcctc ggattcctac cccttcaccg ctcctgccgt gagcccggat
     4381 acataatgct taacaaagaa ggaatagatt aaagcgaccg ggaaagaacc cagaacggct
     4441 gaggccatga gcgatcccca gaacagcgta tcggctttga tgaggtcgct aacggttccc
     4501 accgggatgg tcttcatggg gccggaggac atgaagatga gggcgtagag gaactcattc
     4561 cagcacaggg taaagcagaa gatgcccgcc gatagcagcc cgggtacgga caagggcagg
     4621 atgatcctca ccagggtggc cagccgggtg cagccgtcgg ccatggcgct ctcctcgatc
     4681 tccttgggga tggtgcggaa gtatcccatc agcagccagg aggcgaaggg gatgagctgg
     4741 gtgggatagg tgaggatgag ggcccagtag gtgttgtata gaccaagcat ggcgatgacc
     4801 tgggccagcg gcaggaagag cagggtggga ggcaccagat aggccaggaa gatccccacc
     4861 cccagaaagt tgcttccagg aaatttcaga cgggccaggg cataaccgat gaggacgctg
     4921 cacgccaggg atatactggt agccgatagg ctcacgatga tcgtgttctt tatccactcg
     4981 gggaagtccg tgttctgaaa gagatacagg tagttttcca cggtgaaatg ctggatccag
     5041 aaggggttga acttcagatc gaagaggtcg ggggtcggct tcaaggacac gatgatcatc
     5101 cagtagaagg gaaaaagaag gaaaataaca aaagcagtca gcggcaggta aagattcagt
     5161 gctcgtttag tgaattttcc gctaccgacc atcctaatcc tccttccgaa gatacatgag
     5221 cagcagcatg gcaaagaccg acaggatggg gaacatgtaa agggcgatgg ccgctcccat
     5281 gccgatttcc gtggccgaca ggccgatctg gtaggcgtaa gtgccgaaga tgtgggtctg
     5341 gttggccggc ccccccttag tgagaatgta gatcaactgg aaatcggcaa aggtccagat
     5401 gatcgaaagg agagaggcaa tcaggacaat ccccttgatg gctggtatgg tcacatgccg
     5461 gaaacggtgc caggcattcg cgccgtctat ggccgcggcc tcgtgcagct cctggggaac
     5521 ggcctgcagc ccggccagga tcgtgatccc gaagaaggga attccgcgcc agatgttcac
     5581 catacaaagc atggcaatgg ccagcccggg atcgcccagg aagttgatgt tcttgtggat
     5641 cacccccagt tgccggagca gccagctgat ggggctgaag accgggtcga agatcatgaa
     5701 ccacccgagg gtgctcaggg cggtgggcac gatccagggc atcatcacca ccgcccgcat
     5761 ggggttgctg aagcgcatgc ggttgttcaa ggccagcgcc aaccccagac ccaggaccgc
     5821 cttgaaaggc acggtaatga gagcataggt gaaggtattg cgcgtggtct ggaggaagat
     5881 gctgtcggtg agcaggtcgg caaagttcct caggccgatg aagcggccga tccggccgat
     5941 catgctgtcg gacatggaca gccagatccc gaggaagaag gggtaggcca tgaagaccat
     6001 gagcaggatc acgcccggga tcatcagaat gacgcctagg ctggcctgcc gctcgagcag
     6061 gttcttcgcc ttggcgagga taccggcgcg agagggaagg gattctgctg cacgggaaag
     6121 ctcaacggac atgataaagc aaagctcccc attgggaaca ggcgcgaggc tgcgcctgtt
     6181 cccttcctat ttcattcaac accggagaaa ggcagtggaa ccttccggct caggagtgct
     6241 ggatcatccg taaatgcgct gcaactgctc cgccccccac tttacggagc cggcagcatc
     6301 tccagattga gccgccttgg caaaggtgtc cacgatgatg tacttggacc aagccagcga
     6361 tgccttctcg ttgggctcgc cggcatagcc ttgggtgcgg ccatactttg gctcatcgcg
     6421 gaaggaaccc attttagggt cctgatccca catgggatcc ttggccaatc ttacgacgtg
     6481 ctgcaggtgg tagccttcct gggtccgcca ccattcatca tactgtttgt cttcaaacca
     6541 ccatttcagg aactccttgg ccgccgggat gttcttggag ttcttcagaa tggcaaaggt
     6601 ccgggtgccc agttgataaa accggcccgc cggtcccttg ggaaagagca tgtgattcat
     6661 atccttggcg atgtcggggt tctgcttttt ggccaccgca tagatactgg agccgttgaa
     6721 tgtggatgag atctgtccgg aaaggaacgc ccggttgttg ttgctgtcgt cccaggaggt
     6781 cccggtctcg tcatagcaat ccttccaggc ctggatgaac ttccgcatgc catcgacgaa
     6841 ttcgggcttg ttgaattgca ccgtcttgcc gtccttggcc acttccatgg ccccatggca
     6901 ccacatgtat ggatagcaat aactcggcgg gtcaccggtg ctatggccca gagcctggcc
     6961 aaagggcttg ccttgggtct tcaatttttt gccgacggca aaatattcat cccaggtcat
     7021 gtcaagcttc cgaccatcct ccgcatcggc cacgccggct tccttgaacc agctgatgcg
     7081 gtagttggca gccgtattcg actgaccggt ggggatcccc agatacctcc cgcccacttt
     7141 gcccgagttt aggacaaatt gctcatagcc tccgcctcgc cggccgacct cttcggccat
     7201 atccgttagg tctaaaagac tggatttgta taggaagttc cacaatggcc acagctccac
     7261 aatatccacg ccgccagcct ggatggcggc ggcaatcttg ggctgaaggt cgggccagtt
     7321 caaaaagtca gtggccatcg ttacccccat ggccttgccc cactcctgcg cctgcttttt
     7381 atagagttcc tgtgccgcag gaataaagta ggttccctgg aggatggcca gccgcgttcc
     7441 cttaatagct gcgggcgcct ggccaaacgc ccggcgtaat ccgcctccca gaccagtcag
     7501 gcctacggcc gccgccccgg ctacccctgc cttgagaaat tggcgacggc taatcccttt
     7561 tctttctcca agttttttct tcgccatctt tcttcacctc cattttttct attggaaatg
     7621 tctcccctag gagcaggcgt cacaggtcag ggaaccatag cccctctcag gaaggctcct
     7681 gagaggggct ggtgaatcaa aggtcagatc gaaaaggata gagcaatggg gattatccgt
     7741 aaatccgctg cagctgctct gccccccact ttaccgaagc ggcggcgtcg cccgactgaa
     7801 ccgctttagc aaaggtatcc accacaatgt atttcgagta ggccatcgag gccttctcat
     7861 tgggcgggcc ggcgtagccc aggtcgcgtc catacttggg ctcgtcccgg aagggggtca
     7921 tcttggggtc cttgtcccac atggggtctt tggacaaccg cttgacgtgc tgcaaatggt
     7981 agccttcctg gatgcgccac cactcgtcgt actgcttatc ctggaaccac cacttcagga
     8041 attccttggc cgcggggacg ttcttggagt tcttcagaat ggcgaaggtc cgacttccca
     8101 gccagtaaaa ccggcccgcc ggccctttgg gaatgagtag gtggctcgta tccttggcaa
     8161 tatcgggaaa gttcttcttg gccacgaagt agatgctgga gccgttgaag gtgcaggaga
     8221 tctgggacga atggtaggcc cggttattgt tgctgtcgtc ccaggaggtg ccggtttcat
     8281 cgtagccgtc tttccaggcc tgtatgaacc tcttcatggc atccacaaat tccggcttgt
     8341 tgaagaggat cgtcttgccg tccttgtcca cctccatggc tccgttagac cacatgtagg
     8401 ggtagcagaa actcggcggg tcgccggtgc tgtgacccag ggcctggccg aagggcttgc
     8461 ccttggcctt cagtttcttg gcgatggcgt ggtactcctc ccaggtgaaa tccagcttgt
     8521 tgccgtcttc ggcatccttt actccgacct ccttgaacat gctgatccgg taggccatgg
     8581 agccgttgga ctggccggag gggatcccca ggtacctccc gcccaccttg gccgagttca
     8641 ccacatactc ctcgaagccg ccgccccgct tgcccacctc ctcggccatg tcggtcaggt
     8701 ccaaaaggct ggcctggtaa agatgattcc aagacggcca taattcaatg atgtccacgc
     8761 ccccggcctg gacagccgcg gcaatctttg gctgcaggtc cggccagttc aggaagtcgg
     8821 tggccacagt tactccgttg gcctggcccc actcctgagc ttgtttcttg tagagctcct
     8881 ggccagcggg aacaaagtag gtgccctgaa ggatggcgag cttggttccc ttgatggccg
     8941 cgggcgcctg gccgaatacc ggcggggtac ctgtccccag ttttgccagg ccaacggccg
     9001 ctgccccggc tacccccgcc ttgagaaact cccggcggct gagccctttc ctttccgttc
     9061 gtttcttttt ttccataacc cctcacctcc atcgtttatc tattggagag acccccaata
     9121 gtgcatcttg gttccgggta tttcgacctt ccttagcgat accgtctaag aaaggattgg
     9181 agcaagaaag ttcattaagg cagtaaaata aagagacaaa agaagccgcc gcgcagttct
     9241 gcccgtaagc ggccccattt aagcatgttt cgattatgaa aacaagaata aaaaaaagag
     9301 gttcgaggta caaggcccga gctcagaaac caaagaaacg aggcaggaga cacgagagga
     9361 aaaaataagg ttttttcttt tctcggtcct cgaaactcga tcctatttcc tctccttggc
     9421 cagctggatc gcgagtttga tggcgtccac catggattgg gggttggccc ggccctctcc
     9481 ggcctttccg aaggccacgc cgtggtccac ggaagtgcgg atgatgggca ggcccatggt
     9541 catgttgatc ccgctcatct tgtcccactc tttgccgccc ggcttcatgg aaaaccccac
     9601 cagtttcacg gcaatgtgcc cctggtcgtg gtacatggcc accacggcgt catactgctt
     9661 gcccttcatc ttggtgaaga ccgtatcggg tgggacagga ccgtccacgc tccatcccat
     9721 attcttcgct tcctcgatgg ccggggctat ttcggttttc tcttcatccc cgaagagacc
     9781 gtcctctccg gaatggggat tcaggccggc aacgccgatc cgtggatttt cgattcccag
     9841 ctgttttagg acttcatggg tcaggcggat gacggtgaag acccggtcct ttttgatccg
     9901 gtcgcaggcc tttctcatgg aggtgtgagt ggtcacgtgg gaaacgcgga aaggaccgtg
     9961 ggccaagagc atggcgtatt ccttggtgtt ggtgaggtga gccagaagct cggtatggcc
    10021 ggcataatga aatccggcct tattgatggc ctctttgtgg atgggcgcgg tcacgatggc
    10081 tcccacccgt ccttccatgg ccagggtcac ggccttttga atatattcaa ccgccgcttt
    10141 gccgggcatg gggtccacct tgccgtgctt cagcctgggg aggtcgatat tgttcaaatc
    10201 caggacagga atcaatgccg cgtcgtcctt ggcctgctca acctccgaga tcggctgaat
    10261 ttttaaattg cttttggcga tggacagggc ctgttccatg gtcttggcgt ccccgatgac
    10321 aattgaaaga gccttccgcc tgaggtcttt tcgttccagg gcttttacaa taatttccgg
    10381 gccgatgccg gcagcatcgc ccatggtaat cgcaatggga actttatctt tcttcatcat
    10441 gtatgatccc ctcgcaagta ctgaatgatc cgccagaggg cgtctttttc gccgaacccc
    10501 ccggccttgg ttaccatacg gagtcccttg aagggcccat ccgttaacgt gctacaggga
    10561 attcccggca agacttcttc ctcaatgcgt agagccgaag aggagagccg ctcgactact
    10621 cccatggcca ggtctccccc ggtgagaaca agtcctccga gccggagttc ccccaggagg
    10681 aaaaccgccg cctttcccag ggccttgggg atcatcgttt cctttccaga cagatgcttc
    10741 tgaaaggtcg tggtgagaat cgcgccgtcc cccctcgaga gaacctccac gaggcgttgc
    10801 ccggctgctt tggcctccgg tcctgatgtc cgggcattcg ttaaacggtc caggtccggt
    10861 tccaccaggg gaaaacggga atgattcctg gcaatatcga tctgattggc agttgtcgga
    10921 ttgcggctgg cggagatgat cagaagaggc ctccggggcc ccttttccct cagcttcctc
    10981 tcttcctgct gaataggaag attcagctcg tcggccaacc ccacggaacc gcaggggagc
    11041 acctgtccgg ccaggagcgc gcaggcttgg gcaatgattt tcaaatcagg ttgaaaaacc
    11101 gcatccaggg acagaagccg ggccgaagtc tcttgaatct tttcggccag gtagctgggg
    11161 ccttttctca cctcgtggat tccgataaaa cccacgggct ccgcggtttc tttctccagc
    11221 aggctggcca ctcttgaggt ccagagagga tgaaaggggt cccgagcata ggcggtcttc
    11281 agcagcgggg tcccatgaac ccggaggatt cccccttcta ccgcccggcc catcaccggc
    11341 acggtaggaa ccacgatggc cttggcgatt ttctgggcat ctaggatagc aagggcttct
    11401 ttccccacgt tcccccgcat ggtagagtcg atttttttat aaattccctt gacttttaag
    11461 cgcctgcaga ttcgataaac cctgcggtag gcaacgtctc ccgggagatt ccggctgttg
    11521 gtattcaaca ctaggccctg ggcttgaggg agcggatggt tccagtttag ggggacgatg
    11581 gtcaccagcc cttttttccg gaattggaga cccgtgtcca gggatcccgt caagtcatcg
    11641 gcgatgagcg caatctgagg gatatattct ggtttgttca ttacgagtta agctaacaaa
    11701 aaaaaggtgc tagggcaagg gatttcaaat gcggaatggg gaattcgaaa tgcggaataa
    11761 agttcgagat ttatcaggca tttcatgccg tattcggctt tccgcattcc gcttttgtcg
    11821 ggccttgaca aaaggctgga acatgatata aagaaattga attttttcaa aaggatcgag
    11881 taatcttttc aattcctttc cgttacatac ctttcttccg gcaagattat ggagagccgg
    11941 tcatcgatct tcggccttta aacagggaag acgcgcagtt catgcgaatt gctctcgaag
    12001 aagcccggaa ggccggggaa gagggggaag tccccatcgg tgcgattctc gtatccgaag
    12061 gggaagtgat tggccgcggg cgaaaccgac ccatcgcttt atccgatcca acggcccacg
    12121 ctgagattca agctctgcgg caagggggta tgaaggtcgg aaattaccgg ctgcccggca
    12181 gcactttata cgtgaccctc gaaccctgca ttatgtgcgc gggggccatt cttcaggcga
    12241 gagtcgggcg ccttgtcttc gggaatgcag accccaaggg aggggccgtc tcctcccttt
    12301 attctttgct ggaagacagc cggctcaatc atcgggtaga agtaacttcg ggagtattgc
    12361 aggcagagtg tcgggaactt ctgcggcgct tcttccatga acgacgaaaa aacattttca
    12421 ccacagaggg cacaaaggac gcagaggatt aacttaaaag aaaaaaattg atgaactcgt
    12481 aaaaagtttc tcagcccgtc accccggcga aagccggggt ccagaacgtc ttgaaataac
    12541 tggattccgg cttttgccgg aatgacgaat tcgattgaat ttcgactttt accgaggcca
    12601 tctttatttt tttctctgtg atctctgtgc ctctgtggtg aaacatggtt tttgaacgga
    12661 gaggtaccga agtggtcgta acggggtcga ctcgaaatcg acttgggggc caaaagctct
    12721 cacgtgggtt cgaatcccac cctctccgcc aattgagcaa agagcatgga gtatagagca
    12781 tggtgttcaa aaaaacgggg tggacgctat gctctttgcc ctatgcacta tgcgatatat
    12841 agccgtaggc gggatcgcgg agagatgacc gagttggccg atggtgcacg actggaaatc
    12901 gtgtgttctg ccaaaagcgg aaccgagggt tcgaatccct ctctctccgc cagaaaattt
    12961 gctgcgcaaa ttttaccttt aaccagtaac cattaactta gaaggaagag tacaaagggc
    13021 agaaatatag gttaattgtt aaaggttacc cgttgagggt attttgccct tggcaaaatt
    13081 tatgatagcc gggtcctgcg caacgaaacg ttaccaaccc cgtcaggtcc ggaaggaagc
    13141 agcggtcgta acttctttcg tgtgccgcag gggagcctgg ctatcacccc ctaaaaagac
    13201 agagcgcata gcgcctaacg ccaatatgaa agaaaagggt tacgatatgg gctatgcgct
    13261 ctgggccaag cctgaaagcc gacatgtctt ataaattcta tgaggtttta gcccggaaat
    13321 ggcggcccca gaccttcgaa gaggtagtcg gacagaaacc gattacccag gctctgcaga
    13381 atgccattgc gacgcaacgc gtggctcacg cctatttgtt tgccggctcc cggggagtgg
    13441 gcaagacctc ggtggcccga atcctggcca aggccctgaa ctgccaaaac ggccccaccc
    13501 cccacccctg caatgactgc aatttttgca gggaggtccg ggatgggatc tcggtggacg
    13561 tcctggagat cgatggtgct tccaaccggg ggatcgatga gatccgggaa ttgcgggaga
    13621 atgtcaaata cctcccggcc aagaacaaat acaaggtcta catcatcgat gaagtccaca
    13681 tgctgacgga accggccttc aatgccctct taaagacact cgaagaacct cccccccatg
    13741 tgatctttat tttcgccacc accgagccgc ataagattcc tttgacgatc ctctctcgat
    13801 gccagaggta tgatttcaaa aggatcaccc ttccatccct tctggaacaa ttaaggaaga
    13861 tcgcttccca agaaaagttg gagatcagcg acccgagtct ccacctgatc gcccggcaag
    13921 cccaagggtc tatgcgggat gcccagagtc tcctggacca agttctttcc ttcagcggac
    13981 cgaaggtttc cgatcaagag gtcctcgagg ccctggggat catcgatcgc aaggtcctcc
    14041 acgaaaccat tcaggccttg ggtgagggag atcgccttcg tcttctccag attgtggagg
    14101 aagtttataa cttcggttat gacctgaaag aattctgcgg agagctggcc cagctcatgc
    14161 gggacctcct ggttttgaag gtcctttcca acggtttcca ggaagccgcc aaactcatcg
    14221 atctgcccga ggaagaattg cgggacctct ccaggcaagt ggataaattt tcccttgaag
    14281 aactccaggc cctgtttcgg gcgtttctct cggctcacga tgaagcggcc cggtccgcct
    14341 tcccccgact ggttttggag atgaccctga cccgggtgtc caggagaaaa ccggtcctat
    14401 cggtggaaga agtcctggat aagcttcggg ccatggaaga gcggctgctc gccggggggg
    14461 ttgaagtcgc cccgggttct ctccgtcccg ctgccctcgc acccgcccgg cagggggatg
    14521 tcttggcaga aacgatggaa gaagaagggg ccgaagaggt tccggaagcg gccactcccc
    14581 agagggaggc agacgaggaa ggagcgctgg agcttaccgg ggaggtgaaa gagaagtgga
    14641 aggattttgt caccttcgcc aagaaaaaaa agccgccctt tgcttctctg ctggagcatg
    14701 gggagcccct tgctttggac gatgcgcggc tggagatcgt ttatcccgaa aattcctttt
    14761 acctggagcg aatgcaggag gcggacaact tgacctttct ccaggacctg tcccgagagt
    14821 tttttaaagc acccatgaaa gtgagagttt ccggcaagaa agcgggttct ttgccccgca
    14881 ggggaccgga gaatcaagga gaaaggaaga attccaaacg agagagggag gaagagactt
    14941 taaaccaccc cctgctgcgg gaagcgatca atgtttttgg gggacgggtc gtagaaatca
    15001 aaaatctgtg aggagacgca tatgagcaaa gggttcggca atatcatgca ccaggcccag
    15061 cagctgcatg ccaagatggt caagatacag gaggagatgg cggttcggac tgcggaagcc
    15121 tcggcgggag ggggaatggt cactgccgtg gccaacggcc gccaggaatt ggtgtccatg
    15181 aaaattgaaa aagaggtcgt caacccggaa gatgtggaaa tgctccagga cctgattgtg
    15241 gccgcggcca atgcggccct gaaaaaggcc caggaaatgg tatcggcaga aatgaaaaag
    15301 ctcaccgggg ggatcagcat cccgggtttg atgtgagtca acgatgacca tccagcacgc
    15361 ccagccgatc ggccggttaa ttcaggagct cagcaagctt cctgggatcg gggaaaagac
    15421 cgcttcccgg ctggccatgc atatcctacg cgcagccaag gaagacgccc aggggctcgc
    15481 ccgggccatc ctcgaagtga aggagaagat ccggttctgc tcccaatgtt ttaacctcac
    15541 cgatcaggat ccctgccggg tttgtcagga cccgaagagg aaccgggaga tcctctgcgt
    15601 agtcagcagc cctgaggacc tcatagccct ggaaaagagc ggggggttcc gggggctgta
    15661 ccatgtcctc catggggttc tctcccccct ggagggggtg ggacccaaag acctgcggct
    15721 gagcgaactt ctctcccgtc tgcagggagg ggaggtgaag gaagtgattc tggcgaccag
    15781 cccgtcggtg gaaggggaag ccacggccca atacctctct cagatcatca agcccatgaa
    15841 tgtccgggtg acccgcatcg cccgcggggt tcccatggga ggagaccttc aatatattga
    15901 cgaagtgacc ctgagtaagt ctctggagaa cagaagccca ttgtaaaacg aatccagaga
    15961 taaggacggt ttttatatct gcaatatgaa acccaaaccc ctgcccttca ggaaaatcta
    16021 tgtctctcaa aatttataac accctgacta aaaagaagga agagtttgtt cccctggagg
    16081 aggggagggt gaagatgtac gtttgcggag tcacggtgta tgacctttgc catatcggcc
    16141 atgcccgggc ggccgtggtc ttcgacatca tctaccgctt cttgcgacat atgggctatc
    16201 gggtgaccta tgttcaaaac tacaccgatg tggacgacaa gatcatcaag cgggccaatc
    16261 aagaaggagt aaccagccaa gttattgccg agcgatacat ccaagcacac gctcaggaca
    16321 tggatgctct cgggatggag aaaccgacgc accagccgaa ggctacggaa aatatccccc
    16381 aaattatcca tctcatccaa aagctggtgg ataaaggggt cgcctacctg gtcgatgggg
    16441 atgtctattt ttctgtcgaa aagtttagcc cctacggtaa gctttccgca agagaccccg
    16501 aagaaatgca ggccggagcg cgggtggaag tggacgaaag gaagaagaat cccctggatt
    16561 ttgccctgtg gaaggccagc aaaccaggag agcctgaatg ggaaagccct tgggggaagg
    16621 ggagaccggg gtggcatatc gaatgttcgg ccatgagcca gcgctacctg ggggagagtt
    16681 tcgacatcca cgggggagga aaggacctca tattccctca tcacgagaat gaaattgccc
    16741 agtccgaggc ggcctccggg aagcccttcg ttcgttactg ggtccacaac gggttcgtca
    16801 acatcaacca ggagaaaatg tccaaatccc tagggaacat ccttgcgatc cgggaccttc
    16861 tcctggaata tcaccccgag gctttgcgtc tctttctcct ttccagccag taccgcagcc
    16921 cggtggactt ttcttcccag catatggccg aggccaacgc caacctggac cgtttttatt
    16981 cggccttgct gggcatcgag gagttcttgt ccgccaaggg gggagtttcg tccccgagcg
    17041 ttgccgggct caagggggcg gccaaagaag ttttcgaaaa ggtctccaca acccgggaga
    17101 gtttcgagga ggccctgaag gatgatttca atacggcact cgccttggga tatcttcacg
    17161 acctgacccg aatgctgaac cgggtgctgg tagacaaggc gtttcggaag gacccggcag
    17221 cggcggcact cctgagccag ggaagagatt gcctcctgac cgcaggagaa gtcctgggcc
    17281 tctttcgggt gaaaccggcc gaatattttg ccgatcagcg gcgaaggttc cttggggcca
    17341 aagggatgaa agaagagcag gtcctgaacc tgattgcccg gagagaggcg gcgcggaagg
    17401 aaaagaactg gaccctggct gatgaaatcc gccgggaggc ggcggcgctg ggaattgctc
    17461 tcgaagatgg gcccaaagga acgacctgga ggcccaccta aaaatttagg aatgcggatt
    17521 gtggaatcaa accggatgca cattgccgat tgcccggttt ttcttaagca gggaacagca
    17581 ggcaaaattt tgcgcatccg ttttataaac ttggggcacc taagcgcaat ttaattcttt
    17641 aactctttcc tgttaatttt tctatttaaa gggtgtataa tacgcccatg tggatctgcc
    17701 actcctgtgc aaaagaggtc aaatttcaga gtggagtggg ccggcaggaa atttgcccgc
    17761 attgccggag tgacctccac tgctgcctga actgcagcct ttacgatgaa tacgcccaca
    17821 atcaatgccg ggaacctgcc gccgaatggg tgagcgatcg ggtcaaaaac aatttctgcg
    17881 agttttttgg cttcaaggat tcatccggcg cgggccgggg ccgtcgcgag agggatgagg
    17941 ccctggcaaa actcgaagcc ctttttaaaa agaagacatc ctgaaggggg tttattaaga
    18001 tccaaaagat tttcgggaat accctgggtt taaaaccttc ccagaccaag aagatcctgg
    18061 ctctctacca ccgaagagtc ccgccccaac gggtcatcac tcctgaactg gcccgatccc
    18121 tgactgaatt atcgcgagac accaaccgtc aactggctct tttaatcgac cgggggggga
    18181 acgtccggta tgtcgtggtc ggggggcctt ccggggtctg gattcccgat ctttccgctt
    18241 ttcgcctggg cagcggaccc cgcctacggg gtctgcgttg cgttcatact cacctgaagg
    18301 gagaggccct gagccacgaa gacctcgccg atttagctct tctccggctg gaccttatgg
    18361 ctgcgatcgg ggttttggag tcaagccttc cgggagatat tttcctggcc aacctgaccc
    18421 ccatcaacga gaaggaagaa agttggcgct gttgggatcc cctccccctg gccaacttgg
    18481 aggtgaacgc cctgcagata atccaggccc tggaagagga attccgccgc tctcagaggg
    18541 tacgggaagc aggggaccat cgggatcggg ccatcctggt tcacgcctca tcggccccca
    18601 aatgggctat cgaggactct cttaatgagc tggctgacct ggcggattcc gctggcgtgg
    18661 tggtcttgga cagactcagc cagagacgcc atcaatttca tccccgtttc ctcatggggc
    18721 gggagaagct gatgggcctt tcgatcacgg cctttcagca gggggccgac ctcctcatct
    18781 tcgaccagga actgaacccc agccagatgc gctccatcgc cgaccttacc gagttgcgag
    18841 tcattgaccg gacccagctc atcctggata tctttgccca gagggcccat acccgggaag
    18901 gaaagctgaa ggtggagctg gcccagatga aatacctgct tccccggctt acggccagag
    18961 acagcggtct ttcccgtttg accggaggaa tcggagggcg cggcccggga gagaccaagc
    19021 tggaaattga ccgccggaag gtgcgagacc ggatccaccg gttgtcccag gagctggaaa
    19081 atatcaaccg cagccgggag gtccgccggg tccggaggga gaagaatcaa atcccggtga
    19141 tttccatcgt ggggtacacc aatgtgggga aatcgaccct gttgaatgcc ttgaccaaaa
    19201 gccaggttta cgtggaggat aaactttttg ccacgctcga cccaaccacc cgccgtcttc
    19261 gattcccccg agagagggaa gccatcatca ccgacacggt gggatttctc cgggacctgc
    19321 ccaaagacct gatggatgca ttccgagcaa ccttggaaga gttgggagac gccgacctcc
    19381 ttttaaatgt gatcgatatc agccatcccc ggtttgaaga tcagatggcc gcggtggaaa
    19441 agatcctgga ggacctgggt ctgaagcaga ttcccatcct ccgggtgttc aataaggcgg
    19501 accgggtcga tcgggagacc gctcagagac ttagccgtgc atttgacgcc gtctgcatct
    19561 cggccctgag gcccgagacc tttccgccgc tcatcgaaaa gatggagatc gtcttttcac
    19621 tgcggaaaaa tggagttctt tccccttctc cccccctcga tattcccatg gattatccgg
    19681 tgccggaggc ggtttaaaaa cggttgcagg ttgcgggttt caggttaaat agataaagac
    19741 aaacctgaga gcatcaccca ctcagtctga aatccgcgac tcgaaacccg aaacgagtgg
    19801 gaacgattga atccgagcta caaatcggta taataatgga gtaaaagaaa ttctgcaggg
    19861 gtttgaatta gatgccgatt tacgaatacg aagccacctc tcccggcaga aggtgccgga
    19921 aatgttcgac cgtttttgag gtcattcagc gcatctccga ttccccgctt gcgtcctgcc
    19981 cggaatgccg gggtgaggtc cggcgcagaa tttcccgccc gcgggtcatc ctgccgggtt
    20041 cggcccgcgg gcaggacgaa gtggaacaga aggtggccga ttatgagcgc aaggggatgt
    20101 acagccacgc tgcagagctg gccgataaag agagcgagaa accggagaaa gcccagctca
    20161 aagagcgggc gatggaaaac tataaaaaag cggggtatga tttttgaact ggttactggt
    20221 tgctcgttgc cggttcctgg ttaatagaaa ctggcttgag ggtacgcctg gaacgagcaa
    20281 ccaggaacca gcaacttatt aattttcccc cccccgggac ttagaaagtt gaataaaaaa
    20341 tttgcgaaaa tcatcgaaaa aaaaacattc cccttcctcc tctcccctca aagaaatcct
    20401 tagcgccatt cgaacccgga aaggccagcc cttcaccttg accgatatcg ctcaagctct
    20461 tgatgtggaa ccctctcggc ggagggaatt gcgccaggct cttcgcgccc tggttgacga
    20521 gcggaagatc atgaaactgg accgccgtca ctatggcctt cccccgaagg gaaaagttgt
    20581 catcggccgg gttcaggccc atcgggacgg ctatggattc cttattcccg aggactccag
    20641 cctgccggac cttttccttt cccggcggga ggtgcaggat ctcatgcacc gggataaggt
    20701 ggcccttcat ctctcctgca aagaacgggg gcaaaaaagg aggccgcgag tcgtcgaggt
    20761 cctggaaagg gccaaccgtc gggtagtggg tagatatgag gaaggagcga aatgggaccg
    20821 ggtcgtgccc gatgaacagc ggctggtcca gccaatccgc gtatcgaaaa aggcttccgg
    20881 gggagcgcgg aaggatcaga tggtcctggc ggaaatcacc cgctatccag cggggatgga
    20941 aggtcctgaa ggaaaggttc tgaaagtgct gggcgacccg gacgaccccc gcttggattc
    21001 agagatcatc atccataaat acgatcttcc cgacgacttt ccccctccgg tcctgaaaga
    21061 ggcctcgatg gtccccgcgg cagttcagga agcccgggca ggaaaacgga gagaccttcg
    21121 gtgggtcccc ttcgtaacca tcgatgggga aaccgcccgg gacttcgacg atgcggtggc
    21181 gatttcccgg gaacccgggg ggaagctgaa gctctgggtt tccattgccg acgtgagcca
    21241 ttatgttcag gaggattcag ccctggacaa agaggccttc cggcgcggga ccagcgtgta
    21301 ttttccggag cgggctttgc ccatgctgcc gcatgctctc tccatgggaa cctgcagcct
    21361 gaaccccgaa gaagaccggc tctgcatgac cgtcgccctg gaattcgact ggcagggaga
    21421 ggttctccag gctgattttt tcccatcctt aatccacagc catgctcgcc taacttatac
    21481 ccgggtcaaa gaaatgctgc agcaggatca gcatgacccg gcggcccgcc ccccgggcat
    21541 atacgaagac ctccggacca tggctgacat gtgccggaag ctccgggcca ggcgaatgga
    21601 aagggggtct atcgatttcg accttcccga gccggaagtg atcctggatg ttcaggggca
    21661 gatcgaagag attgtcaaga gggagaggca catcggccac cagattatcg aagagttcat
    21721 gatcgccgcc aatgaggccg tggcccgctt cttggaccaa agggggtccc cttcgctgca
    21781 ccgggtccat gaggcaccgg actccaagaa ggtggaagaa ttcaaggaat tcgttgagca
    21841 cctgggctac ccgtttcccc tgaagggaaa actccagccc aggattttcc agaggtttct
    21901 ggagagggtg cggggaaagc cggaagagaa gagcgtaaac tacctgctcc ttcgttccat
    21961 gcgccaggcc cgctattcgg aatccaaacc ggggcatttc gccctggccg cggaccatta
    22021 cttgcacttc acttccccca tccggcgcta cccggatttg gtggtccacc ggattctaaa
    22081 agaggctctt cagcacggag ggataccgga aagaagagcc cgcagttggc agaagaagct
    22141 tccgggcatt gccgaacact cttcccagag ggagcgggtg gccatggagg ccgaacggga
    22201 gatcgtggac cgcaaaaagg tccagttcat gcgggataaa ctgggccagg agttcaacgg
    22261 gtacatttcg ggagttgtcc cctttggaat ctttgtggag ttggaagagt tcttcattga
    22321 aggactggtt cacctgaccc ggctccctga ggaccactat gaattcctgg agaaaaagca
    22381 taccctggcc ggaaggagac tgaaccggct gttccggttg ggggaccggg tgcgcgtgcg
    22441 cgtagaggcc gccagcctgg agcgccgcca ggttgatttc ggcctggtcc aatgataaaa
    22501 aatatttatc cgcagattac gcagatttcg cagagagcgc gaaggcatca attttagctt
    22561 cagactcttt aaatctcgtt aaatctatgg gaatctgaat tggattacat ctcgactttt
    22621 ttacttaagg cacttgaggc acctaagtgc acttaagcac acttttagtt ggtcacggct
    22681 ctcctgcgaa aattaaatta tggaagtcat ctggaaagcg aaagatttcg ggcggtcctt
    22741 caaa
//
LOCUS       DASPRR010000151        12340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1327428, whole genome shotgun sequence.
ACCESSION   DASPRR010000151 DASPRR010000000
VERSION     DASPRR010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327428"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VEL68_05680"
     CDS             <1..1151
                     /locus_tag="VEL68_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35489.1"
                     /translation="SVGAQDEVVVGIPLAITGAQAKFGEQHKNGYTLALQEILAQGGI
                     RKGALQGKKLRFHFEDDEGKPEKGKAVTEKLITRDKRPIIMGGYASSEVYAIAGTTAQ
                     YGTPFLSPSGAADDITQKGWKNVFRLNQPASEYCTGLQDFMLKVVKPTSMAIMFENTL
                     FGTSTAKGMQAWCKENNIKVLMFEPYEAGTADFKPLLTRAKASSPEVIFMVSYIMDAV
                     LLTRQTAELDIDSKLFAGGAAGFALPEFLAGVGKLAEGVMTAVLWSHDVKYPGAGKFY
                     EGFKKKFNLAPTYHGAEAYSAAYVLQDILERTNSLSKEDLYKAFGGTDMMTVFGPVKF
                     VNYKKFTNQNRVPSLVVQVINGKHETIWPPDAAAAKYVYPQPKWKDKR"
     gene            1247..2131
                     /locus_tag="VEL68_05685"
     CDS             1247..2131
                     /locus_tag="VEL68_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35490.1"
                     /translation="MSETTMFLQTMISGILIGGLYALIGVGFTLIFGVMLILNLAHGD
                     LMMVGMYVTFWLFTLYGIDPYLSLLLSMPVLFVIGILIQKYTITPVMRVQTLLPHTQV
                     LLTFAIGLVLSNITLLLFTGDYRTIKTSYSSSVWYVWGLSINVPLTIAFIIAAGITAF
                     LYLFLMKTDTGKAIRATAQDKHAAQLMGIKTERITVLTYGLGAALAGAAGSLFLPVYY
                     LFPNVGKPFTTKAFIVIVLGGMGSTIGATVGGVVLGIAESLGAVYISPGYKEAVGFVI
                     FVLVLLFLPGGLKRLTGV"
     gene            2144..3076
                     /locus_tag="VEL68_05690"
     CDS             2144..3076
                     /locus_tag="VEL68_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35491.1"
                     /translation="MDPKKQLQKYLPFIIVGLLALVPFVMDNPYFLHMIIMVFIWICL
                     GQSWNLLGGFTGQVSFGHAAFYGVGAYAGGLLAFHFEASTWWGMILGPLMAMVFAVPM
                     GLICFRLRGAYFALAMLALAELLRLIATNWIDFTNGSVGILIVPTMGKVTFYYIALGL
                     AALGFVVIRAVVNSKLGFYFIAIREDQDAAESMGIDTTKYKLISLLISSFFTGLTGGF
                     YMNYMAYIDPSVVFALTDVSIMMILVVMLGGAGTFYGPAIGSVIMVICSEAFRVYFGS
                     ANLLIMGILIVLIIIFIPNGLMDIQEYLKRRKHG"
     gene            3069..3782
                     /locus_tag="VEL68_05695"
     CDS             3069..3782
                     /locus_tag="VEL68_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35492.1"
                     /translation="MVEVKNLTKYFGGLAAVRDLSFRVEKGQIFGLIGPNGAGKTTVF
                     NMINGFYPPSSGEVLYQGKPITGLKPNVVCKKGIARTFQVVKPLSRMTVLENVMASAF
                     CRVNSIEEAREEAFKALEFCDLTHRKDFLAKSLTIGDRKRLEITRAMATKPIVLLLDE
                     TAAGLNPTELDQAIALIRKIRDQGVTILIVEHIMKVIMSISDRIHAINYGQTIAEGTP
                     QEVANNKAVIEAYLGEAYA"
     gene            3775..4479
                     /locus_tag="VEL68_05700"
     CDS             3775..4479
                     /locus_tag="VEL68_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35493.1"
                     /translation="MLKVNQIEVFYGDVQVLWGVSFKVEEKELAALVGANGAGKSTTL
                     NTISSVIHPKAGAIEFKGQPINGLEASQVVDLGITQVPEGRRLFPEMSVQENLELGSY
                     KPEAKLKRKETMEWVFSIFPRLKERKKQLAGTLSGGEQQMVAVGRGMMSRPRLLMLDE
                     PSLGLAPLMVREVFNVVERVNSEGVTVLLVEQNVKHTLSIATRAYVLENGRISMEGKG
                     KDLLNNEHVRKAYLGI"
     gene            complement(4576..4803)
                     /locus_tag="VEL68_05705"
     CDS             complement(4576..4803)
                     /locus_tag="VEL68_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35494.1"
                     /translation="MARFKEKVNCTNPACGQSAEIEFTDATRKLRFTCSSCGQLNQVE
                     VILPSDRGGAADRYGLPENGAKIKIIVLPGD"
     gene            complement(4805..4951)
                     /locus_tag="VEL68_05710"
     CDS             complement(4805..4951)
                     /locus_tag="VEL68_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35495.1"
                     /translation="MEVFRSLLQYVKENILYNPIMWGIAAVALWMIGAAFLKAWRGKD
                     EDEE"
     gene            5150..6145
                     /locus_tag="VEL68_05715"
     CDS             5150..6145
                     /locus_tag="VEL68_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ35496.1"
                     /translation="MEMKFKPEDLNFHREEYQKITAAMRSKDYPPLMKKEVKGRVNLR
                     HRRSNPYIRDGKALVSFVMAAQDLKKDIRKAVDLLGGFGKAIRSQDRVLLKPNFNSDD
                     PPPGSTAMDFLMAVIEILREHGCTRIAVGDSSGRPWVPTANVFKRLGLFERMKGAQIS
                     LLDFDQTQFIDVPIGGEYLEVIAYPKDLEDFDKIIYLPTMKTHYLAGFSLSLKLTVGL
                     AHLADRSLLHADNNQFVSQRAAEISIPVKPDLILMDGRVSFVSGGPAHGLAVHPGVIL
                     ASGDPVALDVQGVRLLQNYAAVNHLGMDAWSLPQIKTAVKHGLGIGNDDQMILIR"
     gene            complement(6210..6941)
                     /locus_tag="VEL68_05720"
     CDS             complement(6210..6941)
                     /locus_tag="VEL68_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXZ35497.1"
                     /translation="MANEKNTWKWVLGYVILILGLCILLYYYREAYLQVRQAIRFFAK
                     RESLLAYVASFGPYAPIVFIGLQILQVLVAPIPGELTGFVGGYFFGVGEGFLFSTVGL
                     TIGSWLAFLISRRFGLPFVRRFVEKETMDKFDYLMEHKGAFFSFIFFLIPGLPKDYFC
                     YLLGLSPMHVMTFLVISTVGRIPGTLVLTLQGQAVRSEEYRVFFVVLGLALVALVLTL
                     IYRDQIEKWLHKKHPPKKKPDEGRA"
     gene            complement(7105..7539)
                     /locus_tag="VEL68_05725"
     CDS             complement(7105..7539)
                     /locus_tag="VEL68_05725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXZ35498.1"
                     /translation="MPIKESYERALRRAVESAPYFQLLQIRLEQIDTGFTRFRMPFRK
                     ELTQAYGVVHGGAIASLADTAVAFALMTIIQPGERVTTAEFKINFFAAVTDGEMIGEA
                     RVVHAGKRLVSADMEVKSQDGKLIAKGIATYAIIGAPKRKED"
     gene            complement(7603..8451)
                     /locus_tag="VEL68_05730"
     CDS             complement(7603..8451)
                     /locus_tag="VEL68_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXZ35499.1"
                     /translation="MEIISLQCVALRELFGKIHGIMKIELISWKSDGLTIRGEIHLPS
                     GPTGPFPALTLCHGIPAKVKGPDDRGYPLLAERFCDKGFFVLIFNFRGAGQSEGDFDI
                     LGWARDLEKGLNTLYLRPEVDRKRVFFMGFSGGAAVSIYVAALHKEVAAVVSCASPAE
                     FRDLSTVKALEDFLTHARDVGIVKNPDFPPSMEMWKKSFKRVRPIDWIDRIPPRPLLL
                     VHGTRDDVVPVRQAHRLYEKVKGKAELFIIEGAGHRLRVEEKAMEKASEWLENIAFSN
                     QRSAFS"
     gene            complement(8525..9289)
                     /locus_tag="VEL68_05735"
     CDS             complement(8525..9289)
                     /locus_tag="VEL68_05735"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HXZ35500.1"
                     /translation="MEELIRKVTDLIRSSRKVIVFTGAGVSTESGIPDFRSPGGVWQK
                     YNPEDFYYQKFISSEESREKYWQMSREFYEPLKNAQPNAAHQAVAELEKMGKLDCVIT
                     QNIDNLHQRAGSSPQKVIELHGTAVSVSCLSCRKKYPREEVQSWLLRGVKVPRCDACG
                     GILKPDTVSFGQSMPAKETEEAFHRARNCDLFIVIGSSLVVQPAASVPLEAKEAGAKL
                     IIINRDPTYHDSYADVVIHGSAGEVMSQVLKYLKMS"
     gene            complement(9405..10589)
                     /locus_tag="VEL68_05740"
     CDS             complement(9405..10589)
                     /locus_tag="VEL68_05740"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXZ35501.1"
                     /translation="MSIRDRVAIVGVGATKFGENFEMTYQDMALEAVYEAYQDAGLGT
                     KDMEAAWLGTLSPGLTGLEGDAGASLSEPLNFYPRPVTRVSAYCCSGMEAIRNAAFGV
                     ASGEYKMVLAVGVEKMREVPPRGSLVARHIHETHPVIAKGRTAPGIFAPIATRYFHQY
                     GYGKQTLAKVAVKNHYNGSLNPKAHFRSPINEETVLKAPMVVDPLGLFDCCPTTDGAA
                     AVILTTPENAKDMKKDFILIKGMGLAITYGYFSMAFQEDNDFLGFKSTREASAMAYRQ
                     AGIKDPRKEINVAEVHDCFTITELVNYEDLGFCGRGEGGKLIEEGITALNGELPVNTS
                     GGLKSCGHPVGATGVRMVADVVNQMRGRAGKRQVKKADFGLTHTLGGPGSIACVFVLG
                     RP"
     gene            complement(10661..12067)
                     /locus_tag="VEL68_05745"
     CDS             complement(10661..12067)
                     /locus_tag="VEL68_05745"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXZ35502.1"
                     /translation="MAGISGIGVYIPKYRLGRDVMARAWGPRFISGERAVANHDEDSL
                     TMAAEAALNSLAGTDPKTVDGLIFASTTSPYLEKQASTLVATALDFPVEIYTADHLSS
                     IRAGTAGMKAALDAVKGGSARNLLVTAADTRRGEPGSDSEQLFGDGAAAVLIGSSGVA
                     ASIEGVYSLSEEFMDTWRKQEDDFVQKGDAAFVQAYGYARMVPACIQGVLKKYEMKIS
                     DVSQFVIPAPDSRVYARLTKSLNLPAGSFAEDPLLTTVGDTGAAAPLMLLAAALEKAK
                     PGERVLLCSYGAGNADAFLLQVTPEIERLKARRGVSYYQASKKSLANYEKFLKFQNTL
                     PNEPLEPYSSWALLWKEKKQNLQLYGTRCKKCGTFAFPRRRVCLNCYAKDEYEDAKLP
                     RKGKIYTFAKDHLFPNPDPPTVMAVADMEGGGRFYGQVTDCDPGQIKIGMPVELTLRR
                     FHQGDRFYNYFWKLRPLE"
BASE COUNT         2769 a         3424 c         3343 g         2804 t
ORIGIN      
        1 agtctgtcgg agcccaggat gaggtggtcg ttggtatccc cctggccatc acgggcgctc
       61 aggcgaaatt tggggaacaa cataaaaatg ggtatacgct ggccctgcaa gaaatcttgg
      121 cccagggagg aatccgcaag ggggccctcc aggggaaaaa attaaggttc catttcgagg
      181 atgatgaggg aaagccggaa aaaggcaagg ccgtgaccga gaagttaatc acccgcgata
      241 agcgtcccat tataatgggt ggatatgcca gttcagaggt ctacgccatc gccgggacca
      301 cagcccaata cggtacccct ttcctgtccc ccagcggagc agcggatgat attacccaga
      361 aaggttggaa gaacgtcttc cgcctgaacc agcctgccag cgaatactgc accggcctcc
      421 aggatttcat gctcaaggtt gtgaaaccca cgagtatggc gatcatgttc gaaaataccc
      481 ttttcggcac tagcacggcc aagggcatgc aggcatggtg caaagaaaat aatatcaaag
      541 tcctgatgtt cgagccctac gaggccggga ccgcggactt caaacctctc ctgacccggg
      601 ccaaggcctc cagccccgag gtgatcttca tggtttctta cattatggat gcggtccttc
      661 tcactcggca gaccgccgag ctggacattg actccaaact cttcgccgga ggagccgccg
      721 gctttgccct gcccgaattt ctggccggcg tagggaagct cgccgaggga gtcatgaccg
      781 cagtcctttg gagccatgac gtgaaatacc cgggggccgg gaagttttat gaaggtttta
      841 aaaagaaatt caatctggcc cctacctatc acggggcgga ggcttactcg gcagcctatg
      901 tcctccagga catcttggaa cggacgaatt ccctttctaa agaagacctg tacaaggcct
      961 ttggcggaac cgacatgatg accgtcttcg gcccggtgaa gttcgtcaat tataagaaat
     1021 tcaccaacca gaacagggtt ccctccttgg tggtccaggt catcaacgga aaacacgaga
     1081 ccatctggcc tccggatgct gctgccgcca aatacgtcta ccctcaaccg aaatggaaag
     1141 ataaacgctg agcagcgaag aagtcggtgg aagcggattc cccgggggct ttcccagccc
     1201 cggggatcga gggatatccc tgaacctttc ctgaagtgag gtctccatgt ccgaaacgac
     1261 catgtttctt cagactatga tcagcggtat tctgatcgga gggctctatg cgctgatcgg
     1321 ggtggggttc accctgatct tcggcgtcat gctgatcctc aacctggccc acggggacct
     1381 gatgatggtc ggcatgtacg taaccttttg gctctttacc ctctatggga ttgaccctta
     1441 cctgtcgctc ctcctctcca tgccggtgct ttttgtgatc ggcattctca tccagaagta
     1501 caccataacc ccggtcatgc gggttcaaac ccttctcccc catacccagg tccttctgac
     1561 tttcgccatc ggcctggtcc tgagcaatat tactctcctc ctctttaccg gagactaccg
     1621 gacgatcaag accagttaca gcagctcggt ctggtatgtg tgggggttat cgatcaacgt
     1681 gcccttgacc attgccttca tcatcgctgc cggcataacg gccttcctct acctcttcct
     1741 gatgaagacc gacacgggaa aggccatccg ggctacggcc caggacaagc atgccgccca
     1801 gttgatgggg attaaaaccg aaaggattac ggttttaacc tacggcctcg gcgcggccct
     1861 ggccggcgcg gctggttcgc tctttctccc ggtctattat ctattcccga acgtgggtaa
     1921 acccttcacg acgaaggcct tcatcgtcat cgtgctggga gggatgggga gcactatcgg
     1981 ggccacagtc gggggagtgg tgctgggaat tgcggaatcc ctgggggccg tctatatctc
     2041 ccccggctat aaggaggctg tcgggtttgt tatctttgtc ctggtcttgc tcttccttcc
     2101 cggcgggctg aagcgattaa ccggcgtata gagaggaacc gcaatggatc ccaaaaagca
     2161 acttcagaaa tatctccctt tcatcatcgt aggtctgctg gccctcgtac ctttcgtgat
     2221 ggataacccc tactttctcc acatgatcat catggtcttc atttggatct gcctgggcca
     2281 gagctggaac cttctgggag gattcacggg ccaggtatct ttcggccatg ccgcctttta
     2341 cggagtggga gcgtatgccg gcgggctcct ggccttccac tttgaagctt ctacctggtg
     2401 ggggatgatt ttaggccccc tgatggccat ggtctttgcc gtccccatgg gcctgatctg
     2461 tttccgcctc cgcggagctt acttcgccct ggccatgctg gcattggccg agcttctacg
     2521 cctcatcgcc accaactgga tcgatttcac caacggatcg gtgggaattc tgatcgtacc
     2581 caccatgggg aaggtcactt tttactatat cgccctgggc ctggcggctc tggggtttgt
     2641 ggtcattcgc gcggtggtga attccaagct ggggttttac ttcatcgcca tccgggaaga
     2701 ccaggatgcc gccgaatcca tgggcatcga cacaaccaaa tacaagctca tctccctgct
     2761 gatctcctcc ttcttcaccg ggctgaccgg cggtttctac atgaattaca tggcctacat
     2821 cgacccaagc gtggtcttcg ccttaaccga tgtgtccatc atgatgatcc tggtggtcat
     2881 gctgggaggg gcgggaactt tctacggtcc ggcgatcggg tcggtgatca tggtgatctg
     2941 cagcgaggct ttccgggtct acttcgggag cgctaacctt ttgattatgg gaatcctcat
     3001 cgtcctcatc atcatcttca ttcccaacgg cctcatggac atccaggagt acctgaagag
     3061 gagaaaacat ggttgaggta aaaaatctta cgaaatactt cggggggctg gcggcggtcc
     3121 gggatctttc cttccgagtg gagaaaggac agatcttcgg tctcatcggc cctaacggag
     3181 cgggtaagac cacggtcttc aatatgatca acggcttcta ccccccctcc tccggagaag
     3241 tcctttatca aggaaagccc atcaccggcc tcaagcccaa cgtggtctgc aagaagggga
     3301 tcgcgcgaac tttccaggtg gtcaagcccc tctcccgcat gaccgtcctg gagaacgtca
     3361 tggcctcggc cttctgccgg gtgaacagca tcgaagaggc cagggaagag gccttcaagg
     3421 ccctggaatt ctgcgacttg acccatcgga aggattttct ggccaaaagc ctgaccatcg
     3481 gcgaccgcaa gcgtttggag atcacccggg ccatggccac gaagccaatt gtccttttgt
     3541 tggacgaaac cgcagccggc ctgaacccca ccgagctgga ccaggccatt gccctgatcc
     3601 gaaagatccg ggaccagggg gtcaccattc tgatcgtgga acacatcatg aaggtcatta
     3661 tgtccatctc cgaccggatc cacgctatta actatggcca gaccattgcc gaagggacgc
     3721 cccaagaagt ggccaataac aaagcggtca tcgaagccta tctgggggaa gcctatgctt
     3781 aaggtcaatc aaattgaagt cttctacggc gacgtacagg ttctctgggg agtttcgttc
     3841 aaggtagagg aaaaagaatt ggccgctctc gtgggagcca acggggcggg caaatccact
     3901 accctgaaca cgatttcttc ggttatccac cccaaggcag gggccattga gtttaaaggg
     3961 cagcccatta acggcctcga agcctcccaa gtcgtggacc tggggattac ccaggttccc
     4021 gaggggcgca ggttgtttcc ggaaatgagc gttcaggaga acctggaact gggctcctac
     4081 aaaccggagg ccaagctgaa acgcaaagaa acgatggaat gggtcttcag catctttccc
     4141 cgcctcaagg agcggaagaa gcagttggcc ggaaccctca gcggggggga gcaacagatg
     4201 gtcgccgtag gacgggggat gatgtcccgc cccagactac tcatgctgga tgaaccttcc
     4261 cttggtctgg ccccgctcat ggtgcgggaa gtattcaatg tggtggaacg ggttaattcc
     4321 gaaggggtta ccgtccttct ggtggagcaa aatgtgaaac ataccctatc catagccact
     4381 cgggcatacg tacttgaaaa cgggcgcatt tccatggagg gaaagggaaa ggatcttttg
     4441 aacaacgaac acgtaaggaa agcctatctg gggatataaa agcaggagtg aatgcagagc
     4501 gatgaggcct gaataacgaa ggccggacct attattaacc cttaattctc agcagtcatt
     4561 actcatcact gttctttagt ctcccggcag gacgatgatc tttatcttgg ccccgttctc
     4621 tggcaggccg tatcggtccg cagcccctcc ccggtccgac ggaaggatga cttccacctg
     4681 gttgagctgg ccgcaggacg agcaagtgaa acgcaatttc cgtgtggcat cggtgaattc
     4741 gatttccgcg gactggccgc aagccgggtt ggtgcaattc accttttcct tgaaacgggc
     4801 catcctattc ctcgtcctcg tcttttcccc gccaggcctt gaggaaggcc gccccgatca
     4861 tccataaggc aaccgcggca attccccaca tgatgggatt gtagaggatg ttttccttta
     4921 catactgaag aagggaccgg aagacctcca ttgaatggta ttttctctca ttccgaaaaa
     4981 attgcaagga aaaattgaat tctacccccg gtattctctg ggccctggat agcaacctat
     5041 gaatttgatc ttttggagga atgggtgttg atcttatgcg gggtcatggg ataataagaa
     5101 ataattatcg acgtttcgga gccggagatt tttgccagga ggaaaaacca tggaaatgaa
     5161 gtttaagccg gaggacctca attttcaccg ggaagagtac cagaagataa cggcggcgat
     5221 gcgttccaaa gactatcctc ccctaatgaa aaaggaagtc aagggacggg tgaacctccg
     5281 ccatcgccgt tccaatccct atatccggga tgggaaagcc ctggtctcat ttgtcatggc
     5341 cgcccaagac ctcaagaaag atatccgaaa ggcagttgac cttctgggag gattcggaaa
     5401 agccatccga tcccaggacc gggtgctttt gaaacctaac ttcaacagcg acgacccgcc
     5461 tcccgggtcc acggcaatgg acttccttat ggcggtgatc gaaatcctgc gcgagcatgg
     5521 ttgtacgcgc atcgccgtcg gggacagctc cgggcgcccc tgggttccca ccgccaatgt
     5581 cttcaaaagg ttaggcttat tcgaacgaat gaagggggcc caaatttccc tcctcgactt
     5641 tgaccaaacc caattcatcg atgtgcccat cgggggggaa tacctggagg tcatcgccta
     5701 cccgaaggac ctggaggatt ttgataaaat catctacctt cccaccatga agactcacta
     5761 cttggccggc ttctccttga gcttgaagct gaccgtggga ctcgcccacc tggccgaccg
     5821 gtcgctgctt cacgcggata acaaccagtt cgtctcccag cgggctgcgg aaataagcat
     5881 tcccgtcaag cccgatttga tcctcatgga cggaagggtc tcctttgtca gcgggggacc
     5941 ggcccacgga ttggcggttc accccggcgt tatcctggct tccggcgatc cagtggcttt
     6001 ggatgttcag ggagtccgtc ttcttcagaa ctatgctgcc gtcaaccacc tgggcatgga
     6061 cgcttggtcc ctgccccaga tcaaaacagc ggtcaaacat gggttgggga tcggaaacga
     6121 tgaccagatg attttaattc ggtgaaaccg ctgaggtgaa agaggaaaat tagaaaaagc
     6181 ggataaaccg gatgagagtt cggggttttt caggctcttc cttcgtcggg cttctttttg
     6241 ggaggatgtt tcttatgcag ccatttttcg atctgatcgc gatagatcag ggttaaaacg
     6301 agggccacaa gagctagacc cagaacaacg aaaaagaccc ggtactcttc ggatcgaacc
     6361 gcctggccct gaagggttaa gacgagagtt cctggaatcc ttccgaccgt ggagatgacc
     6421 agaaatgtca tgacatgcat ggggctcaac cccaggaggt agcagaaata atccttgggc
     6481 aagccaggga taagaaagaa gatgaaagaa aagaaggccc ccttgtgttc catgaggtaa
     6541 tcaaatttat ccatcgtctc tttttccacg aatcggcgga caaaaggcag gccaaatcgg
     6601 cgggagatga gaaaagcaag ccatgagcct atggtaaggc ccacggtaga aaaaagaaac
     6661 ccttccccca ctccaaaaaa gtacccccca acaaaaccag tgagctcgcc cgggatgggg
     6721 gccactaaga cctgcaggat ctgcaatcca atgaagacga tcggggcata cgggccgaag
     6781 gaggcgacat aggcaagaag actttctctc ttggcaaaaa atcgtatggc ttgccgaact
     6841 tggagatagg cttcccggta ataatacagg agaatgcaca ggcccaaaat caggatgaca
     6901 tatcccagga cccatttcca ggtatttttt tcgtttgcca taaagcctga tgagtttcca
     6961 gcttttctac ttttaccaaa gaattcaggg ggaatcaaga aatatccgat gaacttatcg
     7021 acaggaaatt ggaaaaatag cagagaatat tccccgaaag aatctctcaa ggaatgatta
     7081 tttgggcaaa gtagtctcct tcgctcaatc ctccttcctt ttcggagcgc cgataatggc
     7141 gtaagtcgca atccccttgg ctatgagttt tccatcctga cttttaactt ccatatcagc
     7201 tgaaaccagg cgtttccccg catggacgac ccgggcttcg ccgatcattt ctccatcggt
     7261 cactgcggca aagaagttga ttttgaattc cgccgtggtc acccgttccc cgggctgaat
     7321 gatcgtcatc agggcaaaag ccaccgccgt gtcggccagg gacgctatgg cccctccgtg
     7381 gaccactcca taagcctggg tcagttcctt gcgaaagggc atccggaatc tcgtaaatcc
     7441 ggtgtcgatc tgttccagac gaatttggag aagttggaaa tagggagcgg actccacagc
     7501 ccgacggagg gctctctcgt agctttcttt tatgggcata ggggctccag aataagcgtt
     7561 cacctgtcag ccttcagcgg tcaagaaaca gccaaaggcg tgtcagctga aagctgaccg
     7621 ctgattgctg aaagctatat tttccagcca ttctgaggct ttctccatgg ccttttcctc
     7681 gacacgcaag cggtgtccgg ctccttcaat gataaaaagc tccgcctttc ctttcacttt
     7741 ctcgtagagc cggtgggctt gacggaccgg gaccacgtca tcccgggttc cgtggaccag
     7801 gaggaggggc cgggggggaa tccgatcgat ccagtcgatg ggcctcaccc ttttgaaact
     7861 cttcttccac atttccatgg acggaggaaa gtccgggttc ttgacaattc ccacgtcccg
     7921 ggcgtgggta agaaaatcct ccagggcttt gaccgtggac aaatcccgga attcggcagg
     7981 agatgcgcaa gagacgacag cagccacttc tttgtgcagg gcagccacat agatggatac
     8041 cgcggctccc ccgctgaagc ccatgaaaaa gacccgcttc cggtccactt cggggcgaag
     8101 gtagagggtg ttcaatcctt tctccaggtc ccgagcccag ccgagaatgt cgaagtctcc
     8161 ttcgctctgc ccagccccgc ggaagttaaa gatcaaaacg aaaaatcctt tgtcgcaaaa
     8221 cctttcggcc agcaagggat accctcggtc atccggaccc ttgaccttcg ccggaatccc
     8281 atggcagagc gtcaaggcgg ggaaaggccc tgttgggcct gagggaaggt ggatttcccc
     8341 gcggatcgtc agtccgtcgc ttttccaaga aatcagttcg attttcatga ttccatgaat
     8401 tttgccaaag agttcacgga gggccacgca ttgaagactt attatctcca agaactccaa
     8461 cccggaactt gcagcttcaa actttttttc tacattttcc tcgtgctccg tctgagatag
     8521 gcttttagct catttttaaa tattttaaaa cctggctcat gacctctccg gccgagccat
     8581 ggatgaccac atcggcatac gaatcgtggt acgtcgggtc tcggttgata atgatcagct
     8641 tggctccggc ttccttagct tccaggggaa cggaggccgc gggctggacg accaaagagg
     8701 agccgatgac gatgaaaaga tcgcagttcc gcgcccggtg aaacgcctcc tcggtctctt
     8761 tggcaggcat ggattggcca aaggaaacgg tatcgggttt caggattccg ccgcaggcat
     8821 cgcagcgggg gaccttcaca ccccggagga gccatgactg gacctcctcc cgcgggtatt
     8881 tcttccggca ggaaaggcag gagacggaaa cggcggtccc gtgaagctcg atgacctttt
     8941 gcggcgagct tcccgcccgt tggtggaggt tgtcgatatt ctgggtgatc acgcagtcca
     9001 gcttccccat tttttccagt tcggctaccg cctgatgggc cgcattcggc tgggcgttct
     9061 tcaggggttc atagaattcc cggctcatct gccagtactt ctcccgggac tcctcgctgg
     9121 aaatgaactt ctggtagtag aaatcctcgg ggttgtattt ctgccagacc ccgccggggc
     9181 tgcggaagtc gggtattccc gactcggtgc tcaccccggc gccggtgaaa acgatcacct
     9241 ttcgggagct gcggatgaga tcggtgactt tacgaatgag ctcttccatg gttcactcca
     9301 ctagaaccca aagcgcagag tgcctagcgc atggcgtaaa aaaagctctg cccttcaaga
     9361 agattactct gtgctctgtg tgcatctgtg gtgattttgt ttggttacgg tcttcccaaa
     9421 acaaaaacac aggcaatcga gcccggaccc cccagagtgt gagtcaggcc gaaatcggcc
     9481 ttcttcacct ggcgcttgcc agcccgtccc cgcatctgat tgaccacatc ggccaccatc
     9541 cgtacccctg tggcacccac agggtggccg caggacttca ggcctccgct ggtattgacc
     9601 gggagctccc cgttcagtgc agtgatcccc tcttcgatga gctttcctcc ctctccccgc
     9661 ccgcagaaac ccaagtcttc atagttcacc agttcggtga tggtgaagca gtcgtggacc
     9721 tcagctacgt tgatttcctt gcgcgggtct ttgatgcctg cctgtcgata ggccatggcc
     9781 gatgcctcgc gggtggactt gaagcccagg aaatcgttat cctcctggaa ggccatgctg
     9841 aagtacccgt aagtgatggc caggcccata cctttgatga gaataaagtc ttttttcata
     9901 tccttggcat tctcaggagt ggtaaggatg accgcagcgg ccccgtccgt ggtcgggcag
     9961 cagtcaaaaa gaccgagcgg gtccaccacc ataggggctt tcaggaccgt ttcttcgttg
    10021 atgggcgagc ggaagtgagc ctttgggttc agggagccgt tgtaatggtt ctttactgcg
    10081 actttggcca gagtctgctt gccgtatccg tattggtgga agtagcgcgt ggcaatgggg
    10141 gcgaagattc cgggtgcggt ccttccctta gcaatcaccg ggtgcgtttc atgaatgtgc
    10201 cgggccacca gacttccccg aggtggcacc tctctcatct tttccacccc tacggcgagg
    10261 accatcttgt actctcccga ggcaacgccg aaggcggcat tccggatggc ttccatcccc
    10321 gaacagcaat aggctgaaac gcgggtgacc gggcggggat agaagtttaa aggctcggaa
    10381 agggaagcgc ctgcgtcgcc ttccagtccg gtcaggccgg gggaaagggt tcccagccag
    10441 gccgcctcca tatccttcgt acccagaccg gcatcctggt aggcctcata gaccgcctcc
    10501 agggccatgt cctggtaggt catctcgaaa ttctccccga acttcgtcgc cccaacgccg
    10561 acgatggcga ctctatctct aatgctcatc tctccctcct tttttgagac ccaaaacctg
    10621 taactctaaa tttttcctct gtgcttcagg ggtgaagaat ctattccaat gggcgaagtt
    10681 tccagaaata attgtagaac ctatctccct gatggaaccg gcgcaaggtg agttccacgg
    10741 gcatgccgat cttgatctgg cccgggtcgc agtcggtcac ctggccgtag aaacggcctc
    10801 ccccttccat atccgccacc gccatcaccg tcgggggatc aggattgggg aaaagatggt
    10861 ccttagcgaa ggtgtagatt tttccttttc tgggcagctt ggcgtcttcg tattcgtctt
    10921 tggcatagca attcaagcag accctgcgcc gggggaaggc gaaggttccg cacttcttgc
    10981 aacgggtacc gtaaagctga aggttttgtt tcttctcctt ccagagaagg gcccaggaag
    11041 aataaggctc caggggctca ttggggaggg tattctggaa tttgagaaat ttttcgtaat
    11101 tggccaggga tttcttggaa gcctgatagt aggaaacccc ccgccgggcc ttcagacgct
    11161 cgatctccgg agtgacctga aggaggaaag catctgcgtt tccggccccg taactgcaga
    11221 gaaggacccg ttcccccggt ttggccttct ccagggcggc ggccaggagc atcaaggggg
    11281 cagccgcccc cgtatctccc acggtggtta gaaggggatc ctccgcgaat gaaccggcgg
    11341 gtaaattcag ggacttggtt agccgggcat agacgcggct gtccggcgca gggatgacga
    11401 attgggagac gtcggaaatt ttcatttcat acttcttgag gactccctgg atgcaagctg
    11461 ggaccattcg ggcatatccg taggcctgga caaaagcggc gtctcctttc tgaacgaaat
    11521 catcctcctg ctttctccag gtatccatga attcctcgga taaagaatag accccctcga
    11581 tggaggccgc cactccggag cttccgatca gaaccgccgc ggccccgtct ccgaagagct
    11641 gttctgagtc cgatcccggt tcgccccggc gagtgtccgc cgcagtgact agcaggttcc
    11701 tggcgctgcc accttttact gcgtccaacg ccgctttcat tcccgcggtt cccgcgcgga
    11761 tcgaggagag gtggtccgcc gtataaatct ccacgggaaa atccagggct gtagcaacca
    11821 gggtggaagc ctgtttttcc agataggggg atgtggtaga ggcaaagatc aatccatcca
    11881 ccgtttttgg atcggttccc gccaggctgt tcagtgctgc ttcagcagcc atggtcaggc
    11941 tgtcttcgtc atggttggct accgcccgct ctccggagat aaaccgagga ccccaggccc
    12001 tggccatcac atcccgcccc aggcggtact tggggatgta caccccaatg ccggaaatcc
    12061 ctgccattga cccctccttt tctctcaggt tgaaccatcg actgattccc caattcaaat
    12121 gtacctgatc atgcttttca tgcttcggga aagggaaagt ttccctcgtt aaggaaattc
    12181 cgcgaacaaa ttagcataga agaaggtcta atgcaatcct gacatgtctg ccatcatcaa
    12241 cccgattggc ttttaggcca ggcctatgaa taaaaggttg ccccgtggtt ccagggtcac
    12301 gctggaattc caggaaatga aggcggaatt ggagaggatt
//
LOCUS       DASPRR010000152        16446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1328217, whole genome shotgun sequence.
ACCESSION   DASPRR010000152 DASPRR010000000
VERSION     DASPRR010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16446)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16446
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328217"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VEL68_05750"
     CDS             <1..702
                     /locus_tag="VEL68_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HXZ35503.1"
                     /translation="AIATGPGEFLGKEAVEGRAADLAKDLARAIKEKRRYPATDRDLR
                     FYQFMGALVKSEKDTLMKHDFKHWQEHGFYDSFEKYIQQSTTEVSFDQGVRDAWIKEM
                     IQEQKAKKSGKSAIPKKEPGAARPLPKTCRELIQSMPQGFQPEAAGDLTAKLQFEVQG
                     EENFVSHLKIAGGACTYQEGPADKPNLIIKTPADVWLKISRGELSGQKAFMEGQYKVE
                     GDMTLLMKLNRLFSA"
     gene            856..996
                     /locus_tag="VEL68_05755"
     CDS             856..996
                     /locus_tag="VEL68_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HXZ35504.1"
                     /translation="MENQEAYRKSKGRVEAKFGVYWHLTVYLVVIAVLVIINLSSRPE
                     YL"
     gene            1250..1393
                     /locus_tag="VEL68_05760"
     CDS             1250..1393
                     /locus_tag="VEL68_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35505.1"
                     /translation="MKNLLLAILMIMAFPPLLRGQSPLPKSEPDVALVFSSTVQGEVE
                     PCG"
     gene            1475..2617
                     /locus_tag="VEL68_05765"
     CDS             1475..2617
                     /locus_tag="VEL68_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXZ35506.1"
                     /translation="MDTGDLLYSRDSVETPNARQIGNLKAELYMKAYNLMGYDAFTPG
                     ELDLSFGVGDLIKMSQQANFPLLAANLIHTGSMQPVFKPYVIKEVGGIKIGIFGLVSG
                     QSSPWGPPEEKDKFKITDPFEAAKNAVGVLKRECRLIVALAHMEADEQKLLVERVPGI
                     HFVINGHLSHAHSTPLLVGQSQILIAGARGEYLGQVDLFQQKMKIYSRYQLNALKADC
                     AEKPEVQAWVAQYKDRLQSVQQPLTMAGGPPMGPSGSASILAVPQLLAFMGEKSCQTC
                     HPREHEHWATTAHARAYWTLVERNKASDPSCLPCHTTGYGSFQDPRARFENVQCEACH
                     GPGEAHPNVRKGMERAEEYECRKCHTATNSPSFNFDIYVRGIIHPR"
     gene            2674..3210
                     /locus_tag="VEL68_05770"
     CDS             2674..3210
                     /locus_tag="VEL68_05770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXZ35507.1"
                     /translation="MKKACQRILVTVLACAGFFLLPGLQHASGQTTFQAYAQPQAMPA
                     FLLEDLQGKRVDIRDHRGQVILLNFWATWCPNCRQEGPSIGKLYALYKTRGLILYRID
                     SKEDPQTVRQFLEKESLEFPVLLDRDGRVERLFGVWVHPTSYLIGRKGTVRYRVMGVG
                     DWTSLQGTSVIDQLLSER"
     gene            3214..3774
                     /locus_tag="VEL68_05775"
     CDS             3214..3774
                     /locus_tag="VEL68_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35508.1"
                     /translation="MKEKLFFPVLILAFLGVSGCGGVIPIMGEKAPIPLQKANPEAVW
                     VENEGLQIVWEDAKDARDFYRGGVQRKSQAEKRFQEKGYPEALKFYQVSNDFFTKLLT
                     YLNEDMAEFTLYEGTDILFFPNLLLADNHLKIGLIQRETGHEASAQSSWKKAQGWVQK
                     SLQSEKTEWGLSLEKEVMSLLGSKKN"
     gene            4086..5177
                     /locus_tag="VEL68_05780"
     CDS             4086..5177
                     /locus_tag="VEL68_05780"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXZ35509.1"
                     /translation="MKFRDRTKLRFRILLLLGVASGMTGWIYTGLIQPSSSGHHPWVG
                     IFTGVGVGVTIGGSELFFLRAWIKRKSFAVALLIRTAYYLTVIMGIGFTLWGFGEEGG
                     FIGLSLGQLWGYHDAILFSLLKSFAFSLFFQINGLIGGRVLLSFFTGKYHQPVEENRI
                     FMFLDLKSSTAIAEKIGHIQFHKFINDFLHTISEPIIANKGEIYKYVGDQVIITWKLK
                     EGLRENQCLRLFFEASDRVDRERDSFEKKYGIVPEFKAGMHCGRVVSGEMGDTKREIA
                     FLGDVINTTARIEKECSPKSRRLLISSDVFKKLHLGEEYRHEEMGNIQLRGKTTGIDL
                     YAIERNSREEGVAGPHLGIGTPSRGKAGP"
     gene            5268..5915
                     /locus_tag="VEL68_05785"
     CDS             5268..5915
                     /locus_tag="VEL68_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXZ35510.1"
                     /translation="METAFFLGLTSGGSCLASCGPLAAALLASEEIPLRRSSALLSVF
                     LSGRLLGYLCWAVLSWLLGWLISQSTKGFALFSAADLALGAWLIYYGIRPPVPSHGSC
                     PGSTLNSLNQSGSRHGAFFRGGLWGFLSGLQVCAPFLTAFAEAARTGSLGGSLLFFLL
                     FYLGTGIWFIPFPFIGTLGRFRQIAQVARFLTLPLGAYFIYSGIISIAGGMTFYG"
     gene            5908..7089
                     /locus_tag="VEL68_05790"
     CDS             5908..7089
                     /locus_tag="VEL68_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ35511.1"
                     /translation="MDEMLAKTKDRIPPVPGLEKSWGTSLLWTLPMVLWALMMFSRGF
                     FQGDLAQKLSVIITVGFLAGIFFWMIKSGETYRPRRTFYVALGFLFPVGFISELIAQR
                     GSMSISLEQMLLGNTPFCHLALPMMALPAVLSKTIIFPGSILPKAENPHAFGAVLALW
                     VGATLILGRGWCSWGCFFGGIEEGFASLRKKPLWPKIDRRWTLLPWAVLTLVVVGTAI
                     SLAPFYCQWLCPFKTVTEFPAVTSVQTAIQFGIFTTLFAGIVVVLPLLTKKRTQCSFF
                     CPLGPVQALANKINIFELKTDPERCVSCGLCEKACPFMAVERGEGKERQVNMYCARCA
                     ACADVCPKGAVRFRIKGTPFTVNAAAPRMMFLLATWSFATMFGGSIIANSLSKIVGHL
                     L"
     gene            7103..7612
                     /locus_tag="VEL68_05795"
     CDS             7103..7612
                     /locus_tag="VEL68_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35512.1"
                     /translation="MKRLLLFLGYGAAALTILFALALPIKGFPVFLSALGNLGLKIAP
                     WYSGGEVASTLDRGSYQIKVYHPVYPALVGEGEKGFVQLVWQPRSALPPRVQEEIDLN
                     GDGKPDCEVSFSNPPEEAGAPLLTVNPKSPWVSPVHNSPTTSFEGILVERVKDAMYVR
                     IPLKKMGSS"
     gene            7703..8473
                     /gene="pstB"
                     /locus_tag="VEL68_05800"
     CDS             7703..8473
                     /gene="pstB"
                     /locus_tag="VEL68_05800"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXZ35513.1"
                     /translation="MGNPDHSIKMKAEGLNFFYGAFQALKKVNLEIKERQITSLIGPS
                     GCGKSTFLRTLNRMNDLIPYSRVIGTVLLDGKNIYDPEVNPVNLRQRVGMVFQRPNAF
                     PKSIFENVAFGPKVLGNRHKSDLQEIVERSLQGAALWNEVSDHLQHPATELSLGQQQR
                     LCIARVLAIQPEVILMDEPCSALDPQATLRIEELMQELKEKYTIVIVTHNMQQAARAS
                     DWVGFFLLGELLEYGPSKEIFTNPRDPRTESYITGRYG"
     gene            8475..9311
                     /locus_tag="VEL68_05805"
     CDS             8475..9311
                     /locus_tag="VEL68_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ35514.1"
                     /translation="MTPTRATLRILSLALCLSQILLPGCHRKQGGVSVVGSTSVQPFA
                     EALAEEFMVGHSAEKIFIQGGGSTAGIQAVFTGAAQVGMSSRKLEENEKALIAVPIIY
                     DAIAVIVHRTNFLDSLSMDQVREIYAGRITRWKEVGGTDRAITVVTREEGSGTRETFQ
                     NLVMGKKEEISLGALVQDSNGAIRQVVADDPNAIGFISLGLVNERVKALKLDGTEPTV
                     ENVRAHRYQIVRPFLFVFKAPPQGIAKEFLEYVLSPKGQKLLMQEGLVSMDQNLPSEA
                     RK"
     gene            9316..10161
                     /gene="pstC"
                     /locus_tag="VEL68_05810"
     CDS             9316..10161
                     /gene="pstC"
                     /locus_tag="VEL68_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXZ35515.1"
                     /translation="MNKEKFLERGFLLVALSSISVLALIAFFIFEEGLPLMWKVGLQS
                     FLLGDRWFPSKGSFGIFPMIVGSFWVTLGALVVGVPLGLACAIFLCELTSPKVGMVLR
                     PMIQLLAAIPSVIYGFWGLIVLVPLIRNHLGGPGLSILAGSFVLGIMILPTIISISED
                     SLRALPRIYKEGALSLGATHWQTIWRVLLPAARSGIVASIILGMGRALGETMAMIMIL
                     GNAAQMPHSILNSARTLTTNIGIEMGYASGDHRAALFATGVVLFFIIMGLNSLALVIS
                     RKGRA"
     gene            10163..10996
                     /gene="pstA"
                     /locus_tag="VEL68_05815"
     CDS             10163..10996
                     /gene="pstA"
                     /locus_tag="VEL68_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXZ35516.1"
                     /translation="MKISRHFTQEIARVVIGVAAFSTLAVMFWILYQVLREGLPLLHL
                     SFFVEPPREMGRAGGISSTIIGTLALTVVAIVIATPLGVGTAIFLREYTRESRISRVI
                     RFGTDCLAGVPSIIFGLFGFVFFVIFLGMGWSILSGGLTLAAMILPTIICTAEEAIKS
                     IPYSFREVSYGLGGTRWQTIRRVVLPNAFPGILTGVLLGVGRSVGETAAVILTAGSSF
                     LMPDSIFSPIRTMAVHFYILAREGISMEMAYATGAGLIILILIINTVANWLLFRYVHR
                     G"
     gene            11010..11771
                     /locus_tag="VEL68_05820"
     CDS             11010..11771
                     /locus_tag="VEL68_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HXZ35517.1"
                     /translation="MENQFKIVIRDFEVYYGSAMALKGLNLSIRPSQIFAVLGPARSG
                     KTTLLKSLNRLTDLIFGANHRGQIFIDGQEIYGENVSLPELRRRVGMVFDLPTALPMS
                     IFENIAYGPRLKGIAGRTRLGEIVEASLTAAVLWDEVKDRLHSSALRLSGGQQQRLCI
                     ARMLALEPEILMLDEPCSGLDPISTASIERSLMSLKEKYTILLVPHNIQQASRVADRV
                     GFFLGGELIEEGPSEEIFTRPRMKKTEDYISGRFG"
     gene            11896..12594
                     /gene="phoU"
                     /locus_tag="VEL68_05825"
     CDS             11896..12594
                     /gene="phoU"
                     /locus_tag="VEL68_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXZ35518.1"
                     /translation="METRFQQELNHLKEDLLKMAGLAERAISNAIEALVKRDTGLAER
                     TIAEDEKINKMELIIDEWCLKLLALHQPMAADLRFITSAMRINTELERIGDLSVNIAE
                     RVISLNQEPQLKPYIDIPRMAEITQGMVKDVLDAFVKGDADLARSVCERDDQVDALND
                     QVFRELLTYMMSDPKTITRAVHLIIVSRCLERIADHTTNIAEGVIFMVKALVIKHHAD
                     VQDKPEKKSKEGKK"
     gene            12799..13119
                     /locus_tag="VEL68_05830"
     CDS             12799..13119
                     /locus_tag="VEL68_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35519.1"
                     /translation="MRNGIGVVLILAFFFCPWMTQAQTQESTPAQKKIMMERGEKVTG
                     KRLELVKKVIESYRDCEVLAKGNELTGLRINGKLYSDFTLVVRDAKRTIIVATPDGVV
                     SFDY"
     gene            complement(13186..13629)
                     /locus_tag="VEL68_05835"
     CDS             complement(13186..13629)
                     /locus_tag="VEL68_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35520.1"
                     /translation="MLSQALVELIEKNAEKIAKEWLNDVRKNALTPFYHTFDEDHLYQ
                     RATSMYKGLSQFLSVQTPKEQISKYYKKYGQDRCQEGFPLSELVYSFILFRRHLWLFI
                     LHSGFFDTAYELLRALELNNRVILFFDRGLVNLVKGYEECQRSKK"
     gene            complement(13640..14713)
                     /gene="mtnA"
                     /locus_tag="VEL68_05840"
     CDS             complement(13640..14713)
                     /gene="mtnA"
                     /locus_tag="VEL68_05840"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXZ35521.1"
                     /translation="MGIKTLEWKKGKLYLLDQTKLPLSEAYVACSTYQDAARAIRKMV
                     VRGAPAIGVAAAFGMVLGIRQFKSGTRAALKKHVDRVAQALVSTRPTAVNLVWAVERM
                     RRLVFKKMGGDSQILREELEKEALRILEEDLEANREMGRQGQVLIPDGVSVLTYCNAG
                     ALATAGYGTALGVIRAAREAGKRIHVYVGETRPLLQGARLTAWELMRDRIPFTLIADN
                     MAGWLMAQGKIHLAITGADRIAANGDAANKIGTYSVALLCRAHRLPFYVAAPISTIDL
                     STARGEKIPIEERASTEVTTLRGKRMAPHAVKVYNPAFDVTPHRFISAIITERGIVQS
                     PYRLNLKRILSGATPDDDQRLIG"
     gene            15018..16376
                     /gene="purB"
                     /locus_tag="VEL68_05845"
     CDS             15018..16376
                     /gene="purB"
                     /locus_tag="VEL68_05845"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXZ35522.1"
                     /translation="MAGHPIDYYVYGGSWGTDEGRKIFSEEQMIDRWLKIEAALAEAE
                     SEMGIIPEWAAKEISAKASVERIDLNLARAEQQKTRHSLVPVIRAFARACGEKAGEFV
                     HYGPTTQDIIDTGNALALREVMDAVKRDLEAIEKRLVQITLEYADTPMAGRTHGQHAL
                     PITFGFKTASWLQEVRRHLDRLEEGRIRILVGELAGAVGSYASWGEQGRELERRTVAR
                     LGLGQSDIPWQSSRDRSAEAVLLLAMICSTLGKIAGEVYLLQKTEVDELAQGFEMGRV
                     GSSTMPHKRNPETAEGIKGISRLVRYRAAGILEAMEGEHERDASNWKTEWVIIPEVSI
                     YTLAALSKTRELLGALAVKRENMRANLDRTRGLILSERAMFVLGEHLGKQTAHEVVYE
                     ASMKAFETGKDLTSALAADSRVGKVLSEEAIRKFMDPVTYTGLCAQLAREVASRERKK
                     KG"
BASE COUNT         3643 a         4349 c         4782 g         3672 t
ORIGIN      
        1 gccatcgcta ctggaccggg agaattcctg gggaaggaag ccgtcgaggg cagggcggct
       61 gacctggcca aggacctggc gcgggcgata aaggaaaagc gtcgctatcc ggccacggac
      121 cgggacctcc gtttttacca attcatgggg gctttggtga aaagcgagaa agataccttg
      181 atgaagcatg atttcaagca ttggcaggag catgggttct atgacagctt tgaaaaatat
      241 atccagcagt caaccaccga ggtctccttc gaccaggggg tgcgcgacgc ctggatcaaa
      301 gaaatgattc aagaacagaa ggcgaagaaa agcgggaaat ccgcaattcc gaaaaaggaa
      361 cccggcgcgg cgcgcccatt gcccaaaacc tgccgggagt taattcagtc catgccccag
      421 ggcttccagc cggaagcggc cggagacctg acagcaaaac tccagtttga ggtccagggc
      481 gaagagaatt tcgtctctca tctgaagatt gccggagggg cctgtactta ccaggagggc
      541 ccggccgata aaccgaatct gatcatcaaa actcccgcgg atgtatggtt gaagatttcc
      601 cggggcgagt tgagcggcca gaaggcgttc atggaagggc agtacaaagt tgagggagat
      661 atgactctcc tgatgaaatt gaaccggctc ttttcggctt aggcaccaag agactgtgtc
      721 gtaattcgtc atgccggcgg aagccggcat ccaggattca agtaattaga ataaaatcaa
      781 acactggatt ccggctttcg ccggaatgac gccctttccc ttaattgcga cacagcccca
      841 aggggaggga gaaaaatgga aaatcaggaa gcttaccgaa agtccaaggg gcgggtggag
      901 gccaagttcg gggtctactg gcatttgacc gtgtacctgg tagttatcgc ggtcctggtt
      961 atcatcaatc tcagtagccg gcctgagtac ctgtagttca aatggcccct cttctgatgg
     1021 ggaatcgggg tctttttcca tggcctggcg gtattccgca ttttttcggg cagattggca
     1081 gccaggaagg aaagaatgat tcgggaggag atgaaaaagc attttcaacg gtaagaacac
     1141 tgcgataggg attattttag ccatgcacgg taaaccttcc gcgaactgga aacccagggc
     1201 cgcccagtaa tcaaggtatc cccagggctg aaaaaggaag gagtcagtga tgaagaacct
     1261 tctcctcgcg attctcatga tcatggcctt tccgcctctc ctgcgggggc agtctcctct
     1321 cccgaaatcc gaaccggacg tagccctggt cttttcttcc accgttcagg gcgaggtgga
     1381 gccctgcggg tgacaagcga gtcagcttgg cggtctggcc aagcgagcga gcttcgtcga
     1441 gaatctgcgg ggcgaaagga gggaggttat tctcgtggac acgggggatc ttctctattc
     1501 ccgtgactcc gtggagaccc ccaacgcgag gcagatcggg aacctcaaag ccgaactcta
     1561 catgaaagct tacaatctca tgggctacga tgcttttacc ccgggggagc tggacctttc
     1621 cttcggcgtg ggagacctca tcaaaatgag ccaacaggcc aacttccccc tcctagcggc
     1681 caatctcatc cacacagggt cgatgcagcc cgttttcaaa ccttatgtaa tcaaggaggt
     1741 cggggggatc aagatcggca ttttcgggct tgtctccggc cagtcttctc catggggtcc
     1801 ccccgaggag aaggacaaat tcaagatcac cgaccctttc gaggcggcga aaaatgcggt
     1861 gggcgtgttg aagagggagt gccggctgat cgtggccctg gcgcacatgg aggccgatga
     1921 gcagaaactt ctcgtggaaa gagtgccagg aatccatttc gtcattaacg gccacttgag
     1981 ccatgcgcat tccaccccgc tgctggtggg acagtcccag attctcatcg ccggggcccg
     2041 cggtgagtac ctcggtcagg tggatctttt tcagcagaag atgaagatct attcccgtta
     2101 ccagctgaac gctctgaagg cggattgcgc ggaaaaaccg gaagtccagg cctgggttgc
     2161 ccagtacaag gaccgattgc agagtgtcca gcagcccctg acgatggcag gcgggcctcc
     2221 tatgggtccg tccggttctg cttccatctt ggccgttccc cagctcctcg ccttcatggg
     2281 ggaaaagagc tgccagacct gccatccccg cgagcacgag cactgggcga cgaccgcgca
     2341 tgcccgggcc tactggacgt tggtcgagag aaacaaagct tccgatccct cctgcctacc
     2401 ctgccatacc acggggtacg gttcttttca agatccccgc gcccgattcg aaaatgtcca
     2461 atgtgaagcc tgccatggcc ccggggaagc ccatcccaac gtgcggaaag gcatggagag
     2521 agcggaagaa tacgaatgcc gcaagtgtca caccgcaacg aacagcccga gcttcaattt
     2581 tgatatttat gtgcggggaa ttatccatcc tcgatgaacg aacgctccgg aacatcttta
     2641 cgatgacaaa cggaaaggag gaagtctaaa aaaatgaaga aggcatgcca aagaatatta
     2701 gtgacggtat tagcctgcgc cggcttcttc ttgctgccgg gacttcaaca tgcttccgga
     2761 caaacgacct ttcaggccta tgcccagccc caggcaatgc ccgccttttt gctggaggat
     2821 ctgcagggaa agcgagtgga tatccgggac catcgaggcc aggtcattct tttgaatttc
     2881 tgggctacct ggtgtccgaa ctgccgccaa gagggtcctt ccataggcaa gttatacgct
     2941 ctgtataaga cccggggttt gattctatac cgcatcgaca gtaaagaaga cccccagacc
     3001 gtccggcagt ttctggagaa agaatccctg gagttcccgg ttcttcttga ccgggacggg
     3061 agggtggaac ggctttttgg agtgtgggtg catccgacca gctatttgat cggccgcaag
     3121 ggaacggtgc gctacagggt catgggggtc ggggattgga ccagcctgca aggcacgagc
     3181 gtcattgatc aactcctaag cgagaggtga gagatgaaag aaaaactctt ttttccggtt
     3241 ttgatcctgg cctttctggg ggtttccggt tgcggagggg tgattccaat catgggagaa
     3301 aaagccccca tccccctgca gaaagccaat ccggaagccg tttgggtgga aaatgaaggg
     3361 ttgcagatcg tatgggaaga tgccaaggac gcccgggatt tctaccgcgg aggggtgcaa
     3421 cgaaaatccc aggcggaaaa acggttccag gaaaaaggct atcccgaggc cctgaaattt
     3481 taccaggttt ccaatgattt ttttaccaaa ctcttaacgt acctgaatga ggacatggct
     3541 gaatttaccc tctatgaagg aaccgatatc ctctttttcc ccaatctcct cctggccgac
     3601 aatcacctca agatcggtct gatccagcgg gagacggggc atgaggcctc cgcgcagagt
     3661 agttggaaaa aggctcaagg gtgggttcaa aaatccctgc agtcggagaa gacggagtgg
     3721 ggcctgtcac tggagaagga agtcatgagt ttgttgggaa gtaagaagaa ttgattcttc
     3781 ggcctgcgat gggccgatcc tgcgacattg ggcatcgatt cagtagcgca acgaggtgta
     3841 aaatggagag gaagaaaagt gcagggtagg aaggaattag tgatcgtcag gcggtgaaag
     3901 cgacctttga ttcttacgag gtgagggaga aaaaatagaa acggagaaga gaaaggcggg
     3961 agggggccat tgcagaattc caaattttcc cgattcaccc tcctatgttc ttcgataccg
     4021 actaggcgcg ggaggaagca ggattatccc tcaaggtttc ctcccgttga agcgagaatc
     4081 ggagaatgaa attcagggat cgaaccaaat taaggtttag aatccttctc ctcctgggag
     4141 tcgccagcgg gatgaccggg tggatatata ccggcctgat tcagccttct tcatccggtc
     4201 atcatccctg ggtcggcatt ttcacgggag tcggggttgg cgtgaccatc ggcggcagcg
     4261 agttattttt cctccgcgcc tggattaaga ggaaaagttt tgcggttgct ctgttaatca
     4321 ggacggccta ttatttaacg gtcattatgg gtatcggctt taccttgtgg ggattcgggg
     4381 aggaaggagg attcattggc ttatctttgg gacaattatg ggggtatcat gatgcgatcc
     4441 ttttctccct tcttaaatcc tttgccttct cgcttttttt ccaaatcaat ggactcatcg
     4501 gggggagagt tctcttatcc ttttttaccg gcaagtatca tcagcccgtg gaggaaaata
     4561 gaatcttcat gtttctcgac ttgaaatcgt cgacggctat tgccgaaaag atcgggcata
     4621 tccagttcca taaattcatc aacgattttc ttcataccat ctccgagccc attattgcca
     4681 ataagggcga aatctataaa tacgtgggcg atcaagtcat cattacctgg aagttgaaag
     4741 aagggcttag ggagaatcaa tgcctacgct tattctttga agcttcggac cgcgttgacc
     4801 gggaaagaga ttcatttgag aagaaatatg gaattgtgcc agaattcaag gcgggaatgc
     4861 attgtgggag agtggtctcc ggggaaatgg gagacaccaa gagagaaatt gctttcctgg
     4921 gagacgttat taacactacc gcgcggatcg aaaaggaatg cagtcccaaa tcgcggaggc
     4981 tcttgatctc gagcgatgtt tttaagaaat tgcatctggg ggaagagtat cgccatgagg
     5041 agatgggaaa tattcagctg cgggggaaga cgacaggaat cgacttgtat gccatcgaaa
     5101 gaaattctcg agaagaggga gttgccgggc ctcatctagg gatcggaact ccttcgcgcg
     5161 gcaaagcggg gccttaagct gcggggggat cgggtaatca atcgttgcct cgccaaacga
     5221 tccggcccaa tgtttcggat aaactttacg ggggggaccg ataattcatg gagacggcct
     5281 tttttctggg tttaacttcg ggcggatcct gcctggccag ctgcggccct ctcgccgcgg
     5341 cgttgctggc gtccgaagag attcccttgc gacgcagcag cgctctcctt tccgtcttcc
     5401 tttcggggag actcctcggg tatctctgct gggcggttct gtcctggctt ctgggatggc
     5461 tgatctctca aagcacgaaa gggtttgccc tcttctccgc cgcggatttg gcgctggggg
     5521 cctggctgat ttattacgga atccgcccac ccgttccctc tcatggcagc tgtccgggct
     5581 cgactttgaa ttccttgaat caatctggga gcaggcatgg ggcgtttttt cggggggggc
     5641 tctgggggtt tctgagcggc ctgcaggtct gtgctccatt tttaaccgcc tttgccgaag
     5701 cagccagaac cggaagcttg ggcggctcgc tccttttttt ccttcttttt tacctgggga
     5761 cgggaatttg gttcattccc ttcccgttta tcggaactct cggacgtttc cgccagatcg
     5821 cccaggtggc ccgtttcctg actctgccgc tgggcgctta ttttatctat tcgggaatca
     5881 tttcgatagc aggaggaatg acattctatg gatgagatgc tggcaaagac aaaggatcgg
     5941 attccgccgg tgccgggact ggaaaagagc tggggaacct ctctgctctg gacccttccc
     6001 atggtcctct gggccctgat gatgttctcc aggggatttt ttcaaggtga tttggcccag
     6061 aagctttccg tcatcataac cgtaggcttt ctagccggaa tattcttctg gatgataaaa
     6121 tccggcgaaa cctaccggcc gcgccggact ttttacgtcg ccctggggtt tctcttcccg
     6181 gtgggtttca tctccgagct catcgcccag cgggggtcga tgagcatttc tctggagcag
     6241 atgctcctcg gcaatacccc cttctgccac ctggcccttc ccatgatggc gctccccgcc
     6301 gttctctcca aaaccatcat ctttcccgga agcatcctgc ccaaagccga aaacccgcat
     6361 gccttcgggg ccgtgctggc actgtgggtg ggggcgaccc tgattttggg ccggggctgg
     6421 tgcagctggg gatgcttctt cggcggaatc gaggaagggt tcgcctcttt gcggaaaaag
     6481 cccctttggc cgaagatcga ccggcgatgg accctccttc cctgggcggt gctgactttg
     6541 gtagtcgtag ggacggcaat ttcccttgcg cctttttatt gccaatggct ctgccccttc
     6601 aagaccgtaa ccgaattccc cgccgtgacc tccgtgcaga ccgccataca gtttggaatc
     6661 ttcacgaccc tctttgcggg gatcgtagtc gtccttcccc tcttgaccaa gaagcggacc
     6721 cagtgctctt tcttttgccc cctgggcccg gttcaggcct tggccaacaa gatcaacatc
     6781 ttcgagctga agaccgaccc tgagcgctgc gtttcctgcg ggctttgcga gaaagcctgc
     6841 ccgttcatgg ctgttgagcg gggagaagga aaggaaaggc aagtaaacat gtactgcgcc
     6901 cggtgcgctg cctgtgccga tgtttgcccg aagggggcgg tccgcttccg catcaaaggg
     6961 actccgttta cggtcaacgc cgctgccccg cgcatgatgt tcctgctggc cacctggagt
     7021 ttcgccacca tgttcggcgg aagcatcatc gccaatagtc tgtccaaaat cgtcgggcat
     7081 cttttataag agagggaatc acatgaaaag actgcttctg ttcctgggat atggggccgc
     7141 agccctgacg atcctcttcg ccctggctct cccgatcaag ggtttcccgg ttttcctgtc
     7201 ggcgctcggg aacttgggcc tcaagatcgc cccctggtac tcgggcggcg aggtggcctc
     7261 gacactcgat cggggcagct accagattaa ggtttatcat cctgtctatc cggcgctggt
     7321 gggggaaggg gaaaagggat ttgtgcaatt ggtgtggcag ccaaggagcg ctttgcctcc
     7381 tcgggtccag gaggagatcg acctgaacgg ggatggcaag ccggactgcg aggtttcttt
     7441 ttccaacccc ccggaagaag ccggcgcccc attgcttacc gtaaatccca agtctccctg
     7501 ggtttctccc gtgcataatt ctcccactac ctctttcgaa gggatcctcg tcgagagagt
     7561 gaaggacgcc atgtatgtcc gcatccccct aaaaaaaatg ggctcatcgt aggcgaagta
     7621 tccagcccag ccccgaaagg gtcacagaaa ggcatgattg ctcttgatca attgcccgtt
     7681 ttgagtgata ttattaattc acatgggcaa tccagatcac tcgataaaga tgaaagcaga
     7741 aggcctgaat tttttttacg gggcctttca ggcattgaag aaggtaaacc tcgagataaa
     7801 agaaagacaa atcacctccc ttatcggccc gtccggttgc ggaaagtcca ccttcctgcg
     7861 tactcttaac cgaatgaatg acctcatccc ctacagccgg gttatcggta cggtgctgct
     7921 ggatgggaag aacatctatg accccgaggt caatcccgtc aacctgcgcc agagggtggg
     7981 aatggtcttc cagcgaccca atgcctttcc caaatccatc ttcgaaaacg tggccttcgg
     8041 ccccaaggtt ttgggcaacc ggcacaaatc cgacctgcag gagattgtgg agcgaagcct
     8101 tcagggggcc gctctctgga atgaggtgtc cgatcacctc cagcatccgg ccacggaact
     8161 ttctctcggg cagcagcaac gactctgtat tgcccgggtt ctggccattc agcctgaagt
     8221 gatcctcatg gatgagcctt gctcggctct ggacccccag gccactctgc gaatcgaaga
     8281 gctgatgcag gagctgaagg agaaatacac catcgtcatc gtgacccaca acatgcagca
     8341 agcggcccgc gcctcggact gggtaggttt ttttctcctc ggggagctgc tggagtacgg
     8401 ccccagcaag gagattttca ccaaccccag ggacccacgg acggagagtt acattacggg
     8461 aagatacgga taaaatgacg cccaccagag cgactctacg gattctttct ctggctctct
     8521 gcctctccca gattctccta cccggctgcc accggaaaca gggcggggtg tccgtggtgg
     8581 gctcgacttc tgttcagccc tttgccgagg ccttggccga agaattcatg gtcggccatt
     8641 cggcggaaaa aatcttcatc cagggcggcg gctcaactgc cgggatccag gcggtcttta
     8701 cgggtgctgc ccaggtgggg atgtcttccc ggaaactgga ggagaacgaa aaggcgctga
     8761 tcgccgttcc catcatttac gacgccattg ccgtaatcgt ccatcggact aatttcctgg
     8821 acagcttatc catggaccaa gtccgggaga tttatgccgg gagaatcacc cgctggaaag
     8881 aggtaggcgg cacggatcgg gccatcaccg tggtgacccg ggaggagggg tcgggcaccc
     8941 gggaaacctt ccagaacctg gtcatgggaa agaaggaaga gatcagcctg ggggctctgg
     9001 tccaggattc caacggggcc atccggcagg tcgtggccga tgaccctaat gccattgggt
     9061 tcatctccct ggggctggtg aacgaacggg tgaaggccct gaagctcgac ggaactgagc
     9121 ctaccgtcga aaatgtccgg gctcatcgct accagatcgt ccgtcctttt ctctttgtgt
     9181 ttaaggcccc cccgcagggg atcgccaaag agttcctgga gtacgtgctg agcccgaaag
     9241 gacaaaaatt gcttatgcag gaagggctgg tcagcatgga tcaaaacctc ccgagcgagg
     9301 cacggaaata gacggatgaa taaagaaaaa ttcctggaaa gagggttcct cctggtggcc
     9361 ctctcctcca tttcggtcct ggccctgatc gcctttttca tattcgaaga gggccttcct
     9421 ctcatgtgga aggtcggcct ccagtccttc cttctggggg atcgttggtt cccgagcaag
     9481 ggttctttcg ggattttccc catgatcgtg ggttctttct gggtgacgtt gggagcgctt
     9541 gtcgtgggcg ttcccctggg actggcctgc gccattttcc tctgcgagtt gacgtctccc
     9601 aaggtgggca tggtcttgcg tcctatgatc cagctcctgg ccgccatccc ctcggtcatc
     9661 tatggattct gggggttgat cgtgctcgtg ccgctcatcc gaaaccacct ggggggtccg
     9721 ggtctgagca ttctggccgg gtccttcgtt ctggggatca tgatcctgcc caccatcatc
     9781 agcatctcgg aagattctct tcgggccttg ccgcggattt acaaggaagg ggcgctttct
     9841 cttggcgcga ctcactggca gacgatctgg cgggtcctgc tgcccgctgc ccgctcggga
     9901 atcgtggcca gcattatcct ggggatgggg agggcgctcg gggagaccat ggccatgatc
     9961 atgatcctgg gaaacgcggc gcagatgccc cattccatcc tgaacagcgc ccggaccctg
    10021 acgacgaata tcggcattga aatgggctat gcctcggggg atcaccgagc ggctctcttt
    10081 gccacgggtg tcgtcctttt cttcatcatc atgggcctga actctctggc cctggtcatt
    10141 tcccggaagg ggagggcgtg aggtgaaaat ctcccgtcat tttacccagg aaatagccag
    10201 ggtggtgatc ggggtcgcgg ccttctccac cctggcggtc atgttctgga tcctttacca
    10261 ggtcctgcgg gaagggctgc cccttttgca tctcagtttc ttcgtggagc ccccccgcga
    10321 aatgggccgg gccggaggga tctcttccac catcatcggc accctggccc tgaccgtggt
    10381 ggccattgta atcgccaccc cgctgggcgt ggggacggcg atctttcttc gggagtacac
    10441 ccgggaaagc cggatcagcc gggtgattcg cttcggaacc gattgcctgg ccggggttcc
    10501 ctccatcatc ttcggcctct tcgggttcgt tttctttgtc atctttctag gcatggggtg
    10561 gtccatcctt tccggaggac tcacgctggc tgcgatgatt ctccccacca tcatctgcac
    10621 ggcggaagag gccatcaaat ccatccccta ttccttccgg gaggtgagtt atggtctggg
    10681 ggggacccgc tggcagacca tccggagagt ggttttgccc aacgcttttc cggggattct
    10741 gactggggtt cttctgggag tcgggcggag cgtgggggag actgcggccg tcatcctcac
    10801 cgcgggttca tctttcctga tgcccgattc catcttttct ccgatccgca ccatggctgt
    10861 ccatttctac attctggccc gggaggggat ctccatggag atggcctatg ccacgggggc
    10921 cggtctgatt attttgatcc tgatcattaa caccgtggcc aactggctcc tgttccgtta
    10981 tgtccataga ggttgagaaa agatcgagga tggaaaatca atttaaaatc gtcatccggg
    11041 attttgaggt ctattacgga tcggcgatgg ccttgaaagg cctcaacctc tccatccggc
    11101 ctagccaaat tttcgccgtt ttgggcccgg cccgaagcgg gaagaccact ttactgaaaa
    11161 gcctgaaccg cctcacggac cttatcttcg gagcgaatca tagagggcag atttttatcg
    11221 acggtcagga aatttatggg gagaacgtgt cccttccgga attacggcgg agggtgggaa
    11281 tggtcttcga ccttcccacc gccctgccca tgtcgatctt tgagaatatc gcttacgggc
    11341 cccggctcaa gggaatcgcc ggcaggaccc ggctgggaga gatcgtggag gcttcgctga
    11401 ccgcggcggt gctctgggat gaggtgaagg accgactgca cagctcagcc ttgaggcttt
    11461 cgggtgggca gcaacaaagg ttatgcatcg cccgcatgct ggctctggag ccggagatcc
    11521 ttatgcttga cgagccctgc tccggcctgg accccatctc cacggcgagc atcgaaaggt
    11581 cgttgatgag cctgaaggag aaatacacca ttctcctggt tccccacaac atccagcagg
    11641 cttcccgggt ggcggaccgg gtgggcttct tcctgggggg ggagcttatc gaagagggcc
    11701 catccgaaga aatcttcacc cggcccagaa tgaagaaaac cgaggactac atcagcggga
    11761 ggtttggata aaagcgagtt tcaagtttcg ggttgcgggt ttcgtgttca ggaaaaatgc
    11821 cgaagggcgt tgggcggatt cctttagcct cggccttctc tgcatggttt gcggctaaaa
    11881 aatgaggagg gaaaaatgga aacgcggttc cagcaggagt tgaatcattt gaaggaggac
    11941 ctcttgaaga tggccgggct ggccgagagg gccatcagca acgccattga agccctggtc
    12001 aagagggaca ccgggttggc ggagagaacc atcgccgaag acgagaagat caataaaatg
    12061 gagctgatca tcgacgaatg gtgcctgaaa ctcctggccc tccatcagcc catggccgca
    12121 gaccttcgtt ttatcacctc ggccatgagg attaacactg agctggagcg gatcggggac
    12181 ctttccgtga acatcgccga aagagtgatt tccttgaacc aggagcccca gctcaagccc
    12241 tacatcgaca ttccccggat ggcggagatc actcaaggca tggtcaagga cgtcctggat
    12301 gcctttgtga aaggagatgc cgacctggcc cggtcggttt gcgaaaggga cgatcaggtc
    12361 gatgccttaa atgaccaggt ctttcgagaa ctgctgacct acatgatgtc cgatcccaag
    12421 acgatcaccc gggcggttca tctcatcatc gtcagccgct gcctggagcg catcgcggat
    12481 cataccacca acatcgccga gggggtgatc ttcatggtca aagccctggt gatcaaacat
    12541 catgccgatg tacaggacaa accggagaaa aagagcaagg aaggaaaaaa gtgaaaaagt
    12601 aaaaaagcac cagaatccga acaaacttca acaaataaaa ttcaaattat caaaggctgc
    12661 agtttgctgg tatcattaaa acctgccgtg ataagcttca gcggcatgac gcatccgatg
    12721 agattttgac ttttcaggag tccatcaaat ttggatcatt gggaatgggt tcttggaatt
    12781 tgaaaagggg gtgcagagat gcgaaacggc ataggggttg ttttgatctt ggcttttttc
    12841 ttctgtcctt ggatgacgca ggcccaaaca caggaatcga cgccagcgca gaagaagatc
    12901 atgatggagc ggggggagaa ggtcaccggc aagcggctcg aactggtcaa aaaggtgatc
    12961 gaaagctacc gggactgcga agtccttgcc aaggggaatg aattgaccgg tttgcggatc
    13021 aacgggaagc tctactcgga cttcaccctg gtggtccgcg acgccaagcg gaccatcatc
    13081 gtggcgaccc ccgatggagt ggtcagcttt gattattaag attggggagg ggcctgctcc
    13141 cgagccgccc gtccctccac aattgaggaa cccccggttg atcttctatt tcttgcttct
    13201 ctggcactct tcatatcctt tcaccaggtt gaccaaacct cgatcaaaga agaggatcac
    13261 tcgattgttc aactccagag cccggagcaa ttcataggcc gtgtcgaaga agccggagtg
    13321 gaggataaag agccagaggt ggcggcgaaa gaggatgaaa gaataaacca gctcggaaag
    13381 aggaaagcct tcctggcagc ggtcttgtcc gtatttcttg tagtatttgg aaatctgttc
    13441 tttgggggtc tggacactta gaaattggct taacccttta tacatgctcg tggcccgctg
    13501 gtatagatgg tcttcatcga aggtgtgata gaaaggggtt agggcgtttt tccggacatc
    13561 gtttaaccat tctttggcta ttttctccgc gttcttttcg atcaattcca ccaaggcttg
    13621 agaaagcatc ttttcctcct tacccgatca atctttgatc gtcgtctggg gttgcccctg
    13681 aaaggatcct tttcaggttc aatcggtagg ggctctgcac gatccctctc tcggtaataa
    13741 ttgctgaaat aaaacgatgg ggagtcacat caaaagccgg gttatagact tttaccgcat
    13801 ggggagccat tcgctttccc cgaagggtcg taacttcggt cgaagccctc tcttcgatgg
    13861 gaattttttc ccccctggcc gtcgataaat cgatggtgga aatgggggcg gccacgtaga
    13921 agggaagccg gtgggcgcgg cagagaaggg ccacggagta agtcccgatc ttgttggctg
    13981 catcaccgtt ggccgcaatc cggtccgccc cggtgatggc cagatgaatc tttccctggg
    14041 ccatgagcca gccggccata ttgtcggcga tcagggtgaa gggaatccgg tctctcatca
    14101 gctcccaggc ggtcaaacgg gccccctgga gaagaggtcg ggtttcaccc acgtaaacat
    14161 gaatccgctt gcccgcctcc cgcgccgcgc gaatcactcc caaggcggtc ccgtagcccg
    14221 ccgtggccag ggccccggca ttgcaatagg tgagaacgga aacgccgtcg ggaatcagaa
    14281 cctgtccctg cctgcccatc tcccggttgg cttccaggtc ttcctccagg attcgaaggg
    14341 cttctttttc cagctcttcc cgaagaattt gggaatcccc gcccattttt ttaaagacca
    14401 acctgcgcat ccgctccact gcccagacta agttgacggc ggtcggccgg gtggagacca
    14461 gggcctgggc gactctgtcc acgtgcttct tcaatgcagc ccgagtcccg gatttgaact
    14521 ggcgtattcc caacaccatg ccaaaagccg ctgcaactcc gatggccggg gctccccgga
    14581 cgaccatctt ccggatagcc cgcgcggcat cctggtaggt cgagcaggcc acatacgcct
    14641 cactcagagg aagcttggtt tgatccagaa gatagagctt gccctttttc cactccaagg
    14701 tcttgattcc catggaccga acttaccata ttaaagagaa tttcatcaag aaaaaacccg
    14761 atgggttcga cccctggagt gatttcgctt ccgggcgaaa tttcagggga aatttctttg
    14821 ccttgcctca tgaggtccaa tggtcttcag ggttccagag aaaagaggag gcctttctcc
    14881 gaaatgatgt tcagctgaat tccccgaggg aaatagaacg tgtggagagg cgtatgataa
    14941 agcggttgac tacctggatg ggagccaata tattggtttc atgaactgat cgaccacttt
    15001 tctattatgg gggtactatg gccggccatc ccattgatta ttacgtgtac ggtggttcct
    15061 ggggaaccga tgaggggcga aagatttttt ccgaagaaca gatgatcgat cgctggctaa
    15121 agatcgaagc tgccctggct gaggccgaat cggagatggg aatcatcccc gagtgggcgg
    15181 ccaaggaaat ttccgccaag gcttccgtgg aaagaatcga cctgaacctg gccagggctg
    15241 aacagcagaa gacccggcac agcctggtcc cggtgatccg ggcttttgcc agggcatgcg
    15301 gagaaaaggc gggcgagttc gttcactatg ggcccaccac ccaggacatc atcgacacgg
    15361 gcaatgcgct ggctcttcgg gaggtcatgg acgcggtgaa acgggacctg gaagccatcg
    15421 agaagcgact tgtccagatc accctggaat acgcggacac tcccatggca ggaaggaccc
    15481 acggacagca tgccttgccc ataaccttcg gcttcaagac tgccagttgg ctccaagaag
    15541 tcagacgcca cttggaccgc ctggaggagg ggagaatacg cattctggtg ggagagttgg
    15601 ccggcgcggt gggaagttac gcctcctggg gggagcaagg ccgggagctc gagaggagga
    15661 cggtggccag gctgggcctg ggtcaatcgg acatcccctg gcagagctcg agggatcgtt
    15721 ccgcggaagc ggtcctcctt ctggccatga tctgttccac cctgggaaag atcgccgggg
    15781 aagtctacct gttgcagaaa acagaagtgg atgaattggc ccaggggttt gagatgggtc
    15841 gggtgggtag cagcaccatg ccccacaagc gaaatccgga gaccgccgag ggaatcaagg
    15901 gaatctcccg tctggtgcgc taccgggccg cggggatcct ggaggccatg gaaggggagc
    15961 atgaacggga tgccagtaat tggaagaccg aatgggtgat cattcccgag gtcagtatct
    16021 acactctggc cgcgctgtcc aaaacccgag agcttctcgg cgccctggca gtgaaacggg
    16081 aaaacatgag ggccaatctc gaccgaaccc ggggtttgat cctctccgag cgggccatgt
    16141 tcgtcctggg agagcacctg ggaaagcaga cagcacatga agttgtgtat gaggcctcga
    16201 tgaaggcctt tgaaacgggg aaggatttga ccagtgccct ggcggcggac tcgcgagtcg
    16261 ggaaagtctt gagcgaggag gcgatccgca agttcatgga cccggttacc tacaccggcc
    16321 tttgcgccca gctggcaagg gaagtcgctt caagggaaag aaagaaaaag ggataaaagg
    16381 gtgatcaacg aatttgccag ggagatgccc gaccaatcat gacctccagc agagccggag
    16441 gtatga
//
LOCUS       DASPRR010000153        10631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1328704, whole genome shotgun sequence.
ACCESSION   DASPRR010000153 DASPRR010000000
VERSION     DASPRR010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10631)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10631
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328704"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="secF"
                     /locus_tag="VEL68_05850"
     CDS             complement(<1..864)
                     /gene="secF"
                     /locus_tag="VEL68_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXZ35523.1"
                     /translation="MGIELLGKTNIDFIGKRKYAFILSAILVSLGVFSLVQMWRGQAN
                     LGIDFAGGTSVQLKFEKPFQMDKARHALDVNGFKDSELQQFTEGNKLLIQIKKSEAEG
                     KVADKIVAIFTKEFADNKFLVDSTMEIGPKVGKSLRDDALIAVIVSAIGMIVYIAWRF
                     EFSFGVAACIATFHDVLAVLGIFYILGKEVNLLLITALLTLAGYSLTDTVVVYDRIRE
                     NLRLRPKDFLGDVINSSINEVLSRTIVTSSTVFLVVVALFFFGGEVIHDFSFALLIGV
                     VVGTYSSVFVAS"
     gene            complement(882..2483)
                     /gene="secD"
                     /locus_tag="VEL68_05855"
     CDS             complement(882..2483)
                     /gene="secD"
                     /locus_tag="VEL68_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXZ35524.1"
                     /translation="MWRSIQLRAGLIVAFLALSIIFLVPTLVDSLPKWWTKILPSEKI
                     NLGLDLRGGMHLLLEVETQKAVESALDKYASEIKDALAKKDIPTRQVERTPDGRISIT
                     LPDAKANDRFSQVRADQFGNLKVASFREQEGKFLYLLEMNPKEAKAIEESAVRQGLET
                     IRNRVDQFGVAEPVIIPQGDKEILVQLPGIKEPQRAIELIGRTALLEFKLVDEENSLE
                     DALKGNIPPGDEVLYQKVAEKDTGVVTRRPYLLKKRALMTGDVLTDARMRIKSDFNEP
                     YVSLDFDNRGARLFDQITAENVKKRLAIVLDNNVYSAPVIQERISGGKAQISGSFTPE
                     EASDLSIVLRAGALPAPVKIIQNVTVGPSLGQDSIDKGIRAALIGAALVVVFMAVYYG
                     LSGLIADWALFLNIVYLMGAMASMRATLTLPGIAGIILAIGMAVDSNVLMFERIREEL
                     RLGKTARAAVDSGYDKALFTIIDSHVTTLITAVVLFQFGTGPIKGFAVTLSLGVIINL
                     FSALIGTKVIFDWINLKKQLKTLSI"
     gene            complement(2553..2867)
                     /gene="yajC"
                     /locus_tag="VEL68_05860"
     CDS             complement(2553..2867)
                     /gene="yajC"
                     /locus_tag="VEL68_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXZ35525.1"
                     /translation="MFSSEAYAMGGLGGGSQGGLTAFLPLILMFVVFYFLLIWPQQKK
                     AKAHKQVLANLQKGDNVVTSSGIYGSITGITDTVVTLEIAEKVRIKVARNSIAGVIKG
                     QV"
     gene            complement(3009..4217)
                     /gene="tgt"
                     /locus_tag="VEL68_05865"
     CDS             complement(3009..4217)
                     /gene="tgt"
                     /locus_tag="VEL68_05865"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXZ35526.1"
                     /translation="MKFEIIKKDSTSDARLGRYHTSHGIFHTPAFMPVGTQATVKALT
                     PEDLREIGVEIILSNTYHLFLRPGHERIQRLGGLHRFMHWDHPLLTDSGGFQVYSLNS
                     LVKISEEGVQFQSHIDGSRHLLSPEKAIEIQEAMGADIIMSLDECTPYPASHGEAERS
                     LELTLNWSRRCKDSRRREDQALFGIVQGGMYSDLRKRSVGALREMGFDGYALGGLSVG
                     ESKEMMLEVVSQTAPLLPWDRPRYLMGVGTPFDMAQAVLRGVDMFDCVLPTRNARNGM
                     LFTRSGRLVIKNARYADDDSSIDPGCSCYTCRHYSRAYLRHLYLAEEILAMRLNTIHN
                     LHFYLTFIGEIRQAMAEERLKDWVKTCYAQWNSEEENNAGQPDRWGSSSLKKTPGKRN
                     PSPARSASMK"
     gene            complement(4292..5356)
                     /gene="queA"
                     /locus_tag="VEL68_05870"
     CDS             complement(4292..5356)
                     /gene="queA"
                     /locus_tag="VEL68_05870"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXZ35527.1"
                     /translation="MKLSDFDYSLPSFLIAQVPPARRGNSRLMVLDRKKAAWEHRSFA
                     HLPDYLNSGDILVVNDTKVLPARLIGRKESGGKVEVLLVRKHGNASEERHSQEWECLA
                     QGSGRLREKTRVLFEEDVAGEFRGKTPEGLWRFCLIQGKGEDLGISLGKIGYAPLPPY
                     IHRNGDGAMRDLDLERYQTVYAQREGAIAAPTAGLHFTEETLDQIRGKGVGLASLTLH
                     VGMGTFLPVKSEEVEKHRLEREFFDLQPGTAEAINGARKPGGRAFAVGTTVTRALESS
                     ADEDGEVRARQGKTDLFILPGHQFRAVDALVTNFHLPRSTLLMLVSAFAGREFILAAY
                     EEAIKQRYRFYSYGDAMLIL"
     gene            complement(5353..6447)
                     /locus_tag="VEL68_05875"
     CDS             complement(5353..6447)
                     /locus_tag="VEL68_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HXZ35528.1"
                     /translation="MLSWVTPLPSIEAGDSIRVLILRDISSVRVASEGMSLYDLKTGQ
                     PFFKNRKYTSLVFEREEGRRVRLRGQNLSASGFLVEWTGNPLFINGRSYRDQVRVLPG
                     PNRDLWVINQLPLEEYLVGLINFEISSQWPMEAIKAQVIAARTFAIYQRRNRSGEAYD
                     VDSGVSDQVYGGIGKEDSRSRRAVEETRGELVHDQGSPVFSVYSACCGGKTESAEYMW
                     AGVFPHLRSVECTYCLDSPHFLWNYSIEGNQLAAALGIASLSRVVEMDIEERSPSRRV
                     LRLTLRGEGSQQHVSGKDFRRLLGYDQLRSTNFIVKEKDGTFLFSGLGWGHGVGFCQW
                     GAKGMAEAGADYRTILKYYYHDVEVGKTKR"
     gene            complement(6601..7929)
                     /locus_tag="VEL68_05880"
     CDS             complement(6601..7929)
                     /locus_tag="VEL68_05880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ35529.1"
                     /translation="MFGLHRLVLFLSLFCFFLPAAGWGGVAQDTLPNGLRVVLIKNPG
                     TTSVSVDLWVRAGSRFESPPINGISHFVEHLLFKGTSRRTAQEISREIAAAGGSFNAY
                     TQWEYTQLHISILPSHLELALDILSDLAQNSLMTEEMVAKERKVILEEISLGKIYPPS
                     YILNLVSKTLFADNTLEMPISGTEDSVKAIRRQDLLKFYQRQYVPNNAFLTVVGNIDG
                     SSLHGLIRQKFGAWPGRNDPPSSPPAPVRQSQFREVRERKFLDQAIVILALQAMGARD
                     IDRPSFEIINAILGSGGNSRLYQEIREKRALSYLVGSIYHPLSDTGFWGVYVGTDPKN
                     TPLVKSIISQEMARIQEEPISARELNDIKNYIRGRTLIRNENNASLAEFVSQGVLTGQ
                     WLLPEEYLGKIQAVTADDVQRVARTYLREDQRNLIILQPYPGLRFFRNLL"
     gene            8199..>10631
                     /locus_tag="VEL68_05885"
     CDS             8199..>10631
                     /locus_tag="VEL68_05885"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HXZ35530.1"
                     /translation="MKESVSQHYDRLSGFLRVVALRLQILFALDFILLFAAAFMLGLL
                     GCFFPLALGETFWYIALIYSLGVMGFLLFLLLRGLRMAFPRPSMSRVAKELEAKFPHL
                     RDDVTNSLLLFPQLKGGSVPGSISSGLISAQIKKTAEKLSALRPSEVIRFQRAGRRLP
                     LLLPILLAFSAVLALEPSFLTRSLALVTNPFSALPTRETLISLEPKGSVILRGTPLRI
                     QARATGTLPERLNLALWPEAGQETRVSMNSQGEGRFSYQVVNPQVSFLYQAYNGHSAS
                     PVYTLRVVDSPDVKSVRLTLIPPDYARLPRESKEGGHIEALKGTVVNLEGQLTKEVSA
                     AEMVLNQEAALPLEIQGESFRGNWLVLYPGHYSFKVKDEFGFENPDPVRYSIRIIPDK
                     PPEVEIYQPAQDLEISGDEEVPILYMARDDFGITAVKLIYRKGEREYAINLKSPGGER
                     YLSPQSFKWEMSSLALTPGEKVTYRLGVWDNDTVSGPKVGYSRSFTLSIRDEKARAAR
                     EGEEVHQIAEALLSLLADHLEEAKDRQALAGRMEEILKRLDRNLERMQERRDRPDFEA
                     LRKNLSSLKERMPFEGRETVTQEMERMALLAEEMARQARMNELEAMAKELRNRQQRLM
                     DFLKDQKGQLTKEARDEALKELKQLTDLLRSIMEAMSRMAPQLPDEFVNSPELSGLDF
                     QDLFSDLEDLQKKLMEGDLAGALEAAQKLLQSLSEMMAALGRAGSQAARSQMDRMQGE
                     MSRQTGELDKILSEQRDILRETERIDRELRDKINEEAEKRLKDAQPLLREHLERLKQS
                     IPPEGREGIQEGE"
BASE COUNT         2304 a         3035 c         3033 g         2259 t
ORIGIN      
        1 gctggccacg aagaccgagg aataggttcc gaccactacc ccgatgagaa gagcaaagga
       61 gaagtcgtgg atcacctcgc ccccgaaaaa gaacagggcc accacgacga gaaagacggt
      121 gctggaggta acgatggtcc ggctgagcac ctcattgatg ctggaattta tcacgtcccc
      181 gaggaaatcc ttggggcgga ggcgcaagtt ctcacggatc cggtcataga cgactacggt
      241 atccgtcagg gaatacccgg ccaaagtgag gagagctgtg atgagcagca ggttcacctc
      301 tttgcctagg atgtaaaaga ttcccagaac cgctagaaca tcgtgaaacg ttgcaataca
      361 ggcagccact ccgaaagaaa actcgaatcg ccaggcgata tacacgatca tgcctatggc
      421 cgagacgatg accgcaatca gggcgtcgtc ccgaaggctc tttcctacct tgggcccgat
      481 ctccatggtg ctgtccacca ggaatttatt gtcggcgaac tctttggtga aaatggccac
      541 gatcttgtct gctacctttc cctcggcctc ggacttcttt atttgaatca ggagtttatt
      601 cccttcggtg aattgctgga gctcgctgtc cttgaagccg ttcacatcca gggcatgacg
      661 ggctttgtcc atctggaagg gtttttcgaa tttgagctgc accgaagtcc cgccggcaaa
      721 gtcgatcccc aggttggcct gtcccctcca catctgaacc agggagaaga ctcccaggct
      781 caccaaaatg gcggagagaa taaaggcgta tttccgcttg ccgatgaagt cgatattggt
      841 tttccccaga agttcgatcc ccatgggagg gttctctcct ttcagatgct cagcgtcttg
      901 agctgcttct tgaggttgat ccagtcgaag atcaccttgg tgccaatcag ggccgagaag
      961 aggttgatga tcacgcccag gctgagggtt acggcaaagc ccttgatcgg cccggtgccg
     1021 aactggaaga ggaccacggc ggtgatcagg gtggtcacat gggagtcgat gatggtgaac
     1081 agggccttgt cgtatcccga gtccaccgcc gcccgggccg tcttccccag acgcagctct
     1141 tcgcggatgc gctcgaacat gaggacgttg ctgtccacgg ccatgccgat ggccaggatg
     1201 atgccggcaa tgccgggaag ggtgagggtg gcccgcatgg aggccatcgc ccccatgagg
     1261 tatacgatgt tcaaaaagag ggcccaatcg gcgatcagtc cggaaaggcc gtaataaacc
     1321 gccatgaaga ccaccaccag agctgcgccg atgagggccg cccggattcc cttgtcgatg
     1381 gaatcctgcc ccagggaagg ccccacagtt acgttctgga tgattttcac gggcgccggg
     1441 agagccccgg cccgcaggac gatggacaag tccgaggcct cttcgggggt gaaggacccg
     1501 ctgatctggg ccttgccccc gctgatccgc tcctggatca ccggcgcgga atagacatta
     1561 ttgtccagga cgatggccaa gcgctttttc acgttttccg cggtgatctg gtcgaaaagc
     1621 cgcgctccgc ggttgtcaaa atccagggaa acatagggct cattgaaatc cgatttgatc
     1681 cgcatccggg cgtcggtgag aacgtccccg gtcataaggg cccttttctt caggaggtag
     1741 ggccggcggg tgaccactcc ggtgtccttt tccgcaacct tctggtagag gacctcgtct
     1801 ccggggggga tattcccctt gagggcatct tccaggctgt tttcctcatc caccagcttg
     1861 aactccagga gggcggtccg accgatgagc tcgatggccc gctggggctc cttgatcccg
     1921 ggcagctgaa cgaggatctc tttgtccccc tgggggatga tgaccggttc ggcaacgccg
     1981 aactggtcca cgcggttgcg gatcgtctcc agaccctggc ggacggccga ttcttcgatg
     2041 gctttcgctt ccttgggatt catctccaga aggtagagga acttcccttc ctgctctcgg
     2101 aaagaagcca cttttaaatt cccaaactgg tccgcccgga cctggctaaa gcggtcgttg
     2161 gccttggcgt ccggaagggt gatggaaatt cttccgtccg gggtccgctc cacctggcga
     2221 gtcggaatat ccttcttggc cagggcgtct ttgatttcgc tggcgtactt gtcaagggcg
     2281 ctttccaccg ccttctgggt ttccacttcc agcaggaggt gcatcccccc ccgcaggtcc
     2341 agccccaggt tgatcttttc cgagggcaaa attttcgtcc accatttggg aagggaatca
     2401 accagggtgg ggacaagaaa gataatggag agggctaaga aggcaacgat gagcccggcc
     2461 ctcaactgga tgcttctcca catgtttcgt tctcctttgg gtcttcggac ggaatccctt
     2521 ggggtagggt caattcatga atttgcccct attcaaacct ggcctttgat caccccggcg
     2581 atggagttgc gggccacttt gatccggacc ttttcggcga tctccagggt gaccacggtg
     2641 tcagtgatgc cggtgatgct tccataaatg cccgaagagg tgaccacatt gtcccctttt
     2701 tgcaggttgg ccagaacctg tttatgggcc ttggctttct tctgctgcgg ccagatgaga
     2761 aggaagtaaa agaccacaaa catgaggatc aggggaagaa aagccgtcaa tcctccctga
     2821 gatccaccac ccaatccgcc cattgcatac gcttcgctgg aaaacatgga attaccctcc
     2881 ttaaggattt agtttgaatt caaacaaaaa cttccacccc cttgtgggga aggattgagt
     2941 tgggggaagg ttcaggtcac ccccagcctt ccctcccgcg gcgaggggga ggggagatat
     3001 tggcaaggtc atttcataga ggctgatctc gccggggacg gatttctctt cccgggcgtc
     3061 ttttttagag aagaagagcc ccagcggtcc ggctgccccg cattgttttc ctcttccgag
     3121 ttccattgag catagcaagt tttcacccaa tctttcaacc gttcttcagc catggcctga
     3181 cggatttccc ctatgaaggt cagataaaag tgaaggttat gaatcgtatt caggcgcatg
     3241 gccagaatct cctccgccag gtagagatgg cgaagatagg cccggctgta atgccggcag
     3301 gtatagcaag aacatcccgg gtcgatggac gaatcgtcgt cggcataccg ggcgtttttg
     3361 atgaccaacc tcccgctgcg ggtaaagagc attccgttcc tggcgttccg ggtgggcagt
     3421 acgcagtcga acatgtccac gccccgaagg accgcctggg ccatgtcaaa tggggttccc
     3481 acgcccatca ggtaacgggg ccggtcccag gggaggaggg gcgcggtctg ggataccacc
     3541 tcgagcatca tttccttgct ttctcccacg ctcagcccgc cgagcgcata tccgtcaaag
     3601 cccatttccc ttagggcccc cacgctcctt ttccgcaggt ccgagtacat gcccccctgg
     3661 acgattccga acaaggcctg gtcctcccgg cggcgagaat ccttgcaccg ccgcgaccag
     3721 ttcagagtca gttctaaaga gcgctcggcc tccccgtggc tggcggggta gggggtacat
     3781 tcgtccaggg acatgatgat atccgccccc atggcctcct gaatttcgat ggctttctcc
     3841 ggagaaagaa ggtgtctgga cccatcgata tgagactgga actggacccc ttcttcggaa
     3901 atcttgacaa gggagttgag gctgtagacc tggaaccctc cgctgtcggt gagcagggga
     3961 tgatcccagt gcatgaaacg gtgaagccct ccgagccgct gaatcctctc gtgccccggc
     4021 cgcagaaaaa gatggtaggt attggagagg atgatctcca ctccgatttc ccgcaggtct
     4081 tccggggtca gggccttcac cgtggcctgg gtccccactg gcatgaaggc cggggtatgg
     4141 aaaattccgt gggaagtgtg gtacctcccg agccgagcat ccgaagttga atcttttttg
     4201 ataatttcaa atttcatcgg gcaaaaaagt gtcagtgtta gtgtgagtaa agaaattgga
     4261 gcacttaagc gtgctccagg caatcttggc actataagat aagcatggca tccccgtaac
     4321 tgtagaaccg gtaccgctgc tttatggctt cctcataggc cgccaggatg aactcccggc
     4381 cggcgaaggc cgaaaccagc atgaggagag tggaacgggg aagatggaaa ttcgtcacca
     4441 gtgcgtccac ggcccggaac tggtgccccg gaagaatgaa aaggtccgtc ttgccctggc
     4501 gcgcccggac ctctccgtct tcatctgccg atgattccag cgcgcgggtt acagtggtcc
     4561 ccacagcaaa ggcccttccc ccgggtttcc gggcgccatt tatggcttcg gctgttccgg
     4621 gttgcaggtc aaaaaattct cgctccaacc ggtgcttctc gacctcctcg cttttaactg
     4681 ggaggaacgt gcccatcccc acgtgcaagg ttaaggaagc gagcccaacc cctttccccc
     4741 tgatttgatc cagggtctct tcggtaaaat gcaatcccgc cgtgggcgcg gcaatggccc
     4801 cctccctttg ggcgtagaca gtctggtatc tctccaggtc caggtcccgc atcgctccat
     4861 ctccgttgcg atggatgtaa gggggaaggg gggcataacc gatcttcccc agggaaatgc
     4921 caaggtcttc acccttgcct tggatgaggc aaaacctcca caaaccctcc ggggtttttc
     4981 cccggaattc tccggccacg tcttcctcga agaggacccg cgttttttcc cgcagccttc
     5041 ccgaaccctg ggccaggcat tcccattcct gggaatggcg ttcttcggaa gcgtttccgt
     5101 gtttcctcac caggagaacc tcgaccttcc cgccgctctc ttttctgccg atcagccggg
     5161 cggggagaac cttggtgtcg ttcaccacca ggatgtctcc ggaattcaga taatccggaa
     5221 gatgggcgaa agaacggtgt tcccaggctg cctttttcct gtccaggacc atgagccgcg
     5281 agtttccccg ccgggcagga ggaacctggg cgatcagaaa ggagggaagg ctgtagtcga
     5341 aatcgctgag cttcatcgtt tggtctttcc gacttccacg tcatgataat aatatttcag
     5401 gatggtgcgg tagtctgccc cggcctcggc catgcctttc gctccccatt gacagaaacc
     5461 gaccccatgg ccccagccca gcccagagaa gagaaaagtt ccatccttct cctttacgat
     5521 gaagttggtg gaccgaagct gatcgtagcc gagaagacgg cggaaatctt tccccgagac
     5581 atgctgctgg cttccctctc cgcgcagagt gagcctcaaa acccggcggc tggggcttcg
     5641 ctcctcgatg tccatttcca ccacccggga aagcgaagcg attcccaggg ctgcggccag
     5701 ttggtttccc tcgatggagt aattccaaag aaagtgggga gagtccaggc agtaggtgca
     5761 ctccacactc cggaggtgag ggaagacccc ggcccacatg tattccgcgg attcggtttt
     5821 tcctccgcag caggccgagt aaaccgaaaa gacaggcgat ccctgatcgt gaactaattc
     5881 tccccgagtt tcctccaccg ccctgcgcga gcgggagtct tcttttccga tcccgccata
     5941 gacctggtcg ctgacccccg aatccacatc ataggcctct cccgagcggt tcctgcgctg
     6001 atagatggca aaagtccggg cggcgattac ctgggctttg atcgcctcca tgggccattg
     6061 ggaagagatc tcaaaattga tcaagcccac gaggtactct tccaagggca gctggttgat
     6121 aacccatagg tctcggttgg gaccgggcag aaccctgact tgatcccggt aggagcggcc
     6181 gttgatgaag agcggattgc ccgtccattc caccagaaac ccggaagccg aaaggttttg
     6241 gccccggagg cggacccgcc ttccttcttc ccgctcgaaa accagagagg tatattttcg
     6301 gtttttaaag aacggttgac ccgttttcag gtcgtaaaga ctcattccct cagaagccac
     6361 cctcaccgag gagatatccc gcaggatgag aacgcggatc gaatccccgg cctcgatcga
     6421 aggcagaggg gtcacccagg aaagcacgat tcccaggaaa aggcataaag tgcggggatt
     6481 tacccgttgg tctcgattct ttcggttcac ccaggagaaa gaggacaaag agaattttaa
     6541 ataatggata ataataagaa aacccttcga tgtcaattga tattcgggtt tcggcgggct
     6601 ttagaggaga ttccggaaaa accgcaaccc ggggtaaggc tgcaggatga tcaagtttcg
     6661 ctggtcttcc ctaaggtagg tccgagccac tctctgcacg tcgtccgctg tcaccgcctg
     6721 aattttacca agatattcct cgggcaataa ccactgccca gtcagaacgc cctggctcac
     6781 gaactcagcc agagaagcat tattctcgtt ccgaatgagc gtccgtcccc ggatatagtt
     6841 cttgatgtcg ttcaactccc tggcagaaat gggctcttcc tggatcctgg ccatctcctg
     6901 ggaaatgatc gattttacca agggagtatt cttaggatcg gttcccacat acacccccca
     6961 gaaaccggtg tcggagaggg gatgatagat ggaacctacc aggtaggaaa gggccctctt
     7021 ctcccggatt tcctggtaca ggcgggagtt cccgccagag cccagaatgg cattgatgat
     7081 ctcgaaagag ggccggtcta tgtctctagc ccccatggcc tgaagggcga ggatcacgat
     7141 agcttgatcc agaaattttc tctctctcac ttcccggaat tgactctggc gcacaggggc
     7201 gggcggagag gagggggggt cgtttctccc gggccaggcg ccgaattttt gccggataag
     7261 accgtggaga ctactcccat cgatattccc cacaaccgtc aggaaggcat tgttggggac
     7321 atattgacgc tgatagaatt tcagcaggtc ctgtcggcgg atggccttaa ccgaatcctc
     7381 ggtccccgag atgggcattt ccagcgtatt gtcggcgaag agagttttgc tgaccaggtt
     7441 caggatgtaa gagggggggt agatcttccc cagggaaatc tcttccagga tcacttttct
     7501 ctccttggct accatctctt cggtcatgag ggaattctgg gcgaggtcgg atagaatgtc
     7561 cagcgccagt tccagatgag aaggcaggat ggagatgtga agctgggtgt actcccactg
     7621 agtgtaggcg ttgaaggaac cccccgcagc agcgatttcc cgagaaattt cctgcgccgt
     7681 acgcctagag gttcccttga agagaagatg ttcgacgaaa tgagatatgc cgttgatggg
     7741 gggagactca aaacgcgatc ccgcgcgcac ccagaggtcc acggagaccg aggtcgttcc
     7801 ggggttcttg atcaaaacga cgcgcagccc gttgggaagg gtatcctgcg caacccctcc
     7861 ccatccagcg gcgggaagga agaaacagaa gagagaaagg aaaagaacca aacggtgaag
     7921 gccaaacatt ttttaaactc ttgaattgcg gcctgctcct gatctctgca ggggcgaccc
     7981 gcagctcggc tgagcccgtc gagagcctct gggtcgaacg actcgccgaa gttcgggtcg
     8041 ccgctacaac gggtcctact ttattttaaa ttatactacc cgaccgcctc ttgcggaaca
     8101 gggaatggac cccaaggctc ggtttatgat ctttttcatg ttgatacccc ggctcatgtg
     8161 ctatacacta ttatcctaac cgatgatggg atgcggcggt gaaagaaagc gttagccagc
     8221 attatgaccg cctgtctggg tttctcagag tcgtcgccct tcgcctgcaa atcctcttcg
     8281 ctctcgattt cattctcctc tttgccgcgg ccttcatgct gggccttctg gggtgcttct
     8341 tccccttggc tctgggggag accttctggt acatcgcgct gatttattct ctgggcgtta
     8401 tggggtttct tctgtttctc ctgctgagag gattgcgaat ggcctttccc cgtccgtcca
     8461 tgagccgggt ggccaaggaa ctggaggcca aattccccca tctgcgggac gacgtgacca
     8521 actcccttct cctcttcccg cagctgaaag ggggaagcgt cccgggctcg atctcttcgg
     8581 gattgatcag cgcccagatc aagaaaacag cggagaagct ctccgccctt cgtccctcag
     8641 aagtcatccg atttcaaagg gccggccggc gtctgcccct gctcttgccc atccttctgg
     8701 ccttctcagc ggttctggcc ctggagcctt ctttcttgac ccgctccctg gccctggtga
     8761 ccaacccttt ctccgccctg ccgacccggg aaaccctgat ttccctggag cccaagggtt
     8821 cggtgattct gcggggcacc cctctgcgga ttcaggcccg agctacagga acccttccgg
     8881 aaagactcaa cctggccctc tggccggagg ccgggcagga gacgcgggtt tccatgaatt
     8941 cccaggggga agggagattt tcctaccagg tcgtcaaccc ccaggtctcc ttcctctacc
     9001 aggcttacaa cgggcattcc gcctcgcccg tctacaccct tcgggtggtg gactcccccg
     9061 atgtaaagag tgtccgatta accctgattc ctccagatta tgcccgtctg ccgcgggaaa
     9121 gcaaggaagg aggccacatc gaagcactga agggaacggt ggttaacctg gagggacagc
     9181 taaccaagga agtgagtgcg gcggaaatgg tcttgaatca ggaggcggcc cttccgctgg
     9241 aaatccaggg agaaagtttt cgggggaact ggctggttct ctacccgggt cattattcct
     9301 tcaaggtaaa ggacgagttc gggtttgaaa atcccgaccc ggtccggtac tccatccgga
     9361 tcatccccga taaacccccg gaggtcgaaa tttatcaacc ggcccaggac ctggaaatat
     9421 ccggcgacga ggaggtcccg atcctgtaca tggctcggga cgatttcggc atcaccgccg
     9481 taaaattgat ctaccgcaag ggggaaaggg aatacgccat taacctgaaa agtcccggcg
     9541 gagaacgata tctttcgccg cagagcttca agtgggagat gagctccttg gccctgactc
     9601 ccggggagaa agtgacctac cggctgggag tatgggacaa cgacaccgtc tccggcccca
     9661 aggttggtta ttcccgctcc ttcactctct ccatccgcga cgaaaaggcc cgggcggccc
     9721 gggaaggaga agaggtccac cagattgccg aggcgcttct ttccctcctg gcggaccact
     9781 tggaggaagc caaagatcgg caggccctcg ccggcagaat ggaagagatc ctgaagcggc
     9841 tggatcgaaa cctggagaga atgcaggaga ggagggaccg ccccgacttc gaggctctga
     9901 gaaagaatct ttcttccctg aaagaaagga tgccctttga ggggagggag accgtcaccc
     9961 aggagatgga aaggatggcc ctcctggcgg aggagatggc caggcaggcg cgcatgaacg
    10021 agctcgaggc catggccaag gaactgagga accggcagca gaggctcatg gattttctga
    10081 aagaccagaa gggccagctt acgaaagagg cccgggatga agccctgaaa gagctgaagc
    10141 aactcaccga cctcctccgt tcgatcatgg aggccatgag ccggatggcc ccccaactgc
    10201 ccgacgaatt cgtcaacagc cctgagctca gcggattgga cttccaggat ttgttcagcg
    10261 acctggaaga cctccagaaa aagctcatgg agggagacct ggcgggagcc ctggaggcgg
    10321 cccagaaact cctccagtcc ctttccgaga tgatggccgc cctgggccgg gcgggctcgc
    10381 aggcggcccg gtcccagatg gaccgcatgc agggggagat gtcccggcag accggagaac
    10441 tggataagat cctttccgag cagagagaca tcctccggga gacggaaaga atcgaccggg
    10501 agcttcggga taaaataaat gaggaagcgg aaaagaggct gaaggacgcc caacccctcc
    10561 ttcgcgaaca tctggaacgc ctaaagcagt ccatccctcc agaaggaagg gaagggattc
    10621 aggaagggga a
//
LOCUS       DASPRR010000154        18127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1487276, whole genome shotgun sequence.
ACCESSION   DASPRR010000154 DASPRR010000000
VERSION     DASPRR010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18127)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18127
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487276"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VEL68_05890"
     CDS             complement(<1..863)
                     /locus_tag="VEL68_05890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXZ35531.1"
                     /translation="MGTPALRALRESFPGSEVHLLVNAKRKEVVAGNPHVDRVFPYRN
                     NLLHRLLLFFRRLPYYDTVLVFHANEDIWAILSAVRYGVCHTRQDFQDASRRVFKLDS
                     PPRHSIQKRLALVEKAGGKKAEDFRYEFSIPPNFLGWAAQHLKDRGVFPQDPIVGLQL
                     GATDLFKCWPVEFFAQTARHLRSSRRAKIYVNAASAEKELVSRFLDLAGDEGVLVTPG
                     EDLLQSAALIQRCDVFITPDTGPMHMAIGLGVPLIGLFCPTNVEDTGPLGYDKAVILR
                     KERTCQPCLNR"
     gene            complement(916..2073)
                     /locus_tag="VEL68_05895"
     CDS             complement(916..2073)
                     /locus_tag="VEL68_05895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ35532.1"
                     /translation="MKIAFVRKAYTPYGGAERYLSQLMEKLWLQGHEVHVFAHRWEAG
                     QAGETQAEKGRPVFHRVPMISSPSFLEALSFAWSSQRLLQEQTFDIIHSFERTLYQDI
                     YRAGDGCHREWLIQRRKLDPWLKRNTYALNPLHRTLLYLEKGLFQSPRLKKIIANSRR
                     GMEEIRRHYGVPAEKIEVVHNGVDLEAFHPSNVTLYRKSLRKEFRVPAETLVILFLGS
                     GFRRKGLRGLLASLPMVKKEIPQVVLMVAGRDRVQEYREQARRLGCERNVLFLGPTQR
                     AKEFYATCDLFALPTIYDPFSNACLEAMASGIPVLTTPTNGVAELIEDGRNGFIVQDP
                     LDPTEIGRRVLEFFSSADKTGIGERARKTVLPLDLDSVVQRMLQTYEEIRK"
     gene            complement(2070..2912)
                     /locus_tag="VEL68_05900"
     CDS             complement(2070..2912)
                     /locus_tag="VEL68_05900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXZ35533.1"
                     /translation="MRAIPVFMYHHVNWHEGDLVTLSPPGFETHLRTLKDRGYQTLFL
                     DELVPILRGETVAPLPSIALTFDDGHLDNWVFAFPLLQKYGMKATIFVITSWMGEGKV
                     RGQWNPGELPGAGLPPILRHREVKKKAAQGDGTVALNWNELRAMEASGRVDIQSHTHF
                     HRDYFSAGQGVFKLSPESRGLLREDLVKSKELIETRLNKKCRYLSWPWGRYDSEAQSL
                     AESLGFEALVTTEKGVNFPGAGVGAIKRIVAKSGEESWFSRRLSIYSRRTLGHIYSRI
                     SGKI"
     gene            complement(2909..4006)
                     /locus_tag="VEL68_05905"
     CDS             complement(2909..4006)
                     /locus_tag="VEL68_05905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ35534.1"
                     /translation="MITILHTESSLGWGGQEKRTVRELLGLSCDSFRPILACQPESRI
                     GDETRKRQLRVETVRMRGNFDPLAVARLLWMIHHYSVDIVHTHSSADSWMASTAAKIS
                     PRRPKVVRTRHLNASFNIRLIYSFMADRVVTVGGSTRQYMIREKGIPAEKVMTIPTGI
                     DLSVFNADGDQGDLRQELGIPSQAPVFGTVSVFRRLKGHQVILEATPKILRAIPEARL
                     LLAGEGPQERNIRNKIEELGIGRSVLMPGFREDVPRVLNTMDVFVFPSLQEALGTAIL
                     EALAMRRAVVASRVGGIPEIIEDGKTGFFINPENPTDVAEKVIRLLKNPELRRQVGDQ
                     GRRFVEAHYDHRLMVRRLENLYRELEERRRS"
     gene            complement(4003..5109)
                     /gene="rfaQ"
                     /locus_tag="VEL68_05910"
     CDS             complement(4003..5109)
                     /gene="rfaQ"
                     /locus_tag="VEL68_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HXZ35535.1"
                     /translation="MKSLNLQNPPVRKILLIRLRNIGDVLLLVPTLRAFREAFPQAHL
                     AVAVNAGTEEMLTGNPLLDEILVFDPRWKRLPFRERVAQEGKFVWEVRKRGFDLAINT
                     TEGDRGAFLCLSTGARIKVGLYDASGLWWKGRVYDHLVRIPDWKSHLVEQMLEIPRSL
                     GLSPQDKRVEIFYPPGEREFIDRLLEKEGIGPGEDVVHIHPTSRWLFKCWRDEAMARV
                     IDQLEEKRNIRTVLTSGKEEKELKKIDHILHACRTHPVSLAGKVTLKQLAALSARARL
                     FLGVDTAPMHIAAAVGTPVIALFGPSGEFNWGPWGNRHVVIKKDWDCRPCGQDGCQGS
                     KRSRCLEEISEEEVLEEAFRLLAEPRKRPLGGIP"
     gene            complement(5096..5851)
                     /locus_tag="VEL68_05915"
     CDS             complement(5096..5851)
                     /locus_tag="VEL68_05915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ35536.1"
                     /translation="MEKISVAIITLNEEGNIRDCLESVKWADEIVVVDNGSTDGTIKV
                     CQEYRARVYQESWKGFSAQKNSAIEKTTNEWVLSLDADERIGPVLRQEIENTLQEDPP
                     VDGYWIARKNYFLGRWIRYCGWFPDRNLRLFRKSRGRFTERAVHEKVEVQGKTANLNH
                     PLIHETYRSLSDFLIRSDRYSTLAAGEMWRQGRKYRWGDVFFRPPLTFLQMYLLRAGF
                     LEGYWGFLLSVLYSYYTFAKYAKLKELGQREKP"
     gene            complement(6000..6194)
                     /locus_tag="VEL68_05920"
     CDS             complement(6000..6194)
                     /locus_tag="VEL68_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HXZ35537.1"
                     /translation="MPLSKDLLEILACPKCVRDPGCTGALEYREAQNQLVCHHCKLIY
                     RIEDDIPIMLIDEALPLENK"
     gene            complement(6267..7415)
                     /gene="waaC"
                     /locus_tag="VEL68_05925"
     CDS             complement(6267..7415)
                     /gene="waaC"
                     /locus_tag="VEL68_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HXZ35538.1"
                     /translation="MSSSVEDIPRSILIVKLSAIGDVIQTLPMLEALKRQYPRAHIDW
                     VVEEDASSLLLNHPRVNRVIISRRKSWLKKVLKPGEFWKTLREISRFVRELRSRHYDW
                     IIDNHGIFKSGLVMLLSRGRRKVGFQASAGIADEGSYFFTHERYKPLSIERHALERYL
                     DLVSQMGVQIEPTAFHFPVPPDSLKQAEALLRQKGFGSRPMVVLHPAAKWETKQWPAE
                     KFARLISALARRKATVVITGSPQDEGPVGEILHRTGGDSAKVLNLVGKTNLMELAGIF
                     SLSDLVLSPDTGPMHLAAALKAPLIALFGPTAPWRTGPYGNNPRIIRKGLPCSPCFKK
                     KCQTMECMNSISVEEVLAAVGQKLKEKGIMIADGPGGPGQSENPGFLG"
     gene            complement(7412..7993)
                     /locus_tag="VEL68_05930"
     CDS             complement(7412..7993)
                     /locus_tag="VEL68_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXZ35539.1"
                     /translation="MKKNRVVFLDRDGTLCEEVGYLDSVEQMRLIPRSGMAVRLLNQK
                     GYKVVVITNQAGVARGFFPEAVLEGLHAEMLRQLREEGAYLDGIYYCPHHPSDGFPPY
                     RQECNCRKPATGLLERAAADLNLDLLSGYMIGDHFSDVECGQRVGAQTVLLLTGHGQE
                     ALAKRESWPQPPSFIAADLFEAVLWVLGKPERA"
     gene            complement(8076..9134)
                     /gene="waaF"
                     /locus_tag="VEL68_05935"
     CDS             complement(8076..9134)
                     /gene="waaF"
                     /locus_tag="VEL68_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HXZ35540.1"
                     /translation="MGEKPVNPFAGMKRILIRSPNWVGDAVMSLPAVSSVCGKVPEAE
                     VVILAKPWVGDLFREHPRIRKVILYHSPGVHEGIWGRWRLSRELKPERFDLALHLPNS
                     IESVLISFLAGIPRRAGYDTDGRGVFLTHRVPVNGSVKKEHQVEYYLHLIRSLGIETL
                     ERIPSLQISQGRKQEAEEILTSVSRGSGPFVGISPGAQYGSAKEWFPERFGELALRIS
                     GEIGARLLILGSAGDRAAASRIGEIAGSGVIDLAGKTTLAQALALISRCRVFVTNDSG
                     LMHAASALAVPLVAIFGSTDPGRTGPLGRHSRVVYRPLPCAPCLKTQCPRNRECMEAI
                     TVEEVFKEVKELWRLGSE"
     gene            complement(9139..10017)
                     /locus_tag="VEL68_05940"
     CDS             complement(9139..10017)
                     /locus_tag="VEL68_05940"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXZ35541.1"
                     /translation="MELFFSAVNWIPLSWRIFFFEGLGRLFYLLDDRHRRIARRNLAL
                     AFPEKDPREREQIIRLAFSHLGRVAAEVFFIPRLGERNVQRHVFFEGMENFRRAQEKG
                     RGVIFLTAHFGNWEWMAAAFPLFSQHPCHVVFRPLDSPILDRMVERLRTSTGNGTIPK
                     QKAMGQILRLLKEGKTVGILLDQNMAWQEGVFVDFFGELACTNTGVALLALKTGAAVL
                     PVFNIREEDGRYRAVIEPEVPLIRTGDKKSEVLQNTQLFTRIIEGYIRNYPNQWLWVH
                     QRWKTRPWQATQVRGA"
     gene            complement(10053..11156)
                     /gene="lpxK"
                     /locus_tag="VEL68_05945"
     CDS             complement(10053..11156)
                     /gene="lpxK"
                     /locus_tag="VEL68_05945"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXZ35542.1"
                     /translation="MKSFLHRLISNPKENQLQKVLLAPLSLLALLYGWAVRTRIFFYR
                     KGIYRTHSLSCKVISVGNITLGGTGKTPFVILLAEMIRDKGYRTVVLSRGYKGKYAGP
                     YGVVSDGEKTLLDAVQAGDEPFLLAEKLKGVPIVVGKKRWISGRFALDRFKPQVVILD
                     DGFQHLGLKRDINILLLHSLHPFGNGRLFPRGELREPLNQMGRADVLILTKGEEDDMI
                     NNLGKRLMNFPEGVPLFRAHYRPQGVRVWGEEKGRDSLVLKGKKVLAFAGLARPFSFK
                     KTLSSLGVEVLKFEVFPDHHPYSSADLERLSGEALRSGAEAMITTEKDMARMRFFSPR
                     SLPLWTLAIGHEFQGNDRSLFAEFLFHRLDLKA"
     gene            complement(11193..12968)
                     /gene="msbA"
                     /locus_tag="VEL68_05950"
     CDS             complement(11193..12968)
                     /gene="msbA"
                     /locus_tag="VEL68_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HXZ35543.1"
                     /translation="MNLYRRLLEFVKPYWRHLAGAMVCMIFVSAATAGSAYLVKPVLD
                     EIFFKKDLSMLKWIPLAIIGIFILKGVFDYGQAFLMSYVGQKIIADLREKVYSHIQSL
                     SLSFFTKNPTGILMSRIMNDVGMVQGAVTDAVTGLLKDVFTVIGLVAVIFYRDWKLAL
                     IAMVVFPVAVYPIVKFGRKLRSYSTRSQTSMAELTTILLETITGTRIVKAFNMENYER
                     KRFAKENQRLFGIQIKSVRVRAISHPLMEFLGGVGIACIVFYGGYNVIQGRATPGTFF
                     SFLAALLMLYEPVKRLSGVNNTVQQGLAAATRIFEVLDSVPEIQDKLEARALPGVSRE
                     VEYQDVSFKYEEDWVLKNINLRIQVGEMVAFVGASGGGKTTLVNLLPRFYDVTAGRIL
                     IDGVDIRDYRVESLRAMIGIVTQQTILFNETVKDNIAYGKVGQSLEEVVKAAQAAFAD
                     GFIENLPRGYDTVIGEQGVKLSGGERQRISIARALLKNAPILILDEATSSLDSESEIE
                     VQKALEYLMEGRTTLVIAHRLSTIRKANRIVVISNGEIVEVGTHEELMEKDGEYKKLY
                     LLQFQDGAEAIPSASLEGEKPPTLY"
     gene            complement(13078..14217)
                     /gene="lpxB"
                     /locus_tag="VEL68_05955"
     CDS             complement(13078..14217)
                     /gene="lpxB"
                     /locus_tag="VEL68_05955"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXZ35544.1"
                     /translation="MASGKKNILIVAGEASGDLHGSKVVEALRQLDSQVRIVGMGGNH
                     LRRAGVEILIDSSRLAVVGITEVLGRLGDLLRAYRKLKELIRSQEIGLLILIDFPDFN
                     LSLAGVAKKAGVPVFYYISPQVWAWRSGRIQKIAQRVSRMAVIFPFEVALYQEAGLNA
                     EFVGHPLMDTLGQVENPSPPGDEGWSGNPLIALLPGSRHKEVKVLLPEMVRAAEIIAR
                     KRSEIGFLLALAPTITRKEVEDMLPSGGVRVQVVEGMTYQAIRAADLVWVASGTATLE
                     TAILGKPMVIAYQVSPLSYWVGRAMIRVGWIGLVNIVAGRTLVPELIQKEARGERIAE
                     ETSKILEDEPYRRGMIEGLAEVKQKLGAPGAAERVARIALEMIER"
     gene            complement(14190..15287)
                     /locus_tag="VEL68_05960"
     CDS             complement(14190..15287)
                     /locus_tag="VEL68_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ35545.1"
                     /translation="MPHRNMKTLKSSHKGKVRVGVVGIGYLGRFHAEKYAVIPEAQIV
                     GLADVIPQRAKEWAKKTRTEAFSSHRALLGKVDAVSVVVPTDQHYPIARDFLEAGCDV
                     LVEKPITTTLQEAEDLIRLARKKGRILQVGHVERFNPAVLAAREKVKAPIFIESHRLT
                     PFRGRGTEVDVVLDLMIHDLDLILSFVRSSARSIHAVGIPVLSEKVDIANTRVEFEGG
                     CVANITASRISFEDRRRIRIFQPDTYLTLDYGSKEITLYHRTFSPENRKFQISAEQIE
                     VEPGDALDKEIRAFLHSSLTRMPPAVSGEDGRNALDMAMRINKQIQENIRKIPSIAAF
                     YGVKYGLADPLFRGAPEKRKTRPWPRGKRTS"
     gene            complement(15269..16072)
                     /gene="lpxI"
                     /locus_tag="VEL68_05965"
     CDS             complement(15269..16072)
                     /gene="lpxI"
                     /locus_tag="VEL68_05965"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HXZ35546.1"
                     /translation="MKKAVGLIAGNGRLPFLLASGIRATGRPVVAIGHRETTEKDLEG
                     EVDSLHWVNIGELGKIIDILKMEGVQRAVFIGGISKTHFFSQPKPDERAIQVLLRLKD
                     KKDDAILRAVAEELEKEEIRVISPVPFLKGAMAEKGCWTRRKPTEREGKDLAFGWKMA
                     KKIGRLDIGQCIVVKEQMVLAVEAVEGTDETIRRGGRLGKGEVLVVKICKPTQDRRLD
                     LPVIGLTTLQILGEAGATCLAVEAGKTIIADKEELIRRADERGLCLIGI"
     gene            complement(16069..16842)
                     /gene="lpxA"
                     /locus_tag="VEL68_05970"
     CDS             complement(16069..16842)
                     /gene="lpxA"
                     /locus_tag="VEL68_05970"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXZ35547.1"
                     /translation="MEVHPTAIVHSKAQLASGVKIGPYCIVGENVQIGRDTILESHVV
                     AEGWTKIGDRCHLFPFVSVGAAPQAVRYKGEPTRLTIGNDNQVREFVTINRGTPEGGG
                     ETLLGNKNLIMAYSHIAHDCRIGNQVIMANSSTLAGHIQVEDFAIVGGLVAIHQFARV
                     GCYALIGGASGVPQDVPPYLIAAGNRAKLYGVNLVGLKRHNFPEATISALKLAYRILF
                     RSHLTLNKAMDKVEKEVPGLPEVRHLLDFLKSSKRGVCR"
     gene            complement(16983..17510)
                     /locus_tag="VEL68_05975"
     CDS             complement(16983..17510)
                     /locus_tag="VEL68_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXZ35548.1"
                     /translation="MKTIGVLLGVLGLGMLLTGLGWAEEPLKIGYVDMQRALNLCDAG
                     QEAKKAITGEVEKIQKTMEAKQKELNKMREDLERRGSVMNETVRREKEKEFQDKTREV
                     QRMQRDFEEDLRRKDREFSDKVLRDLARIAQKLAEDGKYTLILEANQPAILFISKTLD
                     LTEEVIKQANLQKGK"
     gene            complement(17582..>18127)
                     /locus_tag="VEL68_05980"
     CDS             complement(17582..>18127)
                     /locus_tag="VEL68_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXZ35549.1"
                     /translation="YIDPTQGSDNTLSMEYAGGFLGGSNEFIRYIFSSAWFATPFYSL
                     TFSARGVVGYITGDHVPLYELFRLGGIYTVRGFKTWSIGPTAANGEVIGGDKELLFNF
                     EMIYPIVKEIKLKGLIFFDAGNSWAKGDPYHLDELRTSVGFGFRWMSPMGPLRLEWGY
                     NLKPKPGESSSAWDFTMGGFF"
BASE COUNT         3817 a         5417 c         4799 g         4094 t
ORIGIN      
        1 tcccggttca gacagggctg gcaggttctt tcctttctca ggatcaccgc cttatcgtag
       61 cccagaggac cggtatcctc cacgttggtg gggcagaaaa gcccaatcag gggaactccc
      121 aggccgatgg ccatgtgcat aggtccggtg tccggggtaa taaacacatc gcagcgctgg
      181 atcagcgccg cagactgtaa gaggtcctct ccgggtgtca ccaaaacccc ctcatccccc
      241 gcaaggtcca gaaaacgact aacgagttcc ttctccgctg aagcggcgtt cacgtaaatc
      301 ttggctcggc gggaagaccg caggtgtctc gccgtttggg caaaaaactc aaccggccag
      361 cacttgaaaa ggtccgtcgc ccccagctgg agcccaacga tagggtcctg aggaaaaacc
      421 ccccgatcct tcaggtgctg cgcggcccag cctagaaagt tcggaggaat ggagaactca
      481 taccgaaagt cctccgcctt cttccctccc gctttttcca ccagtgccag acgcttctgg
      541 atgctgtgcc ggggcggaga atcaagcttg aaaaccctcc ggctagcatc ctgaaaatcc
      601 tggcgggtgt ggcaaacccc gtagcgaacc gcgctcaaga tggcccagat atcctcattg
      661 gcatggaaga ctaggaccgt gtcgtaatag ggaaggcgcc ggaagaaaag aagcagccgg
      721 tgaagaaggt tgttccggta gggaaagacc cggtccacat gagggtttcc ggccactact
      781 tctttccgtt tggcattcac caggaggtgg acctcgcttc cggggaaact ttcccgcaag
      841 gcgcggagcg ccggagtgcc catcagggtg tctcctatgg ctgtggtcga tacgaccaag
      901 aaacgcttcg gcgaattatt tcctgatttc ttcataggtc tgaagcattc gctggacgac
      961 ggaatccagg tccaagggaa ggaccgtctt tcgagccctt tctccgatcc cggttttatc
     1021 ggcagaggag aaaaattcca ggaccctccg cccgatctcg gtggggtcca aggggtcttg
     1081 tacgataaat ccattcctcc cgtcctcgat gagttccgcc acaccattcg tgggcgtggt
     1141 cagaacagga atgccgctgg ccatagcctc caggcaggca ttgctgaaag gatcatagat
     1201 cgtgggaagg gcgaagagat cgcaggtagc atagaattcc ttggccctct gcgtgggacc
     1261 cagaaagaga acgttccttt cgcagcccag ccggcgcgcc tgttcccggt attcctggac
     1321 ccggtccctt ccggcaacca ttaggacaac ttggggaatt tccttcttga ccatgggaag
     1381 ggaagcgagg agacccctca gccccttgcg gcgaaatccg gagcccagga aaagaatgac
     1441 cagggtctcg gccgggactc ggaattcctt gcgcagggat ttccggtaga gggtgacatt
     1501 gctgggatgg aaggcctcca ggtccacccc gttgtgcacc acttcaatct tctccgctgg
     1561 gactccgtaa tggcggcgga tctcctccat ccccctcctc gaattggcga tgatcttctt
     1621 cagccgcggg gattggaaca gccccttttc caggtagaga agggtgcgat ggagggggtt
     1681 caaggcgtag gtgtttcttt tgagccaagg gtccagcttt ctgcgctgaa tcagccactc
     1741 cctgtggcac ccgtccccgg cccggtaaat atcctggtac agcgtccttt cgaagctgtg
     1801 aatgatatcg aaggtctgtt cctgaaggag cctctgggaa gaccaggcaa agctgagggc
     1861 ttccaggaaa gaggggctgg aaatcatggg aacccgatga aacaccggcc tccccttctc
     1921 tgcctgggtt tcacccgctt ggcctgcttc ccaccggtga gcaaagacat gcacttcatg
     1981 cccctgcaac cagagctttt ccataagctg gctcaggtag cgctcggccc cgccgtaggg
     2041 agtgtaagct tttcgaacga aggcaatctt catatcttac cggatatccg gctgtaaata
     2101 tggccgagag tccggcgcga gtaaatgctc aggcgccggg aaaaccagct ctcttctccg
     2161 gacttggcca cgatcctctt aatggccccc actccagccc cggggaagtt gacccccttt
     2221 tccgtagtca ccagggcttc aaagcccaag ctctcggcca ggctttgggc ctcgctgtcg
     2281 taacggcccc agggccagga caaatagcgg cacttcttgt tcaaacgggt ttcaattaac
     2341 tccttggact tgaccaagtc ttccctcagg agtcctcggc tttccgggga gagcttgaat
     2401 acgccctgac cggcggaaaa ataatcccgg tgaaaatgcg tatgcgattg gatatccacc
     2461 cgcccgctcg cctccatggc gcggagttca ttccaattca gcgccacggt tccatccccc
     2521 tgggcggctt tcttcttgac ctctctgtgg cggaggatgg ggggaagacc tgcgcccgga
     2581 agctcccccg gattccactg gccccgcact ttcccttctc ccatccagga ggtgatgacg
     2641 aagatcgtag ccttcatccc atatttttgg agcaacggaa aagcaaagac ccaattgtcc
     2701 agatggccgt catcgaaggt cagcgcaatg gaaggcagcg gggcaaccgt ttctccccgg
     2761 aggatgggca ccaattcatc cagaaagagg gtctggtatc cccggtcctt cagcgtccgc
     2821 aggtgagttt cgaaccccgg gggtgagaga gtgaccaaat ccccctcgtg ccagttgacg
     2881 tggtgataca tgaaaaccgg gatggctctc atgatctgcg cctctcctcg agttcccggt
     2941 acaggttttc cagcctccga accatcagcc tgtggtcgta atgggcctcc acaaaccggc
     3001 gcccctgatc tcccacctgc cggcgtagtt ccgggttttt caggaggcgg atgactttct
     3061 cggctacgtc ggttggattt tccgggttga taaaaaatcc tgtttttccg tcttcgatga
     3121 tctccggaat tcctcccacg cgggaggcca ctactgccct tctcatagcc agggcctcca
     3181 agatggccgt tcccagcgct tcctggagag acgggaatac gaacacatcc atcgtgttca
     3241 aaacgcgcgg gacgtcctcc cggaatccag gcataaggac cgacctcccg attcccagct
     3301 cctcgatttt gttccggata ttcctctcct gaggcccttc cccggccagg agaagccggg
     3361 cctcggggat ggctcgaaga attttagggg tcgcctcgag aatgacctga tggcctttca
     3421 accggcgaaa gactgatacg gttccgaaaa cgggtgcttg ggacggaatt cccagttcct
     3481 gacgaaggtc cccctggtcc ccatccgcgt tgaacaccga caggtcgatc ccggtaggga
     3541 tagtcatgac tttttcggcc ggaattccct tctcccggat catgtactgc cgggtgctgc
     3601 cccccacggt aaccacccgg tcggccataa agctgtagat gaggcgaatg ttgaaggagg
     3661 cgtttaaatg gcgagtgcga accactttcg gccgccgggg agaaatcttg gcggcagtgg
     3721 aagccatcca gctgtcggcc gagctgtggg tatggacaat gtccaccgaa taatgatgaa
     3781 tcatccagag gagccgagcc accgccaggg gatcaaagtt tcccctcatt cttacggttt
     3841 ccacccggag ctgtctcttc cgcgtttcgt ccccgatccg gctctccggc tggcaggcca
     3901 ggatagggcg gaaagaatcg caggaaagac ccaggagttc ccgcacggtc cgtttctcct
     3961 gcccccccca tcccagggaa gactcggtgt gcaggatggt gatcaaggga tccctcccaa
     4021 gggccttttc cggggttcgg caaggaggcg aaaggcttcc tcaaggactt cttcctcgga
     4081 aatctcttcc aggcagcggc tgcgcttgct cccctggcat ccgtcttgcc cgcaggggcg
     4141 gcaatcccag tcctttttga tgactacatg gcggttcccc catgggcccc agttgaattc
     4201 tccggaaggg ccgaagaggg cgattaccgg tgttcccacg gccgccgcga tatgcatggg
     4261 ggccgtatcc accccgagga agagccgggc gcgtgcgctc agggcggcga gctgctttag
     4321 agtcaccttc cctgccaggc tgactggatg ggttcggcag gcatgcaaaa tatgatcgat
     4381 cttcttcagt tccttctctt cttttccgct cgtcaggaca gtgcggatgt ttcttttctc
     4441 ttccagttga tcgatgaccc tggccatggc ctcatcccgc cagcatttga aaagccagcg
     4501 ggaggtggga tggatatgca ccacatcttc cccgggaccg atcccctcct tctccaggag
     4561 ccgatcgatg aattcccgct ctcccggagg ataaaaaatc tccactcgct tgtcctgcgg
     4621 actcaggccc aggctgcgtg gaatctccag catttgctcc accaggtggg acttccaatc
     4681 cgggatgcgc accaggtggt cgtagaccct tcccttccac cacaatccgc tggcatcgta
     4741 aaggcccacc ttgatccggg cgccagtgct taggcatagg aaagctcccc ggtctccttc
     4801 cgtcgtgtta atggccaggt cgaatcctcg cttccggacc tcccatacga attttccttc
     4861 ttgggcgact ctctctcgga agggcagcct cttccatcgg gggtcgaaga ccaggatttc
     4921 atcgagcagg ggattgccgg tgagcatctc ttcggtcccc gcatttaccg ctacggccag
     4981 gtgggcctgg ggaaaggctt cccgaaaagc ccggagggta ggaaccagga gaaggacatc
     5041 gccgatgttc cgcagccgga tgagcaggat ctttcgaacc ggcgggttct ggaggttaag
     5101 gcttttcacg ttgtccgagc tctttcagtt tggcgtattt ggcaaaggtg tagtaggaat
     5161 agaggacgga gaggagaaag ccccaatacc cttccaagaa tccggcccgg aggagataca
     5221 tctgcaaaaa agtaagaggg gggcggaaaa atacatcccc ccaacgatat tttctcccct
     5281 ggcgccacat ttccccggcg gccagcgtgg agtagcggtc cgaccggatt aaaaaatcgc
     5341 tcagcgagcg gtaggtttca tggattagcg ggtgattcag gtttgccgtc tttccttgta
     5401 cctccacctt ttcatggacg gccctctccg tgaaacgtcc ccggcttttg cgaaaaagcc
     5461 gcaggttccg atccgggaac cagccgcaat accggatcca ccttcccaga aaatagtttt
     5521 ttcgggcaat ccaatagcca tcgaccggcg ggtcctcttg aagggtgttt tcgatctcct
     5581 gacgaaggac cggcccgatc ctttcgtccg cgtccaggct caacacccac tcatttgtcg
     5641 tcttttcaat agcactgttc ttctgggcag aaaatccctt ccagctctcc tggtaaaccc
     5701 gggcccggta ttcctggcag acctttatag tcccatcggt gctgccgttg tccaccacca
     5761 cgatttcgtc ggcccatttc acgctttcca gacagtcgcg gatgttcccc tcttcgttca
     5821 gggtgatgat ggccacgctg atcttttcca taaagaaaga cagttcacca ccgaggcaca
     5881 gagaacacgg agaaaatcta gttcaaaaat ggaaaggatt aaaacctgag gggggagtta
     5941 ataatttccg ctatattcat tatctccgtg ccctctgtgt ctctgtggtg aatctatttt
     6001 tatttgttct ccagcgggag cgcttcgtcg atgagcatga tggggatgtc atcctcaatg
     6061 cggtagatca gcttgcagtg atggcaaacc agctgattct gcgcttcccg gtactccagg
     6121 gctcccgtac agcctgggtc ccgtacgcac ttgggacagg ccagaatttc cagaaggtct
     6181 ttactcagag gcatgggttt tcctttcgat ttgttttggg gtccagattg gcgcaggaag
     6241 aacggaccgc ccgtctccct ttctgcctac cccaaaaatc ctgggttctc actttgtccg
     6301 ggtcccccgg gtccatctgc aatcatgatt cctttttcct tcagcttctg acccacggcg
     6361 gcaaggacct cctcgacgct gattgaattc atgcactcca tcgtttggca tttttttttg
     6421 aaacaggggc tgcaggggag tcccttccgg atgatccggg ggttattccc gtagggccca
     6481 gtcctccagg gagccgtggg cccgaagagg gcgatgagcg gagccttcaa cgcagccgcc
     6541 agatgcatgg gcccggtatc cgggctcagt accagatcgg agagggaaaa gataccggcc
     6601 agctccatca gattggtctt tcccaccaaa tttagaacct tggccgagtc gccccccgtt
     6661 cgatggagga tttcacccac cgggccttcg tcctgggggc taccggtgat gactaccgtg
     6721 gccttcctcc gggccaaggc agagatcagt cgggcaaact tttccgcggg ccactgtttg
     6781 gtctcccact tggctgcggg atggagcacc accatgggcc gggacccaaa acctttctgc
     6841 cgcagcaagg cttccgcttg cttcagcgag tcgggaggga cgggaaaatg gaaagctgtc
     6901 ggttcgatct gaacccccat ctgggagact aggtccagat agcgctccag ggcatgccgt
     6961 tcgatgctca gaggcttata ccgttcatgg gtgaagaaat agcttccttc atcggcgatc
     7021 cctgccgaag cctggaagcc gaccttacga cgtccccgcg ataagagcat caccaaaccg
     7081 ctcttaaaga ttccatggtt atcgatgatc cagtcgtaat gccggctcct taactcgcgg
     7141 acgaaccggg atatttcccg cagagtcttc caaaattccc ccggtttcaa gactttcttg
     7201 agccaggatt tgcgtcggct gatgatgacc cggtttaccc tgggatgatt caatagaagg
     7261 ctgctggcat cttcttccac cacccagtcg atgtgggccc ggggatactg cctcttcagg
     7321 gcctccagca tggggagggt ctgaatcaca tccccgatcg cgctgagttt tacaatcagg
     7381 atggaccggg gaatatcttc cacggaagaa ctcatgcgcg ctccggttta cccagaaccc
     7441 aaaggaccgc ttcaaagaga tcggcggcga taaaggaagg gggctggggc cagctctccc
     7501 tcttggccaa agcctcctgc ccgtggccgg ttaatagcag aaccgtttgc gcaccgaccc
     7561 gctgcccgca ctccacatcg gaaaagtggt ccccgatcat gtagcccgaa aggaggtcca
     7621 ggttcaagtc agcggctgcc ctttcgagga ggccggtggc tggcttgcgg caattgcatt
     7681 cctgacggta gggaggaaaa ccgtccgaag gatggtgggg gcagtaataa atgccgtcca
     7741 ggtaagcccc ctcttcccgc agttgacgca gcatttccgc gtggagacct tccaggacag
     7801 cctccgggaa aaaaccccgg gcgacccccg cctggttggt aatgaccact actttgtatc
     7861 ctttctggtt taagaggcgc accgccatgc ccgaccgggg aatcagcctc atctgttcca
     7921 ccgaatccag gtatcccact tcttcgcaga gggtgccgtc ccgatccaaa aaaacgactc
     7981 gattcttctt cataggaaca tctcaaatga aaaacttaac taccggtgcc tgaatacgat
     8041 ggtagcttaa ttgaaattcc gatatgggat ttttctcact cgcttcccag cctccatagt
     8101 tccttcactt ctttgaagac ttcctccacg gttatggctt ccatgcactc ccggttccgg
     8161 gggcattggg tcttcaggca gggagcgcag ggaaggggtc tatagacgac gcggctgtgc
     8221 cggcccagcg gaccggtcct cccgggatcg gtggagccaa aaatggccac caaaggaact
     8281 gccagagccg aggccgcgtg catgaggccc gagtcgttgg tgacgaaaac ccggcatcgg
     8341 gagatgagag ccaaggcctg ggcaagggtc gttttcccgg cgaggtcgat caccccggaa
     8401 cccgcaattt ccccgattcg ggaagcggcg gcccgatccc ctgcactccc caggatcaac
     8461 agccgggctc cgatctcccc cgagatgcgc agggccagct ccccgaacct ctccggaaac
     8521 cactctttcg ccgacccata ttgggccccc gggctgatgc ccacgaaagg cccgcttcct
     8581 cttgaaacgg acgtcaggat ttcttccgct tcctgtttcc ttccttggga aatctgaaga
     8641 gaaggaatcc tctccagggt ttcaatgccc aaggaccgga taaggtggag gtaatactcc
     8701 acctggtgtt ccttcttgac acttccgttt accggaaccc gatgggttaa aaagactccc
     8761 ctcccatcgg tatcgtagcc ggcccgccgg ggaatcccgg cgaggaaaga gatgaggacc
     8821 gactcaatgg aattgggtaa gtggagggcc aagtcgaatc gttccggctt cagctctcga
     8881 gaaagcctcc atcttcccca tatcccttca tggactcccg ggctgtggta aagaatcacc
     8941 ttccgaatcc ttggatgctc tcgaaacaaa tcgcccaccc agggtttagc caggattacg
     9001 acttccgcct cgggaacctt tccacagacc gatgagacgg ccggcaggct catgactgca
     9061 tcccccaccc aattggggga tcggatcaga atccttttca tcccagcaaa gggatttaca
     9121 ggcttttctc ccatgcgctt atgcacccct tacttgtgtg gcctgccagg ggcgggtttt
     9181 ccatcgctga tgaacccaca gccattggtt cgggtaattt cggatatagc cctcgatgat
     9241 ccgggtgaag agctgcgtat tctggagcac ttcggacttt ttatctcccg tccggatcaa
     9301 ggggacctcg ggctcgatga ccgcccggta gcggccatct tcttcccgaa tgttgaaaac
     9361 gggaagtacc gccgccccag tcttcagggc caacagggcc actcccgtgt tggtacaggc
     9421 cagctctcca aaaaaatcta caaagactcc ttcctgccag gccatgttct gatccagcag
     9481 gattcccacg gtcttcccct ccttcagcag gcgaagaatt tggcccatgg ccttctgctt
     9541 ggggatggtt ccatttccgg tgctggtccg cagcctttcc accatccggt ccaggatagg
     9601 actgtccaag gggcggaaga ccacgtggca gggatgctgg gagaacaggg ggaaggccgc
     9661 agccatccat tcccagtttc caaaatgggc ggtgaggaag ataactcccc tccccttctc
     9721 ttgggctcgg cggaaattct ccattccctc gaagaaaaca tggcgctgaa cgttcctttc
     9781 gcccagccga ggaataaaga aaacctccgc tgccacacgt cccaggtggc taaaggccag
     9841 tcggatgatt tgctcccttt cccgggggtc tttctccgga aaggccaagg ccaggttccg
     9901 tcgggcgatt cgtcggtggc ggtcgtcgag caggtagaaa agccgaccca gcccttcaaa
     9961 aaaaaatatc ctccaggaaa gaggaatcca atttaccgcg gaaaaaaaaa gttccatcaa
    10021 gagatcgggt ccgggccttt ctttccttcc tctcatgctt tcaagtccag ccggtggaaa
    10081 agaaattcgg caaaaaggga tcgatcattc ccctggaatt catgaccgat agccagggtc
    10141 cataagggaa gagagcgggg agaaaaaaaa cgcatccggg ccatatcttt ttctgtggtg
    10201 atcatggcct ccgcaccgga tcgcagggcc tccccggaaa gacgctccag gtctgcagag
    10261 ctataaggat ggtgatcggg aaaaacttca aatttcagga cttccacccc caggctggaa
    10321 agggtcttct tgaaggaaaa aggccgagcc agcccggcga aagctaggac tttcttcccc
    10381 tttaaaacca aagagtccct ccccttttcc tctccccata ccctcacccc ctggggccga
    10441 taatgggcac ggaaaagggg aaccccttcc ggaaaattca ttaatctttt ccccaaatta
    10501 tttatcatat catcctcttc cccctttgtc aaaatcagca catcggcccg ccccatctgg
    10561 ttcaagggct ccctcaattc tccccgcgga aacagtctcc cgttcccaaa gggatgaagg
    10621 gaatgaagaa gaagaatatt aatgtccctt ttcagcccca ggtgctgaaa cccgtcatcc
    10681 aggattacca cctggggttt gaatcgatcc agagcgaatc gtcccgaaat ccatcgcttt
    10741 ttccccacaa cgatcggaac tcctttaagc ttctcggcca gcagaaaggg ttcatctccc
    10801 gcttgaacgg catccaggag ggttttttct ccatcggaca ccaccccata aggcccagcg
    10861 tattttccct tgtagccccg ggaaaggacc actgtccggt atcccttgtc tcggatcatc
    10921 tccgccagaa gaatgacgaa gggagtcttg cccgttcccc caagggtgat attccccacg
    10981 ctgatcactt tgcaggaaag ggagtgggtt cgatagattc ctttgcggta gaaaaaaatg
    11041 cgggttctga ccgcccagcc gtaaaggagg gccaggaggg aaagaggggc aagcaggact
    11101 ttttgaagct gattttcttt gggattgctg atgaggcgat gcaagaagct tttcaatttg
    11161 gcccttgact tgtagattcg gtcttttccc cctcaataaa gcgtgggcgg tttttctcct
    11221 tcaagagaag cggaggggat tgcctcggct ccatcttgga actgcagcag gtaaagtttc
    11281 ttatattctc catccttttc catcagttct tcatgggtcc ccacctcaac aatctccccg
    11341 tttgaaatga ccacaattcg gtttgccttc cggattgtgg agagacgatg ggcgatcact
    11401 agcgtggtcc ggccctccat gaggtattcc agggccttct ggacctcgat ctcggattcg
    11461 gaatccaaag atgaagtggc ttcgtccagg atcaggatgg gggcgttttt caggagagcc
    11521 cgagcaatgc tgatgcgctg gcgttccccc ccggaaagtt ttaccccctg ttccccgatg
    11581 accgtatcat atccccgggg aaggttttcg atgaacccgt cggcgaaagc ggcctgggcc
    11641 gccttcacaa cctcttcaag cgactggcca actttcccgt aagcgatatt gtccttcacc
    11701 gtctcgttga aaaggatggt ctgctgggtg acgatgccga tcatggccct cagggactcc
    11761 acccggtaat cgcggatatc cacgccatcg atcaaaattc tgcccgcggt tacgtcgtaa
    11821 aagcggggca gcaggttgac cagcgtagtt ttacccccgc cgctggctcc cacgaaggcc
    11881 accatctctc ccacctggat gcgcaggttg atgttcttca ggacccagtc ctcctcgtac
    11941 ttgaaagaaa catcctgata ttcgacctcc cgggagacgc cgggtagagc ccgcgcttcc
    12001 agtttgtcct gaatctccgg aacactgtca agaacctcga agatccgggt tgcggcggcc
    12061 agcccctgct gcacggtatt gttgactccg ctcaatcttt tcactggttc gtagagcatg
    12121 agcagggccg ccaggaaaga aaagaaggtc cccggagtgg ccctcccttg gatcacgttg
    12181 tatcctccgt agaaaacgat gcaggcaatc cccacccccc ccaggaattc catgagggga
    12241 tgggaaatgg ccctgacccg gacggatttg atctggattc cgaagagacg ctggttttcc
    12301 ttggcgaagc gcttccgttc gtagttttcc atgttgaagg cttttacgat ccgcgttccg
    12361 gttatggttt cgagaaggat ggtcgtgagc tcggccatgg acgtctggct gcgggtgctg
    12421 taggacctca gcttccggcc aaacttgacg atgggataaa cggccaccgg aaaaacgacc
    12481 atggcgatca aggccagctt ccagtctcgg tagaagatga ccgccaccaa gccgatgacg
    12541 gtgaagacat ctttcaaaag accggtaacc gcatcggtca ccgctccttg gaccatgccc
    12601 acatcgttca tgatgcggga catcagaatt cctgtagggt tcttggtgaa aaaggaaagg
    12661 gataaagatt gaatgtggct gtagactttc tcccgaaggt cggcaatgat cttctgcccc
    12721 acataggaca tcaggaaagc ctggccgtaa tcgaaaaccc ctttcaggat gaagatcccg
    12781 atgatggcca gggggatcca tttcaacatg gacaggtctt tcttgaagaa gatttcatcc
    12841 agcaccggtt ttaccaggta ggccgaaccg gccgtcgccg cagagacgaa gatcatgcag
    12901 accatagccc cggccaggtg tctccaatag ggtttgacga attccagaag ccttcggtag
    12961 agattcataa gaacagcttt cagctatcag ctgtcagcta atagctatca atgttcttgc
    13021 atgaagtctg gtttgagcgg aggggatggc tgaaagctga tcgctgccag ctatccgcta
    13081 tctttcaatc atttccagag ctattcgggc taccctttca gccgcccctg gagcccccag
    13141 cttttgtttg acttcagcaa gcccctcgat catgcccctg cggtagggct catcctccag
    13201 gatttttgag gtttcctctg caattctctc tcccctggcc tccttttgga tgagctcggg
    13261 gaccagggtt cggccggcta caatgttcac cagtccgatc cacccgaccc gaatcatcgc
    13321 cctccccacc cagtagctga ggggggaaac ctggtaggcg atcaccatgg gtttgccgag
    13381 gatggccgtc tccagcgtag ccgttcctga agcgacccac accaggtcgg cagcccttat
    13441 ggcctggtag gtcattcctt cgaccacctg gaccctgacc ccgccggaag gcagcatatc
    13501 ttcaacctcc ttccttgtta tggtgggagc cagggccaac agaaacccga tttcagacct
    13561 cttccgggcg atgatttccg ccgcccggac catttccggc aggagaacct ttacctcctt
    13621 atgccgactc cccggcagca gggcgatcag cggatttccg ctccatccct catccccagg
    13681 aggggatgga ttttccacct gtcccagagt atccatcagg ggatgaccca caaactccgc
    13741 attgaggcca gcctcttggt agagggccac ctcgaaagga aagatcaccg ccatccggct
    13801 tacccgctgg gcaatttttt gaatccgtcc cgaacgccaa gcccaaacct gggggctgat
    13861 gtaataaaaa accggaactc ccgctttttt agcgaccccg gcaaggctca ggttgaaatc
    13921 cgggaaatca attaatatca ggagtccgat ttcttggctg cggatcagtt ccttcaactt
    13981 ccggtaggcc cggagcaaat ctccgagccg gcccaggact tcggtgatcc ccaccacggc
    14041 caaacgggaa gaatcaatca gaatttcgac tcctgcccgg cgcaggtgat tcccccccat
    14101 tcctacgatt ctgacttgtg agtccagctg ccgaagagcc tccaccactt tggagccatg
    14161 gaggtctccc gaagcctctc cggctacgat caggatgttc tttttccccg aggccaaggt
    14221 cgggtcttcc ttttttcggg ggccccgcgg aacaaagggt ccgccaggcc gtatttcact
    14281 ccgtagaatg ctgcaatgga ggggattttc cggatgtttt cctgaatctg cttgttgatt
    14341 ctcatggcca tgtccagggc atttcttccg tcctccccgg atactgccgg aggcatccgg
    14401 gtcaaagagg aatggaggaa ggctcgaatt tccttatcta aggcatcccc cggttccact
    14461 tcaatttgct cggccgaaat ctgaaatttc cggttctctg ggctgaaagt ccggtggtac
    14521 agagtgatct ccttggagcc ataatccagg gtcaggtagg tgtccggctg gaaaatccgg
    14581 atgcgccgcc ggtcctcgaa ggaaatccga ctggcggtga tattggccac gcatccgccc
    14641 tcaaattcca cccgggtgtt cgcgatgtcc accttctccg agagcacggg aatgccaacg
    14701 gcatggatac tcctggccga tgaacggaca aaactcagga ttaaatccag gtcgtgaatc
    14761 atcaggtcca ggaccacatc gacctccgtc ccccggccgc ggaaaggggt cagccggtgg
    14821 gattcgataa agatgggggc cttgactttt tcccgggcgg cgaggacggc ggggttaaac
    14881 cgttccacat gtcccacctg caggattctg cccttctttc tggccaggcg gatcaaatct
    14941 tccgcctcct gcagtgtggt ggtaatcggt ttttctacca gcacgtcaca tccggcctcc
    15001 aaaaagtccc gggctatggg gtaatgttga tcggtgggca caactacgct caccgcatcc
    15061 accttcccca gaagggcccg gtgactggaa aaggcttcgg tccgcgtctt cttcgcccac
    15121 tccttggccc tttggggaat cacgtcagcc aaccccacga tttgcgcctc ggggataact
    15181 gcgtatttct ccgcgtggaa ccggccgagg tacccgatcc ctacgactcc cacccggact
    15241 tttcctttat gggaactttt gagggttttc atattccgat gaggcataat cccctctcgt
    15301 ccgcccggcg gatcaattcc tccttgtccg ctataatggt ctttcccgct tccactgcca
    15361 ggcaggtagc cccggcttcg cccagaatct gcagggtggt gaggccgatc accggaaggt
    15421 ccagccggcg gtcctgagtg ggtttgcata tcttcacgac caagacctcg ccttttccca
    15481 accggccccc ccgccggatg gtctcgtccg ttccctcgac ggcctccacg gccagaacca
    15541 tctgttcctt gacaactatg cactggccga tatccaggcg gccgatcttc ttggccatct
    15601 tccagccgaa ggcaaggtct tttccctccc gttcagtagg cttccgtcga gtccagcatc
    15661 ccttttccgc catagcgccc ttcagaaaag ggacagggct gatcacccga atttcctcct
    15721 tttccaattc ctccgccaca gccctcagaa tggcgtcatc tttcttgtcc tttaaccgaa
    15781 gcaggacctg aatcgcccgc tcatcgggct tgggctggga aaagaaatgg gtcttggaaa
    15841 tccctccgat gaaaaccgcc cgttggaccc cttccatttt cagaatatcg atgatcttgc
    15901 ccagttctcc gatgttcacc caatgcaggg aatcgacctc cccttccaaa tccttctccg
    15961 tcgtctcccg atgaccgatg gctaccaccg ggcgtcccgt ggcccggatc ccggaagcca
    16021 gtagaaaggg aagccttccg ttcccggcga tcaatccgac ggcttttttc atcgacagac
    16081 gccccgcttg gagcttttaa gaaaatcgag caggtgtcgc acctccggaa ggccgggaac
    16141 ctctttctcc accttatcca tggctttgtt cagggttaag tgagagcgga agagaatccg
    16201 gtaggccaat ttgagagccg agatcgtggc ctcgggaaag ttatggcgct tcagcccaac
    16261 gagattcacg ccgtaaagct tggcccggtt tcccgcggcg atcaggtagg gaggaacatc
    16321 ctggggaacc cccgaggccc cgccaatgag ggcgtagcat cccacccggg caaactgatg
    16381 gatggccacc aaccctccca cgatggcaaa gtcttccacc tggatgtgcc cggccagggt
    16441 tgaactgttg gccataatca cctggtttcc gatcctgcaa tcatgggcaa tgtgggagta
    16501 ggccatgatc aagttcttgt tcccgagcaa ggtctcccct ccgccttcgg gcgtcccccg
    16561 gttgatggtc acaaactccc gtacctggtt atcgttcccg atggtcaacc gggtcggctc
    16621 gcccttgtat cgcacggcct gaggagcggc ccccacggaa acaaagggga agagatggca
    16681 ccgatccccg attttcgtcc acccttcggc caccacatga gactccagga tggtatctct
    16741 cccgatttgt acattttccc cgacgataca gtagggcccg atcttgaccc cggaggccag
    16801 ttgggctttg gagtggacaa tggctgttgg atggacttcc aattgggtct ccttattagg
    16861 atggcaatcc tcgtttcgct ctaaacggtc agcaaatacg cttttcagaa aaaacaataa
    16921 atcattcgaa ctctagtctc ttcctgatcg ctgatagctg attccatcct gagggcctgg
    16981 gtctatttgc ccttctgcaa attggcctgt ttgatcactt cttcggtcag gtccagggtc
    17041 ttggatatga agaggatcgc gggctgattg gcctccagaa tcaatgtata ttttccatct
    17101 tcggccaact tctgggcgat cctggccagg tcccgcaaaa ctttatccga aaattcccga
    17161 tcctttcggc gaaggtcctc ctcgaagtcc cgttgcattc tctggacctc ccgggtcttg
    17221 tcctggaatt ccttttcttt ttcccggcgc acggtctcat tcatgaccga gccgcgtctc
    17281 tccaggtcct cccgcatctt attgagctct ttctgcttag cctccatcgt tttctgtatc
    17341 ttttccactt ccccggtgat ggcctttttc gcttcctggc ccgcatcaca tagattcaaa
    17401 gctcgctgca tgtccacata cccgattttt aagggctcct ccgcccagcc caagccagtc
    17461 agaagcattc ccaagcccaa gacgcccaaa agaaccccga tcgttttcat ccttttctcc
    17521 ttaaagattg tttttctttc atgacccccc cattgtaacg cagatctctg actctgtgaa
    17581 tttagaagaa cccccccatg gtgaaatccc aggcgctcga cgattctcct ggtttgggct
    17641 ttaggttgta tccccattcc agccgcaggg ggcccatcgg ggacatccat cggaagccga
    17701 atcccacact cgttcgcagc tcatccagat ggtatgggtc ccctttggcc caggaattgc
    17761 ccgcatcgaa gaagatcagt cccttgagtt tgatctcctt gacgatggga tagatcattt
    17821 caaagttgaa gagaagctcc ttgtcgcccc cgatgacttc cccattggcg gcggtggggc
    17881 cgatgctcca ggttttgaaa ccccgcaccg tgtagattcc tcccagccga aacagctcat
    17941 atagggggac gtgatccccg gtaatgtacc cgaccacccc ccgggcggaa aaggtaagac
    18001 tgtaaaaggg ggttgcgaac caggccgaac taaagatata tcgaatgaat tcgttgcttc
    18061 ccccgagaaa cccgccggcg tactccatgg aaagagtgtt atccgatccc tgggtgggat
    18121 caatgta
//
LOCUS       DASPRR010000155        40254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1005694, whole genome shotgun sequence.
ACCESSION   DASPRR010000155 DASPRR010000000
VERSION     DASPRR010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 40254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40254)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40254
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005694"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1011
                     /locus_tag="VEL68_05985"
     CDS             277..1011
                     /locus_tag="VEL68_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXZ35550.1"
                     /translation="MNGAKRNFSNKLLLPILSSVFLLVWSNGCALFGKKEPATTPQAA
                     YDEGVLLLEKKKYERSAEAFRKFKEDYPLSTYTPLAELRLGDSLYFDKNYAEAIVQYE
                     EFKKLHPVHAEIPHAIYQIGMCYFKQMLSIDRDQTVTEKALEQFRYVVENFPQSKYLP
                     DAKTKMQLCQRQLADQEFDIGYFYYRMGYYKAALGRFEEILKKFPDAGLERKITPLMK
                     TCREKIAKEEKKLKEKEGKEAKKKQS"
     gene            complement(1085..3730)
                     /gene="alaS"
                     /locus_tag="VEL68_05990"
     CDS             complement(1085..3730)
                     /gene="alaS"
                     /locus_tag="VEL68_05990"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXZ35551.1"
                     /translation="MKGSAIRQKFLNYFKSQGHTIVSSSPLVPQKDPTLLFTNAGMVQ
                     FKNFFTGEEKAPFPRATTSQKCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFK
                     EGAIAMAWEFLVDLLGLPPDKLWATIHEGEKSLGIGPDQEARQIWKKYLPEERIRTFP
                     SKDNFWQMGDTGPCGPCSEIVIDQGPGVGCGRRECALGCECDRYLELWNLVFMQFERF
                     ADGTIKPLPKPSIDTGMGLERITAVVQGVRSNYDTDLFGRILKAIGGLSGKKYDPSSP
                     EGVSFRVIGDHARAATFLISDGVLPSNEGRGYVLRRIMRRALRHGRKLGINEPFLFRL
                     TDDVIALMEDAYPELASHREMVKNMISNEEDRFSETLNTGLRLWADEIQNLKDRESKV
                     IPGELVFKLYDTYGFPLDLTEEIARDEGFSLDKKGFEDAMETQRERARESWKGSGEEA
                     LREVYKSLAALKLKTEFVGYDRLEAEGKILKIFRGDAEVESGKEGEEVEVITDRSPFY
                     GESGGQQGDQGILSRNGARMEVSRSSRPLPDVVVHHGTIQSGVFRVGDTLKLRVNAES
                     RVQTALNHTATHLLQASLRQVLGDHVKQAGSLVAPDRLRFDYTHFSPLSDEELDRVEE
                     IVNSRIRENLEVCVSHISYKEAIQKGAMALFGEKYGEVVRLVQVGDVSAELCGGTHTA
                     RSGDIGLFKILSETGVASGVRRIEAATGEWAWKILKKEEAELRAISNLVKAKPGEAAE
                     KVQRILKQQKELERDYHALLAKIASGKTKDLISSAREVKGIRVLSTQVEAKDPKNLRE
                     MADRLKDQMKSGIVLLGAQGDGKVMLLCAVSADLAGKYPAQKLVKEIAQIVGGTGGGR
                     DDMAQAGGTKVEGLPEALEKIYDLI"
     gene            complement(3730..4221)
                     /locus_tag="VEL68_05995"
     CDS             complement(3730..4221)
                     /locus_tag="VEL68_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HXZ35552.1"
                     /translation="MPSKQLKPAESAEASAIRLLGRRDYSRSELRRKLIGKGFSAGEV
                     DGVLTQFDTRGLLNDRKLAQRLAGYYSREKLWGPQRLLQKLVQRGIPVEMAREVMHQE
                     EESGKTPERLRAILRHKLKGQDSLSLSVQDKQRLANYLRQRGYAWDDIWEALQEIGGL
                     LEE"
     gene            complement(4305..5324)
                     /gene="recA"
                     /locus_tag="VEL68_06000"
     CDS             complement(4305..5324)
                     /gene="recA"
                     /locus_tag="VEL68_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXZ35553.1"
                     /translation="MPAESQKEKAIDLAVSQIERQFGKGAIMRLGTDAVLPEIPVIST
                     GSLALDIALGVGGIPRGRVVEIYGPEASGKTTLALHIVSEAQKRGGIAGFIDAEHALD
                     VAYAQKLGVRTDDLLISQPDTGEQALEITDMLVRSGAIDVVVIDSVAALVPKAELEGE
                     MGDAHMGLQARLMSQALRKLTSTISKSNTCVIFINQIRMKIGIFMGNPETTTGGNALK
                     FYASVRLDIRRVGAIKDGQDVIGNRTRVKVVKNKMAPPFKEVEFDIMYGEGISREGDL
                     IDLAVEKEIIQKSGAWFSFDEERIGQGRENSKAFLKEHPEVATAIEEKIKDRFGLVKK
                     PKETQ"
     gene            complement(5338..5910)
                     /gene="thpR"
                     /locus_tag="VEL68_06005"
     CDS             complement(5338..5910)
                     /gene="thpR"
                     /locus_tag="VEL68_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXZ35554.1"
                     /translation="MDDIRSFIAIEVPQELKSRMEEVQRELRRTDADVKWVRPDAIHL
                     TLKFLGSIRREDVERISQAVGPVIGLWQPFEVCLQGMGCFPNLRNPRVVWVGVDRGKE
                     SLSSLKQQVEKRMTELSFPPEDRPFSPHLTLGRVRSLRGKADLSQAVESRKAVEVGIF
                     QAREVILFRSELKRSGAVYTKLKEFPMGKE"
     gene            complement(5963..7246)
                     /locus_tag="VEL68_06010"
     CDS             complement(5963..7246)
                     /locus_tag="VEL68_06010"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HXZ35555.1"
                     /translation="MRVELITIGNELTSGEVVDTNAAFMAAALSEVGLEVAFITTTGD
                     FPLSIQDALHKAQERAEVIIVSGGLGPTQDDITASSAAKALGLHLVLREEILQDLKKR
                     FAERRMQMPASNEKQAFFPQQAEIIPNPLGTACGFLVRRPGKIFFFLPGVPRELNFLM
                     KENVIPLLQKERKEKFRVRSRTLKVFGFTESAIADLLKEVSPEYFSASLAYLPRFPEN
                     HVKITLRGNSIEEVEGNLERLEKIIRDRLQGRVIAADEDTLEAMVGRLLREHQATLAV
                     AESCTGGLVAHRLTQVPGSSDYFERSVVVYSNKAKIQMLGVPESLISSLGAVSAEVAE
                     RMAEGVRQVSGTTLGLGITGIAGPTGGSEEKPVGTVFIALACANGTVSKRYQLPGDRN
                     QIQTLSAYLAIDWVRRYFLNLLSGFSSQPSARTNP"
     gene            complement(7268..7744)
                     /locus_tag="VEL68_06015"
     CDS             complement(7268..7744)
                     /locus_tag="VEL68_06015"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HXZ35556.1"
                     /translation="MSRLIIALASAGYAGFFPWVPGTLGTLVGVLIYFLFSFLSPSIY
                     LLATGAFFFLACWAAGKAEEILGKRDSPKIVIDEVTGYLIAMTWLPVSPACLASGFLF
                     FRVLDIIKPPPSGWVHAKMTGGMAVVLDDAVAGVYTNLLLRLILVYFPDFVLFGSD"
     gene            8106..9803
                     /gene="pilB"
                     /locus_tag="VEL68_06020"
     CDS             8106..9803
                     /gene="pilB"
                     /locus_tag="VEL68_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HXZ35557.1"
                     /translation="MSGRLGDLLVSNGLITPPQLEKALQEQKISGNKLGSALVKLGYI
                     SERNLVSFLSRHYGVPAIDLNEVQIDPEATKMIPADVIFKYQAIPIKRVGSTLRVAMC
                     DPSNILAIDDIKFLAGCHVEVFVSTESAIKGSIDRFYDASDTLNEIMGNIEEGEKMEL
                     LEDSEEINVNELQQAVEDAPVVKLVNLILNEAIKRSASDIHIEPYEKVFRVRLRVDGV
                     LYEFMKPPLKLKNAITSRIKIMSKLDIAERRLPQDGRIKVKYGKDKEMDFRVSVLPTL
                     FGEKVVMRLLDKSSLQLDMTKLGFEENPLKDFQSAIHKPFGMVLVTGPTGSGKTTTLY
                     SALTELNQISENISTAEDPVEYNLMGINQVQIHEEIGLSFANCLRSFLRQDPDIVMVG
                     EIRDFETAEIAIKAALTGHMVLSTLHTNDAPSTISRLLNMGIEPFLVASAVNLIVAQR
                     LARRICEGCKAVIQTSPQMLLNLGLSEEELQGFTCYKGAGCARCNNTGYKGRVALYEV
                     LPVREEIRELILQGASAGEIKKGAIQLGMKTLRQAALQKLKDKVTTVEEVLKTTVED"
     gene            9837..11051
                     /locus_tag="VEL68_06025"
     CDS             9837..11051
                     /locus_tag="VEL68_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXZ35558.1"
                     /translation="MDVFVWEGRSRKGTIEKGEMEAASESAVRMALRRQQIQATKIQT
                     KPKDILKGIKLPWKGRAREKEIVVFTRQFATMIDAGLPLVQCMEILSSQQTNPFFKET
                     LLKVKGDVEAGSTFADALRKHPSVFNDLYCNLVAAGEAGGILDTILNRLAAYIEKSMN
                     LKKKVKGAMVYPTAVMVVALVVVGALLLFVIPVFQKMFADMGGTLPAFTQLVINISHA
                     LRTFWWAFLALFIGLFLGFRTYYKTEKGREKVDNYLLQLPVFGTLIKKVAIAKFTRTL
                     STMLSSGVPILEGLDIVARTSGNKTIEKAILKTRVSIGEGKTVAEPLGTSGIFPPMVV
                     QMISVGESTGALDSMLAKIADFYDDEVDAAVAALTSLLEPILMVFLGTTIGALVIAMY
                     LPIFQMASVLGN"
     gene            11357..12388
                     /gene="tsaD"
                     /locus_tag="VEL68_06030"
     CDS             11357..12388
                     /gene="tsaD"
                     /locus_tag="VEL68_06030"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXZ35559.1"
                     /translation="MKILGIESSCDETAAAVVQEGQKVLSNIVASQVSVHRKYGGVVP
                     ELASRHHLEAILPVIHEALFQAEMELKDIDGIAVTQGPGLVGSLLVGIATAKSLSYVC
                     NLPFTGVNHILAHVAAIFLSIPHLKFPFLALVVSGGHTSLFRTESRTRMTLLGRTRDD
                     AAGEAFDKVAKLLELGYPGGEIIDRLSKEGNRNAILFPRTLLETESLDFSFSGIKTAV
                     LNRVKGMGQKPSPEQIRNIVASFQEAVVDTLVSKVLRAARREGIQEVVVAGGVACNSR
                     LREKFYQEGETWGISVFFPPPVLCTDNAAMVGAAGFYLLQEGTCADLTMNAYSRMGGE
                     SSSGWKTGS"
     gene            12370..12900
                     /locus_tag="VEL68_06035"
     CDS             12370..12900
                     /locus_tag="VEL68_06035"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HXZ35560.1"
                     /translation="MEDGELKRDGNGKNLGARWRRGLRLFYLRILRLRGKPEEVAGGI
                     AIGVAVGLTPTVPFHLVLAVLIAFLLGKSKLAAALGSQVGNPFFLPFIYFLDYKVGQA
                     ITGTSGPSLEFPDVSASHILNLGWNVYYPLLVGGVGVGLASVLPAYFLTRKLVLLSRE
                     RRRKRLEKIGFASKTT"
     gene            12875..13705
                     /gene="rsmA"
                     /locus_tag="VEL68_06040"
     CDS             12875..13705
                     /gene="rsmA"
                     /locus_tag="VEL68_06040"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXZ35561.1"
                     /translation="MASLQKQLKELQIRPKKSLGQHFLHDPNILRKIVEEASLEPEDV
                     VVEIGSGLGSLTEPLARRVKKVYALEIDSSLADALKDRFSGMETVEVIWADALQFDFG
                     APVRKWKRKIKVVANLPYEISSLMIFRLLKERECFSLMVLMLQLEVARRIAAKPGTKD
                     YGPLGVWSQLYSDVQIAFQVKPNSFTPPPQIQSAVVKFKVLPKPRVEVEEEKTLERVI
                     RSAFTYRRKTLSRALQMGGFPRPQVQEALRTSGIPTAIRGEALSLEKFCSLARLLGSK
                     "
     gene            13702..14850
                     /locus_tag="VEL68_06045"
     CDS             13702..14850
                     /locus_tag="VEL68_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35562.1"
                     /translation="MKKRFLLLCCIVALLAVLSGIYGERAFRSILFLSELFHFDKPGW
                     TEKDSSRPGIKEVSWEGPQGISKADLYLPSPEGKRGGILVNHGVIDTGKDDPRLKRFA
                     TGLCQAGFVVIVPDLKGMRSFRIAPADVDEIRAAFSHFLSRPEVRSDSCGLFGFSYGA
                     GPTIIAACQPEVREKVRFVVSFGGYYDLKNVLSFIATGYSEFQGKRYFRKPQEYGKWV
                     FLANNLDWVESGEDRTVLRKILRIKLRDENAPIDQFLSQLGGEGRNVLNLLSHADPSQ
                     TESFVRKLPPAIRSSMAHLSVSRVLKDLRAHLILAHGEDDDLIPFTETLRLAQAAPHS
                     EKVCMRILKTFSHIDPEQRPLSPGNLFSFYLPESWKLFMLTYRLLGYR"
     gene            15008..15745
                     /locus_tag="VEL68_06050"
     CDS             15008..15745
                     /locus_tag="VEL68_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ35563.1"
                     /translation="MSKLYLHLRKRFEQAVLKHGMLAEGDRVLVGVSGGADSLTLLKL
                     ITGPLLFVPKPEFVLPVYVDLGFEGTNGQPARVMKDYFEKEGGQYRIEKSNIGLLSHS
                     DYNRKASPCFLCSRLRRRKLFELARDYKCNKLALAHNKDDVIETLLLNVFFGREISTM
                     IPCQSYFQGEFYLIRPLAYTDEYLLKRFAREAGLPVMDNPCPTANNTKRKYIKDLIKK
                     LDQDHRGVKENIFKAMMHVKPDYLLTK"
     gene            15915..15988
                     /locus_tag="VEL68_06055"
     tRNA            15915..15988
                     /locus_tag="VEL68_06055"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15949..15951,aa:Met,seq:cat)
     gene            16234..18819
                     /gene="leuS"
                     /locus_tag="VEL68_06060"
     CDS             16234..18819
                     /gene="leuS"
                     /locus_tag="VEL68_06060"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXZ35564.1"
                     /translation="MTRRYDPQSTEEKWQKIWEERELFKSNGDPARQKYYVLEMFPYP
                     SGKIHMGHVRNYSIGDVVARYKRMRGWNVIHPMGWDAFGMPAENAAIQHGVHPAQWTY
                     ENIAVMKRQLRRMGFSYDWDRELATCDPSYYRWEQWLFLKMYEKGLAYQKTSFVNWCE
                     KCQTVLANEQVEAGRCWRCGEEVTQKEMPGWFFKITHYTEELLEYCEKLPGWPERVIT
                     MQRNWIGRSVGATIHFPFWDREGFIPVFTTRQDTVFGATFMTLAPEHPLTLELSKGTP
                     QEKEVREFVERIKKQDKIKRTSDDYEKEGMFIGAYCRNPLTGGKMPIFAGNFVLLEYG
                     TGAVMAVPTHDQRDFYFAKKFGLDLIVVVQPPGKPTLDAQTMAEAYTEDGVLVNSGRF
                     NGMTSEEAREAIAQYLEEKGSGKKAVQYRLRDWGISRQRYWGAPIPVIFCPQCGTVPV
                     PEKDLPVILPQDVKLTGLGDSPLAHAPSFVNVPCPRCGRPARRETDTMDTFVESSWYF
                     ERFCSPREDRGMFDREEVKYWMPVDQYIGGIEHAILHLLYSRFYTRVLRDFRLLDFPE
                     PFTNLLTQGMVVKETHHCSKDGFLYPEEVSENLTCQKCGSPVQVGRTEKMSKSKKNVV
                     DPDFLVQKYGADTVRLFCLFASPPEKDLEWSEQGVEGSFRFLHRVWRLVEDWADVVKT
                     AKARQKEELLSEELRALRRKIHVTIKRVTEDIENRFHFNTAISAVMELVNSLYQMEEK
                     RKTPGPQTAGVMREAVEAVVVLLSPFVPHICEDLWEYLGHEQSVAKEPWPQFDVEAIR
                     DEEVLMVIQVNGKLRSRVTVGVHASEAEIKEAVLANERVQEILQGQKVRKFVWVPKKL
                     VNLVV"
     gene            18912..21665
                     /locus_tag="VEL68_06065"
     CDS             18912..21665
                     /locus_tag="VEL68_06065"
                     /inference="COORDINATES: protein motif:HMM:NF017459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNA1162 family protein"
                     /protein_id="HXZ35565.1"
                     /translation="MKRRVGILWGITAIIIGIGIGGCIYTPQTKITPPITIPFKGEYR
                     FDKSLQDRPPQTVAVLPFLNRTEHKHAFDIVRKSFHGHFSTLNFTAVPLYKIDYALNQ
                     AGLDTPEKVAATPVSKLREIVKADALVQGEITHYDRIYIGVYSQVAVGAEVRMVETKT
                     DKELWWAKDVSRKHAGGISTTPVGIILTAVSTALNMREIELLRSSDDLFREMVRSIPQ
                     PTLAQALRPPAIAILAHDGMRRTDRYALKAGDVLKVALEGDPRKKASFRIGNFKTDIP
                     MKEEDPGMYSGTYKVLPGDFVEEALITGVLADDQRNSTEWVDALGPVTIDTIPPAVPG
                     GIRGIGRDKRVELDWAKSPDKDIAAYKVYRSPTPLTGFQEVGKTELTLFADANLTNQT
                     TYYYRVAAIDLAGNESKPSDSIRATPIPPGPTPVRGTIQGEAIWFAGASPYILEDRVS
                     VETRATLTIEPGTVIRSKGEGLVVSGKLLAQGDRSAMISFEPVSPGGEWKGILFQGSQ
                     SEESAVRYVKISGAQAGITCLSASPLILGNDLSKNRVGLRIQESFSKPRVQGNVIYAN
                     TAGVEILGGAAPILEENEIQGNQKDGVLVRESNPSLQKNRILNNGEAGVRLFSSSARL
                     TQNNIHDNGKFEVFNNRERDLPVEAPENWWGTKEGSSLAGKIYGRVEYQRVLDAPFPQ
                     GKPIELPLLKSPLNAPIQKDSFLILAYSPYVVEREVMIDGGATLYIQPGVTLKFNPGA
                     SMVVKNGGIDARGTSDGAITFTSSSSSPSPGAYPAAIRFAKETGVASFFRYCIIEFAE
                     TGMEVAYGSPEIDRCLIARSAQAGIKVTQEGAPKISYSTFLQNAGTGALVALGGARPK
                     VHRSNFRENDFAVQSHSSIFMDVRENWWGASPPSESIFLGQMNLKPWLEKPEPEAFGG
                     RKP"
     gene            21662..22729
                     /locus_tag="VEL68_06070"
     CDS             21662..22729
                     /locus_tag="VEL68_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35566.1"
                     /translation="MKGFIWGGLVFFFLALGPGQACAQPREVIEQVGNGVINWSQGII
                     LAKGSGSPPKDSRNIAQARLMTERAALTDARRNLLEVLKGVRVDSSTRVENFVTKDDQ
                     IRLQAEGFIQGSVEVRRFRKYLSDGAIEVTVAIDLSGDFISLMLSASEELRKKATPPP
                     APLVEAKPEKKTLPPPSPPAGPAVQLAPPQEKKPELPPSAPPMVKEVKPIPPPPVPAP
                     ERKPEPPPPPAKVGEAPLPDPATLPPFTGVVIDTRGLKMKPALAIRILNEGAKELYRG
                     EYVPQDKAARNGLALFARDLTAAQTNPRVGKNPLTIKGSKLDPAAPSDIILDQGEAQK
                     MAPFAQKGTFLEECRVMIVLD"
     gene            22739..23251
                     /locus_tag="VEL68_06075"
     CDS             22739..23251
                     /locus_tag="VEL68_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HXZ35567.1"
                     /translation="MRRLSLGIFLGFLCLAGCGYQLRGRETNLPPGIYSVAVPIFANR
                     TDQTGIETEVTRALVEKFISTKRLSVGPRNSADSLLTGTVKSFTTFPVAVVSSTQSAT
                     EYRATVILEYSFQGQKDGKVLFREEISEWRNYPVVSDLNVTEQNKLDAIRSISVLLAE
                     RIHEQILGGF"
     gene            23226..24338
                     /gene="holA"
                     /locus_tag="VEL68_06080"
     CDS             23226..24338
                     /gene="holA"
                     /locus_tag="VEL68_06080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXZ35568.1"
                     /translation="MNKFWAVSDATQNPCLLRPKDMKASEFFREIGRGNLLPLYYFWG
                     PEKWLIDDALKRIEDRALSPITRDFNREAFDAEEAETESILSSLQAFPVRSAWRLVII
                     RKADEIWKRTPSPFVDYFQNPNPQTCAVFIGARADLRAKFFQSLERKGAVVPFYPPFE
                     RDLVRWVHLQAEQLGHNVSDEAVTLLVERVGPSLREILVELQKLSLRKEKGRGITEED
                     VFALTADCRPESPFEFPQAVGLLKREEALRLLHKNLQQGEPPILLFSLLVRQLRLIQR
                     AQEMRGRGLTKKEVEGKLRIPPRSAEEFWAQVEIFPSPIREGLWPATWRTDQKMKLSR
                     GDKGLLLEEYVLRLLGDRAESQQPLRPPLRREKGEE"
     gene            complement(24341..24613)
                     /gene="rpsT"
                     /locus_tag="VEL68_06085"
     CDS             complement(24341..24613)
                     /gene="rpsT"
                     /locus_tag="VEL68_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXZ35569.1"
                     /translation="MATHDSALKRARQSERRRQRNSSVKSALKTITKKVLEAIDQKNP
                     ENARKALSSAIPAFQKAAGKKVIHKKTAARKISMLAKKVNSLPSGA"
     gene            24850..26421
                     /gene="murJ"
                     /locus_tag="VEL68_06090"
     CDS             24850..26421
                     /gene="murJ"
                     /locus_tag="VEL68_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXZ35570.1"
                     /translation="METQRRITEAASVVGGATLLSRIFGFLRDMIVAQLFGAGTATDA
                     FFVAFRIPNLLRRLVGEGSLTVSFVPVYSSYLTQKSKEESEELVSVCFTVLAFFLLLL
                     TAVGIFFSPWVVKIIAYGFSQDPEKFRLTVLLTRVMFPYIFFIGLVAWAMGVLNARKH
                     FAGPALAPVLLNLAIIACAILLSAHLGEPVMALAVGVLLGGVAQLLFQVPFLRRQEIR
                     LKLRFDLSHSGMRRVGALMAPSILGLAVTQLNVIVNTFLASYLPEGSVSYLYYADRLL
                     EFPMGVFAIAIATAVLPSLSEQAAKGKIEEVKETLSFALRLTFFVILPAMVGLVVLRT
                     PILNLLFQRGAFSAFSTEMTAQALLYYALGLVAFAGVRIIAPVFYSLQDTQTPVKVAF
                     VSLLSNAALGAILMFPLKHGGLALATSLAAGVNCALLAYLLRKKIGPMDIRRILHSFA
                     KNMTASILMGVTAYAIVYPGPWETSGITAEKMTLLGAAVGGGVLVYGTVSYFLGGDEM
                     RAAMKLAKKKAGRFL"
     gene            26508..26810
                     /locus_tag="VEL68_06095"
     CDS             26508..26810
                     /locus_tag="VEL68_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35571.1"
                     /translation="MQEIFLSYPQPFLLVGLKQNLKNCDNMSIIIKDLDIFEGFVISW
                     GMGWVNLTRWHRLMKRKIPFLQGDGKQKNQEIQNLVFHEEGGFGYIWHLLCYKKIP"
     gene            26947..27381
                     /locus_tag="VEL68_06100"
     CDS             26947..27381
                     /locus_tag="VEL68_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ35572.1"
                     /translation="MIAEFEIQGQDFKRAGEACFEIKNRLKRLGLPPGIIRRVATAAY
                     EAEVNVIVYAHKGSIRVNLDDQCVEVTVCDVGPGIPDIEQAMQVGYSTAPPYVKQLGY
                     GSGMGLPNIKKNSDWMEIQSELNRGTTLRFKVNLNESPQKDP"
     gene            27356..28693
                     /locus_tag="VEL68_06105"
     CDS             27356..28693
                     /locus_tag="VEL68_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HXZ35573.1"
                     /translation="MKVPKRILEDPGRAGGVFLSLKEDHCVGCTHCLRVCPTEAIRIR
                     GGKARMMPHRCIDCGECIRACPRKAWTVEAKPWERVLEKGKIVAVLDPAVLGQFGDRV
                     VPGMILRTFSEIGFSKVEDIGQGLAVYRRAVAGHLASGRAPSPAISSDCPAVVQLVQV
                     KFPSLLENLVPVVPPFEIMADLLRRNTEWRSTGGLGYVVPCLAKARAATEAGKPERSY
                     HHAIPFTEVYPILRAQLHGREEEPFPEAGGGPSVSALEWAYPGGQSRAMGLEASLIVD
                     GVSQVAEVLELAENGLLEEVPFIEAWSCRGGCLGGPLNVRNPFWARFQLFSWIRKDDA
                     RSPETLGRGEDVNAYIMDQPFPPRAGMRLDEDLQVAMEKLRRIDKVVKQFPGIDCGSC
                     GSPSCLALAEDVVQGYARETDCICILKKGKTVEAGPGKEASKGRPPKSRGKRR"
     gene            28715..29059
                     /locus_tag="VEL68_06110"
     CDS             28715..29059
                     /locus_tag="VEL68_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HXZ35574.1"
                     /translation="MKLKELAERLNLELTTPNAPMEAEVKTGYSSDLLSDVLANSIEG
                     DLWVTRQTHLNIVAIAVMRDLSGILLVNGSQPDPDTLEKAAEKMIPIFRTALPTFEIV
                     GRLYQLGISGQR"
     gene            29063..29653
                     /locus_tag="VEL68_06115"
     CDS             29063..29653
                     /locus_tag="VEL68_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ35575.1"
                     /translation="MLELSMHILDIAQNSLRAQAKGVEIRVQEDLGKDLLRIEVLDDG
                     RGMSPETVKKVTDPFYTTRPSRVAGLGVPFLKEAAEQCGGGLEVHSREGKGTRIVATF
                     QLDHFDRAPLGDMGETLAILITGNPGVEFLYEHRVNGRTYRLDTREMREILGPVGLGD
                     PSVFAFIRQDVQQGLKAIGAATFPQTMNLLGGRENP"
     gene            29650..31659
                     /locus_tag="VEL68_06120"
     CDS             29650..31659
                     /locus_tag="VEL68_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HXZ35576.1"
                     /translation="MTVISTIKEKCRRCYTCVRKCPAKAIKVEDGQAKVVAERCIACG
                     SCIKVCRQEAKAIRDSTGTVKDLFWNSPEIIACLAPSFPAAFPQASPGQIITAVRNLG
                     FTGVMEVAFGADLVAKAHARWTKRFADKPYISSPCPALILYIKKYFPPLVDHLVPIVS
                     PMVAMGRVIKRNIRPEAKVVFIGPCIAKKEEIEDPEVAGDVDAVLTFVELDRMFQEAG
                     LIPEKLAESPIDGPLPRMGRIFPVPGGLLRSASMKEDVCQNRILVTEGKEACTQILNE
                     ILEGSVEAKFLDLLFCEGCINGPGFPNELSVFSRKELVANYVQSRLRAEREDRHRADM
                     RKHSRISLGRSFSAEDRRLTTPSPDIIREILRRTNKVHPEDELNCGACGYASCQEKAS
                     AVYWGLAENEMCLPYLIDQLEENLSRLAQSHKDLRETQQQLIQSEKMASVGQLAAGVA
                     HEINNPLGTILLYSHMILEKLEQKDSRREELDTIAKEATRCRDIVRGLLDFARQRKLQ
                     VENVDVNKILEEGLSLVAAQPAFQKVEIAKTLDPSLPATEGDPVQLKEVFLNILSNAG
                     EAMPEGGRVTVVSRFREAGSHPIEIMIRDTGQGIPQENLNKLFMPFFTTKRIGQGTGL
                     GLAIAYGIVKMHRGAIEVQSKVGEGTTFWVKLPAESSLRSQESVG"
     gene            31705..32124
                     /locus_tag="VEL68_06125"
     CDS             31705..32124
                     /locus_tag="VEL68_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ35577.1"
                     /translation="MKGSPKVLIVDDDPDFIEIGKLSLEGKGYQVLSASTGREGWEMV
                     EKEIPDLVILDLMMEDLDAGMTLSSRIKSHPRYASIPILMLTSISRETGMDFTPRTEE
                     DLRKLKVDDFHSKPIKVKVLQEKVEKLLSRGSPSGSR"
     gene            32138..33607
                     /locus_tag="VEL68_06130"
     CDS             32138..33607
                     /locus_tag="VEL68_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ35578.1"
                     /translation="MKGARILIVDDEPPIRAACAKILSEQGALVETAEDGLAGLEKAR
                     TQEFDLALIDLKMPQMDGMQLLEHLNQLDPDLVKIVITGFATLDTAIEAVQKGAYDYL
                     PKPFTPGEMRTRVRRGVEKRTLLREAKRLREERERNLLELTNEKSRTQTIIQCMGDGL
                     LVTNRDQQVVLCNPAARRLLKIKRPLQGGEPLSNVAGSAEFLDLVEGTMGLKEGQEMS
                     SREVAPASPSGPVLMANCAPVKDEKGQIIGVVTVLRDITELKALDKAKSTFVSLVAHE
                     LQAPLAAIEGYLDIILDGGGGKDPEKTRKILDRCRERTSGLLALIQDLLAISRMQSGR
                     IVREKEKLRLTDVLKEVVELMKGEALERGVKIAAELPEELPPISANREDLTRVFTNLM
                     DNAIKYNPAGGRVFLRAKALDAFLQVEVQDTGIGIPAEEKEKIFDEFYRVKNKDTKGI
                     GGTGLGLSIAKKILEAHNGHLEVESQLHEGSTFRVLLPL"
     gene            33853..34101
                     /locus_tag="VEL68_06135"
     CDS             33853..34101
                     /locus_tag="VEL68_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HXZ35579.1"
                     /translation="MERRLGVVGIVIEDRKKSAPQVNQVLSEHGEIIAGRMGLPYGRR
                     NVSVIALIVDGTTDELGSLAGKLGMLNGVQVCSSLFRR"
     gene            34140..35537
                     /gene="hydG"
                     /locus_tag="VEL68_06140"
     CDS             34140..35537
                     /gene="hydG"
                     /locus_tag="VEL68_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HXZ35580.1"
                     /translation="MAEKAKIIDEERIQRNLEATKGKSREEVLEILAKAEEAKGLEQA
                     EAAALLQMEDPALLQEIFETARRVKLKIYGKRLVLFAPLYLSNHCVNNCSYCGFRRDN
                     RLIPRKRLTRAEIRAQVSELEKLGHKRLLLECGESSLSDMDYVEEAIATIYATKTEKG
                     EVRRVNANIAATTVENYRRLKAAKIGTYQLFQETYHRATYLEIHDGPKRDYERQILAL
                     DRAQEAGIDDVGLGVLFGLHDYKFEILAILSHARHLEERFGVGPHTISVPRFRAARGV
                     SFLPPHPVTDQDFKRLVAVLRLCLPYTGIIISTRESMAMRSEAFSLGISQTSAGSRTS
                     PGGYGAKGEGNEEAGPGAQFETADHRLPDEVIRSICEEGYLPSFCTACYRVGRTGKDF
                     MAVAKPGDIQHLCGPNAILTFKEYLLDYAPEETREVGEIALKQHLEDISNQSLKEKTR
                     ERLEEMENGKRDLYF"
     gene            35615..36667
                     /gene="hydE"
                     /locus_tag="VEL68_06145"
     CDS             35615..36667
                     /gene="hydE"
                     /locus_tag="VEL68_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HXZ35581.1"
                     /translation="MIRPGFLETLEAGEKGKVLSSGEMELLLSADPEEREALFQAADR
                     VRRRFVGEEIHLRGIVEFSNICVQNCLYCGLRKDNRDLRRYRMTLGEVQESAKAIQGM
                     GIKTLVLQSGEDPWYTRDRLSELIRRIKEETGLTLTLSVGERPREDYQAWKDAGADRY
                     LLKQETSAEQLFAYLRPGRTLTERLESLRFLRELGYEVGSGNMVGLPGQSHNDLVRDI
                     QLFREFDFDMIGIGPFIPHPQTPLGNSATPDLNLTLKVLALTRIVTRDPHLPATTASA
                     VLEPEGRKRALLAGANVIMPNLTPWQYRGYYQVYPGKEKPDGGLDSTLRTISSLGRTI
                     SQGPGYRRSLPGKRSS"
     gene            36914..37156
                     /locus_tag="VEL68_06150"
     CDS             36914..37156
                     /locus_tag="VEL68_06150"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HXZ35582.1"
                     /translation="MDKFDILEEKINQLVDAYSALRREKDSLGARLIEKESEIHKLNE
                     RINQFSRERDMAREKVEGLLHRVERLIASPAGMGKG"
     gene            37153..37443
                     /locus_tag="VEL68_06155"
     CDS             37153..37443
                     /locus_tag="VEL68_06155"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXZ35583.1"
                     /translation="MKNLVRVEILGREYTIKSDEGEERVKKIAQYVNAKLKKISESAQ
                     TLSTHNAAILAALDIANDYFEVLEGRTSLSQKVEKMEKQTGRLIEIIDSKIH"
     gene            37459..37649
                     /gene="ssrS"
                     /locus_tag="VEL68_06160"
     ncRNA           37459..37649
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VEL68_06160"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            38024..39571
                     /gene="rny"
                     /locus_tag="VEL68_06165"
     CDS             38024..39571
                     /gene="rny"
                     /locus_tag="VEL68_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXZ35584.1"
                     /translation="MIGLGLVLLAAGLLIGFFLHQKLAAARIRLAEQNTREVVEEAKK
                     KAEAMRREAEIQAKDFLFQAKTEFEAEVKDRRQEIGNLEKRFLQKEENLEKRIDLFEQ
                     KEANLSKREKSILQQEKSVGDMEKKYQSLLGEQRQLLERIAGISSDEAKDRLMKMVES
                     EMRHESAKLIKRIEEETQGQAEKKAREIIGNAIQRYAADYVAEETVSVVNLPNEEMKG
                     RIIGREGRNIRALEAACGVDLIVDDTPEAVIISGFNPVRREIARITLERLITDGRIHP
                     GRIEEIVAKAGEEVEKAIREGGERATFDVGVHGINPELIKMIGKLKYRTSFAQNVYQH
                     SLEVAFLAGAMASELGVNVKQAKRAGLLHDIGKAVDHEVEGSHALIGADLARKFGEGQ
                     KIVHAIAAHHDDEKPAGLLDILIQAADALSAARPGARWEMLETYVKRLEDLERIALSF
                     PGVGKTYAIQAGREIRVIVENDEISDADSVMLSRDIARKIENELSYPGQIKVTVIRET
                     RAIEYAK"
     gene            39861..>40254
                     /locus_tag="VEL68_06170"
     rRNA            39861..>40254
                     /locus_tag="VEL68_06170"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         9211 a        10866 c        11454 g         8723 t
ORIGIN      
        1 cctacatcac cggcaaaatc atctacatgg acgggggaat gttgtaggcc tggccgaggg
       61 accggtttat ccttaaaagc gagtctgtcc ctgtaaaggg ttccagccct cctttaccaa
      121 tgacccccgg gtggaacgga agataaacct gacggggatg aagttctaga aaacgcgtaa
      181 ttcggatttc gggatgcgtt cggattgaca ggaaagggtg gggcatctat attggagata
      241 ggtccttcta aacaggcgca ggagatgatt gcaagtatga atggggctaa aagaaatttt
      301 agcaataaat tattgttgcc gattctctcc tccgtctttc tgctggtttg gagtaatggc
      361 tgtgcccttt tcgggaagaa agagccggca acgactcccc aagcggcata tgatgaggga
      421 gtgctccttt tggaaaagaa gaaatatgag cgctcggccg aggctttccg gaaattcaaa
      481 gaagattatc ccttaagcac ttacaccccc ctcgccgaac tgcgcctggg agactcgctc
      541 tacttcgaca agaattacgc cgaggccatc gtccagtatg aagaattcaa aaagctccac
      601 cccgtccatg cggagatccc tcacgccatc taccagatcg gcatgtgtta tttcaagcaa
      661 atgctctcca tagaccggga tcaaacagtg acggaaaaag ccctggaaca gttccgttac
      721 gtggtggaga attttcccca gagcaaatac cttcccgacg ctaaaaccaa aatgcagctt
      781 tgccagcgcc agcttgccga ccaggaattt gacatcggct atttctacta tcggatggga
      841 tattacaaag ccgccctggg gcgcttcgag gagattctga aaaagtttcc ggatgccggg
      901 ctggagcgga aaatcacccc cctcatgaaa acctgccgtg aaaagatcgc caaagaagag
      961 aagaaattaa aggagaaaga agggaaggaa gcaaagaaaa aacaatcgta aatccagcat
     1021 aacccggaga acatagagta aaaagcggcc agaaattctc cctatgccct tcgctctatg
     1081 ttttctagat gaggtcgtag attttttcca gcgcctccgg caatccttcg accttggttc
     1141 cgccagcctg ggccatgtcg tcccgtcctc caccggtccc cccgacaatt tgggcgattt
     1201 ctttgaccag tttctgggct gggtactttc cggctaagtc cgctgacacc gcgcagagga
     1261 gcattacctt tccatccccc tgggcaccca ggagcacaat accagatttc atctggtctt
     1321 tcaggcggtc ggccatttcc cggagattct ttgggtcctt ggcttccacc tgcgtggaaa
     1381 ggacccgaat ccccttgacc tcgcgggcgg atgagattag gtctttggtt ttccccgatg
     1441 cgattttagc caaaagggca tggtaatccc tttccagctc tttctgttgt ttcaggattc
     1501 gttgcacctt ctccgccgcc tctcccggct tggcttttac cagattggat atcgcccgaa
     1561 gctcagcctc ttctttcttg aggattttcc aggcccattc tccggtggcg gcctcaatcc
     1621 gccgcactcc ggaggccact ccggtttcag aaaggatttt gaaaagcccg atgtccccgc
     1681 tgcgcgccgt gtgggttccc ccgcacagtt cggcgctcac gtccccaacc tgaaccaatc
     1741 ggaccacctc cccatacttt tccccgaaga gcgccatagc ccctttctgg atggcctctt
     1801 tgtaggaaat atgggaaaca caaacttcca ggttctcccg aatccgggaa ttgacgattt
     1861 cctcaacccg atccagctct tcatcggaga gaggggaaaa gtgggtgtag tcaaaacgga
     1921 gtcggtcggg agccaccaag gaccccgcct gcttgacatg atctcccaac acctgtcgca
     1981 aggaagcctg caggagatgg gttgccgtgt ggttaagggc ggtctggacg cggctttcag
     2041 cattcacccg cagcttgagg gtgtctccca cccggaaaac tccggattga atggtcccgt
     2101 gatgaacgac cacatcgggc aggggtctgc ttgaacggga aacctccatc cgggccccgt
     2161 tgcgggacag aatcccctgg tctccctgct ggcctccaga ttctccgtag aaggggctac
     2221 ggtcggtgat cacttccacc tcttctcctt ccttcccgct ctccacttcc gcatcgcccc
     2281 tgaaaatttt caggatcttc ccttccgcct ccaagcggtc atagcccacg aactcggttt
     2341 tcaacttcag ggcggccagg gacttgtaga cttcccgcag ggcctcttcg cccgaaccct
     2401 tccaggactc acgggcccgt tcgcgctggg tctccatggc atcttcaaat ccctttttgt
     2461 ccaggctgaa tccttcgtcc cgggcgatct cctcggtcaa gtctaagggg aacccgtacg
     2521 tgtcgtagag cttgaagacc aattccccgg ggatgacctt cgactctcgg tccttcagat
     2581 tttggatttc atcggcccag aggcgaagac cggtattcag ggtctccgag aagcgatcct
     2641 cttcgttgga gatcatgttc ttgaccattt cgcgatggct ggcgagttcg ggataggcgt
     2701 cctccatcag ggcaatcacg tcatccgtga gacggaaaag gaaaggctcg ttgatcccca
     2761 gtttcctgcc gtgccgtagc gcccgtcgca tgatcctgcg aaggacgtat ccccggcctt
     2821 cattggaagg gaggactccg tcgctgatca agaaagttgc cgcccgggca tggtcgccga
     2881 tgacccggaa ggaaactccc tcgggggacg aggggtcata ttttttcccg cttaatcccc
     2941 caatggcctt caagatccgg ccaaagaggt ccgtgtcgta gttgcttctt accccttgaa
     3001 cgacggcggt gatccgctcc agtcccatgc cggtgtcgat acttggcttg ggaaggggct
     3061 tgatggtccc gtcggcaaag cgttcgaact gcatgaagac caggttccac aattccagat
     3121 agcggtcgca ttcgcacccc aaggcgcact ccctgcgacc gcaccccact ccaggacctt
     3181 ggtcgatgac gatctcggag caagggccgc aggggcccgt atcccccatt tgccagaagt
     3241 tgtccttgct ggggaaggtc cggatgcgct cctccgggag gtattttttc cagatctgcc
     3301 tggcttcttg gtcgggtccg atccccaggc tcttctcccc ttcgtgaatg gtggcccaga
     3361 gcttgtcggg gggcaggccg aggaggtcca cgaggaactc ccaggccatg gcgatggctc
     3421 cttccttgaa gtaatcgccg aaagagaagt ttcccagcat ctcgaagaag gtgtgatgcc
     3481 gggcggtaaa tcccacattc tccaggtcgt tatgctttcc tccggcgcgt acacattttt
     3541 gggaagtcgt ggcccggggg aagggggcct tttcctctcc ggtaaaaaaa tttttaaact
     3601 ggaccatacc cgcgttggtg aagagaaggg tggggtcttt ctggggaacc aacggggaac
     3661 tgctcacgat ggtgtgccct tggctcttga agtaattcag aaatttctgg cgaatggcgc
     3721 tgcccttcat cattcttcta gcaaacctcc gatttcctgc aaggcctccc agatatcgtc
     3781 ccaggcatac cccctctgcc ggaggtagtt ggccagcctt tgcttgtcct ggaccgacaa
     3841 agaaagggag tcttgtcctt tcaatttatg ccgcaaaatg gcccgcagcc gctccggggt
     3901 ctttccgctt tcctcttcct ggtgcatgac ctcccgggcc atctcgaccg gaatccctct
     3961 ctggaccagt ttttgcagca gcctctgagg accccaaagc ttttcccgag agtagtaacc
     4021 ggccagccgc tgggccaatt tccgatcatt taaaaggccg cgagtgtcga actgggtgag
     4081 gaccccgtcc acctctcccg ctgaaaatcc cttcccgata agctttcgtc gcagctccga
     4141 acggctgtaa tctctcctgc ccagaaggcg gatggcggag gcctcggcgc tctccgcggg
     4201 cttcagttgc ttgcttggca tggaagggga gggggctttc taaatgcgaa atgccgagga
     4261 taaaaaatct tatcggagaa ttccgccttc ggcattccgc aactctattg ggtttccttc
     4321 ggtttcttca ccaggccgaa gcgatccttg attttttctt caatggccgt agccacctcc
     4381 ggatgctctt ttaaaaaggc cttggagttc tcccggcctt ggccgatccg ctcctcatcg
     4441 aacgaaaacc aggcgccgct cttctgaatg atctcctttt ccacggctag gtcgataagg
     4501 tctccttccc gggagatccc ttccccgtac atgatatcga attccacttc tttgaagggg
     4561 ggggccattt tgttcttgac cactttgact cgggtccggt tgccgattac gtcttgcccg
     4621 tccttgatcg cccccaccct ccgaatatcc aaacggacgg aagcgtaaaa tttcaaggca
     4681 tttccccccg ttgtagtttc tgggtttccc atgaaaatgc cgatcttcat gcggatctgg
     4741 ttaatgaaga tgacgcaggt attggatttg ctgatcgtgg aggtgagctt cctcagcgcc
     4801 tgggacatga gacgggcctg gagccccatg tgggcgtccc ccatctctcc ctccaactcc
     4861 gctttaggaa ctaaagcagc caccgaatcg atgaccacca cgtcgatggc tccgctccgc
     4921 accagcatat cggtaatctc cagcgcctgt tctccggtat caggctggga gatcagaagg
     4981 tcatcggtcc tcacccctag cttctgagcg taggccacat ccagagcatg ctcggcgtcg
     5041 atgaaaccgg caattcctcc tcttttctgg gcttcggaaa caatgtgaag ggccagcgtg
     5101 gtcttcccgg aagcttccgg accgtagatc tctaccactc gcccgcgggg aattcccccc
     5161 accccgaggg caatatccag agccagagag cctgtggaaa tgaccggtat ctctgggagt
     5221 acggcatcgg tccccaggcg catgatggct cccttgccga attggcgttc gatctggctt
     5281 acggccaggt caatggcttt ttccttttgg ctctctgcag gcatggaacc tcctttttta
     5341 ctcttttccc atagggaatt cttttaattt cgtgtaaaca gcccccgagc gcttgagttc
     5401 actccggaaa aggataacct cccgggcctg aaaaatccct acttccactg ctttgcggct
     5461 ttctacggcc tgggacaaat ctgctttccc ccggagggat cggactcttc cgagggtcag
     5521 atggggagaa aaggggcgat cttccggggg gaaggatagc tcggtcatcc ttttttcgac
     5581 ctgctgcttc aaagatgaga ggctttcttt ccctcgatcg actcccaccc aaaccacccg
     5641 aggatttcga aggttgggaa aacatcccat cccctggagg caaacttcaa agggctgcca
     5701 aaggccgatg accggcccaa ccgcctggga tattctctcc acatcctctc gccgtatgga
     5761 acccaggaat tttagagtca aatggattgc atccggtcgc acccatttga catcggcatc
     5821 cgtcctccgg agttcccgct ggacctcctc catcctggat ttcagctcct gaggaacttc
     5881 aatggcgatg aaggatcgaa tatcgtccat aaagacaacg atcagctgtc agcattccgc
     5941 tttcagcaga gacgaacctt aattacgggt ttgtcctggc tgaaggttga gagctgaaac
     6001 ctgacagcag attcaagaaa taccttcgca cccaatcgat ggcaaggtaa gccgataggg
     6061 tttgaatctg gtttcgatcc cctggcagct gataacgttt ggatacggtt ccatttgcgc
     6121 aggccaaggc gataaagacc gttcccacgg gtttttcttc cgatcctccg gtgggcccgg
     6181 cgatcccggt gatgcccaat cccagagtcg ttccgctgac ctgccggacc ccttcggcca
     6241 tcctttccgc cacttctgcg ctcaccgcac ccagggacga aatcaacgat tcggggactc
     6301 ccagcatctg aatcttggct ttattgctgt atacgactac cgacctctca aaataatccg
     6361 agctgccggg aacttgggtc aggcgatgag ccacaaggcc cccggtgcag gattcggcaa
     6421 cagccagcgt cgcctgatgt tcccggagga gacggcccac catagcctcc agggtatcct
     6481 cgtcagccgc gatcacccgg ccctgcaacc ggtcccgaat gatcttctcc agcctttcaa
     6541 ggtttccttc aacctcctcg atgctgtttc cccggagagt gatcttcaca tggttctcgg
     6601 gaaaccgtgg gaggtaagcc aggctggcag aaaaatattc ggggcttacc tctttcagca
     6661 aatcggcaat ggcggactcg gtgaatccaa ataccttcag ggttcgggat cggaccctga
     6721 atttttcttt tctttccttt tgcagcaggg gaatcacgtt ttctttcatc aaaaaattca
     6781 actcccgagg aactccggga aggaagaaaa agattttgcc cgggcgccgg actaaaaacc
     6841 cgcaggccgt gccgagcggg ttgggaataa tctccgcttg ctgggggaag aaggcctgct
     6901 tttcattgga agcgggcatc tgcatccttc gttcagcgaa gcgtttcttc aggtcctgaa
     6961 ggatttcttc gcggagaacc aggtggagtc ctaaggcttt ggccgctgaa cttgcagtga
     7021 tatcatcctg ggtcggtccg agacccccgc tcacaataat aacctcggcc cgttcctggg
     7081 ccttgtgtaa agcgtcttgg atggataaag ggaagtctcc tgtggtggtg ataaaagcga
     7141 cctccaggcc gacttcagac agcgcggcgg ccataaaggc ggcgttggta tccaccactt
     7201 cgccggaagt gagttcgttg ccgatggtaa tgagttcgac ccgcaaaaaa gccccccgga
     7261 ccgataatca atcgctccca aaaaggacaa aatcgggaaa atagactaaa ataagccgga
     7321 gcagtagatt ggtatacact cctgcaacgg catcatccag gacaacggcc atgcctccgg
     7381 tcatcttggc gtgaacccat cccgaaggag gcggctttat tatatccaaa acccggaaaa
     7441 aaagaaatcc gctagccagg caagcaggac tgacggggag ccaagtcatg gcaataaggt
     7501 atccggtcac ctcatcgatc acgatcttcg gactgtccct cttacctaga atctcctcag
     7561 ccttcccggc agcccagcag gccagaaaaa aaaaggctcc cgtcgcgagc aaatagattg
     7621 agggagaaag gaacgaaaac agaaaataga tcaacacccc caccagggtg ccgagggtcc
     7681 ccgggaccca agggaaaaac ccggcatacc ctgcggaagc cagagcgatg atgagacggc
     7741 tcaatttcct tcaccttgaa ttgggcccgt cggcccactc ttccatactc ctcattcggg
     7801 cggggtttga ttttcacacc gggaacgggg gtttaaaaat ttctcttccc gattggccca
     7861 aatcgttcgc ctgccttggt tttttccggg ctcttcggtg aagggaaaag taccctcttt
     7921 ctgaagagtt gtcaatagga agggactctg cccgtccttc ccatgggctt ttcaagactt
     7981 gagaaagact taagattttt cccctctttg acgataaagc tttagaatca attggaaatc
     8041 ccttgacaag acccggatag ccttctatat ttttaaaaaa cattataatt gtggagatat
     8101 agcatatgag tggccggttg ggtgaccttc tggtcagcaa tggcttaatc acccccccac
     8161 agctggaaaa agccctccag gaacagaaaa tctctggaaa caaactgggt tccgctctgg
     8221 tgaaactggg ttacattagc gaaaggaacc tggtttcctt cctcagcagg cattatggcg
     8281 tgccggccat cgacctgaat gaagtccaaa tcgatcctga agccaccaaa atgatcccgg
     8341 ccgacgtcat cttcaaatat caggcgatcc ccatcaagcg agtcggttca accctgcggg
     8401 tggccatgtg cgatccctcc aatatcctgg ctatcgatga cattaaattc ctggccggtt
     8461 gccacgtgga agtttttgtc tctaccgaaa gcgccatcaa gggctccatt gaccgtttct
     8521 acgatgcttc cgatacgctc aacgaaatca tgggaaatat cgaagaaggc gagaaaatgg
     8581 agcttctgga agattcggaa gagatcaatg tgaacgagct gcagcaggcg gtcgaagatg
     8641 cgccggtggt gaagctggtc aacctgattt tgaacgaagc gatcaagagg tccgccagcg
     8701 atatccatat cgagccttac gagaaagttt tccgggttcg gctgcgggta gacggggttc
     8761 tttatgagtt tatgaaaccc ccgctgaaac tgaaaaatgc catcacctct cggatcaaga
     8821 tcatgtccaa gctggacatc gccgaacgcc ggcttcccca agatggtcga attaaggtaa
     8881 aatacgggaa agacaaggaa atggattttc gcgtctccgt cttgcctacc ctctttgggg
     8941 aaaaggtggt catgcggttg ctggacaaat ccagcctcca gctggacatg accaagctgg
     9001 gtttcgagga gaatccctta aaagatttcc agtccgccat ccataagccc tttggcatgg
     9061 tcctggttac cgggcccacg ggaagcggaa agactaccac cttgtactcc gccctgaccg
     9121 agctgaacca aatttcggaa aacatctcca cggccgagga cccggtggaa tacaacctta
     9181 tgggcatcaa tcaggtgcaa atccatgagg aaatcggcct tagcttcgcc aattgcctcc
     9241 gttccttcct gcgccaagac ccggatatcg tcatggtggg ggaaattcgg gatttcgaga
     9301 ccgcagagat cgccattaaa gcagccctca ccggacatat ggttttgagc accctccaca
     9361 ccaatgacgc ccccagtact atcagccgcc ttttgaacat gggcatcgaa ccctttttag
     9421 tcgcttccgc ggtcaatctg atcgttgccc agcgtctggc gcggaggatc tgcgaaggct
     9481 gcaaggccgt aatacaaact tctccccaga tgcttttgaa cctggggctt tccgaggaag
     9541 agctccaagg tttcacctgc tacaaaggag ctggatgcgc ccgctgcaat aacacgggtt
     9601 ataagggccg cgtggccctg tatgaagtcc tcccggtccg ggaggagatc cgcgaactta
     9661 tcctccaggg agcctcggcg ggggagatca aaaagggggc gattcagctg gggatgaaga
     9721 ccttgcggca ggcggcgcta cagaaattga aggataaagt aacaaccgtg gaggaagtat
     9781 tgaaaaccac agtggaagac tagccgagaa ccaagcccga aacggggagg atggtaatgg
     9841 acgtatttgt ttgggaaggg cgatcccgga aagggacgat cgagaagggc gaaatggaag
     9901 cagcgagtga gtcggctgtt cggatggctc tccgccgtca gcaaatccag gccaccaaga
     9961 tccaaaccaa acccaaggac atcctgaaag ggatcaagct gccttggaag ggccgggccc
    10021 gggaaaagga aatcgtcgtc tttacccgcc agttcgccac catgatcgat gccggtttgc
    10081 ccctcgtcca atgcatggag atcctttcca gccagcagac taaccctttc ttcaaggaga
    10141 cccttttaaa agtgaaaggg gatgtggaag cgggctccac ctttgccgat gccctgcgca
    10201 agcacccctc ggttttcaac gatctctact gcaacctcgt ggctgctgga gaagcggggg
    10261 gaattctgga cactatcctg aaccggctgg ccgcctatat tgaaaaatcg atgaatctga
    10321 aaaaaaaggt caaaggagcc atggtgtatc ccaccgccgt tatggtggtg gctctggtgg
    10381 tcgttggagc cctcctctta ttcgtgattc ccgtcttcca gaagatgttt gccgacatgg
    10441 gaggaaccct ccccgcattc acccaactgg ttatcaacat cagccacgcc ctacgtactt
    10501 tctggtgggc cttcctggcc cttttcatcg gcctttttct ggggttccga acctattata
    10561 agaccgaaaa ggggcgggag aaagttgata attatctgct ccagctcccg gttttcggga
    10621 ccttgatcaa aaaggtggcc attgccaagt tcacccgcac cttgagcacc atgctctcca
    10681 gcggagtacc cattctggag ggactggaca tcgtagcccg cacttcagga aacaaaacca
    10741 tagaaaaggc catcctaaaa acccgcgtca gtatcggaga aggaaagacc gttgccgagc
    10801 ccctgggaac cagcggaatc ttcccgccca tggtggtcca gatgatctcg gtcggcgaat
    10861 ccacgggcgc cctggattcc atgctggcga agattgccga cttctacgat gatgaggtag
    10921 acgcggcagt ggctgccctc acctccctgc tggagccgat cctcatggtc ttcctgggca
    10981 ccaccatcgg cgctctggtt atcgccatgt accttcccat tttccagatg gcttccgtgc
    11041 tggggaacta gctgggtcct cccccgccgc tcggaatcag aactccgggt tccaggcgct
    11101 tggccgaggg gagaattttc aagcgggtct tgcctctcga gttaagctcc ggatatgcag
    11161 ggccggaggg cttccctcac ggtccgcatt atgatattgt tagacaaacc agtcttcccc
    11221 ccatatcggc gcatccgaaa aagggagccc actttccggg tattggggtc gggtctcggg
    11281 gcctgagcct cccagaaggt ccgggagctt tgggccaggc ccaaaaaaca ttgcccctcg
    11341 ataagttgat ccacagatga agatattggg gattgaatct tcctgtgacg agactgccgc
    11401 tgcagttgtc caggagggcc agaaggtctt atccaacatt gttgcctctc aggtttccgt
    11461 acaccggaaa tacggggggg tcgtgccgga gctagcctct cgccaccacc tcgaggcaat
    11521 tctgccagtc atccatgaag ccctttttca ggcagaaatg gagttgaaag atatcgacgg
    11581 gatcgccgtc acccaggggc cggggctggt aggctccctt ttggtgggaa ttgccacagc
    11641 gaaatccctg tcttatgtct gcaaccttcc cttcaccggg gtgaaccaca ttctcgccca
    11701 cgtggcggcg atttttctct ctattcctca tttgaaattt ccttttctcg ccctggtggt
    11761 ttcaggggga catacgagcc tttttcgaac ggagagcagg actcggatga ccctgctcgg
    11821 ccgaacgcgc gacgatgctg cgggcgaggc tttcgataaa gtggccaaac ttctggaatt
    11881 gggttacccc gggggggaaa tcatcgaccg cctaagcaaa gaaggaaata ggaatgcaat
    11941 tcttttcccc cgaactcttc tggaaacgga gtccctggat ttcagcttca gcgggataaa
    12001 gacagccgtt ctcaacaggg tgaagggaat ggggcaaaaa ccttccccag agcaaatccg
    12061 gaatattgtt gcctcgttcc aggaggcggt cgtggatacg ttggtctcca aagtcctccg
    12121 ggcggctcgt cgggagggaa tccaggaagt ggttgttgcc ggcggggtgg cctgcaattc
    12181 ccgacttcgg gaaaaatttt atcaagaagg agagacctgg gggatttcgg ttttctttcc
    12241 tccccccgtc ctctgtacgg acaatgcggc catggtgggt gccgctggtt tctatctcct
    12301 ccaggaaggc acttgtgctg acttgactat gaatgcctac tcccgcatgg gaggggaatc
    12361 gtcatcggga tggaagacgg ggagctgaaa agggacggga acgggaaaaa cctgggggcg
    12421 cgatggaggc gtggcctgcg tctcttttac ctccgcatcc tgcgccttcg ggggaagcct
    12481 gaggaagtgg ccggcggaat agccatcggg gttgccgtcg gcctcactcc gacagtgccg
    12541 ttccatctgg ttctggctgt cttgatcgct ttccttctgg gcaaaagcaa gctagccgct
    12601 gctctgggga gccaggtggg gaaccccttt ttccttccct tcatctattt tctggattac
    12661 aaagtggggc aagcgatcac tggcaccagc ggtccttctc tcgaatttcc cgacgtctcc
    12721 gcctcccaca tcctaaacct gggctggaat gtttattatc ccctgctagt gggaggggtg
    12781 ggggtaggcc tggcttccgt cctgcccgcc tatttcctta ctagaaagct ggtcctcctc
    12841 tcccgggaaa gacgaagaaa gcggttggag aaaattggct tcgcttcaaa aacaacttaa
    12901 agaactgcaa atccgcccga aaaagagtct gggccagcat ttcctccacg atcccaacat
    12961 tctccggaag atcgtcgaag aagcttctct cgagccagag gatgtggtgg tggagatcgg
    13021 ctcgggcctg gggagcctga ccgagccttt ggcccggcga gttaaaaagg tttatgccct
    13081 ggagattgat tcgtcgctcg cggatgcttt gaaggaccga ttctccggga tggagactgt
    13141 ggaagtgatc tgggcggatg cgttgcagtt tgattttgga gccccggtac gcaagtggaa
    13201 acgcaaaata aaggtggtgg ccaatctccc ttacgaaatt tccagcctca tgatctttcg
    13261 cctgttgaag gaaagggaat gcttttccct tatggttttg atgctccagc tcgaggtggc
    13321 cagaagaatc gcggccaagc cggggaccaa ggactacggg cctctcggcg tctggtccca
    13381 gctttacagc gacgtgcaga tcgcctttca ggtaaaaccc aattccttta cccctccacc
    13441 gcaaatccag tcggcggtgg tgaagtttaa ggtccttccc aagccaagag tggaggtgga
    13501 ggaagaaaaa accctggaga gagttattcg ctccgcattt acctatcgcc ggaagaccct
    13561 gagcagggct ctgcagatgg gaggctttcc ccggcctcag gtccaggaag ccctgcgtac
    13621 atccggcatc cccaccgcca tccggggaga ggccctgagt ctggagaaat tctgcagcct
    13681 ggctcgtctt ttgggctcca agtgaagaaa agattcctcc tcctgtgttg tatcgtggcc
    13741 cttctggccg ttctctcagg gatttatggg gaacgggctt tccggagtat tctgtttttg
    13801 agcgaactct ttcatttcga taaacccgga tggacggaaa aggactcttc ccgcccgggg
    13861 ataaaagaag tttcctggga aggtcctcag ggaatcagca aagccgacct gtatctcccc
    13921 agccccgaag ggaaacgggg agggattctg gtcaatcatg gggtcatcga tacggggaag
    13981 gacgaccccc gcttgaagcg gtttgcgacg ggtctttgtc aggcgggttt tgtggtcatc
    14041 gttccggacc tcaaaggtat gaggtctttt cgtattgccc ctgcggatgt ggatgaaatt
    14101 cgggccgctt tttcccattt cctatcccgg ccggaggttc ggtcggattc ctgcggcctc
    14161 ttcggtttca gctatggggc aggcccaacg atcatcgccg cctgccagcc ggaagtccgc
    14221 gaaaaggtgc gtttcgtggt ttccttcggg gggtactacg acctgaagaa tgtcctctct
    14281 ttcatcgcca ccgggtattc tgagttccag ggaaaacgct attttcggaa acctcaggag
    14341 tacgggaaat gggtctttct ggccaataac ctcgattggg tcgagtctgg ggaagatcga
    14401 accgtcttga gaaagatcct aagaatcaag cttcgggatg aaaacgcgcc aatcgaccaa
    14461 tttttatctc agctgggtgg agagggaagg aacgttctga acctcctctc ccatgccgat
    14521 ccttctcaaa ctgaatcctt tgtccggaaa ttacccccgg caatccgctc ttccatggct
    14581 cacctctcgg tgtctcgcgt gttaaaggac ctccgggccc acctgattct ggcccatggg
    14641 gaagacgatg acttgatccc ctttactgaa actcttcggc tggcccaggc cgctccgcat
    14701 tcggaaaagg tgtgcatgag aatcctaaaa accttttccc acatagaccc tgaacaaaga
    14761 cccctgtccc cgggaaatct cttttctttt tacctaccgg aaagctggaa gctttttatg
    14821 ctcacctatc gactgcttgg ctaccgatga ccccaaattc ttgacacgaa agaggagata
    14881 ctggatactg gatatgggaa tacaggcgct ggtttctagg tccattaccc attgcttttt
    14941 tatccaatat ccagcaccca gtatccggga ttaagcatcc agggtctaac atggttgttt
    15001 tttgattatg agcaagctct accttcacct ccggaaacga tttgagcagg cggttctaaa
    15061 acacggaatg cttgcggaag gggaccgggt gttggtggga gtttcgggcg gagctgacag
    15121 cttgaccctt cttaagctga tcaccggccc ccttcttttc gtgcccaagc cagaattcgt
    15181 tctccccgtt tatgtggacc tgggattcga agggacgaac gggcagccgg cccgggtgat
    15241 gaaagattac ttcgaaaagg agggagggca ataccggatc gaaaaatcga atatcggcct
    15301 cttgtcccac agcgactaca accgcaaagc cagtccgtgc tttctctgct caaggttgcg
    15361 gcgccgaaag ctctttgaac tggcccgcga ttataaatgc aataaactgg ccctggctca
    15421 caataaggat gatgtcattg aaaccctcct gctgaatgtc ttttttggtc gggagatcag
    15481 caccatgatt ccctgccagt cctatttcca aggggagttc tatctcatcc ggcccttggc
    15541 ctacacggat gagtatctgc tgaagcgctt cgcccgggaa gcaggacttc ccgtgatgga
    15601 caacccctgt cctacggcca ataacaccaa aaggaagtac attaaagatt tgatcaaaaa
    15661 gctggatcaa gatcaccggg gggttaagga aaacatcttc aaggccatga tgcatgtgaa
    15721 gccggattat cttttaacca agtagcgatc agctttcagt atttagcggt cagcaaagaa
    15781 gctaagggag gagaaggttg ggggagcaat tgaaaatgct caatgctgag cgcttaatgc
    15841 tgacacttga cagcttcttt tctgttgaca acctgccgaa aatctaataa taaaaatata
    15901 cccttccagc atgtggcggt gtagctcagg tggtcagagc atgcggctca tatccgcagt
    15961 gtccggggtt caaatccctg caccgccacc cattcctcgc ggcgaatcca gtatcaaagt
    16021 catctttcgg cagggagaaa taagtttctt cctcagaggg aacctgatgc ttctgcacct
    16081 tgcagtcctg aaatttccaa taccatccat aattcccgtc caatggcctg ttggattccc
    16141 ggttgggtgg gttgggtctt tgggtattct ttattcttga aggcacggaa tgattatggt
    16201 atttatgggg agtgaaactt tcgggaggaa aggatgacta ggcgctacga tcctcagagc
    16261 acagaggaaa aatggcaaaa aatctgggaa gaacgggagc tcttcaaaag caacggcgac
    16321 ccggctcggc aaaaatatta cgttcttgag atgtttccct acccttcggg caaaatccac
    16381 atggggcatg tgcgcaatta ctccatcggc gatgtggtgg cccggtataa aaggatgcgt
    16441 ggctggaacg tgatccatcc catggggtgg gatgctttcg gcatgcccgc cgaaaacgcg
    16501 gccattcaac acggcgtcca tcccgcccag tggacctacg aaaacattgc agtcatgaaa
    16561 cggcagctgc gccggatggg attcagctat gactgggacc gcgaacttgc cacttgcgat
    16621 ccctcttact accgctggga acagtggctc ttcctgaaga tgtacgagaa gggcctggcc
    16681 tatcaaaaga cctccttcgt gaactggtgc gagaaatgcc agaccgttct ggccaacgag
    16741 caggtagaag cgggccgctg ctggcggtgc ggtgaggagg ttacccagaa agagatgccg
    16801 ggttggtttt tcaaaatcac ccactacacg gaggaactgc tggaatactg cgaaaagctt
    16861 cccggctggc ccgaacgggt catcaccatg cagaggaact ggatcggccg ctccgtggga
    16921 gccaccattc atttcccctt ttgggatcgg gaagggttta ttccggtctt tacgacccgg
    16981 caggacacgg tcttcggggc gaccttcatg accctggctc cggaacatcc cctcaccctg
    17041 gagctgtcca aaggaacgcc gcaggagaaa gaggtccggg aatttgtgga gcggatcaag
    17101 aagcaggaca aaatcaagcg gacctctgat gattacgaga aagaggggat gttcatcggg
    17161 gcctattgca ggaatccgct gaccggcggg aagatgccca tctttgccgg gaacttcgtc
    17221 ctcctggaat acgggaccgg cgcggtcatg gccgttccca cccatgatca gagggatttc
    17281 tactttgcca agaaatttgg ccttgatctg atcgtggtcg ttcagcctcc ggggaaacca
    17341 accctggatg cccaaaccat ggccgaggcc tacaccgaag acggagttct ggtcaactcg
    17401 gggcgattca acgggatgac cagcgaggag gcgagggaag cgatcgccca gtatctggaa
    17461 gagaaaggat cgggcaagaa ggccgtccaa taccgcctga gggactgggg gatttcccgg
    17521 caaaggtact ggggcgcccc gatcccggtc attttctgcc cccagtgcgg aaccgtgccc
    17581 gtgccggaga aagaccttcc ggtcattctc ccccaggatg taaaactcac cggattagga
    17641 gattccccgc tggcccacgc cccctctttc gtgaatgtgc cctgcccgcg gtgcggcaga
    17701 ccggcccggc gcgagacgga caccatggat accttcgtgg agtcctcctg gtatttcgag
    17761 cgcttctgca gcccccggga agaccgggga atgttcgacc gggaagaagt caagtactgg
    17821 atgcccgtgg accagtacat cggtgggatc gagcatgcca ttctccatct gctctactcc
    17881 cgtttttaca cgcgggtgct gcgggatttc cggctcttgg attttcccga gcccttcact
    17941 aatcttctca cccaggggat ggtggtgaaa gagacccacc actgttccaa ggacgggttt
    18001 ctctatcccg aggaggtgtc ggaaaacctg acctgccaaa agtgcgggtc tccggttcaa
    18061 gtgggccgga ccgagaagat gagcaagtcc aaaaaaaatg tggtcgatcc tgactttctg
    18121 gttcagaaat acggggccga caccgtgcgc ctcttctgtc tctttgcttc cccgccggag
    18181 aaagacctgg aatggagcga acaaggggtg gagggctcct tccgcttcct ccaccgggtc
    18241 tggcgcctgg tggaagactg ggcggatgtc gtgaaaacgg cgaaggctcg gcaaaaagag
    18301 gagctcctct ccgaagagct gagggccctg cggcgcaaga tccatgtgac cataaaaagg
    18361 gtaaccgagg atatcgagaa ccgcttccac ttcaacactg ccatcagcgc ggtcatggaa
    18421 ctggtgaact ccctctacca gatggaagag aagagaaaaa cgccgggtcc acagaccgca
    18481 ggggttatgc gggaggcggt ggaagcggtg gtcgtcctcc tctcgccctt tgtcccccac
    18541 atctgcgaag acctctggga atatctgggg cacgaacaaa gcgtggccaa ggaaccctgg
    18601 ccccagtttg acgtagaagc catccgggac gaggaagtcc tcatggtcat ccaggtaaat
    18661 ggaaagctgc gcagccgggt tacggtgggg gttcacgcct cggaggccga gatcaaagag
    18721 gcggttttgg ctaacgagcg ggtccaagaa attttgcagg ggcagaaggt gaggaagttt
    18781 gtctgggtgc ccaagaagct cgtgaatttg gtggtctgac ccaaaacagc agggagccgg
    18841 ggacctatgg ctggggcctc cctttcgccg accgcccgca gctctttgcc ccatgctaaa
    18901 ggagagtgcc catgaaaaga cgggtaggca ttctttgggg aattacggcc atcatcatcg
    18961 gaattgggat cgggggttgc atttacaccc cacaaaccaa aatcaccccg ccgatcacca
    19021 ttccctttaa gggtgaatac cggtttgaca agtcccttca ggaccggccg ccccaaacgg
    19081 tagcggtctt gcccttcctc aaccggacgg aacacaagca cgccttcgat atcgtccgca
    19141 aatcctttca tggccatttc agcacgctga attttaccgc cgtccccctg tacaagattg
    19201 attatgcgct caaccaggcg gggttggata cacccgagaa ggtggctgcg actcccgttt
    19261 ccaagttaag ggagatcgtt aaggcggatg ccctcgtcca gggagagatc acccattacg
    19321 accggattta catcggagtc tattcgcagg ttgcggtggg agcggaagtt cggatggtag
    19381 agacgaagac cgataaagag ctctggtggg ccaaggatgt ttcccggaaa cacgcggggg
    19441 ggatttccac cactccggtg gggattattc ttaccgcggt gagcacggcc ttaaacatgc
    19501 gggagatcga actcctgcgt tcctccgacg atcttttccg ggagatggtc cgaagcattc
    19561 cacagcctac ccttgcccag gccctgaggc ctccggccat tgccatcctg gcccatgacg
    19621 gcatgaggcg aaccgaccgg tacgcgctaa aggcgggaga tgtgctcaag gtggccctgg
    19681 aaggggaccc gcgaaaaaag gcctccttcc ggatcggcaa cttcaaaacg gatattccca
    19741 tgaaagagga agacccgggg atgtattcgg gaacctacaa agttcttccc ggcgattttg
    19801 tggaagaggc gctgataacc ggagtgttag cggatgacca aagaaattcc acggaatggg
    19861 tcgatgccct gggaccggtg accatcgata ccattcctcc agccgttccc ggagggatcc
    19921 ggggcattgg aagggataaa agggtggagt tggactgggc caaaagcccg gacaaggata
    19981 ttgccgccta caaggtctac cgcagtccta cccccttgac tgggtttcaa gaggtgggaa
    20041 agaccgaact gactctgttt gccgatgcga acctgaccaa tcagacgacc tactactatc
    20101 gagttgcggc catagacctg gcggggaatg aaagcaaacc atccgattcc atccgggcca
    20161 ccccaattcc tcccggcccc accccagtga gaggaacgat ccaaggagag gccatttggt
    20221 ttgccggggc cagtccttat atcctggaag acagggtttc cgtagagacg agagcaactc
    20281 tgaccattga gcctggaact gtgattcgct ccaaaggaga ggggctcgtg gtttccggaa
    20341 aactgctggc ccaaggagac cggagcgcca tgatctcctt tgaaccagtc tctccgggcg
    20401 gagaatggaa agggatcctt ttccaaggat cccagagtga agaaagcgcg gtgcggtatg
    20461 tgaagatctc cggcgctcag gccgggatca cgtgtctttc cgcatccccc ctgattctgg
    20521 gcaacgacct gtccaaaaac cgggtggggc tccggatcca ggaatctttt tccaaacctc
    20581 gagtccaggg gaacgtgatc tacgccaata cagcgggggt ggaaatcctg ggaggagcgg
    20641 cgccgattct ggaagagaac gagattcagg gaaaccagaa ggatggagtc ctggtccggg
    20701 aatccaatcc ttctctccaa aaaaaccgaa ttctgaacaa cggggaggcg ggggtgcgtc
    20761 tcttttcttc ctccgcccgg ctgacccaga acaacattca cgacaatggg aagtttgagg
    20821 tttttaacaa tcgggaaagg gaccttcccg tggaggctcc cgaaaactgg tgggggacca
    20881 aagaggggtc cagtctcgcc ggcaagattt atggaagggt ggagtaccaa cgggtgctcg
    20941 acgcgccttt tccccaggga aagccgatcg aacttcccct cttaaagagc cccttgaacg
    21001 ccccaatcca gaaggactcc ttcctgatct tggcctacag cccttatgtg gtggaacggg
    21061 aagtgatgat cgacggaggg gcgacgctct acattcagcc tggggtcacc ctgaaattta
    21121 atccgggggc atcgatggtg gtgaaaaatg ggggcatcga cgcccgggga acttccgatg
    21181 gggcgatcac ctttacctcc agtagctcct ctccttctcc aggggcctat cctgcggcga
    21241 tccgctttgc taaggagacc ggagtggcca gtttcttccg ttactgcatc atcgaatttg
    21301 ccgaaaccgg gatggaagtg gcctacgggt ctccagagat cgaccgctgc ttgatcgccc
    21361 ggagcgccca agctggtatc aaggtgaccc aagaaggtgc gccgaagatc tcctacagca
    21421 ccttcctgca gaatgcagga acgggggctt tggtggctct gggaggcgcc cgcccgaagg
    21481 tccatcggag caattttcgg gaaaacgatt tcgccgttca gagccattct tccatcttca
    21541 tggatgtccg ggagaactgg tggggggcga gcccgccctc cgaatcgatt ttcctgggac
    21601 agatgaactt gaagccctgg ctggaaaagc cggagccaga ggcatttggg gggaggaagc
    21661 cgtgaaggga ttcatttggg gtggcctcgt ttttttcttc ctggcgctag ggccaggaca
    21721 ggcctgtgcc caacctagag aagtgatcga gcaagtcggc aacggggtca ttaactggtc
    21781 gcaagggatt attttagcca aagggagcgg gtcccctccc aaggactcgc gaaatatcgc
    21841 ccaggcgcga ttgatgaccg aacgagcggc cttgaccgat gctcgccgca atctcctgga
    21901 ggtactcaaa ggggttcggg tggattcttc cactcgggtg gaaaatttcg tgaccaagga
    21961 cgatcagatt cgcctccagg cggaaggttt cattcagggg tcggtcgagg tgaggcgctt
    22021 tcgaaaatac ctttccgatg gagcgattga agtcacggtg gccattgacc tctccgggga
    22081 ttttatttcc ctcatgctct ctgcctccga ggaactgagg aagaaagcga caccccctcc
    22141 agccccgctg gtagaagcaa agcccgaaaa gaaaactctg ccacccccgt cccctcctgc
    22201 tgggccagcg gtacagctgg ccccgcccca ggaaaagaaa cctgagctgc ctccttctgc
    22261 gccgcccatg gttaaggaag taaagccgat ccctccaccg ccggtgcctg cccctgaaag
    22321 gaaaccggag cctcctccgc ctccggctaa ggtgggggaa gcccctctgc cagacccggc
    22381 gaccttgccc ccgttcacgg gggtggtcat cgacactcgg ggtctaaaaa tgaaacccgc
    22441 cctagccatt cgcattctaa atgaaggcgc gaaggaatta taccgagggg aatatgttcc
    22501 ccaggataag gccgcccgga acgggttggc cctctttgcc agggatttga ccgccgctca
    22561 aaccaatccg cgagtgggga aaaatcccct aactatcaaa gggtccaagc tcgacccggc
    22621 cgctccttcg gatatcatcc tggatcaagg ggaagcccaa aagatggctc cctttgccca
    22681 aaaagggact ttcctggagg aatgccgggt gatgatcgtg ctggactgag gaggcccctt
    22741 gagaaggctg agtctgggca tcttccttgg ctttctctgt ctggccggat gtggatacca
    22801 gctccgcggg agagagacca acctccctcc cggcatttat tcggtggccg tccccatctt
    22861 tgccaaccgg accgatcaaa ccggaattga gacagaggtt acccgggccc tggtcgaaaa
    22921 atttatatcc accaagcgcc tctcggtagg ccctcggaat tcggccgatt cccttttaac
    22981 cggaacggtt aaatctttca ccaccttccc ggtggccgtc gttagctcga cccagtcagc
    23041 caccgaatat cgggctacgg tgatcctgga gtacagcttt caggggcaga aagacggaaa
    23101 ggtgctcttc cgggaagaaa taagcgagtg gcgaaattat ccggtggtct ccgatctgaa
    23161 cgtcaccgag cagaataaac tggatgccat ccggagtatc tccgttctcc tggctgaaag
    23221 aatccatgaa caaattctgg gcggtttctg atgcgaccca gaatccctgc ctcctacgac
    23281 caaaggacat gaaggcatcc gaatttttcc gcgaaatcgg cagagggaac ctgctccccc
    23341 tctattattt ctggggtccc gagaaatggc tgatcgatga tgccctgaag aggatcgagg
    23401 atcgggccct ttctcccatc acccgcgact tcaaccggga agcgttcgac gccgaagagg
    23461 ccgagaccga gtcaattctg tccagcctcc aggcttttcc ggtgcggtct gcctggcgac
    23521 tcgtgatcat ccgaaaggcg gatgaaatct ggaaaagaac cccttccccc ttcgtcgatt
    23581 actttcagaa ccccaaccct caaacctgcg ccgttttcat tggggctcgg gcggaccttc
    23641 gggctaaatt tttccagtcc ctggagagga agggggccgt ggtccctttt taccctccct
    23701 ttgagaggga cctggtccgc tgggtccatc tccaagccga acagctgggt cacaacgtat
    23761 cagacgaggc tgtgaccctg ctggtcgaaa gggtgggccc aagcctgcgg gagattctgg
    23821 tggaacttca gaagctgagc ctgaggaaag aaaaaggccg ggggatcacc gaggaggatg
    23881 ttttcgcgct gaccgcagac tgccggccgg agagtccttt cgaatttccc caggcagtgg
    23941 gtctcctaaa gagggaggag gccctccgcc ttctccacaa gaacctccag cagggagagc
    24001 ctccgatcct ccttttttcg ttgctggtgc gccagcttcg cctgattcag agggcgcagg
    24061 agatgcgggg cagggggctg accaaaaaag aagtcgaggg gaagttgaga attccacccc
    24121 ggagtgcgga agagttctgg gcccaggtgg agatatttcc ctcccccatc cgggaagggc
    24181 tttggccggc cacctggagg accgaccaga agatgaaatt aagccggggg gacaaggggc
    24241 tcctcttgga ggaatatgtt ttgaggcttt tgggagaccg ggcggagagc cagcagccgc
    24301 tcaggccccc tctccgccgg gaaaaggggg aggaatgaat tcaagcccca gagggaaggg
    24361 aattcacttt tttggccagc atggagattt tccgggcggc ggtcttttta tggatcacct
    24421 ttttcccggc agctttctga aaggcgggaa tggcgctgga aagagccttg cgggcatttt
    24481 ctgggttctt ttgatcgatg gcctccagga cctttttggt aatggtcttc agggcggatt
    24541 tgactgagga gttgcgctgt cttcttctct cgctctggcg cgcccttttc agggcggaat
    24601 cgtgagtggc caagggacct ccttgaatag gaataaagat ataatctatt aaaaattaga
    24661 tataaaagaa agtcaaggtt tttccgggtc cctccggctc tccaaagcgc caatctccag
    24721 ggccgagccc catttccttt tcaaggtccc aacgatccaa gatccgggtg gcgaggttta
    24781 caggttagaa ttcgggatcg ggtttattta agaaaatttt gcgaccgggg tatcggcaaa
    24841 accaaaggaa tggagaccca gcgaagaata accgaggcgg caagtgttgt gggtggagcc
    24901 acgctccttt cccggatttt cggctttctc cgggacatga tcgtggccca actcttcggg
    24961 gcgggcaccg ccactgacgc cttttttgtg gcctttcgca ttcccaacct tcttcgccgt
    25021 ttggtgggag agggttcgct gaccgtttct ttcgtcccgg tctattcttc ctacctgacc
    25081 cagaaatcga aggaagagag cgaggaattg gtgagcgttt gcttcacggt tctggctttt
    25141 ttcctgcttc tgctgaccgc cgtgggaatt tttttttctc cctgggtggt aaagatcatc
    25201 gcctacggtt tctcccagga cccggagaag ttccggctga cggttcttct tacccgggtg
    25261 atgttcccct acattttttt cattggcctg gtggcctggg ccatgggggt tctgaatgcg
    25321 cgaaagcatt ttgctggccc agccctggcc cccgtcctgc tgaatcttgc gatcatcgct
    25381 tgcgctattc tgctctcggc ccatttgggc gaaccggtta tggctctggc tgtgggagtt
    25441 ctcctggggg gagtcgccca gctacttttt caagtccctt ttctccggcg gcaggaaatc
    25501 cgcctgaagc tgcgtttcga cctctcccac tccgggatga ggagggtggg ggctctgatg
    25561 gctccctcga tcctgggttt ggcggtcacc cagctgaatg tgatcgtcaa cacctttttg
    25621 gcctcctatc ttcccgaggg aagtgtttct tatctctatt acgctgaccg cctcctggaa
    25681 tttcccatgg gggtcttcgc catcgctatc gccacggccg tactaccgtc cctatcagag
    25741 caagcggcca aggggaaaat agaggaagtg aaggagactc tatcttttgc cctccgcctg
    25801 acctttttcg tcatccttcc ggcgatggtg ggattggtcg tcctgcgaac ccccatcctt
    25861 aaccttctgt tccaaagggg agcgttttcc gccttttcca ccgaaatgac cgcccaagcg
    25921 cttctctatt atgccctggg attggtcgcc tttgccgggg tgcgcatcat cgcccctgtt
    25981 ttttattccc tgcaagacac ccagactccg gtcaaggtgg cctttgtgag cttgctctcc
    26041 aacgcagcct tgggagcgat cctcatgttc cccttgaaac acggaggact tgccctggcg
    26101 acttctctag ccgcaggagt gaactgtgcc ctcctggcct accttctgcg gaaaaaaata
    26161 ggccccatgg atatccgtcg catccttcat tcctttgcca agaatatgac cgcctccatc
    26221 ctcatggggg tgacggccta tgccatcgtc tatcccggcc cctgggaaac ctcgggaatc
    26281 acagcagaga aaatgaccct tcttggcgcg gccgtaggag ggggtgtttt ggtctatgga
    26341 acagtttcct atttcctggg cggcgatgaa atgagggcgg ctatgaaact ggccaagaaa
    26401 aaggccgggc gatttttatg atgcgagggc tgaaaaggac ccggctctcc gtttcttttg
    26461 tcctccgggg gaacccttgc catctccggt gaaagggcct cctctccttg caggaaatct
    26521 tcctgagtta ccctcaaccc ttcctactgg tcggcctaaa acaaaacttg aaaaattgcg
    26581 acaacatgtc aataataata aaagatttgg atatttttga aggttttgtc atttcttggg
    26641 gcatgggatg ggtgaatttg acaagatggc accggctgat gaagagaaaa attcctttcc
    26701 ttcaagggga tgggaaacaa aaaaaccaag aaattcaaaa tcttgttttc catgaagaag
    26761 gaggattcgg gtatatctgg catcttcttt gctataaaaa gattccttga atgagtatac
    26821 ccggatggaa aaagcatatt gattcggggg aatcacttct gctgcccaaa ggttctttaa
    26881 attgggtagg ccatttccat ttagtggcgt tcagtatact tcatagagga ctatgaatcc
    26941 ccgcttatga ttgccgaatt cgagattcag gggcaggatt tcaagagagc aggtgaggct
    27001 tgttttgaaa tcaagaaccg gctgaagcgt ttggggcttc cgccgggtat cattcgccgc
    27061 gtagccaccg cggcctatga agctgaagtg aatgtcattg tttacgcgca caaggggagc
    27121 atccgggtga atcttgatga tcagtgcgtg gaggtgacgg tctgcgacgt ggggccaggg
    27181 atcccggata ttgagcaggc gatgcaagtg ggctattcca cggccccccc ttacgtgaaa
    27241 cagctcggct atgggagcgg gatggggctt ccgaacatca aaaaaaattc cgattggatg
    27301 gaaatccaat cggaattgaa ccggggaacc accctgaggt tcaaagtcaa tctgaatgaa
    27361 agtccccaaa aggatccttg aagaccccgg ccgggccggg ggagtttttc tctcgctgaa
    27421 ggaagaccac tgcgtgggtt gtacccattg tctccgggta tgcccgacag aagccatccg
    27481 catccggggg gggaaagccc ggatgatgcc ccaccgctgc atcgattgcg gcgaatgcat
    27541 tcgggcttgc ccccggaaag cttggacggt cgaggccaag ccctgggaaa gggtcctaga
    27601 aaagggcaaa atcgtggccg tcctggaccc ggcggtattg gggcaatttg gagatcgggt
    27661 tgttcctgga atgatcctgc ggactttctc ggaaatcggt ttttccaaag tggaggatat
    27721 aggccaaggg ctagccgttt atcgccgagc cgtagctggt catttagcct cggggcgggc
    27781 gccttctccg gccatttctt cggactgccc cgcggtggtc caactggtgc aagtcaagtt
    27841 cccgtccctc ctggaaaatc tagttcccgt ggtccccccc tttgaaatca tggccgacct
    27901 gctcaggagg aataccgaat ggaggtcgac cggtggcctg ggctacgtcg ttccctgtct
    27961 ggccaaggcc cgggcagcga ccgaggctgg caaaccggag cggtcctacc accatgccat
    28021 tccgttcacg gaggtttatc ctatcctgcg cgctcagttg cacgggagag aagaagagcc
    28081 tttcccggag gcgggggggg ggccttccgt ttcggccctc gagtgggcct atcccggtgg
    28141 acagagtcgg gctatgggcc tggaggcttc cctcatcgtg gatggagtga gccaggtggc
    28201 cgaggtcctg gaacttgcgg agaatgggct gctggaggaa gtgcctttca tcgaagcctg
    28261 gagctgccgg ggagggtgcc tgggaggtcc gctcaatgtg cgcaacccat tctgggcccg
    28321 gtttcagctt ttctcctgga ttcgaaagga cgacgcccgg agtccggaaa cccttggtcg
    28381 gggggaagat gtgaacgctt atatcatgga tcaacccttt cctccaaggg ccgggatgcg
    28441 gttggatgaa gaccttcaag tggccatgga aaaacttcgc cgcatcgaca aagttgtcaa
    28501 gcagtttccc gggatcgact gcggttcctg cgggtccccc agttgccttg ccctggcgga
    28561 agacgtggtc cagggatatg cccgggagac ggactgcatt tgcattctga aaaaggggaa
    28621 aaccgttgaa gctggaccgg gaaaagaggc ttcaaaggga aggccgccaa agtcaagagg
    28681 gaaacggagg tgaaagggtt ctcccattcc gaccatgaaa ctgaaagaac tggcagagag
    28741 actgaacctg gaactgacca cgcccaatgc cccgatggaa gccgaagtga agaccggata
    28801 ctcctccgat ctcctgagtg atgtcctggc caactccatc gagggggatt tatgggtaac
    28861 ccggcagact cacctgaaca tcgtggccat tgcggtcatg cgggacctct cgggaatcct
    28921 gctggtcaac gggtcccagc cggacccgga taccctggag aaagccgcag agaagatgat
    28981 tcccatcttc cggaccgccc ttcccacgtt cgaaatcgtg gggcgtctgt accaactcgg
    29041 gatctccgga cagcgctgaa acatgctcga gctttccatg cacattctgg atattgctca
    29101 gaattccctg cgggcgcagg ccaagggagt ggagattcgg gtccaggaag acctggggaa
    29161 ggacctcttg cggatcgagg tgctggacga cggccggggc atgtccccgg aaaccgtcaa
    29221 gaaggtgacc gaccccttct acacgactcg cccctcccgg gtggccggtt tgggggtgcc
    29281 ctttttgaag gaggccgcgg aacaatgcgg cggaggcctt gaagtccatt ctcgggaagg
    29341 caaggggacg cggatcgtcg ccaccttcca gctggatcac ttcgatcggg cccctctggg
    29401 cgacatggga gagaccttgg ccatcctgat cactggaaat ccgggagtgg aatttctcta
    29461 cgagcatcgg gtgaacggtc ggacttaccg gctggacacc cgggagatgc gggagatcct
    29521 ggggccagtg ggattggggg acccttcggt cttcgccttc atccgtcagg atgttcagca
    29581 aggcctgaaa gcgatcgggg ccgcgacttt tccccaaacc atgaaccttc tggggggacg
    29641 ggaaaaccca tgacggtgat atccacgatc aaagaaaaat gccgccgctg ctacacctgc
    29701 gttcgaaaat gcccggccaa ggccatcaaa gtggaagacg gccaggccaa ggttgtggcc
    29761 gagaggtgca tcgcctgcgg gtcctgcatc aaggtttgcc ggcaggaagc caaggcaatc
    29821 cgtgactcca ccggcacggt gaaggacctt ttttggaatt ctcccgagat catcgcctgt
    29881 ctggcccctt ccttccccgc agccttcccc caggcctcgc cgggccaaat cattactgcg
    29941 gtccgaaacc tgggttttac cggggtcatg gaggtagcct tcggggcgga cctggtggcc
    30001 aaagcccatg cccgctggac gaaacgtttc gccgacaagc cctacatctc ctctccctgt
    30061 ccggccttga ttctctacat caagaaatat ttcccccctt tggtagacca tctggtcccg
    30121 atcgtctctc ccatggtggc tatgggaagg gtgatcaagc ggaatattcg gccggaggcc
    30181 aaagtggtct tcatcggccc ctgcatcgcc aaaaaggaag agatcgaaga cccagaggtg
    30241 gccggcgatg tagacgccgt gctgaccttt gtggaattgg accggatgtt tcaggaagca
    30301 ggcttgatcc cggagaagct ggcggagagt cccatcgacg gtcccctgcc ccggatggga
    30361 cgcatcttcc ccgtgcccgg gggattgctg cgcagcgcct ccatgaagga ggacgtctgc
    30421 cagaaccgga ttctggtcac cgaaggaaaa gaggcctgca cccaaatcct gaatgaaatc
    30481 ctggaggggt ccgtggaagc gaaattcctg gaccttctct tctgcgaagg gtgcatcaac
    30541 gggccgggct tccccaatga gctttcggtt ttttcccgca aagaactggt ggccaactat
    30601 gttcaatccc gcctccgggc ggagagggaa gaccgccaca gggccgacat gcgcaagcac
    30661 tcccgtattt ccctggggcg ctccttcagc gccgaggacc ggaggttgac gacccccagt
    30721 ccggacatca tccgggaaat cctgcggcgg accaataaag ttcacccgga ggatgaactg
    30781 aactgcgggg cctgcgggta cgcctcctgc caggaaaaag ccagcgccgt ttactgggga
    30841 ctggcggaaa acgaaatgtg ccttccctac ctcatcgatc agctggagga aaacctgtcc
    30901 cgcctggccc agtcccataa ggaccttcgg gaaacccagc agcagctgat ccagtcggag
    30961 aagatggctt cggtgggtca actggccgcg ggcgtggccc atgagatcaa taaccccctg
    31021 ggaacgattc tcctctattc ccacatgatc ctggagaaac tggagcagaa ggacagccgg
    31081 cgggaggagc tggacaccat cgccaaggag gcgacccgct gccgggacat tgtccgcggc
    31141 ctcctggatt tcgcccgcca gaggaagctc caggtggaga atgtggatgt aaataaaatc
    31201 ctggaagagg ggctctccct ggtggcggcc cagcccgctt ttcagaaggt ggagatcgcc
    31261 aagaccctgg acccttccct gccggccacc gaaggagacc cggtgcagct gaaggaggta
    31321 ttcctgaaca tcctctccaa tgcgggcgag gccatgcccg agggcgggag ggtgacggtc
    31381 gtctcgcgat tccgggaagc cgggtctcac ccgatcgaga tcatgatccg ggacacgggc
    31441 caggggattc cgcaggagaa tctgaataag ctttttatgc ctttcttcac caccaagaga
    31501 atcggccaag ggaccggcct gggcctggcc attgcctacg gaatcgtgaa gatgcaccgg
    31561 ggagccatcg aagtgcagag caaagtgggg gaagggacta cgttctgggt taagcttccg
    31621 gcggaatctt ccctaagaag tcaggaatcg gtcggttgaa gaaccgggtt ttttccggct
    31681 gccgggaagg aggacgaggt tcgtatgaaa gggagcccga aggtcttgat cgtggatgat
    31741 gaccctgatt tcatcgaaat cggaaagctc tccctggaag ggaagggata tcaggttctt
    31801 tctgcatcca ccggaagaga aggatgggag atggtggaga aggaaatccc cgacctggtc
    31861 atcctggacc tgatgatgga ggacttagat gccgggatga ccctcagcag ccggattaaa
    31921 agtcaccccc ggtatgcgag catccccatt ttgatgctca cctccatctc ccgcgagacg
    31981 gggatggact tcactcctcg caccgaggaa gaccttcgga agttgaaggt ggatgatttt
    32041 cattccaagc ccatcaaggt caaggtcctt caggaaaaag tagagaaatt gctgagccgc
    32101 ggatcaccat ctgggagccg atgaaaggag cgggtggatg aagggcgcca gaatcctcat
    32161 tgtcgatgat gaaccgccca tccgggccgc ttgcgccaag atcctatcgg agcagggggc
    32221 cttggttgaa acggcggaag acggcctggc cggcctggag aaggccagga cccaggagtt
    32281 cgatctggcg ctgatcgatc tgaagatgcc ccagatggac gggatgcagc tgctggaaca
    32341 cctgaaccaa ctcgaccccg acctggtcaa gatcgtgatt accggctttg ccaccctgga
    32401 tacggccatt gaggcggtgc agaaaggggc ctatgattat ctccccaagc ccttcacccc
    32461 gggagagatg agaacgcgag tgcgacgggg ggtggaaaaa cggaccctcc tgcgggaagc
    32521 caagaggcta cgggaggagc gggaaagaaa ccttctggag ctgaccaatg agaaaagccg
    32581 gacccaaacc atcatccagt gcatgggaga tggccttctg gtcactaacc gcgaccagca
    32641 ggtggttctg tgcaaccccg ccgcccggcg tctcctaaag atcaagcgtc ccctgcaggg
    32701 aggcgagccg ctgagtaacg tcgccggctc tgcggagttc ctcgacctgg tggagggcac
    32761 aatgggtctc aaagaaggcc aggagatgag ctcccgggag gtcgcgcccg cctctccttc
    32821 cggtccggtg ctcatggcca actgcgctcc ggtgaaggat gaaaaagggc agatcatcgg
    32881 agtcgtaact gtcctgcggg atattaccga gctgaaggct ttggataaag ccaaatccac
    32941 ttttgtctcc ctggtggccc acgaactgca ggcgcccctg gcggcgatcg aagggtatct
    33001 ggacattatc ctggacggcg gaggggggaa ggatcccgaa aagacccgca agattttgga
    33061 ccgatgtcgg gaacgcacca gcggactcct cgccctgatc caggacctct tggccatttc
    33121 ccgcatgcag agcgggcgaa tcgttcggga aaaggaaaaa ctgcggttga cggatgttct
    33181 gaaagaggtc gtggagttga tgaaggggga ggcgctggag cgaggggtga agatcgcggc
    33241 ggaactgccc gaagaattac cccccatctc cgccaaccgg gaggacctga cccgggtctt
    33301 caccaaccta atggacaatg ccatcaaata caatcctgcg ggcggacgag tcttcctccg
    33361 ggcgaaggcc ctggatgcct tccttcaggt cgaggtccag gatacgggga tcgggattcc
    33421 tgctgaggaa aaggagaaaa tcttcgacga gttttaccgg gtgaagaaca aggacaccaa
    33481 gggaattggg ggaacggggt tgggactttc gattgccaaa aagatcctgg aagcgcacaa
    33541 cggacatctt gaggtggaga gccaattgca tgagggaagc acctttcggg tgcttttgcc
    33601 cctgtgatgg ggtataatca aaaaaatgtt gctggttgct cgttcctggt tgcttattgg
    33661 agataaacct ccctttgagg aaccaccaag ccgaaacgag cagccagaac cagcaacgag
    33721 caaccaacaa tgatttttat cgccctgcga gggaggcgtt ggctgacctc agggggatcg
    33781 ggagtaggat gctagggaga aagcccagtc ccccccgggg gcctgggctt ttttttgggg
    33841 gaaagaatcg gaatggaaag aagattggga gtggttggaa tcgtgattga agaccggaag
    33901 aaatcggccc ctcaggtcaa tcaggtcctc agcgagcacg gcgaaatcat cgctggaaga
    33961 atgggattac cctacggccg acgaaacgta tcggttattg ccctgatcgt cgacggcacg
    34021 acagatgaac ttgggtcttt ggccggaaaa ctgggcatgc tgaatggggt tcaagtctgt
    34081 tcttctttat tccgccgatg atccgcaatt cgcgaaatgc tcttaaaagg tagaaagaga
    34141 tggctgaaaa ggcaaaaatc atagatgagg aacggattca aaggaacctt gaagcgacaa
    34201 aggggaagtc ccgcgaagag gtcttggaaa tattggccaa ggccgaggaa gccaagggac
    34261 tggagcaggc agaggctgcg gccttgctcc aaatggaaga ccccgccctc ctgcaagaga
    34321 tttttgaaac cgcccgcaga gtcaaactga agatttacgg caaacggctg gtcctcttcg
    34381 cccccctata tttgagcaat cattgcgtga acaactgctc ctactgcgga ttccgccggg
    34441 acaaccggct gattccccga aagcggttaa cccgggcgga gattcgggcc caggtatcag
    34501 aattggagaa gctggggcac aagcgactcc ttctggaatg cggggaatcc tccctttcgg
    34561 acatggacta tgtggaagaa gccatcgcca cgatctacgc cacaaaaacc gaaaaaggcg
    34621 aggtccgaag ggtaaatgcg aatattgccg ccaccacggt ggaaaactat cgccgcctga
    34681 aggcggctaa gatcggaacc tatcagcttt tccaggaaac ctaccaccgg gccacttacc
    34741 tggaaataca cgatggcccc aaacgggatt atgagcggca aatcctggcc ctcgatcgag
    34801 cccaggaagc gggaatcgac gacgtggggc tgggcgtcct tttcggcctc catgactaca
    34861 agttcgaaat cctggcgatt ctttcccatg cccggcattt ggaagagaga tttggcgtgg
    34921 gaccgcacac catttctgtg ccccgcttcc gcgcagcccg gggggtaagt tttctccccc
    34981 ctcatccggt cacggaccag gatttcaaga gactggtggc tgtccttcga ctctgcctgc
    35041 cctacacagg gataattatt tccacgcggg aaagcatggc gatgaggagc gaggctttct
    35101 ccctgggtat ttcccagacg agcgcgggct cgcgaacctc ccccggcggg tacggagcca
    35161 aaggggaagg gaacgaagag gcgggacccg gcgcgcagtt tgagaccgcc gatcatcggc
    35221 ttccggacga agtgatacgc tccatctgcg aggagggata cctgcccagt ttttgcaccg
    35281 cctgctaccg ggtggggagg accggaaagg acttcatggc cgtggccaag cccggggata
    35341 tccaacattt gtgcgggccc aatgcgattc tcacctttaa agaatacctg ctggactatg
    35401 cccccgagga aacccgggaa gtgggcgaga tagctctgaa gcagcatctg gaggacattt
    35461 ccaatcagag cttgaaagag aaaacccggg aacgactgga agagatggag aacggaaaga
    35521 gggacctata cttttgagtt caccaatagc agagggcgga gcgatatttt aaggaacgct
    35581 tggcgccgtg ctctgtgggt tttaccaagg cgacatgatt cgacccggat tcctggagac
    35641 ccttgaagcc ggagaaaagg gaaaagtcct ttcttcggga gaaatggagt tgcttctttc
    35701 ggcggacccc gaagaaagag aggccctttt ccaggcggcg gaccgggttc gtcgacgttt
    35761 cgtgggcgaa gaaatacacc tgcggggaat tgtagaattc tccaacatat gcgttcaaaa
    35821 ctgtctgtac tgtggactga ggaaggataa ccgagacctc cgccgttacc ggatgaccct
    35881 tggggaggtc caggaatcgg ccaaggcaat ccaaggcatg gggatcaaga ccctggttct
    35941 ccaatccggg gaagacccat ggtacacgcg ggaccgcctc tctgaactga tcaggagaat
    36001 caaagaagaa accgggctga ccctcacctt atccgtgggg gaacgccctc gagaggacta
    36061 ccaggcctgg aaggacgccg gggccgatcg ttatcttttg aagcaggaga cgagtgcgga
    36121 gcagctcttt gcttatcttc ggccggggcg caccctgacg gagaggttgg agtcgctgag
    36181 gtttttgaga gagctgggtt atgaggtcgg ctcggggaac atggtcggac tgcccggaca
    36241 gagccataac gatttggtcc gggacattca gctcttccgg gagttcgatt ttgatatgat
    36301 cggaatcggg cccttcattc cccatcccca gactcccctg gggaattcgg ccaccccgga
    36361 tttgaacctg accctgaagg ttctggcttt gacccgcatc gtcacccgtg atccccatct
    36421 gcccgccaca accgcctccg cggttctgga acctgaggga agaaaaaggg ctcttttggc
    36481 cggtgccaac gtgatcatgc cgaacctcac tccctggcaa taccgcggat actaccaggt
    36541 ctatccagga aaagaaaaac cggatggagg attggattca actctaagaa ccatatcctc
    36601 tttgggtcgg accatcagcc agggacccgg gtatcgccgc tcccttccgg ggaagcggag
    36661 cagttgaacc cgttctaaag gacccaccga accactgccc ctttttctcc caagggaaat
    36721 aggaacagga acgggagaac ggcttatctc taattttttt gcgtgaaggg gatacatgtc
    36781 attctgctga cttaaaggga agaaattcat aggtcccttg actttttcta gaaattaaat
    36841 atcataatat ttaggacact caggaaaagt tttcggaaga ggggaaacgg ctggacggga
    36901 aaaggtcaag aagatggaca agttcgacat cctggaggag aaaatcaatc aattggtgga
    36961 tgcctactcc gctttgcgcc gcgaaaaaga ttctttggga gcgaggctca ttgagaagga
    37021 atcggaaatt cacaaactca atgaaaggat caaccagttt tcccgggagc gggacatggc
    37081 ccgggaaaaa gtggaaggtc tcctacaccg agtggaacgc ctgattgctt cccctgcggg
    37141 tatgggaaaa ggatgaaaaa cctcgtccgg gtagagattt tgggccggga atacacgatc
    37201 aagagcgatg agggagagga acgggttaag aaaattgccc agtatgtgaa tgccaagttg
    37261 aagaagatat cggagagcgc tcaaaccctg tctactcaca acgcagccat cctggcggcc
    37321 ttagacattg ccaacgatta ttttgaagtt ctggagggac gcacctccct gagccagaag
    37381 gtggagaaga tggagaagca aacggggagg ctcatcgaaa taatcgattc gaagatccat
    37441 taaccgcgcc gaaggaacga tcccctgcgg tgttggtgat gatgctacga ttctttgagc
    37501 caacagcgaa aatacgggag ctgtccctta ttgtggctgg cagggcctga tccttcggga
    37561 gtacaggaaa gccaaagaca ataatatggc acccacctag acatcctagg ttcaagaaga
    37621 tggcatcaca cggcattcgc gggggttccc atcggaggag gggtttcggt ctccatcggg
    37681 gtggagattg cttcacattg ccagttgaat gggagaaaga agaggggagt caaggaatga
    37741 aattcggccc aggccgttgt ggtaaggacc cagaggaaga cccgatcctg ttttgtgggc
    37801 gaaagaaacg ctctggattt ttctccaatt ggggggcatg atctggggaa accttcggtc
    37861 ccctccgaga tcatgcgaga tattcctcgg tttctcattt cttccttcga ttttctttcc
    37921 ctatctattg aactttcttt ccgtgtaggt ccagtcgagg gacctcggaa gattttcctg
    37981 atcaagaaaa gggtttagaa ccgtgaacga atatttctat tggatcatcg gattgggttt
    38041 ggtccttctg gcggctggcc ttttgatcgg tttcttcctc caccaaaagc ttgccgcagc
    38101 aaggattcgt ctggccgagc agaacacccg cgaagtggtg gaagaagcga agaagaaggc
    38161 cgaggccatg aggagggaag cggaaattca agccaaggac ttccttttcc aggccaagac
    38221 ggaatttgag gctgaagtca aggaccgccg ccaggaaatc gggaacctgg agaagcgatt
    38281 cctgcagaaa gaggagaacc tggagaagag gatcgacctc tttgaacaga aggaagccaa
    38341 tctttccaag cgcgagaaat ccatcctcca gcaggaaaag tccgttggcg atatggaaaa
    38401 gaaatatcag tccctcctgg gcgagcagcg gcaactgctc gagcggattg cggggatctc
    38461 cagcgatgaa gccaaggacc gcctcatgaa gatggtggaa agcgagatgc gccacgagtc
    38521 tgccaagctc atcaaacgga tcgaggagga gacccagggt caggcggaga aaaaagcgcg
    38581 ggagatcatc ggaaatgcca tccagcgcta cgcggccgat tatgtggcgg aagagaccgt
    38641 ttcggtggtg aaccttccca acgaagaaat gaagggaaga atcatcggga gagagggacg
    38701 gaatatccgg gccctggagg cggcctgcgg agtggacctg atcgtggacg atacccccga
    38761 ggcggtgatt atctccggct tcaaccccgt ccggcgggag atcgcccgca tcaccctgga
    38821 acgattgatt accgatggcc ggatccatcc tggaagaatc gaagaaattg tggccaaagc
    38881 cggggaggag gtcgagaagg ccatccggga aggaggggag cgggccacct tcgacgtggg
    38941 agttcatggg atcaatccgg aactgatcaa gatgatcgga aagctgaaat accggaccag
    39001 cttcgcccaa aatgtgtacc agcattccct ggaggtggcc tttctggcgg gggctatggc
    39061 ctcggaactg ggggtcaatg tgaaacaggc caagcgggct ggcctcctcc atgatattgg
    39121 aaaagcggtg gatcacgaag tggaagggtc ccacgctctg atcggggccg acctagctcg
    39181 aaaattcgga gaaggacaaa agattgtgca tgccatcgcc gcccatcatg acgatgaaaa
    39241 acctgccggc ctgctggata ttttaatcca agcagccgac gccttgtccg ccgcccggcc
    39301 cggcgcccga tgggaaatgc tggagactta tgtcaaacgt cttgaggatt tggaaagaat
    39361 tgcgttatca tttccagggg ttggtaaaac atacgcaatc caggccgggc gagaaatccg
    39421 ggtgatcgtg gaaaatgatg agatttcaga cgccgactcg gttatgctga gccgggatat
    39481 tgcccgaaag atcgaaaatg aactgagcta tcctgggcaa atcaaagtga cagtcatccg
    39541 tgagactcgg gccatcgaat atgctaagta attgaaatta aataaaaata atttaggcga
    39601 agaaaatttt tgggaatgct cttttttgtt gacaaaaggg attgaggagt ttagattatt
    39661 acttaagtaa ggggacggaa aagttccgca agaatttttt tctgctcaat aattttccct
    39721 tgacaaccga ttaatagcct gcaataatta aaatcttcga ccttaaacct tgttctttga
    39781 aaactaaata gcaaagtcaa tgccgttggg tccgctcttt tgttttacct ccagggcctt
    39841 cgggacctgg atgaacaatt taactggaga gtttgatcct ggctcagaac gaacgctagc
    39901 ggcgtgccta acacatgcaa gtcgcacgag aaatcccggg caactgggag agtaaagtgg
    39961 cgcacgggtg agtagcgcgt gggtaatcta ccctgaagtt gggaataaca cctcgaaagg
    40021 ggtgctaata ccgaataatg tccccgggat tctgatcttg gggatcaaag atggctgagg
    40081 cttgcaagct atcgctttag gatgagcccg cgtaccatta gctagttggt ggggtaatgg
    40141 cccaccaagg ccatgatggt tagctggtct gagaggatgg ccagccacac tggaactgag
    40201 atacggtcca gactcctacg ggaggcagca gtgaggaatc ttgcgcaatg gggg
//
LOCUS       DASPRR010000156         5147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_195191, whole genome shotgun sequence.
ACCESSION   DASPRR010000156 DASPRR010000000
VERSION     DASPRR010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195191"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1421)
                     /locus_tag="VEL68_06175"
     CDS             complement(198..1421)
                     /locus_tag="VEL68_06175"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXZ35585.1"
                     /translation="METFIARQPIFDAQRRVYGYELFFRSGLENVFKHSDPSQATSKV
                     MVDSFFLFNLDDLTGGTRAFINVPRDILLKEYMFFLPKDQVVVELLETVEPDPEVLQA
                     CQKLKRAGYLIAVDDFVYEDRYEPLLEFTDFVKVDFLSTPELARKALFEKVSPYKVRL
                     VAEKVETHQMFEEGIESRYSFFQGYFFSKPAIIVGKDIPGFKAHYFQLLKEIHQAEID
                     LNRLEEIIKQEVAFTYKLLRYINSAFFGLRSKITSVKQAMILLGERTIKNWITFVALA
                     SMGADKAEELLIMTIVRARFCEMLAPYFDLSDRKEDSFLMGLFSLIDAFLDRPLSAIL
                     AEIPIDEPIKLALLGEPNRLRDIYQYTLSYEKAAWGHLEKPMIPPGEETTPFSLYLEA
                     LKWGQAFYSETKGMP"
     gene            complement(1500..3623)
                     /locus_tag="VEL68_06180"
     CDS             complement(1500..3623)
                     /locus_tag="VEL68_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXZ35586.1"
                     /translation="MSSLDRRQFLKAMGGGLFVFFSAETLTEAQYRQSPSRASLPSSD
                     FNAYLQVKEDGRVACFTGKVELGQGIITSLAQMLAEELDLPLDSVDMVMGDTDLCPYD
                     AGTFGSRTTKYFGPQLREAAAQARAILLELAATRLNVPQAFLTISHGIIHDSRNPEKR
                     VAYADLVKGTRIERKAEVKAEPKPPSSFKVSGRPAGRTDGLPKVTGQARFAGDIRLPD
                     MLYARILRPPAHGATLKKVDTSAAEKIQGLKIIRNGNLLAVLHPLPDQAEMGLALIKA
                     QFATPSPAVNENSIFSHLLKVAPEGSVISQEGSLEKGEKSSSRKFDESYFQHYVAHAP
                     METHTAMAHVEGKKATVWASTQTPFGVKEAVAQALSIPSEGVRVITPFVGGGFGGKTW
                     NQQAVEAALLSHRAGKPVQVSWTREEEFFYDAFMPAAVVKIRSGLDSQGRITFWDYRV
                     YFASERGAATFYDIPSHRAAVHGSLWGGAGAHPFNTGAWRAPGTNTNTFARESHMDLM
                     ASAAKADPLAFRLAHLKNSRMRRVLEEAARSFGWRDFPRKPGRGKGIACVDYLGTYVA
                     VAAEVEVDPQKGDVQVRRLVCAQDMGQVINPEGARMQIEGCLMMGLGYALSEEVHFRG
                     GEIQDKNFDTYRIPRFSWMPKIETVLIENQDLPPQGGGEPAITSMGAVLANAVYDATR
                     ARLFELPMTPERIQKAMARAPHKKA"
     gene            complement(3620..4096)
                     /locus_tag="VEL68_06185"
     CDS             complement(3620..4096)
                     /locus_tag="VEL68_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ35587.1"
                     /translation="MERIEFQLNGQTMKLEVEGDRKLLWVLRSDLNLPGTKYACGKGL
                     CGACTVLIDEEAQRSCQVPMREIAGKKVVTIEGLAKGERLHPLQKAFLEQNAFQCGFC
                     TPGMILSAYSLLKRNSYPSEAEVLQAMDGNLCRCGAHPRIVKAIQTAAREMKEKKG"
     gene            4416..>5147
                     /locus_tag="VEL68_06190"
     CDS             4416..>5147
                     /locus_tag="VEL68_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXZ35588.1"
                     /translation="MGNQTQGAQVFGIGQCSLDYIGKIEAYPPPDRKCEFVDMVIQSG
                     GPVATALVALARWGISCAFAGVLGDDLFGRMIRESLEGEGIDTAGVMIRRGKGSQFAF
                     IVAEPGLGRRTIFWRRPTGPPLSPEEIDLRRVEQARILHTDGRFVEASLACCKTARKA
                     GVQVVTDADSFHEGLVELARWSDFFISSENFASAFLQKEDPREACQRIAELGPKVAGV
                     TLGAKGYVALAEGRWIEKPAYPVEAV"
BASE COUNT         1100 a         1431 c         1494 g         1122 t
ORIGIN      
        1 aagacaaaga tttttctctc gcccgctccg ctggaggccg cagagaacgc agagaagaca
       61 aagcagaaaa caggagtcag gaatcaggaa cctgaatcct gagacctggg acccaaatcc
      121 tttttgacac tgtggtccct gagggctctg cgagagaaat tttggcgatt cacgagtcgc
      181 cccggagaac accctcttca gggcatccct ttggtttccg agtaaaaggc ctgcccccac
      241 ttcagggcct cgagatagag ggagaagggg gttgtttctt cccccggggg aatcatcggt
      301 ttttccagat gcccccaggc ggctttttcg taggagaggg tgtattgata aatatcccgc
      361 aagcggttgg gctcgcccag aagggccagc ttgatgggtt catcgatggg gatctcggcc
      421 aggatggcgg aaagcggccg gtccaggaaa gcgtcgatca gggaaaaaag acccatcagg
      481 aaagaatcct ctttccggtc cgaaaggtca aaataagggg ccagcatctc acagaacctg
      541 gctcgaacga tggtcataat caaaagctcc tccgccttgt ccgcccccat gcttgccagg
      601 gccacaaaag tgatccagtt tttgatggtt ctctctccca ggaggatcat ggcctgcttc
      661 accgacgtga ttttgctccg gaggccaaag aaggctgaat tgatgtagcg cagaagctta
      721 taggtgaagg caacttcttg tttaatgatc tcttccagcc tattgaggtc gatctcggcc
      781 tggtggatct ccttcagcag ctgaaaataa tgggccttga atccggggat gtcctttccc
      841 acgatgatcg cgggcttgct gaaaaagtaa ccctggaaaa aggagtacct actctcaatt
      901 ccctcctcga acatctggtg cgtctccact ttttcggcca cgaggcggac cttgtaggga
      961 gagaccttct cgaagagcgc cttccgggct agctccgggg tggacaagaa atccactttt
     1021 acgaaatcgg tgaactccag caggggctcg tagcggtctt catacacaaa gtcgtccacg
     1081 gcgatcagat acccggcccg tttcagtttt tgacaggcct gcaggacttc cggatcgggt
     1141 tctaccgttt ccagcagttc caccacaacc tggtccttgg ggaggaagaa catatattct
     1201 tttaataaga tgtcccgggg aacgttgatg aaggctctcg ttccgccggt gaggtcatcc
     1261 aggttaaaga gaaaaaagct gtccaccatt actttggaag tagcctggct ggggtccgag
     1321 tgtttaaaga cgttttccag accggagcgg aaaaagagct catatccata caccctgcgt
     1381 tgggcgtcga aaatcggctg gcgggcgatg aaggtttcca taggggtctg tcctttttaa
     1441 attgctttcc ttcagtgggc ggttcctgaa tcgcccccaa tccctggggt ggaccgtcgc
     1501 taagcctttt tatggggagc ccgggccatg gctttctgaa tcctctcagg agtcatgggc
     1561 agttcaaaca gcctggcccg agtcgcatca tacaccgcgt tggcgaggac ggcacccatg
     1621 gaggtaatgg ccggttctcc ccctccctgg ggaggcaggt cctggttctc gatgagcacg
     1681 gtctcgattt tgggcatcca agagaaacgt ggaatccgat aggtatcgaa attcttatct
     1741 tggatttccc ctccccgaaa atggacctct tccgagaggg cataccccag ccccatcatc
     1801 aggcagcctt cgatttgcat ccgcgcccct tcgggattga ttacctggcc catgtcctga
     1861 gcgcatacga gtctgcggac ctgaacgtct cctttttggg ggtctacttc gacttccgcc
     1921 gctacggcca catacgtacc caggtaatcg acgcaggcga ttccttttcc ccggcccggc
     1981 ttacgcggaa agtcacgcca tccaaaggaa cgtgcggctt cctccagcac cctgcgcatg
     2041 cgcgagtttt tcaggtgggc cagccgaaat gccagagggt ctgccttggc cgcagaggcc
     2101 atgaggtcca tatgggattc ccgggcaaag gtattggtat tcgtccccgg agcccgccag
     2161 gcgccggtgt tgaaaggatg agccccggcc cctccccaca agctgccgtg gactgctgcc
     2221 ctatgagagg ggatatcata gaaggtggcc gcgcccctct cactggcaaa ataaactcga
     2281 tagtcccaga aagtgattct gccctgggaa tcgagcccgg accgaatctt taccacggca
     2341 gcaggcataa aggcatcgta aaagaattcc tcctcccggg tccaggacac ttggacgggt
     2401 ttcccggcgc gatgagaaag gagggccgcc tcgaccgcct gctggttcca ggtttttcct
     2461 ccgaaccctc caccgacaaa aggggtaata accctaacac cttcagaggg aatcgagaga
     2521 gcctgggcca cagcctcctt gacgccaaaa ggggtttggg tggaggccca tacggtggcc
     2581 ttcttccctt ccacgtgggc catggcagtg tgggtctcca tgggggcgtg ggctacataa
     2641 tgctggaaat aactctcgtc gaatttcctg gaggaactct tttctccctt ttccagggat
     2701 ccttcctgag agattacact cccctcaggg gcaaccttca gcagatggga aaagatggaa
     2761 ttctcattca ccgcaggaga aggggtggca aactgggctt tgatgagggc caggcccatc
     2821 tccgcctggt ccggaagggg atggaggacg gccaggagat ttccatttcg gatgatcttg
     2881 agtccttgaa tcttttccgc ggccgaagta tccaccttct tgagggttgc cccatgagcc
     2941 ggaggacgca gaatgcgggc atagagcatg tccggaaggc gaatgtcccc ggcaaaccgg
     3001 gcctggccgg ttaccttggg gagtccatcg gtccgtccag cgggcctgcc tgaaacctta
     3061 aaagaggaag ggggtttggg ttcggccttg acctccgcct ttctctcaat ccgggtccct
     3121 ttcaccaagt cggcataggc gactctcttc tccggatttc ttgaatcgtg gattatgcca
     3181 tggctgatcg ttaggaaggc ctgagggaca ttcaggcggg tagccgccag ctccaacagg
     3241 atggcccggg cctgcgccgc agcttcccgc aactgagggc cgaaatattt tgtggtgcgg
     3301 gagccgaagg tccccgcatc ataagggcaa aggtcggtat cccccatgac catgtcgacg
     3361 gaatccaggg gaagatcaag ctcctcggcc agcatctggg ccaatgaagt aatgattccc
     3421 tgtcccaact ccacttttcc ggtgaagcag gccacccggc cgtcttcctt gacctggaga
     3481 taagcgttga aatcggagga ggggagggag gcccgtgatg ggctctgtcg gtattgggct
     3541 tcggtcaggg tttcggcgga gaagaagacg aagagccctc cgcccatggc cttcaagaac
     3601 tggcgccggt ccagagagct catccctttt tctccttcat ctcccgggcg gcagtctgga
     3661 tggccttgac gattcgcgga tgggcaccgc atcggcagag gttgccatcc atggcctgaa
     3721 ggacctccgc ctcggaaggg tacgaattcc tttttaatag gctgtaggca ctcaggatca
     3781 ttcccggggt gcagaaaccg cactgaaaag cgttttgttc gaggaatgct ttctgcaggg
     3841 gatgaagcct ttctcccttg gccaggcctt cgatggtaac cactttcttc ccggcaatct
     3901 cccgcatcgg gacctggcag gaccgctggg cttcctcatc gatgagaacc gtacacgccc
     3961 cgcagaggcc cttcccgcag gcgtatttgg tccccgggag gtttaggtcc gagcgcaaaa
     4021 cccagaggag ctttcggtcc ccctccacct ccagcttcat agtctgccca ttcagctgga
     4081 attcgatccg ctccatcctt tcctccccca tcagggtttt catcctttta tccagtttat
     4141 agcacccccg gagggccggt caatcctttt gaaaaccttg cccacgccca gtttttgaga
     4201 aaaaagtcct tcgcccaatg gagtgcgcga agcgggaggg ggaacaccaa cgagcacgag
     4261 aaatcggggg tgggcaagcc ttaaaaccca tccgaaggag ctattgacca acccccccct
     4321 ccctcgtctt gcaagccatt gggcgaatgg agtaccctga atcgtacgag agaagaggat
     4381 tttttctttt ggcgggaagg gtcggagaaa aagaaatggg aaatcaaact cagggggccc
     4441 aagtcttcgg aatcggccag tgctccttgg attacatcgg gaagatcgag gcctatccgc
     4501 ctccagatcg gaagtgtgaa ttcgtcgata tggtcatcca gagcgggggg ccggtagcca
     4561 cggctctggt ggccctggca cgatggggaa tttcctgcgc ctttgccggg gttctcgggg
     4621 acgatctctt tggccggatg atcagggaat ccctggaggg cgaaggaatc gacaccgccg
     4681 gagtcatgat tcgaagggga aaaggctccc agtttgcctt tatcgtggcc gaaccgggac
     4741 tgggaaggag aaccattttt tggcggaggc ccacgggacc tcctttgtcc cctgaagaaa
     4801 tcgatcttcg tcgagtggag caagccagaa tcctgcacac cgatgggcgt ttcgtggaag
     4861 ccagcctggc atgctgcaaa actgcgagga aggcgggggt tcaggtggta acagacgcgg
     4921 attccttcca cgaaggattg gtggagcttg cccggtggag tgattttttt atctcctcgg
     4981 agaattttgc ctctgcgttc ctgcagaaag aggacccccg cgaggcctgc cagcgcatcg
     5041 ccgagctggg gcccaaggtt gccggggtga ccctgggagc gaagggctat gtagccctgg
     5101 ccgaaggccg ctggattgag aaacctgcct acccggtaga ggcggtg
//
LOCUS       DASPRR010000157         5480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1166231, whole genome shotgun sequence.
ACCESSION   DASPRR010000157 DASPRR010000000
VERSION     DASPRR010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166231"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VEL68_06195"
     CDS             complement(<1..795)
                     /locus_tag="VEL68_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ35589.1"
                     /translation="MNSDQLAIAKAVESRQEKFWKMSDAIWSYAELGLEEYRSAKLLA
                     DGMEAAGFKVERGVAGMPTAFIATWSYGSGKPVIGFLGEYDALPMLSQKAGSPKKDPL
                     VPGAPGHGCSHNTMFVTQALSAVTLKEFMEKKGLNGTLKIYGSPAEEILTSRPFMVKA
                     GLFEGVDVVLDNHGDSLFQVAYGMEGTAMVSFIVTFKGKTAHAGAMPWMGRSATDAVE
                     LMHAGTERMREHLPTTQRTHWVTLEGGEAPNVVPDRASTWYFIRDLD"
     gene            complement(948..2543)
                     /gene="serA"
                     /locus_tag="VEL68_06200"
     CDS             complement(948..2543)
                     /gene="serA"
                     /locus_tag="VEL68_06200"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXZ35590.1"
                     /translation="MFRILITDDIGHAGLTLLEAAADVKHDLVKLPAPEKLIEMIGDY
                     DGVITRSGTPLTAPVFEAARRLKVAGRAGVGLDNIDIEAATLHGVLVMNTPEANTLAA
                     TELTLGLMLALCRNIPRANASIKKGEWTRSKFLGVQLHGRTLGVIGLGRIGSRVATRC
                     QAFGMKVMAYDPYIAEEAAERIHVRLVGSLEELLREADLITVHTPLTEETRGMIGAKE
                     IALMKEGVRLINCARGGIMDEKALLDALSAKKVAGVGLDVYGKEPPKGELIQQLLSRE
                     EVVATPHIGANTWEAQRDVAVQVVQQVIEALRGENFRNVVNLPFPEGVDYRSLAPYME
                     LAEKMGSLHMQMIRGRISRVEVEFRGEDVEANGKPLTVALLKGILSPILSEDVNYVNA
                     PHLADQRGIQVSQTRHPAAQDYSNVILCRAFSTKETRLIGGALFLHKIPRIVLLDDYR
                     IDALPSGPALILSNRDIPGVIGKVGTLLGEKGINIAEWQMGRNERGGMAVSFINIDTP
                     ASDSVLQELRALSPILEVRQVKL"
     gene            2786..3328
                     /locus_tag="VEL68_06205"
     CDS             2786..3328
                     /locus_tag="VEL68_06205"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HXZ35591.1"
                     /translation="MEEGIKTGESFVPKESGLITLLENRGLILAFRLILAGIFLLSSF
                     GKLVDIERYSVDAVYDFGILPMVLARPFGLVMPFIELLCGLGLLFGVLTRLSALGVAL
                     MSLAFFMAKAIVLAEGRTIECGCFGAVVDTLASMTIFMDIPMFLLSLVVMRAPSSARH
                     WMAVGKRLSQRWKEKLNRVW"
     gene            3383..5143
                     /locus_tag="VEL68_06210"
     CDS             3383..5143
                     /locus_tag="VEL68_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3785..3787,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXZ35592.1"
                     /translation="MPKKNVLRVFWAAAILVGLLGLPSLAAAKEQVQEWEVINPSGTI
                     SIQPIKPAPRLASLEGKTIVLRWNGKHNGNNYLDRVAELLAEKMPSAKVIKVYDLDKS
                     TVKISGSNAESARIAKVIKDLKADIVIGSQADUGSCTSWLVVDQSEVEKLGIPTVTVA
                     TTAFLSLARSTMESIGLADMAFVEVPHPMGMVSLADIRAKADQSFAEILQKAVQWKAS
                     RTEIPGLGKPPYPAERIKFRGTYESLNKMFYEKRWSLGLPVIPPAPEAVAEMLKGTSR
                     QPSEVVWVVPPRMGQLTVELVATLGVMAGCKPEHMPLLLAMIKAISHPHFDWRGSSTT
                     TAPTTPVIVINGPILDELGIGYSTGALGGEQPVNIALGYFVNLVGDIVGGSMPPDPDK
                     STLGSREDLVAIVIGENEKQNPWKQSYAVDLGFKPTDNVVTAYSAYMGTNNTDHTSVK
                     GTQLLNTLSLGAAAVACGITSCFTQYEKPYAIDNRVKFVFLFLAPEHADTIYKDFPNK
                     RAVQEYVVKKAVAPYWSYAPEICTVPKEFGPYDENTMIPRFTKPEQVHIVVTGGPGKQ
                     SQVWPPFTTVFKPVSVLVEK"
BASE COUNT         1121 a         1573 c         1605 g         1181 t
ORIGIN      
        1 atccaggtcg cggatgaagt accaggtaga ggcccggtct gggaccacgt tgggcgcctc
       61 tcccccttcc agggtgaccc agtgggtcct ctgggtagtg ggcaaatgtt cgcgcatgcg
      121 ttcggttccg gcgtgcatga gctcgacggc atcagtggcg ctccttccca tccagggcat
      181 ggcaccggca tgggcggtct tccccttgaa ggtcacgatg aaggaaacca tggccgtccc
      241 ttccatccca taggctacct ggaaaagact gtccccgtgg ttgtccagga cgacgtcgac
      301 tccttcaaac agcccggcct tcaccatgaa agggcggctg gtcagaatct cttccgcagg
      361 agagccgtag attttgagag tcccgttcaa cccttttttc tccatgaatt ccttgagggt
      421 gacggcgctc aaggcttgag tgacgaacat ggtgttgtgg ctgcagccgt ggcccggggc
      481 gccggggacc agggggtctt ttttagggga cccggccttc tgggaaagca tgggcagggc
      541 atcgtattcc cccagaaaac cgatgacggg cttgccgctg ccgtaactcc aggtcgctat
      601 aaaggccgtg ggcatcccgg ccaccccccg ctccactttg aatcccgccg cctccatccc
      661 atcggccagc agcttggccg accggtattc ttccagtccc aactccgcat agctccagat
      721 ggcatccgac attttccaga acttttcctg cctcgattca acggccttgg cgatggccag
      781 ttggtctgaa ttcatcccat cctcccctgg ttttaataaa acgcatagag cctggcgcat
      841 ggctcccatt cggagatatc cttgagatca tacctcccag cacgggttgt aagggcgata
      901 catgtcggag actcgactct acgagaatcg cccttgcggc gcgggggtca gagctttacc
      961 tgccggactt ccagaatcgg ggaaagggcg cggagttcct gcaggactga gtcgcttgcc
     1021 ggcgtgtcga tgttgatgaa agagacggcc atccccccgc gttcgttccg ccccatctgc
     1081 cattccgcga tgttgatccc cttctctccc aggagcgttc ccaccttgcc gatcacccct
     1141 ggaatgtccc gattggagag aatcagggcc ggccccgagg gaagggcgtc gatccggtag
     1201 tcgtccagga ggacgatccg gggaatcttg tgaagaaaga gagccccgcc gatcagacgg
     1261 gtttctttgg tggagaacgc tcggcagagg atcacgttgg aataatcctg agctgcgggg
     1321 tgacgggtct gggaaacttg gatcccccgc tggtcggcca gatgaggggc attgacgtaa
     1381 ttcacgtctt cgctcaggat gggggagagg attcccttca gcagggcgac agtcagaggt
     1441 tttccgttgg cttccacgtc ttcgccgcgg aactccactt ccacccggct gatccggccg
     1501 cgaatcatct gcatgtggag cgatcccatt ttttccgcca gttccatgta gggggccagg
     1561 gaccggtagt ccacgccttc ggggaagggc aggttgacca cattgcggaa attctctccc
     1621 cgcagggctt cgatcacctg ctgcacgacc tgcacggcca cgtctcgctg agcctcccag
     1681 gtgttggccc cgatgtgggg agtggcgacc acttcctccc gggaaagaag ctgttggatc
     1741 agttcccctt tgggaggctc cttgccatag acatccaggc ccacaccggc caccttcttc
     1801 gctgataggg cgtcgaggag ggctttttcg tccatgattc ctcctcgggc gcagttaatc
     1861 agccggactc cttccttcat gagggcgatc tcttttgcgc cgatcatgcc gcgggtctcc
     1921 tcggtaagag gggtgtggac ggtgatgagg tcggcctcgc ggaggagttc ttccaggctg
     1981 cctactaggc ggacgtgaat ccgctcagcc gcttcttcgg cgatgtaagg atcgtaggcc
     2041 atcaccttca ttccaaaggc ctggcaccgc gtggccaccc gcgaaccgat ccggcccaat
     2101 ccaatgaccc ccagggttct gccgtgcaat tgcaccccca ggaatttgct ccgggtccat
     2161 tcccccttct tgatcgaggc gttcgcccgg ggaatattgc ggcacagggc cagcatcagg
     2221 ccgagggtca gctcggtagc cgccaaggtg ttggcctcgg gggtgttcat caccagaacc
     2281 ccgtggaggg tggcggcttc gatatcgatg ttgtccagcc ccaccccggc tcggccagcc
     2341 actttcagcc gcctagccgc ttcgaagacc ggagcggtca ggggagttcc cgagcgggtt
     2401 atcaccccgt cgtaatcccc gatcatttcg atcagttttt ccggggcagg aagtttcacc
     2461 aggtcgtgct ttacgtccgc ggcagcttcg aggagagtca gcccggcgtg ccctatgtca
     2521 tcggtaatca gaattcgaaa catcgcggaa tccctctggt cattttttct ctcctttaga
     2581 atactcatcc agaaaaaata tgcaaggttt gttccccaat cgcagtcctt ttcggaggag
     2641 atgaattctc aggagcccca agaagggtat tttgggttga aatttccccc gtcgttcagt
     2701 atgttgacct tgggggcaga ggtaccagaa ttggtgttat ttaaagggct ggaacgcccc
     2761 ttcggtgcac aatgaagagg tgagaatgga ggaaggaata aaaacaggcg aatctttcgt
     2821 gccgaaggag agtgggttaa taacccttct ggagaaccgg ggtttaattc ttgcctttcg
     2881 gctgatcctg gcgggcatct ttctcctttc ttccttcgga aagctggtcg atatcgaacg
     2941 ttactcggtg gatgcggtgt acgacttcgg catcctgccc atggtgctgg cccgtccctt
     3001 cgggctggtt atgcccttca tcgagctcct ctgcggactg gggctcctct tcggggtcct
     3061 gacccgcctg tccgccctgg gcgtggccct catgagcctg gcttttttca tggccaaagc
     3121 catagtgctt gccgagggga gaacgatcga atgcggatgt ttcggtgcgg tcgtagacac
     3181 tctggcgtca atgaccattt tcatggatat ccccatgttc cttctttccc tggtcgtgat
     3241 gagggccccg tcctccgccc gccattggat ggcggtcggg aaacgtttgt ctcaaaggtg
     3301 gaaggagaag ctaaatcggg tatggtgagg actcccgttg gaagaaagtg atcctaagcc
     3361 caaaagaaag gagagattaa acatgccgaa aaagaatgtg ctcagagtct tctgggcggc
     3421 agccattttg gtcggccttc tgggtttgcc gtcgctggcc gcggccaaag agcaggtcca
     3481 ggaatgggaa gtcatcaatc cttcgggcac gatcagcatc cagccgatca aaccggctcc
     3541 gcggttggcc agcctggagg gcaaaaccat cgttctccgc tggaacggga agcacaacgg
     3601 gaacaattac ctggatcgtg tggctgagct cctggccgag aaaatgccca gcgcgaaggt
     3661 gatcaaagtt tacgacctcg acaagtcgac agtcaagatc agcggctcca atgccgaatc
     3721 cgcccgcatc gccaaggtca tcaaggatct caaagccgac attgtgatcg gctctcaagc
     3781 ggactgaggg tcctgcacct catggctggt agttgaccag tccgaagtgg agaaactggg
     3841 aattccgacg gtgactgtgg ccaccactgc tttcctttcc ctggccagaa gcaccatgga
     3901 gagcataggc ctcgcagaca tggcgtttgt ggaagtaccc catcccatgg gcatggtttc
     3961 gctggccgat attagggcca aggccgatca gtcctttgcg gagattctgc agaaggctgt
     4021 ccaatggaag gcctcgcgaa ccgaaatccc gggcctggga aagccgccct acccggcgga
     4081 gagaatcaaa ttcaggggaa cctatgagtc cttaaacaag atgttctacg aaaaacggtg
     4141 gtctttggga ttgccggtca tcccgcccgc gccggaggca gtggccgaga tgctgaaagg
     4201 tacgagccgg caacccagtg aagtggtctg ggtggttccg ccccgtatgg ggcagttgac
     4261 cgtggaactg gtggctaccc tgggggtcat ggcggggtgc aagccggagc atatgcctct
     4321 cctactggcg atgataaaag ccatcagcca tccccatttt gattggcggg gcagctccac
     4381 aaccacggct cccacaactc cggtgatcgt cataaacggg ccgatcctcg atgaattggg
     4441 catcggctat tccaccggtg ccctgggcgg agagcagccc gtgaacatcg ccctggggta
     4501 tttcgtgaac ctggtcgggg acatcgtggg cgggtctatg ccccccgacc cagataaaag
     4561 caccctaggg agccgggagg acctggtggc cattgtcatc ggcgagaacg aaaaacaaaa
     4621 tccctggaaa cagtcttatg ccgtagacct agggttcaag ccgacggaca acgtggtaac
     4681 cgcctattcg gcctacatgg ggaccaacaa caccgaccat accagcgtga aaggtaccca
     4741 gctgctgaat accctgtccc tgggggcggc agcggttgcc tgtggaatca ccagctgctt
     4801 tacccagtac gaaaagccct acgccattga caaccgggtg aaattcgtct tcctcttcct
     4861 ggcgccggag cacgctgaca ccatttataa ggacttccca aacaagcggg ccgtccagga
     4921 atatgtagtg aaaaaggcgg tggcccctta ttggagctat gcccctgaaa tctgcactgt
     4981 gcctaaggag ttcgggccct acgacgaaaa cacgatgatt ccccggttca ccaagcccga
     5041 gcaggtccat atcgtggtga ccggcgggcc cggaaaacag tcgcaggtct ggcctccatt
     5101 caccacggtg ttcaaaccgg tgtcggtttt ggtggaaaaa tagaggaacc atcccggtca
     5161 ttcaccctct ggcgtggaca aaaggccggt gagtcggcgg gaaaaagact gaagagaagg
     5221 gagagcctct tttctgggag aggttctccc ggtccccata ctgtaacatt cctcctccag
     5281 attctgattc ccctgatcgg agagaaggac ccggaagatg cgaaggtgta ccttcctccc
     5341 attcttgatt ctatgcgtta ctgatcagtc cataattgtg tggacgctga ggggaaagcc
     5401 gttcgtgctg agcttgtcga agcatgaacg gcattacgac gcacccttcg acaagctcag
     5461 ggtgaacggt gacaggcaaa
//
LOCUS       DASPRR010000158         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_527561, whole genome shotgun sequence.
ACCESSION   DASPRR010000158 DASPRR010000000
VERSION     DASPRR010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527561"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VEL68_06215"
     CDS             <1..674
                     /locus_tag="VEL68_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35593.1"
                     /translation="GDIQALREVSLSVSTGEIVSLVGGNGAGKTTALKTISGLLRPAS
                     GSILFQEAEIHRMPAHRIVEKGLSQVPEGRRVFPRMSVLENLHMGSHVREARKRREET
                     LGWVFRLFPVLKERREQFGGTLSGGEQQMLAIARGLMCRPKLLLLDEFSLGLAPLIVR
                     EIFAIIQEINQQGTTVLLVEQNVQRSLAISQRGYVLENGAIVLQGGGKELLKNEYMMK
                     AFLGR"
     gene            867..2048
                     /locus_tag="VEL68_06220"
     CDS             867..2048
                     /locus_tag="VEL68_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35594.1"
                     /translation="MNFDLTEEQAAMERMAKNFAEKEIVPNLKREEFNRELVRRMGEL
                     GLFGSAFPEGMGGSASGFLAHSVICEAISTYDSGLRALFNLQAMTVPYTMMEWGSDSV
                     RKKYVKPLVSGEKLGCTCFSEPNAGSDIAAIETKVVDKGDHFLINGSKTWISNGTVAE
                     YAVVYCTHDRSLKHNGLCAIVVETKQPGWQPHEIPKLGDKASPIAEIGLEDVVAPKEN
                     LLGEWGGGFKVAMTALDRGRISVGSGALGICQACLNASVKYANERIQFGRPIRDYQMV
                     KHLIADMVTYTETARLMVRRGAWLNDQGRPFTREIAVAKYFAGEAAVRAAGWAMEIHG
                     GMGYSLELPVEKYYRDSKLYQVGEGTANVMRLLIADDALGLKKANRPRLKVPSDFRDL
                     S"
     gene            2227..3003
                     /locus_tag="VEL68_06225"
     CDS             2227..3003
                     /locus_tag="VEL68_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ35595.1"
                     /translation="METLVLEKKGHFCWLKLNRPDALNALNTQMAKEVLEACQELALD
                     REVWVVGLTTTSEKSFGVGADLKERNQMSVGDLARQRVLFVRAMNALRAIPQPVIAGV
                     KGFALGGGFEMALAADIIVAGENAQFGLPEVRVGLIPGNGGTQNLPRVIGKMRAKEMI
                     FTGKRISAQEAMAMGIVSKVVPVAQVEETVFKVAEEICANSPIAVRQAKRAVDLGMEV
                     DLNTGFQIEIEAYNVNLVTEDRQEGIRAFNEKRPPKWQNL"
     gene            3240..4256
                     /locus_tag="VEL68_06230"
     CDS             3240..4256
                     /locus_tag="VEL68_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HXZ35596.1"
                     /translation="MAILNYPKKVVLADLTVREGFQHEEKTIPLRAKLWVVEQLVLAG
                     FKRLEVTNLGTPVGMPQFADADQLLQGIHNSKVLKDRLQDVQLSAVTIRERAADRAIE
                     ARRQGWGPDRIVFMVSTSESHHRRNSGLSLEEYWKMVEKYVSKAHENGIKVCGTVSTI
                     WGCPIEGPTDLKKAVEFARRYLDMGADEIEHADHDGSATPDKVYEYFSMVLDKIPNPK
                     LHITHFHVTRGWGLANVLAALQAGITHFESTLGGLGGQPANFIDGYPVSGTGRYYYTD
                     PNIVGLVSTEDMVVMMDEMGIETGIDVNRLLDIGRMVEKIVGRRLRSECIHTGPIPKG
                     HTGR"
     gene            4445..4855
                     /locus_tag="VEL68_06235"
     CDS             4445..4855
                     /locus_tag="VEL68_06235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXZ35597.1"
                     /translation="MEIADQDKYGFCFACGKKNPIGLKLDFRFEEGKAKAEFTPGPYH
                     QSWNGIFHGGLLALCLDEAFGYVLYFQGIKGMTAKMEMRIRKTVQTGQKILLTGEVKR
                     KTRKIIETYAQAEFEDGTLAAEATATMYVTDRDF"
     gene            4902..5036
                     /locus_tag="VEL68_06240"
     CDS             4902..5036
                     /locus_tag="VEL68_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35598.1"
                     /translation="MRFASHRQPAEEANLTRSGTNRQKRQGSKAANEYLEGVQTPCIL
                     "
BASE COUNT         1193 a         1381 c         1513 g         1037 t
ORIGIN      
        1 acggcgacat ccaggccctg cgggaagttt ccctctcggt ttccaccgga gaaatcgtct
       61 ccctggtggg aggaaacggc gccggcaaga ccactgcgct caaaaccatc tccgggctgc
      121 tccggccggc ttccgggtcc attctcttcc aggaagccga aatccatcgg atgcccgccc
      181 atcgaattgt ggaaaaaggc ctttcccagg tgcccgaggg acggcgggtc tttccccgca
      241 tgtcggtcct ggaaaacctc cacatggggt cccacgtgag ggaggcccgc aagaggaggg
      301 aggaaaccct gggctgggtc ttccgactgt ttccggtctt gaaggagagg agggaacagt
      361 tcgggggcac tttgagcgga ggagagcagc agatgctggc catcgcccgg ggattgatgt
      421 gccgccctaa gcttctgctc ctggatgagt tctccctggg gctcgctccc ctcatcgtgc
      481 gggagatctt cgccatcatc caggagatca accagcaggg caccacggtc cttctggttg
      541 agcagaacgt ccagcggtcc ctggccatct cccaacgagg gtatgtgttg gaaaacggag
      601 ccattgttct tcagggagga gggaaggaac tgctgaagaa cgaatacatg atgaaggcct
      661 ttttgggaag gtgatccctt attgcggatt tcagattgca aagaatatcc catgtcaggt
      721 tctgcctgaa accacccccc cgtactctgt ttccttcttt ctttccttga cctttcccgg
      781 caaaaatatt aacctctggt cgaaagggga agcgaaagga ccggggtcct accattccgc
      841 aatttatccc cttcgaggga gaggcgatga acttcgatct gaccgaagag caggctgcca
      901 tggaacgaat ggccaagaat ttcgccgaaa aggaaattgt tcccaatttg aaaagggagg
      961 aattcaaccg cgaactggtc aggagaatgg gagaactggg tcttttcggc agcgcctttc
     1021 ccgaggggat gggggggtcc gcttcgggat ttctggccca ttcggtgatc tgcgaggcca
     1081 tctccactta tgattccgga ttgcgtgccc tcttcaacct ccaggccatg accgttccct
     1141 acaccatgat ggaatggggc agtgattcgg ttcggaagaa gtacgttaaa cccctggtct
     1201 ccggcgagaa gctgggctgc acctgtttct ccgagcccaa cgccggctcg gatatcgccg
     1261 ccatcgaaac caaggtggtg gacaaggggg accacttcct gatcaacggg tccaagacct
     1321 ggatctccaa cggcaccgtg gccgagtacg cggtggtcta ttgcacccat gaccgttccc
     1381 tcaagcacaa cgggctctgc gccatcgtcg tggaaacgaa acagcccggc tggcagcccc
     1441 acgagattcc caaactgggg gacaaagctt cccccatagc ggaaatcggg ctggaagacg
     1501 tggtcgcccc taaggaaaac cttctggggg aatggggggg cggattcaag gtggccatga
     1561 ccgccctgga tcggggacgg atcagcgtgg gaagcggcgc cctgggcatc tgccaagcct
     1621 gcctgaacgc ttcggtaaag tacgccaacg aacgcatcca gttcgggcgc cccatccggg
     1681 actaccagat ggtcaagcac cttatcgccg acatggtgac ctacaccgaa accgcccgtc
     1741 tcatggtccg gcgcggagcc tggctgaacg atcagggaag gcctttcacc cgggagatcg
     1801 ccgtagccaa atactttgcc ggcgaggctg cagtgcgggc cgccggctgg gccatggaga
     1861 tccacggcgg catgggctat tccctggagc ttcccgtaga aaagtactac cgggattcca
     1921 agctctacca ggtgggagaa ggaaccgcca acgtcatgcg gttgctcatc gccgacgatg
     1981 ccctgggtct gaagaaagcc aaccgccccc gcctgaaggt tcccagcgat tttcgtgacc
     2041 taagttaatg tgatgaggtc gtaaaaagtg gtttttaccc cgttttcgtc attcccgcgg
     2101 aagcgcgaat ccagttaaat caaacatttc tggactcccg cctgcgcggg agtgacagcc
     2161 tttctgactt tttacgaatt catctaaatt gtccagcgcg tgaaaaatgt gttttaagga
     2221 gaagagatgg aaactcttgt cttggagaaa aaaggacatt tctgctggct caagctgaac
     2281 cggccggacg cactgaatgc actgaacacc caaatggcca aagaggtcct ggaggcctgc
     2341 caggaattgg cccttgatcg ggaggtctgg gtcgtgggac tgacgactac ctccgaaaag
     2401 tcattcgggg tgggagccga tttgaaggag cgcaaccaga tgagtgtagg agacctggcc
     2461 cggcagcgag tcttgttcgt ccgggcgatg aatgccctga gggcgattcc ccagccggtc
     2521 atcgccgggg tcaaaggctt tgccctgggc ggaggttttg aaatggccct tgccgcggat
     2581 attatcgttg cgggggaaaa cgcccagttc ggcctgccgg aagtgcgggt gggcttgatt
     2641 cccggcaacg gggggacgca gaacctcccc cgggtcatcg gaaagatgag ggccaaggag
     2701 atgatcttta ccggaaagag gatcagcgcc caggaggcaa tggccatggg gatcgtgagt
     2761 aaagtggtcc cggtcgccca ggtggaggag acggtcttca aggtggccga agaaatctgc
     2821 gccaattccc ctattgcggt taggcaggcc aagcgggccg tcgatttggg aatggaggtc
     2881 gacctgaata ccggctttca gattgaaatt gaagcctaca atgtgaacct ggtcactgaa
     2941 gatcggcagg aaggaatccg ggccttcaac gaaaaaagac cgccgaagtg gcaaaatctc
     3001 taggtgcata gagcatggcg cagacagaaa atgccaaaga ttccatgccc ctttctttat
     3061 aatattaaca tgcagaatcg ctaaaattta ttaagggctt ttccgaaatg taggggcggc
     3121 cccctgtggc cgcccacaag atgggcaacc acggggggtt gcccctacca ttcggttgct
     3181 gctctgacgc acagtgctct acgcggttct gaattttccc aaagggagaa gaaaccggca
     3241 tggcgatact gaactacccc aagaaggtgg tcctcgcaga cctaaccgtt cgcgaaggat
     3301 ttcagcatga ggaaaagacc attcccctca gggccaagct ctgggtggtg gagcagctcg
     3361 tcctggcggg tttcaagcgg ctggaagtga ccaacctggg cactcccgtc ggcatgccgc
     3421 agttcgccga cgcggatcag ttgctccagg ggatccacaa tagcaaagtc ctcaaggaca
     3481 gactccagga tgtgcaattg agcgctgtga ccatccggga gagagccgcc gaccgggcta
     3541 tcgaggcccg ccggcaaggc tggggcccgg accgaattgt cttcatggtg tccaccagtg
     3601 aatcccatca ccggcgcaac tccggccttt ccctggaaga atattggaag atggttgaaa
     3661 aatacgtctc caaagcccat gagaatggga tcaaggtttg tggtaccgtt tccacgatct
     3721 ggggatgtcc catcgaaggc ccgacggacc tgaaaaaagc ggtggagttc gccagacggt
     3781 atctcgacat gggggccgac gaaatcgagc atgccgacca tgacggttcc gcaacgcccg
     3841 acaaggtgta tgaatacttc tccatggtcc tggacaaaat ccctaacccg aaactgcaca
     3901 tcacccattt tcacgtaacc cgcggctggg gcctggctaa tgtcctggcg gctctgcagg
     3961 ccggcatcac ccacttcgaa tccaccctgg gcggcctggg aggacagccg gccaatttca
     4021 tcgatggata cccggtctca gggacgggga gatattacta caccgacccc aacatcgtcg
     4081 ggttggtttc cactgaggac atggtggtca tgatggacga gatggggatc gagacgggga
     4141 tcgacgtgaa tcgtctcctg gacattggaa ggatggtgga gaagatcgtc ggccggcgcc
     4201 tgcggtcgga gtgcatccac accggcccga tccccaaagg gcacacggga cgatagtttt
     4261 tcaccaaagt gaaatagaga aggattcata agcaccaaat cccaaataga ttccaatatc
     4321 caaagtccaa tattgccaaa ccacccaatc cccgattttg ggtcattgaa tattgggatt
     4381 tgtttgggat ttgggatttg gtgcttggaa tttattcggt tctttgtggc aaaagggatt
     4441 tgaaatggaa attgccgacc aggacaaata cggtttttgc ttcgcctgcg gtaagaagaa
     4501 tcccatcggc ctcaagctgg actttcgctt cgaagagggc aaggccaaag ccgagttcac
     4561 ccccggccct taccaccaga gctggaacgg gattttccac gggggactcc tggccctttg
     4621 cctggacgag gctttcgggt acgtcctcta tttccaggga atcaagggga tgaccgccaa
     4681 gatggagatg cgcattcgca agacggttca aacgggccag aagatcctgc taaccggaga
     4741 ggtgaagagg aaaacccgga agatcatcga aacctatgcc caggccgaat ttgaagacgg
     4801 aacgctggct gccgaggcca cggccaccat gtacgtgacc gaccgggatt tttgattcgc
     4861 ttccagaaaa atcttggctg gaatgaggga aagtcgctat aatgaggttc gcttcccacc
     4921 ggcagccggc cgaagaagcc aacctgactc ggtcggggac gaaccgccaa aagcggcaag
     4981 ggtccaaggc cgccaacgaa tacctggagg gggttcagac gccttgtatt ttgtgagctc
     5041 cggtttcaaa caaaagttca tagaattttg tctcatggcg agtggaagtc ttttttacca
     5101 tttggtcctt cgacccaagg aatt
//
LOCUS       DASPRR010000159         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2135594, whole genome shotgun sequence.
ACCESSION   DASPRR010000159 DASPRR010000000
VERSION     DASPRR010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135594"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..767
                     /locus_tag="VEL68_06245"
     CDS             489..767
                     /locus_tag="VEL68_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXZ35599.1"
                     /translation="MAVTHVVGKKRGKIMLYALSTCVWCKKTKQLLDQMGVEYDFVEV
                     DLLQGEARTEATEEVKKLNPRCSFPTLSINDQCIVGYDEQRVKEALGS"
     gene            764..1276
                     /locus_tag="VEL68_06250"
     CDS             764..1276
                     /locus_tag="VEL68_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HXZ35600.1"
                     /translation="MREESTVSYEQVERLFQRLHMEAEAGGYHLNPDTPFTKDLVKGL
                     LVNEKRYGYWACPCRLAAGNKEEDLDIICPCDYRDPDLEEYGGCYCALYVTGGVLKGE
                     QKLQSIPERRPPAEERKKPHRGAAMQGLSSLPYPVWRCRVCGYLCAREGPPEVCPVCK
                     AKKDRFERFL"
     gene            1393..2025
                     /locus_tag="VEL68_06255"
     CDS             1393..2025
                     /locus_tag="VEL68_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HXZ35601.1"
                     /translation="MDLFFFLKGFLIGFSIAAPVGPIGVLCIRRTLAYGRRSGLASGL
                     GAATADALYGGVAAFGLTLISHFLIQQQFWFRLVGGVFLLYLGVRAFLSHPESDPPLA
                     RRSDLAGDFLSTVFLTLTNPLTILSFAAIFAGLGLGATGGTYIDAGVMVLGVLFGSAI
                     WWWILSFGVDAFRDRFSLRGLRWVNRISGIIILGFGVAALGSCLAAFYFL"
     gene            complement(2507..3712)
                     /locus_tag="VEL68_06260"
     CDS             complement(2507..3712)
                     /locus_tag="VEL68_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXZ35602.1"
                     /translation="MKAPKAPANQEQIVYDALLRRLMGLLYLYHGVIRITLLRNIALL
                     TYALLTLFHGARGGNGWLSQAALARCLPLQTSAKGREQRLHRFLANPRLTPEVLIPLH
                     VALVLGIQASGAVPLILDQTTIRGIQTLLVGAVFEGRVLPVAFSCFTYRLIRKSQNIL
                     EQSLIVAVMSCFPAESRPLLILDRGYARVALLQKLAQAGIPFLVRAKKNVVVYFQGQP
                     RALSRFPYQPGQIQRHTVFYHSRAKEPLDLIIFRGLGYKETWYLLVPKGFCRNEKEIV
                     DLYAKRMSIEQGFRDWKTHLGVRGLVFRVSDPAPYLTRLLLAFSLSYLICLALGATEE
                     AQKVRAFVEIPRHKPRHQTTRTLSALSIGILRLSLPRFAPKAHEEILILLHILSTGQG
                     LIDYCSAPP"
     gene            complement(3709..4038)
                     /locus_tag="VEL68_06265"
     CDS             complement(3709..4038)
                     /locus_tag="VEL68_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35603.1"
                     /translation="MEDRIPQGLLDLYESLLSAQLRTVRQLKNRKSPKEKPKKKEGMS
                     NMDMAIDILQQARKPLHISEILAQVKAKHRVSLDRESLVSALVKKVHRNQGLTRTAPN
                     TFSATPR"
BASE COUNT         1016 a         1045 c         1304 g         1095 t
ORIGIN      
        1 ccttcctatc ccacctattg aaagtgtatt ccaaatggaa aggttgagtc aatacagaat
       61 ccccttgccc atcctcagtt cttaaaaaag agtccttcga aaataagacc tctggaaaga
      121 aggggggatc tcatggggtt cagggtggag gaatttggat catcatcctg aggcggtggt
      181 tcttccatgg gatcgggcct gtcctggggg gattgaaacg gaaaaatgct ttggctctct
      241 attctgcttg gaggtatctc ctctcaggcc tctacctgcg gaacatggga aggagtttca
      301 tggaaggata agcacccgcc gagcctgatg atccaaattc ctccgccctg aaccccaata
      361 agcacgaaaa actggggggc gggcaagagg ggattctgta ttgactcaac ctttccattt
      421 ggaatacact ttcaatagat gggataggaa ggaccagcgc tcaaaaaaat tccggaggtc
      481 gggcggatat ggcagtgacc catgtggttg gaaaaaaaag aggaaagatc atgctctatg
      541 ccctgagcac ctgcgtctgg tgcaagaaga ccaaacagct gctggaccag atgggggtgg
      601 agtatgactt cgtcgaggtg gacctcctcc aaggggaagc acggacggag gcaacggagg
      661 aagtgaagaa gctgaacccc cggtgttcct ttccgaccct ctcgatcaac gaccagtgca
      721 tcgtgggcta tgatgaacag agggtcaaag aggcgctggg atcatgaggg aagaatctac
      781 cgtttcctat gagcaggtgg agcggctttt tcagcgactg cacatggaag ccgaggccgg
      841 cggataccat ctcaacccgg atacgccctt caccaaagac ctggtcaagg gcctgttggt
      901 caacgaaaag cggtacggat actgggcctg cccctgccgt ctggctgcgg gaaacaaaga
      961 ggaggacctg gacatcatct gcccctgcga ctaccgggac ccggacctgg aagaatacgg
     1021 gggctgctac tgcgccctct atgtcactgg gggggtcctg aagggggagc agaaactcca
     1081 atccatcccc gaaaggcgcc cccctgctga ggagcgaaag aaaccccatc gcggggcggc
     1141 aatgcagggg ctttcctctc ttccttatcc tgtctggcgg tgcagggtct gtggctatct
     1201 ctgcgcccgc gaggggccgc cggaagtatg cccggtatgc aaggcaaaga aggatcggtt
     1261 tgaaagattt ctctgaaaga acccacaaag atgccatcca atagcagggg aaggttacga
     1321 acccgcccct acgaaggctt cccaaacagc cccacctgct gattcctggc ttcaaaggat
     1381 ctaagaaaat tgatcgacct ttttttcttt ttaaaaggat tcctcattgg tttttccatc
     1441 gccgcacccg ttggcccaat cggtgtcctg tgtatccggc ggaccctggc ttatggccgc
     1501 cggtccggcc tggcttctgg gttgggcgcc gccacggcgg atgcccttta tggaggtgtt
     1561 gccgcttttg gcttaaccct catttcccat tttttgatcc aacaacagtt ctggtttcgt
     1621 cttgttggag gagtttttct tctctacctg ggagtccggg cattcttatc ccacccggaa
     1681 agcgatccgc ccttggcacg ccgatcagac cttgcgggcg atttcctctc tacggtcttt
     1741 ctcactctta ccaatccgtt gaccatttta tccttcgctg caattttcgc cggattgggg
     1801 ttgggagcca cgggagggac ctatatcgac gcaggagtga tggttctcgg cgttcttttc
     1861 ggatcggcga tctggtggtg gattttgagt ttcggagtcg atgctttccg cgatcgattc
     1921 agccttcggg gtttgcgctg ggtcaaccgc atctccggca ttatcatctt ggggttcggg
     1981 gtggcggccc tcggttcttg tcttgctgca ttctatttcc tttaagtctt ggagaagaac
     2041 ctttcccccg gaacagcgga ttcacccagg agttgccttt tgattctgca atctgaaact
     2101 ggccttgccc gtcttgactt ttcgccaagc agaatagaga tcgattgaat ctcaggcatt
     2161 gccggaccac aagcttgttt ctcaacagaa aagaggatcg caaaagtgga ggtgggtaag
     2221 gggaacgagg cttgcccccc ttcacttttg gggaaggaaa ggatgagttt acagctgggt
     2281 gaggcaggat tctgtggttg tatatggggg tttgggggtt gagggatttt ttttaagagg
     2341 gagcaggaat gttgaaagaa ggaaagcgga tggaaagata aggttagtca tccaccgatg
     2401 ccctgattca gagggtaggc atagtttttc ctgtacggga gatacatgga tcgttccgtt
     2461 gggaaaaaga aggcgttgat tggatcgatg cgggtgaaga gagcacttag ggtggggcgg
     2521 agcaatagtc aatcagacct tggccggtgg agaggatgtg gagaaggatc aagatctcct
     2581 cgtgtgcttt tggggcgaat cggggtaaag acagtcgcaa gataccgatg gaaagggcgg
     2641 aaagggttcg ggtggtttgg tggcggggct tgtgtctggg gatttcaaca aaggctctga
     2701 ctttctgggc ttcttccgtg gcccctagag ccaaacagat caagtagcta agcgagaagg
     2761 ccagaagcag gcgggtcagg tagggggcgg ggtcggagac ccgaaagacc agcccgcgca
     2821 cgcccaggtg ggtcttccag tcccggaatc cttgttcgat ggacatgcgt ttggcataca
     2881 ggtccacaat ctccttttcg ttgcggcaaa aacctttggg taccagcaga taccaggttt
     2941 ccttgtaccc caggccccga aagatgatga ggtcgagggg ttctttggct cgggaatggt
     3001 agaagacggt atgtcgttgg atttggcccg gctgataggg aaaccggctc agggctcggg
     3061 gttggccctg gaagtaaacc acgacgttct ttttggcccg caccaggaaa ggaatgccgg
     3121 cttgggccag tttttggagt agcgccaccc gggcatagcc ccggtccagg atgagaagcg
     3181 gacggctttc ggcagggaaa caactcatga ccgccacgat aagagactgc tccaggatat
     3241 tctggctttt tcggatcagg cggtaggtaa aacaggagaa agctacgggc agtactcggc
     3301 cctcaaagac ggctccgacc aggagggttt gaatgccccg aatcgtcgtc tgatccagga
     3361 tcagggggac cgcgccgctg gcttggattc ccaaaaccag agccacatgg agagggatca
     3421 agacttccgg agtcaggcgg ggattggcca ggaaacggtg caaccgttgc tctcttccct
     3481 tggcactggt ttgcaggggg aggcatcggg ccaaagcggc ctgggaaagc catccgttgc
     3541 ccccccgggc cccatggaat aaagtcaaca aggcgtaagt caggagagcg atattcctaa
     3601 ggagggtgat ccgaatcacg ccgtgataga gataaagaag gcccatcagc ctgcgcagca
     3661 gcgcatcata gacaatttgc tcctggttgg ccggggcctt cggcgccttc acctgggggt
     3721 agccgagaag gtattgggag cggttcgggt taatccctgg ttgcggtgga cctttttgac
     3781 cagggcactg accagggatt cccgatctag gctcacccgg tgcttggctt tcacttgggc
     3841 caggatctcg gagatgtgga ggggttttcg ggcttgttgc agtatgtcta tcgccatgtc
     3901 catattagac ataccttctt ttttcttcgg tttttccttt ggggacttgc ggttttttag
     3961 ttgtctgacc gttcggagtt gcgctgatag caatgactcg taaagatcca ggagaccttg
     4021 ggggattctg tcttccatgg gtatcctcct tggtgggttt aagaggagga taacaggtac
     4081 gtgtaggtat gtcaagataa aaaatacata cagatcaata tttcttttgc tccacttttt
     4141 tgaaaaattc caaaaagttc cccaaaagtg aaggggggca agatcctttt tcccgttgac
     4201 aaatttttat aaattattca gaattgtcct tgaaagaaaa ataggttgca atattttttt
     4261 caccaacata tttcttagct taactcgttc ggagatgcag tgaaaagaaa gccgtcctca
     4321 atttttgtgg ttttggtaat tggattattt tttcttttat tgccctcttt tatcggctac
     4381 caagaagtca tagaaggtta ctttttttta tctgtggtaa aatatgaaga tcgcgatata
     4441 gaagattttt cacttgataa
//
LOCUS       DASPRR010000160        10212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_527767, whole genome shotgun sequence.
ACCESSION   DASPRR010000160 DASPRR010000000
VERSION     DASPRR010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527767"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VEL68_06270"
     CDS             <1..274
                     /locus_tag="VEL68_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HXZ35604.1"
                     /translation="LTMVREGNKPRYPTVPARLGAEEAEVQVWNNDVLKLAPELIGLR
                     GSATNVKRIFAPERKMGEILGDGEKDPQGAVQLLVQKLVERDLLSF"
     gene            311..1654
                     /locus_tag="VEL68_06275"
     CDS             311..1654
                     /locus_tag="VEL68_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HXZ35605.1"
                     /translation="MSETESPNVPKPKKPRGKAQLMKKCAACGGRCMAVCPVDAIVLD
                     ESGEAQIDMEKCIGCGKCMPACPVEALAKVYTPEEQAFLEKVGQREIPPEDAVPPVDE
                     SFRGVWVFVEQIDGAVAGVSWELMGAGAKLAADLETDLCAFILGDQVEHLAEEAFSYG
                     AKKVYLIDAPVLRHYRTKPYLKGTYDLVRKYKPEIVLMGATGLGRDLAGAAATALRSG
                     LTADCTGLTIDKANRLLEQTRPAFGGNIMATILTRAHRPQMASVRPHVLPKPEPVPGQ
                     KGILIREPINLKEADVGVKILEILQDGQDAGKVDIAGAEFLISGGRGMVSSENFGLLK
                     ELADLLGGTVSASRAAVDAGWMPYERQVGQTGKTVRPKIYVACGISGAIQHLVGMQDS
                     DLIIAINRDKDAPIFEVADLGIVGDVFQVVPALIAKIRELKSGPSRPGAYPEVSA"
     gene            1758..3767
                     /locus_tag="VEL68_06280"
     CDS             1758..3767
                     /locus_tag="VEL68_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ35606.1"
                     /translation="MSNTENVFFAILFAVLALTFIYSAVRLVRLITLGQPDTRLQGGW
                     FKRFLTMLLYAFAQRRVISKPFGFNHLFLFWGFLILFLSNAEFLLNGLFPPFSFRFLG
                     PSLYGLLSLAFDLVSLVVLVCVGIALFRRLVLQPSYIDAKSADALLILSLVAGLMIAY
                     FGFHGCEFALQSEPWPYSMPVSQNIAAPLMLALFGGSLPLAERVFWWVHALIFLGFLN
                     YLPHSKHLHILTAIPDCFCRSFESAALVPRETYSPGKSYGVSRVDEFTWKDLLDFTAC
                     TECGRCNDNCPATLSGKTLNPRYVIQDGKINLKANGEKILHGNVTQGLMPLIRETDES
                     PGSVGEDALWACTTCRACMSNCPVFIEHVPKIIKMRRHLTETQAKFPEELRNFYESVE
                     QRSNPWGIIPQDRTKWAKGLEIPLLAADESVEYLFYVGCAGSFDSRNRKAVTALVQAL
                     RAAGVSFGILGAEEKCCGDSLRRLGNEFIFEKMTAENVELFKKYKVKKILTYCPHCYS
                     TLKHDYRQFGLEATVLHHSEFLYDLLRAGKLQLKGEDSLGRVVIHDSCYLGRYNQIYR
                     QPREVIRMATGQKPVEMDRKLEKSFCCGAGGGRMWMEESAEYRLNINRTRQALAKSPG
                     TIAVSCPYCMTMFEDGLKNEKAGQVRVLDLAEIIAPRIVFPQSPG"
     gene            complement(4020..5921)
                     /locus_tag="VEL68_06285"
     CDS             complement(4020..5921)
                     /locus_tag="VEL68_06285"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ35607.1"
                     /translation="MMEEEKNMGSPAVGSSAWGRLLFNVSDAVDRACTFSFVMATIAF
                     GLTMLGGVFFRYVLNQSLTWSDEVALIFFIWATLLSIASGYLHDKHVNLDLIIRKLPP
                     DWEAGVSVAAEGLTLGYLISLTVSSIQHLPLVAEMITDALRLPITILFSSIPVACLIM
                     DLHWARRNLAVGKAWMAVVKLIIAAVFVSLVMLPLGQFVQLTGISRAIVMLIALFGPM
                     LIGVPVAISLGFMATFYVGAVGDVPFNVGAEQIYNGVSIIALMAIPLLMLAGKLMHEA
                     GVARYIVDFAQVLVGRVRGGLGAANVVASFLFGDISGSAVSDTAAIGSLMIPQMKERG
                     YQPAFCAALQGTAGTLGMMAPLAITILMYATATMASVSRLAAATILPAFLLAGSFMLV
                     VLFHARRHNYPREHVPRELILPRTFRALPGLFALVLLVGGILGGVFTPAEVGSVLVGY
                     VMVLSFFYKTAQPRRLYRAVVEAGYISGMTLFMASTSSFLGFMLARDLVPHHVVNFIS
                     GISTDKNAVIFIVSGVFIVLGMILEPPAMTFGFLPSFMPLLSKVGVDIVHWGVLFCTN
                     MGLGCIVPPVALNLFISTQLAGVRYGDAVRACLPFIIIMTIDLVIMAVFPIIPMMIPH
                     LLFGYPLPK"
     gene            complement(6112..7164)
                     /locus_tag="VEL68_06290"
     CDS             complement(6112..7164)
                     /locus_tag="VEL68_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ35608.1"
                     /translation="MAKMFVNRGTVISIILACTFMVFTAIPASAAGPVVLRAGASDPE
                     GTTAPALSIGKLCELATQLSKGELNVQPFYQSLGVEQQLAAGIKAGTIDIGFTATPNL
                     APFTDAYLQFDLPFLFKNDRAYIDVLENHAAGKKALAQFEKDLGVKVLLITSHTYDAP
                     VSGTDLMLRSKPAKVPADLKGLKIRTSSTPVEINLIKAYGANPTPIPYGQLYTALQQG
                     VIDGNAATPLPPAASIKLYEVTKYYTAIGFRFNLLPIYMNRKKFDSLTASQQKAILDA
                     AAQVKPLAGQWARDKVQSSITEYEKAGVPIYHPTKEEMAQWLAVREKVWQEIGDAFKG
                     KIDLNVANDIFKLRPQ"
     gene            complement(7496..8455)
                     /locus_tag="VEL68_06295"
     CDS             complement(7496..8455)
                     /locus_tag="VEL68_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HXZ35609.1"
                     /translation="MERSSGERGTVFNITKYMLEDGPGIRTVVFLKGCPLRCLWCSSP
                     LCQTTEPSLVYLKYKCISCGACLPACPNQSLFEDEEGKIGRNFERCTHCGACAKVCPA
                     GAREIRGSTMTVEEVLEKVEKDRVFYRRGGGGVTLSGGEILMQAAFATNILHRCWDKL
                     IHTAVETCAFGSWEDLRAILTYTNLAFIDIKHSDPLIHKKLTGRSNRQILDNIRKAAA
                     YCLESNRHLILRFAVVPGMNDSRENLRDIADFLKSLPGGWELNLLPYHKYGVSKYDWL
                     GMEYKLVDVEPPSREQLLEIVRYFESFGILCSLGGAEIRSVVA"
     gene            complement(8632..10209)
                     /locus_tag="VEL68_06300"
                     /pseudo
     CDS             complement(8632..10209)
                     /locus_tag="VEL68_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013399.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
BASE COUNT         2174 a         2932 c         2935 g         2171 t
ORIGIN      
        1 cctcaccatg gtgcgggagg ggaataagcc ccggtatccc acagtcccag cccgtctggg
       61 agcggaggaa gcggaggttc aggtctggaa caacgacgtc cttaaactgg ccccggagtt
      121 gatcggcctg cggggttccg ccaccaatgt gaagcggatt tttgccccgg aacgcaagat
      181 gggcgaaatc ctgggcgacg gggagaaaga cccgcaggga gcggtccaac ttctggtcca
      241 gaagctggtc gagcgggacc tcctttcctt ttgaggcccc tcaaccgact tttaagggag
      301 tcatgaaatt atgagcgaaa cagaatcccc caatgttccc aagcccaaga agcctcgcgg
      361 caaagcccag ttgatgaaga aatgcgccgc ctgcggcggc cgctgcatgg ccgtctgccc
      421 ggtggacgcc atcgtgctgg acgaaagtgg ggaagcccag atcgacatgg agaagtgcat
      481 cggctgcggc aaatgcatgc ccgcttgtcc cgtggaggcc ctggcgaaag tctacacccc
      541 ggaggagcag gctttcctgg aaaaagtggg gcagagagag attcccccag aggatgcggt
      601 tcccccggtg gatgagagct ttcggggcgt ctgggtcttc gtggaacaga tcgacggtgc
      661 tgtggcggga gtcagctggg aactgatggg ggcgggggcc aagctggccg cagacctgga
      721 aacggacctg tgcgccttca ttctggggga tcaggtcgaa catctggccg aggaagcctt
      781 ttcctatggg gcgaagaaag tctacctgat cgatgccccc gtcctgcgcc attatcgcac
      841 taagccgtac ctgaagggaa cctacgacct ggtccgcaag tacaaacccg agatcgtgct
      901 catgggcgcc acgggcctgg gaagggacct ggcaggggcc gcggccaccg cgttacggag
      961 cggtctcact gccgactgca ccggattgac catcgataaa gccaaccgac tcctcgaaca
     1021 aacgcggccg gcctttgggg ggaatatcat ggccaccatc ctcacccggg ctcaccgtcc
     1081 ccaaatggcc tcggtccgcc cccatgttct tcccaagccg gaacccgtgc ccgggcaaaa
     1141 gggaatcctg atccgagagc caattaacct gaaggaagcg gatgtgggcg tgaagatcct
     1201 ggaaatcctc caggacggtc aagatgctgg gaaggtagac attgccgggg cggaattctt
     1261 gatctccggc gggcggggga tggtgtcctc ggaaaatttc ggccttctca aagaactggc
     1321 tgacctactg gggggaacgg tcagtgcctc ccgggcggca gtcgatgccg gatggatgcc
     1381 ctacgaaagg caggtgggcc agaccgggaa gacggtccgc cctaaaatct acgtcgcctg
     1441 cgggatttcc ggggctatcc agcacctggt gggcatgcag gattcggatt tgatcatcgc
     1501 catcaaccgg gataaagacg cccccatctt cgaagtggcg gacctgggca tcgtggggga
     1561 cgttttccag gtcgtcccgg ctttgattgc caaaattcgg gagctgaaga gcggtccctc
     1621 acgcccgggc gcctatccgg aggtctcagc ctgagaaatt ccccggccca gtgagcggcc
     1681 cgatcaggga tcgggcccta caatttaaaa aatgaatgta gggcgcgttc cccgaacgcg
     1741 ccgattaaag gaaaaaaatg tccaacacgg aaaatgtttt ctttgcaatt cttttcgccg
     1801 ttctggccct cacctttatt tattcggccg tccgcctagt ccgcctgatc accctgggac
     1861 aaccggacac ccgcctgcag ggcgggtggt tcaaaagatt tctgaccatg cttctctacg
     1921 cctttgctca aaggcgcgtc atcagcaaac ctttcgggtt caatcacctc ttcctctttt
     1981 gggggttttt aattctcttc ctttccaacg ccgaattcct ccttaatggc ctctttcccc
     2041 ccttttcctt ccgcttcctg ggcccttctc tttatggcct cttgagtttg gcttttgatc
     2101 tcgtctccct ggttgtcctg gtctgcgtgg gaatcgccct gttccggcgt ctggtcctcc
     2161 aaccctccta cattgacgcc aaaagcgccg atgccctgct catcctgagc ctggtggcgg
     2221 gtctgatgat cgcttatttc gggttccacg ggtgcgaatt cgccctccag tctgagccct
     2281 ggccctattc gatgcccgtc agccagaata tcgcagcccc tctaatgctt gctcttttcg
     2341 gcgggtcgct ccccttggcg gagcgtgtct tctggtgggt ccacgccctc atcttcctcg
     2401 ggttcctgaa ctatcttccc cacagcaagc acctgcacat tctcaccgcg attccagatt
     2461 gcttctgccg ttcctttgaa agcgcggccc tggttccccg ggaaacctac tctcccggta
     2521 agtcctacgg ggtctcccgc gtcgatgaat tcacctggaa ggacctgctg gacttcacag
     2581 cctgcacgga gtgcggccgc tgcaacgaca actgtcccgc gacgctgagc gggaagaccc
     2641 tgaatccccg atacgtcatc caggacggga aaatcaacct gaaggccaac ggggagaaaa
     2701 tcctccatgg aaatgtcacc cagggtctga tgcctttgat ccgagagacc gacgagtcgc
     2761 cggggagcgt tggggaagac gcactctggg cctgcacgac ctgccgggcc tgcatgtcca
     2821 actgcccggt cttcatcgag cacgttccca agatcatcaa gatgcgccgg catttaaccg
     2881 aaactcaagc caagtttccc gaggagttga ggaattttta tgaatccgtc gagcagaggt
     2941 ccaacccttg gggaattatt ccccaggacc gcaccaaatg ggccaagggc ctggagattc
     3001 ccctgcttgc ggcggatgaa tccgtggagt atctctttta cgtgggctgc gccgggtctt
     3061 tcgacagccg gaatcgcaaa gcggtaacag ccctggttca ggcgctgcgg gctgcgggag
     3121 tctccttcgg aatcctaggt gcggaggaga agtgctgcgg cgattccctg cgccgcctgg
     3181 gcaacgagtt catcttcgag aaaatgaccg cggaaaacgt ggagctcttc aagaaataca
     3241 aggtaaaaaa aatcctcacc tattgcccgc actgttattc caccctcaaa catgactacc
     3301 ggcaattcgg gctggaggcg accgttcttc accactccga gttcctgtac gacctgctca
     3361 gggccggcaa actgcagctc aaaggggagg actcgctcgg ccgggtggtc attcacgact
     3421 cctgctacct cggccgatac aatcagatat atcgtcagcc ccgggaggtc atccggatgg
     3481 ccaccggaca aaagcccgtg gagatggacc ggaagctgga aaagagcttc tgctgcggcg
     3541 ccggcggtgg acggatgtgg atggaagaga gcgcagagta ccgcctgaac atcaaccgga
     3601 cgcggcaggc tctggccaaa tcccccggca ccatcgccgt ctcctgcccg tactgcatga
     3661 ccatgtttga agatggtttg aagaatgaga aagcaggtca agtgcgggtc ctggacctgg
     3721 cggaaatcat cgccccccgg atcgtcttcc cccaatcccc tggttaagaa atatcccccc
     3781 gggaagccac ctattatcgg aggtttaatg gtatggacgc tggcaactaa gccgttcgtg
     3841 ctgagtcttc ggcgtgagct cagtcgaacg cttgtcgagg catgaacggc ttagcgatgc
     3901 acccttcgac aagcatgtcc tgagcgcgcc gaagggctca gcatgaacgg taacttcttt
     3961 ttgtgttttc ggcgcgatct gcggcgatct ttttggtcat cgagcaatcc ccgtaccggt
     4021 catttaggca gggggtagcc gaagaggagg tgaggaatca tcatcgggat gatagggaat
     4081 acggccatga tcaccaggtc aatggtcatg atgatgatga atggaagaca agcccgcacc
     4141 gcatcgccgt accgaacccc tgccaactgc gttgagatga acaagttcaa ggccacggga
     4201 gggacgatgc acccgaggcc catgttggtg cagaagagga ctccccagtg aacgatgtcc
     4261 acaccgacct tacttagcag aggcatgaag gagggcaaaa agccgaaggt catggccgga
     4321 ggctccagga tcatgccgag gacgatgaat accccgctca cgatgaagat caccgcattt
     4381 ttatcagtgc tgatccccga aatgaaattc accacatggt gcggcaccag gtcccgggcc
     4441 agcatgaaac cgaggaacga tgaggtcgag gccataaaca aggtcatacc gctgatgtag
     4501 ccggcctcca ccaccgcccg gtaaagacgc ctgggctgag cggtcttgta aaagaaggac
     4561 agcaccatga cgtagcccac gagcacggag cccacttccg ccggggtgaa aacgccgccg
     4621 aggattcccc cgaccaggag cactagggcg aagagaccgg gcaaggctct gaaagtgcgg
     4681 ggcaggatca attcccgggg cacgtgctcc cgggggtaat tgtgtctgcg ggcatgaaag
     4741 aggaccacca gcatgaagct tccggccagc aggaacgctg gtagaatggt cgccgcggcc
     4801 agccggctga ccgaggccat ggtagcggtg gcgtacatca ggatcgtaat ggccaaggga
     4861 gccatcatcc cgagggttcc cgccgttccc tggagggctg cgcagaaggc cggctggtag
     4921 ccacgctcct tcatctgggg gatcatcaag gagccgatgg ccgccgtgtc cgagacggcc
     4981 gaacccgaga tgtcaccgaa gagaaagctg gccaccacgt tggccgcgcc caatcctccc
     5041 cggacgcgcc ctacgagcac ctgcgcgaaa tccacgatgt aacgggcgac gcccgcctcg
     5101 tgcatcagct tcccggcaag catgagcagg gggatggcca tcagagcaat gatgctgacc
     5161 ccgttgtaaa tctgctccgc ccccacattg aagggaacgt ctccgacagc gccgacgtag
     5221 aaggtagcca tgaatcccag ggagatcgcc accggcactc cgatcagcat gggcccgaag
     5281 agggcgatga gcatgacgat cgcccgggat atgccggtga gctgtacaaa ttggccgagg
     5341 ggtagcatga ccagggaaac gaaaactgcc gcgatgatca atttgacgac agccatccag
     5401 gcctttccta cagcgagatt acggcgcgcc cagtggaggt ccatgatcag acaggccacc
     5461 ggtatggagg aaaagaggat ggtaatcggc aaacggaggg catcggtaat catttcggcc
     5521 accaggggga ggtgctggat gctcgagacc gtcagggaaa ttaggtaacc gagggtgaga
     5581 ccttccgccg ccacgctcac gccagcttcc cagtccggag gcagcttgcg aatgatcagg
     5641 tcaagattca catgtttgtc gtgcagatag ccggaagcga tggacaaaag cgtggcccag
     5701 atgaagaaga tgagcgcgac ttcatcggac caggtcaagg actgattcaa cacataacgg
     5761 aagaatacgc ccccgagcat ggtgaggcca aaggcaatgg tggccatgac aaaagagaag
     5821 gtgcaggccc ggtccacggc atcgcttaca ttgaaaagga ggcgacccca ggcggaagaa
     5881 ccaacggcag gggagcccat gtttttttct tcttccatca ttcttcttcc tcttcattcc
     5941 tttggcggcg gaaaagcccg gctcgctgcg ggcgccgttt ccccccacaa tttccatttt
     6001 cctgagattt acggatttcc cgaaaatccg agggccccgg aagagacccc tttcttctac
     6061 ctcttccggg gaccgggtct ggttttcagc atgaaagggc ggtaggctta attattgggg
     6121 cctcagcttg aagatgtcgt tggcgacgtt aaggtcgatc ttacccttaa aggcgtcgcc
     6181 gatctcctgc cagaccttct cccgcaccgc caaccattgg gccatttcct ctttggtagg
     6241 atggtagatg ggaacgccgg ctttttcgta ttcggtgatc gaagactgaa ccttgtccct
     6301 cgcccattgc ccggccagcg gcttgacctg ggccgcggca tccaggatgg ccttctgctg
     6361 ggaagcggtg agcgaatcga acttcttccg gttcatgtaa atgggcagaa ggttaaagcg
     6421 gaagccgatg gccgtataat acttggtgac ctcgtagagt tttatggacg cggcaggggg
     6481 caggggggtc gcagcattcc cgtcgatgac cccctgctgg agcgcggtgt acagctgacc
     6541 gtaagggatg ggcgtggggt tggcgccgta ggccttaatc aggttaattt cgactggggt
     6601 gctggaagtg cgaattttca gccccttcag atcggcgggg acttttgccg gcttgctgcg
     6661 gagcatcagg tcggtgccgc tgaccggcgc gtcgtaggta tgactcgtga tcaggagcac
     6721 cttgaccccc aggtcttttt cgaactgggc cagcgctttt ttgccagccg catggttctc
     6781 cagcacatcg atgtaggctc tatcgttctt aaaaaggaag ggaaggtcga actggaggta
     6841 ggcatccgtg aagggtgcca aattcggggt ggcggtaaag ccgatatcaa tggtgccggc
     6901 ctttatgccc gccgccagct gctgttccac cccgagggac tggtaaaagg gttgaacatt
     6961 cagctctcct ttgctcagct gggtcgccag ctcgcacaat ttaccgatag acaaggcggg
     7021 tgccgtcgtc ccctcggggt ccgacgctcc ggctcgaaga accaccggtc cggccgcact
     7081 ggccgggatg gcggtgaaga ccataaacgt gcatgccaag ataatggata tcacggtccc
     7141 tctattaacg aacatctttg ccatcgttca gcctcctttt cattcacttc tgattaaact
     7201 cggtgaatcg ttggtgctgc cccctcatgg ggccggcaga ttcaacttcc aagacttacc
     7261 tgggtcacct ccttccctga ccacctggct gcttaaccgt aacagtccgg agagcgtttc
     7321 taaagggctt caggtctcat tggctaccct tcggatcaga acgtgctttt gggtgagttg
     7381 agtataggaa aacgaggttt cggtgtcaag aaaaaagccc cgcctttatg atcggccggc
     7441 atcttgtttc tttttggcag gctacgtctc tccacgggga aggagaaatg aggactcagg
     7501 ccaccacgct gcggatctcc gcgccgccca agctgcacaa tatgccgaaa gattcaaaat
     7561 acctgacgat ctccagcaat tgctcccgcg aagggggctc cacatcaacg agcttgtatt
     7621 ccatccccag ccagtcgtat ttcgagactc cgtacttgtg gtaaggaaga agattcagct
     7681 cccacccgcc gggcaagctt ttcaagaaat cggcaatgtc ccttaaattt tcgcgggagt
     7741 cattcattcc cggaacgacc gcgaagcgca ggatgagatg cctatttgat tccaggcaat
     7801 aggcggctgc tttccggata ttgtccagta tttgacgatt tgaacgaccg gtgagtttct
     7861 tgtgaatcag agggtcggaa tgtttgatgt cgataaaggc caggttggtg taagtgagga
     7921 tggctcgaag gtcttcccag ctaccgaaag cacacgtctc aactgcggtg tggatgagct
     7981 tatcccagca ccggtggagg atgttggtgg cgaaggcggc ctgcatcagg atttcaccac
     8041 cggaaagggt cactcctccg cctcctcgcc ggtaaaagac gcggtccttt tccaccttct
     8101 ccagaacctc ctccacggtc atcgtcgagc cgcgtatctc ccgggccccc gccgggcaga
     8161 ccttggcaca agccccgcag tgggtgcacc tttcaaagtt gcgtccgatt tttccttctt
     8221 catcctcaaa aagggattgg ttcgggcagg ctggcaagca ggccccgcag gagatgcact
     8281 tatatttgag atagacgagg cttggctctg tcgtttgaca gagagggctg ctgcaccata
     8341 gacatcggag cgggcatccc ttcagaaaaa ccaccgtgcg gatgcccggt ccatcctcca
     8401 gcatatattt ggtgatgttg aaaacggttc cccgctctcc ggaggatcgt tccaaatggt
     8461 tctccccaga cttcttggag taatgttctt tttccacctc aggcaacctt ggcgatcctt
     8521 cctcaccgtt tccgattgga aaagtcgagg ctcagggaga aagaaaattc cttcccccga
     8581 tcaagatttc ggatgggaca tttagcgggg acaggaatgg ctcaaatctt cttaattgaa
     8641 ccgatgctcg gagcgggaaa taatatcgtc ctgtatgtct ttcccgaggt ccgtgaagta
     8701 agcgctgtaa ccggccaccc ggaccatgag cgtggggtaa ttctcgggat gtttttgggc
     8761 atcgatcaag actgctttgt tgatgcagtt gaactgaata tgggcgatgc ccagatcatt
     8821 caaagccgtg cggatcaggc tggcaaactt tttcagccct tctctcttga aatgcaaatc
     8881 gctgaacctg tcatacaaga gatcactctc ttgcggttcc gggctgaatt tcctgctggc
     8941 tttcatgata cctcctcttt ggtaagtctc cgcttatcgc tccgcgaccc cctgggccta
     9001 gttggcgacc agttcggact tggaaatcca catgtttaat atcgtcccgt tggtctgccc
     9061 ggcatggtcc agcttgccgt aagacttgat cgccgcggtc ggtccttctt tatccgtatg
     9121 ctgctctgcg gaaatcgtgt cggccagggg gacgcaggcc ttcttgccat tgggctgggc
     9181 ccaggttttc ttgccgaagc ttacgttcgc cgcaaccatt tggatggatg ggtccatata
     9241 atgacccagg tacgtcctat attttttggt ctccagggcg taaaagtcca gcacctcacg
     9301 ggccagggag tccggcccgt ccaggtcgtt cccgtacttg ggcgaatttc tgagcttctc
     9361 cctcaaccgt tcatagccct cgaaattggc gtcgatggcg gttaagagtt cgtccatggt
     9421 gatctctttt ttctcaaaga ccattgtttt gatgttgtac agggagttgg ccacatcggc
     9481 ggtggccaaa acggcgatga ccgggccaac actcacaggg gccccgcctt cctcgacgtc
     9541 caggcccttc tctattgtcc ccttggtcaa gatcgtgtaa accggtttga aatgctcctc
     9601 ggaccggatc ttctcgccca cgatcatgtg ggcatgagcc tctttgatcg cgtaagccag
     9661 ctgcaccttg aaaacattga aaaactcttc aaaagtcttg aaatcccgag gatcgcccgt
     9721 cttggggccg cactgcatcc cgccccccca gcccggagcg cctttgggtt tgaccccgtt
     9781 gctcaacacc aggctcatca ccgcgctcat attgagcatg caactgtccg tatggccgta
     9841 ctgcatccct tgaaccccgg gttccacgca accgatatag gccccatatt cccgtatctg
     9901 gtcgaaggag tacttgcttg gaccattggg cccggcggcg cggcccatga gctgctgcca
     9961 gagggtttcg cagttgaaaa aactgggatg gcccatcccc tgcgcggcga gcatgacgcc
    10021 ctcataaagc agatcatccg gggtcttcga gtgaagcaac aaggctaccg aaggggaggt
    10081 ggtgtgaact tcccgcatgg cctggatggc caggtaactc accttgttgg aggcatcgtt
    10141 gccatatcgg tccacccctc ccacatcgag ctgctggaac cggaaataac cgcgcccgtt
    10201 gatcgccagc tg
//
LOCUS       DASPRR010000161         4058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_356263, whole genome shotgun sequence.
ACCESSION   DASPRR010000161 DASPRR010000000
VERSION     DASPRR010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356263"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VEL68_06305"
     CDS             complement(<1..219)
                     /locus_tag="VEL68_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ35610.1"
                     /translation="MRLKDKVAIITAAGSGSGRAGSVLFAREGARVVVGDIDPKGGQE
                     TVKLVKQAGGEAVFVPVDCGKVADMRKLI"
     gene            complement(248..952)
                     /locus_tag="VEL68_06310"
     CDS             complement(248..952)
                     /locus_tag="VEL68_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35611.1"
                     /translation="MLKVEAIDIYYGDLQAVKGVSFQVGKDQIVSLVGSNGAGKTTTL
                     NAISGLHRLRSGSIHFGEINISRRGSHQIVELGIIQVPEGRMLFPEMTVMENLEMGCY
                     SLRARRNFQDSLQEVIGLFPILAERKQQLAGTLSGGEQQMLAIGRGLMSQPTVLMLDE
                     PSLGLAPFVVQEIIEVIKTIRQRGTSVLLVEQNVFHALSISDRACILENGEIVMEGKG
                     EDLLKNEKVRKAYLGL"
     gene            complement(945..2750)
                     /locus_tag="VEL68_06315"
     CDS             complement(945..2750)
                     /locus_tag="VEL68_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXZ35612.1"
                     /translation="MVIQNSLQRIRHPKVYLTLIAIGALYSIALSVKNPYWGDILTMT
                     FLLGALSLSWNILGGYGGQFSLGHAGFFGIGAYTSTLLLIQYHVNPWIGMLLGGILAA
                     AIGGVVFYPCFRLKGIFFCLATLAFQEVVKISAVHFRGLTGGAMGISLPFKAGLTNMM
                     FQGKTGYMLISLSFMIIIALVTLAMERSRFGYKLVALREDEDATETLGISTSRCKLLA
                     TMISAFFAGCLGTFYAHYSSLVDPHSVFDMMLSVEFVIFAILGGVGTVLGPILGAFAL
                     VPVDALMRGYLGQGQQGVSFFAYGMLLTGVVIFLPQGILSWISSPVNRLFQTLSGEKP
                     RPEPRPDKIEAVAEKAERTSRLMTTASILEIAGVDKSFGGLKVIQSLDFHVREGEIVG
                     LIGPNGAGKTTCFNLITGFEEPDSGKILFKGEDITGVKPPNRISKRGVARTFQIVKPL
                     AGLTVTENVLAGAAERAGSIHEARVKSRKIIDLLGLWEFRDIKASSLTLGGRKRLELA
                     KALATDPTLLLLDEVMAGLNPTEVEEAIRMIRRISSRGVTLLVIEHVMKAVMSLADRV
                     VVIHHGQKICEGRPQDIIRDPRVIEAYLGKGFQYA"
     gene            complement(2770..3633)
                     /locus_tag="VEL68_06320"
     CDS             complement(2770..3633)
                     /locus_tag="VEL68_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35613.1"
                     /translation="MIGQVLVNGILLGGIYALVSLGLTLIFGVMRIINFAHGQFLMLA
                     MYATFWLFQLYGVDPYISILIVVPLMFVIGIAAYRMIIQPIIDASEMTHVFATLGLNL
                     SLQGIALFLWQGDFRGIRTSYSSMLVHLGPLYINIPRFIIFLSAIATILLLFLFLKKT
                     YTGKAIRASAQKRVAAQLMGVNLSKIYMIAFGIGIAIVGVTGAVLMPIYEVFPSVGSL
                     FALVAFVVVVLGGLGNLGGALVGGIIIGVVESVSGVFIAPALKEGVYFIIFVIILLLR
                     PTGIFGKAVIH"
BASE COUNT         1016 a         1157 c          992 g          893 t
ORIGIN      
        1 aatcagttta cgcatatcag ccaccttgcc gcaatcgacg gggacaaaaa ccgcttcacc
       61 gccagcctgc tttaccaatt tgacggtctc ctgcccccct ttgggatcaa tgtctcccac
      121 caccaccctt gctccctccc gagcaaatag caccgaccct gcacgacccg accctgaacc
      181 tgctgctgta ataatggcta ccttgtcttt gagtctcatt tttctcctcc ttttttaatt
      241 tcatcctcta caacccgagg taagccttcc tcactttttc attcttcagg agatcctccc
      301 cttttccctc catcacgatt tcgccgtttt ccaggataca ggcccggtcc gagatggaaa
      361 gggcatggaa gacattctgt tccaccagca aaacggaggt gcccctctgg cgaatggttt
      421 ttatgacttc gataatctcc tggaccacga agggcgccag acctagggag ggctcgtcca
      481 gcatcaggac cgttggctgg gacattaacc ctcttccgat ggccagcatc tgctgctctc
      541 cgccgcttaa ggttcccgca agctgctgtt tcctctccgc cagaatggga aagagtccga
      601 tgacttcctg gagggagtcc tggaaatttc tccgggccct caacgaataa caacccattt
      661 ccaggttctc catcaccgtc atttcgggga aaagcatgcg tccctcgggg acctgaatga
      721 tacccaactc gacaatttgg tgcgatccgc gccgactgat attgatttcg ccgaaatgaa
      781 tcgaaccgga acgaagacga tggagtcccg aaatggcgtt cagggtcgtt gtcttccccg
      841 ccccattgga acccaccagg gatacaatct ggtcttttcc cacctggaaa gagactcctt
      901 tgaccgcctg gaggtctccg taatagatat caatcgcctc aaccttaagc atactggaat
      961 cccttgccta gataggcctc gatcactctg gggtcgcgga tgatatcttg aggccttcct
     1021 tcgcatattt tctggccgtg gtgaatcacc acaacccgat cggcaagact catcaccgct
     1081 ttcatgacat gctcaatgac caagagggtg acgccgcggc tggaaattct tctgatcatt
     1141 cggatggctt cttcgacttc ggtgggattc aaaccggcca tgacctcatc caacaataag
     1201 agggtcgggt ccgtggccaa agccttggcc aactccaacc gcttgcgtcc tcccaaggtc
     1261 aaggaggagg cttttatatc tcgaaattcc cacagaccca gcaggtcgat gatcttcctg
     1321 cttttcaccc tggcctcatg aatgctgccg gctctctcag ccgccccggc aagaacattt
     1381 tccgtcaccg tcaggccggc gaggggtttt acgatctgaa atgtccgggc aacgcctctt
     1441 ttcgaaatcc ggttcggcgg ctttacaccg gtgatatcct cccctttgaa aaggatcttc
     1501 ccggaatctg gttcctcgaa accggtgatg aggttaaaac aagtcgtttt ccccgcgccg
     1561 ttgggcccga tcagacccac gatttcaccc tctcgaacat ggaaatcaag ggattgaatg
     1621 acttttaatc cgccaaagct tttgtccacc ccggcgattt ccaggatgga agcggtcgtc
     1681 attaaccggg aagttctttc cgccttttcg gccacggcct caatcttgtc gggacggggt
     1741 tccggtcttg gtttttcccc ggaaagggtc tgaaacagcc ggtttacggg actcgaaatc
     1801 caggacagga tgccctgggg taaaaagatt accaccccgg tcaagagcat gccataggca
     1861 aaaaaactca ctccctgttg gccttgcccg agataccctc tcatcaatgc atcgaccgga
     1921 actagggcaa acgcccccag gatcggaccc aggactgttc ctacccctcc caggatggcg
     1981 aagatcacga attccaccga cagcatcatg tcgaaaaccg agtggggatc gacgaggctg
     2041 ctataatggg cataaaacgt ccccaggcac ccggcaaaaa aagcactgat catggtggcc
     2101 aggagcttgc atctggaggt actgattccc aacgtctcgg tggcgtcttc gtcttccctc
     2161 agggcaacca gtttataccc gaaccgggac cgctccatgg ccagggtgac cagggcgatg
     2221 atgatcatga agctcaggga gatcagcatg taccctgttt tcccttgaaa catcatgttg
     2281 gtcaggcccg ccttaaaagg gagggagatc cccatggccc ctccggttaa ccctcgaaaa
     2341 tggacagcgg aaatctttac cacctcctgg aaggctaagg tggccagaca gaaaaagatt
     2401 ccttttagcc ggaaacaggg ataaaatacg accccgccta tagcggcagc caatatgccc
     2461 cccagaagca tcccaatcca tgggttgaca tgatactgga tcaaaagcag cgtggaggta
     2521 tatgcaccaa tgccaaaaaa cccggcgtga cccagggaga attggccgcc ataaccgccc
     2581 agaatattcc aggagaggct taaggccccg agcagaaagg tcatcgtcaa gatatcgccc
     2641 caataggggt tctttaccga aagggcgatg ctgtacagag ccccgatagc aattagcgtc
     2701 agataaacct tcggatgacg gatcctttgt aaggagttct gaataaccac ggaacggttt
     2761 tcccttcggc taatgaatga ccgctttccc aaaaattcca gtgggtctca atagcagaat
     2821 aatgacgaaa ataatgaagt agaccccttc tttgagcgcc ggagcgataa atacgccgct
     2881 caccgattcg accacaccga tgatgattcc gcccactaac gctcccccga ggtttcccag
     2941 cccccccaag accacgacca caaaagcaac cagggcaaat aaagacccaa cgctggggaa
     3001 gacctcatag atgggcatta aaaccgcccc cgtaaccccg acaatggcaa ttccaatccc
     3061 aaaggcgatc atgtagatct tactcagatt cacgcccatc aactgggccg cgaccctttt
     3121 ttgagcactg gcccggatcg ctttgccggt ataagtcttc ttgaggaaaa gaaacaggag
     3181 caggatggtg gcgatggcgg acaggaagat aataaaccgg gggatgttga tgtagagggg
     3241 gcctaagtgg actagcatgg aagaatagct ggtgcgaatt ccccgaaagt ctccctgcca
     3301 taaaaaaagg gcgatcccct gcaaagacag atttaacccc aaagttgcaa atacatgggt
     3361 catctccgag gcatcgatga tgggttggat gatcatgcgg taggccgcga tcccgatgac
     3421 aaacatcagg gggacaacga tgagaatcga gatgtagggg tccactccat agagctgaaa
     3481 gagccagaag gtggcataca tggccagcat gagaaattga ccgtgggcga agttgatgat
     3541 tctcatcacg ccgaagatca gcgtaagacc caggctcacc agggcgtaaa tgccgcccag
     3601 aagaatgccg ttgaccagaa cttgaccgat catagaaccc ttttgtggaa tggacggaag
     3661 gattcattct tccctcttgc ctgaaggtgt gaaaaattac ctaaaatcac ccccaccctc
     3721 gccctccccc gtcgaggggg aggaaatttt taaaaactat ttcaatatgg ttaccctctc
     3781 ccctcgcggg agagggtagg gtgaggggga atatttcaat tttttcacag cttccctgtg
     3841 cagggggaag aatggctccc aactcaaacc gtcaaggaaa ttacttcata gccctttgtc
     3901 cccacagggg catggggagc atcagctcgc ttttggcgaa ttctggggga taaaccagat
     3961 gaccgacact tcctttttgc cactgaaaca cattggggta gccgatcaga ttcgcccctg
     4021 cattcttgtg tccttgggga gccgcctgat acccaaag
//
LOCUS       DASPRR010000162         5631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1977124, whole genome shotgun sequence.
ACCESSION   DASPRR010000162 DASPRR010000000
VERSION     DASPRR010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977124"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..809)
                     /locus_tag="VEL68_06325"
     CDS             complement(156..809)
                     /locus_tag="VEL68_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35614.1"
                     /translation="MEGKRERRFLGTRAILLVMVAGFGFGCAGLQPAEKQKVLTPTDR
                     GMVLFLDKYEFKRPPQNWALMKNLEGGDFELGFLLIERGPFPSQTTFMYDEEPYGSSP
                     DLENRAKYYCTRFLFNSGIDLRVSKPEKTTLGGRDALAIYMEGENPNRDEKAKSKIYL
                     LKKGERIVSFVCTMWRPMNGTYAQEPFDYFEDFAQSFKFLKKDFYENFEDELRKAGL"
     gene            complement(824..1165)
                     /locus_tag="VEL68_06330"
     CDS             complement(824..1165)
                     /locus_tag="VEL68_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXZ35615.1"
                     /translation="MDGHNRDLYLYKRLNAQVLGVSKDDPITNKFFAESLALEFPIIS
                     NVLGFMAKAYGAYSEKPPFGPDGSINTFGRRTVVIDKKGIVRYLKDGSPNNKEILDLL
                     LKLEKESRGLK"
     gene            complement(1175..1393)
                     /locus_tag="VEL68_06335"
     CDS             complement(1175..1393)
                     /locus_tag="VEL68_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35616.1"
                     /translation="MPRKRLLFMTLIAVPIFLVLGMGYGWAQLKVGDEAPSFTLPSSQ
                     GKLVDYYKDYYGKHNLVMTFFPAAFTPV"
     gene            complement(1639..1884)
                     /locus_tag="VEL68_06340"
     CDS             complement(1639..1884)
                     /locus_tag="VEL68_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HXZ35617.1"
                     /translation="MSPKKSPFSRRPNPPGRPPSSFGQFEASELFCSRCRRAVPVRKR
                     LLLILPDGDKYEYLCAHCSNSLGTKMEFEEREVKILL"
     gene            complement(1881..2456)
                     /locus_tag="VEL68_06345"
     CDS             complement(1881..2456)
                     /locus_tag="VEL68_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ35618.1"
                     /translation="MADKRYAMLIDLRRCIGCHTCSLACKAEYEVPLGCWRTWVKQIE
                     KGRYPYVRKAFLPIVCNHCERAICTTVCPVKATYRRPEDGIVMINPHRCIGCRYCMAA
                     CPYNVRFVSPRYNIVEKCDFCQRRLDQGQRPACVEACPTQAMAFGDLNDPDGEITRLV
                     STSPVQVIKPEMGTYPQAFYIDMDVDIVGAK"
     gene            complement(2709..3923)
                     /locus_tag="VEL68_06350"
     CDS             complement(2709..3923)
                     /locus_tag="VEL68_06350"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35619.1"
                     /translation="MNSRIHWAWLVLAASFATVFIHYNIRLGYGILMPEMIQSLQITK
                     AQAGTIASSFYLAYSIFAPIMGFMADRVNARILLVVFPAIFSVGTFFMGKPQTLFQAC
                     LSFFVVGVGASAMWTPVVTLVQRWFESRRRGMALGLLSFSYAIGYGIMGIVMPSLVAQ
                     YGWRACWSLLAAVAILLVPLNGILIRNKPQDLHLKPWGEGSETTPQPPAKHPLSNIPF
                     RALVRLPSLWLTGISYCFIAFSAYGVNLFIVTYVNLELKYPFAQAARLASAIAFSGIP
                     GALLLPILSDFIGRKRCLVLICSGMAVSILLIIWAGSTWSALMAAVCVYGVFYASVWP
                     MYAATAADFFPREATGTVLGFWTIFYGFSLVLAPVAGGYIADATGTFHYSFLAGAISG
                     FLAMLLLLPLRK"
     gene            complement(3933..5246)
                     /locus_tag="VEL68_06355"
     CDS             complement(3933..5246)
                     /locus_tag="VEL68_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(A) polymerase"
                     /protein_id="HXZ35620.1"
                     /translation="MEKNAVPYNPMEQPITPRILSRSEHPISRKDIDPDALGVLYRLH
                     HHGFAAYLVGGCVRDLLLGKTPKDFDVATDAHPRQVRDLFRNSRLIGRRFRLAHVYFP
                     EGKYIEVSTFRRHSEFDEADPQAHPHSDNTFGTPAEDALRRDITINGIFYNIGDFSLV
                     DYVGGLEDLRQGIIRCIGDPEEKFVQDPVRMIRVIRHAARTGFRIEEKTYRSLLSHVD
                     RVRLCSPARVRDEFLRELQEGSAKESVRLMIETGMLFALFPSFLAPFEDEKRREYFLN
                     SVETLDRLRLLEENFSYGLYLPLFLLPFLDFYCPMEEFPPGRKGQTDFHLKVREWLIH
                     TLGPFSFTRHSREAAAQLLAHQRTLREFGPHGRLPLRLVRRPEFSQALHLFQLASKAR
                     GEEPLKFSWPLEEKRFHRRKRKRWFRRKTRRQAGGALAPGEPPQG"
BASE COUNT         1406 a         1481 c         1528 g         1216 t
ORIGIN      
        1 ctgaaattcg taaagaggag aactagagaa aaccccaggg cgcacctgcc ggtgcgccct
       61 aagatacata atttcggaac gggtcgaatt tcaatctatt tcaatccgta gataaactct
      121 ctttcaacat aagccaggaa accggatcgt cagctctaca atcccgcctt ccttagctca
      181 tcctcaaaat tttcatagaa gtctttcttc agaaacttga agctctgtgc gaaatcctcg
      241 aagtagtcga aaggctcctg ggcatacgtt ccattcattg gcctccacat ggtgcagacg
      301 aaagagacaa tccgttcccc cttcttcaac aggtagattt tggacttggc cttttcatcg
      361 cggttgggat tttccccctc catgtaaata gccaaggcat ctcttccccc aagggtggtc
      421 ttttccggct ttgacacccg cagatcgatc ccgctgttga acaggaaccg ggtgcagtaa
      481 tacttggccc tgttctccag gtccggggaa cttccgtacg gctcttcatc atacatgaag
      541 gtggtctggc tggggaacgg gcctcgttcg atcagcagaa atcccagctc aaaatctccc
      601 ccttccagat tcttcatgag ggcccagttc tgtgggggcc gcttaaattc gtacttgtcc
      661 aggaaaagaa ccattccgcg gtcggtggga gtgaggacct tctgcttctc ggcgggttgc
      721 aggccagcgc agccgaagcc gaagcccgcc accatcacca ataagatggc ccgggttcca
      781 agaaatcttc tttctctttt tccttccata ttttcctcct taattatttc agtccccggg
      841 actctttttc cagtttcagg agtagatcca ggatttcctt attattcgga gatccgtcct
      901 tgaggtagcg aacgattccc tttttatcga tgaccacggt tcgtcggccg aaggtgttga
      961 tcgaaccatc cggcccaaaa ggaggctttt cggagtatgc cccataggcc ttggccataa
     1021 agccaagcac attcgaaatg atgggaaatt ccagcgccag gctttcagcg aaaaacttgt
     1081 tggtgatcgg gtcatccttg ctgacgccca ggacctgggc gtttaggcgc ttatagagat
     1141 agaggtctct attgtgcccg tccatctccc ccgttcaaac cggggtgaag gctgcgggga
     1201 agaaggtcat gaccaggtta tgcttcccgt agtagtcctt gtagtaatca accagtttcc
     1261 cctgggaaga gggaagggtg aaggaagggg cttcgtcccc gacctttaac tgcgcccagc
     1321 cgtatcccat acccaaaacc aggaatatgg gtacagcgat cagggtcata aacagaagtc
     1381 tctttcttgg cataaaacct cctttctttt ctcgttttga aaaatccttg gcccgagact
     1441 tatgtggaga ccccgcgaag gaatctcacc ttctcggtat ttattccatt ctgcaatttt
     1501 acagcaggtc aaagaggaaa caaatggggg gaattaggta tttttcgatg aaaaagactt
     1561 gattttagaa aaaacccccg gtcgcggcgg tcccccaggg atctccggat cagttttcag
     1621 gcgggagggt tgaactcatc agagcaggat cttcacctcc cgctcctcga actccatctt
     1681 ggttcccagg gaattagaac agtgggcgca gagatactcg tacttgtccc catccggcag
     1741 aatcaagagg agacgcttgc ggaccggaac cgccctccgg cagcgcgaac aaaaaagctc
     1801 gctggcttca aactggccga aggacgacgg gggtcttccg ggcggattcg gacgtctcga
     1861 aaaaggggat tttttcggac tcacttggct ccgacgatgt ccacatccat gtctatgtag
     1921 aaagcctggg gataggtgcc catttccggc ttaatgactt gcaccggaga ggtggagacc
     1981 agccgggtaa tttctccatc gggatcgtta aggtccccga aggccatcgc ctgggtaggg
     2041 caggcctcca cgcaggcggg gcgctgccct tggtcaagcc gccgctggca gaaatcgcat
     2101 ttttcgacga tgttgtaccg agggctgaca aagcgaacat tgtaaggaca ggcggccatg
     2161 cagtaccggc atccaataca ccggtgggga ttgatcatga cgatcccatc ctccggtcgc
     2221 cggtacgtgg ctttgaccgg gcaaacggtg gtgcaaatgg cccgctcgca atgattgcac
     2281 acgattggca aaaaggcttt ccgtacataa gggtagcgac ctttttcgat ctgcttgacc
     2341 caggtcctcc aacatcccag ggggacttca tactccgcct tacaagccaa agagcaagtg
     2401 tgacagccga tgcatcttct aagatcaatc aacatggcat accttttatc tgccattgtt
     2461 ttcccctttc aacataaatt cataacatag gtgggaaaaa aaagacaacc cctttcttat
     2521 ttttgccccc tccctcccag gccgggcaag gcttgaaatt caataggact accgcctact
     2581 tgccgaaaag tgaaggggga aaactttctt attttttcgg ccccagatca ggcaaaaaaa
     2641 ccacattgtt ctatttgctt atgtccaaat atacaatttc ccaggatggt ccagaggggc
     2701 catacctcct actttcttaa gggcagcaac aaaagcatcg ccaagaatcc gcttatggct
     2761 ccggccagaa aggagtaatg gaacgtaccg gttgcatccg cgatatatcc ccccgctacc
     2821 ggggctaaga ccagagaaaa cccgtagaag atagtccaga accccagtac cgttccagtc
     2881 gcttcccgcg ggaaaaaatc cgccgccgta gccgcataca tgggccagac cgaggcataa
     2941 aagaccccgt acacgcaaac cgcggccatc aaagcagacc aggtgcttcc tgcccaaatg
     3001 atcagaagga tgctgacggc cataccggag cagatgagaa ccaagcatct cttgcgcccg
     3061 ataaaatccg aaaggatggg gagaagaagg gccccgggaa ttccggaaaa ggcgatggcg
     3121 ctggccaacc gggcagcctg ggcgaaggga tacttcaatt ccaaattcac ataggtcacg
     3181 atgaacaaat tgaccccgta agcgctgaag gcgatgaaac aataggagat gccggtcaac
     3241 cagagactgg gcaatcgcac gagcgcccga aagggaatgt tgctcaaggg gtgtttcgcg
     3301 gggggttggg gggtcgtttc actcccttct ccccagggct tcagatgcaa atcctgtggt
     3361 ttgttgcgga ttaatatccc gttcaggggc acgagtaaga tggccacagc cgccaggagc
     3421 gaccaacagg cccgccaacc gtattgggcc accagcgacg gcataacgat tcccatgatt
     3481 ccgtatccga tggcatagct gaaggacagg aggcctaaag ccattccccg ccgacgggac
     3541 tcgaaccatc gctgaaccag ggttactacc ggcgtccaca tggccgaagc tcccactccc
     3601 accacgaaaa aagaaagaca ggcctgaaaa agggtctggg gcttgcccat gaagaaagtt
     3661 cccacggaaa agatggcggg aaaaacgacc agcaggatac gggcgtttac tcgatcggcc
     3721 atgaacccca tgatgggagc aaaaatgcta taagccaggt agaaagaact ggcgatggtc
     3781 cccgcctggg ccttggtgat ctggagagac tggatcattt ccggcatgag aatcccataa
     3841 cccagccgga tgttgtagtg aataaaaact gtggcgaagg aagcggcgag gactaaccaa
     3901 gcccagtgga tgcgggagtt catggggaac catcacccct gcggaggttc gccaggggca
     3961 agggcgcccc cggcttgtct tcttgttttc ctgcggaacc agcgctttct tttccggcgg
     4021 tgaaaccttt tttcttccag gggccaggaa aatttaagag gttcttcccc cctggccttg
     4081 ctggccaatt gaaaaagatg gagagcctga gagaattccg gccttcggac cagtcgaagc
     4141 gggagtcttc cgtgggggcc aaattcgcga agcgtgcgct ggtgagcgag gagctgggcc
     4201 gcggcttccc gggaatggcg ggtgaatgaa aatggcccca gggtgtggat cagccattcc
     4261 cgaaccttaa gatggaaatc ggtttgccct ttgcgccccg gggggaattc ttccatgggg
     4321 caatagaaat cgaggaaagg aaggaggaag aggggcaaat agaggccgta ggaaaaattc
     4381 tcctccaaga ggcgaagtcg atccaaagtc tctacggagt tcaggaaata ttctcggcgc
     4441 ttttcatcct caaaaggggc gagaaaagac ggaaatagag cgaagagcat tcccgtctcg
     4501 atcatcaagc gcacggattc tttggccgac ccttcctgca actcccggag aaattcgtcc
     4561 cggacccggg ccggagagca cagccggact cgatccacgt gcgagagcag agaccggtag
     4621 gtcttttcct cgatcctgaa gccggtccga gccgcatggc ggatgacccg gatcatccgt
     4681 accgggtcct ggacgaactt ttcttcggga tcgccgatgc agcggatgat tccttgccgg
     4741 aggtcttcca gccctcccac gtaatccacc agcgagaaat cgccgatatt gtagaagatg
     4801 ccgttgatgg tgatgtcccg ccgcaaagca tcttcagcgg gggtcccgaa agtgttgtcg
     4861 gagtgggggt gggcttgcgg gtccgcttca tcaaattccg aatgccgacg gaaggtggag
     4921 acctcgatgt atttcccctc ggggaaataa acatgggcca ggcggaagcg gcggccgatg
     4981 aggcgggaat tgcggaagag gtcccgcacc tgccggggat gggcatccgt agccacatcg
     5041 aagtctttcg gtgtctttcc caggagaaga tcgcgcacgc atcctcctac caggtaggcg
     5101 gcaaaaccat ggtggtggag gcggtaaagg acccctaacg catccggatc aatgtccttg
     5161 cgggagatgg ggtgctccga gcgggagaga atacgcgggg tgatgggttg ttccatgggg
     5221 ttgtagggta cggcgttttt ttccacggcc tcctgaggac gatccagaca tcggaagtct
     5281 ttgcagatga aaggggtttc cccctttttt ttaaatttct aatgaaaaag gggcatttgt
     5341 caaggaaaca atcgaatgga gaagagggag tgatgagatg aatggaaaag aaaggaaggg
     5401 gtatccgaaa tcggcaattg aaaatcctat gtaaaaaagc aataccgccc ccgccaggga
     5461 gcgagtggag tgccgcctgg ggggggaaat ccgcctaaga gaaaaaatcc cgccaaagtg
     5521 gaataattcc atcctcataa taagaatttc cagctcttga ccatccaacc atctccttgg
     5581 actatttgat taaagaagaa ttaacccata accatccgta atttttacct t
//
LOCUS       DASPRR010000163         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2136676, whole genome shotgun sequence.
ACCESSION   DASPRR010000163 DASPRR010000000
VERSION     DASPRR010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136676"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VEL68_06360"
     CDS             complement(<1..383)
                     /locus_tag="VEL68_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HXZ35621.1"
                     /translation="MISSKMVSAVTFLYLFCTVLYFIYLAFRAERLGKFATLAAWVSL
                     GIQTAAILLRWVESYRMGFGHAPLSNMYESLVFFSWCIALLYLLWERKLKSRVIGAFA
                     LPFAFAFNAYASLASGVSAKIDPLIP"
     gene            complement(380..1780)
                     /locus_tag="VEL68_06365"
     CDS             complement(380..1780)
                     /locus_tag="VEL68_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HXZ35622.1"
                     /translation="MSTTENEKGSLSSLWDSLSSVRLTIPLLIVLAVVSIFGTLIPQN
                     GTPEEYLRIYSHSTYKVLRILGFLDMYHAGWFIFLLALLSLNLMACSLRRWPSVHKFF
                     SQPEIKLENRQWKATAPGRRFSLPLSARDALGQIQSSLSRTFAQPRALQEEGLGYLFA
                     EKGKLSRLGFYCIHLSILVILLGAMIGSFYGFRGYVNITEGEAVDQVFLRNGQQVQPL
                     GFKVKLDQFTVSFYPTGAPQEFKSTVTILEGGQRVLTEPIRVNEPLTYKGISLYQSSY
                     GVASIEKAILDVVERSSRRGFTLSLQPGVRAEIPGTPNSLVLDRFIPDLQGMGPAFQG
                     RLLEGDRPVENLVILQSRPEFFPHKPGRYEFRVREIQPKYYSGLQVNKDPGVWVVWAG
                     CLLMMVGFYMTFFMSHRRVWVRITEKKGETQVEMAGSSHRNRSEFEKEMDRIGQAAKE
                     AVSSKKKKKGAAGGPS"
     gene            complement(1854..2198)
                     /locus_tag="VEL68_06370"
     CDS             complement(1854..2198)
                     /locus_tag="VEL68_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HXZ35623.1"
                     /translation="MKNQTWKVAVITVSIVCFALLGIAFAQAKKVGGGDIKFTPKGAD
                     PVVFSHENHVTTQKMKCTDCHTKIFPMKKQDLKMTKEGHGQDKHCGVCHNGTKAFSQN
                     KDTECVKCHKKA"
     gene            2798..>3037
                     /locus_tag="VEL68_06375"
     CDS             2798..>3037
                     /locus_tag="VEL68_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ35624.1"
                     /translation="MIREKEVKSILSKSGIPGVKYCLNPYVGCAHACKYCYATFMKRF
                     TGHTEPWGTFVDVKRNAPEVLRRQLKRITRGYVMIS"
BASE COUNT          760 a          805 c          794 g          678 t
ORIGIN      
        1 gggattaggg gatcgatttt ggcggagacc cccgaggcca aggaagcata ggcgttaaat
       61 gcaaaggcga aagggagagc aaaggccccg atcacccggg atttcagttt ccgttcccag
      121 agcaggtaga gaagggcgat acaccaggaa aaaaaaacca gggactcgta catattggag
      181 agaggagcat ggccaaagcc catccggtaa gattccaccc agcgcaggag gatagccgcg
      241 gtctggatcc ccaggctgac ccaagctgcc agggtggcga attttcccag ccgctctgcc
      301 cggaaagcca ggtagatgaa gtaaagcacc gtgcagaaga ggtaaagaaa ggtcacggcg
      361 ctgaccattt tagagctgat catgacgggc ctcctgcagc acccttcttc ttttttttgg
      421 aggaaaccgc ctctttcgca gcctgcccga ttcgatccat ctctttttcg aattcagacc
      481 gattccggtg gctggagccg gccatctcca cttgcgtttc tccctttttc tccgttattc
      541 taacccaaac ccgccgatgg gacatgaaga aggtcatata gaagcccacc atcatgagca
      601 agcaacccgc ccaaaccacc cagactcccg ggtctttgtt tacctggagc ccggagtaat
      661 atttgggctg aatctccctg acccggaact cataccgccc gggcttgtgg ggaaaaaact
      721 cggggcgact ctgaaggatc accaaattct ccacgggccg gtccccctcc agcagtctgc
      781 cctggaaggc cggtcccatg ccctgaaggt cggggatgaa gcggtcgagc accagggagt
      841 tgggagtccc cgggatctcc gcccgtaccc ccggctgaag ggaaagagtg aaccctctcc
      901 tggaagaacg ctccaccaca tcgagaatgg ccttttctat tgaagccact ccgtagctgg
      961 attgatacag ggaaatcccc ttgtaggtca gaggttcatt gactcgaatg ggctccgtca
     1021 gaaccctctg gccgccttcc aggatcgtca ccgtactttt gaactcctga ggcgctcctg
     1081 tggggtagaa ggaaacggtg aactgatcca gcttgacctt aaaacccaaa ggctggacct
     1141 gctgcccgtt tcgcagaaac acctggtcca cggcctcgcc ttcggtgatg ttcacgtatc
     1201 ccctaaaccc atagaaggac ccgatcatgg ctcccagcag aattaccaga atgctgagat
     1261 ggatgcaata gaatcccagc cgggacaatt ttcccttttc cgcaaaaagg tatcccagcc
     1321 cttcctcctg caaagcccgg ggctgcgcaa aagttcggga aagactactc tgaatttggc
     1381 ccagcgcgtc ccgggccgag agaggaaggg aaaaccttct ccccggcgct gtcgccttcc
     1441 attgccgatt ttcaagcttt atttccggct gggagaaaaa cttatggacc gagggccacc
     1501 ttctcaggga gcaggccatc aggttcaggc tgagaagagc caggagaaag atgaaccacc
     1561 cggcgtggta catgtctagg aatccgagga ttcgcaggac cttgtaggtg gaatgactat
     1621 aaatgcggag gtattcctct ggcgtgccat tttgggggat cagggttcca aagatcgaga
     1681 ccacggccag gacaatcaaa aggggaatcg tcaggcgaac cgaagagagg gaatcccaga
     1741 gggaggagag agatcctttt tcattttccg tggtggacaa cggctacctc ataaaaaaac
     1801 tggcgttgag cttcggccca acgccagcaa gatggaccca atttgccgat gaatcacgcc
     1861 tttttatggc atttaacgca ctcggtatct ttgttctggc tgaaggcctt agtcccgtta
     1921 tggcagaccc cgcagtgctt atcctgcccg tgaccctcct tggtcatttt taggtcttgt
     1981 ttcttcatgg ggaaaatctt ggtatggcaa tcggtgcatt tcattttttg cgttgtcacg
     2041 tggttttcat gactgaaaac gactgggtcc gctcccttgg gagtaaattt aatatctcct
     2101 cccccgacct tcttcgcttg ggcaaaagcg atgcccagga gggcaaagca gacgatgctg
     2161 acggtgataa ctgcaacctt ccaagtttga tttttcaatt ttcatcccct ttccaagaag
     2221 agttgataat gattcaaatt ttgccacttc atgacccttt ctaacaagaa atagtttatc
     2281 ctcagaaaga aattcccgtc aaggagaaaa attggcttgc cccccagttc ttaaaaaaag
     2341 tccttcgaaa ataagatctc tagaaaggag gggcgatctc atgggattca gggcggagaa
     2401 atttggatca tcatcctgag gcggtggttc ttccatggga tcgggcctgt cctggggggg
     2461 ttgaaacgga aaaatgcttt ggctctcttt tctgcttgga ggtatcccct ctcaggcctc
     2521 tccctgcgga acatgggaga agtttcatgg aaggataagc acccgccgag cctgatgatc
     2581 caaattcctc caccctgaac cccgacacgc acgaaaaact gggggtggac aaggaaaaat
     2641 tgggtagata gcctcccccc atcccggccc ttccagccag caataatatc ggcaaggatt
     2701 ctacgccgat ttatgctaat ttaagagaaa caagcggtcc ccctatcgcc taaggaaaaa
     2761 tccgggatga acgtgggaac cacccacggg agaattcatg attcgggaaa aggaagtcaa
     2821 gtctattctt tccaaatcgg gtattcctgg agttaaatac tgtctgaacc cttatgtggg
     2881 ctgtgcccat gcctgcaagt actgttatgc caccttcatg aagagattca ccgggcatac
     2941 cgagccctgg ggcaccttcg tggacgtgaa aaggaatgcc ccggaggttc ttcggaggca
     3001 actaaaaaga ataactaggg gatatgtgat gatcagc
//
LOCUS       DASPRR010000164         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1167506, whole genome shotgun sequence.
ACCESSION   DASPRR010000164 DASPRR010000000
VERSION     DASPRR010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167506"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VEL68_06380"
     CDS             <1..1283
                     /locus_tag="VEL68_06380"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HXZ35625.1"
                     /translation="IDLSGAGDIYVIGAGKTVQRQAKALEDLLGNRLTAGAVTIKRGE
                     EVLLKKIEVTEGAHPVPDEKSVEGTRKIAEIARRAKKGDVVFALFSSGSSSLFALPLK
                     GYPLEEMRAVYKLAIQYGDQSIIWRVMKYFSAVNSGRIVMMIHPARVVNLLMSIKGYE
                     PWQGRIPEGGDWIPSWPPGPRRLADAAEEMKTEPWWEELPWSMRTALERHDPKCEVPS
                     LEDFRRVQASYWQPVNNRRMMEAARAKAEECGFHGVILGNWMMVESSDAAEVMIGIAR
                     ECLRHETPFKPPVALISGGEMTVPVGTATGIGGRNQEFVLTAAMRISEMGEKGIVIGS
                     VDSDGTDGPGTQLVSGPGSALRTLAGGVVDGKTLADALAMGIDLRMELKNHNSTPVLT
                     SLKSGLYTGNTGIVGGDLRVVLIPNSNFQIPNPK"
     gene            1459..2739
                     /locus_tag="VEL68_06385"
     CDS             1459..2739
                     /locus_tag="VEL68_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXZ35626.1"
                     /translation="MSSGTAIVSVTARQVYSDRGHPGIEATVKTENGAVGVAVCTAGI
                     SVGQHEVQFAYDGGTKWRGRGVQKAVDNVNQMIAPALKGMDASRQSEVDDAMLSLGGP
                     GAKLRLGGNATAAVSAAVLKAGAAGLGIPLYQHIGGVNACTLPVPGEISFMGSDRYGS
                     GKRSGGKPSYSFMAYGFDTFSDASYAIWDISTEWREALNRKFAIPKVSRTSHPVIQAG
                     VIRHDREIWDLMNETVHRLGYGGKVGFQVDVAAGTYYEKDRDRFVGLFSSEDKTRDDL
                     MKLYRQMVKDYPFVILEDPLDEDDYEGHAVLTRELGIQLVGDDLFTTNAERLEKGIQA
                     GAANTVLLKVNQIGTITEAFEMVRMAYGHGYGVMPCDSRGEGADIGDYSVGLNCGTIR
                     ESATGPRGNRLLQIEAELGRRAQFLGKKGFKGKL"
     gene            complement(2962..3576)
                     /locus_tag="VEL68_06390"
     CDS             complement(2962..3576)
                     /locus_tag="VEL68_06390"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXZ35627.1"
                     /translation="MKRRCEWSGSDPLYLEYHDREWGVPLHDDRRLFEMLILEGAQAG
                     LSWITILKKREAFREAFDSFDPSRVARFDGRKVRELLSNRGIIRNRLKIQSAVQNARA
                     FLEIREEFGTFDRYVWQFVGNSPIHNRWKKLQEIPARTKESDALSRDLKKRGFKFVGS
                     TICYAFMQAVGMLNDHVIDCFRYGEIKSAATGRGGSRRVDPSGA"
BASE COUNT          824 a          899 c         1118 g          745 t
ORIGIN      
        1 tcatcgacct ttccggggca ggagatattt atgtgatcgg tgccggcaaa acggtgcaaa
       61 ggcaagccaa ggcgctggag gatttgctgg ggaatagact caccgcaggg gccgttacca
      121 tcaaaagggg tgaagaggtc cttctcaaga aaatcgaggt aaccgagggg gctcacccgg
      181 tccctgatga aaaatcggtg gaggggaccc gtaagattgc cgagattgcg cgccgagcaa
      241 agaagggaga cgtagttttt gccctctttt ccagcgggtc ctcttctctg tttgcccttc
      301 cgctgaaagg ctaccccctg gaagagatga gggctgttta caagctggcg atccaatacg
      361 gcgaccagag catcatctgg agggtgatga agtatttctc ggcggtgaac agcggaagaa
      421 ttgtgatgat gattcatccg gcgcgggtcg tcaatcttct catgtcaatc aaggggtatg
      481 agccctggca gggaagaatc cccgaggggg gtgattggat tccgtcctgg ccgccggggc
      541 cccggcggct tgcggatgct gcggaggaaa tgaaaaccga gccctggtgg gaggagctgc
      601 cttggtccat gcggacggcc ctggagcgcc atgacccaaa gtgcgaggtc ccgagcctcg
      661 aagatttccg gcgggtccag gccagttact ggcagccggt caataaccgg cggatgatgg
      721 aagcggccag ggcgaaagcg gaggagtgcg gattccacgg agtcatttta ggaaactgga
      781 tgatggtcga gagttcagat gcagcggaag tcatgatcgg aattgctcgg gaatgcctgc
      841 gtcacgaaac ccctttcaag cccccggtag ccctgatttc cggaggggaa atgaccgtac
      901 ctgtgggaac agccacgggg atcgggggac ggaaccagga attcgtcctg acggctgcga
      961 tgaggatttc ggaaatgggc gagaagggaa ttgtgatcgg ttcggtggac tcggacggaa
     1021 ccgacggccc gggaacccag cttgtgtcgg gacccggctc tgccttacga actctggccg
     1081 ggggagtggt cgatgggaag accctggccg atgcattggc gatgggcatc gacctgcgga
     1141 tggaattgaa gaaccacaat tccactccgg tgttgacgag cctgaaaagc ggactttata
     1201 cgggcaacac gggcattgtg gggggagacc taagggtcgt actgattcca aattcgaatt
     1261 tccaaatccc aaatcccaaa taagctccga atcctaaaat ccaaaatcga aactcgcttg
     1321 catgtttggt tgattccatt tttgatcatt gatatttatt tggaatttgg tgcttgggat
     1381 ttggtatttt catagcagtg catgcagggt gacatctgtg catcccaaga acagagcaca
     1441 gagagggagg aggcgcttgt gagttcagga acggccatcg tatcggttac ggcgcggcag
     1501 gtttattcgg atcgggggca tcccggcatc gaggccacgg tgaagaccga gaacggagcc
     1561 gtgggagttg ccgtctgcac cgctggaatc tcggtgggtc agcatgaggt acagtttgcc
     1621 tacgacggcg gaaccaagtg gcgggggcgc ggcgtgcaaa aagcagtgga caacgtgaac
     1681 cagatgattg ccccggcgct caaaggtatg gatgcttcga ggcagtcaga ggttgatgat
     1741 gccatgctca gcctcggagg tcccggagcc aagctgcggc tgggtggaaa cgcaacagcc
     1801 gccgtgtcgg ccgcggtgct gaaggccgga gcagccggcc tgggaatccc cctgtaccag
     1861 cacatcggag gcgtaaacgc atgcaccctg ccagttccgg gtgaaatcag cttcatgggc
     1921 agcgaccggt acgggagcgg gaaacgctcc gggggaaaac ccagctattc cttcatggcc
     1981 tatgggttcg atactttttc agatgcttct tatgccatct gggacatctc caccgaatgg
     2041 agggaggcgc tcaaccggaa attcgcaatc cccaaggtct ccaggacgag tcatccggtc
     2101 atccaggccg gcgtgatccg gcacgaccgt gaaatctggg atctcatgaa cgaaaccgtc
     2161 caccggctag gctacggggg aaaggtagga ttccaggtgg acgtggctgc gggaacgtac
     2221 tatgagaaag accgggatcg ctttgtgggt ctcttttcct ccgaggacaa gacccgcgat
     2281 gacctgatga aactttaccg gcaaatggtg aaagattatc ccttcgtcat cctggaggac
     2341 cccctggacg aggatgatta tgagggccat gcggtattaa cccgagagct tggaattcag
     2401 cttgtgggag acgacctgtt taccaccaac gccgaacgac tggagaaggg gattcaagcg
     2461 ggagcggcga acacggtgct tctgaaggtg aaccaaatcg ggacgatcac cgaggccttc
     2521 gagatggtcc ggatggccta tgggcatggc tatggagtca tgccctgcga cagcaggggc
     2581 gagggggctg acattgggga ttactcggtg gggttgaact gcgggaccat ccgggagagc
     2641 gctaccggac cccgaggcaa ccgacttttg cagattgaag ccgaactggg cagacgggcc
     2701 cagttcctgg gaaagaaggg attcaaagga aagttgtaag ggcgattcac gtcggagact
     2761 cgacctaaac gttgcataaa atttttgagg agtagggtcg tcattcccgc gaaagcggga
     2821 atccagtaag aaatcctgga ttccgggtca agcccggaat gacggatgtt aaagttctcc
     2881 gaaagtgata gccaggaaca gcatgagaat ccggatgaaa tgcaatccta tcaacagttc
     2941 tcatcatgcg ggagcatgaa tttatgcacc tgatgggtcg actctacgag aaccgcccct
     3001 acccgttgct gcacttttta tttctccata ccgaaagcag tcgatgacat gatcattcag
     3061 catccccacc gcctgcatga aggcgtagca gatcgttgag cccacgaatt taaatcccct
     3121 ctttttcaaa tccctgctca aggcgtccga ctctttggtt cgggcgggga tttcctgcag
     3181 cttcttccat cgattgtgga tgggactgtt tcccacgaat tgccagacgt agcggtcgaa
     3241 ggttccgaac tcttcccgga tttccaggaa ggcccgggca ttctgaacag cggactggat
     3301 tttcagtcgg ttgcggatga tcccccggtt gctgagcaat tcgcggactt ttcttccatc
     3361 gaaccgggcg acgcgtgaag ggtcgaaaga atcgaaggct tcccgaaaag cctccctctt
     3421 ttttagaatg gtaatccagc tcaagcctgc ctgggccccc tccaggatga gcatttcgaa
     3481 gaggcggcgg tcatcgtgga gtgggacccc ccattcccgg tcatggtatt ctaggtaaag
     3541 ggggtccgat ccgctccatt cacaacgcct tttcatgcgc tttctc
//
LOCUS       DASPRR010000165         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1007161, whole genome shotgun sequence.
ACCESSION   DASPRR010000165 DASPRR010000000
VERSION     DASPRR010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007161"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VEL68_06395"
     CDS             <1..998
                     /locus_tag="VEL68_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HXZ35628.1"
                     /translation="FFVHNRVQSIASMERFLRRLVPEARLAIAHGQMAERDLERVMLA
                     FVKKEVDLLLTTTIIESGLDFPAANTIIINRADKLGLAQMYQLRGRVGRAKERAYAYL
                     LVPGLAAMGSDARKRLEALQEVTELGSGLKLAMHDLEIRGAGALLGDAQSGHIAAVGY
                     DMYLQILEQAIQELKGEEVVEEVEPEIDLPIPALIPADYVEDINQRLVFYRRFSSGRS
                     QEAVEEIVEELRDRYGTIPPVLENLVEVMNLRLLMKKAGVKRVSTERERVVLTFDLHS
                     PVEPQRLVEVVAREGEKMEFTPDQRLKVRPAQKGGRGMLTETRKILMEILENGSI"
     gene            1053..2042
                     /locus_tag="VEL68_06400"
     CDS             1053..2042
                     /locus_tag="VEL68_06400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXZ35629.1"
                     /translation="MAFRFRLLRIQLVPCLLAVSFLLFPGGTRGEILERIVAVVNNDV
                     ITLSEWEEASKPLIEQVQQSSLPEEREEKEKKARRAVLDQLIENKLLEQEIKKRKVEV
                     SERDIDAAIEQIKQQNRVNEDELKAILAKEGISLSTYRRRLHDEIGKMRLISREIKSK
                     ILIKEEDLRKNYQDNLARFTDPAEVKVQQIFFVVPQWASEEQAAAIRKEALSILERAR
                     RGEDFAELAGKYSQAPEAREGGVLGFFKHKELRPELEEVAFRLQPGEVSGLIKSPEGL
                     HILRVLERKGGEPKPFAEVQNQIRDQMMQEETERQFQEWIKALKAKAYIEIRL"
     gene            2123..>3025
                     /gene="pdxA"
                     /locus_tag="VEL68_06405"
     CDS             2123..>3025
                     /gene="pdxA"
                     /locus_tag="VEL68_06405"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXZ35630.1"
                     /translation="MNSAKKDPKPKIAVTMGDPRGIGPEIVAKAFARAAPHDFCVPLV
                     LGDVAVLSRTVQRLNLGLRVAEVEEEGLQDIPAGVLPVLPVSTLSSEKEPGEIPPGES
                     SRASFAYVERAGRMAVEGKVEAMVTAPVSKEAIHRAGIAFRGHTEYLAEISGTRDFAM
                     MLAGDRLKVTLVTTHVPLETVAKLLTEEKIFSVIEITARGLQEYFELRSPRMAVAALN
                     PHAGEGGLFGNEEGIISRAVQRARDRRLAVSGPLPADSLFHRVQMGEFDAVVCMYHDQ
                     GLIPLKLLHFDTAVNVTLGLPFVRT"
BASE COUNT          716 a          726 c          963 g          620 t
ORIGIN      
        1 tcttcttcgt gcacaaccga gtgcaatcga ttgcctccat ggaaagattc ctgaggagat
       61 tggttcccga agcccgctta gccatcgccc acggacagat ggccgagcgg gacctggagc
      121 gggtgatgct ggcttttgtc aaaaaagagg tggacctgct gctgaccacc acgatcattg
      181 agtccggact ggactttccc gcggctaata cgattatcat caaccgggcg gataagctgg
      241 ggctggccca gatgtaccag ctccggggcc gggtgggacg ggccaaggag agggcctacg
      301 catatcttct ggtacccggc cttgccgcga tggggtccga tgcccgcaag agactggaag
      361 ctttgcagga ggttacggaa ttgggttccg gtctgaagtt ggccatgcat gacctggaga
      421 tccggggtgc cggggcgctt ctgggagatg cccaatccgg gcacatcgcc gcggtgggat
      481 atgacatgta cctgcaaatt ttggagcagg cgatccagga actgaaagga gaagaggtgg
      541 tggaggaggt ggagccggag atcgatctgc ccatccccgc cctgatcccc gcggattatg
      601 tggaagacat caaccagcgg ctcgtctttt accggcgttt ttcctcgggc cggtctcagg
      661 aggcggtgga agagatcgtg gaggagctgc gagaccggta tgggaccatt ccccccgttt
      721 tagaaaacct ggttgaggtt atgaacctga ggttgttgat gaagaaggcg ggggtgaagc
      781 gtgtttctac ggagcgggaa agggtggttc ttaccttcga ccttcattcg ccggtggagc
      841 cgcaacggct ggtggaagtt gtggcccggg aaggtgaaaa gatggagttt acgccggatc
      901 aacgtttaaa ggttcggccc gcgcaaaagg gcgggagggg aatgctgaca gaaacgagaa
      961 aaatattgat ggagattcta gaaaatggta gcatttaaaa ttcaatccgg tttcgctgga
     1021 tgaaaattcc cgcatgggtt ttgtaatttg atatggcctt tcggttccgg ctcctccgga
     1081 ttcagctcgt cccttgtctc ctagcggtat cattcctcct tttccccggg ggcacccggg
     1141 gcgagatcct ggagcgaatc gtagccgtgg taaataatga tgtgatcacc ctctcggaat
     1201 gggaggaagc gagcaaaccg ctgatcgagc aggtgcagca gtcgagcctc cccgaggaga
     1261 gagaggagaa agaaaaaaaa gccaggcggg cggtcctgga ccagttgatc gagaataagc
     1321 ttctggagca ggagatcaag aaaagaaaag ttgaggtttc agagcgggat attgatgcgg
     1381 cgattgaaca aattaagcag caaaatcggg tcaacgagga tgaactgaag gcgattctgg
     1441 ccaaagaggg aatcagctta tccacctacc ggcggcggct ccacgatgaa attggaaaaa
     1501 tgcgcctgat cagccgggag attaaatcaa agatcctcat caaggaagaa gacctgagaa
     1561 agaattatca ggataacctg gcaagattca cggacccggc ggaagtaaag gtacagcaaa
     1621 ttttcttcgt tgttccgcag tgggcctcgg aggaacaagc cgcggccatc cgcaaggaag
     1681 ctctatccat tctcgaaagg gctcgccgcg gggaagattt tgcggaactg gccggaaaat
     1741 attcgcaggc gccggaagcc cgtgagggcg gagtcctggg ttttttcaaa cataaggagt
     1801 tgagaccgga gctggaggaa gtggccttta gactacagcc cggcgaagtg agcgggctca
     1861 tcaagagtcc ggaagggctt catatcctgc gggttctgga acggaagggg ggggagccga
     1921 aaccgttcgc cgaagtccag aaccagatcc gggatcaaat gatgcaggag gaaaccgaga
     1981 ggcaattcca agagtggatt aaagccttga aagcgaaagc ctacattgaa ataagacttt
     2041 aaaaaaaaca gtgtcaggat gagtgtccgt gtcagcaacg agaaaatact gaccctggat
     2101 accgacgcta ttttccccac tgatgaattc tgccaagaaa gatcccaaac ctaagattgc
     2161 tgtcaccatg ggagatcccc gggggattgg gcccgaaatt gtcgccaagg cctttgccag
     2221 agcggctccg catgattttt gtgtccctct ggttctcgga gatgtggcgg ttctctcccg
     2281 gaccgtccaa agattgaact tggggctgag ggtagcagag gtggaggaag aaggacttca
     2341 ggacattccg gccggagtgc tgccggtcct tccggtgtca accttatctt cggagaaaga
     2401 accgggagaa attcccccgg gggaaagttc tcgcgcttcc ttcgcgtatg tcgagcgggc
     2461 ggggagaatg gccgtggaag gaaaggtgga ggccatggtg acggcgcccg taagcaagga
     2521 ggccattcat agggccggga ttgcctttcg gggccacacc gagtacctgg cggaaatttc
     2581 gggtacgcgg gatttcgcga tgatgctcgc cggagaccgg ttgaaagtga ccctggtaac
     2641 gacgcatgtc cccctggaga ctgttgccaa acttcttacg gaggagaaaa ttttttcggt
     2701 tatcgagatc acggccagag gattgcagga atactttgag ctacggtcgc cgcgcatggc
     2761 cgtggcggcc ttgaatccgc atgccgggga ggggggactt ttcgggaatg aagaagggat
     2821 catttcccgg gctgtccaac gggcccggga ccggcggctc gctgtttccg gccccttgcc
     2881 ggcggactcg ctgttccatc gcgtccagat gggggaattc gatgcggtgg tctgcatgta
     2941 ccacgaccag gggctgattc ctttgaaact cctccatttc gacaccgcgg tgaacgtaac
     3001 tctcggcctt ccctttgtcc gcacc
//
LOCUS       DASPRR010000166         7110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1977531, whole genome shotgun sequence.
ACCESSION   DASPRR010000166 DASPRR010000000
VERSION     DASPRR010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977531"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="meaB"
                     /locus_tag="VEL68_06410"
     CDS             complement(<1..925)
                     /gene="meaB"
                     /locus_tag="VEL68_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HXZ35631.1"
                     /translation="MPSTEELVREALSGSVRALAKLITLVENEMPEAMEALRQLYPRT
                     GNAYIIGITGPPGSGKSTLTDKITKELRKKDFTVGIIAVDPTSPFTGGALLGDRLRMQ
                     DITSDEGVFVRSMATRGTLGGLSRATADTIKVLDAFGKDFIVIETVGVGQDEVDIVKT
                     ADTTLLISVPGLGDDIQALKAGIMEIADIFVVNKADREGADRVVTELSLMLDLSPAKS
                     TWRPPVIKTVGTLGEGIQEVAEKILAHRKFLEEGDGLLKKRNNRARQEILTLIEKEVT
                     KYIYRMLKYDLTFDEVIEQVVARKKDPYSYVK"
     gene            complement(942..1628)
                     /locus_tag="VEL68_06415"
     CDS             complement(942..1628)
                     /locus_tag="VEL68_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXZ35632.1"
                     /translation="MDIIGQALKNGQKSLSEYDSKRVLKAYGFPVVKEKLANTRAGAV
                     KAAKEIGLPVVLKACSPAIAHKTEKKLIEVDLRTLKEVQGAYDAIVERVGDSPLDGVL
                     VQEMIKGSRELVIGMIRDAQFGPCVMFGLGGIFTEVLKDVSFRIAPLEKRDALEMAQE
                     IKGAPILGPFRGMPAVDMDLLTSMLINAGKLGLENDAVKEVDANPLILSGNKPVVVDA
                     LIVLEGGVKP"
     gene            complement(1721..2128)
                     /gene="gcvH"
                     /locus_tag="VEL68_06420"
     CDS             complement(1721..2128)
                     /gene="gcvH"
                     /locus_tag="VEL68_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXZ35633.1"
                     /translation="MDYPDDLYYNRDHLWVRVQGNRGTVGVTDHAQREMGEILFVDLP
                     EESSQVEKNDNFGSLESSKTVAELRCPLSGEIISINKDLEEEPSLVNDDPYGNGWLVV
                     LEMDDPGELEDMLNAADYEELLEQKDEEEEEEK"
     gene            complement(2191..2751)
                     /gene="efp"
                     /locus_tag="VEL68_06425"
     CDS             complement(2191..2751)
                     /gene="efp"
                     /locus_tag="VEL68_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXZ35634.1"
                     /translation="MYTTSDFRKGLKIEIGGEPYTIVDFQHVKPGKGGAFVRTRLKSL
                     TSGNVLDMTFRSGDKVDKPDLEEREMQFLYESNGEYHFMDTNNYEQLFLTGDHLGDSK
                     DFLKENLVIKAVFHNKRPIGIETPMFVELKVAQTEPGMRGDTATGATKPATLESGAVI
                     QVPLFVNEGDILRIDTRTREYITRVK"
     gene            complement(2863..3939)
                     /locus_tag="VEL68_06430"
     CDS             complement(2863..3939)
                     /locus_tag="VEL68_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXZ35635.1"
                     /translation="MNGAKKSLRSLLQRKKLDAILFSSLENIRFLCGFTGSDGALIYG
                     QEERVFLSDSRYEEQAKGEVRGAIFKRYQKKNEGIAQSLRALRVKRLGFEASALTWET
                     YQRLKEHLSRVTFIPLTAEISGLRACKEPPEIEKMRKAIQIASESFLDTLPRLKPGVH
                     ERTAGDFLEVRMKRRGGEAPAFPTIVASGPRAALPHGKASDKGMEKGETVVVDFGVRY
                     QGYHSDETKTLILGRPDARQKKIYDLVRKAQERAIRATRPGVNVRAIDRAAREVISRA
                     GYGKFFGHGTGHGIGLAVHEPPTLSPRGSGVVEAGMIFSVEPGIYLPGWGGVRLEDLV
                     LVTDRGCEVLTYLSKDLKDNILKG"
     gene            complement(4051..4800)
                     /locus_tag="VEL68_06435"
     CDS             complement(4051..4800)
                     /locus_tag="VEL68_06435"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ35636.1"
                     /translation="MEEEKTGEIEILSSDSSLFVQEAESLRLQKKYDQAIQTCQEGLK
                     KTPEALSGRLILGKCYLESGRVAEARKELEKVAQAVEECLSVYKILAQVYLEEKNVDK
                     ALEALRKTLLFPGAEEEPSKRVTPLEKGLLHRGSHPPFATPPAFHMDSPKAVETAEIK
                     LPPEEDPSAKVAIQTDTLAEIYLKQGHLDRALSVYQDILARDPQNAAARGKFESLKER
                     MVKKEREESRQKVQARLEKWLTVVSSRNPEL"
     gene            complement(4844..5701)
                     /locus_tag="VEL68_06440"
     CDS             complement(4844..5701)
                     /locus_tag="VEL68_06440"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HXZ35637.1"
                     /translation="MPAFPHRLGLHVSIAGGIEKAPDRARDLGCTAMQIFSRNPRGWY
                     PAPLPPESIRIFKEKITERAIDPVVVHTPYLLNLASGDQVLYRRSVEALSEDLRRAEL
                     LGARFVVTHLGSPGAGNPAEGHRQVIKALKEVFQARSSVMLLLENSAGAGRTVGSSLE
                     EMGKIIEEAGRDCRLGFCFDTCHGFAAGYDFRSEEQSRGLAKEIDRTVTLARLKVLHL
                     NDCEGPLGARRDRHQHIGKGEIGLLGFKSLLRQEALRKVPLILETPKDKPGDDRRNLS
                     RIRALLKSL"
     gene            5895..6182
                     /gene="gatC"
                     /locus_tag="VEL68_06445"
     CDS             5895..6182
                     /gene="gatC"
                     /locus_tag="VEL68_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXZ35638.1"
                     /translation="MKISGQEVEHVALLARLKFSQAEKERFTTQLNSILEYMDKLKEL
                     DTSQVEPTFHAVTRKNVFREDQVLPSSPQDLTLSNAPDGDRGFFRVPKIIE"
     gene            6234..>7110
                     /locus_tag="VEL68_06450"
     CDS             6234..>7110
                     /locus_tag="VEL68_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXZ35639.1"
                     /translation="MNLYEMTAHRLHDLLARKEITSRELTESVLKRVEEVEGRIHAYI
                     TLTPDMALEQAAAADLRIQKGEAEPLTGIPLAVKDVICTRGVRTTCGSHILENFIPPY
                     DATVTEKLKAAGAVFIGKTNMDEFAMGSSTENSFYGPSRNPWDLDRIPGGSSGGSAAA
                     VAADECIVSLGSDTGGSIRQPAACCGVVGMKPTYGRVSRYGLVAFASSLDQIGPFSKD
                     VRDTALLLNAISGHEPRDSTSVDLPVPDYTRALGGDVRGMVLGVPQETFGKGIDPEVA
                     GSVREAIEVLRKLGAK"
BASE COUNT         1412 a         2152 c         1831 g         1715 t
ORIGIN      
        1 tcttgacata ggaatagggg tctttcttcc gggccaccac ctgttcgatc acttcatcga
       61 aagtcaaatc atatttgagc attctataaa tgtatttggt gacctctttt tcgatgaggg
      121 ttagaatctc ctgccgtgcc cggttgttcc gtttcttgag taacccatcc ccttcctcca
      181 ggaacttgcg gtgggccaga attttttcgg caacttcttg aatcccctcg cccagagtcc
      241 cgacagtctt gatgaccgga ggcctccagg tgctcttggc cggactcagg tccagcatca
      301 ggctcaattc agtcaccact cggtcggctc cctcccggtc ggctttgttg accacgaaga
      361 tatcggcgat ctccatgatt cccgctttta gagcctggat gtcgtctccc agcccgggga
      421 cggagatcag gagggtggtg tccgcggtct ttacaatgtc cacctcatcc tgccccacgc
      481 ccacggtttc gatcacgatg aagtccttgc caaaggcgtc gaggactttg atcgtatccg
      541 ccgtggccct ggaaagcccg cccaaggtcc cccgcgtggc catggagcgg acgaagaccc
      601 cttcatcgct ggtgatatcc tgcatccgca ggcggtctcc caggagagcc ccacccgtaa
      661 agggacttgt ggggtccaca gcgatgatcc ccaccgtgaa gtccttcttc cggagttcct
      721 tggtaatctt gtccgtgagg gtgctcttac ccgaacccgg ggggccggta attcctatga
      781 tgtaggcgtt tccggtccgg ggatacagct gccgtagagc ctccattgct tcgggcattt
      841 cattttccac cagggtgatc agcttggcca gggcccttac cgacccggag agggcttcac
      901 ggaccagttc ctcagtactg ggcatctcgc attattccct gtcagggttt gaccccgccc
      961 tccagcacta tgagagcatc gaccaccacc ggtttgttcc ccgaaagaat caggggattg
     1021 gcgtccactt ctttcaccgc atcattctcc agccccagct tccccgcgtt gatcagcatg
     1081 gaagtcaaaa ggtccatatc cactgcgggc atcccccgga aaggccccag gatcggcgcc
     1141 cctttgatct cctgggccat ctccagggcg tcccgttttt ccagtggggc aattcggaag
     1201 gagacatctt tcaggacttc agtgaagatt ccccccaggc cgaacatcac acagggaccg
     1261 aattgagcgt cccggatcat gccgatcacc agctcccggg accccttgat catctcctgg
     1321 accaggaccc cgtccaaagg gctatcccca actctttcca cgatcgcgtc ataggctccc
     1381 tggacttcct tgagggtcct caggtctact tcgatcaact ttttctccgt cttgtgggcg
     1441 atcgctgggg aacaagcctt cagaaccacg gggagaccga tctccttggc tgctttcacc
     1501 gcgcccgccc gggtgttggc cagtttttcc ttcaccaccg gaaagccata agccttcagg
     1561 actcttttcg aatcgtactc ggaaagcgat ttttgcccat ttttcaacgc ttgcccaata
     1621 atatccattc ccacctccga ggggatctgt tttcattcct atccattcgt cccccccagt
     1681 ttctagcaac cttccccggt tttgtcaaga aattcatcct tcacttttct tcctcttctt
     1741 cttcgtcttt ctgctccagg agctcttcgt aatcagcggc attgagcata tcctccaatt
     1801 cgccggggtc gtccatctct aaaaccacga gccaaccgtt tccgtagggg tcgtcgttga
     1861 ccagggacgg ttcttcttcg aggtccttgt tgatggaaat gatttccccg ctgaggggac
     1921 agcgaagctc agcgaccgtc ttggaggatt ccagcgaccc gaagttgtca ttcttctcca
     1981 cctgggagct ctcttcggga aggtccacga agagaatctc tcccatctct ctttgggcgt
     2041 ggtccgttac cccaactgtc ccccggtttc cctgcacccg gacccataga tggtctcgat
     2101 tgtagtagag atcgtccgga taatccatgt aggcctcctt ctaggcggat ccaatccgat
     2161 tatcattttc cccccaaggg ggattctgac ttatttgacc cgggtgatgt attcgcgggt
     2221 ccgagtatca atcctgagga tatccccttc attgacaaaa agtggaacct ggatcaccgc
     2281 cccggactcc agggtagccg gtttggtggc accggtcgct gtgtccccgc gcataccggg
     2341 ctcggtttgg gctactttca attccacgaa catgggggtc tcaatcccga tcggccgttt
     2401 gttgtgaaag acagctttga tcaccaggtt ctctttgaga aagtctttgc tgtcccccag
     2461 gtgatccccg gtcaggaaaa gctgttcgta gttgttggtg tccatgaaat gatattcccc
     2521 gttgctctcg taaaggaact gcatctccct ttcctccagg tccggcttat ccaccttgtc
     2581 cccggagcga aaggtcatgt ccaggacatt tccgctggtg agactcttca gccgggtccg
     2641 gacgaaagct cctcccttgc ccggctttac atgctggaaa tcgacgatcg tgtagggttc
     2701 ccccccgatt tcaatcttca gccccttgcg aaaatccgat gtcgtataca ttggaaatac
     2761 ctcataaaaa atttaaggtt ataggttcag ggccatagga agaactggat ttttttgccc
     2821 ctacccatga cccataacct atgacccatg acctctcgcc gattaccctt ttagaatatt
     2881 atccttcaag tccttggaga gataggtcag aacttcgcag cctcggtcgg tcaccaagac
     2941 caggtcttcc aggcgaaccc cgccccatcc gggaagatag attcccggtt ccacgctgaa
     3001 gatcatcccc gcttccacca cgcccgaccc gcggggggaa agagtcggag gctcgtggac
     3061 cgccaaaccg attccgtgcc cggttccatg cccgaaaaat tttccgtatc ccgcccggga
     3121 aatgacctct cgggctgccc ggtcgatggc ccgcacgttg accccgggcc gggtggcccg
     3181 gatagccctc tcttgggcct tgcgcactag gtcgtagatt tttttttgtc gggcatcggg
     3241 cctgcccagg atcaacgtct tggtctcatc agaatgatat ccctggtagc ggaccccgaa
     3301 atcgacgacc actgtctccc ccttttccat tcccttgtcc gaagccttac catgggggag
     3361 ggcggcgcgg ggtccggagg ccacaatggt ggggaaggca ggggcctccc ctccccttcg
     3421 tttcatccgg acctccagaa agtcccccgc cgttctttca tggactcccg gtttcagccg
     3481 gggcaaggta tccaaaaaac tttccgaggc aatctgaatc gccttgcgca ttttttcgat
     3541 ctctgggggt tccttgcagg cccgtagacc cgagatttcg gcggtcagcg gtatgaacgt
     3601 cacccgggat agatgctcct ttaacctctg gtaagtctcc caggttaagg cggaggcttc
     3661 aaagcccagt cgcttcaccc tcaaagcacg gaggctctgg gcgattcctt cattcttttt
     3721 ttggtatcgc ttgaagatcg ctccccgcac ctcccccttg gcctgttctt cgtagcggga
     3781 atcggaaagg aaaactctct cttcctggcc gtagatcaga gctccgtcac tgcccgtaaa
     3841 cccgcacagg aatcgaatat tctccaggct cgagaaaagg atggcatcca gtttcttccg
     3901 ctgcagaagg gaccggagac tcttcttcgc cccattcatc tttcggcgcc tcctgagaaa
     3961 catcgatttc gggtctgttc tctaaacatt tccttttctc tattcccctt tccatcttct
     4021 tggttccacg agaggatggc agggaaggcc tcaaagctcc gggtttcggg aggagactac
     4081 ggtcagccac ttttccaaac gagcctggac cttctggcga ctttcctccc tttctttctt
     4141 taccattcgc tctttcaggg attcgaattt tccccgggcc gccgcattct gggggtcccg
     4201 ggccaggata tcctgataaa cggaaagggc tcggtctagg tgtccctgct tgaggtagat
     4261 ttccgccagg gtgtccgtct gaatcgccac cttcgccgag gggtcttcct caggcggcag
     4321 ctttatttcc gccgtctcga ccgccttggg agaatccata tggaacgctg gcggagtggc
     4381 gaagggggga tgggacccac gatgaagcag gcccttttcc aagggagtga ctcttttcga
     4441 gggttcttct tccgcgccgg ggaaaagcag ggtctttcga agtgcttcga gcgccttgtc
     4501 gacgttcttc tcttccagat agacttgggc gagtatcttg taaacggaaa ggcattcttc
     4561 gacggcttgg gcgacctttt ccagctcttt tcgagcctct gcgaccctgc cactttccag
     4621 atagcacttg cctaagatca gccttccaga aagggcctca ggggtcttct ttaaaccctc
     4681 ctgacaggtt tggatggcct ggtcatattt cttctgcagc ctaaggcttt cggcctcctg
     4741 gacgaaaagg ctcgagtcgg aacttaggat ttcaatctcg ccggtctttt cttcttccat
     4801 ttttcccctt ccttccggag ggattttgcc ctgccgccaa ggatcaaaga gattttaaca
     4861 aggcccggat gcgggagaga ttgcgtcgat cgtccccggg tttatctttg ggagtctcca
     4921 gaatcaaggg cactttccgc agggcctcct ggcggaggag gcttttgaac ccgagaagcc
     4981 cgatttctcc cttcccgatg tgttggtgac ggtcccggcg ggcccccagg gggccttcac
     5041 aatcgttcag gtgcaaaacc ttcagacgcg ccagggtaac cgtccgatcg atttctttcg
     5101 ccaacccccg gctctgttct tcagagcgaa aatcgtaccc cgccgcaaac ccatgacagg
     5161 tatcgaaaca aaagccgagt cgacaatccc tccccgcctc ctcgatgatt ttccccatct
     5221 cttccagaga ggaccccacg gtccgcccgg cccccgcgct gttttccagg agaagcatga
     5281 ccgaggatcg agcttggaac acttctttca gagccttaat gacctgcctg tgcccctcgg
     5341 ccggatttcc tgcccctgga cttcccaggt gggtgaccac aaaacgggcc cccaagagtt
     5401 ccgcccgcct caggtcttcc gacagcgctt cgaccgacct tcggtaaagc acctggtctc
     5461 ctgaggccag gttaagaaga taaggcgtat gaaccacaac cggatcgatg gccctttcgg
     5521 ttatcttctc cttgaatatc cggatggact cagggggcaa aggcgcagga taccatccgc
     5581 ggggattgcg ggagaaaatc tgcatggcgg tgcatcccaa atcccgcgcc cgatcgggtg
     5641 ccttttcaat ccctccagcg atggaaacgt gcaatcccag gcgatgggga aatgcaggca
     5701 ttccgtgcct tctacgaaca aaattaagta tgaatataaa taagcctttt cccccatgtc
     5761 aagggcaaaa attcaaaatc tttccttgcc cttttaaccc cttgccccgc gatccccatt
     5821 tcaggaaggg attcttgaaa aaggatacct ccctgtgtta ccattttttt aaaccttcgt
     5881 tgggggaccc caagatgaag atcagcggac aggaagtcga gcacgtggcc ctcttggctc
     5941 gcctgaagtt tagccaagcg gagaaggagc gatttaccac ccagttgaac agcattctgg
     6001 aatacatgga caagctgaaa gagttggata ccagccaggt ggaacccacc ttccatgcgg
     6061 ttacgcggaa gaatgtcttc cgggaagacc aggtccttcc ctcctcccct caggatctca
     6121 ccttgagcaa cgctccggac ggcgaccgcg ggtttttccg tgtccccaag attattgaat
     6181 gatttttttg atctggcgca caactcgatt tgcgggaaac tgaaggtacc gacatgaacc
     6241 tctatgaaat gaccgcccac cggcttcacg atctgctggc ccggaaggaa attacctcgc
     6301 gggagcttac cgaatccgtc ctgaaacggg tcgaggaagt ggaggggcgt atccatgcct
     6361 acatcaccct gactccggat atggccctgg agcaagcggc tgccgccgac ttgcggattc
     6421 aaaaagggga agcagaaccg ctgaccggca tccccctggc cgttaaagat gtcatctgca
     6481 cccggggggt gcgcaccacc tgtggctccc atatcctgga gaatttcatc cctccctacg
     6541 atgccacagt aaccgaaaag ctgaaagcag cgggagccgt ttttatcgga aagacgaaca
     6601 tggacgagtt cgccatgggg tcttccaccg agaattcctt ctatggaccc agccgaaatc
     6661 cgtgggacct ggaccggatt ccgggaggtt ccagtggagg atcggcggcg gcggtagcgg
     6721 ccgatgaatg catcgtctct ctggggtccg acacgggagg gtccattcgc cagcccgcgg
     6781 cctgttgcgg ggttgtgggg atgaaaccga cctacggaag ggtttcccgt tacggcctgg
     6841 tggcctttgc ctcctccctg gatcagatcg ggcccttcag caaggatgta cgcgataccg
     6901 cccttctgtt gaacgccatt tccggccatg aaccccggga ctccacctcc gtcgatctcc
     6961 cggttccgga ttacacccga gcgctcgggg gcgatgtgcg aggaatggtc ctgggagttc
     7021 cccaggagac ctttggcaaa ggaatcgacc ccgaagtggc cggttcagta agggaagcga
     7081 ttgaagtctt gagaaaattg ggcgccaaga
//
LOCUS       DASPRR010000167        10192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1167604, whole genome shotgun sequence.
ACCESSION   DASPRR010000167 DASPRR010000000
VERSION     DASPRR010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167604"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VEL68_06455"
     CDS             <1..1425
                     /locus_tag="VEL68_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXZ35640.1"
                     /translation="NQSLRDVSYGGGTFVAVGKGGTILSSPDGVIWTGCNSGTGYDLN
                     GVSYGGNTFVAVGEGGAILASSDGLTWAARDSGTHQGLNKITYGKDTFVAVGRNGTLL
                     ASPDGAVWTPQPSGTHGHLDGIAYGKDTFVAVGEAILTSSDGKAWTEKKAGASHRIFG
                     VAYGGGVFVGVADNGGILTSSDASEWTARDTGIHLTLFAIAHGKERFLASGERGILLL
                     SDPLPSPQISVSSTSLDFGSVKVGDSSFTNLTISNSGSANLIIRQMNFSGADTLDFNT
                     RNDNCTGVTLSQSQSCTVQIVFSPHSTGSKSATLSISSNDPDTPTQTVSLSGSSSGVS
                     AISGSTGSFCFISFSTFGSELGKYLDLLREFRDVFLMETKIGRTVVDFYYRHSPAMSQ
                     VLARHDFLRRAVAFGLVPLLAAIAYVTLYASPAEKAILFLLIAGGVFAGGRLIRRSGR
                     DWINRRSDASKLGGAGGAIAIWGP"
     gene            1426..1920
                     /locus_tag="VEL68_06460"
     CDS             1426..1920
                     /locus_tag="VEL68_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ35641.1"
                     /translation="MGDIQIVKDYIPGSIGRVAELHGKYYQKHWGFGLFFEAKVAAEL
                     AEFLSRYDERRDGFWTASLEGNVEGSITIDGLHAEGQGAHLRWFIVSDEMRGSGIGNR
                     LIKAAIDFCQKCHYQRVYLWTFEGLNAARHLYEKSGFKLVEQHRGTQWGAEVNEQRFL
                     LQLQ"
     gene            complement(2331..9935)
                     /locus_tag="VEL68_06465"
     CDS             complement(2331..9935)
                     /locus_tag="VEL68_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXZ35642.1"
                     /translation="MNPKVVFNPHWKEQIGELLQKNPGLRVVDDPEKLEYYRQDLNAD
                     LPPMIRDLMLKTKPDILFQPTTEKHIREILAFARAHQIPLTLRGAGTGGFGGAVPTSG
                     GILMDLGLMNQVQVDVQARQLNAGPGARFVEIDRELARHGLTLLSIPSGKGGTLTGWI
                     VTGGIGLGTFRHGPVAKQLLSIRVITPAGEVRELKADDPDLPYFVSTEGQMGVIVEAT
                     LRVGERPPAYFPFAIPFFDSKDAFSLAKDLAKQPNIAPDDVIIFHSELVRVLPIPSGD
                     GGSSHPQNLVLVGFAQAEPADRFRRYLTQRGIKPAEEKVAQFLWEERSLPLSIKPLGP
                     SLLAAEVVLPLDRVAAYQERVRDWGERLGVTFYPISHLIDPERVLLLVMIPSDYRKAI
                     FYIDLLLVPMMVRLAIQGYGGKPYGLGIWNTPFLQDLFPPREVKRLVQFKKKVDPTGL
                     LNPGKFFGVSGRFSSLQKVLFQSGVFNFELATIQWLLFRLFTFLPEKVLRRRLPVVSR
                     GMEEIANDILSCAQCGRCVARCPVYRATGDETFTARGKLLNVKRALRASKIELAKVLP
                     LYFCLHCGRCDEECQVNLKHRPLFDRLEQVLAESIPFPLQAVAEFIRQVEESPDFQRF
                     LDVVRTGFDQKIQEPRQTFPRYRVAIDEEHCLHCGTCVDACMYSVRKRDEGDPRKVVV
                     ADETLCRGCGACLERCPQVAEGLAATTVELHPRYLQMEDPYWSGEVISRIDLEATTGK
                     IPVSGTGQGDPHRGLGNDGIRFGHFHIVGPAQNLLYESSEDAIAVQLGKRPKYLTFRG
                     DEIETPSPRLVKLKTPLLLEVMPVDGGHGMLAALLEAAGQLGTRLTLRLEEVKRMANR
                     VRGKTGHILVRLSAQEAKVLLSAKTLPAKLKDHFFDWVEVEVDAEVIQRSHELRTLFP
                     EGTIFSAVVWVTPGDVDAELHPQTRLRQTLDSLFPSIFDLLCLTSEFHPDKGYYPTTD
                     AVPAVHRFLVEKKERHRFSILASGGIRSAADAQKTIQRGANGIKIDWPALFVADPMAW
                     QKFRRKEPLSIPEDASLLVKRLVNLIRVWNVQIIEVLGASGFKDIKKTVGEENRLLIF
                     DDLEERVYDIFHSPSRRERNHRTNRARMEREGPGRGWRYRQLKDRVQLADLPHRFYSL
                     CLKDSCQKIFDRDYVWPASLLETVGRMASGDSQTLLLGCVEERGNLGDGFDDMRIRFS
                     TDPDQVDEDSLEDISTSLHLADSLTLRTPLLGAGMSVGSIGPGTWRARVLATRALETQ
                     LDTGEGGYPTLYLMDSKWNPLDLTDSQTALLSRVLEERRLIPLSELLEQADRELATLP
                     DYPPIRKALAKYPSSLEVQFVPIVAPEDETYISTELKTGLFGVTQETIRKAPRVVIAY
                     SQGAKQGVGGHLLGRKVFGLVSRLRGVAEGTSLISPFPFHNCYSIEDVKAFIDAVRMI
                     NARTTICIKVSPSPDIEFIVSGLARIAKDNRMVIEVWLDGPRGGTGAAPDIIKGQMGM
                     HMEYAIPICHEKLMDDGLRDFVVFTGSGGFRTWADIIKGVAMGLDGVILGTADLVAIG
                     CVRDRNCESGCRSGISTLEPRMQRLRDIEMNTRQIINFRAILQAQVIRALGALGMKDI
                     RELRGRYDCVTWPLLEGRVGERLRRRAQIQEAGQPMLSEGPPAAREILPGPLAAPSDC
                     GVAAVVSNHFIPSHVMDLMLDRMANRGMDGVGIWKGGCYPDHLDHYALNLLIKGTLQP
                     QAEQEIQAQYPGLLPRELQRRAREKILAERRRLMGEILEKYLKVLEIEAFDGNMEKYR
                     ISYRRDLEGEEQDFRSFGGKDPGDMFRFFVRVRPEEIHRFIVEALVNDPVWPPKEMRY
                     REVTIGNYGQNRHFLQEAEDEYIYRLSRRITQENYAEVKEKRAAVMSCGKNSGCWKTD
                     GRLIPWELPEAPVNVIHRRLATGSVVDQMNAHPFAELHTALTHNGETTNYRTMVNRLA
                     QFNLVPLVQTDTAVASLKLHLLSQYLQYPFDALVEAFSPTTGWKLSQLPSEVRQRFER
                     VQEVELESAPDGPYQYLCGRIDPYRRVIERLDIIDPSLLRPNVAMLYDDGENFVSIIC
                     SEKQGCDAGMQEMHRLGLIRTPVPPMTFTVTTGMLSRVFYDPRGRITGHEVLDKFGQP
                     VPIPRGTFHVAEEEKRGRRTSWRFEWESDLPNFFRERLPRFSFSEFQAALRQVAKDFS
                     ICRALEEMTRIHDYMVGWDTGGKDRGALYYLLSEEVNHVLDGSDGDGDIHRITAENAD
                     RLQSPMAGEQILAVDARGFQPEGIDARKVLSCFLDHSHRIGWRRFIVYRAQGQRGIGM
                     GMGAGSTGGTQVDVFGSPGEYCGAFNMGVRLRVHNHAQNFTGMVMHSGILEIHGDVGK
                     VAGYSAKGGTFYILGNVVDRGWVCAVSDPRGPGLEVNIVGTGYEHLGQALMGGSILML
                     GLYRDAEAIWHRLPSPYQGNKILAGASAGEVIFYDPFGRLEEAQYGGCVVKPIDEKKW
                     KDILGRLMKLEEIFSLGFSRVGDHFVGHMDGGAETIAPEHFCWVLPTGVLEGYH"
BASE COUNT         2046 a         3014 c         2951 g         2181 t
ORIGIN      
        1 aatcaatccc tgagagatgt atcctacggc ggtggcacct tcgtcgccgt ggggaaaggg
       61 gggacgatcc tttcctctcc ggacggagtg atttggacgg ggtgcaactc ggggaccggt
      121 tatgatctca acggggttag ttatggaggg aacacttttg tggccgtggg agagggagga
      181 gccatccttg cctcttccga tggcttgacc tgggcggcga gggattccgg gacccatcag
      241 gggctcaata aaattaccta cggaaaggac acctttgttg ccgtggggag aaacggaacc
      301 cttcttgctt ctcccgatgg ggctgtttgg accccgcagc cctctggaac acacggtcac
      361 ctggatggaa ttgcttatgg gaaagatacc tttgtggcgg tgggcgaagc catccttacc
      421 tcttccgacg gaaaagcctg gaccgaaaag aaagccgggg caagccatcg catcttcggg
      481 gttgcctatg gcgggggggt ctttgtgggg gtggcggata acgggggcat tctcacttcc
      541 tccgacgcct ccgagtggac cgcgagagac accgggattc acctgaccct tttcgccatc
      601 gcccatggga aagaaagatt cttggcctcg ggagaaaggg gtatccttct cctgtccgac
      661 cccttgcctt ccccccaaat ttcggtttct tcaacctcgc ttgattttgg gtcggttaaa
      721 gtcggggact cgtcattcac gaacctgacg atttccaatt ccggatcggc caacctgatc
      781 atccggcaga tgaacttcag cggagccgac acgctggatt tcaacacccg gaacgacaac
      841 tgcacggggg tgaccctttc ccagtcccag agctgcaccg tgcagatcgt tttctccccc
      901 cattccaccg ggtccaaaag tgcaacccta tcgatctcct ccaacgatcc tgatacccct
      961 acccaaaccg tttccctgag cggtagcagc agcggggtta gcgccatttc tggttcgacg
     1021 ggtagttttt gcttcatctc cttttctacg ttcggctccg agctcggcaa atatctggac
     1081 ctactccgtg aatttcggga tgtctttttg atggagacaa aaatcggaag aacggtggtg
     1141 gatttctatt atcggcattc tccggcgatg agccaggtgc tggcccgcca cgatttcttg
     1201 agaagagccg tggcgtttgg attggtgccc ctcctggcag ccatcgccta tgtgaccctc
     1261 tatgcctcgc ccgcggaaaa agcgatcctt ttccttctga tagccggagg ggtgttcgcc
     1321 ggagggcgac tgattcgaag atcaggaagg gattggataa accgcagatc agatgcgagc
     1381 aaattaggtg gcgcaggcgg agctatcgcg atttggggtc cttgaatggg cgacattcaa
     1441 atcgtgaagg actatattcc tggctccatc gggcgagtgg ccgaacttca tgggaaatac
     1501 taccaaaagc attgggggtt tggcctgttc ttcgaggcga aggtggccgc cgagctggcg
     1561 gagttcctca gcagatatga tgaaaggcgg gatggcttct ggaccgcatc cctagaggga
     1621 aacgttgagg gttccattac aatcgacggg cttcacgccg aaggtcaggg agcccatctg
     1681 cggtggttca tcgtgtctga cgaaatgcgt ggaagcggca tcggcaatcg gctcatcaag
     1741 gctgcgatcg acttttgtca aaagtgccat tatcagcgcg tctacctttg gacgtttgag
     1801 ggccttaacg cggccagaca tctctacgag aagagcggat ttaagctcgt agagcaacat
     1861 aggggcacgc agtggggtgc ggaggtcaac gagcagcgat tcctgctaca gctccagtga
     1921 ttccacccgg cgctaaaccg gacgcgcttg acaccgcgcc gctcagctct gatcggtcct
     1981 tccgaccgca ggcggtcggg aggaacaggc ttccgactga ggtgcaacgc gttctgcgcc
     2041 tgcggcatcc cttcgtcatc tcttgccacc aggcctaggg ctagggaccg agtttcacct
     2101 ttaaagatga ccgtagctca gggcttcagc cctgatacct ggctctgatt tatgacctct
     2161 gctcatagaa aatatcaacg cttttgatgt gttccattaa tccatggacc aacccgcgat
     2221 ctttgttccc agcctagtgg gcgtccgatc caatagttct tcctgcggat ccatccatgg
     2281 tcattctttt tatttgggct ttgaatttcg gacatcagat gtaacgatta ttagtggtat
     2341 ccttcaagga cacccgtcgg caaaacccag cagaaatgtt caggggctat tgtctccgct
     2401 cctccgtcca tgtgccccac gaaatggtcc cccactcggg aaaatcccaa ggagaatatc
     2461 tcctcaagct tcatcaggcg gcccaggatg tctttccact tcttttcgtc gattggcttg
     2521 accacgcacc cgccatactg ggcctcttcg agacggccga agggatcgta aaaaatgacc
     2581 tcgccggccg aagccccggc gagaatctta ttcccctgat agggggaagg cagacgatgc
     2641 catatggcct cagcatcccg gtagaggcca agcataagga tcgaacctcc catcagggcc
     2701 tgccccaagt gttcgtatcc ggtcccgacg atgttgacct cgagccccgg tcctcgcgga
     2761 tcgctcacgg cacaaaccca cccccggtcg accacgttgc ccaagatgta aaatgtgccc
     2821 ccctttgccg aatatccggc taccttgccc acatccccgt ggatttccag aataccagaa
     2881 tgcatgacca taccggtgaa attctgagca tggttgtgga cccgcaggcg gacgcccatg
     2941 ttgaaggccc cgcaatactc cccggggctt ccgaagacat cgacctgggt acctccggtc
     3001 gaacccgccc ccatgcccat gccaatgccc cgctgcccct gggctcggta cacgatgaat
     3061 ctccgccacc ctatccggtg gctatggtcg aggaaacaac tcagaacctt tcgggcatcg
     3121 atgccctcgg gttggaatcc ccgggcgtca acggccaaaa tttgttcccc cgccatcggc
     3181 gactggagcc ggtccgcatt ttcggcggtt atgcggtgga tatcgccatc tccgtctgag
     3241 ccgtccagaa catggttaac ctcttcgctc aagagatagt atagggctcc tcgatccttt
     3301 ccgcccgtgt cccatcccac catataatca tgaatccggg tcatttcctc cagtgcccgg
     3361 cagatggaaa agtctttcgc aacctgtcgc agggccgcct ggaactcgct gaagctgaag
     3421 cggggaagtc tttcccggaa gaagttgggc aaatccgatt cccattcaaa tctccaactt
     3481 gtcctccgtc cccttttctc ttcctctgcg acgtggaagg tgccccgggg aattgggacg
     3541 ggctggccaa atttatccag aacctcatgc ccggtgattc tccccctggg atcataaaag
     3601 acccggctca acatccccgt cgtgaccgta aaggtcatcg gcggcaccgg ggtccggatg
     3661 agtcccagcc tgtgcatttc ctgcattcct gcgtcgcatc cctgcttctc cgagcagatg
     3721 atactcacga agttttcccc gtcgtcatag agcatggcca cgttgggacg aagcagggat
     3781 gggtcgatga tatccagccg ctcgatgacc cgccggtagg gatcgatgcg gccacagagg
     3841 tattggtaag ggccgtccgg cgcgctctcc agctccactt cttgcacgcg ctcaaagcgt
     3901 tgccttacct cggagggcag ctgggagagc ttccaacccg tcgtggggga aaaggcttcc
     3961 accaaggcat cgaaaggata ctgcagatac tggctgagga gatgaagctt cagggaggcc
     4021 acggcagtat cggtctgaac cagggggacg aggttgaact gggccaaccg gttcaccatc
     4081 gtccggtagt tggtggtctc tccgttgtgg gtcagggctg tatgtaattc ggcaaaggga
     4141 tgagcattca tttgatcaac caccgacccg gtcgccagcc gccggtggat tacgttgacc
     4201 ggagcctcgg gcagctccca gggaatcagc cgcccgtcgg tcttccagca acccgagttt
     4261 tttccgcagg acatgacggc ggctcttttt tctttgacct cggcgtagtt ctcctgggtg
     4321 attcgccggg aaaggcgata gatatactcg tcttccgcct cctgcaagaa atgccggttc
     4381 tggccataat ttccaattgt gacctctcgg tagcgcatct ctttgggggg ccatactgga
     4441 tcattcacta aagcctccac gatgaagcgg tggatctctt ccgggcgcac ccggacaaag
     4501 aatcggaaca tatctcccgg gtcttttcct ccgaaagagc ggaaatcctg ttcctccccc
     4561 tccaggtctc gccggtagga aatgcggtat ttctccatat ttccatcgaa cgcttcgatc
     4621 tccaggactt ttaaatattt ctccaaaatc tcccccatga ggcggcgcct ctcggctaat
     4681 attttttccc gggcccttcg ctggagctcc cgggggagga gtccgggata ttgagcctgg
     4741 atctcctgct ccgcctgagg ctgcagtgtt cctttgatca gcaagttgag ggcatagtga
     4801 tccagatgat ccggataaca tcctcccttc cagatgccca ccccgtccat cccccggttg
     4861 gccatgcggt cgagcatcag gtccatcaca tggctgggga tgaagtggtt ggaaaccacg
     4921 gcagcgaccc cgcagtcgga gggagcggcc agcggccccg ggaggatttc ccgggctgcc
     4981 ggtggccctt ctgaaagcat aggctggccg gcttcctgga tctgcgctct ccggcggagg
     5041 cgttccccca ctcgcccttc caataggggc caggtgacac aatcgtacct tccccggagc
     5101 tcccggatgt ccttcattcc caatgccccc agggctcgga tcacctgggc ctggaggatg
     5161 gcccggaagt tgatgatttg ccgggtgttc atttctatgt cccgcagtcg ctgcatgcgg
     5221 ggttcgaggg tggagatgcc gctgcggcat ccggactcgc agttgcggtc ccgcacgcac
     5281 ccgatggcca ccaaatcggc cgtgcccagg atgaccccgt ccaggcccat ggccacccct
     5341 ttgatgatgt ctgcccaggt acggaacccg ccgcttccgg tgaagaccac gaaatcccgc
     5401 agtccatcgt ccatgagctt ttcatggcag atgggaatgg catactccat gtgcattccc
     5461 atctggccct tgatgatgtc cggggccgcg cccgttcccc cgcggggtcc atccagccag
     5521 acttcgatca ccatccggtt gtctttggca atgcgggcca acccggacac gatgaattca
     5581 atatccggag agggagagac cttgatgcag atggttgtcc gggcgttgat catccgcacc
     5641 gcatcgataa aggctttcac gtcctcgatg gaatagcagt tgtgaaaagg aaagggggag
     5701 atgaggctgg tgccctcggc cacgccccgc aggcgggaga ccaggccgaa aaccttccgg
     5761 cccaaaagat ggccacccac tccctgctta gctccttggc tgtaggcgat caccactcgg
     5821 ggagcctttc ggatcgtctc ctgggtcacg ccgaatagtc cggtcttcag ttccgtggaa
     5881 atataggttt catcctccgg ggctacgatg ggcacaaatt ggacctccag agaggatgga
     5941 tatttggcga gggccttccg tattggggga taatctggca gggtggccaa ctcccggtcc
     6001 gcctgctcca ggagctcact caaaggaatg agtctcctct cctccaggac ccggctcagc
     6061 agggcggtct gcgaatcggt caggtccagg gggttccatt tgctgtccat taggtagagg
     6121 gtgggatatc cgccttcgcc ggtgtccagc tgggtctcca gggcccgcgt ggccagcacc
     6181 cgcgcccgcc aggtccccgg cccgatggaa cccacagaca tccccgctcc cagaaggggg
     6241 gtccgtaagg tcagggaatc ggcgaggtgc agggaggtgg aaatgtcctc caggctatct
     6301 tcatcgacct ggtccgggtc ggtggaaaag cggatccgca tgtcatcaaa cccatccccc
     6361 aaatttcccc gttcctccac gcagcctaag agcagggttt gcgaatcccc gctggccatt
     6421 cggccgacgg tctccagaag ggaggccggc cagacataat cccggtcaaa aatcttctgg
     6481 caggaatcct tgaggcacaa gctgtagaac cggtgcggca ggtctgccag ctggacccta
     6541 tccttgagct ggcggtatcg ccagccccgc cccgggccct cgcgctccat ccgtgcccgg
     6601 ttggtccggt gatttctctc ccggcgggag gggctgtgga agatgtcgta gaccctttct
     6661 tccaagtcgt caaagatgag aagacggttt tcctccccga cggttttttt aatgtccttg
     6721 aacccggagg cccctaagac ctctatgatc tgcacattcc aaacccggat gaggttgacc
     6781 agacgtttga ccaaaaggga agcatcttcg gggatggaca gtggctcctt gcggcgaaat
     6841 ttctgccaag ccatcgggtc ggcgacgaac agggccggcc agtcaatctt gatcccgttg
     6901 gcgccccgct gaatggtctt ctgggcgtcc gctgccgaac ggattccgcc cgaagccagg
     6961 atggagaagc gatgccgttc ctttttctcc accaggaaac gatgaaccgc gggaaccgcg
     7021 tcggtggtcg gataataccc tttgtctgga tggaattcgg aggtcaggca caagaggtcg
     7081 aaaatggagg ggaagaggga atccagcgtt tgccggaggc gagtttgggg atggagctcc
     7141 gcatccacat ccccgggggt aacccagact actgcgctaa agatcgtccc ttccgggaaa
     7201 agggtccgga gttcatgaga tcgctggatc acctcggcgt ccacctcgac ttccacccaa
     7261 tcgaagaagt gatctttcag cttggccgga agggttttcg cgctcaagag aaccttcgcc
     7321 tcttgagcgc tcaggcgcac gaggatatga cccgtcttcc cccggacccg gtttgccatc
     7381 cgtttcacct cttcaagccg cagcgtcaga cgggttccta attgtcctgc tgcctccagg
     7441 agggcggcga gcattccatg gcccccatcg acgggcatca cctccaggag gaggggtgtc
     7501 ttcaacttca ccaggcgagg actaggggtc tcgatctcat cgccccgaaa ggtcagatat
     7561 ttgggccgct tgcccagctg gacggcgata gcgtcctcgg agctttcgta gagaaggttt
     7621 tgggcggggc ccacgatgtg gaaatggccg aaacggatcc cgtcgttgcc caacccgcgg
     7681 tgcgggtctc cctggccggt cccggatacg gggatcttgc cggtggtggc ttccaggtcg
     7741 atgcggctga tgacctctcc gctccagtag gggtcctcca tttggagata ccgggggtgg
     7801 agttccacgg tggtggcagc caggccctcg gccacctggg ggcaccgttc caggcacgcc
     7861 ccgcagccgc ggcacagggt ctcatcggca accaccactt tccgggggtc cccctcgtcc
     7921 cgcttgcgca cgctgtacat gcaggcatcg acacaggttc cacagtggag acaatgttcc
     7981 tcatcgatgg ccacccggta gcgcgggaaa gtctggcgcg gctcctggat cttctggtcg
     8041 aagccggtac gaaccacatc caagaatctc tgaaagtccg gactctcttc cacctggcgg
     8101 ataaattcgg cgacggcctg gagggggaag ggaatggatt cggctaacac ctgctccaga
     8161 cgatcgaaga gcgggcggtg cttcaggttt acctggcatt cttcatcgca ccggccgcag
     8221 tgcaggcaga agtagagggg caggaccttg gccaattcga ttttcgacgc cctcagggcg
     8281 cgcttgacgt tgagcagctt tccccgggcc gtgaaggtct cgtcccccgt cgcccggtaa
     8341 acagggcagc gggccacgca cctgccgcac tgggcacagg agaggatatc gttggcgatc
     8401 tcttccattc cccgggaaac gaccgggagg cgccgtcgca agactttttc ggggaggaag
     8461 gtgaagagcc tgaaaagaag ccactgtatg gtggcaagtt cgaagttgaa gactccggac
     8521 tggaagagaa ccttctgtag tgagctgaac cgcccggaga cgccaaagaa ctttcccggg
     8581 ttaagcaggc ctgtggggtc cacttttttc ttgaactgca ccagccgctt cacctcccgg
     8641 ggcggaaaca gatcctggag gaagggcgta ttccagattc ccagcccgta gggtttgccg
     8701 ccgtagccct ggatggccag ccggaccatc atgggaacga ggaggagatc gatgtaaaag
     8761 atggccttgc ggtagtccga gggtatcatg accagcagaa ggactctctc cgggtcgatc
     8821 agatgggaga tgggatagaa ggttactccc aggcgctcgc cccagtcgcg gaccctctct
     8881 tgataggcgg ccacccgatc gagcggcagg accacctcgg cagccaggag agaggggccc
     8941 aagggtttga tggacaaggg gagggaacgc tcttcccaga gaaactgagc gaccttctct
     9001 tccgctggct tgattcccct ttgggtcaag taacgcctga accggtctgc aggctccgct
     9061 tgggcgaagc ccaccaggac caggttctgg gggtgcgaag aacccccgtc cccggaggga
     9121 atggggagaa ccctgactaa ttcagaatgg aatatgatta catcgtctgg ggcgatgttc
     9181 ggctgtttgg ccaggtcctt ggccaaggaa aaggcatcct tcgaatcaaa aaaagggatc
     9241 gcaaagggaa agtaggcagg tggccgttcg cccacccgga gggtggcctc gacaataacg
     9301 cccatctgtc cttcggtgga gacgaaatag ggaagatccg gatcgtcagc cttcagctcc
     9361 cggacctctc cggcaggggt gatcactcgg atggaaagga gctgtttggc cacagggccg
     9421 tggcggaaag tccccaggcc gatgccgcca gtgacgatcc acccggtgag ggtgccgcct
     9481 tttccagagg gtatggagag aagggtcaac ccgtggcggg ccaattcccg gtcgatttcc
     9541 acgaaccgcg cgcccgggcc ggcattcaat tgtcgtgcct ggacatccac ctggacctgg
     9601 ttcatcaggc cgaggtccat cagaattccc ccggaggtag gaaccgcacc cccgaatccc
     9661 ccggttcccg cccccctgag ggtaaggggg atctgatgcg cccgggcgaa ggctaatatt
     9721 tcccggatgt gcttttcggt ggtgggctga aaaaggatgt ccggcttcgt cttaagcatg
     9781 aggtcgcgga tcatcggcgg aaggtcggca ttgagatcct ggcggtagta ttctaacttt
     9841 tccgggtcgt cgacgactcg aagccccggg tttttttgca gtaattcccc gatctgttcc
     9901 ttccaatgag ggttgaagac caccttaggg ttcatctctc cccctatcac taacattcaa
     9961 aatctttaaa aatttttcgg ttgcgcctcg gccacgctgg gatcctcatg cccaaccggc
    10021 gcagaaagaa aattactttt atttattata taatatcatc ggccgggcag catagatcct
    10081 gaaccccaac ccccaggcct ttgaattccg actgcttccc aatgttatct gctcctgcca
    10141 ccgtttaccg tccccggaac cctcaatcct ccccgattat taccggtgtg tg
//
LOCUS       DASPRR010000168        11090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_528735, whole genome shotgun sequence.
ACCESSION   DASPRR010000168 DASPRR010000000
VERSION     DASPRR010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528735"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VEL68_06470"
     CDS             complement(<1..201)
                     /locus_tag="VEL68_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009728788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXZ35643.1"
                     /translation="MRSFVGIEPFVKAKTSLIGLGNILMGDDGVGVHAVEALKRKYDF
                     PEEVRLLDGGTLGLDLLHLIEGM"
     gene            713..1660
                     /locus_tag="VEL68_06475"
     CDS             713..1660
                     /locus_tag="VEL68_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXZ35644.1"
                     /translation="MKTRITELLGIKYPIIQGAMTWVSFPPLVAAVSNAGGLGILGAA
                     FMNPEQLRDNIRQIKDMTDKPFGVNFMPDNPIVDQLLDVILEERVPVASYGRGNPKRI
                     IERVKPYEIIALPTLGSLKHALRAEQDGADAVIVQGTEGGGHTGHIATMVLTPLVASR
                     VKIPVVAAGGIGNGRGLAAALALGAEGISMGSRFIVTKEAPVPEHVKRYLIDKSEEDT
                     VVTDNLTGVRCRVIKNDFAKRIMEMAKSKVDPWEMMQYGVGRIRKAYVEGDLEWGSMA
                     FSQACGLIDDIPSCQELLDCIVSEAEEIMKSMGKRVNHS"
     gene            1794..1931
                     /locus_tag="VEL68_06480"
     CDS             1794..1931
                     /locus_tag="VEL68_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35645.1"
                     /translation="MAEVELPNPHELEEVKEKGFTRRVAMITAVFAVLLAIASLGGRI
                     I"
     gene            1943..2380
                     /locus_tag="VEL68_06485"
     CDS             1943..2380
                     /locus_tag="VEL68_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HXZ35646.1"
                     /translation="MAQQQASNQWSYYQAKVIREHLYRSQMMLLETMLLDRGASMKAE
                     AKGQVEGMIKTMKEEEKRYNQEKKEIEQEAKKLEEKRDEHRAKDPFFEFGEVLLQIAI
                     VMSTVAILTHSAKIFSFAVGAAVLGALFALNGFFMILHLPLFH"
     gene            2523..3092
                     /locus_tag="VEL68_06490"
     CDS             2523..3092
                     /locus_tag="VEL68_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXZ35647.1"
                     /translation="MSTDKKEETFLLRLRPGLERELPMVTLAGTGKRIASFVMLGDVE
                     LNEKCAQMLVERFKAEGLLDKFDLLVALEAKGIALVHEVARQLGYPFFFVVRKSVKKY
                     MIKPLMVPVTSITSAGDQTLVLDGRDADRIKGHRVCLIEDVIATGGSVLAACDLIEYA
                     GAEVTVIATVLLKGDFHDPRLIYLQKPSL"
     gene            complement(3142..3846)
                     /locus_tag="VEL68_06495"
     CDS             complement(3142..3846)
                     /locus_tag="VEL68_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35648.1"
                     /translation="MLSVKKIGASYGYVQALSEVSLEVPERGTVTLIGSNGAGKSTLL
                     KVISGLLRPKRGSIEFLGERIEGLSPDKIVARGIAHCPEGRRLFPELTVLENLEMGAY
                     LSKSKSFVQERIEQLFDSFPILKERRGQLAGTLSGGEQQQLAIARALALNPKMVLFDE
                     PSLGLAPILVDQVEKILLQLKEERITILLVEQNASMALDVADYAYVMETGGIILQGAS
                     ADLRQDEKIVKAYLGG"
     gene            complement(3833..4615)
                     /locus_tag="VEL68_06500"
     CDS             complement(3833..4615)
                     /locus_tag="VEL68_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35649.1"
                     /translation="MAGELLSVQRLTVRFGGLTAVNDLDFSIFPGEIVSLIGPNGAGK
                     TTTFNAITGFLRASAGSVRFEGAELIHLKPHQIAEKGITRTFQITSLFPNLSALENIR
                     TGHHMQERETFWDAVLNTRRKRRVEDETLARTAEILKFIGLEGKKGAMAGSLPYGDQR
                     ILEIGVALAANPRLLLLDEPSAGLNDTETQGMKELIQKMRARSITIFLVEHDMKLVMG
                     LSDRIVVLNFGKKIATGTPEQVKTNPEVITAYLGDRKRDAKR"
     gene            complement(4605..5561)
                     /locus_tag="VEL68_06505"
     CDS             complement(4605..5561)
                     /locus_tag="VEL68_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35650.1"
                     /translation="MRRTSATAALCLAGAMVYPIFFADPYFLHMAILMGIYALLAVSL
                     NLLLGYTGQLSLGHAAFFGVGAYLSSLFHLHLGVPLWGGMVLAVCGAGVLGFFVAKLA
                     FKLRGAYFVIMTIGIAEVVKLVALNWVDLTKGPMGLTDIAAPQLSFGFITIDFSQKVP
                     YYYLILLCVVLAAVLQHRLVNSTLGRSFIAIRENEDLAESVGIDCYKYLVIVMVVSSS
                     ISGIAGSLYAHYVSFIDPGVFAFFISVNAVMMAIGGGQGTQAGPILGAILFTLIPEWL
                     RVFGDARMAVYALIVIFIVIFMPKGMLYSLAQIIQGKKNDGR"
     gene            complement(5558..6427)
                     /locus_tag="VEL68_06510"
     CDS             complement(5558..6427)
                     /locus_tag="VEL68_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35651.1"
                     /translation="MVFIQYVINGLIIGGAYALIGIGLTLILGIMKVVNFAHGEMYML
                     GAYFLFTFFSKMGIYFLLSMALAILAVMAIGWVFEKGILKPLRRRSIDTVMLSMIGLS
                     LFMQNIAMLIWTPVPESIQSPLPMTTYTLGGLYFLPQRILVICAALLLVLLTHLLLKR
                     TKVGKAMRATFQDLEAASLMGIKIDRIYSFTFVLGAGLAAASGVLLGPIFTVHPYIGN
                     LANLKAFVIVIIGGLGSFWGAVTAGLLLGVFESLGAAYVSSGYQDAVGFILLILILIK
                     RPTGLFGKVGQMR"
     gene            complement(6581..7744)
                     /locus_tag="VEL68_06515"
     CDS             complement(6581..7744)
                     /locus_tag="VEL68_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35652.1"
                     /translation="MERRVIRSVFILSFIFLFSGAALAAEPVKIGVVYPLTGPAAAAG
                     SYQKAGVEIGRDKINAEGGIAGRPVTLFIEDGANDPAQSVSAAEKLVTRDKVDAMIAA
                     WGSSPTLAVSASVTKKYGIPHVVDTASSVKVTMLDGKRPNPWLFRISPTSRMEAEGLE
                     KVLVPKMGFSKVAFMAVNNDWGRGAAEEFAKIVTKTGGSVVATEIINADATDFLTQLT
                     KIKSAGANAIIVTTDQAQVSLILKQYKQLGMDQKILTTGGGIVPEGLILLSGPEAAEG
                     VNIITFFAPWFPEMAKNPAEAKWFSEEYLKRGFAAKGFGECFRGYDGMKAVKAAMDLA
                     KTTDKEKVREAFSKVDTWGLSGRIKFDEFGQSSPNILVIQVKNGKPFIPEFMK"
     gene            complement(7881..9776)
                     /locus_tag="VEL68_06520"
     CDS             complement(7881..9776)
                     /locus_tag="VEL68_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ35653.1"
                     /translation="MDDFGYMGLICRVNLTTAKVSAEPLVERDLERFLGCSGYAAKML
                     WDELEAKVDPLSERSKIILSTGPLTGSLCPSGGSYELCFKSPLTGAWCQARSGGSFGP
                     RLKKGGFDHLIVEGRSDRPVYLWIRNGRVEIREAAHLWGKGVDQTTDAVHKEVGDPDA
                     SVAAIGAAGENQVRFAALINDRGRAAGRGGGGAVLGSKNLKAIAVNGTMDLRVARPEE
                     FIRAIQKAEEALDKYPFENINQYGTSLLVNILNANGCLPTRYFRFSTFEGAEDISGEL
                     LAEKYLIKRRACYGCSMGCGRYAGVENGPWATPPQEGPEYETINMLGSLCGNNDLASI
                     IRANHLCNNLGMDTISAGSVIAFAMECFERGILTKKDLGGLALHWGDSEGILRLLKQI
                     AYREGIGGLLADGVRSAAARLGAEEFALHTKGLELPAHEPRGESAILGLQYAVSHRGA
                     CHMHPNWASTWDAGKFESGLREFGLPWPPADKFAETGHQKGLAYRFVVLQGEIAEILG
                     CCIFHSWGAADQCLTPQLYGEMLRSLTGRPVTNKGLMLAAERSWNLKRCFNVREGLTR
                     KDDRLPGRMFEPLPDGPNKGRHMKDLEGMLDEYYDAMGWDREAGIPTKETLRKLDLED
                     IGNALGK"
     gene            complement(9790..10281)
                     /locus_tag="VEL68_06525"
     CDS             complement(9790..10281)
                     /locus_tag="VEL68_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013266611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ35654.1"
                     /translation="MLKTLIALPERCTGCHRCEMWCSLKHKGVINLERATIHVLRREP
                     SIDDPRVCLHCGICISSCSKNLIKRNQKTGAVEIDTQECTSCGHCVLSCPYGMITLDP
                     VDRHAVKCDQCGGQPECVKHCREEAILYVPADKAALHRREAIARKLGEERQKPNLRGI
                     KTG"
     gene            10571..>11090
                     /locus_tag="VEL68_06530"
     CDS             10571..>11090
                     /locus_tag="VEL68_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXZ35655.1"
                     /translation="MEKEQFSCVGHSVPKVDALDKVLGRAVYSEDISFPDMLHGRVLR
                     AGIPHAIIEEIDTEEAGAMEGVACVLTARDIPGKNRFGIAFQDQVALAEDRVRYIGDA
                     VALVAAETEEIAKDAVKAIRVKYRPLPVITSPREGLAEGAVKIHEKGNLLLHTKLRKG
                     NVAEGFARADVVV"
BASE COUNT         2362 a         3201 c         3156 g         2371 t
ORIGIN      
        1 cattccttcg atcaggtgga ggaggtcgag ccccagggtt cctccgtcca ggaggcgcac
       61 ttcttcgggg aaatcatatt ttcttttgag agcctccacg gcgtggacgc ccaccccatc
      121 gtcccccatc agtatattgc cgaggccgat cagtgaggtc ttagctttca cgaaaggttc
      181 aatacccacg aaagaacgca tcctttgaca tccgttgcga attgtcctta gagcttttca
      241 ttattattac acctccggcc gggacggagg tcaatgactg gcaacagtag acaataatat
      301 atttcagggg gaaaagaggg aaagtcaaga caattttggg cggaaaacaa attcttgaca
      361 aagagaaaag gggggtctta atatatagta tagcagagga ggatgagatc tgtggatcag
      421 gtattggttc agctttgggt tcttggctta ctcctgttgt gcctcgccct cgggcccggc
      481 ccactgcctg aacgttcggc ttgatccgca agcagaaaaa atgaaacgat tcaggccgtg
      541 gtcgccaccc acggccgaat gatagagagg ccgtggggag aaccctcacg gccttttcgt
      601 tatttccggg tcagaagtat tcgccactgg acccggcaga ctggaaatca gatatcgggg
      661 tcaaatccag aacccggcat cgagtatcca gtatctgtca caaggaaatg gaatgaagac
      721 gcgaatcaca gagctgctgg gaatcaagta tccgatcatc cagggggcca tgacctgggt
      781 gtcttttcct cctttggttg cagcggtctc caatgccgga ggcctgggaa tcctgggcgc
      841 tgcgttcatg aatccagagc agctccggga caatattcgc caaatcaaag acatgacgga
      901 caaacctttc ggggtcaatt tcatgcccga caacccgatc gtggatcagc tgctggatgt
      961 gattctggag gaaagagtcc ccgtggccag ttatggccgc gggaatccca agcgaatcat
     1021 cgagcgggtg aaaccttatg agatcatcgc tcttcccacc ctgggctccc tgaagcatgc
     1081 tttgcgagcc gagcaggacg gggccgatgc ggtcatcgtc cagggaaccg aggggggtgg
     1141 acacacgggg cacatcgcca ccatggtcct gactccattg gttgccagcc gggtgaaaat
     1201 tccggtggtg gccgcggggg gaatcggcaa cggccgagga ttggcggccg ccctggctct
     1261 cggcgccgag gggatctcca tgggaagccg gtttatcgta accaaggaag cgccggtccc
     1321 cgagcatgtg aagagatatc tgatcgacaa atcggaggaa gatacggtcg tgaccgacaa
     1381 cctcaccggg gttcgctgcc gggtcatcaa gaacgacttt gccaagagaa tcatggaaat
     1441 ggccaagagc aaagtcgatc cctgggagat gatgcagtac ggggtgggcc ggatccgcaa
     1501 ggcctacgtg gaaggggacc tggagtgggg gtccatggct ttcagtcagg cctgcggctt
     1561 gatcgatgac atcccctcct gccaggagct gctcgattgc attgtgtccg aagccgaaga
     1621 gatcatgaag tccatgggga agagagtcaa tcattcctga gcctttcctc gaatttcttc
     1681 cccgtcctcg cgaaaagctt attttctttc cgtccttttt ttgctaacat aatccgccaa
     1741 tctgcttttt tcctgttatc ccatttcctg agagagagga ggaaagaaaa tatatggccg
     1801 aagtcgaact tcccaacccc cacgagcttg aagaggttaa ggaaaaaggc ttcacccgca
     1861 gggtggccat gatcacagcg gtctttgccg tccttctggc cattgcttcc ctgggtggac
     1921 gtataatatg aaggaaatgc tcatggccca gcagcaggcc tccaaccagt ggtcctacta
     1981 ccaggccaag gtgatccgcg agcatctcta ccggagccag atgatgctcc ttgaaaccat
     2041 gctcctggac cgcggggcct ccatgaaagc agaggccaaa gggcaggtgg agggaatgat
     2101 caaaaccatg aaggaggaag agaagcgcta caaccaggaa aagaaggaaa tcgagcagga
     2161 ggccaagaag ctggaggaaa aaagggacga gcaccgggcc aaggacccct ttttcgaatt
     2221 tggcgaggtt ctgctccaga tcgccatcgt catgtccacc gttgccattt tgactcattc
     2281 agcgaagatt ttttctttcg ccgtcggggc cgccgtgctg ggcgcgcttt tcgccctgaa
     2341 cgggttcttc atgattcttc accttcctct attccactaa aattcccggt cctccggtgt
     2401 ttaacttgac agcagcggca tgttacaaag cgaccataaa aaaatagttc taggatgcgc
     2461 cttcctatga tcggtaagga agcgccaaga agcggctata ttctaaattc atcccagaag
     2521 gtatgagcac ggacaaaaaa gaagaaacct tcctccttcg cctccggccc ggtttggagc
     2581 gtgagcttcc gatggtcact ctggcgggga ccggcaaacg catcgcctcc ttcgtcatgc
     2641 ttggtgatgt ggagctcaat gagaaatgcg ctcagatgct ggtggaaagg tttaaagccg
     2701 agggactgct ggacaaattt gacctgctgg tggccctgga ggcgaagggc atcgccttgg
     2761 tccatgaagt ggcccgacaa ctgggctatc cctttttctt cgtcgtccgc aagtcagtaa
     2821 aaaaatacat gatcaagccc ttgatggtcc cggtcacgag cataacgagt gcgggagatc
     2881 agaccctggt tttagacggg cgggacgcag accggatcaa ggggcatcgc gtttgtctga
     2941 ttgaagacgt tattgccacg gggggatcgg tcttagccgc ctgtgatttg atcgagtatg
     3001 ccggcgcaga agtgacggtc atcgccacgg ttctcctgaa aggggatttc cacgatcctc
     3061 ggttaatcta cttgcagaaa ccctccctgt gagtctcctc catacctcga aaatcatgac
     3121 ctccagatag gggagcaggc tttagcctcc cagatatgct tttacgattt tttcgtcctg
     3181 gcgcaaatcg gcagaggccc cctgaaggat gattccgcct gtctccatga cataggcata
     3241 atcggcgaca tccagcgcca tgctggcatt ctgctccacg agaaggatgg tgatcctttc
     3301 ttccttcagt tgcaggagga ttttttcaac ctggtccacg aggatggggg cgagtcccag
     3361 ggagggttca tcgaagagga ccattttagg attcagagct agcgcgcggg cgatggccag
     3421 ctgctgctgt tcgcccccgc tgagggtccc ggccagctgg cccctccgct ccttcaggat
     3481 ggggaacgaa tcgaaaagct gttcgatcct ctcctggaca aaactctttg atttggaaag
     3541 ataagccccc atctccagat tctcgaggac cgtcaattcc ggaaagagac ggcggccctc
     3601 ggggcaatgg gcgatgcccc tggcaacgat cttatccggg gatagccctt caatccgctc
     3661 ccccaagaac tctatcgatc cccttttggg ccgcagcagt ccagagatca ccttgagtag
     3721 agtcgatttt ccggctccgt tcgatccgat caaggtgacc gtccctctct cggggacttc
     3781 cagcgagacc tccgacaggg cttgaacata cccgtagctg gccccgattt ttttaacgct
     3841 tagcatctct tttccgatcc ccgaggtagg cggtgatcac ctcgggattg gtcttgacct
     3901 gctccggcgt tcccgtagcg atctttttgc cgaaattcag gaccacgatg cggtccgaaa
     3961 gccccatgac cagcttcatg tcatgctcga cgagaaagat ggtgatggac ctggcccgca
     4021 ttttttggat cagctctttc atgccctggg tttccgtatc gttcaagcct gccgaaggtt
     4081 cgtccaggag gagcagccta ggattcgccg cgagggcgac tccgatctcg agaatccgtt
     4141 gatccccata gggcaggctt ccggccatcg cccccttctt gccctccagg ccgatgaatt
     4201 tgagaatttc agcggtccgg gccagggttt catcctccac ccggcgtttt cgccgggtgt
     4261 tcagaacggc atcccagaac gtctccctct cctgcatgtg gtggccggtg cggatatttt
     4321 ccaaggccga caggttgggg aaaagggagg tgatttgaaa ggtccgggtg attccttttt
     4381 ccgcgatttg gtgaggcttc aggtggatca gttccgctcc ctcgaatcgg accgatccgg
     4441 cactggcacg caaaaagccg gtaatggcgt taaaggtagt ggtcttgccc gccccgttcg
     4501 ggccgatcag gctgacaatc tctccgggaa aaatggagaa gtccaggtcg ttgaccgcgg
     4561 ttaagccgcc aaatcttacg gtcagtcgtt gtacactcag cagttcaccg gccatcgttc
     4621 tttttccctt ggatgatttg ggcgagggaa tacagcatgc ctttgggcat gaagatcacg
     4681 atgaagatca cgatcaacgc atacaccgcc atccgggcgt ccccgaagac cctcagccat
     4741 tccgggatca gggtgaagag gatcgccccc aggatcggtc cggcctgggt gccctgcccc
     4801 ccgccgatgg ccatcatcac cgcgttgacg ctgatgaaga aagcgaagac accgggatcg
     4861 atgaagctca cgtaatgggc gtacagggag ccggcgatcc cggagatgga ggaactgacg
     4921 accatcacga tgaccaggta tttatagcag tcgatgccca cggattcggc caggtcttcg
     4981 ttttcccgga tggcgatgaa ggatctcccc agggtggaat tcaccaaccg gtgctggagg
     5041 acggccgcca ggacgacgca aaggaggatc agataatagt agggcacctt ctggctgaaa
     5101 tcgatggtga taaacccgaa actgagctgc ggggcggcga tatcggtgag ccccatgggc
     5161 cctttggtca ggtcaaccca gttgagggcc accagtttca cgacttcagc gatcccgata
     5221 gtcatgatta caaaataggc cccccgaagc ttgaaggcca gcttggcgac gaaaaagccc
     5281 aggaccccgg ccccgcaaac ggccaggacc attcctcccc acaggggaac ccccagatgc
     5341 aggtgaaaca gggaagagag gtaggcgccc accccgaaga aggcggcatg cccgagggag
     5401 agctgacccg tgtaccccag gagcaggttg aggctgacgg cgagcagagc gtagatcccc
     5461 atgaggatgg ccatgtggag aaaatagggg tcggcaaaaa agatcgggta gaccatggcc
     5521 ccggcgaggc atagagcagc ggtggcgctc gttcttctca tcgcatctgt cccacttttc
     5581 caaacaggcc ggtcggcctc ttgattaaaa taagaatcag gaggataaag cccacggcat
     5641 cctggtatcc ggatgagacg taggcggcac ccaggctttc gaacaccccc aggagaaggc
     5701 ccgcggtcac ggccccccaa aagctaccga gacctccgat gatcacgatg acaaacgcct
     5761 tcaggttcgc caggttgccg atgtaagggt ggacggtgaa gatcggcccc agcagtacgc
     5821 ccgaggcggc cgccaatccc gcccccagga cgaaggtaaa ggaataaatc cggtcgatct
     5881 tgatccccat cagcgaggcg gcttccaggt cctggaaagt ggcgcgcatg gccttgccca
     5941 ccttggtcct tttcaacagc aggtgagtca ggaggaccag gaggagggcg gcgcagatga
     6001 ccaggatccg ttgggggaga aaatagagcc cgcccagggt gtaggtggtc atggggaggg
     6061 ggctctggat ggattccggc actggcgtcc agatcagcat ggcgatattc tgcatgaaga
     6121 gggatagccc gatcatggag agcattactg tatcgatcga cctccggcgc aagggtttca
     6181 agatcccctt ttcgaagacc cacccgatgg ccatcactgc caggatggcc aaggccatgc
     6241 tcaaaagaaa atagatcccc atcttggaga agaaagtgaa gaggaagtag gcccccagca
     6301 tgtacatttc gccatgggcg aaattgacca ccttcatgat acccaggatg agcgtgagcc
     6361 cgatcccgat cagggcgtag gctccgccaa tgatcaggcc gttgatcaca tactgaatga
     6421 agaccatatt ccgctactca gaatgaaggc ctgaaaggca gggaatggac accggacagg
     6481 gcataaaaat acccgccaat tcccccctct ccctcgaaaa aggatgaggg gggaagggta
     6541 aggggaaaaa tcccgggtgg ccgcagaaat tcaccgcccc ctatttcatg aactcgggga
     6601 tgaaaggttt tccgttttta acctgtatga ccaggatgtt gggggagctc tggccgaact
     6661 catcgaattt gatgcggccc gagaggcccc aggtgtccac tttggaaaaa gcctcgcgca
     6721 ccttctcctt gtccgtcgtc ttggccagat ccatggccgc cttgaccgct ttcatgccgt
     6781 catacccgcg gaagcattcc ccgaagccct tggccgcaaa cccccgcttt aggtattcct
     6841 cggagaacca cttcgcctcc gccgggttct tggccatttc cgggaaccag ggggcgaaga
     6901 aggtgatgat gtttaccccc tccgcggctt ccgggccgga aaggaggatc aatccctcgg
     6961 gaacgatccc acctccggtg gtcaatattt tctggtccat ccccagctgt ttgtactgtt
     7021 tcaagattag cgagacctgg gcctggtccg tggtcacgat gatcgcgttg gcccccgcgc
     7081 tcttgatttt cgtcagctgg gtcaggaaat ccgtggcgtc agcgtttata atctccgttg
     7141 cgaccacgga gccgccggtc ttcgttacga tcttggcgaa ttcctcggcc gcgccccggc
     7201 cccagtcgtt gtttacggcc atgaaagcca ctttgctgaa acccattttg ggaaccagca
     7261 ccttctccaa tccctcggcc tccatccggc tggtcgggct gatccggaag agccatgggt
     7321 tcggcctctt tccgtccagc atcgtcacct taaccgagct ggccgtatcg accacatggg
     7381 gaatcccgta tttcttggtg acgctggcgc tgaccgctag ggtgggggag ctgccccagg
     7441 cggcgatcat ggcatccacc ttatcccggg tgaccagctt ttccgcggcg ctcacggact
     7501 gggccgggtc attggcgccg tcctcgataa agagggtaac gggccggccg gcgattcccc
     7561 cttcggcgtt gatcttatcc cggccgattt caacccccgc cttctgatag ctaccggcag
     7621 cagcggccgg ccccgtgagg ggataaacga ccccgatctt taccggctcc gcggccaagg
     7681 cggccccgct aaaaaggaaa atgaacgaaa ggatgaatac cgacctgatt actcgtcttt
     7741 ccatttctaa cccctcctct tgaatttgga tttctgatga ggccatcatg catcccggcc
     7801 tcgattttac ccctatccgt tcctgatttc tgccgccggc ccggtccgat ccgggtcccg
     7861 tccccaaaaa ggaaccggtt tcactttccc aatgcgtttc cgatatcttc gaggtccaat
     7921 tttcggagag tttccttggt gggaattcct gcctctcggt cccagcccat tgcgtcgtaa
     7981 tactcgtcca gcatcccttc caggtctttc atatgccgtc ctttgttcgg gccgtcggga
     8041 aggggttcga acatgcgtcc gggcagacgg tcgtcctttc tcgtcagccc ttcccgcacg
     8101 ttgaagcacc ttttcaggtt ccagcttctc tccgcggcga gcatcagtcc tttattggtt
     8161 accggccgtc ccgtcaagga acgcagcatc tctccgtaga gctgcggggt gaggcattga
     8221 tcggcggcgc cccaggaatg aaagatgcag cagcccagga tctcggcaat ttctccctgc
     8281 aatacgacga aacgataggc gagccctttc tggtgtccgg tctcggcgaa cttatccgcc
     8341 ggcggccagg gcaggccgaa ttccctgagg cccgattcga atttaccagc atcccaggtg
     8401 ctggcccaat tggggtgcat gtggcaggca ccccggtggg atacggcata ctgcaaaccc
     8461 aggatcgccg attcccccct gggctcatgg gccgggagtt ccagtccctt ggtatgcagg
     8521 gcgaactcct cggctcccag gcgggcggcg gcggagcgaa cgccgtcggc caatagaccc
     8581 ccgattcctt cccgataggc gatttgcttc agcagcctca ggatgccttc actgtccccc
     8641 caatgaaggg ccaggccccc gaggtccttc ttggtgagga tccctctttc gaaacattcc
     8701 atggcaaagg cgatgaccga gccagccgat atggtgtcca tgcccaggtt attgcagaga
     8761 tggtttgccc ggatgatgga ggccaggtcg ttgttgccgc aaagggagcc gagcatattg
     8821 atggtctcgt actccggccc ttcctgcgga ggggtggccc agggcccatt ctccacgccg
     8881 gcataccgcc cgcatcccat ggagcagccg taacaggccc ttcttttgat caggtacttt
     8941 tcggccaata attcgccgga gatatcctcc gctccctcaa aggtgctgaa gcggaagtat
     9001 cgggtgggaa gacagccgtt ggcgttcagg atgttaacca gcagggaggt cccatattga
     9061 ttgatgttct cgaagggata tttgtccagg gcttcctcgg ccttttgaat ggcccggatg
     9121 aattcctccg gccgggccac ccggaggtcc atcgtcccgt tgacggcgat ggccttcagg
     9181 ttctttgacc ccaggaccgc cccccctccc ccgcgtcctg cggcccttcc ccggtcgttg
     9241 atcagcgcgg cgaaacggac ctgattttcc ccggcggcgc cgatggccgc caccgaggca
     9301 tccgggtcgc ccacctcctt atggacggca tcggtggttt gatcgacccc cttcccccat
     9361 agatgagcgg cctccctgat ctccacccgg ccattcctga tccagagata gacgggccgg
     9421 tcggatcggc cttcaacgat gaggtggtca aagccgcctt tcttcaacct cggcccgaaa
     9481 ctccccccgg aacgggcctg gcaccacgcc cccgtcaaag gggatttgaa gcatagctcg
     9541 tagctccccc cgctgggaca aaggctcccg gtaagcgggc ccgtggacag gatgattttg
     9601 cttctctcgg acagggggtc taccttcgct tccagttcat cccataacat tttagccgcg
     9661 tagccggagc agcccagaaa cctttcgagg tcgcgctcta ccagcggctc ggccgacacc
     9721 ttcgccgtag tcaggttgac cctgcagatc aggcccatat acccgaaatc atccatggtc
     9781 acccgcccct tatcccgtct tgatcccccg gaggttgggt ttttgacgtt cttccccgag
     9841 cttccgggcg atcgcctctc tgcgatgaag ggcagccttg tccgcgggga cgtacaatat
     9901 cgcctcctcc cggcaatgct tgacgcattc cggctgcccg ccgcattgat cacacttaac
     9961 cgcgtgccgg tcgaccgggt cgagggttat cattccgtac gggcaactta gaacacagtg
    10021 cccacaggag gtgcattctt gggtatcaat ctccacggct ccggtttttt ggttcctctt
    10081 gatcaggttc ttcgagcatg agctgatgca gattccacag tgcaggcaga ccctcggatc
    10141 gtcgatagac ggttctcttc gcaagacgtg gatcgtcgcc cgctcgaggt tgatgactcc
    10201 tttatgcttc agagaacacc acatttcgca gcggtgacaa ccggtacatc tctccggcag
    10261 ggcgatcagc gtcttcaaca tcgctttcct cttctttggt agaatcccgg cgtgctccaa
    10321 tttttatagc atattgatgg ataaagtaaa gcccttcggt gactgtcgtt cattcaggaa
    10381 accggacccc gaagatttac tcggaaggcc gctgtccagg gattccttca agccgggtgg
    10441 gaagcgggcc ttatttgctt gacatttaga ttatgggcca ataaactttt ttcatccagc
    10501 cccgggaagg ctttgaaaaa taaagcgttg ccctgcgacg ccctcgggga cccggatgga
    10561 gtgcgaggag atggaaaagg aacaattttc ttgcgtgggg cattccgttc cgaaggtcga
    10621 tgccttggac aaggtcctgg gccgggcggt ctattccgag gatatctcct tcccggatat
    10681 gctccacggc cgggttttgc gggcggggat tccccatgcc atcatcgaag agattgacac
    10741 ggaggaggcc ggggccatgg aaggagtggc ctgcgttctc accgcccggg acatccccgg
    10801 caaaaaccgt tttggcatcg ccttccaaga tcaagtggca ctcgccgagg accgtgtgcg
    10861 gtacatcggg gatgccgtgg ccctcgtggc cgccgagacg gaggagatcg ccaaggacgc
    10921 ggtcaaagcc atccgggtca aataccggcc ccttccggtg atcactagcc cgcgggaagg
    10981 actggccgaa ggggcggtca aaatccacga aaaaggaaat ctccttctcc acacaaagct
    11041 ccgcaaaggg aacgttgccg agggattcgc ccgcgcggac gtggtggtgg
//
LOCUS       DASPRR010000169        11007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2297495, whole genome shotgun sequence.
ACCESSION   DASPRR010000169 DASPRR010000000
VERSION     DASPRR010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297495"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2717)
                     /locus_tag="VEL68_06535"
     CDS             complement(<1..2717)
                     /locus_tag="VEL68_06535"
                     /inference="COORDINATES: protein motif:HMM:NF023400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HXZ35656.1"
                     /translation="MKKASLALLSFFLFFLTLHLDLHLNPGLTLWAQDTPRVENFTPQ
                     GTVKNIRQVRARFSEPMVPFGEPRDLAEPFAVSCPEKGTPRWADPKNWVYDFEKDLPA
                     GVRCEFTLKHGLKTLAGKEITGAETFAFSTGGPSVRESIPNQGNESIDEEQVFILRLD
                     AEPEPESILKNVTFSVEGLPERVGITLVEGKERKKILDSGRARWISRNLKESRHFLLL
                     IQSRQRFPNKAKVNLVWGKGVQTRTGVATETEQVLPFKTRDAFSVVFRCQRENPRGAC
                     LPITPMSLDFSAPVPAKWAQQIVVKGPGEKVWKPQLPEAETNYFNEITFKGPFPENAA
                     FRVELPAGLKDEAGRPLVNADKFPLAVRTDRYPPLAKFSSRFGILELNADPALPVTLR
                     NLEPQVKGRMVKADGEPGIFGSLIGKILSLPPDKKVEVQAWLRKVAAASREKSLLGDE
                     NAANSFQVPKPGGGQAFEVVGIPFKKPGLYIVELESSILGEALLDPPRPMYVSTAALV
                     TNLSVHFKWGRESSLVWVTTLDKGEPVKDAQVTVRDCQEKILSEGKTDAEGLFRIQGP
                     LPSGQDLPRCDHSIDSHDYSQAQALHLQGGFLVMAQTQDDMAFVHSSWNQGIEPWRFQ
                     LPYDDYRGPVMGHTVFDRTLFRAGQTVSMKHILRQRTMQGFSPVAEARRPDLVSIQHG
                     GSGEKYEFPLKWDAQGIAESAWVIPRDAKLGNYNVILMKKGSGEGRGEWRRVSGDFRV
                     EEFRVPLLRGMIKPPGEPLVKPKEVPLDLSVQYLAGGGAGMLPVRLRSEVRPRSIPPF
                     EGFDRFVFSNGPVKEGLVRRGADFEEEEEGEEEETVVARKGFKIPSQDLVLDRAGSAR
                     TALEKVPSFETPMEILTEMEFKDPNGEIQTVSTRMPLWPSHYLIGIKPDS"
     gene            2924..4435
                     /locus_tag="VEL68_06540"
     CDS             2924..4435
                     /locus_tag="VEL68_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HXZ35657.1"
                     /translation="MPYSKYPLISAEEAASNIPSGATVAFSGFSSAGAAKAVPRAIAK
                     QARSLHGRGEAYRIRVLTGGSTGKSIDEELAGAEAISWRAPYQSSPILRRQINRGEVE
                     YIDMHLSHVPQMVANGFFGKLDFAVIEATEITPDGRVFLTTSIGSSPTFLKHAERVII
                     EINPYHSMRLREIADIAIMPSPSHRSPIQIHDPLARIGHSYAAVDPRKIIGIVENQEP
                     DLMASFEPPSASSKKIAEHITRFLLEEIRTGRLPEKLLPFEAGIGDISNGVVSTLGKN
                     EEIPPFQMYTEVLLDSLVSLMEKGRVLGASATALTVDPDTLKGIYENMDFFVPRILLR
                     SQEFSNNPGIIRRLGVIAMNTALEVDLYGNTNSTHICGTDLMNGIGGSMDFSRNGYIS
                     FIMCPSISKGGKISNVVPMCSHVDNTEHSVSIIVTDQGLADMRGLGPAQRAKNIIEKC
                     AHPAYRPYLHRYLEKARKGHLRHNLQQCFELHNNFLEYGAMLPDLDLAGLERP"
     gene            4608..5729
                     /locus_tag="VEL68_06545"
     CDS             4608..5729
                     /locus_tag="VEL68_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ35658.1"
                     /translation="MSAKIKSVFVLGLGKVGLLVATLLHETAFRVMGADLREAMGLPF
                     PTVKLHLSNPEELKKRMEGFDAVVSCLPYNFNIGVASAAHELGMHYFDLTEDVRAANS
                     ILQMSLTARAVMAPQCGLAPGFIAIVGASLAETFDGIRSIKLRVGALPQNPTGLLGYA
                     FNWSPEGVVNEYLNDCEVIEDGEIKWVSPLEWLETIVIHGVQLEAFTTSGGLGTMCQT
                     YKGRVENLDYKTIRYPGHAKLMNFFFHELLMRKDRQKAGEILTCAKPPVTEDVVYIHA
                     SVEGWKGGSLSRDEFVRAYYPLEIAGQKRRAISWTTAASVCAVVEMVSNGSLPGKGFL
                     KQEEIPLAEFLKTNNGRLYAGEPLGKNLEGRNRQGGGKD"
     gene            5824..7227
                     /locus_tag="VEL68_06550"
     CDS             5824..7227
                     /locus_tag="VEL68_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HXZ35659.1"
                     /translation="MNSKAWKLGICVLVAVVILLLPVPAGLSPAAWQLFAIYLAAIFG
                     LVLRPFDEAVILLGVIAAAGLAFGNMGALLSGYASSSAWLVFSAFMIGAAFSATGLGR
                     RVAFFLIGKFGRTTLRLGYVAAVTDLAISPATPSNTARTGGIVYPIFQSIAIALDSKP
                     GPTARRIGAYLTLTLYYISFTTGYTFLTAMAPNVLILSFAKSILKVQVDWILWAKAAI
                     VPGLVTLFLIPWLVYKIYPPEIKKIDNETLGKKGLAELGPMSRREMTLAILFVLAVLG
                     WALGSVFKIDATAVAVGFVAACLLTGVMTWENVIAYKGAWSTFMWYGGIIGLADALSK
                     GKFFEWLAKLIAANVSFAGIHPLAILAALVLFSLAIRYLFASMAAYVATMIPVLFTIA
                     LVAKVPLLPLIFLIAFAAGYGGMLTHYGGALSPVLFGMGYIDQGTWWRKGAIMALISF
                     VTTFVFGFLWWNMIGLW"
     gene            complement(7375..7779)
                     /locus_tag="VEL68_06555"
     CDS             complement(7375..7779)
                     /locus_tag="VEL68_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35660.1"
                     /translation="MMKANQIWRIDDLRDKLAKTSLKMRKKGSQAGPDRLSPPACMVQ
                     VVAPAGYHQVQRIADAFGVDFDEQFSNEYEWDRFRQELSDLKVRVEQELQGRLELPEG
                     TFFCLGYDQEGNFGLILRSEDIALGNGGERMN"
     gene            8029..8379
                     /locus_tag="VEL68_06560"
     CDS             8029..8379
                     /locus_tag="VEL68_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ35661.1"
                     /translation="MERKEILVDPNKEEWEKIALPGIRIKYLYKNEQTGASMALCRFE
                     KGSGIPEPHLHASNQFMYVLKGRFTYPGIEVKEGMLYINPKDNVHGPSLALEDSLVLE
                     IYDGPHYYPEKKPY"
     gene            8521..9330
                     /gene="ygiD"
                     /locus_tag="VEL68_06565"
     CDS             8521..9330
                     /gene="ygiD"
                     /locus_tag="VEL68_06565"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HXZ35662.1"
                     /translation="MSALKSETEGSKKLMPALFVGHGHPMNAIQQNTFTAALSKAGEN
                     LPRPRAILVVSAHWLTRNNVIVSATERPRTIYDFGPFHPDLFKIVYAPPGAPEQARRT
                     ARMVNSSKVGVNTVRGLDHGAWTILKYLFPTADIPVFQLSIDFSQPPRFHYDLGRELL
                     PLRAEGVLIIGSGNIVHNLMQITWDHVDAPTEGWAKSFDRTVAELLRKGGHDELIEYE
                     RLDYAETAVPTNDHYLPLLYSIALQEKGEKIEFFYEGFQYANISMRCFRIG"
     gene            9799..10236
                     /locus_tag="VEL68_06570"
     CDS             9799..10236
                     /locus_tag="VEL68_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35663.1"
                     /translation="MGEEESKVTKVVREGVVKAYEKGENLIIAVSEITRDVVKKTLAE
                     IQPTKEKVQSLARDAMKGAIQGAKVATVETEEFIKEAAKSIIEEIKWSGTKAADSAKQ
                     AADAILDASKEGGDKAVKEVKDTIKGFVEAWKEVHKKKKPESK"
     gene            10423..10629
                     /locus_tag="VEL68_06575"
     CDS             10423..10629
                     /locus_tag="VEL68_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35664.1"
                     /translation="MKLRTFEGEPVSGELTTEYPVTRNGIPVLLVNGEPFSPKEAEFF
                     LETATQEELGLLKEGGYDLPRWEG"
     gene            10907..>11007
                     /locus_tag="VEL68_06580"
     CDS             10907..>11007
                     /locus_tag="VEL68_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXZ35665.1"
                     /translation="MNIYVGNLSYDLSEEDLRRAFEAFGQVSSATIV"
BASE COUNT         2509 a         2937 c         3098 g         2463 t
ORIGIN      
        1 gagtcgggct tgattccgat caaatagtgg gagggccaca ggggcatcct ggtggagact
       61 gtctggattt ccccgttggg gtccttgaac tccatttccg taaggatctc catgggagtt
      121 tcgaaggagg gaaccttttc cagggccgtg cgggccgacc ccgcccggtc caggacaagg
      181 tcctgggaag ggatcttgaa tcctttccgt gcaacgactg tttcttcctc ctccccctct
      241 tcttcttctt cgaaatcggc gcctctgcgg accagtccct ccttgaccgg gccattgctg
      301 aagacaaagc ggtcaaatcc ctcgaagggg gggatcgaac ggggtcgaac ctcgctgcgc
      361 agtcttaccg ggagcatgcc ggcccctcct ccggccaggt actggacgct caggtcgagg
      421 ggaacctcct tcggtttgac caagggctcc cccgggggct tgatcatccc ccgcaggagg
      481 ggaacccgga actcctccac ccggaaatcc ccggagactc tccgccattc cccgcgccct
      541 tcgccgctcc ccttcttcat caggatcaca ttgtagttgc ccagcttggc atctcgggga
      601 atgacccaag cgctttcggc gattccctgg gcatcccact tcaaggggaa ttcgtacttc
      661 tccccgcttc ccccgtgctg gatggagacc aggtccgggc gcctcgcctc ggccacgggg
      721 gagaagccct gcatggttct ttggcgcagg atgtgcttca tggagacggt ctgtccggcg
      781 cggaaaaggg tgcggtcgaa gaccgtgtgg cccatgaccg gccctcgata atcatcatag
      841 ggaagctgaa acctccaggg ttcgatccct tgattccagc tggagtggac gaaggccata
      901 tcgtcctggg tctgggccat gaccaggaag cccccttgaa gatggagggc ctgggcttgg
      961 gagtaatcat gggaatcgat ggagtgatcg cagcggggaa ggtcctgtcc cgaaggcaac
     1021 ggcccctgga tgcggaacag cccttcggca tcggtcttcc cttcagagag aattttttcc
     1081 tggcagtccc gcaccgtcac ctgggcgtct tttaccggtt cccctttatc cagggtggtc
     1141 acccacacca gggaggattc ccggccccat ttgaaatgga cggagagatt cgtcaccagg
     1201 gctgccgtgg acacatacat cggccggggg gggtcgagca gggcctctcc caggatcgaa
     1261 ctttccagtt ccacaatgta gagccccggc tttttgaagg gaatgcccac cacttcgaag
     1321 gcctggcctc ccccgggttt gggaacctgg aatgaattgg ccgcgttttc atcgccgaga
     1381 agggattttt cccgggaggc cgcggccact tttctgagcc aagcctgaac ctcgaccttt
     1441 ttgtcagggg ggaggctgag gatcttgccg atcaaactgc cgaagattcc cggttcccca
     1501 tcggccttca ccattcgtcc cttgacctgg ggctccagat ttcttagggt gacggggaga
     1561 gcggggtcgg cgttcagctc aaggatgccg aagcgagagg agaatttcgc caggggaggg
     1621 tagcgatcgg ttcgcaccgc gaggggaaat ttatcggcgt tgaccagggg ccggccagcc
     1681 tcatccttca gaccggcggg gagctcgacg cggaaagcgg cgttttccgg gaaaggcccc
     1741 ttgaaggtga tttcgttaaa ataattcgtt tctgcctccg gcaactgggg cttccagacc
     1801 ttttctccgg ggcccttcac gacaatttgc tgagcccatt ttgccggaac aggtgcggaa
     1861 aagtcgaggg acatgggagt gatggggagg caagcgcccc gggggttctc ccgctggcag
     1921 cggaaaacta cggaaaaagc atcccttgtt ttgaagggca gtacctgctc ggtctccgtg
     1981 gccaccccag tcctggtctg aacccctttc ccccagacca ggttgacttt cgccttattg
     2041 gggaatcgct gcctgctttg aatgaggagg aggaaatgcc tggactcttt caaattccgt
     2101 gaaatccagc gggcccttcc ggaatccagg atcttttttc tctcttttcc ctcgaccagc
     2161 gtgatcccca cccgctccgg cagcccttcg acggagaaag tgacgttctt caggatcgat
     2221 tccggttccg gttcggcatc gaggcggagg ataaagacct gctcttcatc gatgctttcg
     2281 ttgccctgat tgggaatgga ttccctcacc gagggcccgc cggtggaaaa ggcgaaagtc
     2341 tctgctcccg tgatctcctt cccggccaaa gttttcaaac cgtgcttcag agtgaattca
     2401 cagcggaccc ccgccggcag gtccttttca aaatcgtaga cccagttctt gggatcggcc
     2461 caacgggggg tccccttctc ggggcaggac acggcgaaag gttcggcaag gtcccggggc
     2521 tcgccgaagg gaaccatggg ttcggaaaac cgtgcgcgga cctgacggat gttcttcacc
     2581 gttccctggg gagtgaaatt ctccacccgg ggggtgtcct gggcccaaag cgtgaggcca
     2641 gggttgaggt gaaggtcaag atggagggtg aggaagaaaa ggaaaaagga caggagagca
     2701 agggaagcct tcttcatatt ttctccttat gggaaaaggt gggcgccttc aacaacgtga
     2761 tatagtatat cccaattcct ttgggactat ccagcgggaa tgcatcgacc ggaggaccgc
     2821 ggcccagacc cgctgaaaaa tcccgcccct ccaaaccgct tttcacttga taaattcgcc
     2881 tatctcccta aaatggtatt tatcgtaagg tggaaggaaa cccatgccct attctaaata
     2941 tcctctgatc agtgctgaag aagcggcttc gaatatccct tccggagcca ctgtcgcctt
     3001 cagcgggttt agcagcgccg gggcggccaa ggcagtcccc cgggccatcg ccaaacaggc
     3061 caggagttta catggaaggg gagaggccta ccgaatccgg gtcttgaccg gcgggtcaac
     3121 cggaaaaagc atcgatgaag aattggctgg ggccgaggcc atttcctggc gtgcccccta
     3181 tcagagcagc cccattctgc gccgtcaaat caaccggggg gaagtggaat atatcgatat
     3241 gcatctatcc catgtgcctc aaatggtggc caatggtttt tttggaaaac tggactttgc
     3301 ggtcatcgaa gcgaccgaaa tcaccccgga cggccgggtt ttcctgacca cctccatcgg
     3361 ctcctcgccc accttcttga aacatgcgga acgggtcatc atcgaaatca acccttacca
     3421 ttcgatgcgc ctgcgggaga tagccgacat tgcgatcatg ccttccccgt cgcaccgcag
     3481 cccgatccag atccacgacc ccctggcccg gatcggccat tcgtatgcgg ccgtcgatcc
     3541 ccggaagatc atcggaattg tcgagaatca agaaccggac ctgatggcct cattcgaacc
     3601 tcccagcgcc tccagcaaaa aaatcgcgga acacataacc cgatttctcc tggaagaaat
     3661 aaggaccggc cgtttgccag agaaactctt gccctttgaa gccgggatcg gcgacatcag
     3721 caacggggtg gtctccacct tgggaaagaa cgaggagatt cctccttttc aaatgtacac
     3781 cgaagtcctc ctcgactccc tcgtatctct aatggagaaa ggaagggtcc tcggggccag
     3841 cgccacggcc ttgaccgttg acccggatac gctgaagggg atctacgaga atatggattt
     3901 ctttgttccc cgcatcctgc tccgttccca ggagttctcc aacaatcctg gaatcatccg
     3961 ccgcctgggg gtgatcgcca tgaataccgc cctggaagtg gacctctacg gaaacaccaa
     4021 ttcgacccac atctgcggga cagacctcat gaacggaatc ggcggtagca tggacttttc
     4081 ccgcaatggc tatatttcct tcatcatgtg tccctctatt tccaagggag ggaaaatctc
     4141 caacgttgtt cccatgtgct cgcacgtgga caatacggaa cattccgttt cgattatcgt
     4201 gaccgatcag ggcctggcgg atatgcgggg actggggccc gctcagcggg ccaaaaacat
     4261 cattgaaaaa tgcgcccacc cggcctatcg cccttacctg catcggtacc tggaaaaagc
     4321 acggaagggt cacctccggc acaacctgca gcagtgcttt gaactgcata acaactttct
     4381 tgaatacggc gccatgcttc ccgatttgga cctcgcggga ctcgagcgcc cataaaaaaa
     4441 ggctccggga tcatggaaaa agtaggggtt tgggcgggga atctatacta ggtctaagaa
     4501 gcccttgggc atccggggtc aatgtgggaa tttttaaccg ttgaagaggc ggcgtcccct
     4561 ggcgaatcaa gggaatatcg atcgccgttc gaggaaaagg attttccatg agcgcaaaaa
     4621 tcaagtctgt ctttgtcttg ggtctcggga aggtcggctt gctggttgcc accctgcttc
     4681 atgaaaccgc ctttcgagtg atgggggccg atcttcggga agcgatgggt ctgccttttc
     4741 ccacggtaaa gctgcatctg agcaatcccg aggagttgaa gaaacggatg gaaggatttg
     4801 atgccgttgt atcctgtctt ccgtacaact ttaacatcgg cgtagcctct gccgcccatg
     4861 agctgggcat gcattatttc gacctgaccg aggacgtgcg cgcagccaat tcgatcctcc
     4921 agatgagtct tacggccagg gcggtcatgg ccccccaatg cggtctcgcg ccgggcttca
     4981 ttgccattgt gggcgcgtcg ctggcggaga ctttcgacgg aatccgcagt atcaaacttc
     5041 gcgtcggggc cctgccccag aatcccacgg gcctgctcgg ttacgccttc aactggtctc
     5101 ccgagggggt agtaaatgag tacctgaatg actgcgaagt gatcgaagac ggtgagatca
     5161 aatgggtgtc ccctttggag tggctggaaa ccatcgtgat tcatggcgta cagctggaag
     5221 ccttcaccac ctccggcgga ctcggcacca tgtgccagac ctacaaggga cgggtcgaaa
     5281 acctcgatta caagaccatc cgataccccg gccacgcaaa gctgatgaat ttctttttcc
     5341 atgagctttt gatgcgcaaa gatcggcaga aggcgggcga aatcctgacc tgcgccaaac
     5401 cgccggtaac cgaagacgtg gtctatattc acgcttcggt tgaaggatgg aagggggggt
     5461 ctctctcccg cgatgaattt gtcagggcct actacccgct ggagattgcg ggccaaaaac
     5521 ggcgcgccat ttcctggacc accgccgctt cggtctgcgc cgtggtggaa atggtgagca
     5581 acgggtcgtt gcccggcaag gggttcctga aacaggagga gattcctttg gccgagttcc
     5641 tcaaaaccaa caacggccgc ctctatgccg gcgaacccct ggggaaaaat ctcgaaggaa
     5701 gaaatcgaca gggcggtgga aaggattgaa gcagcgggca gaaaggtagc ggagcggaaa
     5761 tgagcgagct cttataagga gttgggcttt cttatatccg gttcgatgaa aggagcggga
     5821 tcgatgaatt ccaaagcctg gaaattggga atatgtgttt tggtcgccgt ggtcattctg
     5881 ctgcttccgg ttcccgccgg gttgtcccct gccgcgtggc agctctttgc catttatctc
     5941 gccgccattt tcgggcttgt actccggcct tttgacgaag ccgtcatcct gctcggcgtg
     6001 attgccgcag cgggtttggc cttcgggaat atgggcgccc tgttgagcgg ttacgccagt
     6061 tcgagcgcct ggcttgtttt ctcggccttt atgatcggcg cggctttcag cgccaccggg
     6121 ctcggccggc gcgtggcctt tttcctgatc ggcaagtttg ggcggacgac gctcagactc
     6181 ggctatgtcg ccgcggtgac ggacctggca atcagcccgg cgaccccctc caacacggcc
     6241 cggacgggcg gcatcgttta cccgatcttt cagagcatcg ccatcgccct tgactccaag
     6301 ccggggccaa ccgcgaggcg gattggcgcc tacctgacgc tgaccctgta ttatatcagc
     6361 ttcaccactg ggtatacctt cctcacggcc atggcgccca acgtgctgat cctatccttt
     6421 gccaagagca tcctgaaggt tcaggttgac tggatcctgt gggccaaagc ggcgatcgtc
     6481 cccggcctcg tgaccttgtt ccttatcccg tggcttgtct ataagattta tccgccagag
     6541 atcaagaaga ttgacaacga aaccctgggg aagaaaggcc ttgccgaact gggccccatg
     6601 tccaggcggg aaatgaccct tgctatcctg ttcgtgctgg ccgtattggg gtgggcgctg
     6661 gggtcggtct ttaagattga cgccacggcc gtagccgttg gttttgtcgc ggcctgcctg
     6721 ctgaccgggg tgatgacctg ggagaatgtc atcgcctaca agggggcctg gagcacgttc
     6781 atgtggtacg gcgggatcat cggcctggcg gatgcgttgt ccaagggcaa attttttgaa
     6841 tggctggcga agctgattgc cgcgaatgtg agctttgccg ggatccatcc cctcgccatc
     6901 ctggcggccc tggtcctctt cagcctggcc attcgctatc tgtttgcctc catggcggcc
     6961 tatgtggcga ccatgatccc ggttttattc accatcgccc tggtggccaa ggtcccgcta
     7021 ttgccgctta tcttcctgat cgcctttgcc gcggggtatg gcggcatgct gacccattac
     7081 ggcggcgccc tgagcccggt tctttttgga atggggtata tcgatcaggg gacgtggtgg
     7141 aggaaggggg ccatcatggc cctgatcagt tttgtcacca cctttgtctt cggtttcctc
     7201 tggtggaaca tgatcggact gtggtaatcg cgctggaacg tgcgggtaag gcggagggaa
     7261 atctcaagag aggaaatcaa ctagcggtag agaggattga aacggcaggt gaagaaaggg
     7321 taggctcgcc tgcgatccat ggggagcgga gacgcgggag acaggccgaa attttcaatt
     7381 catccgttcc cctccgttgc ccaaagctat gtcctcagag cggagaatca gcccgaaatt
     7441 tccttcctgg tcatacccca ggcagaagaa ggttccttcg gggagttcca aacgcccctg
     7501 aagctcctgc tccactctca ccttcaaatc gcttaactcc tgccggaagc gatcccattc
     7561 gtattcgttg gagaactgct cgtcaaaatc caccccgaag gcatccgcga tccgctgaac
     7621 ctggtggtat cccgccgggg cgacaacctg aaccatgcag gccggggggg acaggcggtc
     7681 cgggccagcc tgggaaccct tctttctcat tttcaggctg gtcttggcca acttatcccg
     7741 caggtcgtcg atcctccaaa tctggttggc tttcatcatt ctttttttcc tccttcattt
     7801 atttgcttat aaggttagaa taggattgtt atcgattgtt taaggatttg taaatttttt
     7861 ccacggttcg atatcccaga atttcccccc ctttataaaa ggggggcaag gggggatttc
     7921 tgggaggtgc gtggtcatcg gcaaaaatca tgatgcaagc tagccgattt gcattttggt
     7981 tccgccgcgc ggtgtccatc cgtgggtcaa aaaacaaagg gagggtgtat ggaaaggaaa
     8041 gagattctgg ttgatccgaa taaagaggaa tgggaaaaga tcgccttgcc ggggataagg
     8101 attaaatacc tttacaagaa tgagcagact ggggcgtcga tggctttatg ccggttcgag
     8161 aagggttccg gaattcctga gccccacctt cacgcatcca accagttcat gtatgtccta
     8221 aagggaaggt tcacctatcc cggaatcgaa gtgaaagagg ggatgcttta catcaatcca
     8281 aaggacaacg tacatggccc atccctcgcc ctggaggatt ccctggtcct ggagatctac
     8341 gacggtccgc attattaccc ggagaagaag ccgtattaga aagacagcta tcagcggtca
     8401 gctttcagct atcagcgagt agggcgattc atgaatcgcc ccttcagact ttccaggttt
     8461 ggcttttttc ccaaaggatt tggtcaggaa tgcgcataaa ggaggagagg aggtttggtc
     8521 atgagtgcgt tgaaatctga aacggagggg tcaaaaaaac tgatgccggc cctttttgtc
     8581 gggcacggcc atcccatgaa tgcgatccag cagaacacgt tcacggccgc gctttcgaaa
     8641 gcgggtgaaa acctgccccg gccacgggcc attctggtgg tttccgcgca ctggctgact
     8701 cgaaacaacg tgatcgtttc agcgaccgag aggcccagga ccatttacga tttcggcccg
     8761 tttcatcccg acctattcaa aatcgtttat gcccctcccg gggcgccgga acaggcccgc
     8821 cgaaccgccc ggatggtcaa ctccagcaag gtcggggtca ataccgtaag gggattggac
     8881 catggcgcct ggacgattct caaatacctc tttccaacgg ccgatattcc cgtattccag
     8941 ctcagcatcg atttctccca acctccccgc ttccactacg atctcggccg ggagttgctt
     9001 cccttgcgtg ccgaaggagt tctgatcatc ggcagcggca atatcgttca taatctcatg
     9061 cagattacct gggatcatgt ggatgctcct acggagggct gggctaaatc attcgacaga
     9121 accgtggccg agcttctccg aaagggtggt catgacgagt tgattgaata cgaaagactg
     9181 gattatgccg aaaccgccgt acccaccaac gatcattacc tgccgttgct gtacagcatc
     9241 gccctccagg aaaaagggga aaaaatcgaa ttcttttatg aagggtttca gtacgccaac
     9301 atcagcatgc gctgtttccg tataggctga gcttcgttgg gggatgttaa agcaagccat
     9361 caatgtgctc ggtctttccg cctgcagcaa aatccacatc tccacaatcg ccaggccatt
     9421 tccgacctcg aagggggtga tactgttatg gcgactggac agtaaagcca ggtggcaaaa
     9481 cgaaggggag gtgagataaa tactgcaagg agcataaacc gggccgtggt actcggcccc
     9541 tccctcaagc aatcggggag agaaaaatgc tcctcttatg ccttatgaaa agaatgttga
     9601 cttcttaacc gagaggagta tagttttagc agattttggt cctccacctg ctttatttag
     9661 cccggaaccc taaggtgggt ttatgtcctg gagatcacag aaaccccatt tctgaaaaga
     9721 ggtggggttt tttgtttgat gtaggagcaa agaagaaaga gtttcccaat ggcagcaaaa
     9781 tcttgaaggg agtggataat gggagaagaa gaaagcaaag tgaccaaagt cgtaagagag
     9841 ggtgtggtga aggcttacga aaagggtgag aatctaataa tagccgtgag tgagattact
     9901 agggatgtag ttaaaaagac attggcggaa atccagccca cgaaagaaaa ggtgcaatcg
     9961 ctagccaggg acgcgatgaa aggtgccatc cagggtgcca aagtcgctac tgtggaaacc
    10021 gaagagttca taaaggaagc tgcaaagagc ataattgaag agataaaatg gtctgggaca
    10081 aaagctgcag attctgctaa gcaggctgct gatgcaattc tggacgctag taaagagggg
    10141 ggagataaag cagtaaagga ggtaaaagat accataaagg gattcgttga agcatggaaa
    10201 gaagttcaca agaagaaaaa gccggaatcc aagtagaaat cacagtgatg gataccaacc
    10261 ctccgttttt aacaaagtca gttatcccta taaagataga ctcagcccat gccattgccc
    10321 aataccaagt ccatgcaaac tattcaaaaa tttacgccga ggcaggtaaa tagatagaac
    10381 gataaggaga agactctttt ttcctgagga aaggagggtt tcttgaaatt aagaaccttc
    10441 gagggcgaac cggtttcagg tgagttgacc acggaatatc cagtgactcg aaacggaatt
    10501 cccgtcttgc tagtaaacgg tgaacccttc agcccgaaag aggcagagtt ttttctagag
    10561 actgccacgc aagaagagtt gggattgctt aaagagggtg gatatgacct tccgaggtgg
    10621 gagggatgaa gaaaagaaat acgcgaaggc ctgccaaatt caattgggat tattgagata
    10681 taggcaaaca ttacaaacgt ctaggaatcc tgaattagaa cttatccata aatatacttt
    10741 tgcgtttaaa tccccctttg aaaaaggggg acagaggggg atttgctttt aatcgcttta
    10801 aaaaaatccc cccaaccccc ctttgcaaac ggggaaatat tatttatgga caagttattc
    10861 aatattacct aaggagtttc atcgaaaact aaaaaggaga acgaccatga atatttatgt
    10921 tggaaatcta tcctatgatt tatcagagga agatcttcgc cgggcctttg aggccttcgg
    10981 tcaggtaagc tcagcaacaa tcgttaa
//
LOCUS       DASPRR010000170        37881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_197272, whole genome shotgun sequence.
ACCESSION   DASPRR010000170 DASPRR010000000
VERSION     DASPRR010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 37881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37881)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37881
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197272"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VEL68_06585"
     CDS             <1..148
                     /locus_tag="VEL68_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HXZ35666.1"
                     /translation="RGIKDSPPYLHDGRLLTLEDTVEFFNIVLELKLNEQEKKDLVAF
                     MRAL"
     gene            698..2089
                     /locus_tag="VEL68_06590"
     CDS             698..2089
                     /locus_tag="VEL68_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35667.1"
                     /translation="MIPFFVLLILGFLLGAPHAWAESLEERIKKLEETIQKQQEVLKE
                     QQKALEDLKEQSKQAKVAEPLPAAPAPPRDYRLDDRSRGIYVPTASPLTPYSLTKQSA
                     TPGLMNPAISLILDTEYYHSSLSKAELQSRSTPGFIGPQDPPFDEGFNLRSAELAFFA
                     PVDPYFNLYATLPVTENGVELEEAYFVTTSLPAGLQVKGGKFKSGFGRFNAFHPHAWD
                     FVDAPIPYKLFFGGDGEGLIEKGAQLTYLPNLPIYTILGAEVLQGENDTLFGPDGGPH
                     AYTGFVKSSLDFGANQSILFGGSVVGGKTNTTSFAPNTLFQGTSVLYGLEFTYKWKPS
                     RWRSFVLQSEYLYRHQKGDLTDTVALTKDSLKRNQDGFYAQALFQIDRWRIGARFDRM
                     AIFKDEVIRAGEKQNYSGQPYRWTGSLEFNPTEFSRVRLQYNYDQSDPNKLNQEIFLQ
                     FLLAVGAHGAHAY"
     gene            2106..2990
                     /locus_tag="VEL68_06595"
     CDS             2106..2990
                     /locus_tag="VEL68_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HXZ35668.1"
                     /translation="MIRSLILTTVCLLVWASLAAAKLDVVVTLPWIGSLASDIGRDKI
                     KVTTLVKANQDPHYVEAKPSMILDVRRADLLLFNGLELEIGYLPVLVESSRNPKIQPG
                     TPGSFDCSQFVEVIEKPAAVDRSMGDVHPLGNPHYHLSPRNIYRVTGGITELLAAVDP
                     GNVGFYRANLAGWQDRFKEKEKQWAEYPLRGKKFLPYHKFFEYLAHEFGFEILGYVEP
                     KPGIPPSAGWVEKIIELTKRVKPDAILTTSYYGTREVTFLSQKTGVKYIVVPHDVGAT
                     PACKDWFSLMDQVLAELK"
     gene            3000..3806
                     /locus_tag="VEL68_06600"
     CDS             3000..3806
                     /locus_tag="VEL68_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXZ35669.1"
                     /translation="MEAFHFLIVPFVGCVVLILIHAYFGIHILERGVIFLDLSLAQFI
                     AVGIAVSCFTGDVPEHRFFYAAAFAVFGASILSFSKQIARLVNIEAFIGVLYVFSFAA
                     SILFLDKSAHGTEEFKSILNGNILWMTRRDLLMASLLYAGIGAFHLIFWKKFLALSYG
                     GGKGFLWEFFFFLSFAFVLVSSVQIAGILQVFSFLVIPALIGRLYNRNPIQILLIGWG
                     IGIVVCVIGVSLSYKLDVPTAPLIVASLSLIFFVLLGVKARKSGAEVEKR"
     gene            complement(3893..4312)
                     /gene="nikR"
                     /locus_tag="VEL68_06605"
     CDS             complement(3893..4312)
                     /gene="nikR"
                     /locus_tag="VEL68_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HXZ35670.1"
                     /translation="MAGLIRFGVSIPDSLLGDFDRLIAQKGYQNRSEAIRDLIRDSLV
                     KEEWREGKQEMVGTVALVYSHHTRELSRILTKMQHDHYKTILSTLHIHLDEHNCLEVL
                     VVKGMGGDIKKISDRLIGIRGVKHGKLSLTTTGKNLR"
     gene            4829..5809
                     /locus_tag="VEL68_06610"
     CDS             4829..5809
                     /locus_tag="VEL68_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXZ35671.1"
                     /translation="MKEILRHAREVIQIEAEAVQGLLDRLDDNFARAVQLLLACEGRA
                     VITGIGKSGLVGRKMVATLTSTGTPATFLHPVEGLHGDLGMVTAKDVVVAISNSGETS
                     EIIHLLPSLQKIGAKIIGLTGSLDSTLARSSHIVIDVGVEREACPLGLAPTSSTTATL
                     VIGDALAVALMRQRKFGEKDFALFHPGGSLGEKLSFKIKDIMRPAADFPSLSSDKTVG
                     EFISAINRCGADFALILQANGGLAGMVSDRDLRRGEIGSPDFLNRPIKKIMDPHPPTT
                     EEEVSAAEAMRTMVEKNLVALPVLDKKKNLKGVVTLRDLLNRRDGGDVVK"
     gene            5806..6756
                     /locus_tag="VEL68_06615"
     CDS             5806..6756
                     /locus_tag="VEL68_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXZ35672.1"
                     /translation="MNPYPPKGLIVALITPLNEEGQVERVSLHRLIDRVVPYCDALMI
                     GEGLIGEGLSLPNPSRLELLQVSAEMVAGKKPLFLCPTAGTTGETVNNITVLTQALKN
                     YPDQDSLFWVDIPLWYHSNRNLPQFYQEWREYTPYPILLHNHPLLIGKRNRSLKRTNI
                     RTAVLKLLAENGQIVGLIQSGDLKRTIHYQRAVRARRDFRFFDGDEGNFLNSPSSSGV
                     VSGGANLFPSEWSEVVTASLKMSEDPAKNLLLLKRSQKLRELSQVMQKNPAQSLKVAL
                     HRLGWLSGAKGLDFTQENSLEAVEGLISFLQVHFSLQTPS"
     gene            7018..7329
                     /locus_tag="VEL68_06620"
     CDS             7018..7329
                     /locus_tag="VEL68_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35673.1"
                     /translation="MKKIDPAYLSADEKSVLENFGNPDYIRFYRKRSLERERVYAWVY
                     ADPVRFVTFIDGHKVDYAVLDEDLSSLNEYQRNMLFWGGITAGTLAALGLLYYFLFAK
                     K"
     gene            7345..8082
                     /locus_tag="VEL68_06625"
     CDS             7345..8082
                     /locus_tag="VEL68_06625"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXZ35674.1"
                     /translation="MIKLVLKLKETQIEEFALEKDEINIGRAKENDIVIDNIAVSRKH
                     ALIQRKVGENYVIRDLNSSNGTFLDGIQIDADDHTLTDGAVIGIAKFEIIVKGLSKQV
                     PPPTPKPPASEDVEGTMIFDAARRKPTPEVQAPSEPKPIRWPALSATKGPSKGKEYKI
                     TKEVTLVGKDPQNDIPAEGWFVSSPHAKIFRRGDRFYISHLGGFLSTTKVNGIGIRED
                     HILKNKDQVDIGNCTFVFTQAPAEHTD"
     gene            8120..8881
                     /locus_tag="VEL68_06630"
     CDS             8120..8881
                     /locus_tag="VEL68_06630"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HXZ35675.1"
                     /translation="MKIRYAAASDRGRVRKNNEDAFLADPGLGFFAVADGMGGHAAGE
                     IASRLALDTLRKSIAESKQNRETEFSPNQTALLSPPSTLLVNGIRLANQAVFQSSQEK
                     EEYRGMGTTVVALFLFDSSSVVAHVGDSRLYRIRGGRIDQVTEDHSLVWEQYREGSLS
                     KEALSSSPLKNIVTRALGMNLTVDVDVQEIDLQKGDLLVLCSDGLSDLVQDEELMSTI
                     RRASGDLNRACHDLTQLANQRGGKDNITTLIIQIE"
     gene            complement(8946..10319)
                     /locus_tag="VEL68_06635"
     CDS             complement(8946..10319)
                     /locus_tag="VEL68_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HXZ35676.1"
                     /translation="MKRSLDPLRRDAKKIFLHAIHAVQPQTILSSHISLRNHVLRIGE
                     RKYPLSRYEKIFVAGTGKASASMAVNLEKILGTRITRGLINVKYGHSQKLKRIRVQEA
                     GHPLPDEKGLEGAKEMVEMLSHLTGNDLVIFLVSGGGSALLPLPVEGVTLVEKQATTN
                     ELLSCGASIQEINTLRKHLSRIKGGGLAKIVYPATLVSLILSDVIGDPLDAIASGPTV
                     PDSTTYENCAWILNKYELWGKIPPSVASHIRAGMEGRKEETLKEGDPAFAKVYNLIVG
                     NNLLAMTAARKAAQALGYRTLLLSSLVEGETREIAKVHAAIAKEVLLSGNPIPPPACI
                     LSGGETTVTLKGKGKGGRNQEFALAGALEIAGCEEILMMSAGTDGTDGPTDAAGAFAD
                     GKTVIRAKTMGLDPWSFLKENDSYSFFQRIGDLLTIGPTGTNVMDLRIMLVTPPNSPK
                     GTRTQRI"
     gene            complement(10351..12180)
                     /gene="glmS"
                     /locus_tag="VEL68_06640"
     CDS             complement(10351..12180)
                     /gene="glmS"
                     /locus_tag="VEL68_06640"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXZ35677.1"
                     /translation="MCGIIGYIGPRETKEVLLEGLRRLEYRGYDSAGVALFHEGKVVI
                     RRAMGKLKELERKTEGEKFNGNLGIGHTRWATHGKPSESNAHPHKAGGVAVVHNGIIE
                     NYLALKNILAGEGHTFKSETDTEILCHLIQRYIFKGEKLEEAVRQALLQVHGSYAIVV
                     LYEGEKDKMIAARKESPLILGLGDKDHFVASDIPAILPYTRKMIFLEDGEMAVVQEKR
                     YRISTLWGENRQRPAKEILWDPVTAEKGGYKHFMLKEIFEQPRAIVDTVRGRVSLEEG
                     KVFLEGAGLNSKALKDIRRVCIVACGTSYHAALVGKFMIEGLSRIPTEVDLGSEFRYR
                     DPIVGSQDLLLAISQSGETTDTLAAFREGKRKGMRTLSICNVVDSSLARESEGVFYTH
                     AGPEIGVASTKAFTTQLVGLFLLALYLAENRETLGRQTIKDLLEELLKVPHYLQQTLE
                     LNTQIEALSKKYMNARDFLYLGRGVNYPIALEGALKLKEISYIHAEGYPAGEMKHGPI
                     ALIDEDMPVVVLAPMGRTQEKILSNIEEVRARDGKVIALTNPSCQEMVSKARDTLLVP
                     ETHPFLAPILLTVPLQLLAYNVAVLKGTDVDQPRNLAKSVTVE"
     gene            complement(12373..13734)
                     /gene="glmU"
                     /locus_tag="VEL68_06645"
     CDS             complement(12373..13734)
                     /gene="glmU"
                     /locus_tag="VEL68_06645"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXZ35678.1"
                     /translation="MSELAVVILAAGKGTRMKSDLVKVLHSVAGIPMLSYPLDVARSL
                     KPSRLVAVVGFQADLVQAKFKADDVTFAIQKEQLGTGHAVLATRAALQGFHGNVLILS
                     GDVPLLTSETVGKFLKPHREHQAALSVMTVELENPKGYGRVFRNGDGSLLRITEDKDL
                     KPGEEKVREINTGIYCVDADFLFSALSQVTPQNAQQEYYLTDIVARANSEKKKVLPFL
                     VENSLEVMGINTRVELAKANHYMRSLIAERHMLEGVTLIGPETTYIDREVKIGRDTVI
                     YPNCYLLGKTSLGIACLVEPGCKLSDTQAGNHVTIKASSVIAESILEDHVEIGPFAHL
                     RPQNVLREGSKIGNFVEVKKSVIGKGTKANHLSYIGDATLGEKINVGAGTITCNYDGK
                     KKHATVIEDGVFIGSNTSLVAPIKVSRNSVIGAGSTITKEVPPDTLAVARGKQVHYRR
                     RSK"
     gene            complement(13863..14045)
                     /locus_tag="VEL68_06650"
     CDS             complement(13863..14045)
                     /locus_tag="VEL68_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35679.1"
                     /translation="MPWDPLAAKDQVLEMGKFPRERAGPESMNPGHAVKKDRECMFEY
                     LQEEKDKVKKNSQIDD"
     gene            14090..15688
                     /locus_tag="VEL68_06655"
     CDS             14090..15688
                     /locus_tag="VEL68_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ35680.1"
                     /translation="MEFSSLVATLQNPQIYPEKPAEIELVQTHISAIFFAGEHVYKIK
                     KPVNLGFLDFTSLEKRKYYCQQEVDLNRRLAKEVYLGVVEVRSHQGQISIGESPGKVV
                     EYAVKMKRLPQDCMMDQWLAKGAVTPKIVERIAAKIAHFHALAATSPEISKFGNIQTI
                     RGNLEENFYQTEKYVGRALTSNLYEEIMEATRRFMDSHTPLFYKRIADGMIRDCHGDL
                     RLQHICMGEDILIFDCIEFNHRFRYSDVVADLAFLLMDLDYHKQPLISADLASHYLTI
                     SRDWPLYLLLDFYKSYRAYVRAKVTSFRLDDPNISTETRSSTLKEARRYYLLSHYYAK
                     RMNHLLLVVTGGWIGTGKSTIARALAEALGWEWLRTDVLRKELAQVSPLEHRFEEFHQ
                     GVYSPDFSQKTYQNLFERARNHLESGKSVILDASFKKQADRQMARDLARNTQADFLLI
                     ECQCSDEEIKRRLARRAWEKSEPSDGRWELLAEQKKDYDPVEGFDPDLIIFLNTERPL
                     EECLAQIFRHLFMRAGRELSHAVH"
     gene            15745..16506
                     /locus_tag="VEL68_06660"
     CDS             15745..16506
                     /locus_tag="VEL68_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ35681.1"
                     /translation="MTRWRFFLFLLAGLSLLSGCPPDPKAVQKASYHFGLGTSYLQQG
                     DPTSALRELYEAEKLNPQDAQVQHYLGMALSAKGKYTDALEHYRKALELNPKYTEVHN
                     TMGATFLEMGKWEDAIREFKLVLNDILYLTPFFVYNNLGWAYYKKGDLNSAIENYRKA
                     LGMKPDFGLAHYNLGLAYRDRKQPNLAIGSLRQATVLVPGLLDAHFQLGKLYFETGKN
                     EEAEKSFQEVVRLAPQSESAQMARQYLDLLKKSRK"
     gene            complement(16507..17637)
                     /locus_tag="VEL68_06665"
     CDS             complement(16507..17637)
                     /locus_tag="VEL68_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HXZ35682.1"
                     /translation="MIIDGLSSLLAGFSNFQWSHGVMIVVGCALLYLGIKKDMEPLLL
                     VPIGFGAILVNIPTATLMEEGGLLRHFYDAGVLTEIFPCLIFVGIGAMTDFGPLLENP
                     RILLLGGAGQFGIFLTLLLALFLGFTQLEAVAIGIIGACDGPTAIYVTSKYAPHMLGA
                     VSVAAYCYMSMVPIIQPPIMRALTSKEERKIVMKYQKKTVTPAVKILFPIAVTLVTCL
                     IAPMGTPLMGTLMLGNLLKESGVTDRLVKASQNEIANIVTLLLGISIGATMSGPVFLK
                     PQTLIVLGLGLVAISLDTAMGIFLAKIMKVLSGGKVNPLIGAAGISAFPMSARVVQVE
                     GQKANRKSYLLMHAMGANTGGQIGSVLAAAVMLSLLKGMGIV"
     gene            complement(17640..17783)
                     /locus_tag="VEL68_06670"
     CDS             complement(17640..17783)
                     /locus_tag="VEL68_06670"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HXZ35683.1"
                     /translation="MQNLEFGLTLTVLGMGGTLVILFLIGLVVDGMNRILLWGERKKE
                     GRG"
     gene            complement(17876..18868)
                     /locus_tag="VEL68_06675"
     CDS             complement(17876..18868)
                     /locus_tag="VEL68_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35684.1"
                     /translation="MRIKIFTARDIDQALSMTEAVEVVKDAFVQLSLKKAQSPVRTSL
                     NLKEPGEAALVMPAYLEESRALGAKMVTVLPWNPRAHLPAVQAIVFLFDAATGSPLAV
                     LEGTHLTRFRTGAATGAATQVLSRADSRSLAVFGAGGQSFFQVKGVLAARKIERIKVF
                     DLLPERTDGLIAALGADASCRGVELLRAGTPTQMLEGTDIVVTATTSREPVFDGRQLR
                     EGMHINAIGAFKPEMQEVDEITVCRSRIFVDSLEACLEEAGDLIIPLTRGLIQETDIL
                     AELGEVAAGRKAGRGSAREITYFKSVGNAVQDVSVAQAVYRRAQKKNLGQEVEL"
     gene            complement(18865..20124)
                     /locus_tag="VEL68_06680"
     CDS             complement(18865..20124)
                     /locus_tag="VEL68_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXZ35685.1"
                     /translation="MDFSKIHNYLREQRKKAEESDLPLKSVRVIDLGAVVAAPFGATL
                     LGDFGAEVIKIEPPDVPDAIRYWARVNNDMEPFWLVASRNKLPITLNLKHPEAKKILV
                     QLVEKSDVLFENMRPGTLDRLGFSAEQLWKINKGLIIGRISGYGQSGPYRNKPGFGTL
                     AEGMSGYTYMNTHPGAPPTNPPIALADMIAGTHLALGAMVALRDQKRGERGGKEFEVS
                     LYEPLLGYMGGQFLIYSLTGDNPQPMGNESNFTSPRNSFQTKEGKWVALSASAQAPFE
                     RMMDLMGHSELKTTAGFRNNQERLQNESRAVLNRIIGDWIRARTLKGVLEECEKAGVT
                     IGPVNNMEDIANDPHVQQRGSLASVYDPASGKTLSFPEAPVRFLKTPGQVRFPGLPMG
                     SANEVVLVDLLGYSSAEIKELKAKGAI"
     gene            complement(20558..20633)
                     /locus_tag="VEL68_06685"
     tRNA            complement(20558..20633)
                     /locus_tag="VEL68_06685"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20597..20599),aa:Glu,seq:ctc)
     gene            20955..21746
                     /locus_tag="VEL68_06690"
     CDS             20955..21746
                     /locus_tag="VEL68_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXZ35686.1"
                     /translation="MDYRSILYQKRGGKAIITLNRPRALNALTQETIEELLNAIQEAS
                     LDPEVRVLILTGAGRAFCFGADISEFSRQIATGTPDSGILLLRKAQEVIRLLTSIPKP
                     TIAALNGFATGLGLDLALACDLRVAAQRAKLAEAFITMGLIPDGGGTFTLPRLVGLAK
                     AAEMIFIGEPLNASEAERIGLVNRVVPTEELAKATENLADKLAKSPCLALGRAKQALW
                     KNLAGDLASALKVEAQIQLTCLASDDHREAVQAFLEKREPVFRGT"
     gene            22384..23427
                     /locus_tag="VEL68_06695"
     CDS             22384..23427
                     /locus_tag="VEL68_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXZ35687.1"
                     /translation="MTQYEMESISPSSSSPVIGLNGKLPEGETRTTGISAAFPTLLND
                     RVKTYIDLFQNKADHFFTRSLARSQAYEGIIRPILREKNLPEELFYLALIESGYNPYA
                     QSRAKATGIWQFMKGTAKRFGLRVDQWVDERRDPEKSTLAAADYLKSLYEMFNCWYLA
                     AAGYNAGEAKILQAMKRAKSQDFWAISQQRYLKRETKEYVPRLLAAMIIAQDPAKYGF
                     SNIDYHPPLVYEKVVVPSGVRLDRIARAAETTVEEVRALNPALRRDKIPPHDPSFEIK
                     LPPGKKEAFEKNFSQVFKLDPRTARKHLVRQGETLGQIAKKYKVDLRELCEGNEINPK
                     TLIKPGMTLLLPP"
     gene            23442..24683
                     /gene="glp"
                     /locus_tag="VEL68_06700"
     CDS             23442..24683
                     /gene="glp"
                     /locus_tag="VEL68_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HXZ35688.1"
                     /translation="MILVEEALHHILGKISRLGSERIPILQALGRVLAENIFASRNIP
                     AWDNSAMDGYAVRAEDILSTSKEKPVVLRVLGDLPAGKVFKGRGGSGEAVRIMTGAPL
                     PPGFDTVVPVEDTEKDGERVRILETPGKGKNIRLSGEDVKSGERILEEGTLLRPAHIG
                     MLASIQRSMVSVYQQPRVAVLSTGDELLEIDEPWEDGKIVNSNSYSLAALVAESGGIP
                     IQLGIAKDRREELTAKIRQGLVADLLLTSGGVSVGDYDLVKEMLNELGQMNFWKVAMR
                     PGQPLAFGMISEKPLFGLPGNPVSSMVSFEQFVRPSIRKMSGYKDLFRPVLQAILRED
                     ISKKAGLAHFIRCRLILEKGKVYATTTGEQGSGILSSMVKAQGLIVLPREETLARVGE
                     AVKVILLDPSFFHLSGPDFLS"
     gene            24853..25275
                     /gene="rpsF"
                     /locus_tag="VEL68_06705"
     CDS             24853..25275
                     /gene="rpsF"
                     /locus_tag="VEL68_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXZ35689.1"
                     /translation="MRRYETIFITHPELTEEELSELKEKILSILTQFKGDLIKLDDWG
                     IKKLTFEVRRNSRGRYFLIDYAAGKDLIRELERNLRLNDRVLRFQTVRTSDELTPEAI
                     KTLKESAGPEKAKGSERIEPPTGIKGGDEAQVEGGEKE"
     gene            25272..25520
                     /gene="rpsR"
                     /locus_tag="VEL68_06710"
     CDS             25272..25520
                     /gene="rpsR"
                     /locus_tag="VEL68_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXZ35690.1"
                     /translation="MKPPATRKKRPFQRRKVCVFCADSGIKIDYKDTKMLTHFITERG
                     KILPRRISGNCAKHQRELTTAIKRARNIALLPFTVIST"
     gene            25659..26126
                     /gene="rplI"
                     /locus_tag="VEL68_06715"
     CDS             25659..26126
                     /gene="rplI"
                     /locus_tag="VEL68_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXZ35691.1"
                     /translation="MKVILIENVPSLGKPGDTVQVAEGYGRNFLIPKKMALEATPANL
                     HRLEQQREVFLQKGAKEKQSASDLAAKLDELSCTLARPAGENEKLFGSVTSMDLQRFL
                     QEKGISLDRRKILLPQPIKTLGSYSVPLKLHPEITAQLKVNVVAASPEEKEKP"
     gene            26155..27501
                     /gene="dnaB"
                     /locus_tag="VEL68_06720"
     CDS             26155..27501
                     /gene="dnaB"
                     /locus_tag="VEL68_06720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXZ35692.1"
                     /translation="MDLLAQRLPPQNLEAEISVLGGVLLDNEALNRVLEVIKEGDFYR
                     ESHRKIFSAILDLYERSEPVDLITLAEALRKREVLEEVGGIEYLNSLANSIPTAANIT
                     YYAKIVKEKATLRKLVNRATEIVSQVYGHSGDVDDFLDQAERSIFEISEDRVRPSFYP
                     IKDIIKSSFKTIERLYEKRQLITGVPTGFAKLDELTSGFQPSDLIIVAGRPSMGKTAF
                     ALNITAHAAIEAAVPSAIFSLEMAKEQLALRMLCSDAKVDAHRLRGGFLSESDWPKLT
                     RAAGSLSEAPIFIDDTPGLTALEMRAKTRRLKAEHNVGLVVVDYLQLMRGRADSDTRE
                     QEISDISRSLKALAKELNLPVIALSQLNRRVEERGDRRPQLADLRESGAIEQDADVII
                     FLYRDEVYNKSEDNPNKGKAEIIIGKQRNGPTDKFELTFLDKFTCFEDITYRQEAR"
     gene            27604..28938
                     /gene="dnaA"
                     /locus_tag="VEL68_06725"
     CDS             27604..28938
                     /gene="dnaA"
                     /locus_tag="VEL68_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXZ35693.1"
                     /translation="MKELWNRVLADVGEKISQKAYDIWLKPLSLISVKETEFFLEVPN
                     KFFKDWISENYQNLIKESLSHLTGQQYTIQFIIKENAEEKPQNPKDEERISIPRPARQ
                     AVREDGLNPNYTFDDFIVGSSNQFAHAAALAVANLPAKNYNPLFIYGGVGLGKTHLLN
                     AIGNQIAETDPSANICYTTSEKFTNELINCLRYEKMTDFRNKYRNKDALLLDDIQFLG
                     GKERTQEEFFHTFNSLYESHRQIVITSDKLPKDIGGLEERLRSRISWGLIADIQPPDI
                     ETKVAILCKKAELFNISLSSEVGLFMASNLGNNIRELEGALTRLRAYSSLTGSEISIS
                     MAKETLKDILSDRQKMITIDSIQKAAAATFNISVSDLKSSKRLKIYSIPRQISMYLCR
                     TMTKCSYPEIGEKFGGKDHSTVIHAVRQVEKRLNEDRDLSNLIEKLKNQLHK"
     gene            29159..30274
                     /gene="dnaN"
                     /locus_tag="VEL68_06730"
     CDS             29159..30274
                     /gene="dnaN"
                     /locus_tag="VEL68_06730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXZ35694.1"
                     /translation="MEFLIEKGEFTKGLFRSQSVVEKKGTMPILLNVLIETKDNGIFI
                     TATDLEIGIKGQYQGDVQKTGNITVSAKKLYEVVKELPEKNVSFRMKENQWVEIKSGK
                     SVFNIMGVSAETFPSFPAYEEEEFMAMEASVLREMIDKTIFAVSTDESRYNLTGVFLA
                     KHKEEEDQKMRMVATDGYRLSLIDRNMKTEIPGLEKGVLLPKKGLAELSRLLEEGGDQ
                     VWIKLKNNNLIIKRENVVLIMRLLDAEFPDYNQVIPNQTKRHIRIKRSPFIDSLKRVS
                     ILSSEKTKGVKFHFGENLLELSSYNPDFGEAKEEISVEYRGEELTVGFNYRFVLEVLN
                     ILSSEEIIMEFEDGVSPAVIRPANDEKHTCVVMPMRI"
     gene            30290..32743
                     /gene="gyrB"
                     /locus_tag="VEL68_06735"
     CDS             30290..32743
                     /gene="gyrB"
                     /locus_tag="VEL68_06735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXZ35695.1"
                     /translation="MAKKNDKEIIQSGGPKEYTAENIKVLQGLEAVRKRPAMYIGSTG
                     IAGLHHLVYEVVDNSIDEALGGYCNKIDVVIHEDKSVTVEDNGRGIPVDLHKEQGKSA
                     AEVVMTTLHSGGKFDSDSYQYSGGLHGVGVSVVNALSEFLEMEIYRDGKVYSQRYERG
                     VPVTPFETIGNTKRRGTKIKFRPDTEVFETLDFSYDTLSQRLRELSFLNSGLKITVVD
                     ERTDKKQEFQYKGGIVSFVEYLNKNKTTIHPKPIRLEGEKEGVYIDIALQYNDGYTEN
                     LFSFANNINTVDGGTHLIGFKSALTRTLNNYGANLSQFKNLKASLSGDDAREGLTGVI
                     SVKLSNPQFEGQTKAKLGNSEIKGLVEALVNEKLATYFEESPSVAKRILEKVIDAARA
                     REAARKARDLTRRKGLLESDSLPGKLADCQEKDPRESELFIVEGDSAGGSAKQGRDRK
                     IQAILPLRGKILNVEKARFDQVLSNQEIKTLITALGTGVGEEEYNLDKLRYHRVIIMT
                     DADVDGSHIRTLLLTLFYRQMFPLIERGHLYIAQPPLYRVKKGKEEPKYLRSEEDLED
                     YLLEKGIENMRLLLPEDKGQLSGKGLLEMVKKAIRLDKILHRLAKRKMDPDILLAFAQ
                     QEAFSKNTLKSEKEINRVIEQVKKALKKEKKNKGEMEFQMEKDPEHGSYNLRCVTSTN
                     GSRLETSLDLELVSSGQFEELRKLSKQLSSLGPAPFVLKTGESSQEVAGLSPLMQEVF
                     SGGKKGLEIQRYKGLGEMNPEQLWETTMNPETRTLLQVRLEDAVKADEIFTVLMGDQV
                     EPRRDFIQRNALSVTNLDI"
     gene            32847..35351
                     /gene="gyrA"
                     /locus_tag="VEL68_06740"
     CDS             32847..35351
                     /gene="gyrA"
                     /locus_tag="VEL68_06740"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXZ35696.1"
                     /translation="MASHPGLNQLPVKLEEEMQKSYMDYAMSVIIGRALPDARDGLKP
                     VHRRVLYAMHELSNDWNKPFKKSARIVGDVIGKFHPHGDVAVYDTLVRMAQDFSLRYP
                     LVDGQGNFGSVDGDPPAAMRYTEVRMARLAHELLSDIDKQTVDFTPNYDGSLNEPAIL
                     PAKIPNLLINGSSGIAVGMATNIPPHNLKEVIEATIAVIQNPDISVEELIQLVPGPDF
                     PTGGFILGRGGVREAYLTGRGMVQMRARALIERQKRTERESIVITEIPYQVNKARLLE
                     RIAELVQDKRVEGIADLRDESDREGMRVVIELKRDAVAQVILNQLFKHTQMQATFGVT
                     LLAIVRGQPQLLNLKELVEQFILHRKEVVTRRSQFELGKAEAQAHILEGLKIALDNLD
                     RVIALIRASKTPKDAREALMAKFSLSEIQAQAILDMRLQRLTSLEREKINEEYAEVIK
                     LIARLKEILGNERILLNVIVEELQEVRERYGDERRTEIIEEVQEIKLEDLIVEEDMVV
                     TISHTGYIKRNAISLYRSQRRGGRGKMGMTTKEEDFVEHLFIASTLSYILFFTDAGKV
                     YWLKVHEVPQAGRMTRGKAIVNLLNLSGNEKITAILPVRAFEEGKYVLMATKQGIIKK
                     TDLMSYSHPRVGGIIALSLDEKDQLISACLTDGTKEVLLSSREGKAIHFVEGDVRDMG
                     RAARGVKGITLGKDDFLVSMDILSPGAVGSSILSATENGFGKRSPIEDYRLQSRGGKG
                     IITVKTTARNGKLVGTQQITDQDEVMLITDRGKIIRLRCGEIRLSSRNTQGVKLIELE
                     SGERVMAVARLAEREEEGNGQEGEEVPEEPCPEPES"
     gene            35355..36398
                     /locus_tag="VEL68_06745"
     CDS             35355..36398
                     /locus_tag="VEL68_06745"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXZ35697.1"
                     /translation="MNRGRRRIAVIGAGSWGTTLANLLAEKEEEVLLWVYEEDLFRVL
                     SAKRENDFFLPGVRLADSLRFSHSLEEALAERETVVCAVPSHAVREVFLRARPHLSRK
                     ALLISVTKGLEDGTHLPMSRVLQEVMGSGTKAEIAALSGPSFAGEVSRRIPTAVAAAG
                     TTFRAAQKAQDLFFRPYFRVYTNPDLIGVQLGGAIKNVMAIAAGASDGMGFGHSSRAA
                     LITRGLAEMTRLGVSMGAQRETFFGLAGLGDLVLTCTGDLSRNRQVGLELGRGKALQE
                     ILGGMRMVAEGVRTAKALWELARIQKVEMPIAQNVYEILYQEKGPREAVQELMSRLPR
                     SEQEDLPVDSSHF"
     gene            complement(36432..37082)
                     /locus_tag="VEL68_06750"
     CDS             complement(36432..37082)
                     /locus_tag="VEL68_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35698.1"
                     /translation="MRELLRLQKYSAPVLAAIFLILLSACLYRVNLEPPVSPKVDLAE
                     YQPLALLPIQDATGHPASGSNLYPIIRDSLEKKGYHLVEEIKVVSTLEEMRLTALLIL
                     SDRDSLKKVAERLKARLIMIASLPEYRVQKSRLGSQSVQSWDGESFTDQVLPTYFGGS
                     SQVRLILRIFESEKGELVWMSAGTIHASRDSAEIYGRKLAERLLISLPPVSPPSTK"
     gene            37319..37681
                     /locus_tag="VEL68_06755"
     CDS             37319..37681
                     /locus_tag="VEL68_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXZ35699.1"
                     /translation="MPIYEYRCQECGKVSSYLVMNIREAHHPQCKRCQSGKMTRLISR
                     VARIRSEESRLESMADPSKLGGLDENDPASMARWMKRMGKEMGEDLGEDVDSMIDQAM
                     EEEMAGKGAESGSKDEEL"
BASE COUNT         8945 a        10444 c        10152 g         8340 t
ORIGIN      
        1 ccggggtata aaggattctc caccctacct tcatgatggg cggctgttga ccctggagga
       61 tacggtggag ttcttcaata tcgtcctcga actcaagctc aacgagcagg agaaaaaaga
      121 ccttgtggcc tttatgaggg ctctctaagc gctcctcctc gccctctggc cgttcgaggg
      181 cgaggggacc ttccggtccc cctgagaagt ttcttctgct ggggaaaggg acaggcaacc
      241 cgcagggagg tttcggccaa ggcttgctag ggataattca atggagcgga agtccttctt
      301 attcctttcc ctttcctttg ccttctttct tctggcgggg gccttccacc accatccgga
      361 tgggtgtaaa catgaggctt gctccctctg ccatttgacc tccacccact cggattactc
      421 cggcacggcg ggagcttcca ttgctccccc gatgtcctca tcaatttccc ttccgcccgg
      481 tgcctcctcc tttcctctat ccttcttttc ttttttccgc ttcggccggt cccctcccca
      541 atccctttct ctttcctgac ctttcaaaag gcggctggct gcctgcgggc ctccgagtag
      601 ccttcatacg aattcatgca acatcgaagg tgccagagag cctggccggc taagccggaa
      661 gggttaaaaa tacatctcga tttcgaaagg aggagaaatg atcccattct tcgtcttatt
      721 aattctgggt ttcctcctgg gggctcccca cgcttgggcc gagagcctgg aggagcgcat
      781 caagaaattg gaagagacca ttcagaagca gcaggaggtt ttgaaagaac agcaaaaagc
      841 tctggaggat ttgaaggagc agtccaaaca ggcgaaggtt gccgaaccgc tcccagccgc
      901 accggctccg ccgcgcgact accgcctcga cgaccgttcc cgggggattt acgtgcccac
      961 ggcctctcct ttaacccctt attctttgac caaacagtcg gccactccgg ggctgatgaa
     1021 ccccgccatc agcctcatcc tggataccga atactaccat tccagcctgt caaaggcaga
     1081 actgcaatcg agaagcacgc cggggttcat cggccctcag gacccgcctt ttgacgaggg
     1141 attcaacctg cggtcggcag aactggcttt ctttgccccc gttgacccct acttcaatct
     1201 ttacgctacc cttccggtca ccgagaacgg cgttgagctg gaggaagcct attttgtgac
     1261 cacttccctc cccgcagggt tgcaggtgaa gggcgggaaa tttaagagcg ggtttgggcg
     1321 cttcaatgcc ttccatcccc atgcctggga tttcgtggat gctcccattc cctacaaact
     1381 cttcttcggc ggcgatggag aagggctgat cgaaaaagga gcgcagttga cctacctgcc
     1441 gaaccttccc atttatacca tcctgggggc ggaagtcttg cagggagaga atgatactct
     1501 cttcggccca gatgggggtc cccatgcgta taccgggttc gtcaaatctt cgcttgattt
     1561 tggcgctaac caatccattc tgttcggagg ttcagtagtc ggaggcaaaa cgaacacgac
     1621 ttcctttgcc cccaataccc tgttccaggg caccagcgtt ctgtacgggc tggaattcac
     1681 ttataaatgg aagccttcca ggtggcggag ctttgtcctg cagtctgaat atctgtaccg
     1741 ccatcagaag ggtgatctga cggatacggt cgccttgacg aaggattcat tgaaaaggaa
     1801 tcaggatggt ttctatgcgc aggccctttt tcagatcgat cgatggagaa tcggggccag
     1861 gttcgaccgc atggccatct ttaaagacga agtaatccgg gccggtgaaa agcagaatta
     1921 ttccggacag ccctatcgct ggactggttc tctggaattc aaccctacgg aattttcccg
     1981 ggtccgactt cagtataatt acgaccagtc agatccgaac aaactgaatc aggaaatctt
     2041 tttgcagttc cttctggccg tgggagctca cggagcccat gcttattgag aaagggaggt
     2101 cgggaatgat tcgaagttta atcctgacaa cggtctgcct gttggtttgg gccagtttgg
     2161 ccgcggccaa gttggacgtc gtcgtaacgc tgccgtggat cggaagcctg gccagcgata
     2221 tcgggcggga taaaatcaag gtcaccactc tggtgaaggc caatcaagac ccccattacg
     2281 tggaggccaa gccgagcatg attctcgatg tgcgccgggc cgacctgctg ctgttcaacg
     2341 gtctggagct cgagattggg tatctgccgg ttctggtaga atcttcgcgg aatccgaaaa
     2401 ttcagcccgg gactccgggt tccttcgact gttcccagtt cgtcgaagtc atcgaaaagc
     2461 cggcggccgt cgatcggagc atgggagatg tgcatcccct tgggaatccc cactaccacc
     2521 tctccccccg gaatatttat agggttacgg gggggatcac cgagcttctg gcagccgtcg
     2581 acccgggaaa tgtgggcttc taccgggcga acctagccgg ctggcaggat cgattcaagg
     2641 agaaagagaa gcagtgggcg gaatacccat tgagagggaa gaaattcctt ccctatcata
     2701 aattcttcga gtacctcgcc catgagttcg gtttcgagat cctcgggtat gtagagccga
     2761 aacccggcat tcctccctca gccggatggg tggaaaagat catagagctg accaaaaggg
     2821 tgaaacccga tgcgatcctg acgacctcct actatgggac ccgagaagtg acttttctgt
     2881 ctcagaagac cggggtgaaa tacatcgtgg tcccccatga tgtgggggca acaccggcct
     2941 gcaaagactg gttctctctg atggaccagg tcctcgcgga gctgaaataa ggagattcaa
     3001 tggaagcttt ccatttcctg atcgttccct tcgtgggatg cgtcgtcctg atcctgatcc
     3061 acgcctattt cggaatccac atccttgaac ggggagtcat tttcctggac ctctcgctcg
     3121 cccaatttat cgccgtaggc atcgccgtct cgtgttttac gggggatgtt cccgaacatc
     3181 ggttttttta tgccgccgcc ttcgcggtct tcggagcttc catcctttcc ttttccaagc
     3241 agatcgcccg gctggtcaac atcgaagctt tcatcggcgt gctgtacgta ttttcctttg
     3301 cggcgagtat tctattcctg gacaagagtg cccatggcac agaagaattc aagtccatcc
     3361 tgaacgggaa tattctttgg atgacccgca gggacctcct gatggcctcc ctactttatg
     3421 cgggaatcgg ggcctttcat ttgatcttct ggaagaaatt cctcgctctt tcctacggag
     3481 gagggaaggg gtttctctgg gagtttttct tcttcctgag cttcgccttc gtgttagtca
     3541 gctcggtgca gatcgccggg atcctgcagg tcttctcctt cctggtcatt cccgctctga
     3601 tcggaagact gtataaccgg aatccgattc aaatcctcct catcggatgg gggattggaa
     3661 tcgtggtctg cgtgatcgga gtctctctct cctacaagct ggatgtaccc acggcccccc
     3721 tcatcgtggc ctccctcagt ttgatctttt ttgtcctttt gggagtgaag gccagaaaat
     3781 cgggggccga ggtggaaaaa agataggaac gcacccctca aggaatccat gggaccaaag
     3841 agagatggaa aaaagcagga tccgattcct tacggtctcc ccgggggact ggctaccgca
     3901 ggttcttccc cgtggtggtc aaggagagct ttccgtgttt cactccccgg attccaatca
     3961 gccgatcgct gatttttttt atgtctcctc ccattccctt cactacgagg acctccaggc
     4021 agttgtgctc atccaggtgg atatgcaggg tggaaaggat ggttttataa tggtcgtgct
     4081 gcatcttggt taaaatgcgg gaaagctcgc gggtatgatg gctgtagacc aaggcgacgg
     4141 ttcccaccat ctcctgtttc ccttctctcc actcctcctt gaccaggctg tcccggatga
     4201 ggtcccggat ggcctcggac cggttctgat atcctttctg ggcgataagg cggtcaaaat
     4261 ctccgagtag actgtcggga atcgatactc cgaagcgaat aagtccggcc attcttcctc
     4321 cattttccag cgtattagag cgacgagatt tcggtgaggt ccttttcttt caacatcctc
     4381 tttttccagg aatgtagggc gggatagaaa taaggcaaga gccttaaaga ataataagag
     4441 tggaaaacag gaactcccag aatatcgccg aaagaaaaca aaagaatgag attaaggcga
     4501 gccagttttt cctgccggac cgtctgttga atctcaaagg ctgttgctcc aaagaaaaaa
     4561 tctttgagcc cctttttcag gtcctcaagc ctattgctca ctttcccctc cttcccgggg
     4621 ttctggaaga tggtcttggc cggcagatcg aatatcggca acttaatata ctctgaatct
     4681 tcttccccgt caaactcacg agattccttc gggcctaatt taaaatggac agagattttc
     4741 ccctggggta atatagggtt cgagagaggt atttttaaag ccagaccccc aaaaacgatg
     4801 ccttctcact gcctcaagag gattcgcctt gaaagagatt ttacgtcatg cccgggaagt
     4861 catccagatc gaagcagagg ccgttcaggg cctgctcgac cggttggatg acaatttcgc
     4921 ccgggccgtg cagcttctcc tggcctgcga aggacgggcg gtaatcaccg gcatcggaaa
     4981 gtctgggctg gtgggccgca aaatggtcgc aactctaacc agcacaggga ccccggccac
     5041 cttcctccac cccgtggaag gtttgcacgg ggatttggga atggtaacgg ccaaggatgt
     5101 ggtcgttgcc atctccaaca gcggagaaac ctccgaaatc atccatctcc ttcccagcct
     5161 gcagaagatc ggggccaaga ttatcggcct caccggttcc ctggattcca ccctggctcg
     5221 gagcagccat attgtcatcg atgtgggcgt ggagcgcgag gcttgtcccc tgggattggc
     5281 ccctacttcg agtaccaccg ccactctggt catcggagat gcgctggcgg tggccctgat
     5341 gcgacagaga aaattcggtg aaaaagactt cgctcttttc caccccgggg gaagcctggg
     5401 agagaaattg tccttcaaga taaaagacat tatgcgcccg gcggcggatt ttccctccct
     5461 ttcttccgac aagaccgtgg gggagttcat ctccgccatc aaccgctgcg gggctgattt
     5521 tgccctgatc ctccaggcca acggaggcct ggccggaatg gtctcggatc gcgaccttag
     5581 gcggggagag atcggctcgc ccgatttttt gaaccggccc attaagaaaa tcatggaccc
     5641 ccatccgccg acaacggaag aagaggtctc tgcagccgaa gccatgagaa ccatggtgga
     5701 aaagaacctg gtcgccctgc cggtcctgga caagaaaaaa aacttgaagg gcgtggtcac
     5761 cctccgggac cttctgaacc ggagagatgg gggggatgtt gtaaaatgaa tccctacccc
     5821 cccaaaggac tgatcgtggc gctcataact cccctgaacg aggagggcca ggtagagcgg
     5881 gtatctctgc accggttgat cgatcgggta gttccctact gcgatgccct gatgatcggg
     5941 gaggggctca tcggagaggg cctctccctg ccgaatccat ctcggctgga actccttcag
     6001 gtttcagccg aaatggtggc ggggaaaaag ccccttttct tatgccccac ggctgggacg
     6061 actggagaga cggtgaataa cattaccgtc ctcacccaag ctctgaagaa ttacccggat
     6121 caggactccc ttttctgggt ggacatccct ctctggtatc acagcaaccg gaatttgccc
     6181 cagttctacc aagaatggag ggaatacact ccctacccca tcctgctcca taaccaccct
     6241 ttgctcatcg gcaaacggaa ccgctccctc aagcggacca acattcgcac ggccgttctg
     6301 aagctattgg ctgaaaacgg gcagatcgtt ggcctcattc aatccggcga ccttaaaagg
     6361 actattcatt accagagagc tgtgcgggcc cgcagggact ttcgcttctt cgacggggat
     6421 gaaggaaatt tcctcaacag cccaagctct tcaggggtag tttcaggagg cgctaacctc
     6481 tttccttctg aatggagcga ggttgtcact gcttcgctta aaatgtccga ggacccggca
     6541 aagaatctgc ttcttctcaa acgaagccag aagctccggg aactcagcca ggtaatgcag
     6601 aagaatccgg cccagagcct caaagtagcc cttcatcgcc taggatggct ctcgggggcc
     6661 aagggtctcg acttcactca ggaaaactct ctggaagcag tagagggtct catcagcttc
     6721 ctccaggtgc atttttcttt acaaactccc tcctaaaatg ctattcctat acctgcaaga
     6781 ggatggagat acccgaaacc attaaagctt ttcccatttc cgaccgattc tattactgga
     6841 tcagtttgat ccccaatccg tttagaagcg caaaatagcg tgaacatctt tcttcgattc
     6901 aaaattattt gtacttatat cctattagct gctttttggg tgtcctgcgg ggcctgcggc
     6961 tacagacccg cctatttgaa ggaagggaag acagccgagg tgtcggagag gtggagggtg
     7021 aagaagatcg accccgccta tctttctgcc gatgagaaat ccgttctgga aaatttcggc
     7081 aaccctgatt atatccgctt ttaccggaag cgctcgctgg agcgggagcg agtctacgcc
     7141 tgggtctatg ccgatccggt tcgattcgtc actttcatag atggccacaa agtcgattac
     7201 gcggttctgg atgaagacct ctcatcgctg aatgaatatc agcgtaatat gcttttttgg
     7261 ggagggatta ccgctggaac cttggcagcc cttgggttgc tgtattactt tttgttcgcc
     7321 aagaaatagc caaaggagtg ccccatgatc aaactggtcc tcaagctgaa ggaaacccag
     7381 atcgaggagt ttgccctgga gaaagatgag atcaacatag gacgcgccaa ggagaatgat
     7441 atcgtcattg acaatatcgc tgtctcccgt aaacatgctc tcatccagcg gaaagtaggg
     7501 gagaactatg tcatccggga cctgaacagt tccaacggga ctttcctgga cggaattcag
     7561 atcgatgccg atgaccatac ccttaccgat ggagcggtga tcggaattgc caagttcgag
     7621 attatcgtaa aaggcctatc caagcaggtc cctcccccga cgcctaagcc cccggcctcg
     7681 gaagacgttg aaggaacgat gattttcgat gccgcccggc ggaaaccaac gcccgaagtt
     7741 caggcgccct ctgaacccaa gcccatccgc tggccagccc tttccgctac aaagggcccc
     7801 agcaaaggga aggaatacaa gatcacgaag gaagtcacct tggtgggcaa agacccccaa
     7861 aatgatatcc ctgccgaagg gtggtttgtt tcctcccccc atgccaagat cttccgccgg
     7921 ggagaccgtt tctacatctc ccacctgggt gggtttttat cgaccaccaa ggtgaatgga
     7981 atcgggatca gggaggatca catccttaaa aataaggacc aggtggatat cggaaattgt
     8041 acctttgtct tcacccaggc tcccgctgaa cataccgact gaccggtgag tttttctttg
     8101 gactggagct gaggtactct tgaagattcg ctatgcagcc gcttcagacc gtggccgggt
     8161 gaggaaaaac aacgaggatg ccttcttggc cgaccctggt ctgggattct ttgccgtagc
     8221 cgatggcatg ggggggcatg cggccggaga gatcgccagc cgcctggctc tggacaccct
     8281 tcggaaatcc atcgccgaat cgaagcagaa ccgagaaact gaattctcac ccaatcaaac
     8341 agctctcctc tcccccccat cgaccctgct ggtgaatgga atccgactcg ccaatcaggc
     8401 tgtgtttcaa tcctcccaag aaaaagaaga gtaccggggc atgggaacca cagtggtggc
     8461 cctttttctt ttcgattcat cctccgtggt cgcccatgtt ggcgacagcc gcctttatcg
     8521 gatccgagga gggagaatcg accaggtcac tgaggaccat tccttggtgt gggaacagta
     8581 ccgggaagga tccctttcga aagaggcact atccagctcg cccctaaaaa acatcgtcac
     8641 ccgggccctg ggtatgaacc tgacggttga tgtagacgtt caagaaatag acctgcagaa
     8701 gggggacctg ctagttctat gctcagatgg gttgtccgac ctggtgcagg atgaagaatt
     8761 gatgagcacg atccgcaggg cgtccggtga cctgaatcgg gcttgccacg acctcaccca
     8821 attggccaat cagcgcgggg gtaaggacaa tatcactacc ctcatcattc aaattgaata
     8881 gagaaggggc ccaggattgg agaaatggac ggttccagaa aaaacatctg ggcgtttgaa
     8941 tttccctaga ttctctgagt ccttgtgcct tttggagagt ttgggggcgt taccagcatg
     9001 atcctcaggt ccatgacatt cgtcccggtc ggcccgatgg tcaacaggtc tccgattctc
     9061 tgaaaaaaag aataagagtc gttctccttc agaaaagacc atgggtccaa tcccatcgtc
     9121 tttgcccgaa tcacggtctt tccgtcggca aaggctccgg cagcatcggt cggcccgtca
     9181 gttccatccg tgcctgcact catcatcagg atctcttcac agccagcgat ctccagcgct
     9241 ccagccaggg caaactcctg gtttctgcct cccttcccct ttcctttcag ggtcacggtc
     9301 gtctctcccc cggaaagaat gcaggccgga gggggaatcg ggttgccaga aagaaggacc
     9361 tctttggcga tggccgcatg caccttggcg atctcccgcg tttctccttc gacgagggaa
     9421 gaaaggagga gggtccggta tccaagagcc tgagctgctt ttctggctgc cgtcatggcc
     9481 aaaaggttgt ttcccacaat caggttgtag accttggcaa acgccgggtc tccctccttg
     9541 agcgtttctt cttttcttcc ctccatgcct gcccggatgt ggctggccac cgatggagga
     9601 atttttcccc acagctcgta tttgttcagg atccaggcgc aattttcgta agtcgtagag
     9661 tccgggaccg tggggccgga ggcaatggca tcgagcgggt ctccgatcac atcggaaagg
     9721 atcagggaca ccagcgtcgc cgggtaaacg atcttcgcca atcctccccc cttgattcgg
     9781 gagaggtgct ttcgcagggt gttgatttcc tgaatgctgg cgccgcagga aaggagctca
     9841 ttggtggtgg cctgcttctc caccagagtg accccttcca ccggcagagg caagagagcc
     9901 gagcctccgc ccgagacgag gaaaatgacc aaatcgtttc ccgtgaggtg gcttagcatc
     9961 tccaccatct ccttggcgcc ttccaggcct ttttcatcgg gcaggggatg gcccgcttcc
    10021 tggacccgga ttcgcttcaa cttctgggaa tggccgtact tcacattgat caagcctctg
    10081 gtaattcggg tgccaagtat cttctccaag ttcacggcca tggacgccga ggctttcccc
    10141 gttcccgcaa caaaaatttt ttcatatcgg gacagcgggt atttccgctc cccgatgcgc
    10201 aagacgtggt tcctaaggga gatatgggag gagaggatgg tctggggttg caccgcatga
    10261 atggcatgga ggaatatctt cttagcatcc ctccgcagcg ggtccaagct tcttttcacc
    10321 ggttcctcct tattcaggcg aaaaaccagt ttactctacg gtcacactct tagccaggtt
    10381 tcttggctgg tctacatccg tccccttgag aaccgccacg ttatacgcca ggagctgcag
    10441 ggggacggtc aaaaggatgg gagccaggaa agggtgggtc tccggcacca ggagggtgtc
    10501 ccgggccttg gaaaccatct cctggcagga aggattggtc agggcgatga cttttccgtc
    10561 ccgagcccgg acttcttcga tgttgctcag aatcttttcc tgggttcggc ccatgggggc
    10621 cagaaccacc accggcatgt cttcatcgat taaggcaatg gggccgtgct tcatctcccc
    10681 ggcgggatac ccctcggcat ggatgtaaga aatttccttc agcttcagcg ccccttcgag
    10741 ggcgatggga tagtttactc cccttcccag gtagaggaag tctcgggcat tcatgtactt
    10801 cttcgagagg gcttcgatct gggtgttcag ttccagagtt tgctggaggt aatgggggac
    10861 cttcagcaac tcttccagca gatccttaat tgtttgacgg ccgagggtct cccggttttc
    10921 ggccaggtac agggcgagca gaaaaagtcc caccagctgc gtggtgaagg ccttggtcga
    10981 ggcgaccccg atttccggcc cggcgtgggt gtagaagacg ccctccgatt cccgtgccag
    11041 gctgctgtcc acgacgttgc atatggacag ggtacgcatc cccttccttt tcccttcgcg
    11101 gaaggcagcc agggtgtcgg tagtctcccc cgactgggag atggccagga ggaggtcctg
    11161 ggaaccgacg atggggtccc ggtagcgaaa ttccgagccc aggtctactt ccgtgggaat
    11221 ccggctcagc ccctcgatca taaactttcc caccagggcg gcatgatatg aggtcccaca
    11281 ggccacaata cagacccgcc ggatatcctt gagcgctttg gaattcaatc ctgcgccttc
    11341 caggaagact ttgccttcct ccagggagac ccggccacgt actgtatcca cgatcgcccg
    11401 gggctgctcg aagatttcct tgagcataaa gtgcttatag ccgccctttt ccgcggtgac
    11461 cgggtcccac aggatttcct tggcgggccg ctgacggttc tcaccccaca gggtgctgat
    11521 ccggtaccgc ttctcctgga cgaccgccat ctccccatct tccagaaaga tcatctttcg
    11581 ggtgtaggga aggatggccg gaatatcgga ggcgacaaaa tgatctttat cgcccagtcc
    11641 caggatcaga gggctttcct tgcgcgcggc gatcatctta tccttctcgc cttcataaag
    11701 gacgacaatg gcataggacc cgtgcacttg gaggagggcc tggcgcaccg cctcttccag
    11761 cttctcgcct ttgaagatgt accgctgaat caggtggcag aggatctccg tgtccgtttc
    11821 ggatttgaag gtatgccctt ctcctgccag gatattcttg agagccaggt aattttcgat
    11881 gatgccattg tggaccaccg ccacgccccc tgccttgtga ggatgggcgt tggactccga
    11941 aggtttcccg tgagtggccc accgggtgtg gccgattccc agatttccgt taaatttctc
    12001 tccttcagtt ttccgctcca gctctttgag cttgcccatg gcgcggcgga ttaccacttt
    12061 gccctcatgg aaaagggcga ccccagcaga gtcgtatccc cgatattcca gtcgccgcag
    12121 cccttccaga aggacttcct tggtctccct gggcccgatg tacccgatga tcccgcacat
    12181 agttaacgac ctttctttcc cacagagagg ttacatatga atcccaagca ctaaatccaa
    12241 aaattctaaa tcaactttta aataccacaa ccaccgattt ttcgcatttc gcttttgaaa
    12301 aggggtgatt gtttggaatt ggcgtttcaa attgggtctc tgtgctctct gcgcctctgt
    12361 ggtgaaaatt atttatttcg aacgccgacg gtaatggacc tgtttccccc tggccaccgc
    12421 caaagtatct ggggggactt ccttggttat ggtcgatccc gctccgatga ccgagttgcg
    12481 gctgactttg atcggcgcca caagggaggt gttgctgccg atgaaaaccc cgtcctcaat
    12541 gaccgtggca tgcttctttt tgccgtcgta gttgcaggta atagtcccag cgcccacatt
    12601 gatcttctct cccagggtgg catccccgat gtaggagagg tggttggcct tggtgccttt
    12661 gccgatcacc gatttcttaa cctccacgaa gttcccgatc ttggacccct cccgaagcac
    12721 gttttgcggg cggaggtgcg cgaaggggcc tatctcaaca tgatcttcaa ggatgctttc
    12781 ggcaatcacc gaggaggctt tgatggtcac gtgattcccc gcctgggtat cgctaagctt
    12841 acagccgggt tccaccaagc atgcgatccc caaagaggtc tttcccagga gatagcaatt
    12901 gggatagatc accgtatccc ggccgatctt cacttcccgg tcgatgtagg tggtttccgg
    12961 tcctatgagg gtgacgcctt ccagcatgtg ccgttcggcg attaaacttc tcatgtaatg
    13021 attggccttg gccaactcca cccgggtgtt gatccccatt acttccagcg aattttccac
    13081 caggaaggga aggacttttt tcttctccga attggcccgg gccacaatat cggtaaggta
    13141 atactcctgt tgagcgttct gaggtgtcac ctgggacagg gcggaaaaaa ggaaatctgc
    13201 atccacgcag taaatgccgg tgttgatctc ccggaccttt tcttcccccg gtttcaggtc
    13261 cttatcttcc gtaattcgca ggagggaccc gtcgccattg cggaagaccc ggccgtagcc
    13321 cttggggttt tccagttcca cggtcatgac ggagagcgca gcctgatgtt cccggtgggg
    13381 tttgaggaat ttgcctactg tttctgaggt gagcaaaggg acgtcgccgg agaggattaa
    13441 aacgttacca tgaaatccct ggagggccgc ccgagtggcc aggaccgcgt gtcctgttcc
    13501 caattgttct ttctggatgg caaaagttac atcgtcggcc ttgaattttg cctgaaccag
    13561 atcagcctgg aaaccaacga ccgcaaccag gcgggagggt tttaggcttc gggccacatc
    13621 cagggggtaa gagagcatcg gaatgccggc cacggagtgg aggactttca ccaggtccga
    13681 cttcatccgg gtccccttgc ccgccgccag gatcacgacc gccagctcgc tcaagctttc
    13741 ctttccttcc taaggccttg atgggactga taatttcatt atagaagaac ccttccccca
    13801 gtcaaggttt ttctcttttt aaatccgatg gttaaagaga tgcactttag gtttacctat
    13861 ttttaatcgt caatttgaga atttttctta accttatctt tttcttcctg caaatattca
    13921 aacatacact ccctgtcctt ctttacggca tgtccgggat tcatgctttc cggcccggcc
    13981 ctttccctcg gaaattttcc catctcaaga acttggtctt ttgcagccag agggtcccaa
    14041 ggcatgtttt catcttgaca gtaaaaggaa aaaaattgag aatttcctta tggaattttc
    14101 atccctggtg gccacgctgc agaatcctca aatctatcct gaaaagccgg cagaaataga
    14161 actggtgcag acccatatat cggccatctt ttttgccggg gagcacgtgt acaagattaa
    14221 aaaaccagtc aacctcggct tccttgactt cacctccctt gaaaaaagga agtactactg
    14281 ccaacaggaa gtggacctca accggaggct ggcgaaggag gtttacctgg gggtcgtgga
    14341 ggtccgctcc catcaaggcc aaatatccat cggggaaagc ccgggaaaag tggtcgagta
    14401 cgccgtgaag atgaagcgcc ttccccaaga ctgcatgatg gaccagtggc tggctaaggg
    14461 agccgtaacc ccgaagatcg tggaaagaat cgccgcaaag atcgcccatt tccacgctct
    14521 cgctgccacc agcccggaga tctctaaatt cggaaatatt caaaccatcc ggggcaacct
    14581 ggaggaaaat ttctaccaga ctgagaaata cgtgggccgt gctctaacct cgaaccttta
    14641 tgaggagatc atggaggcca ctcgccggtt tatggatagc catacccccc ttttctacaa
    14701 gcggatcgcc gatggaatga ttcgcgactg tcatggagac ctccgtcttc aacacatctg
    14761 tatgggggaa gacattctca tcttcgactg catagagttc aaccatcgct tccgatattc
    14821 cgatgtggtc gcggacctcg cctttttgct catggacctg gattatcata aacaaccgct
    14881 tatctccgcc gacttggctt cccactacct gacgatctcc cgagactggc ccctctatct
    14941 gcttttggat ttctataaat cctaccgcgc ctacgtgcgg gccaaggtga cttccttccg
    15001 cttggacgac ccgaatatct cgacggaaac aaggtcttca accctgaagg aagcccggcg
    15061 atattacctc ctctcccatt actacgccaa aaggatgaac catctcctgc tagttgtcac
    15121 cggaggatgg atcgggactg ggaaatcgac catcgcccgg gccctcgccg aagctctggg
    15181 gtgggagtgg ctgcggactg atgttctgcg gaaagaattg gcccaggtct ctcccctgga
    15241 acaccggttt gaagaattcc atcagggggt ctattccccc gacttttccc aaaaaaccta
    15301 ccagaacctt ttcgagaggg cccggaacca cctggaatcg ggaaaatcgg taatcctcga
    15361 cgcctctttt aagaagcaag cggaccggca gatggctcga gacctggctc gaaataccca
    15421 ggcggatttc cttctcatcg aatgccaatg ttcggacgag gaaatcaaaa ggcggttggc
    15481 ccggcgggcc tgggaaaaga gcgaaccttc cgacgggagg tgggagcttt tggccgaaca
    15541 gaaaaaagat tacgatccgg tggagggatt cgaccccgac ctgattatat ttttgaatac
    15601 ggaacgtccc ctggaggaat gcctagcaca gattttccgg catctcttca tgagggctgg
    15661 acgggagctc tcccatgcgg tacattagta ctctcgaaga atcctgatct gcgctggtaa
    15721 aaatctcgcc gcgaggtggt ggacatgact cgatggagat tttttctttt cctgctcgcc
    15781 ggactctccc tcctctctgg ctgccccccg gaccccaaag cggtccaaaa agcatcgtac
    15841 cactttggat tgggcactag ctaccttcag caaggagacc ctacctccgc tctccgggag
    15901 ctgtatgaag cggaaaagtt gaacccccaa gacgcgcagg ttcagcacta cttgggcatg
    15961 gccttaagcg ccaaggggaa atacaccgat gcattggagc attatcgcaa agctctcgaa
    16021 ctgaacccca aatacaccga agtccacaac accatgggag cgacattcct ggagatggga
    16081 aagtgggagg atgccatccg ggaattcaag ttagtcctta atgacatcct gtacctcacc
    16141 ccctttttcg tctacaacaa cctcggctgg gcgtattaca agaagggcga cctgaacagc
    16201 gcaattgaaa attaccggaa ggccctcggc atgaagccgg atttcggcct ggcccactac
    16261 aacctgggtc tggcctaccg ggaccggaaa caacccaacc tggccattgg ttctcttcgc
    16321 caggcgacgg ttctggttcc cggacttttg gatgcccact tccagctggg caagctctat
    16381 ttcgagacgg gaaagaacga ggaggcggaa aaatcctttc aggaagttgt gcgcctcgcc
    16441 ccgcagagcg aaagcgccca gatggcccga caatacctgg acctgctcaa aaaatcccgc
    16501 aaataatcag acgattccca ttcccttcaa gagggaaagc ataactgccg cggccagaac
    16561 cgacccgatc tgcccgccgg tattggcccc catggcatgc atgagaagat agctcttcct
    16621 gttggccttc tgtccttcca cctgaaccac gcgcgcggac attggaaaag ccgaaattcc
    16681 agcggcgccg atcagggggt tcaccttccc gccggagaga actttcatga tcttggccag
    16741 gaagatcccc atggccgtgt ccagggagat ggccactaat cccaacccca gaacgataag
    16801 ggtctgaggc ttcaggaaga ccggcccgga catggtggcc ccgatggaaa tccccaagag
    16861 cagggtgacg atattggcaa tctcgttctg ggaagccttg acgagtcggt ctgtcacccc
    16921 actctccttt agcaggttgc ccagcatcaa ggtccccatg agcggggtcc ccatgggagc
    16981 gatcaggcag gttaccaggg tcactgcgat gggaaagagg atcttcacgg cgggggtgac
    17041 cgtcttcttt tggtacttca tgacgatctt gcgctcttcc tttgaggtga gcgcccgcat
    17101 gatcggcggc tggatgatgg gaaccatgga catgtagcag taggcggcca cggatacggc
    17161 tcccagcatg tggggggcat acttggaagt cacgtaaatg gccgtgggcc cgtcacacgc
    17221 cccaataatt cctatagcca ccgcctccag ctgggtgaac cccaagaaca aggccagcag
    17281 gagggtcagg aaaattccga attgcccggc tccacccaga aggaggatcc gcggattctc
    17341 caggagagga ccaaagtcgg tcatggcccc gattcccaca aaaatcaggc aggggaagat
    17401 ttcggtcaaa actcccgcat cgtaaaaatg ccgcagcaat cccccctctt ccatcaatgt
    17461 cgccgtagga atgttcacca ggatggcccc gaagccgatg ggcaccagaa gcaggggctc
    17521 catgtctttc ttgatgccca ggtaaaggag ggcacatccc accacaatca tcactccatg
    17581 gctccactga aagttgctga atcctgcgag taagctcgat aatccatcta taatcatggt
    17641 tagcctcgtc cttctttctt ccgctcgccc cacagcagta tcctattcat cccgtcaacg
    17701 accaggccga tgaggaatag aatcacgagc gtgcctccca ttccaagaac ggttagagtt
    17761 aaaccaaatt ccaggttctg catttttccc aacctctctt ccttccctca ttcaatccaa
    17821 gttgagaatc tcctacgttt tgccatcttt gtcaagcccc tcttgcaggt ttcttttaca
    17881 gctccacttc ttgtcccaaa tttttcttct gcgcccggcg gtacacggcc tgcgccacgg
    17941 aaacgtcctg cacggcattg cccacggatt tgaaataggt aatctcccgg gccgaccctc
    18001 ttcccgcttt tctcccggcc gccacttcac ccagttcggc caagatatcg gtctcttgaa
    18061 tcagacctct cgtcaagggg atgatgaggt cgccggcctc ctcaagacag gcctctaggg
    18121 aatccacaaa aatccgcgaa cggcatacgg ttatctcatc cacctcctgc atctccggtt
    18181 tgaaagcccc aatggcgttg atgtgcattc cttccctcaa ctgcctgccg tcgaagaccg
    18241 gctctctgga ggtggtggcg gtcacaacga tatccgtccc ttccagcatc tgggtcgggg
    18301 tcccagcccg gagcagttcg accccgcggc aggaagcgtc tgcccccagc gcggcgatca
    18361 gcccatcagt cctctccgga aggagatcga aaaccttgat gcgttcgatt tttcgggccg
    18421 ccagaactcc cttcacctga aagaacgact gtcctccggc tccgaatacc gctagagacc
    18481 tggaatccgc gcgggaaagc acctgggtgg ccgcgcccgt ggccgcgccc gtgcggaacc
    18541 gggtaaggtg agtcccttcc aggacggcaa ggggagaacc ggtggcggca tcgaagagga
    18601 agacaatcgc ctggacggcg ggcaaatgag cccgcgggtt ccagggcaga accgtaacca
    18661 tcttggcgcc cagagcccgg gattcttcca aataggcggg catgaccagc gcagcttccc
    18721 ccggctcctt caggttcagc gaggtacgga ccggggattg ggcctttttg agggaaagct
    18781 gaacgaaagc atccttgacc acttcgacgg cttcggtcat ggaaagggcc tggtcaatat
    18841 cccgggccgt aaagattttg attctcatat cgctcctttg gccttcaact ccttgatctc
    18901 cgcggatgaa taccccagaa ggtccaccag gaccacttca ttcgccgatc ccatgggaag
    18961 tccgggaaac cgaacctggc caggagtctt gaggaaacgg accggcgcct cgggaaagga
    19021 aagcgtcttc cccgaggccg ggtcatacac cgatgccagg cttcctctct gttgtacatg
    19081 gggatcgttg gcaatgtcct ccatgttatt gaccggcccg atcgtgaccc ccgctttctc
    19141 gcactcttcc aggaccccct tcagggttct ggcccggatc cagtccccga tgatccggtt
    19201 caggacggcc cggctttcat tctggagcct ttcctgatta ttgcggaatc cggcggtcgt
    19261 cttcagttct gaatggccca tcaagtccat catccgctcg aagggcgcct gagcactggc
    19321 cgagagggct acccacttcc cttccttggt ctggaagctg ttccggggag aggtgaagtt
    19381 ggactcgttt cccatgggtt gaggattatc cccggtcagg gagtaaatca agaactgccc
    19441 tcccatgtat cccaggaggg gttcatacag agaaacttcg aattcctttc ctcctcgctc
    19501 tccccgtttc tggtctcgga gggcaaccat ggctcccagg gcaagatggg tcccggcaat
    19561 catgtcggcc agggcgatgg gggggttggt gggaggagct ccggggtggg tgttcatgta
    19621 agtgtatccg ctcatgccct cggccagggt cccgaatccg ggcttgttcc ggtagggccc
    19681 gctttggcca tagcctgaaa tccgcccgat gatcaagcct ttgttaatct tccacagctg
    19741 ctcggcggag aagcccagcc ggtccagggt tcccgggcgc atgttttcga aaagaacgtc
    19801 cgatttttct accagctgga cgaggatctt tttggcttcg ggatgcttca ggttcagggt
    19861 gatgggcagc ttgtttctgg aagccacgag ccagaagggc tccatatcgt tattcaccct
    19921 ggcccagtaa cggatggcgt cgggaacatc cggaggctcg atcttgatca cttcagcccc
    19981 aaagtctccc aggagggttg ctccaaaggg agcggcgacc accgccccca aatcgatcac
    20041 ccgcacggat ttcaggggca ggtctgattc ttcagccttt tttctttgtt cgcggaggta
    20101 attgtgaatc ttagaaaagt ccatgggaaa tctcctgaag ggaagttgct gggcgtctat
    20161 gccttcgcag ggaagaaatg aagcccgaat aaattttata acagaaaagg ttcgaaaagg
    20221 gatcaaaaaa agccggccgt gcctgaaata agagctgcat gccagggtag aagggaatca
    20281 aggcctaaag gataaaggaa gccgccaggc agcggaggag gtttagggtc aagggatgga
    20341 atggggacgc cagagatttg gtgaaaatgc tgctcaaggc ccaagtaagg tacaccccag
    20401 agcactgatc tctccgatcg cactctgttc ctttcccaat attcttatcg gtgaaagcat
    20461 cagaatgtgg ggatgaagat ttgagcgggg gctggttgat cggagggtaa tgggcgtctt
    20521 gattccgcta tccgcattcc aaatttcgcc ctttttatgg tggtcccaac gggaatcgaa
    20581 cccgtgtttt cggcgtgaga ggccgacgtc ctaggccgct agacgatggg accctcaaag
    20641 aatagaaaag aaacctaggc tgggagccta ggatttgcac ccaggtttgc aggtcctgag
    20701 cttgcgatcc tgccactgga tgatccctta ttttccccca gaatccactc ccattatatt
    20761 tttaattcct gaaccacgtc aaggcgataa tggccatgcc tcgggtctcc gagctccccc
    20821 tgaaccattg gagctacagg gcgatgctac gcatcattta gtgcaggccg aattgccaaa
    20881 tgcggaattg ggaattcctg agcaaggccg cgaatatgat ataatgcccc cgcatcttcg
    20941 gatgggaggt ttttatggac tatcggtcta ttctctacca aaaaaggggc gggaaggcca
    21001 tcataaccct taaccgtccc cgggcgctaa atgctctgac ccaggagaca atcgaggagc
    21061 tgctcaatgc gatccaggag gcctctctag accccgaggt aagagtcctg attctgaccg
    21121 gggcagggcg agccttttgc ttcggcgccg atatctccga gttcagccga caaatcgcaa
    21181 cgggtacccc tgattccgga atcctccttc tccgaaaagc ccaggaagtt atacgccttc
    21241 tcacctccat ccccaaacca accattgctg ccttgaatgg atttgctacc ggcctgggac
    21301 tcgacctggc tttagcctgc gatctgaggg tggctgccca acgggccaaa ttggctgagg
    21361 ctttcatcac catggggctg atcccggatg ggggagggac tttcacttta cccaggttgg
    21421 tgggactcgc caaagcggca gagatgatct tcattggaga acctctcaat gcctcagagg
    21481 cggaacgaat cgggctggtc aaccgcgtgg ttcccaccga agaactcgct aaagcaacgg
    21541 aaaacttagc cgataaactg gctaagagcc cctgtttggc tctcggcagg gccaaacagg
    21601 ctttatggaa aaatctggcc ggtgatttgg cctcggctct gaaggtcgaa gcccaaattc
    21661 agctgacctg cctagccagc gatgatcacc gggaggccgt ccaggcattc ttggagaagc
    21721 gggaaccggt ctttcgggga acctagaaaa atgtttcacc ggccctggag gagaaatggg
    21781 tctcggtcat tgtctttggg tattgacaga gtaaaactgc ttggttatca tagggtgcgc
    21841 tttaggaccc taatgaggag aggagatttt ttgaaaatag ctttctgggc cctgttcgtg
    21901 agcggatttc tcctcctggc cgcatgcagc ggaatccaaa acaaggttac tcaaagtttc
    21961 ctctctccat ccccgggtca gttggctcag gaaatcactc tcattccctc aaaccgcgca
    22021 aatatgggcg tccgtgtatc tccgggcacc taccctttac ccgaaatcca aaccgatgac
    22081 ttgaccccct caactcaaga ggcggagtta aacttttgct ttgccgggga gatgccgctg
    22141 gaggggagtg aacctccatc gatcgatact ccggggccag ccttcgttct tctgaggaaa
    22201 gggtgggaaa caaccgattc ggaaatgcaa gggaaagggt ctattctcct tgattcatct
    22261 ccagggaatg aaaataaggg agaagggaat ccctcgtttg ccgtaaaccc gctttccagt
    22321 gagtctctcc ccttgaagaa tacgaacgcc tttgaagagt cttttgaacg attgaaggag
    22381 attttgaccc aatatgaaat ggagtccatc tccccgagtt cctcatcccc agtaattggc
    22441 ttaaatggca agctccccga aggagaaacg aggacgaccg gaatttcggc cgcttttccg
    22501 acactgctca acgaccgggt gaaaacctat atcgatttat tccaaaacaa agccgatcat
    22561 ttcttcacca gatcactggc ccgctcccag gcctatgaag ggatcataag gcccattctc
    22621 cgagagaaga acctccccga agaactcttt tacctggctc tgatcgaaag cgggtacaat
    22681 ccctacgccc agtcgcgggc caaggccaca ggaatctggc agttcatgaa agggaccgcc
    22741 aaacgctttg gcctcagagt ggatcaatgg gttgatgaac ggagagaccc ggaaaaatcc
    22801 acccttgccg ccgcagatta cctgaagagt ctttatgaaa tgtttaactg ctggtatctg
    22861 gctgcggccg gctacaatgc cggagaagcg aaaattcttc aggccatgaa gcgggccaaa
    22921 agccaggact tttgggccat ttcccaacaa cggtacttga aaagggaaac caaggaatat
    22981 gttcccagac tgctggccgc catgatcatc gcccaagacc ccgctaaata cggtttctcc
    23041 aacattgatt accatccgcc cctggtgtac gaaaaggttg tcgtcccctc gggagtccgt
    23101 ttggaccgaa tcgcccgggc tgcggaaacg acggtcgagg aggtccgagc cctgaatccc
    23161 gctctccggc gggataaaat ccccccgcac gacccctctt ttgagatcaa actgcccccg
    23221 ggcaagaagg aagcctttga gaaaaacttc tcccaggtgt tcaaactgga ccctcgtaca
    23281 gccaggaaac acctggtccg ccagggggag acgctgggtc aaattgccaa gaagtacaag
    23341 gtcgaccttc gggagctatg cgaaggcaat gaaatcaacc cgaagaccct gataaagcca
    23401 gggatgactc tccttcttcc cccctagggc tcctagcgaa catgatcctc gtagaagaag
    23461 ccctccatca tattctggga aagatttccc gcttggggtc cgagcgtatt cctatccttc
    23521 aggccctggg ccgggtcctg gcagaaaaca tcttcgcctc ccggaatata cccgcctggg
    23581 acaactcggc catggatggt tatgcagtcc gggcggaaga cattctcagc acatcgaaag
    23641 aaaagccggt ggttctaaga gtcctcgggg acctgcccgc cggcaaagtc tttaaaggcc
    23701 ggggtggctc cggagaggcc gtgcggataa tgaccggggc tccccttcct ccagggtttg
    23761 acacggtcgt cccagtagag gatacagaga aggacgggga acgggtaaga atcctggaaa
    23821 cccccggaaa gggaaaaaac atccgccttt ccggcgagga tgtgaaatcc ggggaacgga
    23881 tcctggaaga aggaaccctt ctccgccctg cccacatcgg catgctggct tcgatccagc
    23941 gctcaatggt ctccgtatac caacaacccc gggttgccgt tttatccacc ggagacgaac
    24001 tcctggaaat tgacgaaccc tgggaagacg ggaagatcgt caatagcaac agttattccc
    24061 ttgccgcctt ggtcgctgaa tccgggggca ttcccatcca actgggcatc gccaaggacc
    24121 gcagggaaga gctcaccgcc aagattcggc agggtctggt ggcagacctt ctgctgacct
    24181 cgggaggcgt ctcggtaggg gattacgacc tagttaaaga aatgctcaat gagctgggcc
    24241 agatgaactt ctggaaagtg gccatgagac ccggtcagcc cctggccttt ggaatgatct
    24301 cagaaaaacc gctcttcggg cttccgggta atccggtttc ctccatggtc tccttcgagc
    24361 agttcgtgag gccgtctatt cgcaaaatgt ccggatacaa ggatttattt cgtccggtgc
    24421 tccaggccat cctaagggaa gatatttcta aaaaggccgg cttggcccac ttcatccggt
    24481 gccgcttgat tttagaaaag gggaaggtct atgcaacgac cacaggggag caggggtccg
    24541 gaatcctctc ttccatggta aaggctcagg gcctgatcgt tcttcctcgg gaagagaccc
    24601 tggcccgggt tggagaagcg gtcaaagtca tccttcttga tccttccttt tttcatctct
    24661 ccggccccga ttttctttcc tgactttcgc aacctatgaa tagggcctaa cattcaattc
    24721 cttgttgccg ctgtgggaaa aaaaataaaa aaccgttgcc atattccccg ttctggggta
    24781 tagatattta ttactccttg ctctatcgct ctgcgcgaag ggctatccaa acccatgggg
    24841 aggaaccacc atttgcgacg ctatgagact attttcatca cccaccccga attgaccgag
    24901 gaagagcttt ccgaactcaa ggagaaaatc ctttccatcc tgacccagtt caaaggcgat
    24961 ttaatcaagc tcgatgactg ggggattaaa aagctgacct tcgaagtccg caggaactcg
    25021 cggggccgct acttcttgat agattatgcg gccggcaaag accttatccg ggagctggaa
    25081 aggaacttgc gtctgaatga tcgggtcctg cgttttcaga cggtgaggac cagcgatgag
    25141 ctcactcctg aggctatcaa gacccttaag gaaagcgcgg ggcctgaaaa agccaaaggc
    25201 agcgaacgga tcgagcctcc aacgggcatc aaggggggag atgaggccca ggtagaagga
    25261 ggtgaaaagg aatgaaacct cctgccacga ggaagaaacg tccttttcag cggcgtaagg
    25321 tctgcgtatt ctgcgccgac agcggtataa agattgatta caaagacacc aagatgttga
    25381 cccatttcat caccgaacgg ggaaagattc ttccccgaag aatatccgga aactgcgcca
    25441 aacaccagcg ggagctgacc acagcaataa agagagctcg taatatcgct ctcctgccgt
    25501 ttacggtgat cagcacctag cggggcatag gcccggtctt cggcccgtct cctcttggcg
    25561 ggccgtaagt ctcctttgat cgattagacc ttcagggcaa ggctggggga aacctcttta
    25621 cctaccatcg gcatcgataa gaatcgctgg aggttcacat gaaagtgatc ctgattgaaa
    25681 acgtcccttc tctggggaag ccaggggaca ccgtccaagt cgcggaaggg tacgggagaa
    25741 atttcctcat ccccaagaaa atggctctgg aggcaacccc tgccaatctt caccgcctgg
    25801 aacagcagcg ggaggtcttc ctccagaaag gggccaagga aaagcagtcg gcctccgatt
    25861 tggcggcgaa gcttgatgag ctttcctgta ccctggcccg ccccgcaggg gagaatgaaa
    25921 aactcttcgg ctcggtaaca agcatggacc tgcagagatt tctccaggag aagggcatct
    25981 ccctggatcg gcggaagatt ctcttgcctc agccaatcaa aaccttggga agttattccg
    26041 ttcccttgaa gctgcatccg gaaataaccg cccaattgaa ggtgaacgta gtggccgctt
    26101 cccctgaaga gaaagagaag ccttaggcga cctcccgaac gattaggttt taaaatggac
    26161 ttgctggccc agcgccttcc cccgcagaac ctggaggcgg aaatctccgt cttggggggg
    26221 gttcttctag acaatgaagc tctcaaccgc gtgttagaag tcatcaagga aggggatttt
    26281 tatcgcgaat cccaccgtaa gatcttctcg gccattcttg acctttacga acggagcgaa
    26341 cccgtcgacc tgattaccct ggccgaagcg ctcaggaagc gcgaagtcct ggaggaggtg
    26401 ggcgggatcg agtacctcaa ttccctggcc aacagcatcc ccacagcggc taatattacc
    26461 tattacgcca aaatcgtcaa agagaaggcg accctccgca agttggtcaa ccgggccaca
    26521 gagatcgtaa gccaggtcta tggccattcc ggagacgtag atgacttttt ggaccaggcc
    26581 gagcgctcca tcttcgaaat ttcggaagac cgcgtccgtc cttctttcta ccccattaaa
    26641 gacatcatca aatccagctt taagaccatt gagcggctct acgagaagcg ccagctcatc
    26701 accggagttc ctacgggatt cgccaagctg gatgaactga cctccggctt ccagccttcc
    26761 gacttgatca tcgtggccgg gcgtccctcc atgggaaaga ccgcttttgc cctcaacatt
    26821 accgcccatg cagccatcga agcggcggtc ccctccgcca tcttctccct ggaaatggcc
    26881 aaagaacagc tggcccttcg gatgctctgt tcagacgcca aggtggatgc ccatcgcctg
    26941 cggggtggct ttctcagcga atccgattgg cccaagctca cccgagcggc cgggtccctc
    27001 tccgaggcac ctatctttat cgatgacacc cctggactta ccgccctgga aatgcgcgcc
    27061 aaaacccgtc gtttgaaggc ggagcacaac gtagggctgg tggtggtgga ctacctgcag
    27121 ctcatgcgtg gacgggccga ttccgatacg cgggaacagg aaatatccga catctcccgc
    27181 tcgctaaagg ccctggccaa agaactgaat ctcccggtca tcgctctttc ccagctgaac
    27241 cggcgggtgg aagagcgcgg tgataggcgt ccccagttgg ccgatctgcg cgagtcggga
    27301 gccatcgagc aggatgccga tgtcatcatt tttctctacc gcgatgaggt ttacaataag
    27361 tcggaggata atcccaacaa gggaaaagct gaaatcataa tcggaaagca aaggaatgga
    27421 ccaaccgata aattcgaact gactttcctg gacaaattca cctgctttga ggacattacc
    27481 tatcgccagg aggcccgtta actatttaat tatatgaatt tatttgctaa tcagaattat
    27541 tcacacctgt taataaactt gtgaatagct ttcttcggga gatgtttctt cgagggtaag
    27601 aggatgaaag aactttggaa tagagtctta gcggatgtgg gggaaaagat tagccagaag
    27661 gcctacgata tctggcttaa accccttagt ctgattagcg taaaggagac tgaatttttc
    27721 cttgaggtcc ccaataaatt tttcaaggat tggatctctg agaattacca gaatttgatc
    27781 aaggaatcgc tttctcacct tactggacag cagtatacga tccaattcat catcaaagaa
    27841 aacgcagagg aaaagccgca aaaccccaag gatgaagaga gaatatccat tccgcggccg
    27901 gcgagacagg cagtaaggga agatggcctg aaccccaatt acacctttga tgatttcata
    27961 gtcggctcaa gcaaccagtt tgcccatgcg gctgccctag ccgttgccaa ccttcccgcg
    28021 aaaaattata atcctctctt tatctacggc ggcgtagggc tgggaaagac tcatcttcta
    28081 aacgccatcg gcaatcagat cgcggaaacc gatccgtcgg ccaatatctg ctacacgact
    28141 tctgaaaaat ttaccaatga actgataaac tgtctgcgtt atgaaaaaat gaccgacttt
    28201 cggaacaaat accgaaataa agatgctctc ctactggacg acattcaatt tctgggcgga
    28261 aaggaaagaa cccaggagga attcttccat accttcaact cgctatacga atcccatcgt
    28321 cagatcgtga ttaccagcga taaactaccc aaggatatcg gcggattgga agaacggctc
    28381 aggtctcgca tcagttgggg attgattgcc gacattcaac ctccggatat cgaaacaaag
    28441 gtggccatcc tatgcaagaa agcagaatta tttaatattt ccctctcgag cgaggtaggt
    28501 ctgtttatgg cctccaatct gggaaacaac attcgcgaac tggaaggcgc tttgacccgt
    28561 cttcgagcct attcatctct gacagggagt gaaatttcca tttccatggc caaagagaca
    28621 ctcaaggata ttctcagcga ccgtcagaag atgatcacca tcgatagcat ccaaaaggca
    28681 gcggcggcca ctttcaatat ttccgtatca gaccttaaat cttcaaaaag acttaagatt
    28741 tactcaattc cccggcagat ttccatgtat ttgtgccgaa ctatgaccaa gtgttcttat
    28801 ccagaaatcg gtgaaaaatt tggcggaaaa gaccactcta cagtcattca tgctgttcga
    28861 caggtagaaa aaaggctgaa tgaggaccgg gacctcagta acctcattga aaaactgaaa
    28921 aaccaactgc acaagtgagg atttccctgt ggactaaatg ggaatccttt aggactaaat
    28981 ctattcagtt cataaactct cttttgtcca ctttccttac caatcttttt cactctgaaa
    29041 atgaattaaa ccaattgaaa tgtatccaaa atttttatat aatcatccta tgaacaggta
    29101 ttactactac ggctatctaa atatattata tttagtatta gtataaggga gagtgagtat
    29161 ggaattttta atcgagaagg gagagtttac caagggcctc tttcgttccc agagtgtggt
    29221 cgaaaagaag ggaaccatgc ccattcttct gaatgtcctc atcgagacga aggacaacgg
    29281 tatattcatc accgcaacgg acttggaaat tggaattaaa gggcaatatc agggagatgt
    29341 acaaaaaaca ggaaatatca ccgtttcggc caaaaagctg tatgaagtgg tgaaggaact
    29401 tccggaaaag aatgtttcct tccggatgaa agaaaatcaa tgggtggaga tcaaaagcgg
    29461 aaaatctgtt tttaacatta tgggagtttc ggcggaaact tttccatcct ttcccgcgta
    29521 tgaagaagaa gaatttatgg ccatggaggc atccgttcta agggagatga tagataagac
    29581 catctttgcc gtttccacgg acgagtctag atacaacctg accggtgttt tccttgccaa
    29641 acataaggaa gaggaagatc aaaaaatgcg catggtggcc accgacgggt accgcctatc
    29701 cctcattgac cgaaatatga agacggaaat tccagggttg gagaaggggg tcctcctgcc
    29761 caagaaggga ctggctgaac tgagtcgcct tttggaagaa ggtggagacc aggtctggat
    29821 caaactgaaa aacaacaacc taatcatcaa aagggaaaat gtggttttga tcatgcgcct
    29881 gctggatgcg gagtttcccg attacaacca agtgattccg aatcagacta agcgtcacat
    29941 ccgcataaaa cgaagcccgt tcatagattc cctgaagagg gtttccattc tgtccagcga
    30001 aaagaccaag ggggtcaaat tccatttcgg cgaaaacctg ctggaactct cctcctacaa
    30061 ccctgacttt ggcgaagcca aggaagagat ctccgtcgag tacaggggag aggaattgac
    30121 cgtagggttc aattaccgat ttgtcctcga agtgctgaac atactgagca gcgaagaaat
    30181 tatcatggag ttcgaggatg gggtgagccc ggccgtcatt cgtccggcca acgacgaaaa
    30241 acacacttgc gtggtgatgc ccatgcgaat ttaacgaaag gtgagacgat tggccaagaa
    30301 gaacgataag gaaatcattc agagtggagg acccaaagag tataccgcag aaaatatcaa
    30361 ggttctgcag gggctggaag cggttcgcaa gcggccggcc atgtatatcg gcagcacggg
    30421 catcgccggg ctccatcacc tggtttatga agtggtggac aactccatcg atgaggccct
    30481 gggcggttac tgtaataaga tcgatgtggt aattcacgaa gacaagagcg tcacggtaga
    30541 ggacaacggc cggggaatcc cggtcgacct ccacaaggag cagggcaagt cggcggcgga
    30601 agtggtgatg accaccctgc attccggcgg caaattcgac agcgacagct atcagtattc
    30661 cggcggactc cacggcgtag gcgtctccgt ggtgaacgcc ctttctgaat tcctggagat
    30721 ggagatctac cgggacggaa aagtttattc ccagaggtac gagagaggcg ttccggttac
    30781 tccttttgaa actatcggca acaccaaacg gcgggggacg aagatcaaat ttcgccccga
    30841 cacggaggtc ttcgagaccc tggatttcag ctacgacacc ctttctcaga ggctccggga
    30901 gctgagcttc ctgaacagcg gtctgaaaat caccgttgtc gacgaacgga cggataaaaa
    30961 acaggagttt caatacaagg gcggcatcgt ttccttcgtc gaatacctca acaagaacaa
    31021 gactacgatt caccctaaac ccatccgtct ggagggggaa aaagagggcg tctacatcga
    31081 catcgccctc cagtacaacg acgggtacac agagaacctc ttctccttcg ccaacaacat
    31141 caacaccgtc gacggcggaa cgcacctgat cggcttcaaa tccgcgctga cccgtaccct
    31201 caacaattac ggcgccaacc tcagtcagtt taaaaacctt aaggccagcc tctccgggga
    31261 cgatgcccgc gaaggcctga ccggggtcat cagcgtgaaa ctatccaatc cccagtttga
    31321 gggccagacc aaggccaagt tgggaaacag cgagatcaaa ggtcttgtcg aggcgttggt
    31381 caacgagaaa ctggccactt attttgaaga aagtccgtcg gtggccaagc ggatcctgga
    31441 gaaagtcatt gatgccgccc gggcccggga agcggcccgc aaggcgcggg acctgacccg
    31501 gaggaagggt ctcctggaga gcgattctct cccggggaaa ctggccgatt gccaggagaa
    31561 ggacccgagg gagagcgaac tcttcattgt tgagggggac tccgccggag gctccgccaa
    31621 gcagggaaga gaccggaaaa tccaggccat cctgcccctg cgcggcaaga tcctcaacgt
    31681 ggaaaaggcg cgcttcgacc aggtcctttc caaccaggag atcaagaccc tgatcaccgc
    31741 tctgggaacc ggcgtggggg aagaagaata caacctggac aaacttcgtt accatcgggt
    31801 catcatcatg accgacgccg acgtagacgg gtcccacatc cgaaccctgc ttctcacgct
    31861 cttctaccgc cagatgtttc cgcttatcga gcgcggccat ctgtacatcg cccaaccgcc
    31921 gctctaccgg gtcaaaaaag ggaaagagga gcccaaatac ctacggagcg aggaagacct
    31981 ggaagattac cttctcgaga aagggatcga gaacatgcgc ctccttcttc cggaggataa
    32041 agggcagctc agcggcaaag gcctcctgga gatggtcaaa aaggccatcc gcctggacaa
    32101 gatcctccac cgcctggcca aacgaaagat ggacccggac attctcctgg ccttcgccca
    32161 gcaggaagct ttcagcaaga acaccctgaa atccgaaaaa gaaataaacc gggtcatcga
    32221 gcaggtgaaa aaggccctga aaaaggagaa aaagaacaag ggggagatgg aattccagat
    32281 ggaaaaagac ccggagcacg gttcctacaa cctgcgctgc gttacctcca ccaacgggtc
    32341 tcggctggag acgagcctgg acctggaatt ggtcagctcg ggccaattcg aggagctgcg
    32401 caaactctcc aaacagctca gttccctcgg tccggctccc tttgttctga aaaccggcga
    32461 gtcctcccag gaggtggcgg gtctgtctcc tctgatgcag gaggtcttct ccggggggaa
    32521 gaaagggctt gagatccagc gctacaaagg cctgggagag atgaacccgg agcagctctg
    32581 ggaaacaacc atgaacccgg aaacccgcac cctccttcag gttcgcctgg aagacgcggt
    32641 caaagccgat gagatcttca ccgttctcat gggggaccag gtcgaacccc gccgggactt
    32701 cattcagcgc aatgccctga gcgtgacgaa cctagacatc taaggaaaat ccaaattgca
    32761 gatgtcaaaa tgcaaaatgg cgacattttg ggatgtaatg gagcattttg atttttgaag
    32821 tttgcagttt gaatgtagga gtcgatatgg cttcccaccc cggattgaac cagctgccgg
    32881 tcaagctcga agaagagatg cagaaatcct acatggacta tgccatgtcg gtcatcatcg
    32941 gccgggccct gcccgatgcg cgggacggac tgaaacccgt ccaccggcgg gtcctgtacg
    33001 ccatgcacga gctctccaac gactggaaca agcccttcaa aaaatcagcc cggattgtgg
    33061 gagacgtgat cgggaaattc cacccccatg gggatgtggc agtgtacgac accttggttc
    33121 gcatggccca ggacttttcc ctccgctatc cgctggtgga cggccagggg aacttcgggt
    33181 ccgtcgacgg cgaccctccc gcggccatgc ggtataccga agtgcgcatg gcccgcctgg
    33241 cccacgaact cctgtcggac atcgacaagc agacggtaga ctttaccccg aattatgacg
    33301 gctccctgaa cgaacccgcc attcttccgg ccaaaatccc caacctgtta atcaatgggt
    33361 cttcggggat cgccgtgggc atggccacca acattccccc gcacaacctg aaagaagtga
    33421 tcgaagccac catcgcagtc attcagaacc cggacatctc cgtggaggag ttgatccagc
    33481 tcgttcccgg gccggatttc ccaacgggag gattcattct gggaaggggc ggggtccgcg
    33541 aggcctacct gacggggagg ggaatggtcc agatgagggc ccgcgccctg atcgagaggc
    33601 agaagcggac cgagcgcgag tccatcgtga tcaccgagat tccttaccag gtcaacaagg
    33661 ccagactgct ggagcgcatc gccgagctgg tccaggacaa gcgagtcgag ggaatcgccg
    33721 acctgcggga cgagtcggac cgggaaggca tgcgggtggt catcgagctc aaacgcgacg
    33781 ccgtggccca ggtgattctg aaccaactct tcaaacacac ccagatgcag gccaccttcg
    33841 gcgtcaccct cctggccatc gtgaggggac agcctcagct tctcaacctg aaggagttgg
    33901 tggagcagtt catcctccac cgcaaggagg tggtcacccg ccgcagccaa ttcgagctgg
    33961 gcaaggccga agcgcaggcc cacatcctgg aaggcctgaa aatcgccctg gacaacctcg
    34021 accgggtcat cgccctcatc cgcgcttcca agacacccaa ggatgccagg gaagccctca
    34081 tggccaagtt ctctctttcc gaaatccagg ctcaggccat tttggacatg cgcctgcagc
    34141 gcctgaccag cctggagcgg gagaagatca acgaagagta cgccgaggtg atcaagctga
    34201 tcgcccggct gaaggaaatc ctggggaacg agcgcattct cctcaatgtg atcgtggaag
    34261 agctccagga ggtccgggag cgctacgggg acgaacgacg gaccgagatc atcgaagaag
    34321 tgcaggagat caagctggaa gacctgatcg tggaagagga tatggtggtc accatcagcc
    34381 acaccggcta catcaagcgc aacgccatca gcctgtaccg gagccagaga agaggagggc
    34441 ggggcaagat ggggatgacc accaaagaag aagatttcgt cgagcatctc ttcatcgcct
    34501 ccaccctcag ctacattctc tttttcaccg atgcgggaaa ggtgtactgg ctcaaggtcc
    34561 atgaagtccc ccaggccggc cgaatgaccc gcggcaaagc catcgtcaac ctgctaaacc
    34621 tttccgggaa cgagaagatc accgccatcc ttccggtgcg ggcctttgag gaggggaagt
    34681 atgtcctgat ggccaccaag caggggatca tcaagaagac cgacctcatg tcctacagcc
    34741 accctcgggt cgggggcatc atcgcccttt ccctggatga gaaagaccag ctcatctccg
    34801 cctgcctgac cgacgggacc aaggaagtcc tactcagttc ccgcgaggga aaggccatcc
    34861 attttgtgga aggggacgtg cgagacatgg ggcgcgccgc ccgaggggta aaaggaatca
    34921 ccctgggcaa ggacgatttc ctggtttcca tggacatcct gtcccccggg gcggtggggt
    34981 cgtccatctt atcggccacc gaaaacgggt tcggaaagcg ctcccccatc gaggattacc
    35041 gtctgcaaag ccggggtggg aaagggatca tcacggtcaa gaccacggcg cgaaacggga
    35101 agctggtggg cacccagcag atcaccgacc aggacgaagt gatgctcatc accgaccggg
    35161 gcaagatcat ccgcctgcgt tgcggtgaga tccgcttgag cagccgcaac acccaagggg
    35221 tcaagctcat cgagctggag tcaggggaga gggtgatggc cgtggcccga ctggcggagc
    35281 gggaagagga aggaaacgga caggaagggg aagaggtccc ggaagagccc tgccccgagc
    35341 ctgagtcctg aaccatgaac cggggcagga gaagaattgc cgtcatcgga gctgggagct
    35401 ggggcacaac cctggccaat ctcctggcgg aaaaagagga agaggtcctg ctctgggtct
    35461 acgaggaaga ccttttccgg gtcctttcgg caaagcggga gaatgatttt ttccttcccg
    35521 gagtccgcct cgccgattcc ctccggttta gccattctct ggaagaagcc ctcgccgagc
    35581 gggagacggt cgtctgcgca gttccttccc acgcggtccg agaggttttt ctccgggcca
    35641 gaccccatct ctccaggaaa gctctcctta tcagtgtcac caaaggtctt gaggatggaa
    35701 cccacctgcc catgtcgcgg gttttgcagg aggtcatggg gtccggaacc aaggcggaga
    35761 ttgctgccct ttccggaccc agtttcgccg gagaggtatc tcgcaggatt cccactgccg
    35821 tggcagcagc gggaactact tttcgggctg cacagaaagc ccaggaccta ttttttcgtc
    35881 cctacttccg ggtctatacc aatccagacc tgatcggggt tcaattgggg ggagccatca
    35941 aaaacgtaat ggccatcgcc gctggcgcat cggacggaat gggtttcggc catagctcga
    36001 gggcagcgct catcacccgg gggttggcgg aaatgacccg cctgggggtg agcatggggg
    36061 cccagaggga gacctttttc ggcctcgccg gattggggga cctagtcctc acctgtactg
    36121 gagacctgag ccggaatcga caggttggcc tagagctggg aagggggaaa gcgttgcaag
    36181 agatccttgg gggcatgcgc atggtcgccg aaggagttcg cacggccaag gctttgtggg
    36241 aattggcccg aatccaaaag gtcgaaatgc ccatcgccca aaatgtctat gagatccttt
    36301 accaagagaa aggcccccgg gaggccgttc aagagttaat gtcccgccta ccccgctcgg
    36361 aacaagaaga ccttcccgtg gattcctccc atttttaatt ttcggcttct ctgaacctgg
    36421 aaaaggcccg cctatttcgt cgacggggga gagacaggag ggaggcttat cagcagtctt
    36481 tcagcgagct tccgcccata gatttcggcg gaatcacggg aggcatggat ggttcccgcg
    36541 gacatccaca ccagttcgcc tttttccgac tcgaaaatgc gcagaatcag ccggacttgt
    36601 gagcttcccc cgaagtaggt tggaagcacc tggtcggtga aggattcgcc gtcccatgac
    36661 tgaacggatt ggcttccgag gcgggatttt tgcaccctgt attccggaag agaagcgatc
    36721 ataataagtc tggccttcaa ccgttcagcg acctttttca aagagtcccg gtcggaaaga
    36781 ataaggaggg cggtcaggcg catctcttcg agagtgctta cgacctttat ttcctccact
    36841 aggtggtaac cctttttctc cagagaatca cggatgatgg ggtaaaggtt cgaacccgaa
    36901 gccgggtggc cggtggcatc ttggatcggc agcagggcca acggttgata ttcagcaaga
    36961 tcgaccttgg gtgataccgg cggttccaga ttcacccggt aaaggcacgc actcagcagg
    37021 atgaggaaaa tggccgccag gaccggggct gagtattttt gtagcctcag gagttccctc
    37081 aagaacttgc tacttaagga aaaattcgct aaaattacgt taattgacct catgaagaat
    37141 tcctaaaagg tctcttgggt cgtcaccccg gcgaaagccg ggtccaaaac ctcttgagat
    37201 aactagattc cggcttttgc gggaatgaat tctaaggaga ttcgattttc tacgcaccga
    37261 tcaactcata gtattagacc gcacaaaaaa acccctgtca agcgagcgat gaaccgatat
    37321 gcccatctat gagtatcgtt gtcaggaatg tggaaaggtt tccagctacc tggtgatgaa
    37381 tatccgcgag gcccatcacc cgcagtgcaa gcgctgccag agtggaaaaa tgaccaggct
    37441 catctcccgg gtggcgcgga ttcgttctga agaaagccgt ctggaatcca tggccgaccc
    37501 cagcaaactg ggaggcttgg atgaaaatga ccccgccagc atggctcgct ggatgaagcg
    37561 tatgggaaaa gagatggggg aagacctggg cgaagatgta gattccatga ttgatcaggc
    37621 catggaagaa gagatggccg gcaagggagc ggagtcagga agcaaggatg aagagctata
    37681 gtcataatcc ctagattcca actcccagat tttggaaagg aagagcatga ttgcttcatt
    37741 cctgaaggat ttgcagggaa catagaaaga gggaatatgc ccatcgctgg aaacccgaaa
    37801 aagctagccc aggacgtggc caatggattt cagcagttca cccaagcctc tctccgtcca
    37861 tataccctag aagacctgaa a
//
LOCUS       DASPRR010000171         9467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_529491, whole genome shotgun sequence.
ACCESSION   DASPRR010000171 DASPRR010000000
VERSION     DASPRR010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529491"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VEL68_06760"
     CDS             complement(<1..394)
                     /locus_tag="VEL68_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXZ35700.1"
                     /translation="MKMQDGSLFRFFIFAFLVSLVTLILPRPTSAQDYPSKPITIYCG
                     YEAGATTDLTARALGAGLEKLLGVPVVVENKAGGGATVAAGLVASRKPDGYTLGVVST
                     GVITVRPHIFKVSYDPFKDFTLIAQYSRY"
     gene            complement(546..2060)
                     /locus_tag="VEL68_06765"
     CDS             complement(546..2060)
                     /locus_tag="VEL68_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ35701.1"
                     /translation="MDFFSNFIAGFAVALQPINLAFCFLGVFIGTLIGVLPGIGPVGT
                     MAILLPVTYGIPPTTAIIMLAGIYYGAQYGGSTTSILVNIPGEAASVITCLDGYQMAL
                     KGRAGPALGIAAFGSFIAGTIGVIGLQLMAPPLVSVALRFGPPEYFSLMLLGFVILTY
                     LAQKSMIKALMTAGVGVVLGTIGLDTMTGMPRFTFNIPELLDGVGLAPLAMGLFGISE
                     ILLNVEKKIKLELLTTKVRNLFPNLEDWRRSIWAILRGTGSGFFLGILPGGGAVLGSF
                     VSYALEKRVSRHPEEFGKGAIEGVAAPESANNAAAQGAFIPLLTLGIPANVVMAILLG
                     ALMIHNITPGPMLVKEHPQLFWGVISSMYLGNIMLLVLNLPLIGLWVQLLRVPYSLLF
                     PLILFICLIGAYVINNSAVDVAIMFIFGVLGYFMRKFDYEPAPMVLAYVLTPLLENAL
                     RQSLILSDGSFAIFMSRPISAGCLITAAALLFSSLLPMIRKKRETLVAEAEKDE"
     gene            complement(2081..2539)
                     /locus_tag="VEL68_06770"
     CDS             complement(2081..2539)
                     /locus_tag="VEL68_06770"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ35702.1"
                     /translation="MNTDRVSSLFWLAVGLATLYGSVQLGLGTLREPGSGLFPFLAGV
                     FISLVALIIFLKSLIQWRAVPLDLKAHWTGVNWHRSLIICLLVLGFILFLEGLGFFVT
                     SFLLLFLIFRYVERLSWGNSIIIPVLTLGLTYLLFNVLLKSTLPKGIFGI"
     gene            complement(2618..3415)
                     /locus_tag="VEL68_06775"
     CDS             complement(2618..3415)
                     /locus_tag="VEL68_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ35703.1"
                     /translation="MEENLLIVEKKERVCTVIFNRPEKRNALTPLMLFQLADLLKSIQ
                     AEGEIRCLVLRGAGDKAFTAGYDISDIPRNMTPEMAAAFKKKNPLQTGLQSIIDFPYP
                     VIAMLNGHAFGAGCDLAMTCDLRIAAENAQMGLPPAKLGIIYQPNGILRMINIVGLAS
                     AKEIFFTGRSYAAPKAKEMGLVNYVLPLDQLASFTYEMAREISENAPLSLKGMKVIFN
                     KIFLSQKLDPQDTEEIEALRLQAFNSEDIKEGQQAFKEKRKPVFKGK"
     gene            4205..5746
                     /locus_tag="VEL68_06780"
     CDS             4205..5746
                     /locus_tag="VEL68_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ35704.1"
                     /translation="MSTLFQPFQLGRLELRNRMIMPSMVTLLASESGGVTQRMIDYYA
                     ERAKGGVSLITVESAYVMEKDREMGRLGIENPQLQVGLNELAESIQERGARAFLQLNH
                     RGSVLSIRPGKGPDQLSPEEIENIIELFSEAARKAQKIGFDGVEIHGANVYLIAEFLS
                     PLTNHRKDRYGGSLENRLNFPVDILRRVRKKVGVDFPVNFRMVGHQYADGGLVLEDTK
                     IIAQRLEEAGASALHVSAGSPPAPYWHTPPMAVPRGCHAELAGAIKKTVRIPVIAVGR
                     INDPLLADRILEEGKADLVAMGRALIADPELPLKAQEGRLEEVRKCLACNYCRRRIVE
                     LNRTLRCAVNAQAGRERDSRVFPAARSRKVMVIGGGPAGMEAARVLAWRGHRVAMYEK
                     KAKLGGQVNFAAIPPHKEELRTLLDFLVVQLERLKVQTHCGAQATAETIAQEKPEAVI
                     VATGARPLIPEIPGVPEEIIFTPEEALEPNRPIGEKIVVIGGGNGGVRGGGVPRQQGE
                     AGHHR"
     gene            5685..6068
                     /locus_tag="VEL68_06785"
     CDS             5685..6068
                     /locus_tag="VEL68_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ35705.1"
                     /translation="MVGCEVAEYLASKGKRVTIVEKLPEIALGMEGHTRRLLLERLNL
                     LSVRVFTEAEVISLEGRTAILRQPGKSMEIEAEGFVAAMGAGADRSCEEFQSCGLPFY
                     AVGDCAGVRDIAAAIHEGFAAAMGI"
     gene            6111..7610
                     /locus_tag="VEL68_06790"
     CDS             6111..7610
                     /locus_tag="VEL68_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ35706.1"
                     /translation="MELLHNLVLGFSVLLHPSNLFACFLGVLIGTLVGVLPGLGPAAA
                     ISLLLPTTYKLSPLTGIIMLAGIYYGAMYGGSTTSILVNIPGESASVVTCLDGYQMAR
                     QGRAGPALGIAAFGSFIAGTFSIVALMLVAPPLARMALKFGPPEYFSLMLLGLMILSY
                     LSTGSMVKAWMMAALGLFIGTIGQDRMVGTLRFTYQLDVLADGVGLVPVVMGLFGITE
                     VLVNIEQSVTRQIFKTHLSHLLPSRQDWARSILPILRGSALGFLLGILPGGGAIISSF
                     ASYAVEKKFSRTPQKFGTGMIEGVAGPESANNAATGGAFVPLLTMGIPSNAIMALLLG
                     ALLVQGVKPGPFFVKEYPDLFWGVISSMYVGNGMLLVLNLPLIGLWVKILKLPYPLLF
                     PLIVLFCLIGVFSLNNTAADIYVMGFFGFLGYLMKKFEYDGAPLVLALVLSPMIEDAF
                     RQSLLMADGSFLIFFSRPVSLVLMGVAVLIMASSLLPWLKRRPAEGLEE"
     gene            7771..8760
                     /locus_tag="VEL68_06795"
     CDS             7771..8760
                     /locus_tag="VEL68_06795"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ35707.1"
                     /translation="MAMQPRFRTPGRVFWGGLILLALCGGSAGATEKDYPNKMITLIN
                     PYAPGGGIDFAYRGIVDILPEYLGQKIVVSHKPGAVGSIGTAAAAKAKPDGYTLLAGS
                     TSHVNLVPATRKVPYTPADFVSVGTFAKAISVLSVEKDAPWKTLAEFVADAKKNPGKY
                     KFSTIGLMSAHHFCTELFMRAAGIQMVHIPYNSDTLAITATLGGHSHVCQTTVQPTLP
                     HLQSGALRALAVSDVTRYPYLPDVPTFKELGYNVEMIIWFGMLAPKGTPKEVIDRLYA
                     AQKKAFDENKLQPIIEKIGMIPFLTSPQEMDRIVQENQERFTRIAKEANLEVK"
     gene            8806..9261
                     /locus_tag="VEL68_06800"
     CDS             8806..9261
                     /locus_tag="VEL68_06800"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ35708.1"
                     /translation="MKRLDFLSSGFWLMVGVLICEESWRINLGEFHNPGPGFLPFGAG
                     LILAGGALAVLAKALRSGPGREGAFWADRRRWPKVFLTLGCIIFYGLFLESLGFLLTT
                     FLSMGFLFRVIEPQRWRTVLMGALFSAVGAYLIFQTWLQVELPKGFLGI"
BASE COUNT         2003 a         2633 c         2852 g         1979 t
ORIGIN      
        1 tgtagcggct gtactgggcg atcaaagtaa aatccttgaa ggggtcgtag gacactttga
       61 aaatatgcgg ccggacggta atgactcctg tggagacgac tcccagggta tacccgtcgg
      121 gcttcctgct ggccaccagg ccggccgcca ccgtggctcc ccctccggcc ttgttttcca
      181 ccaccaccgg gacgccgagg agtttctcca gaccagcgcc cagtgcccgg gcggtcaaat
      241 cggtggtggc ccctgcctcg tacccgcagt agatggtgat gggcttggac ggataatcct
      301 gggccgaggt cggcctgggc aggatcaggg ttacgagtga aaccaggaag gcgaaaataa
      361 agaatcggaa aagagaacca tcctgcattt tcatgttgac ctccccccag actgcctgga
      421 attcattttc ccctgaccat tattttatcc gttcttcggc ttttagagca agttttgctg
      481 aaaccgcttg gcttcgccct tcgtatcttc tgggcatcgc ccgattcttg tacccccgcc
      541 ccgcttcatt catctttttc cgcctccgcc accagggtct cccgtttctt gcggatcatg
      601 gggaggagcg aagagaacaa aagggcggcc gcggtgatca ggcatccggc ggagatgggg
      661 cggctcatga agatagcgaa gctgccgtcg gagaggatca gggactggcg cagcgcgttt
      721 tccaagagag gggtcaaaac gtaggccaat accatgggcg ccggctcata gtcgaacttg
      781 cgcatgaagt aacccagcac tccgaagatg aacatgatcg ccacatcgac cgcgctgttg
      841 ttgatcacgt aagcaccgat gagacagata aaaagaatca gcgggaagag aagggaataa
      901 gggacccgca ggagctggac ccagaggccg ataagcggca ggttcaggac caggagcatg
      961 atattcccca ggtacatgct gctgatgacc ccccagaaga gctggggatg ctcctttacc
     1021 aacatgggcc cgggggtgat gttgtggatc atgagcgcgc ccagcaatat ggccataacc
     1081 acgttggccg ggattcccag ggtgaggagg ggaataaaag ccccctgggc ggcggcattg
     1141 ttggccgact cgggggcggc gaccccctcg atcgccccct tgccgaattc ttcgggatgc
     1201 cgggaaacgc gtttttccaa agcataagac acgaaggagc cgagaacggc tccccccccg
     1261 gggagaattc ccaggaagaa tccggaacca gtgccccgga gaatagccca gatggaccgc
     1321 ctccaatcct ccaggttagg aaacaaattc ctgaccttgg tggtcagaag ctccagtttg
     1381 atcttctttt ccacattcag caggatctcg gagatgccga agagtcccat ggccaggggg
     1441 gcgagtccta cgccgtcgag cagctccggg atgttgaagg tgaagcgggg cattccggtc
     1501 atggtgtcca gcccgatggt ccccaggaca acccccacac cggcggtcat caaagctttg
     1561 atcatcgact tctgggccaa gtaggtaagg atcacaaagc ccagaagcat cagggaaaaa
     1621 tactcggggg gaccgaatcg cagggccacg gagaccagtg gaggggccat gagctgcagg
     1681 ccgatgaccc cgatggtccc ggcgataaag gagccgaagg cggcgattcc cagggcgggg
     1741 ccggcccggc cttttagagc catctggtag ccatccaggc aggtaatcac cgatgccgct
     1801 tctcctggaa tattgaccag gatggaagtg gtcgaacctc cgtattgggc cccgtagtaa
     1861 atcccggcca gcatgatgat ggcggtggtg ggcgggatcc cgtaggtcac cggaagaagg
     1921 atggccatgg ttcccaccgg cccgattcct gggagaacgc cgatgagggt tccgatgaaa
     1981 acccccaaaa aacagaaggc gaggttgatg ggctgcaggg ccacggcaaa accggcgatg
     2041 aaattggaga aaaaatccat aaccaaaagt cccttccgga ttagatgccg aagatcccct
     2101 tgggtaaggt ggatttgaga agaacattga aaagaagata ggtaagaccc agggtgagga
     2161 cgggaatgat gatggagtta ccccaggaaa gcctctccac atatcggaag attaggaaga
     2221 gaagaagaaa actggtgacg aagaacccca gtccttccag aaaaaggata aagcccagga
     2281 cgaggaggca gatgataagg gaacgatgcc agttcacccc ggtccagtgg gctttgaggt
     2341 ccaatggtac ggcccgccac tggatgaggg atttcaggaa tatgatcagg gccaccaggg
     2401 agatgaaaac cccggcgaga aaaggaaata atcccgaacc gggctcccgc agggttccca
     2461 ggcccaactg aaccgatccg tagagggtgg ccagcccaac ggcgagccag aagagactgc
     2521 ttaccctgtc cgtattcaaa agttctttcc tcctttcaga agcctcggcg caggggcgac
     2581 cggacttcgg cgagctcagt cgagccgcca gtggccccta tttcccttta aacaccggtt
     2641 tccgtttttc cttgaaggcc tgctggcctt ccttgatgtc ttcgctgttg aatgcctgaa
     2701 ggcgaagggc ttcaatctcc tcggtgtcct gggggtccag tttctgggaa aggaagattt
     2761 tgttgaaaat gaccttcatt cctttcaggg acaggggagc attctcggag atttcgcggg
     2821 ccatctcgta ggtaaaggaa gcgagctgat ccaggggaag gacgtaattg accaaaccca
     2881 tctctttggc cttgggggct gcgtagctgc gtccggtgaa aaaaatctcc ttggcactgg
     2941 ccagccccac gatgttgatc atcctcagga ttccattggg ctggtagatg atccccagtt
     3001 tggccggcgg aagccccatc tgggcgtttt ccgcggcgat gcgcaggtcg caggtcatgg
     3061 ccaggtcgca cccggccccg aaggcatggc cgttcagcat ggcgatgacc gggtagggaa
     3121 agtcgatgat cgattgcagc ccggtttgca ggggattttt ctttttaaag gcggcggcca
     3181 tctccggggt catgtttctg ggaatgtcgg agatgtcgta accggcagtg aaggccttgt
     3241 cccccgctcc ccggaggacc agacagcgga tttctccctc ggcctggatg gatttcagaa
     3301 ggtcggccag ttggaagagc atgagcggag taagggcatt gcgcttctcc ggccggttga
     3361 agatgaccgt gcagactctc tctttctttt ccacgatcaa taaattttct tccatgggat
     3421 ctcctattcg gattgtgtcc tgcaggaagg aatcctctct ctattacgaa gactcctggg
     3481 gcagaggcgg gtcatgaata aaaccttggc aacccgctcg tcgcaactca agttataaaa
     3541 gccccatcaa tctgtcaagc gatttctcga aagaatcgga aaaacaaaat ccatctcgac
     3601 aaaaagcccc acttttggta gaggttagtc agctaaattc ccttaggtga actctcgggt
     3661 tgccggtgat ccgcctgtgg cggattgaca tttccaggat gcttgggttg tggcccgggg
     3721 gggaggggcc tttgggtaaa gagtatcagc ccattcgatc tcggctttca aatttggcat
     3781 tgggcttttc catttaaccc atgttgacat ttccagcccg aaaaccccct ctccccttcg
     3841 tgggagaggg tcggggtgag ggggattgaa gcagaagcag gagattccga agacttttca
     3901 ccccaaccct tccctccccc atcgaggggg agggagaatc aacgctgaag aacctcaagg
     3961 gtcaagcgga aaatcgttct ttgtatcagc caaccgtatc cgccggattt ttgaaaagtg
     4021 ggccgaacga acatgagtca agtggaaaag cccatatggc atttcggatt tcatgttctt
     4081 caatgagcga tctgcattct gcaatttcga gggcggttca cgatttttca aggctccggc
     4141 tctcccggag gtatatgact catcttggag agaaaaaatg ttttttcagg ggaaatccag
     4201 agttttgagc actctgttcc agcccttcca gctcggaagg ctggaactgc gcaaccgcat
     4261 gattatgccg tccatggtca ctcttctggc aagcgagtcc gggggggtaa cccagagaat
     4321 gatcgattat tacgcggaga gggccaaggg cggagtaagc ctgatcaccg ttgaatccgc
     4381 ctatgtgatg gaaaaggatc gagaaatggg gcgcctcggg atcgagaatc cccagctcca
     4441 ggtgggcctc aacgaattgg ccgaatcgat ccaggagcgg ggggccagag cctttcttca
     4501 gctcaaccat cgggggagcg tcctcagtat tcgcccggga aaaggaccgg atcagctgag
     4561 cccggaggag attgaaaaca tcatcgagct tttttcggag gcagcccgga aggcccaaaa
     4621 gattggattc gacggggtgg agatccacgg ggccaatgtc tacctgatag ctgaattcct
     4681 gtctcccttg accaaccatc gaaaggaccg ctatgggggg agcctggaaa atcggctgaa
     4741 ttttcccgtg gatattctcc gccgggtgcg aaaaaaagtg ggggtggatt ttccggtcaa
     4801 ttttcggatg gtgggccatc aatatgcgga tggcgggctc gtcctggagg atacgaaaat
     4861 catcgcccag cgcctggagg aggccggagc aagtgccttg cacgtgtccg cagggagccc
     4921 cccggcccct tattggcaca ctcctcccat ggcggtcccg cgggggtgtc acgcggaact
     4981 ggccggggcg atcaagaaaa cggtccgcat tccggtcatc gccgtcggtc ggatcaacga
     5041 ccccctcttg gcggatcgga ttctagaaga aggaaaagcc gacctggtgg ccatggggcg
     5101 ggccttgatc gccgatcccg aacttcctct gaaggctcag gaagggaggt tggaggaggt
     5161 ccgcaaatgt ctggcctgca actattgccg gaggcggatc gtcgaattga accggactct
     5221 gcggtgcgcg gtgaatgccc aggcggggcg cgaacgggac tcccgggtct tcccggccgc
     5281 gcggagccgg aaagtcatgg tgatcggagg gggccccgcc ggcatggagg ccgcgcgggt
     5341 cttggcttgg cgcggtcacc gggtggcgat gtatgaaaag aaggccaaat tgggaggcca
     5401 ggtcaacttt gcggcgatcc cgccccacaa agaagagttg cgcaccctcc tcgattttct
     5461 cgtcgtccag ctggaaaggt tgaaggtgca aacccattgc ggagcgcaag ccacggcgga
     5521 gacgatcgcc caggagaagc ccgaggcggt catcgtggcc acgggcgctc gccccctgat
     5581 cccggagatt cccggtgtac cggaggaaat aatcttcacc cccgaagaag ccctggaacc
     5641 caatcgaccg atcggagaaa agatcgtggt catcgggggg gggaatggtg gggtgcgagg
     5701 tggcggagta cctcgccagc aaggggaagc gggtcaccat cgttgagaaa ctgccggaga
     5761 tcgccctcgg gatggagggc catacccgaa ggctgctcct ggagaggttg aacctccttt
     5821 ccgtacgggt tttcacggag gctgaagtga tctccctcga ggggagaacg gccatccttc
     5881 gccagccggg aaagtcgatg gaaatagaag ccgaggggtt cgtagcggcc atgggggccg
     5941 gtgccgaccg aagctgcgag gagtttcagt cctgcggcct gcctttttat gccgtgggcg
     6001 actgcgccgg ggtgcgggat atcgccgcgg cgatccacga aggctttgcc gcggccatgg
     6061 gaatctgatc cgagtccggc taggggagaa agagcggagc ggaacttttc atggagctgt
     6121 tgcataatct agtcctgggc ttttccgttc tcctgcaccc ctccaacctc ttcgcctgct
     6181 tcctgggggt gctcatcgga acgctcgtcg gggtcctccc gggcctgggg ccggccgcgg
     6241 ccatctccct cctcctgccc accacctata aactttcccc cctgaccggc atcatcatgc
     6301 tggcagggat ctactatggc gccatgtacg ggggttccac cacctcgatc ctggtcaaca
     6361 tccccggcga gtcggcctcg gtggtcacct gtctggacgg ataccagatg gcgcgccagg
     6421 gaagggccgg gccggcgctg ggcattgcgg ccttcggctc cttcatcgcc ggcacgttca
     6481 gtatcgtagc cctgatgctc gtggccccgc cgctggcccg catggccttg aaattcggcc
     6541 cgccggagta tttttctctg atgctcctgg ggctgatgat tctttcctat ctctccaccg
     6601 ggtccatggt caaagcctgg atgatggccg ccctggggct cttcatcggc acgatcggcc
     6661 aggaccgcat ggtgggcacg ctgcgcttta cctatcaact cgatgtcctc gcggacggcg
     6721 tggggctggt gccggtggtc atgggcctct tcgggattac cgaagtcctg gtcaacatcg
     6781 aacagtcggt gacccggcag atttttaaga cccatctttc ccacctcctg cccagccggc
     6841 aggactgggc caggtccatc ctgcccattc tgcgtggctc ggctctgggc tttctgctgg
     6901 ggattctgcc cggagggggg gcgatcatct cttcctttgc ctcctacgcc gtggaaaaaa
     6961 agttctcccg gactccgcaa aaatttggca ccgggatgat cgaaggggtc gccggcccgg
     7021 aatccgccaa caacgccgcc acgggcggcg ccttcgtccc cctgctgacc atgggcattc
     7081 cttccaacgc aattatggct ttgctcctgg gtgcgctcct cgtccagggg gtgaagccgg
     7141 ggcccttctt cgtaaaagag tacccggacc tcttctgggg ggtgatcagc agcatgtatg
     7201 tggggaatgg catgctcctg gtcctgaacc tgcccctcat agggctgtgg gtgaaaattc
     7261 tcaagctccc ctatccgctc ctcttccccc tcatcgtcct cttctgcctg atcggagtct
     7321 tcagcctgaa taatacggcg gccgatattt acgtgatggg cttcttcggg ttcctaggct
     7381 acctgatgaa gaagtttgaa tacgacggtg cccccctggt gctggctttg gtcctgagcc
     7441 ccatgatcga ggatgctttc cgccagtccc tattgatggc ggatggaagt tttctcatct
     7501 ttttctcgag gccagtttct ctggttctga tgggcgtggc tgtcctgatc atggcctcca
     7561 gcctcctgcc ctggcttaaa cgccgcccgg ctgaggggct ggaggagtga gctgagcgag
     7621 aaccaaaaga agatattgta ggggcggccc cctgtggccg cccgcaagat gggcaaccac
     7681 agggggttgc ccctacaatt tgtctccatt ttgagattaa tttggctggt aaaggactta
     7741 agagaactgg gaggagaaag gagatttctc atggccatgc agccaagatt tcggactccg
     7801 ggtcgggttt tttggggagg gctgatcctt ctggccctct gtggggggtc ggccggcgcg
     7861 accgagaagg attacccgaa taagatgatc accctgatca atccctatgc tccgggaggg
     7921 gggattgact ttgcctatag gggcatcgta gatattctgc ccgaatatct cgggcagaaa
     7981 attgtcgtct cccacaagcc aggcgccgtg gggtcgatcg gaaccgctgc cgcagccaag
     8041 gccaaaccag acggctacac cctcctcgcc gggtcgacct cacacgtgaa cctggtcccg
     8101 gccacccgga aagtcccata tactcccgcc gattttgtct ccgtgggaac cttcgccaag
     8161 gccatcagcg tcctttcggt agaaaaagac gcgccgtgga aaaccctggc ggaattcgtg
     8221 gccgacgcca agaagaatcc cggcaagtac aaattctcca ccatcgggct catgtccgcc
     8281 catcacttct gcaccgagct tttcatgcgg gcggcgggaa tccagatggt ccatatcccc
     8341 tacaacagcg acaccctggc gatcaccgcc accctgggcg gtcatagcca tgtctgtcag
     8401 accaccgtcc agcccacgct tccccacctc cagtctgggg ctttgcgggc gctggctgtt
     8461 tcggatgtga cccgttatcc ttacttgccc gatgtcccta ctttcaaaga gctgggatac
     8521 aatgtagaga tgatcatctg gttcggcatg ctggcgccca aaggaacccc gaaggaagtc
     8581 atcgatcgac tttacgcggc ccagaagaag gctttcgacg agaataagct tcaacccatc
     8641 atcgaaaaga tcggcatgat ccccttcttg acctctccgc aggagatgga caggattgtc
     8701 caggagaatc aggagaggtt cacccggata gccaaagagg ccaatttgga agtgaaatag
     8761 ggccggcctc cgctgcgggg ggaaaaagaa aggcgcaagg agaaagtgaa gaggcttgat
     8821 ttcctcagct ctggtttctg gttgatggtc ggggtgctca tctgcgagga atcctggcgg
     8881 attaacctgg gggaattcca caacccaggc cctggattcc tgcctttcgg ggccggtctg
     8941 atcctggctg gcggtgcgct ggccgtcctg gcgaaggccc tgcggagcgg accgggacgg
     9001 gaaggagcat tctgggccga ccgcagacgc tggcccaagg ttttcctgac cctgggctgc
     9061 atcatttttt atggcctctt cctggaatcc ctggggtttc tcttgaccac ctttctcagc
     9121 atgggatttc tgttccgggt catcgagcct cagcgctgga ggacggtcct catgggcgcc
     9181 ctcttttcgg ctgtcggagc ctatctcatc tttcagacct ggctgcaggt agagttgccc
     9241 aagggttttc tggggatttg atacatttta gacgcttgag gcctcattaa taaaaatatg
     9301 ttggtaggaa gaaatccttc acaaggccgt agaggcacga tgaatcatgc ctctacaatt
     9361 ttcccctccc ctcgcgggag gggatcaagg ggagggggat gcattcccga ttcaccccca
     9421 cccttacccc ccctatcaag agactgtgtc gtaatccgtt catggtt
//
LOCUS       DASPRR010000172         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_529515, whole genome shotgun sequence.
ACCESSION   DASPRR010000172 DASPRR010000000
VERSION     DASPRR010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529515"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VEL68_06805"
     CDS             complement(<1..953)
                     /locus_tag="VEL68_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ35709.1"
                     /translation="MSTIVLVNPPYAFWSPEKNYLRPFIGNLPSLGLLSLGAILRKSG
                     YRVKIVESASRGLSFSQTIDQILQERPAYVGLSCTTASVENAAKIARAIKEARPDTRV
                     LAGGPHITALPEKTFRRFPDFDFGILGEGEAALVELLESLEGKKDLDQVESAVYRRGE
                     EILVNPRRRFIENLDTLPFPAFDLLPDFPRVYHPPFLNYQKGPAASLLSSRGCPQDCT
                     FCDRSVFGNRYRYYSEDYLFAGVSFLVRRYGIRHLVFTDDQFAASRPRLVRLCEKMSG
                     GGLNIQWNCDARVDSMDPDLLKVMKRAGCWMISYGIESGSQ"
     gene            1223..2131
                     /locus_tag="VEL68_06810"
     CDS             1223..2131
                     /locus_tag="VEL68_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ35710.1"
                     /translation="MRYLTPQTIHPRIEYLGRQEMNVYLLKGEEYMIIEGGMGYIVSD
                     MLRQFQERGINLNRITRLLILHSHFDHCGIVPFFKRRLPGLKIIGSRRSQELYRKEKV
                     IQFIRDRNREMIQFLKMEKEAAELNLDFDQLAVEEAVGEGDTIDLGEGVEVRVIEMPG
                     HSSCSIAAYVPSLRALFPSDAGGIPGEGKEIFPSGNEDFMLYQKSLEELQPLYVDVVC
                     LARNGAFLGQDAKEYVSRSVEEAEKMRLEIIRQLQGVPDPDEELARLARERYDSLKTR
                     GIPWDVYLGLMRGVVKKILESEKKSQ"
BASE COUNT          553 a          565 c          617 g          448 t
ORIGIN      
        1 ttctgcgaac cgctttcgat cccgtagctg atcatccagc atcccgccct tttcattacc
       61 ttcagcaggt ccgggtccat tgaatccacc cgggcgtcgc agttccactg gatgttcagg
      121 cccccgccgg acattttttc gcacaagcgc accaggcgag gccgggaagc ggcgaactga
      181 tcgtcggtaa agacaaggtg gcggattccg tatctccgga ccaggaaaga gacccctgcg
      241 aataagtagt cttcggaata gtaccggtac cggttgccga atacggatcg gtcgcagaaa
      301 gtgcaatcct gggggcaccc ccgggaagag agcagggaag cagcagggcc tttttgataa
      361 ttcagaaagg gtggatggta aacccgagga aagtcgggca agaggtcaaa cgcgggaaag
      421 ggcagggtgt ccaaattctc gatgaacctg cggcgggggt tgaccaggat ttcttctccc
      481 cggcgataaa cggcgctctc cacctgatcc aggtctttct tcccttccag agattccagc
      541 aattccacca gagcggcctc tccctccccc agaattccaa aatcgaaatc cgggaaacgg
      601 cggaaagtct tttcgggaag ggcggtgata tgggggccgc cggcgaggac ccgggtatcg
      661 ggtcttgcct ccttaattgc ccgggcgatc ttcgccgcat tctccaccga ggctgtggtg
      721 cagctcaggc ctacataggc aggcctctcc tgaagaattt ggtcaatcgt ttgggagaaa
      781 gacaaccccc gggaggcact ttccacgatt ttgacccgat aaccggattt ccgaagaatc
      841 gctccaaggc tcaggagccc cagagaagga agattgccga taaaggggcg gagataattc
      901 ttttccggag accagaaagc gtagggaggg ttgacgagga cgatcgtgct cataataagt
      961 tcattccatg tggggtgcac gctgccgcgc gcctgcatgg agttcggagt gagaacaaag
     1021 aaacattagc aaggaggacc gttcatttca agaaatatta ataccgccaa agttgctgga
     1081 agaaggggat tctggtaagg taggcgaaag cagaaaaatt tcaagcctca ggtttcagat
     1141 ttacatttcc ggttcggcct ttaaggcttt taactcgagg cccgaaaccc acaacccgaa
     1201 actgactttc ccggaggtct ccatgcgtta cctaaccccc caaaccatcc atccccggat
     1261 tgaatacctg gggaggcagg aaatgaacgt gtatctcctc aaaggagaag agtacatgat
     1321 catcgaggga gggatgggct atattgtgtc cgacatgcta agacagtttc aggagcgtgg
     1381 gattaacctg aacaggatca cccgcctgct gatccttcac tcccactttg accactgcgg
     1441 aatcgtgccc ttcttcaagc gcaggcttcc cggtcttaaa atcataggct ccaggcggag
     1501 ccaggaactg taccgtaagg agaaggtcat ccaatttatc cgggaccgga atcgggagat
     1561 gattcaattc ctcaagatgg agaaagaggc cgccgaactg aacctggact tcgatcagtt
     1621 agccgtcgaa gaagcagtag gggaaggaga taccatcgac ttgggggagg gggtggaggt
     1681 tcgggtcatc gaaatgccgg gtcacagttc ctgttccata gccgcctacg ttccctccct
     1741 gagggctctc ttcccttccg atgctggagg cattcccggg gaagggaaag agatcttccc
     1801 ctccggcaat gaagacttca tgctttacca gaagtcgctt gaagaactgc agcctctcta
     1861 cgtggatgtc gtttgtctcg cccgcaacgg cgcctttctg gggcaggatg ccaaagagta
     1921 tgtttcccgc tccgtcgagg aagccgaaaa gatgcgtctg gagatcatcc gccagcttca
     1981 aggggtaccg gacccggatg aagaattagc ccgactggct cgagagcggt atgacagtct
     2041 aaaaaccagg ggcatcccct gggatgtcta cctggggcta atgcggggag tagtgaaaaa
     2101 aatcttggaa agcgaaaaaa agagccaatg aaaaatcagc tgtcagcggt cagctctcag
     2161 cagaaaaaaa caactttaga aaa
//
LOCUS       DASPRR010000173         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_197442, whole genome shotgun sequence.
ACCESSION   DASPRR010000173 DASPRR010000000
VERSION     DASPRR010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197442"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1930
                     /locus_tag="VEL68_06815"
     CDS             206..1930
                     /locus_tag="VEL68_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ35711.1"
                     /translation="MTFNRRVFFYLFFLSLAAASCSSAKSFLRVENPPRSDYLPPGPQ
                     AEAYYHFLIGQKYQQAGQMDKALQQFQQALEKDPASPYLHTNLAAIYLKQGATEKALL
                     QAEKAIQADPKYIQAYLLLGGLYLSTNRRDKAIQTYQTILQIDPKSQEALLFLGTINA
                     EAKNYDSAISYLKTLLEIDPQSLMGHYYLARIYAEMKRFDQAKTHYQSALAIRPQFEP
                     ALLDLALIHEIQQENEKAIQILKDILDLNPANAEAHERLGQVYLRQKNLSGALNEFQA
                     VEKLTIQNQEVRTKIGLIHFEQGQLDEAISQFSLVLSSQPTNYRVRYYLASAYEEKKE
                     WDRALEEFKKIPSDSTFYVDSQVQQAFILEKQEKVQEAIQVLKAAIKVKPQNADLYRF
                     LASLYEKTSQYEEGIRLLKEALSFAPKDEKIYFSLGVLYDKAGNWQECIVQMRKALEV
                     KPDHPQALNYIGYTYAERGIHLDEAEALIRKALELKSDDGYITDSLGWVYFKKGEFAK
                     AVAELEKAHRLAPEDPVIAEHLGDAYAKQNLTAKAIQMYERALQLDPKKTEVQEKIKK
                     LRQEKKGP"
     gene            1927..2703
                     /locus_tag="VEL68_06820"
     CDS             1927..2703
                     /locus_tag="VEL68_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HXZ35712.1"
                     /translation="MNPGLHLVLLLAAFLFSACATLPPLPAVPPPAEEVYQQVISRQG
                     ALEGLKGLAQVRVISPGRSFHGQQVIFARRPAFLRVESLSPVGTPLLYLVTDGKEIRL
                     YNPEENRYYQGDYRPGSLSFALPMDLHPSEVVNFLLGGGPLPDPDQISLRPDSSEGLW
                     VLELHSSSRGVTETLWVHPQSFQVLRADLHRSSLSYRISFSDFRQAKEKLFPHRMRLS
                     SEDSRTRISVVFPEVELNPDWGLQDFTLPLPQGATVHPLP"
BASE COUNT          671 a          891 c          765 g          637 t
ORIGIN      
        1 caccgcctca ggatgatgat ccaaatttct ccgccctgaa ccccatgaga tccccacctc
       61 ctttccagag atctaatttt cgaaggactc ttttttaaga actgggggtg ggcaaggctc
      121 attccccatt tgacaacccc gcgtttcgaa agtagaataa aattaggtgc gggcagattt
      181 tttcgcaact tcgaggattt cccttatgac cttcaaccga agggtttttt tttatctctt
      241 ctttctttct ttggccgccg cctcttgctc ttccgccaag tccttcctga gggtcgagaa
      301 tccgccccgt tcggattatc tgcccccggg tccgcaggct gaggcctatt atcatttcct
      361 cataggccaa aagtaccagc aggcgggcca gatggacaag gcccttcagc aattccaaca
      421 ggctctggaa aaggaccctg catcccccta tttgcacacc aacctggcgg ccatttatct
      481 gaagcagggc gctacggaga aggctttgct tcaggcggaa aaagcgatcc aagccgatcc
      541 caagtacatt caggcgtatc tgctcctggg agggctttat ctctctacca accggcggga
      601 caaggctatt caaacctacc agaccattct gcaaatcgat cccaagagcc aggaggccct
      661 cctcttcctt ggaacgatta atgccgaggc caaaaactat gactcggcga tctcttatct
      721 gaagaccctg ctggaaatcg atcctcaatc cctgatgggc cactattacc tcgcccggat
      781 ttacgccgag atgaagcggt ttgaccaagc caagacccat taccagtcgg ccctggccat
      841 ccggccccaa tttgaacccg ccctcctgga cctggccctc atccatgaga ttcagcagga
      901 gaacgaaaaa gccatccaaa tcttgaagga tatccttgat ttgaatccgg ccaacgcgga
      961 ggcccatgaa cgtctggggc aggtctatct ccgccagaaa aacctcagcg gggccttgaa
     1021 cgaattccag gccgtggaaa agctgaccat tcaaaaccag gaagtacgga ccaaaatcgg
     1081 attgatccac ttcgagcagg gccagctgga cgaggccatt agccaatttt ccctcgtcct
     1141 ctcttctcaa cccacaaact accgggttcg ttactacttg gcctcggctt atgaagagaa
     1201 gaaggagtgg gatcgggccc tggaggaatt caagaagatc ccctccgatt cgacttttta
     1261 tgtggacagc caggtccagc aggccttcat cctggagaag caggaaaagg tccaggaagc
     1321 catccaggtc ctgaaggcgg ccatcaaagt caaaccgcaa aacgccgacc tgtaccgttt
     1381 tctcgcttcc ctgtatgaaa agaccagcca gtacgaagaa ggcatccgtc tgctcaagga
     1441 ggctctctcc ttcgccccga aggatgaaaa gatttatttc agtctgggag tcctgtacga
     1501 caaggccggc aactggcagg aatgcatcgt gcagatgcgc aaagccctgg aggtcaagcc
     1561 cgatcatccg caggccttga actatatcgg ctatacctac gcggaacggg ggatccatct
     1621 ggatgaagcg gaggcgctca tcaggaaggc tctggagctg aagtcggatg acgggtacat
     1681 caccgacagc ctgggctggg tctacttcaa aaaaggggaa ttcgcgaagg ccgtagccga
     1741 gctggaaaag gcccacaggc tggctcccga agaccccgtc attgccgaac acctgggaga
     1801 cgcctacgcc aaacagaacc taaccgccaa ggctatccag atgtacgaac gggccttgca
     1861 gctcgacccc aagaagaccg aggtgcagga aaagatcaag aagctccggc aggagaaaaa
     1921 agggccgtga atccgggcct ccaccttgtc ctcctgctgg cagcctttct gttcagcgcc
     1981 tgtgccaccc ttcctcccct tcctgccgtc ccccctcctg cggaagaggt ctatcagcag
     2041 gtgatatccc gccagggggc ccttgagggt ttaaagggcc tggcccaagt cagagttata
     2101 tccccgggaa ggtccttcca tggccagcag gtcatctttg cccgccggcc cgcctttctc
     2161 cgggtggaga gcttgagtcc cgtgggaacc cctctccttt acctggtaac cgacggaaaa
     2221 gaaattcgcc tttacaaccc tgaagagaac cggtattacc agggtgacta ccggcccggc
     2281 tctctatcct tcgccctccc gatggacctg catccgtcgg aggtggtgaa ttttcttctg
     2341 ggcggaggtc ccctgcccga cccagatcag atatcactcc gcccggattc cagcgaaggc
     2401 ctttgggttc tggaactcca ctcctcttcg cggggagtca ccgagaccct ttgggtccac
     2461 ccccaatcct ttcaggtcct ccgggcggac ctccaccggt cctccttgtc ttaccgaatc
     2521 tctttttccg atttccgcca ggccaaggaa aaactttttc cccatcggat gcggctctct
     2581 tccgaggact cccggacccg aatctccgta gtatttcccg aagtcgagct caacccggac
     2641 tggggactcc aggactttac tcttcccttg ccccaggggg caacggtcca tcctctccca
     2701 taagcaaaaa aggcgaaggg ctgggggcgg gcaggttgtt gctggttgta ggttccccga
     2761 tcctgattgg gcaagcggac ggtcgcgggt gatcagaaaa gaaccacgag agatgggact
     2821 aaccgatttc ttgaagtttc ttgcgggccc ggttggcctg gtcggactgg gggaagcggc
     2881 ggacgacctc tttcaggatc aatttggcgt tggtcgtatc tttcatctcc agaaaagcca
     2941 ttccctggcg gtagagggcg tcgg
//
LOCUS       DASPRR010000174         6961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1168574, whole genome shotgun sequence.
ACCESSION   DASPRR010000174 DASPRR010000000
VERSION     DASPRR010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168574"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1202)
                     /locus_tag="VEL68_06825"
     CDS             complement(330..1202)
                     /locus_tag="VEL68_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35713.1"
                     /translation="MMDTQLLISGVIFGLSIGSIFFLMAIGLSLCFGLMRIISLDQLL
                     YYSVGAFISYSIFVATGNTWFGMFGGMVAGGLVSFGVERFVYLRIYERDITFTMIASF
                     AILIAGVGAIKYIWGLVPRPAPIPLETQVIILGTAIPVYRLMVIVISFLVYFGIWLFL
                     NRTITGKAIRAGIEDVEHVEGLGINVYKLFTITFILAGTLSGLSGALHAPLIMVDPQM
                     GLHVLAFVFMVVIIGGLGSIKGTLASAFIVGQVISLGSLIYAPLAQMAPFAIMLLILL
                     IKPTGLYGSKLLKR"
     gene            complement(1199..2185)
                     /locus_tag="VEL68_06830"
     CDS             complement(1199..2185)
                     /locus_tag="VEL68_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35714.1"
                     /translation="MTNIFRWWWDILLAFSLLALLRVFSPLPFAIEIIIFSIYTMGCN
                     FLLGRVGFISFGQPAYLAVGAYATAFYLFYFGQNPYIGILLGLLIGAAVAAFVGPFFV
                     RLRSDYFALVNLALAVIVFYLMEKVLADITHGDNGLWFLTNMSSTPVLDLTRPKEFFI
                     FAFLVAMAIWAFYKYLDDSVYGACCLATKINEDKLKFLGYSNFNIRYLAFIIANTTTA
                     LAGSLYAVYLGFVSPEISSPARCADPVVVTILGGVGTLYGPIVGAIAYTGMKDLISGF
                     IANWELFIGLLLVFIMLAGEKGIWSTLEPVLKKALSRRGILPSVQDERGESR"
     gene            complement(2434..3126)
                     /locus_tag="VEL68_06835"
     CDS             complement(2434..3126)
                     /locus_tag="VEL68_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35715.1"
                     /translation="MNDGEILAVQGLNVSYGESKVLFDAGLRVKPGQVVTCVGRNGAG
                     KTTLLKTIAGFLKPTSGSIIFNDTNLVGLMSYDIARMGLKYIPQDKKVFSDLTVRENL
                     ELGSYATKDYNWDQVTDHFPRLKSLMDRKGGYLSGGEKQMLMIGRAILGDPKVLLVDE
                     PTEGLAPSIVAHLRNAFKELSKKAALVIVEQNLPLVCAISDKIYALKEGHIVAELADR
                     ESIKANVCERYL"
     gene            complement(3140..3937)
                     /locus_tag="VEL68_06840"
     CDS             complement(3140..3937)
                     /locus_tag="VEL68_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ35716.1"
                     /translation="MNKEPGSEIIKTENVTKRFGELTAVDRVNYLLREKEVAGIIGSN
                     GAGKTTFFNLLTGYYRPDEGTIRYRGEDITHVAPEKRVAMGMMRTFQLTSTFDNLRVI
                     DNLVLSYYRAYKKTSLLSLLLTTCKRHRTERKIAESLETFELQEVAYRQVKHLSLGEK
                     RRLEIAMAVLAEPRVLLLDEPLAGLGESEIKGVLGVLGKHVGKQTILIVEHKISQVEN
                     FLERLTVMHEGKIIADGECEECLRDPEVRRSYWQIDASTEGDEQARR"
     gene            complement(4298..5539)
                     /locus_tag="VEL68_06845"
     CDS             complement(4298..5539)
                     /locus_tag="VEL68_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35717.1"
                     /translation="MKKFLVLFSIFVFCGLLVSGDALAAEPTKIKVGILLPLTGTFAA
                     VAETQRDGALLAVDVVNKRGGLNMPWGKVQVEGTVADDEAKQDVGVRRYRYMVEEGVK
                     GVGGQTWAPLAYAINAMVLKDPMPYFPVCVMAKEAFMKGKLADSTFATAFSPWTVGYM
                     AGSAAISVLGKKKIFFLGRADSWGWDMRDGAKAAAQEKGAEMVGYDEVSLGTSDYTTV
                     LQKVRAAKPDVFIAAQFGGDAVALLKQVHQMGLNKEMTIFNSFITNVVAKGIPPEALE
                     GIYAMHYFYYDLADFEDKEVAKSAKEFTELYQAKYKIPPDSYATIAYTAYTEMFRGFE
                     AAKSLEPKKVSAALMANNGQFTTVKGPARWREDHSAVYKYAGFLVRGKGAERKGDWDL
                     FKVMGYQGGDSVLPTLKSLGY"
     gene            complement(5772..6542)
                     /locus_tag="VEL68_06850"
     CDS             complement(5772..6542)
                     /locus_tag="VEL68_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ35718.1"
                     /translation="MKRLLDRNAIVTGAAQGMGLAIAKAFYREGARVLLTDINEGAVT
                     AAAKKIDPSGTRVKWRRGDVANPEEVQAFVRELKSIWGTVDILVNNAGGALHTPHVLE
                     EIQEKDWNLVVDVNLKGTFLCCQAVIPEMAKQGKGVIVNMSALAGHWRASLAGVQYVA
                     AKAGVEGLTRQLAYDWGKLGIRVNAVAPTVTLTGDRVQGLWEAKSEEEKKKVLSAIPL
                     GRLGTPEEVASVVVFLASDESSFITGITIDVNGGRYLR"
     gene            complement(6556..>6961)
                     /locus_tag="VEL68_06855"
     CDS             complement(6556..>6961)
                     /locus_tag="VEL68_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HXZ35719.1"
                     /translation="VIAPGSINYAVWGPFPSLSGELKSRKYIIHNPNMTLVRLSPEEL
                     RSVGKLTAEKINRAKGPTRVFIPLRGFSYPDRENLPHWEPEGNRAFIDSLKAHLRPSI
                     PLRELDAHMNDPEFIDPVTDAFLSMMEEFLGR"
BASE COUNT         1535 a         2044 c         1834 g         1548 t
ORIGIN      
        1 ctaagcctaa ggccagggct tccccaatac cttcgctggc gccggttacc acagcgactt
       61 tatcgatcag gctaaattgc tccaaaggat ctttcaactt tcaaccctcc ttgttttata
      121 agagattcgc gttttgaaaa gcccttccca gaaatccctc ccgtcctccc tttttcaaag
      181 ggaggagagc ttttgacggg tcggcagatg aggattccac gttttccccc tttggaaaag
      241 ggggaggaag gaggatttta tggttcttca aaaagcgatg ccttaaaaca tatggaaagg
      301 cgaccgcctc ggtcctatcc cgcctccatc tacctcttca aaagcttgct gccgtaaaga
      361 cccgtcggtt tgatcaggag aatcagcagc atgatggcga agggcgccat ttgcgccagc
      421 ggggcgtaga taagggatcc cagggagatg acctggccga cgataaaggc agaggccagg
      481 gttcctttga tgctgcccag gccgccgatg atgaccacca tgaaaacgaa ggccagcaca
      541 tggagtccca tctgggggtc caccatgatc agcggcgcgt ggagcgcccc ggacagcccc
      601 gataacgtac cggcgaggat gaaggtaatg gtgaagagtt tgtacacgtt gatgcccaaa
      661 ccttccacat gttctacgtc ctcgatcccg gcgcgaatgg ccttgccggt gatggtccgg
      721 ttcaaaaaga gccagatgcc gaaataaacc agaaaggaga tcacgatgac catcagccgg
      781 taaacgggga tggccgtgcc cagaattatg acctgggttt cgaggggaat gggagccggt
      841 ctgggaacca acccccagat gtacttgatg gccccgacgc cggcgatcag aatcgcgaag
      901 gaggcaatca tggtgaaggt gatatcccgt tcgtaaatgc gcaggtacac gaagcgctcc
      961 acgccgaaac tgaccaggcc ccccgccacc atccccccaa acatgccgaa ccaggtgttt
     1021 cccgtggcga caaaaatgct gtagctaatg aaggcgccga cggaataata cagcaattgg
     1081 tcaaggctga tgatgcgcat caagccgaaa cagagcgaca ggccgatggc catcaggaaa
     1141 aagatgctgc cgatgctcag cccgaaaatg acgccggaaa tcaggagttg agtgtccatc
     1201 atcggctttc cccccgctca tcctggacac tcggcaagat ccccctccga gaaagggctt
     1261 ttttcaggac aggctccagc gtgctccaga tccccttttc tccggcgagc atgatgaata
     1321 ccagcaacaa accgatgaag agttcccagt tggcgataaa tccgctgatc aagtctttca
     1381 taccggtgta ggcgatcgcc ccgacgatgg ggccgtagag cgtgcccacc cctcccaaaa
     1441 tggtcaccac caccggatcg gcgcatcgcg cggggctgga tatttcaggg ctcacaaagc
     1501 ctaaataaac ggcgtagaga gagccggcca aagccgtcgt cgtgttggcg atgataaagg
     1561 ccaggtagcg aatgttgaag ttgctgtaac cgagaaactt caacttgtct tcattgattt
     1621 tggtggccag gcagcaggcg ccgtataccg agtcgtccag gtatttatag aaagcccaga
     1681 tggccatggc caccaggaaa gcgaagatga agaattcttt gggtctggtc aggtcgagca
     1741 caggggtgga ggacatgtta gtcaaaaacc acaagccgtt gtcgccgtgg gtgatgtctg
     1801 ccagaacttt ttccataagg taaaagacaa tcaccgccag ggccaggttc accagggcaa
     1861 aatagtcgct ccgcaaccgg acgaaaaagg gaccgacgaa ggccgctaca gctgccccga
     1921 tcagaagacc gaggaggatt ccaatgtagg ggttttggcc gaagtaaaag aggtaaaaag
     1981 cagtggcata ggcgcccacc gccagatagg ccggctggcc gaaggaaata aaaccgaccc
     2041 gccccagaag gaagttgcag cccatggtat aaatagaaaa aataatgatc tcgattgcga
     2101 agggcagggg ggagaatact ctcaacaagg ccagcagcga aaaggccaac aagatgtccc
     2161 accaccacct gaatatgttt gtcatgggaa tttccttttt cagtctccgc tttacggaag
     2221 aacggatcct tcagccccga tccgaggcgc cgggtgcggc ctgggcggct ttcccctcca
     2281 gcttcaacct acttgaaacc tagcaggttg aggaaaaacc ttttccaggc ctgcagtgtc
     2341 attcccgcgg aggcgggaat ccagtctttt caatgccttc cagactcccg ctttcgcggg
     2401 agtgacggct cacaggagtt tttccgcatc ctgctagagg taccgttcgc acacgttggc
     2461 tttgatggat tcccggtcgg ccagttcggc gacgatgtgc ccctctttga gggcgtagat
     2521 cttgtcggat atggcgcaca ccaggggcag gttctgttcc acgatcacca gcgccgcctt
     2581 tttgctcagt tctttgaaag cgtttctcag atgggcgaca atgctggggg ccaatccctc
     2641 ggtgggctcg tcgacgagca acaccttcgg gtcgcccagg atggctctgc cgatcatcag
     2701 catctgcttt tccccgccgc tcaggtatcc acccttgcgg tccatgaggc ttttcaacct
     2761 ggggaaatgg tccgtgacct gatcccagtt gtaatccttg gtggcatagc tgcccagctc
     2821 caggttttcc ctgacggtca aatcggaaaa gaccttttta tcttggggaa tgtatttgag
     2881 ccccatcctg gcgatgtcgt aggacatgag cccgaccagg ttggtatcat tgaagatgat
     2941 cgatccggag gtcggcttta agaatccagc aatcgtcttg agcaacgtgg ttttgccggc
     3001 accgtttcgg cccacgcagg tcaccacctg ccccggcttc acccggaggc cggcatcgaa
     3061 cagaaccttc gactctccgt aagaaacatt caggccttga accgccagga tttcgccgtc
     3121 attcatgatc gtggactccc tatctacggg cctgttcgtc tccctcggtg ctggcgtcga
     3181 tctgccagta gctccggcgc acttccggat cccggagaca ttcctcacac tcgccgtcgg
     3241 cgatgatttt gccctcatgc ataacggtga gcctctccag gaaattttcc acctgggaaa
     3301 tcttatgttc cacaatcagg atcgtctgct tgcccacgtg cttgcccagc acccccaaga
     3361 cccctttgat ttccgactcc cccagcccgg ccagaggctc gtccaacaac aataccctgg
     3421 gttcggccag gacggccatg gcgatctcca gccgtctctt ctctcccagg ctgaggtgct
     3481 tcacctgtct gtaggccact tcctgaagct caaaggtttc cagggattcg gcgatctttc
     3541 gttccgtgcg gtgccgcttg caggtcgtaa ggagcaggga aaggagggac gtttttttat
     3601 aggcccggta gtaggacagg accaggttgt cgatcacccg gagattgtca aaggtggagg
     3661 tcagctgaaa ggtccgcatc atccccatgg ccacccgctt ttccggggcc acatgggtaa
     3721 tgtcctcccc ccggtatcgg atcgtcccct catcgggccg gtagtatccg gtcaagaggt
     3781 tgaaaaatgt ggtcttcccg gccccgttgg acccgatgat tcccgccact tctttttccc
     3841 gcagtaaata attcacgcgg tccacggcgg tcaattcgcc gaaccgcttg gtgacatttt
     3901 cggtcttgat gatttccgaa cctggctcct tattcacggc ggcccttttt ttctggtccg
     3961 acccggtgga aggaggcgac ctccaccggg tcggatggtg ctttgcagat tatcctcaaa
     4021 tagattgtga tcgctaaatc ttccctcacc cccaccctac ccccaccctc gccctccccc
     4081 ctcgaggggg agggaattcc tttactattt caatgtggtt atcctctccc ctcgcgggag
     4141 agggtagggt gaggtgggca tttaagggta cgttttaatg cagacgcatt ttattttttg
     4201 acattatgta gggcctgatc cccgatcagg cctaaagcat cggtgcgttc ggggaacgca
     4261 ccctacattt agggcaaatt catttaatgc atttgcatta ataacccaga gacttcaggg
     4321 tcggcaacac cgaatcgccg ccctggtagc ccatgacttt gaaaaggtcc caatcgcctt
     4381 tcctctcggc cccttttccc ctgaccaaga agcccgcgta cttgtacacc gccgaatgat
     4441 cctcgcgcca ccgggcagga cctttgaccg tagtaaattg gccgttgttg gccatcagcg
     4501 ccgcggacac tttttttggc tcaagggact tggccgcctc gaagccgcgg aacatttccg
     4561 tgtaggcagt ataggcaatc gttgcataag aatcgggagg gattttgtat ttcgcctggt
     4621 acagctcggt gaactccttg gcgctcttgg ccacttcctt gtcctcaaag tcagccaggt
     4681 catagtaaaa atagtgcatc gcatagatgc cctccagggc ctcgggagga atgcccttgg
     4741 cgaccacgtt ggtgatgaaa gaattgaaaa tggtcatttc cttgttgagg cccatctggt
     4801 ggacctgttt taaaagggcc accgcatcgc caccaaattg agcggcgata aaaacatccg
     4861 gctttgccgc cctgactttc tgcaaaacgg ttgtgtaatc actggtcccc agggatacct
     4921 cgtcatagcc aaccatttca gcaccttttt cctgggctgc cgctttggcc ccatcccgca
     4981 tgtcccagcc ccagctgtcg gcgcggccta agaagaaaat ttttttctta cccagcacgc
     5041 tgatggccgc ggacccggcc atgtatccca cggtccaggg actgaatgcg gtggcaaagg
     5101 ttgaatcagc cagcttgcct ttcatgaaag cctctttggc cattacgcaa acggggaaat
     5161 aaggcattgg gtcttttaaa accatggcat tgatggcgta agcgagcggc gcccaggtct
     5221 gaccgcccac gcctttaacc ccttcttcaa ccatgtaccg gtagcgccgg acacccacat
     5281 cttgcttggc ttcatcatcg gcgacagttc cctccacctg cacctttccc cagggcatat
     5341 tcagcccgcc cctcttgttg accacatcca cggccaacag ggcgccgtcc ctctgggtct
     5401 ctgccaccgc ggcaaaagtg ccggtcaggg gaagcagaat cccgaccttt attttcgtgg
     5461 gttcagccgc caaggcgtcg ccggaaacca aaagaccgca gaatacgaag atagagaata
     5521 gaaccagaaa ctttttcatt tgcccctcct tctgattgaa tatcctgagt ttcatatcct
     5581 ccgcgagcgg ccttcatctt taaatttcgg taacttcaca tcacctcccc gatgggtcta
     5641 atggcggcgg gtcgccatga accctgcaca atgcccaacc cttgggaccc gctttttgag
     5701 tagggtgagt atacttggaa ggacaatgtg cgtcaaggaa tttaaactcc agaatgcgaa
     5761 ggcagaattc cctaccgaag ataccgccct ccattcacat caatggtaat cccggtgatg
     5821 aaactggatt catcggatgc tagaaaaaca accacggaag cgacttcctc gggagtcccc
     5881 aaccggccaa gggggatagc ggaaagaacc ttctttttct cctcttcgct tttggcctcc
     5941 cagagccctt gaacccggtc cccggtcagc gtcaccgtgg gcgcgacggc attcacccgg
     6001 attcccaact tcccccagtc ataggccagc tggcgggtca gcccctccac ccctgccttg
     6061 gcggcgacgt attgaacccc ggccagcgaa gccctccagt gccctgcaag ggcagacata
     6121 ttgacgatca cccctttccc ctgcttagcc atctcgggaa tcaccgcctg gcagcagaga
     6181 aaagtccctt tcaggtttac gtcaaccacg agattccagt ctttttcttg aatctcctcc
     6241 agcacgtggg gcgtatgaag ggctccaccg gcattgttca ccaggatgtc gaccgtaccc
     6301 cagatcgatt tcagctccct gacgaaggcc tgcacttcct cagggttggc cacatctccc
     6361 cttctccatt tcacgcgagt cccgctggga tcgatcttct ttgcggcggc ggtcacagcg
     6421 ccctcgttga tgtcagtgag cagcacccgg gctccttccc ggtagaaggc tttggcgatc
     6481 gccaggccca tgccctgggc tgcgccggtc acgatggcat tccggtccaa gagacgcttc
     6541 aaatttttac ctccttcatc gccctagaaa ttcctccatc atggataaaa aggcatccgt
     6601 caccgggtcg atgaactccg ggtcgttcat gtgagcgtcg agctccctca gcgggatcga
     6661 ggggcgtaaa tgggccttca gggaatcgat gaaggcccgg tttccctcgg gctcccagtg
     6721 gggcaggttc tcccggtcgg ggtaggagaa tcccctcagg gggatgaaca cccgggtcgg
     6781 tcctttggcc cggttgatct tctccgcggt gagttttcct acgctgcgca gttcctccgg
     6841 gctcagccgc accagggtca tgttgggatt atgaataata tattttcggg acttcagttc
     6901 cccggacagg ctaggaaaag ggccccacac cgcataattg atggaccccg gagcgatcac
     6961 c
//
LOCUS       DASPRR010000175         8477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_691295, whole genome shotgun sequence.
ACCESSION   DASPRR010000175 DASPRR010000000
VERSION     DASPRR010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691295"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VEL68_06860"
     CDS             complement(<1..398)
                     /locus_tag="VEL68_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35720.1"
                     /translation="MGRHDDDDDREKLSWREIDKMRDHSRHVSREPKSFQERSLRSDW
                     AKKQHLREAEKFFQGQKGTEEYKKAYTALHDKYGTPRFEEAAKKFVQEFGPPDDWGTL
                     ILLLDAKDPALAKAALGPLKGLYQKRSPIE"
     gene            complement(693..989)
                     /locus_tag="VEL68_06865"
     CDS             complement(693..989)
                     /locus_tag="VEL68_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35721.1"
                     /translation="MSTANTMEKRKEKINKAVSCQRCGGRMIFEKFYDVNNIFFGWHC
                     VMCGEILDPVILLHRLSQDADLQIPEGEEQVMQLLKKYFQSGTLPARKGADIKV"
     gene            1597..1941
                     /locus_tag="VEL68_06870"
     CDS             1597..1941
                     /locus_tag="VEL68_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HXZ35722.1"
                     /translation="MSERRPWGSYTILEEREAYKVKRIEVLPAKRLSYQRHQKRDEHW
                     VIVEGEALVTVEGKDIPLSPGGSVDIPRMAAHRILNPGKSLLIFIEVQKGDYFGEDDI
                     IRLEDDFGRFAG"
     gene            2090..3292
                     /locus_tag="VEL68_06875"
     CDS             2090..3292
                     /locus_tag="VEL68_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35723.1"
                     /translation="MESEEKFILGANYWPAEKGLYWWREFDLSAGKRDFSLAAEYGLE
                     LMKVFLLWEDFQPEINRISVRTLENLVRVADLAQDRRIKILPVFFCGHMRGVNWLPSW
                     MVERGKGEGRYPVFSMGKIRKGRVRNMYSDREVRKAQKLLIHETTDALQDHPAVWGWD
                     LGNQPSKLIYPPSRDSARAWFEEMVTELRRWNSSLPITWSMDQSDLEEGRIPGPKELA
                     PYCDFMSLQMDPGQAKWADGPLDEKVPLYLCLLAQWLGGKGALVGDLGVPTEPLFPYL
                     TEGDQKKLGGVGLVKEGDAETFSEKSLDLLKNHGIKGALARSFSDYDSSLWDRPPLDE
                     KVTERFCGLFRQDGSAKPAARMVRDFPRGKKARDLLWDWVDIRPEEYYEDPLGQLTRL
                     YRRFKDRL"
     gene            complement(3429..3504)
                     /locus_tag="VEL68_06880"
     tRNA            complement(3429..3504)
                     /locus_tag="VEL68_06880"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3468..3470),aa:Glu,seq:ttc)
     gene            3582..4118
                     /locus_tag="VEL68_06885"
     CDS             3582..4118
                     /locus_tag="VEL68_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HXZ35724.1"
                     /translation="MNRILRFLAIAFFLTLVGLVAGPGEWSAEAEFYKYVDKKGNVHF
                     TDRFESIPQEYRNQIKEYKEMKEPEALPAQEKEESKDPAAAERDRQKKEAEQKMKEAE
                     AKAAQEKSLQEEKLKIRQEKEKQIEELQEQITERQKEQRSLRTNWMVYDRIRLNQLNE
                     EIGGLEKQIGDLQKELSE"
     gene            4472..5623
                     /locus_tag="VEL68_06890"
     CDS             4472..5623
                     /locus_tag="VEL68_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35725.1"
                     /translation="MAVQFFREEHEIFRKSLRSFLEKEVKPHQDEWRKNRQVPREVWL
                     KMGEQGFIGYWLDEKYGGVGADFVYSVVLIEELNRAGVRGFSAGLGVHNDIVMPYIEM
                     LGTEEQKMRWLPKCCTGECILAIGMTEPGAGSDLQAIRTTAVRDGDDYVINGQKTFIT
                     NGYYCDLIVLAVKTDLKAQPPYKGVSLIVVENGTPGFVKSRKLEKMGLHSSDTAELFF
                     EDCRVPASNLLGREGDGFVPMMRNLQQERLVSALGAILSAERMLETTIEYCKTRTVFG
                     KPISRHQHNTFKIVEMATEVEMAKDFTYHLVEDHLEKKDITKKVSMCKWYNAELSNRV
                     AYQCVQLHGGYGYMDEYQICRDALDVRMGSIAAGTTEVMKMIIARLMGL"
     gene            complement(5733..6437)
                     /locus_tag="VEL68_06895"
     CDS             complement(5733..6437)
                     /locus_tag="VEL68_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35726.1"
                     /translation="MSLLAIESVHSGYGKMTVLEGVNLKVERGEFVVVIGPNGAGKST
                     LLKTIFGFADLHRGKITYEAREISGLKPEEVSQRGVAYVPQDNNIFPSLTVLENLEMS
                     AILYADPPRARLERVFSRFPRLQERRYQSAGTLSGGERQMLAIGCGLMIEPRLMLLDE
                     ITGGLAPLLVAALFEAVHGVHQEGTSLLMVEQNAQKALNVGQRGYILEGGKVKLEGQA
                     DELLKNEEVIKHFLGV"
     gene            complement(6439..7200)
                     /locus_tag="VEL68_06900"
     CDS             complement(6439..7200)
                     /locus_tag="VEL68_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35727.1"
                     /translation="MTILLEAREVVKDFGGLRAVDHCSLSVERGSICGLIGPNGAGKT
                     TLFDLLTGFYKPNSGTVFFKEQRIDGMEPHLICLQGLVRTFQIARELKNMTLLENMLL
                     APKGQYGEILGYAIARPPKVLARERENLEKALGILEIVGLEHLKDEVAINLSGGQRKM
                     LEVARALMTEPEMLLLDEPTAGATFEETRKLMDYIEKLRKEHGLTFLIVEHKMEVIMN
                     LCDKIFVMNNGRKLTEGTPQEVQRNPQVIEAYLGA"
     gene            complement(7197..8147)
                     /locus_tag="VEL68_06905"
     CDS             complement(7197..8147)
                     /locus_tag="VEL68_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35728.1"
                     /translation="MEGFLTFIISIATIVAIYSILTLALNIRWGFTGLLDFGIAGFFA
                     IGAYTSAILTSPPSEELVFGVRHSLGFGVPFAGGVLAAAAAAALVAYLIGIPTLRLRG
                     DYLAIMTIGLSEIFRYMAVNEKWLTRGSQGIRNLPRPFSGYSNLLDSLFYFLLVVVIL
                     FLLYLISQRMYNSPLGRVLKGIREDETVTEALGKRIFHFRMLAFISGAAFAGVAGSLW
                     AHYSGSIMPHEFAPDITFLIWVSLTIGGSGNNRGAILGTLVLIGLFQQGTRFLPTIEG
                     YTYLIPGLRLIAIGFMLILCLRFRPQGLLPEKAVRVEKGL"
     gene            complement(8151..>8477)
                     /locus_tag="VEL68_06910"
     CDS             complement(8151..>8477)
                     /locus_tag="VEL68_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35729.1"
                     /translation="ARIRGIRTEKVVVWTWLLSCGLAGLAGVMLGLMGAVDNHMGIEM
                     LLVIFASVIMGGIGNPYGAVAGAMIIGIAMEVSSAFGLSEYKSALAYLLMAIILLLKP
                     KGLFAR"
BASE COUNT         2000 a         2305 c         2352 g         1820 t
ORIGIN      
        1 tgctcgatgg gacttcgctt ttggtagagc cccttcaagg gacccagggc tgccttggcc
       61 agggccgggt ccttggcatc gaggagcaaa atgagggtgc cccagtcgtc tggggggccg
      121 aattcctgga cgaacttctt cgctgcttct tcaaacctgg gcgttccgta tttgtcgtgg
      181 agagcggtgt aggctttctt gtattcctcg gttcccttct gcccctggaa aaatttctcc
      241 gcttcccgca agtgctgctt cttggcccag tccgaacgca ggctgcgttc ctggaagctc
      301 ttcggctccc ggctcacatg gcggctgtga tcccgcattt tatcgatctc ccgccagctc
      361 aatttttccc ggtcgtcgtc atcgtcgtgt cggcccatag gttctctcct tccggggctc
      421 atttaatcaa aaacgaaaaa tttcttcaag ccaaatcaaa gatagaattc gggagatgtc
      481 atggcgaatg gccaaaaggg atggcggagg ctaagaagag gtgagcatgc aggtgggaga
      541 atggggaaga ggatttccag gaggaaagag acgaaagaga aagaaggaat aaatgggtgg
      601 agaaaacaaa gaggggcgag ccttttgctc acccctcttt aggttcatgc gacccttccc
      661 ctgccggacg gcataaacgg gtaaacttcg gatcagacct ttatgtcggc acccttcctg
      721 gctggcagtg tgccggattg aaaatacttc ttcagaagct gcatgacctg ctcttcccct
      781 tccgggattt gaaggtcggc atcctggctt aggcggtgaa gaaggatgac cgggtccaaa
      841 atctctccgc acatgacaca gtgccacccg aagaaaatat tgttcacgtc gtagaacttt
      901 tcaaaaatca tccttccccc acaacgctgg cagctgaccg ccttgttgat cttttctttt
      961 cgcttttcca tggtattggc agttgacatg atatttccta cacgacctcc ttgtttttgt
     1021 tcggacttcg caagcgattc tctttggaac acggtgaaac tctccccccc ccacgccttc
     1081 ctgcttgtac cagattatcg aggacagcag aggaaataaa tagggtgaat cccgtatttt
     1141 ttggtttaaa atcctgtatt atgactgggg atgaaggcgg ggattcagag gctccctaaa
     1201 ggcgcggaat tcttatctcc cagactttcc cctatggttg cgacttccac ctgccgcgac
     1261 ttccatccgt ttcccacctt gcagccccgt ttttgggagg agcgagggaa ctgacgcgct
     1321 ctatcccaac ccttctcgtg ccaattctta tggggaagtc acttcagcga agaccctgct
     1381 ttttcgggaa ttgaatcctt ttcttcccga aaatttaata gtatatatta ttagcctttc
     1441 gaagccatgt caagaaaaaa atgcatcgag agcgccccct ttttgtttgc ccagaggaca
     1501 tgtatttttt tagaatgaaa ccgtcgcccc tcggaagttt tcggaatttg cagaccgcgg
     1561 ggaagccgcc cccgtgcaag aatggaagga atgaccatga gcgaacggcg tccatgggga
     1621 agctatacga ttctggaaga aagggaagcc tacaaggtca agaggataga ggtcctaccc
     1681 gctaaaagat tgagctatca gaggcatcaa aaaagggacg aacactgggt gatcgtggaa
     1741 ggggaagcgc tcgttacggt ggaggggaag gatattcctc tctccccggg aggctctgta
     1801 gacattcccc gcatggccgc ccaccgtatc ctaaaccccg ggaaaagcct gctcatattc
     1861 attgaagttc aaaaggggga ctatttcgga gaagacgaca tcatccgtct ggaggatgat
     1921 tttggaagat ttgcgggcta gctgctccca tcctcaccct cggcttctcg gcaagtaaaa
     1981 ttttaaaccg ctcctttcca gactttcata ggtccgaatt ctttatgctg ctttgcgttc
     2041 tggagggtcc gtttacattg acccggcaga aaggatagct tcctaaacga tggagagcga
     2101 agaaaagttc atcctggggg ccaactactg gcccgcggaa aaaggccttt actggtggag
     2161 ggagtttgat ctctccgctg gcaaaagaga cttttcgctg gccgccgaat atggcctcga
     2221 actgatgaaa gtctttctgc tctgggaaga ttttcagccc gaaatcaacc gcatctcggt
     2281 aagaaccctg gaaaacctgg ttcgcgtggc cgacctggcc caggaccgcc ggataaagat
     2341 cctgcctgtc ttcttctgcg ggcatatgag gggggtgaac tggcttcctt cctggatggt
     2401 ggagagagga aaaggggagg gaaggtatcc ggtattctcc atgggcaaga ttcggaaggg
     2461 gagggttcgg aacatgtatt cggaccggga ggtgcggaag gcccagaaac tcctgatcca
     2521 cgaaaccacc gatgccctgc aggatcatcc cgctgtatgg gggtgggact tgggcaatca
     2581 accctccaaa ctgatctacc ccccctccag agactcggcc agagcctggt ttgaagaaat
     2641 ggtgaccgag ctgaggcgtt ggaactcctc ccttccgata acctggagca tggaccagtc
     2701 tgacctggaa gagggacgga ttccaggccc caaagaactg gctccctatt gtgacttcat
     2761 gtcccttcag atggaccccg gtcaagccaa atgggctgac gggcccttag atgaaaaagt
     2821 ccctctatac ctttgcctct tggcccaatg gctggggggg aagggtgcgt tggtggggga
     2881 cctgggagtc cccacggagc cccttttccc ataccttacc gaaggggacc agaaaaaact
     2941 tggaggagtt gggttggtca aagaaggcga tgccgaaacc ttctctgaaa aaagcctaga
     3001 ccttttgaag aaccacggga tcaagggcgc gctcgcccgc tctttttcgg attatgattc
     3061 ttctttatgg gaccgaccgc ccctggatga gaaggtgacg gagcgctttt gcggcctctt
     3121 ccgacaggat ggatcggcta aaccggcggc gcggatggtt cgggactttc cccggggaaa
     3181 aaaagcccgg gaccttctat gggactgggt ggatatccgc ccggaagaat actacgaaga
     3241 ccccctcggc caattgactc gcttgtatcg aaggtttaaa gaccggcttt aatgatcctt
     3301 tgctgccatt gcccaaaatg atggtgctgc cgcccgtaag aaaagcctaa atttgaatcc
     3361 aaatggacac cctgtgcgca ccgaaaaaga aaaagggtta gcttttcgtg ctaacccttt
     3421 attttctttg gcgtccccaa ccggattcga accggtgttg ccggcgtgaa aggccggtgt
     3481 cctaggcctc tagacgatgg ggacccaaaa tgttttttag ggttttaaag ttactataaa
     3541 aacatgtcga tgtaaagaaa aaagcatggt aaattaatat tatgaatcga attctcaggt
     3601 ttctcgccat cgcatttttt ttaaccctgg ttggcctcgt ggccggtccg ggagaatggt
     3661 ccgctgaagc tgaattctac aaatatgtag ataaaaaagg gaatgttcac ttcaccgatc
     3721 gctttgaatc gattccccaa gaatatcgga accagataaa agaatacaag gagatgaaag
     3781 agcctgaggc tcttcctgcc caggaaaaag aagagtccaa agaccctgcc gctgcagagc
     3841 gggaccgtca gaagaaagaa gccgagcaga aaatgaagga ggccgaagcc aaggctgccc
     3901 aagaaaagtc cttgcaggag gaaaaactta agattcgtca agaaaaggaa aagcaaatcg
     3961 aagagctcca ggaacagatc accgaaaggc aaaaagaaca aagaagcctc agaaccaatt
     4021 ggatggtcta tgaccggatc aggctgaacc agttgaacga ggaaatcggg ggcttggaaa
     4081 aacagatcgg tgatctccaa aaagaactgt ccgaatagcc aactatcccc ttccgttatc
     4141 gacctgccga tttctatcat tcctttccac catgtacgca atcaatgttt gtggtccacc
     4201 tttcgctctt atctaaaatc cgctgcctgt aatctgacat ccctcagttg tgcccaacgt
     4261 cataagctat caaggccaaa gccctcaggt acaagtacag gtcctaactg ccatgttatt
     4321 caactcttgc agggttgaac aagccgccac cgctgacgaa tcatcctgac cattccttaa
     4381 cccccaaatg aaaaaaagga ttgagtattt ttccactttt ttctatatat gaggccagga
     4441 aatataattc tgttgaaagg gggggatgaa gatggctgtc caattcttcc gtgaagaaca
     4501 cgagatcttc cgcaagtccc tgcgcagttt tctggagaag gaggtcaagc cccaccagga
     4561 cgagtggcgc aaaaaccgcc aggttcccag ggaggtctgg ctcaagatgg gggaacaggg
     4621 ttttatcggc tattggctgg acgagaaata tggtggcgtg ggagcggatt tcgtctattc
     4681 cgtggtcctg atcgaagagc tgaatcgcgc cggggtccgg ggattctccg ccgggctcgg
     4741 ggtgcataac gacatcgtta tgccttacat cgagatgctg gggaccgagg aacagaaaat
     4801 gcgttggctc ccgaagtgct gcaccggcga gtgtatcctg gccattggaa tgaccgaacc
     4861 cggagcgggt tcggacctcc aggccatccg caccacggcg gtccgggacg gcgatgacta
     4921 cgtcatcaac ggccagaaga ccttcatcac caacggttat tactgcgacc tgatcgtcct
     4981 ggcggtgaaa accgacctca aagcccagcc accttacaaa ggggtctccc tgatcgtggt
     5041 cgagaacggg acccccggct ttgtcaaaag ccgcaagctg gaaaaaatgg gcctccattc
     5101 ctcggacacg gccgagctct tcttcgagga ttgccgggtc ccggcctcca accttctggg
     5161 ccgggaaggg gatggattcg tccccatgat gcgcaacctc cagcaggaac ggctggtctc
     5221 ggccctgggc gcaattctga gcgctgaacg gatgctggaa accaccatcg aatactgcaa
     5281 gacccgcacc gttttcggga agccgatttc ccgccaccag cacaatactt tcaagatcgt
     5341 ggaaatggcc acggaggtgg agatggccaa ggatttcacc taccacctgg tggaggacca
     5401 cctcgagaag aaagacatta ccaagaaggt ctccatgtgc aagtggtaca acgcggagct
     5461 atccaaccga gtggcctatc aatgcgtcca gctccacggg gggtacgggt acatggatga
     5521 gtaccagatc tgccgggacg ccctggacgt acgcatgggg tccatcgctg cgggcaccac
     5581 cgaggtgatg aaaatgatca tcgcccggct gatggggctg taataattgc agatggcgga
     5641 atgcggattt cgggtttgag attgtggaag agatttggac gaaggccctg ggtctattca
     5701 atccgcaatc cgcaatcgaa aatccgaaat tgttagactc ccagaaagtg cttgatcact
     5761 tcctcattct tgagaagctc gtcggcctgt ccctcaagtt ttaccttccc tccctccagg
     5821 atgtatcccc tctgcccgac gttcagggct ttctgcgcgt tctgttccac catcaagagg
     5881 gaagtccctt cctgatgaac cccatgaacg gcttcgaaga gggccgccac caggaggggc
     5941 gccagcccgc cggtgatctc gtcaaggagc atgagccgcg gctcgatcat cagcccgcat
     6001 cctatggcca gcatctgcct ttccccgccg cttaaggtcc cggcgctctg atagcgcctt
     6061 tcctggagcc gcggaaacct ggaaaaaacc ctttccaggc gggccctggg ggggtctgcg
     6121 taaaggatgg cactcatctc caagttctcc agcacggtga gggaagggaa gatgttattg
     6181 tcctggggga cgtaagccac gccccgctgg gagacctcct cgggcttgag gccgctgatc
     6241 tcccgcgctt cgtaggtgat cttgcctcgg tggaggtcgg cgaagccgaa gatggtcttc
     6301 aggagggtag atttgccggc cccgttcggc ccgatgacca cgacgaactc cccccgctcc
     6361 accttcaggt tgacaccctc caggacggtc atcttgccgt aaccggaatg gaccgattcg
     6421 atggccagca ggctcatttc aagctcccag ataggcttcg atgacctgag ggttcctctg
     6481 aacctcctgg ggagttcctt cagtcagctt ccggccattg ttcatgacga aaatcttgtc
     6541 gcagaggttc atgatcactt ccatcttatg ttccacgatc aggaaggtga ggccgtgctc
     6601 cttgcgcagc ttttcgatgt aatccatgag tttacgggtt tcctcaaaag tcgccccggc
     6661 agtcggttcg tcgaggagga gcatttccgg ctcggtcatc agggcccggg ccacctccag
     6721 catcttcctc tgccccccgg agaggttgat ggccacctca tctttcaagt gctccagccc
     6781 cacgatctcc aggatcccca gcgccttttc caggttttcc ctctctcggg ccaggacctt
     6841 gggcggccgg gcgatggcgt agccgagaat ttccccgtac tgccctttgg gggccaggag
     6901 catattctcc aaaagggtca tgttcttaag ctcccgggca atctgaaagg tccggaccaa
     6961 cccctggagg cagatcaggt ggggctccat cccgtcgatt ctctgctcct tgaaaaaaac
     7021 cgttcccgaa ttaggcttgt aaaacccggt gagcaggtcg aagagggtcg tcttgccagc
     7081 cccattgggg ccgataagcc cgcagataga ccctctctcc acggagaggc tacagtggtc
     7141 caccgccctc aacccgccga agtccttgac gacttccctg gcttccagaa ggatggtcat
     7201 aggcccttct ccacccggac cgctttctcg ggcaggagcc cctgaggccg gaagcgcagg
     7261 cagaggatca acataaagcc gatggcaatc aatcgcagcc ccgggatcag gtaggtatag
     7321 ccttcgattg tgggcaggaa acgggtcccc tgctggaaga gtccgatcag gaccagagtc
     7381 ccgaggatgg cccccctgtt gttcccgctt cccccgatgg tcagggagac ccagatcagg
     7441 aaggtgatgt ccggggcgaa ctcatggggc atgatggagc cgctgtaatg ggcccagagg
     7501 ctcccggcca cacccgcaaa ggcagccccg gagatgaagg ccagcatgcg gaaatgaaag
     7561 attcgcttcc ccagcgcttc ggtcaccgtc tcgtcctcgc ggatcccctt gagaaccctt
     7621 cccaggggag agttgtacat gcgctgggag atcaggtaga gcaagaaaag aatgaccacg
     7681 accaggagga aatagaagag actgtccagg aggttggagt agccggagaa gggccgggga
     7741 aggttgcgga tcccctgaga gccccgggtg agccactttt cgttgaccgc catgtagcgg
     7801 aaaatctcgg acaatccgat ggtcatgatg gccagatagt ctccccggag gcgcagggtg
     7861 gggataccaa tgaggtaggc gaccaaggct gccgcagccg ccgcagccag gacccctccg
     7921 gcaaagggaa cgccgaagcc tagactatgg cgaacgccga agaccagctc ttccgaaggg
     7981 ggagaggtga ggatcgccga ggtgtaggcc ccgatggcaa agaagccggc gattccgaag
     8041 tcaagcaggc cggtgaagcc ccagcggatg ttcagggcca gggtcaggat ggagtagatg
     8101 gccacgatcg tggcaatgga gatgatgaaa gtaaggaacc cttccatcgc ttacctcgca
     8161 aagagcccct tgggcttgag gagcagaatg atagccatta acagataggc cagggccgac
     8221 ttgtactcgg aaaggccgaa ggccgaggag acttccatgg caatgccgat gatcatggcc
     8281 ccggccaccg ccccgtaggg atttccgatc cctcccatga tcaccgaggc gaagatcacc
     8341 aggagcatct cgatccccat atggttgtcc accgcgccca tgaggcctag catcaccccg
     8401 gccaggcccg ccaagccgca gctaagaagc caggtccaaa cgaccacctt ttccgtgcgg
     8461 atcccccgaa tccgcgc
//
LOCUS       DASPRR010000176         7732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_530400, whole genome shotgun sequence.
ACCESSION   DASPRR010000176 DASPRR010000000
VERSION     DASPRR010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530400"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2684)
                     /gene="fdhF"
                     /locus_tag="VEL68_06915"
                     /pseudo
     CDS             complement(1..2684)
                     /gene="fdhF"
                     /locus_tag="VEL68_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043674.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(2711..5836)
                     /locus_tag="VEL68_06920"
     CDS             complement(2711..5836)
                     /locus_tag="VEL68_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ35730.1"
                     /translation="MKETLRSAKDLTSFRESILRGWDPQKTCVTICGGTGCRAWGGEE
                     LRIAFIQEIQKQSLGEKVDVMRTGCHGFCERGPVVVILPKEIFYQQVVVDDVPEIVAQ
                     TLAKGQVVQRLLYTDPATGQVITYDHEVPFYRRQKRMVLSDNGRIDPTEIRDYIARGG
                     YSALAKVLSSMTPDQVVEEVERSGLRGRGGAGFPTGRKWRFTSQAPGKGKYLVCNADE
                     GDPGAFMDRSVLEGNPHLVLEGMLIAGYAIGAQSGHVYVRAEYPLAVQHLTLAIEQAE
                     ELGLLGDNVLGSSFGFHLKIKEGAGAFVCGEETALLASIEGKRGMPRARPPFPAIAGL
                     WGKPTNINNVETYANIRSIILGGAEAYAAVGTAGSKGTKIFSLTGKINNTGLVEVPMG
                     TTLRQVIYDIGGGIPRGRRFKAAQMGGPSGGCLPPQYLDLPIDYESLTQAGSMMGSGG
                     MIVLDEKTCMVDIARFFLSFTQDESCGKCVPCRIGTKRMLEILTRITRGEGQEGDIEL
                     LLEMGPIIKDASLCGLGQTCPNPVLSTIHHFRQEYEAHIRGKFCPAGACEALVFSPCE
                     NTCPVRCDAVGYAALIGEGRHEEALSLIRLNMPLAGICGRVCDHPCENMCKRGEIDEP
                     LAIASLKRFASDWERKQGKLAPPAFLEKMKEDRVAVVGAGPAGLNAAYQLGRRGYRVT
                     VFEALPVAGGMLAVGIPDYRLPREIMEHDLRFICQHNVEIQTGKALGRDFSLEELLRG
                     GYKAIFLALGAHRNQRLNIPGEETPGVLPGIDFLRRVNLGEKVELGGKVAVVGGGNVA
                     FDAARTALRLGAREVSIVYRRTREEMPANDEEIQEAEHERIRFLYLVAPTRIVARNGK
                     VEGLECQRMELGDFDATGRRRPVPVKGSEFILEVDWVIPAIGYIPDLSCLPQGDGFQI
                     TKANTLSVDPVTMATHIPGVFAGGDVVTGPSTVVEAMAGGYQASVSIDRFLKGQDLRQ
                     NRVYQPLRRAEVPKVEDGEEEKEAAKPRAQMPANPVDRRVCTFEEVNLGFDEETAVRE
                     AQRCLRCDLKG"
     gene            complement(5833..6312)
                     /gene="nuoE"
                     /locus_tag="VEL68_06925"
     CDS             complement(5833..6312)
                     /gene="nuoE"
                     /locus_tag="VEL68_06925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HXZ35731.1"
                     /translation="MPEEIDFSKLDPILEKYRGQEGALIPVLQEAQALYGYLPEGVLK
                     AISQRLKISISRVYGVVTFYSQFYTTKRGRHTVRVCRGTACHVRGGKGILKAVQQILG
                     VGENETTPDYEFTFETVACLGACALSPVLLVDKKYYGKLTPAKVDRVLKEYSRGGGS"
     gene            complement(6528..7463)
                     /locus_tag="VEL68_06930"
     CDS             complement(6528..7463)
                     /locus_tag="VEL68_06930"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXZ35732.1"
                     /translation="MRIAAVGTGALGSLYAGYLSRSGEEVYAVDIKEDIVSAIRSSGI
                     RIVEPEGKEVSIPVKNATLKPEEVGKVDLVIFLPKSRQTREAAQSARCLFGPETVGLT
                     VQNGLGNPEAIEAVVGEGRILAGVTLNASTYLGPGRMLYAGRGETVIGEMRGGPSPRA
                     EKIAAAFNKAGLSTHVSSEVWNDVWGKLLVNAGVNPLTAITRLANGVLVDQAAAREIM
                     RGLVEEGKRVAQAKGIRLPYTDAVKKVEDACIATKPNYSSMLQDVLAQRETEVDFING
                     AIVREGERLGIDTPINRTITNLVRAIEKTYDRRVK"
BASE COUNT         1397 a         2502 c         2176 g         1657 t
ORIGIN      
        1 accttcttga tattcaccgc gcagaccttg agcccgggga tcttggccac cgggtccagg
       61 gcgtccaggg tgagcaggtt caccgccgct tccttgaagt ggaaggtcat gaagaccact
      121 cctggaggga gcgcttccga gatcttggct ttggcctgca cctttcccct ccgcgaactc
      181 acctcgacca tcccgtcgcc ggaatgaatt cccagccggg aggcatccgc cgggctgatc
      241 tccacggtcc cttccggata gatctcggac agtcccttgg acttgcggct gatcaccgtg
      301 tggtaatggt aaaggacccg tcccgtggtc agggtgaagg gatactctgc gtccggcagc
      361 tcgggcggcg ggacgtaggg ggtcacatga aacttcccct tcccgcgggt gaacatccct
      421 tgatggagga tcggagttcc gggatggacc ggagtggggc agggccattg cagccccgat
      481 ccttccagcc gctcataccg gatccccccg taggagggag tgagggaggt gacctcctcc
      541 atgatcttct ccggggagtt gtacttcatg gggtacccta gccgggtgga aaggtcgctg
      601 atgatctccc aatcctggcg tgcgtttccc acgggctcga tggctttcct gacccgctgc
      661 acgcgccgct cagtgttggt gaaggtacca tctttttcgg cgaaactgac tcccggaagg
      721 accacctggg ccagctcggc cgtttccgaa aggaaaaggt cctggaccac cagaaggtcc
      781 agatgctcca gttcccgccg gacttggttg ctgtccgggt cgctcaaaag ggggttttct
      841 cctaggatga agagggcttt tattttccct tgcccgattc ccttcatcat ttcgatgagg
      901 gtcacgcccg gtttcgggct caggggaacg gcccaggctt tttcgaactt ctgccgcacg
      961 gcctcatcat ccacccgctg gtacccggtg tacacgttgg gcagcccgcc catgtcgcag
     1021 gccccctgca cgttgttctg ccctctcaga ggattgaccc cggaatgggg ctttccaact
     1081 tgcccggcgg ccatggccag attggccagg gccaggacat tgttggtccc cgttgcgtgc
     1141 tgggtgattc ccatggcgta cagaatggtc gagttcggcg actgggcgta gagacgggcg
     1201 gcctccacca ggtccgccgc cggaaccccg gtgatccttt ccaccgcctc gggggtgtac
     1261 tcctttaccg cctcggccac cgcttcgaac ccctcggtcc gatttcggac gaaatcctcg
     1321 ttctgccagc catgggaaag gatggtttga atcatcccgt tgatcagggc cacgtccgtc
     1381 cccggtttgt ggtttaagaa cagatgggcg aactggacga gctcgatccg gcgggggtcg
     1441 gccacgatca gtttcgttcc gttatgccgt actgcggcct tgacctcgag cccgatcacc
     1501 gggtgattct cggtggtgtt ggacccgatg atcaggatgc agccggcctt gcggatctat
     1561 tcgatggggt tggtcatggc cccggagccg aatgccatcg ccagaccggc gacggtggag
     1621 ctgtgtcaga gccgggcgca gtggtcaacg ttgttggtcc ccagggcggc ccgggccatc
     1681 ttctgcagga ggtaattctc ttcgttagtg cacttggccg agaccaggac cccgatcgct
     1741 tccggtccgt acttggcctt caccctctta aggccttcgg ccgcccggtc cagggcttcg
     1801 tcccaggagg ctggctgaag cttgccatcc ttgcggatca ggggctcgat aagccggtcc
     1861 ggactgtgga cgtagtcgaa cccgaacctt cccttgacac aggtccagcc ccggttcatc
     1921 cccaggtctt tatccgaggt taccctgagt atctggttct ccttgacgtt cagaaccagg
     1981 gtgcatccca ctccgcaata cgaacagatg gttgggactt tcttcaaatc ccattcccgg
     2041 gcctgaaaac gcccggcttt ttcttccagc gctcccaccg ggcaaacact cacgcaattc
     2101 ccgcagaaga cgcagggtgt ctcctgcatg gagtggtcga aggaggcggc aatcttggtg
     2161 tcaaaccccc tcttcgattg gtccacggct tcgcagtact gaacctcgtg gcagaccgta
     2221 acgcatcggc cgcagagaat gcacttattg tagtcccgct cgaagaaggg attggccgcc
     2281 cgaagggagt aatcatgctt ttcccccagg aaacggtgct ggcggaggcc gaattggtag
     2341 gcatatttct ccagcaggca cgaaccgctc ttctcgcagg tcatgcagtc gtagggatgg
     2401 tccgagagca ggaattccaa cactagctta cgggcatcct ggactctttt cgtattcgtc
     2461 ctcaccacca tccggttggc caccggataa gcgcaagaag ccaccagcgt ccttgccccc
     2521 tccacctcca ccacgcataa acggcaggcg gtggtggggg taaggtcttc ccggtagcag
     2581 agatgcggaa tatggacccc ggccttccgc gctgcctcaa agatggaggt gccttctccc
     2641 gcccttactt cccgcccatc aatggtcaat tgtacctcgg ccatagttct tcctttcgcc
     2701 ccttacctct ttaacccttc aggtcgcagc gcaggcagcg ttgggcttcc cgcaccgcgg
     2761 tctcctcgtc gaagcccaaa ttcacctctt caaaagtgca gacccgccgg tccaccgggt
     2821 tcgccggcat ctgcgccctt ggtttggccg cttccttttc ttcctccccg tcctcgacct
     2881 tgggcacctc ggcccgccgc agaggctggt agacccggtt ctggcgtaag tcctgcccct
     2941 tcaagaagcg gtcgatggaa acggaggcct ggtagccccc ggccatggcc tccaccacgg
     3001 tggaaggacc cgtgaccaca tctcctccgg cgaagacccc ggggatatgg gtggccatgg
     3061 tgaccgggtc caccgacagg gtattcgctt tggtgatctg aaaaccgtct ccctggggca
     3121 ggcaggatag gtcggggata tacccgatgg ccggaataac ccagtcgact tccagaataa
     3181 actccgatcc cttcaccgga accggacggc gccggccggt ggcatcgaaa tcgcccagtt
     3241 ccatccgctg gcattccaag ccttctacct ttccgttccg ggccacgatc cgggtggggg
     3301 cgacgaggta taagaacctg atcctctcgt gctccgcctc ttgaatttcc tcatcgttcg
     3361 ccggcatctc ttcgcgggtc cgccggtaaa cgatggaaac ttccctggcc cccaggcgca
     3421 aagcggtccg ggcggcgtcg aaagccacgt ttcccccgcc gaccaccgcc acctttcccc
     3481 cgagttccac tttctccccg agattgaccc tccgcaggaa gtcgattccg ggcaggaccc
     3541 ccggtgtttc ctccccgggg atgttcaatc tctgattccg gtgggctccc agggctaaaa
     3601 agatggcttt gtatcccccc cggagcagtt cctcgaggct gaaatctctg cccagggctt
     3661 tgccggtctg aatttccacg ttatgctggc agatgaaacg caggtcgtgt tccatgattt
     3721 cccggggcag gcgataatcg ggaatgccca cggcgagcat tcccccggcc acgggcaagg
     3781 cctcaaacac ggttacccga tatcctcgcc tccccaactg gtaggcggcg ttcagacccg
     3841 caggccctgc cccgaccacc gccacccgat cctctttcat cttttccaga aaagcgggag
     3901 gggccaattt cccctgcttc cgttcccaat cggaagcaaa tcgcttcaag gaagcgatgg
     3961 ccagaggttc gtcgatctcc ccccgcttgc acatgttttc gcagggatga tcacagaccc
     4021 ggccgcagat gccggccagg ggcatgttta gacggatgag gctcaaggcc tcctcgtgcc
     4081 ggccttcccc gatgagggcg gcatatccca cggcgtcgca gcggaccggg caggtatttt
     4141 cgcaagggga aaagaccagg gcttcgcaag cgccggcggg acagaatttc ccccggatgt
     4201 gggcttcata ctcttgccgg aagtggtgaa tcgtggagag caccggattg ggacacgtct
     4261 gcccgaggcc gcaaagggag gcatccttga tgatcgggcc catctccagg aggagttcga
     4321 tatccccttc ctgtccctct ccccgggtga ttcgggtgag aatctccagc atgcgtttgg
     4381 tcccgatccg gcagggcacg cattttccgc aggattcatc ctgggtaaag ctcaggaaga
     4441 aacgggcaat gtccaccatg caggtcttct cgtccaggac gatcatcccg cccgacccca
     4501 tcatcgatcc cgcctgggtg agggactcgt agtcgatggg aaggtccagg tattgcgggg
     4561 gcaggcaccc tccggagggt ccccccatct gggcggcttt gaaacggcgg ccccgcggga
     4621 ttcctccccc gatgtcgtag atcacctgcc gaagggtggt gcccatgggc acctcgacca
     4681 acccggtgtt gttgatcttc cccgtaagag agaagatctt tgtgcccttg cttcccgccg
     4741 taccgacggc ggcgtaggcc tctgcccctc ccaggatgat ggacctgatg ttggcgtagg
     4801 tctccacgtt attgatgttc gtgggcttgc cccagagacc ggctatcgcc gggaaggggg
     4861 gccgcgcccg gggcattcct cttttcccct cgatggaggc caggagggcg gtctcttctc
     4921 cgcagacgaa agctcccgcc ccttctttga tcttcaggtg aaagccgaag gaagaaccca
     4981 gcacgttgtc tcccaacagg cccagttcct ccgcctgctc gatggccagg gtgagatgtt
     5041 gtaccgccag gggatactcg gcccgcacgt atacatgacc gctctgggct ccgatcgcgt
     5101 aaccggcaat gagcatgcct tccaagacca gatggggatt cccctccagc acgctccggt
     5161 ccatgaaggc tccggggtcc ccctcgtcgg cgttgcagac gaggtacttt ccctttcccg
     5221 gagcctggct ggtgaaacgc cactttcgcc ccgtgggaaa tccggcgccc ccccgtcccc
     5281 gcaatcccga cctttctact tcctcgacga cttggtccgg ggtcatggag gagagaactt
     5341 tcgccagggc ggaatacccg ccgcgggcga tgtaatcccg gatctccgtg ggatcgatcc
     5401 tcccgttgtc gctgaggacc atacgtttct gacgccggta aaagggaacc tcatgatcgt
     5461 aggtgattac ctggccggtg gccgggtcgg tgtagagcag gcgttgcacc acctgcccct
     5521 tggccagggt ctgggcgacg atctccggga catcgtccac cacgacctgc tggtaaaaaa
     5581 tctccttcgg gagaataacc accaccggcc cccgctcgca gaagccgtgg catccggtgc
     5641 gcatgacatc cactttttct ccgaggcttt gtttctggat ttcctgaatg aaggcgatcc
     5701 gaagttcctc ccctccccag gcccggcacc ccgttcctcc gcaaatggtg acgcaagtct
     5761 tctgcggatc ccagccccga agtatgcttt cgcggaagga tgtcaaatcc ttggccgatc
     5821 taagggtttc cttcaacttc cccctcctct ggaatactct ttcaggactc gatccacttt
     5881 cgcgggggtg agttttccgt aatacttctt gtccaccagg agaaccgggg acagcgcgca
     5941 agcccccaga caggcaacgg tttcaaaggt gaactcgtaa tccggagtcg tttcattctc
     6001 tcccaccccg agaatctgct ggaccgcctt cagaatgccc ttgcccccgc gcacgtggca
     6061 ggccgttccc cggcaaaccc gcaccgtatg tcggccccgt ttggtagtat aaaattggga
     6121 gtagaaagtg accaccccat aaacccggct tatggaaatt ttcagccttt ggctgatggc
     6181 cttcaggacc ccttccggca aatacccata gagggcttgg gcctcctgga ggacggggat
     6241 cagggcgcct tcctgccccc ggtacttctc cagaatgggg tccagcttgg aaaagtcgat
     6301 ctcttccggc atcgttccct ccactgcgca attcaacttc tcggcccggg gcgacccaaa
     6361 taccttggcc gggctgtccc cgcccgattg cgtattctcg gaatcggccg gcgaaagaac
     6421 aggatatgaa ctgcatccgc tggcaaattc aggcaccgga tagcagggcg ctatcccgcg
     6481 aactcccctt gtctttcttc tccgccggcg aaggatcctg gctggtccta cttcacccgt
     6541 cggtcatagg tcttttcgat cgctctcacc agattggtga tcgtccggtt gatcggggtg
     6601 tcgatcccca atctttctcc ttctcgcacg atggccccgt tgatgaaatc cacttcggtc
     6661 tcccgctggg ccaaaacgtc ctggagcata gaggaataat tcggcttggt ggcaatacat
     6721 gcgtcttcca cctttttcac cgcgtcggta taggggaggc gaatcccttt cgcctgggcc
     6781 acccgctttc cctcttccac caggcccctc atgatttccc gcgccgccgc ctgatccacc
     6841 aggactccgt tggccaggcg ggtgatcgcc gtgagagggt tcacccccgc gttcaccagc
     6901 agtttccccc aaacgtcgtt ccagacttcg gacgagacat gagttgaaag gccggctttg
     6961 ttgaaagcgg ccgcgatttt ctccgccctc gggctcgggc ctccccgcat ctccccgatg
     7021 accgtttctc cccggcccgc gtaaagcatc cggcccggcc ccaggtaagt ggaggcgttt
     7081 agagtcacac cggccaggat ccgtccttcc cccacgacag cctcgatggc ttccgggttg
     7141 cccaggccgt tctgcacagt cagccccacc gtctccggcc cgaataggca cctggcgctt
     7201 tgggccgcct cccgggtctg ccgggacttc ggcaggaaaa tgaccaaatc gactttcccg
     7261 acctcctcgg gtttcagggt tgcgttcttg acggggatgg atacctcttt tccttcaggc
     7321 tcgacgatcc ggatccctga actgcggatg gccgatacaa tatcttcctt gatatcgacg
     7381 gcgtaaacct cttccccgct ccgggagagg tacccggcat acagagagcc caacgctccc
     7441 gtgccaacag ctgcgattct catgaattaa atccccttcg atttctattg ctggttgctg
     7501 gttccttgtt gcccaaaccc agccccggag aatggccggt aacgagtaac cggaaaccag
     7561 taaccagcaa cgagttagga caagagacga tccacaatcc ctcccacgga ttggaggaaa
     7621 gtcggatttt cctgataagg gggggtcccc gaaaggtcgg cttccactac ctttgaatca
     7681 taatcgatga agcccaaaaa gggcaaaccc ggcattttct ccagcaggaa tt
//
LOCUS       DASPRR010000177         5951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1490474, whole genome shotgun sequence.
ACCESSION   DASPRR010000177 DASPRR010000000
VERSION     DASPRR010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490474"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VEL68_06935"
     CDS             <1..424
                     /locus_tag="VEL68_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase, dissimilatory-type subunit
                     alpha"
                     /protein_id="HXZ35733.1"
                     /translation="CMKWDEKAQSLTIEDADCNRCMHCINLMPKALRQGKEKGATLLI
                     GGKAPILQGALLSTVIVPFMKMEPPYNELKELISKIQDWWDENAKSRERIGELINRVG
                     MRTFLKVIGLKPVPQMVKNPRSNPYVFFWPEEIKKGGK"
     gene            427..1488
                     /gene="dsrB"
                     /locus_tag="VEL68_06940"
     CDS             427..1488
                     /gene="dsrB"
                     /locus_tag="VEL68_06940"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HXZ35734.1"
                     /translation="MAKTDIGPPDYKKMLPPVIQKNYGKWKYHEILKPGLLKHVAESG
                     EELYSVRAGSPRLLSTDHIREICDIAEKYCDGYLRFTSRHNIEFLLTDKSKVEPLIAD
                     LKKKNYPVGGTGHSVTNIVHTQGWVHCHTPATDASGPVKAVMDELIGYFESMTLPSQV
                     RIALACCLNMCGAVHCSDIAILGIHRTVPKPDHNRVPNVCEIPTTVAACPTGAIRPDP
                     KTKSVTINEEKCMYCGNCYTVCPALPLADPDNDGISIWVGGKVSNARSAPMFSKLAIP
                     FLPNNPPRWPETVEAVKKIVETYAKNARKHERIGEWIERVGWERFFRLTGIEFTDKHI
                     DDFTMAPGTYRTTTQFKWG"
     gene            1488..1718
                     /locus_tag="VEL68_06945"
     CDS             1488..1718
                     /locus_tag="VEL68_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory sulfite reductase D family
                     protein"
                     /protein_id="HXZ35735.1"
                     /translation="MMNAETKTKVLEWFQDKSKGEKKKFYIKDVVKGLPQEDRHAIQD
                     VVKELLDEGTLKYWSSGSTTYLMLAEYFPKEE"
     gene            1728..3134
                     /locus_tag="VEL68_06950"
     CDS             1728..3134
                     /locus_tag="VEL68_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HXZ35736.1"
                     /translation="MAGFLSRLVMAALRGGAGKTTLSLGMIAAWKRLGRNVAAFKKGP
                     DFIDSAWLSLAAQRPCRNLDTFLLGPKKVQQSFGRNAESRGISLIEGNRGLYDGMDSR
                     GTQSTAELAKLLKAPVVLVLDCAKVTRTLAAMVLGCQRMDPEVDIRGVILNQVARSRH
                     EKILRASIEETCGLPVLGAVPRMDDFPFPERHLGLIPPQEHQWVSRALERAAEVAEKY
                     LDLEPLWKIAETAPPFCWKPEEKRPILKAAGNQPEIGIMKDSAFQFYYPENLQALADR
                     GARLIEISAVREKTLPSLDALYIGGGFPETHAEQLADNLSFRQSLREAIENGLPVYAE
                     CGGLMYLGESLAMDTRIFPMAGVLPVSFSMEKRPQGHGYTLLEVERENPFYAVGRVLK
                     GHEFHYSRVRSLEEGRIHFAFRVKRGTGIKENQDGLWYKNVLATYSHVHALGCEEWAD
                     GLIRKAMESRSGYSMAAV"
     gene            3136..3951
                     /locus_tag="VEL68_06955"
     CDS             3136..3951
                     /locus_tag="VEL68_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXZ35737.1"
                     /translation="MEDLKMAAAVMRSVTGGNAENLARMEVLVREASRRGAHVVCFPE
                     MNISGYGLRQEMRSFAESIPGPSTEAILKMAREYDIVILAGLAEKAEKDRYFISHYAA
                     GPEGLIGIYRKIHLGPPEEGVFQAGSECPVFSCSRARFGIELCFDGHFPELSTLLALK
                     GAEVIFIPHASPRESSAEKKGRWMRYLPARAYDNSVFLVACNLLGETESGFVFSGSAF
                     ILDPKGEVLAESQNGGEGIILAELKSDTLRRVKENSKGFFLGRRRPEIYNKLA"
     gene            4097..4417
                     /locus_tag="VEL68_06960"
     CDS             4097..4417
                     /locus_tag="VEL68_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HXZ35738.1"
                     /translation="MPTVNLAGKAYEVDEDGFLQELDKWNADFASAYAKEEGIEGILT
                     EEHWKVINYLREYYAKFGIAPMIRKLCKDTGFDLKKIYDLFPSGPAKGACKLAGLAKP
                     TGCV"
     gene            4724..5137
                     /locus_tag="VEL68_06965"
     CDS             4724..5137
                     /locus_tag="VEL68_06965"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXZ35739.1"
                     /translation="MDLITLKHEKGLCFSVQVRGHRFLVDMPRESKGQDQGPSPSDLL
                     AASLGACMGMHMALYSQTAGLSCEGMEMNLVYNLVEEQGRKRIHTVTIDVALPKDPGE
                     RSAALLRAAQNCIVRNTLEKGPVIDVEITTKYSSE"
     gene            5464..>5951
                     /locus_tag="VEL68_06970"
     CDS             5464..>5951
                     /locus_tag="VEL68_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol oxidoreductase"
                     /protein_id="HXZ35740.1"
                     /translation="MTYRVTVWAKSPVPFRIPTTCGQQKSLPWIKSRPLESPHSTTGV
                     IGRMILEVLLFRSLFRNSKVELREGNPMYGGAKWLWLAGLAFHWSFLIIVFRHLRFFF
                     EPVPGCVNLLATLDGLFEIGVPNLFLTDGLLLGSVTYLFLRRVAIPYLRYISLPADYF
                     PLL"
BASE COUNT         1371 a         1596 c         1754 g         1230 t
ORIGIN      
        1 gtgcatgaaa tgggatgaaa aggcccagag tctgaccatc gaggatgccg actgcaaccg
       61 ctgcatgcac tgcatcaacc tgatgcccaa agccctccgc cagggaaaag aaaagggggc
      121 aacccttttg atcggcggca aggcgcccat cctgcagggc gccctgcttt ccacggtgat
      181 cgttcctttc atgaagatgg agccgccgta caatgaattg aaagagctga tcagcaagat
      241 ccaagactgg tgggatgaaa acgcaaaatc ccgcgagagg atcggggagc tgatcaaccg
      301 ggtggggatg cggactttcc tcaaggttat cggactgaag ccggtgcccc agatggtgaa
      361 aaatccgcgg tccaacccct acgtgttctt ctggccggaa gaaatcaaga agggaggaaa
      421 ataaccatgg ccaagaccga catcgggccc cctgactata aaaaaatgct gccccccgtg
      481 attcagaaga attacggcaa gtggaagtac cacgaaatcc tcaagcccgg cctgctgaaa
      541 cacgtggccg agtcgggaga ggaactctac agcgtaagag cgggttcccc ccggctgctg
      601 agcaccgatc atatccggga aatctgcgat atcgccgaga aatactgcga cggctatctc
      661 cgcttcacca gccggcacaa catcgagttc ctcctgacgg acaagagcaa ggtggaaccc
      721 ttgatcgccg acctgaagaa gaagaattat cccgtaggcg ggaccggcca ttcggtgacc
      781 aatatcgtcc acactcaggg ctgggttcac tgccacactc cggccacgga tgcttccggc
      841 ccggtgaaag cggtcatgga cgagctgatc ggctacttcg agtccatgac ccttccctcc
      901 caggtgcgga tcgcgctggc ctgctgcctg aacatgtgtg gagccgtgca ctgctcggac
      961 atcgccatcc tggggatcca ccggaccgtt cccaagcccg accacaaccg ggttcccaac
     1021 gtgtgcgaga ttcccaccac cgtggcggcc tgccccaccg gggcgatccg tcccgacccc
     1081 aagaccaaga gcgtgaccat caacgaagag aagtgcatgt attgcggcaa ctgttacacg
     1141 gtctgcccgg ccctgcccct ggcagacccg gataacgacg gcatttccat ctgggtcgga
     1201 ggcaaggtgt ccaacgcgcg gagcgcacct atgttctcca agttagccat tccttttctg
     1261 cccaacaatc cgccgcgttg gccggagacc gtggaagcgg tgaagaagat cgttgagacc
     1321 tacgcgaaaa atgcgcggaa acacgagcgc atcggggagt ggatcgaaag ggtcggttgg
     1381 gagaggttct tccgcctgac cgggattgag ttcaccgaca aacacatcga tgatttcacc
     1441 atggctccgg ggacctatcg gaccaccacc cagttcaagt ggggttgatg atgaacgccg
     1501 aaacgaaaac gaaggtcctg gaatggttcc aggataaatc caaaggggag aagaagaagt
     1561 tctacatcaa ggacgtggtc aaggggctcc cccaggaaga ccgccacgcc atacaggatg
     1621 tggtgaagga acttctggat gaggggaccc tgaagtactg gtccagcggc agcacgacct
     1681 acctcatgct ggccgagtat ttccccaaag aagaatagcc tgaggccatg gccgggtttc
     1741 tttctcggtt ggttatggcg gcattgcggg ggggagcggg gaagaccacg ctttccctcg
     1801 gtatgattgc cgcctggaag agacttggcc gaaacgtggc cgcctttaag aaagggccgg
     1861 atttcatcga ttcggcctgg ctgtcgctgg ccgcgcagcg accctgccga aacctggata
     1921 ccttcctcct ggggcccaag aaagtccagc agtccttcgg gcggaatgcc gaatcccggg
     1981 gaatatcctt gatcgaagga aaccggggtc tctacgacgg aatggactct cggggcacac
     2041 agagcacggc ggagctggcc aagctgctga aggctccggt ggtgctggtc ctggactgcg
     2101 ccaaggtaac ccggaccctg gcggccatgg tcctgggatg ccagaggatg gaccccgaag
     2161 tggacattcg cggagtcatc ctgaaccagg tggcgcgcag ccgtcatgaa aaaattctgc
     2221 gggcatccat cgaggaaacc tgtggactcc cggttctagg ggccgtgccc aggatggacg
     2281 attttccgtt tcccgaaagg catctgggct taatccctcc ccaggaacac caatgggttt
     2341 cccgggccct ggagagagca gcggaagtcg ccgaaaaata tttggacttg gaaccgctct
     2401 ggaagattgc ggaaaccgct cctccgttct gctggaaacc ggaagaaaaa agaccgatcc
     2461 tgaaggctgc agggaaccag ccagagatcg ggatcatgaa agactcggcc ttccagtttt
     2521 attacccgga gaacctccaa gccctcgccg accggggagc ccggctgatt gaaatcagcg
     2581 ccgtccggga gaaaaccttg ccgtcgctgg atgcgcttta cattggcgga ggatttccgg
     2641 agacgcacgc cgagcagttg gcggataacc tctctttccg ccaatctttg cgggaggcaa
     2701 tcgagaacgg gttgccggtt tatgcggagt gcggcgggct catgtacctg ggagagagcc
     2761 tggctatgga tacccggatt tttccgatgg ccggagtcct gcccgtgagc ttctccatgg
     2821 aaaagcggcc gcaggggcac ggttacaccc ttttggaagt ggaaagggaa aatcccttct
     2881 acgctgtcgg ccgggttctg aagggtcatg agtttcatta ttcccgggtt cggtccctgg
     2941 aggagggccg gattcatttt gccttccggg tcaaacgggg cacggggata aaggaaaatc
     3001 aagacggcct ctggtacaag aatgtcttgg ctacctattc tcatgtccac gccctgggct
     3061 gcgaagaatg ggcggatggg ctgattcgaa aagcgatgga gagccgatcc ggctattcca
     3121 tggcggcggt ctgagatgga agacctgaag atggccgcag cggtcatgcg ctctgtcacg
     3181 ggcgggaacg cggagaacct ggccaggatg gaggtcctgg tccgggaagc gtcccgccgg
     3241 ggagcgcacg tggtctgctt cccggaaatg aatatcagcg gctatggact caggcaggag
     3301 atgaggtctt ttgccgaatc catacccggg ccttccaccg aagccattct gaaaatggcc
     3361 agagaatatg atattgtgat cctggcgggg ctggcggaga aggcagaaaa ggatcggtat
     3421 ttcatcagcc attacgccgc cgggccggag gggctgatcg ggatttaccg aaagattcac
     3481 ctgggtcctc cggaagaggg ggtctttcaa gccgggtcgg agtgtccggt tttttcttgc
     3541 agcagggccc gattcggaat cgaactctgt tttgacggcc acttccccga gctcagtacc
     3601 ctgcttgctc ttaagggggc ggaggtcatc tttattcccc acgcgtctcc gcgggagtct
     3661 tcagccgaga agaaagggcg atggatgcgt tatctgccgg caagagctta tgacaacagc
     3721 gttttccttg tagcctgtaa tttgctggga gagaccgagt ccggctttgt tttttcgggc
     3781 agcgccttca tcctggaccc caagggggaa gtgctggcgg agagtcagaa cgggggagaa
     3841 gggatcattc tcgccgagct caagagcgat acgctccgca gagtaaaaga gaattcgaag
     3901 ggattcttct taggacgaag acgcccggaa atatacaaca aactggcata gagcaaaggc
     3961 caattaacaa tgatcaatga gcattgacca ttggctattg atcattgatc attgagtata
     4021 cactctctcc ttcttctccg gggcggagga atcgaagggg tgtcatacta aaaatatcaa
     4081 ctaaggagga acaaggatgc cgaccgtcaa tctggcaggg aaagcttacg aagtagatga
     4141 agatggtttc ttgcaggaac tggacaaatg gaatgcggat ttcgccagtg catacgccaa
     4201 ggaggaggga atcgagggga ttttgaccga agagcactgg aaagtcatca actacttgag
     4261 ggaatattac gccaagttcg gcatcgcccc catgatccgc aagctctgta aagacacggg
     4321 gttcgacctg aaaaagatct acgatctctt cccctccggc ccagccaagg gagcctgcaa
     4381 actggctggc ttggccaagc cgacagggtg cgtgtgagga attggcccgg aagggccaaa
     4441 tgactctgag ttattggttc gaagtagagg cgggtttgaa acccaccgat acagcatttg
     4501 caggggcacg ccgcagcgtg cccccgcgca taggttgcca gaaaagcaat tcccccgtct
     4561 tatttaagag aatatccagt ttttagtttc cggctgccag gttttggttt ccgaccggca
     4621 acggggaacc aggaacggac aatgtgagcc gctccagccc attttcgtcg ctttttactt
     4681 aaggcactta agctcactta agtacacttg agacactttt cacatggacc tcatcaccct
     4741 gaaacacgaa aaaggtcttt gcttttccgt tcaggtccga gggcacaggt tccttgtgga
     4801 tatgcccagg gaatcgaagg gccaggacca gggaccttcc ccctccgacc tgctggccgc
     4861 ctccctgggg gcctgtatgg gcatgcatat ggccctctac agccagaccg ctggtctctc
     4921 ctgcgagggg atggagatga acctggttta caacttggtg gaagaacagg gtcgaaaaag
     4981 gatccatacc gtcaccatag atgtagccct tcctaaggat cccggcgagc ggagcgcagc
     5041 gcttctgcgg gcagctcaaa actgcatcgt ccggaacacc ctggagaagg ggccggtgat
     5101 cgatgtggag ataaccacga aatacagttc ggagtaatat agttcgaagt tcggaaagaa
     5161 acagcctcta tcgaatcacc aggaatggct cgatttgaat gcacgaatca accatctatc
     5221 tctcctgggc atcaaccttt actccgagtg ccgggagaaa gtgaatcagc taaggatcag
     5281 ggattgggga aaaagggcag gagcggatca agatcggagg gttgaagtct gaatctcgga
     5341 gtttctctcg ctgccgttat ccttctttct ctaattgctc ttggcggagc gggcgggata
     5401 ggggcctatt ctcttttcgg aatcgtcatc ccctatgcgg ctttcatcgt ctttctggcg
     5461 ggggtgacgt atcgggtcac ggtctgggcc aagtccccgg tccctttccg catccccacc
     5521 acctgcgggc agcagaaatc cctgccctgg atcaaatcca gaccattgga gagccctcac
     5581 agcacgacag gagtcattgg aaggatgatt ttggaggtac tcctgttccg ctcccttttc
     5641 cggaactcca aggtcgagct gagagaagga aacccgatgt acgggggggc gaaatggctc
     5701 tggctggcag ggcttgcctt ccattggtct tttctcatca tcgtcttccg ccatcttcgt
     5761 tttttcttcg aacctgttcc cggctgcgtg aatcttctgg caactctcga cgggttattt
     5821 gaaatcgggg taccaaatct ttttttgacg gatggcctgc tcctgggttc ggttacttac
     5881 cttttcctgc ggagagtggc catcccttat ctgaggtaca tctcccttcc ggcggattat
     5941 ttcccgcttc t
//
LOCUS       DASPRR010000178        12115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_47710, whole genome shotgun sequence.
ACCESSION   DASPRR010000178 DASPRR010000000
VERSION     DASPRR010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12115)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47710"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VEL68_06975"
     CDS             complement(<1..548)
                     /locus_tag="VEL68_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXZ35741.1"
                     /translation="MKTDEVVIREVMLRDGLQNIVDFIPTEAKIELFRLIVASGLKSV
                     EVTSFVSPKAIPQFRDAAQVVQATAKMTPKGVEVSALIPNLKGAQLALESGMHKVGLV
                     MSVSESHNINNVRRTTAESIDELKRVLELRGKYPDLIVKAGMATVFGCPFEGKIKPEV
                     TLGFIRQFYKLGIRDMSMADTVG"
     gene            complement(633..1808)
                     /locus_tag="VEL68_06980"
     CDS             complement(633..1808)
                     /locus_tag="VEL68_06980"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ35742.1"
                     /translation="MPLSHIKVLDLTRFLAGPFCAMLLADMGAEVIKIETPEHGDDAR
                     YQGVVVKGESGYFVGMNRNKKALTLDLKSPEGKKIFLRLAEKADVVVENFRPGVMRNL
                     GLDYETLRKVNPGIVYCGISGFGKDGPHALRPAFDFIAQGISGFMSLTGFPDREPVRT
                     GIPISDSVAGIYGAYGILVALMARQRTGRGQEVQTSLVDAMISVMSLQADWYFHTGKV
                     AERAGNDHPVGAPYGTFKALDGYINIAPAGQPMWERLAQALGLKDLISDPRFATNDLR
                     RENRKQLNEIINRITSQKPMKEWIDYLNREGVPCGPIYNLAQTFEDPQVKHQEMMLEL
                     EQPSVKMKVLGFPVKMSETPARIRRPSPQLGEHSEEILRNLGYSQDEIRELRGKKVI"
     gene            1969..3159
                     /locus_tag="VEL68_06985"
     CDS             1969..3159
                     /locus_tag="VEL68_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ35743.1"
                     /translation="MATPVRVGVVGAGFAAAFHLRCYRQVQGVPVEVAAIVDINKARA
                     EELAKRHGVSKVFNDFRFVLEDKSIQVIDLVVPNSLHLPMVLEAAKAGKDIICEKPLT
                     GYFGEGEPEEKVGTTSKRKMLKKVQEDLQMVSKVMAGHTAKFCYAENWVHAPAFKKAL
                     ELMEKCFTSGSILEIRGEESHSGSHSEFAKWWRYSGGGALARLGAHPLASGLFLKHFE
                     GEKKYGKPILAKEVWADGTDLTKTPAFANAKGSPLVTGWGDVETWSQITVTFTDGTRG
                     TFFAADDLLGGMESNMDVRLSNARIKFDLCPVRVCQFYTIDPAPVKDVYFNEKQETKA
                     GLSFPNPHEEWLLGYPHEIQDFMEAIAFNRQPLTGLELAKQVVEVMYAGYVSMEEGRR
                     ISIS"
     gene            3364..4152
                     /locus_tag="VEL68_06990"
     CDS             3364..4152
                     /locus_tag="VEL68_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35744.1"
                     /translation="MKKIFGFIKAHYHKPLLVVAFFTLWQLLVMLFNVKEYIVPSPIS
                     VFARLFVPEMASNYHWLKHIRATLVEIFFGFAVTASLGIFLALVISWSNLFRQIITPL
                     LTFFNSIPKIAMAPLFILWFGYGIVPNILIAFLIAFFPVVINTAAGLNAVEEDLLDLV
                     RYLHARKWQVFMKVRFPNSLPYIFSGLKISSTLCVVGAIVGEFIASNQGLGYLLKDSQ
                     AAIDTPPMFAGLILISLLGMGLFGILTACERIFMPWQKVEQKSG"
     gene            4163..4951
                     /locus_tag="VEL68_06995"
     CDS             4163..4951
                     /locus_tag="VEL68_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35745.1"
                     /translation="MKKILESLKTHYQMPVTILGFFLVWQFSVMVFKVREFILPPPLT
                     AILHLILPQADSTYHWWVHIKITLQEIFISFAVTAVVATWIATLICWSRSIRNFVLPF
                     FVFVNSLPIIAVGPIFLLWLGYGVMTNILIAFLVSFFPIVINMATGLDEVEEDLLDLV
                     RYLHASKWQIFIKIRFPNALPYLFSGLKISSTMCVVGAIVGEFIASDKGLGYIIINSQ
                     FTLDTPAIFAALIVISLMGFGLFGLVALGESLFMPWYDPGKEKG"
     gene            4997..6013
                     /locus_tag="VEL68_07000"
     CDS             4997..6013
                     /locus_tag="VEL68_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35746.1"
                     /translation="MKRKVFIAVAGLMALVALSIPGAEAAEKKLQKTSLILNWKVVGD
                     HSPYFVAMKKGWFAEEGLDVDVILGQGSNFSVQTVDTGKAVFGIADAPVAIAGRAKGA
                     KVKVIGIIFDKHPNCMYWWKESGITGPKDLVGRKVGVPAADGHKVMFPAFAKIINIDP
                     SKVEFVNIEPAAKAAILATKKVDVIFDLYTGKPFIEKVVPPEKLGYAIWADYGFNAYA
                     HAYITSDATIEKNPELVKAFLKVSYRAWEFTLKNPEEAIKTLAEYHPINQQDYLANLK
                     LVREFFKTDRYKSKGIGYIDPARMKDTIDIVDRVMGVKVTFKPEDAYSAAFLPTPPYK
                     LPEF"
     gene            6067..6831
                     /locus_tag="VEL68_07005"
     CDS             6067..6831
                     /locus_tag="VEL68_07005"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35747.1"
                     /translation="MEAKKPLISFQGVRKTFSTRTGDLEALGGVTFSVYPGEFVSIVG
                     PSGCGKTTLLKILAGLLPRSGGEIEVDKEQFDPAREVGFVFQKSLLLRWRKVIDNVLL
                     PVEILGDDRSAKWKRAQELLELVGLKGFENSYPHELSGGMQQRVSIARALIHDPKVLL
                     MDEPFGALDALTRERMNLELLRIWTEAKKTVLFVTHGITEAIFLSDRVIVLSARPSRM
                     VSALDIPLPRPRTLEGRTTPEFGKLSLQVYNSLEIM"
     gene            6900..8258
                     /locus_tag="VEL68_07010"
     CDS             6900..8258
                     /locus_tag="VEL68_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ35748.1"
                     /translation="MSHDKDIRVEQAKIAFEDFSYRAKIKFGGKVVSQMTLLNVEVEV
                     SGRKGSRARGRGSMPLGNVWSFPSKIYSQDDTLGTMKRMATIFCPLTEEYREFGHPVE
                     INHFLEQGYLKAAGKLEKEIGLAERIPKLCTLVTASPFDAAIHDAYGRLHGMNVYRTY
                     SESFMNYDLSFYLGPAFAGQYLDRFTLGEPKARMPLYHLVGALDPLTALESGAQIPDG
                     HPVTLSEWIRQEGLTHFKIKLNGDDLAWDLNRMVSVDKVCRQEIGGQREWVYSADFNE
                     RCPHVDYLVEILKKFAEETPAGFSRLQYIEQPTSRYLQDVPEQNMTHASSLKPVVIDE
                     SLTDLDSLKLAIKLGYSGVALKACKGQSQSLLMGAAAQAENLFLCVQDLTCPGASFLH
                     SASLSARIPGVVAIEGNARQYCPEANRGWEKKYPGLFVIRDGTVETGVLTGPGLGFDY
                     QD"
     gene            8346..9566
                     /locus_tag="VEL68_07015"
     CDS             8346..9566
                     /locus_tag="VEL68_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ35749.1"
                     /translation="MKEDLFHLPVKIGDVQLRNPFIVASGPTAKRVDQLEQAEKCGWG
                     AASIKQTFNPLPYINYQPRYRWLKKDKLHVFTAEYRLAMEQGLRLVEEARKKCKDLVI
                     IANYSYVAGQDIDGWLEAARRFDAAGAQILELNFCCPNMSFNVDISERAKKDVRPSSG
                     ASMGQDEDAVRWVIENTRRVTSLPLIAKITPEGGRISEVSKVAFDSGAAAVCSVANRL
                     GIPAIDVWNYKKPIYNLQGQNTMACLSGPWIKPLALRDVFEIRKLVGPGPHINGTGGV
                     ASMEDAVQMMMCGADSIGICTETMISGFGFLEKWMKSMKDYMKKMGFKTVRDIRDLLL
                     SEIKPASELTVWAGYAQVDSKKCSKCGLCVEIGHCNAVVMTDESASIDSKLCQGCSTC
                     IDICPKKAIQMVGG"
     gene            complement(9700..11001)
                     /locus_tag="VEL68_07020"
     CDS             complement(9700..11001)
                     /locus_tag="VEL68_07020"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXZ35750.1"
                     /translation="MRYLVIAGLLGLVFAIGAAQVTEAPTGFDNNSNGTVTDAIHQAD
                     RETFDNTENVEDGLGPLYNAQSCRECHQNPTSGGASQVTELRVGHLGPDGRFRNPEIP
                     IARGAEIISGRSLVNDRAICPNEEFPNTEIQERVPVTENIRTLRLSLNLLGDGFVEAV
                     ADQTLIELARKQCAASHGKICGQVLQVPIVEAPGKTGVGRFGWKNQQASLLSFSGDAY
                     LNEMGITNRLFPEEVTKLCNTVSEPNDTPGPDGLEDIDRFARFIRATKAPARDAVLAE
                     TPKTMRGSKLFDQISCSTCHVRSLTTAPAGTQINGGTFTIPEALGQKTFHPFGDFLLH
                     NVGTGDGIVMAWQEHYGPTVYKYQWKDLSKDAFEKTRNKVRTAPLWGVRLRPRLMHDG
                     ASETFLDAISRHRGEANEARLGFERLSRGDKEALYEFLRSL"
     gene            complement(11338..11751)
                     /locus_tag="VEL68_07025"
     CDS             complement(11338..11751)
                     /locus_tag="VEL68_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ35751.1"
                     /translation="MEWHRDEFVISDDLNRVQVEVVADLLAKTFWGHRRPREVVEKLI
                     QTSICFSLFHQQWQIGFSRVVTDYAVFSWLSDLVLNEGYRGRGLGGWLIGCILEHPQI
                     SGTQFVLQTTYAAGLYKKFGFQANEKLMTRTPSTW"
BASE COUNT         2712 a         3276 c         3218 g         2909 t
ORIGIN      
        1 ccaaccgtgt cggccatgga catgtctcga atccccagct tataaaactg gcggatgaaa
       61 ccaagagtta cctccggttt gattttgccc tcaaaaggac agccgaagac ggtggccatc
      121 ccggctttca cgatcaggtc cggatatttt cccctgagtt ccaggaccct tttcagctca
      181 tcgatcgact ctgccgttgt ccgcctgacg ttattgatgt tgtgggattc gctcaccgac
      241 atgaccaacc ccaccttgtg cattccgctc tccaaagcca gctgagcccc cttcagattc
      301 gggatcaggg ccgatacctc cactcccttg ggggtcattt tcgccgtggc ctggaccacc
      361 tgggcggcat cgcggaattg agggatggct ttgggactca caaaggaagt aacttccacg
      421 ctcttcaggc cgctggccac gatcaggcgg aaaagctcaa tcttcgcttc cgtggggatg
      481 aaatcgacga tattctgcag accgtcccgc agcatgacct cacgaatgac aacctcgtcc
      541 gtcttcatca atccgctcct ttcctcttgt cccctcggcc tgatcccccc cgcggaaaac
      601 ggatttaacg ggagaatccc tcgcctcggg aatcagatca ccttcttgcc tcttaactct
      661 ctgatctcat cctgggagta cccaaggttc ctaagaatct cttccgagtg ctcgcccaat
      721 tgcggggatg gacggcgaat tcttgccggg gtctcgctca tcttgacggg aaaacccagg
      781 accttcatct ttaccgaagg ctgctccaat tccagcatca tctcctggtg cttgacctgg
      841 gggtcttcga aggtctgggc caggttgtag atgggcccgc aggggactcc ttcccggttc
      901 aggtaatcaa tccactcctt catgggcttc tgggaggtaa tccggttgat gatctcgttc
      961 agttgcttac ggttttccct ccttaaatca tttgtagcaa accgcgggtc cgaaatcaaa
     1021 tccttcaggc cgagggcctg ggccagcctc tcccacatgg gctgcccggc gggggcgatg
     1081 ttgatgtacc cgtccagggc cttgaaggtt ccgtagggag cacccacagg gtggtcattt
     1141 cccgctctct cggcaacctt gccggtgtga aaataccaat ccgcctggag gctcatcacg
     1201 ctgatcatgg cgtccaccag gctggtctgg acctcctgcc cccggccggt tctctgtctg
     1261 gccatcaagg cgaccaagat cccataggcg ccgtaaatcc cggcgaccga gtcggagatg
     1321 ggaatcccgg tccttaccgg ctcccggtcg ggaaacccgg taaggctcat gaaccccgag
     1381 atcccctggg cgataaaatc aaaggctggg cgaagggcgt gggggccgtc tttcccgaac
     1441 ccggagatcc cgcagtagac gatgcccggg ttcactttcc tgagcgtttc ataatcgagc
     1501 cccaagttgc gcataactcc gggccgaaaa ttttccacca ccacatcggc cttttccgct
     1561 agccggagaa aaatcttttt cccctccggg gatttcaggt ctagcgtcag ggccttcttg
     1621 tttcggttca tcccaacgaa ataaccgctt tcccccttga cgacgactcc ctggtatcgg
     1681 gcatcgtcgc cgtgctccgg cgtttcgatc ttgatgactt ccgcacccat atccgccaga
     1741 agcatggcgc aaaagggacc cgcgaggaac cgggtcaggt cgagaacttt aatatgcgaa
     1801 aggggcatga ctctcctcca ttttgccgtc attttaaaga agtagattga tttgtcaagg
     1861 agaaaaacca ggattcgaat tcatcttgac aaatatatcc ccgccattta gcgttataat
     1921 accatcaaga atagcgcttt tattttatcc ggaaaggaag atggatcgat ggctacgccc
     1981 gtaagagtcg gagtcgtggg ggcgggcttc gcggccgcct ttcatttgcg ctgctaccgc
     2041 caggtccaag gggtcccggt tgaagttgcg gcgattgtgg atatcaacaa ggctcgggcc
     2101 gaagagttgg ccaagcgcca tggagtttcc aaggtcttta acgattttcg gtttgttctg
     2161 gaagacaaat ctattcaggt catcgatctg gtcgtcccca attccctcca ccttcccatg
     2221 gtcctggagg cggccaaagc gggcaaggac attatctgtg aaaaacccct gaccggctac
     2281 ttcggagagg gagagcccga agagaaggtc gggaccacat ccaaaaggaa aatgctcaag
     2341 aaagttcagg aagacctcca aatggtgagc aaggttatgg ctggccacac ggctaaattc
     2401 tgctacgccg agaattgggt ccatgcacct gcctttaaga aggccctgga actgatggaa
     2461 aagtgtttta cctccggcag tattttggaa atacgggggg aagagtctca cagcggctct
     2521 cactctgaat tcgccaagtg gtggcgatat tcaggcgggg gagccttggc ccgcttgggc
     2581 gcgcatccct tggccagtgg tctcttctta aagcatttcg agggagaaaa gaaatacggc
     2641 aagccgattt tagccaagga agtatgggcg gatggaacgg acctgacgaa aacgccggcc
     2701 ttcgccaatg cgaaaggcag tccattggtc acgggatggg gagatgtgga gacttggtct
     2761 cagatcaccg tgacctttac cgacggtacc aggggcactt tcttcgccgc tgatgacctt
     2821 ttgggcggaa tggagtccaa catggacgtc cgtctgtcca acgctcgcat taaatttgac
     2881 ctctgcccgg tgcgggtctg ccagttctac acgatcgatc ctgctccggt gaaagacgtc
     2941 tatttcaacg aaaagcagga gaccaaggcg ggcctctcct ttcccaaccc ccacgaggag
     3001 tggctcctgg gctatcccca cgagattcag gacttcatgg aagccatcgc cttcaaccga
     3061 cagcccctga caggattgga attggcgaaa caggttgtcg aagtgatgta tgctgggtat
     3121 gtatccatgg aagaaggacg aagaatttcc atatcgtgag gggtgaggga tcaaagaaat
     3181 atcctcacct ctcaattccc acctccgggt ttaggtcatt gaactttgaa atttggaatt
     3241 tgtctgggat ttggagaacg gataaatgcg ggcaatgaac tttcagcctt agatggccca
     3301 gaaaacacct ttggattaca atacatcggt attggttggg caataagtgc ctaattcccc
     3361 tttatgaaaa agatatttgg ttttatcaaa gcccattacc acaaacccct tctcgttgtt
     3421 gcttttttta ccctctggca gctattggtc atgctgttca atgttaagga atacatcgtg
     3481 ccttcaccca tctccgtatt tgcccgtctg tttgtaccgg aaatggcctc caattaccac
     3541 tggctgaaac atatccgagc tacgctggtg gaaatctttt tcgggtttgc cgtcaccgcc
     3601 tctctgggga tttttctggc tttggtaatc tcgtggtcta acctcttccg gcaaatcatc
     3661 acccctctct tgaccttttt caattcgatt cccaagatcg ccatggcccc gctctttatc
     3721 ctttggttcg gatatggcat cgttcccaac atcctgatcg cttttctgat tgcttttttc
     3781 cccgtggtca tcaacaccgc agccggactc aatgcagtgg aggaggatct cctcgacctg
     3841 gtccggtatt tacatgcccg caaatggcag gtttttatga aggtccgatt tcccaactcc
     3901 ctcccgtata tattctccgg cctgaaaatc tcctcaaccc tttgcgtggt cggagccatt
     3961 gtgggcgaat ttattgcctc gaatcaagga ttgggttacc tgttaaaaga ttcgcaggcg
     4021 gccatcgaca cccctcccat gtttgccggc ctgatcctca tttctttgtt gggcatgggc
     4081 cttttcggga tcctgacagc ctgcgaaagg atattcatgc cctggcagaa ggtggaacaa
     4141 aaatcaggct gatgggaaac ggatgaaaaa gatattggaa tctctaaaaa ctcattacca
     4201 gatgcccgtt accattctag gtttcttcct ggtgtggcaa ttttcggtca tggtgttcaa
     4261 ggtgcgggag tttatccttc ccccccctct gacggcgatc ctccacctga tcttgccgca
     4321 ggccgattcg acctatcact ggtgggttca tataaaaatt actcttcaag aaatattcat
     4381 cagcttcgcg gtgaccgcag ttgtggctac ctggatagcc acgctcatct gctggtcgag
     4441 gtccatccgg aatttcgtgc tccccttttt cgtctttgtg aattcccttc cgattattgc
     4501 cgtgggtcct atttttctcc tctggcttgg ttatggggtt atgaccaata tcctcatcgc
     4561 tttcctggtt tctttcttcc caatcgtgat aaacatggcc acaggcttag atgaggtgga
     4621 ggaagatctc ctcgacctgg tgcgttattt acatgccagc aaatggcaga tttttatcaa
     4681 aattcgattt cccaatgctc tcccctacct tttctccggg ttaaagatca gttccaccat
     4741 gtgtgtggtg ggagcgatcg tgggggaatt tatcgcttcc gacaaggggt tgggatatat
     4801 tatcattaat tctcagttta ccctcgatac gccggcaatt tttgcggccc tcatcgtgat
     4861 ttccctgatg ggttttggac tgttcggctt ggtcgcgctg ggggaaagcc tattcatgcc
     4921 ctggtatgac ccaggcaagg agaaggggta atagaaattt catccccgaa atattatcca
     4981 taggagggag gacccaatga aaaggaaagt gtttatcgca gttgctgggc tcatggcttt
     5041 ggtagccctg tcgattccgg gggcagaggc ggctgaaaag aaactccaga aaacttctct
     5101 catattgaac tggaaagtcg tcggggatca ttctccttac tttgtcgcca tgaagaaagg
     5161 gtggttcgcc gaagaaggcc tggatgtgga tgtgatctta gggcaggggt ccaatttttc
     5221 ggtccagact gtggatacgg gaaaggccgt ttttggcatt gccgacgctc cggtggcgat
     5281 cgcgggcaga gcgaagggcg ccaaggtaaa ggtgatcggc attatcttcg ataaacaccc
     5341 caattgcatg tattggtgga aggagagcgg gatcacgggg ccgaaggacc tcgtcggaag
     5401 gaaagtggga gttccggcgg ccgatggtca caaggtgatg tttcccgctt ttgccaagat
     5461 tatcaacatc gacccctcga aggtcgaatt tgtaaatatc gaacctgccg ccaaagcagc
     5521 catcctggca accaagaaag tggacgtcat ttttgatcta tacaccggta aacccttcat
     5581 tgaaaaagta gtccctccgg aaaaactggg atacgccatc tgggcggatt acggcttcaa
     5641 tgcctatgcc catgcttata tcacctcgga tgccacaatc gaaaaaaatc cggaactggt
     5701 gaaggcattt ttgaaggtct cttaccgggc ctgggaattt acgctcaaaa atcccgaaga
     5761 agcgattaaa accctcgccg agtatcatcc catcaaccag caggattatc ttgccaacct
     5821 gaagctggtc cgggagttct ttaagactga ccgttacaaa agcaagggga tcgggtatat
     5881 tgatccggcg cggatgaaag ataccattga tatcgtcgac cgggtgatgg gggtaaaggt
     5941 gacgtttaag cctgaagatg cttactctgc tgcctttttg ccgacccctc cttataaact
     6001 tcccgaattc taggaacccc ttggaggggg gcttccccac ctgttcccga aaagtggaga
     6061 gaacctgtgg aggccaagaa accgctcatc tcttttcaag gtgttcggaa aacgttttcc
     6121 acccgcacag gtgacctaga ggccctgggt ggggttactt tcagtgttta tccgggcgag
     6181 tttgtttcga tcgttggccc ctccggttgt gggaagacca cgcttttgaa aattcttgct
     6241 ggccttcttc ccagaagcgg aggggaaatc gaagtggata aagaacaatt tgaccctgcc
     6301 cgggaggttg ggtttgtttt tcagaaatct ctattgcttc ggtggcgaaa agtcatcgac
     6361 aatgtgcttc tgccggtcga aatcctcggg gatgaccgtt cggccaaatg gaagagagct
     6421 caggaactcc ttgagctggt aggtttaaaa ggttttgaga acagctatcc tcacgaacta
     6481 tccgggggga tgcagcagag ggtctccatt gcccgggctc tcattcatga cccaaaggtt
     6541 ttgctcatgg acgagccctt cggtgcactg gatgcgctga cccgggaacg aatgaacctc
     6601 gaactcctgc ggatatggac ggaagcaaaa aagaccgtgc tgtttgtaac ccatggcatt
     6661 accgaggcga tcttcctctc cgatcgggtc attgtcctct ctgcccggcc ttcccggatg
     6721 gtctccgccc tggatattcc gcttcccaga ccccgaaccc tggaagggag aacgaccccg
     6781 gagtttggaa aactctctct acaggtttat aattccctgg aaatcatgta agggacgatc
     6841 ctgcttccat ttcaggcatt aggaaaagag accgccagac gtttgatgtg gaagaaacca
     6901 tgagtcacga taaagatatt cgagtggagc aggccaagat tgcttttgag gatttttctt
     6961 accgggccaa aataaaattt ggagggaaag tggtatccca gatgaccctc ctaaacgtcg
     7021 aagtagaggt atcgggccgc aagggaagca gggcccgagg gcggggctcc atgcctctgg
     7081 gaaatgtatg gtcttttcca agcaagattt atagccagga tgataccctc gggacgatga
     7141 agaggatggc tactatattt tgccctctca cggaggaata cagagagttt ggccacccgg
     7201 tggagattaa ccacttcctc gagcaaggat atttgaaggc cgcggggaag ctggagaaag
     7261 aaatcggtct cgcggaaaga attcctaaac tatgcacact ggtgacggcc agtcctttcg
     7321 atgcggccat ccatgatgcc tacggccgcc ttcatgggat gaatgtttat cgcacctatt
     7381 cagaaagctt catgaattat gacctttcct tctacctggg ccctgccttt gccggtcagt
     7441 acctggaccg gttcaccctc ggggaaccaa aggccaggat gcccctttac catctcgtag
     7501 gggcccttga ccccctgacc gctctagagt ctggggccca gatccctgat gggcatccgg
     7561 taaccctatc ggaatggatc cggcaggagg ggttgaccca ttttaagatt aagctaaatg
     7621 gggacgactt agcctgggac ctaaatcgga tggtttcggt ggataaagta tgtcgccagg
     7681 aaataggcgg acagagagaa tgggtttaca gcgctgattt caacgaaagg tgtccccatg
     7741 ttgactacct ggtggaaata ttaaaaaaat tcgcagagga gacccccgca ggtttttctc
     7801 gcctgcaata tatcgaacaa ccgacctccc gttacctgca ggatgtcccg gagcagaaca
     7861 tgacccatgc ttcttccttg aaacccgtag tgatcgatga atccttaacc gatcttgatt
     7921 cgctgaagct agccatcaaa ctaggatatt ccggtgtcgc cctcaaggcc tgcaagggac
     7981 agtcccagtc cctcttgatg ggggcggccg cccaggccga aaatcttttc ctctgcgttc
     8041 aggaccttac ctgtcccggg gcctctttcc tgcactctgc aagtctcagt gcccggattc
     8101 ctggcgtggt ggccatagag ggaaatgcca gacaatactg cccggaagca aacagaggct
     8161 gggagaaaaa gtatcccgga ctttttgtga tcagggatgg aacggttgaa acaggggtgc
     8221 tgaccggccc ggggctaggt tttgattatc aagactagaa ctgtctcaat ggatttgatt
     8281 tctttggccc atattcacct tccttggaga tttgggattt aaatttctcg cgggagagtc
     8341 tgaccatgaa agaagatttg ttccaccttc cagtcaagat cggcgacgtc caactccgga
     8401 atcccttcat tgtcgcttcc ggccccacgg ccaagcgtgt ggatcagctg gagcaggccg
     8461 agaaatgcgg ctggggcgcg gcttccatca agcagacctt caatcccctc ccttatatta
     8521 attatcagcc tcgctaccgc tggctaaaaa aggataaact gcacgtcttc accgccgaat
     8581 accgcttggc catggaacag gggttgcgtc tggtggagga ggcccggaaa aagtgtaagg
     8641 acctggtcat catcgccaac tactcttacg tggcggggca ggatatcgat ggttggctgg
     8701 aggcggcccg ccggttcgac gcagccggcg cccagattct cgaactgaac ttttgctgtc
     8761 ccaacatgtc ttttaacgtg gacatctccg agcgggccaa gaaggatgtc cgtccttcct
     8821 ccggagccag catgggacag gatgaggatg cggttcgatg ggtaatcgaa aacacacgaa
     8881 gggtcaccag cctgcctttg atcgccaaaa tcacgcccga agggggccgg atttccgagg
     8941 tatccaaggt tgcttttgat tcaggagcgg cagcggtatg cagcgtggcc aaccggctgg
     9001 ggattcccgc catcgacgtc tggaactaca aaaagcccat ctacaacctc cagggccaga
     9061 ataccatggc ctgcctatcc ggtccctgga ttaagcctct cgcgctgcgg gatgtcttcg
     9121 aaatccgcaa actggtaggc cccggccccc acatcaacgg cacaggtgga gtcgcttcca
     9181 tggaagacgc cgtgcaaatg atgatgtgcg gtgccgattc catcggtatc tgcaccgaga
     9241 ccatgatcag cgggttcggc ttcctggaaa agtggatgaa gtccatgaag gattacatga
     9301 agaagatggg cttcaagacc gtcagggata tccgggacct tctcctctcg gaaatcaagc
     9361 ctgcctctga attaaccgtc tgggctggct acgcccaggt agactccaag aaatgttcta
     9421 agtgcggcct gtgcgtcgag atcggccact gtaatgccgt tgttatgacc gacgaatcgg
     9481 cgtccattga ttcaaagctt tgccagggct gctctacctg catcgacatc tgccctaaaa
     9541 aggccattca gatggtggga gggtaaacgg gaaccgggca aagcagggag acattcgtga
     9601 atggatgaat ttttcttggc ggggggggat tcctttcggg atcatccccc ctcaccttag
     9661 ccggttgtcg tcgcccaggg aacgcgccca cctccgactt cacaaagacc tcaggaattc
     9721 atagagggct tctttatccc cgcgcgagag cctttcaaag ccgagcctgg cctcgttcgc
     9781 ctcgccgcga tgccgcgaaa tggcgtcgag gaaggtctcc gaagcgccgt cgtgcatcaa
     9841 ccgggggcgc aatcgaacgc cccacagggg cgcggtgcgg actttgttcc tcgtcttctc
     9901 aaaggcgtcc ttggacaggt ctttccattg gtatttgtac acggttgggc cataatgttc
     9961 ttgccaggcc atcacgatgc catcgccggt tcccacattg tgtaacaaga aatcaccgaa
    10021 ggggtgaaag gtcttttggc ccaaggcctc ggggatggtg aaggtaccgc cattgatctg
    10081 ggtgcccgca ggagcggtgg tcaaggatcg gacatgacag gtggagcaac tgatctggtc
    10141 aaagagtttg gacccgcgca tggttttggg tgtttccgcg agcacggcat ctcgggcggg
    10201 agccttcgtg gcccggatga aacgggcgaa ccggtcaata tcctcgagcc cgtccggacc
    10261 cggcgtgtcg ttcggctctg acaccgtatt gcagagtttg gtcacttcct ccgggaacag
    10321 tcggttggta atgcccattt cattcaggta ggcgtctccg gagaaggaaa gcaggctcgc
    10381 ctgctgattc ttccatccaa accgccccac gcccgttttg cctggagctt ccacgatggg
    10441 aacttggagc acctggccgc aaatcttgcc gtggctggcc gcgcattgtt tgcgcgccaa
    10501 ttctatcagg gtttgatccg ccaccgcctc cacgaagcca tcccccagaa gattgaggga
    10561 aagccgcagg gtacgaatgt tttccgtcac gggcacacgt tcttgaatct ccgtgttcgg
    10621 aaactcctcg ttgggacaga tggctcgatc attcaccagc gagcggccgg aaatgatctc
    10681 ggctccacgg gcaatgggaa tctcgggatt gcggaagcgc ccgtcgggcc cgagatggcc
    10741 gacgcgcagt tccgttactt ggctcgctcc acccgaggtc ggattctggt ggcactcgcg
    10801 gcaggattgc gcattgtaaa gggggccaag cccatcttcc acgttctcgg tattgtcgaa
    10861 ggtttctcgg tcggcttggt ggattgcatc agtcaccgtt ccattgctgt tgttgtcaaa
    10921 acccgtaggt gcttctgtta cctgggcggc cccgattgcg aaaaccaacc ccagcagccc
    10981 cgcaatgacc aaatacctca tggcttcccc tccttcctgt tcgaccattt gttggacagg
    11041 ctatctgctt ctctgagcct tttcccttat tgatccttca acaaacagcc gagatgatcg
    11101 gaatctatct ctctggccac ggcggcgcgt ggattcgttg gcgccgggac aaggccgggt
    11161 gacaggatgg gtactatcct taaggaatcg tccttacgag accatctata ttttacatta
    11221 tatcccattc caacagatgg gttaatagga ataaatgggg caaatccttt atttttgggt
    11281 acatggggac attatatttc catgcccgag gacaaccaag caacgctctt caattcctca
    11341 ccatgtgctc ggcgttcggg tcatcaattt ctcattcgcc tggaatccga atttcttata
    11401 caaaccggcc gcatatgtag tctgaagcac aaactgcgtc ccggagatct gcgggtgctc
    11461 taggatgcac ccgatcagcc atccccccaa tccccttccc cggtagcctt cgtttagaac
    11521 caggtcggac aaccaggaga agaccgcata atccgtcacc acccgggaaa aaccgatctg
    11581 ccactgttga tggaaaaggg agaaacaaat ggaggtttga atcaactttt caacaacttc
    11641 ccgcggtcgc cgatggcccc aaaaagtttt agccagcagg tcggccacaa cctcaacctg
    11701 aacccggttc aggtcatctg aaatcacaaa ctcatcccga tgccattcca ctcgattctc
    11761 ctcggtcagc agccacctaa gtcatgacct ccaccatagg tggaggcatg aaatacccct
    11821 gaaccgctca aagtcgatga gacccgaccg cgggcggatt taaatgagcg ccacctggag
    11881 gtggccatcg tctggacgcc aatgccaggc ccgggggcaa gggtctgggt ttcaaaaatc
    11941 atgaaacgtc cgtcctttca tgggcccggt cggccaaccg tctcttccac ggcgtgggag
    12001 gtcttgtcct tgtaggggcg accggccggt cgccctttcg ggccgcctcc tgtaccggaa
    12061 gccgagccca gcatcgagta accgggatcg ccacgccgat gctttttgaa cccca
//
LOCUS       DASPRR010000179         8041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_47970, whole genome shotgun sequence.
ACCESSION   DASPRR010000179 DASPRR010000000
VERSION     DASPRR010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47970"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..791
                     /locus_tag="VEL68_07030"
     CDS             462..791
                     /locus_tag="VEL68_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35752.1"
                     /translation="MGNRRNGGTIFCSCLLIFVVLGTIGAGGLKKGSDEMEVTGKVYV
                     MGNEPFTHVAIKMDDGQVYALVGEHEKELRRLQGRRILATGRPSEEKPRGTKAIEVKS
                     FRVLEEK"
     gene            863..2683
                     /locus_tag="VEL68_07035"
     CDS             863..2683
                     /locus_tag="VEL68_07035"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HXZ35753.1"
                     /translation="MKRYFPIGLVLSAILFFASGWVGPLSAASEKAPISGTPMLREQN
                     LRFLRDYGFSARIKPEKGILADTVGTTVLFWAFDFGASSYYQTSATCRSITPLSTGFN
                     LNIYVEDAIFNPSLFTTAILDSIANEYKNVILPTETAYFGTPPTGDFTILLMDIKDSG
                     GGTFVSGYFDQLNENSGVTHSNNRHMIYMDSIQGTPGSTSFLGTLAHEFQHFIHFNYD
                     PSEESWVNEGLSGLARFVCGYGHQISHVTAFSQVPTTSLTFWQSELANYGATYLFMLY
                     LTEKYGGAATTKAIVANSGTGITGINNALFSRGLPVGVNDIFKNWVVANYLNNSSISS
                     GSYGYSASFSGITNAPGNITMTATVSTYAATGSGSVNQYAADYVKFTNLGGTYDIFVL
                     IPYSLTESAAQSYSYTGTIGSFNLSLSGLSATLPAEGVKQGTSNPTPAVVTSLSSSNT
                     ISTGEGGGNSSGGGGGGGCFIATAAYGSPLAEEILTLQEFRDRFLLTHLPGRVLVKVY
                     YRLSPPLADFISEHEGLKFMTRVALYPFVGLSRSILQTPKEVGFCGIGLFLLVGLILI
                     GGRNTQRGSAAPQRNLSLPPGRLFRNSSCPRKRASRKSEC"
     gene            2822..3736
                     /gene="gspC"
                     /locus_tag="VEL68_07040"
     CDS             2822..3736
                     /gene="gspC"
                     /locus_tag="VEL68_07040"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HXZ35754.1"
                     /translation="MMKFLTRHFWVVHVLFIACAAWLVSHISILFIEDRVLSLPRANA
                     SKKSPPLARSAPEPYDKYSPVTERNIFSPADRGQKLLPLEERKASLPGSRMTEAGQVA
                     PTGGYILAGTITGPGGYSWAILQEKGTRKQQIFRIHGNIGGGKIVSVTRKQIQIEKDG
                     KREILTLSEEETQPRASGKPLPQAPLPPAGQEEVKKLSANRFLVNREDVNAAVGDINR
                     FMTQARLKPHFEVGKPAGYSVSEIVPGSLMEKLGLKNNDIIKKVNGIGVSRPEEIMQA
                     YAQLQKDSNIELEIDRGGRTEILRYEIR"
     gene            3806..5842
                     /gene="gspD"
                     /locus_tag="VEL68_07045"
     CDS             3806..5842
                     /gene="gspD"
                     /locus_tag="VEL68_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HXZ35755.1"
                     /translation="MKELIGRFMLAFFFLGVITLVPGWVWHAQGQEKKGTEIQMPPKP
                     PPEDALITMDFQEADLNVVIKFMGELTGKNFLVSDGVRGKVTIISPKKITVREAYKVF
                     ESVLEMNGYTAVPGDDAIKIIPSAVARQMGLEIREGKEAKDVTPEDRMITQVIPLEHA
                     SAEEVRNLLASSVSKEGMIISYKPTNHLIITDRASNIHRLLRIIEQIDVQIVEEKISV
                     FPLENAAARTLADKLTQLMAVDPRQTAAAGRPPAVPGVARLVRIIPDERTNTLVVLAN
                     MQDTEEVRKLVLQLDKTAPRGKSQLHVRYLEHARAEDLAKVLSTIAAAKSKAVARTQP
                     GQPGAPQVEEASITADKATNSLVITASPQEFNELEEVINQLDTLRSQVLVEALITEVS
                     LEKALQIGVDWRIMDQPVEGSVRGFGGSDFGLISGVQQGTLPSSAGNTGLLLGLAKGF
                     ITIGGVQVPNIGVLVRAFQQDTDSNVLSTPHILTLDNEKAKIIVADNVPLIKSDVAAP
                     LVSTQTATSVAISRTFEYKDIGIQLEITPHISKGNIVRLEISTEVSNILSIDPANPGF
                     VTTRKRQATTTVAVESGQIIVIGGLIRDESSEVTKKVPCVGNVPILGWLFKNLSGTKT
                     KTNLLIFISPQIVRTAEDLERATSKKKMESDENLEKLKKERENEVKDTFDKLVK"
     gene            5976..7709
                     /gene="gspE"
                     /locus_tag="VEL68_07050"
     CDS             5976..7709
                     /gene="gspE"
                     /locus_tag="VEL68_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HXZ35756.1"
                     /translation="MTKEKNAASSGSSGMLDRIVAEIVQQRKGIDSAALEETQQKRNG
                     KPLSSWIVQKGWMTEEDLLTELAVRIGVPYWKDLEAKGLESSLVSRVPIAFAKRNGIV
                     PVKLEEEVLTAATSDPLNHEALDDLRLILGCREVRAILSSEQEVLRAINRFYERGGET
                     PEEMIQSLDTESSDRILHELEETEDLLEVSDEAPVIRLVNLILFQGVKERASDIHIEP
                     FQKELKVRYRIDGILYNRLSPPKRYQPAIVSRLKVMAKLDIAEKRLPQDGRIPIKIAD
                     KDIDIRVSVIPTTFGERIVLRLLNKSSAHFGLEEIGLSPGHLKALEELIQRPNGILFV
                     TGPTGSGKTTTLYAALSRINSSDKNIVTIEDPVEYQLFGIAQIQVNPKIGLTFAHGLR
                     SILRHDPDVVLVGEVRDPETAEIAIQAAMTGHLVFSTLHTNDAASAVTRLLDMGIEPF
                     RIASVVRAIAAQRLVRVLCPDCKEGYSLEAGVLRDTGIFPAQLRGRRVYRAKGCPACA
                     GTGYRGRTGIYEIMLVSEPIRQLIMKKADSATIARQALEEGMKTLRDDGVEKVMAGET
                     ALEELVRVSQE"
BASE COUNT         1936 a         2228 c         2257 g         1620 t
ORIGIN      
        1 acaaaagtcc cgatcaccaa ttatggtctc tctatttcct tctcccaggg agtcatccga
       61 agagttctct ctccattccc cgcggccctc gaagcctttc agcgagagat ggaaaagatg
      121 cgccgaacgg taaagtctcc ccaatttctc aagtcctccc tttcttgaaa ggggagaatt
      181 acctacggct gcagggagta cctttaaaac cttccccggc gcaaaccggg tcgaaagaga
      241 cacgtcttaa ttggcataaa gcttgcatta taaaatttct ttacaatttg taaaaatagt
      301 cacataattc taaacggtta tcaggaaaat cagaaggtcg cctattcagc tttcctttat
      361 ttcctggacg agaatcctct tctggggcag gaaagaatgg gagataaggg atcatgggaa
      421 acccagaaag ggggaaatgg ccccctggat tcctttggat aatgggaaat cggagaaatg
      481 gggggaccat tttctgcagc tgtctactta ttttcgtcgt ccttggaacc atcggggcgg
      541 ggggcctgaa aaagggaagc gacgagatgg aagtcaccgg gaaagtctac gtaatgggaa
      601 atgagccgtt cacccatgtg gccatcaaga tggacgatgg gcaggtttac gctttagtgg
      661 gggagcatga aaaggaactc cgcaggcttc agggaaggcg aattttggca accggcagac
      721 cttccgagga aaaacctcga gggaccaagg cgatcgaggt gaagtcgttt cgggtactgg
      781 aggaaaaatg acctccttta tacccaaatt ttcttttcat tcatcgtccc ttaaataagc
      841 ctatccaagc tgagaggagg gcatgaaaag atactttccc atcgggttgg tcctgtcggc
      901 catcttgttc tttgcttcgg gatgggtcgg gcccctttcc gccgcttccg aaaaggcccc
      961 catcagcggc acgcccatgc tgcgggagca aaacctgcgg tttctcaggg attacggctt
     1021 cagtgcccgg atcaaacccg aaaaaggtat cctggcagat acggtgggca ccaccgttct
     1081 cttctgggcc tttgattttg gggcgtcgtc atattatcaa accagcgcca catgcagaag
     1141 cattacaccc ttgagcacgg gttttaattt aaacatctat gtagaagatg cgatatttaa
     1201 ccccagcctt ttcacgaccg caatcttgga cagcatcgct aatgagtaca agaatgtaat
     1261 tctcccgacg gagacggcct atttcggcac tcctcccacg ggagacttca ccatactcct
     1321 catggacatt aaagacagcg gcgggggtac cttcgtatcc ggctactttg accaactgaa
     1381 tgagaattcc ggggtcaccc acagtaataa ccgccacatg atctacatgg actcaataca
     1441 gggaactccg gggagtacct cttttttggg gaccctggcc catgagtttc agcatttcat
     1501 ccatttcaac tatgacccct ccgaagagag ctgggtgaat gaaggcctgt ccggactggc
     1561 ccgattcgtt tgtgggtacg gccatcaaat cagccacgta accgcctttt cccaggtccc
     1621 tacgaccagt ctgacctttt ggcagagtga gttggcaaat tatggggcga cctacctgtt
     1681 tatgctctat ctcactgaaa aatatggagg ggcggccacc accaaagcca ttgtggccaa
     1741 cagcggcacc ggaatcaccg ggatcaataa cgcccttttc tcgagggggc ttccggtggg
     1801 ggtcaacgat attttcaaaa actgggtggt ggccaattac ctgaacaatt cctccatctc
     1861 cagcggcagc tacggctact cggccagttt ctccggcatt accaatgccc ccggaaatat
     1921 caccatgact gcgacggtca gcacctatgc cgccacgggg agcgggtccg tcaaccagta
     1981 tgcagccgac tacgtcaaat ttactaacct gggtggaacg tatgacattt ttgtgctcat
     2041 cccatacagc ttgaccgaaa gcgctgcgca gagctactcc tacacaggaa caatcgggtc
     2101 tttcaacctg agcctgagcg gcctctccgc cactctgccc gccgaaggag tcaagcaggg
     2161 aacctccaat cccactccgg ccgtggttac gagcctttct tcttcgaaca ccatttccac
     2221 cggcgagggc ggagggaaca gcagtggagg gggaggtggc ggtggatgct tcatagccac
     2281 cgcggcctac ggctctccgc tggcagaaga gatattaacc ctgcaggagt tccgcgaccg
     2341 gttccttttg acccatctcc ctggccgggt cctggtgaag gtctactacc gcctcagccc
     2401 gccgctggcg gacttcatat ctgagcatga aggtttgaaa ttcatgaccc gggtggctct
     2461 ctatcctttc gtgggtctca gtcgttcaat cctgcaaacc ccaaaggaag tcgggttctg
     2521 tgggatcggc cttttcctcc tcgttggttt gatcctgatc ggtgggagga atacacagcg
     2581 tggcagcgcc gcaccccaaa ggaatctctc cctcccccct ggtagattgt ttcgtaattc
     2641 gtcatgcccg cgaaagcggg catccaggaa gtctgaatgt tgaataaatt caatactgga
     2701 ttcccgcttc cgcgggaatg actgttcaga acgaaaaaag agtttttcag catccttata
     2761 gaggcttttg gagaggggat ttaggggcga aaatgaggct gcgaaggttt aacttttgaa
     2821 gatgatgaaa tttctcactc gacatttctg ggtcgtccat gttcttttta ttgcctgcgc
     2881 cgcctggctc gtctctcata tctccattct cttcatcgaa gaccgggtcc tgagccttcc
     2941 ccgggccaat gcatcgaaga agtcgccccc actcgcaagg agcgcccctg agccttacga
     3001 taaatattct cccgtaaccg aaaggaacat tttcagccct gcggatcgag gccagaagct
     3061 tctccccctg gaggagagaa aggcctccct tcccggaagc agaatgaccg aggctggaca
     3121 ggttgccccc acgggcggct atattctggc cggtaccata accgggccgg gggggtattc
     3181 ctgggccatt ctgcaggaaa aggggacccg caagcagcaa atattccgaa ttcatggtaa
     3241 tatcggtggg ggaaaaatcg tcagcgtcac ccggaaacaa attcagatcg aaaaggatgg
     3301 aaaaagggaa atcttgaccc tttccgaaga ggagacccaa ccccgggctt caggaaaacc
     3361 gcttccgcag gcgcccctcc ctccggccgg gcaggaagaa gtaaagaaac tctcggccaa
     3421 tcggtttctg gtgaaccggg aagacgtaaa cgccgccgtg ggagacatca accgtttcat
     3481 gactcaagcc cggctcaagc ctcattttga agtgggtaag ccggccggct attcggtttc
     3541 ggaaatcgtt cccggcagcc tgatggagaa actgggtctc aagaacaacg acattatcaa
     3601 aaaggtcaat gggatcggtg ttagccgccc ggaggagatc atgcaggcct acgcccaact
     3661 ccagaaggac tcgaacatcg aattggagat cgacaggggc ggtcggacgg aaatcttgcg
     3721 gtacgaaatt cgctgacttt tttaaatccc gtagggggag gtcttacacc cgcctccgat
     3781 gtgatttttg gtagggagag cccttatgaa ggaattgatc gggcgtttca tgttggcttt
     3841 tttctttctg ggggtcataa ccctggttcc cggctgggtt tggcacgccc aggggcagga
     3901 gaaaaagggt acggaaatcc agatgccccc caagccccct cccgaggatg ccctgatcac
     3961 catggacttc caagaggcgg acctcaacgt ggtcatcaaa ttcatggggg agctgacggg
     4021 aaaaaacttt ctggtgagcg atggggtgcg agggaaggta acgatcattt cccccaaaaa
     4081 gatcacggtt cgcgaagcgt acaaagtctt tgagtccgtg ctggaaatga acggctacac
     4141 ggctgtgcca ggggacgatg cgatcaagat tatcccctcc gccgtggccc gccagatggg
     4201 cctggagatt cgggaaggaa aagaggccaa agacgttacc ccggaagacc ggatgatcac
     4261 ccaggtcatc cccttggagc acgcctcggc tgaagaggtg cgcaacctcc ttgcctcttc
     4321 agtctccaag gaggggatga tcatctccta taaaccgacc aaccacctta tcattaccga
     4381 tcgagcctcc aacatccacc gcctcctccg gattatcgaa cagatcgacg tgcagattgt
     4441 ggaggagaaa atcagcgtct tccccctgga aaatgcggcg gcccggactc tagcggacaa
     4501 attgacccag ctcatggccg tggacccgcg gcagaccgcc gccgcaggga ggccgccggc
     4561 cgtccctggg gttgcccggt tggtcaggat catccccgat gaacggacaa atactctggt
     4621 ggttctggcc aatatgcagg acaccgagga ggtccgcaag ctcgtcctcc agctggacaa
     4681 gacagccccc aggggaaaga gccagctcca cgtccggtac cttgagcatg cccgggcgga
     4741 ggacctcgcc aaggttttgt ccaccatcgc agcggccaag agtaaggctg ttgctcggac
     4801 tcagcccggg cagccgggcg ccccgcaggt ggaagaggcc tcgatcactg cggacaaagc
     4861 caccaattcc ctggtcatca ccgcctctcc ccaggagttc aatgagctgg aagaggtgat
     4921 caatcagctg gacacccttc gttcccaggt cctggtggaa gccctgatta cggaagtctc
     4981 gctggagaaa gccctgcaga tcggagtgga ctggcggatc atggatcagc cggttgaggg
     5041 gagcgtgcgg ggcttcgggg ggtcggattt cgggctgatc agcggagtgc aacaggggac
     5101 ccttcccagc agcgcgggga acacgggact cctattaggg ctggccaaag ggtttatcac
     5161 catcggcgga gtgcaggttc ccaacatcgg ggtcctggtt cgggcgtttc agcaggacac
     5221 ggattcgaat gtgctttcta ctccccatat cttgaccttg gacaatgaaa aggccaagat
     5281 catcgtggcc gacaacgttc ctctgataaa atcggacgtg gccgccccct tggtttcgac
     5341 ccaaacggcg accagcgtcg ccatctcccg cacttttgag tataaggaca tcggcatcca
     5401 gctggagatc accccccata tcagcaaagg aaacatagtc cgcctggaga tctccaccga
     5461 agtgagcaac atcctgtcca tcgacccggc caacccgggg ttcgtaacca cgaggaagcg
     5521 gcaggccacg acgacggtag cggtggagag cgggcagatc attgtgatcg gggggttgat
     5581 ccgggacgag tcctcggagg tgaccaagaa agttccctgt gtgggaaatg ttcccattct
     5641 gggctggttg ttcaagaacc tttcggggac taagaccaag accaacctcc tgatcttcat
     5701 cagcccccag attgtccgca cagccgaaga cctggagagg gccacgagca agaaaaagat
     5761 ggaatccgat gagaacctgg agaagcttaa aaaagagcgg gaaaacgagg tcaaggatac
     5821 ctttgacaaa ctggtcaaat gaacggtgaa caagcatctc aagtcaaaca ttgctaaagg
     5881 gccaatagac gggccgcctc ggagaggatg aggcgagggg gcttcttgag gggccccctg
     5941 aatgagcccc gaacccgctc ctaaggagcg cttgaatgac caaagaaaaa aatgctgcct
     6001 ccagcggctc ttcaggcatg cttgacagga tcgtcgctga aattgttcag caaagaaaag
     6061 ggatagattc tgcggcgctg gaagagaccc agcagaaacg gaacggaaag cctctatcca
     6121 gctggatcgt acagaaaggc tggatgaccg aggaggacct ccttaccgag ctggccgtcc
     6181 ggatcggggt gccctactgg aaagacctgg aagcgaaagg actggagtct tctctggttt
     6241 cccgggtgcc catcgccttt gccaagagaa acgggatcgt cccggttaaa ttggaggagg
     6301 aggtcctgac cgcggcgact tcggacccgc taaaccatga agccctggac gatctccggc
     6361 tgattctggg atgccgggag gtgcgggcga tcctcagcag cgagcaggag gtactccggg
     6421 cgatcaaccg cttttacgaa cggggcgggg aaactccgga ggaaatgatt cagtccctcg
     6481 acacggagag cagcgaccga atcctccacg aactggagga aaccgaagac ctgctggagg
     6541 tgagcgacga agcgccggtg atccgcctgg tcaacctcat cctttttcag ggggtgaaag
     6601 agcgggccag cgacattcac atcgaaccct tccagaagga gttaaaagtc cgctaccgga
     6661 tcgacgggat cctctacaac cgtttgagcc cacccaagag ataccagccg gccatcgtct
     6721 cccgcctgaa agtcatggcc aagctggaca tcgctgaaaa gcgccttccc caggacgggc
     6781 ggatccccat caagatcgcg gacaaggaca tcgacatccg ggtctcggtc atccccacta
     6841 cctttgggga aagaatcgtt cttcgcctgc tgaacaaaag cagcgcccac ttcgggctgg
     6901 aagagatcgg cctttccccc ggacatctga aggccctgga ggagttgatc cagcgaccca
     6961 acgggatcct cttcgtcacc ggccccacgg ggagcgggaa aaccacaacg ctctatgcgg
     7021 ctttaagccg gatcaattcg tcggacaaga acatcgttac tatcgaagac ccggtggaat
     7081 accagctttt cgggatcgcc cagattcagg tgaatcccaa gattggattg acctttgccc
     7141 acgggcttcg gtcgatctta cgccacgacc cggacgtggt cctggtggga gaggtgcggg
     7201 acccggagac cgcggagatc gccatccaag cggccatgac cggtcacttg gttttcagta
     7261 cccttcacac caatgatgcc gccagcgccg tgacccgcct cctggacatg ggaatcgaac
     7321 cctttcggat cgcttccgtg gtccgggcca ttgccgctca acgtctggtc cgggtcctct
     7381 gtccagactg caaagagggt tattccctcg aagcgggggt tcttagagac acgggcattt
     7441 tccccgccca gcttcgagga aggcgggtct accgagccaa aggctgcccg gcctgcgccg
     7501 ggaccgggta ccggggccgg acggggatct acgaaatcat gctcgtttcc gaacccatcc
     7561 gccaactgat catgaagaag gccgactcgg ccaccatcgc ccgtcaggcc ctggaagagg
     7621 gaatgaagac gctacgggac gacggggtgg aaaaggtaat ggcgggggag actgcgctgg
     7681 aagagttggt gagggtctct caggaataaa gaccaacggc cccgcgcaaa cagttaccgc
     7741 caaaccgcga cgagaaacca ataagcagca actaatacaa gcgcattaaa taaaatgaca
     7801 tcacccccac cctaaccctc ccctcttgag ggggagggga gggtgggggg taaaataata
     7861 tatttcgtct gtattagtaa ccaacaacca gcgccgaggg acgattcatt gcccgttttc
     7921 gaatacgaag gtcttagcaa ttcagggaaa gccctccgcg ggatcatcga cgcggagagc
     7981 ttgcgcaacg cccggagcaa gctgcggagc cagggggtct atcccacgga aatccgggaa
     8041 g
//
LOCUS       DASPRR010000180         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_198998, whole genome shotgun sequence.
ACCESSION   DASPRR010000180 DASPRR010000000
VERSION     DASPRR010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198998"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VEL68_07055"
     CDS             <1..183
                     /locus_tag="VEL68_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35757.1"
                     /translation="NLADSRALEEINCRDKDYRAVAVVVYTMEEKVVISDFKERGLDF
                     ILPDSIWEGLYKTLCR"
     gene            262..765
                     /locus_tag="VEL68_07060"
     CDS             262..765
                     /locus_tag="VEL68_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35758.1"
                     /translation="MISDASSSLMKDQGSLRYLIAFFSGFTAVLLFHQGVLSILHAVG
                     FTPVAPFPMQPTKPFGLPQIWSLAFWGGVWGLVLAGVDRRFPRGAGYWVAACIFGAIF
                     PSLVAWLVVFPMKGLPAGGGWHAAGLATGLMINGGWGVGTALLLQGLSKGWRSGGPSM
                     RPESPRI"
     gene            854..3535
                     /locus_tag="VEL68_07065"
     CDS             854..3535
                     /locus_tag="VEL68_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ35759.1"
                     /translation="MSLHSLKKVLSVLGLAPWLLCASSIWAENGGGEEEVLRATLENG
                     LRVVIVRNTLAPVVTTIINYMVGSNEAPEGFPGMAHAQEHMMFRGSPGLSANQLAGII
                     AVLGGRFNADTQQTVTQYFLTAPAEDLDVALHIEAVRMRGVLDSERLWAQERGAIEQE
                     VAQDLSNPEYVFYTKLLAHMFKGTPYAHTPLGTVSSFNQTTGAMLKQFYDTWYAPNNA
                     LLVIVGDVQTRKALEEVKKLFAGIPAKKIPERPPIQLEPVTAEAFPLKTDLSYGLVMI
                     SFRFPGYDSEDYPATQVLADVLSNQRGNLFALVPEGKALYAGFTLSPLPKAGLGYAVA
                     AFPKGADPSSLVKEVRRVLEEDAQKGFPADLVEAAKRNKLTQAELKKNSVFGLAMAWS
                     QALAVEGQSSPEEGVKAIQKVTVSDVNRVARKYLNLDQAVVAVLTPEPSGKPVPAQAH
                     RAVESFTPKATGPAKLPEWAEKSLGRISVPASRVNPQITVLPNGLKLMVQPESVSNTV
                     SVYGYVKNTPDLEAPQGKEGVDQVLNQLFSFGTTSLDRRAFQKALDDIGAKAAAGPDF
                     FVQALASEFELGVRLLADNVLRPALPEGAFKTVQRQAAASVAGLLESPEYLAERALKA
                     ALFPPNDPTLRQATPATISSLTLRDVKDYYRRIFRPDLTSIVVIGKVTAEEAKAVIEK
                     HFGSWTASGPKPETLLPPVAPSRPSSTAVPDQSRVQDKVTLAETLGLNRANPDYYALQ
                     LGNHVLGGAFYATRLYQGLREKSGLVYYVGSSFDVGQTRAIYMVNYACDPQNVSRARD
                     IVEQNLKRMHTAPVTPQELHQAKALLLREIPLSEASVDNIAQGLIYRASHELPLDEPT
                     RAAHQYLKLTAEHVQAAFARWVRPNDLVQITEGPTPK"
     gene            3890..>4347
                     /locus_tag="VEL68_07070"
     CDS             3890..>4347
                     /locus_tag="VEL68_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35760.1"
                     /translation="MNKRLLMSIVALAISLAFVSGGMAQQKPTPAQPTPAQAAPAKTA
                     PAKQTWEKFKGMIESVNEANKEVVVQAQKEKMSFLVGEHTKISQDSNKMAFSSLKKGM
                     EATVEYKKEGNKMVAEWIDLSPSKAESKPATAPEVKKEMKKENPSEKATE"
BASE COUNT          977 a         1173 c         1288 g          909 t
ORIGIN      
        1 aatttggctg attccagagc cttagaggaa ataaattgca gggataaaga ctaccgggct
       61 gtggcggtgg tggtttatac catggaggaa aaggtggtga tttccgattt taaggaacgc
      121 gggctggatt ttatccttcc cgattccata tgggagggtc tttacaagac tttgtgccga
      181 tagaggatgg gtccgagccg gttccggccg agtcatactt gtttcctgag attctaagtt
      241 cccgggacgg gaggtgggat catgatcagc gatgcttctt cttctttgat gaaagatcaa
      301 ggttcccttc ggtacctcat cgcctttttc tccggcttta cggccgttct tctcttccat
      361 cagggggtcc tttccatcct tcatgccgtg ggcttcaccc cggtcgcacc gtttccgatg
      421 cagccaacaa agccgtttgg gttgccgcaa atctggtccc tggccttctg gggaggggtc
      481 tgggggctcg tgttggcggg ggtggaccgc cggtttccaa ggggagccgg ctactgggtt
      541 gcggcatgca tcttcggggc catcttcccg agcttggttg cctggctggt tgtttttccg
      601 atgaaaggcc tgccagccgg aggggggtgg catgcggccg gattggccac gggcctgatg
      661 atcaatgggg gctggggggt ggggacggct cttctccttc aaggcctttc gaagggatgg
      721 aggtccggag gcccatccat gcggcctgaa tcccctcgaa tatgaagcct tgcccccatt
      781 tgaactgagc cttctccccg ggagaataac gcgagaaatc atcgtatttc atgcctaggg
      841 aggtgaaatc ataatgtcct tgcatagctt gaagaaagtg ctttcggtct tgggtctggc
      901 tccctggttg ctttgcgcat cttctatctg ggcagaaaac ggaggagggg aggaagaagt
      961 tttgcgagcc accctggaaa acggcctccg ggtggttatc gtccgcaata ccctcgcccc
     1021 ggtggtcacg accatcatca actacatggt ggggtcgaac gaggcccccg aggggtttcc
     1081 cggaatggcc cacgcccaag agcacatgat gttccggggc agcccgggcc tgtcggcgaa
     1141 tcagctggcg ggcattatcg cggtcctggg cggaaggttc aatgccgaca cgcagcagac
     1201 ggtcactcag tatttcctta ccgctccggc ggaagacctg gatgttgccc tgcacatcga
     1261 ggcggtccgc atgcgcgggg ttctcgacag cgaaaggctg tgggcgcagg aaaggggggc
     1321 gattgagcag gaagtggccc aggacctatc caatcccgag tacgtctttt acaccaagct
     1381 cctggctcac atgttcaagg ggacgcctta tgcccacact cccttgggaa ccgtgtcctc
     1441 cttcaatcaa acgacgggcg ccatgctcaa gcaattctat gacacctggt atgcgcccaa
     1501 caacgccctc ctggtgatcg tgggagatgt gcagactcgt aaggccctgg aggaggtcaa
     1561 gaagttgttt gccggcatcc ccgccaagaa gattccggag cgacccccca ttcaattgga
     1621 gccggtgacg gcggaggctt ttccgctaaa gaccgatttg tcctacgggt tagtcatgat
     1681 ctcttttcgc ttcccgggct acgatagcga ggactacccc gcgacccagg tcctggcgga
     1741 tgtgttgagc aaccagcgcg gcaatctctt tgccctggtg ccggaaggga aggccctgta
     1801 cgccgggttc accttgagcc ccctgccgaa agctgggctg ggctacgcgg tggcggcgtt
     1861 tcccaaggga gcagacccgt cttccctggt taaggaagtc cggagggtcc tggaggaaga
     1921 tgcgcaaaag ggattccccg cagacctggt ggaagcagcc aaacggaaca aattgaccca
     1981 agcggaactg aagaagaatt cggtgttcgg cctggccatg gcctggtctc aggcgctggc
     2041 ggtggagggg caatcctctc ccgaggaggg ggtcaaggct atacaaaagg ttacggtatc
     2101 cgacgtaaac cgagtggccc ggaaatacct gaacctggat caagccgtcg tggccgtcct
     2161 gaccccggag ccttccggaa aaccagtccc tgctcaggcg caccgagcag tggaatcatt
     2221 cacccccaag gcgaccggcc ctgccaagct gcccgaatgg gcggaaaaat cgctgggccg
     2281 tatttccgtg cccgcttcaa gggtgaaccc ccagatcacc gtccttccca acgggctgaa
     2341 actcatggtg cagcccgagt ccgtcagcaa cacagtgagt gtttacggct atgtcaagaa
     2401 tacccccgac ctggaagcgc cccaaggaaa ggagggagtg gatcaggtct tgaaccagct
     2461 tttctccttc ggcactactt cattggatcg ccgggctttc caaaaagcac tggatgatat
     2521 tggcgcgaag gcagcagccg gcccggactt cttcgtgcag gctctggcca gtgagtttga
     2581 actgggggtg aggctcctgg cagacaacgt gctccgtcca gccttgcctg aaggggcctt
     2641 caaaacggta cagcgacaag ccgcggcctc ggtggcgggg cttctggaaa gcccggagta
     2701 cctggcagag cgtgctttga aggccgccct cttcccccca aacgacccga ccctgcggca
     2761 ggccacgccg gctaccatct cctctctgac cctccgggac gtgaaggact attatcgccg
     2821 gatctttcgc ccggatttga cctccattgt ggtgatcggg aaggtgacgg ccgaggaggc
     2881 caaggcggtg attgagaaac acttcggctc ctggacggct tccgggccga agcctgagac
     2941 tctcctgccc ccggtcgcgc ccagcaggcc gtcgagcacc gcggtcccgg accagagccg
     3001 ggtccaggac aaggtgacgc tagccgaaac tctgggactg aaccgggcca acccggatta
     3061 ctatgccttg caactgggca accacgtttt aggtggagcg ttttatgcca cccggctgta
     3121 tcagggcttg cgggagaaaa gcgggctggt gtattacgtg ggctcttcct tcgatgtagg
     3181 ccagacgagg gcgatctaca tggtaaatta tgcttgcgac cctcagaatg tgtccagagc
     3241 gcgggatatc gtcgagcaga atctaaaaag aatgcacact gccccggtga caccccaaga
     3301 actacaccaa gcgaaagcat tgctgctgcg ggagattcca ctttcggagg cgagtgtgga
     3361 caatatcgcc caaggtctga tctaccgggc aagtcacgaa ttgcctctcg acgagccgac
     3421 ccgggcggcg caccagtacc taaaacttac ggcagaacat gttcaagcag cctttgccag
     3481 gtgggtccga cccaatgacc tagtacagat aactgagggg cctactccaa agtaagctga
     3541 ggaacgggtg acatttcccc attgaattcg gctgttccat tttgccacag attgtttcca
     3601 aattccccgc cccattttcc gctggttcgt ctttaattcc taaaatctat tgaatttaaa
     3661 tggattgcat ttcttccttc cttggcaagc cttttgcttt gattcaacac aagagggatt
     3721 gccaataaga tccccattga ggggcaagat tatggaacct gggagagagg taagagtgaa
     3781 aatttacgat gatcggtttt gcgcggagaa aaggtagagc atgaagaaga gctccccatg
     3841 ggcaacttaa aggcaacccc cccaaattaa gaaaggaagg taaaggaaaa tgaacaaaag
     3901 attgttgatg agtatagttg ctttggcgat ttcacttgct ttcgtttccg gggggatggc
     3961 ccaacagaag ccgactcctg cgcagccaac gcctgcgcag gcagcccccg cgaagaccgc
     4021 tccggccaaa caaacctggg aaaagttcaa agggatgatt gaaagtgtca acgaggccaa
     4081 caaggaggtt gtggtgcagg ctcagaagga aaagatgagc tttctggtgg gtgagcacac
     4141 caagatctcg caggactcta ataaaatggc tttttccagc ctgaaaaagg ggatggaggc
     4201 caccgtggag tataaaaaag aaggaaacaa gatggttgcc gaatggatcg accttagccc
     4261 atcgaaagct gaatcaaagc cagcaaccgc tcccgaagta aagaaggaaa tgaagaaaga
     4321 aaatccttct gagaaagcaa cggaaaa
//
LOCUS       DASPRR010000181         5182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_359529, whole genome shotgun sequence.
ACCESSION   DASPRR010000181 DASPRR010000000
VERSION     DASPRR010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359529"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VEL68_07075"
     CDS             <1..241
                     /locus_tag="VEL68_07075"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ35761.1"
                     /translation="AMGEPVGGLSPYEAAEKSVQAVKNLLAALQVPYRLSQYGIPKDH
                     LPKLIEGGMKQARLFVPNPRDVIQEDLKSIYEKAF"
     gene            348..1355
                     /locus_tag="VEL68_07080"
     CDS             348..1355
                     /locus_tag="VEL68_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ35762.1"
                     /translation="MRSFFKFNGRRGSIALGAVMLLAAAALFSSAAQGAAKDYPNKAI
                     SLIVPWSAGGSTDLSARTLAPVLSKVLGVPITVINKPGGSGIIGTLEAMKSAPDGYTL
                     LADCGGTSSIQYAWAEKLPYKVEERTYIVRAAYTPEALLVPGVSPWKNMDDLMNAIRA
                     NPSAFRWSLIGGTGVPDVITAQLRAALTSKGLDLSKTRSITYKGTGEVLIAIGGNHVD
                     IGFGGVGAATPLRDAGKLRALAIVDSRRYKGWPDIPSTAEIGFAPVDLIYWVGLSGPP
                     GMPANIIKTLTDAVQSAVSSPEMVPQLDKIGFVPGFQPSDEYKKFVLAEGNGIKGLKL
                     K"
     gene            1455..1883
                     /locus_tag="VEL68_07085"
     CDS             1455..1883
                     /locus_tag="VEL68_07085"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ35763.1"
                     /translation="MGLENIVLMALAIVGIYEGTRLVGVPLLIDDPVGPGWYLLFMSG
                     LLFICACALSVRRARRKTVSPSGGLSFLKGPAGRSFLLLLFYGLAALTTGYFIASVLF
                     FTLGQRVFGERSWVRAAMLGLAMTGSFYLVFSYLGGVPLP"
     gene            1916..3409
                     /locus_tag="VEL68_07090"
     CDS             1916..3409
                     /locus_tag="VEL68_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ35764.1"
                     /translation="MEFGLLAGFWKALSPVNLWYCFLGSLLGTVVGVLPALGPATTIA
                     ILLPVTAYLKPTESIIMLAGIFYGAMYGGSTTSILMNIPGEAASVPTCLDGFQMTKRG
                     RAGEALAIAAIGSFIAGTAGVLLLSFAGPLFAELALAFGPPEYFGLMFFSLTALFSFS
                     GHDLLKGLTAGVVGIILATVGLDPLSGTHRLAFGIPQLLAGLDVIPVLIGLFGVAEVL
                     ASAEEEVSSVYQGKLGRLIPRGEELKKGLWASLRGTGIGLLAGLFPGFMPSVVTFISY
                     DIERRVSKHPEKFGTGMIEGVASPEAANNATCQAGFVPLMALGVPTAPIFAMLLASLM
                     IYGLPPGPMLFKQHGDFAWTVVASMYIGNVMLLILNLPLVGIWARLCLVPYRILGPVI
                     LGVVFIGAYSIRNSMFDVWTAILFGLIGFLMKRRDWPVAPLILGFILGPMLEQHLRAS
                     LQGSGGSLRVFIEQPISAGFIVLGVVLVVMSRRLWSAVGKQEAEGER"
     gene            3573..>5182
                     /locus_tag="VEL68_07095"
     CDS             3573..>5182
                     /locus_tag="VEL68_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35765.1"
                     /translation="MKRVWEARVGKVAMRVFLFCVFLYACGGLSWGAEEKPRYGGTLT
                     YAIGAAPPSFDAHRESTYAVIHPIAPHYSLLVKFDQKNYPKVVGDLAESWTVSADSKT
                     YTFKIHNGVKFHDGSLLTSKDIKASYDRIVFPPTGIVSARKAFYDVVEKIEAPDDHTV
                     VFRLKRPSASFMISLASPWNWIYKADILAKDPRWYEKNVMGSGAFKFVEYVTGSHWVG
                     KKHEDYFVKGRPYLDGYRAVFIRDVGARAAAIRGGRVQAEFRYFSPSIRDDLVRAMGD
                     KVKVQELLGGSVNTVIFNCEAKPFNDPRVRRAMSLALDRWEGFKALERIAEVGTSITG
                     LLSPASEFAMTDAELMQLAGFSKNGEASKKEARRLLKEAGVPEGFSIELLNRQEYEPM
                     SIWLIDQWRQVGLNVTQKVQEVGAYYKALRSGEYKVAIHYISDYISDPDLLFLVWLSS
                     DISPANFGRYIDRTLDDLYQKQSSTMNPVERKKLSRQFQTRVLDEMAYGFPPVGWTKR
                     ITIHSAKVKGWVALPHHFLNVDLVDVWL"
BASE COUNT         1013 a         1470 c         1551 g         1148 t
ORIGIN      
        1 agccatgggg gaacctgtgg gggggctctc cccgtacgaa gccgcggaaa aatcggtgca
       61 ggcggtgaag aatttgctcg ccgccctgca ggtcccgtat cgactttccc agtatgggat
      121 tcccaaggac catttgccta aattgatcga aggggggatg aagcaggcca ggctgtttgt
      181 cccgaacccg cgggatgtga ttcaagagga cctgaagagt atatacgaaa aagccttttg
      241 atcctcgctc tcggggagaa gacttctggg cctgcctccg ggatgatatt cggccctctc
      301 ggaccactga cacgagaaga accatacaaa tgataaggag aacatccatg cggtcctttt
      361 tcaagtttaa tggacgaagg ggctcaatcg ccctgggcgc agttatgctt cttgcggcag
      421 ctgccctctt cagttcagca gcccagggag cggccaagga ttatcccaac aaagcgatca
      481 gcctgattgt gccgtggagc gcgggcggct ccacggacct ttcggccaga accctggctc
      541 ccgtcttgtc caaagttctg ggagtaccga taacggtcat caacaaaccc ggcggaagtg
      601 gaatcatcgg caccctcgaa gcgatgaaat cagcgcccga tggctatacc ctcctggccg
      661 actgcggagg caccagctcc atccagtatg cctgggccga aaagctcccc tacaaggtgg
      721 aagagcggac ctatatcgtg cgggccgcct atacgccgga agccctgttg gttcccggcg
      781 tctccccctg gaaaaacatg gatgacctca tgaatgccat ccgcgcaaat ccctccgcct
      841 tccgctggtc cctgatcggc ggtacggggg ttccggacgt catcaccgcc cagcttcggg
      901 ccgcgttgac ctccaaaggt ttggaccttt ccaaaacccg gtccatcacc tataagggga
      961 ctggggaggt cttgattgcc atcggcggca accatgtgga catcgggttc gggggcgtgg
     1021 gcgcggccac cccgctcagg gacgccggga agctccgcgc cctggccatc gtcgattccc
     1081 gtcgatacaa gggctggccc gacattccgt caacggccga aatcgggttc gccccggtgg
     1141 acctaatcta ttgggtcggg ctgtccggtc ctccgggcat gcccgcaaac atcatcaaga
     1201 ccctgacgga tgccgtccaa tccgcggtct cctctcctga aatggttccc cagctggaca
     1261 aaattggatt cgtcccgggc tttcagcctt ccgatgaata caagaagttt gtccttgccg
     1321 aggggaacgg gatcaagggt ctcaaactca aataatcctt ctggaatgcg cgtataccaa
     1381 ggcgaatcgg aaaatttttt ctaatcgata ggctggtgga aattgcgcag taggcgccta
     1441 aggcggagga agggatgggg ctcgaaaaca tcgtgctcat ggcccttgcc attgtcggga
     1501 tttacgaggg gaccagactg gtgggggttc ctcttctcat cgatgatccg gtggggccgg
     1561 gctggtatct tcttttcatg tctggccttc ttttcatctg tgcctgtgcc ctctcagtaa
     1621 gacgggcgag gcgcaaaacg gtttctccaa gcggtggcct ctccttttta aaggggcctg
     1681 cgggccggtc ctttctgctc cttctctttt atggtttggc cgctttgacc accgggtact
     1741 tcattgccag cgtcttgttc tttacgctgg gccagagggt cttcggggag cgatcctggg
     1801 tccgggctgc aatgctgggc ctggccatga cggggagttt ttacctcgtc ttttcgtatc
     1861 ttgggggggt tcccttaccg tgagtcctgc atgagtttcc catagggaat taggcatgga
     1921 atttggactg ctggccgggt tttggaaggc cttaagcccg gtcaatcttt ggtattgttt
     1981 tctgggatcc ctgctgggga ccgtggtggg cgttcttcct gctctcgggc cggccaccac
     2041 cattgccatc ttgctcccag tgaccgccta cctcaagccc accgaatcca tcatcatgtt
     2101 ggcggggatc ttttacgggg ccatgtacgg agggtccacg accagcatcc tgatgaacat
     2161 tccgggggaa gcggcctccg ttcccacctg tctggacggc tttcagatga ccaagcgggg
     2221 aagagctggg gaagcgttgg ccatagcggc catcgggtcc ttcatcgccg gaaccgccgg
     2281 cgttctcctc ctttcctttg ccggccccct gttcgctgaa ttggccctgg ccttcgggcc
     2341 cccggagtat ttcggcctca tgttcttcag cctgactgct ctcttctcct tctccggcca
     2401 tgaccttctg aaaggcttga ccgccggggt tgtggggatc atcctggcca ccgtcggcct
     2461 ggatccgttg agcggaaccc atcgactggc tttcggaatt ccccagctcc tggccggtct
     2521 cgatgtgatc ccggtgctga tcggcctgtt tggagtcgcc gaggtcctgg ccagcgccga
     2581 agaagaggtc agcagcgtgt accagggcaa gctggggcga ttgatccccc ggggggagga
     2641 actgaagaag ggactgtggg ccagcctgcg ggggacgggg atcggtcttc tggccggcct
     2701 gtttcccggg ttcatgcctt ccgtggtcac tttcatctcc tacgacatcg agaggcgggt
     2761 gtcgaagcac ccggaaaaat tcgggacggg gatgattgag ggagtcgctt ccccggaggc
     2821 cgccaataac gccacctgcc aggccggttt tgtgccgctc atggccctgg gggttcccac
     2881 cgcgcccatc ttcgccatgc tcctggccag cctgatgatc tacggccttc cccccgggcc
     2941 aatgctgttc aagcaacacg gcgacttcgc ctggacagtg gtggccagca tgtacatcgg
     3001 caacgtaatg ctgctgatcc tgaatctccc cctggtgggg atatgggccc gcctctgcct
     3061 ggtcccttat cgcatccttg gccccgtaat cctgggggtg gtcttcatcg gggcctattc
     3121 catccggaac agcatgtttg acgtgtggac cgccatcctt tttggcctca tcggatttct
     3181 aatgaaaagg agggactggc cggtggcccc gctgatccta ggattcatcc tagggcctat
     3241 gttggagcaa cacctccggg cctctctcca gggatcgggc gggtcattga gagtgtttat
     3301 cgaacaaccc atcagtgcag gcttcattgt gctgggagtg gtcttagtgg tgatgagccg
     3361 gaggctgtgg tcggcggtgg ggaaacagga ggccgaaggg gaaagatgaa tgggtgagcg
     3421 ggagtccaga catcgtcccc gcgaaaccgg agactctata agaaacccgg actcccgttt
     3481 tcggggcaat gaagaaaaag ggcataaagg gctttttatc attccatgaa ggtcggttcc
     3541 cgaatgaaat tcccatgaag gagggtgcga tcatgaagag agtctgggaa gcacgcgtgg
     3601 gcaaggtagc gatgcgggta tttctatttt gtgtcttctt atatgcgtgc gggggattgt
     3661 cttggggcgc cgaggaaaag cctcgttacg gaggaacctt gacctacgcc ataggagccg
     3721 cgccgccttc cttcgatgcc caccgcgaat ctacctacgc ggtgatccat cccattgcgc
     3781 cccactatag cctgcttgta aagtttgatc agaagaatta tcccaaggtc gtgggggact
     3841 tggcggagag ctggacggtt tccgcggaca gcaagactta cactttcaaa atccacaacg
     3901 gggtaaagtt ccatgacgga agccttctga cgtcgaaaga tatcaaagcc agctacgatc
     3961 gaatcgtctt tccccccacc ggaattgtaa gtgcccgcaa ggctttctat gatgtggtgg
     4021 agaagatcga ggccccggat gatcacacgg tggtcttccg cctgaagaga ccctccgcgt
     4081 cctttatgat cagcctggct tccccctgga actggattta caaggccgac atcctggcca
     4141 aggaccctcg ctggtatgag aagaacgtca tggggtcggg ggccttcaaa ttcgtggagt
     4201 atgttaccgg ctcccactgg gtggggaaga aacacgaaga ttattttgta aaaggccgcc
     4261 cctacctgga cggataccgg gcggtcttca tccgggacgt cggcgcccgg gccgcggcca
     4321 tccggggcgg gcgggtccag gcggagttcc gttacttttc ccccagcatt cgcgatgacc
     4381 tggtccgggc catgggagac aaggtcaagg ttcaggagct gctcggcggt tcggtgaaca
     4441 cggtcatctt caattgtgaa gcaaagccct ttaatgaccc gcgggtgcgg cgcgccatgt
     4501 ccctggccct ggaccgctgg gaggggttca aggcgctcga acgcatcgcc gaagtgggaa
     4561 caagcattac cggcctcctc agccctgcgt cggaattcgc catgactgat gctgagctga
     4621 tgcagcttgc cggtttttcc aagaacggcg aagcctcgaa gaaagaagcc cgccggctgc
     4681 tcaaggaggc gggggttccc gaaggcttca gcatagaact cctgaaccgg caagagtacg
     4741 agcccatgag catttggctc atcgaccagt ggcggcaggt tggactcaat gttacccaga
     4801 aggtccagga agtgggggcc tactacaagg cactccgatc gggagaatac aaggttgcca
     4861 tccattatat ctccgactac atatccgatc cggaccttct ctttctcgta tggctctcct
     4921 ccgatatcag cccggccaat tttggcaggt acatagatcg gactctggat gatctttacc
     4981 agaagcagag cagcaccatg aacccagtgg aacggaagaa gctatcccgc cagttccaga
     5041 cccgggtcct ggatgagatg gcttatgggt tcccgccggt gggatggacc aagcggatta
     5101 ccatacattc ggccaaggta aagggctggg tcgcactccc ccaccacttt ctcaatgtag
     5161 acctggtgga tgtgtggttg ga
//
LOCUS       DASPRR010000182         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1010933, whole genome shotgun sequence.
ACCESSION   DASPRR010000182 DASPRR010000000
VERSION     DASPRR010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010933"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VEL68_07100"
     CDS             complement(<1..1362)
                     /locus_tag="VEL68_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ35766.1"
                     /translation="MLSKTVKDELKAIVGEGRYFDNKEDLLMYSYDAFMVKAMPEVVL
                     LPVSTEEVSRIMKVASREKIPVTPRGAGTNLTGGSVPTRGGMTLAFTKMNRILDIDKE
                     NRNAVVQPGVINMEFQKELAKKGLYYPPDPGSMAVTTMGGNVAENAGGPRAVKYGVTK
                     DYLLGLEVVLASGEVLRTGGKTLKNVTGYNLTQLFCGSEGTLGLITEMTLKLIPLPET
                     KRTLQAAYKNMSDAGRTISKVFEIGILPVALEILDKTFINIIEDYTHLGLPREAEALL
                     LIEVDGPETAVVPQSERLTKLCQEMGAHEVKVAKTVAENDEIWRARRSAYGAEARLRP
                     TAIAEDCTVPVGNLVAMFHEVAKIAEKYKLLIPIVAHAGDGNLHPQILTDIRDKEEME
                     RVMKAIDEISIKAVELGGTLTGEHGIGIAKREILPLELGEAAMEVTRNIKKAFDPLNI
                     LNPD"
     gene            complement(1365..2726)
                     /locus_tag="VEL68_07105"
     CDS             complement(1365..2726)
                     /locus_tag="VEL68_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ35767.1"
                     /translation="MKATLAIVNGLLVDSTGIYSGVLYLRDGKIVGITQELENQPPKV
                     IDARGLCILPGAIDGHVHMMDPGYTDREDFITGTRAAARGGVTTVIDHHRTVPQVFAA
                     GELIAKKDYLEKRAVVDFGLLGGLSLTNLKALRGMWEAGALGFKGFTCELHEADALEG
                     NRVTDILDEIRRFDGVALFHCEDNVLLKGKERQLRVQGRKDPLTISEWRSPEAEELAV
                     RALINAARKTGARVAVAHTSLPSLVLEQAAARAQGVPIFTETCAQYLYLTEEDLRKQG
                     PFAKFTPPPRKKEDVEKMWWCAAQGLIDMVNSDHCPYPYADKEKGLKDIWQAPFGIPG
                     VETVTPILLDLVSRGLLTLPRVAWLRSERPAMIYGLSGQKGALRVGCDADLVLVDLNK
                     KVKFDNAKVVSKCGWTPYHSREVTGDVVLTMVRGKVVMEEGKVVGEPGWGKFVTRRKD
                     GDQ"
BASE COUNT          570 a          959 c          797 g          730 t
ORIGIN      
        1 atccgggttt aggatattca ggggatcaaa ggcctttttg atattccggg taacctccat
       61 cgccgcttct cccaactcca ggggaaggat ctcccgtttg gcgattccga ttccatgttc
      121 tccggtcagg gtgcccccca gctccaccgc cttgatggaa atctcgtcga tggccttcat
      181 gaccctttcc atctcttctt tatcgcgtat atccgtgagg atctggggat gcaggtttcc
      241 gtcccccgca tgagccacta tggggataag aagcttatac ttctccgcga tcttggccac
      301 ctcatgaaac atggccacca aattccccac gggcaccgtg cagtcctcgg caatggccgt
      361 gggcctcagc cgtgcctccg ccccgtaagc ggaacgtctg gccctccaga tctcatcgtt
      421 ctcggccacg gtcttggcca ctttgacctc atgggccccc atctcctggc acagtttggt
      481 gagccgctcc gactggggaa ccactgctgt ttccggaccg tccacttcga tgagcagcag
      541 ggcctccgcc tcccggggca gccccagatg ggtgtaatcc tcgatgatgt tgatgaaagt
      601 cttgtccaga atctccaaag ccactggaag aatcccgatt tcgaagactt tggagatggt
      661 cctacccgcg tcgctcatgt tcttgtaagc tgcctgaagc gtcctttttg tttccggcag
      721 ggggatcagc ttcaaggtca tctcggtgat caaccccagg gttccctccg aaccgcagaa
      781 cagctgggtc aggttgtatc cggtcacgtt cttgagagtc ttccctccag tgcgcaagac
      841 ctcgccggaa gcgaggacca cctccagccc gaggagatag tctttggtga ctccgtattt
      901 aaccgccctc ggccctcccg cattctcggc cacgtttcct cccatggtcg tgactgccat
      961 ggacccgggg tccggcgggt aatataatcc ctttttcgca agttctttct gaaactccat
     1021 gttgatcacc ccgggctgaa cgactgcgtt ccggttttcc ttatcgatgt cgaggatccg
     1081 gttcatcttc gtgaaagcca gggtcatccc tccccgggtg ggcacggacc ccccggttag
     1141 gttggttccc gctccccggg gggtcaccgg gatcttttcc cgggaggcca ccttcatgat
     1201 ccgggagacc tcttcggtgc tcaccggcaa aagcacgact tcgggcattg ccttgaccat
     1261 aaaggcgtcg taggaataca tgagaaggtc ttccttgttg tcgaaatacc ttccttcccc
     1321 cacgatggct ttaagctcgt ctttgactgt cttgcttaac atggttactg atctccatct
     1381 ttccttcggg tcacgaattt cccccagccg ggctctccaa cgacttttcc ctcctccatg
     1441 accacctttc cccgcaccat ggtcaggact acgtccccgg tcacttctct gctgtgatac
     1501 ggtgtccagc cgcacttgga aacgactttc gcattatcga actttacttt cttattcagg
     1561 tctaccagaa ccagatcggc gtcgcaaccc acccggagcg cccccttctg cccgctcaga
     1621 ccataaatca tcgccggtct ttcgctccgc agccaggcga cgcgcgggag cgtgaggaga
     1681 ccccgggaga ccaagtcgag gagaatcgga gtcacggtct cgactccggg gatcccgaag
     1741 ggagcctgcc agatgtcctt caagcctttt tccttgtcgg catagggata ggggcagtga
     1801 tccgagttca ccatgtcgat gagcccctgg gcggcgcacc accacatctt ctccacgtcc
     1861 tccttcttcc tcggcggggg agtgaacttg gcaaaaggcc cttgcttcct caagtcctcc
     1921 tcggtcaggt aaagatactg ggcgcaggtc tcggtaaaaa tgggaactcc ctgggctcgg
     1981 gctgcggctt gttcgagcac cagggaaggc aggctggtgt gggccacggc gacgcgcgct
     2041 cccgtcttcc gggcggcatt gatcaaagcc cggacggcca gctcctccgc ctccggagac
     2101 cgccactcgg aaatggtcaa gggatccttg cggccctgca cgcgcagctg tctctctttg
     2161 cccttcagga gcacattgtc ctcgcagtgg aagagggcga caccgtcgaa gcggcggatt
     2221 tcgtcgagaa tgtccgtcac tcgattgccc tccagagcat ccgcttcgtg caactcacag
     2281 gtgaacccct tgaaccccag ggcccccgcc tcccacatcc cccggagagc tttcaaattt
     2341 gtgaggctca gtccgcctag gaggccgaag tctaccactg cccgcttttc caaatagtcc
     2401 ttcttggcga tcaactcccc ggccgcgaaa acctggggta cggttcggtg atggtcgatc
     2461 accgtggtga ccccgcccct ggcagccgcc cgggttccgg ttatgaaatc ttcgcggtcc
     2521 gtatagcctg ggtccatcat atggacatgt ccgtcgatgg ccccgggaag gatacagagg
     2581 ccccgggcat cgatcacctt tgggggttga ttttccaact cctgggtgat ccctacgatc
     2641 tttccatcgc ggaggtagag cacccccgag tagatgcccg tagagtcgac cagaagcccg
     2701 ttgacgatgg ccaaagttgc tttcatagaa ctacctcttt ttgtatgagt ttagctatct
     2761 gaaatggtct tcttaaatcc cccctccgcc cccctttttc aaagggggga acggggggat
     2821 ttctgcagat gctttgctca aaaaggagat gtagaaaaat atgcctgttt tttttaaaaa
     2881 accaaagtgt tacctttttt agccgtacgg gaacgatgaa ttttgcgcct accttccggc
     2941 gttgaaggtg ggcaggcggc acgatcaaac gcccagatac cgttctttca cctcccgatt
     3001 ttcccacagc tccgccgggg ttgaggagtg gacgatcttc cctttgctca tcacat
//
LOCUS       DASPRR010000183         4165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_200333, whole genome shotgun sequence.
ACCESSION   DASPRR010000183 DASPRR010000000
VERSION     DASPRR010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200333"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..339)
                     /locus_tag="VEL68_07110"
     CDS             complement(70..339)
                     /locus_tag="VEL68_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXZ35768.1"
                     /translation="MKVEDKLALNKFEIDKEVHIQVEEKLCPDCDLKPCLYVCPAECY
                     QLRENHVTFSYEGCLECGSCRLACDRQAIRWVYPRGGFGICYQYG"
     gene            complement(336..1622)
                     /locus_tag="VEL68_07115"
     CDS             complement(336..1622)
                     /locus_tag="VEL68_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ35769.1"
                     /translation="MMDKFDAVVVGAGPAGCAAAYTLAKAGLQVLVCERGKYAGAKNM
                     WGGAFFGPQMFELFPDFAKEAPVERFVNRHTITLLDQEDALTFDFRTPHSPGSHHGFI
                     TLRAKFDRWMAEKVEQAGAIVATSLQADDLVREGNFIKGVKAGTEEFPADVVILAEGV
                     NSLLTEKGGLRKPDSAHDMKQGVKEVIRLPKEILEERFQLQGDEGVAMEFLGSCTQGL
                     PGGGFLYTNRDSISLGLVVQLSAVLEKRVKISELLEDFKKHPIIQPLIRKGEMVEYSA
                     HLIPVSGLKMMPRLFSDGILVVGDAAALVLGTGLILEGANFSVASGIAAAKTVIHAKE
                     KGDFSQAALQHYQKLLQDEETLRDFETFRRAPEFLENERIYAVYPQWACGLARKIFTN
                     DGKPRRNTFQVLRESMKGKVTLGNLISDAMKARKAL"
     gene            complement(1850..3007)
                     /locus_tag="VEL68_07120"
     CDS             complement(1850..3007)
                     /locus_tag="VEL68_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35770.1"
                     /translation="MDFDLTQEQKMFQDMAKEFAQREIAPIARDLDRNEKFPHDLIPK
                     MGEQGLFAIKIPNEYGGIDLDWTTLGLVAEQFASVDFSTALTYYIQTSLEAMPILTAG
                     TEEQKKKYVPALLQGKIMGCTAAVEPNVGSDATAVETNAVRDGDWWVLNGNKTWITNA
                     TVADFAVVVAQTDKSKGHRGIATFVVDKGTPGFSSTKITNKLGCRSTDTGQLFFRDCR
                     IPRSNELGPVGKGMSTALTCIEHTRFGIAWMGLGLTRGCLEASIKYCQERTQFGKPIG
                     SFQLIQERIADMVVDLEASRLLIYQAAHLKDKGQPSTREVSIAKYHNIEVAVRATKTA
                     VELHGAYGYTDDFPVERAYRDIIGPLIFGGTANVQRLILGRMTLGIDAISR"
     gene            complement(4015..>4165)
                     /locus_tag="VEL68_07125"
     CDS             complement(4015..>4165)
                     /locus_tag="VEL68_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35771.1"
                     /translation="EQNATMALHVSHRGYVMETGRIVLQGPAKDLLQNPQVRDAYLGG
                     SKGPS"
BASE COUNT          814 a         1247 c         1200 g          904 t
ORIGIN      
        1 aatttggccg ggggcaggtc cgggtccaga acgggtttca cgcaaacgat aatctccatc
       61 tctcctcctt caaccgtatt gatagcagat gccgaatcct ccccgggggt acacccaccg
      121 gatggcctgc cggtcgcacg ccaggcggca gctgccgcat tccaggcagc cttcgtagga
      181 gaaggtaaca tggttctccc gtagctggta gcattcggcc ggacagacgt agaggcacgg
      241 cttcaggtcg caatccgggc agagtttttc ctccacctgg atgtgaactt ccttgtcgat
      301 ctcgaacttg ttgagcgcca gtttgtcttc gactttcaaa gggccttcct cgctttcatg
      361 gcgtcagaga ttaaattccc gagagtcact ttccccttca tgctttcccg caggacctgg
      421 aatgtattcc ggcgcggctt gccgtcgttg gtgaagatct tcctagccag cccgcaggcc
      481 cactgcgggt agacggcgta gattctctcg ttctccagga attcgggggc ccggcggaag
      541 gtttcgaaat cccgcagagt ctcttcatcc tgtaaaagct tttggtagtg ctgcagtgcg
      601 gcttgcgaga aatccccctt ctctttggcg tggatgacgg tcttggccgc ggcgatgccg
      661 gaagcgacgg agaagttggc cccctccagg atcagaccgg tccccaggac caaggcagcc
      721 gcatccccca cgactaggat gccgtcggaa aagaggcggg gcatcatctt caggccggat
      781 acgggaataa ggtgggccga atactccacc atctcgccct tgcggatgag gggctggatg
      841 atgggatgct tcttgaagtc ttccagcagt tcggaaatct tcacccgctt ctccaggacg
      901 gcgctcagct gcaccactag gcccagggag atgctgtccc ggttggtgta aaggaaccct
      961 cccccgggga gcccctgagt gcaggagccc agaaactcca tggccactcc ttcgtcgccc
     1021 tggagctgga agcgctcttc cagtatttcc ttgggcaggc ggatgacttc tttaaccccc
     1081 tgtttcatgt cgtgggccga gtcgggcttg cggagtccgc ctttttcggt cagcagggag
     1141 ttgaccccct cggccaggat gaccacgtcc gcgggaaatt cctcggtccc cgccttcacc
     1201 cccttgatga aattgccctc ccggaccagg tcgtcggcct gcaggctggt ggccacgatg
     1261 gccccggcct gctccacctt ttcggccatc cagcggtcga atttggctcg cagggtaatg
     1321 aacccatggt gagaaccggg agaatgggga gtccggaaat caaaagtcag ggcgtcttcc
     1381 tggtcgagga gggtaatggt gtggcggttc acgaacctct cgaccggagc ctctttcgcg
     1441 aaatcgggaa agagctcgaa catctgggga ccgaagaagg cgccccccca catgttcttg
     1501 gcgccggcat actttccccg ctcgcagacc aaaacctgca gaccggcttt ggccagcgta
     1561 taagcggcgg cacatccggc cggaccggcc ccgaccacaa ccgcgtcaaa tttatccatc
     1621 atgaaagcct ttccatcctt atttcaaaac atcctgatca acggaagcat gccacccttg
     1681 ggcaaagatc cctgataagc cgacccatcg ctgaaatccc cccccaaccc ccctttaaca
     1741 aaggggggca ggggggattt ctggcaggcc cgtcgcgttg atgaaaatcc tatctccctg
     1801 gtgcggctct ccttgcaccg gaccatccca agggatcagg cctcggagtt cagcgggaga
     1861 ttgcgtcaat gcccagggtc atccgtccaa ggatgagtct ctggacattg gccgttcccc
     1921 cgaagatcaa cgggccgatg atgtcgcggt aagcccgctc caccggaaaa tcgtcggtgt
     1981 acccgtacgc cccgtgaagc tccacggccg tcttggtggc gcgcaccgcc acttcgatgt
     2041 tgtggtattt ggcgatggat acctcccgcg tgctcggctg acccttgtcc ttgagatggg
     2101 ccgcctggta gatcagcagc cgggatgcct cgaggtccac gaccatatcg gcgatccgct
     2161 cctgaatcaa ctggaagctg ccgatgggct tcccgaattg ggtgcgttcc tggcagtact
     2221 ttatgctcgc ctccaggcag ccccgggtca gacccaatcc catccaggcg atgccgaagc
     2281 gggtgtgctc gatgcaggtg agtgccgtgg acattccctt gcccaccggg ccgagctcgt
     2341 tggaccgggg aatgcggcaa tcccggaaga agagctggcc cgtgtccgtg gaccggcagc
     2401 ccagcttgtt ggtgatcttg gtgctggaaa aacccggggt ccccttgtcc acgacaaagg
     2461 tggcgattcc ccggtgtcct ttgctcttgt ccgtctgggc cacgacgacg gcgaagtcgg
     2521 ccacggtggc gttggtgatc caggtcttat ttccgttcag gacccaccaa tccccgtccc
     2581 ggacggcgtt ggtctccacg gccgtggcgt cgctgcccac gttgggctcc acagccgccg
     2641 tgcagcccat gatctttccc tggagaaggg cggggacgta cttcttcttc tgctcctcgg
     2701 tgccggcggt caggatgggc atggcttcca gggaagtctg gatgtagtag gtcagagccg
     2761 tggaaaaatc cacgctggcg aactgctcgg ccaccagtcc cagggtggtc cagtccaagt
     2821 ctatgccccc atactcgttc ggaatcttga tggcgaataa cccctgctcc cccatcttgg
     2881 ggatgaggtc gtgggggaat ttttcgttgc ggtccaagtc cctggcgatg ggagcaattt
     2941 cccgctgcgc aaactccttg gccatatcct gaaacatctt ttgttcttgg gttaaatcga
     3001 agtccatttt cttcctccag ttagggtaag agcttggaaa ttcgctcaga atgacccggt
     3061 tcttcctccc cccaggggag gagatcggag ttctgttttc cggtcgggac ttcggcgccg
     3121 acgaagagaa gtagcgcccc cagccggtta gcaaaagcgg gtcggccccg ccgatctttg
     3181 ggggttaggc aaccaaaggc gaaaggagga ccttggggga aaatcattca ggcttaggcc
     3241 tgaaggagca aatgccccac gccccagcct gactgttctt agccggaact gattttgccg
     3301 gaaggagctc gtgtgaagtt cggtccgatg gaatcgatgg ccggcgcaaa aaaagtgaat
     3361 ctcggaaagg tgatcctcga agaggatgag aaccgtgcgg gtaattttcg ctagtctccc
     3421 ggtagaatgt ccctccctcg aagatccccg gtctcacttt aattttctcc ccgtggcttc
     3481 tcacggggaa tgaaagcaat tccccccttt ggttcgcttc cccgccgagg tgaacacggt
     3541 tccaactccg ctgaatacct tgccccccgg tcttgttacc gcaaatcagg atatccaact
     3601 gttagcacaa atggagataa aagatcaaat ctttttttcc ccggatgggc tctcccctga
     3661 agggaaaggg caacctcggg ttagcccccg ggtttttccc tttgcttttc aatatccgac
     3721 tccggcaggg ccgatacctt agaagataga ttcatcgttc catgtaagcg ggacccgccg
     3781 ggtctcccct gcaataaagg ccatgatcga acttacgtcc ccaactctgg cccgatttca
     3841 gtcgtaagag aataaaggat gtctgcccag aagtagagtg ggaagaggcg aaactccggt
     3901 ttgttttccg cgaaagagtg attttttgaa attcttgggg gggcagcggg caggatctcg
     3961 accaccctca gagcctaaaa aaggccgcga ggaaacttga ccgccagcgt acggtcacga
     4021 tggtccctta ctgccgccga ggtaggcatc ccgaacctgg ggattctgca ggaggtcttt
     4081 ggccggcccc tggaggacga tgcgccccgt ctccatcacg tatccccgat gagagacatg
     4141 gagcgccatg gtggcgttct gctcc
//
LOCUS       DASPRR010000184         9462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_532741, whole genome shotgun sequence.
ACCESSION   DASPRR010000184 DASPRR010000000
VERSION     DASPRR010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532741"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VEL68_07130"
     CDS             <1..585
                     /locus_tag="VEL68_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35772.1"
                     /translation="QTINGRKVSLFNFAGTGTTSANDAEPANYQVSTGALSLSGISAG
                     TPVRVRGLVRAFGQAPPDFTAWTIINVAALPATLFFDWNPASAAPFSSISPTGMTLNP
                     AGVGTLHHILRGWVWTDVLPNSPIVQPKSAPLGLYAICYQGTVQLYTQFDAYSQALQT
                     SLNSGNKARLLCAYGTFTDATTTLDATRVLAAFQ"
     gene            complement(821..1561)
                     /locus_tag="VEL68_07135"
     CDS             complement(821..1561)
                     /locus_tag="VEL68_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXZ35773.1"
                     /translation="MGELVLRPLHVGTIRRDKSIFTYLRNMGTQIEMPILVWYIEGQG
                     HRILVDTGGHDPAKGELHPPYMREPGQDPVSRLAALGVRPEDIDILILTHLHWDHASN
                     VDLFPKARVILQREELRYAAAPLDPHRWAYQSIPPFLQFLERIDLIEGDKEIARGVSV
                     HLTPGHTPGIQGVAVRVPSGRYFIAGDNVPLMENWEAKGKYGVPHWPNSIHVDLEIFL
                     KTLRRIEDLGDIILPGHDWSALEKTEYK"
     gene            complement(1609..2691)
                     /locus_tag="VEL68_07140"
     CDS             complement(1609..2691)
                     /locus_tag="VEL68_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ35774.1"
                     /translation="MMEKGKSICRPDRFGEKGERPSNPSRLSIRWFLKAASLAVVGLG
                     MGGTTLLTPQATSAQPQVLEYKFADVQPAQGIYGRYEVKFGKLMSERTGGAVKIAHFG
                     GGSLGGEKDVMEGLRLGSVHISLIGLTLMPFLDVIWGPYVFRDNDHGRKVLDGEIGES
                     WKQRVLKEGGGLRLIDYVYFGPRQLTTKNTPGRTPKELKGLKIRVMEAPIYIATWKAL
                     GASPVPMAWPEVFTGLQQGTIDAQENPLELIVSNSLFEVQKYVALTSHVRPYRFLLMN
                     DAAFNKMSKEHQKVFVDTWKEIAKEIEQEYISSDQKYLDLLKSKGMTVVQADVNAYRE
                     ATKDVWKQFMPKAWGEGVYEKVQAVK"
     gene            complement(2801..4096)
                     /locus_tag="VEL68_07145"
     CDS             complement(2801..4096)
                     /locus_tag="VEL68_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ35775.1"
                     /translation="MSLFLLFALMLGFMALGMSIAVAMIASSLIYLLINASGPMSLPL
                     AVIPQKLLYGVDSFPLLAIPLFMLAGELMSAGGITNRIVRFATALVGWITGGLAHVTI
                     VVNMIMAGMSGSAVADAAATGSVLIPAMIKARFPAAFAAAVTAAAATIGPIIPPSIPF
                     VLIGTIAQISIGRLFLGGAIPGIIMGLFLMAVSYWVAKRHGYAVEKRATFREIVVSFW
                     DSAPALIMPLIIMGGILSGIATPTEAAVLAVAYALFLSVVVYRKLSLSEVYRICWRMG
                     SATAAIMITISGATLLGFVATAEQMGPKLIQLFLSLSDNPHLLLLIINIALLFLGWVM
                     EPVPIILLTVSVFFPVLTKIGMDPIHLGVVMTLNLMIGLLTPFVGLNVFVTAAISNVP
                     VIKVARESLIFIATLITVLFLISYVPELVLWLPNTVMGK"
     gene            complement(4093..4542)
                     /locus_tag="VEL68_07150"
     CDS             complement(4093..4542)
                     /locus_tag="VEL68_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ35776.1"
                     /translation="MIAMVVLTLLQVTSRYALQSPYPWTEELARIDLIYLTFFGSIVA
                     FQRREHLKIDILVHALSQGSRRWLRVIVDGASMLVLGVVVWQGAPLLHKFWPMLSAAL
                     NWPTTVFYFPVVFGCLVMLIYTIQDLVAAIRNTDSERRIGSAGEEST"
     gene            complement(5253..7115)
                     /locus_tag="VEL68_07155"
     CDS             complement(5253..7115)
                     /locus_tag="VEL68_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009367327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HXZ35777.1"
                     /translation="MPQSYEGSHQQYDALIDSSAMIPMRDGVKLATDLYFPASGDRQA
                     AGKFPVILERTPYGKGSSGSVLKGKYFARRGYVCAIQDVRGRFDSEGDWYAFAKEAHD
                     GYDTVEWLGAQKWSAGKVGTMGDSYAGSDQSALATLSPPHLSTMIVAVGASNYYHSSM
                     RHNGALELRFHVYVFRMAITSKEAAADPNLKKALIKAYTEEMPEILRRSPLKKGATLL
                     RRLPSYEQWALDILTHGDYDSYWKEHRGYAISEYYREHADVPTLYLGGWYDSYARNTC
                     ESFVRLRKIKKSPQYLLMGPWTHGKYEITYSGDLDFGTEAEINYLDLKLAWFDRFLKG
                     MNTEVADWRPVQIFTMGTGGGRRVIDGAPGEASDYPGRIHHGGFWRGMDDWPIPGTRF
                     TPYYLQADGNLSAEKPAKPDIPPSRYTFNPRDPVPTLGGGISAVDIVMRPGAFDQRGR
                     SGSIGCTDKQPLSLRSDILTFQTPPLESDVEITGPIEMHLWASSSAADTDFTAKLIDV
                     YPPDEDEPDGLAINLTDSILRARYRNGFEKPEFLEPGKAYEFVFQLYPTSNIFKKGHR
                     IRLDISSSNWPRFDVNPNTGGPLGLEQRYNLAHQAVYHDAAYPSHIVLPLQKGG"
     gene            complement(7384..>9462)
                     /locus_tag="VEL68_07160"
     CDS             complement(7384..>9462)
                     /locus_tag="VEL68_07160"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXZ35778.1"
                     /translation="VEGDKVMRRFQIGATVENDYQSVDAPTRAMLDAYAAGVNAFIES
                     TDALPIEYKLLDARPEPWHPRDSFAVFKVRHIMMGVFEGKLWRAKLVNTLGTEKAAAL
                     LGGHPPGDLVIAPPGGTYKGPVLNGLEELSRNLEGIRWLQDEPDSGSNNWAVHGSRTS
                     SGKPFLAGDAHRLLDTPNCYYQNHIACKEFDVMGISFPGVPGFAHFGHNPHVAWCVTH
                     AQADYQDLYIERFSPEGASRYEFRGQWKEAEVRRERIKVKDETDQALEVTLTRHGPII
                     AGGPGKGYGLAFQYTATAFPYRGFECLLPMMKAASVDELNESMRKWVDPCNNLVSADV
                     HGNIAYLHRGQVPIRSMSNAWMPVPGWSGDHEWQGSIPFEKLSRLRNPSTGFIVSANN
                     RIPDQKYPYYIALSYAPEYRARRIYERLENLTGATMEDMRSIHGDSISIPAQVFIKLI
                     AEAKLTNEFSIKAQSMLVRWNGAMERDAVAPTIYSAFRVKLLHKIIGSRMGPLVDVMF
                     NSTGRGAPRHLGELASQIVHQAKTGDAAFLPQGVTWGSIAGEALNEAVAYLRQRLGGD
                     METWKWNTIHRTSPKHPISHLFPGLENFLNPPPVSMGGDGDTPYAAGYSPGRPFDITL
                     LSVVRYIFDISDWDNSGWAVPLGVSGHPGSRHYADQASIWGNLELIPMRYTWDRVKAE
                     AAVPQILEPR"
BASE COUNT         1973 a         2712 c         2792 g         1985 t
ORIGIN      
        1 cagacgatca acggccggaa ggtgtcgctc tttaactttg cgggaactgg aacgacatcc
       61 gccaatgatg cggagccggc caattatcag gtgagcacgg gagctttgag cttaagcggt
      121 atctctgcgg ggacgccggt ccgggtaaga gggctggttc gggctttcgg ccaggcgccg
      181 ccggatttca ccgcttggac cataatcaat gttgcggccc ttccggcgac tctcttcttc
      241 gattggaacc cagccagtgc agctcctttc tcaagcatct cccccaccgg catgactctc
      301 aatccagcgg gggtgggtac gctccaccac atcctgcgcg gttgggtgtg gacggatgtt
      361 ctgccgaact cccccattgt acagccaaag agcgctccct tgggcttgta tgcgatctgc
      421 taccagggca cggtccagct gtacacccag tttgatgcct attctcaggc tctccaaacc
      481 agtctgaatt ccgggaacaa ggcccgcctg ctatgtgctt acggaacctt caccgatgcc
      541 acaaccaccc tcgacgcaac ccgggtcctt gccgccttcc aataaattca tttgggtaaa
      601 cccccggctc tgccggggga cacccaaaat ctgaccttat ccggggtggt taggttgtgg
      661 tcaccagtcc aaggccacct ttaggtgggg accatttcaa tccgctttga gcgattcact
      721 tttttggggc tttctggtca aaaaaggtca cggttgggat gccgattctt aagcacccct
      781 accgtggtcc tgggatgtgt cgcatttttc tgaaggatgg ttacttgtat tcagtctttt
      841 ccagagcgct ccagtcgtga ccggggagga tgatgtctcc caaatcttct atccggcgca
      901 gggttttcaa gaagatttcc aggtccacgt ggatgctgtt cggccagtgc ggcactccat
      961 attttccctt agcttcccaa ttttccatca gagggacatt gtcgccagca atgaaatacc
     1021 ttccgctggg tacccgcacc gccaccccct gaatcccggg ggtatggccc ggggtgaggt
     1081 ggacggagac gcctctcgcg atttccttgt ccccttcgat caggtcgatg cgctccaaga
     1141 actggagaaa gggggggata gactgataag cccaacgatg agggtctaag ggggcggcgg
     1201 cgtagcgcaa ctcttcccgc tggaggatca cccgggcttt cggaaaaagg tcgacgttgg
     1261 aagcgtggtc ccaatgcaga tgggtgagga tcagaatatc gatgtcctcc ggccgaacac
     1321 ccagggcggc gagccgggat acagggtcct ggccgggctc ccgcatgtat ggggggtgca
     1381 attcgccctt ggccgggtcg tgcccgcccg tgtccaccag aatccggtgc ccttggccct
     1441 cgatatacca gaccaggata ggcatctcga tctgggtccc catgttcctg aggtaggtaa
     1501 agatcgactt gtcccgccga atcgttccca cgtgaagggg tcttagaacc agttctccca
     1561 tcgcagttcc tcccaaagca aatccattcc cgttccggaa tgatttttct acttcaccgc
     1621 ctgaaccttc tcgtagacgc cttctcccca ggccttgggc atgaactgct tccagacatc
     1681 cttagtggcc tcgcgatagg cattcacgtc ggcctggaca acggtcatgc ctttgctctt
     1741 cagaaggtcc agatacttct ggtcgctgga tatgtattcc tgctcaatct ccttggcgat
     1801 ttccttccag gtgtccacga agaccttctg atgctctttg gacatcttgt tgaaggcggc
     1861 gtcgttcatc agcaagaatc ggtaggggcg cacgtgagag gtcagggcaa catatttctg
     1921 gacctcaaac aggctattgg agacgatcag ctccagggga ttctcctggg cgtcgatggt
     1981 gccctgctgc aatccggtga aaacctcggg ccaagccatg gggactgggc tggcccccaa
     2041 ggccttccag gtggcgatgt agatgggagc ttccataact cggatcttca agcccttcaa
     2101 ttccttgggg gtcctgcccg gagtgttctt ggtggtcaat tggcgagggc cgaaatagac
     2161 atagtcgatc agcctcagcc cgccgccctc cttgagaact cgctgcttcc agctctcccc
     2221 gatctctccg tcgagtacct tccggccgtg gtcgttgtcc cggaagacgt agggacccca
     2281 gatgacatcc aggaagggca tcagagtgag tccaatcaga ctgatgtgca cggaccccag
     2341 ccgcaggcct tccatgacgt ctttctcgcc ccccagggaa ccgcccccga agtgggcgat
     2401 cttcaccgcc ccgcctgtgc gttcggacat gagcttgccg aacttgacct cgtaacggcc
     2461 gtagatgccc tgggctggct ggacatcggc gaacttgtat tccagcacct ggggctgggc
     2521 ggaagttgcc tggggggtca acagggtggt cccccccatc ccaaggccca ccactgccag
     2581 agaggcagcc ttgaggaacc agcggatgct caaccggctg ggattcgagg gcctctctcc
     2641 cttctccccg aagcgatccg ggcgacaaat actctttccc ttttccatca tttttatccc
     2701 tccttatgga attttgagct gggaaaaacc ctagaccttg atctctctaa ttcccttctt
     2761 tcgggcgatc tcccaactct cagcctccag gacttgatat ttacttgccc ataacggtgt
     2821 tgggaagcca gagcaccagc tcgggcacgt aactgatcag gaataacacc gtgatcaacg
     2881 tggcgatgaa gatcaatgac tcccgggcca ccttgatgac cgggacgttg gaaatggccg
     2941 cggtgacgaa aacgttgagc cccacgaagg gcgtgagcag gccgatcatt agattcaggg
     3001 tcatcaccac ccccagatgg atggggtcca ttccgatttt ggtgaggacc gggaagaata
     3061 ccgacacggt cagaaggatg atgggcacag gttccatcac ccatcccaag aatagcaggg
     3121 caatattgat gatcagaagg agaaggtggg gattgtcgct caaggaaagg aaaagttgga
     3181 tcaacttggg acccatctgc tccgcagtgg cgacaaagcc gagcagggtg gccccggaga
     3241 tcgtaatcat aatggctgca gtcgcacttc ccatccgcca gcagatccgg tagacctcgg
     3301 atagggacag cttccggtaa acgacgacgg aaagaaaaag ggcgtaggcc accgcgagaa
     3361 ccgcggcctc cgtgggagtg gcgatcccgc ttagaatccc gcccatgatg atgagcggca
     3421 tgatcagggc gggggccgaa tcccagaaac ttaccacgat ctcccgaaag gtagcccttt
     3481 tctccacggc atagccgtgg cgtttggcca cccagtagga tacggccatc aggaagaggc
     3541 ccatgatgat gcccgggata gcgcctccca ggaatagcct tccgatggat atctgggcga
     3601 tcgttccaat cagcacgaag ggaatgctgg gtggtatgat ggggcctatg gtggccgcag
     3661 ccgcagtgac cgccgccgcg aaggcggcgg ggaaccgggc cttgatcatg gctgggatca
     3721 agacggaacc ggtggcggcg gcatctgcca cggcggaccc ggacatgccg gccataatca
     3781 tgttcacgac gatggtgaca tgcgccagcc ctcctgtgat ccaccctacc agggcagtag
     3841 caaaccgaac gatcctattc gtgatccccc cggcggacat cagttcgccg gcaagcatga
     3901 agagggggat agccaggagg gggaaagagt cgactccgta aagcagcttc tgaggaatta
     3961 ctgccagggg aagcgacatg gggccggaag cgttgatcaa taggtagatc agactgctgg
     4021 cgatcatggc cacggcaatg gacatgccca gggccataaa gcccagcatc agggcgaaga
     4081 gcagaaaaag gctcacgtgg attcctcccc ggcggaaccg atcctccgct ccgaatcggt
     4141 attccggatc gctgccacga ggtcctggat ggtatagatc aacatgacga ggcagccgaa
     4201 gaccactgga aagtaaaata ccgttgtggg ccaattcagg gctgcggaga gcatgggcca
     4261 gaacttgtgc agcaggggag cgccctgcca aaccaccaca cccaacacta gcatcgaggc
     4321 cccatcaacg atcacccgaa gccaccttct tgatccctgg gataaggcat gcaccaggat
     4381 atcaattttg aggtgctcac ggcgctggaa ggcaacgatg gacccgaaga aggtaagata
     4441 aatgaggtcg attcgggcta gctcttctgt ccaagggtag ggggattgaa gggcatagcg
     4501 gctggtgact tgcagcaacg tcaggaccac catcgcgatc atgaggaagg cggtgatggc
     4561 ctggaatccg agatcgatcc ccttcttgaa actcagagat ttcccctttc tacctatgtg
     4621 cctgattcat tgaccccggg gtgatgggcc gcagaccatg cgcctccccg atcaatagcc
     4681 gctcttaaat aggaggagta taggagaaaa cggatggagg atcaacagaa aaaatccatt
     4741 caagggccaa agtggaatca caggacgatc ctgccctgtt tcccaattct ctgacccttc
     4801 ggcgtcgggc cgagaaatgg ccttcctcgg agtcatgacc tccaccagag gtggaggcat
     4861 gaaatacccc tgaaccgctc aaagcggatt gaaatgagcg ccacctaaag gtggccatcg
     4921 cctggacgcc aataccaggc ccgggggcaa gggtctgggg ttcaaaaatc atgaggcgtc
     4981 cttcatttca tgggccgggt cggccagccg atcccgcaac ccctggaagg tcttgtcctt
     5041 gtaggggcga ccggccggtc gcccgaccgg gccgccggct caagcggaag ccagtcccag
     5101 cctccaggaa tagggatcgc ccggccgatg ctttttgaac cccagactct tgcccccggg
     5161 ccacaactca ggcatcccgg aaatgtcaaa ctctttgagt cccccggcag agccgggggt
     5221 ttaccagaat aaaattatcc ccccccgacc tttcatcctc ctttctgcaa gggcagcacg
     5281 atgtgagagg gataggcggc gtcgtggtag acggcctggt gggcgaggtt gtagcgctgc
     5341 tccaggccca gcgggccgcc ggtgttgggg ttgacgtcga accgcggcca gttgctgcta
     5401 ctgatgtcca gtcggatgcg gtgccccttt ttaaaaatgt tggatgtggg gtatagctgg
     5461 aaaacgaact cataggcctt tccgggttcc agaaactccg gtttctcaaa tccgtttcga
     5521 tatcgggctc ggaggatgga atcggtcagg ttgatggcca agccgtcggg ctcatcctcg
     5581 tcgggcggat agacgtcgat gagcttcgcg gtaaagtccg tatccgcggc cgacgaggag
     5641 gcccacaggt gcatttcgat aggtcccgta atttccacat cagactccag cgggggagtc
     5701 tggaaggtca gtatatcgga ccgcaaactc agcggctgtt tgtcggtgca acctatgctg
     5761 ccgcttcggc cgcgctgatc gaaggcgccc ggcctcatca cgatatcgac cgccgaaatc
     5821 ccgccgccga gagtaggcac cgggtcgcgg gggttgaagg tgtatcgact ggggggaatg
     5881 tccggtttcg cgggcttttc cgcagacagg tttccatcgg cttggaggta gtagggggtg
     5941 aaccgtgtgc cggggatggg ccagtcatcc ataccccgcc agaagccgcc gtggtggatt
     6001 ctgcctggat agtccgacgc ctcccccggc gcaccgtcga tgacccgcct cccgccgcca
     6061 gttcccatgg taaagatttg aaccggtcgc cagtcggcca cctcggtgtt catgcctttc
     6121 aaaaagcgat cgaaccaggc cagctttaaa tcaaggtagt taatctccgc ttccgtgcca
     6181 aagtcgaggt caccagagta ggtaatctca tacttcccgt gggtccatgg acccatgaga
     6241 aggtactggg gggatttttt gatctttcta agtcgcacaa agctttcaca ggtgttgcgg
     6301 gcataggaat cataccatcc tcctaaataa agagtgggaa catcggcatg ctcccggtag
     6361 tactccgaga tcgcgtaccc gcggtgttcc ttccagtagg agtcataatc cccgtgggtg
     6421 agaatgtcca gggcccactg ctcgtaagac ggtaacctac gcaagagggt tgcccctttc
     6481 ttgagcggag accttcgcag gatctcgggc atctcttccg tataggcttt gatgagggcc
     6541 tttttaagat tcgggtcagc ggccgcttcc ttgctggtga tcgccatgcg gaagacataa
     6601 acatgaaacc gcagctccag agcgccgtta tgccgcatgg aactgtggta gtagttggaa
     6661 gcccccacgg ccacgatcat ggtggagagg tggggcgggg aaagggtggc cagggcgctc
     6721 tggtcgcttc cggcgtagga gtctcccatg gtacctacct tgcccgcgct ccatttctga
     6781 gctccgagcc actccacagt gtcatagccg tcgtgggctt ctttggcgaa agcataccaa
     6841 tccccttcag aatcaaagcg tccccgcacg tcctggatgg cgcacacgta tccccggcgg
     6901 gcaaagtatt tccctttcaa gacgctcccg gatgagcctt tgccgtaagg ggtgcgctcc
     6961 agaatcacgg ggaatttccc cgccgcctgt cgatcgcctg atgctgggaa gtagaggtcc
     7021 gtggccagct tgacgccgtc ccgcatgggg atcatggcac tggagtcgat gagcgcgtcg
     7081 tactgttgat gagaaccttc gtaggattgg ggcatggggg gctcctttcg atcgggtttc
     7141 ttaagtaaaa aaatcccctc tccccttgcg ggagagggtt gggtgagggg tataaaaatg
     7201 aggatttttg aggttctccc aatccttccc acccccaccc atccctcccc cgtcgagggg
     7261 gaggggaatg aagatgaagg aacttgtggg tataagccaa aggttggggc aattcatgaa
     7321 atgccccgat gaagcaaatg cgctccttcg cgccgcaggg tggattgaaa ctgaaacccg
     7381 cccctacctg ggctcgagaa tttgggggac tgctgcctcg gctttcaccc tatcccacgt
     7441 gtagcgcata gggatcagtt ccagattgcc ccagatggaa gcctgatcgg catagtgccg
     7501 gcttccagga tggccagaga cgcccaaggg cacggcccag ccggaattgt cccagtcgga
     7561 gatgtcaaat atatagcgga ccacggagag aagcgttata tcaaaaggac ggccgggcga
     7621 atagccggcg gcatagggcg tatctccatc ccctcccatg gatacggggg gcgggttcag
     7681 gaagttctcc agcccgggga aaagatggga gatcggatgt ttgggggacg ttcgatggat
     7741 cgtattccat ttccaagtct ccatgtcgcc tccaaggcgc tggcggagat aagcaaccgc
     7801 ttcattcagg gcctcgccag ctatcgatcc ccatgtgacc ccctggggaa gaaaagcggc
     7861 atcgcccgtc tttgcctggt gaacgatttg ggacgccagt tcgcccaggt gccttggggc
     7921 gccgcgtccg gtggagttaa acatcacgtc caccagcggc cccatccggc tgccgatgat
     7981 tttatggaga agtttgactc gaaaggcgct gtagatcgtc ggggcgacgg cgtcccgctc
     8041 catggcgcca ttccagcgga caagcatgga ttgcgctttg atcgagaatt cgttggttag
     8101 cttcgcttcg gcgataagct taatgaatac ctgcgcgggt atggatatgg aatccccatg
     8161 gatggagcgc atatcctcca tggtggcccc ggtcaggttc tccagccgct cgtaaatccg
     8221 ccgggcccgg tattcggggg catagcttag ggcgatatag tatggatatt tctggtccgg
     8281 gatccgattg ttggcggaga cgatgaatcc cgtggaaggg ttacgcagcc tggatagttt
     8341 ttcgaaaggg atacttccct gccattcgtg gtctccgctc cagcccggga ccggcatcca
     8401 ggcattggac atggaccgga tgggcacctg cccccggtgc aggtaagcga tgttcccgtg
     8461 gacgtcggcg gaaacgagat tgttgcaggg gtccacccat ttccgcatgg attcgttcaa
     8521 ctcgtccacg gaggccgctt tcatcatggg gagaaggcat tcaaaccccc tgtaggggaa
     8581 agccgtggcc gtgtactgaa acgccaggcc gtaacccttt cccggacccc ctgcgatgat
     8641 cgggccgtgg cgcgtaaggg tcacttccag ggcctggtcc gtttcgtctt tcaccttgat
     8701 tcgctcccgc cggacttcgg cctctttcca ctgaccccga aattcatagc gagaagctcc
     8761 ttccggagag aaccgttcga tgtaaaggtc ctggtaatcg gcctgggcgt gggtgacgca
     8821 ccaggccaca tggggattat ggccgaagtg ggcaaagcct gggactccgg gaaaggagat
     8881 ccccatgacg tcgaattcct tacaggcgat gtggttctgg tagtaacaat tcggggtatc
     8941 caggaggcga tgggcgtcgc ccgccaggaa aggctttccc gatgaggtcc ggctgccatg
     9001 gacggcccag ttgttgctgc ccgaatccgg ctcatcctga agccagcgaa tgccctccaa
     9061 atttcggctc agttcttcga gcccgtttag aactggacct ttatatgttc cgccgggggg
     9121 cgcgatcact agatcgccgg gcgggtggcc ccccaacaga gccgccgcct tttccgtgcc
     9181 cagggtgttc accagcttgg cgcgccagag ctttccctcg aacacgccca tcataatgtg
     9241 gcgcactttg aacacggcaa aagagtcccg gggatgccag ggctccggcc ttgcatcgag
     9301 cagcttatat tcgatgggca gcgcgtccgt gctttcgatg aacgcgttga ccccggccgc
     9361 gtaagcatcc agcatggccc gggtgggggc gtcgacggac tggtaatcgt tttcaaccgt
     9421 tgctccgatc tgaaagcggc gcatcacttt gtccccttca ac
//
LOCUS       DASPRR010000185         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2301685, whole genome shotgun sequence.
ACCESSION   DASPRR010000185 DASPRR010000000
VERSION     DASPRR010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301685"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VEL68_07165"
     CDS             complement(<1..536)
                     /locus_tag="VEL68_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXZ35779.1"
                     /translation="MKIHVGTSGYAYKEWKGKFYPEKVSPKEMLRYYSGRLDTVEINN
                     TFYHMPRESVLISWAGQVPDDFVFALKAPQVITHLKRLRNVVQETEYLFTTLSVLEGK
                     LGPVLFQFPKSFRADRTALQDFLGLIPGGMPCAFEFRSPSWLDDEVLDLLRKRKSSLC
                     IADADENPANEIISTAPWG"
     gene            complement(541..2151)
                     /gene="ligD"
                     /locus_tag="VEL68_07170"
     CDS             complement(541..2151)
                     /gene="ligD"
                     /locus_tag="VEL68_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXZ35780.1"
                     /translation="MGLQEYASKREFEKTREPKPGLGHDGGRLIFVVHKHAARALHYD
                     LRLELEGVLKSWAVPKGPSLDPSLKRLAVMVEDHPFDYKDFEGVIPEGNYGAGSVIIW
                     DRGFYHHPSAQDEKESQKFLLDGFRKGNLKFVLEGEKLRGEFALVKTGKDGKSWLLLK
                     KKDRNATQGDILKENRSVVSQKTLEEMLETGGSKSFRQKKLNQVRLHEAKESEDLKDA
                     PLKPMPQGVQPMLATLVKEPFDHPDWVFEVKWDGYRAVAEIREDGVSLYSRNLISLGK
                     KFFPIVDSLRKLRLEAVLDGEIVVVDDQGRPDFQRLQHYQDLGNGHLLYYVFDLLYFR
                     GHDLTGLPLLRRKELLKKILPPTPRIRFSDHVRAEGVLFYKAAKEKGLEGIVAKHSQS
                     VYEAGRRSHQWLKVKTQLTQEAVITGFTEPGGGRKYFGALVLGVYQGNELMYIGHVGG
                     GFTANHLRDIRERLEPLIQKECPLAVEPETNAPVTWVKPELVCEVALSGWTEDAVMRH
                     PVFLRLREDKAAREVVREKPLGSGDGGL"
     gene            complement(2532..>3227)
                     /locus_tag="VEL68_07175"
     CDS             complement(2532..>3227)
                     /locus_tag="VEL68_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXZ35781.1"
                     /translation="RCPSHHPAWVKTAEVPQEGGEPMTVCLVNDLETLIWAENLASVE
                     LHVPLARANSPERPDFMVFDLDPGDPAGLRECARVALILRDLLSRMGLASYVKTSGKK
                     GLHVFVPLNRKEMTFEDTKTFSKAVAGIMQKHYPDLVTARMAKKERTAKVFINWSQND
                     ASKTMICVYSLRAREKPTVSFPLEWGELENPAGSGDPERLQVIHGEALSRVEKKGDLF
                     REVLVKEQKLPHL"
BASE COUNT          694 a          952 c          803 g          778 t
ORIGIN      
        1 ccccatggcg ccgtgctgat gatttcattc gccggattct catcggcgtc ggcgatgcac
       61 aggctggatt tccttttgcg aaggaggtcc agaacttcat catcgagcca ggatgggcta
      121 cggaactcga aagcgcacgg catacccccg ggaatcagac cgagaaaatc ctgcagcgcc
      181 gtccggtccg cacgaaaact cttgggaaac tgaaagagaa ccggccccag tttcccctcc
      241 aggaccgaga gcgtcgtgaa gaggtactcg gtctcctgga ccacattccg cagccgtttc
      301 aggtgcgtta tgacctgggg cgctttgagc gcaaaaacaa aatcatcggg gacctgcccg
      361 gcccaagaga tgagcacgct ctctctgggc atgtgataaa aggtattatt gatctccact
      421 gtgtcgaggc gccccgaata atagcgaagc atttccttgg gcgagacctt ttcaggatag
      481 aatttgcctt tccattcctt gtacgcatac ccgcttgtac cgacgtggat tttcatggtt
      541 ttacaatcct ccatcaccgc tcccgagtgg cttttcgcgc accacttcgc gggcggcttt
      601 atcctcgcgc agccgcagaa agacggggtg cctcataacc gcatcttcgg tccagccgga
      661 taaggcaacc tcgcagacca gctccggttt aacccaggtg acaggggcat tggtctccgg
      721 ctccacggcc aaggggcatt ctttctggat caggggctcc agtctctcgc gaatatccct
      781 taaatggttc gccgtgaatc cgcctcccac atgaccgatg tacatcaatt cgttcccttg
      841 gtatacgccc aggaccaggg cgccgaaata ttttcggccc cctcccggtt ctgtgaatcc
      901 ggtgatcacc gcctcctggg tgagctgcgt cttcaccttc agccattggt ggcttcttcg
      961 gcctgcttcg tacacgctct gagaatgttt ggcgacgatc ccttccagcc ccttttcttt
     1021 cgcggccttg taaaataata cgccttccgc ccgaacgtga tcgctgaatc gtattcttgg
     1081 agtgggcgga agaattttct tcaagagctc tttcctccga agcaggggca agcccgtcag
     1141 atcatgcccc cggaaataga ggagatcaaa aacgtagtag agcaaatgac catttcccaa
     1201 gtcctggtaa tgctgcagtc tctgaaaatc gggacgcccc tggtcatcca cgacaacaat
     1261 ttcgccgtcc agaacagctt ccagtctcaa tttccgcagc gaatccacaa tcgggaaaaa
     1321 ctttttgccc aacgagatca ggttccggga atagagcgaa acgccgtcct cccggatttc
     1381 ggcaactgcc cggtacccgt cccattttac ctcgaagacc caatccggat gatcaaacgg
     1441 ttctttgacc aaggtcgcga gcatcggttg tacgccctga ggcatgggtt tgagcggcgc
     1501 atcctttagg tcttcgcttt ccttcgcttc gtgaagacgg acctggttca atttcttttg
     1561 tcggaacgat ttcgaccctc cggtttcgag catctcctcc agggttttct gggacacaac
     1621 ggaacggttc tccttgagga tgtccccttg ggtcgcattt cgatcctttt tcttcaggag
     1681 aagccacgac ttcccgtcct tccccgtttt taccagcgca aattcacccc gaagcttttc
     1741 cccttcgagg acaaatttca ggttcccttt tctgaatccg tccagaagaa atttctggct
     1801 ttccttttca tcttgggcag aagggtgatg atagaagccc ctgtcccaga tgatcacgct
     1861 tcctgcacca taattgccct ccgggataac gccttcgaaa tccttgtaat caaacgggtg
     1921 gtcttcaacc ataaccgcca gcctttttaa ggagggatcc agcgacgggc ctttcggcac
     1981 ggcccagctc ttcagaaccc cttccagctc cagcctgaga tcataatgaa gggcacgggc
     2041 cgcatgctta tgaacgacaa aaatgaggcg gcccccgtca tgtccaaggc ccggtttcgg
     2101 ttcacgcgtt ttctcgaatt cccttttgga tgcgtattcc tgtaatccca tattgaaaac
     2161 ccattcggtg caaatctaaa aaaacatttc agccaccgaa gccagggaga attgaatttc
     2221 taaaattata acgactcaaa cagcagtcat ttcgagcatc gagccgcaaa gcaatccccc
     2281 caccctctct ctccccctcg agagactgtg tcataatcgc ccggtcgccc ttcgacgggc
     2341 tcagggggaa cggctaagtg gctgaattgt aatgagccca taatccgttc gtggtgagct
     2401 tgtcgaacca tgaacggatt acgacacagt ctccgagggg ggagggttgg ggggggtgag
     2461 attcccgcgc gcctcgggct ggcaacgact gtaacaggat tatttcattt gtattagttt
     2521 tcacgatctt ctcataagtg agggagcttc tgttctttca cgagcacctc tcgaaaaagg
     2581 tccccctttt tttcgaccct gctcagagcc tccccatgta tgacctgcaa cctttccgga
     2641 tcgccggacc cggctggatt ctccagttcc ccccattcaa gcggaaaaga aacggttggc
     2701 ttttcccggg cgcgcaggga atagacgcag atcatcgttt tcgaagcgtc gttttgagac
     2761 cagttgataa aaaccttcgc ggtgcgttct ttcttggcca ttcttgcggt caccaggtcc
     2821 ggataatgtt tctgcatgat tcctgccacg gctttggaaa aggtcttggt atcctcaaag
     2881 gtcatttcct tgcggttcaa agggacaaac acatgcagtc ctttcttgcc tgaggtcttg
     2941 acatagcttg caagccccat tcgggagagc aggtcccgaa ggatcaaggc cacccgggca
     3001 cattcccgga gccctgccgg atcgccgggg tccaggtcga agaccatgaa atccggcctc
     3061 tccggggaat tggccctggc cagcggcaca tgcagttcaa cagacgcaag gttctccgcc
     3121 catatcagcg tttccagatc gttgaccagg cagaccgtca tcggctctcc gccctcctgg
     3181 ggaacctccg ccgttttcac ccaggccgga tggtgagagg gacagcg
//
LOCUS       DASPRR010000186         6691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1821345, whole genome shotgun sequence.
ACCESSION   DASPRR010000186 DASPRR010000000
VERSION     DASPRR010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821345"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VEL68_07180"
     CDS             <1..567
                     /locus_tag="VEL68_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXZ35782.1"
                     /translation="IPTREEALKLLHQYFETDSLRKHAYAVEGVMRYIARKRGEDVEK
                     WGIIGLIHDLDYEKFPDQHCKKTREILEGEGWAEEYIRAAVSHGWGICSDEEPQTILE
                     KTLYAIDELTGLVVTTALVRPSKSVMDVAVKSVKGKWKDKRFAAGVDRSVIEKGAAML
                     GVPLEELIDDTIKGMQEVAEAIGLKGAS"
     gene            735..1430
                     /locus_tag="VEL68_07185"
     CDS             735..1430
                     /locus_tag="VEL68_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXZ35783.1"
                     /translation="MKSPKSTLCLKSFEEMKGLLESRSFSLPEPPLLPPPMMEAESEP
                     ENEENLFRQAMEGVTRIPRPNFVERGLPPPAPEKPQTVTETEPLLKLKDLVQHGMGFE
                     VADTPEYIEGTMYHVHPEVARRLHQGDFSIQAYIDLHRFKVADAREAFERFIKWTVTT
                     GKTGILVIHGRGLSSSSEPVLKHKVVEWLTRGPWRKWVVAYATARSCDGGAGATYVLL
                     RRRPVSKRIKKGR"
     gene            complement(1499..2560)
                     /locus_tag="VEL68_07190"
     CDS             complement(1499..2560)
                     /locus_tag="VEL68_07190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ35784.1"
                     /translation="MRVLILSPTTLPSITGNAVTAERWRRALKGEGVIVQTLATRYVA
                     ASILKEGIDYFRPDLIHAHHAFQSGCRLLDLSFPGAGKKIPFVVSPAGTDINKDWNAA
                     ARREAISRVFESARAIITQGPGTLERVKEVLPSPDGRVFPVPKSILWFGDDDYRLRDH
                     AKCLPGDVLFLLPAGVRPVKGNLECLMAMEKVHEFRPQVRVVFAGPELDPVYTARFAE
                     EVRRCAAFACWIPLIPPAAMRSAYEAADVVLNASFSEGLSNSLLESMASGRPLLASDI
                     PGNRGPIWGETGEEPAGLFFDPLDPEDFIKQALRLIDDQTLRNSLGRAGKRRAATWPG
                     LKEEADGLIRAYEFARQTS"
     gene            complement(2569..3432)
                     /locus_tag="VEL68_07195"
     CDS             complement(2569..3432)
                     /locus_tag="VEL68_07195"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HXZ35785.1"
                     /translation="MKRLVVNADDLGVDEARNAGIFEAVRAGSVTSVSILANGPALEG
                     ALQVVRSLPQKKVSWGIHLNLSEGTPLSAGLSLLTGKEGSLLGKARTHKLLSNPGNPE
                     LEKQVAQEIETQIQILLKTGVRISHLDGHQHVHVFPAAFRAAVRAARIHRIPWVRIPE
                     DPGPNLDAPGIQDSLIAEARNFSRLGAEARLHLKGSGVSTTDYFRGLYLKGKFTLPIM
                     EENLRQLPPGLTELMVHPGRVPMAPIPSPFSAFSTLDREKELETLLDNRFRLALDQNR
                     IELIPFPGTED"
     gene            complement(3429..5015)
                     /locus_tag="VEL68_07200"
     CDS             complement(3429..5015)
                     /locus_tag="VEL68_07200"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXZ35786.1"
                     /translation="MADSEEIELRAAELARECDLVIDHTDTFWGRGLFRPLVRLLLEM
                     EGARVVGSDSRACFLADDKIAAKTRLGQSGISVPPGIVVRAAEEKIPDWLKPPLVLKP
                     AFEHMSRGLEMARSEEEARAMASELLSRLKQPILMETFVPGRELAVSLLEGPGGIQIL
                     PPLEWRFEKMGSVVLTEAFKLKEVVGERRDARQADLAPPLGDELESLARRAFLALGLR
                     DYARFDLRLSPGGTFFFLEANTTPSLEPMEALSLSAKWAGLDYPSLVERMLSAALRRY
                     GNPPGGGEQRIRIDLPTGTVELKVPRGVHFPPPSSIDLARILDIRAGERVLDLGCGTG
                     LLSIAAAKGGARRVVATDLDPQALDATMINARANRVERQIEVRAGSWYDTLGWGAAER
                     KAERFEAIIATPPQTPGPYPFGPRYGGRDGTRHLFEVIEGAPRWLDPSQGRLWLLAIS
                     LANPPALMKKLREFFFEVSVVKETDRWFTPEEYESLEKGLFEHFLALRSSGQSEFQEE
                     GRGRYVFRNLFIRAAGVKAR"
     gene            complement(6069..6539)
                     /locus_tag="VEL68_07205"
     CDS             complement(6069..6539)
                     /locus_tag="VEL68_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HXZ35787.1"
                     /translation="MARKSDFPPFKVLRNEQLLELAIQKPLHLEELEAVKVLSRKQIE
                     RHGTHLLQGIHRAMAIPPADLPVYPRVGRTDLPSAVRKRAKALKTWRELRAKSLGMEP
                     GILLNNALIHDLALKNPHSIEELAEISGLKNWRQRHFGREILNAQTGGQKGDGT"
BASE COUNT         1531 a         1922 c         1846 g         1392 t
ORIGIN      
        1 attcccaccc gcgaagaggc cttaaagctt cttcaccagt attttgagac cgacagtctg
       61 cgcaagcatg cctatgcggt cgaaggggtc atgcgctata tcgcccgcaa gcgcggcgag
      121 gatgtagaga aatggggaat catcggcctg atccacgacc tcgattacga aaagtttccg
      181 gaccaacact gcaaaaagac ccgggagatc ctggaaggag aaggctgggc ggaagaatac
      241 atccgcgccg ctgtctccca cggctggggg atctgcagcg acgaggagcc ccagaccatc
      301 ctggaaaaaa ccttgtacgc catcgacgag ctgaccggcc tggtcgtcac cacggcgttg
      361 gtgcgccctt ctaagagcgt aatggatgta gcggtgaaat ccgtaaaggg caaatggaaa
      421 gacaaaaggt ttgccgcagg cgtcgaccgg tcggtcatag agaagggagc agccatgctg
      481 ggagtccccc tcgaggaact gatcgatgac accatcaaag gaatgcagga agtagccgaa
      541 gccatagggt tgaaaggcgc ctcctgaacc attcaggacc cccctgcccc cccattccct
      601 gggcaggggg atcgccaacg gccccgtcga ttccccctcc cctcagccgc ccggatgaat
      661 tttttgataa ttcgccctct ttgagataga ataaatccag atggtggaga atgcttcctg
      721 gaaagggacc ggcaatgaaa tctcccaagt ccaccctctg cctaaaatcc ttcgaagaaa
      781 tgaagggcct cctcgaatcc aggtcctttt ccttaccgga gcctcccctt cttcctcccc
      841 ctatgatgga agcagaatcg gagcccgaaa atgaagaaaa cctcttccgg caggctatgg
      901 aaggggtaac ccgcattccc cgccccaact tcgtcgaaag ggggcttccc ccccctgccc
      961 cggaaaagcc ccaaaccgtg actgagactg aacctctcct gaagctgaaa gacctggtgc
     1021 agcatgggat gggattcgag gtcgccgaca cgcccgaata catcgaaggc acaatgtacc
     1081 atgttcaccc ggaagtcgcc cgccgccttc atcaggggga tttttccatc caggcctata
     1141 tcgatcttca ccgtttcaag gtggccgatg ccagggaagc ttttgagcgc ttcatcaaat
     1201 ggacggtgac cacggggaaa accgggattc tcgtcatcca cggccggggg ctttcttcct
     1261 cctcggaacc ggtcttgaag cacaaggtgg tagaatggct cacccgcggc ccctggagaa
     1321 aatgggtggt cgcctacgcc accgcccgat cctgcgacgg gggcgcggga gcgacctatg
     1381 tgctcctgcg cagacgcccg gtctccaaaa gaattaagaa aggccgatag aaaatcggcc
     1441 tccctcacgg cgggaaaaga ggatcggcac cctctttgtc catggcggat ttacaaggtc
     1501 aggaggtttg ccgggcaaat tcataagccc tgatcagccc atcggcttct tccttcaggc
     1561 caggccatgt cgccgcccgt cgcttccccg cccgccccag agaattcctg agtgtctgat
     1621 cgtcgatcaa gcgcaaagcc tgcttgatga aatcctccgg gtctaggggg tcgaagaaga
     1681 ggccggctgg ttcttccccc gtttcccccc agattggacc gcggttcccg ggtatgtccg
     1741 aggccagcaa gggtcttcct gatgccatgc tctccaggag ggagttggaa agtccttcgg
     1801 agaaagaggc gtttaaaacc acatcggccg cttcataggc ggaacgcatg gccgcggggg
     1861 gaatgagggg aatccagcac gcaaaggccg cgcatcgccg gacctcctcg gcaaagcgtg
     1921 cggtatagac cgggtcgagc tcagggccgg caaaaactac tcgcacctga ggcctgaatt
     1981 cgtgcacctt ttccatggcc atgaggcatt ccaggtttcc tttcaccgga cggacccccg
     2041 cggggagaag aaagaggaca tccccgggaa gacattttgc atggtccctc agccggtaat
     2101 catcatcgcc gaaccacagg atcgacttgg gaaccgggaa aacccgtcca tcggggctgg
     2161 gcaggacctc tttcacgcgc tccagggtcc cgggcccctg ggtgatgatg gcccgggccg
     2221 actcgaaaac ccgggaaatg gcttctctcc tggcggccgc gttccagtct ttgtttatat
     2281 ccgttcccgc aggagaaacc acaaagggga ttttcttccc cgcgcccggg aaggaaaggt
     2341 ccaggagccg gcacccggac tggaaagcgt gatgggcatg gatgaggtcc gggcgaaaat
     2401 aatcgatccc ctccttcaga atggaggccg ccacataccg ggtagccagg gtctgaacga
     2461 tcactccctc ccctttcagt gcgcgtcgcc atcgctcggc agttacggcg tttccggtta
     2521 tggaagggag ggtcgtggga ctaaggatca ggacgcgcat agaagaaatc aatcttcggt
     2581 ccccggaaaa ggaatcagct cgattcggtt ctggtccaag gccagacgga aacgattgtc
     2641 taacagggtt tcaagctcct tttcccgatc caaggtcgag aaggcggaaa aagggctggg
     2701 aatcggggcc atcggaaccc ggcccgggtg caccatgagt tcggtgagcc ccggagggag
     2761 ctggcgcaaa ttctcttcca tgatcgggag agtaaatttt ccttttagat agaggccccg
     2821 gaaataatcg gtcgtgctta ctccactccc cttcaaatgg aggcgagcct ctgcgcccag
     2881 tcggctgaaa tttcgagctt cggcgatcaa ggaatcctga atccccggag cgtcaaggtt
     2941 cggacccggg tcttccggaa tccggaccca cgggattcgg tgaatccgcg ccgctctcac
     3001 ggcggcgcgg aacgctgccg gaaaaacatg gacatgttga tggccatcca ggtgagagat
     3061 tcggaccccg gttttcagca gaatctgaat ctgggtttcg atctcctgcg ccacctgttt
     3121 ttccagctcg ggattccccg ggttcgaaag aagcttatgg gtccgggcct tgcccagcaa
     3181 agacccttcc ttcccggtaa ggaggctgag ccccgcagaa agaggagttc cctcggaaag
     3241 gttgagatga attccccagg acaccttttt ctgggggagg gaccggacca cctggagggc
     3301 gccctccagg gcaggtccat ttgccaggat gctgacgctg gtcaccgacc ctgcccggac
     3361 cgcctcgaag attcccgcgt tgcgggcttc atctacccct aggtcatcgg cattgacgac
     3421 gagtcgcttc accgcgcttt cactcccgcg gcgcggataa aaagattgcg gaaaacatat
     3481 ctccctctcc cttcctcttg gaattcggac tgcccagagg agcgtagggc cagaaagtgt
     3541 tcgaacagtc ccttctctag ggattcatac tcttcaggtg taaaccatcg atccgtttct
     3601 ttgaccaccg aaacctcgaa aaaaaactcc ctgagcttct tcatgagggc gggcggattg
     3661 gccagggaga tggccaggag ccagaggcgg ccctgggaag ggtccaacca gcggggggcc
     3721 ccctcgatca cctcaaagag gtgccgagtc ccatcccgtc ctccgtagcg cgggccgaac
     3781 ggataaggac cgggagtctg gggaggggtg gcgatgatcg cctcgaaacg ctcagctttt
     3841 ctttccgcag ccccccagcc caaggtgtcg taccaggagc ccgcccggac ctcgatttgc
     3901 ctttccaccc ggttggccct agcgttgatc attgttgcat ccagagcttg agggtccagg
     3961 tccgtcgcca cgacccgccg cgcaccccct ttggccgcag cgatggataa aagccctgtt
     4021 ccgcacccaa ggtccagcac cctctcccct gctcttatgt ccagaatcct cgccaagtca
     4081 atgctcgagg gcggggggaa atggaccccc cgcggcacct tcagttcgac ggttcccgtg
     4141 gggagatcga tccggattct ttgctcccct cctccggggg ggtttccgta acggcggagg
     4201 gccgcggaaa gcattctctc taccaaagaa gggtaatcga gtcccgccca tttggcggaa
     4261 agggacaggg cctccatggg ctccaggctg ggagtggtat tggcttccag aaagaaaaag
     4321 gtgcctcccg gagataacct cagatcgaag cgggcataat ccctcaagcc gagagccaga
     4381 aaagcccgcc gggcaaggct ttccaattca tcgcccaagg gcggggcaag atcggcctgc
     4441 cgggcatcgc gcctttctcc cacaacctct ttcagcttga aggcctcggt cagcaccaca
     4501 ctacccatct tctcgaatcg ccattccaag gggggtaaga tttgtattcc tcccggccct
     4561 tcaagcaacg aaacggccag ttccctacca gggacaaagg tctccatgag gatgggctgc
     4621 tttaaccgag acagaagctc ggaggccatt gcccgggcct cttcttcgga cctggccatc
     4681 tcgagccccc ggctcatgtg ctcaaaagct ggctttaaaa ccagcggggg tttcagccag
     4741 tcggggattt tttcttccgc ggctcgaacc acgattccag gcgggacgga aatcccactt
     4801 tgtcccagac gggttttggc cgcgatcttg tcatcggcca aaaaacaggc ccgagaatcc
     4861 gaaccgacta cccgcgctcc ctccatttcg agcagcagcc ggaccagcgg ccgaaaaagg
     4921 ccccgccccc agaaggtatc ggtgtggtcg atgaccaggt cacactctcg agcgagctca
     4981 gcggctcgaa gctcgatctc ctccgagtcc gccaaaaaga attcatggcc gagggcctta
     5041 agcccctctg cgaccgactg ggcgcactga cagggagatt ggacagtcct gacaatcccg
     5101 actcgcatag gaaaggaata tgattttggg gaatgaataa atctttatgg aaatagccta
     5161 tcggagggta tggggatcgt caaggttaaa gatcagttcc tgttgcagtc atggaataga
     5221 aagggctgag ggaatttttt gaaaagcatc ctcggcattg agcctgggaa gaccctcctt
     5281 cgggataatg gcttaaccag gatggcggga tcaggattgg ggtcgaagga aagtgaagtc
     5341 cctcccattt tcaagcataa tctaagattc gcaggccgga aggtttaccg ctttagggga
     5401 acgccctggt cgatttaatt ggccaaatac tttcccgatt ccgaatgcat aggataccga
     5461 taaaagcgct cagggaagga aacgttcctc tgcgtcgtcc tgctgcacac cagacaggcc
     5521 aggataaaac ccaagaaacc cccggaaaaa aaggctataa cgatagccca taacatattg
     5581 aagggctcct ttctccatta ttttttcccg gcttcaaatg aaagacagag ccaattatta
     5641 aaacccgttg gatgactata acccttttat gtgtaaagat ctagatcttt ttttacaatt
     5701 gtatcggatt ttgtgccaaa gtattgacat actaaccatg aaggatttcc atacatctta
     5761 tttattaaat aaaataaaat aattatacta accatttgaa agggcaaaaa acgattttta
     5821 ttcttacaat attgttcttt tccttatgca aatctggggc cgctggaaag aattcaaatg
     5881 gcggggtaat gaacctggcc ttgagtccca ggaagtttgg ggggtaaggg aggtattggc
     5941 tgcgaattat tgaagattat tacggggttc acgatgccat gttcggccgg cctcgaagcg
     6001 gagtgggaga atccatcaaa gaaaggggcc tacttcatcg ccacaccttg ccccaccacc
     6061 ctgagccttt atgtcccatc ccctttttgt ccccctgttt gagcattcag aatctcccgg
     6121 ccgaaatgac gttgccgcca gtttttcaag cccgaaattt ctgccagctc ttctatcgag
     6181 tggggatttt ttaaggccag atcatggatc agggcgttat tcaaaaggat tccaggctcc
     6241 atccctaaac ttttggcccg cagctctcgc caggttttca gcgctttggc cctttttcgt
     6301 acggcggagg gcaaatccgt ccttcctacc cgggggtaca ccggaaggtc tgcgggggga
     6361 atggccatgg ctcgatgaat tccctgcagc aagtgcgtac catgtctttc gatttgtttc
     6421 cggctcagaa cttttaccgc ttccaattct tccaggtgca aaggtttttg gatggccaat
     6481 tccaggagct gttcattgcg cagaactttg aaggggggga aatccgactt ccgggccaga
     6541 ccctagcgga attccagcaa aacctccagg atcgcaagac tcatggggtc caggctaaaa
     6601 gcccctttca ctcttaggta gagcgggtca tggctggggg gtgtaaatcg gaccttactc
     6661 agaattaggc attcttcctc cacccaggac a
//
LOCUS       DASPRR010000187         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2141619, whole genome shotgun sequence.
ACCESSION   DASPRR010000187 DASPRR010000000
VERSION     DASPRR010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141619"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..1584)
                     /locus_tag="VEL68_07210"
     CDS             complement(385..1584)
                     /locus_tag="VEL68_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35788.1"
                     /translation="MDTESKNRSLGVLLAVMTLSHAVSAFCNLSIPPLTPFLRDELNL
                     THAEVGMLMSFIYIGVVSSSLFFGWITDLLGERRVLTLGLGIQGLFMIGFAWVHAFLI
                     GGVLLLLSGIGYSGVNPATTKGVMRWFPPQGRATAMGIKQTGIPLGGILASSTLPALA
                     LAFGWRLSVILVSAATLVSILAVRIGIPLAPPLEDRSSGMRWGLLREVLFNRSILALS
                     IMGIFLSGAQLAIITHLVLFLKKEFLFSTVLAGLLLALVQAGGIAGRIGWGLVSDFLA
                     GGRRKSILMIIGIIAVLQLFLLGRIGPDIPSILLVVFIALLGSTTIGYHGVLFGLMGE
                     IVRKEVVGLATGFSLTITFLGVVLYPPLFGHVVDRLGSYSLAWDMLALSWIAALFILF
                     FFVQERK"
     gene            complement(1614..2900)
                     /locus_tag="VEL68_07215"
     CDS             complement(1614..2900)
                     /locus_tag="VEL68_07215"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ35789.1"
                     /translation="MIVLFLFCVFLGLLCLGLPIAFVLGVTAFIMIAFFSPAPLQMVP
                     EVMYNSLDTFPLMAIPFFVIAAQFMVQGGTSRYLINAANCWVRHWWGGLAMVTVISCM
                     IFGALCGSSVATALAIGVIVVPAMIEHGYPRPFALGVISAAGTIAIMIPPSIAMIIYG
                     IMADESIPRLFMAGFLPGIMQGIFYMIWIRFDARRKNIGGAPKASWEETFWTSYKAIP
                     AFCMPVIILGGIYSGFVTVTEAAALGAVAAIFISLFIYREVSFGKVIPLAGDAMKSAG
                     MIMFIISTAITIGNWLTEAGLPAKLVEIAKDMNLTAFWFLLSVNIALLILGCLLEVVS
                     IMLITLPIILPVLKALDINLIHFGIVMTLNMEIALITPPVGLNLYVISGVGKAPLAET
                     IRGVFPYIIITIIQLMLITYWPAYTLFLPNLIMGAR"
     gene            complement(2897..3451)
                     /locus_tag="VEL68_07220"
     CDS             complement(2897..3451)
                     /locus_tag="VEL68_07220"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ35790.1"
                     /translation="MSVWEKGWTRVEKFIVGLLALSATLIAFYGVITRYVFNRPPDWA
                     EEICIYMIIWGVFISASILAAEKGHVAATLLVERFPMKTRRYLAVFNGVVALAFCASV
                     AYLGLRIVAAAYINDERSVSALRFPVWIPYLSVAAGCILVGLRYAIRVYRLVFYFKPS
                     EIMETHELSRDGGYEDLSKDGGYE"
     gene            complement(3522..>3756)
                     /locus_tag="VEL68_07225"
     CDS             complement(3522..>3756)
                     /locus_tag="VEL68_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35791.1"
                     /translation="TFSDVWNEIVPKQREIARKEQAEARKLMEGKGVEFFDPSDEELA
                     KWRNYLMPIQGKIVKDLKHDTALVEMARKALGM"
BASE COUNT          920 a         1042 c         1104 g          690 t
ORIGIN      
        1 ccagcgccgg tttcagggtc ttctcctccc attgtttttt ctcctgtttg attttttcca
       61 ggtcttcctt catctgaaac ctccttctcc aatggagact ggaaaatgga cattgggtga
      121 aaggaaaaaa gaaatcctca atccgaacat gcaaagaata tcgaggttct tgcaaatcgc
      181 gaaaacagcg gaagttgtca ttccgggcaa gcgaagcgcg acccggaatc caggattatc
      241 aaagaaatct ggattccggt tcccggctta aaacctgccg ggacaagttt cgccggaatg
      301 acgcagtagg tgacttttgc aagagtctct ctcgaattta gaattttaat ctatatcaat
      361 gcaagaaaaa aaagtagttc catattattt cctctcctga acaaagaaga acaggatgaa
      421 taaagccgcg atccaggaaa gcgccagcat atcccaggcc agggagtagg agcccaaacg
      481 atccaccacg tggccgaaaa gggggggata caggacaacc cccaaaaagg tgatggttaa
      541 ggaaaatccg gtggccaacc ccaccacttc cttccggacg atctccccca tgaggccgaa
      601 aagaactccg tggtacccga tggtcgtgct ccccaggagg gcgatgaaaa caacgagaag
      661 aatggatggg atatccggcc cgatccggcc caggagaaaa agctggagga cggctatgat
      721 tccgatgatc atcaggatcg attttctcct cccgccggcg agaaaatcgc tgaccaggcc
      781 ccatccgatt cgtccggcga ttcctcccgc ttggaccaga gccaacaaaa gcccggccag
      841 gacagtggag aagaggaatt ccttcttgag gaatagtacc aggtgggtga tgatcgcaag
      901 ctgggccccc gacaggaata ttcccatgat ggaaagggcc aggatggacc ggttgaacag
      961 gacctctcgc aacagtcccc agcgcatccc cgatgaccga tcctccaggg gcggagccaa
     1021 gggaatcccg atccgaacgg ccaggatcga taccaaggtt gccgcgctca ccaggatgac
     1081 ggagaggcgc catccgaaag ccagggcgag ggcggggagg gtggaagagg ccaggattcc
     1141 ccccaaagga attccggtct gcttgattcc catggccgtg gcccggccct ggggaggaaa
     1201 ccacctcatc acccccttgg tggtggccgg attcacgccg ctgtaaccga tgccggaaag
     1261 aagaaggagg actcctccta taagaaaagc atggacccag gcaaaaccga tcatgaagag
     1321 cccctgaatc cccaggccca gggtcaaaac ccggcgctcc cccagcaggt cggtgatcca
     1381 accaaagaag agggaggacg agacgacccc gatgtagatg aaggacatca gcattcccac
     1441 ctccgcatga gtcaggttca attcatcgcg gaggaacggg gtaagtgggg gaatgctcag
     1501 gttgcagaaa gcggagaccg cgtgggaaag ggtcatgacc gcgaggagga cccccaggga
     1561 gcgattttta gattccgtat ccatgggggt ccgtattcga tctcaaccct ggctcatcgc
     1621 gcgcccatga tcagattggg caggaacagg gtgtaggccg gccaataggt aatcagcatc
     1681 aattggataa tggttataat gatataaggg aaaacccccc ggatggtttc ggccagaggg
     1741 gccttgccca cgccggagat cacgtaaagg ttcagcccga ccggcggggt gatcagggcg
     1801 atctccatgt tcagggtcat cacgatcccg aagtggatca ggttgatgtc cagggctttc
     1861 aaaaccggaa ggatgatggg cagagtgatc agcatgatgg agacgacttc caggagacaa
     1921 cccaggatca ggagggcgat gttgaccgaa agcaggaacc agaaggcggt caggttcatg
     1981 tctttggcga tctctacgag tttcgccgga agaccggctt cggtcagcca gttcccgatg
     2041 gtgatggccg tggagatgat gaacatgatc atccccgcgg atttcatcgc gtccccggcg
     2101 agggggatga cttttccgaa gctcacctcc cggtagatga acaggctgat gaagatggct
     2161 gccaccgcgc ccagggccgc ggcttcggtc acggtcacga acccggaata gatgccgccc
     2221 aggatgatga ccggcatgca aaaggcaggg atggccttat agctggtcca gaacgtttct
     2281 tcccagctgg ccttgggcgc gcccccgatg ttcttccgcc tggcgtcgaa gcggatccag
     2341 atcatgtaga agattccctg catgatccct ggaaggaatc cggccatgaa cagccggggg
     2401 atggattcgt cggccatgat cccgtagatg atcatggcga tgctgggcgg aatcatgatg
     2461 gctattgtcc cggccgcaga gatgaccccg agggcgaagg gccgcggata tccatgttcg
     2521 atcatggccg ggacgacgat gaccccgatg gccagggccg tggccacgct agacccgcac
     2581 agggcaccga agatcatgca ggagatgacc gtgaccatgg caagcccgcc ccaccagtgg
     2641 cggacccagc agttggcggc gtttatgagg taccgggaag tccccccctg gaccatgaat
     2701 tgggcggcga tgacgaagaa ggggatagcc atgaggggga aggtatccag ggagttgtac
     2761 atgacctcgg ggaccatctg gaggggtgcg ggtgaaaaaa aggcgatcat gatgaaagcc
     2821 gtcaccccca ggacgaaggc aatgggaagc cccaggcaga ggaggcccaa aaagacacag
     2881 aaaaggaaaa ggacgatcat tcgtaacctc cgtctttgct caggtcttcg taccctccat
     2941 cccggctcaa ttcatgggtc tccatgatct cggagggctt gaaataaaag accagccggt
     3001 aaacccggat cgcataccgc aggcccacca ggatacaacc agcggccacg gaaaggtagg
     3061 ggatccagac cggaaaacgg agcgcgctga ccgaccgttc gtcgttgatg taggcggccg
     3121 caacgatcct taagcccagg taggcgacgg acgcgcagaa ggccagggcc accaccccgt
     3181 tgaagaccgc caggtaacgg cgggtcttca tggggaaccg ctccacgagc agggtggccg
     3241 ccacgtggcc cttctccgcc gccaagatgc tggcggagat gaacacaccc cagatgatca
     3301 tgtagatgca gatctcctcg gcccagtcgg ggggccggtt gaagacatag cgcgtaatca
     3361 cgccgtagaa ggcaatcagg gtggcggaaa gagccagtag gcccacgatg aatttctcca
     3421 cccgggtcca tcctttctcc catacgctca tcaatgatcc tccgcggttt ttttgagagg
     3481 agaggggaat ccctctcccc ctccctatgc ttctcccatc cttacatccc cagagccttc
     3541 ctggccattt ccaccagggc cgtgtcgtgt tttagatctt tcacgatttt gccctggatg
     3601 ggcatgagat agttgcgcca tttggccagc tcttcgtcgc tcgggtcaaa gaattccacg
     3661 cctttgccct ccatgagctt tctggcctcg gcctgctcct tgcgggcgat ctcccgctgc
     3721 ttggggacaa tctcattcca tacgtccgag aaggtt
//
LOCUS       DASPRR010000188        15904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2302336, whole genome shotgun sequence.
ACCESSION   DASPRR010000188 DASPRR010000000
VERSION     DASPRR010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15904)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15904
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302336"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VEL68_07230"
     CDS             <1..631
                     /locus_tag="VEL68_07230"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ35792.1"
                     /translation="PMLVQGSKFSYKADSLILAVGERADLSSLPPEMKTENGLIAVDS
                     WGRTNLPGFFAGGDAATGAGYVSQAIASGKRAALAIEGYLRGKSGNPGEDRREVVKID
                     KINLDYFPTLSRVKGSSLAVKDRGKGLKEVHGGLLPARAKKEAERCFSCGNCIQCNVC
                     LMVCPDVAISYQERDGKFSIDYEHCKGCGICAVECPRSAMTLEEEKWNE"
     gene            619..1773
                     /locus_tag="VEL68_07235"
     CDS             619..1773
                     /locus_tag="VEL68_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ35793.1"
                     /translation="MERVISGNEAVAYGAMLSRVQVVSAYPISPQTTIVETLSDFCAS
                     GRLKADFVKVESEHSAMACIVGAASAGARAFTATSAQGLALMHEVLHWAAGSRLPIVI
                     VNVNRAMAAPWTMWCDQSDSLAQRDTGWLQFYCEDNQEVLDSIIMAYRISEKVLLPAM
                     VCLDGFILSHTYEQVEIPGIEEVDRYLPSYVAKLKLDVDDPHSFHAAMPPDIYMEQRV
                     KFQEAMEEAKGVAKEADEEFGRAFGRRYGIMEEYSAEDADILLVTSGTITGTARGVVD
                     DYRKKGEKVGLLKMKMFRPFPTEDIRRVLQKAKKVAVVDRNISFGSTGIFAQEIRSVL
                     HRHGEGTSVFGFIAGLGGRDVTPSVLQQIVEITKGKEAPSGDVVWIGAKP"
     gene            1770..2702
                     /locus_tag="VEL68_07240"
     CDS             1770..2702
                     /locus_tag="VEL68_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ35794.1"
                     /translation="MRYELPKEEFLYSGHIGCPGCGASLAMRYTLQVLGKKTMVVIPA
                     CCWSTVAGFFPSSSLKVPILHCLFETAAISASGVKKGLIARGIEDVQVMAWAGDGGTF
                     DIGLQSLSGCAERNDDIIYVCYDNEAYMNTGIQRSSATPLMSWTSTTPNPAFKETPKK
                     DIMEIMAAHRIPYAATVSPAFPEDFLKKMGRAKETRGFRFIHILSSCPPGWKFPSENS
                     LKVSRLAVETKIFPLYEIENGRKYALTHEPKGLPVGEYLKLQGRFSHLAPEQFQAIQE
                     NVDEEWTILKAKARGPKQRGNGKRQKEIKARRAR"
     gene            2816..3046
                     /locus_tag="VEL68_07245"
     CDS             2816..3046
                     /locus_tag="VEL68_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35795.1"
                     /translation="MASLMKWLILGWSLVVVLMWAFLAFRQRFSEQAIFYTSEDVWGF
                     MGVVFLTWLIPIAVFAFFAFLTHSSKSKKEEG"
     gene            3105..4379
                     /locus_tag="VEL68_07250"
     CDS             3105..4379
                     /locus_tag="VEL68_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35796.1"
                     /translation="MTPAAGPTAVSPTVFLFAICSARIFLYFCLQNYIALIPVLQIEW
                     HMSYTAAGSVVSAYQTGFLISMVSLAALSDWVSPKKVFLYSCIAFSVSSLLFAIFARS
                     YLSAFLLRGLMGLSLGGTYTPALKLISENFSSALRGRAMGFFIGAGSLGHASSLAATG
                     WLVARYDWQTTFFVTSLGPILGAVIPLWILWGLAERKPEPEGKMLKKEVLANRPALLM
                     IAGYSVHTWELEAQRAWTPAFLVACYLATGLTKDQALQSGAVFSSVMYVMGVVSTSAA
                     GYWSDRWGRTAVIVAMMAISISCSFSFGWLIGSPILWVMMVGLAYGFAVIAESPVYSS
                     GLSELVSPNFLGTALALRSLIGFGIGAVAPTAFGLVLDLTNSGETQKLLGYLPNWGWA
                     FSMLGLFALIGPWAMFKLRSMPESRMMAGGKK"
     gene            4743..5039
                     /locus_tag="VEL68_07255"
     CDS             4743..5039
                     /locus_tag="VEL68_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HXZ35797.1"
                     /translation="MAAKSKAVVINEKDNVATAVKPMKTGSTVSLEVQGRVEKIKLLS
                     DIPMGHKFALRGIDQGADVIKYGEPIGRTTAAIAKGEHVHVHNVVSHRGRKEGA"
     gene            5039..6196
                     /locus_tag="VEL68_07260"
     CDS             5039..6196
                     /locus_tag="VEL68_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HXZ35798.1"
                     /translation="MEFQGYRRPDGKAGVRNHVLVIPSVVCSQGAAEMISRNMKGAVY
                     LPNVFGCAQVGEDRAQTKRTLVGFGLNPNIFSVLVVGNGCENLPAKEIAEALAPAKKR
                     VEFIEIQEVGGTKKTAAMGRKILREMLADAAKLQRQPIPVSELIFATECGGSDYTSGL
                     GSNPALGACSDLIIEGGGTVILSETTELIGAEHLLAKRARTPEIGKQVLDLIAWWEKE
                     AIATGQDIRGANPAPGNIAGGITTIEEKSLGCIYKGGTKTLEEVIKYAFPPTKKGLIL
                     MDTPGHDIDQLTGMMAGGSQIAVFTTGRGTPTGSPIAPVIKITANDETYKKMKDNIDI
                     NVSSVVQGKETVKEAGKRIFDEVIAVASGKLCKAEKLGQRDFCIFKIGINF"
     gene            6284..7504
                     /locus_tag="VEL68_07265"
     CDS             6284..7504
                     /locus_tag="VEL68_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35799.1"
                     /translation="MRRKSLWMLVGAFSFVLGFSVPGWAATDVEIALFVCLSGPAADL
                     GTMSRDGAILAAEDVNQAGGIKALGGAKFKLVVADVTSNVAQGPSVVERTLSTQKISG
                     SIGFGMSQMTLSCLPVLEKAQVPLTTSSISDTITSQGYKYVFQIAPKGSHFGASQVKF
                     LTYLKNKYKFPINKVGFVYENTAYGTSTAGGLKATAEKEGYKIGLFEAYDAKFTDASP
                     LVNKIKASGVEVIFPVSYTTDAELIISTMQAMRVNPMVIGGGAGFIWPDIYKSLGDKI
                     TGVFSVGSWSWDSKNIMSDAYRKSVVERYRKRFGTFMPEQAGEHYAMVWTLKDALEAA
                     KSTDSKKVREALATMEITSGPAALMQPGKIKFDETGWNKYVYPTMIQWQKGDPRTVYP
                     EEAATNEVVWPIKR"
     gene            7625..8500
                     /locus_tag="VEL68_07270"
     CDS             7625..8500
                     /locus_tag="VEL68_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35800.1"
                     /translation="MDVEIVLQAIVNGLMMGGVYALIAVGLTLIFGVMNVVNFAHGEF
                     VMLGMVMTYLLHLFWGMDPGVSILLVIPVMLGVGALIQKGLISRVIGQPDEAQILLTL
                     GLSVLLINIALLFLGPDYRSVKTSYKFSVWAVGPILFSVPRLLAFVVAMAFSGILWLF
                     LTRTDLGKGLRAVAEKPEVATLMGINPKKMHALAFGIGIALAAGAGSMLTPFFYTFPS
                     VGGMFVLIAFVVVVLGGMGNIPGAILGALAIGVTESLTSQFVALDLAMLGVFIIFVLV
                     LVFKPSGILGKGKVY"
     gene            8500..9483
                     /locus_tag="VEL68_07275"
     CDS             8500..9483
                     /locus_tag="VEL68_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35801.1"
                     /translation="MSTSPQEHTRWTRYAPWILLALILIFPFIFPSRYLQRTMVNIFF
                     FAALGCAWNIIGGYAGQLSLGHAAFMAMGAYPTALLLLGLNVPPWLGFIPGVVVAVII
                     AFLLGHMVFGLRGPYLALSTLAFGEIVRILLLHFRDQTGGSLGLTIPFKGTDAWKLQF
                     ASEQPYYFICLALLGLCLYVTYKVHGSRMGYYLQAIKENQDAAENLGIDLANNKVKAL
                     MISAALTAVLGGFYVAYFYYIDPDSVCGMDLSIKILLVAIIGGVGTVWGPIIGACILI
                     PLTEISNYLFGSGRAGASLIIYAIILMIVVLFSPRGLIALLPGGERSYRHE"
     gene            9538..10293
                     /locus_tag="VEL68_07280"
     CDS             9538..10293
                     /locus_tag="VEL68_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35802.1"
                     /translation="MTNQGESANGVLLELQRVTKSFGGLTAVDNVSFSVRRGEIFGLI
                     GPNGAGKSTIVSLVMGIYQPDSGEVFFQGKSLLKLKANQVVERGICRTFQVEKPLLNM
                     TVTGNVLIGALLRTADPKEALEQSRVIIERVGLGKVGHVLAKNLTVQDRKRLELARAL
                     ATRPTMLLLDEVMAGLTPVEIDHSLDLLRKLRGEGLTILIIEHVMRAIMNISDRIVVL
                     DYGKKIAEGGPKEIAHNAAVVEAYLGKKGVRNG"
     gene            10286..10999
                     /locus_tag="VEL68_07285"
     CDS             10286..10999
                     /locus_tag="VEL68_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35803.1"
                     /translation="MGELLKVENLGVAYGDLQVLHDIEIRVDTGEIVAVLGSNGAGKT
                     TLLQAISGLLPIHQGKVFFENRDLASVPAYELPSLGMAHVPQGRGIFTTLTVMENLTI
                     GAWNPRGKKERERNLNQVFDLFPRLKERTSQMAATLSGGEQQMLAIGRALMQNPLLLM
                     LDEPSLGLAPILVEAVFATVEKIIAQGLSILLVEQNIFYAIDLAGRCYLLENGKINLE
                     GPRVQFMENPRIKEAYLGM"
     gene            11331..12443
                     /locus_tag="VEL68_07290"
     CDS             11331..12443
                     /locus_tag="VEL68_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ35804.1"
                     /translation="MKIADVRSYTLSVPLAKPWKIAGIVMAEMTATIVEVEADNGLVG
                     YGEALTRLGPSAAREVIQNILKPVVLGADPGNVDVLWEKMFSTMKGRGHWKGFMIESI
                     SGVDIALWDLIGKAMNHPVSHLLGGRHHEKLEAYASSIMLMERSEMVKEAKELVRKGF
                     KKIKVKVGLGVDTDFGNIKAIREAVGPDIGIMLDANCGFSAEEALRLGVMLEPLNILW
                     FEEPVPPQDLEGYARLSDSLRIPVVGGESEFTRWGFRDLILRGKVPMIQPDIARCGGF
                     TEARKIAAFASACGVTIAPHTGASGAVSIAAAVQWASSLSNLVIFEHMYTENPLRTEI
                     LRDPVLECHDSVIKVPGGQGLGIEIDKNKMKKYMKP"
     gene            12644..13447
                     /locus_tag="VEL68_07295"
     CDS             12644..13447
                     /locus_tag="VEL68_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXZ35805.1"
                     /translation="MRMRKFFVLLSIFSILTMISFAQGIAGTLQDAKARGKLLAGVKT
                     DFPPFGFVDEKGVNKGFDVDIAKALAKELFGKEDAVEFVAVTSGNRIAFLTTNKIDII
                     LASMTITEERKKVIDYSIPYFMSGHLILVQKDSKIEKYPDLAGKKVSTTQGSTGDIVI
                     GELVPNAERIKFQHNSESLQALKDRRVDAFVQDDVLILDLQKRNPELKIVGWPPFRPA
                     PYGLGVRKGDKEWLDFVDAALNKMKKSGEYQKLWDKWFGENKNVLLVLK"
     gene            13646..14308
                     /locus_tag="VEL68_07300"
     CDS             13646..14308
                     /locus_tag="VEL68_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXZ35806.1"
                     /translation="MAYQFDFSIIIKYLPLFLKGVFLTVEISFLAILIGLPVGILAAL
                     ARTSGFRILNLMGAVYVEVFRNTPLLIQIFIIFFGLPGIGIKLSPYISGLTALVLYVG
                     AYNTEVIRAGLEAVPRGQIEAAESLGLTGVQTFLHIILPQTFRISLPALGNNWVALVK
                     NSSLVSVIGMVELTWVTFDLNALTFRSFELFGAATIFYLILIFILINIQAFVEKRYLY
                     KT"
     gene            14317..14970
                     /locus_tag="VEL68_07305"
     CDS             14317..14970
                     /locus_tag="VEL68_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXZ35807.1"
                     /translation="MFDFDVIIKNLPYLLSGVKFTLALACLVIILGTFLGMILGLSKL
                     SKNLLVRYPANLIVEITRDTPIIMQMFFIFFGLPALGIRLATFPSAAIAMSLFAAGNT
                     AEIVRGAIDSLPTGQFDAAKSLGMNYLQMMIYVIIPQALRRMIPPAMGLFTTLIKDTS
                     LAAIIGAFELTRAGQEIIERTFHSLEIYLVVAAIYFIICYPLSYYTRKVEKQRVTGF"
     gene            14978..15703
                     /locus_tag="VEL68_07310"
     CDS             14978..15703
                     /locus_tag="VEL68_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXZ35808.1"
                     /translation="MIEARDIYKSFGKVQALNGVTTTIQKAEVVVIIGPSGSGKSTFL
                     RCLNHLEKIDRGSIIIDGIPLTDSKTNIHKVREEIGMVFQQFNLFPHLSVLRNITLAQ
                     IKVRKRSSEQAERIARDLLRKVGIPEKADNYPFQLSGGQQQRVAIARALAMQPRIMLF
                     DEPTSALDPEMIGEVLEVMKNLAKEGMTMVVVSHEMGFAKEVADRVIFMDEGKIVEEG
                     TPDRLFDHAREERTRTFLSRVLK"
     gene            complement(15796..15894)
                     /locus_tag="VEL68_07315"
     CDS             complement(15796..15894)
                     /locus_tag="VEL68_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 family protein"
                     /protein_id="HXZ35809.1"
                     /translation="MSRSAISGDPEDTDVYGSQQHVPLMGIELPRE"
BASE COUNT         3543 a         4239 c         4555 g         3567 t
ORIGIN      
        1 tcccatgctg gtccaggggt ccaaattttc ctataaggcc gattctctga ttctagccgt
       61 gggcgagagg gcggacctct cctccctgcc tccagagatg aaaaccgaaa acggtctgat
      121 cgccgtcgat tcctggggtc ggaccaacct ccccggattc tttgccggtg gcgatgcggc
      181 cacaggggca ggttatgttt ctcaggccat cgcctccgga aaaagagcag ccctggccat
      241 agaaggttac ttacggggca agagcggaaa cccgggagag gaccgcagag aagtggtcaa
      301 gattgacaag atcaacttgg attattttcc gaccctgagc cgggtgaagg gctcttccct
      361 ggcagtgaaa gaccggggaa aggggctcaa ggaggttcat ggagggcttt tgcctgcccg
      421 ggccaaaaag gaggccgaac gctgtttcag ctgcgggaac tgcatccagt gcaatgtttg
      481 cctgatggtt tgtccggacg tagccatttc ctaccaggaa agagacggaa aattttccat
      541 cgattatgag cactgcaagg gctgcggaat atgcgcggta gaatgtcccc gctcagccat
      601 gaccttggag gaagagaaat ggaacgagta atttcgggca atgaagccgt ggcctatgga
      661 gccatgcttt cccgggttca ggtcgtatcg gcctatccca tatcccctca gaccaccatc
      721 gtggaaacgc tctccgattt ctgcgccagt ggccggctga aagcggattt cgtcaaggtc
      781 gaatcggagc actcagccat ggcctgcatc gtcggggccg cctccgccgg ggcgagggcg
      841 tttaccgcca cgtccgccca gggcctggcc ctgatgcatg aggttctgca ttgggcggcc
      901 ggttcccgtc tgcccatcgt catcgtcaat gtcaatcggg ccatggccgc tccctggacc
      961 atgtggtgcg accagagcga tagcctggcc cagagggaca ccgggtggct gcagttttac
     1021 tgtgaagaca accaggaagt cctggacagt atcatcatgg cttaccgcat ctccgaaaaa
     1081 gtcctcctgc cggccatggt ctgcctggat ggcttcatcc tttcccacac ctacgaacag
     1141 gtggagattc cagggatcga agaggtggac cggtacctgc cttcctacgt ggccaaacta
     1201 aagctggacg tggatgaccc ccattctttc catgcggcca tgcctcccga tatttacatg
     1261 gagcagcggg tgaaattcca ggaagcgatg gaagaagcca aaggcgtggc caaggaggcg
     1321 gacgaagaat tcggcagggc cttcggccgg cggtacggta tcatggagga atactctgcc
     1381 gaagatgcgg acatcctcct ggtcaccagc gggaccatca ccggaaccgc ccggggggtc
     1441 gtcgatgatt accgtaagaa gggggagaag gtcggactcc tgaaaatgaa aatgttccgg
     1501 ccctttccca ccgaggatat tcgaagagtt ttgcagaagg cgaaaaaagt ggcagtcgtg
     1561 gaccggaaca tctcttttgg gtccaccggg atctttgccc aggaaatccg gtcggtattg
     1621 caccggcacg gcgaggggac ctcggtgttt ggattcatcg ccggactggg gggaagggac
     1681 gtgacccctt ccgttcttca acagatcgtg gagattacca aaggcaaaga ggccccgtca
     1741 ggagacgtgg tctggatagg agccaagcca tgaggtacga acttcctaaa gaagagtttc
     1801 tttattcagg gcatatcggg tgtccgggct gcggtgcgtc cttggccatg cggtataccc
     1861 tgcaagtcct gggcaagaaa accatggtgg tgattccggc ctgctgctgg tcgactgtgg
     1921 caggattctt tccttcctcg agcctcaagg tgccgatcct ccattgtctc ttcgagactg
     1981 cggccattag cgcctccggg gtgaagaagg gtctgatcgc ccggggaatc gaggacgttc
     2041 aagtgatggc ctgggccggg gacgggggaa cttttgacat cggcctgcag tctctttcgg
     2101 gttgtgccga gaggaatgat gacattatct acgtatgtta cgacaacgag gcctacatga
     2161 acacggggat tcagaggagc tcggccacgc ccctcatgag ctggaccagc acaaccccca
     2221 acccggcttt caaagagact cccaaaaaag atatcatgga gatcatggcc gcccaccgga
     2281 tcccctatgc cgccacggtc tccccggcct ttcccgagga ctttttgaaa aagatgggca
     2341 gagccaagga gacccggggg tttcgtttca tccatattct ctcctcttgc cctcctggat
     2401 ggaaattccc ctcggaaaat tctctgaagg tctcccggct ggcggtggag acgaaaatat
     2461 ttccccttta tgaaatcgag aatggaagaa agtacgccct cacccacgag cccaagggct
     2521 tgccggtcgg cgagtacctg aaactgcaag ggaggttcag tcatcttgcc ccagagcaat
     2581 tccaggccat tcaagagaat gtggatgaag aatggaccat tctaaaagca aaggcccgag
     2641 gtcccaagca aaggggaaac gggaaaaggc aaaaggagat aaaggcgcga cgagcgaggt
     2701 gaaaaataaa gaacaatagc caaaattaat aggcacaagt tccgggggag cagggaatac
     2761 cgatgataag ggttttttct aaggcctcgg acttcgtggc ccggacctgt actcaatggc
     2821 ttcgttgatg aaatggctga tcctgggctg gtccctggtg gtggtcctga tgtgggcttt
     2881 tctggctttc cgccagcgct tctcggaaca agccatcttt tacacctccg aagacgtgtg
     2941 gggttttatg ggggtcgtat tcctcacctg gctcatcccc atcgccgtct tcgctttttt
     3001 cgcgtttctg acccactctt caaaatccaa gaaagaagaa ggatgattcc tcttctccgt
     3061 cggaatccaa ccaggcattc cacccggata gaaacggaaa aggaatgaca cccgctgcag
     3121 gcccgacggc cgtctccccc accgtttttc tattcgccat ctgctcggcc cggatttttc
     3181 tttacttctg ccttcaaaac tatatcgctt tgattccggt cctgcagatt gaatggcata
     3241 tgtcctatac tgcagcaggg tccgtcgttt ccgcctacca aacgggtttt ctgatctcca
     3301 tggtcagcct ggccgctcta agcgattggg tgagccccaa gaaggttttt ctctattctt
     3361 gtatcgcctt ttccgtttcc tccctcctct tcgccatttt tgcccgttcc tacctttcgg
     3421 cttttcttct ccggggactc atgggattgt ccctgggcgg aacctatacc ccggctttaa
     3481 aattgatttc cgaaaacttt tccagcgccc tccgcggaag ggccatgggc tttttcatcg
     3541 gggcgggttc cctcgggcac gccagctcgc tggccgccac cgggtggctg gtggcgcgat
     3601 acgattggca gaccactttt ttcgtgactt cgctggggcc gatcctggga gcggtcattc
     3661 ccctttggat tctgtggggg ctggcagaaa ggaaacccga accggagggg aaaatgctga
     3721 agaaggaggt cctggccaac cgcccggccc tgctgatgat tgccggctac agcgtccaca
     3781 cctgggagct cgaagcgcag cgtgcctgga cgcccgcctt tctggttgcc tgttacctgg
     3841 caacgggatt gaccaaggac caggccctcc agtcgggggc ggtcttctct tcggtcatgt
     3901 atgtcatggg ggttgtatcc acctcggccg cgggctactg gtcggaccgc tggggccgca
     3961 cggcggtgat cgtcgccatg atggccatca gcatttcctg ctccttctcc ttcggctggc
     4021 tgatcggcag cccgatcctg tgggtgatga tggtggggct ggcttacggt tttgcagtga
     4081 tcgccgaatc cccggtttat tcctccggac tttcggaact cgtctccccc aacttcctgg
     4141 ggaccgccct ggcccttcgg tccctgatcg gcttcgggat cggcgccgtg gctccaactg
     4201 cgtttgggct ggtcctggac ctcaccaact cgggggaaac gcagaaactt ctcggttacc
     4261 tcccgaactg gggctgggcc ttcagcatgc tgggcctgtt cgctttgatc gggccctggg
     4321 cgatgtttaa actccgatcc atgccggaga gccggatgat ggcagggggg aagaagtaga
     4381 gaatgacgcg aggaattccg gcggacctct tcttctcccg ccggatttgg ccggcccgca
     4441 tgggacccgc ccctaatccc cgacacaagc gggtgaacca tgtaaaggcg ggtttcaaac
     4501 ccgtccctac aacacgcgaa gtgccaaaac ttttataagg acatttctga acagggttgt
     4561 gtagggacgg ccctctgtgg ccgcccacaa gaggggcaac cacggggggt tgcccctaca
     4621 aggttcaaag gaaaggccgt gcaggaacgg actgaaaaga agttgcaaaa atttccagtt
     4681 ggggtatttt gaatatattt ttgatgggga aaacttccga cgccccatga aggaggttgg
     4741 aaatggcagc caagtcaaag gcagtcgtca tcaatgaaaa ggacaatgtg gccaccgccg
     4801 tcaagcccat gaagaccggc agcacggtct ctctcgaagt gcaggggcgg gtggaaaaga
     4861 tcaagctcct gtcggacatt cctatgggcc ataaatttgc cctgcggggg atcgatcagg
     4921 gtgcggatgt gatcaagtat ggcgaaccca tcggcaggac caccgcagcg attgccaaag
     4981 gggaacacgt tcatgtccac aacgtcgtca gccaccgggg aagaaaggaa ggtgcctgat
     5041 ggaattccag ggataccgga gaccggatgg gaaagccggg gtaaggaacc acgtgctggt
     5101 gatccccagc gtggtctgct cccagggagc ggcggagatg atctcccgga acatgaaagg
     5161 ggccgtctat ctgcccaatg ttttcggctg cgcccaggtg ggtgaggacc gggcccagac
     5221 caaacggacc ctggtgggat tcggccttaa tccaaatatc ttttccgtgc tggtggttgg
     5281 aaacggctgc gagaatctgc ccgccaagga gattgctgaa gccctggccc ccgccaaaaa
     5341 gcgggtggaa ttcattgaaa tccaggaagt ggggggcacc aagaaaaccg ccgccatggg
     5401 gagaaaaatc cttcgggaga tgctggccga tgcggcgaag ctccagcggc agccgattcc
     5461 cgtctcggaa ctgatcttcg ccaccgaatg cggggggtcg gattatacct ccggcctggg
     5521 ttccaaccca gccctggggg cgtgcagtga tttgatcatc gaaggcggcg ggacggtgat
     5581 tctttcggaa accaccgagt tgatcggggc ggaacatctt ctggccaaga gggctcggac
     5641 tccggagatc ggcaaacagg tcctggacct catcgcctgg tgggaaaaag aggccatcgc
     5701 caccggccag gacattcgcg gcgcgaaccc agccccggga aatatcgccg gcggtatcac
     5761 caccatcgag gagaaatccc tgggctgcat ctacaaaggg ggaacgaaga cactggagga
     5821 agtgatcaag tatgcttttc cccccacgaa aaagggactg attctcatgg acacccccgg
     5881 ccacgacatc gaccagctta ccggcatgat ggccggaggg tcgcagatcg cggtctttac
     5941 caccggaagg ggaaccccca ccggatcgcc cattgctccg gtcatcaaga tcacggccaa
     6001 tgacgagacc tacaagaaaa tgaaggacaa catcgacatc aacgtgagct cggtcgtcca
     6061 gggaaaggag accgtgaagg aggcgggcaa gcggatcttc gacgaggtga tcgccgtagc
     6121 ttccggcaag ctctgcaagg ctgagaagct gggacagcgg gatttctgca tcttcaagat
     6181 cggaatcaat ttttagctga ccggttattt tttttattca gagcggcgaa ttttgcctgt
     6241 ttgtggaaat aatgacgtcc agcataagaa aaggaggtag acaatgcggc gaaaatcttt
     6301 atggatgttg gtaggggcat tttctttcgt gctaggtttc tccgttcctg gatgggccgc
     6361 cactgatgtg gaaatcgccc tgtttgtatg cctttcgggg ccggctgccg acctggggac
     6421 catgagccgg gacggggcca ttctggccgc ggaagacgtc aatcaggcgg gcggaatcaa
     6481 ggccctggga ggcgccaagt tcaagctggt ggtggccgac gtgaccagca acgtggctca
     6541 gggccctagt gtggtcgagc ggacgctttc cacccagaag atttcgggct ccatcggttt
     6601 cgggatgagc cagatgacgc tcagctgcct gccggttttg gaaaaggccc aggtccccct
     6661 gacgaccagt tcgatctcgg acacgatcac ctctcagggc tacaagtacg tattccagat
     6721 tgctcccaag ggaagccatt tcggcgctag tcaggtgaaa tttttgacgt accttaagaa
     6781 taaatataag ttccccatca acaaagtagg attcgtttat gaaaacacgg cttacgggac
     6841 ttcgaccgcc ggtgggctga aggcgaccgc ggagaaagaa ggctacaaaa ttggtctgtt
     6901 cgaggcttac gacgcaaaat ttaccgacgc ctctcccctg gtcaacaaga tcaaagcttc
     6961 gggggtggag gtgatctttc cagtctccta cacgacggat gccgagctga tcatcagcac
     7021 catgcaggcc atgcgggtaa atcccatggt gatcggcggc ggggccggat tcatctggcc
     7081 ggatatttac aaatccctgg gcgacaagat cactggagtc tttagcgtgg ggtcctggag
     7141 ctgggatagc aagaacatca tgtccgacgc ctatcggaaa tcggtggtcg aacggtaccg
     7201 aaagcggttc gggaccttca tgccggaaca ggccggggag cactacgcca tggtctggac
     7261 gctgaaagac gccctggaag cggccaaatc cacggactcc aaaaaagttc gggaagccct
     7321 ggcgactatg gaaattacca gcggccccgc cgctctgatg cagccgggga agatcaagtt
     7381 tgacgaaacc ggctggaaca agtatgtcta tcccaccatg atccagtggc agaagggcga
     7441 tccgcggacg gtgtaccccg aagaggcggc caccaacgaa gtggtgtggc cgatcaagag
     7501 gtagcttgcc ccccgctttt cattcagccg aattgtccac ccccctccct ctccttcaaa
     7561 aggatgagtg aggggggtct aggcgcacga acttgtgaaa tgatctaaac cggggccgga
     7621 aaaaatggat gtagaaatcg tattgcaggc catcgtgaat gggctcatga tgggtggcgt
     7681 ttacgccctg atcgccgtcg ggttgaccct gattttcgga gtgatgaacg tcgtgaattt
     7741 cgcccacggc gagtttgtca tgctggggat ggttatgacc tacctcctgc atcttttttg
     7801 ggggatggac ccgggtgtct ccatcctgct ggtcattccc gttatgctgg gtgtgggggc
     7861 gctgattcag aagggattga taagccgggt catcggccag cccgatgaag cccagatcct
     7921 cctgaccctg gggctttcgg tgctcctgat caacattgcc cttctctttc tcggccctga
     7981 ctaccggtca gtcaagacca gctacaagtt cagcgtctgg gcagtggggc cgatcctgtt
     8041 ctccgttccg cgcctgttgg cgtttgttgt ggccatggct ttctcgggaa tcctttggtt
     8101 gttcttgacc cggacggact tggggaaagg tctgcgggct gtggcggaaa aaccggaagt
     8161 ggccaccctc atggggatca accccaagaa aatgcatgcc ctggccttcg ggatcggcat
     8221 cgccctggct gccggggcgg gcagcatgct gacccctttc ttttacacct ttccctcggt
     8281 aggaggcatg ttcgtcctga tcgcctttgt cgtcgtggtc ctggggggaa tggggaacat
     8341 cccgggcgcc atcctcgggg ccttggccat cggggtcacc gaatcgttaa cctcccagtt
     8401 tgtggccctg gacctagcca tgctgggggt cttcatcatt tttgttctag tcctggtctt
     8461 caagccctcg ggcatcctcg gaaaagggaa ggtttattga tgagtacgtc accccaagaa
     8521 catacccgat ggacgcgtta cgctccgtgg attttgctgg ctctcatcct catttttccc
     8581 ttcatctttc cttcgcgcta cctgcagagg acgatggtca acattttctt ctttgccgca
     8641 ctgggttgcg cctggaatat catcggggga tacgccgggc agctgtcctt ggggcacgcc
     8701 gccttcatgg ccatgggcgc atatccgacg gcgctcttgc tcctgggtct caatgtcccg
     8761 ccctggctgg gattcatccc cggagtggtt gtggcggtca tcatagcttt tctcctgggc
     8821 cacatggttt tcgggcttcg cggcccttat ctggctcttt ccaccctggc cttcggggag
     8881 atcgttcgaa tcctgcttct ccattttcgc gatcaaaccg gcggatccct gggattgacc
     8941 attcctttca aggggaccga cgcatggaag ctgcagttcg cctccgaaca gccctattac
     9001 tttatatgcc tcgctttgct gggactttgc ctctatgtga cctacaaggt tcacggttcg
     9061 cggatgggct attacctcca ggcaatcaag gaaaaccagg acgcggcgga aaatttggga
     9121 atcgacctgg ccaataataa agtcaaggcc ctcatgatca gcgcagcctt aaccgctgtg
     9181 ctgggcggat tctacgtggc ctacttctat tacatcgacc cggatagcgt atgcggcatg
     9241 gacctttcca tcaagatcct cttggtggcc atcattggcg gggttggaac cgtgtgggga
     9301 ccgatcatcg gggcctgcat cctgatcccc ctgaccgaaa tcagcaatta cctcttcggt
     9361 agcggcaggg cgggggcgtc actgattatt tacgccatca tcctcatgat cgtagtcctc
     9421 ttcagcccca ggggcctaat cgcccttctg ccgggaggag agcgaagtta tcgccatgaa
     9481 tgattcgagc ttattcatcg tatgcctttc tgattaatcc aagaaaagga gcccaaggtg
     9541 acgaaccaag gggagtctgc taacggggta ctgttagagc tgcagcgggt caccaaatcc
     9601 tttgggggcc tgaccgccgt ggacaatgtc tccttctctg tccgccgcgg cgagattttc
     9661 gggttgatcg gcccaaacgg ggctggcaag agcacgatcg tcagcctggt catggggatc
     9721 taccagcccg attcgggaga ggtctttttc cagggaaaaa gtctactgaa actcaaagcc
     9781 aaccaggtgg tggagagggg gatctgccgc acctttcagg tggaaaagcc tctcctgaat
     9841 atgacggtta ccgggaatgt cctgatcgga gcgctgctcc ggacggcgga ccccaaggaa
     9901 gctctggaac aatccagggt gatcatcgag cgagtgggcc tgggaaaagt ggggcatgtg
     9961 ctggccaaga acctcacggt ccaggatcga aaacgcctgg aactggcccg cgccctggcg
    10021 acccgcccta cgatgcttct gctggacgag gttatggccg gcctgacccc tgtggaaatt
    10081 gatcactccc ttgatttgct ccgcaaactg cggggggagg gcctgaccat tctcatcatc
    10141 gaacatgtga tgcgggccat catgaacatc tccgaccgaa ttgtggtcct ggattacggc
    10201 aaaaagatcg ccgagggagg gccgaaggag atagcccaca atgcggcggt ggtggaggcc
    10261 tacctgggga aaaaaggagt tcggaatggg tgaattgctg aaagtggaaa atctgggggt
    10321 cgcctacggg gatctgcagg ttctccatga catcgagatc cgggtcgaca ccggagaaat
    10381 cgtcgctgtc ctgggttcca atggggctgg caaaacgacc ctgctccagg ctatttcggg
    10441 gctcttaccc atccatcagg gaaaggtttt ctttgagaac cgggatctgg ccagtgtccc
    10501 ggcttatgaa ctcccgagct taggaatggc ccatgttccc cagggaaggg ggattttcac
    10561 taccctgacg gtcatggaga atctcaccat cggggcctgg aatccgcgcg gaaagaagga
    10621 acgcgagagg aatctcaacc aggtttttga tcttttcccg aggctcaaag aacgcaccag
    10681 ccagatggcg gcaaccctct cggggggaga acaacagatg ctggccatcg ggcgcgccct
    10741 gatgcagaat cctcttctgc tgatgctcga cgaaccttcc ctcggcctgg cccccatcct
    10801 ggtggaagca gtctttgcga cggtggaaaa gatcattgcc caggggcttt ccatcctgct
    10861 ggtggaacag aacatttttt acgccatcga tctggccggt cgctgttacc tgctcgaaaa
    10921 cggaaaaatc aacctggaag gacctcgtgt ccagttcatg gaaaatccca ggatcaaaga
    10981 ggcctacctg ggaatgtaac tcaaatagga agcgccaaat tcaaagcatc aaataacaaa
    11041 caaaccccaa aggacaaatt ccaaacgaaa tctaatcctt aacaggatgc tgaaaaattc
    11101 ctttgcggcc gttggtcgtt attcctcgcg aaagcgggaa tctagtcttt tcaaggtatt
    11161 ctggactccc gcttccgcgg gagtgacggt ggatggggtt ttttcagcgg cccgctaaaa
    11221 tccaaacttc atacattgaa aaattataga ttggaattta ttgggggttt gcatattggg
    11281 atttcccccc caagggggga catgagaatt ttctggaagg aagtgaattt atgaaaattg
    11341 cagatgtaag gtcctacacc ttgtccgttc cactggcaaa accctggaag atcgcgggga
    11401 tcgtgatggc cgagatgact gccaccatcg tggaggtgga ggccgataac gggcttgtcg
    11461 gttacgggga agccctaacc cgcctgggcc catcggcggc cagggaggtg attcagaata
    11521 tcctaaagcc cgtggtcctg ggggctgacc ccggcaacgt agacgtgctc tgggagaaga
    11581 tgttctcaac catgaagggc cgggggcact ggaaggggtt catgatcgag tccatcagcg
    11641 gggtggacat cgccctctgg gacctgatcg ggaaggccat gaaccatccc gtttcccatc
    11701 ttctcggggg ccggcatcat gagaagctgg aagcctatgc ctcttccatc atgctcatgg
    11761 agcgctccga gatggtgaag gaggccaaag agctggtccg gaaggggttc aaaaagatca
    11821 aagtgaaggt aggcctgggt gtggataccg acttcggcaa catcaaggcc attcgcgaag
    11881 ccgtaggccc ggacatcgga atcatgctcg acgccaactg cggcttcagt gcggaggagg
    11941 ccctacggtt gggggtgatg ctggaaccgc tgaacatcct ctggttcgaa gaacccgtgc
    12001 cgccgcaaga cctggaagga tatgcccggc tctccgattc cctacgaatc ccggtggtag
    12061 ggggagagtc ggagttcacc cgctggggct tccgcgatct catcctgcgg ggaaaagtcc
    12121 ccatgatcca gcccgacatc gcccgctgtg gcggcttcac cgaggcccgc aagatcgccg
    12181 ccttcgccag cgcctgcggg gtgaccatcg cccctcacac cggggcatcc ggcgcggtgt
    12241 ccattgctgc cgccgtgcag tgggcttcgt ccctttccaa tctggttatt tttgaacaca
    12301 tgtacacgga gaaccccctc cggacagaaa tcctgaggga cccggtgctc gaatgccacg
    12361 attcagtaat caaagtccca ggcgggcaag gcctggggat cgagatcgat aagaataaga
    12421 tgaaaaaata tatgaaaccg tagggagtaa ggtggacagc acaaggtata aacatcggat
    12481 gaaggattgc ggttcgagcc ccgtgggaga agggagcaaa gagtgatacc gcgggccagg
    12541 gccgagggtc ggaggaaaat cgattgccct tgacaaaaag ggatatctca tataaattcg
    12601 atttcgactt taaaaatccg tatttttgaa aggaggaatc tatatgagaa tgagaaaatt
    12661 tttcgtttta ttgtcaatct ttagtatctt aacgatgatt tcattcgccc agggaatcgc
    12721 aggaaccttg caggatgcca aagcaagagg gaaactgctc gcaggagtga agaccgattt
    12781 cccgcccttc ggttttgtgg atgagaaggg ggtgaataaa gggttcgatg tggacattgc
    12841 caaagcattg gccaaggagc tcttcgggaa ggaggatgcc gtagagttcg tggccgtcac
    12901 ctcgggaaac cgaatcgcct ttctaaccac gaataagatc gatatcatct tggccagcat
    12961 gaccatcacc gaggaacgaa aaaaggtgat cgattacagc atcccctatt ttatgtccgg
    13021 ccacctcatc ctggtccaaa aggatagcaa gattgagaaa tatccagacc tggcggggaa
    13081 gaaggtctcc accacccaag gctccacagg cgatatcgtc atcggggaac tggttcctaa
    13141 tgctgagcgg atcaaattcc agcataattc cgaatcgctc caggccctga aagaccgtcg
    13201 ggtagacgcc ttcgtacagg acgatgtcct cattctcgat cttcagaaga gaaatccaga
    13261 attgaaaatc gtgggctggc ctccgttccg tcccgctcct tatggactgg gggtgaggaa
    13321 aggagataaa gagtggctcg acttcgtgga tgccgcctta aataaaatga agaaaagcgg
    13381 cgaatatcaa aaactttggg acaaatggtt tggagagaat aagaacgtcc tcctagtttt
    13441 gaagtagtgg ccgagttacc gaaacatcat ggggttggag aggaggtcca accccatttt
    13501 cattgctaat aggtcgatgc caaataccca tctgcggcct tacgctcatc cttctttcat
    13561 tatggaagtc ctctctgcac ctcatttcta aggattcgat taccttgcat ctgggcattt
    13621 ttgcttcgct ttaaaaaaaa tctatatggc ctatcaattc gacttctcga ttatcatcaa
    13681 gtatcttccc ctattcctga agggggtctt cctcaccgtg gagatctcct tccttgctat
    13741 tcttatcgga ttgccagtcg gaatcctcgc ggcgctcgcc cggacttcgg gatttaggat
    13801 cctcaacctc atgggagccg tgtatgtgga ggtcttccgg aacacgccgc ttctcattca
    13861 gatcttcatc atcttctttg gacttcctgg cattgggatt aaactttctc cttatatctc
    13921 cggcctgacc gccctcgttc tctatgtagg agcttataac acggaagtca tccgtgccgg
    13981 cctggaagcg gttccacggg gacagattga ggcagccgag tccttggggc taaccggagt
    14041 tcaaaccttt ctccatatta ttcttcccca aactttccgg atctccttgc cggccctcgg
    14101 gaataattgg gtggctttag ttaagaattc gtccctggtg tcggtcatcg ggatggtgga
    14161 gctaacctgg gttacctttg acctgaatgc cctgactttc aggtcctttg aactctttgg
    14221 agcggcgacg atcttctatc tcattctcat ttttatcttg attaacatcc aggcttttgt
    14281 ggaaaagcgt tatctttata agacctaggc cagaatatgt tcgactttga cgtgattatt
    14341 aagaatctac cctatctcct gtccggtgta aaattcaccc tggcattggc ttgcctggtg
    14401 atcattttgg gaactttctt gggaatgatc ctgggcttat ccaagctttc caaaaatctt
    14461 ctggtccgat atccggccaa tctcattgtg gagatcaccc gcgatacccc gatcatcatg
    14521 cagatgttct tcatcttttt tggactcccg gcattgggaa ttcgtttggc gaccttccct
    14581 tcggcggcca ttgccatgag tctcttcgcc gcgggaaata ctgcggaaat cgtccggggg
    14641 gcgatcgact cccttcccac gggtcaattc gatgcggcca aatcattggg gatgaactac
    14701 ctccagatga tgatttatgt gatcattccg caggcccttc ggcggatgat ccccccggca
    14761 atgggtctct tcaccaccct cattaaagat acctcattgg cggccataat cggcgctttt
    14821 gaactgaccc gcgcgggaca ggaaatcatc gagaggacct tccattccct ggaaatctat
    14881 ctggtcgtcg cggccattta ttttatcatc tgctatcccc tgtcctacta cactcgaaag
    14941 gtggagaaac aaagagtcac gggcttctga ggtccttatg attgaagcga gagatatcta
    15001 caagagtttc ggaaaagtgc aagctctcaa tggggtaacg acaaccatcc aaaaggccga
    15061 ggtggtcgtc atcatcggcc cgagcggctc tggaaagtca acctttcttc ggtgtttgaa
    15121 tcatctggaa aagattgacc gggggtcgat catcattgac ggcatccccc tgaccgactc
    15181 caagaccaat attcacaagg tgagggagga gatcggtatg gtcttccagc aattcaatct
    15241 cttcccccac ctcagtgtgc ttcggaacat caccttggcc cagatcaagg taagaaagcg
    15301 ttcctctgag caagcggaac gaattgctcg ggacctcttg aggaaagtgg gaattccaga
    15361 aaaggctgac aactatcctt ttcagctttc tggagggcaa caacagcggg tagccattgc
    15421 cagggccttg gccatgcagc cccgaatcat gcttttcgac gaacccacct cggccttaga
    15481 cccagagatg atcggggagg tactggaggt catgaagaat ttggccaagg aagggatgac
    15541 catggtggta gtgagccatg agatgggttt tgccaaagag gtggccgacc gggtcatctt
    15601 tatggatgag ggaaagatcg tcgaagaagg aactccggat cgtctctttg accatgcccg
    15661 ggaggaaagg acacgaacct ttctaagcag agttttgaaa taatcccccc tttccgaaag
    15721 gaagaaaggg gggattagga tgaccgacca gtattgttgc cagatcgaca agcctctggg
    15781 taattcctcc gttgttcatt ccctaggaag ctcgatcccc atgaggggaa cgtgctgctg
    15841 ggagccgtac acatccgtgt cttccggatc cccgctgatg gccgaccggg agatagtgac
    15901 ttta
//
LOCUS       DASPRR010000189        15711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1821624, whole genome shotgun sequence.
ACCESSION   DASPRR010000189 DASPRR010000000
VERSION     DASPRR010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15711)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15711
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821624"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1586)
                     /locus_tag="VEL68_07320"
     CDS             complement(96..1586)
                     /locus_tag="VEL68_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HXZ35810.1"
                     /translation="MRFVIAMMKHETNTFSPVPTPLASFGRQGDIFGSKALGAYQGTN
                     TPMAAFIDLARKEGAEIVTPIAASAWPSGPVESAAYEEMSRAICESVARGCDALFLDL
                     HGAMVSQGTDDGEGTLLKRIRSIAPELPIAVALDLHTNLTGEMVAHCTAMAGYQTYPH
                     VDTYETGKKVGQIVIESLKGRIHPTMAWMSCPMIPHTLRMGTNEPPMGNLISLAQLEE
                     KNALAVSVFGGFPLADIYQAGLSVLAVTDGDRRRAGEICRKLAKEAWGRREEFLYRAE
                     PLEQSVARAKAIKEGPILLIDHADNCASGGTQDTMAVVAEVIRQGLEDVAVGAIRDPE
                     AVATMIKAGVGSTVTLPLGGKMDMPAIRRKGEPFELTGRVRTISDGRFIARGPMFTGV
                     TMTLGRAVVLDTGAMQVVVIEENHEPFDLGIFRSVGIEPTAKKFLLLKSRIHYRAGFL
                     PISKAVIECNGVGVTGSDYRQFPFAKLRRPIFPLDPNTAFDTSFDE"
     gene            complement(1615..2349)
                     /locus_tag="VEL68_07325"
     CDS             complement(1615..2349)
                     /locus_tag="VEL68_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35811.1"
                     /translation="MIALKNIHSYYGTSHVLQGINISIEQGQAVSLLGRNGAGKTTTL
                     KTIMGLVHPKEGSIRYKGEEICGLKPFRIARKGISYVPEERAIFSYLTVVENLEIARV
                     AKGKDHQGTWTLDRVFAEFPKLAERRQHRGAKLSGGEQQMLSIARALMNEPQLLLLDE
                     PSEGLAPIIVQELQRITQEILRENITVLLVEQNLAMCTALCSHHYIIDQGSIVYHGSN
                     EEFMANEEVKDRYLTLSAVEQAMEGD"
     gene            complement(2346..3089)
                     /locus_tag="VEL68_07330"
     CDS             complement(2346..3089)
                     /locus_tag="VEL68_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35812.1"
                     /translation="MILRTVNLTKNFGGLMAVDHVNMEVEEGAVHSIIGPNGAGKTTF
                     FNILTGEISPTEGEVFFQEKNITGLKPHQVAKMRIGRSFQLNSLFKNLTVLENLRLAA
                     HSQHPGHFNFLKDFHSYPSPVEQARKVLEELEMTPIVAKKAGELSHAGQRTLEVAIAL
                     AANPLLLLLDEPTSGMSPEETIRMIRLIKRLAKQRAMVVIEHNMQLVWSISTVITVLH
                     QGQVIATGTPSQIEKNEKVRKAYLGGAAE"
     gene            complement(3086..4081)
                     /locus_tag="VEL68_07335"
     CDS             complement(3086..4081)
                     /locus_tag="VEL68_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35813.1"
                     /translation="MIEKALGKRKSWIDGEIFILAGLLLLLPLLLPYKTLPAEIVIYA
                     LGAVSFNMLLGYTGLLSFGQAIFFGSGAYLMGLLLIHFGLHPILTLALSGILAALFAA
                     VIGFLSIQQVGIYFVMLTLAFNQLAFFMAYQWKGITGGDDGLLNVPRPDFSLFPGLEL
                     PIQGNFQFFLFVWMIFLLSFIVIRRIIHSPFGRVLMAIRENPDRTRALGYNVIHYKVM
                     AFTLSGFFTGVAGGLYALFIKMVPINAIELGTSTDFIIMSLVGGTGSLFGPIIGAIVV
                     KVASEILSVLWARWLFILGVIFVGFVLFMRGGIWGILSDSWNKFTATRAGQGGKS"
     gene            complement(4243..5112)
                     /locus_tag="VEL68_07340"
     CDS             complement(4243..5112)
                     /locus_tag="VEL68_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35814.1"
                     /translation="MELILVQLFNGISLGMIYVLMAVGLTVIFGMLNIINFAHGAVYA
                     LGAYLAFQTSVLLGEWSGSFWVAIFLSPVIAGLLGALLEVVLLRKMYDEEHVYQILLT
                     FGITLILQEVIIMIWGPVGKSVPMPKQLAGVVDLGTFSFPEYRIFLVVFIAAICLISW
                     LLIEKTSYGSIIRAGTENRNMVNSLGINIYTVFTVVFSIGMGLAALAGAVSLPMRGAM
                     PLMGDSVLTLCFVVVVIGGMGSFTGAILGGLIVGIVESMMALVWPPGAMIATFAAMAL
                     VILIRPRGLLGFR"
     gene            complement(5368..6558)
                     /locus_tag="VEL68_07345"
     CDS             complement(5368..6558)
                     /locus_tag="VEL68_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35815.1"
                     /translation="MKRIRLMTLAMTFMLALILPAGAAEVVEIGMVSTFSGAYAAIGD
                     HGLKGAKYAIGEVNNKILGKPAEAYAIEDEAKPGVGARKVREAIDSRGIKFYQGAVSS
                     AVALAVSQVANEKKAVYVCSVGADEITGKACNFYTFRWSLPTYGAIQQTIIPLIKKYP
                     EAKRWYTITPDYVFGHSLLNNAVEVFKQYGIQHVGNDMHPIGHTEFSSFFTKAMAAKS
                     DFVVFLNFGKDTINALKMADNFGLKKKAKLLVAWSSGLMDLQEIGPETAEGIYFGCQF
                     WHDVPVPATQSLTKAYQAKFQDIISYPAASSYIMTRMIILAIEKAKSLDPTAVAKAME
                     KMEWDGLTGKESMQAFDHQTNKAYYLLVAKQKKDIKGPGDLATVISSGRSLIPESKTE
                     CRMK"
     gene            7083..7466
                     /locus_tag="VEL68_07350"
     CDS             7083..7466
                     /locus_tag="VEL68_07350"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXZ35816.1"
                     /translation="MNPLFTFLSAAYIFFIFYLADSSVVSKIGEFNFYSLLHIPLYGI
                     LMTLLLLAFASKPETSRKSRYSGAAWLAIAVGALDEYYQSFIPSREGSLGDVLLDILG
                     IVLVMILARRVPPLLWMNALKKLKK"
     gene            7515..8303
                     /locus_tag="VEL68_07355"
     CDS             7515..8303
                     /locus_tag="VEL68_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35817.1"
                     /translation="MQKKMKAVVPIAFLVFLWPALSWAQFDLFNAEDYNIEVEGRYWY
                     PKLASSLKVEESGIGTEFDAVNDLGFDEKKGFGEGRLQIKIFNRHKFNFSYLPMKWEG
                     DKVLTRTIQFSGQTYTAGTRVQSEADLKLFKAGYELDFIVSQYGFLGGSLDVMVTDVH
                     AQLKAPSLALDEKEDFTVPIPMVGLIGRITPIKWINLTAKASGLPLGSYGHAFDAEAY
                     LEINPIKYVGISAGYRYFSFLGQYDKNKADFRLDGPFTALKIRF"
     gene            8587..9495
                     /locus_tag="VEL68_07360"
     CDS             8587..9495
                     /locus_tag="VEL68_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35818.1"
                     /translation="MGELRVSDIKKVTVIGAGLMGAQIGELIARIGKCRVTLVDIKDE
                     FVNKGLAGIDQRTEKFFVAKGKMTPEEKKELMSRISGTTDMAKAAGASDFILEAATEN
                     IKIKKELFNKMGESAPAPAILATNTSYLSITEIASATNRPDKVVGMHFFNPVGVMKLV
                     EVVKGAKSSENTVAVTSDLARLLQKEPVVCWDASYGFLANRAYFAMRMEAVQMVYERV
                     APPEEIDKALKLGYNLPMGPLELADMGGSWGIYASSEEDRIRELGSERGKLHPLVRLM
                     VRAGYTGGAGKKGIYDFYKEVLSKMK"
     gene            complement(9590..10756)
                     /locus_tag="VEL68_07365"
     CDS             complement(9590..10756)
                     /locus_tag="VEL68_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ35819.1"
                     /translation="MPKKATTKTLNRRERFLKTLNHQEPDRVPIDLGSVGGGITDVAY
                     SRVKEYLGIKGDEGTTYTTTLTISQFDERVLQALNVDVRRVGLRSSKRRPGRVEQLDG
                     SWADEWGIFYRKAGYYNQIVKNPLRNATVADLARHPWPDPGDPNLVEGLAAQARHLYE
                     GTDFALSAKSVSGGIFQTCCRLRSMEQFLMDMLTDKPFARELLARVEESVLSLTDALL
                     TATGLYVQMVETQDDLGTQRAPLISPALYREMIQPCHANLSALIKKKTEGKARVFLHS
                     DGSIFDVLPDVIDAGIEVLNPLQPHAAKMDPKGLKAAFGDKLVFHGGLDQQQIIPFGS
                     PEEAAADVRRVIRALAPKGGYIFAPCHNLQPDVPPENILAIYRAAAEYGVYPIS"
     gene            complement(10872..12158)
                     /locus_tag="VEL68_07370"
     CDS             complement(10872..12158)
                     /locus_tag="VEL68_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35820.1"
                     /translation="MDALSGILMILCFFVVVAVIMKGESPIIMLLLLTIAWALLAGIP
                     GKNILATVLEKGGTAYASTIVIIIFGAWFGQTLVKTGIAESIIRGAIELAGDRPIVVT
                     MVVTVVTALLFISLYGVGAAIALGVIALPIIMSMGVPPQVAAASYVMAIGAGTFLNLV
                     DFNIRAPLFPGIKYEGPLLTFFAAAWVVYTLAALAMIFFNLKIRGGVRKYSAVSIAPP
                     VTSRPRVSWYAYISPAVPVVMVIFFKWPMIPAFILGVAYALLTTHFGKRTWRETIDLF
                     HRSFYDAFPEIATIAALWIICGMLIVGGQLPEIQKVLKPIYSPFLPESRVMLTLFFIV
                     LTPLAIYRGPLHVVGTGAALIGMFLNAKTFPVLQLFAAWRGAMAVGSSMDPTNSWTLW
                     TIGYTKITHKQYLATGFPWAWLMVAIVTLLAYYLIP"
     gene            12476..13537
                     /locus_tag="VEL68_07375"
     CDS             12476..13537
                     /locus_tag="VEL68_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HXZ35821.1"
                     /translation="MKASAKVDGLRKFVEQVYQTVGASKEYAQISAEVMVEANLCGVD
                     SHGVRMLPGFVTLSRNGKINPKGKIKVIRESQVIAHLDGGLAPGHVVSVQAMKMAVEK
                     AKASGIGFVLVRNSTHWGRAAYYPVLAAQEGCVGVCFVNTESNMPYWGTRAPRIGNNP
                     LSIGVPRASGEPVVLDMAMSQAAFFKIVLYNREGKKVPLGWGVDEQGRPTDDPAAILK
                     SRRLVPMGEHKGSGLALMIDILTGVLSGGMFCGELLGEAKGMPHATAYAQAFMAIDIA
                     TFLPLEVFKQRVDKLVTYIKEGPLAEGFSEISIPGERSWRERALRERVGIPLDEITLG
                     ELRALAGQLGIPCFPVSQE"
     gene            complement(13704..13889)
                     /locus_tag="VEL68_07380"
     CDS             complement(13704..13889)
                     /locus_tag="VEL68_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35822.1"
                     /translation="MADFEMPHSGHERHLCFLHNVGMVKEKIEEYKKLVKEGKYVCKG
                     CGRVAVNEKHLCAPEKL"
     gene            14084..14707
                     /locus_tag="VEL68_07385"
     CDS             14084..14707
                     /locus_tag="VEL68_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXZ35823.1"
                     /translation="MRIMVIVMLVAVFVVGISTFANAQAKEPRFPELKREQMTEAQKQ
                     VYDAIAGGPRGSVRGPFEPLLRSPVLTDRVQKLGEYLRYGTSLPARLNEFAILINARF
                     WNSKYEWYAHKPLATKGGLSESIANDLAQSKRPMNMKPDEELVYEFCTTLHREHFVSD
                     ALFKRAMATLGEQGVIDLIGVSGYYTLVSMVLNVAEIALPPGEKSPW"
     gene            14760..15155
                     /locus_tag="VEL68_07390"
     CDS             14760..15155
                     /locus_tag="VEL68_07390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXZ35824.1"
                     /translation="MKLIYLQPDNVSRPAGDYSQGLKVKGGTLVVISGQVAWDVQGNL
                     VGKGDFRAQTRQVFENLKNMLASAGATFKDVVKLGIFLKNRDDFAAFREIRAQYLTPP
                     FPPTTLLVVKDLAREEWLLEVEAMAVVEK"
     gene            15289..>15711
                     /locus_tag="VEL68_07395"
     CDS             15289..>15711
                     /locus_tag="VEL68_07395"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXZ35825.1"
                     /translation="MKRQKDSSGVRILLVRHGETDWNLAGRFQGRSDVPLNQKGVEQA
                     NALASALRKESLTAIYSSPLGRAKETARLIKVFHPSAPLYEEEGLMEMDLGEFEGIEG
                     ARWAEKHASFRQAWRDNPAAVTMPGGESLKEVQARAVAA"
BASE COUNT         3715 a         4216 c         4300 g         3480 t
ORIGIN      
        1 ttctttggtc ccttctccat cggtgaacct aatcacccct aggatggtga gcattttcaa
       61 agctcctaaa cataaattca gagagaattc aaatctcatt cgtcaaagga tgtatcgaac
      121 gcggtatttg ggtccagtgg aaagattggg cggcgcaatt tggcgaacgg gaactgacgg
      181 tagtcagaac ctgtcactcc gactccgttg cattcgatta ccgctttgga aatgggcagg
      241 aagccagcgc ggtaatgaat cctcgattta agcagcagaa attttttggc ggtcggctcg
      301 atgccgacgc tgcggaaaat ccccaagtca aaaggttcgt ggttttcttc tatgactaca
      361 acctgcatgg cccccgtatc caggaccacg gcccggccca gggtcatcgt aacccccgta
      421 aacatcgggc cccgggcgat aaaccggcca tctgaaatag tccgcaccct tccggtaagt
      481 tcaaaaggtt cacctttgcg tctgatcgcc ggcatatcca tcttgccccc gagcggcaag
      541 gtgaccgtac tcccgactcc ggccttgatc atcgttgcca ccgcttcagg gtcccgaatg
      601 gcgcctacgg ctacatcctc caaaccctgg cggatcactt ctgccacgac ggccatggta
      661 tcctgggttc cgccggaggc acagttgtcg gcgtggtcga ttaacaagat cgggccttct
      721 tttatcgctt tagcccgggc aaccgattgc tccaggggtt cggcccggta gaggaattct
      781 tcccgccgcc cccatgcctc ttttgccaat tttcggcaga tctccccggc ccttcgccga
      841 tcgccgtcgg tgacggcgag gacacttagc ccggcctggt agatatcggc cagaggaaag
      901 cccccgaaca cgctaacggc tagagcgttc ttttcctcga gctgcgccaa agaaatgaga
      961 tttcccattg ggggctcatt cgtgcccatg cgcaaagtat gggggatcat cgggcaactc
     1021 atccaggcca tggtcggatg gatgcgccct tttaaagatt cgatgacgat ctggcctact
     1081 tttttcccgg tttcatacgt atccacgtgg ggataagtct ggtaaccggc catcgcggtg
     1141 cagtgagcta ccatctcccc cgtcaaattc gtatggaggt ccaaggctac ggcgatgggc
     1201 aactccgggg ctatactccg gattcgcttc aaaagggtgc cttctccatc atcggtccct
     1261 tgggacacca tggccccgtg gagatcaaga aatagggcgt cgcaaccccg cgctacggat
     1321 tcacaaatcg ccctactcat ctcctcgtag gccgcggatt cgactggacc gctgggccag
     1381 gccgaggcgg caatcggagt gacgatttcc gccccttctt ttcgggccag atcgatgaaa
     1441 gccgccatgg gggtattggt cccctgatag gcacccaggg ccttcgaccc gaaaatatct
     1501 ccctgccggc caaaactggc taggggagtg gggaccggag agaaagtatt ggtctcatgt
     1561 ttcatcatgg caatgacaaa acgcataatt cacccccagg accttgtgga ttttttaatc
     1621 tccctccatt gcctgctcga ccgcgctgag ggttagatat cgatctttaa cctcctcatt
     1681 ggccatgaat tcctcattgc tcccgtggta gacaatggaa ccttgatcga taatgtaatg
     1741 atggctacag agcgcggtgc acatggccaa gttttgctca accaggagga ccgtaatatt
     1801 ttcccggaga atttcctggg tgatcctctg cagctcctgg acgatgatcg gagcgagtcc
     1861 ctccgaaggc tcatccaaga gaagcagctg gggctcgttc atcaaggccc gggcaatgga
     1921 gagcatctgc tgctccccgc cggagagttt ggccccccga tgttgccggc gttcggccaa
     1981 tttcggaaat tctgcaaaca cccgatcgag ggtccaagtg ccctgatgat ctttcccctt
     2041 cgccacgcgg gcaatttcca gattttcgac taccgttagg taggaaaaaa tcgctctttc
     2101 ttccgggaca taagagatac ctttccgggc aatccggaat ggcttcagtc cgcaaatctc
     2161 ctccccctta taccgaatac ttccttcttt ggggtgaacc agtcccataa tagtcttcaa
     2221 agtcgtggtc tttccggccc cattccggcc caggagcgaa acggcttggc cctgctcaat
     2281 ggaaatattg attccctgga ggacgtggct ggtcccgtag taggagtgta tgtttttgag
     2341 ggcgatcatt ccgcagctcc tcccaaatag gctttccgga ctttctcgtt cttctcaatc
     2401 tgagagggag ttcccgtggc gatcacttgc ccctgatgaa ggacggtaat caccgtggag
     2461 atcgaccaaa ccagctgcat gttatgttca atcaccacca tcgcccgttg cttggccaat
     2521 cttttaatca gccgaatcat ccggatggtc tcttcggggg acatcccgct ggtgggttca
     2581 tccagcaata acagcagggg attggcggcc agagcgatgg ctacttccag ggtgcgctgg
     2641 cccgcatggg aaagctctcc agcttttttc gccacaatgg gggtcatctc caattcttcg
     2701 agcactttcc gggcctgttc gacgggggag ggataagaat gaaaatcttt caggaaatta
     2761 aagtgccccg gatgctgact atgggctgcc agccgcaaat tttccaggac ggtcagattc
     2821 ttgaagaggc tatttagctg aaaggaccgt ccgatgcgca tcttggccac ctggtgaggt
     2881 ttcagcccgg taatattttt ctcttgaaaa aatacttccc cctccgtggg agaaatttcc
     2941 ccggtcagga tattaaaaaa ggttgtcttt cccgcgccgt tgggtccgat gatcgaatgg
     3001 acggccccct cctctacctc catatttaca tggtctacgg ccatcaggcc gccgaagttc
     3061 ttggtcaggt tcacagtccg caggatcatg actttccccc ctgtcctgcg cgcgtagcgg
     3121 tgaatttatt ccagctgtcg cttaggatcc cccagatccc cccccgcatg aaaaggacaa
     3181 aacccacaaa aatcaccccc aggatgaaaa gccacctcgc ccagagcacg ctcaaaattt
     3241 ctgaagcgac tttgacaacg atggccccga taatcggccc gaataaagac cccgtgcccc
     3301 ccactaagga catgatgatg aaatccgtcg aggtgcccag ctcgatggcg ttgatgggta
     3361 ccatctttat gaataaagca tagagtcccc cagccacccc ggtaaaaaaa ccggagaggg
     3421 tgaaagccat caccttataa tgtatgacat tatatcccag agctcgagtc cggtcggggt
     3481 tttctcgaat ggccatgagg acccgaccga aaggagaatg gatgatccgt cggattacga
     3541 tgaaagacag gagaaatatc atccagacaa aaaggaagaa ctgaaaattg ccctggatgg
     3601 gaagttctaa tcccgggaag agggaaaagt ccggccgggg tacgttcaaa aggccatcat
     3661 ccccgccggt gatccctttc cattggtacg ccatgaaaaa agccaattgg ttaaaggcca
     3721 aggtgagcat tacgaaataa atccccacct gctgaatgga aaggaaccca attactgcag
     3781 caaatagggc cgccaggatt cccgagagag ccaaagtcag tataggatgc aaaccgaagt
     3841 gaatcagcaa cagacccatc aggtaagccc cggagccaaa aaaaatggcc tgaccgaaag
     3901 acagaaggcc agtatagccc agaagcatat tgaaagagac ggcccccaag gcatagatga
     3961 cgatctcggc tggcagggtt ttatagggca gcaggagagg cagcaagagc agcaggcccg
     4021 ccaagatgaa aatctctcca tcgatccaac tttttctttt tccgagtgct ttttcgatca
     4081 tattttcttg tccaccaggg agcagcttcc cgcaaatgcc ggccaattat agacctcttc
     4141 tcttggggat ccccggggcc catttcgacg tgtccgccgg gagaaattgc gggctgatgc
     4201 caaatcctga aaattggcgt tcctctgcgc cgcaacgcag aattatctga agcccaagag
     4261 tccccttggt cggatcagga tgaccagggc cattgccgca aaggtggcga tcatggcgcc
     4321 gggcggccag accagggcca tcatcgattc gacgatcccc acgatgagac ctccgaggat
     4381 cgcgccagtg aaacttccca tccctccgat gaccacgacc acgaaacaga gggtcaacac
     4441 tgaatccccc atgaggggca tggccccccg catgggcaaa ctgacggctc cggccaatgc
     4501 ggccaatccc atccctatgg aaaaaacgac cgtgaagacc gtgtatatat taatccccag
     4561 gctgttcacc atattgcggt tttcggtgcc cgcccggatg atggaaccgt aactcgtctt
     4621 ctcgatgagt aaccacgaaa ttaaacagat ggccgcgata aagacgacaa gaaagatgcg
     4681 gtattcgggg aaagaaaaag tccccaaatc caccactccg gccagttgct tcggcatggg
     4741 cacactcttt ccgacgggcc cccagatcat gatgatgacc tcctgaagga tcaaggttat
     4801 cccgaaagtc aggaggatct gatagacatg ttcctcatca tacattttcc tcagcagcac
     4861 gacttcaagc agtgccccca gcagcccggc tatgaccgga gagagaaaaa tcgccaccca
     4921 gaagctgccg ctccattccc ccagcaggac tgaagtttga aaagctaggt aggccccgag
     4981 ggcatagacg gcgccatggg caaagttgat gatgttaagc atgccgaaga tgaccgttaa
     5041 gccaaccgcc atcaggacat aaatcatgcc caaagaaatt ccgttgaaca attgaactaa
     5101 aattagttcc atgaaaaagg agttccttct ttagagaggc ccgacaaaag gtctgcagga
     5161 ctccggcccc ccggaagccg tttcccctat tcacccggtg aatgggaaat cttttccgct
     5221 ccgcgaggcg ccgcggaaaa agttaccgtc attcccccgg aaggcggaat gacggttttc
     5281 tacacaagca tcagattgtc tcgccaatac ttcaaaggtg cgatggccgt cgggtcaagg
     5341 cccatggact ctctgcggcc ccgaacccta tttcatccgg cactccgttt tggattccgg
     5401 tatgagagac cggccggagg aaataactgt cgccaggtct cccggtcctt ttatgtcttt
     5461 cttctgcttg gccaccagta gatagtatgc tttgttggtc tgatggtcga aggcctgcat
     5521 ggattctttt cctgtaagcc cgtcccattc cattttttcc atggctttgg ccacggcggt
     5581 cgggtccagg ctcttggctt tttcgattgc caggataatc atccgggtca tgatgtagga
     5641 agaggctgcc ggataggaga taatatcctg gaatttggcc tggtatgctt tggtcaacga
     5701 ttgagtcgcg ggcacaggca catcatgcca gaactggcag ccaaaataga ttccctccgc
     5761 ggtttcgggg ccgatctcct gaaggtccat caggccggag gaccaggcca ccaggagttt
     5821 tgcctttttc ttcaatccaa aattgtcggc catcttgaga gcattaatgg tatccttccc
     5881 gaaattcaaa aagacgacaa agtccgactt tgccgccatg gctttcgtaa agaaggagct
     5941 gaactccgtg tgaccgatgg ggtgcatgtc attgcccaca tgctgaattc catattgctt
     6001 gaaaacctct accgcgttat tcagcagaga atgaccgaat acataatccg gggtaatggt
     6061 ataccatcgc ttcgcttcgg ggtacttttt aatgagcggg ataatggtct gctggatggc
     6121 cccataggta ggaagggacc agcggaaggt atagaaattg caggccttgc cggtgatctc
     6181 gtccgctccc acggagcaga catagaccgc cttcttttca ttcgccacct ggcttaccgc
     6241 cagggcaacc gccgaactca ccgccccctg gtagaacttg atccctcggg aatcgatcgc
     6301 ttcccggact ttccgggccc ccactcccgg tttggcctca tcctcgatgg cataggcctc
     6361 ggccggtttc ccaagaatct tattgttgac ctccccgatc gcatacttcg cccctttgag
     6421 gccatggtcc ccaatggccg cataggcgcc gctgaaggtc gaaaccatcc cgatttccac
     6481 tacctccgcc gctccggcgg gcagaatcag ggcgagcata aatgtcattg ccagagtcat
     6541 caatcttatc cttttcattt tcccactcct cctttggtaa gtttttatgc gctcactttg
     6601 cttcgtcgaa tgggttccgc atttacctgc gagaaaaaaa tcctcctcca tgtcaatatt
     6661 ttccctttta tcgttttttc tatgtgatac gctactctgg cagacagact ggcgaaaaaa
     6721 taatagtctt cacattaaaa aatccttata aaaactaatg ttagctacca tattgttaaa
     6781 atcatgtcaa gattttcgta aacagaacct ggagatgtgc ttggtcctgg ccaagcgtca
     6841 ctccctgatt ggatcacggg ctgatcgtgt atcacggaac caatgaagag tttaaggcca
     6901 ataacgaagt gaaagaccga tatctcgctc tgagttccct ggccagggag ttttcggaaa
     6961 aggaggagag ctgatcgaat ttgacagaat ccgggtccca tgatagaaaa aaagagctct
     7021 ttgcgggggt ggagtttcct tactcttgaa gaagtccggg ggagaagcat cagcgacaga
     7081 agatgaaccc tcttttcacc ttcctatctg ccgcctatat ttttttcatc ttttatctgg
     7141 cggattcttc agtcgtctct aaaatcgggg aattcaactt ttacagcctc ctgcatatcc
     7201 ctctttatgg aatactaatg acacttcttc tactagcttt tgcctccaaa ccggagacca
     7261 gccgcaaatc ccgctattcc ggcgctgcct ggctggccat cgctgtgggg gcgttagacg
     7321 aatattacca gtccttcatt cccagccggg agggctcctt gggagatgta ttgttggata
     7381 ttctggggat tgtcttggtt atgattctgg ctcgtcgggt ccctccgctt ctctggatga
     7441 acgcgctcaa gaaactcaag aaataggcgc tcttatccga tatggaagaa taatctcttt
     7501 ttaggggggg gtcaatgcaa aagaaaatga aagcagtggt gccgatcgcc tttctagtgt
     7561 ttctctggcc ggctctctct tgggcccaat ttgacctgtt caatgccgaa gattacaaca
     7621 tcgaggttga agggcgatac tggtacccca agctggcttc tagtttaaag gtggaagaaa
     7681 gcggcatagg gacggaattt gacgccgtga acgatctagg gttcgatgaa aagaaaggtt
     7741 ttggagaagg gcggttgcag atcaagattt tcaaccggca caagttcaat ttttcttacc
     7801 ttcctatgaa atgggaaggg gacaaggttc tgacccgcac gatccaattc tcgggacaga
     7861 cctacacggc ggggacgagg gttcagtccg aggcagacct gaaattgttc aaagctggtt
     7921 acgagcttga tttcatcgtc agccaatatg ggtttttagg gggaagcctg gatgtgatgg
     7981 taaccgatgt tcatgcccag ctgaaagccc cgtccttagc cttggatgaa aaagaggact
     8041 ttaccgtccc gatccccatg gttggcttga tcggcaggat tacacccatc aaatggataa
     8101 atctgacggc caaagcctca ggacttcccc tgggaagtta tggacacgcc tttgacgctg
     8161 aggcttacct tgagatcaat cccatcaaat atgtgggaat ctccgccggg taccgttatt
     8221 tcagcttttt gggccagtat gacaaaaaca aggcggattt caggctcgac ggccccttta
     8281 ccgctctaaa aatccgcttc taagcaacct tttcaagaga atttggtctg gtccgcccct
     8341 ccaaaatcct tccccttcct cccaaaaaga ggaaggaggc cataatagaa tggggccgct
     8401 ttctttctgc ggcccccatt tggtcgtcag tctcatgaga ggaatcctct tccccattca
     8461 acctattttt ccatttgaca ccctagcacg cttcttccta tactgacttc gcaaaaagaa
     8521 tcagcttatt caccctgtgg aagcaaaaaa aactgaataa tgattaatct aaagaaagga
     8581 ggaagcatgg gcgagctgcg ggtgagcgat atcaagaagg tcacggtcat cggggccggg
     8641 ttgatggggg cgcagatcgg cgaactgatc gcccgaatcg ggaaatgccg ggttactctc
     8701 gtggatatca aggacgaatt cgtgaacaaa ggcctggcgg ggattgacca gcggacggaa
     8761 aaattcttcg tggccaaggg gaagatgacc cccgaagaaa agaaagaact catgagccgc
     8821 atcagcggga ccacggacat ggccaaagcc gcaggggcgt cggacttcat cctggaggcg
     8881 gcaaccgaga atatcaagat caagaaagaa cttttcaata aaatgggtga gagtgccccc
     8941 gcgccggcca tccttgcaac caatacttct tatttaagca ttaccgagat cgcctcggcc
     9001 accaatcgtc ccgacaaggt ggtgggaatg catttcttca acccggtggg ggtgatgaag
     9061 ctggtggaag tggtgaaggg ggcaaagtcc tctgagaaca ccgtggcggt aacttccgac
     9121 ctggctcgct tgctgcagaa agagccagtc gtgtgttggg atgccagcta cggattcctg
     9181 gccaatcggg cctacttcgc catgcggatg gaagcggtcc agatggttta tgagagggtg
     9241 gcccccccgg aagagatcga caaagccctg aagctcggct acaacctgcc catgggaccc
     9301 ctggagctgg ccgacatggg ggggtcctgg ggaatctatg ccagctcgga agaagaccgg
     9361 attcgggagt tggggtcgga gaggggcaaa ctgcatcctc tcgttcggct catggtccga
     9421 gcgggctaca ccgggggcgc ggggaaaaaa ggaatctacg acttttacaa ggaagtgctc
     9481 tccaagatga agtagggcgc ttcgcgaagc gctcctactt ggaataacac tccactctgc
     9541 agggcaatca ccccttggcg tgattgcccc cacggatttt tcttgccttt caactgatgg
     9601 gatacacccc atactcagcc gcggcgcggt agatggccag gatgttttcc gggggtacgt
     9661 cgggctgcag gttgtggcag ggggcgaaga tataaccccc cttgggggcc agggcccgga
     9721 tgacccgccg aacgtcggca gccgcctcct cggggctgcc gaagggaatg atctgctgct
     9781 gatccagccc tccgtgaaat accagcttgt cgccgaaggc ggctttcagg cccttcgggt
     9841 ccatcttggc tgcatggggc tgcagcggat tcaggacttc gatccccgca tcgatcacgt
     9901 cgggaagcac gtcgaagatc gacccgtcgc tgtgcaggaa taccctggcc ttcccttccg
     9961 tctttttctt gatcagcgcc gaaaggttgg catggcaggg ctggatcatc tcccggtaaa
    10021 gagccggaga gatcagaggg gcccgctggg tccccaggtc gtcctgggtc tccaccatct
    10081 gaacgtacag ccctgttgcg gtcagcagag catccgtcag gctgagtacg gattcctcca
    10141 cccgggccag caattcccgg gcaaaaggtt tgtcggtgag catatccatc aggaactgtt
    10201 ccatgctccg cagccggcag caggtctgaa aaattccgcc cgagaccgac ttggcggaaa
    10261 gggcaaaatc cgttccttca tagagatgtc gggcttgggc cgccagccct tcgaccaggt
    10321 tcgggtcccc ggggtccggc caggggtggc gcgcgaggtc cgcgacggtg gcgtttcgca
    10381 ggggattctt gacgatctgg ttgtagtagc cggccttgcg gtaaaagatt ccccactcgt
    10441 ccgcccaact cccatccagt tgttcaaccc gcccgggtct cctcttggag ctccgcaggc
    10501 ccacgcggcg cacatccacg ttcagcgcct gtagcacccg ctcgtcgaac tgcgagatgg
    10561 tcagcgtcgt ggtgtaggtc gttccttcat cacccttgat tccaaggtat tctttgaccc
    10621 ggctgtaagc aacgtcggta atccccccgc ccaccgaccc caggtcgatg ggcacccggt
    10681 cgggttcctg gtgattcagg gtcttgagga accgctcccg gcggttaaga gttttggtgg
    10741 tagctttttt gggcatgatt gtctccttga ttccgatgcg gacttaccgt tcgttcatgg
    10801 ttttctttcg gctcccaaag tctaatgaaa tctgacgaac ctcttacccg ggggtccgcc
    10861 ggaaaatatt ctcaggggat taggtaatag gccagcaggg tgactatcgc caccatgagc
    10921 caggcccatg ggaaacccgt ggccaggtac tgcttgtggg tgattttggt gtagccgata
    10981 gtccaaagag tccaggagtt ggtagggtcc atagagcttc ccacggccat ggcaccgcgc
    11041 caggcggcaa acaattgcag gacggggaaa gttttagcat tcaagaacat cccaataagc
    11101 gctgccccgg tgcccaccac gtgcagcggt ccccggtaaa ttgccaacgg cgtcaggacg
    11161 atgaaaaaga gggtcaacat cacgcggctt tcgggcagga agggagaata gatcggcttg
    11221 agcaccttct ggatttccgg cagctgccca ccgacgatga gcatcccaca tatgatccac
    11281 agggcggcga tagtggcgat ctccgggaag gcatcataaa aagaccgatg gaagaggtca
    11341 atggtctcgc gccaggtccg tttaccgaag tgggtggtca gcagagcgta ggcgaccccg
    11401 aggataaaag ctggaatcat gggccatttg aagaagatga ccatgaccac tggaaccgcc
    11461 ggagagatgt aggcatacca ggaaacccgg ggccgggaag tcaccggggg agcgatactc
    11521 accgccgagt atttccgcac ccctcctctg attttgaggt taaaaaagat catggccagc
    11581 gcagccaggg tgtaaaccac ccaggcggcg gcgaagaagg tcaggagagg gccttcgtat
    11641 ttgatacccg gaaagagggg ggcgcggatg ttaaagtcca ccaggttgag gaaggtgccc
    11701 gcgccgatgg ccataacata ggaagcggcc gccacctgag gaggaacccc catggacatt
    11761 ataatgggaa gggcgatgac cccgagggcg atggccgcgc ccacgccata gagggaaatg
    11821 aacaggagcg cggtcaccac ggtaacgacc atggtgacga caatggggcg gtctccggcc
    11881 agctcaatgg ctcctcggat gatgctctcg gcgatcccgg tcttcaccag ggtctggccg
    11941 aaccaggcgc cgaagatgat gatgacgatg gtactggcgt aagcggttcc acccttctcc
    12001 aggaccgtgg ccaggatgtt ttttcctgga atgcccgcca ggagagccca ggcaatggtg
    12061 agcagaagca gcatgatgat gggactctcg cccttcatga tcacggcgac caccacgaag
    12121 aagcacaaga tcatcagaat gccggacaaa gcgtccatct tccactcctc ctattattga
    12181 tttcccgcgg tgctcaaaaa tccatttcct cgacttgcgg ctgaagtcat ggaaaatttc
    12241 cggaaatttt aacacaggga aaaaacattt tcctcctttt tctatttttc tttgacaacc
    12301 aagaggaaga atcgtttcat ttgataagat tcccgatttg gcgtagactc gaaaggattc
    12361 ctggttatcc taaaggctgc aaaaaagaat tcaaaaacat cacccccacc attccttcca
    12421 ccctccgagg tggaaggtta gggtggggcg gaaatatctt gcatcgagga ggaagatgaa
    12481 agcaagcgct aaagtcgacg gtttgcgaaa attcgtggag caggtttatc aaactgtagg
    12541 ggcttcgaaa gaatacgctc aaatctcggc ggaggtgatg gtggaggcca acctctgcgg
    12601 ggtggactcg cacggggtaa gaatgctccc ggggtttgtt accctgtccc gcaatggaaa
    12661 gataaatccg aaaggaaaga tcaaggtaat cagggagagc caagtcatcg ctcacctgga
    12721 tgggggtctg gctccagggc atgtggtctc ggtgcaggcc atgaagatgg cggtggagaa
    12781 agccaaggcg tcagggatcg ggttcgtcct ggtgcgaaac tcaacccact ggggaagagc
    12841 cgcctactac ccggtgctgg ccgcgcaaga gggctgtgtg ggggtctgct tcgtcaacac
    12901 cgaatccaac atgccctact ggggaacccg ggcgccccga atcgggaata atcctctctc
    12961 catcggcgtc ccgagggcct cgggcgagcc ggtggtcctc gacatggcca tgtcccaggc
    13021 cgctttcttt aagatcgtgc tgtacaaccg ggaaggcaaa aaagtacccc tggggtgggg
    13081 agtggatgaa cagggacgcc ccacggatga ccccgcggcg atccttaaga gcaggcggct
    13141 tgttcccatg ggtgagcaca agggatcagg tctggccctg atgatcgata tcctgacggg
    13201 tgtgctgtcc ggggggatgt tttgcgggga actcctgggg gaggccaagg ggatgcccca
    13261 cgcaacggct tacgcacagg ccttcatggc catagacatt gccacctttc ttcccctgga
    13321 agttttcaag caaagggtgg ataaactcgt gacctatatc aaagaggggc cgctggccga
    13381 gggcttcagc gagatcagca taccgggaga aaggtcctgg agggagaggg ctctgcggga
    13441 gagggtcgga attcctctgg acgaaattac tttaggcgaa ctccgggctc tggccggtca
    13501 attgggaatt ccctgtttcc cggtcagcca agaataaaag ggaagactca gtcgcaattc
    13561 gctgttcatc cttcggcacg ctcaggacga acggctgaat aatgtgccgt aatccgttcg
    13621 tagtggacct gtcgtaccac gaacggacct gcgacagagt gtcgtggggg aaggtggggg
    13681 aaatgaggtt atttagaggc catttacagc ttttccggag cgcaaaggtg cttttcgttg
    13741 acggccaccc gcccgcaccc cttgcaaaca tattttcctt ctttcaccaa tttcttatat
    13801 tcctctattt tctctttaac cataccgaca ttgtgcagga agcacaaatg tctttcgtgc
    13861 cctgaatggg gcatctcgaa atcagccatc tccctttcct cctttcctca tcatttcctg
    13921 gggcaagaag atttaaccca ttaatttatt atattcagaa aaattcaaaa agggaaatga
    13981 ggaaaggctc aaattccgtt gacacccagc cgccttccca gtacactcac ggtaccccaa
    14041 tactaactct catcccacaa tcaaaaaaag ggaggaaata tcgatgcgaa tcatggtaat
    14101 cgtaatgctg gtggcggttt ttgtcgtggg aatttcaaca tttgccaatg cccaagccaa
    14161 ggaacctcgt tttccggaac tcaagcggga gcaaatgacc gaggcgcaaa agcaagtcta
    14221 cgatgccatc gccggggggc ccaggggaag cgtgcgaggt cccttcgagc cgctgctgcg
    14281 cagcccggtg ctgaccgatc gggtccagaa gctcggagag tacctccggt atgggacttc
    14341 gctgcccgca cgcctcaacg aattcgcaat cctgatcaac gcccgtttct ggaactccaa
    14401 gtatgagtgg tatgcccata agccgctggc gaccaagggc gggctctccg agtcaatcgc
    14461 caacgatctg gcgcagagca agcggccgat gaacatgaag ccggacgagg agctggtcta
    14521 tgaattctgc accactctgc accgtgagca ttttgtcagc gacgcgttat ttaaacgcgc
    14581 aatggccaca ttgggggaac aaggggttat cgacctaatc ggggtcagcg ggtactacac
    14641 actggtatcc atggtcctga atgtggccga gatcgcgctt cccccgggcg agaaatcgcc
    14701 gtggtgacag agaaggccga aaaaacatgg agcgaaggga tgccaatcag ggggttaaaa
    14761 tgaaattgat ctatcttcaa ccagacaatg tttccaggcc agcgggggat tattcccagg
    14821 gattgaaggt caaaggtgga accctggtgg tgatctccgg ccaggtggcc tgggatgtcc
    14881 aggggaacct ggtgggtaaa ggcgactttc gggcgcaaac ccggcaggtt ttcgaaaatc
    14941 tcaagaatat gctggccagc gccggggcga ctttcaagga tgtggtcaaa ctgggcatat
    15001 tcttgaagaa ccgggatgat tttgcagctt tccgcgaaat ccgggcccaa tacctcactc
    15061 ctccttttcc ccccacgacc cttttggtgg tgaaggattt ggccagggag gaatggctgt
    15121 tggaggtgga agctatggcc gtggtggaaa agtagatttt gctccagctg gttccgtggt
    15181 gaggcgtgcc tatgtcgatt gggcattagg taaaaagtgt tcaccgccac tgtaggggca
    15241 attcaagaat tgcccccgct aggatataag cataggaaaa attgggaatt gaaaaggcaa
    15301 aaagattcgt cgggcgtaag aatactgctg gtccgtcatg gagagaccga ctggaatctt
    15361 gccggcaggt tccagggtcg gagcgatgtt cccctgaatc aaaagggggt ggaacaggcc
    15421 aatgccctgg catcggccct gagaaaggag tctcttaccg cgatttactc cagtccttta
    15481 ggaagggcca aggagacagc ccggctgatc aaggtttttc atccctctgc tcccctgtat
    15541 gaagaggaag gcctgatgga aatggacctg ggggagtttg aaggaatcga aggggcgcga
    15601 tgggccgaga aacacgcgag ttttcgccag gcatggcgtg acaatccggc ggcggtcacc
    15661 atgccgggtg gggaaagcct gaaggaggtt caggccagag ccgttgccgc c
//
LOCUS       DASPRR010000190        12181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_533922, whole genome shotgun sequence.
ACCESSION   DASPRR010000190 DASPRR010000000
VERSION     DASPRR010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12181)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533922"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VEL68_07400"
     CDS             complement(<1..578)
                     /locus_tag="VEL68_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HXZ35826.1"
                     /translation="MRIDRMEVYYVVFPLIYPWRTAYGADPDVHTILVQLQSGNKIGW
                     GETTPLYAPCYSPESSVGVYHTIREYFAPQIVGEDFPTARHIQERLKHFKGNYFSKAG
                     IENAWWLLKAHMEEKPLHQLLGGTRKVVESGTAHGIKENLDMLMEAIQKGIDDGYKRT
                     KLKFAKGWGLEMLKPVRANFPRHTFHIDCNSS"
     gene            complement(717..1835)
                     /gene="menC"
                     /locus_tag="VEL68_07405"
     CDS             complement(717..1835)
                     /gene="menC"
                     /locus_tag="VEL68_07405"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HXZ35827.1"
                     /translation="MRIDRMEVYYVALPLIYPWKTAYGEDADIHSVLVRMFSGDHEGW
                     GETTPFFAPTYSPETSSSAFFLITEIFGPQIVGKDWESASALRDHLAVFKGNPFAKAG
                     VEIAWWNLEAQIRKIPLHRLLGGKTRMVEAGQDFGVQDSFDMLLGNIEKAVKQGFKRI
                     KLKAKPGWDVEMLEAVRGAFPKMTFHIDCNSGYTLGHLPLFKKIDKFHLAMIEQPLFH
                     DDLVEHAELQKQIGTPICLDESIKSPRDMRLAIKLKSCGYVNIKPGRVGGLQNAIEIH
                     NLAQDNGIPAWVGGMLESGIGSGVNIELATLENFTYPGDVFPSERFYVEDLTEPAVRF
                     APGKLGFQPSGKPGIPYAPIMDRIEKRTVRKAVVKTGT"
     gene            complement(1998..3308)
                     /locus_tag="VEL68_07410"
     CDS             complement(1998..3308)
                     /locus_tag="VEL68_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ35828.1"
                     /translation="MDKKKLKELALQAIEREKDKIIGWSKAIAGKPELGYKETQTAEF
                     IRSALEGLGLPTRGGIAATGVEGVLEGKGPAPGLLFMGEMDAVVNRESSAADPVTGAA
                     HLCGHHLQVGIMLGAALGLIQAAARNHLSGKVFFLGTPAEEFIEIEERLRMREQGKIH
                     YLGGKQELVRLGYLKDKHLCLLVHAHSGLSQRKMLFGGRTNGFLAKSIRFHGKPAHAG
                     VSPHEGINALNAACLAIINIHAQRETFREDDKIRVHPIITKGGDVVNVVPYDVRMETY
                     VRGARLEAITDANEKVDRAIHAGADAVGAKVAIQNIPGYLPLIQNPAMSEIAKSNAFS
                     LAGEEGVGAAGFEGGSTDMGDLSYLIPTIQPYSGGIRGNVHAADFEVVDYEAAVIYPA
                     QVMAMTVIDLLFGGAEEAQRVIREFKPEMTADEYLAFLQKVENP"
     gene            complement(3603..4355)
                     /locus_tag="VEL68_07415"
     CDS             complement(3603..4355)
                     /locus_tag="VEL68_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35829.1"
                     /translation="MHGAVCIWQRDMRVLRRNISSEAATVLAFPLTFFLTFGLGLKEY
                     IQDIDGISYALFVVPGLISMTAILGAFDDGSWGMWFHRVVQKTIEEYRVNPISVTDII
                     VGKIISGFTSGFLKGLSVGICLLFLTGFQFNPLYLFSYILFIFIGCIMFSCLGSLAGT
                     LIDKPENLGRLEAVVVMPFIFLAGLFFPLSAYPDTVLPYIKSLPTTALFDGARLALLS
                     GRIEPVYLAELLVSAMISFALAVRLFEKKMHG"
     gene            complement(4355..5281)
                     /locus_tag="VEL68_07420"
     CDS             complement(4355..5281)
                     /locus_tag="VEL68_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ35830.1"
                     /translation="MKSEIEINHVTKAYGKLVAVKDFSLAVPRGTVFGLLGPNGAGKT
                     TLVSILTTLLKPTLGAASVGGYDVVRDGARVRSLIGVVPQENNLDRYLTARENLVLHA
                     KMQRMNPSEANRRIDELLSLLNLADRQTDFPPKLSGGMQRRLVVARALVHDPQILFLD
                     EPTTGLDPQAKRLIWDYFLSLKGKRTLFLTTHNMEEADFLCDQITIIDNGSPIASGTS
                     DQLKEMAESSWFYEIEVHSRAEEYLHLFQRLPFIDCASLQDGEIEVCLKKDGSLGALV
                     ERINPRDLKRISSRQPSLEDVFLKLTGRRLRQ"
     gene            5509..6801
                     /locus_tag="VEL68_07425"
     CDS             5509..6801
                     /locus_tag="VEL68_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HXZ35831.1"
                     /translation="MADFKQMYKTVMEDHFPERMIIQFGNQSLIYRKRAWKIPDEGTG
                     ELIEKGLRYGENPGQEAALYELVNGNLVLGGCEFIQAGKGLVSSISEEDMIQSGKHPG
                     KINFTDVDNALNILKYMMDRPGAAIMKHNNPSGVALSATLADAYGKANMADRIAAFGG
                     ALVVNRPLDKATAEMVSENYLEVVAAPEYEEGTVEVLKARKNLRIVRVPRMDRLAEYR
                     NMRFVDFKSLIDGGIVVQQSSLNAINTKRDFKKATATYKGKPYVVEREPTEEEYADMI
                     FGWAVEQGVSSNSVLYVKKGVTVGIGTGEQDRVGVAEIAAYKAYTKYADALCFRKYGI
                     PYKTLEMEIENGRRDLAAKKEIDHQTQEARGGIKGSVMISDAFFPFRDGVDVGIKEGV
                     TAIVHPGGSDRDFESIQACNEARPKVTMAFTGQRAFKH"
     gene            complement(6927..8177)
                     /locus_tag="VEL68_07430"
     CDS             complement(6927..8177)
                     /locus_tag="VEL68_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35832.1"
                     /translation="MSRRNAMLAVMISLFFFSFFYRTSPAVISPHLLRDFSLGAEKLG
                     LLSSIFFIVFAVAQIPLGPALDFIGPRKVVSVLGMLGALGSLTFALAPSYEVCLLGRG
                     LTGLGMSCMYMGTLTIVANWYPPRQFATLTGIVSSLGNAGALTATIPLALLAGSLGWR
                     GSFHLFAALNLLLAVLAWVVIRDRPARVANQPVLSPRHFSLGKAFRAVLGTPTFWFIA
                     TLNFFTAGSFLAFQGLWGGPFLMDGFELSPVEAGSVLSMIAFGYIIGCPLIGMISDRL
                     VPSRKTLTLIILSIYLVPLLSLSFFLGPGRNFFLFPVFFSIGLFASGVVLSISHLKEL
                     FPTRIVGTALSLNNLFAIGGAGILQYLMGWIIERHPAVGRIYPIEAYQEAFLLLLGGM
                     AGSLLLYLRAKDSNPPSLLSPPKK"
     gene            8327..9508
                     /locus_tag="VEL68_07435"
     CDS             8327..9508
                     /locus_tag="VEL68_07435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35833.1"
                     /translation="MAEPGEAPQKIDYRVLTLLSMGHLVTDITSGALPAMLPFLKEAL
                     GLSYAMAGTIVLVSNLMSSVIQPAFGYLTDRKSLLWFLPLGSFCAALFLALVGWAPSY
                     SLVLVMVVFSGLGVAIYHPEGWRVANFFAGEKKATGMSIFAVGGNLGFALGPLMATFF
                     ITHLTLKGTTLFVIPGAAIASVFLFSRFWRVDTTAIKKKTASDPALPALRKALYPMTL
                     LLGMIMFRSWAHIGMLTFIPFFYINYLKGDPMLAGNLLFAFLAAGTAGTFIGGPLADR
                     FGHKRIILFSLGLSCPLFILFLLSSGIWSFIWLTLAGLILIFSFSVSMVMGQSFMPQN
                     VGLASGLILGLSFGMGGLGAAVLGLFADIWGVPTTLWMIAFLPLGAFILAALIPYPLK
                     K"
     gene            complement(9610..10041)
                     /locus_tag="VEL68_07440"
     CDS             complement(9610..10041)
                     /locus_tag="VEL68_07440"
                     /inference="COORDINATES: protein motif:HMM:NF018624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35834.1"
                     /translation="MRIDKEGRWFFQGEEITHRKTYLLYCRSLTRDDAGRIVLRVGRE
                     ECPVEVEDAPFVVMTLEFVSRGPAGEEEVWITLNDETREKLSPETLRIGPGNIPYCKV
                     RNGLFEARFSRNAYQVLLPHILPDERENQFFLSLNGKKYPL"
     gene            complement(10052..11431)
                     /gene="mnmE"
                     /locus_tag="VEL68_07445"
     CDS             complement(10052..11431)
                     /gene="mnmE"
                     /locus_tag="VEL68_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXZ35835.1"
                     /translation="MNPGDTIAAISTPLGVGGIGIVRLSGPQARQVFEKIFRRRGADG
                     PLLSHHFYLGEVLHPQGRAVIDEVLAVFMEKPRTYTREDVVEIQAHSGILILQEILNA
                     VLQSGARLAEPGEFTKRAFLNGRIDLTQAEAVIDLIRSKTRKSLEMANLQRQGRLGTE
                     VRQVRDRLLDLLAQMEASIDFPEDEVPELSGENIHLQLQGALERLNSLLQTYEQGKVY
                     REGVSAVIVGRPNVGKSSLLNALLREDRAIVTPFPGTTRDVIEEGVNVRGIFLKLMDT
                     AGLHQTKDAVEGEGVRRTWDRLAQADLVIWVVDGSEPLTPDDLDILREVRDKRTVVAI
                     NKNDLPLRVNLQDLSGQMPGVPLIPVSALHHRRISHLEDAIRDRVLKGTSESSSEILL
                     SNLRHKQALERAREGLERATEASRLKLSVEFLSVDLGAALQALGEVVGETTSEDLLER
                     IFSRFCIGK"
     gene            complement(11428..12108)
                     /gene="jag"
                     /locus_tag="VEL68_07450"
     CDS             complement(11428..12108)
                     /gene="jag"
                     /locus_tag="VEL68_07450"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HXZ35836.1"
                     /translation="MKSVETEGKSTEEAIHKACEELGAPRQELEIEVLSNGSSGFLGM
                     GAKNARIRATVKEKSAPRISEARPLGAVSDPQAAETAKKILQDILRLLEVEATVDLKE
                     ESERILLNIKGDGSGLLIGRKGQTLDALEYLINKIVHKGAEDKKRIVVDTENYRLRRE
                     DSLVQLAQRLAEKAKRLGRPVTISPMSAHDRRIIHLALQDDKTLRTWSTGSGLYRKVI
                     ISPEKKSS"
BASE COUNT         2723 a         3393 c         3464 g         2601 t
ORIGIN      
        1 aagctggaat tgcagtcgat atggaaggtg tgccggggaa aattggcccg caccggtttc
       61 agcatctcca acccccatcc tttggcgaat ttcaacttgg tccgcttgta cccgtcatcg
      121 atccctttct gaatcgcctc catgagcatg tctaaatttt ctttgatccc gtgagcggta
      181 ccggattcca ccacctttcg ggtcccaccc aggagctggt gaagaggctt ttcttccata
      241 tgggccttga gaagccacca ggcgttctcg attccagcct tggaaaagta gttccccttg
      301 aaatgcttca gacgttcctg gatgtgccgg gccgtcggaa aatcttcccc cacgatttga
      361 ggggcaaagt actcccgaat cgtatggtac acccccaccg aggattccgg agaatagcag
      421 ggggcataga gaggggtcgt ttctccccag ccgatcttgt tgccggattg gagctgaacc
      481 agaatggtat gaacatcagg atcggcccca taggccgttc gccagggata aattaacgga
      541 aagacgacgt aatagacttc catccgatcg atgcgcatgc ttctctcctt tccttaccac
      601 agaggacggg cagagcgcat agaatgaaaa agggatttta gattctggat accggatcct
      661 ggatcccgga catgaaccgg cggcctgctc gagcatcgag gatcaagtat tcagtatcaa
      721 gttcccgttt tcacaacagc ctttcggacc gttcttttct caatccggtc cattatcggg
      781 gcatagggaa tgcccggttt gccagatggc tgaaacccca gcttgccggg ggcaaaccgc
      841 acggcaggct cggtcaggtc ctctacataa aaacgctccg acgggaaaac gtcgcccgga
      901 taggtaaagt tctccaaagt cgccagctcg atgttcaccc ccgatccgat cccgctctcc
      961 agcattcctc caacccaggc gggaatcccg ttatcctgag cgaggttgtg gatctcgatg
     1021 gcgttctgca ggccgcccac ccggccgggc ttgatattta catacccgca ggatttcaat
     1081 ttgatggcca gccgcatgtc ccgggggctc ttgatggatt catccagaca gataggggtc
     1141 ccgatctgct tttgcagttc agcatgctca accaagtcgt catgaaaaag aggctgctcg
     1201 atcatggcta gatggaattt gtcgatcttt ttgaagaggg gaaggtggcc aagggtgtac
     1261 cccgaattgc agtcaatgtg gaaagtcatt ttaggaaaag caccccgaac cgcctcgagc
     1321 atctccacat cccagccagg cttggccttg agcttgattc ttttgaaccc ctgtttcacc
     1381 gccttttcga tgtttcccag gagcatgtcg aaggagtcct ggactccaaa atcctgtccg
     1441 gcttccacca tgcgggtctt tcctcccagg aggcggtgga gggggatttt tcttatttgg
     1501 gcctccaggt tccaccaggc aatctcgact ccggctttgg caaaggggtt ccccttgaaa
     1561 actgccaaat ggtcccgaag agccgaagcg ctttcccaat ccttccctac gatctgcggc
     1621 ccgaatattt cggtgatcag gaagaaagcg gaagaggatg tctccggcga gtaggtgggg
     1681 gcgaagaaag gagtcgtctc tccccaaccc tcatggtctc cggagaacat gcggacgagg
     1741 acggagtgga tatcagcgtc ctctccgtaa gccgtcttcc agggatagat caaaggaagg
     1801 gcgacgtaat aaacttccat ccggtcgatt cgcatctatt ttcctttctt tcccgcagag
     1861 agcgctggaa atgcaggtcc ttcggtccct gcggttggtg gacaacctgt agaggcggcc
     1921 ccctgtggcc gcccgaagga cgggcaacca cagggggttg cccccacaat gccctgccta
     1981 gttctggcca agtcgggtta aggattttcc accttttgca gaaaggctag atattcgtcc
     2041 gcagtcatct ccggtttgaa ttcccggatc acccgctggg cttcttcggc tcctccgaag
     2101 aggagatcga tcacggtcat ggccatgact tgagctgggt agatcaccgc agcttcatag
     2161 tcaaccacct caaaatccgc ggcatggacg tttccccgga ttccgccgga ataaggctgg
     2221 atcgtgggaa ttaaatagga gaggtcgccc atatccgtgg agcccccctc gaagccggcg
     2281 gctcccaccc cctcttcgcc ggcgagcgag aaggcattgg atttggcgat ctccgacatg
     2341 gccgggttct ggatcagggg aagataaccg ggaatgttct gtatagccac tttggccccc
     2401 acggcatccg ctcccgcatg gatcgcacgg tctacttttt cattggcgtc tgtaatagcc
     2461 tccagccggg caccgcgaac gtaggtttcc atgcgcacgt catatgggac cacattaacc
     2521 acatctccgc ccttggtgat gatggggtga accctgattt tgtcgtcctc ccggaaggtt
     2581 tctcgctggg catgaatatt gatgatcgcc aggcacgccg cattcagggc gttgattcct
     2641 tcatggggag aaacccccgc atgggcgggt ttcccgtgga aacggatgga cttggccaga
     2701 aagccgttgg tccttccacc gaaaagcatc ttcctttggg aaagccccga gtgggcatgg
     2761 accagcaggc acaaatgttt gtccttcaaa tatccaagcc ggaccagctc ctgctttccg
     2821 cccaggtaat gaatcttccc ctgctcccgc atgcgcaggc gctcttcgat ctcgatgaat
     2881 tcttctgccg gagtaccgag gaaaaagacc ttgccgctga gatggttacg ggccgcagcc
     2941 tggatgagcc ccagcgctgc cccgagcatg attcctacct ggagatggtg accgcaaagg
     3001 tgagcggcgc cggtcaccgg gtcggcggca gaagactccc ggttcaccac cgcgtccatc
     3061 tcccccatga acagcaagcc gggggcggga cccttccctt ccaaaacgcc ttcaactccg
     3121 gttgccgcga ttccgccgcg ggtgggcaaa cccaggccct cgagagcaga gcggatgaac
     3181 tccgctgtct gggtctcctt gtagcccagc tcgggtttgc cggcgatggc cttggaccag
     3241 ccgatgattt tgtctttctc ccgttcgatg gcttgaaggg ccaactcttt cagtttcttt
     3301 ttatccatgt aattctcccc gcaatctctg ggtgcaagat ctgtccctga gaaaataacc
     3361 ttaaagaaaa atagagtcaa tgccaaagaa gccatgcccc agtggccggg ccggcggccc
     3421 ggaaaggacg gcgaagagat gatcatcccg ctgctccacc agcgctccat tatcaacagc
     3481 ggggaagaag ggaccaggaa acagccagaa gcgagaggtt cgaagaccgg ggctttgcca
     3541 ccaagctcgt gggtctattc ttgtagcctg caaccgctta cccgaaacct catacttggc
     3601 ttttacccat gcatcttctt ctcaaaaagg cggactgcca gggcaaagct gatcatcgct
     3661 gaaaccagaa gctcggccag gtatacgggc tcgatcctgc cgctcaatag agccagtcgt
     3721 gcaccgtcga agagggccgt cgtagggaga ctcttgatgt aagggagtac ggtgtccggg
     3781 taggcggaga ggggaaagaa gaggccggcc aggaagatga agggcatgac caccacggct
     3841 tccaggcgac ccaggttttc aggcttatcg atcagagttc cggccaggga gcccaggcag
     3901 gaaaacatga tgcagccgat aaaaataaag aggatgtagc taaagagata aaggggattg
     3961 aactgaaagc cggtgaggaa gaggagacag attcccacgg agaggccttt cagaaaaccc
     4021 gaagtgaacc cggagatgat cttgccaacg ataatatcgg tcaccgaaat cgggttcacc
     4081 cggtactctt cgatggtctt ctgtaccacc cggtggaacc acattcccca ggagccgtcg
     4141 tcaaaagccc cgaggatggc ggtcatagat atgaggccgg gaaccacaaa caaggcatag
     4201 gagatcccgt cgatgtcctg gatgtattcc ttgagcccca gtccgaaggt gaggaaaaaa
     4261 gtaaggggga aagccaggac cgtagccgcc tcggaggaaa tgttccgcct caggacccgc
     4321 atatccctct gccagataca taccgcaccg tgcatcactg ccgtaatctc cttccggtta
     4381 atttcaaaaa aacatcctcc aaggatggct ggcgggagct gatccttttt aagtcccggg
     4441 ggttgatccg ttccaccaga gcgcccaggc ttccatcctt tttcaagcat acctcaatct
     4501 ccccatcctg gagggaagca caatcgataa agggaagccg ctgaaagaga tggagatatt
     4561 cttcggcccg gctgtgaact tcgatttcat agaaccagga gctttcggcc atctctttca
     4621 gctggtctga ggtgccggag gcgatgggag aaccattgtc gatgatcgtg atttggtcgc
     4681 agagaaagtc cgcctcctcc atgttgtggg tggtaaggaa aagggtccgc ttgcccttca
     4741 aggaaagaaa ataatcccag atcagccgct tggcctgggg atccagcccc gtggtcggct
     4801 catccaggaa caatatctgg ggatcatgaa ctaaggcccg ggcaaccacc agccgcctct
     4861 gcatgccacc cgacagcttc ggaggaaagt ccgtctgccg atccgcgagg ttgagtaagg
     4921 aaaggagttc gtcaatcctc cggttggctt ccgaaggatt catccgttgc atcttggcgt
     4981 gaaggaccag attctcccgg gcggtcaaat agcggtccaa gttgttttcc tgggggacca
     5041 ctccgatcaa gctgcgaacc cgggccccgt cccggaccac atcatagccg ccgacagacg
     5101 cagcccccaa ggtgggtttc aatagtgtag taaggatgct aacgagggtg gttttccctg
     5161 ccccattggg cccgaggagg ccgaagaccg tgccccgggg aacggccaga gaaaaatcct
     5221 tcaccgccac caactttccg tatgctttgg tgacgtggtt gatttcgatt tcacttttca
     5281 ttgaaagatg atctaaaaac aggggcgacc ctccggatcg ctccagtgga tggatttcga
     5341 aatatatttc cagattaaca aataggtcct tccgccgcaa ggagagaaga cagggcataa
     5401 aaagatcttt catttttctt tccccctttg gtaatatcct cgtaggagaa aagggttcaa
     5461 aggattaagg ccccatggaa tcatttaaat cattggcggg aggagtatat ggcggacttc
     5521 aagcagatgt ataaaaccgt gatggaggac cattttcccg aacggatgat catccaattc
     5581 ggaaatcagt ccctgattta ccggaagcgg gcctggaaga tcccggatga aggtacgggg
     5641 gaactgattg agaaaggcct ccgatacggg gagaaccccg gtcaggaagc ggcgttatat
     5701 gagctcgtca acggaaacct ggtcctgggg ggatgcgagt tcattcaggc ggggaagggg
     5761 ctggtcagct ccatctcgga agaagatatg atccagtcgg gaaagcatcc gggaaaaatc
     5821 aatttcaccg acgtggacaa tgccctgaac atcctgaaat acatgatgga ccgtccgggt
     5881 gcggcgatca tgaagcataa caaccccagc ggagtggctt taagcgctac gctggctgat
     5941 gcttacggaa aggccaacat ggccgaccgg attgcggcgt tcggcggagc tttggtggtc
     6001 aatcgtcctc tggataaagc aacagcggag atggtcagcg aaaattatct ggaagtcgtc
     6061 gcggcgccag aatacgaaga aggaacggtg gaggtcctga aggctcgcaa aaacctgcga
     6121 atcgtccggg tcccccgaat ggaccgcctg gcggaatacc ggaatatgcg gttcgtggac
     6181 ttcaaatcgc tgatcgacgg aggcatcgtc gtccagcagt cgtccctcaa cgcgattaac
     6241 acgaaaaggg atttcaagaa ggccacggcc acctacaagg ggaaacccta cgtcgtcgaa
     6301 cgggaaccca ccgaagaaga atacgcggac atgattttcg gctgggcagt ggagcaggga
     6361 gtctcttcca actcggtgtt gtacgtgaaa aagggggtca cggtggggat cggcacgggc
     6421 gagcaggacc gggtgggagt ggcagaaatc gcggcctaca aagcttatac caaatatgcc
     6481 gacgccctct gcttccggaa atatgggatt ccatacaaga ccctggaaat ggaaatcgaa
     6541 aatggaaggc gcgaccttgc agccaaaaag gaaatcgacc accagaccca ggaagcccgg
     6601 gggggaatca agggatcggt catgatctcc gatgccttct tccccttccg cgacggggtg
     6661 gacgtgggta tcaaagaggg agtcaccgcc atcgtgcatc ccggaggttc ggaccgggat
     6721 tttgaatcca ttcaagcatg caacgaagcc aggccgaaag tgacgatggc cttcaccggg
     6781 cagagagcct ttaaacactg attgcggatt tcgcaatgcg gaatcaacgg aatctagatg
     6841 gaagaatgcg gatgctgcgt gctggttgtt aggtaaccga aaaaaatgcg aggaatttca
     6901 ggataggggc gcccagccgg tcgcccctac ttttttggag gagaaaggag tgaaggggga
     6961 ttggagtcct ttgcccgcag gtaaaggaga agagacccgg ccatgccccc cagaagaaga
     7021 agaaaagctt cctgataggc ctcgatcggg taaatccgac ccacggcggg gtggcgttca
     7081 atgatccagc ccatgaggta ctggagaatg ccggcccccc ctatggcaaa aagattattc
     7141 aaggaaagag ccgttcccac gatccgggtg gggaaaagct ccttgaggtg ggaaatggaa
     7201 aggacgaccc cggatgcgaa gaggccgatg gagaaaaaga cggggaagag aaaaaaattc
     7261 cggccgggcc ccagaaaaaa ggaaagagac agaaggggta ccaggtagat ggaaaggatg
     7321 atcaaggtga gcgttttgcg cgaggggacc agccgatcgg agatcatccc gatgagggga
     7381 cagccgataa tgtagccgaa ggcaatcatg gagaggacgg accccgcctc gaccggggaa
     7441 agctcgaacc cgtccatcag aaacggccct ccccacaagc cctggaatgc gagaaacgaa
     7501 ccggcggtaa aaaaattgag agtggcgatg aaccaaaagg tgggggttcc cagaacagcc
     7561 cggaaggctt ttcccaggga gaaatgcctg ggagaaagga ccggctggtt cgcaacccgg
     7621 gccgggcggt cccggatgac gacccaggcc agaacggcca gaagaagatt gagcgccgcg
     7681 aagagatgga aggacccccg ccaacccagc gaacccgcca gaagagccag gggaatcgtg
     7741 gcggtcagtg ctccagcatt gcccagggag gaaacaatgc cggtgagggt ggcgaattgc
     7801 cggggcggat accagttggc cacgatagtt aaagttccca tgtacatgca cgacattcct
     7861 agcccggtta gccccctgcc cagcaaacag acctcgtaag aaggcgccag ggcaaaggtc
     7921 aggctcccca gcgctcccag catacccagg accgacacca ccttgcgggg gccgatgaag
     7981 tccagagccg gccccagggg gatctgggca acggcgaaaa cgatgaagaa aatgctgctc
     8041 agaaggccca gcttctccgc ccccagggaa aaatcccgca gtaaatgggg agagatcact
     8101 gccggcgagg tgcggtagaa gaaggagaaa aagaaaaggg aaatcatgac cgcaagcatt
     8161 gcgttgcggc gactcatgga ggacctccag gggaatcata acctattttc tcagtaggta
     8221 cacgaagaaa gaagcccagg atggtgcggc ggccaggcgc gcctttaaaa atttaatctg
     8281 gctttttgag gggaaaatag taaactcatt ttttggagga aatcacatgg ctgaaccggg
     8341 ggaagccccc caaaagatcg attatcgggt tctgaccctc ctttccatgg gccacttggt
     8401 caccgatatt accagcggag ctctaccggc catgcttccc ttcttgaagg aagcgctagg
     8461 cctttcctat gccatggccg ggactatcgt tttggtttcc aatctcatgt cttcagtcat
     8521 tcagcccgcc ttcggctatc ttactgaccg caaatccctg ctctggttcc tgcccctggg
     8581 gtctttttgc gcggcccttt ttctggccct tgtaggatgg gccccaagct actccctggt
     8641 cttggtcatg gttgttttca gcggtctggg ggtggctatc tatcatcctg aagggtggcg
     8701 ggtggccaat ttttttgcgg gggaaaagaa ggccacgggg atgtccatct tcgccgtggg
     8761 agggaacctg gggttcgctc tgggaccctt gatggccact ttcttcataa cccacctcac
     8821 cctgaaagga accaccctgt ttgtaattcc cggggcggcg atcgccagcg tcttcctgtt
     8881 cagccgcttc tggagggtgg acaccacggc gatcaagaaa aaaaccgctt ccgaccccgc
     8941 cctcccggcc ctccgaaaag ccctttaccc catgaccctt ctcctgggca tgatcatgtt
     9001 ccgctcctgg gcccatatcg ggatgctcac ctttatcccc ttcttttaca tcaactatct
     9061 gaagggcgat cccatgctgg ccggcaacct gctcttcgcc ttcctcgccg cgggaacggc
     9121 cgggaccttc atcggcgggc cgctggccga ccgcttcggc cataaaagaa tcattctctt
     9181 cagcctgggc ctcagctgtc ccctctttat cctctttctc ctcagctcgg gaatctggtc
     9241 tttcatctgg ctcaccctgg ccgggctgat cctgatcttc tccttctccg tctccatggt
     9301 catgggacag tccttcatgc cccagaatgt gggactggct tccggtctca tcctgggtct
     9361 ctcttttgga atggggggac tcggggccgc cgtgctgggg ctttttgctg atatctgggg
     9421 ggtcccgaca accctctgga tgatcgcctt cctccccctg ggggctttta tccttgccgc
     9481 tctgattccg tacccgctta aaaaatagtt gctggttgct cgttactggt tgttggttac
     9541 tcgttgctga tacctggttt cttaggtttc cgagttttca accgagaacc agtaaccagc
     9601 aactattctc tacaggggat acttttttcc gttcaaggag aggaagaact ggttttcccg
     9661 ctcatccggg agaatgtggg ggagcaagac ctggtaggca ttacgggaga agcgggcctc
     9721 gaataggccg ttgcgcacct tgcagtaggg aatattcccc ggtccgatcc taagggtctc
     9781 gggggagagt ttttcacggg tttcgtcatt cagggtgatc cagacctctt cttccccagc
     9841 cggtccgcgg gaaacgaatt cgagggtcat caccacaaaa ggagcgtctt ccacttccac
     9901 cgggcattct tcccggccaa cccggaggac aattcgccca gcatcatccc gggtcagact
     9961 gcggcagtaa aggagatacg tcttgcgatg ggtaatctct tctccctgaa aaaaccaccg
    10021 tccctcttta tcgattctca tgaaaggccc ttcactttcc gatgcagaac cggctgaaaa
    10081 tccgttccag aaggtcttcg gatgttgttt ccccgaccac ttctcccagg gcctgcaaag
    10141 ccgctcccaa atccacggat aggaattcga cggagagctt caatctgctt gcttccgtgg
    10201 cccgctccag cccttcccgg gccctttcca aggcctgttt gtgacggagg ttggaaagga
    10261 ggatttcgga ggaagactcc gacgttccct tcagcacccg atcccggatc gcgtcttcca
    10321 agtggctgat cctccgatgg tggagggcgg agacgggaat cagcggtacc cccggcatct
    10381 gtcccgaaag gtcttgcagg ttcacgcgca gggggaggtc gtttttattg atcgccacta
    10441 cggtccgctt atcccggact tccctcaaaa tgtccagatc gtcgggagtg aggggctcag
    10501 acccatcgac cacccagatc accaggtcgg cctgggccag ccggtcccaa gtgcgccgta
    10561 ccccttctcc ttccaccgca tccttcgtct ggtggagtcc ggccgtgtcc atgagcttca
    10621 gaaaaatccc ccggacattc actccttctt cgatgacgtc ccgggtcgta cctggaaagg
    10681 gagtcacgat ggcccggtcc tcccgcagaa gggcattgag caggctggat tttcccacgt
    10741 tgggccgccc cacgatgacc gctgaaacgc cttcgcggta gaccttgcct tgctcgtagg
    10801 tttgcagaag ggagttcagc ctttccagag ctccctgcaa ctggaggtga atgttttctc
    10861 cggagagttc gggaacttca tcttcgggga aatcaatgct ggcttccatc tgggccagga
    10921 ggtccaacag tcggtcgcgg acctgacgga cctcggtccc caaccgcccc tggcgctgga
    10981 ggttggccat ttccagggat tttcgggtct tggatcgaat caagtcaatg accgcttcgg
    11041 cctgagtgag gtcgatccgg ccgttgagga aggctcgctt ggtgaactcc ccgggctcgg
    11101 ccaggcgggc tccgctctgc aggaccgcat tcaggatttc ctggaggatc aagataccgc
    11161 tgtgggcctg gatttccacc acatcttccc gggtgtaggt ccggggcttt tccatgaaaa
    11221 ccgccaggac ttcatcgatc accgcccggc cctgggggtg aaggacctcc ccgaggtaaa
    11281 aatggtggga aaggagggga ccgtctgctc ctcggcgacg gaagattttc tcgaagacct
    11341 gccgggcttg ggggccgctg aggcgcacaa ttccgatccc cccaaccccc aggggggtgg
    11401 agatggcagc aatcgtgtcc cctggattca tgaagatttc ttttcggggc tgatgatcac
    11461 tttgcggtac agtccggagc ccgtggacca ggtgcgcagg gtcttgtcgt cctgcagggc
    11521 caagtgaatg atacgccggt cgtgggcact catggggctg atggttaccg ggcggcccag
    11581 gcgcttggct ttctccgcca accgttgggc cagttgaacc agggaatctt ctctccggag
    11641 gcgatagttc tcggtatcga ccacgatcct ctttttgtct tccgcacctt tatggacgat
    11701 cttgttgatt agatactcga gggcgtccag ggtctgacct ttgcgcccga ttagaagccc
    11761 gctgccgtct cccttgatgt tcaggaggat acgttcggat tcctccttca ggtcaacggt
    11821 agcttccacc tccaggagac gaagaatatc ctgcaggatt ttcttggcgg tctcggccgc
    11881 ctggggatcg gagaccgccc ccaaaggacg ggcttcggat atccggggcg cagacttctc
    11941 ctttaccgta gcgcggattc tggcattctt ggcccccatt cccaggaatc cgctggaacc
    12001 gttggaaagg acctcgattt ccaactcttg ccggggagct cccagttctt cgcaggcttt
    12061 atgaatcgct tcctccgtgc ttttgccctc agtttcgaca gacttcatgg gttgcctcgc
    12121 atctatttga tattccggtt catgtaaagc tgctgcccga tggagagcag gttgctgaag
    12181 a
//
LOCUS       DASPRR010000191        11999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1173222, whole genome shotgun sequence.
ACCESSION   DASPRR010000191 DASPRR010000000
VERSION     DASPRR010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11999)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11999
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173222"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VEL68_07455"
     CDS             complement(<1..894)
                     /locus_tag="VEL68_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35837.1"
                     /translation="MLRFALRRILQFIPTILAITLILFILLNILPGDAALMSGDAQDK
                     GVDPRFIEEMKKRWGLDQPIHIRYLTYLKNLAAGDMGLSYLRREKVSGLIASRLWPTL
                     RLAFAAMLIAVSIGIPLGFYSALRQGTWLDAFSMVGAVSGVSIPQFWLGLLLMFFLAV
                     KLPFFPVSGYGDGDLKHILLPAFTLGVGYMALLARTTRAAVVEILTTDYIRTARAKGL
                     SDFLVNRKHILRNALILVLTTAGLQFGSLMGSTVVVEKLFSWPGLGSLMVDSIFQRDI
                     PVTQGCVLLIILVFLLVNLAVD"
     gene            complement(992..2599)
                     /locus_tag="VEL68_07460"
     CDS             complement(992..2599)
                     /locus_tag="VEL68_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35838.1"
                     /translation="MRKALMSMSVILLLGFMAGETLAARSGGTFHFVAPYDGDLLTLD
                     PHKGTRINDMVVMLSLHRSLYSWDPENNRPKLELGEKADVSPDGLVYRIHLKKNVKFH
                     NGRRMTADDVIWSYERVMAPKTVSGSARFVRVIKGAKDYEDGKADKIAGLRKIDDSTL
                     EITMENPVDPAYTLYEVGTGILPREEVEKRGDSFGSDPVGCGPFKFVKWVKGSEIVLV
                     KNPDYYEKGKPYLDKLVYKIMAEAATRDLAFRAKELDATVVGSTQYPVYKADPQFSKN
                     MVEVAELFTRLMGFNPKFEPFAKKQVRQAINYAIDSRLIIQRLLKEKAFPCVGYLPST
                     SPAFDPQAKGYEFNLPKAKALMKEAGYEKGFTFECIGSNSEAFGVVAVEAILPFLKQI
                     NVMVKPQQLEGAALADRVRKMDYQAFIWSLGSGAGGDPLQALNRWYSKNPSTAGNFVA
                     YNNPEFDKLLDTASQIREMAKRVDLLRKADAIFREDAPVWFFNYNKAVVAHQPWVHGI
                     QAVAIEMMYQNMADVWIEESSPRAKEK"
     gene            2734..3567
                     /locus_tag="VEL68_07465"
     CDS             2734..3567
                     /locus_tag="VEL68_07465"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ35839.1"
                     /translation="MEHREGTFEGAGGLELYYQTWRAEKKSLAVLAIIHGFGEHSGRY
                     TNVVNHLIPKGYAVFGFDLRGHGRSPGQRGHINSWDEYREDVRAFLQMTSQREPNQPV
                     FMLGHSMGALIALDYVLRDSAGLRGAIISGAPLEPVGVAKPFLVMLARVLSRVWPRFS
                     LPLGLDPKGISRDPDVVKAYEADPLVHGKTTVRWGTEILQTIEWAKAHAAEVNIPILL
                     IHGGSDPLNSPDGTRNFFEKVISPDKEMKVYPGSYHEAHNDLGYGQVMNDIAQWLERH
                     R"
     gene            complement(3957..4937)
                     /locus_tag="VEL68_07470"
     CDS             complement(3957..4937)
                     /locus_tag="VEL68_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35840.1"
                     /translation="MKKILGIVLLLVLFYPLEAFGHHGGVSLPFGPGTPIETASPLTL
                     PQGGVVTYARVEQVEWRKFDFAEPNNKSSFTFMSAGVSYGFTPYLTGTFSVPYNIKRQ
                     DSFGSNSGLGDIRFSGILGFNYSNGKGLSLNTEKEAAISLEKVKKTFFSIYGGFTLPT
                     GPYKQELGGTVDPAMQPGFGSPSFTLGINAGRAIYGSLSLTADISMDYFTQRDNFKFG
                     NEYRFNLAAVQELYGNTQRFIAKIDGVLELNLLHISRDQEGGEGQRATGGTILYVTPG
                     MRFSFPQIQNANLGIAIKLPILKSLNEQGEQQGAEGLEKYRAIATLSFYF"
     gene            complement(4949..5437)
                     /locus_tag="VEL68_07475"
     CDS             complement(4949..5437)
                     /locus_tag="VEL68_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35841.1"
                     /translation="MKTMGVMVFLLCLSLPPGESWGHSLNYHIEQKGIAVRAFYTEQD
                     PASYSEYEIYGPGDRDTIPHQTGRTDRNGFLAFVPDKAGIWKIRLWGESSHGYHGFTA
                     EVKVDKEFNLESFSKPLVATHTKLITGLSLIFGVFGIYAYLKSRRGEGEKGQSAMGKG
                     EN"
     gene            complement(5434..6153)
                     /locus_tag="VEL68_07480"
     CDS             complement(5434..6153)
                     /locus_tag="VEL68_07480"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HXZ35842.1"
                     /translation="MKTKPYRNGLGSFIFYALLLALVPGAWAHDFWVEQSGKDLLLIF
                     GHGDQREEFDISRVTAIKAFSSGGGEIEVRKEKKGKGLLLQPGEPPSWVWVGIDNGYW
                     SKTIYGWKNLPRRKASRVVEANRSICYAKALLSWNDALLRPAGEALLDIALLENPWGM
                     KAGDLLPFKVFFRGKATPGIPVEGRDHETVATTDKDGNGRVRMDKGRHLLSVSYREPA
                     KDDPDADFITYAATLTFEVGK"
     gene            complement(6398..7339)
                     /locus_tag="VEL68_07485"
     CDS             complement(6398..7339)
                     /locus_tag="VEL68_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXZ35843.1"
                     /translation="MASILVFSERANLALELLGKAKELGGQMGLEVVAAAFNPADAEP
                     YRRRSASKVFYSDQAELKDFEASVYSQALAQIAQKVGASLILLGSTRRGKELAGRLAQ
                     RLRAGCLTDVKSLDWNEGKMRCVRNAYGGATEATQVIATQVQVIALMPRAYEPAPEGE
                     KKGEIITLKLDLKPSRIKVLKKSEKDTGAADIQGAEILVCVGKGLARQEDLKMIEDLA
                     QTLGGLVACTKPLATDLKWLPESRIVGLSGKIGKPRLALCLGISGQVQFSVGIREAKT
                     IMAINTDPNAYIFQITDYGLIADLHQAVPQLTAAMMG"
     gene            complement(7341..8114)
                     /locus_tag="VEL68_07490"
     CDS             complement(7341..8114)
                     /locus_tag="VEL68_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXZ35844.1"
                     /translation="MNIIVGVKQTVDLQQVRIRKETRQPVLENIPLTLGAIDKNALEE
                     AVRLRDKIGGKVFVVSVGPATLEETIKEALAMGADEAVLVTAPELDGAESAKAALLLA
                     GMIKQMEGYDLILLGEGSADNYSGQVGPRLAEILGLPQVTYVKSLEVDGRKVKAVRSL
                     EDILEEVEVEMPALISVVSEINEPRIPAVTQILRAGRKPRKILKPADLAVQPAGGRWV
                     ETLSNLAPVQERKGILFKEGLKELPGLVLALKEEGVGRK"
     gene            complement(8190..8456)
                     /locus_tag="VEL68_07495"
     CDS             complement(8190..8456)
                     /locus_tag="VEL68_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ35845.1"
                     /translation="MKTKLGKNVFKPGKEKHISIRPGFEKDPRLKAAIYACPAGLYSE
                     KSNGEVELDIDGCLECGTCRIACGTEVLDWSYPVGGVGVQFRFG"
     gene            complement(8471..9757)
                     /locus_tag="VEL68_07500"
     CDS             complement(8471..9757)
                     /locus_tag="VEL68_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ35846.1"
                     /translation="MEKFEVVVVGAGLAGLSAAYTLAAEGVEVVVIERGDYPGAKNMT
                     GGRLYTKPLQNFLPELWKEAPFERAVANEIVTLLSPDKSLTLRLRSDEFARPPAHSYT
                     ILRGTLDRWLAKKAEEKGAMLVAKNRVDDLLKRDGKVVGVLAGGEELGADVVIACDGA
                     LSLIAEKAGLRSPGSPNDYAMGFKEVIELPSQTIENRFALKPGEGAAQLFLGDLTKGR
                     FGGGFLYTNRSSLSLGLVIRIKDLAGANPLKEAPLFLDEFKERPEIAPLIAGGEMVEY
                     SAHVISEAGFAGLIQPFADGFLVAGDAAGFSLNMGLTVRGMEFAIASGVLAAQAVKQA
                     REKKDFSATSLAVYQSMLEDSFVLKDLKTFQYAQAFLDNPRLFQHYPQMAGDLLQSLF
                     TIGPDPKQKFSTTIRKKVGWTETWAILKDLRKGLKI"
     gene            complement(9814..10950)
                     /locus_tag="VEL68_07505"
     CDS             complement(9814..10950)
                     /locus_tag="VEL68_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35847.1"
                     /translation="MDFKLSKEQELIQKTARDFSEKYIMPIADQIDRENKVPPEVYKK
                     MFELELFGIPFSEEYGGSGAGYSCYPLAVEQIARGSGGVALTLSVNSLALSAMNLFGT
                     PEQKKRYMPPCCRGEKIASFAFTEPGTGSDPKQIATTAVKDGDSYVINGTKRFISCAD
                     MQGPIVIFAKDNETNFPTGFIMDKFCPGYSISEPWHKIGMRGGVMTDVYLKDVRVPAD
                     SILGPKGQGYWVLQAGIAYGKVGVSSGALGGMQAALDLSIKYAKEKQHRDKTIAKFQG
                     VQMTIAKIAEKTEAARWLVYRLGYLAETLKDPRAFAKEAALTKSFVTEAGVDVSRLAI
                     AVHGSYGLMEDYAVTRIYRDMIIGEQVEGVMDLQRVITASLLLG"
     gene            11248..>11999
                     /locus_tag="VEL68_07510"
     CDS             11248..>11999
                     /locus_tag="VEL68_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ35848.1"
                     /translation="MGSTTINDALEYLFIHNTAKQDVRDGKAPVIVRGDGDYVFDEGG
                     NKYLDLMAGNTRPNAIGYGREEVAKAMYDQAVKLHYFTPGFYITTPAVQLAKKLASIY
                     PGELKTTCFVCDGSEAVESAFKIAKQYHYYANKQRRFKIVSRQMAYHGQTMGALSALG
                     FLNPMRNVMAPLVPGHAFLIPPYCYRCPINLTYPACNLACADALESLIHFEGPDLIAA
                     FIAETVMQGMGALPPPDGYFDRIRQICNKYGVL"
BASE COUNT         2588 a         3485 c         3165 g         2761 t
ORIGIN      
        1 gtccacggcc agatttacca gaaggaaaac cagaatgatc aacaggacgc aaccctgggt
       61 gaccggaata tccctctgga aaatggaatc caccatcagc gagccgaggc cgggccagga
      121 aaaaagtttc tccaccacca ccgtacttcc catgagcgag ccgaattgca ggcccgcggt
      181 agtcaggacc aggattaaag cattgcgcag tatgtgtttg cggtttacca ggaaatcgga
      241 gagtcctttt gcccgggccg tgcggatgta gtccgtggta agaatctcca cgaccgccgc
      301 ccgggtggtc cgggccaaga gggccatata cccaactccc agggtgaaag cgggcaaaag
      361 gatgtgcttc aagtccccgt ccccgtagcc cgagaccggg aaaaagggga gcttcacggc
      421 gagaaagaac atgagcagca gccccagcca aaactggggg atggatacgc cggagaccgc
      481 cccgaccatg gaaaaagcgt ccagccaggt gccctggcgc agggcggaat agaaacccag
      541 gggaatgccg atgctcaccg cgatcagcat cgccgcgaag gccagccgga gcgtgggcca
      601 caggcgggat gcaatgaggc cgctcacttt ttcgcgccgc aggtaggaaa gccccatgtc
      661 tcctgcggcc aggtttttca gataggtgag atagcggata tgaatgggct ggtcaagccc
      721 ccaccgcttt ttcatctcct cgatgaaccg agggtcgacc cccttgtcct gggcatcccc
      781 ggacatgagg gcggcgtccc cgggaaggat gttcaacaaa ataaagagaa tcagggtgat
      841 cgccagaatg gtgggaatga actggagaat tcggcggagg gcaaatctca gcatttccta
      901 acccggtccc ccctcaatcc cgcccaccgg gcggagttga ggggaggaag ctggtttctt
      961 tcttatttgc cggcaagtcc ggcaggcact gttatttttc cttggctcgg ggagaggact
     1021 cctcgatcca cacatcggcc atgttctgat acatcatctc gatggccacg gcctggatcc
     1081 catgaaccca gggctgatgg gcgaccaccg ccttattgta attgaagaac cagaccggcg
     1141 catcctcccg gaagatggca tcggctttgc gcagcaggtc tacccgtttg gccatctcgc
     1201 gaatctggga ggcggtgtcc agtagcttgt cgaactcggg gttgttgtag gccacaaaat
     1261 tgccggccgt ggaagggttc ttggagtacc accggttgag ggcctggagg ggatcgccgc
     1321 ccgcgccgct gcccagagac cagatgaaag cctggtagtc catcttgcgg acccgatcgg
     1381 caagggccgc tccctcgagc tgctggggtt tcaccatcac gttgatctgt ttcagaaagg
     1441 gcaggatcgc ttccacggcc accaccccga aggcctcgct gttcgaaccg atgcattcga
     1501 aggtgaagcc cttttcgtac cccgcttctt tcatgagcgc cttggccttg ggcaggttga
     1561 attcgtatcc tttggcctgg gggtcgaagg ccggcgaagt ggacggcagg tagcccacgc
     1621 aggggaaggc tttttccttg agaagtctct gaatgatcag gcgggagtcg atggcgtaat
     1681 tgatggcctg acgcacttgt tttttcgcaa agggttcaaa tttgggattg aaccccatca
     1741 accgggtgaa aagctcggcc acctccacca tgtttttgga gaactggggg tccgccttgt
     1801 acaccgggta ctgggtggaa cccaccacgg tggcatccag ctctttggcc cggaaggcca
     1861 agtcccgggt tgctgcctcg gccataatct tatagaccag cttgtcgaga tagggcttcc
     1921 ccttttcgta gtaatccggg ttcttgacca gtacgatctc gcttcccttg acccatttga
     1981 cgaatttgaa agggccgcac cccacggggt ccgaaccgaa gctgtcgcct ctcttctcca
     2041 cctcttcgcg gggcaatatg cccgttccca cctcatatag ggtgtaggca ggatcgacgg
     2101 ggttctccat ggtgatctcc agggtggaat cgtcgatttt cctcaggccg gcgatcttat
     2161 cggctttacc atcttcataa tccttggcgc ccttgatcac ccggacgaaa cgggccgagc
     2221 ccgagacggt cttgggagcc atgacccgct cgtagctcca gatgacatca tccgcggtca
     2281 tcctgcggcc gttgtggaac ttgacattct ttttcaaatg aatccggtac actagaccgt
     2341 ccggtgatac atcggctttt tctcccagtt ccagcttggg acggttgttt tccgggtccc
     2401 agctgtaaag gctgcggtga aggctcagca tgactaccat gtcgttgatg cgcgttccct
     2461 tgtgcgggtc gagggtcaga agatccccat cgtaaggggc aacgaaatgg aaagttcctc
     2521 ccgacctggc agccagggtt tccccggcca tgaatcccag aaggagaatg acggacatgg
     2581 acatcaatgc ctttctcata aggcccctcc cttcccttct taaacaaatt ccagtcgaca
     2641 gagacaattg taaagattac tactataagg tatcttttct gtcaatggca catcagtagg
     2701 ttcccaagaa gtcttgttgt tttaggagga ccgatggagc atagagaagg tacttttgaa
     2761 ggggctgggg ggctcgaact ttattaccaa acttggcggg cagagaagaa atccttggcc
     2821 gttttggcca ttattcacgg gttcggagaa cacagtgggc gatacacaaa tgtggtaaac
     2881 cacttaatcc caaaaggata tgcagttttt ggattcgacc tccgggggca cggtcgctcc
     2941 ccgggtcaac gggggcatat aaattcctgg gatgagtaca gggaagatgt aagggccttc
     3001 ttgcaaatga cgtcccaaag ggaaccgaat cagccggttt tcatgctggg acacagcatg
     3061 ggcgccctga tcgccctgga ttatgttctc cgtgattcgg cagggctccg gggcgccatc
     3121 atcagcgggg cgccccttga acctgtcggg gtcgccaagc cattcctggt gatgctggcc
     3181 cgcgtcctgt cgcgggtttg gccccgcttt tcattgccct tgggacttga ccccaagggg
     3241 atttcccgag acccggacgt ggtcaaagcc tacgaagccg accctttggt tcatgggaag
     3301 accacggtcc gatgggggac ggaaattctc cagaccatcg agtgggcaaa ggcccatgca
     3361 gcggaggtga acattcctat tctcctgatc cacggcggtt ccgatcccct gaattcaccc
     3421 gatgggaccc gaaacttttt tgagaaggtc atttcccccg ataaagaaat gaaggtctat
     3481 ccaggaagct atcacgaggc tcacaatgac ctgggctatg ggcaggtaat gaatgatata
     3541 gctcagtggc tggaacggca caggtaaccc caggttggtc ctttaagcaa gagtgtaatc
     3601 ttttcatgga tctccagcga tggatccttt ctctcatctt cctatagaca gctatcacct
     3661 ttattgggat cccggctatc cctgggatgg ttaccttcat tcgtaaaggt caggaatggg
     3721 cgcgaaggaa ccggtatgcc cggctgagcc tacaaatacg cctgtcgagg cttgtcaaat
     3781 cattgatcaa gaaagtgaaa gcggccccaa ggctaaggaa gggaacctgc cgctggtgga
     3841 ggccaagccc agaggggaac catgatcgca tagagcgccc catatgtttg cggccctaaa
     3901 taaccatcgg gaatatgcat ggctcctctc aggagccccc tacgggagcg gagtcttcaa
     3961 aaatagaagg aaagagtggc aatcgctcga tatttttcga gtccttcagc cccttgctgt
     4021 tcgccctgct cgttaagact cttaaggatg ggaagtttta tggcaattcc aagattggca
     4081 ttctgtatct gggggaagga gaagcgcatc cccggcgtta cataaaggat cgtccccccg
     4141 gtcgccctct gcccttctcc cccttcttgg tcgcgggaaa tgtggagaag gttcaattcc
     4201 aggaccccgt caatcttggc gatgaacctc tgggtattcc cgtacagttc ctggacagcc
     4261 gccaggttga atcgatactc attgccgaac ttgaagttat ccctctgggt gaaataatcc
     4321 atggagatat ctgccgtgag gctcagggat ccatagattg cccggcccgc atttattccc
     4381 agggtgaagg aagggctccc gaagccgggc tgcatggcgg gatcgaccgt tcccccgagt
     4441 tcctgcttat agggtcccgt gggaagggta aaccctccgt aaatggagaa gaaagttttt
     4501 ttgaccttct ccagggatat ggcggcttct ttttcagtgt tcagacttaa acccttgccg
     4561 ttggaataat tgaaacccag gatgcccgaa aagcggatgt cccccaggcc gctgttgctt
     4621 ccgaaggaat cttgcctctt gatattatag ggtactgaaa aagtgcccgt gaggtagggg
     4681 gtaaatccgt aggagacccc agcactcata aaagtaaagg aactcttgtt gttcggctcg
     4741 gcaaaatcga atttgcgcca ttccacctgt tcaaccctgg cgtacgtcac tacgcccccc
     4801 tgcggtagag ttagagggga agcggtttca atgggagtgc cgggcccaaa gggcaggctc
     4861 accccgccgt gatgcccgaa ggcttccaag ggataaaaca ggaccagcag gagcactatt
     4921 cccaggatct ttttcatttt tcccttcctt aattttctcc cttccccatg gctgattgac
     4981 ctttttcccc ctcccctctt ctggatttca agtaggcata aattccgaat acaccgaata
     5041 ttaagcttaa gccggtaatt aacttggtat gggtggcgac cagaggcttg ctgaaagatt
     5101 ccaagttgaa ttccttatcc acctttactt ctgcggtgaa gccatggtac ccatgagagg
     5161 attctcccca tagtctaatt ttccagatcc cggctttgtc cggcacgaag gccaggaacc
     5221 cattcctgtc ggttcgcccc gtttggtgag gaatggtatc ccgatcccca ggcccgtaaa
     5281 tttcatactc tgaatagctg gctgggtcct gctcggtata aaaagccctc acggcgatcc
     5341 ctttttgctc tatgtggtaa ttcagggaat gtccccaaga ctcccccggg ggtagactca
     5401 ggcacaaaag aaaaaccatg acccccatcg ttttcatttc cccacctcaa acgtgagcgt
     5461 agcggcatag gtgatgaagt cggcatccgg gtcatccttc gccggttccc tgtagctcac
     5521 cgagagcaaa tggcggccct tgtccatccg caccctgccg tttccatcct tgtcggtggt
     5581 agccaccgtt tcatggtccc ggccttcgac gggaatcccc ggggtggcct tcccccggaa
     5641 aaagacctta aagggcaata gatcccccgc tttcattccc caagggtttt ccagaagggc
     5701 aatgtcgagc agggcctcac ccgcagggcg cagcagagca tcgttccatg ataatagagc
     5761 tttggcgtag cagatggagc ggttggcctc cacgacccgg ctggctttcc tcctgggaag
     5821 gtttttccag ccataaatcg ttttggacca gtacccatta tcgatcccga cccataccca
     5881 cgacgggggt tcaccgggct ggaggagtaa tccttttcct tttttctcct tgcggacctc
     5941 gatctcccct cctccggagg aaaaggcctt tatggctgtt actctggaaa tatcgaactc
     6001 ttccctttga tcgccatggc cgaaaattaa gagaaggtcc tttccgctct gctcaaccca
     6061 aaaatcatgg gcccaagccc ctggaaccag ggcaagcagc agggcataga agatgaatga
     6121 gcctaaaccg tttcgataag gcttggtttt catttccacc ccgctaaact atttttgtgt
     6181 tacgatattg tcttttgtgt cacttgtcaa taaaaaattt tactctccct tcccctccgg
     6241 gatcgagaaa agcttccccc gctttgctcc cttcgccccc tctcgccacc tctgtcccga
     6301 aaatcgcccc caaccctccc gaaaaaaaac cctcaaccaa acccaagacc taccctccac
     6361 aaaacgattt cttaacgact ttttatcaat tttttgatca ccccatcatg gctgcggtca
     6421 attgggggac agcctggtgc aggtcggcga ttaggccgta atcggtgatc tggaaaatat
     6481 aggcgttggg atcagtgttg atggccatga tggtcttggc ctcccgaatt cccaccgaaa
     6541 attgaacctg gccggagatt cccaggcaga gggccaggcg gggttttccg attttaccgg
     6601 aaagccctac gatccggctt tccgggagcc acttcaggtc ggtagccagg ggtttggtgc
     6661 aggccactag tccccccagc gtctgagcta ggtcttcgat catcttcaaa tcttcctgcc
     6721 tggctagacc ttttcccacg cagaccagaa tttccgctcc ctggatatcc gcggcccccg
     6781 tatctttttc cgatttcttc aacactttaa tcctggaggg tttgaggtcc agcttcaggg
     6841 ttatgatctc ccctttcttc tctccctcag gagccggctc ataagcccgg ggcatcaggg
     6901 caatgacctg gacttgcgtg gcgatcacct gcgttgcctc cgtggctccc ccgtaggcgt
     6961 tacgcacaca cctcattttt ccctcgttcc aatcaagcga ctttacatcc gtgaggcagc
     7021 cggctcgcag cctctgggcc agccttccag ccagttcctt gccccggcgg gtagagccga
     7081 gaaggatcag ggaagcacct accttttgag ctatctgagc aagggcttgg gaataaaccg
     7141 aggcctcgaa atccttcagc tccgcctgat cggaataaaa cactttcgat gcacttcgtc
     7201 tccgataagg ctctgcatcc gcagggttga aagctgccgc caccacctca aggcccatct
     7261 gccccccaag ctctttggct ttgcccagca attccaaggc tagattcgca cgctcgctga
     7321 aaaccaggat cgaagccata ttatttcctc cccacacctt cttcttttag ggccagaacg
     7381 aggccgggca gttcctttaa gccctctttg aaaaggatcc cctttctttc ctgcaccggg
     7441 gctagattgc ttagagtttc cacccaacgc ccccctgcag gctgaacggc caaatccgcg
     7501 ggcttcagga ttttcctggg ctttcttccg gccctaagaa tctgggtgac ggcaggaatc
     7561 ctgggttcgt tgatttcgct gaccaccgag atcaaggccg gcatttctac ctccacttct
     7621 tccagaatat cttccaggct tcggaccgcc ttcacctttc gtccatctac ctccagagat
     7681 ttcacataag tgacctgggg caagcctaga atctccgcca ggcgcggccc tacctgcccg
     7741 gaatagttgt ccgccgaccc ttctcccagg agaatcaggt catacccctc catctgttta
     7801 atcatcccgg caagcagcag agctgccttg gcgctttccg ccccatcgag ttcgggtgca
     7861 gtcaccagga cggcttcatc cgctcccatg gccagggcct ccttgatcgt ttcttcgaga
     7921 gtcgccggcc cgaccgaaac gacaaagacc ttccctccaa tcttgtctct caaccgcacc
     7981 gcctcttcca gggcgttctt atcgatggcc cccagggtca ggggaatgtt ctccaatacc
     8041 ggctgccggg tctccttgcg gatgcgaacc tgctgcaggt caaccgtttg tttgacgcct
     8101 actatgatgt tcataaatac gcctccacgg catagagcat aacgcagatc gcaaaactct
     8161 ctactccatg ctccttgctc cctgccgtct tatccaaaac ggaactggac gccaactccc
     8221 ccgaccgggt agctccaatc caaaacctcg gttccgcagg cgatgcggca agtgccgcat
     8281 tccaggcagc cgtcgatgtc cagctcgact tccccattgc tcttttctga atacaaaccg
     8341 gccgggcagg catagattgc cgctttcagc cgggggtctt tctcaaagcc cggacggatg
     8401 ctgatgtgtt tttccttgcc cggcttgaaa acattcttcc ccagtttggt tttcatattt
     8461 ggggtggggc tcatatcttc agtccttttc gtaaatcctt gagtatcgcc caggtttcgg
     8521 tccacccaac ctttttccgg atggtcgtgg agaatttctg cttgggatcc ggtccaatgg
     8581 taaataggct ctgcaagaga tcacccgcca tttggggata gtgctggaaa aggcgggggt
     8641 tgtccagaaa agcctgggcg tattgaaaag tctttaaatc cttgagaacg aagctgtcct
     8701 ccaacatgct ttgatagact gcaaggctcg tcgccgagaa atctttcttc tctcttgcct
     8761 gcttcaccgc ctgagcagca aggactcccg aggcgatggc aaactccatg ccccggacgg
     8821 taagtcccat gttcagggaa aagcccgcgg catcccctgc caccaaaaac ccatcggcaa
     8881 agggctggat gaggccggcg aatcctgctt ccgaaatcac atgcgccgaa tattcgacca
     8941 tctccccgcc ggcaatcaaa ggcgcgatct ccggtcgctc cttgaactcg tccagaaata
     9001 gaggagcttc cttgagtggg ttggccccgg ccaagtcttt gatccggatg accaggccca
     9061 aagaaaggga agaacgattg gtatagagga accctccccc gaatctcccc ttggtcaaat
     9121 ctccgagaaa aagctgggca gccccttctc cgggctttag ggcaaaacgg ttctcaatgg
     9181 tctgggaggg caactcgatc acttctttga atcccatggc gtaatcgttc ggtgagcccg
     9241 gggacctgag tccggccttt tcagctatca aggaaagagc cccatcgcat gcaatcacca
     9301 catccgcccc gagttcctct cccccggcca gaaccccgac gaccttcccg tctctcttca
     9361 gcaggtcatc cacccggttc ttggcgacca gcatggcccc tttttcttcg gcctttttgg
     9421 ccagccaccg atccagagtt ccccggagga tggtgtagga gtgggccggg ggtcgggcga
     9481 attcatcgga ccggagtcgg agagtgaggg atttgtcagg ggaaagcagg gtgacgattt
     9541 cgttggcgac tgccctttcg aaaggggctt ctttccacaa ctcgggcagg aagttttgca
     9601 gcggcttggt atacagccgg ccgccagtca tattcttggc cccgggatag tcgcctcgct
     9661 cgatgaccac cacttcaacc ccctccgcgg caagagtata ggccgcggag aggccagcga
     9721 gaccggcgcc gacaactacg acctcaaact tctccatcgc gggcatccag tatttattgg
     9781 ttcaatccgc aatcgtcaac gcgcactcag cctctaccct aggaggaggc ttgcggtgat
     9841 aacccgctgc aggtccatga ccccttcgac ctgttctcca ataatcatat cccggtaaat
     9901 tcgggttacc gcgtagtctt ccatgagtcc ataggaccca tgcaccgcaa tggccagacg
     9961 ggaaacatcg acccccgctt cggtcacgaa tgacttggtc agcgccgctt ccttggcgaa
    10021 ggcccgcggg tccttcaggg tctcggccag gtatcccaga cggtagacca gccagcgggc
    10081 tgcctcggtc ttctcggcga tcttggcgat ggtcatttgg accccttgga acttggcgat
    10141 ggtcttgtcc cgatgttgtt tttctttggc gtacttgatg gagaggtcca atgcggcctg
    10201 cattcctccc aaggcgccgc tgctgacccc caccttgcca taggcgattc ccgcctggag
    10261 gacccagtat ccctgcccct tgggcccaag gatgctgtct gctgggaccc ggacatcctt
    10321 caaataaaca tcggtcatca ccccgccccg cattccgatc ttgtgccagg gctcggagat
    10381 gctatagccg gggcagaatt tatccatgat gaacccggta ggaaaatttg tttcgttgtc
    10441 tttagcaaag ataacaatgg ggccctgcat gtcggcgcag gagatgaagc gcttcgtccc
    10501 atttattaca taactgtctc catccttgac cgccgtggtg gcaatctgct tggggtcgga
    10561 cccggttccc ggctcggtga aagcgaagga agcgatcttc tccccccggc agcagggggg
    10621 catgtagcgc ttcttctgct ccggagtccc aaagaggttc atggcgctca gcgccaggga
    10681 attcaccgac agggtcaggg cgactcctcc ggacccccgg gcgatctgtt ccacggcgag
    10741 ggggtagcag gagtagccgg cgccgcttcc cccgtactcc tcggagaagg gaattccgaa
    10801 cagctccagc tcgaacatct tcttgtagac ctccggcgga actttattct ctctgtcgat
    10861 ctggtcggca atgggcatga tgtatttctc ggaaaaatcc ctggcggttt tctggatcag
    10921 ttcctgctct tttgatagtt tgaagtccat cccgggcctc ctttggggaa taaatgttta
    10981 ttaccgtcga gtgcaggaga aaaaggacaa aaagacattc agcatccctc tgccaacaca
    11041 ttctttaata gttggaaaag ggatgtcaag ttaaaaaggg gatgagaaca aggtggccgc
    11101 tctcagttgc gaggcaaaaa ggaattcatc cgcttcaacc ggaacccgaa acttggaatc
    11161 tgaagccata ttcttcttaa gcattgacag gcaagtccct ggcttgatac aaaggaattc
    11221 aaacaacttt tgcaggggga gggacaaatg ggttcaacca caatcaacga tgccctggaa
    11281 tacctcttta tccataacac ggcgaagcag gatgtccgcg atgggaaggc tccggttatc
    11341 gttcggggag atggggacta cgtcttcgac gaaggcggaa acaagtacct ggacctgatg
    11401 gccggcaata cccggcccaa cgccatcggg tatggccggg aagaggtggc caaggcgatg
    11461 tacgatcagg cagtgaagct gcactatttc acccctggat tctacatcac caccccggcg
    11521 gtccagttag ccaagaagct ggcctccatc tatcccggcg aactcaagac gacctgtttc
    11581 gtatgcgacg ggtccgaggc cgtggaatcc gccttcaaga tagccaagca gtaccattac
    11641 tatgccaata agcagaggcg ttttaaaatc gtctcccgtc agatggccta ccatgggcag
    11701 accatgggcg ccttgagcgc cctgggattt ttgaacccca tgcgcaacgt catggccccc
    11761 ctggttccgg ggcatgcttt cctcattccc ccctattgct accgctgccc cataaatttg
    11821 acttacccgg cctgcaactt agcttgtgcc gacgctttgg agtccctcat ccatttcgag
    11881 gggccggacc tgattgctgc cttcatcgcc gaaacggtca tgcaggggat gggggcgctt
    11941 cccccccccg atggctattt tgaccggata cgccagattt gcaacaaata cggagtgct
//
LOCUS       DASPRR010000192         7856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1013301, whole genome shotgun sequence.
ACCESSION   DASPRR010000192 DASPRR010000000
VERSION     DASPRR010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013301"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..551)
                     /locus_tag="VEL68_07515"
     CDS             complement(87..551)
                     /locus_tag="VEL68_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXZ35849.1"
                     /translation="MKNFWATLWLAIIILFGSTAAAQKPDAIVGKWWNEEKDAQIEIY
                     PCEGKYCGKIIMLKEPNYPADDSKGMAGKPKIDRESPEASKRERPILGMNIVWGFTHS
                     GENLWEGGFIYNPREGKTYKCKLTLENPDTLKVRGFIGISLIGKTNIWTAVK"
     gene            864..2042
                     /locus_tag="VEL68_07520"
     CDS             864..2042
                     /locus_tag="VEL68_07520"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HXZ35850.1"
                     /translation="MAETQFSFNSLMEITKRPPIVFVEGHGSWLKDSNGKEYLDFVQG
                     WAVTCLGHSPKALVRALSKQAAKLINCSPAYYNETMIKLADLIVKHSCLQRVFFANSG
                     AEANEGAVKLARKWGQKHKNGAYEIITMDHAFHGRTLAMMSASGKPEWKQLFEPKVPG
                     FPKANLNDLNSVRAAINEKTVGVMLELIQGEAGVFVATDEFVKGLRDLAREKGILLIL
                     DEIQTGMGRTGKMFGYEHYGVEPDIMTLAKGLGGGVPLAALVAKENVSCFEVGDQGGT
                     FNGNSLMMAVGIAVFGELTKPRFLGAVSKAGAHLRSRLEEVSKQYGFGEVRGKGLLLA
                     LDLNKEAGPQIVEEAVNQGLLINSPRKNTLRFMPALNVTREEIDRMIDILKRVLQKIG
                     "
     gene            2265..3977
                     /locus_tag="VEL68_07525"
     CDS             2265..3977
                     /locus_tag="VEL68_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXZ35851.1"
                     /translation="MKEHHTIKDLIIEELKTLRKRVQELDKYEEDRQRIHAALRESEK
                     KYQQIYDETPVGYHELDTKGRFTRVNRTELQMLGYSAGEMLGKPVWDFFEEEDTTRKV
                     ILAKLSGDVSFHDTFERTYRRKDGSTILVLVEDRVLRDKGNQIIGIRSMVEDITERRR
                     AEEALRKSEEQLRQWQRVEAIGRLAGGVAHDFNNLLMTIKGCSELLLGAFDRRDPRRE
                     EVEEILKAADRATSLTRQLLAFGRRQILQPQVLDLNAVVMNMDKMLRRVIGEDVQLIA
                     SLRRDLWSVKVDPGMIEQVIMNLAVNSRDAMPNGGKLTIETTNVTHDEEYASHHVSVK
                     PGYYAMLAITDTGCGMDKETQSHLFEPFFTTKEKGKGSGLGLSTVYGIIKQSGGNIWT
                     YSEPGLGTTFKIYLPRVEETSHAYKPEISRKEIKAPGGTETVLLVEDEEAVRSMVSKV
                     LQNKGYRVLEASHGNEALEVCDKFEGPIHLMVTDVIMPQMSGRELAERLALVRPEMGV
                     LYMSGYPDNTIVQHGVLEPGTAFLQKPFTISALELKVREILDSGPPGDRQPQAFEQIS
                     LFHK"
     gene            4332..4541
                     /locus_tag="VEL68_07530"
     CDS             4332..4541
                     /locus_tag="VEL68_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013050629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXZ35852.1"
                     /translation="MEKILTAKEVSQFLKLSESTIYKLAFSGEIPAFKVGDSWRFELE
                     DIQKMIRDSKTRGRRKDSLQDSEKR"
     gene            4798..7035
                     /locus_tag="VEL68_07535"
     CDS             4798..7035
                     /locus_tag="VEL68_07535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HXZ35853.1"
                     /translation="MGDNKKGKKPGAIEQTVEQLAMQTMMVEADDVMALGSILENLEK
                     LSLEDEKAPPAPLCAAIRKLVEKIILSEIPEPQRGLELLRRGVRLIQEGIYHPERPGA
                     MDEEETFRKELEPILGLDLSPPPASKEEGPPASPQEKPPDIAQDMELCSDFTMEALEH
                     LESIELQIINLEQNPEDKECINAIFRPFHTIKGVSGFLNLKEINRFSHAMESLLDDAR
                     NGRLSIHQGIIDFVLEAVDLLKKMIQELREQVKSGQVNPTPVGLEPYLQRISLLRKGE
                     VPPKELLGKGEMPPLGEILSQKGYVSPADIQEALRKQKEEKRDQKIGEILIQENKVKP
                     REVLEALRDQKKGASQVPEAAVKVDTSKLDNLVDLIGELVIAQSLVAQNSVFSGIHDP
                     KLNRDFSQLKRITTDLQRISMSLRMVPIRHTFQKMIRLVRDLAKKSDKQVDLVMSGEE
                     TEIDRNMVDTLYDPLVHMIRNSVDHGIEPQAKRLEAGKPETGKVFLRAFQKGGFIVIE
                     IEDDGQGLNRPKILKKAQETGLVPADASLTDHQIDNLIFEPGFSTADKVTDVSGRGVG
                     MDVVRKTIEKLKGNVEIFSTTGKGCRFVMRVPLTLAIMDGIVVQIGAERYIIPTVFIR
                     ETLRPDRKDLSTVQNRGELIKVRDTFLPLIRLHRTLGIQTKQEHPWEALVVVVENEGR
                     QKGLMVDDLLGKQEVVIKNLGEKLKTVEGVAGATIMGDGRVGLILDIHGIFEIDMNSS
                     SMAQA"
     gene            7401..>7856
                     /locus_tag="VEL68_07540"
     CDS             7401..>7856
                     /locus_tag="VEL68_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXZ35854.1"
                     /translation="MMLQRFKLSTRIMFMGVMIVVCFSLVFAWITPRVKGNLYDAKYE
                     KTKHVVESVWGILDYYGKQAKAKTMPVEEAKKKAMEAVKGLRYDKDEYFWINDTEPRM
                     VMHPIKPEMDGTSLAENKDPHGKRLFVAFVDVCRKNGEGFVDYMWPKPGS"
BASE COUNT         2048 a         1970 c         2275 g         1563 t
ORIGIN      
        1 ggcagcgaag aaaaaatcga tgccagaaga aatggcgccc gattcttctc ctattttcgc
       61 agtgggatgg agctaaaagg cagggattat tttaccgcgg tccagatgtt cgtcttcccg
      121 atcagggaaa tgccgatgaa gccgcggacc ttcagggtat cgggattttc cagggtgagt
      181 ttgcacttgt aggtcttgcc ttccctcggg ttgtagatga atcctccctc ccagagattt
      241 tccccggaat gggtgaatcc ccagacgata ttcataccga ggatcggccg ctcccttttg
      301 gaagcctcgg gactctcccg gtcgattttg ggttttccgg ccatgccctt ggagtcatcg
      361 gccgggtagt tgggttcttt cagcatgatg atttttccgc agtatttccc ctcacaggga
      421 taaatctcga tctgggcgtc cttttcctca ttccaccatt ttcccacgat ggcatccggc
      481 ttttgggcag cggccgttga accgaacaat attatgatcg ccaaccaaag ggtagcccaa
      541 aaatttttca tagtagacct ccggaaattg agattcggta accggaaaga aaaacaagga
      601 agatgaaaac tgccccattt tagggcggag agcggaaaag gtcaagaaaa tgcggagtgc
      661 ggaattcgga atgcggaatg cggaataaca ggagtactgt tgtcttaccg tgccgtaatt
      721 tcaggggaaa agaccgggac ctcgcaattc cagcacggtt tataaatcaa ttgacaagag
      781 ggggatagaa gaataaactc gaacatcgaa agaagaacaa ggggaaatgc ccgcgacaac
      841 tcacctgtag gaaggaaaag accatggcag agactcagtt ttctttcaat tccctcatgg
      901 aaatcaccaa gcggcctcct atcgtttttg tggaagggca cgggtcctgg ctgaaggact
      961 cgaacgggaa ggaatacctg gattttgtcc aggggtgggc ggtcacctgc ctgggacatt
     1021 cgcccaaagc tctggtgcgt gccttatcca agcaagcggc caaactgatc aactgcagcc
     1081 ccgcctacta caacgagacc atgatcaagc tggctgacct gattgtgaag cacagctgtc
     1141 tgcagcgcgt cttttttgcc aacagcgggg ccgaggcaaa tgagggggcg gtcaagctgg
     1201 cccgaaaatg gggacagaag cacaaaaacg gggcttatga aattataacc atggaccatg
     1261 ccttccacgg gcgcaccctg gccatgatgt ccgcctcggg caaacccgag tggaagcaac
     1321 tattcgaacc caaagtgcct ggattcccca aggcgaacct gaatgatctg aacagcgttc
     1381 gagcggccat caatgaaaaa acggttgggg tcatgctcga gctcatccag ggagaagcgg
     1441 gcgttttcgt ggccaccgac gagttcgtga aagggttgcg cgacctcgcc cgggaaaagg
     1501 ggattctttt gatcctggat gagattcaga ccggcatggg ccgcacgggc aagatgttcg
     1561 gctatgagca ttacggggtg gagccggaca tcatgaccct ggccaaaggg ctgggaggag
     1621 gcgttcccct tgccgctctg gtggctaagg aaaatgtctc ctgctttgaa gtgggggatc
     1681 agggaggaac gttcaacggc aattcgctca tgatggcagt gggtatcgcc gtattcggag
     1741 aactgaccaa acccaggttt ttgggtgctg tctccaaggc cggagcccat ctgaggtccc
     1801 gcctggaaga ggtctccaag caatacggct tcggagaagt gcgggggaag ggacttctcc
     1861 tggctttaga cctcaacaaa gaagcgggtc cccagattgt ggaagaggcg gtgaaccagg
     1921 ggttgttgat caattctccg cgcaagaata ccctgcgctt catgcccgcc ttgaacgtga
     1981 cccgcgagga gatcgaccgg atgattgata ttttaaagcg ggttttgcag aagatcgggt
     2041 gataacactt gcccgcaatg ccagaaagga tgagaagtga aaaagcccgg gtttcatgga
     2101 ccgggctttt ttctgggtca aatcgggaat aagggcacga tatcaaaata gttttcaaaa
     2161 aggaaagttg atttttcggg catccatcgg atatactctt gaagggaaaa ggcttttgag
     2221 gccaggttcg ctttccaagg tttccaacgg aagaccctcg cgccatgaaa gaacaccaca
     2281 cgatcaaaga cctgattatt gaagagctga agactctgcg gaaacgggtc caggaactgg
     2341 acaagtatga ggaagaccgc cagaggattc atgccgcgct gcgggagagc gagaagaagt
     2401 accagcagat ctacgatgaa accccggtgg gatatcatga gctggacacc aaaggaaggt
     2461 tcacccgggt caaccgcacg gagctgcaga tgctgggtta cagcgcgggg gagatgctcg
     2521 gaaagccggt ctgggacttc ttcgaggaag aggacacgac ccgcaaggta atcctggcca
     2581 aactctccgg cgatgtttcc ttccacgata ccttcgagcg gacctatcgg cggaaagacg
     2641 ggtccaccat cctggtcctg gtggaagacc gggtcttgcg ggacaaggga aaccagatca
     2701 tcggcatccg ctccatggtt gaggacatca ctgaacggag acgggccgaa gaagccctgc
     2761 gcaagagcga ggaacagctg cgccagtggc agagggtgga agccattgga aggctggccg
     2821 gcggggtcgc ccatgacttc aacaacctgc tcatgaccat caaaggctgc agcgagcttt
     2881 tgctcggggc ctttgaccgg cgcgaccccc ggcgggagga agtggaagag atcctgaaag
     2941 ccgccgaccg ggccacctct ctcacccgcc agctcttggc cttcggccgc agacagattc
     3001 tccagcccca ggtcctggac ctgaatgccg tggtcatgaa catggacaaa atgctccgcc
     3061 gggtcatcgg cgaagacgtt cagctgatcg ccagtttacg ccgggacctc tggtcggtca
     3121 aagtggaccc gggcatgatc gagcaggtga tcatgaacct ggcggttaat tcccgggacg
     3181 ccatgcccaa cggcggcaaa ttgaccatcg aaacgaccaa tgtgacccac gacgaagaat
     3241 acgcaagcca tcacgtgtcg gtgaagcccg ggtattacgc catgctggcg atcaccgaca
     3301 ccggatgcgg tatggacaag gagactcaat cccacctttt cgagcctttt ttcaccacca
     3361 aggaaaaagg gaagggaagc gggcttggcc tctccacggt ctacggaatt attaagcaaa
     3421 gcggcgggaa catatggacc tacagcgagc cggggctggg gactactttc aaaatttacc
     3481 ttccccgagt ggaagaaaca tcccatgcat acaaacccga aatcagccgc aaagaaataa
     3541 aggctcccgg ggggacggag acagttctcc tggtggagga tgaagaagcg gtccgctcca
     3601 tggtcagcaa ggtcctccaa aacaaaggtt acagggtctt ggaagcctcc catggaaacg
     3661 aagccttgga ggtctgcgat aaattcgagg gacccatcca ccttatggtg accgacgtga
     3721 tcatgccgca aatgagcggc agagaactgg ccgaacgcct ggccctcgtg cgtccggaga
     3781 tgggggtcct ctacatgtcc ggctacccgg acaataccat tgtccagcat ggggtcctgg
     3841 agccgggaac cgcctttctc cagaagcctt tcaccatcag cgccctggag ctcaaggtcc
     3901 gcgaaatcct ggattccggc cctccaggag accggcagcc tcaggccttc gagcagatca
     3961 gcctcttcca caaataaata taagatgcca atcttagaag cggaaacctt ctttttgcct
     4021 ccgctcgaga attaagaaaa acatcccacc tgcaaagaaa acctcccaat catcttctct
     4081 tcaccattga gcttctttct ggggattccc caattattgc tgcatttatg ttcgaatatc
     4141 ccgtttaccg aaatcaaacc cttagcctcg attaaagttt tatctagaaa tgccgatatc
     4201 taaaacaaat tggggtaatc agcgcccatt tgaccgtcca ccatccaaaa ttaaagcgga
     4261 agggatagaa aaaaagcagg attcaggact gaagcgtccg cccggggcaa ttcatcggat
     4321 gaggaaggga catggagaag atccttacag ccaaagaggt gagccagttc ttgaagttga
     4381 gcgaatcgac gatttacaag ctggctttta gcggggaaat ccccgccttc aaggtgggtg
     4441 attcctggag gtttgagctg gaggatatcc agaaaatgat ccgggattcc aagacccgag
     4501 gaaggcggaa ggattcgctg caagatagcg agaaaagata atccggccgg ccacaaagac
     4561 cgacgaagga ccctatcatt tctctggaga tcattaaacc agtcaacgac ttttggctag
     4621 ccccttcctt ggaagttcca gcaggaaaga gaaaatctaa tgggctgcgg cctggatgaa
     4681 aaaaaattcc tcaggtgttc cctgcagggc aagggaacag gcgcggagaa ggtttatcga
     4741 cgtttttgat ccctcattga gccctcgaga aggggtggga aatgggaagg gggattcatg
     4801 ggagacaata agaaaggaaa gaaaccgggg gcgatcgagc agaccgtcga gcagctggcc
     4861 atgcagacca tgatggtcga ggccgacgac gtgatggccc tggggtcgat cctggagaac
     4921 ctggaaaaac tgagcctgga ggatgaaaag gccccgcccg caccgctgtg cgcggccatc
     4981 cgaaagttgg tggagaagat catcctgagc gaaatcccgg agcctcaacg aggtcttgag
     5041 ttgctgcggc ggggggtgag gctgattcaa gaaggaatct accatccgga acgcccaggg
     5101 gctatggatg aggaagaaac cttccgaaag gaactggaac ccatcctggg tctcgacctt
     5161 tcacctcccc cggcttcaaa agaagagggt cctcccgctt ccccgcaaga gaaaccgccg
     5221 gacatcgccc aagatatgga gctctgcagc gacttcacga tggaagcgct ggagcacctg
     5281 gagtccatag aattacaaat catcaacctg gagcagaacc ccgaggacaa agaatgcatc
     5341 aacgccattt tccggccctt ccacaccatc aaaggagtct cggggttcct gaacctcaag
     5401 gagatcaatc gattttccca tgccatggag tcgctcctgg atgacgcccg gaacggccgg
     5461 ctgagcattc atcaggggat catcgatttc gttctggagg cggtggacct tcttaagaaa
     5521 atgatccagg agctgaggga gcaggtgaaa tcagggcagg tcaaccccac gccggtgggc
     5581 cttgaaccct acttgcagag aatcagtctt cttcggaaag gcgaggtgcc cccaaaagag
     5641 ctgttgggga aaggcgaaat gccgcccctg ggcgaaatcc tgagccagaa aggatacgtg
     5701 tcgccggcag atatccagga ggccctgcgg aaacagaagg aagaaaaaag agaccaaaaa
     5761 atcggggaga tcctgattca ggagaacaaa gtcaaacccc gggaggtgct ggaggctctc
     5821 cgggatcaaa aaaagggggc ctctcaggta cccgaggccg ccgtaaaagt ggataccagt
     5881 aagctggata acctggtgga tttgatcggc gaactggtca tcgcccaatc ccttgtggcc
     5941 cagaattccg ttttttcggg aatccatgat cccaagctga accgggattt ttcccagttg
     6001 aaaagaatta ccaccgatct ccagcgaatc tccatgtccc tccggatggt gcccatccga
     6061 cacactttcc agaaaatgat ccgcctggtc cgggacctgg ccaagaaatc ggacaagcag
     6121 gtggacttgg tcatgtccgg ggaggagacg gagatcgacc gtaacatggt ggacaccctc
     6181 tacgatcccc tggtgcacat gatccgcaat tcggtcgacc acgggattga acctcaggcg
     6241 aagcgcctgg aggcgggaaa accggaaacg ggaaaagtct ttctccgagc cttccagaag
     6301 ggggggttca tcgtaatcga aatcgaggat gacgggcagg ggctgaaccg gccgaaaatt
     6361 ctgaaaaaag cccaggaaac tgggctggtt cctgccgatg cttcgctgac cgaccaccag
     6421 atcgataacc tgatcttcga gcccggtttt tccactgccg ataaagtcac ggatgtgtcc
     6481 ggccggggag tgggaatgga cgtggtgcgg aagaccatcg agaagctcaa gggaaacgtg
     6541 gagatttttt ccaccaccgg caaaggatgc cgttttgtaa tgcgggttcc cctgaccctg
     6601 gccatcatgg acggaatcgt ggtccagatc ggggcggaaa ggtacatcat tcccacggtc
     6661 ttcatccggg agacgttgag gcccgatcgg aaagacctgt ccacggttca gaatcgaggg
     6721 gagttgatta aagtccgcga tactttcctg cccttgattc gtctccaccg aaccctggga
     6781 attcaaacca agcaggagca tccctgggag gccctggtcg tggtggtgga aaacgaaggg
     6841 cgacagaagg ggctcatggt ggatgatctt ctgggaaagc aggaagtagt catcaagaat
     6901 ctgggggaaa aactaaagac cgtggaagga gtggccgggg ccacgatcat gggggacggc
     6961 cgggtgggat tgatcctgga catccacggg atcttcgaaa tcgacatgaa ttcttcatcc
     7021 atggcccaag cttgaccgga ggcccttgag cctttccctg tctcagaggt caagaaccgg
     7081 gtcccgggcc aattcggttg gaaaaggaaa aaaatgtttt ttcccggcag gcaagtccgg
     7141 ggtcagagaa cctgaaggga gattaggaaa atggctgccc ttttgaaaat gggccctaca
     7201 taccccgctc cttttttgag acgggagggg cggggagttc ttcgtctgaa ataggccccg
     7261 aggggtttgg cttgttgcgg cagatcggat gaaaatgata tcgatcggtt caggacgggg
     7321 ctgtcgatat taataagaag attccccttg agggaaaaaa tgaccgcttt agatcaaacc
     7381 attcaataaa agggagaaaa atgatgttgc agcgattcaa actttcaacc cggattatgt
     7441 ttatgggggt aatgatcgta gtctgctttt ccctcgtctt tgcatggatc actccccggg
     7501 tcaagggcaa cctgtatgac gccaagtatg aaaagaccaa acacgtggtg gaatcggtat
     7561 ggggaatttt ggactattat ggcaagcagg ccaaagccaa gaccatgccc gtcgaagaag
     7621 ccaaaaagaa ggccatggag gcggtcaagg gcctccgtta tgacaaggat gaatatttct
     7681 ggatcaatga tacagagccc cgcatggtga tgcatccgat caagccggag atggacggca
     7741 ccagcctggc ggaaaacaaa gacccacacg gaaagaggtt gtttgttgcc tttgtggatg
     7801 tctgccgaaa aaatggagag ggctttgtgg attacatgtg gcctaaaccg ggttcc
//
LOCUS       DASPRR010000193         6865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1173685, whole genome shotgun sequence.
ACCESSION   DASPRR010000193 DASPRR010000000
VERSION     DASPRR010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173685"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1199)
                     /locus_tag="VEL68_07545"
     CDS             complement(36..1199)
                     /locus_tag="VEL68_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35855.1"
                     /translation="MKIMKTQKWYDSFSPVWIVAVILATILAWVGMENSAGAQDEFKY
                     GSLMPVTGPIPQYGEYFIRGSQLALEDLEKSGWIGGKKIRVVLEDSKADPKVALVAIN
                     KLVNIDKVPIVESLVTPVLMAVGPIAMQNSVVLVNTAAQSVPLRKLGQFFFSMNPLAD
                     VVMSLTVEYAVKGMGAKTAAIIHVNNDYGRLVGQTFKDIFEKQYGGKIVATEIINMGE
                     TDFTTHLTKIKFVQADIIFTVAHEAELGYALKKARQIGLKTPFLSGPGMTGPMTIEIA
                     GEAIEGLKTPDYVFDPYNGTERMKEFGKRYKERFKIMPYSFPAFSYDSVMLYADALKS
                     GARTGPQIRDFYRSLKNWVGVSGPVTFDQDGITTTGAVIREFQKGAFVDVKWK"
     gene            complement(1345..2550)
                     /locus_tag="VEL68_07550"
     CDS             complement(1345..2550)
                     /locus_tag="VEL68_07550"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ35856.1"
                     /translation="MAKGDSNLILEGIRVIDLSRVLAGPFCGAILGDMGADVIKVEDT
                     KTGDELRTWPPHKEGESPGFLVNNRNKRGIAVNMKAPEGVGIIRHLAKGADVLIENFR
                     TGTMEEFGIGYDQLAQINPRLIYCSVSAFGRTGPRAQEAGYEALMQAFTGIMSMTGEP
                     DGPPVRCGVSFLDLTTGILCALGTVSALYQRAQTGLGQRIDGSLLATAVGLMNFQAEG
                     YLLAGLVPKALGSSAPGTSPYRNFRCADNQWVFIAGANDNLWKRLAGAIGLGLLAEDP
                     RYATNVERVKNRVELEKIVQEAIFRFDREPLLKLLEEAGVPATPVNRVDQALEDAQVK
                     AQNMIWHTDLPRLGKTPIVGFPLAFSRMQPRIRRNPPRQGQHTDEILKECGYSAGEIA
                     LLREKKVIL"
     gene            complement(2786..3526)
                     /locus_tag="VEL68_07555"
     CDS             complement(2786..3526)
                     /locus_tag="VEL68_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXZ35857.1"
                     /translation="MVNSVYRAAEILSMLSAGKNRITDIGNELDLSKSTTHRLLKTLE
                     NCGFVFQDPVSHQYFLGHLIIKLSSDPLTTHQNLIVCAYEEMKRLREVSGETVTIHIR
                     IGTQRICLEEMQANQNIRYGVGRGSVSPIYAGSAGKILLSELTEKDRGHLIKNIHLTP
                     VGPHTITDRNGLLREIEKIKKQGYAISSGETLEGAISISVPIKGYFCPVALSIFGPKF
                     RLEPRILSLKKEMERGAKRIGTKIKHAG"
     gene            complement(3614..5143)
                     /locus_tag="VEL68_07560"
     CDS             complement(3614..5143)
                     /locus_tag="VEL68_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ35858.1"
                     /translation="MNFGETLTIQSYRHPDKIAVEDDNKRISYRELNDRVNRLVHGFT
                     ELGLKKGDIICHLQGNTVEHFELLFAVAKAGMVRIPLNPRGEKSEFIKLVNTFEPQAF
                     LYEQEFSGVVREISPYIKCPRFIFVGKTLPLGSIAYDALAVQYPNTEPDIEVEEGDPF
                     LIQSTSGTTGFPKAALISQGGLIRRAIIRAMDLNNHSRGVYLGVTALANTASLFYACS
                     QLYLGGTVILRNRFDPAKTAETIREKQVTHLSMVPVMWEKILRLPGLKVYDFRSLEIA
                     VSYGAPLHQSTKEKFIRDLSPNLFETFGITETGPISNLHPRDQLRKKNCVGQPTMHTQ
                     IRIVDENGRELPPQREGEIVVRTPYLFMGYFKNPEATAEVLRDGWFYTGDIGRLDEEH
                     YLYILGRKKDMIISGGYNVYAEEIEGILASHPKIHESAVIGVPDEEWGESVKALVVLK
                     EGSDASQEEIIEFCKARLADYKKPRTVEFVPSLPRGGPGKIAKNALREKYWAGLERKV
                     N"
     gene            complement(5207..6352)
                     /locus_tag="VEL68_07565"
     CDS             complement(5207..6352)
                     /locus_tag="VEL68_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ35859.1"
                     /translation="MKEVAIIGVGIHKFGRFENKSYLELGQEAAQMALRDANLQWKDV
                     QMAYLSKMYLPATSGARILKPMGATGIPICDIEAACASGGIALSQAILGIQAGAFDIV
                     MVLGTEKMPRGFMDPSMIYEKWQIQMGMDTNPSYWAMRARRHMHEYGTTDLHLAKVAL
                     KNHKNSVHNPYAMYRKEFGIEEIMNSPLVCDPIHLLEICAPNEGSAAVILCPKEKAAQ
                     FTSKPITVAACVPTNAYYSADFRAPVDSLSAKAMNPGPTEVAAKKAYQQAGIGPQEIN
                     CFEVQDTDAFCEIEIYEQLGLCKKGEGGRLLDEGATEIGGKYPVNTSGGLISKGEPIG
                     ASHLGQVFEIVTQLRGEAGKRQVPNAKVGLAHVLGAGNNCAVTILKV"
     gene            complement(6364..6804)
                     /locus_tag="VEL68_07570"
     CDS             complement(6364..6804)
                     /locus_tag="VEL68_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXZ35860.1"
                     /translation="MPQPSSNEKKQIPIKEGLFTAPLSPVERVRLMGSQCRDCSEISL
                     GKRQTCSNCGGGNLTEVPLSRQGKLWTYTVIRHKPPGDYKGPDPFVPFGLGLVELPEG
                     IRVLSPIQCDLERMKVGMELEMEVYPLYTDENLNEVMAFRFGPV"
BASE COUNT         1447 a         1937 c         1747 g         1734 t
ORIGIN      
        1 tgaatcgccc ctacgatttc tccgggtgcc gagcctcatt tccatttcac atctacaaaa
       61 gcgcccttct ggaactcacg aatcacggcc ccggttgtgg tgatcccgtc ctgatcaaag
      121 gtcaccgggc cagagacccc aacccaattt ttcaaagagc ggtagaagtc cctgatttgt
      181 ggcccggtcc gggccccgct tttcagggcg tcggcataaa gcatcaccga gtcgtaggaa
      241 aaggccggga agctgtaagg cataatcttg aagcgttcct tataccgctt cccgaattcc
      301 ttcatgcgtt cggtcccatt gtaaggatca aagacgtaat caggagtttt cagtccttcg
      361 atagcttccc cggcaatctc gatggtcatg gggccggtca ttcccgggcc ggacaggaaa
      421 ggagttttca acccgatttg cctggccttc ttaagggcgt agcccaactc ggcttcatgg
      481 gccacggtga aaatgatgtc ggcctggacg aacttgatct tggtcaggtg ggtcgtaaaa
      541 tccgtctccc ccatgttaat gatctcggtg gcgacgatct ttcccccata ctgtttctcg
      601 aagatatcct taaaagtctg acctaccagc cgtccataat cgttattcac atgaataatg
      661 gccgctgtct tggcccccat ccctttgacc gcatattcca ccgtgagcga catcaccacg
      721 tcggccagag ggttcatact gaagaagaac tggccgagct tcctcagggg gacgctttgg
      781 gcagcggtgt tcaccaggac gacgctgttc tgcatcgcaa tcgggccgac cgccatcaat
      841 accggggtta cgaggctctc cacgatgggg accttgtcaa tattgacaag tttgtttatg
      901 gccacaagag caaccttggg gtctgccttg ctatcctcca gtacgacccg gatcttcttg
      961 cccccgatcc atccgctttt ttccaggtcc tccagcgcca gctggctgcc gcggatgaag
     1021 tactccccgt actgcggaat ggggccggta accggcatca aagaaccata cttgaactcg
     1081 tcctgggccc cggcggaatt ctccatcccc acccaggcca gaatcgtggc caggatcacg
     1141 gccacgatcc ataccggaga aaatgaatcg taccacttct gggttttcat gatcttcatt
     1201 tttcccctcc ttttttatat ttgggacctt ctcccagttc gttggcctga ttgtttgcga
     1261 aaccctccgg gcttcccccc ttcctcttcg cccgactgcc gaaggcgcca ttttcacccc
     1321 tggtaaaacg gggggccttc tttcttaaag aatcactttc ttctcccgca gcagggcgat
     1381 ttcgccggca ctgtatccgc actcctttag gatttcatcg gtatgttgac cctgccgggg
     1441 gggattgcgg cgaattcggg gctgcatccg ggagaaagcc agggggaaac ccacaatggg
     1501 ggttttcccc aaacggggca gatcggtgtg ccagatcatg ttttgcgctt tgacctgggc
     1561 gtcctccagg gcctggtcga cccggttgac cggagtcgcg ggtacccccg cttcttccag
     1621 gagcttcagt aagggttccc ggtcaaagcg gaagatggcc tcttgaacga tcttttccaa
     1681 ctccacccgg ttcttgaccc gctcgacgtt ggttgcatat cgcgggtctt cggccaataa
     1741 gcccaacccg atggcccctg ccaggcgttt ccagaggttg tcgttggcgc cggcaatgaa
     1801 aacccactgg ttgtcggcgc agcgaaagtt gcgatagggg gaggtgcccg gagcgctgga
     1861 ccccagcgct ttgggaacta ggccggccaa caagtatcct tcagcctgaa aattcatcaa
     1921 ccccactgcg gtggccagca gcgaaccgtc gatcctctgc cccagcccgg tttgagcgcg
     1981 ctggtaaagg gcggaaaccg tgcccaaggc gcagaggatt cctgtggtga ggtccaggaa
     2041 ggaaaccccg cagcgtaccg gcggcccgtc gggctcgcca gtcatggaca taattccggt
     2101 gaaggcctgc atgagtgcct catagccggc ttcctgcgcc cgtggcccgg ttcgcccgaa
     2161 ggccgaaacg gagcaataaa tcaatcgggg attgatctgg gccagttggt cataaccaat
     2221 cccaaactcc tccatggttc cggtgcgaaa attttcgatc agcacgtcgg cgcctttggc
     2281 caggtggcgg atgattccca ccccttcggg cgctttcatg ttgaccgcga tcccgcgctt
     2341 attacggttg ttgaccagaa agcccggaga ttccccctct ttgtggggcg gccaggtacg
     2401 aagttcgtcg ccggttttgg tgtcttccac tttgatgacg tcggctccca tgtcacccag
     2461 aatggcgccg caaaagggcc cggccagcac cctggataga tcgattaccc gaatgccttc
     2521 cagaatcagg ttggagtctc ctttggccat gagtcaagct tcctttcgca ggacgagtca
     2581 atttttctga atggtcactg tcagcccaaa tcgttttttc tatttcccct aagatgaata
     2641 gacattgagc ttattcctag ataaggtcct ggatttaaat ccccctttaa aaaaggggga
     2701 tatgggggat ttgttttaaa tcaccttgga aaaatccccc caacccgcct ttataaaggg
     2761 gggaatatta tttacgcata aattcttaac cggcatgctt gattttagta ccgatccgct
     2821 tggcgcccct ttccatttct tttttcaaac tcaaaattct cggctcaagc cggaatttgg
     2881 ggccaaaaat gcttaaggcg acgggacaga aataaccctt aatggggacg gatatcgata
     2941 tggctccctc cagcgtctcc cccgagctga tcgcatatcc ctgctttttt attttttcta
     3001 tttcccgaag caacccattt cgatccgtta tggtatgggg tccgaccggg gttaaatgga
     3061 tattcttgat caggtggcct cggtcttttt cggtgagttc cgataataaa atcttcccgg
     3121 cagacccggc atagatcggc gacaccgacc ctctgccaac gccgtagcgg atattttgat
     3181 tggcctgcat ttcctccaga caaatcctct gagtcccgat gcgaatgtgg atggtgaccg
     3241 tctccccgct gacctcccgt aatctcttca tttcttcgta agcgcatacg atcaggttct
     3301 ggtgcgtggt cagcgggtcc gaggaaagtt ttatgatcag gtgtcccaaa aaatattgat
     3361 gggaaaccgg gtcttgaaaa acgaatccgc aattttccag ggtcttcaac agacggtgag
     3421 tggtgctttt acttaaatcc aattcgttgc caatatcggt aatccggttt ttgcccgcgc
     3481 ttaacatgct caagatttcc gcagcccgat aaacagaatt aaccatgaat atcctcagga
     3541 ccttgaaaga ttaaactggg gaagcctggc acagcccgtc catctcccct tgattttttc
     3601 catgttggga aaatcagttg acctttctct ccagtcctgc ccaatatttc tctcggagcg
     3661 catttttggc tattttcccg ggaccgccgc gagggaggct gggcacgaat tcaacggtcc
     3721 ggggcttttt atagtcggcc aggcgggctt tgcagaattc aatgatctct tcctggctgg
     3781 cgtccgatcc ctctttcaaa acgaccaatg ctttcaccga ttccccccat tcctcatcgg
     3841 gaaccccgat gaccgccgac tcgtgaatct tcgggtggga ggccagaatt ccttcgattt
     3901 cttcggcata gacgttgtaa ccgccgctga tgatcatgtc cttcttccgg ccgagaatgt
     3961 ataaataatg ctcctcgtct agccttccga tgtctccggt ataaaaccat ccgtctcgaa
     4021 ggacttctgc ggtagcctcc gggtttttaa agtaccccat gaataaataa ggggtccgga
     4081 ccacaatttc accttccctt tgggggggaa gctcccggcc gttttcatcc acgattctaa
     4141 tttgggtatg catggtaggt tggccgacgc aattcttctt tcttaactgg tcgcggggat
     4201 gcaggttgct gattgggcct gtttcggtga tcccgaaggt ttcaaaaagg ttgggtgata
     4261 aatcccggat gaatttttct ttggttgact ggtgcagagg agcaccataa gagacggcga
     4321 tctccaggga gcgaaagtcg tacactttca atccgggaag tcggaggatt ttttcccaca
     4381 tgaccgggac catggaaagg tgggtcacct gcttttcccg gatggtttcg gcagtttttg
     4441 ccggatcgaa gcgattccga agaatgaccg tacccccgag gtacaattgg ctgcaggcat
     4501 aaaagaggga ggcggtgttc gccagggcag taacgcccaa atagactccc cgggaatgat
     4561 tgttcaggtc catggcccga atgatggccc ggcgaatcag ccccccttgc gaaatcaggg
     4621 cggcttttgg aaaacccgtg gtccccgagg tggattgaat gagaaatggg tcaccctctt
     4681 ccacctcgat gtccggctca gtattcgggt attgcaccgc caatgcgtcg taggcgatgg
     4741 agcccaaggg gagggtcttt cccacaaaaa tgaaccgggg gcatttgatg taggggctga
     4801 tttcccggac gactcccgag aattcttgtt cgtaaaggaa agcttgaggt tcaaaggtat
     4861 tgacaagctt gatgaactcc gacttttctc ccctggggtt aagaggtatt cgaaccatgc
     4921 ccgctttggc cacggcgaac aggagttcga aatgctcgac cgtattccct tggagatgac
     4981 agatgatatc gccctttttt aaccccagct cggtaaaccc gtgaaccagg cgattcaccc
     5041 ggtcgtttaa ctcccgataa gaaatcctct tgttgtcatc ctcgacggcg attttatcgg
     5101 ggtggcgata ggattgtatg gtcaacgttt caccgaaatt caatccgctc tcctttgccc
     5161 ccgaacctct tgtcgggtgg cctccccccg gatatcggct gctccgtcac accttcagga
     5221 tggttaccgc gcagttgttc ccggcaccca gaacgtgggc gagtccgacc ttggcattcg
     5281 gcacttgcct tttccccgcc tctccccgta attgggtcac gatttcaaag acctggccca
     5341 ggtgggatgc cccaatcggt tctcccttgc taatcaatcc tccgcttgtg ttcaccgggt
     5401 attttccgcc gatttcggtg gcgccttcat ccaggagacg cccaccctcg cccttcttgc
     5461 agaggcccaa ctgttcatag atttcaatct cgcagaaggc gtccgtgtcc tgaacctcga
     5521 aacaattgat ttcttggggg ccgatccccg cctgctggta agcttttttg gcggccactt
     5581 ccgtcgggcc agggttcatt gctttggcac tcaagctgtc cacgggggcc cgaaaatcag
     5641 cggaatagta ggcattcgtg ggaacgcagg cggcaacggt aatcggcttg ctggtaaatt
     5701 gggcagcctt ttctttcgga cagaggatca cggcagcaga gccttcgttg ggcgcacaga
     5761 tttccaggag atggatgggg tcgcagacca agggagagtt cataatttcc tcgatgccaa
     5821 attccttccg gtacatggcg taaggattat gaacgctgtt tttgtggttt ttcaaggcca
     5881 ccttggcgag gtgcaaatcg gtggtcccat attcatgcat gtgccgccgc gctcgcatgg
     5941 cccaataact cggattcgtg tccatgccca tttgaatttg ccatttttca tagatcatag
     6001 aagggtccat aaatccccgg ggcatttttt ctgtccccag gaccattacg atatcaaaag
     6061 ccccggcctg aattcctaaa atcgcctggc ttaaggcgat tcccccactc gcgcaggcag
     6121 cttcaatatc gcaaatgggg atccccgtag cccccatagg ctttaggatt ctggcccccg
     6181 aagtggcggg aagatacatc tttgaaaggt aggccatttg gacgtctttc cactgcaaat
     6241 tggcgtcccg cagagccatt tgggcggcct cttggcccaa ttccaggtag gacttgtttt
     6301 cgaacctgcc gaacttatga atcccgacac cgatgatggc aacctctttc atctgcatcc
     6361 ctcctaaacc ggcccaaacc ggaaggccat aacctcgttc aggttttcat ccgtatacag
     6421 gggatacacc tccatttcca attccattcc caccttcatc ctttccaggt cgcattgaat
     6481 cggcgagagg acccgaattc cctcgggcaa ctccaccagc cccaagccaa aaggaacgaa
     6541 agggtccggc cccttataat cgcccggcgg cttatggcga atgaccgtat aggtccataa
     6601 tttcccttgt cggcttagcg ggacctcggt caggtttcct cctccgcaat ttgagcaggt
     6661 ctgccttttc cccaaggaaa tttcactgca atcccggcat tggcttccca tgagtcttac
     6721 ccgttcaacg ggggataggg gagccgtaaa taaaccttcc ttgatcggga tttgtttttt
     6781 ctcgtttgag cttggctgtg gcatagatcg tcccctccta ttcgaaaaga atcctcgaat
     6841 atttttccaa aaaaagggca atttc
//
LOCUS       DASPRR010000194         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1013922, whole genome shotgun sequence.
ACCESSION   DASPRR010000194 DASPRR010000000
VERSION     DASPRR010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013922"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1378)
                     /locus_tag="VEL68_07575"
     CDS             complement(32..1378)
                     /locus_tag="VEL68_07575"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXZ35861.1"
                     /translation="MTESTSMKDHVFYRNSKKSYPTAVRGEGIYIYDSAGKRYIDGSA
                     GAAVVGIGHGVKEITQAMVRQAEKISFAHGSQFTSEASIELASKLIRLAPEGMTRVYF
                     LSGGSEAVETAVKLARQYQVERGKPEKFKVISRWTSYHGNTLGALALSGHSGRRRLYL
                     PLMLHTPHVAPPYCYRCPFGLQPETCSLECAADLERTILYEGPDSVSAFIAEPMVGAT
                     AAALIPRAGYYEKIREICDRHDVLFISDEVMTGMGRTGKNFGIDHWKVKPDMIVAAKG
                     LSSGYTPMGAVIVREEIHRAIQEGTGAFVHGHTYSQNPLSAAIGCAVVDYLLKQDLIN
                     RSAKMGEYFLEALKTLDKHQFVGDVRGRGLFAGIEFVKDKRTKEPFDPKLKLNGLIGS
                     GAFEKGLITYPGGGGVDGIRGDHILLAPPLIISKEQIDELVRILDQTFTEVLREIS"
     gene            complement(1465..3480)
                     /locus_tag="VEL68_07580"
     CDS             complement(1465..3480)
                     /locus_tag="VEL68_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009728641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HXZ35862.1"
                     /translation="MSSTQEAPGPGGNEREPVAPINVDDLMEKFDREARYLRITGLWA
                     TVVKIIAIGFSLFQLYTALFGVLEAQIQRPTHLMFALALIYLLYPTSGKSKIQVKVHP
                     FDLSLAILSCGLGLYSILNYETLMESAGIYSDVDFIMGILGIVLTLEATRRIVGLPIV
                     IIAVGFLLYAYLGPYFPGFLGHRGYGLKRMVTHMWFTTEGIMGIPLGVSATFIFLFIL
                     FGSFLTSTGIMDFFMDIANAVAGWASGGPAKVAVIASAFEGTVSGSSVANTVGSGSFT
                     IPMMKRLGYRPEFAGAVEAAASTGGQIMPPVMGAAAFLMAEFINMPYVEIAKAAAIPA
                     LLYFTGVWVVVHFEAKKYGLKGLPREQLPKLMKIAKERSYLILPLIGIIYFLVEGSTP
                     MRAALAGLGLCIAVGMVRKTTRLNLSKFLETLESGARGALGVAAACATAGIIVGVVTL
                     TGLGLKMGDGLIALAGGMLILTLAFTMITSLILGMGAPTTANYVITSTIAAPALIKLG
                     VPILAAHMFAFYFGIIADLTPPVALAAFAGAAIARANPFKTGVIATKLAIAAFLVPYI
                     FVYNPSLLLIKATPIEIAQMILTAIIGMIGLGAAVEGWYWTHMSWWERIASFASGLLL
                     IDPGTLTDIIGVCILGLITAIQYRKWRAARSLPRVKGASKESLAVDP"
     gene            complement(3563..4558)
                     /locus_tag="VEL68_07585"
     CDS             complement(3563..4558)
                     /locus_tag="VEL68_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ35863.1"
                     /translation="MERKPRMSGRKKMAVILLILGMALGANTGGWAQQTKLLFATGGI
                     AGTFYPLGGAIAQVWNQKIPGLNVTVQASGGSVENVRLLGSGNAELGLCMNDIAYYGQ
                     NGLEVFKAKNEKYTNFSAIGNIYPDVVQIFARKDSQIQSLTDFKDKKISVGPPGSGTE
                     ISARQVLGIYGLDFKTRKDFKAQYLSFAESADHFRDRLIDGGYFVVAVPNSALQDINL
                     MHPIRFLEIDDASFQKIVKAYPLYARFEIPSNSYQGQTAPVKTIAVYSAVVVRNELPA
                     DLVYQMTKTVFEERAVIAQGHAAGKYITPETFTAGIAIPFHPGAQKYLKEKGLLK"
     gene            complement(4613..>5350)
                     /locus_tag="VEL68_07590"
     CDS             complement(4613..>5350)
                     /locus_tag="VEL68_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HXZ35864.1"
                     /translation="GKVSLATVSRLSRALGFKGFPEFQRELQRLFRHRLTTTSRLQNT
                     VKKTAGEKNILAKVLQNDMVNIAETLKQTSPAEFQRFVKSLASAARIVIVGLRSAHSL
                     AVFMAVALEFLQKEVWLVQPGMGDMWDRLTGLKKGDVVVGISFPRYTKQTVEVLRFAK
                     AQGLKTLAITDTLLSPLAHYADHVLTARYKMDSFIESFTAAFSLINALVTALGIQSPQ
                     NTYASLQRLERIWRTQQIYYRDEEEKR"
BASE COUNT         1130 a         1539 c         1519 g         1162 t
ORIGIN      
        1 tcatgaaacc gtaaatactt ttttccccac atcaagaaat ctcccttaga acctcggtga
       61 aggtctgatc caggattctc accagctcgt cgatctgctc cttgctgatg ataagcggcg
      121 gggccaggag aatgtggtct ccccggattc catccacgcc acccccgcca gggtaggtga
      181 tcagcccttt ttcaaaagcc ccgctcccga tgaggccgtt gagctttagc ttggggtcga
      241 agggttcctt ggtcctttta tcctttacga attcgatccc ggcgaagagg cctcggccgc
      301 gcacatcccc tacaaattga tgcttgtcca gggttttcag agcctccagg aaatattccc
      361 ccatcttggc cgaccggttg atgaggtctt gcttcaacag atagtctacg acagcgcagc
      421 cgatggcggc ggaaagggga ttctggctgt aggtgtggcc gtggacaaag gctcccgttc
      481 cctcctggat ggcccgatga atctcctccc gcacgataac cgcccccatg ggcgtgtacc
      541 cgctgctcaa acctttggcg gccacgatca tgtccggttt taccttccag tggtcaattc
      601 caaaattctt tcccgtcctc cccatcccgg tcatcacttc gtcggagatg aaaagcacgt
      661 catggcggtc gcagatttcc cggattttct cgtagtagcc ggcccgggga atcagggcag
      721 ccgctgtggc cccgaccatg ggctcggcga tgaaggcgga gaccgaatcc ggtccctcgt
      781 agaggatcgt tctctccagg tccgccgcgc attccaggga acacgtttcg ggctggagcc
      841 cgaaggggca gcggtaacaa tagggcgggg ctacgtgggg ggtgtgaagc atgaggggca
      901 aatagagccg ccttcttcct gagtgcccgg aaagagccaa ggctcccagg gtatttccgt
      961 ggtaactggt ccagcgggaa atcactttga acttctccgg cttcccccgc tccacctggt
     1021 attgccgggc cagtttcacg gctgtttcaa cggcttccga tccgccggac aagaagtaaa
     1081 cacgggtcat cccctcgggg gccaggcgga tcagcttgga ggccaattcg atcgaggctt
     1141 cgctggtgaa ctgggagccg tgggcgaagg agatcttttc cgcttggcgg accatggctt
     1201 gggtaatctc tttcactcca tggccaatcc ctacgactgc ggccccggct gagccatcga
     1261 tgtacctctt tcccgcagaa tcataaatgt aaatcccctc accgcggact gcggtggggt
     1321 atgatttttt ggagttgcgg taaaaaacgt ggtccttcat gctggttgac tctgtcattt
     1381 cattttcctc ccaagtcgtt cgtcattaag acccaaaccc gcaatctttt cggcccgtca
     1441 tttggcctcc cggatgggtt tcccctaagg atcgactgcc aggctttctt tggacgctcc
     1501 cttcacccgg gggagagagc gggccgctct ccacttccgg tactggatcg cggtgatcag
     1561 gcccaagata cataccccga taatatcggt aagcgtgccg ggatcgatca aaagcagccc
     1621 gctggcgaaa gaagcgatcc gttcccacca gctcatgtga gtccagtacc acccctccac
     1681 tgcggcccca aggccgatca tcccgatgat cgcggtcaga atcatctggg cgatctcgat
     1741 aggggtggct ttgatcagga ggagggatgg gttgtacaca aagatatagg ggaccagaaa
     1801 agcggcgatg gccagcttcg tggcaatgac cccggtcttg aagggattgg ccctggctat
     1861 ggccgcgccg gcaaaagccg caagagccac ggggggagtg aggtccgcga tgatcccgaa
     1921 atagaaagcg aacatgtggg ccgccagaat ggggactccc agcttaatga gcgccggagc
     1981 cgcaatggtt gaggtgatca cataattggc cgtagtggga gcacccatcc ccaggatgag
     2041 ggaagtaatc atggtaaaag ccaacgtcag gatcaacatt cctccggcca gggcaatgag
     2101 tccgtctccc atcttgaggc ccaagccggt gagcgtcact acgcctacga tgatgcctgc
     2161 cgtagcgcag gccgcagcca cccccagagc tccacgggcc cccgactcca gggtttcaag
     2221 aaatttggaa aggttcaggc gggtcgtctt ccgcaccatg cccacggcaa tgcaaagccc
     2281 gaggccggcg agcgctgctc tcatcggcgt cgacccttca accaggaaat agatgatccc
     2341 gatcaaagga agaatcaaat agctgcgctc tttggcgatt ttcataagct tcggaagctg
     2401 ttcccggggg agccctttca acccgtactt cttggcctcg aagtggacca ccacccagac
     2461 cccggtaaaa tagaggaggg ctggaatggc cgctgccttg gcgatctcca catagggcat
     2521 gttgatgaac tccgccatga ggaaggctgc ggcccccatc accggaggca tgatttggcc
     2581 gccggtggag gctgctgctt ccacggcccc ggcaaattcc ggcctatatc ccaaccgctt
     2641 catcatgggg atagtgaaac ttccggaccc gacggtattg gccaccgagc ttcccgagac
     2701 tgtaccttca aaagcactgg cgatcacggc gaccttagcg ggtccgcccg aggcccaacc
     2761 ggccacggca ttggcgatat ccatgaagaa atccatgatt ccggtagagg tgagaaagga
     2821 cccgaaaagg atgaacagga aaatgaaagt ggccgagact cccaggggaa tgcccatgat
     2881 cccctcggtg gtgaaccaca tgtgggtgac catccgcttc agcccgtagc cccggtggcc
     2941 caggaacccc gggaaataag ggcccagata ggcgtagagc agaaaaccga cggcgatgat
     3001 cacaatcgga aggccgacaa tcctgcgggt ggcttccagg gtcaggacga tgccgaggat
     3061 tcccatgatg aaatcaacat ccgaataaat tccggcgctc tccatcaagg tctcatagtt
     3121 caagatcgag tacagcccca ggccgcagct gaggatggcc aggctcaggt cgaaggggtg
     3181 aaccttcacc tggattttgc tctttccgct ggtggggtag aggagataga tgagcgccag
     3241 ggcaaacatg aggtgggtgg gacgctggat ctgggcttcc agcacgccga agagggcggt
     3301 gtaaagctgg aacagtgaaa atcctatggc gatgatcttc acgaccgtgg cccatagacc
     3361 ggtgattcgc aggtaccgcg cctcgcggtc gaatttttcc atcaagtcat ccacgttgat
     3421 gggcgctacg ggctctcttt cattgccgcc agggccgggg gcctcttggg ttgaagacat
     3481 gcttcctcct cctcattcgg gagctctcga ctccttactc ccgggaaccg gcagaggagt
     3541 cgagagggtc agatcggtgc ggctatttca gaaggccctt ctccttcaga tatttttgcg
     3601 cccccggatg gaaggggatg gcgatccctg cggtaaaggt ttccggggtt atgtattttc
     3661 ccgccgcgtg tccctgggcg atgaccgctc tctcttcaaa gaccgtcttg gtcatctgat
     3721 aaacgaggtc ggcgggcagt tcattccgga ccacgacagc ggaatagacg gcgatggtct
     3781 tcactggagc ggtctgcccc tggtaggaat tggagggaat ttcaaaccgc gcgtacaggg
     3841 gataggcctt gacgatcttc tgaaaagagg cgtcgtcgat ctccaggaat cgaatgggat
     3901 gcatgaggtt gatgtcctgc aaggccgaat tggggaccgc caccacgaaa taaccgccgt
     3961 cgatcagtct gtcgcggaag tggtcggcgg attccgcgaa ggaaaggtac tgagctttga
     4021 aatcctttct ggtcttgaag tccaggccgt agattcccag gacctgccgg gcgctgattt
     4081 ccgttccgct ccccggagga cccacggata tcttcttatc cttgaaatcg gtaagagact
     4141 gaatctggct gtctttccgg gcaaagatct ggaccacatc cggatagatg ttgccaatgg
     4201 cggaaaagtt ggtgtatttt tcgtttttcg ccttaaagac ttccagccca ttctggccat
     4261 agtaagcgat gtcgttcatg cagagcccca gctctgcgtt cccgctgccc agcaacctta
     4321 cgttttccac ggacccgccg ctcgcctgca cggtcacgtt caggccgggg atcttctggt
     4381 tccagacttg ggcaatggct cccccgaggg gatagaaagt tcccgcgatt cccccggtgg
     4441 cgaagagcag cttggtctgc tgcgcccagc cgcccgtatt cgctcccagg gccatcccca
     4501 gaatcagcag gatgaccgcc atcttcttcc ggccgctcat tcgaggtttc ctttccatga
     4561 ccttacctcc ttccttcttc tttttgatgc cttggtatga aggctgtatc ccttaacgct
     4621 tctcctcctc gtcccggtag taaatctgct gtgttctcca aatcctttcc aggcgctgca
     4681 acgaggcgta ggtgttttgg ggggactgga ttccgagcgc cgtgaccagg gcgttgatga
     4741 ggctgaaggc tgcggtaaaa gattcgatga aggaatccat tttgtaacgg gcggtcaaaa
     4801 catgatccgc ataatgggcc aggggagaaa gcagagtatc cgtaatcgcc agggtcttca
     4861 atccttgggc cttggcaaac cgcaggactt cgaccgtctg tttcgtatac cggggaaagc
     4921 tgatccccac aactacatcc ccctttttca ggcccgtcag acgatcccac atatctccca
     4981 tcccgggctg gacgagccac acttcttttt ggaggaattc gagagccacg gccataaaaa
     5041 cggctaggga atgggcactg cgcaacccga caatcacgat tcgagcagcc gatgccagac
     5101 tcttcacaaa cctctggaac tcggccggag aggtttgttt cagcgtctcg gctatgttaa
     5161 ccatgtcgtt ctgcagaacc ttggctaaaa tattcttctc cccggctgtc ttcttcaccg
     5221 tattctggag gcgggaagtg gtggtcagtc ggtggcgaaa aaggcgctgc aactcccttt
     5281 ggaattccgg gaaccccttg aatcccaatg ctctggatag ccgggaaacg gtggctaggc
     5341 tcacctttcc
//
LOCUS       DASPRR010000195         4912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1984134, whole genome shotgun sequence.
ACCESSION   DASPRR010000195 DASPRR010000000
VERSION     DASPRR010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984134"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VEL68_07595"
     CDS             <1..1228
                     /locus_tag="VEL68_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXZ35865.1"
                     /translation="AEKFSALAASHLGIKKDLVATPLLHDTPGEIAQPCGRVRDWKKG
                     EVEPIPGQTLPSLAVLTRDYSNVYKMMTALGPLAAKSEIGAKGVSWSAAEEYEELKER
                     LGTVAEEGLTRGMPTLAEGRQVAEAILGLAPETNGKAAQKAWKDLEKKTGLNLAHLSG
                     DRLGERFTFADLEAMPRKIITSPIWSGLESGGRKYSPFVINVEEKVPFRTLTGRAQFY
                     LDHEWMLAFGEGLPVFRPPLSLDDLGSTRVRREAGRKEIILNYLTPHSKWSIHSTYSD
                     TLIMLTLFRGGEAIWINHEDAESIGVRDNEWLECFNVNGVVMAKAVVSHRVPRGKAFM
                     YHAQERLISTPGSRISGQRGGTHNSVTRILLKPTQMIGGYAQLSYAFNYYGPTGAQRD
                     EVIVVRKAEGVDWYED"
     gene            1218..2645
                     /gene="narH"
                     /locus_tag="VEL68_07600"
     CDS             1218..2645
                     /gene="narH"
                     /locus_tag="VEL68_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HXZ35866.1"
                     /translation="MRIKAQMAMVMNLDKCIGCHTCSIPCKNVWTNREGAEYIWYNNV
                     ETKPGIGYPKRWESQEDHRGGWVVKGGGLSLRAGGRLEKLLKVFHNPDLPQIDQYYEP
                     WTYDYAKLIESPIRKHQPATRPRSLITGKEIPLQWGPNWEDDLAGVCFTGKQDVNFSG
                     LEAESYLKFQSAFMFYLPRICEHCLNPSCVASCPSGALYKRDEDGIVLVDQKRCRGWR
                     YCVSGCPYKKVYFNWKAGRSEKCLFCYPRVENGMPTLCAESCVGRIRYVGVLLYDADR
                     IKEVASTQEERGVYSAHASIFLNPHDPEILREAIAGGIPGSYLDAARLSPVYKLAVEW
                     KLAFPPHPEFRTLPMVWYIPPLSPILPGPGDEERGEPDPGRMRIPLKYLANLFSSGDE
                     KPVKAALEKLMALRNYWRSVLVDKTPDTRVLEKAALTPEIAREMVYLLGVGKYSERYR
                     IPTVKAEEAGNPGALQGRKGFNFEE"
     gene            2647..3210
                     /gene="narJ"
                     /locus_tag="VEL68_07605"
     CDS             2647..3210
                     /gene="narJ"
                     /locus_tag="VEL68_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HXZ35867.1"
                     /translation="MEEERQVLFRIGSLLLHYPDEELFRSLGSLGEILGDLPPHGAAG
                     KFREFVDYLGKTPLLRAQEEYTRTFDFTPLTCLSLTYHRWGEGKERGEALLDLKHTYF
                     KEGYEMGEGELPDYLPLVMEFMAVGGWEETRRIMKEYTNSVVALAARLHELGSPYADL
                     LEGVERLFGEDGIPGARDDGNVEPSGL"
     gene            3179..3877
                     /gene="narI"
                     /locus_tag="VEL68_07610"
     CDS             3179..3877
                     /gene="narI"
                     /locus_tag="VEL68_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HXZ35868.1"
                     /translation="MMETWNLLAFEVIPYLCLTTFAVGHLYRYFTDPFRWNAHSSEIL
                     EKGSLKYASHLFHWGVILTFFGHAGGLLIPQSIFDAAGIDGSAHTRISIYLGAPFGLA
                     AFLGILFLTWRRLAKERVWMVTSVKDWVTLLLLLEVIGMGTYNVLFGGYYILDTVAPW
                     IRSIVVLSPNPALMKDVPPTYQAHILGAFILFAFSPFSRLVHIWSVPLGYLFRRYLLH
                     RQWDAEKGLNVFEK"
     gene            3861..>4912
                     /locus_tag="VEL68_07615"
     CDS             3861..>4912
                     /locus_tag="VEL68_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HXZ35869.1"
                     /translation="MFSKSRLSSAVAGFLPAYFSLVMATGIVSIASHLQGFDFFAFPL
                     FWLNLLFYGILWAITGARIIYYPTRFLEDVKDHVRGVGCFTIVAGTCVLGSQFVILLQ
                     SFEWGVFFLGVGTFLWVVLIYGIFTTLAISPHKPSLEKGIHGGWLVAVVATQSISVLA
                     GLTDPHFDHNREAMLFFSLAMFLMGGILYLLIIFLILYRFLFFVLKPEDLKPQYWVNM
                     GAAAISALAGAVLALNGQSAQFLAQLKPFVLGLALFFWASATWWIPYLFLLGVWRYLV
                     RRVKFSYEPHYWSLVFPLGMYTTCTTQLAKAVHLDFLMEIPRYFIYAAILAWGLTFWG
                     LLRTFARFLFSAEPAAG"
BASE COUNT          988 a         1379 c         1447 g         1098 t
ORIGIN      
        1 cgccgaaaag ttctccgccc tggcggcttc ccatctgggc atcaagaagg acctggtggc
       61 cacccctctc ctgcacgata cccccgggga gatcgcccag ccctgcggca gggtaaggga
      121 ttggaagaag ggggaggttg aacccatacc cgggcagacc cttcccagcc tggcggttct
      181 cacccgggac tattccaacg tatataagat gatgaccgcc ctgggtccgc tggcggccaa
      241 atcggagatc ggcgccaagg gggtatcctg gagcgcggcc gaagaatacg aggaattgaa
      301 ggaaaggctg ggaacggtgg ccgaagaagg tctgacccga ggaatgccca ccctggccga
      361 gggaaggcaa gtggcggaag cgattttggg gcttgccccg gagaccaacg ggaaggcggc
      421 gcagaaggcc tggaaggact tggagaagaa aacgggtcta aacctggccc atctgagcgg
      481 ggaccgcctg ggcgaacggt ttacctttgc ggacctggag gccatgcctc ggaagatcat
      541 caccagcccg atatggagcg gcctagagtc gggaggcagg aaatattccc ccttcgtgat
      601 caatgtggaa gagaaggttc ctttccgcac tcttacgggg cgcgcccagt tctacctgga
      661 ccatgaatgg atgctggcgt tcggcgaggg gcttcccgtc ttccggcctc ccttgagcct
      721 ggatgacctg ggctccactc gggtgaggcg ggaggcgggt cggaaggaga tcatcctgaa
      781 ctacctcacc cctcactcca agtggtcaat ccacagcacc tactcggaca ccctcatcat
      841 gctgaccctg ttccggggag gggaagccat ctggattaac cacgaggatg ctgagtccat
      901 cggagtcagg gacaacgagt ggctggagtg cttcaacgta aacggggtgg tcatggccaa
      961 ggcggtggtg agccaccggg ttccgagggg gaaggccttc atgtaccatg cccaggaaag
     1021 gctgatcagc acacccgggt ctcggatttc cggccagcgg ggcggcactc acaacagcgt
     1081 gactcgcatc ctactcaagc cgactcagat gatcggtggg tacgcccagc tcagttacgc
     1141 cttcaactat tacgggccca cgggagcgca acgggacgaa gtgatcgtgg tgcgcaaggc
     1201 ggagggggta gactggtatg aggattaaag cccagatggc catggtcatg aacctggata
     1261 agtgcatcgg ctgccacacc tgcagtattc cgtgcaagaa cgtctggacc aaccgggagg
     1321 gggccgagta catatggtac aacaacgtgg agaccaagcc ggggatcggt taccccaagc
     1381 ggtgggaaag ccaggaggat caccggggcg gctgggtggt gaagggtgga ggtctttcgc
     1441 tgcgggcggg ggggcggctg gagaagctgc tgaaggtttt ccataatcct gatctcccgc
     1501 agatcgacca atactatgaa ccctggacct atgactatgc caaactgatc gaaagcccga
     1561 tacgaaaaca tcaacctgcc acccgcccga ggtccctgat caccggcaaa gagatccccc
     1621 ttcagtgggg acccaactgg gaagatgacc tggccggggt ttgtttcacc ggaaagcagg
     1681 acgtaaactt ctccggtctg gaagccgagt cctacctgaa attccagagc gccttcatgt
     1741 tttacctgcc ccgcatctgc gagcactgcc tgaacccatc ctgcgtggcc tcttgccctt
     1801 ctggggcgct ttacaaacgg gacgaagacg gaatcgttct ggtggatcag aaaaggtgtc
     1861 ggggttggcg gtactgtgtc tcgggatgcc cctacaagaa agtgtatttc aactggaagg
     1921 cgggacggtc ggaaaagtgc ctcttctgct acccccgggt ggagaatggg atgcccactc
     1981 tctgcgccga gtcttgtgtg gggcggattc gctatgtcgg agtccttcta tacgatgccg
     2041 accggatcaa agaggtcgct tcgacccagg aagagcgggg agtctattcc gcacacgctt
     2101 cgatcttctt aaacccccat gacccggaga tcctaaggga agctatcgcc ggggggattc
     2161 ccggcagcta cctcgatgct gcccggttgt ctccggtcta taagcttgcg gtggaatgga
     2221 agctggcctt tccaccgcat cccgaatttc gcaccctccc catggtgtgg tacataccgc
     2281 cgttgagccc catcttacca gggcctggag acgaagagcg cggggaaccg gaccccgggc
     2341 ggatgagaat ccccttgaaa tacctggcca accttttctc ctccggggat gaaaaaccgg
     2401 taaaggcggc tctcgagaag ctcatggccc ttcgcaatta ctggcgctcg gttttggtgg
     2461 acaaaacccc agacacgaga gttctggaaa aagccgctct cactccggag atagcacggg
     2521 agatggtcta cctcctgggg gtgggcaaat attccgagcg ctaccgtatc cccacggtga
     2581 aggcggaaga ggcggggaac cccggggcct tgcagggcag gaaaggtttc aacttcgagg
     2641 agtgagatgg aagaggagcg gcaggtccta ttccgaatcg gttcccttct tctgcattac
     2701 ccggatgaag aactcttccg ttccctgggc tccctggggg agattctcgg agacctccct
     2761 ccccatgggg cggctggcaa attccgggaa ttcgtggatt acctcggaaa aacgcctctc
     2821 ctgcgggctc aagaggaata tacccggact ttcgatttta cccctctaac ctgtttgagc
     2881 ctcacttatc accggtgggg agaggggaag gaaagggggg aagccctcct cgacctgaag
     2941 cacacctact tcaaggaggg ctatgaaatg ggggaggggg aacttccgga ttacctcccc
     3001 ctggtcatgg aatttatggc cgtgggtggc tgggaagaaa caagaagaat aatgaaggaa
     3061 tacaccaatt cggtggttgc tttggcggca cgcttgcatg agttggggtc tccttatgcg
     3121 gacctgctgg aaggggtcga gcgacttttt ggcgaagatg gcatcccagg agcaagagat
     3181 gatggaaacg tggaaccttc tggcctttga ggttattcct tacttgtgtt tgaccacctt
     3241 cgcggtggga cacctctacc gctacttcac cgaccccttc cggtggaacg cccactccag
     3301 cgaaatcctg gaaaagggaa gcttgaaata cgcttcccac ctttttcatt ggggagtcat
     3361 tctcaccttc tttggccatg cgggaggcct cctcatccct cagagcatct tcgacgcagc
     3421 ggggattgac ggatcggccc atacccgaat ctccatttat ttgggggcgc cctttggtct
     3481 ggcggccttc ttagggatcc tgttcctcac gtggcgccgg ctggcaaagg aaagggtctg
     3541 gatggttacg tccgtgaagg attgggtcac cctgttactc ctccttgagg tcatcgggat
     3601 gggaacctat aatgtgctct ttgggggcta ctacatccta gatacagtgg ccccctggat
     3661 acggtccatc gtggttcttt ccccgaaccc ggcccttatg aaggatgtgc ctccgaccta
     3721 tcaggcccac atcctggggg cctttatcct tttcgccttt tcccccttca gccgcctggt
     3781 tcatatctgg tcggtccctc tgggctacct tttccgccgc tatctcctcc atcgccaatg
     3841 ggacgccgag aaggggttga atgttttcga aaagtagact ctccagcgcc gtcgccggtt
     3901 ttctccccgc ctatttttcc ttggtcatgg ccacaggcat cgtttccatc gcttcgcatc
     3961 tgcaggggtt tgatttcttc gcctttcccc ttttttggct gaacctgctc ttttacggga
     4021 ttctttgggc gatcaccgga gcccgaatca tttactatcc tacgaggttc ttggaagatg
     4081 taaaggatca cgtgcgggga gtcgggtgct ttaccatcgt ggctggaacc tgtgtcctgg
     4141 gaagtcaatt cgtgatcctt ctccagtctt ttgagtgggg ggtatttttt ctgggagtag
     4201 gcacgttcct atgggttgtg ctgatctacg ggatattcac aactctcgcg atcagtcccc
     4261 acaaaccttc cctggagaag ggaatccacg gaggatggct ggtggccgta gtggccaccc
     4321 agtccatatc cgttttggcc ggcctgacgg atccccattt cgatcataac cgggaagcaa
     4381 tgctcttctt ttccctcgcc atgtttctta tggggggaat tctctacctg ttgattatct
     4441 tcctgatcct ctaccgattt ctgttctttg tcctgaagcc cgaagacctg aagccccagt
     4501 actgggtaaa catgggagcg gcggccattt ccgctcttgc gggggccgtc ctggcactga
     4561 acggtcaatc ggcccaattc ctcgcccagc tcaagccctt cgtccttggc ttggccctat
     4621 ttttctgggc gagtgccacc tggtggatac cttatctctt cctcttgggg gtctggcgat
     4681 acttggttcg tcgggtgaaa ttctcctacg aaccccatta ctggagtctg gtcttccctc
     4741 tggggatgta cactacctgc actactcagc tggccaaggc cgtccacttg gattttctaa
     4801 tggagatccc gcggtatttc atatatgcag ccattctggc ctggggacta accttctggg
     4861 gcctcctgag gaccttcgcc cgcttcctct tctctgcgga accagccgct gg
//
LOCUS       DASPRR010000196         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1823164, whole genome shotgun sequence.
ACCESSION   DASPRR010000196 DASPRR010000000
VERSION     DASPRR010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823164"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VEL68_07620"
     CDS             complement(<1..679)
                     /locus_tag="VEL68_07620"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXZ35870.1"
                     /translation="METNSFKRALDAKEFVYTLELVPGRGARGKVIEDTMRMAEKAVQ
                     GGKIHALSITDNAGGHPALFPNVLGREICNLGMNPLIHFTCKDKNRNQIESILYALDR
                     IGIENLLVMTGDYPLYGFEGKAKPVYDLDSVQLLHLIKNMNEGKCIDGKAPGGGAECP
                     PTRFLKGCAVSPFKMQEAEVLAQYFKLYKKAKAGADFVITQVGYDARKFDELLRFMQQ
                     QGIQVPII"
     gene            1311..2537
                     /locus_tag="VEL68_07625"
     CDS             1311..2537
                     /locus_tag="VEL68_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35871.1"
                     /translation="MKKFYWVLMIMICAFILSTAGSSWGQPKTLKIGAPIPLGGVGGA
                     YVGLGWGYNDCQAYLTEKGGIKGNKVIYLVEDNRWDVTTEIAIINRQLSKEPKEELVL
                     FCGNLITGTLKAMSEKINKEVRIPSIALSFSVEMFGKQGGPSKYPYYFTTGPDYSQQI
                     GLLLRYIKKDHKKDAAAKLAVVYSPTEYGRDPLADMKEDAAKLGIKIVAEEEVAVTAS
                     DVSTQIINIRKAQPDYLIWHGFLPGMLVAPVFLKTARQYLPKIPILGTDFQTSPTMIA
                     AGGPAADGIIGVNTRVQWQETDNPFVKLIQEQAAKRERKLPWAEQSNYILGWTMAILG
                     AHALDAAAETGDFSREGVKKAMENSVWDFYGMYEGKKFSYQSHKNPMARIYKASGAKK
                     EWDAITPWLNVDEELK"
     gene            2559..3443
                     /locus_tag="VEL68_07630"
     CDS             2559..3443
                     /locus_tag="VEL68_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35872.1"
                     /translation="MGFFLQSFFSGMLSGLSYGLLGLGLVLIFRASEAFNFAVGEFMV
                     VGGFLFYLLHFNWHLPFLVALPAGLLAAAVFGAIVERTTIKSLMGRPALSMTIITLGL
                     SNILAGGLQATAGSNPYPFSLDLPDLTIEVGTLIYPSPQVWAGVLSLVFFGLALYFIY
                     RTRWGLVIQATSESQPKALCYGINAQFVLLLVWAVAAVCIAVSGIVVANLGALSVQTS
                     LVGLRAIPVVLIGGLDSIPGALVGGLIVGVVEALTGAYVEPLGLIGFKEVVPYLVLLV
                     TLFIRPYGLFGTVRIERV"
     gene            3445..4230
                     /locus_tag="VEL68_07635"
     CDS             3445..4230
                     /locus_tag="VEL68_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35873.1"
                     /translation="MLRVENLRVVYQEVISVLHGVSLKVPDGEVTVLLGANGAGKTTM
                     LRTICGLLPVYDGKIIEGSVLLDDAPLHRLASSTIVKRYRTTYVMEDRPVFWYLTAEQ
                     NLKAAAFSRWDRQVPADVEKVMTVFPKLASLRNTRAGYLSGGEQQMLAIGMALMTRPK
                     LLMLDEPSLGLAPLIVRELFSIIRNLNREGLTIFLVEQNAHAALSVGHTGYVIENGRI
                     VLDGPSGELIKNKDVREFYLGTARIDEKRFLEAKRYKRRKRWL"
     gene            4221..5006
                     /locus_tag="VEL68_07640"
     CDS             4221..5006
                     /locus_tag="VEL68_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35874.1"
                     /translation="MALDPVLLEISDLTLSFGGVMALRDLSLRVPRGVFASIIGPNGA
                     GKTSLLNCINGHYRPQRGRVSLENRDITHSRPHQIARMGVSRTFQKIELFSHMSVLDN
                     VKLGRHFLMKASPVSCFFRLPGMVQEEFRHRRELDDEVIDFLGLSAFRNHIVGGLPHG
                     IRKRVDLARALAMKPKLLLLDEIMAGMSMEEKEDIASYLMDIHRDWGTTMIWIEHDLA
                     AVMELSTWVTVLNFGEKIAEGTPVEVQKNPAVIEAYLGTGTEI"
BASE COUNT         1092 a         1499 c         1500 g         1182 t
ORIGIN      
        1 cgatgatggg aacctggatg ccctgctgct gcataaagcg gagcaactcg tcgaacttgc
       61 gagcatcgta tcccacctgg gtgatgacga aatccgcccc cgctttggcc ttcttataca
      121 gcttgaaata ttgggccagg acctctgctt cctgcatctt gaagggggat acagcacatc
      181 ctttcaggaa gcgcgtcgga ggacattctg cccctccccc cggggctttc ccgtcgatgc
      241 acttgccttc gttcatgttt tttatcaagt gaagaagctg caccgaatcg aggtcataaa
      301 ccggcttggc cttcccctcg aaaccataga gagggtaatc cccggtcatt accaggaggt
      361 tctcgatgcc gatccggtcc agcgcgtaaa gaatcgattc aatctgattg cggttcttgt
      421 ccttgcaggt gaagtgaatg agggggttca tccccaggtt acagatctcc cgtccgagga
      481 cgttgggaaa gagggcgggg tgacctccgg cattgtcggt gatggaaagg gcgtggattt
      541 ttcccccttg aacggccttc tcggccatcc tcatggtatc ctcgattacc ttcccccgag
      601 cgccccttcc gggtaccagc tccagggtgt aaacaaattc tttagcatcc agggcccttt
      661 taaaactatt tgtttccatg cttcccttca tcccaccgcc cacaacgcgc ctatactttt
      721 ccctcgacta cgcggcagca aagtggatcc tatgaccgac atccgtaagg gcggtttgcg
      781 aaccgcccct acacctggct cgagaccggg aaatttcctc tttcctaaca tgtgcgcagt
      841 aattgctcag caacccctat aatctcaggg taggagagga tcccgtgaat gtcaagaaaa
      901 tgggttcgag cgaatttcga atgaatttct ccttgacttg ccgattcgtt tgttgctaat
      961 atccgtgccg taaatccata ttcctttctc ttgaatagcg ctttctcctt ttcggcaaag
     1021 ggagaaccgg tcatgctgaa tgaggattac taagagcccc gtcttcaaat ggagatgggg
     1081 ctttttgttc cgacccttcc ctgagcggcg tcaatctgag ccaatgaatc aaagattctt
     1141 ttctccagaa gtttaagtaa ttgggattca catctgctct tttcttcggc ctttacgaac
     1201 ttcctgaacg taagtagcac agagcataaa tagctttcag actacgagtt tcgggtttca
     1261 atttgggttt ttttggcagc ggcgtggccg cacggaaaaa ggagggtgaa atgaaaaagt
     1321 tttattgggt tttgatgatt atgatctgcg ccttcatcct gagcactgcc ggttcgagct
     1381 ggggtcagcc caaaacgctt aagatcggcg cccccatccc cctcggcgga gtgggaggag
     1441 cctacgtggg cctgggctgg gggtacaacg actgccaggc ttatctgacg gaaaaggggg
     1501 gcatcaaagg gaataaagtc atctacctgg tggaagacaa ccgctgggat gtaaccaccg
     1561 agattgccat tatcaatcgc cagctcagca aagaaccgaa agaggaactg gttcttttct
     1621 gcggcaacct gatcaccggg accctcaagg cgatgagcga gaagatcaac aaggaagtgc
     1681 gaatcccctc cattgccctc tccttttccg tggagatgtt tggcaaacag ggcggtcctt
     1741 ccaaatatcc ctattacttc accaccggcc ccgactattc ccagcagatc ggccttctcc
     1801 tgcggtatat caagaaggat cacaagaagg atgccgctgc caagctggcc gtggtctatt
     1861 ccccgacaga atacggccgg gaccccctgg ctgacatgaa ggaggatgcg gccaagctgg
     1921 gaatcaagat cgtggcggaa gaagaagtgg cggtcacggc ctcggatgtt tccacccaga
     1981 ttatcaacat ccggaaagcc cagccggatt acctgatctg gcacgggttc ctccctggaa
     2041 tgctggtggc cccggttttc ttaaagaccg cccggcagta tttacccaaa attcccatct
     2101 tgggtacgga tttccagact tccccgacca tgattgccgc gggcgggccc gctgcggacg
     2161 ggatcatcgg agtcaacacc cgcgtacaat ggcaggagac cgacaacccc ttcgtcaagc
     2221 tgatccagga acaggcagcg aagagagaaa ggaagctccc ctgggccgag cagtctaatt
     2281 acatcctggg gtggaccatg gcgatcctcg gggcccatgc ccttgacgcg gcggccgaga
     2341 ccggggactt ctcccgggaa ggggtgaaga aagccatgga aaattccgtc tgggacttct
     2401 acgggatgta tgaggggaaa aaattctcct accagtccca caaaaatccc atggccagaa
     2461 tctacaaagc ttctggcgcc aagaaggagt gggacgccat cactccatgg ctcaatgtgg
     2521 atgaggagct gaaatagctg gaggaccccc tcactaccat gggctttttt ctccaatcct
     2581 ttttctccgg gatgctgagc ggactctcct acgggctttt gggcctaggg ctggtgctga
     2641 tcttccgggc atcagaggcc tttaactttg ccgtaggtga gtttatggtc gtgggtggtt
     2701 ttctctttta tcttctccat ttcaactggc atttgccctt tctggttgcg ctgccggccg
     2761 ggctcctggc ggctgcggtt ttcggggcca tcgtggagag gacaaccatc aaatccctca
     2821 tgggaaggcc cgccctgtcc atgaccatca ttaccctggg gctttccaac atcctggcgg
     2881 gagggctcca ggccacggcg gggagcaatc cctacccctt cagcctggac ctcccggacc
     2941 tgaccatcga agtgggaacc ctcatctatc cttcgcccca ggtatgggcc ggcgttctgt
     3001 ccctggtgtt cttcggcctg gccctttatt tcatctaccg gacgcgctgg ggtctggtca
     3061 tccaggccac ttcggagagc cagcccaagg ccctctgtta cggaatcaat gcccagtttg
     3121 tccttctcct ggtctgggcc gttgccgccg tctgtatcgc cgtgtcggga atcgtggtgg
     3181 ccaatctcgg cgcgctttcc gtccagactt ccctcgtagg gttgcgggcg atcccggtgg
     3241 tgctgatcgg agggctggac agcatccccg gggccctggt gggggggctg atcgtaggag
     3301 tggtcgaagc gctgaccggg gcctacgtag agcccctcgg gctcattggt ttcaaggagg
     3361 tcgtccctta cctggttctg ctggttaccc tgttcatccg cccctatggc ctgttcggga
     3421 ccgtacggat cgagagggtg tgacatgctc cgggtggaaa atctgcgggt ggtttatcag
     3481 gaagtcatct ccgtcctcca cggggtctcg ctcaaggttc ccgacggcga agtaacggtc
     3541 ctgctcggag ccaacggggc ggggaagacc accatgctcc ggaccatctg cggcctcctt
     3601 ccggtctatg acgggaagat catcgaaggg agtgtgcttc tcgatgatgc tcccctccat
     3661 cggctcgcct cctctacgat cgtcaagcgg taccggacca cgtacgtgat ggaggaccgg
     3721 ccagtattct ggtatctcac cgcggagcaa aacctgaagg cggcggcctt ctcccgctgg
     3781 gaccggcaag tcccggcgga tgtggaaaag gtgatgaccg ttttccccaa gttggccagc
     3841 ctgaggaaca cccgggcagg gtacctctcc ggcggtgagc agcaaatgct ggccatcggc
     3901 atggccctca tgacccggcc caagctcctg atgctggatg agccgtcttt aggcctggcc
     3961 ccgctcatcg tgcgggagct cttcagcatc atccgcaacc ttaaccggga gggcctaacc
     4021 attttcctgg tggagcaaaa cgcgcacgcc gccctatctg tggggcatac gggctatgtg
     4081 atcgaaaacg gccggattgt cctggacgga ccttcgggcg aattgatcaa gaacaaagac
     4141 gtgcgggagt tttacctggg cacggcccgg atcgacgaga aaagattcct cgaagccaaa
     4201 cgctacaaac ggaggaagag atggctctag acccggtcct cctggagatt tcggacctga
     4261 ctctatcctt cggcggggtg atggccctta gagatctctc ccttcgcgtt ccaaggggcg
     4321 tctttgcctc cattatcggg ccgaacgggg cgggaaagac cagcctgctc aactgcatca
     4381 acggccatta ccgcccgcag agggggcgcg tttccctgga gaatcgggac atcactcatt
     4441 cccgcccgca tcagattgcc aggatgggcg tgtcccggac ctttcagaag atcgagctct
     4501 tctctcacat gagcgtcctg gacaatgtga aactgggaag gcatttcctg atgaaggcct
     4561 ctccggtgag ctgctttttc cggctgccgg gcatggtgca ggaggaattc cggcaccgcc
     4621 gggagctgga cgacgaagtg atcgacttcc tcggcctttc ggcgttccgc aaccatattg
     4681 tgggcgggct tccccatggg atccggaagc gggtggacct ggcccgggcc ctggccatga
     4741 aaccgaagct gctcctgctc gacgagatca tggccgggat gagcatggaa gaaaaagagg
     4801 atattgccag ctacctgatg gacatccacc gggactgggg aacgaccatg atctggatcg
     4861 aacacgatct ggccgcggtc atggagcttt ccacctgggt cacggtgctc aatttcgggg
     4921 agaagatcgc cgagggaacc ccggtcgagg tgcagaaaaa cccggcggtg atcgaagcgt
     4981 acctgggaac cgggacggaa atataagcat ttccagttgt atgtttccag ttgccggtaa
     5041 aggatcaaac cccgcaacgc gcaaaccgga acgattagac ttttggtcat ttgcgctatg
     5101 cgctttgccc tacgcgtttt tagggaaagg aaatggtgac cgataccctc atccagctgt
     5161 tcatttcccg ggcgaagcgc catcctgacc ggatagccct gcgggagagg cagtatggga
     5221 tctggattcc caccacctgg gcggaactct ggcagaaatc ggcctccttc ggg
//
LOCUS       DASPRR010000197        19840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1174543, whole genome shotgun sequence.
ACCESSION   DASPRR010000197 DASPRR010000000
VERSION     DASPRR010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19840)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19840
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174543"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..298
                     /locus_tag="VEL68_07645"
     CDS             77..298
                     /locus_tag="VEL68_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HXZ35875.1"
                     /translation="MEERRCLYLKVGDWVVHHKYPEWGAGRVVEERNSSVVGGLSLVK
                     VAFFDGQTRVFDNNFKNVTCCYHAGLRRR"
     gene            complement(470..1738)
                     /locus_tag="VEL68_07650"
     CDS             complement(470..1738)
                     /locus_tag="VEL68_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35876.1"
                     /translation="MKEKNPSPKKKSGPISPETENLVKEAIPQMSSWAEDPDLFLSRL
                     GTIPTEEWEELFLSLIRSQSEEVYPFIQQMVGREERIDLALASSLGHWNAPEAGILLH
                     RLASKTSSKTVLKGIRKSIFRLKTQGIDVQEIGDSSPAVFRPPQAIPSEGFVSSLDST
                     GTRFIWLVRPQLPQGVMVFHALVSDTQGMIDFQSFEASRKKFHQYLEEFRKNVPWEII
                     EAAPDYCLGLMNEAAEINQKREQIPPGDFLQGRSLMGTSRPLPLKPLIYQFLNEEEWK
                     GRSDLLDRSPSLFQLPYFQDWFLEEQESKKYLGLLKEASESPLVLSPFQKEARFIDIY
                     RQAVSELFDPGRKSLYRRRLEEMAYFLWKTGKESDAQMTLTAALAMESEGGVFSTHPF
                     LFELVKRSLTAALEEEARKAKKEDDLLIKP"
     gene            complement(1784..3484)
                     /gene="recJ"
                     /locus_tag="VEL68_07655"
     CDS             complement(1784..3484)
                     /gene="recJ"
                     /locus_tag="VEL68_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXZ35877.1"
                     /translation="MKWQVCPAHGGPENFPLRELGISPLTCQILRNRGIASPEEARRF
                     LHPTLSDLPDPLAMKDMETAVRRIVQALRQGEKIHLFGDYDVDGVTAAAVLFLFLKKA
                     GGNVDFTLPHRLREGYGLNLEAMKKIHSSGSRLVITADCGISSREEVRWSQEQGMDVI
                     VTDHHEIPEVLPPALAILNPKQRDCAYPFKGLAGVGVAFNLVIALRSFLRQEGFFSTG
                     NGPNLKEYLDLVALGTVSDVVPLTGANRILVKYGLAELTRAHRPGIAALREVSGMGAG
                     PVDSTGINFRLAPRMNAAGRLEEARDVVHLLVTEDPGEAKRIAVHLNELNSLRQRMEE
                     KVLAEAKEMIGLHEERKSIVLASPNWHPGIIGIVASRLTEEYHCPTILIALKEERGKG
                     SGRSIAAFSLFEGLKACQEWMDGFGGHEQAAGLVIRAECIPDFARAFEEVVAARVREE
                     DSIPCLTLDAFARLEQLNESFLSELEALAPFGAGNPEPVLGLEDLAILGSRPVGRGHL
                     RIRIKEGKQTREAIGFGMGPWHPLAGEGMKMAFSPQINIYQGNRNLQIRVLDLKPGES
                     "
     gene            3842..4258
                     /locus_tag="VEL68_07660"
     CDS             3842..4258
                     /locus_tag="VEL68_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35878.1"
                     /translation="MKRFLAPILIFSSLLFLMGMGGAGGSAPADKIPSPEKNFGVKVT
                     DREGFQTSLSQFSQEGKVFLTGKRGSATVTIPFEKISQIQLLSAEGNDVQAKLDLRNH
                     ESLDIKVDKRAKFYGKADFGTFQIDAKDLKTVSFLP"
     gene            4356..5054
                     /locus_tag="VEL68_07665"
     CDS             4356..5054
                     /locus_tag="VEL68_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ35879.1"
                     /translation="MPPKKVSRKKLLKEPDEFISTTGQVIQFVKGHQRQVMRVALIVL
                     LVLVAGAGTFTYLRWQEGKALAVQREGLMIYQSAYQASLENPAAEKKEEYKKALDKFK
                     ESLAIYGWGKTAQMTQIFIGNSYAALKEYAQAEAAYSQSLDGPFRPVGLNGLAYLFEA
                     QGNYSKSLEYYQKNLEEATSPYRLESLMGAARCYEILKQTPKALEIYEKALPQASKTA
                     LADFIRWKIGELKG"
     gene            5061..6851
                     /gene="iorA"
                     /locus_tag="VEL68_07670"
     CDS             5061..6851
                     /gene="iorA"
                     /locus_tag="VEL68_07670"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HXZ35880.1"
                     /translation="MEKRILLSGNEAIARGAFESGVKVAAAYPGTPSTEVLENLGTYS
                     GAHVEWSPNEKVALETAMGAAFGGARALAVMKHVGVNVAADPLFTLSYTGLRGGLVLV
                     TADDPEMHSSQNEQDNRHYARFAKVPMLEPSDSQEAKDFTKIAFDLSEKFDTPVMLRT
                     TTRVSHSKSIVHLDEPVIPPVKPGLIRDPVKLVMLPANARKRHPVVEKRMEEAARFAE
                     TFPENRIEWGDQKVGIITSGISYEYAKEVMPEASFLKLGMVYPLPKEMIKSFSSRVEK
                     VYVVEELDPFIEEQVRALGLVVEGKSSFPLLGELNPLLVEKGLKNLPSREKEEPVQKL
                     PARPPIMCPGCPHRGVFSVLNKLKLFVTGDIGCYTLGFLPPLSAIDTCVCMGASIGHA
                     MGLDKALGEEALGKVVAVIGDSTFLHSGITGLLNVAYNRGAVTVIILDNHTTAMTGRQ
                     DHPGTGCTLRGEETFEVDLVKLAKALGVKHVKAVNPYDLAAVERVIKKEVQRPEPSVV
                     ISRAPCVLSRRERVVSGKPLSVDSDFCAGCRSCLRLGCPAVAWVKEEGTNVQGKRRKG
                     RAEIDAFLCNGCTLCAQVCKFGAIKGNDGK"
     gene            6841..7419
                     /locus_tag="VEL68_07675"
     CDS             6841..7419
                     /locus_tag="VEL68_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXZ35881.1"
                     /translation="MENKVTNILLVGVGGQGIILASEIMADVFMEAGYDVKKSEVHGM
                     AQRGGSVSTHVRFGPKVYSPLIKEGEADIFMAFEELEALRYLSYLGLRPAILLNEQRL
                     NPPGVSLGNEDYPADVKEVLSRRAGKFKRIPARDLAVRSGDVRAVNVVLLGALHSFLS
                     IPEKTWTDQILKRFPPKAGPVNLEAFRLGKAA"
     gene            7445..8365
                     /locus_tag="VEL68_07680"
     CDS             7445..8365
                     /locus_tag="VEL68_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ35882.1"
                     /translation="MARPILWLFLFFFLCGGASAQEEGKRVVDEKAQMGLGDYFFEDG
                     DFYRAITEYKRFLFFFPESLRAEEALWKIASSYFQGKKWDEALSAADDLLKKHPSSPW
                     APQAILLKGRCWMEKKEFSQARHYFLLAREMAAGTGIAQEAQWQTAVSFLREERWKEA
                     AAEFRKVEASSRLYPRAEYWAQGLERIEQIPQKSPTTAGFLAILPGAGHLYCERYRDA
                     GVAFGLNAAFIWGMVEAFKHENYVVGGILTFFELGWYSGNIYSAVSSAHKYNRNKKKE
                     YLDNLEKEDRFSVGISFREKQPFFSFRYVF"
     gene            8373..8861
                     /gene="yidD"
                     /locus_tag="VEL68_07685"
     CDS             8373..8861
                     /gene="yidD"
                     /locus_tag="VEL68_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXZ35883.1"
                     /translation="MVFFLFCGCLSGGWVLAGEGRWEEPWPKRPPGPGPESQTPSTSL
                     SPGQKAAQGVLRLFQEYISPVDGDRCPCYPTCSQYSVEAIRKHGVWIGLVMTFDRLIH
                     ESSEIRQAPLVKVYGSYRYYDPVENNDFWWNKGSIQRSAISDQPSAVSKERQEPQLTA
                     ER"
     gene            8892..10193
                     /locus_tag="VEL68_07690"
     CDS             8892..10193
                     /locus_tag="VEL68_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXZ35884.1"
                     /translation="MLFDEEFETLPREALGALQLKRLAATAERVYATVPFYKKKFDEY
                     GIKPSSLKTLKDLERLPFTTKIDLRDNYPFGLFAVPMEQVVRIHASSGTTGKPTVVGY
                     TRRDINTWAELMARSLAAAGAHKNDIIHNAYGYGLFTGGLGVHYGAEKLGASVIPISG
                     GNSKRQIIIMQDFGSTVLTCTPSYALFLAETAEEMGVDFKKLKLKVGIFGAEPWSERM
                     RDEIERKLGIQAIDIYGLSEVIGPGVSIECIEAKRGLHIFEDHFIAEIIDPQTKEVLP
                     YGQKGELVFTTITKEAFPLIRYRTRDISVLYPEPCKCGRNHVRMERVSGRSDDMLIIR
                     GVNVFPSQIESVLMNIEGVEPHYLLFVDRQGNLDTLDLQVEVNEKVFSDEVKNLQSLG
                     RRIEKEIKDLLGVSVGVKLVEPKTIQRSEGKAQRVIDKRKI"
     gene            10307..10738
                     /locus_tag="VEL68_07695"
     CDS             10307..10738
                     /locus_tag="VEL68_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXZ35885.1"
                     /translation="MKVEQISVFLENKPGALTEVTRILGEAGVNIRALSLADTKDFGI
                     LRLIVNDNEKAKSVLGTRGFTVRKTEVVAVEVPDRPGGLADILRCLSDSGINVEYLYA
                     FVQQSGANAIIIFRFDETDRAIEVLSKNRVKILEGKKVYTL"
     gene            10785..11993
                     /locus_tag="VEL68_07700"
     CDS             10785..11993
                     /locus_tag="VEL68_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35886.1"
                     /translation="MNLKGFVVGVGLGACLMGSILPAMGAEKAPYVIGAVFSVTGGAS
                     FLGEPERNTAKMVEEWVNGAGGIQGHPLNVIVEDSKSDEGQAVLAVRKLLDKDKVLAI
                     IGPSTTGESMAVVPIMEKAKTPLISCAAGLSIVTPKEEMDRILAAKTFEMPKKQNPWV
                     FKTAQTDTSAVEAIYDFMKKKMITRVAIITVTNAFGDFGRQELKRAAPLYGISVVADE
                     RYGPKDSDMTAQLTKIKGTDAQALVNWSVGPPQVLVTKNWKQLGMNIPLYQSHGFGSK
                     RNIELAGGAAEGVLCPLGHIIVAEKVKADQPQKAVIMKYKTEYEKRFKTDVSSFGGYA
                     HDALYLLVDALKTVGPDKGKVRDFLETKVKNWPGVSGVFNLSPQDHTGLNKDAFVMIT
                     VKNNDWTFAD"
     gene            12272..13165
                     /locus_tag="VEL68_07705"
     CDS             12272..13165
                     /locus_tag="VEL68_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35887.1"
                     /translation="MTFGSQLIQYLFTGLTLGSIYALVALGFTMIYNATGIINLAQGE
                     FVMLGGLVMVFFAGVLKMPLYLGFLLTILVVTLVGVIFERLAIHPLKEASLITLIMIT
                     LAGSILFRGGAMFLWGKDPYALPPFTQGRPIQFLGASLQAQIFWVLGLTVFAVAGVHF
                     FFNRTLIGKAITACAFNPLAARLMGINVRKMVLLSFALSAAVGGVAGAIITPITLMEY
                     DRGPLLALKGFAAAVLGGLGSGIGSILAGFIIGILESLGAGLISSGYKDAIALVVLLI
                     ILWAKPSGILGNEEESGLKRF"
     gene            13271..14215
                     /locus_tag="VEL68_07710"
     CDS             13271..14215
                     /locus_tag="VEL68_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35888.1"
                     /translation="MKPKDLYIVFIIALVIGVFPWLTGRNEYFISLLVFMGINGMITL
                     GLSLLMGYAGQISLGHAAFFGLGAYSSGILTVHYGLSPVAGFLFGILLSAAVAYLVGK
                     PTLRLKGHYMAVATLGFGEIVFILFNELSFLTGGPSGLSGIPALAVGDLSLEGTAYLY
                     LVWGLVLLLLLFSLNLINSRVGRALRAVHGSELAAGAMGVDASLYKIQVFVLSAVYAS
                     MGGSLYAHFISFISPSSFSLMFSILLLMMVVVGGTETIWGAILGAAVLTLLPEYLRGL
                     EDFEVLAYGAILMAVLLFMPKGILEGFQRLWARAQGGS"
     gene            14222..14998
                     /locus_tag="VEL68_07715"
     CDS             14222..14998
                     /locus_tag="VEL68_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35889.1"
                     /translation="MSSEILNIQGLQKYFGGLRAIDDLGFAVSEGQIKSIIGPNGAGK
                     TTLFNLITGIFPPTQGMFTFAGKILNGLKTHEIARLGISRTFQNLELFPNMNSLENVM
                     LGCHCHTRAGIFRAGLRLRGMRQEEEKIREKAMEELRFMGLEEKALLGAKSLPLGEQK
                     LLEIARALATQPRLLLLDEPAAGLNIRETEALSESILRIRGRGITVMLVEHDMSLVMG
                     ISDEVLVLNYGKKIAEGQPREIQRNPGVIAAYLGEEAGNA"
     gene            14991..15725
                     /locus_tag="VEL68_07720"
     CDS             14991..15725
                     /locus_tag="VEL68_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35890.1"
                     /translation="MLRVESVSAFYGVIQALRNVSLHVNSGEMVALLGANGAGKTTLM
                     KVISGLRPLGKGRLFFSGQNIAGLPAERVLRLGVGQVPEGRQVFAPLSVLDNLILGAY
                     VRFKGEAKKEVYKDLDSIFEMFPVLKERQKQRAGTLSGGEQQMVAIGRTMMSRPKLLL
                     LDEPSLGLAPLVANGIFQTIRLLRDRGTTILLVEQNAKAALKVADRGYVLETGRIILE
                     GETEDLLNNKEVQRAYLGKEYREVWE"
     gene            15739..17037
                     /locus_tag="VEL68_07725"
     CDS             15739..17037
                     /locus_tag="VEL68_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXZ35891.1"
                     /translation="MIWDRKTECMPREEIEQLQLERLQSTLNRVYRNVAFYKKRFDRL
                     KIGPDEIQSVQDLAQFPLTMKEDLRESYPYGMFALPLREVVRIHSSSGVTGKPTVTGY
                     TKNDLHHWSQLTARVLAAGGVTKDDVVQITFKYGLFTGAFGLHYGAELIGASVIPMST
                     GNTRKQALIMQDYKTTALVSTPSYALLLARYLEKEGINPQSLSLKRGLFGGEPCPESM
                     RKEIQERLLIDAMDNYGVSEVMGPGVSAECERRNGLHLYEDHFIPEIIDPQTLKVLPP
                     GTEGELVLTTLTKEAFPVVRYRTRDITSLDYSLCPCGRTLVRMRKVLGRSDDIIVIKG
                     VKVFPSQVESILLEVEGVEPRYQILVEQVEGADSMELKVEVNEKVFSDEIKNLQNISN
                     QIERKLREIVGISAKVKLVAPESLQPTDGKMVKVIDRRKK"
     gene            17155..18165
                     /locus_tag="VEL68_07730"
     CDS             17155..18165
                     /locus_tag="VEL68_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ35892.1"
                     /translation="MKNFVSLLSAVLVFGFSMTGAFAQAKPVEFSYSIFFPATHKHTI
                     LAGEWGKEVEKRTGGRVKITIFPGGTLTPAATCYDGVVKGISDIGTSVLAYTKGKFPL
                     TEVIDLPLGYTNGIMATSLTNQYFAKFKPKEFDEVKILYLHAHGPGILHTKKAVSKLE
                     ELKGLRIRCTGTVTEIVKALGGVPMSMPMGETYDALNRGMVEGSMAPIESLEGWKWGE
                     VVKFTTESFGAAYSTAFFVAMNKEKWNALPADIQKTIETVNAEWAQKTGNLWDEVDKS
                     GREFSLKLGNKIIPLSKEENQRWATAVKPVLEEYVRRMKEKGLPGEEALKFCLEGLKK
                     QR"
     gene            18253..18735
                     /locus_tag="VEL68_07735"
     CDS             18253..18735
                     /locus_tag="VEL68_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ35893.1"
                     /translation="MDGLLRFIFRLSQGGNQVAGVSLTLVMGLTVADVILRILGRPIV
                     GAFELVAFLGAIAIGFSIPFTSWVRGHIFVDFLILKFSLKARKGIHIFTRLIGLGLFG
                     LAGWNLWKMGADLQRSGEVSPTLHVPFYPVVYGIGMVCLIQVLVMIADILKVLKEQYE
                     "
     gene            18728..>19840
                     /locus_tag="VEL68_07740"
     CDS             18728..>19840
                     /locus_tag="VEL68_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ35894.1"
                     /translation="MNEVMAGLVGLILVLALFFTGIELGFAMAVVGFLGFSYVVSLKA
                     GLNLLAKDVFDVLSSYGFTVIPLFIFMGQLAFNAGIAKRLYETAYKFFGHIPGGLAMA
                     TVGGATAFKAICGSSPATAATFASVAVPEMDRYGYSKKLSTGIVASVGTLGILMPPSV
                     TLIVFGIITEQSIGKLFLAGLIPGLLIAFFFILIIWVWCRIDPALGPKGQKSSWKDRI
                     SSLPEVLWVGIIFVLVIGGLMNGFFTPTEAGSIGTFAVLLLSLVKKDLTWRGYVKSIQ
                     ESLRTACMVLMLIVGSTLLGHFLAVTKIPMQAADWVIALNFPKELTIVIIALIYLLGG
                     SFIDDLAFMILATPIFYPVVLKLGYDPIWFGMIIGIT"
BASE COUNT         4407 a         5220 c         5759 g         4454 t
ORIGIN      
        1 gcccggcaag cgaatcccat gaaatggcct gatttttccg cttgcggaat aggtgggagt
       61 ttgatataag ccaattatgg aagaaaggcg atgtctatat ctgaaagtgg gggactgggt
      121 cgttcatcac aagtacccgg agtggggtgc gggacgggtg gtggaagagc ggaattcctc
      181 cgtggtaggt ggcctctccc tcgtgaaagt cgccttcttc gacgggcaga cccgtgtctt
      241 tgacaacaac ttcaagaacg taacctgctg ctaccacgct ggcctacggc gacgttaatc
      301 cttcctcatt aaagaattcc cggaataagc aaaaaccctg atactctttt tatccaattg
      361 ccttcgcctg ggctgggtcg ggcaggaatc cttttctctt acccgcaaat gtcaggctct
      421 atgagtctcc cagcatggcc gggggtaacc tttaggaaag taggatcggt tagggtttga
      481 tcaataaatc gtcttctttt tttgctttgc gggcttcctc ttcgagcgcg gccgtcaggc
      541 tgcgcttcac caactcgaaa aggaaggggt gggtggagaa gacccctcct tccgattcca
      601 tggccagggc ggcagtcaaa gtcatctggg cgtcactctc tttccccgtc ttccagagga
      661 aataagccat ctcttccagt ctccggcggt aaagggactt ccgccccgga tcgaaaagct
      721 cgctcactgc ctggcggtag atgtcgatga aacgggcctc tttctggaag ggagacaaga
      781 ccagagggct ttcggaggcc tctttcagga ggcccaggta ttttttgctc tcctgctcct
      841 ccaggaacca atcttggaaa taaggcaact ggaataggga aggggagcga tccagcaggt
      901 ccgagcggcc tttccattct tcctcgttca aaaactggta gatcaggggt ttcaggggca
      961 acgggcggga ggtccccatc agcgagcgcc cctgcaagaa atccccggga gggatttgct
     1021 cccttttttg attgatctcc gctgcctcgt tcataagtcc cagacagtaa tcaggggcag
     1081 cctctatgat ttcccaggga acattctttc ggaactcttc caagtactgg tgaaatttct
     1141 tgcgggaggc ttcaaaactc tggaaatcga tcatcccctg agtgtcgctc accaaagcat
     1201 gaaagaccat aaccccctgc ggaagctgag ggcggaccaa ccagatgaat cgagttccgg
     1261 tggagtcgag ggaactcaca aagccctcgg agggaatcgc ctggggaggg cgaaagacag
     1321 ccggggatga gtctccaatc tcttgaacat cgatcccctg ggttttcaag cgaaaaatcg
     1381 atttgcggat gccctttaga accgttttcg aggaagtctt ggaggctaga cgatggagaa
     1441 ggatcccggc ttcaggagca ttccaatggc ccaagctgct ggccaacgcc aggtcgatcc
     1501 tttcctctcg ccccaccatc tgctggatga aagggtagac ctcttcactt tggcttctta
     1561 tgagagagag aaaaagttcc tcccattctt cagtggggat ggttcccaat cgggataaaa
     1621 aaaggtctgg gtcttcggcc caggaagaca tctgggggat ggcctctttt accagattct
     1681 ccgtttccgg cgaaatgggg cctgattttt tcttagggga tgggttcttt tccttcatgg
     1741 tgccctcatt cattttgaag tctgggattc gcctttttcc cgcctatgat tcccctggtt
     1801 tgaggtccag gaccctgatc tgcaaatttc gatttccttg atagatgttg atctgaggag
     1861 aaaaagccat cttcatccct tcgccggcaa gggggtgcca gggtcccatg ccgaatccga
     1921 tcgcctctcg ggtctgtttt ccctctttga tacggatccg caggtggccc cggcctaccg
     1981 gccgggagcc gagaatggcc aggtcttcca ggcccagcac cggctccgga tttcccgccc
     2041 cgaaaggcgc cagggcttcc agttccgata gaaaggattc gttgagctgc tccagtctgg
     2101 caaacgcgtc gagggtcaga caggggatcg aatcttcctc tctcacccgg gcagccacaa
     2161 cttcctcaaa ggcccgggcg aaatcgggaa tacactcggc cctgatcacc agcccggccg
     2221 cttgttcatg gcctccgaag ccatccatcc actcctggca ggctttcaaa ccttcaaaga
     2281 gggaaaaggc agcaatggac cggccagagc cttttcccct ttcttctttc agggcgatca
     2341 ggatcgttgg gcagtggtac tcttccgtga gacgggaagc gacgatgccg atgattccag
     2401 gatgccagtt cggcgaagcc aggacgatcg attttctctc ctcgtggagg ccgatcatct
     2461 ccttagcctc agcaaggacc ttctcctcca tccgctggcg gagggagttc agttcgttca
     2521 ggtgaacggc aatccttttt gcttccccag ggtcctccgt cactaggaga tgaacgacgt
     2581 cccgggcttc ttcaagcctg ccagcggcgt tcattcgagg ggccaggcga aagttgattc
     2641 ccgtggaatc taccgggcca gcccccatcc ccgaaacttc tctgagggct gcaatcccag
     2701 ggcgatgggc ccgggtaagt tcggccaacc catacttgac taaaatgcga ttggccccgg
     2761 tgagggggac cacatcgctt accgttccca aggccaccag atcgaggtat tccttgaggt
     2821 tgggaccgtt tcctgtagag aaaaaacctt cctgacggag gaagcttcgc agggcgatga
     2881 ctaggttgaa agccactccc acccctgcca gtcctttgaa agggtaggcg cagtctcgct
     2941 gtttcggatt gaggatggcc agggctggag ggaggacctc cggaatttca tgatggtcgg
     3001 taacgattac atccatccct tgttcctggg accagcggac ctcttcccgg ctgctgattc
     3061 cacagtccgc ggtaatgacc agacggcttc ccgaggaatg gatttttttc atggcctcca
     3121 ggttcagacc ataaccctct cgaaggcggt ggggaagcgt aaaatcgacg ttgcctccgg
     3181 cttttttgag aaaaagaaaa aggactgcgg cggccgtgac gccgtccaca tcataatcgc
     3241 caaagagatg gatcttctcg ccctgccgga gggcttggac gatccgccgg accgccgtct
     3301 ccatgtcttt cattgccaga gggtcgggga ggtcggaaag ggtgggatgg aggaaccgcc
     3361 ttgcctcctc cgggctggcg attccccgat tgcggaggat ctgacaggtt aagggagaga
     3421 ttcccaattc ccgcagggga aaattttccg gccctccatg ggccgggcag acttgccatt
     3481 tcatgcgaga aggatgttgc aaccgcgttc tatttctcgt tgaattcata tttttcatgg
     3541 gcgcgaatct ttcaccaaaa tagcgaaggg atggggatcc tgtcaatcag atatttggcg
     3601 gtatttggat ttgcccactc ctcttcctgg taagagaccc gtgaattcaa agggctggtg
     3661 ttacatctgg gccataccat gcgtcctgcg gccttatcgc acctggacga ggatttagag
     3721 ttgtcatttt cagttaaaag tgttatgaaa ataatgaatg aatcaattgc attagaggat
     3781 caaagcgggt tgattctggc tttttgaagg ctaccaagac tcattccggg gaggcagagc
     3841 gatgaagagg ttcctggctc cgattctgat ctttagctcc cttctcttcc tcatgggaat
     3901 gggaggggcg ggcgggtctg ccccggccga taagattccg tctccggaga agaatttcgg
     3961 ggtgaaggta accgaccggg aaggattcca gacttcccta tcccagttca gccaggaggg
     4021 aaaggttttt ctcaccggga agcggggaag cgcaaccgtc accataccct ttgagaaaat
     4081 ttctcagatt cagcttctaa gtgcagaagg gaatgacgtc caagccaaac ttgaccttcg
     4141 taaccatgaa agcctggata tcaaagtgga caaaagggcg aagttttacg gaaaagcgga
     4201 ttttggaact tttcaaatcg atgcgaaaga cctgaagacc gtcagcttcc taccctagtc
     4261 tttcaacccg tcctcccaat cggaaggtca attccccaag aaaagcaggg ggagtggcgg
     4321 atttcatttt cggagttaat aaggagaagg ttggaatgcc acccaaaaag gtttcccgga
     4381 agaagttgct gaaagagccg gacgagttta tttccacaac agggcaggtc attcagtttg
     4441 tgaaagggca ccaacgccag gtgatgcgcg tggcgctcat tgttctcctg gtcctcgtgg
     4501 ccggagcggg cacctttacc tatctgcgct ggcaggaagg gaaggccctg gccgttcagc
     4561 gcgaaggcct gatgatttac cagagcgcct accaggcctc cctggagaac ccggctgcag
     4621 aaaagaagga ggaatataaa aaggccctgg acaaattcaa agaatctttg gccatttatg
     4681 gttgggggaa aacggctcaa atgacgcaaa tttttatcgg taactcttac gccgctctga
     4741 aagagtacgc ccaagctgag gcggcttatt cccagagcct ggatggacct ttccggcccg
     4801 taggcctgaa cgggctggcc tatcttttcg aggcccaggg gaattattcc aagtccctgg
     4861 aatattatca gaagaacctg gaggaggcca caagtcctta ccgcctggaa agcctgatgg
     4921 gagcggctcg atgttatgaa atcctaaagc aaacccccaa agctctggaa atttacgaga
     4981 aggctctgcc ccaggcctcc aagaccgccc tggccgactt tatccgctgg aagatcggcg
     5041 agctgaaagg ataagggatg atggagaaaa gaatcctcct atccggaaat gaggccatcg
     5101 cgcgaggggc tttcgagagc ggggtaaagg tggcggccgc ttacccggga acccccagta
     5161 cggaggttct ggaaaacttg gggacttact cgggggccca cgtggaatgg tcgccgaacg
     5221 agaaggtggc cctggaaaca gccatgggag ccgctttcgg cggagcccgg gccttggcag
     5281 tcatgaaaca tgtgggcgtg aatgtggccg cagaccccct ctttaccctt tcctacacag
     5341 gcctgcgggg agggctggta ttagtaaccg ccgatgaccc ggagatgcac agctcccaaa
     5401 acgagcagga caaccgccac tacgcccggt tcgccaaggt tcccatgctg gaacccagcg
     5461 atagccagga ggccaaggat ttcaccaaaa tcgcctttga cctgagcgaa aaattcgaca
     5521 ccccggtaat gctccggacg acaacccggg tgtcccactc caaatcaatc gttcatctgg
     5581 atgaacccgt tatcccgccc gtgaagccgg gactgatcag agacccggtc aaactggtca
     5641 tgctccctgc caatgcccgt aagcgccacc ctgtggtgga aaaaaggatg gaagaagcgg
     5701 cccgtttcgc cgagaccttc cccgagaacc gaatcgagtg gggcgatcaa aaggtgggga
     5761 tcatcaccag cggcatctcc tacgagtatg ccaaggaagt gatgcccgaa gcctccttcc
     5821 tgaagctggg aatggtttac cccctgccga aggagatgat taaatctttt tcttcccggg
     5881 tggagaaggt ttacgtcgtg gaagaattgg accccttcat cgaagagcag gtccgggctc
     5941 tgggtttggt ggtcgaaggg aaatcgtctt ttcccctcct gggagaactg aaccctctcc
     6001 tggtggaaaa aggtttaaag aatctcccct ccagggagaa agaagaacct gtccagaaac
     6061 tgccggcgcg tccccccatc atgtgcccgg gatgccccca tcggggggtc ttttcggttt
     6121 tgaataaact gaagctcttt gtgaccggag acatcggctg ctatactctg ggatttcttc
     6181 cgcctctcag cgctatcgac acctgcgtct gcatgggggc ttctatcggc catgccatgg
     6241 gtctcgacaa ggccctaggg gaagaggccc tgggcaaggt ggtggcagtc atcggggact
     6301 ccacttttct tcattccggg atcaccggcc ttctcaacgt ggcctacaat cggggagcgg
     6361 tgacggtgat catcctggac aaccacacca ccgccatgac cggtcggcag gatcacccgg
     6421 gaaccggttg cactctgaga ggggaggaaa ctttcgaggt ggacctggtc aagctggcca
     6481 aggccctggg ggtgaagcac gtgaaagcag taaatcccta tgaccttgcc gctgtggagc
     6541 gggtgatcaa gaaggaggtt cagcgtccgg agccctcggt ggtcatctcc cgggctccct
     6601 gtgtcctttc ccgccgggag agagtggtca gtggaaagcc tctttccgtc gattccgact
     6661 tctgtgcggg ctgccgatcc tgccttcggt tgggttgccc ggccgtggcc tgggttaagg
     6721 aagaaggaac caatgtgcaa gggaagcgga gaaaaggaag ggcggagatc gacgcgttcc
     6781 tctgtaacgg ctgcacgctc tgcgctcagg tgtgcaaatt cggggcgata aagggaaacg
     6841 atggaaaata aagtcaccaa tatactgctc gtgggggttg gcggccaggg aatcatcctt
     6901 gcttctgaga ttatggccga cgtattcatg gaagccgggt acgacgtaaa gaagagcgaa
     6961 gtccacggga tggcccagag gggaggcagc gtttccaccc acgttcggtt cggccccaaa
     7021 gtgtattccc ccctcatcaa ggaaggggag gcggacattt tcatggcctt cgaggaactg
     7081 gaagccctcc gttatctttc ctatctgggg ctccgcccgg ccatcctgct caatgaacag
     7141 cggctcaatc cgcccggcgt gtctctgggg aacgaagact atccggccga cgtaaaagaa
     7201 gtcctctccc ggcgggccgg aaagttcaag cgcattccgg cccgtgacct ggccgtcagg
     7261 tcaggggatg tgcgggcggt caacgtggtc ctcctgggag cgcttcactc cttcttgtcc
     7321 atcccggaaa agacctggac cgaccagatc ctgaaacgat tccctccgaa agcaggacca
     7381 gtaaacctgg aagcttttcg cttgggcaaa gcggcctgaa aaaatacctg tcgaggacct
     7441 ttccatggcc cgaccgatct tgtggctctt ccttttcttt tttctttgcg gcggggcctc
     7501 ggcgcaggaa gaggggaaaa gggtcgtaga cgaaaaggcc cagatgggcc tgggggatta
     7561 ttttttcgag gacggggact tctaccgggc catcaccgag tacaaacgct tcctcttttt
     7621 tttccccgag agcctgcgcg ccgaggaagc cttgtggaag atcgccagct cttattttca
     7681 agggaaaaag tgggacgaag ccctgtccgc cgccgatgac cttctgaaaa agcatccttc
     7741 ttccccctgg gcgccgcagg cgatccttct caaaggccgc tgttggatgg agaaaaagga
     7801 attctcccag gcccgccatt attttctcct cgcccgggaa atggctgccg gaactgggat
     7861 tgcccaggag gctcaatggc agacagcggt gagctttctg cgggaggagc ggtggaaaga
     7921 agctgcggcg gaattccgga aggtggaagc cagcagcaga ctctaccccc gcgccgagta
     7981 ttgggcgcaa ggcctagaac ggattgagca aattcctcaa aaatccccca ccaccgctgg
     8041 gttcctggcc attctccccg gagccggaca cctttattgc gaacggtacc gggatgcggg
     8101 ggtcgccttc ggcttgaacg ccgcttttat ttgggggatg gtggaagctt ttaagcatga
     8161 aaactacgtg gtgggcggaa tcttgacctt ttttgaactg gggtggtata gtgggaatat
     8221 ttacagcgcc gtctccagcg cccacaaata caatcggaac aagaaaaaag agtatcttga
     8281 caacctggaa aaagaggatc gcttttccgt gggcatatcc tttcgggaaa aacaaccatt
     8341 tttttctttc cgttatgtct tttaaaaaag ggatggtctt ctttctgttc tgcggctgcc
     8401 tgagcggagg ctgggttctg gcgggcgagg ggcggtggga agagccttgg cctaagcgcc
     8461 ctccaggtcc ggggcccgag agccagacgc cgtcaacctc cctttccccg gggcagaaag
     8521 cggcgcaggg cgtcctccgt ctctttcagg agtacatcag cccggtggac ggggatcgtt
     8581 gcccatgtta tcccacctgt tctcaataca gcgtggaggc aatccgcaag cacggtgttt
     8641 ggatcggcct ggtcatgacc ttcgatcggc tgattcacga gtccagtgaa atccggcaag
     8701 cgcccctggt caaggtctat gggtcctacc ggtattatga tccggtagaa aataacgatt
     8761 tttggtggaa taagggcagc attcagcgtt cagcaattag cgatcagcca tcagcggtca
     8821 gcaaagaaag gcaagagcct cagttgacag ctgaacgctg accgctgaaa gcttcttttt
     8881 ggagggattg tatgcttttt gacgaagaat tcgaaaccct tccccgggag gctctgggag
     8941 ccctgcaact caaacgtctc gcggctacgg cagagagggt ctatgccacg gttcctttct
     9001 ataaaaagaa gtttgacgag tacgggatca agccctcgag ccttaagacc ctgaaggacc
     9061 tggagcgtct gcctttcacc accaaaatcg acctgcggga caactatccc ttcggccttt
     9121 tcgccgtccc catggagcag gtggtgcgaa tccatgcctc ctcaggcacc acgggaaagc
     9181 ccaccgtggt gggatatacc cgccgggata tcaacacctg ggccgagttg atggcccgct
     9241 ccctggccgc tgccggggcc cacaagaacg acattatcca caacgcttac gggtatgggc
     9301 ttttcaccgg gggtctggga gttcactacg gggccgagaa gctgggggct tcggtcattc
     9361 ccatctccgg aggaaacagc aaacgtcaga tcatcatcat gcaggatttc gggtccacgg
     9421 tgctgacctg caccccctct tacgcccttt tccttgccga aacggcggag gagatggggg
     9481 tggatttcaa aaagctgaaa ttgaaggtcg gaatttttgg agccgaaccc tggtcggaga
     9541 ggatgcgcga tgagatcgag cggaagcttg gcatccaggc catcgacatc tacggcttga
     9601 gcgaagtcat cgggccggga gtgagcatcg agtgcatcga agccaaacga ggcctgcaca
     9661 tttttgagga tcattttatt gccgagatca tcgaccccca gacgaaagaa gtcttacctt
     9721 acggccagaa gggagaactg gtctttacca cgatcaccaa ggaagccttc cccctcatcc
     9781 gataccggac ccgagatatt tcggtccttt atcccgagcc gtgcaagtgc gggcgaaacc
     9841 atgtgcgcat ggagcgggtc agcgggagga gcgatgatat gctcatcatc cgtggggtta
     9901 acgtcttccc ctcccagatt gaaagcgttc tcatgaacat cgagggggtg gagcctcact
     9961 atctcctgtt tgtggaccgg caggggaacc tggatacctt ggaccttcag gtggaagtga
    10021 atgagaaggt cttctccgat gaagtcaaaa acctccagtc cctcgggaga aggatcgaga
    10081 aggaaatcaa ggacctcctg ggggtgagcg tgggggtgaa gctagtggag cccaaaacca
    10141 tccagaggag tgaagggaag gcgcagaggg tgatcgataa gagaaaaatc tagatgctcg
    10201 atgctggata ctggattcca ggtgcctaag gcatttccca ggtcctatat cctatatcca
    10261 gtataaaaca ttcagtatct ggccgtcggg aaactaggag gtcctcatga aagttgaaca
    10321 aatctcggtt ttcttggaaa acaagcccgg ggctctgacc gaggtaaccc gtatcctggg
    10381 ggaggctgga gtgaatatcc gcgccctgtc cctggcggac acgaaggatt tcgggattct
    10441 gcgcctgatc gtcaatgaca atgagaaggc caagtcggtt ctgggcaccc ggggattcac
    10501 cgtgaggaag accgaggtag ttgcggtgga agtcccagac cgtccgggcg ggctcgcgga
    10561 tatcctgaga tgcctgtccg attcaggaat caacgtggaa tatctctacg ccttcgtcca
    10621 gcagagcgga gccaatgcta tcatcatttt ccgttttgat gaaaccgacc gagctatcga
    10681 agtgctttcc aagaaccggg tcaaaatttt ggaaggaaag aaggtttaca ccctgtagcg
    10741 atttccatgc aaggggaggc atggaaattc ttgagaggag ggaaatgaat ctgaaggggt
    10801 ttgtggtagg agttgggtta ggggcctgtt taatggggtc gatccttccg gccatgggag
    10861 cggaaaaggc tccctacgtg atcggagccg tgttcagcgt gaccggaggg gcttctttcc
    10921 tgggagagcc ggaacgcaac accgcaaaaa tggtggaaga atgggtgaat ggagccgggg
    10981 gaatccaggg acatcctctt aatgtaatcg tcgaagacag caagagcgat gaaggccagg
    11041 ccgttctggc ggtacgcaag ctcttggata aggacaaggt cctggccatc atcggaccct
    11101 ccaccacagg ggagagcatg gctgtcgtgc ccatcatgga aaaagccaaa accccgttga
    11161 tttcctgcgc agccggcctt tccatcgtca ctcccaagga ggagatggac cgcatattgg
    11221 ccgccaagac ctttgaaatg cccaagaaac agaacccctg ggttttcaag acggcgcaga
    11281 ccgacacctc agcggtggaa gcgatttatg acttcatgaa gaaaaagatg atcaccaggg
    11341 tggcgatcat caccgtcacc aatgcgttcg gggacttcgg gcgccaggag ctgaagaggg
    11401 ctgccccgct ctatgggatc tccgtcgtgg ctgatgagcg gtatgggcct aaggattccg
    11461 atatgaccgc ccagctgacc aaaataaagg ggaccgacgc ccaagccctg gtcaactggt
    11521 ccgtgggtcc cccccaagtt ttggtaacaa agaactggaa gcaactgggg atgaatatcc
    11581 ccctctacca gagccatggg tttggatcga agaggaacat tgagctggct gggggagccg
    11641 ccgaaggggt gctctgcccg ctggggcaca tcatcgtggc ggaaaaagtg aaggccgacc
    11701 aaccgcaaaa agcggtgatc atgaaatata aaaccgaata cgagaagcgg tttaaaacag
    11761 atgtgtcgag cttcggcgga tacgcccacg atgccctcta tctccttgtg gacgccctga
    11821 agaccgtggg gcctgataag gggaaggtcc gtgatttcct ggagaccaag gtgaagaact
    11881 ggccgggggt cagcggagtt ttcaaccttt cccctcagga ccacacgggt ctgaataagg
    11941 acgcttttgt catgattacg gtcaaaaata atgactggac atttgccgat tgatgtttat
    12001 aataggggag atatcctcga agcagattgg gaaaagatgc cagactttga tcccatcgtc
    12061 attcagatgt tgaatcaacg cggggttttc ggacttgcgg ggaaaactgg agacctgcgc
    12121 ctttcttttt tcggctacta ttatttctaa agagcgctgc gggccttcga atcttattcc
    12181 ctattgaagg cctgtagcgc tctttgttta tccgggaacc gaatgttgac gaacgattga
    12241 aaattatgac tgatcggggt actgattttt tatgactttc ggcagccagt tgatccaata
    12301 cctctttacc ggattaaccc tgggcagcat ctatgccctg gtggcactgg ggtttaccat
    12361 gatctacaat gccacgggga tcatcaacct ggcccagggg gagttcgtca tgctcggcgg
    12421 gctggtcatg gttttctttg ccggagtcct gaaaatgccc ctttacctgg ggttcctcct
    12481 gaccatcctc gttgtaaccc tggtgggagt catcttcgaa cgcctggcca tccatcccct
    12541 gaaggaagcc tcgctcatca ccctgatcat gatcaccctg gccggatcga tccttttccg
    12601 gggaggggcc atgttcctgt gggggaaaga cccctatgcg ctccccccct ttactcaggg
    12661 acggcccatc cagttcttgg gagcctctct gcaggcccag attttctggg tgttgggcct
    12721 cactgttttt gccgtcgctg gggtccattt tttcttcaac cggaccctga tcggaaaagc
    12781 catcaccgct tgcgccttta atcctctggc ggcccgcctc atgggaatca atgtacgaaa
    12841 gatggttctt ctttctttcg ccctgagcgc cgccgtagga ggggttgctg gggcaatcat
    12901 tacccccatt acgctcatgg agtatgaccg ggggccgctc cttgcgctga aggggtttgc
    12961 cgccgcagta ctcggggggc tcgggagcgg aatcggctca attctggccg gtttcatcat
    13021 cggcatcctg gagtccctag gtgccggact gatctcctcg gggtacaagg acgccatcgc
    13081 cctcgtggtt ctgttgatca tcctctgggc gaaacccagc ggaatcctgg gaaacgagga
    13141 ggagagcggg ttaaaaaggt tttgagtttc aaggttcgag tttcgggtta agagcccacg
    13201 aaaatgagag ttaatttttt tcacacctga aacccgaagc tcgaaacccg aaactatttt
    13261 ttaggtggtc atgaagccta aagacctcta catcgttttt atcatcgccc tggtcatcgg
    13321 ggtcttcccc tggctcaccg gaaggaacga gtatttcatc agcctcctgg tgttcatggg
    13381 gatcaacgga atgatcaccc tggggctcag tcttctcatg ggttatgcgg gccagatttc
    13441 cttgggccat gcggctttct ttgggctggg ggcctattct tcggggatcc tgaccgtcca
    13501 ttacggcctg tcccccgtcg ccggttttct ctttggaatc cttctttcag cggccgtggc
    13561 ctatctcgtg ggaaagccga ctctgcggct gaaggggcat tacatggcgg tcgccaccct
    13621 gggttttggg gaaattgtgt tcattctctt caacgaactc tccttcctga ccggtgggcc
    13681 ctcgggcctc tccgggatcc ccgctctggc ggtcggggac ctgtccctgg aggggacggc
    13741 gtatctttac ctggtctggg ggctcgtcct cctcctcctt ctcttctccc tgaatttgat
    13801 caattcccga gtgggaagag ccctgcgggc cgtccacgga agcgagttgg cagccggcgc
    13861 catgggagtg gatgcttccc tctacaagat tcaggttttc gtgctgagcg ccgtctatgc
    13921 ctccatgggc ggaagccttt acgcccattt cataagcttc atcagcccca gctccttcag
    13981 cctcatgttt tctattcttc tcctgatgat ggtggtcgtg gggggtacgg agaccatttg
    14041 gggggccatc ctgggggctg ccgtccttac cctcctgccc gagtacctgc gcgggctgga
    14101 ggatttcgag gtcttagcct acggcgccat tctcatggcc gttcttctct tcatgccgaa
    14161 gggaattctg gaggggtttc aaaggctgtg ggcaagggcg caagggggat cgtagggaag
    14221 gatgagcagc gagattctca atattcaggg tctgcaaaaa tatttcgggg ggctgagggc
    14281 catagatgac ctgggttttg ccgtctccga gggccagatc aaatcgatca tcggccccaa
    14341 cggtgcgggg aagaccaccc ttttcaatct tatcaccgga atttttccgc cgacccaggg
    14401 gatgtttaca ttcgcgggga aaatcctgaa cgggctgaag actcacgaaa ttgctagact
    14461 gggcatttcc cggactttcc agaacctgga gctcttcccc aacatgaatt ccctggagaa
    14521 cgttatgctg gggtgccatt gtcacacgag ggcgggcatc tttcgcgctg gtcttcgcct
    14581 tcggggcatg aggcaggaag aagaaaagat tcgggaaaag gccatggaag aactccgttt
    14641 catggggctc gaagagaaag ccctcctcgg cgcgaaatcg ctccccctgg gagagcagaa
    14701 actcttggag atcgcccggg ccctggccac ccaaccaagg cttcttctcc tcgacgagcc
    14761 ggcggcgggc ctgaacatcc gggagacgga ggctctttcg gaatccatcc tgcggatccg
    14821 agggcggggg atcacggtta tgctcgtgga acacgacatg agcctggtca tggggatctc
    14881 cgatgaagtc ttggtcctca attacggaaa gaagatcgcc gaagggcaac cgcgggagat
    14941 tcagcgaaac cccggagtga ttgccgccta cctgggcgag gaggcaggga atgcttaggg
    15001 tggagagtgt cagcgccttc tacggcgtca tccaggcgtt gcggaatgtt tctcttcatg
    15061 tgaactcggg agagatggtc gcccttctgg gggccaacgg tgcggggaag accaccctga
    15121 tgaaagtcat ctccgggctt cgccccctgg gcaaggggag gcttttcttc agtggacaga
    15181 acatcgccgg gctgccggcg gaacgggtcc tgcgcctggg ggtcggacag gttcccgagg
    15241 ggagacaggt cttcgcccct ctttctgttc ttgataacct gattctaggg gcctacgtcc
    15301 ggttcaaagg ggaggccaag aaagaggtat ataaagacct ggattccatc tttgaaatgt
    15361 tcccggtcct gaaggagcgg cagaagcaga gagcgggaac cctctctggg ggagagcagc
    15421 agatggtggc catcgggcgg accatgatgt cccggcccaa acttctcctt ctggatgaac
    15481 cctctttggg actcgcccct ctggtggcca atggaatttt tcagaccatc cgccttctgc
    15541 gcgaccgggg aaccactatc cttcttgtgg aacaaaacgc caaagccgcc ctgaaagtgg
    15601 cagaccgtgg gtatgtttta gagacgggac ggatcatcct ggaaggggag acggaggacc
    15661 tcttgaacaa caaggaagtc cagcgagcct acctgggcaa ggagtaccgg gaggtgtggg
    15721 agtaaggggg gagggaaaat gatctgggac cgcaaaaccg agtgcatgcc ccgcgaggag
    15781 atcgagcaac tgcagctgga gcggctccag tccaccctga accgggtgta ccggaacgtg
    15841 gctttctaca agaagcggtt cgatcgactg aagattggcc cggatgaaat tcaaagcgtt
    15901 caggatttgg cgcagttccc actcaccatg aaagaggacc tgagggagag ttacccctac
    15961 gggatgttcg cccttcccct gcgggaagtg gtccgcatcc attcctcctc cggggtaacg
    16021 ggcaagccca cggtgaccgg ttacaccaag aatgacctgc atcactggag ccaactcacc
    16081 gcaagggtcc tagccgcggg aggggtgacg aaggacgatg tggtccagat taccttcaaa
    16141 tacgggcttt ttaccggagc tttcggccta cattacggtg ccgagctcat cggagcctcg
    16201 gtcattccca tgtccaccgg gaacacgcgg aagcaggctt tgatcatgca ggactacaaa
    16261 accactgctc tggtgagcac gccttcctac gccttgctct tggcccggta cctggaaaaa
    16321 gaggggatca accctcaatc cctctccctg aagagaggtc tctttggcgg agagccttgt
    16381 ccggaatcca tgcgcaaaga gattcaagag cggcttttaa tcgacgccat ggacaattac
    16441 ggggttagcg aggtgatggg ccccggcgtc tctgcggaat gtgaacgcag gaacggcctc
    16501 catctttatg aagatcattt tatccccgag atcatcgacc ctcagacgct gaaagtcctc
    16561 cctccgggca cggaagggga gttagtcctg accactctga cgaaagaggc cttccccgtc
    16621 gtccgctacc gcacccggga cataacttct ttggattatt ccctctgccc ctgcggccgg
    16681 actctggtgc gcatgaggaa ggtcttgggg cggagtgatg acatcatcgt catcaagggg
    16741 gttaaggttt tcccttccca ggtggaatcc atcctcctgg aggtggaggg ggtagagccg
    16801 cggtaccaga tcctagtgga acaggttgaa ggggcggatt caatggaatt gaaagtggaa
    16861 gtaaacgaaa aagtcttttc cgacgaaatc aagaaccttc agaacatctc caatcagatc
    16921 gagcgaaagc tacgagagat cgttggaatc agcgccaagg tgaagctagt ggcacccgaa
    16981 agcctgcagc cgacagatgg taagatggtt aaggtcatcg atcgaaggaa gaaatgatca
    17041 gcccggaatg gggaaagagg atatagaatc ccggtaccct cgaaacgcag aaaaacaatt
    17101 tagtcaaagg aggattgccg gggtaatcaa tttattgacc aggagggatt gtcgatgaag
    17161 aatttcgttt ccctgttaag tgctgtttta gtttttgggt tttccatgac gggggccttt
    17221 gcccaggcca agcctgtgga gttctcctac tctattttct ttcctgcaac ccacaaacat
    17281 acgatcctgg ctggggagtg gggcaaggaa gtagaaaaaa ggaccggcgg aagagtgaaa
    17341 atcaccattt tcccgggtgg gaccctgacc cctgcggcca cctgttacga tggagtagta
    17401 aagggcatat cggatatcgg gacctcggtc ctggcttaca ccaagggcaa gtttcccctg
    17461 accgaagtca tcgatcttcc cttgggatac accaatggga tcatggccac atccttgacc
    17521 aaccagtact ttgcgaaatt caaacccaag gagttcgacg aggtcaaaat tctctacctc
    17581 cacgcccatg ggcccgggat cttgcatacc aagaaggcgg tcagcaagct ggaagaactc
    17641 aagggtctga gaatccgctg tacgggaacg gtgaccgaaa ttgtgaaggc cttgggaggt
    17701 gttcccatgt ccatgcccat gggggaaacc tacgatgccc tcaaccgggg aatggtggag
    17761 ggctccatgg cccccatcga atccctggag ggatggaagt ggggagaggt ggtgaaattc
    17821 accacggaaa gtttcggggc tgcttacagc acggcctttt tcgttgccat gaacaaggaa
    17881 aaatggaacg ctttgcccgc agatattcaa aaaaccatcg aaacggtcaa tgccgaatgg
    17941 gcccagaaga cgggcaattt atgggatgag gtggacaaat cggggaggga gttttcccta
    18001 aagctgggca acaagatcat tcccctttcg aaagaagaga atcagagatg ggccacggcg
    18061 gtaaaaccgg tgcttgagga atatgttaga aggatgaaag aaaaaggatt gcccggggaa
    18121 gaggctttga aattctgtct ggagggcctg aagaagcaaa gataaccctg accggccagg
    18181 agagagaagg gggcgggcct tttttttcca taatcccggc ggaagggcag gggggaggaa
    18241 accgagaagc ttatggacgg tctactgcgt ttcatattcc gtttaagcca gggggggaac
    18301 caagtggctg gagtctcctt gactctggtc atgggattaa ccgttgccga cgtaatcctc
    18361 cgaattctgg ggaggcccat cgtgggcgcc tttgaattgg tggctttttt gggcgccatc
    18421 gcgatcggtt tttccattcc cttcacttcc tgggtgcgag gccacatttt cgttgatttc
    18481 ctgatcctaa aattttccct gaaagcaaga aagggaattc atattttcac ccgcctcatc
    18541 ggactcgggc ttttcggact cgccggctgg aacttgtgga agatgggcgc cgatttacag
    18601 aggtccggtg aagtctctcc gactcttcat gttccctttt atccggtggt ttatggaatc
    18661 gggatggtgt gcctgatcca ggttttggtg atgatcgccg acatcttgaa agtgctaaag
    18721 gagcagtatg aatgaagtga tggccgggct cgtcggcctg attctggtat tggctctctt
    18781 cttcaccggc atcgaattgg gatttgccat ggccgtcgtg ggtttcctgg gattcagcta
    18841 tgtagtttcc ctcaaagcgg ggctgaatct cttggcgaaa gacgtcttcg atgtcctctc
    18901 ctcttatggg ttcacggtga tccctctttt catctttatg gggcagctgg ccttcaatgc
    18961 gggaattgcc aagcggctgt atgaaaccgc ttataaattc ttcggccata tccccggagg
    19021 gctggccatg gctactgtag gcggagccac cgccttcaag gccatttgcg gctcttcccc
    19081 tgctaccgca gcaacctttg ctagcgtggc ggttccggaa atggaccgct acggatacag
    19141 caaaaagctt tccaccggaa tcgtggccag cgtgggcacc ctggggatcc tcatgccccc
    19201 gagcgtaacc ctgattgttt tcgggatcat cacggagcag tccatcggca aacttttcct
    19261 ggctggcctc atcccagggc tgctgattgc cttctttttc atcctgatca tctgggtgtg
    19321 gtgccgaatc gatccggcgc tgggccccaa ggggcaaaag tcttcctgga aagatcggat
    19381 cagttccctt ccggaagttc tgtgggtggg aatcatcttc gtcctggtca tcggcgggct
    19441 gatgaatggc tttttcactc ccaccgaggc cggcagcata gggactttcg ccgttctctt
    19501 gctttcccta gtgaagaaag acctgacctg gagggggtat gttaaatcca tccaggaatc
    19561 cctgcgaacg gcctgcatgg tccttatgct catcgtgggg tccacgctgc tgggtcattt
    19621 tttggccgtg accaaaattc ccatgcaggc cgcggactgg gttatcgcgc tgaattttcc
    19681 gaaggaattg actatcgtca tcatcgccct catttacctc ctgggtgggt ccttcatcga
    19741 tgacctggcc ttcatgatcc tggccactcc catcttctac ccggtggtcc tgaaactggg
    19801 gtacgatccc atatggtttg ggatgatcat cgggatcacg
//
LOCUS       DASPRR010000198         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_51817, whole genome shotgun sequence.
ACCESSION   DASPRR010000198 DASPRR010000000
VERSION     DASPRR010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51817"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..553
                     /locus_tag="VEL68_07745"
     CDS             314..553
                     /locus_tag="VEL68_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HXZ35895.1"
                     /translation="MAIAAYIFIETTQGKARGITREIIRIPGVKTAHSVTGPYDVVTF
                     VEADSISVLGDFIVTKIQAIPGVLRTLTNVVIDSE"
     gene            complement(680..1267)
                     /locus_tag="VEL68_07750"
     CDS             complement(680..1267)
                     /locus_tag="VEL68_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HXZ35896.1"
                     /translation="MSREQQGVILFLILFLCLLFFLTGPGPVKERETLLSPGKQEAPW
                     IPSGGKEFVLELEGSVERRGIIPAQPGVTVREALEKAGGIYGGLSLNEEDLGQKIERS
                     SRLTVLPGREGKGSVLVEAFPPAKLKVLSIPVSVNSATAEELDALPGIGPKTARAIVE
                     FREKYGKFSSPEDLLQVPGIGPKKLAALNPHITIP"
     gene            complement(1384..2619)
                     /locus_tag="VEL68_07755"
     CDS             complement(1384..2619)
                     /locus_tag="VEL68_07755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXZ35897.1"
                     /translation="MALIVQKYGGTSVGDLARIRNVAQRVAKTRREGNDVVVVVSAMA
                     GETDRLIRLAQQASEMPDLREYDSLISTGEQVSAAMLAITLQSMGISARSFLGPQIRV
                     LTDSAFSVARIREVETARLRRELKAERVPVVTGFQGVDPKGNITTLGRGGSDTTGVAV
                     AAALKADVCEIYTDVDGVYTMDPNICPDARKISRISYNEMMEMASLGAKVLQIRSVEF
                     AKKYNVPIHVRSSFNDKSGTWVVKEDEKMEKVLVSGVTLSKNEARITITRVPDKPGVA
                     SKIFNPISDAHIVVDMIIQNTSSDGSTDLTFTIPKGDLKKALKLVKKTAEEIKAQSVM
                     ADENIVKVSIVGAGMVSHAGVASKMFTALAKENINILMISTSEIKISCVVEAKYGELA
                     ARVLHQAFALEKGDLRKEG"
     gene            complement(2621..3148)
                     /gene="tsaE"
                     /locus_tag="VEL68_07760"
     CDS             complement(2621..3148)
                     /gene="tsaE"
                     /locus_tag="VEL68_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXZ35898.1"
                     /translation="MGPVFVPLQPFPWRSEILEIITDSPGQTLRLGRRLGEILFGGAF
                     IALVGSLGAGKTLLTKGIAGGLGVEDPGEVTSPTFVLVNEYRGRVPVYHIDLYRMESL
                     AEVEEIGWDEWIGGPGVTLVEWAEKMEDNLPEERVEVHLQWAGEEKRRLLFYGRGRMG
                     LQIIEELRHRWKKEE"
     gene            complement(3268..>3619)
                     /locus_tag="VEL68_07765"
     CDS             complement(3268..>3619)
                     /locus_tag="VEL68_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HXZ35899.1"
                     /translation="GVVLVLKGARSLVASPEGDLFINPTGNPGMASGGMGDILTGMIG
                     GFLAQGIPSLEAAKLGVYLHGLVGDYVAHLKGERGMLAMDLAEETPKVLRALGSAEGQ
                     VGDFSFPLRTEICY"
BASE COUNT          738 a         1074 c          932 g          875 t
ORIGIN      
        1 aggcgaccac cttcttcgac cgggagatga cccgggcggt cttttcgatc aggtcttctt
       61 tcaagagccc ccctttccaa tgcggaatgg agaatgtgga ataaatatcg tttttttctt
      121 tccctaccgg ggggaggtgt gatcattcta tcagagcgga tgattttagc aagggcattc
      181 aaaatggatg cacttcagct ttttctttaa atggggtaaa atggatttac cacaagagca
      241 cgggttgcga taatggaata aacagtcttt tttattccgc gttccgcatt ccgaattcaa
      301 aggagggggg aacatggcca tcgccgctta tatcttcatc gagactaccc aaggaaaggc
      361 ccgggggatt acccgggaga tcatccggat tccgggggta aaaactgccc attccgtcac
      421 tggcccctat gatgtggtta cttttgtgga ggcggattcc atatcggtcc tgggtgattt
      481 catcgtcacg aagatccagg ccattcccgg agttctgcgc accctgacga atgtggtgat
      541 tgactcggaa tagacgaatg gccgctcccg ggcattcgtc tcctcgtcac agggcactgg
      601 tttccggcca ccctttacgc cggtagacct ggatttcggg atttaggtcc aagacctccg
      661 acttgtggcc tgatcgcccc taggggatgg taatgtgcgg gttgagggcg gctagcttct
      721 tcggcccgat tccggggact tggagcaagt cctcgggaga ggaaaatttc ccatatttct
      781 cccggaattc cacgatggcc cgggccgttt tgggtccgat tcccggcagg gcatccaatt
      841 cttcggccgt ggccgaattg acggagacgg ggatggatag aactttgagt ttggcggggg
      901 ggaaggcttc tacgaggacg ctccccttcc cttctctccc gggcaagacg gtcagacgac
      961 tgctccgttc aattttttgc ccgagatcct cctcattcag agaaagtcct ccatagattc
     1021 cccctgcttt ttccaaagct tctcttaccg tcacccccgg ttgggccggg atgattcccc
     1081 gtcgctccac gcttccttcc agctccagga cgaattcctt tcccccggat ggaatccagg
     1141 gagcctcttg ctttccggga gaaagaagcg tctccctctc tttcacgggc cctggcccgg
     1201 tcaggaaaaa gagaaggcaa aggaatagga taagaaagag gattactccc tgctgctccc
     1261 gactcatgtt ttacccttcc ggtctcttag gaatttacat ccatggagaa atcctcggtc
     1321 cggccccgtc tcagcaggat cacgtctccg aatagggctc cttttccggc ggccccttgg
     1381 ccttcaccct tccttcctca aatccccttt ttccaaggcg aaggcctgat ggagaactcg
     1441 ggcagccagc tcgccatact tggcctcgac cacgcaagag atcttgatct ccgaggtgct
     1501 gatcattagg atgttgatgt tctccttggc cagggcggtg aacatcttgg aagccacgcc
     1561 ggcgtggctg accatccccg ccccgacaat ggagaccttg acaatgtttt catcggccat
     1621 gactgactga gctttgattt cttcggccgt tttcttcacc agcttcaaag cttttttgag
     1681 gtcccctttg ggaatcgtga aggtgagatc ggtggaccca tcggaactag tgttctggat
     1741 gatcatatcc accacgatgt gggcatcgga aataggatta aaaatcttgg aagccacgcc
     1801 gggtttgtcg ggcacgcggg tgatggtgat gcgggcttca ttcttgctca gagtgacgcc
     1861 ggagaccagc accttttcca ttttttcatc ctccttgacc acccaagtgc cgctcttgtc
     1921 gttaaaggac gaacggacat gaatgggaac gttatatttc ttggcaaact cgacggatcg
     1981 aatctggagc accttggctc ccagggaggc catttccatc atttcattgt aagaaattcg
     2041 ggagattttt cgagcgtccg ggcagatatt cgggtccata gtatagactc catccacgtc
     2101 ggtatagatc tcacaaacgt cggctttcaa agcggcggcc acggccactc ccgtggtgtc
     2161 cgacccaccc cggcccaggg tggtgatgtt tccctttggg tccactccct gaaagccggt
     2221 caccacagga actctttccg cctttaactc gcgccgcaaa cgggcggttt ccacttcccg
     2281 gatccgggcc accgaaaaag cgctgtcggt gaggacccgg atttgagggc ccaggaagga
     2341 cctggcggaa atccccatcg attggagggt gatggccagc atggccgcgc tcacctgctc
     2401 ccctgtggaa atcagactat cgtattcccg caagtcgggc atttcggaag cctgctgggc
     2461 caggcggatg agccggtccg tctctccagc catggccgaa accaccacca cgacatcatt
     2521 tccctcgcga cgggttttgg ccacccgctg ggccacattc cgaatccggg cgaggtcccc
     2581 caccgaggtt cccccatatt tctgcacaat cagggccacc ttattcctcc ttcttccatc
     2641 ggtgcctcag ttcttcgatg atttgcagtc ccatcctgcc ccgcccgtag aagagcagcc
     2701 ttctcttttc ttcgccggcc cactggaggt gaacctctac ccgttcttcg ggcaagttat
     2761 cttccatttt ttccgcccat tcaaccagcg tgactccagg gccgccgatc cattcgtccc
     2821 atccgatttc ctccacctcg gcgagactct ccatacgata aagatcgatg tggtaaacgg
     2881 ggacccggcc ccggtattcg ttcaccagca caaaggtcgg actggtcact tcccctgggt
     2941 cctcgactcc cagccccccg gcgattccct tggtcaggag ggttttgccc gcccccaagc
     3001 ttccaaccag ggctatgaag gcccccccga agagaatttc gcctaacctg cgacccagtc
     3061 gcagggtttg acccgggctg tcggttatga tttcaagaat ttctgatctc caagggaaag
     3121 gctgaagggg aacgaaaact ggccccattg cccaaagtcc gaatcttcat ggtcctatcc
     3181 atccttttcc gctttccggc ccttctacaa tcgcaacttg aaaaatagtc gaatctaggt
     3241 ttttttgatg gggggtgatt ttgtcattta atagcagatt tcggttcgca ggggaaagga
     3301 aaaatcaccg acttgacctt cagccgagcc caaagctcgc aggaccttag gcgtctcttc
     3361 cgccagatcc atagcgagca tgccccgttc ccctttcaga tgggctacat aatctcccac
     3421 tagtccgtgc aaatagacac ccagcttcgc cgcctccagg gaaggaatgc cttgggcgag
     3481 aaatcctccg atcattccgg tgaggatatc tcccattcca ccagaggcca tcccgggatt
     3541 ccccgtaggg ttgatgaaca ggtccccttc ggggctcgcc accagagagc gggccccctt
     3601 gagtaccagg accacccca
//
LOCUS       DASPRR010000199         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_535809, whole genome shotgun sequence.
ACCESSION   DASPRR010000199 DASPRR010000000
VERSION     DASPRR010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535809"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VEL68_07770"
     CDS             complement(<1..621)
                     /locus_tag="VEL68_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXZ35900.1"
                     /translation="MNFKDFWPWRKKAILTRAGEDLPYPTPPGKPRAFWNLVLFCLTL
                     ITTVLAGALQEGVNPLTHPEQIIRGIPFAVALMGILLAHELGHYLVARKNGVNVTLPY
                     FIPAPSFIGTFGAFIKMRSPARDRRMLMDIGAAGPLVGVMVCVPILILGLSLSEVKKI
                     EGQVGTNLGDSLLLWLISRWVVGEIPPGYDVIIHPVGFAGWIGLLVT"
     gene            complement(762..2147)
                     /locus_tag="VEL68_07775"
     CDS             complement(762..2147)
                     /locus_tag="VEL68_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ35901.1"
                     /translation="MNRRRDLGFLFNPRSIALVGVPRGFEPGRVFLQGLLEQGYSGKV
                     FPINPKADSIDGCRAYPSLKEVPGEIDLAILLLPAALVLPALEECGQKGVKIAVIYTS
                     GFAETGDEEGKKREAALLQVARKEGIRLIGPNCMGIYYPKNGMAFFPGMPKEHGKVGM
                     ISQSGSLAILLSRLAGPKGVHFSKVISSGNESDLNSSDFLFFLGEDPETEVVGAYLEG
                     VKDGRAFLRALQAVCRKKPVVIWKAGRTPKGTRAASSHTGSLAGSRMAWEALKEQTGA
                     ILVRDLEEFIDILTAFYYLPRKVGRRLAILSGPGGPAVSAADACEEFGLEVADLQEET
                     RRRLKAMLPQTGTSADNPVDLGLASVFQVELYGRASEIVGEDPGVDGLLLQGRGATAE
                     LDAQYARLVTEARKKVRKPFMAISLGGMYLEEESRKALLEAGIPVYSSAERALWAYSR
                     LYQYGERHLEG"
BASE COUNT          544 a          704 c          619 g          508 t
ORIGIN      
        1 agtaaccagg aggccgatcc aaccggcaaa ccccactgga tggatgatca catcgtaccc
       61 cggaggtatt tccccgacta cccaccgact aattaaccaa agaaggaggg aatcccccaa
      121 attggtcccc acctgtcctt cgattttctt aacctccgag aggctcagcc cgagaatgag
      181 aatggggacg cagaccataa ctcccactag cgggccggcg gcgccgatgt ccatgagcat
      241 ccgacggtct cttgccgggg accgcatttt aatgaaggcg ccgaaggtcc cgatgaaaga
      301 tggggcagga atgaaataag gcagggttac gttcacccca tttttgcggg ccaccaggta
      361 gtgacccaat tcatgggcca gaagaatccc catcaaagcg acggcaaagg ggattccccg
      421 gataatctgc tcggggtggg taagcggatt caccccttcc tgcaaagccc cggccaagac
      481 cgtggtgatc agggtgaggc aaaacaacac cagattccag aaagccctgg gtttgccggg
      541 tggagtcgga taaggcaagt cctctccggc ccgggttaaa atcgcttttt ttcgccaagg
      601 ccaaaaatcc ttgaagttca tgtcgtctcg taagaattcg ctctttctga ctcgaatcct
      661 ttctgtagaa aagggcgaaa taagggggca gcctttttca ttccttcacc ctgcaagagc
      721 ttctttccgt attttccttc tggtcttttc cgaaatcccc atcatccttc gagatgtctc
      781 tctccgtact gataaagacg ggaataggcc caaagggccc gttcggccga ggagtaaacg
      841 ggaatcccag cttccagaag ggccttcctg gattcttctt ccaggtacat tccccccagg
      901 gagatggcca tgaaaggttt gcgaaccttc ttacgcgcct cggtgaccag gcgggcatac
      961 tgagcatcta gctcggcagt tgctccgcgg ccctgaagga ggaggccatc cactccaggg
     1021 tcttctccca caatttccga agcccgccca tagagttcta cttggaagac cgaagccagt
     1081 cccaggtcca cgggattgtc cgcgctggtc ccggtttgcg gtagcatggc tttcaaccgc
     1141 ctgcgagtct cctcctgcaa gtcggccacc tcgaggccga attcttcaca ggcatcggca
     1201 gcggatacag caggtccacc cggtccggat aaaatggcta gacgccttcc cactttccga
     1261 ggcagataat agaatgcagt aagaatatct atgaactcct ccaagtcacg gacgaggatg
     1321 gctcctgtct gctctttcaa ggcctcccag gccattctgg acccagccag ggacccggtg
     1381 tgggaagaag cggctctggt ccctttagga gtccgccctg ctttccagat gaccaccggc
     1441 tttttccggc agaccgcctg cagagcccgg aggaatgcgc gcccgtcttt caccccttcc
     1501 aggtacgccc cgaccacttc tgtttccggg tcttccccca ggaaaaaaag aaaatcggag
     1561 ctgttcagat cgctctcatt tccgctgctg atgaccttgc tgaaatggac tcccttgggc
     1621 ccggccaatc ggctcaatag aatggccaga gagccacttt gggaaatcat ccccaccttc
     1681 ccgtgctcct tgggcatgcc cgggaaaaaa gccatgccgt tcttggggta gtagattccc
     1741 atgcagttag gcccgatgag ccgtatccct tccttgcggg caacctgcag aagcgcggcc
     1801 tccctttttt tcccctcttc atccccggtc tcagcgaatc cggaggtgta gatgaccgcg
     1861 attttcactc ctttttggcc gcattcctcg agtgccggca aaaccagggc ggctggcaac
     1921 aggaggatag ccaggtcgat ctctcccgga acttccttca gggaggggta ggctcggcat
     1981 ccatcgatgg aatccgcctt ggggttgatg ggaaagacct ttccggaata gccctgttca
     2041 agaagcccct ggagaaaaac ccttcccggc tcaaagccgc gggggacgcc taccaaagcg
     2101 atagatcgag ggttgaaaag gaaacctagg tcccttctgc gattcaagct gcgctccttt
     2161 ccgcgggaaa aaaccccccg gggggaggaa aaatttaacg agaaccgcac ccatcttcct
     2221 ccttaaaaat aacaaaagct ccccttcgag gcaactgaaa ttcctatttt ttcttgccct
     2281 ggagacggga gagcctgggg aaaaggagtc ggagaaaacg agggcctact tgaaagaccc
     2341 tgcctcccta tcatagggaa tcttatgtag gaatc
//
LOCUS       DASPRR010000200         9356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1984633, whole genome shotgun sequence.
ACCESSION   DASPRR010000200 DASPRR010000000
VERSION     DASPRR010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984633"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1591)
                     /locus_tag="VEL68_07780"
     CDS             complement(143..1591)
                     /locus_tag="VEL68_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ35902.1"
                     /translation="MEKREKHFLEKFLSPESVAIVGASRNPMRPSHNLVANQVRLGFQ
                     GKVYPVNPETNEIAGVKTYPDLKSIKGPIDLAVIAVPYNLAPNLLQEALARGIKRITV
                     VAGGFSETGEDGKRVQKEMALFLRQNGARAIGPNALSPINAKVNFAISFFPLQKLKAG
                     GVSFIFQSGLYEPRLDWLFSDFNLHLCKLIDLGNKMDVSEVDVLSYLSQDPNTRVIAI
                     HLESIEGKGREFLRLIREASRYKHVVVLKSGRTEAGAQMAASHTGVIVRGNDLVFDAA
                     LKQAGAIRAQNIEDFFDLARALERFGPYRFKGTRLAVATLPGGEAVVVTDLCHQAGFS
                     LPRLHENTLEKLRAVFPPWDISGNPFDLGVTLQFHDPRKVYYTLLKAVTEDPNVDAVA
                     IQLPPRLLNVPREFFHPFVQALNAQKPIALWVAGFPSGRDETLQWVEDQHVPVFSSPE
                     RALNALFALQRSSLWKSHVPPAEPGAVLREAE"
     gene            1959..3449
                     /locus_tag="VEL68_07785"
     CDS             1959..3449
                     /locus_tag="VEL68_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXZ35903.1"
                     /translation="MEEKEAARINPEEEEKKIDAEEEGSFAELFEKSSQTAYGRFSPG
                     DRVTGKIVKVSADTVFIDLGGKSEGTANAVEFKDQDGTLTIQEGDEVELRVASVRGGI
                     HLSKAIKARGAEAVEILRDAFRNQIPVEGRVAAVNKGGFEVEISGLRAFCPISQMELR
                     YCEKPEEHVGARYQFRIMEIKERGKNIVVSRRMLLQEEQERKLQETLAALRPDLELEG
                     QVTKLTDFGAFVDIGGVEGMVHVSEVSRARIGHPSEILQPGQTVRVKILKMEPDKKGR
                     QKISLSMKALEPDPWEKGIPFQEGDVISGKVSRLADFGAFVEIIPGLDGLVHRTEISY
                     EKVSHPSRVLNVGDPVQARVLKIDEPNRKISLSIKAAMVFGQNGEGEEDVRLEVGQVL
                     KGIVEDQKPYGLFVRLPQLGMKTRGLLPLEELLEADRSDVKKKMLPGKEVQVEIIAIE
                     EDNKIRLSQKSIKEREDRGDYEKFLKKPGRGSSLGTLGELFQKLKK"
     gene            complement(3499..4779)
                     /locus_tag="VEL68_07790"
     CDS             complement(3499..4779)
                     /locus_tag="VEL68_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35904.1"
                     /translation="MDKKTNFFFYGWYIALVGATSYALGYGARFSFSVIFPSLLEEFK
                     WSRDHTATMFSVHLLTYGIAAPITGFLVDRRGPRKTMVLGAILLALGLVLSRWGNQLW
                     YFWITFGLISGGGLCMIGAVPFTTVLRNWFEKRRGLAFSLLFLGSGGAYGCYPAIAWL
                     IDTVGWRNTYLIEGLVVGGVLIPLILFVVHYHPRDIGLFRDGLAEWQEEPTSSGKGVL
                     RVVDPAWAAVSWTLSKAVRTGRFWLLCLTTFCLWGINQNILLAHQVAFAMDMGFSKLY
                     ASSVLSLFGLTYAFGCLASLISDRIGREVTITLATAIGISGLIVLMFIRDSSQPWMLY
                     YYSISLGAANGLCSPTIAASITDIFQGPRVGAIVGAVWFAFALGGTIGPWLGGWLFEL
                     TGNYFLAFVVATVLFSVACAAIWLAAPRKVRRVS"
     gene            5132..6154
                     /locus_tag="VEL68_07795"
     CDS             5132..6154
                     /locus_tag="VEL68_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ35905.1"
                     /translation="MKGIYLTGPNNFSLQDLNDPVPGKNDVLIKVRMAGICGSDVHLL
                     RGRNPFAAYPLVPGHEYMGEVLQAPKGRGLRKGERVTVFPEVGCGKCPACKEGRLIHC
                     PEFKFVGVRVPEGCFCEKVAVPVERVIKLPKTMRDEEGAMVEPTAVGVHAVQRGGVKR
                     GDRVVVIGGGTIGLLTAQAARAFGASKVVVSEPIAERRKIAQTLGFKLICNPMEHDLL
                     AFIKDEIGLVDVVFDVVGFKKTLEDSQAMLRPDGTLVLVALPHIEGLGVPYQPIFAKE
                     LRVIGSRTYFMKDFPLAIRLMQAKKIRVKPIISEIIPLARFNEGLDHLEREPEKYVKV
                     LVRPTE"
     gene            complement(6282..6446)
                     /locus_tag="VEL68_07800"
     CDS             complement(6282..6446)
                     /locus_tag="VEL68_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35906.1"
                     /translation="MTRIMAKAAARLLKELKEKGSTRGMLNHMFSHSGLFELFDFLQW
                     VELEKRFLKI"
     gene            complement(6457..7584)
                     /locus_tag="VEL68_07805"
     CDS             complement(6457..7584)
                     /locus_tag="VEL68_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35907.1"
                     /translation="MGHFINDLYPAFLPPLLPLLVEKFQLTFTRAGILATILSFSASM
                     TQPLFGYLADRLGGRTLILLGPLVCGVSLSFIGVVHHYPLLIFLLIFGGMGGAAFHPE
                     AAAMAASLSGGKKTLGMSVFMLGGNAGYGLGPFLILLTVSNLGMDWSFLSSLPALGLV
                     WLLYRYPPAEEVSISPCPGVRPPVERRMNRRLSSFGLLLGVVVLRVTAVLSLTTFLPM
                     IQRLRGFSLVAAGSSFTVFMVCGALGGLTGGVLADRVGRKKIIVTSSILILPALLAFL
                     VWKGLASFLILSLLGFLFFLSESACIVLAQEMSPGKARTASGIIMGMAWGLAGFGVLG
                     TGALADALGIEWAMRFLLLLPAGSLFFSLFLPGAQGQRMIK"
     gene            7840..8544
                     /gene="pxpB"
                     /locus_tag="VEL68_07810"
     CDS             7840..8544
                     /gene="pxpB"
                     /locus_tag="VEL68_07810"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HXZ35908.1"
                     /translation="MRDWPRIKPSGDSALLVEFENRIDPEVNRSVRALALTIESDRPP
                     GVQEILPAYRTLMVTYDPLQIEYPLLAEKIQGWTNKAERIDLPPKRLFRLPAVYGGAH
                     GPDLEHVAKVLDLTPGEVIRIFSQTRFPVYFIGFICGLAYLGGMPEKLRVPRLASPRT
                     LVPAGSVGFAGGQANAIATDQPSGFNYIGRTYVILYDPKLFPPIPFVAGDEVSFPPVS
                     EEEALRVKGRGAEDFL"
     gene            8541..>9356
                     /locus_tag="VEL68_07815"
     CDS             8541..>9356
                     /locus_tag="VEL68_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HXZ35909.1"
                     /translation="MKALEVINPGVYTILQDLGRPGYMKYGIPASGAADRFSSQVANL
                     LVGNKPEATVLETTLFRLELLALENLAMAVTGGDLSPTVNKNPFPMWQAVSIRKGDRI
                     SFRGRKRGFRAFLAVRGGFSGPRYLGSRSVFSRGLMGNSLRPGEILEIEDSTVFPSFD
                     ATLPVNLIPDFSLRPTLRVILGPQQDRFSPKGLETFLSSEYTVSPQSDRMGYRLEGPK
                     IQHVQGADIISEAIARGAIQVPGDGLLIIMLWDAQVSGGYTKIANVITADHDLL"
BASE COUNT         2228 a         2515 c         2730 g         1883 t
ORIGIN      
        1 accgtaatcc tcaaagcagc cttcggctta aaagtgaggt gtaatggggg caggagaggc
       61 aaagacttga tttggcagtg ttccggtaaa gtcaaatata tgttcttata tctccctccg
      121 tctagaatct ccgaattttg cttcattccg cttcccgaag gactgcccca ggttctgccg
      181 gagggacatg acttttccac aaacttgaac gttgcagagc gaacagagcg ttcagcgctc
      241 tttccggcga agagaagacg ggaacatgct ggtcctccac ccactggagc gtttcgtccc
      301 ttcccgaggg aaatccggcg acccagagag caatcggctt ttgggcattc agggcctgga
      361 cgaagggatg gaaaaattct ctaggaacgt tcaagagccg ggggggaagc tggatggcca
      421 cggcgtccac attcgggtct tccgtcacgg ccttgagaag tgtgtagtaa accttccggg
      481 ggtcgtggaa ctgcagggtg actccgaggt cgaagggatt gccggaaatg tcccagggag
      541 gaaaaacggc cctcagcttc tccagggtat tctcatggag acggggcagg gaaaaaccgg
      601 cctggtggca gaggtcggtt accaccacag cctcccctcc gggtaatgtg gccactgcca
      661 gccgagtgcc tttgaatcga tatgggccga agcgctccag ggctcgggcc agatcgaaga
      721 aatcttcgat gttttgagcc cggatggcgc cggcctgctt caaggcggcg tcgaacacca
      781 ggtcgtttcc gcggacgatc accccggtgt gggaagccgc catttgcgct cctgcttccg
      841 tgcgaccgga tttgagcacc accacgtgct tgtaccgcga tgcttcccgg atgagtctga
      901 gaaattctct cccctttccc tcgatgctct ccaggtgaat ggcgatcacc ctggtgttgg
      961 ggtcctgaga cagataggaa agcacatcga cctcgctgac gtccatttta tttcccaggt
     1021 cgatgagctt gcagaggtgg agattaaaat ccgagaagag ccagtccaga cggggttcat
     1081 aaagacccga ttggaaaata aaagagactc cgcccgcttt caacttctgc aggggaaaaa
     1141 agctgatcgc aaaattcacc ttggcgttga tcgggctcag ggcgttcggg ccgatggccc
     1201 gggccccgtt ttgcctgagg aagagagcca tctctttctg gacccttttc ccatcttctc
     1261 ctgtttcgga aaagccccca gccacaaccg taattcgttt gattcccctg gccagggcct
     1321 cctgcaaaag attgggggcg aggttgtaag gaacggcgat caccgccaga tcgatggggc
     1381 ctttgatgct cttcaagtcc ggataggtct tcaccccagc gatctcattg gtttccgggt
     1441 tcaccggata aaccttcccc tgaaacccga gccgtacctg attggccacc aagttgtggc
     1501 tcggccgcat cggattccgg gaggccccta caatggccac cgactcgggg gaaagaaact
     1561 tctccaaaaa atgtttttct ctcttttcca tggatccctt tcgtcaattc cgaattggga
     1621 gcattcccct tgatgttttc gccccgccct ctttattatt aaatccaagg ggaaaatgca
     1681 agcccttctg gattgcggat tgcggatttc agattgcgga ttgtaaacaa aggcgattct
     1741 ttttgaatgg ggaatgcgcc ctgaagcggg tacgattggg ggctggtccg cgctcaacgc
     1801 ggtgcagggc gcacgcgaga atattcccgg aaaaaccgaa aaacgttgag tttccttctt
     1861 cttatggtat gctaatccag gggatgtgag ggcagaatca ggctggcatt accgtcgtgc
     1921 cccacgctga catcaacaat tctagtgagg aaccggggat ggaggagaag gaagcagcga
     1981 gaatcaatcc ggaagaggaa gaaaaaaaga tagatgccga ggaagagggc agttttgccg
     2041 agcttttcga aaaaagttcc cagaccgcct acggccgttt ttcgccaggg gaccgggtca
     2101 cgggaaaaat cgtaaaggtc agcgcggata cagtttttat cgatctgggg gggaagagcg
     2161 aagggactgc caatgccgtt gaattcaagg accaggacgg taccctgacc attcaggaag
     2221 gggatgaagt cgagctgcgg gtggcttcgg tccgaggggg gatccacctg agcaaggcga
     2281 tcaaagctcg cggggccgag gccgtggaga tccttcggga cgctttcagg aaccagatcc
     2341 cagtggaagg aagggtggct gcggtcaaca agggaggttt cgaggtggag atctccgggc
     2401 tgcgggcctt ctgccccatc agccagatgg aactccggta ctgtgaaaag cccgaggagc
     2461 atgtaggggc ccggtaccag ttccggatca tggagatcaa ggagagaggg aagaacatcg
     2521 tcgtttcccg acgcatgctt ctgcaggaag aacaggaaag aaaactccag gaaaccctgg
     2581 ccgccctcag gccggacctc gagctcgaag gccaagtgac caagctaacc gacttcggcg
     2641 ccttcgtgga tatcgggggg gtcgaaggga tggtccatgt gtccgaggtc tcccgggcgc
     2701 gcatcgggca tccgtcggaa atcctccagc ccggtcaaac ggtgagagtg aaaattctca
     2761 agatggagcc ggataaaaaa ggccgccaga agatttctct ttccatgaaa gccctggaac
     2821 cagacccctg ggagaagggg attcccttcc aggaagggga tgtgatttcc ggaaaggtgt
     2881 cccgcctggc cgatttcggc gcttttgtgg aaatcatccc gggattggac gggctggtgc
     2941 acaggactga gatcagctat gaaaaagtct cccaccccag ccgggtgttg aacgtcggag
     3001 acccggttca ggcccgggtc ctgaagatcg atgagcccaa tcggaagatt tccttgtcta
     3061 tcaaggcggc catggtattt ggacagaatg gagaggggga ggaggatgtt cgtctcgagg
     3121 tgggacaggt cttgaagggg atcgtggaag accagaaacc gtacgggctc ttcgtaaggc
     3181 ttccccagct cgggatgaag acgcggggac tgctccccct ggaagaactc ctcgaagccg
     3241 accggtcgga tgtgaaaaaa aagatgctcc cggggaaaga ggtgcaagtg gagatcatcg
     3301 ccatcgaaga ggacaacaaa atccgccttt cccagaagtc catcaaagag agagaggacc
     3361 ggggcgatta cgagaaattc ctgaagaaac cgggccgggg gtcctcgctc gggacgctcg
     3421 gagagctttt ccagaagctg aaaaaataaa agacccatcg caagtgcggg gaagatgccg
     3481 cacgtttttc ccgctccctc aggaaacccg tctcaccttt cgcggcgcgg ccagccagat
     3541 ggccgcgcag gccacggaaa aaagaaccgt cgccacgacg aaagccagga aataattccc
     3601 ggtcaattca aacagccatc ccccgagcca ggggcctatg gttcccccca gggcgaaggc
     3661 gaaccatacc gctccgacga ttgccccgac cctcggaccc tggaagatgt cggtaatgga
     3721 ggcggcgatg gtgggtgaac agaggccgtt ggcggccccc agcgagatgg aataatagta
     3781 gagcatccag ggctgggaag aatctcgaat gaacatcagg acgatcagcc cggagatccc
     3841 gatggccgtg gccagggtga tggtcacttc ccgcccgatc cggtcggaga tcaggcttgc
     3901 caggcacccg aaggcgtagg tgagtccgaa caaggaaagg acggaggagg cgtaaagctt
     3961 ggaaaacccc atgtccatgg caaaagccac ctgatgggcc aggagaatgt tctgattgat
     4021 tccccagaga cagaaagtcg taagacagag cagccagaag cggccggttc gtacggcttt
     4081 tgaaagagtc caactcacgg ccgcccatgc cgggtccacc acccgcaaca cgccttttcc
     4141 cgaggaggtg ggttcttcct gccattccgc aagcccgtcg cggaaaagtc ctatgtcgcg
     4201 ggggtggtag tggacgacga ataggatgag gggaatcaga accccgccca ccaccaggcc
     4261 ctcgatcagg taggtgttgc gccatcccac ggtgtcgatc agccaggcga tggccgggta
     4321 gcagccgtag gcaccccccg aacccaggaa gagcagggaa aaggccagcc ccctcctttt
     4381 ttcgaaccag ttgcggagga ccgtggtaaa gggaaccgca ccaatcatgc agaggccgcc
     4441 cccggatatc aggccgaagg tgatccagaa ataccagagt tggtttcccc aacgggacag
     4501 gacgagtccc agggccagaa ggatagcgcc caggaccatc gtcttccttg gccccctgcg
     4561 gtccaccagg aacccggtga tgggagccgc aattccgtaa gtaaggaggt gcacggaaaa
     4621 catggttgcg gtgtggtccc gggaccattt aaattcttcc agcagggaag ggaagatgac
     4681 cgaaaaggaa aaacgggccc cgtaccccag ggcgtaagag gtggccccga cgagggcaat
     4741 gtaccatcca taaaagaaaa aattggtctt cttatccatc tgcggagttt gccatctgga
     4801 atcttcaaag gataacggca gacaggcgta gaataccacg attaagccct tgaaaccaat
     4861 tatctttcgc tatctggacc aaaaacctcg ccccctttaa atcataaaga aagcagtctc
     4921 ctctgtcctt tagcgctacg ccttaagccc tctgctccca gcttttttag gggtgaatgg
     4981 cgagtttcct tgtaaattgc caaaaattca gtgtattata tcgggcaatc gagccgcggg
     5041 gagaattcta agtaccctgg atgaattgag ctcggcttga aggaggagag cgggttaaag
     5101 gttggttgca aactcaagag gggagggcgc gatgaaggga atttatctca ccggtccgaa
     5161 caatttctcg ctccaggatt tgaatgatcc ggtacccggg aagaatgatg tgctcataaa
     5221 ggtccgcatg gccgggatat gcggctcgga tgtccactta ttgaggggac ggaacccttt
     5281 tgccgcctat cccctcgtgc cggggcatga gtatatggga gaggtcctcc aggctcccaa
     5341 aggccgcggc ctgagaaaag gggaaagggt aaccgttttt ccggaggtgg gttgcggcaa
     5401 atgcccggcc tgcaaagagg gaaggctgat ccattgcccc gaattcaaat ttgtgggggt
     5461 aagagtaccg gaaggatgtt tctgcgaaaa ggtggcggtt cccgttgaac gagtcatcaa
     5521 gttacccaag accatgagag atgaagaggg ggccatggtg gagccgacgg cagtgggcgt
     5581 tcatgccgtc cagaggggcg gcgtgaaaag aggagacagg gtcgtggtca tcgggggagg
     5641 gaccatcggc ctgctcacgg cccaagcggc ccgggccttc ggggcctcca aggtggtggt
     5701 ctccgagccc attgcggaga gaaggaagat cgctcagacc ctgggcttta aactcatctg
     5761 caatcccatg gaacacgacc tcctggcctt catcaaggat gaaataggtc tcgtggatgt
     5821 ggtattcgat gtagtgggat tcaagaagac gctggaagac agccaggcca tgcttcgtcc
     5881 tgatggaacc ctcgtcctgg tcgccctgcc ccacattgaa ggactgggag taccgtacca
     5941 gcccatcttt gcaaaggagc tccgggtcat cggttcccgg acctacttca tgaaggactt
     6001 tcccctggcc atccgcctca tgcaggccaa gaaaatccgg gtgaagccca tcatcagtga
     6061 aatcatccct ttggcgcgtt tcaatgaagg cctggatcac ctggaaagag aacccgaaaa
     6121 atacgtgaaa gtcctcgtcc gtccaaccga ataggtcaca tccttgcttt tggcggttcc
     6181 cgaaacgctc ctcctctccc gggaggcccg caggttcttt tccttttgac ttcttgttag
     6241 gtctgtttcc gcctcctcct tccattcctt gtctgaaggc tctatatttt tagaaaacgc
     6301 ttctccaatt ccacccactg gaggaaatcg aagagttcga aaagcccgct atgggagaac
     6361 atgtggttca gcatcccccg agtgctccct ttttccttga gctctttcaa gagcctggcc
     6421 gccgccttgg ccatgatccg agtcaaggcg ttgatttcat tttatcattc tttgaccctg
     6481 agctcctgga aggaaaagag agaaaaacag agacccggcg ggtagaagga gaaggaatcg
     6541 catcgcccat tcaattccca gggcgtcggc cagagcccca gtccccaaaa ctccaaaacc
     6601 agccaggccc caggccattc ccatgatgat ccccgaagcg gttcgggctt ttcctgggga
     6661 catctcctgg gccaaaacga tgcaggccga ctcgctaagg aaaaacaaaa accccaagag
     6721 tgacaggatc agaaaactgg ccagtccttt ccacaccagg aatgccagaa gcgcgggaag
     6781 gatgaggatg gatgaagtga caatgatctt cttccttcct acccggtccg cgaggactcc
     6841 tccggttagg ccccctaaag cgccgcagac catgaagacg gtaaaggagc ttcccgcagc
     6901 cacgagagag aagccccgca gtctttgaat catggggaga aaagtggtta gactcaaaac
     6961 ggccgtgacc cgaaggacaa caactcccaa taggaggcca aagcttgaaa gcctccgatt
     7021 catacggcgt tctacgggtg gacgaacccc agggcaagga gaaatagata cttcttccgc
     7081 agggggatag cggtatagaa gccagaccaa tcccaaggcc ggtaggctag agaggaagct
     7141 ccagtccatt cccaggttgg aaacggtcag cagaatcagg aaagggccca ggccataccc
     7201 tgcgtttcct cccagcatga agacggacat ccccaaggtc ttcttgcctc cgctcaggga
     7261 agcagccatg gccgcggctt cggggtgaaa agcggctcct cccattcccc cgaagatcaa
     7321 taggaagatc aataaaggat aatgatggac aaccccgatg aaactcaggc tcaccccgca
     7381 aaccaggggg cccaggagga tcaaagttcg acccccgagt ctatcggcta ggtacccgaa
     7441 caggggctgg gtcatggaag ctgaaaagct gagaatcgtg gccaggatgc cggcccgggt
     7501 gaaggtgagc tggaattttt ccaccaggag ggggagaagg gggggaagaa aagccggata
     7561 gagatcgttg ataaaatgtc ccaaagcaag gagcgccgcc ttagggaaag atgttcttga
     7621 atcttttccg tccatgcctt tttatgatgg tctctccgca gcgaaaagga aagaaaaaat
     7681 tttacagggt cccagggggg atggtatagt tttcaaaaac ggccggcgtt tgccggttac
     7741 tcgttgcccg tttcccatga ccggtttcca ttgagcggat tttgacgacg gataacaaac
     7801 tgccgacaag gacaattgac ttccaggcga ggaacctgaa tgagggattg gccccgaatt
     7861 aaaccctccg gcgacagtgc cctgctggtt gagttcgaaa accgaatcga cccggaggtg
     7921 aaccgttcgg tgcgggctct ggccctgacc attgaatccg atcgtccgcc aggagttcag
     7981 gaaatcctgc cggcctaccg gacgcttatg gtgacctacg atcccttgca gatcgaatat
     8041 ccccttttgg cggaaaagat tcaggggtgg acgaataaag cggaacgtat cgaccttcct
     8101 cccaagcgcc ttttccgact ccccgccgtg tacggagggg ctcacgggcc ggacctggag
     8161 cacgtagcga aggtcctcga tcttacccct ggagaagtga tccggatttt ttcgcagacc
     8221 cgtttccccg tttattttat cggtttcatt tgcggcctgg cttacctggg aggaatgccc
     8281 gaaaaactcc gcgtacccag gctggccagt cctcgaacgc tggtaccggc aggttcggtc
     8341 ggatttgccg gagggcaagc caacgccatc gctacggatc aaccctcggg gttcaactac
     8401 atcgggagga cctacgtgat cctttacgat cccaagctct ttcctcccat cccctttgtc
     8461 gctggggatg aggtttcctt cccgccggtg tcggaagaag aggccttaag ggtgaaaggg
     8521 agaggggcgg aggattttct atgaaagccc ttgaggtaat caaccccggg gtttatacga
     8581 tccttcaaga cctgggccgg ccgggttaca tgaaatacgg gattcctgcc tccggtgcgg
     8641 ccgaccgttt ttcctcccag gtggcgaacc tcttggtcgg gaacaagccg gaagccactg
     8701 tcctggaaac gaccctcttc cgcctggagc ttctggccct ggagaacctt gccatggcgg
     8761 tcaccggagg agacctgtca ccgacggtga ataaaaaccc ctttcccatg tggcaggcgg
     8821 tttcgattag aaaaggcgac cgaatctcct ttcgaggccg gaagagagga ttcagggcct
     8881 ttttggccgt ccgaggaggg ttttccgggc cccggtatct gggcagccgg tcggtctttt
     8941 cccgtggcct gatgggaaat tccttaaggc caggagaaat cttggagatc gaagattcga
     9001 cggtattccc ctcctttgat gccacgcttc cggtgaatct catccctgac ttttctctcc
     9061 gtcccaccct gcgagtgatt cttggacccc aacaagaccg gttctctccg aaaggcctgg
     9121 agacttttct ctcctccgaa tacaccgtaa gcccccaatc agaccggatg ggatatcgct
     9181 tggagggacc caaaatccaa catgtccagg gagcagacat catttccgag gccattgctc
     9241 ggggggccat tcaagttcct ggggacggcc tgctgatcat catgctttgg gatgcccaag
     9301 tttcaggcgg gtataccaag atcgccaatg tgatcactgc cgatcacgac cttctt
//
LOCUS       DASPRR010000201         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1660841, whole genome shotgun sequence.
ACCESSION   DASPRR010000201 DASPRR010000000
VERSION     DASPRR010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660841"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..462)
                     /locus_tag="VEL68_07820"
     CDS             complement(244..462)
                     /locus_tag="VEL68_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35910.1"
                     /translation="MRRIVVVRHLIRGSETLKIKERLGMVPNVGSDQGRSKITDAPSS
                     WELRVSCGILSRVRKSKKFKSLQGALRP"
     gene            612..1976
                     /locus_tag="VEL68_07825"
     CDS             612..1976
                     /locus_tag="VEL68_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXZ35911.1"
                     /translation="MVQKTNWHYFIGAGWILFFFVLHFPGISRAGEGFPDYEKQYLSY
                     AGKTEANQLDTEEPPGNVTLQTAIAMALLKNPELQSFSLEVRAREARMIQARLLPNPE
                     FQLGVENVFGSGALRGFDGAESTFSLAQPFLLGGKRDKRGRLANADRDLAKWDYEARR
                     FDVLSEVNKAFVDTLAKQERVTLAEDLVHLAEQSFDVVRSRVQAGKVTPIDETKASAA
                     LSLVRIELLRARRELESARKILAATWGSRAPVFSRVEGRLGLDAEVPPFEQLTRNLDR
                     NPDIARWDSEMDQRRATLTLEEANRIPDIALSGGVRRFSATDDNAFVFGFSVPLPVFN
                     RNQGAIAEAKNRILKVEKERQAIFLRVQAGAAEAYQRLASAFEEARTLQNQVLPSLQE
                     VFDSVREGYFYGKFGYLDLLDSQRTLFESRGRYLDALANYEKAATDVERLTGKSPRRP
                     SSPR"
     gene            1988..3232
                     /locus_tag="VEL68_07830"
     CDS             1988..3232
                     /locus_tag="VEL68_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ35912.1"
                     /translation="MGRRNLAFMVIGLAIGILATIAFFHWYPFHPLKAKEGSGAVPRH
                     EKDGQHAGEKEGEKRSEARVVKLTEAEKREFGVEVRQAGPGKLIERLSLPGEIQLNPD
                     RVGQIVPRVPGVIRSVTKSAGDSVRAGELMAVLESRELADSKAAYLAALKRVELAEAT
                     LKREEMLWRKKISPEQDYLEARNSLAEARINLNSADQKLRALGFSEDHLRQLPSQNDA
                     VYTTYQIRAPFDGTVIERHLNPGEFHKEDAPAFRVADLSTVWVKISIYQKDLPLVRKG
                     QRVHISAGRGIPDTEGQIAYIEASIKEQTRTAIAVVILPNPQGLFRPGLFVSCSLEAE
                     EIPVPLLVPKTAVVQRDAQAVIFLETEDGFEPRPVAVGRSDETHVEVVSGIKAGQRYV
                     SKGGFTLQAQLSRHGFGDGHAH"
BASE COUNT          768 a          907 c          946 g          769 t
ORIGIN      
        1 atcatcccta gccccgggtc tggaggatcc tcgctagggg ctttcacttt tccgtttgac
       61 ccgataagaa ggggatagta taatttcccc atctatgaaa tggttgaaga gtaaaagtgg
      121 tttcttttgg ctcttttttt gctactctgt tctctccgcg gtttccccac tccaggttct
      181 cgaaatatgt ttcgaagcag aaggccattt aacgataggc ttcggccaaa cttgtgaaaa
      241 acatcaaggt ctcagtgctc cctgtaaaga tttgaacttt ttagacttcc taactctgga
      301 taaaatcccg caggaaaccc tcagttccca tgatgatggc gcatctgtaa ttttgcttct
      361 tccctggtcg gagccaacgt ttggaaccat cccaagtcgt tcttttattt tcaatgtttc
      421 tgaccccctg ataagatgtc tgacaaccac gattcttctc atctgatgac gttctttcct
      481 tccaaataac tttccttcat cattcgggca cattcatttt cgaaacaacc ctcttcgtcc
      541 tgcatgttaa aggaaaacaa agacccttta aggtgggggt gaacttttat tagatggatt
      601 tcggaggcca aatggttcag aagacaaatt ggcactattt tattggtgca ggctggattc
      661 tgtttttctt cgtcctccat ttcccgggaa tatcccgggc gggagaaggt ttccccgact
      721 atgagaaaca atatctttct tatgcaggaa aaactgaggc gaatcaactc gacacggaag
      781 agccgcccgg aaacgtcacg ctccaaaccg caatcgcgat ggctcttttg aaaaatccgg
      841 aattacagtc tttttccctg gaggtccgcg cccgtgaggc gaggatgatt caggctaggt
      901 tgctgcccaa tccggaattt caactggggg ttgagaatgt cttcgggtct ggagccctgc
      961 ggggatttga cggggcggaa agcacattct ccttggctca gccttttctg ctggggggaa
     1021 agagggacaa acgaggtcgc ctggccaacg cggaccggga cttggcgaaa tgggattatg
     1081 aggcccggcg gttcgatgtc ttatccgagg tgaataaggc cttcgtggat accctagcca
     1141 aacaggaaag ggtcaccctg gcggaagatc tggttcattt ggccgagcaa tcctttgacg
     1201 tggtccggtc ccgggttcaa gccggaaaag tcacgcccat cgatgaaacc aaggcctcgg
     1261 cggccttgtc cctggtgcgc attgagctgc tgcgggcccg gcgcgaactt gaatcggccc
     1321 ggaaaatcct ggctgccacc tggggcagcc gggcgcccgt tttttctcgc gtggaaggac
     1381 ggctgggatt ggacgctgag gtgcccccgt tcgaacaact tacccgcaat ctcgaccgaa
     1441 atccggatat cgcccggtgg gattcggaga tggaccagcg ccgcgctact ttgaccctgg
     1501 aggaggcaaa ccgcatcccg gacatcgcct tgagcggggg ggtcaggaga tttagtgcga
     1561 cagatgataa cgccttcgtc ttcggctttt ccgttcccct cccagtattc aatcgaaatc
     1621 aaggggccat cgcggaagcc aagaaccgga ttttgaaggt ggaaaaagaa cggcaggcca
     1681 ttttcctgcg agtgcaagcc ggggccgccg aagcgtacca gaggttggcc agcgcctttg
     1741 aggaagccag gaccttgcag aaccaggttc ttccctccct gcaggaggtc tttgattccg
     1801 tgagagaagg gtatttttac ggtaaattcg gctacctgga tcttctcgat tcccaaagga
     1861 ctctttttga atccagaggg cgttaccttg atgccctggc caactatgag aaagctgcta
     1921 ccgatgtgga gcggctgacc gggaaaagcc cccgtaggcc atccagcccg agataaggga
     1981 gaaaaatatg ggaagaagga atttggcctt tatggtgatc ggtttggcga tcgggattct
     2041 ggcgacaatc gctttctttc actggtatcc tttccacccc ctcaaggcaa aagaggggtc
     2101 cggagcggtg cctcggcacg aaaaggatgg gcaacatgcc ggagaaaaag agggcgaaaa
     2161 acgttccgag gcaagagtcg taaagctcac cgaagcagaa aagcgcgaat tcggcgtcga
     2221 ggttcgccaa gccggacccg ggaagctgat cgaaagactc agtctgcctg gggagatcca
     2281 gctgaatcct gaccgggtcg gccaaatcgt accccgtgtg cccggcgtca tccgcagtgt
     2341 caccaaaagt gcgggggact cggttcgcgc gggcgaactg atggccgtct tggaaagccg
     2401 tgagcttgcc gacagcaagg ccgcctacct tgcggcgttg aagcgcgtcg aactggccga
     2461 ggccaccctg aaacgggaag agatgctttg gcgcaaaaag atctcgccgg agcaggatta
     2521 ccttgaggcc cgtaactccc tggccgaggc acggataaat ctaaattcgg cggatcagaa
     2581 actccgggcc cttgggtttt cagaggacca cctcaggcaa ttgccttcgc agaacgatgc
     2641 cgtctacacc acttaccaaa tccgcgcacc cttcgacggc acggtcattg aaagacacct
     2701 caatccgggg gaatttcata aggaagatgc gcccgcgttc cgcgtggccg atcttagcac
     2761 cgtctgggta aagatcagca tttaccagaa agaccttccc ctggttcgca aagggcagag
     2821 ggttcacatc tccgcagggc gggggatacc ggacactgaa ggccagatcg cctatatcga
     2881 agcgagcata aaggagcaga ctcgcacggc aatcgccgtg gtcattctac ccaatcccca
     2941 gggcctgttc cgccccggtc tcttcgtttc ttgcagcctg gaggctgaag agatcccggt
     3001 tccccttctc gttccgaaaa cggccgtggt tcaaagggat gcccaagccg taatcttcct
     3061 tgagaccgag gacgggttcg aaccccgacc cgtggcagtc ggccgcagcg acgagaccca
     3121 tgtcgaagtc gtatcgggga ttaaggccgg ccagcgatac gtctccaagg ggggattcac
     3181 tctgcaggcg cagttgtccc ggcatggatt tggcgacggg cacgcccact gaaaggcgat
     3241 gcaacatgca aggcatcatt catttcgtat tacggaatcg actcctgatg gggatctttg
     3301 gttttcttct cttggcaggg ggatattata gctataccag gcttccagtg gatgccttcc
     3361 ccgatgtcac cccggtcctg gtccaggttt
//
LOCUS       DASPRR010000202         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1985141, whole genome shotgun sequence.
ACCESSION   DASPRR010000202 DASPRR010000000
VERSION     DASPRR010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985141"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VEL68_07835"
     CDS             <1..1335
                     /locus_tag="VEL68_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXZ35913.1"
                     /translation="PIKPEMDGTSLAENKDPHGKRLFVAFVDVCRKNGEGFVDYMWPK
                     PGSPKPEPKISFVKLYPEWGWIVGSGVYLDDVAKEVSLLLYMIFGAAGAIGAGSLVLA
                     YWMARSISRPINRIIEGLNDGAEQMASGSTQVASASQSLAEGASEQAAGLEETSSSIE
                     EMASMTKNNAENAGQANTLMVDTSKVVEGANQAMGELNKSMNEISLASEETSKIIKTI
                     DEIAFQTNLLALNAAVEAARAGEAGAGFAVVADEVRNLAMRAAEAAKNTANLIEGTVK
                     KVKNGSEIVTRTNEAFVKVVQGAKKVEGLVAEISAASQEQAQGVTQINKAMAEMDKVT
                     QQNAANAEESAAAAQEMSAQAEQMRAYVQEMNVLVKGKRNGTAAAADFQLAEEKRSPK
                     PGKTQELKSGLKKMLKSPESKGEQGKKPVKAAVKEVKPSQVIPLEEGDFKEF"
     gene            1423..2235
                     /locus_tag="VEL68_07840"
     CDS             1423..2235
                     /locus_tag="VEL68_07840"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXZ35914.1"
                     /translation="MTRQPTDIILGRRALDILRYIIDSRGPCKVSIPDTPLCWTTLLL
                     ALEREGSVDLLLVQGNPELIKALSLSGKGEVLLEYSDENGVLCHFMIEVIRTSPERVW
                     GKIPAVIYRVQRRMFFRVKAQEGTEIVFTLEPGIEVRGGVRDYSLGGVAFWSAEPLPL
                     KIEDLVEDLFLRIPEEGTWFEVHIYGAVVRRVEPGSRQGKSLYALELLGMSRRTRRTF
                     ASHILGRKRMLLRKFKVPPFLLGGPRILQRVIPAGPSAAVGKRPPYLSAWSE"
     gene            2440..2631
                     /locus_tag="VEL68_07845"
     CDS             2440..2631
                     /locus_tag="VEL68_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXZ35915.1"
                     /translation="MPNIVTAKEVGQYLKLSESTIYKLASNGEIPGFKIGDSWRFDME
                     DILKLIRDSKKEPSSQKGE"
     gene            2688..3140
                     /locus_tag="VEL68_07850"
     CDS             2688..3140
                     /locus_tag="VEL68_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXZ35916.1"
                     /translation="MDREGKYLTFALGKEEYGIGILKVKEIIGMMPVTAVPRTPGHVR
                     GVINLRGKVIPVVDLRLKFGMEEMDNSERTCIIVVEIGGAAGSVLTGIVVDAVSEVLN
                     IKGGDIEDAPAFGSKLNTDYILGMAKMNGGVKILLDIDRVMGNGEMAF"
     gene            3355..3816
                     /locus_tag="VEL68_07855"
     CDS             3355..3816
                     /locus_tag="VEL68_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXZ35917.1"
                     /translation="MKKMFALVLVQILVMGFFGFALAADNGTPSEAEAMVKKAIAYMK
                     ANGKEKAFAEINNSKGQFTDRDLYIFVYDLNGKCVAHGFNQKMIGKDLLEMKDSDGKY
                     YVKERIEIVKTKGSGWQDYKFTNPLSKKLEAKTAFVEKFEDLILGCGAYKK"
     gene            3895..5391
                     /locus_tag="VEL68_07860"
     CDS             3895..5391
                     /locus_tag="VEL68_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXZ35918.1"
                     /translation="MTGLIANLKISKKLFIGPLLVMVFLLLLGFVSYSGLSSQKSSIE
                     DIFNNRFKGYQTSASILKDLSQVHANLYKVISWANANYDAKKVESLGKEQLTVVEGTL
                     QTMGRALEDKNTTSEGKDLYQKSLAHLKEYNKTAASAVDLAASDLNMATMYMNTTDEK
                     FQVLHGSLQQILALEDRLSKEKFEYSLKSFSSSIGILIAVLGAALSLSLLVNVLITRL
                     ITSPVHETIEAVDKMAAGDLTQAIGISSKDEIGDLARSVDTMRRKMEEAVGGAVATSQ
                     TLSEAASQQAASLEETSSSLEEMASMTRKNAENTGQASQLITHSSEVVGRANSSMAEL
                     TRSMQEIAGASEQTQKIVKSIDEIAFQTNLLALNAAVEAARAGESGAGFAVVAEEVRN
                     LALRAAEAAKSTAGLMGEIVQKVKRGEELVGVTAKEFGEVAGSSEKVRQLMGEITAAS
                     QEQSQGIDQINKAVAEMNKVTQQNAGSAQELASAMAVFRVKEALGEGS"
BASE COUNT         1523 a         1433 c         1804 g         1143 t
ORIGIN      
        1 ccgatcaagc cggagatgga cggcaccagc ctggcggaaa acaaagaccc acacggaaag
       61 aggttgtttg ttgcctttgt ggatgtctgc cgaaaaaatg gagagggctt tgtggattac
      121 atgtggccta aaccgggttc ccccaagccc gaaccgaaga tctccttcgt aaagctgtac
      181 ccggaatggg gatggatcgt ggggtcgggc gtctacctgg atgacgtggc aaaagaagtt
      241 tccctcctcc tctacatgat ctttggggcc gccggagcga tcggggcagg cagcctggtc
      301 ctggcttatt ggatggcccg ctccatctcc cggccgatca accggatcat cgaggggctc
      361 aacgatgggg ccgagcagat ggcttccggc tccacccagg tggcctccgc cagccagtct
      421 ctggccgagg gagcctcgga acaggcggcc ggcctggagg agacctcctc ctcgatcgag
      481 gagatggcct ccatgaccaa gaataacgcc gagaatgccg gccaagccaa caccctgatg
      541 gtggatacca gcaaggtcgt agagggggcc aaccaggcca tgggagagct caacaagtcg
      601 atgaatgaaa tctccctcgc cagcgaggag acgagcaaga tcatcaagac cattgatgag
      661 atcgccttcc agaccaacct cttggcatta aatgccgcgg tggaagcggc ccgggccggg
      721 gaagccgggg cgggatttgc ggtggtggcc gatgaggtga ggaatctggc catgcgggcg
      781 gcggaggcgg ccaagaacac ggccaacctg atcgagggaa cggtgaagaa agtcaagaat
      841 ggatcggaga tcgtaacccg aacgaatgag gcctttgtca aagtggtcca aggggccaag
      901 aaggtcgagg gactggtagc cgaaatttca gcggcctcgc aggagcaggc gcagggagtc
      961 acccagatca acaaggccat ggccgagatg gacaaggtga cccagcagaa tgcagccaat
     1021 gccgaggaat ccgctgctgc ggcgcaagag atgagcgccc aggcggagca gatgcgggcc
     1081 tatgttcagg agatgaacgt cctggtaaag ggcaagagga acggaacagc ggccgctgcg
     1141 gactttcagt tggccgagga aaagcggagt cccaaaccgg gcaaaaccca ggagctgaag
     1201 agcggtttga agaagatgct gaaatccccg gagagcaagg gagagcaagg gaagaagccg
     1261 gtgaaggcgg ccgtcaaaga agtcaaaccc tcccaggtca tccccctgga agagggggat
     1321 ttcaaggagt tttgaaggaa ccaggaccca tgaaagggaa tattcggcgg atggctaacc
     1381 tttgctggcg cattccagag catccgccga tgccttgatt ccatgactcg tcagcctacg
     1441 gacatcattt taggccggag ggccctggat atactccggt atatcatcga ttcccggggt
     1501 ccgtgcaagg tctcgattcc cgacacgccc ttgtgctgga ccaccctcct gttggccctc
     1561 gaaagggagg ggtcggtgga ccttctgctc gtccagggaa acccggagtt gataaaagcc
     1621 ctctctttgt ccgggaaggg cgaggtcctc ctggaatatt cggatgaaaa cggggtcctg
     1681 tgccacttca tgattgaggt gatccggact tcgccggaaa gggtctgggg gaaaataccg
     1741 gcggtcatct accgggtcca gcgccgaatg ttctttcggg tgaaagccca ggagggaacg
     1801 gagattgtct ttaccctcga gccggggata gaggtgcggg gaggcgtgcg ggactacagc
     1861 ctgggggggg tagctttctg gtccgcggag cctttgcccc tgaagattga agacctggtg
     1921 gaagacctct tcctccgaat tccggaggag ggaacctggt tcgaggttca tatttacggg
     1981 gccgtggtcc ggcgggtgga gccggggagc cggcagggga aatccctgta cgccctggag
     2041 ttgctgggga tgtcccgcag gacaagaagg acttttgcca gtcacattct gggaaggaag
     2101 aggatgcttc tgcggaaatt caaggtcccc ccattcctcc tgggaggtcc ccgaattttg
     2161 cagagggtaa tcccggctgg tccctccgca gccgtcggca agcgaccgcc gtatttatcg
     2221 gcttggtcag agtgatcccc gcttctttcc agtcatttta attaggcgga ttgcgggttt
     2281 ctacttttgc cccgtttccg gtttctctaa ggaatgattc atctaagcct tttaaggtcc
     2341 gtcggggtag ggggattcca aggatttagg aaatcctcct tcccaaaaag gactcaaccg
     2401 cttgaccgaa agatcgttgg gtcttcaaga gaggtggata tgccgaacat cgtgaccgcc
     2461 aaagaagtag gacagtactt gaagttgagc gaatcgacga tttacaagct ggcctccaac
     2521 ggggaaattc ccgggttcaa gatcggggat tcctggcgtt tcgacatgga agatattctg
     2581 aaattgattc gagattccaa aaaggagccc tcgagtcaga agggagagta aaaatggctg
     2641 agacggcaaa ggcagtagcc cccgctgggg gcgcgggaac gggaataatg gaccgggagg
     2701 gcaaatatct gaccttcgcc ctggggaagg aggagtacgg aatcggcatt ctgaaggtga
     2761 aagagatcat cggaatgatg ccggtcaccg ccgttcctcg gactccgggc catgtaagag
     2821 gagtgatcaa cctgcgggga aaagttatcc cagtagtgga cctgaggctc aagttcggga
     2881 tggaagagat ggataattcg gagcggacct gcatcatcgt ggtggagatc gggggagccg
     2941 ccggttcagt cctgaccggg atcgtggtgg acgcggtctc cgaggtcctg aacatcaagg
     3001 gaggagacat tgaagacgcc ccggctttcg gaagcaagct gaataccgat tacatcctgg
     3061 ggatggccaa gatgaacggt ggggtcaaga tcctcctgga tatcgacagg gtgatgggaa
     3121 acggagagat ggccttctag tggcgcgaaa ttaggggttt ctgagtagag cattcctgga
     3181 aattccagga ttccagccgg agtgatttca aagaattgaa aggggaactc gggaaatgag
     3241 gggcagcctt agggctgaga aaaggaacta acgagaaaaa agaagacctc agcagcaggc
     3301 cccacaggcc gcgatctgac gaattccaat catgtattag gaggataaat caaaatgaaa
     3361 aagatgtttg cgctagtcct ggttcaaatt ctggttatgg gattcttcgg ctttgctctg
     3421 gccgctgaca acggcacgcc ttcggaggct gaggcaatgg taaaaaaagc catcgcttat
     3481 atgaaagcca acgggaagga aaaggctttc gcggagatca ataactccaa aggacagttt
     3541 acggacagag acctgtacat ctttgtctat gatttgaacg ggaaatgcgt ggcccatgga
     3601 ttcaatcaga agatgatcgg aaaagacctg ctggagatga aggactcgga tgggaagtac
     3661 tatgtcaaag aaagaattga gattgtcaaa accaaagggt caggctggca ggattataaa
     3721 ttcacgaatc ccctaagcaa aaaactggaa gccaaaaccg ctttcgtgga gaagttcgaa
     3781 gacctaatcc taggctgcgg ggcctacaaa aagtgattca ggacggcggc tgaacaggct
     3841 caccgctatt ccgccgccag gttttaaaag gggagaaata gaggggggaa agaaatgacg
     3901 ggcctcatcg ccaacctgaa aatatcaaaa aaattattta tcggacccct gctggtcatg
     3961 gtctttttgc ttctcctggg attcgtatcc tactccgggc tttccagcca aaaatcctcg
     4021 attgaggata tcttcaataa tcgcttcaag ggataccaaa ccagcgccag tatcctcaaa
     4081 gacctatccc aggttcatgc caacctttat aaggtcatta gctgggcgaa cgccaattat
     4141 gacgctaaga aggttgaatc ccttgggaaa gagcagctga cggtggttga agggaccctt
     4201 cagacaatgg gaagggcctt ggaggacaag aatacaacct ccgagggaaa agacctctat
     4261 cagaaatccc tcgcccattt gaaagaatat aacaaaacag ccgcttcagc ggtcgatttg
     4321 gcagcgtccg acttgaacat ggccaccatg tacatgaaca caacggatga aaaattccag
     4381 gtcctccatg gaagcctgca gcagattctg gccctggaag acaggctgag caaggaaaag
     4441 tttgaatatt ccctgaaaag tttctcttcc tccataggga tcctgatcgc ggtcttgggg
     4501 gccgccctca gtttgtccct cctggtgaat gttctgataa cccgcctcat tacctcaccg
     4561 gtccatgaaa ccatcgaagc ggtggataaa atggccgccg gtgatttgac ccaggccatc
     4621 gggatttctt ccaaagatga gatcggagac ctggcccggt cagtggacac aatgcgaagg
     4681 aagatggaag aggcggtagg aggcgctgta gccacatccc agacgctttc cgaagccgct
     4741 tcccagcaag cagcctctct ggaggagact tcctcttccc tggaggagat ggcctccatg
     4801 acccggaaga acgcggagaa cacgggccag gccagccaac tgattaccca ctccagcgag
     4861 gtggtaggca gggctaactc ttccatggct gagctgaccc gatccatgca agagatcgcc
     4921 ggggccagcg agcagaccca aaaaatcgtc aagtccatcg acgagatcgc cttccagact
     4981 aacctcctgg ctttgaacgc ggcagtggaa gcggcccgtg ccggagagtc gggagcggga
     5041 tttgccgtgg tggccgagga ggtaaggaac ctggccctgc gggcggcaga ggcggccaag
     5101 agcacggctg gtctgatggg ggagattgtg cagaaggtga agagggggga ggagttggta
     5161 ggggttacag cgaaagagtt cggggaggtg gcaggcagtt cggagaaggt aaggcagctg
     5221 atgggagaga taaccgccgc ttcccaggag cagtcccagg ggattgatca gatcaataaa
     5281 gcggtggcgg agatgaacaa ggtaacccag cagaatgccg gcagcgccca ggagctggct
     5341 tccgccatgg ccgtctttcg agttaaggaa gcccttggag agggctcttg atcggctttt
     5401 gatggccttc tcccaaacag ggagagacgc aatcggtcca ttctaattga ttcaacggcc
     5461 gaaatcataa aggatgcaag ggtcaaaaag gtcctgcact ccctggccct ttcggggcgg
     5521 ttatgcaaga tgaagagaga agaacccttg aagctctccc cttttgctaa tgattggact
     5581 cctacaaaag ctccatggtc ttcaaaaata gcaggatgct gaaaaacctg catccaccgt
     5641 cactcccgcg gaaacggggg tctagaaggc tttgaagaaa ctggattccc gccttcgcgg
     5701 gaatgacgac caacaaccga aaaggatttt ttcagtaagc tgaaagaata aaatcgagga
     5761 agaaaattca atgagcgagg aaaaaagcca atgagtatga aggcggaact cattaatccc
     5821 tttttggaag ccaccctttc ggttttgaag accatggcct ctgccgaggc agtgcccgga
     5881 aaaccctata tcaagaaggg ttc
//
LOCUS       DASPRR010000203         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_364669, whole genome shotgun sequence.
ACCESSION   DASPRR010000203 DASPRR010000000
VERSION     DASPRR010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364669"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VEL68_07865"
     CDS             <1..1120
                     /locus_tag="VEL68_07865"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HXZ35919.1"
                     /translation="GLEFLAAQNFAPLAGKRVGLITNQTGRDSSGRRTIDLLYTAPKV
                     KLKAIFTPEHGLSGQAEGKVLSGRDEATKVRVYSLYGDDLRPTAKMLKGIDVLIFDLQ
                     DAGARFYTYLSTLGYAMEAAAQNGIPFYVLDRPNPINASLVQGPVMDGEFKSFTGYFP
                     LPWRHGMTLGELARMFNEEDKIGAQLKVIPMIGYQRIAWFDETGLPWVNPSPNLRSLT
                     QTALYPGMALVEGANVSVGRGTDKPFELLGAPWIKAQELADDLNSRNILGVEFRPATF
                     TPDGNRFKGQVCYGVQVMLTDRQVLDSAILGVEIISALWRLYPQDFEIDKTLSLVGSR
                     LVHQALKEGQDPHVISQNWQAPLAEFQTLRSRYLLYPK"
     gene            complement(1274..>2089)
                     /locus_tag="VEL68_07870"
     CDS             complement(1274..>2089)
                     /locus_tag="VEL68_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ35920.1"
                     /translation="YAGRGGPASFAISAIDIALWDMAGKRRGEPLWHLLGGNNPRVKV
                     YAGGIDLFFTLDALKEQTRRFLAQGYGAIKMKVGRPKLSEDLARVAAIREFLGPDIPL
                     MVDANMRWTVEQALRASRALAEYDIYWLEEPTIPDDLPGHIRIAKEGPLPITTGENMH
                     TLYEFQNFITAGAVSFPEPDVSNCGGISIWMKVARLAEAHNLLVTSHGVHDLHVHTLA
                     AVPNASYLEGHGFGLEKYIIHPLRIEKGEAIAPDRPGHGVELVWDKLEEHRLK"
BASE COUNT          428 a          589 c          600 g          472 t
ORIGIN      
        1 cgggctcgag ttcctggcgg cccagaactt cgcccctctg gccggaaagc gggttgggtt
       61 gattaccaat cagacgggaa gggattcttc cggacggcga accatagacc ttctctatac
      121 cgctcccaag gtcaagctga aggccatctt cacccctgag catgggcttt cgggccaagc
      181 cgaaggcaag gttctttccg gtcgggatga agccaccaag gtgcgcgtgt acagcctcta
      241 tggagacgac ctgcgcccca cggccaaaat gctcaaaggg atcgatgtcc tgattttcga
      301 tttgcaggat gcaggggccc gtttttatac ttacctctcc accctgggat acgccatgga
      361 ggctgcggcc cagaatggaa tccctttcta tgttttagat cggccgaacc cgatcaatgc
      421 cagcctggtg cagggaccgg tgatggatgg agagtttaaa tctttcaccg ggtatttccc
      481 tctgccctgg cgtcacggca tgaccttggg cgagctggct cgtatgttca atgaggaaga
      541 taaaattggg gcgcagctga aggtgattcc gatgattggg taccagcgaa tcgcttggtt
      601 tgatgaaacc ggacttccct gggtgaatcc atcgcccaat ttgcgttccc taacccaaac
      661 ggctctgtat cccggcatgg cattggtgga aggggccaac gtgagcgtgg ggagaggaac
      721 ggataagccc tttgagcttc tcggggctcc atggatcaaa gcccaggaat tggcggatga
      781 tctgaatagc cgcaatatcc tgggagtgga attccggccg gctaccttca cacccgacgg
      841 taaccgattt aaggggcagg tgtgttatgg ggtccaggta atgctgaccg accgccaggt
      901 tttggattcg gccatcttgg gggtcgaaat catcagcgcc ctctggcggc tctaccccca
      961 agatttcgag atcgacaaga ccctctccct ggtcgggtct cgcctcgtgc accaggccct
     1021 caaagagggc caggacccgc atgtcatctc ccagaattgg caggcccctc tcgcagagtt
     1081 ccaaaccctg cgttctagat acctactcta cccgaagtag cctgaaatag gcagcctgaa
     1141 ggccgcgatc gcatttccct gtccttcgag gcacaaaccc accaggggat ttcattctcc
     1201 attccgcccg gtagtcaaaa gggtgtatgg cgatccagaa tctcgcgcgg ttcaagtcgc
     1261 atttcccatt tttttatttt agccggtgtt cttccagctt gtcccagaca agttccactc
     1321 catgtccggg gcggtccgga gcaattgctt ctcccttttc gatgcggagg gggtggataa
     1381 tatacttttc caggccgaat ccatgcccct ccaggtagga ggcgttggga actgcggcaa
     1441 gagtgtggac gtgcaggtca tggactccat gcgaggtcac cagcaggttg tgggcttctg
     1501 cgaggcgggc caccttcatc cagatggaaa ttccgccgca gttggaaacg tcgggctcag
     1561 gaaaggaaac ggctcctgcg gtgatgaaat tctgaaactc gtatagggtg tgcatattct
     1621 ccccggtggt gatgggcagg ggcccttcct tggcgattcg gatgtgtcca ggcaggtcgt
     1681 cgggaatcgt cggttcttcc aaccaataga tgtcatattc tgccaatgcc cgggaggccc
     1741 gaagggcttg ctcgacggtc cagcgcatgt tggcgtccac catgagggga atatccggcc
     1801 ccaggaattc tcgaatggca gcaacacggg cgagatcctc cgataacttg ggacggccca
     1861 ctttcatctt gatggcccca tatccctggg ccaggaatct tctggtctgt tcctttaggg
     1921 catccagggt gaagaagagg tcgattcccc cggcatagac cttcacccgc ggattgttcc
     1981 cgcccagtaa atgccagagg ggttctcccc ttctttttcc ggccatatcc cacagagcaa
     2041 tgtcgatcgc cgagatggcg aaggaagccg gccccccgcg tccggcata
//
LOCUS       DASPRR010000204         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2305330, whole genome shotgun sequence.
ACCESSION   DASPRR010000204 DASPRR010000000
VERSION     DASPRR010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305330"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VEL68_07875"
     CDS             complement(<1..660)
                     /locus_tag="VEL68_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXZ35921.1"
                     /translation="MKTRVLLVILALLSGFISQGAVAKDLTKIVVWDLAPRETKPGYA
                     QELTSILVSEINKLGKYEIYSQENVRTLAGWTAEKMKLGCTSTQCLTALGQMDVAKLI
                     SGSVGKIGNRYSVSLSLFDTQNAKAEKSISEFCRTEDELIELVQKAGRQLLGVPVDLA
                     AAEKKELDKTFTNSMGMEFVLIPAGKFIMGSPANEAGRYENEGPQREVQIAKPFYLGK
                     YE"
     gene            complement(812..1900)
                     /locus_tag="VEL68_07880"
     CDS             complement(812..1900)
                     /locus_tag="VEL68_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35922.1"
                     /translation="MKKGFSYQYNRIFASSAHSSYDEEEAIFPGRFQQTWLYVFIAIV
                     LVVPFISGEYGLYLLNLWLLQLIAAMGLNLLTGYTGLLSLGHAAFMGIGAYTMALLTT
                     QAGFPLLLALPFSGAVAALFGVLIGVPSLRIKGFYLMVATIAFQFLMDYVLVHWERVT
                     RGIRGIELSTPTLLGIPLKTNFAFYFLALTIAAFMLWGAKNLIRSRIGRAFMAVRDND
                     ISAQIIGIPVFPYKLLSFLISAFYAGIAGALWALLYRNAVPEHYTLMPSVQYLVMVLV
                     GGLGSIVGTILGVGFILLLPEGLNLLVSYLARTIHPDLMVFLAPAKSILFGALIVLFI
                     LFEPEGLVGVWRRFRDYFKIWPLPYISS"
     gene            complement(1897..>3789)
                     /locus_tag="VEL68_07885"
     CDS             complement(1897..>3789)
                     /locus_tag="VEL68_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ35923.1"
                     /translation="MVTDTLIQLFISRAKRHPDRIALRERQYGIWIPTTWAELWQKSA
                     SFGLFLKERGLEAGECVILWGDNKPEWLIAEMGTLAAGGVISGIYPDSLPEEAKYLIE
                     ATDAKFIVVGDQEQADKLLKVWEEISPRLKVVIVWDSRGMNHYYAQHPFLLRFEDCLR
                     EGSKGKHVALEEVVPSSAEQIAMMLTTSGTTAKPKLALLSHRNLIFLARSFGQVVPMD
                     ESDDILSLAPMAWIGEQLYNVVRFLDCGFRYNFPEEAETLRRDLVNLQPTYFGGTPSR
                     WEDIASSIQAAIDNADFLKRNAYRWAINLCQRAVEKELAGEDIGSWNRYLRNLARFLV
                     IRPLTKQVGLGRAKIAITGGAAISPEVFKYFKALDLDMRQIYGQTECTAIATCHREGD
                     VRPESAGVALPGVTLKISEAGEIFVGGEGIMAGYYKNPEATSQAITPDGLLRTGDAGY
                     LDKDGHLFVFDRYKDVMHLAGGTRFAPQDIETRLKFSPYIKEAVVIGDQRPYISALVS
                     IDLENVGNWVKKRGVAYTTFHDLSQKPEVYQLVQSEIGRLIAAFPDPMKVKRFANLIK
                     ELLPDDEELTRTRKVRRALVYERYDSLIRSIYGGEKEHILDVRIRFEDGHEATFNGRV
                     KIADVE"
BASE COUNT          821 a         1086 c         1096 g          786 t
ORIGIN      
        1 ctcgtatttg cccaagtaaa aaggtttggc gatctgcact tcacgctgag gcccttcatt
       61 ctcgtatcgc ccggcttcat tagctggact ccccatgatg aatttccctg cgggaatcag
      121 gacaaactcc atacccatag agttagtaaa ggttttatcc agctcttttt tctcagcggc
      181 ggccaggtcc accgggacac ccagtaattg gcgtcccgcc ttttgaacca attctatcaa
      241 ttcatcctct gttcggcaga actcggagat agatttttca gccttggcgt tctgggtgtc
      301 aaagaggctt agggaaaccg aatatcgatt cccgatcttt cctacgctcc cggagatcaa
      361 tttggccaca tccatctggc caagcgccgt gaggcactgg gtgctggtac accccagttt
      421 cattttttcc gcggtccacc cagccagggt tcgtacattc tcctggctgt aaatttcata
      481 ctttcccagc ttgttgatct cactgaccag aatggaagtg agttcctggg catagccggg
      541 tttggtctcc cgcggggcga ggtcccagac cacgatcttg gtaaggtctt tcgccactgc
      601 accctgggag atgaagccag agaggagcgc caagattact aacaacaccc tggttttcat
      661 ccttcctccc ctttaaggac ttgaaatgac acccagaacg gacccttccg caaaattcca
      721 tctcgatatt atttcaacca agcagatact atccgaattg atctgaggag tcaatgatgg
      781 gggaacctca gtctgagaag gctgcacccc ttcaagagga gatatagggc aatggccaga
      841 ttttgaaata atcccggaat cggcgccaga cgccgaccag gccttcgggc tcgaagagga
      901 tgaagaggac gatcagcgcc ccgaagagga tcgatttggc cggggccagg aagaccatta
      961 ggtccgggtg gatggtgcgg gccaggtagc tcaccagcag gttcagcccc tccggtagaa
     1021 gcaggatgaa acccacgccg agaatcgtcc ccacaatgct tcccaaccct cccaccagga
     1081 ccatgaccag gtactggacc gaaggcatga gggtgtagtg ctcaggaaca gcattgcggt
     1141 agagcagggc ccacagggcg ccggcaatcc cggcatagaa ggcgctgatc aaaaaagaga
     1201 gcagcttgta ggggaatacc gggatgccga tgatctgggc ggagatatcg ttatccctca
     1261 cggccatgaa ggcccggccg attcgtgagc ggatcaggtt cttggctccc cagagcatga
     1321 aggcggcgat ggttagggcc aggaaataga aggcaaagtt cgtcttcaaa ggaatcccca
     1381 gaagggtcgg agtggagagc tcgatgcccc ggatcccccg agtcaccctt tcccaatgaa
     1441 ccagcacata gtccatgaga aactggaagg caatcgtggc caccatcagg tagaacccct
     1501 tgatccgcag ggagggaact ccgatcagca ccccgaataa ggcggccacg gccccgctga
     1561 agggaagggc gaggagcaag ggaaacccgg cctgggtcgt cagaagcgcc atggtatagg
     1621 ccccgatccc catgaaggcg gcatggccca gggaaaggag gccggtatag ccggtgagaa
     1681 ggttgagccc catggcggcg atgagctgaa ggagccagag gttgaggagg taaaggccgt
     1741 actccccgga gataaaggga accaccagga cgatggcaat aaagacatag agccaggttt
     1801 gctgaaagcg gccggggaaa atggcctcct cctcgtcata gctggagtgg gcggaagagg
     1861 cgaagattcg gttgtactgg taggaaaatc cttttttcat tccacgtccg cgatcttgac
     1921 ccgaccgttg aatgtggcct cgtgaccgtc ctcgaagcgg atccggacat ccaggatgtg
     1981 ttctttctcc cccccataaa tggagcgtat cagggaatca tacctctcat agaccagagc
     2041 ccggcggact ttgcgggtcc gggtgagttc ctcatcgtcc gggagcagct ccttgatcag
     2101 gttggcgaat cgtttgacct tcatgggatc cggaaaggcg gcgatcaatc tgccgatctc
     2161 gctctggacg agctgataga cttcgggctt ctgcgaaagg tcgtggaaag tggtgtaggc
     2221 cacccctctt ttctttaccc agttgcccac gttttccagg tcgatgctga ccagggcgct
     2281 tatgtaaggg cgctggtccc caatcaccac cgcttccttg atgtaggggc tgaacttcag
     2341 tcgggtctcg atgtcctggg gagcaaagcg ggttccgcca gccaggtgca ttacgtcttt
     2401 gtaccggtcg aagacgaaga gatgcccgtc tttgtccagg taccccgcgt ccccggttcg
     2461 caggagaccg tcgggagtga tggcctgtga tgtggcttcg gggttcttgt agtatcccgc
     2521 catgattcct tcgcccccca cgaagatctc cccggcctcg gagatcttca gggtcacccc
     2581 tggcaaggcc accccggccg attccggacg cacatctcct tcccggtggc aggtggcgat
     2641 ggccgtgcat tcggtctggc cgtaaatctg ccgcatgtcg aggtccagcg ccttgaagta
     2701 cttgaacacc tccggggaga tggccgcccc cccggtgatg gcgatcttcg cccttcccaa
     2761 tccgacctgc ttggtcagcg ggcgaatgac caggaaccgg gcaaggtttc gcaggtaacg
     2821 attccaggaa ccgatatcct ctccggccag ctctttttcc acggctctct gacagaggtt
     2881 gatcgcccag cggtatgcgt tccgcttcag aaagtccgcg ttgtcaatgg ccgcctggat
     2941 ggaggaggca atatcctccc atcgggaagg ggtcccccca aaataggtgg gttgtaggtt
     3001 gaccaggtcc ctccgcaggg tttccgcctc ctcgggaaag ttgtagcgga agccgcagtc
     3061 gagaaaacgg actacgttgt agagctgctc cccgatccag gccatgggcg ccagggagag
     3121 aatatcatcg ctctcatcca tggggacgac ctggccgaag ctgcgggcca ggaagatcag
     3181 gttgcggtgg ctcaaaaggg cgagcttggg tttggccgtg gttcccgagg tggtcagcat
     3241 catggcgatc tgctcagcgg aagaaggaac aacctcttcc agggcgacgt gtttcccctt
     3301 cgagccctcc cgcagacaat cttcgaagcg gagaaggaaa gggtgctggg cataatagtg
     3361 gttcatgccc cgggaatccc atacgatgac cacctttaac cggggggaaa tttcctccca
     3421 gaccttgagc agcttgtccg cctgctcctg gtccccaacc acgatgaact tagcatccgt
     3481 ggcctcgatc aggtacttgg cttcctctgg caggctatcc ggatagatac cgctgatcac
     3541 ccctcccgcc gccagggtcc ccatctcggc gatcagccac tcgggcttgt tgtctcccca
     3601 gaggatgacg cattccccgg cctccaatcc ccgttccttg agaaagagcc cgaaggaggc
     3661 cgatttctgc cagagttccg cccaggtggt gggaatccag atcccatact gcctctcccg
     3721 cagggctatc cggtcaggat ggcgcttcgc ccgggaaatg aacagctgga tgagggtatc
     3781 ggtcaccat
//
LOCUS       DASPRR010000205        16462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2305557, whole genome shotgun sequence.
ACCESSION   DASPRR010000205 DASPRR010000000
VERSION     DASPRR010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16462)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16462
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305557"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VEL68_07890"
     CDS             complement(<1..1163)
                     /locus_tag="VEL68_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35924.1"
                     /translation="MGKRFITVFLTGALIVGMIGISYSNPGYMSQFNTNYGTSGTALN
                     SCSVCHTSPPTLNPYGNDWLNSAYNFAAIEGLDSDKDGFTNIVEIQARTFPGDAASHP
                     AAAGDTTPPTVTGFTLPPTAAALTVSILAFTATDNVGVTGYMVTESATAPAAAAAGWS
                     AAPPSSYAFGTAGTKTLYAWAKDAAGNVSASLSASVTVTIATPDTTPPTVTGFSIPAT
                     ATRLTVTITTFTATDNVGVAGYLLTESATAPAAAAAGWSPAPQSFYRFASAGGKTLYA
                     WAKDAAGNVSTSLSASVNITLSGTPTGPAALGIYRGGEWYWDNNGNGIWDGCAVDSCK
                     QSFGSPDDIPVAGDWTGDGIKKIGVYRKGSWYLDLNNNGVWDGCGVDACIASFG"
     gene            complement(1576..2460)
                     /locus_tag="VEL68_07895"
     CDS             complement(1576..2460)
                     /locus_tag="VEL68_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ35925.1"
                     /translation="MEERVITRRDFLRGAAATAVAAAMGPGILGEAQAQPAAKVVLIR
                     NADVLAQPDKMQGILESMLDEALKTLLGTSEPMEAWRKLVKSSDVVGVKSNEWPKLPT
                     PKPLEGAIKRRLLDVGVVEQNISIADRGVLENPIFQKATALVNVRPLRTHHWAGIGSC
                     LKNYIQYAPNRSSYHPEACAALGSIWKLPMVEGKTRLNIQCALTPQFYGRGANFFDTR
                     YVWPYQGLIVGTDPVAVDTVAAHLLQVKRTAFFGEDRALDVPPSHIIVADKKYHLGVS
                     DLNRIQLVKLGWMEDALI"
     gene            complement(2776..4257)
                     /locus_tag="VEL68_07900"
     CDS             complement(2776..4257)
                     /locus_tag="VEL68_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HXZ35926.1"
                     /translation="MGRRILSAEISHETNTFSILPTTLSLYNARRYYRWEEISRAFSG
                     TRTEMGAHLDAAKKYGWEMVQPIAAAATPGGKTPAKDWAHLSGAVLEACEEGPFAGVL
                     LSLHGAMVTEDQDDAEGDLLRRLRAKLGPDVPIAISLDIHANVSNAMARQANIVIAYR
                     TYPHIDQYERGMEAADLLQRTMNGEVRPRSLVWRAPVLEGLDYGRTQGGPMSRILAHA
                     DQLRQKNPGVLAITVCAGFVWADIPDAGPSVTITAHGDPSRFKGAAEELLKDIWETRR
                     EITVPLYSLREAMTEAGKESKDGRPLILADFTDNPGSGGYGDSVRLLEAMIQADLDNA
                     AFGCIPDPEAAQKCIAAGVGKEIEMALGAKIDPKTYGPPLMIRGMVEQVSDGKFIAQG
                     PMSKGVRLSMGPTAVLRCGGVRALVTTHNIQVYDLQVFLSQKIDPRACSVVAVKSWHH
                     FRASFEPISRAVMLVDSGGLASRDLKRFTYHKIRRPIYPLDLE"
     gene            complement(4484..5443)
                     /locus_tag="VEL68_07905"
     CDS             complement(4484..5443)
                     /locus_tag="VEL68_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005893344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ35927.1"
                     /translation="MNPSLQPILELIDVYRTFMVARGLFKGKKELHAVNGVSLQIQKG
                     DVLGLVGESGCGKTTLARILLGLLPPSSGQILIDGKVIKRGERRSIARKIQPVFQDPY
                     SSLNPRKTIGSIISLPLRAHKVGDSSTWRGQVEEILDLVGLPRRVLHSYPGQLSGGQR
                     QRVAVARALIMRPEVVICDEPTSSLDVSVQAQILNLLISLRKELGLTYLLISHNLAVV
                     EHLATRVAVMYLGRIVEEAETDRIFKKPSHPYTKALLESVLTPEPGLGVPELHLGAGF
                     PNPLDPPSGCTFHPRCVYAKPDCGERVPSERRDEKGYVACHLY"
     gene            complement(5545..6549)
                     /locus_tag="VEL68_07910"
     CDS             complement(5545..6549)
                     /locus_tag="VEL68_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35928.1"
                     /translation="MSSLLEVENLHVDIPIVTGDLHAVRGIDFTVGKGETLGLVGESG
                     CGKSMTALALMNLLPKSAIRKASLLAFSGKDLVELKEREMADIRGDRMSMIFQEPMTS
                     LNPSYTIGNQLMEVMIRHRRVSRSEARRRAVFLLEKVGISAAKSRLGQYPHQLSGGLR
                     QRVMIAMALMCDPELIIADEPTTALDVTIQAQILHLLRGLQREFRMGMILITHDLGIV
                     ARVTDRVAVMYAGQIVETGTVRDVFTRPLHPYTYGLLDCIPIPGKTQHGEFLGSIPGM
                     VPTPVGALAGCAFRNRCSFTFQDCAIQGPGMCMVSETHGYRCLLEPEKLAKRAKSNQQ
                     "
     gene            complement(6768..7664)
                     /locus_tag="VEL68_07915"
     CDS             complement(6768..7664)
                     /locus_tag="VEL68_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35929.1"
                     /translation="MEIRPIPPVEEILGPSPGRLLRRRIFGHKGLMIGGIILAVIILF
                     ALLAPLLSPHDPYVQSLTTRLSKPFWHGEGKWNHPFGTDHVGRDYLSRLLYGAQVSLL
                     IGFCVALISGVIGTALGVVAGYFGGRVDMVITFIITLRLSIPFVLVAVAVVGIVGGSL
                     KVVIIVLGLLLWNRYAVVMRSATQQIRNQDYIPAAEAVGCSTTRILLSEVMPNIVNNL
                     VVVATLEMANAILLEAALSFLGLGVQPPTPSWGLMVSEGKDFLFFEPWLITIPGVALF
                     VLVLAINLLGDGIRDVTAPENR"
     gene            complement(7666..8583)
                     /locus_tag="VEL68_07920"
     CDS             complement(7666..8583)
                     /locus_tag="VEL68_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011537208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35930.1"
                     /translation="MLVYTLKRLALAVAVTLVVTALSFGLLYLSGDPASAIGGEAATD
                     ADIANIRKIYGFDRPIHVQYLTWVGKALRWDFGMSYYFRLPVSQIMAARIPATLILGS
                     SAIVFALVFSIPLGVIAAIRPNSWVDRTALFLSVMGQAMPSFWLGLMLIVVLSVWLPL
                     LPPSGTEGWEYFVMPTIVLGYYAMPAIMRLTRAGMMEVLASDYIRTARAKGLLPGSIL
                     FKHALRNAVIPVVSVSAVQFGFMLGGSIVVETVFAIQGMGYLAWESIKRSDFPTVQAV
                     ILVFALIYIVLTLLADLLNGWLDPRIRVT"
     gene            complement(8664..10187)
                     /locus_tag="VEL68_07925"
     CDS             complement(8664..10187)
                     /locus_tag="VEL68_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019361460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35931.1"
                     /translation="MRKKFSWLCLIALFAFILMAPAAHAGKANDTLNILHEIELSSVD
                     NYFNTDRWGVVLCHLLWDSLLYRDLKTGEYKPNLATSWKYVDGKTLEFDLRQGVKFHN
                     GDEFDADDVVYTLNWISDPKNGIKSQNISNWIERCEKLGKYKVRLKTKTNFPAAPEYI
                     AAVLAIYPHKYYAKVGPDGMGLKPVGTGPYKGVEVVPGKSITLAKNENYFAASPKGKP
                     SIGKLHIRFISEVNTQIAELMSGRADWIWRVPQDQAEKMAKMPKITVEASDTMRVFFL
                     HFDAANKKGRNSPVNKLKVRQAIGHAVDKATIQKTLVKGASQLLHSACYPSQFGCTDD
                     VVKYEYDPAKAKKLLEEAGYPNGFEIEFYGWRNRPEMEAIMGYLNAVGIKTKIQFMTY
                     AATREKIRVGEVDMAMNNWGSFSIHDASAFTGNFFGGTTDDNARDPEVIKWLEIADTS
                     IDPEVRKKNYKLALQRIAEQAYCIPLWSSNMNYAYTKDLNFSAQPDEIPRFFNASWK"
     gene            complement(10449..11645)
                     /locus_tag="VEL68_07930"
     CDS             complement(10449..11645)
                     /locus_tag="VEL68_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ35932.1"
                     /translation="MTVPIAERMSRIGTETAFAVSLEASQLAQKGLKIYPFHLGDMNI
                     ITPSNVMEAAIEAMKKGKTGYCPTAGIPDLREAVAKDVGEQRKLKLTADNVSIQPGGK
                     PTIGKFILTAMNPGDKVLYPNPGYPIYESQIEFHGGVGLPYGYKEARDGFILDMDAIR
                     KGIQAGAKILIYNNYQNPTGASSPPEEMEELAALAVKNDLFVLADEAYFEIRYSGRPR
                     SIASFPGMAERTFILYTFSKKFAMTGWRLGAAVGPAKFIEYISKINVNDESCSNHFIQ
                     YGALAGLQGSNEGPLGILSILKERRDMLVDLLNDIPGVRCFKPEATFYLFPNVTGAMK
                     RKGVSDVEEFRKLVLRETGVSFCTRKHFGRPLPGETEQYIRFAYSGIDVSMIKEGMKK
                     LAAFLA"
     gene            11999..14353
                     /locus_tag="VEL68_07935"
     CDS             11999..14353
                     /locus_tag="VEL68_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35933.1"
                     /translation="MALKFGPRRGTYTDISRKMKGGGLSSDEKKVFEDFDLVYRSLCA
                     LLYNYVPASGHPGGSISSGRFVEGILFGAMDYDVSNPDREDADLISYAAGHKALGLYS
                     MWALRNEVIRISAPHLLPKEVRYQLRLEDLLGFRRNPITQTPLYSRFGSKPLDGHPTP
                     ATPFLRLSTGASGVGAASSFGLAFGAMDYYGKDAPRVHIVEGEGGMTPGRVSEAMAAA
                     GTACLKNIILHVDWNQASIDSNRVCREGNDPGDYVQWNPLEFAYLHDWNVIWVPDGLD
                     FGQVFAAQQAALRMRNTQPTAIVYRTQKGWQYGIEGRASHGAGHGLCTDGFYQALKPL
                     TDLMKGDLPRCQGGSQRCQAGKDKAVIEECFWEGLQLIRSTLEKNRPMVEPLAQRLIN
                     ARLRLNRRGRIPRANGPVIESVFEAAKGTAGTIPQNLVLQPGKNTTLRGELGRVLHYY
                     NKASNGAILTAAADLLGSTSVNTAAQGFPEGYFNASTNPGARLLSIGGICEDAMSGIL
                     AGISTFGHHVGVGSSYSAFIAPLGHISARLHSIGNQSRQAIAQEPYRPMILVCAHAGV
                     KTGEDGPTHADPQALQLLQENFPLGTLITLTPWDPQEMWTVVSAALDRRPAVIAPFVT
                     RPSEKVIDRQALGLAPATAAASGLYLLHGAQKQRAGTIVLQGSEVGYAFVEHALPLLK
                     KEGIDVDAYYVASAELFNLLPQGEREKIFPEERAREAMGITGFTLPTLDRWVLSERGR
                     RHSLHPFQKGHYLGSGQADQVMAEAGLDGESQFKAILRYVRERD"
     gene            complement(14544..16100)
                     /gene="zwf"
                     /locus_tag="VEL68_07940"
     CDS             complement(14544..16100)
                     /gene="zwf"
                     /locus_tag="VEL68_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXZ35934.1"
                     /translation="MVEKSRIEDVAVTKYLQTCDITAQSFKVEPFTLVIFGGTGDLSR
                     KKLLPALFHIYLGNELPEHFSILSLGGSKLEDDSYRALMERALRGSDEVCFSEEKWAE
                     FRSRLFYLPGNLEEEGTYQRLCSRLDQVMVPDREGHKNVIYYMAIPPQMTPTAVNLLN
                     ARGLCRGPLDTKIIVEKPFGRDFSSAVQLNRLLTSAFDERQVYRIDHYLGKETVQNII
                     FFRFSNSIFEQLWNRRYIDNVQITVAEDIGIDNRVRFYEQAGVIRDIVQNHILQLIGL
                     IAMEPPVGFEADFIRDEKTKVFRSLHPLREEQVDRFSVIGQYGPGKVQGKEVPGYRQE
                     KGVSPDSATPTFFAGSFYIANWRWAGVPFYVRTGKRLAKRVTEIAIQFDQPPLRLFGR
                     TCDMLEANVLLLTIQPEEKISLRFGVKVPGMPNQIYPIDMKFSYKETFQSAPHPAYER
                     LIQDCLKGDLTLFVRQDQIEAMWEAVDPIISCWENQPPRNFPNYAAGTWGPPEANRLL
                     EQEGKRWITA"
     gene            complement(16193..>16462)
                     /locus_tag="VEL68_07945"
     CDS             complement(16193..>16462)
                     /locus_tag="VEL68_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35935.1"
                     /translation="KHDDPKLSHLSPSPSPSIVGSNTPSFSKTHSKEGRPFNDQWFPS
                     VEVFGRRETLEEGKELFSFSPEYYPKGKRSFFSFSETSHIVYNQI"
BASE COUNT         3427 a         4743 c         4709 g         3583 t
ORIGIN      
        1 ccgaaggaag cgatgcaggc atccactcca cagccatccc agaccccgtt gttgttcagg
       61 tccaggtacc agcttccctt tctatagaca ccgatttttt tgatgccgtc tccggtccag
      121 tccccggcaa ccggaatatc atccggtgag ccgaaagatt gcttgcagga atccaccgcg
      181 caaccgtccc agatcccatt cccgttgtta tcccaatacc attcaccccc ccggtatatt
      241 cccaatgccg ccgggccggt cggcgtaccg ctcagggtga tgttgacgga ggcgctgagg
      301 ctggtggaga cattgcccgc cgcatccttg gcccaggcat agagggtttt ccctccggcg
      361 ctggcaaacc ggtagaaact ctggggtgcg ggactccacc cggccgcggc agcggccggc
      421 gcggtggcgg attccgtgag caggtaaccg gctaccccca cgttatcggt agcggtgaag
      481 gtcgtaatag tgaccgtcaa tcttgtggcg gttgccggaa tactgaaccc ggtcactgtg
      541 ggaggagtgg tatcgggcgt agcgatggtg accgtgaccg aagcgctgag gctggcagag
      601 acatttcctg ctgcatcctt tgcccaggca tagagagttt tagtccccgc agtcccgaaa
      661 gcatagctcg atggtggagc cgcgctccag ccagctgccg ccgctgcagg ggcggtcgca
      721 gattcggtga ccatataacc ggttactccc acgttatcgg tagcagtaaa ggcaaggatg
      781 gaaaccgtca aagccgcagc cgtcggcgga agggtgaacc cggtcactgt tgggggagtg
      841 gtgtcgccgg ccgcagccgg gtgacttgcg gcgtcgcccg ggaaggtccg cgcctggatt
      901 tcgactatat tggtgaatcc atccttatca gagtccaatc cctcgatggc ggcaaaatta
      961 tatgcggagt ttaaccaatc gttgccgtaa ggattaagcg tcggaggcga agtgtggcaa
     1021 accgagcagg agttcaatgc cgtacccgat gtaccataat tggtattgaa ctggctcata
     1081 taaccggggt tgctgtagga aattcctatc atcccaacga tcagcgcgcc ggtcaaaaaa
     1141 acggtaataa accttttccc cattgtctac ctcctttttt taagagccct tattgggttt
     1201 ctccgccaag aaaacgaatt cttccatccc ctgactgggc agagggggaa ttcggtcttt
     1261 cggttttttt tcggttcact ggcgggtagg tttgggggtt tagagcaagg aatatgccag
     1321 aggatgaaat tagaatggct agttaatatt gttggctatt tttgatcgat cgttcttgcg
     1381 gatggaaggt tattcccttc acatcggact ttcaatgaag ggagaatcct acaaaggtgt
     1441 aaaaaggaag ggataggttt ccaccgcgga gagtttttgc agagaactcc gtctattcct
     1501 agagaagaca gattcttaat tgacttaacc ttaatccaaa agtgctcggc acccgctctt
     1561 ccggcctgat ctggactaga tcagtgcatc ttccatccag cccagcttaa ccagctgaat
     1621 tcggttcagg tcgctgactc cgagatgata ttttttatcc gctactatga tatggctggg
     1681 ggggacgtcc aaggcccggt cttcgccgaa aaaggccgtc cgcttgacct ggagcaggtg
     1741 ggcagccacc gtgtccaccg ccaccggatc ggtccccacg atcagcccct gatagggcca
     1801 gacgtaccgg gtgtcgaaga agttcgcccc ccttccgtag aattgcggcg tgagggcgca
     1861 ttggatattc agccgggtct tcccctccac catgggaagt ttccaaatgc tgcccagagc
     1921 cgcgcaggct tccggatggt aggacgaacg gttgggggca tactggatgt aatttttcag
     1981 gcaggatccg atcccggccc agtgatgagt cctcagcggg cgcacgttga ccagcgcagt
     2041 ggctttctga aagatcgggt tttccagaac ccccctgtcg gcgatgctga tattttgctc
     2101 gaccacaccc acgtccagca agcggcgttt gatggcccct tccagcggct tcggggtggg
     2161 cagcttcggc cattcgttgc tcttcacgcc caccacgtcc gaactcttga cgagcttccg
     2221 ccaggcttcc atgggttcgc tggttcccag gagagtcttc aacgcttcat ccagcatgga
     2281 ttccaggatc ccctgcatct tgtccggctg tgccagcacg tcggcattcc ggatgaggac
     2341 gactttggcc gccggctgcg cctgagcttc tcccaggatc ccgggcccca tagcagcggc
     2401 tacggcggtt gccgcggcac cgcggagaaa gtccctgcgg gtgatgactc tttcttccat
     2461 tgaaccaagc tcctttccct tgcttctatt tcccctcatc tttcaaaacg aaagcgtcct
     2521 gctccttttt attatactcc caatcgctct ccggcgcccg gaaaaaagtt acctttccgg
     2581 ggggctgaat tgccagggtt cacttaccgg aaatgccgag cgaggaattc cacggtacgg
     2641 ataacgatcc ggcagtttgc cctgccacac tgaatcgtat ttaattttca ttccgcattc
     2701 cggattccgc cctgcactac gtagagtgcg cgggcatccc tgaaattctt gtggttcagc
     2761 gagcactcct ccaaattatt ccaaatccag tggataaatc gggcggcgga ttttgtggta
     2821 ggtaaaccgt ttgaggtccc gggaagccag gccgccgctg tccactagca tcactgcgcg
     2881 ggagatgggt tcgaaggagg cccggaagtg gtgccaggat ttgaccgcca ctaccgagca
     2941 ggcccgcgga tcgatcttct ggctcaaaaa aacctggaga tcatatacct ggatattatg
     3001 ggtggtcacc agcgcccgca cccctccgca gcgaaggacg gctgtgggcc ccatggaaag
     3061 ccggactcct ttactcatgg gaccctgggc gataaatttt ccatcgctca cctgctccac
     3121 cattccccga atcatgagcg gcggcccata ggtcttgggg tcgattttgg cccccaaagc
     3181 catttctatc tcttttccca ctccggcggc gatgcatttt tgtgcggctt ccggatcggg
     3241 gatgcagcca aaggcggcat tatctaaatc ggcttgaatc atggcctcaa gcaatcgcac
     3301 cgaatccccg tatccccccg acccgggatt atcggtgaaa tccgccagga tgagcggccg
     3361 gccatctttt gattcctttc cggcctcggt catggcttct ctcaggctgt agagggggac
     3421 cgtgatttcc cggcgggttt cccagatatc ctttaataat tcttcggcag cacctttgaa
     3481 ccgggagggg tcaccatgag ccgtgatggt cacggaaggt ccggcatcgg ggatatcggc
     3541 ccagacaaac ccggcgcaga cggtgatggc caatacacca ggatttttct ggcgcaactg
     3601 atccgcatgg gccaggattc tggacatggg ccccccctgg gtccgcccgt aatccaggcc
     3661 ttctagtacg ggcgcacgcc aaaccaggga gcgcggtcgg acctccccgt tcatggtgcg
     3721 ctggagcaaa tcagcggctt ccatgcccct ttcgtattga tcgatgtgcg ggtaggtccg
     3781 gtaggcgatg acgatattgg cctgtcgggc catggcgttc gacacgttgg catggatgtc
     3841 taggctgatg gcgatgggaa catcgggccc gagctttgcc cgcaaacggc gcaggaggtc
     3901 tccctcggcg tcgtcctggt cttcggtgac catggccccg tggagggaaa ggagcactcc
     3961 agcgaaggga ccttcttcgc aggcttccag cactgctccg ctaagatggg cccaatcttt
     4021 ggccggggtt ttgcctccgg gggttgcagc agcagcgatg ggctgcacca tctcccaccc
     4081 gtatttcttg gctgcgtcga gatgcgcccc catctccgtt cgagtcccgg aaaatgcccg
     4141 gctgatttct tcccaccggt agtatcggcg cgcgttatat aaggacaagg tggtgggaag
     4201 gatgctgaag gtgttggttt cgtggctgat ttcggcggaa agaattcgac gacccattta
     4261 actttctcct catagggcat cgttggggcg gttcgcgacc cgccccaaca ctaaaatatt
     4321 ttcccgaagc ccctgcgggc aggtttcaac cccccctacg aatcctggac tgtattccta
     4381 acggcgcgcg ccgcgcgctc ctaaaatccg caatccgcca tgcaccacat tgggtgcatg
     4441 gctgccctga accttatgcg gttcagggcg tattccgcat tctttagtac agatgacagg
     4501 ccacataccc tttttcatcc ctcctctccg aaggaaccct ttcgccgcaa tcgggtttgg
     4561 catagacgca acgaggatgg aacgtgcagc cggacggcgg atcgaggggg ttcggaaagc
     4621 ccgctcccag gtggagttca gggactccca ggccgggctc cggggtcaac accgactcca
     4681 gcagagcttt cgtgtaggga tggctgggct ttttgaagat tcggtccgtc tccgcttcct
     4741 ccacgatcct gcccaggtac atgaccgcga cccgcgtggc caggtgctcc accaccgcca
     4801 ggttatggct gatcagaaga taggtaaggc ccaactcctt tcgcaaagaa atcagaaggt
     4861 tcaggatctg tgcctggacc gagacgtcga gggaagaggt cggctcgtcg caaatcacca
     4921 cttcgggacg catgatcaaa gcccgggcca cagctacgcg ctggcgctgc cctcccgaaa
     4981 gctggcctgg atagctgtga aggaccctcc tcggcaagcc aaccaagtcc agaatctcct
     5041 ccacctgacc gcgccacgtg gaagaatctc ccactttgtg agcccgcaag ggaagagaga
     5101 tgatggagcc gatggttttg cggggattca acgacgaata ggggtcctgg aagacgggtt
     5161 gaatcttgcg ggcgatggac cggcgctccc ctctttttat gactttccca tcgataagga
     5221 tttggccgct gctgggcggc aggagcccca gaagaatccg ggccagggtt gtcttgccgc
     5281 agcccgattc ccccaccagc cccaggacat ctcccttttg aatttggagg gagaccccgt
     5341 tgaccgcatg gagctccttc ttccctttga agaggccccg ggccaccatg aaggtccggt
     5401 agacgtcaat taattcgagg atcggttgga gagatggatt catttgtggc ttccggtcaa
     5461 tagttcgtaa gggcgggttt gaaatccgcc cgtacagatt tccaacaaaa agctatcggg
     5521 gtagttaggg cctttgttca ttgctcattg ctgattgctc tttgctctct tggcgagttt
     5581 ctccggctcc agcaggcacc ggtacccatg ggtttcgctg accatgcaca tccccggacc
     5641 ctggatggcg cagtcctgaa aagtaaaaga gcaacgattc cggaaggcgc aacccgccag
     5701 cgctcccacc ggcgttggga ccatcccggg gatggacccc aggaattcgc cgtgctgtgt
     5761 ctttccaggg atggggatac aatcaagtag gccatatgta taaggatgca gaggccgggt
     5821 gaagacatcc cggaccgtcc ccgtttccac gatttgacca gcatacatga ccgccacccg
     5881 gtccgtgacc cgggccacga tccccaggtc gtgggtaatg aggatcattc ccatgcggaa
     5941 ttctcgctgc agccctcgca ggagatgaag aatctgggcc tgaatggtga cgtctagggc
     6001 cgtggtcggc tcatcggcga tgatcaattc aggatcgcac ataagggcca tggcgatcat
     6061 cacccgctgc cggagacctc cagagagctg gtgtgggtac tgtcccagcc ggctcttggc
     6121 cgcagagatg cccaccttct ccaggaggaa tacggctcgc ctccgggcct cggaccggct
     6181 taccctgcgg tggcggatca tgacctccat gagctgattg ccgatggtgt aggaagggtt
     6241 cagcgaggtc atgggttcct ggaagatcat ggacatgcgg tccccgcgga tgtcggccat
     6301 ctcccgctcc ttcagctcta cgaggtcctt acccgagaag gccaggaggg acgccttccg
     6361 aatcgccgat ttgggaagga ggttcatgag ggccagggca gtcatggatt ttccgcaccc
     6421 cgattccccc accaacccca gggtttcccc tttgccaact gtgaaatcga ttccccgcac
     6481 tgcatgcaag tcgccggtga caatgggaat gtcgacgtgg aggttttcaa cttcgagcaa
     6541 agagctcata ataaaaatgc ctaaaattcg aaatgccaaa agcaaaaaaa tatgtctctc
     6601 gcagagctca cagagcgcgc agagatcatg catcaaagaa tcaaattctt tttcttgtta
     6661 aatcattttc ccctcagcgt tctctgcggc ctcttcgaga gatttttttg ctgttggctt
     6721 ttcctttcat accttgaaac tcgaaacccg agactcgaaa ccgtctttta tcgattctcc
     6781 ggcgcggtca cgtcgcgaat tccatccccc aaaaggttga tcgccaggac cagaacaaac
     6841 aaggccaccc ccggaatcgt gatcagccat ggttcgaaga aaagaaagtc cttgccctct
     6901 gagaccatga gcccccagga aggagtgggc ggctgcaccc ccagccccaa aaaggacagg
     6961 gctgcttcca aaagaatagc gttggccatc tccagcgtgg ccactaccac caaattgttc
     7021 acgatgttgg gcatgacttc ggaaagcagg atccgggtgg tggagcaccc caccgcctcg
     7081 gcggccggta tgtaatcctg attgcgaatc tgctgggtgg cgctgcgcat gaccacggcg
     7141 taccggttcc acagcaatag acccagaacg atgatgacca ccttaaggga tcctccaacg
     7201 atgcccacca cggccaccgc caccagcaca aaggggatac tgaggcggag ggtaataata
     7261 aaggtgatca ccatatccac ccgacccccg aaatagccgg ccaccacccc cagggcggtg
     7321 ccaatcaccc cggagatcag ggcaacgcag aagccgatga ggagtgagac ctgcgcccca
     7381 tacagaaggc ggctcaaata gtcgcggcct acatggtctg ttccgaaagg atggttccac
     7441 ttgccctccc catgccagaa gggctttgac agacgtgttg tcaggctttg gacatacggg
     7501 tcatgaggag agagaagggg ggccagcagg gcaaagagga tgatgaccgc caggatgatc
     7561 cctccgatca tcaatccttt gtgcccgaag atgcgacggc gcaggagccg cccgggcgag
     7621 gggccgagga tttcttcgac gggtgggatg ggacggattt ccatttcagg tcacccggat
     7681 ccgcggatcg agccagccgt tcaggaggtc ggccagcagg gtgagaacga tgtagatgag
     7741 ggcaaagacc aggatcaccg cctgcaccgt ggggaaatca gaccgtttga tggactccca
     7801 ggccaggtat cccatgccct ggatggcaaa gaccgtctca accacaatgg acccccccaa
     7861 catgaagccg aattgcaccg cagatacgct gaccacgggg atgaccgcgt tgcggagggc
     7921 atgcttgaag aggatcgatc cgggaagcaa acctttggcc cgggccgtgc ggatgtaatc
     7981 ggaggccagg acttccatca tccccgcccg ggtgagccgc atgatggccg gcatggcgta
     8041 atagcccagg acaatggtgg gcatgacgaa atattcccag ccctccgttc ccgagggggg
     8101 aaggagcggg agccagacgc tcaaaacgac aatgagcatg aggccgagcc agaagctcgg
     8161 catggcctgc cccatgacgg aaaggaaaag ggcggtgcgg tcgacccaac tattgggacg
     8221 gatagcagcg atcaccccta gggggatgga aaaaaccagg gcgaagacga tggcggaaga
     8281 tcccagaatc agggtggcgg gaatccgggc ggccatgatc tggctcacgg gcagtcggaa
     8341 atagtagctc atcccgaaat cccaccgcag ggcttttccg acccaggtca ggtactggac
     8401 atgaatgggc cggtcgaacc cgtagatctt ccggatgttg gcgatgtctg cgtcagtagc
     8461 ggcttccccg ccgatggccg acgccggatc gcccgagagg tagagcaggc caaagctgag
     8521 cgccgttacg accagcgtca ccgccacggc cagggcaagt ctcttcaggg tgtagacgag
     8581 cattctgctc ccccgtcggt tccattgccg ggaggaagcc cggtccctcc aagggaccgg
     8641 ctcctcagtt gcattccgtc attctatttc caggacgcgt tgaagaaacg gggaatctca
     8701 tccggctggg cggaaaaatt caagtccttc gtataggcgt aattcatatt cgaggaccac
     8761 agaggaatgc agtaggcctg ctcggcgatt cgctgcaaag ccaatttgta gttcttcttg
     8821 cgcacctccg ggtcgatgga ggtgtcggcg atctccagcc acttgatcac ctccgggtcc
     8881 cgggcgttgt cgtcggtcgt gcccccgaag aagtttccgg tgaaggcgga ggcgtcatgg
     8941 atggagaagg acccccagtt gttcatggcc atgtccactt cgcccacccg gatcttttcg
     9001 cgggtggcgg cgtaggtcat aaactggatc ttcgtcttga tccccacggc attcaggtac
     9061 cccatgatgg cttccatctc gggccggttc cgccagccgt aaaactcgat ctcaaagccg
     9121 ttggggtacc ctgcttcctc caggagcttt ttggccttgg ccgggtcata ctcgtatttg
     9181 accacgtcgt cggtgcagcc gaactgactt ggatagcagg ccgagtggag gagctgagag
     9241 gctcctttta ccagggtttt ctggatggtg gctttgtcca cggcgtggcc gatggcctgc
     9301 cggaccttca gtttgttgac cggggagttc cgcccttttt tattggcggc gtcgaagtgc
     9361 aggaaaaaga ccctcatggt gtcggaggct tccacggtga tcttgggcat cttggccatc
     9421 ttctccgcct ggtcctgtgg tacccgccag atccagtctg cgcggccgga cataagctcg
     9481 gcgatctgag tgttcacctc gctgatgaag cggatatgca gtttcccgat gctcggcttg
     9541 cccttggggc tggctgcgaa gtaattctcg ttcttggcca gggtaatgct cttgcccggg
     9601 accacctcca cacccttgta gggccccgtt cccactggtt tgagacccat gccatctggg
     9661 cccaccttgg cgtaatattt gtgggggtag atggccagga ccgcggcgat gtactcggga
     9721 gccgccggga aattggtctt ggttttcagg cggaccttgt attttcccag cttctcgcac
     9781 ctctcgatcc agttggagat gttctggctc ttgattccgt tcttcgggtc ggaaatccag
     9841 ttcagggtgt aaaccacgtc atcggcgtcg aactcgtccc cgttgtgaaa cttcaccccc
     9901 tggcgcaggt cgaactccag ggtcttgccg tccacgtatt tccaggaggt ggccaggttc
     9961 ggtttgtatt cgcccgtctt gaggtcgcgg tagagcaggc tgtcccagag gaggtggcac
    10021 agtaccaccc cccagcggtc ggtgttgaag tagttgtcca ccgaggaaag ctcgatctcg
    10081 tggaggatgt taagcgtatc gttggccttg ccagcatggg ccgcaggggc catgaggatg
    10141 aaagcaaata aggcgattaa gcataaccaa gaaaatttct ttctcatctt cctacctcct
    10201 cttatgaata gatcaaggtg aatccccctc ccccaagggg aaaacgaaag cggaatgaaa
    10261 atgctaaagc aaactactcg aatattgatg atctcgtaaa aagtctctcg gttcgtcacc
    10321 ccggcggaag ccggggtcca ggatatcgtg aaataactgg attccggact tcgccggaat
    10381 gacaaatctt atgaaatctc ggctctttgg gaatccatca atatatggct tatatattca
    10441 cggcagtcct aagccaggaa tgcggccagc ttcttcattc cctctttgat catggagacg
    10501 tcgatccccg aataggcgaa ccttatgtac tgctcggtct ctcccggcag cggccggccg
    10561 aaatgcttgc gggtgcagaa gctcacccct gtctccctca agaccagctt acggaattct
    10621 tccacgtcgc tgactccctt gcgcttcatg gctccggtca cgttggggaa gaggtagaag
    10681 gtcgcctccg gcttgaagca gcggaccccc gggatatcgt tcagcagatc aactagcata
    10741 tctcttcttt ctttaaggat ggatagaatg cccagaggtc cttcgttgct cccctggagg
    10801 ccggctaggg ccccgtactg gatgaagtgg ttggagcagg actcgtcgtt cacgttgatt
    10861 ttggagatat attcgatgaa cttcgccggt cctactgcgg cacccaggcg ccagccagtc
    10921 atggcgaatt tcttgctgaa agtgtagagg atgaacgtgc gctcggccat gccgggaaag
    10981 ctggcgatgg acctaggtct gccgctgtaa cggatttcga agtaggcttc atcggctagg
    11041 acgaagagat cgttcttcac cgccagggcg gccagttctt ccatctcctc cggcggggac
    11101 gaagctccgg tagggttctg gtagttgttg taaatcagga ttttggcccc cgcctggatc
    11161 ccttttcgga tggcatccat atccaggatg aaaccgtcac gggcttcctt gtagccatag
    11221 ggaaggccca cgccgccatg gaattcgatc tgcgattcgt agatcgggta acccgggttg
    11281 gggtagagga ctttgtcgcc gggattcatg gccgtaagga tgaatttccc gatggtgggc
    11341 ttgccccccg gctggatgga gacgttgtcc gccgtgagtt tcagcttgcg ctgttccccc
    11401 acgtccttgg ctacggcctc ccgcaggtcc gggatcccgg ccgtggggca atagccggtc
    11461 ttcccttttt tcatggcctc gatggctgcc tccatcacgt tggagggggt gatgatgttc
    11521 atatcgccca gatgaaaggg atagattttt aaccccttct gggcgagctg cgaggcttcc
    11581 agggaaacgg caaaggccgt ctcggtcccg atacggctca ttcgctcggc gatgggtacg
    11641 gtcatggctt cgactccttt ggattcaaag aggattcccc caggaaatcc gcctattgca
    11701 gaccctgctc cgatgaacgt ctacgggcct tctacatata tttcctcctg acccgctccg
    11761 gctcgggaat aaggggatta tagggagaag gtagggcagt gtcaaggatt tcaaatccaa
    11821 gggtttactg gttgctcctt ccttgtttct cgtttaaggc gaattgaggg cattgatcag
    11881 aaggtaagga gtaaccagca atgcattgaa atccgaactc catttggcgt ttgacaccga
    11941 cttcccccta tcgtatattt cccttatttg aggaataaga aaggaaaagg agcgcaaaat
    12001 ggcactcaaa ttcggaccga gaagaggaac gtacacagac atctccagga agatgaaagg
    12061 ggggggactt tcctcggacg aaaagaaggt gttcgaagat tttgacctgg tttaccgttc
    12121 cttgtgcgcc cttctctaca attacgttcc ggcttccggc catcccggag gatcgatctc
    12181 ctcgggacgg ttcgttgaag ggattctctt tggggccatg gactacgacg tctccaaccc
    12241 cgaccgggaa gatgccgatc tcatatctta tgcggccggg cataaggccc tcggactcta
    12301 ttccatgtgg gccctacgga acgaggtgat ccggatcagc gcgcctcatc ttcttcccaa
    12361 agaggttcgc taccagctcc ggttggaaga cctgctgggt ttccggcgca acccgatcac
    12421 ccaaacaccc ctatattcga ggtttggctc caagcccctc gacggccacc cgactccggc
    12481 aacccccttc ctgcgtctct ccactggagc ctcgggggtg ggagcggcct cttctttcgg
    12541 tctggccttc ggggccatgg attactatgg aaaggatgcc ccccgcgtgc acatcgtaga
    12601 gggagaagga ggaatgaccc ccgggcgcgt cagtgaagcc atggctgccg cgggtacggc
    12661 ttgcctgaag aacatcatcc ttcacgtgga ctggaaccag gcctccatcg actccaaccg
    12721 ggtctgccgg gaaggtaacg acccgggcga ttacgttcaa tggaatcccc ttgagtttgc
    12781 ctacctccac gactggaacg tcatctgggt acctgacggt ctggatttcg ggcaggtctt
    12841 cgctgcccaa caagctgcgc ttcggatgag aaacactcag cccacggcca tcgtctaccg
    12901 gacccaaaag ggatggcagt acggaatcga ggggagagcc tcccacggag ccggccatgg
    12961 cctctgtacc gacggctttt atcaggccct gaagccgctc accgacctca tgaagggcga
    13021 tctgccccgc tgccagggcg gcagccagcg ctgccaggcg ggcaaggaca aggcggtgat
    13081 cgaagagtgt ttctgggaag ggctccagct gattcgcagc accctggaaa aaaaccggcc
    13141 catggtcgaa cccctggcgc agagactgat caacgcccgt ttgcggctga accgcagagg
    13201 ccgcataccg cgcgcgaacg gcccggtcat cgaatccgtg ttcgaagcgg ccaagggaac
    13261 cgcagggacc attcctcaga acctggtcct ccagcccgga aagaacacca ccttgcgtgg
    13321 tgaactgggc cgggttctgc actactacaa caaggccagc aatggggcca ttctcaccgc
    13381 agccgccgac cttctcggtt cgaccagcgt caacaccgcg gcccaggggt ttccggaagg
    13441 ctacttcaac gcttccacca acccgggcgc aaggcttctt tccatcggcg gcatctgcga
    13501 ggacgccatg tccggcattt tggccggaat ctccaccttc ggccaccacg tgggtgtcgg
    13561 ctcctcctac agcgccttca tcgcccccct tggccatatt tccgcccgcc tgcactccat
    13621 cggcaaccag tcccgccagg ccatcgctca agagccttac cggcccatga tcctggtttg
    13681 cgcccatgcg ggggtgaaga ccggcgagga cgggcctacc cacgccgacc cccaggcgct
    13741 ccagctcctt caggaaaatt tcccccttgg aaccctgatc accctgaccc cctgggaccc
    13801 ccaggagatg tggactgtgg tttccgccgc cctggacagg aggccggcag tgatcgcccc
    13861 ctttgtcacc cgccccagtg aaaaagtgat tgaccgccaa gccttgggcc tggccccagc
    13921 gacggccgca gcctccggcc tgtacctatt gcacggtgct cagaaacagc gcgcggggac
    13981 gatcgttctc cagggaagcg aggtgggcta tgcttttgtc gaacacgctc tccccttgtt
    14041 gaagaaagaa gggatagacg tggacgctta ctacgtggcc agcgccgagc tttttaacct
    14101 tcttccccaa ggggagcggg aaaagatatt ccccgaggaa cgcgccagag aggccatggg
    14161 aatcaccggc ttcacccttc ccaccctgga ccgctgggtc ctatccgaac gaggacgcag
    14221 gcactctctc catcctttcc agaagggcca ttatctggga agcggccagg ccgaccaggt
    14281 catggccgag gccggattgg atggggaaag ccagttcaag gccattctcc ggtacgtgag
    14341 agaaagggac tagatttttc ccagggagtg caggaagaag gcggcagagc ccacagccgt
    14401 tttaggacat agagaaaagc acaaatacca gcgtcaggcg aagccgattg ggtctctttg
    14461 cgttgtaaag ccgtctttat tcgtagcccg tatatctctg cgcaatctgc ggataaacaa
    14521 gttttgctat tcggatttat ccttcaggcg gtgatccatc tcttcccttc ctgctcgagc
    14581 aaccggttcg cttcaggggg tccccacgtt cccgcggcat agttgggaaa atttctcggc
    14641 ggttggtttt cccagcagga aatgatcggg tccacggctt cccacatggc ttcgatctga
    14701 tcctgccgga caaagagggt caggtcccct ttcaaacaat cctggatgag tcgctcatag
    14761 gccgggtggg gagcggattg aaaggtctcc ttgtagctga atttcatatc gatgggataa
    14821 atctggttgg gcatgccggg caccttcact ccgaaccgaa gggagatctt ttcttcgggt
    14881 tgaatcgtca ggaggaggac attggcctcc agcatgtcgc aggtccggcc gaagagcctc
    14941 agggggggct ggtcgaactg gatggcgatt tcggtaaccc gcttggccaa ccgtttcccc
    15001 gtccgaacgt aaaaaggaac cccggcccat cgccagttgg caatgtagaa ggaccccgcg
    15061 aaaaaggtgg gggtggcgga gtcgggggac accccttttt cctgccggta ccccggcacc
    15121 tcctttccct ggacctttcc cggcccatac tgcccgatga cgctgaaccg gtccacctgt
    15181 tcttccctca agggatgaag ggaccggaag accttcgtct tctcatcgcg gatgaagtcg
    15241 gcctcgaacc ccaccggggg ttccatagca atcaacccga tgagctggag gatgtggttc
    15301 tggacgatat cgcggatcac cccggcctgt tcgtagaagc ggacccggtt atcgatgcct
    15361 atgtcctcgg ccaccgtgat ctggacattg tcgatgtacc ggcggttcca tagctgttca
    15421 aagatgctgt tagaaaagcg aaagaagatg atattctgta ctgtctcctt tcccagatag
    15481 tggtcgatgc gatagacctg acgttcgtcg aaggcgctgg tcaggaggcg gttgagttgt
    15541 accgcagagg agaagtctct cccgaagggc ttttccacga tgatcttggt gtccagcggc
    15601 cctcggcaaa gtccgcgcgc gttcagcagg ttcaccgccg taggcgtcat ctgggggggg
    15661 atggccatgt agtaaatcac attcttgtga ccctcccggt ccggaaccat gacttggtcc
    15721 agccgggagc agagcctctg ataggtccct tcttcctcca ggttccctgg gaggtaaaac
    15781 aagcggctgc ggaactcggc ccatttctcc tcgctgaaac agacctcgtc ggacccccgg
    15841 agggcccgtt ccatcaaggc ccggtaggaa tcgtcttcca gttttgatcc tcccaagctt
    15901 aggatagaaa aatgctccgg gagctcgttc cctagataga tgtgaaagag ggcggggagg
    15961 agcttcttac ggctcaggtc cccggtcccg ccgaaaatca ccagggtgaa aggctcgacc
    16021 ttgaaactct gcgcggtaat gtcgcaggtc tgcagatact tggtgaccgc gacgtcttcg
    16081 atcctgcttt tctcgaccat tttattctcc ctgctgcgga ttgcaggatt agcctatcaa
    16141 gcctgcgtgg cgcaaggcga aaaaatcgga atttatcgat ccggctcacc tctcaaatct
    16201 gattataaac gatgtggctt gtttcggaga atgaaaaaaa gctccttttc ccctttgggt
    16261 aatattcagg ggaaaaagag aaaagttctt tcccttcctc cagggtctcc ctccggccaa
    16321 aaacctcaac cgatggaaac cattgatcat tgaatggtct tccctctttg gaatgagtct
    16381 tgctaaatga aggagtatta cttcccacta ttgaagggga tggagaggga ctcagatgag
    16441 aaagttttgg atcatcatgt tt
//
LOCUS       DASPRR010000206         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_53444, whole genome shotgun sequence.
ACCESSION   DASPRR010000206 DASPRR010000000
VERSION     DASPRR010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53444"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VEL68_07950"
     CDS             <1..243
                     /locus_tag="VEL68_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXZ35936.1"
                     /translation="MAPPGIDGQKVVKILRDKHELTIAGGQDQAKGKVFRLAHMGYIE
                     QSDIIAGITALELTLKELGYSFELGAGVRAAMEILI"
     gene            321..1631
                     /locus_tag="VEL68_07955"
     CDS             321..1631
                     /locus_tag="VEL68_07955"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXZ35937.1"
                     /translation="MANIVIVGMQWGDEGKGKVVDLLTEYAHVVVRFQGGNNAGHTVV
                     VGQETIILHLIPSGALYRDKTCVIGNGVVVDPQVLLQEIDELRKRGHFLDDRQLLISE
                     DAHLIMPYHRRIDVARERMKGEGKIGTTGRGIGPAYEDKVAREGIRFGDLLDDQIFRG
                     KLEAILSTKNHYLDACLKDQGFDLEAMFQEYRGYGQRLKKFAGNISLFIDRQIKQGRQ
                     ILFEGAQGTHLDVDHGTYPFVTSSNTVAGGACTGAGVGPTRITEVIGVSKAYTTRVGG
                     GPFPTELNDSVGEKIRERGREFGATTGRPRRCGWLDIPLLKDAIRLNGITGIALTKMD
                     VLSEFETIRICTAYQHRGRRIEEVPSQVQALKECEPVFEDHPGWKAELRNFRSFQDLP
                     SKAQGYVRRIEELTGTEVTLVSVGEKRVETILRRNPFQQGPRLS"
     gene            1810..1885
                     /locus_tag="VEL68_07960"
     tRNA            1810..1885
                     /locus_tag="VEL68_07960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1843..1845,aa:Lys,seq:ttt)
     gene            2291..>2979
                     /locus_tag="VEL68_07965"
     CDS             2291..>2979
                     /locus_tag="VEL68_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ35938.1"
                     /translation="MTVPKILSRGFLLSFLAQFTFSLVFCILIPAIPIYLLRFGAKEG
                     EIGLLIGIFSVSSLILRPFVGKALLTIPERDFMIAGTLLYVVSCLAYLIAPPFWPLLI
                     VRAVHGIGLAFFATASFTLLASMLPEAHRGRLISYYFLSFNLAFALGPYLGMLLINRF
                     KFSILFLVCTGLSLCSFYLTLKLGKRESIASDRESRKTQSFLSREALPPAIIAFMLNF
                     IWGSIAAFIPL"
BASE COUNT          647 a          794 c          814 g          724 t
ORIGIN      
        1 atggccccgc cgggaatcga cgggcagaag gtggtgaaaa tcctccgcga taagcacgaa
       61 ctcacgattg ccggaggcca ggatcaggcc aagggaaagg tcttccgcct ggcccatatg
      121 gggtacatcg aacagtcgga catcatcgcc ggcattactg ccctggaatt gaccttgaag
      181 gaactgggat actcttttga attgggggca ggggtgcggg cggcgatgga gatattgatt
      241 tgacagggaa aggggagaat ttctttcacg ctatgcgcta tgctctcttc gctatgcgat
      301 ttttcgcgta aaggattgtt atggccaaca tcgtgattgt cggaatgcaa tggggcgatg
      361 agggcaaagg gaaggtcgtc gatcttctga ccgaatatgc ccacgtggtg gtccgcttcc
      421 agggggggaa caacgcgggg cacaccgtgg tcgtgggcca ggagacgatc atccttcact
      481 tgatcccttc gggtgccctg taccgggaca agacctgcgt gatcggaaac ggggtcgtgg
      541 tcgatcccca ggtccttctg caggagatcg acgagctcag gaaacggggc catttcctgg
      601 acgaccggca gctcctgatc agcgaggacg cccatctgat catgccctac caccgaagaa
      661 tcgatgtggc ccgggagcgg atgaagggag agggcaagat cgggactacc gggaggggga
      721 tcgggcccgc ctatgaagac aaagtggccc gggaaggaat ccgcttcgga gacctcctgg
      781 atgatcaaat tttcagggga aaattggagg ctatcctctc caccaagaac cactacctgg
      841 atgcctgcct gaaagaccag ggtttcgacc tggaggccat gttccaggaa taccgtgggt
      901 atggccagcg actgaaaaaa ttcgccggca acatctccct ctttatcgac cgccagataa
      961 aacagggccg tcagattctc tttgaaggag cccagggaac ccacctggat gtggaccacg
     1021 ggacctaccc cttcgtgacc tcctccaaca cggttgccgg gggagcctgc accggggccg
     1081 gggtggggcc caccaggatc accgaagtca tcggcgtctc caaggcctat accacccggg
     1141 tgggaggagg tcccttccca accgagttga acgactccgt cggggaaaag atccgggaaa
     1201 ggggccggga gttcggggcc accaccggaa ggccccgccg ctgcgggtgg ctggatattc
     1261 ccctgctcaa ggacgcgatc cggctgaacg gcatcaccgg aatcgccctg accaagatgg
     1321 atgttctcag cgagtttgaa accatccgca tctgcaccgc ctatcagcac cgcggccggc
     1381 ggatcgagga ggttccctcg caagtccagg ctttgaagga gtgcgaaccc gtcttcgaag
     1441 accatccggg gtggaaggcg gagctcagaa acttccggag cttccaggac ctcccctcaa
     1501 aggcgcaggg ttatgttcgg cggatcgagg agctgaccgg gaccgaggtt acccttgtct
     1561 ccgtggggga aaagcgggta gagaccatcc tgcgccgcaa ccccttccag caaggccccc
     1621 gcctttcata aaacacttga tcagcggagg aaatgcgttt agaatttatt catagacgcg
     1681 gatttccggg gactctcgca aataaataga attcaggagt caatagccaa gatgcttcca
     1741 cttctgaatt ctgttttttg gtttttttat cccagaaatc tgcgttcatc tgcgtcccaa
     1801 aaaggtttgg agccgttagc tcagtcggta gagcacctga cttttaatca ggtggtcgtg
     1861 ggttcgagcc ccgcacggct caccataatt tcaaggggtt agataaggtt tctagcccct
     1921 cttttatttt ttggggcttc gtgtaggcgc caaggaagac caattcccct tcaatgggag
     1981 tctggcttct tctatctgtt catggccgga gggcgctcgt ggagcgctga tcgtttaggg
     2041 gtcggaacga tctttaaggt agctgcggct cggcccaggc atgcttatgc tccaaaagct
     2101 tcttcgcgaa tttgccagtt gagggatatt gtcaacagct atagaccttt acggcaacca
     2161 acatttggaa atgatcatga taatatattc aattcgggat ctttttccag agccagaagt
     2221 acggcacgat ctgtagcttt tacaccttca gtatgatcca atttcctatt cccagggcca
     2281 gaaacacgag atgaccgttc caaaaatatt aagccgcggt ttccttctca gtttcctcgc
     2341 tcaatttact ttctcattgg tcttctgcat tcttatcccg gccattccaa tttatctctt
     2401 acgattcgga gccaaagagg gagaaattgg cctgcttatc ggaattttca gtgtctcctc
     2461 attgatcctt cgcccctttg tagggaaagc tcttttgact atccctgaaa gagacttcat
     2521 gatcgctggg acgcttctct acgtagtctc ctgcctggcc tatcttatag cccctccttt
     2581 ctggcccctc ctgatcgtgc gggccgttca tgggattggg ttggcctttt ttgcgaccgc
     2641 ctcctttacc ttgttagcaa gcatgcttcc agaagctcat cgtggtcggc tgatcagtta
     2701 ttatttttta tctttcaacc tggcttttgc tcttgggccg tacctcggca tgctcctcat
     2761 caatcgcttt aaattttcta ttcttttcct ggtttgtacg ggcttatcgt tatgctcctt
     2821 ctatctgacg ttgaaattgg gaaagaggga atccatcgca tccgaccggg aatccaggaa
     2881 aacccaatcc ttcctgagtc gggaagcgct tccgccggcc atcatcgctt tcatgcttaa
     2941 ctttatctgg ggaagtatag ctgcttttat ccctctata
//
LOCUS       DASPRR010000207         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1016352, whole genome shotgun sequence.
ACCESSION   DASPRR010000207 DASPRR010000000
VERSION     DASPRR010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016352"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VEL68_07970"
     CDS             <1..604
                     /locus_tag="VEL68_07970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ35939.1"
                     /translation="CGIGRLADRLTQKADEVVGVELSDGVDRAWELFHSNPRVHFIQG
                     NLYTLPLRPRTFDYVYSKGVLQYVSNPREAFRQVVRSVRPGGGVSITLYPPLPPFFFF
                     LNRSIRALTLRLPLGWVQALSFLAVPFLSTAWKASRVEMRRVSWRDRGHMIFNWLAAD
                     YQNFHSTEEVVSWYREEGFGRICTSPVPVGVFGRWEGHVT"
     gene            594..1586
                     /locus_tag="VEL68_07975"
     CDS             594..1586
                     /locus_tag="VEL68_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HXZ35940.1"
                     /translation="MSLKSSLGLRLLGFFGVALILGFLVRELWSNWTALQAYSWKVKP
                     GLALFSFAALTGSLFLLPWGLREVLALLGHRLPYSRVSKILFSSLLAKYLPGGWWALL
                     GRAHFYRQEGLPLAQASLAVLMETVLVVTSGILTFSLFFPGHSAFLGDWQKGLFALIG
                     ASCLIFIHPYFLNLFLFFTAKVFKKPGAPLSFSYWSMAYPFAVFFFFWVGMGASFWLL
                     AASLTDVELSLLPQMISAFTLSWAVGFFSFVTPGGLGVREGALALLLKPFFPLYAAAA
                     LALLSRVWWLAGEVLAWVISFLWARPSRGEVRAFSKTESTHAGLPSMPKEKRDY"
     gene            1589..2236
                     /locus_tag="VEL68_07980"
     CDS             1589..2236
                     /locus_tag="VEL68_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35941.1"
                     /translation="MRGRWVLGSILLGVMGLALFYGKVFYHSGEEYRKGEDAFRQGDF
                     NKALLHYDRAIHWYTPWSSSVERSILRLWEMGEKLEEKGDQKTALEVYWALRSSLYGV
                     RSFYTPQMEWIEKANERIASIWAGQEPVPAEEKKITPTERKERYLAALKKDWAPKVGW
                     AVATEVGFFGWVVSALVFIFTFLRKGGGLARGKALFWGGCILIFYALWVIGMVRA"
     gene            2240..3205
                     /locus_tag="VEL68_07985"
     CDS             2240..3205
                     /locus_tag="VEL68_07985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ35942.1"
                     /translation="MEEGICTICRTPTRSTPYLSAQAEGTLFHYQRCSVCGLVFLNPR
                     PDPEQLLRYYGRDYYGGGERKFTRGIEALRLLFALRRVYRLQRYLPRAGRALDIGCGQ
                     GAFLQLLRRQGWAVQGTELMEEPARRARQAGIPVFLGEIQEGQLGEGSLDLVTLWHVI
                     EHLRDPAGMLRRISLMVGKKGLVALSTPNVESTQARVFRERWFHLDPPRHLYLFSPET
                     LHHLMAQAGFRMIYLNHFSLEQNPYGWVQSALNRMAFPGGDLYSFLKSQPGRKRPENS
                     PFLAKMLLLSAALLLPSILISLLMAWRGEGGTLEAYFIKDEAGCG"
BASE COUNT          616 a          945 c          983 g          848 t
ORIGIN      
        1 ctgcgggatc ggtcggctcg ccgaccgcct cacccagaag gcggacgagg tagtgggggt
       61 ggaattaagc gatggagtgg accgggcctg ggaactcttc cattccaatc cgcgggttca
      121 cttcatccag ggaaacctct atacccttcc ccttcgtccg cggacttttg actacgtcta
      181 tagcaaagga gttctccagt atgtatccaa cccccgggag gccttccgtc aggtagtccg
      241 ctcggtccgt ccagggggcg gggtttccat cactctttat cccccgctgc ctccgttctt
      301 cttctttctc aatcggtcga ttcgggcgct gaccctgcgg ttgcccttgg gatgggttca
      361 ggccctgtcc ttcctggctg tccccttcct ctccacggcc tggaaggcca gccgggtgga
      421 aatgcggcga gtctcatggc gggaccgagg acacatgatc ttcaactggc tggcggcgga
      481 ttaccaaaat tttcattcca ccgaagaagt cgtatcctgg taccgggagg aggggtttgg
      541 gaggatctgc acctccccgg ttcctgtcgg ggtattcggt cgatgggagg ggcatgtcac
      601 ttaaatcgtc tctggggctg cgactgctgg gattcttcgg agtggccctc atcctgggtt
      661 tcctcgtacg ggaactctgg tccaactgga ccgcccttca agcctactca tggaaggtga
      721 agcccggact ggccttgttc tcttttgccg cccttaccgg ctccctattt cttcttccct
      781 ggggattgcg agaagttttg gctttactcg ggcatcgtct gccctattcc cgggtcagca
      841 aaatcctctt ctcctccctc ctggccaagt atcttcccgg cggatggtgg gccctgctgg
      901 gccgggctca tttttatcgc caagaagggt tacccctggc ccaggcctca ttggccgtgc
      961 tcatggaaac ggtcctggtg gtaacctctg ggatcctgac cttctccctt ttctttcccg
     1021 gtcattccgc tttcttgggg gactggcaga aagggttgtt tgccttgata ggggccagct
     1081 gcctgatttt catccatcct tattttctta acctcttcct ctttttcacc gcaaaggtct
     1141 ttaaaaagcc gggggccccc ctgagttttt cttactggag catggcatat ccctttgccg
     1201 tctttttctt tttctgggta gggatgggag ccagtttctg gctgctggcc gcttccctga
     1261 cggatgtgga gctgtccctg cttccgcaga tgatcagcgc ctttaccctt tcctgggcag
     1321 ttggattttt tagctttgta accccgggag gattaggggt tcgcgaaggg gctctggctc
     1381 ttcttttgaa gccctttttc cccctctatg cagcggcagc cctggccctg ttatcccggg
     1441 tttggtggtt ggcgggggag gtcctggcct gggtcatttc ttttctttgg gcacgcccga
     1501 gccgggggga ggttcgagcc ttctcgaaga ccgaaagtac ccatgcaggg cttccatcca
     1561 tgcctaagga gaagagggat tattgaacat gagaggacgc tgggttctgg gaagcatcct
     1621 tttgggagta atgggcttgg ctctttttta cggaaaggtt ttctaccatt ccggggagga
     1681 atacaggaaa ggggaagatg ccttccgcca gggagatttc aataaagccc tcctccatta
     1741 tgaccgggcg atccactggt atacgccctg gagttcttcc gtggagcgct ctatcctccg
     1801 cctctgggag atgggagaaa aactggagga aaaaggcgat caaaaaaccg ccctggaggt
     1861 ctactgggct ttacgaagca gcctttacgg ggtgcggagc ttctatactc cccagatgga
     1921 atggatcgaa aaggccaatg agcggattgc ctccatctgg gcggggcagg aacccgtccc
     1981 cgccgaagaa aagaaaatca ccccaacgga gagaaaggag cgatacttgg ccgccctcaa
     2041 gaaagactgg gcccccaagg tcggctgggc cgtggccaca gaggttgggt tctttgggtg
     2101 ggttgtttca gccctcgtgt tcatctttac ctttctgcgc aagggaggcg ggctggccag
     2161 gggtaaggcc ttgttctggg gggggtgcat ccttatcttc tacgcccttt gggtgatcgg
     2221 catggtccgg gcatgaacga tggaagaagg catctgcacc atatgtcgta ctcccacgag
     2281 gtctacccct tatctttcgg cccaagccga aggaaccctg tttcattatc agcgatgctc
     2341 cgtttgcgga ctggtttttt tgaacccccg gccggacccg gagcagctcc tccggtatta
     2401 cggccgggat tactatggag ggggagagcg aaaattcacc agggggattg aagccctgcg
     2461 gcttcttttt gccctaaggc gggtttaccg cctgcaaaga tacctgcctc gggcgggccg
     2521 agctctggac atcggctgcg ggcaaggtgc ctttctgcaa cttcttaggc gacaagggtg
     2581 ggcggtgcag ggaacagagc taatggaaga gcctgcccgc cgagccaggc aggcgggaat
     2641 tccagttttc ctgggggaga ttcaggaagg acagctcggg gaagggagcc tagacctggt
     2701 tactctatgg catgtaatcg agcatcttag ggaccccgcg ggtatgttgc gccgaatttc
     2761 actcatggtg ggcaagaagg gcctggtagc cctatccact cccaacgtgg agagtacgca
     2821 ggcccgggtc ttccgggaga gatggtttca cctggacccc ccacgccatc tctacctctt
     2881 ctctccggaa actctccacc atctgatggc ccaggccgga tttcggatga tttacctgaa
     2941 ccatttttcc ctggagcaaa acccttacgg gtgggtccag agcgccctta atcggatggc
     3001 ctttcccgga ggggacctgt attctttcct gaagagtcaa cccgggagga agaggccgga
     3061 gaactcgccc tttttggcca agatgctcct attatccgct gctctcctcc tcccctccat
     3121 cctcatatcc ttgctcatgg cctggagggg ggagggtggg accctggaag cttactttat
     3181 caaggatgag gctggatgcg gataaattgg atcatgaaag atgaagttat gcccagcctg
     3241 atcccggggg agaagaaata tccttcctgg atgacctcgg tcctcctctt cgctctattc
     3301 ctgatcaccc tggcggttta ctacccttct ctcggcggcg gcttcctgaa tatcgacgac
     3361 cagtattata tcgacgagaa tgaattcatc cg
//
LOCUS       DASPRR010000208         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1662148, whole genome shotgun sequence.
ACCESSION   DASPRR010000208 DASPRR010000000
VERSION     DASPRR010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662148"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VEL68_07990"
     CDS             complement(<1..916)
                     /locus_tag="VEL68_07990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ35943.1"
                     /translation="MKRANPVREDNPDSPLVPGAPVRLLSRIPASRLIADWMNSLEID
                     IGGLLRGIREILLFRCQASQVDFFMPPEAAGTPGFYEELRKYDWYYMADKWEFTEALR
                     DLAGKQRILEVGSGTGLFVERAGKELKGAWVQGIEFNESAVSQAREKNLPVVQMDLDE
                     LGRRGETFDAVCGFQVLEHVHHARELLTAMIKVLEPGGVLILSVPNRDSFLRHQYNLL
                     DMPPHHMTRWNAEALSYLSRIFPLRLLALRYEHLAPYHISGYVAAYGEYFGAKVPFLR
                     RRLTKERLVRLADFIQQRGLNRFLRGQSL"
     gene            complement(956..2326)
                     /locus_tag="VEL68_07995"
     CDS             complement(956..2326)
                     /locus_tag="VEL68_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35944.1"
                     /translation="MNDTVAVSVRNLGKHYMLYEKPRDRLKQSLLRHFGKRYGHAFWA
                     LRGASFDVRRGEILGVIGRNGSGKSTLLQMIAGTLAPSEGEIHVRGRVAALLELGSGF
                     HPEYTGRENIFMNGAILGFSRREMEGRLAEIVSFAEIGEFIDQPAKTYSSGMFVRLAF
                     AVQACVDPDVLIVDEALSVGDFFFQMKCHARMEELMNRKTAIILVSHDMSMIEYYSTR
                     TLLLDKGRCLFLGQPNQAVEEYYRVERPFQRLSQPGEGPAIQCEGEKPASGQESIPDW
                     PKPEAFLDLSRAVVIGEEDIARCTGIALCNDRGQPCSTFQIGEEANFYFEFEILRDIQ
                     VPIGGVVITNRMNINVHGKNSLQINLPAPTGVRRGSRVRFRQRMEMSITPGEYTFAVG
                     FATMNPEVYARIEEMDYRQLEPQLKTIMRVRQAGIFSVTLRTSGQSLPFYGYADLKGD
                     FALAVL"
     gene            complement(2323..3204)
                     /locus_tag="VEL68_08000"
     CDS             complement(2323..3204)
                     /locus_tag="VEL68_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35945.1"
                     /translation="MVPRADKIIIAATEKDRNPFQRYLRTFFDVAGLVRNLAGHGELI
                     QIMAWRDFTARFRGSFGGLFWSLFHPLVMMVIYTVVFSLFLKLRFSTDASPFTFSVYL
                     LCGLLPWSAISEGLQVSKDVIRSNVNFVKRVVFPLEILPLNAALTSSIQQIFGFFLLI
                     PLAWALTGSLYWTLFFVPVIIFLQLLLAIGLNWMAAGLAVYLPDFSQFLNLLLMVWMF
                     LTPIFYPEDIVPPQALFIFRLNPMAHLVRMYRGAFMTGELPGLESFLGTAFFCAFVFL
                     LGYFWFVHAKKGFADVL"
     gene            complement(3180..4118)
                     /locus_tag="VEL68_08005"
     CDS             complement(3180..4118)
                     /locus_tag="VEL68_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HXZ35946.1"
                     /translation="MSFWSDHRFVVTGGSGFLGSHLVAWMKEQGANLVFVPKSTEYNL
                     VKEEAIIRLYKAARPDIVIHLAAVVGGIGANRENPGRFFYENLMMGAQLMEQARLFGV
                     GKFVAVGTICSYPKFTPVPFKEENLWDGYPEETNAPYGLAKKMMLVQSQAYRQQYGFH
                     SIFLLPVNLYGPGDNFDPASSHVIPALIKKCMDAIRNREDEIVVWGTGKATREFLYVG
                     DAAEAIALATEKYDKEDPVNIGGGFEISIKDLAELIVEITGFRGRIVWDPAKPDGQPR
                     RMLDTSRAFREFGFKAKTHFREGLKKTIEWYREQIR"
BASE COUNT          991 a         1260 c         1223 g          866 t
ORIGIN      
        1 acaggctttg gccccggagg aaccggttca gccctctctg ctggatgaaa tcagccagcc
       61 ggaccaaccg ctccttggta agacgccgtc ggaggaatgg gactttggcg ccaaagtatt
      121 ctccgtaggc cgccacatac cccgagatgt gatatggagc aagatgttca taacgaaggg
      181 ccagaagtcg taatggaaat atacgggaca aatacgacag ggcttctgcg ttccaacggg
      241 tcatatgatg gggtggcatg tccagaaggt tgtactggtg ccggagaaaa ctgtcgcggt
      301 tgggcacgct cagaatgagt acccctcccg gctccagaac cttgatcatg gccgtcagga
      361 gttcccgagc atgatggaca tgctccagca cctgaaaacc gcagacggca tcaaaggttt
      421 cgccccggcg gcccagttca tcgaggtcca tctgcaccac gggcaagttt ttctcccggg
      481 cctggctgac ggcgctctca ttgaattcga tcccctgaac ccaggccccc ttcaactcct
      541 tccccgccct ttccacgaaa agaccagtcc cgcttcccac ctccaggatt cgctgcttgc
      601 cggccaggtc cctgagggcc tcggtgaatt cccatttgtc ggccatgtaa taccagtcat
      661 acttccgcaa ttcttcatag aaccccggag tgcccgcggc ttccggaggc atgaaaaagt
      721 caacctggct ggcctggcag cgaaaaagga gaatttcccg gatgcctctc agcagcccgc
      781 cgatatcgat ttccagggag ttcatccagt cggcgatgag tcgagatgcc ggaattcggc
      841 tcagcagccg aaccggagcc ccgggaacca gggggctatc cgggttatct tccctcaccg
      901 ggttcgctct tttcattcgc catggccgac caccggcgcc atcggggatc gcgccctaca
      961 gtaccgccaa ggcaaaatct cccttgaggt cggcgtagcc gtaaaagggg aggctctgcc
     1021 cgctggtccg taaggtgacc gagaagatgc ccgcctggcg gacccgcata atggttttga
     1081 gctgaggctc gagttgacgg taatccatct cctcgatccg ggcatacact tcggggttca
     1141 tggtagcgaa acccaccgcg aaggtatatt cgccgggagt gatggacatc tccattctct
     1201 gtcggaagcg caccctggac cctctcctaa ctcccgtagg ggccggaagg ttgatttgga
     1261 gcgagttttt accgtggacg ttgatattca tccggttggt aataaccact ccgccgatgg
     1321 gaacctggat gtcccgcagg atttcgaatt cgaaatagaa gttggcctcc tccccgatct
     1381 ggaacgtgct gcaaggctgt ccccggtcat tgcagagagc gattcccgtg caccgggcga
     1441 tatcttcttc tccgatcacc acagccctgg agaggtccag gaaagcttcc ggtttcggcc
     1501 aatcgggaat cgattcctgg cctgaggcgg gcttctcccc ctcgcactga atggccggac
     1561 cctcccccgg ttgggagagt cgctgaaatg gccgctcgac ccggtaatat tcctccaccg
     1621 cctgattcgg ttggcccaga aagagacatc tacccttgtc cagcagcagc gtccgggtgc
     1681 tgtagtattc gatcatcgac atgtcgtgag aaaccaggat gatcgcggtt tttcggttca
     1741 tcagctcttc catccgggca tgacacttca tctggaaaaa gaagtccccc acgcttaggg
     1801 cctcatcgac gattaatacg tccgggtcga cgcaggcctg cacggcaaaa gccagacgga
     1861 cgaacatgcc gctggaatag gtttttgccg gctggtcgat gaactccccg atctccgcga
     1921 aggagacgat ctccgccaat cggccttcca tctctcggcg ggaaaaaccc agaatggccc
     1981 cgttcatgaa tatgttttcc cggccggtat actccggatg aaacccgctt cccaattcaa
     2041 gcagggcggc aacccggccc cggacgtgga tttccccctc cgagggggcc agcgtgccgg
     2101 caatcatttg cagcagggtg ctcttgccgc tgccgttccg tccgatcacc cccaggattt
     2161 ctccgcggcg gacatcgaaa ctggcgccgc gcaaagccca gaaagcatgc ccgtaccttt
     2221 tgccgaagtg gcggagcagg gattgcttga gccggtccct gggtttttca tagagcatgt
     2281 aatgcttgcc caggttccgg acggaaacgg cgactgtgtc gttcatagta cgtcggcaaa
     2341 ccctttttta gcgtgcacaa accaaaaata tcccaggaga aagacgaaag cgcagaaaaa
     2401 ggcggtcccc aagaaggatt ccaggcccgg taattcaccg gtcatgaatg ctccccggta
     2461 catccttacc aggtgggcca tcgggttcag ccggaagatg aataaggcct ggggggggac
     2521 gatgtcttcc gggtaaaaaa tcggcgtcag gaacatccag accatgagca gcaggttcag
     2581 aaattggctg aaatcgggca ggtagaccgc cagaccggcg gccatccaat tcaggccgat
     2641 cgccagcaga agctgcagaa agatgatgac cgggacgaaa aacaaggtcc aatacaggct
     2701 gccggttaat gcccaggcca ggggaatcag caggaaaaat ccgaatatct gctggatcga
     2761 ggaggtcaag gccgcattca ggggtagaat ctccaggggg aagaccaccc gtttcacaaa
     2821 attgacattg gagcggatga cgtccttgga gacctgaagg ccttcactga tggcgctcca
     2881 gggcagaagg ccgcatagga ggtataccga aaaagtgaac gggctggcat ccgtggaaaa
     2941 gcgcagtttc aaaaaaaggg aaaagaccac ggtatagatg accatcatca ccagcggatg
     3001 gaagagagac caaaaaagcc ctccgaagga gccgcggaag cgggcggtga aatcccgcca
     3061 ggccatgatc tgaatgagtt ccccgtggcc ggcgagattc cggaccaaac cggctacgtc
     3121 aaaaaaagtg cgaagatagc gctggaaggg gttgcggtct ttctccgttg cggcgatgat
     3181 tatcttatct gctcgcggta ccattcgatc gttttcttca agccctcccg gaaatgggtt
     3241 ttcgccttga atccgaattc ccggaaggcc cgggaggtgt ccagcatcct gcggggctgt
     3301 ccgtcgggct tggcggggtc ccacacaatc cgaccccgaa agccggtgat ttccacgatc
     3361 agttcggcca ggtccttgat ggaaatctca aagccgccgc cgatgttgac cgggtcttcc
     3421 ttatcatatt tttcggtggc cagggcgatc gcctcggccg cgtcccccac gtacaggaat
     3481 tcccgggtgg ccttccccgt cccccagacg acgatctcat cctcccggtt ccggatggcg
     3541 tccatgcact tcttaatgag agccgggatg acgtgggaag aggcggggtc gaaattgtcc
     3601 cccggcccgt ataggttgac cggaaggaga aaaatggaat ggaagccgta ctgctggcgg
     3661 tacgcctggg attggacgag catcattttt ttggccaggc cgtagggggc gttagtctcc
     3721 tcggggtagc cgtcccacag gttttcctct ttaaagggga ccggggtgaa tttcgggtag
     3781 gaacagatgg tgccaacggc cacgaatttc ccgaccccga agaggcgggc ctgctccatc
     3841 agttgcgcgc ccatcatcag gttttcataa aaaaaacgcc ccgggttttc ccggttagcc
     3901 ccgatccccc ccaccaccgc cgccaggtgg atgacgatat cgggccgggc cgccttataa
     3961 aggcggatga tcgcctcttc cttgaccagg ttatattcgg tgctcttggg gacaaagaca
     4021 agattggctc cctgctcctt catccaggcg actagatggg agcccaggaa ccccgaaccc
     4081 cctgtgacca caaaacggtg atcgctccag aaactcaatc cttcacccct ccggaattcc
     4141 tcaaagggaa catccatcca accccaagat tggactgcat gatgaatatc gacgcaggtc
     4201 ccaagcctta cgcggggtcg aaaaaaagtt tacagatttc ccgtttcatc tgcccataaa
     4261 gataactcaa aacacaccct agggggaagt aaaaaatgcg gaaaagggga tggccaacgg
     4321 gccatgaagg aaacgatatg
//
LOCUS       DASPRR010000209         6386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1340041, whole genome shotgun sequence.
ACCESSION   DASPRR010000209 DASPRR010000000
VERSION     DASPRR010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340041"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VEL68_08010"
     CDS             <1..470
                     /locus_tag="VEL68_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35947.1"
                     /translation="VPSAMAQHQHGGMSGMPGMEGEKKPESALGNEASPNRTFLLQGN
                     VKAAFSIMAMVEHKKMLQEMKMKMEVDPQATHNIAVTLSDTRTNLPITDAVVKMKVID
                     PKGKDQIKPLDFIAGMNQYAGDFTLSGKGRYQVLILFKAGGNKESAGFYYLQK"
     gene            661..840
                     /locus_tag="VEL68_08015"
     CDS             661..840
                     /locus_tag="VEL68_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35948.1"
                     /translation="MENFPAMRALDEFKSPTAMGIFFGGDWWLTEKIYLGGDARLFGE
                     HSGTFSAQHHFEWYN"
     gene            912..1112
                     /locus_tag="VEL68_08020"
     CDS             912..1112
                     /locus_tag="VEL68_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXZ35949.1"
                     /translation="MEKIKIQGMTCQHCVMSVTKALGNLPGVKNLRVDLVKGEAAFEN
                     TQNISRDSLRKAVEDAGYTVVD"
     gene            1213..1677
                     /locus_tag="VEL68_08025"
     CDS             1213..1677
                     /locus_tag="VEL68_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXZ35950.1"
                     /translation="MRLSTKSRYGVRALFDIAYHSKGHPTQIKEISRREGITPRYLEQ
                     IFQKLKKAGMVKSVRGPKGGYYLSRRPEEIVVGDIIRAMEESIEPVHCARTTRDRKKC
                     RRESRCAAQVVWKEAGQRLREYLDSTSLGTMCKIARNIGVGKPLDSRFMDAV"
     regulatory      1740..1854
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2006..3193
                     /locus_tag="VEL68_08030"
     CDS             2006..3193
                     /locus_tag="VEL68_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HXZ35951.1"
                     /translation="MNPHKVNKTIAEINDKIRRGKAVVVTAEEIIDIVEEKGPAKAAL
                     EVDVVTTGTFGPMCSSGAFLNFGHTQPRIKAAKAWLNGVPAYAGVAAVDIYIGVTEPT
                     EDDPLNKVYPGEFKYGGGHVIQDLVAGKPVLLRAEAYGTDCYPRKKLEKEITLKTVQE
                     AFIFNPRNAYQNYNCAVNLSDRTIYTYMGVLRPNAGNANYCSAGQLSPLMNDPLYKTI
                     GIGTRVFLGGGQGFIVWRGTQHNPNVKRGSNQVPRGGAGTLSVLGDMKQMSPEWLVGA
                     SFQGYGCTMIVGIGVPIPLLNEEIAQFTAVKDEDIYTQIIDYSKDYPEGGPTQSLGEV
                     NYKQLKSGRIKFNGKEIPTTPLSSYPKARKIAEVLKGWIQKGEFTLGEPQQLLPSVSP
                     TRL"
     gene            complement(3213..4199)
                     /locus_tag="VEL68_08035"
     CDS             complement(3213..4199)
                     /locus_tag="VEL68_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HXZ35952.1"
                     /translation="MPANLPPQYKEAEERYRQAKTAPEKILALEEMLALIPHHKGTDK
                     LIGQLRKRLSQHKEESQRRSTTSRQADPFTVKKEGAAQVVLVGLPNCGKSQILGCLTN
                     AVPLIADYPFTTRAPLPGMMKFENIQVQIVDSPPLMDEFAETGLANLIRNADAMAIVL
                     DLAEDCATQIELIQEELNRRRVRVLKKGEEKKPEIGQYPKRAVLIGNKADLKEARENC
                     RQLLDRFAKEYPVLCISAKENLNLEEAKKEIFSVLDIVRSYTKAPGKNPDWDDPVILP
                     KGSKVLDFASQIHKDFAQKLKFARIWGKEKYDGQMVPRDYVLKDGDVIELHI"
     gene            complement(4216..4431)
                     /locus_tag="VEL68_08040"
     CDS             complement(4216..4431)
                     /locus_tag="VEL68_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35953.1"
                     /translation="MKFKGKEQCWERMGCGQEHLQGSPAYPDYGRLCWPIAGTKCPAG
                     ARGTHAQKIKDCESCDFFKAVVFDGLD"
     gene            4505..5101
                     /gene="yedF"
                     /locus_tag="VEL68_08045"
     CDS             4505..5101
                     /gene="yedF"
                     /locus_tag="VEL68_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HXZ35954.1"
                     /translation="MAVLDCRGMSCPQPVLETKKALQKSPSGEILVLVDNPASKENVR
                     RFAESQGYRVDISDERGTFQLRLQKAEGPGKDPADEERKVEGETPSVVFIESDSIGRG
                     SEELGRILMRSFLHTLAEGDFKAAKIIMVNSGVKLTCEGSEVLEDLRHLSRQGAEILS
                     CGTCLDYFGLKTKLQVGRVSNMYEILSSLAQAGKVLKM"
     gene            5318..6100
                     /locus_tag="VEL68_08050"
     CDS             5318..6100
                     /locus_tag="VEL68_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011043657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HXZ35955.1"
                     /translation="MPLSPEEREQLTKAKFLLENPSLAAKITGAIGTPIEKGFGRLPA
                     GWRASIQKATQKALMSALNVAVGTMNNKKSNPSSPKLHKGLVALSGAVGGAFGLPALA
                     IELPVSATIMLRSIADIARSHGEEISKIEARIACLQVFALGGSSASDNAVDIGYYSLR
                     ALLARSVSEAAQYIAEKGIAEEGAPAIVRLITKIAARFSIPVSEKAAAQSVPVIGAFG
                     GALINTMFIDHFQDISEGHFTVRQLERKYGVEVVKKAYEQLP"
     gene            6179..>6386
                     /locus_tag="VEL68_08055"
     CDS             6179..>6386
                     /locus_tag="VEL68_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35956.1"
                     /translation="MSGERKEAKTNPRLRAAFLEVVDNQLRDNNPPETKETLARLMAE
                     GYSAESAKDLIAQAVAMESYIVMKT"
BASE COUNT         1547 a         1614 c         1853 g         1372 t
ORIGIN      
        1 acgtcccttc agcaatggcc cagcatcaac atggtgggat gtccggcatg cccgggatgg
       61 aaggggagaa gaaacccgaa tcggcgttag gcaatgaagc atcgcccaac cgcaccttcc
      121 tgttgcaggg gaatgtcaag gcggccttct caatcatggc catggtggag cataagaaga
      181 tgcttcagga aatgaagatg aaaatggaag tcgatccgca ggcgacccac aatatcgcgg
      241 ttacgctcag cgatacccgg acgaacctgc ccatcaccga cgcggtggtc aagatgaaag
      301 tcatcgaccc gaagggaaaa gatcagatca aacccctgga cttcatcgcc ggcatgaatc
      361 agtacgcagg cgacttcacc ctgtccggca agggccggta ccaagtcctg atccttttca
      421 aagcaggcgg caataaggaa tccgcgggct tttattacct ccagaagtga tcgccactct
      481 ttccccctga ttcaaaatgg gcgatagatt gatcgcagcg accctacctg ggtaaattcg
      541 ctgctcctgg cggcaacctt cttgacaatt atgaaaggcc tctggccggg agtcgcctat
      601 catgattgga agaaaatgaa cccttgcaga gaggttgttt attcgcgagt ggatgggaag
      661 atggaaaatt tcccggccat gcgtgccctg gatgaattca agtctcccac ggccatgggt
      721 atctttttcg gcggggactg gtggttaacc gagaaaatct acctgggggg cgacgcccgc
      781 ctgttcgggg agcactccgg aacattctct gcgcagcacc attttgagtg gtataattaa
      841 taaaatatgg aagttgggac gatccatccg tcgagctttg ctcgaattcg tttatcgaag
      901 gagaaccgat tatggagaaa atcaaaatcc aaggaatgac ctgtcagcac tgcgtgatgt
      961 ccgtgaccaa agccctggga aacctcccgg gggtaaagaa cctgagagtg gacctggtga
     1021 agggggaagc cgccttcgaa aatacccaga atatttcccg ggatagcctg cggaaggccg
     1081 tggaggacgc cgggtatacc gtggtggatt aactctctca tggcctgcca ttcccggttt
     1141 ttttgctttc tttgatcttg acatttttga taaggtttaa tataaagact tcatatcgac
     1201 ccggagggaa atatgcgact ttcgacgaaa agtcggtatg gagttcgcgc cctgttcgac
     1261 atcgcctacc attccaaggg gcatccaacg cagatcaaag agatctccag gcgggaggga
     1321 atcacccccc gttatctgga acagattttc cagaaactga aaaaggccgg catggttaaa
     1381 agcgttcgcg ggcccaaagg gggctattat ctctcccgta gaccggaaga aattgtcgtg
     1441 ggggatatca tccgggcgat ggaggagtcc attgagccgg tccactgcgc ccggacgact
     1501 cgggatcgga agaagtgccg ccgggagtcg aggtgcgcgg cccaggtggt ttggaaggaa
     1561 gccggccagc gtctccggga gtacctggat tccacctcat tgggcacgat gtgcaaaatc
     1621 gcgcgaaaca taggggtcgg aaaaccattg gattcccgtt tcatggacgc cgtttaagaa
     1681 aggagggttc ccttttcttg ttgacagcgg tgctcgtgta tagataatat atttatatca
     1741 tcttatccag agtggcagag ggactggccc aaggaagcca cagcaaccgg tcccgcctga
     1801 ggcgggatgc tggtgctaat tccagcaagg taggaatacc ttggaagata aggtgggatg
     1861 aggtctttag caagcccctt cttatcagcc aagagggggc tttttttatt gctgccccgt
     1921 tcttccaggg caaggaaccc aaagcagagg ggtgaaaatg caggggaata aaaataaggg
     1981 aagttaagaa aaggagacca taaaaatgaa tccccacaaa gtcaataaga ctatcgccga
     2041 gatcaacgac aaaatacgcc gaggaaaagc ggtggtagtc actgccgaag aaattatcga
     2101 tatcgtcgaa gaaaaagggc cggccaaggc tgctttagaa gtggatgtgg tgaccacggg
     2161 aaccttcggg cccatgtgtt cctccggagc gttcttgaat ttcggccaca ctcagccgcg
     2221 catcaaggcg gctaaggcct ggttgaacgg cgtcccggcc tatgccgggg tcgccgccgt
     2281 ggatatctac atcggggtca cggagccgac tgaggatgat cccttgaaca aggtataccc
     2341 cggagagttc aaatatggcg gggggcacgt gatccaggat ttggttgcag gcaagccggt
     2401 ccttctccgg gccgaagctt acgggacgga ctgctacccg cggaagaaac tggaaaagga
     2461 gattaccctg aaaaccgtcc aagaagcttt tatcttcaac cctcgcaacg cctaccagaa
     2521 ttacaactgc gcggtcaacc tttcggatcg gacgatttac acctacatgg gagtcttgcg
     2581 gcccaacgcg gggaatgcca actactgcag cgctggccag ctgagtccgc tgatgaatga
     2641 tcccctctac aagacgatcg gcatcggaac ccgggtcttt ctggggggag gacaggggtt
     2701 tatcgtctgg cggggaacgc agcacaatcc caatgtgaag cggggttcga accaggttcc
     2761 ccgcggcggg gccggaaccc tgtcggtatt gggagatatg aagcagatga gtccggaatg
     2821 gctggtggga gcgagcttcc aagggtacgg ctgtactatg atcgtgggca tcggggtccc
     2881 catccccctg ctgaacgaag aaatcgccca gttcaccgcg gtgaaggatg aggatatcta
     2941 cacccagatc atcgactaca gcaaggatta cccggagggg ggcccaacgc agagtttggg
     3001 ggaggtcaac tacaagcagc tcaagagcgg caggatcaaa ttcaacggga aagagattcc
     3061 caccacgcct ctctccagct acccgaaggc ccgcaagatc gccgaggtcc tgaaggggtg
     3121 gattcagaag ggagaattca ccctgggcga gccgcaacag cttttgcctt cggtgagccc
     3181 gacgaggctg taatgtcctg ctctttcaac cttcaaatat gcagttcaat cacatccccg
     3241 tctttgagga cataatcccg gggaaccatc tggccgtcat acttttcctt tccccagatg
     3301 cgggcgaatt ttaatttctg ggcgaagtcc ttgtggattt gggaggcgaa atccaggacc
     3361 ttactcccct tgggcaggat gaccggatcg tcccagtccg gattttttcc cggggctttg
     3421 gtataggatc ggacgatgtc caggacggaa aatatttctt ttttagcctc ctccaggttc
     3481 aagttctcct tggcggagat gcagaggaca gggtattcct tcgcgaaccg atccagcagc
     3541 tgacggcagt tttcccgggc ttccttcagg tccgctttat tcccgatcag gaccgccctc
     3601 ttagggtatt ggccgatctc cggcttcttc tcttctccct ttttcaaaac ccggacccgg
     3661 cggcgattca gctcttcctg gatcaactca atctgcgtgg cgcagtcttc ggccaggtcc
     3721 aaaacgatag ccatggcgtc ggcattgcgg atcaggttgg ccagcccggt ttccgcgaac
     3781 tcatccatca aagggggaga atcgacgatc tggacctgga tgttttcgaa tttcatcatc
     3841 ccgggaaggg gagcccgggt ggtgaaggga taatcggcga tcaggggaac cgcgttggtc
     3901 aggcagccca aaatctgcga cttcccgcaa ttgggcagcc ccaccaggac cacctgggcg
     3961 gccccttcct ttttcacggt gaagggatcg gcctggcggg aggtggtgga gcgtcgctgg
     4021 gattcttcct tgtgctggga gagacgtttt ctcaactggc caatgagctt atccgtcccc
     4081 ttgtgatggg ggatgagggc cagcatctcc tccagggcca ggattttctc gggagcagtc
     4141 ttggcctgcc ggtagcgctc ttccgcttct ttgtactggg gtgggagatt ggccggcatg
     4201 gggaatcggt tcccttcagt ctagtccgtc aaaaacgacg gctttgaaaa aatcacagga
     4261 ctcgcagtcc ttgatctttt gagcgtgggt cccccgtgcc ccggcggggc acttcgttcc
     4321 ggctatgggc cagcacagac gcccgtagtc cgggtaggcg gggctgccct gcaagtgttc
     4381 ttggccgcag cccatccttt cccagcattg ctccttgccc ttgaatttca tgatggggcc
     4441 tcctgaaatt ttgtgataat gttattataa accgacatct aactcaagag ggtagggggg
     4501 cattatggcg gttttagatt gcagaggaat gtcctgtccg cagccggtgt tggaaacgaa
     4561 gaaggctttg cagaaatcac cgtcgggaga aattctggtc ctggtggata atccggcttc
     4621 caaggaaaac gtgcgccgct tcgccgaaag tcagggttac agggtcgata tatcggacga
     4681 aaggggaacc ttccagttac gtctgcaaaa ggccgaaggc cccgggaaag acccggcgga
     4741 cgaggagaga aaggtggaag gggaaacccc atcggtggtc ttcatcgaat cggactcaat
     4801 cggacggggc tccgaggagt tgggaagaat cctgatgcgt tccttcctgc atactctggc
     4861 ggaaggggat ttcaaggcag ccaagatcat catggtcaac agcggagtga aactgacctg
     4921 cgaagggtct gaagtcctgg aggacctgcg ccatctctcc cggcagggtg cggaaatcct
     4981 ctcctgcggg acctgcctgg attacttcgg actgaaaacc aagctccagg tgggcagggt
     5041 ttccaacatg tacgaaatcc tatcctccct ggcccaagcc gggaaggtct tgaagatgta
     5101 aaccgtgccg ggaatcgccg actgtagggg cgaccggccg gtcgccctta gatgggattg
     5161 atggatcgga cttcttacct caaatttggg tcgggctgga agaggataaa cggaatggag
     5221 ccaaaggaaa gcatcgagcg cagaaagagc atctgtgcct tccgccaaga accggtcccc
     5281 tgagtcgcag cagcacccag aagtacagga ggatgggatg cccctttcac cagaagaaag
     5341 agaacaatta acaaaggcga agtttttgct cgaaaatcct agtctcgccg caaagatcac
     5401 gggcgctatc ggaactccaa tcgaaaaagg atttgggcgg ttaccagcgg gatggcgggc
     5461 gtcaattcaa aaagctaccc aaaaagcact tatgagtgct ctaaacgttg cagtgggaac
     5521 gatgaataat aaaaaaagta acccatcttc cccaaagctt cataaaggcc tcgttgcctt
     5581 gtctggcgca gtagggggtg cgtttggttt gcctgcttta gctattgaac tcccggtctc
     5641 ggctacaatt atgctcagat cgatagcgga tattgctcga tcacacggcg aagaaatatc
     5701 caagattgag gcaagaattg cgtgcctcca ggtcttcgct cttgggggtt cgtccgcttc
     5761 tgacaatgcc gtggatattg gttactacag ccttcgggct cttcttgctc gctccgtatc
     5821 ggaagcagca caatacattg ccgagaaagg gattgcggaa gaaggcgccc ctgcaatcgt
     5881 tcgactcata accaagattg ccgcacgatt cagcataccg gtttctgaaa aagcggctgc
     5941 gcaatccgtt ccggtcattg gggcttttgg gggtgcgctt ataaacacaa tgtttatcga
     6001 tcattttcaa gatatcagtg aaggccactt tacggtgcgc caacttgaaa ggaaatacgg
     6061 cgttgaggtt gttaagaagg cttacgaaca gttgccatga aattatttat aacccagcga
     6121 gcagaccatg tgaaggtgga aatatttccc tacgtatccg aatccgggga gagataaaat
     6181 gagcggcgag agaaaagagg cgaagacaaa tccgcgcctg agggcggctt tccttgaggt
     6241 cgtggacaac caactgcggg acaacaatcc gccggaaaca aaggagacgc tggcgcgcct
     6301 gatggcggag gggtattcag cggagagcgc caaagacctg attgcccagg cggtggccat
     6361 ggagtcgtac atagttatga aaacgc
//
LOCUS       DASPRR010000210        11148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1017169, whole genome shotgun sequence.
ACCESSION   DASPRR010000210 DASPRR010000000
VERSION     DASPRR010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017169"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VEL68_08060"
     CDS             complement(<1..901)
                     /locus_tag="VEL68_08060"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HXZ35957.1"
                     /translation="MSDSSKTVRMISLGCPKNLVDSEVMLGLLKQRGWTPSAKDDADV
                     VIVNTCGFIREAKEESIETILTLAEAKEGGRVQKLVVAGCLPQRYGRELLPELPEVDL
                     FVGTGEFHRIADLLDRSARGKLRQKQWIDSPEFLYDHRTPRIHSSPPGSMYLKVSEGC
                     ANFCSYCVIPRIRGRLRSRPISSILKEAEQAASRGVREINLIAQDITAYGKDRRDGSS
                     LVQLLKGLAKVEGVRWIRLLYAHPAHLTKGLVRLIRDEEKVCKYLDLPIQHVDEGILK
                     AMNRPPLKRGMPDLIDWVRREIPG"
     gene            complement(909..1574)
                     /gene="lolA"
                     /locus_tag="VEL68_08065"
     CDS             complement(909..1574)
                     /gene="lolA"
                     /locus_tag="VEL68_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HXZ35958.1"
                     /translation="MISSVLVFSLAQGQSQIPSLNEIVAKVQGQYDAHGDFKADFVQE
                     SLVKSLGRKQVSEGVVFFKKPGKMRWNYQTPFKQEIVSDGKTLWSYHPEEKQVMVTPM
                     SQAAQGKVPSTFLAGLGNLKLDFQVRWAKEPSPQENYFLELTPNEFQGSLEKLFLLID
                     RENFRILQAKIQDIMGNTTQISFSKTQFDNRLSDSLFTFTPPKGVEVFQMPGVSPPGK
                     TGK"
     gene            complement(1803..4019)
                     /locus_tag="VEL68_08070"
     CDS             complement(1803..4019)
                     /locus_tag="VEL68_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXZ35959.1"
                     /translation="MGKAASSDRSNLKREVLGISLLALAVLLALSLFSFNPDDISFDN
                     QPSEPQRTSNLAGVVGSYLADIFFQVFGLTAFLWPFALAFFALRLFRSSEISVSPLKA
                     ICWAGLFLAVSGLLSLGLGKVNLLGASMNSGGALGKILATTGERFLNLTGAVLLLALV
                     LVVCMMVITNLSWVEVSNGIRQLYSSALERWQEFLKERRKRKKEEAREARRKEPVPAV
                     IIEQQVGAPKEKPTVVLQEKQEHFPFLDSRGSYVLPPLSLLEDPEQKEVKVDKESLLA
                     NAKILEKRLSDFGVQGKVNEVRPGPVITMYEYEPAPGIKINKIVGLSDDLALALRAIS
                     VRIVAPIPGKAAVGIEIPNSIREPVYLKDILGVPEFQAFESKLTLALGKDIFGSSFLT
                     SLQKMPHLLVAGATGTGKSVSLNSMICSILYKADPEEVKFLMIDPKRLELSIYEGIPH
                     LLHSVVFDPKAAAMVLRWATQEMEVRYRLMAEKGVRNIERYNQKAEKEKKEHRKKPSL
                     PIPQGGEGTNGQGGEPVQPLPYIVIVIDELADLMMVSARDVEASLTRLAQMARAAGIH
                     LLLATQRPSVDVLTGVIKANFPTRISFQVSSKTDSRTILDANGAEHLLGSGDMLFLPP
                     GTSRLVRVHGAYVSEVEILRVVEHWKKQGRPVYDPSILEAKEEAQETDADGYDEMYDQ
                     AVALVTETRQASISMIQRRLRVGYNRAARMIEKMEEEGVVGPADGSKPREVYMKKF"
     gene            complement(4430..5803)
                     /locus_tag="VEL68_08075"
     CDS             complement(4430..5803)
                     /locus_tag="VEL68_08075"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXZ35960.1"
                     /translation="MGAWENYLTQNRQRFLDELMEFLRIPSISALPAHAAEVRRAAQW
                     VEKRMKDAGIESVRIMPTGGHPVVYGEWLHAHGKPTILIYGHFDTQPVDPLNLWDSPP
                     FEPVIKDGRIYARGASDNKGNLFIPLTVAEAMLKTAAKLPLNLKFFFEGQEEIGSPQL
                     QPFIAAHKDLFSCDMVFSADGGQWSEDHPGLVLGTRGLASVFVDVQGPDHDLHSGTYG
                     GAVANPIHALVRILDSLHDRDGRITVEGFYNKVRSLSSEERAQMARVPYEEAEYLGEP
                     GSVSVFGEPGFSTYERTWARPTLEINGIWGGFQGEGLKTVLPSMAHAKISCRLVADQD
                     PSQIADLVANHIKKVAPPGVKVSFGNRGSGSFPYLIPPDHRGLGIAASVLKDLYGKEP
                     YIIRSGGTIPVNPVFLEHLGAYTVIFGFGLEDERLHSPNEFFRLSSFEKGQKAYGMLW
                     ERLGGGA"
     gene            complement(5895..6725)
                     /locus_tag="VEL68_08080"
     CDS             complement(5895..6725)
                     /locus_tag="VEL68_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXZ35961.1"
                     /translation="MSQKSSFELLAFLRALILILSAGVFTSILGPPALVSCFLDRSWR
                     WLSFFQRLWVEWLLKTNGIRLRLDGMGNLKKDEAYILVSNHASILDIPALISAAPFPV
                     RFLAKKSLLWFPIFGWVLYFSGHILIHRQSAQSALRSLKKAPSLLKKGISIIVFPEGT
                     RSPDGEVKEFKRGAFLLAQYSKFPVVPISISGTYDMLPRHGWCFWPGTIHIRMSKPIP
                     TGGLSHQELDDLMRGVRETIIENLKKGTAERDLSEEERGKRPDDGMEDAGGRVEQPQG
                     "
     gene            complement(6900..8411)
                     /locus_tag="VEL68_08085"
     CDS             complement(6900..8411)
                     /locus_tag="VEL68_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35962.1"
                     /translation="MKKGFLLLLTLMFVTLAFGPANAARDMDTIVIVQGVDPTTLDPH
                     NHMETPAWNVHLQMFDTLLQRDSEIKIKPMLAESYKVVNDLTWEFKLRKGVKFHNGED
                     FNAAAVKFVLERMADPKLKLRQTVFQGVIDRVDVVDDYTARIVTKKPYPVMDAMLCIY
                     GQMIPPKYFQEKGPAHFALNPIGTGPYKFVRWVKDDHILLEANETYWRGAPKIKKVMF
                     RPIPEATTRVAALQTQEADIVVNIPPHLMKLMDWKGRSFVSKAPSVRVIFMAFDTIKP
                     GPQADKRVRQAIAMGVDMRTNIKKVLEGNGVLLGSPLTDKHYGYDPTIKPYEYNPEKA
                     KKLLAEAGYPNGFDFTLNSPTGRYLNDKEMAEAVAGDLRKIGINATVKTHEWGTYMNR
                     MYSHNNAPAYILGWGNTSFDADFTINPLMRTGLLLCNVSFPKLDALIDEGISTMDPKK
                     RQKIYSDAAKVIKEEVPWAWTYQQIDIYGVNERLNFKARTDELMPVFDMSFKK"
     gene            complement(8751..9668)
                     /locus_tag="VEL68_08090"
     CDS             complement(8751..9668)
                     /locus_tag="VEL68_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35963.1"
                     /translation="MDVVEGTAKALDFVTEDRRAMRWRKVLRKLVENKGAVFGLIMVL
                     GVIFSAVLAPWISPHDPILQDVEKRLFPPMWQEGADPNYLLGTDHLGRDIVSRLIYGA
                     RISIVVSVSAVALSAVLGTLIGLLSGYYGGKVDNIFMRIADVQLAFPFILLAIAIIAV
                     LGPNLQNIILTMAITGWVIYARVVRAEVLSLREKEFITSVRALGGSNGRIIFKHLFPN
                     VVPPIIVIVTLEMARMIIMEAALSFLGLGIQPPTPTWGGMLADGRVYLVTSWWLATFP
                     GLVIMLVVLGINLLGNWLRDMLDPRLTQL"
     gene            complement(9817..10767)
                     /gene="nikB"
                     /locus_tag="VEL68_08095"
     CDS             complement(9817..10767)
                     /gene="nikB"
                     /locus_tag="VEL68_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HXZ35964.1"
                     /translation="MLALFGAVFEGMKGYILKRFFHSIFVLVGISLVVFIILHLTGDP
                     AALLMPMDATPEQVAQFRKEMGFTDPIIVQYWRFFKGTLRGDFGQSFRHSQPALDLVM
                     ERMPATIQLTAAAMVIALVVAIPVGIISAIRRNSILDHIGMTGALLGQSTPVFWLGIM
                     LILIFSVTIQWFPSSGRGEIQHLVLPAITLGMFTMARTARMMRSSMLEVMGQEYMKTA
                     KAKGLSPGMVILKHALKNAAIPVVTIVGMELGTLLGGAVITETIFAWPGVGRLAVQAI
                     YNRDYPVVQAAVFLLASIFVLVNLIVDLMYTYLDPRVKLG"
BASE COUNT         2471 a         3136 c         3054 g         2487 t
ORIGIN      
        1 ctccggggat ttcccttcga acccagtcga tgaggtccgg cattcctctt ttcagcgggg
       61 gacggttcat ggccttcaga atcccttcgt ccacatgctg gataggcaaa tccagatact
      121 tgcagacctt ttcctcatcg cggattaagc ggaccagccc tttggtaaga tgggcgggat
      181 gggcatacaa gagacggatc catcgaactc cttcgacctt ggccagccct ttcagcagct
      241 gaacgaggct ggacccatcc cttcggtcct ttccgtaagc ggtgatatcc tgggcgatga
      301 ggttgatctc cctcaccccc cgggaggcgg cctgttccgc ctccttgagg atggaggaga
      361 tcggccggct gcgaagtctt ccccgaatgc gggggattac gcaataggaa cagaagttcg
      421 cgcatccctc ggaaaccttc aggtacatgc tgccgggagg agaagaatgg atgcggggag
      481 ttcgatgatc gtaaaggaat tcaggagagt cgatccattg cttctggcgg agcttccctc
      541 gggcggaccg atccagaagg tccgcgatcc gatgaaactc ccccgttccc acgaagaggt
      601 ccacttccgg aagttccggg agcaattccc gcccataccg ctgggggaga cagccggcaa
      661 ccacgagctt ctggactctc ccgccttcct ttgcctcggc cagagtgagg atggtctcga
      721 tggactcctc tttggcctcc cggatgaagc cgcaggtatt gacgatgacc acgtcggcat
      781 catccttggc cgagggtgtc cacccccttt gcttcaggag cccgagcatt acctcgctgt
      841 ccaccaggtt cttgggacac cccaggctga tcatccggac ggtcttggat gaatcgctca
      901 tgaaaggatt actttccggt cttccccggc ggggaaactc ccggcatttg gaagacttcc
      961 actcctttgg ggggagtgaa ggtgaagagc gaatcggaga ggcggttgtc gaattgggtc
     1021 ttggaaaaac tgatctgggt cgtgtttccc atgatatcct gaattttagc ctggagaatt
     1081 cgaaaattct cccgatcgat gaggaggaac agtttctcca ggcttccctg aaattcgttg
     1141 ggagtgagct ccaagaaata gttttcctgg ggagaaggct ccttcgccca tcggacctga
     1201 aagtccagct tgaggtttcc caatccggcc agaaaggtgg aaggcacctt tccctgggcg
     1261 gcctgggaca tgggggtcac catcacctgc ttctcctcgg gatggtaact ccagagggtt
     1321 ttcccatcgg acacgatctc ctgtttgaaa ggcgtctggt agttccagcg cattttcccg
     1381 ggttttttga agaagacaac tccttcggac acctgtttcc ttcccaggct cttcaccagg
     1441 gattcctgaa cgaaatcggc tttgaaatcc ccgtgggcat catattgtcc ttgcaccttg
     1501 gccacgatct cattgaggga aggaatttgg ctttgaccct gggccaggga aaaaaccagg
     1561 accgatgaaa tcaaaaccac ccctaaactc gttaaccttg tcatggtttc agtttttcca
     1621 ttgaaagctg gcttcgcctt ccgtaccgcc cggaaatctt tttccatcca tccttttctc
     1681 tttgcggtct ctcacaacgg tcaacctaaa ggatgcaaaa aatatccgcc ggagatcacc
     1741 ccctccctta tttcacccat caaaagggga ggggatcgaa tggaaatttc aaatatcttc
     1801 ggctagaatt tcttcatgta aacttcccgc ggcttgctgc cgtcggccgg gccgaccacg
     1861 ccttcctcct ccatcttttc gatcatccga gccgcccgat tgtaacccac cctcaaacgc
     1921 ctctggatca tggagatcga ggcctgacgc gtttccgtga ctagagcgac cgcctggtcg
     1981 tacatttcat cgtatccgtc ggcatcggtt tcctgggcct cttccttggc ctccaggatg
     2041 gacgggtcgt agaccggccg gccctgtttc ttccagtgct ccaccactct caggatttcc
     2101 acttcggaca cataggctcc atgaacccgg accaggcggg aagtccccgg aggcaaaaag
     2161 agcatgtccc ccgatcccag gagatgctcg gctccgttgg catccaggat cgttcgggag
     2221 tcggttttgg aggaaacctg gaaggaaatc cgggtgggga agttggcctt gatcaccccg
     2281 gtgagaacat ccacggaagg ccgctgagtg gccagcagca ggtggatgcc cgcggcccgg
     2341 gccatctggg ccaggcgggt gagagacgct tccacatccc gggctgagac catcatgagg
     2401 tcggcgagct cgtcgatgac gatgacgata tagggaagag gctggaccgg ctctcctccc
     2461 tggccgttcg tgccttcccc cccctggggg atgggcaggg agggcttctt ccggtgttcc
     2521 ttcttttcct tctccgcctt ctggttgtag cgctcgatgt tccgaacccc tttttcagcc
     2581 atcaggcggt aacggacctc catctcctgg gtggcccagc ggaggaccat ggccgctgct
     2641 ttgggatcga aaacgaccga gtggagaagg tggggaatcc cctcatagat cgacagctcc
     2701 aggcgtttgg ggtcgatcat caggaatttc acctcttccg ggtcggcctt gtagaggatg
     2761 ctgcagatca tggagttcag ggagacgctc ttcccggtgc ccgtggcccc ggcgaccagg
     2821 aggtggggca tcttttgcag gctggtgaga aaggaactcc cgaagatatc cttgcccaag
     2881 gccaaggtca gcttggactc gaaggcctgg aattcgggga cacccagaat gtctttgaga
     2941 tagaccggtt cgcggatgga gttgggaatt tcgatcccca ccgccgcctt ccccgggatc
     3001 ggggcaacaa ttcgaacgct gatggcccgc agagccaaag ccaagtcgtc gctcagaccc
     3061 acgatcttgt tgattttgat cccgggcgcc ggctcgtact cgtacatggt gatcaccgga
     3121 ccggggcgga cttcgttcac cttgccttga accccaaaat cggagagccg tttttccaga
     3181 atcttggcgt tggccaagag actctcctta tctactttaa cctccttctg ctccgggtct
     3241 tcaaggaggg agaggggggg cagtacataa gaaccccggg agtccaaaaa ggggaaatgc
     3301 tcctgcttct cctgcagaac gacggttggt ttttctttcg gtgcacccac ctgctgctct
     3361 atgatgaccg caggaacagg ctcttttctc ctggcttctc tcgcttcttc cttctttctc
     3421 tttcttcgct cctttaagaa ctcctgccac ctctccagag cggacgaata gagttggcgg
     3481 attccgttac tcacctccac ccaggaaagg ttggtgatca ccatcatgca cacgaccaat
     3541 accagagcca ggaggaggac cgccccggta aggttgagaa accgttcccc cgtggtagca
     3601 agaatctttc ccagggctcc gccgctattc atggaagccc ccaagagatt tacctttccc
     3661 aggccgaggg aaagcaatcc gctgaccgcc agaaacaatc ccgcccagca gatggctttt
     3721 aagggagata cggaaatttc ggatgaacgg aagaggcgca gggcaaaaaa agccagggca
     3781 aagggccaga gaaaagcggt caatccgaag acctggaaaa aaatatcagc caggtaagac
     3841 ccgactaccc cggccaggtt ggaggtcctc tggggttcag acggctggtt gtcgaaggaa
     3901 atgtcgtccg ggttgaagga aaaaaggctc agagccagga ggaccgcaag ggccaggagg
     3961 gagatgccga gaacctccct tttcaagttg gaccgatctg aggatgctgc ctttcccatt
     4021 tgaaaaaatg ataatccgga aaaatgggaa agtcaaaaga aaacggaaag atagtgtcat
     4081 aagccaggca aatagtcgac ggtgcagcgg tatccgacta taagactatg tgactatgcg
     4141 actatactga tgggcccata attgagaaga catattcggc caaaaatccc tctaatcccg
     4201 atgctcttca ctttacctgg aaccgttcac cctgagccct tcgattcacc gttcgtcctg
     4261 agcttgtcga agggcgaacg gctcactcag gacatgcttg tcgaagggtg cgtcgctacg
     4321 ccgttcatgc ttcgataagc gttcgactga gctcacgccg aaggctcagc acgaacggct
     4381 tattcttcag cgtccatata attatggacc gatgggcaag actaatcgcc tacgctcctc
     4441 ctcccagcct ctcccagagc atcccgtagg ccttttgtcc cttctcgaaa ctggagagtc
     4501 ggaagaattc attgggcgag tgcaggcgtt catcctccag gccaaaacca aagatgaccg
     4561 tgtaggcgcc cagatgttcg aggaaaacgg gattcaccgg aatcgtcccg cccgagcgga
     4621 ttatgtaggg ttccttgcca tagaggtcct tgaggaccga cgcggcaatt cccaagcccc
     4681 ggtgatcggg ggggatgagg taggggaagg acccgctgcc gcgatttccg aaggaaacct
     4741 tcacccccgg cggagcgact tttttgatgt ggttggcgac caggtcggca atttgggaag
     4801 ggtcctggtc ggccacaagg cggcaggaaa tcttggcgtg agccatactg ggcaggacgg
     4861 ttttcaatcc ttcgccctga aaaccgcccc atatgccgtt gatctccagg gtcggccggg
     4921 cccaggtccg ctcgtaagtc gaaaaccccg gttccccaaa gaccgaaacc gatccgggct
     4981 cgcccaggta ttcagcctct tcgtagggaa cccgggccat ctgggcccgt tcttcgctgc
     5041 tgaggctccg caccttgttg tagaatccct ctaccgtgat tcgtccatcg cggtcgtgca
     5101 gggagtccag gatccggacc agggcgtgga tagggttcgc cactgccccc ccgtaagttc
     5161 cggaatggag gtcgtgatcc ggcccctgga catcgacgaa aaccgaggcg agcccccggg
     5221 tgcccaggac gagacccggg tggtcctcgc tccactggcc cccgtcggcg ctgaacacca
     5281 tgtcgcagga gaaaaggtcc ttatgcgccg ctatgaaggg ttgcagttgg gggctcccga
     5341 tctcctcctg gccttcgaag aagaatttca ggtttagggg taatttcgcg gcggttttga
     5401 gcatggcctc ggcgacagtc agggggatga agaggtttcc cttgttgtcc gaagcgccgc
     5461 gggcgtagat tcggccgtcc ttgatcaccg gctcgaaggg aggactgtcc caaaggttca
     5521 gggggtctac aggctgggtg tcaaaatgcc cgtatatcag gattgtaggt ttgccgtgcg
     5581 catgaagcca ctccccatag accaccgggt ggccgcccgt gggcatgatc cggacggact
     5641 cgatccccgc atctttcatg cgcttttcca cccactgggc cgcccgccgc acctcggccg
     5701 cgtgggcagg caaagcggat atgctgggaa tccgcaggaa ttccatcagt tcgtcgagaa
     5761 acctctgcct attctgagtc aggtagtttt cccatgcgcc cattgcttga atctcctatc
     5821 ttttctttat ttcccttctc cgcgtctttc ttccctcgcg tcttccttct tccctagtta
     5881 ctattccctg agcgttaccc ctggggctgc tccaccctcc cccctgcgtc ttccatccca
     5941 tcatccggcc tttttcccct ctcctcttcg ctcaaatctc tttctgcggt tccctttttg
     6001 agattttcga taatcgtctc ccggaccccc ctcatcaaat catccaattc ctgatgagac
     6061 aggcccccgg tggggatggg tttgctcatg cggatgtgta tggttccggg ccagaagcac
     6121 cacccgtgcc ggggaagcat atcataagtt ccggaaatcg aaatcgggac cacgggaaat
     6181 ttggagtatt gggccaggag aaaggccccg cgcttgaatt ccttcacctc cccgtccggg
     6241 cttcgagtcc cttccggaaa aacaataatt gaaatcccct ttttgaggag ggaaggagcc
     6301 ttcttcaggc tcctgagcgc tgactgagca ctctgccggt ggataagaat atggcccgag
     6361 aagtacagga cccagccgaa gatggggaac cagaggaggg attttttggc caggaaacgg
     6421 accgggaaag gagcagcgga aatgagcgcg gggatatcca ggatactggc gtggttggaa
     6481 accaaaatat aagcctcatc tttcttcagg ttccccattc catccaggcg caagcgaatg
     6541 ccgttggtct tgagaagcca ttcgacccag agcctttgga aaaaggaaag ccaccgccag
     6601 gatcggtcca ggaaacagct gaccaaagcc ggggggccga gaatagaggt gaaaacgccg
     6661 gcggaaagga taaggattaa ggcccggagg aaagcaagca gctcaaaaga acttttctga
     6721 ctcataggta ggttaagaga acctgcgggg gcggttcgga cttcggcgag ctcagccgag
     6781 ccgcgaaccg cccctccacg ccaggaggat gtttaggcga caaattgcag gagcgaccac
     6841 gtggggctgc ccccacaata gggtttttgg ttccggctat gtcaaattcc ggttaaggat
     6901 tatttcttga agctcatgtc aaacaccggc atgagctcat cagtacgggc tttaaagttt
     6961 aaccgctcat tgactccata gatgtcaatc tgctggtagg tccaggccca gggaacttct
     7021 tctttgatca ccttcgccgc atcggaatag atcttctgcc gctttttcgg gtccatggtg
     7081 ctgatgccct cgtcgatcaa agcgtcgagt ttgggaaagg agacgttgca taacagcaag
     7141 ccggtccgca taaggggatt gatggtgaag tcggcgtcga agctggtatt cccccaaccc
     7201 aggatatagg ccggagcgtt gttatggctg tacatgcgat tcatataagt gccccactca
     7261 tgggtcttca ccgtggcatt gatgccgatc ttccgcaagt ctccggctac cgcctcggcc
     7321 atttccttgt cgttcaagta gcgcccggta ggggagttaa gcgtgaaatc aaagccgttg
     7381 ggatagccgg cttcggccag aagcttcttg gctttctctg gattatactc ataaggtttt
     7441 atggtggggt cgtacccata atgcttgtcc gtgagggggg aacccaacag tacgccgttg
     7501 ccctccaaga cctttttgat attggttctc atgtccacgc ccatggcgat ggcctggcgg
     7561 actctcttat ccgcttgggg gcctggcttg atggtatcaa aggccatgaa gatcacccgc
     7621 acgctggggg ccttggagac gaaggagcgt cccttccagt ccatcaattt catcaagtgg
     7681 ggaggaatat tcacaacgat atccgcctcc tgggtctgca gcgcagctac ccgggtagtc
     7741 gcctcaggaa tggggcggaa catgaccttt ttgattttgg gggctccgcg ccagtaggtt
     7801 tcgtttgctt ccaaaagaat atggtcgtcc ttcacccagc ggacgaattt gtagggaccc
     7861 gttccgatgg gattcagggc gaaatgagcc gggcccttct cctggaagta tttgggggga
     7921 atcatctggc cgtaaatgca taacatggca tccatgaccg gatagggctt tttggttacg
     7981 atccgcgcgg tgtaatcatc caccacatct acccggtcga tgaccccctg gaagacagtt
     8041 tgccggagtt tgagtttggg gtcggccatg cgctccagga caaatttgac cgctgccgcg
     8101 ttgaaatctt ccccgttatg aaatttgact cccttccgca gcttgaactc ccaggtaagg
     8161 tcattcacga ccttgtacga ctccgccagc ataggcttaa tcttgatttc ggagtctctc
     8221 tgaagcaggg tgtcaaacat ctgcaggtgg acattccagg ccggagtctc catgtggttg
     8281 tgggggtcca gggtcgtggg gtcgacgcct tgaacgatca cgatggtgtc catgtcccgg
     8341 gcggcgttcg ccgggccaaa ggccagcgtg acgaacatga gggtaagcaa caacaaaaat
     8401 ccttttttca taaacccctc ctccttctct tttattgtct ttctaaaaaa ttttttcaac
     8461 ttgaaactta gtgaactcgt aaaaagtcaa aattcgatag aattcgtcat tccggcgaaa
     8521 gccggaatcc acttatttca aaatgttctg gaccccggct ttcgccgggg agacgcttca
     8581 aagggctttt ttacgagatc atcaaatttg caacccgcaa ccctttttct cttaaccccc
     8641 caccccaacc ctccccctct gaggggggag ggaagggtgg gggtgatgct tttataattt
     8701 ttttgcagcg tttgggtaaa atctgcgttc atctgcgtcc catttaactc ttacagctgc
     8761 gtcagccgcg ggtccagcat gtccctcagc cagttcccca agagattaat acccagaaca
     8821 actagcatga tcaccaatcc cggaaaagtg gccaaccacc aggaggtgac caggtagacc
     8881 cgcccgtcgg ccagcatgcc tccccaggtt ggggtggggg gctggatccc caggcccagg
     8941 aagctgagcg ccgcctccat gatgatcatc cgggccattt ccagggttac gattacgatg
     9001 atcggcggaa ccacattggg aaaaagatgt ttgaagatga tccggccgtt ggagccgccc
     9061 agggccctca ccgaggtgat gaactctttt tcccggaggg acaaaacctc cgcgcggacg
     9121 acccgggcat agatcaccca tccggtaatg gccatggtta ggatgatgtt ctgcaggttg
     9181 gggcccagca cggcaatgat ggcaatggcc agaaggataa agggaaaggc cagctgcaca
     9241 tcggcgatgc gcatgaaaat attgtccacc ttccccccgt aatagccgga taagaggccg
     9301 atgagagtcc ccaggaccgc tgacagggca accgcggaaa cgctgaccac gatggagatc
     9361 cgagccccat agattagacg actgacgata tctcgcccca agtggtccgt tcccaaaagg
     9421 taattggggt ctgctccctc ttgccacatg ggggggaaaa gccttttttc cacatcctgc
     9481 aggatcgggt catgggggga aatccatggg gcaagaacag cggaaaagat aacccccagg
     9541 accatgatca gcccgaacac ggctcccttg ttctcaacca acttgcgcag cactttccgc
     9601 cagcgcatgg cccgtcgatc ctcggtcaca aaatccagcg ctttggcggt gccttctaca
     9661 acatccatgc ctttgtccta ttcatccaac ttttcgctcg caactggaga aaattcgaaa
     9721 tccgaatatc gaatttcgaa acaaatccga attttcaaat ttcaaaacgg aggtttcgga
     9781 tttcgggttt cgtgcttcga atttcataat cttttttcac cccagcttca ctcttgggtc
     9841 caaataagta tacataaggt caacgataag attgaccagc acaaagatcg atgccaggag
     9901 gaatacggcg gcctgaacca ccggataatc tcggttgtag atcgcctgaa ccgccaggcg
     9961 ccccactccc ggccaggcaa agatggtctc ggtgatgacc gctcccccca ggagagtccc
    10021 cagctccatg ccgacgatgg tcaccacggg aatcgccgca ttcttcaggg cgtgcttcaa
    10081 aatgaccatc cccgggctta accctttggc tttagccgtc ttcatgtatt cctgccccat
    10141 cacttcaagc atgctggacc gcatcatccg ggcggtccgg gccatggtga acattcccag
    10201 ggtgatggcc ggaaggacca ggtgctgaat ctctccccgg cctgaagagg gaaaccactg
    10261 gatcgtcacg gaaaagatca ggatcagcat aatccccagc cagaagacgg gggtggattg
    10321 gcccaacagg gccccggtca ttcctatgtg atccaggatg gaattgcggc ggatcgccga
    10381 aataatcccc acgggaatgg ccacgacaag ggcgataacc atggcggcgg cagtcagctg
    10441 gatggtggcc ggcattcgct ccatgaccaa atccagagcg ggctggctgt ggcggaaaga
    10501 ttggccgaaa tctcccctga gggttccttt gaagaagcgc cagtactgca cgataatcgg
    10561 atcggtgaag cccatctcct tgcggaactg ggccacctgt tcgggggttg cgtccatagg
    10621 catgagcagg gctgcggggt ctcccgtgag gtggagaatg atgaagacaa ccagggagat
    10681 tcccaccagc acaaaaatgg aatggaagaa ccgctttaaa atgtatcctt tcatcccctc
    10741 aaataccgcc ccgaacagag ccagcacatg ggcttaactc tttcaaagta ctctacaaat
    10801 cgggcaaaaa aattatataa cataaatgga gcgggttaag caatgagaaa catcccttgc
    10861 cctccttctc ctttcgacta atataaacgc aataaataaa atgacatcac cccctcccta
    10921 accgttcggc tgagctcacg acgaagccct cctcccttgg aggttgtgtc ataatccgtt
    10981 catggttcga caagctcacc atgaacggat tatgagcgca ttaaaattca tcaacttagc
    11041 cgttcgcccg ctcggccgag ctcgcgacga agcctgagcc tgtcgaaggg cgaccggggg
    11101 attacgacac agtctcttgc gggagagggg agggtggggg gtaaaata
//
LOCUS       DASPRR010000211        15340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1017418, whole genome shotgun sequence.
ACCESSION   DASPRR010000211 DASPRR010000000
VERSION     DASPRR010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15340)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15340
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017418"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VEL68_08100"
     CDS             complement(<1..791)
                     /locus_tag="VEL68_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXZ35965.1"
                     /translation="MNIAEILPRTAKRLPDKIAFVFNDQPTRYKALQDLTEAFAHGLQ
                     KLGVEKGDRVAVFAPSSLEFVVSWFSVVKLGAVFVPVNIMLKARESKYILENAEVNTL
                     IVHQSQGEIIQSIRPGLKNMIVIGNQTLPDSLLFSALLAEKPVREMTVDCAPDDTATM
                     IYTSGTTGFPKGAMITHGNFDSNVKGIIVALNLDEEMVRVSVTPLFHAMGLTVNMLAV
                     VMLGATAVIQAKLDFEEFLKANEKYSATMISGAPTLHYMLVNDPRT"
     gene            complement(852..2507)
                     /locus_tag="VEL68_08105"
     CDS             complement(852..2507)
                     /locus_tag="VEL68_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXZ35966.1"
                     /translation="MDELKKIAEEKTRWAKEKKIGELPSARSEMGRPLPPVFAPDDLQ
                     GIDYLRDIGFPGEYPYTRGVYPGMYRKGPWQMRLYSGFGTAEDTNRRWKFLLKSGNMG
                     VACAFDLPTQIGLDSDDPLAQGEVGKVGVAIDTLRDMEILYDGLPLDQITSSFNINTP
                     AAALLAMYLVLAEKQNVPLAELNGTLANDMLCEYISRGTWVFPPGPALRMSTDIVEFC
                     SRHLPRFYPYNIRGIILREAGASMIQEGAYAFANAVAYIEDALKRGLILDDFGPRISF
                     FFATGLQVFEEAARYRALRRLWARMMKERFGARNPNSMLLRFTGTVGGSTYRAREPEN
                     NLVRGAYGLLANILGGAQGMLQPAMDEAYAIPTEHTARLALRTQQILAYETGVTKIAD
                     PLGGSYFVEALTQELEGTIKGEMEKVESIGGAVKAIEMGYPQMAIAEQAYRVAREEER
                     GERIVVGVNKFQAGEEGKRQLIIHRADPTSVERQIQRTRAVKAARDNLKAQKALDELC
                     RIAETKENLMPCFIQAVKAYASVGEITEALKKVFGEFKEPVSI"
     gene            complement(2681..3958)
                     /locus_tag="VEL68_08110"
     CDS             complement(2681..3958)
                     /locus_tag="VEL68_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ35967.1"
                     /translation="MKRLSKWVSAEEALEAVNSGERLILPLCCGLPQTLVEALIARKD
                     RLRGVEIVSGLQIEYPFLQEGTEESFSFRTWQCAPRIRHLLKKGTVKYIPMRQGDAPF
                     VFSRKGIWPIDGALVQLSPPDKNGYLSFGVSIGHSLPTALEAKLVIAEVNDQMPRVLG
                     DAFIHLSQVDYLVETSRPLLEYPSTEKVGEVERKIGNYVAELIPDGTTLQIGIGSIPD
                     AVVQSMEGKRDLRFFAMGIDAMVDCVEKGLVERSISPDQQPKMIVTEILGSKKLFDFV
                     HENPMVEGRPIMTTINSRVVGQIEKFISLLGAIEVDLTGQVNAETVGGRQISAIGGSF
                     DFLQGALFSPQGKSIIAVTSATPDGKYSRIVAQLPPGSAVTHPRHSIQYVVTEYGVAD
                     LRGKTLRERAEALTAIAHPNFRESLEKFAHENY"
     gene            complement(3955..4380)
                     /locus_tag="VEL68_08115"
     CDS             complement(3955..4380)
                     /locus_tag="VEL68_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXZ35968.1"
                     /translation="MLRETKAGDPLPVIEKEIDQDRIMLWAKISGDFNRLHVDPKYAG
                     QTRFGGTIAHGPLSLAFFNELMMECFGMGWAEGGKLLDVRFVAPIRPGDKIRISGTVM
                     QVQMTGRKWSVECDLAVEKQNGEKAVTGRAIGFLGAEGK"
     gene            complement(4464..4880)
                     /locus_tag="VEL68_08120"
     CDS             complement(4464..4880)
                     /locus_tag="VEL68_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXZ35969.1"
                     /translation="MEIQEDKPELLFADLPVGRIFRPLEYPITPELVQDFIETVGDRH
                     QLYDEGFAPPGLAAIYARLSYLQDHTMPSGGVLAKQGFEFQNPIKIRDILKVQAKVIE
                     SYQDEKMRKRVTFLIEAQDQKGMPVSTIRLYAIWPK"
     gene            complement(5013..6302)
                     /locus_tag="VEL68_08125"
     CDS             complement(5013..6302)
                     /locus_tag="VEL68_08125"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXZ35970.1"
                     /translation="MKGMPERVNICECWARDGLQGEGQFIPTEKKIAIINRMVEIGFK
                     RIEATSFAHPKLIPQFSDSLEVLKGIRRPPDVTYVAIIPNDKALDRLLDICHQGYGVQ
                     EITAILSASEDHLLANLERTFEEAMPPLADLVKRARQAGIKVIGCIGTAFGCPLAGEV
                     PLEKIIELTGWYLDKGATSIMLGDTTGEANPRQVREIFGHMKDRFPGVDFVAHFHDTR
                     GMGLANTLAALQEGIVLHDSSFGGMGGQPATRRPKYHKGFAGNTCTEDMVLMMEEMGV
                     QTGLNIDDVIDTGLMAEEFCGRNLLGHVTRSGPVRHCSSRPLSLQDLRPNGEIAPALL
                     FAGKMKEGLSGQALVGYVIREALAKNWSVPENLRIFTDGISVKGEFEGRDILVTRFKV
                     LEIQKGKNEGTLEVLCQRANGDVFMEGQARLTFSGSA"
     gene            complement(6431..7105)
                     /locus_tag="VEL68_08130"
     CDS             complement(6431..7105)
                     /locus_tag="VEL68_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXZ35971.1"
                     /translation="MSAPNRAQKNRPLRETIYADLRRQVLSGRLLPGSRLVESSLAES
                     LKVSRTVIREVIKQLELEGLVKITPYKGTEVSRFSLQDIEELYVIQGALEGLAGGLAA
                     QRMKKEDLREVRFIHERLKKSVRKDPGEWQRLNIAFHQFFIDKCENRRLHGLIKNHRD
                     QFARYWRIILSIPGQREKSNEDHEQILKALDRGEPLKVRLAMEEHIGQSARNLTTFLE
                     KHEFLI"
     gene            complement(7354..8916)
                     /locus_tag="VEL68_08135"
     CDS             complement(7354..8916)
                     /locus_tag="VEL68_08135"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXZ35972.1"
                     /translation="MEYRPEEVTTVSLLELAARKYPDREFLICDEVRRTFKEVDDRVN
                     SLANALVEMGIQRGDRVGSFLANCPEILENYMASNKAGALPVPINFRLSPREIAWILN
                     HAKAKALFFGEEFLPVVEKLRSEMPTIQNYIVVGKHAPGDMHLYEEVVARHSSRHPGL
                     PIGPDDPAMLMYTAGTTGFPKGVFCRHKSNMWSLVNSILYDTMLGRFNIQECMAYPLP
                     FFHKAAYITAVSQLIKFGRCVILRAFDAAKLMETIQKEKVNQFAMVPIVAAAILQVPN
                     LEKYDLSSWHTVRCTGAPLPVALKEKFAKTFPRVDICDNYGMTEIATIAMHVTKGKVL
                     NATTVGKPHPLTEVKILDESRRELPAGEIGEICVRSPALFSEYFENPEANAEAFAGGW
                     FHTADMGRFDGEGYLYIKDRKKDMVISGGENIYPAEVEQVLFAHPKIQEASCIGIPDP
                     QFGEAVMAVVVPKTGQSLSEAEVIEYASQHLAKFKVPKRVAFVGGLPRSAVGKVLRRE
                     LRKQFGGMTVKY"
     gene            complement(9266..10837)
                     /locus_tag="VEL68_08140"
     CDS             complement(9266..10837)
                     /locus_tag="VEL68_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ35973.1"
                     /translation="MDNLKTLLTEMVGLRGNKEYLFFRDRQLTYRELDLLSNRFSRVF
                     RELGVRKGDHVAVMLPNCPEWIACWFGLAKLGAVLVAFNTQWKADGLEYALRQADVKY
                     CLVGAEFQDEFEKAGKAPGLTLVVFDLAEGGVEIEGAHSLSRLLAGISSESVEGGPPK
                     GGDPLIITYTSGTTGWPKAVLNPHRAYIAAAEDLRDYVEMKPEDIIYTCLPLYHANPQ
                     VYCVLTALVTGGSIALAERFSASRFWQEIRAYRATAFSYVGAVLPILLNQPEREEEKG
                     SPARKCFGGGAPKEVHGTATRRFGLQVCELYGMSETGTWNTINRPGKIKSGSVGQVRE
                     CFEVKVFDDEDDEVPPGQVGEIVVRPKKPFVMFNGYYKMPEETLRDYRNLWFHTGDLG
                     KVDSEGYFYFCGRKKESIRRGGENITPYEIEKVLTDHPAVAEAAAVGVPDPILGEEIK
                     VYVVLHEGRSVEPRELIERCQEKLPKFQIPRYLEFVSSLPKTASEKVQKASLKSRGIG
                     SAWDRLGREGSIKSR"
     gene            complement(10840..11955)
                     /locus_tag="VEL68_08145"
     CDS             complement(10840..11955)
                     /locus_tag="VEL68_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35974.1"
                     /translation="MDFEMSEAQKAFQASVRAFSETVIEPEAREYDEKGLFPRETFRK
                     MGREGYLGLTVPSHYGGKGLGAVEYGIFCEEVAKASAAYIHNGHYQTEKMLLHYGTEE
                     QRGEFLPRLASGEWLAATAISERDVGSSFSGMASTARKEGKGYLLNGEKVHINDAAEA
                     QVINFFALAPRGLTVFLIDPASPGFWIAEKMDPIGLRASPIYSFRLEDYRVAEGRRIS
                     EEGQGVKVFFSAFNHSRIGNASCLLGIARAALEKAVAFGKSRMVGKNAVVDFQGIRWI
                     VAEVSTRLEAASLLRDKAAWMEDRGKDPARETSMAKLVAGEVSLEAVSQAIQITGSHG
                     CYRETPFERYWRDAKSLCIAGGTLEVMKNNIAKQVLG"
     gene            12270..12917
                     /locus_tag="VEL68_08150"
     CDS             12270..12917
                     /locus_tag="VEL68_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXZ35975.1"
                     /translation="MTAEVIQAFLSLSTPTISDALDLLRLSGGCLGLRPVVMGHKIAG
                     PAFTARFVPVGIGKKGAGDFLDDVPPGSVVVIDNGGRTYCTVWGDLMTRVAKRNGIAG
                     TVIDGVCRDVALIRELNYPIFTRGAFMVTGKGRVRLAAVQEEVTAGDISVHPQDLIIA
                     DDSGVLVVPKSRADEVLRAAQDIGAREDRIKRMVEEGMTLKEARAREGYDQIGKQ"
     gene            13015..13908
                     /locus_tag="VEL68_08155"
     CDS             13015..13908
                     /locus_tag="VEL68_08155"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HXZ35976.1"
                     /translation="MRQDLELLKGAIEIHVHSGPDLLPRLLDHAELVRMAKDAGYRAI
                     VLKSQNMGTADRVPFIRMLVPDIDVFGSVTLNYSVGGINPFAVNAALGFGAKIVWMPT
                     IDARHHMSYFGGLGQFGSSIKAEKKLPEFYKKAKGLTVLSDDGRLLPEVRDILDLIAE
                     SNTVLGLGHLSLKEMMALAKGCQDAGVRKMFIDHPDLAFTKVPLEAQLELTKLGVKMN
                     YVAAEISPRFYCISPKEIVNNMRQVGIKNSLISTDVGTPTNPNPIETMRSYIQILLDE
                     GLTPAEVKTMLHDNPAAFLYE"
     gene            14183..15211
                     /gene="dctP"
                     /locus_tag="VEL68_08160"
     CDS             14183..15211
                     /gene="dctP"
                     /locus_tag="VEL68_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ35977.1"
                     /translation="MDRKRALVAIGMAALLIGGGFLAFPSALFAQEKTIIWNVPHTAA
                     PSYYHILNLKLFAEKLKELSKGRMELRVHPASSLYPQNEQIPAVVDGRVEIAPILSGY
                     MVDMFLEMAVLDLPFMTRGLDEARAAADKMRPYYVERLAQKNIQLLAIGTWPTQQLFS
                     TKKAFAKVEDWKGAKIRVYGAETAELTKALGGAPVNIPFGEVYTALQRNVADGAITSA
                     TNAEPMKFFEVTKFINYWFISGAGIEFLGMNKKAFNALPKDLQPVVLDAVKQSRFEDK
                     EWEDAKAWDAKAKARCAELGLTIVDVPAAEIEKARKMVKPAWDNWLKRTGAEGQRALD
                     LAAKALGR"
BASE COUNT         3238 a         4431 c         4180 g         3491 t
ORIGIN      
        1 gtccggggat cgttcaccag catataatga agggtcggcg ccccactgat catggtggcg
       61 ctgtactttt cattggcttt gagaaactct tcgaagtcca gtttggcctg gataaccgcc
      121 gtggctccca gcatgaccac cgccagcata ttcacggtca gacccatggc atggaagaga
      181 ggggtcacgg acacgcggac catctcttcg tccaggttca gggccacaat gatccctttc
      241 acattggaat cgaagttccc atgggtaatc atggctcctt tgggaaagcc ggtagtgcct
      301 gaggtgtaga tcatggtggc tgtgtcatcg ggagcgcaat ccacggtcat ctcccgcacg
      361 ggcttttccg ccaggagggc ggaaaagagg agggagtccg gcagggtctg gtttccgatg
      421 acgatcatgt tcttcagccc tgggcgaatg gactggatga tctccccctg gctctggtgg
      481 acgatcagag tgttcacctc cgcattctcc agaatgtact tggattcccg ggccttcagc
      541 atgatattga ccgggacgaa gaccgctcct aacttgacca cggagaacca ggagacgacg
      601 aactccaggc tgctgggcgc aaagacggcc acgcggtctc ctttctccac tcccagcttc
      661 tgcaatccat gggcgaaggc ttccgtcagg tcttggagcg ctttgtatct ggtgggctgg
      721 tcgttaaaaa caaaggcgat cttatccggg agacgcttag cggtccgggg aagaatttcg
      781 gcaatattca tctcttttct cctcccgaaa tagagaaacg ctatgcgcta accactatgc
      841 ccatagcctt ttcaaatact caccggttcc ttgaactccc caaagacctt cttcaaagcc
      901 tcggtaatct ctcccacaga ggcgtaagcc ttgacagcct ggatgaaaca gggcatcagg
      961 ttttccttcg tttctgcaat ccggcacagc tcatccagcg ctttttgggc cttaagattg
     1021 tcgcgtgcag cctttaccgc tctcgtcctc tgaatctgcc tttccacgga tgtcgggtct
     1081 gcccggtgaa tgattagctg gcgcttcccc tcttccccgg cctggaattt attgactccc
     1141 acaacgattc tctctcccct ttcttcttcc cgggctacac gataggcctg ttcggcgatg
     1201 gccatctggg ggtatcccat ttcaatcgcc ttcaccgctc cccctatgga ctcaaccttc
     1261 tccatctccc ctttaatcgt tccttccaat tcctgggtga gagcttccac gaaataagac
     1321 ccgcccaagg gatcggctat tttggttact ccagtttcat aagctaggat ctgttgcgtc
     1381 ctcagagcca gccgggcggt gtgctctgtg ggaatggcgt aggcttcatc catggccggc
     1441 tggagcatcc cctgggctcc ccccaggatg ttggctagaa gaccataggc tccccggacc
     1501 agattgtttt ccggctcccg ggcccggtag gtacttcccc caaccgttcc ggtaaaacgg
     1561 aggagcatgg aattcgggtt gcgggccccg aatcgttcct tcatcatcct ggcccagaga
     1621 cgacgcaaag cccggtaccg cgctgcctcc tcgaagacct gcaatcctgt ggcaaagaag
     1681 aaagatatac gcggaccaaa atcatcaagg atcaaccctc gcttcagggc gtcctcgatg
     1741 taagcaacgg cattggcgaa agcgtaagcc ccctcctgga tcatgctggc cccggcttcc
     1801 cggaggatga ttccccggat gttgtatggg tagaagcggg gaagatggcg ggagcagaat
     1861 tcaacgatgt ccgtgctcat tcttagagcc gggccgggcg gaaagaccca ggtaccccgg
     1921 gagatatatt cgcagagcat gtcattggcc agggttccat tcagttcagc cagggggaca
     1981 ttttgcttct cggccaggac gaggtacatg gccaggagag cggcggccgg ggtgttgatg
     2041 ttgaaagaag aagtgatctg gtccagagga agcccgtcat acagaatttc catatcccgg
     2101 agagtgtcga tggccactcc cacctttccc acttcccctt gagccaaagg gtcatcggaa
     2161 tccaagccga tctgggtggg aaggtcaaag gcgcaggcca cgcccatgtt ccccgatttc
     2221 aaaaggaact tccagcgccg gttggtgtct tccgcagttc cgaacccgga atagaggcgc
     2281 atctgccagg ggcctttgcg atacattccc gggtagaccc cgcgggtgta agggtattct
     2341 ccgggaaagc cgatgtcgcg caggtaatcg atcccctgca gatcatcggg ggcaaaaacc
     2401 ggcgggaggg gccgacccat ctcagaacgg gccgagggca gctctccgat cttcttttcc
     2461 ttcgcccagc gcgtcttctc ttccgcgatt ttcttcaact catccataaa aaccccaata
     2521 ccttattcac cacggaggca cagagaccac agagacaaaa agcaaattat tagaataagt
     2581 gatgaattaa aaaaacctgg attattttgg cctcgaatct ctttttgatt ttttgtccga
     2641 tttctcggtg acctctgtgc ctctgtggtg aaaaagtcct ttagtagttc tcgtgagcga
     2701 acttttcaag ggactcccgg aaattcggat gggcgatggc cgtcaaggcc tcggccctct
     2761 cccgcagcgt ttttcccctt aggtcggcga ccccatattc ggttaccacg tattgaatgg
     2821 aatggcgggg atgggtgacg gccgagccgg gcggcaactg ggcaacgatc cgtgaatact
     2881 ttccatccgg agtcgccgag gtaacggcga tgattgactt cccttgggga gagaagagtg
     2941 ccccctggag aaagtcaaaa gagcccccga tggcactgat ctgtcttcct cccacggttt
     3001 cggcatttac ctgaccggtg aggtccacct cgatcgcccc caggagggag ataaactttt
     3061 cgatctgccc caccacccgg gaattgatgg tagtcatgat gggacgaccc tccaccatgg
     3121 gattctcgtg gacaaaatcg aagagctttt tgcttcccag aatctcggtg acgatcatct
     3181 tgggctgctg gtcgggactt atggacctct ccaccagccc cttctccaca caatccacca
     3241 tggcatcgat gcccatggca aaaaaacgca ggtcccgctt cccctccatg gattgaacca
     3301 cggcgtccgg gatcgacccg attccgatct gcagggtcgt cccatccgga atcaattcgg
     3361 cgacataatt cccgatcttc ctttctacct cccccacttt ttcggtcgac gggtattcaa
     3421 gcagaggacg ggaagtttcc actaggtaat caacctggga gagatggata aaggcatccc
     3481 ccagaacccg gggcatctgg tcattgacct cggcgataac cagtttggcc tccagcgccg
     3541 tgggcaggga gtggccgatg gaaacgccaa agcttagata tccgttttta tctggcggcg
     3601 agagttggac cagggccccg tcaattggcc agattccctt gcgggaaaag acaaagggtg
     3661 catccccctg gcgcatggga atgtacttca ccgtcccctt tttcagaagg tggcggatcc
     3721 ggggtgcgca ctgccaggtg cggaaggaaa aactttcctc cgtcccttcc tgtaaaaacg
     3781 ggtattcgat ctgcagaccg ctgacgatct ccaccccgcg cagccggtct ttccgggcga
     3841 tcaacgcctc caccagggtc tggggcaggc cgcagcacag gggaaggatc aaacgctcac
     3901 ccgaattcac cgcttccagg gcttcttcgg ccgaaaccca cttggaaaga cgcttcattt
     3961 tccctccgcc cccaggaatc caatcgcccg gccagtaacc gccttctctc cattctgttt
     4021 ctccaccgcc aggtcacact ccacggacca ttttctcccg gtcatttgga cctgcatgac
     4081 cgtcccactg atccggattt tgtcacccgg gcggatgggg gccacaaagc gcacgtcgag
     4141 gagttttcct ccctcggccc agcccatccc gaagcattcc atcatcagtt cattgaagaa
     4201 ggccaaggac aggggaccgt gggcgatggt gcccccgaag cgagtctgcc ctgcatattt
     4261 cgggtctaca tgaagtcgat taaaatcccc ggagatctta gcccacagca taatccgatc
     4321 ctgatcgatt tccttctcga tcaccggaag aggatcaccc gccttcgtct cccttaacat
     4381 tcttcgcatt cctcccttga aatcgaatcc tgaacctgga gatttttttt aacacggaac
     4441 cggaaacccg aaactcgcaa ccttcatttt ggccatatgg catacagccg tatggtactg
     4501 accggcattc ctttttgatc ctgcgcctcg atcaagaaag tcaccctttt tctcattttt
     4561 tcatcctgat agctttcgat caccttagcc tggactttca ggatgtccct gatcttaatg
     4621 ggattctgaa attcgaaccc ctgcttggcc agaaccccac ccgagggcat ggtgtggtcc
     4681 tgcaggtagg agagacgggc ataaatcgcc gccagccctg ggggggcgaa gccctcgtca
     4741 tacagttggt ggcggtcgcc caccgtctct atgaaatcct ggaccaactc cggggtgata
     4801 gggtactcca ggggtcgaaa gattcgcccg accggaaggt cagcaaataa cagctccggc
     4861 ttgtcttcct ggatttccat gaatgccacc tctttaaccg agactcatga aattcgaatc
     4921 tcgaattgcg aaacaaaatc aacattccaa tcggtttcgc tattcgattt ttggatttca
     4981 tgcgccttca atttttaaat agcttcggca attcaggccg atcccgaaaa ggtaagtctc
     5041 gcttgtcctt ccatgaagac gtccccattg gccctctggc acaggacttc tagggtccct
     5101 tcattttttc ccttctgaat ctccaggacc ttgaagcggg tgaccaggat gtctcttcct
     5161 tcaaattctc ctttcaccga gattccatcg gtgaaaatcc tcaggttttc cggaacggac
     5221 cagttcttgg ctaaagcttc acggatcaca tagccgacga gagcttgccc ggacagccct
     5281 tctttcatct taccggcaaa cagaagggcg ggggcaatct caccgttggg cctcaaatcc
     5341 tgaagagaaa ggggccggga gctgcagtgc cgcaccgggc cacttcgggt cacatggccc
     5401 aggagattcc ggccgcagaa ttcttcggcc atgagtcccg tatcgattac atcatcaata
     5461 ttgaggccgg tttgaacccc catctcttcc atcatgagca ccatgtcttc ggtgcaggtg
     5521 ttaccggcaa atcccttgtg gtacttgggc cgtcgggtgg ccggctgtcc ccccatcccg
     5581 ccgaaggagc tgtcgtgcag aacgatcccc tcctgaagag cggccagcgt gtttgccagc
     5641 cccattcccc gggtgtcgtg gaaatgggca acaaagtcca cccccggaaa acggtctttc
     5701 atatggccaa agatttccct gacctgccgc gggttggctt ccccggttgt gtctccgagc
     5761 atgatggagg tcgctccctt gtccaggtac cagccggtga gctcgatgat tttttccagg
     5821 ggaacctctc ccgccagggg gcatccgaag gcggttccta tgcagccgat gaccttgatc
     5881 ccggcctgcc gggcccgttt caccaggtct gccagaggag gcatagcctc ctcgaaggtc
     5941 cgttcgaggt tcgccaggag atgatcctcg ctggctgaca ggatggcagt aatttcttgt
     6001 accccataac cttgatggca gatgtccaga aggcggtcga gggccttatc gttgggaatg
     6061 atggcgacat aggtgacatc cgggggacgc cggattccct tcaagacttc tagagaatcg
     6121 gagaactggg ggatcaactt gggatgggca aaggaagtgg cttcgattcg cttgaaaccg
     6181 atctccacca tccggttgat gatggcaatc ttcttttccg taggaatgaa ctgaccctct
     6241 ccctggagcc cgtcccgggc ccagcattcg cagatgttta ccctctccgg catccctttc
     6301 atcgggtccc ttcctctcga tcaattgctc cccttgatac ttgcctttct aaaacgcagg
     6361 aaggcctttc cagaaattca gatcattcga aaacctggcg aattgtattg agcttgggcc
     6421 taccttgaga tcaaatcaga aattcgtgct tttccaaaaa agttgtcaga ttccttgccg
     6481 actggccgat gtgttcttcc atggccagcc ggactttcag tggttccccc cgatcgagag
     6541 ccttcaggat ttgttcgtga tcctcgttgc ttttttcccg ctggcccgga atggaaagaa
     6601 tgatccgcca ataacgggca aactggtccc ggtggttctt gattaaccca tgaagccgac
     6661 ggttttcgca tttatcgatg aagaactggt ggaaagcgat attcaacctt tgccactcgc
     6721 ccggatcttt ccgcacactc tttttcagcc gctcatggat gaaacgaacc tcccgtaggt
     6781 cctctttttt catcctctgc gcggcgaggc cgccggccag gccttccagc gcgccctgga
     6841 tcacatagag ctcttcgata tcctggaggg agaaacggga gacctcggtt cctttgtaag
     6901 gggtgatttt caccagcccc tccagttcga gctgcttgat tacctcgcgg atcacggtgc
     6961 ggctgacctt gagggattca gccagggagg attccaccag gcgggacccg ggcaggagac
     7021 ggcccgagag gacctgcctt cgaagatcgg cataaatggt ctctctcagg ggtctgttct
     7081 tctgggcgcg attcggagcg gacaaaggtc cctcctttgg tttgcaatat tgtttacaat
     7141 attgtttaaa atatgtcaag aagaaaagga acccttacat tgccctgttt tcattccctc
     7201 ctcccggtgc acctcctacc gaaaaaacca ggggaaccac ccggctttca attccactta
     7261 ttcgattggt tattcctcct tttcgtagat ttttttggcg atggagggga gctgtccgca
     7321 gtttggactc ttcttgcccc tcctcctttc ccttcagtat ttgacggtca tcccgccgaa
     7381 ctgtttcctc agttctctcc gcagaacctt gcccaccgcg cttcggggca acccccctac
     7441 aaaggccacc cgtttgggga ctttgaactt agccagatgt tgagaggcgt attcgatgac
     7501 ctccgcctcg ctcagcgact ggccggtctt gggaacgacc accgccatca ccgcctcgcc
     7561 gaactgggga tcgggaatgc cgatgcagga ggcctcctgg attttgggat gggcgaaaag
     7621 gacctgttcc acttccgccg gatagatgtt ttctccgcct gatatgacca tgtccttctt
     7681 gcggtccttg atatagagat atccctctcc gtcgaacctc cccatatcgg ccgtgtggaa
     7741 ccagcccccg gcgaaagcct cggcgttggc ctccgggttc tcgaaatact cgctgaacag
     7801 ggctggactc cgaacgcaga tctcgccgat ttctccggca ggcagctccc gtctgctttc
     7861 gtccaagatt ttcacttcgg tgaggggatg gggtttgccc acggtggtcg cgttgagcac
     7921 ctttccctta gtcacatgca tggcgatggt ggcgatctcg gtcatcccgt aattgtcgca
     7981 gatatccacg cgaggaaagg ttttggcaaa cttctctttc agggccacgg gaagcggagc
     8041 ccccgtgcag cggacggtgt gccaggagct tagatcatac ttctccaggt tggggacctg
     8101 gaggatggcc gcggccacga tgggaaccat ggcgaattgg ttcaccttct ctttctggat
     8161 cgtctccatg agcttggccg cgtcgaaagc cctcagaatc acgcaacggc cgaatttaat
     8221 cagctggctt accgctgtga tgtaggcggc cttatggaag aaaggcaggg ggtaggccat
     8281 gcattcctga atgttgaacc gtccaagcat ggtgtcgtag aggatggagt tcaccagcga
     8341 ccacatattg ctcttgtggc ggcagaagac ccctttggga aatcccgtgg tccccgccgt
     8401 gtacatgagc atggccgggt catcggggcc gatgggaagg ccggggtggc gggaagaatg
     8461 acgggccacc acctcttcgt aaagatgcat atctcccggg gcgtgttttc ccacgacaat
     8521 gtagttttga atggtgggca tttcggaacg aagtttttcc accaccggaa ggaactcttc
     8581 gccgaagaag agggccttgg ccttggcgtg gttgaggatc caggcgatct cccgcgggga
     8641 gaggcggaag ttgatcggaa ccgggagggc tccggctttg ttcgaggcca tatagttttc
     8701 caggatctcc ggacagttag ccagaaagga ccctactcga tcccccctct ggatccccat
     8761 ttccacgagc gcgttggcca aggagttgac ccggtcatcc acctccttga aggtccggcg
     8821 gacttcgtcg cagatcagaa actcccggtc ggggtacttt ctggcagcca gctccaaaag
     8881 ggaaacggtg gtcacctctt cgggacggta ttccatgcct tcctccccgg gtttgtacag
     8941 acctacttta agcggactcg aactccgttt tttaaaccct taaagaaggg agaattcgga
     9001 ttcctatcct tctccagctt cttcttcctc cctcgaatat ttgaccagag gaaaaatcaa
     9061 tttgccccga aagcagaggg gagaaggttt caggagaggc gcaagatggg atgacgggaa
     9121 ttccaggagt ggtgccggtt ggtgcctgtt accataccca tcagagttcg gggattccat
     9181 gaggccttcc tccctccatg ccgtgggtag agcacagcgc gggctttttg aattcgattt
     9241 gattttacat ggcggggtgt ttttgtcaac gggattttat tgagccctct cggccaagtc
     9301 gatcccaggc ggacccgatt cccctggatt tcagggatgc cttttggact ttctccgagg
     9361 cggtcttggg aagggaggaa acaaattcca gatagcgggg gatctggaac ttcggcaact
     9421 tttcttgaca tctttcgatc aactctcgcg gttcgaccga tcgtccttca tgcaaaacca
     9481 catagacttt gatttcttcg cccaggatcg ggtccggcac tccaaccgcc gcggcttcgg
     9541 ccaccgcggg gtgatccgtg aggactttct cgatttcata gggggtgata ttctccccgc
     9601 cccggcgaat gctctctttc ttccggccgc agaaatagaa atacccctcc gaatcgacct
     9661 tccccaggtc tccggtgtga aaccacagat tccggtaatc cctgagggtc tcctccggca
     9721 tcttatagta tccattgaac ataacgaaag gcttcttcgg ccgtacgacg atttcgccaa
     9781 cctggcccgg agggacctca tcatcctcat catcaaagac cttaacctca aagcattccc
     9841 tgacttgccc caccgaaccc gatttgatct ttcccggccg gttgatggtg ttccaagttc
     9901 cggtttccga catgccgtat aattcgcaga cctggagccc gaaacgccgg gtcgctgttc
     9961 cgtggacctc cttcggggcg ccgccgccga aacattttct ggcgggggac cccttttcct
    10021 cttccctttc tggctggttt agaagaatgg gcaggacagc ccccacgtag ctgaaggctg
    10081 tggcccggta agctcgaatc tcctgccaaa accgggaggc gctgaaccgt tccgccaggg
    10141 caatcgatcc ccccgtaacc agtgcggtga gcacgcagta gacttggggg ttggcgtggt
    10201 aaagggggag gcaagtgtaa atgatgtcct cgggtttcat ttccacataa tcgcgaaggt
    10261 cctcagccgc ggcgatgtag gctcggtgag ggttgagcac ggccttgggc catcccgtcg
    10321 ttcccgaggt ataagtgatg atcaggggat cccccccctt gggaggcccc ccctcgacgg
    10381 attctgaaga aatccctgct aagagacggg ataggctgtg agctccctcg atttcgaccc
    10441 ctccttcggc caggtcaaaa accacgaggg tcagtcccgg agccttccct gctttctcga
    10501 actcgtcctg gaactccgcc cccaccaggc agtattttac gtccgcctgc ctgagggcat
    10561 attccagccc gtccgccttc cattgggtat tgaaggccac tagaaccgcc cctaatttcg
    10621 ccaacccgaa ccagcaggcg atccattccg ggcagttggg gagcatgacc gccacgtgat
    10681 cccctttgcg gacaccgagc tcgcgaaaaa cccggctgaa ccggttgctg aggaggtcca
    10741 attccctgta ggttaattgg cggtcgcgaa aaaagagata ctccttatta ccccgcagcc
    10801 cgaccatttc ggtcagaagg gttttcaagt tatccatttc taccccagga cctgtttggc
    10861 tatgttgttc ttcatcactt ccagggtccc tcccgcgatg cagagcgatt tggcgtcccg
    10921 ccaataccgc tcgaagggag tttcccggta gcatccatgg ctgccggtaa tctggatggc
    10981 ctgggaaacc gcctccaggc tgacctctcc ggccaccagc ttggccatgg acgtttccct
    11041 ggccgggtct tttccccggt cctccatcca ggccgcttta tccctcagga gggaggcggc
    11101 ttccaggcgg gtgctcacct cagccacgat ccagcggatc ccctggaagt cgaccaccgc
    11161 attctttccc accatccgac ttttcccgaa ggcgaccgct ttctccaggg ctgcccgggc
    11221 aatccccaga agacagctgg cgttcccgat ccggctgtga ttgaaggccg aaaaaaatac
    11281 cttaactccc tgcccttcct cgctgatcct tcgcccctcc gccacacggt aatcttccag
    11341 gcggaaggaa tagatcgggg aagcccgcaa cccaatgggg tccatcttct ccgcgatcca
    11401 gaagccgggg gaggcggggt ctatcaggaa aacggtcagc ccccgggggg ccagggcgaa
    11461 gaagttgatg acctgggctt cggctgcgtc attgatatgc accttctctc cattcaggag
    11521 gtagcccttt ccttccttcc gggccgtgga agccatgccg ctgaaagagg aacccacatc
    11581 ccgttccgag atggccgtcg ccgccagcca ttctccgctg gccagccggg gcaagaattc
    11641 cccccgctgt tcttccgtcc cgtaatggag gagcatcttt tcagtctggt aatgcccgtt
    11701 gtggatgtag gctgcgctgg ctttggcgac ctcttcgcag aaaatcccat actccacggc
    11761 ccccaagccc ttcccgccgt aatgggatgg gactgtaagc cccaaataac cttcccgccc
    11821 cattttccga aaggtctccc gggggaagag gcctttttca tcatactccc gcgcctcggg
    11881 ctcaatgacc gtttcggaaa aagcccgcac cgaggcctga aacgctttct gggcctcact
    11941 catttcaaag tccaccgtct ttcctcctgt tgcggggtca taaagaaaga ttatatggat
    12001 gttggagctt acaatattta ctaaattccc tttcctgtca acccatgtct gtttacaaca
    12061 tacggataaa cctttttccc ttgacaagaa aaggatcatc tagtattcac aagagaatac
    12121 ggcccgccgg gttcgcgcac ccaagacctc catcttgcaa gcggaagcca gcgtccaccc
    12181 cgactaggcg ggtagaagat tgcgccgatt atactgcgtt caaaggattc ttgccgcacc
    12241 tggccgtcgc gcaacaagga ggatttgcca tgaccgcgga ggtgatccaa gccttccttt
    12301 ctctcagtac tcccacgatt tcggatgcct tagacctgct gcgcctgagc ggtggatgcc
    12361 tgggcctgcg acctgtggtc atgggccata aaattgcagg cccggccttt actgccaggt
    12421 tcgtacccgt cggcattgga aagaagggcg ccggtgattt cttggacgac gtgcccccgg
    12481 gaagcgtagt ggttatcgat aatggagggc gaacgtattg cacggtttgg ggtgacctga
    12541 tgacccgggt ggcgaagcgt aacggaatag caggcacggt catcgacggc gtatgccggg
    12601 acgtggccct catccgggag ctgaattatc ccatcttcac ccgcggcgct tttatggtga
    12661 ccgggaaagg gcgggtgcgg ctcgccgcag tgcaggaaga ggtgaccgca ggagatattt
    12721 ccgtccaccc tcaggatttg atcatcgctg acgacagcgg cgtgctggtg gttcccaaat
    12781 ctcgggcgga tgaggtgctg cgggcagctc aggatattgg cgctcgggaa gaccggatta
    12841 agcgcatggt ggaagagggc atgaccctaa aggaagctag agcgcgggag ggatatgatc
    12901 aaataggcaa acagtaggga atcaggacat agccgaccca ccggaaataa acgaatgggc
    12961 gaaaataacg attaaatctc aaagcgggga aagccggaga aggaggagga ggaaatgcga
    13021 caagacctgg agcttctcaa aggggcgatt gaaatacatg tccattcggg tcccgattta
    13081 ttgccaagac tgttggatca cgccgaattg gtacggatgg caaaagacgc gggttatcgt
    13141 gccatcgtgc tcaaaagcca gaatatgggg acggccgatc gagtcccttt catccgcatg
    13201 ctggtgccgg atatcgatgt ctttgggagt gtaacgctga attactccgt gggggggatc
    13261 aacccttttg cggtaaacgc cgccctgggc ttcggggcca aaatcgtgtg gatgcccacc
    13321 atcgacgccc ggcatcacat gagttatttt ggaggattgg gacaattcgg gtcatccatt
    13381 aaggcggaaa aaaagctgcc ggagttctac aagaaggcca agggattgac cgtcctttcc
    13441 gatgatggca ggctccttcc cgaagtgcgg gacattttgg acttaattgc cgagagcaat
    13501 accgtattag gcctggggca tttgagtctc aaggaaatga tggctctggc caagggatgc
    13561 caggatgccg gcgtgagaaa gatgttcatc gatcatcctg acttggcctt caccaaagtt
    13621 cctctggaag cgcagcttga gttgaccaaa ttgggagtca agatgaacta cgttgcggcg
    13681 gagatctccc cccggttcta ttgcatttcg cccaaggaga tcgtgaataa catgagacag
    13741 gtgggaatca agaactcatt gatttccacc gatgtgggaa cgccgacgaa tcccaatccc
    13801 attgaaacga tgcgttccta catccaaatc cttctggacg aaggattgac ccccgccgaa
    13861 gtgaagacca tgcttcatga caatcccgcg gctttccttt acgagtgaaa gaaagcgccc
    13921 agggcccggt ttcgagaggg atcggggcga gaaggtttgg atgggcatat aattttttct
    13981 tgacggcccg aagctgaaat gggattttag gcgatatttt caccattagg ggtcctttgg
    14041 gatctgggtt cttcggagac tcttctccct gaaagagggg ttcagcaccc ccgccgctgg
    14101 gcatggcagg tttgaacggt cgaaggggcg gtggctcgcg gccgccgcgc tcttcctaat
    14161 taatgaggat ggaggtggaa ggatggacag aaagagagct ttggtcgcta tcggcatggc
    14221 ggcattgttg atcggggggg gattcctggc cttcccttca gcccttttcg cccaggagaa
    14281 gaccatcatc tggaacgtcc cgcatacggc cgcacccagt tactatcaca ttctcaacct
    14341 gaaactgttc gccgaaaagt tgaaggaact gagcaaggga cggatggagc tccgggtcca
    14401 tcccgcttct tccctttacc cgcagaatga acagatcccg gcggtggtgg acggaagggt
    14461 ggagattgct ccgattctgt cggggtacat ggtggacatg ttcctcgaaa tggccgtcct
    14521 ggacctgccc ttcatgaccc ggggtctgga tgaagcccgg gcggcggccg acaaaatgcg
    14581 gccctactac gttgaacggc tggcccagaa gaacatccag ctcctggcca tcgggacctg
    14641 gcccacccag cagcttttct ccaccaagaa agccttcgcc aaggtggaag actggaaggg
    14701 ggccaagatc cgggtctacg gcgccgagac ggcggaactg accaaggccc taggaggggc
    14761 gccggttaat atcccctttg gcgaggttta tacggccctg cagagaaacg tggccgacgg
    14821 agccatcacc tcggccacca atgccgagcc tatgaaattc ttcgaggtga cgaagttcat
    14881 caactactgg ttcatcagcg gcgcgggaat cgaatttctt ggcatgaaca agaaggcctt
    14941 taatgccctg cccaaggacc tgcagccggt ggtcctggac gcggtcaagc aatcccgctt
    15001 cgaagacaag gaatgggagg acgccaaggc ctgggacgcc aaggccaaag cgcgctgcgc
    15061 cgagctgggt ttaaccatcg tggacgtccc ggcggcggag attgagaaag cccggaagat
    15121 ggtcaaaccc gcctgggaca actggctcaa gcggaccggc gccgaggggc aacgggccct
    15181 ggacctcgcc gcgaaagccc tgggacgcta gtccccgcgg aacaacgttg ccgtccagct
    15241 tttttcccag cagttgagag cgtaacccgc agcgcgatta cactccgtca cggccgtagg
    15301 ggcatcccgc caaaggcggg atgcccctac gaaaaagaat
//
LOCUS       DASPRR010000212         7344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1017483, whole genome shotgun sequence.
ACCESSION   DASPRR010000212 DASPRR010000000
VERSION     DASPRR010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017483"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1031)
                     /locus_tag="VEL68_08165"
     CDS             complement(156..1031)
                     /locus_tag="VEL68_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ35978.1"
                     /translation="MTVGDIKKICVVGAGNMGHQISLLCAIHGYKTTCTDVVPEVLKK
                     AEKFADTYLPGRVEKGKMTKEVAEAARKNISFSQDLKAAAKDADFVIEAVLEILDVKR
                     KLFAQLDEVAPAHAILATNSSYIVSSLIADATKRPAKVVNMHFFNPALVMKLVEVVKG
                     PHVSDETAKITYDLSEKLEKIPVLLKKEVEGFLLNRIFRVIGREALWMLEMGVASFED
                     IDKACVYGAGHPMGPFRLMDLTGIDLNYIMSLERFKKTGDRSDLPSPSVVERYLKGQY
                     GEKTGKGWYDYTQKK"
     gene            complement(1133..2335)
                     /locus_tag="VEL68_08170"
     CDS             complement(1133..2335)
                     /locus_tag="VEL68_08170"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015233122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXZ35979.1"
                     /translation="MKEVVIAGYLRTAQSRSRPNDPPRDWFHKVRSDDLLARLLPEVV
                     KRTGIKPEEVEDFIVGSAIGVTEQWSYGGRFPIFLANFPNTIPAKFVDQQCGSAMAGI
                     HIGFMEIAMGFADIVMVGGMEHMTRVPMGSLAGEKGAISPNLRLFTDPAYKHWEMMTT
                     MNMGLTAEKLFGMSGLTKGDMDKWGVRSHQLAAKAQKEGFFKDEILPIEAEQGDGKMM
                     VVDRDQAVRGDTTLEQMKDLKPAYRPDGNITAGNSSPLNAAATSMILMSKETAKKKGI
                     KPLATIKSIGFAGVDPSIMGAGPVPASKRALKAAGLQVKDIDYWEINEAFAIVPLYCI
                     KELGLNPDRVNVMGGGLAIGHPLGATGVRLTGTLARILQSKKARYGLANACVGGGQGV
                     ATIIEREA"
     gene            complement(2495..3991)
                     /locus_tag="VEL68_08175"
     CDS             complement(2495..3991)
                     /locus_tag="VEL68_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ35980.1"
                     /translation="MRTKEQYIERLRTMRRNIYMNGEKIARDDEVQMPTINTIGITFD
                     YAADPKHEGLCTAKSHLTGEKINRFNHIHQSKEDLHKKQDMTRLLCQVAGGCIQRCMG
                     IDGTNAIYNVSYEADKMNNGATQYHQNFKKWVERFQKEDLVGCCAQTDVKGDRMKRPS
                     QQKDPDMYVHVVERKKDGIVVRGSKVHNSEASVADEIVVVPTRALLPEEKDWAVAFAV
                     PGDWEGVKQVVSIHNLRDRQYFKRGFTPGATDSYTIFDNCFIPWERVFLCGETIHGGV
                     CALLFALFHRHSYSGCKPALGDLMLGTVALAADYNGIGKASHVRDKLAEIIRVSELGY
                     AAGFTASEMGKPEVYIPGVGFRPYGPGSYIPNSIYANVGRCLTGEAVFREAEILCDVS
                     GGIPATFPYEGDFMNPETKDLLYKYITRNPDVHPEDAAQLWRYVGDLACSATGGIHNY
                     GAFHGGGSPIMEAIAITSQYDIEARKKLVKKLAGIQDRKVNPENKDKK"
     gene            complement(4178..4936)
                     /locus_tag="VEL68_08180"
     CDS             complement(4178..4936)
                     /locus_tag="VEL68_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXZ35981.1"
                     /translation="MKISELVQRTHVSKETVHYYIREGLLPKPRKRGRNIADYDDGYV
                     ERIRLIKELQDRYFLPLAVIKNILKHQKKSPEGQSLLSLRREYFRPVDQLLPNEVEGE
                     EAFLRATELGGKWLAKMEEWGIISPEIRDGRKVYSQDDITLGKLVLEMDKVGIGVRNG
                     FDPEALKHYRDMFREIVVMSHKYYFEATLGKLPPEVFSKRIVQGREIMSVFFYHLYRK
                     LSREEYRRILRLLEAEPKEGEDFSGVPRGVSEGL"
     gene            complement(4991..5701)
                     /locus_tag="VEL68_08185"
     CDS             complement(4991..5701)
                     /locus_tag="VEL68_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35982.1"
                     /translation="MLKLSNLSTLIGGFQAVREISLEIPQGDFVTLLGSNGAGKTTLF
                     RTIAGVLKPFQGTIEFKGRQIQGMPAHRVVKLGLCLCPEGRLLFPQLSVLKNLTMGAY
                     PRRKDRQGVQKSLEQVYALFPILRDRKQQLAGTFSGGEQQMLAIARALMSRPELLLLD
                     EPSMGLAPLVVRKIIGEVVKAIHEAGTTIFLSEQNARMALMIARRGYVLETGRIVLEG
                     SNSELMSDEKVKKAYIGA"
     gene            complement(5694..6461)
                     /locus_tag="VEL68_08190"
     CDS             complement(5694..6461)
                     /locus_tag="VEL68_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ35983.1"
                     /translation="MLLEVKNLTKNFAGLAAVSNVSLHVKEGEILAIVGPNGAGKSVL
                     FGLISGLLRPTSGVIHLQGEEITGLRPHRIAQKGIARTFQTTALFDQLRVVDNLAIGY
                     RLRTQGGFWNTLLHTRLWKEDKDRTTAKVMEALSFIGLAGKAFHFVSTLSQAEQKRLS
                     IGVALISQPKMILLDEPTGGLILEDTDEITGLIRKIHKVAGITVCLIEHKMRMVMDLA
                     DRMVVLNFGSVIAEGTPEEISNNPEVIEAYLGGESIA"
     gene            complement(6466..>7344)
                     /locus_tag="VEL68_08195"
     CDS             complement(6466..>7344)
                     /locus_tag="VEL68_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ35984.1"
                     /translation="IMGCLFSIATLSMNLILGYTGQASLAHAGFFGIGAYGVALLTKG
                     GLSFWLALPLSALLAAAIGFLIGLPTLRSRGSYFAISTMCFGVIISIVAGNWIDFTGG
                     YNGIVGIPLPNPLSIPLIGDLGFQTQVGQYYLVLAFLFLTLLIMHRLVNSLLGLSFMA
                     IRNNEVLAGSVGINTFAGKILSFVVANFFAGMAGGIYASLMGTISPSTASLDLTFNWL
                     IFLLLGGAATLAGPIIGAFVIPVIMEYLQFLAEYRLIIFGALLIVVIIYFPRGLMGGF
                     TTLQQRVRGLYAQRRG"
BASE COUNT         1459 a         2144 c         2074 g         1667 t
ORIGIN      
        1 aagaagaagt tgataactga attgcctacg gggggaattc ccgttctaag gaacgacaaa
       61 acttcccttt cccagggggt cgaaaccccc tggttccggg caaagaaaag agtctctccg
      121 ggcaatccat ggaggagcag gcaaaaggaa tggctttatt ttttctgagt gtagtcgtac
      181 cagcccttgc cggtcttttc cccgtactgg cctttcaggt acctttctac cacactgggg
      241 gaggggaggt cggaccggtc gccggtcttt ttgaaacgct ctagggacat gatgtaattc
      301 agatcgatcc cggtcaggtc catcagccgg aacgggccca tggggtgccc ggcgccgtag
      361 acgcaggctt tgtcgatgtc ctcgaagctg gccaccccca tctccagcat ccagagggcc
      421 tcgcggccga tcacccggaa gatccggttc agaaggaatc cctccacttc ttttttgagg
      481 aggaccggaa tcttttccag cttctcggag aggtcatacg taatcttggc cgtctcgtcg
      541 gaaacgtggg ggcccttgac cacttccacg agcttcatca ccagggcggg gttgaagaag
      601 tgcatgttca ccactttcgc cggccgtttt gtcgcgtcgg cgatgaggga gcttacgatg
      661 taggagctat tggtggccag gatggcatga gccggagcca cttcgtcgag ctgggcgaag
      721 agcttccgtt tcacgtcgag gatttccaaa accgcctcga tcacaaaatc cgcgtccttg
      781 gccgcggctt taaggtcctg gctgaaagag atgttcttcc gggccgcctc cgccacttcc
      841 ttggtcattt tccctttttc gaccctgccg ggcaggtagg tatccgcaaa tttttctgcc
      901 ttcttcaata cctccggaac tacgtccgtg caggtcgttt tgtagccgtg aatggcgcag
      961 agcagggaga tctggtgccc catgtttccc gcgcccacta cgcagatctt tttgatgtca
     1021 ccaacggtca tatcaggcct ccttaaagag cgatcagcgg tcagcgctca gccggcagcc
     1081 aacgtccttt tatccttcct ttgccgaacg ctgatcgctg attgctgtag ttttaggctt
     1141 ctctctcaat aatcgttgcc acaccctgcc caccgcccac gcaggcgttg gccaggccgt
     1201 agcgggcctt cttggactgg aggatgcggg ccagagtgcc cgtcaggcgg acgccggtcg
     1261 cccccagggg atggccgatg gccaggccgc cgcccatgac gttgacccgg tccggattga
     1321 gacccagctc cttgatgcag tagaggggaa cgatggcaaa agcctcgttg atctcccagt
     1381 agtcgatgtc cttcacctgg agcccggcgg ctttcagcgc ccgtttactg gccgggaccg
     1441 gaccggcgcc catgatggag ggatctacgc ccgcgaagcc gatggacttg atcgtggcca
     1501 ggggcttgat gcccttcttc ttggccgttt ccttggacat gaggatcatg gatgtcgcgg
     1561 ccgcattcag gggagaggag ttcccggcgg tgatgttgcc gtcgggtcta taggccggct
     1621 tgaggtcctt catctgctcc agggtggtgt ctccccggac ggcctggtcc ctgtccacca
     1681 ccatcatctt cccgtcgccc tgctccgctt cgatgggaag aatttcgtcc ttgaagaaac
     1741 cttccttctg ggccttagcg gccagctggt gggagcggac tccccacttg tccatgtctc
     1801 ccttggtcag cccgctcatc ccgaagagct tttccgcggt caggcccatg ttcatggtgg
     1861 tcatcatctc ccagtgctta taagcagggt cggtgaaaag ccgcaggttg ggggagatgg
     1921 cccccttttc cccggccaga gaacccatgg gaacacgggt catgtgttcc atgcctccga
     1981 ccatcacgat gtcggcgaag cccatggcga tttccatgaa accgatatgg ataccggcca
     2041 tggctgaccc gcactgctgg tccacgaatt tcgccgggat ggtattgggg aagttggcca
     2101 gaaaaatggg aaagcggccc ccgtaactcc actgttcggt gaccccgatg gccgatccca
     2161 caatgaaatc ctccacctct tctggcttga ttcctgttcg cttgaccacc tcgggaagca
     2221 gcctggccag aagatcatcc gaccggacct tgtggaacca gtccctgggg gggtcattgg
     2281 gtctggacct ggactgagcc gttctcagat atcctgcgat gacgacctct ttcatatgca
     2341 gattcctcct tgtaaataag atttatttag ccgacgaatc tccgaaatcg tccccatccc
     2401 ccctttatta aaggggggca aggggggatt tccggcaggg gcgatgttct ggaaagaaca
     2461 taacagtagg ttgcggctcc ggcagctctg tgttttattt cttgtccttg ttttccggat
     2521 ttaccttccg gtcctgaatt ccggcgagct tcttgaccag cttcttccgg gcttcgatgt
     2581 catactggct ggtgatggca atggcttcca tgatgggcga gccgccgccg tggaaggcgc
     2641 cgtaattgtg aattcctccg gttgccgaac acgccagatc gcccacatac cgccacaact
     2701 gcgccgcatc ctcgggatgg acatccggat ttcgggtaat gtatttgtac aggaggtcct
     2761 tggtctcggg attcatgaag tccccttcgt aggggaaggt ggccggaatg ccccccgaga
     2821 catcgcacag gatttcggct tcccggaaga ccgcctcgcc ggtcaggcag cggcccacgt
     2881 tggcgtaaat ggagttggga atgtagctgc ccgggccata ggggcggaag cccaccccgg
     2941 ggatgtagac ttcgggtttt cccatttccg aggcggtaaa gccggcggca tagccgagct
     3001 cggagacccg gatgatctcc gccagcttgt cccgcacgtg ggaagccttg ccaatgccgt
     3061 tgtaatcagc ggccagggct acggttccca gcatcaggtc gcccagggcc ggtttgcagc
     3121 ccgaatagct gtgccggtgg aagagggcga agagcagggc gcatactcct ccgtgaatcg
     3181 tttcgccgca caggaagacc ctttcccagg gaatgaaaca attgtcaaag atagtataag
     3241 aatcggtcgc ccctggggta aatcccctct tgaaatactg acggtcgcgt aagttgtgga
     3301 tggagacgac ctgcttgacc ccttcccagt ctccggggac ggcaaaggcc acggcccagt
     3361 ccttctcttc gggcagaagg gcgcgggtcg ggaccaccac gatctcgtcg gccacggaag
     3421 cctcggagtt atgcaccttg gaaccccgga ccacgatccc gtccttcttc cgttccacca
     3481 cgtgcacata catgtccggg tccttctgct ggctgggccg cttcatgcgg tcccctttca
     3541 cgtcggtctg agcgcagcag cccaccaggt cttctttctg gaacctctcc acccattttt
     3601 taaaattctg gtgatattgg gtggccccat tgttcatctt gtcggcctca taggagacgt
     3661 tgtaaatggc gttggtcccg tcgattccca tgcaccgctg gatgcaaccc ccggcgacct
     3721 ggcagagaag ccgggtcatg tcctgcttct tgtgcaggtc ctccttgctt tgatggatat
     3781 ggttgaagcg gttgatcttc tctccggtca ggtgggattt ggcagtgcac aagccttcgt
     3841 gcttggggtc ggcggcgtag tcaaaggtga tgccgatggt gttgatggtg ggcatctgca
     3901 cttcgtcgtc ccgggcgatt ttttcgccgt tcatgtagat gttccggcgc atcgtccgca
     3961 gcctttcgat gtactgctct ttggttctca tttcttcctc ctccttcctc cttcatttga
     4021 acttggttta tacacctctg aattcccccg aaataaatgc atcttaccgc tcagcacccc
     4081 tctagggcca tgagaaacct catatcctcg ctcaagcttc ccaatcgcta taccatgccc
     4141 gttttcgggg acaattgtca agaccttgtg gggttattca aaggccttcg gaaacgcccc
     4201 gaggaacccc ggagaaatcc tccccttcct ttggttccgc ttccagcagc cgcaagattc
     4261 tccggtattc ttcccgggaa agtttgcggt aaagatgata gaagaaaacg ctcatgattt
     4321 ctcttccctg gacgatcctc ttggagaaca cctccggggg aagcttcccc agggtggctt
     4381 cgaaatagta tttatgggac atgaccacga tttcccgaaa catgtcccgg taatgtttga
     4441 gagcctcggg gtcaaaccca ttcctgaccc cgatgcccac tttatccatc tccagaacta
     4501 atttgccgag ggtaatgtca tcctgggagt agaccttgcg cccgtcgcgg atttccgggc
     4561 tgatgatccc ccattcctcc atcttggcca gccacttccc ccccagttcc gtggccctca
     4621 ggaaagcctc ttccccctcg acctcattgg ggagaagctg gtccacgggc cggaagtatt
     4681 cccttcgaag gctcaggagg gattgtcctt cgggggattt tttctggtgc ttgaggatat
     4741 tcttgatgac cgccaggggc agaaaatagc ggtcctgaag ttcctttatc aggcgaatgc
     4801 gttccacata gccatcatcg taatcagcaa tgttcctgcc ccgtttgcgg ggcttgggga
     4861 ggaggccttc gcggatgtaa taatggacgg tttctttgga gacgtgggtt cgctgaacca
     4921 gctcactgat tttcacgagt tcctctccgg tcgggtgggg ttagcgaata ggggcgtcat
     4981 cgaaccctcc ctacgccccg atgtaggcct ttttcacctt ttcatcgctc atgagttcag
     5041 agttcgatcc ttccagcacg attctcccgg tttccagcac ataaccgcgc cgggcgatca
     5101 tcagggccat gcgggcgttc tgctcggaca aaaagatcgt cgtccctgcc tcgtggatgg
     5161 ctttgaccac ctctccgatg atcttccgga cgaccagggg ggccagcccc atggaaggct
     5221 catccagcag gagcagttcc ggccgggaca tgagcgcgcg ggcgatggcc agcatctgct
     5281 gttcgccccc gctgaaagtg ccggccagtt gttgtttccg gtcccgtaag atgggaaaca
     5341 gggcatagac ctgttccagg gacttctgga ccccctggcg gtctttgcgg cgcgggtagg
     5401 cccccatggt caggttcttc aacaccgaga gctggggaaa aagcaggcga ccctctgggc
     5461 agaggcacaa ccccagtttc acaacccgat gggccggcat tccctggatc tggcggccct
     5521 tgaactcaat cgtcccctgg aacggcttga gcacgccggc aatcgtccgg aagagggttg
     5581 ttttgccggc gccgttgctt cccaacaggg tgacaaaatc tccttggggg atctccaggg
     5641 aaatttcccg aactgcctga aacccgccga tcagggtgga taggttggaa agtttaagca
     5701 atggattccc cccccaggta ggcttcgatc acttcgggat tgttgctgat ctcctccggc
     5761 gttccctcgg cgatcacgct tccgaaattc aggaccacca tccggtcggc caggtccatt
     5821 accatgcgca tcttatgttc gatgagacag accgtaatgc ccgcaacttt atggatcttc
     5881 ctgatcaagc ccgtgatttc gtccgtatct tccaggatta agccgccggt gggctcgtcc
     5941 agcaggatca tcttgggttg gctgatcagc gctactccga tggagagacg cttctgctcc
     6001 gcctgggata gggtggagac aaagtgaaag gccttccccg ccaggccgat gaagcttaaa
     6061 gcctccatta ctttagccgt ggtccgatcc ttatcttcct tccagaggcg ggtatgcaga
     6121 agggtgttcc agaagccacc ctgggtgcgg agccggtagc cgatggccag gttgtccacc
     6181 acccgcaatt ggtcgaaaag cgcggtagtc tgaaaggtcc gggcaatgcc tttttgggcg
     6241 atccggtgcg gcctcagccc ggtaatttct tctccctgca ggtggatgac cccggaggtc
     6301 gggcgcagca gcccgctgat caggccgaat agaaccgatt tcccagcccc attgggtccg
     6361 acgatggcca ggatctcccc ctctttaaca tggagagaca cattgctgac ggccgccaag
     6421 ccggcaaaat tcttggtgag attcttaacc tccaataaca cccttctatc ctctcctttg
     6481 agcgtacaag cccctcaccc tttgctgcag ggtagtgaat ccccccatca gtccccgggg
     6541 aaaatagatg atgactacga tcagcagggc tccgaaaatg atcaagcggt attccgccag
     6601 aaattgcagg tactccatga tcacgggaat cacgaaggcc ccgataatgg gacccgccag
     6661 cgtcgccgcg ccccccagga ggagaaagat aagccagttg aaggtcaggt ccagcgaggc
     6721 cgtgctgggg ctgatcgtgc ccatcaagct ggcgtaaatt ccgcccgcca tcccggcgaa
     6781 gaaattggcc accacaaagg acaaaatttt cccggcaaag gtgttgatcc cgactgaccc
     6841 ggccaggacc tcattgttcc ggatggccat aaaactcaat cccaggaggg agttaaccaa
     6901 gcgatgcatg atcaataagg tcaaaaacaa gaaggccagg accaggtaat attgacctac
     6961 ttgggtttga aaaccgaggt ctccgatcag gggaatggaa agcgggttgg gcagagggat
     7021 ccctacgatg ccgttgtatc cgccggtgaa gtcaatccag ttgccggcca cgatcgaaat
     7081 aatgactccg aagcacatgg tggaaatggc aaaataagac ccccgggagc gcagcgtggg
     7141 cagtccgatc aagaaaccga ttgcggccgc gagaagggcg gagaggggga gggccagcca
     7201 gaaagacagg ccgcctttgg tcagaagagc taccccataa gccccgatgc cgaaaaagcc
     7261 ggcgtgagcc agggaggcct ggccggtgta ccccaggatc aggttcatgc tgagcgtggc
     7321 gattgagaaa aggcagccca tgat
//
LOCUS       DASPRR010000213        15745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1498574, whole genome shotgun sequence.
ACCESSION   DASPRR010000213 DASPRR010000000
VERSION     DASPRR010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15745)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15745
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498574"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /gene="pilQ"
                     /locus_tag="VEL68_08200"
     CDS             <1..1648
                     /gene="pilQ"
                     /locus_tag="VEL68_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HXZ35985.1"
                     /translation="PMAEKTPPAEPPKTEQVAEAPSKAKEDVAPAAPVPEKPAIKEEK
                     VPPPESPKPVTVAEALPKAKEEAAPPPPAPEKPSVNEEKALNAETKPPEPEKAPVGAY
                     KGSKISLDFKDADIHNILRLIAEVSNLNIITSDDVKGKITIRLVNVPWDQALEVILAT
                     KNLMKTEEGNVLRITTAETIRKEMEDKQKEQSNLIKTMENRENMEELFTETFLLNFAK
                     ASDIQKLLSGAGTGAGVGGLKPILTPRGSARADDRTNTLIVRDIRSRIEEVESLVKKL
                     DMPTRQVLIEARVVQATSTFARAIGVQWGGSFNQTGTSAWGLTGNNPSAAAGWGFTPN
                     QAGPSASTQLIMPSNFVVNFPASTTNTPAGGMGISFGKLTGNLVNLDFRLQLGETDGY
                     TKVIARPKLATLDNQEAHIKQGEKIPYETTSQAGTQVQFIDAVLQLKVKPHVTPDGSI
                     LMGVKVNRDARGSFRSPVNQVPSIDTREAETEVLVNDGETLVIGGIYETENDQTTEGI
                     PWLMKVPILGWLFKNQETLITKKELLIFLTPSVIQKKREI"
     gene            complement(1913..2227)
                     /locus_tag="VEL68_08205"
     CDS             complement(1913..2227)
                     /locus_tag="VEL68_08205"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ35986.1"
                     /translation="MLVLLLRTGMEPHVAAFWPNLFVIVTGLIIANGRMRIREVLDCL
                     EWGTKNALAIGCACAAVGFIVGTTTLTGLGLKFASATVNLASGTSAWLQTIALLRFFA
                     LN"
     gene            2739..4373
                     /locus_tag="VEL68_08210"
     CDS             2739..4373
                     /locus_tag="VEL68_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ35987.1"
                     /translation="MKAIKLAFITMLAVVVLFGLGAPWALAQTLRIALDAGDIADLNP
                     HIGHSTQDRGILHLIFTGLVRYSPGDIGKFEPDLAEKWDVSKDGKVWTFTLRKGVMVH
                     PWKNNPGYELTSEDVVYSFKSAADPKRSAWAGEYAGMTFEAADKYTVKLTLQKPLSSI
                     LLLPKVADYAGGLIIPKKPAEELGEGFRTQPVGTGPFIFSKYLPKEKVVTVRNEKFYR
                     GVPKLAGVEVFFMPDLSTRKFALQSKEIDVIEGPPSQPWVEEMQVLPGVKVNIFGPGE
                     AGVLHFNATKKPFDDIRVRRALAYALSRDEVVTTIGKAIAAPVYSEIPPPQAGWMSRE
                     ELLQRGQKDKKDYLYEFDRNKAKKLLAEAGFPKGFTTEISMTERGEYQVPIQNIVAQL
                     REAGIDVKLKVVDHPTYHSNIRKDMNTIVLFNCWRPTTDVRLTHFYHSDSIVVTGKKP
                     ITNFSHVGMIDVDGDKKVDNLDSMIESAREMLDAEKQVARWKEVQLKVIDWCLSYPVF
                     IKKFTFARQEYVDWGYDLKSTLILSPQINELTKINK"
     gene            4451..5392
                     /locus_tag="VEL68_08215"
     CDS             4451..5392
                     /locus_tag="VEL68_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35988.1"
                     /translation="MLAYSLRRVLIVVPTLFVVLTITFVLIRVAPGDPAEVRLGDYAS
                     KEAVEALRAEMGLNRPIWLQYLEAMKGLLLGDFGRSMVNGRPIAADLVRSLPYTLELT
                     LAGIVFGALLGIPTGVYTALKRNTVADYLGRTLSLGGLSFPTFYLGILLMLVFAIKIP
                     IFPVVGGGPLDDLPKNLYHLFLPGLTLGLIMTAYVTRMARSSVLNILNEDYVRTARAK
                     GLAERKVIYKHLLRNALLPITALIGVYAIVMIGGSVMVEIVFSRPGLGRMMIGALKQR
                     DYTTLQAVMVTFTVFVVFINLLTDLMYGLIDPRVRYK"
     gene            5404..6267
                     /locus_tag="VEL68_08220"
     CDS             5404..6267
                     /locus_tag="VEL68_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ35989.1"
                     /translation="MEKGIKPTGARGKILKIFFRNRTSVVGAGISLIMLFIAVFAPWI
                     SPYDPLKQNVYHRLTPPEHSNLLGTDEYGRDVLSRVFTGARISFLISLVSVAFGMVIG
                     TLVGVIAGYAGGKVESALMRLLDIIMSFPDEVFGVMVMIVLGSGMENVIVAITFLMIP
                     RFARMAHAPTLAIKEVDYIASARAIGASRYRIVLGHILPNIFGEILVMSTLWLGTAIR
                     LEASLSFLGVGVPPPTPTLGNMVRMGVDFLAVAPWISVFAGLAILVAIFGFNLLGDGL
                     RDITDPKLYAR"
     gene            complement(6527..7303)
                     /locus_tag="VEL68_08225"
     CDS             complement(6527..7303)
                     /locus_tag="VEL68_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXZ35990.1"
                     /translation="MMEDSARSQEALASGKAMIEINDLHKWFGEFHVLQGIDLSVNRQ
                     ERIVICGPSGSGKSTLIRCINRLEEHQRGRIVVDGIELINNIKNIEKIRAEVGMVFQH
                     FNLFPHLTILENLTLGPIWVRKVPKKQAEETAMYYLEKVHIAEQARKYPGQLSGGQQQ
                     RVAIARSLCMNPKVMLFDEPTSALDPEMIKEVLDVMIDLAREGMTMIVVTHEMGFARS
                     VAHRVLFMDFGKIVEGNSPDKFFDNPQYERTKLFLSQILR"
     gene            complement(7550..8644)
                     /locus_tag="VEL68_08230"
     CDS             complement(7550..8644)
                     /locus_tag="VEL68_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXZ35991.1"
                     /translation="MKQAAQGMEAETLKPPITSVGPIGWVKNNLFNGIFSSILTVVTL
                     FFLWKVVPPIFRWAFLDSAWYAPSQACRQIAGACWSVIANNYRFILFGFYPYDQQWRP
                     LVAMLILFGLLFYSRDRGHWKKPLAYAWVLGLGAMGLLMAGGISGLPPVESTQWGGLP
                     LTLLLAVFGLTAAYPLGVVLALGRRSKMRGVKILCIAYIELIRGVPLISLLFMSSIIF
                     PLFLPEGVTFNKILRAQAAMILFTAAYIAEVVRGGLQAMPRGQYEAADSLGLNYYLTM
                     RLVILPQALKIVIPPSVSILISAFKDTSLVVIIALFDLLKTTQTVLSKPEWMGFSREA
                     YIFVAILYFLGCFSMSNYSRKLEKELSAGR"
     gene            complement(8653..9837)
                     /locus_tag="VEL68_08235"
     CDS             complement(8653..9837)
                     /locus_tag="VEL68_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXZ35992.1"
                     /translation="MSDIQDGVAPEKVPFWLDPKKRNLLFQFITFCLVGLLAYYLVSN
                     TLVNLQKQKIASGFGFLEKEASFEIGESLIPYSAASSYARALLVGALNTIKVSFIGIV
                     LTILLGTVIGVARLSSNWLVSKLAAIYIEVMQDIPVLLQLFFWYAIFYETLPPPREAI
                     YPGAGIYLCNRGVAFTVPEAHSAHQYMLLAFLIGGAAIYGIRRWAKKRQEETGKLFPT
                     LLVGLSLLLGLPGVTWLAFGAPLKMDVVRLEGFNLTGGVMLSPEFIALLMGLILYTAA
                     YVAEVVRAGIQSVSKGQKEAAMAIGLRPGLVLNLVILPQALRVIIPPLTSQMLNLTKN
                     SSLAVAIGYPDFVSVANTTMNQTGQSIEGVALIMIAYLFFSLSTSAFMNWYNKRVALV
                     ER"
     gene            complement(9904..10923)
                     /locus_tag="VEL68_08240"
     CDS             complement(9904..10923)
                     /locus_tag="VEL68_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ35993.1"
                     /translation="MKGVKCFAAVLVLLALASSGMAVAGTLDEVRARGMVVVGVNVGV
                     AGFSMPDEKGVWKGLDVDTAKAIAAAVFGDTNKVKYVPLTSVQRLPALQSKEVDVLCR
                     NTTATLTRETVNGLNFVHPNYYDGQGFLISKKMGVKNAKQLKGATVCTLPGTTTEMNA
                     ADFFRKNGMQWKPVVIDSNPELNKALFAGRCDCLTSDASQLAAIRSVAPNPDDYIILP
                     EIISKEPLAPAVRHGDDQWYDIVNWTVMALIEAEELGVTSKNVDEMLQSKDPQIQRFL
                     GVTPGMGKALGLDEKWAYNIIKQVGNYGEIFDRNVGLKTPLRLERGLNDLWTRGGLMY
                     AIPFR"
     gene            complement(11219..11911)
                     /locus_tag="VEL68_08245"
     CDS             complement(11219..11911)
                     /locus_tag="VEL68_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXZ35994.1"
                     /translation="MNFDWGAIVANAKFLLVQGLLGFGIYGGGTLRLAIPAILLGFVI
                     GIILGLARMASRPWVHYPAVVYIEFIRGVPLVIVIFYFWFLVPVLIGRPVPEYWVALT
                     AFVVFEGAYIGEIIRAGIQSVPKGQVDAATASGLNYTQTMIHVILPQAVRNMIPSLVT
                     QFIVLFKDTSLASIIGMMDLTKAAQTVSEREIKPFEMYLFIAVVYWCFTYSMSRFAQW
                     LENRMSPDRPRY"
     gene            complement(11908..12633)
                     /locus_tag="VEL68_08250"
     CDS             complement(11908..12633)
                     /locus_tag="VEL68_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXZ35995.1"
                     /translation="MTYEFKWSVLWTQPYLTWLIDGLLTTLRLASISWCIAIFLGIIF
                     GAMRTVPFRPVRTIATFYVEFFRNVPLLVWLFFWYFGAPEAFPEVIRQWFIRHGIEFW
                     SAVAAISVYHGARFAEVIRAGIQAIPRTQLEAGLSSGLSVVATYRLIIIPIALRLIVP
                     PATNESLNLLKNTSLAMTIGVAELTFAARQVETYSAKAVEALTSAALIYLVFCLSISS
                     VMNWIEAKFAIPGLIARGARAEH"
     gene            complement(12707..13567)
                     /locus_tag="VEL68_08255"
     CDS             complement(12707..13567)
                     /locus_tag="VEL68_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXZ35996.1"
                     /translation="MKRFGFIFLLALLVAALAGTSFAQVQPTLEKIKTSGVFTIGVRN
                     GSPPFSFINKQNERAGFSNDLAELVHKAIEKKLEKQIKLEMKETSPTTRIPLLTSMAV
                     DLVTETMTDTRARRDSVDFSLTFFVTGADFIVKKGSPIKGTKDIAGKRIGTQQGSTNE
                     RVLREKYTQAQLVSFPDQAAAFMALQQGKLDAYCNDAIQLWGLKYKSATPDQWVVVGD
                     WISYEPYGMAMRKNDSDFRAVVNNALMEAIEGGEYFKLYDKWFGTKGEVPYPMSQAFK
                     SFLLYQVVPK"
     gene            complement(13608..14336)
                     /locus_tag="VEL68_08260"
     CDS             complement(13608..14336)
                     /locus_tag="VEL68_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXZ35997.1"
                     /translation="MIEFKAVHKWFGPLHVLNDINLFIKPGEVVVVCGPSGSGKSTLI
                     RCINRLEPIQKGTISVDGMSLQDRKTNLTKLRAEVGMVFQQFNLYPHKTALQNIVLAP
                     VKVRKQTREEAERIAKDLMAKVGIPDKLGSYPANLSGGQQQRVAIARALAMQPKIMLF
                     DEPTSALDPEMINEVLEVMTNLAREGMTMIVVTHEMGFARRVSHRIIFMDEGRIIEEG
                     SPDTFFSQPKSERTRAFLSTILTH"
     gene            complement(14671..>15745)
                     /locus_tag="VEL68_08265"
     CDS             complement(14671..>15745)
                     /locus_tag="VEL68_08265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXZ35998.1"
                     /translation="DFSLGNPNLEPPEEVRKAILETMGDERPGKHAYMPNAGLKETRE
                     AVASFLSKEHGIPMTWEQIIMTCGAGGGLNVALKAILDHGDEVIVLSPYFVEYLFYVD
                     NHNGVGKLAKTSVDFTLDLEEIEAAINPKTKAIVLNSPNNPSGRIYDEISLKKLGKLF
                     EKIKQKNGQSIYILSDEPYSKIVYDGAKVPSVFQAYSESIIATSYSKDLSLPGERIGY
                     LAVNPRMENWKQVIDGLTFCNRILGFVNAPALMQRILVKLQGVAVNVSEYKSKRDNLS
                     RGLSAAGYSFFKPEGAFYLFPKSPIADDVKFVNILLQENILAVPGTGFGTPGHFRLAY
                     CVEDRVIEGSLEGFRRAIHKVSR"
BASE COUNT         3672 a         4261 c         4430 g         3382 t
ORIGIN      
        1 gccgatggcc gagaaaactc cgcccgcaga gcctcctaaa acagagcagg tggcagaagc
       61 tccttccaag gccaaggaag atgtcgctcc tgcggccccg gtaccggaaa aaccggcgat
      121 aaaagaggag aaggttcctc ccccggaatc ccccaaacct gtaacggtgg cagaggctct
      181 tcccaaggcc aaggaggaag ccgctccccc gccccccgcc ccggaaaaac cctcggtcaa
      241 cgaagagaaa gccctgaacg cggagaccaa acctcccgag ccggagaaag ccccggtcgg
      301 ggcctataaa ggaagcaaga tctccctgga tttcaaggat gcggacattc acaacatcct
      361 ccgcctcatc gccgaagtga gcaatctgaa catcatcacc agcgatgatg tgaagggtaa
      421 gatcaccatt cggctggtga acgttccctg ggatcaggcc ctggaagtga tcctggccac
      481 caaaaacctg atgaagaccg aagaagggaa cgtcctgcgg atcaccacgg ccgagacgat
      541 ccgaaaggag atggaggata agcaaaagga acagagtaat ttgatcaaga caatggaaaa
      601 cagggagaac atggaagaat tatttaccga gacctttctc ctgaattttg ccaaagcttc
      661 tgatatacaa aaacttctgt cgggagcagg gaccggagcg ggagtggggg ggttgaagcc
      721 catcctgacc ccccggggat cagcccgggc cgatgaccgt acgaatacgc tgatcgttcg
      781 ggatatccgt tcgcggatcg aggaagtcga gagtcttgtg aagaagcttg acatgcctac
      841 ccgacaggta ctcattgagg cccgcgtggt tcaggccaca agcacctttg cccgagccat
      901 aggggtccag tggggaggca gcttcaacca aaccggcacg tcggcttggg gcctgactgg
      961 taacaaccct tcggccgcag caggatgggg cttcaccccg aaccaggccg ggccaagcgc
     1021 aagcacccaa ctcatcatgc cttcgaactt cgtggtgaat ttcccggcct ccaccaccaa
     1081 cactccggcg ggtggaatgg gaatttcctt tggaaaatta accgggaatc tggtgaacct
     1141 ggattttcgt ctccagttgg gcgagaccga tggctacact aaggtcattg cccggccgaa
     1201 attggcgact ttggataacc aggaagccca tataaagcag ggcgaaaaga tcccctatga
     1261 aaccacctcc caggcaggaa ctcaggtcca attcatcgat gcggtcctgc aattaaaggt
     1321 gaaaccccat gtgaccccag atggaagcat cttgatgggg gttaaagtga accgcgacgc
     1381 ccggggaagc ttccgcagtc cggtgaacca agtgcccagc attgacaccc gcgaggctga
     1441 gaccgaggtc ctggtcaatg atggagagac cctggtcatc ggcggaatct atgaaacgga
     1501 aaacgatcaa accaccgaag gaattccctg gctgatgaaa gtacccattt tgggctggct
     1561 cttcaaaaat caagagacgc tcatcacgaa gaaagagctc ctgatttttt tgactccaag
     1621 cgtcattcag aagaagaggg aaatataaga cccttcgccc tcaatcccca ggggcgccgc
     1681 aaggcgccct tttttttgct gcgccccttg ggcatagtgg ggtaggaaga aggttaggcg
     1741 agctttcccc atcctgattt caaaagctgc ggtgagtctc agagaccaag ctgttctctg
     1801 aattcccttc ccccaactac aaggcagtct gttaattccg agggggggct aggcaggcac
     1861 gctcggcctc ggtttattat ggggcctgtc cctcttgatc ctccgacgtc acctaattca
     1921 gagcgaagaa gcgcagaaga gcgatcgtct gcaaccaggc ggatgtgccg ctggccaggt
     1981 taacagtggc ggaggcaaat ttaagcccca ggccggttaa agtagtagtc cccacgatga
     2041 agcccacggc agcgcatgcg cacccgatgg ccagggcatt cttggtgccc cactccaggc
     2101 agtccagaac ttctcgaatt ctcatccgac cgttggcaat gatcagcccg gtcacgatca
     2161 caaaaagatt cggccagaaa gctgccacgt ggggctccat cccggtccgg agaaggagga
     2221 cgagcatgac cacggcgagt gtccctcatg ggcccttcaa aggggttcca ttgaaagagc
     2281 acgcaggaca ccttggaaca cacggccaca aagaagttct tcgttttggg cccgatttcg
     2341 tttaaggcca cggaactgat gatcccccag aaggcagcga ggaagggggt aaatccgctc
     2401 ataaggaaac agacaatgac aatgaggggc agaaacgatt taagaattga ccggtcctgc
     2461 ggaatagtgt agatttcgtg tgatgacggt cagaaattgg aaagatctac ggagcccagt
     2521 ggtgtcattt cgcgattatg gttccgagat tgaatggaaa ccaaagagtt ttttagccgg
     2581 acggaggttc gggaaagggg tgagaggggg tgagaaagga agggttttgc ccgaggggta
     2641 tctttcgaat tggatcggaa gatagcgaga agaagggatg gaaatcagcg gataagcttt
     2701 gctcaagaaa tcttgggaga ggaaaaggag aaattaagat gaaagcgata aaattagcat
     2761 tcataactat gcttgcggtc gtcgtgttat ttgggcttgg cgcgccctgg gccctggctc
     2821 aaacccttcg gatcgccttg gatgcgggag atatcgccga tctcaacccg cacatcggtc
     2881 atagtaccca ggacaggggc atcctgcatt tgatcttcac cggcctggtc cgctactctc
     2941 ccggggacat cggcaaattt gagccggacc tggccgagaa gtgggacgtt tccaaggacg
     3001 gcaaggtctg gacttttacc ctgcggaaag gcgtgatggt ccatccctgg aagaacaacc
     3061 ccgggtatga attgacctcc gaggacgtgg tctattcgtt caagtcggcg gcggacccca
     3121 agcgatcggc ctgggcggga gagtatgcgg gaatgacctt cgaagcggcg gacaagtaca
     3181 cggtgaagct gactttgcag aaaccgctgt cttctatctt gctccttccc aaggtggcgg
     3241 attacgccgg cggcctcatc atccccaaga agcccgcaga agagctgggg gaaggcttcc
     3301 ggactcagcc cgtgggaacg ggcccgttca ttttcagcaa gtacctccct aaggagaaag
     3361 tcgtcaccgt gcgcaacgaa aaattctacc gcggcgtgcc gaagctggcc ggggtggagg
     3421 ttttcttcat gcccgacctg agcacccgga agtttgccct gcagagcaag gagatcgacg
     3481 tcatcgaagg cccgccttcg caaccctggg tggaggagat gcaggtcctg ccgggggtca
     3541 aggtgaatat cttcggcccg ggagaagccg gcgtgctcca tttcaacgcg actaagaagc
     3601 ccttcgacga catccgggtg cgacgcgcgc tcgcctatgc actctcgcgt gatgaggtcg
     3661 tgacgaccat cggtaaagcc atcgcggctc cggtgtattc ggaaattccc ccccctcagg
     3721 cgggatggat gtcgcgggag gaactcctgc agcgcggcca gaaggataaa aaggactacc
     3781 tctatgaatt cgaccggaat aaggccaaga agctcctggc cgaggccggc tttcccaagg
     3841 gattcaccac ggaaatcagc atgacggagc ggggggaata ccaggtgccc atccagaata
     3901 tcgtggccca gctccgggag gccgggattg acgtcaagct gaaagtggtc gatcatccga
     3961 cctaccacag caacatccgg aaggacatga acaccatcgt tctcttcaac tgctggaggc
     4021 cgacgacgga cgtgcgcctg acccacttct atcattccga ttccatcgtt gtgaccggca
     4081 agaaacccat taccaatttt tcccatgtgg gaatgatcga tgtggacggc gacaagaagg
     4141 tggacaattt agattccatg atcgagtccg cccgggagat gcttgacgcc gagaaacagg
     4201 tggcacggtg gaaggaagtt cagctgaaag tcatcgactg gtgcctctct taccccgtgt
     4261 tcatcaagaa attcactttt gcgcggcagg agtatgtgga ttggggttat gacctgaagt
     4321 ctaccctgat cctctccccg cagatcaacg agttgaccaa gataaacaag taagcagccc
     4381 ttgccggggg ggcaggggta tgccggcgca tattcctgcc tccggccgga tgataccgga
     4441 gtaaggttcc atgttggctt acagcctccg gcgggtcctg atcgtcgtac ccactctttt
     4501 cgttgtactg acgatcacct tcgtgctcat acgagttgcc cccggcgacc ctgcagaagt
     4561 gaggcttggg gattacgcct ccaaggaggc cgtcgaggct ctgcgcgccg agatggggct
     4621 caaccggccc atctggcttc agtatcttga ggccatgaaa ggcctgctcc tgggggattt
     4681 tgggaggtcc atggtgaacg ggcggcccat cgccgcagac ctggtccgaa gccttcccta
     4741 caccctggaa ctcaccctcg cgggaatcgt cttcggggcg ctgctgggaa tccccacggg
     4801 cgtgtatacc gccctcaagc ggaataccgt cgcggattac ctggggagaa ctctgtctct
     4861 cggcggtctg tcgtttccga ctttttacct ggggatcctg ctgatgctgg tattcgccat
     4921 aaagatcccc atcttcccgg tggtaggcgg cggacccctg gatgacctgc ccaagaatct
     4981 ctaccaccta tttcttcccg gtctcaccct gggattgatc atgacggcct atgtgacccg
     5041 gatggcgcgc tcctcggtgc tcaacatcct gaatgaagat tacgtgagaa cggcaagggc
     5101 caagggcctg gccgagcgga aggttatcta taagcacctg ttgcgcaacg cgttgctccc
     5161 cattaccgcc ctgatcgggg tctatgccat cgtgatgatc ggcgggtcag tcatggttga
     5221 gatcgtcttc tcccgtccgg gcttgggcag aatgatgatc ggtgccctga agcagcgcga
     5281 ttacacgacc ctccaggcgg tgatggtcac cttcacggtc tttgtcgtat tcatcaacct
     5341 cctgacggac cttatgtacg gactcatcga cccacgggta agatataaat agggtttcgg
     5401 aaaatggaaa agggaattaa accaactggc gcgcggggaa agatcctgaa gatttttttc
     5461 cggaatcgta cctccgtcgt cggcgccggc atttctttga tcatgctctt catcgccgtc
     5521 ttcgctccgt ggatttcccc ctatgatccc ctgaagcaga acgtctatca tcgcctgact
     5581 cccccggaac atagcaacct tctgggaacg gatgaatacg ggagggatgt cctctcccgg
     5641 gtcttcacgg gtgcgcgaat ctcgttccta atcagcctgg tctccgtcgc cttcggcatg
     5701 gtcatcggca ccctagtggg agtgatcgca ggatacgccg ggggaaaggt ggaatcggcc
     5761 ctcatgcgtc tgctcgatat tatcatgtcg ttccccgatg aagtattcgg ggtcatggtc
     5821 atgatcgtgc tgggctcggg gatggaaaac gttatcgtgg cgatcacctt cctgatgatt
     5881 ccgaggttcg cccggatggc ccatgcgccc accctggcaa tcaaagaggt ggattatatc
     5941 gcctcggcca gggcgatcgg cgcaagccgc taccgcatcg tcctggggca tattctcccc
     6001 aacattttcg gggagattct ggtgatgagc accctctggc tgggtacggc gatccggctg
     6061 gaggctagct taagcttcct gggcgtggga gttccccctc cgacccccac cctgggcaac
     6121 atggtaagga tgggggtgga cttcctggca gtcgccccgt ggatttcggt tttcgccggg
     6181 ctggccattc tggtggcgat cttcggtttc aatctgctgg gcgacgggct gcgggatatc
     6241 actgacccca aattgtacgc gcgctgaggt gagaaacggg cggggagaat ttgttcgcag
     6301 cagggcgggg gaggccgcta ttccgtatcc acttttttca gcacggcgcc ggaacaccag
     6361 attctcgtaa tcaggggaag ccaaagaaag aagaaggggc atagatgaac gcgggtggac
     6421 gcggataaat attcgaaaaa tttcgcccac ccccaactct cggtcgtcgt caacggactc
     6481 ggtccggaga gcgggcccag ttctttatcg atgggtgtca agactttcag cggaggatct
     6541 ggctcaggaa gagtttggtc cgttcgtact gagggttgtc gaaaaactta tcggggctgt
     6601 ttccctcgac gatcttgccg aagtccataa aaagcaccct gtgggccacg ctcctggcaa
     6661 agcccatttc gtgggtgacg acaatcatgg tcatcccttc ccttgcaaga tcgatcatga
     6721 catccaacac ctccttgatc atctcgggat cgagcgcgga ggtgggttca tcgaaaagca
     6781 tcaccttggg attcatgcac aggctccggg caatggcgac ccgctgctgc tggcctccgg
     6841 aaagttgtcc cggatacttc cgggcctgct cggcgatatg gaccttttca aggtaataca
     6901 tcgccgtctc ttcggcctgt ttcttcggga cctttctcac ccagatgggc ccaagagtca
     6961 gattctccag aatcgtgagg tgaggaaaca ggttgaagtg ctggaagacc attcccactt
     7021 cggctcggat cttctcaatg ttcttgatgt tgttgataag ctctatccca tctacgacga
     7081 ttctgccccg ctggtgctcc tccaatcgat tgatacagcg gatcagggtc gattttcccg
     7141 aacctgaagg gccgcagatg acaatccgct cctgcctgtt gaccgacaga tcgatgccct
     7201 gaagcacgtg aaactcgcca aaccacttgt gcaaatcgtt gatctcgatc atcgccttac
     7261 cggaggccag cgcttcttgg ctcctggctg aatcttccat cataaatacc tcatgacggt
     7321 tttggggact cgtgattgga atcttaacgg gaaatacaat cgaaagcgtt catgatgagt
     7381 cccctcgaac catggaaccc gctctcgaca agcatgtcct gaatgccctt cgatcccgcg
     7441 cttcgcggga cttaggccga atggatcagg acccattatg gtgagcccgt tcaatctttt
     7501 ttcctccatt ttgcatcttt ccatctgtac ttgccatttt catcgatgcc tatcttcctg
     7561 cggaaagctc cttttccagc ttccggctgt agttggacat ggaaaaacag cccaggaagt
     7621 agagaattgc gacgaagata taggcctccc ggctgaagcc catccactcc ggtttggaga
     7681 gaacggtctg cgtggtcttg aggaggtcaa agagcgcaat gatcaccacg agggaggtat
     7741 ctttgaacgc tgaaatgaga atgctgaccg agggcgggat cacgatcttc agcgcctgcg
     7801 gcagaatgac cagcctcatc gtgaggtaat aattcagccc cagggagtcc gccgcctcat
     7861 actgtcctcg gggcatcgcc tgaagccctc ccctgaccac ttccgcaata taggcggcag
     7921 tgaaaagaat catggccgcc tgcgcccgga ggatcttgtt gaaggtaacc ccttccggca
     7981 gaaagagggg gaagatgatg gatgacatga atagaaggct gatcaggggc acgccgcgga
     8041 tcagttcgat ataagcgatg cagagaatct tcactccgcg catcttggac cgccgcccca
     8101 acgccagaac gacgccgaga ggataggcgg cggtaaggcc gaagacggca agaaggagcg
     8161 tgagcggcag tccgccccac tgggtgctct cgacgggagg gagtccggag atccctcccg
     8221 ccatgagcag acccatggcg ccaaggccca atacccaggc gtaggccaac ggcttcttcc
     8281 agtggccccg gtctcgactg tagaagagaa ggccgaagag gatcagcatg gcgacaaggg
     8341 gacgccactg ctggtcgtag ggatagaacc cgaacagaat gaagcggtag ttgttggcga
     8401 tgacggacca gcacgcgccg gcgatctgtc gacatgcctg gctgggggca taccatgcgc
     8461 tgtcaaggaa tgcccagcgg aagatgggag gaacaacctt ccacaggaaa aagagggtga
     8521 cgaccgtcag aatggagcta aagatcccgt tgaagaggtt gttcttcacc cacccgatgg
     8581 gaccgacgct ggtgatgggg ggcttcaggg tttctgcttc cattccttga gcagcttgtt
     8641 tcatggaggc ccctatcttt caaccagcgc cactcgcttg ttataccagt tcataaaagc
     8701 cgaagtggag agactgaaaa agagataggc gatcataatc aaggcaaccc cctcgatcga
     8761 ctgccccgtc tggttcatcg tcgtattggc caccgaaacg aagtccggat atccaatggc
     8821 taccgccagg gagctgtttt ttgtcaagtt gagcatctgg ctggtcaggg gcgggatgat
     8881 gacccggagc gcctgaggca ggatgaccag gttgagaaca aggccgggcc tgaggccaat
     8941 ggccatagcc gcttccttct gccctttgct taccgactga attcccgcgc ggaccacctc
     9001 cgccacatag gccgcggtat aaaggatcag gcccataaga agcgctataa attcgggact
     9061 gagcattacg ccgcccgtca aattgaaccc ttcgagtctc acgacatcca tctttagcgg
     9121 agcgccaaag gccagccagg taacgccggg aaggccgaga agaaggctga ggccaaccaa
     9181 caaggttggg aaaagcttcc ccgtttcctc ctgacgtttc ttcgcccagc gacgaattcc
     9241 gtatatggcc gctcctccga tgaggaaagc caaaagcata tattggtgag cggaatgggc
     9301 ctcgggaacc gtaaaggcca cgccgcgatt gcacagatag atacctgcgc ccggatagat
     9361 cgcctccctc ggaggaggaa gggtttcata gaagatcgcg taccagaaga acaattgcag
     9421 gagaacgggg atatcttgca tgacctcgat gtagatggcg gccagttttg aaaccagcca
     9481 gttggaggac agcctggcta ccccgatcac ggtcccgaga aggatggtga ggacgatccc
     9541 gataaatgag accttgatgg tattgagggc gccgaccaga agggcccggg cataggagct
     9601 tgccgctgag tagggaatca gggattcgcc gatttcgaat gaggcttctt tttcgagaaa
     9661 tccaaacccg gacgcaatct tctgtttttg gagattgacc agggtgttgg agacgagata
     9721 ataggccagc agtcccacca gacagaaggt gatgaactgg aaaagaaggt tccttttctt
     9781 ggggtcaagc cagaagggga ccttttccgg cgccacgccg tcctgaatat cgctcatgag
     9841 taaatacagg tacccgtgag gcgctccccc ctcgaagagc gcctcacctc caagcttaaa
     9901 agatcatcgg aatgggatgg catacatcaa gccgcccctt gtccacaagt cgttgagccc
     9961 tcgctccagt ctgagcgggg ttttgaggcc cacgttgcgg tcgaatattt caccgtaatt
    10021 gcccacttgt ttgataatgt tataggccca tttttcatca agaccgagcg ctttgcccat
    10081 gccgggagtg acccccagga aacgctggat ttgaggatcc ttgctttgga gcatctcatc
    10141 cacgtttttt gaggtaaccc ccagttcttc cgcctcaatg agagccatca ccgtccagtt
    10201 gacgatgtcg taccactgat cgtccccatg acggactgcg ggcgcaagag gttctttcga
    10261 aatgatctca ggcaagatga tgtagtcgtc ggggttcggc gccacggatc ggattgcggc
    10321 caactgagag gcatccgaag tgagacaatc gcagcggccc gcaaacaagg ccttgttcaa
    10381 ttcagggttg ctatcaatga cgacaggttt ccattgcatc ccgttcttac ggaaaaagtc
    10441 cgcggcgttc atctccgtgg tggtcccggg aagggtgcaa accgtggccc ctttcaactg
    10501 tttggcgttc ttgaccccca tcttttttga gatcagaaac ccctgaccgt catagtaatt
    10561 cggatgaacg aaattgaggc cgttcacggt ttcacgggtc aaggtggcgg tggtgttccg
    10621 gcacaggaca tccacttcct tcgactggag cgcggggaga cgctggacgg aggtgagggg
    10681 aacatattta accttattcg tatcaccaaa aacggctgcc gcgatcgctt tcgccgtgtc
    10741 cacgtcgagg cctttccata cgcccttctc gtcaggcatg ctgaatccag ccactccgac
    10801 attgacgccg accacaacca ttccccttgc ccggacttcg tcaagggttc ctgcaacggc
    10861 catgccgctt gaggcaaggg ccagaagaac cagaacggcg gcaaagcatt tcactccttt
    10921 cattttacct acctcctttc aaatttgatc atgggcgcca attcagctgt ttgaaatcgc
    10981 tttataaaat ccctcgcggc ctcccttggt taaaggggag gagatttatc ccttccggtg
    11041 acttgaagtc tacctttagg aaacggaacg tgtcaataaa aatgagacaa aatgagacaa
    11101 atgatccgcc cagacccatt ggagcggcga tagaaccagc agcggggagg caccggaaag
    11161 aggggaaaag ccctccgttt accgggaact gaaggggcgg aatctcgtca tgccaatttc
    11221 aatagcgggg acgatccgga gacatgcggt tttccagcca ctgcgcgaaa cgggacatgc
    11281 tgtaggtaaa gcaccagtac actacggcga tgaagaggta catctcaaaa ggcttgattt
    11341 ctctttcgct caccgtttgg gccgctttgg tcaagtccat catgccgatg atcgatgcca
    11401 gggaggtgtc cttgaaaagg acaatgaatt gcgtgaccag cgagggaatc atgttacgga
    11461 ccgcctgggg cagaatcaca tggatcatgg tttgggtgta gttgagcccg gaggcagtgg
    11521 ccgcgtcgac ttgccctttc ggcaccgact ggattccggc ccggatgatt tccccgatgt
    11581 aggcgccttc aaagaccacg aaggcggtca gcgccaccca gtactcgggg acggggcggc
    11641 cgattaggac tggaaccagg aaccagaaat agaaaataac gataaccaag gggacgccgc
    11701 ggatgaattc gatgtagacc acggctgggt aatggaccca gggacgcgag gccatgcggg
    11761 ccaggccgag gatgatgccg atcacgaacc cgagcaggat ggccggaata gccagccgga
    11821 gcgttcctcc cccgtaaatc ccgaacccta gaaggccctg gaccaggagg aatttggcat
    11881 tggcgacgat ggccccccag tcgaagttca atgttcggcc ctcgctccgc gggcgatgag
    11941 cccggggatg gcgaattttg cttcgatcca gttcatgacg ctggaaatcg acaggcagaa
    12001 gaccaggtag attaaggccg ccgaggtcag cgcttctacg gccttagccg agtacgtctc
    12061 gacctggcgg gccgcgaaag tgagttcggc caccccgatc gtcatagcca aagaggtgtt
    12121 ctttaggaga ttcaacgatt cgttggttgc cgggggcacg atcaggcgca gggcgatggg
    12181 gatgatgatc agccggtatg tggcgaccac cgagagcccc gaagaaagcc cggcttcgag
    12241 ctgggtccgg gggatggcct ggatacctgc ccggattacc tccgcaaaac gggccccgtg
    12301 gtaaacgcta atcgccgcca ccgcggacca gaactcgatc ccgtggcgta taaaccactg
    12361 gcgaatcacc tcgggaaacg cctccggggc tccgaaatac cagaaaaaaa gccataccag
    12421 aagcggaacg ttccggaaaa actccacgta gaaggtcgcg attgtccgga ccggacggaa
    12481 ggggaccgtg cgcatggccc cgaagataat ccccaagaaa atcgcaatgc accaactgat
    12541 tgaggccagc cgaagggtgg tcaggagtcc atcgatgagc caggtaaggt acggctgggt
    12601 ccacagaacg gaccacttga attcataggt catgcctaaa aagccaaaaa acatctaaga
    12661 cccaaatccg gggaggggtc gcctccccgg aatctttcct tggtggctat ttgggaacta
    12721 cctgatagag gaggaaggac ttgaaggcct ggctcatggg atagggaact tcgcccttgg
    12781 ttccaaacca cttatcatag agcttgaaat actctccgcc ctcgatggct tccatcaggg
    12841 cgttgttgac cacggcgcga aaatcggagt cattcttgcg catggccatc ccgtagggct
    12901 catagctgat ccagtctccc acgacaaccc actgatcagg agtggcggat ttgtacttca
    12961 acccccagag ctgaatggcg tcattacagt aggcatccaa cttgccctgc tgaagtgcca
    13021 tgaacgcggc ggcctggtcc gggaagctta ccaactgggc ttgagtgtac ttttccctca
    13081 ggacccgctc attggtcgag ccctgttggg tgccgattcg tttcccggcg atatccttgg
    13141 tccccttaat cgggctccct ttttttacga tgaagtcggc gccggttacg aagaaggtca
    13201 agctgaaatc cacgctgtca cgccgggccc gggtatcagt catggtttcc gttaccaagt
    13261 ccacagccat gctcgtcaga aggggaatcc gggtggtcgg ggaagtttcc ttcatctcca
    13321 acttgatctg tttttccagt ttcttctcta tggccttgtg aaccaactcc gccaggtcat
    13381 tcgagaagcc ggctctttca ttctgtttgt tgatgaatga gaaggggggg gacccgttgc
    13441 gcaccccaat cgtaaagacc cccgaggtct tgatcttctc aagagtaggc tggacctggg
    13501 cgaaggaagt tcccgcgagg gccgccacta gaagggccaa aagaaaaata aatccgaatc
    13561 ttttcatctt gctttgcctc cttttcggga tttttatgac gtggctctta gtgcgtcaga
    13621 atggtactca agaaagctct cgttctttcg ctcttgggct gggagaagaa agtatccggg
    13681 gagccctctt caatgatccg accttcatcc atgaagatga tgcggtggga tacccgccgg
    13741 gcaaacccca tttcgtgggt gaccacgatc atggtcatgc cttcccgggc caggttggtc
    13801 atcacctcga ggacttcgtt gatcatctcc gggtccaggg cggaagtggg ttcatcgaag
    13861 agcatgattt tgggctgcat ggccagcgct cgcgcgatgg ccacccgctg ctgctgtcca
    13921 cccgagaggt tggccggata gctcccgagc ttatcgggga tccccacctt ggccatcagg
    13981 tccttggcaa tcctctcggc ctcctcccgg gtctgtttgc gcacctttac cggggccagg
    14041 acaatatttt gcagggcggt tttgtggggg tagagattga actgctggaa aaccatcccc
    14101 acttcggccc taagcttggt aaggttggtc ttgcggtcct ggagggacat cccgtcaacg
    14161 ctgatggtgc ccttttggat gggctccaac cggttgatgc agcgaatcag ggtactcttg
    14221 ccggaaccgc tgggcccgca gaccacgaca acctctcccg gcttgataaa aaggtttata
    14281 tcattcaata catgaagggg cccgaaccat ttatggaccg ctttgaattc aatcatgctg
    14341 cgcttccctt tgccggcaat aaatctttta aagcctaaca aatgggggag gagagtgtca
    14401 atagcaaatt cccttttggg tacaccgttc ccagcggaat ttgcggcatt tgacttatct
    14461 tccttaatgg ttcaagaaaa aataatattc ggtggctatg cggagcagag tcgtctgaca
    14521 acctatcatt tcagcggttg tttggcggcc agattttaac tttgccttta gtttacataa
    14581 tatatattat cagacgttat ggagtattaa agtaacttct caaggaaggc agaagtcaac
    14641 ctcttaatgc ttgccagctc gcgagaatgt ttagcgagag accttgtgga tggcccggcg
    14701 aaaaccttcc agggagccct caataaccct gtcctctacg cagtaagcga ggcggaaatg
    14761 ccctggagtc ccgaagccgg ttcctggcac agcgagaatg ttctcctgga gaagaatatt
    14821 cacaaactta acgtcgtcag ctatggggga ttttgggaag agatagaaag ccccttccgg
    14881 cttaaaaaat gaatacccgg ctgcggacaa tccccgactt aggttatccc gcttcgactt
    14941 gtactccgat acattgacgg cgactccctg aagcttgacg agtattctct gcatcagcgc
    15001 gggcgcattg acgaacccca gaatccggtt gcaaaacgtg aggccgtcaa tgacttgctt
    15061 ccaattttcc atcctgggat tgacagctag ataaccgatc cgctctccag gcaaagagag
    15121 gtccttggaa tatgaggttg cgatgatgct ttctgaataa gcctgaaaga cactgggaac
    15181 cttggcccca tcgtaaacga ttttgctgta tggttcgtcg gacaggatat agatactttg
    15241 accatttttt tgtttaattt tttcaaatag tttccccaat ttttttaaac ttatctcatc
    15301 ataaatgcgg cccgatgggt tattgggcga gttcaagacg attgctttag tcttgggatt
    15361 gatagctgcc tcaatttctt ctaagtctaa tgtaaaatct acacttgttt tagctaactt
    15421 accgacacca ttgtgattat caacatagaa tagatactcg acaaaatagg gggaaagaac
    15481 tatcacttcg tccccatggt cgaggatggc tttcagggca acatttaagc ccccgccagc
    15541 cccgcaagtc atgataatct gctcccaggt catgggaatc ccatgttctt tggagaggaa
    15601 cgaagccaca gcctcgcggg tttccttcag ccctgcattg ggcatataag cgtgctttcc
    15661 ggggcgttca tcccccatag tctcaagaat ggccttccgc acttcctccg ggggctccag
    15721 attcgggttt cccaggctaa aatca
//
LOCUS       DASPRR010000214         5633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_538296, whole genome shotgun sequence.
ACCESSION   DASPRR010000214 DASPRR010000000
VERSION     DASPRR010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538296"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VEL68_08270"
     CDS             complement(<1..729)
                     /locus_tag="VEL68_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ35999.1"
                     /translation="MIADSRLRIADLIITNGTLLPMASGSDPVQNGAIAISDGRIAAM
                     GANSEIEKTFDAGTTIDARGGLIMPGLVNSHTHAAMTCYRGMADDLPLMEWLNYYIFP
                     AEAKSDGDQVHWSTLLACAEMIRSGTTTFCDMYLFEDQVAQAAKQAGMRAVVGEALYD
                     FPSPHYGPIEQGLEFAESLIQYWQNDTLIRVAVEPHALYTCSPDLLKRCRQMADRYGV
                     PLIIHLSETQSEIEEVYKKYGKRPV"
     gene            complement(726..1547)
                     /locus_tag="VEL68_08275"
     CDS             complement(726..1547)
                     /locus_tag="VEL68_08275"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXZ36000.1"
                     /translation="MNQTLKEIEEAYRSISRRTRMKPRVGIILGTGLGKLVDRVSIRE
                     SLSYDDIPHFPSSTVEGHAGRLLFGRLAGKNVVIMQGRAHYYEGYSLSKITLPVRVMK
                     RLGIEILIISNAAGGLNPLFSAGDVMVISDHINLTGQNPLIGPNLDLFGPRFPDMVAV
                     YDRELIQIAEQEAAKVRVKMQKGVYVGIIGPSLETPAETRFLRMIGADAVGMSTLPEV
                     IVGVHCGLRILGFSAISNINLPDCMEPATLKEILKNAAVAGRKMIKIVEGVIKRI"
     gene            1987..2283
                     /locus_tag="VEL68_08280"
     CDS             1987..2283
                     /locus_tag="VEL68_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36001.1"
                     /translation="METLQEFVGVFAHKGWLNRAVRIIHRDRRYRIFCSETEFIAHRI
                     NDSCRDSWGFPCWAVCIVTGDQIIEDSDLSGFASIEPGVHEWLRCIAEGDFQIL"
     gene            2297..3223
                     /locus_tag="VEL68_08285"
     CDS             2297..3223
                     /locus_tag="VEL68_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXZ36002.1"
                     /translation="MNIVVSGSLAYDRIMDFPGYFKEHILPEKIHVLNVCFQVNDMKE
                     KFGGTAGNIAYALTLMGQKPVISATVGKDYHRYFDWLKKNGISTEHIRIIEDEFTAGA
                     YITTDRADNQITGFHPGAMKFSSALDFDRLDPRETLMIVSPGNLEDMVKYPQMCKARG
                     IPYIFDPGQSLPMLPAEELVQAIEGCRILIANDYELDMILCKTGLDKEAVLRRAGAII
                     VTLGGLGSRVFTPGCQICIPAAKPKTVKDPTGAGDSYRGGLISGLVQGKGIEQCARMG
                     SVCASFAVECYGTQEYRFSSDEFNERFNGYPG"
     gene            3615..5222
                     /locus_tag="VEL68_08290"
     CDS             3615..5222
                     /locus_tag="VEL68_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXZ36003.1"
                     /translation="MEIVETDVLIIGGGGAGMRAALAAREKGAEVLLVSKTPPGKSTC
                     TYLSGGAFSLAAEGSTVETHFKQTLQAGKGINDPELVKVLVEDAPERVRDLERLGLKG
                     AWGKGRFTCLGKAPSWGAPLTDALADAAREGGVGEKAWVAVFDLLRDGEKVVGALGFE
                     FRKGIPFAFLSKSTVLANGGGGALYPRNDNPVRTTGDGYALAYEAGCRLRDMEFVQFM
                     PIGLAEEGSPPFVLAPSLADAGKVVNSSGLDVLEKYHIVDKPVVVRCRDTFSQAIIKE
                     EERGEKVFLDLRKLSDEAWPKDNMAMSQKRMLRDRFSCSDRLLRIAPTVHFFMGGVCT
                     DSDGRTEVPGLWAAGEVVGGLHGANRMGGNALSEIIVFGSRAGAAAAEWALREKPLGE
                     PGALIRSRWDRFQKKWKSNGKGLSPRVLRQRMGAILWDQGGIIREEKGLQLGLEELKK
                     MKTEDLPWAASETSKEILEKIEVDNGLLVSEMILRSALMRRESRGAHYRKDFAVMDDR
                     NWKGNIFLRKSASGMTLTFHPLPGKSS"
     gene            5498..5574
                     /locus_tag="VEL68_08295"
     tRNA            5498..5574
                     /locus_tag="VEL68_08295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5532..5534,aa:Arg,seq:cct)
BASE COUNT         1301 a         1479 c         1567 g         1286 t
ORIGIN      
        1 tacaggcctt ttcccatatt tcttataaac ttcctcaatt tcactctggg tctccgaaag
       61 atggatgatc aggggaactc cataccgatc ggccatctgc ctgcaccttt tcaaaaggtc
      121 gggggaacag gtgtaaagcg cgtgcggctc cactgcgacc ctgatcaggg tgtcattctg
      181 ccaatattga atcagagatt cagcaaactc gagcccttgc tcaatcggcc cgtagtgggg
      241 ggatgggaaa tcgtacaatg cttcgcccac aacggccctc atccctgcct gcttggccgc
      301 ctgggccacc tggtcctcga aaagatacat gtcgcagaaa gtggtcgtcc cagagcgaat
      361 catctccgcg caggccagga gggtggacca gtgcacctgg tcaccgtcgg attttgcttc
      421 agccgggaaa atgtagtagt tcagccattc catgagggga aggtcatcgg ccatcccccg
      481 gtagcaggtc atagcggcat gggtgtgaga attgaccaag cccggcatga tcagcccacc
      541 tctggcatcg atggttgtac ccgcatcgaa ggttttttct atttccgaat tcgcccccat
      601 cgcggctatt ctgccgtcgg agatggcgat ggccccattc tgaaccggat ccgaccctga
      661 agccatgggc aaaagcgttc cattggtaat gatcaaatcc gcaatccgca atctggaatc
      721 cgcaatcata tcctcttaat gactccttcg acaatcttta tcatcttcct tccagccacg
      781 gcggcatttt tcaaaatttc ttttagcgtg gcaggctcca tgcaatcggg gagattgata
      841 ttggaaatag ccgaaaaccc cagaatccgt agaccgcagt ggactcccac gatcacctcg
      901 ggaagggtgg acattcccac cgcatcggct ccgatcatcc gcaaaaagcg cgtctccgcc
      961 ggagtctcca ggctgggacc gatgattccc acatacaccc ctttttgcat tttgaccctc
     1021 acctttgccg cctcctgctc ggcgatttgg ataagctcgc ggtcatagac cgcgaccatg
     1081 tccggaaaac gggggccaaa aagatcaaga ttggggccga tcaagggatt ctggccggtc
     1141 aggttgatgt ggtcggagat caccattaca tctcccgcgg agaaaagggg attgagacct
     1201 cccgcggcat tggaaatgat caaaatctcg attcccagcc tcttcatcac ccggaccggc
     1261 aaagtgattt tggagagcga atatccctcg taataatgag cccttccctg catgatgacc
     1321 acattcttac cggccaggcg gccaaataag aggcggccgg catgtccttc cacagtgctc
     1381 gaaggaaagt gaggtatatc gtcgtaggac aaggattccc ttatggaaac cctatccacc
     1441 agcttcccca ggccggttcc aaggataatc cccacccgag gtttcatccg ggtccgcctg
     1501 gagattgaac ggtaagcttc ctcgatttct ttcaaggttt ggttcatgaa agaactcctt
     1561 taaagggccg caggttggcc ggaatgttag cacaaaaaag agcccgagtg gcaggataaa
     1621 taatcgttcg gagcgaatga atagctatgg cctttcttcc tttcttcgat gcttggatat
     1681 tccaaattcc tgaaatgcag ctcaccttca ctttcggcag ggaatctaaa gctctgttta
     1741 acttcaggaa taaaatagga gtgaagggtg gctagcctga aatcgcttga cagcaggcga
     1801 cctggttcgg tatttttgtt aataatagga gggagttccg gccatcgtca tttttccgga
     1861 atggagcgtt ccggtccgga gcccataatg ggtgccgatc cggtggattc tttggcaaac
     1921 cccatcgatg ggggtgatgg tatgatggga aacaagattt ttccaggggc tgcggggaaa
     1981 atcccgatgg aaaccctgca agagtttgtc ggcgttttcg ctcacaaggg ctggctcaac
     2041 cgcgccgttc gcatcatcca ccgcgacaga agataccgga ttttctgcag cgagacggaa
     2101 ttcatcgccc atcggatcaa cgatagctgc cgagattcgt ggggcttccc ctgctgggct
     2161 gtgtgcatcg ttaccgggga tcaaattatc gaggattcgg acctgtccgg gttcgcatcg
     2221 atcgaacccg gcgttcatga atggctgcgt tgtatcgccg agggagattt ccaaattcta
     2281 tgagaggccg gtccctatga atatcgtcgt ttccgggtct ctggcctacg atcggatcat
     2341 ggatttcccg ggatacttca aggaacacat cctgcctgag aagatccacg ttttaaacgt
     2401 ctgcttccaa gtgaacgaca tgaaggagaa attcgggggt acggccggca atatcgccta
     2461 cgccctcacc ctgatggggc aaaagcctgt catttccgcc acggtgggca aggattatca
     2521 tcgctacttt gattggctga agaaaaacgg gatctccacc gaacacatca ggatcatcga
     2581 ggatgaattc accgccggcg cctacataac cactgaccga gcggacaacc agatcaccgg
     2641 gtttcatccg ggggccatga aattttcttc cgctctggat ttcgaccgac tcgatccccg
     2701 agaaaccctg atgatcgtct cccccggaaa tctcgaggat atggtgaaat acccgcagat
     2761 gtgcaaggcc agggggattc cttacatctt cgacccgggg caatccctgc ccatgctgcc
     2821 tgcggaagag ctggtccagg ccattgaggg ctgccggatt ttgatcgcca acgattatga
     2881 gctggacatg atcctttgca aaaccggcct agacaaagaa gccgtgctta ggcgggcagg
     2941 ggctatcatt gttactcttg gaggtttggg ttcccgggtc tttacgccgg gctgccaaat
     3001 ctgcattcct gccgcaaaac cgaagacggt taaggacccc acgggcgcgg gcgattctta
     3061 ccggggaggg ctcatcagcg gtctggtcca gggtaagggc atcgagcagt gcgccagaat
     3121 gggcagtgta tgcgcttcct ttgctgtgga atgctacggc actcaagaat accggttcag
     3181 ctccgatgaa ttcaatgaga ggtttaatgg atatccggga tagggacctg ccttaaccaa
     3241 aagcctggaa tttcggggtg gaggctggtg agcccgatcc aacatccacc cccttccacc
     3301 gagacccttt tcaaaaactc ctattccggg ctccttttat ctttgaccct ccacccgatc
     3361 tctcctttcc gtatagcagt ctaatctttt aaaaagccgt taaatccctc attcctcccc
     3421 cttttccaaa ggagaaaaaa gtggaatctt ccatcgggga ttcccccaaa gctttcctcc
     3481 cttttaaaaa aggaggatgg gagggatttc tgggaaggcc ttttcaaggc gctgaggtct
     3541 tatctttctg ctcattcttg ctttttcccc tcagttttga taaataattc tcagtttcgg
     3601 aagatggaga actcatggaa atcgtggaaa cggacgtttt aatcatcggc gggggaggag
     3661 ccgggatgcg ggccgcgctc gccgcccggg agaagggggc cgaagtgctc ctggtctcca
     3721 aaacgccccc tggaaaatcc acttgcacct atctctccgg cggagccttt tccctggctg
     3781 cagagggatc gaccgttgag acccatttca aacaaactct ccaagcggga aaggggatca
     3841 acgacccgga attggtgaaa gtcctcgtgg aggatgcccc ggaacgggtc cgcgatctgg
     3901 aacgtttggg attgaaggga gcctggggaa aagggcgctt cacttgcctg gggaaggctc
     3961 caagctgggg agcccccctg accgatgcgc tggcggatgc cgcacgggaa ggcggggttg
     4021 gcgagaaagc ctgggtcgcc gtctttgacc ttctgaggga tggggaaaaa gtggtgggtg
     4081 cccttgggtt tgaattccgg aaaggcatac ctttcgcatt tctgagcaaa tcgacggtgc
     4141 tggccaacgg cgggggaggg gccctttacc cccggaacga caaccctgtc cggaccacgg
     4201 gagacgggta tgctttggcc tatgaggcgg gctgcaggct cagagacatg gagtttgtcc
     4261 agttcatgcc cattggactg gccgaagagg gaagtcctcc ttttgtgctg gccccttctt
     4321 tggcagatgc ggggaaggtc gtgaattcct cagggctgga tgtgctggaa aaatatcata
     4381 ttgtggataa acccgtggtc gttcgctgcc gggatacctt ctcccaggcg attatcaaag
     4441 aagaggagag aggagagaag gtcttcctcg acttaaggaa gctaagcgat gaagcctggc
     4501 cgaaagacaa catggccatg agccaaaagc gaatgctccg cgatcgtttc tcctgctccg
     4561 atcgactcct tcggattgcc ccgacggttc atttcttcat gggaggcgtc tgcaccgatt
     4621 ccgatgggcg aacagaagtc ccgggcctat gggccgcagg ggaagtggtc ggcggcttgc
     4681 acggcgccaa ccgcatgggc ggaaatgcgc tgagtgagat catcgttttc ggaagccggg
     4741 caggggcggc ggctgccgaa tgggccttgc gtgagaagcc gctcggggag cccggagcat
     4801 tgatccggtc tcggtgggac cggttccaga aaaaatggaa gagtaacgga aaagggcttt
     4861 ccccgagggt cctgcggcaa cgcatgggag cgatcctctg ggatcagggg ggaatcatca
     4921 gggaagagaa aggattgcag ttaggcctgg aggagctgaa aaagatgaaa accgaagacc
     4981 tcccctgggc cgcctcggag acttcgaagg agattctgga gaagattgaa gtggacaacg
     5041 gcctattggt ttcggaaatg attctccgga gcgccctcat gcgccgggag agccgcggcg
     5101 cccactaccg gaaggacttc gccgtaatgg acgaccggaa ttggaaggga aatatcttct
     5161 tgaggaagtc agcgagtgga atgactctca cctttcaccc tcttccggga aaatcttctt
     5221 gaaaccctat ccgggcatgc taagattagt acgcatagag cagtagaggg tataaccaaa
     5281 gaacaaaaaa caaagacgag ctatcatcta taaatttgtg ggcctttgac tctgcgaacc
     5341 ctgcgtgctc tgcgagagaa tattgtcttc attcttgttg ctggtttctg gttggcagtc
     5401 cccagtattg attttccgac ttcgaatgat ttgatcaagc caccagtaac cttttttgaa
     5461 aaatttaggc atttcgaact ctaggcattt tacttttgcc cccgtagctc aggtggatag
     5521 agcaagggat tcctaatccc tgcgccgcgt gttcgagtca cgccgggggc accaataaag
     5581 tcaaggactt aggcattggc accctccttc aaaatagcct tttcggggtc cac
//
LOCUS       DASPRR010000215         6077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1177998, whole genome shotgun sequence.
ACCESSION   DASPRR010000215 DASPRR010000000
VERSION     DASPRR010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177998"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VEL68_08300"
     CDS             <1..656
                     /locus_tag="VEL68_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HXZ36004.1"
                     /translation="RLALKDPKLKPLAESFRGMKPPRYPSVFEGLVNAITCQQFTLTA
                     GIRLLSRLVVNYGVPFKGRNICFHAFPRPEDLAGAEMEDLRKLGYSRQKARSLIELAQ
                     MVVEGRFHVESLDSLEDSTALERLTALRGVGRWTAEYALLRGMGRIHIFPGDDVGVRN
                     RLQDWMKVARPLGYEGVQRLLKRWKPYGGLIYFHLLLKGLREAGQLPIADCALRVAD"
     gene            complement(668..1264)
                     /locus_tag="VEL68_08305"
     CDS             complement(668..1264)
                     /locus_tag="VEL68_08305"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXZ36005.1"
                     /translation="MSLETFLGTYGYPALLVGTFFEGETFLVLAGFAASLGYLEVPWV
                     ILVAFAGTMSGDQVFFYLGRRQSALVQSFLAHYPTWQNRVGRVQRIIERYQTALVFIF
                     HFLYGLRIAFLLVIGLSRIPAGRFFVLNFLSALIWAVVVGAVGYLFGSALAMIIGDLK
                     DLESYILGGIAVIGGMFGIFQLLSYRIIKDRKTKRVRR"
     gene            complement(1296..2630)
                     /locus_tag="VEL68_08310"
     CDS             complement(1296..2630)
                     /locus_tag="VEL68_08310"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ36006.1"
                     /translation="MTKILRVTVLITWLLGGSWTLAGEVEAMPPSQRMTLSNQLVLLV
                     SEEHSLPFVTLQMLVDAGSRRDPSGEEGIAHLTAKGLLLGTAQRTEKTINEELDYMGA
                     SLSSSGGRDYATLTLRVLKKDLERGLDLFLEVLTQPVFPPGEVKREIQKTLAAIQAAE
                     DQPEEVAEKAFQENLFLSGPYRHPVEGTKTSLPSLTPETVSLFYRQYYHPNGSILAVA
                     GDITLQEIKSRFIPRLEKWPKGQISNATLANVFAKGPKTVKIDRMLTQASIILGHAGV
                     SRDNPDFYALTVMNYILGGGGFSSRLMEEVRNKRGLAYSVYSFFEPGKFPGSFQISLQ
                     TKNVSAREAISLAREEMKRIQKEPVSEKEIEGAKKYLIGSFPMRMDTQGKLVNFLLQV
                     EYYGLGLDYPERYAQLIQGVAREDVLRVARKYLQPENCILVVVANQKMADIE"
     gene            complement(2643..4043)
                     /locus_tag="VEL68_08315"
     CDS             complement(2643..4043)
                     /locus_tag="VEL68_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ36007.1"
                     /translation="MIKSPNLVQYGLWVGLLLVFFSWAPAGQAELREQAFETTLANGL
                     KVILLENHKAPLATVHVWYRVGSRNEEWGQTGLSHMLEHMMFKGTKKYRPEEYSRIIQ
                     ENGGETNAFTTRDYTGYFATLRSDRVQVVMDLEADRMQNLNLREEDFATERQVVMEER
                     RMRTEDDPQSFLLEQLEAAAYQLQPYHWPIIGWMEDIQRFTLENLKTYYRTYYNPANA
                     FLVVVGDFKKEELLPRVEKTFGGIPRGEIPNPNRPTDPQQSGERRISVKKEAQLPFVV
                     IGYHVPNLGQQESYVLEVISALLSGGKSSRLYRRLVQEKQLVLEADAENSILSRDPSL
                     FYLYATPLPGKESADVEKALEEEVERLQKEPVENRELEKVKNQLASSFIYGQDSLYFQ
                     AVLLARYEIVQTWRAIDQYLPAIQRVTPEDIQKVARRYLNPDNRTVGTLVPLPPKEGP
                     AAPAGPAGRGAGGPVR"
     gene            complement(4245..5492)
                     /locus_tag="VEL68_08320"
     CDS             complement(4245..5492)
                     /locus_tag="VEL68_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXZ36008.1"
                     /translation="MKGFGKHGAWVGGFEIFKYIGPGFLVTVGFIDPGNWAANMAAGS
                     TYGYSLLWVVTLSTIMLILLQHNAAHLGIVTGLCLSEACAKYLRGGVRQIVLMSAVGA
                     SISTALAEVLGAAIGLQMLFGLPLSVGAVLTCGLVLWMLFSNSYPRLEKWILGFVSLI
                     GLAFLLELTMVKIPWAEAAHDWVIPSIPAGSLPIVMSVLGAVVMPHNIFLHSEVIQSR
                     QWNVRGEEVIQKQLKFEFADTLSAMIVGWAINSAMILVAASAFHAHGVAVTELAQAQA
                     TLKPLLGNAAAVLFAMALLFSGFSSSITAGMAGGSIFAGIYQEPFNMSDSHSRIGVYV
                     TLFLALAIIFFLRDPFQGLLWSQIILSIQLPFTVIPLILLTSSSIVMGKYANSLRDKV
                     LLWGVASAVIFLNVQLLIQLVRG"
     gene            complement(5567..>6077)
                     /locus_tag="VEL68_08325"
     CDS             complement(5567..>6077)
                     /locus_tag="VEL68_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36009.1"
                     /translation="IKGLSPSHAGSILLCQPVVQALFSPFAGRLSDRIEPRVVSSLGM
                     GFSVMGLFLLVFLGGRTDVVYIVGCLALLGFGFGLFSSPNTNAVMSAVDKKFYGVSSG
                     TVATMRLVGQMLSMGLATLLFALYIGRVQITPEFFPAFLKTMRIAFGIFAILCTAGIF
                     ASLARGRVR"
BASE COUNT         1346 a         1764 c         1709 g         1258 t
ORIGIN      
        1 accgccttgc cttaaaagac ccaaagctaa aacccctggc cgaaagcttc cgggggatga
       61 aaccgccgcg atacccctct gtcttcgaag gcctggtcaa tgccatcacc tgccagcagt
      121 tcacccttac cgccggcatc cggctcctca gccggctggt tgtcaactat ggagttccat
      181 ttaaagggcg aaacatttgt tttcatgctt tccctcggcc ggaggacctt gccggagcag
      241 aaatggaaga cctgcgaaag ctggggtaca gccgccagaa agcccgctcc ttgatcgaat
      301 tagctcaaat ggtggttgaa gggcgattcc atgtggaatc tctggattcc ctcgaagatt
      361 cgacagcttt ggaaaggctg accgcccttc gcggggtagg ccgatggacg gccgaatatg
      421 ccctgctgcg gggaatgggg cggatccata tcttccccgg ggatgatgtg ggggtccgca
      481 accggctgca ggactggatg aaggttgcca gaccgctggg ttacgaaggg gtgcaacgtc
      541 ttcttaaacg atggaaacct tacggggggc ttatctattt tcacttgctg ctcaaggggc
      601 tgagggaggc gggacaactt ccgattgcag attgcgcatt gcgagttgcg gattgaaaag
      661 cgggaaacta tcggcggacc ctctttgttt ttctgtcttt tatgatccga taacttagca
      721 attggaaaat tccgaacatc cccccgatca cggcgatccc ccccaggata tagctttcca
      781 ggtccttcaa gtccccgata atcatcgcca gagcgctccc gaataggtaa cccaccgcac
      841 ccacgaccac ggcccagatc agtgcgctga ggaaattcag gacgaaaaat cttcccgctg
      901 ggattcggct taatccgatg accaggagga aagcgatccg aaggccgtaa agaaagtgga
      961 agataaaaac cagggcggtt tggtatcgtt cgatgatcct ttggactctt ccgacccgat
     1021 tttgccaggt gggataatgg gccaggaaag actgaaccag ggcgctttgc cgccggccca
     1081 gataaaaaaa cacctggtcc ccggacatag tcccggcaaa ggcgaccaga atcacccagg
     1141 ggacctcgag gtatcccagg gaagcggcga acccggccag gaccaaaaag gtttctccct
     1201 cgaaaaaggt ccctaccaag agggcgggat agccataggt ccccaagaaa gtctctaagc
     1261 tcaccggttc ggaaaacccc tttaagctag gagagctatt cgatatccgc cattttctga
     1321 ttggctacta caacgagaat gcagttctcc ggctgaagat acttccgggc gacgcggagc
     1381 acatcttccc gagcaacccc ctggatgagt tgggcgtatc tttccggata atccaatccc
     1441 aggccataat actccacttg gaggagaaaa tttaccaatt ttccctgcgt atccatccgc
     1501 atgggaaaac taccaatcag atattttttg gctccctcga tctccttttc tgaaacgggc
     1561 tccttctgga tcctcttcat ctcctcccgg gcaagggaga tagcctccct tgcagagacg
     1621 tttttagtct gaagactgat ctggaaggaa ccgggaaatt tcccgggttc aaaaaagctg
     1681 tataccgaat acgccagacc cctcttgttg cggacctcct ccatgaggcg ggaagaaaat
     1741 ccacctccgc ccaagatgta attcatcacc gtgagggcgt agaagtccgg gttatcccgg
     1801 ctgaccccgg catgacccag aatgatactg gcttgggtca gcatccggtc gattttcacc
     1861 gtcttgggtc ctttggcgaa gacattggcc agcgtcgcgt tggagatttg gcccttcggc
     1921 catttttcca accggggaat gaacctgctt ttgatctcct gcaaggtgat atccccggct
     1981 actgccagga tggacccatt gggatgataa tattgtcggt agaagaggga aacagtctcc
     2041 ggggtcaggg aggggagaga ggtcttggtc ccttcaaccg gatggcggta ggggccgctc
     2101 aggaaaaggt tctcctggaa ggccttctcc gccacctctt cgggctggtc ttccgccgcc
     2161 tggatggcgg ccagggtctt ctgtatctcc cgtttgacct cccccggggg gaagaccggt
     2221 tgggtgagaa cctctaggaa gagatccagt cctctctcca ggtccttctt taaaactcgc
     2281 agcgtgaggg tggcataatc tctacccccc gaggaactca gcgaagcccc catgtaatcc
     2341 agctcttcgt tgatggtctt ttccgtccgc tgggccgttc ccaggagaag gcccttggcg
     2401 gtcaagtggg cgattccttc ttcgcccgaa gggtctcttc gcgaaccggc atctacgagc
     2461 atttgcaggg tgacgaaagg cagggaatgc tcctcggaga ccaaaaggac cagctggttg
     2521 gagagagtca ttcgctgcga gggaggcatg gcctccacct cgccggcgag cgtccaggat
     2581 cctcctagga gccaggtgat caggaccgtt actctaagaa tcttcgtcat ggtgaaacct
     2641 ccctaacgta ccggaccgcc cgctcccctg ccagctggac cagccggggc cgccgggcct
     2701 tctttcgggg gtagaggaac cagggttccc acggtccggt tgtccgggtt caagtatcgg
     2761 cgggctacct tttggatgtc ttccggagtg accctctgga tcgccggcag atattggtcg
     2821 atggccctcc aggtttgaac aatttcataa cgggcgagaa gcacggcctg aaagtataga
     2881 gagtcctgac cgtagataaa agaggaggcc agctggttct tcactttttc cagctcccgg
     2941 ttctcaaccg gttctttctg caagcgctcg acctcctctt cgagagcctt ctccacgtcc
     3001 gccgactctt ttcccggcag gggggtggcg taaaggtaga acagggaggg gtcccgggag
     3061 aggatggagt tttccgcgtc cgcctccaga accagctgct tttcctggac cagtcgccgg
     3121 taaagccttg aactcttccc cccggacagg agggcgctga taacctccag cacgtacgat
     3181 tcctgctggc cgaggttggg gacatgatac cctatgacca cgaagggcag ttgggcttcc
     3241 ttctttactg agatgcggcg ttctccgctc tgttgaggat cagtgggccg gttgggattg
     3301 ggaatctccc cccggggaat gccgccgaag gtcttctcga cccgcggcag taattcttct
     3361 ttcttgaaat cccccaccac taccaggaag gcattggccg ggttatagta ggtccggtaa
     3421 taggtcttca ggttctccag ggtgaaacgt tggatgtctt ccatccatcc gatgatgggc
     3481 cagtggtagg gctggagttg gtaagccgcg gcctccaact gttccaggag gaaggattgg
     3541 gggtcgtcct cggtgcgcat tctccgttct tccatcacca cctgccgctc ggtcgcgaaa
     3601 tcttcttcgc gcaggttcag gttctgcatg cggtccgcct ccaggtccat gaccacctgg
     3661 acccggtcgg acctcagggt ggcaaagtac cccgtataat cccgggtggt gaaggcgttg
     3721 gtttctcccc cattttcctg gatgatgcgg gagtattcct ctggccggta cttcttcgtg
     3781 cctttgaaca tcatgtgctc gagcatgtgg gaaagcccgg tttggcccca ttcttcgtta
     3841 cgggacccga cccgatacca gacgtggacg gtggccaggg gggccttgtg gttttctagc
     3901 aggatcacct tcaggccgtt tgcgagggtg gtttcaaagg cctgctccct gagttccgct
     3961 tgcccggcgg gcgcccagga aaagaagacc agtagcagcc cgacccacaa cccatattgg
     4021 actagattcg gtgatttgat cattcttcac ttcctttccg attgctgcgg ctgcacatct
     4081 tggtttcccg tccttccttc cccttgggaa tatcgtactc ccaaaccccc ttttttctcc
     4141 aacagagtcc ccctcccgca ggcggactga cttctggctc ttgaaaagaa actccggctc
     4201 ccctgttcat gttcatgtcc aagcggagat cttaggattc cctttcatcc tctcaccaac
     4261 tggatcaata actggacgtt caaaaaaatc accgccgaag caacccccca gagtagaacc
     4321 ttgtcgcgaa gggaattggc gtatttcccc atcacgatgg aagaggaggt cagaaggatc
     4381 agcggaataa ccgtgaaggg gagctggata ctcaggatga tctggctcca cagaagcccc
     4441 tggaaaggat cccggaggaa gaagatgatt gccagggcca ggaaaagggt gacgtaaacc
     4501 ccgattctgg aatgactgtc cgacatgttg aagggttcct ggtagatccc ggcaaagatg
     4561 ctgcccccgg ccattcccgc ggtaatggaa gaggaaaaac ccgagaagag cagggccatg
     4621 gcgaaaagga ccgcggccgc gtttcccagc aaaggtttaa gggttgcctg ggcctgggca
     4681 agctccgtga ccgccacccc atgggcatga aaggccgagg ccgccaccag aatcatggcg
     4741 ctgttgatgg cccatcccac aatcatggct gaaagcgtgt ccgcgaattc aaacttcaac
     4801 tgcttctgga tgacctcctc cccccggaca ttccactggc ggctctggat gacctcggaa
     4861 tgcaaaaaaa tgttgtgggg catcaccacg gcgcccagga cgctcatcac gatggggagg
     4921 gagccagcgg ggattgaggg aatcacccag tcatgggccg cttcggccca ggggattttg
     4981 accatagtca gctccagcag gaaggccagc ccgatgaggg acacaaaccc taggatccac
     5041 ttctccagcc ggggatagct gttggaaaag agcatccaga ggaccaggcc acaggtcaga
     5101 accgccccga cgctcagcgg caatccaaag agcatttgca gcccgatggc cgccccgagg
     5161 acctccgcca gggctgtgga gatggaagcc cccaccgcac tcatcagtac aatttgcctg
     5221 accccgccgc gcagatactt ggcgcaggcc tccgagaggc ataggccggt tacaattccc
     5281 agatgggcgg cattgtgctg caaaaggatg agcatgatcg tggacaaggt caccacccac
     5341 agaagggagt acccgtaggt ggaccctgcc gccatgttgg ccgcccagtt ccccgggtcg
     5401 atgaaaccca ccgtgaccag aaaaccgggc ccgatgtatt tgaagatctc aaaaccaccc
     5461 acccaggcgc cgtgtttccc aaatcccttc atcccaagtc tcccatcggc cttcttcacg
     5521 cccccccagg ccgcctttac gcctccgctg ccggccccgc gcctttttag cggactcgtc
     5581 ccctggccag ggaggcaaaa attccggcgg tacagaggat cgcaaatatc ccaaaggcga
     5641 tgcgcatggt cttcaaaaaa gccggaaaga attccggggt gatctgcacc cggccgatgt
     5701 aaagggcgaa gaggagagtg gccagcccca tgctgagcat ctggcccacc agcctcatgg
     5761 tggccaccgt ccccgaggag acgccgtaaa acttcttgtc caccgcactc atcactgcat
     5821 tggtgttggg ggaagagaaa agcccgaagc caaaacccag cagagccagg cagccgacaa
     5881 tgtagaccac gtcggtccgg ccccccaaaa acaccagaag gaaaagcccc atcactgaaa
     5941 atcccattcc cagggaagag acgacccggg gttcgatccg gtcggagaga cgcccggcga
     6001 aaggggaaaa aagggcctgg acgacgggct ggcaaaggag aatggaaccg gcatgggagg
     6061 gactgagccc tttgatg
//
LOCUS       DASPRR010000216         8642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2307291, whole genome shotgun sequence.
ACCESSION   DASPRR010000216 DASPRR010000000
VERSION     DASPRR010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307291"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1122
                     /locus_tag="VEL68_08330"
     CDS             238..1122
                     /locus_tag="VEL68_08330"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HXZ36010.1"
                     /translation="MPLNVEKLSKALLRGAIDTHIHTAPDIYPRSVTVMEAAKNAKAV
                     GMKAILVKSHCTDTSDRAELTREITGFPVYGGVTLNYPVGGLNIHAVRESIRQGGKQV
                     WMPSTSAIPFLRDAETVPHLAKTLPLGVEGMTILQKNGQLAPEINPILALIAEHDISL
                     ASSHVSPAEGLLLVKAAREAGVKRITITHPHAGFLGYTVDQMIELGRLGAMLEFHYAF
                     ATKMIRNPTPVADLAKSIRKIGPEKCIMATDGGQAGNPPPHEMLRLFIAGMLEAGFSE
                     AEIQIMTIRNPAWVLNLS"
     gene            1351..2115
                     /locus_tag="VEL68_08335"
     CDS             1351..2115
                     /locus_tag="VEL68_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXZ36011.1"
                     /translation="MKIPEKYRKRLMGLIPVDRIVTWQVPRPPRELIQELLGYEGLTP
                     TLSDILDFLGVPGAIPGSEIKPVLQGKKIAGPAVTLKYVPERTTPAQSFYEKAKAKLA
                     DRDAYAMTEPGDVVVIDGGGRGEISAMGGLSTLVAKKYGLAGNIVDAGVRDIQDIRGL
                     DYPVWSRGITPITGKFRFEAREINGPVVCAGVSVHPGDLVVADDTGIVIVPQELIKEV
                     IRLAIEASKKEERLIQVIEEGSSIEEMKKVLAPEKW"
     gene            2212..3261
                     /gene="vorB"
                     /locus_tag="VEL68_08340"
     CDS             2212..3261
                     /gene="vorB"
                     /locus_tag="VEL68_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HXZ36012.1"
                     /translation="MAFELMRGNEAVAEGAIRAGCRFYTGYPITPQNELFEYMAKRMP
                     QAEGTFLQCESEVAGINMIWGAACTGMRAMTSSSGPGVSLMSEGLTTLAAGQLPCVIV
                     DVTRAGPGLGRIAPAQSDYRQVTKGGGHGDYHAIVLGPSSTQEMAELANLAFYLADLY
                     RNPVIILMDGMLGQMMEPVDFDQVKPLPIPEKPWALRGKGEGPRKMVYAAPFDDPGLI
                     QLNQELEEKYRRIEEKEQRWEEVEVEGAELVVVAFGSAARASLDAVQTAQAEGMKVGL
                     VRPITLWPFPKKAFSRLRRSAKAFLDVEMNAGQMQEDLERVIGPDFPVYHLGQGGGKI
                     IYPDEVLEEIKKLMK"
     gene            3335..4075
                     /locus_tag="VEL68_08345"
     CDS             3335..4075
                     /locus_tag="VEL68_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ36013.1"
                     /translation="MKKIFGKPDALTDFPYPYCPGCGHGIVQRLVGEAMDQLGIVGKA
                     IGVTSAGCSVRNWKQFACDMTMGLHGRGPAVATGLKRTAPECIVFTYQGDGDMAAIGT
                     GEIVHAASRCERITVIFVNNAVFGATGGQQAPTTLLGQKTTSFPGGRDPRTGGYPLRM
                     SEMLSLVSPESYIVRVSLHDVKHINQAAKATRRAFEVQMNNEGFSMVEYLSMCPTQWK
                     VTPVQALNWVEEKMIPYYPLGEYGTIRN"
     gene            4179..4721
                     /locus_tag="VEL68_08350"
     CDS             4179..4721
                     /locus_tag="VEL68_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ36014.1"
                     /translation="MHESVIMSGFGGQGILLMGQLLCYAGMHEGKNVTWMPSYGPEMR
                     GGTANCTVMLSSDRVGSPVTRYPFSLVAMNKASLDKFENAIQPNGFLVINSSLVDREP
                     TRTDLKYIKVPANRIAEEAGTLQVTNMAALGAYVVGRGVVSLDSVLIALRRMIPEHRK
                     ELLSVNEEALRRGAEAAKKQ"
     gene            4845..5048
                     /locus_tag="VEL68_08355"
     CDS             4845..5048
                     /locus_tag="VEL68_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXZ36015.1"
                     /translation="MGKIAIDKERCKGCQLCIVTCPKKSLRLSEEFNDKGYHFIIFED
                     GSECTSCTFCGRVCPDMAIEVYK"
     gene            5312..6598
                     /locus_tag="VEL68_08360"
     CDS             5312..6598
                     /locus_tag="VEL68_08360"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXZ36016.1"
                     /translation="MNFPRIYRIRQAFDRTQVQDIPGTVKGELKKLSLEKKVKPGQRV
                     ALTAGSRGVANIAVILKAAAEYLKSIGAKPFIFPAMGSHGGAKAEGQTDLLAHYNVTE
                     AFTGAPVLSSMEVIEISTTEDGVPVYIDKNASQADWIIVANRIKPHTKFKAPIESGLM
                     KMMAIGMGKQKGAEYYHKAAIQFTFPKIIVDAGREVIKKAPILCGLGMVENGYDETAK
                     IAAILPQEIEARERELLVLAKQMMPRLPFNEVDLLIVDEMGKDISGTGIDPNVTGRNR
                     DLLGVFPHPVNVKRLFVRDLTENSNGNATGLGLADLTTKRLVDKINYQATYMNCITGI
                     SIEKAAVPMHFPTDQEAIQIALGSIGLTPSEKSRIVRIKNTLHVDEVEASEAYLEEMK
                     KRSDLEALEGPRPMSFDGQGNLHPLKVHGGGRKADF"
     gene            6727..7812
                     /locus_tag="VEL68_08365"
     CDS             6727..7812
                     /locus_tag="VEL68_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HXZ36017.1"
                     /translation="MIDIFLGPKQYIQKEGLLGESGNHLKPFGRRPMVLGDELVFSII
                     RPTLEDRLKSAGLSPFFVLFGKECSMAEISRLVEIVRKENLDFIVGTGGGKSIDTSRM
                     VAEQLKFPLITIPTSAATCSAASAAAVVYEKGIRQATVNGKGADLVLVDSTILSQAPT
                     RLLASGMGDALAKWYEGKPCYDRMKEPDSATQSALSLSTQTKEAILAVGLEAKKDVEA
                     KRNSYAVEKVVEANVLLTGVIGGLGGSKFRVAVSHALLYGLTVLPQVHQNLHGEMVSF
                     GIIVQLCLEKNEKELKTLLPFFSRLGLPITLKSLGLTNVEDPLFWEGLKRTCVKGSSV
                     HNMPFLVDEQKLYRGMLEADERARAFR"
     gene            7904..8440
                     /locus_tag="VEL68_08370"
     CDS             7904..8440
                     /locus_tag="VEL68_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ36018.1"
                     /translation="MKRYEIRIAGFGGQGVVTVGKIVGVAAALYDKKNAVQTQSYGPE
                     SRGGACKSEVVLSDGEIHYPKVRMADVLVALSQPALDIYLKDLKAGGILIIDPLTVIK
                     EVPRADIKVFKVPTAEIALQVGNKKFQNMVALGALASLTGVISPEALEKAIKESVPPK
                     TLNQNLEAFKKGMGYVRS"
     gene            8427..>8642
                     /locus_tag="VEL68_08375"
     CDS             8427..>8642
                     /locus_tag="VEL68_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36019.1"
                     /translation="MSEVEDSAFEPLMTEEEAKKVLHGHLPPRDYYLFGSDACVEGAI
                     FAGCRYYAGYPITPASEIMEKAAQRMPQ"
BASE COUNT         2048 a         2347 c         2482 g         1765 t
ORIGIN      
        1 aagtcctacg acaaactccg caaaaaggcg tcttcccctg acctcctttt ccccggacac
       61 gacccgctga tgagctccaa tttcccaaag gtagccaagg atgtaacccg attggtttga
      121 tcgccactcg aataaaaatt ataatcgtaa actcgaagag cggttcattg cctagccgcc
      181 aaatccagga ctgggagaaa ccactcagtc tcgactctag ccaaaaggag gaatgttatg
      241 cccctcaatg tggaaaaact ttcgaaagcg ctgctacgag gtgcgatcga cactcatatc
      301 cacaccgccc ccgatattta tcctcgctcg gtaaccgtta tggaggccgc caagaatgcc
      361 aaagctgtgg ggatgaaggc tatcttggtg aaaagccact gcaccgacac ttccgaccgt
      421 gcagagttga cccgcgagat aactggcttt ccggtctatg ggggagtcac tctcaactac
      481 ccggtcggtg ggttgaatat ccatgcggtg cgcgagtcga ttcgtcaggg gggcaagcaa
      541 gtctggatgc ccagcaccag cgccattccg ttcctgaggg atgcggagac ggtgccccat
      601 ctggccaaga cccttccgct cggcgtggaa ggaatgacca ttctgcagaa aaacgggcaa
      661 ctcgcgccgg aaatcaatcc cattctggcg ctgattgccg aacatgatat ttccctggcc
      721 tcttcccatg tctcccccgc ggaaggcctt cttcttgtta aggcggctcg cgaagccggg
      781 gtgaaacgga tcaccatcac tcatccccac gccggtttcc ttggctatac cgtggatcaa
      841 atgatcgagt tgggccggtt gggagccatg ctggagttcc attatgcctt tgccaccaaa
      901 atgattcgga acccgacgcc ggtggccgat ttggccaaaa gcatccgaaa aattggcccc
      961 gaaaaatgca tcatggccac ggatggcggc caggcgggga atcctcctcc ccatgaaatg
     1021 cttcgcttat tcattgccgg aatgttggaa gcaggtttct cggaggcgga aattcagatc
     1081 atgactatac gcaacccggc gtgggtccta aatttgagtt gaagcccaat gcaacagacc
     1141 ctcttcacgt accgaaatct gtaccccctt tcgattatcg gttccagaaa gaggagcagc
     1201 ccgtttctta aaccctgaaa aattcaagac ttccttcgct aaaagaacaa agaaggacgc
     1261 atgatttttc tctttgacaa accttcctgg gccgggttag aaataggtgg gcctttatct
     1321 cttatccaaa ctctctaagc gagggaaatt atgaagattc cggagaaata ccgcaaacgg
     1381 ttgatggggt tgatcccggt ggatcgaatc gtcacctggc aggttccccg gccgcctcga
     1441 gaactcatcc aggaactcct cggctatgaa gggcttactc ccaccctttc cgatatcctc
     1501 gacttcctgg gtgtcccggg ggcaatcccc gggtccgaga tcaaaccggt tctccagggg
     1561 aagaaaatcg ccggccctgc ggttaccctg aagtacgtcc ccgagcgcac gactccggct
     1621 caatcctttt atgaaaaggc caaggctaag ctggccgacc gggacgccta tgccatgacc
     1681 gagccagggg atgtggtggt catcgacgga ggagggaggg gggagatctc cgccatggga
     1741 ggactttcca ctctggtggc caaaaagtat ggtttggcgg gaaacatcgt ggatgccggg
     1801 gtccgggata ttcaggacat ccgcgggctt gactacccgg tgtggtcccg ggggatcacc
     1861 ccgattacgg gaaaatttcg ctttgaggct cgggaaatca acggcccggt ggtctgcgcc
     1921 ggggtctccg ttcatcccgg cgatctcgtg gtggcggacg acacgggcat tgtgatcgtt
     1981 ccgcaagagt tgataaaaga ggtaatccgg ctggcgatcg aggcctctaa aaaggaggag
     2041 cgcctcatcc aggtgatcga agaaggctct tcgatagagg agatgaagaa ggttctggcg
     2101 ccagaaaaat ggtgagaaaa ggttagaggt tggaggttag ggatgaggct attgcctcaa
     2161 acctctaacc tccaacctct aaccttttac ccgtattttg ggaggctttg aatggctttt
     2221 gaactgatgc gcgggaacga ggcggtagcc gagggggcca tccgcgccgg ctgccggttt
     2281 tacaccggct atcccatcac ccctcagaat gaacttttcg aatacatggc caagcggatg
     2341 ccgcaggcgg aagggacttt cctccagtgc gaaagcgagg tggccggaat caacatgatc
     2401 tggggagcgg cctgcacggg aatgcgggcc atgacttctt cttccggccc cggcgtgagc
     2461 ctcatgtccg aagggttgac caccctggcc gccggacagc tcccctgtgt catcgtggac
     2521 gtgacccggg caggtccggg gctgggccgg attgctcccg ctcaatcgga ctaccgtcag
     2581 gtgaccaaag gcggagggca cggggattac catgccatcg tccttgggcc ttcttcaact
     2641 caggagatgg ccgaattggc gaacttggct ttttatctgg ccgaccttta tcgcaacccg
     2701 gtcatcatcc tcatggacgg gatgctggga cagatgatgg agccggtaga ttttgaccag
     2761 gtgaaacccc tcccaatccc ggagaagccg tgggctttgc gcggaaaggg ggagggaccc
     2821 cgcaagatgg tctatgccgc tcccttcgac gatccgggct tgattcagct gaatcaggaa
     2881 ttggaagaaa aataccgccg aatcgaggag aaggaacagc gctgggaaga agtggaggtc
     2941 gagggggctg aactggtcgt ggtcgctttc ggcagcgcgg cccgtgcctc tctggatgcg
     3001 gtgcagaccg cccaggccga agggatgaag gtgggtttgg tccgtccaat tactctatgg
     3061 cccttcccca agaaggcctt cagcagactt cgccgttcgg ccaaggcctt cctggatgtg
     3121 gagatgaacg ccggtcagat gcaagaggac ctggagcggg tcatcgggcc cgattttccc
     3181 gtctaccatc tggggcaagg tggagggaag atcatctatc cggatgaagt cttggaagag
     3241 atcaaaaagc tgatgaaatg aattttcccc cccccgccct cgccctcccc ctcgaggggg
     3301 agaattggga ggaggcggga tggaggtttt ttgaatgaag aagatttttg gaaagccgga
     3361 tgctttgact gacttcccct acccctactg tccaggctgc ggtcacggga tcgtccaacg
     3421 cttggtgggc gaggccatgg atcagctggg aatcgtgggc aaggcaatcg gagtgacttc
     3481 ggccggctgt tcggtgagaa actggaaaca gtttgcctgc gacatgacca tgggattgca
     3541 cggccggggt cctgctgtgg caacgggttt gaaacgtacg gccccggagt gcatcgtttt
     3601 cacctaccag ggagacggag acatggctgc catagggacc ggagagatcg ttcacgcggc
     3661 atcccgctgc gagcgaatca cggtgatctt cgtcaacaat gctgttttcg gggccaccgg
     3721 aggacagcag gcccccacca ccctcctggg ccagaagact accagttttc ccggagggcg
     3781 ggacccacgg accggagggt atccgctgag aatgtccgaa atgctttccc tggtttcacc
     3841 ggagtcctac atcgttcggg tctcccttca cgacgtgaag cacatcaacc aagccgccaa
     3901 agccacccgc cgtgcttttg aagtccagat gaacaacgaa gggttctcca tggtggaata
     3961 cctctccatg tgccccaccc agtggaaggt gaccccggtc caggcgttga attgggtgga
     4021 agaaaagatg atcccctatt atccgttggg ggaatacgga acgataagaa attagcgatc
     4081 agcggtcagc tctcagcctc cagctaaaat ccaaaaacaa aaggttttgg ctgatggctg
     4141 aaagctgacc ggtggtcgat gcttttattg gagtttttat gcatgaatcg gtgattatgt
     4201 ccgggttcgg ggggcaggga atcctgctta tgggccagct cctctgttac gccggaatgc
     4261 acgaggggaa aaacgtgact tggatgcctt cctacgggcc agagatgcgc ggggggacgg
     4321 cgaattgcac ggtgatgctc tcttccgacc gagtgggctc tcccgtaacc cgctatcctt
     4381 tctccctggt ggccatgaat aaagcctccc tggataagtt tgagaacgcc atccagccga
     4441 acggatttct ggtcatcaat tcgtccctgg tggaccggga gcctacccgc accgatctca
     4501 agtatatcaa ggtaccggcc aataggatcg ccgaagaggc gggcaccctg caggtgacca
     4561 acatggccgc gttgggagcc tacgtggtcg gtagaggagt tgtttcgctg gactcggtcc
     4621 tcatcgccct gcggcgaatg atccccgagc accggaagga attgctatca gtgaacgagg
     4681 aggccctgcg ccgcggcgcc gaagccgcca aaaaacaata ggacgcggat gaacgcagat
     4741 acacgcagat aagaaaagaa atatttgaca ggaaaaaaca aaaaataatt atcctgaaaa
     4801 tctgcgttca tctgcgtccc attcatgttt gaacggaaaa agaaatggga aagattgcga
     4861 ttgacaagga acgctgcaaa ggatgccagc tctgtatcgt gacctgtccg aagaagagcc
     4921 tccgcctttc agaggagttc aacgacaagg gatatcactt cattattttc gaggatggaa
     4981 gtgaatgcac gtcctgcacc ttctgcggac gggtctgtcc ggatatggcc attgaagttt
     5041 acaaatagaa aatacgcaaa gcgcagaggg cacattgcgg cgtagccgca accaaaagaa
     5101 atcgtttact tctaactcat gagtcaagga atcaccccct ccctgccctc ccccctcgag
     5161 ggggagggtc agggtggggg gggatgtttt ttggttaaca aaatctaaga ttctgcatgt
     5221 tagtagtata gagcatagag aaaaaaatct ttagtcttta ttatgccatg ctctatgcgc
     5281 tatgccctgt gcatctcgta aggaggttgt tatgaatttt ccccgcatct accgaatccg
     5341 ccaggctttc gatcgaaccc aggtccagga tattccggga acggtgaagg gcgagttaaa
     5401 gaaactctcc ttggagaaaa aggtcaaacc ggggcaacgc gtggcgctca ccgcgggaag
     5461 ccggggggtg gccaatatcg ccgtcatcct gaaagcggcg gcggaatacc tcaaatccat
     5521 aggggccaag cccttcatct tccccgccat gggcagccat ggcggagcta aggctgaagg
     5581 gcagacggac cttctggctc attacaacgt caccgaagct ttcaccggtg cgccggtcct
     5641 ctcctccatg gaggttatag agatcagcac gaccgaagac ggggttccgg tctacatcga
     5701 caagaacgcc tcccaggcag actggatcat cgtggccaac cggatcaaac cgcataccaa
     5761 attcaaagcc cccattgaaa gcgggctgat gaagatgatg gccatcggca tgggaaaaca
     5821 gaagggcgcc gagtactacc acaaggccgc catccagttc accttcccga aaatcatcgt
     5881 cgatgcggga cgagaggtga tcaagaaggc gcccattctc tgcggtctgg ggatggtaga
     5941 gaacggatac gacgaaacgg ccaaaattgc ggccatcctc ccccaggaga tcgaagccag
     6001 ggagagggaa ctcctggtac tggccaagca gatgatgccc cggctgccct tcaacgaggt
     6061 cgacctgctc atcgtggacg agatgggcaa ggacattagc ggcacgggaa tcgatcctaa
     6121 cgtgaccggc cgcaaccgcg accttttggg cgtcttcccc catccggtca acgtgaagcg
     6181 cctctttgtg cgtgacctga ccgaaaactc caacggcaac gccaccgggc tggggctcgc
     6241 cgacctgacc accaagaggt tggtggacaa gatcaactac caggcgactt acatgaattg
     6301 cattaccggg atcagcatag aaaaagccgc tgtgcccatg cacttcccca ctgatcagga
     6361 agccatccag atcgctctag gatcgatcgg cttgactccc tcggagaaaa gcaggattgt
     6421 ccggatcaag aacaccctgc atgtggatga agtggaggcc tccgaagcct acctggaaga
     6481 aatgaagaaa cgatccgacc tggaggccct ggagggtccc aggcccatgt ctttcgacgg
     6541 ccaggggaat ttgcatccct tgaaggttca cgggggcggg cgaaaggcgg atttttaaaa
     6601 gagagttgat ggttgctcgt tgctagttga agaaaagccg gccggagcaa accatacccc
     6661 ggagcgactg accaggaacg agaaaccagt aactagcgac cagcaaccag gaacaaggaa
     6721 cgaaccgtga tcgatatttt tcttggcccc aaacaataca tccagaaaga aggattgttg
     6781 ggcgagtccg gaaaccacct gaaacccttc ggacggcgcc ccatggttct gggagacgag
     6841 ctggtctttt ccatcattcg acccaccctg gaggaccgtc tgaagtcagc gggactttcc
     6901 cctttctttg tcctcttcgg caaagagtgc agcatggccg agatttcccg cctggtcgaa
     6961 atcgtcagga aggagaacct ggatttcatt gtgggcaccg ggggtgggaa atccatcgac
     7021 acctcccgca tggttgccga gcagctgaag tttcctctga tcaccatccc caccagtgca
     7081 gccacctgtt cggcggcctc tgcggcggcc gtcgtctacg aaaagggaat ccgccaggct
     7141 acggtgaacg ggaaaggggc ggacctggtc ctggtggatt ccacgatctt atcccaggcc
     7201 cccacccgtt tgctcgcttc aggcatgggc gacgccctgg ccaagtggta cgaggggaag
     7261 ccctgttacg accggatgaa ggaaccggac tccgcgaccc aatctgcgct gagcctctcc
     7321 actcagacca aggaggcgat tcttgccgtg ggcctggagg ctaaaaaaga tgtggaagcc
     7381 aaaaggaatt cctatgccgt ggagaaagtc gtcgaagcca acgttctcct gaccggagtc
     7441 atcggaggtt taggagggtc caagttccgt gtggccgtgt cccatgccct gctctacggc
     7501 ctgaccgtcc ttccccaggt ccatcaaaac ctccatggag aaatggtatc ctttgggatc
     7561 atcgttcagc tctgcctgga gaagaacgaa aaagagctga agaccctcct gccctttttc
     7621 tcccggctcg gcctgccgat aaccttgaag agtctcgggc tcaccaatgt ggaagacccg
     7681 ctcttctggg aagggcttaa acgaacctgc gtcaaaggca gctccgtgca caacatgccc
     7741 ttcctggtgg acgaacagaa gctttatcgg ggcatgcttg aggcagacga acgggccagg
     7801 gcatttcgtt aaatgtcatc tcgcaggtcc tcccttttcc tcttttgtcg aaaggggttg
     7861 gagggattcc aatgatcaga ttggaccaat aaaaggggaa aaaatgaaac gctatgaaat
     7921 ccgcatcgcc ggattcggcg ggcaaggggt ggtgaccgtg ggaaagatcg tcggggttgc
     7981 cgccgccctt tacgataaaa aaaatgcggt tcagacccag tcttatggcc cagaatcgcg
     8041 gggtggagcg tgcaagtcgg aagtggtact ttcagatgga gagatccatt accccaaagt
     8101 tcgaatggcg gatgtattgg tcgctctatc ccagcccgct ctggacattt accttaagga
     8161 tttgaaggcg ggagggattt taatcatcga tccgttgacc gtgatcaaag aggtcccccg
     8221 ggccgatatc aaagtgttca aagtcccgac ggcggaaatc gccctgcagg tgggaaacaa
     8281 gaaattccag aacatggtcg ccctcggcgc tctggcctcg ctgactggcg tgatttctcc
     8341 agaggctctg gaaaaggcca tcaaggagtc tgttcccccc aagactctga accagaacct
     8401 cgaggccttc aagaaaggaa tgggttatgt ccgaagttga agattccgcc tttgaacccc
     8461 tcatgaccga ggaggaggcc aaaaaagtcc tgcacggcca cctccctccc agagactatt
     8521 acctcttcgg cagcgacgcc tgcgtggaag gggccatctt tgccggctgc cggtattatg
     8581 ccggctatcc catcactccg gcatcggaga tcatggaaaa ggccgcccag aggatgcccc
     8641 ag
//
LOCUS       DASPRR010000217        14736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2145913, whole genome shotgun sequence.
ACCESSION   DASPRR010000217 DASPRR010000000
VERSION     DASPRR010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14736)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14736
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145913"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..249)
                     /locus_tag="VEL68_08380"
     CDS             complement(82..249)
                     /locus_tag="VEL68_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36020.1"
                     /translation="MICKKILLINENGTQVWLEQKSNGSLWQNGEEIFSSEDEAKQKG
                     WKVDAVKIIFE"
     regulatory      complement(518..592)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1207..1716
                     /locus_tag="VEL68_08385"
     CDS             1207..1716
                     /locus_tag="VEL68_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36021.1"
                     /translation="MKTNKTREIFFKMWRFFSRAKGKVSNNPQSFLQLANENQMKENP
                     QLKIAEKYQKQGEKQRPRQENLRSAKLFCDTGKKQKGAAICPKLLKQGSEMELMRECN
                     SDANKDDHNRIVKAPMLLLPDTEISRFFDLAKILEANSSLELGEIRSITIEESYRPEI
                     FLEERKQEI"
     gene            complement(3250..4641)
                     /locus_tag="VEL68_08390"
                     /pseudo
     CDS             complement(3250..4641)
                     /locus_tag="VEL68_08390"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            complement(4806..5513)
                     /locus_tag="VEL68_08395"
     CDS             complement(4806..5513)
                     /locus_tag="VEL68_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36022.1"
                     /translation="MIEVKEITVRYGGITALDRVSLAIPDGKMISIVGANGAGKSTTI
                     NAISGMVPLSGGEIIFEGRSLVISPHLIARLGVVQVPEGRKIFSSITVKENLLLGAYM
                     IHDRKRIEENLKKVYRTFPILEERQVQAGGTLSGGEQQMLAIARGLMSEPRTLLLDEP
                     SLGLAPLMVLGIFKMIETLHRDGLTILLVEQNAQKALIIADYAYVLETGRMVAQGEGK
                     KIAEDPVVKKAYLGIEE"
     gene            complement(5562..6332)
                     /locus_tag="VEL68_08400"
     CDS             complement(5562..6332)
                     /locus_tag="VEL68_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36023.1"
                     /translation="MTAEVLKVTEVSKNFGGVAAVANFSMLQRENEITGIIGPNGAGK
                     TTVFNLITGVYPVDTGEIWFYGKETTRRPAHVITALGITRTFQNIRLFRNLNVLDNLA
                     VAYGVKTHYTVWEEMFRLPRVRREEKEAADLAKSYLSYFGMEKYAAYFPYNLPYGLQR
                     RLELARALMASPRLLLLDEPGAGLNPKEIRELMGTLLKIREEKKLSMIVVEHHMELVM
                     KICTTLHVLDFGKKIAEGRPEEIKKNEQVLQAYLGDEV"
     gene            complement(6329..7180)
                     /locus_tag="VEL68_08405"
     CDS             complement(6329..7180)
                     /locus_tag="VEL68_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36024.1"
                     /translation="MEFYLKVLEITCLHTILVLGAYLMTGLTGLFSLGQGGFLSIGAY
                     TAAIAFLKFNLPFPACLALAVGMGVLVGFVIGLPTLRLHRDYFLLVTFGFGEMVRAAL
                     LHLAQLTGGALGVAGIPKLIGLPTIILSIVVITLITACLKRSRFGRNCIAIRDDELAA
                     TLMGIPVYRHKMTVFILSSAITAYGGALYAFVIMFIEPNLFNWLESAKLIIITFVGGV
                     NSITGAIVTSIVYYSFGEFFRFVDVWRDVLLAFAVLLVTIFRSQGIFGNWELTPGNLL
                     FWRRKAG"
     gene            complement(7253..8137)
                     /locus_tag="VEL68_08410"
     CDS             complement(7253..8137)
                     /locus_tag="VEL68_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36025.1"
                     /translation="MDFSVLLQQLFNGVSLGGVYALEAVGFGLIFNILKFSNFSHSGL
                     VAICAYLGYFLARQVYSNFIVTLILTTVAGGFIGVLIEKLIFRPIRLKGAPQTLFLVN
                     SITAAMLVQQFFSATLGDDYFPYPEFMKQTAFHLGNLTVSKTYLMMLSITAVVLLLLT
                     WVIRRTRIGIALRAASTDIRTPSLMGINVDHIISSAFFMAGVLGGITGYLLGMTYNVD
                     PFIGSMILKGIIASIIGGMGSLGGGVIAGLLLGVAESLLIAEIGSSLTQIAVYGAIFL
                     LLMIRPEGIAGKRYIIKA"
     gene            complement(8305..9480)
                     /locus_tag="VEL68_08415"
     CDS             complement(8305..9480)
                     /locus_tag="VEL68_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36026.1"
                     /translation="MKKSLFSVLFPLAMVLVCAMPSAAQETIKIGMYADLSAGSAQWG
                     NDALKGAKLMVKEANAAGGLLGKKIELIAYDCKMSPTEGVKVYSRLAKEDKVSAVYGS
                     LISNIGLAVSPVAEQVKVPVVARALDERVTTPDFKMEDPENPGRVNPYCFLLQPSSYQ
                     QSYMIAGYAIDELKLNTFAQLFTPSNSYSFMLAKGFEYYVKKRGKKLLGNFEFQAGDA
                     DFKAQLTKVKALGPEGLYIPNYVPENANAAKQVRELAIKTTMLGNNSWYEPMDKVAGA
                     AADGAYFPLNISRDDPSLKPFIDKYNKEYGELPRLHSFSGWDDVGFIIEAIKRCKSTD
                     PKIIRDTMAGTTKYEGLIGTINIDPKTHRPVGLKMSILKFEGDKIKTVLLKYYPKDI"
     gene            9869..10696
                     /locus_tag="VEL68_08420"
     CDS             9869..10696
                     /locus_tag="VEL68_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36027.1"
                     /translation="MAGPKLKYLGLTERDLDFLVETVSPEARDKGRLKQVIREDRDIR
                     NSYVGDEKVFQRLMDEEESFVKISPILFFEVLLRKAAQDLEKAQYTVEKSRTMKIPIF
                     DTQEVVGLLNREDLLFYLADMLSSFTRVESYTIPVRIREGIWERVRFNDLDILSLMRF
                     SQFVDNEHRLGFYKRIADICLFIMGIFPDYAERDYRYPFSGQVRPHVRGKLRISPADY
                     AEEGQKFYKLAAQHPSAREMHLEEIFWALHRNFQKAKKPLNFIADYYLQYRRDRFFG"
     gene            complement(10803..11777)
                     /locus_tag="VEL68_08425"
     CDS             complement(10803..11777)
                     /locus_tag="VEL68_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36028.1"
                     /translation="MGTRKNYRKTSAIILGMAFLLLTSPAFPEFPDKPITIFCGYGPG
                     ASTDVSSRSLASGLEKMWGVPVVVENKPGGGTTVCAGLVASKKPDGYTLGVISEGALT
                     ANPLLQKLAYDPLKDFTFLAQYATYFGSFVVRSDSPWKTLDEFIAHAKAHPGMSYTST
                     GMYTRQQISAELLAQCKGLTFRHVPTKGGTDASTMLMGGHADFSTGSSQIVYVRQGVF
                     RLLVQVNAEKRNPSFPDVPTLREIGCPDVPPAGIDVIAPKGLPPSISQKLGEAIRKVT
                     LEPSFQKVLTSFDVPYDYLDHEGLEKKVRKEYEWFKDYLQKSGIKTTK"
     gene            12851..13312
                     /locus_tag="VEL68_08430"
     CDS             12851..13312
                     /locus_tag="VEL68_08430"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXZ36029.1"
                     /translation="MNKMWAVVAVVLVLGLGFATGSQAKPMSQSGLRIYDTTKLIGST
                     VKSPDGVTLGQIFDLVVDSQGHVDFAIVSQPGFEEFPGRLVAVPFSSLMISEGKSQQA
                     QVVLHADQEKFYEAPSWGDKNLADRQQAASLDRYFGVSPYWPEKTGQAPRQ"
     gene            complement(13595..14074)
                     /locus_tag="VEL68_08435"
     CDS             complement(13595..14074)
                     /locus_tag="VEL68_08435"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXZ36030.1"
                     /translation="MSKKIVIISVLVSFLSLGIEAYSTSQGSLAEKEGLPSTLTDLIG
                     MQVKNPVGEDLGMISDITLDPMGLPFVVLYQGAIEDFDVARYVLVPFSAFSISEAKSP
                     EKTVILNFDRQRLLAAPPYDRSKGMKMRPNQWEEIYRYYGQIPYWKKEGAGWDIASR"
     gene            14345..>14736
                     /gene="lgt"
                     /locus_tag="VEL68_08440"
     CDS             14345..>14736
                     /gene="lgt"
                     /locus_tag="VEL68_08440"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXZ36031.1"
                     /translation="MIKIDLNPILVALGPIRVTWYGMMYVLGFILSYLLVRSQVQKKD
                     FGISLKEVEGLFFDLFLGLIIGARLGYVLFYDLQAYLSNPLEIFAVWHGGMSFHGGLI
                     GVLIAAVLFCRKQKKSFWKLSDLFVVTAP"
BASE COUNT         3436 a         3769 c         4026 g         3505 t
ORIGIN      
        1 ccagccaaaa taagtataaa ccaggaaagt attcctgaaa aagttctgat taaatgttac
       61 tatcccattc aatagatgtg atcattcaaa aatgatcttt accgcatcaa ctttccagcc
      121 cttctgctta gcttcatctt cggaagaaaa tatctcctct ccattttgcc aaagcgaccc
      181 gttactcttt tgctccagcc atacttgcgt cccattttca tttattagaa ggatcttttt
      241 gcaaatcata ttgcttccct ttgggtctta gcaattttca ggatcccgct cttcatttca
      301 aagtattgtc ttacagtcct cattccaagc cgaatcattt tctgggtatc ttaaaaacta
      361 tacccatctg agggttatca ggctgatcca aacagcaacg aaatcagctg ccgaggaaca
      421 aagcccgatg tcaaggcgtc ctttcttcca aaataaaaag ccggaccccc gacggtaaat
      481 tgcacggcgt gagtccggct gcaatcttgc catcgacttc ggcaacccgg ctatccccat
      541 gggacccgtg gctttccgtc ccacgattgc tcgtggtttg gcattgtcgg tcgtcagtgg
      601 aagaccgtac cgcctaattt tacgcggcct tgagtcttcc tatatttccg atctcttttc
      661 ggcaatgcca gggaaaactt tagggaaatt gtggtggcca ttcaaaattc tggcgtttca
      721 aatagaagga aaccaataaa ttcaatttat taaataactc caattggaat tgaattagca
      781 gcaacttgag gtacataaaa ccccaccttt gcgctttggg ccgagcggac ttcaccggcc
      841 tggttccctt caaagatgga aagggggcag ggttctgcaa aggcgcaagg ggtgtcggcg
      901 aggcccagcg ggtgcgcccc gccatgggcg gaacacaccc catgggctga gaatatttct
      961 gctccctttc cacccactcg attgggagaa gagtcttaag actatctttg cgtcgcatta
     1021 tgtcattgga tagattttaa ttgtcaattc actcgaaaag tggggctcga ttcctgtcat
     1081 attcaagctg aaaaccccat atttattgaa taaatcattt taggattgat aggcaattat
     1141 ttacggtatg aagtttgcac cctttctcgg tgtgcggaga aaaatataga atcttccggc
     1201 ttaagaatga aaacaaacaa aaccagagaa atctttttca aaatgtggcg attttttagt
     1261 agggcaaaag ggaaggtatc aaataacccc caatcctttt tgcaactggc taatgaaaat
     1321 cagatgaagg aaaatcctca actcaaaatc gccgaaaaat atcagaagca aggtgaaaaa
     1381 cagagacctc gccaggagaa tctgaggtcc gcgaaactct tttgcgatac tgggaaaaaa
     1441 caaaagggag cagcaatttg cccaaagctc ttgaaacagg gctcagagat ggagttgatg
     1501 agggaatgta attcagatgc aaacaaagat gatcacaatc gaatcgtaaa agcccccatg
     1561 cttcttcttc ctgatacgga aatctcccgt ttctttgatc ttgcgaaaat attggaggca
     1621 aacagctcat tggaattggg agaaatcaga tcaatcacca tagaagaaag ttatagacct
     1681 gagatttttt tggaagaaag aaaacaagag atttagaaag aatataaagc tttgagcaca
     1741 atctccgggg aaaatatgct caccttgtca cagttttcaa accggctatc caagtggtaa
     1801 tgatttttta gggatgggat ttccaggttg atgctataga aatgtctttg acctggttcc
     1861 ttgacctcat caaatcgaag tatttgccag ggcaatatag aggggacact gaacggtata
     1921 attgaaggtc ccagaaaacg gactgcggct gattccgcaa tctgcagccc gatcacattc
     1981 aaatccagcc aaaaatttac ggtccttctt ataaaaatat tctacattaa catgggctat
     2041 gccccccaat ttcttacacg ggtaatccat agattccatt ttcagcgtat ccatttttgg
     2101 ctccggtgat cagcatagtg gtaaattcct ttgggaatga atgggcaaaa ctgtgtaatt
     2161 ttgtactaaa aagacagtag gtaaccaaaa agccactggc atttccacca ctggcttcgg
     2221 ctgcggaggg cgagttccaa tggcttgccc tgcggggatg ttccaggtga ttttggttgc
     2281 ggattattgt attgacagcg cgcttttttc aatcatgatt tttgatacct tctccaattt
     2341 agttggtgcc caattattga gaaacaaaca agaattattg gtttaataca ggtttacatt
     2401 tccaagagcc ttaaggtatt cggcttttcc gttttagatt ttccgattat tcttaggtct
     2461 tttgccaagg ttaacaaatt cccgggcagg ggcttctaag agtaaggtcg aaaaaaatgg
     2521 cgcagttgcc atttaagcag atttgccata agtttcaata catcccgatc gatcagaaga
     2581 ccattcttac cgaataaatt ggagaaaatt catttttggg ccttcttcca atcgggtggg
     2641 tgatttcggt cgattatcta tctgcccatg ttttctctgc cagtaggcgg gggttggccc
     2701 gggcccattg ggtgccttcc gtccgtggca ggatctcaat gaggcagtgg ctctttcatg
     2761 gttccgggta ggaaaattct tgggttggcc gggtctgacc aagggttcag ttttccgcga
     2821 cgggatcggc ggcaggtgcc ttgcggaaat caagggaccg cttcagaatc cgagcttttt
     2881 cccgccgatt ctgtcctgcc atggacggga agcaccccct gggcctttga gggagaactg
     2941 tttgggtatt cttacccatt cgattgggag aaggcccatt aggaacatga tcgaaaattc
     3001 gttgcagaat ttgcgtagcc gatttgaaat ggaggggaaa ctccgccggg attataccta
     3061 tgtcaaggat tgcgtcagag cattgattgg ggcgcttgcg gcagaaaacg aaaaattgga
     3121 aaatccagct tacttagcga ggtcttatca ataatttctg caccaagtcc ctagaacacc
     3181 gccgtcttgt ctttttcagg tcttacccat ctgcgaaatc tgcgtagtct acggattcag
     3241 ggttttcgtt tacgtccggg aataccgctc ctcgatccga agcagttcgt tccgcgtggc
     3301 atcgtccaag gtgggctgtc gaggcgccgc gaggagggca tccacacggt cttcggcccg
     3361 agtgatcagg ttgctccgcg catccgcgag ggaacgctga aggcggaccc cgagccgggg
     3421 ctcgaagata tttccccgaa aatgttggaa ggtgtggggc gagctcataa actcgcccga
     3481 gattcctacc ctgcggatct cctccagggc cagggtctct tcgtccaccc ggaacccggc
     3541 aaggtacttt ctaatcatcc ggacgatttc atcgtcgatg accgcctgga cgggggagac
     3601 cgtcttttct gattcgaagg agccaatccc ccacacaacg ctcactctcg cctgggcatg
     3661 cgtcaaggca gccagggtct tttccatggc gttctgcgcc tcatatccca gggagtcgga
     3721 gcagatccag gaggcggagg ggacccggta gaaccgggcg aggtccgcgc cggctgcggc
     3781 gaagagacag ttttcggggc tcccggtaac ggcaaagccg aaacgcatgt ccatcacgtg
     3841 ggcaaagccc aggttgaaaa agcacggccg gccgggctcc agaacctggt tgatgatgat
     3901 cccgctcagg atctccgccg tgcccaccac cgcagtcccg gccagggtca ccgggctcgt
     3961 ggcgcccgcc atgggctcgc cgttgaccgt gcaggggatc ccgtggcccg cggtcgcctt
     4021 gaaaaccccg agggccaggt tcgtccaccg cagcggtccg tgggcggtga agccgatgct
     4081 gaagagcggg ttttctcgga gtgatttccc tcccgcaaga acccgggcca tttcgatcat
     4141 ggcctcgccc ccccgggggg taaaagagag ggggcggaga tgttttccgg tattctcggc
     4201 catgatccgg aaacccacga aatcacggtg ggggggcggg gtatcctcgg tggccgtgcc
     4261 cacgatgcat tccacctcgg cgaggttctc gatcacccag gcgaagtcag ccaggtcctt
     4321 ccgttcgatg ggacggaatc ctttccggtc gagataaaag agggccgccg cggtccaaaa
     4381 gcccgattct ccctccgggg agacctccct ccggttgcct ttccggtccg aaatcgggaa
     4441 cctatccgga gccagggaga ggtattcctt taccatggcc ccaggaaacc gcacgacctg
     4501 ggccccgacg ccgggcttgg cgccggaggc cagaagcttt ttcacaatct cctcgtcttc
     4561 gacccgcaca cccgggtcct ccaggagtgc tttggctgcc ccgtcgattc tttcgatgtc
     4621 cgacatactc aacatagaag atactccgag atcataatgt agggcggccc gatgtggccg
     4681 cccacaaaat ggacaaccat tacgcgcttc gcgtgattgc cgctacgatt tagttgcgtc
     4741 tctgcagcat cgcatttgcc gaaactgcaa agggtgaagc cgaggagatt tctcggtccc
     4801 cgggcctact cttcaattcc caggtacgcc tttttgacca caggatcttc agcgatcttt
     4861 tttccttctc cttgggcgac catccggccg gtctccagga cataggcgta atcggcgatg
     4921 atcagggcct tctgggcgtt ctgttcaacc aggaggatcg tgagcccatc ccggtgaagg
     4981 gtctcgatca tcttaaaaat ccccaggacc atgagggggg cgaggcccag cgagggttcg
     5041 tccagaagca gggtccgcgg ttcggacatg aggcccctcg cgatggccaa catctgctgt
     5101 tcccctccgg aaagagtccc tcccgcttgg acctgccgct cttccagaat aggaaaggtg
     5161 cggtagacct tcttcaagtt ctcctcgatc cgcttgcggt cgtggatcat gtaggcccca
     5221 aggaggaggt tttccttcac ggtgatggag gagaagatct tgcgtccctc gggaacctgg
     5281 acgacgccca ggcgggcgat caaatggggt gagatgacga ggctccgccc ttcgaagata
     5341 atctctcccc ccgaaagcgg gaccatcccg ctgatagcgt taatggtggt cgacttcccg
     5401 gcgccgttgg cccccacgat gctgatcatc ttcccgtccg ggatggccag ggacacccgg
     5461 tcgagggcgg taatcccgcc gtagcgtacg gtgatttctt tgacttcaat catgcttcac
     5521 tttagcgcac ttgaggaatt caaggcactt gaggcacttc tctatacctc gtcgccgagg
     5581 tacgcctgaa ggacctgctc attcttcttg atttcctcgg gccgtccttc ggcgatcttt
     5641 ttgccgaagt cgaggacatg gagggtggtg cagatcttca tcaccagctc catgtggtgc
     5701 tcaactacga tcatggacag cttcttctcc tcccgaatct tgaggagggt gcccatgagc
     5761 tcccggatct ctttcgggtt gagccctgcc cccggctcgt cgagaagaag cagcctgggc
     5821 gaggccatca gggccctggc cagctccagc ctcctctgca ggccgtaggg gaggttgtaa
     5881 gggaagtaag cggcgtattt ttccatgccg aagtaggaga ggtagctctt ggccaggtcc
     5941 gcggcttcct tctcctcccg ccggacccgg gggaggcgga acatctcttc ccagacggtg
     6001 taatgggtct tcactccata agccaccgcg aggttgtcca ggacattgag gttccgaaag
     6061 agccggatgt tctggaaggt ccgggtgatg cccagagcgg taatcacatg ggcgggcctc
     6121 cgggtggtct ccttgccgta gaaccagatc tcaccggtgt ccaccgggta gactccggtg
     6181 atgaggttga agaccgtcgt cttccccgct ccgttggggc cgatgatccc ggtgatctcg
     6241 ttctcccgct ggagcatgga gaagttggcc acggccgcca ccccaccgaa gttcttcgaa
     6301 acctcggtga ctttcaggac ctccgcggtc atcccgcctt cctcctccag aagaggagat
     6361 tgcccggggt cagctcccag ttcccgaaga tcccctggga ccggaagatg gtcaccagga
     6421 ggaccgcaaa ggccaggagc acgtccctcc atacgtccac gaaccggaag aactcgccga
     6481 aggagtaata aacaatggag gtgacgatgg cgccggtgat actgttgacg ccgcccacga
     6541 aggtgatgat gatcagcttg gccgattcga gccagttgaa gaggttcggt tcgatgaaca
     6601 tgatcacgaa ggcataaagg gccccgccgt aggcggtgat cgccgaactc aggatgaaga
     6661 cggtcatctt gtgccggtag acggggatgc ccataagggt ggccgccagc tcatcatccc
     6721 ggatggcgat gcagttccgc ccgaaacggg accgcttgag gcaggcggtg atcagggtga
     6781 tgaccacgat ggagaggatg atcgtgggca ggccgatgag cttggggatt ccggccacgc
     6841 caagcgcccc cccggtgagc tgggctaggt ggagcaaggc cgcccggacc atctcgccga
     6901 agccgaaggt gaccagaagg aagtaatccc ggtggaggcg gagtgtgggg aggccgatca
     6961 caaaccccac caggaccccc atccccaccg ccaaagcgag acaagcggga aagggcaggt
     7021 tgaacttgag aaaagcgatg gcggctgtgt aggcgccgat ggagagaaac cccccctgcc
     7081 ccagggagaa gagaccggtg aggccggtca tgaggtaggc gcccaggacc aggatcgtgt
     7141 ggagacaggt aatttcgagg accttgagat agaattccat ataaaagaat cagaagtcag
     7201 gagccagaag ccaggagaaa aaatccttat tctgactcct ggattctgaa ttctaggcct
     7261 ttataatgta cctcttcccc gcgatgccct cggggcggat catgaggagc aggaaaatgg
     7321 ccccataaac ggcgatctgg gtgagggagg acccgatctc cgcgatgagg aggctctccg
     7381 caacgcccag gaggagcccg gcgatcaccc cgcctcccag gcttcccata cccccgatga
     7441 tggaggcgat gatccctttg agaatcatgc ttccgataaa ggggtccaca ttgtaggtca
     7501 tccccaggag atatcccgtg atcccgccga ggaccccggc catgaaaaag gccgaggaga
     7561 taatatgatc tacattgatt cccatgaggc tcggggtgcg gatgtccgtc gaggctgccc
     7621 ttaaggcgat gccgatgcgg gtgcgccgga tgacccaggt gagcagaagg aggaccaccg
     7681 cggtaatgga gagcatcatg aggtacgtct tcgagactgt caggttcccc aggtggaagg
     7741 ccgtctgctt catgaattcg ggatagggga agtagtcgtc ccccagggta gcggagaaga
     7801 actgctggac cagcatggcg gcggtgatcg aattgaccag gaagagggtc tggggggcgc
     7861 ccttgaggcg gatgggccgg aagatgagtt tttcaatcag gacgccgatg aatccccccg
     7921 ccaccgtagt cagaatcagg gtcacgatga aattggagta gacctggcgg gcgagaaagt
     7981 agcccaggta cgcgcagatg gccacgaggc cgctgtggga gaagttcgag aacttgagga
     8041 tgttgaaaat caacccgaac cccacggcct cgagggcgta gaccccgccc agggagacac
     8101 cgttgaagag ctgctggagc aggacgctga aatccaccag agagcctcta gaaaaaaaac
     8161 aggattcagg tttcaggatt cgggaatcag gtccccggtc tcaggataca ggattcagga
     8221 ctcaggtccc cgacttccag ctcctttctc ctgactcctg tcttctgttt gcccggcggg
     8281 attccgtctt ttcggaaagg ccgatcagat atccttcggg tagtacttca acaggaccgt
     8341 cttgatcttg tcgccctcga acttgaggat cgacatcttc aagcccacgg ggcggtgggt
     8401 tttggggtcg atgttgatgg tcccgatcaa tccttcgtac ttggtcgtcc ccgccatggt
     8461 gtcgcggatg atcttcgggt cggtggactt gcatctcttg atagcttcga tgatgaagcc
     8521 cacgtcatcc cacccggaga aggagtggag ccggggcagt tcgccgtact ccttgttgta
     8581 cttgtcgatg aagggcttca ggctcgggtc atcccgggag atgttgaggg ggaagtaagc
     8641 cccgtcggct gccgcgccag ccaccttgtc catgggctcg taccaagagt tgttcccgag
     8701 catggtcgtc ttgatcgcca gttcgcggac ctgcttggcc gcgttggcgt tctccggcac
     8761 gtagttggga atgtagagac cttccggccc cagggccttc accttggtga gctgggcctt
     8821 gaagtcggcg tcgccggcct ggaactcgaa gttccccagc aacttcttcc cccgcttctt
     8881 gacgtagtac tcgaacccct tggccagcat gaaggagtag gagttcgacg gcgtgaagag
     8941 ctgggcgaag gtgttcagct tcaactcatc gatggcatac cccgcgatca tgtaggactg
     9001 ctggtagctc gatggctgga ggaggaagca gtacgggttg acgcggccgg ggttttcggg
     9061 gtcttccatc ttgaagtccg gggtggtcac gcgttcgtcc agggcccggg cgaccacggg
     9121 aaccttcacc tgctcggcca cgggcgaaac cgccaggccg atgttggaga tgagggaccc
     9181 gtacaccgcg gaaactttgt cttccttggc caaccgggag taaaccttca caccttccgt
     9241 ggggctcatc ttgcagtcgt aggcgatgag ctcgatcttt tttccgagaa gtccgccggc
     9301 ggcgttggct tccttgacca taagcttagc gcccttgaga gcgtcattcc cccactgggc
     9361 ggagccggcg ctcaggtcgg cgtacattcc gatcttgatc gtctcttgag ccgcagaagg
     9421 catggcgcac acaaggacca tcgccaaagg aaaaaggacg ctgaacagac tcttcttcat
     9481 aaaaccccct ttatatccgt tctccttaaa tgttgcgtcg acaggaatac aggaatggaa
     9541 ttctttagaa atttattata ttttcctcgg ttttcccttg tcaagcggaa agcggccctt
     9601 gcggaccttg cccaccccca gtttttaaaa aagagtcctt cgaaaataag atctctggaa
     9661 aggagggggg atctcatggg gttcagggtg gaggaatttg gatcatcagg ctaagcaccc
     9721 gccgagcctg atgatccaaa ttcctccacc ctgaacccca acaagcatga aaaactgggg
     9781 gtgggcaaga aggcccactt cattgacacg gttggaaatc catttttata atcatattga
     9841 gggaaaatat cgaaagggga gtcgtcaggt ggctgggcca aagttgaaat atctgggttt
     9901 aacggaaaga gaccttgatt ttctggtgga gactgtctcc cccgaagccc gggataaagg
     9961 ccggctcaag caagtcatcc gggaagacag agatatcaga aattcctacg taggggatga
    10021 aaaggttttt caaagattaa tggacgaaga agagagcttt gtaaaaatct ctcccatcct
    10081 cttctttgag gtcctgctgc ggaaggcggc gcaggatttg gaaaaggccc agtacacggt
    10141 cgaaaagtcc agaaccatga agatcccgat cttcgatact caagaggtgg ttggacttct
    10201 aaacagggaa gacctgctat tttatttggc ggatatgctt tcgtccttca cccgagtgga
    10261 gagttatacc attcctgtca ggatcaggga gggaatctgg gaaagggtcc ggtttaacga
    10321 cctggatatt ctcagcctga tgcggttctc ccagttcgtg gataatgaac accggcttgg
    10381 cttctacaaa aggattgctg atatctgtct cttcatcatg ggaattttcc ccgactatgc
    10441 cgaacgcgat tatcggtatc ctttttccgg ccaggtgcgc ccgcatgttc gcgggaagct
    10501 gaggatcagc ccggcggact atgcggaaga ggggcagaaa ttctataaac tcgctgcgca
    10561 acatccgtcc gccagagaaa tgcatttgga agagattttc tgggcccttc accgcaattt
    10621 tcaaaaggcc aaaaagccgc tgaactttat cgccgactac tacctccaat accggcggga
    10681 tagatttttt ggatgacccg agggtgaagg ttcagtccga tgccgccatt cagatcgatg
    10741 ggtggtgtga ggccaaagtt tgccgttttt ttatgcccca agcttccgtt ggccggagtc
    10801 ctttatttgg tcgttttgat cccggacttc tggaggtagt ccttaaacca ctcatattcc
    10861 ttccttacct ttttctccag cccttcatgg tcgaggtaat cgtaggggac atcgaagctt
    10921 gttaggactt tctggaagga aggctccagg gtgactttgc ggatcgcctc gcccagcttc
    10981 tggctgatgg acgggggcag gcccttgggg gcaatcacat cgatccccgc tggggggaca
    11041 tcggggcagc cgatctccct cagggtcggt acgtcgggaa aagaggggtt gcgcttctcg
    11101 gcgttgacct ggacgagaag gcggaagact ccctgccgca cgtagacgat ctggctggac
    11161 ccggtggaga agtccgcgtg accgcccatg agcatggtgc tggcgtcagt gccccctttc
    11221 gtcgggacat ggcgaaaggt gagacccttg cattgagcca ggagctcggc gctgatctgc
    11281 tgacgggtgt acatgcctgt cgaagtgtag gacattccgg ggtgggcctt ggcgtgggcg
    11341 ataaactcat ccagggtctt ccacggagag tcgctgcgca cgacaaagga cccgaagtag
    11401 gtggcgtact gagccaggaa ggtaaagtcc ttgagcggat cgtaggcgag tttttggagg
    11461 agggggttgg cggtcagggc tccttcgctg atcactccca gggtgtaccc atcgggcttc
    11521 ttgctcgcca ccaggccggc gcataccgtg gttcccccgc ccggtttgtt ctccacgacc
    11581 acagggaccc cccacatctt ctccaggccg ctggccaggg accgggagga gacatccgtg
    11641 ctggccccgg ggccgtagcc acagaagatg gtgatcggct tgtccgggaa ctcgggaaag
    11701 gccggagaag tcaaaagcag aaaggccatg cccaaaatga ttgctgaggt cttccgatag
    11761 ttctttctcg ttcccatccc ctcaaaccct cctttttcct ttttggaatc gtctcccgac
    11821 gcaagagcct tataagggta acggctaaga agaaaaggca agaatggtcc gattcaaggc
    11881 gaatcataat ggtttggcgc ttgatagtca aggatacaag aattttcaat tgatgcttat
    11941 cgaaaaaagt ttcaggactg caatcttggg atttcaattt cctataataa accggacatc
    12001 ggttcggtct ccttaggatc cccgatttga aggaccccgg cggaggttgg acatgcctcg
    12061 gaacgaggaa agggcttatg tccgtaagag caatgcttgc tcttccagca tcaaggaatt
    12121 gggccagccg atagatagga ccgcattagg gggccaggac tatttttcta tctaatgatc
    12181 tggcagttag cccaatcaga agggttctga ttgaaatctg gcgcaataag tctaaggctg
    12241 ctcaaatcca gcttcctttt tcttgtccca caatgccaca gactgtttct ttattcccca
    12301 ccacgatctc cttgccattt atcttcaatc tttaaaatct gttgaaatat aaagggttcc
    12361 gttttctgcc gagttggcag gtcttttgct ctctatgaaa aggagaagat agaccaacaa
    12421 aaaaagtcag ccggggggag ttaatggcta agtggggata aggaggtgca tagctatcga
    12481 atatctcgtt gtagtcaaag aagagactaa cctccttttc gattgatgcg caacgaaaat
    12541 cgggggtatg aaccgaatta tcccctcttt ttctatgttc cacggggaga agtcagagct
    12601 cccctttgat atttccccgg aggtcccctt ttcttgaacc cgccggattg acagaatctt
    12661 gggtaaaaaa gggaaaagaa gaatgaaaac aatatttgcc tcagtggttg tactggcgct
    12721 gctaaccttt cccctctttg ccatgacgaa agggtaggaa gaatctttga aacagtcttg
    12781 gggttgctcc ccatcgccgt aagatacaga gtcaatcgct gagagtgact caaagaaagg
    12841 agtggaagag atgaacaaaa tgtgggctgt ggtagcagta gttcttgttt tgggccttgg
    12901 ctttgcgact ggttctcaag ccaaacctat gagccaaagc gggctaagaa tttacgacac
    12961 aacaaaattg atagggagta cggtaaaatc ccctgatgga gtgacgctgg gccaaatctt
    13021 tgatctggtg gttgattccc agggccatgt ggattttgca atcgttagcc agccgggttt
    13081 tgaagaattc ccaggtcggc tggtagcggt tccttttagt tccctgatga tttcagaggg
    13141 gaagtctcag caagcccaag tggttctcca cgctgaccaa gagaaatttt atgaagcccc
    13201 aagttggggt gacaaaaatc tcgctgaccg gcaacaggcc gcttcgctgg acagatattt
    13261 cggagttagt ccctattggc cagaaaaaac cggacaggca cccagacagt aaaacggtga
    13321 aacctatcta tcttttatta aaaggcgaga gagtcgtgct ctcgcctttt tgcattcgat
    13381 ccacaaatga attttagcat gccagggagt tgactggtgt gagcactaaa aatcctccct
    13441 tgggattatt ttgtcagatt tcatccctag gcaaaggtaa cgaataaata tgaatggctc
    13501 cttctgaccc gccttgtccg tgggatgttt ttttggaatt gtagcaatcc tttgccgcga
    13561 agcgaccggt tgaaaaatca tccgtccatg ggattcagcg gctggctata tcccagccgg
    13621 ccccttcctt tttccagtaa gggatttggc catagtatcg gtaaatttct tcccattggt
    13681 tcgggcgcat tttcattcct ttcgatcggt cgtaaggcgg tgcggcgaga agtctttgcc
    13741 ggtcaaaatt caggattacc gtcttttctg gagactttgc ctccgaaatg gagaaagcgc
    13801 taaaaggaac cagcacatat cttgcaacat caaagtcttc aatggcacct tgatagagaa
    13861 ccacgaaggg aaggcccatg gggtccaggg tgatgtcact aatcattccc aagtcctccc
    13921 cgacgggatt ctttacctgc attccgatta agtcggtcag ggtggatggc aaaccttcct
    13981 tttcggccag agaaccttgc gaggtcgaat aagcttcaat tccaaggctc aagaatgaga
    14041 ccaataccga tatgattaca attttcttcg acatctccgc cttcttatcg ttttttaaat
    14101 cgaaatattt tggtggaatc tccagaattt catttgtttt ttaaacccag gctttatcgg
    14161 cctcacaatg attttgatgc cctctatcga gaatggttcc gacaggaaat tccgatccgg
    14221 gtcttaaaaa catcttcagg tccctaaatc acaggatgcg cccctcatct ttggcagatg
    14281 caaaaaaaac ggaggaggct tacaataatt caggatgcat gggtagttgg cgggatggcc
    14341 acaaatgata aagatcgact tgaatcccat tctagtagca ctggggccca tccgggtgac
    14401 ctggtacgga atgatgtatg tccttgggtt tattctctcc tatcttttgg tccgatctca
    14461 ggttcagaaa aaagattttg gcatttcctt aaaggaggta gaaggtctct tttttgatct
    14521 ctttctggga ttgatcatag gggcccgcct gggctatgtg ctcttttatg acctccaggc
    14581 ttacctctcc aatccccttg aaatttttgc tgtatggcat ggcgggatgt ctttccacgg
    14641 gggcctgatc ggagtcctca tcgccgccgt tcttttttgc aggaaacaga aaaagtcatt
    14701 ttggaaactg agcgaccttt tcgtcgtcac tgcccc
//
LOCUS       DASPRR010000218         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1988065, whole genome shotgun sequence.
ACCESSION   DASPRR010000218 DASPRR010000000
VERSION     DASPRR010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988065"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /gene="trpD"
                     /locus_tag="VEL68_08445"
     CDS             <1..182
                     /gene="trpD"
                     /locus_tag="VEL68_08445"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXZ36032.1"
                     /translation="ILAGKDGACRKTVLLNAALAIVAGEKAETIQEGIRVAEDCLDSG
                     VAGKKLEALVELTHR"
     gene            376..1155
                     /gene="trpC"
                     /locus_tag="VEL68_08450"
     CDS             376..1155
                     /gene="trpC"
                     /locus_tag="VEL68_08450"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXZ36033.1"
                     /translation="MFLQKILEHKKQEMSERKALLPLSELKRRLEEEPAPLEFERAIR
                     SRHEALIAEVKRSSPSRGRIREDFEPAAIARLYAQSGAAAISVLTEQRFFEGHDSFLR
                     QVRKSVSLPLLRKDFIIDPYQIYETRFLGADAILFIARALDSGLLGEFLRLSSELGLS
                     SLVEIHDENDLEKAVSAGARMIGINNRDLSTFSTDLETSIRLAPLLPKEVTVISESGI
                     RSRRDIERLRAAGVHGFLVGEALMTAGDIAGKIKELLGEEG"
     gene            1159..1848
                     /locus_tag="VEL68_08455"
     CDS             1159..1848
                     /locus_tag="VEL68_08455"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXZ36034.1"
                     /translation="MTEVKICGITNREDALTAIHAGADALGFIFYPASPRFVKPDQAR
                     EITQRLPGNICRVGVFVDQDPEEVRGIMRFCSLDLIQLHGKESSDYCALFSPSVLIKA
                     VSPGKEEDLAVLGDYPVKAILMDASGPGQPGGTGRTCDWSLARKAGERYRLILAGGLH
                     PGNILLALEMVSPQAVDVGSGVEEQPGKKDPEKVRELFAAVKEFNNLRRSDPPSSIFH
                     KEGECQYKQDK"
BASE COUNT          467 a          547 c          601 g          422 t
ORIGIN      
        1 cgatcctcgc cgggaaagac ggggcctgcc ggaaaaccgt tcttctgaat gccgccctgg
       61 ccatcgtggc cggggagaag gctgagacta ttcaggaagg gatccgggtt gcagaggatt
      121 gtctcgacag cggcgttgcc ggaaaaaagc tcgaggccct cgtcgagctt acccatcgtt
      181 gatgatttcg taagaagtcc cttgcgccgt caccccggtg aaggccgggg tccagatgtc
      241 gtcccgacga aagtcgggaa ccatctcaaa gactgggtcc cggttttcac cggtaagcct
      301 ggattccggc tttcgccgga atgacagatt ctttgaattt ccacttttta cagaatcatc
      361 acagttgagg caagcatgtt tctgcaaaaa atactggagc ataagaaaca ggaaatgtcc
      421 gagcgcaagg ccctcctccc tctttccgag ctaaagcgac gcctggagga ggagccggcc
      481 cctcttgaat ttgagagggc gatccgctcc cgccacgagg ccctcatagc cgaagtgaag
      541 agaagctccc cctccagggg gagaatccgg gaggactttg agccggcggc catcgcccgg
      601 ctgtatgcgc aaagcggcgc ggctgccatt tccgtattga ccgagcagcg cttcttcgag
      661 ggccatgatt cttttctgcg tcaggtccgc aaaagcgtgt cactcccgct tctgcgcaag
      721 gacttcatca tcgaccccta ccagatatat gagacccggt ttctgggggc ggatgccatc
      781 ctgtttatcg cccgggcgct ggatagcggc ctgctgggag agttcctccg cctatcatcg
      841 gaattggggc tttcttctct ggtcgaaatc cacgatgaaa acgatttaga gaaggccgtc
      901 tccgcagggg cgcggatgat tgggatcaac aaccgcgacc tctccacctt ttccaccgac
      961 ctggagacaa gcatccgtct ggcccccctt cttcccaagg aagttacggt gataagcgaa
     1021 agcggcatcc ggtcccgccg cgacatcgaa aggttaaggg cagccggagt ccatgggttc
     1081 ttggtgggag aggccctcat gaccgcaggg gacattgccg gaaaaatcaa agaacttcta
     1141 ggggaggagg gatagaagat gaccgaggtc aagatttgcg ggatcaccaa tcgtgaggat
     1201 gccttgactg ccatccatgc gggtgccgat gccctcggat tcatttttta ccccgccagt
     1261 ccccgcttcg tgaagccgga ccaggccagg gaaatcaccc aacggctgcc cggtaacatt
     1321 tgccgggtgg gagtcttcgt cgatcaggac ccagaagaag tcagggggat catgcgtttc
     1381 tgcagtctcg acctcatcca gctccacggg aaggaatcct cggactactg tgcccttttt
     1441 tccccatcgg ttctgatcaa agccgtttcc cccggcaagg aagaggactt ggccgttctg
     1501 ggcgattatc cggttaaggc catcctgatg gatgcctccg gtccagggca gccgggcggg
     1561 acaggcagga cctgcgattg gagtctggcc agaaaggcag gagaaaggta tagactcatc
     1621 ctggccgggg ggcttcaccc cggcaatatc cttttagcac tcgaaatggt ttctccccaa
     1681 gccgttgacg tgggaagcgg ggtggaggag caaccaggaa aaaaggaccc ggaaaaagtg
     1741 agagagctgt ttgctgcggt gaaggagttc aacaatctac ggagatcgga ccctcctagc
     1801 tccatttttc ataaggaggg ggaatgccaa tacaaacagg ataaatagcc tgcgaggagc
     1861 caagcgacga agcgatccca atcaagattc agattgaccc gccctggcgg gctcgcaatg
     1921 acctgacatt gtttcattcg ttttttatta gaaaatgaac cggaggtagg agaatgggaa
     1981 gaaattcttt gcccgataag agagggcatt ttggaatctt cgggggaagg tatgtag
//
LOCUS       DASPRR010000219         9161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1664374, whole genome shotgun sequence.
ACCESSION   DASPRR010000219 DASPRR010000000
VERSION     DASPRR010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664374"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VEL68_08460"
     CDS             complement(<1..635)
                     /locus_tag="VEL68_08460"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXZ36035.1"
                     /translation="MALVIEGGLDIPIPRMVPIRQKFLGPEIKDIGEEIKRLFSQDGR
                     IKKIKKGSRIAITAGSRGINSIDRIILALARELQALGTKPFIVPAMGSHGGATAEGQR
                     KVLAEYKITEETMGVPIESSMEVEKVGQLENGVPVYLDRIALKSDGIVVVNRVKPHTA
                     FKADYESGLLKMLAIGLGKHKGATAFHTCGVDMFGELLPQLGKVVLDNAPVL"
     gene            933..2168
                     /locus_tag="VEL68_08465"
     CDS             933..2168
                     /locus_tag="VEL68_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36036.1"
                     /translation="MFRKKALWAVVSAFLFLLFAVTPSGAANRDFSKINTVKMGILAP
                     VQMPVGHGIMNAAKLAADEINAAGGIHGKKIELVFGDTESKPEKGVTAMKKLVLEDKV
                     DVLVGEYSSGVSLAIQPFLSGYKIVFITTGCASIALTDNVKKDYAKNKYFFRSMINSD
                     IRQEKWAFKFLKEFVNGSLGYKRFAILAENAKWTEDYAPNLKKDLEGAGLEVPFMERF
                     DVDIKDFSPIFSKIKSLNVQWIAQIVSHAASIPMVKAWADNKPAPMGCVNVTSQDAKF
                     WEMTGGACLGEATYNFIARAPLTDRTIPFWDSYEKKFGNKPMYPSGFTYDAVYMLADV
                     IKQKKSVKSDDLVTGLENISYKGVIHPETGYDKQSHDILEGRYVMPVVQWQAGGKQVV
                     FWPDQFKVAPYVKPEWWKQ"
     gene            2309..3172
                     /locus_tag="VEL68_08470"
     CDS             2309..3172
                     /locus_tag="VEL68_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36037.1"
                     /translation="MTALVSIFVLGTIWGAVYSLIAVGFTLIFGVAGIVNLSHGAFYM
                     LGAYLAYTFMTLLNINVPLSALMAVAAVALIGMAIDYLGIRPLRERHAYVLILTLALA
                     LFFQELMYGIYGPYGKPVKSFVVGEIVLGGVYISYQKLITLFVAAAFVILLWLFIKKT
                     KTGKSISAVAQNKDAAILMGIQYEKAYRMTMGISAGLAAIAGVFISPILEAVPTMWSF
                     PLFKAFAIVIIGGLGSLEGAIIASLLLGYSETLVSFLFSANYADMVYLIAIILVLVLR
                     PTGILSKGRRA"
     gene            3244..4215
                     /locus_tag="VEL68_08475"
     CDS             3244..4215
                     /locus_tag="VEL68_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36038.1"
                     /translation="MEGYEELFGPRRFYRRLLGLGIFSAIVLLAPLVVEDPYYLELLF
                     LCHYYVILACSWDLLSGFTGNINFGHAFFIAGAGYTGALLNLNLGWQYWLTLPAGGII
                     AALFGLLVGVVTLRLKGPYFAAVTFCFGALLYKWFMVSSKSLGGEEGLSGVASLSDSP
                     LGNYYFSGIFMIAVVLGLYFLSKSSFGLILRSIGENETASEGSGINTIYFKVVAFMIS
                     AAIAGVAGAMYAHAQMHVGPEMAHDSLSALVLMMAVVGGMGSIIGPIMGAYLLTMVNE
                     SLRFMGELRLLIYSGAVVAVILFAPKGLLEILSQGFRRVTGDRQKAV"
     gene            4237..4974
                     /locus_tag="VEL68_08480"
     CDS             4237..4974
                     /locus_tag="VEL68_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36039.1"
                     /translation="MDAVLEIQGMSKAFGGLQAVKNFDLQVRKGDLIGIIGPNGAGKT
                     TVFNMITGFIRPDSGEVRFRGENITRLKPFEIVNRGLCRTFQLVDLFKEMTALENVMI
                     PCFSHRVRQRRAQEEKPWKTAMKFLEEMGLAKRFNEYAKNLPFGELRLLDIARAMATQ
                     PDILLLDEPFSGLDVEDASVLSAAIKQMHQKGQTMIIIEHRLRDLLKLVDRVVAIVFG
                     EKIAEGNPQEIMNNPKVVNAYLGERRD"
     gene            4991..5686
                     /locus_tag="VEL68_08485"
     CDS             4991..5686
                     /locus_tag="VEL68_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36040.1"
                     /translation="MKAIKVFYGKAIALNGLTLSIDEKEMVGVVGPNGAGKSTLLRAI
                     SALIPSEGDILFQGDRIDHHPPHEIVRKGIIHCPERRQLFVDFTVAENLEMGAFLSKD
                     KEKIREDLSHVFHLFPVLEERRNQLSGTLSGGEQQMLAIGRSLMSSPRLLMLDEPSMG
                     LSPLVKNLLVEGIKTIWKSGLTVLIVEQDASLALNLVERVYILEHGKVGLEGKSRDLM
                     NNEEVKRVYFQLG"
     gene            5868..7553
                     /locus_tag="VEL68_08490"
     CDS             5868..7553
                     /locus_tag="VEL68_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ36041.1"
                     /translation="MTSFYENKPWLKQYSPWVPPELEIPEENLVQILKRSVTSAGGTA
                     IYYFHRSISYTDVDRWSDSLARALQDLGLKPGNRVIFLMQNIPQFIIAQYAVWKAGGI
                     VVPLNPMYKEKELDYFFQDSGARALIAISSAYESSARNIVRERKMEIVITTSGLEFLS
                     PDEPVPRMLQEEKYTRFPEAMDFMDLLKKYGEEVPHPTELPNHQSAYFTYTSGTTGPP
                     KGAMNTHRNIAFNAKFYTTFLRLASPDVIFGVAPLFHVTGQVAHMAAAAYAGIPLILS
                     FRFDPEEALKLIERWKATMTLGATTVYISLMNHPNFRKAAFSSMTKVYTGGAPVPPAV
                     VENFEKSCGAYIHNAYGLTETNSPSHFTPWGMRAPVDPETGALSVGIPVCNSLSKIMD
                     LETGKEELPPGEIGEIVVRGPMVVPGYWNKPEESAHAIRDGWLYTGDVGKMDERGWFY
                     VVDRKKDLINVSGFKVWPRDVEDVLYQHPAVREVAVIGVPDPYRGETVKAFVALKEEF
                     KDRVTPVELIDFCKARMAAYKYPRVVEITPEIPKTTTGKFLRRILREKERDKT"
     gene            7593..8432
                     /locus_tag="VEL68_08495"
     CDS             7593..8432
                     /locus_tag="VEL68_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HXZ36042.1"
                     /translation="MAKQYSRLEVRKRLEAEIKKKAPIVAAGAGIGLSAKFAEAGGSD
                     LIIIYNSGLYRMDGLPSIAGYMPFGDANAMVKEMGLRHVLPIVQKAPVIAGICAYDLT
                     RDMEVFLKELLAVGFSGVINFPTVGRIDGDFRQTMEDMGMNYRGEAEVMGLAGSLGLF
                     TMAYVFNAEESRMMARKGIDSVVAHMKTTTGGTVGSKRPMGLEEAAKRCSAIFKAALS
                     VKRNVICLAHGGPIEGPAATEYIYRHTQAVGFVGASSIERIPVETAIQNTTEMFKTQR
                     LKR"
     gene            8644..>9161
                     /locus_tag="VEL68_08500"
     CDS             8644..>9161
                     /locus_tag="VEL68_08500"
                     /inference="COORDINATES: protein motif:HMM:NF018498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HXZ36043.1"
                     /translation="MDKSILIIGTFDTKAEELNFVKDQILAIGEKALTLDVGALQPSP
                     DYVDISNEKVAAAGGRPIYELVSRRDRGQAVAVMAQGAAKITRELFDRGEISGVISLG
                     GGSGTVIGTRAMRTLPLGIPKVMVSTMASGNVRLYVGISDICMIHSVVDISGLNRVTK
                     KILGNAAAAVCG"
BASE COUNT         2034 a         2496 c         2597 g         2034 t
ORIGIN      
        1 agaaccggcg cgttgtccag gaccaccttt cctagctggg gcagcaactc gccaaacatg
       61 tccaccccgc aggtgtggaa agccgtggcc cccttgtgct tccccaagcc gatggccagc
      121 atttttagaa gcccgctctc ataatcagct ttgaaggcgg tgtggggttt cacccggttg
      181 accaccacga tcccgtcgct tttcagggcg atcctatcca gatagaccgg cactccgttt
      241 tccagctgcc ctaccttctc cacttccatg gaggattcaa tgggcacgcc catggtctct
      301 tcggtgatct tgtattcagc caaaaccttg cgctgccctt ccgcggtggc ccctccatgg
      361 cttcccatag cgggaacgat gaaaggtttg gtccccaggg cttgaagctc tcttgccagg
      421 gccaaaataa tcctgtctat tgagttgatc ccccggcttc ccgcagtaat ggcgatgcgg
      481 ctgcctttct tgatcttttt tatccggcca tcctgggaga agaggcgctt gatttcctct
      541 ccgatatcct tgatttccgg gccgagaaac ttctgccgaa tgggaaccat tcgggggata
      601 ggaatatcca aaccgccttc aataactaga gccatagatg ccctccgtat ttccatttca
      661 ttttaaggga tttgattcta tctacctttt atcgcacgaa gggcaaggtg tcaatactaa
      721 ccaggaaaac accaggaaaa cccttgacag aaggctttgc ttatgattat ctaggccggt
      781 atgccaaaaa aggtatacct cgaatggccc ggcctatcta tcctcgagga ataccagcca
      841 tcgggtcaaa aggacgccag aaaaaagccg gtttcagaga aaccccaaat aggccggaag
      901 aaaaaaacgc tctggagaaa ggagacagaa agatgttcag gaaaaaggcc ttatgggcag
      961 tggtcagtgc attcttgttc ctgctttttg ccgttacacc ctcgggcgca gcgaaccggg
     1021 actttagcaa gatcaacacg gtgaaaatgg ggattttagc ccctgttcag atgccggtgg
     1081 ggcacgggat catgaatgcc gccaagttag cagcggatga aatcaacgcg gccggtggga
     1141 ttcatgggaa aaaaatcgag ctggtcttcg gcgataccga atcgaagccc gaaaagggcg
     1201 tcacggccat gaagaaactg gtcttggaag acaaggtcga tgtcctggta ggagaataca
     1261 gcagcggggt ctctctggcc atccagcctt tcctttccgg ctataagatc gtttttatca
     1321 cgaccggctg tgcctctatc gccctcacgg acaatgtcaa aaaggactat gccaaaaata
     1381 aatatttttt ccggagtatg attaattcgg atatccgcca ggagaaatgg gccttcaaat
     1441 ttctaaagga atttgtcaac gggtccctgg ggtacaagag gtttgccatc ctggccgaaa
     1501 atgccaagtg gacggaggat tatgccccca acctgaaaaa agacctggag ggggcgggcc
     1561 tcgaagtccc cttcatggag aggtttgacg tggacatcaa ggacttctct cccatcttct
     1621 ctaagatcaa aagcctcaac gtccagtgga tcgcccagat tgtcagccat gcggccagta
     1681 tccccatggt gaaagcctgg gcggataaca agccggcccc catgggctgc gtgaacgtca
     1741 ccagccagga tgccaaattc tgggaaatga ccggcggggc ctgcctgggc gaagcgacgt
     1801 acaacttcat cgcccgggcg ccgttgaccg accgcacgat ccccttctgg gattcctatg
     1861 agaaaaaatt cgggaacaaa ccgatgtatc ccagcgggtt tacttacgac gccgtctata
     1921 tgctggctga tgtaatcaaa cagaagaaat cggtaaaatc cgatgacctc gtgaccgggt
     1981 tagaaaacat ttcttacaaa ggggtcatcc atccggaaac cgggtacgac aagcagagcc
     2041 atgacatcct ggaagggcgg tacgtgatgc ccgtggtgca gtggcaggcc ggtggaaaac
     2101 aggttgtgtt ctggcccgac cagtttaagg tggcccctta tgtgaagccc gaatggtgga
     2161 aacaataacc ctgaacgaat cggagagggt tcaataccgc attccccgtg ttgaaccctc
     2221 tttttgaatt tgccttctgc tcttcggtcc gcgggaaaat ttccagcgaa ttgcgaaatc
     2281 catcgaacag gttcccttgg agtgatttat gaccgcactg gtgagtattt ttgtcctggg
     2341 gacgatctgg ggggcggttt atagcctcat cgccgtggga tttaccctca tcttcggggt
     2401 ggccggaatc gtcaaccttt cccatggcgc cttttacatg ctgggggctt acctggccta
     2461 caccttcatg accctcctaa acattaacgt tcccctgtcc gccttgatgg ccgtagccgc
     2521 cgtggccttg atcggaatgg ccatcgacta tctcggcatc cggcccctgc gggaacgtca
     2581 cgcctatgtg ttgatcctga ccttggccct ggccctcttt tttcaggaac tcatgtacgg
     2641 gatctatggc ccctacggca agcccgtcaa gagtttcgtg gtcggggaga ttgtgctggg
     2701 aggagtgtat atttcctacc agaagctgat cactctattc gtcgccgccg ccttcgtaat
     2761 cctcctgtgg ttgttcatta agaagaccaa aaccgggaaa tccatctctg ccgtggcgca
     2821 gaacaaggat gcggcgatcc tgatggggat ccagtatgaa aaggcctacc ggatgaccat
     2881 ggggatttcc gcgggcctgg cggccatcgc cggggttttc atctccccga tcctggaggc
     2941 ggtccccact atgtggtcgt tccctctgtt taaggccttc gccatcgtga tcatcggggg
     3001 cctaggaagc ctggaggggg cgatcatcgc cagcctgctt ttgggctact ccgagacgct
     3061 ggtcagcttt ctcttctccg ctaactatgc ggacatggtg tatctgatcg ccatcattct
     3121 ggtcctggtc ctgcggccca ccggcatttt gagcaagggc cgccgggctt aagcggaaga
     3181 ctctgccgga aaggttcccc ggcatcgagg cgaaacccta ctatcggagg gagaaaatac
     3241 ggtatggaag gttatgaaga attattcggt ccccgaagat tctaccggag gctgctgggg
     3301 ttaggcattt tcagcgccat cgttctcctg gcccctctgg tggtggaaga tccctattac
     3361 ctggagctcc tgtttctctg ccattactat gtcatcctgg cctgcagctg ggacctgctc
     3421 tccggattca ccggaaacat caatttcggc cacgcctttt ttatcgctgg cgcgggatac
     3481 acgggggcgc tgttaaacct caacctgggc tggcagtact ggttgaccct gcccgcggga
     3541 ggaatcatcg ccgccctttt cgggctgcta gtgggcgtgg tcaccctgag gctgaaagga
     3601 ccctatttcg ccgcagttac tttttgcttc ggcgccctgc tttacaagtg gttcatggtc
     3661 agctccaagt ctctgggcgg cgaagagggc ctttcgggag tcgcttccct ctccgattcc
     3721 cccctgggga actactattt ttcgggcatt tttatgatcg ccgtcgtcct gggcctgtat
     3781 tttctttcca agtcctcctt cggcctgatc cttcgctcca tcggggaaaa cgaaacggcc
     3841 agcgaagggt ccgggattaa caccatctat tttaaggtgg tggccttcat gatcagcgcc
     3901 gcgatcgccg gtgtcgccgg ggccatgtac gcccacgccc agatgcatgt gggtccggag
     3961 atggcccatg actccctctc cgccctcgtg ctgatgatgg cggtggtggg ggggatgggg
     4021 tcgatcatcg gcccgatcat gggtgcctat ctcctgacga tggtgaatga atcgctccgc
     4081 ttcatggggg aattacgact gcttatctac agcggcgcgg tggtagcggt gatccttttc
     4141 gcccccaaag ggctcctgga aattctctcg caaggcttcc gacgcgtaac cggcgaccgg
     4201 cagaaggcgg tctgaggccc aagaggagaa atgggtatgg atgcagtgtt agagattcag
     4261 ggaatgtcca aggccttcgg aggccttcag gcggtcaaga attttgacct ccaggtacgc
     4321 aaaggcgatc tcattgggat catcggaccc aacggcgcgg gaaaaaccac tgttttcaac
     4381 atgattaccg gatttatccg gcccgattct ggggaggtta ggttccgggg ggaaaacatc
     4441 acccgcctga agccctttga gatcgtcaac cggggcctgt gccggacgtt ccaactggtc
     4501 gacctcttca aggagatgac cgccctggag aacgtcatga tcccctgctt ctcccaccgg
     4561 gtccgccagc gccgggccca agaggaaaag ccgtggaaaa ccgccatgaa attcctggaa
     4621 gaaatgggcc tggccaagcg gttcaacgag tacgccaaga acctgccctt cggagaattg
     4681 cgcctcctgg acatcgcccg ggccatggcg acccagcccg acattctcct cctggatgag
     4741 cccttctccg gcctagacgt ggaggatgcg agcgtcctct ccgcggcgat taagcagatg
     4801 catcagaaag gccagaccat gattattatc gaacatcggc tccgggacct gttgaagtta
     4861 gtggatcggg tggtggccat cgtctttggc gagaagatcg ccgaaggaaa ccctcaggag
     4921 atcatgaaca acccgaaagt ggtcaatgcc tacctgggag aaaggagaga ctgaattgca
     4981 ctttctggaa atgaaagcca ttaaggtctt ttatggaaag gccattgccc tgaatgggtt
     5041 gaccctgagc atcgatgaga aggaaatggt gggagtcgtg gggccgaacg gagcggggaa
     5101 gagtactctt ctacgggcta tttcggccct gatcccatcg gagggagata tcctgttcca
     5161 gggggaccgg atcgatcatc acccccccca cgagatcgtc cgcaagggga tcatccactg
     5221 ccccgagcgc agacagctct tcgtggattt taccgtcgcc gaaaacctgg aaatgggagc
     5281 tttcctgagc aaagacaagg agaagattcg cgaggacctg agccatgtct tccacctctt
     5341 tcccgtcctg gaagagcgga ggaaccaact ttccggcacc ctcagtgggg gagagcagca
     5401 aatgctggcc atcggtcgct ccctaatgag cagcccccgt cttctcatgc tggacgagcc
     5461 ttccatgggc ctctctcccc tggtgaagaa ccttcttgtc gaggggatca agaccatctg
     5521 gaagagcggg ctgacggttt tgatcgtcga gcaggacgcc agcctagccc tcaatctggt
     5581 ggagcgggtt tacatcctgg agcacggcaa agtgggtctg gagggaaaaa gccgggacct
     5641 gatgaacaac gaagaagtaa aacgggttta cttccagctt gggtgaagct tgtccctgct
     5701 tgctcccagt cagaatggcc gattcttgca ggggggctaa gttctgccgc ttcaatagat
     5761 gtttggtggt atacttgatc ggcgggcccc ttcttcatcc aggcctaggt ccttaagggc
     5821 ctgaaaccca aaggaaggaa tttaaccaac gatccaaagg ttcagccatg accagcttct
     5881 acgaaaataa accctggttg aaacagtatt ccccctgggt ccctcccgag ttggagattc
     5941 ccgaagaaaa tttggttcaa attctgaagc gttccgttac gagcgcgggt ggcacggcca
     6001 tctactattt ccatcgctcc atctcctata ctgatgtaga tcggtggtcg gattccctgg
     6061 cccgggccct ccaggacctg ggcctgaaac cgggaaaccg ggtcatcttc ctgatgcaga
     6121 acattcccca gttcatcatc gcccagtatg cggtctggaa ggctggggga atcgtcgtcc
     6181 ccttgaaccc catgtacaag gagaaggagc tcgactactt cttccaggat tccggagcga
     6241 gggccctgat cgccatctcc tccgcctatg aatcgtcggc ccggaacatt gtccgggaaa
     6301 gaaaaatgga aatcgtgatc accacttcgg gcctggaatt tctctcccca gacgagcccg
     6361 ttccccggat gcttcaggag gagaaataca cccgctttcc ggaagctatg gatttcatgg
     6421 acctcctgaa gaaatacgga gaagaggtcc cccacccgac ggaattgccc aaccatcaat
     6481 cggcttactt tacttatacc tctgggacca ccggccctcc caaaggtgcc atgaatactc
     6541 accggaacat cgctttcaac gccaagttct acaccacctt cctccggctg gcctcccctg
     6601 acgtgatctt cggggtggcc cccttattcc acgtcaccgg ccaggtcgcc cacatggcag
     6661 ccgcggctta tgcgggcatt ccgctcatcc tgtccttccg ctttgacccg gaggaagccc
     6721 tcaagctcat cgaacggtgg aaggccacca tgaccctggg ggcaaccacg gtctacatct
     6781 ccctcatgaa tcatccgaat ttcaggaagg cagcattctc ttccatgacc aaggtgtata
     6841 cgggcggagc cccggtaccc ccggcggtgg tggaaaactt cgagaaatcc tgcggcgcct
     6901 acatccataa tgcttacggt ctgaccgaaa ccaactctcc ttcccatttc actccctggg
     6961 gcatgcgcgc ccctgtcgat cctgaaaccg gcgccctttc ggtgggcatt cccgtttgca
     7021 actccctttc caaaatcatg gacctggaga cgggtaaaga agagctgcct cccggagaaa
     7081 ttggcgagat cgtggttcga ggtcccatgg tggtgcccgg atactggaac aagccggaag
     7141 agagcgccca tgcgatccgg gatggatggc tctacaccgg ggatgtggga aagatggatg
     7201 aacggggatg gttctacgtg gtggaccgga aaaaggacct catcaacgtc tccggcttta
     7261 aagtatggcc ccgggatgtg gaggatgttc tctaccagca cccggcggta cgagaggtag
     7321 cggtcatcgg cgtccccgac ccataccgag gggagacggt aaaggccttc gtggccctga
     7381 aagaggaatt caaagaccgg gtgaccccgg tggagttgat cgatttctgc aaggcccgga
     7441 tggctgccta taagtacccc cgagtggtgg agatcacccc cgaaatcccc aagacaacca
     7501 cgggtaaatt cctgaggcgg atcttgcggg aaaaggaaag ggataagacc tgagggggga
     7561 aagagttcaa aaatgcgaag gggtgagaaa atatggccaa acagtatagt cgactggaag
     7621 tccgaaaaag gctggaggcg gagataaaga aaaaggcgcc catcgtggcc gccggggcgg
     7681 gaatcggtct ttcggcgaaa tttgccgagg ccggagggtc cgatttgatc atcatttaca
     7741 actccggcct ctaccggatg gacggtcttc cttccatcgc cggttacatg ccattcgggg
     7801 acgccaacgc tatggtgaag gagatggggc tgcgccatgt cttacccatc gtccaaaaag
     7861 ccccggtgat tgccggcatc tgcgcctatg acttgacccg ggacatggag gtttttctta
     7921 aagagttgct ggccgtaggg ttctcggggg tgattaattt ccccaccgtg ggaagaatcg
     7981 atggtgactt ccgccagacc atggaagata tggggatgaa ttaccggggg gaggccgagg
     8041 tcatggggct ggccgggtct ttaggccttt tcaccatggc ttatgtcttc aacgccgagg
     8101 agtccaggat gatggcccga aaggggattg actcggtggt cgcccacatg aagaccacca
     8161 ctggcgggac cgtggggtcc aagcggccca tgggcctgga ggaagcagcg aagcggtgtt
     8221 cggctatctt caaggccgcc ctgtccgtca agcggaacgt gatctgtctg gcccatggcg
     8281 ggccgattga gggaccggcg gccacggaat atatctaccg gcatacccag gcagtggggt
     8341 ttgtcggcgc ttcatccatc gaacgaatcc cggtggagac tgcaatccag aacacgacgg
     8401 agatgttcaa gacccaaagg ctgaaaagat aaaaaatgaa aacaaatgtt tctcgcagag
     8461 gccgcagggt tcgcagagaa gaaagagaca aaaaggaatt gcccaatact ttttgttaat
     8521 gcaatttggc caattttggc tttctctttg tactctgcgg gctcgagcgg ggcgggcgag
     8581 agtccgtagg ggcgattcat gaatcgcccc tactagagcc tgagctattc ttttttatcc
     8641 tttatggaca agtccatcct tatcatcgga acttttgaca ctaaggccga agaactgaac
     8701 ttcgtcaagg atcaaattct ggcgataggt gagaaggccc tgactcttga cgtaggagcc
     8761 cttcagcctt ccccggatta cgtggatatt tccaatgaaa aggtggccgc tgccggtggc
     8821 cggccgattt atgaattggt gagccggaga gaccgtgggc aggcggtggc ggtcatggcc
     8881 cagggcgctg cgaagatcac ccgtgagttg ttcgatagag gagaaatctc cggcgtcatt
     8941 tccctggggg gcggttcggg aacggtcatc gggaccaggg cgatgaggac tctcccgttg
     9001 ggaattccca aggtcatggt ctctaccatg gcctccggaa atgtccgcct ttatgtgggg
     9061 atttcggata tctgcatgat ccactcggtg gtggacatct ctgggcttaa tcgcgttacc
     9121 aagaagattc tgggcaatgc ggccgctgcg gtgtgcggca t
//
LOCUS       DASPRR010000220        25365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2307915, whole genome shotgun sequence.
ACCESSION   DASPRR010000220 DASPRR010000000
VERSION     DASPRR010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 25365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25365)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25365
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307915"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VEL68_08505"
     CDS             complement(<1..420)
                     /locus_tag="VEL68_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36044.1"
                     /translation="MRFIRSNSWMLFLASFLIIFPWLFPLLSFVNIVSEMLIMALFAV
                     SLNLLIGYTGIISFGHAAFFAVGSYTVGIMLQKLSGSLPLAIPSVLLCAMIFSGIAAL
                     VIGYFCTRLTAIYFSFLTLAFSQIVYAVIIKWASFTGG"
     gene            complement(432..1334)
                     /locus_tag="VEL68_08510"
     CDS             complement(432..1334)
                     /locus_tag="VEL68_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36045.1"
                     /translation="MDLASLFNCLASPGCILGQVIGALIIAMVLFLVASGLSLILGVL
                     GVMNFAHGCLYMLGAYFTYTVVEHFGNFGLALLLAPIGVGITGVILERFFIKRVYGLP
                     DLYQLLLTYAFILVLDDMAKLIWGIFDLSVPVPGWFRRPPINIMGTLVPFYYLFIMLA
                     GAALVLGIWFFLAKTKFGKIINAAASDPEMLMCVGMNVPLVYTSVFALGSLLAGLGGV
                     IASPLRSIVPEMGNSIIIESFIIVVIGGMGNIIGTFLVALLFGLMRSFGILGFPFFEL
                     AFIYILMAVVLIIRPSGLFGRATV"
     gene            complement(1431..2744)
                     /locus_tag="VEL68_08515"
     CDS             complement(1431..2744)
                     /locus_tag="VEL68_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36046.1"
                     /translation="MEKSPKRKTGRKGLTRREFLKTSAVSLVGLGVGSHLSPRVAHAQ
                     KAPIRIEGISSFTGRSAVTGVTTRDAVLMWAEGLNAQGGLLGRKIEVRFRDSQVKIEE
                     STRLARAAAASKEADLLLDCCSSLESFGVKEVSRDLKMLTITGTSKTTELTADPKTFS
                     PFHFRSSPSNIHDMAAGAKYAADICKKNGWKRWAMIAPDYAYGRENVMFFVRFLKIFY
                     PEAEIVAEVWPKLYEPDFTPYINKIMGAKVDACFTSQWGGDIVALLEQGGLYGFSDKV
                     KIFSIDLGDFTAINPVIKAFGKFPPGIFMGTRSNPIVPDTKLNSDWFYAFQKRFNYEP
                     SGWSQQTYTACLFYQKAVEKAGTTDQNAVRAALENLEIVCPWGTPPGQKCVMRGRDHQ
                     IILYTEAWGKTISKMPYVEEVLTMSWDDIIKVESDWLKEKGWLKT"
     gene            complement(2842..3534)
                     /locus_tag="VEL68_08520"
     CDS             complement(2842..3534)
                     /locus_tag="VEL68_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36047.1"
                     /translation="MLQVKDIHTYYGTSYIVQGLSLEVKAGEIVCLIGRNGAGKTTTV
                     RSIMGLTPPRRGSIKFKGEEIMGKSPFTIIRRGVGFVPEDRRIFPRLTAKDNLEIAQR
                     NFRPDGWTLEKIYDFFPKLKELENHLGTEMSGGEQQMLAIARSLMGNPELVLVDEPTQ
                     GLAPLIIQSMVDLIKRLKEKMSILLVEQNVLFAFQISDRGYVIDKGKILYQGWISDLK
                     GNREVHDRYLAV"
     gene            complement(3534..4286)
                     /locus_tag="VEL68_08525"
     CDS             complement(3534..4286)
                     /locus_tag="VEL68_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36048.1"
                     /translation="MSILEIKDLSKNFGHVAALSNVTLSIREGEITSLIGPNGAGKTT
                     FYNLVTGKFPPTTGSINFRGEEIASLPPYKVWKKGISRSFQITNIFKTLTVLENIRSA
                     LIVHEGQSLNFFKPIDYIEDLYKKSLQILKLIGLEDQKNVPCYAISHGDMRIVEVGIV
                     LASEPKLVFLDEPTAGMNPEETKKMVTLIKNLFETTGTTFFLTEHDMNVVFSISHRII
                     VLNYGKILADGTPEEIRQNEAVKQAYLGGYQE"
     gene            4892..5986
                     /locus_tag="VEL68_08530"
     CDS             4892..5986
                     /locus_tag="VEL68_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXZ36049.1"
                     /translation="MKGKAAILMGPREIAIKEMDLPSVEPGAVLVEVVKANVCGSDVH
                     IWHGLHPVIKKGVVLGHEFIGRIFKLGEGVTTDYANTPVKVGDRIVAPYFLTCLKCPP
                     CLRGQFSLCLNAYKFTASPPETPPHFHGSYATHYYVHSNQYFYKVPDRLADAVAAGAN
                     CGLSQVYYGLDKAGLSMGETVVIQGAGGLGLYASAIAKEKGAKVVVIEGAAERMKFAG
                     AFGADHLIGLKEFPTVEERLEQVKKITGSDGADVVLEVTGVPSAFAEALQLVRIGGRV
                     IEIGNILIGPQFEVSFSPGLITRKAILVQGVVRYDPWYLHRSLQFLEKNQAKYPFEKF
                     SDREYSLGEAREALERSEKRSIARAIIAPK"
     gene            6136..6924
                     /locus_tag="VEL68_08535"
     CDS             6136..6924
                     /locus_tag="VEL68_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36050.1"
                     /translation="MDKTPSHLQIQGLSLHFGGVQALHQVDLEIPRGILFSLIGPNGA
                     GKTSLINCVSKFYEPNAGEILFNGREITRFRPHEVARSGISRTFQNIELFKGMTVLEN
                     IKLGRHIHLRSGALAGMVYLGKARREEEMLEAFLDREILKFLGLTPIKNRVVATLPYG
                     LQKRVDLARALSAKPEFLILDEPVAGMNREETQDMITHILDIKKAWGLTVLLIEHDME
                     VVMNISDWIVVMNFGQKIAEGTPALVQKNPEVIRIYLGHSYAET"
     gene            6938..7822
                     /locus_tag="VEL68_08540"
     CDS             6938..7822
                     /locus_tag="VEL68_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36051.1"
                     /translation="MELLIQVLIAGITIGSIYSLIALGVVLIFKSSGVINFAQGSMVM
                     VGAFITYALVVQFKLPILLSLALTFLIAGLMGMIIERFVLRYLAGASLISVIMVTMGI
                     SYIMDGGALATWGSSNFTYPKLFPPFSMQIGGVKVSSVYLWSFLASMVLLVVFLFFFK
                     YSKMGLSMRAAADNQKLAVVLGISARRVLSLTWIITSAVATAGGILLASISALHVNLS
                     YIGLVVFPVVILGGLDSVAGAVVGGLIVGSLEAIAGVYIAPYLGGAFQQVASFVFLLI
                     ILLIKPYGLFGTKEIERL"
     gene            7825..8877
                     /locus_tag="VEL68_08545"
     CDS             7825..8877
                     /locus_tag="VEL68_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36052.1"
                     /translation="MGLSNRKGRPYFLLFLAVFILFPFSLFQYTKYILFLVNMTAIGV
                     IGALGLNILTGYTGLISLGQAGFMGVGAYTVAILSTKVGLPMWINLPAAGIITTLVGI
                     IVGTPSLRLKGFYLAMSTLAAEFLLEYVFKNWESMTQGVQGLFITPPQVFGVHINNDR
                     VFYFLSVSLAILAVIGTRNLFRTNVGRAFIAIRDRDIAAELIGINVFKYKLVSFAISS
                     FYAGVAGGLWCYYMTIINPEAFQLSISIDYVAMIIVGGMGTLFGSVYGAIFVVLLPEL
                     IQRLMEVLQPVLHMEYTFAAFKQIVFGVIIVIFLVFEPKGLAEVLSRLGSKVSNRKRK
                     EAVQNESLPGTGNSKI"
     gene            8893..10062
                     /locus_tag="VEL68_08550"
     CDS             8893..10062
                     /locus_tag="VEL68_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36053.1"
                     /translation="MKRLVGLAVVCLIGFMGLGAYAAEIPVGVYGTLTGTDTDVNGMS
                     YGPKDYLTYINEKAGGVMGHKYVPVLLDGKNQIPEEVKVFKRLVDVDKVVVVNGWSTG
                     GTKALRDQINTLVKVPFQAQSLSKDVLDPEKYPFIYLLGPTYEDQVIIAMNWAKQKGA
                     KTFAFIHADIEYGRAPCRNAIEQKVPEKLGLKVLGDIEYPGASPDLTPQLLRIKEMNP
                     DYVYIQDSVNNCVKVLRDAAKVGVPASKFIGNNYDFSHIIPETLGKDAEGFKAIQINA
                     DWGENIPMMKEIEEYAKTHTIAKKDTYYTKGWVQGKVIHEVATRVLKKSSGKLPEISQ
                     FRLMFRDEWEQLSNFDTGGGTPPLTYKDHQGHTQARILEIRGAKYGSISDWIKTH"
     gene            10114..10848
                     /locus_tag="VEL68_08555"
     CDS             10114..10848
                     /locus_tag="VEL68_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36054.1"
                     /translation="MLLEFKDIEVLYSNVILALKGVSLEVPEGHVVTILGANGAGKTT
                     ILKAISGVLMLENGRVHRGEILFQGRRIDGMDPAEIARSKISLVMEGREIFRSLTVEE
                     NLRVGTLMRVEGKKEIRRDLELILSYFPRLQERKGQVAGTLSGGEQQMLVIAMALMTR
                     PKLLLLDEPSLGLAPLMAESVMGEVKKINDNLRTTILLVEQNASLSLPISQFVYIIAN
                     GRVALKSTAEEIKGKEISQYYLGSGR"
     gene            10954..12843
                     /locus_tag="VEL68_08560"
     CDS             10954..12843
                     /locus_tag="VEL68_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ36055.1"
                     /translation="MPNGYNGKILRVNLTSGAISEDTPPENVYRMYMGGSALSSYYLL
                     KEQKPGVDPLGPENKLIFMSSVISGAPLPGLTRYTVAARSPLTGAFGEAEAGGFFGPE
                     LKMAGFDGVIVEGRAAKPVYLWIKDGKAEIRDASKIWGKDTGEAERLIREELGDNRVR
                     VAQCGPAGEKLVRYACVVNELKHANGRTGMGAVMGSKNLRAIAVRGTQKPSPADPEKV
                     REIGKKVLELLPQSVLAQNLKKFGTPMFVMPLNNSGILPTRNFQKGQFEGAEAICGEK
                     MTDTILTKSEGCYACAVQCKRAVKVEGKYKASPEYGGPEYETVASLGSYCGIDNLAAI
                     AQGNEMCNRLGLDTISVGVSIAFAMELVEKGILTPRETDEIDLRFGNTDAMLAMIGKI
                     ARREGFGDLLAEGVQRAAQKIGKGTEKYAMHIKGQELPMHDPRGKQGLSLSYATSPTG
                     ADHIEAPHDTSFLTDGPALKASRPAGVMEPVPAMEMGPRKVRQFVHTQAIWNSFNSMG
                     VCNFAAAPYTAFPLVTLAEAVEAITGWNTSLYELMELGERTITMARMFNMREGLSSKD
                     DYLPERLYETLEAGTPREKKITREDFANTLRLYYQAMGWDPKTGVPTEGRLSFLGLDW
                     LIPKK"
     gene            13313..14122
                     /locus_tag="VEL68_08565"
     CDS             13313..14122
                     /locus_tag="VEL68_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ36056.1"
                     /translation="MFDLSKFSLQGKVAIVTGGGRGIGKAVAQGFAKAGAKVAITSRK
                     INDLEATAAEIRGFGGEALPVQAHLAKAEEISRMVGTVLGKFGRVDILVNNAGTSPAM
                     GSVLDSDERLWDTVINLNLKGLYYTSQAVARVMKKQGGGRIINVASIDGFKPEPGVSI
                     YSISKAGVRMVTQAFAAELAPYNIRVNAIAPGPISTKMLDSHWFNLPPEEAQKAKEAL
                     GKLTPLGRVGEPEEIAGAAIYLASDASSYTTGAEIVIDGGVLLGFAVPGVQ"
     gene            complement(14219..15058)
                     /locus_tag="VEL68_08570"
     CDS             complement(14219..15058)
                     /locus_tag="VEL68_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HXZ36057.1"
                     /translation="MTREKDQAQPRIIVKKKSSHGGHHGGAWKVAYADFVTAMMALFI
                     VLWIVGQSKTIKEMISQYFKDPGAFAEKVKNGGAGILPDYAPPGTQADPPPAVRLSKD
                     PDVDMKQLEEEKKKLEEMIRKTPVFDKFKGQVEITVSDEGLRIELVDNSSGLFFDVGS
                     AHLRKETLQLLGMIAGEIGKLPNRVIVEGYTDARPYVGPNYSNWELSVDRANTARKIL
                     VENGLRKDQVQEVRGFADQRLRRPENPMDFSNRRVSILVPTLPAPAPPPLNPSGVQVK
                     AGG"
     gene            complement(15055..15912)
                     /gene="motA"
                     /locus_tag="VEL68_08575"
     CDS             complement(15055..15912)
                     /gene="motA"
                     /locus_tag="VEL68_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HXZ36058.1"
                     /translation="MLAIVGILVVLLAVLGGYLIEGGSFLVLMQFAEFIIIGGAAAGA
                     LLISTPPKLLKKILNRVLLIFKKSKVEKETYLGILKMMYEFFQLSQKEGVLALESHIE
                     HPEKSTIFSKYPQFVGQHHLLSFLTDTMRIILMGSVSPYEIEALMDTDIETQQQEGTK
                     PGMILQKIGDSMPGLGIVAAVLGIVITMQAINGPPEQVGHKVAAALVGTFLGILLSYG
                     FIQPLATSLDLMHEEEARMLEVIKAGLVAFSKGFNPMIAVEFGRRSIFDDSRPTFREM
                     EQALRGGKA"
     gene            16477..16959
                     /locus_tag="VEL68_08580"
     CDS             16477..16959
                     /locus_tag="VEL68_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXZ36059.1"
                     /translation="MFKIGDKAVYPAHGVGVIEDIQCKVIGGCKRMFYVLRILEKDMT
                     IMIPKDNVQNVGLREIIQKKDVPKVFRILSEKNGKVDCQTWNRRYREYMEKIKSGSVF
                     EVAEVLRDLFHLKCDKDLSFGERQMLDLAQNLLVRELASAKNISEEKMRVRLLSIFNA
                     "
     gene            17010..18044
                     /locus_tag="VEL68_08585"
     CDS             17010..18044
                     /locus_tag="VEL68_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HXZ36060.1"
                     /translation="MGLLILRVLLFAATGASGYFLALQALSFPEAGLWGLGIGWALGL
                     LVILAERGIRRVPLKITIGGAIGLVLGLMLANVLMNSFFSGILEGLGIYFPGYFLINS
                     ALGYLGFLLGVKKGKEFELARLPWFSQRPGEGCDGHKVLDTSVIIDGRIADICDTGFM
                     EGTFIIPQFILQELQHIADSSDPLKRGRGRRGLDVLHRIQKQSNLEVRIVDQDYPKIQ
                     EVDAKLIALAKEISAKIVTNDFNLNKVAELQGIPVLNINQLFNALKPVVLPGEVMNVK
                     ILKEGKEPGQGVAYLDDGTMVVVDNARRHMGKSVDVTVTSVLQTTAGRMIFTILKEDA
                     PENFYQSINH"
     gene            18213..18950
                     /gene="ispD"
                     /locus_tag="VEL68_08590"
     CDS             18213..18950
                     /gene="ispD"
                     /locus_tag="VEL68_08590"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXZ36061.1"
                     /translation="MRVVALIPAAGQGKRMGTERPKAFISLGVLPILAHTLRKFEACP
                     QVDEVIPLVPPGEGVAWTREIVSQGGLQKVSQIMPGGAERQESVFIGLRAIREKAEAV
                     IIHDGVRPFVTPDLIQRALSEARRFQAVAVALPASETIKEVARENTVLRTVDRRQLWM
                     MQTPQSFDYELICRAHEEARRDGFVGTDDASLVERLGIPVKIIEGSRFNFKITTPEDL
                     VLAEALLKYSSEFGVKGFSNGGLSDFH"
     gene            19033..19524
                     /gene="ispF"
                     /locus_tag="VEL68_08595"
     CDS             19033..19524
                     /gene="ispF"
                     /locus_tag="VEL68_08595"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXZ36062.1"
                     /translation="MRIGFGYDVHPLVEGRKLILGGVEIPFEKGLEGHSDADVLLHAI
                     CDGILGALAEGDIGKHFPNTDPRFKGISSLRLLEGVASKLAQKFFLVENLDSTVVAEN
                     PKIGPYIPRMAEQIARVLRIAPQQVNIKATTSENLGFIGEGKGIAAYAVVLLKEESGK
                     VSS"
     gene            19731..21188
                     /gene="gltX"
                     /locus_tag="VEL68_08600"
     CDS             19731..21188
                     /gene="gltX"
                     /locus_tag="VEL68_08600"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXZ36063.1"
                     /translation="MNVRVRFAPSPTGLLHLGNARTALFNFLFARQTGGTFILRLEDT
                     DRERSTPEAEKAVLEDLRWLGLEWDEGPERPGDFGPYRQSERLETYRRYAMDLLEKGH
                     AYRCYCTDEELEEKRQRSLAKGIPPMYDGRCRYLKPEEEQSFIRSGRPASVRFRVNAR
                     SAEFQDLVKGAMSFDGRKMGDFVILRSDQVASYNFAVVIDDARMEVTHVIRGEDHLAN
                     TARQILLYQTLGFAPPQFAHLSMILGPDRTPLSKRHGATAVAHFREAGYLPEGFLNYL
                     ALLGWSSEDGQEIFSLGELIKKFSLQRLSRSPAVFDPEKLNWMNRAHLKGIHGERALN
                     LALPFLRRSGEKLEAVPKSWLVAALEAVWGEVDTLSQLAERLRFLFDEGWTLEPDAET
                     LVVKEETQRVIRGLREELRSVEEVGPENYRALLAGLAKRVGLTGRNLYMPLRAALTGR
                     TQGPELEKVFLLLGKEKILKRLDSILQSNGKERSL"
     gene            21190..22218
                     /gene="rfaE1"
                     /locus_tag="VEL68_08605"
     CDS             21190..22218
                     /gene="rfaE1"
                     /locus_tag="VEL68_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HXZ36064.1"
                     /translation="MKNQDLISFISKFKNARILVIGDLMVDEYIWGSVSRISPEAPVP
                     VVSVTSESLRLGGAGNVVNNIYTLGGKVWIGGIVGNDEMGRKVIQDLHRMGVDTRAVA
                     VESDWITTVKTRIIAHHQQVVRYDREVVRSIRPEALKKILSVVEDRVREFDAVLISDY
                     GKGVICPELVEQVRSLALGAGKILTVDPKVKNFPLFQKVTVITPNHHEAAQATGRWIQ
                     NEEDMVSVGRQLLGQLQAQSVLITRGEKGMTLFQENGEITNIPAMAKEVYDVTGAGDT
                     VISVLTLAMSVGASVKEAAVLSNHAAGIVVGEVGTATLKASELEDAVKHGVRSKSNPS
                     VPNAPGAP"
     gene            22215..22781
                     /locus_tag="VEL68_08610"
     CDS             22215..22781
                     /locus_tag="VEL68_08610"
                     /inference="COORDINATES: protein motif:HMM:NF025743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HXZ36065.1"
                     /translation="MSFPRPPKAVKLAMSFICSRGEDFASAIAEADPEYGPVDLVSEP
                     LSFHFTDYYEREMGKGLWRRMVGFEPLIAPEQLVSIKLWANRVESQSRNEQGGRRVNI
                     DPGYVAGSKFVLATGKDYSHRIYLGGGIYGEITLIVPKGGLSPLPWTYPDYASQPLLG
                     IIDLLRKRYLWQIRQQGERGERGNGEKS"
     gene            22835..23713
                     /locus_tag="VEL68_08615"
     CDS             22835..23713
                     /locus_tag="VEL68_08615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXZ36066.1"
                     /translation="MIKSMTGYGRGEWQGDGKKLGVEIRAVNHRYCDISIRLPRRLNP
                     LETQLRNLLRQRISRGRIEVSVQLDESSLPEQKLELDLPLARDIYLALKKLQENLGIP
                     GEVRLETMAGFRDIFGRKEAEPNLEKEWECLRTALEDALSGLEAMRGEEGRKLREDFL
                     GRLAAIEKMVQQIEEKAPLALRACRDRLAQRVQELSGGLEIDETRLAQEVGFLAERSD
                     ITEELVRVRSHLAQFRDLLGRPEPTGRKLEFLLQEINREANTIGSKANDASIAHVGVE
                     IKSELEKMREQVQNVE"
     gene            23895..24509
                     /gene="gmk"
                     /locus_tag="VEL68_08620"
     CDS             23895..24509
                     /gene="gmk"
                     /locus_tag="VEL68_08620"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXZ36067.1"
                     /translation="MKKNEGILFVVSGASGTGKSTLCRAMLRSFPNLRFSISHTTRPR
                     RPGDREGRDYFFVPPQQFQTMIDQGEFLEWAEVYGHRYGTSRRMLEMARAEGQDLILD
                     IDGQGARQLRDQNIPGAFVFILPPSLEELKRRLMNRKTEGKEALEERVKKARAEIADS
                     HWYDYVIINDYLKAAKARLQAIILAEHCRREKLTEAIDKLLVEK"
     gene            24775..25014
                     /gene="rpoZ"
                     /locus_tag="VEL68_08625"
     CDS             24775..25014
                     /gene="rpoZ"
                     /locus_tag="VEL68_08625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXZ36068.1"
                     /translation="MARITVEDCLKKVSNRFALVLLAVARTKQLYKGSKPRVQTDNKE
                     VVLALREVAAGKVMPARPLQEGLQVQGKSPKQLKE"
     gene            25032..>25365
                     /locus_tag="VEL68_08630"
     CDS             25032..>25365
                     /locus_tag="VEL68_08630"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HXZ36069.1"
                     /translation="MNPSSSAAGKVIFALDVPTLEEARKFVRLLKGRTGFFKVGLELH
                     TAYGKEAVKAVQEEGGRIFLDLKFHDIPNTVSRAAEEAVKLGVDMFNLHASGGTEMVR
                     ETVERCRKT"
BASE COUNT         5885 a         6667 c         7185 g         5628 t
ORIGIN      
        1 tcctccggta aagcttgccc atttgataat taccgcatag acaatttggc tgaaggccag
       61 ggtaagaaag gagaaatata tggccgtgag tcgagtacaa aaatagccga tcaccagggc
      121 cgcgattccc gaaaaaatca ttgcgcaaag cagcacggac ggtatggcca gcggcaaaga
      181 acccgaaagt ttttgaagca tgatcccaac tgtgtacgac ccgacggcga aaaaagccgc
      241 atggccgaag gagatgatcc ctgtgtatcc aatcagcaaa ttaaggctta ccgcaaaaag
      301 agccatgatg agcatttcgg aaacgatgtt cacaaaggat aataggggga ataaccaagg
      361 aaagatgatt aagaaactgg ccaaaaacag catccagcta ttggatcgaa tgaatcgcat
      421 atccttcctc ttcagaccgt ggccctgccg aataatccgg acgggcggat gatcaggaca
      481 acggccatca aaatgtagat aaaagccagc tcaaagaagg ggaatcccag gattccaaag
      541 gatcgcatca acccgaatag aagggcaaca agaaaggtgc cgataatatt ccccattcct
      601 ccgatgacta cgataataaa cgattcgatg ataatcgaat ttcccatctc cggaacgatg
      661 gaacgtaagg gggaggcgat gaccccgccc agccccgcca agaggcttcc cagggcgaaa
      721 accgaagtgt acaccagagg aacattcatg cccacgcaca tcaacatctc cgggtcggaa
      781 gccgcagcat tgataatttt gccgaatttc gttttggcca aaaagaacca aatccccaag
      841 accagcgcag cgcccgccag cataataaat aaatagtaaa agggaacaag cgttcccata
      901 atgttgattg gaggccgacg aaaccagccg gggaccggaa cggataggtc aaagatcccc
      961 caaattaatt tggccatgtc gtccaagacc aaaataaagg cgtaggtgag caggagttga
     1021 taaaggtcgg gtagtccata gaccctttta atgaaaaaac gctccaggat gaccccggtg
     1081 attcccaccc caattggggc cagcaataaa gccaggccga aattcccgaa atgttccact
     1141 accgtatagg taaagtaagc tccaagcatg tataggcatc catgggcaaa attcatgacc
     1201 cccaggaccc ccaaaatgag gctgagcccg gaagcaacga gaaacaacac catggcgatg
     1261 attaacgcac cgatgacctg ccccaaaatg cacccgggac ttgccaaaca gttgaataaa
     1321 ctcgctaggt ccatcgatat tctcccgaaa gtctttcccg ccctcgaatg gcgccgcaaa
     1381 ccagactgaa gaggagccct ggaaggtacg gccagggctc cttccgccaa tcaagttttg
     1441 agccaacctt tttccttaag ccaatcactt tcgaccttta taatgtcatc ccaggacatg
     1501 gttaatacct cttccacata cggcattttc gagatggttt ttccccatgc ctccgtgtaa
     1561 aggattatct gatggtccct ccccctcatg acgcattttt ggcccggagg ggttccccag
     1621 gggcaaacaa tctccaggtt ctccagcgcc gcccggaccg cgttctggtc tgtagtgcca
     1681 gccttttcaa ccgctttttg gtagaagagg caggccgtat acgtctgctg ggaccatccg
     1741 gaaggttcat agttgaatcg tttttgaaag gcatagaacc aatcgctgtt taacttggta
     1801 tccggtacga tgggattcga cctcgtgccc atgaaaatcc ccggcggaaa ttttccgaag
     1861 gccttgatga ccgggttgat ggccgtaaaa tcccccaggt cgatgctaaa gattttcacc
     1921 ttgtctgaaa atccatacag gcctccctgc tccaataagg ccacaatatc tccgccccat
     1981 tgagaggtaa agcaggcatc gaccttggcc cccatgatct tattgatata aggggtaaag
     2041 tcgggctcgt aaagcttggg ccaaacctcg gcgacgattt ccgcctcggg ataaaagatt
     2101 ttcaggaacc ttacgaagaa catcacgttc tcccggccgt aggcataatc cggagcaatc
     2161 atggcccacc gcttccagcc attctttttg cagatatcgg cggcatattt ggccccggcg
     2221 gccatgtcgt ggatgttgga gggagaggac cgaaagtgaa aaggggagaa agtcttcggg
     2281 tctgcggtga gctcggtggt tttcgaggtt ccggtgatgg ttaacatctt gaggtcgcgg
     2341 gagacttcct ttaccccgaa agactccagg ctgctgcaac aatcgaggag aaggtccgct
     2401 tccttggagg ccgccgctgc tctggcaagc cgggtggatt cctcgatttt tacctggcta
     2461 tcgcggaatc ggacctcgat tttccggccc aaaagcccac cttgggcgtt gagtccttcc
     2521 gcccacataa ggaccgcatc tcgggtggtc acccccgtca cggccgacct tccggtaaag
     2581 ctgctgatgc cttcgatccg aatcggtgct ttttgagcat gagcgacccg agggctcagg
     2641 tgtgatccca cacccaaccc caccagggag acagccgagg tcttcaaaaa ttctcgccgg
     2701 gtcaacccct ttcttccggt cttccttttt ggagattttt ccatttttcc ctccttactt
     2761 tgtttttttg actttacggc cagccctgaa gatatttgaa aaggctggcc ttaaagcaat
     2821 cctctaactt ttggggaaca gtcaaacggc cagataccgg tcgtgaactt cccggtttcc
     2881 cttcaagtca ctgatccagc cctgatacag gatcttgcct ttatcgatca cgtaacctcg
     2941 gtccgagatt tggaaagcaa aaagaacgtt ttgctccacc agaaggatgc tcattttttc
     3001 cttgagacgt ttgatcaaat ctaccatgga ctggataatg agaggagcca ggccctgagt
     3061 cggttcatcc accaaaacta actccgggtt tcccatcagg ctgcgcgcaa tggcgagcat
     3121 ctgctgctcc cctccgctca tttccgtgcc caaatgattt tccaattcct tgagttttgg
     3181 gaaaaagtca tagatctttt ccagagtcca tccgtctgga cgaaaatttc tttgggcaat
     3241 ttctaaatta tccttggcgg taaggcgcgg gaagattctt ctatcttccg ggacaaaccc
     3301 aactcccctg cggatgatgg taaaggggga cttccccatg atttcttcgc ctttaaactt
     3361 gatggaaccc cggcgggggg gggtcaggcc catgatgctt cttaccgtcg tcgtttttcc
     3421 cgccccattc ctgccgatca ggcagacgat ctccccggct ttaacttcca gggagagacc
     3481 ctggacaata tagctggttc cataataggt gtggatatct ttcacctgca gcattattct
     3541 tgataccctc ccagataggc ttgtttaacg gcttcattct gccgtatttc ttcgggcgtc
     3601 ccgtcagcta ggatttttcc ataattgagt acgatgattc tgtgggaaat ggaaaagacc
     3661 acgttcatgt cgtgttcggt gagaaagaaa gtggttccgg tggtctcaaa gaggttttta
     3721 atgagggtta ccattttttt ggtttcttca ggattcattc ccgcggtggg ctcgtccagg
     3781 aagaccagtt tcggctcgct agccaaaaca attcccactt cgacaatcct catgtctcca
     3841 tgggaaatgg catagcaggg aacatttttc tggtcttcca gtccaatcag tttcagtatc
     3901 tgcaagctct ttttgtataa atcctcgata tagtctatgg gtttgaagaa gttcaggctt
     3961 tgtccctcat gaacgattaa agccgaacgg atgttttcca gaaccgtcag ggttttaaaa
     4021 atattggtaa tctggaaaga cctggagatt ccttttttcc agaccttata gggaggaaga
     4081 ctggcaatct cttcccctcg gaaattgatg ctgccggtag taggagggaa tttgcccgtt
     4141 actagattat aaaaagtggt cttccccgca ccgttgggtc cgatgagaga ggtaatctct
     4201 ccttcgcgaa tcgacagagt gacatttgaa agagccgcta catgaccgaa atttttagac
     4261 agatccttga tttccagaat actcatgatc ccccatccaa ttcaagccga gaataaagcc
     4321 atttggtatt ctcgccgcgc tgctggaatg aatgattctt ttagaactcg aaccttaaaa
     4381 cccctgcatt tggaggtcgc ctcgcgagat cagagaagtc cgaacatttt cctggaggag
     4441 taaagaagac tctgcgcgca agggacgctg tggaaatcgt gaatcggcgc aggtatgatc
     4501 cggaactcct cccgctgatt ctaggctcgt tgtcaagtct tccctggaaa aactggttca
     4561 ttctaatgaa tcgtccaaaa aggtcaagtg gaaaatctca gaggaggtct tttttttaat
     4621 ttttctcttg acatcatttg ggaatgaata taaagaactt ctagctttga gtagtctttt
     4681 ccttttggaa gaaaaaaaga atggtaacgt acccttcgag aagtctcaag tttaaggcat
     4741 tgccccaatc ccgcgcctag cttaggacct tgggatcggt ctttaggctg gcgtggtgtt
     4801 cttccaaaga acttttgggg cagaatttgg aaggcagggg aacgggtcca aaaaggaaac
     4861 ccgagtaaat attcggaagg gaggtatttc tatgaaaggg aaagcggcaa ttttgatggg
     4921 tccaagggaa atcgcgatta aggaaatgga cttaccttcg gtggaacccg gggccgtttt
     4981 ggtggaggtg gtaaaggcga acgtttgcgg ttccgacgtg catatctggc acggtcttca
     5041 cccggtcatc aagaaggggg ttgtgctggg tcatgagttt atcggtcgga tttttaaatt
     5101 gggggaaggg gttacaaccg attatgccaa cacaccggtc aaggtcgggg atcgaatcgt
     5161 ggcgccttac ttccttacct gcctcaaatg ccccccctgt ttgcgcggtc aattctccct
     5221 ttgcctgaat gcctataagt ttaccgcgtc tccaccggag acgccgccgc attttcatgg
     5281 ctcctatgcc acccactatt atgtccactc caaccaatac ttctacaagg tccccgatcg
     5341 cctggccgac gcggtggcgg cgggggctaa ctgcggcctc tcccaggtgt attacggatt
     5401 ggataaagcc ggtttatcca tgggggaaac ggttgtcatc cagggagccg ggggcttggg
     5461 tctctacgcg agcgccatcg ccaaagaaaa gggggcaaaa gttgtcgtga ttgaaggggc
     5521 ggcggagaga atgaaatttg ccggagcctt tggtgcggat cacttaatcg gcttgaagga
     5581 gtttcctacg gtcgaggaga ggctggagca ggtgaagaaa atcaccggaa gcgacggggc
     5641 cgatgtggtc cttgaggtca ccggggttcc ttcagccttc gcggaggctt tacagctcgt
     5701 gcgcatcggc ggaagggtga ttgaaatcgg caatattctt atcgggcccc aatttgaagt
     5761 atccttctcc ccggggctga tcacccggaa ggcgatccta gtgcaaggcg tggtgcgcta
     5821 tgacccgtgg tacctgcacc ggtcactcca gttccttgaa aaaaatcagg cgaaatatcc
     5881 ctttgaaaaa ttctccgacc gcgaatactc tctgggagag gccagagaag ctctcgagcg
     5941 ctctgaaaaa agatcgatcg cccgagcgat catcgctccc aaatagagga tgaaaaccgg
     6001 aatgaatgat atccggccca tggccgaagc ccccggtgca ggtgaccaga ctcctgagtt
     6061 ttaatcgccg gagggtgacc gtggccgcac tccaagaaat tttcgaccat gttttctgat
     6121 ggccttcgga ggcccgtgga caaaacgcca tctcatctgc aaattcaggg cctttccctg
     6181 catttcgggg gagtccaggc tcttcaccaa gtcgacctgg aaatcccccg gggaatcctc
     6241 ttttcattga tcggtcccaa cggggctggg aaaaccagct tgatcaactg cgtttccaaa
     6301 ttctatgaac ccaatgccgg agagatcctt ttcaacggcc gggaaattac ccgcttccgc
     6361 ccccatgaag tggcccgctc cggaatctcc cgcaccttcc agaatatcga gctctttaag
     6421 ggaatgactg tcctcgaaaa catcaagctg ggaagacaca tccacctgcg gtcgggagct
     6481 cttgcgggca tggtttacct gggcaaagcc cggcgggaag aggaaatgct cgaggctttc
     6541 ctcgaccggg agattctgaa atttcttgga ttgacgccta ttaaaaaccg ggtggtggcc
     6601 accctgcctt acgggctgca gaagcgggtg gacctggcca gggcgctctc tgccaagccg
     6661 gagtttttga tcctggatga gccggtcgcg ggaatgaacc gggaggagac ccaggatatg
     6721 atcacccata ttctggacat caaaaaggcc tgggggctga cggtgctcct cattgaacac
     6781 gatatggaag tggtcatgaa catctctgac tggatcgtgg tgatgaattt cggccaaaag
     6841 atcgccgagg ggacgccggc tctggtccag aagaacccgg aggtcatccg catctacctg
     6901 gggcattcct acgcggagac gtaggggaag cgaaatcatg gaacttttga tccaagtcct
     6961 gatcgccggg attaccattg gcagtattta ctcgctcatt gccctggggg tcgtgctgat
     7021 cttcaaatcg agtggggtga taaactttgc gcagggctcc atggtcatgg tgggcgcttt
     7081 catcacctat gccctggtgg ttcaattcaa gctccccatt ctgctcagcc tcgccctaac
     7141 cttcctgatc gccgggctga tggggatgat catcgaacgc ttcgtcctcc ggtacctcgc
     7201 cggagcctct ctcatctcgg tcatcatggt gaccatgggg atctcctaca tcatggacgg
     7261 gggcgcgctg gctacctggg ggtccagtaa tttcacctat cccaaacttt tccctccttt
     7321 ttccatgcag atcggggggg tcaaggtctc ctcggtctac ctctggagtt tcctggcctc
     7381 gatggtcctg ctggtggtat tcctcttctt cttcaagtat tcgaagatgg gtctgtccat
     7441 gcgggccgcg gcggacaacc agaaattggc ggtggtcctc gggatcagcg cccggagggt
     7501 cctttctctt acctggatca tcacttcggc agtggccacg gcagggggaa tcctgttggc
     7561 cagcatcagc gctctacacg tgaatctcag ctacatcggg ctggtcgtct tcccggtggt
     7621 tatcctgggg ggattggaca gcgttgccgg ggcagtggtc ggcggtctca tcgtggggag
     7681 tctggaagcc attgctgggg tttacattgc cccgtacctg ggaggggctt tccagcaggt
     7741 ggcgtccttc gttttcctgt taatcatcct gctgatcaag ccctacgggc ttttcgggac
     7801 gaaagaaatc gagaggcttt gaaaatggga ctttccaatc gcaaaggacg gccctatttc
     7861 ctgctttttc tggcggtctt catcctcttt cccttcagcc tttttcagta caccaaatat
     7921 attctattcc tggtgaatat gaccgccatc ggggtgatcg gagcactggg gttgaacatc
     7981 ctgaccggtt acaccggact catttccctc gggcaagccg gcttcatggg ggttggagct
     8041 tatacggttg ccattcttag cacgaaggtg gggcttccca tgtggatcaa cctgcctgcg
     8101 gccgggatca tcacaaccct tgtggggatc atcgtcggta ctccctccct gcggctgaag
     8161 ggtttttatc tggccatgtc caccctggcc gccgagttcc tgctagagta cgtcttcaag
     8221 aactgggaaa gcatgaccca gggggttcag ggcctcttca tcaccccgcc ccaagtcttc
     8281 ggcgtccaca tcaataacga ccgggttttc tactttctct ccgtgagcct ggctatcctg
     8341 gcggtcatcg gcacgcgcaa tctcttccgc accaacgtgg gccgcgcctt cattgccatc
     8401 cgcgatcggg acatcgctgc ggaattgatc ggcatcaacg tcttcaagta taagctcgtc
     8461 tccttcgcca tcagctcctt ctatgcgggg gtggccggcg gtctctggtg ttattacatg
     8521 accatcatca acccggaagc ctttcagctc tccatttcca tcgattacgt ggccatgatc
     8581 atcgtcgggg ggatgggcac cctcttcggt tctgtttacg gggccatttt cgtcgttctc
     8641 cttccggaac tgatccagcg tctcatggaa gttctgcagc cggtgttgca catggaatat
     8701 accttcgcgg ccttcaaaca gatcgtcttt ggggtgatca tcgtcatctt tctggtcttt
     8761 gagcccaagg gtctcgcgga ggtcctttcg aggttgggaa gcaaggtttc caatcggaaa
     8821 agaaaggagg cggttcagaa cgagagtctg ccgggcactg gaaacagcaa aatatagaaa
     8881 ggagggcttt ttatgaagag gttggtaggg ttggcggtgg tgtgcctgat cggtttcatg
     8941 ggcctcggcg cctatgccgc ggaaatccct gtaggggtct acgggaccct aacggggacg
     9001 gacacggacg tgaatggaat gtcctatggt ccgaaggatt atctgaccta tatcaacgag
     9061 aaagcgggcg gggttatggg tcacaaatac gttcctgtgc tgctggatgg gaagaaccag
     9121 attccggagg aggtcaaggt attcaagcgg ctggtggacg tggacaaggt ggtggtcgtc
     9181 aatgggtggt cgacgggcgg aaccaaggcc ctgcgggacc agatcaacac cctggtcaaa
     9241 gtccccttcc aggcccagtc gctttccaag gatgtcctgg acccggagaa gtaccccttt
     9301 atttatctgc tcgggccgac ctacgaggac caggtcatca tcgccatgaa ctgggccaag
     9361 cagaaggggg ccaagacctt cgccttcatc cacgcggaca tcgagtatgg gcgggccccc
     9421 tgccggaatg cgatcgaaca gaaggtaccg gagaaactgg gattaaaagt cctcggggat
     9481 atcgaatacc ccggagcttc cccggacctg actccccaac tcctgcggat caaagaaatg
     9541 aacccggact atgtctacat tcaggattcg gtgaacaact gcgtgaaggt gctcagggac
     9601 gccgccaagg tgggagtccc ggcctccaag ttcatcggga ataactacga cttcagccac
     9661 attattcccg aaaccctggg caaggatgcg gaggggttca aggccatcca gatcaatgcc
     9721 gactggggcg agaatatccc catgatgaag gagatcgagg aatacgccaa gacacacacg
     9781 atcgccaaga aggataccta ttatacgaag ggctgggtgc agggcaaggt cattcacgag
     9841 gtggctaccc gggtgctcaa gaagagcagt gggaagctcc cggaaatttc ccagttccgc
     9901 ctgatgttca gggatgaatg ggaacagctg agtaatttcg acaccggcgg gggaactccc
     9961 cctcttacct acaaggacca ccagggtcat acccaggccc ggattttgga aatccggggc
    10021 gccaaatacg gttcgatcag cgactggatc aaaacgcatt agaaggccag gggggaggga
    10081 atcggcctcc ccctttttct ccgggaagag cccatgcttc tggagttcaa ggatatcgag
    10141 gttctttaca gcaacgtcat tctggccctt aagggtgtga gcctggaggt ccccgagggc
    10201 catgtcgtca ccatcctcgg ggcgaacggg gccggaaaga caacgattct gaaggctatt
    10261 tccggggtcc ttatgctgga gaatgggcgg gtccacaggg gggaaatcct cttccaagga
    10321 cgccggatcg acgggatgga cccggcggag atcgcccggt cgaagatctc cctggtcatg
    10381 gagggccggg agatcttccg ctccctcacg gtggaagaga atttaagggt ggggaccctc
    10441 atgcgcgtcg agggcaagaa ggaaatccgc cgggacttgg agctcatcct gtcctatttt
    10501 ccgcgcctgc aggagagaaa agggcaggtg gccggcacgc tgagtggggg cgagcagcag
    10561 atgctggtaa tcgccatggc cctgatgacc cggccaaagc ttttgctctt ggacgagcct
    10621 tcgctgggcc tggccccgct gatggcagaa tcggtgatgg gcgaggtaaa aaaaatcaac
    10681 gacaatttaa ggacgaccat tctccttgtt gagcagaatg cctccctttc tctgcccatc
    10741 tcgcagttcg tctatatcat tgccaatgga cgggtggcct tgaagtcgac cgccgaggag
    10801 attaagggaa aggaaatcag ccagtactat ttgggaagcg gaagataata tctgtcgggg
    10861 ggccaccgat gtccgaaaga aatcttgacc ctggccggca aagataatag aattttatcg
    10921 gagtagattt cgagaattaa gggaggattc aatatgccga atggatacaa cggaaaaatc
    10981 ctacgggtca acctgacttc cggagcgatc agtgaggaca cccccccgga aaacgtctac
    11041 cgcatgtaca tgggggggtc cgctctttct tcctattact tgttgaagga gcagaaaccc
    11101 ggtgtagacc ccctcggacc cgaaaacaag ttgatcttta tgtccagcgt gatctccggg
    11161 gcgccgctgc cgggcctgac ccggtacacg gtggcggccc gatctcccct gaccggtgca
    11221 ttcggggaag cggaagcggg ggggttcttc gggcccgagc tgaagatggc gggatttgac
    11281 ggagtcattg tggagggccg tgcggcgaag ccggtgtatc tctggatcaa agacggcaag
    11341 gcggagattc gggatgcttc caagatttgg ggaaaagaca ccggggaggc ggaacggctg
    11401 attcgggagg aactgggaga caaccgggtc cgcgtggccc agtgcggccc ggccggggag
    11461 aagctggtgc gctatgcctg cgtggtcaac gagctgaagc atgccaacgg ccgcacgggg
    11521 atgggagcag tgatggggtc caagaacctg cgggccattg ccgtccgggg aacgcaaaag
    11581 ccttcgcccg ccgatccgga aaaagtccgg gagatcggga aaaaagtcct cgagctcctt
    11641 ccccaatctg tcctggccca gaatctgaaa aaattcggga cccccatgtt tgtcatgccc
    11701 ctgaacaatt ccgggatcct gcccacgcgc aatttccaga aaggacaatt cgaaggggcg
    11761 gaggccattt gcggggagaa gatgaccgac actatcctga ccaaatcgga gggttgctac
    11821 gcctgcgcgg ttcagtgcaa gcgggcggtc aaggtggaag gcaaatacaa agcctctccc
    11881 gaatacggag gcccggaata cgagacagta gcctccctcg ggtcctattg cggaatcgat
    11941 aacctcgcag ccatcgccca gggcaatgag atgtgcaacc ggttgggatt agacaccatt
    12001 tccgtgggag tttccatcgc ctttgccatg gaattggtgg aaaagggaat tcttaccccg
    12061 agggaaacgg atgaaatcga ccttcgattc ggcaatacgg atgcgatgct ggccatgatc
    12121 ggcaagatcg ccagaaggga agggtttgga gaccttttgg cagaaggggt tcagagagcg
    12181 gcccaaaaga tcggcaaagg gaccgaaaaa tatgccatgc atatcaaggg gcaggaactg
    12241 cccatgcatg atccgcgggg aaagcaagga ttgtccctga gttatgccac ttcgcccacc
    12301 ggggcggatc atatcgaagc tccccatgat acttcctttt taaccgatgg cccggcgttg
    12361 aaagcatcca ggccagctgg cgtcatggag cccgttccgg cgatggagat gggacccaga
    12421 aaggttcgcc agtttgttca cacccaggcg atctggaact ccttcaactc catgggagtc
    12481 tgcaactttg ccgcggctcc ttataccgct tttcccctag ttacgctcgc cgaggcggtg
    12541 gaggccatta ccggctggaa taccagcctt tatgagttga tggaactggg ggagcggaca
    12601 atcacgatgg cgaggatgtt caacatgcgg gaaggcctat ccagcaagga tgattatctc
    12661 cccgagcggt tgtatgagac cttggaagcc gggacccccc gggagaaaaa gattacccgg
    12721 gaagatttcg ccaatactct gagactttac taccaggcca tgggatggga cccgaaaacc
    12781 ggggtgccga ccgaaggacg gctcagcttc ttgggcttgg attggttgat tcccaaaaaa
    12841 tagataaaga ctctacaggt ccaaggccat agaagatcag gctttcctga attttctatg
    12901 gcctttttct tccctgaaac ttgggatctt tcggcagggt ctattgaatc acgacctctg
    12961 acagaggctt ggaaaacagg gaaccgttca aagcaaataa tgaatggcgt cacccaaagg
    13021 gggtcttccc cccgtttccc ggcccggggg caagggcatt tcatgcagaa atcatggggc
    13081 gcttttgatt ccatggaacg ggtgggcaca gcgtttgaac tccgtgcttg ggtccgttcc
    13141 ttgtaagggc gatccggcgg gtcgccccag ggcccgccgc ccattgcgga aatccggcga
    13201 agcgtctggg aaaagggctg acccggccga tgctttctgc atgcaatgcc ctcgccgccg
    13261 ggccacgacc caggcattct ggaaatattg atgaaagaaa ggagggtaaa acatgttcga
    13321 cctttcgaaa ttttctcttc agggtaaagt tgccattgtg accgggggcg gccgcggaat
    13381 tggaaaggcc gttgcccagg gttttgccaa ggctggggct aaagtggcca ttaccagccg
    13441 caagatcaac gacctggagg ctactgccgc ggagatcagg ggttttggcg gcgaggcgct
    13501 tccggtccag gctcacttgg ccaaggcaga ggagattagc cgaatggttg gtaccgtgct
    13561 gggcaagttt ggccgggtcg acatcctggt caacaacgcc ggcaccagcc cagctatggg
    13621 ttcggttctg gattcagacg agcgcctctg ggatacggtc atcaacctga acctcaaagg
    13681 gctttactac accagccagg cggttgcccg ggtaatgaag aagcaggggg gcgggagaat
    13741 tatcaatgtg gcctccattg atggattcaa gcccgaaccc ggagttagca tctacagcat
    13801 ttccaaagca ggcgttcgga tggtcaccca ggctttcgcc gctgagttgg ccccttacaa
    13861 catccgggtg aacgccatcg ctcccgggcc gatcagcacc aagatgctgg attctcactg
    13921 gttcaacctt ccccccgaag aggcccaaaa ggccaaagag gctctaggga agttgacccc
    13981 tctgggacgg gttggagaac ccgaggaaat cgccggcgca gccatctacc tggcctccga
    14041 tgcttccagt tataccaccg gggctgagat tgtgattgac ggcggagtcc tcctcgggtt
    14101 cgctgttccg ggggttcagt aaggccgggg aaatacggcc tgagctcttc tgcgacctta
    14161 aaaagggaat ggggggatat gcgcttattc cgggagggtc ggcccaggaa acccagcgtc
    14221 aacctccggc ctttacttgg accccggaag ggttcaaagg gggtggagcc ggtgcgggga
    14281 gggtgggtac caggatactg accctccggt tggagaaatc catcgggttt tccggacgtc
    14341 tcaatctctg gtcggcaaaa ccccggactt cctgaacctg gtccttgcgc aggccgtttt
    14401 ccaccagaat ttttctagcc gtgttggccc ggtccacgct taactcccaa ttggaataat
    14461 tggggcccac atagggtcgg gcgtctgtat agccctccac gatcacccgg ttgggcagct
    14521 tgccgatctc cccagcaatc attcccaaaa gttggagggt ttcctttctg aggtgggcgc
    14581 tgcccacatc aaagaagagg ccggaagagt tgtccaccag ctcgatccgc aggccctcgt
    14641 cggacacggt gatttccacc tgacctttga atttgtcgaa gaccggggtc ttccggatca
    14701 tctcctcgag cttcttcttc tcctcctcca gttgcttcat gtctacgtcc gggtccttgc
    14761 ttaggcggac agccgggggt gggtccgctt gggtgccggg cggggcataa tccggaagga
    14821 tccccgctcc gccgttcttg accttttcgg caaaggcccc agggtcttta aagtattggg
    14881 aaatcatctc cttgatcgtc ttgctttgcc ccacgatcca caagacgatg aagagagcca
    14941 tcatggcggt cacaaaatcg gcgtaggcca ctttccaggc cccgccgtgg tggcccccat
    15001 ggctgctttt cttcttcacg atgatccgag gttgagcttg atctttctcc cgggtcacgc
    15061 cttccctccc cggagggcct gctccatctc cctaaaggtg ggccgggaat catcgaaaat
    15121 ggaccttcga ccgaattcca ccgcaatcat ggggttgaag cccttggaaa aggccaccag
    15181 tccggctttt atcacttcca gcatgcgggc ctcctcctca tgcatgaggt ccaggcttgt
    15241 ggccaggggc tggatgaatc cataagagag gaggatgccc aggaaggttc ccaccagggc
    15301 cgcggctacc ttatggccga cctgctcggg agggccgttg atcgcctgca tggtaatgac
    15361 gattccaagg acggcggcta cgatccccag cccgggcatg gagtctccga ttttctgcag
    15421 gatcatcccc ggcttggttc cttcctgctg ttgggtttcg atgtccgtat ccatgagcgc
    15481 ttcgatttca tagggggaca cgcttcccat gaggatgatc cgcatggtgt cagtcaaaaa
    15541 agagaggaga tgatgctgcc cgacaaactg ggggtatttg ctgaagatgg tgcttttttc
    15601 cgggtgctcg atgtgggatt ccagggccag caccccttcc ttctggctga gctgaaagaa
    15661 ttcatacatc atcttcagga tgccaagata cgtttccttt tcaactttcg atttcttgaa
    15721 aatcaagagc acccggttca ggattttttt taacagcttg gggggagtgg agataagaag
    15781 cgccccggca gccgcccccc cgatgatgat gaactccgca aactgcatga gaaccagaaa
    15841 agacccgccc tcaatcaaat atccgcccaa aaccgctagc aggacaacca gaatcccaac
    15901 gatggccagc attcaatccc cctcaaagag atcgattccc cgtgattcca aaagggcttt
    15961 tccccaaatc ataaccgtaa cgattagata agctgagctt ctctggcgtg cagtcacccc
    16021 tcaagattcg cctgtccggc tcatcccgtc ttaagcgggc aagcccgggc tcagaggaga
    16081 tcgcccactt cgctaatggc atttttgaga agctgaaagg attgaaaatg gataacgatt
    16141 ttcaataatt ttatcggcga attctggtct tttctttaaa cctcgatgct aaaggcgggt
    16201 aaccatgcag atccgggcaa gaggtaaaaa gttttattat atcaaatggt tataacttat
    16261 ttgccaagac tattcgaatc tcttcaccga ggagctagcc aataaaaaga atcgaggaat
    16321 gatggcctgt gagaaatttg aatttcggca taaaccggca ttgttttctg cggcaaaagc
    16381 tcattttatg gctccgtggc cagccaaatt agtgttgacc cggggcgatt ttcgatgtta
    16441 aaataacaaa tgagtcaagc tcagggaggc ggggacatgt ttaaaattgg cgacaaggca
    16501 gtctatcctg cgcatggggt tggagtcatc gaagatattc aatgcaaggt aatcggggga
    16561 tgcaagcgga tgttttatgt cttgaggatc ctggaaaaag atatgaccat catgatcccc
    16621 aaggacaacg tgcagaatgt aggattgcgc gaaatcatcc aaaaaaagga tgttcccaag
    16681 gtgttccgga ttctctcgga gaagaacggg aaagtggatt gccagacctg gaaccgccgt
    16741 taccgggagt acatggagaa gatcaaatcc ggttccgtct ttgaagtggc cgaggtcctg
    16801 cgcgacctct ttcacttgaa gtgcgacaaa gacctttcct ttggggagag gcaaatgctc
    16861 gaccttgccc agaacctcct ggtcagggaa ctcgccagcg ccaaaaacat ttccgaagaa
    16921 aagatgcgcg tccgcctgct gagcatcttc aatgcctgaa gtttctctct ttttttaaaa
    16981 aattcaaaac ggaaaggagg tgaaaagcgg tgggattgct tattctccgg gtcctgcttt
    17041 tcgccgcgac cggggcaagc ggatacttcc tagcccttca ggccttatct ttccccgagg
    17101 ccgggctttg gggattgggg attggatggg ccctggggtt gctggtgatc cttgcggaac
    17161 gaggcatccg gcgggttcct ctcaaaataa ccatcggggg agccatcggc ctggtcttgg
    17221 ggttgatgtt ggccaacgtt ctcatgaact cctttttttc cggaatcctt gaaggactgg
    17281 gcatctattt tcccggctat ttcttgatca acagcgccct tgggtatctg ggcttcctct
    17341 tgggggtaaa gaaggggaaa gaattcgaac tggcccgcct tccctggttc tcccaaaggc
    17401 cgggggaagg gtgtgacggc cacaaagtgc tggataccag tgtgatcatc gatgggcgca
    17461 tcgccgacat ttgcgatacg ggattcatgg aagggacctt catcatcccc caatttatcc
    17521 tccaagaact gcagcacatt gccgattcct ctgaccctct gaagcgcgga aggggacgca
    17581 ggggtttgga tgtgctccac aggattcaga aacaaagcaa tctggaagtc aggatcgtcg
    17641 accaggatta cccgaagatc caggaggtcg acgccaagtt aatcgctctc gccaaagaaa
    17701 tcagcgccaa gattgtgacc aacgacttca acctgaacaa agtcgcggaa ctccagggca
    17761 taccggttct caatatcaac cagcttttta acgctcttaa accggtcgtc ctccccggcg
    17821 aagtaatgaa cgttaaaatc ctgaaagagg gaaaggagcc cgggcaggga gtcgcctatc
    17881 tggacgacgg aaccatggtg gtggtggata atgcccgccg ccacatggga aaaagcgtgg
    17941 acgtcacggt caccagcgtc ctccagacca cagccgggcg gatgattttc accattctga
    18001 aggaagacgc tcccgagaac ttttaccaga gtatcaatca ctgatcgaaa ccgctttcca
    18061 ggcgggaacg gaagaaccca gggtggaaat ccatcctggg ttctctttat agcggtaggg
    18121 acaacctctc gtcccatggc ccgaacaggg atgcctaaat cccgcctcat aagatttgcg
    18181 cataacgggc gggcaggggg gggaaatcga ccttgcgggt agtggccttg atcccggccg
    18241 cgggccaggg aaaaagaatg ggaaccgaaa ggcccaaagc ctttatctcc ctgggcgtcc
    18301 tgcccattct ggcccatacc cttcggaagt ttgaggcctg cccgcaggtg gatgaagtca
    18361 ttcccctggt tccccccggc gagggagtgg cctggacccg ggaaatcgtc tcccaaggtg
    18421 gcctgcaaaa agtgtcccaa ataatgcccg gaggggcgga gcgccaggaa tccgtcttta
    18481 taggattgag ggctatccgg gaaaaggccg aggcggtcat catccatgat ggggtccggc
    18541 cttttgtcac cccggacttg atccagcggg ccctgtcgga ggcccgccgt ttccaggccg
    18601 tagccgttgc cttgccggcc agcgaaacca tcaaggaagt tgcccgggaa aatacagttt
    18661 tgagaaccgt ggaccgccgc caactttgga tgatgcagac ccctcagtct ttcgattatg
    18721 aactgatctg cagggctcat gaagaggccc gcagagacgg attcgtggga acggacgacg
    18781 cctctctggt ggagcgcctg ggtattccgg tcaagatcat cgaagggtcg cggtttaact
    18841 tcaaaatcac cacccccgag gacctggtcc tggcagaggc gctgctgaaa tacagttcgg
    18901 agttcggagt aaaaggattc tcaaatgggg ggctcagcga tttccattag aagatgtaac
    18961 ctcattgaac ccaaagtccc tttcagactt caggcacttt agctcacttc aggcattcta
    19021 ggcaatgtcc ttatgcgtat cggttttggc tacgatgttc atcctctggt agagggacga
    19081 aagctgatcc tgggaggcgt ggagattccc ttcgagaagg ggctggaagg ccactcggat
    19141 gccgacgttc tcctccacgc catatgcgac ggaattctgg gagccctcgc ggaaggggat
    19201 attggaaagc atttccccaa taccgaccct cgattcaagg gcatttccag cctccggctt
    19261 ctagaaggcg tagcttctaa gttggcccaa aagttttttc tcgtggagaa cctggacagt
    19321 acggtggtcg cggaaaaccc caaaatcggg ccttacatcc cccggatggc ggaacaaatc
    19381 gctcgggtgc tccgaatagc tcctcagcag gtcaatatca aggccaccac ctcggagaat
    19441 ctcgggttta tcggagaagg aaaggggatc gcggcctacg cagtggttct cttaaaagaa
    19501 gaatctggca aagtatccag ttaacgctta aagggagccg cggttttgcc ctggggttgc
    19561 ctggcagggg taataaatga gcgcggaaga atggcagcat cacagcattc ctccttttgg
    19621 attggaacgg attctggggt tctatcactc caccctgaac acgaaccttt ggttagcttg
    19681 tggcacttga gcttgcgaaa ttgcgagtaa gcatatttta ggcaatgggt atgaatgttc
    19741 gcgtccgatt tgctcccagc cccaccggcc tgcttcatct gggtaacgcc cggactgctc
    19801 tgttcaattt cctctttgcc cgccagaccg gagggacctt cattctgcgg ctggaggata
    19861 cggaccggga gcgatcgacc cccgaagcgg aaaaagcagt cctggaggac ctgcgctggc
    19921 tgggattgga gtgggatgaa gggccggagc ggccggggga tttcggtcct taccgccaat
    19981 ccgagcgcct ggagacctac cggcgctacg ccatggacct gctggaaaag ggacacgcct
    20041 accgctgtta ttgcaccgat gaggaactgg aggagaagag acaacgctcg ctggccaaag
    20101 gcataccacc catgtatgac ggccgctgcc gctatttgaa accggaggaa gaacagtcct
    20161 tcatccgttc tggccgcccg gcttcggtac gttttcgggt aaacgcccgg agtgcggagt
    20221 tccaggacct ggtcaagggg gccatgtcct ttgacgggcg gaagatgggg gattttgtaa
    20281 tcctccgctc agatcaggtt gcctcctaca attttgccgt ggtcatcgac gacgcccgga
    20341 tggaggtcac ccacgttatc cggggggaag accatctggc caatacggcc cgccaaatcc
    20401 tcctttatca gaccctgggt ttcgcccctc cccaatttgc ccatctctcg atgattttgg
    20461 gaccggatcg gactccccta agcaaacgtc atggggccac tgccgtcgcc cattttcgcg
    20521 aagccggcta cctgcccgag ggatttttga attatctggc tctcctggga tggtcctccg
    20581 aagacggcca ggaaattttc tcactcggag aactcataaa aaagttttcc ctccagcgcc
    20641 tttcccgcag cccggcagtc tttgacccgg aaaaattgaa ttggatgaac cgggctcatt
    20701 tgaaagggat tcatggagaa agagccctca acctggccct ccccttcctc cgccgttccg
    20761 gggaaaaact ggaggcagtc cctaaatcct ggctggtggc ggctttggag gcagtgtggg
    20821 gggaagtgga caccctttct caactggctg agcgcttgag gtttcttttt gacgaggggt
    20881 ggaccctgga gccggatgcc gaaactctgg tggtcaagga agagacccag cgggtgatcc
    20941 gggggttgcg ggaagaactt cgttcggtgg aagaagtggg gccggaaaat taccgggccc
    21001 tcctcgccgg tctggccaaa cgggtcggcc ttaccggtcg aaacctctat atgcccttgc
    21061 gagccgccct taccggaaga acccagggtc ccgaactgga gaaggtattc ctgttgctgg
    21121 gaaaagaaaa gattctgaag cgtttggatt ccatcctgca aagcaatgga aaagagagga
    21181 gcctctagag tgaaaaacca ggatttgatt tccttcatat ccaagttcaa gaatgcgcgg
    21241 atcctggtca tcggggacct catggtcgac gagtacatct gggggagtgt ctcccggatc
    21301 tcccccgagg ccccggtgcc ggtggtcagt gtgacttcgg agagcctgcg tctggggggc
    21361 gcggggaatg tggtgaacaa tatctacacc ctgggaggca aggtctggat cggggggatc
    21421 gtgggaaatg acgagatggg gcggaaagtc atccaggacc tccaccgaat gggggttgac
    21481 acccgggccg tggcggtgga gtctgactgg atcaccacgg tcaagactcg catcatcgct
    21541 catcaccagc aggtggtccg ttacgaccgg gaagtggtcc gaagcatccg tccggaggcc
    21601 ctgaaaaaga tcctttccgt ggtggaggac cgggtccggg agttcgacgc ggttctaatt
    21661 tccgattatg gaaaaggggt catctgcccg gaactggtag agcaggtccg ctctctcgcc
    21721 ctgggagcgg gaaaaatcct gaccgtagac ccgaaagtga agaacttccc cctcttccag
    21781 aaggtgacgg tcatcacccc aaaccaccat gaggccgccc aggctacggg ccggtggatt
    21841 cagaacgaag aggacatggt ttctgtcggc cgccagcttc tcgggcagct tcaggcacaa
    21901 tccgtcctga tcacccgggg agagaagggg atgactctct tccaggagaa tggagaaatt
    21961 accaacattc ccgctatggc taaggaggtc tacgacgtga ccggcgccgg ggacacggtg
    22021 atttcggtcc ttactctggc catgtcggtg ggagccagcg tgaaggaggc cgcagttctc
    22081 tccaaccatg cggccggtat cgtggtggga gaagtgggaa ccgccaccct gaaagcctcg
    22141 gaactcgagg atgccgtcaa acacggcgtc cggagtaaga gcaacccatc ggttcccaac
    22201 gccccaggag ccccgtgagc ttcccccgac ccccgaaagc agttaagttg gcgatgagct
    22261 tcatctgttc ccggggtgag gactttgctt cggccatcgc cgaagccgat cccgaatacg
    22321 ggccagtgga cctggtcagc gagccgcttt ccttccattt taccgattac tatgaaaggg
    22381 aaatgggaaa aggattgtgg cgtaggatgg ttggttttga gcctttgatc gccccggagc
    22441 agctggtctc catcaagctg tgggccaacc gggtggagag tcaaagccgg aacgaacagg
    22501 ggggccggcg ggtcaacatc gacccgggat atgtagccgg gtccaaattc gtcctggcta
    22561 cgggcaaaga ttacagccac cggatttacc tcgggggggg gatctacgga gaaatcactc
    22621 taatcgtacc caaagggggc ctttcccccc tgccctggac ctatccggat tatgcttccc
    22681 aacccctctt gggaatcatc gatctgttgc gcaagagata tctgtggcag atcagacaac
    22741 agggtgaaag gggggaacgg ggaaacggag aaaaaagcta gggcctttcc cccttttcac
    22801 ccctttttgc cctatcattt cagggatgga ttctatgatc aaaagcatga ccggttacgg
    22861 ccggggagaa tggcaaggag atggaaagaa gctgggggta gaaatccggg cggtcaatca
    22921 ccgctattgc gatatctcca tccgcctccc ccggaggcta aatccgctgg aaacccaatt
    22981 acgaaacctg cttcgccagc gtatttcccg ggggcgcatc gaggtttctg ttcaattgga
    23041 cgaatcgtct ctaccggagc agaagctgga acttgacctg cccctggccc gagacatcta
    23101 tctggccttg aaaaaacttc aggaaaatct gggaataccg ggggaagtcc ggctggaaac
    23161 catggccggc ttccgggata tttttggccg aaaggaagcg gagcccaatc tggaaaaaga
    23221 atgggaatgc ctccggacgg ccctggagga tgccctgtcc ggtctggagg ccatgcgcgg
    23281 cgaagaggga cgcaagctcc gggaagactt cctggggaga ctggcggcta tcgaaaaaat
    23341 ggtccagcag atcgaagaga aagctcccct ggctttgcgg gcctgccgcg accgcctggc
    23401 ccagcgggtt caggagctga gcgggggtct ggaaatcgat gagacccggc tggcccagga
    23461 agtgggtttc ctggcggagc ggagcgatat caccgaggag ttggttcggg tccgcagcca
    23521 tctggctcag ttccgggatt tactgggccg cccggagccg acgggccgga aattggaatt
    23581 ccttctccag gaaatcaacc gcgaagcgaa caccatcggc tccaaagcca acgatgccag
    23641 catcgctcac gtgggggtgg aaatcaagag cgaattggaa aaaatgcgtg aacaggttca
    23701 gaacgtggaa taatcattca ccacagaggc acagagaata aagataattg gaagcggatt
    23761 aaaatctggg ctgaaaagtt ttatttttat gaattttttt atctgcgctc tctgcgcctt
    23821 ctgcggtgaa ggtcttttgc tttccaattt tcattttttc tctgtgccct ctgtgtctct
    23881 gtggtgatgc gttgatgaaa aagaacgaag ggatattgtt cgtcgtctcc ggcgcttcgg
    23941 gaacggggaa gtccaccctc tgccgggcta tgttgcggag ctttcccaat cttcgtttct
    24001 ccatttccca caccacccgt ccgcggcgcc ccggggaccg agagggaagg gattacttct
    24061 tcgtccctcc gcaacaattt cagacgatga tcgaccaggg ggagttttta gaatgggccg
    24121 aggtttatgg gcaccgctac ggaacttcga ggcggatgtt ggaaatggct cgcgcagaag
    24181 gtcaggactt gatcctggac atcgacggtc agggagctcg gcagttgcgg gaccagaata
    24241 tccccggcgc attcgtcttt atcctccccc cttccctgga ggagttgaag cggcgtctga
    24301 tgaaccggaa gaccgaagga aaggaagcac tggaggaacg ggtcaaaaaa gcccgggctg
    24361 agattgcgga ttcccattgg tatgactatg tgatcatcaa cgattacttg aaagcggcta
    24421 aggcccgact ccaggccatc atcctggcag agcattgccg gcgagagaag ctcaccgaag
    24481 ctatcgataa attattggtt gaaaagtaga ggagattttt tttaaaattc atagaggtcc
    24541 atagcccctg gctttatcct ccatccccga aagggggcga gggtatttcg gagaagcggc
    24601 cgccccgtgc ccgtctctgc caaagaaggt caaagatctc tatattttgt gccgaaaaat
    24661 tcaatattaa ctcgaggtac ttaagtacac taagcacacc taagacactt ccttttattt
    24721 gcggcatagc cccgctgtgt gctctgtggc tgatgaaagc aaggaggaac ccaaatggct
    24781 cgaatcaccg tggaagattg cttgaagaaa gtttccaacc gttttgccct ggtcctgctc
    24841 gccgtggccc ggaccaagca gctgtataaa gggtccaaac cgcgcgtcca gacggacaac
    24901 aaagaagtgg tcctggcgct gcgggaagtg gccgcgggaa aagtaatgcc ggccaggccc
    24961 ttgcaggaag gtctccaggt gcagggaaaa tcccctaaac agctcaagga gtagcaggag
    25021 gtctggcccc catgaatcca tcttcatccg ccgctggaaa ggtgatcttt gccctcgacg
    25081 ttcccactct ggaagaggcc cgaaaattcg tcagactcct gaaaggacga actggatttt
    25141 tcaaggtagg tctggaactc cacacggcct atggaaaaga agcggtgaaa gcggtgcagg
    25201 aggagggggg aagaatcttt ctggacctga agtttcatga cattcccaac accgtctccc
    25261 gtgccgccga agaggcggtc aagttaggcg tggacatgtt caacctgcat gcttccggcg
    25321 gaacggagat ggtgcgggaa acggtggaac ggtgccggaa gaccg
//
LOCUS       DASPRR010000221         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_368248, whole genome shotgun sequence.
ACCESSION   DASPRR010000221 DASPRR010000000
VERSION     DASPRR010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368248"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VEL68_08635"
     CDS             <1..521
                     /locus_tag="VEL68_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ36070.1"
                     /translation="DALASQGLKPEDIDYVIQTHGAYDHTGNTAKCKNATIFMQEEEW
                     KFAMAPHPVLREIYQPHLLSGWHRLQLVKGDVPNLFPGIDLIFAPGHGPGVQAVSVNT
                     KKGKAVISGFCCVQDNFVAREDMKPYYPVTPSGIHVNPLQAFESMVRVAGLADILLPQ
                     HEPSLLKVKSIP"
     gene            582..1439
                     /gene="panB"
                     /locus_tag="VEL68_08640"
     CDS             582..1439
                     /gene="panB"
                     /locus_tag="VEL68_08640"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXZ36071.1"
                     /translation="MERKKVTPQDLLAKKQQGRKIVRVVCYDYPMALLADRAEVDSIL
                     VGDSVGMVVGGMASTVPVTMEEMIYHCRIVMRAVKYALVIGDLPFLSYQTDKRDAIYN
                     AGLLMKSGGVDAVKLEGGKEFAPTVKAIVEAGIPVVGHIGLTPQTVSKLGGFKVQGTD
                     TAAARKLVEDALSLESAGIFMLTLECVPDRLGEFISKKLKIPVTGIGSGPYTDGQTLN
                     LYDLVGLFEKFTPKFVKKYANLSQEILKALQNYKDEVEKGVFPGSEHSFHMKDEVLKE
                     VIGDPAGKG"
     gene            1742..2599
                     /locus_tag="VEL68_08645"
     CDS             1742..2599
                     /locus_tag="VEL68_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HXZ36072.1"
                     /translation="MNTFFQERVQRKHSQLTRSQLALARYILQHDREVPFLSSAKLAS
                     RVNVSDATVTRFCTALGYSGFSDLQKDLQKWLQMRLAPSERLEKIPRNKGESSYLKIF
                     QRDIQNLRETSAGLQPDQVRKAIGMLTRARRLFIVAMRSSFGLATLAHSHFSGIRSGV
                     TLLESSRGMMPDLLTDVRPSDVMLAISFPRYTRGTLEIAQYVKGRGCRVIAITDSILS
                     PLARVSDLVIQAKIEGLGFINSYTSAVAIINCLSTGVSMANPKMSIRTLKRNELELNK
                     WQIWAVQGS"
     gene            2775..>3034
                     /locus_tag="VEL68_08650"
     CDS             2775..>3034
                     /locus_tag="VEL68_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36073.1"
                     /translation="MRFWFTEEQNKFRQTVRDFLQAELKAGTFVAKSGGLAEGSSQEF
                     SKKMAQRGWIGMTWPKQYGGQGRSYVDKVILNEEIFKVQAPVA"
BASE COUNT          753 a          755 c          844 g          682 t
ORIGIN      
        1 aggatgcgct tgcttcgcag ggactgaagc ctgaagacat cgactatgtc attcaaaccc
       61 acggggctta tgaccatacg gggaatacgg cgaaatgcaa gaacgccacc atttttatgc
      121 aagaagaaga atggaagttc gccatggccc ctcatcccgt tttgcgggaa atttaccagc
      181 ctcatttatt gagcgggtgg catcggctcc agctggtaaa aggagacgtg cccaatcttt
      241 tcccgggaat cgatttgatc tttgctcctg gccatggtcc tggtgttcag gcagtctccg
      301 tgaataccaa aaagggcaag gcggtcatta gcggattctg ctgcgtgcag gacaacttcg
      361 ttgcccgcga agacatgaaa ccttattacc cggtgacccc ttcggggatt catgtcaatc
      421 ctcttcaggc gtttgaaagt atggtcagag tcgccggcct ggccgatatc ttgttacccc
      481 agcacgaacc ttccctgcta aaggtaaaaa gcattccctg atttcggagg gaagccggtc
      541 caaagttaaa atgaacctcg tttttaagaa gggatcctaa aatggagcgc aagaaagtaa
      601 cgcctcaaga ccttctggca aaaaagcagc agggtagaaa gattgtgcgc gttgtatgct
      661 atgactaccc catggcgctg ttggccgatc gtgctgaagt ggattccatc ctggtggggg
      721 attccgtggg aatggtcgtg gggggaatgg cttctacggt ccctgtcacg atggaggaga
      781 tgatctacca ttgccgaatc gtcatgcggg ccgtgaaata cgccctggtg ataggtgatc
      841 ttcctttttt gtcctaccag accgacaaaa gagacgcaat ttacaatgcc ggccttttaa
      901 tgaagagcgg gggggtggat gcagtgaaat tagaaggcgg gaaggaattt gcccccaccg
      961 taaaggcaat tgtggaggca ggcattccag tggtgggcca tattggcctc acccctcaaa
     1021 cggtctccaa gctgggaggg ttcaaggtcc agggcactga tacggcggcg gcccggaaac
     1081 tcgtcgaaga tgccctatcc ctggagtctg cggggatatt catgctgacc ttggaatgtg
     1141 ttcccgaccg tcttggggag ttcatcagca aaaaactgaa gattccagtc acgggaatcg
     1201 gtagcggtcc gtacacggat ggacagacct tgaacctcta tgacctggtt ggcttgttcg
     1261 agaaatttac ccccaaattc gtaaaaaaat acgctaatct ttcccaggag atcttgaagg
     1321 cattgcagaa ttacaaagat gaggtggaaa aaggcgtatt ccccggctcg gaacatagtt
     1381 ttcatatgaa ggacgaggtt ttgaaggaag tgatcggcga tcctgccgga aaaggataaa
     1441 gggataaggt gtttcctgct cggtaaattc gggtacgaat ttgtaccggg ccttcaaggg
     1501 gaaggagacc cgatcacacc aacttttttc ctcgtcatct ccgccacttt cacgcacagc
     1561 atcacccgac atttctgaaa atcttctgac gtattcagat caggtccttc tttccttggc
     1621 cattcgaggg gaccgaatcc ttgggtaata caccagccaa aaaaatcttg acagggaaga
     1681 tccaaaggga taaagtttcc ctacattttc ctcgaattaa tgaaaaattc cctacatcgc
     1741 catgaatact tttttccagg aaagagtcca gcgaaaacat tcccagctaa cccggagtca
     1801 gctggcgctg gcgcggtata ttttgcagca tgaccgggaa gtacccttcc tttcctccgc
     1861 caaattggcc agccgggtga acgtgagcga cgccacggtt acccgattct gcaccgccct
     1921 ggggtactcg ggattttcgg acttgcagaa ggacctgcag aagtggctcc agatgagact
     1981 ggccccttcg gaacgtctgg aaaaaatccc ccgaaacaaa ggggagagtt cttacctgaa
     2041 gatctttcaa cgggacattc agaacctgag ggaaaccagt gctgggcttc agccggacca
     2101 ggtccggaag gcaattggaa tgttgacccg ggcccggagg cttttcatcg tcgccatgcg
     2161 cagctccttc ggattggcga ccttggctca ctcccacttc agcgggatcc ggagtggggt
     2221 gactctgctg gagtcttccc ggggtatgat gcccgacctt ttgaccgacg tcaggccgag
     2281 tgacgtcatg ctggcgatca gctttccccg ttacacccgg gggacgctgg agattgccca
     2341 atacgttaaa ggtagggggt gccgggtgat cgccatcacg gacagtattc tctctccgct
     2401 ggcacgggtc tccgatctgg tcattcaagc aaagattgaa ggcctcggat ttatcaactc
     2461 ctacaccagc gcggtggcga tcatcaactg cctttccacg ggagtatcga tggcgaatcc
     2521 caagatgtcg atccgcacct tgaagaggaa cgaactcgag ctcaacaaat ggcagatctg
     2581 ggcggtccag gggtcttgag gagaattcca ttcttttcgt aatcgccttc tcggtcgtcc
     2641 agagaatagg cgctaccgcc atggcctcct tggaggaaac acggcttcct ccagcagtag
     2701 aaacctggag gttggggaaa ttctaaaagt aatgctgggt gatgatccag cctaagggaa
     2761 aggagacgga gaacatgaga ttctggttta ccgaagaaca gaacaagttt cgtcagacgg
     2821 tccgcgattt tctgcaggcc gaactcaagg cgggaacctt cgtggccaaa agcggagggc
     2881 tggcggaagg aagcagtcaa gagttctcca aaaagatggc ccaacggggc tggatcggga
     2941 tgacctggcc caaacaatac ggaggccagg ggcgctctta cgtggacaag gtgattctga
     3001 acgaggaaat attcaaggtc caggctcctg tggc
//
LOCUS       DASPRR010000222         4632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1342854, whole genome shotgun sequence.
ACCESSION   DASPRR010000222 DASPRR010000000
VERSION     DASPRR010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342854"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1055..1567
                     /locus_tag="VEL68_08655"
     CDS             1055..1567
                     /locus_tag="VEL68_08655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ36074.1"
                     /translation="MEYKIEAMKREDWDEVRAIYEEGIATGNATFEPVVPEWEKWDSA
                     HVAEARLVARVNGQVAAWAALSRVSARKVYAGVAELTIYVGEKFRGKGIGDALLGALV
                     DASEKAGFWTLQGGIFPENTVSIELHKKHGFRVLGIREKIGKMAFGKLQGKWRDVALM
                     ERRSKKAGID"
     gene            1983..2639
                     /locus_tag="VEL68_08660"
     CDS             1983..2639
                     /locus_tag="VEL68_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXZ36075.1"
                     /translation="MEAQVIRRDEAVRQGRRLEYFTVGWNLIEAGVALGAGLFAGSIA
                     LIGFGIDSLIETSSGAVLLWRLKEGEEGEQRERIALKFVGISLLALAAYVAIDAAKGL
                     ILQQPPERSLVGIILAAVSLGVMPLLAWAKRRVAARLSSQALVADSHQTDICAYLSAI
                     LLVGLGLNALFGWWWADPIAALAMVPIIVKDGLEALRGEACEGCSEKGKRGMGVRSGK
                     "
     gene            complement(2628..3590)
                     /locus_tag="VEL68_08665"
     CDS             complement(2628..3590)
                     /locus_tag="VEL68_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005371702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXZ36076.1"
                     /translation="MENGKRKKPSSEKGCSHPPEQRREQLPSSMPKPSEEDPNAPERV
                     RQIMDHPSFRRADQDLDLLKRDELRPIRLQLEFLKPDLILAEHGICGTIVVLGGTRVI
                     EPTAARHRVERARKALEQHPQIPELKHRLAVAERILAKSHYYDVARELGQLVGKAGGG
                     PGDCRLAIVTGGGPGLMEAANRGAYDVGAKSVGLNISLPQEQYPNPYITPELCFQFRY
                     FGLRKMHFHLRAKAMVAFPGGYGTLDELFETLCLIQTRKIKPVPVILVGERFWRHVFD
                     IEFLAAEGTIDPEDAELFVFAETAEEIWKIITDWYAQNGESLFA"
     gene            3898..>4632
                     /locus_tag="VEL68_08670"
     CDS             3898..>4632
                     /locus_tag="VEL68_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXZ36077.1"
                     /translation="MVIPIYQVDAFTEKPFSGNPAAVCLLPQAREAAWMQAVGKEMNL
                     SETAFLLKQEDGYNLRWFTPAVEVDLCGHATLASAHILWETGILRREEQARFHTRSGL
                     LTAVSKDGEIELNFPAKTEEPADPPAGLLEALKVRAKYVGKSKFDYLVQVDSEQTVRD
                     LKPDFTALKHIPVRGVIVTSRAVSPDYDFISRFFAPAAGVNEDPVTGSAHCTLGPFWT
                     RQLGKKEFLAYQASERGGTLRVRVAGD"
BASE COUNT         1012 a         1293 c         1337 g          990 t
ORIGIN      
        1 gctgaaagga attctgctct cttcctttgt cccatcgtcc cttctgaaaa taggtttccc
       61 ctctagcgca tccaatttcg ctcagccagg taccctagcc cagcgactgc tttcccgccc
      121 ccgaaagaag gatagccgca ccagcttttc caaaacacag gggtggatga actcacttac
      181 gcgtagggat cagaactgca agaaaaagcg ggtccgcctc aaatcggcga tggcaaattt
      241 ttcggaatca ccggaagggc gctgaataaa taggcatctg gaacctgccc gctcttgggc
      301 gatcgataaa ttgattgttc atccgaaaaa ctatgattaa aacttcagaa ttatatatat
      361 atgacgggtt cagcgaagtc aagcaatcag aagaacttgc ccacccccag tttttcatgc
      421 ttgttggggt tcagggtgga ggaatttgga tcatcaggct cggcgggtgc ttatccttcc
      481 atgaaactcc tccccctgtt ccgcaggcag aggcctgaga ggagattcct ccaagcagaa
      541 aagagagcca aagcatgttt ccttttcaat ccccccagga caggcccgat ccaatggaag
      601 aaccaccgcc tcaggatgat gatccaaatt tctccgccct gaaccccatg agatcccccc
      661 tcctttccag agatcttatt ttcgaaggac tcttttttaa gaactggggg tgggcaagga
      721 aagaacccat tctccacgga ccctctgcgt tcaagaattc atccctttgt ctccaatcca
      781 ctcgggcgcc cgtttcagct gcccttttct tagacatatt gccatagttg ccggcaatgc
      841 cccgataaaa aatcgttttt ttgatccttc ccggaagacc tccttgaaaa tcttgacttc
      901 ggtagcgatc tgcaatatag ggaaggaaag acggttcgaa atatttaggg gcgaccggca
      961 gttcgactga gctcgccgaa gttcggtggc ccgtgcagtt cgaagtggaa acaaggaccg
     1021 ggaaaatagg aaacgatttt tgcgtggagg caaaatggaa tacaaaattg aagccatgaa
     1081 gcgggaagac tgggatgagg ttcgggctat ctacgaagag gggatcgcca ctgggaacgc
     1141 caccttcgag cccgtggtcc cggagtggga aaaatgggat tcggcgcacg ttgccgaggc
     1201 gcgtctggtt gccagggtga acggccaggt tgcggcttgg gccgccctga gccgggtttc
     1261 cgcccgaaag gtctatgcgg gagtcgccga actgaccatc tatgtgggcg aaaaattcag
     1321 gggaaaagga atcggagatg cgcttctcgg cgccctggtc gatgcttcgg agaaagccgg
     1381 gttctggacc ctccaaggcg ggatcttccc ggaaaacacg gtcagtattg aactgcacaa
     1441 aaagcacggt ttccgggtac tgggcatccg ggaaaaaata ggcaagatgg ccttcgggaa
     1501 actgcaagga aagtggcggg acgtggccct tatggagcgg cggagcaaaa aggcggggat
     1561 cgattagact ccccgcgcgc aacgaatgcc aacgttttgg ccagagggtc tcagaatggg
     1621 gggtggttcg ccaaccgccc ccgcgcgggg ctcccttgca gaagccactc tttcgacgga
     1681 aagtcgcggg aaaagcctcg ggacaaacca ttgcagaagt aatgttataa tgaagtgcgt
     1741 gatttgtcgc atggccggtc aaaattaaaa aatcttgaga attgccaaga aatcgttttt
     1801 ggccgtaccg cctcagaaat ccccccccaa ccccccttta aaaaaggggg gcagaggggg
     1861 atttctggca gggcggcagc gatagggaaa atccgagtta ctggttgggg tcctgtcaat
     1921 cactaggacc ctttggaaag tcattaatca cgtacttttc gaacaaagtg tataattaga
     1981 acatggaagc ccaagtcatc cgcagggatg aagccgtccg gcaggggcgt cggctggaat
     2041 attttaccgt gggctggaac ctgatcgagg caggcgtggc cctcggggca gggctctttg
     2101 ccgggagcat cgccctcatc ggtttcggca tcgattcgct gattgagacc tcttccgggg
     2161 ccgtccttct ctggcggctc aaggaggggg aggaaggcga gcagcgggag cggatagcgc
     2221 tgaagttcgt gggcatcagc ctcctggctc tcgcggccta cgtggccatc gacgcggcca
     2281 aggggctcat ccttcagcag cctccggaac gaagtctggt cgggatcatc ctagctgctg
     2341 tttccctcgg ggtcatgccc ttgctggcat gggccaagcg gagggtagcc gccagactga
     2401 gcagccaggc cttggtggct gattcccatc agactgatat ctgcgcctac ctttccgcga
     2461 tccttctggt agggctgggt ctcaatgctc ttttcggctg gtggtgggct gatccaatcg
     2521 ccgccctggc catggtcccg attatcgtga aagatgggtt ggaagccctg cggggggagg
     2581 catgcgaggg ctgttcggag aagggcaaaa ggggaatggg ggttcgctca ggcaaatagg
     2641 ctctccccgt tctgcgcgta ccaatcggtg ataatcttcc aaatctcttc cgccgtctcg
     2701 gcaaagacga aaagctcggc gtcttccgga tcgatggttc cttctgcggc caggaattca
     2761 atgtcgaaaa cgtgacgcca aaaccgctcc cccaccagga tgaccggtac cggctttatt
     2821 tttcgcgtct gaatcaggca gagcgtctca aagagctcat cgagagtgcc atatcccccg
     2881 gggaaagcaa ccatcgcctt ggcccgaagg tgaaaatgca tttttcgcag gccgaaatac
     2941 cgaaactgga aacaaagctc cggagtgatg tagggattgg gatactgttc ctgggggagc
     3001 gagatgttca atcccacgga tttggccccc acatcgtagg ccccacggtt ggcggcctcc
     3061 ataagccctg gcccgcctcc ggtgacgatc gccagccggc aatccccggg tccacccccg
     3121 gccttcccca ctagctgccc cagttcccgg gctacgtcgt aataatggct cttcgccagg
     3181 atccgctccg ctaccgctag ccggtgtttg agctcgggga tttgagggtg ctgttccagc
     3241 gccttgcggg cccgctcgac tctgtgccgg gcggccgtcg gctcgatgac ccgggtcccc
     3301 cctaggacca cgatggtgcc gcaaatcccg tgctccgcga gaatcaggtc cggcttcaaa
     3361 aattcgagct ggaggcggat gggccggagc tcatcccgtt tgagcaggtc gagatcctga
     3421 tcggcccgcc gaaagctggg gtgatccatg atctggcgga ccctttcggg agcattcggg
     3481 tcctcctcgg agggcttggg catgctcgaa ggcagctgct cccgccgctg ttccggcgga
     3541 tgagagcagc ccttttcaga ggaaggcttt tttcgttttc cgttttccat ctatttcctc
     3601 cgctttctct ccgcgccgca tttggcggcc ggccagaacg aaattcgtca ggacgattcc
     3661 ggtggcatga aggttcagga ttctggttga atctgatttt gggggtttcc aatgattata
     3721 tcgacattgg gccgcgtttt gtaaagccca gattggaata gagagattga agaatgcatc
     3781 cttccgggca aaatgctata atttcgagtg cggcctagcc gcaactaaaa gaaagtgccc
     3841 cccgcctctg gcgggatgat tgcccatcat tggaatggcg gctccggagg gaacaaaatg
     3901 gtcataccga tctaccaggt cgacgctttc acggaaaaac ccttctccgg aaatccggcg
     3961 gcagtctgcc ttcttcccca ggcacgggag gcggcatgga tgcaggccgt gggcaaggag
     4021 atgaatctct cggaaacggc ctttctcctt aaacaggaag acggctacaa cctccgctgg
     4081 ttcacccccg ctgttgaagt agacctgtgc ggccacgcta ccctggccag cgcccacatt
     4141 ttatgggaaa ccggcatttt gcgccgggag gagcaggcca ggtttcacac ccgcagcggg
     4201 ctgctcaccg ccgtttccaa agacggggaa atcgagttga acttcccggc caaaaccgag
     4261 gagcccgccg atcctccggc cggtctcttg gaggccctaa aagtaagggc gaaatatgtg
     4321 ggcaagagta agttcgatta tctcgtccag gtggattcgg agcaaaccgt ccgcgacctc
     4381 aagcctgatt tcactgccct gaaacacatc ccggtccgcg gagtcattgt caccagccgg
     4441 gctgtttccc ctgactatga cttcatctcc cgcttcttcg ctcccgccgc gggagtcaat
     4501 gaagacccgg tcaccggctc ggcccactgc accctgggcc ccttctggac ccgccagctc
     4561 gggaagaaag agttccttgc ctatcaggcc tccgaacgcg gcggaactct ccgagttcgt
     4621 gtggccgggg ac
//
LOCUS       DASPRR010000223         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1342939, whole genome shotgun sequence.
ACCESSION   DASPRR010000223 DASPRR010000000
VERSION     DASPRR010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342939"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1124
                     /gene="rsmI"
                     /locus_tag="VEL68_08675"
     CDS             264..1124
                     /gene="rsmI"
                     /locus_tag="VEL68_08675"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXZ36078.1"
                     /translation="MALQEKPPGTLYVVSTPIGNLEDITLRSLRVLKEVDLIAAEDTR
                     RTRQLLTHYGIHKPLISYHEYNRRMREKTLLQELREGKSIALVTDAGTPGISDPGEEL
                     VRRAIQENIPLVPVPGPAALVAALSVSGLPTESFLFYGFLPSKAVARKRWLTSLRDRP
                     ETLVFYESPRRLGFLLENAAEVLGDRRVVVAREMTKVYEEVYRGTIAEVLDQLGEEQV
                     KGEVTVILEGCTSPPSAEPPSVMEALKHYSQEMGLSMKESVSRVADDLGISRRQVYQE
                     SLRMKKESEG"
     gene            complement(1181..1357)
                     /locus_tag="VEL68_08680"
     CDS             complement(1181..1357)
                     /locus_tag="VEL68_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36079.1"
                     /translation="MAMKKAAKKRAAKGKGKKGEGYTCGVCGLSVTVDEVCGCAESHP
                     IICCMKPMKKKRGR"
     gene            complement(1403..>2322)
                     /locus_tag="VEL68_08685"
     CDS             complement(1403..>2322)
                     /locus_tag="VEL68_08685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ36080.1"
                     /translation="RIGQLVQQKDESQIIADERFQQMLFQGTPYDHPTYGTLESLPKL
                     TIENVKEFYRGSFLAPGSFLVFAGDLSVDRTSRWVEENFPAVSQKKMETREFSASSPR
                     GLRTRIIDRRDLTQSQVRLGHIGIPFAHPGYIPFEVMNYILGGGGFSSRLMQKIRSEL
                     GYTYGIHSFLEPRKFPGPFTISTFTPTDITFSCVQEIGSVLQSFLEQGATVQEREEAV
                     NFFTGSYPRRFETLSQIAQRIIQAESHGVGIEYLSSYPQRIAGVSLEEISRTAREHIH
                     PQDLLAVIVGRAEIFHRAFESLGPVEVLQ"
BASE COUNT          520 a          637 c          659 g          506 t
ORIGIN      
        1 gctgggatgc cgcaacgaag caaaggggat cgtcaatccc ttccatagca gccgtttcga
       61 catcgatgaa gacgtgcttc ctttcggcgt ggaaatgttc gcccggatca tcgacgagta
      121 cctggggttg aaactttttt aaattcggat tcggagtgcg gaatgcggaa taaaataagt
      181 ttccttgttt ccttcaaaag ctggaccccg gacctcgcaa tttcgcattc cacattccgt
      241 attccgcatt tttgatttct ctcatggctt tgcaagagaa acctcccgga actctctatg
      301 tggtctccac tcccatcgga aacctggagg acatcaccct acggtccttg cgggtcctca
      361 aggaagtgga cctaatcgcc gccgaggaca cccggcggac ccgccagctt ctcacccact
      421 acggaatcca taaacccctg atcagctacc acgaatacaa ccgacggatg agggagaaga
      481 ctctcctcca ggaactccgc gagggaaaga gcattgccct agtgaccgat gcgggaaccc
      541 cgggaatctc tgacccagga gaggaactag tgcgcagggc gattcaggaa aacattcctc
      601 tggtacccgt tcccggacct gcggccctgg ttgcggcgct gagcgtctcc ggccttccca
      661 cggagagttt ccttttctac ggatttctcc cctccaaggc tgttgcccgg aaaagatggc
      721 tcacctcact cagggaccgt cccgagaccc tggtgtttta tgaatccccc cggcggctgg
      781 gcttcctgct ggaaaacgcg gctgaggtcc tgggtgaccg gcgggtggtg gtggcgcggg
      841 agatgaccaa ggtctacgag gaggtctatc gaggaacgat tgcagaagtt ttagatcaac
      901 tgggagagga acaagtgaaa ggggaagtta cggtcatcct ggaaggatgc acttctccgc
      961 cctcggccga gcccccctca gtcatggaag ccctgaagca ttattcccag gagatggggc
     1021 tgtccatgaa ggaatccgtt agccgggtgg ctgacgacct gggaatttcc aggcggcagg
     1081 tctatcagga aagcctgagg atgaagaaag aatccgaggg atgactctgc tccccggagg
     1141 agataatcct ccctttgccg gggagcggaa gatcgatcga ttacctgccg cgtttcttct
     1201 tcatgggctt catgcagcag ataatggggt gggattccgc gcatccgcag acctcatcga
     1261 ccgtgaccga caggccgcag actccgcagg tgtaaccctc gccctttttg cccttgcctt
     1321 tggccgctct ttttttggct gccttcttca ttgccatggt tgtaccctcc tttaaggatt
     1381 ttgatggagc gaaccttacg gttcactgga gtacttccac cgggcccagg gattcgaaag
     1441 cccggtggaa aatttcagcc cgacccacga tgaccgcaag caggtcctga ggatggatat
     1501 gctcccgggc cgtccgggag atctcctcca gggagacccc ggcaatccgt tgcgggtaag
     1561 aggacagata ttcgatcccc accccgtgcg attcggcttg gatgatcctt tgagcaatct
     1621 gagaaagggt ctcgaaccgc cggggataac tgccggtgaa gaagttgacg gcttcttccc
     1681 gttcctgaac ggtggcgcct tgctcgagaa aagattggag aaccgagccg atctcttgaa
     1741 cacagctgaa ggtaatgtca gtgggggtga aggtggaaat ggtaaaagga ccaggaaact
     1801 ttcgaggctc caggaaagaa tgaattccgt aagtatatcc cagttcggac cggattttct
     1861 gcatgaggcg ggaagagaat ccgccccctc caagaatgta gttcatgact tcgaatggaa
     1921 tgtaccccgg atgggcaaag gggattccga tgtgccccag gcggacctgg ctctgggtca
     1981 aatcccgacg gtcgatgatt cgggtccgga gaccccgggg tgaggaagct gagaactccc
     2041 gagtctccat cttcttctga cttaccgcag ggaaattttc ttcaacccaa cgggaagtcc
     2101 tgtcgacgga taggtccccc gcgaaaacca ggaaactccc cggggccaaa aaacttcccc
     2161 gataaaattc cttcacattt tcgatggtga gtttgggcaa agattccagg gtcccgtagg
     2221 tggggtgatc ataaggagtc ccctggaaga gcatctgctg gaaacgctca tcggcgataa
     2281 tctgggattc gtccttttgc tggaccagct gcccgatccg gc
//
LOCUS       DASPRR010000224         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1180482, whole genome shotgun sequence.
ACCESSION   DASPRR010000224 DASPRR010000000
VERSION     DASPRR010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180482"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VEL68_08690"
     CDS             <1..224
                     /locus_tag="VEL68_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36081.1"
                     /translation="ILAGGLHPGNILLALEMVSPQAIDVGSGVEARPGKKDPEKVREL
                     FAAVKEFNNLRRSDPPTSIFQKRMEEEYT"
     gene            266..1477
                     /gene="trpB"
                     /locus_tag="VEL68_08695"
     CDS             266..1477
                     /gene="trpB"
                     /locus_tag="VEL68_08695"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXZ36082.1"
                     /translation="MGRNSLPDKRGHFGIFGGRYVGETIMPALIELEKAYAACRADRS
                     FRSDLAYHLREYAGRETPLYLARNLAEKLGGPRIYLKREDLTHTGSHKINNTLGQALL
                     ARRMGKKKVIAETGAGQHGVATATAAALFGMECRVFMGEEDMRRQAPNVSRMEILGAE
                     VIPVTAGTATLKDAMNEALRYWVTHVRDTYYLIGSVAGPHPYPMMVRDFQSIIGRETK
                     RQILQAEGRLPDILIACVGGGSNAMGLFYPFRNHPTVQMIGVEAAGKGMKTKLHAATL
                     CAGSVGVLHGSKTYLLQDGGGQVRDAYSIAAGLDYPGVGPEHSYFKETGRATYVSAGD
                     KEALSAFRLLASCEGIIPAMESAHAIAHAMKLAPTLKRDTVMIVNLSGRGDKDTGIIS
                     DWMRGAEHGAH"
     gene            1464..2258
                     /gene="trpA"
                     /locus_tag="VEL68_08700"
     CDS             1464..2258
                     /gene="trpA"
                     /locus_tag="VEL68_08700"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXZ36083.1"
                     /translation="MERIEKRFSELRKKGEKALVVYLTAGYPDLATSRDLILALDAAG
                     ADVLELGVPFSDPTADGPSIQRASQKALQNGTSLPRILGLVENLRPSTEMPVVLFSYY
                     NPILAYGNDRFARQAGDAGADGVLVVDLPAEEAGELKKHTDPAGLNFISLVAPTTDAG
                     RMRQIVKGASGFIYYISLTGVTGTGRPQVQEIRQNVSRIKKVTQVPVVAGFGVSTPAQ
                     AREIGAETDGIVVGSAFIRLVEEKAEKAGLQKAAYAFARRFKKALR"
BASE COUNT          630 a          744 c          763 g          582 t
ORIGIN      
        1 tcatcctggc cggggggctt caccccggca atatcctttt agcgctagaa atggtttctc
       61 cccaagccat tgacgtggga agcggggtgg aggcgcgacc ggggaaaaag gacccggaaa
      121 aagtgagaga gctgtttgct gcggtgaagg agttcaacaa tctacggaga tcagaccctc
      181 ctacctccat tttccaaaaa aggatggaag aagaatatac atgaagtcat gcctttgcat
      241 taaactaaga aacggaggca gaagaatggg aagaaattct ttgcccgata agagagggca
      301 ttttggaatc ttcgggggaa ggtatgtagg cgaaacgatc atgcctgccc tgattgagct
      361 ggaaaaggcc tacgccgcct gccgcgccga ccgttctttt cggtctgacc tggcctatca
      421 tctccgggag tacgcgggga gggagacgcc cctctacttg gccaggaatc tggccgagaa
      481 gctcggcggc ccccggattt atctcaagag ggaagacctc acccacacag gttcccacaa
      541 gatcaataat accctgggtc aggccctcct cgcgcgtagg atgggcaaaa aaaaggtcat
      601 cgccgagacg ggcgccggcc agcatggggt ggccacggct actgccgcgg cccttttcgg
      661 gatggaatgc cgggtcttca tgggggaaga agacatgcgc cgtcaggcgc ccaatgtctc
      721 ccgaatggag attctaggag ccgaggtcat tcccgtgaca gctggcacgg cgactctaaa
      781 ggatgctatg aacgaggccc tccgctattg ggtgacccat gtgagggata catactacct
      841 catcggctcc gtcgccgggc cccatcccta cccaatgatg gtccgggatt tccagagtat
      901 tatcgggaga gagacaaaga ggcagatttt gcaagccgag ggtcgtcttc ccgacatcct
      961 tatcgcttgc gtgggtgggg gaagcaatgc catgggcctc ttttacccct tccgcaatca
     1021 ccctacggtc cagatgatcg gcgtggaagc agcggggaaa ggaatgaaga ccaaattgca
     1081 cgcggccacc ctctgcgccg gttcggtagg ggtgcttcac ggtagcaaga cctatctcct
     1141 gcaggatgga ggaggacagg ttcgcgatgc ttattcaatc gccgccgggc tcgattaccc
     1201 gggcgtgggc ccagagcaca gctacttcaa ggaaacggga agggccacct acgtctcagc
     1261 gggcgacaaa gaggcgctca gcgctttccg gcttctggct tcttgcgaag gcattatccc
     1321 cgccatggaa agcgctcatg ccattgccca cgccatgaag ctcgccccca ccttgaagag
     1381 ggacacagtc atgattgtca acctctccgg gcgtggggac aaggacacgg gaatcatatc
     1441 cgattggatg agaggtgccg agcatggagc gcattgaaaa gagattttcc gaattgagaa
     1501 agaaggggga aaaggctctg gtggtttacc tcaccgcagg ctatcctgac ctcgccacga
     1561 gccgggactt gatccttgcc cttgacgccg cgggggccga tgtgctggaa ctcggcgttc
     1621 ccttctccga tcccacagct gacggcccct ccatccagag ggcttctcag aaagctttgc
     1681 agaatggaac gtcccttccc cggattctcg gcctcgtgga aaatcttcgg ccctccacgg
     1741 agatgccggt agtcctcttc agttactaca accccattct cgcgtacgga aacgatcggt
     1801 tcgccaggca agcaggcgac gctggagcgg acggagtgct ggtcgtggac cttccggcgg
     1861 aagaggctgg ggagctgaaa aaacataccg acccggcggg ccttaatttc atttctctcg
     1921 tggcccccac aacggacgcg gggcgcatgc gccaaatcgt gaagggggct tcgggcttca
     1981 tttattatat ctccctcacg ggcgtcaccg gtacaggccg accacaggtt caggaaatcc
     2041 ggcagaacgt ttcccgcatc aagaaggtga cccaagttcc ggtggtggcg ggtttcgggg
     2101 tctccacccc agcccaggcg agagaaatcg gcgcggaaac ggacgggatc gtcgtgggaa
     2161 gcgccttcat ccgactggta gaagagaagg ccgaaaaggc tgggctgcaa aaagctgctt
     2221 atgccttcgc tagaagattt aagaaagccc tgagatgata ataatcgaag ggtttttgtt
     2281 ttaacccgtg tttcaaagca agccggagtg ttcagtgttt gatttcagac aaaatatctg
     2341 aaatcggaca gtaggatact tctttcaata aatccgaagt attatcattt ctttcttgtt
     2401 cgtccctgta tgataggaga caattcctcc ctccggaccc ttccgagcgc ccagcggccc
     2461 tcaggttttc tccttagacc taatccttcg atctccaaaa agaatttcct cttcattcgc
     2521 ttcctgcctc ttttctttat ccctctcttc cgggcatggc atcgcctttg ctaaaccaat
     2581 ctcttgtcat gaaggtctta atcgtcgaac aagaaaaaac ctttctgcat tcccttctaa
     2641 attttctcga gcagcagaaa aattttgaag tgtcttgggc ccattcagga aaagaggggc
     2701 tatccctctg gcaggccaa
//
LOCUS       DASPRR010000225         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2148407, whole genome shotgun sequence.
ACCESSION   DASPRR010000225 DASPRR010000000
VERSION     DASPRR010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148407"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /gene="dusB"
                     /locus_tag="VEL68_08705"
     CDS             <1..787
                     /gene="dusB"
                     /locus_tag="VEL68_08705"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXZ36084.1"
                     /translation="PLGVQIFGSNPESMAAAARIAEGCGAGLVDINMGCPVRKVVSGG
                     SGAALLKDLKKAGEIMEAVRKAVSIPLTVKIRTGWDEKTLNFLEVGRIAQENGVDAIT
                     LHGRTRRQGYACKADWESIAQAKKHLRLPVIGNGDLNSPEAVGRFFALTGADGAMIGR
                     GALGNPWIFRQTLQFFQGAFPEPPSLEEKEETILRHLRMIVDSRGEKHGIREFRKHLL
                     WYTRGLRQSSEFRSQVANWVTYPQVVGRTQEYFEKIRKLHLSS"
     gene            925..2025
                     /locus_tag="VEL68_08710"
     CDS             925..2025
                     /locus_tag="VEL68_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HXZ36085.1"
                     /translation="MTIEFRKVWAGPLTQFCQRVFEKLGVPSQDALATTEVLVLADLR
                     GIDSHGVARLRRYYTGLKNGVMVPRPNLRVVRETPVTALIDGGAALGQVAGKRGMEMA
                     IEKALKNGLGFVSVGNSNHYGIAGYYSLMALKHDLIGISLTNSDAYVVPTFGKEVMLG
                     TNPISVAIPTLKERPWVLDMSTAVATLGKVEVYNRMGKKLPHGWATDDKGLSCDDPGR
                     VLSNIRSKTGGGLLPLGGEGEELGGHKGYGLDILIDILSGVLSGSGNTNLLYPKTPEG
                     KPLPSLVGHFFGAMRVDFFRPLEDFKRDMDDMIRRLRNSNKAEGQDRIYIHGEKEYGL
                     EEKYRKEGIPLYYKVYEDLKAIGDEVGVSFNL"
     gene            complement(2094..>2530)
                     /locus_tag="VEL68_08715"
     CDS             complement(2094..>2530)
                     /locus_tag="VEL68_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36086.1"
                     /translation="SMTDLLSRKEGAVLFYRVECFQILARWLDLVAQSLGLAGVEIQE
                     KTLKISSDLFEFLKENKFELIWEKLQQNFRTSRNLWKGFHDWFDGLKTMKLIHHLSAG
                     PYPRKEPETVMPEFLGWAGLEPVEGIGPLLALLRKVQINQVD"
BASE COUNT          592 a          643 c          745 g          550 t
ORIGIN      
        1 gcccctggga gtgcagatct tcggttccaa cccagagtcc atggcagccg cagccaggat
       61 tgcggaaggg tgcggggcgg gactggtgga cattaacatg ggatgcccgg tgaggaaggt
      121 ggtcagcggg ggatcggggg cggctttgct gaaggacctc aagaaggcag gggagatcat
      181 ggaagcggtt cgcaaggccg tctctattcc ccttacggtt aagattcgca ccggatggga
      241 tgaaaaaacc ctcaattttc tcgaagtcgg gaggatcgct caggagaacg gggtggacgc
      301 tattaccctc cacggacgca cccggaggca ggggtatgca tgcaaagctg actgggagtc
      361 catcgcccag gccaaaaagc atctgcgcct cccggtgatc ggcaatgggg acctgaattc
      421 ccccgaggca gtcggccgct tcttcgccct cacgggggcc gatggggcca tgattgggcg
      481 gggagcgctg ggaaatccct ggattttccg acagaccctg cagttcttcc aaggggcttt
      541 tcccgagccg ccctccctgg aagagaaaga agaaaccatc ctccgccatc tccggatgat
      601 cgtcgattcc cgtggtgaaa agcacgggat ccgggagttc cgcaagcatc tcctctggta
      661 cacccggggg ctccggcaaa gctcagagtt ccgctcccag gtagccaact gggtaaccta
      721 tccccaagtt gtcggaagga cccaggagta tttcgaaaaa attcggaaac tccatctttc
      781 ctcttgacat tcctttttat tctccttaga ttctttgccc aagcatcatg attggccaat
      841 aaatttttcc cggcctgggg gccctgaatt tttcagtaga gcccggtctg cgggttgaaa
      901 aaaaaacgga gggggaaggg aagtatgacc attgaattcc gaaaggtttg ggcagggccg
      961 ctgacccaat tctgtcagag ggtcttcgaa aaattgggag ttccttccca ggacgccctt
     1021 gccaccacag aggtcctggt gctggccgat ctgcggggga tcgattccca tggagtagcc
     1081 agattgcggc gctactatac gggattgaag aacggggtga tggtgcccag accgaacctc
     1141 agggtggttc gcgagacccc ggtcacggcc ttgatcgacg ggggggccgc attgggacag
     1201 gtggccggga aacggggcat ggaaatggcc atcgaaaagg cgctgaaaaa tggattgggt
     1261 tttgtctccg tcggcaacag caaccattac gggattgccg ggtattactc gctcatggct
     1321 ttgaagcacg acctcattgg catttccctg accaactcgg atgcctatgt ggttcccact
     1381 ttcggcaaag aagtgatgtt agggacgaac ccgatcagcg tggctatccc aaccttgaag
     1441 gagagaccct gggtgctgga tatgtccact gctgtggcca ccctggggaa ggttgaagtc
     1501 tacaaccgaa tgggcaagaa gcttccccac gggtgggcca cggacgacaa aggcctttcc
     1561 tgcgacgacc caggccgggt cctgagcaat atccggagca agaccggcgg cggactcctg
     1621 cccctgggtg gggaagggga ggaattgggg gggcacaaag gctatggcct ggatatcttg
     1681 attgatatcc tgagcggggt cctctccggg tcgggaaaca ccaacttgct ctaccccaag
     1741 acgcccgagg gaaaaccctt gccttctctc gtgggccatt tcttcggtgc catgagagtc
     1801 gatttcttcc gccctttgga agattttaag agagacatgg acgacatgat ccggcgcctc
     1861 agaaattcga ataaggccga ggggcaggac cggatttaca tccatggcga aaaggaatat
     1921 ggactggaag aaaagtatcg caaagaggga attcccctct actacaaagt ttacgaagac
     1981 ctcaaagcga tcggcgacga agtgggagtg tcctttaacc tatgaatgat gctgatcggt
     2041 catagctcat tgaaaatgat tttggctgtg agcgatgaag tgcgggagat tcctcagtcc
     2101 acttgattaa tttgtacttt ccgcaagagc gcgagtaggg gaccgattcc ttcaaccggc
     2161 tcgagacctg cccagccaag aaattcgggc atgaccgttt caggttcttt gcgggggtag
     2221 ggacctgcgg aaagatgatg gatgagcttc atggtcttta aaccgtcgaa ccagtcgtgg
     2281 aaacctttcc ataaatttcg ggaagtgcgg aaattctgct gcaacttttc ccatatgagt
     2341 tcgaatttat tttctttcaa aaattcaaat agatcggagg aaatctttaa agttttttct
     2401 tgaatttcta cccctgccaa acccaggctt tgagccacca aatccagcca gcgggcaaga
     2461 atttggaagc attccacccg gtagaataag accgcgcctt cttttcggga cagcaagtcg
     2521 gtcatgctgc
//
LOCUS       DASPRR010000226         5904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1343620, whole genome shotgun sequence.
ACCESSION   DASPRR010000226 DASPRR010000000
VERSION     DASPRR010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343620"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..656
                     /locus_tag="VEL68_08720"
     CDS             96..656
                     /locus_tag="VEL68_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36087.1"
                     /translation="MNQPSPSGVPGTPRRGIPRSILLLFFLSCLQICACGETRVKKDL
                     TLARDFRPQEVQNLAVVNLDPQVQFSHFVEAELLKKGYRVKEGSTVGQFLKSEGLLKE
                     GSLDPPALAKIGSRLQVQGIVLCSVMEFSRFRDSYRLSIRCVAPETGNTLWYAEGGKE
                     GRKGQKSSDLLKDIVVSSLKEVPSVR"
     gene            690..878
                     /locus_tag="VEL68_08725"
     CDS             690..878
                     /locus_tag="VEL68_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36088.1"
                     /translation="MFDAEFVCACGFTFKEHFLETEVSRVEDLENFYVRCPRCGEEAS
                     PRSLRLAAGKQSRRRDWS"
     gene            1219..2001
                     /locus_tag="VEL68_08730"
     CDS             1219..2001
                     /locus_tag="VEL68_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ36089.1"
                     /translation="MDKQYVKYVIEEGIAVVTIDNPASLNALNAPTLTQLDQVFDQLA
                     ANLEVKGVIITGGGEKSFVAGADINEFLQVKGDTAAGFMARGQRIFDKIEAFGRPVIA
                     AVNGFALGGGNELAMCCDIRIAAENALFGQPEVNLGVIPGYGGTQRLPRLIGPGKAKE
                     IIFADERITAQEAHRIGLVQRVVPKGQAVEEAKKLLKKIMAKGPVAIQMAKKAINEGL
                     GMSLRGGLDLEGQCEAVCFGSEDKDEGAKAFLEKRPAKFKGK"
     gene            2137..3315
                     /locus_tag="VEL68_08735"
     CDS             2137..3315
                     /locus_tag="VEL68_08735"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXZ36090.1"
                     /translation="MQEVYIMSGARTAIGDFGGALKDFLGHQLVVHPMEEAIKRAKIR
                     KEMVEEVILGHCIQRTDEPNTARTAAIKMGLPMSVPAFTVQRQCSSAMQAIASGAQMI
                     KLGEADVVMAGGVETMSSAPYLLKTARWGQRLQHGQMADTVWDVLTDPLNGMLMGAAT
                     ELLAEKYKITREEMDEVAYRSHKNASRAMAEGRFKDEIVPVPIAQRKGPPKIFDTDEH
                     PRKDLSMEDLTKLPSVFKKGGTVTAGNSSGINDGASAVVLMSGEKAQKLGLKPMARIV
                     SYAWAALEPEYFGYGPVPATQKALQRANLTLGDMELIEVNEAFAGQYMACEKGLGLKR
                     EIVNVNGSGIALGHPVGSTGARIVITLLYEMARRGLKMGLATLCVGGGMGMSMIVERM
                     "
     gene            3428..4279
                     /locus_tag="VEL68_08740"
     CDS             3428..4279
                     /locus_tag="VEL68_08740"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXZ36091.1"
                     /translation="MKKICVLGAGLMGSGIAQVAAEAGYEVAMRDIEDRFVQGGLSYI
                     KKNYERNISKGKMTKEQADGILARVKGLVDLGTAVKGAQVVIEAVVENMDLKRQVYKE
                     LDQLTERETILASNTSGLSITEIASATKKPAKVIGMHFFNPVPVMKLVEVIKGQMTSE
                     DTYGIIKGLAEKMGKTPIAVNEAPGFAVNRILVPMINEAIFVVMEGVASAEDIDKGMM
                     LGANHPIGPLALADMVGLDTLLSVQENLYKELGDTKYRPAPLLRKMVRAGHFGRKTGK
                     GFYNYAK"
     gene            4351..4710
                     /locus_tag="VEL68_08745"
     CDS             4351..4710
                     /locus_tag="VEL68_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36092.1"
                     /translation="MKPAELSAEEILAGKAVQKIRTWLEKETENRGGDFTTRLAIKFC
                     GGCNPLLEREELAAKVRRGLSASQWVSWEEDSDLVLIINGCPTACADRAQTQKNSRAC
                     LVIQPGGVSDIEKMHRA"
     gene            4756..>5904
                     /locus_tag="VEL68_08750"
     CDS             4756..>5904
                     /locus_tag="VEL68_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXZ36093.1"
                     /translation="MEDNKLKEIIKARESWEKDCYQPSLSQSPERLPRFSTVSDLEIK
                     KLYSPEDIARMNFLRDIGFPGQYPYTRGVQASMYRGRVWTMRMFSGFGSAEDTNQRYK
                     YLLSQGQTGLSVAFHYPTLMGYDSDSPRSKGEVGQCGVAIDTLKDMEILFAGIPLDKV
                     TTSMTINPPAAILLAMYIAVAEKQGVSKKEIGGTIQNDMLKEFIAQKTFMLPPRPSVR
                     LIVDTVEYCTQEVPKWNTISISGYHIREAGSTAVQELAFTLADGIGYVQACVERGLKV
                     DDFAGRLSFFFNSHIDFFEEIAKYRAARRLWARIMRERFKARNPRSWMLRFHTQTAGC
                     SLTAQQPYNNVIRTTVEALAAVLGGTQSLHTNSLDEVYAVPTAEAATIA"
BASE COUNT         1448 a         1522 c         1748 g         1186 t
ORIGIN      
        1 ccacggattt cattcccgcg aatggcggaa gggagggggg caggagattt ttcctgcccc
       61 cctgaatgct tttggggaga ggagaaatcc attccatgaa tcagccctct ccatccggag
      121 ttccgggcac cccccgaagg ggaattcccc gatccattct gctcctcttt tttctttcct
      181 gcctacagat ttgtgcctgt ggtgagactc gggtcaaaaa ggacctgacc ttggcccggg
      241 atttccggcc gcaggaagtc caaaacctcg ccgtcgttaa cttagacccc caggttcaat
      301 tctcccattt tgtggaagca gaactgctga agaaaggata tcgggtaaaa gagggttcaa
      361 ctgtaggaca gttcttgaag agtgagggcc tgctcaaaga aggttcattg gaccccccgg
      421 ccttggccaa gattggaagc cgccttcagg tccagggaat tgttctctgc agcgttatgg
      481 aattcagccg gttccgggat tcctaccgac tgagcatccg ttgcgtggcc cccgaaactg
      541 ggaataccct ttggtatgcc gaagggggaa aagaagggcg gaaggggcag aaaagcagcg
      601 atttgctgaa agacattgtc gtctcttctt taaaggaggt tccttcggta cgataggtcg
      661 cccatccggt ctttctgcga gccatccaca tgtttgatgc cgaatttgtc tgcgcctgcg
      721 gatttacatt taaagaacac tttctggaga cagaggtttc ccgggttgaa gacctggaaa
      781 acttctatgt ccgctgcccc cgctgcggcg aagaagcctc cccccgctcc ctgcgactgg
      841 ccgccggaaa gcaatctcgc aggcgggatt ggtcctgaga aaaagactct ccgctattta
      901 tccgcggatt acgcagaggg taatgagata taaatgttac gaatcaaaaa aattttaccc
      961 tgatcaaagt tgagatcagc ttctccggcc gttccctttt gccattcgag ttacacccgc
     1021 gtggcctgcg ttcatccgca tcctgtcagg cttttttatt ccgcattcgt gaaacccctt
     1081 gacataactt ttccagattt gtaatgatca atcaacccgc gataccttcg cgtaagatgc
     1141 ccgaagaaag aatttctcaa tgaaaggact ggtttcatcc gaaatttctg aattcaatca
     1201 cctagagagg aggcttgtat ggacaagcaa tacgtaaagt atgtcataga agagggaatc
     1261 gctgttgtga ccattgacaa tcccgcatcg ctcaatgccc tgaatgcccc gaccctgacc
     1321 cagttggatc aggtttttga ccagctggcc gccaacctgg aggtgaaagg ggtgatcatc
     1381 actggaggtg gggagaaatc cttcgtagct ggagccgata tcaatgagtt cctccaggtg
     1441 aaaggggaca cggctgccgg gttcatggcc cgggggcaaa ggatttttga caaaatcgag
     1501 gctttcggtc ggccggtaat tgcggcggtc aacgggtttg ccctgggggg agggaacgag
     1561 ctggccatgt gctgcgacat ccggattgcg gcagaaaacg cgctcttcgg ccagcccgaa
     1621 gtaaacctgg gagtcatccc ggggtacgga ggcactcagc gccttccccg tctgatcggt
     1681 ccgggcaaag ccaaggaaat catctttgcc gacgagcgaa tcactgccca ggaagcccat
     1741 cggattggcc tggttcagcg ggtggttccc aaagggcagg ccgtggagga ggcgaaaaag
     1801 ctcctgaaga agatcatggc caaagggccg gtggccatcc agatggccaa aaaagcgatc
     1861 aatgaggggc tcggcatgtc cctacgggga ggtttggacc tggaaggcca gtgcgaggca
     1921 gtctgcttcg gcagcgaaga taaggacgag ggggccaagg cttttctgga gaagagaccg
     1981 gccaagttta aaggaaaatg aaaaagacag tggcagtgtc agtgaatagt gtcagtaaag
     2041 gctttgacct aagggccaat gaaattagac taaatattgc cgttactgat actgacacta
     2101 aacactgaca ctaaaaaaat ggaggattcc atgtccattc aggaagttta catcatgagc
     2161 ggtgcccgta ccgcgatcgg ggatttcggg ggcgccttaa aagatttttt gggacaccag
     2221 ctggtggtgc accccatgga agaggccatc aaacgggcga aaattaggaa agagatggtg
     2281 gaggaggtga tccttgggca ttgcatccag cgaaccgatg agcccaacac ggcccgtacg
     2341 gcggctatca agatgggcct gcccatgagt gtgccggcct ttaccgtcca gcggcagtgc
     2401 agctcggcca tgcaggccat cgcttcgggc gcccagatga tcaagctggg agaagccgac
     2461 gtagtcatgg caggaggggt ggagactatg tcttctgctc cctacttgct caagaccgct
     2521 cgctgggggc agcggctgca gcatgggcag atggccgaca cggtgtggga tgtgttgacc
     2581 gatcccctga acgggatgct catgggcgcg gccaccgagc tcctggcgga gaaatacaag
     2641 atcacccggg aggagatgga cgaagtcgcc tatcgaagcc ataagaacgc cagcagggcc
     2701 atggcggaag gaagattcaa agacgagatc gttcccgtcc ccattgccca gagaaaaggc
     2761 ccacctaaga ttttcgatac cgatgaacat ccccggaaag acctctccat ggaagacctg
     2821 accaagctcc cctcggtctt caaaaaaggg gggacggtga cggcgggtaa ctcctcaggg
     2881 atcaacgacg gggcttcggc cgtggtgctt atgtccggag agaaggcgca gaaactcggc
     2941 ctgaagccca tggcccgcat cgtctcttac gcctgggctg cactggagcc ggaatacttc
     3001 ggatacggcc cggtcccggc cactcagaaa gccctgcagc gtgcgaacct gacccttggg
     3061 gacatggagc tgatcgaggt gaacgaagcc ttcgccggcc agtacatggc ctgcgaaaag
     3121 gggctgggtc tgaagagaga aatcgtgaat gtgaacggaa gcgggattgc cctgggtcat
     3181 ccggtgggtt ctactggagc ccggatcgtc ataaccctgc tttacgaaat ggcccgcagg
     3241 gggttaaaga tggggttggc cacgctgtgc gtgggtggcg gtatgggcat gagcatgatc
     3301 gtggaaagga tgtagacaag tttcgggctt tcgttctgat tttttggtca cgatcaaagg
     3361 tttatatccg tattttctgc gtaaatctgc gtccaataaa aattgataaa aggagaggac
     3421 gatggagatc aagaagatat gcgtattggg tgcgggactg atgggttcgg ggatcgccca
     3481 ggtggctgcg gaggcaggat atgaagtggc gatgagggat attgaagacc ggtttgtcca
     3541 gggcggtctt agttacatca agaagaacta cgagcggaac atcagcaagg ggaagatgac
     3601 taaagagcaa gcagatggaa ttctggctcg ggtcaaggga ttggtcgacc tgggaacggc
     3661 ggtcaaaggc gcccaggtgg tcatcgaggc cgtggtggaa aacatggacc tgaagagaca
     3721 ggtgtacaaa gaactggacc agctcaccga aagggaaacc attctggcct ccaacacctc
     3781 gggactctca atcaccgaga ttgcctctgc caccaaaaaa ccggccaagg tcatcggcat
     3841 gcacttcttc aacccggtcc cggtgatgaa gctagtggag gttatcaaag ggcaaatgac
     3901 ttccgaggat acctacggga tcatcaaagg cctggccgag aagatgggaa agacgcccat
     3961 cgcggtgaat gaagcccccg ggtttgctgt caaccgaatc ctggtgccca tgatcaacga
     4021 ggccatcttc gtcgttatgg aaggtgttgc ctcagccgaa gacatcgaca aagggatgat
     4081 gctgggagcc aatcatccca tcgggccgct ggctttggct gacatggtcg gtctcgatac
     4141 cctgctgagt gtccaagaaa atctctacaa agagctggga gatactaagt accgtccggc
     4201 cccactcctc aggaaaatgg tccgggccgg ccatttcggt aggaagaccg gcaaagggtt
     4261 ctacaactac gccaagtgag aaaggcatag agcatagcgc aaagagcaaa gataaagaac
     4321 cgccacttcc ctattccagg ggatgggggg gtgaagcctg cggaattaag cgccgaagaa
     4381 atactggccg ggaaagcagt tcaaaaaatc cggacttggc tcgaaaagga aaccgagaac
     4441 aggggaggag actttactac ccggctggca atcaaattct gcggcggctg caacccgctt
     4501 ctcgaaagag aggagctggc cgcgaaggtc cggcgaggat tgtcggcctc tcagtgggtt
     4561 tcctgggagg aggactcaga cctggtcctg atcatcaatg ggtgcccgac ggcttgcgct
     4621 gaccgggcgc aaactcagaa aaattcaagg gcctgcctgg tgattcaacc gggaggcgtg
     4681 tctgacattg aaaaaatgca ccgcgcatag gtcagagggt gaaaaaaatg agcaggcggc
     4741 agatcggagg aagacgtgga ggataataaa ctcaaggaga tcatcaaggc aagagaatcc
     4801 tgggaaaagg actgctacca gccttccttg agccagagtc cggagcggtt gccaaggttt
     4861 tccacggtct cagacttgga aatcaagaag ctttattccc ccgaagatat tgcccgtatg
     4921 aatttcctgc gggacatcgg atttcccggc cagtaccctt acacccgcgg ggttcaagcc
     4981 tcgatgtacc gcgggagggt ctggaccatg cgtatgttct ccggcttcgg aagcgcggaa
     5041 gataccaacc agcggtacaa gtacctcctc tcccagggac agacgggcct atccgtcgct
     5101 ttccactacc ccaccctgat ggggtatgat tcggacagcc ctaggtcaaa aggggaagtg
     5161 gggcagtgcg gcgtggccat cgacaccctg aaagacatgg aaattctttt cgctggaatc
     5221 cccctggaca aagtaaccac ctccatgacc atcaatcccc cggcggcgat cctcctggcc
     5281 atgtatatcg ccgtggccga gaaacagggg gtcagcaaaa aggagatcgg gggtacgatc
     5341 cagaacgaca tgctcaagga attcatcgcc cagaagacct tcatgctccc gccccggcct
     5401 tccgtgcggc tgatcgtcga caccgtggag tactgcaccc aggaagtccc caaatggaat
     5461 accattagca tcagcggcta ccacattcgg gaggcgggct cgaccgccgt gcaagagctt
     5521 gccttcacct tggcggatgg gatcggctac gtccaggcct gcgtcgaaag aggcctgaag
     5581 gtggatgatt tcgccgggcg tctctctttt ttcttcaact cgcacatcga ctttttcgaa
     5641 gaaatcgcca agtaccgggc cgcccggagg ctgtgggcca ggatcatgcg ggaacggttc
     5701 aaagccagga acccccggtc ctggatgctc cgcttccaca cgcagacggc gggctgttcc
     5761 cttaccgccc aacagccata caacaacgtt attcggacca ccgtagaggc cttggcagct
     5821 gttttgggcg gaacgcagtc gctccacacc aattcactgg acgaagttta tgccgttccc
     5881 acggcggagg cagccaccat tgcc
//
LOCUS       DASPRR010000227         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_57569, whole genome shotgun sequence.
ACCESSION   DASPRR010000227 DASPRR010000000
VERSION     DASPRR010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57569"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VEL68_08755"
     CDS             <1..366
                     /locus_tag="VEL68_08755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698797.1"
                     /note="NuoBCD; NDH-1 shuttles electrons from NADH, via FMN
                     and iron-sulfur (Fe-S) centers, to quinones in the
                     respiratory chain; subunits NuoCD, E, F, and G constitute
                     the peripheral sector of the complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B/C/D"
                     /protein_id="HXZ36094.1"
                     /translation="ILEQVAAQMPAGRHITDDYRYVVPDKADTLKVIESLIHHFIHVT
                     RGPKIPRGEAYASTEAPRGEQSYYVVSDGLSTAYRMRIRTPDFANVQVLPLMAVGGTI
                     SDLVAIAGSVDYILPDIDR"
     gene            420..917
                     /gene="nuoE"
                     /locus_tag="VEL68_08760"
     CDS             420..917
                     /gene="nuoE"
                     /locus_tag="VEL68_08760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HXZ36095.1"
                     /translation="MLPDELRADLQKKIVEAQHPREMAVDVMVALQNFYGYLSDEALE
                     EGAALLGMTPLEMEELATFYDFIYRQPVGRYVLHVCDGIVCWVNGYGGIRDYLCEKLG
                     IRVGETTPDGMFTILPTACLGYCDLSPAMLVNRRPYGPLNPEKVNSILEELRRQPPPR
                     AARIK"
     gene            1004..>2062
                     /locus_tag="VEL68_08765"
     CDS             1004..>2062
                     /locus_tag="VEL68_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HXZ36096.1"
                     /translation="MNRPYKRYKKPMYPQVLLQNRKPDRIATLDEYRKSGGYEALADV
                     LKKKSPKEVQQIVLDANLLGRGGAGFPAGKKWMAVADHAPFPRYLVTNIDEMEPGTFK
                     DRVMAHADPHMIIEGTIIAAYAVSAQKAFLFIRPSYETSALIFERELRVAREAGFLGK
                     NILGSGFSLEAVVHRSGGRYICGEGTAQLNAIEGKRPNPRGVPPHPTDSGLWSLPTVL
                     NNMETVACVPHILRHGAEWFRNLGKTKEAAGTKLYCVSGKVRNPGCYELPMGTPLNEI
                     IEDHGGGMLSGSEYRTCLPGGASTRFLPKEHSNIMMDYPPLKAIGNRLGTGGVIVFDR
                     KTCLVGAALNLTEFFVRES"
BASE COUNT          430 a          615 c          613 g          404 t
ORIGIN      
        1 attctcgaac aggttgcggc ccagatgccc gccggcagac acatcacgga cgactaccgg
       61 tacgtggtgc cggacaaggc cgacaccctg aaggtcattg aaagcctgat ccaccacttt
      121 atccacgtca cccggggccc caagattccc aggggagagg cgtatgcttc gacggaagcc
      181 cctcgggggg aacaatctta ttatgtagtc agtgacggcc tgagcacggc ctaccggatg
      241 cgcatccgga ctcccgactt tgccaacgtg caagtccttc ctctcatggc cgtggggggt
      301 acgatctccg acctggtggc tatcgccggc tccgtcgatt atatactgcc ggatatagac
      361 cgataaaaat atagtttcgg gtttcgagtt tcgggttggc gtcagagctg ggattaatta
      421 tgttgccgga cgaattacgg gctgacctgc aaaaaaagat tgttgaggcg cagcatcccc
      481 gggagatggc tgtggatgtt atggtggcgt tgcagaattt ctatggatat ctttccgatg
      541 aagccctgga ggagggggcc gctctcctgg ggatgactcc gctggagatg gaagagctcg
      601 ccaccttcta cgacttcatc taccgccagc cagtcggccg ttatgttctc cacgtttgcg
      661 acggcattgt ctgctgggtg aatgggtatg gggggatccg cgactacctg tgcgaaaaat
      721 taggcatccg ggtgggagag acgaccccgg acgggatgtt caccatcctg cccaccgctt
      781 gcctggggta ttgcgacctg tctccggcca tgctggtgaa ccgcaggccc tacggccctc
      841 tcaatccgga gaaggttaat tccattctgg aggaactgag gagacaacct cctccgcggg
      901 cagcgcggat caagtagggt gcgttccccg aacgcaccgt ttggcggcct gatcggggat
      961 caggtcccac attatcgtaa ccaagcatcc gaaggggcga ttcatgaatc gcccctacaa
     1021 aaggtataag aaaccgatgt atccccaggt gctcctccag aatcgaaaac ccgaccggat
     1081 cgccactctg gatgaatacc ggaagagcgg cggttacgag gccctggccg atgtcctgaa
     1141 aaaaaagtcc cccaaggaag tgcagcagat cgtccttgac gccaacctcc tggggcgggg
     1201 gggcgcaggc ttcccggccg ggaaaaagtg gatggccgtg gccgaccatg ctcccttccc
     1261 ccgttatctg gtcaccaata tcgacgagat ggagccgggc accttcaagg accgagtcat
     1321 ggcccacgcc gacccccaca tgatcataga ggggacaatc atcgccgctt atgccgtctc
     1381 ggcccaaaag gctttcctct tcatccgccc ctcctacgaa accagcgccc tgatcttcga
     1441 acgcgagctc cgggtcgccc gggaagccgg gtttctgggc aagaacattc tgggaagcgg
     1501 cttttccctg gaagcggttg tccaccggag cgggggccgc tacatttgcg gtgagggcac
     1561 cgcccagttg aacgccatcg agggaaagag gccgaatccc cggggggtcc ccccccaccc
     1621 tacggatagc ggcctttggt ccctccccac ggtcctgaac aacatggaga ccgtcgcctg
     1681 cgttccccat attcttcggc atggggccga atggttccgc aacctcggca agaccaaaga
     1741 agcagcaggg accaagctct actgcgtgag cggcaaggtg agaaatcccg gatgctacga
     1801 gctccccatg ggaaccccgc tgaacgagat catcgaagac cacgggggcg gcatgctttc
     1861 cggttcggag taccggacct gcttgcccgg aggggcttcg acccgtttcc tgccgaagga
     1921 gcattccaac attatgatgg attaccctcc tctgaaggcg atcggcaacc gccttggcac
     1981 ggggggggtg atcgtcttcg accgcaagac ctgtctggtg ggagcggccc tgaatttgac
     2041 cgaattcttc gtccgcgagt cc
//
LOCUS       DASPRR010000228        22165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_206873, whole genome shotgun sequence.
ACCESSION   DASPRR010000228 DASPRR010000000
VERSION     DASPRR010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206873"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VEL68_08770"
     CDS             <1..936
                     /locus_tag="VEL68_08770"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXZ36097.1"
                     /translation="FPAGSVKLLASERSTGKELGFNGKPVKVEKLSKDSFKGIQIALF
                     SAGASRSLEFAPAAAASGAVVVDNSSAFRMDPEIPLVVPEVNPHAVAQYKKRGIIANP
                     NCSTIQMVVALKPIHDAARIQRIVVSTYQAVSGTGLKAIDELLVQTRALLASQEVQKK
                     VYPHQIAFNCLPHIDVFLENGYTKEEMKMVNETRKIMEDPTIRVTATTVRVPVVHSHS
                     ESSNIETEKKITPQQVREILSKAPGVKVVDNPSLNEYPLAIHAAGRDETFVGRIRADE
                     SIPNGINMWVVSDNVRKGAALNAVQIAEILIEKYI"
     gene            1149..1955
                     /locus_tag="VEL68_08775"
     CDS             1149..1955
                     /locus_tag="VEL68_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HXZ36098.1"
                     /translation="MKFLQDITLGQYLHGNSFLHRLDPRTKFLSLILLMVVTFTVQDF
                     LPMVLLWFLFFLGLAVSGLPWAYVFRGVRSFLWLFLFTACLHFFFTPGPSLPFFPIGF
                     LDLTWTGLAKGVFVAAQLFLAILLSSLMTLTTSPLQLAHGLEKLLAPLKRFRVPVEDF
                     SMMTMLAIKFIPILLGEANRIIKAQSARGVDFESGSFFRRVKNLLSILTPLFHSVFKR
                     ADDLAIALLARGYLSGVKRTHLHEPQMTGKDYWVLACVVGFMVFELELSG"
     gene            2046..2582
                     /locus_tag="VEL68_08780"
     CDS             2046..2582
                     /locus_tag="VEL68_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXZ36099.1"
                     /translation="MRKKKFNGLIQVYTGNGKGKTTAALGLALRAAGHRFRVLMVQFL
                     KGGIAYGELKSARKLSPYLTIVPMGRECFVDKKNPHSVDRRWARKGWHLSKEAVQSQK
                     YQVVILDEVNVAVDYGLVPLDELLHLMKNKPENVELVLTGRWATPEVLRQADLVTEMK
                     EIKHYYRKGIESRIGIER"
     gene            2735..3292
                     /gene="rsmD"
                     /locus_tag="VEL68_08785"
     CDS             2735..3292
                     /gene="rsmD"
                     /locus_tag="VEL68_08785"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXZ36100.1"
                     /translation="MRVISGKCKGKKLFALKGLSLRPTSDRVKEAIFDILQKFPPKKN
                     VLDLFAGTGALGIEALSRGAQRAVFVEGSARSGAVLRRNIEACRLSGQAEVVSKEVQT
                     GLKALEEREDSFDLIFLDPPYGKGLACRALESLSRSHILSVNALIVAEHSPDEDLSSI
                     SSLERIDRRKYGGTEVSLFRRKEKE"
     gene            3420..3920
                     /gene="coaD"
                     /locus_tag="VEL68_08790"
     CDS             3420..3920
                     /gene="coaD"
                     /locus_tag="VEL68_08790"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXZ36101.1"
                     /translation="MSERIAVYPGSFDPITNGHVNLIERALQVFDKLIVAVAHNPNKT
                     ALFSVEERIDMIRIALKNDPRVSTASFQGLLVEFAVQQGANVIVRGLRALSDFEYEFQ
                     MTLMNRKLNRKVDTMFLMTGYKWFYTSSKIIKEAASLGGSVRGLVPEIVHQRLKEKFP
                     AYRNLP"
     gene            4021..5214
                     /locus_tag="VEL68_08795"
     CDS             4021..5214
                     /locus_tag="VEL68_08795"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXZ36102.1"
                     /translation="MKLAERVNRIKPSPTFAAAAKAAAMRAKGIEVISFAQGEPDFDT
                     PDNIKEAAYKALREGITKYTPASGFPDLKDAILQKFKRDNGLTYDRSQVFASAGAKLI
                     IYNLAQALLQAGDEVIVPAPYWVSYTDIVLLMDATPVVVQTSEESGFKMSVSQLKAAL
                     TPRTKAIILNSPCNPTGATYTAAELKGLVELLLPRKILIVADDIYEKFVYDGTVFTSI
                     ASLSKEVQDQTIVINGVSKTYSMTGWRIGYAAGPADLMAAMDKVQTQNVSNPVSFCQK
                     AAVEALTGPQDSVQKMVTEFDRRRKYIVQRLNAMPGVKCAMPKGAFYVFPNITGLFGK
                     KWGDKVISNSGDVTDLILEEAKVAVVAGSGFGNDRHIRFSYATSMANIEKGMDRIQEA
                     LRKLK"
     gene            complement(5240..5719)
                     /locus_tag="VEL68_08800"
     CDS             complement(5240..5719)
                     /locus_tag="VEL68_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXZ36103.1"
                     /translation="MGTKKIICPQCGAEVFVYSNPFPTVDIIIEVQPDGGKRGIVLIY
                     RKNEPRAWALPGGFVDYGETLENAAAREAREETGLRIESLEQFHTYSDPQRDPRQHNI
                     STVFIAKAKGMARAGDDAGKIGIFSENNLPLPLAFDHEQILRDYFEKKRSAFSSRKC"
     gene            5913..6377
                     /locus_tag="VEL68_08805"
     CDS             5913..6377
                     /locus_tag="VEL68_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36104.1"
                     /translation="MKRFEFLISPIVLFLFLGLVAGCAISKKEEGKPPSDTSLKASFS
                     DFEGVVIPSELSLNKKKSYVYSAARTKVGLLTFEGGVEPDSLATFFQTNLPRGGWKPM
                     TVLKDREHSLIFLKDDRVCLITISETWFTTICEVRVGLVEKTPEPSKETPTR"
     gene            6402..7595
                     /gene="coaBC"
                     /locus_tag="VEL68_08810"
     CDS             6402..7595
                     /gene="coaBC"
                     /locus_tag="VEL68_08810"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXZ36105.1"
                     /translation="MLKGKKIVLGVTGGIAAYKAAELVRELVRSGAEVFVVMTRSAQE
                     FITPLTLQTLSGNKVATELFSLLEESEIGHISLADRADILVIAPATANMIGKIAGGIA
                     DDMLSTVVMATQAPVLLAPAMNVHMWENSITQENIQKLRARGYHFIDPEAGELACGYE
                     GKGRLAEIPAIVEEVQTLLSPKDYSGETILVTAGPTEEPIDPVRFLSNRSSGKMGFAV
                     SRAARRRGAQVTLVSGPTALTPPPHLRFIPVRTAAQMREAVLENLQAASIVVMAAAVS
                     DFRPQGTREEKIKKSKANLNLLLELNPDILYEAGQKKGTRLLIGFAAETHDLLQNARQ
                     KLAEKNLDLIVANDVSLPGAGFAADTNIVKLIDRRGNIEELPLMGKEEVADLILDRLV
                     KLRTQ"
     gene            7592..8341
                     /locus_tag="VEL68_08815"
     CDS             7592..8341
                     /locus_tag="VEL68_08815"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXZ36106.1"
                     /translation="MNAKPSSLGLDHPRFLVELRELLVGLRHHLEEEKSLGLEGWPRS
                     SPVNSMPFPMKKESSLVAQPASSSTLEAVREELGDCRRCKLHPTRTTIVFGTGNPKAK
                     LVFVGEGPGRDEDLQGKPFVGLAGQLLTKIIQAIQLSREEVYITNIIKCRPPGNRNPE
                     PDEINACEPFLIKQLEVIQPKLICALGTFAAQTLLKTEERISLLRGRFHQYQGIPLMP
                     TYHPAYLLRNPNFKRDVWEDMKKIKKEYEQL"
     gene            8379..9686
                     /locus_tag="VEL68_08820"
     CDS             8379..9686
                     /locus_tag="VEL68_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HXZ36107.1"
                     /translation="MGKKFFCAFFFLGLFLSGLLLSLLPGFLPAQEKQVNILKINDAI
                     TPAIADFIKRGIEQSVQDRAECLIIQMDTPGGLDLSMRDIIKEMMNADIPIVVYVGPS
                     GARAASAGVFITLAADIAAMAPGTNIGAAHPVAVGGGKMDRTMADKVVNDAVAYIESI
                     AEKKGRNVKWAAKAVRESVSITETEALKIKIIDLIAKDVDDLLAKIDGKTLEKSKRKI
                     KLATKGLKTNHVEMGFRERFLATLSNPNIAYILMMIGLVGLYFELSNPGAIFPGVIGG
                     ICLILAFFAFRTLPVNYAGVLLILLGVFLFIAEIKVASYGLLSVGGIISLALGSIMLF
                     ESPLPYLRASLTVILPTVLVTAAFFLLAVGLTIKAYRAKPATGMEGLAGEAGKAATRI
                     APEGKVFVHGEFWNAYSDGTIEEGEKVRVVKAENLRLKVEKLT"
     gene            9910..10722
                     /locus_tag="VEL68_08825"
     CDS             9910..10722
                     /locus_tag="VEL68_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXZ36108.1"
                     /translation="MIPSLSFLVLLILILMIAASALRILNEYERGVIFRLGRVRTVGG
                     KPQPKGPGLIFLIPIVDKLVKVSLRTVTMDVPPQDVITRDNISLKVNAVIYFRVMEPI
                     KAVIEIENYLYGTSQLSQTTLRSVCGQVELDDILYQRDKINMQIQEILDKHTEPWGVK
                     VTAVEVKAIDLPEEMRRAMAKQAEAERERRSKVIRAEAEFQAAEKLKDAAAIIGDYPM
                     ALQLRYLQTLSEVATEKNSTTIFPIPIDLIKPFLKLAERAEMSERAEKKSTS"
     gene            11166..12353
                     /locus_tag="VEL68_08830"
     CDS             11166..12353
                     /locus_tag="VEL68_08830"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36109.1"
                     /translation="MELWKRNLYSLWTVEFLAVLGMALVLPFLPFYVRELGVRELEDV
                     KRWSGFIYAAPFFLAVFTGPLWGYLGDRYGRRMMLVRAVFGFAATSLLMAFAQNVTQL
                     FFLRLIQGGVAGFIAATLAIVATTTPREHMGYAMGILQTSLTTGGVIGPLVGGLLADL
                     IGYRNIFFLTGGFGFLAGFMVILLVREEAKRGEDKKSAGFFSNYRFVFTSSTLTAIFV
                     SSMIVQLAVMAIQPVLSLFVERLWPSAETLSTMAGTVFGIVGVSSLLAAPYWGKRGDR
                     MGYKKILAITTLATGISYAPQGLVTDVYQLLILRFIQGLFVGGILPALYTLTSLNTPE
                     ERRGGIMGITRSGLLIGNVAGPISGGFLAASLGMRPVFFFTAALLISVTFAARRLIDE
                     PPH"
     gene            12469..13341
                     /locus_tag="VEL68_08835"
     CDS             12469..13341
                     /locus_tag="VEL68_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXZ36110.1"
                     /translation="MIDLHIHTNASSDGQYSPREIFEMARQKSLKAIAFADHNSLENV
                     EEGIRLAEEFGIEFIPCMELNTLYREMDLHLLAYFLDPADSVLQEWLEEIRRKKVQQA
                     DKRLEKLNELGFFFTAGDLRKFSEDRIPTGRSYLQAILSRKENLKDPRLRPYIDGNRS
                     RSPYVNFYRDYLRGGKPAFVPMEDVSTFSAIRKVRQLGAIPILAHPSDTGSDNLLALV
                     GNGLEGLEAYSPYHDPSEQEAFRAFAEHHGLLVTAGSDFHGEKIKPDIELGQVSDHPP
                     DVLEKLKERRKDRR"
     gene            13338..13982
                     /locus_tag="VEL68_08840"
     CDS             13338..13982
                     /locus_tag="VEL68_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXZ36111.1"
                     /translation="MNASNVRGVIFDLDGTLIDSYQAIYLGFHYTYSELGLPPLSHAE
                     VEKAIGLGLSYTFRELLGEERVAQALTLFRKKYEEVFRAHTHLLPDVHQVLESLQGRG
                     IQLAVATNKLGRFSRTILEHFGMDKMFAVILGDGDVSQNKPDPEMLYLAMEKMGVGKE
                     ETIFVGDSVIDIQTGRNAGIRVFAVPTGNAGREDLVKAQPTLILSRLLDLLSLV"
     gene            14189..15334
                     /locus_tag="VEL68_08845"
     CDS             14189..15334
                     /locus_tag="VEL68_08845"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36112.1"
                     /translation="MPLDEIVQSLVKVSPREKSFLSVYLDLRPEGKGKKLHAVFLKNR
                     LPELGRVLPARSREQTLLARDIRRVQRYLEEKLDPSWRGIAMFACAAEDLFLPIPLPL
                     PPENELALSPQPHLFSLVRQADLYRPYAVLAADSRQARLFWIQEGRLEKQRSLVWEEE
                     HTTRFGRMGWSLQKFQRHLQGHTRQRMKEAVETLVKWVGRGKMDYLFTVTEEGMEGEI
                     KKHLPSALRKKQSSLTSVDSRDPDHKILSGAFKAIQTLLRERAEALVRQLLDEAQPMG
                     RGTAGPEPTLSALQNHQVERLILDGQFQAEGWQCQVCGYLGTGGVPRSCPLCQGKTSP
                     APLREAIVLLAQSQGINLSFTDRFSPLVKAGGIGAMLKFNISRRRKR"
     gene            15387..15893
                     /locus_tag="VEL68_08850"
     CDS             15387..15893
                     /locus_tag="VEL68_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HXZ36113.1"
                     /translation="MALWEQVKGKTQAVQIKLRMLFLENQIDQVLLRLGSRTYDLNKS
                     GGPFGEDGEVGTLLQEISTRKKELGQLKEDFQRSWSEESKELKTRLEKGDGALEQVEI
                     SFLSPAAGKKIKEVDLPKEVLLGPILRGEELIIPDGETELQATDRVTLLGKKKDVEST
                     ARLLKGMP"
     gene            16089..16760
                     /locus_tag="VEL68_08855"
     CDS             16089..16760
                     /locus_tag="VEL68_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36114.1"
                     /translation="MAKHCRNGVYGAIFALLLGLISLAGCAASKTYLLNLSYNPLGTP
                     QFLPISAKPATLAIYQFEDARPDRLYLGRRVYRDGMVDFFKPDAGTVEQVMNQSVVKA
                     MEKAGFKITRVNRLLNQDKEDFKSIPGDVALGGRIEALWVEAKSGYVTTDTDAKLRIK
                     VVWGLPKEQVWMTKTIEGSAQETERPFYEPKYAEAKINEVFKDGMDKFLKDENQLREK
                     ILKEK"
     gene            complement(16857..17069)
                     /locus_tag="VEL68_08860"
     CDS             complement(16857..17069)
                     /locus_tag="VEL68_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXZ36115.1"
                     /translation="MPIYEYQCCKCAHQFEMIHGVSDKGSKITCPKCGAANPQRMMSA
                     FSCGGPKAGESGSASSCAPRPGSRFS"
     gene            complement(17346..17678)
                     /gene="trxA"
                     /locus_tag="VEL68_08865"
     CDS             complement(17346..17678)
                     /gene="trxA"
                     /locus_tag="VEL68_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXZ36116.1"
                     /translation="MGEGTNLLAGTDTNFQKEVLEAEQPTFVDFWASWCGPCRMIAPI
                     FEELSNQYAGKVKFVKVNVDENPKTPANYGVRGIPTLIMFKGGKVVDQVVGAVPRNQL
                     ENLVKKVL"
     gene            complement(17758..18702)
                     /locus_tag="VEL68_08870"
     CDS             complement(17758..18702)
                     /locus_tag="VEL68_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HXZ36117.1"
                     /translation="MSHKLERVLFVTSFIPVASFKSIARLGDATWGQAKIAVVIGFAL
                     ALIQFVSARKVLKYNTYLEKAFLGFLLAGTVWVYLLPENPAHIFVDHSVALLYFTLFL
                     MSLIPQLVGYEPFTYTVAKQWYPESVWGTPDFRLINYRITYVWSGVFLACFLSSFLGK
                     GKPLYSILIPFALCIGIGVVFSKKYPDVYLKRKYRVTPEAAAAIPDSVGKLIEGMPDA
                     FDPAAAGDLKAEVQFDISGEEGGKWVISIAGGRCQVRRGEALSPTVTIESPGEVWVKI
                     ARGEIDRPKALMEGLYRVKGDMKVLSRMPQLFGARRKK"
     gene            18790..18969
                     /locus_tag="VEL68_08875"
     CDS             18790..18969
                     /locus_tag="VEL68_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXZ36118.1"
                     /translation="MEGRRQREDLAKEIEALKEELMDREKALPAHTIRPHQLLAIEEL
                     EEKIRRLEEILRSLS"
     gene            19290..20660
                     /gene="radA"
                     /locus_tag="VEL68_08880"
     CDS             19290..20660
                     /gene="radA"
                     /locus_tag="VEL68_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXZ36119.1"
                     /translation="MVKVKTVFGCQNCGYQSPKWLGKCPDCGSWNSFVEEVVSPAADR
                     PGTSLAISVQDIPPQSILSIDIKEEGRQKTEISEFDRVLGGGLVVGSIVLIGGDPGIG
                     KSTLLLQALDRLSRKKIQVLYVSGEESLRQTKMRADRIGISSPDLYVLAENSLEKIIE
                     YIRKLKPRAAVIDSIQTVFTSQLQSAPGSIGQVRESAGKLMMLSKSTGLSTFLIGHVT
                     KEGAIAGPRVLEHMVDTVLYFEGDRGHPYRILRAVKNRFGSTNEIGVFEMKDGGLEEV
                     ANPSELFLAERPIDVPGSVVISSLEGSRPILVELQALVSPTSYGMPRRTAIGVDPNRV
                     SLLVAVLEKKVGMNLAQQDIFVNVAGGVRIDEPAADLGVICAVASSFLDKPIPSGTMV
                     IGEVGLAGEVRGIHQAETRIKEAFKLGFKRCLLPASNRSRIALELEGMEMVGVQSVEE
                     ALEILF"
     gene            20878..21171
                     /locus_tag="VEL68_08885"
     CDS             20878..21171
                     /locus_tag="VEL68_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36120.1"
                     /translation="MGKKGRRGIFFLGALLLIGLLVGGCGEDLKAANEKLKKEAAELT
                     AENERLKMETARLRAELSAVHGQVAELNLKVSTLQEENNTLQKEMETLKKRRR"
     gene            21190..21777
                     /locus_tag="VEL68_08890"
     CDS             21190..21777
                     /locus_tag="VEL68_08890"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HXZ36121.1"
                     /translation="MKTTGIILAGGKNLRMGQNKAFLEVQGERIIDRIKRIFVDLFDE
                     VVLVTNSPRDYLDLNLRIVADLYRERGSLAGIFTGLFHASFSHAFVAACDMPFLNPAL
                     IAHLVGLSPGYDIVIPRTEDGLQPLHAVYSRKCLPFMEDLLREGNLKILDFFHRVKKR
                     EVPAEEMIPLDPHLSSFLNLNSPEDLSRIQEYSRF"
BASE COUNT         5170 a         6160 c         5933 g         4902 t
ORIGIN      
        1 ttccctgcgg ggtcggtcaa acttctggcc tcggaacgtt ccacgggaaa ggagctgggg
       61 tttaatggga agccggtgaa agtggaaaag ttgagcaagg attccttcaa gggaattcaa
      121 atcgcccttt tttccgccgg agcgtcccgc agcctggagt ttgccccggc agctgccgcc
      181 tctggagcgg tggtggtgga caacagcagc gccttccgca tggacccgga gattcccctg
      241 gtggtgcccg aggtcaaccc ccacgccgtc gcccagtata agaaacgggg catcattgcc
      301 aatcccaact gctctaccat ccagatggtg gtggctttaa agccgatcca tgacgcggcc
      361 cggatccaga ggatcgtggt ctccacctac caggcggttt ccggcaccgg gctgaaggcc
      421 atcgatgaac tgttggttca gacccgggct cttctcgcct cccaggaggt ccagaagaag
      481 gtgtatcccc accagatcgc cttcaactgc ctcccccata tcgatgtttt tctggagaat
      541 ggctacacca aggaagagat gaagatggtc aacgaaaccc ggaagatcat ggaagacccc
      601 accatccgcg tcaccgccac caccgtccgg gttcccgttg tccacagcca ctccgagtcc
      661 agcaacatcg agaccgaaaa gaaaatcacc ccccaacagg ttcgtgaaat cctctccaaa
      721 gcccccggcg tcaaggtggt ggacaacccg tccctcaacg aatatccgct ggctatccat
      781 gctgccggac gggacgagac ctttgtgggc agaatccgcg cggacgagtc gatccccaac
      841 gggatcaata tgtgggtggt ctccgacaac gtccgcaagg gagcggccct gaatgccgtg
      901 cagatcgccg agattctgat cgaaaaatat atctgacccc taccccccaa tttcccttcg
      961 caaagtgact atatggtaac gggcggagac gccgtcattc ctgcgaaagc aggaatccag
     1021 gtctttgaat ttgttgttca ctccaacagc ctggatgccc gcctccgcgg gcatgacgaa
     1081 ttacgacaca gtctcaaagg gaggcagggc ggatcgaagc gatctctttt ttgtgatatg
     1141 cttgttcaat gaaattcctc caagacatca ccctgggaca gtaccttcat gggaattcct
     1201 tcctccaccg cctggacccc cggactaaat tcctgtccct gatcctattg atggttgtca
     1261 ccttcacggt gcaggatttc ctgcccatgg tcctgctttg gtttcttttc ttcttgggat
     1321 tggccgtttc tggcttgccc tgggcttacg tcttccgggg agttcgatcc ttcctctggc
     1381 tcttcctgtt caccgcttgc cttcatttct tttttacccc cggcccttcg cttccctttt
     1441 tccccattgg tttcctggac ctgacctgga ccggattggc caagggggtg tttgtggccg
     1501 cccagctttt tctggccatt cttctctcct cccttatgac ccttaccacc tctcctctgc
     1561 agctcgctca cgggctggag aagctcctgg ctcccctgaa acgtttccgg gtcccggtgg
     1621 aagatttctc catgatgacc atgctggcga ttaagttcat ccccatcctc ctgggagaag
     1681 caaatcggat catcaaggcc cagagcgccc ggggagtaga cttcgaatcc ggaagctttt
     1741 tccggcgggt aaaaaatttg ctatccatct taacccctct ttttcacagc gtcttcaaac
     1801 gggccgacga tctggccatc gcccttttgg ctcgaggata tctcagtggg gtcaaacgaa
     1861 cccaccttca cgaaccccaa atgacgggga aggactactg ggtgctggcc tgcgtggtag
     1921 gatttatggt tttcgagcta gagctcagcg gataggtcgg atagtcaatg agcaatgacc
     1981 aatgggcaat gggcattgtt cattggtaat tgattattga ccattgagtc tttttaggag
     2041 acttcatgag aaagaagaaa ttcaatggcc tgatccaggt ttacaccgga aacggcaagg
     2101 ggaaaaccac tgccgctctt gggctggctt tgcgggcggc gggccaccgc ttcagggtac
     2161 tcatggtcca gttcctcaag ggaggaatcg cttacgggga gttgaagtcg gcgaggaagc
     2221 tctcccccta tcttaccatc gtacccatgg ggcgggaatg cttcgtggac aagaaaaatc
     2281 cccattccgt cgaccgccgg tgggcccgaa aaggttggca cctctcgaaa gaggcggttc
     2341 agagtcagaa atatcaggtg gtcatcctgg atgaagtgaa tgtggcggta gattacggcc
     2401 tagttccttt ggatgaactc ctgcatttga tgaaaaataa acccgaaaac gtcgagttag
     2461 tcctgaccgg ccgttgggcc acacccgaag tcctccggca agccgatctg gtcacggaga
     2521 tgaaagagat caagcactat taccggaaag gaatcgagag ccggatcggg atagaacgat
     2581 aagaggcaga gcgcatggag gatagcgcat agccaattgg aaccggaccc tttgcctttt
     2641 gctctatggt ctctgccttt gtgccttgag cctttctctt ctccatgtta gaatcaatct
     2701 cgctccagag cagcagaagg ggaataagga tcttttgcgg gtcatcagcg gcaagtgcaa
     2761 gggaaagaag ctttttgcat tgaagggcct gagcctccgg cccacctccg accgggtgaa
     2821 ggaggctatc tttgacatcc tgcagaaatt tccccccaag aaaaatgtgt tggacctttt
     2881 tgccgggacc ggagctttgg ggattgaagc cttgagccgg ggtgctcaaa gggctgtctt
     2941 cgtagaggga agcgcccggt ccggtgcggt cctgcggaga aatattgagg cctgccggtt
     3001 aagcggccaa gccgaagtcg tttccaagga agttcagaca ggactgaaag ctctggaaga
     3061 acgggaggat tcattcgacc tgatttttct ggatccgccc tacgggaagg gattggcttg
     3121 tcgggctctt gaaagcctct cccgaagcca cattctttcg gtcaacgccc tgatcgttgc
     3181 cgaacattct ccggatgaag acttgagttc gatctcttca ttagagcgaa ttgaccggcg
     3241 aaagtacgga ggaacggaag tttccctctt tcgacggaag gaaaaagaat aaaatgggac
     3301 acagatgaac gtcgacctaa agaacacgaa aacccctttt aggtttttta gaattgaagg
     3361 ggccttgttc ctttttttgc gttcatatgg gtcccataag aaacgaagag gacttgtcta
     3421 tgagtgaaag aatcgcggtc tatcctggtt cctttgatcc tatcaccaac ggccacgtaa
     3481 atctgatcga gcgagccctg caagtgttcg acaaactcat tgtggcggtg gcccacaacc
     3541 ccaacaagac ggcccttttc tcggtggaag agcgaattga catgatccgc atcgctctga
     3601 agaacgaccc ccgggtcagc accgcctcct tccaggggct actggtggaa ttcgccgtcc
     3661 agcagggggc caacgtcatc gtccggggcc tgcgggccct ttcggatttc gagtacgaat
     3721 ttcagatgac ccttatgaac cgcaagctca accgcaaggt ggacaccatg ttcctgatga
     3781 ccggatataa atggttctac accagttcca agatcatcaa ggaagcggct tccctgggag
     3841 ggtcggtgcg gggcctggtc ccggagatcg ttcaccaaag gctgaaggag aaatttccag
     3901 cctaccggaa tctaccataa agaagcggtc agccatcagc ggtcagcttt cagccaagac
     3961 tttccaattg tgggctgaag cgtgacggct gatcgctcat cgcgatgaaa ggagcatccc
     4021 atgaaactcg ccgaacgcgt gaatcgcatc aaaccttccc ctacctttgc cgcggcggcc
     4081 aaagctgcgg ccatgcgggc caaaggaatc gaagtcatca gctttgctca gggtgagccg
     4141 gattttgaca ccccagacaa tatcaaggag gcggcttaca aggccctccg ggagggaatt
     4201 accaaataca ccccggcctc tgggtttccc gacctcaagg acgccatcct ccagaaattc
     4261 aagagggaca acggcctcac ctatgaccgt tcccaggtct tcgcctccgc cggggccaag
     4321 ctcatcatct acaacctggc gcaggcgctc ctccaggccg gggacgaagt aatcgttccg
     4381 gccccctact gggtttccta tacagacatc gtcctgctga tggacgcaac cccggtcgtg
     4441 gtccagacct cggaggaatc gggctttaag atgagcgtat cccagctcaa agccgcccta
     4501 accccccgca cgaaagcgat cattctcaac agcccctgca accccaccgg cgcgacttat
     4561 actgcagcgg aattgaaggg cttggttgaa cttcttcttc ccaggaaaat cctaatcgtc
     4621 gccgatgaca tctacgaaaa attcgtttat gacgggacgg tctttaccag catcgcctct
     4681 ctaagcaagg aagtgcagga tcaaaccatt gtgatcaacg gcgtctccaa gacctattct
     4741 atgaccggct ggcgaatcgg gtatgccgcg ggcccggctg atcttatggc cgccatggac
     4801 aaggtccaga cccagaatgt ttccaatccg gtctccttct gtcagaaagc ggccgtcgag
     4861 gccctgaccg gaccccaaga ttcggtgcag aagatggtca ccgaattcga ccggcgacgc
     4921 aaatatatcg tccagcgcct gaacgccatg cccggagtca aatgcgccat gcccaaggga
     4981 gccttctatg ttttccccaa catcaccggc ctcttcggaa agaagtgggg agacaaagtg
     5041 atctccaact ccggggatgt gaccgatttg attctggagg aagccaaggt ggcggtcgtg
     5101 gccggaagcg ggttcggcaa cgaccgtcat atccgcttct cctacgccac ctccatggcc
     5161 aacatcgaga agggaatgga ccggattcag gaagccctga gaaaattgaa ataagccctc
     5221 agcggtcagc tttcagtcat cagcattttc tgctgctaaa agctgaccgc ttcttttcaa
     5281 aataatcccg caaaatctgt tcatgatcga aggccagcgg gagagggaga ttattctccg
     5341 aaaatatccc gatcttcccg gcatcatcac cggctctcgc cattcctttg gctttggcaa
     5401 tgaaaaccgt tgaaatattg tgctgccgcg ggtccctctg cgggtctgag taggtatgga
     5461 actgttccag cgattcaatt ctcagccctg tttcctcccg agcctcccgg gccgcggcgt
     5521 tctccagggt ttccccgtag tctacaaacc cacccggtag ggcccatgcc cggggttcat
     5581 ttttccggta gatcaaaacg atacccctct tccccccatc tggctggacc tctatgatga
     5641 tatccacggt gggaaaagga tttgagtaaa cgaagacctc agcgccgcac tgggggcaga
     5701 ttattttttt ggttcccata taaacccctt tctggaaaat tccgggttcc caaataatcc
     5761 ttcaaatgtt ctcaggtcct ggctccgatt ctaccgaatt ttcccttgaa tggaaaattc
     5821 atccaattcc tttccctggg aaaggcccat aagttccttg acatttaccc gtttccttga
     5881 tataaattac tatcctttag gaggttaagc caatgaaaag atttgaattt ttgatctcac
     5941 caattgttct ctttctcttt ctggggctgg tcgcgggttg tgctatttct aaaaaagaag
     6001 aggggaaacc cccttcggat acgagtctca aagcttcttt ctctgatttc gaaggggttg
     6061 taattccctc tgaactttcc ctgaacaaaa aaaaatctta cgtctacagc gcagcccgta
     6121 ccaaagtggg cctcctcact tttgaagggg gagtcgaacc cgattctctg gccaccttct
     6181 tccagaccaa tctcccccgg ggcggatgga agccgatgac tgtcctgaaa gaccgggagc
     6241 attcgctgat ctttctgaaa gacgaccggg tatgcctgat aaccatctcc gagacatggt
     6301 tcaccaccat ctgcgaggtc cgagttggcc tggtggaaaa aaccccagag ccaagtaagg
     6361 aaacacccac tcgctgattg atcgtcccta gaatcataaa gatgctgaaa ggcaaaaaaa
     6421 tcgtcctcgg agtcacagga ggaattgctg cctataaggc ggcagaactc gtccgggaac
     6481 tggttcgttc gggggcggaa gtttttgtgg tcatgacccg tagcgcccag gaattcatca
     6541 ctcccctcac cttgcagacc ctttcgggga ataaagtagc cactgagctt ttcagtctcc
     6601 tcgaggaatc ggagatcggc catatctccc tcgctgaccg ggccgatatc ctggtcatcg
     6661 ctccggccac cgctaacatg atcggaaaga tcgccggagg cattgcggat gacatgctaa
     6721 gcaccgtcgt catggccacc caggcgcccg ttctattggc cccggccatg aatgtacata
     6781 tgtgggaaaa ttccatcacc caggaaaata tccagaaact ccgggcccgc ggctatcact
     6841 tcatcgatcc agaagccgga gaactggcct gcggctatga ggggaagggg cggctggctg
     6901 agatcccggc gatcgtcgaa gaggtccaga cccttttgtc cccgaaggac tactccgggg
     6961 aaactattct ggtcactgct gggcccacgg aagagcccat tgacccggtc cgttttctca
     7021 gtaatcgctc ttcgggaaag atgggctttg ccgtctcccg ggctgcccgt cggaggggag
     7081 cccaggtcac cctggtgagc ggccccaccg cattgactcc tcctccccat ctccgcttca
     7141 tccctgttcg cacggcggcc caaatgaggg aagcggtgct ggaaaatctg caggcggcct
     7201 ccatcgtggt catggccgcg gcggtctccg acttccgtcc ccagggaacc agggaggaaa
     7261 agattaaaaa atcaaaggcc aacttgaatc tcctcctgga gctcaacccc gatatcctgt
     7321 acgaagcagg tcagaagaag ggaacccggc tgctcatcgg ctttgccgcc gaaacccatg
     7381 acctgctgca gaatgcccgg cagaagctgg ccgaaaagaa cttggatttg atcgtggcca
     7441 acgatgtgag tctccccggc gcggggtttg cggcagatac caacatcgtg aagctgatcg
     7501 atcgccgggg aaacatcgaa gagcttccgc tgatgggcaa ggaagaagtg gccgacctga
     7561 ttctggaccg gctggtgaag ctgagaaccc aatgaatgcc aagccgtcct ctctaggatt
     7621 ggaccatccc cgtttcctcg tggaactgag ggagctgctg gtgggtcttc gccaccacct
     7681 ggaagaagaa aaatctttgg gcttggaggg ttggccccgg agttcaccgg tgaattccat
     7741 gccttttcct atgaaaaaag aatcttccct cgtagcccaa ccagcctcct cctccaccct
     7801 ggaggcagtc cgggaagaac tgggagattg ccgaaggtgt aaacttcatc ccactcgaac
     7861 gactatcgtc tttggaacag gaaacccgaa ggccaagctg gtcttcgtgg gcgaaggacc
     7921 cgggcgagac gaagacctcc aggggaaacc tttcgtcggc ctggccgggc agctcctcac
     7981 caagatcatc caggccattc agctcagccg ggaggaggtc tacatcacca acattatcaa
     8041 atgccggcct ccgggaaatc gaaatccaga gcccgacgaa attaatgcct gtgaaccttt
     8101 tctcatcaaa cagctggagg tcatccagcc caaattgatc tgcgccttgg gcacttttgc
     8161 cgcccagacc cttctgaaga ccgaggaaag gatctccctc ctgcggggaa ggtttcacca
     8221 ataccagggg atccccctta tgcccaccta ccaccccgct tacctcctgc gcaaccccaa
     8281 tttcaaaagg gacgtgtggg aggatatgaa gaagattaag aaagagtacg aacagctgtg
     8341 aattcctccg ctccggcgga ttcgagggag tttcgataat gggcaagaaa ttcttctgcg
     8401 ctttcttttt cctgggcctt tttctttccg gcctgcttct ttctctgctc cccgggtttc
     8461 tccccgccca ggaaaaacag gtcaacattt taaagatcaa cgacgccatc actccggcga
     8521 tcgccgattt tatcaagagg ggcattgaac agtcggtcca agaccgggcg gagtgcctga
     8581 tcatccagat ggatactccc ggcggactcg acctctccat gcgcgacatc attaaagaaa
     8641 tgatgaatgc cgatattccc attgtcgtct acgtaggtcc cagcggagcg agggcggctt
     8701 cggctggggt tttcatcacc ctggccgccg acattgccgc catggccccg gggacgaata
     8761 ttggagccgc ccatccggtg gccgtggggg ggggcaagat ggaccggacc atggcggaca
     8821 aagtggtgaa cgacgccgtc gcctatattg aatccatcgc cgaaaagaag ggaaggaacg
     8881 tgaaatgggc ggccaaggcc gtgagggaga gtgtctccat caccgaaacc gaagccctga
     8941 agatcaagat catcgatctc atcgccaagg atgtagacga cctgctggcg aaaatcgacg
     9001 gcaaaaccct ggaaaagtca aaacgcaaga tcaagctggc caccaagggt ttgaagacca
     9061 accacgtcga gatgggcttc cgggaacgct tcctggcgac cctgagcaac ccgaacatcg
     9121 cttacattct catgatgatc ggcctggtgg ggctctattt cgagctctcc aacccggggg
     9181 ccatcttccc aggggtcatc ggggggatct gcctgatcct ggcctttttc gcgttccgga
     9241 ctcttcctgt taactatgcc ggagtgctgc tcattctcct aggggtcttt ctctttattg
     9301 ccgagataaa agtagccagt tacggcctcc tttccgtagg gggcatcatt tccctggctt
     9361 tggggtcgat catgctcttt gaatcccccc tgccctatct gcgggcctcc ctgacggtca
     9421 ttctcccgac ggtcctggtt acggcggcct ttttccttct tgccgtgggc ctgacgatca
     9481 aagcctaccg ggcaaagccg gccacgggga tggagggtct ggccggggag gcgggaaagg
     9541 ccgccacgcg gattgccccg gagggcaagg tctttgttca cggggaattc tggaatgcct
     9601 acagcgatgg gacgatcgaa gagggagaga aggtccgggt ggtcaaggca gagaatctca
     9661 ggctgaaggt ggaaaagcta acctgatcag catagcaaaa ttccgcgggg gacgaaatta
     9721 aaaaatcgga gaatctatag tcaacggctc aaaaaaagga gtcatcgcga ggagcgaagc
     9781 gacgcggcaa tctcatgaga tcgcttcact tagttcgcaa tgacagcgcc ttgaatgtca
     9841 acttatttag cccgtcacta tacttgcaac gactgagttt tcaacaaatt ctaaggatag
     9901 gaggttacaa tgattccatc tctttcgttt ttagtcttgc tgattctgat cctcatgatc
     9961 gcggccagcg cgctgcgcat tctcaacgaa tacgaacggg gagtcatctt ccggttgggc
    10021 cgggtacgca cggtcggggg gaaaccccag cccaaggggc ccgggctcat ctttctcatc
    10081 cccatcgttg ataagctggt gaaagtcagc ttgcggacgg taaccatgga cgtccccccc
    10141 caggacgtca tcacccggga caacatctcc ctcaaggtga acgcggtcat ctatttccgg
    10201 gtcatggagc ccatcaaagc ggtcatcgag attgaaaatt acctctatgg tacctcccaa
    10261 ctgtcccaga cgacccttcg ttcggtttgc ggccaggtgg agctcgacga tatcctgtac
    10321 cagcgggata agatcaacat gcagatccag gaaatccttg ataagcacac cgaaccctgg
    10381 ggagtgaagg tcaccgccgt ggaagtaaag gccatcgacc tgcccgagga gatgcgccgg
    10441 gccatggcca aacaggcgga agccgagcgt gagcgtcgat ccaaggtcat ccgggccgag
    10501 gctgaattcc aggcggctga aaaactaaaa gacgccgcag cgattatcgg cgactatccc
    10561 atggctctgc agctccgcta tctccagacc ctttccgaag tagccacgga gaagaactcc
    10621 accaccatct tccccatccc catcgacctg atcaagcctt tcttaaagct ggcggaacgg
    10681 gcggaaatgt cagagcgggc ggaaaagaaa tcaacgagct gagcatagcg tttgcctggg
    10741 gtaaaatagg atgggatcct ggagggtccc atcctttttt tcgatccctc ttttgatttt
    10801 ttaaccgcag aggccgcaga gaacgtcgag aaaaggcaaa aaataagctc caaagaaccg
    10861 aaagcaataa ccgcctttga attcattcct tccgttttca tcggcagttt tgtttttttg
    10921 taacagttta gtggtttgac acggctttcc cagagatccc ccccgccctc ccttttccaa
    10981 agggagaaga gattatttac gggaatcgat gaatcattaa actttttccc cctttgaaaa
    11041 agggggacta ggggggattt gacagttttt caaaaggcta aatggttacg tttttcctgt
    11101 ttgatttatt ttgattgaaa gcatctctct gcgttctctg cgctctctgt ggtggaagtt
    11161 tttttgtgga actctggaaa cgaaatttat acagcctctg gacggtggaa tttctggccg
    11221 ttctgggcat ggccctggtc cttccctttc ttcccttcta tgtccgggaa ttgggagtca
    11281 gggaactgga ggacgtaaag cggtggagcg gcttcatcta cgccgccccc tttttcctgg
    11341 ccgtctttac cgggcccctc tggggttatc tgggggaccg ctacggccgg cggatgatgc
    11401 tcgtccgggc cgtcttcggc tttgccgcaa cttctctgct catggccttt gcccagaacg
    11461 taacccagct ttttttcctt cgcctaatcc agggaggggt cgccggattc atcgccgcca
    11521 ctctggccat tgtggcgacc accactcccc gcgaacatat ggggtatgcc atgggaatcc
    11581 tccagacctc cctgaccacc gggggggtca tcggtccctt ggtgggcgga ttattggcgg
    11641 acttgatcgg ataccgcaat atcttctttc tcaccggcgg gttcggcttc ctggccggat
    11701 tcatggtcat tctcctggtt cgggaggagg cgaaacgcgg ggaggacaag aaatccgcag
    11761 ggttcttttc caactaccgc ttcgttttta cttcttccac tctcaccgcg atctttgtct
    11821 ccagcatgat tgttcaactg gccgtcatgg ccatccagcc ggtcctttct ctgttcgtgg
    11881 aaaggctctg gccctccgcc gaaactcttt ccaccatggc cggcaccgtt tttgggatcg
    11941 tcggtgtttc cagccttctc gccgctccct actggggaaa acgaggagac cggatgggtt
    12001 ataagaaaat cctggccatc accaccctgg ccacgggaat ctcctacgct ccccagggcc
    12061 tggtgaccga tgtgtatcag ctgctcattc tccggttcat ccagggcctc tttgtgggtg
    12121 ggattctccc ggccctgtac accctgacct cgctgaatac tccggaagaa aggcgggggg
    12181 ggatcatggg aatcacccgg agcggtctcc tgatcggaaa tgtggccggg ccgatctcgg
    12241 ggggcttctt ggccgcttcc ctgggcatgc gcccggtgtt cttcttcacc gcggccctgc
    12301 tgatctcggt cactttcgcc gccagaagac tgattgatga acccccgcac tgaaaggttc
    12361 aaaaggttgg aggaccgtca cgccgggggc gtgactcaag gatcgctccg cttgaggctc
    12421 aagtgaggag ataagccgga aggatctccc gcaccctgca ccttttttat gattgacttg
    12481 catatccaca ccaatgcctc ctccgacggg cagtattctc cccgagaaat cttcgagatg
    12541 gcccggcaaa aaagccttaa ggccattgcg ttcgccgacc acaattccct ggaaaatgtg
    12601 gaagagggga ttcgcctggc cgaagaattc ggcatcgaat ttatcccctg catggaattg
    12661 aacacccttt atcgggagat ggacctccac cttctcgcct acttcctgga cccggccgac
    12721 tccgtgcttc aggagtggct ggaagagatc cgccggaaaa aggtacagca ggccgacaaa
    12781 cggctggaga agttgaatga gctgggattc ttcttcactg ccggggattt gcgaaaattc
    12841 tctgaagacc ggattcctac aggccgatct tacctgcagg ccatcctcag ccgcaaagaa
    12901 aacctgaagg acccgcgtct ccgcccatac attgacggga atcggtcccg ttctccctac
    12961 gtgaatttct accgggacta ccttcgggga gggaaaccgg cctttgtccc catggaggat
    13021 gtctccacct tctcggccat ccggaaggtc cgtcagctgg gcgccatccc cattctcgcc
    13081 catccttcag acacgggctc agacaacctc ctagccctcg tggggaacgg cctggaaggc
    13141 ctggaagcct attcgcctta ccatgaccct tcggagcagg aagccttcag ggctttcgcc
    13201 gagcatcacg gtctcctggt gaccgccggg tcagattttc acggggaaaa aatcaagccg
    13261 gatatcgagc tgggccaggt ttcagaccac ccccctgatg tcctcgaaaa attgaaggag
    13321 agaagaaagg atagacgatg aatgcaagca atgtccgggg ggtgatcttc gacctcgacg
    13381 gaaccctgat cgattcctat caggccattt acctgggttt ccactatacc tattcggaat
    13441 tgggacttcc tcccctatcc cacgccgagg tggaaaaggc catcggcctg gggttgtcct
    13501 atacctttcg ggagctgctg ggagaggaaa gggtcgctca ggccctcacc cttttccgaa
    13561 aaaaatatga ggaggttttc cgcgcccata cccacctgct ccccgacgtg catcaagtcc
    13621 tggaaagcct tcagggtcgg ggcattcaac tggccgtggc cacaaataaa ctcggccggt
    13681 tctctcggac gattctcgaa cattttggta tggataaaat gtttgccgta atcctgggag
    13741 acggagatgt ttcccagaac aaacccgatc ctgaaatgct ttacctggcc atggagaaga
    13801 tgggggtggg gaaggaagaa accatctttg tcggtgattc ggtgatcgat atccagaccg
    13861 gcaggaatgc gggaatccgg gttttcgcgg tccccacggg gaatgccggc cgagaggatt
    13921 tggtcaaggc tcagcccacc ttgatcctga gccgtctcct ggaccttctt tccttggttt
    13981 gaccgaaggc tgggcctgga aaaggcatgg ggagcatcgg aaaaaagtcc gttgcacaag
    14041 cctcccggct gggggtacaa tcgggacagg aaaagaattg cggtcagggg agaaattagt
    14101 ctccagaacc tattccactc agaaaggggg aaaagactct aagaagatgc ggggataaac
    14161 ttcattctct gaaaaggagg tcagcgggat gcctctcgat gaaatcgttc agagtctggt
    14221 gaaggtcagt cctagggaaa aaagcttcct ttccgtttac ttggatttgc gcccggaggg
    14281 aaaggggaaa aagctccatg ccgtcttcct caagaaccgt ctgcctgaac ttggccgagt
    14341 cctccccgct cgttcccgtg agcaaaccct tctcgcccgg gatatcaggc gggttcaaag
    14401 gtatttggaa gaaaaactgg acccctcctg gagggggatt gctatgtttg cctgtgccgc
    14461 cgaagatttg ttcctcccga tccctttgcc cctgcctccg gaaaatgaat tggccctgtc
    14521 gccccaaccc cacctgttct ccctggtgcg ccaggctgat ctgtaccgcc cttatgccgt
    14581 cctcgccgcc gactcccggc aggcccgact tttctggatt caggagggtc ggttggagaa
    14641 acaaaggtcc ctcgtttggg aagaggagca caccacccgg ttcggccgga tgggttggtc
    14701 ccttcaaaaa tttcagcgcc acctccaggg gcacaccaga cagcgaatga aggaggctgt
    14761 tgagactctg gtaaaatggg ttggcagggg caaaatggac tatcttttca ccgtcaccga
    14821 agaaggaatg gagggggaga tcaaaaagca tctgccctcc gccttgagaa agaagcagag
    14881 ttccctcact tcggtggact cccgggaccc ggatcataaa attctttcgg gtgccttcaa
    14941 ggccatccag accctgttgc gggaaagggc ggaagctctg gtccggcaac tgctggatga
    15001 ggcccagccc atggggaggg gcaccgcagg accggagccg acgttgagcg ccctgcagaa
    15061 tcaccaggtt gaacggctga tcctcgacgg acagtttcaa gccgaaggct ggcaatgcca
    15121 ggtctgtggg tacttgggca cgggaggagt gccaaggtcc tgtcctctgt gccaggggaa
    15181 gaccagcccg gcaccgttgc gggaagctat cgttctcctg gcccaatccc aggggatcaa
    15241 tctatctttt accgatcgtt tctctcctct ggtaaaggct gggggaattg gagccatgct
    15301 gaagttcaac atttcaagga gaaggaagcg gtgaccgaca gtcttactgc cctccggggc
    15361 ttacatcgtt ttactaggag gaaaggatgg ccttgtggga acaggtgaag gggaaaacgc
    15421 aggcggttca aatcaagctg cgcatgctct ttttggagaa tcaaatcgac caagtcctcc
    15481 tccgcctggg cagccgaacc tatgacctta ataagagcgg cgggcccttc ggcgaggatg
    15541 gagaagttgg gactcttcta caggaaatct ccaccaggaa gaaggagttg ggtcagctga
    15601 aggaagactt tcaaagaagc tggagcgagg aatccaagga gttgaagacc cgcctggaaa
    15661 aaggagacgg ggctctggag caggtggaaa tttcattcct gtccccggcg gctgggaaaa
    15721 agatcaaaga ggtggacctt cccaaagaag ttctcctggg gccgatcttg cggggcgagg
    15781 aattgatcat ccccgacgga gaaaccgaac tccaggccac ggaccgggtc actctcctgg
    15841 gaaagaagaa ggatgtggaa tccaccgccc gactcttgaa agggatgccc taaggaaggt
    15901 cggaatccgg ggagtagggc cgcaaaacaa cggttttccc tggcttgcct tggcgtgtct
    15961 gggtataatg gggccaaccc aggcctaagg ttttttcgat aaggaataga ggatgaagca
    16021 ttcgggatca tccttaaaga cggagtcgtc cagaattacc gattcataaa accataggga
    16081 ggaaacgaat ggctaaacac tgccggaacg gagtctacgg ggcgatcttc gccctgcttc
    16141 ttgggcttat ttccctcgca ggttgtgcag cgagtaaaac ttatctcctg aatttatcct
    16201 acaatccgct ggggactccc cagttcctgc ccatttccgc caagccagcg accttggcca
    16261 tctatcaatt tgaagatgcc cggcccgatc gactttacct gggcagacgg gtttaccggg
    16321 acggaatggt ggattttttc aagcccgatg ccgggaccgt ggagcaggtg atgaatcagt
    16381 cggttgtcaa agccatggaa aaagcgggat tcaaaattac ccgggtcaat cgccttttga
    16441 accaggataa agaagatttt aaaagcatcc ccggagacgt cgccctggga ggaaggattg
    16501 aagccctctg ggtggaagcc aagagcgggt atgtaactac cgataccgac gccaagctgc
    16561 gaatcaaggt agtctggggt ctgccaaagg agcaggtttg gatgaccaag accatcgagg
    16621 ggagcgccca ggaaaccgaa cgtcctttct atgagcccaa gtatgccgaa gccaaaatca
    16681 acgaggtttt caaggatgga atggataaat tcctgaaaga tgaaaatcag ttgcgggaaa
    16741 aaatcctgaa agaaaaatga ttcctcccat cgaactcccg agggcgggaa aaaaggccag
    16801 ggtgaactct ggccttttcc ttttcagatg ctgctcccgg cagcgcccta tcaacctcag
    16861 ctgaagcgac tgcccggccg gggggcacaa gatgaagccg acccgctctc gccagccttg
    16921 ggtcccccgc aggagaaggc cgacatcatc cgttggggat tcgccgcacc gcatttgggg
    16981 caagtgatct tcgaaccctt atccgaaacc ccatggatca tttcaaactg gtgagcgcac
    17041 ttgcaacact ggtattcgta aatgggcatt cttgaacctc cttttgaacc aatcatcgtg
    17101 gaattgcccg gtctatgagt attttcctgg gtctctaact tctcatctct gaactgattt
    17161 taaggcctta ccctaaggcc attaccaacc cgattaccgc ccccaaggtg gtgctgatgt
    17221 acgggttgca ggtcaaacct caggtgctgc cgattttaac cgaaatcagg ctcagaccat
    17281 aacccacgat ggctcctgcg gccaggcctg caataacttt ccaggtcatg gaggattcct
    17341 tccttctata ataccttctt aaccaaattt tccaattgat ttctgggaac agctccgact
    17401 acctggtcga ccaccttccc gcctttgaac atgatcagag tcggaatgcc cctcacccca
    17461 tagttggccg gagtcttggg attctcatcc acattcactt tgacaaattt aactttcccg
    17521 gcgtattgat tggaaagctc ttcaaagatc ggagcaatca tgcggcaggg tccgcaccaa
    17581 gaggcccaaa aatcgacgaa ggtgggctgt tccgcttcga gaacctcttt ctgaaaattg
    17641 gtatccgttc ctgccaataa attggttcct tctcccatca gcttcctcct tcccatttcc
    17701 atgaaaatgg ataatcaaat ttttagatgc ctcgatgccc taaaagtttc aaaaaaatta
    17761 tttttttctc cgcgccccga agagctgggg catccgggac aagaccttca tgtcgccctt
    17821 gacccggtaa agaccctcca tgagagcttt tggccgatcg atctcccccc gcgcgatttt
    17881 aacccaaacc tctccggggg attcaatcgt aaccgtggga gaaagggcct ctcccctgcg
    17941 aacctggcag cgccccccgg cgatggaaat aacccacttc cctccttctt ccccggaaat
    18001 atcgaactgg acctccgcct ttaggtcgcc ggccgcggcg ggatcaaagg cgtcgggcat
    18061 gccttcgatg agcttgccaa ctgaatccgg aattgccgcg gccgcttcag gggtgacccg
    18121 gtatttccgc ttcaggtaaa catcaggata ctttttggag aacaccaccc cgatcccgat
    18181 gcacagagca aagggaatga ggatggaata aagaggcttt cctttcccca ggaaactgga
    18241 aagaaaacag gccaaaaaga cccccgacca aacgtaggtg atccgataat tgatcagacg
    18301 aaaatcgggc gtcccccaga ctgactccgg gtaccactgc ttggccacgg tataggtgaa
    18361 gggttcatag cccacaagtt gcggtatcaa gctcataaga aaaagggtaa aataaagcag
    18421 ggccacggaa tgatccacga agatatgggc cggattttcc ggcagcagat agacccagac
    18481 cgttccggcc agaagaaatc caaggaaagc tttttccagg taagtgttgt actttaagac
    18541 ctttcgggca cttacaaact ggatcaacgc cagggcaaaa ccgatgacca cggcgatttt
    18601 ggcctgcccc caggtggcat cgcccaggcg ggcaatggac ttgaaactgg ccaccgggat
    18661 gaaggaagtc acaaaaagaa ccctttccag cttgtgagac atgcgatggc tcggaaaagc
    18721 ctttttgaat caatcccttt atggggtcat ttaatgataa caaaaacctg agggacaaac
    18781 tactgcaaaa tggaaggaag aagacagaga gaggacttgg ccaaggaaat agaagccttg
    18841 aaggaagaat tgatggatcg ggaaaaggcc ttaccagccc ataccattcg acctcatcaa
    18901 ttactggcca tcgaggaatt ggaggagaaa atccgccgcc tggaagaaat acttcgtagc
    18961 ttatcctagg ctgggtcgaa atccatgaat tgcattaacc atccaaaacg gaaagccttt
    19021 tccatctgcc agaaatataa tcaagggtac tgccaagaat gctgcacctg caccgaccct
    19081 aagggatact gcaggtatcg cacccagtgc atcgcctggc aagtctgcaa gaaaagcaaa
    19141 gggaatagag catagcgcca agactacatt tttacggctc cataccctct gctcttttcc
    19201 ctacgccgct ctttgcgatg gtccttttcc atttgacttc ttgtcccccc ggaatctata
    19261 atcagaaaaa aataatctca ttattccaaa tggttaaagt caaaactgtt ttcgggtgtc
    19321 agaactgcgg ataccagtct cccaaatggc tgggcaaatg cccggattgc ggttcctgga
    19381 attcctttgt ggaagaagtg gtttctcccg ccgcagaccg cccgggcact tccttggcca
    19441 tctccgttca agatatcccc ccccaatcca tcctgtccat agatatcaaa gaagaaggaa
    19501 ggcaaaaaac ggagatcagt gaattcgacc gggtcctcgg gggcggtctg gtggtggggt
    19561 ccatcgtcct catcgggggg gacccgggga tcggaaaatc caccctcctt ctgcaggccc
    19621 ttgaccggct ttcgcgaaag aaaatccagg tcctgtatgt ctccggggaa gaatccctgc
    19681 gccaaaccaa aatgcgcgcc gaccgcatcg ggatttcgtc cccagacctt tatgtcctgg
    19741 cggaaaactc cctggaaaaa atcatcgaat acatcaggaa actcaagccg agggcggcgg
    19801 tcatcgactc catccaaacg gtgtttacct cccagttaca gtccgctccc gggtcgatcg
    19861 gccaggtgcg ggaatcagcc gggaaactca tgatgctctc caagtccaca ggcctttcca
    19921 ccttcctcat cggccacgtg accaaagaag gggccatcgc cggccccagg gttctggagc
    19981 acatggtaga caccgtcctt tacttcgagg gagacagggg acacccttac cgaatcctgc
    20041 gggcggtgaa gaaccgcttc ggctccacca acgaaatcgg cgtcttcgag atgaaagacg
    20101 ggggactgga ggaggtggcc aatccttccg agctcttcct ggccgaacgc cccatcgatg
    20161 tcccgggctc ggtggtcatc tccagcctgg agggatcacg gcccattctg gtggagctcc
    20221 aggccctggt cagccctact tcctacggaa tgccgcgacg aacggccatc ggcgtggacc
    20281 cgaaccgggt ctccctcctg gtggcggtct tggagaaaaa ggtgggaatg aacctcgccc
    20341 agcaggacat tttcgtaaat gtagccgggg gagtccggat cgacgaaccg gcggccgacc
    20401 tgggggtcat atgcgcggtg gcctcttctt ttctcgacaa gcctattccc tccggaacca
    20461 tggtgattgg agaggtgggg cttgcgggcg aagtccgggg aattcaccag gccgagaccc
    20521 ggatcaagga agccttcaag ctgggtttca aacggtgcct cttgcccgcg agcaaccgaa
    20581 gccgcatagc cctggaactc gaaggcatgg aaatggtcgg cgtccaatcg gtcgaggagg
    20641 ccctggaaat tctcttttga agggtttccc ccttcactct caagaaaaaa catccatgtc
    20701 catggcttgg aggccgtgcc cgggccggag gtcagccata ggcggacctc cgggccgcaa
    20761 gcttactttt gcccaagaaa gaaaatcgga taaatcaatg tgggtgggtt tttgaaggtt
    20821 cttgactttt ttcccttccc cgaatatacc taaacaaaaa gcatggggag ttgatttatg
    20881 ggcaaaaaag gaagaagggg gattttcttt cttggcgctc tcctcctgat cggcctcctg
    20941 gtcgggggtt gcggagaaga cctcaaagcg gccaatgaaa aattaaaaaa agaggccgcg
    21001 gaactcaccg cggaaaacga acgattgaaa atggagaccg cccgcttgcg ggcggagctt
    21061 tccgcggtcc acggccaagt ggcggaattg aacctgaaag tctccaccct tcaggaggag
    21121 aataataccc tgcagaagga gatggaaacc ctgaagaaga gaagaagata atccatctgg
    21181 gcgggggaag tgaaaacaac ggggatcatc ctggcggggg gcaagaacct ccgcatgggg
    21241 caaaataaag cttttctcga agttcagggc gaaaggatca tcgaccggat caagcggatt
    21301 ttcgtcgatc tcttcgacga ggtggtgctg gtcacgaatt cgccccgtga ttacctggac
    21361 ctgaatctac ggatcgtcgc cgatctctac cgggaaaggg ggtctctcgc agggatattc
    21421 accggtcttt tccatgcctc cttttcccac gcctttgtag ccgcatgcga tatgcccttc
    21481 ctcaacccgg ccctgatcgc ccatctggta gggctatccc cgggttatga catcgtgatt
    21541 ccccggaccg aggacgggct gcagcccctc cacgcagttt attcccggaa atgtcttccc
    21601 ttcatggaag acctccttcg ggaaggaaat ttgaaaatcc tcgacttctt ccatcgggtt
    21661 aagaagaggg aagtccccgc cgaagaaatg atccctctcg accctcatct gtcctctttc
    21721 ctgaatttaa actcgcccga agacctgtcc cgaattcaag agtattcgcg tttttaatct
    21781 tctggcggtg cccctaaaat actttgaaac tcaattcaga agatacaatt cgctagggct
    21841 caacgcggtt ttgggtcccg tggcgcatgc attcacagtt aagagccccc cccatggggt
    21901 aattctattg gaatttcgat aaaggaattt cctgctcggc ccctcccccc cattttcgga
    21961 ggggtaaggg taaatttagg agtcacgaca aaaagccggt tgtccctatt ccagtctagg
    22021 cgctaataga tcggagggaa atcatgaccg gtccgaggtc ttgatcttgg taagcccctg
    22081 cagcaggagc tgcttgggct tccacccggc taccgaagta aagcgggact tctactggat
    22141 cgctaaagtt cccggcccga tcctg
//
LOCUS       DASPRR010000229         5568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1181098, whole genome shotgun sequence.
ACCESSION   DASPRR010000229 DASPRR010000000
VERSION     DASPRR010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181098"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..278)
                     /locus_tag="VEL68_08895"
     tRNA            complement(202..278)
                     /locus_tag="VEL68_08895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(242..244),aa:Arg,seq:ccg)
     gene            630..2402
                     /locus_tag="VEL68_08900"
     CDS             630..2402
                     /locus_tag="VEL68_08900"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXZ36122.1"
                     /translation="MAEELEGEKKEPEATDEIASFEGEEVEEVFLHARDLMNIFIKTI
                     KAFRLYPPENPSLVGFRDLLFRKFQFFLKKYQAFSFKVGEYDFSFNNRVLYENKELKT
                     SLAFFLYKDGLRELRFIEGLEEWEIQGFLEIVKRGDQINKMEDDLVTMIWERDFVHIS
                     YQATDEFLDEVPVIIPENIDQFRKHLTFEPLGHQVYDDYLVDEAGEEVNYYEAMAKAS
                     APPSLAMNRNVYLLTPEELEGLRKEVEFETSPIFLFNIIDILFEIMALEKRPEPYQDS
                     VNVLSKLLDALLTLGEFQKAADLLSRIYIILKTYDLQGWQAKAIQQLGDSAGDEQRIE
                     KIGKVLERREGVRLDEVMGYLKLLPPNSLQPLMKVLGELSNPKARRTVCDAICQIGKD
                     QIGQIIPFMEDRRWYLVRNVVYILGRIGKEAAIPSIQKALAHREMRVRREGVHSLGFI
                     GGPKAFNLLVKALQDSDVRVRCAAILNLGKVGRKNSLPYLAELVQGPEFHRKDPAEKK
                     ALFDAIGIAGSNEALPMLQKLLMKKAWFRRRRVEEAREGAAGALALIGTREAKSILEL
                     GQKSKNAAVCRVCQQALRRQAPSP"
     gene            2411..3898
                     /locus_tag="VEL68_08905"
     CDS             2411..3898
                     /locus_tag="VEL68_08905"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXZ36123.1"
                     /translation="MPKDTRGKSPSDERTDVNIPMGQEHVPEMMGTEFILRFYRLLKG
                     ATLYDRNNAIIDKLTQEALQTINPFIQSQEHLYLKIVRDNFFFNNIRIPVKADRYSIF
                     KIFSLEMARRMIGEVEFIEELTPSTLKDFVFLLSRLDEGNESNYLYVNRQLESRGVHG
                     VSVGKLEYMRDEDFLIDSEKQKHYSKQIYFKTVNLVREVSEGIRTQKLINIRKAKHLM
                     QSAVNSIMQDESALLGLTNIKNYDDYTFNHCVNVAIYAIALGQRIGIPKKQLSHLGMA
                     GLFHDLGKVSIPKEILNKTDKLSPQEWAMIRSHPVVGAEMVIRLKEWGELSARLIDAS
                     FEHHLKYDLTGYPKLARKRSITLFGKIIAIADFYDALVRPRIYRQYPYVSEKILGLML
                     ERAGKDFDPALVKIFINMIGIFPLGTLVLLNTNEIGIVFQTQEDPEMIDRPKVCLLQY
                     SDGEYHKGKAVDLREMEEGTRSFKRSIMRTLDPNEYNIHVAEFLL"
     gene            complement(3951..4379)
                     /locus_tag="VEL68_08910"
     CDS             complement(3951..4379)
                     /locus_tag="VEL68_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36124.1"
                     /translation="MTGFFLHQKSRSRTRNDVGITTPGCLVLLILIVIVGFLGFKFGE
                     AFWTYYQVREQVRQALTWTVAGGPKPEAEIVQRVISEVRSEEVQLTPRNVRITQTSDS
                     LTLIVSWTQEVELPFYTYPLDFEVSLTEIKRWGRGGLVVK"
     gene            complement(4376..4945)
                     /locus_tag="VEL68_08915"
     CDS             complement(4376..4945)
                     /locus_tag="VEL68_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXZ36125.1"
                     /translation="MNTLDIIFLVLIGASGLYSLIRGLIREVFSFLAIILGFLGASYG
                     YSRVAAWVKGWVQQETVAQIVSFAVLFLLVALGVSFLGSLLSKAIHKGGLGWADRMGG
                     AAFGVLKAILLIAIILLVLTAFLPPKSKLLLESKISPAALSIARGLSFLVPERFQSLY
                     AQKERELKKYWSFREFPMEKSENKGMKKI"
     gene            5256..>5568
                     /locus_tag="VEL68_08920"
     CDS             5256..>5568
                     /locus_tag="VEL68_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ36126.1"
                     /translation="MNRKRDLGFLFNPRSIALVGVPRGFEPGRVFLQGLLDQGYSGKV
                     FPINPKADSIDGCRAYPSLKEVPGEIDLAILLLPAALVLPALEECGEKGVKIAVIYSS
                     GF"
BASE COUNT         1377 a         1424 c         1491 g         1276 t
ORIGIN      
        1 tccttccagt ggatggagga tacctgacca tatagaaaaa ttcgaaatcc gaaacaaaac
       61 cgggggtcca aaattcaaat aaccgaaacg aacgaatcca tacgatggcg caccagaaaa
      121 gatcaagttt tggtcatcgg aatttgaagt ttgtttcgaa tttcgacatt cggatttcgg
      181 atttgttttt tagggggggg gtggtgggcg atgcggggat cgaacccacg gcctttggtt
      241 ccggagacca acgctctatc catctgagct aatcgccccg ttatgatttt taggttatca
      301 ttgaaaccca tgacaggtca acggttttcc tgggcttttc catcttccct ttttcgattc
      361 tctttcctgt ctaagagcga gcttgaggcc gggaagtctc gcctttggcg ggacttcccg
      421 gcagggcatc gcctgaattt cttcccatgt tatgactact tgacgaaaag tgaaggtggg
      481 caggcttttc atggaggcct tcttccggca aactaagaaa gaagggagaa attgtcaggg
      541 aacgagggta gggtggaaaa atattgacaa atcctttata aaacgacaag atagatataa
      601 tttaaatttg ggaagtggga aaaagattca tggcggaaga attggaaggg gaaaaaaagg
      661 aacccgaggc caccgatgag atcgcttcct tcgaagggga agaggtcgaa gaagtctttc
      721 ttcacgcgcg agatttaatg aacatcttca ttaaaaccat caaggctttc cgcctgtatc
      781 cccccgaaaa tccctccctg gtcggctttc gggaccttct cttccgtaaa ttccaattct
      841 tcttgaagaa ataccaagcc ttttccttca aggttgggga gtacgacttt tcattcaaca
      901 accgggttct ctacgaaaat aaggaattga agaccagcct ggcctttttt ctctacaagg
      961 atggattgcg cgaactccgt ttcatagaag gcctggagga atgggagatc caagggtttc
     1021 tggaaatcgt caaacgaggg gaccagatta acaagatgga agacgatctg gttaccatga
     1081 tttgggagcg ggattttgtc catatcagct accaagccac ggacgaattc ttggatgaag
     1141 tcccggtgat cattcccgag aacatcgatc aatttcggaa gcacttaacc tttgagcctc
     1201 tagggcacca ggtctatgac gactatctgg tggatgaggc tggggaagag gtcaattact
     1261 acgaggccat ggccaaggcc tccgctcccc cttcgctggc catgaaccgc aatgtctact
     1321 tgctcacccc cgaggagttg gaagggctac gaaaggaagt ggaattcgag acttccccca
     1381 tcttcctttt caacatcatc gacatcctct ttgagattat ggctctggaa aaaagacccg
     1441 agccctacca ggattcagta aacgttctca gcaaactgct ggatgccctc ctgaccctgg
     1501 gagaattcca gaaggctgcg gacctcctga gccggatcta tatcatcctc aaaacctatg
     1561 acctccaggg atggcaggcc aaggccatcc aacaattggg ggatagtgcc ggcgacgagc
     1621 agagaatcga gaagatcggc aaagttttgg agaggcggga aggggtccgc ctggatgaag
     1681 tgatgggcta cctcaaacta ttacccccca actccctgca gcctctcatg aaggttttag
     1741 gggagctcag caatcctaag gcccgcagga cggtctgcga tgcgatctgt caaatcggga
     1801 aagatcaaat tggccagatt attcctttca tggaggaccg gcgctggtac ctggtgcgca
     1861 atgtggttta tattctaggc cgaattggga aggaggcggc catcccctcc atccagaagg
     1921 cgcttgccca cagggagatg cgggtaaggc gagaaggggt tcattcttta ggctttatcg
     1981 gaggcccgaa ggcattcaac ctcctggtga aagcgttgca ggactccgat gtgcgggtcc
     2041 gatgcgccgc catcctaaac ctgggaaagg tggggcgcaa gaacagcctc ccctatcttg
     2101 ccgaactggt tcagggcccg gagtttcacc ggaaggaccc ggcggaaaaa aaggccctct
     2161 tcgatgccat cgggattgcc ggttccaatg aagccctgcc gatgctccag aagctcctca
     2221 tgaagaaagc ctggttccga aggagaaggg tggaagaggc ccgtgaaggg gcggctggcg
     2281 ctctggccct gatcgggacc cgagaggcga aatcgattct ggaattgggg cagaaatcaa
     2341 agaatgcggc ggtctgcaga gtctgccagc aggcgctgcg aaggcaagcc ccctctccgt
     2401 agaggttctg atgcccaaag atactcgggg aaagagccct tcggatgaac ggacggatgt
     2461 caacatcccc atggggcagg aacatgtccc ggagatgatg gggaccgagt tcatcctccg
     2521 tttctaccgg ctactgaagg gggccaccct gtatgaccgg aacaatgcca tcatcgataa
     2581 gctcacccaa gaagcgctgc agacgatcaa cccctttatc cagtctcagg aacacctcta
     2641 tctgaagatc gtccgggaca attttttctt caacaacatc cgcatcccgg tgaaagccga
     2701 ccggtactcc atctttaaaa ttttttccct agagatggcc cggcggatga tcggagaggt
     2761 ggagttcatt gaagagctga ctccctccac ccttaaagat ttcgtctttc tcctgtcccg
     2821 tctggatgaa ggcaatgaaa gcaactacct ctacgtgaat cgtcagctgg agtcccgggg
     2881 ggtccacggg gtcagcgtgg gaaaattgga gtatatgcgg gatgaggatt tcctcatcga
     2941 ctcggagaag cagaagcatt actcgaagca aatttacttc aaaaccgtga acctggtcag
     3001 ggaagtttcg gagggaatcc ggacgcagaa actcatcaac atccgcaagg ccaagcatct
     3061 gatgcagagc gcggtcaatt ccatcatgca ggatgagtcg gccctcctgg gactgaccaa
     3121 catcaagaac tacgacgact acacctttaa ccattgcgtc aacgtggcca tttacgccat
     3181 tgctttgggc cagcggatcg gcattcccaa aaagcagttg tcccatctgg ggatggccgg
     3241 cctctttcat gacctcggca aggtcagcat ccccaaagaa attctcaaca agacggacaa
     3301 attatccccc caggagtggg cgatgattcg atcccatccc gtcgtggggg cggagatggt
     3361 gatccgtctc aaggagtggg gggagctctc ggcgcggctg atcgatgctt ccttcgagca
     3421 tcacttgaaa tacgacctga ccggttatcc caaactggcg cggaagagga gtatcaccct
     3481 cttcggcaag atcatcgcca tcgctgattt ctacgatgct ctggtgagac cccgaatcta
     3541 ccgtcaatac ccctatgtct ccgaaaagat tttgggcttg atgctggagc gggcggggaa
     3601 ggattttgac ccggcgctgg tgaaaatttt tatcaacatg atcgggatct ttcctctggg
     3661 gacgctggtc ctgctcaata ccaacgagat cggaatcgtg ttccagaccc aggaggaccc
     3721 tgagatgatc gaccggccca aagtctgcct gcttcaatac agcgatggag agtaccacaa
     3781 ggggaaggcc gtcgatttac gcgagatgga agaagggaca cggtccttca agaggtccat
     3841 tatgagaacc cttgacccga atgagtacaa catccacgta gccgagtttc tcctctaggc
     3901 tcctttcccc cgctggcttt ttttagatcg gatcttctcc gggttcccga ttattttact
     3961 accaaccccc cccggcccca tcgtttgatc tccgtgaggc tgacctcaaa gtcaaggggg
     4021 taggtgtaaa aaggaagctc aacctcctgg gtccaggaga cgatgagggt gagggaatcg
     4081 gaagtctgag tgatccgaac attcctgggg gtgagttgaa cctcttcact cctgacttcg
     4141 gagataaccc tttgcacaat ctccgcctca ggtttaggtc cgccggccac ggtccaggta
     4201 agagcttgcc gaacctgttc cctcacctgg tagtaagtcc agaaagcctc cccgaattta
     4261 aatcccaaaa aacctacgat gacgataagg atcagtaaaa ccaggcagcc cggagtcgtg
     4321 atccctacgt cattccgggt ccgggatcgg cttttctggt ggagaaaaaa tccggtcata
     4381 tctttttcat tcctttgttt tctgattttt ccatagggaa ttctcggaaa gaccagtatt
     4441 ttttgagttc tctctccttc tgggcataga ggctctggaa cctttcggga accaggaaag
     4501 aaagaccccg ggcgatggat aacgccgccg gagagatttt cgattccagg aggagcttgc
     4561 ttttgggcgg aaggaaggcg gtaagcacca gaagaatgat ggcgatgaga aggatggcct
     4621 tcaagacgcc gaaggccgcg ccccccattc ggtcggccca accgaggccc cctttatgaa
     4681 tcgccttgga gaggaggctt cccagaaaac tgactcccag ggcgacgagc aggaaaagga
     4741 cggcaaagct tacgatctgc gcaacggtct cctgttgcac ccaccccttc acccatgcgg
     4801 caacccgcga gtacccatag ctggctccga ggaatcccag aatgatggcc aggaaagaaa
     4861 aaacctctcg gatcagtccc cgaatgagac tgtaaagtcc tgaggctccg atgagaacca
     4921 gaaagataat gtccaaggta ttcatggatg gccttcttcg atttttatat agcagggacg
     4981 aaatttattc aagcccaaaa aagacgggca atccgttctc tcttaattcc ctcataaggt
     5041 ttcctatgat agggaggcag ggtctttcgg gtggaccctc gttttctccg accccttttt
     5101 tccccaggct ctcccgtctc cagggcaaga aaaaatagga atttcagttg cctcgaaagg
     5161 gggcttttgt tatttttaaa gaggaagatg ggtgcggttc tccttgaatt tttactcgtc
     5221 cggaggggtt tcttttccgc ggaaaggagt gcggtttgaa tcgcaaaagg gacctaggct
     5281 tcctttttaa tccccgatcc atcgctttgg taggcgtccc ccgcggcttt gagccgggaa
     5341 gggtttttct ccagggactt ctcgaccagg gctattcggg gaaggtcttt cccattaacc
     5401 ccaaggcgga ttccatcgat ggatgccgag cttacccctc ccttaaggaa gttccgggag
     5461 agatcgactt ggccatcctc cttttgccgg cagccctggt tttgccggca ctggaggagt
     5521 gcggcgaaaa aggagtgaaa atcgcagtca tctactcctc cggattcg
//
LOCUS       DASPRR010000230         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2148916, whole genome shotgun sequence.
ACCESSION   DASPRR010000230 DASPRR010000000
VERSION     DASPRR010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148916"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1097
                     /locus_tag="VEL68_08925"
     CDS             183..1097
                     /locus_tag="VEL68_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXZ36127.1"
                     /translation="MLGISSAWRSEILDSGREIVEEILNLGAQAMELEYRMTRSMLKE
                     VVPLVKEGRIAVTSLHNILPLPTWIPKNMANGEFVSLSSPDAKERKSALRYTLGTMEW
                     AEKFGARAIVLHMGKASMSGAMEFLMEMYDQGMTGTEEGRALIEGHKTTRAKLGKPLL
                     EAALKSLAILAKEGEKRGISWGIENRYKIQDFPNFEEFKTIFREFQGSSIRYWHDMGH
                     ATTQENLGLMPRGTLLENFGDILLGAHLHGCRGYKDHYAPGTGEENYSLLKRYLRPDT
                     IRVVETHHRATREELIQGLAFLREQGIT"
     gene            1130..2146
                     /gene="amrS"
                     /locus_tag="VEL68_08930"
     CDS             1130..2146
                     /gene="amrS"
                     /locus_tag="VEL68_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXZ36128.1"
                     /translation="MKEASLYESLPRHQARCQVCPRRCLLLEGKSGYCKTRANQGGKV
                     FSRIYGKVGSLAISPIEKKPMYHFYPGSLWLSLGSYGCNFRCPGCQNWELAHGDVEKE
                     AGRERVLSPDKLVEMALKGRCKGISWTYNEPTLWLEYIRDGAKLAKENGLLANCVTNG
                     YITNEALDFIGPFVDSFRMDLKGFSRGTYSCLAHIDDFSPVLKAAKGAKEKWGMQVEI
                     ITNVIPGINDSEDELGDIATWIGDHLGTDTPWHVTRFYPHFHLSHLPPTPVETLERGR
                     EIGFEAGLHFVYLGNVPGHDGENTYCPGCRKLLIRRHHFDVVENHLRGSLCPACGSKV
                     PGKF"
     gene            complement(2363..3064)
                     /locus_tag="VEL68_08935"
     CDS             complement(2363..3064)
                     /locus_tag="VEL68_08935"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HXZ36129.1"
                     /translation="MIDLHFKVFRFDPQADPGPYFKHYTVKAEPGERLLDSLNRIKWE
                     QDGTLSYRMSCAHGVCGSDGMKINGTCRLACQAIVKDYQCPEVVLEPLPGFKVLKDLV
                     VDIDPFLERIQNLRPFLIASAPPEKERKQTQAEGKKVEEAIRCILCACCTASCPVSWI
                     QKKYIGPAALVWAFRYIFESRDSDLQERLKQIDHPDGAWGCVNHFECTRVCPKEIPVT
                     KSINTIKREIERRLR"
     gene            complement(3064..>3470)
                     /locus_tag="VEL68_08940"
     CDS             complement(3064..>3470)
                     /locus_tag="VEL68_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HXZ36130.1"
                     /translation="KGEKVNAVRASLQKIMMEDCSVFRREEGLSHALKEIHDLQESYE
                     RIAVQDPGTRFNSDLMEAFELESLLGLAEAILASALARQESRGAHYREDFPERDDQNW
                     LKHTLILKADHGPRVFFKPVTITRFQPKARTY"
BASE COUNT          829 a          871 c          991 g          779 t
ORIGIN      
        1 agtaccagat gaaggaatat cgccccccga cgctggaaga aatggttggc gcttttcagg
       61 cggttggagg cgcgggcctt aaaaatgtga ggctgggaaa tacgggaatc tttgctcata
      121 ccgaagatca aattcaaacc ctacggcaaa aagtcggaat gggtcatttt taggggtggg
      181 agatgctggg aatatcgagt gcttggcgat cggaaatttt ggacagcggg agagagatcg
      241 tagaggagat attgaacctg ggggcgcagg cgatggaact ggagtaccgg atgacccgtt
      301 ccatgctgaa agaggttgtc cccctggtca aagaagggcg aattgccgtg accagccttc
      361 ataatatcct tcccctgccc acatggattc ccaagaatat ggccaacggc gagtttgtct
      421 ctttatcgtc gccggatgca aaagaacgga aatcagccct tcggtatacc ctgggaacaa
      481 tggaatgggc cgaaaaattc ggggctcgag ccatcgtcct tcatatgggc aaggcatcca
      541 tgagtggagc catggaattt ctgatggaga tgtacgacca ggggatgacc gggaccgaag
      601 agggaagagc cctgattgaa ggtcacaaaa caacccgggc aaagctggga aagcccctgt
      661 tggaggccgc cctgaaaagt ctggcaatcc tggccaaaga aggggagaag cgggggatca
      721 gctggggaat cgagaaccgt tacaagattc aagactttcc gaatttcgaa gaattcaaaa
      781 ccattttcag ggaattccaa ggaagttcta tccggtactg gcacgacatg ggacacgcca
      841 ccacccaaga aaacctggga ttgatgccca gaggaacgct ccttgaaaat ttcggcgata
      901 ttcttctcgg tgcccatctt cacggatgtc ggggctacaa ggatcactat gctcccggaa
      961 cgggggaaga gaattactct cttctgaaga gatacctaag gccggatacg attcgggtgg
     1021 tggaaacgca ccaccgggcc acacgggagg aactcattca gggattggcc ttcttgaggg
     1081 agcagggaat tacctgaagc gtttcttgga atggacatcc gggtgcaaaa tgaaggaagc
     1141 ctccctctac gaatcgctcc cccgtcatca agcccgttgt caggtctgcc cccgccgctg
     1201 ccttcttctg gagggaaaat cgggatactg taaaaccaga gccaatcagg gaggaaaagt
     1261 tttttcccgg atttatggga aagtcggttc cctggcaatc tcccccatcg aaaagaagcc
     1321 tatgtaccat ttctatcccg gtagcctctg gctttctctg gggagctatg gctgcaattt
     1381 ccgctgcccg ggatgccaga actgggagct ggcgcatggg gacgtggaaa aggaagcagg
     1441 aagggaaaga gtcttaagtc ccgataaact ggtggagatg gccctgaaag gaagatgcaa
     1501 ggggatctcc tggacctaca acgaacccac gctgtggtta gaatacatcc gggatggggc
     1561 aaaactcgca aaggaaaatg gcctattggc caactgtgtg accaatgggt atataacgaa
     1621 tgaggcgtta gacttcatag gcccttttgt ggattccttt cgtatggatt taaaaggttt
     1681 ttcccgggga acctacagtt gcctggccca tatcgatgat tttagtccgg ttttaaaggc
     1741 cgccaaaggg gccaaagaaa aatgggggat gcaagtggaa attatcacta acgtcatccc
     1801 ggggatcaac gattccgagg atgaactcgg cgacatcgcc acttggattg gggatcatct
     1861 cggcactgat accccctggc acgtgacccg tttctacccc cattttcatc tatcgcacct
     1921 gcctccgacc ccggttgaaa ccttggagag ggggagagaa atcggttttg aagccggcct
     1981 tcattttgtc tacttaggca acgttccagg ccacgacggg gaaaacacat actgcccggg
     2041 gtgtcggaag ttgctcattc ggaggcatca tttcgatgtc gtcgaaaatc atttgcgggg
     2101 aagcctttgc ccggcatgcg gaagcaaagt gccggggaaa ttttgagaac cctggctccg
     2161 gtttgcttgc atcgctagac ctacagattt aatccacggc ccttatcccc caaaaccctg
     2221 cgtgctctcc aagatttcta tcgggcaaag gcctgtccaa atcctatctc ggttgtcccg
     2281 gtggtaggga cggaggcgag tggggggctc ataggcaaca aatccgatcc gggaggggtg
     2341 agaggaagct gcgccaaagg ggctagcgca gtcttctttc gatttctcgc ttaatggtgt
     2401 tgatcgattt ggtgaccggg atctctttgg ggcagacccg ggtgcattcg aagtgattga
     2461 cgcagcccca ggctccatcg ggatggtcga tctgtttgag cctttcctga agatcagaat
     2521 ccctcgattc gaagatgtac cggaaagccc ataccagggc cgccggccct atgtattttt
     2581 tttgaatcca gcttaccgga caggaagcgg tgcagcaggc gcagaggatg cagcggatgg
     2641 cttcctccac ctttttcccc tccgcctgtg tctgcttcct ctctttttca gggggggcgg
     2701 aagcgatcag gaaggggcgg agattctgga ttctttccag gaagggatcg atatccacca
     2761 ccaggtcttt gaggaccttg aatcccggga gcggttcgag gacgacttcc gggcactgat
     2821 aatccttgac gatggcctgg caagccaggc gacaggtgcc gttgatcttc atcccgtccg
     2881 aaccgcagac cccatgggcg caggacatgc gatagctcag ggttccgtct tgttcccatt
     2941 tgatccggtt caagctgtcc aataaacgct ctccgggttc cgccttgaca gtgtaatgct
     3001 tgaaataggg cccggggtct gcctggggat cgaatcggaa taccttgaaa tgcagatcta
     3061 tcatcagtag gtcctcgctt taggttgaaa acgggtgatg gtcacgggct tgaagaagac
     3121 cctcggacca tgatcggcct taagaatcaa ggtgtgcttc aaccagtttt gatcgtccct
     3181 ttccggaaag tcctcccgat aatgggcgcc gcggctttcc tgacgcgcca gcgccgaagc
     3241 aaggatggct tcggcaagtc ccaggaggga ttccagttcg aaggcctcca ttaaatccga
     3301 gttgaaccga gtgcccgggt cctggacagc gatcctttca taactttcct gcaagtcgtg
     3361 tatttcctta agcgcgtggg acaatccttc ctcccgccgg aagaccgagc aatcctccat
     3421 catgatcttt tgcagggaag ccctcacggc gttaactttt tcgcccttgc
//
LOCUS       DASPRR010000231         9999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1666403, whole genome shotgun sequence.
ACCESSION   DASPRR010000231 DASPRR010000000
VERSION     DASPRR010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666403"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VEL68_08945"
     CDS             <1..199
                     /locus_tag="VEL68_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36131.1"
                     /translation="KLNNVRQAMKIARMARDILGGRGILADHHVIRHLCDLEAVSTLE
                     GTESMHTLIIGQEITGISAFA"
     gene            738..1352
                     /locus_tag="VEL68_08950"
     CDS             738..1352
                     /locus_tag="VEL68_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36132.1"
                     /translation="MKSMKRMLSWELVLLAAVSMFWVYTAPAWAGEGDMAKSSSMLSP
                     GSTNAPIAAPREGDVAKTSSFFRVDTAPAQADEGTMAQSACPETQTMNNRSVDMSGLL
                     GAMVLNKDHEALGRVIDVSGSASGGINFLIVSSCLPGMNGQLVAVPYTAYDYPSRWDS
                     VTLNLTTEDFKNAPSFSADSWPNGAGSDWSTKAYRYFENTQYFG"
     gene            1791..1934
                     /locus_tag="VEL68_08955"
     CDS             1791..1934
                     /locus_tag="VEL68_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36133.1"
                     /translation="MEEKSTYTDCELRYIRNAGYDPKQASEVCAQADEDRGKSSEEEI
                     WDE"
     gene            complement(2034..3326)
                     /locus_tag="VEL68_08960"
     CDS             complement(2034..3326)
                     /locus_tag="VEL68_08960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXZ36134.1"
                     /translation="MAFEFKLPDLGEGLTEGEIVKWLVKVGDSIEEGRAFVQLETDKA
                     VVEIPSPRRGTVLKLGAAEGSTVQVGQVLIVIGEPGEKLEAPPKPVKKPEKEEARPRP
                     SVGIVGELEEAEEEIEEKAPPKPAPAPPMERAEILAVPMVRKLAADLKIDLRMIKGSG
                     PQGRITKEDVLKEAKEKRPPEKEAPPKEIKELRKDAYGSVERIPLRGMRKTIALSMAK
                     SKSTIPHAAGIDEADVTQLLGLRAKAKERAAQRKIHLTILPFVIKAVVAALEEHPYLN
                     ASLDDGNGEILLKKYYNIGVAVDTKDGLMVPVVKNVKGKNIFQLATELTNLSDKARAR
                     TIDLTDLKGGTFTLTNFGAMGGLYGFPIIHHPEVAILGMGKIYEKPIVVDGKIEVRKV
                     LPLSLSFDHRVVDGAEAVRFMNTVIQLLEDPGLILLEE"
     gene            complement(3463..4440)
                     /locus_tag="VEL68_08965"
     CDS             complement(3463..4440)
                     /locus_tag="VEL68_08965"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXZ36135.1"
                     /translation="MAKRNLVEAINSGLIVEMERDSRVVVLGEDVGKEGGVFRVTDGL
                     QVKFGLNRVFDTPLAESGIIGVSIGMAVKGLRPVAEIQFEGFLHLSMNQIMNHAARIR
                     NRSRGRFTCPLVIRSPWGGGIHAPEHHSDSPEALFAHTPGLKVVIPSTPYDAKGLIIS
                     AIRDPDPVLFFEPKRLYRAIREEVPDGEYTVPIGKAKLVREGKDVTAITWGAMVREVL
                     RAAEAADKEGVKVEVIDLRSISPMDDETFLESVRKTGRAVVVHEAPPTCGVGAEVIAR
                     INEKALFSLQGPVVRVTGFDTIVPLTKLESHYLPSPERILAGIRKVMTF"
     gene            complement(4444..5523)
                     /gene="pdhA"
                     /locus_tag="VEL68_08970"
     CDS             complement(4444..5523)
                     /gene="pdhA"
                     /locus_tag="VEL68_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HXZ36136.1"
                     /translation="MIEKFDPLKGEMLRILHPDGRFEEALDPGLNDATVQTLYRDMVV
                     IRTADQRALTLQRQGRFGTYAPLIGQEAAQVGSAHALQKGDWVFPSFRETGVLYMMRA
                     PLSGLFVYWMGNEAGQKVPEDVNVFPISVPVGTHPLHAVGAAWAARLQGEKICTVAYF
                     GDGGTSEGDFHEAMNFAGVFQTPTIFFSQNNQYAISVPRKKQTASKTLAQKAVAYGFP
                     GIQVDGNDLFAVYAATKEARERAVSGQGPTFIEAVTYRFGPHTTADDPTKYRKDAEVE
                     EWKKLDPMLRLQKYLQGKGLWNEEIEAKIKAEAEKIVNQVVQDAEAVPPPDPREMFSF
                     TFAELTTRLKEQMDDYLTYLKEKEK"
     gene            complement(5659..6807)
                     /locus_tag="VEL68_08975"
     CDS             complement(5659..6807)
                     /locus_tag="VEL68_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36137.1"
                     /translation="MIGFDLTPGQKSMQEKARRFAQEVILPVAGKHDRDGSFPLDIMQ
                     KAYAEGFLTPLIPRQYGGGGWGMLDICLLSEELAAGDMGIFVSIFVSTLALYPIISFG
                     SEEQKERFLRPFCTKFTLGSYCLSEPAVGSDPASMETTAIPEGDAYLINGTKMWITNG
                     GFADLYLVFANTDRQKKYKGIISLIVPANLPGIRRGGPIDKMGQRASNTTAVTFKNVR
                     VGKENLLGGEAEGFKKAMAALDVTRPMIGAGAVGLARSAMELATQYAQKRIQFGVPIS
                     HHQAVQFILADMTIGIEAARLLVWRAASLADQGRRNSTESAVAKAFAADVAMRVTTDA
                     VQVYGAMGYTKYHPVEKLMRDAKIIQIYEGSAQIMRRIIARSMLGRVP"
     gene            complement(6804..7661)
                     /locus_tag="VEL68_08980"
     CDS             complement(6804..7661)
                     /locus_tag="VEL68_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXZ36138.1"
                     /translation="MDIKLIGVVGAGQMGGGIAEVAISSGFTVVMRDITPEFVQKGKL
                     RIASDLERRVQKGKMTAEEQKAVLGRLTTTTRLEEFKDCDLVIEAASEQIPIKKEVFQ
                     TLDAVTRKSAVLASNTSSISITRIASFTKRPDRVVGMHFFNPAPVMKLIELIRGMETS
                     EETFQTAREVSLKMGKTPWEAKDFPGFVSSRLIFNYMNEGVYALYEGVGTREEIDNIM
                     KMGANHPMGPIELADLVGLDTVLSVMKILNEAFGSRYQPCPLLTKYVEAGHLGVKTGR
                     GFYEYGGRK"
     gene            complement(7797..9170)
                     /locus_tag="VEL68_08985"
     CDS             complement(7797..9170)
                     /locus_tag="VEL68_08985"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXZ36139.1"
                     /translation="MDSMGKMKVPRQAYWGPETQRAYENFPISGLRFPRVFIRALGMI
                     KLAAAETNMRLGLLGLKPGRAVVRACREVIEGELDDQFVLDIFQTGSGTSTNMNANEV
                     IAHRANQTLGGKVGEKKPVHPNDHVNLGQSSNDVIPSAIHISALEGIQKDLLPALKSL
                     QAVLEKKAKEFDRVIKIGRTHLQDATPIRLGQEFGGYASMVEHGIQRLQMVRYHLAEL
                     AIGGTAVGTGLNTHRRFARLVIQKINRLTGLSFREAKNHFEAQGSKDAVVEASGAMKT
                     IAVSLMKFANDIRWLGSGPRCGIGEIRIPPVQPGSSIMPGKVNPVIPEALCQVCAQVM
                     GNDFTVTIAGMSGNFELNVMMPVMAYSLLQSIHLLSNTVAVFTHKCAMGLKADKERCQ
                     EMIEKSLAMVTALSPHIGHDRAAEIAREAFQTGKTVREIAMQSRVLPVKELEKILDPW
                     KMTEPGI"
     gene            9688..>9999
                     /locus_tag="VEL68_08990"
     CDS             9688..>9999
                     /locus_tag="VEL68_08990"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ36140.1"
                     /translation="MMSQTFQFKAPSVIVNGPGAAKEIGNFAKRIGKKALIVTDANLE
                     KIGLPKEVMKSLEIAGVPFAIFSRVVIEPTQDNVEEGVKAYKEAGVDFLIAVGGGSSM
                     DT"
BASE COUNT         2182 a         2737 c         2703 g         2377 t
ORIGIN      
        1 caagttgaat aatgtgcggc aggcgatgaa gatcgcccgg atggcccggg acatattggg
       61 gggtaggggg attctggccg atcatcacgt cattcggcac ctatgcgacc tggaagcggt
      121 gagcacgctg gaggggacgg agagcatgca caccctgatc atcggccagg aaataaccgg
      181 aatctcggcc ttcgcctgag ttttctgggg tccgccaatg actaggcgcg ggggttagtg
      241 gccggcagat ggataaactg cccggaacaa ttgctgcttc agtgcgaaaa ggaagataaa
      301 atcaaagggt tggaatcaaa ttgcttctca atctgcgcct gcaacccgcg gcgatgggct
      361 gtgcgcgaaa ccggttaaat ctgaggggga atcttttttt tcttcagggc atctccttgc
      421 tttagaaggg tgctgaatta cttgcagcga gggaaaaaaa tcggccaagg aaatgccaat
      481 taaggtgcgg ctgattctcc gcctcgagta taggcgaata attcgctatt gattctgcag
      541 cacccaggga aggaagaatt ttccgaaaag acggaaagat ttttcggtcc cgacgcgaga
      601 ggagctcgta aaaattttct ggaagagtcg ctccggagaa ggctggggtc cagtccctcc
      661 agaagtggga ttgaaattag aggatttcgg cttttttacg agctcattaa gatcgatcgg
      721 atgaaaggag agcaaacatg aaaagcatga aaagaatgtt gtcgtgggaa ttggttttgc
      781 tggcggcggt ctcgatgttc tgggtttata cagcgccagc atgggcaggt gaaggggaca
      841 tggccaagag ctcctcgatg ttatcgcctg ggtccaccaa tgcaccgata gccgcgcccc
      901 gtgaagggga cgtggccaag acctcctcgt ttttccgggt tgatacagcg ccagcacagg
      961 cagacgaagg gacgatggcc cagagcgcgt gcccggagac tcagaccatg aacaataggt
     1021 cagttgacat gagtggcctg ctgggtgcta tggttttgaa caaagaccat gaggcgttgg
     1081 ggagagttat cgatgtatcg ggttccgcca gcgggggaat taacttcctg atcgtttcct
     1141 cttgcttgcc cgggatgaat gggcagctcg tcgcagttcc ctacacggcg tatgactatc
     1201 cgtcaagatg ggacagtgtg accttaaacc tgaccacgga agatttcaag aatgctccca
     1261 gtttttctgc ggactcgtgg ccaaatgggg cgggtagtga ttggagtaca aaagcttacc
     1321 ggtattttga aaatacccaa tattttgggt aaacttccag gtgagcgaaa acagaggtcg
     1381 tgaacgaaaa ggcggagtaa gtaaggcgcg gtggataaat agatcaccga cgccttcctc
     1441 ctccctgtga aaccggtttt tatttagcat atccagcctc agtcaccact tatagccctc
     1501 ttccgaagct gattttcagc tagcaatcaa agaaaccaaa gcggaacgta atcgctgagg
     1561 attcgcctgc cgggctaatt tgcggctatc ctttttacca attggggggc tcattcccca
     1621 aagccagtga tggtagtgat ttccgaaagt ccccccattc aaaggaaagg cgatccagcc
     1681 ctgaaaccct gggaaaaatc ttttttttga aaaaggcatc taaatttcgg atgttggggt
     1741 gttttcaagg cgcggaaaga gacgaattca aagatgagga gggattcacc atggaagaga
     1801 aatcgactta tacggattgt gaattgaggt atattcggaa cgcaggttat gatccaaagc
     1861 aggcctctga agtttgcgcc caagccgatg aggatagagg gaagtcaagc gaagaggaaa
     1921 tctgggatga ataaggcggg gaccaatagc gaaggcatgg tttaaagggc tccggggcgg
     1981 gacctctttg agccctggta ccattcaggt acatctcgtt gggagtgatc cccttattcc
     2041 tccaacaaaa tgagacccgg gtcttcgagg agttggatga ctgtgttcat gaaccggacc
     2101 gcctcggctc cgtccaccac ccggtggtca aaagagaggg agaggggcag aacctttcgc
     2161 acttcgattt ttccgtccac gacgatgggc ttttcataaa tctttcccat ccccaagatg
     2221 gccacctcgg gatggtgaat aatgggaaag ccgtaaagac cgcccatagc cccgaaattc
     2281 gtgagggtga aagtcccccc ctttaaatcg gtcaggtcga tggtccgggc gcgggcctta
     2341 tccgaaaggt tcgttaattc cgtggccagc tgaaagatat tcttcccttt gacgttcttc
     2401 accaccggca ccatcagtcc atctttcgta tccacggcga cgccgatgtt gtagtatttc
     2461 ttcaggagga tctctccgtt gccatcatcc agggaggcat tgaggtaggg gtgttcttcc
     2521 agggctgcga ctacggcctt gattacaaag ggaaggatgg tgaggtgaat tttcctctgg
     2581 gccgccctct ccttggcctt ggccctcaaa cccaagagtt gagtcacgtc ggcctcgtca
     2641 attccggcgg catggggaat ggtggatttg gatttggcca tggatagggc gatggtcttg
     2701 cgcattcccc gaagcggaat tctttcgacg gaaccgtaag cgtccttacg gagttctttg
     2761 atttcttttg gcggcgcctc tttctccggt ggccttttct cctttgcctc tttgaggaca
     2821 tcctccttgg tgatgcgtcc ctgaggcccc gaccccttga tcatcctcag gtcgattttt
     2881 aaatctgcgg caagtttcct taccatgggc acagccagga tttcagcccg ctccattgga
     2941 ggggctgggg ccggcttggg cggagccttc tcttcgatct cttcttcggc ttcctccagt
     3001 tctcccacga tgcccactga gggacgtggc ctggcctctt ccttctcggg ttttttcacg
     3061 ggcttgggtg gggcttctaa tttctccccc ggttcgccga tcacgatgag cacctgccct
     3121 acttgaacgg tcgacccctc ggccgccccc agtttaagga cggtccctct ccgcggcgaa
     3181 ggaatctcca ccaccgcttt gtccgtctcc aactggacga aagcccggcc ctcctcgatg
     3241 gaatcaccga ctttgaccaa ccatttgaca atttcgccct cggtcaatcc ttcgcctaaa
     3301 tccgggagct tgaattcaaa ggccattcaa acctcctcaa gatagtttcg agttttgggt
     3361 ttcaggttcc gagtttctaa ttttatatca tgggtctcgg gtctcagttc ccaaaatttt
     3421 tctttaaccc gaaactcgaa gcccgaaaac cgaaacaatt tttcaaaaag tcatcacctt
     3481 ccggatcccc gccaggatcc gctctgggct tggcagatag tggctttcca gcttggtcag
     3541 gggaacgatg gtatcaaacc cggtcactct gaccacgggc ccttgtagcg aaaacagggc
     3601 cttctcgttt atcctggcaa tgacctccgc ccccactccg caggtcgggg gagcctcatg
     3661 gactacgacc gccctccccg tttttcggac agattccaaa aaggtttcat catccatggg
     3721 ggagatgctt ctcaggtcga tgacttcaac tttgactcct tccttatcag cggcctccgc
     3781 cgcccggagg acctcccgca ccatggcccc ccaagtgatg gccgtaacgt ccttgccctc
     3841 ccggaccaac ttggcctttc cgatgggaac ggtgtactcc ccgtccggga cttcctcccg
     3901 gatggcccgg tagaggcgtt tgggctcgaa aaataaaacc gggtcggggt cccggatcgc
     3961 cgagatgatc agacctttgg catcgtaggg ggtggaggga atgaccactt tcaatcccgg
     4021 agtgtgggca aaaagggctt cggggctgtc ggagtgatgt tcgggggcat ggattccgcc
     4081 gccccagggc gagcggatca ccagggggca ggtaaagcgg cctctggacc gattccggat
     4141 tctcgccgca tggttcatga tctggttcat ggatagatga agaaagcctt cgaattgaat
     4201 ctcggccacg ggcctgaggc ccttaaccgc catcccgatg gataccccga tgatgcccga
     4261 ttcagccaaa ggagtgtcga acacccggtt caatccgaat ttgacctgga gcccgtcggt
     4321 gacccggaaa accccgcctt ctttccccac gtcctcaccc aaaactacga cccgggaatc
     4381 tctttccatc tctacgatta gaccgctgtt gatggcttcc accaggttgc gtttggccat
     4441 ccttcatttc tccttttctt tgaggtaagt caggtaatca tccatctgtt ctttgaggcg
     4501 cgtcgtcaac tcggcgaagg taaaggaaaa catctccctg gggtccggag gcggaactgc
     4561 ctccgcatcc tgtaccacct ggttcacgat cttctcagct tcggccttaa ttttggcttc
     4621 gatctcttcg ttccaaaggc ctttcccctg gagatacttc tgcaacctca gcatggggtc
     4681 cagtttcttc cattcctcga cctcggcatc tttccggtat ttggtgggat catcggccgt
     4741 ggtgtgaggg ccaaaacggt aggtaaccgc ttcaatgaag gtgggcccct gaccggagac
     4801 cgcccgctct ctcgcctcct tcgtagcggc atagacggcg aacaaatcat tcccgtctac
     4861 ctggattccg ggaaagccgt aagcgacggc tttttgcgcc aaagttttcg aagcggtctg
     4921 ttttttccgg gggacggaga tggcgtattg gttgttctga gaaaagaaga tcgtcggagt
     4981 ctgaaagact ccagcgaagt tcatagcctc atgaaaatcc ccctccgaag tgcccccatc
     5041 cccgaagtag gccacggtgc agattttttc gccctgaagc ctggcggccc aggctgcccc
     5101 caccgcatgg agggggtggg tgcctaccgg gacggatatg ggaaacacgt tgacgtcttc
     5161 agggactttc tgtcccgctt cattccccat ccagtaaaca aagaggccgc ttagaggagc
     5221 cctcatcata tagaggacgc cggtttcccg gaatgagggg aatacccaat cccctttttg
     5281 cagggcatgg gcgctaccca cctgggcagc ctcctgcccg ataagcgggg cgtaggttcc
     5341 gaaccttccc tggcgctgca gagtcaaggc cctctggtcg gccgtacgaa tgacgaccat
     5401 atcccggtaa agtgtctgca ccgtcgcatc gttcagaccc gggtcgaggg cttcctcgaa
     5461 tcgcccatcg gggtgaagaa tcctcagcat ctcccctttc aaaggatcaa atttttcgat
     5521 cattgtgtct ccttagagct ttatcccaat gacgcgtagc cgcgcgccgc tacaaaccta
     5581 ttcagtgtga aagctaattt ttcaacttga ggtatttggg cacgcttgag gcactgtatt
     5641 ttactctatt ttcgtccctc agggaaccct tcccagcatg gaacgggcga tgatccggcg
     5701 cataatctgg gccgaccctt cgtagatctg gatgatttta gcgtcgcgca tcagtttctc
     5761 cacgggatga tactttgtat agcccatggc tccatagacc tgtacggcgt cggtggtgac
     5821 ccgcatggcc acgtccgcag caaaggcctt ggccactgca gactcggtcg aattccgcct
     5881 gccctgatca gccagagacg ccgcccgcca gactaggagc cgggcggctt cgatgccgat
     5941 ggtcatgtcg gccagaatga actggacggc ttggtggtgg gaaatgggga ctccgaactg
     6001 gattcgcttc tgggcgtact gggtcgccag ttccatagcg gaccgagcca atcccacggc
     6061 cccagctccg atcatgggac gggtgacgtc cagggcggcc atggctttct taaatccttc
     6121 cgcttctccc cccaggaggt tttccttgcc tacccgcacg ttcttgaaag tcacggccgt
     6181 agtgttggac gctctctggc ccatcttgtc aatcgggccg cccctccgga tcccgggtaa
     6241 attggctggg acgatcagag agatgatccc cttatacttc ttctgccggt cggtattggc
     6301 gaagaccagg tacaagtcgg caaacccccc gttggtgatc cacatcttgg tgccgttgat
     6361 aaggtaagca tctccttctg gaatggcggt cgtttccatc gatgcagggt ctgatcccac
     6421 tgcgggttcg ctcagacagt aggaacccag ggtgaacttg gtgcagaaag ggcgcaagaa
     6481 acgttccttc tgctcctcgc ttccgaagga gatgatggga taaagagcca gggtgcttac
     6541 aaaaatggaa acgaaaattc ccatgtcccc tgcggccaat tcttcggaca gcaggcaaat
     6601 gtccagcatg ccccaccctc ctcccccgta ttgccttggg atgagggggg tgagaaaacc
     6661 ttcggcgtac gccttctgca tgatatcgag gggaaaggag ccgtcgcggt catgtttacc
     6721 cgccaccggg aggatgacct cctgggcaaa gcgtcgggcc ttttcctgca tacttttctg
     6781 acccggggtc agatcaaatc cgatcatttc ctccctccat attcgtagaa tcctctgccg
     6841 gttttgactc ctagatggcc cgcctccacg tacttggtga gaagggggca aggctgatac
     6901 cgggacccga aggcttcatt tagaatcttc atcaccgaaa gcaccgtatc caaccccact
     6961 aggtcagcca actcaatggg ccccatgggg tgattggccc ccattttcat gatattgtcg
     7021 atttcttccc gggtcccaac cccttcgtaa agagcatata ccccttcatt catgtagttg
     7081 aagatcaagc ggctggagac gaacccggga aaatccttcg cttcccaagg ggtcttgccc
     7141 attttcaggc tgacctccct ggccgtttga aaagtctcct cggaggtttc catcccccgg
     7201 atgagttcga tcagtttcat caccggcgcc gggttaaaga agtgcatgcc caccacgcgg
     7261 tccggtctct tggtgaagga ggcaatgcgg gtgatagaaa tggacgaggt gttgctggcc
     7321 agtactgcgc tttttcgggt caccgcgtcg agggtctgga atacctcttt tttaatggga
     7381 atctgttcgc tggccgcttc gatcaccagg tcgcaatcct tgaactcttc cagccgggtg
     7441 gttgtagtca gccggccgag aacggccttc tgctcttccg cggtcatttt ccccttttgc
     7501 acgcgtctct ccaggtcgga ggcaatccgc aacttcccct tttgcacaaa ctccggagtg
     7561 atgtcgcgca tcaccacggt gaaaccggag gagatagcca cctcggcaat gcccccaccc
     7621 atctgcccgg cgccgacgac gccgatcaat ttgatatcca ttgaacacct ccccctaaga
     7681 tgcctaaaat caaaaagcca gaagtcagga gtcagactaa ggttttgttc ttttggcttc
     7741 tggctcctga attctgttct cccaattccc aattcgcaat tggcaattcg aaatatctat
     7801 attcctggtt cggtcatctt ccatgggtcg agaatcttct ccagctcttt gaccggaagg
     7861 acacggcttt gcatggcgat ctcccggaca gtcttgccgg tctggaaggc ttcccgagcg
     7921 atttcggcgg cccgatcatg gccgatgtgc gggctcaggg cggtaaccat ggccaggctc
     7981 ttctcgatca tctcttgaca cctttccttg tcggccttca gacccatcgc acacttatgc
     8041 gtgaacacgg ctacagtatt ggacagcaag tgaatggatt ggagaagact gtaagccatc
     8101 actggcatca tgacattcag ctcaaaattg ccggacatcc cagcaattgt caccgtgaaa
     8161 tcgtttccca taacctgggc gcaaacctgg catagggcct cgggaatcac ggggtttacc
     8221 ttcccgggca tgatggacga ccccggctgc accgggggga ttctgatttc cccgatcccg
     8281 caacggggac ctgaccccag ccagcgaata tcgttggcga acttcattag gctaacggcg
     8341 atggttttca ttgctccgct ggcctccacc acggcgtcct tcgatccctg ggcctcaaaa
     8401 tgattcttcg cctcgcggaa agacaacccg gtcaatcgat tgatcttctg gatcaccagc
     8461 cgggcaaatc gacgatgggt gttgagaccg gttcccactg ctgtcccgcc gatggccagt
     8521 tcagcgagat ggtagcggac catctgcagc ctttgaattc catgttccac catactggcg
     8581 taaccgccga actcctggcc caggcggatg ggagttgcgt cttgaaggtg ggtgcggccg
     8641 atcttgatga cccggtcaaa ttccttagcc ttcttctcca gcaccgcctg tagggatttc
     8701 agggctggga ggaggtcttt ttgaattcct tccagtgcgg aaatatgaat ggcgctcgga
     8761 attacgtcat tgctcgactg gcccaggttt acatggtcgt tggggtggac cggcttcttt
     8821 tccccgacct ttccccccag ggtctggttg gccctatggg cgatgacctc gttggcgttc
     8881 atgttggtcg aagttccgga accggtctgg aagatatcca ggacaaattg gtcatcgagc
     8941 tcgccctcta tgacctcccg acaggcacgc actacagccc ggccaggttt gagacccaaa
     9001 aggccgagcc gcatattggt ttccgcagcg gctagtttga tcattcccag tgccctgatg
     9061 aacacccgcg gaaaccggag gccgctgatg ggaaaattct cataagccct ctgggtttca
     9121 ggcccccagt aggcctgtcg gggaaccttc atctttccca tcgaatccat ctcaacacga
     9181 aatcctccgg gcttcatttt tcaggttcct ttcggtagga cttcaaaagc tgtttggctc
     9241 ttggctattg cgataagacg atgatttcaa taataaaatg gatgaatcaa agaaagaaat
     9301 aaatacagga aaccgtgtat tttgggccgg gaaacactgt attttcagca agggggaggg
     9361 ccaagggacc tccgaaagga ccaggaagtg gaaacggcga aatgattgtt gaattatcag
     9421 ttgaatgaat ccccgatagc ggatatattc aatatgccca agaagaacag aggtggatat
     9481 ttcaagggcg atcatgtccc tgaaatcccc cccaaccccc cctttaaaaa aggggggcag
     9541 gggggatttc tggcagggct acgcttccag gaaaaatcct ggctaccagt tgcggcctga
     9601 tcattctgcg aaccgctttt ggaaactcat tcgtcaccta cttttcgagc atagctcaat
     9661 ctgatgttgt aaaaggagaa aaatattatg atgtctcaaa cgtttcagtt caaggctccc
     9721 tcggtgatcg tgaatggtcc cggtgcggca aaagaaatcg gaaacttcgc caaacgaatc
     9781 ggcaagaaag ccctcatcgt caccgatgcc aacctggaga agatcggact ccccaaggag
     9841 gtaatgaaat cgctggaaat tgccggggtt cccttcgcca ttttcagcag ggttgtaatc
     9901 gagcccaccc aggacaatgt cgaagaaggt gtgaaggcct acaaggaggc cggggttgac
     9961 ttcttaatcg ccgtaggggg cggaagctcg atggatacg
//
LOCUS       DASPRR010000232         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1181183, whole genome shotgun sequence.
ACCESSION   DASPRR010000232 DASPRR010000000
VERSION     DASPRR010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181183"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VEL68_08995"
     CDS             <1..205
                     /locus_tag="VEL68_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HXZ36141.1"
                     /translation="ESGRVKEIFAAGTAAVISPVGKVAYKGKNYVINQGKVGPLARKL
                     YDTIMGIQYGKIKDPYGWMMRVT"
     gene            342..1241
                     /locus_tag="VEL68_09000"
     CDS             342..1241
                     /locus_tag="VEL68_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXZ36142.1"
                     /translation="MPLKVKKIAVIGTGTLGTQIAVHSTYHGYDVSAHDADPKSLEKV
                     LQMIQVRIANSNRKPTIPLKEIQRQAKKIKMLATIEGAVWEADLVIEAIPEDLMLKRR
                     IFKQIDFYSPKKAILATNSSSIPISRIENATRRPERCLNLHFYSLDLGRNIADVMGGT
                     KTTRQVLETGKEWVRSLGCVPLTVNRELLGFCFNRVWRAVKRETLHMWAGGYVDFRDI
                     DRGWMIWTGMSQGPFGIMDAVGLDVVYGIEMVYYNESKDRRDFPPAALKKMIERNQLG
                     VKTGKGFYTYPNPEFKSPNFLKP"
     gene            1408..1719
                     /locus_tag="VEL68_09005"
     CDS             1408..1719
                     /locus_tag="VEL68_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HXZ36143.1"
                     /translation="MATVLFMVKATIPKDKEAAFNRWYNEVHVPMVLQFNGMVSARRY
                     KAILGEEKYQYIAMYEIKDEATFHKLMESDHMKALRADYDAKFGSVSERARLAYVQVF
                     P"
     gene            1947..3440
                     /locus_tag="VEL68_09010"
     CDS             1947..3440
                     /locus_tag="VEL68_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017905185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXZ36144.1"
                     /translation="MPDYDVVVIGAGNAGLTSALTLAKNGAKVLLLERHNIPGGCATS
                     FIRGRFEFEVSLHQLSGIGNEKKPGPLRLLLGELGILDKIELVEMKDLYRLVIPGKID
                     IILKAEREAVLEALKKRFPNEGPSIDRFFDFLYKFASEMVGVAFARDPAASRGKYPLF
                     FKYALKPTQQVLDEYLQDPLLKSIITTYWSYLGLPPSLLPFADFAVMLWAYIEFKPYH
                     IKGGSQSLSNALLDSFLQAGGTARFNCAAQKISASGGKIRGVWTEDGEEVSCSVVLSN
                     ASTLLTYTDLLGAENLPPESFQTFAGKTVGPSGFTVFLGFDCEPADLGIAETNNFISR
                     QPDPAVSFACWRTLDAPDAVALSCYDVSDPDFSPPGACQAALVTLQYAEPWLSLPPSK
                     YSEVKYRIAREMIDLARQVFPKITHHLEEVEVGTPLTHMRYLGHPGGAIYGFDQYAKD
                     SYFFEDRRSPVQGLYFAGSWVAPGGYQTTLQSGVSAARAILKSMSKK"
     gene            3449..>3747
                     /locus_tag="VEL68_09015"
     CDS             3449..>3747
                     /locus_tag="VEL68_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36145.1"
                     /translation="MSRDFRPEIEGYGEIRREIEVLRRYGFDYSSHKGRVAQVLNLLH
                     PKKIGLQVSEVRKETQTAKSFRLVSPDGYLPPFQAGQYINLFVDVGGVRTSRPYS"
BASE COUNT          913 a          998 c         1007 g          829 t
ORIGIN      
        1 ggaatccgga cgggtgaagg agatctttgc cgccgggacg gccgcggtca tttcccctgt
       61 gggaaaggtg gcctacaaag ggaaaaatta cgtgatcaac cagggaaagg taggtcctct
      121 ggcgcggaag ctctacgata ctatcatggg catccaatat ggaaagataa aagaccccta
      181 tggatggatg atgagggtga cctgattcca ccagaggcga ggcggacaat gattcccccg
      241 aaaaagccaa cattcttctc ggtgttggct ttttaatttc aaaggttaca tatcgaaacc
      301 tgaaagaaga ctttaagtca atttgaaccc ggagaaacaa aatgccccta aaggtcaaaa
      361 aaatcgccgt gatcggaacc ggcaccctgg gaacccagat tgccgtccac tccacctacc
      421 atgggtatga tgtatccgcc catgatgcgg atcccaaatc attggaaaag gtcctgcaga
      481 tgattcaagt gcggatcgcc aattccaacc gaaagccgac catccccctg aaggagattc
      541 agaggcaggc aaagaaaatc aaaatgctgg caaccattga aggggcggtg tgggaagccg
      601 acctggtaat tgaggcgatt cctgaggatt tgatgctgaa gaggcggatt tttaaacaaa
      661 ttgattttta ttctcccaag aaggcgatct tggccaccaa cagctcctcc attcccatat
      721 ccagaattga gaatgcgacg cgccggccag agaggtgtct gaatcttcat ttttattccc
      781 ttgacctggg gaggaatatc gccgacgtca tgggaggaac gaaaacgacc cggcaggtgt
      841 tagaaaccgg gaaggaatgg gttcgatccc tcggctgcgt tcctttaacc gtcaacagag
      901 aactcctggg gttctgcttc aaccgagtct ggagggcggt caaacgggag actcttcaca
      961 tgtgggccgg agggtacgtg gacttcaggg atatcgaccg tgggtggatg atctggacgg
     1021 gcatgtccca gggccccttc ggaatcatgg acgcagtagg actcgatgtg gtatacggca
     1081 tagaaatggt ttattataac gagtcgaaag accgcagaga ctttccgcca gctgccttga
     1141 aaaagatgat tgagcggaat caactggggg tgaagaccgg gaagggcttc tacacctacc
     1201 caaacccgga attcaaaagc cccaattttt taaagcccta atttcaccac agagacagag
     1261 ggcggcagag ccgcgatcca aaggaatcgc ctcccctcct cctcttcgag gggggggatg
     1321 ttttatgccg aatgggaaat tcagaaattg aacatacaga gaaaaactct ttgtttggtg
     1381 aaaaattttt tttaaaagga ggagaatatg gcaaccgtat tgtttatggt caaagcaacg
     1441 atccccaagg ataaggaagc ggccttcaat cgttggtaca acgaagtcca tgttcccatg
     1501 gttcttcagt tcaacggaat ggtcagtgcc cgccgctaca aggcgatttt gggggaagaa
     1561 aagtatcaat acatcgccat gtacgagatc aaggacgagg cgaccttcca caagctgatg
     1621 gagtcagatc acatgaaagc gctgagggct gactatgacg ccaaattcgg ctcggtgagc
     1681 gaacgcgccc ggttggccta cgtccaggtc ttcccgtaag gcttttttct ataaaggtcc
     1741 tgcaaaactc tcgaaaacgg ggaggttgtc attccgggca agcgaagcgc gacccggaat
     1801 ccaggaattt caaaaaattc tagattccgg ctttcgccag gaatgacgga gtaagtaact
     1861 tttgtagaag ccgcctttgc tttactattc ctttgcttct ccccaaatga cggcggtgat
     1921 agccattctc ctgcaaggag gaacccatgc ctgattacga cgtggtggtc atcggcgcag
     1981 gaaacgcggg gctgacctcg gccctcaccc tggccaaaaa cggagccaag gttctgcttc
     2041 tggaaagaca caacatcccg gggggatgcg ccacgagctt catccggggg aggtttgaat
     2101 tcgaggtttc cctgcaccag ctgagcggaa tcggcaatga gaagaagccg gggcccctgc
     2161 gcctgctcct gggcgagctg ggtatcctgg ataaaatcga gctggtggag atgaaggacc
     2221 tataccgtct ggtgatcccg gggaagatcg acattatctt gaaagcagag cgggaggccg
     2281 tcttggaagc gctgaagaag cggtttccga atgaaggccc ctccatcgac cggttctttg
     2341 atttcctcta taaattcgct tccgagatgg tcggtgtagc ctttgcccgc gaccccgccg
     2401 cctcccgggg aaaataccct ctctttttta aatacgccct taaacctacc cagcaggtct
     2461 tggatgaata cctgcaggac ccgctcctga agtcgatcat aaccacttac tggagctatc
     2521 tgggcctgcc cccttccctt ctgccctttg ccgactttgc cgtaatgctc tgggcttaca
     2581 tcgagttcaa gccataccat atcaaggggg gctcccagtc tctttccaat gcccttctgg
     2641 attctttttt gcaggccggc ggaaccgctc ggttcaactg cgccgcccag aagatttcag
     2701 cttccggagg aaaaatcagg ggggtgtgga ccgaggacgg cgaggaagta tcctgcagtg
     2761 ttgtgctttc caacgcatct accctcttga cctatacaga cttgctcggc gcagaaaacc
     2821 tgcccccaga aagttttcag acctttgcgg gaaagaccgt tggcccgtct ggctttaccg
     2881 tcttcctcgg gttcgactgt gagcccgcgg acctggggat cgcggaaacc aacaatttca
     2941 tcagccggca gccggacccc gcggtatcct tcgcctgctg gcggactctg gatgcccccg
     3001 atgcagtcgc cctttcctgc tatgacgtat ccgatcccga tttctcccct cccggagcct
     3061 gccaggcagc cctggttacc ctccagtacg cagagccttg gctttccctt cctccctcca
     3121 agtactcgga ggtcaaatac cgcatcgccc gagaaatgat cgacctcgcc aggcaggtct
     3181 ttccgaaaat cactcaccac ctggaagaag ttgaggtggg gactcccctg acccacatgc
     3241 gttacctcgg ccatccggga ggagctatct acgggttcga ccaatatgcc aaggactcct
     3301 attttttcga agaccgccgc tcgcccgttc agggccttta ctttgccggc tcctgggtgg
     3361 ccccgggtgg gtaccagacc acgctccagt cgggcgtctc cgccgcccgg gccattctga
     3421 aatccatgag caagaaatga ggtgaggtat gagccgagat ttcagaccag aaatcgaagg
     3481 gtatggagag attcggcggg agatcgaagt tctgcgaagg tacgggttcg actattcatc
     3541 ccacaaagga agagtggcac aggttctcaa tctcctccac cctaaaaaaa ttggcttgca
     3601 ggtttccgaa gtccggaaag agacccaaac tgccaagtca tttcgtttgg tctccccgga
     3661 tggctatctt ccccccttcc aggccgggca atacatcaat ctttttgtcg atgtgggtgg
     3721 agtcaggacc agcaggcctt acagtat
//
LOCUS       DASPRR010000233        11052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1501683, whole genome shotgun sequence.
ACCESSION   DASPRR010000233 DASPRR010000000
VERSION     DASPRR010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501683"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1194)
                     /locus_tag="VEL68_09020"
     CDS             complement(238..1194)
                     /locus_tag="VEL68_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ36146.1"
                     /translation="MLTRREFVVGAVAAGVILRTQTVFAKPSQPMTQVNFEVPPGACD
                     CHTHIHGDPAQFPFFAGRVYTPEMALPEEMARLHKALHIQRVVIVTPSVYGVDNSATL
                     WGIKARGNDGRGVAVISDKTPDSELDALGQAGIRGIRLNLKTVGITDPAVAVQMFQAS
                     AERMKKRNWHIQLFASMEMIQAIKAQLTASPVPLVFDHFAGAEPEKSVEQPGFAGILD
                     LVRAGKAYVKISIPEGPRHDYSGFIPLAKALIAANPDRILWGTNWPHPNSMPPAGRKP
                     TEVTPLYQVDDGLVLNLLPVWAPDPAVRKKILVDNPARLYGF"
     gene            1784..2176
                     /locus_tag="VEL68_09025"
     CDS             1784..2176
                     /locus_tag="VEL68_09025"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXZ36147.1"
                     /translation="MKKEIIVSQKLSKPLGIYSQAVKAEGSRLLFISGMTARDKDGNI
                     VGKGDMKAQTRQVLENIKAVLERVGVTFDQIVKVTVYVTDMSRFKEIHEVRAQYFKKD
                     YPASTLVQVSGLVSPEMLIEIEAVAVLD"
     gene            complement(2250..5420)
                     /locus_tag="VEL68_09030"
     CDS             complement(2250..5420)
                     /locus_tag="VEL68_09030"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXZ36148.1"
                     /translation="MAKIQKYLPQGLTEKILAQKNRIEGERKQVTVLFCDIVGFTALS
                     EMLGHEEVYSLMDQVFEVLIHQVHAFEGTVNKMTGDGIMALFGAPIALEDAPQRALRS
                     ALAISRELSKFTERRKQERGIPPIQMRIGIHTGPVVVGSLGSDLRVEFSAVGDTVNLA
                     SRMEGMADPGTILVSEETYRLTEGLFWFEFAGEKKVKGKEKPVKTYRVIAPSSSKTRF
                     EVSAGKGLTSFVGREREIELLKDGFSRAKEGRGQVISIVAEAGLGKSRLLYEFRKAVA
                     EEEGVILEGRCLSYGRAMAYHPFIEVLKATFDVREGDEESEIKRKVIEGQKNLEMEES
                     LYLPFLLELLSVKDSGIEKILISPDARKDRIMEAFKRILLKMAAARALILIFEDLHWM
                     DKSSEETLLSFLEMIPGARILAFFTYRPEFIPSWNAKSYHSQITLNRLSNRETLAMVA
                     HLLGTRVLSRDLEELILEKTEGVPLFIEEFLRSLRELKIMVRRNHHYQLARNIDALTV
                     PSTIQEVIMARVDSLPEGARDLFQTASVIDREFSHELIKRASDLPEPELLSRLSVLKN
                     AELLYERDIYPRSTYVFKHALTREVVYDSLLSRRKKQLHERIGQVMEQLYRNNLDEHT
                     DVLAEHFIKSENFEKGAEFAKLAGKKARKRSAYGDAISYTYKELFCLEKMPNTPAVQQ
                     RILDARVALANYCMNLNDHVEAYEVVSPIADLALRINYRRGLPSIYTAIGSYLLFVEE
                     DYVQGMEYMVQALEFSEENKDFFSLWNACYIMGTALSWNCDFQKSLDYFQKSLDLSMA
                     ARSSMGISSAKINTGVNYLFQGKVEQAHQVSREALDIGEACGDIYIKGMAHSCYGTAC
                     YYRGLFEVAEVYLLRGIGFCENTSHFTWGSWASAFLGDMYFDLGRYEKSVESYQKAFS
                     FLEKKRFGPSWLTLIRVAIARTRVLQGGSEISLDEVLKSFSQNKNRAFSGWIAQYTAE
                     ILFHLSEQFLCQAREWADKAVGLDRQNGMQLLLGRDYLLSARIWNRMGHPARSREDLF
                     RAIEIFQQCGAQGYLKKASQELALIS"
     gene            6068..6421
                     /locus_tag="VEL68_09035"
     CDS             6068..6421
                     /locus_tag="VEL68_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HXZ36149.1"
                     /translation="MRRFVIEVGMGVDQHGQDNTRAAEKAVQDAIHRSCLCGLREVAG
                     IKSSDEMMVEVLVGCPAPETVDKERVLKVLPLGQKRIEVRKGGLSGKTLFQPELGDKT
                     DEMVVANAIITVYVP"
     gene            6568..7101
                     /locus_tag="VEL68_09040"
     CDS             6568..7101
                     /locus_tag="VEL68_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ36150.1"
                     /translation="MKSIFVNPERCTACRTCEIACAVHRSSLSRRMPEAIYEAVPPLP
                     RVRVEGTGTEKGFPIQCRHCEDAPCLDACPAGALFRDEGGLVLVHDERCIGCWMCIMV
                     CPFGAPQPFRHFRKVLKCDQCKGMDTAYCVESCPTHALMLIDPEDIARGKFKPPRKGS
                     LVGLNFAARSALFKQDK"
     gene            7194..8756
                     /locus_tag="VEL68_09045"
     CDS             7194..8756
                     /locus_tag="VEL68_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36151.1"
                     /translation="MNRTHKKSALAQTSSLILALHDQGEDLSWGRYEEQLPLCAFTSN
                     GLNCRKCFQGPCRINPFGDQPTRGVCGADREQIVMENLFQATLEGVLETARSVSSIDE
                     SLGGQEIPDLDSDLPQETRKRLAEHELLPVRKTHLLEVQNSYFSHKGYLSRTLRDLTR
                     LGLIHYGLLKGLEKSMSFLGGEDFPYGHEGANLLILGQPSLNQLSALRRNIQDRAARE
                     KVNLWLQGSRGLPIFPSFPDHGSPEMAMAMGLDALIIYPNAFSPYLENLAQKWGIPVI
                     LVREDKSFEAIGRQAIGMALDHRKEHAPLSPSRIPFLDSLPRNSILNRVKEVQAALGT
                     GRMKGILLIWGEPNVKQTFFEKTLTLMETALNQKLLVLVGGEAVAQANPLNEELARRM
                     GKTTADISGIQSGNPSYLLSWGEIPRLVHFLRTLGSDREFRQMPVAVSFPELARASTW
                     AAAVSFLSLGLAVQLGTQLPFWGSPSLSEVLLRDWPKISGGSLLASPSLADGQTQARE
                     IISHIQSRSFEK"
     gene            8753..10054
                     /locus_tag="VEL68_09050"
     CDS             8753..10054
                     /locus_tag="VEL68_09050"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ36152.1"
                     /translation="MIGRRKKSPPDRGKQYLIIGNSAAGISAAREIRRRDSRGRITIL
                     SDEPTFGYSRVMLPLYIAGKIGKKEMIIAPGADYSQHRIKLLRGDPVESIHPKEQKVF
                     TRKGMHLPYDQLLVATGSSPRTLDIPGKDLPGIHYLRKINDAEGIRKDLSSSPGPVLV
                     IGGGLVSVKSLEALIHKKRKVHMAISSNRILSQMLDETASGFFLDALQKKGVSVHLQT
                     DVLAFEGKERIEGARLSDGTFLPCSLAIIGKGVKPNTGLLEGTGIQLNEGVVVDPHMA
                     TNLPFIYAAGDVAETLDLLRKEYQGQAIWPLAVEGGRVAGSNMASVASIFTGGLRMNA
                     VELLGIKAVSVGAEEGQNVLTYFPPHKSVYRKLSLSGGRLTGFLLAGDIRCAGILTSL
                     VRNGTPVSTSALEEGLELGFSYSPRFRSLRGEVISGIFGGF"
     gene            10114..10857
                     /locus_tag="VEL68_09055"
     CDS             10114..10857
                     /locus_tag="VEL68_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ36153.1"
                     /translation="MRIDPEKCIGCGKCKSYCTMWGGVMRFERHPKNPARVITVIDED
                     ECVECSVCLRSGCCPTDALFQPALDWPRIVRKNFSDPLKIHPETRIPGRGTEEMKTNE
                     VTGRYRWGKYGMAFELGRPGVGARFYDVEKVAQALAALKVEFEENNPVTKLMVDRKKG
                     LIRPEVLGEKVLSAIVEFMTPAENLPQIFEVLDRVSKEIDTVFSGDIISRVGPEGSVL
                     SVKSLQQMKRFISINGKSNVGLGRPRAAE"
BASE COUNT         2459 a         2980 c         3039 g         2574 t
ORIGIN      
        1 ccggttcagg tttgggaaag agttcataaa cagactgcta ggcgagatcg tgtttgatca
       61 gcttgtcgcc tcggaaacca cacgatcttc ggcacaccgg tgggaagagg gggcgttgcc
      121 tagagaccta agccacattt cctggctcgg agggcaaatc aatatttttt agcctccaag
      181 caacgtgccc cccttccctc atgacttggt tccgcctgcg tggcggcccg cagctgttta
      241 aaacccgtaa agtcgcgctg gattgtccac caggatcttc ttgcgaactg ccggatcggg
      301 cgcccacacg ggcagcaggt tcagcacaag tccgtcatca acctgataca agggggtgac
      361 ttccgtcggc ttgcgtcccg cgggcggcat cgaattggga tggggccagt tggttcccca
      421 caggatgcga tcggggttcg cggcaatcaa cgccttggcg aggggaatga agccggaata
      481 gtcatggcgc ggcccttccg ggatcgatat cttgacgtag gccttcccgg cccggaccag
      541 gtccagaata ccggcaaagc cgggctgttc cacgctcttc tccggctcgg cgccggcgaa
      601 gtgatcgaag acgagcggca cgggtgaagc ggtaagctgg gccttgatcg cctggatcat
      661 ctccatgcta gcaaacagct gtatatgcca gttgcgtttt ttcatgcgct cagctgaggc
      721 ctggaacatt tggacggcga cggccgggtc ggttattccg accgtcttga gattgagccg
      781 gataccgcgg atgccggctt ggcccagggc gtcaagctcg ctatcgggcg tcttgtcgct
      841 gatcacggcg acgccgcgcc cgtcgttccc ccgcgccttt atgccccata gcgtagccga
      901 gttatcgacg ccataaacgc tcggcgtgac gatcaccacc cgctggatgt gaagcgcctt
      961 gtggagcctc gccatctctt ccggcagggc catttccgga gtatagaccc gcccggcgaa
     1021 aaagggaaat tgtgcagggt cgccatggat atgggtatgg caatcgcagg cccccggcgg
     1081 cacctcgaag ttcacttgtg tcattggctg ggatggctta gcaaacaccg tctgggttct
     1141 caggatcact ccagcggcaa ccgctccgac cacaaactca cgccgtgtca acataggttt
     1201 cctcctttcg ttgggtccgt actcggtccc ttacttagct attcgccgaa cgccaaaagc
     1261 ttccccccgg ccctagcttc agtcatcatc gattcttaca gctcatgact ggcggtccag
     1321 aagatgatga tagctgcatt tgcgggaggg tagagctttt tgaaaacagg cgcccgattc
     1381 cagttttggg tggcccggaa aggcccgggt atggtccaac ttgcctctaa gggaattgca
     1441 tgggattagc ctgtgacatt gccctgcctc cttcgttggc acaaggtgag attatcagct
     1501 ttttttcaaa gatgtcaagt cattagaatc ttgggtctgt aaaccagaca tcagaattca
     1561 gggaagaccc actattttcc tcgaatccct ggtcgttttc ccggccgcat gaagtctgag
     1621 gcctaaggat tggggaatga attttacgat ttctgttatt tcattcgttc agtttgatat
     1681 ccgaggtctg gggtccaggg tctgacttcc aaagccagaa gactgttctt gatctgtccg
     1741 ccatcatttg gtatagagca taaaaacgat ggagggaagg ggaatgaaaa aggaaatcat
     1801 cgtttcgcaa aaattgagca agcccctggg gatttattct caggcggtga aagcggaggg
     1861 cagccggctg ctttttattt ccgggatgac ggccagagac aaagacggga acattgtggg
     1921 caagggggat atgaaagccc agacccgcca ggtgttggag aacatcaaag ccgtactgga
     1981 aagggtcggg gtgacctttg atcagattgt taaggtaacg gtctatgtca ccgacatgag
     2041 ccgcttcaag gagatccacg aggtacgggc ccagtatttc aagaaggact atccagccag
     2101 caccctggtc caggtgagcg gtttggtatc cccggaaatg cttattgaga tcgaagcggt
     2161 tgcggttctg gattaatggg aatttgctca cttccgtgag cagaattctc tgccatattc
     2221 ggcctgatga ttgaaaatat gaaaaaccgc taactgataa gggccagctc ctggctggcc
     2281 ttcttcaggt acccttgggc tccgcactgc tggaaaatct caatggctcg gaataggtct
     2341 tcccgggacc tagccgggtg ccccatccgg ttccatatgc gtgcagaaag aagataatcc
     2401 cttcctagaa gaagttgcat cccgttttgt cggtccagtc cgacggcctt gtcggcccac
     2461 tcccgcgcct ggcagaggaa ttgctcgctg aggtggaaca aaatctcggc cgtgtactgg
     2521 gcgatccacc cgctaaaagc cctattcttg ttctggctga aagacttcag gacctcatcc
     2581 aggctgatct ccgaacctcc ctgcaggact ctcgtccggg cgatggccac ccggatcagg
     2641 gtcaaccagg aaggcccgaa ccttttcttc tctaggaagg aaaaggcttt ctggtacgat
     2701 tcaacggatt tttcatacct tcccaggtcg aaatacatgt cgcccaaaaa ggccgaggcc
     2761 caggagcccc aggtgaaatg ggaggtgttc tcgcaaaagc cgatccctcg cagcagataa
     2821 acttcggcta cttcaaataa cccccggtag tagcaggcgg tcccgtagca ggaatgggcc
     2881 attcctttga tgtaaatatc cccgcacgcc tcgccaatgt ccagagcctc ccggctgacc
     2941 tgatgggcct gctccacctt tccctggaaa aggtaattga cgccggtgtt gatcttcgcg
     3001 gaggaaatgc ccatggagct ccgggccgcc atgctcaggt ctagggattt ctggaagtag
     3061 tccaggctct tctggaagtc acaattccaa gacagggcgg tccccataat gtagcatgcg
     3121 ttccagaggg agaaaaaatc tttgttctcc tcggagaatt ccaaagcctg gaccatgtac
     3181 tccatcccct ggacgtaatc ttcctcgaca aaaagcaggt aggacccgat ggcggtatag
     3241 atggacggaa gcccccggcg gtagttgatg cgcagggcca ggtcggcaat cggagagacg
     3301 acttcatagg cttctacgtg gtcgttaagg ttcatgcagt agttcgccag agccacccgg
     3361 gcatccagga ttctctgctg gaccgccggg gtattgggca tcttttccag gcagaaaagt
     3421 tccttgtagg tgtaggagat ggcatcgcca taggccgacc tcttccgcgc cttttttcca
     3481 gccagcttgg cgaactccgc ccctttttcg aaattctcgc tcttgatgaa atgctccgcc
     3541 agaacatcgg tgtgctcgtc gaggttgttc cggtacagct gctccatcac ttggccaatc
     3601 ctttcatgga gctgcttctt cctccggctc agaagagaat catagaccac ctcccgggtc
     3661 agggcgtgtt tgaagacata ggtggaccgg ggataaatat ctctttcgta gagaagctcc
     3721 gcattcttca gaacggaaag gcgggagagg agttcgggct ctgggaggtc ggaagcccgt
     3781 ttgatcaact catggctgaa ttcgcgatcg atgaccgacg cagtctggaa aaggtcccgg
     3841 gctccttccg gaagcgaatc cacccgggcc atgataacct cctgaatggt ggagggaaca
     3901 gtcaaagcgt cgatgttgcg ggccagctgg taatggtggt tcctccggac catgatcttc
     3961 aattcccgaa gagagcggag aaactcctcg atgaatagag ggaccccttc ggtcttctcc
     4021 aggatcagct cctccaggtc ccgactcaga acccgggttc ccaggagatg ggcaaccatg
     4081 gccagggtct cccggttgga gagtcggttc agggtaatct ggctgtggta ggatttggcg
     4141 ttccagctgg gaataaactc cggccggtag gtgaagaagg ccaggattct tgcccccggg
     4201 atcatctcca ggaaggaaag gagggtttct tccgagcttt tgtccatcca gtggaggtct
     4261 tcaaagatca ggatcagggc ccgtgcggcg gccattttca ggaggatccg tttaaaagcc
     4321 tccatgatcc ggtctttcct ggcgtcgggg cttataagaa tcttctcgat cccgctgtct
     4381 ttcaccgaga ggagttccag gagaaaaggg aggtagaggc tttcttccat ctccaggttt
     4441 ttctggcctt cgatgacctt tcttttaatc tccgactctt cgtccccttc ccggacgtcg
     4501 aaagtcgctt tgaggacctc aatgaaggga tggtaggcca tggcccgccc gtaggacaga
     4561 catcttcctt ccaggatcac tccctcttct tcggccactg cctttcgaaa ttcgtagagg
     4621 agccgggatt tccccagccc cgcctccgcc acgatggaaa tgacctgccc ccgcccttct
     4681 ttggctctgg aaaagccatc cttcagcagc tcgatctccc gctcccttcc tacgaaagaa
     4741 gtcaaccctt ttcctgcact cacctcgaac cgtgttttgc tggagctggg ggcgatgacc
     4801 cggtaggttt taaccggctt ttccttccct ttcaccttct tctcccctgc aaactcgaac
     4861 cagaagaggc cttcggtgag ccggtaggtt tcttcactta ccaggatggt ccctgggtcg
     4921 gccatccctt ccatccgaga agccagattc accgtgtctc ccacggcgct gaattccact
     4981 cggagatcac tccccaagct tcccacaacg accgggccgg tgtggattcc gattcgcatt
     5041 tggatgggtg gaatccccct ttcctgcttg cggcgctcgg taaatttact cagctcccgc
     5101 gagatggcca gggcagagcg taaggccctt tgaggggcgt cctccagggc gatgggggcc
     5161 ccgaacaaag ccataatccc atccccggtc atcttgttca cggttccttc aaaggcatga
     5221 acctggtgga tgaggacctc aaagacctgg tccatgagag agtacacttc ctcatggcca
     5281 agcatttctg agagagcggt gaatcccacg atgtcgcaga acaggacggt tacctgcttc
     5341 cgttccccct cgatccgatt tttctgcgcc aggatctttt cggttagccc ttggggaagg
     5401 tacttctgaa tcttcgccaa tttttcattc cagctaaggt cctcagggtg ggtttctttc
     5461 gaatatctcg tgaccggtga agaaagcaga tggccgcatt ccccgcaaaa tttatcatcc
     5521 agctggtttt cagaaccgca ttgaggacat cgggcatgga gctttccccc acattcccga
     5581 caaaacctgc gattcctctg attctcgcta tgacatcggg ggcattttac ctgattgggt
     5641 ttcataaccc cttcaaccct ttacactatt taatcgaaac ggtcttcggc ccctgatcgg
     5701 caaagagagc aaatccggtg ccaagccgaa ttggctcttt aaccccttta aatggttgaa
     5761 aattccagat ccctaggaga aagttcgccc tcttacggga aagaaatttt ccctttaatc
     5821 ctcaaatttt tcattatcca ggtctttacc cgtatctttt tcatcatgca ggaaggtaaa
     5881 ggatcaggac ccttcaggcg gccgtattag acctttttgc gatcccgttg aaaaatcgaa
     5941 tgaaaaaatt gacaggagga tggggttcga ttaacattga ataaaatttc cctatcccgc
     6001 atccctctcc ccctgaacgg ggcgaggggg gcggggcatt ccaatgggag aggggagtct
     6061 tgcgaacatg agaaggtttg tgattgaagt gggaatggga gtggaccagc acggacagga
     6121 taacacccgc gcagcagaaa aggccgttca ggacgccatt caccgaagct gtttgtgcgg
     6181 attgcgggag gttgcgggga tcaagagctc agatgaaatg atggtggaag tcttggtcgg
     6241 ctgccctgct ccagagacag tggacaaaga gagagtcctg aaggttcttc ctctcggtca
     6301 gaaaaggatt gaagtccgaa aaggaggact ctccggtaaa actctctttc aacccgagct
     6361 gggagacaag acggatgaga tggtcgtggc caacgccatc atcacggtgt atgtcccctg
     6421 attcaaatgc ggaattcaga atgtggaatg cggattgccg attgcggatt gaaatacgaa
     6481 gaaatgatgt ccggcttccg gcttattgac ttttgtcatc taagacttga ggcacttgag
     6541 cgcacttaag gcatttgagg cacttttatg aaatccatct tcgtcaatcc ggagcgctgc
     6601 acggcctgcc gaacctgcga aatcgcctgt gcggtgcacc gttcatctct ctcccgaaga
     6661 atgcccgaag cgatctatga ggctgttccc cctttgcccc gagttcgggt cgaaggcact
     6721 ggaacggaga aaggctttcc catccagtgc cggcactgcg aagatgctcc ctgcctggat
     6781 gcctgcccgg ccggtgccct cttccgggac gaaggaggcc tggtcctagt ccacgatgaa
     6841 cgctgcatcg gctgctggat gtgcattatg gtctgccctt tcggcgcacc tcagcccttc
     6901 cggcattttc gcaaggttct aaagtgcgac caatgtaaag gaatggatac ggcctattgc
     6961 gtggaaagct gccccaccca tgccctgatg ctgatcgatc cggaagatat cgccaggggg
     7021 aaattcaagc ctcccaggaa aggaagtctg gtcggattga atttcgcagc gcggtcggcc
     7081 cttttcaaac aggataaatg ataccgttgc ctcacctcgc tttcgcggaa ccagtgaaag
     7141 ggcgaggatg aggggtccat catcttgaat gatttcaaat gattggagaa tgaatgaacc
     7201 ggacccataa aaagagcgcg ctcgcccaga cgtcctctct tatcttggcg cttcatgatc
     7261 agggagagga tctctcctgg gggcgctacg aagagcagct tcccctgtgc gcctttacgt
     7321 ccaatggatt aaactgccgc aagtgctttc aagggccttg ccggatcaat ccctttgggg
     7381 accagcccac ccgaggggtc tgcggtgcgg accgggaaca gatcgtcatg gaaaatctct
     7441 tccaggccac cctggaaggc gtcctggaaa ccgcccggtc cgtctcttct atagatgaat
     7501 ctcttggagg acaggaaatc cctgacttgg attccgatct cccacaggaa accaggaaaa
     7561 gacttgcgga acacgaactc ctccctgttc gaaaaaccca ccttctggaa gttcagaaca
     7621 gttatttctc ccacaagggt tacctctctc gaactctcag ggacctgact cgactgggct
     7681 tgatccacta tggacttctc aaagggctgg aaaaatcaat gtcttttttg ggtggggagg
     7741 atttccctta tggccatgaa ggagccaacc tattaatctt ggggcagcct tccttgaatc
     7801 aactttccgc tctgcgcagg aatatccagg atagggctgc aagggaaaag gttaatctat
     7861 ggctgcaagg aagccgcggt cttcccatct tcccgtcctt tcccgatcac ggctcgcccg
     7921 aaatggctat ggccatgggc cttgatgctc tgatcattta tcccaacgct ttttcccctt
     7981 atctggagaa tttggcgcag aaatggggga ttcccgtcat tctcgtgagg gaagataaat
     8041 cgttcgaggc gattggcagg caagccatcg ggatggctct tgatcaccgg aaggagcatg
     8101 cccctctatc cccttcccgc attccattcc tggactcact cccgcggaat tcgatcttaa
     8161 atcgggtaaa ggaggttcag gcagctctgg gcactggccg aatgaagggt atacttctca
     8221 tctggggaga acccaatgta aagcagactt tcttcgagaa gactctcacc ctgatggaaa
     8281 ccgccttgaa tcagaaattg ctggtcttgg ttggcggaga ggcggttgcc caggcgaacc
     8341 ccttgaacga agaattggcg cggaggatgg ggaagaccac ggcggacatc tcggggattc
     8401 agtccggcaa cccaagttat ctcctatcct ggggagagat tcccaggctg gtccatttcc
     8461 tgaggactct gggttcggat agggaatttc gccaaatgcc tgtggcggtt tcttttcctg
     8521 aacttgcaag ggcctccacc tgggccgctg cagtgagttt tttgtccctg ggccttgccg
     8581 tgcagttggg gacccagctt cccttctggg gaagcccgtc cctttccgaa gtcctgttaa
     8641 gagattggcc aaagatatcc ggaggatctc tactggcctc ccccagcctc gcagacgggc
     8701 aaacacaggc tcgggagata atttctcaca ttcaatctcg aagctttgag aaatgatcgg
     8761 ccgaaggaaa aaatcgcccc cggatcgagg aaagcaatac ctcatcatcg gaaacagcgc
     8821 ggccgggatt tccgcggcca gagaaatcag acgcagggac tcgcggggaa ggatcactat
     8881 tctctccgat gagccgactt tcggttattc ccgggtgatg ctccctctct acattgccgg
     8941 gaaaatcggc aaaaaggaga tgattatcgc cccgggggct gattattctc agcatagaat
     9001 caagctgctg cgcggcgacc cggttgaatc catccacccc aaggagcaaa aagtttttac
     9061 ccgaaaaggg atgcatctcc cctacgacca gctgcttgtg gccaccggat catccccgcg
     9121 caccttggat attccgggga aggacctccc gggcatccac tatcttcgca aaatcaacga
     9181 tgcggagggg atccgcaaag acctttcttc ttctcctggc cctgtactgg taatcggggg
     9241 aggactggtc agcgtaaaga gcctggaggc tttaatccat aaaaagagga aggtccacat
     9301 ggcgatctcc tcaaatcgaa tcctgtccca gatgctggat gaaaccgcct ccggcttttt
     9361 tctggacgct ttgcaaaaga agggagtttc cgtccacctc caaaccgatg tcctcgcgtt
     9421 tgaaggaaaa gaaaggattg agggagcccg cctctctgac gggactttcc tcccttgctc
     9481 gctggctatt atcggcaaag gggtgaaacc caacacaggt ctcctagaag gcaccggaat
     9541 ccaattgaac gagggagtcg tcgtggaccc gcacatggcc accaatctcc ctttcatcta
     9601 cgcagcaggt gatgtggccg agacccttga tctcttgaga aaagaatacc agggccaggc
     9661 catttggccc ctggccgtcg aaggagggcg ggttgccgga tcgaacatgg cctccgtagc
     9721 ttctattttt accgggggcc ttaggatgaa cgcggtcgaa ctcctgggga taaaggccgt
     9781 atcagtaggg gccgaggagg ggcagaatgt cctcacctac tttccgccgc ataaatccgt
     9841 ttaccgtaag ctgtccctct ccggagggcg actcacgggg tttttgctgg ccggagatat
     9901 ccgctgcgcc ggtatcctga cctccctggt gaggaatggg accccggttt caacctctgc
     9961 cttggaggaa ggcctggagc taggtttttc ctattccccg agatttcgtt ctctccgggg
    10021 cgaagttatt tcgggcatat ttgggggttt ctaaacattt caaatttctg gcatattttc
    10081 tttgattctc tgtggtgaaa tacggaggta tttttgcgaa tcgatcctga aaaatgtatc
    10141 ggttgcggca aatgcaaatc ttattgcacg atgtggggcg gggtcatgcg tttcgagcga
    10201 catccgaaaa accccgccag ggtgattacg gtgatcgatg aggatgagtg cgtggaatgc
    10261 agcgtctgcc tgcgcagcgg ctgctgcccc acggacgccc ttttccagcc tgccctggac
    10321 tggccgagga tcgtaagaaa gaattttagc gatcccctga aaatccatcc agaaacccgc
    10381 atccctgggc ggggaaccga ggagatgaaa accaatgaag taaccggtcg gtaccgatgg
    10441 ggaaagtatg gaatggcatt tgagctcgga cggcccggcg taggggcgcg gttttacgat
    10501 gtggaaaaag tcgcccaagc ccttgccgct ctcaaggtgg aattcgagga gaacaacccg
    10561 gtgaccaagc tgatggtgga ccgaaagaaa ggattgattc gtccggaggt cttgggggag
    10621 aaggttcttt cggccatagt ggagttcatg actccggcgg aaaatcttcc ccaaatcttt
    10681 gaagttctcg accgggtatc caaggagatc gacaccgttt tctccgggga catcattagc
    10741 cgggtcggcc ccgaaggctc cgttctttcg gtcaaaagtt tgcagcaaat gaaacggttt
    10801 atctccatca acggcaagag taatgttgga ctggggaggc caagggcggc tgaatgagtt
    10861 tcgggtttct tatttatttt aagcctgagg cacctaagta cacttcagca aacttgaggc
    10921 gcttttcttg aggagctttt catgacccat accctccacc ggcggggcag ccgcgagagt
    10981 ctggactgcg actacatcgt cttcaccatg tcggccaaga gcttcaatga aacagggtcg
    11041 gggaagaaga tg
//
LOCUS       DASPRR010000234         5634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2310001, whole genome shotgun sequence.
ACCESSION   DASPRR010000234 DASPRR010000000
VERSION     DASPRR010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310001"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VEL68_09060"
     CDS             complement(<1..963)
                     /locus_tag="VEL68_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ36154.1"
                     /translation="MLLSPTTFLTIVACSFYGLFVGSIPGLSATLAASLLVPFTFFMD
                     PLPALCSIVSMSAMAIFAGDIPAALIRIPGTPSSAAYTYDSYALTQQGKSQLVLGADI
                     MFSAVGGLMGAAVLMTSAPLLANVAMKFSTFEYFWLAALGLSATVMVSAGSQAKGAIA
                     MCLGLFLSTIGVDITLGYPRFTFGSVDLLNGVSFIPAMIGVFGISEVMRNVSQRRMDF
                     AITKVKTKGIFTGVGHLFRKYQWNMWRSGVIGTIIGVLPGAGADIAAWIAYAASKRAS
                     KEKELYGKGSIEAIVDSGTANNACLAGDWVPALVFGIPGDSITAV"
     gene            complement(1049..1528)
                     /locus_tag="VEL68_09065"
     CDS             complement(1049..1528)
                     /locus_tag="VEL68_09065"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ36155.1"
                     /translation="MGKNEYIGAGIFIALGVFTWAVSFQFPVLPGGHPGPSLFPRVLG
                     TLFIFFSLIVILQGRSKSKTPPPPPAEDEGGNVTLIGKENYFNAVLVIILIAAFIAFA
                     KHLGFIITGGAVLFILMLKLHVRPLAGFIISSIVVCFIYTVFAKGLRVPLPHGFLWW"
     gene            complement(1597..2586)
                     /locus_tag="VEL68_09070"
     CDS             complement(1597..2586)
                     /locus_tag="VEL68_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36156.1"
                     /translation="MRKKTLLRTMMGVSLVAFLILAFAVPGVQAQGKFPDRPMSMICP
                     YGAGGGTDAVARMVAVLMEQDLGQPVNVVNRTGGGGAVGFTAGATAAPDGYTMTLASG
                     DITMLHWMGTAQVSYKEFRAIGQCNFDASAINVRADAPWKTVKELQEYAKANPGKLKA
                     SGTGKGGCWDIARAGWLITAGISPDAIPWVPSSGAAPTLQDMVAGGIPVSTCSLAEAR
                     AMIEAKKVKCLAVMSDKRLNIFPDVPTLKESGVNWSFGVWRGVLVPKGTPDNVVAILE
                     KSLEKAYKNPKYKEFMDKAGLGMVWKSAAEFDKYMAEEDAGNQKVMKASGMIN"
     gene            complement(2913..3974)
                     /locus_tag="VEL68_09075"
     CDS             complement(2913..3974)
                     /locus_tag="VEL68_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HXZ36157.1"
                     /translation="MELKEIYRVAKEKFKGACRVCPLCNGRVCAGEMPGIGGIGTGSS
                     FIANYESLARIKLNLRTIHDVREPDLTYDFFGRKLKMPILVAPVAGMVINMGNAMDER
                     ESLSAMVGGAKEAGSMGCTCDGPNPMFTDLGMEVLRDNDGWGMPTLKPRPEGTFLPLA
                     RRAEECGVNAIATDIDAAGIIHLKKAGQPAGPWPVQDWKKVIFQVKVPVILKGVMTVE
                     DAQGAVEAGAAGIVVSNHGGRVLDHTPGTAEVLPAISSAVKGKIKVFVDGGIRSGADV
                     LKMLALGAEAVLVGRPMAIAAVGGGKEGVTFMLNQYADQLKTAMIYGGCRSLADVSSS
                     VLYSSEKDIRATGKKAKSR"
     gene            complement(4189..5388)
                     /locus_tag="VEL68_09080"
     CDS             complement(4189..5388)
                     /locus_tag="VEL68_09080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36158.1"
                     /translation="MNRQKSQNRFRSLFSFCTYHFIDDGFADSIYLLLPFIAVELHLS
                     FSQVGLLKGVFSGAMSLLQLPMSLLGERIGELTVVSLGAFGLAGGFLLLSWASTFLSV
                     FLTLILAKGTAAGQHGLSSSVLSRVFETSGRRAAMGTYNFSGDVGKVAVPFLLALLIN
                     WLGWRHGIFALSLAGIGAGAVLWALVRRGRMNSAVPQPEPKADSGERGWGIQHRKAFF
                     ALLTIGILDNGTRSSLLTFLPFLLLQKEIPAGQVGFALTLLFAGGAAGKFGCGILAEW
                     FGIIPMVVVTEALTTAGILSLFWVSPSGIWFILPFVGVVLNGTSSVLYATVAEIISPG
                     GRSRGYGLYYAITLGAGAGAPVIYGLVTDWLGLSFSLVCIALMASLTIPLSRYLSKPE
                     PSSPLPT"
BASE COUNT         1312 a         1650 c         1504 g         1168 t
ORIGIN      
        1 gacggccgtg atcgaatccc ccggaatccc gaagacgagc gccgggaccc agtctccggc
       61 caggcaggcg ttgttggccg tgcccgaatc cacaatcgcc tctatggaac cttttccata
      121 gagctccttc tccttggaag ctctcttgga ggcggcatag gctatccagg ccgcaatatc
      181 ggcgccggct cctgggagaa cgccgatgat ggttccgata accccggacc tccacatatt
      241 ccattgatac ttcctaaaga gatgtccgac gcccgtaaaa atgcctttcg tcttgacttt
      301 ggtgatcgca aaatccatcc gcctctggct gacattgcgc atgacttcgg aaatcccgaa
      361 aacgccgatc atggccggaa taaaggaaac cccgttcaga aggtcaacgc ttccgaaagt
      421 gaaacggggg tagccgagcg taatgtccac accaatggtg gagaggaaaa gccccaggca
      481 catggcaatg gcgccctttg cctgcgaacc tgcggacacc atgaccgtag cactgagccc
      541 cagggccgcg agccagaagt actcaaaagt cgagaatttc atggcgacat tggccaagag
      601 cggcgctgag gtcatcagca ccgcggcgcc catcaagccg ccgacagcag aaaacattat
      661 gtccgcgccc agaaccaatt gcgatttccc ttgctgtgtc agggcgtaag aatcgtaggt
      721 gtaggcggca gatgaaggag tccccgggat gcgaatgaga gccgcgggga tgtcgcccgc
      781 aaagatggcc atggccgaca tgctcacgat ggagcacagg gcgggaaggg ggtccatgaa
      841 aaaagtgaac gggaccagca gggaagccgc cagtgtggcg ctcaagccgg gaatcgagcc
      901 gacgaagagg ccgtaaaagg aacaggcgac gattgtcaaa aaggtcgttg gagaaagaag
      961 catgactatt ccgttaacaa aagcatccat agttttcact gtcccttgat cgttctctga
     1021 tcatcacttt gaatcatgaa atccattctc accaccatag aaagccgtgt ggcaggggaa
     1081 ccctcaagcc ctttgcaaac acagtatata tgaagcagac gacaatggac gatatgatga
     1141 agcctgcgag tggcctgaca tgcaatttca acatcagtat gaagagaacc gccccgcctg
     1201 taatgatgaa gcccaaatgc ttggcaaagg caataaaggc agcaataagg ataattacga
     1261 gaaccgcatt gaaataattc tcttttccta ttaatgttac atttcctcct tcatcttcgg
     1321 caggaggagg tggtggcgtt ttggacttac tccggccttg caggatgaca atcagcgaga
     1381 agaagatgaa gagggtgcct aggacacggg ggaagagaga gggcccgggg tgcccgcctg
     1441 gcaggaccgg gaattgaaac gagaccgccc aggtaaaaac acccaaggca atgaaaattc
     1501 cagcgccaat atattcattc tttcccatcg gtgttcctcg agttagggaa agcagtcgga
     1561 accttcccga ccgctttccc atgaaaaagg ttcgggttaa ttaatcattc cggaagcttt
     1621 catgaccttt tggttgcccg cgtcctcctc ggccatgtac ttgtcaaact cggcggccga
     1681 cttccacacc atgcctaaac cggccttgtc catgaattcc ttgtacttgg ggttcttgta
     1741 cgccttctcc agggattttt cgaggatagc aaccacatta tccggggtgc ccttggggac
     1801 caggactcct ctccacacac caaagctcca gttcaccccc gattccttga gagtgggaac
     1861 atctgggaag atgttcagcc gcttgtccga catgaccgcg aggcatttga cctttttggc
     1921 ttcaatcatg gctcgggctt ccgccaggct gcaggtagac accgggatac cgcccgccac
     1981 catatcctga agggtggggg cagcgccgct actggggacc caggggatgg cgtcagggga
     2041 aataccggct gtaatcagcc agcccgcccg ggcgatgtcc cagcatccgc cctttcccgt
     2101 ccccgaggcc ttcagtttgc cgggattcgc cttggcatat tcctgaagct ccttcacggt
     2161 tttccatggt gcgtcggccc ggacattgat cgccgatgca tcgaaattgc actgcccaat
     2221 ggccctaaat tccttatagc ttacctgtgc tgtccccatc cagtggagca tggtaatatc
     2281 ccctgaggcg agggtcatgg tgtacccatc gggagcggcg gtcgcgcccg cggtgaaccc
     2341 gacggctccg cctccgcccg tccggttcac cacattgacc ggctgcccca ggtcttgctc
     2401 catcaggacc gcaaccattc tggccacggc gtccgttccc cctccggcgc cgtaggggca
     2461 aatcatgctc attgggcggt cgggaaactt tccctgcgcc tggacccccg gaacagcgaa
     2521 ggccaggatc aaaaaggcca ccaaactgac acccatcatc gtccgaagta acgttttctt
     2581 cctcatgatc tttcctctcc tttcatttta gggttaatgg ttcaaagaga tttccttcca
     2641 ttgagacgcg ggaagtgcat ccagcctcaa tttcaacgaa atacaactag ccatcatcag
     2701 ggcagcagga cggtccttca atgaatcccg tgccgagaat cttcgccagc ttttccactt
     2761 caccaagtct gattttcata gacacctccc attggtttac tggccgaata aactccctcc
     2821 ctgtcatcaa aggttaagaa gcgccgtctg cgcgccgttt gaaccccttt gaaagtggcc
     2881 ggaaaaggac aaggccgaat ttcatgaaaa tctcaacggg atttcgcttt cttcccagtg
     2941 gccctgatat ctttttcgga ggaatacaag accgaagagg aaacatcggc cagcgacctg
     3001 catccgccat aaatcatggc cgtcttcagc tggtcggcat actggttgag catgaaggtt
     3061 acgccctctt ttcctccccc caccgcggcg atggccatgg gccgacccac caggacagct
     3121 tcggctccca gggccagcat cttcagtaca tccgccccgc tgcggatccc gccgtcgacg
     3181 aagaccttga ttttgccctt caccgccgag gaaatggctg gaagcacttc cgccgtcccc
     3241 ggggtgtgat cgagaactct gcccccgtgg ttggacacta cgatacctgc agcccccgcc
     3301 tctacggccc cctgggcatc ttcaaccgtc atgactcctt tgagaatcac tgggaccttc
     3361 acctggaaga taaccttttt ccagtcttgc accggccagg gtccggcggg ctgtcccgcc
     3421 ttcttcaaat gaataatgcc tgcggcatcg atgtctgtgg cgatggcatt gacgccgcat
     3481 tcttccgccc tccgggccag ggggaggaaa gttccctccg gccgcggttt cagcgtgggc
     3541 atcccccagc cgtcgttatc ccgcaggacc tccattccca ggtcggtaaa catgggattc
     3601 gggccgtcgc aggtgcagcc catggaccct gcctctttcg cacccccaac catggccgaa
     3661 agggactccc gctcgtccat ggcgtttccc atgttgatga ccattccagc caccggcgcc
     3721 acgaggatag gcattttcag tttccgcccg aagaaatcat aggtgagatc cggttcccgg
     3781 acatcgtgaa tggtccgcag gttcaacttg atccgggcca ggctttcata gttggcgatg
     3841 aaagaggagc cggttccaat ccccccgatt ccgggcatct ctcccgcgca gacccttccg
     3901 ttgcacaacg ggcagacccg gcaggcccct ttgaactttt ccttggcaac tcggtaaatc
     3961 tcttttaatt ccatggctag tttccttcct ttaaagtttg caccccagcc gctgtaaaac
     4021 tttcccccct gacggcttcg attcggccgc cgttcttcag gatggacatt tgcgggactt
     4081 tccagaggat ggcttactgg cagatagaag aaggataatc ccactccatc atcgcttttt
     4141 ctattgtatc ttgaatttct caaaaaacaa ggctctgccg gcccccactc aggtggggag
     4201 tggagaggag ggttcgggct tggagagata gcggctcagg ggaatggtta aggaagccat
     4261 cagggcgatg cataccaggc tgaatgagag tccgagccaa tcggtcacca aaccgtagat
     4321 caccggcgcc ccggctccag cccccagggt gatggcatag taaagcccat acccccggga
     4381 gcgtccacct ggggatatga tctccgctac tgttgcataa agcacggaag aagttccgtt
     4441 caacacgacc cccacaaagg gcaggataaa ccagatgccg gatggggaca cccagaagag
     4501 agataagatc ccggcggtgg ttaaagcctc ggtaaccacc accatgggga tgatcccgaa
     4561 ccattcggcc aggattccgc agccgaattt ccccgccgct cccccggcga aaagcagggt
     4621 cagggcgaaa cccacttggc ccgccgggat ttctttctgc aggagaagga agggcaaaaa
     4681 ggtgagtagg gatgaccggg tgccattatc caaaatcccg atggtaagga gtgcgaaaaa
     4741 ggcttttcga tgttggatcc cccatcccct ttccccgctg tctgccttcg gttcgggctg
     4801 gggtacagcg gaattcattc tcccccttcg gaccagagcc cagaggaccg ctccagcgcc
     4861 gatgccggca agagaaaggg caaagatccc gtgcctccag cccagccagt ttatgagcag
     4921 ggccagaagg aaggggacgg ccactttccc cacgtctccc gagaaattgt aggttcccat
     4981 ggctgcccgg cgcccggagg tttcaaaaac ccgggagagc acggacgagc tcaggccgtg
     5041 ctggcctgct gcagtccctt tggccaggat gagggtcaga aaaaccgaaa ggaaggtgga
     5101 ggcccaactc aggagcagaa aacctccggc taggccaaaa gctccaaggg aaaccaccgt
     5161 gagctcccca attctttccc ctagaaggct catgggaagc tggagcaggc tcatggctcc
     5221 agaaaaaacc cctttcagca gcccgacctg actgaaggag agatggagtt caacggcgat
     5281 gaaggggaga aggagataga tggaatcggc gaatccatcg tcgataaagt gataggtgca
     5341 gaatgagaaa agggagcgga agcggttttg gcttttttgg cgattcaaag gttcggctta
     5401 gccttcagcg tttgatcctc ggaccttcat gatccttgct ccgttcactc cgttgcctaa
     5461 ctcatctttt cctaaagaaa ggctttctcc aagagggtaa tgtctctccg ccggtaagcc
     5521 gagacgacct ccgcgcagaa taaaagaatg atcgatgaat aaaacaccca gattaccagg
     5581 atgacgatgg cctccagcga tccgtagacc acgttatact gggttgaccg tcct
//
LOCUS       DASPRR010000235         7194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1830260, whole genome shotgun sequence.
ACCESSION   DASPRR010000235 DASPRR010000000
VERSION     DASPRR010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830260"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..903)
                     /locus_tag="VEL68_09085"
     CDS             complement(46..903)
                     /locus_tag="VEL68_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36159.1"
                     /translation="MSLFIQQVLNGIMLGSVYSLVALGLTLEYGILHIPNFTHGALYM
                     AGAYVTFLLATSAGLNFWLAMVISMIALALIGIVMERLVYRPLMLESPLSSFIAAIGM
                     IFIFVDGALYLFGPDFKRFPPVHTQVVQVLGTTITFQRIIIILVTVTLIVLLHLFIKK
                     TIVGATIEATAQDMEGARLMGINVKRVRMMVFALGTALAAAAASLIAPIHLVHPGMGG
                     PVILKAFVIIILGGMGSIPGAIIGGFLLGLVESLSGGYISTSYHEVFSFGVLVTVLAI
                     RPTGLFGGK"
     gene            complement(1078..2283)
                     /locus_tag="VEL68_09090"
     CDS             complement(1078..2283)
                     /locus_tag="VEL68_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36160.1"
                     /translation="MDKGRFFSFSIPVTGIISFLLMTWVAPSGAAEVINVGYTGPLSG
                     GAAKYGQNNLDGLKMAVDEINEAGGVSVAGKTYTFKVNSLDDRYRPADAVTNARRFVE
                     LQKPPFIFCPHSGGILGMLKFNEQEGFILHGYTDNTAILQQGNKWLVKAPMSMGFYNY
                     GVMEIGWKKGWRKGALLCGTHEAAKMGEKVFEVLWKDMGGEIVANAPADYQKVTDFYP
                     YLTKVLAAKPDAIFLYGPSEPSAMIISSARELGFKGGFMLGSQCKLDEMEKVAPLKTL
                     TPSVGVCPVGMMPLPLMQEYSKRHEQRVKTIPTSESAFNYEVMYIFAEAMKKAATVKD
                     LDQIMAAIPGVLPVGKHAIRGIHSMTKEGQFISGYFAMEIADGKFASPANIDPGPWYK
                     KYGAAWTPK"
     gene            complement(2589..3182)
                     /locus_tag="VEL68_09095"
     CDS             complement(2589..3182)
                     /locus_tag="VEL68_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36161.1"
                     /translation="MKHRRQEVIWLIMLLTLFASPSCSLKYSPRSAEEFRQETLRLEE
                     KARSHEDPEVRAECQLELAYLYLHYRNPLLNYGKALQGFEAYLRFTAADRQTDEILNW
                     TLALREMDGREREKASLKERVAKLAQENEGKTKSLAWQLKKNQETQSALEKIQAQVDS
                     LERTNRSLKEANDKMKETVEKLKNLDLQMEEKRKAIK"
     gene            complement(3211..4572)
                     /locus_tag="VEL68_09100"
     CDS             complement(3211..4572)
                     /locus_tag="VEL68_09100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXZ36162.1"
                     /translation="MKETILKFIRLSIGKKLLLGFLSYGIITLLIALFALSSLDQLNM
                     INSSIIQRDIPLMEICDKMVENLLAQELYAGRSIILQSAEVTSLFWKRNEEFKRLILQ
                     LKTLPQPESVPIESLTALHEEYSWFLKEGFKASESSATKAKEFERQAKEKQEALIQVV
                     RKISWAAKQDQMEKNQKIAEIGNRAFRVTAGLSVVGILLGILVTLIITRNISGPIHQL
                     KLSTREISEGRFDDLPMVQSGDELEDLSKAFKEMAGRLKKLEEMYLDANPLTRLPGGV
                     AIEEVLKVKLAEEAPLAFCLFDLRHFKGFNDRYGYARGNEVILATAEIIREAVREHGG
                     EGDFVGHIGGDDFVLITGPGRYESICKAVIEAFDRKIQAFYDLEDRQRGYIRGTTRTG
                     QVVSFPIMILSIAVVTNQQRMLKSHIQVGEIAAEMKNYAKTFSKSTYVVDRRRDRPPE
                     GTA"
     gene            5071..6228
                     /gene="acd"
                     /locus_tag="VEL68_09105"
     CDS             5071..6228
                     /gene="acd"
                     /locus_tag="VEL68_09105"
                     /EC_number="1.3.99.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaryl-CoA dehydrogenase Acd"
                     /protein_id="HXZ36163.1"
                     /translation="MSFEFSDEVRAIKDMAAKFAQKEILPTVAQSEKKHEFQRDLVKK
                     MGELGFYGCPLPEEYGGSGLGFLAHAVVTEEISKVSGALRAPFNMQTMGTAREIYQYG
                     NEAQKKKYIPRILSGEFLGCIGITEPNAGSDVGAIKTKAVKKGDFYVLTGSKNWITYA
                     QVADVGIIYAYTNPAVKYKGLTAFIVEMKWPGIKTGPTAEKLGWNACPTGEIFFEEVQ
                     VPAENLLGEEGRGFEYVMGSLDNTRLTAAAGAVGVSQGLIDEAVNYAKAREQFGKPVG
                     QYQMVQEELARMIVETEAARLLTYRCGWQKDKGVRNTLETSMAKYYSCDVASRVADGA
                     LRILGAYGYSAEYPVERLFRDAKLYQILEGSANIHKMIIANDALGYRKANR"
     gene            6281..7012
                     /locus_tag="VEL68_09110"
     CDS             6281..7012
                     /locus_tag="VEL68_09110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HXZ36164.1"
                     /translation="MNELMGKIALITGGSRGIGRSCALALAQAGADVVVNYRQRQDAA
                     EEVCSLVKGYGRRAFHYRADVSNSTEVNRMVKAVEQQMGTVEILVNNAGIARRYSWDQ
                     IPEEGWDEIIDTNLKSAFLVTQAVLPGMRARKWGRIINISSGAAATGGVVGPYYAASK
                     AGMLGLTHGYASLTLKEGITVNAVAPSLIQTDMAQQDLRVDPKSTPIGRLGTPEEVAG
                     VVVMLATTGYITGQTIFVNGGRHMT"
BASE COUNT         1624 a         1994 c         1962 g         1614 t
ORIGIN      
        1 ggatgatcag acgcttgggg gaataaagat tggccggatc ctccatcact ttcccccgaa
       61 tagcccggtg ggccgaatgg ccaggaccgt caccaagacc ccgaaagaga agacttcatg
      121 gtaagaggtg ctgatgtatc ccccgctcag gctctccacc agccccaaaa gaaacccgcc
      181 gatgatcgct ccggggatgc ttcccatccc gcccaggatg atgatgacga aagccttcag
      241 gatgaccggc ccgcccattc ccggatggac cagatgaatc ggagcgatca ggctggcagc
      301 ggctgcggcc agagcagtcc ccagggcaaa aaccatcatc cgaacccgct tcacattgat
      361 gcccatgagg cgggcccctt ccatatcctg ggccgtggct tcgatcgtcg ctcccacaat
      421 ggttttcttg atgaagagat gcaggagcac gatcaaagtc accgtaacca agataatgat
      481 gattcgctgg aaggtaatgg tggttccaag gacctggacg acctgggtgt gcaccggcgg
      541 aaaccgtttg aaatccggtc cgaaaaggta cagcgcccca tccacgaaaa taaagatcat
      601 tccgatggcg gcgataaaag agctcagggg ggattccagc atgaggggac gatagaccag
      661 ccgctccatg acgatgccga tcagagccag ggctatcatg gaaatgacca tggccagcca
      721 gaagttgagg cccgccgacg tggccagaag gaaggtcaca taggcaccgg ccatgtacag
      781 tgccccatgg gtgaagttag gaatatgcag gatcccgtac tccagggtca gcccgagggc
      841 caccagggaa tacacgctcc ccagcatgat cccgttcagc acctgctgga tgaaaagaga
      901 catctaaact ccctaaaatg aaaaacgcat agcgcatagg catagcgtat cgcattaaga
      961 ttccactggc tcatggctga tagaaaagat tctctcccgc aggctatgaa ccgtgagcca
     1021 tccgctcttt gccctgcctt ctttgctatg ctctatgcgc tatgttcttt gcggttttta
     1081 tttgggggtc caggccgccc cgtatttctt gtaccacggg cccgggtcga tgttggccgg
     1141 cgaggcgaat ttcccgtcgg cgatctccat ggcgaaatag ccggagatga actgcccttc
     1201 cttggtcatg ctgtgaatcc cgcgaatggc gtgttttccc accggcagga cccccggaat
     1261 ggcggccatg atctggtcca ggtccttgac ggttgcggcc ttcttcatgg cctcggcgaa
     1321 gatgtacatc acctcgtagt tgaaggccga ttccgaggtg ggaatggttt tcaccctttg
     1381 ctcatgcctc ttcgaatact cctgcatgag cggaaggggc atcatgccca cgggacaaac
     1441 ccccacactg ggagtcagag tctttaaagg agccaccttc tccatctcgt ccagcttgca
     1501 ttgggaaccc agcatgaatc cacccttgaa accaagctcg cgggcactgg agatgatcat
     1561 ggcggacggt tctgacgggc cgtagagaaa gatggcatcg ggctttgccg ccaggacctt
     1621 ggtcaggtag ggatagaaat cggttacctt ctggtaatcc gcaggggcgt tggccacgat
     1681 ttcacccccc atgtccttcc ataatacttc aaagactttc tcccccattt tggccgcctc
     1741 atgggtcccg cagaggaggg ctcctttcct ccatcccttt ttccagccta tttccatgac
     1801 cccataattg taaaatccca tggacatggg ggctttgacc agccatttgt ttccctgctg
     1861 caagatagcc gtattgtccg tgtagccgtg caggatgaaa ccctcctgct cgttgaactt
     1921 cagcatcccc aggattcccc cgctgtgagg gcagaagata aacggcggtt tttgcagttc
     1981 cacgaacctt cgggcgttgg tgaccgcgtc cgccggccgg taccggtcgt cgagggaatt
     2041 tactttaaag gtgtaggtct ttcccgcaac ggagaccccc ccggcttcat tgatctcgtc
     2101 cacggccatc ttgaggccgt ccaggttatt ctgcccgtac ttggccgctc cgccgctcag
     2161 ggggccggtg tagcccacat tgataacctc tgcggccccg gagggtgcga cccaggtcat
     2221 cagcaggaag ctgataatcc cggtcaccgg gatcgagaaa gaaaagaatc ttcccttatc
     2281 catgacaacc tccttcctgg caaaagagta aaaatctcca cctgcccccc aggcatccct
     2341 tccggcgctg aaggaatgct actggcgatc gatgaccttc tcgttctaag gaataatcct
     2401 ggcgaagggc cgggaaaacc aaaaaatcta tttagggact ccggcaggtt ttcctgaaaa
     2461 tccggccctg acggtttcgg tcttctctct cacttctgac cccagagatt tatgcaacgt
     2521 gacatgtttc gtgtgcgggg aattttttct gaccgatctt agcaatccgt ggaaatatgt
     2581 caagggaatt atttgatcgc ttttctcttc tcctccatct ggaggtcgag atttttcagt
     2641 ttctccaccg tctctttcat tttatcattg gcttctttca ggctccggtt cgtccgttcc
     2701 agggaatcca cctgggcctg tattttttcc agggcagact gcgtttcttg attctttttc
     2761 aactgccaag ccagagattt agtctttccc tcgttttcct gggccaattt tgccactctc
     2821 tccttcaagc tggccttttc cctttcccgc ccatccatct cccggagggc caaagtccaa
     2881 ttcagaatct catcggtctg ccggtccgcc gccgtgaacc tcaggtaggc ttcgaatccc
     2941 tggagtgctt ttccgtaatt caaaagtgga ttccggtagt gaagatagag ataggccagc
     3001 tccaactggc attccgcccg gacctcgggg tcttcgtggc ttcgggcctt ttcctccaga
     3061 cggagggtct cctgcctgaa ttcctctgcc gacctgggag aatatttcag gctgcagctg
     3121 ggagaagcaa acagggtgag aagcatgatg agccagatga cttcctgacg tctatgtttc
     3181 ataacctctc cttggggttg ggaggaatta tcaagctgtt ccttccgggg gcctgtcgcg
     3241 ccgcctatcg actacatagg tggacttgga aaaggtcttg gcgtaattct tcatctcggc
     3301 ggcgatttct cccacttgga tgtggctttt cagcatccgt tgttggttgg taaccacggc
     3361 gatggatagg atcatgatgg gaaaggagac gacctgcccg gttcgggtgg tcccccgaat
     3421 atatcccctc tgccggtctt ccagatcata gaaggcttgg attttccggt cgaaggcctc
     3481 gatgaccgcc ttgcagatac tctcatagcg gcccgggccg gtgatcagga caaaatcatc
     3541 cccgccgatg tgaccgacaa agtctccctc ccctccgtgc tcccgaaccg cctcccggat
     3601 gatctccgcc gtggccagga taacctcatt tccccgggcg taaccgtagc gatcattgaa
     3661 gcccttgaag tggcgaaggt caaagaggca gaaagccagg ggggcttcct ctgccaattt
     3721 gaccttcaaa acttcctcga tggccacccc gccgggcaga cgggtgagcg ggttggcatc
     3781 caggtacatc tcctccagct tttttagccg cccggccatt tccttaaagg ccttggacag
     3841 gtcttccagt tcgtccccgc tctggaccat gggaaggtcg tcgaacctcc cttcggaaat
     3901 ttcccttgta gacagcttca gctggtgaat cggccctgag atgttccggg tgatgatcag
     3961 ggttaccaga attccaagga gaattcccac cacgctcagc ccagccgtca cccgaaaagc
     4021 ccgattgccg atttctgcga tcttctgatt tttttccatc tggtcctgct tcgctgccca
     4081 ggagattttc ctcaccacct gaatgagggc ctcctgcttc tctttggcct gacgctcgaa
     4141 ctctttggcc tttgttgcgg aggattccga agccttgaat ccctccttga ggaaccagga
     4201 atactcctca tggagggcgg tcaacgattc gatgggaacg ctctcgggct ggggaagggt
     4261 tttcaactgg agaatcaatc tcttgaactc ctcgttcctc ttccagaaga gggaggtcac
     4321 ctccgcgctc tggaggatga tcgaacggcc ggcgtagagt tcctgagcca gcagattttc
     4381 caccatctta tcgcaaattt ccatgagggg gatgtccctc tggatgatgc tagaattgat
     4441 catgttcaac tgatccaggc tggataaggc aaagagggca atcaggaggg ttataattcc
     4501 ataggagaga aaacccaaga gcaatttttt gccgatgcta agccggatga acttcaggat
     4561 cgtttccttc atgaattcgc caccccgttt ccggtccgaa ggtccccggg aaagtccagc
     4621 actttccagg ctgcccgttg ggtgattata ccctaaaagt tttatcctac ccagtgaaat
     4681 tcacggaaga aaccaaaacg gtctctttgc ttttctccgg cgagagacgc gactcgggcc
     4741 acgagaagcg gggacttaat agtgaaggtg gaaaggatag agattgaatt gacaatccga
     4801 tcaaccgatg gtaatgatgg gggtcagacc caccgggatg ggatgagata acccttcctg
     4861 aacggggatt cataatggag attgcctctt cagcgacgga aaggaacatg agtctttggg
     4921 tcggccattt tactggaacc gcgcatggtc ttcaaatcag gggagatgag cccggcctat
     4981 tcacgctatt aaatccctct ccctgttcac gacttgactc agtgcccctt taagaaattg
     5041 ggcaagaaat ccaaatccgg aggaaaaaac atgagcttcg aattcagcga cgaggtcaga
     5101 gccataaagg atatggccgc gaagtttgcc caaaaagaaa ttctccccac cgtggcccag
     5161 agcgagaaga aacacgagtt ccagcgggac ctggtcaaaa aaatgggaga actgggcttt
     5221 tacggctgcc ccctccccga ggaatacgga ggctccggcc tgggtttcct ggctcacgcc
     5281 gtggtgaccg aggagatttc caaggtcagc ggggcgttgc gcgccccttt caatatgcag
     5341 accatgggaa cggcccggga aatttaccag tacggaaatg aagcccagaa gaaaaaatac
     5401 attccccgga tcctttccgg cgagttcctg gggtgcatcg ggatcaccga gcccaacgcc
     5461 gggtcggacg ttggggcgat caagaccaag gcggttaaga agggagactt ctacgtctta
     5521 accggatcga agaactggat cacttacgcc caggtggccg acgtgggcat catctatgcc
     5581 tacaccaacc ccgcggtcaa gtacaaaggg cttacggcct ttatcgtgga aatgaaatgg
     5641 cccgggatca agaccgggcc cacggcggag aaactggggt ggaacgcttg cccgacgggg
     5701 gaaatattct tcgaagaggt ccaggtccct gccgaaaacc tgctgggcga ggagggacgg
     5761 gggttcgagt atgtcatggg gagcttggat aatacccggc tgaccgctgc cgccggagcg
     5821 gtcggcgtaa gtcaggggct gatcgacgaa gcggtgaatt acgccaaagc cagagagcag
     5881 ttcggcaaac ccgtggggca gtaccagatg gtccaggaag agctcgcccg aatgatcgtg
     5941 gagaccgaag ccgctcggct cctcacctat cgatgcggtt ggcagaagga taaaggggtc
     6001 cggaataccc tcgagacctc catggctaag tattattcct gcgatgtggc ttcgcgggtc
     6061 gccgacgggg cgctcagaat tctgggagcc tatggatatt cggcggagta tccggtagaa
     6121 cggctcttcc gcgatgccaa actctaccag atcctggaag gttcagccaa cattcacaaa
     6181 atgatcattg ccaacgatgc cctgggatat cggaaggcca atcgctaatt cccggaatat
     6241 cagcggtcga gaaacgggga ggcccaaaaa ggggatggac atgaatgaac tgatggggaa
     6301 gattgctttg atcaccggcg gcagccgggg aatcgggcgg tcctgcgccc tggccctggc
     6361 ccaagcggga gccgatgttg tagtaaatta ccggcaacgc caggatgcag ctgaagaggt
     6421 ttgctctctc gtgaaaggct acggaaggcg agcctttcat taccgggcag atgtctccaa
     6481 ctcaaccgaa gtgaaccgca tggttaaagc ggttgaacag cagatgggca cggttgagat
     6541 tctggttaat aacgccggca ttgcccgccg ttattcatgg gaccagattc ccgaagaagg
     6601 ttgggatgag atcattgata ccaacctgaa atccgccttc ttggtaaccc aggccgtact
     6661 tcctggcatg cgcgcccgaa aatggggccg aatcatcaat atttcgtcgg gcgcggccgc
     6721 cactggcggg gtagtcgggc cctattacgc cgccagcaaa gccgggatgt tgggactgac
     6781 ccatggatac gcctccctca ccctaaaaga gggcatcacc gtcaacgcgg ttgctccgtc
     6841 tttgattcag acggacatgg cccagcagga cctccgcgtc gatccaaaat cgacccccat
     6901 cggccgcctg gggaccccgg aagaagttgc cggggtggtg gtcatgctgg ccacgactgg
     6961 ctatataacc ggccaaacga tcttcgtgaa cggcggaagg catatgacct gacaattgcg
     7021 gattgccaat agcattttcg gattgtatga taaacaccca ttgaattcgg taaacagaac
     7081 ccggaattca gcattcaaat aatacctgga gagaattttg gctcctgact catggcttcg
     7141 gcattcccaa tccttctatt ccgcaatccg caatggtgag ggggttgatt tttt
//
LOCUS       DASPRR010000236         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_207717, whole genome shotgun sequence.
ACCESSION   DASPRR010000236 DASPRR010000000
VERSION     DASPRR010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207717"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VEL68_09115"
     CDS             complement(<1..488)
                     /locus_tag="VEL68_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ36165.1"
                     /translation="MVKMKGYMGKILRVDLTKGRAEAETLDENLAKKFIGGTGLATKI
                     LYDETGPETDPLGPKNRLIFMTGPFAATPVITSGRHHMVTKSPLTGAYTESDTGGTWG
                     PFLKRAGFDGVVVTGKARKPVYLWVTEGKAEIRDASRFWGMDTYVLDEAIRKDTHSEA
                     VVA"
     gene            complement(636..902)
                     /locus_tag="VEL68_09120"
     CDS             complement(636..902)
                     /locus_tag="VEL68_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ36166.1"
                     /translation="MKIRFFKPFDDLSGRDEIDLDLKRSVKVKEVLRMIEKEVPSFQR
                     FLQKEKDEVLNFFVVLVRGDEILKLNDTVQDKDVVKVLPPISGG"
     gene            complement(1053..2426)
                     /locus_tag="VEL68_09125"
     CDS             complement(1053..2426)
                     /locus_tag="VEL68_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ36167.1"
                     /translation="MEKPWLKFYDPHVPKALEIPPILLPQVLDEAAGQFPDQVGVFFF
                     GGKVRYRDLKTGADQFARALAEIGVKRGGRLGLLLPNMPQTIISAFGGMKAGVLVFFF
                     DPLLEEDELRRQINESGAETVVVLDLLLRRIDPIFPQTRLKNFVIAGVKDFLPFPRNL
                     LFSLAARGRGIHVKLAQKPNIYPFQEFLQKGRADQPMVDGNPLNPDDPAVVQYTSGKT
                     GPPKGVLLTHRNLVSNILQVSAWFNNLKKGKEVFFSILPCHQALGLTLAVNLPIYLAG
                     SSIHQPRFDPIQFLNTVKAQRPSAFPAQPAMIESLVWNPTLGKYRISSIQTYWSIGPS
                     LAPEVLENYERKTGRRINEGYGLTEAAPLTHMNPFLGDRKPKSMGIPLPDTDARIIDP
                     QDGRAELPIGKTGQLIIRGPQVMKGYWNQSEETERALREGWLHTGDLAWMDEDGFFYF
                     VDRMKKK"
     gene            complement(2534..3484)
                     /locus_tag="VEL68_09130"
     CDS             complement(2534..3484)
                     /locus_tag="VEL68_09130"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXZ36168.1"
                     /translation="MNVLVTGGAGFIGSHLVERLLKDGHGIVCLDNFDDFYDPALKGR
                     NLSRALQNSQFKLIEGDLRDEKLMGQIFEKEKIDGVFHLAARAGVRPSLQNPALYADV
                     NIRGTIYLLEACRQHGVRRFIFASSSSVYGNSSRVPFSEEDPVNHPISPYAATKKAGE
                     LLCHTYHHLYGMDIACLRYFTVYGPRQRPEMAIHHFIRSIFEGRRISLFGDGSSRRDY
                     TYIEDIIEGTLGAFSRENGFQIYNLGESQTISLSELIREIEGQAGKKAVLEYLPEQPG
                     DVRQTYADIRKAREMLGYNPRTKVREGLARFVQWFLEGMK"
     gene            complement(3569..>4119)
                     /gene="rlmD"
                     /locus_tag="VEL68_09135"
     CDS             complement(3569..>4119)
                     /gene="rlmD"
                     /locus_tag="VEL68_09135"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HXZ36169.1"
                     /translation="LLLQAGGKSFVQVNPDQNRNLIGKVLEWADLKREEKGIDLFCGS
                     GNLTLPLALRGGKVWGVDQDIEAIDSARENARRNLIGNCTFWAGAAEEGIGRIKRETG
                     AVDLVVLDPPRVGAHSALIPLASLHPRRILYVSCEPPTLARDLAHLVSLGYNLKRIQP
                     LDMFPQTYHIEVIAELIAGEKM"
BASE COUNT          894 a         1149 c         1075 g         1001 t
ORIGIN      
        1 gccacgacag cctccgagtg ggtatccttg cggatggcct catccagtac ataggtgtcc
       61 attccccaga accgggaggc atcgcggatt tccgcttttc cttcagtcac ccataggtaa
      121 acgggtttgc gggccttgcc cgtgaccacc accccgtcaa agccggctct cttcaggaaa
      181 ggcccccagg ttccgccggt atcagactcg gtataggctc cggtgagggg tgatttggtg
      241 accatgtgat gccgccccga ggtgatgacc ggtgtggccg caaagggtcc ggtcatgaag
      301 atcagccgat ttttcggtcc cagcggatcg gtttctgggc cggtttcatc atagaggatc
      361 ttggtggcca ggccggttcc gccgatgaac ttcttggcca ggttttcgtc cagcgtttcg
      421 gcctccgcgc gacccttggt gagatcaacc cgcagaatct ttcccatgta ccctttcatc
      481 ttcaccatcc tccttttgaa gaattacttc tttttcatcc attcgaatct cttttgaatt
      541 tgctgccggt ccgctttttg aaaaaacgtg ggaacaggta tcagctaaaa aagaaaggct
      601 gacggctgaa acctgattgc cgatcgctgt ttcggttatc ctccactgat cggggggagg
      661 actttgacga cgtccttgtc ctggaccgtg tcattcaatt tcaggatctc gtccccccgg
      721 accaggacga cgaagaaatt caacacttcg tccttctctt tctggagaaa tcgctggaag
      781 gagggaactt ccttctcgat catcctcagg acttctttga ctttcaccga tcttttcaaa
      841 tccagatcga tctcatctct gccggaaaga tcatcaaagg gtttgaagaa gcggattttc
      901 atgaaacctc ttcctttcat ttccccactg tttattttcg cccccgctta aagtggaagt
      961 caagataaaa gaaggtccca gggttcaggc ttcgggtctc aggccactcc atcactcgat
     1021 tttcctgaca cctgaatcct ggtgcctgga acctatttct ttttcatcct atccacaaaa
     1081 tagaagaacc cgtcttcatc catccaggcg aggtcgccgg tatggagcca cccttcgcgc
     1141 agggcccgct ccgtctcttc ggattgattc cagtacccct tcatgacctg aggaccccgg
     1201 atgatgagtt ggccggtttt tccgatgggc aattccgccc tcccgtcttg gggatcgata
     1261 atcctggcat ccgtatccgg cagggggatg cccatggact tcggctttcg atctccaagg
     1321 aacggattca tatgggtcag gggagcggct tcggtgaggc cgtatccttc gttgattctc
     1381 ctcccggtct ttcgttcata attttccagg acctccggcg ccagggacgg tccgatggac
     1441 caataggtct ggatggagga aatcctgtac ttgcccagcg tcggattcca aaccagagac
     1501 tcgatcatgg ccggctgagc aggaaaggcg gaaggccttt gagctttcac tgtattcagg
     1561 aattggatcg gatcgaaacg gggctggtgg atactgctcc cggccagata aatgggcagg
     1621 ttcaccgcca gggtcaatcc caaagcctga tggcagggca ggatggagaa aaaaacctcc
     1681 ttcccctttt ttaggttgtt gaaccatgca gacacctgga gaatattgct gaccagattt
     1741 ctatgggtca agaggactcc tttgggtggg ccggtcttgc cgctcgtata ttggaccacg
     1801 gccggatcgt ctggattcag gggattgcca tctaccatcg gctggtccgc ccgccctttt
     1861 tggaggaact cttggaaggg ataaatattg ggtttctggg ccaatttcac atgaatcccc
     1921 cgaccacggg cggcaaggga gaagaggaga ttccgcggga agggaagaaa atccttcact
     1981 ccggcgatga cgaaattctt caggcgcgtt tgagggaaaa tgggatcgat gcggcgaagc
     2041 agcaaatcca ggaccaccac ggtttccgcc cctgactcat tgatctgccg ccgcaactcg
     2101 tcctcttcta aaaggggatc aaagaaaaat accagaactc cggctttcat tcccccgaaa
     2161 gcgctgatga tggtctgagg catattgggc aggaggagac ccaaacgccc tcctcttttc
     2221 accccgattt ctgccagggc ccgggcgaat tgatcggccc ccgttttcaa atctcgatat
     2281 ctgactttcc ccccgaagaa aaagaccccc acctggtccg gaaattgacc cgccgcttca
     2341 tccaagacct ggggcagaag gatggggggg atttccagcg ctttgggaac atgggggtca
     2401 tagaatttca gccaaggctt ttccatcttc tttccttctc gaatgggacg cagattttcg
     2461 cagacatacg cagattcttt ttggggtttt taaaaatcca taaaatctgc gttcatcttc
     2521 gcccgaaatg tctctatttc attccttcga ggaaccactg aacaaagcgg gccaggccct
     2581 cccggacctt ggttcgggga ttgtatccca gcatctccct tgccttgcgg atatctgcat
     2641 aagtttgtct tacgtccccg ggttgttccg gcagatattc cagaaccgct ttctttccgg
     2701 cctgcccttc gatttctcga atcagttcgg aaagggatat ggtttgagat tcacccaggt
     2761 tatagatttg gaagccgttc tcccgggaga aggctcccag cgttccttcg ataatatcct
     2821 cgatgtaggt atagtctcgg cgggaactgc cgtcgccgaa gagggagatt cttctgccct
     2881 caaagatcga tcggataaaa tgatggatgg ccatctcagg ccgctgccgg gggccgtaca
     2941 cagtgaaata ccggaggcat gcaatatcca tcccgtaaag gtgatggtag gtatggcaca
     3001 ggagttcccc cgccttcttg gtggcggcat agggggagat gggatggttc accgggtcct
     3061 cttcggaaaa gggaactcgg gaactgttgc cgtacaccga agaagaagaa gcgaagatga
     3121 agcgacgcac tccatgctgc cgacaggcct ccaggagata gatggttccc cggatgttca
     3181 cgtcggcgta aagagcaggg ttctgaagcg aaggccggac tccggcccgg gcggccaaat
     3241 ggaaaacccc atctatcttc tccttttcga atatctgtcc catcaatttt tcatcgcgca
     3301 ggtccccctc gattaatttg aattgcgaat tctggagggc gcggctcaga ttccggccct
     3361 tcaaggccgg gtcataaaaa tcatcgaaat tatccaggca gacaatccca tgtccgtcct
     3421 tcaggagcct ttccaccaaa tgggacccga tgaagccagc accgccagtc actagaacat
     3481 tcatggtaat tccctttttc aaatcccaaa ttgtaggggc gtcccgccgt gggcgccctt
     3541 tgcagcgggc ggccacatgg ggccgcccct acattttctc tcccgctatt agttcggcaa
     3601 tcacttcgat atggtacgtt tgtggaaaca tatccagcgg ctgaatccgt ttcaaattat
     3661 aacccagcga aaccagatgg gccaggtccc tggccaaggt cggaggctcg caggagacgt
     3721 acaggattct tcgggggtga agggaagcga gaggaatgag agcgctgtga gccccaacgc
     3781 gcggtgggtc cagaacaacc aaatccaccg ccccggtttc tctcttgatt ctcccgattc
     3841 cttcctctgc ggcgccagcc caaaaagtgc agttgcctat cagattcctc cgggcgtttt
     3901 cgcgggccga gtcaatggct tctatatcct gatccactcc ccataccttt ccgcccctga
     3961 gggccaaagg aagagtgaga ttccccgaac cgcagaaaag gtcgattccc ttctcttctc
     4021 tcttgagatc ggcccactcc agcacttttc caatcaggtt gcgattctgg tcgggattga
     4081 cctggacgaa agacttcccg ccggcttgca ggagaagcc
//
LOCUS       DASPRR010000237        11948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_542456, whole genome shotgun sequence.
ACCESSION   DASPRR010000237 DASPRR010000000
VERSION     DASPRR010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542456"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /gene="aroC"
                     /locus_tag="VEL68_09140"
     CDS             complement(<1..462)
                     /gene="aroC"
                     /locus_tag="VEL68_09140"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXZ36170.1"
                     /translation="MSGNTLGVLFRVTTWGESHGPAVGAVVDGCPPGLELAADDIQQQ
                     LNRRRPGTGQASSSRQEEDRAEILSGVYQGRTTGTPIALLIRNRDVKSSDYESLEDVF
                     RPGHGDWTYFKKYGIRDHRGGGRASGRETAARVAAGAVARKVIARAGIEVFA"
     gene            complement(444..1172)
                     /locus_tag="VEL68_09145"
     CDS             complement(444..1172)
                     /locus_tag="VEL68_09145"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXZ36171.1"
                     /translation="MNFILIGYRASGKTSAGKKLSGLLGLPFYDTDTLIQKQTGKTIR
                     QMVLEGGWPSFRQAEREAIASLAGKEGAVIALGGGAVLDPANVEVLKPRGLFIWLQAD
                     KETIQERLKGDRASAEQRPPLSNSVNGDVEEILRQRIPLYESIADLIFDTTGLSVEAV
                     AEKIMASLGNRKETGLAYSGISLFQNVAGAQFLENSSPKSPPTPLCKGGQGGFLGNFC
                     RRLRSRFLPRATVSPGEKRCRETR"
     gene            complement(1169..2452)
                     /gene="aroA"
                     /locus_tag="VEL68_09150"
     CDS             complement(1169..2452)
                     /gene="aroA"
                     /locus_tag="VEL68_09150"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXZ36172.1"
                     /translation="MCDPIEVQPVSGVNAAVHVPGSKSYTQRALVIASLAEGQSFLQG
                     PLVSEDTDYLIEALQVLGAQVLPSGKNLAITGTGGSIAPPRRPIFLGNNGTGARFLLS
                     MASLGNGDYHLTGSPRLCQRPMKPLLDALRSLGGASTCEGKEGYLPLTMHAQGIQGGK
                     ATFSEVESSQYISSLLISAPHARKDVEIEVKGFTASRPYIAMTLEVMKHFGVSVEREV
                     GKRYFLRSGQRYRGTSYAVEGDASSASYFFLAAAICRGTVRVSPMNRKTLQGDGKFLG
                     TLEELGCAVSSGGSCVEVEGGPLPAGDRVFDMGDMPDMVPTLAVLSAVRPGRTVIRNA
                     AHLRIKESNRLEALARELAKTGIRAEEREDGLVVEGGSPRGAEIETYNDHRIAMSFAM
                     LGLAVPGMRIQDGSCVRKSFPGFWEELGKLYGNRP"
     gene            complement(2496..3353)
                     /locus_tag="VEL68_09155"
     CDS             complement(2496..3353)
                     /locus_tag="VEL68_09155"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXZ36173.1"
                     /translation="MRTDKEQLFCLFGNPVGHSLSPLMHNAAYRAMNLAARYQAFLVP
                     DPETAVRLFREMGMDGASVTLPFKTSIMDHLDDLDENCRAIGAVNTVWVRDGKLLGSN
                     TDWIGVARSLKSHLDIRGKTLAILGSGGAARGAVFAVRKEGGNPVLVCRNPLKGKELA
                     RDFDCPLHPLSDLGSIKAHVLINTTPVGMGSDAESTPARRKDLVNFQWVMDMVYNPLR
                     TALLREAEAAGSGVISGLSMFVHQGAEQVRIWTGMEPPLELMTRIVKEELLRRSAGRG
                     DSRRVESPT"
     gene            complement(3350..4147)
                     /gene="aroD"
                     /locus_tag="VEL68_09160"
     CDS             complement(3350..4147)
                     /gene="aroD"
                     /locus_tag="VEL68_09160"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HXZ36174.1"
                     /translation="MICVPVMAESNREAVRMMKRGFASADMVELRLDRISRPALPALM
                     KAREGSLLVTNRRKEEGGFFKGYERDRVNLLAEAVNLGADYVDVEASTGKRWISRLKA
                     QIEGKGSATRMIISHHNFQRTPSWDGLVRMLDACRAYGAHAVKIVTFANSAEDNLRVL
                     QLIPRSRAEGQPIIAFCMGPQGRISRLLAPALGSFITYASLRREAESAPGQFTVAEMR
                     KMWRLLGCEGSPLLHSGEADMVFAQGTQDRGRRGRICSSRGEAPEKA"
     gene            complement(4144..5223)
                     /gene="pheA"
                     /locus_tag="VEL68_09165"
     CDS             complement(4144..5223)
                     /gene="pheA"
                     /locus_tag="VEL68_09165"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXZ36175.1"
                     /translation="MKRKTVEKIREEIEKKDRELLRLLNQRSSLAEGMGRVKRQLGRE
                     VYDPSREARILASLLGANEGPLPDEALSNIYREIFSSSRQLQSPLTVACLGPEASFSH
                     LAALAQFGKSPQIVLEKTIGQVFDRLEKGNASFGVVPFENSIEGSVKMTLDRMISTSL
                     KVRAEVFLRVSHCLMSCGKEREIKKVYSHPQALAQCREWLARNLPQAEPVATDSTATA
                     AMRVREDKRGAAIASSLASKVYGLPILAEGIEDHPINITRFLVLGNGESSPTGRDKTS
                     ILFGTPHVPGALHGTLGPFARAGINLLRIESYPIRDRVWEYLFFADFGGHLQERKVRE
                     CLRKVEGKTVFLKVLGSYPRGDDSR"
     gene            complement(5220..6314)
                     /gene="aroB"
                     /locus_tag="VEL68_09170"
     CDS             complement(5220..6314)
                     /gene="aroB"
                     /locus_tag="VEL68_09170"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXZ36176.1"
                     /translation="MNRIQVNLDRGSSASYEVHIGYQIMDRMALLLSRHGWPRRYFAV
                     MDSNVSALHGEKVLSILRSLGLQVEKLEFPGGEARKSIHTCAELAERLMERGADRTSA
                     LIALGGGVVGDLTAFLASIYMRGIPHVQVPTTILAQVDSSIGGKTGVDLPTAKNLLGT
                     FTQPKAVFIDLAFLETLPPREFTNGLAEIAKYGIIDDPELLDELDAGADTLRRRDPAF
                     LEGIISKSCRIKKEFVESDETDRGLRRILNFGHTVGHAIEAESGFAVSHGEAVSMGMC
                     TAVLLSERLRYLSSGDRERILSVLQGLGLPVRIPASLDTEGLLQGLKKDKKKEGAKIH
                     FILLKKPGQPFVNGGVPLPLVQETIEALKA"
     gene            complement(6311..7339)
                     /gene="aroF"
                     /locus_tag="VEL68_09175"
     CDS             complement(6311..7339)
                     /gene="aroF"
                     /locus_tag="VEL68_09175"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXZ36177.1"
                     /translation="MIIVMKNNAAEEQIRKVVEWIESIGYKAHLSQGVERTLIGAIGD
                     GRGKEQLNAIVSLPGVEKVVPILKPYKLASREFKASNTVVRVGNLEIGGPRFIIMAGP
                     CTVESEEQLMESAYVVKKGGAHILRGGAFKPRTSPYSFQGMEEEGLRLLKKAGEKTGM
                     PVVTEVMNTTEVELVEEYSDILQIGARNVQNFALLKKVGQARKPVLLKRGMMTTLEEL
                     LMSAEYILSAGNHQVILCERGIRTFETATRNTLDLSAVVVLKECSHLPVIVDPSHATG
                     HWKFVTPLARAAVAVGADGILIEVHPEPEKAVCDGLQSLKPEKFYQLMEEVSLLEQIL
                     RKEVRTHL"
     gene            complement(8040..9419)
                     /locus_tag="VEL68_09180"
     CDS             complement(8040..9419)
                     /locus_tag="VEL68_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ36178.1"
                     /translation="MGFRVLIIDDEPTLRESLQIALTASGYEVQTARTGEEGLTLFTQ
                     ENPDLILLDHWLPGINGDQVLQRIKEAEPEIPVIIMTAQGSVDLAVQSMKMGAFDFLV
                     KPFELDQIEALVQKGLERTRLKKELEWLRAQYREKFRSGGIVGASPKMREVVQLAERL
                     APGSETTVLIEGETGTGKELLAEYIHSLSPRSSFPFVPINCGAFPQELLESELFGYEK
                     GAFTGASEKGKMGKIESAEKGTLFLDEVGELPPPAQVKVLRVLEEKEYFKLGSVEKKK
                     ADVRFVAATNKDIEQEVRKGKFRDDLFFRLNVVRIRIPPLRERKEDVLALFRFFMARF
                     NEQFQKGFLRISPEAEGRILAYPWPGNVRELRNTVERIVLLERGDTILEKHLVFLGGK
                     DQERLKPSLRPAIPPQGIVLDEVMKEYILEALRMKKGNKIQAAKLLGISRSALLYRMD
                     KFGIKNGSE"
     gene            complement(9409..10908)
                     /locus_tag="VEL68_09185"
     CDS             complement(9409..10908)
                     /locus_tag="VEL68_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ36179.1"
                     /translation="MERKIRILVAEADLSFAKMIADSMKESGGSYELEAVSSGRDCLE
                     KLRQQKFDILLLDHDLPDGRGLDWLRRFNELGVGIPTIFITTQGDSRLAIEAMKEGVF
                     DYINRSAECAKAFPFVVHRAVEGYHLMVEKVRLQKELIETKNFLESVIDKAGDAISVI
                     DLEGKILHWNEGAERIYGSPREEALGKTLSHCLYAGDKKGLQEEEKTLRELLARVRKG
                     EVIPPMERKRRLRDGREIIAQMTLSPLKDADGRIIGATRICRDITHLKRAEERLVLAE
                     RLSSLGELTAGVAHELRNPLAGIKINTQVLARKKDLSETDRRVLESTQEGIEKIQKIV
                     DDMLHFARPKAAHFQEEDIYLVIDKSLSVLQAKLRRANISLVFNRDEKVPRVWIDLHQ
                     VQQVLINLMLNAIQAMEKGGTLTLRAFRESEGGVGVEIRDTGVGIPAANLKKIFDPFF
                     TTKSEGTGLGLSISMKILDNHRASLDVESEVGKGSVFTLHFPGESARGI"
     gene            complement(11004..>11948)
                     /locus_tag="VEL68_09190"
     CDS             complement(11004..>11948)
                     /locus_tag="VEL68_09190"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HXZ36180.1"
                     /translation="QLRLEEPVTALPGDRFVIRSYSPVFTIGGGEILDAFPARHKRLS
                     PQVKEEMAVLEKGSEEEKIKLRLLKAGAGGLSWADLVMRSNQLPSKLKSLVDGLVSKG
                     GLLRFNGDRLRYLHPRVMADLKQFSLDYLKEFHQKNPLQMGAVKEELKSKLPSQVDAR
                     LFNHLLSILTGEKRIALDKETVRLSSHAISLKEEEKDLRKKIVVLFSRTKLQPPTVKE
                     TLVELGVSESELKPVLQLLTKENVLVKVKEDLYFHRQALEDLEGKLVQFLQQNKEMTP
                     PQFKDISQVSRKFAIPLLEHFDGKKLTMRIGDKRVLRK"
BASE COUNT         2330 a         3581 c         3364 g         2673 t
ORIGIN      
        1 agcgaagacc tcgattcctg cccgggcgat gactttgcgg gccacggctc ccgcagccac
       61 gcgggcggcc gtttcccgcc ctgaggcccg cccgccgccg cggtgatccc ggatgccgta
      121 tttcttgaag taagtccagt ctccgtgccc tggccgaaaa acatcttcaa gggactcata
      181 gtccgagctt ttgacatccc ggttacggat gagcagggcg atcggggttc cggttgtccg
      241 gccttggtag acccctgaaa ggatttccgc ccggtcttct tcctgccggg acgaagacgc
      301 ctggccggtt ccgggtctgc gccggttgag ctgctgttga atgtcatcgg cggccagttc
      361 caggccggga gggcatccgt ccacgacagc tccaacggcc ggtccgtgag actcgcccca
      421 ggtggttacc cgaaagagaa cccctagcgt gtttcccgac atcttttctc tcccggtgat
      481 acggtagctc tcggtaaaaa ccgactcctc agccttctgc aaaaatttcc cagaaatccc
      541 ccctgccccc ctttgcaaag gggggttggg ggggatttgg gtgatgagtt ttccagaaat
      601 tgggccccgg caacattctg aaaaagggag attccagagt aggccagccc tgtttctttc
      661 cggtttccga gagaggccat gatcttttca gcgacggctt cgacgcttag ccccgtcgta
      721 tcgaagataa ggtcagcgat ggattcataa agaggaatcc gctgtctcag gatttcttct
      781 acgtcgccgt tcacggaatt tgaaagagga ggtctctgtt cggcgcttgc ccggtctcct
      841 ttcaggcgtt cttgaatcgt ttctttatcg gcctgaagcc agatgaataa gccccggggc
      901 ttcaaaacct ccacgttcgc ggggtccagg actgcccctc ctcccagagc gatgactgct
      961 ccttcttttc ctgcaaggct ggcgatggcc tccctttccg cttggcgaaa agacggccat
     1021 cctccttcga ggaccatctg ccgaatggtc ttcccggtct gcttctggat cagcgtgtca
     1081 gtgtcgtaga agggaaggcc aagaaggccg gaaagttttt tcccggctga cgtcttcccc
     1141 gaagcgcgat acccgatgag gatgaaattc atgggcgatt cccgtacagc tttcccagtt
     1201 cttcccagaa accgggaaag gattttcgca cacagctccc atcctggatg cgcatgcccg
     1261 gaaccgcaag gccgagcatg gcaaagctca tggcgatgcg gtgatcgttg taggtctcga
     1321 tctccgcccc tcgggggctc cccccctcga ctaccaaacc atcctccctt tcttctgcgc
     1381 ggatgccggt tttggccaat tccctggcca gcgcctccag acggttgctc tctttgatcc
     1441 gaaggtgagc cgcgttgcga atgacagtcc ggcccgggcg gacggcggaa aggacggcta
     1501 gggtgggaac catgtccggc atatcgccca tgtcgaagac ccgatcccct gcgggaagcg
     1561 gtcctccctc tacctcaacg caggacccac cggatgagac ggcgcacccc aattcttcga
     1621 gggtaccgag aaacttccca tctccctgca gggtcttgcg attcatgggg gaaacccgaa
     1681 ccgttccccg gcagattgcg gcggccagga agaaatagga agcgctggag gcgtcgccct
     1741 cgacggcata agaggttccc cggtagcgct gtccgcttct caggaagtac ctcttcccca
     1801 cctccctttc cacagagacc ccaaagtgct tcataacctc cagggtcatg gcgatgtacg
     1861 ggcgtgaggc ggtaaagccc ttgacttcga tttccacatc cttccgggcg tggggagcgc
     1921 tgatcagaag ggaggagatg tactggctgc tttccacttc ggaaaaggtc gccttccccc
     1981 cctggatccc ctgcgcatgc atggtcaggg gaagatagcc ttccttcccc tcgcaggtgg
     2041 aggctccgcc caaggaacgg agggcgtcta aaaggggttt catgggcctc tggcagagac
     2101 ggggagatcc ggtaagatga tagtctccgt tccccaaaga agccatgctg aggaggaatc
     2161 gggctccggt tccgttgttc cccaaaaaga tcggtctcct cggaggggca atactccccc
     2221 ctgttccggt tatggcaagg ttcttgcctg aaggaagtac ctgcgcgccg aggacctgca
     2281 gggcctcgat caagtaatcc gtgtcttcag aaaccagggg gccttggagg aaagactgtc
     2341 cttcggccag ggaggctatg accagggccc tctgggtata gctcttggaa cccggaacgt
     2401 gcaccgcggc attgaccccg gaaacgggtt ggacctcaat cgggtcgcac attaagggcc
     2461 tccgttcttc ttgataaata atttgtaagg gcgattcatg tcggagactc gactctacga
     2521 gaatcgcccc tacctgcgga ccggcggaga agctcttcct tgacgatgcg ggtcatcagc
     2581 tccaggggcg gctccatccc ggtccagatg cggacctgct ccgctccctg gtgaacgaac
     2641 atggagaggc cgctgatcac cccgctcccc gctgcctccg cttccctcag gagcgctgtc
     2701 cgcagaggat tgtaaaccat gtccatcacc cactggaaat tgaccaggtc tttcctccgg
     2761 gcaggggtgc tttccgcatc cgatcccatg cccaccggag tggtgttgat caagacatgg
     2821 gccttgatgc tccccagatc ggataagggg tggagagggc aatcgaagtc ccgcgccagc
     2881 tcttttcctt ttaacgggtt gcggcatacc aacacagggt ttcccccctc tttccgtacg
     2941 gcgaagaccg ccccccgggc ggcgccgccg gaacccagga tggcgagggt tttaccccgg
     3001 atgtccaaat ggctcttgag actccgggcc accccgatcc agtcggtgtt tgaccctaaa
     3061 agctttccgt cgcgaaccca aaccgtgttg accgccccga tggcccggca attctcatcc
     3121 aaatcatcca gatgatccat gatcgacgtt ttaaagggaa gagttacgct ggccccgtcc
     3181 atccccattt cccgaaacag gcgcaccgcc gtttccgggt ccggcacaag aaaggcctgg
     3241 taacgagcgg caagattcat cgcccggtag gccgcattgt gcatcagcgg cgagaggctg
     3301 tgccccacgg gattgccgaa aaggcagaag agttgttctt tgtcggtcct catgccttct
     3361 ctggggcctc ccctcgactt gagcaaatcc gtcctctccg gccacggtct tgcgtccctt
     3421 gagcgaagac catatcggcc tccccggaat gcaaaagggg cgagccctcg cagccgagca
     3481 gccgccacat cttgcgcatt tcggccacgg tgaattggcc gggtgccgat tcggcctctc
     3541 ttcgcaggga ggcgtaagtt ataaaagagc ccagagccgg ggccaggagc cggctgatcc
     3601 gtccctgagg gcccatgcaa aaggcgatga tcggctgccc ttcggcccgc gaccggggga
     3661 tgagctggag aacccgaaga ttgtcctcgg ccgagttggc aaaggtgacg atctttaccg
     3721 cgtgggcccc ataagcccga caggcgtcga gcattctcac caggccgtcc caggaggggg
     3781 ttctctgaaa attatgatgc gagatgatca tccgggtagc tgatccctta ccctcgattt
     3841 gggctttcag ccggctgatc cagcgtttcc ccgtgctcgc ttccacgtca acgtaatccg
     3901 cccccaggtt caccgcctcg gccagcaaat tcactcgatc cctctcgtaa cccttgaaga
     3961 agcccccttc ctccttgcgc cggttggtga ccaggagact cccttcccgc gccttcatca
     4021 gggcgggaag agcgggccgg gaaatccggt ctaagcggag ctcgaccata tccgccgacg
     4081 cgaatccgcg cttcatcatt cgtaccgctt cacggttgct ttcggccatg actgggacac
     4141 agatcatcgg ctgtcatccc cccttggata ggaccccaga actttgagaa agaccgtttt
     4201 cccttccacc ttgcggaggc attcgcgaac ttttctctct tggagatggc cgccgaaatc
     4261 ggcgaagaag aggtactccc acacccgatc ccggatgggg taggattcga tcctaaggag
     4321 gtttatccct gctctggcga aggggcccag ggtcccgtga agggctcccg ggacatgagg
     4381 ggtgccgaat agaatcgaag tcttgtccct gcccgtgggg ctgctttccc cgtttcccag
     4441 gactaggaaa cgggtgatat tgatgggatg gtcctcaata ccttcggcca ggatcggaag
     4501 cccatagacc ttggaggcca gagagctggc gatggcagcc ccccttttat cctcccggac
     4561 ccgcatcgcg gctgtggctg tgctgtccgt ggccaccggc tcagcttggg gaagattccg
     4621 cgccagccac tcccggcatt gggccagggc ttgggggtgg gaatagacct tcttgatttc
     4681 tctttctttt ccgcaggaca tcaagcagtg ggagaccctt aggaagactt cggcccggac
     4741 cttcaaggaa gtggagatca tccggtcgag ggtcatcttg accgatccct cgattgaatt
     4801 ttcgaaggga acgacaccaa aggaggcgtt tcccttttcc agtcggtcaa agacctgccc
     4861 gatagttttc tccagcacga tttggggact ctttccgaac tgggccaggg cggccagatg
     4921 ggaaaaagac gcctccggcc cgaggcaggc cacggtaagg ggcgactgca gctgtctgga
     4981 agaagagaaa atctcccggt agatgttgct cagggcctca tccggcaggg gcccctcgtt
     5041 ggccccgagc agggaggcga gaatccgggc ctcccgggag ggatcataaa cctccctgcc
     5101 caattgcctc tttacccggc ccatcccctc cgccagggag cttcgctggt tgaggagccg
     5161 gaggagctcg cggtcctttt tttcgatttc ctcccggatt ttttccaccg tttttctttt
     5221 catgccttca gggcctcgat ggtttcctga accaagggca gagggactcc tccgttcaca
     5281 aaaggctgcc ccggcttctt cagcagaata aaatgaatct tggcgccttc tttcttcttg
     5341 tccttcttca gcccttgcag gaggccctca gtgtccaggg aggcggggat gcggaccgga
     5401 aggcccaggc cctgtaagac cgaaagaatt ctctcccggt cccccgagga gaggtatctc
     5461 agccgctcgg agagaaggac ggcggtgcac attcccatgg atacggcttc cccgtgggat
     5521 acggcgaacc cggactcggc ctcgatggca tgtcccacag tgtgcccgaa gttgaggata
     5581 cggcgcagcc cccggtccgt ttcgtcgctc tccacgaact cctttttgat ccggcaagat
     5641 ttggagatga ttccctccag aaaagctggg tcccgccgtc gcagggtgtc ggcgcctgcg
     5701 tccagctcgt ccaggagctc cggatcgtcg atgatcccgt acttggcgat ttccgccagc
     5761 ccgttggtga actctcgggg ggggagagtt tccagaaagg ccaggtcgat aaaaaccgcc
     5821 ttgggctggg tgaaagtccc caggaggttc ttggccgtgg gaagatcgac gccggttttg
     5881 ccgccgatgc tgctgtccac ctgggcgaga atggtggtgg gcacctggac gtgggggata
     5941 cccctcatgt aaatggaggc gaggaatgcg gtcaggtcgc ctaccacgcc gccgcccaga
     6001 gcgatgaggg ccgaagttcg atcggccccc cgctccatca atcgttccgc caattcggcg
     6061 caggtgtgaa tgctcttacg ggcctcccct cccgggaact ccagcttttc cacctggagc
     6121 cccaggctcc ggaggatgga caagaccttt tccccgtgaa gggcggatac attggaatcc
     6181 atgaccgcaa aataccgccg cggccagcca tggcgagaaa ggagaagagc catgcggtcc
     6241 atgatctggt aaccgatgtg gacctcgtaa gaagcagagg aacctctgtc caagtttacc
     6301 tggatgcgat tcataggtgc gttcgaacct cctttctcag gatctgctca agaagggaca
     6361 cctcttccat gagctggtag aacttttccg gctttaacga ctggaggccg tcacagactg
     6421 ctttctcggg ttcgggatgg acctcgatga gaatcccgtc ggctcccacg gccacggcgg
     6481 ccctggccag aggggtgaca aacttccaat gccccgtggc atggctggga tcgacgatca
     6541 ccggcaggtg agaacactcc ttgagaacga ctacggcgct caggtcgagg gtgttgcggg
     6601 tggcggtttc aaaggtgcgg attccccttt cgcagaggat gacctggtgg ttgcccgctg
     6661 acaaaatgta ttccgctgac atgagaagct cttcgagggt ggtcatcatc ccccgcttta
     6721 aaagaacggg tttccgggcc tggccgacct tcttgagaag ggcgaaattc tggacattgc
     6781 gggccccgat ttggaggatg tcggagtatt cttccaccag ctccacctcg gtcgtattca
     6841 tgacctcggt caccaccggc atcccggtct tctccccggc tttcttcaag agccgtaggc
     6901 cctcttcttc catcccctga aagctgtagg gggaggtccg cggcttgaag gcgccgcccc
     6961 tcaggatatg ggcgccgccc ttcttcacca cgtaggcgct ttccatgagc tgttcttcgc
     7021 tttccacggt gcagggtccg gccatgatga tgaatcgggg tccgccgatc tccaagttcc
     7081 ccacccggac caccgtgttc gatgccttga attcccggct ggccagctta tagggcttca
     7141 ggatggggac caccttttcc acccccggca gggagacgat ggcattcagc tgctctttcc
     7201 cccggccgtc cccgatggcc ccgatgaggg tcctttccac cccctgggaa agatgagcct
     7261 tatagccgat ggattcgatc cactcgacta ccttacgaat ctgttcttcg gcggcgttgt
     7321 ttttcatgac gatgatcatg gctttctccc tccaacgaaa aaggccacgg ggtttttgcc
     7381 accatggcct tttcgagaaa gaagaaaata aaaaagccat ggatagcgtc ttttcatcta
     7441 cccatggctt gtttttttga ttaggacctt tggtctaggt catcttggcc gatgggcaga
     7501 cccctccgaa ataataataa aaccaccagc caaaattctt cgtgatcaag ggtctgtcca
     7561 tcgagttttc tttcttggaa ttttaatcat tcaaacacag gaatgattcc ttgtcaagaa
     7621 aaaaattttc tctggaagaa attttctttt gtccaccttc acgattgcga tcgcattttc
     7681 ttctaaaaag caagcttgtg gcccggaagc aagggcgttg cgcgcaaaaa gcatcggcgg
     7741 gaaaagaaag ccactcgaag ggtcacgcgt gtttccgcag aatgcggcgg gcgcatcggg
     7801 cgcgccgccg gtggtcccac atcggaatca cctggttctt gggggaaaga caagcttgcc
     7861 cgacctgacc aagaaataaa gggtgcctca tggtttttgc gcgcaacgcc cttgtttccg
     7921 ggccgggcaa tgcccaagat tcagtctaac ttcacgccta cttggcgaaa agtagaggtg
     7981 ggaaaaattt actaatcctg tctttactcc gaactccgaa ctcaaaactc cgaatcccat
     8041 tactccgaac cgtttttgat cccgaattta tccatccggt agagaagggc tgagcgggaa
     8101 atgccgagca gcttggcagc ctgaatttta tttcccttct tcatcctcaa ggcttcgagg
     8161 atatactctt tcatcacctc gtccaggaca atcccctgcg gggggatggc cggccggaga
     8221 gagggtttaa gccgctcttg gtctttgccg cccagaaaaa cgaggtgttt ctccaagatc
     8281 gtgtcccccc tttccaggag gacgatcctc tccaccgtgt tgcgcagctc ccgcacattt
     8341 cccggccagg gataggccag gatgcgccct tccgcctcag gggagatgcg aaggaatccc
     8401 ttctggaact gttcgttgaa ccgggccatg aaaaatcgaa agagagccag gacatcctct
     8461 tttctctccc gcaagggggg aatccggatt cgcaccacat tgaggcggaa gaataggtca
     8521 tcccggaatt ttcccttcct cacttcttgt tcgatgtcct tgttcgtggc ggccacaaag
     8581 cggacatcgg ccttcttctt ctctacgctc cccagcttga aatactcctt ctcctccagg
     8641 acccttagga ccttcacttg ggccggcggg ggaagctccc cgacctcgtc taggaaaagc
     8701 gtgccctttt ctgcggattc gatcttcccc atcttcccct tttccgaggc tccggtgaag
     8761 gcgccttttt cgtaaccgaa tagctcgctc tccagaagct cctgggggaa ggccccgcaa
     8821 ttaatgggga cgaaggggaa ggaagagcgg gggctcaaag agtgaatata ctcagccaaa
     8881 agctccttgc ccgttcccgt ttccccctcg atgagcaccg tggtttcgga accgggggcg
     8941 agcctttccg ccagttgaac cacttccctc attttggggc tggccccgac gatccccccg
     9001 gagcggaact tttcccggta ctgggcccgg agccattcca gttccttttt caggcgggtt
     9061 cgttccaggc ctttctggac cagggcctcg atctgatcca actcgaaggg cttcaccagg
     9121 aagtcgaagg cccccatctt catggactgg accgcaaggt ccaccgagcc ctgggcggtc
     9181 atgatgatga ccggaatctc cggttcggct tccttgatcc tctggaggac ctgatctccg
     9241 ttgatcccgg gaagccagtg gtccaaaaga atgaggtccg ggttctcctg ggtgaagagg
     9301 gtaagccctt cctcccctgt ccgggcggtt tgaacttcat accccgaagc ggttagggcg
     9361 atctggaggg actcccggag ggtgggctcg tcatcgatga tcaggaccct aaatcccacg
     9421 ggccgactcc ccggggaaat gaagagtgaa gaccgatccc tttcccactt cgctttccac
     9481 atccagagaa gcgcggtggt tatccaggat tttcatgctg atggaaagac ccaggcccgt
     9541 tccttcggac ttggtggtaa aaaagggatc gaaaatcttc ttcagattgg ccgcgggaat
     9601 cccaactccc gtatcgcgga tttctactcc cacaccgccc tcgctttccc ggaaggcgcg
     9661 gagggtcagg gtcccgccct tctccatggc ctggatggca ttgagcatga ggttgatcag
     9721 gacctgctgg acctggtgaa ggtcgatcca gacccgggga actttttcgt cccggttgaa
     9781 aaccagggaa atgtttgctc tccggagttt ggcctggagc acggacaggc tcttgtcgat
     9841 caccaggtag atgtcttctt cctgaaagtg agcagccttc gggcgggcga aatggagcat
     9901 atcatcgacg attttctgga tcttttcaat cccttcctgg gtactctcca gaacccgccg
     9961 gtcggtttcc gagaggtctt tcttccgggc gaggacctgg gtgttgatct tgatcccggc
    10021 gaggggattt cgcagctcat gggccacccc ggcggtgagt tcgcccaggg aagataatct
    10081 ttccgccagc accaaccgct cttcggccct tttcaaatga gtaatatccc ggcagatgcg
    10141 ggtggctccg atgattctcc catcggcgtc cttcaggggg gagagggtca tttgggcgat
    10201 gatctccctt ccgtcccgga gccgcctctt tctttccatc ggggggatca cttccccctt
    10261 cctcaccctt gcaaggagct cacgaagagt tttctcttct tcctggaggc cttttttatc
    10321 cccggcgtag aggcaatggg ataaggtttt ccccagggcc tcttccctgg gggagccgta
    10381 gatccgttcg gccccttcat tccagtggag gattttccct tccaggtcga tcaccgagat
    10441 ggcatcgccg gccttatcga tcaccgactc gaggaagttc ttagtttcga tcaattcctt
    10501 ctgcagccgg actttttcga ccatcaaatg gtatccctcg acggcccggt gaacgacgaa
    10561 aggaaaagcc ttggcacact ccgcggaccg gttgatgtag tcgaaaactc cttctttcat
    10621 ggcttcaatg gccaggcggg aatctccctg ggtggtgatg aagatggtag ggatcccgac
    10681 gcccagttca ttgaaacgcc ggagccagtc caggcctctt ccatcgggga ggtcatggtc
    10741 aaggaggaga atgtcaaatt tttgctgccg aagtttctcc aggcaatccc ggccggagga
    10801 cactgcctcc aattcatagc tgccgcccga ctccttcatc gaatcggcga tcatcttggc
    10861 gaaggacagg tctgcttcag cgaccaggat ccttattttc ctctccaagc ttcttccttc
    10921 ttcttatttt tttgtcgatt ttgaattgag cattcggaat gtccggggtc tttccaatcc
    10981 gcaatccgca ttccgcaatc gggttatttc ctcaacaccc tcttgtcccc gatccgcatg
    11041 gtcagcttct tgccgtcaaa atgctccagg agggggatgg cgaacttacg cgacacctgg
    11101 gagatgtcct tgaactgggg aggggtcatc tccttattct gctgtaagaa ctggaccaat
    11161 ttcccctcca ggtcctccag ggcctggcga tggaagtaga ggtcttcttt caccttcacc
    11221 agcacgtttt ctttggtaag aagctggagg accggcttga gttcactttc cgagaccccg
    11281 agctcgacca gagtttcttt taccgtgggg ggctggagct tggtccggga gaagaggacc
    11341 acgatcttct tgcgcaggtc cttttcttcc tctttcaaag agatcgcgtg ggaggagagg
    11401 cggacggtct ccttgtccag ggcgatcctt ttttccccgg tcagaatgga tagaagatga
    11461 ttgaagagcc gggcgtccac ctgggacggg agcttggatt tcagctcttc tttcacggcc
    11521 cccatctgga gggggttctt ctgatggaat tccttcaggt agtccaggga gaactgcttc
    11581 agatcggcca tgacccgggg atggagatag cgcaggcggt ctccgttgaa acggaggagc
    11641 ccacccttgg agaccagacc gtccaccagc gacttcagct tggagggcaa ctggttggag
    11701 cgcatgacca gatcggccca ggacagcccg cccgcccccg ccttgagcag gcggagcttg
    11761 atcttctcct cctccgagcc cttttccagg acggccatct cctccttgac ctgcggggaa
    11821 agacgcttgt gccgggcggg aaaggcgtcc aaaatctctc cccccccgat ggtgaaaacg
    11881 ggcgaataac tccggatcac gaaccggtcc ccgggaagag cggtcacggg ttcctccagc
    11941 cgcagctg
//
LOCUS       DASPRR010000238         8575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_861686, whole genome shotgun sequence.
ACCESSION   DASPRR010000238 DASPRR010000000
VERSION     DASPRR010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861686"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VEL68_09195"
     CDS             <1..257
                     /locus_tag="VEL68_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXZ36181.1"
                     /translation="RQKMDPGEADILILDEINNALHLKLVDLEQVLDLIDHKPPGLHL
                     VLTGRDAHPQVIERADTVSEIAELKHAYRKGIEPQPGIDY"
     gene            complement(321..752)
                     /locus_tag="VEL68_09200"
     CDS             complement(321..752)
                     /locus_tag="VEL68_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HXZ36182.1"
                     /translation="MPYRFLEELATADIAFIAEGNTLAETFIAAADATLNVMVETLDA
                     IFPRETKEFALDNERLDLLLFDFLQELIYYKDAQKLMLRVGRVQIKEKEEKFFLRANA
                     QGEKLDPERHHPRVDVKAVTLHRFRLEKTDRGWEAFVILDI"
     gene            complement(759..1643)
                     /locus_tag="VEL68_09205"
     CDS             complement(759..1643)
                     /locus_tag="VEL68_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXZ36183.1"
                     /translation="MEIRTPITLITGPLGSGKTTLLRHVLSLRPRKLAILINEFGEIA
                     IDSKVLEGKSIRIAELANGCVCCSLQGEFEAAVNETLDTVDPERIIVETTGVAEPDAL
                     VFDIQDNLPRVRLDGVITIADADALARYPQLGHTARMQLEAADMVILNKEDLVSPEAL
                     ELAREKLQRINGAASIFAAQRCRVDPDLLFGIGRERNLRPPEHLHQPGVESFFFRTPA
                     ELDRECFEKVAACLGQKVHRAKGFVRFPEGSYLFNFVSGRWELEPFPGENTVLVFIGQ
                     GLRERESEILARLRDCGG"
     gene            complement(1758..3215)
                     /locus_tag="VEL68_09210"
     CDS             complement(1758..3215)
                     /locus_tag="VEL68_09210"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HXZ36184.1"
                     /translation="MSVPKALRQVSETVWEIPPSYKEGMRVPARVYATEKLLEEMDEA
                     VYDQITNVATLPGITRYALCMPDGHFGYGFPIGGVAAMDEEEGVISPGGIGFDINCGM
                     RLVTTNLTYDEVKPHLKKLVDRLFVKVPAGVGSTGFIKISRNEFRRVVEEGARWCVRN
                     GYGWEEDLERTEESGCVSGADSSKISEKAIDRGFNQIGTLGSGNHYLEIQVARKENIL
                     DPELARRFGITIPDQVVVMFHCGSRGFGHQAATDYLQVFLRVMEKKYGIKVLDRELAS
                     APFDSPEGKDYFSAMKCGMNMSFANRQVITHRIREVFSDLFGRSPEDLGMHVVYDVAH
                     NTAKLERHWIDGKARNLLVHRKGATRAFGPGMEGLPACYQDVGQPVIIGGSMETGSYL
                     LAGTPGGSDTFYSTAHGSGRTMSRTKARRLWRGKQLQEEMEGRGIHVRTASWAGLAEE
                     AGGAYKDIDEVILATERAGISKRVVRFIPVGNIKG"
     gene            complement(3484..3888)
                     /locus_tag="VEL68_09215"
     CDS             complement(3484..3888)
                     /locus_tag="VEL68_09215"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXZ36185.1"
                     /translation="MNPRVLQEAVSLGQKLRHIFVATSDRSGLPHVAAASQLALSPEG
                     RVEVGAWFCPGTVANLQENKRISVVIWNPKKDLGFQLLGETEKVEELAIMDGYAPEEE
                     KGPPLPQIKRQLTVRVEKIIDFRHAPHSDREE"
     gene            complement(4039..6168)
                     /locus_tag="VEL68_09220"
     CDS             complement(4039..6168)
                     /locus_tag="VEL68_09220"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HXZ36186.1"
                     /translation="MKTRRIPDKVMKRDGAIVLFTPEKIQYAIYRAAFEVLQDSARAG
                     KIAKRLVDIVVQNLAEKYRDKPLHVEAIQDMVETVLMNQGYSQIAKSYILYRERRSEI
                     RLAKSVLGMKDDLKLPLNTMEVLKRRYLLKDDQRRIIETPSMLFQRVAHHVAQGEKNF
                     SSSVTPEQAEEKFYQMMRNLEFMPNSPTLMNAGASLGQLSACFVLPVEDSVDGIFGAL
                     TSMAKIHQTGGGTGFNFSRLRPKKDIVASTKGEASGPVSFMSIFDRATEVIVQGGRRR
                     GANMGVLRVDHPDIVEFIEAKIEKKRFSNFNLSVGVTDLFMKAVLANRDFDLINPRTQ
                     ERGNRVKARMIFDLIVNAAWLTGDPGLIFLDEINRKNPTPQVGPIEATNPCGELPLLP
                     YESCNLASINLARMVKGRGVDWKKLKEYVHWGVRFLDDVIEVNKFPLPQIEQMTFANR
                     KIGLGVMGFADLLIRLGIPYGTKDSVRFADKLMRFIHRESVKASSVLAEERGVFPNFP
                     KSVYPSRNLRMRNATVNTIAPTGTISIIAGCSSGIEPLFAVSFIRNVLSGTQLFEVNP
                     FFEEIARSRGFYSKELFAQIAHSGSLQQIKGIPADIKKVFVTAFDVTPHQHLEVQAAF
                     QKLTDNSVSKTINLPRESTVEDIREIYLSAYRLKCKGITVYRYGSKEEQVLSFGYGEG
                     ERPVVHPDLLTVESEYSGGCSSSACLF"
     gene            complement(6387..7655)
                     /gene="larA"
                     /locus_tag="VEL68_09225"
     CDS             complement(6387..7655)
                     /gene="larA"
                     /locus_tag="VEL68_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HXZ36187.1"
                     /translation="MATKEYKFKYGRKEVRFDLDPRLVIGELTIRDYPPLKDPVAEVQ
                     KAIRNPIQSKPFREIVKPGQTVCFLVNDPTRIANSHVFMPILLDELNAAGIPDKDMFI
                     MFSVGAHRMLPEEEMAQMAGAEAARRVKMYNSDAHDPSQFKFAGRTSRGNDVYFHKRA
                     MEADHIVCTGSVVYHFFSGFGGGRKALFPGVTRYETICRNHALMLEPGAVTGRLKGNP
                     AYEDQVEGTEMKRPSFLLNVVLNEKKEFLKVYGGDYIQAHLEACKFVETIYGAPISGL
                     ADLVIATCGGYPKDINVYQLQKTMDNAWLAVREGGVVIILGECEEGVGDNLYLEWMKK
                     HKTPERIEAEIRAHFVVGGHKAYAVTRLMKKAQFILISGLEPELARTLLFTPAKDMKE
                     ALQMAFAKVGPKPRVLLMPQGSLTVPIPKG"
     gene            7880..>8575
                     /locus_tag="VEL68_09230"
     CDS             7880..>8575
                     /locus_tag="VEL68_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36188.1"
                     /translation="MSDKVQPFPWMPILFLTAIFYLNFTSRVILAPLLPVIENDLGIG
                     HGEAGSLFLYLACGSGVGLVGSGFVSSRLTHRKTLSLSAIMVGTVLSVISASSSLGLM
                     RAGLVFAGIFAGLYMPSAIATLTEVASQEHWGKALAVHELGPNLGFITVPLLAEALLK
                     FFSWRESLALVGVACIVVGVLFLFFGKGGKHKGEPPRFQSMSKIVKSPSYWVMISLFT
                     VSIGSSVGLYSMIP"
BASE COUNT         1702 a         2516 c         2348 g         2009 t
ORIGIN      
        1 cccgccagaa gatggacccg ggggaggcag atattctcat cctcgatgaa atcaacaacg
       61 ccctccacct taagctggtg gacctggagc aggtcttaga cctcatcgat cacaaacccc
      121 ccggcctgca cttggttctg accgggcggg acgcccaccc tcaggtcatc gaacgggcgg
      181 atacggtcag cgagatcgcc gaattgaaac atgcctaccg gaagggaatc gagccgcagc
      241 caggaatcga ttactgaaca attaccaatg ttcaatgttc attgttcatt gatcactgtt
      301 tattgagttt tgtcccccct tcatatatcc agaatgacaa aagcctccca tcccctgtcc
      361 gtcttttcca gccggaaacg gtgaagggta accgccttca catccacccg aggatggtgg
      421 cgctccgggt cgagtttttc tccctgcgca ttggcccgga ggaagaattt ttcctccttc
      481 tccttgatct gaacccggcc gacccgcagc attaatttct gcgcatcctt gtagtagatc
      541 agctcctgga gaaaatcaaa gagcagaagg tccagccttt cgttatcgag ggcgaattcc
      601 ttggtctcgc gggggaagat ggcatctagg gtctccacca tgacattcag cgtggcatcg
      661 gcggcggcga taaaggtctc tgccagggta ttcccctcgg cgataaaggc aatgtcagcc
      721 gtagccagct cttccaggaa tcggtagggc ataccgagtt atcctccgca gtcccgaagc
      781 cgagccagga tctcgctctc ccgctcgcgg agcccctgcc cgatgaagac caggaccgta
      841 ttttctccgg gaaaaggttc cagctcccat cgaccggaga caaagttgaa aagatacgag
      901 ccctcgggaa aacggacaaa tcccttggcc cggtgaacct tttggcccag gcaggcggca
      961 accttctcga agcattcccg gtccaattcg gccggggttc gaaagaaaaa ggattcgacc
     1021 ccgggctggt gaaggtgctc gggcggtcgc aggttccgct cccggccgat tccaaagagg
     1081 aggtcagggt ccacccgaca gcgctgggcg gcaaagatag acgccgcccc gttgatccgt
     1141 tggagcttct ccctcgctag ctccagggct tccggagaaa ccaggtcctc cttgttcaaa
     1201 atgaccatgt ctgcggcctc gagctgcatc cgggcagtgt gacccagctg gggatagcgg
     1261 gccaaggcat cggcatcggc gatggtaatg actccgtcga gccgaactcg gggcaggttg
     1321 tcctggatgt cgaagaccag agcgtcgggt tcggcgacgc cggtggtctc cacaatgatc
     1381 ctttcggggt caaccgtgtc gagcgtttcg ttcaccgcag cttcgaattc cccctgcagc
     1441 gagcagcaga cgcatccgtt ggccagctcg gcaatgcgga tggactttcc ttcgaggacc
     1501 ttgctgtcaa tggcgatctc cccgaattcg ttgatgagaa tggcgagctt ccggggtcga
     1561 aggctcaaaa catggcggag aagggttgtt ttgccgcttc ccagggggcc ggtgatcaaa
     1621 gtgatggggg tacggatttc catttcgtag atcagccctt cttgcagaaa aggccggaag
     1681 gcgggattcg gagtgcggca ggggaatcat caggaggaag tttcgattcc aacgctcgct
     1741 catcgccggt ttcccgatta tccttttata tttcccacgg ggataaaccg caccacccgt
     1801 ttgctgatgc ccgcgcgctc cgtggccagg atgacctcat cgatatcctt gtaggccccc
     1861 ccggcctcct cagccaagcc cgcccaggat gcagtgcgga cgtggatccc ccggccttcc
     1921 atctcctcct ggagttgctt ccccctccag agccggcgcg ccttggtgcg gctcatcgtc
     1981 ctcccgctgc catgagccgt gctgtaaaag gtgtcgctgc cgcccggggt ccctgccaga
     2041 agataagagc cggtttccat gctgccgccg ataatcaccg gctgccccac atcctggtag
     2101 caggccggaa gcccttccat ccccgggccg aaggcccgcg tagccccctt gcggtgaacc
     2161 aggaggttcc tggcctttcc gtctatccag tgccgctcca acttggccgt gttatgggcc
     2221 acgtcataga ccacgtgcat tcccaggtct tccggggacc tgccgaaaag gtcggaaaaa
     2281 acttcccgaa tccgatgggt gatcacctga cggttggcaa aggacatgtt cattccgcac
     2341 ttcatagccg aaaaataatc cttcccttcc ggggaatcga agggggcgct ggctaactcc
     2401 cggtccagga ccttgattcc gtactttttc tccatgaccc tcagaaatac ctggaggtag
     2461 tccgtggcag cctgatggcc gaacccccgg ctgccgcagt ggaacatgac cacgacctga
     2521 tcgggaatgg tgatcccgaa ccgccgggca agctccgggt cgaggatgtt ttccttccgg
     2581 gccacctgaa tctccaggta gtggttgcct gatcccaggg tgccgatctg attgaagccc
     2641 cgatcgatgg ccttctcgct gatcttcgag gaatccgcac cgctgacaca ccccgattcc
     2701 tcggttcgtt ccaggtcttc ctcccatccg tagccgtttc ggacgcacca ccgggctccc
     2761 tcctcgacga cccggcggaa ttcattgcgg gatatcttga taaatcccgt gctccccact
     2821 ccggcgggga ctttcacgaa tagccggtcc accaattttt tgagatgggg tttcacttcg
     2881 tcgtaggtga gattcgtggt tacgagacgc atcccgcagt tgatgtcgaa cccgatgcct
     2941 ccgggagaaa tgaccccctc ttcctcatcc atggccgcca ctcccccgat gggaaacccg
     3001 tagccgaagt ggccgtcggg catacaaagg gcataccggg tgatcccggg gagggtggcc
     3061 acgttggtga tttggtcgta aaccgcctcg tccatttcct cgaggagctt ttcggtggcg
     3121 tagacccgcg caggaacgcg catcccctcc ttgtaagagg ggggaatctc ccataccgtt
     3181 tctgagacct gccggagggc cttggggacc gacacggttc aaacctccag gttagataaa
     3241 accttcaacc ctggggacgc aggcgctgag ccgtcactcc cgcgaaagcg ggagtccaga
     3301 atgccttgaa aagactggat tcccgcttcc gcgggaatga cactcctggc ctgaaaaagg
     3361 ttttttgcgc gatctgctaa atccatcggg ttttttaaaa cttcagccat ccgtttcctt
     3421 ttcgttatag aacggttctt gaacccctat ccgggtcgcg ccagaatctc ccggtcctta
     3481 gttttattcc tctctatcgc tgtgcggggc gtgccgaaaa tcgatgattt tttccacccg
     3541 cacggtgagc tgtctcttta tctgaggaag aggaggcccc ttctcctcct ccggagcgta
     3601 tccatccata atggccagct cttccacttt ttccgtttcc ccaagaagct ggaagcccaa
     3661 gtctttttta ggattccaga ttaccactga aatccgctta ttttcctgta agttggctac
     3721 cgtgcccggg caaaaccagg ctcccacctc gacccggccc tcgggggaaa gggctaattg
     3781 ggatgcagcc gcaacatggg gcagtcctga tcggtcagat gtagcgacaa agatgtgccg
     3841 gagcttctgg cccagggaga cagcttcctg aaggactctt ggattcatct ctcggtctcc
     3901 tttccattta gatgatcttt ttgccgcgaa gaagatatgc aaagagccta gggcaaggag
     3961 catggagtat ggagtgtgga gtctcaggga aacccatcac cccattgctt atgccctata
     4021 acgccctttg ctcttccttc agaagaggca agccgaagaa gagcatcccc cggagtactc
     4081 cgattcaacg gtcagaagat cgggatgaac caccggtctt tcgccttcgc cgtatccgaa
     4141 agacaagacc tgttcctcct tactcccgta ccgatacacg gtgatcccct tgcatttcag
     4201 ccggtaggcc gacaggtaga tttcgcggat atcctcgacc gtcgattccc ggggcagatt
     4261 aatggttttg gagacggagt tgtcggtgag tttctggaac gcggcctgga cctccaggtg
     4321 ctggtgggga gtcacgtcga aagcagttac gaagactttt ttgatgtcgg cgggaatccc
     4381 cttgatctgt tgcagagagc ccgaatgggc gatctgggcg aagagctctt tgctgtaaaa
     4441 tccccgggac cgggcgattt cctcgaagaa gggattgacc tcgaagagct gggtgccgga
     4501 cagaacattc cggataaaac tcaccgcaaa gaggggttcg atcccgctgg aacagccggc
     4561 gatgatgctg atcgtgccgg tgggcgcgat ggtgttgacc gtggcattgc gcattcttag
     4621 attcctggaa ggatagacgg acttggggaa gttggggaag actccgcgct cctcggccag
     4681 gacggaggag gccttaaccg attcgcgatg gatgaaacgc atgagcttat ccgcaaaccg
     4741 gacggagtct ttggttccgt agggaatgcc cagacgaatc aggaggtcgg cgaagcccat
     4801 cacccccagg ccgatcttcc ggttggcgaa ggtcatctgc tcgatctggg gaagggggaa
     4861 tttgtttacc tcgattacgt catccaggaa ccgaactccc cagtgaacat actccttcag
     4921 ctttttccag tccactcctc tgcccttcac catacgggcc aggttgatgg aagccagatt
     4981 gcagctttcg tagggcagaa gggggagctc cccgcaggga ttggtcgcct cgatgggacc
     5041 gacctgcggc gtcgggttct tccggttgat ctcatccaga aagatcaaac ccgggtctcc
     5101 ggtcagccat gccgcattga cgatgaggtc aaagatcatc cgggccttca cccggttccc
     5161 ccgttcctga gtgcggggat tgatcaagtc gaagtcccgg ttggccagca ccgccttcat
     5221 gaaaaggtcg gtgaccccca ccgacaagtt gaagttggaa aaacgtttct tctcgatctt
     5281 ggcctcgatg aattcgacga tgtccgggtg gtcgacgcgc aggactccca tgttggcccc
     5341 gcgccgccgc cctccctgga cgatcacctc ggtagcccgg tcgaatatgc tcataaaaga
     5401 gaccggcccg gaagcctccc ctttggtgga ggcaacgatg tcttttttcg ggcggaggcg
     5461 ggaaaaattg aaacccgttc ccccgccggt ctggtggatc ttggccatgc tggtcagggc
     5521 cccgaagatg ccgtcgacgg aatcctccac cggcaggacg aagcaggcgg aaagctggcc
     5581 cagggaagcg ccggcgttca tcagggtggg ggagttgggc ataaattcca gattgcgcat
     5641 catctggtaa aatttttctt cggcctgctc gggggtcacc gaggatgaga agttcttctc
     5701 cccttgagcg acgtgatgcg ccacccgctg gaagagcatg ctcggggttt cgatgatcct
     5761 ccgctgatcg tctttcagga ggtatcttct tttcagcacc tccatggtgt tgaggggtag
     5821 cttcaggtca tccttcatcc ccaggacgga cttggccagg cggatctcac tgcgcctctc
     5881 ccggtacagg atgtaactct tggcgatctg gctatacccc tgattcatca ggaccgtttc
     5941 caccatgtcc tgaatggctt ccacatggag aggcttgtct cggtattttt ctgccaggtt
     6001 ctggacgacg atgtccacca agcgtttggc gatcttcccg gctcgagccg agtcctgaag
     6061 gacttcaaag gctgcccggt agatggcgta ttggatcttc tcaggggtaa agagaacaat
     6121 cgccccatcc cgtttcatca ccttatccgg gattcgcctg gttttcatcc gtttcctttc
     6181 agcgctcgag actcaacctg tttttttccc gctggaaaca tcctgtgggc gcgcgccgcg
     6241 cgcccctaca ttcctaattg agaaaagtgc ctagagtgca ctaaagtccg gagtgcctaa
     6301 agttgaaggc caaagccttt ccttctttta atccgtactg cttatgttcg ccatttcgca
     6361 atccgaattc cgcattctca atcctctcac cccttcggta tgggaaccgt caggcttccc
     6421 tggggcatga gaagaacccg gggcttgggg cccactttgg cgaaagccat ctggagtgct
     6481 tccttcatgt cttttgccgg agtgaaaaga agggtccgag ccaattccgg ctccaggccg
     6541 gagatgagga tgaactgggc cttcttcatc aaccgggtca cggcataggc cttgtggcct
     6601 cccactacga aatgggcccg gatttccgct tcaattctct ccggggtctt gtgttttttc
     6661 atccactcca gatacagatt gtcgcccacc ccttcttcgc actcgcccag gatgatcacc
     6721 acgcccccct cgcgcacggc cagccaggca ttatccatgg tcttctgtag ctgatagaca
     6781 ttgatgtctt tgggatagcc gccgcaggta gcgatcacta gatcggccaa tccgctgatg
     6841 ggcgcgccgt atatggtttc cacgaatttg caggcctcca ggtgggcctg aatgtagtcc
     6901 ccgccgtaga ctttcagaaa ttcctttttc tcgtttagga ctacgttgag aagaaaagag
     6961 ggtctcttca tttcggtccc ctccacctgg tcttcatagg ccggatttcc cttgagcctg
     7021 ccggtgaccg ctcccggctc gagcatgagg gcatggttgc ggcagatggt ttcataccgg
     7081 gtgacccctg gaaacagggc cttccggccg ccgccgaacc cgctgaaaaa atgatataca
     7141 acactgcctg tgcagacgat atgatcggct tccatggccc gcttatggaa ataaacgtca
     7201 tttccccgcg aagtcctccc cgcaaacttg aactgcgagg gatcgtgggc gtcgctgttg
     7261 tacatcttca cccgcctggc agcttctgcc ccggccatct gggccatctc ctcttcggga
     7321 agcatgcggt gggcccccac ggaaaacatg atgaacatgt ccttgtcggg aatccccgcc
     7381 gcgttcaact cgtccagcaa aatgggcatg aaaacatgac tgttggcgat ccgggtcggg
     7441 tcgttcacca ggaaacagac ggtctggccg ggcttaacga tttcccggaa aggcttggat
     7501 tgaatcgggt tgcggatcgc cttttggacc tccgccaccg ggtctttgag tggcgggtag
     7561 tcccgaatcg taagttctcc gatcaccagc cgagggtcca aatcaaagcg gacctctttt
     7621 cttccatact taaacttata ttctttcgtc gccattctcc tcgtccttcc ttcctgcatg
     7681 ctcgggtcca tgaaccttga ttggctcgag tttctatttt attctagcat aattggtgat
     7741 ctcgtacgaa gtctaaaacc gcatcctttc accctggttt ctggggtcca ccatcgattt
     7801 tgacaaaggc cctatgggat gctacaagga atatggagtc tcccgtggga atctattacc
     7861 tggagaaatg gtttcgatca tgtctgacaa agttcagcct tttccctgga tgcccattct
     7921 ttttttaacc gccattttct acctgaattt cacttcgcgt gtcattctgg ctccgcttct
     7981 gccggtgatc gaaaacgacc tcgggatcgg gcacggcgag gcgggttcgc tttttctcta
     8041 cctggcctgc ggttccgggg tgggactggt agggtccggg tttgtttctt cccggctcac
     8101 ccaccggaaa actctctccc tgagcgcaat tatggtggga actgttctat cggttatctc
     8161 cgcttccagt tccctggggc tgatgcgcgc cggtctcgtg tttgcaggaa tctttgcggg
     8221 tctctacatg ccttcggcca tcgccaccct gaccgaagtg gccagccagg aacactgggg
     8281 aaaagccctg gccgtccatg aactgggtcc caatctgggc tttatcaccg ttcccctgct
     8341 ggctgaagcg ctcctgaaat tcttttcctg gcgtgagtct ctcgccctag tgggggtcgc
     8401 ttgcatcgtg gtgggggtcc ttttcctctt ctttggaaag gggggaaaac acaaaggaga
     8461 accccctcgc tttcaatcca tgagcaaaat cgtgaagagc ccttcctact gggtgatgat
     8521 ctccttgttt accgtatcga tcgggtcgag tgtcggactt tattccatga tccca
//
LOCUS       DASPRR010000239        26928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2150064, whole genome shotgun sequence.
ACCESSION   DASPRR010000239 DASPRR010000000
VERSION     DASPRR010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 26928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26928)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26928
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2150064"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VEL68_09235"
     CDS             <1..1393
                     /locus_tag="VEL68_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXZ36189.1"
                     /translation="QAMRGKMAEVVDIIRSDPDVEGVSAYTGTSTTTNTARMFITLKP
                     FAQRKTSASQVIARLRRKLAQVPGAPAYLQAVQDLRIGGRLGNALYQYTLQGDNLSEL
                     NAWAPRMLQRMRTLPQLVDVSSDQQNKGLENTVVIDRSTASRLGITPQVIDDTLYDAF
                     GQRQVSITYTLLNQYHVVMEVEPSFWQRPETLRDIYLPSSNGVLVPLSAFAHFERAAT
                     SLAVNHQSQFPAVTISFNLASGVALGDAVKAVEASVHELGMPGGIRSSFMGVAQAFQA
                     SLANQPFLILAALIAVYIVLGVLYESFMHPITILTTLPSAGVGAVLALLVYRTELSVI
                     ALIGVILLIGIVKKNGIMMVDFALEAERREGKPPVEAIYQACLLRFRPIMMTTMAALL
                     GGLPLALGRGVGSELRRPLGIAIVGGLLLSQLLTLYTTPVMYLYLDRLRLWLKGRRGR
                     PNPSPMIPSSIFR"
     gene            complement(1467..4559)
                     /locus_tag="VEL68_09240"
     CDS             complement(1467..4559)
                     /locus_tag="VEL68_09240"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HXZ36190.1"
                     /translation="MRKGSILITVAVFVSGLGYVLAQEGGQPELQDLTQKKHDVEISQ
                     EPHESGALEPEFEEMRKAGQKTPVRVGPASPSTLRDKLAQGWRFFKKGDYAAAIPLFT
                     QASTAADASTADEAKLGLAYAYLKQGQKGRAKEFFEELAGKDYKTAEVLANLLPILLS
                     DEEYSRAKPYLALLPEKDRRQWEKRFQEAQVRSDYRELAKPPSSGDINRLVRNNREAL
                     ESCLAGDVFAQAAKDLAAAGAKQEAASLYRDLLKCPGADWKARLGWLYGLAEVAPPGE
                     MESLLAEEKKKGPGEYRAEVNTLELSLLRRQLGALPPGSPEAERVSRKILAVRPGDPA
                     AQSALAWTYFNQGKYEEAKRMFSDLHSRYPQEKDYALGLAYTRAKMGKDEEALSLLRA
                     ERERGGLSPEDLKGLNELELAVLRKQLAALPPSSPRIEEVARGILALQPGDLSARRAL
                     AWMYFNQGKDREAYQLFSSLHKENPEDRDYALGLIYTQMRMGKTDEALALAERVGFQD
                     KEWQDIRAQLYLKKAGEAYDQKKYAEAQTYARKALAIQPDNQDARLLSARTYMAQGKP
                     REALPLLLDQYKKSPTPELEKSILSTYEQTGERKKAFQFADSLGKGASPASPKAAAEY
                     YASHDAPVRAAQINPERGTCYYNADKPSIEGSFLLRYKSGSDGFDKLIEADFPITFDY
                     PMFYGKKWSFSLIPAYLDSGSAPSVPYAGNFYRFANGGPKQNDLVTSLWAVKPEVGFQ
                     MEGPVRYGFLLGTSFIGGPVSPLPTFTAGASGKNWLVNVHQLQVEESLLSYAGQKDPY
                     TDKEWGRVLRTGIDGNISFEPWPYYWVFFSAGYDYLWGENVWSNQSVFGNASFGKFLP
                     WGPGDLSAGWFATVKHYQRNTNFFTFGHGGYFSPEVFFITGPTLRYRTTPCATYSIDA
                     QGSVGYMYYRTKDSPHYPLFDENLSALSGSARADATGTYAGESVSRLAFSGKVRGVKL
                     FGNHWLGSAYLGFNNASSYNEWRTGVSLQYFFDSITNAGEFRHFLLREPEDRFQRFSR
                     "
     gene            complement(4622..5662)
                     /locus_tag="VEL68_09245"
     CDS             complement(4622..5662)
                     /locus_tag="VEL68_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HXZ36191.1"
                     /translation="MKALKKILLGLGLLLIWGTAYAQPEREWKAYKDQFLTGDGRVVD
                     FIQGRASHSEGQGYGMLLAAIHDEPQTFSSLWKWTQDNLGMRKGDSLHAWKWGRRPGG
                     QWAVLDYNNATDGDLLIAWALLKGAEKWKNETYRFQAKEIIKSIRENLIVQRRGKLYV
                     LPGYYGFTLKEGEVLNPSYLIFSAFKKFEQTEEPKFWKKVYEDSLEVLKKATFGRLGL
                     PADWVLVDESGASVYEKRSRFFGYEAIRTLLYLAWDENLTVIPGPRGLLDMYKKLGYV
                     PLQVDLVQNNISLEEGPGGYYALYGRAARGLGEAELSRVLLQKASQKVQGEKEDYYSH
                     ILYLLSQIEFKP"
     gene            complement(5649..7952)
                     /locus_tag="VEL68_09250"
     CDS             complement(5649..7952)
                     /locus_tag="VEL68_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HXZ36192.1"
                     /translation="MEKHIFTQAQAEKISRRGMNFLPFLLGAMLIFSGGLFSPSQAQE
                     LTKLKLPLTRLAPVQTTKLQGTFGSYWMKLPIPERWQIEKATLHFSYVNSTALTPRNS
                     RLTVFLNNHPLAQVILQPSSPVGDVAVSLPPRLLKPGYQDVEFNVIQHYTLDCEDPSA
                     PELWTTLELDQSYLEFEFRLKPVPLQLGSIASFLFDSRISGENRVNLVIEELSAEKAE
                     LAGLIASGLAVRFEYRPVFFSVSQTLQPKMDNILIGEAKFVKTLLGERSPEVKGAFLS
                     LMHLPLKEGGPVRRGVSDPGHALIVVSGEKFEQVREAARTLASVSFPYPGSPSMEVKE
                     VRFPAGEESKGKNLLGPGEKYSFETLGFETSTFRGMRPGSRSIELRVPSPFVAPSNEF
                     VWVTLHMAYGSGMRKDSVLNIFFNDKYVSSIFLDNPQGGLFRNYRVPIPAYQLKRGRN
                     ILTFSPVLTPLITGRCDYIQTENLLLTIFGDSSLELPPMTSWVDLPRMELFFQDGFPF
                     TQWPDGRETVYFIAEKSLDTLSAALNLLAIASQKIGYPAYGVRFSFDPPKEANREILV
                     VGPISRVPADLLKTAPIQIGKDGSISYPQLKLPGSEFKGEDSWTRRLLSRFVSPPNLN
                     KSETPVWAAFSRQASSLGPERAILSQFQSPHQMGRSVLLLSANTSQEILRGAVMMWEP
                     VVQGRIEGDVSILELPSPEFQVVSGELGSKYYIGGKGTVSRLTSILYTYPWLFLGLLI
                     LAFIILSYAIYQLLKKQRRRRIGHEGS"
     gene            complement(7964..10366)
                     /locus_tag="VEL68_09255"
     CDS             complement(7964..10366)
                     /locus_tag="VEL68_09255"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ36193.1"
                     /translation="MTDRRSFNFLIYLGIGGSLFFLIYTSSLFSESSLQWFLGWGFLA
                     LLWILKRIPSFQEPPRRVLFLLLAAFITLRYWMFRTFESLLFISFWDSGGMMLLYLAE
                     TYGILIYFLGMFVSIWPLQRDPAPQPANLDSWPTVDVFLPTFNEPPEIVMITAIACTQ
                     LDYPKEKLNIFILDDGSTVERRNAADLEKAKAAWERYNTLRSIAKTLGATYLTREKNS
                     QAKAGNLNEALGIQGLPGDTSLARDRLNWLRETLGPEIGRTVSRGELILMLDCDNVPA
                     RDFLKNTVGYFLRDPKLFLVQTPHFFINPNPVEKNLGTFGSSPGENEMFYSSIQVGMD
                     FWNASFFCGSSALIRRKHLESIGGIQGDTVTEDSETSLALHGQGFHSVYVTRPMACGL
                     SPETFDDFILQRSRWTQGMLQIFSLKNPLGVKGLNLAQRVCYLNCCLFWFFGLARLVF
                     FLSPLLYLYFGVRVYNASEWQVLEYAVPHILGSILTTDYLYGRVRHPFFSELYEAVQS
                     IYLVPVILSTILNPRSPRFKVTPKGKSLEEEYLSPLAGPFYILFFLSIMGIVAAVFRW
                     FEYPLESFAILICFFWNIFNFVFVLLCLGVVWELQQRRRYHRIWIEGQVEVTIPRLGI
                     TVPAALQDVSLGGVSIRPGEAVLPLEDERVIIRAARPGGGSYALPASIKRRLVRKHYS
                     EWGCEFTIPDLAAFSDIVSFAYGDSNRWVRFWEVRKERQARIFRGFWYLIKIGWKGTF
                     RNFKGVLFPHWKRIVRSTRELPGRFALGLSLRRKILKALFGALPGRRPVMENSPKSTS
                     TH"
     gene            complement(10623..10859)
                     /locus_tag="VEL68_09260"
     CDS             complement(10623..10859)
                     /locus_tag="VEL68_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36194.1"
                     /translation="MAVSREQIIRLIAEKVGPSIALEAKLEKGAWRITLTREGKTSLL
                     ELKRGFIEDYLEKGEYQQEMAFEARINKAIKALQ"
     gene            10910..11107
                     /locus_tag="VEL68_09265"
     CDS             10910..11107
                     /locus_tag="VEL68_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36195.1"
                     /translation="MLEPIISSVPYSLLSVINVPLVGNLDKTLLGLAHPQFLKDPSKI
                     RSLERRGDLLGFRAEKFGSSS"
     gene            complement(11336..12028)
                     /locus_tag="VEL68_09270"
     CDS             complement(11336..12028)
                     /locus_tag="VEL68_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXZ36196.1"
                     /translation="MMIAENVARVRERMEAAAKRAGRDLAKVRLVAVSKTVGLDRILQ
                     AIEAGVDSLGENYVQEAQEKIGALEQRISWHFIGHLQSNKAKVAARLFDWVHSVDSLK
                     LAEELSRAGRQQSKILPVLLQVNLGKEETKFGAQEEEVFRLLERMGSLPGVSVKGLMA
                     MPPFFENPEESRPYFRALRILAENVSKRQITGVLMEELSMGMSNDFEVAIEEGATLVR
                     VGTAVFGPRPPR"
     gene            complement(12196..12813)
                     /locus_tag="VEL68_09275"
     CDS             complement(12196..12813)
                     /locus_tag="VEL68_09275"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXZ36197.1"
                     /translation="MHPPILILASASLRRQELLRSAGISFEIIPSEVDEEWGEGETPE
                     DYVVRLARLKAVKVGEKHKTRWVLAADTIVVIDGRILGKPRDRQEAEGMLETLSDREH
                     RVLTGYCLLQISSGKSRQGRVVSRVRFKELSPDEVRWYLDTGEAFDKAGAYAIQGKAA
                     FMVKEVQGSYTNVVGLPLCEVVEALREMGGLSYQPSIPGPKSKIN"
     gene            12877..13278
                     /locus_tag="VEL68_09280"
     CDS             12877..13278
                     /locus_tag="VEL68_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXZ36198.1"
                     /translation="MNHQEISVRTRSRQELLDITEQLSRLVRESKVADGLMVVFVPHT
                     TAAVTINENADPSVQQDILAELNRLVPHTGSYTHSEGNSAAHIQSTLLGPSQTLFIQN
                     GRLALGTWQGVYFCEFDGPRTRKIWVKILSQ"
     gene            complement(13384..15366)
                     /locus_tag="VEL68_09285"
     CDS             complement(13384..15366)
                     /locus_tag="VEL68_09285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXZ36199.1"
                     /translation="MNLPSPGSPFLRDYAKELNPQQLAVVKAEEGPILVIAGAGSGKT
                     RVVTYRVAYLLEAGVHPANILLVTFTNKAAREMLRRVELLMPAALGVSSRIWGGTFHH
                     IGNRILRRHAPLVGFYPNYTILDQEDAKSLMETCINELRLNPKGSHFPRGEVLENMVG
                     LSVNTQRPLEDIVWERFSYFYDLLDDIRAVAGHYQKRKKELNCLDFDDLLFYWKTLLQ
                     DHPEVRARYGEQFHHILVDEYQDTNRIQSDIIDLLAEKHGNLMVVGDDSQSIYSFRGA
                     HFANILSFPDRYPSAKVFKLEINYRSTPEILHLANSSILFNRHQFPKELRTVRPAGAV
                     PCLVPVQDVLEQAYFVGQKIEEIRDGGIPLSEIAVLYRAHYHSMELQMELTRKGIPFQ
                     VRSGLRFFEQAHIKDVCAYLKIICNPMDELGWKRVLQLYPKVGKATAEKIWRLVSSGV
                     PDPWAAVDSREMMPKVPARAREGWRELTRLLVQLRREGMTISPADMIEAILQGGYETI
                     LQAKYPNYESRADDLRQLAVFAGQYASCQDFLSDLALLTSVESEPQSFEEGDGTLTLS
                     SVHQAKGLEWSAVFVIWLAEGRFPTARSLSDSGGEGEEEERRLFYVAITRARDELFLC
                     YPRFAADRGGGETTQRPSRFISELSGEEYERVAPDE"
     gene            complement(15363..16238)
                     /locus_tag="VEL68_09290"
     CDS             complement(15363..16238)
                     /locus_tag="VEL68_09290"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HXZ36200.1"
                     /translation="MISVFSLYLDFFKFLDLVSEDDPWPLYQRLYLQPHEEFLMAYWR
                     NYDHFDLDQIAARVRQIKKADYAQLQDLIQRQNPSILAAEALNRCQAIFPREPQPPVY
                     LFVGFFSADGVTVKVGGAQVIALGLERFRDFQDFPLLVSHEYGHFAWRSLLKDRPAQG
                     PPTLFSKVISEGISVFFSQLVYPEIPLHRHLFLTPERLRWCRENQEALLDLAGADLAA
                     PKLIPILFGPGDPDAGIPPRVGYFLARQMLGHCLAHHGSVILAENFPGSEILFRKILE
                     GGILRRESQEEQAPS"
     gene            complement(16295..18043)
                     /gene="ispH"
                     /locus_tag="VEL68_09295"
     CDS             complement(16295..18043)
                     /gene="ispH"
                     /locus_tag="VEL68_09295"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXZ36201.1"
                     /translation="MARVKIAKNAGFCMGVRRAVDMALVSTRKKSGPIYTYGPLIHNP
                     QVLEILEGKGVKPMQGEDVNQGNRPSKDGGTLIIRAHGISPQERREIKEFGIKVLNAT
                     CPHVGKVQGIINRHAKDHYSIIIAGEKDHAEVKGLLGYAQGRGYVIHTLEELACLPPL
                     GKVCFVAQTTQDETRFQALAEAIQKKYPGAKIFNTICDSTHRRQEEVLALAKKVEAMV
                     VVGGKGSGNTRRLAGISEAAGIPTYHVETEKDLDLQVLSRYSVIGVTAGASTPNWLIL
                     RVVDRLHDLGGRVEPASFLKALGRLVAISYLLLAFGAGCLTYTSILLQGLRFNLSWIF
                     IAALYVFSMHVLNRLTDKASENFNQPGRTDFYERYGKGMITMGIASAGVALILAWFQG
                     ILPFLLLLAISALGMIYNLRLLPGRPRSAFHYEKIKDIPGSKTLLVALAWGVVTSLLP
                     PLAQEGRFLPATPVAFLFTSILVFVRSTLYDFKDIQGDLMVGKETIPIVLGKRKTEGL
                     VVLLLIFLGALLTVAEPMGWATSLASFLLISLGYVVSYYYLYRQKVTWWGFLFEWVVD
                     GSFIFAGLLGLLWSLF"
     gene            18501..20336
                     /locus_tag="VEL68_09300"
     CDS             18501..20336
                     /locus_tag="VEL68_09300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXZ36202.1"
                     /translation="MLVVGSKRKNRSPRSPNIPGHPSVAISCSNEFVRSIEPSSGLKS
                     TVQKISRLVSRFSGEKGECLIHLWNKRLNLLEFAGPHSTGAPWTDPFKPGEGVPGSAF
                     LRKRPVFSTQTPKDAVGTWGEAARAKGIYSILSLPLRGRKSVLGTLTLLSRKSRPGWK
                     AAIPQLVSLTEQAGAAIEDTQSYQKVRDQLKTISRGRKREGEIKKFLENIVDRSADPI
                     MATDLYGKLTFVSRGAEEMFGFPKEQLLGRKISEFYSGGEREARKIMKTLARKDKLRN
                     YETEFIGPGGKKISIILSASILRDARGNPTGTLGVSREITDYKILLNHITRTEQSYQK
                     LFEAVNDAIFFLNREGYFSTFNRMFLKMTGYTEKEIRSFHFSKIIHPEDIPMMVNDHQ
                     KVIRGDYAPQRYTFRLINKEERIIYVEGNFRRLKEKNEVVGVLAVLRDVTEKIRLEKE
                     LLELSITDGLTGLHNQRHFYNELDKEMERAKRQHITLSLLLFDLDDFKVFNDIHGHLE
                     GDKVLRSVAQVVLKSIRRMDSAYRYGGDEFTVILPGATRVEATQVGERIRKTFETTPF
                     LQQIKLSIGLVEFDPQYDLTNFVKYADEAMYTAKKMGGNRIFIRN"
     gene            complement(20667..21170)
                     /locus_tag="VEL68_09305"
     CDS             complement(20667..21170)
                     /locus_tag="VEL68_09305"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXZ36203.1"
                     /translation="MNRAQPKKKRLKGQVGNGWIAQKGFSILELCITLAIIAVVAAIG
                     YPTFQRMAINGNLRSAARQIMGDIALLKERAVAQNTQCSITFEPSNNRYRCSDMDTDQ
                     WKYPTMIARDIQITRAAFGTGRTMVFESRGTVQQAGNIELTNGRGSKATITCNIAGRT
                     YVKFIMR"
     gene            21571..21759
                     /locus_tag="VEL68_09310"
     CDS             21571..21759
                     /locus_tag="VEL68_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36204.1"
                     /translation="MKIGDTKGEQGRFPRFFKVPGNPYTLGHGSFNQGKILEEKQKKE
                     QKKIFKPTRPGQNGLTKR"
     gene            complement(21762..25262)
                     /locus_tag="VEL68_09315"
     CDS             complement(21762..25262)
                     /locus_tag="VEL68_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HXZ36205.1"
                     /translation="MNIRRAFGLSVFVSLICFSLAPVRGQTPTGDEELFSGSVKPNVL
                     IILDNSNSMDEDFFGNAVGSWSTGSKAVEGKRILNDLVTTYANTVRLGLMAYRLPSVT
                     RGSIYNNLHFVSYDPRSYCPTPIDACEEYCRTGRTTARSACQTACSAENPDFLATYID
                     DSITGFSFGSDSRNKYCGLVYPKTNRLPNPADPRGYIYFKQNLPYYDNTPISDSFLFS
                     RDYNTNDYYDDTFQAYGFKTGTSDNPPPSPPADGAGYSSWMFTKHLYPATDSDIALGY
                     NEFGKRVVLLHVGDAWYATTSPGNGYLHVPVDENKTDNRQLEALLAKLQTNEGRPDDY
                     MSCTHTTNPNRCSYLISAGLTPTPGTFQSVITYFQGNYPREERGGRYPTPIQSRAAAC
                     QKNFIVYVTDGLPSVNESGTTGTADSLMPSVIDKIQALRSLAVNVGGTIYSYDIQTYV
                     VGVGLTSEAKAKVDAMAQAGGTAVDGRAYYADNPEQLQEALNAIFTDIQSGTYSFSQP
                     SVSSVRFSDENFIYVASFEPKGGEPFWKGILKKFAVQEDGSLGSMIWEAGSVLEGTTE
                     SLRNIKTLLGGYPEKFTTDLSPLYFDLPWFRTEERDQIVGYVRGKSTLPVGGVAVANP
                     DYPRKLGDIFHSNPVSVGSPSLFFNDPRDRNQAFVAFRSSNQRTSENGKRIIITGAND
                     GQIQAFETGAGREAWSLIAPNLLPKLKDWVHTSHPTTKKHKYFVDGPISAADVWLGTG
                     DGTNKVATDWRTLAVVGLGMGVRGDGDNPDYLWSMSPYCNGPFHDKYNALFRYYCGYY
                     ALDVTNTVNFPSFKWVLDPSGREAPYLGEPWSKMVMSKVKVNGEEKWVGFIGGGYTMG
                     TPPGAKEGKGFFVVDLRTGNVLWSYTNRDNGAMDFIPGTPAIVDRDNDGFIDTAYVGD
                     LSGNLWKFTFCPFDPDGAACGISNWKAMILYAPGGSKLPTFNSPAVAKDQNYYWVFWG
                     TGDKANPNNEGPQNQFLAIRDQDPTSAYTLSNLQNITSTTFTETTRNGWYLNLPGQER
                     VISDATVFNGIVFFTSYTPPDTENLCGATGIGALYGVAMMPLSIGSGTYDPGKGVFSV
                     AGQRRIELGAGISSAPVISLRPVDVKGGTPDVYVAVSGGAGTPAAIKSSSDISSLKDA
                     MAGSEPAPSIIHWRDLRVQP"
     gene            complement(25288..25473)
                     /locus_tag="VEL68_09320"
     CDS             complement(25288..25473)
                     /locus_tag="VEL68_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36206.1"
                     /translation="MRKIEGGVLYTEKNQYSLAGVKVLDLSPRPKDADVKRMPKKTAE
                     MTFLNGQLREVVIRQRQ"
     gene            complement(25672..26130)
                     /locus_tag="VEL68_09325"
     CDS             complement(25672..26130)
                     /locus_tag="VEL68_09325"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HXZ36207.1"
                     /translation="MEKGKESGFALIAALLSLWILTAVGVLVFTVTTQDVRISSRMVG
                     EKKAFLATEAGVHQLAAGFDPLNLSDTAKYNINFQVDAGLDPGTVYRIGTPAIPTRGP
                     ASISMPGYSQSWGSNRFVSTVTGMNNNYQSSVQVAVGVGFGPVEITTAYR"
     gene            complement(26153..>26928)
                     /locus_tag="VEL68_09330"
     CDS             complement(26153..>26928)
                     /locus_tag="VEL68_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXZ36208.1"
                     /translation="MRGKEIGFTLIEVMFAILVGMLLLGAAYVAMTAGQQSSAGVERK
                     VAAQQDVRAALQIMGLDLSMASYNPHYAPNIWHDLPGFGSVVQCTASGDQTFRGVREA
                     TPTAITVEMDIGESNLVGDDQGEIIRYNYNSTGQERLITRETVNCDNMRAASAASPFL
                     GDTIVSGRPRSVRVINRDLGIVNGLGQPAIFRYFDARNPATELYPYSDPPGGDVNQTP
                     RIRRIDITLAVETDEVDPATKARKRMTYSTSVLVRNQAF"
BASE COUNT         6047 a         7545 c         7366 g         5970 t
ORIGIN      
        1 tcaagccatg aggggaaaga tggccgaagt ggtggacatc atccggtccg atcccgatgt
       61 ggagggggtg tcggcttaca cgggaaccag caccacgacc aacaccgccc gcatgtttat
      121 caccctgaaa ccatttgcgc agagaaagac ttccgcctcc caggtgatcg ccaggctgcg
      181 ccggaagctc gcccaggtgc cgggggcgcc ggcttacctt caggcggtcc aggacctgcg
      241 catcggggga aggctgggga atgccctcta ccagtacacg ctccaggggg ataacctatc
      301 ggaactcaac gcctgggccc cgcgcatgct ccagcggatg cgtacattgc cgcaactggt
      361 ggacgtgagc agcgatcagc agaacaaggg gctggagaac acggtggtga tcgaccgcag
      421 cacggcgtcc cgcctgggga tcacgcccca ggtaattgac gatacactgt atgacgcctt
      481 cggccagaga caggtatcga tcacctacac tctcctgaac caataccacg tggtgatgga
      541 ggtggaacca agcttctggc agcggccgga gacgttgcgg gatatttacc tgccctcctc
      601 caacggggtc ctggtcccgc tcagcgcctt cgcccatttc gaaagggcag ccacgtcgct
      661 cgcggtgaac caccagagcc agtttccggc cgtgacgatc tccttcaacc tggcctccgg
      721 ggtggcgctc ggggacgcgg tcaaggcggt ggaagcgtcc gtccacgagc tggggatgcc
      781 cggcggcatc cggagcagct tcatgggggt ggcccaggcc tttcaggcct cgctggccaa
      841 ccagcccttc ctgatcctag cggccttgat tgcggtttac atcgtcctcg gagtcctgta
      901 tgagagcttc atgcatccca tcaccatcct gaccacgctc ccttcggcgg gcgtgggagc
      961 ggtgcttgcc ctcctcgtct atcgcacgga actctcggtc atcgccctga tcggggtcat
     1021 tctgctgatc gggatcgtga agaagaacgg catcatgatg gtggatttcg ccctggaggc
     1081 ggagcgccgc gagggcaaac ctcccgtcga ggcgatctac caggcctgcc tcctgcgttt
     1141 ccggccgatc atgatgacca ccatggcggc cctgctgggc ggccttcccc tggctctcgg
     1201 caggggagtg gggtccgaac tccgccgccc gctgggcatc gccatagtcg gcgggctcct
     1261 tctcagccag ctactcaccc tctacaccac gccggtgatg tacctctacc tggatcgctt
     1321 acggctttgg ctgaagggcc gacggggaag accgaaccca tcccccatga tcccttcttc
     1381 tatattcagg taaggtaggg ccagagcgga gaaaacccct aatccatcct atgaggcttg
     1441 ccaataggga attttcccgg cggatattat ctggaaaatc tctggaacct gtcttcgggc
     1501 tcgcgcagca ggaagtggcg gaattctccc gcgttggtga tggagtcgaa gaagtactgg
     1561 agggagacac cggtgcgcca ctcgttgtag gaggaggcat tgttgaagcc caggtaagcc
     1621 gagccgagcc agtgattccc gaagagtttc accccccgga ccttgccgct gaacgccagg
     1681 cgggagacgc tctctccggc gtaggttccg gtcgcatctg cccttgccga accgctcaag
     1741 gccgaaaggt tctcatcgaa caggggatag tgggggctgt ccttggttcg gtaatacatg
     1801 tagcccaccg atccctgggc gtcgatagag taagtcgcgc agggcgtggt tctatagcgg
     1861 agagtgggac cggttatgaa gaagacctcg gggctgaagt agccgccgtg gccgaaagtg
     1921 aagaagttcg tattccgctg gtagtgcttg accgtggcaa accagccggc agagaggtct
     1981 ccagggcccc agggaaggaa cttgccgaaa gaggcgttgc cgaagacact ttggttagac
     2041 cagacattct ctccccaaag gtagtcatac cccgcggaaa agaagaccca gtagtagggc
     2101 catggctcga aggagatgtt cccatcgatt ccggtacgca gaacccggcc ccattctttg
     2161 tcggtgtagg ggtctttctg cccggcatat gaaagaaggg actcctccac ctggagctgg
     2221 tgaacgttta ccagccagtt tttcccggac gcccccgcag taaaagtagg gaggggagaa
     2281 accggaccgc cgatgaagga agtccccagg agaaacccgt agcggaccgg cccttccatc
     2341 tgaaacccga cctccggctt caccgcccac aacgaagtaa ccaggtcatt ctgctttggc
     2401 cctccattcg caaacctgta aaaatttccc gcgtagggaa cggacggggc gcttcccgaa
     2461 tccaggtagg ccggaatcag ggagaagctc cacttcttgc cgtaaaacat tggatagtcg
     2521 aaggtgatcg ggaaatccgc ctcgatcaac ttgtcgaatc catccgaacc gcttttatat
     2581 cggagcaaga aagaaccctc gatggacggc ttatcggcgt tgtagtagca agtcccccgc
     2641 tccgggttaa tttgcgccgc tcttaccggt gcatcatggg atgcataata ttcggcagca
     2701 gcctttgggg atgcggggga agcccctttt cccagggaat cggcaaactg gaaagctttc
     2761 ttcctctcgc ccgtctgttc ataggtggag agaatggact tttccagttc aggagtaggg
     2821 gacttcttat actggtccaa gaggagcgga agagcttccc gaggtttccc ctgggccatg
     2881 taagtccggg ctgaaaggag ccgggcgtcc tggttatcag gctggatggc cagcgctttt
     2941 ctggcatagg tttgggcttc ggcgtatttt ttctggtcat acgcttcccc ggccttcttc
     3001 aggtagagct gcgcccggat gtcctgccat tccttgtcct ggaaacctac cctttcggcc
     3061 agagccagag cctcatccgt ctttcccatg cgcatctggg tgtaaatcaa tcccagggca
     3121 tagtcccgat cttcgggatt ctccttatgg agtgaagaaa agagctggta ggcctcccgg
     3181 tcctttccct gattgaagta catccaggcc agggctcttc gggcggaaag atccccgggc
     3241 tgaagggcca gaatcccccg ggccacctct tcgatccggg gggaagaggg gggcagtgcc
     3301 gcgagctgtt tccgtagaac ggccagctcc aattcattca gccccttcag gtcttcaggg
     3361 gaaaggcctc ctctttccct ctccgcccgc aagagggaaa gggcctcttc atccttcccc
     3421 atcttcgccc gggtgtaagc cagacccagg gcataatcct tttcttgggg atagcgggaa
     3481 tggaggtcgg agaacattcg cttggcctct tcatatttcc cttggttgaa ataggtccag
     3541 gccagggcgc tctgggccgc ggggtccccc ggtcggacgg ccaggatctt tcggctcact
     3601 ctctcggctt ccggggaacc gggcggcagg gctcccagct gccggcgaag aagggagagt
     3661 tcaagagtat tcacttcggc ccggtactcc cccgggccct tcttcttctc ctccgcaaga
     3721 aggctctcca tttctccggg aggagccact tcggccagcc catagagcca tccaaggcgc
     3781 gccttccagt ccgcgcccgg gcatttcagg aggtcgcggt agagagaagc cgcctcctgc
     3841 ttggctccgg ccgcggccag gtctttggca gcctgggcga agacgtcgcc ggccagacaa
     3901 ctctccagag cctcccggtt atttctcacc agccggttta tatctccgga agaggggggt
     3961 ttggcgagtt ctcgataatc gctccggacc tgagcctcct gaaatctttt ttcccactgc
     4021 ctacggtcct tctccggcag gagggccaga tagggtttgg cccgggaata ttcctcatcg
     4081 gaaagcaaaa tgggaaggag gttggctaag acttctgcgg ttttgtagtc cttccctgcg
     4141 agctcttcga aaaactcttt ggcccggccc ttctgcccct gtttcaagta agcataggcc
     4201 agccccagct tggcctcatc ggctgtggaa gcgtccgctg cggtcgaagc ctgagtgaaa
     4261 agggggatcg ccgcggcgta atcccctttt ttgaaaaacc tccacccctg ggccagtttg
     4321 tccctcagcg tggagggaga tgccgggccg actcgcaccg gggtcttttg gcccgctttt
     4381 ctcatctctt cgaattccgg ttccagagct cccgactcgt ggggctcctg ggaaatctcg
     4441 acgtcgtgct tcttctgggt caggtcctga agctccggct ggcccccttc ctgggccagg
     4501 acgtatccca aaccggaaac gaagacagca acggttatta atatagaacc ctttctcatc
     4561 ttaaaaggcc ttcggccgcc gcaccctcag ccggctcctc gtctatcttc tccttcaacc
     4621 tttatggctt aaattcaatt tgggaaagca agtagaggat gtgggagtaa tagtcctctt
     4681 tctctccctg gaccttctgg gatgctttct ggagaaggac ccggctcagc tccgcctcgc
     4741 caaggccccg agctgccctt ccatagaggg cataataccc tcccggcccc tcttccaggg
     4801 aaatgttgtt ctgaaccagg tctacttgta agggcacgta gccgagtttc ttatacatgt
     4861 ccagcaatcc ccggggaccg gggatcaccg tcaggttctc atcccaggcc aagtagagga
     4921 gagtacggat ggcctcgtat ccgaagaaac ggctcctttt ctcatagacg gaagcacccg
     4981 attcatcaac caggacccag tcggcgggta aaccgagccg gccaaaggta gcctttttca
     5041 gcacctcgag ggagtcctcg tagaccttct tccagaattt gggctcttcc gtctgctcaa
     5101 acttcttgaa ggcggagaag atcaggtacg atggattgag gacttcccct tccttcaggg
     5161 taaagccgta gtatcccggc agcacataca gcttccctcg ccgttggacg atcagattct
     5221 ctcgaatact cttaatgatc tccttggcct ggaaacggta ggtctcattc ttccactttt
     5281 cggccccctt caataaggcc caagcaatga gaaggtcgcc atcagtagca ttattataat
     5341 cgaggaccgc ccactgcccg ccgggccttc ggccccattt ccaggcatgg agagagtcgc
     5401 ccttcctcat gcccagattg tcctgagtcc acttccagag agatgaaaaa gtctgaggtt
     5461 cgtcgtgtat ggccgccagc agcataccgt atccctgtcc ctccgaatgg ctggctcgcc
     5521 cctggatgaa gtcaacaacc cttccatccc cggtgaggaa ctggtccttg taggctttcc
     5581 attccctttc aggctgagcg taggccgtcc cccagatcag gagcaagccc aggcccaaga
     5641 gaattttctt aagagccttc atggccgatt cgtctcctcc tctgcttttt aagcaattga
     5701 tagatggcat aggagaggat gatgaaggcc aggatcagga gcccgaggaa aagccacggg
     5761 taggtataca ggatggaagt cagccgggag accgttccct tgcctccaat ataatatttc
     5821 gatcccaact ccccgctgac cacctggaat tcaggagaag ggagttccag gatggaaaca
     5881 tccccctcga ttcgtccctg gacgaccggt tcccacatca tcaccgcacc ccgaagaatt
     5941 tcctgggaag tattggcgct caaaaggaga acggaccttc ccatctggtg gggggattga
     6001 aattggctga ggatggctct ctccggtccc aggctgcttg cctgacggct gaaagcggcc
     6061 cataccgggg tctcgctctt gttcagattc gggggagaga caaaccggct gaggagcctt
     6121 cgggtccagg aatcctctcc tttgaattct gatccgggca gcttgagctg ggggtaggaa
     6181 atcgaaccgt ccttgccgat ttgaatcggg gccgttttca ggaggtctgc aggaacccgg
     6241 gaaatcggtc ctaccacaag aatctccctg ttcgcttctt tgggtggatc aaaggaaaag
     6301 cgaactccat aggctggata accgattttt tgcgaggcaa tcgccagaag attcaaagcc
     6361 gcggagagtg tgtccagact cttttccgcg atgaagtaga ccgtttccct cccgtccggc
     6421 cattgggtaa agggaaaacc gtcctggaaa aaaagctcca tccggggaag gtctacccag
     6481 ctcgtcatgg gaggcagctc caatgaggaa tcgccaaaga tggtaaggag taaattctcc
     6541 gtctgaatgt agtcgcaccg cccggtaatg agcggggtta gaaccgggct aaaggttaga
     6601 atattccgcc cccttttcaa ctggtaggcg ggaatcggga cccggtaatt ccggaaaagt
     6661 cccccctggg gattgtccag gaaaatggag gagacgtact tatcgttgaa gaagatattc
     6721 aataccgaat ccttgcgcat gccactgccg taggccatat gcagggtgac ccaaacgaac
     6781 tcgttcgatg gggccacaaa aggagaggga acccgcagtt cgatggagcg ggacccgggc
     6841 cgcattccgc ggaaggtact ggtttcgaag cccagggttt caaaagaata tttttccccc
     6901 ggaccgagca ggtttttccc cttcgactct tcccctgccg ggaaacggac ctctttcacc
     6961 tccatggagg gagaaccggg ataggggaac gaaaccgaag ccagggtccg ggcagcctca
     7021 cgcacctgtt cgaatttctc ccccgaaacc acaatcaggg catggcccgg gtcgctgact
     7081 cccctccgga ccggcccccc ttccttgagg ggaagatgca tcaaggatag aaatgctcct
     7141 ttgacctcgg ggctccgctc tcccagaagg gttttcacga acttagcctc cccgatgagg
     7201 atattgtcca tcttgggctg gagggtttgg gagactgaga agaaaaccgg ccggtattcg
     7261 aagcggacgg ccagaccgct ggcgatgagc ccggccagtt cggccttttc cgcggaaagt
     7321 tcttcgatca ccaggttcac ccggttttcc ccgctgatcc gagaatcgaa aagaaagctg
     7381 gcaatggatc ccagttgaag cggcaccggt ttcaggcgaa attcaaattc caggtaagat
     7441 tggtccagtt ccagggtggt ccaaagttct ggggcgctgg ggtcctcgca atccaacgtg
     7501 tagtgctgga tcacattgaa ctcaacatcc tggtacccgg gttttagcag tcggggggga
     7561 agcgagacag ccacatcccc caccggagag gaaggctgga ggattacctg ggccagaggg
     7621 tggttgttca gaaaaactgt gagccgggaa ttccggggag tgagggctgt tgagtttacg
     7681 taggaaaagt gaagcgtagc cttctcgatc tgccagcgtt cgggaatggg aagtttcatc
     7741 cagtaactcc cgaaggtccc ttgcagcttg gtggtctgga ccggagccag ccgggtcagc
     7801 ggcagtttca gcttggtcag ctcctgagcc tgagaaggcg agaaaagccc tccggaaaat
     7861 atcagcatgg cccctaggag aaaggggagg aagttcatcc ctcgccggct aatcttttca
     7921 gcctgggcct gagtgaatat atgtttttcc atatctcccc cgcctaatga gttgaggtcg
     7981 atttcggtga attttccatc accggtcgtc gccctggaag ggcaccgaac agagccttca
     8041 gaatttttct cctcaaagac aagcccaggg caaatctccc cggaagttca cgcgtggaac
     8101 gaacaattct tttccagtgg gggaagagaa cccctttgaa attccggaag gtccccttcc
     8161 agccgatttt tatcagatac cagaaccccc ggaagatccg cgcctgacgt tctttgcgga
     8221 cctcccagaa ccggacccat cgattgctgt ccccgtacgc gaagctaacg atatccgaaa
     8281 aggctgccag gtccggaatg gtaaactcgc atccccactc cgaatagtgt ttccgtacca
     8341 gccggcgttt gatggaagcg ggaagagcgt aagaaccccc gccgggtcga gcggcccgga
     8401 tgatcacccg ctcgtcctct aggggcagaa ccgcctctcc gggccggatg ctgactcctc
     8461 cgagggaaac atcctgtaga gctgccggca cagtgatccc cagacgggga atggttacct
     8521 cgacctggcc ttcgatccag attcggtgat accgtctcct ctgctgcaac tcccagacga
     8581 ctcccaggca gagcaggacg aagacgaaat tgaagatatt ccaaaaaaag cagatcagga
     8641 tggcaaagga ctccagggga tattcgaacc agcggaaaac ggccgccacg atccccatga
     8701 tggacaggaa aaagagaatg tagaagggac ctgccagggg gctgaggtat tcctcttcca
     8761 ggctctttcc tttgggggtg actttgaacc ggggggagcg gggattgaga atcgtcgaca
     8821 gaataaccgg gaccaggtat atgctctgga cggcttcata cagttcggaa aaaaacgggt
     8881 gccgcacgcg cccatacagg taatctgtcg tcaggatcga ccccaggata tggggcacgg
     8941 cgtattccaa aacctgccac tcggaggcat tgtacacccg aactccaaaa taaaggtata
     9001 agagggggga caggaagaag accagacgcg ccagcccaaa aaaccagaac aggcaacaat
     9061 tcagataaca aacccgttgg gctaagttca atcccttcac ccccaagggg ttcttaagag
     9121 aaaagatctg cagcatcccc tgggtccacc ggctccgctg gaggatgaaa tcatcgaagg
     9181 tctccgggga gagcccgcag gccatgggcc gagtcacata cacgctgtga aaaccctgcc
     9241 catgcagggc cagggaggtc tctgaatcct cggtaaccgt atctccttgg atgcccccga
     9301 tgctctccaa atgtttccgc cgaataagcg ccgacgagcc gcagaaaaag gaggcattcc
     9361 agaagtccat tcccacctgg atcgaggagt agaacatttc gttctccccc gggctggacc
     9421 caaaggtccc taaattcttc tccaccgggt tgggattgat aaaaaaatgc ggggtctgca
     9481 ctaaaaaaag tttcgggtcc cgcagaaaat agcctactgt attcttcaga aaatcccgcg
     9541 ccggaacatt atcgcagtcc agcatcagaa tcaattctcc ccggctcacg gtccggccga
     9601 tctccgggcc aagggtctcc ctcaaccagt tcaaccgatc tctggccagg ctcgtgtccc
     9661 cagggagccc ctgaattccc agggcctcgt ttaaattccc cgccttggcc tgggaattct
     9721 tctcccgagt gagataggtc gctcccaagg ttttggcgat ggaacgaagc gtgttgtacc
     9781 gctcccaggc cgctttggcc ttctccaggt ccgctgcgtt ccgtctctcc accgtagacc
     9841 cgtcatccag gatgaagata ttcaactttt ccttggggta atccagttgg gtgcaggcga
     9901 tcgccgtaat catgacgatc tcggggggtt cattgaaggt ggggaggaag acatccacgg
     9961 ttggccacga atccaaattt gcgggctggg gagccggatc gcgttgcagc ggccatatag
    10021 aaacaaacat tcccaaaaaa taaatcagga tcccgtatgt ctcggccagg tacagaagca
    10081 tcatgccccc gctgtcccag aagctgatga agaggaggct ctcaaaggtc cggaacatcc
    10141 agtaacggag ggtgatgaaa gcggccagga gaaggaagag gaccctccgc ggaggttcct
    10201 ggaaggaagg gattcttttc agaatccata ggagggccaa aaatccccag cccaagaacc
    10261 attgtaagga ggattcagaa aagagactgc tggtgtaaat gaggaagaaa aggcttcccc
    10321 caattcccag atagatcagg aagttgaatg atcttctatc cgtcaaaagg cccctccctc
    10381 acaatttatc agactaagga aattgcaaga ttcgtaccga atttatcctc ctatcttccc
    10441 caggtgaatg ccttcctcaa gctcccaacc gcaccccctg ggaaaagggt gttcgcaaat
    10501 cagcaaatgg cggggggatt acgtcgatgg ataaggctaa atttcaatga aacaaataag
    10561 cagggttttg tgaagaaggg gtccttccga ttttttttcg aagggaaagg ccagagaacc
    10621 ttttattgta aggctttgat cgctttattg atgcgcgcct cgaaagccat ctcctgttgg
    10681 tactctccct tttccaagta gtcttcgata aacccccgct tcagttccaa gaggctggtt
    10741 tttccttccc tggtcagggt gatccgccag gccccctttt ccaacttcgc ttccaaggcg
    10801 atcgagggcc ccactttctc cgcgatcaga cggataatct gttctctact gacggccata
    10861 attccccccc cgaagaaacg ggaacgagga aacagggaga cccgacagaa tgctggaacc
    10921 catcatttca tccgtcccct attcccttct ttccgtaatt aatgtaccat tggtcggaaa
    10981 tttggacaag acccttcttg ggcttgccca cccccagttc ttaaaagatc cttcgaaaat
    11041 aagatctctg gaaaggaggg gggatctctt ggggttcagg gcggagaaat ttggatcatc
    11101 atcctgaggc ggtggttctt tcatgggatc gggcctgtcc tggggggatt gaaacggaaa
    11161 catgctttgg ttctctattc tgcttggagg aatctcctct caggcctctc cctgcggaac
    11221 agggggaggg gtttcatgga aggataagca cccgccgagc ctgatgatcc aaattcctcc
    11281 accctgaacc ccaaaaagca tgaaaaactg ggggtgggca agccttcttg ggaggttacc
    11341 ggggggggcg ggggccgaaa acggccgtcc ccacgcgaac cagggtagct ccttcctcga
    11401 tggctacttc aaagtcgttg gacattccca tggataactc ctccatcagt acaccggtaa
    11461 tctgcctttt ggacacgttc tccgccagga tgcgaagggc ccggaagtat ggccgggact
    11521 cctccgggtt ttcgaagaaa ggcggcatgg ccatcagccc tttgaccgag acccccggca
    11581 aagaacccat ccgttccaga agtcggaaga cttcttcctc ctgagctccg aacttggtct
    11641 cttctttccc cagattgacc tggagcagga ccgggaggat tttactctgc tgcctccccg
    11701 cacggcttaa ctcctccgcc agcttcaagc tatccaccga atggacccag tcgaagagac
    11761 gggccgccac ttttgctttg ttgctctgca ggtggccgat gaaatgccag ctgatcctct
    11821 gttccagcgc cccgattttc tcctgggcct cctggacata attttcaccc agagagtcca
    11881 cccccgcctc aatggcctgt aggatccgat ccagacccac cgttttgctt accgccacca
    11941 accgaacttt ggcgaggtcc cgcccggccc gtttggcagc ggcctccatt cgttcccgaa
    12001 ccctggctac attttcggcg atcatcatct cgcgactacc tccgacggag ggatgacctc
    12061 ccccaaaaag ctaatgaatt tttccccccg ttcttaagaa aacatcctta cggctaagca
    12121 gaaaaattcc aggtcccccc ctgatcctcc atacgccggt gctcgaaagc gactgggttg
    12181 aaatttccaa atttttcaat ttatttttga tttggggccc gggattgagg gttgatagga
    12241 aagccccccc atttcccgca gcgcttcgac gacctcgcaa aggggaaggc ccaccacatt
    12301 ggtgtatgat ccttggactt ctttgaccat gaaggctgcc tttccctgga tggcgtaggc
    12361 ccccgctttg tcaaaggcct ctccggtatc cagataccag cggacctcat cgggggaaag
    12421 ttctttgaac cggactcggc tgaccactct cccttgccgg ctctttccgg aggaaatctg
    12481 gagaaggcaa tagccggtga gcacccgatg ctcccggtcg ctcagcgttt ccagcatccc
    12541 ttccgcttcc tgccggtccc tcggcttgcc cagaatcctc ccatcgatga ccacgatcgt
    12601 gtccgccgcc aggacccatc gggtcttgtg cttctcgccc acctttaccg ctttcaatcg
    12661 ggccagacgg acgacgtaat cttccggtgt ttctccttct ccccattcct cgtccacctc
    12721 gctcggaatg atttcaaagg agatacccgc cgaacgcaga agctcttgcc gccgcaatga
    12781 agctgaggct agaattaaga tgggtggatg catgactgcc ctctaggtaa gggggttcat
    12841 tattgaaaaa agcacaggct taaaaaggag ccggaaatga atcatcagga aatcagcgtc
    12901 cgaacccgga gccggcagga actcctggat atcaccgagc agctttcccg tttggtccgg
    12961 gagagcaagg tggccgacgg actcatggtg gtctttgtcc ctcataccac cgccgcggtc
    13021 accatcaatg aaaacgccga ccccagcgtg cagcaggata tcctggccga gctgaaccgc
    13081 ctcgttcccc acacaggctc atatactcat tcggaaggga actcggccgc ccacattcaa
    13141 tccaccttgt tggggccctc ccagaccctc ttcattcaaa atggacggct ggccctggga
    13201 acctggcaag gggtttattt ctgtgaattc gacggccctc gaacccgaaa aatctgggtg
    13261 aagatcctct ctcaataaga gatcaatgat caatggccaa tgatcactgg ttaatggtca
    13321 atgattaatt gttaaatggc cttttccatt gcccattgct tattgctcat tggccattgg
    13381 tccttattca tccggggcca cccgttcata ttcctctcct gaaagctcgg agatgaaccg
    13441 ggaggggcgc tgggttgtct ctcctccgcc ccggtcggcg gcgaaccgag gataacaaag
    13501 gaaaagttcg tcccgggcgc gggtgatcgc tacgtagaaa agtcttcgct cttcttcctc
    13561 cccttccccg cctgaatcag aaaggctccg agccgtggga aaccggcctt cggccagcca
    13621 gatgacaaac acggccgacc attccagacc tttggcttgg tgaaccgagc tcaaggtcag
    13681 cgttccatct ccttcctcaa aggactgggg ttcactttca accgaggtca ggagagcgag
    13741 atcgctgaga aaatcttggc aggaagcata ctgcccggca aaaacggcca gctggcgaag
    13801 gtcatccgcc cgggattcat agtttggata cttggcctga agaatcgttt cgtatccccc
    13861 ctgtaaaatg gcttcgatca tgtctgcggg gctgatcgtc attccctcgc ggcgcaactg
    13921 gacgagcagc cgggtgagtt cacgccagcc ctcccgcgct ctcgccggaa cctttggcat
    13981 catctctctg gaatccaccg ccgcccaggg gtccgggaca ccggaggaga ccagccgcca
    14041 gattttttcc gccgtggctt tccccacttt ggggtagagc tggagcaccc gcttccagcc
    14101 cagctcatcc atagggttgc agatgatttt caggtaggcg catacatcct tgatgtgggc
    14161 ctgctcgaag aagcgcaacc cggagcggac ctggaaaggg atccccttgc gggtcagttc
    14221 catctgcaat tccatggaat ggtagtgggc ccggtacaag acggcgattt ccgatagggg
    14281 gatccctcca tcccggattt cctcgatttt ctggcccacg aaataggcct gttccaaaac
    14341 gtcctggacg ggaaccaggc aagggacggc gcccgcgggc cggaccgtcc ttagttcttt
    14401 cgggaattga tggcgattga agaggatgct ggaattggcc aggtggagga tctccggggt
    14461 cgaccgatag ttgatctcca gcttgaaaac cttggccgaa ggatatcgat cgggaaagga
    14521 gagaatgttg gcaaaatgag cccctcggaa ggagtagatg gactgagagt cgtcccccac
    14581 gaccatgagg tttccatgtt tctcagccaa caggtcgatg atgtctgatt ggatacggtt
    14641 ggtgtcttga tactcatcca ccaggatgtg gtggaactgt tctccatatc gggctcgcac
    14701 ttcgggatga tcctgaagca gagttttcca gtagaagagg agatcgtcaa aatccaggca
    14761 attgagttcc ttttttcgct tctggtagtg cccggccacc gcccgaatgt cgtccagcag
    14821 atcgtagaaa taggaaaaac gctcccatac tatgtcttcc agcggccgct gggtatttac
    14881 ggaaagcccg accatgttct ccaaaacctc tccccggggg aaatgggagc ccttgggatt
    14941 cagccgaagt tcgttgatac aggtctccat gagactcttg gcgtcctcct ggtccaggat
    15001 ggtgtaattc gggtagaaac caacgagcgg ggcgtgacgg cggaggatac ggtttccgat
    15061 gtgatgaaaa gtcccccccc aaatccggct gctgacccct aaggccgccg gcatgagaag
    15121 ttcgacccgc cggagcatct cccgggcggc cttgttggtg aaggtgacca aaaggatgtt
    15181 cgctgggtgg accccggctt cgagaagata ggccactcgg taggtcacca cccgagtttt
    15241 gccgcttcct gctccggcga tgactaagat ggggccttct tccgccttga cgactgccag
    15301 ctgctggggg ttgagttcct tggcataatc ccgcaaaaag ggagagccgg gggatgggag
    15361 attcatgagg gggcctgctc ttcctggctt tcccttctta ggattccgcc ctccagaatt
    15421 tttcggaaca gaatttcaga cccgggaaag ttctcagcca agatgacgga gccatggtgg
    15481 gcaagacaat ggcctagcat ctgccgggca agaaaatagc ccacccgggg aggaattccg
    15541 gcgtcggggt ctccaggacc gaagagaatg ggaatcagtt ttggggcagc caggtccgcc
    15601 ccggccaaat ccaaaagggc ttcctgattt tcccgacacc accggagccg ttcgggggtc
    15661 agaaacagat ggcggtgcag gggaatttct ggataaacca gctgcgagaa gaagactgaa
    15721 attccttcgg agatcacctt ggaaaagagc gttggcgggc cttgggcagg acgatctttc
    15781 aggaggcttc gccaggcgaa atggccgtat tcatgggaaa ccaggagggg gaagtcctgg
    15841 aaatcccgaa accgctccaa tcccagggcg attacctgag ctccgcccac cttcacggtg
    15901 actccatcgg cgctgaaaaa tccgacgaaa agatagactg ggggctgggg ttcccggggg
    15961 aagatcgcct ggcatcgatt cagtgcctct gcggccagaa tggacgggtt ctgcctctga
    16021 ataaggtcct ggagctgagc gtaatccgct ttcttgattt gcctgactct ggcggcgatt
    16081 tgatccaagt cgaaatggtc gtaattcctc cagtaagcca tcaggaattc ttcatggggc
    16141 tgcaggtaga ggcgctggta taagggccag gggtcgtctt ctgacaccag atcgagaaat
    16201 ttaaaaaaat cgaggtacag ggaaaagaca gaaatcataa aaccccgggt aagactcaaa
    16261 tttcttaaga gcccgaagcc caaactcctt caatctaaaa caacgaccag agaagaccta
    16321 gaagcccggc aaagatgaag ctcccgtcca cgacccactc aaagagaaat ccccaccatg
    16381 taaccttctg ccggtagaga taataataag aaaccacgta tccgagtgag atcaaaagga
    16441 aggaggccaa agaggtggcc catcccatgg gttcggctac ggtcagaagc gctcccaaga
    16501 agatcaaaag gagaaccacc aaaccctctg tcttccgttt ccccaatacg atggggatgg
    16561 tctctttccc caccatgagg tccccctgga tgtctttgaa atcgtagaga gtggaccgaa
    16621 cgaagaccag aatcgaggtg aaaagaaaag cgaccggcgt ggcgggaagg aaacgtcctt
    16681 cctgtgccag gggggggaga agcgaagtca cgactcccca ggccagggct accaggaggg
    16741 ttttggaccc ggggatatct ttaatcttct cgtagtgaaa agcactcctg ggccgacctg
    16801 ggaggaggcg caggttgtat atcattccca acgcggaaat agccaggagc aatagaaaag
    16861 ggagaattcc ttgaaaccaa gccaggatga gcgccacccc ggcggaggcg attcccatgg
    16921 tgatcatccc ttttccgtac ctctcgtaga aatccgttcg tcccggttgg ttgaaattct
    16981 ccgaggcctt gtcggtcagg cggtttagta catgcatgga aaagacatac agggcagcga
    17041 tgaaaatcca agagaggttg aaccgcaatc cttggagcag gatgctggtg taagtcaagc
    17101 agcccgcccc aaatgccagg aggagatagc tgatcgccac cagcctgccc aaggctttga
    17161 ggaaggaagc cggctccact cggcccccca ggtcatggag gcgatctacg acgcgcagaa
    17221 tcaaccagtt gggagtggaa gctccggcgg tgactccaat gactgagtag cgggataaga
    17281 cttgcaggtc caggtccttt tctgtctcca catgataggt gggaatgccg gccgcttcgg
    17341 aaattccggc caaccgacgg gtgttcccgc tgcctttccc ccccaccacc accatggctt
    17401 ccactttctt ggccagagcc agcacctctt cctgccgtcg gtgggtggag tcgcagatgg
    17461 tgttgaagat tttcgccccg gggtattttt tttgaatggc ctccgctagg gcctgaaagc
    17521 gtgtttcgtc ttgggtggtc tgggccacga agcagacttt tcccagggga gggaggcagg
    17581 ctagctcttc cagggtatgg atcacgtatc cccgtccttg agcataaccc agcagacctt
    17641 tgacctctgc gtgatccttc tctccggcaa tgataatgga gtagtgatct ttggcatggc
    17701 ggttgatgat cccttggact tttcccacgt gggggcaggt ggcattcagg accttgatcc
    17761 caaactcctt gatctctcgc cgctcctggg ggctgatccc atgcgcccgt atgattaagg
    17821 ttcccccgtc cttgctcggc cgattcccct ggttgacgtc ctctccttgc atgggcttaa
    17881 ctccctttcc ctccaggatt tctagaacct gaggattgtg gattaacggc ccgtaagtat
    17941 agatgggccc gcttttcttg cgggttgaaa ccagggccat atccaccgcg cggcggactc
    18001 ccatgcagaa accggcattt ttggctattt ttacgcgggc catggaccaa agaaagtcga
    18061 ttgacctgga atagaaaacg tttttaggct acctttattt gagattatcg gaaatgttga
    18121 ggagtgtcaa tgaggccggt ctcggctttt cctttaaagg acaagcaaga tgcctccacg
    18181 actgtatgat cctttcctgc ccgaacgctt ggccgaagca agagggttct ccgttgacaa
    18241 aaaaagggcg ttatctagtc gttaaagcta tagaattgaa aaggccctta aaatgattta
    18301 agaaatgcaa ttaaaaagtt gacaaaaaat ggctgaagtg taatattaac ggcattaacg
    18361 gtgttgaata gggatcgtct agaagggaga agagggtttt tagaacgaat cttcaggggc
    18421 atttaaaggc caacggctgg gaggtaaatt aactagaacg gattcccgag attctttttt
    18481 ctcttctaag gtttcaaaga atgctggtcg tcggatcaaa aaggaaaaac cgatctcccc
    18541 gatcgccgaa tatccctggc catccttccg ttgcgatttc ctgctccaat gaattcgtcc
    18601 gctccatcga acccagttca ggcctgaaga gcacggttca aaaaatctcc cggctggttt
    18661 cccgattctc cggagaaaag ggagaatgcc tgattcatct ctggaataaa aggctaaacc
    18721 ttctggaatt cgccgggccc cacagcacgg gagctccctg gaccgacccc ttcaaacccg
    18781 gcgagggggt ccctggctcc gcttttctcc ggaaaagacc ggttttctcg acccagactc
    18841 caaaagacgc tgttggaacc tggggggaag cggcccgagc aaaggggatt tactctattc
    18901 tctctctgcc cctgcgggga agaaagtccg tattggggac ccttaccctt ttgagtcgaa
    18961 aatcgcggcc cggttggaaa gccgccatac ctcagctcgt gtcgctgact gagcaggccg
    19021 gtgcggccat cgaagacact caatcgtacc agaaggtccg ggatcagtta aaaaccatct
    19081 cccgggggag aaaaagggaa ggagagatca aaaaattcct ggaaaacatc gtggatcgct
    19141 cggctgaccc catcatggcc acagacctct acggaaaact aacctttgtg agccgggggg
    19201 ctgaggaaat gttcgggttt cccaaggagc aacttctcgg aagaaagatt tccgagttct
    19261 attccggcgg agaacgggaa gccagaaaga tcatgaaaac cctggcgcgg aaggataagc
    19321 tgcgcaacta cgagaccgaa ttcattggac ctgggggcaa gaaaatttcc atcattctgt
    19381 ccgcttccat cctccgggat gcccggggaa acccaaccgg gacgctgggt gtcagccggg
    19441 agattaccga ttacaaaatc ctcctcaacc atatcacccg gaccgagcag agttaccaaa
    19501 agttgttcga ggcggtgaat gatgccatct tcttcctgaa ccgcgaggga tacttcagca
    19561 ccttcaaccg catgtttctg aagatgaccg gttatacgga aaaagaaatt cggagtttcc
    19621 atttttccaa aatcatccat cccgaagaca tccccatgat ggtcaatgat caccaaaagg
    19681 tcatccgggg ggactatgct ccacaacgat acaccttccg attgatcaat aaggaagaaa
    19741 ggatcatcta cgtggagggg aacttccgcc ggctgaagga aaagaatgag gtggtgggag
    19801 ttctggcggt gctgcgcgat gtgaccgaaa agatccgcct ggagaaagag ctgctggagc
    19861 tttccatcac cgacgggttg accggcctcc acaaccagag acatttctac aatgagctgg
    19921 acaaggagat ggagcgggcc aagcggcaac acatcaccct gagcctgctc ctcttcgacc
    19981 tggacgactt caaggttttc aacgacatcc acggccacct ggaaggggat aaggtgttgc
    20041 ggagcgtggc ccaagtggtt ttgaagtcca ttcgccggat ggattcggct taccgttatg
    20101 gaggggacga gttcaccgtc atcctacccg gggccacccg agtcgaagcc acccaggtgg
    20161 gcgaacgaat ccgcaaaact ttcgaaacca cgccttttct acaacagatc aagctctcca
    20221 ttggactggt ggagttcgac ccccagtacg acctgaccaa tttcgtcaag tacgccgacg
    20281 aagcgatgta tactgccaaa aagatggggg gaaatcgaat ctttattcgc aattagaaga
    20341 ccgggtttgc ttcgtccgac tgggctcccc gccttttcac ccagaaatcc agccccttct
    20401 ttcctctgag gattttcacc atattgaaca tcgcaagaag atgctcaaag aggaaacact
    20461 gacctttaac ctggtccccg tgtcttgctc cggcggaact taccgaagca agcttcgagc
    20521 gcttaatgag gattgcactc cggctcactc aggtagtgga tagtcccgga aagctcctcc
    20581 ctgaaggaag cagggtggaa aaggttctgc tccccgccct gatcaggaaa agggagggtt
    20641 gcaccttgac ccttcagact cccttcttag cgcattataa atttgacgta cgttcttccg
    20701 gcaatgttgc aggtgatggt agccttcgaa cctcgaccgt tggtcagttc gatattcccg
    20761 gcctgttgaa cggtcccccg gctttcaaag accatggtcc tgcccgtccc aaaagccgct
    20821 ctggtgatct ggatgtcccg cgcgatcatg gtggggtatt tccattgatc cgtatccata
    20881 tccgaacagc ggtagcggtt gttcgagggt tcgaaggtta tggaacactg ggtattctgg
    20941 gccacggctc tttctttcag gagagcgatg tcgcccatga tttgccgggc ggcactccga
    21001 aggtttccgt tgatggccat tctctgaaaa gttggataac caatagcggc gacaacggca
    21061 ataatggcca gggtgatgca aagctccagg atcgagaatc ccttttgagc tatccatcca
    21121 ttccccacct gcccttttaa cctttttttc tttggctggg cccggttcaa ttcctgtcct
    21181 caatgattga tatcattaag tcatcaaagc aataatcatt cctcgattca ggtattttta
    21241 tttttcgctg aaaccgaccc cggttgacta taatattcgt taataatccg caacttaaca
    21301 attattctgg attttttgat tcttctttca attatgggag agagcaagct tttcagacat
    21361 cctgttggtc gttttaaaaa ttttttaagt aatctttaag atttttaatg gctcaaagga
    21421 gggtctatgg gtgggcctca aggctgtcac aaagattttt cttgttcttt ttgaggggga
    21481 gagattgtag cagaggtcta ggcgagaaag gaacttttcc gagcagctta ccagtcagga
    21541 atgaattttc aggaaaccct tagcatctgg atgaaaatcg gggataccaa aggggagcag
    21601 gggcgatttc ctcgattctt taaggttcct ggcaatccct acacccttgg ccatggctca
    21661 tttaaccaag ggaagattct tgaggaaaaa caaaaaaaag agcagaaaaa aatcttcaaa
    21721 ccaacccgtc ctgggcaaaa tggtcttaca aaacgatgaa cctagggctg gacacggagg
    21781 tctcgccagt ggatgatgct cggggcaggt tcggacccgg ccatggcatc tttgagcgag
    21841 gaaatatcgg agctagactt gattgctgcc ggcgtccctg cccctccgct caccgccaca
    21901 tagacatccg gcgttccccc ttttacgtca acgggcctca gggagatgac cggggccgaa
    21961 gaaatcccgg ctcctagctc aatccttctc tggcccgcta cagagaatac acccttgccg
    22021 gggtcgtagg ttcctgaacc gatggaaagg ggcatcatgg caactccgta aagcgccccg
    22081 attcctgtgg ccccacagag gttttcggtg tcaggcggag tatagctggt gaaaaagacg
    22141 atcccattga aaaccgtggc atcggagatc accctttcct gcccgggtaa attgaggtac
    22201 cagccgttcc ttgtcgtctc ggtgaaagtt gtgctggtaa tattctgaag attgctcagg
    22261 gtataagcgg aagtggggtc ctgatcccga atggccagga attgattttg cgggccctcg
    22321 ttgttgggat tcgccttgtc tccggtgccc cagaaaaccc agtagtaatt ctggtccttg
    22381 gccaccgctg gagagttgaa ggtagggagc ttgcttcccc ctggggcgta tagaatcata
    22441 gccttccaat tgctgatacc gcacgccgct ccgtccgggt cgaagggaca gaaggtaaac
    22501 ttccagaggt ttccggatag gtcgccgaca taggcagtat cgatgaaccc gtcattgtcc
    22561 cgatcgacaa tggccggggt gcccgggatg aagtccatgg ctccattgtc tctattggta
    22621 tagctccaga gaacatttcc ggtccgcagg tccaccacaa aaaacccttt tccctccttc
    22681 gccccaggcg gagttcccat ggtgtaaccc ccgccgatga aaccgaccca tttttcctcg
    22741 ccgttaacct taaccttgct catgaccatc ttgctccagg gttcacccaa gtaaggcgct
    22801 tccctgccgc ttgggtccag gacccacttg aagcttggaa aatttacggt attggtcacg
    22861 tccagagcat aataaccgca gtaatagcga aaaagcgcat tatatttgtc atggaacgga
    22921 ccattgcaat agggtgacat gctccagaga taatcgggat tgtctccatc accccggact
    22981 cccataccca ggccgaccac cgcgagagtc ctccagtcgg tggctacctt gttggtcccg
    23041 tctcccgtgc caagccagac atctgccgcg ctgatcggcc catccacaaa atacttatgc
    23101 ttttttgtag tggggtgaga agtgtggacc caatctttca atttgggcaa caggttcggg
    23161 gcaataaggc tccatgcttc tctccccgct cccgtctcaa aggcttgaat ttgtccgtca
    23221 ttggcaccgg tgatgatgat ccgttttcca ttttccgaag tccgctggtt gctactccgg
    23281 aatgctacga aagcctgatt tctgtcgcgc gggtcgttaa aaaagagaga gggggacccg
    23341 acgctcacag gattggaatg gaagatgtcg cccagtttgc gcggatagtc cgggttggcc
    23401 actgccaccc ctcctaccgg cagggtggat ttgccccgca cgtaccctac gatctggtcc
    23461 cgttcttctg tcctgaacca ggggaggtcg aaatagaggg gggagaggtc agtggtgaac
    23521 ttctccggat aacccccgag caaggtcttt atgtttcgca agctctccgt cgtgccttcc
    23581 agcaccgagc cagcttccca gatcatgctg cccaggctcc catcttcctg aacggcgaac
    23641 ttcttcaaga ttcctttcca gaagggttcc ccccccttgg gctcaaaaga ggcgacatag
    23701 atgaaattct cgtccgaaaa gcgaactgag gaaaccgagg gctgggagaa ggagtaggtg
    23761 ccggactgga tatcggtaaa gatcgcattc agggcttctt gcagttgctc gggattgtcg
    23821 gcatagtagg cccggccatc cacagccgtt cccccggcct gggccatggc atccactttg
    23881 gccttggctt cgctggtcaa tcctacaccc acaacatagg tttgtatgtc gtagctgtat
    23941 atggttcccc ccacgttgac tgcgaggctt cgcagggctt gaatcttgtc gatgaccgag
    24001 ggcatcaagg agtcagccgt tcccgttgtc ccgctttcat tcacgctggg caacccgtcg
    24061 gtcacataga cgatgaagtt tttctgacag gcggccgccc gcgattgaat cggcgtgggg
    24121 tacctgccgc ccctctcttc cctggggtaa ttcccctgga aataggtaat aacggactgg
    24181 aaagtgccgg gggtcggagt gagccctgca ctgataaggt aggagcacct gtttgggttg
    24241 gtagtgtgcg tgcagctcat gtaatcgtcc ggtcttcctt cattggtctg caatttggcc
    24301 agcagagcct ccagttgccg gttgtcggtt ttgttctcat ctaccggaac atgaagataa
    24361 ccgtttcccg gtgaggttgt ggcgtaccag gcgtctccca catggagaag gaccacccgc
    24421 tttccgaact cgttgtaacc cagggcgatg tccgaatccg ttgctgggta gaggtgcttt
    24481 gtgaacatcc aggaagaata ccccgccccg tccgccgggg gggagggagg aggattgtct
    24541 gaagtcccgg ttttgaaccc gtatgcctgg aaagtatcgt cataataatc attggtattg
    24601 taatctcgag agaagagaaa actatccgat ataggggtgt tgtcataata aggaaggttc
    24661 tgcttgaaat agatataccc tctcgggtcc gcagggttgg gaaggcgatt cgttttcggg
    24721 taaaccaagc cgcagtattt attcctcgag tccgacccaa aactgaagcc ggtgatgctg
    24781 tcatcgatgt acgtcgccaa gaaatccggg ttttctgcgc tgcaggctgt ctggcaggcg
    24841 gacctggctg tagtcctgcc ggtgcggcag tattcctcgc aagcatcgat gggggtaggg
    24901 caataggacc tcggatcgta ggacacaaaa tgaaggttgt tataaataga ccccctggtc
    24961 accgacggaa gccggtaggc catgagtcct aagcgtacgg tgttggcata ggtggtgacc
    25021 aggtcgttga ggatccgctt accctccacc gctttgctgc ccgtgctcca ggagcccacg
    25081 gcgttgccga aaaaatcctc gtccatgcta ttggagttgt ccaggatgat gaggacgttg
    25141 ggcttgactg atccgctaaa gagttcctca tcccccgtag gtgtctgccc gcggacggga
    25201 gctaaggaga aacagatcaa gctgacgaag acggataaac cgaacgccct tcttatattc
    25261 atattccttc cctctgtctt tctggtatta ctgccgctgg cgaatgacca cttcccggag
    25321 ctgcccgttc aggaaggtca tctccgcggt ctttttgggc atcctcttca catcggcgtc
    25381 cttaggcctc ggactcaagt ccaagacctt taccccggcc aggctgtact ggttcttctc
    25441 ggtgtacaga actccccctt cgatttttct caccaactcc gtaatccggc gtaccgagct
    25501 cttggcagtc gatgttttat tctcctctgg cgacttcctg accttttcac cctgaatccc
    25561 ttcgtttccg agggaagggc cggggatagc cgtccatgct tcagagctta agcatgtgac
    25621 tgctaagagg accagtaaaa attttaaaaa aaccatgagg ggactccttt attatcggta
    25681 ggcagtagtg atctccaccg gaccaaagcc cacccccact gccacttgga ccgagctctg
    25741 gtagttgtta ttcatccccg ttaccgttga cacgaaacga ttcgatcccc aggactggga
    25801 atacccgggc atggaaatcg aggccgggcc tcgggtgggg atggccggcg tcccgatccg
    25861 atataccgtg cctggatcca aacccgcgtc cacctgaaaa tttatgttgt acttcgccgt
    25921 atcgctcaaa ttcaaaggat cgaaacccgc agcgagctgg tgaaccccgg cttcagtggc
    25981 caaaaaggcc ttcttttccc ccaccatacg ggagctgatg cgcacgtcct gggtggtcac
    26041 ggtgaagacc agaaccccta cggcggttaa aatccataac gacaggagcg cggcgatgag
    26101 cgcaaatcct gactctttcc ctttttccat ccctcgactc tctttctttg ggtcaaaacg
    26161 cctggttcct caccagcacg ctggtggaat aggtcatccg tttcctggcc ttggtggccg
    26221 ggtccacttc atccgtctcc accgccaggg tgatgtcgat tcggcggatt cggggcgtct
    26281 ggttaacgtc tccgccgggc ggatccgaat aagggtaaag ctcggtagcg ggattccgcg
    26341 catcgaagta acggaagatc gccggttgac cgaggccgtt cacaatcccc aaatcccggt
    26401 tgatgacccg cacggacctg ggacggccgg agacgatggt atcccctaga aaaggggatg
    26461 ccgcgctcgc ggctcgcatg ttgtcacaat tgaccgtctc ccgggtgatt aaccgttcct
    26521 gcccggtaga gttgtagttg taccgaatga tctctccctg gtcgtccccc accagattgc
    26581 tttccccgat gtccatctcc actgtgatgg ccgtgggggt cgcctcccgg acccccctga
    26641 aggtctgatc tcccgacgca gtgcattgca ccacggagcc aaatccagga aggtcgtgcc
    26701 agatattggg ggcgtaatga ggattgtagg aggccatgct caggtccagg cccatgatct
    26761 gcaatgccgc ccggacgtcc tgctgggcgg ccactttccg ctccactccc gcggaagact
    26821 gttgccccgc agtcatggcg acataggcgg cccccaacaa cagcatcccc accaaaatgg
    26881 cgaacatgac ttcgatcagg gtaaagccta tttcttttcc cctcatat
//
LOCUS       DASPRR010000240         3722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_862062, whole genome shotgun sequence.
ACCESSION   DASPRR010000240 DASPRR010000000
VERSION     DASPRR010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862062"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VEL68_09335"
     CDS             <1..313
                     /locus_tag="VEL68_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXZ36209.1"
                     /translation="DQLPFGKEALDFVVASHNLEHYEDIQKTLGEWKRVVKNGGIIGM
                     VVPDDRIIDTRALNPQHRHALTPELLEDQIKKSGGLEIEVLQEIVDAWSFGCICRKIS
                     Q"
     gene            367..2250
                     /locus_tag="VEL68_09340"
     CDS             367..2250
                     /locus_tag="VEL68_09340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ36210.1"
                     /translation="MIEPICDIIIPIWNQLSLTQRCLEAVRKKTRTPHRLILIDNGSE
                     EGTRKYLEGLAESPSWTGVLVRNEKNRGYIQAVNQGLKISTAPYVCLLNNDIVVTAGW
                     LERMIEFAGSHPEVGLVNPQQNHDPGKPMPDDLEGFAASQVDTRWGWMELDHCTGGCL
                     LIKREVIEKIGFLDETYGQGYYEDNDYSRRAQRAGYRCLRLLDTFVWHDIEGSFKEDP
                     DRGEKRRKNEALYQSRWGNPLRIIYPIHEGVDFRRARFHQVFQTVHALARQNCEIDLI
                     LGRNRVNVPSEGLAHYGLWPHENLRIHLLPTLRREAGRFLRVSWDGVFLWGCLLKVRE
                     LLRQRGYNAIYTRHLNTASFFLRFKKYLRLPIVFEAHEIFFLTTDRHDKREKIKREEF
                     RTYPRLDGIVFITQGLEQRMKEVFSLQAPMAVVPDGVNLDFFGGVARQPRSQKIIYVG
                     QLYPWKGAGTLVEAMQYLATGELHLVGGSEERVQELKEKAEQLGVGKRVWFHGQVSPQ
                     EVKNHLAEAAVAVLPLPKDLISASFTSPLKLFEYMAARLPIVASDLPSTREVLSPGVN
                     ALLVPPNDPQALGEGIQRLLEDRSRAEGLGQKAYEGVMEFTWEKRAQKISHFIRSLSR
                     EGS"
     gene            2247..3320
                     /locus_tag="VEL68_09345"
     CDS             2247..3320
                     /locus_tag="VEL68_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXZ36211.1"
                     /translation="MSFWVEKLFHFLIHRRWNLDGGTFDPGRVQRILVVRSDNVGDVL
                     CCTPAIRALRRAFPRAYLAALVVAYSRDAITGNPNLDEVFVYEKAKHRPDQSRMVSLL
                     KQFQVESRLKKKGFDLVIGLRSSFSWAEGWLVYFTGAPFRLGYDPGEGRNQKFGFFYN
                     LRVPAAPQAHEVERALYLVGTLGVQPAEKNLTVVIPEEDRGAAESFLQQKKIDPRNLI
                     GFHLSSRLPANRWSPEKFAGLATRLIREDGKTVVLTWGPGDEARAEEVQIKSGDGTFL
                     FPTPTFKRLGAIQQQCRAFVSPDGGAMHFSTAVGTRTVGLFGKSKPEDWAPWGDGHVA
                     LRRGPQAELISVEEVYQALERWL"
BASE COUNT          809 a         1012 c         1104 g          797 t
ORIGIN      
        1 ggaccagctt ccctttggaa aagaagcgct ggatttcgtc gtggcgtccc acaacctgga
       61 gcactacgag gatatccaga agaccctggg ggagtggaag cgggttgtaa aaaatggcgg
      121 gatcatcggt atggtggtcc ccgatgatcg gatcatcgat acccgggctc tgaaccccca
      181 gcacagacac gccctgaccc ctgagctctt ggaggatcaa attaaaaaaa gcggggggct
      241 ggagatcgag gttctccagg aaatcgtcga cgcctggagt ttcggctgca tttgccgaaa
      301 aatttctcag taggggaacg ctatagcatg cccctaacat ccgcataacc ctgaattatt
      361 ggtcttatga tcgaaccgat ctgcgacatc atcattccca tctggaacca gctgtctttg
      421 actcaacggt gcctggaggc ggtccggaag aagacccgaa cgccccaccg cctgatcctg
      481 atcgataacg gaagcgaaga agggacacgg aagtatctcg agggtctggc cgaaagtccc
      541 tcctggaccg gggtcttggt ccgaaacgaa aagaaccgcg gttacatcca ggcggtcaat
      601 cagggcctga agatttccac cgccccctac gtctgccttc tgaacaatga catcgtggtg
      661 accgctgggt ggctggaacg gatgatcgaa tttgccgggt cccatcccga ggtcggcctg
      721 gtgaaccccc agcagaacca cgaccctgga aagcccatgc ccgacgatct ggaagggttt
      781 gccgcatctc aggtggacac ccggtggggt tggatggagc tggaccattg cacgggtggc
      841 tgtcttctga ttaaacggga agtcatcgaa aaaatcggat tcctggatga gacctacggg
      901 cagggatatt acgaggacaa tgattattcc cgcagggccc agagggctgg ctaccgctgc
      961 ctgcgcctgc tggacacctt tgtctggcat gatatcgagg gttccttcaa agaggacccc
     1021 gatcgggggg agaagcggag gaagaatgag gccctgtacc agtcccgttg ggggaatccc
     1081 ctgcggatta tctacccgat ccacgaaggg gtcgattttc ggcgggcccg tttccaccag
     1141 gtcttccaga cggtccatgc cctggcgcga cagaattgcg agatagacct aatccttggg
     1201 aggaatcggg tgaacgtccc ttccgaaggg ttggcccatt atgggctttg gccccacgaa
     1261 aacctgcgca tccaccttct tcccacgttg cgccgagaag caggccggtt tcttcgagtt
     1321 tcctgggacg gagtttttct ctgggggtgt ctgctgaagg tgcgcgagct tctgcggcaa
     1381 agggggtata atgccatcta tacccgccac ctgaacacgg cctcgttctt cctgaggttc
     1441 aagaaatatc tccgccttcc cattgttttt gaggctcatg aaatcttctt ccttaccacg
     1501 gaccggcatg acaaaaggga aaagatcaaa agggaggagt ttagaaccta cccccgcctc
     1561 gacggaatcg ttttcatcac ccagggacta gagcagagaa tgaaggaagt cttttccctc
     1621 caggctccca tggctgtggt gcccgatggg gtcaatttgg atttctttgg gggcgtcgcc
     1681 cggcaaccgc gaagtcaaaa gatcatttac gtggggcaac tctatccatg gaagggagct
     1741 gggacccttg tggaggctat gcaatatttg gccaccggag aactccatct ggtagggggc
     1801 agcgaagaac gggtccaaga gctcaaggag aaagcagagc agctgggtgt ggggaaacgg
     1861 gtttggtttc acggtcaggt ttccccgcag gaggtgaaaa atcatctggc agaggcggcc
     1921 gtggcggtcc tgcccctgcc gaaggacctc atttccgcct ccttcacctc ccccttgaaa
     1981 ctcttcgagt acatggccgc ccgccttcct atcgtggcct ccgaccttcc ttcgacccgg
     2041 gaggtcttat cccctggggt gaatgcgctt ttggtcccgc ccaacgatcc gcaggccttg
     2101 ggggaaggga tccagagact gcttgaggat cgctctaggg ctgaaggcct tggccaaaaa
     2161 gcctatgagg gcgttatgga atttacctgg gagaaacggg cgcagaagat ctcccatttt
     2221 atccggtccc tatctaggga gggatcgtga gtttctgggt cgaaaagctt tttcatttcc
     2281 tgatccaccg gagatggaat ctggatgggg ggactttcga ccctgggcgg gtccagcgca
     2341 tcctggtggt tcgcagcgac aatgtgggcg acgtcctttg ttgcaccccg gccatccgtg
     2401 ccctgcgaag ggcttttccg cgggcctacc tggccgcttt ggtggtcgcc tacagccggg
     2461 acgccataac cggaaaccct aacctagatg aggtctttgt ctatgaaaag gccaaacacc
     2521 ggccggacca aagccgaatg gtctccctcc tcaaacaatt ccaggtggag agtcgcctga
     2581 agaagaaggg cttcgatctg gtcatcggcc tccggtcctc cttttcctgg gccgaaggtt
     2641 ggctggtcta cttcaccgga gctccctttc gcctaggata cgacccgggg gaggggagaa
     2701 accagaaatt tggattcttc tacaaccttc gagttccggc tgcgccccag gcgcatgaag
     2761 tggagagggc tctttatctg gtgggaaccc tcggggttca gcccgcggag aagaatctga
     2821 ccgtggtgat tccagaggaa gacagagggg cggccgaatc cttcctacag caaaagaaga
     2881 ttgatccccg gaacctcatc ggcttccatc ttagcagtcg ccttccagcc aaccgatgga
     2941 gtccggaaaa attcgccggc cttgccactc gcctcatccg ggaggatgga aagacggtgg
     3001 tgctgacctg gggtccggga gacgaagcca gggccgaaga ggtacaaata aagagcgggg
     3061 atgggacctt tcttttcccc accccgactt tcaagcgtct aggggccatt cagcaacaat
     3121 gccgggcttt cgtaagcccc gacggcggcg ccatgcactt ctctacggcc gtgggaacac
     3181 gcaccgtggg gctattcggg aagagcaagc ccgaagactg ggctccctgg ggggacggac
     3241 acgtggccct gcgaagaggc ccgcaggccg aactgatttc ggtggaggaa gtgtaccaag
     3301 ctttggagag atggctgtag tcgatttgaa atgaattttc cttgcgggaa acccccgact
     3361 ttgctgcgca tatccggaat ctggcattcc tgggatgttt ggggactggt gccgggaaga
     3421 gggcattgcc cgcagaaaac gtcggccgaa agggcccttc tctaatctgc tttgagcggt
     3481 tacttttcat caaacctccg ggtccgacgg agctggtgac cttcggatta ccgattaccg
     3541 aaagcaaatt ttgagttttg agcagaagga tgaaatttcc gatcgaccgt tgggtaaaga
     3601 accttctctt tcatacctat gcccctttgg gttgggctcg ttctcccaac cacgtgagcc
     3661 tggatgtcac ccgccgctgt aacctaagat gccggatgtg tttctattac ggaggagagg
     3721 ac
//
LOCUS       DASPRR010000241        11887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_702749, whole genome shotgun sequence.
ACCESSION   DASPRR010000241 DASPRR010000000
VERSION     DASPRR010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11887)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702749"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..234)
                     /locus_tag="VEL68_09350"
     tRNA            complement(142..234)
                     /locus_tag="VEL68_09350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ser,seq:gct)
     gene            complement(392..479)
                     /locus_tag="VEL68_09355"
     tRNA            complement(392..479)
                     /locus_tag="VEL68_09355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Ser,seq:tga)
     gene            complement(589..1881)
                     /gene="serS"
                     /locus_tag="VEL68_09360"
     CDS             complement(589..1881)
                     /gene="serS"
                     /locus_tag="VEL68_09360"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXZ36212.1"
                     /translation="MLDLKYTREHLEEVKEKIGRRGQTVDWERFAQLDAERREILQES
                     ESLRAQRNQVSEVIAEKKKKKQDAQEEIGRMKEVSNRIKELETRLGEKEEALQGLMLT
                     IPNVPHGSVAVGQGEQDNPEIRKWGKLPEFSFNPQPHWDIGEALDILDFNRGAKITGA
                     RFTLYKGLGARLERALLNFMLDLHTSEHGYQEVLPPFLVNRKSMTGTGQLPKFEEDLF
                     KLSDPDYFLIPTAEVPVTNIHQDEILAEEELPLYYAAYTPCFRKEAGSYGKDTRGLIR
                     QHQFNKVELVKFTTPESSYEELEKLTRNAEEVLRRLGIPYRVVMLCTGDLGFSASKTY
                     DLEAWLPGQGVYREISSCSNFEDFQARRANIRYRPKGKKGTEFVHTLNGSGLAIGRTL
                     VAILENFQQEDGSVLIPEALRPYMGGTTKIERHIAHRA"
     gene            complement(2034..2669)
                     /locus_tag="VEL68_09365"
     CDS             complement(2034..2669)
                     /locus_tag="VEL68_09365"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXZ36213.1"
                     /translation="MSNETLFLLHYSEPFIYAGLFLILFLCGLGLPIPEELTLLTGGF
                     FVNLGITRLYPTLAVGFLGILGGDLAIYAIGKKWGQDLLHHPHLRKVITDSRLEKGRQ
                     FFRDHGEKTVFIARFISGFRVAAFFAAGTMGIKLGRFLLLDFLGALILIPLLLLLGYY
                     FGASIGWLAEIITQIDYLLKILAVLACIAALVFYLWLRKKAPAGRKIPPKT"
     gene            complement(2700..3029)
                     /locus_tag="VEL68_09370"
     CDS             complement(2700..3029)
                     /locus_tag="VEL68_09370"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXZ36214.1"
                     /translation="MIRIILAVLFVILVATFSASNREEISLRYFFGWSTGSFPIFLLI
                     LISLVAGMVLGFSVDWGERRKLRNKARRLGVQVKQLRKEIETRTAEETPPEPPSSLSE
                     APKTTPG"
     gene            complement(3410..3787)
                     /locus_tag="VEL68_09375"
     CDS             complement(3410..3787)
                     /locus_tag="VEL68_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXZ36215.1"
                     /translation="MFAKDREKMESLLGSSSNFKGDLNVKGTLRVDGRAEGRLDADCV
                     ILSESAMVKGEIQGKKIIIGGTVEGNLRAQELVEIKSKGKVLGDIFSQKLVVIEGAEF
                     NGRIETKKMEGNIIGLEFRSQEA"
     gene            complement(3787..4623)
                     /locus_tag="VEL68_09380"
     CDS             complement(3787..4623)
                     /locus_tag="VEL68_09380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXZ36216.1"
                     /translation="MERIHFLFKKALTPVTIMVIPHETLKSLNLKVPLLGILLSLLLL
                     GVGAVQTYRVAVNGLQYAIMVEKVNYYAQQFSQWNSTVTALKETEEDFRRIFSLTSKD
                     QVLETIDTSYSGDIDIQNLMAELQKSVEKIDEIKDFLRIQKDLYLATPKGYPTEGNIS
                     SRFGNRESPFTGIRTFHSGVDICASPGSPVWATADGVVSHSGWTQYSGYLVAIEHGCG
                     FSTIYAHNKKNAVKIGQRVKRGDIIGYVGSSGRSTGPHVHYEVWEKGKSVNPHKFLQG
                     RS"
     gene            5006..5197
                     /locus_tag="VEL68_09385"
     CDS             5006..5197
                     /locus_tag="VEL68_09385"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HXZ36217.1"
                     /translation="MQPPDDRHLELLGAITGAKTEGRLGGPVLAIWTFRAKEPGTMEA
                     KMHYYRSWEGAGRAGETKI"
     gene            complement(5200..5448)
                     /locus_tag="VEL68_09390"
     CDS             complement(5200..5448)
                     /locus_tag="VEL68_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36218.1"
                     /translation="MKITINSVEQNFPVGTKLIELVIRVREAHRDNPVLKSLVEKTGK
                     DHITFSHNRRVVKPSEYGSIELKEGDEVRWMLPYAGGS"
     gene            complement(5672..7549)
                     /locus_tag="VEL68_09395"
     CDS             complement(5672..7549)
                     /locus_tag="VEL68_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ36219.1"
                     /translation="MKGVWGKVLKVDLTNGTCKAETLPEQTYQYFLGGAGLAAYYMWK
                     ECPAGTTAFNPANRLTFAGGPMTGLKQTGAAKWTAGAISPAIHMNADSAATASFGIEM
                     KETGFDAIVVYGRSPKPAYVVVQDGKARIKDARKFWGKDAYETEDGIKEAEGDGFEVV
                     TIGQAGERLVRYANIQTHKKSFLGRCGLGAVMGSKMLKGIAVQGSQNCPVHDAAEINR
                     LNKDLNRRVAELDAAQPEFLQFKRLGTAGATELFAPQGNLPIKNYSLARFPSGEKNLH
                     GREYVRLLNAKPWPCKYCVMQCHNLCEIKKGPYAYKGKGPEYESFAMMGFNTMVDDME
                     AVAYACELANLYSMDCISLGVVIAWAMESYEKGVISQKDTYGLDLKWGSAEALVEITK
                     KIGERADGLGYLLGEGCKIASDVLGKNSGDWAVQMKGQEIAAHNWRAQYISALNYCTG
                     VASGPNHERGNSQHIWVAHVRLPEWGIDEVKTEERWSWEKAPERNAIFHDYCNVINSA
                     CHCKFHEFRGYTLTDMLNSINASTGWGWSIQDLRTCGERITTLQKFLNIRYGWQKEHD
                     FQYPKRFMEPVHEGPAAGKIPVGLDQAILEYYRVREWDESGRPTQQLIKRLGMGEFAG
                     E"
     gene            complement(7781..8614)
                     /locus_tag="VEL68_09400"
     CDS             complement(7781..8614)
                     /locus_tag="VEL68_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36220.1"
                     /translation="MLLTIFYGFSIGAILYFISIGLSLTFGTMRVINFAHTLTYSIGV
                     YVLITCIPWIKGNFIPAAILAILAVIPISYVIERFVIRRLYGESLDYTFIATFAITLM
                     GVDTIKWVWGVNPLPLSPPLGTYIEFWGLTFPVYRIIIFAIAIVLFLGLELFFKRTIM
                     GKIVVAALEDKDGVRCLGVRVDKYFSIMFILGSALAGLGGVLYAPISAVHPYMGLSVL
                     LLAFAVVIVGGMGNLRGTFISAFSLGMVMAITARYWSQGAETMVFIVMAVVLFFRPID
                     I"
     gene            complement(8617..9636)
                     /locus_tag="VEL68_09405"
     CDS             complement(8617..9636)
                     /locus_tag="VEL68_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36221.1"
                     /translation="MNPTSMRIAEPSPDQDFSPKRERKGLEILIIGIGFLILIPLISL
                     HRVTDFMIFCIFALSFDLLYGYMGRLSFGHLLYLGTGAYVFGLFIKYFQCNAFLAIFC
                     GVLAAGLLGMLLGLIMVRTTGACFSLMALAFNQIGFFLVLSPLKGITDGENGFGAHAR
                     NIGVLNFNSKITLFILTLLLLLLVFYFLKRLTTSPYGILVRSIKEDENRVRFLGYNTY
                     FYKWVAFVTASLIAGLAGTLTALNYRFVNPNVMDVHQNVGVVFACLIGGAGNLYGAII
                     GGVAYMMISNFLAIYIQRWEMFLGIALLIIVFRFRKGIWGSIQNIGEYFQRPKEVQPV
                     QEKRA"
     gene            complement(9666..10355)
                     /locus_tag="VEL68_09410"
     CDS             complement(9666..10355)
                     /locus_tag="VEL68_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36222.1"
                     /translation="MLEIKNLEVAYGHAKVLHDVSFRIEKGEMGFFIGRNGAGKTTLL
                     KTIMGLLKPQKGSIHYRDQEITGKSPEELYPLGLRYVGQEKKVFGNLTVRENIEVAAF
                     GSQESMDHALEKVMALYPKLKNFLHIKAGQLSGGQRQILLVGRALVGDPKFLLIDEPT
                     QGLAAVVIEDIGVILKKLKEQVSAVIVEQNLAIVKYLADRVYILKEGKLAKEIGEKSQ
                     ITDTQGMEEYL"
     gene            complement(10458..11225)
                     /locus_tag="VEL68_09415"
     CDS             complement(10458..11225)
                     /locus_tag="VEL68_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ36223.1"
                     /translation="MAEVLKAEGLSKHFGGLVAVNQVSFQIQPNESVGIIGPNGSGKT
                     TFFNLLTGLFPPTQGQIHFFRKDITDLAPNQRVLQGMVRTFQLVSVFNSLTVWENLVL
                     SSLRFRRESLSEWGFLFSPANREDIRESCFEAMKGVGLEKNWQTVTSELSYGSKRMLE
                     IGIALSLRPKLLLLDEPLSGLSDLEIVEVLDILHRIKKDLTLIIIEHKLSKIVNLVDR
                     LCVMNEGSFICEGVPDQVLSDRSVRECYWGKGEVTLS"
     gene            complement(11318..>11887)
                     /locus_tag="VEL68_09420"
     CDS             complement(11318..>11887)
                     /locus_tag="VEL68_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36224.1"
                     /translation="AKPDVVMMGHWGNDAINALKQVYELGLRKESKIVFNSIGYTLAS
                     GVPADALDGVTMGWWWYHDLSGLKDPATEKATNEFVQKYLKEFGDVPDSFATFTYLAI
                     METLRGVQTSNSTDPAKVYKAIMDKPEFMGPKGPAKWRIDGRPDYKYVYYIMDGKGAK
                     ARKDRSDIAKMVEVYTGPELCLPLKEMGY"
BASE COUNT         2760 a         3285 c         2988 g         2854 t
ORIGIN      
        1 ggatttttgc gttaggtctt tatttccaac cactaacgtg agtggggaaa tcccagaacc
       61 tgagaggtaa tgtcaaccgc agccaccaga ggatgtgccc cgaatgcgcc ccagtttttg
      121 cggctgagtc tttgaaatta ctggcggaga gagcgggatt cgaacccgcg gtacaccttt
      181 cggcgtacac tcgcttagca ggcgagcacc ttaggccgac tcggtcatct ctccgcgatc
      241 gtgccggtgg cacgataccc ttgacaattt aacctttaac aatgaacttc cggtagttcc
      301 tcgacgcagt aattattaat ttggactcat tcttctaaag ctataagtca aaagttaata
      361 gttaagtata aaatttgcac agcaaatttt ctggcggagg gagtaggatt cgaacccacg
      421 tcccctttcg gggaaacggt tttcaagacc gccgccatcg accactcggc catccctccg
      481 gagacattca aaatcgcata gggcaaagag catagagtga gggcaaaaaa caaaaaatta
      541 ccacgaaaat ctttgagcaa tgagctatga accgtcttac gctatgccct atgctctatg
      601 ggctatgtgt ctttcgattt tggtcgttcc gcccatgtaa ggcctcaaag cctcgggaat
      661 aagaacgctt ccgtcttcct gctggaagtt ttccaggatg gccaccaggg ttcgacctat
      721 cgccagcccc gagccattca gggtatggac aaactcagtc ccctttttgc ctttgggacg
      781 gtaacggatg ttggccctcc gcgcctggaa atcctcgaag ttgctgcagg aggagatctc
      841 cctgtaaacc ccctggcccg gtagccaagc ttccagatcg taggttttgg aagccgagaa
      901 acccaagtcc cccgtgcaaa gcatgacgac ccggtaggga atccccaggc gcctcaacac
      961 ctcttctgca ttccgggtca gtttttcaag ctcttcgtat gagctttccg gggtggtgaa
     1021 tttcactagt tccactttgt tgaactgatg ctgccggatc aacccccggg tgtctttgcc
     1081 gtaggagccg gcttctttgc ggaagcaggg agtataagcc gcatagtata gaggaagctc
     1141 ctcttccgcc aatatttcat cctggtgaat attcgtcacc ggtacttccg cggtggggat
     1201 caagaaataa tccggatcgc tgagtttgaa aaggtcctct tcaaatttcg gtaattgccc
     1261 ggttccggtc atgctcttgc ggtttaccag gaagggagga agaacctcct ggtagccatg
     1321 ttcggaagtg tgcaggtcca gcatgaaatt cagtaacgct ctttccagcc tggcccctaa
     1381 tcccttgtat agagtaaacc gggctccggt tatcttcgcc ccccggttga aatccaaaat
     1441 atcgagcgcc tcaccaatat cccaatgagg ttggggatta aaagagaatt cagggagctt
     1501 tccccatttg cggatctcag gattgtcctg ttccccttgt ccgacagcaa cgctcccgtg
     1561 ggggacgttg gggatggtca gcatgagccc ctgcagggct tcctctttct cccccagccg
     1621 agtctccagc tccttgatcc ggttggagac ttccttcatc cggccaatct cttcctgggc
     1681 atcctgcttt ttctttttct tctccgcgat cacctcggag acttggtttc tctgggcccg
     1741 aagggattcc gattcctgga gtatttccct tcgttcggca tccagctggg caaaccgctc
     1801 ccagtcgacg gtttggcccc gccgcccgat tttttctttt acttcctcga ggtgttcacg
     1861 cgtatatttc aggtccagca taaggtaatt cctcgatttc cccgtaacct taagtaaatt
     1921 atcatttcgt ggaggcagag tcaactctga tgttccgctc ttcatggccg atcagggaaa
     1981 gagggaaaaa gcttgagcgg aaagcgcaaa ggggggggac agcaaacgga tggctaggtt
     2041 ttgggaggaa ttttccttcc cgcgggagcc ttttttctca gccaaagata aaagaccagt
     2101 gccgcgatgc aggctaaaac agccaggatc ttgagcagat agtcgatctg agtaatgatc
     2161 tccgccaacc atccgatgct cgccccaaaa taatatccca ggaggagcag caggggaatt
     2221 agaatgagcg ctcccaggaa gtccaggagc aaaaacctgc ccagtttgat ccccatcgtc
     2281 ccggcggcaa aaaaggcagc gacccggaac ccggaaataa acctggcaat aaagaccgtc
     2341 ttttccccgt gatcgcggaa gaactgccgg cccttctcca ggcggctatc ggttatgact
     2401 ttgcgcaaat gggggtgatg gagaaggtcc tggccccatt tcttcccgat ggcgtaaatg
     2461 gccaggtctc cccctaggat ccccagaaaa ccgacagcca gagtggggta aagccgggtg
     2521 atgcccaggt ttacgaagaa gcccccggtc agcagagtca actcttctgg aatgggaagc
     2581 cccaagccgc agaggaatag gatcaagaag agcccggcgt agatgaatgg ctcagagtaa
     2641 tggaggagga aaagggtttc gttagacaag gtttcttggg ctccagccgc tgaatcacct
     2701 tatcctgggg ttgtttttgg ggcttctgag aggctggaag gtggctcggg gggagtctcc
     2761 tccgcggtac gggtttcaat ttctttgcgg agctgcttca cctggactcc caatctccgc
     2821 gccttgttcc tcagtttccg cctctccccc cagtctaccg agaatcccaa caccattccg
     2881 gcaaccagcg aaatcaggat gagaagaaaa atgggaaagg accccgtact ccagccgaag
     2941 aaatagcgca gggagatttc ctcccgattt gaggcagaaa aagtagccac caggattaca
     3001 aaaaggaccg ccaagatgat tctgatcaat ccatcacccc aaaggttaaa ggtctcttta
     3061 ttcaattttt agcgctttta aaagatagca aatcctttaa gagagtgtca actcatttta
     3121 atccaagtcg aggttgattt ccttggtaga ctgcaggctc ggttccattc tccccgaagg
     3181 atttgaattc cctcggggtc ggtgcgctcc agatcaagga tggacggatt tgccgggctt
     3241 gagaagggaa agatgggacc acgggcacca gggcgggcat cactcggagt gaaggccttg
     3301 gccccttcga gaaaggtggg acaattattt cagcttgtag gaagcaaaga attcggaaaa
     3361 aggggaggag gggtcaatct tcggtagggt cgggggacgg aacctagact caggcctctt
     3421 gacttctgaa ttccaatccg atgatattcc cttccatctt ttttgtttcg atccttccgt
     3481 tgaactccgc cccttcgatg accactaatt tctggctaaa aatatctccc aggaccttgc
     3541 ctttggattt gatttccacc agctcctgag ccctaaggtt tccttccacc gttcccccga
     3601 tgatgatttt tttgccttga atctcccctt tcaccattgc gctttcactc aggatcacgc
     3661 agtcggcatc caatctcccc tcggctcggc cgtctactct caaggttcct ttcacattca
     3721 agtccccttt aaaattggaa ctcgacccca gcaaggattc catcttctcc cgatctttgg
     3781 caaacatcac gatctcccct gaagaaattt atgggggttt acactcttcc ccttctccca
     3841 tacttcgtaa tgaacgtggg ggccggtcga tcttccgctg gaccccacat aaccgatgat
     3901 gtctccccgt ttcactctct gccctatctt gaccgcgttt ttcttgttgt gcgcatagat
     3961 ggttgaaaac ccgcacccgt gttcaatggc taccagatat ccactgtact gagtccaacc
     4021 cgaatgactc accactccgt ccgccgttgc ccaaacgggg cttccggggc tggcgcaaat
     4081 atccaccccg gaatggaaag tccggattcc ggtaaagggg ctctctcggt ttccaaaacg
     4141 cgagctgata tttccttctg tcgggtaccc ttttggggtg gccaggtaga ggtccttctg
     4201 gatgcgcaga aagtccttga tttcgtcgat cttttcaacg cttttttgga gctccgccat
     4261 gaggttctgg atatcgatgt ccccggagta ggaagtatcg atcgtctcca ggacttggtc
     4321 tttggaggtg agggaaaaaa ttctgcggaa atcctcctcc gtttctttca aagcggtcac
     4381 ggtggaattc cactgggaga attgttgagc gtaataattc accttctcca ccattatggc
     4441 atattgcagc ccgttaaccg ccacccggta ggtttgaacc gcccctaccc ccagaagtag
     4501 taaggatagt aggattccca atagggggac cttgagattc agggatttca gggtctcatg
     4561 gggaatgacc atgatggtga ccggagtgag cgcctttttg aacagaaaat ggattctttc
     4621 catagtcatg actcttgcgg ttagcgattt tttttcttct tcttcttcac ttcgggtagg
     4681 ccttccctag gctatttttg cccctcttcg ggcaacgcga tgtacatttt tgacctacag
     4741 gagccttttt gagttcaaga ttcgtccatg aaaccattca aaccaggacc taaaactgct
     4801 taaactctaa gaccttattc attttcttaa atcaccatgg gcaaaatgcc aacttttcta
     4861 ttgtagaata gctctgcaag cactgtgcca tttcctgggc aaaccgaaga taagatgacc
     4921 gcagtctgtc ttgcctaatt gccgaggcaa gaggcaatag gataccccta ccttccggtg
     4981 aaagttcagg aaaaggcccg caaggatgca gccccccgat gaccggcatc tggaacttct
     5041 aggcgcgatt accggggcca agactgaagg ccgcctggga gggcctgttc tagccatctg
     5101 gacttttcgg gccaaagagc ccgggacgat ggaggcaaag atgcattact accggagctg
     5161 ggaaggggcg gggagagcgg gggaaacaaa aatttaaccc tatgaaccgc cggcgtaggg
     5221 aagcatccag cggacctcgt cgccttcctt gagttcgatg gaaccatatt ccgagggttt
     5281 gacgacacgg cggttgtggc taaaggtaat gtgatccttg ccggtcttct cgaccagtga
     5341 tttcagaacc gggttgtccc tatgggcttc ccgcacccgg atgaccagct cgatcaattt
     5401 tgtccctacg gggaaatttt gttctacgga gttgattgtt attttcatgg gccggaaaga
     5461 ttcaccccca ccctgtcctt cactcgaggc tatggcataa tgggaaaagg catcattccg
     5521 cgaaaagcgg gaatccaggt tcttgaatag ttcgttgtct tcaaatgact ggatgcccgc
     5581 ccttcaccat actaggtaca gggtccgcct ccgcgagcat cacaaattat gaccctatgt
     5641 ctttatgggg gacggattct tttgcagttt attactctcc tgcgaattcc cccatgccca
     5701 gccgcttgat cagctgctgg gtcggccttc cgctctcatc ccattccctc acccggtaat
     5761 actccaggat agcctgatcc aagcccaccg ggatcttgcc cgccgccggc ccttcgtgaa
     5821 cgggctccat gaaccgtttg ggatactgaa agtcgtgctc tttttgccag ccataccgga
     5881 tgttcaggaa tttctggagg gtggtgatcc gttccccgca tgtgcgcagg tcctggatgc
     5941 tccagcccca cccggtggag gcgttgatgc tgttgagcat gtcggtcagg gtgtaccccc
     6001 ggaattcatg gaatttacag tggcaggccg agttgatcac gttgcagtag tcatggaata
     6061 tggcgttccg ttccggcgct ttctcccagg accagcgctc ttccgtcttc acttcgtcga
     6121 ttccccattc gggcaaccga acgtgagcca cccagatatg ctgggagttg ccccgttcgt
     6181 ggttggggcc ggaagccacg ccggtgcagt aattcagggc cgagatgtac tgggcccgcc
     6241 agttgtgggc ggcaatctcc tgccctttca tctggaccgc ccaatcgccc gagttcttcc
     6301 ccaaaacgtc ggatgcgatc ttgcacccct cgcccagaag gtatcccaaa ccgtcagccc
     6361 gttctccgat cttcttggtg atctccacca aggcttctgc acttccccat ttcaggtcaa
     6421 ggccataggt atccttctgg ctgatgaccc ccttctcgta ggattccatg gcccaggcga
     6481 tcaccacccc aagggaaatg cagtccatgg agtaaaggtt ggccagctcg cacgcgtagg
     6541 ccacagcctc catgtcgtcc accatggtgt tgaagcccat catggcgaag gattcatact
     6601 ctggaccttt acccttgtag gcataggggc ctttcttgat ctcgcagagg ttgtgacact
     6661 gcatgacgca gtacttgcag ggccagggtt tggcgttgag caatctcaca tattccctgc
     6721 catgaaggtt cttctcgccg ctggggaagc gagccagaga ataattcttg atgggaaggt
     6781 ttccctgggg ggcgaagagc tccgtggccc cggcggttcc caaacgctta aactgcagga
     6841 actccggctg ggcggcgtcg agttctgcta cgcgccggtt taggtctttg ttaaggcggt
     6901 tgatctccgc agcgtcgtgc accgggcagt tctgagaccc ctggaccgca atgcccttga
     6961 gcattttgga gcccatcacc gctcccaggc cgcagcgccc caggaaagat ttcttatggg
     7021 tctggatgtt ggcatagcgg accaatcgtt ccccggcctg gccgatggtg accacctcaa
     7081 aaccatctcc ttccgcctcc ttgattccgt cctcggtctc gtaggcgtct ttcccccaga
     7141 acttccgggc atctttaatc ctggccttgc cgtcctgcac aactacgtaa gcaggtttgg
     7201 gagaccggcc gtacaccacg atggcgtcaa aaccggtctc cttcatctcg atcccgaaac
     7261 tggccgtggc cgccgaatcg gcgttcatgt ggatggcagg agagatggct ccggccgtcc
     7321 acttcgccgc cccggtctgc ttcagcccgg tcatgggacc cccggcaaag gtgagccggt
     7381 tggccgggtt gaaggccgtc gtgcccgccg ggcattcctt ccacatgtag taagcagcca
     7441 gccccgcacc gcccagaaaa tattgatagg tctgttccgg cagagtctcc gccttgcagg
     7501 tgccgttggt caggtccacc ttcaatactt ttccccatac gcccttcata agtacctcct
     7561 ttctctcttt tactcgggta accaagaatg cgggaaagga ttccccccct tgaaaaccct
     7621 acggagaaat tccaaattcc aatctccaaa taccaaataa attccaatgc gcaaattcca
     7681 gagctccaat accttcaaac cctctaagtt cgatccttga atttttggat ttcattggaa
     7741 tttggtgctc gtttgttatt tggtgcttga gatttggact ttatatatca atgggtctga
     7801 aaaacagcac gacggccatc acaatgaaca ccatggtctc cgccccctgc gaccagtagc
     7861 gggcagtgat ggccataacc atgcccaggg agaaggcgga gataaaggtc ccccgcaggt
     7921 ttcccatgcc acctacgatc accacggcaa acgccaacag gaggaccgaa agacccatgt
     7981 aagggtgaac ggcgctgatg ggggcataaa ggaccccccc caatccggcc agggcgcttc
     8041 ccaggatgaa catgatggaa aaatatttat ccacccggac tcccaggcat cgcaccccgt
     8101 ctttgtcctc tagggcggcc acgacgattt tgcccatgat cgttctcttg aaaaagagct
     8161 ccagccccag aaagaggacg atggcgattg caaagatgat gatccgataa acgggaaagg
     8221 tcagtcccca gaattcgata taggtcccga gaggaggact caagggaagg ggattcaccc
     8281 cccagaccca tttgatggta tccacgccca ttagggtgat ggcaaaggtg gcgatgaaag
     8341 tgtaatccag ggactcgcca taaagcctcc ggatgacaaa gcgctcgatg acatagctga
     8401 tcggaatgac ggccaaaatc gcaaggatcg cggcggggat gaaatttcct ttgatccagg
     8461 ggatgcaggt gattaaaaca taaacgccga tgctgtaggt taacgtatgg gcaaagttga
     8521 tgactcgcat ggttccaaag gtcagagaga gcccgatgga aataaaatag aggatcgcgc
     8581 caatggaaaa tccatagaaa atcgtcaaaa gcacgcctaa gccctctttt cttgtaccgg
     8641 ttggacttct ttgggccttt gaaaatattc accgatgttc tgaatacttc cccagatccc
     8701 cttgcggaat ctgaacacga taatgagcag ggcgatcccc aggaacattt cccaccgctg
     8761 gatatagatg gccaggaagt tactgatcat catgtacgcg actcccccga tgatcgcccc
     8821 gtagagattc cccgcgcccc cgatcaagca ggcgaagacc actcccacat tctgatgaac
     8881 gtccatcaca ttcgggttga caaatcggta attcaaggca gtcagggttc ccgcaagtcc
     8941 ggcgatcagc gaagcagtaa cgaacgccac ccacttgtaa aaataggtgt tataccccaa
     9001 aaaccggact cgattttcgt cttctttaat ggagcggacg aggattccat agggagaagt
     9061 ggtcagccgc tttagaaaat aaaagaccag aagaagcaac agcaaagtca gaataaacag
     9121 ggtgatcttg ctgttgaaat tcaaaacacc gatgttccgg gcatgggcgc caaaaccgtt
     9181 ctccccgtcg gtgattccct tcagaggaga gaggaccaga aagaagccga tctggttgaa
     9241 tgccagggcc atgagggaga aacaggctcc cgtcgtccgg accataatga ggccaagcaa
     9301 cattcctaag aggcccgccg ccagcactcc gcaaaaaatc gcgaggaagg cattacactg
     9361 gaagtatttg atgaaaaggc caaaaacgta ggcgccagtt cctaaataga ggagatggcc
     9421 gaaagaaaga cggcccatat atccataaag aaggtcaaag gatagggcga aaatgcagaa
     9481 aatcatgaag tccgtgacgc ggtggaggga gatcagagga atcaaaatga gaaagccaat
     9541 cccgatgatc aaaatttcca gtcccttccg ctctcttttt ggcgaaaaat cctgatccgg
     9601 gcttggttcg gcaatccgca tggaagtcgg gttcaaagta gactccttct gacgatggag
     9661 aagaatcaga ggtattcctc cattccctgg gtatccgtga tctgcgactt ttctccgatt
     9721 tccttggcga gctttccttc tttgaggata tacactcggt ccgccagata tttcacgatc
     9781 gccaaatttt gctcgacgat gaccgcagaa acctgttctt tcaacttctt cagaataact
     9841 ccgatgtctt cgatgaccac ggcggctaga ccctgggtgg gctcatcgat cagaaggaat
     9901 ttgggatcgc ccaccagggc ccggccgaca agaaggatct gccgctgccc accgctcagc
     9961 tgaccggctt tgatatgcag aaaattcttc agtttcgggt aaagggccat cactttttcc
    10021 agggcatgat ccatggattc ctggctgcca aaagccgcaa cctcaatatt ttcgcggacc
    10081 gtcagattgc cgaagacctt cttctcctgc cccacatacc tcagccccag aggatagagt
    10141 tcctctggcg atttcccggt aatttcttga tcccgataat ggatggaccc tttctggggc
    10201 ttcagcaggc ccatgatggt cttcaacagg gtggttttcc ccgctccgtt ccgcccgatg
    10261 aaaaatccca tctctccctt ttcgatgcga aaggatacgt catgaagaac cttcgcatgg
    10321 ccgtaagcaa cctccaaatt cttgatttcc agcataagct tttttgctca ccgtaaaggc
    10381 gactcgtgaa tttcccctac agccgggtcc ttcgcttcat agcggttttc cgagcagggg
    10441 ccattcacga accgccctta cgaaagggtc acttcacctt ttccccagta gcactcccgc
    10501 acggaccggt cgctcaagac ctgatccggc accccctcgc agatgaagct cccctcattc
    10561 atcacgcaga gccggtctac gaggttcacg atcttggaaa gcttgtgctc gatgatgatc
    10621 agggtcaggt cttttttgat ccgatggaga atgtccagaa cttccacgat ttccagatcg
    10681 ctcaaacccg acagaggttc atccagaaga agaagtttcg gcctcaggga aagggcgatt
    10741 ccgatctcca gcattctctt ggagccatag gagagctcgg aggtcacggt ttgccaattc
    10801 ttttccagtc cgaccccttt catcgcctca aagcagctct cccgaatgtc ctctcgattg
    10861 gcaggagaga ataaaaagcc ccattccgac agcgattccc ggcggaaacg aaggctggag
    10921 aggaccaggt tctcccaaac cgtcaaggaa ttgaaaaccg agaccaattg gaaggtccgc
    10981 accattccct ggaggactct ctggttcggg gcgaggtccg ttatgtcttt tcggaagaaa
    11041 tgaatctgtc cctgggttgg aggaaataat cctgtgagga gattgaagaa ggtcgtcttt
    11101 ccggacccgt tgggcccgat gatcccaaca gattcattgg gctgaatctg gaaactcacc
    11161 tggttaaccg caacaagacc cccgaaatgt ttgcttaatc cttcagcctt cagaacttcc
    11221 gccatggaaa ggaccttatg aactctaacc ttcggacccc gtaggggcgg gtttcaaacc
    11281 cacccctaca ttccgcattc cgaaatccgc aatcggatca gtatcccatt tctttcagag
    11341 ggagacaaag ctcgggaccg gtgtagacct caaccatttt ggcgatatcc gatctgtcct
    11401 tccgggcttt ggcccctttt ccgtccatga tgtaataaac atatttgtaa tccggccggc
    11461 catcgattcg ccacttcgcg ggaccttttg gtcccatgaa ttccggtttg tccatgatcg
    11521 ccttgtacac tttggccggg tctgtggagt tggacgtctg gaccccacgc agggtttcca
    11581 ttatggcgag gtaagtaaag gtcgcaaaac tatccggcac atccccgaat tcctttaaat
    11641 atttttggac aaattcattg gtcgccttct cggtcgccgg atccttcagc ccgctcaagt
    11701 catggtacca ccaccacccc atggtaacac catccagggc gtcggcgggc actccggatg
    11761 ccagagtgta gccgatcgaa ttaaagacga ttttcgattc ctttctcagc cctaattcat
    11821 acacctgctt cagggcgttg atggcatcgt tgccccagtg gcccatcatg accacgtcgg
    11881 gtttggc
//
LOCUS       DASPRR010000242         6503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_59580, whole genome shotgun sequence.
ACCESSION   DASPRR010000242 DASPRR010000000
VERSION     DASPRR010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59580"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /gene="porA"
                     /locus_tag="VEL68_09425"
     CDS             complement(<1..281)
                     /gene="porA"
                     /locus_tag="VEL68_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HXZ36225.1"
                     /translation="MKKIMVGNHAVSWGVMLARAEVISAYPITPQTTIVEELSVLCAD
                     GRLKARFIPVESEHSAMACCIGSSAAGVRTFTATSSQGLALMHEMLHWAT"
     gene            complement(278..2035)
                     /locus_tag="VEL68_09430"
     CDS             complement(278..2035)
                     /locus_tag="VEL68_09430"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXZ36226.1"
                     /translation="MPKQIPLLREGEMPYQPISLASMEWNKTGSWRYLRPRFENKVPP
                     CNEGCPAGQDIEGALVLIGKGRYLEAWDLFKEENPFPAVCGRVCYHPCESSCNRGDFD
                     EPLSINALERFMADMASKHGRKPSLKRREKRPEKVAIIGSGPAGLTCAYHLVRLGYGV
                     TVYEALPVLGGMLRVGIPEYRLPKKVLGQEIDQVLELGVKVEVNARLGNGLSLDDLKE
                     YQAVFLALGNHKSRSLGISGEETQGVMSGVEFLKDVNLGKKIPLGKRVAVIGGGNTAI
                     DAARAALRLGSKPIILYRRTREEMPAFAGEVHEAEEEGIEISLLTSPAQVISRDGRVT
                     QLECVKNRLGPPDEDGRRRPVEIKGSNYMIEVDQIISAIGEEADLVSLPKKLGIKDNT
                     VPTDERGATKQAGVFAGGDITPQPHTVVHAIASGKRAAIFIDSHLRKQKWEGLFEALR
                     VGEKGSLSMKRYLQEEEERVPLSGRTVLLKDLNLDYFEYQKRLKMPKAKVSRRAGSFE
                     EVNLGFNEEAAVEEARRCFNCGVCNLCDNCYIFCPDVAIQKGKEGELNIIDYDHCKGC
                     GICMEECPRDVIVMEVEGR"
     gene            complement(2132..2680)
                     /locus_tag="VEL68_09435"
     CDS             complement(2132..2680)
                     /locus_tag="VEL68_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ36227.1"
                     /translation="MIEIRIHGRGGQGAVIASEVLASAFFKEGKYVQAFPAFGVERRG
                     APVTAFTRVDEQPIRIRHFIYEPDHIIILDPTLIENTQVDGGLKENGWIVINTDKAPK
                     SFGRFSRFRVATVDANRIAIEHRLGSPTAPIVNTAILGAFAKITGMVAVDSLVEAMAE
                     FVPANKAGNAAAIKEAYEKAIA"
     gene            complement(2791..3375)
                     /locus_tag="VEL68_09440"
     CDS             complement(2791..3375)
                     /locus_tag="VEL68_09440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ36228.1"
                     /translation="MEIEVKRLKLGEKVRELRLKRQNTLRQVAEKTGLSIPLLSQVEN
                     NAVSPPVATLLRIAKALGVGIGHFFREEESKEKAVVVRKHERKKTFRPIHDQKRNSGY
                     TYEALAYTKNSKHMEPFLVEFEPKKKESVTYLKHRGEEFIFLFRGRLAFHYDQQEIIL
                     EPGDSLYFESDLPHGFRSLRGNKAQAIVVVFPEE"
     gene            complement(3523..4746)
                     /locus_tag="VEL68_09445"
     CDS             complement(3523..4746)
                     /locus_tag="VEL68_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ36229.1"
                     /translation="MIAKELKEEVYKCIKCGICLTPCPVYNLLRFEGASPRGRVQLIK
                     KILEGKTELSENFQSLLFTCLLCETCTANCPSGLKTDRLMKTMRSVIQERLGTDRAKK
                     MLGNFLRDRRRPPFFLFCERTRENLISSRKGRSELLADSGIPRPSSKTLRQQVPEVNA
                     APEPRGKVLYFTGCATDYVYPNVGKSVIKVLNRLGMEVILPPGQMCCGLPLFQAGGRK
                     EALKNIRENLKLFNREDVDTVVVDCAACGAALKKEYFLLLEEMGEDAKPAEELAKKVV
                     DISQILVPSDLGGLLKPVPVRVTYHDPCHLGRSQGVREEPRNLLKRVPQLELVEMAGA
                     EACCGGGAALPWEHPEVASGISRNKVQSIRETRATVVASGCPWCRRQIGENLGQDSIR
                     VLHPVELLAEALGDT"
     gene            complement(4743..6128)
                     /locus_tag="VEL68_09450"
     CDS             complement(4743..6128)
                     /locus_tag="VEL68_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ36230.1"
                     /translation="MLSPDVLPKFISIVGPERCKTSPEDLLAYSYDAYVNEFLPDAVL
                     LPKDTAEVAAIMKLASAQSVFVTPRGAGSGLGSGSLAKQGGVVLSFSMMNRILEINTA
                     NRYAVVEPGVVIGEFQRAVEKLNLFYPPDPGSANVATMGGSVVMNAGGMRGVKYGVTR
                     DYVLGLEVVLPSGEVMQTGAITAKDVTGYDLTRLICGSEGTLALVTKVIVRLLPKPKT
                     KRTLQAIYNSLDEAGNTVARIIEAGILPATLELMDKTIIKSLEDFLHLGLPLDAAAVL
                     LIDVDGEPETVEKLANLVADFCKGQGARDVRVSQSEKENEQLWVARRAAFGAIARVRP
                     TCIIEDATVPVSLLASAIKKILEVGERNRVQIAVLAHAGDGNLHPFFLCDHRDKEEMA
                     RVEKAMDELVDYAMSVGGTLSGEHGIGIAKSKYLPRQLSPASLSLMRGIKKTFDPQGI
                     LNPGSFLEHRA"
BASE COUNT         1275 a         2019 c         1772 g         1437 t
ORIGIN      
        1 gtggcccagt ggagcatctc gtgcatcaac gccagcccct gggaggaggt ggcagtgaat
       61 gtccggaccc ccgccgccga agacccgatg cagcaggcca tggcagagtg ctcggattcc
      121 accgggatga acctggcctt aagccttccg tcggcgcaca ggactgaaag ctcctccacg
      181 atcgtggtct ggggggtaat cggataggcg gagatcacct ccgcccgggc cagcatgacc
      241 ccccaggaga ccgcgtgatt gcccaccatg atcttcttca tcttccctcc acctccatga
      301 cgatcacatc ccgggggcac tcttccatgc agattccaca gcccttgcaa tggtcataat
      361 cgatgatgtt cagctcccct tctttccctt tttggatggc cacgtccggg cagaagatat
      421 agcagttgtc gcatagattg cagaccccgc agttgaagca gcgccgggcc tcctcgacgg
      481 ccgcctcttc gttgaagccc aggttgactt cttcaaagga ccccgcgcgg cgggacacct
      541 tggccttggg catcttgagg cgcttctggt attcgaagta atccaggttc aggtctttca
      601 ggagaaccgt cctgccgctc aagggaaccc gttcctcctc ttcttggagg taccgtttca
      661 tggacaggct tcccttttcg ccgactcgaa gcgcctcgaa gagaccttcc cacttctgtt
      721 ttctcaggtg cgagtcgatg aagatcgccg cccgctttcc cgaggcgatg gcgtggacca
      781 ccgtgtgggg ttgcggggtg atatctcccc cagcgaagac ccccgcctgt ttggtcgccc
      841 cccgctcgtc cgtgggaacc gtgttgtcct tgatccccag tttcttcggc agagaaacaa
      901 ggtcggcctc ttctccgatg gccgagatga tctggtctac ttcaatcata tagttggaac
      961 ccttgatctc cacgggccgc ctgcggccgt cctcatccgg cgggccaagg cgattcttca
     1021 cgcattccag ctgggtgacc ctcccatcgc gagagatcac ctgggccggg gaggtgagca
     1081 gggagatttc aatcccctct tcttcggcct cgtgaacctc cccggcgaag gctggcatct
     1141 cctcccgggt ccggcggtag aggattatgg gtttggaccc tagacgcagg gctgcgcggg
     1201 ccgcatcgat cgccgtgttt cccccgccga tcaccgccac ccgttttccc agggggattt
     1261 tcttccccag gttcacgtcc ttgaggaatt cgaccccact catgactccc tgggtttctt
     1321 ctccagaaat ccccaggctc ctgctcttgt ggttccccag ggccagaaag acggcctggt
     1381 attctttcaa gtcgtccaga gatagaccgt ttcccaagcg ggcgttgact tccactttga
     1441 ctcccagttc caaaacctgg tcgatttcct gccccagcac tttcttcggg agccgatact
     1501 ccggaatccc cacccggagc atccccccca gcaccggcag ggcttcatag accgtcactc
     1561 cgtatcccag ccgcaccagg tggtaggcgc aggtcagacc ggccggacct gaaccgatga
     1621 tggccacttt ttccggccgt ttctcccttc ttttcagcga gggcttccgg ccatgtttgg
     1681 acgccatgtc ggccatgaac cgttccaggg cattgatgga gaggggctcg tcgaaatctc
     1741 cccggttgca ggaggattcg cagggatggt aacagacccg cccgcagacc gccgggaaag
     1801 gattttcttc tttgaacagg tcccaggctt ccaaatatct tcccttcccg atcaggacca
     1861 aggccccttc gatgtcctgg ccggcggggc acccttcatt gcagggagga accttatttt
     1921 cgaaccgggg acggagataa cgccagctgc cggtcttgtt ccactccatg gaagcaaggg
     1981 agatgggctg atagggcatc tccccttcac gcagcaaagg aatctgtttg ggcatagata
     2041 ctccttcaag ctttcagcct tcagctatca gcgttcagcc ttgatttttt cagtttggct
     2101 gattgctgag cgctgacggc ttatcgctgg gttaggctat ggccttctca tacgcctctt
     2161 tgatcgccgc ggcattgccc gctttattgg ccgggacgaa ttcggccatg gcttccacca
     2221 gcgaatctac cgcgaccatc ccggtgattt tggcgaatgc cccaaggatg gccgtgttga
     2281 cgatgggggc cgtcggagag cccagccggt gctcgatggc aatccggttg gcgtccaccg
     2341 tggccacccg gaaccgggag aaacgcccga aagatttggg ggccttatcc gtattgatga
     2401 caatccaccc gttttccttc agtcctccat ccacctgggt gttctcgatc aaagtggggt
     2461 cgaggataat gatatggtcc ggctcgtaga taaagtggcg gatgcggatc ggctgttcgt
     2521 ccacccgggt gaaggccgtg actggagccc cccggcgctc taccccgaag gcagggaagg
     2581 cctgcacgta tttcccttct ttaaaaaagg ccgaggccaa aacctctgaa gcgattaccg
     2641 ccccctgccc tccccgacca tggatgcgga tttcgatcat gtcaaactcc cttcaaaagc
     2701 agcgctcagc tttcagctat cagcggtcag cttaaacaaa taaatgattt ttgctgatcg
     2761 ctggaggctg acggctgatc gctgtctttc tcattcttcc ggaaacacca ccacaatggc
     2821 ctgggcctta ttgccccgca gggaccgaaa accgtggggc aaatcggatt caaaatacag
     2881 gctgtccccc ggctccagaa tgatctcctg ctggtcgtaa tgaaaagcca gacgaccccg
     2941 gaaaagaaag ataaactctt ctccccggtg cttcaggtag gtaaccgact ctttcttctt
     3001 gggctcgaat tccaccagga agggctccat gtgtttcgag ttcttcgtgt aggccagggc
     3061 ttcataggtg tatccgctgt tccgcttttg atcatggatg ggccggaagg tcttcttgcg
     3121 ctcgtgcttg cgcaccacca cagccttttc tttcgattct tcctcccgga aaaagtgccc
     3181 gatgccgacc cccagggctt tggcaatccg cagcagggtg gccaccgggg gagagacggc
     3241 attgttctcc acctgggaaa ggaggggaat ggagagacct gtcttctcgg ccacttggcg
     3301 aagggtattc tgcctcttca ggcgcaactc ccgcaccttt tccccgagct ttaatctttt
     3361 gacttcaatc tccacgttca tcctgtcggc ctaaaaaaag cgcatagtgc aaagagcaca
     3421 gagaatagaa aaaaaatgac gatgaactgc aatccatcct gatcctttaa ccagcaacaa
     3481 gcaaccagca acgttctttc agtcttcatt ccgaattccg cattaggtat ccccaagggc
     3541 ctcggcaagc aactccaccg gatgaagaac ccggatcgaa tcctggccca ggttctcccc
     3601 aatctgccgt cggcaccagg ggcaacccga ggccaccacg gtggcgcggg tttccctgat
     3661 ggactgaacc ttgttccggc tgatgccgga ggccacctcg ggatgctccc atggtaaggc
     3721 tgccccgccg ccgcagcatg cctcggcacc cgccatttcc accagctcca gttggggaac
     3781 ccgcttgagc aggttgcggg gttcctccct gaccccctgg cttcgcccca gatggcaggg
     3841 atcgtggtag gtcacccgaa ccggaacggg cttcagtaac ccccccaggt ccgaggggac
     3901 caggatttgg gagatgtcca ccaccttctt ggccagctct tccgccggct tcgcatcctc
     3961 tcccatctcc tccaggagga ggaaatactc tttcttcagg gcggccccgc aggcggcgca
     4021 atccaccaca accgtatcca catcctcgcg gttgaagagc ttcaggttct cgcggatatt
     4081 ctttaaggcc tcctttcgcc ctccggcctg gaagagcggt aagccgcagc acatctgccc
     4141 cggggggaga ataacttcca ttcccaaccg attcaggacc ttgatcaccg atttcccgac
     4201 gttagggtaa acatagtccg tcgcgcaacc ggtgaaatag agaactttcc ccctcggttc
     4261 cggcgccgcg ttgacctcgg ggacctgctg gcgcagagtt ttggaacttg gccggggaat
     4321 tccggagtcc gcgagaagtt ctgaccttcc tttccgggag gaaatgagat tttcccgggt
     4381 gcgctcgcag aacaggaaga agggcggacg gcgcctatcg cgaaggaagt tccccaacat
     4441 ttttttcgcc cggtcggtcc ccagccgttc ctggatcacc gatcgcatgg ttttcatcaa
     4501 ccggtccgtt ttcagtccgc tgggacaatt ggccgtgcag gtttcgcaga gcaggcaggt
     4561 aaagaggagg ctttgaaaat tctcggaaag ctccgtcttc ccctccagaa tcttcttgat
     4621 caactgcacc cgtccccgcg gagaggcccc ttcgaatcga agcaggttgt acaccgggca
     4681 gggcgtcagg caaatcccgc acttgatgca cttgtagact tcttctttca attccttggc
     4741 gatcatgcgc gatgctccag aaaactcccc ggattcagaa tgccctgggg gtcgaaggtt
     4801 tttttgatgc cccgcatgag actcagactg gccgggctga gctgccgggg aagatatttc
     4861 gatttggcga tcccgatccc gtgctccccg gagagcgtcc cgcccacgga catggcgtag
     4921 tccaccagct catccatggc cttctccacc ctggccattt cctccttgtc tctatggtcg
     4981 cagaggaaga aggggtgaag gttcccgtcc ccggcatggg ccaggacggc aatctggacc
     5041 cggttccgct cgcccacctc caggatcttc ttaatggccg aagccaggag gctcaccgga
     5101 acggtggcat cctcgatgat gcacgtgggc cgtacccggg cgatggcccc gaaggcggcc
     5161 cgccgggcga cccacagttg ttcgttttcc ttctccgact gggagacgcg aacatcccgg
     5221 gccccctggc ccttgcagaa atcggccacc aggttggcca gtttttccac cgtttccggc
     5281 tctccgtcca cgtcgatcag cagaactgcc gcggcatcca ggggcaggcc caggtggagg
     5341 aaatcctcca gggacttgat aatggtctta tccatgagtt ccagggtggc gggaaggatg
     5401 cccgcttcga tgatccgggc caccgtgttt cccgcctcgt ccaggctgtt gtaaatggcc
     5461 tgaagcgtcc gcttggtctt cggtttgggc aggaggcgca cgatgacttt ggtcaccagc
     5521 gccagagtac cctcggaccc gcaaatcagg cgagtgagat cgtaaccggt cacatctttc
     5581 gcggtaatgg cgccggtctg catcacctcc cccgagggga gaacgacctc cagccccagg
     5641 acataatccc gggtgacccc atatttgacc ccccgcattc ccccggcatt catcaccacc
     5701 gatcctccca tggtggccac gttggcactg cccgggtccg gaggatagaa gaggttcaat
     5761 ttttccactg ccctctggaa ttcgccgatg accacccccg gctccacgac ggcataccgg
     5821 ttggccgtgt tgatctccag gatgcggttc atcatggaaa agctgagtac gacccctccc
     5881 tgtttggcca gcgaacccga tcccagaccc gacccggcgc cccggggggt gacaaagacg
     5941 ctttgggctg aggccaactt catgatcgcc gcgacttcgg cggtgtcttt cggcaggagc
     6001 accgcatcgg gaagaaattc attgacgtag gcgtcgtagg aatacgccag gaggtcttcc
     6061 ggggaagtct tgcaccgctc cgggccgacg atggatatga atttcggtag aacatccgga
     6121 ctcagcatgg caaaatcctt tccggccctg ccccgcccga gttaggacgg gatgtctcgt
     6181 gatttcttgg aaagcctttt ggttggagag aatcttcgga gagatagatt ccgggaggaa
     6241 aagatggagg agaaagaacg atgttgaatc cgcagaagaa taacgcccgc gacatcttcc
     6301 ctcctccccg ccgggggagc agaaagagaa attacggccg gcctttttgc agtcacctta
     6361 ccatattaca tcccatcttc cctgtcaaga aaattttagc ctgatgaaaa ttaatttact
     6421 attaccaaat attaacttcc ccggctcgtt gcgggttgcc agttcctggt tactggttgc
     6481 tggttaccca ttaaaggaaa taa
//
LOCUS       DASPRR010000243        19427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1832357, whole genome shotgun sequence.
ACCESSION   DASPRR010000243 DASPRR010000000
VERSION     DASPRR010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19427)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19427
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832357"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1121
                     /locus_tag="VEL68_09455"
     CDS             171..1121
                     /locus_tag="VEL68_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ36231.1"
                     /translation="MKKVLCLLAILGCFLVFCSSETFSQTYDLKLMTGPMGGAWYPLG
                     GAISDAIQKAIPGVTLSVMPGGGIGNVEALEVGKCDIGFANSCSAVDGLFGRAPFKKK
                     MENMRQLANLYPQYFQMAVAEDSGIKSVADLKGKVLACDRKGLTGEQLSELVLKVYGL
                     SYKDLAKVNHVSYSDGVSLMKDGHVQAFFLITTIPSSSIIDLSADRKIRMLSLPEDKI
                     QALQKINAGFLKRVIPKGTYPGVDYEVNGVGAFTHLIISSKLPDDLVYQITKVIAVNL
                     SRFGDVVKDIKGVTPKDLAFDVGVPYHPGALKYYKEIGAL"
     gene            1203..3137
                     /locus_tag="VEL68_09460"
     CDS             1203..3137
                     /locus_tag="VEL68_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HXZ36232.1"
                     /translation="MDAEHFQVKDKETDFQTETLPGAIKKIISVVAIAMSVYHLYTGY
                     FGAPEALLHRAIHLLFTMVLILFLFPLTKKEWGRNLRWTDGILILLVVASIGYLFLNY
                     EYVVTRYALVHPLEKLDFVFGILLSLLLLEASRRAIGPALPITAIVFLVYAYLGPYLP
                     GLLRHTGFSTEMIVDQLYLTTEGIFGMPLGVSATYVILFIIFGTFLEKTGTGQLFMEF
                     ASAITGWTRGGPGKISCISSALFGTISGSAVANVMVDGWLTIPLMKRTGFNPPFACAV
                     EATASTGGQIMPPVMGAAAFVMSEFTGIPYITICLYAMIPALLYYLSLFMSIHFMAGQ
                     MGLKGLSRKELPSLKKILLSRGHMFIPLGVIVYMMIAGYTPMYACIYSTVAVVILSFI
                     RKETRMNFRTFLAALEDSAKNTLGVAAACACAGIVIGVINLTGVGLKFTSFVLFIAGD
                     SLIPALILTMIAGIVLGMGMPTTPAYIVQAALLIPALIKLGVLNVAAHMFVFYFSTIS
                     AITPPVAMAVYAAAGIGGAALWPTGLWAMRIAATGFVVPFMFVYGPSLLFIGSAFDIT
                     TSAISATFGVIALAAGMMGWFVKRVSLWERLVLIIAAFLLIKPGLATDAVGYVLLLFI
                     YIRQKYFLKDLPTMGSSGPA"
     gene            3264..4922
                     /gene="ilvD"
                     /locus_tag="VEL68_09465"
     CDS             3264..4922
                     /gene="ilvD"
                     /locus_tag="VEL68_09465"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXZ36233.1"
                     /translation="MEKRSDILHRGDARAANRALLRSLGLTDKDFAKPFIGIVNTWTE
                     INPGHIHLRTLAEAAKSGVRMAGGIPFEVNTIALCDGIAQGHAGMRRILPSRNLIADS
                     IELVVDANQFDAFIVLASCDKIVPASWMAIARLDLPALMITGGPMMPGRFRGEQLVLP
                     AAREAQGRMLRGEITQADFQEIEECLCPGPGSCSMMGTANSTSCLTEVMGLSLPGCGT
                     SHAVDAKKLRIARESGEQILRIWKEGLKPSKILTLSALKNAVTASMAFGGSTNIVLHL
                     MALANELNIPLSLETFDEISRRTPFICSIKPSGKYPMLGLEDAGGVPAVLHELRDLLD
                     LKVLTVSGKTLEENLSGVQNRNPEVILSRQKPLRKEGGIAILRGSLAPGGAVVKQSAV
                     SEKIMVHTGPARVFETEVSAREAVFGNRVKPGEVIVIRYEGPKGGPGMPEMLSVTAAL
                     VGMGLAESVSLVTDGRFSGSTRGPCIGHIVPEAQDGGPIALVRDGDSIAIDIPNRRLD
                     LRVPPEELERRRKAWTPPPLKVNRGYLKTFAERVSSADKGCISI"
     gene            5056..6456
                     /locus_tag="VEL68_09470"
     CDS             5056..6456
                     /locus_tag="VEL68_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ36234.1"
                     /translation="MLDRVVIREISTIVGKENVVEDRETRVCYSYDATNIKHLPDLVV
                     FARTPQHISEILKLANENRFPVIPRGAGTGFTGGTIPVEGGVVLVLTKMNRILKIDQE
                     NLLAIVEPGVVTYDLQQEVEKIGLFYPPDPASLKSSTLGGNVAECAGGPRAVKYGVTK
                     DYVLGLEAVLPTGEIISAGVQTVKSVVGYDLTKLLVGSEGTLGVITKIILRLLPLPKA
                     KRTMLVIFPTIESAATSVSQIISSRIIPTTLEFMDNSTIRCVEDYLHMGLPVDAGALL
                     IIEVDGAPEALNGEIEEIQKICQTNRAMEIKVAKDKQEAEELWKARRAVSAAVVKLNP
                     TKINEDVTVPRSKVADILRRTEAISKKHGLIIVNFGHAGDGNIHVNVLIDKRKPGEQE
                     KAHAAVKEIFQAALDLGGTLSGEHGIGITKAPYLPLELGDMGIEVMKRIKKAFDPNNI
                     LNPGKIFPDGAKATVH"
     gene            6717..8015
                     /locus_tag="VEL68_09475"
     CDS             6717..8015
                     /locus_tag="VEL68_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ36235.1"
                     /translation="MELLGLTTMKEITEVQEEARRCVKCGNCLAQCPVYAETLEEPLA
                     ARGKMALVESLKEDDPEYTNRYYKILSQCLLCGTCAENCPNGVCGDEIIREARALLVE
                     KKGLTLPKKAIYKYFLDTDRLMPLLLKAGASLQHFFLEKIPEESGLRLRFPMPFIDSK
                     RFIPSLAPDPFLDIRSGWVRAEKETMRVGLFVGCVSNYMFPRIAQSTLDLFVRNGISV
                     YIPKEQKCCGLPAFGSGDDETPRSIARRNIKAFAEENLDQVISHCASCAAMLKLDYPL
                     LFGEDDPFRRKALAFSEKVSDATLFLSKVMEPPATDRRPPTKSVRVTYHDPCHLRRKL
                     GIFKEPRALLQSTPGLEYVEMKDANRCCGQGGSFNVANYDLSLKILDRKTKAIAESEA
                     DVVTTTCSGCMLQLMDGIHQAGLKKEVRHLVEMVNQATLL"
     gene            complement(8033..8908)
                     /locus_tag="VEL68_09480"
     CDS             complement(8033..8908)
                     /locus_tag="VEL68_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HXZ36236.1"
                     /translation="MFRQKWEGQKMDVNAMNERVTGVLKSRKLKIIVGGIVLLLILLV
                     ANPFLTIPAGHRGVILNWGAVQKDVLPEGLAFRWPVYQQVVRADVRILVTQPDCDAVT
                     KDQQVNHTKVAVNYHVIPSEASWIFQNVGIDYKDKLVSPKIQEIVKAITARYTALELI
                     SKREQIRNEMKDHLKEALHPYKIVVDDFSVVNFKWDVEFQKAIEAKQVAEQLAMKAQR
                     DLERIKIEAEQKITQAKAEAESLRLQKENVTPYLIKLRQIEANIKAIEKWNGQLPRFS
                     GGAVPFIDMKSLDEK"
     gene            9155..10702
                     /locus_tag="VEL68_09485"
     CDS             9155..10702
                     /locus_tag="VEL68_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36237.1"
                     /translation="MRLGRFLILFCLGFLFILFAFSPALAGKDHLIVAFEDSVKTMDY
                     YQTTQRIGIITGYMMYDPLLKRDPKDGSLHPHLVTEWKAINDTTWEFKLRPGVKFHNG
                     NPLNAEAIRFTIEDRILDPAQKSPTAAGWKWVKKVEVKDDLTFRIITDGPYPLILERF
                     NVLFPYDPKWVKEMVAKNGEVYLARNTMGTGPFKFVKFIEGDRIELVRNENYWEKGYP
                     KFPKMTIRFIPEQSTRIAELISGGIDAALNILSDQIPTIEKSKNSETIIVPILRVSFW
                     QFDSSGRAPNSPPALKDVRVRRAIWHAIDREAIIKNVLGGNADLVNIPMSPKQFAADL
                     SIKGYEYNPEKAKVLLKESGYEKGFNLNVWTYTAPCKMANEAAIGYLENVGIKGVIRD
                     YVGRVGEMEKLCIASKTDGALNMTWGSYNIFDPDAIMTYFFMSPEAPYDYTNDAELIG
                     WLREARTTLDKQKRKDLYFKAQKRIIDQAYWMPFFTQRYIAGVNKHLGLTVGIDEVPR
                     FQYATWK"
     gene            10883..11803
                     /locus_tag="VEL68_09490"
     CDS             10883..11803
                     /locus_tag="VEL68_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36238.1"
                     /translation="MRTFIIKQLLQAVGVCLVISMISFFLLFLNTDPALLLLPPEAEV
                     QDIAKFKKQMGLDRPVPVQYLDFLRKVVLHGDFGNSFMAKVPAAKLVRERLPATVELA
                     LAALILVNLVALPVGIISAIRRYSLLDNVATFTALLGQAMPLYWFGIMLIIIFGVWLR
                     WFPISGSDTWAHLVLPAITLASWILPINMRLVRSGMLDVLNQDYIRTARAKGLVERKV
                     LIKHAFKNAAIPVITITGMQLGALLGGAVVTETVFAWPGLGRLAVDSIRMGDYPVVQA
                     IVVVFALFVVIGNFAADILAALIDPRIRLE"
     gene            11808..12713
                     /locus_tag="VEL68_09495"
     CDS             11808..12713
                     /locus_tag="VEL68_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36239.1"
                     /translation="MAEIPTSNPEIDEAAPSILSLVLRKMWHLRMGVLGALLIFCLIF
                     IALFCPYVSPHDPFKQDITKRLWKPAFMEGGKAEYLLGTDHLGRDLLTRILYGTRISL
                     VVGLAAVILMLTVGVTLGLVAGYFGGKADAVISFLVNCMMGFPFILLAMSLVAVLGAS
                     LQNIIIALGLTGWPVFTRVTRIETMKLRDREFVLAAKSLAFSTRRILVRHILPNLLPA
                     ILVLATVEVARAIIRESLLSFLGLGIQPPTPSWGVMLAEGRDYMLLQWWLAAFPGMAI
                     FMSALGINLLGDALRDLVDPYLRKT"
     gene            12713..13762
                     /locus_tag="VEL68_09500"
     CDS             12713..13762
                     /locus_tag="VEL68_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ36240.1"
                     /translation="MDRPAPESRLTSNSISATESGDDFLLRVQGLKKYFPIRKGLLGR
                     TVGYVYAVDGVSFAIRRGETLGLVGESGCGKSTIGRCLLRLYEATDGQIFFQGMDISK
                     LSPGEMIQFRTRIQMIFQDPYSSLNPRLRVREIIGESLRVHNLVPQGDWQDRVRELLA
                     EVGLQPDHMARYPHEFSGGQRQRIGVARSLAMNPDLIVADEPVSALDVSIQAQVINLL
                     DSLKDKHGLSYIVIAHDLAVVEHISDRIAVMYLGKLVETARDRDLYLNPKHPYTEALL
                     SAVPIPAPGGRERRKSRKLIQGDVASPINPPSGCRFHTRCLKTMEECRRIDPPLIEIG
                     PEHHVACLLYGGGRP"
     gene            13759..14757
                     /locus_tag="VEL68_09505"
     CDS             13759..14757
                     /locus_tag="VEL68_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36241.1"
                     /translation="MRESLIQLKNLTTHFFTPEGVVKAVERVTFGIDKGRTLGVLGES
                     GCGKSVTALTIMRLIPDPPGKILEGEITFEGSDLLQLSPGDMRQVRGRDIAMIFQEPM
                     TSLNPVYTIGDQIAETYVIHQRMGKPEAFQRAAEMLRLVGIPAPERRVHEYPHQLSGG
                     MRQRAMIAMAMSCRPKLLIADEPTTALDVTIQAQILDLMLGLQEEVGMAIMIITHDLG
                     VIAEVSDDVVVMYAGQVVECASIETLFLQPRHPYTLGLMSSIPQLGRKFKEGKARLKE
                     IPGLVPNLIRLPPGCLFQPRCPMAFHTCTQQRPPLFSLDSGHGVRCWLVEKESKNP"
     gene            complement(14809..15558)
                     /locus_tag="VEL68_09510"
     CDS             complement(14809..15558)
                     /locus_tag="VEL68_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HXZ36242.1"
                     /translation="MKIDTQKYQKFHDRLGLKEHPLAAFYSNHKPGAGLSPKEKGHLC
                     MFALLKRARHEGDTVYFDADHVGCFGGGYYMGFQSTPRPNIEYFLSCGIPGEMEGERY
                     IKTPEIARKYFASTQPRKAPAKYCLFKPLDKLTNAEEPEVVIFFASPDVVSGLFTLTN
                     YAAERMDAVRIPFSSGCGAILSHPLKEAEKKNPQAILGLFDVSARPFVEPHILTLAMP
                     TGLFLSLLDNQEESFLITKSWEMVRKRIEKA"
     gene            complement(15668..16276)
                     /locus_tag="VEL68_09515"
     CDS             complement(15668..16276)
                     /locus_tag="VEL68_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXZ36243.1"
                     /translation="MINRTHRRSLRLPNYDYAQAGAYFVTVCTNGRKCLLGEIVGGEM
                     RLSDAGRMVESVWIDIARHYSSVDIDAFVTMPNHIHGIIILGVGAGPRACPDEKKRMG
                     QPQRVAPTISLPEVVQRFKSLTTTKYIKGVAQESWPAFPGRLWQRNYYEHVIRKENEL
                     TLIREYIQLNPTKWAVDKENPGAIQTVPAKREEIENILGGRP"
     gene            complement(16404..17504)
                     /locus_tag="VEL68_09520"
     CDS             complement(16404..17504)
                     /locus_tag="VEL68_09520"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXZ36244.1"
                     /translation="MSSNAKIKRIGILTAGGDCPGLNAVIRAIVKTAIYRYGLEVVGF
                     IDGYSGVIHNKARILESKDASGILPRGGTILGTSNRDDPFRFPVVLNGQKTFKDVSNQ
                     SVKNLRKNKIDVLFVLGGDGSLTIGNKLSQKGVKIIGVPKTIDNDLWGTDVTFGFDTA
                     LQVAMGAVDRLHTTAESHHRVMVVEVMGRYAGWIALMAGVAGGGDVILIPEIPFDYEK
                     ISEQIKERRRLGKRFSIVVVAEGAKPKKGEVVVQRVIEDSTDPLRLGGIGYRVGNEIE
                     KRTGVETRVTVLGHVQRGGSPSAFDRILATRFGARAIELAMEGKFGRMVCLRGTQIKD
                     IPLEKVGGRQRRVRPDGEMVRTARSIGVIFGD"
     gene            complement(17672..19303)
                     /locus_tag="VEL68_09525"
     CDS             complement(17672..19303)
                     /locus_tag="VEL68_09525"
                     /inference="COORDINATES: protein motif:HMM:NF006122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ36245.1"
                     /translation="MPRMTGGEAVVEALKREGVEYLFSIPGVQIMSVYDALYGEKDLR
                     LVVVRHEQTALYMADGYARVQGRPGVGLVVPGPGVQNTLAALGTAYACSSPVLLVAGQ
                     VETKDLGRDGGALHEVNDQLDMVRPVTKWCKRVMRVEEIPDAIREAMRQMATGRPRPT
                     EVEVPWDTMRSSGEVEFPPPQIQPPKDPDQEVLRRAADLLARAQKPLIWAGGGAIVSD
                     SSPEMKDLAEALGAPVATTAQGKGVIPENHPLSLGGAYYGFGPVRWAMPEADVVLTVG
                     TRITWQQARPATALRPPQKLIQLDADPSMIGMNYPAEVAIVADAKAALRALVEEARKR
                     KVLKERWASAELDQFRKKHHQWLQEKAPLQLGILKAVREELADDSIFVCGVTNIGYWA
                     NLAYEVRRPRTFITSSYFATLGYSFPTALGAKMAAPDRQVVCVVGDGGFLYACAELAT
                     AVRYGINLVTLVFNDQAFGSTKSDQMVNFQGRVVGTEVNNPDFARLAEAFGAKGLKAT
                     PEQLGKVLREALGLKKPVVIEVPLPTMVAPFQILE"
BASE COUNT         4094 a         5766 c         5168 g         4399 t
ORIGIN      
        1 cggccgctcg cccctacaac ggtcaaactt ctgtggcacg cggccgcgcc ctcctacaaa
       61 ctatacacgg cgtgctaatt cgaacccctg gcggcagggg taaatgccaa acgcccaaat
      121 catagattgt tcagacttgc cagattcatc actcgattga ggaggaaaat atgaaaaagg
      181 tgctctgcct tttggccatc ctgggttgct tcctggtctt ctgctcttcg gaaacctttt
      241 cccagacata tgacctaaag ctcatgaccg gtcccatggg aggggcttgg tacccgctcg
      301 gaggggcaat ctccgatgcc atccaaaagg cgatccccgg agtgaccctg tcggtcatgc
      361 ccggcggggg aatcgggaac gtggaggctc tggaagtggg gaaatgtgat atcggcttcg
      421 ccaattcctg ctcggccgtg gacggcctct ttggccgggc tccctttaag aaaaagatgg
      481 aaaacatgag acagttggcc aacctatatc cccagtactt tcagatggcc gtggcggaag
      541 attcggggat taaatcggtg gccgatctga agggcaaggt cctggcttgc gaccgcaaag
      601 ggctcaccgg agagcagttg tcggaactgg tcctgaaggt ctacggcctg tcctacaaag
      661 acttggccaa ggtcaatcat gtgagttaca gcgacggcgt atccctgatg aaggacggcc
      721 acgtccaggc cttctttttg atcaccacca tcccttcgtc ctccatcatc gacttgagcg
      781 ccgaccggaa gatccgcatg ctctcccttc ccgaagacaa aattcaagcc ctgcagaaga
      841 ttaacgccgg cttcctgaag agggtcattc caaaagggac ctatcccggc gtggactacg
      901 aagtcaacgg cgtcggcgcc ttcacccacc tcatcatcag ctccaagctt ccggatgacc
      961 tggtatatca gatcaccaag gtcatcgcgg ttaacctttc ccgctttgga gatgtagtca
     1021 aggacattaa gggcgtaacc cccaaagacc tggccttcga cgtgggggtc ccctaccatc
     1081 ccggcgcttt gaagtactac aaagaaatcg gagctctgta agagtcccat ctgaacaggt
     1141 tacaggagag gccgggagaa ctcttctccc ggcctaaaaa agaatgccgt aaggagcagg
     1201 ccatggatgc tgagcatttc caggtgaagg acaaggaaac cgatttccag accgaaacgc
     1261 tgccgggggc gatcaagaag atcattagcg tcgtcgccat tgccatgagc gtttaccatc
     1321 tctacacggg ctatttcggg gcccctgaag ccctccttca ccgtgccatt catttgctct
     1381 ttaccatggt tttgattctt ttcctcttcc ccctaaccaa gaaggaatgg ggaaggaatc
     1441 ttcgctggac cgatgggatt ctaatcctgc tggtcgtcgc ttccatcggg tacctatttc
     1501 ttaattacga gtatgtggtc acccgctatg cgctggtcca tcctcttgag aaactggatt
     1561 ttgtcttcgg aattctcctg tccctgcttc ttctggaagc ctctcgccgg gccatcgggc
     1621 cggccctccc catcaccgca attgtattcc tggtctacgc ctatctagga ccctatctcc
     1681 cgggactcct ccgtcatacc ggattcagca ccgaaatgat cgtggatcag ctttacctga
     1741 ccaccgaggg aattttcggc atgcctctgg gagtctcggc tacctatgta attctcttca
     1801 tcatcttcgg aacgttcctg gaaaagaccg gaaccggcca gctcttcatg gaatttgctt
     1861 cggccattac cgggtggacc cggggggggc cgggcaaaat ttcctgcatc tccagcgccc
     1921 tcttcggaac catctccgga agcgctgtgg ccaatgttat ggtcgatggg tggctgacca
     1981 ttcccctgat gaaacggacc gggttcaacc ctccctttgc ctgcgccgtg gaggccacgg
     2041 cgtccacagg cggccagatc atgcccccgg tcatgggagc ggcggccttc gtcatgtccg
     2101 aattcaccgg aattccttat attacgatct gcctttacgc gatgatcccg gctctcctct
     2161 attatctttc ccttttcatg tctatccact tcatggccgg gcaaatggga ctcaaggggc
     2221 tttcccggaa agaactcccc agcctgaaaa aaatcctgct ttcccgggga cacatgttca
     2281 ttcccctggg agtcattgtt tatatgatga tcgccgggta tacccccatg tacgcctgta
     2341 tctattccac ggtggcggtg gtgatcttat cctttattcg gaaggaaacc cgcatgaact
     2401 tccggacttt tctggcggcc ctggaagata gcgccaagaa caccctgggg gtggccgcgg
     2461 cctgcgcctg cgcgggaatc gtcatcggag tgatcaacct gaccggcgtg ggactgaagt
     2521 tcaccagctt tgttcttttc atcgccgggg actccttgat tccggccctt atcttgacca
     2581 tgatcgccgg aatcgtcctg ggcatgggca tgcccaccac tccagcctac atcgtccagg
     2641 ctgccctgct gatcccggcg ctcatcaagc tgggagtcct gaatgtggcc gcccacatgt
     2701 ttgtttttta tttctccacc atctccgcca tcacccctcc ggtggccatg gccgtgtatg
     2761 ctgccgcggg aatcggggga gcggccctat ggcccaccgg gctttgggcc atgcgcattg
     2821 cagccacagg ctttgtcgtt ccctttatgt tcgtctacgg gccatcgctt ctcttcatcg
     2881 gttcagcctt cgacatcacc acctcggcca tcagcgccac cttcggagtc atcgccttgg
     2941 ctgccggcat gatggggtgg ttcgtcaaaa gggtgagcct ctgggaacgg cttgtcctga
     3001 tcatcgccgc atttcttctc atcaaacccg gcctcgccac cgacgcggtg ggctatgttc
     3061 tccttctttt catctacatc cggcagaaat atttcttgaa agatctgcca acgatgggct
     3121 cgtccgggcc cgcctagaaa gctcgaactg aaatccccct tggcggacaa aatcaaaaaa
     3181 ttatgctatc ctattttgat atggaacgag ccgtagcgcg attcatgaat cgtccctgcg
     3241 taggaaattt gcgaggttag gaaatggaaa aacgtagcga tatactccac cggggagacg
     3301 cccgggccgc caaccgtgcc ctcctgcgtt ctctcggact gacggacaag gatttcgcaa
     3361 agcctttcat cggaatcgtc aacacctgga cggagattaa cccgggccat attcatttaa
     3421 gaactctagc cgaagcggcc aaatccgggg tccgcatggc cggagggatt cctttcgaag
     3481 tcaacaccat cgccctctgc gacggcatcg cccaggggca cgcgggaatg aggcgtatcc
     3541 tccccagccg gaacctcatt gccgattcca tcgagctggt cgtggacgcc aaccagtttg
     3601 atgccttcat cgtcctggct tcctgcgaca agatcgtccc ggcgagctgg atggccatcg
     3661 cccgcctgga tttgccggct ctcatgatca ccggcgggcc catgatgccc ggccgtttcc
     3721 ggggagagca attggttctt cccgctgccc gggaggctca ggggaggatg ctccgggggg
     3781 agatcaccca agcggatttt caggagatcg aggagtgcct gtgccccggg cctgggtctt
     3841 gctcgatgat gggcacggcc aactcgacga gctgtctgac cgaagtcatg gggttgtccc
     3901 tccccggttg cgggacctcc catgcggtgg atgccaagaa actgcgtatt gcccgggaga
     3961 gcggagagca aatcctgcgc atctggaagg aaggcctcaa gccctcgaaa atccttaccc
     4021 tctccgcact gaaaaacgct gttaccgcca gcatggcctt cggggggtcc accaacatcg
     4081 tcctccatct catggccctg gccaatgaac tgaatattcc tctctccctg gaaacttttg
     4141 acgaaatcag ccgccgcacg cctttcatct gcagcatcaa gcccagcggg aagtacccca
     4201 tgctgggcct tgaggatgcc ggcggtgtgc ctgccgtatt gcatgagctc cgggacctcc
     4261 tggacctcaa ggtactcacg gtgagcggga aaacccttga agagaatcta tccggggtcc
     4321 agaacagaaa ccccgaagtc attctttccc ggcagaagcc tttgaggaag gaagggggaa
     4381 tcgccatcct tcgagggtcc ctcgctcctg gtggcgcggt cgtcaaacag tcggcggttt
     4441 ccgaaaagat catggtacat acgggtccgg cccgggtctt tgaaaccgaa gtgtccgccc
     4501 gagaagccgt tttcggaaac cgggtcaaac ccggagaggt catcgtgatt cgctacgaag
     4561 gccccaaagg cggtccgggc atgccggaga tgctcagtgt gaccgccgcc ctggtgggaa
     4621 tggggttggc cgagtccgta tccctggtga ccgacggacg tttttccggt tcaactcgcg
     4681 gcccttgcat cggccatatc gtccccgaag cccaggatgg aggccccatt gccctggtca
     4741 gggatggtga ctccattgct atcgatatcc ccaaccgccg tctggacctg cgcgttcctc
     4801 ccgaagaact ggagagacgg agaaaggcct ggacgccccc cccgctgaaa gtcaaccgag
     4861 gctaccttaa gacctttgcc gaacgggtca gcagcgcgga taaagggtgc atatccatct
     4921 agccattttg caacgtagca aattggcaag gtagccgttt gaagcttccc gaattgccat
     4981 gagccacatt gccagttgct ggttcttgat gatttttgtc attcgaaaaa gaggtgaaac
     5041 gaggtaaaaa aagatatgct cgacagagtg gtcatcagag agatcagcac catcgtcggc
     5101 aaggaaaatg tggtggagga ccgggagacc cgggtttgct actcctacga cgccacaaat
     5161 atcaagcacc ttcccgacct ggtcgttttt gctcggaccc cccagcacat ctctgagatt
     5221 ctcaaactcg ccaatgaaaa ccgttttcct gtcatcccgc ggggtgccgg aaccggcttt
     5281 actggcggaa ccatcccggt ggaaggcgga gttgtcctgg ttctgaccaa aatgaacagg
     5341 atccttaaga tcgaccagga aaaccttctg gccatcgtgg agccgggggt cgtcacctat
     5401 gacttgcagc aggaagtgga aaagatcggc cttttctacc cgcccgatcc ggccagcctg
     5461 aaaagttcca cccttggcgg gaacgtggcc gaatgcgccg gagggccgag ggccgtcaaa
     5521 tacggagtca ctaaggacta cgtgttgggc ctggaggcgg tcttgcccac gggggagatc
     5581 atctccgccg gggttcagac ggtcaagagc gtggtgggct acgacctgac caagctcctg
     5641 gtgggatcag agggaaccct gggagtgatc accaagatca tcctccgtct cctccctctt
     5701 cccaaagcca aacggaccat gttggtcatc tttcccacca tcgagtcggc ggccacctcc
     5761 gtctcccaga tcatcagctc ccggatcatc cccacgacct tggagtttat ggacaattcc
     5821 acgatccgct gcgtggaaga ctacctccac atggggctgc ccgtggatgc cggcgcactt
     5881 ctgatcatcg aagtggacgg ggcccccgag gccctgaatg gggagatcga agaaatccag
     5941 aaaatctgcc agaccaaccg ggccatggaa atcaaagtgg ccaaagacaa gcaggaagcg
     6001 gaagagctgt ggaaggcccg gcgggcagtt tcggcggcgg tggtcaagct gaacccgacc
     6061 aaaatcaacg aggatgtcac cgttccgcgc tcgaaggtgg ccgacattct ccggcgaacg
     6121 gaggcgatct ccaagaagca cggccttatc atcgtcaact tcggccatgc gggtgacgga
     6181 aacatccatg tgaacgtcct gatcgacaag cgcaaacccg gcgagcagga gaaggcgcat
     6241 gccgcggtga aggagatatt ccaggccgcc ctcgacttag ggggtaccct ttccggggaa
     6301 cacggaatcg gcatcaccaa agccccctat ctccccttgg agctggggga catgggcatc
     6361 gaagtgatga agcggatcaa gaaggccttt gacccaaaca acattctgaa ccctgggaaa
     6421 attttccccg atggggcgaa ggcgaccgtt cactaaaaaa caatttcgaa attcgaaatc
     6481 cgaagcacga aacaaattcg agtgaccaaa attcaagatt ttgggttcat caaagtttga
     6541 tgaactcgta aaaagtccaa aagccctttt ctccgtcatt ccggcgaaag ccggaatcca
     6601 gttattccaa gatgttttgg accccggctt tcgccggggt gacgatgcgg aagtcttttt
     6661 gtgagaccat caagtttggg acattcgaac tccgaatttg cctccggatt caatacttgg
     6721 aattattggg actcacgacc atgaaggaga ttaccgaagt tcaggaagaa gcccggcggt
     6781 gcgtgaagtg cgggaactgc ttggcccagt gtcctgttta tgccgaaacg ctggaagagc
     6841 ctttggcggc ccggggcaag atggctttgg tggagtcgtt gaaggaagac gatcccgaat
     6901 acaccaacag gtattacaaa attctctccc aatgccttct ctgcggcacg tgcgcggaga
     6961 actgcccgaa tggggtatgc ggagacgaaa tcatccgcga agcccgcgcc ctgctcgtag
     7021 aaaaaaaggg actgaccctg cccaagaagg ccatctacaa atattttctc gacactgacc
     7081 gcctcatgcc cctgcttctc aaggcaggag cttcgctgca gcacttcttc ttggagaaga
     7141 tccctgaaga gagcggcctc cggctccgtt tccccatgcc tttcatcgat tcaaagaggt
     7201 tcattccgag cctggccccg gaccccttcc tggacatccg gtccggctgg gtccgcgcgg
     7261 aaaaagaaac catgcgggtg ggtctcttcg tgggctgcgt gagcaactac atgtttccac
     7321 gaattgccca gtccactctc gatcttttcg tgcgcaacgg gatttcggtc tacatcccca
     7381 aagagcagaa gtgctgcggt cttccggcct tcgggtcggg ggatgatgag actccccgtt
     7441 ccatcgcccg taggaacatc aaggcctttg ccgaagaaaa cctggatcag gttatatctc
     7501 attgtgcttc ctgcgcggcc atgcttaagc tcgattatcc cctcctcttc ggcgaagacg
     7561 atcccttccg gaggaaggct ctagccttct cggaaaaagt cagcgatgcc actttgttcc
     7621 tgtcgaaggt catggaaccc cccgccaccg accgccgacc gccgaccaaa tcggttcggg
     7681 tcacttatca tgatccgtgc catttgcgcc gcaagctggg aatcttcaag gagccccggg
     7741 ccctcctgca atccaccccg ggactggaat acgtagagat gaaagacgcc aaccggtgct
     7801 gcggtcaggg gggaagcttc aacgtggcca actacgactt atcattgaaa attctggacc
     7861 ggaagacaaa ggccatagcg gagagcgaag cggacgtggt aaccaccact tgcagcggct
     7921 gcatgctcca gctgatggat ggaatccatc aggcggggtt aaaaaaggaa gtccggcact
     7981 tggtagagat ggtcaatcag gcgaccctgc tttaagaagg gcgcccgtat ttttatttct
     8041 cgtccagcga tttcatgtcg atgaacggaa ccgcccctcc cgaaaaccgg ggcagctgcc
     8101 cgttccactt ctcaatggcc ttgatgttgg cctcgatctg gcgcagcttg atcaaataag
     8161 gagtgacatt ttctttttgc aggcgtagcg attcggcctc tgcctttgcc tgagttattt
     8221 tctgttcggc ttcaatcttg attctctcca ggtccctctg cgctttcata gccaactgtt
     8281 cggccacctg cttggcttcg atggccttct gaaactccac atcccacttg aagttcacca
     8341 cggaaaaatc atccacgacg attttatagg ggtgaagggc ctctttgagg tggtccttca
     8401 tctcattacg gatctgctcc cgcttggaga tcagctccag agccgtgtac cgggcggtaa
     8461 tggctttcac gatctcctgg atcttcgggg aaaccagctt gtccttgtag tcgattccca
     8521 cattctggaa gatccagctg gcctcgctgg gaatcacgtg gtagttcacc gccaccttgg
     8581 tgtggtttac ctgctggtct ttggtcaccg cgtcgcagtc cggctgagtc acgaggatcc
     8641 gaacgtcggc tcttaccacc tgctgataga ccggccaccg gaaagccaga ccctcgggaa
     8701 ggacatcttt ctgcaccgcc ccccagttga ggatcacgcc ccgatgccca gcggggatgg
     8761 tcaggaaagg attcgctacg agaaggatga gcaataagac gatgcccccc acgataattt
     8821 tcagctttct gctcttcaga acccctgtca cccgttcatt catggcattc acgtccattt
     8881 tctgtccctc ccatttttgc cggaacataa atccaccccc tggaatagtc cccttcccgg
     8941 attttatctg ttgattttac atgatttagc ggcatttagc gaattttcct tactgcctgc
     9001 atcctgcccc tgcacccttg ctcttgccct ttgcctcgtt cctcgaacct tatgcctccc
     9061 tcctgttttc ccttgacgcc atcccggcat ttttgttaag tttccgagac ataaggaatc
     9121 ctaatcactt aacagaaagg gaaaggagga cgtcatgaga ttgggaagat tcctgattct
     9181 gttttgcctt ggttttctat ttatcctgtt tgccttcagc ccggccctgg ccgggaagga
     9241 ccacttgatc gtggcttttg aggactcggt caaaaccatg gactattacc agaccaccca
     9301 gagaatcggg atcatcacgg gctacatgat gtacgacccc ctcctgaaga gggacccgaa
     9361 agacggatcc cttcatcctc acctggtcac ggaatggaag gccatcaatg acacgacctg
     9421 ggaattcaag ctgcggccgg gggtcaagtt ccacaatgga aaccccctga acgccgaggc
     9481 catccgcttt accattgagg atcgcattct ggacccggcc cagaagtctc ccacggcggc
     9541 gggctggaaa tgggtcaaga aggtggaggt gaaagacgac ctgactttcc ggatcatcac
     9601 cgacggtccc tatcccctga tcctggaacg cttcaacgtc ctctttccct acgatcctaa
     9661 atgggtgaaa gagatggtgg ctaagaatgg ggaggtctat ctggcccgca atactatggg
     9721 gacggggccc ttcaagttcg tcaagttcat tgagggagac cggatcgaac tggtaaggaa
     9781 tgaaaattac tgggaaaagg gatacccaaa atttcccaag atgaccattc gctttattcc
     9841 cgagcagtcc acccgcattg ccgaactgat ttccggcgga attgacgccg cgctgaacat
     9901 cctgtccgat cagattccca ctattgaaaa aagcaaaaac tcggaaacga tcatcgttcc
     9961 cattcttagg gtcagcttct ggcagtttga cagctcgggc cgcgccccga attctcctcc
    10021 agccctcaaa gatgttcggg tccggagggc tatctggcat gccatcgacc gcgaggccat
    10081 catcaaaaac gtcctggggg ggaatgctga cctggtgaac attcccatga gcccgaagca
    10141 gtttgccgcc gacctttcca tcaaaggcta tgagtacaac ccggagaagg ccaaggtcct
    10201 gttgaaagaa tccggctatg aaaaggggtt taatctcaac gtgtggacgt acacggcacc
    10261 ctgcaagatg gccaatgagg cggccatcgg gtatctagaa aatgtgggga ttaaaggcgt
    10321 aatccgggac tacgtgggcc gggtggggga gatggagaag ttgtgcatag cctccaagac
    10381 cgatggagcc ctgaacatga cctggggatc ctacaacatt tttgaccccg atgccattat
    10441 gacctatttt ttcatgagcc cggaggcgcc ttacgattac accaacgacg ccgaactgat
    10501 tggctggctg cgggaggccc gtacaaccct ggacaagcaa aaacggaaag atctttattt
    10561 caaggctcag aagcggatca tcgaccaggc ttactggatg cccttcttca ctcagcggta
    10621 tattgcggga gtgaacaaac acctaggact caccgtggga atcgacgaag tcccccgatt
    10681 ccagtacgcc acgtggaaat agggcctcat cccatgtagg gcctgatccc tgatcaggcc
    10741 gcaataaaag gccttttcaa agggtagggg cggttcgcga accgccccta cacagcaaga
    10801 caccacagat ctgttgaaca gcagccttaa tagccaatcc tttgaaggga aatagagagg
    10861 aaatcgggaa agagaccgcg ggatgaggac tttcatcatc aaacagctcc ttcaagccgt
    10921 cggggtatgc ctggtcatct ccatgatttc cttcttcctc cttttcctga acaccgaccc
    10981 cgccctcctc cttttacccc cggaagccga agtccaggac atcgccaaat ttaagaagca
    11041 gatgggcctg gaccgcccgg tccccgttca atacctcgac tttctacgca aggtagtatt
    11101 gcatggcgat ttcgggaatt ccttcatggc caaggtcccc gcggccaaac tcgtccggga
    11161 gcggcttccc gccaccgtcg agctggccct ggccgccctc atcctggtca acctggtggc
    11221 cctgccggtg gggatcattt cggccatccg gagatattcg cttctggata atgtggcgac
    11281 cttcaccgcc ctgctcggcc aggctatgcc cctttactgg ttcggcatca tgctcatcat
    11341 catcttcggg gtgtggttga ggtggtttcc catctccggc tccgatacct gggctcacct
    11401 ggttctcccg gcgattaccc tggcatcttg gatccttccc attaacatgc gtctggtgcg
    11461 ctccggaatg cttgacgtgc tgaaccaaga ctacatccgg accgcccggg ccaaggggtt
    11521 ggtggagcgc aaagtcctga tcaaacatgc tttcaaaaac gccgccattc ccgtgatcac
    11581 catcaccgga atgcagttgg gtgcccttct gggaggggcg gtggtaaccg aaacggtatt
    11641 tgcctggccg gggctcggac gattggccgt agattcaatc cgaatggggg actatccggt
    11701 cgtccaggcc attgtggtcg tctttgccct gttcgtggtg atcggcaact ttgcggcgga
    11761 cattctggcc gccctcatcg acccacggat tcgactggaa tagaacgatg gccgaaatcc
    11821 cgacctcaaa cccggaaatc gacgaagcag ccccctcgat cctgagcctt gtgctccgga
    11881 agatgtggca tctccggatg ggggtattgg gggccctgtt gattttttgc cttatcttca
    11941 tcgccctctt ctgcccctac gtgtcacccc atgatccatt caaacaggac attaccaaaa
    12001 gactctggaa gccggccttc atggaagggg gcaaggcaga atatctgctg ggaaccgacc
    12061 atctgggccg ggatctcctc acccgtatct tgtatggaac gcggatcagt ctcgtggtag
    12121 ggcttgcggc ggtcatcctg atgctgacgg tgggagtcac cctcggcctc gtggccggat
    12181 actttggagg aaaggcggat gcagtcattt cttttctggt caattgcatg atgggtttcc
    12241 ctttcattct cctggccatg agcctggtgg ccgtcctggg cgcctccctt cagaatatca
    12301 tcatcgccct ggggcttacc ggatggccgg tctttacccg cgtcacccgg atcgaaacga
    12361 tgaaactccg ggatcgggag ttcgtcctgg cggccaagtc cctggctttt tccacccgac
    12421 ggattttggt ccgccatatt ctccccaacc tgcttcccgc catcctggtc ttggcgacag
    12481 tggaagtggc cagggccatt attcgcgagt ccctcctctc cttcctgggc ctgggaattc
    12541 agccccccac cccctcctgg ggggtcatgc tggcggaagg gcgggattac atgcttctgc
    12601 aatggtggct ggccgctttc cccgggatgg ccattttcat gtcggccttg gggatcaatc
    12661 tcctgggcga cgctctgagg gatctggttg acccctacct gaggaaaacc tgatggatcg
    12721 gccagccccc gaatcccggc ttacttcgaa ttcaatctcg gccaccgaat ccggcgatga
    12781 tttcctgctc cgggtccagg gcctcaagaa gtatttcccc atccgcaagg ggctcctggg
    12841 gcggaccgtc gggtatgtct acgccgtgga cggagtcagc ttcgccatcc gccgggggga
    12901 aaccctcggg ctggtaggtg agtcggggtg cggcaaatcg accatcggcc ggtgcctcct
    12961 gcggctatac gaggctaccg acggccagat tttcttccag gggatggaca tcagcaagct
    13021 cagccccggg gagatgattc aattccgcac ccgtattcag atgatcttcc aggaccctta
    13081 ctcttccttg aatccccggc tccgggtgcg ggagatcatc ggcgagtcgc tccgtgtcca
    13141 taacctggtt ccccaggggg actggcagga ccgggtaagg gagctcctgg ccgaagtggg
    13201 cctgcagccg gatcacatgg cccgttaccc ccatgagttt tcgggcgggc agcggcagcg
    13261 gatcggggtg gcccggtccc tggccatgaa tccagacctc atcgtggccg atgaaccggt
    13321 ttcggcgctg gacgtgagca tccaggccca ggtcatcaac cttctggact ccctcaagga
    13381 taaacacggc ctctcttaca tcgtgatcgc ccacgacctg gccgtggtgg agcacatctc
    13441 cgaccggatc gcggtcatgt acctgggcaa gctggtggag acggcccgcg accgggatct
    13501 ctacctgaat cccaaacatc catacaccga ggccctgctt tcggccgtac ccattccggc
    13561 cccggggggg agagagcgga ggaaatctcg gaaattgatc caaggggatg tggcctctcc
    13621 catcaatccg ccgtcggggt gccggtttca cacccgctgc ctgaaaacga tggaggaatg
    13681 ccgccggatc gatcctcccc tgatcgagat cgggccggaa catcacgtgg cctgtcttct
    13741 ttacggaggg ggcaggcctt gagagaatct ctgattcaac tgaagaatct gaccacccac
    13801 ttttttaccc ccgagggggt ggtaaaggcc gtagagcggg ttacttttgg gatcgataag
    13861 ggccggactc tgggtgttct gggcgaatcg ggctgcggca aatcggtcac cgctttgacc
    13921 atcatgcgcc tcattccgga cccccccggg aagattctgg aaggagaaat tacatttgag
    13981 ggttccgacc ttctccagct ttctcccggg gatatgcgcc aggtccgggg cagggacatt
    14041 gccatgatct tccaggagcc catgacctct ctgaacccgg tttacaccat tggggaccaa
    14101 attgcggaaa cgtatgtcat ccaccagcgg atgggcaagc cggaagcctt tcagcgggcg
    14161 gcggaaatgc tccggttggt gggcattccc gctccggaga ggagggttca cgaatatcct
    14221 catcagctct ctggaggaat gcgccagcgg gccatgatcg ccatggccat gtcctgccgc
    14281 cccaagctcc tcatcgccga cgagccgacc accgctctgg acgtgaccat ccaggcccag
    14341 attttggacc ttatgctcgg actgcaggaa gaggtgggca tggccatcat gatcatcacc
    14401 cacgacctgg gggtcattgc cgaggtgagc gacgacgtgg tggtcatgta cgccggacag
    14461 gtggtcgagt gcgcttcgat cgaaaccctt ttcctccagc ccaggcaccc ctacactctg
    14521 ggacttatgt cctccatccc ccagctgggc aggaagttca aagaagggaa agcgcgcctc
    14581 aaggaaatcc ccggcctggt ccccaacctc atccgccttc ccccgggctg tctcttccag
    14641 cctcgctgcc ccatggcgtt ccatacctgc acgcagcaaa ggccccccct cttctccctg
    14701 gattcggggc acggcgttcg ctgctggctg gtggagaaag agtcaaaaaa tccctgacct
    14761 caacgattaa aaaacctgtt gggccccccc cttttcgcct cttccccctt aggccttttc
    14821 gattctcttc ctcaccatct cccagctttt ggtgatgaga aagctctcct cctgattatc
    14881 caaaagcgaa aggaaaagtc cggtgggcat ggctagggtc aggatatggg gctcgacgaa
    14941 aggccgggcc gagacgtcga ataaacccag gatggcctga gggtttttct tttccgcctc
    15001 tttcagagga tgggacaaga tggccccgca gccggagctg aaggggatgc ggacggcatc
    15061 catccgctcc gccgcatagt tggttagggt gaacaacccg gacaccacgt cgggggaggc
    15121 aaagaagatg acgacttctg gctcttcggc gttcgtcagc ttatccagcg gcttgaagag
    15181 gcaatacttg gccggagctt ttcgaggctg cgtggaggca aaatatttcc tcgcgatctc
    15241 cggggtttta atgtaccgct ccccttccat ttccccagga attccgcagg aaagaaaata
    15301 ttcaatgttg ggtctgggcg tcgactggaa tcccatgtaa tatcccccgc cgaagcagcc
    15361 cacatgatcc gcgtcaaaat aaaccgtgtc cccctcgtgg cgggcccgtt tcagaagggc
    15421 aaacatacag aggtggccct tttctttcgg agaaagccct gctccaggtt tgtgattgct
    15481 gtaaaaagcc gccaggggat gctccttcaa tcccaaccgg tcgtggaatt tctggtattt
    15541 ctgggtatcg attttcatgg aagattcctc ctttcaaaaa aggttctcat gccggaacaa
    15601 agggtcctac gggcacccct ccatgggtac ctttttctcg gacggccaca tagggccgcc
    15661 cctacaatca gggtctcccg cctaatatat tttcaatttc ttctctcttc gcagggaccg
    15721 tttgaattgc cccaggattt tctttgtcaa ccgcccattt tgttggattc aattggatat
    15781 attcacggat cagggttaac tcgttttcct tccggatgac atgttcgtaa taattgcgtt
    15841 gccatagcct acccggaaaa gcaggccagc tctcttgagc caccccttta atatattttg
    15901 tagtggttaa cgacttaaaa cgctggacca cttcaggcaa agaaatcgta ggggcaaccc
    15961 tctgtggttg ccccattctt tttttctcat ctgggcaggc acgggggcct gcccctacgc
    16021 ctaaaatgat aatcccgtgg atatggttgg gcattgtcac aaaagcgtct atgtcaacac
    16081 tagaataatg tctagcaatg tcgatccata ccgactcaac catcctgccg gcatcgctta
    16141 atcgcatttc tccaccgaca atttccccca aaaggcattt tcgcccgttg gtacaaacgg
    16201 ttacaaaata ggctcccgcc tgcgcataat cgtagttagg taaacgaagg gaccgacggt
    16261 gggttctgtt tatcaattgt tttactgaac cccgatggcg acccggcggg tcgctcctgc
    16321 aatttccttg cattattgcg gagcatctgc ttaaaaaaga aaaagaccct caggctgaac
    16381 gctgaaagct gacagctgat ttatcaatcc ccgaaaatca ccccgatcga ccgggctgtg
    16441 cggaccattt ctccgtcggg gcggaccctt ctctgccgac ctccgacttt ctccaacggg
    16501 atgtccttga tctgggttcc tcgcaggcaa accatcctcc cgaattttcc ttccatggcc
    16561 aattctatgg cccttgcccc gaaccgggtc gccaggatgc ggtcgaaggc agagggagac
    16621 cctcctcttt gcacgtggcc cagaaccgta acccgggttt ctacccctgt tcttttctcg
    16681 atctcattcc ccaccctgta accaataccg cccaaacgca aggggtcggt gctgtcttcg
    16741 atcactcgct ggacgaccac ctctcccttt tttggcttgg ctccctcagc cacaaccaca
    16801 atgctgaagc gtttccccag acgccgtctt tccttgatct gttcgctgat cttttcatag
    16861 tcgaaaggaa tctccggaat caggatgaca tctccacctc ccgccacccc cgccatcaag
    16921 gcgatccacc cggcatacct tcccatcacc tccaccacca tgacccggtg gtgggattcg
    16981 gccgtggtgt ggaggcggtc gacggcgccc attgccacct gcagggccgt atcgaaaccg
    17041 aaggtcacat ctgtccccca aaggtcattg tcaatggtct tgggaacgcc aatgatcttg
    17101 actccttttt gggaaagctt gttcccgatg gtcaatgacc catcgcctcc caaaacaaag
    17161 aggacatcga ttttattctt tctcaggttt tttaccgact ggttggatac gtccttaaat
    17221 gtcttttggc cattcaaaac tactgggaaa cgaaagggat catcccggtt ggaagttcct
    17281 aaaatcgttc caccccgggg aagaatcccg gaagcatctt tggattccaa gattcttgcc
    17341 ttgttatgga tcaccccgga ataaccatct ataaagccga ccacctccag gccgtaccgg
    17401 taaatggcgg ttttcacgat ggcccggatt acagcattta atcccgggca gtcgcccccc
    17461 gcggtcaata tcccgatcct tttgatcttg gcgttagaac tcatccttcc tcccaattga
    17521 gtattcagta tctaaaatta gtcctatagt cggttagtct tatagtcttg ttgtcttgca
    17581 gtcgaaaggg gcggtcgttc gttgctcgtt gctatatgct atatgtccgt cttcatccgg
    17641 cccccggtat ctaatacctt ttttcgtcat ttcactccaa aatctgaaaa ggagcaacca
    17701 tggtggggag aggcacttcg attaccaccg gtttcttgag tcctaaggcc tctcgcaaaa
    17761 cttttcccag ttgctcgggg gtggctttca ggcccttggc cccaaaagct tcggccagcc
    17821 tggcaaaatc cgggttgttg acttccgtcc ctacaactct gccttgaaaa ttcaccatct
    17881 ggtcgctttt ggttgaaccg aaggcctgat cgttgaagac cagcgtcacc aggttgattc
    17941 catacctcac tgccgtagcc aattcggcac aggcataaag gaatcctccg tcgccaacga
    18001 cgcagacgac ctgtcggtct ggcgcggcca tcttcgcccc caaggccgtg ggaaaagagt
    18061 accccagagt cgcgaaatag gaggaggtga taaaggtccg ggggcgcctg acctcatagg
    18121 ccagattggc ccagtacccg atgttggtca ctccgcacac aaagatggaa tcgtcggcta
    18181 attcctccct cactgccttg aggatcccaa gttgcagagg agccttctcc tgaagccatt
    18241 ggtggtgttt tttccggaat tggtcaagtt ccgcagatgc ccagcgttcc ttaaggactt
    18301 tcctctttcg ggcttcctcc accaatgccc tcaaggcggc cttggcatcg gcgacgatgg
    18361 ccacctcggc cggataattc atcccgatca tggagggatc ggcgtccagc tggatcaatt
    18421 tctggggagg ccgcagagcg gtagcgggcc gtgcctgctg ccaggtaatc cgtgtgccca
    18481 cggttagaac cacatccgcc tcaggcatgg cccagcggac cggcccaaac ccgtaatagg
    18541 ctccccccag cgaaaggggg tggttttccg ggatcactcc tttgccctgg gccgtcgtgg
    18601 ccaccggcgc tcccaaggct tcagccaggt ctttcatttc cggtgagcta tctgacacga
    18661 tggcgccacc ccccgcccaa atgagcggtt tttgtgcacg cgccaaaagg tccgcggccc
    18721 ggcgaaggac ctcctgatcc gggtccttag ggggctggat ttgggggggg ggaaactcca
    18781 cctccccgct ggacctcatg gtgtcccagg ggacctccac ttcggtggga cgcggccggc
    18841 ccgtggccat ctggcgcatg gcttctcgaa tcgcgtccgg tatctcttcg acccgcatga
    18901 cccgcttgca ccatttggta accgggcgga ccatgtccag ttggtcattt acctcgtgga
    18961 gagccccacc gtcccttccc aggtctttcg tctcgacctg cccagccacg agcaggaccg
    19021 gcgaagagca ggcataggct gttcccaatg cggcgagggt attttggacc ccggggccag
    19081 ggactaccaa tccaactcca ggcctcccct gcacccgggc atatccatca gccatgtaga
    19141 gagccgtttg ttcgtggcgg accacaacca ggcgaaggtc cttttccccg taaagggcgt
    19201 catagacgct catgatctgt accccgggaa tggaaaaaag gtactccacc ccttcccttt
    19261 tcagggcttc aacaactgct tctcctccgg tcattctggg catacgatct ctcctgttcc
    19321 taacccatgg tcaaattcaa cgcctcctaa gctccggcct cttcccgctc gggggggctc
    19381 taaatccttc cattttccgg tcaatccttt cccttcccct tgccctt
//
LOCUS       DASPRR010000244        10603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1023482, whole genome shotgun sequence.
ACCESSION   DASPRR010000244 DASPRR010000000
VERSION     DASPRR010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023482"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..749)
                     /locus_tag="VEL68_09530"
     CDS             complement(3..749)
                     /locus_tag="VEL68_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ36246.1"
                     /translation="MKTKKVETALIVGGGSGMGRAAALELARQEIRVFAADLDLKAAE
                     ETAGLVPAVSSRGEAFQVDVSRSESVSSLFRKLQQRAERLDLLVHTAAILGRTVFIED
                     MTDDEWRQMMAINLDGVFYCCREAVRWMKPHNSGRIILFSSVASLTPTPGALHYSAAK
                     GGINMLAKTLAQEVSKYNIRVNVIAPGYIETSMLKGLPEGFSDYILKRTPLRRLGDVQ
                     EIASLVSFLASPEADFFTGQIFSPNGGLVI"
     gene            complement(761..1507)
                     /locus_tag="VEL68_09535"
     CDS             complement(761..1507)
                     /locus_tag="VEL68_09535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ36247.1"
                     /translation="MVDLKDKTVIITGAGSGIGASMALSFSQRGARLTLAARRLERLK
                     EVARKCPGEVFPLAADLTREADRRRIVQQTLERWGNVDVLVNNAGLGLYGDFLSTNEE
                     DWRKVFEVNLFALVFMTRAVLPHMQSRRSGIIVNMASIGGLIAHSDRVTPYVASKHAV
                     IGFSRGLAKDLAGSGIRVLAVCPHLTDTEFFTASPGAEEMAPIVEKFKSFMDSPEDVA
                     RGILEQLDSDRLVVFPTAKPAKAFEKQRDL"
     gene            complement(1648..2907)
                     /locus_tag="VEL68_09540"
     CDS             complement(1648..2907)
                     /locus_tag="VEL68_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXZ36248.1"
                     /translation="MKKKKEPSNGIRSLTRKGLATRAAILDTAHQVFKDTGYYGSSIS
                     EITRRCGISMGNFYQYFKNKEQVFLELSDLILSRFMARSEILTTTQGPFEKRLEEAIR
                     LLYEHTRENFAFHRILGESELTDRVTIAYYEAMARHYRDFFRRETQAGHIRPLDPNMI
                     AYGLIGVCYFHSLDWGEREKRSLREGIRQMADLILHGVNGPTRWKKPSEGHLLSLPDP
                     APLHAAEGEPLTKGESTRQAIFRAAERVIGRHGINRASISEITREAGVALGTFYVHFG
                     SKGDLIEGLVKYINSQMRRELQRRVARTKDRRDAERVGMIAFYEFLRRHREVYRVVPE
                     CEMIDREVSLWYYKKMAQGYIQGLQQGIERGEIRKLPAVFLTRSLMGLIHFIGLKWIV
                     WNTGAQPEVPPHILKDMITLILFGLKP"
     gene            complement(3638..4762)
                     /locus_tag="VEL68_09545"
     CDS             complement(3638..4762)
                     /locus_tag="VEL68_09545"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ36249.1"
                     /translation="MSKVRGWVMLEPGKMEMQKFDLPKIAEDTALLKVEACGICGSDL
                     HAFRGKMKTAPFPLIPGHEFIGTLVEVGTAATEKMAVIGGGTIKAGDKVAIAPSSLAC
                     GRCWFCLHMPHRPSLCTSRSIYGFNSIKNPPSIWGGYAEYIYLHPKTWVFKLPEGISM
                     EKAVQTEPMATGLRAVERAYNPGEAFIGQGYGVGSRAMVLGAGPIGLMVIVALRFSGA
                     KLIIAQDLIESRLKMAKRMGADLLIDGKLPLEQRIKKVQEMTDGVGPDVVIEAAGAPQ
                     AFKESLTFVRRGGKLVEVGHYTDPGPTDINPWVVCNKDVDIHGSWAYPAIIFKDALTV
                     LAKTPFPVEEVVTHKVSLDDLPKGIDLLGKEGVGKVVVTP"
     gene            complement(5015..5719)
                     /locus_tag="VEL68_09550"
     CDS             complement(5015..5719)
                     /locus_tag="VEL68_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36250.1"
                     /translation="MLDIKNLVSGYKKVSVLHGISIQVEGKEILAVVGSNGAGKTTLL
                     KTISGLLPPFSGEILFEQTPIQGLEPYQIVEKGVVQVPEGRQLFNFLTVQQNLQVGSH
                     VAEARQRRADTLKTIFDLFPVLSERRQQLAGTLSGGEQQMLATARALMARPKMLMMDE
                     PSWGLAPMLVHRLFETICQINQQGCTILLVEQHVQQALSIATRGYVLERGNVFMQGGG
                     QELLKDERLKESYLGM"
     gene            complement(5712..6437)
                     /locus_tag="VEL68_09555"
     CDS             complement(5712..6437)
                     /locus_tag="VEL68_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36251.1"
                     /translation="MKFLEVEKLKKTFGGLVAVQNFDMTIEKGEIVGLIGPNGAGKTT
                     VFNMVAGFYQPDAGHVRFDGKDLVGMKPYQVCALGIGRTFQVTKPFVNNTVLENVMVG
                     AFSRTAHAPEARKKALEVLDFFGFQDHKDVLGHELTTADHKRLELARALATQPRLLLL
                     DESMAGLNPAEKMLLLELLRRVRDQGITLLVVEHDMKAVMTLCERIIMMHRGEKLVEG
                     TPQQVANNPQAIAAYLGEEYAAA"
     gene            complement(6434..7528)
                     /locus_tag="VEL68_09560"
     CDS             complement(6434..7528)
                     /locus_tag="VEL68_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36252.1"
                     /translation="MEKKENGKGPSKNIPLGGWAAATVLVLFLIVPFFIHDPYILHIF
                     VLVFLFAYLGTAWSLVSMAGQLSLGHAALVGIGGYVSTLMFMEWGVTPWLGMIVGGIV
                     CGIIGVIIGYPTFRLRGPYFALTTIAFAEILRIYVENTEEGPLGIPLRGGMGLLVPLK
                     GDAPGVFQFSTKEPYYYIALLMMLAAIGLSFFINRWRMGFYLAAIRSDQDAAEALGIN
                     STKYKLMAMAVSCVLAALGGTFYAQYFRYINPERAMGLSMSIEIALVGVVGGWQTVLG
                     PTIGSILLSPSGELVRAYLGGTYAGLHLLLYGLLIMVVVLFMPNGLNDPLVRLFKKWD
                     AELRGTTGGPKDVLPPDRGRTSPLPGRRES"
     gene            complement(7539..8429)
                     /locus_tag="VEL68_09565"
     CDS             complement(7539..8429)
                     /locus_tag="VEL68_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36253.1"
                     /translation="MDFELLASSFLMGLMLGGLYAAVAVGLSLIFGIIRVINFAHGAF
                     LMVAMYASFWLWYFFGIDPYISMIPVAVLFFYFGYYVQKWLIRPIFIREKALVIEPLG
                     VLLLTAGFDLVLSNLALLFFKSDFRAVVSPAASATIDLGFVDLNIPRLIVFILSFVIT
                     GALYWLLNYTEKGVAIRSVGQNREAAALCGVNVYRIYNFTFGIGTAALALAGAAMVPF
                     YYVNPSMGLSLGIKSFIVVVLGGLGSVPGALIGGLILGVVESVTAQFVMSTSSVIFSF
                     LLFILVLFTRPKGIMGVLET"
     gene            complement(8664..9959)
                     /locus_tag="VEL68_09570"
     CDS             complement(8664..9959)
                     /locus_tag="VEL68_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36254.1"
                     /translation="MKKSLFVGWASLIVFGLLFLVPGILKAAEDVKVGEVLPLSGAVA
                     PQGSQLKAGSEIAADEINAAGGIKSMGGAKIKLVFGDSKSTPDGGAAETERLCTLEKV
                     SIILGAFQSGVTFPASEVAERYKTPWMVNVAVKDEITERGFKYVFRDFNKASYDLKEM
                     LAAAELFAEETKKKAKTVALLYEGTDWGRSSAQFAKKFWPEKGYKIILDESYPQNISD
                     FTPQILKIKAAKPDLLIIAMYTPDHILFSKQQMEQKLYVPYGLWSVGAGAEDPSFYKA
                     VPPRSVAYMFVQDDWDTAAPKRAWFPAVDKRSKEKLGYGAAAYVAAGYGTIYLVKDAL
                     ERAASADREKLREAIVATDITEKNCGRIERKAENGQTYCPALVRGIQRIKFDENGQNT
                     YSHGNITQNQKGEKITFWPTEMRPPGAKLAWPIPPWDKR"
BASE COUNT         2387 a         3060 c         2827 g         2329 t
ORIGIN      
        1 ggtcaaatca ccagcccgcc gttcgggctg aaaatctgtc cggtaaagaa atccgcttcg
       61 ggggacgcca ggaaagagac cagactggcg atctcctgta catcgcccag acgtcggagt
      121 ggagtcctct tgagaatgta atcggagaac ccttcgggca aacccttcag catggatgtt
      181 tcgatgtagc caggggcgat cacattcacc cgaatgttgt acttggatac ttcctgggcc
      241 agggtcttgg ccagcatgtt gatgcctccc ttggccgcgc tgtaatgcag ggctccgggc
      301 gtaggagtca gggaagccac cgaactgaaa aggatgatcc gtcctgaatt gtggggtttc
      361 atccagcgaa cggcctcgcg acagcagtaa aagactccgt cgaggttgat cgccatcatc
      421 tgccgccatt catcgtcagt catgtcctcg atgaaaaccg ttcggcccaa aatggccgcg
      481 gtatgaacca ggaggtccag ccgttccgcc cgctgctgca attttcggaa cagagacgaa
      541 accgattcgc tccgggaaac gtcaacctgg aaggcttccc cccgggatga aaccgccggg
      601 accaagccag ctgtctcctc cgccgccttc aggtcgaggt cggcggcaaa gacccgaatt
      661 tcctggcggg ccaattccag ggctgcggct ctccccatcc cggacccccc gccaacgatc
      721 agggcggttt cgactttctt ggtcttcata ctccctctga tcaaagatcc cgttgcttct
      781 caaaagcctt agcaggcttg gccgtgggaa aaaccaccag ccggtccgaa tccagttgct
      841 ccaagattcc ccgggccaca tcctcggggc tgtccataaa gcttttgaat ttttccacaa
      901 tcggcgccat ctcctccgca ccgggagatg ccgtgaaaaa ttccgtgtcc gtcagatggg
      961 ggcagaccgc aagtacccgg atgccgcttc ccgccaggtc cttggccagc ccccgcgaaa
     1021 aaccgatcac cgcatgctta ctggctacgt agggagtcac tctgtcggag tgggcgatga
     1081 gccccccgat ggaggccatg ttgacgatga ttccgctcct ccgggactgc atgtggggca
     1141 ggactgcccg ggtcatgaaa accagggcga agaggttgac ttcgaagacc ttccgccaat
     1201 cctcctcgtt ggttgaaagg aaatccccgt agagccccaa gccggcgttg ttcaccagga
     1261 catccacatt cccccatctc tccagggtct gttggacgat tctccttcga tcggcctccc
     1321 tcgtgaggtc tgcggcaagg gggaaaacct ctcctggaca tttccgggca acctctttca
     1381 gccgctccag gcgacgggct gccagggtga ggcgcgcccc tctctgagag aaggaaagcg
     1441 ccatggaggc cccgattccc gaaccggccc cggttatgat tacggtcttg tccttcaggt
     1501 caaccatcag aaggaccctt tttagagata tgaatcaact gtcactttta tatataaaaa
     1561 aatgggaatt tttgtcaagg gaaaaatatt taaagtttcg ggttctggac ttgcctggga
     1621 tagcgaatct gtccgctagt cgcaactcta tggtttaagg ccaaaaagga tcagcgtaat
     1681 catgtccttt aaaatatggg gaggaacttc cggttgggcg cccgtattcc agacgatcca
     1741 cttgaggccg atgaaatgaa tcaaccccat gagggaccgc gtcaaaaaga ccgccggcag
     1801 tttgcggatt tctccccttt cgatcccctg ctgaagcccc tggatgtatc cctgggccat
     1861 ttttttgtag taccacaggc tgacctcgcg gtcgatcatc tcgcattccg ggaccacccg
     1921 gtagacctcc cggtgtctcc ggaggaattc ataaaaagca atcatgccca cccgttcggc
     1981 gtcccgccga tccttcgttc gggccactct tcgctggagt tcccggcgca tctgagagtt
     2041 gatgtatttg accaggcctt cgatcaaatc ccccttgctg ccgaagtgga cgtaaaaagt
     2101 ccccagggcc actccggctt cacgggtgat ctcggatatg cttgcccggt tgattccgtg
     2161 ccgaccgatt accctttccg ccgcacggaa aatggcctgt cgggtgctct cccccttggt
     2221 caagggttcc ccctccgctg cgtgaagggg ggccgggtcc ggaagggaaa gcagatggcc
     2281 ctcggacggt tttttccagc gggtcggccc gttgacccca tgcaggatca aatcggccat
     2341 ttgccggatc ccctcgcgaa gcgatctctt ctccctctcc ccccaatcca gggaatgaaa
     2401 gtaacagacc ccgataaggc cgtatgcgat catgttgggg tccaaggggc ggatatgtcc
     2461 ggcctgggtc tcccggcgga agaaatcccg gtaatgacgg gccatagcct cgtaataagc
     2521 gatcgtcacc cggtcggtaa gctcggactc gcccagaatc cggtgaaaag cgaagttctc
     2581 acgggtgtgt tcgtacagga gccgaatggc ttcctccagt cgtttttcga atggtccctg
     2641 ggttgtggta aggatttcgg accgggccat gaatcgcgac aaaatgaggt cgctcaattc
     2701 caggaaaacc tgctctttgt tcttgaagta ttgatagaag ttccccatgg aaatcccgca
     2761 gcgccgggtg atctccgaaa tggaggaacc gtaatagccc gtgtctttga agacctggtg
     2821 ggcggtgtcc agaatggccg cccgggtggc aagacccttc cgggtcaagg aacgtatacc
     2881 gtttgatggt tctttcttct tcttcatgag atcatccaaa aaactgaatc atctatcact
     2941 tttgagcatt atattgattt tcttctcccg acgcaactct ttttggcaag ccggaaagct
     3001 tgggtgcggg taatccccca accgggactg agaaaaaggt caaatgatca gtatactcat
     3061 aacggcgtaa accgcctcac ccaggccagg tatccggacg ccactacaaa gactttcgga
     3121 tcgtggagag ggggacgtgg gtgtaaagtg agcttgtttg tactttagaa aaaggaagaa
     3181 aagggaagca ggtcttcgaa cagattcctg ggcacctgaa gaaatgaatc tatagccgag
     3241 gacagtcgga gggaggctgg gtgatggtga accggggggc cttggtggcc gaggtcttgg
     3301 gattcgacca gtgttagccg ggattactcg gaatcagtgg caagaattag tcggaataaa
     3361 catcaccccg tctgccgttt aatcatttcc tcgaaaaatg ggccactaaa cccctggaaa
     3421 gaaatcgccc tccccaattt tctgagaagg gcgaggttat gtctaggatc attatacaca
     3481 gcgtcataaa tcggcgccgt atatcagggc taaagccctg agttacaagc acccctgaac
     3541 ctgtaactca gctctttagg gctgactgca cgacgtggag ggcgatgatg ctgtattact
     3601 tatgccgctg cgtttaaacc ccaggctgac cctgcattta gggcgtgacc actaccttcc
     3661 caactccttc ctttccaagg aggtcgatac ccttgggcag gtcatccagg gaaaccttgt
     3721 gggtcaccac ttcttccacc gggaaagggg tcttggccaa gacggtcaag gcatccttga
     3781 agatgatggc cggataagcc catgacccat gaatgtctac atctttattg cagaccaccc
     3841 agggattgat atcggtgggc ccgggatcgg tatagtgtcc aacctccacg agcttgccgc
     3901 cgcggcgtac gaaggttagg gattctttga aggcttgggg ggcgccggct gcctcgatca
     3961 ccacatccgg gcccacaccg tcggtcattt cctggacttt tttgatcctc tgttccagcg
     4021 gaagctttcc atcgatgaga aggtccgccc ccattcgttt cgccatcttc aagcggcttt
     4081 cgatcaggtc ctgggcgatg atcagcttgg cgccgctgaa acgcagagcg acaatgacca
     4141 tgagtccgat cggcccggct cccaggacca tcgcccggct gcccaccccg taaccttgtc
     4201 cgatgaaagc ttctccgggg ttataggcgc gctcgactgc cctgagaccg gtggccatgg
     4261 gttccgtctg gaccgccttt tccatcgaaa ttccttcggg cagtttgaaa acccacgtct
     4321 tgggatgcag gtagatgtac tcggcatagc cgccccagat gctcggggga ttcttgatcg
     4381 agttaaagcc gtaaatggaa cggctggtgc agagagaggg ccgatggggc atatgcagac
     4441 aaaaccagca gcgaccgcag gccagcgaac tcggagcaat ggctactttg tctcccgcct
     4501 tgatggttcc tccgccaatg acggccattt tttcggttgc cgccgttcct acctcgacca
     4561 gcgtaccgat gaattcgtgg cccggaatta ggggaaaagg cgcagtcttc atcttccctc
     4621 taaaggcgtg gagatcgctc ccgcagatgc cacaggcctc caccttcaaa agggccgtat
     4681 cctcggcgat tttgggtagg tcgaatttct gcatctccat ctttcccggt tccagcataa
     4741 cccagcctct cactttactc atcctgattc tcctttcttc tgttccagtt atcatatatt
     4801 tgatgatctc gtaaagaatc tcccgaagcg tcaccccggc ggaagccggg gtccagacgt
     4861 cgtcccggcg aaagccggga accatctcaa agactggatc ccggttttca ccgggaaccc
     4921 tggattccgg ctttcgccgg aatgacaaat tttgttggat tccgactttt tacttgatca
     4981 tcatacttgg ttagcttacc tttcttcccc ttcattacat ccccaaatag ctttctttca
     5041 gccgttcatc tttcaaaagt tcctggcccc ctccctgcat aaacacattc ccgcgctcca
     5101 gcacataacc ccgggtggca atggagagcg cctgttgaac atgctgttct accagcagaa
     5161 tggtgcagcc ttgttggttg atctggcaga tggtttcaaa gaggcggtgc accagcatgg
     5221 gggccaaccc ccaggagggt tcatccatca tcagcatttt cggcctagcc ataagcgcgc
     5281 gcgccgtggc cagcatctgc tgctccccac cagacagggt gcccgccaac tgctgccgcc
     5341 gctcggacag aaccggaaag aggtcgaaga tggttttcag cgtatccgcc cgccgctggc
     5401 gcgcctccgc aacatgggac cccacctgca gattctgctg cacggtcaaa aaattaaata
     5461 gttggcgccc ctcggggacc tgaaccactc ccttctctac gatctggtag ggctccaggc
     5521 cctggatggg agtttgctcg aagagaatct cgccggaaaa tggcggcagc aacccggaga
     5581 tggtcttcaa gagggtcgtc ttccctgccc cgttggatcc gacgacggcc aggatttctt
     5641 tcccttccac ttgaatcgag atcccgtgca ggacggaaac ttttttatac cccgaaacga
     5701 ggttcttgat gtcaagcagc ggcatattct tctcctaaat aagccgcaat cgcctggggg
     5761 ttattggcca cctgctgggg agtcccttcc accagttttt ccccccggtg catcatgatg
     5821 atccgctcgc aaagggtcat gaccgccttc atgtcatgtt ccaccaccag gagggtgatg
     5881 ccctggtccc gaacgcgccg taaaagttct aagaggagca tcttttccgc cgggttgagc
     5941 ccggccatgc tttcatccaa taaaagcagg cggggctgcg tggccaaggc ccgggccagc
     6001 tccagccgct tgtggtcggc ggtggtcagc tcgtggccca gaacgtcctt atggtcctgg
     6061 aatccaaaaa agtccaggac ctccaatgcc ttcttccggg cttcgggggc atgggccgtc
     6121 cgggaaaagg ctcccaccat cacgttttca aggaccgtat tgttgacaaa gggtttggtc
     6181 acttggaagg tccggccaat gcccagcgca cagacctggt agggcttcat accgaccagg
     6241 tcttttccat cgaaccgcac atgcccggca tccggctggt agaatcccgc caccatgttg
     6301 aagacggtgg tcttgccggc accgttcggg ccgattaggc cgacgatttc tcccttctcg
     6361 atcgtcatat cgaaattctg gaccgccacc aatccgccga aggtcttctt cagtttttca
     6421 acttctagga atttcatgat tctctcctcc cggggagtgg ggatgttcgg ccccgatccg
     6481 ggggcaagac atcctttggg cctccggtcg tccctctcag ttcggcatcc cactttttaa
     6541 aaagcctgac cagcggatcg ttgagcccgt tgggcatgaa gaggaccacc accatgatca
     6601 ggagcccgta aaggaggagg tggaggccgg cataagtgcc cccgagatag gcccggacca
     6661 attcgccgct ggggctcagc aagatggaac cgatggtggg acccaagacc gtctgccatc
     6721 cgcccaccac cccaacgagg gcaatctcaa tcgacatgct caggcccatg gcccgttccg
     6781 gattgatgta ccgaaagtac tgggcataga acgttccacc cagggccgcc aaaacgcagc
     6841 tgaccgccat ggccattaac ttatacttcg tggaattaat tcccagggcc tcggcggcat
     6901 cttgatcgct gcggatggcg gccaggtaaa aacccatgcg ccagcggttg atgaagaagc
     6961 tcagcccaat cgcggccaac atcatgagca aggcgatgta gtaatacggt tccttggtgc
     7021 tgaactggaa aacccccggg gcatcccctt tgaggggcac cagtaagccc atgcctcctc
     7081 tgaggggaat gcccaaagga ccttcctcgg tgttttccac ataaatccga agaatctctg
     7141 caaaagcaat agtggtgagg gcgaaatacg ggccccgcag ccggaacgtg ggatagccga
     7201 tgatcacccc gatgatgccg cagacgatac ccccgacaat catccccagc cagggggtaa
     7261 cgccccattc cataaacatc aaagtcgaaa cgtacccccc gatccccacg agggctgcat
     7321 ggccgagcga gagctggccc gccatgctca ccaggctcca ggccgtcccc aaataggcga
     7381 agaggaacac caggacgaag atgtggagga tgtaaggatc atgaatgaag aacggaacga
     7441 tcagaaatag aaccaaaacc gtcgccgctg cccacccgcc caagggaata tttttcgatg
     7501 gccctttccc gttttccttt ttctccatcg atcccccctt aggtttccaa gacacccatg
     7561 atgcctttgg gccgggtaaa gagcactagg atgaacaata aaaaggagaa aatcacagag
     7621 gaggtcgaca tcacaaattg cgccgttacg gattcgacga ccccgagaat caaccccccg
     7681 atgagggcac cgggtacgct ccccaatccg cccagaacca caacgatgaa ggatttgatc
     7741 cccagagaga gccccatgga tgggttcacg tagtagaacg gaaccatggc agcgccggct
     7801 aaggccaagg ctgccgtccc gatgccgaag gtaaaattat aaatccgata aacgttcacc
     7861 ccgcagaggg ccgccgcctc ccggttctgg cccacggaac gaatggcaac gcccttctcg
     7921 gtgtaattca gaagccagta aagggcgccg gtaatcacaa aggacagaat aaatacgatc
     7981 aggcggggaa tgttcaaatc gacgaagccc agatcaatcg tcgccgatgc cgcgggggaa
     8041 acgacggccc ggaagtcact cttgaagaac aggagcgcca aattgctgag gaccaaatca
     8101 aaacccgccg tcaggaggag gactcctaag ggttcgatca ccagggcctt ttcccggatg
     8161 aagatcggac ggatcaacca cttctggaca taatagccga aatagaaaaa taggacggcc
     8221 accggaatca tcgaaatata ggggtcgatc ccaaagaagt accagagcca gaagctggcg
     8281 tacatggcca ccatgagaaa cgccccgtgg gcaaaattga tgacccggat gatgccgaaa
     8341 atcaggctca gaccgacggc cacggcggcg tagaggcccc ccagcatcaa acccatcaag
     8401 aatgacgatg ccaacaattc aaaatccata gaaaccctcc cctaaagagc tggaattcat
     8461 ggaagacagg tggaaccctg gatcgaaccc ccttggattc aaacccagca tttatatttc
     8521 tctaccggca gaataaacac agacctcacc cttcggaatt ctcggatctt tcccgattgg
     8581 ggtgttcttc caaactgaag ggagaagggc caggaatccc aaactcctga cctctcctcc
     8641 tcgtgatcac ttaggggtgt gatttaccgt ttgtcccagg gtggaatcgg ccaggccagt
     8701 ttggcgcctg ggggccgcat ctccgtgggc caaaaggtga ttttttcgcc tttctggttc
     8761 tgggtaatgt ttccgtggga ataggtgttt tggccgtttt catcaaattt gatgcgctgg
     8821 attccccgga cgagggccgg gcagtaggtc tggccatttt cggccttacg ctcgattcgc
     8881 ccacagttct tttccgtaat gtccgtcgca acgatcgcct cacggagctt ctcccgatcg
     8941 gcggaggcgg cccgttccaa ggcgtccttg acgagataga tggtcccgta cccggccgcc
     9001 acataggcag ccgcgccgta tcctagcttt tccttgctcc ttttgtcgac ggccgggaac
     9061 caggctctct tcggagccgc tgtgtcccaa tcatcctgaa cgaacatgta agccacggac
     9121 cggggaggca ccgctttgta gaaggaagga tcctccgctc ccgcacccac ggaccagagg
     9181 ccatagggga cgtagagctt ctgttccatt tgctgcttgc tgaagagaat atggtccgga
     9241 gtatacatgg cgatgatgag aaggtcgggt tttgcagcct tgattttcaa gatctgggga
     9301 gtgaaatcag aaatgttctg agggtaggat tcgtccagga tgattttata gcctttttcg
     9361 ggccagaatt ttttggcaaa ctgggcggaa gaccggcccc agtccgtccc ttcatagagc
     9421 agggcgacgg tcttggcttt ctttttcgtc tcctcggcga aaagttccgc cgccgccagc
     9481 atctctttca aatcgtagct ggccttgttg aaatcccgga acacatactt gaacccccgt
     9541 tcggtgattt cgtcctttac ggctacattc accatccagg gagttttgta ccgctcggcg
     9601 acttcgctgg ccgggaaggt gactccgctc tggaaagccc ctaggatgat cgagaccttt
     9661 tccaaggtgc acagtctctc cgtctccgcg gccccgccgt cgggagtgct cttgctgtcg
     9721 ccaaaaacca gcttgatctt ggctccgccc atggacttga tgccccccgc ggcattaatt
     9781 tcatcggccg caatttcaga gccggccttc aattgactcc cctggggggc gacggcgccg
     9841 gaaagaggca gaacctcgcc tactttcacg tcttctgccg ccttgaggat tccaggaacc
     9901 aagaatagca gaccaaatac tataagagaa gcccacccga cgaataatga ttttttcata
     9961 ttgacctcct cctttttgtt ggttgattcc tttccgcaaa attccttccc cttccgagag
    10021 ggtcctattt tcccgtgggc gagcctcctt tctacatcct tcttaggatg gaatcttggt
    10081 gtccatgatt cggatttccc gagcgatccg gtatgctgga aggaaataac tttcctatac
    10141 caaaaaggtc aagccctgtc aaatcctatt ccataaaacc gctattctgc cctgcccttt
    10201 tagaggatga aaacaaaagg ggattcgatt actagtcaat ccatcattgt atggatgctg
    10261 aaccctgagc cgttcgtgct gagcttgtcg aagcatgaac ggctcagcgg cgcacccttc
    10321 cacaagcctg tcctgagtga accgttcgtc cttcgacaag ctcaggacga acggtggatc
    10381 gaagggctca gggtgaacgg tggcaggcta agtgaagagc accggggtta gaggaatttt
    10441 cggaaggata ggtcttctga attatggatt ccttagtaaa gtcccaaagc tgtgcgtacg
    10501 gccttcgatg ggggcaggcc gaagccgggc tcatcggggt caataatgag acaagaatct
    10561 acaacggcga aagaacgttc ataaagcggg gcccaccagt cca
//
LOCUS       DASPRR010000245         7220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_864085, whole genome shotgun sequence.
ACCESSION   DASPRR010000245 DASPRR010000000
VERSION     DASPRR010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864085"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            839..3391
                     /gene="glgP"
                     /locus_tag="VEL68_09575"
     CDS             839..3391
                     /gene="glgP"
                     /locus_tag="VEL68_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXZ36255.1"
                     /translation="MKDIVLYTVVPQLPSRLNPLLEMAKNVWFSWNLSVIDLFRSIDQ
                     NLWEETGHNPLAMLGQLSHERVHELFQDEGFLLEMDRIHQEFCRYREDHRKYDFGLET
                     PVDFTAAYFSAEFGLTDCLPIYSGGLGVLAGDFVKSASDLRFPVVGVGLLYQKGYFRQ
                     YLNADGWQQETYPDNDFHNLPVMLEKDEKNSPLTVEVTIQDRQVKVRVWRIQVGRVPL
                     YMLDTNTVENREEDRDITSALYGGDREMRLKQEIVLGIGGIRALRQLGIRPAIYHMNE
                     GHSALANIERIRVLMEENGLSFAEAKEAAYAGSVFTTHTPVPAGIDIFDASLVSSLLG
                     NYLRSIGLSLESFMALGSANENGPRNSFNMAIMALKSSSKTNAVSQLHQTVSRRMWQE
                     IWPFLPEVDIPIDSVTNGIHIPSFISDDLSRLLDRYLGRRWAEDPDNVSVWMRVNKIP
                     DAELWRTHERRRERLIAFTRQRLQEQLQRRGGQKEEIQAASQVLHPEALTIGFARRFA
                     TYKRGNLILKDPARLAKILNNPQHPVQIIFAGKAHPQDNAGKEVIKNIVHLARQPEFR
                     DRLVFIEDYELTVARYMVQGSDIWLNNPRRPLEACGTSGMKAAANGAINMSVLDGWWA
                     EGFRQGLGWAIGSGEEYQEGAYQDFVESQSIYDLLERYAVPLFYDRGKDNLPRGWIGM
                     MKNSMLNLVGQFNSHRMLQDYVHQMYIPSALNWKRMEADNFRGAREFTSWTQHVRQNW
                     PQLRILEKRADTKGLIPFSQALKVEVILQTGQLSPRDLAVDIYFGRVDSKAEFLDREI
                     LSLKDFTQQDGKTVFRGEIPCQRVGRFGFRVRLLPAHPLLSNPYSLGLVLWG"
     gene            3593..4603
                     /locus_tag="VEL68_09580"
     CDS             3593..4603
                     /locus_tag="VEL68_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXZ36256.1"
                     /translation="MGGIFRLSLVWGFILLLAFPPLLLAQDPRLFRQSRILMGTSVEI
                     TVSRVEPKTAEEAMEAAFQEVERINRLMSHYRPDSEVSQISRHAGQKEVRVSPETLEV
                     IERALYFSHLSEGSFDITIGPVFRLWNFREGKIPEEGLLKENLERVDYRKIKIDRARS
                     TVFLEVPRMELDLGAIAKGYAVDRACVVLKKSGIANFLVNAGGDLKVGGEKEKGLSWT
                     IGVRHPRLPSDFIAKLKPKESGVATSGDYEKSFVIDGERYHHILVPSTGLPARECQSV
                     TILAPSAMDADALATTVFVLGPKKGFTLIEKMPSVHAIIVDRRGSVLLSPNWPDGVLL
                     PP"
     gene            4844..6124
                     /locus_tag="VEL68_09585"
     CDS             4844..6124
                     /locus_tag="VEL68_09585"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXZ36257.1"
                     /translation="MDPLPRVFKIHQKIASPRLADVEKEMNAQLDRFGLAGKVKPGER
                     IALTAGSRGIRDKAKVLKVIASRLKGLGAKPFVVPCMGSHGGATAEGQVKMLQHLGIT
                     EEYVGAPIVSSMEVKEVGRTSFGTPVLIDKNICEQADKVVVVNRIKPHTDFDHEVESG
                     ISKMIIIGMGKHKGALMAHRLTIKHGFATMFKEVLPVIRRALPFFFGVGIIENQYDQT
                     ASLHLLEPQNFYEGEKALLKKSKEIMPRLPFKQMDVLVVDEIGKNISGAGMDPNVTGR
                     LFFIGSPPLKEPKITRIFVRDLTSETEGNAIGIGFAEYTTKRLVKKIDPVPTMINSIT
                     GMGPECGRIPISFEQDREALQAAYDNSGVIDPKDLRLVWIKNTLELEYLWASEPMLKE
                     AKANSNIEVVAEPQAIPFDKNGNMVVEWPPRWAD"
     gene            7023..7220
                     /locus_tag="VEL68_09590"
     CDS             7023..7220
                     /locus_tag="VEL68_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36258.1"
                     /translation="MGQESQGQEADKEGQEGKAKQISRITPNKNKSTFPLGNNDGEAK
                     EATVISKSKGKEDDNEGMREP"
BASE COUNT         1752 a         1959 c         1965 g         1544 t
ORIGIN      
        1 ttcttccttt cgatcaagct catgatacca ctgagaatcc ggccagggcc ggttgatcaa
       61 gataaggccg atttgcggat catagcaaga ttttgaacaa gcgaggagat cccagcgcaa
      121 gccacttgat taacggacct cattgagtcc ccgggatgca aatccgtttg ggtcccaaaa
      181 ccaaatcagc ggtttaaaaa ttatattttt gcagtattat cccagtcaag gaaaaacaag
      241 gttgaagccg tcaaggacaa gttgtttctt tgaaatattt caggatcttc ctgttttttc
      301 cgaaaaggaa aatatatccc cgggcaaatg gcagtattct ccctcttcgg ggcagcttcg
      361 cccggattgt cctgtcccct tgcagaatct tgagggtcaa aacattcatc gctcacgagg
      421 aaaggggcgg gaccgcactt tacggacccg gaaagttgaa ccggggcggg ctccggcagc
      481 caatcctctt gacagggtgg cggaaaacac ggtaattttt cagcggcgtc tctgccttga
      541 cctaaacgtt gcatagaatt tcaatggagt cggttcgtca ttcgcgaaag cgggaatcca
      601 ggaagaaatc ctggattccg gggcttcgac gtgagctcag ccgaacgtca agcccggaat
      661 gacggatgca agagctctcc gaaagtgatg tccaggcaca gtatgataat tctgatgaga
      721 cgcaatccaa ataaacggtt ctcattatgc gtcattatga atccatgcgg ctgatgcctt
      781 catttttccg gctggtcggg caatcgccaa attcgcgcta tttcacggga gattgaaaat
      841 gaaagatatt gtcctctaca ccgtcgttcc ccagctccct tcccgcttga acccgcttct
      901 ggaaatggcc aaaaacgtct ggttcagttg gaacctgtcg gtcatcgacc tcttccgctc
      961 catcgaccag aacctgtggg aggaaaccgg gcacaatcct ctggccatgc tgggacagct
     1021 cagccatgag agggtccatg agctctttca agacgaaggt tttctcctgg agatggaccg
     1081 catccaccaa gagttctgcc gttaccggga ggatcaccgg aagtacgatt ttggactgga
     1141 gacacccgtc gacttcactg cggcctattt ctccgccgag ttcgggctga ccgattgcct
     1201 gcccatatac tccggcgggc tgggagtcct ggccggagat tttgtcaagt ccgcgagcga
     1261 tctgcgtttc ccggtggtgg gcgtggggct cctctatcaa aaaggctatt tccgccagta
     1321 tttgaacgcc gacggctggc agcaggaaac ctacccggat aacgatttcc ataaccttcc
     1381 ggtcatgctg gaaaaggacg aaaaaaactc ccccctgacc gtggaagtga ccatccagga
     1441 ccgtcaggtg aaggtccgcg tgtggcgaat ccaggtggga cgcgtgcccc tctacatgct
     1501 ggacacgaac accgtggaaa accgcgagga ggaccgggac atcacctccg ccctttacgg
     1561 cggagaccgg gagatgcgcc tgaagcagga gatcgtgctg gggatcgggg gcattcgggc
     1621 cttgaggcag ctgggcatcc ggccggcgat ctatcacatg aacgaggggc attcggccct
     1681 ggccaacatc gaaagaatcc gggtcctcat ggaggagaat gggctctcct tcgccgaagc
     1741 caaggaagcc gcctatgccg gaagcgtctt caccacccat acccctgtcc cggcggggat
     1801 cgatattttc gatgcttccc tggtaagctc cttgctgggg aattacttgc gctccattgg
     1861 gctttcgctg gagagcttca tggccctcgg atcggccaat gaaaacggcc cccggaattc
     1921 ctttaacatg gccatcatgg ctttgaagag ttcttccaaa accaacgccg tgagccagct
     1981 tcaccaaacc gtctcccgcc gcatgtggca ggaaatttgg ccttttctcc ccgaggtgga
     2041 cattcccatc gacagcgtga ccaacggaat tcacattcct tcctttatct ccgacgacct
     2101 gtcccgcctc ctggatcgtt acctggggag aagatgggcc gaggacccgg acaacgtctc
     2161 ggtgtggatg cgggtcaaca agattcccga tgccgaactc tggcggaccc acgagcggag
     2221 gagggagagg ctcattgctt ttacccgcca gcggctgcag gagcaactgc aacgccgggg
     2281 cggccagaaa gaagagatac aggccgccag ccaggttctt catcccgaag ccctgaccat
     2341 cggctttgcc cggaggttcg ccacctacaa gcggggaaac ctcatcctga aagacccggc
     2401 ccgcctggcc aagatcctga acaatcccca gcacccggtg cagatcatct ttgccggcaa
     2461 ggcccatccg caggacaatg ccgggaaaga ggtgatcaaa aacatcgtcc acctggcgcg
     2521 gcagccggaa ttccgggacc gcctggtctt catcgaagac tatgagttga ccgtggcccg
     2581 gtacatggtc caggggtcgg acatctggct gaacaacccc cgccgccccc tggaagcctg
     2641 cggcaccagc ggcatgaaag ccgcggccaa cggggccatc aacatgagcg tcctcgacgg
     2701 ctggtgggcc gaagggttcc ggcagggcct gggctgggcc atcggctcag gggaggaata
     2761 ccaggaaggc gcctaccagg acttcgtgga aagccagtcc atctacgacc ttctcgagag
     2821 gtacgccgtt cccctgttct atgaccgcgg gaaggacaac cttccccggg gatggatcgg
     2881 gatgatgaaa aattccatgt tgaacctcgt gggccagttc aactctcacc gcatgctcca
     2941 ggattacgtc catcaaatgt atattccctc ggcgctgaat tggaagagga tggaagcgga
     3001 caacttccgg ggggccagag aatttacctc ctggacccaa catgtccggc agaactggcc
     3061 ccagctgcgg attctggaaa agagagccga caccaagggt ctcatccctt tcagccaggc
     3121 gctcaaggtg gaggtgatcc tgcagacggg ccagctctcg ccccgagacc tggcggtgga
     3181 catctatttt ggacgagtcg attccaaggc cgagtttttg gaccgggaaa tcctatccct
     3241 gaaagatttc acccagcagg acggtaagac cgtattccgg ggagagatcc cctgccagag
     3301 agtgggccgc ttcgggttcc gggttcgcct tcttcccgcc catcccctcc tctccaaccc
     3361 ctattccctg gggctggttc tttggggatg acccaagacg tttcaagttt caagttgcaa
     3421 gtttcgggtt tcgggctaga atgatcaagg gccagtttac ctaagggctc aagggcgaca
     3481 ttcgcttaat caaatgggtc ctttaactcg aaactcgaaa ccccgaaggt tcattttttg
     3541 tttccccatt ccttcccact ttcccccctc ccccctgcgg gggagggatg gtgtgggggg
     3601 gattttccgc ctttctcttg tatggggatt cattctcctt ctcgcctttc ctcccctttt
     3661 gctggctcaa gaccccaggc tcttccgcca gtcccgcatc ctcatgggga cttcggtgga
     3721 gatcacggtt tcccgggttg aacccaagac tgcggaagaa gccatggagg cggctttcca
     3781 agaggtcgag cggatcaatc gcctgatgag ccattaccgg ccggacagtg aggtcagcca
     3841 gatcagccgg catgccggcc agaaagaagt ccgggtttct cccgagacgc tagaggtcat
     3901 tgaacgggct ctttatttct cccatttgtc agagggttca ttcgatatca ccattggccc
     3961 cgtctttcgc ctgtggaatt tccgcgaagg aaagatcccc gaggaagggc ttctcaaaga
     4021 gaatttggaa agggtggact atcggaaaat caaaatcgac cgcgctcggt ctacggtatt
     4081 cctggaagtt cctcgaatgg aactcgacct gggggccatt gccaaagggt atgcggtgga
     4141 ccgggcctgc gtagtcctta aaaaaagcgg gatcgcaaac ttcctggtca atgccggcgg
     4201 ggacctcaag gtggggggcg aaaaagaaaa aggactttcc tggacaatcg gggtccggca
     4261 tccccgtctt ccctctgatt ttatcgccaa actcaagccg aaagaatccg gggtggctac
     4321 ctcaggagac tatgaaaaat cttttgtaat agatggtgaa cgataccatc atatcctggt
     4381 cccctccacc ggcctgcccg cccgggaatg tcagagcgta accatcctgg ctccctcggc
     4441 catggacgca gatgctctgg ccaccacggt tttcgtgctc gggccgaaga agggatttac
     4501 cctgatagaa aaaatgccca gtgtccatgc cattatcgtg gaccggaggg ggtccgtcct
     4561 gctctcaccg aactggcccg acggcgtttt attaccgcca tagtctgagg ttccgggttt
     4621 cgcgttaaaa aacgcttctt tgtatttcag tcggaaactt gcaaggacta acattttcaa
     4681 aaaatttggc aatctgcgtt ccgaatacaa cccttccccc atctgcgggg gaggggtaga
     4741 gtgggaaatt attctgcagt ctgcagtccg caatcataga ttccccccca aatatctgat
     4801 ttatgttata atccctttac caattttgaa ggaggaagta tttatggacc ctctaccccg
     4861 cgtgtttaag attcaccaga agatcgcttc ccctaggctg gcggatgtgg agaaggaaat
     4921 gaatgcccag ctcgaccggt tcggtttggc cggcaaagtg aagccgggag agcgaatcgc
     4981 cctcaccgcc ggcagccggg gaatccggga taaagccaag gtgttaaagg tcatcgccag
     5041 ccgtctgaag ggcctcgggg ccaagccctt cgtcgttccc tgcatgggat cgcacggcgg
     5101 agcgacggcc gaggggcaag tgaagatgct ccaacacctg ggcataaccg aggagtatgt
     5161 gggggcgccg attgtctctt ccatggaggt gaaggaggtt gggcggacca gcttcggcac
     5221 tccagtcctc atcgataaga atatctgcga gcaggctgac aaggttgtcg tggtcaaccg
     5281 gatcaagcct cataccgact ttgaccatga agtggaaagc ggcatcagca agatgatcat
     5341 catcgggatg ggtaaacaca aaggggccct gatggcccat cgtctgacca tcaagcatgg
     5401 atttgccacc atgtttaaag aggtattgcc ggtgatccgg cgggctttgc cctttttctt
     5461 cggggtgggc atcatcgaga accagtacga ccagacggct tctcttcacc tgctggagcc
     5521 gcaaaacttc tatgaaggag aaaaagcgct tctgaaaaaa tcaaaggaaa tcatgccccg
     5581 cctccccttt aagcagatgg acgttctggt ggtggacgaa atcgggaaga acatcagcgg
     5641 cgcgggcatg gaccccaatg tgaccgggcg gctcttcttt atcggcagtc cgcccctcaa
     5701 ggagcccaag atcacccgta tctttgtccg ggacctgacc tcggaaacgg aaggaaacgc
     5761 tatcggaatc ggttttgcgg aatacaccac caagcgtttg gtgaagaaga tcgacccggt
     5821 ccccacgatg atcaactcca taaccggtat gggaccggaa tgcggcagga tcccgatttc
     5881 tttcgagcaa gaccgggaag ccctgcaggc ggcttacgat aactcgggag tgattgaccc
     5941 caaagacttg cgcctggtct ggatcaagaa caccctggaa ctggagtatc tctgggcctc
     6001 cgagcccatg ctgaaggagg ccaaggcgaa ctccaacatc gaagtggtgg cggagccgca
     6061 ggccattccc ttcgacaaga acggaaatat ggtcgtggag tggccgccaa ggtgggcgga
     6121 ttaagaacaa ttcccggggg agagtgcaga ggacccaacg cggctatgcc gcacccaaat
     6181 tgcaggggca gtcatgcgaa gagcgtgata tttccccatt ttaggggcag cttcccgcca
     6241 aggtgggagg ctgcccccgc tgcataaaaa gagtccgcac aatatccctt tttgatgtca
     6301 attcctctaa aaaaatccgc ctcaaaataa atcctattcg tcccctgaat ccttccattc
     6361 ataattccca agggcaatct ccttttccct cccagaattt gcagagaaaa acttttgggc
     6421 caaccaggcg cgaaaaacga tcattcccca aatggctaaa taacttcaga ttcccatgcc
     6481 cgctctattc ttgacctaag aattaagcat tgcataaaaa tgctcatgga gtaaggttgt
     6541 cattcccgcg aaagcgggaa tccagaaaga aatcctggat tccgggttaa gcccggaatg
     6601 acggaagcta aagtcctctg aaagtgatat ccggacccag cctgacaatc atcattgcac
     6661 gcgactccat caacggcttt catgctgcgt gaatataaat tagtgtacct gatgggttga
     6721 ttccccgttt aagggattct ttttccgaac aaggatttat cgagcaggtc ggaatgaacc
     6781 ctagttttgc agaaggatta tttaaagaat cgcctgagac aataagagac aataaagtat
     6841 ataaaaagga tgccgataag ggttatggga gggatagtgg aagacaaatt cctcaatatt
     6901 aaagagactt cacagttcct taaactccac accagcacca tcaaccgcct catcgaggcc
     6961 gctgaaatcc caagctacaa agttcggaag cgtcgattgt ttgatcgaga ggagctggtt
     7021 gaatgggtca agagtcacaa ggacaggaag ccgataagga gggccaagaa ggaaaggcga
     7081 agcagattag caggataacc cccaataaaa ataagagtac atttccttta ggcaataatg
     7141 atggagaggc aaaagaagcg acagtcatca gtaaatcaaa gggtaaggag gacgataatg
     7201 aaggcatgcg agaaccatga
//
LOCUS       DASPRR010000246        14033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_61734, whole genome shotgun sequence.
ACCESSION   DASPRR010000246 DASPRR010000000
VERSION     DASPRR010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14033)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14033
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61734"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1440)
                     /locus_tag="VEL68_09595"
     CDS             complement(271..1440)
                     /locus_tag="VEL68_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HXZ36259.1"
                     /translation="MANFFAVSAPGLEPFTAKELKELGLLLIRSSSSPKTFSPGKDEA
                     DLIGGVEFRGSLADLYLANLHLRTASRVLLHLGSFYADTFSDLGRRAKRLSWEDYLRP
                     GRPVALRVTCHKSRLFHSAAVTERVLESIGARLGQTPPTKKLGGEGESNPPQLIVVRL
                     VENRCTFSLDSSGALLHRRGYRLATAKAPLRETLAAGILMASGWDRQSPLIDPFCGAG
                     TIPIEAALMSRKIAPGMRRGFAFMDWPSFDGKTWERLKGSSRQGQPKSGPPILGFDRD
                     AGAIRAAGENAGRADVADSVFFACKAISALDPPPGPGWLVTNPPHGLRLKSHRDLRSL
                     YIRLGRILRAKCPGWHTAILCSSDSLLDLTGIRFERRFPLRSGGLRVTLAKATIG"
     gene            complement(1605..2399)
                     /locus_tag="VEL68_09600"
     CDS             complement(1605..2399)
                     /locus_tag="VEL68_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HXZ36260.1"
                     /translation="MNIFLYLERRTWVHRLDPRGKVLGVLLLFSLCLCFNHPLYMGTI
                     TLSILGIGASARVLFNFWRLRFILILLLIFGTLMWPFFAKGPTPLWSWGPMVVSRESL
                     FYGIAMGLRLSSLVMAGLILLSTTRNEEMTHSLIGLGIPYPVAFAFSTALRLIPTLAG
                     AGATIIQAQVSRGLDLESGNIFRRMGKFIPQAIPLFLYAIRYTNLLAIALESRGFSPG
                     TKRTFYYELKMTPKDYGILVVLGALLAAALYLRLGMGLGAVLPGRL"
     gene            complement(2396..4072)
                     /locus_tag="VEL68_09605"
     CDS             complement(2396..4072)
                     /locus_tag="VEL68_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HXZ36261.1"
                     /translation="MSKAISIEDLTYAYRSQEIPALQHIQNEIQEGSLVVVLGHSGAG
                     KSTLCTSLNGIVPHFFRGRYQGRVFIKDLEVARHKVAEMSRHVGLVLQDFEPQLFSTN
                     VELEMAFGPENLRLPRGEIKRRIETYLPFLGLEHLRRREPASLSGGQKQRLAISSVLT
                     LEPEILVMDEPTTDLDPRGREEVLAIARRLQEEKRTLLIVDSDPETAVRADQVWLMRE
                     GRISAQGPPGEILSDIPGLQACGVKVPGLIELFVEMGWPGKPLDINEAVSLIEKHDLA
                     QKGGLHPPKPSESGMKGNCILRVEGLVYAYPGYGLEALRGINLEIREGEFVALIGQNG
                     SGKTTLAKHFNGLLKPSAGRVLLKGRATTEYKQQQVSRLVGYVFQNPDHQIFCNTVYD
                     EVAFGLKTLGDPLPSMNVRVAEALETVGLMGYEKKVPFALSKGERQRVAVASVLAVRP
                     EILILDEPTTGLDEKQQRALMKMLRSLNARGHTVLIITHSMEVAAEYAARAIVMKDGR
                     VLLDGPTRWAFAQEDRLKEASLHPSPLVRLSNRLGTRALTVKQMVEELKG"
     gene            complement(4092..4838)
                     /locus_tag="VEL68_09610"
     CDS             complement(4092..4838)
                     /locus_tag="VEL68_09610"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HXZ36262.1"
                     /translation="MKAAYTMWKNSRMIILVAVCAAIYGAALIAFKTAIPLIPGITEL
                     RVGQIFPVAFGLLFGPAGAWGTAFGNFIGDLFGGTLGPGSIAGFIGNFLLGYLPYALW
                     THLFPWAHLSREWQGGSWAHRLNYFFVAFIASVACAVVISAFVDLLGIVPYAVLSKII
                     TLNNSLGGWIGLILLVSVFKVTKEQFGLFWAEVMDEEDIGRPLAGFLGAWMVALASVA
                     GLLGGMIPGFPASTVGWVCTLFIFVGCFLL"
     gene            5077..6585
                     /locus_tag="VEL68_09615"
     CDS             5077..6585
                     /locus_tag="VEL68_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36263.1"
                     /translation="MKAKIYSLAIVLMVLLVSSPLLAAPKDTILIAQGTDPTTLDPQD
                     HFEMPAFCVLINIYETLLVRSDDMKIQPLLATSWKIINDTTWEFSLRKGVKFHNGEEF
                     NAAAVKFSLERINDPKNKLKQTTLQGILDRVEVIDDYTIRIITKKPYPYLDAQLCHIG
                     AIMPPKYVQEKGKNHIIGNPVGTGPYKFVRWVKDDHLVLEANENHWKGAPKIKKVIFR
                     PIPEATTRVAGLQTQELDLIVNIPPHLSRVMDWKERSVVAKIPGARIIYMGFDTLHGG
                     PVADKKVRQAIAQGIDVDTIIKKILEGNGVRLGMPLTKYHFGYAPDVKPYLYRPEKAR
                     QLLAEAGYPQGFDLTIHSPSGRYLNDKEVAEAVAGDLQKIGINAKVKIVEWGTYMNMT
                     YNRKAGPSYLLGWGGATMDADGTFFPLLRTDQILSHYSNPSLDDLIEQGRSTMDKAKR
                     LKIYSEACRIVKEEVPFAFMYEQIDLYGVSARLNWAPRPDEKIFVYNMSFKK"
     gene            6776..7237
                     /gene="tsaA"
                     /locus_tag="VEL68_09620"
     CDS             6776..7237
                     /gene="tsaA"
                     /locus_tag="VEL68_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018670806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HXZ36264.1"
                     /translation="MFEGFLLKPIGVVESPVKEPVDENWGTVTSRISLKPEYSPGLLG
                     LGDFSHVIVVTYLHKASFDPARHLQRRPRGLEQMPKVGIFSQRAKDRPNPLGVTAVKL
                     LAVGENHITVRGLDAIDGTPVIDIKPYFPQYDRVDSAVVPAWVEELMKNYF"
     gene            7920..8270
                     /locus_tag="VEL68_09625"
     CDS             7920..8270
                     /locus_tag="VEL68_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXZ36265.1"
                     /translation="MSSIRLPTSQIKKGFLILLGCGVLFILACYPAITSFSPERGKVG
                     TEVTITGKRFGATADENTVRFSGLQAVDVQVPQENTIVARVPEGARTGLISVSTRNGF
                     GESEKDFIVEGKAE"
     gene            8536..8817
                     /locus_tag="VEL68_09630"
     CDS             8536..8817
                     /locus_tag="VEL68_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36266.1"
                     /translation="MKLPWEPSYELIAQEAHALLGLVGVLGTKAFSPNIRTIIWGTVI
                     TLVFGLCKEFVFDHLVEQDSWREDAKDFLFISLGVGLAWAAIGLSYVFK"
     gene            9174..10793
                     /locus_tag="VEL68_09635"
     CDS             9174..10793
                     /locus_tag="VEL68_09635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HXZ36267.1"
                     /translation="MPSTKRGMPVQGSERAALKGARKVGAADPRQRILVTVLVRRRPS
                     ARSLSSEIEEISALQPPERKHLTHEEFAATLGADPGELEKVEEFAHEHGIDVVEVSPA
                     QRRLVLSGTVAAFSKAFGVSLARYKHPKGTFRGRTGPVRVPEDLAPIIEGVFGLDNRP
                     QAKPHMRISGGKRGAVRPRAGGISYTPPQIAKLYDFPAGLNGKGQCIAIIELGGGYRS
                     KDLKTYFGKLGISMPVVKAKSVDGGHNQPTGNPNGPDGEVMLDIEVAGAVAPGAKIVV
                     YFAPNTDAGFLDAVHTAIHDTLNKPSVVSISWGAAESDWTNQAMQAMDKAFQDGAALG
                     VTICCASGDNGSTDGVSDGRLHADFPASSPYALGCGGTRLEGSGSTITKEIVWNEGIS
                     GGATGGGVSDVFSLPKWQANAKVPTSANPGGRIGRGVPDVGGDADPVTGYQVLVDGKQ
                     FTIGGTSAVAPLWAGLIALLNQKLGHSVGYLNPAVYGLPPSSGAFHDISAGNNDITGK
                     HGPYPARSGWDACTGWGSPNGAKLLSALSGP"
     gene            11177..12592
                     /locus_tag="VEL68_09640"
     CDS             11177..12592
                     /locus_tag="VEL68_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HXZ36268.1"
                     /translation="MKNIKFPDIRFLLLFLGGALILSLSVSCGGSGGGSSTSTPNTGS
                     VQVSISDPPTCLVPNGNFQNVWVTITRVRAHTSSTAAPTDSGWVELVDLRNNPVQLDL
                     LSLSSAACLLTVLGTAAGVPSGTYQQIRIVLLSNSPASGESLPAVNNCDGSGLNCVVT
                     AGNATQPLLLSSEAQTGIKIPPGQIAGGSFVVSSGLVTNLNIDFNACSSIVRQGNGQF
                     RLKPVLHAGQVSLSVNSISGRVVDRFTNGAIPNAVVFAEQGDPSTPSLDRVIAQTTTG
                     GDGSFTLCPLSSGTFDVVVTAKTAIQAYSAAVTLQVPVGTIMGNIPLTPQPNVPPATI
                     AGAIQATNVGGALTAADVSLSALQAVNSQLVTIPPFENSTPDVAVDGTTIYALNIPAT
                     NPSVGVFSASPPTFYAPPATGGVLYWANARAFTPMNAALNPGGPDCSPSSFPVIFDST
                     TQLSVTPGSTTTQDFFFTACQ"
     gene            13188..13276
                     /locus_tag="VEL68_09645"
     tRNA            13188..13276
                     /locus_tag="VEL68_09645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13237..13239,aa:Leu,seq:caa)
     gene            13398..13568
                     /locus_tag="VEL68_09650"
     CDS             13398..13568
                     /locus_tag="VEL68_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36269.1"
                     /translation="MESAKEIPTKVEKDRLEEVLEFYRIDLCHEDVSHYDPELIFTDM
                     GKGKNDLSVREK"
BASE COUNT         3245 a         3828 c         3919 g         3041 t
ORIGIN      
        1 aggtcgccca gcaccccgtt gatcttcttc atatcctcgg ggtcgagacg agccaatccc
       61 ttgcccacgt cttcggccag gccctcgatc actttcagtt ccagaatttc catccaggga
      121 ttcttttcga agagagaaaa aattggttta aaggtcagac caattggtaa ttccaatata
      181 ccggatttcc caaagttgtc aagaaaatat ttgcccaaga gcaaggataa aatggcgggc
      241 aaggggtcca gaactcggta ggaaggaata tcatccgatc gtggctttcg ccagagtgac
      301 tcttaaccct ccacttcgca gcgggaatcg ccgctcaaat cggatcccgg tcaggtccag
      361 cagggaatcg ctgctgcaaa gaattgccgt gtgccatcct ggacacttgg cccgcagtat
      421 ccgtcccagc cggatgtaga ggctgcgcag gtcccggtgg cttttcaggc gcaggccatg
      481 agggggattg gtcaccagcc agcccggccc cggaggaggg tccagggccg aaatagcctt
      541 gcaggcgaag aagacactgt ccgccacatc ggcgcgcccg gcattctccc ctgccgcgcg
      601 gatggctccc gcgtcccgat cgaaccccag gatagggggc ccggacttgg gctgcccctg
      661 ccgggatgaa cccttcagtc tttcccaagt cttcccgtcg aagctcggcc agtccatgaa
      721 ggcaaagccc cgtcgcatcc ccggggcgat ttttcgggac atcagcgcgg cttcgatggg
      781 gatcgtaccc gccccgcaaa aagggtcgat caaaggggat tgacggtccc agcccgaagc
      841 catcaaaatc cctgcggcca gagtctccct cagaggggct ttggctgtgg ccagccggta
      901 tccgcgtctg tggaggagtg ctccggatga atccaggctg aaggtgcagc ggttctccac
      961 cagccggacg acgatcaact gcgggggatt gctttcccct tctccaccca atttttttgt
     1021 ggggggggtc tggcccagcc gggctccgat ggactccaga acccgttcgg tcaccgccgc
     1081 agagtgaaag agcctggact tatggcaagt cacgcgcaga gccaccgggc ggccgggcct
     1141 caggtaatct tcccaggaaa gccgtttggc cctccgtccc aggtcggaaa aagtatccgc
     1201 gtagaaagaa ccaagatgga gcaaaacccg gctcgcggtg cgaaggtgca ggttggcaag
     1261 gtaaaggtcg gccagggaac ctcggaattc caccccaccg atcaaatcgg cctcatcctt
     1321 acccggcgaa aaggttttgg gagaagaaga ggagcggatc aggagcaatc ccagctcttt
     1381 cagctcttta gctgtgaaag gctctaatcc cggggcagag acagcaaaaa aattagccat
     1441 ggaatcgctt cttgttgcag gttactggtt gcttttttct tctcctggtt cctcgaattc
     1501 cggccctcgg tctttgctac ctttatcctc cgtgaagcga tcctcaaggc ccaacgggcc
     1561 gttcctcaag tcatgccctt ggcgtgacgc tcctttggcc ttctttacag ccttcccggc
     1621 agcactgctc ccaatcccat ccccagccgc agatagagag cggcggccag aagggctccc
     1681 aggacgacaa gaatcccata atcctttgga gtcatcttga gttcatagta gaaagttcgc
     1741 ttcgtacccg ggctgaatcc cctagattcc agggcgatgg ccaaaagatt cgtgtagcgg
     1801 atggcgtata ggaatagagg gatggcctgg ggaatgaatt ttcccattcg ccggaagatg
     1861 ttccccgact ccaggtccag cccccgagac acctgggcct ggatgatcgt ggcccctgct
     1921 ccggccaggg tcggaatcaa ccggagcgcc gtggaaaagg caaaggccac cggataggga
     1981 atgcccagcc cgatgaggct atgggtcatc tcctcgttgc gggtggtgga aagaagtatc
     2041 aacccggcca tgaccaaaga ggaaagacgc agccccatgg cgatcccgta aaaaagagat
     2101 tcccggctca cgaccattgg gccccagctc cacagggggg tcggtccctt ggcgaagaag
     2161 ggccacatga gggtgccaaa aatcaagagc aggataagga tgaaccgaag gcgccagaag
     2221 ttgaagagaa ctcgggcgga ggcgccaatc cccaggatgg acagagttat ggtgcccatg
     2281 tacaaaggat ggttgaaaca aaggcagagg gaaaaaagga ggagaacccc gagcaccttt
     2341 ccgcgcggat cgagacggtg gacccaagtt ctccgttcca ggtacaggaa aatgttcatc
     2401 ccttgagttc ctccaccatc tgcttcacgg tgagggcccg ggtccccagc cggttgctca
     2461 gccgcaccag gggagaggga tgcagggagg cctctttcaa tcgatcctcc tgggcgaagg
     2521 cccaccgcgt cggcccgtcg agcaacaccc tcccatcctt catcacgatc gcccgggcgg
     2581 catattcggc ggcgacctcc atggaatgag tgatgataag gaccgtatga ccccgggcgt
     2641 tgaggcttcg caacatcttc atgagggccc gctgttgttt ctcgtccaac ccggtcgtcg
     2701 gttcatcgag gatcaagatt tccgggcgga cggccaggac cgaagcaacc gccacccgct
     2761 gccgttctcc cttgctgagg gcaaagggca cttttttttc gtatcccatc aatcccaccg
     2821 tctccagggc ctcggccacg cgtacattca tggaaggaag cggatctccc agcgttttca
     2881 gcccaaaagc cacctcgtcg tacaccgtat tgcagaagat ctgatgatcg gggttctgga
     2941 acacgtagcc caccaacctg gagacctgct gttgtttata ttccgtggtg gcccttcctt
     3001 tcaggagaac ccggcctgcg ctcggtttga gaaggccgtt aaaatgttta gccagggtcg
     3061 ttttccccga accgttctgc ccgatcaggg ctacgaattc cccctcccgg atttccaggt
     3121 tgattccccg cagggcttcg agcccatagc ccgggtaggc ataaacgagg ccctccaccc
     3181 gcaggataca attccctttc attccagact ccgaaggttt gggagggtgg agtccgccct
     3241 tctgggcaag gtcatgcttt tcaatcagag aaacagcttc atttatatcc agcggttttc
     3301 ccggccatcc catctcgaca aacagctcga taaggccggg aactttgacg ccgcatgctt
     3361 gtaggccggg gatatctgaa agaatctctc ccggaggtcc ctgggctgag atccgtcctt
     3421 cccgcatgag ccagacctgg tccgcccgaa ccgccgtctc cgggtcggaa tccacgatga
     3481 ggagggttcg cttctcttcc tgcagacggc gggcgatggc caggacttct tcacgccccc
     3541 gcgggtcgag gtccgtggtg ggctcgtcca ttaccaggat ctccggttcc agggtcaaga
     3601 ccgaactgat ggccaggcgc tgcttctgac ctcccgagag gctggcgggt tcccggcggc
     3661 gaagatgttc cagtcccagg aaaggaaggt aggtctcgat tcggcgtttg atctcccccc
     3721 gcggcagacg gaggttttcc gggccaaagg ccatttccag ctccacgttg gtggagaaga
     3781 gctggggctc aaaatcctgg aggaccagcc ccacatggcg ggacatctcc gccaccttgt
     3841 gccgcgctac ttccaggtcc ttgataaata cacgcccctg ataccggccc cggaagaaat
     3901 gggggacgat tccgttcagg gaagtgcaaa gggtggattt gcctgctccg gagtggccca
     3961 ggacgacgac cagcgatcct tcctggattt cattttgaat gtgctggagc gcggggattt
     4021 cctggctccg gtaagcatag gtaaggtctt cgatggaaat agccttagac atggggagtt
     4081 ttttgagaag gttacaggag gaagcatccg acgaaaatga aaagcgtgca aacccagccc
     4141 acggtgctcg ccgggaaccc tggaatcatt cctcccagaa ggcccgcgac agaggcgagg
     4201 gccaccatcc aggctccgag gaagccggcg aggggcctgc cgatatcctc ttcatccatc
     4261 acctcggccc agaaaaggcc gaactgttcc ttggtgacct tgaaaactga caccagaagg
     4321 atcagaccga tccacccgcc cagcgagtta ttaagggtga tgatcttgga aaggacggca
     4381 tagggcacga tccctagaag gtccacgaaa gcgctgatca ccacagcgca ggccacgcta
     4441 gcgatgaagg ccacaaagaa gtaattcaat cgatgggccc agcttcctcc ctgccactcc
     4501 cgtgagagat gagcccaggg aaaaaggtga gtccataggg cgtagggaag gtagccgagg
     4561 aggaaatttc cgatgaatcc ggcgatcgag cccggcccca gggttccccc gaaaaggtcg
     4621 ccgatgaagt ttccgaaagc ggttccccag gcccctgccg ggccgaagag aagcccgaac
     4681 gcgaccggaa atatttggcc cacccgcagc tcggtgatcc cggggatgag ggggatggcg
     4741 gttttaaagg cgatgagggc ggccccgtaa atcgccgcgc agaccgctac caggatgatc
     4801 atccggctgt tcttccacat ggtataggcg gctttcataa aagctctctt ttccggtctt
     4861 gtccgaattt actggatcag aatggaaaag tcaattcccc aatgaagcca agaattctat
     4921 ttacctaggc aggcctttgt ggataaaata catccgaatt tgtgaatgag gggatattta
     4981 tcagccctaa tatgattgtc tcgcacgtct ccggaagaga aaacagggag gggaatctcc
     5041 gaaggtctct cccaagaggg tataggagag gataacatga aggcgaaaat ttattctttg
     5101 gccatcgtcc tgatggttct tctagtttcc agcccgctcc tggcggctcc gaaggacaca
     5161 atcctgatcg cccaggggac cgaccccacc actctggacc cccaggatca ttttgaaatg
     5221 cccgcgttct gcgtcctgat caatatttac gaaacccttc tcgttcggtc cgacgacatg
     5281 aaaattcagc ccctgctggc cacttcgtgg aagattatca acgacaccac ctgggagttt
     5341 tctctccgga agggagtgaa attccataac ggagaggaat tcaacgccgc tgcggtgaaa
     5401 ttcagcctgg aaagaatcaa cgatcccaag aacaagctca agcagacgac ccttcaggga
     5461 atcctcgacc gggttgaggt catcgatgat tacaccataa ggatcattac caagaagccc
     5521 tatccctacc tggatgctca gctatgccac atcggcgcca tcatgccccc caagtacgtt
     5581 caggaaaagg ggaagaatca cattatcggg aacccggtgg ggaccggccc gtataaattt
     5641 gtccgctggg tgaaggacga ccatctggtc ttggaggcca atgaaaacca ctggaaaggg
     5701 gctcctaaaa tcaagaaagt catcttccgt cccatcccgg aagccaccac ccgggtggca
     5761 ggtcttcaga cccaggagct ggacctcatc gtgaacattc ccccgcacct ctcccgggtc
     5821 atggattgga aagaacgttc ggtggtggcc aaaattccgg gcgcccggat catttacatg
     5881 gggtttgaca ctctccatgg ggggccggtg gccgataaaa aagtacggca ggccatcgcc
     5941 cagggtatcg atgtggatac gatcatcaaa aagattttgg aaggaaacgg agtgcggttg
     6001 ggaatgcctc tgacgaagta tcacttcggt tatgctcccg atgtcaagcc gtatctttac
     6061 cgtccggaga aagccaggca actgctcgct gaggccgggt atccccaggg atttgacctg
     6121 acaatccatt ccccgagcgg ccggtatttg aatgacaagg aagtggccga agccgtggcc
     6181 ggcgacctcc agaaaatcgg catcaatgcc aaagttaaaa tcgtcgagtg gggcacttac
     6241 atgaacatga cgtacaaccg gaaagccggg cccagctatc tcctcgggtg gggcggagct
     6301 accatggatg ccgatgggac ttttttcccc ctgctacgca ccgatcaaat tttatcccat
     6361 tattccaacc ccagcttgga tgatctgatt gaacagggac gatccacgat ggataaagcc
     6421 aaacggctga aaatatattc cgaggcctgc cgcatcgtca aagaggaagt ccccttcgct
     6481 tttatgtatg agcaaatcga cctctacgga gtcagtgcgc gtctgaattg ggccccccgc
     6541 ccggatgaga aaatattcgt ttacaatatg tcgttcaaaa agtaattcat ggagagatga
     6601 ggagtttgga aattcctatt tcgccgacgg gaatgctagg gagaattccc ggggtgaaaa
     6661 gcctcacata ccgtaacgaa accaaaggaa ctgcaatccc gtagtaaatc gcgtacagga
     6721 ttatccccgt gcaaccaatg cgcggcctcc ggatttcggg aaaagggaga gcggcatgtt
     6781 tgaaggattt cttttgaaac ccatcggggt cgtggaatcg ccagtaaagg aaccggtaga
     6841 tgaaaattgg gggaccgtga cctccaggat tagtctgaag cctgaataca gccccggcct
     6901 cctgggtctc ggcgactttt cccatgtgat cgtggtcacc tatctccata aagcctcttt
     6961 cgatcccgct cggcacctgc aacgacggcc ccgcggcctg gaacaaatgc ccaaggtcgg
     7021 catattctcc cagcgggcca aagaccgccc gaatccgctc ggggtcacgg cggtgaaact
     7081 cctcgccgtc ggagaaaacc atatcaccgt gcgcggtctc gatgccattg acggaacccc
     7141 ggtcatcgac atcaagccgt actttcccca gtacgaccgg gtcgattcgg ccgtcgttcc
     7201 tgcctgggtc gaggaactca tgaagaacta cttctgaaga acaagaagag cggaaatgct
     7261 ccttttgctc gtgctgggaa tcgggttgtt ctgtcccgtg caggccgtgg ccccgctgtt
     7321 tttggagtga ggcagcgcgt cgggggccaa ttgtctgtgg agattcaccg ttggcagcct
     7381 gttcggcttt tccaccacct ggcttatgat gctcgtttac aaggtcatga gcccgaacat
     7441 cgcttttgga acctgcggcg gcggggcttt tcgcctttcg ccgctggccc tggcttgagt
     7501 gtccaagtct gaagtttcgt cgctgcaaag ggccggccat gtggccatcg ttgccggcat
     7561 gatcctgttg gccgcgggaa agtccgtgac cctatagcca aaaatttttt caactcgcaa
     7621 agaaacttga attttctctt gtcccgctac tgccgttctg ctagacttta gatgatttta
     7681 atcttcggag ccccagtcaa cttagcacca gcctgacttt ctttcttccg ccctctttga
     7741 agggaggtag gatatgcgca agtggtaaaa ccccatttct gaaaaggaga tggggttttt
     7801 attttataaa ggagaggggg aaggcctggc ccggtggaga gacgaaggac atacccggta
     7861 gaatatgcaa tcatacagcg cttttggagg gcttaaccaa aagcgaaggg gggatgaata
     7921 tgtcttccat tcgtttgccc acctcgcaga ttaaaaaagg atttcttatc cttcttggtt
     7981 gtggtgtgct cttcatcctg gcatgttatc cggccataac cagtttctct ccggaaagag
     8041 gaaaagtcgg cacggaagta accattaccg gtaagagatt cggcgctacg gctgacgaga
     8101 ataccgttcg tttctccggg ttacaggcag tcgatgtgca ggtcccccag gagaatacaa
     8161 tcgttgcccg tgtccccgaa ggtgccagaa ccggcctgat ctcggtctct acccggaatg
     8221 gattcggtga aagtgagaaa gacttcattg ttgaggggaa ggccgaatag actttacata
     8281 tgcggaagcc catcttaacc gcgggatgct tacgccaaac ttaggaatca gcggcaggcg
     8341 gttgaggatt ataagatcgc agccaaatta gggaacaagg ccgcgcaaga tgttttgagg
     8401 tcagagggga ttagttggta atcgctcaga aatgtttaac caacggttta agcggtattt
     8461 tgacccaaag cgccccaata gccaagggga tgatgttgga cggttgaatc agatggccag
     8521 atttgggggt aagtaatgaa attaccatgg gagccaagtt acgaattaat agctcaggaa
     8581 gcccatgctt tgcttggcct tgtgggagtc cttgggacca aagctttttc acctaatatc
     8641 cggacgataa tctggggaac ggttattact ttggttttcg ggttatgtaa ggaatttgtt
     8701 tttgaccacc ttgttgagca ggattcgtgg agggaagatg ccaaagattt tttatttatt
     8761 tcactgggtg ttggtttagc ttgggcggca atagggctaa gttatgtatt caaataaaga
     8821 tcatggtaag atggtcataa tcgggaggat agccttaatt tttgatctga tcgagagtta
     8881 ccatgaaatt aaggaccttt gaaggggaat cagttagagg tgaaatgacc actgattttc
     8941 cagttagtct ggacggcctt tcctgtttaa tgggtgaatg gtgatccgtt cttccccgaa
     9001 gaagcagagt ttttcctaga atcggcgact tgaggagagt tggagatgct tgaagagggt
     9061 ggctatgatc ttccgcgttg tgaagcccac gaaggaaccc gtaaacatct ggaaaacgaa
     9121 tccaggggag atgaatccga cgatctctaa aagaagaaaa ggaggaaaag actatgccat
     9181 cgaccaaaag agggatgccc gttcagggta gtgagcgcgc cgctctcaaa ggagcccgca
     9241 aggtgggggc tgccgacccc aggcagcgta tattggtgac cgtgctggtt cggcgccgcc
     9301 catctgcccg aagcctctca tcggagatcg aggagattag tgccctccag ccgcctgagc
     9361 gcaagcacct tacccatgaa gagtttgccg ccacccttgg tgctgacccc ggcgaattgg
     9421 aaaaagtgga ggagtttgct catgagcacg gcatcgacgt agtagaggtc agccccgcgc
     9481 agcggcgctt ggtgttatcg gggaccgtgg ccgccttcag caaggctttt ggagtatctt
     9541 tggcacgcta taagcatccc aagggaactt tccgcgggcg taccgggccc gtgcgcgtgc
     9601 cggaagacct cgccccgatc atagaagggg tcttcggtct cgataatcgg ccgcaggcca
     9661 agcctcacat gcgaatctcc ggtggaaaga ggggagcggt gcggcctcgt gccgggggga
     9721 tctcctacac gccccctcaa atagcaaaac tctatgattt cccggcggga ctcaacggga
     9781 aaggtcagtg tattgcgatc atcgagttgg gcggcggtta ccgcagcaag gacctcaaga
     9841 cctactttgg caagcttgga atttccatgc cggtggtcaa ggccaagtct gttgatgggg
     9901 ggcacaacca gccgacgggt aaccccaatg gcccggatgg agaagtgatg cttgacattg
     9961 aggtagcggg tgctgtggcc cctggggcca agatcgtggt ctactttgcc cccaacaccg
    10021 acgccggttt cctggatgcc gttcacacgg ctattcacga taccctaaat aagccatccg
    10081 tggtctcgat cagctggggt gctgcggaat cagactggac gaatcaggct atgcaggcta
    10141 tggacaaagc ctttcaggat ggcgctgccc tcggcgtgac aatctgctgc gcctccggcg
    10201 acaacggctc aaccgatggg gtgagcgatg gtcgtctcca tgcggacttt ccggcgtcca
    10261 gtccatacgc tctgggctgc gggggcacgc gtttggaagg gtccggcagt acgattacga
    10321 aagagattgt ttggaacgaa gggatcagtg gcggcgcaac cggcgggggg gtaagtgacg
    10381 tcttttctct tccgaaatgg caagcgaatg ccaaggtccc gacatcggca aaccccgggg
    10441 ggcgcatcgg gcggggggtg cccgacgtgg gtggcgatgc cgacccggtc accggctacc
    10501 aagttttggt tgatgggaaa cagtttacca tcggcggaac cagcgcggtg gctcctctct
    10561 gggctggctt gatcgccctc ctcaaccaga aactgggcca ttccgtgggt tatcttaacc
    10621 ctgcggttta cggtttgccc ccttcttccg gcgcatttca tgacatcagc gccggaaata
    10681 acgacatcac ggggaaacac ggcccctatc cggcccgttc gggctgggat gcctgtaccg
    10741 ggtgggggag ccctaatgga gcaaagcttc taagtgcact gagcgggccg taagaaatct
    10801 ttgacatcaa atggctgctt tgctggaggc tgaatacctt ctatccggac acggggaagt
    10861 catcagggga cggcagaaca tcctgcgcaa ctttgcctat acttgcagca accaccacga
    10921 ttctttataa agaacttccc ggttctcctc tgtgcgggga actttaagag aaaggggccg
    10981 gttggtcttc gggcgaatgg tcagcggacc gaaattccat ttgtggcttt aaaatacacg
    11041 attctccatc tcaatatggg taattcttcc catatgatca gtgagaatcc catcctattc
    11101 taggcttggg cagtgtgatt cttcgttccg gtgggagact gtttttagtt ttatccgccg
    11161 gaaaatggag aaggtcatga agaatatcaa attcccggac atccgtttcc ttctcttgtt
    11221 tttaggagga gccctcattc tgtctctttc ggttagctgc gggggtagcg gaggagggag
    11281 ttcgacttca acccccaata cgggttcggt tcaggtaagc atttccgacc ccccgacgtg
    11341 tttggttccc aacgggaact tccagaatgt ctgggtgacg atcacccggg tccgggcgca
    11401 cacttccagt accgcagcgc cgaccgacag cggctgggtg gagctggttg accttcgcaa
    11461 caatcccgtc caactagacc ttttaagtct ctcctccgcc gcttgccttc tgacagttct
    11521 cggcaccgcg gcgggggtcc cctcggggac ttatcaacag attcgtatcg tcctactgag
    11581 caattcacct gcctctggag agtctcttcc ggcggtcaac aactgcgacg ggagcgggtt
    11641 gaactgcgtg gtcacggcgg gcaacgccac gcagccattg ctcctcagca gcgaggctca
    11701 gacgggaatc aagatccccc cgggccagat tgccgggggg agtttcgtcg tttcctccgg
    11761 cctggttacc aacctcaata tcgactttaa tgcctgttct tccatcgtcc ggcaggggaa
    11821 cggccaattc cgcctgaagc cggtcctgca tgcggggcag gtatccctca gcgtcaattc
    11881 cattagcggg agggtcgtgg acagatttac caacggggcg atccccaatg ccgtcgtctt
    11941 cgccgagcaa ggcgacccga gtactcccag cctcgaccgg gtcatcgccc agaccaccac
    12001 cggaggggac gggtcgttta ccctctgccc cctctcctcc gggacgtttg acgtggtggt
    12061 gacggcaaag acggccatcc aggcttacag cgctgcggtt accctccagg ttcccgtggg
    12121 aaccatcatg ggaaatatcc cccttactcc tcagcccaat gtgccgcccg ccaccatcgc
    12181 gggcgcgatc caggccacca atgtgggcgg ggcgctgacc gcggcggatg tgagcctctc
    12241 ggctttacag gcggtcaact cgcagctggt gaccattccc cccttcgaga attcaactcc
    12301 cgatgtggcc gttgacggga caaccattta tgcgctgaat ataccggcca ccaatccctc
    12361 cgtgggagtg ttcagcgcct ctccgccgac cttttacgcc cctccggcca cggggggggt
    12421 cctttactgg gcaaacgctc gtgcctttac tccaatgaat gccgccttga accccggtgg
    12481 ccccgactgc tcgccctcga gttttcccgt cattttcgac tctacgactc agctgtcggt
    12541 aacccccgga agcacaacca cccaggattt cttctttacc gcctgccaat agcccatcct
    12601 tctcccccct tccaatcctt cgcctctccg ggaggcattc ccctaaggcc ggggaagaaa
    12661 tcacgggggc cttatcccct ccgagaggag gacggatttt ccctttgagt agcatgggtg
    12721 gatcaggtcg gcgcgtttgc ccccaaatct gacaactctt tgctgaagtc ttcgcttttg
    12781 atctctatgc cgtggtactt catttcaagt gggaataacc aggctagccc gccctttcat
    12841 aaaacaactg cttggcaggg tgcttttcca ttaccacctt gccgctcaat aatgagggta
    12901 taccatgaaa tattaaatcc atctgactgt cagaaaaaga aggagtagtg gcctaacgga
    12961 aatatccata tggaacctac actatggaaa gctcacaacg gcctttaaaa accatagcaa
    13021 gttcaaatac ttaagtctgg aacaaatctt gctacctgct ttttaaaagc agggtttttg
    13081 gaatacagca tttgggacgg taaaacgcct cttaaaaaat cagagaatta ggtgccaccc
    13141 taaaagtcat tctttaggaa gtggacctaa tcagctagcc aaaacaagga gggatgaccg
    13201 agtggtctgt ggccaagttc tttcaggcct tttcctcaaa aggggaacgt gggttcgaat
    13261 cctactccct ccgccaaaaa tttgggaccg caaattcttg cgatctccat tggtggctcc
    13321 gaacggaaag aggcggactt cctgacatga acggacttaa accaaaagat ctctgggata
    13381 aaagtttaag aaactcgatg gaatcggcaa aggaaatacc aaccaaggtg gagaaagacc
    13441 gcttggaaga ggtccttgaa ttctaccgca ttgacctttg ccacgaagat gtaagtcact
    13501 acgacccgga gttgatcttc acggatatgg ggaaaggcaa aaatgaccta tccgtgcgtg
    13561 agaaataaac gtttaattca tcccccaata tagccttaaa tgaagcgacg aaaggaaaag
    13621 gaaatagcgt aaagggattt gtgaaaagat tgggtagttt aaactagtgt cgcgtccgct
    13681 aagttcagtg aataaattta ccaggagggg gggtggagaa acgatgcgcg ccgccaaaat
    13741 cggccgggaa atggcctgtc tccaggcggt tgcctggtat ccgcaaagcg acgctgccag
    13801 cctccgggaa ttaggctaag agcgcaaaaa tagacgactt ccgaaccctt tagtaagaat
    13861 caactttttc aaagaggaac gaacttctta ttttgacaag cgcagcggtt cttcgccccg
    13921 ctccctgctg gggggcagaa ttatgcacct tatttctgag acgcgacact agatcaaagg
    13981 ggggaaaatg caaataattt tacaagcaat ggtaatagga gctttgcttt tga
//
LOCUS       DASPRR010000247         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1833741, whole genome shotgun sequence.
ACCESSION   DASPRR010000247 DASPRR010000000
VERSION     DASPRR010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833741"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1721
                     /locus_tag="VEL68_09655"
     CDS             237..1721
                     /locus_tag="VEL68_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ36270.1"
                     /translation="MAKQKLSGDKAWYQPLFHPRSVALVGASRDPKKWGFNLLFNIVK
                     GDYRGRIYPINPKEPEILGFKAYPSIAQVPEPVDQAILVVPSPDMPKVIQECGRNGTQ
                     VGVVITAGFGEVDRMGKKVEGEMVALALSLGLRLVGPNCQGIVSTWDASFYAHMPPQF
                     PPPGPLGVVSQSGNLATSLIEAGRQLGVGFSRIISSGNEADLQTPDFLEFLAEDSKTE
                     VILSYLEGVKDGRKFLQIARKVSARKPFLMVKAGQTGAGVKAAFSHTGALAGSPGLFS
                     GLCRQMGIIEAETIEEMVDMAGAFATQPLPSGRRVGVVTLGGGWGVLAADFCSQAGLI
                     VETLTPRMTKELDGVLPPWWNRMNPVDMVAGYRKGDLLTSIELFLNSKRFDGVLLLGL
                     GWRAVRGGFLKSSAVSDDDGMDAAGQDWIDEEEKIFADLQELGRRYKKPILLASDVIQ
                     YTPGFEEAVRRRRVAAYPSLPRAVRAYLGLVERYEFLKRIQRKG"
     gene            2044..2772
                     /locus_tag="VEL68_09660"
     CDS             2044..2772
                     /locus_tag="VEL68_09660"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXZ36271.1"
                     /translation="MEKAKITWLGHASVLVEFGGKAIYFDPWLDDNPACSMKKKDVKK
                     ATVVCATHGHIDHLGDSFEIVRKTGAKLICTPELGFYADSKGLKEGKEVYGLNTGGSW
                     KSDGFRITMVPATHTSEIMGEGWVSGPIQPGSGAVGFVLDIEGGPTVYFSGDTGVCAD
                     MTLIRDLYRPKVAILTAGGKYNMGYREAACAASLIWPEYLIPTHYGTFEDQKLDLDQL
                     EKEMKVRAPGVKLVRIKPGESFMW"
     gene            2879..3169
                     /locus_tag="VEL68_09665"
     CDS             2879..3169
                     /locus_tag="VEL68_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXZ36272.1"
                     /translation="MIELHIFLEPFAGKEKDLESIYWKEYLPGIKIQKGFQRTALLKK
                     RDALREYQIHIAFDSEDLRLQWVASKEHTAIWPKVAALCQRVSWTGFDTVQK"
     gene            3282..>4009
                     /locus_tag="VEL68_09670"
     CDS             3282..>4009
                     /locus_tag="VEL68_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36273.1"
                     /translation="MKRFLQIASICSLILVFAFSAYGAGEPIKVGFPMILSGGGALFG
                     QPSSVGAEMFVKELNAKGGVLGRPIELIVRDCGNTPEEATRVAREIILKEKVDFMVGG
                     LTASQGLALSEVAKTEKVVYIAPISKTTAMTDPGKLHPYVFRAAANTNTEGRSAAVFM
                     AKNPWTKIATLGPDYEFGQMVTKAFVEWMKKIKPEAQIVHQGWPKLGETDYTPFIAPL
                     LAAKPDAAFLSLWGGHFVTFAKQA"
BASE COUNT          950 a         1032 c         1169 g          858 t
ORIGIN      
        1 aaaggagtcc acagcgcggc gtatccgcaa ccaaagaaaa attgaaaatt accacagccc
       61 cccgatcggc tgagaataat tcttcaattg tagggggaac accctgtggc tgcccatcct
      121 gcgggcggtc acagggccgc ccctacttta tttcttgaca gaaatttcag attttgaaca
      181 ttagtattat agggatataa tccagtcgca tcggagaggc gaaaggaatc ttttttatgg
      241 ccaaacaaaa actttcaggg gacaaagcct ggtatcagcc tctttttcat cctcgatcgg
      301 ttgccctcgt gggggcatcc agagacccaa agaaatgggg attcaatctt ttgttcaaca
      361 ttgtgaaggg agactatcgg ggccgtattt atccgatcaa cccaaaagag cctgaaattc
      421 tggggttcaa agcctatcca tcgatcgctc aggttcccga gcctgtggac caggccatcc
      481 tggttgtgcc ttctcccgac atgccgaagg tgatccagga gtgcgggcgg aacggcaccc
      541 aagtgggagt ggttatcacc gcgggatttg gagaggttga ccggatggga aaaaaagtcg
      601 aaggggagat ggtcgctctg gcccttagcc tgggcctgcg tctggtgggc cccaactgcc
      661 aggggatcgt gagcacctgg gacgcttctt tttacgccca catgccacct cagtttcctc
      721 ccccgggccc tctgggggtg gtatcccaga gcggaaacct ggccacctcc ctgattgagg
      781 cgggccgtca gttaggggtc ggcttcagcc ggatcatcag cagcgggaat gaggccgacc
      841 tccagacccc ggattttctg gaattcttgg ccgaagactc gaagaccgaa gtcatcctgt
      901 catacctcga gggggtgaag gacggaagga aatttcttca aatcgcccgg aaggtttcgg
      961 caagaaaacc tttcctgatg gtgaaggcgg gacagaccgg ggcaggggtc aaggccgctt
     1021 tttcccacac cggggctctc gccgggtccc ccggcctttt cagcggtctt tgccggcaga
     1081 tggggatcat tgaggctgag accatcgaag agatggtgga catggcgggg gctttcgcca
     1141 cacagccgct tcccagtgga aggagggtgg gagtcgtgac cctgggcggg ggatggggag
     1201 tgctggccgc ggatttctgc tcccaagccg gcctgatcgt tgaaaccttg accccgcgga
     1261 tgaccaagga gctggacgga gtcctgcccc cctggtggaa tcgaatgaat cccgtggaca
     1321 tggtcgccgg ataccggaag ggagaccttt tgacctccat tgaattgttc ttgaattcaa
     1381 agcgattcga tggagtactt ttactcgggt tggggtggcg ggctgttcgg ggaggattcc
     1441 tgaaatcttc ggcggtttcc gatgacgacg gaatggatgc ggcagggcag gactggattg
     1501 atgaggagga aaagattttt gcggaccttc aggaactggg ccgcaggtat aagaaaccga
     1561 ttcttttggc ctcggacgtg attcaatata caccgggatt tgaggaagcc gttcgccggc
     1621 ggagggtggc tgcttatccc tccctcccgc gggcagtcag ggcctatttg ggcttggtgg
     1681 agagatatga gttcttgaag aggattcaga ggaaagggtg attttcgaat cttgctcttg
     1741 ccaaacgcgg gaccacaacc cgaaacccgg aacccgcgac tctatctaga aagtctttca
     1801 tcctgaatgg ctatgcatta aaatactatt ttgttgacaa accctctcct tttttataaa
     1861 agataaacca agcttagaat tcaagacaag gtgctcccct caagctgcaa ctcagaaaat
     1921 ggcggagaat tcaaatttcg gggcctcatt acccaaacaa tttcattgac gaaaaacttc
     1981 aatgaggcgg gatgaagcac cctgcctttt cacttccaat cccttgccaa aaaggagaag
     2041 attatggaaa aggccaaaat cacctggttg gggcacgctt ccgtgctggt tgaatttggc
     2101 ggaaaggcga tctattttga cccctggctg gacgacaacc cggcatgctc catgaaaaaa
     2161 aaggatgtga agaaggcgac cgtggtatgc gccacccatg gccatattga tcatctcggg
     2221 gattcattcg aaatcgtccg gaagacgggg gccaaactca tatgcacacc cgaactgggc
     2281 ttttatgccg attccaaggg cctcaaggag gggaaggagg tgtacggcct taatacgggc
     2341 ggaagctgga agagcgacgg gttccgtatc accatggttc ctgccaccca tacttcggag
     2401 atcatgggcg agggctgggt ttcaggcccc atccagcccg gctccggggc cgttggcttc
     2461 gtcctggaca tcgagggcgg gccgacggtt tacttttccg gcgacaccgg cgtttgcgcc
     2521 gacatgaccc tcataaggga cctctaccgt ccgaaggtgg cgatcctgac cgcgggtgga
     2581 aagtacaaca tgggctaccg ggaagctgct tgcgccgcct ctctgatctg gcctgagtat
     2641 ctgattccca cccattatgg aacttttgag gatcagaagc ttgaccttga ccagttggag
     2701 aaagaaatga aagtccgggc cccgggggtc aaactggtgc gaatcaagcc cggagagagt
     2761 ttcatgtggt gaggggtcgc cgggaaagaa atgggacgct agggggacga aatgaaaggg
     2821 ttctatttgg gactccgtgc ccgggtgaat tatccatccg cagaaagaag gtgataaaat
     2881 gatcgaactg cacatcttcc tggagccttt tgcggggaaa gaaaaagacc tggaatccat
     2941 ctactggaaa gagtaccttc ccggcataaa aattcagaag ggctttcaaa gaacggccct
     3001 cctgaaaaaa cgcgatgccc tgcgggagta ccagatccat atcgcattcg acagcgaaga
     3061 tttgcggctc cagtgggtcg cctccaagga gcacacagcg atctggccta aagtggctgc
     3121 cctctgccag cgggtatcct ggaccggctt cgacaccgtt caaaaatagc cgggcatcgg
     3181 gtccgccgga aagagatcga accgcgatgc gggacgggca tttttttcca tcaggggaat
     3241 gcccatttaa aaaaaataaa aaaggagagg aggaaatgat catgaaacgc ttcctgcaaa
     3301 tcgcatccat atgtagcttg atcctggttt ttgccttttc ggcctatggg gccggggaac
     3361 ccatcaaagt tggtttccca atgattctat cgggtggcgg agcccttttc ggtcagcctt
     3421 cctccgtggg agcggaaatg ttcgtgaaag agctcaacgc gaagggggga gtcctgggaa
     3481 ggccgatcga attgatcgtt cgcgactgcg ggaacactcc cgaggaagcg actcgagtcg
     3541 cccgggagat tatcctgaaa gaaaaggtgg acttcatggt gggcggcctg accgcatccc
     3601 agggcctggc cctctcggag gttgccaaga cggagaaggt cgtctacatc gcgcccattt
     3661 ccaagaccac ggccatgacc gatcccggca agctgcaccc ctatgttttc cgggctgccg
     3721 ccaatacaaa caccgaaggc cgaagcgccg cggtgttcat ggccaagaat ccttggacca
     3781 agatcgccac tctcggcccg gattatgaat tcggccagat ggtcaccaag gccttcgtgg
     3841 agtggatgaa aaagatcaag cctgaagccc agatcgtcca tcagggatgg ccgaaactgg
     3901 gggagacgga ctatacccct ttcatcgccc cccttctggc cgccaaacca gatgccgctt
     3961 tcctttccct ctggggcggc cattttgtaa cttttgctaa gcaagcgaa
//
LOCUS       DASPRR010000248         5302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_62035, whole genome shotgun sequence.
ACCESSION   DASPRR010000248 DASPRR010000000
VERSION     DASPRR010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62035"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="VEL68_09675"
     CDS             complement(<1..2038)
                     /locus_tag="VEL68_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HXZ36274.1"
                     /translation="MDERHVAKIAAELGLAAGQVQATAGLIAEGGTIPFIARYRKEIT
                     GSLDEVQITAIRDRLEQLVELDKRREAILKSLQERDLLSEELQEKIQAAETLSVLEDI
                     YLPFRPKRRTRATVARERGLEPLAQILFAQEERDPLSEAVPFIDPEKSVSSAEDALAG
                     ARDIIAEWVSEDQAARAALRDLFASRGVFRSKVIAGKEAEGSKYRDYFAWEEPVSQAP
                     SHRILAMRRGEKEGFLNLTISPPEPEALAILERLFVKGENACSQQVRLAVHDSFARLL
                     SPSMETETRLTTKNRADEEAIRVFADNLRQLLMASPLGQKRVLAIDPGLRTGCKVVCL
                     DRQGKLLHTETIFPLLSEKGKEESARRVLELLAEYQVEAVAVGNGTGGRETEAFIRGL
                     PPPSGIPVVLVNESGASIYSASRAARDEFPDHDVTVRGAVSIGRRLMDPLAELVKIDP
                     KSIGVGQYQHDVDQPALKRSLDDVVMSCVNAVGVEVNTTSAQLLTYVSGLGPQLAANI
                     LAYRNEKGPFSSRDELKKVPRLGPKAFEQAAGFLRIRGGKNPLDASAVHPESYAVAQA
                     MAQDLGCTVEDLMREATLRGKIDLSRYVTEKTGLPTLNDILKELAKPGRDPREKFEDF
                     RFAEGIEKIEDLQPGMKLDGIVTNVTAFGAFVDIGVHQDGLVHISQLSDRFVKDP"
     gene            2269..3447
                     /locus_tag="VEL68_09680"
     CDS             2269..3447
                     /locus_tag="VEL68_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HXZ36275.1"
                     /translation="MTRSPSPKSRIVITCAKGVPPFLKEEISSLGLPVRSESIAGVET
                     EGTMEDAMRLNLFLRTGQRVLLFLRQLEAKNADELYAGISRMEWENYVSPEEYLCVTS
                     AVDNPTIRDFRFANLKCKDAIVDRIKKKVGRRPDSGSKRTGVVVDLYWKGNLCLVYLD
                     TSGEPLYRRGYRKIPLHAPMQETLAAAVIQATGWKGRGNFVNPMCGSGTLAVEAALAA
                     LDIAPGLLRKNFGFMHLKGFNRSSWNRMLQEAKARMKRSFQGKLVTTDIDPKAIRASR
                     QNAAAAGVDHLIEFSVRDYAETPVPEGHGVVILNPEYGERMGKVEKLKMIYKGIGDFF
                     KKKCQGYTGYIFTGNLDLAKKVGLKAKRRLPFFSSNIECRLLEYDLYEGSRRISGDET
                     "
     gene            complement(3534..4589)
                     /locus_tag="VEL68_09685"
     CDS             complement(3534..4589)
                     /locus_tag="VEL68_09685"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HXZ36276.1"
                     /translation="MKTPGVPWPFFPTTRNPWIGALSFLLFLAFAPVEGFSDTPRELA
                     ECAKIEDSAERLKCYDNLAGRKSNEPAPALESREKPPSENGKRFSYFSHLWELEPETR
                     RSKYSLSPHRSNYILPYSYNTSPNYEAVRAADPEADLKYEEVTFQLSLKVKVWEDIFS
                     QKADLWVAYTQRSFWQFYNFEDSSPFRETDYEPELLLNLRTDYYLLGLRGRFINVGLN
                     HQSNGQSKPLSRSWNRIVANAGFERGNFSLLLKGWYRIPESVENDDNPNIEDYLGYGE
                     VWGYYFWKGNRFGIMLRNNLHLHTNRGAAQLEWSFPILERIGGYLQYFNGYGESLLDY
                     NHRVNRIGIGFILKDWN"
     gene            complement(4678..4971)
                     /locus_tag="VEL68_09690"
     CDS             complement(4678..4971)
                     /locus_tag="VEL68_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36277.1"
                     /translation="MKLLKKLTDLFPGSPKDSSFWVSVQCGRCGEVIQTRIDLNNDLS
                     VEYGEGEKDSTYFCRKVLIGKKGCYLPIEVELKFNSGRNLIEKKIKGGKFVHA"
BASE COUNT         1141 a         1439 c         1563 g         1159 t
ORIGIN      
        1 gggggtcttt tacgaaacga tcggaaagct ggctgatgtg gaccagcccg tcctggtgca
       61 cgccgatgtc cacgaaggcg ccgaaggcgg tcacgttggt gacgatcccg tccagcttca
      121 tgcccggctg gaggtcttcg atcttttcga ttccctcggc gaagcggaag tcttcgaatt
      181 tctctcgcgg gtcccgtccg ggcttggcca gctccttcag gatgtcgttc agggtcggca
      241 ggccggtctt ctcggtcacg taccttgaca gatcgatctt ccctcgcagc gtcgcttcgc
      301 gcatcaagtc ctccacggta cacccgaggt cttgagccat ggcctgggcc acggcatagc
      361 tttcggggtg aacggcgctg gcatcgagcg gattctttcc cccgcggatt cgcaggaatc
      421 cggcggcctg ctcgaaggcc ttggggccga ggcggggaac cttcttcagc tcatcccggg
      481 aagagaaggg gcccttttcg ttccggtagg cgaggatgtt ggcggcgagc tgcggcccca
      541 gaccggaaac gtaagtgagg agctgggcgc tggtcgtgtt gacctccacc ccgacagcgt
      601 tgacgcagct catgaccacg tcgtccaggc tgcgcttcaa tgcgggctgg tccacgtcat
      661 gctggtactg gcccaccccg atcgatttgg gatcgatctt caccagctcg gccagagggt
      721 ccatgagccg cctcccgatg gaaacagccc cccgaacagt cacatcatga tccgggaatt
      781 cgtcccgggc agcccgggaa gcggaataga tcgaggcgcc gctttcgttg accagcacca
      841 ccgggatccc ggaggggggc ggcaggccgc gaatgaaggc ttcggtctcc cgtccgccgg
      901 tgccgttccc cacggcgacg gcctcaacct ggtactccgc caatagttcc aagactctcc
      961 gagcggattc ctcttttcct ttctcgctga ggaggggaaa gatcgtttcg gtgtgcagga
     1021 gttttccctg gcggtcgagg cagacgacct tgcaccccgt tcgcagcccg ggatctatgg
     1081 ccaggactct tttctggccg agcggggagg ccatcaggag ctgacggagg ttgtcggcga
     1141 agacccggat ggcttcctcg tcggcccgat tcttcgtggt cagtcgggtt tcggtctcca
     1201 tggaagggga gaggaggcgt gcgaagctgt catggacggc cagacggacc tgctgggaac
     1261 aggcattctc ccccttgaca aagaggcgct caaggatggc cagcgcttcg ggctcggggg
     1321 gcgaaatggt gagattcagg aaaccttcct tctccccgcg ccgcatggcc aaaatccggt
     1381 gagaaggagc ctgggaaacc ggctcttccc aggcaaaata atcccggtac ttgcttcctt
     1441 ccgcctcttt acccgcgata accttcgagc gaaagactcc ccgggaggcg aaaaggtccc
     1501 gaagcgcagc ccgggccgcc tgatcttcgc tgacccattc ggcgataata tcccgggcgc
     1561 cagccaacgc gtcctccgca gaggatacgg atttttcagg gtcgatgaaa gggacggcct
     1621 cagacaaggg atccctttcc tcctgggcga agaggatttg agccaggggt tccaaccccc
     1681 tctccctggc caccgtggcc cgggtgcggc gcttgggtcg gaagggaagg tagatgtctt
     1741 ccagaactga cagggtctcc gcggcctgga ttttttcctg gagttcttcg ctgaggaggt
     1801 cccgctcctg gagggatttg aggatggcct ctcgccgctt gtccagctcg accagctgct
     1861 ccagccggtc gcggatggcg gtgatctgca cttcatccag ggaaccggtg atctctttcc
     1921 ggtagcgggc gatgaaaggg atggtgcctc cctcggcgat caatcctgcg gttgcctgga
     1981 cttgtcctgc cgccagcccc agttcggcgg cgatcttcgc tacgtgccgt tcatccatgg
     2041 tttttaggtt ctcttccaat ggaatcattc cgggcggggg tagggtcttt ccacccccga
     2101 gaaaaatctt ctagcaattt ttccggcttt tggcaatgaa ttttcaccgc cgggaatccc
     2161 tcttcaggtc cagtgcctta ggccctgaat ccgtcgatag gcgcacccct tgggcattga
     2221 cccgagaaat tctttttcct atacttgacc ggtagatcag gaggagaaat gacccgttca
     2281 ccctcgccca aaagtcgaat cgtcatcacc tgtgccaaag gagtgcctcc ctttctaaag
     2341 gaggaaatct cgtccctggg ccttccggtc cgatctgaat ccattgccgg ggtggagacc
     2401 gaggggacga tggaggacgc catgaggctg aatcttttcc tgcgcacggg ccagcgggtc
     2461 cttcttttcc tgcgacaact ggaggctaag aacgctgatg agctttacgc tgggatttcg
     2521 cggatggaat gggagaatta tgtgtctccg gaagaatacc tctgcgtcac ttccgccgtg
     2581 gacaatccca ccattcgaga tttccgtttt gccaacctga aatgcaagga tgcgatcgtg
     2641 gaccgcatca agaaaaaggt cggccggcgc cccgactctg gctcgaaaag aaccggggtg
     2701 gtggtcgatc tttactggaa agggaatctt tgcctggttt acctggacac ctcgggggaa
     2761 cccctctacc ggcgcgggta tcggaagatt cccctccatg cccccatgca ggagaccctg
     2821 gccgcggcag tgatccaggc cacgggctgg aaaggccggg gaaattttgt caaccccatg
     2881 tgcggcagcg ggacgctggc cgtggaggcg gccctagccg ccctggatat tgctccggga
     2941 ctgctccgca agaatttcgg gttcatgcac ctgaaaggat ttaaccggtc ctcctggaat
     3001 aggatgctgc aagaggccaa ggctcgtatg aaaagatcat tccaagggaa attggtgacc
     3061 accgacatcg acccaaaggc catccgggct tcgcggcaga atgccgcggc agcaggggtt
     3121 gatcatctga ttgagttttc tgtaagagat tatgccgaga ctccggtacc ggaaggccac
     3181 ggagtggtca ttctcaaccc ggagtatgga gagcgaatgg gaaaggtcga gaaactgaag
     3241 atgatttaca agggaatcgg tgatttcttc aagaagaaat gccaggggta cacaggttat
     3301 atcttcaccg gcaatctgga cctggcgaaa aaggtgggct taaaggccaa acgcaggctc
     3361 ccctttttta gcagcaacat cgaatgccgg ctcctggagt atgacctgta cgaagggagc
     3421 cggaggattt ccggagatga aacctgaccg ggaggaatgg gatcgctgaa atctctcctc
     3481 ccccgggaag ggaaaaaaca aaatgaacgg attcctgaag gaaatccgtc ttctcagttc
     3541 caatccttca agataaaacc tatgccgatc cgattcaccc ggtggttgta gtccagaagg
     3601 ctttcgccat aaccgttgaa atactggaga taccctccga tccgttccaa gatcggaaag
     3661 ctccattcca gctgggcagc gccgcggttg gtatggaggt gaaggttatt gcggagcatg
     3721 atcccgaatc ggttcccttt ccagaagtag tagccccaga cctccccgta tccgaggtaa
     3781 tcttctatgt tcgggttatc gtcattctcg accgattcgg ggatgcggta ccaacctttc
     3841 aagagaagag agaaattgcc cctctcaaag ccggcattgg ccacaatccg gttccagctt
     3901 cgggaaaggg gtttggattg gccattggac tggtgattga gccctacgtt tatgaacctt
     3961 cccctcaaac ccaggaggta gtagtcggtc cgaaggttga gaagaagttc gggctcataa
     4021 tccgtctccc ggaaggggga cgagtcttcg aagttgtaaa attgccagaa ggaccgctgg
     4081 gtgtaagcaa cccataggtc cgccttctgg gaaaaaatgt cttcccagac tttgaccttc
     4141 agactcaact gaaaagtgac ttcttcgtat ttgaggtctg cctccgggtc agcggcccgg
     4201 acggcctcgt aattgggtga ggtgttgtaa gaataaggaa ggatgtagtt ggagcgatga
     4261 ggcgaaaggg agtatttgct gcgccgagtc tccggctcga gctcccagag atgggaaaag
     4321 taggaaaacc ttttcccgtt ttctgatggg ggtttttccc tcgattccaa agcgggcgca
     4381 ggctcgtttg atttacggcc agccaggttg tcgtagcact tcagcctttc cgccgaatcc
     4441 tcgattttgg cgcattcggc gagttcccgg ggagtatccg agaatccctc cacgggggcg
     4501 aaagccagaa aaagcaagaa agacagtgca ccgatccagg gatttcttgt ggtgggaaaa
     4561 aatggccagg gtacgccggg tgttttcatc aggccctttt cctcctgctg ggtatcatcc
     4621 cgtcaaaaat gggtcagagg ccctcgggcg atctccggga agagggaccc ggggaagtca
     4681 cgcatgcaca aactttccgc ccttgatctt cttctcgatg agatttcgac cagaattgaa
     4741 cttcagctcg acctcgatgg gaaggtaaca tcctttcttc ccaataagaa cctttcggca
     4801 aaagtaagtg ctgtcctttt cgccctcacc gtactcgacg ctcaggtcgt tgttcaaatc
     4861 aatccgcgtt tggatgacct cgccgcagcg tccgcactga acggagaccc agaaggaact
     4921 gtctttgggg gagccgggga aaagatcggt gagtttcttt aggagtttca ttgccggaga
     4981 gttcctttcg aagggcagaa tatattatga gagtttatcc acttttggta ttaatttcca
     5041 ggaaatagga agggggaaat ggagaaggta accaatataa aaggaaagga atcggatgag
     5101 ccggattcaa gggagagaag gtagatttca acgcaccgag gatgccgggg agatcgggac
     5161 tctgaaattc gtaaagagga gaactagaga aaaccccagg gcgcacctgc cggtgcgccc
     5221 taagatacat aatttcggaa cgggtcgaat ttcaatctat ttcaatccgt agataaactc
     5281 tctttcaaca taagccagga aa
//
LOCUS       DASPRR010000249         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_373952, whole genome shotgun sequence.
ACCESSION   DASPRR010000249 DASPRR010000000
VERSION     DASPRR010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373952"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VEL68_09695"
     CDS             <1..497
                     /locus_tag="VEL68_09695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ36278.1"
                     /translation="GYWTQFIAKSARSIVATDINPEVFAIARQKEYASCRVRFLESDA
                     YSLSNIETSLTAGFHGFWWSHIPNQKIGAFLDSFHSALPKNALVVMIDNAYVEGSSTP
                     ISRRDQHGNTFQIRQLEDGSKHEVLKNFPSSSELRSRLHSPANYVRVTQLDYYWMVRY
                     RKKG"
     gene            692..1456
                     /locus_tag="VEL68_09700"
     CDS             692..1456
                     /locus_tag="VEL68_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXZ36279.1"
                     /translation="MKPYAITARVTLSLALAVLLVEGAFAHAAEIRVLSTNGVHSVMV
                     EMLPEFERATGHKVSIAYDTANLLLGRIQAGESADLVILTRPALDDLIKQGKVGPGGG
                     KDLARSGVGVAVRAGLPKPDISSADGLKRALLDAKSIAFTKTGASGIHFAKVVERLGI
                     SDQVKAKAKIPAGGSVGELVARGEAEMAVQQIPELLAVPGIQYIGPLPPELQNFTIFT
                     AGVLTSARQAEAARALIDFLSTPAAARVFKAKGMEP"
     gene            complement(1516..2289)
                     /gene="yqeC"
                     /locus_tag="VEL68_09705"
     CDS             complement(1516..2289)
                     /gene="yqeC"
                     /locus_tag="VEL68_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HXZ36280.1"
                     /translation="MTLAEAFQLEDREVISLVGGGGKTTLMFSWGRELANRHRGVILT
                     TTTKIWEPGPSPDFVSMVSSSFIPLREWIFRNLSTHPCLLIARSRLPTGKWEGLPAEW
                     IEEIMKIPGVYCILLEADGAKGHSVKAPREGEPVVPLNTTLLVPVVGIDALGCPLDEN
                     HVFRSKLAMEILQKPEGTILNEELIALLLVATIRRRPDGARVIPFINKIDSPFDLERG
                     RKLGQALFNLPDQEIKRVVLGQAQKTPIVKGVISCPPNP"
     gene            complement(2286..2897)
                     /locus_tag="VEL68_09710"
     CDS             complement(2286..2897)
                     /locus_tag="VEL68_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXZ36281.1"
                     /translation="MFISAIVLAAGESRRMGETKQLLDVGGKPLLQHILDGIRQTKVD
                     EVILVLGHEAERIQKRVSAPGAKVVLNPDYRQGMITSIRQGLQALDPRSEGFFVVLGD
                     QPGIEPKVYNLLIQEFRNCFPRKTILLPTYKGKRGHPALFSVKYRPEAFRIEGDRGFR
                     DVLEMYPQEVFPVELESESIVNDLDTPQDYQNFIGKRAPEEPF"
     gene            complement(2970..>3755)
                     /locus_tag="VEL68_09715"
     CDS             complement(2970..>3755)
                     /locus_tag="VEL68_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HXZ36282.1"
                     /translation="MGDLFDEVQKLKSEGKKGALATIIGTKGSTPREVGAKMLLREDG
                     TLLGTIGGGCLEAEVWQEAMNIIKEQKPRTIHFDLTGKQAEEGGMICGGVMDIYIEPI
                     LPAERVFVFGGGHISLFVAKMAKMAGFEVAVIDDRPQFANPERFPEADEILAEEFFLA
                     LPKLKVNPSSYLVIVTRGHAYDQEVLEWAMETKARYIGMIGSRRKIKTVYDNLAAKGI
                     SQEKLEAVHAPIGLDIGALTPEEIAVAIVAEMIQERRKPKRGR"
BASE COUNT          837 a         1051 c         1031 g          836 t
ORIGIN      
        1 cgggctattg gacgcagttc attgccaagt ccgcaagaag catcgtggct acggacatca
       61 acccggaggt ctttgctatc gcccgccaga aggagtacgc ctcctgccgg gtcaggtttc
      121 ttgaatcgga cgcttactca ctctcgaaca tagaaaccag cttgaccgct gggttccatg
      181 gtttctggtg gtcgcacatc cccaatcaga agataggggc atttctcgat agcttccact
      241 ctgcactgcc aaagaatgct ttggtggtca tgatcgacaa cgcctatgtg gaaggaagca
      301 gcacgcccat ttcgcgaaga gaccaacatg ggaacacatt tcagattaga caacttgagg
      361 acggatcgaa gcacgaagtc ttgaagaact tcccatcgtc gtcggagctg cgcagcagac
      421 tgcactcccc tgccaactat gtccgggtga cgcagctcga ttactactgg atggttcgat
      481 acaggaagaa gggatagagg agccatccca aacacgcggt ggcgtagccg cgaagggggg
      541 ctttatccat tcccttaatg agggaaacca gagggggaat tcaagatgga gaaagtcgag
      601 tggttatttt tcagacgggc aaaaatccgg aggagaaaga gacccgggaa ggggaaagcg
      661 agttcttctc aatgaatagg aggaagacga catgaaacct tacgcaatca ccgccagggt
      721 gaccttaagc ctcgcgctag cagtcttgtt ggttgaagga gcgttcgccc acgcggcaga
      781 gatcagggtg ctttcgacaa acggcgtaca ttcggtcatg gtcgaaatgc tgccggaatt
      841 cgagcgggca acagggcaca aggtctcgat cgcctatgac accgcgaacc ttctgctcgg
      901 acgaatccag gccggagagt cggccgacct ggtcattctg acccggccgg ccctcgacga
      961 tctgatcaag caaggtaagg tcggcccggg aggtggcaag gacttggcga gatcgggggt
     1021 tggtgtcgcg gtacgggcgg ggttgcccaa acccgacatc agctcggccg acggattaaa
     1081 gcgcgccctg cttgacgcaa agtcgatcgc cttcaccaag accggcgcga gcggaattca
     1141 tttcgccaag gtggtcgaac gccttggcat ttcagaccag gtcaaagcca aggctaagat
     1201 acccgccggc ggctcggtgg gggaactcgt cgccaggggg gaagctgaaa tggcggtgca
     1261 gcagatcccc gaactcctgg ctgtgcccgg catacagtat atagggccct tgccaccgga
     1321 gctgcaaaat ttcacaattt tcaccgcagg tgtgctgacc agtgcccgtc aggcggaggc
     1381 ggcgagggcc ctcatcgatt ttctttcgac gccagccgcg gcgcgagtat tcaaggccaa
     1441 gggcatggag ccttgatgct gcagctccag caggccgggg atcggtaagg agactccaag
     1501 gttaagcgga accggctaag ggttcggagg gcaagaaatc actcccttga cgataggggt
     1561 cttttgggcc tggccgagga ccactcgttt aatttcctga tcgggaaggt taaacaatgc
     1621 ctgtcctaat ttccggcccc tttccaagtc gaacggggag tcgattttgt tgataaacgg
     1681 aatcacccgg gccccgtctg gacgcctcct aatggtcgct acaagaagca gggcaatcaa
     1741 ttcttcattt aaaatggttc cttcgggctt ctgcaggatt tccatggcca gtttcgaacg
     1801 aaagacatga ttttcatcca gcgggcatcc cagggcgtcg attccaacga ccggaaccag
     1861 aagagtggta ttcaaaggga ccaccggttc tccttccctc ggcgctttta cggaatggcc
     1921 tttggccccg tccgcctcca aaaggatgca gtagaccccc ggaattttca tgatttcttc
     1981 aatccactcg gcaggaagac cctcccattt accggtgggc agcctggatc gggcgatgag
     2041 caaacaagga tgcgtggata gatttctgaa gatccactct cgcaggggaa tgaaactgga
     2101 agaaaccatg gagacgaaat ccggagaagg ccctggttcc cagatttttg tggtcgtagt
     2161 gaggatcacc cccctatggc gattggcaag ttcacgcccc cacgaaaaca tcagggttgt
     2221 tttgccgcct ccgcccacga ggctgatgac ttcccggtct tccagctgga aagcttccgc
     2281 taaagtcaaa agggttcctc aggagctctc ttaccgatga aattttggta gtcttgggga
     2341 gtgtcgagat cgttcacgat cgattcgctt tccaattcaa ccggaaagac ctcttgagga
     2401 tacatttcca gcacgtcccg gaaacctcta tccccttcga tccgaaatgc ttcaggccga
     2461 tacttgacgc tgaagagggc cgggtgtccc ctttttccct tataggtggg gagtaaaatt
     2521 gtcttgcggg gaaagcagtt ccgaaactcc tggatgagga ggttgtaaac cttcggctcg
     2581 attccgggct ggtcccccaa aacgacgaaa aatccttcgg atcgcgggtc caaagcctgc
     2641 aggccttgcc ggattgaagt aatcattccc tgccgatagt ctggattcaa aaccactttg
     2701 gctccagggg cggagactct cttctgaatc cgctccgcct cgtgtcccag gaccaggatg
     2761 acttcatcca ccttcgtttg gcgaattccg tccaagatat gttgcagaag gggcttcccg
     2821 cccacgtcga gcagttgttt ggtctccccc atccgcctgg attcgccagc agcaagaacg
     2881 atggccgaaa taaacatgtc caccctttca ggtcagatgt acagggcctc ttccatggct
     2941 tgctgggcct cttcgatctg ggcaagcctt caccttcccc ttttgggctt tcttctctcc
     3001 tggatcatct cggccacaat ggccaccgca atctcctcgg gggtgagagc gccgatgtcc
     3061 aggccaatgg gggcatgaac agcctctagt ttttcctgag aaatcccttt ggccgccagg
     3121 ttgtcataaa ccgtcttaat tttcctccgg ctgccgatca tcccgatgta acgggccttt
     3181 gtttccatgg cccattccag gacctcctgg tcataggcat gtccccgggt gacgatgacc
     3241 aggtagctgg atgggttgac ctttaatttt ggcaaagcga ggaagaattc ctcggccaga
     3301 atctcatcag cttccgggaa cctctcgggg ttggcaaact ggggtcgatc atcgatgacc
     3361 gcgacctcga atcccgccat cttggccatc ttggccacga agagagagat atgaccccct
     3421 ccgaatacaa aaacccgttc ggcgggaaga atcggctcga tgtagatatc cataaccccg
     3481 ccgcagatca ttcctccctc ttctgcttgc tttccggtca ggtcaaaatg gatggtacgc
     3541 ggcttttgtt ccttgatgat gttcatggcc tcctgccata cctcggcttc caaacagcca
     3601 cccccgatgg tccccaggag ggtcccgtct tcccggagaa gcattttggc tcccacctcg
     3661 cggggggtcg atcccttggt ccctatgatc gtggccaagg cccctttttt cccttccgat
     3721 ttgagctttt ggacctcatc gaataaatcc cccat
//
LOCUS       DASPRR010000250        19565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_705334, whole genome shotgun sequence.
ACCESSION   DASPRR010000250 DASPRR010000000
VERSION     DASPRR010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19565)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19565
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705334"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..593)
                     /locus_tag="VEL68_09720"
     CDS             complement(3..593)
                     /locus_tag="VEL68_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HXZ36283.1"
                     /translation="MEKNQMIIPIERIDRAIYLIRGQRIMLDADLALLYGVSAGRLNE
                     AVRRNTQRFPADFMFQLTQEEFEELKAQIGRLNLKSQIAISSSGWGGRRHPPYAFTEQ
                     GVAMLSTVLRSDRAIRVNIEIMRAFVRMRQILATNADLERRLSELEKKYDAQFRIVFD
                     ALRQLMAPPAPSRKQIGFEVKERAALYGRKAKKSLG"
     gene            complement(1061..1345)
                     /locus_tag="VEL68_09725"
     CDS             complement(1061..1345)
                     /locus_tag="VEL68_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXZ36284.1"
                     /translation="MSRHPGESRGPEHIEITSGFRLEWQILRDLEKVFLAAGFTFARQ
                     EGSHRSYTKPGILRPVVIPTYEEIPAPIIKNNLKTAGIQREEYFRLLEKV"
     gene            complement(1483..3165)
                     /locus_tag="VEL68_09730"
     CDS             complement(1483..3165)
                     /locus_tag="VEL68_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ36285.1"
                     /translation="MDRLTIPNYVKEKYWLKSYPEGLSWNIVIPAVSLPRFVEEGIKN
                     NPDRVAMVYAGKEIRYSELQARIDRVSCALQDMGVRKGETVALFLSNCPQFACAYYGA
                     LKIGAMITAISPLFMTSEVEFQLRDSRARTLFVQDEFWPRVEPALSSLNLREIVVVNP
                     KGEKPDLAKIPGMFFFGDLLSEGHGSPGPVAIDPLEDVAALQYTGGTTGMPKAAMLTH
                     RNVMANIVQMSGYWDLMARLDGIRHPKVISVLPWYHIYGQTVDLSSTLSQGGTLIVLP
                     GFDAENVLKTIRDTGAHVFMGVSTMFVSFLNHPRLKEYSLKSLKWCNNGATIIPTEVV
                     KNFEEATGVEIVEGYGLSEASPVTHTTSPYLRRKIGSVGPPIPGTLQAVIDPQSGKFL
                     SPGTPGEVIVHGPQVMKGYWQRAKETEEVFIRIEGEKWLRTGDMGVLDEDGYLTIVDR
                     SKDLIKYKGHSVYPSEIEEVLHQHPAVSQAAVVGLPDPLAGETIKAFVILKDDFQGKV
                     REEEIISWTKDRLASYKYPRFLEFRSEIPKSAVGKILRRVLRDEEIKKLGRR"
     gene            complement(3227..4762)
                     /locus_tag="VEL68_09735"
     CDS             complement(3227..4762)
                     /locus_tag="VEL68_09735"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXZ36286.1"
                     /translation="MENNYARYGMEGIKKYGEYVSFIYEEEKFRVEFTNLQIQKKANA
                     LANALKDLGVKPGDVVAVVLPNGPTVPIVFTAIFKMGAVFLPVIFALTAPEVRYILED
                     SRANTIITEESLVPKIEEARQGLRGIRNFIVTNPGGPPSRGLSLEEILGKYPDQFPML
                     PVREDDLAVLMYTSGTTGSPKGVMLSHGNLSSNVEAGAIAIPTRHTDTMLIPLPLNHI
                     FGLLMVNECNLSGARMILHKWFDPQLALRSVTQNRVTEFVGVPTMYIRLMDTFDPAVH
                     SMESIRQFLSGGAPLSAETQMAVEAKWGKPFYQGYGLTECSPTVSRQRVGRPRKPGCV
                     GPAVDKVQIRILDEEDQELPQGEAGEVGVRGPNVMKGYFNQEDATCEAMRGGWLHTGD
                     VGYLDSEGELFITDRKKDLIIRGGENISPGAVEDILFKHPAVLETAVVGIPDPVYGEE
                     VKAFVVLKQDFQASEKELLEHCLKHIPRFKAPKSIGFLKELPKNSVGKVLKRTLREME
                     KKK"
     gene            complement(4774..5463)
                     /locus_tag="VEL68_09740"
     CDS             complement(4774..5463)
                     /locus_tag="VEL68_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36287.1"
                     /translation="MISEKSSVSSSDPVIECQEVNTFRGPVHALRNVSLRVKPGITCI
                     VGPNGAGKTTLLKSIMGLLPVASGKITFLDGEITNRPAHHLAHMGLGYVPEDRRIYPD
                     FSVVENILLGTWRRKKDPEKILLQAYTFFPKLKELSRSKGIHLSGGEQSMLAIARTLA
                     LEPRCLLLDEPLEGLAPVIRKRVAEGISELRAKGITTLTTASDMSYVPDIANVIYRMD
                     RGEIVERQEKG"
     gene            complement(5501..6280)
                     /locus_tag="VEL68_09745"
     CDS             complement(5501..6280)
                     /locus_tag="VEL68_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ36288.1"
                     /translation="MGGQSPAAAPILNTAGVFKHFGEVHAVDGVDLHVSLGEVLSIVG
                     PNGAGKTTLLKLLSGGHELDRGRVFFLGEDISRVSLVQRVNRGIVYSFQIPALFENLT
                     ALEAVGLVILARRKKTVVFFPGMEKFTEIRQEAQEILKMFNVPEDFPANLLPHGQRKL
                     LDAAMAFAYRPKLLLLDEPTSGVSTAEKAKVMDTIMPNIEKSNTTTVIVEHDMEIVLR
                     YSQRVIVMDQGKIFAQGIPREVIEDPKVREVLLGIPASGRT"
     gene            complement(6244..7191)
                     /locus_tag="VEL68_09750"
     CDS             complement(6244..7191)
                     /locus_tag="VEL68_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36289.1"
                     /translation="MLQRFYRTAFIIVLLILITGPVYFPSFVVYLLSYGLIMAIAALA
                     LNLLLGYTGLMSFGHGMFIGAGAYTVALMMKYTGVQSFELIIPLSILVSALLAAVAGL
                     FCTRQIDIYFSMITLAFGELLFALCSKFYSATGGTDGIRVKIPTLFGYSFPGGGEYYY
                     FVLVVFFVCALAIWVICQSPFGKAIQAVRDNEVRVGYIGLVVMKYRLIAFVISGAFTG
                     IAGVLMVSLTGHAVPESFQVFTSISIVFMVLLGGHTTFLGPAVGAIVFVFLQAWLKPL
                     FFWQFFLGGAIILLVMFLPGGVLGGMQLWAAKALQRLRS"
     gene            complement(7210..8088)
                     /locus_tag="VEL68_09755"
     CDS             complement(7210..8088)
                     /locus_tag="VEL68_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36290.1"
                     /translation="MLELAIPLLLNGIFYAGILFLLSAGLQIIFGVLRIVNFAHAAAY
                     ILGTYFSYSIIKALPPEYAFLSWIAPPIAAVLVGGLGLAFERGLLKYIYHRHHVFQIL
                     VTAGIVYMVDDAVRWKWGGYSIGSGPIVEATVELGGVVYPVYNLIIILVALAVAFLLW
                     LIFRKTDIGRVIRATAHDEEITAALGINVSWVKAITFLFGSAIAGLAGGLMLPIIGGW
                     PGVGNEYLILGFVVIALAGLGSVNGALVASLLIGLTNSIGVQFFPKIELAIIFIIMAV
                     VLTVKPSGLFGAQPRR"
     gene            complement(8207..9535)
                     /locus_tag="VEL68_09760"
     CDS             complement(8207..9535)
                     /locus_tag="VEL68_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36291.1"
                     /translation="MGKKGEQVHGGEWGRRDFLKLVGAGAAVTAGNVALSGSLRQAVA
                     QTAPKEPYKIAIVAFTTGPATSYGLPGHEMLDILVPLTNGKGGILGRKVELSKYDEGA
                     VANVVETFKKLTKQDKVDAILGCSSSASALAGAPIAEEAKTIFINCLARTHKVTWAKE
                     DDPTQIRKYVFKSINDTYTHAASAALAVAKFYPKAKVAHIHPDYAYGHEIHEVFNKVI
                     KKVDPKVEFVADLWPKLFAPDFSPHITKLLAAKPTVIFNSFWGGDSTRFTDQCIQKGV
                     MSKAKVLGDHGEFYSVEKRTLPKELIGHPPFPVEGLPGTPDPRRWPLNKIAFDLYFNK
                     HKNTPPVAASFTAAAYYSLVAAIEKAASLVGSWPNNDQIAAAFKGLAVATPLGWHVYR
                     EDNRGVSPKWAGLYKMQPPFSYGIDQIFHLPIEEITAPVGVDPLKWVDTW"
     gene            complement(9719..11044)
                     /locus_tag="VEL68_09765"
     CDS             complement(9719..11044)
                     /locus_tag="VEL68_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36292.1"
                     /translation="MERWLKLGVVFLAVLSLLWLGPSPAAFAQPKGKPIVIGYVGNVA
                     SPGTKPCMDLQKYAVEEINKAGGILGRPVEYVVLDGKGDTSLSVEAARRLLMENKATF
                     ISIEGRSEICLAAQENSAALFKEYPHILVFNGPMASELTARIVDDYEKYKFCFRDWDP
                     EPAHYSWLENIMGGLFKKVLNAKKLAFLWEDLAWTQMWRKGIDYLKLPTWEEVAKQKY
                     GLEVVYSKPVKPRGTMYLPILQEIAMKKADVVWFVSSWFTDTEVYAKQWADSAAKNIP
                     TYLYGGVAQTHDYWRMTGGKSLGMLTGFYEGDIPYTEKTLPFIALGKKLGIPLQIHVQ
                     IAYADIYHFKAAIEKAGGTDDMEKLIKAMEEIETVYSLGKMKYETRRIKPYFHSRVLC
                     DPNNPYYMYPGIYMLPIAQFQKEGKIAWVVDENGKALPKNYIPPANLRK"
     gene            complement(11177..12520)
                     /locus_tag="VEL68_09770"
     CDS             complement(11177..12520)
                     /locus_tag="VEL68_09770"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ36293.1"
                     /translation="MSGKGEKEQVYARYVASGKTIEHLASWGKTLAEVMNPEGKPEVL
                     DDVLVLDASCGNFSGIIAASFLAEFGAEVIKIEPPSGDPMRQVTPYGANVKGVGIPFL
                     VEGRNKKYMILDLRNSDQDRKDFARLAEKAAVVLETFAPGEMDSWGIGYQQLSQKNPG
                     LIYIAIAPYGQYTEKAKAFAHQPDTDITCQAGSGLPAQVGDPANAPEPFNWPLKAGMW
                     AGWYISGLSAALGGTVALVHRQITGEGQMVDVATMDAYSSMVGFPATIGYTWERPRPR
                     IGVLDFILYPYGLWKCKDGYVVIAAPRDHDFRALLKVLGLWHLEDDWRYTADRIPDVV
                     DQAMVLYKEIEEKTLQYTADELVKLALTYSAKSARSKWRGGGVPIVMKMMTPPMTLES
                     KQFAIRRIFQEVEDENAGKFLINTHFVKMSESPPRIKWISSTIGKDNEYVRKKYL"
     gene            complement(12517..13872)
                     /locus_tag="VEL68_09775"
     CDS             complement(12517..13872)
                     /locus_tag="VEL68_09775"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ36294.1"
                     /translation="MEDYFSYTAKLFNPSETNQKPEVLKGIRVLDFSHVIFGPTATRI
                     MANYGAEVIKLELPFHGDLWRPATYWGKYWKHSNPLWHFVTQNKYFVGLDLKHPEAKD
                     IIYRLAQMCDVVAENFAPGTAEAWGVGYTAISKINPRVIYLSCSTYGQYGPLRFYPGW
                     DLLAQAASSVLSLTGYPDTEKFYKLPDYLGDFIPGNFGALAILMALYFRNKTGKGQYI
                     DLAQSEALMRLLSHFTAFKLTGMEMGRTANTDPTMVPASIFKTRDKKYIALACATTKQ
                     FESLCEAMEKEDLLKNPAYTGPFERLKPENAKALTQIVGDWLKGQDLDPVIRLAVEKG
                     FPAAEVQDDYQISQDPWRRQRGSVILFHDEMYGEIVLAGPSAQLSKTPSRTKWTARPV
                     GYHNRLVLKKFLEMSDEQITALEKKKVIGTFDDRPGLKPPVYYNLEEDPVFNYGRGGK
                     K"
     gene            complement(13930..14634)
                     /locus_tag="VEL68_09780"
     CDS             complement(13930..14634)
                     /locus_tag="VEL68_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36295.1"
                     /translation="MLEVKNLNVSYGVIPVLHDVSLRIKENELVALLGSNGAGKSTLL
                     NTIQGFLKPISGSISFFGKALEGLPPHRVIAEGIVQVPEGRRLFPYMTVKENLLLGAY
                     PGNAWASKKERMEWVMGLFPILKERAQMQSRLLSGGEQQMLTIGRGLMALPRLLMVDE
                     PSLGLAPVILDNLYATLGKLRESKITILLPEQNALRALTLGDRGYVMQDGKVVLEGDC
                     QTLMQSDLVKKAYLGR"
     gene            complement(14775..15527)
                     /locus_tag="VEL68_09785"
     CDS             complement(14775..15527)
                     /locus_tag="VEL68_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36296.1"
                     /translation="MPEILTVRNITKSFGGLTAVHDLSFHIRKGETVGLIGPNGAGKT
                     TLVNLLCGSLYADRGEIEMEGKMITRIPPHKRCHLGLSRTYQIPRPFPELTALMSVVT
                     SALCGKNRVNRGLDDAGMEATHYLEFVGLFSKRNILARDLTFFELRMLELARALATSP
                     KLLFVDEVMAGLNPGEATKAVHLIARAKGEFGLTIFWIEHVMAVLMEAAERIIAIHFG
                     EKIAEGTPAEIANNDMVIEAYLGSGWKQGTCN"
     gene            complement(15534..16574)
                     /locus_tag="VEL68_09790"
     CDS             complement(15534..16574)
                     /locus_tag="VEL68_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36297.1"
                     /translation="MVDVEIRRDRIILRYKGKKWDWILEPRYWRNTLLWSAGLLIFPI
                     LCYFISPILLNTVVNANMYAAIAIPLALMTLGTGRINFGPNFFIGVGGYTAALLSIAY
                     GWGPLKTFPFAVLVSLLSALVFSPLVIVARGLYYVLLTLLLPLVFLEITFIYTDTFKG
                     DVGLAGIQLLLNTGMVKVNFLLTAYISMILMLLYLWVINKVIKSRYGLIMATINDGEE
                     VAHGIGVNINKVKILAFVFAAGLIGVAGWFYAHYFGTFAGITYLPLNFMIKILLVIFI
                     GGRAQIFGCVVGGYFIAFLEMILVVTLGEIQPVLFPVILAVLLFTLPEGLFGLYRRRR
                     YREYLPTLHVRR"
     gene            complement(16568..17443)
                     /locus_tag="VEL68_09795"
     CDS             complement(16568..17443)
                     /locus_tag="VEL68_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36298.1"
                     /translation="MWETLQVVLIYTFVLGSIYLLISLGFSIICGVLRIFHLGYAYLF
                     TLTVYLAWMFMKEFGFGLVPALIAMVAIQFGISYLIYKGIIMKYMDREEIILTGLLLV
                     SIIVEQAVNYRYPIQAGVFIQTTLVEGSSDIGSVVISNQLLVGAGIAILLTILFVFFF
                     LKTRTGLAIRALSQDIYSSQIIGVNVEVLYTITMFLILIPVIVGTLIVAPVWAVDPTM
                     GSLYFTTAILVAILGGLGNIKGTIIASYLIGFTHAAVSFVVGEPRFMNFSALVLVMII
                     LMVRPQGIAKSEALW"
     gene            complement(17703..18812)
                     /gene="cobD"
                     /locus_tag="VEL68_09800"
     CDS             complement(17703..18812)
                     /gene="cobD"
                     /locus_tag="VEL68_09800"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HXZ36299.1"
                     /translation="MNKVRGENIYEVAAQQDLDPAKIIDFSASINPLGLSPKAQKRIL
                     KERLAAVLHYPDTRCRELRKALAEYHRIPEDRILPGAGSTEFIYSIPRIMELNRILLV
                     TPAFSEYENALESGERRPKILFFETREEDGFELNVEGLLLALTQGYDALYLCNPNSPT
                     GILAEKKDLLRVLAQAQREKVWFILDEAFIDFVPGESLVREAASSSRLIILRSLTHFF
                     AMPGLRAGYMVCNPEVSRKFEEEKGPWAVNALAQIAAVESLRDKTYIERTLASNQAER
                     ENLTLGLQGIPGFIPYPGSANYLLVQLHPSLNLTAAELREKLIPRGILIRDCQSFHHL
                     GPYFFRVSVRTRKENQALLKLLRQVHKEMLRGKTG"
     gene            complement(19090..19365)
                     /locus_tag="VEL68_09805"
     CDS             complement(19090..19365)
                     /locus_tag="VEL68_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36300.1"
                     /translation="MEWVFEDIEVESYSGYKGEESLRAFVHLGKRFEVLEILDRWYEG
                     GLDPKALRHDYFRVKTKEGETFMLRYTPRFQAWTLCRRLPAPRVSNN"
BASE COUNT         4219 a         5517 c         5424 g         4405 t
ORIGIN      
        1 ttttatccca aggatttctt cgccttccta ccatataatg ccgctctctc cttcacctca
       61 aaaccgattt gtttgcgcga tggggctggt ggagccatga gttgacggag ggcatcgaaa
      121 acgatgcgaa actgggcgtc atatttcttt tctagttcgc tgagcctacg ctctaaatct
      181 gcattggtgg ccagaatttg gcgcattcgc acaaaagctc tcataatttc tatgttcacc
      241 cgaatagcac ggtcacttcg caggaccgtg gagagcattg caactccttg ttcggtaaaa
      301 gcataagggg ggtgacgcct acctccccaa cctgaacttg atatcgcaat ttgcgatttc
      361 aagtttaacc ttccaatttg ggctttcaat tcctcaaatt cttcttgggt gagctggaac
      421 ataaaatcag ccgggaaccg ctgggtattc cgtctaacgg cttcgttaag cctcccggca
      481 ctgacaccat aaagcaatgc caaatccgca tctaacataa tcctctgccc tcgaatcaga
      541 tagatcgccc ggtcaatccg ttcaatggga ataatcattt ggttcttttc catggtaccc
      601 cttttcgatt ccatatgaat ttatctttcg caaaaacctc atgattccgg gggaaaaacc
      661 ccatcgaatt cgttcgctct ggtgccaggc ctcatatcag aaccatgtcc ccagatatat
      721 cgtagatacc ggcggcctct tcttcttttt gacatcacaa gttgtgatct caaaagttgg
      781 aattcgtcgt atgtcatttg gaacatagca attagcaacg agcccaaagc aaaaaagcac
      841 tgctcgacct tcgatcggta cagtgcttct ttggtttgcc tttccaatcg accacccccg
      901 atgggctaaa ggcaatttcc tggtttttct cttttgggga cagaaggggg attttatttc
      961 gggaatctca ccccccagca ttcaattccc cgcagacagc agagatatac caaattttat
     1021 gagggaagat caagtttcag ggagattagc tacacgttgc ctaaactttt tcaagcagtc
     1081 gaaaatattc ttctcgttgg attcccgctg ttttcaaatt attcttaatg atcggagcgg
     1141 ggatttcttc gtaggtggga atgacgacag gacgcagaat cccaggtttt gtataggatc
     1201 gatggcttcc ttcctgccgg gcaaatgtga agcctgcggc aagaaaaact ttctccagat
     1261 ccctcaaaat ttgccattcc agtcgaaagc cggaagttat ttcaatatgt tctggacccc
     1321 ggctttcgcc ggggtgacgg ctcaaaagac tttttacgag atcatcaaat tttagccatg
     1381 gaataccatt tcaaaaagct tctcagcaag atgaatcgcc tcttccgctg aaaaaggagg
     1441 attaaggggg atttgatttg atagcattct ggaaatctgc gtttatctgc gtcccaattt
     1501 ttttatttcc tcgtccctta ggactcgccg cagaattttg ccaaccgcgc tcttggggat
     1561 ttcgctccgg aattcgagaa accggggata tttgtagctg gccagccggt ccttcgtcca
     1621 ggatatgatc tcctcctccc gaaccttccc ctgaaaatca tccttcaaaa tgacgaaggc
     1681 tttgatggtc tcccccgcca gggggtcggg gagcccgacc accgcggctt ggctcaccgc
     1741 cgggtgctgg tgcaggactt cttcaatctc gctcgggtat acggaatgac ctttgtactt
     1801 gatcaggtcc ttggagcggt ccacgatggt caagtagccg tcttcatcca ggactcccat
     1861 gtccccggtc cgcagccatt tctccccttc gatccggata aagacttcct ccgtttcctt
     1921 cgctctctgc cagtaccctt tcatcacctg cgggccgtgg acgatgactt ctcccggcgt
     1981 tcccggagaa agaaattttc cgctctgcgg gtcgatgact gcctgcaggg taccggggat
     2041 tgggggtccc accgacccga tcttgcgccg gaggtagggg gaggtggtat gggtcaccgg
     2101 gctggcttcg gaaagcccgt atccctccac aatctccacc ccggtggcct cctcgaagtt
     2161 tttgaccacc tccgtgggaa tgatggtggc tccgttgttg caccatttca gggatttcag
     2221 gctgtattcc ttcagccggg gatggttcag aaaggagacg aacatagttg agacccccat
     2281 gaacacatgg gctcccgtat cccggatggt cttcaggaca ttctccgcat caaagccggg
     2341 aaggacgatg agagtccccc cctgggaaag ggtggaggaa aggtccaccg tttggccata
     2401 aatgtgatac cagggaagga cgctgatgac cttggggtgg cggatcccgt ccagtcgggc
     2461 catcaggtcc cagtagcccg acatctggac aatgttggcc attacgttcc ggtgggttag
     2521 catcgcagcc ttaggcatgc cggtggttcc ccccgtatac tgcagggcag ccacatcctc
     2581 caaaggatcg atggccacgg ggcccgggga gccgtgacct tcgcttaaga ggtctccgaa
     2641 gaaaaacatc cccgggatct tcgccaggtc tggcttctct cctttcggat tcaccacaac
     2701 gatttcccgc aggttcaggc tagagagagc cggctccacc ctgggccaga attcatcctg
     2761 tacgaagagg gtccgggctc tactgtcccg aagctgaaac tccacctcgg aagtcatgaa
     2821 gagaggactg atcgccgtga tcatagcccc gattttaagg gccccatagt aagcgcacgc
     2881 aaactggggg cagttggaaa ggaaaagagc cactgtttca cctttccgca ctcccatgtc
     2941 ctgaagggcg caggaaacgc ggtcgatcct ggcttgcagt tccgaatacc ggatctcttt
     3001 tccggcatag accatggcaa cccgatcggg attgttcttt ataccctcct caacaaatcg
     3061 aggaagagag acggcgggaa tgacaatatt ccaggagagt ccctccgggt aggacttcag
     3121 ccagtatttt tccttcacat aatttgggat agtcaagcga tccatccctt ccgggccagg
     3181 gggccaatcg ggtccatttc acccaaaggg ggtaaatatg gatcctctat ttcttctttt
     3241 ccatttcccg cagcgttcgt ttcaggacct tgcccactga attcttggga agctccttca
     3301 ggaagccgat ggacttaggg gctttgaagc ggggaatatg cttcaggcaa tgctcgagaa
     3361 gttccttctc ggaagcctgg aaatcctgct ttaagaccac gaaggccttc acttcttctc
     3421 cgtaaacggg atcggggatt cccaccacgg cggtttccaa gaccgccggg tgcttgaaga
     3481 gaatgtcttc gacggcccca ggggaaatgt tctccccgcc gcggatgatc aggtctttct
     3541 tccggtcggt gatgaagagc tccccctctg aatcgaggta tcccacgtct ccggtatgca
     3601 gccacccgcc cctcatggcc tcgcaggttg catcttcttg gttgaaatac cccttcatca
     3661 cgttggggcc gcggacaccc acttcccccg cctccccctg ggggagttcc tggtcctctt
     3721 catccaggat tctgatctgc accttgtcca ccgccggtcc cacgcacccg ggttttctcg
     3781 gtcgacccac cctctggcgg gagaccgtgg gggaacattc ggtcaggccg tacccctggt
     3841 agaagggctt cccccatttg gcctccaccg ccatttgggt ctcggcactc agcggcgccc
     3901 cgccgctgag gaattggcgg atggactcca tgctgtgaac cgcggggtca aaggtatcca
     3961 tcaaccggat gtacatggtg ggaacgccca cgaattcggt cactcgattc tgagtgacgc
     4021 tgcgaagggc cagctgggga tcgaaccatt tgtggaggat catcctcgcc ccggaaaggt
     4081 tgcactcgtt gaccatgagg aggccgaaga tatggttcag gggaagggga atcaacatcg
     4141 tgtcggtgtg ccgggtggga atggcgatgg cccccgcttc cacgttggag ctgaggttgc
     4201 cgtggctcag catgaccccc tttggggagc ccgtagtccc cgaggtgtac atcaggaccg
     4261 ccaggtcatc ctcccggacc gggagcatgg ggaattggtc ggggtattta cccagaatct
     4321 cttccaggga gaggccccgg ctagggggcc cgccgggatt cgtcacgatg aaattccgaa
     4381 tccctctcag accctggcgc gcttcttcga tcttgggaac caggctctcc tcggtgatga
     4441 tcgtattcgc tcgggagtct tccagaatat accggacctc gggggcggtg agggcaaaga
     4501 tgaccgggag gaaaaccgcc cccattttga agatcgccgt gaagactatc ggaaccgttg
     4561 gcccgttggg aagaacgacc gccaccacgt ccccgggctt tacccccaag tctttcagcg
     4621 cattggccag ggcgttggcc ttcttctgga tctggaggtt ggtgaactcc acccgaaact
     4681 tttcttcctc atagatgaaa gaaacgtatt caccgtactt cttgattccc tccatcccgt
     4741 accgggcgta attgttctcc atctccccct ctcctatccc ttttcctgcc gctccacgat
     4801 ctccccccgg tccatgcggt aaatgacatt ggcgatgtcc gggacataag acatatccga
     4861 ggccgtggtc aaagtggtga tccccttcgc ccgaagttcg ctgatcccct cggccacccg
     4921 tttgcggatc accggggcca gcccttccag gggttcgtcc agcagaaggc aacggggctc
     4981 caacgccagg gtccgggcaa tggccagcat gctctgttct cccccgctga ggtggatccc
     5041 cttggaccgg gacagctcct tcagcttggg gaaaaaggtg taggcctgca ggaggatttt
     5101 ctccgggtct tttttccgac gccacgttcc gaggagaatg ttctccacta cggaaaagtc
     5161 cggatagatc cgccggtctt ccggaacata cccaaggccc atatgggcca ggtgatgggc
     5221 cggccgattg gttatctccc cgtccaaaaa ggtgatcttt cctgaggcga cgggcagaag
     5281 gcccatgatg gacttcagca gagtcgtctt ccctgcgccg ttgggaccca cgatgcaggt
     5341 gatccccggc ttcacccgca gagaaacgtt ccgcagggcg tgcaccggtc cccggaaggt
     5401 attcacctct tggcactcga tcaccggatc ggaggaagaa acggagcttt tttcagaaat
     5461 catcttctta tcatccttcg ttcccataga cgggcttcgc tcaggttctc cccgaagccg
     5521 gaatccccag caggacttcc cggaccttgg ggtcctcaat gacctcccgt gggataccct
     5581 gggcgaaaat ctttccctgg tccatgacga tcacccgctg cgaatagcgc agcacgatct
     5641 ccatgtcatg ctccacgatg accgtggtgg tattgctctt ctcgatgttg ggcatgatcg
     5701 tgtccatgac cttggctttc tccgccgtgc tgacccccga ggtgggttca tccaggagca
     5761 ggagcttcgg ccggtaggcg aaggccatgg ccgcgtccag aagcttcctc tggccatggg
     5821 ggaggaggtt ggccggaaaa tcctcgggga cgttgaacat tttcaggatc tcctgggctt
     5881 cctgccggat ttcggtaaat ttctccatgc ccgggaagaa aaccacggtc ttcttcctcc
     5941 gggccaggat gaccaggccc accgcttcca gggcggtcag gttttcgaaa agagccggga
     6001 tctgaaaaga gtaaacgatt ccccggttca cccgctgaac caggctgacc cggctgatat
     6061 cctctcccag gaaaaagacc cggccccggt ccagctcatg ccccccgctg aggagcttca
     6121 ataaagttgt ctttcccgcg ccgttcggcc ctacgattga aaggacttcc cccagcgaaa
     6181 catgaaggtc caccccgtcc accgcgtgga cctccccgaa gtgtttgaaa acgccggccg
     6241 tattcaggat cggagccgct gcagggcttt ggccgcccat agttgcattc cccccaaaac
     6301 tccccccgga agaaacatga ccaggagaat aatcgccccc cccagaaaga actgccagaa
     6361 gaaaagaggc ttgagccagg cctgcaggaa tacgaagacg atggctccca cggccggccc
     6421 cagaaaagtc gtgtgccccc ccaggaggac catgaaaaca atggagatgg aagtgaagac
     6481 ctggaaggac tccggcacgg catgcccggt cagggagacc atgaggactc ccgcgattcc
     6541 ggtgaaggcc ccggatatga cgaaggcgat gagccgatac ttcatcacca ccagcccgat
     6601 atagccgacc cggacttcgt tatcccgcac cgcctggatg gccttcccga agggggactg
     6661 acagatcacc cagatggcca gggcgcagac gaagaagaca accaggacaa aatagtaata
     6721 ctccccgccc cccggaaagg agtagccgaa gagggtggga atcttcaccc ggatcccgtc
     6781 cgttcccccg gtagccgagt aaaacttcga gcacagggcg aaaaggagct ccccgaaggc
     6841 cagggtgatc atggaaaagt agatgtcgat ctgccgggtg cagaacaacc cggccacggc
     6901 agccaggagg gcggagacga ggatgctcaa gggaatgatc agttcgaagg attggacccc
     6961 ggtgtatttc atcatcaggg ccaccgtgta ggccccggcg ccgatgaaca tgccgtggcc
     7021 gaaagacata agccccgtat acccgagcag aaggttgagg gccagggcgg cgatggccat
     7081 gatgaggccg taggagagaa gatagacgac gaaactcgga aaataaaccg gtccggtgat
     7141 caggatcagg agaacaatga tgaaggccgt ccgatagaac cgttgcagca tgcaagactc
     7201 cctttcccct caccttcggg gctgggcccc gaaaagcccg ctgggcttca cggtcaggac
     7261 cacggccatg atgatgaaga tgatggccag ctcgatcttg gggaagaact gtactcctat
     7321 ggagttggtc aggccgatga gcagggacgc caccagggcc ccgttcacgc tgcccagccc
     7381 ggccagggcg ataactacga agcctaggat gaggtattcg tttcccactc cgggccatcc
     7441 tccgatgatg gggagcatca atccccccgc cagcccggcg atggccgaac cgaagagaaa
     7501 ggtgatcgcc ttgacccagc tcacgttgat cccgagggcc gcggtgatct cttcatcgtg
     7561 ggcggtggcc cggatgaccc ggccgatgtc cgttttccgg aagatcagcc agaggagaaa
     7621 agctacggcc agagccacca ggatgatgat caggttgtac accgggtaga ccactcctcc
     7681 caattccacg gtcgcttcca caatcggccc gctcccgatg gagtaccctc cccacttcca
     7741 gcgaaccgcg tcatcgacca tgtatacgat gcccgcggtc accaggatct ggaacacatg
     7801 gtgtcggtgg taaatgtact taagaaggcc ccgctcgaag gccaggccca aacccccgac
     7861 caggaccgcc gcgatcggag gcgcgatcca ggatagaaag gcgtattcgg gcggaagggc
     7921 tttgataatg gaataggaga aatacgtacc caggatgtaa gcggcggcgt gagcgaagtt
     7981 tacgatccgg agcacgccga agataatctg caggccggcg gaaaggagga acaggatgcc
     8041 tgcatagaag atgccgttta gcagtaaagg aatcgctaat tccagcaagg tttctcccca
     8101 aaacccatcc cccccgcccg gcgaagagag atcgggcagg ggggaatcag gaaggtgaat
     8161 tttcgccttt atttcgttga cccgggacgg aaacggaccc cggtgattac caggtatcca
     8221 cccatttgag ggggtccacc cctaccggag cggtgatctc ctcgatgggc aggtggaaaa
     8281 tctggtcgat gccgtaggag aaggggggct gcattttgta caggccggcc cacttggggg
     8341 agaccccgcg gttatcctcc cggtatacat gccatcccag gggcgtggct acggccaatc
     8401 ctttgaaggc tgcggcaatc tgatcgttat tgggccagct gccgaccagg gatgccgctt
     8461 tttcaatggc tgcaaccaag ctgtaatagg cagccgccgt gaaagaggct gccacgggcg
     8521 gcgtgttctt gtgcttattg aaatacagat cgaaagcaat cttgttcaag ggccatctcc
     8581 tcgggtccgg ggttccgggg agaccctcca cggggaaggg gggatgtcca atgagttcct
     8641 tcggaagagt gcgcttctcc acggagtaga actctccatg gtcccccagg accttggcct
     8701 tgctcatgac ccctttctgg atacactggt ccgtgaatcg ggtggaatcc cctccccaga
     8761 agctgttgaa gatgaccgta ggtttcgccg ccagtaattt ggtaatgtgg ggtgaaaaat
     8821 ccggggcgaa gagcttgggc cacaggtcgg ccacaaattc taccttggga tcgaccttct
     8881 tgatcacctt gttgaatacc tcatggatct catgtccgta ggcgtaatcc gggtgaatat
     8941 gggccacctt ggccttgggg tagaatttgg ccaccgccag ggccgccgat gcggcatggg
     9001 tgtaggtgtc gttgatcgac ttaaagacat acttccggat ttgggtggga tcatcctctt
     9061 tggcccaggt caccttgtgg gtccgggcca ggcagttgat gaaaatggtc ttggcctctt
     9121 cagcgatggg ggccccggcc agggcgctgg cgctggaaga acaacccaaa atggcgtcta
     9181 ccttgtcctg cttggtgagc ttcttgaagg tctccaccac gttggccacc gcgccctcgt
     9241 cgtattttga gagttcgacc ttacgcccaa ggatgccgcc cttgccgttg gtcaggggca
     9301 ccaggatgtc cagcatctcg tgccccggca gcccgtagga cgtggccggg ccagtggtga
     9361 aagccacgat ggcgatcttg tagggttctt tcggtgccgt ctgggccacc gcctgtcgca
     9421 gtgacccgct gagcgccacg ttccccgcgg tcacggcggc acctgccccc acgagcttca
     9481 agaaatccct gcgtccccac tctcccccgt gaacctgttc ccctttcttt cccatctcca
     9541 gacctccttt tcttccttcc tccgccgacg ggagggaagg atggatttag gtatgagtgt
     9601 cctctcatat cattttttat tcggacctgt caaagggtat ctgggcctga aaaattttgg
     9661 agaaaaaaaa ggcgggcggc aaattccatg ccgcccgcct tcttcaacga tggattcgct
     9721 acttcctcaa attcgcggga gggatgtaat ttttggggag cgctttcccg ttttcatcaa
     9781 ccacccacgc gattttccct tctttctgga actgggcaat gggcaacatg tagatcccgg
     9841 gatacatata ataaggatta ttgggatcgc acaggacccg ggagtggaaa tacggcttaa
     9901 tgcgccgggt ttcgtatttc atttttccca gggagtaaac cgtctcgatc tcttccatgg
     9961 ctttgattag cttttccatg tcatccgttc ccccggcctt ctcgatggcc gccttgaagt
    10021 ggtagatgtc ggcgtaggct atctggacat gaatctggag cgggattccc agctttttcc
    10081 ccagggcgat aaagggaaga gtcttttccg tgtaaggaat atcgccttcg tagaatccgg
    10141 tgagcattcc cagcgacttc ccaccggtca tcctccaata atcatgcgtc tgggcgaccc
    10201 ctccgtataa ataggtaggg atgtttttgg ccgccgaatc agcccactgc ttggcgtaaa
    10261 cctccgtgtc ggtgaaccag gagctcacaa accagaccac gtcggccttc ttcatggcga
    10321 tttcctgcag gatcggcaaa tacatggttc cccttggttt caccggcttg ctgtacacca
    10381 cttccaggcc gtatttttgc ttagccacct cttcccacgt gggaagttta aggtaatcaa
    10441 ttcctttgcg ccacatctgg gtccaggcca ggtcttccca caggaaagca agctttttag
    10501 cgtttaggac ctttttgaac aagcccccca tgatgttttc aagccaggag taatgggcgg
    10561 gctctggatc ccagtcccgg aagcagaatt tgtatttttc ataatcatcc acaatccgag
    10621 cggtcaactc ggaggccata ggaccgttga agactaggat atgaggatat tccttaaaaa
    10681 gagcggctga gttttcctgc gccgccagac aaatttcgga cctcccttcg atcgagatga
    10741 aggtggcctt attttccatg agcagtctcc gggctgcttc aacggagaga gaagtgtccc
    10801 ccttgccgtc gagaaccaca tactcaactg gtcggcccaa aatcccgccg gctttgttga
    10861 tctcctccac cgcgtacttc tggaggtcca tgcagggttt agtcccaggg gaggcgacgt
    10921 tgccgacata gccaatgacg attggcttgc ctttcggctg ggcaaaggca gcggggctgg
    10981 gtcccagcca caagagcgaa aggaccgcca gaaaaacgac tcccaactta agccatcttt
    11041 ccattgctta ccctccttgt tcgttatttt ttgaggccct tggtcctcag acaaccaaga
    11101 atcagaattc agaatccagg agccagaagt cagaaaggat tcttttcttc tggcttctta
    11161 ctcctggatt ctgcctttac agatacttct tacggacata ctcgttgtct ttcccgatgg
    11221 tgctggagat ccatttgatc cggggcgggg attcggacat ttttacgaag tgggtgttga
    11281 ttaagaattt gcccgcgttc tcgtcctcca cctcttggaa gatcctgcgg atggcgaact
    11341 gcttgctttc cagggtcatg ggcggggtca tcatcttcat gacgatgggg actccccctc
    11401 ctctccactt ggaccgggcg gacttggcgc tataggtcag ggccagcttc accagttcgt
    11461 cggcggtgta ctgaagggtc ttctcttcga tttccttgta taacaccata gcctggtcca
    11521 ccacgtcggg aatgcggtcg gccgtgtatc tccaatcgtc ttctagatgc cagagcccga
    11581 gaactttgag cagggcccga aaatcgtgat cccggggagc ggcaatcacc acatagccat
    11641 ccttgcattt ccaaagccca taaggataaa ggatgaaatc gaggacccca atccgcggcc
    11701 ggggcctttc ccaggtgtag ccgatggtgg ccggaaaccc aaccatgctg gagtaggcat
    11761 ccatggtggc tacgtccacc atctggcctt ctccggttat ttgccgatga acaagggcta
    11821 cggttccgcc cagggcggcg ctcaaacccg aaatgtacca gccagcccac atgccggctt
    11881 tcagcggcca gttgaagggt tcaggagcat tcgccgggtc tcctacctgg gcaggaaggc
    11941 ccgaaccggc ctggcaggta atgtccgtat ccggctgatg ggcgaacgct ttggccttct
    12001 ccgtatactg gccataaggt gcaatggcaa tataaataag ccccgggttt ttctggctca
    12061 gctgctggta cccgatcccc caggaatcca tctcccccgg ggcaaaggtc tcgaggacca
    12121 ccgcagcctt ttccgccagc cgggcaaagt ccttgcggtc ctggtccgaa tttctaaggt
    12181 ccagaatcat gtatttttta ttgcgcccct cgaccagaaa cgggatcccc acccctttga
    12241 cgtttgctcc gtagggggta acctggcgca tggggtcccc agagggcggt tcaatcttga
    12301 tgacttccgc gccgaattcg gccaggaaac tggcggcgat gatcccgctg aaatttccgc
    12361 agcttgcgtc cagaaccagg acgtcgtcca ggacttcggg tttcccttcc gggttcatca
    12421 cctcggccag ggtttttccc caagaggcca ggtgctcgat ggtctttccc gaagctacat
    12481 aacgggcgta tacctgctct ttttcccctt tcccgctcac ttctttcccc ctcttccgta
    12541 gttaaagacg ggatcctcct ccaggttgta gtaaaccggg ggcttgagcc cggggcggtc
    12601 gtcaaaggtt ccgataacct tctttttctc cagggccgtg atctgttcgt cggacatttc
    12661 cagaaacttt ttcaagacca gacggttgtg gtaacccacg ggccgggcgg tccacttggt
    12721 ccgcgagggc gtcttgctca actgggcgga agggccggcc aggacgatct ctccatacat
    12781 ttcatcatgg aagagaatca cgctgcccct ctggcgccgc caggggtcct gggaaatttg
    12841 atagtcgtcc tgcacctcgg ccgcgggaaa ccctttctca acggccaggc ggatcactgg
    12901 atcgaggtcc tgccccttca gccagtcccc tacgatctgg gtcaaggctt tggcgttttc
    12961 cggcttcagc ctctcgaacg ggccggtgta ggcggggttc ttcaggagat cctctttttc
    13021 catggcctcg cagagggact caaattgttt ggttgtggcg caggccaggg cgatatactt
    13081 cttgtcccgg gtcttgaaga tggaagccgg caccatggtg ggatcggtgt tggccgtccg
    13141 ccccatctcc attcccgtca gtttgaaggc cgtgaagtgg gacaggagtc gcatcagggc
    13201 ttcggattgg gccaggtcga tatattggcc cttcccggtc ttgttgcgga agtaaagggc
    13261 catgaggatg gccagggccc cgaaattccc ggggatgaag tcgccgaggt agtccggcag
    13321 tttgtagaac ttttctgtat ccggataacc cgtaaggctc agcacgctgc tggccgcctg
    13381 ggccaggagg tcccagccgg ggtagaagcg cagggggccg tattgcccgt aggtggagca
    13441 gctcaggtag atgaccctgg ggttgatctt gctgattgcc gtgtagccca ccccccacgc
    13501 ctcggccgtg cccggggcaa agttttccgc taccacatcg cacatctggg ccagccggta
    13561 aatgatgtcc ttggcttcgg ggtgcttcag gtccagcccg acaaaatatt tattctgggt
    13621 cacgaaatgc cagagagggt tggaatgctt ccagtacttc ccccagtagg tggccggcct
    13681 ccacaggtct ccatgaaagg ggagctccag cttaatcacc tcagctccat aattggccat
    13741 gatccgggtg gcggtgggac cgaagatgac gtgggaaaaa tccaggaccc ggattccctt
    13801 caggacctcg ggcttctggt tggtctccga gggattgaag agcttggccg tataggaaaa
    13861 ataatcttcc atatcccatc ctcttggctt ttgtcttttt ctttcgcttt gctctctgcc
    13921 ctatgcgcct tacctaccca ggtaggcctt tttcaccaag tcgctttgca tcagggtctg
    13981 gcaatcccct tccaggacaa cttttccgtc ctgcatcaca tagccccggt cgccgagggt
    14041 cagggcccgc agggcgttct gttcgggaag gagaatggtg atcttggact cccgcagttt
    14101 tcccagggtg gcgtataggt tgtccagaat cacgggggcc aaccccagag acggctcatc
    14161 caccatgagc agccggggaa gggccattaa tccacggccg atagtcagca tctgctgttc
    14221 tcccccgctg agaaggcgcg attgcatctg ggcccgctcc ttcagaatgg gaaacaggcc
    14281 catgacccat tccattctct ctttcttgct ggcccaggcg tttcccggat aggctccgag
    14341 cagaagattc tctttcacag tcatgtaagg gaaaaggcgt cgtccttcgg gaacctgaac
    14401 gatcccttcg gcaatcaccc gatggggagg aagtccttcc agcgcttttc caaagaacga
    14461 gatggaaccg gaaatcggtt tcaggaatcc ctgaatggtg tttaaaaggg tgcttttccc
    14521 cgccccattc gaccccagaa gcgccaccag ctcattctct ttgatccgca agctcacgtc
    14581 gtgcaggacc ggaatgaccc cgtaggatac gttcaggttt tttacctcaa gcatgattgc
    14641 ctcattggag aaaccgaata gaaacctgta ggggcaaccc tccgtggttg cccggaatca
    14701 gggcggccac agggggccgc ccctaccttc tttctttttg gctcttcaat ttccgcgtcc
    14761 tgctccttgc gctttcaatt acacgttccc tgcttccagc ccgacccgag gtaggcctcg
    14821 ataaccatgt cgttattggc aatctctgcc ggagtcccct cggcgatctt ctccccgaaa
    14881 tggatggcga tgatccgttc cgctgcttcc atcaggacgg ccatgacgtg ttcgatccag
    14941 aagatggtca ggccgaattc tcccttggcc cgggcgatca ggtgaaccgc cttggtagcc
    15001 tcccccgggt tgagcccggc catgacctcg tctacgaaga gaagtttggg ggaggtggcc
    15061 agcgcccggg ccagttccag catccggagt tcaaagaagg taaggtcccg ggccaggatg
    15121 ttccgtttgc tgaagagtcc cacaaattca aggtagtgag tcgcttccat gccagcgtcg
    15181 tccaggcccc ggttgacccg gtttttgccg caaagggcgc tggtgaccac gctcatcagg
    15241 gcggtgagtt cgggaaaggg ccgaggaatc tggtacgtcc ggctgaggcc cagatggcag
    15301 cgtttgtgcg ggggaatgcg ggtgatcatc ttcccttcca tttcgatctc accccggtcg
    15361 gcatacaggg acccgcacag gaggttcacc agggtggtct ttccggctcc gttcggcccg
    15421 atgaggccca ctgtctcccc tttccggatg tggaagctca aatcgtggac ggcggtcagc
    15481 cctccgaagg atttggtaat attccggacc gtgagaatct caggcatctt ggtctacctc
    15541 cgcacatgca gggttggcag atattcccgg tagcgccgtc tccggtacag cccgaagagg
    15601 ccctcgggga gggtgaaaag gagaaccgcc aggatgaccg gaaagaggac cggctggatc
    15661 tcccccaggg tgaccaccag gatcatctcc aggaaggcga tgaaatagcc ccccaccaca
    15721 catccgaata tttgggcccg acccccgatg aagatgacca ggagaatctt gatcatgaaa
    15781 ttcagcggaa gataggtgat tccggcaaag gtcccgaaat aatgggcata gaaccaccct
    15841 gcgactccga tcaaccccgc ggcaaaaacg aaagccagga tcttgacctt gttgatgttg
    15901 accccgattc catgggccac ctcctccccg tcattgatcg tggccatgat gaggccgtac
    15961 cgggatttga tgaccttgtt aatcacccat aaataaagga gcatcaggat catggagata
    16021 taagccgtaa ggagaaaatt gaccttcacc attcccgtgt tcaacagtag ctggatcccc
    16081 gccaggccca cgtccccctt gaaggtgtcg gtgtagatga aggtgatttc caggaagacc
    16141 aggggcagaa gcagggtcag gagtacataa tagaggcccc gggccacaat caccaggggg
    16201 gaaaagacca gagccgaaag gagactcacg aggacggcga aggggaaggt tttcagagga
    16261 ccccacccgt aggcgatgga gagaagggct gccgtgtatc ctcccacgcc aatgaaaaaa
    16321 ttcggaccga agttaatccg cccggtcccc agggtcatga gcgccagggg gatggcgatg
    16381 gctgcgtaca tgttggcgtt gaccaccgtg ttgaggagga tggggctgat gaagtaacaa
    16441 aggatgggaa agatcaggag gcccgccgac cagaggaggg tattccgcca gtagcggggc
    16501 tccaggatcc agtcccactt ttttcctttg taacggagga tgatccggtc tcgcctgatt
    16561 tctacatcta ccatagagcc tcgctcttgg cgattccttg gggtctcacc attagaatga
    16621 tcatgaccag aaccaaagcc gagaaattca tgaaccgggg ctctcccacc acgaaagata
    16681 ccgcggcatg ggtgaaaccg atgaggtaac tcgcgatgat ggttcccttg atgtttccca
    16741 gccctcccag gatggccacc aggatggccg tggtaaagta aagggatccc atggtggggt
    16801 ccacagccca gaccggggcc acgatgagag tccccacgat gaccggaatc aggatgagga
    16861 acatggtaat ggtgtaaaga acctctacgt tgaccccgat gatttgactg ctgtagatat
    16921 cctgggacag ggcccggatg gccaagccgg tccgggtctt caggaagaag aagacgaaga
    16981 ggatggtgag tagaatggcg attccggcgc cgacgagcag ctggttggaa atcaccaccg
    17041 atccgatgtc cgagctcccc tcgacaagcg tcgtctggat gaaaaccccg gcctgaatgg
    17101 ggtagcggta gttgaccgcc tgttccacga tgatggacac gagtaggagt ccggtcagga
    17161 tgatctcttc ccggtccatg tatttcatga tgatcccctt gtagataagg taggagatcc
    17221 caaactgaat cgcgaccatg gcaatcaagg ccggaaccag cccgaatccg aattctttca
    17281 tgaacatcca ggcaaggtat acggtcaggg taaaaaggta ggcgtaacct aaatgaaaaa
    17341 tccgcaacac cccgcagatg atggagaagc ccagtgagat gagcaggtaa atggacccga
    17401 gaacgaaggt gtagatgaga acgacttgga gggtttccca catattttgg tatccccctt
    17461 taaacccaaa gaagtgataa aaataaacca accctgctgc ctgctgaccg ggtggctcta
    17521 cagtccaccc cctcttataa taaactggga ttctttgtca agactttttt agttaagtaa
    17581 atagcgttaa tccatgatat gcatttaacc gaccaataaa tcaaaggaaa tttatgggga
    17641 aaaattaagg aggatgggta atctccggag gatgaattgc gggatgctgg gttctcgggg
    17701 attcaccccg tcttccctct caacatttct ttgtgtacct ggcgcaataa tttgaggagg
    17761 gcctgattct ctttgcgggt ccttacagag actcgaaaaa aataaggacc gaggtgatgg
    17821 aaggactggc agtcgcggat gaggattccc cgggggatca acttttcccg cagttctgcg
    17881 gcggtgaggt tcagggaagg atgaagctgg acgagaagat agttggccga gccgggatag
    17941 gggataaacc cgggaatccc ctgcaacccg agggtcagat tttcccgctc cgcctgattg
    18001 gaggccagcg ttcgctcaat gtaagtcttg tcccggaggc tctccacagc ggcgatctgg
    18061 gccagggcgt tgaccgccca ggggcccttt tcttcctcaa atttccggct gacctctgga
    18121 ttgcatacca tatacccggc ccgaagcccg ggcatggcaa agaaatgggt aagggatcgg
    18181 aggatgatga gccgggagga agacgccgct tcccggacaa gggattcccc cggaacaaaa
    18241 tcgatgaagg cttcgtccag gatgaaccaa accttctccc tttgcgcctg agccagaacc
    18301 cggaggaggt cctttttttc cgcgaggatc cccgtggggc tgttggggtt gcagagatag
    18361 agtgcatcgt acccctgggt cagtgccagc aggagcccct ctacgttgag ttcaaaaccg
    18421 tcttcttccc gggtttcaaa aaaaagaatc ttgggtcttc tctccccgct ttccagggcg
    18481 ttttcgtact cgctgaaggc cggggtcacg agtaggattc ggttcaattc catgatgcgg
    18541 gggatggaat aaatgaactc cgtggaaccg gccccgggaa ggattctgtc ctccggaatc
    18601 cggtgatatt cggcgagagc cttgcgaagt tcgcggcacc gggtatccgg gtaatgaaga
    18661 acggccgcaa gcctttcttt caagatcctc ttctgggcct taggggacag ccccaggggg
    18721 ttaatggagg cgctaaaatc gatgatcttt gccggatcga gatcttgctg cgcggcgact
    18781 tcgtaaatat tttctccgcg gactttgttc aagggaaatc cccaaaaaag acgttgagcg
    18841 gttgttgggg catgatgcat cgtgccccta cgggcctttg gggcctgggt tggtttcctg
    18901 agaggttcct gcaaaacttt cgagacatgg ggaatttgtc attcctggca accccggcga
    18961 aggccggggg cgacccggaa tccaggatat taagagaatt ttggattccg gctttcgccg
    19021 gaatggcggc gtaagcgact tttgcaggag gctttttaaa atctgcgttc atctgcgtcc
    19081 catttgtcct cagttgttcg aaacccgtgg cgccggaagg cggcggcaga gggtccaagc
    19141 ctggaagcgg ggcgtgtacc ggagcataaa ggtctctcct tctttcgtct tcacccggaa
    19201 ataatcgtgt cgcaacgctt tcgggtccaa tcccccttca taccaccggt ccaggatttc
    19261 tagaacctcg aatcgcttcc ccagatgaac aaaagccctc aagctctctt cgcctttgta
    19321 cccggaatag gattcaactt cgatgtcttc aaaaacccat tccataaggc agctttaagc
    19381 aatcagctat cagctttcag cgaaagaatc tgtgtttttg tattggctga aagctgatcg
    19441 ctgaccgctg tttttgggct gatcgctgac cgctgaaagc tgatggctat agtattttaa
    19501 agacgatgac caccttcagc catccttctt tgtagggaaa gggggcggcg cgctggaccg
    19561 tctcg
//
LOCUS       DASPRR010000251        12193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_62290, whole genome shotgun sequence.
ACCESSION   DASPRR010000251 DASPRR010000000
VERSION     DASPRR010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12193)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62290"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VEL68_09810"
     CDS             <1..505
                     /locus_tag="VEL68_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXZ36301.1"
                     /translation="AREKNLPVVQMDLDELGRRGETFDAVCGFQVLEHVHHARELLSA
                     MVQVLEPGGLLVLSVPNRDSFLRHQYNLLDMPPHHMTRWNGEALAYLSRIFPLRLLGL
                     RYEHLAPYHISGFVAAYGEYFGAKVPFLRRRLTKERLVRLADFIQQRGLNRFLRGQSL
                     YGVFQRL"
     gene            502..1311
                     /locus_tag="VEL68_09815"
     CDS             502..1311
                     /locus_tag="VEL68_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXZ36302.1"
                     /translation="MRLLNLGCGRRYHPDWVNVDFRSTGPGVLAIDLGGRLPFSDREF
                     DAVYHSHLLEHLPKRKVPFFLRECFRVLKPGGILRVVVPDLEKISRLYLDYLERAISG
                     DEEAQKRYEWILLEMFDQMVRNQPGGEMLNYWKQNPMPAESFVIERVGSEVLDLLPQL
                     RQSSAQGPPFNAFPSPLRGEGQGGGESAGWDPATDPMKIGEFRLSGEVHQWMYDRYSL
                     GRLLQAAGFQRGRVCRADESEVPNFKHYLLDIEANGAVRKPDSFFMEARKP"
     gene            1308..3020
                     /locus_tag="VEL68_09820"
     CDS             1308..3020
                     /locus_tag="VEL68_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ36303.1"
                     /translation="MKILQVSTYDLQGGAAIAAYRLHRGLRAMGEDCRMLVRHKSSAD
                     ETVLRVLSQDQPVPEDEDFFLRTVIQSQHIDSHRTEISNTLFTLPYPGDDLAGSTLFR
                     EADLVNLHWVAGYQSPITVQKLWQQGEPVVWTLHDQWAFTGGCHYSAGCLKYRSDCLS
                     CPQLADDPWDVPAAVLRDKLTLWARANLTIVTPSRWLAACARESALFRELRIEVIPNS
                     LDTEFFTPFPKEDAKRDLGLPPDAATLLFGAEEGSEKRKGFRELLAAMHHALAMPEFR
                     SLLERERLKILCFGHPDPTLRDEGLPVVALGYLESPERIRTAYSAADQFILPSLEDNL
                     PNTMLEALSCGTPVVAFAAGGIPEVVFDGVNGRLAPVGDSQELARSISSLIFRPEESK
                     SMGRNGRRLMVEEYSPPIQARRYKRLYQDLCEGHEPRKAPIPGKEAVDIPPTEVAGSA
                     DILRVPLETALGPHLQAILNPVLFRALKRYAPSLYRQWLDAEADRAARLEVIHRFGQR
                     IDELNRQVNDLNGPMGEWQRNFLRILRKLHLLPSPRWKGAPAQTVGNSEAPLNPESPK
                     KRAT"
     gene            3227..4189
                     /locus_tag="VEL68_09825"
     CDS             3227..4189
                     /locus_tag="VEL68_09825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ36304.1"
                     /translation="MLNHHKENPLFDLYMESELGSLKRSRVFMESLCHNLGNPGLFHG
                     KECLDVGSSAGNSLIAFIEGGARRAAGIEISTDRYETGMINIQECRPDVKAKIQMIRA
                     DIQDEGVASLGQFDIIFCTDVLEHVSDLQRAIANLCRLLKNAPEAFAYVKLRNYQHPE
                     NVLHEPHYDLPGMVLLPPERACKYFEFCQADRGLHYEVNEWKSFFEYQQLFQFFAKEC
                     SFGGPVNPDPSLTSEMERRGKALITEFDRFRETHSLPPDLEKEIRKYLNLYLKKMKRL
                     IQEYREKQSKELLERFYLEYGIYEIIMLVTNKEKLVLPKASFGS"
     gene            4259..6814
                     /locus_tag="VEL68_09830"
     CDS             4259..6814
                     /locus_tag="VEL68_09830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ36305.1"
                     /translation="MKEGFPTFNILILNGDLPVFPGWGGIEFLHTTNLARMAEKVGLV
                     SLVHTSEQREKKQALIDAGVHLFLWENPNSVAVLGSADSAIKIPSLGKRVGKAIFKIL
                     RMRVHRPLDTLIQDLVFRNLSGPLHKAFGANHWQALVIVQSNCARWLDFLPAFPVTVL
                     VLHDVRALLYERKRGVVRSKKERWACSVESWLYRRFERHYCHKYDLIVTVSPADEAWV
                     RKNYGLHRVITIPIPVDGNYFSPFSSVREDKARILFSGMMNHPPNVDAACFFAQEVFP
                     QVKAEIPEAEFWIVGRDPAPSVQALQELPGVIVTGFVPDIRPYIAQAMVFVVPLRFGS
                     GMRQKILEAWAMEKCVISTSLGAEGLNYRDGENILIADGAGVLAQKTVQALRSPSLRH
                     NVRSQGRQLVATDHHPEKLAQRYYQAIASALKAKKDKNRNAAMKAVIDLRWMRPGVAG
                     GIENLSRSFLRNLMPLDQTNQYTLLVPSEVKYDFDNRRNPNFRIRVMDSPIHLWRRGL
                     GELSHRLNQRLRIGALSTPELKSLLQARSFGAEIALSIPGYIAPDLHPFLNVLIVPDI
                     QHEYHPEFFSHAVLAERKKVYTRAIQRADHICAISEFTRQTLIQKLEVSPGKISVTHL
                     AADPIYHRGNNPPQQQDNILKKYSLPKGKYLFLPGHTWPHKNHPSAVKALRLLRESYH
                     LDLMLVCTGKPKESHPDLMKLICGLNLDSHVRFLGYCPMTDMPFLYGGAAALVFPSLF
                     EGFGIPLIEAMWCGCPVVCSNLTSLPEIAEDAGLTVDPHSPEEIASSVHRLLTDDNLR
                     QTLIARGHRQAKKFSWEKFTLSVVKIMYRTRESYYGRIVGDASNGKNAENHCC"
     gene            6783..8156
                     /locus_tag="VEL68_09835"
     CDS             6783..8156
                     /locus_tag="VEL68_09835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ36306.1"
                     /translation="MGKMPKITVVSPSFNQGRFIAETIESLMDQDYPCFEHIVIDGGS
                     TDQTLSILRRYPHLIVVSEPDRGQAEAINKGFRLATGEIWGFLNSDDTLLPGALKRVA
                     QEIDPEQERHIVMGRCRFVDENGDFTGIEHPSHFESHRRILAIWKGYTLPQPAVFWTP
                     EVWKVCGPMDEGLKFSLDYDLFCRFSREYPFHFVDQVLATYRLHPESKTEQWTERQRL
                     EDAIRISRRYWGSPWALKYWLLTFSLGWYRFNRTGRALAFYRQGQEHWRQHRHLQGAI
                     YASLGGMLAPDVAFYAGVYPVIRNSAARILLKAFEHFQRNRRRYPQTLAYLDHTDPWS
                     DGWAGPRLVVMREAHQTAQAVSIQGFADLTFLSAPLTLTLFVNGQRVGEKRFDKSGDF
                     LLEFPLPHPLESGRHAVEVQASSWFVRHSFARNRDFRPLSFRMGEIHFHSAVGEKKET
                     TVQWKEK"
     gene            8327..9295
                     /locus_tag="VEL68_09840"
     CDS             8327..9295
                     /locus_tag="VEL68_09840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ36307.1"
                     /translation="MSNPLVSIVTPSYNQGGFIARTIESVLSQDYSPIEYMVMDGGST
                     DETLEVLRRLGERFSWVSERDRGQADAINKGWRRSHGEILAWLNSDDFYLPGAISKAV
                     AFLQDHPEAAAVYGEGFHVDAKERIIERYPTEPFDLQRLYEFCFICQPTVFIRRVVLE
                     EVGFLDERLNFCMDYDFWFRIAKKHTFRYMKEPLACTRFYESTKTIEKRIDAHKEILA
                     VVFRHFGSVPPSWAYGYAYARIRPYFDRPPNFRNPFFVGGIAFLICSVFLRYNHRALL
                     AEGKRWGNWFCRNFHWPFSQPPQGPMGVYGLRRSLSKSHFRFGEKE"
     gene            9307..10329
                     /gene="gmd"
                     /locus_tag="VEL68_09845"
     CDS             9307..10329
                     /gene="gmd"
                     /locus_tag="VEL68_09845"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXZ36308.1"
                     /translation="MKKAMITGITGQDGAYLSKLLIEKGYEVYGAFRRTSDLHLNRLK
                     YLGVDEKIHYLPLELLEFTNLYKAIDKYQPDEVYNLGAQSFVALSFEEPIFTLDVTGL
                     GPLRILEAIRAINPRIKFYQASSSEMFGKVRAVPQEENTPFHPRSPYAVAKLSAHWIT
                     VNYREAYNLFACSGILFNHESPLRGLEFVTRKITYGLARIQKGLQETIVLGNLNARRD
                     WGYAPEFVEAIWLMLQQPQPEDFVIATGETHTIREFVEEAFRVVDVEIAWQGSEEKER
                     GLDRRSGKVRVEVSPEFYRPAEVDLLLGNPRKAGEKLGWRPRTSFKNLVRIMVEADLE
                     RVRKEE"
     gene            10326..11456
                     /locus_tag="VEL68_09850"
     CDS             10326..11456
                     /locus_tag="VEL68_09850"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXZ36309.1"
                     /translation="MKIAINTIPLLFPLTGVGNYIYHVARSLQKTEAGHEYSYFYGYY
                     SRTLIPPGRNPKGVHRVKEFVQGLPLLGAMARDLQDLRGFLSRRKFDLYFEPHFIPLH
                     IPARRTVVTVPDFSFARFPEWHTRDKVRYFQKNFWKKISRADRVIFISRFIQQEAMDL
                     FGFALERSSVIHLGYDREVFRPYPVRELIPVQKQYNLPEKFILFVGSIEPRKNLKNVL
                     RAYLNLEKGLVGEYKLVLVGFKGWENKEVMGMIRMRGSDVRYLGYVPESDLGKIYSLA
                     RLFIYPSFYEGFGLPPLEAMACGCPVIASQAASLPEVCGDAACYVDPLEASSIREGME
                     RVLTEESLRTALISKGLKRAAEFSWEKCARAHLAVFEELYGS"
     gene            complement(11560..>12193)
                     /locus_tag="VEL68_09855"
     CDS             complement(11560..>12193)
                     /locus_tag="VEL68_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXZ36310.1"
                     /translation="RGAWVPGALILLLAGWKFTSRNVFLPILLAAGVCVLMLSPPNLF
                     WHTTPVRGPGAPAGKIETANVRWEAVKFSLNQVAEHPFRMIGFGRRSFVKEFQDFYLK
                     YEGSLIWHAHNTFLNLALQTGIQGLAIFCLLLYGLIKYFYVGAKQEEPVQLLFFNQAT
                     LWMIIAFFMRNLSDDFFIDDSALLFWFLVGAGMVINKSPKVHAAADAIAQ"
BASE COUNT         2828 a         3257 c         3232 g         2876 t
ORIGIN      
        1 ggcccgggag aaaaacttgc ccgtggtgca gatggacctc gatgaactgg gccgccgggg
       61 cgaaaccttt gatgccgtct gcggttttca ggtgctggag catgtccatc atgctcggga
      121 actcctgtcc gccatggtcc aagttctgga gcctggaggc ttgctcgtct taagcgtgcc
      181 caaccgggat agttttctcc ggcaccagta caaccttctg gacatgccgc cccatcatat
      241 gacccgttgg aacggagagg ctctggcgta tttgtcccgt atatttccat tacgacttct
      301 gggccttcgt tatgaacatc tagctccata ccacatctcg gggtttgtgg cggcctacgg
      361 agaatacttt ggcgccaaag tcccattcct ccgacggcgt cttaccaagg agcggttggt
      421 ccggctggct gatttcatcc agcagagagg gctgaaccgg ttcctccggg gccaaagcct
      481 gtacggggta ttccaaaggc tatgagactt ctgaatctgg gttgcggtcg ccgctatcat
      541 ccggactggg tcaacgtgga ctttcgttcc acggggccgg gggtcttggc tattgacctg
      601 ggcggaagac tccccttcag cgaccgcgaa ttcgacgctg tctatcattc ccacctgctg
      661 gaacacctcc ccaagcggaa agttcccttt tttcttcggg aatgcttccg agtcttgaag
      721 ccggggggaa ttttgagggt cgtggtcccg gacctggaaa agatttcccg cctctatctg
      781 gactacctgg aaagagccat tagcggcgac gaggaagccc aaaagagata cgagtggatt
      841 ttactcgaga tgttcgacca gatggtccgc aatcagcccg gaggggagat gctgaattac
      901 tggaagcaga accctatgcc tgctgagtct tttgtcatcg agcgggtggg ttcggaagtg
      961 ctggaccttc tccctcagtt gcggcaatcg tccgcccagg ggcccccctt caatgctttt
     1021 ccctccccct tgagggggga gggccagggt gggggtgaat cggcagggtg ggatccggcc
     1081 actgacccaa tgaaaatagg agaatttcgc ctctccggag aagttcacca gtggatgtat
     1141 gaccgttatt ctttgggaag gctattgcag gcggcgggat ttcagcgcgg gagagtctgc
     1201 cgggccgacg aatcggaggt cccgaatttc aagcattatc ttctggacat tgaagccaac
     1261 ggagcggtgc gaaagccaga ctccttcttt atggaggcca ggaagccttg aagattttgc
     1321 aggtgagcac ttatgacctt caaggggggg ctgccattgc agcctatcgt ctgcatcgcg
     1381 gtttacgggc aatgggggag gactgccgga tgctggtccg gcataagtcc tcagccgatg
     1441 aaacggtttt acgggttctc tcccaagacc agccggtacc cgaggatgaa gatttctttc
     1501 tccgcacagt gattcaaagc cagcacattg attctcaccg gacagaaatc agcaataccc
     1561 tgtttaccct tccctatcct ggagacgacc ttgccggttc aaccctgttc cgggaagcgg
     1621 acctggtcaa tctgcattgg gtggccgggt accagagccc gatcaccgtg caaaagcttt
     1681 ggcagcaagg cgagccagtg gtgtggactt tacacgatca atgggctttc accggaggtt
     1741 gccactattc cgcgggttgt ctcaagtacc ggagcgattg cctctcttgc cctcagctgg
     1801 ccgatgatcc ctgggatgtt cccgctgccg tcctgcggga taagctgacg ctatgggccc
     1861 gggccaatct gacgattgtc actcccagcc gctggctcgc tgcctgcgca cgggaaagcg
     1921 cgctcttcag ggaactgaga attgaagtca tcccgaattc cctggacacg gaattcttca
     1981 ccccctttcc caaagaggat gccaagagag acctgggcct tcccccggat gcggcgaccc
     2041 ttctctttgg cgccgaggag ggaagtgaga aacgaaaagg atttcgggaa ctgctcgcag
     2101 ccatgcacca tgccctggcc atgcctgaat ttcggagcct tctggaaagg gagcgcctga
     2161 aaattctctg cttcggccat ccggacccca ctttgcgtga cgagggcctg ccggttgtgg
     2221 ccttaggata cctcgaatca cccgaaagaa taagaaccgc ctattccgcc gcagaccagt
     2281 tcattctccc ttccctggag gataatctgc ccaataccat gctagaggcg ctcagttgcg
     2341 gaacgccggt cgtagctttt gctgcaggag gaatcccgga agtggtcttc gacggggtga
     2401 atggaaggct ggccccggtg ggcgattcac aggaattggc ccgatccatc tcttccctga
     2461 ttttccggcc ggaggaatcc aagagcatgg gccgaaacgg ccggaggttg atggtggaag
     2521 aatactctcc gcccatccag gcccggcgct ataagcgctt atatcaggac ctctgcgagg
     2581 ggcatgaacc ccggaaagcc cccatccccg gaaaggaggc cgtagatatc ccaccgacag
     2641 aggttgccgg ttccgccgat attctgagag tccctctcga aacggccctg gggcctcacc
     2701 tgcaagcgat tctcaacccg gttctcttcc gggccctcaa acggtacgcc cccagcctgt
     2761 accggcaatg gctggacgcc gaagccgacc gcgcagcccg tctggaagtg attcatcgtt
     2821 ttgggcagag aatagatgaa ctcaaccgac aagtaaatga cctcaacggg ccgatgggtg
     2881 aatggcagag aaacttcctt cggattctca gaaaattgca ccttttgccc tcccctaggt
     2941 ggaaaggtgc tccggcccaa acagttggga attcggaagc accgctgaat ccggaaagtc
     3001 cgaagaagcg agccacttga tcattgactt tatgaacctg caaagaataa aaaaaatatt
     3061 caagggtata cccatcgctt tttttcggag agaatcaaca ggtgaaaaag ggggaagcgg
     3121 aaatctggaa cccatcccca gggattggaa agaagaaaaa ctccttgcct atctgcttca
     3181 tcggtacgga accaatcgga agtatacgga attctatcta aaacatatgt tgaaccacca
     3241 caaagaaaat cccctctttg atctctacat ggaatcggag ctcgggagcc tgaagcgaag
     3301 ccgggttttc atggagtccc tatgtcataa tttggggaac cctggattat tccacggcaa
     3361 agaatgcctc gatgtggggt cttccgcggg gaattcgctc atcgctttca ttgagggggg
     3421 tgcccgacga gccgccggga tcgaaataag tacggacagg tacgaaacag gcatgatcaa
     3481 catccaggaa tgtcgccccg acgtcaaggc gaaaatccaa atgattcgcg cagacattca
     3541 ggacgagggg gtggcctccc tgggccaatt tgatatcatt ttttgtaccg acgtcctgga
     3601 acatgtgagc gatctccaaa gggcaatcgc gaatttatgc cgtttgctca aaaacgcacc
     3661 cgaggccttc gcttacgtga aactgagaaa ttatcaacac cccgaaaatg tcctccatga
     3721 accgcattat gatctgccgg gcatggtctt gctccctccc gagcgggcgt gcaagtattt
     3781 cgaattttgt caggctgacc gcgggctcca ttacgaagtc aatgaatgga agtctttttt
     3841 tgaataccag caattatttc aattttttgc caaggaatgt tcgtttgggg gccccgtgaa
     3901 ccccgaccct tctttgacct cagagatgga aagacggggg aaggccttaa taactgaatt
     3961 cgatcggttc cgggagacgc attctttgcc cccggacctg gaaaaggaaa tccgaaagta
     4021 cctcaacctc tacctcaaaa aaatgaagcg cttgattcaa gaatatagag aaaagcagag
     4081 taaggaattg ttggaaaggt tttatctcga gtacggcatc tatgagatta ttatgcttgt
     4141 taccaacaag gaaaaactcg ttcttccaaa agcgagcttt gggtcctgac tttttcgcca
     4201 gggcccccct ccgagaacgg gttatttccc aattttcttg gaccaaaggc tcatcgagat
     4261 gaaagaaggt ttcccgacct tcaatatttt gattctaaat ggagacctgc cggttttccc
     4321 tggctggggc ggaattgaat ttttacacac cacgaatctg gcccgcatgg ccgaaaaagt
     4381 gggcctcgtt tccttagtcc atacgtcgga acaaagggag aagaaacagg ctttgatcga
     4441 tgcgggagtt cacctctttc tttgggaaaa tccaaactcc gtcgctgtcc tcgggtctgc
     4501 cgactccgca ataaaaattc cgtctctggg aaagcgagtc ggaaaagcca tcttcaaaat
     4561 attgcggatg agggtccatc gtcccttgga taccctgatt caggaccttg ttttccgcaa
     4621 cctctcgggc ccccttcaca aggccttcgg cgctaaccac tggcaggctt tggttatcgt
     4681 ccaaagcaat tgtgcccggt ggcttgactt ccttcccgcc ttccctgtta ctgtccttgt
     4741 cctgcatgac gtccgggccc ttttatatga aaggaagaga ggcgtcgtcc gatcaaaaaa
     4801 ggagcgctgg gcgtgttctg tggaatcctg gctttaccgg cggtttgagc gccattattg
     4861 ccacaaatat gatctgatcg tgaccgtatc ccctgcggat gaagcgtggg tccgcaagaa
     4921 ttatggcctc catcgagtca tcaccatccc cattcctgtt gatgggaatt acttttctcc
     4981 cttttcgtcc gtgcgcgaag ataaggctcg cattcttttc tcggggatga tgaatcatcc
     5041 tcccaacgtg gacgccgcct gcttttttgc ccaagaggtc ttcccccaag tgaaggccga
     5101 aattcccgag gcggagttct ggattgtcgg ccgggatccc gctccaagcg ttcaggctct
     5161 tcaagaactg cccggggtca tcgttacggg ttttgttccg gatattcgcc cctacatcgc
     5221 ccaggcaatg gttttcgttg tgcctttacg ctttggctcc ggaatgcggc aaaaaatact
     5281 ggaagcgtgg gcgatggaaa aatgcgtgat ttcaactagc ttgggcgcag aagggttaaa
     5341 ttatcgagat ggagagaata ttctcatcgc ggatggggcc ggtgtcttag ctcagaaaac
     5401 agtccaggcc cttcgcagcc cttcccttcg ccataacgtt cgctctcagg gaagacagtt
     5461 ggttgccacg gaccaccacc cggaaaaact ggctcaaaga tattatcaag ccattgcctc
     5521 cgccctgaag gccaaaaagg ataaaaatcg gaatgcggcc atgaaggcgg tgatcgacct
     5581 gcgctggatg cgccctggag ttgcgggggg aattgagaat ctctcccgtt ccttcttgag
     5641 aaatttgatg ccgctggatc aaaccaatca atacaccctt ctcgttccgt ccgaggtgaa
     5701 atatgatttt gataatcggc gcaatcctaa tttccgaatc cgggtaatgg atagtcccat
     5761 acacctttgg cgtcgggggt tgggggaact atctcatcga ctcaatcagc gactgagaat
     5821 tggagctctc agcactcccg aattgaaatc cctgctgcag gctcgttcct tcggggcgga
     5881 aattgctctg tcgatccccg gttatattgc cccggatttg catcctttct tgaatgtcct
     5941 gatcgttccc gacatccagc atgaatacca tcccgaattc ttttcccatg cggtcctggc
     6001 cgaacgtaaa aaggtatata cccgcgccat tcagcgggcc gatcacattt gcgctatttc
     6061 cgaatttacc cgacagacgc tcattcaaaa attagaggtt tcccccggca aaattagcgt
     6121 tactcaccta gccgctgatc ccatctacca tcgaggcaac aatccgcctc agcaacaaga
     6181 taatatttta aaaaaatata gcctgccgaa aggaaaatat ctatttttgc ccggccacac
     6241 ttggccccat aagaaccacc cttcggccgt gaaagctttg cggctcctcc gggaatctta
     6301 ccatttggac cttatgctgg tctgcaccgg caaaccgaaa gaatcgcatc ctgacctcat
     6361 gaaattgatc tgcggcttga acttggattc ccacgtacgc tttttggggt actgtcccat
     6421 gaccgacatg cctttccttt acgggggggc ggcagccctg gtttttccgt cgcttttcga
     6481 aggatttggg attcctttga ttgaagcaat gtggtgtggt tgtcccgtcg tttgcagcaa
     6541 cttgaccagc ctccccgaaa ttgccgagga cgccggcctg acggtagacc cccattcccc
     6601 agaagaaatt gcttcgtctg tccatcggct tctcaccgac gacaatttgc gccagaccct
     6661 gatcgcccgc ggccaccggc aggcgaaaaa attctcctgg gaaaagttta ccctatcagt
     6721 ggtcaagatc atgtatagga cccgggaatc ttattatgga cgaattgtcg gggacgcttc
     6781 gaatgggaaa aatgccgaaa atcactgttg ttagcccttc ctttaatcag ggaaggttca
     6841 ttgccgaaac catcgaaagc ctgatggatc aggattatcc ttgctttgag catatcgtca
     6901 tcgatggagg gtctacggat cagactcttt ccattttaag gcgctatcct catttgatcg
     6961 tggtttctga acctgaccgc ggccaagctg aagctattaa caaaggtttc cgcttggcca
     7021 ccggcgaaat ttggggtttt ttgaattccg atgatacgct tctgccgggg gccttaaaac
     7081 gagttgccca ggaaattgac ccggagcagg agcggcacat tgtcatgggt cgttgtcgct
     7141 ttgtcgacga aaatggggat ttcaccggga ttgaacatcc aagccatttc gaaagtcatc
     7201 gccggatatt ggccatctgg aaaggatata cccttcctca gccggcagtc ttttggacgc
     7261 ccgaagtgtg gaaagtctgt ggtccaatgg atgagggatt gaagttttcc ctggactacg
     7321 atttattctg ccgattttcc cgggaatatc cttttcactt cgtcgaccag gtcctcgcca
     7381 cctatcgtct tcatccggaa tccaaaacag agcaatggac ggaacggcaa agactggaag
     7441 atgcgattcg cataagccga aggtattggg gatccccgtg ggcgctgaaa tattggcttc
     7501 tgaccttttc tctgggctgg taccgcttca accgcaccgg gcgagccttg gcattctatc
     7561 gccaagggca agagcattgg cggcaacatc ggcacctgca gggcgcgatt tatgcctccc
     7621 tgggagggat gctggcaccc gatgtcgcct tctatgccgg ggtttatcca gtaatacgca
     7681 actctgccgc ccgcatcctg ctcaaagcct tcgagcactt tcaaaggaat cgtaggaggt
     7741 atcctcaaac gctggcttac ttggatcaca cagacccatg gtccgatggc tgggccggtc
     7801 cccgcctggt ggtcatgcgg gaagcccacc aaactgcgca agcggtttcg attcagggtt
     7861 tcgccgattt aacctttttg tctgcacctc ttaccttaac cctttttgtc aatggccaga
     7921 gggtgggaga aaagcgtttc gacaaaagcg gagattttct gttggagttt cctttgccac
     7981 accccctaga atcaggccgg catgcggtcg aagtccaggc cagttcctgg ttcgtgcgcc
     8041 acagctttgc ccgcaaccgg gactttcggc ccctgtcttt tcgaatgggc gaaattcatt
     8101 ttcattccgc tgtcggcgag aaaaaagaaa ccaccgtcca atggaaagaa aaatagaacc
     8161 ttctttccac aaagtggaag gactccctag gggccaggat ccggatggct catagcatag
     8221 gccgggcaat gaccgcggaa aactggttat ccgcgaatgg gcgcgttttc gattcttaag
     8281 attcatggca cgggtgttca tccgggaagg aaatcaggag tcatcgttga gcaatccact
     8341 ggtgagcatt gtaactcctt cttataatca aggcggcttc atcgcccgga ccatcgaaag
     8401 cgtcctatcc caggattatt ccccgatcga atacatggtg atggatggag gatcaaccga
     8461 tgagaccttg gaggtcctta ggcgattggg cgagcgcttt tcttgggtct ccgaaaggga
     8521 ccgaggccaa gcggacgcca tcaacaaggg ctggcggcgc tcccatggtg aaatccttgc
     8581 ctggttgaac tcagacgatt tttatctccc gggggccatc tccaaggcag tggcttttct
     8641 ccaagatcat cccgaagcag ccgcggtcta cggcgaggga tttcatgttg acgcaaagga
     8701 gcggatcatt gaacgatacc ccaccgaacc ttttgacctg cagcggttgt atgaattctg
     8761 ttttatctgc cagccaacgg tgtttattcg gcgagtggtt ttggaagagg tgggattcct
     8821 ggacgagagg ctgaacttct gcatggacta tgacttctgg tttcggattg ccaagaaaca
     8881 tacctttcgc tatatgaaag aacctttagc ctgcacccgg ttctatgaat cgacgaaaac
     8941 catcgaaaag agaatcgatg ctcataaaga aattctggct gttgttttcc gccacttcgg
     9001 gtccgttcct ccttcatggg cttacgggta tgcctacgcc aggataagac cttatttcga
     9061 ccgtccgcca aatttccgga accctttttt tgttggcggg attgcttttc tgatctgttc
     9121 agtcttcctc aggtacaacc atcgagcttt gcttgcggaa ggcaaacggt ggggaaattg
     9181 gttttgcagg aatttccatt ggcctttttc ccagcctcca caaggtccaa tgggagtcta
     9241 tgggttaaga aggagtctct ccaaaagtca ttttcgattc ggtgaaaagg aatgaaaggt
     9301 gcaaaaatga aaaaagcaat gatcaccggg attaccggtc aagatggagc ctacctctca
     9361 aaattactca tagagaaagg ctatgaagtc tacggggctt tccgaaggac gtcggacctt
     9421 cacttgaacc ggctgaaata cctgggagtg gatgagaaaa tccactatct cccgttggaa
     9481 cttctggagt ttaccaatct ttacaaagcc atcgacaaat atcaacccga tgaggtctat
     9541 aacctgggag cacagagctt cgtagccctt tcctttgaag agcccatctt cactctggat
     9601 gtcaccggtc tgggcccctt acggattctg gaagcgatcc gggccatcaa tccaaggatc
     9661 aaattctacc aggcctcctc cagcgagatg ttcgggaagg tgcgggctgt cccacaggaa
     9721 gagaacacgc ctttccatcc tcggagccct tacgcggtgg ccaagctctc cgcacattgg
     9781 ataactgtga actaccggga ggcgtacaac ctgttcgcct gttccggcat cctgttcaac
     9841 cacgagtccc ccctcagggg cctggaattc gttacccgca aaatcactta tgggctggct
     9901 cgaatccaga aggggctgca ggagaccatc gtcctgggca acctgaacgc ccggagggac
     9961 tgggggtacg caccggaatt cgtggaggcc atctggctca tgctgcagca gccgcagccc
    10021 gaagacttcg tcatcgccac gggagagacc cataccatcc gtgagttcgt ggaggaggct
    10081 ttccgggtgg tggacgtgga aattgcctgg cagggttccg aggagaagga aaggggattg
    10141 gaccgcagga gcgggaaggt ccgggtggag gtatccccgg aattctacag gcctgcggaa
    10201 gttgatcttc ttttggggaa cccccggaag gcgggggaga agcttggctg gaggccccgg
    10261 acatcattca agaacctggt ccggattatg gtggaggcgg atttggagag ggtccgcaaa
    10321 gaggaatgaa aatcgccatc aacacgattc ccttgctctt ccccctgacc ggtgtaggga
    10381 attacattta ccatgtcgcc cgatcccttc agaaaaccga agcgggccac gaatatagtt
    10441 atttctacgg gtactactcc cggaccctca tccctcccgg gcggaatccc aagggggttc
    10501 atcgggtcaa ggaattcgtc caggggcttc cccttctcgg ggccatggcc cgggacctcc
    10561 aagacctgcg agggttcctt tcccgccgga aattcgatct ttatttcgaa ccccacttca
    10621 ttcctctaca tatccccgcc cggcggactg tcgtcaccgt gccggatttt tcctttgccc
    10681 gctttcccga gtggcatacc cgggataagg ttcgctactt ccagaaaaac ttttggaaaa
    10741 aaatcagccg ggcagaccga gttattttca tctctcggtt catccagcag gaggccatgg
    10801 acctatttgg cttcgccctc gaacggtcgt cggtcatcca tctgggctac gaccgggagg
    10861 tcttcaggcc ctaccctgta cgggaactga ttcccgtaca aaaacaatat aatctacccg
    10921 aaaaattcat cctctttgtg gggtccatcg aaccccgaaa aaacctgaaa aacgtactaa
    10981 gggcttacct taatctggag aaggggctgg tgggggaata caaactcgtc ctggtgggat
    11041 tcaaagggtg ggaaaacaaa gaagtcatgg gtatgatccg aatgaggggt tcggatgttc
    11101 gataccttgg atatgtgccc gagtccgacc tgggcaagat ctacagcctt gccaggctct
    11161 ttatctaccc ttctttctat gaagggttcg gccttcctcc cttagaggcg atggcctgcg
    11221 gatgtccggt gatcgcctcc caagccgcta gcctgccgga agtctgcgga gacgcggcat
    11281 gctatgtgga ccctttggag gcctcgagca ttcgggaagg aatggaaagg gtcctcactg
    11341 aagaatcgtt acgaaccgcg cttatctcca aggggcttaa gagggctgcg gagttcagct
    11401 gggagaaatg cgctagagcc catctcgctg tttttgaaga gctctacggg tcctaacctt
    11461 ttcccttcac cctccgcccc tcgaataagg ggcaacacct tcagggtggc gccattggtc
    11521 tttaccgaaa ccgatgattt cccccggggg aacatcaggt cattgggcga tagcatccgc
    11581 tgctgcatgg actttgggac tcttattgat caccataccc gcccccacaa gaaaccagaa
    11641 aagcaaagct gaatcgtcga taaaaaaatc atccgaaagg ttgcgcataa aaaatgcgat
    11701 aatcatccac aatgtggcct ggttgaaaaa aagaagctga acgggctcct cctgttttgc
    11761 ccccacgtaa aaatacttaa tcaacccgta caatagaagg cagaaaattg ccagcccttg
    11821 aataccggtc tggagggcca aattcaagaa ggtgttatgg gcatgccaga ttaatgaacc
    11881 ctcatatttt aggtagaaat cttgaaattc ctttacgaaa ctcctccttc cgaaccctat
    11941 catccgaaaa gggtgttcag cgacctggtt tagggagaat ttaactgctt cccagcgcac
    12001 atttgcggtc tcaatcttcc ctgccggggc tccggggcca cggaccgggg tagtgtgcca
    12061 gaaaaggttt ggaggtgaca acataagaac gcagactccc gcagccagaa gaattggcaa
    12121 gaagacattg cgggaggtaa atttccaccc tgcaagaagg aggattaacg cccctgggac
    12181 ccaggccccc cgg
//
LOCUS       DASPRR010000252         6343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1505890, whole genome shotgun sequence.
ACCESSION   DASPRR010000252 DASPRR010000000
VERSION     DASPRR010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505890"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..3379
                     /locus_tag="VEL68_09860"
     CDS             176..3379
                     /locus_tag="VEL68_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXZ36311.1"
                     /translation="MAKYNQAREPKGKPTLFLGPFQPELEEAFFLWVREKKEKDPFAP
                     LVVLVGSNLLGLYLRRLLTQRGLNHINLRFLTFIDLARTLAAEPLDRKGLRPLPRYGD
                     LVLISALAEKIQEGSYFGPIAGRRGFQRALAATFRDLADGGVETLPLLETRKISDLNP
                     LYQNYRSLAGKGFYSSSELFSHGEKEAQRFPQIFGSEELAVYGFYDFTESQKRLLEAC
                     IRHLRLTSFMPWRETQGFAYAAPVLQWYRSREFQVQSVARPELAGSKLVEVAPREIFA
                     ERPKAFSTREDRTVRIIAAPSEAQEVREIAREVLRLAREEEIPFHEMAVLLRNFDLYA
                     PLLQETFQNLQVPIHLHGGKPLSRTQAGKSVLLLLDLVGGNLRRGEVIEFLTFAPLAW
                     ERFFPEEPSTSHWDLISREAGIVEGREEWEERLTSWIAQQEQQEDEEGDQTLIQEAER
                     FHGFLKDFFPALDQFPRNSTWQGMVKACLLLLESYFEANGDREEIGDALRSLVSLDLL
                     GLEVEVRQFKEVLSEALEEKTLAHGAFQRGGVCVSDLMPARGLSFRAVFIPGLVERSF
                     PASPRQDPLLLDAERQAMNRDLKEKGHLPLKRYRFAEEKLLFTLALGAAREKVILSYP
                     RLDPSTGRERIPSFFLLRVGEVLCGEAMDYSRLERIPQYGCVPLSRLAPEDSAEAIDE
                     GEFDLSQVGKALRTHDRREVSYFRRAFPFFNRAEKLARLRWGFQAFTEYDGCMKSARA
                     RKLLRERFALSGQVLSATKLETYAACPFNYFLAEVLGLRPLPSPEEVRRIQPLERGTL
                     VHEILFRFYEASSKRYPGPLRSENLEGYWKALEEAASISFAAAEAEGWTGYPLLWEID
                     RQSILEDLRAFLEKETQAEEGMIPSLFEVRFGHGGLSPRKGGVDEPIPLMLEDGMGFF
                     LRGRMDRVDFSPERDALRVIDYKTGKLQGEEDGFCGGTTLQLPLYLMAACRTWKEADL
                     EKSWAEYCSVSRKGKFARIPFRGDGWEEKEANLKKILETIFQGISEGTFFPYREKSQG
                     CSFCDFRNLCEHGVDVLFEKKKKDPRAAAFLEIRQIP"
     gene            3617..>6343
                     /locus_tag="VEL68_09865"
     CDS             3617..>6343
                     /locus_tag="VEL68_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXZ36312.1"
                     /translation="MNSKLSLADEAAREIARNDLDRSLAVEAGAGTGKTTLLVERILS
                     LVRNRRAPLEKIVAITFTEKAAGELKVRLREAIEKALPLSPPAEAESLGQALGELERA
                     PISTIHSFCSSLLRERPVEASLDPNFEPLDEMGMDLLFQEAWEQWLGKEMEKKPAVLR
                     RAMALGMEMDHLARLVRQVYENRDLLPEGPLPQSSFSPESFIEILGKEVQRAWDLAQS
                     DCRKEEDLGYQAILGLRGKAKELKEASPDRQEVILFRDLEIKAQGNKSNWKPPPSCDA
                     QKEILNNLAKELGALKETLRAQVMAGLIGWVKGFVAAMQEEKSRRGVLDFQDLLILSR
                     NLLRDNKEVREYFQKNFQYILVDEFQDTDPLQVEVVFFLAEKGAKANRWEEVDLQPGK
                     LFLVGDPKQSIYRFRRADIETYEKARERVISRGAGVNIVQNFRTVPSILSWVNRIFSG
                     LIQSSMEGTYQPSYVPLVPHPGRKEVIKAQPGVLLLAPPPDFDPREASAAKVREDEAQ
                     SIAALIEEMAAGGPEGRWMVFDKKEVAPRPVSYRDMALLFPALTGIDAYEEALKDGEI
                     PYRLEGGKEFYMRQEVRSLLCCLKALDDPADRISLVAALRSPFFGFSDEEIFLFSASG
                     NRLSYLHHAEEETGEFSEALSLMRELHKRRNSRPISTTVSDLLSQTKALEFSLLRQGG
                     DQMAANLRKVLEQARAFEGEAQANFRRFVEWLGTREEEGVREGESPWAEEGEENVKLM
                     TIHKAKGLEFPIVFLANLASEGRRRQEFIPLRLQGTFEMRIGNFQTKGYSSALEQEKA
                     KMEAEDRRLFYVAATRARDYLVLPLFWGRRGRGFFSLLEENLPAFESMEPWSVIDGQL
                     IAGRGCFNLRPAEKPPLRLDLEESEKEKKEEGTPWERRLQWKESLDAVKEKAS"
BASE COUNT         1376 a         1773 c         1867 g         1327 t
ORIGIN      
        1 gtacattttt tttatttacc tcaagggagt tatccacagc cccttcgctc acctgctccc
       61 gggggcgcgt cgctccggga ctatggataa ctcccttggg taatatacaa ggggggatta
      121 tgcctttcca ttccgatcga aacccgttat cattcattat atcggaattc caaccatggc
      181 caaatataat caagcgagag aaccgaaggg aaagccgacc ctgtttctcg ggcctttcca
      241 gcccgagctg gaagaggctt ttttcctatg ggtcagagag aaaaaggaaa aggacccttt
      301 tgcccccctc gtggttctgg tcgggtccaa tttgctgggc ctttacctgc gaaggctgct
      361 aactcagcgg ggtctgaatc atatcaacct ccgtttcctc accttcatcg acctggcccg
      421 aactttggcc gctgaacctc tggatcggaa agggctccgg cctcttccgc gatacggcga
      481 tcttgtcctc atttctgccc tggccgaaaa aattcaggaa gggagttact tcggcccgat
      541 cgccggccgg aggggctttc agcgggctct ggcggccacc ttccgggacc tggccgatgg
      601 cggggtcgaa accctgcctt tgttggaaac ccgcaaaatc tctgatctca accccctgta
      661 ccagaattac cgttcgctgg caggcaaggg tttctactct tcttctgaac ttttctccca
      721 cggcgaaaag gaagcgcaac gtttccccca aatattcggc agcgaggagt tggcggttta
      781 cgggttctat gacttcaccg agagccagaa gcggcttctt gaggcctgca tccggcatct
      841 gcgactgact tcttttatgc cctggcggga aacccagggt ttcgcgtatg cagctccggt
      901 cctccaatgg taccggagtc gcgaattcca ggttcaatca gttgcaagac cagagcttgc
      961 tggaagcaag ctggtggaag tcgcgccaag ggagattttc gcggaaaggc ccaaagcttt
     1021 ttccacccgc gaggaccgca cggttcgaat cattgctgcc cccagcgaag ctcaggaggt
     1081 gcgggagatt gcgcgggagg tgctccgcct ggcccgggag gaagaaattc ccttccatga
     1141 aatggccgtc ctcctgcgca acttcgacct ctatgcccct ctcctccagg agacttttca
     1201 aaatctccaa gttcccatcc atctccacgg gggtaaacct ctctcccgga cccaggctgg
     1261 aaagagtgtg ctcctccttc ttgacttggt gggcggcaac ttacgacgag gagaggtcat
     1321 agagttcctg actttcgctc ccctggcctg ggagcgcttc tttccggaag aaccttccac
     1381 ttcccattgg gacctcatct cccgggaggc gggaattgtg gaggggagag aggagtggga
     1441 ggagagactg acctcctgga tcgcccagca agagcaacag gaagatgagg aaggggatca
     1501 aaccctgatc caggaggcgg aacgcttcca tggttttctg aaagatttct tcccggccct
     1561 ggaccagttt ccccggaatt ccacctggca gggaatggta aaggcctgcc ttctcctgct
     1621 ggaatcctat tttgaagcaa acggggaccg tgaagaaatc ggcgacgctc tccgatcact
     1681 ggtctctctg gaccttctcg gcctggaggt cgaggttcga caattcaagg aggtcctcag
     1741 cgaagccctg gaggagaaga cccttgccca cggggctttc cagagaggcg gagtgtgcgt
     1801 gtcggatttg atgcccgccc gcggtctatc cttccgggcg gtcttcatcc ccggcctggt
     1861 tgaacggtcc tttccggcat ccccccgcca ggaccccctg ctattggacg cggaacgtca
     1921 ggccatgaac cgagacctga aggagaaggg ccatctgccc ttgaagcgat accgcttcgc
     1981 cgaagagaaa ctcctcttca ctctggccct gggagccgcc agggagaagg tgatcctttc
     2041 ctacccccgt ctggatccct ccaccgggag ggaacggatt ccttcttttt tcctgctccg
     2101 ggtaggggaa gtcctttgcg gggaagccat ggattacagc cgtctggaga ggattccaca
     2161 atacgggtgc gtgcctctct cccgactagc ccccgaggat tcagccgaag ccatcgatga
     2221 aggggaattc gacctgagcc aggtaggcaa agcgctgcgc acccatgatc gaagggaagt
     2281 ctcctatttt cggagggcct ttcctttctt taaccgggcg gaaaagctgg cccgcctgag
     2341 gtggggcttc caagccttca ctgagtacga cggatgcatg aaatctgccc gggcccgcaa
     2401 gcttttgcgc gaaaggttcg ccctctctgg acaggttctc tccgccacca aactggaaac
     2461 ctatgcggca tgtccgttca attactttct ggcggaggtt ttaggtctca gaccgcttcc
     2521 ctctcccgag gaggtccggc gtatccagcc cctggagcgg gggactctgg tgcacgaaat
     2581 tcttttccgt ttctacgagg cctcctcaaa gagatatccg ggtcctttgc ggtcggaaaa
     2641 cctcgaagga tactggaagg cccttgagga ggcagcatcg atctcttttg ccgccgcgga
     2701 agcggagggc tggaccggtt atccgctgct ctgggagata gaccgccagt cgatcctcga
     2761 ggacctgcgg gccttcctgg aaaaggagac gcaagcagaa gaagggatga ttccttccct
     2821 tttcgaggtt cgtttcggac acggtggcct gagtccccgg aaaggcggcg tggacgaacc
     2881 cattcccttg atgttagagg acggaatggg ctttttcctc aggggtcgaa tggaccgggt
     2941 ggacttttcc cccgagagag acgccctgcg ggtcattgat tacaagaccg gaaaattgca
     3001 gggcgaggag gacgggttct gcggcgggac caccctccag ctccccctct acctcatggc
     3061 ggcctgccgg acctggaagg aagccgactt ggaaaagagc tgggccgagt attgcagcgt
     3121 aagccggaag ggaaagtttg cccgcattcc tttccgcggg gacggctggg aggaaaagga
     3181 agcgaatttg aagaaaattc ttgaaactat ctttcagggc ataagtgaag gaaccttttt
     3241 cccttaccgg gagaagagcc agggctgcag tttttgcgac ttcagaaatc tttgcgaaca
     3301 cggagtggat gtccttttcg aaaagaaaaa gaaggacccg agggcggcgg catttttgga
     3361 aattagacaa atcccatgac ggcaattgcg gattgcagag gggcgcggcg cgcgcccttg
     3421 agtcattctg ggtaggggct gggttcaaac cctctggttg gacgcttgca agcgaaactc
     3481 ggtgcaagat ggcgacaaaa atgttgggtc gccccggcgt ggttgcccat cttgcgagcg
     3541 gccacatagg gcctctccta caaaatcttg tctggcccgg cttgttcggg ttaggaagtt
     3601 ctctgttgaa atagaggtga attctaaatt gtctcttgcc gatgaggctg cacgagaaat
     3661 agcccgaaat gatcttgacc gctccctcgc cgtggaggcg ggggccggta cgggcaagac
     3721 cactcttttg gttgagcgga ttttgtcctt ggtccggaac cggcgggctc cgctggaaaa
     3781 gatcgtggcc atcactttca ccgaaaaggc agccggggaa ctgaaggtgc gtctgcggga
     3841 ggccatcgag aaggccctcc ccctttcgcc gccggcggag gccgagtctc tcggccaagc
     3901 cctgggcgaa ttggaacggg ctccgatatc caccatccat tccttctgct ccagccttct
     3961 tcgggaacgt ccggtggaag cttccctcga cccgaatttc gagcccttgg acgaaatggg
     4021 catggacctc ctcttccagg aggcctggga acagtggctt ggaaaggaga tggagaagaa
     4081 gcctgcggta ctgcgccggg ccatggccct gggcatggag atggaccatc tcgcccgcct
     4141 cgtgcggcaa gtttacgaga accgggacct cctccccgag ggtccattgc cgcagtcgtc
     4201 cttttcccca gagagcttta ttgaaatcct gggaaaagaa gttcaacgag cctgggactt
     4261 ggctcaatcg gattgccgga aagaagaaga cctggggtac caggccatcc tgggcctccg
     4321 gggaaaagcg aaagaattga aagaagcttc cccagatagg caagaagtca tcctcttccg
     4381 ggacttggag ataaaagccc aggggaataa gtccaactgg aagccgccgc cgagctgcga
     4441 cgcgcaaaaa gaaatcctca ataacctggc caaagagctg ggggccctca aggagaccct
     4501 tcgtgcccaa gtcatggccg gtctgatagg ctgggtcaaa ggatttgtgg ccgccatgca
     4561 ggaagaaaaa tccagaaggg gtgtcctgga ttttcaggac ctcttgatcc tttctcgaaa
     4621 cctgctgcgg gataacaaag aggttcgcga gtatttccaa aaaaatttcc agtacatcct
     4681 ggtggacgaa ttccaggaca cggaccctct gcaggtggag gtagtctttt tcctggccga
     4741 aaaaggagcc aaagcaaacc gctgggaaga ggtcgatctt cagcccggaa agctctttct
     4801 ggtgggagac cccaagcagt ccatctaccg gtttcggcga gcggatatcg aaacctatga
     4861 aaaggccagg gagagggtga tttccagggg ggcgggggta aacatcgtcc agaacttccg
     4921 gacggttcct tccatcctct cctgggtcaa ccgcatcttt tccggcctga tccagtcctc
     4981 gatggagggt acttaccagc cttcctatgt gcccctggtg cctcacccgg gacggaaaga
     5041 ggtcattaaa gcacaaccgg gggttcttct tctcgcgccc ccgccggatt tcgacccccg
     5101 ggaagcatcg gctgcaaaag taagggagga tgaagcccaa tccattgccg ccttgatcga
     5161 ggaaatggct gccggcggac ccgaagggag atggatggtc ttcgacaaga aggaagtggc
     5221 ccccaggccg gtatcttacc gggacatggc cttgctcttt cccgctctga ccgggatcga
     5281 cgcctatgaa gaagccctga aggatgggga aatcccctac cggcttgaag ggggaaaaga
     5341 attctacatg cgccaggaag tgcgcagtct cctctgctgt ctgaaagccc tcgacgaccc
     5401 ggcggaccgc atctccctgg tcgccgctct tcgctctccc ttcttcggtt tttccgatga
     5461 agagatcttc ctcttcagcg cttccgggaa ccggctaagc tatcttcacc acgccgaaga
     5521 agagaccggg gagttttccg aagccctgtc cctgatgagg gagctccata agaggaggaa
     5581 ttcccggccc atctccacca cggtctccga tctcctctcg cagaccaaag ctttggagtt
     5641 ctccctcctg cgtcagggag gagaccagat ggccgccaac ctccgaaagg tcctggagca
     5701 ggcccgggcc tttgaagggg aggcgcaggc gaactttcgg cggttcgtgg aatggctggg
     5761 aacccgggaa gaggagggcg tgcgcgaggg cgagtccccc tgggctgaag aaggtgagga
     5821 gaacgtgaag ctgatgacca tccacaaggc caagggattg gaatttccga tcgtattcct
     5881 ggccaacctg gcgagcgagg gtcggcgcag gcaggaattc atccccctga ggctccaggg
     5941 aacctttgaa atgcgcatcg gcaatttcca aaccaaagga tattcgtcgg ccctggagca
     6001 ggaaaaagcc aaaatggaag ccgaagatcg gaggctgttc tacgtcgccg ccacccgcgc
     6061 cagggactat ctggtccttc ccctcttttg ggggaggagg ggacgagggt tcttcagcct
     6121 ccttgaagaa aatttgcccg cctttgaatc gatggaacca tggtcggtga tcgatggaca
     6181 gctcattgcc ggccgggggt gcttcaacct ccgaccggcg gagaaaccac ccctgcgatt
     6241 ggacctggag gaatcggaaa aagaaaaaaa agaagagggg actccctggg aacgacgtct
     6301 tcaatggaaa gaatccttgg atgcggtcaa agaaaaggct tcc
//
LOCUS       DASPRR010000253         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_546155, whole genome shotgun sequence.
ACCESSION   DASPRR010000253 DASPRR010000000
VERSION     DASPRR010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546155"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VEL68_09870"
     CDS             <1..1553
                     /locus_tag="VEL68_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXZ36313.1"
                     /translation="NRKAPMEMDAEEFRRLGHWLVDHLADFLKSLPQRPVTPGISPGE
                     VRALLGNKPLPQRGIAAEKLLQEAAHLLFDHSLFNGHPRFWGYITSSAAPIGALADLL
                     ASAVNPNVGAWELSPVASEIEAQTIRWIAEMIGYPATCGGLLVSGGNMANFVGFLAAR
                     RAKVPWDTRTSGLGNGSMRLKVYVSRETHTWINKAVDMSGLGIDAIRWIPVNERRQMD
                     LRALEEQILSDRNKGLLPFLVVGTAGTVAVGAIDPLPDLAALCRRYGLWFHVDGAYGA
                     PAVILPDSPPELRGLREADSVALDPHKWLYSPLEAGCGLVREPNHLLEAFAFHPDYYN
                     FDDRGSEKPVNYYELGPQNSRGFRALKVWLALRMVGRDGYVRMIHDDIALAKKLYHRV
                     EASPELQAFTQNLSITTFRFVPLDLAAGGQLTENYLNELNREILNRIQTGGKVYVSNA
                     LLGDTFVLRSCIVNFRTRLADMDSLVEVVVQTGRSADARMRPQQLSKNMTSGRAGGMK
                     NSEPLKAD"
     gene            1875..>2400
                     /locus_tag="VEL68_09875"
     CDS             1875..>2400
                     /locus_tag="VEL68_09875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ36314.1"
                     /translation="MDNPISTFKFQRMSWMLTLRDLFVSPEEIMAEVDIQPGFRVMDY
                     GCGTGSFTFAAAHRVGPEGKVYALDLHPLAMEKVQKEALKKGLANIETVQTSCITQLA
                     SGTIDVVLFYYVLHWLTDPDCVLGELHRVLKESGILSFRDPYMKEEEILATVTGKGWF
                     RMSGKGVRTYRFIKV"
BASE COUNT          547 a          667 c          662 g          524 t
ORIGIN      
        1 aaaatcgaaa agcgccaatg gagatggacg ccgaggaatt ccgcaggctg ggacactggc
       61 tcgtcgatca tcttgcggac tttctgaaat ccctgcccca gcggccggtc actcccggca
      121 tttctcccgg ggaggtccgt gcccttttgg gaaacaagcc cttgccccaa agagggattg
      181 cggcagaaaa actgctccag gaagccgccc acttgctctt cgatcattcc ctgttcaacg
      241 ggcatccccg attctgggga tacatcacct cttcagcggc gccgatcggc gcgctggcag
      301 accttctggc gtcggctgtg aatcccaacg ttggagcctg ggagctttcc cccgtagcca
      361 gcgagatcga agctcagacc atccggtgga ttgcagagat gatcggttac cccgcgacct
      421 gtggcggact gctggtcagc ggagggaaca tggccaactt tgtggggttc ctcgccgccc
      481 gtagggctaa agtcccctgg gacacccgca ccagcggtct gggcaatgga agcatgcggc
      541 tcaaggtcta tgtttcccgc gaaacccaca cttggatcaa caaggccgtg gatatgtctg
      601 gcctcggtat cgatgcaatc cgatggattc ccgtgaacga gcggcggcag atggaccttc
      661 gggccctcga ggaacagatt ctatcagacc gcaacaaggg cctcctcccc tttctcgtgg
      721 tgggaactgc cgggacagta gcggttgggg ctatcgaccc tttgccggat ttggccgctc
      781 tctgccgaag atacggactc tggtttcatg tggatggagc ctacggcgct cccgccgtga
      841 ttcttcccga ttcaccgccg gaactgcgag ggctcaggga agccgactcg gtggccctcg
      901 atccccataa gtggctgtac agtcccctgg aagccggctg tggcctggtt cgggaaccca
      961 accatctgtt ggaagccttc gcctttcacc ccgactatta caatttcgat gaccggggga
     1021 gcgagaaacc ggttaactac tatgagctcg ggccgcagaa ttccaggggt tttcgggcat
     1081 tgaaggtttg gttggccctg cggatggtcg gccgggacgg ctatgtccgc atgatccatg
     1141 atgatatcgc tctggcgaaa aagctttatc accgcgtgga ggcttcaccg gaactgcagg
     1201 ccttcaccca aaatttgagc atcaccacct tccgcttcgt tccccttgac ttggcggccg
     1261 gaggccagtt aacggaaaac tatctcaacg aactgaaccg tgaaatcctg aatcgtatcc
     1321 agaccggggg aaaagtttat gtttcgaatg ccctcctggg agatactttt gttctgcggt
     1381 cctgcattgt caacttccgc acccgtttgg ccgacatgga ttcgttggtt gaagttgtag
     1441 tccaaacggg aaggtccgct gacgcccgga tgcgacccca gcaactctct aaaaacatga
     1501 cctccggcag agccggaggt atgaaaaaca gtgaaccgct caaagcggat tgaaaagggc
     1561 gccacccaaa ggggagcctc gatttggtgc caggccaagg ggcaaggacc ttctgggcaa
     1621 aaattaatag aatcctgcga agcgggaaaa attccagtcc caaaagaatg attcctgatc
     1681 gaagcaacat ctcgcttcta aaatccttcc aaaggtcatt cccatataaa taatcattaa
     1741 tgattctgca taattgtaat tcaaaaaaga tggcatgaat cttgttaatt accatatgac
     1801 gccggaattc taccctcccc tgagaagcat agggaataat agccggaatc catggaacaa
     1861 aggggatcgc cttcatggac aatccgatca gcaccttcaa atttcaacgc atgtcctgga
     1921 tgttgaccct gcgggacctc ttcgtctctc ccgaagagat catggccgaa gtggatattc
     1981 aacccggctt ccgggtcatg gattacggtt gtgggacggg gagcttcacc tttgccgccg
     2041 ctcaccgggt ggggcccgaa gggaaagtgt acgctcttga cctccatccc ctggcgatgg
     2101 aaaaggtgca aaaagaggcc ctgaaaaagg gattggccaa tatagagaca gtccagactt
     2161 cctgcataac ccagctggca tccggcacca tcgatgtggt tctgttctat tacgtcctcc
     2221 attggctcac ggaccccgat tgtgtcctcg gcgaactgca ccgcgttttg aaggagagcg
     2281 gtatcctatc tttccgggac ccctacatga aggaagagga gatcttggca accgtgactg
     2341 ggaaaggctg gttccgaatg tccgggaaag gggtcaggac ttaccgtttt atcaaggtct
//
LOCUS       DASPRR010000254        14559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_374502, whole genome shotgun sequence.
ACCESSION   DASPRR010000254 DASPRR010000000
VERSION     DASPRR010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14559)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374502"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..481
                     /locus_tag="VEL68_09880"
     CDS             113..481
                     /locus_tag="VEL68_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36315.1"
                     /translation="MEKVRAMGEKDEDLVVWWATRVECVSAVSRLRREGGLTAQAEQN
                     VRTALSTLAAGWSEVQPSDPFRHRAERLLMVHSLRAADAFQLAAALIWTEEETSGAEF
                     VCLDQNLRESALKEGFTVLP"
     gene            825..1319
                     /locus_tag="VEL68_09885"
     CDS             825..1319
                     /locus_tag="VEL68_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXZ36316.1"
                     /translation="MEEQGFIESREEMEELLREEEIGYLGLSLDGKPYVVPLNYSYSE
                     GKIYFHCGLKGKKLVCIRANPQVCFTVARQLAEVREHAGGNPCHVDSDSVICYGRARM
                     IEDIDERSKALNAFNRRFRANAADVPLERVRNCGLVEIKISEMTGRRERERKRTCWRY
                     VFPG"
     gene            1544..2512
                     /locus_tag="VEL68_09890"
     CDS             1544..2512
                     /locus_tag="VEL68_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36317.1"
                     /translation="MKARVFISLAAILVMTFANHAYPQGYPTKPVSLMVAYPAGGSTD
                     VGARIVAFIAEKELGQPLVVLNKAGAGGQVGWTELARQKPDGYYIGFINLPATNTVIL
                     DPDRKATFDIGSFTPIINQVLDPGVIYVKPDGPYKSFKDIIEDAKKRPGEVKVGTTGI
                     LSDDHLAILMLEEAAKVRFRIVHFDGDPPQLTALLGGQIDVSFLNVGGIAPKVKAGQV
                     RPLLVMDRERSKFYSDVPTSIEAGYPSVISSSTRGIVGPKGVPEPIVHKLQGVFKKAM
                     EDPEHMEKMDKAGLAVKIMMGDEYGKYIMSLHERIKRLVEEARKAR"
     gene            2951..3787
                     /locus_tag="VEL68_09895"
     CDS             2951..3787
                     /locus_tag="VEL68_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ36318.1"
                     /translation="MYHDLKGKVALITGAGKKTGIGFAIAEKMASCGSHIVIADLGQG
                     PETVDGMKMGLRPEMEGIARSLAEKYQVQTLAVDLDVTRPDSVQAMVQTVRVRFERVD
                     VLVNNAGASFGAPSPAHSYNEEAWMKTIDVNLHGTFRVSKAVLPLMTQKPVAIVNIAS
                     KAGKTPPLANGAYAVAKAGVIMLTKVMALELAKEGVRVNAICPGIIMTDLQVFRFQME
                     GRFLKQTPDEREKAWANDIPLGWVGAPSEVADLAAYLASKESRYITGQAINVDGGLLM
                     AL"
     gene            3935..5266
                     /locus_tag="VEL68_09900"
     CDS             3935..5266
                     /locus_tag="VEL68_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXZ36319.1"
                     /translation="MEQLTLPQKGRSKEEVLSLIRSFKARDSDWHDGHLFGLVYYAGQ
                     DVEDIAQEAYATYMFENALSPFDFPSLLKMETEFISMVSSLFHGDEATVGSMTSGGTE
                     SILMAVKAARDWARVHRPEVKAPEMVAPLTSHPAFNKAADYLGLRIIQTPVDGEFRAE
                     VEAVKEAITSNTIILTATAVTYPHGVIDPIAEIGALAAGKNLWFHVDACLGGYMLPFL
                     EKLGCEIPAFDFRVPGVRSMSVDIHKYGYVSKGVSTVLYRNLELRQHQFYVYTDWPGG
                     VYATPSLSGARPGGAIASAWSILHYLGEEGFLKLAKAARRATVKLVEGIQAIPGFFVL
                     GKPPATVFSFGSDKINVYQLAVKLKERGWHLHSQHRPASLHLTISPYHEKIVEPFLKD
                     LQEAASELAAAGPADSTGEAALYGMMGTLPDRKAAKALALQYLNNLYRLRE"
     gene            5379..6347
                     /locus_tag="VEL68_09905"
     CDS             5379..6347
                     /locus_tag="VEL68_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXZ36320.1"
                     /translation="MPEAGEDGVMVKISDTGFCGSDHSLIESGLLPDGIILGHEASGT
                     VFDRGSQKGALQEGTRVIVRPTYCGQCRECRMGKPHLCGIKRRSIGIGDLPGGFAEYV
                     KVFPPMLIPVPPGMDSRNAALAEAFASALHGVHCAENEGGSALVMGGGPIGLAAVRIL
                     KVLGFGPIALSEPVEEKRDLGKRFGADLVIDPLKEDVVGQGKEWTGGAGFETILECAG
                     VSENVSLAFTLAAKGGTICIVSLFKGLTVNQPMALNFKEPRLTWSYSNTHEENIQCLA
                     WMAEGKIDARDMITDLIPLEELPRVYRERIHPGKAIKVMMRIGEEF"
     gene            6509..7822
                     /locus_tag="VEL68_09910"
     CDS             6509..7822
                     /locus_tag="VEL68_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36321.1"
                     /translation="MATPSAPEGYLFSRGYTHYVFILLWLLYFFDYVDRMVVVSLFPF
                     LKSDWGLTDAQCGALVSAVYWAIVIFSFPISIVIDRWSRKKSIGIMAVLWSLATAACA
                     ITKSFGQLFATRTVIGLGEAGYAPGGTAMISALYPEKRRAFMVGLWNMSIPIGMAVGI
                     VAGGFIATHWGWKNAFGIVAFPGLLISILFFFVKDYKTVGLEQTVQRGAVQSSLLSAK
                     KRMSKMDIVRAFSRTPSLLLTYLGFAGMMFLTISLSSFFPTYFQRVHGAPLQKATLLT
                     SGIMLMAIIGSPLGGWIADRWMKSRVQARLLVSAISALLSAVLYFCAIHLFSGGVVQY
                     IILILGGIASMAWASSAIAVTQDVVHPGLRAISYALCVISQNLLGSSLGPIVTGALSD
                     AYGIAAALKFATVAALLSCVLFYLGSKFYKRDLEKVEKVALTAEK"
     gene            complement(8032..8559)
                     /locus_tag="VEL68_09915"
     CDS             complement(8032..8559)
                     /locus_tag="VEL68_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ36322.1"
                     /translation="MEPKSSSRKGHSPSGIRPLEPADRSAIEEMVISSGRFNAQEIAT
                     ALELVDEALEKGEASGYFFAVLEEEGQKSSIHGYACYGPTPLTQGVYDLYWIVVSPSA
                     QRKGYGRRLLEFVEKDIAGRGGRMLLIETSSQESYGGTVRFYQRAGYDLVARIKNFYR
                     VGDDKLIFSKNLIPL"
     gene            complement(8698..9888)
                     /locus_tag="VEL68_09920"
     CDS             complement(8698..9888)
                     /locus_tag="VEL68_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ36323.1"
                     /translation="MGPIGEGAALMLRVNRNCPWNQCLFCPVYKQSKFSPRSVTEVKR
                     DIDTARRVGDLLEGTSWEIGLNGYVQHEVIEACIKKHSGLYGEYPSRVTEKQWIALRC
                     LGNVANWYMYGARRVFLQDANALFMKPAELIKVLLYLKKTFPSVDTVTCYARSRTCAQ
                     RSAEEWTELHAAGLSWCFVGVESGCDGVLEFMKKGSSRKEQIEGGRKLMDSGVQMAAF
                     IMPGLAGKDAERSRRHTADTITVLNEIRPAEVRVRSLAVLKRAPLYGKWTAGEFLHPG
                     EDQMVEELKELIEGLTFDCTFETLQMTNAFTFQGRLPAQKNSFLDALGDYQSLPPLER
                     ARYLLNRYVRGGYLNFVQSWDSLDSTLQKKINEAEKSLEDESPEALEKVERVLGAIKS
                     KGIP"
     gene            complement(10116..11081)
                     /locus_tag="VEL68_09925"
     CDS             complement(10116..11081)
                     /locus_tag="VEL68_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXZ36324.1"
                     /translation="MKIQTIRLPESKKIEVIEADLPGPAEGQVLVKTYQSSICGTDRN
                     IYNGILPPGLKFPITMLGHEGGGTVMEVGKGVRKYKIGDRVMSCKKNGTFADYFLSLE
                     ENLHPIPTEMDLELGSLGEPLACVMYSIFNSGVQLGDTVAVMGLGFAGQLMVQGAKRK
                     GAITVVGIDPLAGKRDLAKKLGADQVFDPKIPDSEKAILEVTKGIGVDVVFEAAGTED
                     SMNMATRVLKKNGTLALYSWVMEPVRLNISRWHDDGMNIRTTCVMHVNTPYERFAWFD
                     RLLAPYWKGLIQVRPLLTGPFPPKEIPRAFETANRPDAIKVLIRG"
     gene            complement(11155..11970)
                     /locus_tag="VEL68_09930"
     CDS             complement(11155..11970)
                     /locus_tag="VEL68_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HXZ36325.1"
                     /translation="MPRKGKLGIWVLAAICLAISAFPPHVLAAKQPVSAVAASQELLQ
                     VKIQRLIIDPSSNQPVVFLTDLQEERALLVWIGPCEANAMNAEMEGTKHPRPQTHDLL
                     QGVIQKMKGKVQKIILTHSKDGVYYAALVLEREGTSIEIDARPSDSMVLALKAKAPVF
                     VARKMFQEMSVPLKEERGAEDQYGITAQELTSSLAQSFTFKSSGGVLVSDVRAGSPAE
                     TDGLQRGDIIAEIGGEATPDLKSLKTALNKMKGPVRARVFRKGEFISLTLHGK"
     gene            complement(12030..12998)
                     /locus_tag="VEL68_09935"
     CDS             complement(12030..12998)
                     /locus_tag="VEL68_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXZ36326.1"
                     /translation="MKQIETIRGARTIVETCAGVKAGESVLIITDTNMVGIAEVLAMA
                     VKERDAEPVLAIMIPRKGHGEEPPACLAEAMRKADVVLTPVSTSITHTRAMKEAAAAG
                     SRAIVMTAFTEDLLIQGGIQADFRKQKPICLEIARRFQEAKSVQLTSPAGTSLTMRKE
                     GRRGNALYCLVERGEFSTVPTIEANFSPLEGTADGILVADASIPYLGIGVLKEPVKCC
                     VGKGFIVGIEGGYQAEILKKDLESRKDPNSYNIAELGVGLNPESRMTGIMLDDEGVFG
                     SVHIGIGSNLTLGGNIKAAIHYDLLMWKATLELDGRVILGKGELKI"
     gene            complement(13025..13282)
                     /locus_tag="VEL68_09940"
     CDS             complement(13025..13282)
                     /locus_tag="VEL68_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36327.1"
                     /translation="MEEFKKILVLENEIEARLLDSVLNERKIPHRVRSYHDSAYDGIF
                     QTQKGWGVMLAPPEYEEEVLSIYQDLPLGEKGAAVENREEG"
     gene            13688..14416
                     /locus_tag="VEL68_09945"
     CDS             13688..14416
                     /locus_tag="VEL68_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXZ36328.1"
                     /translation="MKEKVLASSREFSPPVSIAEGVARFLESKIIRNELKPETRLIER
                     NLSETLGVSRISIREAFRILALSGLVKIIPRKGAQVTAITKQEVEEIYALRAYLVGLG
                     AKLAARNISREDLGELKKIASQMAEKSEKNDLRSYFSLNLKFHRLLSRVGGNQRLHQI
                     LENFGKQTHRFRYASLSLPGRMRKSSSYHQRLVEAIQKRDEERAEKIARTIIEEAGRA
                     LIEHPFDESSDFPELFKKTLPGNT"
BASE COUNT         3220 a         3984 c         3919 g         3436 t
ORIGIN      
        1 gacccgggcg gatcggtttt gaaggcactc ctggaagaaa gggagaaggg cagatgaggt
       61 tctgggattc ttcagcgatc cttcctctct gttcagtatg gaagaaccgg aattggagaa
      121 ggtcagggcc atgggggaaa aggatgagga cctcgttgtt tggtgggcga cgcgggtgga
      181 gtgcgtttcc gccgtgtcgc gcctgcgccg cgaaggggga ctgacagctc aagctgagca
      241 gaacgtaaga acggcgcttt ctacgcttgc cgcggggtgg tctgaggtac agccctccga
      301 ccccttcagg caccgggcgg agaggcttct catggtacat tctctgcggg cggcagatgc
      361 ttttcagctg gcggcggcat tgatctggac ggaggaggaa acttcgggag ccgagttcgt
      421 ttgtctcgat cagaatttgc gggaatcagc gttgaaggaa gggtttaccg tccttccgta
      481 aacatgaata gcctccgagc caggtcctct ctcccatgga atctctccgg gtcttaagag
      541 gttgcgattc cacgccccaa ttccaatttc caaaggaatg ccaccttccc cattcttttt
      601 gaatatcgag agccgggtcc accgatcggt cacaatcttc cccccttatt ggaaaatgta
      661 ggaagcttaa atcctatttg cttctccctg attatttcgg ttgaaatatt ccgtcaagag
      721 gtgtacgttt ctacccataa tcattaaggg ttgagcaaac ggctttaaga aattcaaaat
      781 agcgaatacg ctctttgctt cggggaaagg aaatggggga acaaatggaa gaacagggtt
      841 ttatcgaatc ccgtgaagaa atggaggagc ttttgcgtga ggaagagata ggttaccttg
      901 gcctgtctct ggatggcaag ccctatgttg ttccccttaa ttacagttat tctgagggaa
      961 agatctattt tcattgcggg cttaagggga agaaattggt ttgcataagg gcgaatccgc
     1021 aggtctgctt cacggttgca cgtcagctgg cggaggttcg cgagcacgct ggtggaaacc
     1081 cttgtcatgt ggacagcgat agtgtgattt gctacggcag ggcacgaatg attgaggata
     1141 tagatgaacg aagcaaggct ctgaacgctt tcaatcggcg tttccgggcc aatgcagctg
     1201 atgtaccatt ggagagggta agaaattgcg gactagtgga aatcaagatt tccgagatga
     1261 ctgggcggcg agagcgagag cgaaaacgca cctgttggcg gtatgtcttc ccgggctaat
     1321 gtgatagaga caaagtttga cctgacagac cttgtccgaa taatgaaatt attttgacaa
     1381 tattaatcta cgttcgcttt tctatgcgag agcgttatgg tcggcaatgt gggatgtcac
     1441 gctgtgcgtc tttttgtgcg acgagctttt tttgtggtaa aaacggggag aggttgggga
     1501 tgaacgaacg agctcgatta tcgtatgcta aagggggaga aaaatgaaag caagggtttt
     1561 catcagtctc gcagcgatct tggtgatgac attcgcgaat catgcctatc ctcagggtta
     1621 tccaacgaaa cctgtttccc ttatggtggc ctacccggct gggggaagca cggatgtggg
     1681 ggcccggatc gtggcgttta ttgccgagaa agaactgggc cagcctctcg tggttcttaa
     1741 caaagccggg gctggaggac aggtgggatg gacggagctg gccaggcaga agcctgacgg
     1801 ctactacatc ggtttcatca acctcccagc cacgaataca gtcattttgg accccgaccg
     1861 taaggctacc ttcgacatcg ggtcttttac ccccatcatc aaccaggttt tggacccagg
     1921 ggtgatctat gtaaagccag atggtcccta taagagcttc aaggacataa tcgaagacgc
     1981 caaaaagcgt cctggagaag tgaaggtggg aacaacgggt atcctcagcg atgatcattt
     2041 ggccatcctg atgctcgagg aggcggcaaa ggtcagattc cgtatcgtcc atttcgacgg
     2101 agatcctccc cagctcacgg ctcttctggg ggggcagatc gatgtgagtt tcctgaatgt
     2161 aggcggcata gcgccaaaag tcaaggcagg gcaggtaaga cccctgttgg tgatggatag
     2221 ggaaaggtca aaattttact ccgatgtgcc aacgtctata gaggcggggt atccctcggt
     2281 catctcttct tccactcggg gcattgttgg cccgaaggga gttcccgaac cgattgtcca
     2341 taagctccag ggcgttttca agaaggccat ggaggaccct gaacatatgg agaaaatgga
     2401 taaggcaggc ctggccgtca aaattatgat gggggacgaa tatggaaagt acataatgag
     2461 cctgcacgag agaataaaga ggctggtgga ggaggcccgg aaggctagat gagaaagagg
     2521 ggctaagatg gtttcacgat tatcatcgag agtcagggta cgctcggagg aactataatt
     2581 tataccctcc cgcgcgattg attcttgagg gtaggggaaa gagcaaaata aaagcccaga
     2641 ttgaatggcc tgggctcccc atcatgcagg aataggcatc ctttcagtta gtggtttctg
     2701 ggttctattt ttcgatctcc gagaagggat tgccaagaga ttgtacgccg cgacctcgat
     2761 atttcccata tggcctttct cattcacagc ctgagcctca tccttatggc ctcgcttgtt
     2821 tcttcctact tccagattcg ctttaaagag tacctggaaa taccgggaaa gctggattcg
     2881 aaaatcataa gagaacccgt gagcaatatg atccatttca tccaccagct ccaaaggaga
     2941 aaaccgagac atgtatcatg acctgaaagg aaaggtggct ttaatcaccg gcgcgggcaa
     3001 gaaaactgga atcgggtttg ccattgcgga gaagatggcc tcgtgcgggt cccatatcgt
     3061 gatcgccgac ctgggccaag gcccggagac agtggatggc atgaaaatgg gattgcggcc
     3121 ggaaatggag gggattgctc gaagtttggc tgaaaaatac caggtccaaa ccctggccgt
     3181 ggacctggat gtgacccgtc ctgattctgt ccaagcgatg gttcaaacgg tcagggtaag
     3241 atttgaacga gtggacgtcc tggtgaacaa tgccggggcc tccttcgggg caccctcccc
     3301 cgcccattcc tacaatgaag aggcctggat gaaaaccatt gatgtaaacc tccacgggac
     3361 tttccgcgta tcgaaggcgg ttctgcccct gatgactcaa aaaccagtgg ctatcgtgaa
     3421 catcgcttcc aaagccggga agactcctcc ccttgccaat ggagcctatg cggtcgccaa
     3481 ggccggagtg atcatgctga ccaaggtcat ggcccttgaa ctggccaaag agggcgtgcg
     3541 cgtcaacgcc atctgtcccg ggatcatcat gaccgacctc caggtctttc gatttcagat
     3601 ggaaggcagg tttctcaaac agactcctga tgagcgggaa aaggcctggg ccaacgatat
     3661 tcccctcggt tgggtcggag ccccttcgga agtggccgac ctcgccgcct acctggcgtc
     3721 gaaagaatct agatacatta ccggccaggc gatcaatgtg gatggcggcc tgctcatggc
     3781 cctgtgaatt gcggaatatt atcagtttcg agttccgggt ttcgagtttc aataagggct
     3841 cgggacgact tcttctttat tactcaagca aaatgggcaa ccacatcggg tcgcccagac
     3901 gatttaaggc tatgaaagat aaatgaggca gaagatggaa caattgacgt tgccccaaaa
     3961 ggggcggtct aaggaagaag tgctatcttt gatccggtcc ttcaaagctc gggattcgga
     4021 ctggcatgac gggcatctct tcgggctcgt gtattacgcc ggacaggatg tcgaggacat
     4081 agcccaggaa gcctacgcta cctatatgtt cgaaaatgcc ctcagcccat tcgactttcc
     4141 cagccttctg aagatggaga ccgaattcat ttccatggtc agcagccttt ttcatggcga
     4201 tgaggccaca gtcggctcta tgacttcggg aggaacggag agtattttga tggccgtgaa
     4261 ggccgcccgg gattgggcca gagtacatcg cccggaggtc aaggctcccg agatggtcgc
     4321 ccccctcaca agccacccgg ccttcaacaa ggcggctgac tacctgggtt taagaatcat
     4381 ccagaccccg gtggatgggg aattccgcgc agaggtggag gccgtgaagg aagcgatcac
     4441 ctccaacaca attattctga ccgccaccgc agtcacttat cctcatggag taatcgaccc
     4501 catagccgaa atcggggccc tggcagcggg aaagaatctc tggttccatg tggacgcctg
     4561 cctgggaggg tacatgctgc ccttccttga aaagctgggc tgcgagatac ccgcctttga
     4621 cttccgggta cccggcgtcc gttctatgtc cgtggatatt cacaaatacg gatatgtctc
     4681 caagggggta tccaccgtgc tgtaccgcaa cctcgaactg cggcagcacc aattctacgt
     4741 ttacacggat tggcccggag gagtttacgc gacgccgagc cttagcggtg cccgcccagg
     4801 gggagccata gcgtcagcct ggtccattct acactatctg ggagaggagg ggttcttaaa
     4861 gctggccaaa gccgcccgcc gggcgaccgt aaaactggtg gaaggaatcc aggccattcc
     4921 cggatttttc gtcctcggaa agcctcccgc aaccgtgttc tccttcggat cggataaaat
     4981 caacgtttat cagctggcgg tcaaactcaa agaaagaggc tggcacctcc attcccagca
     5041 ccgtccggcc agcctgcacc tgacgatctc cccataccac gagaagatcg tcgagccttt
     5101 ccttaaagac cttcaagagg ccgcttcgga gctggcggcc gccggaccgg cagactccac
     5161 gggggaagcg gcgctttatg gcatgatggg aactctgcct gaccgcaaag cggccaaggc
     5221 gctggctttg cagtatttga ataacctgta tcgcctcagg gagtgagctc ttatttccat
     5281 tctttaacct gaaactcgaa actctaaact tgaaactttt aagcatgaaa gccgctgtct
     5341 acagaaaaaa caaaggactg gttgtcgaag aaatccccat gccggaagca ggagaggatg
     5401 gggtgatggt aaaaatctcg gatacgggct tttgcgggtc ggatcactcc ctgatcgaaa
     5461 gcggactgct gcccgatggg atcatccttg gccacgaggc cagtggcaca gtcttcgacc
     5521 ggggcagcca gaaaggtgca ctccaggaag gaacacgggt tattgtccgg cccacttatt
     5581 gcggtcaatg ccgggagtgc cgcatgggaa agccccacct gtgcggaatc aagaggcgat
     5641 ccatcgggat cggggacttg cccggcgggt ttgctgaata cgtaaaggtc ttccctccta
     5701 tgctcatccc agtccccccg gggatggact cccggaacgc agctttagcc gaggcttttg
     5761 cttccgctct ccacggggtc cattgcgcgg agaatgaggg gggatcggct ttggtgatgg
     5821 gaggaggccc aatcggattg gctgccgtaa gaatcctgaa ggtcctcggg tttggaccta
     5881 tcgccctgtc ggaacctgta gaagaaaaga gggacctggg aaagcgattt ggggctgatt
     5941 tggtcatcga tcccttgaaa gaggatgtcg tcggtcaggg aaaggagtgg accggggggg
     6001 ccggttttga gaccatcctg gagtgtgccg gggtttccga gaatgtctcc ctcgcattta
     6061 ccctggcagc aaagggcggc acgatctgca tcgtgagctt gtttaaggga ctgacggtta
     6121 atcaacccat ggccttgaac ttcaaggaac ccaggctgac ctggtcctat tccaacaccc
     6181 acgaggagaa catccagtgc cttgcctgga tggccgaggg gaagatcgac gcccgggata
     6241 tgattaccga cctgatcccc ctggaagaac tgccgcgggt ataccgggaa aggatccatc
     6301 caggtaaggc catcaaggta atgatgagga taggagaaga attctagtct ataaatccga
     6361 atttcgaagc acgaaatccg agataaattg gaatgaacac aatttcgatg acccaaatcg
     6421 cctaaagaat gatgaccacc gagctttccc cttgcgcaat gcggtatgct ctatgcccta
     6481 tgggcgttat ctccatcgga ggtgcttcat ggccacccca tccgctcctg aaggctacct
     6541 cttctccaga gggtacaccc actacgtatt tattctcctc tggcttctct atttctttga
     6601 ttatgtcgat cggatggtgg ttgtctccct cttccctttc ctgaagagcg actggggatt
     6661 gaccgatgcg cagtgcgggg ccttggtctc ggccgtttac tgggccatcg tcatcttctc
     6721 tttccccatt tccatcgtga tcgaccggtg gagccggaag aagagcattg gcatcatggc
     6781 cgtcctttgg agtctggcca cagcagcctg cgcgatcacc aaaagcttcg gacagctttt
     6841 cgccacgcgg accgtcatcg gcctcgggga agccggatac gcccccggcg gtaccgccat
     6901 gatttcggct ctttatccag agaagaggcg ggccttcatg gtgggcctct ggaatatgtc
     6961 catccccatc ggtatggccg tggggattgt ggccggcggt ttcatcgcga ctcactgggg
     7021 atggaaaaac gccttcggga ttgtggcttt cccagggttg ctcatttcca tcctcttctt
     7081 ttttgtaaaa gattacaaaa cggtcggctt ggaacaaacc gtccagcggg gggccgtcca
     7141 gtcaagcctc ctttcggcaa agaaaaggat gtccaagatg gacatcgtcc gcgctttttc
     7201 ccgtaccccc tcccttctcc tgacctatct ggggttcgcc gggatgatgt tccttaccat
     7261 ctccctgtct tccttctttc cgacctattt ccaaagggta catggtgccc cgctgcaaaa
     7321 agccaccctg ttgaccagtg gaatcatgct tatggccatc atcgggtctc ccctgggcgg
     7381 ctggattgcc gatcgatgga tgaaaagcag agttcaagcc cggttgctgg tctccgccat
     7441 ctccgccctc ctgtctgcgg ttctctattt ttgcgccatt catttattct ccgggggagt
     7501 cgttcaatac attattctca tcctgggggg aattgcctcc atggcctggg cgtcatcggc
     7561 catcgccgtc acccaggacg tcgtccatcc cggcctgcgg gccatctcct acgctctttg
     7621 cgtgatttcg caaaacctcc tgggaagctc cctcgggccg atcgtgacgg gagccctttc
     7681 ggacgcgtac ggcatcgccg cagccctgaa gttcgccacg gttgcggctt tgctttcctg
     7741 tgttcttttt tacctggggt ccaagttcta caaacgggac ttggagaaag tagaaaaggt
     7801 cgctttgacc gcagaaaaat aggcttccga agggatgggt ttagaccgct ctatcagtcc
     7861 ctcagcaggt tctccccggg atcatccccc gatatggccg acatccgaaa cgggaggggg
     7921 catggattca ggatacgcag gaatggtaaa ccgaccttcc ccccttcgcc gaacaggtct
     7981 tgtcgagttg tttccagcca gtcagcgaca tggattctcg cgtccccctc cttagagcgg
     8041 aatcaggttc ttggagaaaa tgagtttgtc gtcccccacc cggtagaaat tcttgatgcg
     8101 cgccaccagg tcatacccgg ccctctgata aaatcggacc gttcccccgt agctctcctg
     8161 ggaagacgtt tcgatgagca gcatgcgtcc tcctcgcccg gctatgtcct tttccacaaa
     8221 ttccaacagg cgccttccgt acccctttcg ctgggccgaa ggacttacca cgatccagta
     8281 gagatcataa accccctggg tgaggggagt cgggccgtaa caggcgtacc cgtgtatgga
     8341 ggacttctgc ccttcttctt ccaggactgc gaagaagtat ccgctggcct ctcctttttc
     8401 cagggcctca tccaccaatt ctaaagccgt ggcgatttcc tgggcgttga atctgccgga
     8461 ggagatgacc atctcctcga ttgctgagcg gtcggccggc tccaatgggc ggattcccga
     8521 tggggaatgt cctttccgcg atgatgactt cggttccatg ctctcttttc cttcttaccc
     8581 gcagtatcaa gcatgatccc ttgaggaggt ggacgggtaa accagctccg gcggctcgcc
     8641 tttccccgcg gttattcacc cccagcaaag ccgcgagtct gccgaaagga atggatttca
     8701 aggaattcct tttgatttaa tggcccctaa gaccctttcc actttttcca gggcttcggg
     8761 ggattcgtct tccaagcttt tttctgcctc attgattttc ttctggaggg tggagtccag
     8821 ggaatcccag gactggacga aattcaagta tcctccccgg acatagcggt tcagcaggta
     8881 tcgggccctt tccaacgggg ggaggctctg gtaatcgccg agggcatcga ggaaagaatt
     8941 tttctgggcc ggaagcctcc cctgaaaagt gaaggcattg gtcatctgca gagtctcaaa
     9001 ggtgcagtca aaggtgagac cctctatcag ctcttttaat tcttccacca tctgatcttc
     9061 acccggatgg aggaattccc ctgccgtcca tttcccgtaa agaggagccc ttttcagtac
     9121 ggctaggctg cgcacccgca cctcggcggg ccgaatttcg ttgaggacgg tgatggtatc
     9181 cgcggtatgc ctccgggacc gttcagcatc ctttcctgcc aggccgggca tgataaaagc
     9241 ggccatctgg acacccgaat ccatgagttt ccgtccgccc tcaatttgct ccttccggct
     9301 ggaacccttc ttcatgaatt ccaacacccc atcacacccc gattcgaccc ctacaaaaca
     9361 ccaggaaagc ccggcagcat gcagctccgt ccactcttct gcggatcgct gggcgcaggt
     9421 cctagaccgg gcataacagg taaccgtgtc caccgagggg aaggtctttt tcaaataaag
     9481 cagaaccttg atcaactccg ccggcttcat aaatagggca ttggcgtcct gcagaaacac
     9541 ccggcgtgcc ccgtacatat accagttggc cacgttgcca aggcagcgga gcgcgatcca
     9601 ctgtttttcc gtaaccctgg atggatattc tccatacaac cccgagtgtt ttttgatgca
     9661 agcctcgatc acctcatgct ggacataacc gttcaacccg atctcccagg acgtaccttc
     9721 cagcaggtcc cccaccctgc gggccgtgtc aatatccctt tttacctcgg ttaccgaacg
     9781 cggggaaaat ttgctctgct tgtaaacggg gcagaagagg cactggttcc aggggcaatt
     9841 ccgattcacc cgcagcatga gtgcggcccc ttccccgatg ggacccatgg ggccaagttc
     9901 caggatgtaa ttttcagggt tcttttcagg catgggaaaa tagggcgaga cgacagagct
     9961 ttattcaggc tgatgctccc aaaaacagag cactaaatta gaaaaccaca aagcccatag
    10021 tgttcggcgc aaagcgcttt acaaaagcca ttatttattt tttcattctc ttatcgctag
    10081 gatctatgct ccatgtgcta tgcgtcttta ttctcttagc ctctaatcag caccttgatc
    10141 gcatccggcc tattggccgt ttcaaaagcc ctgggaattt ccttcggcgg aaagggcccg
    10201 gtcagcaacg gtctcacctg aatcaagccc ttccagtaag gggccaggag gcggtcgaac
    10261 caggcgaacc tctcataggg ggtattcacg tgcatgacgc atgtcgtgcg gatgttcatt
    10321 ccgtcatcat gccatcggct gatgttgagc cgaaccggct ccatcaccca gctgtaaagg
    10381 gcgagagtcc cgttcttctt caagacccgg gtggccatgt tcatggagtc ttccgtgccc
    10441 gcagcttcga agactacatc caccccgatt cctttggtca cttccaggat ggccttctcc
    10501 gagtcgggaa ttttggggtc aaagacctgg tctgccccca gttttttggc caaatctcgc
    10561 ttcccggcaa gaggatcgat cccaacaaca gtgatggccc ccttcctttt ggccccctgg
    10621 accatgagtt gcccggcaaa acccagcccc atgaccgcta ctgtgtcccc gagctggacc
    10681 ccggagttga agatcgaata catcacgcag gcgaggggtt cccctagaga ccccagttcc
    10741 aggtccatct ctgtcgggat gggatgtagg ttttcttcca aagataaaaa ataatccgca
    10801 aaggtcccgt tcttcttgca ggacatcacc cggtcgccga tcttatattt ccgaactcct
    10861 tttcccactt ccataaccgt gcctcccccc tcatgcccca gcatggtaat ggggaatttc
    10921 aaccccggag ggaggatgcc gttgtaaatg ttccggtccg taccgcaaat ggaggattga
    10981 taggtcttta ccagtacctg tccttctgcg ggtcccggaa gatctgcttc aatcacttcg
    11041 atttttttcg actccggcaa gcgaatggtt tgaatcttca ctcgatttca cctttctgca
    11101 taataactcg atcggaattc aggcaaacgc ttgaatcggg tatagtcccg tcggtcattt
    11161 cccatggagg gtcagagaga tgaattctcc cttgcgaaac accctggccc tgaccggccc
    11221 cttcatttta ttcagagcgg ttttcagact cttcaaatcg ggagtagctt ctcccccgat
    11281 ttcagcgatg atgtctcccc gctgcaggcc gtctgtttcg gcggggctcc cggcgcggac
    11341 gtcggaaacc agcacccccc cgctcgattt aaaggtgaag gactgggcca gggaagaggt
    11401 cagttcctgc gcggtaattc catactgatc ctcggccccc ctctcctctt tcaagggaac
    11461 cgacatttcc tggaacattt ttctggcgac gaagactggg gctttggcct tcagggccaa
    11521 gaccatggag tcgctgggcc gcgcgtcgat ttcgatggag gtgccttccc gttccagcac
    11581 cagggccgca taataaacgc catctttgga atgggtgagg ataatcttct gcacctttcc
    11641 cttcatcttc tggatgactc cctgaagaag gtcatgggtc tgggggcggg gatgcttggt
    11701 cccttccatt tcggcattca tggcgttggc ttcgcagggg ccgatccaaa ccaagagcgc
    11761 gcgctcttcc tggagatcag tgaggaagac cactggctgg ttgctcgaag ggtctatgat
    11821 caaacgctgg attttgactt gaagcagttc ttgggatgca gccacggcag atacaggttg
    11881 tttggccgcc aatacatgag gcggaaaggc agagattgcc aagcagattg ccgccagaac
    11941 ccaaatgccc aattttcctt ttcttggcat atccccctcc ttccggatga aacgccaaat
    12001 cccaattctg cgaaaagcgg ggttgggatt catattttca gttctccctt gcccagaatg
    12061 acccttccgt ccagctcgag ggtggccttc cacatgagga gatcgtaatg aatggctgcc
    12121 ttgatatttc cccccaaggt gaggttggac ccaatgccaa tgtgaaccga accaaagact
    12181 ccttcgtcat ccagcatgat cccggtcatc ctggattcgg ggttcaggcc cactcccaat
    12241 tcggctatgt tgtaactgtt ggggtctttc ctggactcca ggtctttctt taatatttcc
    12301 gcctggtacc cgccttcgat tcccacgatg aacccttttc ccacgcaaca ttttaccggc
    12361 tccttgagga ccccaatgcc caggtaggga atgctggcat cggccacaag aatcccatcg
    12421 gccgttccct ccaagggaga aaaattggcc tcgatggtcg gtacggtgga aaactcccct
    12481 cgctccacca aacaatagag ggcattgcct ctcctgcctt ctttgcgcat cgtaagggat
    12541 gttccggcgg gggaggtcaa ctgaaccgat tttgcctcct gaaaacgcct ggctatttcc
    12601 agacagatag gcttttgttt cctgaaatcc gcttggattc ctccctgaat caaaaggtct
    12661 tcggtaaagg cggtcatgac gatggcccgc gagcccgctg ccgcggcctc cttcatggcc
    12721 cgggtgtggg taatggaggt gctcaccggg gtcaggacca cgtcggcctt cctcatggcc
    12781 tctgccaggc aggccggggg ttcttcgccg tggcctttgc ggggaatcat aatcgccagg
    12841 accggctctg cgtccctttc tttcaccgcc atggccagca cttcggcgat tcccaccata
    12901 ttcgtatccg ttatgatgag aacgctttcc ccggccttca ccccggcgca ggtttctacg
    12961 atggttcttg ccccgcgaat ggtttcaatc tgcttcatgg tctccttcct aatccaaaaa
    13021 gagttcagcc ctcctctcgg ttttcaaccg ccgccccttt ttctcccagg ggcaggtcct
    13081 ggtagatgga caggacctct tcctcatatt cgggcggggc cagcatcacc ccccagcctt
    13141 tctgggtctg gaagatcccg tcataggcgg agtcgtggta acttcggacc cgatggggga
    13201 ttttccgctc gttgagcacg gagtcgagca gtcgtgcttc aatctcgttt tccaggacca
    13261 agatcttttt gaattcttcc atgtgaaagc cgctccatcc tgattttaaa cctttcgggt
    13321 gattcaatcc ccccggcgct ggcctccagg gacatcttct cgcagaaccc atcgaccctg
    13381 tttaaccgtt cctcccgcgg gcgaccccgg ggagtcgacc ttacgatgct gcccccttat
    13441 tatgcggggt agatcgagaa gggagaaatt tcgctctcac catctcccct agcgcccttc
    13501 atcctcccct actcttaaaa tattagtgaa atcctctctg gccagcaaca gaaatatcca
    13561 tcccttcctt tttcctattt ttcccggatt ttttatcttg acacccccag ttctcttctg
    13621 gtacagaagg ggatagattt ggtataccat ataccattta gcatccatcc gactttgagg
    13681 aaaacctgtg aaagagaaag tcctggcatc ctcccgggaa ttttctcccc ccgtctccat
    13741 cgccgaagga gtggcccgat ttctggaaag caagatcatc cggaacgagc tgaaacctga
    13801 gacccgtctg atcgaacgga atctctccga aaccttgggg gtcagccgga tttccattcg
    13861 tgaagctttc aggattctgg ctctgtccgg gctggtcaag atcatccccc gaaagggagc
    13921 ccaggtcacg gccatcacca agcaggaagt agaggaaatc tacgctctgc gggcctacct
    13981 ggtggggctg ggggccaagc tggcagccag gaacatcagc cgggaagacc tgggagagct
    14041 caaaaagatt gccagccaaa tggcggagaa aagcgagaag aacgacctga ggtcctattt
    14101 ttctttgaac ttgaaatttc atcgcctcct ttcccgagtc ggggggaatc aaagattgca
    14161 ccaaatcctg gagaatttcg ggaagcagac gcaccgtttc cggtatgctt ccctgagcct
    14221 cccgggaagg atgcggaaat ctagctccta tcaccaaagg ctcgtggagg cgatccagaa
    14281 gcgggatgaa gaaagggctg aaaaaatcgc ccggacgatc atcgaagaag ccggtcgggc
    14341 gctgatcgag caccctttcg atgagtcatc ggacttcccc gagcttttta aaaaaacgct
    14401 ccccggaaac acctgaaccg gaaaatggac gcttcagggg atttcgccgt ctcctgcctg
    14461 aaagacgggc tttcttctca tgttacccaa agggatcgaa ttcatgcggt tctacctaaa
    14521 cattcctttc caaggaggtc gctatggctg aaatactga
//
LOCUS       DASPRR010000255        13295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1185415, whole genome shotgun sequence.
ACCESSION   DASPRR010000255 DASPRR010000000
VERSION     DASPRR010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13295)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13295
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185415"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..755)
                     /locus_tag="VEL68_09950"
     CDS             complement(291..755)
                     /locus_tag="VEL68_09950"
                     /inference="COORDINATES: protein motif:HMM:NF044627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36329.1"
                     /translation="MNEALRGTLAEVEKELLSLQELLDELAVVKTQTDVIAKRAKGSI
                     LHDFYNCCERIFKKIAVEMNSGYEESEKWHKGLLYRMTIPVEGIRPRVISDELAADLD
                     EFLAFRHLFRNIYGYELKGGRIDFLAGRLEEVAGRFKKEVREFVAILKQELD"
     gene            complement(752..1084)
                     /locus_tag="VEL68_09955"
     CDS             complement(752..1084)
                     /locus_tag="VEL68_09955"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXZ36330.1"
                     /translation="MGYVAWFKKKAGEKEALRKEAFKEAQRLSALLHKEFPFEALYLI
                     GSVIKGKGFTRHSDIDFVVRGLEEDLFFKAFAFLLKNSRFDIDLKPWEELDEESKKTV
                     EREGGILS"
     gene            complement(1133..1690)
                     /locus_tag="VEL68_09960"
     CDS             complement(1133..1690)
                     /locus_tag="VEL68_09960"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HXZ36331.1"
                     /translation="MGQGDETQEIKSGSIEELSLWYCIHTRSRHEEVVFLRLEDKRMN
                     AFLPKLEVWSRRKDRRKKIQKALFPGYLFVHEILDPFHRLEILKTPGVVRILGNEKGP
                     VPVPDTQIESIRTILNGKSAVSPFPYLKEGQIVRVVEGPLKGCEGFLLKIKEGREKLI
                     ISVDLLQRSVAVEIDGASVEPVSLL"
     gene            complement(1764..3458)
                     /locus_tag="VEL68_09965"
     CDS             complement(1764..3458)
                     /locus_tag="VEL68_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HXZ36332.1"
                     /translation="MEIQKPEKTDLGASEPPVSVCPAGRVFPLPQKEDYDREVERLRK
                     VVEEQRALGREIVLVMGVGFVGAVMAGVVADSVNPSNGRPSKFVIGMQRPSARSYWKI
                     PYLNEGMAPVEAEDPEVAPMIRRCVLEKKTLMATFTHDALSLADVVVVDVQCDYQKES
                     LGNVEDGYADIKALEESLRIIGEKIQPDCLVLIETTVPPGTTEYIAYPLIKNGFERRG
                     LKEAEPLIAHSFERVMPGRDYVSSIRDFWRVCSGINPASRERARKFLSDVINVKKYPL
                     TVLDRPIESETCKVVENSYRATILAFLHEWSLFAERNGVDLIKVLEAIKVRPTHSNII
                     FPGPGIGGYCLPKDGGLGVWAYRTLLGFEDQIFRITPLAININDTRALHVAEIVRDAL
                     RNMGRIVAASRIAVLGASYREDVGDTRYSGSEVIVRKLTEMGAEIVVHDPYVQHWWEF
                     EKQDSYPAPGKSWARFFRNQEKLTELRIQQNLAKSLESVDAVVLAVRHQAYRKLTPDE
                     ILRMSGRPIAVIDCFGILDDSKISRFFELGCEVKGLGRGHVKRLKDEAREKRMNRR"
     gene            complement(3761..4600)
                     /gene="rfbD"
                     /locus_tag="VEL68_09970"
     CDS             complement(3761..4600)
                     /gene="rfbD"
                     /locus_tag="VEL68_09970"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HXZ36333.1"
                     /translation="MKILIIGARGMLGRDLQPILSPRHEVIGTDLDELDITRPGKLRE
                     ALDSLRPDVVINLAAVTDVDGCESQRERAFLVNAHGALEVARGCAATGARLIHLSTDY
                     VFDGTSPVPYTEEILPRPLNVYGESKLLGERGVQETGGNYLILRTAWLYGQHGKNFVD
                     TILRLSSQQEEIRVVNDQRGSPTYTRDLSRAIELLLKREERGVLHVTNSGSCTWFEFA
                     GKILALKNPRDPRVRLLPISSAELGRPAKRPANSVLDCSKFEIIAGWKMRRWEECLKE
                     YIS"
     gene            complement(4597..5619)
                     /gene="rfbB"
                     /locus_tag="VEL68_09975"
     CDS             complement(4597..5619)
                     /gene="rfbB"
                     /locus_tag="VEL68_09975"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HXZ36334.1"
                     /translation="MKVLVTGGAGFIGSNFIRLLLGLEKRGKYQVVNLDKLTYAGNLE
                     NLSDLSSLEGYRFVRGDIGDGKLVESLVHEGVEAIVNFAAESHVDRSLYEPDLFLRTN
                     VLGVQVLLQAALRHKVRRFVQISTDEVYGSLSSGEPPFREDHPLAPNSPYAASKASAD
                     LLVRSYFKSYGFAAVITRCSNNYGPYQFPEKLIPLMITNALEGKTLPIYGDGLNIRDW
                     IHVEDHCRAILLVLEKGRGGEVYNIGGNGERSNLEIVEEILRILKKPKTLLRYVADRP
                     GHDRRYGIEFSKLKGELGWSPSISLEEGLAQTIRWYLDREEWWRRIKSGAYREFYQKH
                     YGEGLP"
     gene            complement(5757..6212)
                     /locus_tag="VEL68_09980"
     CDS             complement(5757..6212)
                     /locus_tag="VEL68_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HXZ36335.1"
                     /translation="MIEGVQIKRLKVISDERGRLMEMLRADDPIFIRFGQVYMTTAYP
                     GTVKAWHYHKKQFDHFVVVKGMMKVVLYDGREKSPTFREINEFFMGEHNPILLQIPPF
                     VRHGFKCISEEEAIVVNCPTEVYDSQSPDEYRLPPHGGEIPYDWSRKDG"
     gene            complement(6279..6650)
                     /locus_tag="VEL68_09985"
     CDS             complement(6279..6650)
                     /locus_tag="VEL68_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXZ36336.1"
                     /translation="MNIMEKPHKRLDAWKESVTLVTLVYELTRQFPRNEVFGLTSQLR
                     RAVISIPGNVAEGAARQTRREFVQFLYIARGSLSEVDTYIEIARRLGYIEKGSIAMVE
                     EKMVDVDKIITGLIKSLKSKN"
     gene            complement(6723..7451)
                     /locus_tag="VEL68_09990"
     CDS             complement(6723..7451)
                     /locus_tag="VEL68_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXZ36337.1"
                     /translation="MKGVILAGGLGTRLYPLTKITNKHLLPVFDKPMIYYPLQALVNS
                     GIRDILIVTGGNHAGDFLKLLGNGKEFGLQHVNYTYQEGEGGIAAALDLAEYFSEGEK
                     ICVILGDNIIEKNIVHAVRAFEKQERGAKILLKEVPDAHRFGVPEVRDGRILRIEEKP
                     QDPPSNYAVIGIYMYDPEVFRIIKTLKPSDRGEMEITDVNNEYLRRGTLTYDVLEGWW
                     TDAGTFESLLRASRLVAETGANKL"
     gene            complement(7488..8429)
                     /locus_tag="VEL68_09995"
     CDS             complement(7488..8429)
                     /locus_tag="VEL68_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HXZ36338.1"
                     /translation="MRVLVTGGAGFIGSHLCDRFLQEGHEVICLDNLITGSTDNIAHL
                     AGQSKFSFIKHDVTNYIFVEGKIDAILHFASPASPVDYLQYPIQTLKVGSLGTHKALG
                     LAKEKKAIFLLASTSEVYGDPLIHPQKEDYWGNVNPIGPRGVYDEAKRFAEAMTMAYH
                     RYHGVDTRIARIFNTYGPRMRLNDGRVVPNFICQALRGQDMTIYGDGFQTRSFCYIED
                     QVEGMIRLLFSQEIEPVNLGNPQEYSIQDFAQVILQLTGSKSRIIYQSLPTDDPRVRK
                     PDISRAQAVLGWNPKVELKEGIGKTIPYFQGKLEKEN"
     gene            complement(8426..9784)
                     /locus_tag="VEL68_10000"
     CDS             complement(8426..9784)
                     /locus_tag="VEL68_10000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXZ36339.1"
                     /translation="MESMNLCVTGTGYVGLVTGTCLAEIGHRVTCLDNDESKIQTLDA
                     GKMPIFEPHLEDLIRDNREKGRLSFTTDFGMAFQDAEGIFICVGTPPLENGEADLSAV
                     EKVVREIASRSKSYKLVIEKSTVPVQTGEYILRTLQMYCRGKKDLFDVASNPEFLREG
                     SAVNDFFHPDRIVVGVDKERAGKTLRELYQPILERNFSCPVHSPCPPSSLPAFLVTDV
                     KSAELIKHASNSFLAAKISFINAIADLCEKVGADVDKVAQGMGLDARIGKDFLRAGLG
                     FGGFCFPKDLQAFVRIAEKNGYDFSLLREVEKINKGRIEVALEKLRRELWVLQGKTIG
                     VLGLAFKPQTDDTRFAPALELIRRLLREGVKVRAYDPKAAEKTREEFPDIFYAQSAFE
                     AAEGTEALILATEWTEFRQLDWGKIRQVMIHPYIFDGRNFLDSMQLRSLGFRYEGMGR
                     RQ"
     gene            complement(10020..11027)
                     /locus_tag="VEL68_10005"
     CDS             complement(10020..11027)
                     /locus_tag="VEL68_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ36340.1"
                     /translation="MILVTGGAGFIGSHLVEALLHRGQKVRILDDLSTGKMENLEEAT
                     GFRVPPLDAGPGRTPLISLGEKAEFLRGDISDEETCRKACEGVSCVFHEAALGSVQRS
                     VEDPLTTHRVNATGTMNMLWAAKEAGVKRFLYASSSSVFGNLLSNPEEAVPKEEADPL
                     HPESPYAATKLMGELYCRIFACIYGLETVSLRYFNVFGPRQDPNSIYAAVIPRFMDAL
                     LHGAQPVIYGDGNQSRDFTFVENVVQANLLAMEAEGVSGKVFNIACGQGISVNHLLSR
                     LQAISGKKIPAQYEAARSGEIRHSLSSTRLAQRDLGFEPRVPFAEGLAVAWHWFQKRG
                     I"
     gene            complement(11024..12121)
                     /locus_tag="VEL68_10010"
     CDS             complement(11024..12121)
                     /locus_tag="VEL68_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXZ36341.1"
                     /translation="MSFWVEKLFHLLIHRKWNLDKGTFDPGQVQRILVVRNDNVGDVL
                     CSTPAIRALRRAFPRAYLAALVVAYSRDAVTGNPDLDEVLVYEKAKHRPDRNHMVSLF
                     KQLQVEWDLRRKGFDLVIGMRSSFSWSEAWIVYFTGAPFRLGYDPGVGKNHKFSFFYN
                     LRVPKPPEQHEVEKALFLVQRIGVQPSEKSLKVFIPQEEKDRVDAFLKGNEIDPERLI
                     GFHLSSRLPANGWGPEKFSELALRIIQEKGKTVILTWGPGDEGRAKEVQRRVGAGVFL
                     YPTPNFKSLGAIQQRCRLFVSPDGGAMHFSTAVGTPTVGLFGKTDPRLWAPWGDGNIA
                     LRRGKESELISVDEVYKTMIGILSEGRKEVL"
     gene            complement(12118..>13295)
                     /locus_tag="VEL68_10015"
     CDS             complement(12118..>13295)
                     /locus_tag="VEL68_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ36342.1"
                     /translation="NPLRIIYPIHEGVDFRRARFHQVFQTVHALARRNCEVDLIIGRN
                     RVDVLSEGLSHYGLWPHENLRIHLLPMLRREEDRFLRISWDGVFLWACLVKVRELLRQ
                     RAYDAIFIRHLNTVSFFLRFKRYLHLPMLFEAHEIFFLTTDHHGKMEKIKREEFKIYP
                     RLDGIISITRGLARQMKEVFSLQIPMAVVPDGVNLDFFGGIARKPRNQKIIYVGQLYP
                     WKGTGTLVEAMQYLPIGELHLIGGSEERIQALREKAARLGVQDRVFFLGQLPPREVKR
                     HLADSSVAVLPLTQDLISASFTSPLKLFEYMAARIPIVASDLPSTREVLSSGVNALLV
                     QPNDPRALGEGIRRLLEDRSLAEGLGQKAYEDVQEFTWAKRAQKISHFIQSLPREES"
BASE COUNT         2908 a         3791 c         3459 g         3137 t
ORIGIN      
        1 gaatggtttc ggtatgggcg ataggcagaa aaatcaccag gacacagagg cagaaaagaa
       61 ttatcggatc gataatctga ataatcctat ctcgttccat gcatggcctt tattccggaa
      121 atcatttcta agatcaaccc aaaatataat aatgtcgcca ctcgagattg tttgttaatc
      181 cgttgtaagg gagaaccaag ggcaagatcg gtgacccagt atattttgat tggcccgtcg
      241 atcgagtcgg ccaggcaccg ctgcagggaa tttcaaggag aaaccagtag tcaatccagc
      301 tcctgcttca aaatcgcgac aaattcccgg acctcttttt taaaccttcc agcgacttcc
      361 tccagtcttc ccgcgaggaa atcgattcgc cctcctttca attcgtagcc ataaatattt
      421 cggaaaaggt ggcgaaaggc caagaattcg tccaggtcgg cggcgagttc atccgatata
      481 accctcggcc ttatcccctc gacgggtatg gtcattcgat aaagaagccc tttgtgccat
      541 ttctcggact cttcatatcc gctattcatt tccaccgcga ttttcttgaa aatcctttcg
      601 caacagttgt aaaaatcatg caggatggac cctttcgccc tcttggcaat cacgtcagtc
      661 tgcgttttca cgactgccag ttcgtccaat agttcctgaa ggctcagcag ttccttttcg
      721 acctcggcta gggtccctcg aagggcttca ttcacgaaag gattcccccc tctctttcca
      781 ctgttttctt gctttcttca tccaattctt cccaaggttt gagatcgata tcgaagcggc
      841 tgtttttcaa caagaaggcg aaggctttga aaaagaggtc ttcctcaaga ccccgaacca
      901 cgaaatcaat atcggaatgc cgggtaaacc cttttccttt gatcacagaa ccgattaaat
      961 agagggcttc aaacggaaat tccttatgaa gaagggcgga aagcctctgc gcttccttga
     1021 aggcttcttt cctcaacgct tctttctctc ctgctttttt tttgaaccag gctacatatc
     1081 ccatttttcc ccggtcattc aggttcttcc tatcgtagcc tttctaatca tattacagca
     1141 ggctcaccgg ctccacgctg gccccgtcga tctccacggc cacggaccgc tgcaggaggt
     1201 ccacggaaat gatcagtttt tctctccctt ccttgatttt caggaggaag ccttcgcacc
     1261 ccttcagggg tccttccacc acccggacta tttgtccttc cttcaggtaa ggaaagggag
     1321 aaactgcaga ttttccattc aggatagtcc ggatggactc aatttgcgta tcgggcaccg
     1381 gaaccggacc cttttcgttt cccaggattc tcaccacccc gggagttttc aggatttcca
     1441 gccggtggaa ggggtcgagg atttcgtgga cgaaaaggta accaggaaag agggcttttt
     1501 ggattttctt ccggcgatcc ttgcggcggc tccagacctc gagtttgggg aggaaagcat
     1561 tcatccgttt gtcctccagc cgcaggaaaa ccacttcttc gtgccggctc cgggtatgaa
     1621 tgcagtacca gagggacaat tcttcaatcg aaccgctttt tatctcctgt gtttcgtcgc
     1681 cctggcccaa aaatcacctc tcccgatttc tctactttcc ccacagttgg cggcaaaaag
     1741 ccacctcccc aactatcttt ctttcacctg cgattcattc ttttctcccg ggcctcgtct
     1801 ttcagtcttt tgacgtgccc tcgccctaga cccttcacct cgcaccctag ctcgaaaaag
     1861 cggctgatct tggaatcatc caatattcca aagcaatcga tcaccgctat aggccggccg
     1921 ctcatgcgga ggatttcgtc gggggtgagc tttctatagg cctgatggcg gaccgctaga
     1981 acgacggcat ccacgctttc cagtgatttg gcaaggttct gctggattcg caattcagtc
     2041 agcttctcct ggtttcggaa gaaccgggcc caggattttc ccggggccgg ataagagtcc
     2101 tgcttttcga actcccacca gtgctgaaca taggggtcat ggaccacgat ttccgccccc
     2161 atctcggtca atttgcggac gatgacctcc gacccactgt agcgtgtgtc ccccacgtct
     2221 tcccggtagg atgctcccag cacggcgatc ctggaagcgg ccacgatccg ccccatgttt
     2281 ctcagggcat ccctcacgat ctccgccaca tgaagggctc gggtgtcgtt gatgttgatg
     2341 gccaagggag tgatgcggaa gatctgatct tcgaacccca gcagggtgcg gtaggcccag
     2401 acccccagac ctccgtcctt gggaagacaa tagcctccga tccccggtcc gggaaagata
     2461 atgttggaat gagtggggcg aaccttgatg gcctccagga ctttgatcag gtcaacgccg
     2521 ttccgctcgg caaagaggct ccactcgtgg agaaacgcca aaatagtggc gcggtaggaa
     2581 ttttccacca ccttgcaggt ctcgctctct atggggcggt ccagaacggt caggggatac
     2641 tttttcacgt tgatcacgtc ggacagaaat tttctcgctc gctcccgaga ggccggattt
     2701 atcccgctgc agaccctcca aaagtcacgg atggaggaca cataatccct cccgggcatc
     2761 acccgttcaa aggagtgagc gataaggggt tccgcttctt tcaacccccg tctttcaaac
     2821 ccgttcttga tgagcggata agcgatgtac tcggtggtcc cgggcgggac cgtggtttcg
     2881 atgagcacta ggcaatccgg ctgaattttc tccccgatga ttcgtaagct ctcttccagc
     2941 gccttgatgt cggcatatcc atcctctacg ttgcccaggc tctccttttg atagtcgcac
     3001 tgcacgtcca cgaccaccac atccgccagg gaaagggcgt cgtgggtaaa agtggccatg
     3061 agggtcttct tctccaggac gcagcgccgg atcatggggg ccacctcggg gtcttcggct
     3121 tccacggggg ccatcccttc gtttaggtag gggatcttcc agtaggaccg ggcggagggc
     3181 cgctgcatcc cgattacaaa cttcgaaggc ctgccgtttg acgggttgac gctatccgcg
     3241 acgacgccgg ccataaccgc cccgacgaat ccgactccca taaccaggac gatctcccgg
     3301 cccaaggctc tttgttcttc cacgaccttc cgcaatcttt caacttcccg gtcgtagtcc
     3361 tccttttggg gtaagggaaa aacccggccg gcagggcata ccgaaacagg gggttcagag
     3421 gcccccaaat ctgttttttc aggcttttga atttccatga tttctgctcc tggaaagtat
     3481 atgaaggaaa attcccgtct tcaacctaag gtaaatgtcg caagcccctc agttgaaaaa
     3541 agatcaaatt cttacccttt ttagattatt ttaataccac ggatcaaggt ctaaagcaag
     3601 gaaaatcaac aggttgtctc gttctcatgt ccttcatttt cagaaggcga ttcaggattt
     3661 ccaggagacg gagaggaaaa atggaaggat atggaaaata agaaccatgc ctgcgggttt
     3721 tggaaaattg ggggggtgcc agaagcaaag ggggtaggat tcaggagata tattccttta
     3781 agcactcttc ccagcgtctc atcttccaac cggcgattat ttcaaacttc gagcagtcca
     3841 gcaccgagtt ggccgggcgt ttggccggac ggcccagctc ggcggaggaa atcggcagga
     3901 ggcgtacgcg agggtctcga ggattcttca aggccaatat cttcccggca aattcaaacc
     3961 aagtgcagga gccggagttg gttacatgga ggacccctct ctcctcccgc ttaagcagca
     4021 gttcaatcgc ccggctgagg tcccgagtgt aggtggggga cccccgctgg tcatttacca
     4081 cacgaatctc ttcctgctgc gaagacaagc gcaaaatcgt atccacaaag tttttcccgt
     4141 gctgcccgta gagccaggcc gtacgaagga tcaaataatt tccccccgtc tcttgaaccc
     4201 ccctttcccc cagcagcttg gattccccgt acacattcaa aggacggggc agaatctctt
     4261 cggtgtaggg aaccggggag gtcccgtcga aaacatagtc ggtgcttaga tggatgagtc
     4321 tggccccggt agccgcacat ccccgagcta cctcgagggc accatgggca ttaaccaaaa
     4381 aagccctctc cctctgcgat tcgcaaccgt ccacatcggt aacggctgca agattgatga
     4441 ccacatccgg tcgaagggag tcgagggcct cacggagctt cccgggtcgg gtaatgtcca
     4501 gctcgtcaag gtctgttccg atgacctcgt gccgaggaga caggatgggc tgcaggtctc
     4561 tccccagcat tccccgcgcc ccgatgatga ggattttcac ggcagccctt ccccataatg
     4621 cttctggtag aattcgcggt aggccccgct cttgatccgc ctccaccatt cttcgcggtc
     4681 caggtaccag cggatggtct gagctagccc ttcttccaaa gaaatggaag gggaccatcc
     4741 gagttcaccc ttcagcttgg aaaattcaat tccataccgc cggtcatggc cgggccggtc
     4801 agccacatag cgcagcaaag tcttcggttt ttttaagatg cggagaattt cttcaacgat
     4861 ctccaaattg cttctttccc cgttcccccc gatattgtag acctccccgc ccctgccctt
     4921 ttccagaacc agaagaatgg cgcggcagtg gtcctccacg tgaatccaat cccggatgtt
     4981 caagccgtct ccgtatatgg gcagggtttt cccctcgagg gcgttggtga tcatcagcgg
     5041 gatcagcttt tcgggaaatt gataagggcc gtagttattg gagcaacgag tgatcaccgc
     5101 cgcgaatcca tagctcttga aataagaccg gacgagtagg tcggcgctcg ctttgctggc
     5161 cgcgtaggga ctattggggg cgaggggatg atcttcccgg aagggaggtt ctccggagga
     5221 gagggacccg taaacctcat cagtggagat ttgcacgaag cggcgcacct tgtgacgaag
     5281 ggccgcctgg agcaggacct gaacccccag aacattggta cgcaggaaga ggtcgggttc
     5341 gtagaggctt cggtccacgt gggattcggc cgcaaagttg acgatcgcct ccaccccttc
     5401 gtgaactagg ctttctacca gttttccatc gccgatatcc ccccggacaa atcgatagcc
     5461 ctccaaagag gacaaatcgg acaggttttc caggttcccg gcgtaagtca gtttgtccag
     5521 gttgacgacc tgatattttc cgcgtttctc cagacctaag aggaggcgga tgaagttaga
     5581 gccgataaaa cccgctccgc cggtaaccag taccttcatc ttctttcctt tttcaagttg
     5641 taagttggga gttgggagat gggagttgta gaaggacaaa ccgtctcagg gttcatcccg
     5701 cttggtgttt ttccttttca aactcccaac tctcaactcc cacctcccag tatgggtcac
     5761 ccatccttcc ttgaccaatc ataggggatc tctcccccgt ggggaggcag ccggtattca
     5821 tccgggctct gggagtcata gacttcggtg gggcagttaa ctacgatggc ttcctcctcg
     5881 ctgatgcact taaacccgtg gcggacaaag gggggaatct ggagaaggat ggggttgtgc
     5941 tcccccatga agaattcgtt gatttcccgg aaggtgggcg acttctcccg gccgtcatac
     6001 aggaccacct tcatcattcc cttcactacc acgaaatgat cgaactgctt tttatgataa
     6061 tgccaggcct tcaccgtccc gggataggcg gtggtcatgt agacctgccc gaaacggatg
     6121 aaaattgggt catcggcccg gagcatctcc attaaacgtc cccgctcatc ggaaatgact
     6181 ttcagcctct ttatctggac cccttcaatc atatccacac cgtaagttga gagttgagag
     6241 ttgggagttg ggaattggga gttgggaatt gaaactcctt aattcttgct ctttaatgat
     6301 ttaatgaggc cggttatgat tttgtctaca tctaccattt tctcttctac catagcaatt
     6361 gatccctttt caatataacc cagccttcgg gcgatttcga tataggtatc cacttcgctc
     6421 agggatcccc tggcgatata aaggaattga acgaattctc tccgggtctg ccttgccgca
     6481 ccttcggcca cattccccgg aatagatatc actgcccttc ttaattggct cgtgagccca
     6541 aagacttcat ttcgggggaa ttgcctcgtt agctcataaa ccagcgttac caaagtaacc
     6601 gactctttcc aggcgtcaag cctcttgtgt ggcttctcca taatattcat ctgctctgcc
     6661 gtgtagcgat acctcggcct ttacaactct caactctcaa ctcccaactc tcaactcccg
     6721 cattatagtt tattcgcccc tgtttcggcc accagtctgc tcgctcggag cagggattcg
     6781 aaggtgccgg cgtcggtcca ccatccctcc agaacgtcgt aggtcagggt tccgcggcgc
     6841 aggtattcgt tgttcacgtc ggtaatttcc atctccccgc ggtccgaggg tttcagggtt
     6901 ttgatgatcc gaaaaacctc ggggtcgtac atataaatcc cgatcaccgc gtaattggag
     6961 ggaggatctt ggggtttttc ttcgatacgg aggatccggc cgtcccgaac ctccgggaca
     7021 ccgaagcgat gggcatcggg cacttctttg aggagaatct tggccccccg ctcctgtttc
     7081 tcgaaagccc gaacggcatg gacgatattc ttctcgatga tattgtcccc caggattacg
     7141 cagatctttt ccccctcgga gaagtactct gccaggtcca aggccgcggc aatccccccc
     7201 tccccttcct ggtaggtgta attcacatgc tggagcccga attctttgcc attcccgaga
     7261 agctttagaa agtctccggc atgattaccc ccggtgacaa tcaggatatc ccggatcccg
     7321 gaattgacca gagcctgcaa tgggtaatag atcatgggct tgtcgaagac gggcaagagg
     7381 tgtttgttag tgattttcgt caaagggtag agccgggttc ccaatccacc ggccagaatc
     7441 actcccttca tgaaatcctc ctcgggaatg gtgcgcatga gttcatctca attctccttc
     7501 tccaatttcc cctgaaaata ggggatcgtc ttgccaatac cctctttcaa ttccaccttt
     7561 ggattccatc cgagaaccgc ctgggctctg gaaatgtccg gcttacgcac gcggggatcg
     7621 tcggtcggta gggattgata gatgatccgg gatttgcttc ccgttagctg caaaatcacc
     7681 tgagcgaaat cctggatgga atactcttgg ggattcccca ggttgaccgg ttcaatttcc
     7741 tgggaaaaga gaaggcggat cattccttcc acctggtcct cgatgtagca aaaactgcgg
     7801 gtttggaatc catccccgta aatcgtcatg tcctgccccc ggagtgcctg acagatgaaa
     7861 ttggggacca cccgtccgtc attcaaccgc atgcgggggc cgtaggtgtt gaagatccgg
     7921 gcgatgcgag tgtcgacccc gtggtaccgg tggtaggcca tggtcatggc ctcggcaaaa
     7981 cgcttggctt catcgtatac cccgcggggt ccgatggggt tcacgttccc ccagtagtcc
     8041 tccttctgcg ggtggatcaa agggtcccca tacacctcgg aagtggaggc gagaaggaat
     8101 atcgctttct tttccttggc caggcccagg gccttgtggg tccccaagga cccgaccttt
     8161 aaagtctgga tggggtattg gaggtaatcc accgggctgg ccggggaggc aaagtgcagg
     8221 atggcgtcaa ttttcccttc cacaaagatg taattggtca cgtcgtgttt gatgaaagag
     8281 aacttcgact gaccggccag gtgggcgata ttgtcggtgc ttccggtaat gaggttatcc
     8341 aggcaaatta cctcgtgccc ctcctgcagg aaacggtcgc aaaggtggct tccgatgaac
     8401 cccgctcccc cggtaaccag gaccctcatt gccgccttcc catcccttca tagcggaacc
     8461 ccagcgatcg cagctgcatg gagtccagga aatttcgccc gtcgaagatg tagggatgaa
     8521 tcatgacttg acggatcttt ccccagtcca gctggcgaaa ctcggtccac tccgtggcca
     8581 aaatcagggc ttcggttccc tctgccgctt cgaaggcgct ctgggcgtaa aaaatatccg
     8641 ggaactcctc gcgggtcttc tccgccgcct tcgggtcgta agcccggacc ttcacccctt
     8701 ctctcagtag ccggcggatc aattccaggg ctggggcaaa ccgggtgtcg tcggtctggg
     8761 gtttgaaggc cagacccagg actccgatgg tcttcccctg caggacccag agctccctcc
     8821 tcagcttttc cagggctact tcgatgcggc ccttgttaat cttctccact tcccgtagga
     8881 gcgagaagtc atacccattc ttttcggcga tgcggacaaa ggcctgaagg tctttgggaa
     8941 aacaaaaacc cccgaagccc aggccggccc gcaagaaatc cttcccgatg cgcgcgtcca
     9001 gccccattcc ctgagccact ttatccacat ccgcaccgac cttctcgcag aggtcggcga
     9061 tggcgttgat aaaggagatc ttggcagcca ggaaggagtt tgaggcatgt ttgatgagtt
     9121 cggcgctctt cacgtccgtg accagaaagg caggcaggga ggatggagga cagggagaat
     9181 ggactggaca ggaaaaattt ctctccagaa tcggctggta gagttcgcgc agggtctttc
     9241 ccgcccgctc cttgtccacg cccacaacaa tccggtcggg atggaaaaaa tcattcactg
     9301 ccgaaccttc ccggagaaac tcgggattgg aggccacgtc aaagaggtcc tttttccccc
     9361 ggcagtacat ctgcagggtc cggagtatgt actctccggt ttgcacaggc acggtgcttt
     9421 tctcaatgac cagtttgtag gacttggagc gggaggcgat ttcccggacc accttttcca
     9481 cggcggaaag atcggcctcc ccattctcca ggggaggagt tcccacgcag atgaagatcc
     9541 cttctgcgtc ctggaaggcc atgccgaagt cggtggtaaa agagagcctt cccttttccc
     9601 ggttgtcccg gattaagtcc tccagatgag gttcaaagat cggcatcttc cctgcatcca
     9661 gggtttgaat cttgctttca tcattatcca ggcaggtgac ccggtgaccg atctccgcta
     9721 aacaggttcc ggttacgagt cccacatacc cggtcccggt tacgcatagg ttcatggatt
     9781 ccattccgat cttttggcta tgcccaaact ggcccctgat aaaagccaga attgctatgc
     9841 agaatcatcg acgaacaaat catttctccc ctcctctgac aatgcttcag cattttatac
     9901 aatagaatac cgaaaaataa aaggccccca atccgggttg gatgacgttg acgagaaagg
     9961 ggttgtgttc atgccagagg agcacccctg ggtaagagga gaatcttccc tccgtccgct
    10021 cagattcctc ttttttggaa ccaatgccac gcgaccgcca agccttcggc aaaggggact
    10081 ctgggttcga acccgaggtc cctctgggcc aaccgagtgg aggaaaggga atggcggatt
    10141 tctccgctcc gggccgcttc gtactgggct ggaatttttt tcccggaaat cgcctgcaac
    10201 cgggagagta agtgattcac cgagatccct tggccgcagg caatgttgaa aaccttcccc
    10261 gacactccct cggcttccat ggccaggagg tttgcctgaa ccacattctc cacaaaggtg
    10321 aaatcccggc tctggtttcc gtctccatag atcactggct gggccccgtg gaggagtgcg
    10381 tccatgaagc ggggaatgac cgccgcgtag atggaattgg ggtcctgccg gggcccgaac
    10441 acgttgaagt agcgtagcga aaccgtttcc aacccataga tgcaagcgaa gatccgacag
    10501 tagagttcac ccatgagctt ggtggctgca tagggagatt ctggatgaag gggatcggct
    10561 tcctccttgg ggacggcctc ctccgggttc gagagaaggt tgccgaacac ggaggaggag
    10621 gaggcataga gaaagcgctt caccccggct tcctttgccg cccaaagcat gttcatagtt
    10681 cctgtagcgt tgacccggtg ggtggtgagt gggtcttcca ccgatctttg caccgaaccg
    10741 agggcggcct catggaaaac gcaggagacg ccctcgcatg ccttccggca ggtctcctca
    10801 tcggagatgt cccccctgag aaattccgct ttttccccca gggagatgag aggggttctt
    10861 cccggcccgg catccaaggg cgggacgcgg aacccggtgg cttcctccag gttctccatc
    10921 ttccccgtgc taagatcgtc caggatcctg accttctggc cccggtgtaa caaggcttcc
    10981 accagatgag acccgatgaa accggctcct cctgtgacca atatcataga acctccttcc
    11041 ttccttcgct caagattccg atcatggttt tataaacttc atccacggat atcaattccg
    11101 actctttgcc ccggcgaagg gcaatatttc catctcccca gggagcccag agccgagggt
    11161 cggttttccc gaagaggccc acagtgggag ttcccaccgc agtcgaaaag tgcatggctc
    11221 ctccatcggg gctgacgaag aggcgacagc gctgttggat ggccccgagg ctcttgaaat
    11281 tcggagtagg gtataggaat accccggccc caacccttcg ctggacctcc ttggcccttc
    11341 cctcgtcgcc cgggccccag gttaggatca cggttttccc tttttcctga atgatacgaa
    11401 gagcgagttc ggaaaatttt tccgggcccc agccgttggc cggaagccga ctgctcagat
    11461 ggaagccgat gagccgttca ggatcgatct cgttcccctt taaaaaggca tccacccgat
    11521 ctttctcttc ttgtgggatg aacaccttga gggatttctc agatggctgg actccaattc
    11581 tttggacgag aaagagggcc ttttccacct catgttgctc tggcggcttg ggtactctta
    11641 ggttatagaa aaaagaaaat ttgtggttct tccccacccc cggatcatat cccagccgga
    11701 acggggctcc cgtaaagtaa actatccagg cctccgacca ggaaaaggaa gaccgcattc
    11761 cgatgaccag atcgaacccc ttccggcgca ggtcccactc cacctgcaat tgcttgaaga
    11821 gggagaccat atggtttcgg tccggccggt gtttcgcttt ttcatagact aagacctcgt
    11881 ccaagtcagg gtttccggtt acggcgtcgc ggctgtaggc caccaccaga gcagccaagt
    11941 aggcccgcgg aaaggccctt cggagggcac ggatggccgg ggtggaacag aggacgtctc
    12001 ctacattgtc gttgcgaacc accaggatgc gctggacttg cccagggtca aaagttccct
    12061 tatccagatt ccatttccgg tggatcagca aatgaaaaag cttttctacc cagaagctca
    12121 agattcctcc ctgggcaggg actggataaa atgcgagatc ttctgtgctc tcttcgccca
    12181 ggtgaattcc tggacgtcct cataggcttt ttgcccaagg ccttcagcca gggagcgatc
    12241 ctcaagcaat ctccggatgc cttcccccaa ggcccgcggg tcattgggct ggaccaggag
    12301 ggcgttgacc ccggaggaca agacctcgcg ggtcgaaggc aggtcagagg ccacgatggg
    12361 aatccgggcg gccatgtatt cgaagagctt caggggagag gtgaaggaag cggagatgag
    12421 gtcctgggtc aggggcaaaa ccgccacaga tgaatctgcc aggtggcgtt tgacctcccg
    12481 cggaggcagt tgaccgagga aaaaaacccg gtcttgcacg cccagccggg cggccttctc
    12541 cctcagggct tgaattcgct cctcacttcc cccgatcaga tggagttccc cgatgggcaa
    12601 atattgcatg gcctccacta gcgtcccagt tcccttccag ggatagagtt ggcccacata
    12661 aatgatcttt tgatttcgcg gtttccgggc gattccgcca aaaaaatcca gattgactcc
    12721 atcgggtacc acagccatgg gaatctgaag ggagaagact tccttcattt gtcgcgccag
    12781 accccgggta atggaaatga tcccatccag gcgggggtaa atcttgaact cctctctttt
    12841 gatcttttcc atcttgccat ggtgatccgt ggtaagaaag aagatttcat gagcctcaaa
    12901 aagcatggga aggtgcagat acctcttgaa cctaaggaag aacgagactg tgttcaggtg
    12961 ccgaataaag atggcatcat aagccctttg ccgcaagagt tcccgaacct tcaccaggca
    13021 ggcccagagg aaaactccgt cccaggatat cctcagaaac cggtcttctt ctctccgcaa
    13081 catgggaagg agatggatac gcaggttttc gtggggccaa agcccgtagt gggacagccc
    13141 ttccgatagg acgtctaccc gattcctccc gatgatcaga tcgacctcgc aattccggcg
    13201 cgccagggca tggaccgtct ggaagacctg gtggaaacgg gcccgccgaa aatcgacccc
    13261 ttcgtggatc gggtagataa tccgcagggg attcc
//
LOCUS       DASPRR010000256        13392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_546739, whole genome shotgun sequence.
ACCESSION   DASPRR010000256 DASPRR010000000
VERSION     DASPRR010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13392)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13392
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546739"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VEL68_10020"
     CDS             complement(<1..1297)
                     /locus_tag="VEL68_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ36343.1"
                     /translation="MSLLVRSGKALKGQGNALERADFLIIGDRISEIGLNLPAPDGTK
                     VIDATGHLILPGLINAHTHAHNNLMKGTADNWTLEDLLNHGGALNGNRTSEDHYLSAA
                     LGAVEMLRTGCSAAYDLFMGIPLPTIEVFEAVVQAYQDVGLRAVVAPAVADIVFYQTV
                     PFFMELLPPDLVARVQEMKAAPTDKMLQLSEEGIRRWNGAAGGRIHVGIAPTIPGQCT
                     DEFLKGCLRLTREYGVGVHTHLVETKIQAIFGHRRWGKSVPAHLAEIGLLNPKFVGAH
                     GVWLTDEDIKILADHGSNVAHNPASNLKLGSGTAPVRELLDAGVNVGLGCDGSMSSDN
                     QDLFESMRFAAMVGKIRFPHQTQRWVGSRDTWKMVTMGSARVLDWADEIGELAPGKKA
                     DLTLLREESPYLKPLNDILNALSYAETGGDVAMVIVDGRV"
     gene            complement(1326..2384)
                     /locus_tag="VEL68_10025"
     CDS             complement(1326..2384)
                     /locus_tag="VEL68_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36344.1"
                     /translation="MLRKKIGRNHSGGKKNLFQFFVISLGIAWVFFPPPNVEAQALKP
                     FTFILDFLPYGEYTPYFTALDKGWYKEEGLDVKILRGAGSGDTVKRIAVGQGDAGSAD
                     FSALTAARANEDVKVKAIGAYFRRPPHTIFVRTDTGINSPKDLEGKTIAITPGNSHQI
                     LFPLFAQLAGFKADSVKWITMDGAAMGPALITGRVDGAPLFANHEARLQKQAREQGKG
                     VKRLSYADYGLDMYSLVIIAREETIQKNADSFKRFLRATVRGMKYAFAPQNIEEGAKI
                     VVKINPEVDLEAAIGAAQIASRYSFTDEITSGKVAVGQFEPARVEKSIGIYTQYLNLK
                     KKVSPEEVYTNDLLPEKK"
     gene            complement(2828..3223)
                     /locus_tag="VEL68_10030"
     CDS             complement(2828..3223)
                     /locus_tag="VEL68_10030"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HXZ36345.1"
                     /translation="MKRKLTNWVAWYMVTVMFLFGITPKVEAGFSPSEVIGQSQMDRS
                     MDVEKVRKFLETKMIHERLHAFGFSQEEIQARLNLLTDDQIHQVALKLDELKVAGDGG
                     EAVIIVLLIAAIVLLVIYLLGYRVVLKKE"
     gene            3615..6899
                     /locus_tag="VEL68_10035"
     CDS             3615..6899
                     /locus_tag="VEL68_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HXZ36346.1"
                     /translation="MGNVIRPVLRRVFPRERLFRLIDRMRDRSVIWVSGPPGCGKTTL
                     VNSYLEARKVPCLWYQVEEADADAATFFYYLGQAGKRAGVIGQEPLPLLTPEYLQGLP
                     TFTLRYFENLYSRLSPPGVIVFDNYHRVPAGSSVHEIILNALSLIPEGLHFFFISRSD
                     PPSDLIRIQADNLMEILGWDKLRLTVEEAGEIIRLRSKKMPARREIIGLHRASDGWVA
                     GLMLMLGRSRVEDLEIKLAGDFIPQEVFDYFAAEIFDRTGEEVKQFLLKTSFIPQMTV
                     KMAEELTGLSSASRILSGLSRDNYFTDEHLQPEPLYQYHPLLRRFLSSKAKDFFSAEE
                     VVQIQRKAGQLLEGSGRLEDAAKLYREGKDWGNFVRFVIGQAHSLLKQGRLRTLQEWI
                     RSIPKEMTDNHPSLLLWLGTCQMSFEPEESLESFKKAFRLFQDQGDVSGAYLAWADVV
                     VLLTYEQDDWALLAPWVEWLTEQTRISPHFPSPKIEAKVAVSLAGALLMLRPQHPDLK
                     KWMERSLELSREIRDAHLNMRALITVAHWCQWIGDMARCSLLIEQLERIAQPAFTSPL
                     LMIVKKWLEACACNLGPAADFDSALRLAREGMKTALETGVSFWNSMLHAQAVVACLNQ
                     GDLANAGKFLQEMHSNLKSKQRLLSCQYHYLSAWYYLLLGDPSRALLASRTAVNLAAE
                     IGSAFQEILCRLEMAQILNSAGEKKVAVLQLNLAGELTQQCGSLTLEYSYLMAKAQFS
                     LDEDEEPAGLEFLRRAMALGKSQGYVSMYCWWEPSVMARLCARALGEGIEVEYARNLI
                     RKRNLHPDPLGQTLENWPWPLKVFTLGQFKLERDGKRLVFQGKAPQKALLMLKALIAL
                     GVRDVRQEQIIDLLWPESEGDTAHIAFKTLLSRLRQLLGVKDGGITLQGGKVGFNPEI
                     CWVDTWVFERIFSRAREAWEEKRSSDRFGKAGRLTEAALAIYKGHFLQCDEGYDWTSP
                     HRERLQRKFLFLINKLGEHLLETKNWKEAVEHYEKAIEIDPLPEAFHQNLMIGYLELG
                     QGDEGIAVYRRFRKTIAGGTGITPSRKTEAIYQSLLDSQTSPQIGRLAERAALKEDDP
                     KAQPPG"
     gene            7370..8290
                     /gene="nrfD"
                     /locus_tag="VEL68_10040"
     CDS             7370..8290
                     /gene="nrfD"
                     /locus_tag="VEL68_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXZ36347.1"
                     /translation="METHIPSAVLYNVPHVIPIGYFIAVYFYITGLHMGFYTTSVVAT
                     LLGREEWKPIGKIGAVGALIVLAVAPIFLLLDLTQPLRFWHLFSYINWRSAITWGTFF
                     LTAYPPIGVIYAICVLRGNYRAAKIWGLCGFPIAVSVHGYTGFILALAKGRAFWNNPL
                     NPILFLVSAMVSGLALMIIILHIRKRILHLEGEQAATDSRITSNLVMMMFIFLIIEIF
                     LMASDLAVLAHSRKDEYEMFHMLTEGNFAPLFLGVELTLGGLIPLILLSIKRIRLQPL
                     GQCVACGFILCGILAMRIIIVIGGQSVRLF"
     gene            8452..8694
                     /locus_tag="VEL68_10045"
     CDS             8452..8694
                     /locus_tag="VEL68_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36348.1"
                     /translation="MNQTNQIFYKDFIVWASPMQLAESGEWTIEVNIRRKDGEAVNRF
                     FSKNTFKTEREAVYHSEKYGKLIIDGKISGCSICKF"
     gene            complement(8914..9906)
                     /locus_tag="VEL68_10050"
     CDS             complement(8914..9906)
                     /locus_tag="VEL68_10050"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ36349.1"
                     /translation="MKPLLHAELVNSPLEDPALFVEILWEHRALLFDLGEIGGYRPAK
                     LLKISHAFVSHTHIDHFVGFDTLIRLMLNREKALKIFGPPGFLANVQGKLAGYTWNLT
                     EGYPFAVIAAEVHPDRMISETFRSQYRFIPEGEKEEPFSRILEEDSHLKISATHLDHF
                     IPSLAFILEERFHINVHKERLAERGLSIGPWLKEMKEALWKGETGDFRLQVPLESGGD
                     VEEKEFSLEDLKETVTLTPGQKIAYVADCRFTGENVQRIIQLAAGADLFFCEAGFLDR
                     DRERAEERGHLTARQAGDLARQAKVKKLQIFHFSPKYEKEADLLYKEAEEAFRG"
     gene            10113..11381
                     /locus_tag="VEL68_10055"
     CDS             10113..11381
                     /locus_tag="VEL68_10055"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36350.1"
                     /translation="MNAGGRRFHYGWIILLVGFIGVMGALGFGRFAYTPILPSMKEGL
                     SLTYTQMGWIGSGNFTGYLVFSLVGGYLATRIGPRLVISIALILVAFSLFLTGISDTF
                     EFAVAMRTLTGAGSAASNVPIMALASAWFATSRRGMATGILVSGSSIALLLLGPLIPF
                     ILQNSPRAGWRVCWYVLGGITLLIALLNYLFLRNHPEEKNLLPFGKNAYPPSVSSSPS
                     SFSMKRFYTSRPLWHLAGIFFTFGFSYIVYMQYFSAYLINEARVGAERAGNYLMLLGI
                     LSIFCGPFWGTVSDFIGRKYGLALVFLTQGISYLFFGLLKSDAGYLLSTILFGLTAWS
                     VPSIIAAAVGDTVGPRLTPAALGFTILIMGMGQALAPPIGGRIADLTGSFTSAFLLSA
                     VVAFAGMTASLLLQKSTQKFDFQLDADKRG"
     gene            complement(11456..12700)
                     /locus_tag="VEL68_10060"
     CDS             complement(11456..12700)
                     /locus_tag="VEL68_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36351.1"
                     /translation="MILIYGIRHSFAVFFTPILEEFTWSRGNISIMMSLNIFIYGFLA
                     PVTGILADRWRPRILMPLGIAVLGVATASCAWARELWHFYILFGILMPLGSALAGWPI
                     IAPAIMNWFVKKRGLVMGIGQMGGGLSFVYTIFVKYSILQVGWRPTFLVMAGMLVSVL
                     FPLYLLFFYYRPQDKDLKPYGSGEMGLASEGEKKKAVGRIPTARDWTLSQILCSSQLW
                     LLVLSYALYWGVGGYLVLAHQVRFAEDSGYSSMFSVSIFALLGVTLFLGQSCGFLSDR
                     MGRERTATLAASLSIIALVALISVRDNSQPWLLYFYSICFGLGGGLFTPTIFAGSADI
                     FYGRHFGAVPGLLLTGMGVGAVFGPWLGGYIYDVTGSYLPAFFLCIVCIAFSCLTFWI
                     AAPRKGIKPKPTREIKSAQRVA"
     gene            13058..>13392
                     /locus_tag="VEL68_10065"
     CDS             13058..>13392
                     /locus_tag="VEL68_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36352.1"
                     /translation="MAKHNQGREPKGKLTLFLGPFQPELEEAFSLWVRERKEKDPLAP
                     LVVLVGSNLLGLYLRRLLAQRGLNHINLRFLTFIDLARTLAAEPLDRKGLRPLPRYGD
                     LVLISALAE"
BASE COUNT         3093 a         3581 c         3426 g         3292 t
ORIGIN      
        1 tgacccgtcc atctacgatc accatggcaa cgtctccccc ggtttcggcg taggagaggg
       61 cgttcagaat atcattcagc ggttttagat aaggagattc ctcccttaac aaggttaaat
      121 cggctttctt gccgggtgcc aattcaccga tttcatccgc ccaatcaaga acgcgggcgc
      181 ttcccatggt taccatcttc catgtatccc gggaacccac ccacctctgc gtctggtgcg
      241 gaaaccggat ttttccgacc atcgcggcaa accgcatcga ttcaaaaagg tcttgattgt
      301 cggagctcat ggacccatca catcccagcc ctacattgac tccggcatcc aacaactccc
      361 ggaccggggc ggttccactg cccagtttca ggttgcttgc cggattatgg gccacattcg
      421 agccgtgatc cgccagaatc ttgatgtcct catccgtcag ccaaactcca tgggccccga
      481 caaacttggg atttaacagg ccgatttcgg ccaaatgggc cgggacggat tttccccatc
      541 gcctgtggcc gaatatggct tggatcttgg tttccaccaa gtgggtgtgg actcccacgc
      601 catactcccg ggttaagcgc aggcagccct ttaggaattc atcggtgcac tgcccgggga
      661 tagttggggc gattcccacg tgaattcgtc cacccgccgc cccgttccac cggcggatgc
      721 cctcctcgct caattgaagc attttatccg ttggagccgc cttcatttcc tgcacccgcg
      781 ccaccaggtc cggcgggaga agttccatga aaaagggaac ggtttgataa aagacgatat
      841 cggccacagc cggggccacc acagcccgca ggcccacgtc ctgataggcc tgaacgaccg
      901 cctcaaaaac ctcaatcgtc ggaagcggga tgcccatgaa aaggtcgtag gccgcggaac
      961 atccggtcct gagcatttcc accgccccca gggcggcgga aagataatga tcttcagacg
     1021 tgcggttgcc gttgagggcc cccccatggt tcaggaggtc ttccaaagtc caattatccg
     1081 cggttccctt catgaggtta ttgtgggcat gggtatgtgc gttgatcagc ccgggtagaa
     1141 tcaggtggcc ggttgcatcg atgaccttgg ttccgtcggg tgccggcaga ttcaggccga
     1201 tctccgatat gcgatccccg ataatgagaa aatcggcccg ctccagggcg ttcccctgcc
     1261 ctttgagagc ttttccactc ctaaccagca aagacatgaa gacgtttcct ttccctcagg
     1321 ttttcctatt tcttctctgg caaaagatca ttggtgtaga cctcttccgg cgagactttc
     1381 ttcttcaggt tcagatactg cgtgtaaatc ccgatggatt tctcgaccct tgccggctca
     1441 aactggccca cggcgacttt tcccgaggta atctcatcgg tgaaggaata ccggctggcg
     1501 atttgggcgg ctcctattgc cgcttccaag tcaacctccg gattgatctt gaccacgatc
     1561 ttcgccccct cttcaatatt ctggggagca aaggcgtact tcatgcctcg caccgtggct
     1621 ctcaggaacc ttttgaaaga atcagcgttc ttttggatcg tttcctcccg ggcgatgatt
     1681 acgagggagt acatgtccag tccgtaatcg gcgtaggaca aacgtttgac ccccttcccc
     1741 tgctcccggg cctgtttttg caaccgggct tcatggttcg caaacagggg ggcgccatcg
     1801 acccggccag tgatcagggc tggccccatg gcagccccgt ccatggtaat ccatttcacc
     1861 gaatccgcct tgaatccggc taactgggca aataacggga aaagaatttg gtggctgttc
     1921 cctggggtaa tggcaatcgt cttcccttca aggtctttgg gggaattgat tcccgtgtca
     1981 gtccgaacga aaatcgtatg aggaggacgg cgaaagtatg ccccaatggc tttcacttta
     2041 acatcttcat tcgcccgagc cgctgtaagg gcgctgaaat cagcgcttcc agcatctcct
     2101 tgcccgacag caattcgctt gaccgtatcc ccggatcccg caccccgcag aatcttaaca
     2161 tccaggcctt cttccttgta ccatcctttg tccagggcgg taaaatacgg cgtatattca
     2221 ccatagggta aaaagtccaa aataaaggtg aacggtttca aggcttgggc ttcaacgttt
     2281 ggaggcggga agaaaaccca ggcgatcccc aagctgatga cgaaaaattg gaacagattt
     2341 ttctttccgc cggaatgatt cctgcctatt tttttcctta acatctgact tcctcctttc
     2401 cggaacccaa aatggaatgg aataccttac aagcaattga tccttatctt agcagcaact
     2461 ttaaaaactc tgcgaaatat ttcatcaaca tggctaaggg caagagcaag gcatgattcg
     2521 gaccacagca taggcttcgt cctaacctaa agagaatgag aatggggtca ggcttaacta
     2581 attaactatt tcttcttgag cgaagttgtc tgggggtcgg accgagtaaa cattttgatt
     2641 ttcataaaga tgcggcccaa aagaaggcgt ctttctccct tagaaaatca ttttcggggt
     2701 aagaaatagc cttgtaagcg gctctcttgc aactaataat aaaaaggcct ggcccttgat
     2761 ccgtcccccc acctaacctt tccccgatat taagctgcaa agggaaagtt ggatttatta
     2821 aaaccggcta ctccttttta agaaccaccc tatatccgag aagatagatg acaaggagaa
     2881 caatggcagc gatcaaaaga acaataatga ctgcttcccc accatcgcct gccactttca
     2941 gttcatcaag ctttaaagcc acctgatgaa tctggtcatc ggtgagcaga ttcaacctcg
     3001 cctgaatttc ttcctggctg aagccaaagg catgaagtct ttcgtggatc attttggtct
     3061 ccaggaactt ccggaccttc tccacatcca tggacctatc catctgcgat tggccaatga
     3121 cctcggaggg cgaaaagcct gcctcaacct tcggggtaat tccaaataag aacatcaccg
     3181 tgaccatgta ccatgcgacc caattcgtaa gtttcctctt catttttctt cccccttctt
     3241 acttatgaaa aggtttgaag aaatttagag atatgatttt tttactacaa tttctggcgg
     3301 aagtaaaggc cgtttcagaa ggaatataag gctgatgggc ctttgaaagg gattttatgg
     3361 aagagtccag gccaaaagcc tgggttcttt atgttacgcg gttacgaaat gggtgcagat
     3421 gggatagata tggactgcat ttcattgtcg gcttggcttt ttttgtaaca acatggattt
     3481 atgtggattt ttttaattta tccaaataat tcttgacaaa cctttaaata ggaatattat
     3541 tctccatatc atttcctcct gaagccagtc aaacaacaac aaaaccttca accttctgag
     3601 gtataagagg aatcatgggt aacgtaatcc gcccagtcct cagacgggtc tttccaagag
     3661 aaagattgtt tcgattgatc gaccgcatgc gagaccgatc ggtgatctgg gtctccggcc
     3721 ctccgggatg cggcaagaca accttggtaa acagttatct tgaggcccgc aaggtcccct
     3781 gcctctggta ccaggtcgag gaagccgatg cggatgcggc aacttttttt tactacctgg
     3841 gccaggccgg aaaaagggcg ggggttatag ggcaagaacc tctccctctc cttactccgg
     3901 aatacctgca aggattgcca acatttaccc ttagatattt tgagaacctt tacagccggc
     3961 tgagccctcc cggggtgatt gtttttgaca actatcatag ggtgcccgcc gggtcctctg
     4021 tccatgagat cattctgaat gccctttctc ttatccctga aggcctccat ttctttttca
     4081 tcagtcgaag tgatccgccc tccgatttga tccgaattca agccgataat ctgatggaaa
     4141 tcctaggatg ggacaaactc cgtctaaccg tcgaggaggc aggcgaaatt atccggctgc
     4201 ggagcaaaaa aatgccggcc agaagagaga tcataggctt gcaccgcgcc tccgacggat
     4261 gggttgccgg cctgatgctg atgctggggc ggagccgggt cgaggatttg gagatcaagc
     4321 tggccggaga tttcatcccc caggaggttt tcgactattt cgccgcggaa atcttcgacc
     4381 ggaccgggga agaagtgaaa caatttctcc ttaagacatc ctttatcccc cagatgaccg
     4441 tcaagatggc cgaagagctc accggcctct cctcggcaag ccgcatcctg tccggcttat
     4501 cccgggataa ttattttacc gatgagcacc tgcagcctga gcctttgtac caataccatc
     4561 ccctgctgag aaggtttctt tcctccaagg ccaaagattt tttttctgcc gaggaggtgg
     4621 tccaaatcca gcggaaggcg ggccagcttc tggaagggtc tggccggctt gaagatgcgg
     4681 cgaaacttta ccgtgagggg aaggactggg gcaatttcgt ccgttttgtc atcggccaag
     4741 ctcattctct gctgaaacag gggaggttga ggacccttca ggaatggatt cgaagcatac
     4801 ctaaggagat gacggataat catccttcct tgctcctctg gttgggtacg tgccaaatgt
     4861 cgtttgaacc tgaagagagt ctggagtcct ttaagaaggc cttccgtttg ttccaggatc
     4921 agggggatgt ttccggggcc tatctggcct gggcagatgt ggtggtcctg ctcacctatg
     4981 agcaggatga ttgggcgctc ctggctccct gggttgaatg gcttaccgag cagacccgca
     5041 tcagtccgca tttcccttct cccaaaattg aggccaaagt ggcggtcagc ctcgcaggag
     5101 cattattgat gctgaggcct cagcaccccg accttaaaaa gtggatggaa cgatctctgg
     5161 aactatcgcg ggaaataagg gatgcgcatc tcaacatgcg cgcgctcatc accgtcgccc
     5221 attggtgcca atggattggg gatatggccc gctgcagcct gctgatcgaa caattggaga
     5281 gaatcgccca acccgctttc acttctccgc tcctaatgat cgtgaagaaa tggttggaag
     5341 catgcgcctg caatctgggt cccgcagcgg attttgactc cgccctgcgg ctggccagag
     5401 aggggatgaa gacagccctc gaaacggggg tctccttttg gaattccatg ctccatgccc
     5461 aggccgtggt ggcctgtttg aaccaagggg acctggccaa tgccgggaaa tttcttcagg
     5521 aaatgcactc caatctgaaa agcaaacagc ggcttctttc ctgccaatac cactacctgt
     5581 cggcatggta ttacctcctt cttggggatc cttcccgcgc gcttttggct tccaggaccg
     5641 ccgtgaacct ggcggcggaa atcggctccg ccttccagga aattctctgc cgcctggaaa
     5701 tggcccaaat attgaattcg gctggcgaaa agaaggttgc ggtccttcaa ctcaatcttg
     5761 ccggcgaatt gacccaacag tgcgggagct tgactttaga gtactcgtac ctgatggcca
     5821 aagcccagtt tagcctggat gaggatgagg aacctgccgg cctggaattc ctgcgcaggg
     5881 cgatggcttt gggcaagagc caggggtatg tgtcgatgta ttgctggtgg gaaccctcgg
     5941 ttatggcgcg tctttgtgcc agggcccttg gtgagggaat cgaggtcgaa tatgcgcgga
     6001 acctgattcg caaacgcaac ctccacccgg accctctcgg tcagacttta gaaaattggc
     6061 cctggccttt aaaggtcttt accctcggtc aattcaaatt ggaaagagac ggcaaacgat
     6121 tggtcttcca gggaaaggcc ccgcagaagg ctttattgat gctgaaggca ttgattgcct
     6181 tgggggttag agatgtccgc caggaacaga tcatcgatct gctctggcct gaaagtgaag
     6241 gagatactgc ccatatagca tttaagactc ttctttcccg cctccgtcag ttgctggggg
     6301 taaaggatgg ggggatcacc ttacaggggg gcaaggttgg ttttaatccc gaaatctgct
     6361 gggtggatac ctgggttttc gagaggattt tttcacgggc aagggaggcc tgggaagaaa
     6421 aacggtcgtc agaccgcttt ggaaaggccg gaaggcttac cgaagcggcg ctggctattt
     6481 ataagggcca cttcctccag tgtgacgagg ggtatgattg gacatctccc caccgagaac
     6541 gtttacaaag aaaatttctt tttttaataa ataagctggg ggaacacttg ctggaaacaa
     6601 aaaattggaa agaggcggtg gaacattatg aaaaagccat cgagatcgac ccccttcccg
     6661 aggcgtttca tcagaatctc atgattggtt accttgagct cggccaaggc gacgagggga
     6721 ttgcagtgta ccggcgtttc cggaagacta ttgccggggg cacgggcata acgccctcgc
     6781 ggaagacgga agcgatttat caatccctcc tagacagcca gacctctccc cagatcgggc
     6841 ggcttgctga aagagctgcc ctaaaggaag atgaccccaa ggcccaaccg ccgggctgaa
     6901 gcgcgccttt ttattcccat ggatggccat accttccggc agaggtggag gcttgaaaat
     6961 atgatgtccg ctcaaagcgg atcggccctg ggtcggccga tgtcggcctt cgcgcgggtg
     7021 cccccaggcc cgtggccatg gagcttgcgc ctggttggat gaaagcagcc atcttttttc
     7081 cccccatgtt ttttcataaa atttgtcctt ctaatttttt ttctctctcc ctaaaaaatt
     7141 tgtagccaat ctgtagccaa tctataacca atctattctc ttactatttc tttttttctg
     7201 cagatatggg taacccatcg gtaacctatt aattacctaa aagtatccat tttataacct
     7261 aattataacc tctcccatgg tagctgattc ttgataatgg cggtggtcct gtcgtcgaat
     7321 tgccctggtt catttaatct gtttaaaaaa agaccaaagg aggaacacca tggaaacgca
     7381 catcccctca gcggtcctgt acaatgtgcc ccacgttatt cccatagggt acttcattgc
     7441 cgtttacttc tacatcaccg gcctgcatat gggcttttac accacatcgg tggtggctac
     7501 tctgctgggg agagaagagt ggaagcccat cggcaagatc ggagccgtag gcgccctgat
     7561 cgtgctcgct gtcgctccca tctttctcct cctggacctt acccagccgc tccggttctg
     7621 gcatctcttc tcttacatta actggcgctc ggccattacc tggggtactt tcttcttgac
     7681 cgcctatccc ccgatcgggg tgatctacgc catctgcgtg ctgaggggca attaccgtgc
     7741 ggccaaaatc tggggcctct gtggtttccc cattgccgtg agcgtccacg gttacaccgg
     7801 ttttatcctg gccttggcga aggggcgggc cttctggaat aaccctttga atcccattct
     7861 cttcctggtg tccgccatgg tttccgggct ggctttgatg atcatcattc tccacatccg
     7921 gaaacgcatc ttacacctgg aaggggagca agcggccacg gattcccgca taaccagtaa
     7981 cctggtcatg atgatgttca tcttcctgat tatcgaaatc tttctcatgg ccagcgacct
     8041 cgccgtgttg gcccactcca gaaaggatga gtatgaaatg tttcatatgc tcaccgaggg
     8101 gaattttgct cctctattcc tgggggtaga gctcactctg ggagggttga ttcctttaat
     8161 cctgctgtcg atcaagcgaa ttaggcttca gcccctgggg caatgcgtgg cttgcggttt
     8221 catcctgtgc ggaattctgg cgatgcgcat tatcatcgta atcggcggcc aaagcgtgag
     8281 gttgttttag gggaagaaca accttcattt ctgatccgta tcaggcttgg tccaaattag
     8341 ggaatcaatc ggaaccagtg accggaatgt atcgggataa gcatcccttt agctaatttg
     8401 gaaagggccg aagaaaaata aattcgggag ggggatctat cggaggtcca tgtgaatcaa
     8461 accaatcaga ttttttataa agattttatt gtttgggcaa gtcctatgca gttggctgag
     8521 tccggagaat ggacaattga ggtcaatatc aggcggaagg atggtgaagc ggtcaacaga
     8581 ttcttttcaa agaatacctt caaaacggaa agggaggcgg tttaccattc tgaaaaatat
     8641 gggaaattaa tcattgatgg aaagatctct ggttgttcca tctgcaaatt ttgaattaca
     8701 ggaaatggac cccctaaggc ccatctcctt ccccgaaagc catgagaggc aggcaatgag
     8761 aagcggggta acctgcccca gatatccaag acttgatgct tctggggttt tgcatcatgc
     8821 aacgggccgg gggcttgaaa gaacaaaaat atcctaatgg aggctggtca ggattttctg
     8881 gattgagtag ccaactcttt tctcttcgaa agttcatcct cggaaggctt cctctgcttc
     8941 cttgtataga aggtcagcct ccttttcgta ctttggagag aagtgaaaga tctgtagttt
     9001 cttcaccttt gcctggcggg ccaggtctcc ggcttggcgg gcggtgaggt gtcctctctc
     9061 ttcggccctt tcgcgatccc tgtccaggaa gcccgcttca cagaaaaata ggtctgcgcc
     9121 agcagccagt tggataatac gctgtacgtt ttctccggtg aagcggcagt ccgccacata
     9181 ggcgatcttc tgaccggggg taagggttac cgtttccttc aagtcttcca gagagaattc
     9241 cttttcctcc acatctccac cgctttcgag ggggacttgc agccgaaaat ctcctgtctc
     9301 ccctttccat agcgcctctt tcatttcctt cagccagggc ccgatggaaa ggcctctctc
     9361 cgcaaggcgc tctttatgca cgttaatgtg gaagcgctcc tccaggataa aggcaaggga
     9421 ggggataaaa tggtccagat gggtggcgct gattttcaga tgggaatcct cttccagaat
     9481 cctgctgaat ggctcctctt tttcaccttc cggaataaag cgatattggc tgcggaatgt
     9541 ctcagaaatc attcgatccg gatggacttc ggcggcgatc actgcaaaag gataaccttc
     9601 ggtcaggttc caggtataac cggcaagttt cccctgcacg ttcgccagga atccaggcgg
     9661 tccaaaaatt ttcagggcct tttcccggtt caacatcagg cggatcaggg tgtcgaagcc
     9721 gacaaagtga tcgatgtggg tgtgagaaac gaaggcgtgg gaaatcttga ggagcttggc
     9781 cggacggtaa cccccgatct cccccagatc aaagagcaaa gcccggtgct cccagagaat
     9841 ctccacaaag agcgccgggt cttctaaagg agaattgacc agttctgcat gaaggagcgg
     9901 tttcaaaagg aatcttcctt actctaggaa tattgaggaa gatcatacgg aagaggaaac
     9961 catccgtcaa tgctcaagat ttcccctctc aggcccatcc ccgaattcct ttaggggtgg
    10021 ttcgcaaacc gcccttactc tggatagatg ataaatgggt cttttatctg aattttcttt
    10081 cgaaaaacct ggagctgtgt tatggaactg gtatgaatgc aggggggagg cgttttcatt
    10141 acggctggat tattctgctg gtgggcttca tcggggtcat gggggcgctg ggattcgggc
    10201 ggtttgccta tacccctatc cttccttcca tgaaagaggg cctgtccctc acctacaccc
    10261 agatgggatg gatcgggtcg ggaaacttca ccggatacct ggttttttcc cttgttggag
    10321 gctatttagc cacccgcatc ggcccccgcc tggtgatctc gatcgccctg attctggtgg
    10381 cctttagcct tttcctgacc gggatttctg atacctttga attcgccgtg gccatgcgta
    10441 ccctgactgg ggcaggaagc gcagcctcca acgttcccat catggccttg gcctccgcct
    10501 ggttcgctac cagccgcagg ggaatggcta ccggaattct ggtaagcggg tcaagcattg
    10561 ctctgcttct tctaggtcct ctgatcccct tcattcttca gaactccccc agggccggct
    10621 ggagagtctg ctggtacgtt ttgggaggaa tcaccctcct cattgctttg ttgaattatc
    10681 tctttctccg caatcacccc gaggagaaga atttgttgcc atttggcaag aatgcttatc
    10741 ctccatccgt atcctcttca ccctcctcct tctccatgaa gaggttttat acctcccgtc
    10801 cgctctggca tctagcgggg atttttttca ccttcggctt ttcttacatc gtttacatgc
    10861 aatatttctc cgcctacctg atcaatgaag ccagggttgg agcagaacgg gcaggtaatt
    10921 acctaatgct tctgggaatc ctgagcatct tctgcggccc tttctggggg accgtctcgg
    10981 atttcatagg gcggaaatac ggcctggcgc tggtctttct gactcaggga atctcctacc
    11041 tttttttcgg ccttctgaaa tctgacgctg gttatctctt atccacgatt ctttttgggc
    11101 tgaccgcctg gtcggtccct tccatcattg ccgcggccgt gggggatacc gtgggcccgc
    11161 ggctcacccc ggcagccctg ggcttcacca tcctcatcat gggcatgggc caggccctgg
    11221 ctcctcccat cggcgggagg atcgccgacc tgaccgggtc tttcacttcc gccttcctgc
    11281 tctccgcggt cgtcgcattc gccggcatga ccgcctctct cctcctccaa aaatccaccc
    11341 aaaaatttga ttttcaattg gacgcggata aacgcggata acaaaatttc cctgggtaaa
    11401 accaggaaca acctaacatt ggggtgttaa cccctccggg ttgaaaaata tgcccttatg
    11461 ctactctctg tgctgacttt atttcacggg tcggtttggg tttgattcct ttgcgaggcg
    11521 cggcaatcca gaaggttagg caggaaaagg ctatgcagac gatgcagagg aaaaaggcag
    11581 gcaaatagct ccccgtcacg tcataaatat atccccccag ccagggaccg aagactgctc
    11641 cgacacccat tccggtaagc agcaggccgg gaacggcgcc aaaatggcga ccataaaata
    11701 tatccgcgga accagcgaaa atggtgggag taaagagccc tccccccaga ccaaaacaga
    11761 tggaataaaa gtacaagagc cagggctgtg aattgtcgcg gacggagatg agagccacga
    11821 gggctatgat ggacaaggag gcggccaatg tggccgttct ctcccttccc atccggtctg
    11881 ataagaaacc gcaggattgg cccaggaata gggttacccc taaaagagcg aagatggaga
    11941 cgctgaacat gctgctatag cccgaatcct ctgcgaatcg aacctgatgc gccagaacca
    12001 agtagccgcc aactccccaa tagagggcat aagacaaaac cagcagccac agctgagagg
    12061 aacagagaat ctgggaaagg gtccaatcac gggcggtggg aattcttccg accgcctttt
    12121 tcttttctcc ctccgaagcc aggcccattt cgccagagcc ataaggtttg aggtctttat
    12181 cctgggggcg ataatagaaa aataggaggt acagggggaa gagaacggaa accagcattc
    12241 ccgccattac cagaaaggtg ggcctccatc caacctgaag aatgctgtat ttcacgaaga
    12301 tcgtgtagac gaaactgagc cctcctccca tctgaccaat gcccatgacc aatccgcgct
    12361 ttttgacgaa ccagttcatg atggcgggag cgatgatggg ccagccggca agagcgctgc
    12421 cgaggggcat gaggattccg aacagaatat agaaatgcca cagctcccgc gcccaggcgc
    12481 aactcgcggt ggccaccccc agaacggcga ttcccaaggg catcaggata cggggtctcc
    12541 acctgtcggc aagaatcccc gtgaccgggg ccagaaaccc gtatataaaa atattcaggg
    12601 acatcatgat ggagatgttg ccccggctcc aggtaaactc ctccaggatg ggggtgaaaa
    12661 aaacagcgaa ggagtggcgg attccataaa tcaggatcat gccaacgata cctgccgaga
    12721 caattttcca tccgtaaaag aggcccggcc ttcggccacc gtgacttttt tctggtccca
    12781 ttcttaagtc atgacctcag gcagagccgg aggattgaaa aacagggaac cgctcaaagc
    12841 ggagatcaat agctgccact taaagctagg ttccctccgt tgcccggccc gggggcaagg
    12901 ggattacgcc cgggccacga ttcaggcgtc cctgctacaa gaaaacaggc cccgtcgcct
    12961 ttgccctctc cccttttttg gaagaagggt aaggaggcat tatgcctttc cattccgatc
    13021 gaaacccgtt atcattcatt atatcggaat taaaaccatg gccaaacata atcaagggag
    13081 agaaccgaag ggaaagctta ccctgtttct cggacctttc cagcccgagc ttgaagaggc
    13141 tttttcccta tgggtcagag agagaaagga aaaggaccct ttggcccccc tcgtggttct
    13201 agtcgggtcc aatttgctgg gcctttacct gcgaaggctg ctggctcagc ggggcctgaa
    13261 tcatatcaac ctccgtttcc tcaccttcat cgacctggcc cgaactttgg ccgctgaacc
    13321 tctggatcgg aaagggctcc ggcctcttcc gcgatacggc gatcttgtcc tcatttctgc
    13381 cctggccgaa aa
//
LOCUS       DASPRR010000257         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1506458, whole genome shotgun sequence.
ACCESSION   DASPRR010000257 DASPRR010000000
VERSION     DASPRR010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506458"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VEL68_10070"
     CDS             complement(<1..1112)
                     /locus_tag="VEL68_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36353.1"
                     /translation="MRKAMPVAILLLAVLTGWGPAWGAEPIKLGAFFDLTGPSSAIGT
                     PTKLVAEMVVKKINGEGGINGRPLELVIADDEGDPTKAAIIAKKFIESDKVVAIVGPT
                     RTDTGMASKPIIEQMKVPTFMCVGGDPVITVPPFKWTFKSPQRTSVAVKKTYDYLKKK
                     GIQKIAIITSADGFGRDGKNWLDKLAPDFGVKIISSESFQATDSDMTAQLVKIKAANP
                     EAIICWTIGKAGSIVAKNVKQLAIKAPLYQCHGLPDPIYIKLAGDASEGNIMPSTKLM
                     VADQLPDSDPQKKVIQEFVRLYKDVYKYDTQFPINTHSGYAWDAIYIVANAMKKAGTD
                     NEKLRDAIEATKGYVGISGTYNLTAEDHNGLGTDSMV"
     gene            complement(1272..2558)
                     /locus_tag="VEL68_10075"
     CDS             complement(1272..2558)
                     /locus_tag="VEL68_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXZ36354.1"
                     /translation="MKTFMPSLKSEEELQKVQLEGLKWTIQHAYEGSPFYRKRLEETG
                     GRPGTIRSLEDIQKLPFTTADDLKEGYPFPLLSVPLEKVIRVHASSGTTGKRKVLCYS
                     ERDISDWADMFARCYEMAELAREDRVQVAVGYGLWTAGVGFQMGVERFGAMAVPVGPG
                     NLDMHCEVMVDLQTTVFCCTASMGLLMAEEIRKRGLQGKINLKKVIFGAERHSDAMRR
                     NIKEYLRAEDTFDIPGLTELYGPGTGLECKAHEGIHYWADYYILEVINPETLKPSLPG
                     EVGEMVVTTLRKEAAPLIRYRTRDLTRLIPGRCSCGNILPRHDKILGRSDDMFIIRGV
                     NIYPGSIDHLLSQEPRVGSEFQVLLERGEDGKDYMAVKVERAEKGNPKGDRAVADHLE
                     YLFRHQLLVSGKVEIVDFQSLPRTERKSKRVFDNRK"
     gene            complement(2645..>3322)
                     /locus_tag="VEL68_10080"
     CDS             complement(2645..>3322)
                     /locus_tag="VEL68_10080"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXZ36355.1"
                     /translation="MQKKGINPRTLSLKIGLFGAEPWPEEVHRFIEANWGIKAYDIYG
                     VMELVEPGVAGECMAQAGLHIFEDHFLPEIIEPASGKPLEPGKEGELVLTTLTTNAYP
                     LLRYRTSDVTALHLEPCSCGRTLARMDRVLSRSDQMITVRGINVFPEKIHEVLASFQG
                     IGPVYTTSVSKKKGMNDQLKIEMEASAEIMSAPEDRREALRDSLHLALRRAIGLRVEV
                     RLKAQGV"
BASE COUNT          640 a          976 c          885 g          821 t
ORIGIN      
        1 accatggagt cggttcccaa cccgttgtgg tcttcggcgg taaggttgta ggtcccgctg
       61 atccccacgt agcctttggt cgcctcgatg gcgtcgcgta gcttttcatt gtccgtcccg
      121 gccttcttca tggcgttggc cacgatgtag atggcgtccc aggcataacc ggaatgggtg
      181 ttgatcggga actgggtatc gtacttatag acatccttgt aaagacggac gaactcctga
      241 atgacctttt tctgggggtc tgagtcggga agctggtcgg ctaccatcaa tttcgtcgag
      301 ggcatgatat tcccctcgga agcatctccc gccagtttga tatagatggg atcgggaagt
      361 ccatgacact ggtaaagggg ggccttgatg gccagctgct tcacgttttt ggccacgatg
      421 gaccccgcct tgccgatggt ccagcagatg atggcctccg ggttagcggc cttgatcttt
      481 accagctggg cggtcatgtc gctgtccgtg gcctggaacg attcgctgga aatgattttc
      541 acaccgaaat cgggggccaa cttgtcgagc cagttctttc cgtcccggcc gaatccgtcc
      601 gccgaagtga tgatggcgat tttctggatt ccttttttct tcaggtagtc ataggtcttc
      661 ttcaccgcca ccgaggttct ctggggagac ttgaaggtcc acttgaaggg agggacggtg
      721 attaccgggt ctccgcccac gcacataaag gtggggacct tcatctgctc gatgatcggc
      781 ttggaggcca tgccggtatc cgtccgggtg ggcccgacga tggccacgac tttgtccgac
      841 tcaatgaact ttttggcgat gatcgccgcc ttggtgggat caccttcgtc atcggcgatg
      901 accaactcga gggggcggcc gttgatcccc ccttccccgt tgatcttctt caccaccatc
      961 tcggccacca gcttggtggg cgtgccgatg gccgaagaag gcccggttaa atcaaagaag
     1021 gctcccagct tgatcggctc cgctccccat gccggacccc agccggtcaa aacggctaac
     1081 aacagaattg cgactggcat tgcttttctc atgacaccct cctcctttgg attgcggatt
     1141 gcggatttcg gattggaaaa accacaatca ctctccttga tgaaaaacag ctttcagctt
     1201 tcagcagtca gcgatcagct aaaacaaaaa cagattcttt ggtcttaggc tgaccgctga
     1261 tagctgaaag cttatttccg attatcaaaa accctttttg actttctctc cgtccgtggc
     1321 aggctttgga aatcgacgat ctccactttc ccgctcacca ggagctggtg gcggaaaagg
     1381 tactccagat ggtccgccac ggctctgtct cctttgggat tccccttttc cgccctctcc
     1441 acttttaccg ccatgtagtc tttcccgtcc tccccccgct ccaatagaac ctggaattcg
     1501 cttcccaccc tgggctcctg ggaaaggagg tggtcaatcg agccgggata tatgttaacc
     1561 ccccggatga tgaacatgtc atccgagcgg ccaaggattt tgtcgtgccg ggggagaatg
     1621 ttgccgcagg agcaccgtcc tggaatcaac cgggttaggt cgcgagtccg gtaccggatc
     1681 aggggggccg cttctttcct cagcgttgta accaccattt cccccacttc tcccggtaaa
     1741 gacggcttca aggtctcggg gttgataacc tccagaatat aataatcggc ccaatagtgg
     1801 atgccttcat gggccttgca ctctaggccg gtccccggtc catagagctc ggtcagaccg
     1861 gggatgtcaa aggtatcttc cgccctcaga tactccttga tgttccgccg catcgcatcg
     1921 ctgtgccgtt cggccccaaa gataactttc ttcagattga ttttgccctg gaggccccgt
     1981 ttcctgattt cttcggccat gagcagaccc atggaggcgg tgcagcagaa gaccgtggtc
     2041 tggaggtcca ccatgacctc gcagtgcatg tccaggtttc ccggccccac ggggacggcc
     2101 atggctccaa accgctccac ccccatttgg aagccgacgc ccgccgtcca gaggccgtat
     2161 cccacggcaa cctggactcg gtcttcccgc gccaactcgg ccatttcgta acagcgggcg
     2221 aacatgtcag cccagtccga aatgtccctt tcgctgtagc acaggacttt tctctttccc
     2281 gtggttcccg aagaagcgtg cacccggatg accttttcca ggggcacgga gaggagagga
     2341 aaagggtatc cttccttcag gtcatcggca gtggtgaagg gaagtttctg aatgtcttcc
     2401 agggaacgaa tcgtccccgg ccggcccccg gtctcctcta gcctcttccg atagaatggc
     2461 gacccttcat aagcgtgctg gatcgtccat ttcaggccct cgagttggac cttctgcaat
     2521 tcttcttctg acttcaggct aggcatgaat gttttcatat ccgattcatc tcctaaatat
     2581 gcataccgca aagtgcatgg tgcatggcga aataacattc ttcctttcgt ctttacgcta
     2641 tgctctatac gccttgcgct tttaatcgta cctctactcg cagcccgatc gccctcctca
     2701 gagccaggtg aagagaatcc cgaagggctt ctcttcggtc ttcgggggca ctcataatct
     2761 ccgcagaagc ttccatttca atcttcaatt ggtcgttcat tcccttcttt ttggacaccg
     2821 atgtcgtgta gaccgggccg atcccctgga aagaagcaag gacctcatgg attttctcgg
     2881 ggaagacatt gatcccccgg acagtgatca tctggtccga ccttgataac acccggtcca
     2941 tccgagccag ggtgcggccg caggagcagg gttccagatg gagggcggtg acatcgctcg
     3001 tccggtagcg caggaggggg taggcgttgg tggtcagggt ggtgagcacc agttctcctt
     3061 cctttcccgg ctccaggggt ttgcccgagg ccggctcaat gatttcgggg agaaaatgat
     3121 cctcaaaaat gtgcaatccc gcctgggcca tgcactcccc tgccacccct ggttctacca
     3181 gctccatgac cccgtaaatg tcgtaggctt tgatccccca gttggcttct atgaatcggt
     3241 ggacttcctc gggccagggc tccgctccga aaaggccgat tttcagggag agggtccgcg
     3301 gattaatccc tttcttctgc at
//
LOCUS       DASPRR010000258         5304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_546814, whole genome shotgun sequence.
ACCESSION   DASPRR010000258 DASPRR010000000
VERSION     DASPRR010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546814"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VEL68_10085"
     CDS             complement(<1..1197)
                     /locus_tag="VEL68_10085"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ36356.1"
                     /translation="MSRKLHQKARHLLSQEHRILPLPDFKNRGGELSIALAYPNRYYT
                     AMSNLGLQAVYWLFNQAPGTLCQRVFLPDPEDIPEYRRTDTPLFSLESQRPVRSFDIL
                     AFSVSYENDYPHLLTMMDLARIPLLKKDRAEGGPLVMAGGASILMNPEPLADFVDFFV
                     IGEAEEIVGPLTETLRKGLEKGLSKESLLADLAHLEGLYVPQFYTVTYKPDGTIESFT
                     PKTGIPPRVKKAWLKDLNASVTVSPLVTPNTELSNMLLLELSRGCRRGCRFCAGCYAY
                     FPYRHRDAGLLEKAAVQGKGIAEKVGLVGAAISDYPGIIPLGREILQGHIPLSFSSLR
                     VDSLSPELADLAFQSGQKTITLAPETGSERMRRIVKKGFTEDAILRAAETLAERGFNN
                     FRLYFMV"
     gene            complement(1293..2486)
                     /gene="ftsZ"
                     /locus_tag="VEL68_10090"
     CDS             complement(1293..2486)
                     /gene="ftsZ"
                     /locus_tag="VEL68_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXZ36357.1"
                     /translation="MIELEESRKAGANLKVVGIGGGGCNAVNTMIKSGLTGVEFIGAN
                     TDAQALTVCSAPIKVQLGVNLTKGLGAGANPEIGRNAILEDRDRIREILSGADMVFIT
                     AGLGGGTGTGGAPILASIAKEAGALTVAVVTKPFLFEGKRRQHQAEEGLKELRKSVDT
                     LITIPNQRLLNIAGKDMSLLEAFRKADEVLLQAVQGISDLITVEGLINLDFADVRTVM
                     SEMGMALMGTGAASGEKRAIEAAQRAISSPLLEDITITGARGILISVTGGPDLGIYEV
                     NEACMLIQDEAHEEANIIFGAVIDEKMREEIRITVIATGFGRAESKEEIVPGPISHPP
                     EREKVVVNFSRKEENREIPTFLRSEKPKEVQERIAKAGRGRISSNLGAEEEYDIPTFL
                     RKQAD"
     gene            complement(2700..3935)
                     /gene="ftsA"
                     /locus_tag="VEL68_10095"
     CDS             complement(2700..3935)
                     /gene="ftsA"
                     /locus_tag="VEL68_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXZ36358.1"
                     /translation="MAKKGKKENIIVGLDIGTTKVCAIVGEVTDSGVDIIGIGTHPSK
                     GMRKGVIINIDATVESIRKAVEEAELMAGTEISSVYCGIAGSHIRGFNSHGVVAVKNR
                     EVTENDVQRVIDAARAVAIPNDREVLHVLPQEFIVDDQDGILEPLGMSGVRLEAKVHI
                     VTGAGTSTQNIIRCCNRTGLEVHNIILEQLAASEAVLLPDEKELGVTLIDIGGGTTDC
                     IVFSMGAVRHTSSLGLGGNQVTNDIAVGLRTPLVESEKIKTKYGCALTSMVKKEEVIE
                     VPSVGGRRARSLSRQILAEIIEPRMEEIFTLVHREIVKSGYENLIPSGVVLTGGTASL
                     EGMPELVEQVFNLPVRRGYPSGVGGLMDVVNNPMYAAAVGLVLQGRRHGPAGRFKTQE
                     RKFWGKIVPRVKRWFGEFT"
     gene            complement(3988..4734)
                     /locus_tag="VEL68_10100"
     CDS             complement(3988..4734)
                     /locus_tag="VEL68_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HXZ36359.1"
                     /translation="MTLSVATVASLAFLGYTVHRYFQASGQLNIGEVKIMGCMNVLES
                     ELLDLAKIDFKANLLNLDLQEVSRLLAQHPWVEKAKVRRDFSAKALIIEVQERIPRAW
                     ILLDELYLMDRNGEVFKKAEPKEKMNLPILTGVTPQEWRQKDPRVMELLRQALDLLEN
                     LEGRKVFSLQDVSEVHVSKTKDLTIYTLQGGVPIRFGSGDYADKLNRLEKVLPDLRNK
                     LKNVESLALNYPKKVVVKMKEGRPERPHKS"
     gene            complement(4951..>5304)
                     /locus_tag="VEL68_10105"
     CDS             complement(4951..>5304)
                     /locus_tag="VEL68_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXZ36360.1"
                     /translation="VPKAEFYTYEAKYTDGLAEHLFPAPLPPRKYKKALDLGYQAHRL
                     LGCEGATRVDLLYREPGEFFVLEVNSLPGMTPLSLMPEIARGAGIPFPELVEKILMGA
                     RLRIPVRGPRDSAGR"
BASE COUNT         1007 a         1632 c         1408 g         1257 t
ORIGIN      
        1 caccataaag tagaggcgaa agttgttgaa gccgcgctcg gccagggtct ccgccgcgcg
       61 caggatggcg tcttccgtaa accccttttt gacgattcgc cgcattcgct ccgagcccgt
      121 ttccggggcc agggtgatgg tcttctgccc gctctggaaa gccaggtcgg caagttcggg
      181 ggaaagggaa tcgacccgca gggaggaaaa ggacaggggg atatgccctt gcaggatctc
      241 ccgcccaaga ggaatgattc cgggataatc ggagatggcc gcccccacca gccccacctt
      301 ctcggctatt ccctttcctt ggaccgccgc tttttccagc agcccggcat cccgatgccg
      361 gtaagggaaa taggcgtagc agcccgcgca aaaccggcat cccctccggc agccccggct
      421 gagttccagg aggagcatgt ttgaaagctc ggtgttgggg gtcaccagag gggagactgt
      481 gaccgaggca ttcaggtcct ttagccaggc ctttttcacc cggggaggaa tgcccgtttt
      541 cggggtgaag gattcgatcg ttccatccgg cttgtaagtt acggtgtaga actgggggac
      601 atagagccct tccagatgag ccaaatcagc caacaaggat tctttcgata aacccttctc
      661 cagccctttc cgcaaggttt cggtcagggg gccgacgatt tcctccgcct ccccgattac
      721 gaagaaatcc acgaagtcgg ccaggggctc cggattcatc aggattgaag cccctccggc
      781 catgaccagg gggccgcctt cggcgcggtc ttttttcaga agggggatcc tggccaaatc
      841 catcatcgta aggagatggg ggtagtcatt ttcatacgag acggaaaaag ccaggatgtc
      901 gaaggatcgg accggtcgct gagactccag ggagaagagg ggagtatccg ttctgcggta
      961 ctccgggata tcctccgggt ccggaagaaa gactctctgg cagagcgttc cgggggcctg
     1021 gttgaagagc caatagaccg cctggagtcc caggttggac atggccgtgt agtaccggtt
     1081 gggatacgcc agagcgatgg aaagctcccc tccccgattc ttaaaatcgg gaaggggaag
     1141 gatcctatgt tcctgggaaa ggaggtggcg ggctttttgg tgtagctttc gggacataac
     1201 gacagctttc agctatcagc ttccagccat cagcagacag tttcttggtc tttcgctgaa
     1261 agctgaccgc tgaccgctga aagctgcttt tctcaatccg cctgcttcct taaaaaggtg
     1321 gggatgtcgt actcctcttc tgccccgagg ttggaggaga ttcggccccg gcccgccttg
     1381 gcgattcgct cttggacctc cttgggtttc tccgaccgca ggaacgtggg gatttcccgg
     1441 ttttcctcct tccgggaaaa gttcaccacc accttttcgc gctccggagg atgagaaatg
     1501 ggtcccggaa cgatctcttc cttgctctcc gcccttccga atccggtggc tatcaccgtg
     1561 atccgaatct cttctctcat tttctcatcg atcaccgcac cgaagataat gttggcctcc
     1621 tcatgagctt catcctggat gagcatgcag gcttcattga cctcatagat tcccaggtct
     1681 ggccctccgg tcacgctgat cagaattccc cgtgctccgg tgatggtaat gtcttccaag
     1741 agggggctgg agatggctcg ctgggcggct tcgatggccc tcttttcccc gctggccgcc
     1801 cccgttccca tcagggccat tcccatctcg ctcatgaccg tccgcacatc ggcaaaatcc
     1861 aggttgatca acccttccac ggtgatgaga tcggagatcc cctggaccgc ctggagtaga
     1921 acctcgtcgg ctttgcggaa ggcttccagc agggacatgt ctttcccggc aatgttcagg
     1981 agcctctggt tcgggatggt gatcagcgta tccacgcttt ttcgcagctc cttcaaaccc
     2041 tcctcggcct ggtgctgtct ccttttccct tcaaacagaa aaggtttggt gaccaccgcc
     2101 acggtcagcg cacctgcttc tttggcgatc gaggccagga tcggcgcccc cccggtcccc
     2161 gttcctcccc ccaggcctgc ggtgatgaag accatatccg ccccggacag aatctctcga
     2221 atccggtcgc gatcttccag gatggcattc ctcccgattt ccgggttcgc cccagcaccc
     2281 agccctttgg tcaggttgac gccaagctgg actttgatcg gggcgctgca gacagtcagg
     2341 gcctgggcgt cggtgttcgc tccgatgaac tccacgcccg tcagtccgga cttgatcatg
     2401 gtgttgactg cgttgcatcc ccctccgccg atccccacca ccttcaagtt ggccccggcc
     2461 tttcgactct cttccagttc aatcattccc ccacctcctt aaatcaattt cggattgcgg
     2521 attgcagatt tcggattgaa tcttttttgt aacctttccc ctcagggttt gggaagagtg
     2581 acagggaagc ttccgaaatt cgcaaccccc gccccgcatt tgctcagagt ctatagttta
     2641 gttccttccc cattttttct gggtccaatc cgcaattcga aatccgcaat ccacaatcgt
     2701 taggtgaatt ccccaaacca ccgtttcacc cgaggaacta ttttccccca aaacttgcgc
     2761 tcctgggtct tgaatcggcc tgcgggacca tggcgcctcc cctggagaac caggcccacc
     2821 gctgcggcgt acatgggatt gttaaccacg tccatcagcc ccccgactcc cgatgggtac
     2881 ccccgcctca caggaaggtt gaagacctgc tccactaact ccggcattcc ctccaaagag
     2941 gcggtccccc cggtcagaac gactcccgaa ggaatcaggt tttcataccc ggactttacg
     3001 atctcgcgat ggacaagggt gaagatttct tccatccggg gctcgatgat ttccgccagg
     3061 atctggcggg atagggaacg ggcccgcctg ccgccaacgc ttggcacctc gatgacttct
     3121 tcctttttga ccatggaggt cagggcgcat ccgtatttgg tcttgatctt ctccgactcc
     3181 accagagggg tccgaaggcc tacggcaatg tcgttggtca cctggttgcc ccccaacccc
     3241 aggctggagg tatgccggac cgcccccatg gaaaagacga tgcagtccgt ggtgcccccc
     3301 ccgatgtcga tcagggtgac ccccagctcc ttttcatccg gcagaaggac cgcttcgctc
     3361 gccgccagct gctccaggat gatgttatgg acctccagcc ccgttcggtt gcagcagcgg
     3421 atgatgttct gagtcgaggt cccggctccg gttacgatat ggaccttggc ctctaaccgc
     3481 actccggaca tacctagggg ttccaggata ccatcctggt catcgacgat gaattcctgg
     3541 gggaggacat gcaaaacctc ccggtcattg gggatggcca ccgcccgggc ggcatcgatg
     3601 acccgctgga catcgttttc ggtcacctcc cggttcttaa ccgccaccac tccatggctg
     3661 ttgaaccccc ggatgtggct cccggcaatt ccgcagtaga ccgagctgat ctccgtcccg
     3721 gccatcagct cggcctcttc caccgctttg cggatggatt ccacggtagc atcgatatta
     3781 atgatcaccc ctttgcgcat ccctttagaa ggatgggtgc cgattccgat gatatcgact
     3841 ccggagtcgg tcacctcccc cacgatggcg cagaccttgg tggtcccgat atccagcccg
     3901 acgatgatgt tctctttctt tccttttttg gccatggggg ctctcccctt ttttgagtcc
     3961 gggtccttta aattgggacc aaagaggtca ggatttatgg ggtctctctg gcctgccctc
     4021 tttcatcttg accaccactt tcttgggata gttgagggcc agggattcca cattcttcag
     4081 cttattccgc aggtccggaa ggaccttctc cagtcggttc aatttgtctg catagtcccc
     4141 gcttccaaag cgtatcggaa ccccgccctg caaggtatag atggttaaat ccttcgtttt
     4201 gctcacatgg acctccgaga catcctgaag ggaaaagact tttcgcccct ccaggttttc
     4261 caggaggtcc agggcttgtc ggaggagttc catgactcgc gggtctttct gcctccattc
     4321 ctggggagtc accccggtga ggatgggaag gttcatcttt tctttcggct cggccttttt
     4381 aaaaacttcc ccgttccgat ccatcaggta gagttcgtct aaaagaatcc aagcccgggg
     4441 aatccgctcc tgaacctcaa tgatcagcgc cttggcggaa aaatcccgtc tcacctttgc
     4501 tttttccacc cagggatgct gggctaggag gcggctcacc tcctgaaggt ccaggttgag
     4561 cagattcgcc ttaaaatcga ttttggccaa gtcgagaagt tcagattcca gaacgttcat
     4621 gcaacccatg atcttcactt ccccgatgtt cagttgcccg gaggcctgaa agtaccggtg
     4681 aactgtgtat cccaagaaag ccaggctggc cactgtagcc acgctgaggg tcacccttcc
     4741 cgcacggacg acccaacccc caagcccctt tttcccttgc ttttttggtc tcgtttttct
     4801 tcgctgtctt ccgatccgat acctgctggc catttctctt gattttattc agcatagctt
     4861 caaatccctc cactcctttt ttaaaaaaga gggaagggcg atactcatcc cggtggcgat
     4921 cctcaagctg gatgcgatcg gcctctcttt tcaccttcct gccgaatccc tcggcccgcg
     4981 gaccgggatt ctcaggcgcg ctcccataag gattttctcc accagttcgg ggaaagggat
     5041 ccccgccccg cgggcgatct ccggcatgag gctcaggggg gtcatcccgg ggaggctgtt
     5101 gacctccagt acaaagaatt ctccgggctc ccggtaaagg aggtccaccc gtgttgcccc
     5161 ttcacagccc aggagccggt gggcttgata ccccaagtcc aaagccttct tgtatttcct
     5221 cggaggaagg ggcgcaggga acagatgttc cgccagtccg tcggtgtact tggcttcata
     5281 agtgtaaaat tcggccttgg gaac
//
LOCUS       DASPRR010000259         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2153916, whole genome shotgun sequence.
ACCESSION   DASPRR010000259 DASPRR010000000
VERSION     DASPRR010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2153916"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VEL68_10110"
     CDS             complement(<1..338)
                     /locus_tag="VEL68_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ36361.1"
                     /translation="MLEVKNLEVIFPTLWGTVRAVHNVDLRQETGETVGLVGESGAGK
                     SMLGRSLMGLIPYPGQIPRGQIFWKGEDLRQKREKEMRKIRSKEIAMVFQDPISALNP
                     LKKIGDQLLEA"
     gene            complement(690..1904)
                     /locus_tag="VEL68_10115"
     CDS             complement(690..1904)
                     /locus_tag="VEL68_10115"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ36362.1"
                     /translation="MPGKALEGIRVLDLTRILAGPYCSMLFADLGAEVIKIEPPEGEM
                     IRDNPPVVENGKGGPHDRSRSAYFLSLNRNKYGITLNLKHPRAVSLFKDLVKISDVVL
                     ENYAPGVMKRLGIDYEVLKEINPRIIMCGISGFGQWGPYSDKIAFDIVAQATSGLMSV
                     TGYPDTPPTRVGTSLGDINAAVHAAFAIMAALWHRERTGEGQYVDVSMQEAMVSILEG
                     AIARWTVGGELMGPIGSHNTNESPFAAYKCKDGYIIIATVGNEHWERFCRAIGKPEWF
                     NDPKNATKGLRWARKWELSKEIEAVTVQYSVKEMGEMMDRERVANSPIFNIQQVVEDP
                     HLKERGYFVEIDHPIIGRAKLSGFPFKLSKTPGQIERTSPLVGEHNELILGKYMGFKK
                     DAVEQLKKEGAI"
     gene            complement(2493..>3465)
                     /locus_tag="VEL68_10120"
     CDS             complement(2493..>3465)
                     /locus_tag="VEL68_10120"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ36363.1"
                     /translation="LKHPRALRIFKDLVKISDIVLENYAPGVMKRLGIGYPVLKEINP
                     RIILGSISGFGQTGPYSERMAFDVTSQAMSGLMSITGHPGAPPTRVGTSLGDVNASVH
                     AAFAIMTALWHREKTGKGQWVDVSMMETMVAILEGGIVRWTIGKELLTPIGSMNPHEA
                     PMAAFKCKDGYIIIATVGDEHWQRFCRAVNRPDWAVDPSLQTKAQRWAKKYEIQEMVE
                     KITTQYTIKEVGEMMDKERVANSPILNIQQVVDDPHLNARGYFEEVEHPVIGKAKIPG
                     IPFKMSETPGTVERPSPLVGEHNELILGKYLGIGKGEIDRLKGEGAV"
BASE COUNT          730 a         1001 c          884 g          850 t
ORIGIN      
        1 gcttccagga gctgatcgcc gatctttttc aagggattga gggcggagat ggggtcttgg
       61 aagaccatgg cgatctcttt gctgcggatc ttccgcatct ccttctccct cttttgccgc
      121 agatcttccc ccttccagaa gatctgcccc cgggggatct gtccagggta gggaatcaat
      181 cccatgagag accgtcccag catgcttttc cctgccccgc tttcccccac gagcccgacg
      241 gtctcccccg tttcctggcg gagatccacg ttatggaccg ccctcaccgt gccccacaat
      301 gtgggaaaaa tgacttccag atttttgact tccagcaagc ccattatttt ggcaaggatc
      361 cggggttgag agcgctcttg ggaagggaat cattctcgaa tattcagcct agaggatcga
      421 atagatccat ccgggtaaag cgaaaaaaga atagccttaa aaatgcagga tcgtcaatga
      481 aaaagatttc cccagagggc tcaagaacaa gtaggaaagc cgggagggtt attgaaattc
      541 ttgactattt tattccttgc aggaggccgt cccatgtctt attcgccatt tcgttcaact
      601 ccggactcct tacatgcacg ccgccgcgtg cccctacaaa cttctaactt tttgttttat
      661 tccgtatctc gcatgaccca ttccgaattt cagatggctc cttctttctt aagctgttct
      721 acggcatctt ttttaaaccc catatatttt cccaggatca gttcgttgtg ctccccgacc
      781 agcggggaag tccgctcgat ctggcccggc gttttggaaa gcttgaaggg aaagcccgag
      841 agtttggccc gcccgatgat gggatgatcg atctcgacga agtatccccg ttccttcagg
      901 tgagggtcct cgaccacctg ctggatgttg aagatgggag agttggccac ccgctcccgg
      961 tccatcatct ctcccatttc tttgaccgag tactggacgg taaccgcctc gatttctttc
     1021 gacagctccc attttctcgc ccagcgcaat cctttggttg cgttctttgg atcgttgaac
     1081 cactccggtt ttccgatggc ccggcagaaa cgctcccaat gttcgttccc cacagtggcg
     1141 atgatgatgt agccatcctt gcacttataa gccgcgaaag gagactcatt tgtgttgtgg
     1201 gaaccgatgg ggcccatgag ttctccgccc actgtccagc gggctatagc cccttccaga
     1261 atggaaacca tggcctcctg catggacaca tccacgtact ggccctcccc ggttctctcc
     1321 cggtgccaca aagcggccat gatggcgaag gcggcgtgga ccgcggcatt tatgtctccc
     1381 agggaagtcc caaccctcgt agggggggtg tccggatagc cggtgacgga catgagcccg
     1441 ctcgttgcct gggccacgat gtcgaaagcg atcttatccg aataaggtcc ccattgcccg
     1501 aaaccgctga tgccgcacat gatgatccgg ggattgatct ccttcagcac ttcgtagtca
     1561 atccccagcc gtttcatcac cccgggggcg taattttcca ggaccacatc gctgatcttc
     1621 accaaatcct tgaagaggct caccgccctg ggatgtttga ggttaagggt aattccatat
     1681 ttattccggt taagggaaag aaaataggcg ctccggctcc ggtcgtgcgg acctcccttg
     1741 ccattctcaa ccaccggggg gttgtcccgg atcatctctc cttccggtgg ctcgatcttg
     1801 atgacctccg ctcccaggtc ggcaaagagc atggaacagt agggacccgc caaaattctg
     1861 gtaaggtcca aaacccgaat tccttccaga gcttttccag gcattccatc gattcctttc
     1921 gttcaaaaat tgatcctaca cgccggtcct tctgcccgac cgctcctttt tcccctcaac
     1981 ctttgaaccg cttttgaatt ccacctgaag gattcttcag gaaatgagac tgtggcaggt
     2041 ccaagcattc acagaggttc gatgaagaaa cgaccagccg gagaagggcc gcaaacatcc
     2101 ttgttaaatc gatcttacca tagcacgaag gattttgacg gtcaaagaag tttcaactcg
     2161 gaggacggat tctccacaaa aatctgtcaa taagttgtga aaaagctgcg caaaggcctg
     2221 tgaagtaacg caatatcatt tatctttatg ttcactgccc atcttttagt caaaaaaaac
     2281 taaagaaaat tcaggagttc cttccatggg ttgggatgat tcgccctggc attgggcttt
     2341 cttattgcga caccctgaga tgccgattca tcaaccatct ccgggggggt atgcctgaat
     2401 aggaaggccc agcgcgtgtt tggcttctct cttcgagtcg agggattcgg gaagaaagag
     2461 gttatcggaa aacgggattg ggcaatcccg attcaaaccg cgccctctcc cttgagccta
     2521 tcgatctccc ctttgccgat ccccaaatac tttcccagaa tcaactcgtt gtgttctccc
     2581 acgaggggag agggacgctc gacagtgccc ggggtctctg acattttgaa agggatgccg
     2641 gggattttgg ccttgccgat caccgggtgc tcaacctcct cgaagtatcc ccgggcattc
     2701 aggtggggat catcgacgac ctgctggatg ttgaggatgg gggagttggc cacccgttcc
     2761 ttgtccatca tctcccccac ttccttgatc gtgtactgcg tggtgatctt ctcgaccatt
     2821 tcctggattt catatttctt cgcccaccgc tgggccttgg tctgcaggct ggggtcgacc
     2881 gcccagtcag gccggttgac ggcccggcag aaacgctgcc agtgttcgtc tccaaccgta
     2941 gcgatgatga tgtaaccgtc tttgcatttg aaggcggcca tgggcgcttc atgggggttc
     3001 atgctcccga tgggagtgag gagttccttg ccgatggtcc agcggacgat ccctccctcc
     3061 aggatcgcca ccatggtctc catcatggag acatccaccc attgaccctt tcccgtcttc
     3121 tcccgatgcc agagcgcggt catgatggca aaagccgcat ggaccgaagc gttcacgtcg
     3181 cccagcgaag ttcccacgcg ggtgggagga gcgcccggat ggccggtgat agacatgagg
     3241 ccgctcatgg cctgcgaagt gacatcaaag gccatccgtt ccgaataagg ccccgtctgt
     3301 ccgaacccgc tgatgctgcc caggatgatc cgcgggttga tctccttcaa aactgggtag
     3361 ccgatgccca accgcttcat caccccgggg gcgtaatttt ccaggacgat atccgagatc
     3421 ttgaccaggt ccttgaagat cctcagagcc cgcggatgtt tcagg
//
LOCUS       DASPRR010000260         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2314859, whole genome shotgun sequence.
ACCESSION   DASPRR010000260 DASPRR010000000
VERSION     DASPRR010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314859"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VEL68_10125"
     CDS             <1..492
                     /locus_tag="VEL68_10125"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXZ36364.1"
                     /translation="PFHVLRYTDGVNLNPRNIRGGSLDVEDATTFTIFQEENATLAVD
                     QLPMPKKDFLARQTGSQQFGTIHGKVKDSSGKSVLKGSLVLWEGIQGKIIPFEDGIYS
                     LLGLNDGDYRVTIRAEGYGEVEKNLQARKGEVKEVDLVLPPRRTPQPPPSPKTNPPAR
                     TKP"
     gene            489..1496
                     /locus_tag="VEL68_10130"
     CDS             489..1496
                     /locus_tag="VEL68_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36365.1"
                     /translation="MRKGRAFSLLSGGLDSLLATRLIMDQGIEVVALHFITPFFGYQK
                     KGQEARYQERWERLYGIHARVIDVGDEYLRVLRNPRYGYGKNFNPCIDCKIFLFSRAK
                     AMMEEEKMDFLISGEVLGQRPMSQRRDTMRIVERDSETEGVLLRPLCAQLLKPTRPEL
                     EGLVGREKLLAITGRGRKPQMELAERMGVRHYPAPAGGCLLTDPELANRFRKCFRQSP
                     SITVEEILLLQVGRHFRLSEDRHLVVGRREEENQRLLDLSREGDTLLRVQGIPGPLGL
                     LRGPADEEDLRLAASIVARYSKAKNQEKVEVQYGKNGEDFRGLLRVPPAKDEQIPTLR
                     Y"
     gene            complement(1503..2486)
                     /locus_tag="VEL68_10135"
     CDS             complement(1503..2486)
                     /locus_tag="VEL68_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36366.1"
                     /translation="MKNFTDWERRERERKVKEIVRSFLLAFLAFRRLHGEYQKGSLCF
                     SDLAHFVDDRGQSILFALKESCHSLFRGSNAGVSEKEQVFDLIVGTLFHLAMKLREDL
                     YQLEFYGPKFTRLSEKNEGHPDPKGLVRQFQELISRARRALQEEMEEMDHLFKEILPR
                     FQDLLREYRENGLLLRFLLEEKELLREVWGGKALETILEMLYGPDSAQPDRLAGESYF
                     QSAFYPQATQAFTRALNKNPGDEEIQFLYHLSRGMDQFHCLASLSALESFEKCLSLCI
                     SDELLERYRPTILTACQRFGQECSGRRKNDQDPELVARAQSLQQRWDQNTK"
BASE COUNT          594 a          752 c          786 g          546 t
ORIGIN      
        1 cccttccacg ttctgcggta caccgacggg gtcaacctca atccccggaa catccgcgga
       61 ggaagcctgg atgtggaaga cgccaccacc ttcaccatct tccaggaaga aaacgccacc
      121 cttgcggtag accagcttcc tatgcccaag aaggattttc tggcgcgtca gacgggttcc
      181 cagcaattcg gcacgatcca tggcaaagtc aaggattctt caggaaaatc cgtcctcaag
      241 ggaagcctgg tcctttggga aggaattcag gggaaaatca tcccctttga ggatggcatt
      301 tactctcttc tggggctgaa tgacggggat taccgggtga ccattcgggc ggaaggctac
      361 ggggaagtgg aaaagaatct tcaggcccgg aaaggcgagg tgaaagaggt cgatctcgtt
      421 cttcctcccc gaaggactcc ccagccgcct ccctccccca aaaccaaccc gcccgcaagg
      481 accaagccat gagaaagggg agagctttct ccctgctctc cggagggctg gacagtcttt
      541 tggccacccg tctcatcatg gatcagggaa tagaggtggt ggccctccat ttcatcaccc
      601 ccttcttcgg gtatcagaag aagggccagg aggcccggta ccaggagcga tgggagcggt
      661 tatacgggat ccatgcccgg gtcatcgacg taggcgatga atacctccgg gtcctgcgga
      721 atccccggta cggatacgga aagaatttca atccctgcat cgactgcaag atctttctct
      781 tctccagggc caaagccatg atggaagagg agaagatgga tttcctgatt tccggggaag
      841 tcctggggca gcggcccatg tcccagcgcc gggataccat gaggattgtg gagagggatt
      901 cggaaacgga gggagttctc cttcgtcccc tctgcgccca gctccttaaa cctacccggc
      961 cggaactgga agggctggtg ggccgggaaa agctattggc catcaccgga cgaggacgga
     1021 agccgcagat ggagctggca gagcggatgg gggtccgtca ctatccggct cccgcgggcg
     1081 gatgtctgct caccgatccg gagctggcca accggtttcg gaaatgtttc cgccaatccc
     1141 cttcgatcac cgtggaggaa atccttcttc tccaggtggg gaggcatttc cggctttccg
     1201 aagaccggca tctggtcgtg ggcaggaggg aagaggagaa ccagaggttg ctggacctct
     1261 cccgggaggg ggacaccctc ctgcgggtgc aaggaatccc ggggccgctg ggtcttctcc
     1321 ggggaccggc ggatgaagag gacctaaggc tggcggcctc catcgtagcg cgttacagca
     1381 aggccaagaa tcaggaaaag gttgaggtgc agtacggaaa gaacggggaa gacttcagag
     1441 ggctcctccg ggtccctccc gccaaggatg agcagattcc caccctgaga tattgaatcc
     1501 cgtcacttcg tgttttgatc ccaccgctgc tggagagatt gggcccgggc gaccagctcc
     1561 gggtcctggt cattctttct ccttccggag cattcttgtc cgaacctctg gcaggcggtg
     1621 aggatggtgg gccggtatct ctccaggagc tcatccgata tgcaaaggga aaggcatttt
     1681 tcgaaagact ccagagccga cagggaagcg aggcaatgga actggtccat gccccggctc
     1741 aggtggtaca ggaattgaat ctcttcgtcc cccggattct tattcaaggc ccgggtgaaa
     1801 gcctgagtgg cctggggata aaaggcactc tgaaaatagg actcgccggc caggcggtcc
     1861 ggctgcgccg agtcagggcc gtacaacatt tccaggatcg tttccaaagc ctttcccccc
     1921 caaacctccc gcaacaattc tttttcttcc aacaggaaac gaaggagaag gccgttctct
     1981 cggtactcgc ggaggaggtc ttgaaagcga ggaaggattt ccttgaaaag gtggtccatt
     2041 tcctccatct cttcttgcaa agccctccgg gcgcgggaaa tgagctcctg gaattgtcgg
     2101 accaggccct tggggtccgg atgcccctca tttttttcac tcaaccgggt gaacttgggc
     2161 ccatagaatt cgagctggta caggtcctcc ctcaatttca tggccagatg aaagagggtt
     2221 ccgacaatga ggtcgaagac ctgttccttc tcggaaaccc ccgcgttgct cccgcgaaaa
     2281 agggaatgac agctttcttt gagggcaaat agaatgctct ggccccgatc atccacaaaa
     2341 tgggccaggt cggaaaagca aagggagcct ttttgatatt cgccatgcaa ccggcgaaag
     2401 gccagaaagg cgaggagaaa ggagcggaca atctccttca ctttcctttc ccgctcgcgc
     2461 cgttcccagt ccgtgaagtt cttcatcgtc ttctcctgca ctatttaaca ttttatccat
     2521 gcttcccaga ggaaaacaag gggcttctca ggcctccgat ccctgtcggt agatggaatg
     2581 gaggtcaagt cctgggaggt cttgccgggc cccggggcga gggcattgta cgcagaaacc
     2641 ataaggcgcc tttgattcca tggcacagac gggcctct
//
LOCUS       DASPRR010000261         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1025962, whole genome shotgun sequence.
ACCESSION   DASPRR010000261 DASPRR010000000
VERSION     DASPRR010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025962"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VEL68_10140"
     CDS             complement(<1..243)
                     /locus_tag="VEL68_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003028244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HXZ36367.1"
                     /translation="MEKMEVKSLNSPEEVRTFDKGKLELVKVNGAMIGRAVFQPGWKW
                     SDSVKPLAKTKSCEAPHFQYHVSGTLRLHMDDGTERD"
     gene            452..1624
                     /locus_tag="VEL68_10145"
     CDS             452..1624
                     /locus_tag="VEL68_10145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXZ36368.1"
                     /translation="MENPNFDVIIVGGAVIGSSVAYHLLAAEPRLKVAVLERDPTYSR
                     ASTSLSLGGVRVQFSLKENILISLYAQEFFSRFEEEMAVEREKPSIDYRRAGYFFLID
                     RPGWSAAEKDMELQRRLGCEVEWWTPERIRRELPLFQADLFVGGTFGPKDGYLDPYGV
                     LVGFRKKAISLGARYIPYEAVEIKKIQGSTIRVKLASQEVLYSDTVVNAGGPWGAEVA
                     RMAGIELPLSPVNRQVFAVKPEVSIDRALPLVIAPSGLYFRSETGGLILVGRSMEDDE
                     VGFGFRWDWKRFTDRLWPELAQIVPAFEKLKLVRGWAGLYEVNLLDRNAILGPWPEME
                     GFYLINGFSGHGLQQSPAAGRYLAELILRRAPVLDLSCFSPRRILENRPLGEGAVV"
     gene            complement(1631..2611)
                     /gene="cysK"
                     /locus_tag="VEL68_10150"
     CDS             complement(1631..2611)
                     /gene="cysK"
                     /locus_tag="VEL68_10150"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXZ36369.1"
                     /translation="MAHFFSDNSLSIGNTPLVKINRISQHLKATVLAKIEGRNPAYSV
                     KCRIGASMIWEAEKRGLLQPGMEIVEPTSGNTGIALAFVCAARGYHLTVTMPETVSVE
                     RRKVLAAFGAHIVLTPAARGMTGAVQEAERILQSTPKAFMPQQFKNPANPDIHFRTTG
                     PEVWEQTEGRIDVLVSGVGTGGTISGVSRFIKKEKKHPLLSVAVEPRESPVISQRLAG
                     QELKPGPHKIQGIGAGFIPDTLDLSLVDRVEQVSSDEAIDLARRLAREEGILSGISCG
                     AAMAAALRLAAEKEFAGRTVVVILPDSGERYLTTSLFEGIFDEAGLPRGK"
     gene            2961..3176
                     /locus_tag="VEL68_10155"
     CDS             2961..3176
                     /locus_tag="VEL68_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36370.1"
                     /translation="MKLKPLSAESDELRKIIISSNLTSCYIMLEELLASKGERLFFDK
                     FRARWIDIFKNVYDHVDRMKPRKDPRE"
     gene            3587..>3870
                     /locus_tag="VEL68_10160"
     CDS             3587..>3870
                     /locus_tag="VEL68_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXZ36371.1"
                     /translation="MKKRIRRIGFTLIELLIALFALSVVLLTLGPMVYSVMNSASRSK
                     ETAIEVGFASKSRSHKEIFYMAEAGLQDARSRLQTGASGFPIQDTMPSNP"
BASE COUNT          825 a         1048 c         1029 g          968 t
ORIGIN      
        1 gtctctttcc gtcccgtcat ccatgtgcag tcttagggta cccgaaacgt ggtactggaa
       61 atggggggcc tcgcagctct tggtcttggc taagggtttc accgaatcgg accatttcca
      121 accgggttga aagaccgccc gtccgatcat agccccattc actttgacca attccagttt
      181 ccctttgtca aaggtcctga cttcctctgg ggaattcagg cttttcactt ccattttctc
      241 cattttcgtt ccctcccctc accgaattaa actcctctgt atggttagat gcttatcctc
      301 tccttatgat ctccagcctt ccccgaattc cgattttctc catgatgaga aatggtatac
      361 tttgaggagg gccctttagg gatgacccat tcctgggtta tgcggaaatt ccgggatcag
      421 aaaagtattt cgggaagggt ccgagaaggc catggaaaac ccaaattttg acgtcatcat
      481 cgtgggcggt gcggtcatag ggtcatcggt ggcctaccat cttctggccg ccgagccgag
      541 gctcaaggtg gcggtattag aacgagaccc cacttactcc cgggcctcta cctctttgtc
      601 actgggcggg gtaagggttc aattcagcct gaaagagaat atcctgattt ctctttatgc
      661 ccaggaattt ttttcccggt ttgaagaaga gatggctgtg gaaagagaga aaccctcgat
      721 cgactatcgg agggccggat acttttttct catcgaccgg ccgggatggt ctgctgcgga
      781 aaaagacatg gaattgcaga ggaggctggg ttgtgaagtg gaatggtgga ccccggaaag
      841 gatccgaaga gagctccctc ttttccaggc cgatcttttc gtgggaggaa ctttcggccc
      901 gaaagatgga tacctggacc cttacggagt cctcgtggga tttcgcaaga aagccatctc
      961 ccttggggcc cgttacatcc cctatgaagc ggtagagatc aagaagattc agggaagtac
     1021 catccgggta aagctggcgt cccaagaagt gctctattca gataccgtgg tcaacgcggg
     1081 cggcccctgg ggagccgaag tggctcgaat ggccgggatc gagcttcccc tctctccggt
     1141 caatcggcag gtctttgcgg taaagcccga ggtctccatc gaccgcgctc tcccgctggt
     1201 catcgctcct tccggccttt actttcgttc ggagacaggt ggactcattc tcgtaggccg
     1261 atctatggag gatgacgaag tgggatttgg attccgctgg gactggaagc gattcaccga
     1321 tcggctttgg cccgaattgg cccagatcgt tcccgccttc gaaaaactga agctggttag
     1381 gggttgggcc ggcctctacg aggtaaacct cctggaccgc aatgcgatcc tcggtccttg
     1441 gccggaaatg gaagggttct acttgatcaa cggcttctcc gggcatggcc tgcagcagtc
     1501 tccagccgca ggccggtacc ttgccgagtt gatcctaaga cgggctccgg tcctggacct
     1561 atcctgcttc agcccccgca ggattctcga gaaccggcct ctcggagagg gcgcggtggt
     1621 atagggggct ttattttccc ctgggcaggc ccgcttcgtc aaaaatcccc tcaaacagag
     1681 aggtggtgag gtagcgctct ccggaatccg ggagaatgac cacaaccgtt cgaccggcga
     1741 attctttttc ggcggctagt cggagagccg cggccatggc cgctccgcaa ctaatccccg
     1801 aaagaatccc ttcctcccgg gccaatctgc gcgcaaggtc aatggcctcg tcgctgctca
     1861 cctgctcgac ccggtccacc agggaaaggt caagggtatc gggaatgaat ccggcgccga
     1921 tgccctggat cttgtggggc cccggcttga gctcctggcc tgccagacgc tggctgatga
     1981 ccgggctttc ccgcggttcc acggccacac tcaggagggg atgctttttt tctttcttga
     2041 taaaccggga gactcccgaa atggttcctc ccgtgcctac accggaaaca agcacatcga
     2101 tccggccttc agtctgttcc cagacctccg ggcctgtggt tcgaaagtga atgtccgggt
     2161 ttgccgggtt cttgaactgc tggggcatga aagccttggg ggttgattgc aggatgcgct
     2221 ccgcctcctg gactgctccg gtcatgcctc tagccgcggg agtgagaacg atgtgggctc
     2281 cgaaagcagc cagcaccttc ctacgctcta cagaaacggt ctctggcatg gtaacggtga
     2341 gatggtatcc gcgagcagcg caaacaaagg ccagagcgat tccggtgttg ccgctcgttg
     2401 gctcgacgat ttccatgccc ggttgcagaa ggcctctctt ttcagcttcc cagatcatgc
     2461 ttgccccaat ccgacacttg accgaatagg ccgggttccg tccctcgatt ttggccagca
     2521 ccgtagcctt taaatgctgg ctaattcggt tgatcttgac cagaggggtg tttcctatgg
     2581 agagggagtt gtcgctgaaa aaatgggcca tggggttctc ctttccatga gaaaagatga
     2641 ttttcgggaa tacctattct tatgataacc caccctttgc cctttcaacc aggaaattta
     2701 tgccggttgg caatcctttt tttcccttat aatgggtagg aaaagaaccc aaggcctgtt
     2761 tttggagcgg taggggaaag ctgagcaggc aaatcggggg gtttcaagaa tctttccaag
     2821 aaaaggattt cttcccttga tctaaatgga gttttagtgt ttaataaaaa acaaccttga
     2881 atggatggga ttcttcagat ttatttcatt ttctattgct tgttacaccc catgggaggc
     2941 ttacaaagga gaggtaaaaa atgaagctga aacccttatc ggcagagagc gatgaactgc
     3001 ggaagatcat tatttcttca aatctcacct cgtgttacat catgctggag gaacttctgg
     3061 cctctaaggg agaacggctc ttttttgaca agttccgtgc ccgttggatc gatatcttta
     3121 aaaatgttta tgaccatgtg gatcggatga aaccgcgtaa agacccgcgg gaatgatgct
     3181 caggaggatt tcctggcccc ctggagcggc ccctaccaag gttcttctcc tcgggacagg
     3241 attccactcc ggattaaagg caccccaact gcaaaaggag agatgtcctg gcttcacggg
     3301 gtctgagccc tccgagagag taaccgacct tccatagcag caatcttcct taccccctgt
     3361 tttcttgcaa ctattcggtt ttccatccat ttttgtcttc gtcctatcaa tttcaggcct
     3421 ctttttgttg ggcaccaagc catatttttt ttgcccgctc ctttggccct cctcttgaat
     3481 accgctcctg gcaagagaaa tcgttccctg gcatttattt tgctcctgcg cttacctgaa
     3541 ttcaagaagg cttgcccgga atgcctgccg gaccactgaa aacccaatga agaagagaat
     3601 aagaagaatc ggattcaccc tgatcgaatt gctcatagcc ctctttgccc tgtctgtggt
     3661 gctgctcacc ttaggcccca tggtctactc ggtgatgaat tctgcttcgc ggagcaaaga
     3721 gacggccata gaggtgggat tcgcctccaa aagcaggtcg cacaaggaga ttttctacat
     3781 ggctgaagcc ggtcttcagg acgcccggtc acggcttcaa accggggcca gcgggtttcc
     3841 cattcaagat accatgccct ccaacccaag
//
LOCUS       DASPRR010000262        13072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_375509, whole genome shotgun sequence.
ACCESSION   DASPRR010000262 DASPRR010000000
VERSION     DASPRR010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13072)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13072
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375509"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(413..682)
                     /locus_tag="VEL68_10165"
     CDS             complement(413..682)
                     /locus_tag="VEL68_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXZ36372.1"
                     /translation="MNKADLVAEVGKVVGSKRMADKAVSCVLEAIKKGLKKGQAVTLV
                     GFGTFSVGKRKARVGRNPQTGKEIKIPAKKVPKFAPGKALKTAVK"
     gene            1439..2902
                     /locus_tag="VEL68_10170"
     CDS             1439..2902
                     /locus_tag="VEL68_10170"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXZ36373.1"
                     /translation="MSAEKQTVDGGKGPRFEVEDFPEPLRDLTPRKLFAILGPAVIAL
                     GGTIGGGEWLIGPSLFVKWGLALLWITTVSSILQVFLNLEMTRYTLYTGEPVTVGFMR
                     LAPGKAFWGWLFTIVGFCERGLPGWALGTATAVAALQLGAIPGVADKGTVLFWGYVVF
                     ASCCVVITLGKSAERTLEWANWVMMIVVLGGLLLLDLYIVPASVWLEGVVGFFSFGYM
                     PKGVDVLLLGALVGYSAYGGFGNNAITNWYRDKGYGMGGKIGYIPALIGGKTIHVSPH
                     GKVAMPTAENISRWKGWWKLLNIDQWFIFYGGAMAGMFLPGILYVAVIPRGTTLPAWG
                     IAASSASGLVQQLGSFGWFLALFFGFWILYSTAISNVDLVVRQSTDMLWFASDRVRKL
                     AKMDIKKIYYILLLAFFIWGVSYVNLSVPLIILAVSANIANFTMALSAVLTIRLNRKF
                     LPKEFRPSLWRELVLVGNLIFFGFFFSVFVWVKILGF"
     gene            3068..4324
                     /locus_tag="VEL68_10175"
     CDS             3068..4324
                     /locus_tag="VEL68_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXZ36374.1"
                     /translation="MARLYEYQGKQLLKKAKVPIPQGEVAATPQEAGKIAEKIGKPVA
                     VKVQIWAGGRGKAGGIKIAEHPLDAQIAAQNLLGSEIKKMKVEKVLVEEKLNIDKEYY
                     VGVIIDSSMGVRSPVVMFSSEGGMEIETVPSEKIAQITVDVLRGFRLYDALNLAVQMK
                     VPTQFLPAIAQAIMGLYQTFKDYNCRTAEINPLVLTKDGKIVAADCRIAIDDSSVYRH
                     PELGIDVARESPTPPTELDKIAWWIEEGDLRGTCYIAQMVSEIKGLNYVGYHGLGGGG
                     AILGVDALNREGLKIADYADTSGNPTAAKVYRAAKVIISQPNIEAYFLGGFIVANQEQ
                     WHHAHGVVKALREELPKRPGFPCVLMLCGNREKEALEILREGTRDLPGRIEIYGAEKV
                     YETEFLAKRVKALIMEYRRDRGIKEG"
     gene            4329..4613
                     /locus_tag="VEL68_10180"
     CDS             4329..4613
                     /locus_tag="VEL68_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005026326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36375.1"
                     /translation="MHFEERTIKIIIDEEKCVGCKTHVCAQACKTYDRGILVIKDGKP
                     ALGGDSEFGKRVGTECLACEYECWFRGNKAITIEAPIPGLDEYKKKYGVP"
     gene            4644..5543
                     /locus_tag="VEL68_10185"
     CDS             4644..5543
                     /locus_tag="VEL68_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ36376.1"
                     /translation="MAILLKKDTTILVQGITGREGSARAAFMKGYGTKVLAGVTPGRG
                     GEEVSGIPVFNTVAEAIRKVGPIDASVTFVPGTGLKEAVLEAIDSGIKFIVSPAERVP
                     LYDILEMVAYAKQKGVRLLGPGCIGVLSPGIAAAGWLGGNPEYARRVFLPGPVGVMSR
                     SGGQSGTVPWAVKEVGLGVSTVVHIGTEPVLGTSFGDILPLFEKDPDTKGVAVFGEIG
                     GPYEEEAAECVRKREYTKPLVIFVAGAWAPEGMRFSHASSIIERGKGSAKSKIQSLKD
                     AGVHVVDRPDELAPTIKRLIQSQ"
     gene            5555..6451
                     /locus_tag="VEL68_10190"
     CDS             5555..6451
                     /locus_tag="VEL68_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXZ36377.1"
                     /translation="MPVMRSVFYIPGNNDKMIAKAPTTPADIITLDLEDSVPPAEKPK
                     ARDMVRENLKLAGTGGSTVYVRINNWETLMTNDDLEAIVHEGLSGVCLAKTGGPDHVQ
                     RLEWKLEELERRRGLPVGKIAIQLLIETAKGVINAYPSAIASKRVNSLIFGAVDYTKD
                     MRVKLTSEGVEQFYARSHTAVAARASGCIAIDCPFVDFKNIEAFEKSTREGRQMGYEG
                     RMLIHPTQIEPSNRIYSPAPEDVEWAKGVVKVFEEEGIAKGMAAVSYKGKMVDTPVYD
                     NARTILATLAEIEAFDKKRKQA"
     gene            6759..7679
                     /locus_tag="VEL68_10195"
     CDS             6759..7679
                     /locus_tag="VEL68_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ36378.1"
                     /translation="MENVSYLTPEIQALLGQEVTWKATEPVDAGKIHRFAKSLGLANP
                     IYYDIEKGQPVAPPTFLFSVNHDSLGDFDETGRPTKRLSLPPPFGPAMRGGNKYQFFQ
                     QVRVGDRLWMKRKVNGLEEKQGRTGALLFVPYDVQYTNKGGDLLGINTETLIFRVTQE
                     GKEKNRMSSHTVFEDRREGQEIPPYRVTVSKVQMMMYAAATWNPNQLHWDSDFCKKRG
                     FAEANIAGPMFGDYLAEMLVKWVGDPSRLKSLDYVNRNMAFPGDTLICRGKALGRPRQ
                     GNTELIDCQVWVENQKGEILVQGSGQVSLG"
     gene            8039..9217
                     /locus_tag="VEL68_10200"
     CDS             8039..9217
                     /locus_tag="VEL68_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXZ36379.1"
                     /translation="MLDEFHPPARLLMGPGPSNVDPRVNLAMAKPLLGHLDPYFIQIM
                     HQVQALLREVFATRNSLTIPISGTGTAGMEAAFVNVVEPGDKILIGVNGFFGERMVEI
                     ASRCGVEVKKLDGTWGKPFEPAEVEKELRAFRPKVLALVHAETSTGVLQPLEEFSSIL
                     KKFPETLFLADTVTSLGGHPVKVDEWGIDVCYSGTQKCLSCPPGLAPITFSPKAVERI
                     KNRTRKVQSFYLDMDTLGKYWGSEITYHHTAPVSMNYALLEALRIVQEEGLDRRFERH
                     RRNHLGLVAGIEAMGLKMFVDPPHRLWSLNTIAIPEGIDDGKVRKSLLEEYGLEIGGG
                     LGPLKGKIWRVGLMGYNSDEKNVLFFLTTLEQALRAQGFEVPPGVGTSAARDFYAKNK
                     "
     gene            9331..9900
                     /locus_tag="VEL68_10205"
     CDS             9331..9900
                     /locus_tag="VEL68_10205"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXZ36380.1"
                     /translation="MGQPWTFLYSKILYTCRIFSLKKEGYRSPRTGREHDFFLLESTD
                     WVNVIPRTADGRIILVKQYRIGTKDFSLEIPGGMLDEGDDPAGAAARELLEETGYAGE
                     EPILLGVVHPNPAIQTNRCYTFLINNVVFKTPPQQDSTEDIEVQFASLAHIPELIRKG
                     KITHALVIAAFYWYFSSTASKDSGAQGFA"
     gene            10197..11459
                     /locus_tag="VEL68_10210"
     CDS             10197..11459
                     /locus_tag="VEL68_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXZ36381.1"
                     /translation="MDVPLMSLIALFIAIVISCTTSLNIGTLAIGLSLIVGYYFGGVK
                     LAEIVKGYPTSLFIMLAGVTYLFAIAQTNGTLEKISKYAIKAVRGNVALLPIVLFFLA
                     FALSSMGPGQITISALLAAPAMLLAQEVGISPLLMALVVGNGAQAGAMSPIAPPGIIS
                     ASILSKMGITGVSMVLWMNMLITHVAVSVLAYFLFGGLKLLRIKDTGQRETLRNLPVE
                     PFTMEQVCTIIGVVILILGALFFKLDIGMGAFMIGAVLSLIKAVDEGKAIKAMPWGTI
                     LMVTGVTVLVQLMSQVGGMDLFATIMAKISTAYTVTMVAGFVAGVVSAYASTTGVILP
                     AFLPLAPGLLAKVGGTDLMALISSIVVCGFVVDLSPLSTTGAVFIANAAPQADKNKLF
                     RNMLIWGLSMSVVGAVISWLAFTVLGLP"
     gene            complement(11494..12651)
                     /locus_tag="VEL68_10215"
     CDS             complement(11494..12651)
                     /locus_tag="VEL68_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36382.1"
                     /translation="MQYGLTEEQVLLRDTVRKLAKEKVEPGAGARDQKGEFSWEMVEL
                     LRENGLFGVDFPEEHGGAGAGLLALVIVCEELSKAEASTGMLPVAQELGTLPILLAAN
                     PDQKKKFLPALASGEKLSAFALTEASGGSDVAAFRSKAVKKGDYYILNGTKTFITNGG
                     VADTVAVYALTDPAKGGHRGSSVFVVEKGTKGFSVGKHEDKLGIRASDTVELIFEDCP
                     VPAGNLLGGEGMGFAIMMKTLDFSRPAVGAQALGIAAGAFEYSLNYAKERIAFGKPVV
                     AQQGISFKLADMAMNIEAARQLLYKTASLLQELPKDMSRISPETIRLSSMSKCFCADT
                     AMRVTVEAVQVLGGYGYVKEYPVERYMRDAKITQIYEGTNEIQRVVIMSTY"
BASE COUNT         2842 a         3578 c         3668 g         2984 t
ORIGIN      
        1 gtctccacgg ggtttacccc gacggcgcaa atctttacta ccacctgctg gggaccgggc
       61 tgggggtctg gaacctcttc cagtttcatc acttcggggg gaccgaattc gtggatgcga
      121 atcgctttca tggccttctc ccttcgaatg aaaggattcc aatggagttg aggaatatac
      181 gccgcctgac tttgctcctc taccggatgt cctattgtat tctaacacgg gaaggaagct
      241 ctttcgtgct cccaaaacgg caggattcac taggaatgac ggggtgttgg aagggagagg
      301 cgggaaatag attttgtgaa aatccttctt tcgactaaaa atgaaaaagc gggacagaaa
      361 gtctccctcg atgattcgag atgctccctg tcccgcccgg aataactaag aattacttca
      421 ctgccgtttt cagggctttg ccgggtgcga atttgggaac tttcttcgcc gggatcttga
      481 tttccttgcc cgtctgaggg ttccggccta cccgtgcttt tctcttgccc acgctgaacg
      541 ttccaaagcc caccaaggta accgcctggc ccttcttcaa ccccttctta attgcttcca
      601 acacgcaaga gaccgcttta tcggccattc tcttgctccc caccactttg cctacctccg
      661 ccaccaagtc cgccttgttc aacttgcctc acccccttta ttctgatatt gagccaattt
      721 tccccctatt ctaatgcaat ttaaaaactt gtcaaatcaa aattatcaat ctcttacgat
      781 ttttttcaac ctcttcagaa ggctcccgtt ttcgagcaga tcgtttcttt ctcttgattt
      841 tcaacttgac aggccgcgac aaatatttta tataggggcg acagcctgac ctctttaccc
      901 atccacaaaa tattacccat tttcaagcgg ttggagaata ggtcttgttc aaaccgaatt
      961 tccaccatga agcgcatttt gattggaaat tttgccaacc accagatcga tccgccaccc
     1021 gggaaaaaaa tcatagacga ggtctatgtc tgcttctctc tctcactaat aatggaaaac
     1081 agaagccagt tcaacaatgt tctgcgcgcc aattattaac agaagggtta gggtaaagtc
     1141 gacagccaat ccccaggctc tgaagaagaa tgaaaagaaa gggttgatcg gggcaaccaa
     1201 tttcccgatt tccccattct tattttccat gaaggtttgc gcccctcatt ggacttgaac
     1261 cagagaagac tcctttgtaa ctggattgcc tttcctaacg atggtaagaa tttaaccaag
     1321 aaaggaggtg tttcatcttt tccctgtgta gattgcccag ggctgattca ggaaggattc
     1381 tacgggtaat ggggctatat tcagctttca accattgttc tgaaggagga aggtaaccat
     1441 gagtgcagaa aaacaaactg ttgatggagg caagggccca aggtttgagg tggaggattt
     1501 tccggaacct ttgcgggatc ttaccccgag aaagctattc gcgatcctgg ggccagccgt
     1561 gattgctctc ggcgggacta tcggcggggg ggaatggctg atcggacctt ctttattcgt
     1621 gaaatggggc cttgctctcc tctggattac caccgtatcg tccattcttc aagtgttcct
     1681 caatctcgag atgactcgtt acacccttta taccggagag cccgtcaccg tggggtttat
     1741 gagactcgcc cccggcaaag cattctgggg ctggctcttc acgatcgttg gattttgtga
     1801 gcgcggtctg cccggatggg ctctgggtac agcgaccgcc gtcgccgctc ttcagctggg
     1861 cgccatccct ggggtggcgg ataaaggaac agtcctcttc tggggttacg tggtttttgc
     1921 ttcctgttgc gtggtcatta ccctggggaa gagcgccgaa cggaccctcg agtgggccaa
     1981 ctgggtcatg atgattgtcg ttctgggtgg gctactcctg ctggacctct atatcgttcc
     2041 cgcctcggtg tggctcgaag gcgtggtggg atttttcagt ttcggctaca tgcccaaggg
     2101 ggtggatgtt ctccttctgg gtgccctggt cgggtattcc gcgtacggag gattcggcaa
     2161 caatgccatc accaactggt accgggataa gggctacggc atggggggaa agatcggcta
     2221 tatcccggcc ttgatcggcg gaaagaccat ccatgtttct cctcacggaa aagtcgccat
     2281 gccgacggcg gaaaatattt cccggtggaa aggctggtgg aagcttctga acatcgatca
     2341 gtggtttatc ttttatggcg gggccatggc cgggatgttc ctcccgggta tcctgtatgt
     2401 ggccgtaata cccagaggga cgacccttcc ggcctgggga attgccgcct cttcggccag
     2461 tggattggtc cagcagctcg gctccttcgg atggttcctg gccctcttct ttggtttttg
     2521 gattttgtat tcgaccgcca tcagcaacgt agacctggtc gttcgccagt ccacagacat
     2581 gctgtggttt gcatcggacc gcgtccgaaa actggccaag atggatatta agaaaatcta
     2641 ctatatcctc ctgctggcct ttttcatttg gggcgtgtcc tacgtgaatt tgtcggttcc
     2701 tctcatcatc ctagccgtca gcgccaatat cgccaatttc acgatggcgc tctcggcggt
     2761 cctgaccatc agactgaacc ggaagtttct gccgaaggag tttcggccca gcctctggag
     2821 ggagctggtg cttgtgggca atctcatctt cttcgggttc ttcttctcag tctttgtctg
     2881 ggtgaagatt ttagggtttt agggttagca cccgagaaaa gggctggcct gaccaagtgg
     2941 gggaaggggg gaggagtttt caattgaccc ctttcccctg ctcttgatag atttaagggc
     3001 agtgctgtta agggcatagg gggtgattcc ttgcccgaga aggaatcgta gcctttagga
     3061 ggtgggtatg gctcgattgt atgaatatca ggggaaacaa ttactcaaga aggcgaaggt
     3121 gccgattccc cagggcgagg tcgccgcgac cccccaggaa gccgggaaga tagcggaaaa
     3181 gatcgggaag ccggtagcgg tgaaagtgca gatctgggcc ggggggcgcg gaaaagcggg
     3241 gggcattaaa atcgccgaac atcccctgga cgctcaaatc gccgcccaga accttctggg
     3301 gtccgaaata aagaagatga aggtggaaaa ggttctggtg gaggagaagc tgaatatcga
     3361 caaggaatac tacgtcggcg tgatcatcga ctcttccatg ggggtccggt ccccggtcgt
     3421 catgttcagc agcgaagggg gcatggagat cgagaccgtg ccatcggaaa agatcgccca
     3481 aattacggtg gacgttcttc ggggcttccg cctgtacgat gcgttgaacc tcgccgtcca
     3541 gatgaaggtg ccgacgcaat tcctcccggc catcgctcag gccatcatgg gtctgtacca
     3601 aactttcaag gactacaact gccggaccgc tgagatcaat cctctcgtct tgaccaagga
     3661 cggaaaaatc gtggccgcgg attgccgtat cgccatcgac gactcctccg tttatcgcca
     3721 tccggaactg ggcatcgatg tggcccgcga gtctcccacc ccccccacgg aattggacaa
     3781 gatcgcatgg tggatcgagg agggcgacct ccgggggacc tgctatattg cccagatggt
     3841 gtcggagatc aaaggattga actacgtggg ctaccacggg ctcggcgggg ggggcgccat
     3901 tctgggagtg gatgccctca accgcgaggg gttgaagatc gccgactacg ccgacaccag
     3961 cggcaacccc acggcggcca aagtctaccg ggccgctaaa gtgattatct cccagcccaa
     4021 catcgaggct tactttctcg gcgggttcat tgtggccaac caggaacagt ggcaccatgc
     4081 ccacggggta gtgaaggctc tccgcgagga gctgcccaag agacccggct tcccctgcgt
     4141 gctcatgctt tgcgggaatc gcgagaagga ggcgctggag atcctgaggg aagggacccg
     4201 cgacctcccg gggcggattg aaatctacgg ggcggaaaaa gtttatgaaa ccgaatttct
     4261 ggccaaaaga gtgaaagccc tgatcatgga gtaccgccga gaccggggga taaaggaggg
     4321 ttagagacat gcattttgaa gagcggacga tcaagatcat catcgatgag gaaaaatgcg
     4381 tgggctgcaa aacccatgtg tgcgcgcagg catgtaagac ctacgatcgg ggcatcttgg
     4441 tgattaagga cggcaaaccc gccctcggcg gggattcgga attcggcaag cgggttggca
     4501 cggagtgcct cgcctgtgag tatgaatgct ggttccgggg gaacaaggcg atcaccatcg
     4561 aggcgccgat tcccgggctg gatgagtaca agaaaaaata cggagtcccc taaaagagaa
     4621 ctttcccaaa ggcaggtgaa aaaatggcga ttctactcaa gaaggatacc accatcctcg
     4681 tgcaagggat taccggaaga gaaggctccg cccgggccgc ctttatgaaa gggtatggaa
     4741 cgaaggttct ggccggagtc acgcccggga gggggggaga ggaggtctcc gggatccccg
     4801 tcttcaacac ggttgcggag gcgatccgga aagtgggtcc gatcgatgcc agcgtcacct
     4861 tcgttcccgg gaccggcctg aaggaagccg tcctagaggc gatcgattcc gggatcaaat
     4921 ttatcgtgag cccggccgaa cgggtccccc tgtacgacat tctggagatg gtggcttacg
     4981 cgaagcagaa aggggtccgt ctgctgggtc ctggttgcat cggggtcctt agcccgggaa
     5041 tcgcagccgc cggctggctc ggcggcaacc cggagtatgc ccgaagagtg tttctgccgg
     5101 gacccgtggg ggtcatgtcc cggagcggtg ggcagtcggg caccgttccc tgggccgtta
     5161 aggaagtagg cctcggcgtc agcaccgtgg tccacatcgg cacggagccg gtcttgggca
     5221 cctccttcgg ggacattctg ccccttttcg agaaggaccc ggacaccaag ggcgtggccg
     5281 tcttcggaga gatcggcggc ccctacgagg aggaggcggc cgagtgcgtg cggaaaaggg
     5341 aatacaccaa gcccctggtg atcttcgtcg ccggcgcctg ggcgcccgaa ggcatgcgtt
     5401 tctcccacgc cagcagcatc attgaaaggg ggaagggttc ggcgaagagc aaaatccaat
     5461 cgctcaagga tgccggggta cacgtggtgg accggcccga cgagcttgct ccgaccatca
     5521 agcggttaat ccaatcccaa taggaggtag aaagatgcct gtaatgagat ccgtgtttta
     5581 cattccggga aacaacgaca agatgatcgc caaggctcca acgacgccag ccgacatcat
     5641 caccctcgac ctggaagatt ccgtccctcc cgccgagaaa cccaaggccc gggacatggt
     5701 gcgggaaaat ctgaagctcg ccggaacggg cggctccacg gtctacgtcc ggatcaacaa
     5761 ctgggagacc ctcatgacca acgacgacct cgaagccatc gtccatgagg gactttccgg
     5821 tgtgtgcctg gccaaaaccg gcggtccaga ccacgtgcaa cgcctcgaat ggaagctgga
     5881 ggaactggag cgaaggagag gcttgccggt gggaaagatc gccatccagc tcctgatcga
     5941 gacggccaag ggggttatca atgcctaccc ctcggcgatc gccagcaagc gggtgaactc
     6001 gctcatcttt ggggcggtcg attataccaa ggacatgcgg gtgaagctca ccagcgaagg
     6061 agttgagcaa ttctacgccc gttcgcacac ggcggtcgcc gcccgggcgt caggctgcat
     6121 cgccatcgac tgccccttcg tggatttcaa aaatatcgag gcctttgaaa agagcacccg
     6181 cgagggacgg caaatgggat acgagggacg aatgctgatc catcccaccc agatcgagcc
     6241 ctccaaccgg atctattctc ccgcgcccga ggatgtggaa tgggccaagg gcgtggtcaa
     6301 ggtcttcgag gaagagggta ttgccaaagg aatggcggcc gtgtcctaca agggtaagat
     6361 ggtggacact ccggtctacg ataatgcccg gacgatcctg gcgacgttgg ccgagatcga
     6421 ggcctttgac aagaagcgca agcaggccta gaggccgcgg gattcaactt accggtcccg
     6481 cgttggggaa cgctttcggc acggctcctc ctctcctcca gggggggagg agcggttcag
     6541 attgagggga cggtcccctt cgtccccttc tcattccgcc gggcattaga ggggaggaat
     6601 gtcatgggcc agcgatagcc gaatttactt ccttcccttt ccccgttttt cggcgatgtc
     6661 ccttcacggt ccccctgatt ctccacaacc ttgaggattc atcatctcac ctggaaagag
     6721 accgctcggg accgggagga gaacggagag agaaataaat ggagaatgtg tcctacctca
     6781 cccccgaaat tcaggccctc ttggggcaag aggtgacttg gaaagcgacc gagccggtgg
     6841 atgccggcaa aatccaccgt tttgcgaaat ctttaggctt ggccaatccc atttattatg
     6901 atatcgaaaa gggccagccg gttgccccgc caacgttcct tttctctgtg aatcacgatt
     6961 ctttgggaga tttcgatgag accggaaggc cgacgaaaag gctatccctg cctccccctt
     7021 ttggtccggc catgcgaggg gggaacaaat atcaattctt ccagcaggtc cgtgtcgggg
     7081 accggctttg gatgaaaagg aaagtaaatg gcttggaaga aaaacagggc cgaaccggag
     7141 ctcttctttt cgtaccctat gatgtgcaat ataccaacaa gggcggcgac cttttgggca
     7201 tcaacacgga aaccctaatc ttccgcgtca cccaggaggg caaagagaag aatagaatga
     7261 gcagccacac ggtttttgaa gaccgcagag aaggccagga aatccccccg taccgggtca
     7321 ccgtgtctaa ggtccagatg atgatgtatg ccgctgccac ctggaatcct aaccagcttc
     7381 attgggactc tgatttttgc aagaaaaggg gctttgccga ggccaatatc gccggaccca
     7441 tgttcggtga ttatctggcg gaaatgctcg tgaaatgggt gggtgatcct tctcgcctca
     7501 aaagcctcga ttatgtcaat cggaacatgg cttttcccgg tgataccctg atctgccggg
     7561 ggaaagcctt gggcagaccc cgacaaggaa atacggaact gatcgattgc caggtatggg
     7621 tggaaaatca gaagggagag attctcgtgc aaggctcagg ccaggtgagc cttggataaa
     7681 ggcccttggc caaaggacgg aaaaaattct ccttcctctt ctctgcggtt cctaggtctt
     7741 ttccgcgtta tgataacccg gccttttgag atcctcccga ttagaccagg ctttctaata
     7801 cgaacaaaga aaatattgtg gtagtctttg cgagcccggg aggcgcggca atctcgtttt
     7861 tgcttgagat tgctttgtcg caacgttcct cgcgctaacg acttacttat ttcgtttgta
     7921 ttaattttac aatttttcgt gatttcatcc aaaatacctt gattacctct tccgattgtg
     7981 ataatttgca tttacttttg cgaatcgaat cctaaacaat ttcctttgag gaccttctat
     8041 gcttgatgaa ttccatcccc ctgctcgcct gcttatgggc cctggaccca gcaatgtgga
     8101 cccccgcgtg aacctggcca tggccaaacc cttgctcggc cacctggacc catacttcat
     8161 ccagatcatg caccaggtcc aggctctact gcgggaagtc tttgcgactc gcaattccct
     8221 caccatcccc atctccggga ccggaacggc cgggatggag gccgcctttg tcaacgtcgt
     8281 tgaacccgga gacaagatcc tgattggcgt gaacggcttc ttcggcgaac gcatggtgga
     8341 gattgccagt cgctgcgggg ttgaggtaaa gaagctcgac ggtacctggg gcaagccttt
     8401 tgagcctgcc gaggtggaga aagaactccg ggccttccgc cccaaggtcc tggcattggt
     8461 acacgccgaa acctccactg gtgtccttca accgctcgaa gaattctcct cgatcctgaa
     8521 aaaattcccg gagactctat tcctggccga tacggtcacc tccctaggcg gccatccggt
     8581 gaaagtggac gaatggggaa tcgatgtctg ctacagcgga acccaaaaat gtctgagctg
     8641 tccccccggt ttggctccga tcactttttc gccgaaggcc gtggaaagaa taaaaaaccg
     8701 gacccggaag gttcagagct tttatctgga tatggataca ctcggaaagt attgggggag
     8761 tgaaatcacc taccatcata ccgcccccgt cagcatgaac tatgccctgc tggaagccct
     8821 gcggatcgtc caggaagagg gcctggaccg gcgtttcgaa aggcaccggc gcaatcatct
     8881 gggtttggtg gccggaatcg aagccatggg gctaaagatg tttgttgacc cgccgcaccg
     8941 cctgtggtcc ctgaacacca tcgccatccc cgagggaatc gacgacggga aggtacgcaa
     9001 aagcctcctg gaggaatacg gcctggagat cggcggcggg ctgggtccct taaagggcaa
     9061 aatctggcgg gtggggttaa tggggtacaa ctccgatgag aagaatgtcc tctttttcct
     9121 caccaccctg gagcaggccc tgcgggcgca ggggttcgag gtacccccgg gtgtgggaac
     9181 ctccgcagcc cgggattttt atgctaaaaa taaataggca ggaccgagcc acaaattcgc
     9241 tgctcgctgc tggttcctag cttctgattc ctgttttcgc ctttaactcg caaggagttg
     9301 cttactgttc atcgctcagt acgatatttt atgggccaac cctggacctt tctttattct
     9361 aaaatcctct atacctgccg tatcttttcc ctgaaaaagg aaggctaccg ttccccgcgc
     9421 acgggcagag agcatgattt cttccttctt gaatccactg actgggtgaa cgtcattccc
     9481 cgaaccgcag acggaaggat catcctagtc aaacagtacc gtataggaac aaaagatttt
     9541 tctctggaaa ttcccggagg aatgctcgat gagggggatg atccggctgg ggcagcggct
     9601 cgcgaactct tagaggaaac cggctacgca ggcgaggaac cgatccttct gggcgtagtc
     9661 catccgaacc cggccatcca aactaaccgc tgttatactt ttttgattaa taatgtcgtc
     9721 ttcaaaaccc ctccccaaca ggattccacc gaagatatcg aggtccaatt cgcgtccctc
     9781 gcgcatatcc ccgagctcat ccggaaaggg aaaatcaccc atgccctggt gatcgccgct
     9841 ttctattggt acttctcgag tacagcctct aaggattctg gggctcaagg attcgcgtga
     9901 aaacattttt ggtattagga tttcccttga ctccgtgaat cctatgtttc cattcaggag
     9961 gggttaccag tcagcccata attgtatgga cgctgaagac taggccgttc gtgctgagct
    10021 tgtcgaagca tgagcctgtc gaagcatgaa cgatgtagcg atgcaccctt cgacaagcat
    10081 gccctgagtg agccgttcgc ccttcgacaa gcgttcgact gagctcacgc cgaaggctca
    10141 gggtgaacgg tggcaggcta aacggggaga ataataaaag ggaggagggg ttcaccatgg
    10201 atgttccttt aatgtctttg attgctttgt tcattgcgat cgtgattagc tgtacgacct
    10261 ctttgaacat cggcaccctg gcaatcgggt tatccctgat cgttggctac tattttggag
    10321 gggtcaagct tgcggaaatt gtcaagggtt atcccaccag cctgttcatc atgctggccg
    10381 gggtcaccta cctctttgcc attgctcaaa cgaatgggac cctggaaaaa atatccaagt
    10441 atgcgattaa agcggttcgc ggcaatgtag ccctgctgcc gattgtactc tttttcttag
    10501 cctttgccct ctcctccatg ggccccggcc aaataaccat ttcggcgctg ctggcggctc
    10561 ctgcaatgct cctggctcag gaggtgggga tttctcctct gctcatggct ttggtcgtgg
    10621 gcaacggcgc tcaagcaggg gccatgtccc ccatcgcccc tccggggatc atctccgcgt
    10681 ccatcctatc caaaatgggc atcaccggcg tcagcatggt cctctggatg aacatgctga
    10741 ttacccatgt ggcggtctct gtcctcgcct actttctgtt tggggggtta aagctcctcc
    10801 gtataaaaga taccggccag agggaaacgc tgaggaacct cccggttgaa ccctttacga
    10861 tggaacaggt ctgcaccatc atcggggtgg tgatcctgat cctcggcgct cttttcttca
    10921 agctggatat tggcatggga gcttttatga ttggtgcggt cctctccctg atcaaagctg
    10981 tggatgaggg gaaagccatc aaggccatgc cctgggggac catcctcatg gttaccgggg
    11041 ttaccgtcct ggtccagtta atgagccagg tgggcggcat ggacctcttt gccacgatca
    11101 tggccaaaat atctaccgcc tataccgtta cgatggtcgc aggcttcgta gcgggggttg
    11161 tttccgctta tgcgagcacg acaggggtca tactgcccgc gtttctcccc ctggcgcccg
    11221 gcttgctggc caaggttggc ggcaccgacc tgatggccct catatcgtcc atcgtcgttt
    11281 gcggtttcgt ggtggaccta agcccccttt ccaccaccgg cgccgtcttt attgccaatg
    11341 ctgcccccca ggcggataag aataaacttt ttcgtaacat gctcatctgg gggttgtcca
    11401 tgtcggtcgt cggggcggtg ataagctggc tggccttcac cgtcctgggt ctgccctaag
    11461 gaaaaagtgg gcctttgtct ccttttcatt ttttcagtag gtggacatga taaccacccg
    11521 ctggatttcg ttcgtccctt cgtagatctg ggtgatcttg gcgtcgcgca tgtagcgctc
    11581 cacggggtat tccttgacgt acccatatcc tccgaggacc tggaccgcct ccaccgtgac
    11641 ccgcatggcc gtgtccgcgc agaagcactt ggacatggag gagaggcgga tggtctccgg
    11701 gctgatgcgg gacatgtcct tgggaagctc ctggagtagg gatgcggtct tgtagagaag
    11761 ctggcgggcc gcctcgatgt tcatggccat gtccgccagc ttgaaggaga ttccctgctg
    11821 ggcgacgacg ggcttgccga aagcaatccg ttctttggcg taattaaggg agtactcaaa
    11881 agcgccggcg gcaattccga gggcctgggc gcccacggcc ggacggctga aatccagggt
    11941 cttcatcatg atggcaaacc ccattccttc cccccctagc aggttcccgg cgggaaccgg
    12001 gcagtcctcg aaaatcagtt ccactgtatc tgaagcccgg atgcccagct tgtcctcatg
    12061 tttccccacg gaaaacccct tggttccctt ttccaccacg aagacgctgg agcccctatg
    12121 cccgcccttc gccgggtcgg tcagggcgta gacagcgacc gtgtcggcca ccccgccgtt
    12181 ggtaatgaaa gtcttggtcc cgttaaggat gtaatagtcg cctttcttta ccgctttact
    12241 gcggaaggcg gccacgtccg aaccgccgct cgcttcggtc agggcaaagg ccgacaattt
    12301 ctcccccgaa gccagagccg gcaggaactt ctttttctga tccggattgg ccgccaggag
    12361 aatgggcaaa gttcccaatt cctgggccac cgggagcatg ccggtggagg cctccgcctt
    12421 ggataactcc tcgcagacaa tgaccagcgc taagagaccg gcccctgctc cgccgtgctc
    12481 ttccggaaag tcgaccccga aaaggccgtt ttcccggagc agctccacca tctcccagct
    12541 gaattccccc ttctggtccc gggccccggc ccccggctca actttctcct tggccagctt
    12601 cctcaccgta tcgcggagta atacttgctc ttcagtcagt ccgtactgca tgaccatctc
    12661 ctttccccac ccccttcctg ccttcctcgg agctccgaag aattccctta aatcaccccc
    12721 accctcaccc tcccccatcg agggggaggg aatttttatc tatttcaatt tttttaccct
    12781 ctccccttgc gggagagggc agggtgaggg gggttggggt ggagggggtt ccacatattt
    12841 tttaaaaaaa gagatttcta caccaaggta cttctgggag ggattgtaga aaaatccgtt
    12901 tagaaatgtc cataaaaaaa tggaggcacc tccggggagg agggctcctc cccggaaata
    12961 ggttgagcga tcgagcgcga tgcggcctag agcttgagac ccaaggggtt gtcgtagatc
    13021 gtccaggcca ggaagtagat gaacaggacc accgcccaga aaacattcag gg
//
LOCUS       DASPRR010000263         6520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_707307, whole genome shotgun sequence.
ACCESSION   DASPRR010000263 DASPRR010000000
VERSION     DASPRR010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707307"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /gene="porA"
                     /locus_tag="VEL68_10220"
     CDS             <1..252
                     /gene="porA"
                     /locus_tag="VEL68_10220"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HXZ36383.1"
                     /translation="AKILNKAKKVAVLDRDNSLGAGGIVAQEVKAALYGAVKKPRVFE
                     FIAGLGGKDITPEDIGEIFDYTMSHPSPNGAPVWVGVKE"
     gene            249..1133
                     /locus_tag="VEL68_10225"
     CDS             249..1133
                     /locus_tag="VEL68_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ36384.1"
                     /translation="MKGIETKAKELLNESLYSGHGACPGCAMPIILRSVFHALGEKTI
                     AAITAGCFGTISGTFPASPIKIPAYNTPFPSAGAAGSGIRAALDVKGDTETTVVAIAG
                     DGGTFDIGIQAMSAAAERNDDYIFICYDNEAYMNTGIQRSSATPWGAWTTTTPPSELK
                     VQPKKDIVQIMAAHHIPYVATASIAYPDDFVRKIKKAQETRGFKFIYAFSPCPVGWRF
                     PSEITIRLARLAVQTRVFPLYEVENGERYILNVEPEGIPVSEYLKPQGRFNFLREGDL
                     ERIQENVDREWERLMRRF"
     gene            1332..2177
                     /locus_tag="VEL68_10230"
     CDS             1332..2177
                     /locus_tag="VEL68_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36385.1"
                     /translation="MSRWRALFWGTFLSSLTLAGLPLGAEERTVGEGEHRGFILHEGE
                     QVLRVEPIRPGQTIQAQVYPLWDVEKGARVEWVLTDGGGTKLRTGSHRQPEEHPLLLE
                     WTSNSEPRPDSYRIQIQGSGGHFPAEVLGQYVVQISLWDQNDGDSGTDAPETFEKALE
                     LPVSEPGVHNFVECFASGTADIYDIYKISVRPNHSLNLKATPVLWHGVDKKGKVYWEF
                     LNRSFKRMKDGQSSLSETSSFMVKVFHPQVRSGNKPAIFYLLVRIEGDVSLIYSLQTE
                     VKEGQ"
     gene            2174..2593
                     /locus_tag="VEL68_10235"
     CDS             2174..2593
                     /locus_tag="VEL68_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXZ36386.1"
                     /translation="MRMEKLLFKKFGTSFPAGTVLFEEGQPCSGMYIIQKGRVRLYKK
                     AGQKKVTIDILEDGDFFGEMACLLGQPRSINAVVEEESQILVVESEVLDELFREKSGL
                     GIKVLGNLASRLRKAYEIIGRLVEEQGQPREKEPLPP"
     gene            2590..2991
                     /locus_tag="VEL68_10240"
     CDS             2590..2991
                     /locus_tag="VEL68_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXZ36387.1"
                     /translation="MSREEQLFWKFGKNFPKGTILCREGDAGHEMFIIQKGKVLVRKK
                     VGKGEKVLAELSDGEFIGEMALLLGMDRSATVEIVEDSKILVVGPDTFESLLKNSPEI
                     AMKMLKKMALRLRALDDRLETALAEHLPKVD"
     gene            complement(3340..3942)
                     /locus_tag="VEL68_10245"
     CDS             complement(3340..3942)
                     /locus_tag="VEL68_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36388.1"
                     /translation="MTKGQTVAEVKRRKEGDSAADAAAAMIRQSSKGGQLISEAEILG
                     RLGDQHLLPSPAQGHAGEVGTLLKKLVAESEDLKAVAAQDGARSYYSSDFMTDAYARI
                     LLQKQGDRLRLIAETVRENSALYPRPVPLDLFTKSPFGLTQQEILNELERMEGKEEYR
                     DIAPTTTSTSRIFLYSSLHLEPDHASMLAEWLDVGQSNNP"
     gene            complement(3954..6059)
                     /locus_tag="VEL68_10250"
     CDS             complement(3954..6059)
                     /locus_tag="VEL68_10250"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HXZ36389.1"
                     /translation="MVKYGGWAGKVLRVDLSTGRIWTEDTIEKYKDYLGGTGVGYKVM
                     WDEVSAGTKPHDPENRIIFAVGPLAGTGAPCNGRTAITTLWPTCWPIPLVASGHMGGQ
                     FAAKLKYAGYDGIIIQGKADRPVWLSIVDQQVEVQDARRLWGQGIRRTTEAIAQIMGT
                     EAVVAAIGPAGENLVPMSVVMNSVSHSAGGIGSVMGAKNLKAIGVQGTGSVRIAGDKE
                     EWEKLIKYHLSLLGANNQHVVPNAPQPWAEFYEPRSRWVGSQGHSWGANPRPQETGAC
                     DPHDLNRVAYRTNNAAYFMGEIAWQYTIRGNGCTSCPIRCHTILKVPALSTKYGIPEV
                     GQNTCSGLNFGRAFFKSFPDGPQGQTNVEACMVGMHLADDLGVWCNYSQMQRDFQKLY
                     YEGFIKKKLGEKEFKSYSWGKYEKGDPSFLFEIIPKIASKEGELGTALGLGTGYLLER
                     WSISEAYWGKDKDLAYWKMGHPKHHSTEDAGQCGLLINLMYNRDAQCHSHCNFVRNGL
                     PLAVQKRLAAEVWGSPDAVDAVGAYTPMNPSKAKMAKWSLLRKELHDSLSLCNWMGPW
                     VASPLKERGYRGDDSIESMLYSLATGDKQNRQELDRMAERIFVLHRALTIRDMGTKEM
                     RTKHDFMPEWVFTDKSGKAPFTKGTTHMDRNDIKVAIDMFYDEMGWDRATGAPTSQAY
                     RQVGLEKVAEALAKQKLLA"
     gene            complement(6119..>6520)
                     /locus_tag="VEL68_10255"
     CDS             complement(6119..>6520)
                     /locus_tag="VEL68_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HXZ36390.1"
                     /translation="RDFKGMFAQAKFYLISIFKGEGNPFRPSPEEKFNPLQKLAYGSI
                     MFFFAPILAITGLLCSDIQFLRKYILLWDIVGVVNALHVLAAYVFVLYLVVHLYMATL
                     GRTTFAHIKAMIVGYEEEFEEPGKEVGETAP"
BASE COUNT         1402 a         1836 c         1896 g         1386 t
ORIGIN      
        1 gccaaaatcc tgaacaaggc caagaaggtg gctgtcctgg accgggacaa ttcactggga
       61 gccgggggca tcgtcgctca ggaggtgaaa gcagcccttt atggagccgt gaagaaaccc
      121 agggtcttcg agtttatcgc cggcctgggc gggaaagaca tcaccccgga agacatcgga
      181 gaaatttttg attacacgat gagccatccc agccctaacg gggctccggt ttgggtggga
      241 gtcaaggaat gaaaggaatc gaaaccaaag ctaaagaatt gctgaatgag tccctttact
      301 cgggtcacgg tgcctgccca ggttgcgcca tgcccatcat tttacgcagc gtctttcatg
      361 ccctcggaga aaaaaccatc gccgccatca cggcaggctg ttttggaacc atatcgggaa
      421 ccttccccgc ttcgcccatc aaaattcccg cctataacac ccccttccca tctgcgggag
      481 cagcaggatc tggaattcgg gcggcgctgg acgtgaaagg ggacactgaa accacggtcg
      541 tcgccatagc cggagacgga ggtactttcg acatcggaat ccaggccatg tccgccgccg
      601 ccgaacggaa tgacgattac atcttcattt gctacgataa cgaagcgtac atgaacaccg
      661 gaatccagcg ctcctcggct actccctggg gcgcctggac cacaaccacc cctccttcag
      721 agctgaaggt tcagcccaaa aaagacatcg tccagatcat ggccgctcac cacatccctt
      781 acgtggccac ggcctccatt gcctatcccg acgacttcgt ccgcaagatc aagaaagccc
      841 aggaaacccg gggattcaaa ttcatttatg ctttctcgcc ttgtcccgtg ggctggcggt
      901 tcccttcaga gattaccatc cgcctggccc gtctggccgt gcagaccaga gtttttcccc
      961 tatacgaggt ggaaaacggc gaacgttata tcctcaatgt ggaaccggag ggaatcccgg
     1021 tgagcgaata cctcaaaccc caggggcgtt tcaatttcct ccgggaaggg gacctggaac
     1081 ggatccagga gaacgtagac cgcgaatggg aacggctgat gaggagattt taagcagtct
     1141 cggcccaaag aacttggaag agttggcccg gaagaccaga aagaaagtga cccgatgttg
     1201 aatatcggtg aggaagcgat cgtagggttt gaaaaggggg gcgggaatcg cctaaggaaa
     1261 aggtcttttt tgcttgctgc ggatgggttc aaatccggac tctttcgcgg gtctatgagg
     1321 ttcatttcgc gatgtccagg tggagagcgc tcttctgggg gaccttctta tccagcttga
     1381 ccctggcagg tcttcccctg ggggcagagg agagaaccgt aggagagggg gagcatcggg
     1441 gctttatcct gcacgaggga gagcaagtcc tgagagttga gcccatcagg ccgggtcaga
     1501 ctatccaagc ccaggtatac cccctgtggg atgtggaaaa aggggctcgc gttgaatggg
     1561 tgctgaccga tggaggagga actaagctcc ggacgggcag ccaccgccag ccggaggagc
     1621 accccctcct cctggaatgg acctccaact cggaaccccg gccagacagc taccggattc
     1681 aaatccaggg cagcgggggt catttccccg cagaagttct gggccagtac gtcgtccaaa
     1741 tctctctctg ggatcagaac gacggggatt cgggaaccga tgctccggag acctttgaaa
     1801 aggccctgga gctaccggta tctgagccgg gggtccacaa ttttgtcgag tgtttcgcca
     1861 gcggaaccgc tgatatctac gacatctata agatctccgt ccgacctaat cattctctga
     1921 acctaaaggc cactccagtc ctttggcacg gagtggataa aaagggaaag gtctactggg
     1981 agttcttgaa ccggtctttc aaacggatga aggacggcca aagttctctg tcggagacat
     2041 cctcatttat ggtaaaagtc tttcatcccc aggtgcgatc ggggaacaaa ccggccatct
     2101 tttaccttct ggtcagaatc gagggagacg tctccctgat ctattcgctt cagacggaag
     2161 tcaaggaggg ccaatgagaa tggagaagct tctctttaag aaattcggca cgagctttcc
     2221 tgccggaacc gttctctttg aagagggcca gccctgttcg gggatgtaca tcatccagaa
     2281 gggccgggtc cggttgtaca agaaggccgg ccagaaaaag gtcaccatcg acatcctgga
     2341 agacggagat ttcttcgggg aaatggcctg cctgctcggc cagccccgct cgatcaacgc
     2401 agtggtggag gaagagagcc agattctggt cgtggaatcc gaggtcctgg atgagctttt
     2461 cagggaaaag agcggcctgg ggatcaaggt tttgggaaat ctggcgtccc ggttgcgaaa
     2521 agcctatgaa attatcggcc ggctggtcga agagcaaggc cagccccggg aaaaggagcc
     2581 cctgccgcca tgagtcggga agagcagctc ttctggaaat tcggtaagaa tttccccaaa
     2641 ggaacgattc tctgccgcga gggagatgcc ggccacgaaa tgttcatcat ccaaaaggga
     2701 aaagtcctgg taaggaagaa agtgggaaaa ggggaaaaag tcctggccga gctctccgac
     2761 ggagaattca tcggggagat ggccctgctc ctgggaatgg atcgctccgc gacggtggaa
     2821 atcgttgaag actccaagat tcttgtggtc gggcccgata ccttcgaaag tctgctgaag
     2881 aatagtcctg aaatcgccat gaaaatgttg aagaagatgg ccctgaggct ccgcgccctg
     2941 gacgatagac tcgaaaccgc tctggccgaa cacctcccca aagtagacta accctcctat
     3001 ttcgagtgaa tcttcatccc ccgtccgaaa agccgatctc caactaattg ccttaaggaa
     3061 aaccccctgg ctccgcctgg ggagtcataa aatttgacaa ttctgggtgc gtgggtcatg
     3121 gcccgggggc aaggccgtta cgcgcagaaa gcatcggcct gcccattttg gatttcaaat
     3181 tgcggatttt ggattttggg gttctctttt tcaatttgca ttccgcaatc aacaattcgc
     3241 aattttgaag gccccccgga agtccaacct ctcctttttg aggggcatct gccccagcga
     3301 attccatatc ttcaaaggga caggaggagc caataacctt caggggttgt tggattggcc
     3361 tacgtcgagc cattcggcga gcatggaggc atggtcgggc tcaagatgga ggctggaata
     3421 caggaaaatc ctggaggttg atgtcgtggt gggtgcgata tcgcggtact cttctttccc
     3481 ttccatccgt tccagttcgt tcaggatttc ctgttgcgta aggccgaaag gggatttggt
     3541 gaagaggtcc aaaggaacgg ggcgagggta cagggcggag ttttcgcgga cggtctcggc
     3601 gatgagacgc agccgatcgc cctgtttctg gaggagaatc cgggcgtaag catcggtcat
     3661 gaaatccgag gaataatagc tccgggcgcc atcctgggct gcgaccgcct tcaaatcctc
     3721 gctttctgcg actaatttct taaggagggt tccaacctct ccggcatgac cttgcgcagg
     3781 agagggtaaa agatgttgat cgcccaaccg cccgagaatc tctgcctcgg agatgagttg
     3841 gcctcccttg ctgctctgcc ggatcatcgc ggcggcagca tcggctgcgg aatcgccttc
     3901 ctttctcctc ttgacctctg ccacggtttg ccccttcgtc atcgccgaac ccgtcaggcc
     3961 agaagcttct gcttagccag cgcctcagcg accttttcca gaccgacctg gcggtaagct
     4021 tgagaggtgg gcgcccccgt agcccggtcc caacccatct cgtcgtaaaa catgtcgatg
     4081 gccactttga tgtcgttccg gtccatgtgc gtggtcccct tggtgaaggg agccttgccc
     4141 gatttatcgg tgaagaccca ttccggcatg aaatcgtgct tggtacgcat ctctttggtt
     4201 cccatgtccc ggatcgtaag agcgcgatgg agcacgaaga tgcgttccgc catccggtcc
     4261 agttcctgcc tgttctgctt atcgccggtg gccaggctgt agagcatgga ctcgatggaa
     4321 tcatcccccc ggtagccccg ttccttgagg ggagaagcta cccagggccc catccagttg
     4381 cagagggaga gcgaatcatg gagttcttta cgcaacaggg accacttggc catcttggcc
     4441 ttggaagggt tcatcggggt gtaggccccc actgcgtcca cggcatcggg ggacccccag
     4501 acctcggccg ccaggcgttt ctgcaccgcg aggggcaggc cattccgcac gaaattgcag
     4561 tgggagtgac actgggcgtc gcggttgtac attaaattaa tgaggaggcc gcactggccc
     4621 gcgtcctcgg tggagtggtg cttcggatgg cccatcttcc agtaggctag gtctttatct
     4681 ttcccccagt acgcttccga gatggaccac cgttcgagga gatatcccgt tcccaggccg
     4741 agggcggtcc ccagctcccc ctctttggag gcgatcttcg ggataatctc gaagagaaag
     4801 gagggatctc ccttctcgta tttgccccag gagtagctct tgaattcctt ctctcccagc
     4861 ttcttcttga taaatccctc gtagtaaagc ttctgaaagt cccgctgcat ctggctgtag
     4921 ttgcaccaga cccccaggtc atcggccagg tgcatgccga ccatgcaggc ctccacgttc
     4981 gtctggccct gggggccgtc ggggaaggac ttgaagaaag cgcggccgaa gttgaggccg
     5041 ctgcaggtgt tctgccctac ctccgggatg ccgtatttcg tggacagggc ggggaccttc
     5101 aagatcgtgt ggcaccggat gggacaactg gtgcagccgt ttccccggat ggtgtactgc
     5161 caggcgatct cccccatgaa gtaagcggca ttattcgtcc ggtaggcgac ccggttcaga
     5221 tcatgggggt cgcacgctcc cgtttcctgc ggacgcgggt tagcgcccca gctgtgcccc
     5281 tgggagccga cccatcggct tctgggctcg taaaactcgg cccagggctg gggggcgttg
     5341 ggcacgacgt gctggttgtt cgcccccagg agggagagat gatacttgat cagcttctcc
     5401 cactcctctt tgtcgcccgc gatccggacg ctgccggtgc cctgtacgcc gatggctttg
     5461 aggtttttcg cccccatcac cgaaccgatt cctccggcgg agtgggagac ggagttcatg
     5521 accacggaca tggggaccag gttttccccc gccgggccga tggccgccac gaccgcttcg
     5581 gtccccatga tctgggcgat cgcctcggtc gtccggcgga ttccctgccc ccataagcgc
     5641 cgggcatcct ggacctccac ctgctggtcg acgatcgaca gccagacagg acggtcggct
     5701 tttccctgga tgatgatccc atcgtatccc gcatacttga gcttggcggc aaactgtccg
     5761 cccatgtgcc cgctggcgac aaggggaatc ggccaacagg tcggccacag ggtagtgatg
     5821 gccgtgcggc cgttgcaggg ggcgccggtt ccggccaggg ggcccaccgc gaagataatc
     5881 cggttctcgg ggtcgtgggg cttggtcccc gccgagacct cgtcccacat cactttgtat
     5941 ccgactccgg ttccgcccag gtagtccttg tacttttcta tggtgtcctc ggtccagatg
     6001 cgcccggtcg aaagatcgac tcgcagcacc tttccggccc atcctccgta tttaaccatg
     6061 gtctatcctt ccgtggacaa aagaatgaaa gtgcaagtga aagtgaaagg cggaatcgtt
     6121 acggcgccgt ttctccgact tccttcccag gctcctcgaa ctcttcctca tagccaacga
     6181 tcatggcctt gatatgcgca aatgtcgtcc gtccgagggt cgccatgtag agatggacca
     6241 ccaggtaaag gacgaacacg tatgcggcca ggacatgcag ggcattcact acccccacga
     6301 tgtcccagag cagaatatat ttgcgcaaga attggatatc gctgcagagg aggcccgtga
     6361 tcgccaggat tggggcgaag aaaaacatga tcgacccata agccagcttc tgcaaaggat
     6421 tgaatttctc ttcgggtgac gggcggaagg ggttcccctc tcccttgaaa atcgagatca
     6481 gatagaactt cgcctgagcg aacattccct tgaagtcccg
//
LOCUS       DASPRR010000264        17351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2155065, whole genome shotgun sequence.
ACCESSION   DASPRR010000264 DASPRR010000000
VERSION     DASPRR010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 17351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17351)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17351
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155065"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VEL68_10260"
     CDS             complement(<1..1013)
                     /locus_tag="VEL68_10260"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HXZ36391.1"
                     /translation="MTEILITHADPKKLKKKPDPDAQLGFGKIFTDYLFMMDYRKGEG
                     WKNPRIEPYRPIPMDPAALVLHYGQEVFEGLKAYRWANGDLYLFRPRMNYERMNRSAR
                     RVCLPEIDVDLAIEATRQLVRLERDWVPSSPGASLYIRPNMIATEPALGVQIAKEVLF
                     YIMVGPVGAYYPQGFNPVDIYLEEKYIRAARGGLGEAKTLANYAASLLAQEEAHEKGF
                     TQVLWLDAIERKYLEEVGTSNIFLYIGDEVVTPPLGGTILPGITRNSVLHICRNWEMK
                     VSERMVSLDEVLSGLESGRVKEIFAAGTAAVISPVGKVAYKGKNYVINQGKVGPLARK
                     LYDT"
     gene            complement(1172..1639)
                     /locus_tag="VEL68_10265"
     CDS             complement(1172..1639)
                     /locus_tag="VEL68_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXZ36392.1"
                     /translation="MEAISRLKYGGLTRLAKPLGNLLAEYQDPDFELAENDWVIPVPL
                     HTRRLRERGFNQSLLLARQVSRRRSIPLNFTALHRARQTQPQTQLSGAERRKNVRGAF
                     EVKKREAVKGKRILLIDDVFTTGATVQECAYVLREAGATEVHVLTLARVGWGY"
     gene            complement(1906..3261)
                     /locus_tag="VEL68_10270"
     CDS             complement(1906..3261)
                     /locus_tag="VEL68_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ36393.1"
                     /translation="MFSRTHFIVFLLVFIAFGYLFYLNPGEVEFTLWQDLHLIISPAL
                     IAFGAFIIGAFFVFLVTLFVDARRAFQLWRSSKTDRKDGKVRERYSDALEEMLKGNIP
                     RAKETLSKIIDTRPNHLPAHISLANLYSLEGKYSEAIDTLTKAKAIEPESLELLLDLV
                     KNNVGLRRYAVALEVLDTILDRDPSNREALRKKRDIHIITGNWDEAYHTQKSVMKHTR
                     EKEYALAEKRFLSGMEYKFAEELAQKGEFKDAEKALREVLKEDSTFFPAHVSLGDVLQ
                     RQGSFEEASQTWKKALEASWNPVFLERLESLYLSMANPQKALDLYRDYMRQRPEDSVL
                     RFFFSRLLIRLEMVDEAIEQLRDLETAGVLFSELFVLLGQAYHRRGEFVQSMESYEKA
                     LDTKKFPLPAYQCSSCGRTQAEWSGFCEACHSWGSYSMHLPEFSQKMPVIPFYHFPAR
                     I"
     gene            complement(3271..3753)
                     /locus_tag="VEL68_10275"
     CDS             complement(3271..3753)
                     /locus_tag="VEL68_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXZ36394.1"
                     /translation="MKKFRLVLLFVGRASDSYIREGCRIYEERIRRYAELRLVPIPEE
                     RIAPKGRKEYILRQEGRRIRENLPPEAFTVALDEKGKPLTSEAFSRNLELWGQWGSRE
                     IVFILGGPYGLEESLKEKVDFRLSFSTMTLAHGMARMLLLEQVYRAFSLLRGEPYHKS
                     "
     gene            complement(3750..4133)
                     /gene="rsfS"
                     /locus_tag="VEL68_10280"
     CDS             complement(3750..4133)
                     /gene="rsfS"
                     /locus_tag="VEL68_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXZ36395.1"
                     /translation="MIPSSSQEKAFLCARALLDRKAIDLVILEVKDLSSFTDYFLICS
                     GNSDRQVQAIASHIEEKLGKEGIHPLGIEGKREGRWTLLDYGDVVVHIFFQPVREFYD
                     LERLWSEATRVELPLSKKVRPKRSR"
     gene            complement(4130..4801)
                     /gene="nadD"
                     /locus_tag="VEL68_10285"
     CDS             complement(4130..4801)
                     /gene="nadD"
                     /locus_tag="VEL68_10285"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXZ36396.1"
                     /translation="MKKVGLFGGTFNPIHLGHLRAAEEVSHSFDLHRVFFIPSSYPPH
                     KKKEGILDATSRAEMVRRAIADNPRFAFSEVELERPGKSYSIQTIEHFRHQSGPGTQV
                     YFIVGLDAFLEVSTWKEYGALFRLCHFVVMTRPGFEKIFSREHLPVELTNEFCYDTEK
                     GGYAHKSGFCVYPAEVTGMDISSSRIRQWIREGRSIKYLVPPAVEAFILSNKLYQNKD
                     VETAG"
     gene            complement(4798..6054)
                     /locus_tag="VEL68_10290"
     CDS             complement(4798..6054)
                     /locus_tag="VEL68_10290"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXZ36397.1"
                     /translation="MSLRDEMVMIGQRAREASYALGNLSTSVKNSALEGMAEALLFSA
                     PGLKQANVLDLKAAKGEGLSSAMIDRLTLSDAVLMRMAASLKEIALLPDPVGEVNRMW
                     TRPNGLTVGKMRIPLGVIGIIYESRPDVTSDAGGLCLKAGNAVILRGGSEAIHSNLAI
                     AEVLRKVCREQGIPAEAIQVVPSTSRLAVEEMLQLEDYIDVIIPRGGEELIRFVVSKS
                     RIPVIKHYKGVCHVFVDASADLDMAQNISFNSKVQRPGVCNAMETLLVHRQIAPVFLP
                     PVAERFKAAGVELRGCPETRRILGGIGEASEEDWLAEYLDLILAVKIVDDLDQAIRHI
                     IRYGSSHTEAIVTQNYTNAQRFLREVPSSTVLVNASTRFSDGFQLGLGAEIGISTTKL
                     HAFGPMGVEDLTTSKFIVYGSGQVRT"
     gene            complement(6194..7390)
                     /gene="proB"
                     /locus_tag="VEL68_10295"
     CDS             complement(6194..7390)
                     /gene="proB"
                     /locus_tag="VEL68_10295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXZ36398.1"
                     /translation="MNRKSILEKVRRVVLKVGSRVLTGKGRTLSQPVFDRLAQEISAA
                     KKRGYEVVLVSSGAIAAGMGRLGLLEKPKMMPEKQASAAIGQSALMWSYEKAFSFYGE
                     KVAQVLLTRDDLSNRNRYLNARNTLLSLLDFGVIPIINENDTVVVQEIKFGDNDNLSA
                     LVTNLVNADLLVILSDIDGLYDKDPRVHKSARLIRSVHEVTEEMEQKATGTLSPFSIG
                     GMVTKLQAARKAALFGVPTVLANGMMEGALEKILRGEELGTLFTSQVNKLTSRKHWIA
                     FTLEPQGRIIVDEGAKRAILQKGRSLLPSGVLSAEGKFSLGDPVALSDVNGQEFAKGL
                     TNYDSSEINKIKGLKTSEVESRLGYKYSDEIVHRDDLVCVSPGYHQTPNLECQIPKKF
                     KNPKTQ"
     gene            complement(7387..8349)
                     /gene="obgE"
                     /locus_tag="VEL68_10300"
     CDS             complement(7387..8349)
                     /gene="obgE"
                     /locus_tag="VEL68_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXZ36399.1"
                     /translation="MSFRREKYVPRGGPDGGDGGKGGDVILKADDQLSTLLDFRYRQH
                     YHAKRGQHGRGKNQEGRSAPALLLPVPRGTIVRDGQSGKELRDLTGENETFVVARGGK
                     GGRGNARFASSTHQAPRHAEPGQPGESCWVLLELKLMADVGIIGYPNAGKSTLISKVS
                     SVRPKIADYPFTTLVPNLGVVSWRDHLPFVIADIPGIIEGAHRGAGLGLKFLRHVQRT
                     SLLLHVLDLSSLNPRDPLEDYRILNRELALFDPGLARKPQVVALNKIDDPESKKRLPA
                     VMAFFKRIKKPTYPISGLSGEGLPKLLGALTRHLDKGLKKERSN"
     gene            8254..8454
                     /locus_tag="VEL68_10305"
     CDS             8254..8454
                     /locus_tag="VEL68_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36400.1"
                     /translation="MVVGLQNHIPAFSPIAAIGTSAGNVFFTAKADAAVSSIAGLDEN
                     FGFIDELDRSDPRSSRAPAEPR"
     gene            complement(8464..8739)
                     /gene="rpmA"
                     /locus_tag="VEL68_10310"
     CDS             complement(8464..8739)
                     /gene="rpmA"
                     /locus_tag="VEL68_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXZ36401.1"
                     /translation="MAHKKGQGSSRNGRDSAGQRLGVKRFGGQRVHAGSILVRQKGTQ
                     IHPGKNVGVGKDFTLFAKIDGIVTFERLDRERKKVSVYPSQAEGAPA"
     gene            complement(8838..9152)
                     /gene="rplU"
                     /locus_tag="VEL68_10315"
     CDS             complement(8838..9152)
                     /gene="rplU"
                     /locus_tag="VEL68_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXZ36402.1"
                     /translation="MYAIIRTGGKQFKISPGDIIKIEKIAGQKGESVEMKDVLFYAEG
                     ENVLTGQPMLSNVKVVGEILGQHRAKKVIIFKKKRRKGYDKKTGHRQSFTSLRIKEIN
                     LQ"
     gene            complement(9232..10800)
                     /locus_tag="VEL68_10320"
     CDS             complement(9232..10800)
                     /locus_tag="VEL68_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXZ36403.1"
                     /translation="MSNEILINASSGETRVALLEGDVLVELYVERSSEQGIGGNIYKG
                     RVVRVLPGMQAAFVDIGLEKAAFLYVSDVHQDFDDLEILMRGREEDGNGHSPADEEEV
                     LNLDTPFHIEDLLHEGQEVLVQVSKEPLGTKGARITSHISLPGRHLVLMPMVDHIGVS
                     RRIENEGERKRLRDVIQQIRPPGCGLIVRTASEGKGEAELRQDLDFLLKMWANIQKRK
                     AVSPVPSLIHKDLDISLRAIRDLFTQEVDRVVVDSREEYQKIMEFCDTFMPQLKASIE
                     IFEKEEPLFDYFGIEMEISRALGRKIWLKSGGYIVIDLTEALTVIDVNTGRYVGKRNL
                     EDTILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDMEKESSREKVFQALTEALKKDKSK
                     TNILKISELGLVEMTRKRTRESLGRILSERCFYCEGRGFLKSRSTICQEILREIQRDI
                     RDLKEETILVHVHPNVANHLYDEARADLEKLEKRYKKRIAITGKNDFHLEQYEVQSHN
                     GHFAVDKPKSEILT"
     gene            complement(10778..13315)
                     /locus_tag="VEL68_10325"
     CDS             complement(10778..13315)
                     /locus_tag="VEL68_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HXZ36404.1"
                     /translation="MKAEDLLRIAKPSRYLGGETHSVIKDPRKVKLTFALAFPDVYEV
                     GMSHIGFHILYSLLNANPEIACERVFAPWPDFENLLRERGLPLCSLETSRPLKEFALL
                     GFSLQYELNYTGVLNILDLAGIPLRAQQRDENDPLVIGGGPCALNPEPLADFFDLFVL
                     GDGEEVVLELCQAIVASKEKGESKRTLLERLAGIEGVYIPSFFNLEYEDDGRLRQILP
                     IQSGYSHVTRRVLADLDKGPFPSRPILPLMEVIHDRLNLEIARGCTRGCRFCQAGMIY
                     RPLRERSPRKILELAKCGLQSTGYDEVSLLSLSTGDYSCIEPLLSSLLEHTREGRIAV
                     SLPSLRVETLTPTLIQQVREVRKTGFTLAPEAGTDRLRRVINKGNTEEDLRSTLHQVF
                     SAGWRLVKLYFMIGLPTEEEKDLQGIAYLCHEALKLARRAKGSAQINVSVSTFIPKAH
                     TPFQWEPQSSPEEVERKQRFLRKSLDRHGLQFKWSDSRLSLLEGVFARGDRRLSHLLE
                     TAYFLGCRLDGWGEHFRYDLWEEALRQSGVNPSFYLRKRDVDEILPWDHLHSRVSKSF
                     LKQEREKALGNLCTPDCRQASCNGCGVCYGVDGLSNRFAEWETVLNLAPAATRRTESS
                     LPVIRFRIRLTKLGPIRFLGHLDFSQGIIRAFRRAGIPLVFSQGFHPLPKIAFGPPLP
                     VGYESWAEYLDFHVWEKFQPGRALQRLNEVLPHGVEFLEIEEIPLSFPSIFDNMAYIL
                     YNIVFPDPVGVPTEKIAGFGKADSFWITWSRKKKDIDLKKFVESVTVVDERTVQIRML
                     SSTQGTLRPEEALGYLLGWAEEGKSGFSIKKIQVGFKNSGHVQRDPD"
     gene            13690..14133
                     /locus_tag="VEL68_10330"
     CDS             13690..14133
                     /locus_tag="VEL68_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36405.1"
                     /translation="MNLKAKVYVENQKKRVLEALNARLARLKEKGLKENIVQKDATVR
                     KLKAQLRKADHRLASIAAQEKLNQERVQAKLDRQAAEKAAKEAPRPEAEKGEPEKKEK
                     KAKKEKQEKPAKPEGKVEGEKEKKEKQEKPKKAPDKEEEQSEGSP"
     gene            14211..15173
                     /locus_tag="VEL68_10335"
     CDS             14211..15173
                     /locus_tag="VEL68_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXZ36406.1"
                     /translation="MSVLVTGGCGLIGSRLTRMLVEKGEKVHVLDIHLRPQCFSGISD
                     RIEFVRADVGNFAKVLEAVKECSPTVIFHLGGMLSLPSNADPQSSFSTNVAGTYHVLE
                     AARLFRVPKVIFTSTVATYGLDIQSKTINDSTLQRPITMYGSTKVFAELLGRFYRNKY
                     GVDFRAVRYPSVVGPGAKTAHISIYNAWAVEKAFFGEPYEVFVAPHVRCPIIYFKDAA
                     RSLLLLEAAPPEAIRTVCYLLAGIQPMISAEELVGEIKKHFPQALLTFKPDPLAMEVH
                     GKSQGVAYEDFNAQKEWGWKTEYTLPRMIGDFYAELKAHPEWYR"
     gene            complement(15318..15602)
                     /locus_tag="VEL68_10340"
     CDS             complement(15318..15602)
                     /locus_tag="VEL68_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXZ36407.1"
                     /translation="MTKSQVVAYLAEEVGITKKQGAAVLDALAGLAVKIIKKGDKLAL
                     PGFVTVKVQNRKARIGRNPQTGEPLKIPAKKVVKAVPAAALKGILGAKKK"
     gene            complement(15701..16342)
                     /gene="pal"
                     /locus_tag="VEL68_10345"
     CDS             complement(15701..16342)
                     /gene="pal"
                     /locus_tag="VEL68_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXZ36408.1"
                     /translation="MKGIRLLLIGLLILALGPGIGCAKKTIRSDSPVYTPSRVEPGPA
                     ETRPEAAAPDKTAALEKEKKLAEESLREESLREKALREKALQEEAARREAAAREAASK
                     TVSLEPVFFDFDQWSIREDQKEVMAKNSQWLKSNPNVKVRLEGHCDERGTAEYNLALG
                     QKRAEGVKSFLEGLGISNQRMATISYGEERPLDPGHNEEAWAKNRRVDIVPAK"
     gene            16492..17094
                     /locus_tag="VEL68_10350"
     CDS             16492..17094
                     /locus_tag="VEL68_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ36409.1"
                     /translation="MKVLGIHGSPRKGGNTEILLKEFLRGCKEGGAEVEEIFLRELKI
                     SPCLEIYACKKDGQCPIQDDMKALYPKLAEADVLAVASPIFFYAVSAHLKAMIDRCQA
                     LWAKKYLLNQPIAPGRSGRKGVFLAVGGSRGGKTFDGALLTMKYFFDALDMTFHRSLL
                     YREVDAKGEILQHPTAMAEAYALGKESVRKIAEFEMKSAK"
BASE COUNT         3858 a         4791 c         4761 g         3941 t
ORIGIN      
        1 gtatcgtaga gcttccgcgc cagaggacct acctttccct ggttgatcac gtaatttttc
       61 cctttgtagg ccacctttcc cacaggggaa atgaccgcgg ccgtcccggc ggcaaagatc
      121 tccttcaccc gtccggattc caaaccggat agcacctcgt ccagactgac catgcgctcg
      181 gagaccttca tttcccagtt ccggcagatg tggaggacgg aattccgagt tattccgggt
      241 aggatcgtac ctccgagagg aggggtgacc acttcgtcgc cgatgtacaa gaagatattg
      301 cttgtcccca cttcttccag gtatttccgc tcgatggcat ccagccaaag aacctgggta
      361 aaacctttct catgggcctc ttcctgggcc agaaggcttg cggcgtaatt ggccagagtc
      421 ttggcttccc ccaatcctcc ccgggcggcg cggatatatt tttcttcaag atagatatcc
      481 accgggttga atccctgtgg gtaataggcg cccaccggtc cgaccataat gtagaaaagc
      541 acctccttgg cgatctggac ccccagcgcg ggctccgtgg cgatcatgtt cggccggatg
      601 tagagggagg cgccgggaga tgatgggacc caatctctct ccaggcggac cagctgcctg
      661 gtcgcttcga tggccaaatc cacgtcgatc tcgggcaggc acacgcgcct ggcagaacgg
      721 ttcatccttt cgtagttcat ccgggggcgg aagaggtaaa ggtctccatt ggcccagcga
      781 taggctttaa gcccttcaaa gacctcctgc ccataatgaa gaacgagggc cgcggggtcc
      841 atgggaatgg ggcgataggg ctcgatgcgg ggattcttcc atccttcgcc cttcctgtaa
      901 tccatcataa aaagataatc cgtgaagatc ttgccgaaac ccagctgggc gtccgggtcc
      961 ggtttctttt tcagcttttt ggggtctgca tgggtaatca aaatttcggt catagaaggt
     1021 tatcctttct catacacttt tggtctgagc caattttttc agccgatcgg cctcttttcc
     1081 ccctttcctt ctcacgccga aatgaaaaaa agcgaggtga cacggcagga gattcaatac
     1141 ctatatcata atcaaccccg gaaaaaaagc ctcaataacc ccagcccacc cgggccagag
     1201 tgagaacatg gacctcggta gcccctgctt cccgcagaac gtaagcgcac tcctggaccg
     1261 tagccccggt ggtaaagaca tcgtcaatga ggaggattcg ttttcccttc acggcttccc
     1321 tttttttgac ttcgaaggcc ccgcgcacgt tcttccgccg ttcggctccc gataactgag
     1381 tttggggttg agtctggcgg gcccggtgga gagcggtaaa gttgagagga atcgaccgac
     1441 gccggctcac ttggcgggcc aggagcaggg attggttaaa ccctctctcc cgcaggcggc
     1501 gggtgtggag ggggacgggg atgacccaat cgttttcggc gagttcaaaa tcggggtcct
     1561 ggtactcagc caggaggttt cccaggggct tcgccaggcg agtcaaaccc ccgtatttga
     1621 gcctggaaat ggcttccatg atcacccctt cataaccgca gaccgccctg gctcggctga
     1681 aatgtctttc ttcgctcaga caccccgtgc agagatgatc gacctcggaa gacgacggaa
     1741 acccgatgcc gcaacgaagg cagcgagggg aagagaaaaa ttttacctgg gagagacaat
     1801 ctgggcaggg gcgatcgggg gtagaagaat ccggcgaaag gccgcagaga ggacacttgg
     1861 ctggaaaaaa gaaatccaaa accgctcgcc agaaaggggc ggggctcata tccttgccgg
     1921 aaaatgatag aaagggatga ccggcatctt ctgggagaat tcgggaaggt gcatggagta
     1981 acttccccag gaatggcagg cttcgcagaa acccgaccac tcggcttggg tcctcccgca
     2041 ggacgaacac tggtaagccg gcagggggaa ttttttcgta tccagggctt tctcgtagga
     2101 ttccatggat tggacaaatt cccctcgccg gtgataggcc tgtcccagga ggacgaaaag
     2161 ctcggagaag aggacccctg cggtctccag gtcccggagc tgctcgatgg cttcatccac
     2221 catttccagg cgaatgagca gccggctgaa gaagaatcgc aaaacactat cttccgggcg
     2281 ctgacgcata taatcccggt acaggtccag ggccttctgg gggttggcca tggaaaggta
     2341 gagagattcc agcctctcca ggaagaccgg gttccaggaa gcctctaagg cttttttcca
     2401 ggtctggctg gcctcttcga aggagccctg tctctgaagg acatctccca gggaaacatg
     2461 ggcggggaag aaagtcgagt cctccttgag aacctcccgc agggcctttt cggcatcctt
     2521 gaattctccc ttctgggcca attcctcggc gaatttatac tccattccag aaaggaacct
     2581 cttttcggcc agggcgtatt ctttttccct ggtgtgtttc atcacgctct tctgggtatg
     2641 gtaagcctca tcccagttcc cggtaatgat atggatatct ctttttttgc ggagggcctc
     2701 ccggttggag gggtcgcggt ccaggatggt atcaaggacc tccaaggcta cggcgtatct
     2761 tctgaggcct acattgttct tgaccaagtc taagagaagt tccaggctct ccggttcgat
     2821 ggctttggcc ttggtgaggg tgtcgatggc ctcggaatac ttaccctcta gggaatagag
     2881 gttggccagg gagatatgag cgggcaggtg gttcggccgc gtgtcgataa tcttggagag
     2941 cgtctctttg gctcggggga tattcccttt cagcatttcc tccagggcat cgctgtatcg
     3001 ctcccggacc ttgccgtcct tgcggtcggt tttggaggag cgccacagct gaaaggctct
     3061 ccgggcatcc acaaacaggg ttaccaggaa gacaaagaag gcgccaatga tgaaagctcc
     3121 aaaggcgatc agcgccgggg agatgatcag gtgcaggtcc tgccaaaggg taaactccac
     3181 ttcgccggga ttgaggtaaa agaggtagcc aaaagctatg aagaccaaaa ggaagacgat
     3241 gaagtgggtt cgggaaaaca tgattggctc ctacgattta tggtaaggct ctccccgcag
     3301 aagggaaaaa gccctgtaga cctgttccaa aaggagcatc cgggccattc catgggccaa
     3361 agtcatggtt gagaaggaaa ggcggaaatc cactttctcc ttcaaagatt cctccaatcc
     3421 gtagggtccg ccgaggatga aaacgatttc cctggaaccc cactgacccc acagctccag
     3481 attccgagaa aaggcctccg aggtaagagg ctttcccttc tcatccagag ccacggtgaa
     3541 agcttcgggg ggaaggtttt cccggatcct ccggccctcc tgccggagaa tgtattcttt
     3601 tctccccttc ggagcgatcc tttcttccgg gatggggacc agcctgagct ccgcatagcg
     3661 ccggatcctc tcctcatata ttcggcatcc ctctcggata taagagtcgg atgcccgacc
     3721 cacgaagagg aggaccaggc gaaatttttt catcgcgaac gtttcggccg gacctttttg
     3781 gacaatggaa gctcaacccg ggtcgcttcc gaccaaagcc tttccagatc gtaaaactcg
     3841 cggacgggct ggaaaaaaat gtggaccacc acatccccat agtccaggag ggtccagcgc
     3901 ccttctcgct tcccctcgat tcccagagga tgaatccctt ctttgccaag cttctcttcg
     3961 atatggctgg caatcgcctg gacctggcgg tctgagttcc cgctacaaat caggaagtaa
     4021 tccgtaaagg aagaaaggtc tttgacctct agaatgacca agtcgatggc cttgcggtcc
     4081 aaaagggctc gggcacagag gaaagctttc tcttgggagc tggagggaat caacctgccg
     4141 tttcgacgtc cttgttctga tagagtttat tcgaaagaat gaatgcctct acagccggcg
     4201 gaaccagata tttgatcgat cttccttccc gaatccactg ccggatccga gaggaagaaa
     4261 tgtccattcc cgtgacctcc gcgggataca cacagaaccc ggatttatgg gcgtatccgc
     4321 ctttttcggt atcatagcaa aattcgtttg ttagttcaac gggaagatgc tcccgggaaa
     4381 aaattttttc gaaacccggt cgggtcataa ctacgaaatg acagaggcgg aaaagggccc
     4441 catactcctt ccaggtgctc acctccagga aggcatccag acccacgatg aaatagacct
     4501 gggtgccggg accggattgg tggcgaaaat gctcgatggt ctgaatggaa taagacttcc
     4561 ccggacgttc caattccacc tcggaaaagg cgaagcgggg attatccgca atggcccgtc
     4621 ggaccatttc ggcccgagag gtggcgtcga ggattccttc cttttttttg tggggagggt
     4681 aggaggaagg gataaaaaag acccggtgga gatcgaagga atgactgacc tcttcggccg
     4741 cccgaaggtg gcccagatga atggggttga aggttccgcc gaagagcccg acctttttca
     4801 tgtgcgcact tgtcccgagc cgtagacgat gaatttggaa gtggtcaggt cctccacccc
     4861 catgggccca aaggcgtgca gcttggtggt gctgatgccg atttcagccc ccagccccaa
     4921 ctggaacccg tcgctgaacc gggtggaagc attcaccaag acggtggagg agggaacctc
     4981 ccggaggaac ctctgggcat tggtataatt ctgagtgacg atggcctcgg tgtgggagga
     5041 accgtagcgg atgatgtggc ggatggcttg gtccaagtcg tccacgattt tcaccgcgag
     5101 aatcaaatcc agatactctg ccagccagtc ttcttccgag gcttccccga tgccgcccag
     5161 gattcggcgg gtttccgggc atccccgaag ctccacgccg gcggctttaa acctctccgc
     5221 cacgggagga aggaagaccg gcgcgatctg gcgatgaacc agaagggttt ccatggcatt
     5281 gcacaccccg ggccgctgaa ccttggagtt aaaagagatg ttctgggcca tgtccaggtc
     5341 cgcggaggcg tccacgaaga catgacagac tcctttataa tgcttgatta ccgggattct
     5401 ggatttggag accacgaagc ggatcaattc ctctccccct cgggggatga tgacgtcgat
     5461 gtagtcttcc agttgaagca tctcctccac tgccaggcgg gatgtgctgg gaacgacctg
     5521 aatggcctca gccgggattc cctgttcgcg gcagaccttt cggaggactt cggcaatggc
     5581 caggttggag tggatggcct cagacccccc ccgcaggatc accgcgtttc ccgctttcag
     5641 gcatagcccg ccggcatcgg aggtcacgtc gggacgggat tcatagatga tcccgattac
     5701 ccccaagggg atgcgcattt tccccacggt aagaccgttg ggacgggtcc acatccgatt
     5761 cacttctccg acgggatcgg gaaggagggc gatttctttc aggctggcgg ccattctcat
     5821 gaggactgca tcagaaagag tcaggcggtc gatcatggcg gaggagagcc cttctccttt
     5881 ggccgctttc aaatccagaa cattggcttg cttcagccca ggggccgaga aaagcaaggc
     5941 ctcggccatc ccctccaggg cagagttttt gaccgaggtg gacagattcc ccaaagcata
     6001 gctggcctcc cgggcccgct gtccgatcat taccatttca tccctcagag acatgccggc
     6061 ctcctcatct caaaaagaag cttctcccga atgaagcacc aaatttccag cattcaaacc
     6121 aaaataacca gcaataccca ccaaatacca aacttttctc gaaggcctcc atttttcaaa
     6181 ttccctgttt cgctcactgc gttttggggt ttttgaattt ctttgggatt tggcattcga
     6241 gattcggagt ttgatgatat cccggtgaca cacagaccag atcgtcccga tgaacgattt
     6301 cgtcgctgta tttataaccc agccgactct ccacctcgga agttttcagc cccttgatct
     6361 tgtttatttc cgaggagtca taattggtca gccctttggc aaactcctgc ccgttcacgt
     6421 cggagagggc gaccgggtct cccaggctga actttccctc cgcggacaga accccggatg
     6481 ggaggaggct tctccccttt tgcaggatgg ccctcttggc tccctcatcc acgatgatcc
     6541 tgccctgggg ctccagagtg aaggcgatcc agtgcttccg gctggtcagc ttattgacct
     6601 gggaggtgaa gagcgttccc aattcttccc cccgaaggat tttttccagc gccccttcca
     6661 tcataccgtt ggccagtacg gtcgggaccc cgaagagagc cgccttgcgg gcggcttgca
     6721 gcttagtgac catcccgccg atgctgaagg ggctcagggt tcccgtggcc ttctgttcca
     6781 tctcctccgt gacctcatgg acggagcgaa tgagtcgggc gcttttatgg accctcgggt
     6841 ccttgtcgta aaggccgtca atgtcgctca gaatcaccag gaggtcggcg ttgaccaggt
     6901 tcgtgaccaa ggccgataga ttatcgttgt ccccgaactt gatctcttga acaaccaccg
     6961 tgtcattttc gttgatgatc gggatcaccc cgaaatccaa aagggagaga agggtgttgc
     7021 gggcgttgag atagcggttg cggttggaga gatcatcccg ggtcagcagg acctgggcaa
     7081 ccttttcgcc gtagaaggag aaggcctttt catagctcca catcagggca ctctggccga
     7141 tggccgcaga ggcctgtttt tcgggcatca tctttggttt ttcaagaagg ccgaggcggc
     7201 ccatgccggc ggcaatggct cccgaagaaa ccaaaaccac ttcgtagccc cttttcttgg
     7261 ccgctgaaat ttcctgggcc aaacggtcga agaccggctg gctcaaggtt cttccctttc
     7321 cggtcagaac ccggctcccg accttgagaa cgacccggcg gactttttct aagatgctct
     7381 tccggttcaa ttggaccttt ccttcttcaa acctttgtcc aagtgtcggg ttagggcgcc
     7441 gagaagtttc ggcaagcctt ctcccgatag gcccgaaatg gggtaggtcg gctttttgat
     7501 tctcttaaaa aaagccatga ctgccggcag tctctttttg ctttccggat cgtcgatctt
     7561 attgagagcg acaacctgcg gtttacgggc cagccccggg tcaaagagcg ccagttcccg
     7621 gttcaaaatc cggtaatcct cgaggggatc acggggattg agggaagaga ggtccaggac
     7681 gtgaagaagc agagaagttc gttgcacatg ccgaagaaat ttcagcccca gacccgcccc
     7741 ccggtgggct ccctcgatga tcccggggat gtcggcaatg acgaagggga ggtggtccct
     7801 ccaggaaacg acgcccagat ttggaaccag ggtggtgaaa ggataatcgg cgattttggg
     7861 ccgtaccgag gagaccttgg aaatcagagt ggatttgccc gcgttgggat atccgatgat
     7921 ccccacatcg gccatgagtt tcaattccag cagcacccag caggactccc cgggctggcc
     7981 gggttcggca tgacggggcg cctgatgggt ggaagaggcg aaacgggcat tcccccgccc
     8041 tccctttccc ccccgggcta cgacgaaggt ctcgttttcc ccggtgaggt ccctgagctc
     8101 ttttccactc tgcccgtcgc ggacgattgt gccgcgggga acgggaagaa ggagggcagg
     8161 ggcgctgcgc ccctcctgat tttttcctct tccgtgctgc cctcttttgg cgtggtaatg
     8221 ctggcggtaa cgaaaatcga ggagggtgga gagttggtcg tcggccttca gaatcacatc
     8281 cccgcctttt cccccatcgc cgccatcggg acctccgcgg ggaacgtatt tttcacggcg
     8341 aaagctgatg cagccgtttc ctccatcgcc ggcctggacg aaaattttgg cttcatcgac
     8401 gaacttgatc ggtcagaccc ccggtcttcg cgggcccccg cggagccccg ttgacaagcg
     8461 gaattaagcc ggggcccctt cggcctggga aggataaacg ctgaccttct tcctttcccg
     8521 atccagtctt tcgaaggtga caattccgtc gatcttggca aacagggtaa aatccttccc
     8581 tacccccacg ttctttcctg gatggatttg agttcctttc tgtcggacca gaatggaccc
     8641 tgcatggacc ctctgcccgc caaatcgctt cactcccagc ctttgtccgg cgctgtcccg
     8701 gccgttgcgc gagcttccct gtcctttctt gtgtgccata tgaaccccct taaagacgaa
     8761 tgcctcaagt gcctcaagtg cctcaagtaa agacgactct ttttgaactt gaggcactta
     8821 aggcatttga gcgcacttta ctgcaaattg atttccttaa tcctcaaact agtaaaggat
     8881 tggcgatggc ccgtcttttt atcgtatccc ttccgccgtt tcttcttgaa gatgatgacc
     8941 tttttggccc ggtgctgtcc aagtatttcc cccacgactt ttacgttgga gagcatgggt
     9001 tgaccggtaa ggacgttttc tccttcggcg tagaaaagga cgtccttcat ctccaccgac
     9061 tctcctttct ggccggcaat cttttcgatc ttaatgatgt cccccggaga gatcttaaac
     9121 tgtttcccgc ccgtgcgaat aatcgcgtac acgttacccc ctgaaattcc ctcgagaata
     9181 cccctcctcc caacccaaaa gaagagaggc aaagggaaaa tattcaaact attatgttag
     9241 gatttcagac ttgggtttgt caacggcaaa atgaccgttg tgactttgca cctcatactg
     9301 ctccaggtga aagtcatttt tcccggtgat ggcgatgcgc tttttgtagc gtttctccag
     9361 cttctccagg tctgcccggg cctcatcgta taaatggttg gccacgttgg gatggacgtg
     9421 gaccaggatg gtctcttcct tcaggtctcg aatatctcgc tggatttcgc gcaaaatctc
     9481 ctgacagatg gtggaacgcg atttgagaaa accccggccc tcgcaataaa agcacctctc
     9541 gctcagaatc cggcccaaac tttctcgggt tcgtttgcgg gtcatctcca ccaggcccag
     9601 ttcagagatt ttcaagatat tggtcttgct cttgtccttt ttcagagctt cggtgagcgc
     9661 ctgaaagacc ttttctcggc tggattcttt ctccatgtcg ataaagtcga tgatgatgat
     9721 ccctccgatg ttgcgcaacc ggagctgata ggcgatctct ttgaccgcct ccaggttggt
     9781 tttcaggatg gtgtcctcaa gattcctttt tcccacatac cggcccgtgt ttacatcgat
     9841 gacggttagg gcctcggtca ggtcgatgac gatatacccc ccggatttga gccagatctt
     9901 gcggcctaag gcgcggctga tttccatctc aattccgaag taatcgaaga gaggctcttc
     9961 cttttcgaag atttcaatgg aggctttcag ctgcggcatg aaggtgtcac agaattccat
    10021 gatcttctgg tactcctccc gggagtccac gaccacccga tccacctcct gggtgaagag
    10081 gtcgcggatg gccctcaggc tgatatccag atccttgtgg atgaggctgg ggacggggct
    10141 caccgccttg cgcttctgga tgttggccca cattttcaag aggaaatcca ggtcctggcg
    10201 cagctcggcc tcacctttcc cttctgaggc ggtgcgcacg atcagcccgc atccgggcgg
    10261 cctgatctgc tggatgacgt cccggagccg ctttctttcg ccctcgtttt caatccgccg
    10321 ggataccccg atgtgatcca ccatgggcat gagaactaag tggcggccgg gtagggagat
    10381 gtgggacgtg atccgggcgc ccttggtgcc cagcggttcc ttggatacct gaaccaggac
    10441 ttcctgccct tcgtgcaata ggtcttcgat atggaaagga gtgtcaagat tcaggacttc
    10501 ttcctcatcg gccggggagt gcccgtttcc gtcttcctcc cgcccccgca tgaggatctc
    10561 caggtcatcg aagtcctggt gtacgtcgga aacatagagg aaagcagctt tttccagccc
    10621 aatgtccacg aaggctgcct gcatcccggg aaggactcgt accactcggc ccttgtagat
    10681 gtttccgcca atcccctgtt cggagcttct ttccacgtaa agctccacca gcacatcgcc
    10741 ctccaggagg gcgacccggg tttctccgga actggcatta atcaggatct cgttggacat
    10801 ggcctgaatt cttaaaacca acctgaattt ttttgatgga aaacccggat ttcccctcct
    10861 ccgcccatcc cagaaggtaa cccagagctt cctccggtct taaggtacct tgggtagatg
    10921 aaagcatccg gatttggacg gtcctttcgt caactacagt taccgattcg acgaattttt
    10981 ttaaatcaat gtctttcttt tttctcgacc aagtaatcca aaaggaatcc gctttcccaa
    11041 atccggcgat tttttccgta gggaccccga ccgggtcggg gaaaacaata ttatatagaa
    11101 tataagccat attatcaaaa attgatggaa aagacaaggg aatttcctcg atttccaaaa
    11161 attccacccc gtgggggagg acttcattca ggcgctgaag ggcccttcca ggctgaaatt
    11221 tttcccaaac atggaagtcc agatactccg cccaactctc gtatcccacg gggaggggcg
    11281 gtccaaaagc gatcttgggc aagggatgga aaccctggga gaagaccagg gggatcccgg
    11341 cccgccgaaa ggcccggatg atcccctggg aaaaatccag gtgtccgagg aaccgaatcg
    11401 gtcccaactt tgtcagccgg atgcgaaacc gtatgaccgg aagagaggat tcggtccgcc
    11461 tggtggccgc gggtgccagg ttaaggaccg tttcccattc tgcaaaccgg ttggaaaggc
    11521 catccactcc ataacagact ccgcaaccgt tgcaggaagc ttggcggcaa tccggggtac
    11581 aaaggtttcc cagggccttt tctctttcct gcttaaggaa ggatttgctc accctggagt
    11641 gcaggtggtc ccagggaagg atttcatcca catccctttt ccggaggtaa aaagaggggt
    11701 tcacaccgct ctggcgaaga gcctcctccc aaaggtcgta gcgaaaatgt tctccccaac
    11761 cgtcgagacg gcagcccaga aaataggccg tctccagaag atgacttaac ctccggtctc
    11821 cccgagcgaa gaccccttcc agaagactca ggcgggagtc cgaccatttg aactgcaggc
    11881 cgtggcggtc cagactcttg cgcaagaacc tctgttttct ctccacctcc tccggagaag
    11941 attgaggttc ccattggaag ggagtgtggg ctttagggat gaaggtggaa acgctgacat
    12001 tgatctgggc cgaccccttt gcccggcggg ccagcttcag agcctcgtgg cagagataag
    12061 cgattccctg taagtctttt tcttcttcgg taggaaggcc gatcatgaaa tagagtttga
    12121 ccagtcgcca gccagcggag aatacctggt gcagggtgga gcgaaggtcc tcttccgtgt
    12181 tccctttgtt aatcacccgg cggaggcgat cggtacccgc ttctggggcc agggtgaagc
    12241 cggtctttcg cacctcccgg acctgctgga tcagagtggg ggtgagggtt tcaacccgca
    12301 gcgacggcag ggagacagcg attcttccct cccgggtatg ctccagaagg gaagagagaa
    12361 gcggttcgat gcaggagtaa tctccggtgc tgagtgagag aagggagact tcatcgtacc
    12421 ccgtgctctg gaggccacat ttcgccaact ccagaatttt tcgcggggat cgctcacgca
    12481 gggggcgata aatcattccc gcttggcaaa atcggcatcc ccgggtgcag ccccgggcga
    12541 tctctaagtt cagacggtca tggattactt ccatgagagg aaggatgggc cgggaaggga
    12601 aggggccctt atcgaggtcg gcgagaaccc ggcgagtcac atgagaatac ccagattgaa
    12661 tgggaaggat ttgccggagc cgcccgtcat cctcatattc caggttgaaa aaggagggaa
    12721 tatagacccc ttcaatacct gccagcctct ccagaagggt ccttttactt tccccctttt
    12781 ccttggaggc gacaatcgcc tggcagagct ccagcaccac ctcttctccg tcccccagga
    12841 cgaaaaggtc gaagaaatcg gccaggggct ccgggttcaa ggcgcaagga ccgcccccta
    12901 tgaccagggg gtcattttca tctctttgtt gggcccgcag ggggatcccg gccaggtcca
    12961 ggatgtttag aactccagtg tagttaagct cgtactgcag agaaaagccc aggagggcaa
    13021 attctttcag gggacgggaa gtctccagcg aacaaagggg aagccctctt tcgcggagga
    13081 gattttcaaa atccggccag ggcgcaaaga cccgttcgca ggcgatttct gggttggcgt
    13141 tcaagagaga ataaagaata tggaatccga tgtgcgacat ccctacttca taaacatcgg
    13201 ggaaggccaa ggcgaaggtc aactttactt ttcgggggtc cttaataacc gaatgggtct
    13261 ctcccccgag ataccgggag ggcttagcaa ttctcagaag gtcttcggct ttcaccgaaa
    13321 aatattaaca ttcggctgca tgcggggcaa atgatttatc ttagtttgaa gctcttttag
    13381 agtccgtaga cgggaatgac tggagggtgc aaacaccgga accttctttc tcttttccaa
    13441 caggcggatt atgcccattc gcggatttat gagctggcca tgggcatccg cctaatgagg
    13501 atcctgagga ttcggcaggg atcgagggaa ccttgccaac aggaagggcg gcattggagg
    13561 aagctcagaa cactaagcgc atggcctcat tctctttttt tggccagacg caccgcgctt
    13621 ttctgttgct gctcccttag gggctatgtt agaaagaaag atcattcttt gggaaggaag
    13681 gagaccggca tgaacctaaa agcgaaggtt tatgtggaaa accagaaaaa gagggtcctg
    13741 gaggccctca acgcccgcct tgcgcgtctg aaagaaaaag ggttgaagga aaacatcgtt
    13801 cagaaggatg caacagttcg aaaactgaaa gctcagctcc ggaaggcgga ccaccgcttg
    13861 gccagcatcg cggcccagga aaaactcaac caggagaggg tccaggccaa attggacagg
    13921 caggctgcgg aaaaagcggc caaagaagcg ccccggcccg aggcggaaaa gggggagccg
    13981 gagaaaaaag aaaagaaggc caagaaagag aaacaggaaa aaccggccaa gccggaggga
    14041 aaggtagaag gagagaagga gaagaaagaa aaacaggaaa aaccgaaaaa agcgccggac
    14101 aaggaagaag agcaatccga aggttctccg tagcttaagg ccttccgccc aaggattttc
    14161 gagaattcga ccgccgaacc ggggaggcga ataaaaagct ggaggtgaat atgtctgttc
    14221 tggttacggg agggtgcggt ctcatcggct cccggttgac ccgtatgctg gtggaaaaag
    14281 gagagaaagt tcatgtcctg gacatccacc ttcgccccca atgtttttcc ggaatcagtg
    14341 accggataga attcgttcgc gcggatgtgg gcaattttgc caaggttctg gaagcggtga
    14401 aagagtgctc gcccacggtg atcttccacc tggggggcat gctctccctc ccctccaacg
    14461 ccgatccaca gtcctccttt tccaccaatg tggccggcac ctatcatgtc ctggaagcgg
    14521 cccgtctgtt cagagttccc aaagtcatct tcaccagcac ggttgcgacc tacgggctgg
    14581 acatccagag caaaacgatt aacgattcca cccttcaaag gcccattacc atgtacggct
    14641 cgaccaaagt tttcgccgag cttctggggc ggttctaccg gaacaagtac ggagtggatt
    14701 tccgagcggt ccggtatcca tcggtagtcg gtcctggggc caagaccgcg catatttcca
    14761 tttataacgc ttgggcggtg gagaaagcct tcttcggaga accctacgag gtctttgtgg
    14821 cgcctcatgt ccgctgccct atcatttact tcaaggatgc agcccggtct ctccttctcc
    14881 tggaagcagc tccccccgag gccataagga ccgtctgcta ccttctcgcc gggattcagc
    14941 ccatgatctc cgccgaggaa ttggtggggg agatcaaaaa gcattttccc caggccctgc
    15001 tcaccttcaa gccagaccct ctggccatgg aggtgcatgg caaatcacaa ggggttgcct
    15061 atgaggactt caatgcgcag aaagaatggg gctggaagac agagtatacc cttcccagga
    15121 tgatcggcga cttctatgct gaattgaaag ctcaccccga atggtaccgt tgacccctcg
    15181 gtccaggggg agccaccatc agggtctgcc gaggggagag gagattcggt tttccgcaaa
    15241 gaaggcaata tttccgaggc aaaaaaaagg ggatgacccc cgctgagggt catcccccct
    15301 tttagcgttt gaggattcta tttcttcttg gcccccaaaa tcccttttag ggcggcagcc
    15361 gggacggctt tcaccacttt cttagccgga attttcagag gctctccggt ctggggattc
    15421 cggccgattc gggctttccg gttttgaact ttcacggtca caaagccggg cagcgccagt
    15481 ttatcccctt ttttgatgat ctttacagct aagccggcaa gagcatcgag aaccgcagct
    15541 ccctgcttct tggtgattcc cacctcttcg gccaaatagg ctacgacctg cgacttggtc
    15601 atgggcatag gcttttcctc ctttcgtaat cgatgaaatg cctttcctct cgccctcttc
    15661 ccgactcatc cccgcttcct cgtggcgaat ctccccttta tcactttgcc gggacgatgt
    15721 cgacccgacg gttttttgcc caggcctcct cattgtgccc tgggtccagg gggcgttctt
    15781 ccccgtagct gatcgtggcc attctctggt tggagattcc cagcccttcc aggaatgatt
    15841 ttaccccttc tgcccttttt tgtcctaacg ccaggttgta ctctgccgtt ccccgctcgt
    15901 cgcaatgccc ctccagcctc accttcacat tggggttgga cttgagccac tggctattct
    15961 tggccatgac ctctttctgg tcttcgcgga tggaccactg gtcgaaatca aaaaaaaccg
    16021 gttccaggct gaccgttttc gaggcggctt cccgagcggc cgcttcccgc ctggcggcct
    16081 cttcctgcag agccttttcc cgcaaggcct tctccctcag ggattcctcg cggaggctct
    16141 cttccgcaag ctttttctcc ttctccagag cggcagtctt atccggagca gcagcctccg
    16201 gcctggtctc tgctggccca ggctcaactc gggagggagt atagacgggc gaatcggaac
    16261 ggattgtttt tttggcgcat cctatgccgg gcccgagggc caggatcagg agtcctatca
    16321 gaagaagtct gatgcctttc attttctttc tcctgttgca ggtatttaat aaaagcgtat
    16381 cgactgaaaa tcggagtgtc aaggataaaa tcaatcgaga atcccccctt ggcagaaatc
    16441 ggagagtttg ttaaggtgaa atcaactgga cccatcaaga gggagggaag catgaaggtc
    16501 ttgggaatcc atggaagtcc gcgcaagggg ggcaacaccg aaatcctgct caaagaattc
    16561 ctccgaggct gtaaagaggg aggcgccgag gtggaagaaa ttttccttcg ggagctgaaa
    16621 atcagcccct gcctggaaat ctacgcctgc aaaaaagatg ggcaatgccc aatccaagac
    16681 gacatgaagg ccctctaccc aaaattggcc gaagccgatg tactggcggt ggcgtccccg
    16741 atcttcttct acgcggtcag cgcccatttg aaagccatga ttgaccgttg ccaggcttta
    16801 tgggccaaga aatatctgct caaccaaccc attgcccccg gaaggtccgg gaggaaaggg
    16861 gttttcctgg cggtgggagg gtccagaggg ggtaagacct ttgacggggc gctcctgact
    16921 atgaaatatt tcttcgatgc cttggatatg accttccacc gttccctcct ttacagagag
    16981 gtagatgcca aaggcgagat cctgcagcac cccacggcaa tggcggaagc gtacgcattg
    17041 ggaaaagaat cagttaggaa aattgcagaa ttcgaaatga aaagtgcaaa atgaacccag
    17101 atgcacaatg atctaatgac caattgtcat tctacaatgt tggatgagat ccaggcatca
    17161 gatgatgact cgcggcgaag cgcaggccaa gggcaaacgg caacggactt cggaaaaagg
    17221 acccattcat gaaaagaagt tcctggcagg atagtttcat accccttcaa gagggattgt
    17281 ccctgaatga ttttcaagga ggcagcggga atctagtggt tatctcccct catcccgatg
    17341 acgatgttct g
//
LOCUS       DASPRR010000265         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_65010, whole genome shotgun sequence.
ACCESSION   DASPRR010000265 DASPRR010000000
VERSION     DASPRR010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65010"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VEL68_10355"
     CDS             complement(<1..143)
                     /locus_tag="VEL68_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HXZ36410.1"
                     /translation="MKKKVIIDCDVGVDDALASILAFHSPELEVMGVTGVHGNVSLDK
                     VMK"
     gene            complement(215..886)
                     /gene="deoC"
                     /locus_tag="VEL68_10360"
     CDS             complement(215..886)
                     /gene="deoC"
                     /locus_tag="VEL68_10360"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HXZ36411.1"
                     /translation="MPTTKEELARMIDHSLLRPQSTRVELKKLCGEALQYGFKAVCVN
                     PVHVAGAFSILKGSEVIVCSVIGFPFGTHPPQIKAAETREVIRLGAQEIDMVIQVGGL
                     KEGRNSEVVEDIRAVVEAASGYTVKVILETCYLTEEEKIRGCKLAMEAGASFVKTSTG
                     FGSAGATVEDVRLMRKVIGKDFGLKAAGGIRTLEDALKMIEAGANRLGLSGSVAIIRE
                     MEKGN"
     gene            complement(946..1446)
                     /locus_tag="VEL68_10365"
     CDS             complement(946..1446)
                     /locus_tag="VEL68_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36412.1"
                     /translation="MKGRLLFPILFFSLVAPGPWLPPVWGQEEHHGADSSFQLRDLVI
                     LWGILKGPDEDRSRVYIKIIRTGSEPGPWTSYRLEAVDPFSQEKEWVTPRENLGKENA
                     VKEIRSSFREKTGRRLYFYAGQGKEEKPAAIIFYQGVPDTTPEFLTEKEMEDYFDQAL
                     KRMMKQ"
     gene            complement(1443..2543)
                     /locus_tag="VEL68_10370"
     CDS             complement(1443..2543)
                     /locus_tag="VEL68_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36413.1"
                     /translation="MEAVSIRLDRISKKFSHRVKGEVSAVSDVSLQVQPGEFLTLLGP
                     SGCGKTTTLRMIAGFEHPDIGRVHIGEEDVTDKMANQRNIGFVFQNYALFPHLSVFEN
                     VAYGLRVKGMGEAEIERSVIEVLALVGLKGYERQFPHQLSGGEQQRVALARAVIIQPR
                     VLLFDEPLSNLDAKLRVHMRSEIRRLQKALSITAIYVTHDQEEAMAISDHIAVMNEGK
                     IIQIGTAEELYLQPNSPFVARFIGKINPLPAEVKGTGPGQVTIEVFRHLFSVPGEGDR
                     FQSGQKVWTFIRPESVELSPDLQVGDFQGVVRERTFLGEKVDYLLEVDGHELNATSYD
                     PLRHGTFSVSQKVGVRLDPRLLKILEDEGCRP"
     gene            complement(2545..>2797)
                     /locus_tag="VEL68_10375"
     CDS             complement(2545..>2797)
                     /locus_tag="VEL68_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36414.1"
                     /translation="AGLGVVFIRSMTAISATIFLISINWTLITVRILEKMTELELGVA
                     AAFSILVVVIVFAVIGAISLFLRIFRQPGAPDMANILGG"
BASE COUNT          611 a          843 c          721 g          622 t
ORIGIN      
        1 tttttcatta ccttgtccag ggaaacattc ccatggaccc cggtgacccc catcacctcc
       61 agctcgggtg aatgaaaagc aaggatcgat gccagggcat catccacccc cacatcacag
      121 tcaatgataa ctttcttctt cattcagagg atctttccga aaataaggga ggcaggaaac
      181 cagcaataac gagaaccaat aaccagatcc tttatcagtt ccccttttcc atctcccgaa
      241 taatcgccac gctgccgctc agtccaaggc gattggctcc ggcctcgatc attttcaagg
      301 cgtcttccag ggtccggatt ccgccggccg ccttcaaccc gaaatccttc ccgatcacct
      361 tccgcataag cctcacatct tccaccgtcg ccccggcgga gccgaagcca gtggaggtct
      421 tgacgaaaga agcgccggct tccatggcga gcttgcagcc ccggattttt tcctcctctg
      481 tcaggtagca agtttcgagg atgactttca ccgtgtagcc ggaagccgcc tcgacgacag
      541 cacggatgtc ttccaccact tccgaatttc tcccctcttt caggccaccg acctggatga
      601 ccatgtcgat ctcctgcgct cccaggcgaa tgacctctcg ggtctctgca gccttgatct
      661 gcgggggatg agttccaaag gggaacccga tgacggagca gacgatgacc tcgctaccct
      721 tcaggataga gaaggccccg gcgacatgga ccgggttcac acagacagcc ttgaacccgt
      781 actggagagc ttcgccgcag agtttcttca attcaacccg tgtggactgg ggacgaagga
      841 gggaatgatc gatcatccgg gctaattctt ccttagttgt gggcatcctt tttctccttt
      901 gaataagaga acccaagtga caggttctag aagcaccaaa ccttctcatt gtttcatcat
      961 tctcttcaac gcctggtcaa aataatcctc catctccttt tcggtcagaa actccggggt
     1021 ggtatccggg accccctggt agaagatgat cgccgccggc ttctcttcct tgccttgtcc
     1081 ggcgtaaaag tagaggcgtc ttccggtttt ttcccggaaa gatgaacgta tttccttaac
     1141 ggcattttct ttcccgagat tttccctggg agtcacccat tccttttcct gagaaaaggg
     1201 gtctacggcc tccaggcgat aactcgtcca cggcccgggt tcgctcccgg ttcggattat
     1261 tttgatgtac acccgggagc ggtcctcatc cggccccttt aaaattcccc acagaatgac
     1321 caggtctcgc aactgaaagg atgaatcggc gccgtggtgt tcctcctggc cccaaaccgg
     1381 gggtagccag ggccctggag cgactaggga aaagaacaag atgggaaaaa gcaagcgccc
     1441 tttcatggcc tgcatccttc atcttcaaga atctttagaa gtcgggggtc caaacgcacc
     1501 ccaactttct ggctcacgga gaaagtgccg tggcgcagag ggtcatagga ggtcgcgttc
     1561 agttcatggc cgtccacctc cagcaggtag tccacctttt cacccaggaa agtacgctcg
     1621 cgcacgaccc cctggaagtc cccaacctgt aaatcaggag ataactctac ggattccggc
     1681 cggatgaaag tccacacctt ctggccggat tgaaatctat ccccctcacc gggtacagaa
     1741 aaaaggtggc gaaaaacctc aatcgtaacc tggcccgggc cagtcccttt cacttcggcg
     1801 ggtaagggat tgattttccc gatgaacctg gcgacgaaag gagagttcgg ttgcaggtag
     1861 agttcctcgg ccgtcccgat ctggatgatc ttcccctcgt tcatcaccgc gatgtggtcg
     1921 gagatggcca tggcctcttc ctggtcgtgg gtgacataga tcgcggtgat ggacagggct
     1981 ttctgcagcc tgcggatctc gctccgcatg tggacccgga gcttggcgtc caggttggac
     2041 aagggctcat cgaagagaag tacccgaggc tgaatgatca ccgcccgtgc tagggccacc
     2101 ctctgctgct ctccccccga aagttggtgg ggaaactggc gctcgtatcc tttgagcccc
     2161 actaaggcca ggacttcaat taccgacctc tcgatctcgg cttcccccat tcctttcacc
     2221 ctcaggccgt aggccacatt ttcaaagacc gaaaggtggg gaaagagggc gtaattctgg
     2281 aatacaaacc cgatgtttct ctggttggcc atcttgtcag tcacgtcttc ttccccgatg
     2341 tggacccggc ctatgtccgg gtgttcaaaa ccggcaatca tccgcagggt cgtggtcttc
     2401 ccgcagcccg agggccccag gagggtaagg aactctcccg gctggacttg cagactcacg
     2461 tcgcttaccg cggacacttc ccccttcacc cggtgggaaa atttcttgct gatccgatcc
     2521 agacggatgc ttacggcttc cacattatcc ccccaaaatg ttggccatgt ccggagcccc
     2581 cggttgacgg aagatccgga gaaaaaggct gatggccccg atgacggcaa acacaatgac
     2641 cacgaccagg atggaaaagg cggcggccac gcccagctcc agttcggtca tcttttccag
     2701 gatgcgcacg gtgatgagcg tccagttgat ggatatgaga aagatcgtcg cgctgatggc
     2761 ggtcatggag cggatgaaga cgacccccag gccagca
//
LOCUS       DASPRR010000266         7222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_867266, whole genome shotgun sequence.
ACCESSION   DASPRR010000266 DASPRR010000000
VERSION     DASPRR010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867266"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /gene="fusA"
                     /locus_tag="VEL68_10380"
     CDS             <1..945
                     /gene="fusA"
                     /locus_tag="VEL68_10380"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXZ36415.1"
                     /translation="GDIVGAAGLKETTTGDTLCAEDHPVTLERIDFYEPVTSVAIEPK
                     SRQDQEKLDFFLQKLGEEDPTFRVKFDEDTGQTIISGMGELHLDVVVRRLLEDFNISV
                     NVGKPQVVYRETITESAEGEGKFEREIEGVMNFGHVRLRLEPRPRGAGIEFIGNSSDG
                     TIPPEFIPGIEAGVKESTGNGCVAGYPVVDLKVSLLGGTFLEGSSTPLAYQIASAMAF
                     RDGCQKAQPVLLEPIMRTEVVVPEEFVGEVIGDLGARKARIEQVQAKGKVSLIEAFVP
                     LKEMFGYSTDLRSLTQGRGTFTMQFHRFDWAPEKKKSA"
     gene            1189..2301
                     /locus_tag="VEL68_10385"
     CDS             1189..2301
                     /locus_tag="VEL68_10385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXZ36416.1"
                     /translation="MNYFWRLDCRYFIGDRPCKFKRVCEGCEQYSPMGHRILIVKLAA
                     IGDVLRTTPLLTGLKRVYPRSHITWVVDKEAYPLLKNNRCIDRLLPFDFASLLPLEAE
                     TFDLVIGLEKEPRGAAIVSKVRAKEKRGFALGPEGNPFPLNPSSEYAFFLGLSDELKF
                     HQNRKTYPELIFEIADLDYQKDDYLFFLSPEDSAFAEKFARKTGLKKSDRVIGLNTGA
                     GAVFANKAWTEEGYLGLTKQLTKEPRTKVLLLGGPGEKERNRRILKKARGAVIDTRCD
                     NTLGQFAAILNLCDLVVTGDTTAMHLAIGLKKKVIALFGPTCAQEIELYGRGEKVVSS
                     IPCAPCYRRNCEVSPNCMEAITAEEVMKKIKGLLPR"
     gene            2298..3296
                     /locus_tag="VEL68_10390"
     CDS             2298..3296
                     /locus_tag="VEL68_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXZ36417.1"
                     /translation="MTSFLRRHLKFILGLAISAGALYLALDRVDFKALGDSFQSANYF
                     FLVPAGLVQIAVFFLKAAGWRYLLMPAKKEVRLKSTVTVLIIGLMVNNLFPAKMGELA
                     RAYLIGEKERLPKTLCFSTIMVEHLLDVLVLMIFLLVLMPLVALPPWLRTSGTLVGFA
                     ALTLILVLFLVVRREEKFLGWISRILVRVPHRFRVKIQEMLQNVLQGMRVVTGRYIVY
                     AFGCLLVMWTTVFVVAYLLMAAFGLFLPFTAPIMVIIFTAFGKIIPSSPGGIGTLHYL
                     ILVVLMSFGISKEVALGCAILMHGFSFLFEVGAGFVALVFSGLSLGGVTRRAEEQA"
     gene            3293..4030
                     /locus_tag="VEL68_10395"
     CDS             3293..4030
                     /locus_tag="VEL68_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HXZ36418.1"
                     /translation="MKAMAMIPTYNEAPNIEPLLREILHQGLDIGAVVVDDNSPDGTW
                     AIVERLRKEDPRVHLIRRMNERGRGTAGVAGFKYAVAQGVDFVIEMDADFSHQPSYIP
                     FFLSQMEDCDLLIGSRLVEGGGEKGRHFLRQWITRSANAYIRLILGLSVRDCTSGYRV
                     FRRQVLEAIELDQLMAVGPPIVEEVLYKAYKRGFRIKEVPYILEERKAGESTFNRKIM
                     LNALLMMPKIRWAYRKYPLKDSSMSYK"
     gene            4430..4543
                     /locus_tag="VEL68_10400"
                     /pseudo
     CDS             4430..4543
                     /locus_tag="VEL68_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            5352..6371
                     /locus_tag="VEL68_10405"
     CDS             5352..6371
                     /locus_tag="VEL68_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019062107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36419.1"
                     /translation="MPNAQQRFRQVNATRYRLGQQAGHYESFFQRANHPSRPLAFWIR
                     YTIFSPQGRPEDAVGELWAIYFDGETQHHVAVKTEVPLSECTFKTDDFLVLVGNARLG
                     PGHVAGSVTSGAHAVSWTLDFHGDAEPLFLLPIEMYDSPLLPAKGLVGLPLAVYNGAL
                     LVDGREITVTDWVGSQNHNWGLKHTDHYAWGQVAGFDAQPETFLEVATARRRIDTDWS
                     PFMTPVVLRHRGREISLNTVAQMVRAEAAFSYSMWTFKSETDEILLEGTFAAPREAFV
                     GLGYRNPPGGTKECLNTKIASCELRVTHRQPGGATRTETFSARNRAAFEILTDEHDHG
                     VEIRA"
     gene            6501..>7222
                     /locus_tag="VEL68_10410"
     CDS             6501..>7222
                     /locus_tag="VEL68_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXZ36420.1"
                     /translation="MIRGIEGREIFHDDEDRENFISRVETLVKTTGDRILAWSLMKNH
                     VHFLLFSGPLGLPTFMRRLLTGYAVWFNRRHRRVGHLFQNRYKSIVCEEEPYLLELVR
                     YIHLNPLRSRAVKTLSELDKYCWSGHRVLVGKGRNDWQEKAYILSQFGKRERGAIRAY
                     RKFMEEGINLGRRPELVGGGLIRSRGGWSRVLSQRAKGEKEEYDSRILGSGDFVLDII
                     RDADQKMRRQIRGRGENTSIEE"
BASE COUNT         1665 a         1917 c         2033 g         1607 t
ORIGIN      
        1 ggggatatcg tcggggccgc cggtctgaag gagaccacca ccggagacac tctctgcgcc
       61 gaagaccatc ccgtcaccct ggagcgaatc gatttttacg agccggtgac ctcggtggcc
      121 atcgaaccga aatcccggca ggatcaggag aaactggact tcttcctgca gaaactgggc
      181 gaagaggacc cgaccttccg ggtgaagttc gatgaagaca cggggcagac catcatctcc
      241 ggaatgggtg agctgcacct ggacgtggtg gtgagacgcc tgctggaaga tttcaacatc
      301 tcggtgaatg tgggcaagcc gcaggtcgtt taccgggaga cgattacgga aagcgccgag
      361 ggcgagggga aattcgaacg ggagatcgaa ggggtcatga atttcggcca tgttcggctg
      421 cgcctggagc cccgtccgag gggtgcggga atcgagttca tcggaaattc ctcggatggc
      481 accatccccc cggaattcat cccgggcatc gaagccgggg tgaaggaatc gacggggaat
      541 ggatgtgtgg cggggtatcc ggtggttgac ctgaaagtca gcctgctggg ggggaccttc
      601 ctggaaggaa gttccactcc gcttgcctat cagatcgcct cggccatggc ttttcgcgac
      661 ggctgccaga aggcccagcc cgtcctgttg gagccgatca tgaggaccga agtggtggtc
      721 cccgaagagt tcgtgggtga ggtgatcggg gatttggggg cacgcaaagc ccgaatcgaa
      781 caagtgcaag ccaaggggaa agtcagtctc atagaagcct tcgtgcccct gaaagaaatg
      841 ttcgggtact ccactgacct gcgttccctg acccagggcc gggggacatt caccatgcag
      901 tttcaccggt tcgactgggc gccggagaag aagaaaagcg cataaggaat agagtaagga
      961 gcagagagaa aatacccaac tcggcggcat agccggaacc aatcggaata ttggaggggc
     1021 ggccccctgt ggccgcccgc aaacgcgttg ccaaaatcct ccccagcccc cctttaccaa
     1081 aggggggcga ggggggattt ctggggtgcg cgttgtctgg ggcatatcat atcatttggg
     1141 tacggctgcc caacactgtg tcctctttgc ctccgtggtg aaaaatcaat gaactatttt
     1201 tggcgtctgg actgccggta ttttatcggg gaccggccct gcaaattcaa acgggtctgc
     1261 gaaggatgcg agcagtattc acccatgggc caccgcatcc tgatcgtgaa actggcggcc
     1321 atcggggatg tcctccgtac cacccccctg ctcaccggat taaagagagt ctacccccgg
     1381 agccacatta cctgggttgt tgacaaagag gcctatcctc tcctgaagaa taatcgctgc
     1441 atcgaccggc tcctcccctt tgatttcgcc tccctcctgc ccctggaggc ggaaaccttt
     1501 gacctggtga tcgggctgga gaaggaaccc aggggggcgg cgattgtttc caaagtgagg
     1561 gcgaaagaga aaagagggtt tgccctggga ccggagggga atccttttcc cctgaatcct
     1621 tccagcgagt atgccttttt cctcggcttg tccgatgagc tgaagttcca tcagaaccgt
     1681 aagacctacc cggagctgat cttcgagatc gccgacctcg attatcaaaa ggatgactat
     1741 ctctttttcc tttcccccga agactccgct ttcgccgaga aatttgcccg aaagacgggc
     1801 ttaaaaaaaa gcgaccgggt gatcggtctc aacaccggtg ccggcgcggt ttttgccaac
     1861 aaggcctgga ccgaagaggg gtacctgggt cttaccaaac aattgacaaa ggaacccaga
     1921 accaaagtcc ttctcctggg cggccccggc gagaaggagc gaaaccggag gatcctaaaa
     1981 aaggcccggg gagcagtcat cgatacaagg tgcgacaaca ccttgggtca gttcgcagcc
     2041 atactgaatc tctgcgacct ggtagtaacg ggagatacca cggccatgca cctggccatc
     2101 ggtttgaaga aaaaagtcat cgctcttttc gggccgacct gcgcgcagga gattgaactc
     2161 tacgggagag gagaaaaagt ggtctcttcc atcccctgcg cgccctgcta tcggcgaaac
     2221 tgtgaggtct cccccaactg catggaagcc atcaccgccg aggaggtgat gaaaaagatc
     2281 aaaggcctgt tacccagatg acctccttcc tccgccgaca cctgaaattc attcttggcc
     2341 tggccatcag cgcaggcgcc ctttacttgg ccctcgatcg agtcgacttc aaggcgctag
     2401 gggactcctt ccagtcggcc aactatttct tcctggttcc tgccgggctc gttcaaatcg
     2461 ctgtcttctt cctcaaggcg gccggctggc gttatctgct catgcccgcc aagaaggaag
     2521 tccggctgaa atccacggtg accgtgctca tcatcggctt gatggtcaat aatctctttc
     2581 ccgccaagat gggagaactg gcccgggcct atttgatcgg agaaaaagag cgccttccga
     2641 agaccctatg cttttcaacc attatggtcg agcatctgct ggatgttctg gtgttaatga
     2701 tctttctcct agtcctcatg cccctggtcg cccttccccc ttggctccgg acgagcggga
     2761 ctctggtcgg ctttgcagcc ctgaccctga tcctggttct atttctcgtt gtccgccggg
     2821 aagagaagtt cctgggctgg atcagccgga tcctggtccg ggttccgcac cgtttccggg
     2881 ttaaaatcca ggaaatgctc cagaacgttt tgcaagggat gagagtggtc actggccgct
     2941 acatcgtata tgcttttgga tgcctgctgg tcatgtggac gacagtattc gtggtggctt
     3001 acctgctgat ggcggctttc ggcctcttcc tgccctttac cgcccccatc atggtgatta
     3061 ttttcaccgc cttcgggaaa atcattcctt cttcccctgg aggcatcggg actttgcact
     3121 acctgatcct ggtggttctc atgtcctttg gaatcagcaa ggaagtcgcc ctgggatgtg
     3181 ccattctcat gcacgggttc agcttcttat ttgaagtggg cgcggggttc gtggcgttgg
     3241 ttttcagcgg actttctctc ggaggggtga cccggcgggc cgaggagcaa gcatgaaagc
     3301 catggccatg attcccactt acaatgaggc cccgaacatc gaacccctcc tgcgggagat
     3361 tctgcaccag ggactggaca tcggcgcggt ggtggtggat gacaattccc cggacggaac
     3421 atgggccatc gtagagcgtc tccggaaaga agaccccagg gtccatctga ttcggcgcat
     3481 gaatgaacgc ggacggggaa ccgcgggagt ggccgggttc aaatatgccg tggcccaggg
     3541 cgtggatttc gtcatcgaga tggatgccga tttctcccac cagccttcct atatcccttt
     3601 tttccttagc cagatggaag actgcgacct gctgatcggc tcgcggttgg tggaaggagg
     3661 gggagaaaag ggaaggcact tcctgcggca atggattacc cgctcagcca atgcctacat
     3721 ccggctgatt cttggtcttt cagtgcggga ctgcacctcg ggctaccggg tctttcggag
     3781 gcaggtcctg gaggccatcg agctggacca actcatggcc gtaggtcctc ctatcgtgga
     3841 agaagtcctc tacaaagcct acaagcgagg cttccggatc aaggaagtcc cctatatcct
     3901 cgaagaacga aaagccggag agtctacctt caaccgcaag atcatgctca acgccctgct
     3961 gatgatgcca aagatccgct gggcgtaccg caagtatccc ttaaaagatt cctctatgtc
     4021 ttacaaataa attgcgggga aactgcaacc caaattccat cccttcctga ttgtaaaggc
     4081 aattcatgaa ttgcccctat ttgcttttcc gaaaaccttg tgataatctt tgcctgtctt
     4141 caccgaagga gggacgccca tgccagaggc taactccgca ccgatccttt ccaatccacc
     4201 catccgatac ccacagattg cgcctgagcc tggtcaagaa ccgaaactcc tggaccgcct
     4261 ccgcgaagcc ctgcactccc gccactacag cccaaaaccc gagtttcgcc ataggctttt
     4321 ccgtgaaact catgttgagc ttttgggacc agcgactccc cctcggagca tgtgtcgcaa
     4381 tataaaaagc gctgtcattc ccgcgaaagc gggaatccag ggaatccaaa ttcgaaccgt
     4441 tcaggaactt ctgggccata acgatgtgaa aacgacgatg atctataccc atgtacttaa
     4501 tcgtggcccg ggcgccgtgc gcagcccagc cgatggctta taaggtgaag aaagagggtt
     4561 tttataccga tccatataag aaaccatgat aaaggcgtta gggacttaac caccggatat
     4621 tttatgggta ttggaaaata atcgataggg tgttatacgg accggaaggc gatttccggg
     4681 atattacgcg gatccatcta tttactgtag aggcagaaat ataagatgag aaatacctta
     4741 gtcgtgctat ttctactcgc attatgtagt gttgtatgcg cagaaaccaa agtgaaaata
     4801 atgcccgacg agttgcttaa gatcgctgaa aaatgcgctt gcgagcaagt aaatgatttc
     4861 tataatcggc ctggtatgat taatccaccg tatgtttacg gcttcttgcc aggtgagcag
     4921 gaaaatagcg ccgttttctg gtgccaagaa caccatgcga acccaaaata tctattggtc
     4981 ttagcatcta gaaagacttt ttttgatgat tggaactacg aaattatcat taaaacagag
     5041 aattatcctc gtggtttatc catcgttaaa aactgtaaaa ttacattgga taaattctat
     5101 tatatctcta gtccaaaaaa acggggtccc aacggcgttt tgccagttgc gaacgtaata
     5161 ttgagcactt atgatggagt ctcagagtat tattataatc ataagggttc ctggttaatt
     5221 tctatttggc actgattagc ctctaaccac cgcatgaacc tgacgcggaa tagctgcgtc
     5281 agattttagg cgttgctggt tgcccgcgca ggttatgcga atcgttcgac cgcaagagga
     5341 gggacctgct gatgcccaac gcacagcagc gcttccgaca ggtgaatgcc acgcgctatc
     5401 gacttggtca gcaagctggc cattacgaga gcttcttcca acgcgcgaat cacccgagcc
     5461 gtcctctggc attctggatt cggtacacga ttttcagccc ccagggccgt cccgaagacg
     5521 ccgtgggcga gctatgggcg atctacttcg acggcgagac tcagcaccat gtcgccgtca
     5581 agaccgaggt cccactcagc gagtgcacct tcaagacgga cgactttctc gtcctcgttg
     5641 gtaacgcgcg cctgggcccc gggcacgtcg ccgggtcagt gacctctggc gctcacgccg
     5701 tgtcctggac tctcgacttc catggtgacg ccgagccgct cttcctgttg cccatcgaga
     5761 tgtacgacag ccccttgctg ccggccaagg gccttgtcgg cctcccgctg gccgtctaca
     5821 acggggcttt gctcgttgac ggccgtgaga tcaccgtcac ggactgggtc gggagccaga
     5881 atcacaactg gggcctgaag cacacagacc attatgcctg ggggcaggtc gccggtttcg
     5941 acgcccaacc cgaaacgttt ctggaggtcg cgacggcccg tcgcaggatc gacactgact
     6001 ggagcccgtt catgacgccc gtcgtactgc gtcaccgcgg acgcgagatc tccttgaata
     6061 ccgtggccca aatggtccgt gccgaggcgg ccttctccta ttccatgtgg accttcaagt
     6121 cggaaacgga cgaaatcctg ctcgagggaa cttttgccgc accccgcgag gccttcgtcg
     6181 gccttggcta tcgaaatccg cccggcggca ccaaggagtg cctgaacacc aagatcgcat
     6241 cgtgcgagct gcgagtgacg caccgacagc cgggcggagc gacacgaacc gagactttct
     6301 cagcgaggaa tcgcgctgca ttcgagatac tcaccgacga acacgaccac ggtgtagaaa
     6361 tccgcgcttg agatgagatg tataggggga cgttgttcaa aacattcaaa ataattgttt
     6421 acatgccatt gggattatga taatggacaa ttatgccacg aggagctcgg cttgatgtcc
     6481 ctggaaccct gcatcatgta atgatccgag ggatcgaggg aagagagata tttcatgatg
     6541 acgaagatcg ggagaatttc atttcccggg tcgagactct ggtgaagacg actggagacc
     6601 gaatcctggc ctggtcgcta atgaagaatc atgtgcattt tttgctcttt agcggaccgc
     6661 tggggcttcc gacctttatg agaaggctcc tgactgggta tgcggtgtgg tttaaccgga
     6721 ggcacaggag ggtggggcat ttattccaaa accggtataa atcgatcgtc tgtgaggaag
     6781 agccatattt attggaattg gtgcggtata ttcacttgaa tccattgagg tcccgagcag
     6841 taaagacttt gtcggaattg gataaatact gctggagtgg ccaccgtgta ctggtaggca
     6901 aagggcggaa tgattggcag gagaaagcct acatcctatc ccaattcggt aagagagaaa
     6961 ggggggctat acgggcctac cggaaattca tggaggaagg gatcaatttg ggtaggcggc
     7021 cagagttggt cgggggagga ctcattcgaa gtcggggagg ttggtcaagg gtgctatccc
     7081 aaagggctaa gggggagaag gaagaatatg attcccgaat cttggggagt ggggattttg
     7141 tcctagatat aatccgagac gcggatcaaa agatgcgtcg ccagattaga gggaggggag
     7201 aaaatacttc gattgaagaa at
//
LOCUS       DASPRR010000267        18614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1350747, whole genome shotgun sequence.
ACCESSION   DASPRR010000267 DASPRR010000000
VERSION     DASPRR010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18614)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18614
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350747"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="VEL68_10415"
     CDS             <1..109
                     /locus_tag="VEL68_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXZ36421.1"
                     /translation="EMKDGKSEPVNMDECLGCESCVEVCPTGAITVKEV"
     gene            623..1828
                     /locus_tag="VEL68_10420"
     CDS             623..1828
                     /locus_tag="VEL68_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ36422.1"
                     /translation="MAKSKVSIVKTNPQPYYSAIRKAVEKALELIGGVDDLIKPGEKV
                     LLNPSWVAPPTEPEKGCITQAEVTQAVADIVREKGARAIIAESSAVGVDCQKVIDGSG
                     YRVLKEKGYEIIDLKKSETAMMKVPHSVVFPEIESHRIVQEADVIISLPKLKTHDQTE
                     ITCSIKKLKGLLSDKYKRLMHQEGLFEGVVDLLSTLKPHLAIVDGIYCQEGLGPVFGK
                     PVEMDLILAGRDLVAVDAICGVVIGFAPEEVLMTQTAAKRGMGTAKLAAIEVLGEPVE
                     KVRRRFLRSVEDDPVKVEGFNLIFGGITCTGCRNTVVSALVDMRNADQLMYLPGVTVV
                     TGDPEVPPFVSPGSIVTVGKCVPEGKRGKVHVKGCPPNNAFVVQAIMGDRAKAKRMYA
                     DGDLDSTER"
     gene            1937..3118
                     /locus_tag="VEL68_10425"
     CDS             1937..3118
                     /locus_tag="VEL68_10425"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXZ36423.1"
                     /translation="MAKVAKQAVQVPYSRIRALAEEAMKMEGVVKLYFGESNIPTPDF
                     IKQAARRALEEGYTFYTSNAGLPGLRADLAAYYRRLQGVELDPESEIVITASGVQALN
                     LTIRLAIDPGDEAILLTPAWPNATSTVLLDSGTPKEIPLRLRGGRYRVDFEALEEAVN
                     SRTRLLVYTSPSNPLGWVATEEEQEGILNFARRHDLWVLADEVYDRIYYRAPEVGMPA
                     PSILRKATRDDAVMVAQSFSKTYCMTGWRVGWIVARRDAAGKAAQLNEFIVSHAASFT
                     QRAAQAALTHGEEGIREMLVRLRENRDFCLRALSEIRGVSVPSPDGAFYLFPKIEGLK
                     DSFEFCLSLLRETKVGIAPGSAFGESGEGSVRICYAAERPILEAGMEKFSHYLTSSWA
                     G"
     gene            3240..5141
                     /locus_tag="VEL68_10430"
     CDS             3240..5141
                     /locus_tag="VEL68_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ36424.1"
                     /translation="MPKAYNGKILRVDLTTGNIQVEEPGEVIYRTYFGGGGLSSYYLL
                     KELKPGVDPLGPENILIFASTACTGIPISGWVRFTVAAKSPLTGGYGEAEAGGFFGPE
                     LKFAGFDAVIIKGKSPRPCYLWIHDGQAEVRPAGAIWGLETGPAQERIREEVGELRAR
                     VAQCGPAGEKLVRYACVVNELKHANGRTGMGAVMGSKNLRAIAAKGTQRLEVHDPERM
                     KELAKLQSELIASWGPSKVMRSLGTPNLIMPLNTAGILPTRNFRSGSFEGAENICGEK
                     MRETLLKAEEGCYACAVRCKRAVEVSSGPYTTSPQYGGPEYETLGSLGSLLCIGDLSA
                     VSKGNELCNRYGLDTISVGVAIALAMECYENGILTKADTGGLDFRFGNADAMIQAIHW
                     IAKREKGLGDTLAEGVKRAALKIGKGAEKYAMEIKGQELPMHDPRGKTGQGLSFALSP
                     TGADHVEAPHDTPFAQPGPMMGKIAPLGLLEPVPARENGPRKVRNFVYLQYLWSMFNS
                     VGICNFTAGPVWSLPLNKWPEVVYAATGWESSLFELCKLGERTVTMARVFNLREGFGR
                     KDDTLPDRIFEVLEGGALAGKGIDRKEFDEMLTLYYEAMGWDPDEGVPTRGKLAELNL
                     YWLDEYLKR"
     gene            5477..6550
                     /locus_tag="VEL68_10435"
     CDS             5477..6550
                     /locus_tag="VEL68_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36425.1"
                     /translation="MAPVGQLPAVVVSVTPSIYALPILLVEKAGEWKDFGIQARIKVY
                     AHGEEQLDRSAANEWEVGIMDPLCAVRGGNEGNVAIVGIGGNFASQVTFFPSQRGASP
                     QPGKWGEWLKDYVARMESKGTKPFLPACLVATSSYADTRKTLVIRWLEGYSRGIQIIK
                     KNPEEAASQLQKFYRETLKREVQPEFLRQEIRDAFFFDEKDREEPFRRKGGRPSLIDE
                     FTSSMTQYLKGVKALNTQVEPSDYVLSMLCDQLSALRGEAVAQLEKTKGSIEAAAQAG
                     SPVDDFRKTWEESKTQIQEGRGCLAVIGVLSNLQRSADQARMASARLSDFRRIEMAVG
                     GMLGLYYAGYFFHRRKKAKARSS"
     gene            6734..7381
                     /locus_tag="VEL68_10440"
     CDS             6734..7381
                     /locus_tag="VEL68_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36426.1"
                     /translation="MIDFRSQSLRIKYFAVPFLILSILFLWNQWRISNPDTALNTARE
                     YLEGQFPGDKWDLERLALVGEGLGKKWRLEFSNLQKNGRRIQANLLVDRWMPGKILGR
                     FVIILSPPQVLDGEWSKPALWQQISSKVSRQGFFFIGILLLALQIFWLYRMHRREVFR
                     KPDGAVLAFLGAALLLIFALTEVHPGFMIAYPLTLTFLGLAAGFTAGKTDVPQKE"
     gene            7362..8162
                     /locus_tag="VEL68_10445"
     CDS             7362..8162
                     /locus_tag="VEL68_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36427.1"
                     /translation="MSLKKNSKVSRTVILAAVSMIAVIGVWLFLTSVLRIIPPAFLPP
                     PQNIVTDFLRILESPYVGHTLVGHTLVSLQIVLGGFFSALLIGIPLGILMGWFKRIEA
                     VVDPLFQLLRPIPPLAWIPISLLWFGIGVQSKIFVIWLSAFVPCVINSYTGIRLTDPL
                     LVKAALGLGVKRQQDLLREVAIPSALPIILGGVRIGLGSAWMTLVAAELLASDAGLGY
                     MMQMARRALEPSVIILGMLVIGILGALMARGLRALENRVCPWVKEEEH"
     gene            8167..8967
                     /locus_tag="VEL68_10450"
     CDS             8167..8967
                     /locus_tag="VEL68_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36428.1"
                     /translation="MARYWESLGEKIYQEKIILSISSVLGFLFLWYLITDLFGFMPAV
                     VLPSPEKVFFTIYEKISVPVGEEKLFGHIGVSLVRILVGVGIALGLAIPLGILMGWYE
                     DLDSLASPIIEVLRPIPPIAWIPLAILWFGIGLGSKVFIIAIGVFFPTLINTHIGVKF
                     VDPLLIKAAQTLGAKDKNILWEVVVPASIPLIVAGVRIGVGLGMMCLVAAELVAASTG
                     LGYLIMLGGDDLKPELSITGMILIGILGLIADRIILGVERRVIYWKKE"
     gene            8975..9790
                     /locus_tag="VEL68_10455"
     CDS             8975..9790
                     /locus_tag="VEL68_10455"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004592796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36429.1"
                     /translation="MNEEQRSDKLSAQNVSVMFEKDDKSFLAVDDVSLTIQEGEFVVI
                     VGPSGCGKTTFMNTFAGLVRPGKGKVFMDGEEITGPSAERGVVFQQFALFPWKTVKEN
                     IEFGLKYRGIPKEQRDETIRKYVQLIKLEGFEKSYPHELSGGMKQRVAIARVYANRPQ
                     VLLMDEPFGALDAQTRTIMQEDLSEMFLTEKRTVLFITHSVDEAIYLGDRVVIMTARP
                     GKIKKIIDITRDNLIPDRRRMRYDEVVSLKEFLDLKGEIWGLIKAEILEKHIL"
     gene            9903..10949
                     /locus_tag="VEL68_10460"
     CDS             9903..10949
                     /locus_tag="VEL68_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36430.1"
                     /translation="MKGNWFMKLGMFSLAAAMILAMSTAAHSQQKELPLVRASHQPCM
                     HALPTILASTFNWWDQIGIRVSFHYFVSGMPQVEAGQAGEWDVGAIGTVPCLFAGIKY
                     KQPLIAISNDESVTNNLMVRPDAFNTWLKDPKKDLTGKTFLVSTISTGHYAVVAYLKK
                     IGLKESDVKIVHMEQSAILSAFLSGQGDVAQTWAPFNYMMEEKGMKVLSHGKDAGVVI
                     PGGVVATKKFADEKPELVAKWLEGYMRGIDFMIDNPRESAKHLGKYFREKCGINLSDE
                     VCLKEFELRPLFRTKQQIRMFDRKGGKMSTVDKWMDDLAQYFVEVKKIDAKPDPKSYI
                     TDKFLQMVDKQAKK"
     gene            11146..12483
                     /locus_tag="VEL68_10465"
     CDS             11146..12483
                     /locus_tag="VEL68_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXZ36431.1"
                     /translation="MGKEVTRRGFLKGMAAAGAVALTAGVAPKAFGQAPAKGGFNWKK
                     FAGAKIRFMNNTHDWSTELIPKALPEFEKLTGIKVQWEVLAENQFRQKLTLELSSNPD
                     SVDGFMSLPSWDGMAFYQSGWYEPVEKFLKSPDLTSPDFDFKDFFPSCTQIATIKNTL
                     VGVPLYPEVQMLYYPKEKFQEKKLKVPETFEEFAATAEKLYDKNTKYAGYVSRGDGIQ
                     AVYTLAPFVFGMGGRWLDENGRPDLTSDAFVKALELYSGTLHKYGPPGIAGQTWAQNQ
                     VVFMQGNAGMNTDSSNFISTYEDPKRSKLAGKVGYAPVPRGPAGLRSSLIAWCLSIGS
                     RSKNKEATWMFIQWLASKEMVLRQAKMKLPVGRESAWKSPEFQSSMSKEWLDAFTKEL
                     PTAAVNQANPLVVQVPETRDAIGKAIVSVIQGGNAKEAAMKAQQDCLKILKMT"
     gene            12944..13762
                     /locus_tag="VEL68_10470"
     CDS             12944..13762
                     /locus_tag="VEL68_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ36432.1"
                     /translation="MIVTVLLMVFPLFYLLYMSVHDWLLIGGKPPQFIGFQNYIALFQ
                     DERFLASLWRIGIFIALGLLVQIPVGLTIAVLFNERFPGRGIMRTLLLLPMVATPVAM
                     ALIWVIMMEPSSGVLNYFLSWFGIGAVAWLSDVNLVIPALVLVDTWQWTPMVALICLA
                     GLSALPMEPFEAARIDGANVWHRFWKLTLPMVWPTLLIAILFRFIDLLKVIEHIYVMT
                     RGGPGFASETINLYNFLMGLFYYRVGYGSSISVVIFAMVLGASLMLIRARRTSW"
     gene            13756..14607
                     /locus_tag="VEL68_10475"
     CDS             13756..14607
                     /locus_tag="VEL68_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ36433.1"
                     /translation="MVGSGRVAGSPVRHNPAFRKAVLLVLGILVLIPFLFPFFWMLLG
                     SVKTEVDFMAYPPVWLFKPTLENYVKVFVENDFGTFSLNSTIVAAFTTFFALLFGIPG
                     AYAVARYKRTGFALIVLAARMAPGIAFLLPWFVLFVQLRLTNTYFALVTSHLIFTLPL
                     VLWMMIGFIEAIPKEIEQAAMVDGCTVWGSLFRIIVPLTLPGVAASGILCFIFSWNNF
                     LFSVVLAGEATKTLPVAVFNFLGYQSIAWGPLSAAASIITLPVLIMALIVQKYIVQGL
                     AAGGITG"
     gene            14681..15643
                     /locus_tag="VEL68_10480"
     CDS             14681..15643
                     /locus_tag="VEL68_10480"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXZ36434.1"
                     /translation="MKNIPYARVSHTVIEDVGQVKPAENVLILTDTEVSQTIPTSLAE
                     AVHAARGIPSILVMAPPEYPGCPLPAPVEFAVQGAQVVIMATSKSVAHAEIFGREMKK
                     RRLISMPGIMERSFLDGGGTADLKELKEITERVSRALEGKRRFAIKSDKGTDAAVFSR
                     GKAFSFYAQPPSPVGFAMFPDGESVVALEEKTLEGRIVMDVFQTGVGPLREPIAFEMK
                     NGKAVRITGGIEAQLLGEVLEKYGDDNSYYFGEFALGTNPKARFIGSAGEDKKRIGTV
                     HMSLGDDIGIGGELKSKLHLDAVMGRPTLVADGQILIEDGRLLV"
     gene            15726..16325
                     /locus_tag="VEL68_10485"
     CDS             15726..16325
                     /locus_tag="VEL68_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXZ36435.1"
                     /translation="MKRKVPYKKLIEDFLLISHRLRQRELTFGGGGGISIQIPRSRKV
                     LIKGWEVASEDAGKKDISLVDLDGKQLNQVKPCLETPLHLEVLKVRKDIRAVIHAHSP
                     YLTSFGNIKIRLPKADLKNYTFLGVTTAFAPYADPGSKELAELVSKPFQKEEVFCVLM
                     EDHGVTVVGTDIFNAYYRLDILEGMAKTFILTKLIRQII"
     gene            16406..17350
                     /locus_tag="VEL68_10490"
     CDS             16406..17350
                     /locus_tag="VEL68_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36436.1"
                     /translation="MNAKEIKKVTLIGSGTMGPGIAQTFARAGLEVFLVDIKQEILDR
                     GMDRIGDNLDVFIELEVIKPGEKEAILSRIHPTTDMEFACRQADYFMEAVQEILERKQ
                     QVHRQADHWCRPDVIFTTNASNMQMNPIASVTKRPDRFVGTHWVNPPHIMQLVEVVRG
                     PLASEAAVETVYQFLKRVGKAPVICKDTLSYINNYVQSALGRAALELLEQGVATAEDI
                     DQAVNTGFGFRLPVVGPLAFFDMAGLDNVRDSWEYINKMGGKMGPLPHFILDLIAQGH
                     WGLKTGRGIYSYSEQEIRELAKERNRQLIRMLKALQRI"
     gene            17400..18449
                     /locus_tag="VEL68_10495"
     CDS             17400..18449
                     /locus_tag="VEL68_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ36437.1"
                     /translation="MEEEKAAWACALQEQGFFPGSCTVSTAGRKAPACQATLRKKNDH
                     FERLISAVHLSRPENYLSIYQSGCNFSCRKCHSWYFSKKAEGKWHTPKEILQKCIEYD
                     QKVNLNEPRFKATAYHAHDTCRCCGSCVLHGKQSSLCPGILEPSAIVLSPQGFGPVRN
                     IVGFTGGDLTCQPKFYARCSELIKSHTRLWVLIETNGYGLTPNHLDLLRSAGVDAYWL
                     DIKAFREDTHHWLTGCTNEWILKLPEEMKKRDFTVEVLSLYIPQVVETDQLEAIAAHL
                     ARIDRDIPFTILAFFPEYQMKEYRPPTLEEMVGAFQAVEGAGLKNVRLGNTGIFAHTE
                     DQIQTLRQKVRMGHF"
BASE COUNT         4272 a         4827 c         5374 g         4141 t
ORIGIN      
        1 tgagatgaag gacgggaaat ccgaacccgt gaacatggat gaatgcctgg gctgcgagag
       61 ttgcgtcgaa gtctgtccca ccggcgccat caccgtaaag gaagtttagg gttagaccca
      121 gggcccgaat aaaggatgtt ccgaatcggg aagaggcgat gagatttgtt tccatcgcct
      181 ctttttttaa atcgaatgcc atttcaccgc cttttcgaag acttagatac ccttttaaat
      241 tgacaccccc agagctctgt ggtatggtgc cgtttattgt atctagccaa ttctcttcga
      301 tccaacatga aattcaaccc tatcctccag gtggccccaa ggtacgaaga taccggctag
      361 gatgtctcaa caaaaatgca ctccccaaaa ttcatttcct tgagggaatc ggaataatca
      421 ttcgggcagc cgcccttcaa aagggatttc agagggcgca tttgagcttt ggagaccctt
      481 tcccattaaa cccagaaatt tctgatcggg gaattcgcaa tgctggtttc ggcacccacc
      541 aaaccataag aatccagcct ttcccatttg gcataggata gaggggtgag gggatccttt
      601 tccagctgac gaggaaattg gtatggccaa aagcaaggta tcgattgtca aaacaaatcc
      661 ccagccgtat tattcggcca ttcgcaaggc ggtcgaaaag gccctggaat tgattggggg
      721 cgtggatgat ctcatcaagc ccggggaaaa agtccttttg aaccccagct gggtagcgcc
      781 ccccacggag ccggagaaag ggtgcatcac ccaggcggag gtgacacagg cggtcgccga
      841 catcgtccgg gagaaagggg cccgggcgat tatcgccgaa tcctcggcgg tgggcgtcga
      901 ctgccagaag gttatcgacg ggtccgggta ccgggtgttg aaagaaaaag gatatgaaat
      961 catagacctg aagaaaagcg aaacggcgat gatgaaggtc ccccatagcg tcgttttccc
     1021 ggaaatcgaa agccaccgga tcgttcagga agccgatgtg atcatttccc tgcccaagct
     1081 aaaaacccac gaccagacgg agatcacctg ctccatcaaa aagttgaagg ggctcctttc
     1141 ggacaaatac aaacggctga tgcaccagga ggggttattc gagggagtcg tggacctgct
     1201 ttcgacgttg aagccgcacc tggccatcgt tgacgggatt tattgccagg aagggctggg
     1261 gcccgtgttc ggaaagccgg tggaaatgga tttaatcctg gcgggaaggg acctggtggc
     1321 agtggacgca atctgcggcg tggtcatcgg tttcgccccg gaagaagtcc ttatgaccca
     1381 aacggcagcc aagcggggaa tgggaacggc gaaattggcg gcgatcgagg tcctggggga
     1441 accggtcgaa aaggtccgac ggcgctttct tcgttccgtc gaagacgacc cggtcaaagt
     1501 ggagggtttc aatctgattt tcggtggaat cacctgcacg ggctgtcgga acacggttgt
     1561 gagcgcgctg gtggatatgc gcaatgcgga ccagctaatg taccttcccg gtgtgacggt
     1621 agtcacaggg gacccggagg tcccgccctt cgtttcaccc ggctcgatcg ttaccgtggg
     1681 aaagtgcgtt ccagaaggaa agcggggaaa ggtccatgtc aagggctgtc ctccgaataa
     1741 cgctttcgtg gtccaggcaa tcatggggga ccgcgccaag gcaaaaagga tgtacgcgga
     1801 cggagatttg gattccactg aaagatgaat cggcggagga gtcggaaccc tctccggatt
     1861 catgatttcc tcgatccttc ctaattctca agaggggcgg gaaagaccta ttccttttga
     1921 aaaaggcagg ccgaaaatgg cgaaagttgc taagcaagca gtgcaggtcc cttactctcg
     1981 gatccgggcg ctggccgaag aggctatgaa aatggaggga gttgtcaagc tatatttcgg
     2041 ggaatccaac attcccaccc cggatttcat caagcaggcc gcacgccggg ccctggaaga
     2101 agggtatact ttttatacga gcaatgcggg acttcccggt ttgcgtgcag atttggctgc
     2161 ctactaccgt cggctgcagg gagtggaact ggaccccgaa agcgagattg tgatcaccgc
     2221 ttctggggtc caggccttga acttgacgat ccgactggcc atcgacccgg gggacgaggc
     2281 cattttgctg actccggctt ggccgaatgc gacctcgacg gtgctcttgg acagcgggac
     2341 gccgaaagaa attcctctcc ggcttcgggg tggccgctac cgggttgatt ttgaagccct
     2401 cgaggaagcg gtaaattcgc gaacccgcct tttggtatac acttccccct cgaatcccct
     2461 gggttgggtg gccacggagg aagagcaaga ggggatactg aatttcgccc gccgccacga
     2521 tctctgggtg ctagccgatg aagtgtatga ccgcatttac taccgtgcgc cggaagtggg
     2581 gatgcctgcg ccctcgatcc tgcggaaagc cactcgggac gacgcggtga tggtggccca
     2641 gtctttttcc aagacttact gcatgaccgg ttggcgggta gggtggatcg tcgcccgccg
     2701 ggacgcggcg ggaaaggccg ctcagctcaa cgaattcatc gtttcccatg cagcgagctt
     2761 cacccagcgg gcggcccagg ccgccttgac ccatggggag gaagggatcc gggagatgct
     2821 tgtccgtctg agggagaacc gggatttctg cctgcgggcc ttgagcgaaa tccggggcgt
     2881 ttcggtccct tccccggacg gtgccttcta ccttttcccg aaaatcgagg gcctgaagga
     2941 ctcctttgaa ttttgcctct ctctccttag ggaaacgaag gtgggcattg cccccggaag
     3001 tgctttcggc gagagcggag agggctccgt ccgcatctgc tatgcggccg agcgtcccat
     3061 cttggaagca ggaatggaaa aattttccca ttacctcacc tcctcctggg ccgggtagaa
     3121 gggcaaattc ccaaataaat ctcatttgtc gatcgccaag agctgcccgc taattgagtc
     3181 ttctttttgc tggcaatatc cacagacagg gatcactaat aaattaagga gggtttttca
     3241 tgccgaaagc ttacaatggc aaaattctga gagtcgattt gaccacaggc aatatccagg
     3301 tggaagagcc cggagaggtc atctaccgga catattttgg ggggggaggg ttatcctcct
     3361 attacttgct aaaggaactg aagccgggcg tcgaccccct agggccggag aacatcctca
     3421 ttttcgcttc cacggcttgt acgggaattc caatttctgg ctgggtgcgg ttcacagtcg
     3481 ccgccaaatc ccccctaacc ggaggctacg gcgaggcgga agccggcgga tttttcgggc
     3541 cggagctgaa atttgcggga tttgacgcgg taatcatcaa aggaaaatcg cccagaccct
     3601 gctacctgtg gattcatgat ggacaggccg aggtccgccc cgcgggggcg atctgggggc
     3661 tggaaacggg cccagctcag gaaaggatcc gtgaggaagt gggcgaactc cgtgccaggg
     3721 tagcccaatg cgggccggcg ggcgaaaaac tggttcgtta cgcctgtgtc gtcaacgagt
     3781 tgaagcatgc caacgggcgg acgggcatgg gcgcggtcat gggatccaag aacctgcggg
     3841 ccattgccgc caagggaacc cagcggctgg aagtgcacga cccggaaagg atgaaagagc
     3901 tggcgaagct gcagtccgaa ctgatcgcct cctgggggcc gagcaaagtc atgcggagtc
     3961 tggggacgcc caatttaatc atgccgctca acaccgccgg gattctgcca accaggaatt
     4021 tccgttccgg ctctttcgaa ggagccgaaa atatctgcgg cgaaaagatg cgggagaccc
     4081 ttctgaaggc agaggaggga tgctatgcct gcgccgtccg ttgcaaacgg gccgtggagg
     4141 tgagctcggg tccctacacc acttcccctc aatacggcgg cccggagtat gaaaccctcg
     4201 ggtccctggg gtcgctcctt tgcatcggtg accttagcgc cgtatcaaag ggaaacgagc
     4261 tctgcaaccg gtatgggctg gacaccatct ccgtcggcgt ggcgatcgcc ctagccatgg
     4321 agtgctatga aaacgggatt ctgacgaagg ccgataccgg cggtttggac ttccgcttcg
     4381 gaaacgcaga cgcgatgatc caggcgattc actggatcgc caagcgggaa aagggcctcg
     4441 gcgatacctt ggccgaggga gtcaagaggg cggcgctgaa aatcggcaaa ggggcggaga
     4501 aatacgccat ggagatcaaa gggcaagaat tgcccatgca cgatccgcga ggcaagaccg
     4561 gccagggcct tagctttgcc ctgtcgccaa caggggcgga ccacgtcgag gctccccatg
     4621 acacgccttt cgcacagccc gggccgatga tggggaagat cgctcccctc gggcttctcg
     4681 aacccgtgcc cgcaagggaa aacggaccga ggaaagtccg caatttcgtc tatctccagt
     4741 atctctggtc catgttcaac tccgtgggga tctgcaattt cacggccggt ccggtgtggt
     4801 ctctgcccct taacaaatgg ccggaagtgg tctatgccgc caccggttgg gaatcaagcc
     4861 tgtttgaact ctgcaagctg ggagaaagaa ccgtcaccat ggcccgggtt ttcaatttga
     4921 gggagggttt cggccgcaag gatgatacgc tgcccgaccg catcttcgag gtcctggaag
     4981 gcggagctct ggccgggaag gggatcgacc ggaaggaatt cgatgagatg ctgaccttgt
     5041 attacgaagc catgggttgg gaccccgacg agggtgttcc cacgaggggt aagctggccg
     5101 aattgaatct gtactggctg gatgaatatt taaaaagata agagggcaga ggtaatagat
     5161 cagaggccag agaattcccc ctacctcctc ttcccaacaa ggcggagggg gtgatgttgg
     5221 gaaaggatgg gctgctgagc ctgatgaagt ccgggtccac gtacatcgat gtccggatag
     5281 cggataccgg gtcctgggtc cctgatgtct gaaggatggc tttgtgatcg aaaattttgg
     5341 aaaagggtat gggcaattca tgaattgccc tacgatagag aggaaattgc gaagcccctc
     5401 tgcttataaa tcggccattc tttcctccgc gattttgggg ctgtttctgg gcttcccttc
     5461 ccccgggagg gcagcggtgg cgccagtggg gcagctgccg gcggtggtcg tgagcgtcac
     5521 tccctcgatc tacgcactgc caatcctgtt ggtggagaaa gcgggggaat ggaaggattt
     5581 tgggattcag gccaggatca aggtctatgc ccatggggag gaacagctag accggagcgc
     5641 ggccaatgag tgggaagtcg ggattatgga ccccttatgc gcagtaaggg ggggaaatga
     5701 gggaaatgtg gcgatcgtgg ggatcggggg gaattttgcc agccaggtca ccttcttccc
     5761 cagtcaaagg ggggcttccc ctcaacctgg taagtgggga gaatggctga aggactatgt
     5821 cgccaggatg gaatccaagg ggacaaaacc attcctccct gcctgcctgg tggccacctc
     5881 aagctatgcg gacacccgga aaaccctggt gattcgatgg ctagaaggtt attcccgggg
     5941 gattcagatc atcaagaaaa acccggaaga ggctgcttcc cagcttcaaa aattttatag
     6001 agaaaccctg aagagagagg tccaaccgga gttccttcgg caggagatcc gggatgcctt
     6061 cttcttcgat gaaaaggacc gggaggagcc ttttcgcaga aaagggggga gaccgagcct
     6121 tatcgacgaa ttcaccagtt ctatgaccca atatttaaaa ggagtgaaag ctctgaatac
     6181 gcaggtggag ccttcggact atgtcctctc catgctctgc gatcaactct ccgcccttcg
     6241 cggagaagct gtggcccaac tggagaaaac caagggctcc atcgaggcag cggcgcaggc
     6301 gggatccccg gtggacgatt tccgtaagac atgggaagag tcgaaaaccc aaattcaaga
     6361 aggacgggga tgcctggcag tgatcggagt tctctcgaac ctccagcgca gcgccgacca
     6421 ggcccggatg gccagcgccc ggctcagtga tttccggcgg attgaaatgg cagtgggcgg
     6481 aatgctcggg ctctattatg caggctattt ttttcatcga agaaaaaagg ccaaagcccg
     6541 tagctcatag cttccagtcc cctgttagcc aaaaggcgca gggaggaagg ggcgggagga
     6601 aggggcaaaa aaaagaagcg aagagctgtc tcaatggagt aagatttctt tgcgaagcgc
     6661 aagggaaaac aagcaaggaa ccacggaccc ctgacaacgg accatttacg ctggacccga
     6721 aacctttttg ggaatgatcg actttagaag ccaatcccta agaatcaaat actttgccgt
     6781 tccgtttcta atcctttcca ttctcttttt gtggaatcaa tggcgaattt ccaatcccga
     6841 tacggccctg aacacggcaa gagaatacct ggaagggcaa tttcctgggg acaaatggga
     6901 ccttgaaagg ctagccctgg tgggagaggg cttggggaaa aagtggaggt tggaattcag
     6961 caacctccag aaaaacggga ggagaattca ggccaatctc ctggtagacc gttggatgcc
     7021 gggcaagatc ttgggaagat tcgtaatcat tctgagccct ccgcaggtcc tcgacggaga
     7081 atggagcaaa ccggctcttt ggcagcagat ttcctccaag gtttcccggc agggcttttt
     7141 tttcatcggc atcttgcttc tggctctgca aattttctgg ctttaccgga tgcatcgccg
     7201 agaggtcttc cggaaacccg acggagcggt cctggccttc ctgggggccg ctcttctcct
     7261 gatcttcgcg ctgacggagg ttcatccggg gttcatgatc gcttatccgt taacccttac
     7321 tttcctgggg ctggcggccg gatttacggc agggaagaca gatgtccctc aaaaagaata
     7381 gcaaagtttc ccgtacagtg atcctcgccg cggtttcgat gatcgccgta atcggggtgt
     7441 ggcttttcct gacctctgtc ctcagaataa ttcccccggc ttttctccct ccgccccaga
     7501 acatcgtgac cgactttctg cggatcctgg aaagtcccta tgtgggccac accctggtcg
     7561 gacatacgct ggtaagcctg cagatcgttc tgggcgggtt tttctcggca ttgctcatcg
     7621 ggatccccct gggaatccta atgggatggt ttaagcggat cgaggcggtc gtggaccccc
     7681 ttttccagct ccttcgcccc attccccccc tggcttggat tcccatttcc ctgctgtggt
     7741 tcggaatcgg ggtccaatcc aagattttcg tcatctggct cagcgcattc gtgccgtgcg
     7801 tgatcaactc ttatacagga atccgtctga ccgatcccct cctagtcaag gccgcgctgg
     7861 ggctgggagt gaagcggcag caggaccttc tccgcgaggt ggccatcccg tcggcccttc
     7921 ccattattct ggggggggtg cgaatcggtc tggggtcggc ctggatgacc ctggttgccg
     7981 ccgaactcct ggcctcggat gccggcctgg gatacatgat gcagatggcc cgaagagcgt
     8041 tggagccctc ggtcatcatc ctgggaatgc tggtgatcgg aatcctgggg gcgctgatgg
     8101 cccgggggtt gcgggccctg gagaacaggg tctgcccctg ggtaaaggaa gaggagcact
     8161 gattccatgg cccgttattg ggagagtctc ggagaaaaaa tataccagga aaagatcatt
     8221 ctttccattt cctcagtcct ggggttttta ttcctttggt atttgattac cgatcttttc
     8281 ggcttcatgc cggccgtcgt actgccctca ccggaaaagg ttttctttac catttacgag
     8341 aaaatttcag tcccggtggg agaggagaag cttttcggcc atatcggagt aagcttggtc
     8401 cgaattttgg tcggggtggg gatcgccctc ggcctggcca tccccctggg aatcctcatg
     8461 gggtggtatg aggacctgga ttctctggcc tctcccatca ttgaggtgtt gcgccctatc
     8521 cccccaatcg cctggatccc cctagcgatt ctctggttcg gaatcggcct ggggtccaag
     8581 gttttcatca tcgccatcgg ggtcttcttc cccaccctga tcaataccca tattggggtg
     8641 aaatttgtcg accccttgct catcaaagcc gcccaaacct tgggcgccaa agataagaac
     8701 atcctctggg aggttgtggt cccggcatcc atccccctca ttgtagccgg ggtacggatt
     8761 ggggtcggtc tgggtatgat gtgcctggtg gccgcagaac tggtggccgc ctccacggga
     8821 ttgggatacc tgatcatgct gggaggggat gatctgaaac ccgagctctc tatcactgga
     8881 atgatcctga tcggcatcct cgggctaatt gccgaccgga tcattctggg tgtcgagcgc
     8941 agggtgatct actggaaaaa ggagtaatgg atcggtgaac gaagagcaaa ggtccgacaa
     9001 attgtctgcc cagaacgtgt cggtcatgtt cgagaaggat gataaaagct tcctggctgt
     9061 ggatgacgtg agcctcacca tccaggaagg cgaattcgtg gtcatcgtcg gtccttcggg
     9121 atgcggcaag accaccttca tgaacacctt cgccggcctg gttcgaccgg gcaagggaaa
     9181 agtgttcatg gatggggaag aaatcaccgg tccctctgcc gagcggggag tcgtcttcca
     9241 acaattcgct cttttcccct ggaaaacggt gaaggagaac atcgaattcg ggctgaagta
     9301 ccgcgggatt cccaaagagc aacgggatga gactataagg aagtacgttc agctgatcaa
     9361 actcgaggga tttgagaagt cctatcccca cgagctgtcc ggggggatga aacagcgcgt
     9421 ggctatcgcc cgggtgtatg ccaaccggcc tcaggttctg ctcatggacg agcccttcgg
     9481 ggccttggac gctcagactc ggaccatcat gcaggaagac ctgagcgaga tgtttttgac
     9541 cgagaagcgg accgtgctct tcatcaccca cagcgtggat gaggccattt acctgggcga
     9601 ccgggtggtc atcatgaccg ccaggccggg aaagatcaaa aagatcatcg acatcaccag
     9661 ggacaacctg atcccggatc gtcgccgaat gaggtatgac gaggtggtta gtctgaagga
     9721 atttctagac ctgaaagggg aaatctgggg actgatcaag gccgagatcc tcgaaaaaca
     9781 tattctttga aggaggtggt ttcctgcggt cccttcgtgc tattttctgt ttccatcccg
     9841 aagtaggggc aattgaggaa ttgcccatgc aaagaaatct gtagaggaaa ggagaaaaga
     9901 gaatgaaagg caattggttc atgaaactgg ggatgttctc tctggccgca gcgatgattc
     9961 tggcgatgtc cacggcagct cacagccagc agaaggagct gcccctggtc cgggcgagcc
    10021 atcagccctg catgcacgcc ctgcccacca tattggcctc caccttcaac tggtgggacc
    10081 aaatcgggat ccgggtatcc tttcactatt tcgtatccgg catgccccag gtggaagccg
    10141 gacaggccgg ggaatgggac gtgggggcca tcggcacagt tccctgccta tttgccggca
    10201 tcaagtacaa acaacccctg atcgccatct ccaatgatga atccgtcacc aacaacctca
    10261 tggttcgacc cgatgccttc aacacctggc tgaaggaccc caagaaggac ctcacgggca
    10321 agaccttcct ggtctccacc atctccaccg ggcattatgc ggttgttgct tacctgaaga
    10381 agatcggcct gaaggaaagc gatgtgaaga tcgtccacat ggaacaatcc gccatcctct
    10441 ctgcctttct gtccgggcaa ggggatgtgg cccagacctg ggctcccttc aactatatga
    10501 tggaagaaaa ggggatgaaa gtcctttccc acgggaagga tgccggggta gtgattcctg
    10561 gcggagtggt agccaccaag aaattcgccg acgagaaacc ggaactggtg gccaaatggc
    10621 tggaaggcta catgcggggc attgatttca tgatcgacaa cccccgggaa tcggccaagc
    10681 atctgggaaa atacttccgg gaaaaatgcg ggatcaacct ttccgacgaa gtctgcttga
    10741 aagagtttga gcttcgcccc ctcttccgca ccaaacaaca gatcaggatg tttgaccgga
    10801 agggcgggaa gatgagcacg gtggacaagt ggatggacga tctggcccag tattttgtcg
    10861 aagtaaagaa gatcgacgcg aagcccgacc ccaagagcta catcaccgac aagttccttc
    10921 aaatggtgga caaacaggct aaaaaataat gacacagaaa cgttccgatt ggggtggggc
    10981 gcattaacgc cacacccgga attcctggaa ttcctgcgtt gacatcgaac aacctctgcc
    11041 ctatactgag ccgaacaatc cgcggccaag ggaaaaaatc tgcccggaga tggtcctacg
    11101 gagggggagg ggtattaaac ccattcttag aaaggagggt tttttatggg aaaggaagta
    11161 actcgcaggg gttttttgaa aggcatggca gcggccgggg cggttgcttt aaccgctgga
    11221 gtcgcgccga aagcctttgg tcaggcgccg gctaagggcg gtttcaactg gaagaaattt
    11281 gcgggggcca aaatccgctt catgaacaac acccatgact ggagcacaga attgatcccc
    11341 aaagctctgc cggagttcga gaagctgacg ggaatcaagg tgcagtggga ggtgctggcc
    11401 gagaaccagt tccggcaaaa gttaaccctg gagctcagct cgaacccgga ttcggtagat
    11461 gggtttatga gcctgccttc gtgggacgga atggcctttt accagtccgg gtggtacgag
    11521 ccggtggaaa aatttctcaa atcccctgat ttgaccagcc cggattttga tttcaaagat
    11581 ttcttcccga gctgcaccca aatcgccacg atcaagaaca cccttgtggg agtccccctt
    11641 tacccggaag tccagatgct gtactacccg aaagagaaat tccaggaaaa gaagctgaag
    11701 gttccggaaa ccttcgaaga gttcgcggcc accgccgaga agctctacga caagaacacc
    11761 aaatacgccg gatatgtcag ccggggcgat gggatccagg ccgtttacac cttggcccct
    11821 tttgttttcg ggatgggggg ccggtggctg gacgaaaacg gccggccgga cttgaccagc
    11881 gacgcctttg ttaaggcctt agaactttac agcggcaccc tgcacaagta cgggcctccg
    11941 ggaatcgccg ggcagacgtg ggctcagaac caggtagtct tcatgcaggg aaatgccggg
    12001 atgaacaccg actcttccaa cttcatttct acctatgagg atcccaaaag gtccaaactt
    12061 gcagggaaag tcggctacgc gcccgtcccg cgcgggccgg ccgggctgag gagttcctta
    12121 attgcctggt gtctttccat cggatcaagg tccaagaaca aagaagcaac ttggatgttc
    12181 atccagtggc tggccagcaa ggagatggtc cttcgtcagg ccaagatgaa gctgcccgtg
    12241 ggccgcgagt ctgcctggaa aagcccggag tttcagtcct cgatgtcgaa agaatggctg
    12301 gatgccttta ccaaagagct gccgacagcg gccgtcaacc aggcgaaccc cttagtcgtt
    12361 caggttccag aaacccgaga cgccatcggc aaagccatcg tctcggtcat ccagggcggc
    12421 aatgccaagg aagcggccat gaaagcgcag caggattgct tgaagatcct gaagatgacc
    12481 tgaagctgaa gtcgatcttg tccttcgaca ggcttaaggt ttcacaccgt ttcaatggtg
    12541 cgaagcggaa gcgtgagggg ccgaaggggg tataggagcc cgctggcccc tcattttttc
    12601 ttgagatgtt ttttcggtcc tgtcccggaa ggaaacccga ggacgcaaca tgagtgcgaa
    12661 tgatcccccc tctggggaga gcccgtgcat ttcctggtaa cggaagggga agccctcggg
    12721 agaccgggat atcctgtgaa atcccccctc gccccctttt taagtaggga aggacgcatc
    12781 tccccaagcg aactcgactc gaaaagggga aaatttgctc ggtcattgac gtatgaaaag
    12841 gtacaccaat cctcatatgg ttttgacaga ttccccttcc ctctggcaaa gattggtggg
    12901 tgtttgcgac cgtaacgctt cctggttgat gcccttgcca gcgatgattg tgacggtcct
    12961 gctcatggtg tttccgcttt tttatctcct gtatatgagc gtccatgact ggcttctgat
    13021 cgggggaaaa ccgccgcaat tcattggatt tcaaaattat atcgccctgt ttcaggacga
    13081 gcgctttctg gcgtctttat ggcggatcgg gatatttatc gcgctcggat tgctagtcca
    13141 gattcccgta ggtcttacga ttgctgtcct cttcaacgaa cgctttcccg ggcggggaat
    13201 catgcggacc ttgctcctgc tgcccatggt ggcaacaccg gtggccatgg ccctgatctg
    13261 ggtcatcatg atggaaccga gcagcggggt tctcaactat ttcctttcct ggtttggaat
    13321 cggagcggtg gcgtggctct cggatgtcaa tctcgtcatc ccggccctgg ttttggtgga
    13381 cacctggcaa tggacaccca tggtggctct gatctgcctt gccgggcttt ctgccctgcc
    13441 gatggaaccg ttcgaagccg cccgaatcga cggggccaat gtctggcatc gtttctggaa
    13501 gcttaccctg ccgatggtct ggcccacgct cctcatcgcc atccttttcc ggttcatcga
    13561 tcttctgaaa gtaatcgaac acatttacgt gatgacccgc ggcggtccag ggttcgcctc
    13621 ggagacgatt aacctataca atttcctcat ggggctcttt tactaccggg tcggatatgg
    13681 ttcttcgatc tcggtggtga tcttcgccat ggttcttggg gccagcctta tgctgatccg
    13741 cgcgaggagg acttcatggt aggttcaggc agggtcgccg ggtctccagt gcggcacaat
    13801 cccgcttttc gcaaagcagt ccttttggtc ttagggatat tggttttaat cccttttctc
    13861 tttccttttt tctggatgct tctcgggtcc gtaaaaacag aagtcgattt catggcctat
    13921 cccccggtct ggctcttcaa acccaccctc gagaattatg tcaaagtttt cgtcgagaat
    13981 gatttcggca ccttttccct caacagcacc atcgtagccg ctttcaccac cttcttcgcc
    14041 ctacttttcg ggattccagg ggcctatgct gtggcccggt ataagcggac aggcttcgcc
    14101 ctcattgtcc tcgccgcccg gatggcgccg gggatcgcct ttttactccc ctggttcgtt
    14161 cttttcgttc aactcaggct caccaacacg tattttgctt tggtgacctc gcacctgatt
    14221 tttactctac ccttggttct ttggatgatg attgggttca tcgaggcgat tcccaaggag
    14281 atcgagcagg cggccatggt cgatggctgc acggtttggg ggtccctgtt ccgcatcatt
    14341 gtccccttga ccctcccggg agtggccgct tcggggatcc tttgtttcat cttctcctgg
    14401 aataatttcc ttttttccgt cgtcctggcc ggagaggcaa ccaagaccct tcctgtggcc
    14461 gtctttaatt tcctgggtta tcagtccatc gcctggggcc ctctcagcgc ggccgcatcg
    14521 atcattactt tgccggtttt gattatggcc cttatcgtcc agaaatatat cgtgcaagga
    14581 ctggcagccg ggggaatcac cggctaggat tttcagtgaa aagaaggctc gttggaatca
    14641 atccatagga agctcatcaa ccccttttcg aggaggtcag atgaaaaaca ttccttacgc
    14701 tcgggtttcc catacggtca tcgaagatgt gggtcaggta aaacccgccg agaacgttct
    14761 cattctaacc gatactgagg tgagtcagac gatccccact tccttagctg aggcggttca
    14821 tgcagcaagg gggattcctt ccattctggt catggctccc cccgaatacc cgggctgccc
    14881 cctgcctgca cctgtggagt ttgccgtgca gggggcgcag gtggtgatca tggcaacctc
    14941 caaatccgtg gcccatgccg aaatattcgg gcgggagatg aagaagcgaa gactcatcag
    15001 catgcccgga atcatggaga gatctttcct cgatggagga ggaaccgcag acttgaagga
    15061 gctgaaagaa atcaccgaaa gagtatcccg agccctggag gggaaaagaa ggtttgcaat
    15121 caagagcgac aaaggaaccg atgccgccgt cttttcccgg ggcaaagctt tctccttcta
    15181 tgctcagcct cccagcccgg tgggatttgc catgtttccg gatggcgagt cggtcgtcgc
    15241 cctggaagag aagaccctgg aggggcgcat cgtcatggat gtcttccaga ccggagtggg
    15301 gcctcttcgt gagcccattg cctttgagat gaagaatggg aaggctgtcc ggattacggg
    15361 aggaattgaa gcccagctgc tgggtgaggt cctggaaaag tacggagacg ataattccta
    15421 ttacttcggg gagtttgccc tggggactaa tcccaaagca cggtttatcg gcagcgcggg
    15481 cgaagacaag aagcgcattg gaaccgtcca catgtccctg ggagatgata tagggatcgg
    15541 tggagaactg aagagcaaac tccacctgga cgccgtaatg ggtcgcccta ctctggtggc
    15601 tgacgggcag attctgatcg aagatggccg actccttgta tgattaattc ttctcttacc
    15661 ttggtgagtc gtaggggcga ttcatgaatc gcccctacga ctttaaccga tcaagtgagg
    15721 aaaacatgaa aagaaaagtc ccctataaaa agttaatcga agatttcctg ctaatctccc
    15781 accgcctgag gcagcgggaa ctgaccttcg ggggaggggg gggaatcagc attcagattc
    15841 cccgttcccg aaaagttttg atcaagggat gggaagtggc ctccgaagac gccgggaaaa
    15901 aggatatttc tctggttgat ctggatggga agcaattaaa ccaggttaaa ccatgcctgg
    15961 agacccccct gcatttagag gttttgaagg ttcgaaagga tatccgagcc gtcattcatg
    16021 ctcattctcc gtatttaact tcatttggaa atatcaaaat tagactccca aaggccgact
    16081 taaaaaatta tacgtttcta ggtgtgacca ccgctttcgc tccctacgcc gacccggggt
    16141 caaaggagct ggcagagtta gtttcgaaac cctttcaaaa ggaagaggtc ttctgcgttc
    16201 tcatggaaga tcatggggtc acggtggtgg gcacggacat tttcaatgcc tattaccgct
    16261 tggatatcct agaaggcatg gccaagacct tcatattgac gaaattgatc cgacaaatta
    16321 tttaatacaa atattggtta ttaattcaag gaattagaaa ttagtaatta ataaaatagt
    16381 tgaatttaaa tctggaggtc gggaaatgaa tgcaaaggaa atcaagaaag ttaccttgat
    16441 tggaagtggg accatggggc ccggcatcgc ccagacattt gctcgggcag gcctggaggt
    16501 cttcctggtg gacataaagc aggagattct ggataggggc atggatcgca ttggggataa
    16561 tctggacgtt tttatcgaac tggaagtgat caagcccggg gaaaaagaag ccattttaag
    16621 ccgcatccat ccgaccaccg acatggaatt tgcttgccgt caggcagatt attttatgga
    16681 agccgttcaa gaaattttgg aaagaaagca gcaggttcac cgccaggccg atcattggtg
    16741 ccgtcccgat gttattttca cgaccaatgc ctccaacatg cagatgaacc caatcgcttc
    16801 ggtcacgaaa cgcccagacc gattcgttgg gacccattgg gtcaatcctc cccacatcat
    16861 gcaactggtg gaggtggtcc gaggaccttt ggcatctgaa gcggcggtcg agacggtcta
    16921 ccaattttta aaaagagtgg gaaaagcccc ggtcatctgc aaagacactc tctcctacat
    16981 caataattac gtacaaagtg ctctgggccg ggcagctctc gagctcctgg agcaaggggt
    17041 agcgacagcg gaggatatcg atcaggcggt gaacacgggt tttgggtttc gtcttccggt
    17101 tgtagggccc ctggcttttt ttgacatggc ggggctcgat aatgtgcgag acagctggga
    17161 atatatcaat aagatgggtg gaaagatggg accgcttccg catttcattc tcgacctcat
    17221 cgctcagggc cattggggct taaagaccgg ccgcgggatc tattcctatt ccgaacaaga
    17281 aattcgggaa ctggccaagg aaaggaaccg ccagttgatt cgcatgttga aagccttgca
    17341 acggatatga ttttccgagt tccttgacac tccttcgacc ccctttctat aatgggattg
    17401 tggaagaaga aaaagcagcc tgggcatgtg ctctgcagga gcaaggattt ttccccggga
    17461 gctgcaccgt ttcaacggcg ggtaggaagg ccccggcttg tcaggccact ttgcggaaaa
    17521 agaatgacca tttcgagaga ttgatctcgg ccgtccatct ctcccgtccg gaaaattacc
    17581 tttccatata ccagtcgggc tgcaacttct cctgccgcaa gtgccattca tggtatttca
    17641 gcaaaaaggc cgaaggaaag tggcataccc ccaaggaaat actccaaaag tgcatcgaat
    17701 acgatcaaaa ggtcaatttg aatgagccgc ggttcaaggc caccgcttat catgcccacg
    17761 atacctgccg ctgttgcggc tcctgcgtcc ttcacggaaa gcagtcttcc ctttgccccg
    17821 gaattctgga gccctccgca atcgtcctca gcccccaagg attcggaccg gtccgcaata
    17881 tcgtcggctt caccggtggg gacctcacct gtcaaccgaa gttctatgcc cgttgctctg
    17941 agctgatcaa atcccatacc cgtctctggg ttttgatcga gaccaacgga tatggtctca
    18001 cccccaatca tctagacctg ctgcggagcg cgggggtgga tgcctactgg ctggatatca
    18061 aggcctttcg ggaagatacc catcactggc ttacagggtg caccaatgaa tggattttga
    18121 aactgccgga ggaaatgaag aaaagggatt ttaccgtgga agttctttcc ctctacatcc
    18181 cccaagtggt ggagacggac caactggaag ccattgcagc tcacctggcc cgaatcgacc
    18241 gggatattcc tttcaccatt ctggctttct ttccggagta ccagatgaag gaatatcgcc
    18301 ccccgacgct cgaagaaatg gttggcgctt ttcaggcggt cgaaggcgcg ggccttaaaa
    18361 atgtgaggct gggaaatacg ggaatctttg ctcataccga agatcaaatt caaaccctac
    18421 ggcaaaaagt cagaatgggt catttttaag ggtgggagat gctgggaata tcgagcgctt
    18481 ggcgatcgga aattttggac agcgggagag agatcgtaga ggagatattg aacctggggg
    18541 tgcaggcgat ggaactggag taccggatga cccgttccat gctgaaagag gttgtccccc
    18601 tggtcaaagc aggg
//
LOCUS       DASPRR010000268         7890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1508427, whole genome shotgun sequence.
ACCESSION   DASPRR010000268 DASPRR010000000
VERSION     DASPRR010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508427"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VEL68_10500"
     CDS             <1..690
                     /locus_tag="VEL68_10500"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36438.1"
                     /translation="NKEFATILGPSGCGKSTLLRIVAGLTKPTSGVARMDGKVITEPG
                     QDRGMVFQSYTLFPWLTVKENIQFGLELSGLDKDRQEQIARDFVEKVGLRGFENTFPK
                     GLSGGMKQRVAIARALANNPAILLLDEPFGALDAQTRSLMQELLTQVWEELHKTILFV
                     THDVEEAIFLSDRVFVMTARPGKIKAEIGIPLPRPRSYEIKATEDFLALKGLALTLIR
                     EEAIKAAHIEP"
     gene            complement(726..1643)
                     /locus_tag="VEL68_10505"
     CDS             complement(726..1643)
                     /locus_tag="VEL68_10505"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXZ36439.1"
                     /translation="MKAAVLHGLRDIQIAEVPEPKPSPGEVLVRVKYCGICGSDLHEY
                     QHGLFPESPFGHEACGEVVELGAQVKGFQVGDRVLSFFKGAYAQYMVCPQERLLGIPP
                     NMTWERAAVVEPLAVAAYSVEKANIQPGITALIAGAGPVGLMTILGLRCLGVNQVYVT
                     EVSDPRREKAKELGATLTCNPLETKVSSWIKEITSGRGVDVAFESVGIEATLKDCLTS
                     VRFQGKVVVQGIFTDRVPIHMLGFVSRETTMIGVNSANPPLALKWIETKGIQPERMVT
                     RVLPLGRISEAFGLLTQQTKQEIKILVEP"
     gene            2143..2376
                     /locus_tag="VEL68_10510"
     CDS             2143..2376
                     /locus_tag="VEL68_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ36440.1"
                     /translation="MEIKLFANFREYLPPGSDKYSCWVDLEEGSTIGQALEQLKIPVS
                     IPMITLVNGLHRSFEDILHPGDILSIFPPVAGG"
     gene            2499..3266
                     /gene="tolQ"
                     /locus_tag="VEL68_10515"
     CDS             2499..3266
                     /gene="tolQ"
                     /locus_tag="VEL68_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HXZ36441.1"
                     /translation="MNTITLWSLGQLFPSGTVGGFGGGIVDLILYSGPVVKAVLLILL
                     FFSIFSWAIIFAKLRLIRLADSESRSFLRIFWEGKQFASIFAESKKLRHSPTAEIFRA
                     AYMELTKLSQAKSNPDSSRKGSDPTSFSMELSGVENLNRAMRQALTSELTKLERALGF
                     LATTGSTTPFIGLFGTVWGIMDAFRGIGARGSATLAVVAPGISEALIATAAGLAAAIP
                     AVVAYNYYINRIKVLSAQMENFSSELLNIIERHFLKA"
     gene            3522..3935
                     /gene="tolR"
                     /locus_tag="VEL68_10520"
     CDS             3522..3935
                     /gene="tolR"
                     /locus_tag="VEL68_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HXZ36442.1"
                     /translation="MQGPKAENRPMADINVTPLVDVILVLLIIFMVTAPMMQQGVDVE
                     LPQTTSQAMEGQEERLVITINAKREVYLNQEKVDAALLRRHLERSTAARLNREVFLRA
                     DRSVPYGFVVRIMAEIKNAGIEKLGMVTEPLETKK"
     gene            3932..4807
                     /locus_tag="VEL68_10525"
     CDS             3932..4807
                     /locus_tag="VEL68_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXZ36443.1"
                     /translation="MKVSPAIQNLVPIPHDPRMGGMFLLSLFLHLMAVSAVFFIPNLS
                     TQRTYYSPVYSVRLVNVQPAPPPAVRGAESKKETQPPVSAAPVEKAKPKEKEKPVSLA
                     TKPKEDSTKVAEAIERIRQRREEKKVDTAIDRLRQRQEEKNIDSAIDRIRGDREAKQI
                     DSAIEQIRKKVTIGSAGAIETGESGTGGASSGFMSIKHKMYYNLIWQRIRSAWVLPEG
                     ALGGKKDLETIIAIRIAKDGRIEDIQFEKKSGNPQLDESALRAIKKANPLPPLPPGFE
                     EDKFDVGVRFTPSDV"
     gene            4817..6184
                     /gene="tolB"
                     /locus_tag="VEL68_10530"
     CDS             4817..6184
                     /gene="tolB"
                     /locus_tag="VEL68_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXZ36444.1"
                     /translation="MKKPVLFLPAVLLLLLILAHHLSAKVYIDIRSPSFRKFPLAIST
                     FQSSGPTNREMQLGERATEILKNDLDISGFFTLITPSEVTEKNAPPGGSDSSAKPGED
                     RPGAGGEPPNFQPWANLGAEALVVGNLSYRDGSLTLEARLHDVVKKEMIVGKRYLGEI
                     TDLSRMVHRFADEIIFALTGEQGFFQTKIIFVSQVGGFKELYMMDYDGRNVFPITNHR
                     SICLSPRVSPDGRWVAFTSYKLGNPDLFLKDLQTGEERRISSYPGLNISPAWSPDGKR
                     IALTLSKDGNPEIYTMNPDGSGLTRLTNHPAIDVSPTWSPDGQRIAFVSSRGGNPQIY
                     VMQADGQNVQRLTFEGNYNTSPAWSPRGERIAFDATMGGGRNICLMNSDGSDLRVLTA
                     NMGQCEMPSWSPDGRQLVFSATRDGDPRIFLMNAEGTRIKRLTFQKGSDTYPYWVPRN
                     REIPK"
     gene            6225..6812
                     /locus_tag="VEL68_10535"
     CDS             6225..6812
                     /locus_tag="VEL68_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXZ36445.1"
                     /translation="MLRKASWLLVIVVSIVAVSLLAAGCSKKAVVKEEVAARPAVEAK
                     VEPPPPPPPPPPPPPPPPPPLPPPPPKVEEAPPKPAVEPIDLVGLRLQFAFDDYSLSS
                     KAKENLEKLATWMKKAEEAKVQIEGHACEIGTNEYNLALGERRANSAKTYLEGLGITG
                     GRLSTISYGEERPLDPGHTEEARSKNRRAEFVMTK"
     gene            6897..>7890
                     /gene="ybgF"
                     /locus_tag="VEL68_10540"
     CDS             6897..>7890
                     /gene="ybgF"
                     /locus_tag="VEL68_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXZ36446.1"
                     /translation="MKLTWNQILLGFLLFGIWGFSGCATQGDLDAVQRDSNSLTKEFL
                     GLQRNLYDMNAELKDLSAKLDSLDKRTGNLQRDVGALSGEMKTKMGLMEKEMESSSQP
                     MRRYQADLGARVDKLQLEVQNLTGRFEESKYFAEKTFGATKNYQARLEETEKRLAALT
                     KPSESVEKKPEASVKPSGEEEKPSAVSPTPEATKETPVTKPEVVKKPVAGPDEAYKKA
                     YELYSKGKIDDAQKEFKKFLEAYPKSKYTENSHFWLGECYFSQKKYEDAILEFDEVIK
                     KFPKGNKVADALYRQGMAFLEMKDTTNAKLILKEVVRRFPQSDQANRARKKLQEI"
BASE COUNT         1860 a         2187 c         2195 g         1648 t
ORIGIN      
        1 aataaggaat tcgccaccat cctgggccct tcgggatgcg ggaaatccac ccttctccgg
       61 atcgtggccg gattgaccaa gcccacctca ggggtagccc ggatggatgg caaggtcatc
      121 accgagcccg gccaggaccg aggcatggta ttccagagct acacattatt cccctggctg
      181 acggtgaagg agaatattca attcgggctg gagctatcgg gcctggataa ggacaggcag
      241 gaacagatcg cccgggattt tgtagaaaag gtcggtctga gagggtttga gaataccttt
      301 cccaagggac tctccggagg gatgaagcag agggtggcca tcgcccgggc gctggcgaac
      361 aacccggcga tcctgctgtt ggatgagcct tttggggccc tggatgctca gactcgctcc
      421 ctcatgcagg aactcctgac ccaggtatgg gaagaactgc ataagacgat tctatttgtg
      481 acccatgatg tggaggaggc catcttcctg tccgaccggg tcttcgtcat gaccgcccgg
      541 ccgggaaaaa tcaaagccga aatcggtatc ccccttcccc ggcccagaag ctacgaaatc
      601 aaggccaccg aagactttct ggccctgaaa ggactagctc taaccctcat ccgcgaagag
      661 gccatcaagg ccgcccacat cgaaccctga aaatcgatca tcaggaagag ccatggcttt
      721 gcggatcagg gctcaaccag gattttgatt tcctgcttgg tttgctgggt gagaagccca
      781 aaagcctcgg agattcttcc aagaggaagg acccgggtga ccatgcgttc gggctggatc
      841 cctttggttt caatccattt caaggccagg ggagggttgg cggagttgac cccgatcatg
      901 gtggtttccc gggatacgaa tccgagcatg tggatgggaa cccggtcggt gaagattccc
      961 tggacgacga cctttccctg aaaccgcacc gaggtaaggc agtctttcag agttgcctca
     1021 attcccacgg attcaaaggc cacgtccacg ccgcggccgg aagtgatttc cttgatccag
     1081 gatgacactt tggtctccag cgggttacag gtcaaggtcg ccccgagttc ttttgccttc
     1141 tccctcctgg ggtctgaaac ttcggtcaca tagacctgat tcactccgag gcagcggagg
     1201 cccaggatag tcatgaggcc caccggtccg gccccagcga tcaaggccgt aattcctggt
     1261 tgaatatttg ctttctcgac ggaataggca gccaccgcca ggggttcgac aacggccgcc
     1321 ctttcccaag tcatattcgg aggaatccca agcaagcgct cctgggggca taccatatac
     1381 tgagcatagg ctcccttgaa gaaagagagg actcggtctc caacctgaaa tcctttgacc
     1441 tgggcgccca attccacgac ttctccgcag gcctcatgtc cgaaggggct ctccggaaaa
     1501 agcccgtgct ggtattcgtg taaatccgac ccgcagattc cacagtattt cactctgact
     1561 aaaacctcgc cagggctcgg tttgggctcg ggcacctctg cgatctgaat gtcccgtaag
     1621 ccatgaagaa ccgccgcttt caaaatgtcc tgattccttt tcgaggtttg atattcaagc
     1681 agatgtattg ggagattaac aaaatagggg agagggtgtc aacggggtta ccgagcttgc
     1741 ccacccccag tttttcgtgc ttgttggggt tcagggtgga ggaatttgga tcatcaggct
     1801 cggcgggtgc ttacccttcc atgaaactcc tccccatgtt ccgcaggcag aggcctgaga
     1861 ggaaattcct ccaagcagaa aagagaacca aagcattatt ccgtctcaat ccccccagga
     1921 caggcccgat ccaatggaag aaccaccgcc tcaggatgat gatccaaatt tctccggcct
     1981 gaaccccatg agatccccct cctttccaga gatcttattt tcgaacgact cttttttaag
     2041 aactgggggc gggcaaggct gcgataagaa gattgacttt tcgaggaaat tgttcaatat
     2101 ttatccaagg tagatcgaat tataggtttc attcagatga ggatggaaat caaactattc
     2161 gccaattttc gcgagtatct tccccccgga agcgataaat actcctgctg ggtggacttg
     2221 gaagagggga gtaccatcgg tcaggcgctg gagcaactca agattcccgt ttccatcccc
     2281 atgattactt tggtcaacgg gttgcatcgt tcctttgaag acatcctcca tcccggggat
     2341 attctcagca tctttcctcc cgtggcggga gggtgaaaag gattctgggt ttcagatccc
     2401 ggatcgaaat cgcttcgctt tcttcggaac gatataggcg ctttctcttc gggaatggta
     2461 agcacgcagg ctgaaattct ggcggcaaag gagaatcctt gaataccata acgctttgga
     2521 gtctggggca gctctttccc tctgggacgg tcggcggatt tggagggggc attgtggatt
     2581 tgatcctcta ctctggccca gtggtgaaag ccgtcctcct cattctccta ttcttttcca
     2641 tcttttcctg ggccatcata ttcgccaaat tgcgcctcat ccgcctggca gacagtgaat
     2701 caaggtcctt tctgcggatt ttctgggagg gaaagcagtt cgcttccatc tttgccgaat
     2761 ccaaaaagct ccgccatagt cccacggcgg agatcttccg tgccgcctac atggagttga
     2821 ctaaactcag ccaggcgaag agcaatccgg acagctcccg gaaaggcagc gacccgacct
     2881 ccttcagcat ggagctgagc ggggtggaaa acctcaaccg ggccatgcgc caggccttga
     2941 cttccgagtt gaccaaattg gaacgggctc tggggtttct ggccaccacc gggtccacaa
     3001 cccccttcat cggcctcttc ggcacggtgt gggggatcat ggatgccttc cggggaatcg
     3061 gcgcccgggg gtcagcgacc ctggcggtgg tggctccggg aatttccgaa gctctgattg
     3121 ccaccgcagc cggattggct gcggctattc cggcggtggt cgcctataat tactacatca
     3181 atcgcatcaa agtgctcagc gcgcagatgg aaaacttctc ttccgagctg ctgaacatca
     3241 ttgaaagaca ctttctaaaa gcctgatttg gaactggctt ggggaggcgg caccgaaggc
     3301 tgctctcctc caagcaagag gttgagatcg aaatccgaaa caaattcgaa tttcagattg
     3361 tccaaggagt aaaaaggagg aaaacctcca aaggatgggt ttactttttt gactccttga
     3421 gggtttgcgg ggtgaaattt tgacttacct gactgaggat ttctcggaag ggtaaaggag
     3481 tcccgccccg gtgtcgatgg atttggagag agtaggttct tatgcagggt cccaaagctg
     3541 aaaatcgtcc catggcggac atcaacgtga cccccctggt cgacgtgatc ctggtccttc
     3601 tgatcatctt catggtaacc gcgccgatga tgcagcaggg ggtcgatgtg gaactgcccc
     3661 agaccacgtc tcaggccatg gagggccaag aggagcggct ggtgatcacg atcaacgcca
     3721 aacgcgaagt ctacctcaat caggagaaag tggacgccgc tctcctgcgc cggcacctgg
     3781 aacgatccac cgcggcccga ctgaaccggg aggttttcct ccgggcggac cggagcgttc
     3841 cctacggttt cgtggtccgg atcatggccg agatcaaaaa tgccggaatc gaaaaactgg
     3901 gcatggtcac cgagcctctg gaaacgaaga aatgaaggtt tcccccgcca ttcaaaacct
     3961 ggtccccatt cctcatgatc cgaggatggg gggaatgttt cttctctcct tgttcctcca
     4021 tctgatggct gtttccgcgg tgttcttcat ccctaacctc tctacgcagc gaacctacta
     4081 ttcccccgtc tactccgtac gactggtgaa cgtgcagccc gcgccacccc ctgccgtacg
     4141 aggcgcagag tcgaaaaagg aaacccagcc cccggtctcc gctgcgccgg tggaaaaggc
     4201 gaagcccaaa gagaaagaaa agcccgtctc tctggcgacc aagccgaagg aggattcaac
     4261 gaaggtcgcg gaagcgatcg agcggatccg gcaacggcgg gaggaaaaga aagtggatac
     4321 ggccatcgac cgccttcggc aacggcagga agagaagaac atcgactcgg ccattgaccg
     4381 catccgcggg gatcgggaag ccaagcagat cgattcggcc atcgagcaga ttcggaaaaa
     4441 ggtgactatc ggaagcgccg gggccattga aacgggcgaa tccggaacgg gcggggcctc
     4501 ttcgggattc atgtccatca agcataaaat gtattacaac ctgatctggc agcggatccg
     4561 cagcgcctgg gtgcttccgg agggggccct gggagggaaa aaagacctgg aaaccatcat
     4621 cgccatccgg atcgccaagg acgggcggat cgaagatatc cagttcgaga agaaatccgg
     4681 aaatccccaa ctggacgaat cggcgctgcg ggccatcaag aaagccaacc cccttcctcc
     4741 cctacctccc gggttcgaag aggacaaatt cgatgtggga gtccgtttta ctccctctga
     4801 cgtttgaggc cgatccatga aaaaaccagt tctttttttg cctgcggttt tgcttttgct
     4861 cctcatcctg gcccatcacc tttcggccaa ggtctatatc gatatccggt ctccttcttt
     4921 ccggaagttt cccctcgcca tctccacctt tcaatcttcg ggcccgacga accgggaaat
     4981 gcaattggga gaaagggcta cggagatttt aaagaacgat ctggatatct ccgggttctt
     5041 taccctcatc accccgtcag aagttactga aaagaatgcc ccccctgggg gaagcgattc
     5101 ctcggccaag ccgggggagg atcgtccggg agcgggcggg gagcctccga actttcagcc
     5161 ctgggccaac ctgggggcgg aagccctggt ggtggggaac ctttcctatc gggacgggtc
     5221 cctcaccctg gaagcccgcc ttcacgatgt ggtgaagaaa gaaatgatcg tgggcaagcg
     5281 ttacctcggg gagatcaccg atttatcacg catggtccat cgctttgccg acgaaatcat
     5341 cttcgccctc accggggagc aagggttctt ccagaccaaa atcatctttg tctcccaagt
     5401 gggagggttt aaagagctct acatgatgga ttacgatggg aggaacgttt ttccgatcac
     5461 caaccatagg tccatctgcc tgtctccgcg ggtttcccca gacggaaggt gggtggcctt
     5521 cacctcttac aagcttggga acccggacct tttcttgaaa gacctgcaaa ccggggagga
     5581 aagaaggatt tccagctatc ccggcctcaa catctctccg gcttggtccc ccgatgggaa
     5641 acggatcgct ttgaccctga gcaaggatgg aaacccagaa atctatacca tgaacccgga
     5701 tggttcggga ttgacccggc tgaccaatca tccggccatc gatgtctctc ccacctggtc
     5761 ccccgacggc cagaggattg cctttgtctc cagccgggga gggaatccgc agatttacgt
     5821 catgcaggcc gatggccaga atgtccagcg gctgaccttc gaaggaaatt acaacacctc
     5881 cccggcctgg tctccccggg gagaacggat tgccttcgac gccaccatgg gaggagggcg
     5941 aaacatctgc ctcatgaatt ccgacgggtc cgatttgagg gttttgaccg ctaacatggg
     6001 acagtgcgag atgccgtcct ggtcgcccga cggacgccag ctggttttca gcgccacccg
     6061 ggatggagac cctcggattt ttttgatgaa tgccgaagga acccgaataa aaaggttgac
     6121 atttcaaaag ggaagtgata catatccata ctgggttccc cgaaatcgag agattcccaa
     6181 gtagttttgt gggaattcga agcataaaaa gaaggaggta agagatgctg agaaaagctt
     6241 catggctgtt ggttattgtt gtttccatcg ttgccgtgag tctcctggcc gccggttgct
     6301 ccaaaaaggc cgtggtgaag gaagaagtgg cggcccgtcc ggcagtagag gcgaaggttg
     6361 aacctcctcc cccgcctccg ccgcccccgc ctccgccgcc tccgccgcct ccgcccctgc
     6421 ctcctcctcc tcccaaggta gaggaagccc ctcccaaacc ggccgtggaa cccattgatc
     6481 tggtgggcct ccgccttcag tttgccttcg acgactattc cctctccagc aaagccaaag
     6541 agaacctgga aaaactggcc acatggatga agaaggcaga agaggccaag gtccagattg
     6601 aaggccatgc ctgcgagatc gggaccaatg aatacaacct ggccctgggc gagcggcgcg
     6661 ctaacagcgc caaaacctac ttggaaggcc tgggaatcac tgggggaaga ctctccacca
     6721 tcagctacgg ggaagagcgt cccctggacc cggggcatac ggaagaggcc cgctccaaga
     6781 accgccgggc tgaattcgtg atgactaaat aatggatctc tactttcagg gagctctttt
     6841 catgggtcgg ggtgttcagc ccgcgggaag agctccctgg gctttgagat gatccgatga
     6901 aattgacctg gaatcaaatc cttctggggt tccttctgtt cggaatttgg ggcttcagcg
     6961 gctgcgccac tcagggcgat ctggatgccg tccagcggga ttccaacagc ttgaccaagg
     7021 aatttctggg gctccagcgg aacctttatg atatgaatgc ggagttgaag gatctctcgg
     7081 ccaagctgga cagcctagat aaacgcaccg gaaatctcca gcgggacgtg ggggccttaa
     7141 gcggcgaaat gaaaaccaaa atgggcctga tggagaaaga gatggaaagc agcagccagc
     7201 ccatgcgccg ctaccaggcc gatttggggg cccgggtgga caagcttcag ctggaggtcc
     7261 agaacctaac cgggcgcttc gaggagagca aatactttgc cgagaaaacc ttcggggcta
     7321 ccaaaaacta ccaggccagg ctggaagaaa cggaaaagcg gcttgccgcc ctgaccaaac
     7381 cctcggagag cgtggaaaaa aagccggaag cctcggtaaa gccgtctggg gaagaagaaa
     7441 aaccgtcggc cgtcagccct acgcccgagg cgaccaaaga gactcccgtt acgaagcctg
     7501 aagttgtcaa aaaacctgtg gccggcccgg acgaggccta taagaaagcc tacgagctct
     7561 acagcaaggg aaagatcgac gatgcacaga aggagttcaa aaaattcctg gaggcctatc
     7621 ccaaatccaa gtatactgag aattcccact tttggctggg ggagtgctat ttctcccaga
     7681 agaagtatga agacgcgatt ttagagttcg atgaagtcat taaaaaattt cccaagggaa
     7741 ataaggttgc cgacgccctc taccgccagg gaatggcttt tctggagatg aaagatacga
     7801 ccaacgccaa attgatcctg aaagaggtcg tccgccgctt cccccagtcc gaccaggcca
     7861 accgggcccg caagaaactt caagaaatcg
//
LOCUS       DASPRR010000269        10230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2155910, whole genome shotgun sequence.
ACCESSION   DASPRR010000269 DASPRR010000000
VERSION     DASPRR010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155910"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VEL68_10545"
     CDS             complement(<1..593)
                     /locus_tag="VEL68_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36447.1"
                     /translation="MRIQITLTSPDGKRIIAKGIRALPVVQKVQNRGKILLKGGTTVS
                     AVSEELCGKPMRISGMITPKGTLTSRFKHEIQWAHAQILKGGRIIPLHTREDWEKEIP
                     SFTPEDLVITGANAFDAHGQATLMAATYDGGSSLPFFQTLLIEGVPFLIAAGLEKLVP
                     GNLGEVIPLAGRKKVSFSYGSAVGLIPIFGRIFTEVEA"
     gene            862..1863
                     /locus_tag="VEL68_10550"
     CDS             862..1863
                     /locus_tag="VEL68_10550"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HXZ36448.1"
                     /translation="MKKDIRVLQFGIGVIGRGVTKVLARKRGMKIVGAIDVTNVGKDL
                     GEVAGVGRKLGVKISNDFNKVVKQWKPQVAIHTTSSSLRKVYPELERLIKARVKVVSS
                     CEELSYPYQKAPALAARLDRLAKKSGVTVLGTGVNPGFLMDAWPLFMSGVCTDVKEVK
                     VSRVQDASSRRIPFQEKIGAGKTKKEFQKLVQKGTIRHVGLPESVAMIAAGLGWKVDR
                     VTETIGPILYRKTVKSDYITVKPGRVAGVKQVGRGWSKGKPLITLHFEASIGSGESYD
                     SVSIKGTPDMEVIVKGGTHGDIATVAMLTNAVSRIFDIPPGLKTMKDLIISTVPNKL"
     gene            1929..2390
                     /locus_tag="VEL68_10555"
     CDS             1929..2390
                     /locus_tag="VEL68_10555"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ36449.1"
                     /translation="MNREKITGIFLLGLGLAVFLKSLTYPLGTLRRPGGGLFPLIASA
                     FLMGLSALLTLQAFRGKEVDKSSQSPFFSEKEAPRRILLGFAGLLAYRYLLPVIGFAV
                     STAVFIFFLSRFLGRLSWGMSVVFAIITAVVSYYLFQVWLKIPLPMPYFGV"
     gene            2394..3905
                     /locus_tag="VEL68_10560"
     CDS             2394..3905
                     /locus_tag="VEL68_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ36450.1"
                     /translation="MEVFQNMVTGFAIAITPMNLVLCLVGCFVGTLIGVLPGIGPAAT
                     IALLLPVTFNLPPATAIIMLSGIFYGAMYGGSTTAILVNIPGEAASAITCLDGYQMAR
                     QGRAGPALGIAAFGSFIAGTFGVFLLMLIAPPVADMALKFGPPEIFAVMVLGLTILNY
                     LSSGSLIKAFIIGLIGLFIGMIGIDIAGIYRFTFGIPDFYDGIGLVPVLMGLFGVAEV
                     LLNLEQIVTRHIFETRIKGLLPNREDWKRSAMPILRGSFIGFVMGVIPGPSNTISSFV
                     SYAMEKRLSKSPDRFGKGAIEGVAGPESANNSACAGNFIPVLTLGIPTSAVMAVIVGA
                     FIIHGVTPGPLLIKNNPDIFWGTIASMYIGNIMLLILNLPLIGIWVRVLKVPYGILFP
                     LILLFCFVGAYANNNSLAELNVMIFFGLVGYLMKKTGFEPAPLVMAYILCPFLEEAFR
                     QSLIKSHGDFSIFIKRPISATILAIAAGIIVLNVFSGVKQRRKSLLEKIKEED"
     gene            3986..4966
                     /locus_tag="VEL68_10565"
     CDS             3986..4966
                     /locus_tag="VEL68_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36451.1"
                     /translation="MKTRKQHMMMVAVWVTLSLLLTANGFAQEKFPSRPIQFIIPWAP
                     GGGGTVNAQALQPHFEKAIGGSVQIINKPGGGGTIAWNYVANAAPDGYTVGVTNQSFI
                     LTQYTTRTGVSFKKVDPIIMVIDMPAALAVKTDAPWKNFKEYIGYAKANPGKVQMGNS
                     GMGANYHVSALGIEMVTGVKLTHVPFKGAGPSITALLGGHVDSAVSELPTLLPYVEGK
                     KFRLLVVGSPERNPAVPDIPTMKEEGYEQILIGAWYVWSAPKGVPKNRFQKLHDAFKA
                     AMEAPEYRELYRKLGGVARYMGPQELPAFYEQEDRALKKIIDFSGFKPIE"
     gene            5036..6007
                     /locus_tag="VEL68_10570"
     CDS             5036..6007
                     /locus_tag="VEL68_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36452.1"
                     /translation="MNQKNIRVIIALTGVLSVIFAGPVWAQEKFPSRPIQFIIPWAAG
                     GGGTINAQSLQPHFEKAIGGSVQIVNKPGGAGTIAWNFVANSPPDGYTTGIVNPSTVV
                     TLYTTKTGVPLDRFDPIAYTVYIPAGVVVRTESPWKTFREFIDHAKANPGKVQMANSG
                     HAAMYHIGIVGIEMAMGVKFTHVPFKGTGPCITALMGGHVDGSLNEISTLLPYVESKK
                     FRILAVCSPNRSPAVPDAPTFKEFGFDLDVGTWYAYVAAKGTPQDRLKKLQDAFRAGV
                     ESNEFKSLYQKQGGMAMFKGPEALVAYFKEQDRLWKKIIEFGEFKPE"
     gene            6159..7172
                     /locus_tag="VEL68_10575"
     CDS             6159..7172
                     /locus_tag="VEL68_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ36453.1"
                     /translation="MRFFSRLLILFGLCFLAAAGSVPSAGANGRVVVYSSNQQAQNDM
                     MAEAFEKATGVKCEMVRAGTGVLVKKMIAEKNRPLGDVALGLGKSVLINNLDLWEPYK
                     VKDFSAYPSEYKDANGMWIGKIVHLMVFMYHTKLLTPAQAPKAWADFLDPRWQNKVAF
                     CNPSNAASSYTQLTTMLSLWGDDQAGWKKVETFLKHALITEQSANVYTNVANGEIPAG
                     ITMEYAAYRYKKGGGPIEIVNPAEGTVAYAEGLAIIKGAANRENARKFMDWATGLEAR
                     KLVLSQFLRRPAREDMDFGNLAPGMVPMSKIKLLKSFDEDYWIKKQPEVLQKVKDILL
                     RVK"
     gene            7382..8158
                     /locus_tag="VEL68_10580"
     CDS             7382..8158
                     /locus_tag="VEL68_10580"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HXZ36454.1"
                     /translation="MKIDISADVGESFGRWKLGYDEELMEYISSANLACGFHAGDPMV
                     MRNTVKLCKKYGVKVGTHVGFPDLMGFGRRLMALTPEEYLNYSLYQGGALQAIARAEG
                     EELQHFTWHGSAGTSFNADNEEIARAGIAAVAQLDRNIIVPLIYGPKGDLMAREAGKA
                     GLKVVRKFFADRALEANGMLVSRKKAGSVITDAEECAARTLRMVQDHKVKTINGKDID
                     CFGQTIMVHGDTPTAVKVAQTIRNRLEGAGIQIVPMKELI"
     gene            8439..9392
                     /locus_tag="VEL68_10585"
     CDS             8439..9392
                     /locus_tag="VEL68_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HXZ36455.1"
                     /translation="MSAFEVIQPGAFTTVQDLGRYGYQKYGVSISGAMDRFALRVANL
                     LVGNDEGEGVIEATIIGPKLKALERMRVAITGADLSPEFGGKPLPLWRTLDIPEGGTL
                     SFGAPRSGCRAYLAVAGGIDFPVVMGSRSIHTRSNLGGNGRTLVKGDIIERKDSGFRI
                     QESETHRLLPELVPVYGKNWQIRVIFGPQKDYFTRQGIETFLTSEYEITPQADRMGYR
                     LKGPKIEHKSGADILTDATPPGSIQVPGDGMPIILLADGQTTGGYSKIATVISVDQDL
                     LAQARPGEKVRFQKVTILEAHQFLREMEEKIQRIKKALIPV"
     gene            9389..10120
                     /locus_tag="VEL68_10590"
     CDS             9389..10120
                     /locus_tag="VEL68_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HXZ36456.1"
                     /translation="MSGYPKARYLLVGDHGISVEFGNSLEEEINLKVQKLMHALEKAK
                     IPGVIETNPTIRSLFVYYDPLRIGVRKLIKEMRKMEGRMADIFLPKSRLFEVPIVYGG
                     KYGPDLEEVAKLLNLTPEEIIRIHSGKEYFIYDTGFIGGSAHFKVPPPLDLLPRKKTP
                     NLGVPAGAVLIAGGLGSVYKPLAGPTGWYWIGMSPLKQWFPQKDPPLLIHPGDKVIYR
                     PVDEEEFDRIKKRVEEGNFTAKILS"
BASE COUNT         2410 a         2635 c         2928 g         2257 t
ORIGIN      
        1 gcttccactt cggtaaagat gcggccgaaa atgggaatca aacccacggc agagccgtag
       61 gaaaagctaa ccttcttcct gcctgccaga ggaatcactt ccccgagatt tccaggaact
      121 aatttttcaa ggccggcggc gatcaggaaa gggaccccct cgatgagcag ggtttgaaag
      181 aagggcaacg agcttcctcc atcataggtg gccgccatga gcgttgcctg gccatgagca
      241 tcgaaggcat tggctccggt gatgacgagg tcttctggag taaaagaagg gatctctttt
      301 tcccaatcct cccgggtgtg gaggggaatg attcggcccc ccttcagaat ttgggcatgg
      361 gcccattgaa tttcatgctt gaatcgggaa gtcaaggtcc ctttgggagt gatcatcccc
      421 gagatcctca tcggctttcc acagagttct tcggataccg ccgaaaccgt agtcccccct
      481 ttcaatagga ttttcccccg attctgaact ttttggacca ctggaagagc cctgattccc
      541 ttggctatga tcctttttcc atcaggagaa gtcagagtaa tttgaatccg cataaaattc
      601 tactcctcta agcgatcttg ttcgatcata gaaaaatccc ggagggaaag caagggagag
      661 acaaagagca ggtgtgtgct gtggtccaca aatttaagaa aacagaaaag ggagagagcc
      721 atcggaaaca tcatgtggat gaattccttg caccgggccg ggaaaaattt tctttccgag
      781 ttcatttcag ggtgcttaca tggtaatata ctggctgccc aggaaggcgc attcaaaata
      841 tctattccct cggaggcaga gatgaaaaaa gatattcgtg tgttgcagtt tgggatcggg
      901 gtcattgggc ggggagtgac caaggtattg gccaggaaac ggggcatgaa gatcgtggga
      961 gccattgacg tgaccaacgt gggcaaggac ctgggggaag tggccggggt cgggcggaag
     1021 ctgggggtca agatatcaaa cgatttcaat aaagtcgtga agcagtggaa accgcaagtg
     1081 gccatccaca ccaccagctc gagcctcagg aaggtctacc cggagctgga gaggctgatc
     1141 aaggcgaggg taaaggtggt gtccagctgc gaagagcttt cctatccgta ccagaaagcc
     1201 cctgcacttg cggcccgctt ggaccggctg gccaagaaat ccggggtaac ggtcctggga
     1261 accggagtaa atccgggttt cctgatggat gcctggcccc tgttcatgtc gggcgtatgc
     1321 accgacgtaa aggaggtcaa ggtcagccgg gttcaggatg cctcctcccg ccgaattccc
     1381 ttccaggaga agattggtgc ggggaagacg aaaaaagaat ttcagaagct ggtccagaag
     1441 ggaactatcc gccatgtagg gcttcccgag tccgtggcca tgattgcggc cggcctggga
     1501 tggaaggtgg acagagttac cgagaccatc ggaccgattc tataccggaa aaccgtgaag
     1561 agtgactaca ttaccgtgaa acccggccgc gtggccgggg tcaagcaggt agggaggggg
     1621 tggagcaagg ggaagccatt aattaccctc cactttgagg cttccatcgg atccggtgag
     1681 tcttacgatt ctgtttcgat caaagggact ccggatatgg aggtcatcgt caagggaggt
     1741 acacacgggg atatcgccac cgtggccatg ctgactaacg cggtctccag aattttcgac
     1801 attcctccgg gactgaagac catgaaggac ctgatcatct ctacagtccc caataaactc
     1861 tagagcaaag ggcattaaga aatgggtgtg ggaccggatg aaaggaagca tggcagggcg
     1921 ccgaaaagat gaatcgggag aagatcaccg gaatttttct cttagggttg gggctggcgg
     1981 ttttcctgaa gtcgctcacc taccccctgg ggactcttcg gaggccgggg ggtggactct
     2041 ttcccctgat cgcttccgct ttcctcatgg gactttccgc cctgctaact cttcaggctt
     2101 ttcggggtaa agaggttgac aaatcttctc agtctccctt tttctcggaa aaggaagctc
     2161 cgcgaaggat cctcctggga tttgccggat tgctggctta tcggtatctc ctcccggtca
     2221 tcggttttgc cgtatctacg gccgtcttca ttttcttcct gagccggttt ctgggaagat
     2281 taagttgggg catgagcgtt gtttttgcaa ttatcaccgc cgtcgtatcc tattatttgt
     2341 tccaggtctg gcttaagatt cccctgccca tgccctattt cggggtatag aagatggaag
     2401 tctttcagaa catggtcacc gggttcgcca tcgcgataac gccgatgaac ctggtgcttt
     2461 gtctggtagg gtgtttcgtc ggtacgttga tcggggtttt gccgggaatc ggcccggcgg
     2521 cgacaatcgc tcttcttctg ccggttacct tcaatcttcc tccggccacg gcaatcatca
     2581 tgctctcggg gatattttac ggcgccatgt acggaggatc gaccaccgcc atactggtga
     2641 acattccagg agaggcggcc tcggccatca cctgcctaga cggctatcaa atggcgcgac
     2701 aaggaagggc gggtccggcc ctgggtatcg ccgcctttgg ttccttcatc gccgggactt
     2761 tcggtgtctt cctcctgatg ctcatcgccc cgccggttgc tgacatggcg ttgaagttcg
     2821 gccctcccga aatctttgcg gtcatggtcc tgggccttac gatcctcaat tatttgagct
     2881 cggggtccct gatcaaggcc ttcatcatcg gactcatcgg cctgttcatc ggaatgatcg
     2941 gcatcgacat tgctggcatc taccggttta cctttggcat ccccgatttt tatgacggaa
     3001 tcgggctggt cccggtcctg atgggtttgt tcggagtggc tgaggtgctg ctcaacctcg
     3061 agcagatcgt cacccgccac atctttgaga cccgcatcaa gggcctcctt ccgaaccggg
     3121 aggactggaa gcgatcggcc atgccgatcc tccggggctc cttcatcggc tttgttatgg
     3181 gggttatccc agggccgagc aacaccatct cctccttcgt ttcttatgcc atggagaagc
     3241 ggctgtccaa atctcccgac cgatttggaa agggcgctat cgagggagtg gccgggccgg
     3301 agtcggccaa caactccgcc tgcgccggaa atttcatacc cgtcctcacg ctgggaattc
     3361 cgacgagtgc ggtcatggcg gtgatcgtgg gagcattcat catacacggg gtgacgccgg
     3421 gccccttgtt gatcaagaac aatccggaca tcttctgggg aaccatcgcc agcatgtaca
     3481 ttgggaacat catgctcctc attctgaatc tgcccctgat cgggatctgg gtgagggtcc
     3541 tgaaggttcc ctacggcatt ctcttccccc tgatcctgct gttctgcttc gtgggggcct
     3601 atgccaacaa caacagcctg gcggaactca atgtcatgat ctttttcgga ctggtggggt
     3661 acctaatgaa gaagaccggc tttgaaccgg cgcccctggt catggcctac atcctgtgcc
     3721 cgttcctgga agaggccttc cgccagtcgc tcatcaagtc ccacggagat ttttcaattt
     3781 tcatcaaacg gccgatttcc gccaccattc tggcaatagc cgcagggatc attgtcctga
     3841 acgtcttctc cggggtgaaa cagaggagaa aaagcctctt ggagaagata aaagaagaag
     3901 actgagtggg aatttatact tgcagggaaa attcacgaac cgtcctaagc ctataagcgt
     3961 tcatctttcc acgaggagga gaggaatgaa gactcgaaag cagcacatga tgatggtggc
     4021 cgtttgggta accctgtcct tgctcctgac cgcaaacggt ttcgcccagg aaaaatttcc
     4081 cagccggccc atccagttca tcatcccctg ggccccgggg ggcggaggga ccgtgaatgc
     4141 ccaggctctc cagcctcatt tcgaaaaagc gatcggagga agcgtgcaga tcatcaacaa
     4201 acccggggga ggagggacca tcgcttggaa ctacgtggcc aatgcggccc ccgatggata
     4261 caccgtgggg gttaccaacc agagctttat cttgacccaa tacaccaccc ggaccggcgt
     4321 cagcttcaaa aaagtcgatc ccattattat ggtcatcgac atgcccgccg ccctggcggt
     4381 gaaaaccgac gcgccctgga agaacttcaa ggagtacatc ggctatgcca aggccaaccc
     4441 gggaaaagtt cagatgggca actcaggaat gggggccaac taccatgtct ccgccctggg
     4501 aattgaaatg gttaccgggg tgaagcttac ccatgtaccc ttcaagggcg cgggcccgtc
     4561 gatcaccgct ctcctggggg gacacgtgga ttccgcggtg tcggagctcc ccactctcct
     4621 tccttatgtg gagggaaaga aattcaggct cctggtggtc ggctcccccg agagaaaccc
     4681 cgcggtcccc gacatcccca ccatgaagga agagggatat gagcagatat tgatcggggc
     4741 ctggtatgtc tggagcgctc ccaagggagt tccgaaaaac cggttccaga agctgcacga
     4801 cgccttcaag gcggcaatgg aagccccgga ataccgcgaa ttataccgaa agcttggggg
     4861 ggtggcgaga tatatggggc cgcaggagct accggccttt tatgagcaag aggacagagc
     4921 attgaaaaag atcatcgact tcagcgggtt taagccgatt gaataagact cgtaggggcg
     4981 ggtttcaagc ccgcccttac agcggcttga tcaaagcggc caaggaggag aggagatgaa
     5041 ccaaaaaaac ataagagtca taatcgcttt gacgggcgtt ctttcggtga tctttgcggg
     5101 gcccgtctgg gcccaggaaa aatttcccag ccgacccatc cagttcatca tcccctgggc
     5161 ggcgggaggg ggaggaacga tcaacgccca gtccttacag ccgcactttg aaaaggcaat
     5221 cgggggatcg gttcagatcg tgaacaagcc ggggggagcg ggcaccattg cctggaattt
     5281 cgtggccaat tctccgccgg acggctacac caccggaatc gttaacccca gcacggtagt
     5341 gaccctgtat accaccaaaa cgggcgtccc cctggaccgg ttcgacccaa tcgcttacac
     5401 cgtttacatc ccggcggggg tggtggtgag gactgaatct ccctggaaga cgttcagaga
     5461 attcatcgac cacgccaagg ccaatccggg gaaggtccag atggccaatt ccggccacgc
     5521 ggccatgtac cacatcggga tcgtgggcat tgagatggct atgggggtca agttcaccca
     5581 tgttccattc aaaggaacgg ggccctgcat caccgctctc atggggggtc atgtggacgg
     5641 gtccctgaac gaaatttcca cgcttcttcc ttatgtggag tccaagaaat tccggatcct
     5701 ggccgtttgt tcgcctaatc gcagtccggc cgtacccgac gcccccacct tcaaagaatt
     5761 cggctttgac cttgatgtgg gtacctggta tgcctatgtg gcggctaaag gaactcccca
     5821 agaccgactc aaaaaacttc aagatgcctt ccgggccggt gtggaatcga atgaattcaa
     5881 gagcctctac cagaagcagg gcggaatggc tatgttcaag ggtccagaag ccctagtggc
     5941 ttatttcaaa gagcaggacc ggctctggaa gaagatcatt gaatttgggg agtttaagcc
     6001 tgaataaatt tagtttcgag cttcgggttt gaggtttcgg gttaagacac cctattggca
     6061 ggtggatttt ctcggcccgg gactcgaaat ttgaaaccgg aaatttgcgt gaaaggggcc
     6121 ttgccttggg agcctctttt tcatgcagga gggggaagat gagattcttc agccgtttat
     6181 tgattttgtt cgggctgtgc tttttggctg cggccgggtc ggtgccgtca gcgggggcga
     6241 atgggagggt ggtcgtctac agctccaacc agcaagccca gaacgacatg atggcggaag
     6301 catttgaaaa agccaccggg gtcaagtgtg aaatggtgcg ggccggaacg ggcgtgctgg
     6361 tgaagaaaat gatagcggaa aaaaaccggc ctctggggga tgtggccctg ggcttgggca
     6421 agagcgtgct catcaacaac ctggacctct gggagcccta taaggtcaaa gatttttctg
     6481 cctacccatc ggagtacaag gacgccaacg gaatgtggat cggcaagatc gtccatctca
     6541 tggttttcat gtaccacacc aagctcctaa cccctgctca ggcccccaag gcctgggctg
     6601 atttccttga tccccgctgg cagaacaagg tcgctttctg caatcccagc aatgcagcct
     6661 cctcttacac tcaactgacc accatgcttt ccctctgggg ggacgaccag gcagggtgga
     6721 aaaaagtgga gacgttcctg aaacacgccc tgatcaccga gcagtccgca aacgtctata
     6781 cgaacgtggc aaacggagaa attccggccg gcatcaccat ggaatatgcc gcctatcggt
     6841 ataaaaaggg gggaggtcca attgaaatcg tcaacccggc cgagggcacc gtggcctatg
     6901 ccgaaggtct ggccattatc aagggagcgg cgaatagaga aaatgctcgc aaattcatgg
     6961 actgggccac cggcctggag gcccggaagc tggtcctctc ccagtttctg cgccgcccgg
     7021 cccgggaaga tatggacttt ggaaatctgg ccccgggaat ggtgcccatg agcaaaatca
     7081 agctgttgaa gagctttgac gaagactact ggatcaagaa gcagcccgag gttctccaga
     7141 aagtcaaaga cattcttctt cgggttaagt aggggcagtt cgcgaaccgc ccccagggag
     7201 ggttgcgggc aatttcttcc cttatcagtc catttaaatt gattgaggtc aaaatggaca
     7261 aattgtaggg gcaacccccc gtggttgccc atcttgcggg cggccacagg gggccgcccc
     7321 tacaataacc tgtttggttc tggctctgcc ataagaaatt gcggctgaat ggaggtgcaa
     7381 agtgaaaatc gacatcagcg ccgacgtagg agagagcttc ggccgctgga aactggggta
     7441 cgacgaagag ctgatggaat acatctcttc ggccaatctg gcctgcgggt tccatgcggg
     7501 cgaccccatg gtcatgcgga atacggtgaa actgtgcaaa aagtatgggg tgaaggtagg
     7561 aacccatgtg ggttttccgg acctcatggg ttttgggcgg aggcttatgg ctctcacccc
     7621 cgaagaatac ctgaattact ccctttacca gggaggtgcg ctccaggcga tcgcccgcgc
     7681 ggaaggagaa gaactgcagc atttcacttg gcacggcagc gcagggactt ccttcaacgc
     7741 tgacaacgag gagatcgccc gagccgggat cgcggctgtg gcccagcttg acaggaatat
     7801 catcgtcccc ctgatctacg gtccgaaagg agacttgatg gcccgggaag cgggcaaagc
     7861 agggctcaag gtggtgagaa agttcttcgc cgaccgggct ctggaagcca acgggatgct
     7921 tgtatcccga aagaaggccg ggtcggtcat taccgatgcc gaagaatgcg cggcccgcac
     7981 cctgaggatg gtccaggacc ataaggtgaa aaccatcaac gggaaggata tcgactgctt
     8041 cggccagacc atcatggtcc acggggatac ccccacagca gtcaaggtgg cccaaaccat
     8101 ccggaatcgg ttggagggcg ccggaatcca gattgtcccg atgaaagaat tgatttgatc
     8161 attggaagca gatgaagaca gattttcagg attcaaaaaa aggaaacact tttgcttttt
     8221 gaaaaaagct ccctatttct ccatttctcc tttttaacca agggatctgt cagaaatccc
     8281 cccgttcccc cctttgaaaa aaggggggca gaggggggat ttaaaaaggc cattttagga
     8341 atgtttatat aaaaaaagag gtctcttgac tttaatttgt tttttcagcc acaaatcggc
     8401 gtttatctgc gttcatctgc gtccgatgga tggaaaaaat gagtgctttt gaagtaattc
     8461 aaccgggcgc tttcacgacc gtacaagacc tggggaggta tggataccag aagtacggag
     8521 tctccatctc cggggccatg gaccggttcg ccctacgcgt agccaacctc ctggttggaa
     8581 acgatgaagg agagggggtc atcgaggcga cgatcattgg ccccaagtta aaggccttag
     8641 aaagaatgcg ggtagcgatt accggcgctg acctttctcc ggagttcgga ggaaaaccct
     8701 tgcctctatg gcgaactctg gatattcccg agggaggtac cctatctttt ggagcgccga
     8761 ggagcgggtg ccgtgcttac ctggccgtcg ccgggggaat tgacttcccc gtggtcatgg
     8821 gaagccgctc cattcacacc cgttccaacc tcgggggaaa tggacggact ttggtgaaag
     8881 gcgatattat agaaagaaag gattcaggat tcaggattca ggaatcagaa acccatcgat
     8941 tgctcccgga actggtccct gtttatggaa agaattggca gatcagggta atcttcggcc
     9001 cccagaagga ttatttcacc cggcagggga tcgagacttt cctcaccagt gaatacgaga
     9061 ttacccccca ggccgaccgg atgggatacc ggctgaaagg cccgaagatc gagcacaagt
     9121 ccggggcgga cattctcacc gatgccacgc ccccgggttc tatccaggtc cccggggacg
     9181 gaatgcccat tattcttttg gccgatgggc agacgaccgg tggatattcc aagatcgcga
     9241 cggtgatatc tgtagaccag gacctgcttg cccaagccag gccgggggaa aaggtccggt
     9301 tccagaaagt tacgatcctt gaagcccatc aatttcttcg ggagatggaa gagaaaatcc
     9361 agagaattaa gaaagctttg atcccggtat gagcggctat ccaaaggccc gttacctcct
     9421 cgtcggcgac catggaatct ccgtggaatt tggaaattcc ctggaagagg agatcaacct
     9481 caaagtccaa aagctgatgc acgctctgga gaaggccaag atccccgggg tcatcgaaac
     9541 gaatcccaca attcggtctc tttttgtata ttatgatccc cttcggatcg gggtgagaaa
     9601 attgattaag gagatgagaa agatggaagg acggatggct gatatctttt tgccgaagtc
     9661 ccgtcttttt gaagttccga ttgtttatgg cggaaagtat ggacccgatc ttgaagaggt
     9721 tgcgaaactg ctcaacctga caccggaaga gatcatccgg attcatagcg ggaaagagta
     9781 tttcatttat gatacgggtt ttatcggcgg gtcggcccat ttcaaggtcc cgccacccct
     9841 ggatttgctg ccgcgaaaga aaactcccaa cctgggagtc cctgcagggg cagtactgat
     9901 cgccggtggg ctggggagcg tttataaacc cctggctggc cccacgggtt ggtattggat
     9961 cggcatgtct cctcttaagc aatggttccc gcaaaaggac cctccccttt tgattcatcc
    10021 gggagataag gtcatctacc gtcccgtgga cgaggaagag ttcgatcgga ttaaaaagag
    10081 ggtggaggaa ggtaatttta ctgctaaaat cctgtcctga ggggcaccga aaattcaagc
    10141 ggataaattg aaaatcaagt agtatcccta taggcgttga tatattatgc ttttgagaat
    10201 ttttttcatc cagtcattga gaggcgcgta
//
LOCUS       DASPRR010000270         3504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1351608, whole genome shotgun sequence.
ACCESSION   DASPRR010000270 DASPRR010000000
VERSION     DASPRR010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351608"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /gene="rnpB"
                     /locus_tag="VEL68_10595"
     ncRNA           <1..187
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEL68_10595"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            741..1427
                     /locus_tag="VEL68_10600"
     CDS             741..1427
                     /locus_tag="VEL68_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36457.1"
                     /translation="MGDQEVFRKLMDDKEIFLKISPALFFEVLLRKAANDLEEAGYTL
                     EKTSRMKIPVFDTKEVSKLLTREPLLLYLADMLASFTKIQSYTLSFRVRKGVWERIRF
                     NDLDILSLMNFCQAAGDEYRIGFYKRIADICLFILGIFPDYAERDYRYPFSGQVRPHI
                     HGKLRISPEDYEKEGRKFYKLAAEHPSAKEMDLAETFWALYENFQKAKKPLNFISDHY
                     LQYTRHKFLI"
     gene            1670..3025
                     /locus_tag="VEL68_10605"
     CDS             1670..3025
                     /locus_tag="VEL68_10605"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXZ36458.1"
                     /translation="MIGKEVALKMLQRLVQGSPADQTEALLLTEDSSLARFIPTGIHQ
                     HVAERNRTLILRVVLGKRMAVATTNILHPSSLKILMQEAIALAKIQPANEAFVSLPGP
                     KPIPAMDTFSKNIRHLTPGRKAKIIQEVMSRVQENGCSASGAFSHGEVELAVINSLGV
                     EAYQQFSDLFLHLIVQNEKGSGYASFVSRDWDQLRVEPLVNAAVAKASRGNPVQVEPG
                     EYEVIMEPQAVQELLVFLAYLGFHGLAVQEGRSFFCNQFGKKMVHEKVTIYDDGLEPQ
                     GLPVPFDFEGVPKQKVIFFEGGVAKNVTYDSFTAGREGKESTGHGLIPPNTEGPVPVN
                     LFMQAGETHLEEMIRSVRRGIYVTRFHYTNAVDPMKAVITGMTRDGTFWIEEGEIKNP
                     IKNLRFTESILKALSRVKAISRERKLCSSGSVYGRRFITGTVVPAIQVDGFNFSGVSS
                     M"
     gene            3063..>3504
                     /locus_tag="VEL68_10610"
     CDS             3063..>3504
                     /locus_tag="VEL68_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HXZ36459.1"
                     /translation="MKDLTENVLSLTRLKNADYADLRVVRRRWEEILVKNGNIEAVTH
                     DEDFGFGVRVLFQGAWGFACSSKVNKKEVERTFDRAWKISREGSRIQGKRPFFPPASP
                     VVARYQTPFSIHPLQVSMEEKLKYLLEAERTIARNRRVNISEAFM"
BASE COUNT          829 a          897 c          964 g          814 t
ORIGIN      
        1 ctggtgacag ggctggcaag gtaaacccca cccggagcaa gaccgaatag aggagcgctt
       61 gagagtggcc cgctcgatgc tcccgggtag gttgctagat cccggcagca atgtcggggc
      121 cagaggaatg atcgccctct ccggtcttcc ggagagacag aactcggctt acagacccgc
      181 ttccgcggtt tcttctcatc tggtcgcgct tgtccggaga ccttaggtgc gattcagcgg
      241 tcgcccctac cttaccccta taatcttcca gaagggatct tacccaaagc ccatctacga
      301 attcgaaggg aaaaggaaca agaattgggg aaccctcctc cctgcgtccc gcggcggttc
      361 tcatcggcgg agtgaccctc ggggaaaacc gttacatcgg ccccgggggc tgtactgcgc
      421 caaacccttc ggcggccaac gcgcctggcg cgatgcgcgt tcgtagcggg cggattatcc
      481 atctcatgga ttccctttga aaaaaaatcc attatatttt taggaatcgc ctaaacattc
      541 ggaaggaaaa taacgataag taagcaagat gcctataggg gatctttgga agtgagggaa
      601 ggaaaatgac tccgtttaaa ttaaatcggt tatctgacag ggacttggat ttcctggcag
      661 agactgcatc tcccgaaatt caagataaat cccgtctcaa ggagatcatc caggaagatg
      721 aggattttag aaattcgtat gtgggagacc aagaagtatt ccggaagctg atggatgata
      781 aagagatatt tttgaaaatc tcccccgcgc ttttctttga ggtcttgctg cgcaaggccg
      841 ccaatgacct ggaagaggcc ggttacaccc ttgaaaaaac cagccgcatg aaaattccgg
      901 tattcgatac gaaagaggta tcgaagctct tgacccggga gcctctgctg ctctatctcg
      961 ccgacatgtt ggcttctttc actaagattc aaagttacac cctctccttc agggttcgga
     1021 aaggggtctg ggaaagaatt cggttcaatg acctggatat cctcagcctt atgaatttct
     1081 gtcaggcggc gggggatgag taccggatcg gtttctataa aaggattgcc gatatctgcc
     1141 tttttatcct gggcatcttc cctgattatg ccgagcggga ttaccgttat cccttttccg
     1201 gacaagtcag accccatatt cacgggaagt tacggattag tccggaagat tacgaaaagg
     1261 aaggccgaaa attctataaa ctggccgcgg agcatccatc cgccaaagaa atggacctgg
     1321 ctgagacctt ttgggccctc tacgaaaatt ttcaaaaggc caagaagccg ttgaatttta
     1381 tctccgatca ttaccttcag tatacccggc ataagttcct tatttaggct ttccaccgca
     1441 atcggtccct gaagccatct tcttctcctc ctatggtatt ttggtcggcc cgagacttat
     1501 cgtcatctcg caaggtctgt attctatagt ctgcggccta ggcctcatga atgccaggat
     1561 tcagccgggc agaagctcta tcccggtctt aggctttcag ctataattga gtaaaagcgt
     1621 ctcaggccgc ccattcatat ttcacgaatc caaagaggga gcctcaagaa tgatcggaaa
     1681 agaagtcgcc ttgaaaatgt tacagagatt ggttcagggg tcgccagccg atcaaacaga
     1741 agcccttttg ctgaccgagg actcttccct ggcccgcttc attcccacgg ggattcatca
     1801 gcatgtggcc gagcggaacc ggacgttgat cctgcgggtt gttctgggaa agcggatggc
     1861 cgtggccaca acgaatattc tccatccctc ttcgctcaaa attttaatgc aagaggccat
     1921 cgcgctggcc aagatccagc cggccaatga ggctttcgtg tccctgcccg ggccgaagcc
     1981 tattcctgcg atggacactt tttccaaaaa tatccgccat ctcactccgg gaaggaaggc
     2041 aaagattatc caggaggtta tgagtcgggt gcaagaaaat ggctgtagtg cctcgggggc
     2101 tttttcccac ggagaggtgg aactggccgt gatcaactcc ttgggcgtgg aggcttacca
     2161 acaattttcc gatttattcc tgcacctgat tgtgcagaat gagaagggct caggttacgc
     2221 cagcttcgtt tcccgggatt gggatcaact gcgggtagag cccttggtca acgcggcggt
     2281 cgccaaagct tcccggggga acccggttca ggtggaaccc ggcgagtacg aggtgataat
     2341 ggaaccgcag gcggtccagg agctcttggt ttttctggcg tatctgggtt ttcatggact
     2401 ggcggtccag gaaggccgga gctttttctg caaccagttt ggcaaaaaga tggtccacga
     2461 aaaagtcacg atttatgatg atggcctgga gccccagggg ctgccggttc cgttcgactt
     2521 tgaaggggtg cccaagcaga aagtcatttt ctttgaggga ggggtggcca agaatgtgac
     2581 ctatgactct ttcactgcgg gccgggaagg caaggagtcc accggccatg gcctcatccc
     2641 cccgaacacc gagggaccgg tcccagttaa ccttttcatg caggcgggag aaacccatct
     2701 ggaagagatg attcggtcgg tgcggcgggg aatttacgtg actcgtttcc attacaccaa
     2761 cgcggtcgac cccatgaagg ccgtcatcac cgggatgacc cgcgacggaa ccttctggat
     2821 cgaggagggt gagatcaaga atccgatcaa gaatctccgc tttaccgaat cgatcctgaa
     2881 ggctctttcc cgagtgaagg cgatctcccg agaaaggaaa ctctgctctt cgggttcagt
     2941 ctacggcagg cgcttcatca ccggcacggt ggtccctgcc attcaggtgg atgggtttaa
     3001 tttttccggg gtatcgtcca tgtaaccggc ccgcgaggat gcgggggatg ctggaggctg
     3061 agatgaaaga tctgacagaa aatgttctct ctctgacgag actgaaaaat gcggattatg
     3121 cggatctccg cgttgtccgc cgccgttggg aagagatcct ggtgaaaaac gggaatatag
     3181 aggctgtcac ccacgatgaa gatttcgggt tcggggtccg ggtccttttc cagggcgcct
     3241 gggggtttgc ctgcagctcc aaggttaata agaaagaagt ggagaggact ttcgaccggg
     3301 cctggaagat ctccagggaa ggttcaagga ttcaggggaa aagacccttc tttccccccg
     3361 cctctccggt tgtcgcaagg taccagaccc cgttttccat ccatcccctc caggtctcga
     3421 tggaagaaaa attaaagtac ctcctggaag cggaacggac catcgcccgg aatcgaaggg
     3481 tgaatatctc cgaggccttt atgg
//
LOCUS       DASPRR010000271         7795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1351726, whole genome shotgun sequence.
ACCESSION   DASPRR010000271 DASPRR010000000
VERSION     DASPRR010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351726"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VEL68_10615"
     CDS             complement(<1..205)
                     /locus_tag="VEL68_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36460.1"
                     /translation="MTNFPLHFALLIMGFTFTISQVMVIRELLVVFAGNELSIAIILA
                     NWLLLEAIGSFFVGRKVGEWGLRE"
     gene            185..1096
                     /gene="recO"
                     /locus_tag="VEL68_10620"
     CDS             185..1096
                     /gene="recO"
                     /locus_tag="VEL68_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXZ36461.1"
                     /translation="MKREVGQADLPEEGICSKRNYSTPPPSHPLSFLLRFLMPLYKTP
                     AVILHTLPYGESDQIVTFYTLDFGKVKGIAKGAKRSRKRFGVRLEIASYVKVTFFEKE
                     TSSLVRLSNCDLIRPFDGLREDIKKLAWASYFIELVNEMTAEKIQNKPLFRLLVAFLT
                     FINKGMLEEEIQRVFEIRFLSCLGYQPQLEHCTHCQKKLSGERFFFSPRKGGVICSAC
                     AASLSGLIPVSLGTIKTLLLAHTLPLEKVSRISFSPQSLKESLAILSSFLQQYLGKEL
                     KSRKFLEQITPNAEQTIITKSQAANSK"
     gene            1288..2166
                     /gene="glyQ"
                     /locus_tag="VEL68_10625"
     CDS             1288..2166
                     /gene="glyQ"
                     /locus_tag="VEL68_10625"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXZ36462.1"
                     /translation="MYFQDLIMALQKFWAERGCILQQPYDIEVGAGTFNPATFLRVLG
                     PEPWRVAYVEPSRRPADGRYGENPNRLQHYYQFQVILKPSPLEIQDIYLNSLKAFGVE
                     PLEHDIRFVEDDWESPTLGAWGLGWEVWLDGMEITQFTYFQQAGGIDLKPISVEITYG
                     IERIAMYLQGIDNVYDLEWTRGIRYGDVHHRGEVEFSTYNFEQADVPMLFQLFDMYEK
                     EATRLNEKGLVLPAYDYCLKCSHTFNLLEARGAISVTERTQYIARVRNLARISAEGYL
                     KQRESMGFPLLRKDDA"
     gene            2333..4399
                     /gene="glyS"
                     /locus_tag="VEL68_10630"
     CDS             2333..4399
                     /gene="glyS"
                     /locus_tag="VEL68_10630"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXZ36463.1"
                     /translation="MSELLLEIGTEEIPSGFIPKALEEMQSLLEKAFQAQRIEYKDVK
                     AMGTPRRLVVVATGVASAQEGRIVETIGPAKRIAFDPQGNPTKAALGFARGQGIPVEE
                     LKTVQTEKGEYVCARKEEKGEETFPLLSGILPRLMASIPFPKSMRWMDLDNSFARPIH
                     WILALFDGKVIPFQVGNIASGNVSTGHRFMALGSFQVKDLADYLRRLKTSFVIVNPEE
                     RRDLILAEVNKAASEVSGHISKDDDLLEIVTYLVEYPVALRGSFGKEFLSLPREVLIS
                     AMREHQRYFSVCDSTGALLPYFVAISNTKPRDPNMVIRGNERVLRARLSDAQFFFFED
                     QKVPLLQRLEGLKKVVYHSKLGTSYEKVMRISSLADFLAHRIDPSLLETVKRAAQLCK
                     GDLITGMVGEFPSLQGVMGQVYARLSGEKEDVALAIYEHYLPTAAGGALPTSRPGAIL
                     SISDKLDTIAGCFGIGLIPTGTADPYALRRQTLGIINIILDQHFSLSLSEVIKKSLSF
                     LKKKIERPPQEVKADVLDFFKGRIQNLLVGRGISADVVEAASAAGLDDLVDLEKRAQA
                     LHDLKKEPDFEPLAVAFKRVVNISKSHPPGPVSPKCFENPAEDRLLEAYRQIGQQAEK
                     SIKSKDYPSALKKLSLLRKPVDEFFDAVMVMVEDEKTKTNRLSLLANISKIFFKIGDF
                     SKITTA"
     gene            4887..>7795
                     /gene="smc"
                     /locus_tag="VEL68_10635"
     CDS             4887..>7795
                     /gene="smc"
                     /locus_tag="VEL68_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HXZ36464.1"
                     /translation="MKIKRMEVLGFKSFAAKTQIEFPPGVTAVVGPNGCGKSNVVDAI
                     RWALGEQSPKQLRGKAMEDVIFNGTEAQKPLGMAEVTLVFSNENGKPLAEYLDYSEIA
                     VSRRLFRSGESEYLINKVPCRLKDITDLFLGTGVGHRAYSIVEQGKMEFVLNAKPEER
                     RILIEEAAGITKYKDRKAAALRKIEATQQNLLRLSDVIGEVKRQMNSLNRQAKKAERY
                     KGYRDEMRLIELGQAHQTYRSLQSQHEEMQASLQGIKDLEVQAAAQIQEVEAEVERIK
                     VNLLDLERDLTVHQERLSENEGTIKTRENQIELSSRDRENLQKQAFRAEEEVGKLTHQ
                     IEEANREVQGYEESLQELQQKIACDADALFEKERIFSDKKTQLNDHEQALSQEKNILV
                     DLLTRLAHLKNQLLDLNRRSGELLRRQEKLAGERAEAEKKAAELRHHMVQQSLQLTDR
                     RNARSQVEEQKEGKIAQLKEVQSTLAALQEDLNQQKERLNRESSRLNSLLELQRNFEG
                     YQEGVRAILLKRQAQGMDQNGICGLVEDIIETDPQYECVVESVLGEKLQHFIVQNHEE
                     SLKAIDYLKAQGSGRSSFIPLQVKSNPLPSPSVSPNGAITPLLNVVKVKPEFTHLAQY
                     FFGDVWIVSNLRQAIDLWNQDGIWKILVTLDGEVLHPSGVVTGGSREQVSSGTFHRKR
                     EIRDLTHSTEELRKAIASREEKQEELLGEIKILESAIEEMIQALHQEEIRIVSEAKEL
                     DQGELELKRWRQEIETLRFEESQLAEERGEVQTQTHQNETALREAEQEKQSREEGLIQ
                     REQDLQTLKGDIEGLMAELTEAKLRVATLQEKKQNLDQNMERARQVLQENESVLLSRR
                     EEVEDCLRQIKEAEEKRRQAESDLNRLLSEHQELHSYLDKKRETLREEKEKLDKSEVL
                     EKERRESLHHLHEQKNTLSMKLMETDLNLKHLLSAVEEKHRIPAEAFLSREDDQD"
BASE COUNT         1994 a         2144 c         2055 g         1602 t
ORIGIN      
        1 cttctcgaag gccccattcc ccaacttttc tcccgacgaa gaaactgccg atcgcctcca
       61 agagaagcca gttggccaga atgatggcga tggacaactc gtttccggcg aagaccacca
      121 gcagctcgcg gataaccatc acctgagaga tggtaaaggt aaaccccatg ataaggaggg
      181 cgaaatgaag agggaagttg gtcaagccga tctccccgaa gaaggtatat gttccaagcg
      241 aaattatagc acgcctcctc cttcccatcc cctttccttt ctgttaagat ttctcatgcc
      301 cctctacaag actccggcgg tcattctcca cactttgcct tacggcgagt cggatcagat
      361 cgtcaccttt tacaccctcg acttcgggaa ggtgaaggga atcgccaagg gagccaagcg
      421 gtcgcgaaag cgattcgggg tccgactgga gatcgcctcc tatgtgaagg ttactttttt
      481 tgaaaaagag acttccagcc tcgtccgcct gagcaactgc gacctgatcc gccccttcga
      541 cggtctcagg gaagatatca aaaaactggc ctgggccagc tatttcatcg agctggtcaa
      601 tgaaatgacc gcagagaaga tccaaaacaa gccccttttt cggcttttgg ttgcctttct
      661 gaccttcatc aataaaggga tgctcgagga agaaatccag cgggtttttg aaattcgctt
      721 tctctcctgc ctgggctacc agcctcagct cgaacattgc acccactgcc aaaagaagct
      781 ttccggggag agattcttct tcagtccccg caaaggcggg gtcatctgct ccgcttgcgc
      841 agcgagcctt tccggcctga ttccggtttc tttgggaacc atcaagactc tgctcctcgc
      901 ccataccctc cccctggaaa aggtaagccg aatctccttc tcccctcaat cccttaagga
      961 aagtctggcc atcctttctt cctttctcca gcaatacttg ggcaaagaac tcaagtcgag
     1021 aaaatttctg gagcaaatca cgccgaatgc ggaacaaaca atcatcacca aatcccaagc
     1081 agcaaattcc aaataaattc gaatgcccaa aattccaagt ccaaatccct caataagagg
     1141 gtctaatcca acggtcctga taattggatt ttgaaagtta ttcatatttt ggaatttggt
     1201 gtttcaaccg ttgtatttac cttgactttt gtttccttta aatgctagat tttttattta
     1261 ttaaaataat ttcaaaggtt tctagtcatg tattttcaag acttaatcat ggccctgcag
     1321 aagttctggg ccgagcgggg ctgcattctg caacagccct atgatattga agtgggagcc
     1381 gggactttca acccagccac ctttttacgg gtgctgggtc cggagccttg gagagtcgcc
     1441 tatgttgagc catcccggag gcccgccgat ggccgctacg gggagaatcc caaccgcctc
     1501 cagcattatt accagtttca ggtcatcctg aaaccctccc cgctggaaat ccaggatatt
     1561 tacctgaaca gcctgaaggc tttcggagtc gagcccctgg agcacgacat acgcttcgtg
     1621 gaagacgatt gggaatctcc cactctggga gcctggggat tgggctggga agtgtggctc
     1681 gacgggatgg aaatcaccca gtttacctat ttccagcagg ccgggggaat cgacctgaag
     1741 cccatctctg tggagatcac ctacggcatt gagcgcattg ccatgtacct gcagggaatc
     1801 gacaacgtat acgacctgga gtggacgcgg gggatcagat acggcgatgt gcatcaccgg
     1861 ggagaagtgg agttttccac ctataatttc gagcaggccg atgtgcccat gctcttccag
     1921 ctcttcgaca tgtatgaaaa ggaagccacc cgcctgaacg agaaaggact ggtccttccg
     1981 gcgtatgatt attgcttgaa gtgttcccac acctttaact tgttggaggc caggggggcg
     2041 atcagcgtca cggaacggac ccaatacata gccagggtgc gcaacctggc ccgaatttcg
     2101 gctgaggggt acctcaaaca gcgcgaatcc atgggtttcc ctctcttaag aaaagatgat
     2161 gcttgactcg ggacgaagat aaacgcagat ttccaggata tggacgaaac gaccaaacca
     2221 aatttttttg cccaacttga aaggaaaatg atgcctccgg atttcttttt taattttctt
     2281 tttgatctgc gtatttctgc gaaaatctgc gtccccttta aaaaggtctt ctatgtctga
     2341 actgctgctg gaaatcggaa cggaagagat cccttcgggt ttcattccca aagccctgga
     2401 agagatgcaa agcctgttgg agaaggcatt tcaggctcaa cgcattgaat ataaggatgt
     2461 caaagctatg ggaacccccc ggcggctggt cgtggtggcc acgggagtcg cttctgccca
     2521 ggaaggaaga atcgtcgaaa cgatcgggcc ggccaagcgt atcgccttcg acccccaggg
     2581 gaaccccacc aaggcagccc tggggtttgc ccggggccaa gggatcccgg tcgaagaatt
     2641 gaagaccgtc cagactgaga agggagagta cgtctgcgcc cgcaaggagg agaaggggga
     2701 agagaccttt ccccttctct ccggcattct gccccggctg atggcctcga ttcccttccc
     2761 caaatccatg cgctggatgg acctggacaa ttccttcgcc cgccccatcc actggatcct
     2821 ggccctgttt gatgggaaag tcattccctt ccaggtcggg aacatcgcct cgggaaatgt
     2881 ctccacggga caccgcttta tggccctcgg ttctttccag gtcaaggacc tcgccgatta
     2941 tttgaggcgt ctcaagacct ccttcgtgat tgtcaacccc gaggagcgca gagacctcat
     3001 cctggccgag gtgaacaaag cggccagtga agtctccggc catatttcca aggatgacga
     3061 ccttctcgaa atcgtcacct acctggtgga atatccagtt gccctccgcg gaagttttgg
     3121 caaagagttc ctttcccttc cccgggaagt tctgatcagc gccatgcggg agcaccagcg
     3181 ctatttttcc gtttgcgact ccacgggcgc ccttttgccc tatttcgtcg ccatctccaa
     3241 caccaaaccc agggatccca acatggtgat ccgggggaac gagcgagtcc ttcgggcgcg
     3301 cctgtccgat gcccaattct tctttttcga agaccagaag gtccccctcc tccagcggtt
     3361 ggaggggttg aagaaagtcg tctaccactc caagctgggc acctcctacg agaaggttat
     3421 gaggatatcc agcctggctg attttctcgc ccatcgcatc gatccatccc ttctcgagac
     3481 ggtaaagagg gccgcccaac tctgcaaggg ggatttgatc accgggatgg tgggggaatt
     3541 cccttccctc cagggggtga tggggcaggt ctatgcccgc ctctccgggg agaaggaaga
     3601 tgtggccttg gccatctacg agcactacct acccacggcc gcaggggggg ctctcccgac
     3661 ctctcggccg ggagccattc ttagtatatc cgataaactg gacaccattg ccggatgctt
     3721 cggcatcggc ctcatcccta cgggaactgc cgacccctac gccttgagac gacagaccct
     3781 gggaatcatc aacatcatcc tggaccagca cttctctttg tccctgagcg aggtcattaa
     3841 aaaaagccta tcctttttaa agaagaaaat tgaacgcccc ccgcaggagg ttaaggcgga
     3901 tgttcttgat ttcttcaagg ggcgaatcca aaacttgctc gtcggccgag gaatttcggc
     3961 ggacgtggtc gaagccgcat ccgcagcagg cttagacgac ttggtggacc tggagaagag
     4021 agcgcaggcg ctccatgatc tgaagaagga gccggacttc gagccactgg ccgtagcctt
     4081 caagcgggtg gtcaacattt ccaaatctca cccaccgggt ccggtttccc ctaaatgctt
     4141 tgaaaacccc gcggaggaca ggctccttga ggcgtatcgg cagatcggcc aacaagccga
     4201 aaaaagcatt aagagtaaag actatccttc tgccctgaaa aaactctccc tcctccggaa
     4261 accagtggat gaattctttg acgcggtcat ggtcatggtc gaagatgaaa agaccaagac
     4321 taaccgcctc tcccttctgg ccaatatctc taagatcttt ttcaagatcg gggacttctc
     4381 caaaatcaca accgcataaa aatcgggttg gaggcagaag gtttgaggct gggggttaga
     4441 ggaaggtttt tgcctccaac ctctaacctc aaacctccaa catttttttc tttcatccct
     4501 agatgtttaa atctattttt ctcttcaaat ggcccctagc taaattcgaa cttccctatc
     4561 ccattgagga tcaattaatc aaaagaattc cactacatat tgtggagaat gggctcaggt
     4621 atacatttag ttgtattttt ttaactttac aaaaaagacc atccctgata gaattgcccc
     4681 taacaaggaa ggactccatg tactagaatc attcgtgcct ctggcatgac tcttgaaagc
     4741 aagagggaag cgacattccg tgcatgcgaa gccctgcacc gcatggtgtt cagcgcaaag
     4801 cgcagccctg aattacggag aaggtggctt tgcggggagc tcaattgaaa tcatttattt
     4861 tttccagttg aagcgaaggg gtcgccatga agataaaacg gatggaagtc ctagggttca
     4921 aatcttttgc cgcgaagacc cagattgaat ttcccccggg ggtgacggca gtagtaggac
     4981 ccaacgggtg tgggaaatcg aacgtggtgg atgccatccg ctgggccctc ggggaacaga
     5041 gccccaaaca actccggggg aaggccatgg aagatgtgat cttcaatggc acagaggccc
     5101 aaaagccgct aggcatggcc gaagtcaccc ttgttttctc caatgaaaac ggaaaacccc
     5161 tcgccgagta tctcgactac agcgaaatcg cagtcagccg gaggctcttc cgctcggggg
     5221 agagcgaata tctgatcaac aaggttccct gccgcctcaa ggacatcacc gaccttttcc
     5281 tgggcaccgg ggtcggccac cgggcttact ccatcgtcga gcaagggaag atggaattcg
     5341 tcctgaacgc caagcccgaa gagcggcgta tcctcatcga ggaagcggcg ggcattacca
     5401 aatacaaaga ccggaaagcc gccgccctcc gcaagatcga ggccacccaa caaaatcttc
     5461 tccgtctgag cgatgtcatc ggggaggtca agaggcagat gaactccctg aatcgccagg
     5521 ccaaaaaggc ggaacgttac aagggatacc gcgatgagat gcggctcatc gagttgggac
     5581 aggcccacca gacctaccgc tccctgcaaa gccagcacga ggaaatgcag gcttcgctgc
     5641 agggaataaa agacctggag gtccaggctg ccgcccaaat ccaggaggtg gaagccgagg
     5701 tggagagaat caaggtcaac ctcctggacc tggaaaggga cctcaccgtt catcaggagc
     5761 gcctctccga gaacgaaggg acgataaaga cccgggagaa ccagatcgaa ctttcctcgc
     5821 gggatcggga aaacctccag aagcaagcct ttcgggccga agaagaggtc ggaaaactga
     5881 ctcatcagat cgaggaggcc aatcgtgaag ttcagggata cgaagaatcc ctgcaggaac
     5941 tccaacagaa gatcgcctgc gatgccgacg ctctgttcga aaaggaaaga atcttctccg
     6001 acaaaaagac ccagctgaac gaccacgaac aggccctctc ccaagaaaag aatattttgg
     6061 tggacctcct cactcgtttg gcccatctga agaaccagct tctggacctg aatcgccgat
     6121 ccggagagct cctccgcagg caggaaaagc tggccgggga aagggccgaa gcggagaaaa
     6181 aagccgcaga actccgccat cacatggtgc aacagtccct ccagctcact gaccgccgga
     6241 atgctcggag ccaggtagaa gaacaaaaag aaggaaaaat tgcccagctt aaagaggtcc
     6301 agtccaccct ggccgccctt caggaagacc tgaaccagca gaaggaacgg ctgaatcggg
     6361 aaagctcccg gctcaactcc ctcctggaac tccagcgcaa cttcgaaggg tatcaggaag
     6421 gggtccgggc catccttctc aagaggcagg cccaggggat ggatcagaac ggcatctgcg
     6481 gcctggtaga agatataatc gagaccgatc cccaatacga atgcgtggtc gagtcggttt
     6541 tgggggaaaa gctccagcat ttcatcgtcc agaaccatga agagagcctc aaggcgatcg
     6601 actatttgaa ggcccaggga tcgggccgct ccagcttcat cccccttcaa gtgaaatcca
     6661 acccgcttcc ttccccttct gtttccccga acggggctat cacccccctc ctgaacgtgg
     6721 tcaaggtgaa accggagttt acccatttgg cacaatattt cttcggagac gtctggatcg
     6781 tctccaacct gcgccaggcc atcgatctct ggaaccagga tggaatctgg aaaatcctgg
     6841 tcaccttgga tggggaagtg ctgcacccct cgggagtggt cactggaggg agcagggagc
     6901 aggtctcttc cggaactttc catcgcaaaa gggaaatccg ggacctcacc cacagcactg
     6961 aagagctccg caaagccatc gcctcacggg aagaaaagca ggaggaactc ctcggagaaa
     7021 taaaaatcct ggaatcggcc atcgaggaaa tgattcaggc cctccaccag gaggaaatcc
     7081 ggatcgtcag cgaggcgaag gagttggacc agggagaact ggaattgaaa cgctggcggc
     7141 aagagatcga aacgctccgg ttcgaggaga gccagctggc cgaagagcgg ggcgaggttc
     7201 aaacccagac tcatcagaac gaaaccgccc tccgggaggc ggagcaggaa aaacagagtc
     7261 gggaagaggg gttgatccag cgggaacagg acctgcaaac cctgaaaggg gacatcgaag
     7321 gcctcatggc cgaactgacc gaggccaagc tgcgagtggc caccctgcag gaaaagaaac
     7381 agaacctgga ccagaatatg gagcgggccc gccaggtcct acaggagaac gaatccgttc
     7441 tcttgagccg gcgggaagag gttgaggatt gcctccgcca gataaaggag gcggaagaaa
     7501 aacggcggca ggcggagagc gacctgaacc gtctattgtc ggagcaccag gaactgcatt
     7561 cctatctgga taagaagcga gagactctcc gggaggaaaa agaaaagctg gacaagtcgg
     7621 aagtcctgga aaaggagagg agggaaagcc tgcatcatct ccacgagcag aagaacaccc
     7681 tatccatgaa gctcatggag acggacctca acctgaaaca tctcctttcc gccgtggaag
     7741 aaaagcaccg aatccccgcc gaggccttcc tctcccggga ggacgatcag gatta
//
LOCUS       DASPRR010000272         4927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1510088, whole genome shotgun sequence.
ACCESSION   DASPRR010000272 DASPRR010000000
VERSION     DASPRR010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510088"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VEL68_10640"
     CDS             complement(<1..294)
                     /locus_tag="VEL68_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HXZ36465.1"
                     /translation="MKKTYLHPLPLRIWHWVNALLVILLIITGIQLRISGVPSLGPRH
                     PALWLHRYAGWAMVASCVFWLVYSLASRNLSRQYAFRARDFKGMFAQAKFYLIS"
     gene            complement(291..1211)
                     /locus_tag="VEL68_10645"
     CDS             complement(291..1211)
                     /locus_tag="VEL68_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ36466.1"
                     /translation="MMEEHVEKKEILESDLGSLSLNPLCSRRQTLKWGGLSFIGISVL
                     GPVLAQGATKPLILMEQAQGLVVGDPMKCVGCGRCELACTEFNDGKSAPAMSRIKVWR
                     NMNFGLGGVAAWREGEGNWGDGLVVQDLCKQCPHPVPCANICPENAIVLAPSGNARMV
                     DSEKCTGCKVCLKACPWEMISFDPDTRKATKCHLCNGKPKCVEACPAQSLSFVPWRDL
                     TDRVPPRVTNTVQILPERSASCQQCHMPSQTKNVSQGIGMFRGAVKGGRPLSAREFGF
                     RWIDLAGSVLVPLAVVGVVAHAVLRKVVNR"
     gene            complement(1407..2183)
                     /locus_tag="VEL68_10650"
     CDS             complement(1407..2183)
                     /locus_tag="VEL68_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ36467.1"
                     /translation="MKLKGQTALVTGASRGIGEAIARAFAKEGAQVAITGRSAQELKA
                     LQEEFNGLGGRCESIVADLSQKGAVDQVWRQATQAFGHIDILMNNAGIGSSANPKPVV
                     DYDDAFWEALLYVNLTVPYLFSKKALPSMIDRKYGRILMTASVNGKIGLLHGAAYAAS
                     KHGVLGLVRTLAMETAKQNITVNAICPGPVRTRMNNLRVEYEAKRLGKSVAEFEAGLT
                     PIGRRLEPHEIAPLVVYLASREASAVTGQAFNIDGGFLMV"
     gene            complement(2421..4052)
                     /gene="groL"
                     /locus_tag="VEL68_10655"
     CDS             complement(2421..4052)
                     /gene="groL"
                     /locus_tag="VEL68_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943952.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXZ36468.1"
                     /translation="MAAKEIRYDQKAREALLKGVNILADAVKVTLGPKGRNVILEKSF
                     GSPSVTKDGVTVAKEIELEDKFENMGAQMVREVASKTSDIAGDGTTTATVLAQAIYRE
                     GSKLVAAGHNPMELKRGIEKGVETVIEELKKQSKPTKEQKEISQVGKISANNDETVGN
                     IIAEAMAKVGKEGVITVEEAKSMETTLDIVEGMQFDRGYISPYFVTNPEKMEAVLEDA
                     LILINEKKISNMKDLLPVLEQIAKMGKPLLIIAEEVEGEALATLVVNKLRGTLKCAAV
                     KAPGFGDRRKAMLEDIATLTGGQMISEEMGVKLESITLKDLGKAKRITIDKDNTTIVE
                     GAGDSKGIEGRVKQIRAQIEETTSDYDREKLQERLAKLVGGVAVINVGAATESEMKEK
                     KARVEDALNATRAAVEEGIVAGGGVAYLRTLPTLEKMKLPEEQQFGVNILRRALEEPI
                     RWIAMNAGFDGSIVVEKVKNEKGNFGFDAQNEEYTDMVKAGIIDPTKVVRTALQNATS
                     VASLLLTTEAMVAERPKEKEKMPPMPPGGGMEGMY"
     gene            complement(4084..4374)
                     /gene="groES"
                     /locus_tag="VEL68_10660"
     CDS             complement(4084..4374)
                     /gene="groES"
                     /locus_tag="VEL68_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXZ36469.1"
                     /translation="MKIRPLQDRVIVKRVEEEEKTKGGIIIPDTAKEKPMEGKVIAVG
                     KGKLMEDGKVHALDVKAGDRILFGKYSGTEVKIDGDEHLIMREDDILGVIEK"
BASE COUNT          948 a         1455 c         1268 g         1256 t
ORIGIN      
        1 cgagatcaga tagaacttcg cctgagcgaa cattcccttg aagtcccgcg ccctgaatgc
       61 gtactgcctg ctcaaattcc ggctggccag gctgtacacg agccagaaga cgcaggaagc
      121 caccatcgcc cagccggcgt atcggtgcaa ccagagggcg ggatggcggg ggcccaggga
      181 tggaaccccg gagattcgga gctggatccc cgtgatgatg aggaggatga ccaacagggc
      241 gtttacccaa tgccagatcc tcaggggcag gggatggaga taggtctttt tcatcggttc
      301 accaccttcc gcagaacggc gtgggccact acaccgacca ccgccagggg aaccaaaacc
      361 gagcccgcca ggtcgatcca tcggaacccg aactctcggg cggagagagg tctgcctccc
      421 ttcaccgcgc cccggaacat cccgatgccc tggctgacgt tcttcgtttg actgggcata
      481 tggcattgct ggcaggacgc agagcgctcc ggcagaattt ggaccgtgtt cgtgaccctg
      541 ggcggaaccc ggtcggtcag gtcgcgccaa gggacgaagc tgagcgactg cgccgggcag
      601 gcctccacgc atttcggctt tccgttgcag aggtggcact tagtcgcttt gcgcgtatcg
      661 gggtcgaagg agatcatctc ccagggacag gctttcaggc acaccttgca acccgtgcat
      721 ttttccgagt cgaccatgcg cgcgttgccc gagggcgcga ggacgatggc attctccggg
      781 cagatgttgg cgcaggggac cgggtgcggg cactgcttgc agaggtcctg caccaccagc
      841 ccgtcccccc aattaccctc cccctcccgc caggccgcga ctcctccgag cccgaaattc
      901 atgttccgcc agaccttgat cctcgacatg gcgggcgcgg atttcccgtc gttgaattcg
      961 gtgcaggcca gctcgcaacg gccgcatccg acgcacttca tcgggtcccc gaccacgagg
     1021 ccctgggctt gttccataag gattaggggt ttggtggccc cttgggcgag aaccggcccg
     1081 agcaccgaga tgccgatgaa cgacaggccc ccccatttca gggtctgccg ccgggaacac
     1141 aaaggattca ggctgagcga acccagatcg gattccaaaa tctctttttt ttctacgtgc
     1201 tcttccatca ttccttgtct acctgctctt tcttacccct gatttttctc tcaaaaccct
     1261 gaaaaacaat cgtccttata atcaagggta atcggtatag tcaagaatcc atatgaccta
     1321 ggccatataa aactgccaag cccaggtttt ttcgcccggc tccacgatat gctttttggg
     1381 ggggatcaaa gcggaaaggg tttaattcat accatcaaaa aaccaccatc gatattaaag
     1441 gcctgtcccg tgaccgcgga tgcttcgcgg ctggccaggt agacgacaag cggggcaatc
     1501 tcgtggggct caagccggcg gccaatgggc gtcaagcccg cctcgaattc cgcgaccgat
     1561 ttgccaagac gtttggcttc atactccacc cgcaaattgt tcatccgcgt gcggacaggc
     1621 cccggacaga tggcgttgac cgtgatgttc tgcttcgccg tctccatggc cagcgtgcgc
     1681 accagcccca agaccccgtg cttgctggcc gcatatgccg caccgtggag taaacctatt
     1741 ttgccgttga cggatgcggt cataagaatg cgcccatact tacggtcgat catcgagggt
     1801 aaggctttct tcgagaacag gtagggcacg gtgagattga cgtaaagcaa ggcctcccag
     1861 aaggcgtcat cgtaatcgac cacgggtttg ggattcgcgc tgcttccgat gcccgcatta
     1921 ttcatgagga tgtcaatatg cccaaaggcc tgcgtcgctt gccgccacac ctgatccaca
     1981 gcgccctttt ggctgagatc ggcgacgatg ctttcgcatc gcccgcccaa cccgttaaat
     2041 tcttcctgca aagccttcag ttcctgcgcc gagcgtccgg tgatcgccac ctgggctcct
     2101 tccttggcaa aggctcgggc gatggcttct ccaatacccc ggctggctcc ggtgacgagc
     2161 gcggtttggc ctttcagttt catgcttttc ctccccagga cgattaacct aaacccctgc
     2221 cttcgggttt gctgaacgat cttatttcgg cattatccga acttcagggt gccaatgtga
     2281 gtatactgag aagacggtta tcgatcaagg aattttgaga cgggaataaa aaaagccctc
     2341 cgcttttggc agagggcttt ttccaagacc gatgataggg caaggagatt ttttagctct
     2401 atgccctacg ctctatgctt ttaatacatc ccttccattc ctcctcctgg aggcatgggg
     2461 ggcatcttct ctttttcctt gggtctttcg gccaccatgg cttcggtggt gaggagcaag
     2521 gaggcaaccg aagtggcgtt ctgcagggcc gtgcgcacca ccttggtggg gtcgatgatc
     2581 ccggccttca ccatgtcggt gtactcttca ttctgcgcat cgaacccgaa atttcccttc
     2641 tcattcttca ctttttcgac cacgatggag ccgtcgaaac cggcattcat ggcgatccag
     2701 cgaatgggtt cttccagggc tctcctaaga atgttcacgc caaactgctg ctcttcgggg
     2761 agcttcattt tttccagagt cggcagggta cgcaggtagg ccactccgcc gccagcaacg
     2821 atcccttctt ccacggccgc gcgggtcgcg ttcaacgcgt cctccacccg ggccttcttt
     2881 tccttcatct cggactcggt agcggctccc acattgatga ccgccacgcc gcccactagt
     2941 ttggccagcc gttcctgcag cttttcgcgg tcataatccg aggtggtctc ttcgatctgg
     3001 gcccggatct gtttcacccg gccttcgatg cccttggagt ctccggcccc ctcgacgatg
     3061 gtggtgttgt ccttgtcgat ggtaatccgt ttggccttgc ccaggtcctt cagagtaata
     3121 gattccagct tcacgcccat ctcttcggag atcatctgcc cgccggtcag ggtggcgatg
     3181 tcttccagca tggccttgcg ccggtcgccg aagccgggtg ctttcaccgc tgcgcacttg
     3241 agggtgcccc gcagtttgtt caccaccaaa gtggccaggg cttcgccttc cacttcctcg
     3301 gcgataatca ggaggggctt gcccatcttg gcgatctgct ccaggacggg gagcaggtct
     3361 ttcatgttgc tgatcttctt ctcgttgatg aggatcaggg cgtcctccag gacggcttcc
     3421 atcttctcgg ggttggtcac gaaatagggg gaaatgtagc cgcggtcgaa ctgcatccct
     3481 tcgacgatgt ccaaagtggt ttccatggac ttggcttcct ccacagtaat aaccccttct
     3541 tttcccacct tggccatggc ctcggcaatg atgttgccga cggtctcatc attattcgcg
     3601 gaaatctttc caacctggga gatctctttc tgctctttgg tgggcttgga ctgcttcttg
     3661 agctcttcga ttacggtttc cacgcccttt tcgatccccc tcttcagttc catggggttg
     3721 tggccggccg ccaccagctt ggaaccttca cggtagatcg cctgggccag gacggtggcc
     3781 gtggtggtcc cgtcgccggc gatatccgac gtcttggagg ccacttcgcg gaccatctgg
     3841 gcgcccatgt tctcgaactt gtcttccagc tcgatctctt tggctacggt gaccccgtcc
     3901 ttggtgacac tgggagaccc gaagctcttt tccaggatca cgttccggcc cttggggccc
     3961 agggttactt ttacggcgtc agccaggata ttgaccccct ttaggagcgc ttccctggct
     4021 ttctggtcat aacggatttc tttcgctgcc atcttttcat tcctccttta ttgattcctt
     4081 ggattacttt tcgattacgc ccaaaatgtc gtcttctctc atgatgagat gctcatcgcc
     4141 gtcaatcttc acttcggtcc cggaatactt accgaagagg atccggtctc ccgccttcac
     4201 atccagggca tggaccttcc cgtcttccat gagcttaccc ttccccacgg cgatcacttt
     4261 gccctccatg ggcttctctt tggcggtgtc ggggatgatg atcccgccct tggttttttc
     4321 ctcttcctca acccttttga caattacgcg gtcttgaagt ggcctgatct tcattctttc
     4381 cttctccttt cttcttttat gtagccggcg ctagccgatg taaaccgaat ttccttccta
     4441 aggaattgtt tttgttttat ttttttttaa ttaccccaac tctcgtatga aaagatcggg
     4501 taaaatccgc cgcggaaaaa ttcgttagca ctcattagca ttgagtgcta gtaaggataa
     4561 tataatatcc caaatttaaa ttttcaagtt tttttttaaa aaaaaattat tttttttcaa
     4621 atccagattc cctgaaatcc tatagatagt caaatgcagc ccattgattt tcaaaaccta
     4681 tccactttcg gatttatttc caagggttgg agccggaccc tggaaccatg gggtaacctt
     4741 ttatttgtct ggcttaaaaa ccaacgggga tgatgatggc agacatgtca ggattgcatt
     4801 agacctcctt ctatgctaat ttgttcgcgg acttttctta accgagggaa actttccttt
     4861 tcccaaagca tgaaaagcat tatcaggtgc atttgaattg gggaatcagt cgatgggttc
     4921 aacctga
//
LOCUS       DASPRR010000273        13753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1189303, whole genome shotgun sequence.
ACCESSION   DASPRR010000273 DASPRR010000000
VERSION     DASPRR010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189303"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VEL68_10665"
     CDS             <1..456
                     /locus_tag="VEL68_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HXZ36470.1"
                     /translation="HAVEALKRKYDFPEEVRLLDGGTLGLDLLHLIEGMDRVLFIDAV
                     DLKKSPGTVAVIEGEAIPSLLEPKLSLHHVGLADLLFASSFLGTRPAEIALVGIQPET
                     LEIGLKLSKTVMGRFEQLLQAIVKKLRGWGLEIKEKRIQEPDYVPGDSI"
     gene            434..655
                     /locus_tag="VEL68_10670"
     CDS             434..655
                     /locus_tag="VEL68_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HXZ36471.1"
                     /translation="MCLAIPSEIIEIKDKMATIDLAGVRREVSLLLLPEEVSVGEFVL
                     VHAGFAIHKIDKEAAEETLKLIEEIAEKA"
     gene            737..1102
                     /gene="hypA"
                     /locus_tag="VEL68_10675"
     CDS             737..1102
                     /gene="hypA"
                     /locus_tag="VEL68_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HXZ36472.1"
                     /translation="MHEVSLALSLLEIIEKKCREEGCQRVEAVRVRVGKASSVQPEAF
                     SFAFEVVKKDTIAENARFIIDLIPLGGTCSACGNRFETEEAYILECPSCGSPSLQITQ
                     GYELEMVEMEVNELIRPGS"
     gene            1774..2184
                     /locus_tag="VEL68_10680"
     CDS             1774..2184
                     /locus_tag="VEL68_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXZ36473.1"
                     /translation="MTRRKFLNLLLTLLGLTTVGSIVYPLGRFLAPPGEKDKSKQISL
                     KRSEIPAGEAKDIPFAGMPAMVINLPGRGFVAFSRVCSHLGCLVDYDKVRNRIFCPCH
                     AAVFDLEGNVVTGPPPRPLQKLPLRVEGESIVIG"
     gene            2189..3283
                     /locus_tag="VEL68_10685"
     CDS             2189..3283
                     /locus_tag="VEL68_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HXZ36474.1"
                     /translation="MWAKIKNWLEIRIGLTELIQTQLTEYRVPKNINLFYTLGFVALV
                     FFVLQVVTGFFLLFYYIPHSETAFRSVQEIMSTVPYGWLVRQMHVVGSTLMFVCVFLH
                     FIAVFFMGGYKKPRELTWVVGTFMFFVTIVFCLSGYLLPWSQMSYWATTIATTMPTAF
                     PYIGDMVGNLMKGSPQVSGLTLNRFFALHVTLLPLILVILIGLHLFLVRRIGISSPPF
                     GKAEEAERPWREYHHVSHPDGHPFYPDFVLKEVVMLMIFFALMFFIISFLPTLLLPEE
                     SNIAADPFKTPAHIKPEWYFLAPYQMLKIIPNKFLGISLQIILSLLFILWPFLDTKEE
                     VNILKRRRLLAFFVLGVFLWVVFTIWGKVS"
     gene            3280..4128
                     /locus_tag="VEL68_10690"
     CDS             3280..4128
                     /locus_tag="VEL68_10690"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXZ36475.1"
                     /translation="MRYILPLFILLILPSAAQADWKEQFEKEFLTRPWAGVQVQRNVC
                     IECHSSEMMKPEDRDIPRKWETSWHSQNGISCHNCHGGDPNDAENAMSPQRGYVGTPK
                     PAQIPEFCGKCHAGILKNFLESGHGKALKTSGKGPNCVTCHGSHTVQKASIQIINEPL
                     CTKCHSYGRVKEMRQALLLTENRMNEIEKSLKELKTEGVFTEEEDKSLFSTQAEFRIL
                     FHTEDVSLVKKRTDEFAHKLSSIQAKIQKTFEEIKFRKNFSAFLMMIFAGMAIGFGLL
                     AKTPKE"
     gene            4355..4813
                     /locus_tag="VEL68_10695"
     CDS             4355..4813
                     /locus_tag="VEL68_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXZ36476.1"
                     /translation="MHVLRPLFAVLAVAALILIARVFVVPDDFGIHERGYMFGWYRKA
                     NEEEWKALKVKYQGKEYCKDCHAEQEKQVLSSPHKIIECENCHGPAREHPSEPPKLTL
                     DRSRDLCLRCHTYLPYPTSKRAEIKGIDPGQHNPGAECTGCHDPHRASKP"
     gene            4880..5599
                     /locus_tag="VEL68_10700"
     CDS             4880..5599
                     /locus_tag="VEL68_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ36477.1"
                     /translation="MANGLNGIFRPLALQAATRPVRWAFLVDTYKCIGCGYCVKACKL
                     ENEIPFEADVSRTWVERYVVTKEGEVFADAPKEARYGFITSRVDLGMGKFKDIPKDRI
                     DKAFFVPKLCNQCDKPPCVQVCPVGATYQAADGVVLVDRTWCIGCGYCIMACPYGMRF
                     FHPQFHVAEKCNFCYHRINKGMRPACVLACPAGARRIGNIKDPDDPVAKVIMTQRVTI
                     HKPEYGTNPQVYYIGLSEEVR"
     gene            5603..6856
                     /gene="nrfD"
                     /locus_tag="VEL68_10705"
     CDS             5603..6856
                     /gene="nrfD"
                     /locus_tag="VEL68_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXZ36478.1"
                     /translation="MVLEEMWTAKELFTYPNEYIYWSVQIVMYPFMTGLVAGAFVLSS
                     LYHLFGIKPLKEIARFSLVFSFALLMVAPLPLLFHLLRPERNFYVFMTPHFTSAIAAF
                     GVVLFVYGSIVASELWFLYRKHLVQSSLKLKNQLNKSLAEKIGYAVYTVLTLGVFDLS
                     ESALHKDEKAIKVLAGAGVPIACFLHGYAGFIFGSVKANAMWMTPLMPVIFIMSAIVS
                     GVALCMLTYIITMEIRKALASRKRASFVYPTFEEIKGVEVYEVKMTAKYLLLFLIFSI
                     SLELLDLVFRGYTAVRSWDILRLVIYGKDFTNIFILQYTLGNLIPFILLLIPGLTIRR
                     AVFALLLVIFGVFMMRWNVVIGGQSFSLTFSGYMDYLPPLIPRDLATFKEGLTGALLI
                     LGLPFVLFWVLSRVFPVFDLKESPR"
     gene            6905..8434
                     /locus_tag="VEL68_10710"
     CDS             6905..8434
                     /locus_tag="VEL68_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ36479.1"
                     /translation="MFRSLFLKVSLSVGLVITLTLASFAYFLMENQKEHLLDTKKKEI
                     EILSTLVSNWIANFMLEGRAQDFHNFFGPFRVREEQLELRILDPSGAVLLSSQRNEKG
                     ISMAYLLAGHPDLSGKTPMVREQWIQGRPFFSSLQALENQPSCFSCHGEDKKILGLLN
                     VALPMEMTHRSIAFNRNLLIASTAITLLLMAAAINFLLTRLVKKPIALLIETMSQVEK
                     GNLNVEVNLGTRDELGHLGRSFSSMVQKLSLAQKELEKQQQQQMLQVKHLASLGELAA
                     SVAHEVKNPLAGIKLAIQVLAKEPCVADSQRETIEEIVRSIDRLDKTITDLLSYSRIS
                     PPALSPVNLHEVIEAALFSVKEEAHIAGVRVEKLFDLTLPLLPLDSRQMEGVFLNLFL
                     NALQAMPQGGVLTVQTKRRESGHPRQENPPGSPGSEEGWVEVTVTDTGEGIPPEVLGE
                     VFRPFFTTKAKGTGLGLSLVQRVVEQHHGQVFVQSGTGVGTTFYLLLPISPQPPKGEP
                     V"
     gene            8431..9801
                     /locus_tag="VEL68_10715"
     CDS             8431..9801
                     /locus_tag="VEL68_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ36480.1"
                     /translation="MKEKILVVDDERLIRQSLEKGLSGQGYAIFSAGDGKTALRLAEE
                     ESPDLIVLDLKLPDLNGIEVLKSLRELEKDMPVLVITAYGSIDTAIQAIRSGAYDYIT
                     KPFDLEALLLSISQALEASRLKKEVTYFRSQETTRSGLHRIIGQSPAMKEVGRLVRQI
                     AQSEASTVLLEGESGTGKDLVARTIHTESARVNQPFIEVNCAALPETLIESELFGHER
                     GAFTDAKTRKAGLLETANRGTLYLDEIGDMNPPLQSKLLSMIENKRFRRLGGVKDLSV
                     DVRFIAATNRDLASAVEKGGFRKDLYYRLKVFSIQLPPLRERLSDLPQLIGFFLAELQ
                     REFKKKIVGLSPEAEKVLADYHWPGNVRELRNVLERAFILCEEDRIEPSHLPPELTAP
                     ITPPPKAGESLFRLPPEGVHLETLEEDLIRQALEVSSGNQVRAAKLLGISRDALRYRM
                     KKFNWT"
     gene            10029..10943
                     /gene="nrfD"
                     /locus_tag="VEL68_10720"
     CDS             10029..10943
                     /gene="nrfD"
                     /locus_tag="VEL68_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXZ36481.1"
                     /translation="MNDILITYDVHHAAPFGPLIVLFIAITGLNAGAYLASVVFAHLD
                     KKEYLPLAKLSALAVILLWAAAPLLLLLDIGQPLLFWHLFAYFQPESPMAWGTLILTI
                     YPFLGSVYIWYLFRGEIQKAKVWGLIGLPIALGSHGFVGFVLSFSTARILWTTSVTPI
                     FFLVSAALSGLALVVILDTVRYYSTLRHSPEAQARERLIFHHLGEGLYILIFADLSLI
                     LFYLMKLGLTPELFDHVLKLMTEGKLSIADLFIPLVLGLMAPLALLVAPRTARNPVSQ
                     LIASALIIFGVFFMGNLILSAAQALPLV"
     gene            complement(11031..11915)
                     /locus_tag="VEL68_10725"
     CDS             complement(11031..11915)
                     /locus_tag="VEL68_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ36482.1"
                     /translation="MAYDYKLVRGEKGKGVLKITLNQPDTLNALTPDLERELHAALQE
                     GDNDPEVFCMVICGAGRAFSSGYAMGAVPGRKGSLTDPAIYGSIGEFLGIVQVHDYEN
                     VQRRQLDLWRLKKPVIAAVHGYCMGGGMWLAMACDMCYCSEDTVFGQPEVRHNANSTF
                     LIPALAGWHRAARYLYTGDHFDGKEAERIGLVNECLPKEKLMPTVMALAERIALVPPN
                     SVRIMKRLILSGYMAYGIGAAMEVCAPYSTLAHCSHGPERQAMFDAQKERGMKGFLEE
                     RDGKFLPEPFGPKSKVKK"
     gene            12370..>13753
                     /locus_tag="VEL68_10730"
     CDS             12370..>13753
                     /locus_tag="VEL68_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36483.1"
                     /translation="MKGSTRWYLLWIMAICLSVALTGPGLGGVAYAKDKLVVWWNKGY
                     YKEEDEGMQKIAAEFAKQNNVDVELTFTIQDDLGPKIISALIARRVPDVAFCFFTDWQ
                     IAPKFAWEGKLADVTDLINQLKPRYLESHLKTGWLYNNVAKKWSYYSVPIEAQALGTH
                     YWKDLVQEAGLDPDPKKIPMKWEEHWAFWKKVQDVLRKKDPAKYGKLFGIGMTESSRS
                     TDTFYNFEQYLLAYRGEVISPEGKVVAAEPKNRDAIVKTLAFFTQIYSEGYVPPDALT
                     WTDADNNVNFNNKTVVMTPNPSISIPGAHFFTNKDNYYNKIGSILQPTSPVDGKGYPY
                     LVSVKPIIIPMDSKQHALAKKFISYVLETKNFTSYVTASNGRWFPSFKDVAADPFFTN
                     PKDPHVPVATDQHLNHETRPFYYSYHPAYSQVYTENVWGKAMSRVVTEKWTNEKAADE
                     AIDRIQTIFKS"
BASE COUNT         3102 a         3841 c         3549 g         3261 t
ORIGIN      
        1 cacgccgtgg aggctctcaa aagaaaatat gatttccccg aagaagtgcg cctcctggac
       61 ggaggaaccc tggggctcga cctcctccac ctgatcgaag gaatggatag agttctgttc
      121 atagatgcgg tagacttaaa aaagagtccg ggtaccgtcg cggtcatcga gggggaggcc
      181 attccttctc tcctagaacc caagctctcg ttgcatcacg tcggcctcgc ggaccttctc
      241 tttgcgtcca gttttctggg aactcggccg gcggaaatcg ccctcgtggg tatccagccc
      301 gagaccctgg aaatcgggct gaaattatcc aagacggtca tgggccgatt tgaacaattg
      361 ttgcaggcta ttgttaaaaa actccgggga tggggtttgg aaatcaagga aaagagaatt
      421 caggagcccg attatgtgcc tggcgattcc atctgagatt atagagatca aggacaagat
      481 ggccactata gaccttgcgg gagtccgccg ggaggtgagt ctgctccttc tcccagaaga
      541 ggtctccgta ggagaattcg tcttggtcca tgccggcttc gccatccata agattgacaa
      601 ggaagcggcc gaagaaaccc tgaaattgat agaggaaatt gccgaaaaag catagcgcag
      661 agcgcataga gcagagcgtt cagtatttca cgccatgccc tatgcgctgt gcgtcttctc
      721 gtgaagcgat acgtttatgc acgaagtttc cctggccctt agccttctgg agatcatcga
      781 gaagaagtgc cgggaggaag ggtgtcaaag ggtcgaagcg gtacgggtcc gagtgggaaa
      841 ggcctcgagc gttcagcccg aggctttttc ttttgccttc gaggtcgtta aaaaggatac
      901 aatagccgag aatgcgcgat ttatcatcga tctaatccct ctcggtggaa cctgctctgc
      961 ctgcgggaac cgtttcgaga cggaagaagc ctatatcctg gaatgccctt cctgcggctc
     1021 cccttccctt cagatcaccc aggggtacga actggaaatg gtggagatgg aggtaaacga
     1081 actcatccgt cctggctcat gaattccccg ccccatttgc ggggacaggg aaaggctgga
     1141 gcgactgtcc gccgcccctc gaccggccat tgcgcgaggg aattgggagt aagccggtgt
     1201 atgccgtggg cggaaagtta tttgaggatg cccgcctccc gtgtggattg atcttttttc
     1261 accctcgctc actacttatt tccagcttct acttatctat ccggcgcgat cggaacaaag
     1321 ctttctctgg agaattttct cgcccgcctg cccgcccgcc tgcaaaaaaa cttggtgttt
     1381 cacagccata ggcatggaac gccataaaat attttccata aaatattttc ttgacagtat
     1441 ctatcacatt gtggtactcg atcttttttg gccgaattta tttcccggtg attgaccggt
     1501 cgggagattg agagaggaat tctctcctgc cagactgctg gaggggataa atcatggcgc
     1561 atggactcct acagtatcta ttcccaagaa atactgtctt caccccattc tttttgcaat
     1621 ctcttgcagc agaataataa ttaggggcta tttacggatg tcacttttgg gttttatcct
     1681 caatgctacg gttcaagaga gacaagattc aatggcgaaa ggggaaataa taatgttttt
     1741 gcttcccaac gcgggaccag aaagctaaat atcatgacga ggagaaaatt tttaaacctt
     1801 ttgctgaccc tgctcggatt gactactgtc ggttccattg tttacccctt gggaagattc
     1861 cttgcgcccc ctggagaaaa ggacaaatcg aagcagatca gcctcaagag gtctgaaatc
     1921 cccgcggggg aagcaaagga tattcccttt gcgggtatgc cggcgatggt tatcaacctg
     1981 ccgggcaggg gattcgtcgc cttctcccgc gtctgttccc acctgggctg tctggtggat
     2041 tatgataaag ttcgaaaccg aatcttttgc ccttgtcatg ctgccgtatt cgatctcgaa
     2101 ggaaacgtcg taacgggccc cccgccgagg cccttgcaga agctccccct cagagtggaa
     2161 ggggaatcca ttgtgattgg ataacgagat gtgggcgaaa attaagaatt ggctggaaat
     2221 tcggattggg cttacggaac tcatccaaac acagcttacc gagtaccggg tgccaaagaa
     2281 tataaatctt ttttacaccc tcgggtttgt cgccctggtt ttctttgtgc ttcaggtagt
     2341 caccggcttc ttcctcctct tttattacat tccccattcg gagacggcct tccggagcgt
     2401 tcaggaaatc atgagtacgg tcccctatgg ctggctggtc aggcagatgc atgtagtggg
     2461 aagcaccctc atgttcgttt gtgtttttct gcattttata gcggttttct tcatgggcgg
     2521 ctataaaaag cccagagaac ttacctgggt tgtgggaact ttcatgttct ttgtcacgat
     2581 cgtcttttgc ctcagcggtt atctccttcc gtggagccag atgagctact gggccacaac
     2641 cattgccacg accatgccca cggcgtttcc ctacatcggc gatatggtgg gcaatttgat
     2701 gaaagggagt ccacaggtct ccggcctgac cctcaacagg ttcttcgctc tccacgtgac
     2761 gcttctaccc cttattctgg taatcctgat cggcctccat cttttcctgg taagacgcat
     2821 cggcatctcc tctccgccct ttgggaaagc ggaggaagca gaaaggccat ggagggaata
     2881 tcatcatgta agtcacccgg atgggcatcc cttctacccg gatttcgtat taaaggaagt
     2941 ggtcatgctg atgattttct ttgccttgat gttctttatt atcagcttcc tgccgaccct
     3001 tctcttacct gaggagtcca atatcgctgc cgatcccttc aagacccccg cccatataaa
     3061 accggagtgg tatttcctgg ccccttacca gatgcttaaa atcatcccca acaaattcct
     3121 gggaatcagc ctgcaaatca tcctttcgct cctatttatc ttgtggcctt tcctggatac
     3181 caaagaagaa gtgaacatcc tgaaaaggcg tcgtttgctt gcgtttttcg tcttgggtgt
     3241 atttttatgg gtcgtgttta cgatatgggg aaaggtttca tgagatacat actcccatta
     3301 ttcattctcc tgattcttcc ctccgccgcc caggcggact ggaaggagca atttgaaaag
     3361 gaatttttaa cccggccctg ggccggggtc caggtccaaa ggaatgtctg catagaatgc
     3421 cattcctccg aaatgatgaa acccgaagac cgggacattc cccggaaatg ggaaaccagc
     3481 tggcattccc agaacggaat ttcttgccat aactgccacg ggggtgaccc caacgatgct
     3541 gaaaatgcca tgtccccgca aagaggttat gtgggaaccc ccaaacctgc ccagattcct
     3601 gaattctgcg ggaaatgcca cgccggaatt ctaaaaaatt tcctggagag cggccatgga
     3661 aaagccctga aaacctcggg aaaaggacct aattgcgtca cctgccacgg gtcccacacc
     3721 gtccagaagg cgagcatcca gattatcaat gagcccctgt gcaccaaatg ccattcttac
     3781 ggacgggtca aagagatgag acaggccctt ttgttgacgg aaaacaggat gaacgaaatc
     3841 gagaaaagcc taaaagagtt aaaaaccgaa ggggtattca ccgaggaaga ggataagagt
     3901 ctcttcagca cccaggcgga attccgcatt ctcttccata ccgaagatgt atctctggtt
     3961 aaaaagagaa cggatgaatt cgcccataaa ttgagctcga ttcaggctaa gatccaaaag
     4021 acctttgaag aaatcaagtt caggaagaat ttctcggctt ttttaatgat gatcttcgcc
     4081 gggatggcga tcggatttgg gctgttagcc aagactccta aagaatagaa ggaagaaacc
     4141 tatccattaa cttgacgttg tataccattc tgtggtaagc aaccgtgatt ttctcggttc
     4201 ctgcaccgga atcaggatca taaagccata ccgatcgggc ggaatcgaga ccggagttgg
     4261 cagcctggag ttttaagccc ttttcctatc ccgatgcttc atccgggaag gaaaaggttt
     4321 tttgttgtca ttttcccaat aaggttatga gctcatgcat gttctgcgcc ctttgtttgc
     4381 agttttggct gtagccgctc tgatcctgat cgccagggtc ttcgtcgttc ccgatgattt
     4441 cggcatccat gaaagggggt acatgttcgg ctggtatcgg aaggcgaatg aagaggagtg
     4501 gaaagccctc aaagtcaaat atcaggggaa ggaatattgc aaggactgcc atgccgaaca
     4561 ggaaaagcag gtactctcat ccccccacaa aataatcgag tgtgagaact gccatggacc
     4621 ggcccgggag catccgtcag agcccccgaa acttaccctc gaccggagcc gcgacctctg
     4681 cctcaggtgc catacttatc ttccctatcc cacgagcaag agagcggaaa tcaagggcat
     4741 tgaccccggt cagcataatc ccggtgcaga atgcacgggc tgtcatgatc cccatagggc
     4801 atccaagcct taacaggagg agcagttgaa gaggagggat tttttgaagt caatcctgaa
     4861 agggtccgcc gccgtggccc tggccaacgg gctgaacggc atttttcggc ctttagccct
     4921 tcaggccgcg acgaggcccg tgcgctgggc ctttctggta gacacctaca agtgcatcgg
     4981 ctgcggctac tgcgtcaaag cctgtaagct cgaaaatgaa atccctttcg aggccgacgt
     5041 ctcccggacc tgggttgagc gatacgtcgt taccaaagaa ggagaggtct ttgcggatgc
     5101 ccccaaagaa gcccggtacg gattcattac cagccgggtt gatctcggaa tgggaaagtt
     5161 caaggatatt cccaaggaca gaattgacaa ggccttcttt gtgcccaagc tttgcaacca
     5221 atgcgataaa ccaccctgtg tccaggtgtg tccggtagga gctacctatc aggccgccga
     5281 cggggtggtc ctggtggacc gcacgtggtg cattggctgt ggctattgca tcatggcctg
     5341 cccttacggg atgaggtttt tccaccccca attccatgtg gcggagaagt gcaatttctg
     5401 ctatcaccgg atcaataaag gaatgaggcc ggcctgcgtt ttggcctgcc cggcgggcgc
     5461 gaggagaatc ggaaatatca aggacccgga cgatccagta gccaaggtca tcatgaccca
     5521 gcgggtaacc attcacaagc cggaatatgg gaccaatccc caagtttatt atatcggcct
     5581 cagtgaggag gtaaggtaag gcatggtcct ggaagaaatg tggacggcaa aggaactctt
     5641 cacttacccc aatgaataca tttattggag cgttcagatt gtcatgtacc ctttcatgac
     5701 cggcttggtg gccggcgcct ttgtcctttc ctctctgtac catctcttcg gcataaaacc
     5761 gctcaaggaa atcgcccgat tttccctggt cttctccttt gccctcttga tggttgcccc
     5821 cctgcccctg ctctttcatc tccttcgccc tgaacggaat ttctatgtat tcatgacccc
     5881 ccacttcacc tcggccatcg ccgcttttgg ggtcgtcctt ttcgtctacg gctccatcgt
     5941 cgcttccgag ctgtggttcc tctaccggaa acacctggta caatcttccc taaagctcaa
     6001 aaaccagttg aacaaaagcc tggccgaaaa aatcgggtat gcggtctata ccgtcctcac
     6061 cctgggggtc tttgacctca gcgaatccgc cctccacaag gatgaaaagg ccatcaaggt
     6121 gctggctggc gcgggagttc ccatcgcctg ttttctccac gggtatgccg gttttatatt
     6181 cgggtcggtg aaagctaacg ccatgtggat gacccccctc atgccggtca ttttcatcat
     6241 gtccgcgata gtctccggtg tggccctctg catgctcacg tatatcatca ccatggaaat
     6301 ccgaaaggcc ctcgcctccc gcaaaagggc ttccttcgtt tacccgacct ttgaagagat
     6361 caagggcgtc gaggtctatg aggtaaagat gacggccaaa tacctcctct tgttcctgat
     6421 cttttccatc agcctggaac tactggacct ggtcttccgg ggatacacgg cggtaagatc
     6481 ctgggacatc ctgcggctgg tgatctatgg aaaagacttc accaatatct tcatcctcca
     6541 gtataccctc gggaatctca tcccttttat cctcctcctt atcccgggcc tcaccatccg
     6601 gagggccgtc ttcgccctcc ttctggtcat ttttggcgtt ttcatgatgc ggtggaatgt
     6661 ggtcatcggg gggcagtcct tctcccttac tttttccggg tacatggatt accttccgcc
     6721 cctgatcccc cgggatttgg cgaccttcaa ggaaggcctg acgggcgcgc tcctcatcct
     6781 gggattgcct ttcgttctgt tctgggtgct cagccgggtc tttcccgttt ttgacctgaa
     6841 agagtcccct cgataggagg gaaggagagc ccctctgcat cttccccggc acggtgaacc
     6901 gctcttgttt cgcagcctct tcctgaaggt gagtctctcc gtggggttgg tcattaccct
     6961 taccctggcc tcttttgcct acttcctaat ggaaaatcag aaggaacatc tcctcgacac
     7021 caagaagaag gagatcgaaa tcctctccac cctggtttcg aattggatcg caaacttcat
     7081 gctggaagga agggcccagg actttcataa tttcttcggc cccttccggg tacgggaaga
     7141 acagctggag ttgcgcatcc tagacccctc cggagccgtc ctcctctcct cccaaaggaa
     7201 cgaaaagggg atttccatgg cttatctgct cgccgggcac ccagacctct cggggaaaac
     7261 cccgatggtc cgcgagcaat ggatccaggg gcggcccttc ttcagctccc tccaggccct
     7321 cgagaatcag cccagctgct tttcctgtca tggggaggac aaaaaaatcc tgggcctgct
     7381 gaacgtggct ttacccatgg agatgaccca tcggtctatc gctttcaacc gcaatctgct
     7441 catcgcctca acggccatta cccttctgct gatggccgca gcgatcaatt ttctcctcac
     7501 ccggctggtc aaaaagccga tcgctcttct catcgagacc atgtcccagg tggagaaagg
     7561 aaatctcaac gtggaggtga acctgggcac tcgggacgaa ctgggacacc tgggccgaag
     7621 cttcagctcc atggtccaaa aactttccct ggcccagaag gaactggaaa agcaacagca
     7681 gcagcagatg ctccaggtca agcacctggc ttccctgggg gagttggccg cgagtgtggc
     7741 ccacgaggtg aagaatcccc tggccgggat caagctggcc atccaggtgt tggccaagga
     7801 accctgcgtg gcggacagcc agcgagaaac cattgaggag atcgtccgtt ccatcgaccg
     7861 cctggataag accatcaccg acctcctctc ctattcccgc atttccccgc ccgccttgag
     7921 ccccgtcaat ctgcacgaag tgattgaggc ggctcttttc tcggtcaaag aagaggccca
     7981 cattgccggc gtccgggtag aaaagctttt tgacctgact cttcccctct tgcccctgga
     8041 ctcccggcag atggagggcg tcttcctaaa cctcttcctg aatgctttgc aggccatgcc
     8101 ccagggagga gtcttaaccg tccagacgaa acgacgggaa tccgggcatc cccgccagga
     8161 aaatccaccc ggaagccccg gctcggaaga aggttgggtt gaagtcacgg tgacggacac
     8221 gggagaagga attccacccg aggtgctggg cgaggtcttc cggccttttt tcaccaccaa
     8281 agcaaaaggc acgggcctag gcctttcact ggttcaacgg gttgtggaac agcatcacgg
     8341 gcaggttttt gtccagagcg ggacaggcgt gggaaccaca ttttatctgc ttctccccat
     8401 ttcgccccag ccgcccaaag gagagcctgt atgaaagaga agatcctggt cgtggatgat
     8461 gaaaggctga tccgccagtc tttggaaaaa gggctctccg gccagggcta tgcgatattc
     8521 tccgcaggag atgggaaaac cgcccttcgc ctggcggaag aagaatctcc agacttgatt
     8581 gtgctggacc tcaagctgcc ggacttaaat gggattgaag ttctcaagag cctgcgcgaa
     8641 ctggaaaaag atatgccagt tttggtcatt acggcctatg gcagcatcga tacggctatt
     8701 caagccatcc gctcgggagc ttacgattac ataaccaagc ccttcgacct ggaagccctc
     8761 ctcctttcca taagccaggc tctggaggca tcccgtctga aaaaagaggt cacttatttt
     8821 cggtctcagg agacgacccg gtctggtctc catcggatca tcggccaaag tccggcgatg
     8881 aaggaagtgg gccgcttggt caggcagatc gcccagagcg aagcatccac agtcttactg
     8941 gaaggggaaa gcgggacggg caaggatttg gtggcccgca ccatccatac cgagagcgca
     9001 cgggtgaacc aaccgttcat cgaggtcaac tgcgccgccc ttcccgagac gctcatcgag
     9061 agtgaactct tcggccacga gagaggggcc ttcaccgatg caaaaactcg gaaggccggc
     9121 ctcctcgaga ccgccaaccg gggcaccctg tatctggacg agatcggaga catgaatccc
     9181 cccctgcagt ccaagctgct cagtatgatc gagaacaaaa ggttccgcag gctgggcggg
     9241 gtgaaggacc tgtccgtaga tgtgcggttc atcgcggcta cgaatcggga cctggcttcg
     9301 gcggtggaaa aggggggttt tcggaaagac ctctattatc ggctcaaagt cttttccatt
     9361 cagcttcccc cgcttcggga aaggttgtca gaccttcctc agctcatagg ctttttcctt
     9421 gctgagctcc aacgggaatt caaaaagaag atcgtggggc tctctccgga ggcggaaaaa
     9481 gttttggccg actaccactg gccgggaaat gtgcgggaat tgagaaatgt tctggagcgg
     9541 gccttcattc tctgcgagga ggaccggatc gagccctccc acctgcctcc tgaactgacc
     9601 gctccgatca cccctcctcc caaagcggga gaatctctct tccgccttcc cccggagggg
     9661 gtccatctgg aaacccttga ggaggatttg atccgccagg ccctggaagt ctcctcgggg
     9721 aaccaggtgc gggcagccaa gcttctggga atcagccggg atgcccttcg ctaccgcatg
     9781 aagaaattca actggacctg agaatgggtg atttccccca cccctgggtc atttcaccca
     9841 ggggcatcca ttcggatctt gcctttcctc ccgggagttg atcgtcttcc cttttcctta
     9901 agcagggtta agccttcaaa aagaattgaa atttcggagg tccgccggaa aagccaggca
     9961 gtgctggagg tccgtttcca ggctggcatg aggattgctc ttcaaaatga atcaggaggg
    10021 attcaaccat gaacgacatt ctaataacct acgatgtaca tcacgcggcg ccattcggtc
    10081 ccctgatcgt cctgttcatc gctataaccg gcctgaacgc gggagcctat ctggcgtcgg
    10141 tggtttttgc ccacctggat aaaaaggaat acctaccctt ggcaaaatta tcggccttgg
    10201 cggtcattct tctgtgggcg gcggctcctc tccttcttct tctggacatc ggccagcctc
    10261 tcctcttctg gcatctcttt gcctattttc aacccgagtc ccccatggct tggggcacgc
    10321 tgattcttac gatctatccc tttctgggtt cggtgtacat ctggtatctc ttccgcggtg
    10381 aaatccagaa agccaaggtt tggggactta tcggcctgcc cattgcgttg ggatcgcatg
    10441 ggtttgtggg cttcgtgttg agtttttcca cagcccgcat cctctggacc acttcggtca
    10501 ccccgatctt tttcctggtc agcgccgccc tctcgggctt ggctctggtc gtaatcctgg
    10561 acacggtgcg gtattattcc accctacggc attcgcccga agctcaggcc agggagcggc
    10621 taatcttcca tcacctgggc gaaggcctat acatcttgat ctttgccgac ctgtccctga
    10681 tcctcttcta cctcatgaaa ctgggcctga cccccgagct ctttgatcac gtccttaaac
    10741 tgatgaccga aggaaaactc tcgatcgccg acctcttcat tcccctggtc ttggggctta
    10801 tggctcccct ggccttgttg gtcgcaccca ggacggcccg caatccggtc agtcagctga
    10861 ttgcttcggc tttgatcatc ttcggggtct ttttcatggg gaacttgatt ctttccgctg
    10921 cccaagccct acccctagtt tagttgttct atgttgtggg cggccacgga gggccgcccc
    10981 tacaaatact gttgttaacc gcgttcatct gtgaaaatct gcctcccatt ctattttttg
    11041 acctttgatt tcggtccgaa gggctcaggc agaaacttcc catccctctc ctccaggaag
    11101 cccttcatcc ccctctcttt ctgcgcatcg aacatggcct ggcgttccgg accatgggag
    11161 cagtgggcca gcgtggaata gggggcgcaa acctccatag ccgccccgat cccataggcc
    11221 atgtagccgg agaggatcag ccgcttcatg atgcgtaccg aattgggtgg aaccagggcg
    11281 atcctttcag ccagggccat gaccgtgggc atgagcttct ccttgggaag gcactcgttc
    11341 accagcccga tccgctccgc ttctttccca tcgaaatggt cccccgtata taagtaacgg
    11401 gcggcccggt gccagccggc cagagccggg atcaagaaag tgctattagc gttgtgtcgg
    11461 acttccggtt ggccgaacac cgtgtcttca gagcagtagc acatatcgca ggccatggcc
    11521 agccacatgc ccccacccat gcagtagccg tgaacggcgg caatcaccgg tttcttcagc
    11581 cgccacaggt ccagctggcg gcgctgcaca ttctcgtagt cgtggacctg gacgatcccc
    11641 agaaattcgc cgatgcttcc gtagatggcc ggatcggtca ggctcccctt tctcccggga
    11701 accgctccca tggcatagcc ggaggaaaaa gcccggcctg cgccgcagat caccatgcag
    11761 aaaacttcag gatcgttgtc tccttcctga agggcggcat gaagttcccg ttccaggtct
    11821 ggagtcagag cattgagagt atcgggctgg ttcagggtga tcttcaagac ccccttaccc
    11881 ttttccccgc gaaccaattt gtagtcgtaa gccatagtgc accccccttt cattgcggat
    11941 ttctcatgga acaatgctat ttcagattaa agaccgcagg catgatggcc ccaaagaccc
    12001 cggtcacccc gacctgtacc tggccgtgct aatcaccgga atttgaagcg ggttcataat
    12061 ccctctccgc aagaatggaa aatccaatgc tgaaaaacca ggaagcatgt ccttgtttgg
    12121 tgaaatcatt ggccattatt tctctcctgt caataaaatt tattgacagg gtctctccta
    12181 ccttcgtaca ataccttgcg taatatgagg ccaactctct ccaatctccc ttggctaaag
    12241 gaggaggcgg gaggatggat ggtgctctta gccccaagga aatattattt ttcagggtgc
    12301 agctttgctg cacgggattc ttggccgtta ggtctttacc attcctggaa tgatgaaagg
    12361 aggaagaaga tgaaaggaag tacgcggtgg tatttactct ggattatggc catctgcttg
    12421 tccgtggctc tcaccgggcc gggattgggt ggcgtggcct acgcaaagga caaactggtc
    12481 gtctggtgga ataaggggta ctacaaggaa gaggacgaag gcatgcagaa aattgccgcc
    12541 gagttcgcca aacagaataa tgtggatgtg gaattgacct tcaccatcca ggacgacctc
    12601 ggccccaaga tcatcagtgc cctgatcgcc cgtcgggttc ccgatgtggc cttttgtttc
    12661 ttcaccgact ggcagattgc gcccaagttt gcctgggaag gaaagctggc cgacgtcacc
    12721 gatctgatca atcagttaaa accccgctat cttgagtccc acctcaagac cggctggctc
    12781 tataacaacg tggccaagaa gtggagctac tactccgtac ccatcgaggc gcaggctttg
    12841 gggacccatt actggaagga cctggtgcaa gaagcaggtc tggaccctga tcccaagaag
    12901 attcccatga agtgggagga gcactgggcc ttctggaaga aagtacagga cgtcctgcgc
    12961 aagaaggacc cggccaagta tggaaaactt ttcggcatcg gcatgaccga gtcaagcagg
    13021 tccaccgaca ctttttataa cttcgaacag tacctcctgg cctaccgggg cgaggtcatt
    13081 tctcccgagg gtaaagtcgt agctgcggaa cccaagaacc gggacgccat tgtcaagacc
    13141 ctggctttct tcacccaaat ctacagcgaa ggttacgttc ctccggatgc tctgacctgg
    13201 accgatgcgg acaataacgt caacttcaac aacaagacgg tggtgatgac ccccaacccg
    13261 tctatttcca tcccgggcgc ccatttcttc acgaacaaag ataactacta caacaagatc
    13321 gggtccatcc tccagcccac ttccccggtg gacggaaagg ggtatcccta cctggtatcg
    13381 gtgaaaccga tcattatccc catggattcc aagcaacacg ctctggccaa gaagttcatc
    13441 tcgtatgtac tggagaccaa gaacttcacc tcctatgtga ccgcttccaa tggccgctgg
    13501 ttcccatcct ttaaggacgt tgccgcggac cccttcttca ccaatcctaa agatccccat
    13561 gtgcccgtcg ccacagacca gcacctaaac catgagaccc gtcctttcta ctacagttac
    13621 cacccggcct actcccaggt gtacaccgaa aatgtctggg gaaaggccat gtctcgggta
    13681 gtgaccgaaa agtggaccaa cgaaaaggcc gcggatgaag ccatagaccg catccagacg
    13741 attttcaaga gct
//
LOCUS       DASPRR010000274         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1839046, whole genome shotgun sequence.
ACCESSION   DASPRR010000274 DASPRR010000000
VERSION     DASPRR010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839046"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VEL68_10735"
     CDS             complement(<1..254)
                     /locus_tag="VEL68_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36484.1"
                     /translation="MTTEFWKWAKDIWRRVPKTAVIVLLLLVVTGISADLLALHDPEI
                     GDPRQRLLPPAWESGGKWTYPLGTDTMGRDVLSRLIYGSRV"
     gene            complement(279..1196)
                     /locus_tag="VEL68_10740"
     CDS             complement(279..1196)
                     /locus_tag="VEL68_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36485.1"
                     /translation="MKRYIIRRFFFGIIVVWIVSVMIFLATRIGPDPALMIASPGADE
                     TELNSIRDRFGLNEPLPVQYFIFVKKALRFDFGESIYYGMPVSEIVWQRLPATLILVG
                     TAKFISLLFGVPAGILAARRRGSLTNNFLRWFSFLGLSMPNFWIAMLFILIFSVKLKV
                     LPTGGYGTFWHLLMPAFSLGWYFSAGYTRITESSLLQVLNSEYIKLCRVKGLTEFSVV
                     AKHALKNSLIPVVTLAGMNIVIMVSSAVAIEMVFAWPGLGLLMYHGAIYRDFNVVQAV
                     ILFISVSMVFINLAVDILYAYLDPRIRYD"
     gene            complement(1522..3060)
                     /locus_tag="VEL68_10745"
     CDS             complement(1522..3060)
                     /locus_tag="VEL68_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36486.1"
                     /translation="METGKKKRLEKITFFMLFLLCSSATQVWAAPPAGVLKQAIHWSF
                     SADWLDPAFGTFPIPAYHPMYLFHDALFKPMAQGLYTPSLAESWTVSPDARVYEFKLR
                     QGVRFHNGDPLTAEDVVFSFMRYKGSLAKAIHGKTEKAEAVNPYSVRVRFKESFPDFL
                     EYLLPGSTTFGWIAPKKYLEKVGDAGFKKHPIGAGPYKFVEHVPGVKLVGEAFEEYWR
                     KVPHVKRLEFYNIPEPATRLAMVKRGEADIGTLMQGVYYEDLKKSQGLRMLAPLSPVR
                     WVAYLTAQWDPKSPWADPRVRMAASLAIDRQTLADVHMPGCGGVSNIALEGDPMAVDF
                     PPYPYDPEKAKKLLAEAGYPQGFQAGKFYPYEGGYWPYGEQIATYWKAVGISVETVLL
                     DKPALLAMRQGGKMKGGIFIDNPTSPTIGGRLSTMFSGGSYGNYPDIQALWDQYGKET
                     SSQGRRDLMARIQKMIYERTMWIPLTSTNSPVAIGPRVKGNPYKIQPLIWFTAPFEDI
                     EIVN"
     gene            complement(3134..>3843)
                     /locus_tag="VEL68_10750"
     CDS             complement(3134..>3843)
                     /locus_tag="VEL68_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXZ36487.1"
                     /translation="VQVADVAMERAFRRIESGVRECEVLATAMETLYSFGMEVPQCSL
                     IVASGENTAPLHRFASDKLILRGDLVFLDLGGCFNGYFSDFTRTVCLGRPSEQQKKIY
                     TAVYETMMNIVETMKPGVTNEFVNRSARGIIQKHGFEKFGFHGLLGHSIGTSGLTYPI
                     IGELAATGEKVFELQPGMIFSLEPTITVPGVPGGGGVRIENNILITEKGNEVLNKTPY
                     CQKMLGKSSCDGPCCTR"
BASE COUNT          856 a         1130 c         1026 g          831 t
ORIGIN      
        1 acccttgagc cgtaaatcag cctgctcagc acatcccgcc ccatcgtgtc ggtccccagg
       61 ggataggtcc atttcccccc gctctcccag gcgggcggca gcaaccgctg ccgggggtcg
      121 ccgatctccg ggtcatggag cgccaggagg tcggcggaga ttccggtcac cacgaggagg
      181 aggagaacga tcaccgccgt tttcggaacc cttctccaga tatccttcgc ccatttccaa
      241 aattctgtag tcatcgccgt tcttccgttt tccctgtttc agtcataacg gatgcgtggg
      301 tccaggtagg cgtaaagaat atccacggcc agattaatga agaccatact taccgagata
      361 aacaggatga ccgcctggac tacattaaaa tcgcggtaga tggctccatg gtacatgagg
      421 agtcccaatc ccggccaggc aaagaccatc tcgatggcca cggcgctcga gaccatgatc
      481 acgatattca tgcccgccag ggtcaccacg ggaatcagcg aatttttcaa cgcgtgcttg
      541 gccacgaccg agaactcggt aagtcccttc acccggcaga gcttgatata ttcgctgttc
      601 aaaacctgca gcagagacga ctccgtgatc cgggtatatc cggcactaaa ataccagccc
      661 aaggaaaacg ccggcatcag gagatgccag aaggttccat acccgccggt gggtaaaacc
      721 ttcaatttca cggaaaaaat taggatgaac agcatggcaa tccaaaagtt gggcatggac
      781 aggccaagga acgaaaacca tcgcagaaaa ttgttagtca aactgccgcg ccttctggcc
      841 gccagtatac ccgccgggac tccaaagagc agcgatatga acttcgcagt ccccaccagg
      901 atcaacgtag ccggcagcct ctgccagacg atctcggaaa ccggcatgcc gtaatagatc
      961 gactccccga agtcgaacct cagtgccttc ttgacgaata taaaatactg gaccggcagt
     1021 ggctcattca aaccgaagcg gtcccggatt gaattgagtt ctgtttcatc cgccccggga
     1081 gacgcgatca tcagcgccgg atcgggtccg atccgggtgg ccaaaaaaat catcaccgat
     1141 acaatccata cgacgatgat tccgaagaag aaacgtcgga tgatgtatct tttcatctcc
     1201 cctgactccg ccaaaccctc ccgggtcttc tcccccccac cctgaccgtt cggctgtctt
     1261 cgaccctgag ctcagaccga acggagctca cggcgaagcc cccccctcgg aggggggagg
     1321 gaagggtggg ggtgatatct ctaatattcc ttttcaatgg aggttcttgc aaaattcttg
     1381 gaaacaggaa aaatcgtcat cccgggcaac cccggcgaag gccgggggcg acccggaatc
     1441 caggattctc ggaggattct agattccggc tttcgccgga atgatggagt aaatactttt
     1501 gcaataacct caatatctag gttaatttac gatctcgatg tcttcaaaag gagccgtgaa
     1561 ccagatgagc ggctgaatct tgtacggatt cccctttacc cggggtccga tggccaccgg
     1621 cgagttggtt gaggtaaggg ggatccacat ggttctttca tagatcattt tctggatgcg
     1681 ggccatcaaa tccctgcgcc cctgcgagga ggtttccttc ccgtactgat cccacagggc
     1741 ctggatgtct ggatagttgc cgtaggaacc cccgctgaac atggtggata gcctgcctcc
     1801 aattgtcgga gaggttgggt tgtcaatgaa tatgccccct ttcatcttcc ccccctgccg
     1861 catggccagc agagccggct tatccaggag gacggtctct acgctgatcc ccaccgcttt
     1921 ccagtaagtg gcaatctgct ctccataggg ccaatacccg ccctcgtagg ggtagaattt
     1981 gccggcctga aagccctggg gatagccggc ttcggccagc agcttcttgg ccttctccgg
     2041 atcatagggg tatggcggaa aatcgacggc catcgggtct ccttccaggg cgatattgct
     2101 caccccaccg caaccgggca tatgcacgtc tgccagagtc tggcggtcga tagccaggct
     2161 ggcggccatc cggacccgcg ggtccgccca gggagacttg gggtcccatt gggccgtgag
     2221 gtaggcgacc cacctcaccg ggctcaaggg agccagcatc cggagcccct gggacttttt
     2281 caggtcttcg tagtagaccc cctgcatgag ggttccgatg tcggcttcgc cccgcttgac
     2341 catggccaaa cgggttgccg gctcgggaat gttatagaac tccagtcttt tcacgtgggg
     2401 aactttccgc cagtattcct caaaggcctc tcccaccagc ttgaccccgg gtacatgttc
     2461 cacaaacttg tacggtcccg cgccgatggg atgcttttta aaccccgcat cccccacttt
     2521 ttctaagtat ttcttgggag cgatccatcc aaaagtagtg gacccgggaa ggagatattc
     2581 cagaaagtcg gggaaagatt ccttgaaacg aactcgtacg gagtaaggat tgaccgcttc
     2641 cgctttctcc gtctttccgt ggatggcttt ggccagggag cccttgtatc tcatgaagct
     2701 gaagaccaca tcttcggccg tcagggggtc tccgttgtga aatctcaccc cttgcctcag
     2761 tttaaattcg tagactctgg catcggggct gacggtccac gattcggcga ggctcggggt
     2821 gtagagtcct tgcgccatgg gtttgaacaa ggcgtcatgg aaaagataca tcgggtggta
     2881 agcggggata ggaaaagttc cgaacgctgg gtccagccag tcggccgaaa aactccagtg
     2941 gatggcttgt ttcaacaccc ccgccggggg agcagcccaa acctgcgtgg cactggaaca
     3001 aagaaggaac agcatgaaaa aggtgatctt ctccaatctt ttcttttttc cagtttccat
     3061 ccatcctctc cttctctgaa attggggtga agaagaaagt ttcccaaccg tccaggggac
     3121 tccgggccaa ttcttatcgc gtgcagcaag gcccgtcaca gctgcttttt ccgagcattt
     3181 tctggcagta gggggtcttg ttcaagacct cattcccttt ttcggtgatg aggatgttat
     3241 tttcaatccg aacccccccg cctcccggca caccgggcac cgtaatcgta ggctccaggg
     3301 aaaagatcat ccccggctgg agttcaaaca ccttctcccc ggtggcggcc agctcgccga
     3361 tgatgggata ggtgagcccc gaggtcccga tactgtgccc cagcagccca tggaatccaa
     3421 atttttcaaa accatgcttc tggataatcc cccgggccga ccggttgaca aactcgttgg
     3481 tcaccccggg tttcatggtt tccacgatgt tcatcatggt ctcatagacg gcggtataga
     3541 ttttcttctg ctgctcgctg ggccggccca ggcagaccgt ccgggtgaaa tcggagaaat
     3601 acccgttaaa acaccccccc aggtcgagaa aaaccaggtc tccccggagg atgagtttgt
     3661 ccgaggcgaa acggtggagg ggggcggtat tctcccccga agccacgatc aggctgcact
     3721 ggggcacttc catcccgaag gaataaaggg tttccatggc cgtggccaac acttcgcact
     3781 cccgcacccc gctttcgatt cgacggaaag ccctttccat ggccacatcg gccacctgga
     3841 cgg
//
LOCUS       DASPRR010000275         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_869116, whole genome shotgun sequence.
ACCESSION   DASPRR010000275 DASPRR010000000
VERSION     DASPRR010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869116"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..>2977
                     /locus_tag="VEL68_10755"
     CDS             284..>2977
                     /locus_tag="VEL68_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HXZ36488.1"
                     /translation="MSVHRGPKDTDADWRKREIPEGVLDSDALRINLEETAVEQVAVD
                     PKYNVLQEVVAGYRGIQKTVDALLFELNHPFKNWEVILPEMRAFALKHFASYARHPQG
                     AQAIAVIFDVFLDAAVNSGREALQVKAIDYLLGYLEKVVSEMGEAHRENFLPVLRACF
                     RRLFDLPEKLFFFLATSHIPLKRIAEVLLRKLPEGSELGEFNELLVRSLRAAYGYWLK
                     QEDPRLWFAGDDGGSSRPWKPGTDVFDGISHQRLGHYMGQLEQLSSQDGSVETLRKLL
                     TLPGYMEIVRGYKEIPNRLGPPATEPQAAREDSLSKNWKILSLFKIMEIQGLQDIHEE
                     TLREINHTLVELIRQEPPERMENFLVKTFSLLKSSVENYPQTALQCIQTIGAEVFNRG
                     YSPLVEVFLEQVLHFGFQYPDVQGVDVNWQLICNFCHLVNVRVWLDLITRNPKWCSTL
                     LSALIINLKLGGTCIRDTDLFQKEVTKLLNADVEPVYNLIKQLAKLLPVYFNEIGAEG
                     PLRDVTTEIDEITNRKDPLIHFLRKQSHVESSNLIEDFIKGIFRFWRTKEKSGLRSFL
                     PPEVYGQLEKEGPYIDDAHTLVRNIIRENGLSREEDLLGLTEAQVEAFMAGQPSISEA
                     EKKRVALLIKIYRLVNQKYNLGFQEIRYHLQEASRWGFEGLDQLQKILDRDDTLECLE
                     GILTYLEGLKETIVSPERFEAREDIYRKRHIAVDIPSMYGRYREKKFDALGLTFRLEN
                     LANIYFERLIDFFDLSFITRATLFRVISVIRYFFRAIQLDGISSHRLDMHLKLLEKSL
                     EIKRFSFTQYLDIFRGFSEGVKDIISVYYVNAHKNNLNELIRQMGRKNLLTKYLPSDP
                     VAPSELIHRIGERFLRDLISGTFGLQYLDHFLTRIQLTLSEQ"
BASE COUNT          684 a          834 c          807 g          652 t
ORIGIN      
        1 cgaatggggc tactccaacc gggtgttaga cctcatcttg cacctggatt cggaaaaacc
       61 ggtttagccg ctcagcggta aatgaaacca atcccgccaa gggcgggacg aaataccaag
      121 caccaaatac ccaataggct ccaatgaccc aattccaatt ccgggcaaag atcgttatgg
      181 gcattgggcc ttgggattta tttaaggttt gggatttgtc atttggaatt tgtaactcca
      241 acccttgttg gatcccggct cggttgggtt acgaaagggt ctgatgtccg ttcatcgcgg
      301 tcccaaagat accgacgcgg attggcggaa gagggagatc ccggaggggg tcctcgactc
      361 cgacgccctg cgtatcaacc tggaagagac ggccgtagag caggtcgccg tagaccccaa
      421 gtacaacgtc ctgcaagagg tggtggccgg gtatcgggga atccagaaga cagtcgatgc
      481 cctccttttc gagctgaatc acccctttaa gaattgggaa gtcatcctgc cggaaatgcg
      541 ggccttcgcc ttgaagcatt ttgcgtcgta tgcgcgccat ccccaagggg cgcaggccat
      601 cgccgtaatc ttcgacgttt ttttagacgc agcggtgaat tccggacggg aggctctgca
      661 ggtcaaggcg atcgactacc tcctggggta tctggaaaaa gtcgtgagtg aaatgggtga
      721 ggcccatcgg gagaacttcc tgcctgtttt gcgcgcctgt ttccgccgcc tgttcgatct
      781 tccggaaaaa ctgttcttct tcttagccac cagtcacatc cctttgaagc gaattgccga
      841 ggtcttgctc cgcaaactgc cggaaggctc tgaattgggc gagttcaatg aactcctggt
      901 ccgaagcctg cgggccgctt acggatactg gctcaaacag gaagacccgc gattgtggtt
      961 tgccggagac gatgggggct cctctaggcc ctggaagccc ggaacggatg tttttgatgg
     1021 gatctcgcac cagcgtcttg gccattacat gggtcaactg gagcagcttt cctcccagga
     1081 tggttcggtg gagacgctgc ggaaactgct caccctgccg ggctacatgg agattgtccg
     1141 gggctacaaa gaaattccta accgtttggg ccccccggcc acggaacccc aagctgcccg
     1201 ggaagattcg ctgagcaaga actggaaaat cctctccctt ttcaagatca tggagatcca
     1261 gggtttacag gatatccatg aagagacgct gcgggagatc aaccacacgc tggtcgagtt
     1321 gatccgccaa gagccccccg agcggatgga aaattttctc gtgaagacct tttccctgct
     1381 caaatccagc gtggaaaact atccccaaac cgctctccag tgcatccaga ccatcggggc
     1441 cgaagttttt aaccgcggct acagccccct ggtcgaagtg ttcctggagc aagttctgca
     1501 ctttggattc caatacccgg acgtgcaagg ggtggacgta aactggcaac tgatctgcaa
     1561 tttctgccac ctggtcaatg tccgggtttg gctggacctc atcacccgca accccaaatg
     1621 gtgcagcacc cttctttccg cgctcatcat caacctgaaa ttaggcggca cctgcatccg
     1681 ggacaccgac ctcttccaga aggaagtgac caaactgctc aacgcggacg tcgagcccgt
     1741 ttacaacttg atcaagcaat tggccaaact ccttccggtc tatttcaatg aaattggggc
     1801 cgagggcccg ctgcgggatg tcacgacgga gattgatgaa atcaccaacc gcaaggaccc
     1861 cctgatccac ttcctgcgca agcaaagcca tgtggaaagc agcaacctga tcgaagattt
     1921 catcaagggg attttccgct tctggcggac gaaggaaaag agcggcctcc ggagctttct
     1981 ccctccggag gtctacggac aactggagaa ggaaggcccg tacatcgatg atgcccacac
     2041 cttggtccgg aacattattc gggagaatgg attatccagg gaagaagacc tgctggggct
     2101 gaccgaggcg caggtggaag catttatggc cgggcaacca tccatctccg aggcggagaa
     2161 gaagcgggtg gcgctgctga tcaaaatcta ccgcctggtc aaccagaaat acaacctggg
     2221 tttccaggag attcgatatc acctccagga agcttcccgc tggggcttcg aagggctcga
     2281 ccagctccag aagatcctgg accgcgacga taccctggaa tgcctggagg gcatcctgac
     2341 ctacctggag gggttgaaag agaccatcgt ttcgcccgag cggtttgagg cccgggaaga
     2401 tatttaccgc aagcggcaca tcgccgtgga catcccgtcc atgtacggac ggtaccggga
     2461 gaagaagttc gatgccctgg gcctgacctt tcggctggaa aacctggcca atatctattt
     2521 tgaaaggctg atcgacttct tcgacctttc cttcatcacc cgggccaccc tgtttcgggt
     2581 catctcggtc atccggtatt tcttccgggc catccaattg gacggaatct cctcccatcg
     2641 gttggacatg catctgaagc ttttggagaa gtcattggag attaagcgct tcagcttcac
     2701 gcagtacctg gacattttcc gggggttctc cgaaggggtt aaagacatta tttctgttta
     2761 ctacgtaaac gcccacaaaa acaacctcaa cgaattgatc cgccagatgg gccggaagaa
     2821 tcttctcacc aaatatcttc cgagcgatcc ggtcgccccg agcgagttga ttcaccgcat
     2881 cggggagaga tttctccggg acctcatctc cggaaccttt ggtctgcagt atctggacca
     2941 tttcctcacc cgcattcaat tgaccctctc cgagcag
//
LOCUS       DASPRR010000276         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1510917, whole genome shotgun sequence.
ACCESSION   DASPRR010000276 DASPRR010000000
VERSION     DASPRR010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510917"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..739
                     /locus_tag="VEL68_10760"
     CDS             152..739
                     /locus_tag="VEL68_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXZ36489.1"
                     /translation="MEQKDRDYIVRLFPVVSKIKDEGLRDKVILTWLHAWKLSNFSRI
                     EDVHQFEPARNYIAYTNVDHTNQVCRASEAIAAMASEGLNLKLHMDYLLAGALLHDVD
                     KMVIFDFRTGGFTEMGRRSFHAVAGAAMARSLGLPEEVVHIIEAHSTRFSPHPPKSAE
                     ALILRHADIVVAGLVYKAKGLEMEKVLNESLARIS"
     gene            833..2812
                     /locus_tag="VEL68_10765"
     CDS             833..2812
                     /locus_tag="VEL68_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ36490.1"
                     /translation="MSPFPLLFQTGQIGPMKLRNRIVMAPLGTNLADANGAVTPQMIN
                     WYVERARGGVGLIIVENSLADVRFGRGLARQLLIDDPKLTPGLNELVEAVHGAGSKIA
                     IQINIQGAGVDGELQPGVQPVGPSPISYVFDRSGLGSVLPPRMRREKRLRGLEVEEMK
                     ELRDSFIRAAGIAKSAGFDALEIHGAHGYLLAGFLSAFSNKRDDDYGGDLESRLKYVI
                     EVCQGIREQVGPGFPILFRMSGREYLPGGREIEESQAIARRLEEVGIDGLDISAGISM
                     EALPYTWMNPPTALPQGPFIADAQAIKKAVHIPVIGVGKIRDPWFAEKALQEGRADFI
                     ALGRTLIADPEWSRKAAEGKEDEVRRCISCNRCLRILYRLPIRCAVNARAGLEREFPM
                     VSSPRPRKVAVVGGGPAGMEAACTAAERGHEVTLFEKDRALGGQLRLAIVPPFKKDLV
                     GLLAFLQGQVKKRVNLHLHKEIDAQELLNHLFDVVILATGCTPPVLKNNEDPRVTRAW
                     DVLAGRARLAGSRVVVSGKGRGACETSEFLATRQKKKVTLIHSGPLEELGSELEPIFE
                     RRLLLERLQECGVKILCQTAITRIDPDGVQVEGVTSGRIPCDHIVLDEPPVPDRSLLD
                     QLRGKVKVLGIGDCIDPSDIYRAIHDGFRAGYGIE"
     gene            3053..3442
                     /locus_tag="VEL68_10770"
     CDS             3053..3442
                     /locus_tag="VEL68_10770"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXZ36491.1"
                     /translation="MANREKEMKHHFGVAKFEERANPRFLLNLPVEYYPAKAKTRGKG
                     HTENASEGGLIVYLRKHFKVGDLLKLRVFFSSGPGINTVETVSQVVWTEKLKDAEYRC
                     GMKFVDISPEDMDKLSSFLKKHSPLSH"
     gene            3646..>4276
                     /locus_tag="VEL68_10775"
     CDS             3646..>4276
                     /locus_tag="VEL68_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ36492.1"
                     /translation="MVKKKVLFFYPRFVEEYKALLEKNIPEAEFMICTNREEMEKYAP
                     EAEIALVGMTFPQEIFKRMPRLEWVQALAAGVESYIQNAEQFKRIPVCRITGAFGKYM
                     AEYVLAYVLYFCQNISRVLKAQREKKWDPFRMEFIHQKTLGVMGLGHIGKEVAQKARA
                     AGMQVVSWDMARADVPYVERQFGSREMRDFLKETDFVVLTLPATPATKNL"
BASE COUNT          978 a         1093 c         1236 g          969 t
ORIGIN      
        1 agtatcacaa gatatctctg cgttcggtac atttttttta tttacctcaa gggagttatc
       61 cacagcccct tcgctcacct gctcccgggg gcgcgtcgct ccgggactat ggataactcc
      121 cttgggtaat atacaagggg ggaaggaata aatggaacaa aaagaccggg attatatcgt
      181 ccgtcttttt cccgtagttt ccaagatcaa agatgaaggg ttacgcgata aagtcattct
      241 gacctggctc catgcctgga agctgagcaa tttttctcgg attgaggatg tgcaccagtt
      301 cgagccggcc cggaactata ttgcttacac caatgtcgat cataccaacc aagtgtgccg
      361 ggcctccgag gccatagccg ccatggcttc tgaaggcttg aatctcaagt tgcatatgga
      421 ttacctcctg gctggggctc tcctgcacga cgtggataag atggtaatct ttgacttccg
      481 gaccggcgga ttcacggaaa tgggccgcag aagcttccat gctgtggccg gagcggcgat
      541 ggcccggagc ctggggctgc cggaggaagt ggtccatatc atcgaagccc attccacgag
      601 attctccccc catcccccca agtcggccga agccctcatt ctccgccatg ccgacatcgt
      661 cgtcgccggc ttagtataca aggccaaagg cctggaaatg gaaaaggtgt tgaacgaatc
      721 gctcgctcgg atcagctgag ttttccgaga atggagctgc cctttcctgt gtcgaatcct
      781 cttccacccc gggttttcct tttgaccttt tccaggctgt aggagggatt tcgtgagtcc
      841 attccctctt ctctttcaaa cgggccaaat cggcccgatg aaactcagga accggatcgt
      901 catggccccc ctggggacca acctggcgga tgccaacggg gccgtgaccc cgcagatgat
      961 caattggtac gtggagcgag ccaggggagg ggtcggcctg atcatagtcg aaaattccct
     1021 ggctgatgtt cgtttcgggc ggggactggc ccgtcaattg ctcatcgatg accccaagct
     1081 gaccccgggg ctgaatgagt tggttgaggc cgttcatggc gcgggttcca aaattgccat
     1141 tcaaatcaat atccaaggag ccggggtgga cggggaactt cagccgggag tacagccggt
     1201 gggtccttcc cccatctcct atgtttttga ccggtccggc ctgggaagcg tccttccccc
     1261 gaggatgagg cgggagaaac ggttgcgtgg gctggaagtg gaagagatga aggaactgcg
     1321 cgattccttc atccgcgccg cggggattgc aaaatccgct gggttcgacg cccttgaaat
     1381 tcacggagcc cacgggtatc tcctggccgg gttcctgtcc gccttcagca ataaacggga
     1441 tgacgattat ggaggcgatt tggagagccg gctcaaatac gtcattgaag tttgccaggg
     1501 gattcgggag caggtgggtc ctggctttcc gatccttttc cggatgagcg gtcgggaata
     1561 cctgcctggc ggacgggaga tcgaagaatc ccaggcgatt gcccggcggc tggaagaggt
     1621 cggcatcgac ggcttggata tatcggcggg aatctccatg gaggcgttgc cctatacttg
     1681 gatgaatccc cctacggccc ttccccaggg gccattcatc gcagatgctc aggcaattaa
     1741 gaaggcagtt cacatcccgg tcatcggggt tggtaagatc agagacccct ggtttgcgga
     1801 gaaggcattg caggaggggc gggccgattt tatcgccctc gggcgaaccc tcatagccga
     1861 cccggagtgg tcgcggaagg ccgcggaagg aaaggaggac gaggttcgca gatgcatctc
     1921 ctgcaaccgt tgcctgcgga ttctgtaccg tctgcccatt cgatgcgcgg taaatgcccg
     1981 cgccggcctg gagcgggaat ttcccatggt ctcctccccc cgcccgcgca aggtagcggt
     2041 tgtgggaggg ggacccgctg gaatggaagc cgcttgcacg gccgcggaac gcggtcatga
     2101 agtgaccctc tttgagaaag accgagccct ggggggccag ctcaggctgg ccatcgttcc
     2161 acccttcaag aaagacctgg tgggccttct ggcattcttg caaggccagg ttaagaaacg
     2221 ggtaaatctt catctccata aggagatcga cgcccaggag ttgctgaatc acctgttcga
     2281 cgtggtcatc ttggccacgg gatgtacgcc gcctgtgctg aagaacaatg aggaccctcg
     2341 ggtcacccgt gcctgggacg ttctcgccgg aagggctaga cttgccggga gccgagtggt
     2401 ggtgagcgga aaaggccggg gggcctgcga gacgtctgaa ttcttggcca cgcgtcagaa
     2461 aaagaaagtc accctgattc actccggtcc gctggaggaa ttgggcagcg agttggagcc
     2521 catattcgag cgaaggctgc tgctggaaag actccaagaa tgcggagtca aaattctttg
     2581 tcaaaccgca attactcgca ttgatccgga cggggtgcag gttgaggggg tgacaagcgg
     2641 gaggattccc tgcgaccata tcgtgttgga tgaacccccc gtgcccgacc gttcactcct
     2701 agaccagctt cgaggaaagg tgaaggtcct cggaatcggc gactgcatag acccgtccga
     2761 tatctaccgg gccattcacg atgggtttcg ggcgggttat gggatcgaat aatttcctta
     2821 atccaccaaa tcaaagggct caaattagtt tccccaccta aagagtcttc cccctttgtc
     2881 gccccttcgg ctaccgaaaa accctcctcc attccagcct cgcttcctcg cccagccagc
     2941 cagacatttt tttcttgaca taccatatat tgtatataaa aataatagtt atacaaggta
     3001 tggtattatt ccatggtaat tttttctcat ttttagaatt aaggatggga aaatggcaaa
     3061 ccgggaaaaa gagatgaaac atcatttcgg ggtggcaaaa tttgaagaaa gggcgaatcc
     3121 aaggttctta ttgaatttgc cggttgaata ttacccggct aaggcgaaaa cccggggaaa
     3181 gggccatacc gagaatgcca gtgaaggagg gttgatcgtc tatcttcgca agcattttaa
     3241 ggtcggggat ttgctgaaac tgagggtgtt tttttcttca gggcccggca taaataccgt
     3301 ggaaacggtt tcccaggttg tttggacgga gaaattaaaa gacgcggaat accgctgcgg
     3361 aatgaaattt gtggatatct cccccgagga catggataaa ctgagcagct tcttaaaaaa
     3421 acattctccc ttgtcccact aagattttca atcctcccct cccagtctga tctcgaaaaa
     3481 cctccggcag agccgcagcc ctttcaggtc acttgcccca ttttcaaaca actcaaattg
     3541 aaactgaaaa cctatttgaa gggtgtgctg ccattgtgat aggaatgaat ggatggattt
     3601 caaaaaggat gctatccttg ataggaaaat attgaggaga ttacgatggt caagaagaaa
     3661 gttttgtttt tctaccccag gttcgtcgag gagtacaaag ccctgctgga aaagaatatt
     3721 cccgaggcgg agtttatgat ctgcacgaac cgggaggaga tggaaaagta tgcgccggag
     3781 gcggagatcg cccttgtcgg gatgaccttc ccacaggaga ttttcaagag gatgcccagg
     3841 ctcgaatggg ttcaggctct ggccgcagga gttgagagtt acattcaaaa cgcggaacag
     3901 ttcaagcgca ttcccgtgtg ccggatcacc ggggcattcg gtaaatacat ggccgagtat
     3961 gtcttggcct atgtcctcta cttttgccag aatatctccc gggtgctcaa ggctcagcgg
     4021 gaaaagaaat gggacccttt tcggatggag ttcattcatc aaaagaccct tggcgtcatg
     4081 ggtctggggc acatcggcaa ggaggttgcc cagaaggcca gggctgcagg gatgcaggtg
     4141 gtcagctggg acatggcccg ggccgatgtt ccttatgtgg aacgccagtt tgggtcccgg
     4201 gagatgcgcg attttctgaa agagacggat tttgttgttc ttacgctgcc ggccaccccg
     4261 gcgacgaaga acctgg
//
LOCUS       DASPRR010000277         6446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1675394, whole genome shotgun sequence.
ACCESSION   DASPRR010000277 DASPRR010000000
VERSION     DASPRR010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675394"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VEL68_10780"
     CDS             complement(<1..281)
                     /locus_tag="VEL68_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36493.1"
                     /translation="MKKGLRFGFVLFVFFLIHSASFGFAQQPLMQMVDKGEINWAEQS
                     IRATGSGAPNPDAPNIAAARLGAERAAKADALRNILETIKGVRIDSETLV"
     gene            complement(648..1577)
                     /gene="miaA"
                     /locus_tag="VEL68_10785"
     CDS             complement(648..1577)
                     /gene="miaA"
                     /locus_tag="VEL68_10785"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXZ36494.1"
                     /translation="MRKPKLLAVVGPTATGKSGLTLGLAEKLGGEIISADSVQVYRGF
                     DIGTAKLSPEERQKVPHHLIDILAPEEDYSAASFRNQADQIIQRLQEKGKAIFVVGGT
                     GLYLKVLSRGLFHGPGGDPGLRDSLRQKAEGESEGYLHRELEKVDPETASRLHPHDTS
                     RILRALEVYRHSRKPLSQYQKEHGFRESPYQILKIGLTADRQELYRRIESRVDSMMEK
                     GWLREVQDLLIQGISPRVKPMQSLGYKRIVSFLAGELDWALAVNLIKQDTRRYAKRQI
                     TWFKADPDICWFSAGAENFPAIQEKVLDFFHHC"
     gene            complement(1693..3417)
                     /gene="mutL"
                     /locus_tag="VEL68_10790"
     CDS             complement(1693..3417)
                     /gene="mutL"
                     /locus_tag="VEL68_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXZ36495.1"
                     /translation="MSKIKTLSPDVVAKIAAGEVVERPASVVKELLENALDAGSASAK
                     VEVQGAGRKLIRVTDDGEGMTGEEAIQALQRHTTSKIESLEDLFSLRTFGFRGEALAS
                     IAAVSRLKITTRKEGKLSGTEVRVEGGTLSDTTEMGCPLGTSVEVRDLFFNVPARLKF
                     LKSQGTELGHISEILAKTALANPQARFQLFHEGKLLANYPLRQDPSARLVEALGEEVD
                     GKIFPFRLQQGELRAEGFAGEPGLNRPNSRGIYLFVNRRPVRDRLLTHATLEGYRNVI
                     PKDRYPVAVLLVEVPPPDVDVNVHPGKWEVKFANSETVHRLVIQAVRGMLEETPWLKI
                     KGIPQFEEIRERPTAYLPQGKPISLPLSWPASLRVESKGESQETGRGPVSGITFLGQV
                     QETYLVFASPEGLLLLDQHAAHERVLLEKLSNDLFHGGIPKQPLLLPEVIELPSNESR
                     ILEEHLGDLARMGFDLERGGIRSFWVKSVPQILADRDPLETLRETIKEIYSWGKEGQL
                     QNSYDSLLKMMACHAAIQAHRPMEKEEARALWEDLQNCRFPSHCPHGRPTQLRLSPFE
                     LEKMFGRR"
     gene            complement(3550..4968)
                     /locus_tag="VEL68_10795"
     CDS             complement(3550..4968)
                     /locus_tag="VEL68_10795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXZ36496.1"
                     /translation="MVKHRNRIRNLALFVGDLSSTILAFFLAYFFRDAFPDKSMPYLF
                     PLSWHLNILWPIIPIWSLVFYLMDLYRYWRGPGFWKEVWMVLKAVFLSSLLAGFIIFA
                     LKYQFVSRIFFLSFVFLDLFLVILFRFLVRSGVHLLNRRSEGSRFILIVGLGDDALEM
                     ARGIEKHQELGLRMRGFVSPGETGFPQKWNGYPVLGSAQDLPDILEREIIDEVIFSVS
                     QDELKQMGKLMLFCEERGITTRVILNFFPHLISKTCLEELDGFPLLTFSATPKSELLM
                     FLRRVLDFSGSLVLILLFSPFFLLCTVLIRLDSPGPTWFRQVRCGLNGRKFTFYKFRS
                     MHEGAEERKGELTGFNLMNGPVFKMKNDPRITRVGRFLRKSSLDELPQLFNVLRGDMS
                     FVGPRPLPVAEAEEIKGWQRRRLSMKPGITGLWQVSGRNQIDFHDWVKMDLEYIDRWS
                     FWLDFKILLKTIPTVLSGKGAM"
     gene            complement(4981..6093)
                     /locus_tag="VEL68_10800"
     CDS             complement(4981..6093)
                     /locus_tag="VEL68_10800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ36497.1"
                     /translation="MRIAIVHDHMNQMGGAERVIAAIHETFPEAPIFTTIVDWEILRP
                     ELSNADIRPSWMQKLPGWKKHFKKYLPLFPLAIESMRVKDYDLILSSSSHFAKSAIKG
                     PKALHICYCYTPMRFAWDYENYVKRENLNVLYRSCLPLIIAHLRQWDQQTKDRPDHYI
                     AVSSAVKARIKRVYGRDAEIIFPPVDVQKYRPIERPDNFYLIVSRLNSIKRIELAVEA
                     FNILGLPLKIIGAGPFYKSLKAMASANVTFLGRLPDPEVAEYYAACKAYVFPGEEDFG
                     ITPLEANAAGRPVIAFKGGGALDTVREGLNGLFFDESKSQSLVDAVRSFENGKYNFNP
                     HEIREHALLFDREVFKEKMRLYISRKWTDSLRAGAG"
BASE COUNT         1565 a         1725 c         1648 g         1508 t
ORIGIN      
        1 accagggtct ccgaatcgat gcgaacccct ttgatcgtct ccaggatatt cctcaaggca
       61 tccgctttgg ctgctcgctc cgctcccaac cgggctgcgg caatgttggg cgcgtcagga
      121 ttgggagccc cgctgcccgt agctctgata ctctgctcgg cccagttgat ttctccctta
      181 tccaccatct gcatcaaagg ctgctgggca aaaccaaatg aggctgagtg aattaagaaa
      241 aaaacaaaca aaacaaagcc aaaccgcagt cctttcttca tctttacctc cttcatgtcc
      301 gatcagataa gttttttata aatctctttc gtctctggac cgcccacgaa taaacccgac
      361 atggaaaatg aaatttcagt agaaaaaatc atagatttaa tagccttgtc aaggcaaaaa
      421 gaaatgatcc gttcatcggg cgggttaaat caaattttga aaattttatc gatgcaaaaa
      481 atgggccttc cttagttctt agaaaaagca aaaaaaacaa gggagaagca cccaaacgat
      541 tctcctaact tacgaaaagg aatcagctct ttatcccttt tcggggtaaa tagaactggc
      601 aaaggtcctc catcggtctt cgatcctctg acagggatgg caatttttca acagtgatga
      661 aaaaaatcca aaaccttctc ctgtatggcg gggaaattct ctgccccggc ggaaaaccag
      721 caaatatccg gatcggcctt aaaccaggtg atctgtcttt tggcgtaacg ccgggtgtct
      781 tgtttgatga ggttgactgc caaggcccaa tccaattctc ccgccaggaa agaaacaatg
      841 cgtttatacc ccaaagactg catgggcttc accctgggag aaatcccctg aattaaaagg
      901 tcctggactt cccttaacca ccccttttcc atcatcgagt ctacccggga ctcgattcgc
      961 cgataaagct cttgccggtc tgcggtcagg ccgattttca gaatttggta cgggctctcc
     1021 cgaaatccgt gttccttctg gtattgagaa agaggttttc gggaatggcg atatacttcc
     1081 agggcccgta ggattcggga agtgtcatgg ggatgcaggc gtgaggccgt ttctgggtcc
     1141 accttctcaa gttcccggtg caggtatccc tctgactccc cttcggcttt ctgccgtagg
     1201 ctgtcccgca gaccagggtc tccaccgggc ccgtggaaaa gcccccggga aagaaccttt
     1261 aggtaaagcc cggtaccccc gacgacaaaa atggctttcc ccttttcctg gaggcgttga
     1321 atgatttggt cggcctgatt ccggaaggag gcggcactgt agtcctcttc cggcgccaaa
     1381 atatcgatca ggtgatgggg aaccttttgc ctttcttcag gtgaaagctt ggccgtcccg
     1441 atgtcgaatc cccggtaaac ctggacggaa tcggcgctga tgatctcccc cccgagcttt
     1501 tcagccaggc ccagggtcag tccactcttt cctgtggctg tcggcccaac cacagccagc
     1561 aacttgggtt ttctcatgtc gacccaatcc tcggcgaatg gaacccctca agggggcaaa
     1621 gataatagtt caagagctct ttccgggtct attaagatcg gatgttctga acgcctcagc
     1681 ggagaagaaa aatcatcttc gcccgaacat cttttcgagt tcgaaggggc ttagccgaag
     1741 ctgtgtaggc cttccatggg ggcaatggga tggaaagcgg caattctgga ggtcctccca
     1801 gagggcccgg gcttcttcct tttccatggg acgatgggct tgaatcgcgg catggcaggc
     1861 catcattttg agaagagaat cgtaggaatt ctgcagctgg ccttcctttc cccacgaata
     1921 gatttctttg atcgtctccc gcagggtttc caaggggtct cgatcggcca gaatttgggg
     1981 aaccgatttc acccagaagg acctgatgcc tccgcgttcc agatcaaacc ccatccgggc
     2041 caggtcgccg aggtgctctt ccagaattct tgattcattc gatggtaatt cgatcacctc
     2101 aggaagaaga aggggctgtt tggggattcc cccatggaaa agatcgttgc tcaatttttc
     2161 cagaagaacc cgttcatggg ctgcatgctg atcgagaagt agcaaacctt ccggagaggc
     2221 gaagaccagg taggtttcct ggacctgtcc caagaaggta atccccgaga caggacctcg
     2281 gcctgtctcc tgggattccc ctttcgattc gacccggagg gaagcaggcc aagatagcgg
     2341 gagagaaatc ggctttcctt gcgggagata agcggtcggt cgctctctga tttcttcgaa
     2401 ttggggaatc cccttgattt tcagccaggg ggtctcttca agcatcccgc ggaccgcctg
     2461 gatcaccaaa cgatggactg tttccgaatt ggcaaactta acctcccatt tgcccggatg
     2521 gacgttgaca tctacatccg gtggggggac ctccaccaaa agaacggcta ctgggtaccg
     2581 atccttggga atgacgtttc ggtacccttc cagggtggca tgggtgagca aacggtcccg
     2641 caccggccgt cgattgacaa aaaggtaaat tcccctgctg ttgggccggt tcaagcctgg
     2701 ctctccggca aatccctcag ccctcagttc tccctgctga agccggaaag gaaatatttt
     2761 tccatccact tcctccccca aggcctccac cagcctggcg gaagggtcct ggcggagtgg
     2821 ataattggcc agaagctttc cctcgtggaa gagctgaaat cgggcctggg gattggccag
     2881 agcagtcttg gccaggattt cgctgatatg cccgagctct gtcccctggg attttaagaa
     2941 tttcagccgt gcggggacgt tgaagaaaag gtcccgaacc tccacggagg tgcccaaggg
     3001 gcaacccatc tcggtggtat cagaaagcgt tcccccttcc acccgcactt cagtcccgga
     3061 aagctttccc tctttgcggg tggtgatctt tagtcgggaa acagccgcaa tggaggcaag
     3121 agcttccccc ctgaatccga acgtacggag agaaaaaagg tcttcgaggg actcgatttt
     3181 actggtggtg tggcgttgga gggcctgaat ggcctcttcc ccggtcatgc cttccccgtc
     3241 atccgtgacc cggatgagct ttcgccccgc cccctggact tcaaccttcg ccgaagcgct
     3301 ccccgcatcc agggcgttct ccagaagctc tttcaccacc gaagccggtc tctccacaac
     3361 ttcgccggcg gcgatcttag ccaccacatc gggggaaagg gtcttaattt tgctcatgga
     3421 aagaatctgt gtccctgcca gtgggaatat gaagagattc aatgatccat agtcattttc
     3481 aaggttcaat gttcattgtc cattgatcat tgaccattgc taattgttca ttcctcaagc
     3541 ctgcctctcc tacatcgcac cttttcccga gagcacggtg ggtatggttt tcagtaggat
     3601 cttaaaatcc agccaaaagg accacctgtc gatgtattcc aggtccattt tcacccagtc
     3661 atgaaagtcg atctggttcc gcccgcttac ctgccatagg ccggtgatcc cgggcttcat
     3721 gctcagccgc cttctctgcc accccttgat ttcttcggct tcagccaccg ggagcggccg
     3781 ggggccgaca aaactcatat ccccccgtaa aacgttgaaa agctggggaa gctcatccag
     3841 actgcttttt cttaggaagc gtcctacccg agtgatacga ggatcgttct tcattttgaa
     3901 aaccggcccg ttcatcaagt tgaagccggt cagttctcct tttctctctt ccgctccttc
     3961 atgcatggaa cgaaatttat agaaggtgaa cttccgcccg ttcaagccgc atcggacttg
     4021 tcggaaccag gtgggacccg gactgtccag ccgaatcaga actgtgcaca aaagaaagaa
     4081 tggggaaaac aggaggatca aaacgagact ccccgaaaaa tccaagactc ggcgaaggaa
     4141 catcaacaac tcgcttttgg gcgtggccga aaaagtgaga agagggaagc catccagttc
     4201 ctcgaggcag gttttggaaa taaggtgagg gaagaaattc agaatgactc gggtggtaat
     4261 tccccgctcc tcacagaaaa gcatgagttt tcccatctgc ttcagttcgt cttgagagac
     4321 ggaaaaaatg acctcatcga tgatttctcg ctccaggata tcgggcaggt cttgagcgga
     4381 ccccagaacc ggatatccgt tccatttttg aggaaaacca gtctccccgg gggaaacaaa
     4441 gcccctcatt ctcaatccca gctcttgatg tttttcgatt ccccgagcca tttccagggc
     4501 gtcatcgccc agccccacaa tgagaatgaa acgggaccct tcgctccggc ggttcaatag
     4561 atggaccccg cttctcacca agaagcggaa aagaatgacc agaaacaggt cgaggaagac
     4621 aaaagacagg aagaaaatcc ggctgacgaa ctgatatttc aaggcgaaaa taatgaagcc
     4681 ggccaataag ctggaaagga agaccgcttt cagcaccatc cagacttcct tccagaaccc
     4741 cggccccctc cagtatcggt acaggtccat caggtagaag accagggacc atatgggaat
     4801 gatgggccag aggatattca ggtgccagga aagaggaaag agatagggca ttgacttgtc
     4861 gggaaaagca tcccgaaaga aataggccag gaaaaaagcc aaaatagtgc tggaaaggtc
     4921 tccgacaaac agggccaggt tacgaattcg attccggtgc tttaccatcg gtaaaccctc
     4981 tcatccggcc ccggcccgca acgaatcggt ccattttcga gaaatataaa gcctcatctt
     5041 ttccttgaaa acctctcggt caaaaagcag agcgtgttcc cttatttcat ggggattgaa
     5101 attgtatttc ccgttttcga aagacctgac cgcatccacc agagattggc tcttactttc
     5161 atcgaagaag aggccgttga gcccctctct caccgtatcc aaagcccctc ctcctttaaa
     5221 ggcgatgacc ggccggcctg cggcattggc ctcgagggga gtgatcccaa aatcctcctc
     5281 tccgggaaag acatacgctt tgcaggcggc ataatattcg gcgacttctg gatcgggcag
     5341 ccttcccaag aaagtcacat tcgcgctggc catggccttt aagcttttgt aaaacggacc
     5401 ggccccgatg atctttaagg ggagtcctaa tatgttgaag gcttcgacgg ccagttcgat
     5461 tcttttgatg gagttcagtc tcgatacaat caggtaaaaa ttgtccggtc tttctatcgg
     5521 tctgtatttc tgaacgtcca ccggcggaaa tatgatctcg gcatctcttc cgtaaaccct
     5581 cttgatccgg gccttcaccg ccgaagatac agcgatgtaa tggtcggggc ggtctttggt
     5641 ctgctgatcc cattgcctca gatgggcgat gatcaaagga aggcagctcc ggtaaagaac
     5701 gttcagattt tcccttttca catagttctc ataatcccag gcaaaacgca tgggtgtgta
     5761 gcagtagcaa atatggaggg ccttgggccc cttgatggcg ctcttggcga agtgactgct
     5821 cgagctgaga atcaggtcat agtctttcac ccgcatggac tcaatcgcca gggggaacag
     5881 ggggagatat ttcttaaaat gctttttcca accgggaagt ttttgcatcc aggaggggcg
     5941 gatgtccgcg ttggacagtt ccggtcggag gatctcccag tcgacgatcg tggtgaaaat
     6001 gggagcctcg gggaaggttt cgtggatcgc ggcgatgacc cgctccgctc cccccatctg
     6061 attcatatgg tcatgaacaa tcgctattcg cattgttttt cagtggcact ttattcccag
     6121 tataacggag aaaagcatct ttgaacagac gaaaactttt gagttgagtc ggattgcccc
     6181 tcggaggtaa ggataatggg cttctgccgc cgaaaaatta cctgagggtt tgggtcaggc
     6241 atgaaaagga attgagcggg ccgtgcgcgg aagagattag ggacagaaga aacgcggccg
     6301 aggaaacgaa agagaaaaaa ataggggaat aagtcggggg gccaggtttg gccccccggg
     6361 caggagttac taagcgaaat acctcctcgt catcgacagc ctcttagatt ttttcccctc
     6421 tcaccagctt ccggcaaagg gatcat
//
LOCUS       DASPRR010000278         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1030601, whole genome shotgun sequence.
ACCESSION   DASPRR010000278 DASPRR010000000
VERSION     DASPRR010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030601"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VEL68_10805"
     CDS             complement(<1..947)
                     /locus_tag="VEL68_10805"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXZ36498.1"
                     /translation="MSPWLSIILFLPLLGSFLALVFARRPNVCRWATLLVALGDLALV
                     VGLVAGNLPGRVGPGAKWLLYEDLAWIEHFGIRYSLGLDGISLVLVLLTAFLTALCVL
                     VSWKQIATRVGAFHFFLMLMETGILGVFLANDLFLFYLFWEVQLIPMFFLIGIWGHEN
                     RVYATVKFILYTIAGSLLMLAALIGLFILHGSQTGLYTFSLEQLIQTRLPLATEKWLF
                     AAFLLAFAIKVPLVPVHSWLPDAHTEAPTAGSVILAGLLLKTGAYALLRFGFPLFPTA
                     ARMSVPLLLGLSLVGVFYAAWIALAQKDLKRLVAYSSIS"
     gene            complement(1067..2980)
                     /gene="nuoL"
                     /locus_tag="VEL68_10810"
     CDS             complement(1067..2980)
                     /gene="nuoL"
                     /locus_tag="VEL68_10810"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXZ36499.1"
                     /translation="MQAYLFLTLFPPFLGALTLALAGRLLPRRGTEVLACSTILLSFL
                     TALLALISSGPQAQTVVLLPWIYAEGFSAPMSVLYDRLAGIMAVMVTFVSALIHLYSV
                     SFMRDEEGYVRYFCYLNLFVFAMLVVTFADNLLFLYLGWEGVGFCSYALIGFWYSDEV
                     KATAGRKAFILTRIGDVAFGISIALFFGLYRDLSLATLNQKAPGLDPYLATILGLLLL
                     WAAVGKSAQLPLAVWLPDAMAGPTPVSALIHAATMVTAGVYLLMRFFPVISLSPAAML
                     AIAIVGAFTSLFAACAALAQRDIKRVLAYSTISQVSYMFLGVGAGAVGGSMFHLLSHA
                     FFKALLFLAAGCIIQALHEEHDIFRMGGLRRSLPAVFLLFLAGALSLAAIPPFGGFLS
                     KDQILLAAFNHPEGTYRVLWVLGEVTAFLTALYTFRLFFLVFGEKPASDIAGSSAPPE
                     RGLRPLPKFMVWVLWPLAFLSLAAGALNIPEMLSGREWLAQLLAGVPGAASPFKSSPG
                     VEAAIMVVSGFLSVMGAVWAYFLYHRRPLTEAATPLPQRMKEILFSAFYLDQWYQKVI
                     VMPYQAVSRFLWVNVDQGAVDGGIDRAGKWFPSASLGLLLWTTGRLSTYLKMLFLGFT
                     LLLGAIILGWYPW"
     gene            complement(2981..3286)
                     /gene="nuoK"
                     /locus_tag="VEL68_10815"
     CDS             complement(2981..3286)
                     /gene="nuoK"
                     /locus_tag="VEL68_10815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXZ36500.1"
                     /translation="MIVPFSHVLFLAAILFLLGAVCAVARRNLIMILIGVEIMLNAAG
                     LVFVGASLRWEHLEGQVFVIFILTLAAAEVAVGLALIVTSYRRTGSVNADRYNLLKG"
     gene            complement(3283..>3723)
                     /locus_tag="VEL68_10820"
     CDS             complement(3283..>3723)
                     /locus_tag="VEL68_10820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXZ36501.1"
                     /translation="AVVYLIISFIATGLIFYLLGAPFLALLEIIVYAGAIMVLFLFIV
                     MMLRLTPSQRTWKAALSQWAPAIVLGGFSLVLAAVMIPTDPASNPALKTLTAGPRELG
                     RFLFQKYWFPVEIASFLLLIALVGAFFLGRQEKKAKKTTRQEGK"
BASE COUNT          978 a         1013 c         1187 g          545 t
ORIGIN      
        1 tgggaaatac tggaatacgc caccaggcgc ttgaggtcct tctgggccag ggcgatccag
       61 gcagcataga agacaccgac gagggacagc cccaaaagca gcggaacgga catccttgcg
      121 gcagtgggaa agagggggaa gccgaaccga aggagggcgt aggccccggt cttgagaagc
      181 agtcccgcca aaattacgct gcctgccgtg ggggcctccg tatgggcatc gggcagccag
      241 gaatggacgg gaaccagggg aaccttgatg gcaaaggcaa ggagaaaggc ggcgaacagc
      301 cacttttccg tggccagggg aaggcgagtc tggatcaatt gctcgaggga aaaggtgtac
      361 agtcccgttt gggacccgtg gaggatgaac aggccgatga gcgcggccag catcaggagg
      421 cttccggcga tggtgtagag gatgaatttg acggtggcgt agaccctgtt ctcgtggccc
      481 catattccga tgagaaagaa catgggaatg agctggactt cccagaagag gtagaagaga
      541 aagaggtcgt tggccaggaa gacgccaagg attccggtct ccatgagcat caggaaaaaa
      601 tgaaaggcgc ccacgcgggt ggcgatctgc ttccaggata ccaggacgca gagcgccgtg
      661 aggaaggcgg tcaacaggac cagtaccagg ctgattccgt cgaggccgag gctgtaacgg
      721 atgccgaagt gctcaatcca agccaggtct tcgtagagga gccatttcgc ccccggcccg
      781 acccggcccg ggaggttgcc ggcaactaga ccgacaacca gcgccaggtc gcccagcgcg
      841 accaggaggg tcgcccatcg gcagacgttg gggcggcggg cgaagaccag ggccagaaag
      901 ctgcccagaa gcgggagaaa caaaatgatt gagagccaag gggacataaa aaatgcctaa
      961 aattaagaac caatgcctta agtgcgctcg agtgccttaa gtgccccaag ttaaagagaa
     1021 cgaccggagc ccctgacact cacccttacc cttaccctga cctcacctac catggatacc
     1081 aacccaggat gatggctcct aacaggagcg tgaaccccag gaacagcatc ttcagatacg
     1141 tggaaagccg gcccgtggtc caaagcagca ggcccaggga cgccgaggga aaccatttcc
     1201 ccgcccggtc gatccccccg tccactgcgc cctggtcgac attgacccac aggaaccggc
     1261 tcaccgcctg gtaaggcatt acgatgactt tctggtacca ttggtccagg tagaatgcgg
     1321 agaacaggat ttccttcatc ctctgaggga gaggggtcgc cgcttcggtc agggggcggc
     1381 ggtggtagag gaaataagcc cagacggcgc ccataacgga aagaaacccg ctcacgacca
     1441 tgatggcagc ctccactcca ggggaggact taaatggcga agctgctccg ggcacgcccg
     1501 ccaataattg ggcaagccat tccctcccgg aaagcatctc ggggatattg agagctcccg
     1561 cggccaggga gagaaaggcc aagggccaga gaacccagac catgaatttt ggcagtggac
     1621 gaagacccct ctccggaggg gcggaacttc cggcgatatc cgatgcaggc ttttccccga
     1681 agacgaggaa aaaaagtcgg aaggtgtaga gggcggttaa aaaggcggta acttccccca
     1741 atacccatag cacccgatag gttccttccg ggtgattgaa tgcggccagg aggatctggt
     1801 ccttgctcag gaacccgccg aaggggggaa tcgcggccag ggagagcgcc cctgcaagga
     1861 aaagaagaaa gaccgccgga agggaccggc ggagaccgcc catgcggaag atatcgtgct
     1921 cttcgtggag agcctggatg atgcatccag ccgccaagaa caggagcgct ttgaagaagg
     1981 cgtgagagag gaggtggaac atggacccgc ccacagctcc cgctcccact cccaggaaca
     2041 tgtaactcac ctggctgatg gtggaatagg ccaggactcg cttgatgtcc ctctgggcca
     2101 gggctgcaca tgccgcaaaa agggaagtaa acgctcccac gatggcaatg gccaacatgg
     2161 cagcagggga aagggagatg acggggaaga accgcatgag cagatacacc ccggccgtga
     2221 ccatggtggc ggcatggatc agggcggaga ctggcgtggg tcccgccatg gcatcgggga
     2281 gccacacggc caaaggaagt tgggcggatt ttcccaccgc tgcccagaga aggagcaagc
     2341 cgagaatcgt ggccagataa gggtctaacc cgggagcctt ctggttcagg gtggcaaggg
     2401 aaaggtcccg gtacagccca aagaagaggg cgatggagat tccgaaagcc acgtccccga
     2461 tccgggtcag aataaaggcc ttccgtccgg cagttgcctt gacttcatcg gagtaccaga
     2521 agccgatcag ggcataagag cagaaaccga ccccctccca gcccaggtag aggaagagaa
     2581 ggttgtcggc gaaggtcacc accagcatgg caaaaacgaa gagattcagg tagcagaaat
     2641 aacgaacgta gccttcctcg tcgcgcataa agctcacgga gtaaaggtga atgagggctg
     2701 agacgaaggt caccatcacg gccatgatcc cggccagccg gtcatagaga acgctcatgg
     2761 gcgccgaaaa tccttccgcg taaatccagg gaagaagaac cacggtttgg gcttgcggtc
     2821 ccgaggagat cagggccaag agagcggtca ggaaggagag gagaatagtc gagcaggcca
     2881 ggacttctgt tccccttcga ggcagaagcc tcccagccag ggctagggtc agggctccca
     2941 ggaaaggagg aaataaagtt aagaaaagat aggcctgcat ctatcccttc aggaggttgt
     3001 accggtccgc gttcaccgac ccggtacgac gataagaggt aacaatcagc gccagaccca
     3061 cagccacctc ggcggcggca agggtcagga tgaagatgac gaaaacctgg ccctccaagt
     3121 gttcccaacg gagggaggcc cccacgaaaa ccaggccggc ggcgttcagc atgatctcca
     3181 ccccgatgag gatcatgatc aggttgcgcc gggcgacagc gcaaaccgcc cccagaagga
     3241 agagaatcgc tgcgagaaaa agaacatggc tgaaggggac aatcattttc cctcctggcg
     3301 ggtggttttt ttggccttct tctcctgccg gccaaggaag aaagctccca ccaaggcaat
     3361 gagcaggagg aaagaagcga tctccaccgg gaaccagtac ttctggaaaa ggaacctgcc
     3421 gagctctcgg ggtccggccg tgagggtctt caaggccggg ttgctagccg ggtcggtcgg
     3481 gatcatgacc gcggccagaa ccaacgagaa tccgcccaga acgatggccg gagcccactg
     3541 ggacagggcg gccttccagg tcctctggga aggggttaac ctcagcatca tcacgataaa
     3601 gaggaatagg accatgatgg ccccggcata gacgatgatc tccagaaggg cgaggaacgg
     3661 ggcccccagc aaataaaaga tcaggccggt ggcgataaag gaaatgatga ggtaaaccac
     3721 cgc
//
LOCUS       DASPRR010000279         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_379723, whole genome shotgun sequence.
ACCESSION   DASPRR010000279 DASPRR010000000
VERSION     DASPRR010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379723"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1363
                     /locus_tag="VEL68_10825"
     CDS             386..1363
                     /locus_tag="VEL68_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36502.1"
                     /translation="MSRRILFNALAVIAFLWGASYVGAQSVNYPTKPVILQVPWPAGG
                     STDVGARIVAAIAEKKMGQPIVVTNRVGAGSQIGLTETARAKPDGYTLGFASLPALNT
                     IVLDPERKALFDLNSLVFIINQVIDPGIIWAKGDSPYKTLKDLLEDARKRPGEIRAAT
                     TGILGDDHLAILMLEQAAKVKFRIVHFDGGAQVFTATLGGQVDVAFGNVGDIGTKLKG
                     TPLRILMLMDSQRSKFVPDIPTTAELGFPGIISSSSRGMFGPAGIPDPILKKIQAVFL
                     EAMKDPDHLDKMDKAALAVKPLVGEEYKKYVYELHQRITPLVEIARKEK"
     gene            complement(1582..2934)
                     /locus_tag="VEL68_10830"
     CDS             complement(1582..2934)
                     /locus_tag="VEL68_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ36503.1"
                     /translation="MQPNPSPNVTDRLAGFFVETSYEALPPEVIHEGKRCIVDGIGVA
                     LGGADHPSSHILLKYCRTLGSQPEAQAWGHPDRLPVELAALVNSHQGHVLDFDDTFLP
                     PETALHGTVPLLSPLLAVAEHRHLSGKAVLRAFVLGFEAEARLALALGRNHLLGGWHV
                     TATAGPVGGAVGVGVLIGLSPARMRNAIGIAITHSGGVTAMFGSMSKAYQCGKAAEAG
                     ARAALLAEMGFDSGMEPLTHPQGYLNVASSDRAFEKLTDQLGERYLIRENGFKPYASG
                     VVTHPLIDALVQLRKEGMDADSVARIEVRVNPFVLQVTGQSEPKTGLEGKFSAYHCAA
                     VALLDGTARKAQFTDARVNDPAAVTLRRKVALIPDESLRKDECTVVVKLLHGPKQERH
                     VPRGSGTVENPMSDEALAEKFLDLAQTLIGLRAAQVLDLVWTLDSLPDAAHLPLLLKG
                     "
BASE COUNT          736 a          837 c          918 g          677 t
ORIGIN      
        1 caactgcgtc tgcccccttg ccgattggga gagcacaatg aatatgtgta caagcaagtg
       61 atcggcgttt ccgacgagga atatactgaa ctggagaagg ctggtcatat tgggatggat
      121 ttcattccca gcataccgta aagcgcgtca gcattcaaat tcatagaata taaatactca
      181 tgccggatac aagaataatg gatatgctgg gaatcaaatc ccccatcggt taaaacgccg
      241 tcccgtcgaa gctcaagccg cttcgttgaa gcgagccgat ggtatttgtc ttgatcctaa
      301 atcccttttt gcgtaaaatc agatcaagcc gcaaacaggt attcttaagc agagggctaa
      361 aatttattcc gcggagggct aaaaaatgag cagaaggatt cttttcaacg ccttggcagt
      421 aattgctttc ctttggggag ccagttatgt cggagctcag agcgtaaatt atcccaccaa
      481 gccggttatt cttcaggttc cttggccggc gggcggctcc acggatgtgg gtgcccggat
      541 cgttgcagcg attgcggaaa agaaaatggg gcagccgatc gtcgtcacca atcgagtggg
      601 tgccgggtcg cagattggct tgaccgaaac cgcccgagca aaaccagatg gctatacttt
      661 ggggttcgcc agccttccgg ctctgaacac catcgttcta gacccggaac ggaaggcgct
      721 atttgacctg aattccctgg ttttcatcat taaccaggtc atcgatccag ggatcatctg
      781 ggccaagggc gacagtccct ataagacctt aaaagacttg cttgaagacg cccggaagcg
      841 ccccggtgaa atccgggccg ccactaccgg aatcctcggg gatgaccacc tggccatcct
      901 tatgctggaa caagcggcta aggtaaaatt ccggattgtt catttcgacg ggggcgccca
      961 ggtcttcacc gcgacattgg gggggcaagt ggatgtggcc ttcggcaatg tcggagatat
     1021 tggcaccaag ctcaagggga cgccgctccg gattctgatg ctgatggact cccaaaggtc
     1081 gaagtttgtg cccgatattc ccacaactgc cgagttgggc ttccccggaa ttatttcttc
     1141 ttcttcccgc ggcatgttcg gtcctgccgg tattccggac ccaattctga aaaaaatcca
     1201 ggccgtcttt ttggaggcca tgaaagaccc ggatcatttg gataagatgg ataaggccgc
     1261 gctggcggtc aaacccctgg tgggcgagga atataagaaa tacgtttacg agctacatca
     1321 gcggattact cccctggtgg aaatcgctcg aaaggaaaag tgattgggga agaagaaaaa
     1381 gtgatttgcg tcgtaaattt tcgtactcaa cgataaaaga ggtggtaccc agaaatcaga
     1441 aggcccctat tcagtgaaaa gcggttcgtg cagaaaatgc ttaacaaatt caattcaccg
     1501 tcctgccttg ggaaaaggct aaacctgttc tgcccgaacg tacccaaact cgtggggtct
     1561 cctggaaatt tttctcgcgg gttatccttt caataaaagg ggaagatggg cagcatccgg
     1621 aagactgtcc aaggtccaca ctaagtccag gacttgagcg gcgcggagac cgatgagcgt
     1681 ttgggccagg tcaagaaact tctcggccag ggcctcatcg gacataggat tctctacggt
     1741 gcccgagccc cgggggacat ggcgctcctg cttggggccg tggagaagtt ttaccacgac
     1801 ggtgcattca tctttcctca aagactcgtc gggaatcaag gcgactttgc ggcggagtgt
     1861 cacggcggcc gggtcgttca cccgcgcgtc ggtgaactgg gccttgcggg cagtgccgtc
     1921 gaggagggcc acggcggcgc agtgataggc gctgaatttc ccctccaagc cggtcttcgg
     1981 ttccgactgg ccggtgactt gcaagacgaa gggattcacc cggacttcga tgcgcgcgac
     2041 ggaatcggca tccatgcctt ctttccggag ctgaaccagg gcatcaatca agggatgagt
     2101 gaccactccg ctggcgtaag gtttgaagcc attctcgcgg atcaggtatc tttcccccag
     2161 ttgatcggtc agtttttcga aggcgcgatc gctggaggcg acatttaagt atccctgagg
     2221 gtgagtgaga ggttccatcc cgctgtcaaa tcccatttcc gccagaaggg ctgcccgagc
     2281 tcccgcctcc gccgccttcc cgcactgata agccttagac atagagccga acatggccgt
     2341 caccccgccg ctatgagtga tggcgatgcc aatggcgttc cgcatccggg cagggctcaa
     2401 gcctatcagg acccccactc ccacggcccc cccgaccggg cccgcagtcg cagtcacatg
     2461 ccatccgccg aggaggtggt ttcgcccgag ggcgagggct aggcgggcct cggcctcgaa
     2521 gcccagaaca aaggcccgca ggactgcttt gcccgacaga tggcggtgtt cggcaacggc
     2581 cagcaacggg gagaggaggg ggacggttcc gtgcagcgcc gtttccggcg gcaggaaggt
     2641 gtcgtcgaaa tcgaggacat gcccctgatg actgttgacc aatgctgcga gctcgaccgg
     2701 cagccggtcc gggtgtcccc aggcttgggc ttcgggctga ctccccagag tgcgacagta
     2761 tttcagaagg atatgggaag acgggtgatc ggctcctcca agggcgactc cgatcccatc
     2821 gacgatacag cgtttgcctt cgtggataac ctccggagga agagcctcat aagaggtttc
     2881 gacgaagaat ccggcgagcc ggtcggtcac gttgggcgaa ggattgggtt gcatgaaggt
     2941 ctctctaatg acgaatcggc caagagacgc tggacgattt ccttgatgtc tctctgactt
     3001 ccccccgttc tcgttgagac ccgcgtatct ttaaggatca aatgcttgaa taaatccaaa
     3061 aatacagaat ggactgatta tgggggagga aaaaaaggtt gtcaagaaaa tccggggaag
     3121 ggaaaaggct ccgcggtgca taggatataa agccgcgtcc tctgtggc
//
LOCUS       DASPRR010000280         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_712276, whole genome shotgun sequence.
ACCESSION   DASPRR010000280 DASPRR010000000
VERSION     DASPRR010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712276"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VEL68_10835"
     CDS             <1..183
                     /locus_tag="VEL68_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36504.1"
                     /translation="LYTFDLSLMDDLEGIHSKVGELSPQNPPEKWNRAIEEANHLLDA
                     LEKKFRQRNDFMARPA"
     gene            225..1355
                     /locus_tag="VEL68_10840"
     CDS             225..1355
                     /locus_tag="VEL68_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXZ36505.1"
                     /translation="MSQFLEVIEWFDESGLEMVHRIPESGSAEIKFGAQLIVRESQAA
                     VFFRDGKGYDVLGPGRHTLSTMNLPILTKVLSLPFAFKSPFRVEMIFTNLKVFTHLKW
                     GTQEPVAFKDRELGVVRLRGFGNYTMRIIQPLLFINTLIGTQGVFATDEISGYLRDVI
                     VSRLNDLLGETLQSIFELPRNYDEFGVALKARVSQDFQKYGLELVDLFVNSITPPDDV
                     QKMIDERSGMQAVGNLDSFLKFKAAKAMGDAAKAEGGAAGTAAASGMGLGVGAGMGMM
                     IPGILMKTMEEGSRAVPSTKQLQCPKCHAQIPFEARFCPFCGHQIVKINKCLHCGKDL
                     PLEANFCMVCGNKVEKSDLSCSKCGTKALPGATFCNQCGEKL"
     gene            1512..>2389
                     /locus_tag="VEL68_10845"
     CDS             1512..>2389
                     /locus_tag="VEL68_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36506.1"
                     /translation="MDAAQESQARILIHCPQCGGDITFLEESQVIHCEFCGSSLVVAG
                     REGVLRYVLPAQLRDPQAAAAQGVEFLRKAGRLSPRAVEAFLFYAPFWRIRGMAYRWI
                     FGLKPMKVEIQSGVPPPMERLKVLLTRVLDHTIPGYGGLEIGLSTLGVRTQALRLQPF
                     GREHLQKRDSFLPLEVSLEQAQVEAELLANAFFEGEELIPEVILHRLVGKIFSVIYFP
                     IWGVEYQHNQGREMLLLDAVAGSGLGSLPDGSRIQQKLKGEESRKSFQFSEIRFLPFR
                     CPNCGWEFPFRPLSLL"
BASE COUNT          523 a          652 c          673 g          541 t
ORIGIN      
        1 ctttatacct ttgatctgtc ccttatggat gatctggaag ggattcattc taaagtcggt
       61 gaattgtccc ctcagaatcc tccagagaaa tggaaccggg cgatagaaga agcgaatcat
      121 cttctggatg ccttggaaaa gaaattccgc caacgaaatg acttcatggc ccggcctgcc
      181 tgattcgggg gtagaacccg ggccggccgg aggaggagga acgaatgagt cagtttttag
      241 aggtgatcga gtggttcgac gaaagcggcc tggagatggt gcatcgcatc cctgagtccg
      301 gatccgcaga aatcaagttt ggagcccaac tgatcgtccg ggagagtcag gccgcagtct
      361 ttttccggga tggcaaggga tacgacgtcc tcggccccgg ccggcacact ctttctacca
      421 tgaacctgcc cattttgacc aaggtcctga gcctgccttt tgctttcaaa agcccctttc
      481 gggtggagat gatcttcacc aacctgaagg tatttaccca cctgaaatgg ggaacccagg
      541 aaccggtggc tttcaaggac cgtgagctgg gagtcgtccg cctcagagga ttcggcaatt
      601 acaccatgcg catcatccag cctctgctct tcatcaacac cctgattggc acccagggag
      661 tctttgccac ggatgagatc tccggttacc tgcgcgatgt catcgtttcc cgcctgaacg
      721 atctcttggg ggaaactctt caatccattt tcgagcttcc ccgcaactac gatgaattcg
      781 gcgtagccct gaaagcccgg gtgagccagg acttccaaaa gtatggcctg gagctggtgg
      841 atctgttcgt caatagcatc acccccccgg acgatgtcca gaagatgatc gatgaacgga
      901 gtgggatgca ggctgtagga aatttggact ccttcctgaa attcaaggcc gccaaggcca
      961 tgggggatgc ggccaaagcc gaggggggag cggcgggcac tgcggccgct tcggggatgg
     1021 gccttggggt cggggcggga atggggatga tgatccccgg tatcctgatg aaaaccatgg
     1081 aggaagggag cagagctgtg cccagtacaa aacaattaca gtgtcccaag tgtcatgcgc
     1141 agatcccttt tgaagcccgc ttctgcccct tctgcggtca ccagatcgtt aagatcaaca
     1201 aatgcctcca ttgtgggaaa gatctccccc tggaggccaa cttctgcatg gtctgcggca
     1261 acaaggtcga aaagtcggat ctctcctgct ccaagtgcgg caccaaagcc ttgcccggag
     1321 ctaccttttg caaccagtgc ggggaaaagc tttaactcat agggcgcaga ttttcaccga
     1381 tgtacgcaga tagaaactac gcctataggc attggaacag cacctgttgg caaggccttt
     1441 gcgatcttag gagatcatta atatcggtat ttatctgcgt tcatctgcgt cccaaaaata
     1501 ataattagac catggatgct gcccaagaat cccaagcccg gatcctgatc cattgtcccc
     1561 aatgcggcgg ggacatcact ttcctggaag agtcccaggt catccattgt gaattctgcg
     1621 gctcttcgct cgtcgtggcg ggaagggagg gcgttctgcg gtacgttctc ccggctcagc
     1681 ttagagaccc tcaagcggcc gcggcccagg gggtggagtt tttacggaag gcgggcaggc
     1741 tctcgccgcg cgcggtagag gcgttcctat tctacgctcc tttctggcgt atacggggaa
     1801 tggcgtaccg ttggatcttc ggcctgaaac ccatgaaggt tgaaattcag tccggggtcc
     1861 cccctccgat ggaacggctg aaggttctcc taactcgggt actggaccac accattcccg
     1921 gatatggggg cctggaaata ggtctttcca ccctgggcgt ccgcacccaa gcgcttcgcc
     1981 tccagccctt cgggcgggag catctgcaaa agagggattc ttttcttccc ctggaggttt
     2041 cgctggaaca agcccaggtc gaagcggagc tcttagccaa tgccttcttc gaaggggaag
     2101 aattaatccc cgaagtgatc ctgcaccgcc tggtggggaa aatcttctcc gtgatctatt
     2161 tccccatctg gggcgtagag taccagcata atcagggtcg agaaatgctg ctcctagacg
     2221 ccgtcgcagg aagcggtttg ggctccctcc ccgatggatc gcggattcaa caaaaactca
     2281 agggggagga gagccgcaag tctttccaat tcagcgagat tcggtttctt cctttccgct
     2341 gtccgaactg tggctgggaa ttccctttcc gccctttgag cctgttgca
//
LOCUS       DASPRR010000281         7135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1676506, whole genome shotgun sequence.
ACCESSION   DASPRR010000281 DASPRR010000000
VERSION     DASPRR010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676506"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1349
                     /locus_tag="VEL68_10850"
     CDS             165..1349
                     /locus_tag="VEL68_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36507.1"
                     /translation="MRFWFTEEQEKFRQEVRDFLQAELKAGTFVAKSGGLAEGSSQEF
                     SKKMAQRGWIGMTWPKQYGGQGRSYVDKVILNEEIFKVQAPVAYHFLADRQIGPSIIH
                     FGSDWQKEYFLPRFVRAEENMKFCLLFSEPNAGSDLVNVATSATKDGDSYLIRGQKVW
                     TSGGHQSEYGWLLARTCFDPKVPRHESCSQFILDLRLPGVTVRPIINMAGRHTFNEVF
                     FDDVRVEKRFMVGPENEAFKQIMAQMDYERSGVERILQNYPIYQKLMEYVKKMEKKKV
                     GEAFYSWVRDSVAQLETEFQIGRLLVYYTAWTIDQGRKPSKEAALAKAYCNQYEQRLN
                     DLATRVLGPSSLLREGTPWAAFDGELAECYLWSPSYTLQGGSVEVLKNIVAQRGLNLP
                     RK"
     gene            1401..2534
                     /locus_tag="VEL68_10855"
     CDS             1401..2534
                     /locus_tag="VEL68_10855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36508.1"
                     /translation="MDFNLSDESKMLKSNAEKFMKEKSPSSFVKNILKEEKGFSEALW
                     KEMAGLGWLGLIYAEPYGGSGGNFFDLFVLFEEMGKAVFPSPFFCSAVFAGLLINEAG
                     NESVKKAYLPGIIEGKKILTVGLQNEQFFYDYQKPALEARGDGKDSFRIRGTRLLVPY
                     AHVADETLVCAKVMKGPRSSGSTLFITKAKDAGIQLTPLNTITGEKSFAVTYADYPAS
                     SKDILGSLGKGAAYVEKVMPKLIALKCGEMVGGLSKVVDMTVDYVKQRVQFGKPLGVL
                     QVVQHYCADMTTLLDTARMIAYQAASLISAGLPCAKEVAMAKAWCSDAYKRATQVAHQ
                     LHGGIGFTEEHDLHLYYKHAKALEMEFGDSWVHRQRVAEAMGL"
     gene            2626..3828
                     /locus_tag="VEL68_10860"
     CDS             2626..3828
                     /locus_tag="VEL68_10860"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ36509.1"
                     /translation="MDEQSHFPNPLAGVKVLDLSHVQSGPTCTVILADMGADVIKVES
                     FTGDQFREPMEGANFFNFNRNKRGIALNLKAKEGLEIALKLARKADVLVENFLPGSMD
                     RLGLGYENISRINSGIVYCSISGFGQSGPFWDRPAYEPILQAMSGIMDATGEPGRPPV
                     RIRPAMVDYCTGANAAFAIAAALFAREKTGRGQRIDVALLDVALFAMAPYVTQFLRKG
                     ELPERAGSAQPSGGAIQNFETRDGLIYVVAGTDHMFKNFCKALNREDLAKDPRHATRK
                     LRAENRLEIAEVLTRETRKYSSAELEAKLLAADVACGSVRTIGDIVREPHVRARGILE
                     EAEYPKVGKVVAVKTPLFLSGKAAPHRRRAPLLGEHTGEVLKELGYSDSEIENLIRSG
                     VARQPEAK"
     gene            3841..4635
                     /locus_tag="VEL68_10865"
     CDS             3841..4635
                     /locus_tag="VEL68_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ36510.1"
                     /translation="MEPKELILEKEGHVAVLTLNRPDKMNAISLKMRVDLPLALQEVQ
                     EDDDLRALVITGAGKGFCSGADVAVQAARAAGQQAETSRKTILQLTGALILGFEKINK
                     PVIAALNGIAAGVGLTLALASDIRLASSQARFSAIWVKRGLIADGGGSLLLPLTVGME
                     KALELCFTGEVIDAREAERIGLVSRVVPPEELMSRAKELAQKIVANPPISVELVKRVM
                     WEKVRNQLREALFFESYAQGVCRTTQDQKEAVKAFMEKREPVFKGL"
     gene            4680..5708
                     /gene="dctP"
                     /locus_tag="VEL68_10870"
     CDS             4680..5708
                     /gene="dctP"
                     /locus_tag="VEL68_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ36511.1"
                     /translation="MKRMTALIMGLSWGILMSGLGMAYGQQPIELKWASPYPIGHPSY
                     VMALEFIDYIHKRTANRVKVVHFPAEQLGKAKDLLIVCSQGVADLAQVHITYFAGQLP
                     INNVAQLPFYTKASEGSKIYRRLIDSCPEIQQEFKKYGVRGLEGHTTAQYDVATIKKP
                     VKTPDNLKGLKLKTAGGMYDKIAKRYGINPVSIAASETYEAMQRGIVEGCIFNFPSIR
                     SYRLNEQIKYITNGMRCGGYPGAVIVNEKKWEKLPAEIQKGIIAASQDATNRWGPYWD
                     QMHDEAVKQFQNQGIQVYFIKPEERAKWDAPLKGVEEEYIKEVEKKNLPGRKVFQDFL
                     KIAQEVTK"
     gene            5876..>7135
                     /locus_tag="VEL68_10875"
     CDS             5876..>7135
                     /locus_tag="VEL68_10875"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXZ36512.1"
                     /translation="MSMREMLAELKKERERILNLASPKAIERQHSRGKMTARERINFF
                     YDPETFVELGLFAKPRITGFDIDQQDITGDGVITGSGMVDGRPVYSYAQDFTVAGGSQ
                     GSVHGKKALRVMKQALKARVPCVHMIDSGGVRVQDAVTRDLADSYQLFFYYHTISSGV
                     IPQIGLLMGPCAAGAAYSPILTDFLIMVKGTSHMYIASPTLIKSVQFVDVTEEEIGGP
                     KMHAEVSGCCDVLAENDRDCLQKCKDLLSYLPSHNQEDPPLVPLGDDPNRRDEELLDM
                     VPINSKKWFDMHRVIERIVDKGKYFEIKPDYAKNMITLFARLDGRSVGIVANNPKHKA
                     GAIDINAADKEARFIRFCDCFNIPLIFLVDNPAYLPGADQERGGIIRHGAKVLHAISE
                     ATVPKFTVYIRKAYGGGNPAMCSEPLGS"
BASE COUNT         1735 a         1862 c         2106 g         1432 t
ORIGIN      
        1 atagcgaatc ccaagatgtc gatccgcacc ctgaggagga ttgaactcga gctcaacaaa
       61 tggcagatct ggacggtcca ggggtcttga ggaatctgtt ggggaaatat ttaagaaggt
      121 gatgctgggt gatgatccag cctaagggaa aggagacgga gaacatgaga ttctggttta
      181 ccgaggagca agaaaaattt cgtcaagagg tccgtgattt tttgcaggcc gaactcaagg
      241 cgggaacctt tgtggccaaa agcggagggc tggcggaagg aagcagccaa gagttctcca
      301 aaaagatggc ccaacggggc tggatcggga tgacctggcc caaacaatac ggaggccagg
      361 ggcgctctta cgtggacaag gtgattctga acgaggaaat attcaaggtc caggctcctg
      421 tggcctatca ttttttggcc gaccgccaga tcgggccctc gatcatccat tttgggtcgg
      481 attggcagaa ggagtatttc ttgccgcgct ttgtaagggc cgaggaaaac atgaaattct
      541 gcctcctctt tagcgagccc aacgccggtt cagacttggt gaacgtggcg acttccgcca
      601 ccaaggacgg ggactcttac ctcatccggg ggcagaaggt ctggacttcc ggcggccatc
      661 agtcggaata cggctggctc ctggcccgga cctgttttga ccccaaggtc ccgcgccacg
      721 agtcctgttc ccagtttatc cttgaccttc gcctccccgg agtgaccgtc cggcccatca
      781 tcaacatggc cggtaggcac acctttaacg aggtgttttt cgacgatgtg cgcgtagaaa
      841 aaagattcat ggtgggcccc gaaaatgagg ccttcaagca gattatggcc cagatggatt
      901 atgaacgatc cggcgtcgaa cggatcctcc aaaattaccc catttaccaa aagctcatgg
      961 agtacgtgaa gaagatggaa aagaaaaaag tcggagaagc cttttattcc tgggtccggg
     1021 attccgtggc ccagctggaa acggagttcc agatcggccg gctcctcgtt tactacaccg
     1081 cctggaccat cgaccagggc cgaaagccat ccaaggaagc ggcattggcc aaggcttact
     1141 gcaaccagta tgaacagcgc ctcaatgacc tggccacgcg ggtcctcgga ccttcgagcc
     1201 tcctgcgcga ggggactcct tgggccgcct ttgatggaga gctggccgaa tgttaccttt
     1261 ggtcgcctag ctacaccctc cagggaggga gcgtggaagt attgaagaac atcgtggccc
     1321 agcgcggact caatttgccc aggaagtaaa ccccggaccc tgctggaatg agaccctcta
     1381 gatagaaaag gagcgcaaaa atggatttca acttgagcga cgaaagcaag atgctgaaaa
     1441 gcaatgcgga aaaattcatg aaagagaaaa gtccgagttc attcgtgaaa aacatcctga
     1501 aggaagaaaa aggattttct gaggcccttt ggaaggagat ggcggggttg ggctggctgg
     1561 gactcatcta tgccgaaccc tacgggggaa gcgggggaaa cttttttgat ctttttgttc
     1621 tatttgaaga aatgggcaag gccgtgttcc cgagcccctt tttctgcagt gcggtctttg
     1681 ccggcctgct catcaacgaa gccggcaatg agagcgttaa aaaagcctac ctgccgggga
     1741 tcattgaagg aaaaaagatc ctgacggtgg gccttcaaaa cgaacagttt ttttatgact
     1801 accagaaacc ggccctagaa gcccgggggg atgggaagga ttccttccgg atcaggggaa
     1861 cccgcctgct cgttccttat gcccacgtcg ccgatgaaac tctggtatgc gccaaggtca
     1921 tgaaaggtcc caggtcttcc ggttcaacgc tattcataac gaaggccaag gatgcgggga
     1981 ttcaattgac tcccctcaac accatcaccg gagagaagtc ctttgcggtg acctacgccg
     2041 attatccagc cagttctaaa gatatccttg gatcactcgg taaaggcgcc gcgtatgtag
     2101 aaaaggtcat gcccaaactg atcgccctca aatgcgggga aatggtcgga ggattatcca
     2161 aggtagtcga tatgaccgtc gattacgtca agcagagggt ccagttcggg aagcctctcg
     2221 gagtgttgca ggtcgttcag cattactgcg cggatatgac gaccctctta gatacagcgc
     2281 ggatgattgc ctatcaggcc gcatccctca ttagcgccgg cctcccctgc gcgaaggagg
     2341 ttgccatggc caaggcctgg tgcagcgacg cttacaagcg agccacccag gttgcccatc
     2401 agctccacgg cggaatcggg ttcaccgaag agcatgacct gcacctctac tacaagcatg
     2461 ccaaggccct ggagatggaa ttcggggatt cttgggttca ccggcagaga gtggccgagg
     2521 cgatggggct gtagataaaa ggatagaagg gaaaacgacc gggccgggta gcatccaatc
     2581 aagggtcggc gaagcacaga aaataaggaa gcgggggttt cgtagatgga tgagcaaagt
     2641 catttcccca atccccttgc cggggtaaaa gtcctggatt tgagccatgt ccagtcaggt
     2701 ccgacctgca ccgtcatact ggcagatatg ggcgcggacg ttatcaaggt cgaatctttc
     2761 accggggatc aatttcggga gcccatggag ggggctaact ttttcaattt caaccggaac
     2821 aagaggggaa tcgccctgaa cttgaaagcc aaggagggcc tagagatcgc cttaaaactg
     2881 gccaggaagg ctgacgtcct tgtggagaat ttcttacctg ggtccatgga ccggttggga
     2941 ttgggatacg agaatatttc ccggatcaat tctggaatcg tttactgctc catctccggg
     3001 ttcgggcaga gcggtccttt ctgggaccgg cccgcctatg aacccatcct tcaggccatg
     3061 tcgggaatca tggacgccac gggcgagcct ggacgtcctc cggtcagaat ccgcccggct
     3121 atggtcgatt attgtaccgg ggccaacgcc gctttcgcca ttgccgcagc tttattcgcc
     3181 cgggaaaaga ccggacgcgg gcaacgaatc gatgtggcct tgctggatgt ggccctcttt
     3241 gccatggccc cctatgtaac ccaattttta cggaaggggg agctgcccga gcgggcgggg
     3301 tcggcccagc cctctggggg ggcgatccag aatttcgaaa cgcgggatgg cctgatctac
     3361 gttgtggccg ggacggatca tatgtttaaa aatttctgca aggccctaaa ccgcgaggac
     3421 ctggctaagg acccccgaca tgccacccgg aagctcaggg cggagaaccg gctcgaaatc
     3481 gcggaggtcc tcacccggga aacgcgcaaa tactcgagtg ccgagctgga ggccaagctg
     3541 ctggccgccg acgttgcctg cggaagcgtc cgcaccatcg gagacatcgt tcgggaaccc
     3601 catgtccggg cccggggcat cctggaagaa gcggaatacc ccaaagtagg gaaagtcgtc
     3661 gctgtcaaga cgcccttatt cctatccgga aaagcggcac cccatcgcag gcgcgccccc
     3721 ctgctgggag agcataccgg ggaagtcctg aaggagctcg gctacagcga ttcggaaatt
     3781 gagaatttga tccggagcgg ggtcgcccgg caaccggaag cgaaatgagg aggggagagc
     3841 atggaaccga aagaactcat cctggaaaag gagggccatg ttgcggtcct gactttgaat
     3901 cggccggata aaatgaacgc gatcagcctg aaaatgcggg ttgaccttcc ccttgccctg
     3961 caggaagtcc aggaggatga cgaccttcga gctctggtca tcaccggcgc aggaaaggga
     4021 ttctgttcag gggcggatgt ggccgttcaa gcggcgcgtg ccgcgggaca acaggcggaa
     4081 accagccgaa aaaccatcct ccaactcacg ggggctttga ttctggggtt tgaaaagatt
     4141 aacaaaccgg ttatcgcggc gctgaatggg atcgccgccg gagttgggtt gacccttgcc
     4201 ctggcctccg atattcgcct tgcttcgagt caggcacgtt tctctgccat ctgggtcaag
     4261 agaggcttga tcgccgatgg gggggggtcg ttattgctgc ccttgaccgt gggcatggaa
     4321 aaagccctgg agctatgttt caccggcgag gtcatagatg cacgggaggc cgaaaggatc
     4381 ggcctggtaa gccgagtggt cccccctgaa gaactgatga gccgggcaaa ggagctggcc
     4441 cagaaaattg tggccaatcc gcccatatcc gtagaattag tcaaacgggt catgtgggaa
     4501 aaggtccgca accagttgcg ggaagctttg tttttcgaga gttatgcgca gggagtctgc
     4561 cggaccaccc aggatcaaaa ggaggcggtc aaggctttta tggaaaaacg agaaccggta
     4621 tttaaagggt tgtgatcgag agtttgcccg gattcattat cttaggaaag gagcaacgga
     4681 tgaaaaggat gacggcgcta atcatggggc tgagctgggg gattctcatg agcggcctgg
     4741 ggatggccta cggacagcag cccattgagc tgaaatgggc aagcccctac cccatcggtc
     4801 acccttccta tgtgatggca ctggagttta ttgattacat ccacaaacgc acggccaata
     4861 gggttaaagt ggtccacttt cccgcggaac agctgggaaa ggccaaggac ctgctgatcg
     4921 tctgcagcca gggggttgcg gacctcgctc aggtccacat cacttatttt gccgggcagc
     4981 tgcccataaa taacgtggct cagcttccct tttataccaa ggcctcggag ggatcgaaga
     5041 tttaccgtcg cctaatcgat tcatgcccag agatccagca ggagtttaaa aaatatgggg
     5101 tgaggggact ggaaggccac accacggccc agtacgatgt ggccaccatc aagaagcccg
     5161 tcaaaacccc cgataatctc aaaggcctga agctgaagac cgccggcggg atgtacgaca
     5221 agatcgccaa gcggtacggg atcaacccgg tcagcatcgc cgcttccgaa acctacgagg
     5281 ccatgcagcg gggaattgtg gaaggctgta tcttcaattt tcccagcatc cgttcttacc
     5341 ggttgaacga gcagattaaa tacattacca acgggatgag atgcggcggg tacccgggcg
     5401 cggtcatcgt aaacgagaag aaatgggaga agctgccggc ggagatacaa aaaggtatta
     5461 tcgccgcctc tcaggatgcc accaatcgat ggggccctta ttgggaccag atgcacgatg
     5521 aagcggtcaa acaattccaa aaccagggaa tccaggtcta ctttatcaag ccggaggaaa
     5581 gggccaaatg ggacgccccc ttgaaagggg tcgaggaaga atacatcaaa gaggtggaaa
     5641 agaaaaatct tcccgggaga aaggtctttc aagatttctt gaaaattgcc caggaagtga
     5701 ccaagtaaat ttgtcttccc tttccccggc cggagcgtgt ggatgcactc agcctttcct
     5761 cccagggggt accctggctc cggcacaaag aagggcagcg ggagttgaat gatgcacgaa
     5821 aaaactaaga ggaacacagc cccaggtttc tctatttgaa ggaggtttgt atctcatgtc
     5881 catgcgggaa atgcttgcgg aacttaaaaa agagcgggaa agaatcctca acctggccag
     5941 tcccaaggcg atagaacggc aacacagccg cgggaagatg acggcgcggg agcggatcaa
     6001 ctttttctat gacccggaga cttttgtgga gctgggtctc tttgccaagc cccgaatcac
     6061 cgggttcgac atcgaccagc aggacattac cggcgacggg gtgatcaccg ggtccgggat
     6121 ggtcgatggc cggccggtgt actcctacgc ccaggatttc accgtcgccg gagggagcca
     6181 ggggtcggtc cacggaaaaa aagcgctccg ggtgatgaaa caggccctga aagcacgggt
     6241 tccctgcgtc catatgatcg attcaggagg agtcagggtt caggatgccg tcacccggga
     6301 cctcgccgat tcctaccaat tgttttttta ttatcacacc atctcctccg gggtaatccc
     6361 tcaaatcggc ctgctcatgg ggccctgcgc ggccggggcc gcctattctc cgatcctgac
     6421 ggatttcctc atcatggtaa agggcaccag ccacatgtac attgcctccc ccaccctcat
     6481 caagtcggtc caattcgtgg atgtgaccga agaggaaatc gggggtccca agatgcatgc
     6541 cgaggtaagc ggctgctgcg atgtgctggc ggaaaacgat cgggattgcc tccagaaatg
     6601 caaggacctt ttgagttatc tgccatccca caaccaggag gaccctcccc ttgttcccct
     6661 gggggatgac ccgaatcgcc gggacgaaga gctgctggac atggtcccca tcaattccaa
     6721 gaaatggttc gatatgcatc gggtcatcga gcggatcgtc gataagggga aatatttcga
     6781 gatcaagccc gattatgcca agaatatgat caccctattt gcccggttgg acggacggtc
     6841 ggtcggaatc gtggccaata atcccaagca taaagccggg gctatcgaca tcaatgcagc
     6901 ggacaaggaa gcgcggttta tccgtttctg cgactgcttc aacatcccct tgatctttct
     6961 ggtggacaac cccgcttacc tgccgggggc ggaccaggag aggggcggca tcattcgtca
     7021 cggggcgaag gtgctccatg ccatctcgga ggccacggtt cccaaattca ccgtttatat
     7081 tcgtaaagct tacgggggtg gaaacccggc catgtgcagc gaacccctgg gttct
//
LOCUS       DASPRR010000282         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_712884, whole genome shotgun sequence.
ACCESSION   DASPRR010000282 DASPRR010000000
VERSION     DASPRR010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712884"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VEL68_10880"
     CDS             complement(<1..182)
                     /locus_tag="VEL68_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36513.1"
                     /translation="MSLFLSREAVIFLAVAAAICVTLGSVLRFIIRNPVRRLAAYLIY
                     LGYILFAASIAIYIVLG"
     gene            complement(185..1102)
                     /locus_tag="VEL68_10885"
     CDS             complement(185..1102)
                     /locus_tag="VEL68_10885"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36514.1"
                     /translation="MVRSEETREAKKPGSISLSGRVEVNSISKSYGMGPLRQMVIEDC
                     SFNVESGKLTVLIGPSGCGKTTLINILAGYEAVDSGTLIMNGEPIHGPGWDRLVVFQE
                     TALFPWMTTLENVMYGHLVRRERNKKELEAEAMGLLKKVGLADFRDKYPLQLSGGMQR
                     RAELVRALINTPQVMMMDEPFRGLDAMTRELMQEYYLRLFEETQITNLFVTSELEEAI
                     FLADRLIILSNRPARVRKILDINLPRPRELKVLTSDEYLDYKKQALEILHVEAMRAFA
                     TGSRLAVADFLEAYAEMGKKPEEKGKELH"
     gene            complement(1130..1945)
                     /locus_tag="VEL68_10890"
     CDS             complement(1130..1945)
                     /locus_tag="VEL68_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36515.1"
                     /translation="MSGDKVTQYLGKNLRKTCDALISKTVYRGFTAIVIAVLFWEAAA
                     RLGLPIIGKIPAPSSVISSLNTLIRDTYYWESWLVSFKRVFLGFVVAQIVGIPLGLFM
                     GIRRYFRDLTFPIIEVMRPIPPLAWVPTAVIFWPTPEMSMTFVTFLGAFFTMVLNILG
                     GVRNIDIRYIRAAQSLGSTNRDIFRRIVLPATLPSLFTGMTVGMGITWEVVVAAEMIA
                     SRSGLGFLTWKSYVAGDFPAIVIGMISIGVAGYISSSLIRYMGSRFTPWLKII"
     gene            complement(2184..>2383)
                     /locus_tag="VEL68_10895"
     CDS             complement(2184..>2383)
                     /locus_tag="VEL68_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXZ36516.1"
                     /translation="KARVEHPFGHIKRNLKADAFLMRGREGVSAEASVLATCFNLARM
                     ISLLGVSGLIAKLGICTPALA"
BASE COUNT          585 a          664 c          611 g          523 t
ORIGIN      
        1 cccaaaacaa tgtagatcgc tatgctggct gcaaaaagaa tgtatcctag gtagatcagg
       61 taagcggcaa gtcgccttac cggattccgg atgataaacc ggagaacact tcccaaggtg
      121 acacagattg cagcggcaac agccaggaag atcaccgcct cccgcgacaa aaagagggac
      181 aaggtcaatg aagttctttc cccttttctt cgggcttctt ccccatctcg gcataagcct
      241 ccagaaaatc ggccactgct aaccggctgc cggtggcgaa cgccctcatg gcctctacgt
      301 ggaggatttc cagagcttgc ttcttatagt ccaggtattc gtcagaggtc aacaccttca
      361 gttctcgggg tcgagggagg ttgatatcga gaattttccg aacccgggcg ggtcggttgc
      421 tgagtatgat gagccgatcg gccagaaata tcgcttcctc gagttctgaa gtcacgaaga
      481 ggttggtaat ctgagtttcc tcaaagaggc gaaggtagta ttcctgcatc agctcccggg
      541 tcatggcatc gagaccccgg aagggctcat ccatcatcat cacctggggt gtattgatca
      601 aagcccgaac caattccgcc cggcgttgca tccccccgga aagctgaagg gggtatttat
      661 cccggaagtc ggccaagccc accttcttca gcaaccccat cgcttccgct tccaattctt
      721 ttttatttcg ctccctccgg accagatgcc cgtacatgac attttcgagg gtggtcatcc
      781 agggaaagag agccgtttcc tgaaagacca ccaagcgatc ccatcctgga ccgtggatcg
      841 gttcgccgtt cataatcagg gtccccgaat ccacggcttc atacccagcc aagatgttga
      901 tcaaagttgt cttcccgcag ccggacgggc cgatgagtac ggtcagcttg ccgctctcca
      961 cattgaaaga acaatcctca atgaccatct gacggagggg tcccattcca taggatttgg
     1021 agatggagtt gacttccacc cttccggaaa gagaaatgga acccggtttt ttcgcctctc
     1081 tcgtctcttc tgatcgaacc atatccaccc tatcccgcct taaagggaat caaatgatct
     1141 tcagccaggg cgtgaagcgg ctccccatat accggatcag ggaactggaa atgtaccccg
     1201 caactccaat gctgatcatg ccgatcacga tggccgggaa atctccggcc acataggatt
     1261 tccaggttaa aaagccgaga ccggagcgac tggcaatcat ttccgcggca acgaccactt
     1321 cccaagtgat gcccataccc acggtcattc cggtgaacaa gctagggagg gtggccggga
     1381 ggacgattct ccggaagata tcccggtttg tggaccccag ggattgggcg gcccgaatgt
     1441 agcgaatgtc gatgtttcga acccccccca ggatattcag aaccatcgta aagaaggccc
     1501 cgagaaaggt gacaaaggtc atactcattt ccggggtggg ccagaagatg accgcggtgg
     1561 ggacccaggc caaagggggg atgggccgca tcacctcgat gatgggaaag gtgaggtccc
     1621 ggaagtaccg tcgaattccc ataaaaagac ccaggggaat gccgacgatc tgagcaacca
     1681 caaagcctag gaacacccgc ttgaagctga ccagccatga ttcccaataa taggtgtccc
     1741 ggatcaaagt gttcagggag gagatcaccg aactgggtgc tgggattttg ccgatgatcg
     1801 ggagccccag gcgggcggct gcttcccaga aaaggacggc aatcacaatg gcagtaaatc
     1861 cccggtagac cgttttgcta attagggcat cgcaggtttt tctcaggttc ttccccagat
     1921 actgcgtaac tttgtccccc gacatataat tctcgttcct cccggttcta tactactaac
     1981 gtttagtatc tgaaattttt attaaacaaa aagcatcccc cccaccctaa ccctcccccc
     2041 tcggaggctg tgtcataatt gggtgaaggg ggctgaaaat acagactgtt ctttgaaaac
     2101 tgcatagaat ttgaaggcgt tcagggtaat ttgggggaga aggatttttc ccaatcagta
     2161 tcggacttct cctccctgga atcctaggca agtgcgggtg tgcagattcc gagctttgcg
     2221 attaatcccg aaacgcctaa caggctgatc atccgggcca ggttaaaaca cgtggccaac
     2281 acggaagcct cagcactgac tccctcccgt cctcgcatca aaaaggcgtc cgccttcagg
     2341 ttcctcttga tgtgcccaaa gggatgttcc acccgggctt tcc
//
LOCUS       DASPRR010000283         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2160453, whole genome shotgun sequence.
ACCESSION   DASPRR010000283 DASPRR010000000
VERSION     DASPRR010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160453"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VEL68_10900"
     CDS             complement(<1..508)
                     /locus_tag="VEL68_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36517.1"
                     /translation="MERKIKKVAVLGSGVMGATIAAHLANVGIPSIMLDIVPPKLTPE
                     EEKKGLTFKDAAVRNRFAVTGKQNLLRMRPAALAVPELASLIEVGNFEDHLSRLAEVD
                     WIVEVVVENLKIKQELFKKVAAIRKPGTIVSTNTSGIPIKDICAGMDPEFKQYFLGTH
                     FFNPPRYMK"
     gene            complement(663..1091)
                     /locus_tag="VEL68_10905"
     CDS             complement(663..1091)
                     /locus_tag="VEL68_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXZ36518.1"
                     /translation="MKMEYQVGFLLSRATWAMNNAVNRMVRENGHGDISVAYFAVLQA
                     LWESDGLSITDLGEKAQLEKSTMTSLIDRMESAGLVRREDHPTDRRAYQICLTPRGKE
                     LEKKLDEVVARAYRHLTRGIAEEDLQKAIKVCKRLIQNAD"
     gene            complement(1299..1850)
                     /locus_tag="VEL68_10910"
     CDS             complement(1299..1850)
                     /locus_tag="VEL68_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36519.1"
                     /translation="MSICRFLPLIPFLLSLGCATLPPPPSADSPLIPLQKANLEAVFE
                     EQEGLEVYWENAKEVRDFYRGGVQQKAKAEKDYLDKKYPQALKQYDSSNDFLMVVLKY
                     TSEDDCEYTLFEGTSILFFPNLLIADNDLKAGKILKEMNRDSSARRKWKRALPYVQKS
                     LQFEKTEWGLAVQQELHSLLGTR"
     gene            complement(1850..2152)
                     /locus_tag="VEL68_10915"
                     /pseudo
     CDS             complement(1850..2152)
                     /locus_tag="VEL68_10915"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            complement(2165..2398)
                     /locus_tag="VEL68_10920"
     CDS             complement(2165..2398)
                     /locus_tag="VEL68_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXZ36520.1"
                     /translation="MKVKSWMTWGVTSLAWLILGSQVGDPVWAQGGGGMVFQSFAKAA
                     PLPDFSLEDLQGKTVQIKDHLGKVILLNFWATW"
     gene            2701..2859
                     /locus_tag="VEL68_10925"
     CDS             2701..2859
                     /locus_tag="VEL68_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36521.1"
                     /translation="MKKLISLVLAVAVLASFTLGCAGYVGKETKVKCPKCGAVFTVDE
                     GLKTLGGQ"
     gene            2904..3506
                     /locus_tag="VEL68_10930"
     CDS             2904..3506
                     /locus_tag="VEL68_10930"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ36522.1"
                     /translation="MLIGGERRFFPSFFYFLYILILVSGVCLSPLQTAAQKSEKEALS
                     QFDRLAEEATQLNKRGRFDEVISLLEPHKSDKSNDSALFFNELGIAYRNRGKLTEAVQ
                     AYREAHARDPQNPVILNNLGYALYLKKEYPLAIEQFEKVIQTSPRFKEAHSNLALAYY
                     QLQKYDDALKEIDILLKLDPGHENAKKFRENIRQKMQEKK"
BASE COUNT          881 a         1060 c          949 g          949 t
ORIGIN      
        1 gcttcatgta ccggggcggg ttgaaaaagt gagtccccaa gaagtactgt ttgaattccg
       61 ggtccatgcc ggcgcaaata tcttttatgg gaatccctga ggtgttggta ctcacgatcg
      121 tccccggctt gcgaatggcg gccacttttt tgaagagctc ctgcttgatc ttcaggttct
      181 ccaccaccac ctcgacgatc cagtccactt ccgccaggcg ggagaggtgg tcttcgaagt
      241 tgcccacctc tatgaggctg gccagctcgg gaaccgctaa ggcggcgggc cgcatgcgaa
      301 gcaggttctg tttccctgtc acggcgaagc ggttccgcac cgctgcatcc ttgaaggtga
      361 gccccttctt ttcttcctcc ggggtcaatt tggggggaac gatatccagc atgatcgagg
      421 ggatgcccac gttcgccagg tgggcggcga tggtcgctcc catgacccca gaccccagta
      481 cggcgacttt tttgattttc ctttccatgc ttcctccgat ctgataggtc aactgtccga
      541 gtcttccggg tttttcggac cccggaaacc atctcaaacc ctgttttatt aaaggtcttc
      601 ctgcggcggc cgccggtcca gcctttccct tccgctcccg gatcacctcc ttctctgcct
      661 cctcaatcgg cgttctggat caaacgcttg caaaccttga tggctttctg caggtcttcc
      721 tcggcaattc cccgggttag gtgcctgtag gcccgagcta cgacctcatc caattttttt
      781 tccaattcct tcccccgggg ggtcaggcag atctggtaag cccgccggtc agtcggatgg
      841 tcctcgcggc ggaccagccc tgccgattcc atccggtcaa tcaggctggt catggtggac
      901 ttttccagct gggccttttc ccccaggtcg gtgatactca acccatcgct ctcccacagg
      961 gcctggagca cggcaaaata agcaacggaa atgtctccat ggccgttttc gcggaccatg
     1021 cggttcacgg cattattcat ggcccaggta gccctggaga ggagaaatcc cacctgatat
     1081 tccattttca tgggttgacc cttccccctt aaagatgaaa gagatggata aaatagaaag
     1141 ttcgattgca tactatatga gataatacta atatgtcaag agttatgttg aaggccatga
     1201 aggatcggcc cttcaggctc ttaaggagcg tcatatgcaa ggtattactc taggccagaa
     1261 ccagatgcgg aatgggggag ggaattgggg gatttgggtc agcgagtccc gagcagagaa
     1321 tggagttcct gctgcaccgc aagaccccat tcggtctttt caaattgcag agacttctga
     1381 acataaggca gggctcgttt ccatttcctt cgggccgagc tgtctcgatt catctccttc
     1441 agaatcttac cggctttcag atcgttgtcg gcaatcagca gattggggaa gaaaaggata
     1501 ctggttccct cgaaaagggt gtattcacaa tcgtcttcgc ttgtatattt caggacgacc
     1561 atcagaaaat cattggacga atcatactgc ttcaaggctt gcgggtattt tttatccaag
     1621 taatcctttt ccgctttggc cttctgctgc acccccccgc gataaaaatc ccgcacttct
     1681 ttggcatttt cccagtagac ctccaaacct tcctgctcct caaagaccgc ctccaagttc
     1741 gccttttgca gaggaattaa gggggaatct gccgaaggcg ggggagggag ggtcgcgcat
     1801 cccagagaca acaaaaaggg aatcaggggc aggaacctgc aaatggacat cacttctccg
     1861 ccaataactt gtcgataatg cttaccgctt ccactcccgt ccaatctgcc gaacccatgg
     1921 cccggtagcg aaccattcct ttcacgtcca ctaggaaaga agtcggatga gcccagaccc
     1981 ccaggagttt gcctacctta ttcttttcgt ccaggaggat gggcagggga gaaaggttct
     2041 tttccagaaa tttttggatc gtttcccggc tttctttcgt atccacacgg aaaaagacca
     2101 accctttgga ttggtactgg gcgcacatcc tttccagaga aggagcctcc ttccggcaat
     2161 tgggtcacca tgtggcccag aagttgagga ggatcacttt ccccagatga tccttgatct
     2221 ggaccgtttt cccctgaagg tcctcgaggg aaaaatcagg aagaggcgcg gccttggcaa
     2281 aggactgaaa aaccatcccc cccccgccct gagcccagac cgggtccccc acctggcttc
     2341 ccaggataag ccaagccaga ctcgtaactc cccaagtcat ccaacttttg actttcatgt
     2401 ttccccaacc tcttatggcg atgcaggcac gcttgtttta taacatagga gtcccttccg
     2461 cgcaaggacc tccattcctc cctccggagt gatcctttac ggccattgga tattagaaaa
     2521 gacggttttc tcattgattt ttttgctgaa tttttttctt gactaaaaaa tcattatttg
     2581 ttagctttac actatcttct ttccatcccc ggcccgcggg gctgcggccg gagaaacggc
     2641 ttcaaaattt cccttgggaa ttcgggaata gatctgaaaa atgaaaggag gtgaagggca
     2701 gtgaaaaagc tgatcagctt ggttctggca gtcgcagtac tggcgtcctt tacccttggc
     2761 tgcgccggct atgttggcaa agaaacgaaa gtgaaatgcc cgaagtgcgg ggcagtattc
     2821 acggttgatg aaggtctgaa gaccctcgga ggacagtaga ctccgaatca ggcaggtttt
     2881 tcgttcccaa atttatgaac gggatgctca tagggggaga acgaaggttc ttcccctctt
     2941 ttttttattt tctttatatc ctgattttgg tttcgggggt ctgcctttct cccctgcaaa
     3001 ccgcagccca gaaatccgaa aaagaagccc tctcccaatt cgaccgtctg gcggaagaag
     3061 ccactcaact gaataaacgg ggaaggttcg atgaggtcat atccctgctt gagccgcata
     3121 agtctgacaa gagtaacgac agcgccctgt tcttcaacga gttgggaatc gcttaccgaa
     3181 accgggggaa actcactgaa gccgttcagg cttatcgtga agcccatgcc cgggaccccc
     3241 aaaacccggt catcctgaat aatctcggtt atgcccttta ccttaagaaa gaataccccc
     3301 tggccattga acagtttgaa aaagtcatcc agacctcccc tcgattcaag gaagcccatt
     3361 ccaacctggc cctggcctat taccaactac agaaatacga cgatgcgctc aaggagatcg
     3421 atatcctcct gaaactcgac ccgggccacg aaaacgccaa aaagttccgt gagaatattc
     3481 ggcagaagat gcaggagaag aagtaagatc aataggggtc cgggccatgt tcggattgcc
     3541 gcacaatcgg ttagtgatat ggcccgcata gtcgcttggt tgtaatctcc tccgctttga
     3601 atgttgattg ggccctcccc cgttctttgg aatttggaag gcctatttct gcttcaggca
     3661 cgcgaatcga tctcgatagg gattttatgg caagtggacc aaaccaggca gggcattgac
     3721 cgcgcgcctg attgactagt ggacatcgcg accctactcc ccaatgacta gaaaaatcat
     3781 ccacatcgac atggacgcct tctatgcctc ggtggagcag catgacaacc cggctctcc
//
LOCUS       DASPRR010000284         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1032090, whole genome shotgun sequence.
ACCESSION   DASPRR010000284 DASPRR010000000
VERSION     DASPRR010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032090"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VEL68_10935"
     CDS             <1..418
                     /locus_tag="VEL68_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36523.1"
                     /translation="TVGFGNPKEVKEMSRLAVTEFPDVSFSIHLHNTRGLGLANSLAA
                     YEAGIRVHDGAVGGLGGCPFAPGAKGNTSTEDLVFMFEEMGVKTGVNMDALLEASRYF
                     QKVKKDVRYSSSIIEVGVPVPKGPITKDGSEQKWNV"
     gene            512..1495
                     /locus_tag="VEL68_10940"
     CDS             512..1495
                     /locus_tag="VEL68_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXZ36524.1"
                     /translation="MKKFSLMVGILVIMLALGGGLVLAASYPSKALEFIAPANPGGGW
                     DLTCRSSAKVLQEAKIVPVPITVINKPGGFGAVVWNDIVRNRRTDDHTLVAFSSVLTT
                     QLALKNIKGTYKDITPIASLFVDYGVIAVKKGSPYKTLKDLLAAIKQNPAQVSAAGSS
                     PPGGLDHLKLCLMAQAFGIVPKDIRYVAFDGGADALAALLGGHVTAFLGDAGEIASHS
                     EAGTVLVLATLSPQRLHGIYKNTPTAKEAGVDVISGNWRGFYGPPEMPKPAVAYWEQA
                     LGKMVKEAGWKQILDQNAWIDLYQAGDKLQGWLTKELADYEKILTGLGMVK"
     gene            1492..1950
                     /locus_tag="VEL68_10945"
     CDS             1492..1950
                     /locus_tag="VEL68_10945"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ36525.1"
                     /translation="MNRGNRVWGIIICALGAFYLFEGFNLPPAAIGDPLGPLTFPTIL
                     GVSFIACGVYLAVKPGPRPTQPALVHRYFLQVLMLFALLLLYSASISWLGYLLATFLF
                     VLAGAFLMGERSWGKGVLFSAAFSAGMFILFVRMLTIPLPFGIMKMLGFK"
     gene            1961..3451
                     /locus_tag="VEL68_10950"
     CDS             1961..3451
                     /locus_tag="VEL68_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ36526.1"
                     /translation="MGETLQQLIFGFSIALEPINLLYAFLGCLVGTIIGVLPGIGPAG
                     GMALLIPLIYGKSAASAVILLAGIYYGAMYGGSTTSILLNLPGEAASVVTTLDGYQMA
                     KQGRAGPALCIAAIGSFIAGTLSVVLLMFIGPPVAHFALQFGPAERFALMVFGLSTVT
                     SLAGSSLVKGAVATLLGLMLATMGVDLPTGINRYTFGIDKLMEGFDIIVAAIGLFAVS
                     EIFMSLEEIVAGTLEQIKVTRIWLSMKDFLDSLGAILRGTGLGFVVGAIPGAAHVTAS
                     FFSYTLEKKFSKNPENFGKGEIRGVAGPESANNAAASGALLPLLTLGLPATTVTAVLY
                     GALLMVGVKPSPFLFQKNPDVVWGLVASMYVGNIMLLILNLPLVGVFVRILSIPTRWL
                     LPIVLAISFVGVYSVHNSALDLVLASILGFVGYLMRKFDFPLAPVILGLVLGDLMEQS
                     VRQALMISGGNAAILFQSAIAKTLFALAVFVVVGPMLMQKMRKKEP"
     gene            3598..>3789
                     /locus_tag="VEL68_10955"
     CDS             3598..>3789
                     /locus_tag="VEL68_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HXZ36527.1"
                     /translation="MKEHLAKLLEELSLLDGLPGQEQPVVRYLKEKLQPLAEEVHIGV
                     NGNIYARKTGRKPGLKVLVS"
BASE COUNT          746 a         1049 c         1122 g          872 t
ORIGIN      
        1 cacggttggg ttcgggaacc cgaaggaagt aaaagagatg tcccggctcg ccgtgaccga
       61 attcccggat gtctcctttt ccattcacct gcacaatacc cgggggttgg gcctggccaa
      121 ttcgctggcc gcctacgaag ccgggattcg cgtccatgac ggggcggtag gcgggctggg
      181 cggctgtcct tttgcccccg gggcgaaagg aaacacctcg acggaagacc tggtgttcat
      241 gttcgaagag atgggagtca aaaccggggt gaacatggat gccctcctgg aggcctcgcg
      301 gtatttccag aaggtgaaga aggatgttcg ctattcgagc agcattatcg aagtgggtgt
      361 ccccgttccc aaagggccga tcaccaagga cgggtcggaa cagaagtgga atgtgtaggt
      421 taagaatggg atttcatttt ctggaaagcc tcttaatggg acgccctctc gcggggcgat
      481 ttcccgagta aaccaaaaga ggaggtggaa gatgaagaag ttttcattga tggtaggaat
      541 tctcgtgatc atgctggccc tgggcggagg tttggttctg gccgcttcat acccgagcaa
      601 ggctctcgaa tttatcgcgc cggcaaaccc cggaggggga tgggacctta cctgccgttc
      661 cagcgccaag gtgctgcagg aggcgaaaat cgtaccggtg cccatcaccg tgatcaacaa
      721 accaggggga ttcggagcgg tggtgtggaa tgacattgtg cgcaaccggc ggaccgatga
      781 tcataccctc gtggccttct cgtcggtttt gaccacccag ctggcgctaa agaatatcaa
      841 ggggacttac aaagacatta ctcccatcgc ctccctcttc gtggattacg gggtgatcgc
      901 ggtcaagaag ggctctccct acaaaaccct gaaagacctt ttggcggcca ttaagcaaaa
      961 tcccgcccag gtcagcgccg ccggcagttc gcccccgggg ggtctcgatc atctcaaact
     1021 atgcctgatg gcccaggcct tcgggattgt tcccaaggac atccgctacg tggccttcga
     1081 tgggggagcg gacgcccttg ccgcccttct gggagggcac gtgacagcgt tcctgggcga
     1141 tgccggggag attgcctcgc acagcgaggc cggaactgtc ctggtcctgg ccactctttc
     1201 tccccagcga ctacacggga tctacaagaa cacccccacg gccaaggaag cgggggtgga
     1261 cgtgatctcg ggcaactggc gcggttttta cggcccgccg gagatgccga aacccgccgt
     1321 ggcctactgg gaacaagctt tgggaaagat ggtgaaagaa gcaggctgga agcaaattct
     1381 cgatcagaat gcctggattg acctttatca ggcgggcgac aaactccagg gctggttgac
     1441 caaggaattg gcggactacg aaaagatcct cacggggtta gggatggtta aatgaatcgg
     1501 ggaaaccggg tctgggggat cataatctgc gccctggggg ccttctacct gtttgaagga
     1561 ttcaacctgc cccctgcggc aatcggggac cccctgggcc ctttgacctt cccaacgatc
     1621 ctgggagttt ctttcatagc ctgcggggtc tacttggcgg taaagccagg accccgccca
     1681 acccagcccg ccctggtgca ccgctatttc ctccaggttc tcatgctttt tgcgctcctg
     1741 ctcctttact cggccagcat ttcctggctt ggttacctat tggctacatt tctgttcgtc
     1801 ctggcggggg cctttctcat gggggagagg tcatggggga aaggggttct cttctccgcc
     1861 gccttcagcg cgggcatgtt tattcttttc gttcggatgt taacgatccc gctacctttt
     1921 gggatcatga aaatgctggg tttcaagtaa ggaacgcgcg atgggagaga ctctccaaca
     1981 actcatcttt ggtttttcga tcgccttgga gcccatcaac cttctttatg cctttttggg
     2041 ttgcctggta gggaccataa tcggggttct gccggggata gggccggctg gaggcatggc
     2101 cctgctgatc cccctgatct acgggaaatc cgcggccagc gcggtcattc ttctggcggg
     2161 aatttattac ggggccatgt acgggggctc gactacttcg attctcctca atctgcccgg
     2221 cgaagccgcc tcggtggtta caactctgga cggctatcaa atggccaagc aaggaagggc
     2281 agggcccgcg ctctgcatcg cggcgatcgg gtcgttcatc gcggggacgc tcagcgtagt
     2341 cttgctgatg ttcatcggcc ccccggtggc tcattttgcc ctgcaattcg ggccggcgga
     2401 aaggtttgcc ctcatggtct tcggcttgtc cacggtgacc agcctggcgg ggtcgtccct
     2461 ggtcaaaggg gccgtggcta ccctcctcgg gttgatgctg gcgaccatgg gcgtcgatct
     2521 tcccaccggg atcaaccgtt atactttcgg gatcgataaa ttaatggagg gcttcgacat
     2581 tatcgtggcg gccatcggcc tctttgccgt ttcggagatc ttcatgtccc tggaagagat
     2641 agtcgcggga accctagagc aaatcaaagt cacccggatc tggctttcca tgaaagattt
     2701 cctcgacagc ctcggcgcca ttctgcgcgg gacgggtctg ggctttgtgg tgggggcgat
     2761 tccgggagcc gctcatgtga ccgcttcttt tttctcctat acgctggaaa agaaattcag
     2821 caagaatcct gaaaatttcg gcaagggaga gatccgcggg gtggcgggtc cggaatcggc
     2881 caacaacgcg gctgcttccg gagccctttt gcccctgttg actctggggc tgccggcaac
     2941 cacggttaca gccgtactct acggagccct cctcatggtg ggggtgaaac ccagcccctt
     3001 tctcttccag aagaacccgg atgtcgtctg gggtttagta gccagcatgt atgtgggcaa
     3061 catcatgctc ttgatcctca acctgccgtt ggttggagtc ttcgtgcgga ttctttccat
     3121 ccctacccgg tggcttcttc ccattgtcct ggccatctct tttgtcggcg tttacagcgt
     3181 ccacaattcg gcactagacc tggtgctggc cagcattctc ggattcgtcg gttatttgat
     3241 gcgtaagttc gattttccct tggctcccgt gatcctggga ctagtcctcg gagatttgat
     3301 ggaacagtcc gtccgccagg ccctcatgat ttcaggggga aatgccgcta tcctcttcca
     3361 gagcgccatc gccaagacgc ttttcgcctt ggccgttttt gtggtggtcg ggccgatgct
     3421 gatgcaaaaa atgcggaaga aggagccgta agccgagccc ggaattcgtc gctccctccg
     3481 ccttcctatc aaggctccca gcctgccgtt gggtctctca actgtattga ccgctcccga
     3541 tcgccatgat atgaatccag tatagaagca aaaggtttaa gaaaaccgga ggcggaattg
     3601 aaagagcacc tggccaaact actcgaagaa ctttccctgc tggatggact tcccgggcag
     3661 gagcagcctg tcgtccgata cctaaaggaa aagctgcaac ctcttgcgga ggaagtgcac
     3721 atcggagtga atggaaacat ctatgcccgg aagacagggc gcaaaccggg cttgaaggtc
     3781 ttggtgtca
//
LOCUS       DASPRR010000285         7889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1190761, whole genome shotgun sequence.
ACCESSION   DASPRR010000285 DASPRR010000000
VERSION     DASPRR010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190761"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..1838
                     /locus_tag="VEL68_10960"
     CDS             363..1838
                     /locus_tag="VEL68_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ36528.1"
                     /translation="MDTRNRQEEHGVMPTLDKVFCPQGVAVVGASPVNLSFAALVLHS
                     LKEAKFPAVFPVNPKYKEVLGLPCYPSLTDIPEAVDHVIVSIPAESVLKLLDDCAAKR
                     VKSVHFFTAGFGETGHIEGEELERAMLVKAKAGGFRIIGPNCVGLYVPKSRLVNSINS
                     PLEPGPIAFLSQSGGHAQNLPDYSRARGLRFSKVVSYGNALDVNESELLDYFSRDPET
                     EIIGSYIEGVKDGKRFMKVMAETAAQKPVVIYKGGRSEAGRRAAYGHTASLTSSVAIF
                     DALCRQVKAIQVDDLDEMIDVLTALRFVRPLPRDTGAALMGAGGGPSVLASDEMEKEG
                     LRVPCLPPAVQAELKQSLPVAGSIFINPLDTPNLIAPEAISTAMQVLGRVPEIHMLIY
                     HLGFHPISRWGLGRLSLENFVQPAIAVMKEALRKTGKPVLLALRPPQGLDGMTEFLAA
                     QQEFVKEGFPVFYSMRQLARAMVRVIAWNGPGPDSRGPEEN"
     gene            2150..3361
                     /locus_tag="VEL68_10965"
     CDS             2150..3361
                     /locus_tag="VEL68_10965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36529.1"
                     /translation="MTKKIFYGWWIVLACFIIGLYVAGITFYGFTAFFEPLIKEFGWS
                     YTQVSFAASLRGMEMGVFAPLIGLFVDRLGSRKIIFGGVITVGFGLFLLSFTQSLAMF
                     YAAFFLLAFGAGGCTSVVSMAVVANWFDKNVGKALGVMASGFGASGLMVPIIVWLMEV
                     YQWRSTLVILGLGTLVLGVPLSFVIRNKPEQYGYFPDGELTRGPILPEEIKSSKDGVS
                     FREVLKNRSFLFLNLTEAIRMMAVSSVATHVMPYFSSVGISRSKAGLVAGAIPLFSII
                     GRLALGWLGDVFEKRYVMASAFGLMSVGMLVFCYVQIEWIIVLFLLLFPPSFGGTMVL
                     RGSILREYFGRDSLGKMLGMVMGSASIGGIIGPTLAGWVFDSWGSYQFIWAAFSAFNA
                     LAIMLVMKIKR"
     gene            3514..3627
                     /locus_tag="VEL68_10970"
                     /pseudo
     CDS             3514..3627
                     /locus_tag="VEL68_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NrdH-redoxin"
     gene            3622..4206
                     /locus_tag="VEL68_10975"
     CDS             3622..4206
                     /locus_tag="VEL68_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXZ36530.1"
                     /translation="MIVGDRAFHGWNPRGLAAFLNTQYLESGPLHPDELAKRLDRILE
                     AAQRAIRQVPQEHLGMKAPDRDRTVKDLAYHLFRLSVAYRDAMEQGFLQQEWITSGAP
                     PGVADGPTIALYGQEVRARLSEWLSQPERCRGTVDTYYGKQTAHELLERTTWHAAQHL
                     RQLYFFLERMGVSPENPLTEEDYKGLPIPKDVWS"
     gene            complement(4316..5437)
                     /locus_tag="VEL68_10980"
     CDS             complement(4316..5437)
                     /locus_tag="VEL68_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36531.1"
                     /translation="MSFSVRLEHVHKIFGKKGKEVLAVHDLSVEIGEREFFTFVGPSG
                     CGKTTTLRMVAGLEMPTSGRIFFGDQDVTDLSPQRRDIAMVFQDIALFPYMTVRENIG
                     YPLKIAGVARAEMDRKVGQVAEILAISDKLSMKPGQLSGGQQQRVAIGRALIKEPKVL
                     LLDEPLSALDARLRVEMRTEILRLHREISATIIYVTHDQVEAMTMSSRLAVMDKAKAV
                     QIDRPKNIFRLPRNQTVATFIGTPAMNIIPAALVSWQGGPAVRFLGGQFRVNPAHYET
                     LKRIDRVDAGIRPQALRLVPPTPGKITGKVFLREPLGLEDEVIIQTPDGTRVKIVTGS
                     GQEFLEGSEVGLDFEAKDLYLFHPESKETLCFGIDSSSP"
     gene            complement(5441..6265)
                     /locus_tag="VEL68_10985"
     CDS             complement(5441..6265)
                     /locus_tag="VEL68_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ36532.1"
                     /translation="MKKAAVQHRTLMIVLAIILLLVAFPFYWMLLSSFTPRTVLFQPP
                     YKFFRLDLSVENYKDLFFATEFLTYLKNSVIAAVGAILLNIFAATLAGYGLTRFTFWG
                     KKQFAQGTLFSYMFPAMLMAIPLYIILSALQMRNTYTGLILAHMAISLPLNIWIMWQY
                     FQILPVSLEEAAWVCGAGRLRALREVCLPSAKPGILSVAIFSFALSWNDFTFAFILQT
                     DKEMFTLPIGLATFVEQTAIHWGMVMSSAVLVSLPTFALVFFLQRYLLSGLRVGGS"
     gene            complement(6262..7158)
                     /locus_tag="VEL68_10990"
     CDS             complement(6262..7158)
                     /locus_tag="VEL68_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ36533.1"
                     /translation="MDKRDSQYWLWVVGTTVPALLFLTIVAYLPILYAVGISFFKKTA
                     FSPAMTWAGLKNYAFILEEPDFWAALVRSIVFTMGSVLVQLIWGVGVALLLNRTFRGM
                     TAIRTLFILPYLLPTIVVALVFQWLLNQEYGVINQILLQTGAVRTPINFVGGLNTAMY
                     SVIGTASWQYGSFVALMVLARLQAIPPRLYEAARVSGAGTIRCFWDVTLPNLKTTIVL
                     LALLRGIWMFNKFDIIWLITRGGPLKATETLPLYAYRLAFEEFDFGVAASACTVMFLV
                     LVVGSIVYFKLFDPTKEVEVGR"
     gene            complement(7434..>7889)
                     /locus_tag="VEL68_10995"
     CDS             complement(7434..>7889)
                     /locus_tag="VEL68_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36534.1"
                     /translation="DATAPVALPKRLTDHYMVSIQGFHILNQSNVEGAKQYVKFFLKH
                     PDCIQWYHAVPLHIIPASREVLRSAKYQDHPVIQKRMDVLNFLDSIWGKGVPLYYWDG
                     KEMNPYIGLYHNENLGGWMVAMRNIKKDKSETIVDEAANKIRSKMKRMG"
BASE COUNT         1709 a         2051 c         2270 g         1859 t
ORIGIN      
        1 tttgactccc acgaaagcta atccaggcct gaaagggcgg ttacgatgag accgtggtgg
       61 gaattggggg acgagggacc attaggcaat ctggccgggt caaaagggtg gtcaagcccc
      121 ctcctggtct tctttgctca aagcccgccg aaatggtttc cacctttcac gttcaggttc
      181 cataacctgg ccataaggca taaatgttct tcccattttc ctctcctgcc gtgcttcaat
      241 ttccttgacc ctcaatggcc ttctcagtat actcacacta aaaaatcgaa ctcttatcag
      301 acccgcaaga attcccttga atatttggct gaaaatcatg tcgtgacctt gaggatgatg
      361 cgatggatac gcgtaatcgg caagaggaac atggagtcat gcccacgctg gacaaggtct
      421 tttgtccgca gggagttgct gttgtgggag cctctccggt caatctttcc tttgcagcat
      481 tggtccttca ttctttgaaa gaggcgaagt tccccgctgt ctttccggtc aatccgaagt
      541 acaaggaagt gcttgggctg ccctgctacc caagcctcac ggacattccc gaagcggtgg
      601 accatgttat cgtgagcatc ccggcagagt cggttcttaa gctcttggat gactgtgcgg
      661 caaagagagt caaatcggtt cacttcttca ccgcggggtt tggggagact ggccacatcg
      721 aaggggaaga actcgaaagg gccatgctgg tcaaggcgaa ggcggggggc ttccggatca
      781 tcggccccaa ttgtgtcggc ctctacgtgc ccaagagtcg gctggtcaat agtataaatt
      841 ctccgctgga acctggtccc atcgcttttc tttctcagag tggaggccat gcgcagaatc
      901 tcccggacta tagccgggca agagggcttc ggtttagcaa ggtggttagc tatggaaatg
      961 ctctggatgt gaatgaaagc gaactcctgg attacttctc cagggatccg gagaccgaaa
     1021 tcatcgggtc ctacattgag ggcgtgaagg atggtaagcg tttcatgaaa gtgatggccg
     1081 agacggccgc tcaaaagccg gtggtcatct acaagggagg cagaagcgag gcaggcaggc
     1141 gggctgcata tggtcatacg gcaagtttga ccagctcagt cgccatcttc gatgccctct
     1201 gccgtcaggt aaaggctatt caggtggatg atctggacga gatgatcgac gtcctcacag
     1261 ccctgcggtt cgtccgccct ctgccccggg ataccggggc tgccctcatg ggcgctggcg
     1321 gaggccccag cgttcttgcc agcgacgaga tggaaaaaga gggccttcgc gtgccgtgtc
     1381 tgccaccggc ggtgcaggcg gaattgaaac agagtcttcc cgtggccggg agcatcttca
     1441 tcaatcccct ggataccccg aacctcatcg ctccagaggc tatttcgaca gccatgcagg
     1501 tgctcggccg ggtcccggag attcacatgc ttatttacca tctcgggttc catccgatca
     1561 gccgttgggg attgggccgg ctatccctgg agaactttgt ccaacccgca atagccgtca
     1621 tgaaggaagc cctgcggaaa accggcaagc ctgttcttct tgccctgcgt cctccccagg
     1681 gtcttgatgg aatgacggaa ttccttgcgg cccagcaaga atttgtaaaa gaaggcttcc
     1741 cggttttcta ttccatgcgt cagttggccc gggccatggt tcgggtcatc gcgtggaacg
     1801 gacccggtcc tgattccagg ggtccagaag agaactgaca aacgattcga tgccaccgta
     1861 tcaagtacgc atatatttct cctcaaaacg ccttgatcct tctcgaatga cctaagggca
     1921 atctgagcag tttttgatga tctctccctc gccattggat tcattgcctg gatgcgaccc
     1981 ttctaccttg atgaaccaat ccattcccat ttttgttacc tccagcctct aaaagggggc
     2041 ctctttgctt taagagaaag aggtcatcct aaacctcatt tacagggagg tgacgccatg
     2101 gtatatatcc ataaaattct tcgaatttct ttcattgaaa tcttttcaga tgaccaaaaa
     2161 gatcttttac ggatggtgga ttgtccttgc ctgtttcatt attggcctct acgttgccgg
     2221 aattaccttc tatggtttta ctgccttttt tgagccgcta ataaaggaat ttggctggag
     2281 ctatacccag gtctcgtttg ctgccagctt acgcggtatg gaaatgggag tgttcgctcc
     2341 actaatcggt ttgttcgttg atcgcttggg gtcaagaaag ataatttttg ggggagtgat
     2401 cacagttggc tttggcctct tcctcctcag ttttacccag tccctggcca tgttttatgc
     2461 cgcgtttttt ctcctggctt tcggcgccgg aggatgcacc agcgtagtta gcatggctgt
     2521 ggtggctaac tggtttgata agaatgtggg gaaggccttg ggggtgatgg cttctggatt
     2581 tggcgccagt ggtttaatgg tgcctatcat tgtttggctg atggaggttt atcaatggcg
     2641 gtcaaccttg gttattcttg gtttggggac tttggttctg ggggtccctt tatcctttgt
     2701 catccgtaac aaaccggaac aatacgggta ctttcctgat ggggaattaa caagggggcc
     2761 gattcttcca gaggaaataa agtcgtcaaa agacggcgtt agcttcaggg aggtgcttaa
     2821 gaatcggtcc ttcttgtttt tgaatttgac cgaagccata cggatgatgg ctgtttcttc
     2881 tgtggccacc catgtcatgc cctatttttc gagtgtcgga atatcaagat caaaggctgg
     2941 cctagtggcg ggggccattc ctctatttag catcattggc aggctggccc ttggttggct
     3001 gggagatgtt ttcgaaaaga ggtatgtgat ggcctcggca ttcggcctta tgagcgtggg
     3061 aatgctggtt ttttgttatg ttcaaattga atggattatt gtcctttttc ttttgctctt
     3121 ccctccgagt tttggcggca caatggtcct gagggggtct attttgaggg aatattttgg
     3181 gagggattct ctgggcaaaa tgcttgggat ggttatgggc tctgcatcca ttggagggat
     3241 tattggtcct acccttgcgg gatgggtatt tgattcttgg gggagctatc aatttatttg
     3301 ggccgccttt tccgccttta atgccttagc aataatgctg gtaatgaaaa taaaacgtta
     3361 agcctttttg ccttcctttt aacctcggat tgtacagaat ctgttgagtc tttgcctcgg
     3421 aaacccgata tcttaaaata ctccttccct ttggaggtgt ttatggttcc acgcgtgtac
     3481 taccgtccag gttgaagctc ttgcgccaag acgaaagagt tgctttcgtc atgggggatt
     3541 gcctatgaac cggttaatat tgaagcacag ccagaagcaa tgctggacct gaagttgcat
     3601 gggatccgat cggttccagc ggtgatcgtt ggcgaccggg cttttcatgg ctggaatccg
     3661 cgggggctgg cggccttcct aaatacccaa tatttggaat ctggccctct ccatcctgat
     3721 gagctggcga aacgtcttga ccgtatcttg gaggctgctc agcgggccat ccgccaggtc
     3781 ccccaagaac accttggcat gaaggcgccg gatcgcgacc gtacggtaaa ggaccttgcc
     3841 taccatctct tccgcctcag tgttgcctat cgggatgcaa tggaacaggg atttttgcag
     3901 caggagtgga tcactagtgg agccccgcct ggtgttgccg atggaccgac gatcgccctc
     3961 tacggccagg aggtccgggc gcgattatcg gaatggctga gtcagccaga gaggtgccgg
     4021 ggaacggtgg atacttatta tggcaaacag accgctcatg agcttttgga gcgtacaact
     4081 tggcatgcag cccagcatct tcgccagctc tatttcttcc ttgagcgcat gggagtcagc
     4141 cccgagaatc ccttaaccga ggaggactat aaagggctgc cgatcccgaa ggatgtttgg
     4201 tcttagattt tgagacattt cgatcaaggg taagggtgcc taggccacct tagctgcgga
     4261 aggataatcc cgcatatttc actcgtacgg gttgaggcat cttattgatt taggtttagg
     4321 gggaagagga gtcgatcccg aagcagaggg tctccttcga ttcggggtgg aagagataaa
     4381 ggtccttggc ttcaaaatcc agtcccacct cgctgccttc aagaaattcc tgcccactcc
     4441 cggtgacgat tttgacccgc gttccatcgg gggtctggat aatgacctcg tcttccagcc
     4501 ccaggggttc acgcagaaag accttcccgg tgatcttgcc gggcgttgga ggaaccaggc
     4561 ggagggcctg ggggcggatt cccgcatcca cccggtcgat tctcttcagc gtctcgtagt
     4621 gggcgggatt aacccggaac tgccccccca gaaagcgaac cgccggcccg ccctgccaag
     4681 agaccagagc ggccgggatg atgttcatgg ccggggtacc gataaaagtg gccacggtct
     4741 gattccgggg aagtctgaag atgtttttgg gccggtcgat ctgaaccgcc ttcgctttat
     4801 ccatgaccgc gaggcgggaa gacatggtca tggcctccac ttggtcgtga gtcacgtaga
     4861 tgatggtcgc cgaaatctcc cggtgcaggc ggaggatctc ggtgcgcatc tccacgcgca
     4921 gccgggcgtc gagggctgac agaggctcgt ccaaaagcag gaccttgggc tccttgatca
     4981 gggcccggcc gatggccacc cgctgctgct gtcctccgga gagctggccc ggcttcatgg
     5041 agagcttgtc agagatggcc agaatctccg ccacctgccc caccttacgg tccatctccg
     5101 cgcgcgcgac tcccgctatt ttcaggggat acccgatgtt ctcccggacg gtcatataag
     5161 gaaataaagc gatatcttgg aagaccatgg caatgtcccg cctctgcggg gaaaggtcgg
     5221 tgacgtcttg atccccgaag aaaatccgcc cggaggtggg catctccagc cccgcgacca
     5281 tgcgcagggt cgtcgtcttg ccgcatccgg agggccccac gaaggtgaag aactcgcgtt
     5341 cgccgatctc caccgaaagg tcgtgcacgg caaggacttc tttccccttc ttgccgaaaa
     5401 tcttatgcac atgttcgagg cgaaccgaaa aagacatatc tcaacttcct cccacccgca
     5461 atccgctcag gagatacctc tgcaggaaga acaccagggc aaaggtcggc agggacacga
     5521 gaaccgccga agacatgacc atgccccaat ggatggcggt ctgctccacg aaggtggcca
     5581 ggccgatggg caacgtaaac atctccttgt ccgtctgcaa aataaaggcg aaggtgaagt
     5641 cattccagga gagggcgaag gaaaagatgg ccaccgaaag gattccgggt ttggccgagg
     5701 gcaagcagac ctcccgcagg gccctcaggc ggccggcccc gcagacccag gctgcttcct
     5761 cgagcgatac gggaaggatt tgaaaatatt gccacatgat ccagatgttc aggggaaggc
     5821 tgatggccat gtgggccagg atcaggccgg tgtacgtatt gcgcatctgg agggccgaaa
     5881 ggatgatgta caggggaatg gccatcagca tggccggaaa catgtacgag aagagcgttc
     5941 cttgggcgaa ctgcttcttc ccccagaagg taaaccgggt cagcccatag ccggccagcg
     6001 tagccgcgaa gatattcagg aggatggcgc ccaccgcagc gatgacgctg ttcttcaggt
     6061 aggttagaaa ttcggtggca aagaagaggt ccttgtaatt ctccaccgaa aggtcgaggc
     6121 ggaagaattt ataggggggc tggaaaagca cggtccgggg cgtaaaggaa ctcagaagca
     6181 tccagtagaa gggaaaagcg actaggagca gaatgatcgc caggacgatc atcagggttc
     6241 ggtgttggac cgcggccttt ttcatcttcc cacttccacc tctttggtgg gatcgaagag
     6301 cttgaaatag acgatcgaac ccaccaccag aaccaggaac atgaccgtgc aggcgctggc
     6361 cgcaaccccg aaatcgaact cctcgaaggc cagccggtag gcgtagaggg gcagcgtttc
     6421 cgtggccttc aagggtcctc cccgggtgat cagccagatg atgtcgaact tgttgaacat
     6481 ccagatgcct cggagcaggg ccagcagtac gatggtggtc ttcagattgg gcagggtcac
     6541 atcccagaag cagcgtatcg ttccggcccc gctgacccgg gccgcttcgt agagcctcgg
     6601 ggggatggcc tgcagacggg ccagcaccat gagggccacg aagctgccat actgccagct
     6661 cgccgtgccg atgaccgaat acatggccgt gttcagtccc cccacgaagt taatgggagt
     6721 ccggacggcg ccggtctgca ggagaatctg gttgatgacc ccgtactcct ggttcagcag
     6781 ccactgaaag accagggcga ccacgatcgt ggggaggagg taggggagaa tgaacagggt
     6841 gcggatggcg gtcatccccc ggaaggtccg gttcagaagc agggccactc ccactcccca
     6901 gatgagctga accaggaccg agcccatggt gaagacgatg gagcggacaa gggcggccca
     6961 gaaatcaggc tcctccagga tgaaggcata attcttgagg ccagcccagg tcatggcggg
     7021 ggaaaaggcg gtcttcttga aaaagctgat gcctaccgca tagagaatgg gaaggtaagc
     7081 cacgatggtg aggaagagga gggccgggac tgtggttccc accacccaca gccagtattg
     7141 agaatctctc ttatccatga acccctacga atcccgttaa aaaaatccct cccccttcag
     7201 aggctgtgtc gtaatccgtt catggttcga caagctcacc acgaacggat tatgagtgta
     7261 ttaaaattca gcaaattagc cgttcgccct gagcctgtcg aagggcgacc gggagattac
     7321 gacacagcct ctcaacgggg gagggttggg gtgccatgaa ctacaggctt tagtgccgat
     7381 gctctatgct ccatgcgttt tgttcctggg agcttaccct acagctcacg cctttacccc
     7441 atccttttca tcttcgagcg gatcttattg gccgcctcgt cgacgatggt ctcggattta
     7501 tccttcttga tgttcctcat ggccaccatc cagccgccca ggttctcgtt gtggtagaga
     7561 ccgatgtagg ggttcatctc cttgccgtcc caatagtaca ggggaactcc cttgccccag
     7621 atggaatcga ggaaattgag tacgtccatc cttttttgga tgacgggatg atcctggtat
     7681 ttggccgaac ggagcacctc ccggctggcc ggaatgatgt gcaggggaac ggcatggtac
     7741 cactggatgc agtcggggtg tttgaggaaa aacttcacgt attgcttcgc tccctccacg
     7801 ttgctctggt tcaggatgtg aaacccctgg atggagacca tatagtggtc cgtgagcctc
     7861 ttgggcaagg ccacgggcgc cgtggcatc
//
LOCUS       DASPRR010000286         3887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2001576, whole genome shotgun sequence.
ACCESSION   DASPRR010000286 DASPRR010000000
VERSION     DASPRR010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001576"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VEL68_11000"
     CDS             <1..197
                     /locus_tag="VEL68_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ36535.1"
                     /translation="LGHPGGAIYGFDQHAKDSYFFEDRRSPVQGLYFAGSWVAPGGYQ
                     TTLQSGVSAARAILKSMSKR"
     gene            206..1366
                     /locus_tag="VEL68_11005"
     CDS             206..1366
                     /locus_tag="VEL68_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017353500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXZ36536.1"
                     /translation="MSRDFRPEIEGYGEIRREIEILRRYGLDYSSHKGRVGQVLNLLH
                     PKRIGLKVSEVRKETQSAKSFRLVSPDGYLPPFQAGQYINLFVDVGGVRTSRPYSISS
                     PPNQNGYYEIAVRRVEDGFVSSHLLETVKVGDRLESTGPTGNFFHNPLFHGNDLVFLA
                     GGSGITPFMSMIREVTDRGLSRKIHLIYGSRNPDDMIFGEELEERASLHENFRLTSVI
                     SEPSAGFEGFTGFITARLMKDVLGSVGGKTFYVCGPEAMYTFCLPELLKLQVPARKIR
                     MEVSGPPKDVTAQPGWPEMVGADSQFQVTVKSKKRIDARAGEPLMISLERAGIAIPAS
                     CRSGECSLCRTKLLSGKVFQPPGAKLRKSDRTYGYIHPCVAYPLQDLEIMIF"
     gene            1464..1940
                     /locus_tag="VEL68_11010"
     CDS             1464..1940
                     /locus_tag="VEL68_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HXZ36537.1"
                     /translation="MKKIENQLIGICGLYCKTCPRYLAYQDQDIEELKKMSQADGVPV
                     EEIRCDGCLSDHVYPTCRDCRHGFRRCAEEKKVTWCFQCPDFPCKRLEDFSKVHVVNG
                     IWHHKKVIDHLQFMRDEGIEEWVKEQEKEGACPNCGKMLYWYALDCARCGISVRPK"
     gene            complement(2001..2849)
                     /locus_tag="VEL68_11015"
     CDS             complement(2001..2849)
                     /locus_tag="VEL68_11015"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HXZ36538.1"
                     /translation="MESQNKSILITAAVLVLLLLGAGVLYWYYTRPPATPPAIEQPKA
                     EAPSQPPPSAEVKVGEEKEAPPQPEVKIPALDQSDDFVRQMMKNLSPHGKLGEWLKIK
                     NIIRVFVAAVDNIANGKSPRPQLGFLSPGQAFPVGEKGDRIYLDPKGYERFDIITDAI
                     VSFSSSRTVQGYQKLRPLFQEAYRELGYPQKDFHATFIQAMKRIMDTPIVEREVLLKE
                     EGKGLNYVFIDDGLENMSEVQKHLLRMGPKNTKKIQQKVREIALALGVPETQLPQAQI
                     YIPRAQ"
     gene            complement(2925..3812)
                     /locus_tag="VEL68_11020"
     CDS             complement(2925..3812)
                     /locus_tag="VEL68_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ36539.1"
                     /translation="MEERVITRRDFLRGAAGTALAAAMGGGTAEKAEAQPRAKVVLIR
                     QAEVLGRHDKVQAEVVQSMLDEAVRTLLGESDILTAWKKLIQRSDVVGIKSNVWWNLP
                     TPGELEGAIKRRVLEVGVPEKNISIDDRGVLNNPVFQKSTALINVRPLRTHHWSGVGT
                     CLKNYIMFVPDPDSYHDEGCSPLGKIWQSPLVNGKTRLNILCAFTPQFYGRGAHYFDK
                     RYVWPYKGLIVGTDPVAVDAVGAHLLQIKRVAFFGEDRALDAPPVHIMAADKKYNLGV
                     SELNKIQLIKLGWQGEVLI"
BASE COUNT          879 a         1048 c         1043 g          917 t
ORIGIN      
        1 acctcggcca tccgggaggg gccatctacg ggttcgacca acatgccaag gactcctatt
       61 ttttcgaaga ccgccgctcg cccgttcagg gactttactt tgccggctcc tgggtggccc
      121 cgggcgggta ccagaccacg cttcagtcgg gcgtctccgc cgcccgggcc attctgaaat
      181 ccatgagcaa gagatgaggt gaggtatgag ccgagatttc agaccggaaa tcgaagggta
      241 tggagagatt cggcgggaga tcgaaattct gcgaaggtac gggttggact attcatccca
      301 caaaggaaga gtgggacagg ttctcaatct cctccatcct aaaagaattg gcttgaaggt
      361 ttccgaagtc cggaaagaga cccaaagtgc caagtcattt cgtttggtct ccccggatgg
      421 ctatcttccc cccttccagg ccgggcaata catcaatctt tttgtcgatg tgggtggagt
      481 caggaccagc aggccttaca gtatttcttc cccacccaac caaaacggat actatgaaat
      541 tgcagtccgg cgggtggagg acgggtttgt atccagccat ttgctggaga ccgtgaaggt
      601 gggcgaccgg ttggaaagta ctggcccaac agggaatttc tttcacaacc ccctcttcca
      661 cggcaacgac ctggtgttcc tggccggggg cagcggaatc actcctttta tgagcatgat
      721 ccgggaagtc acggatcgcg gcctttccag gaaaattcat ctcatctatg ggagtcggaa
      781 cccagatgac atgatctttg gagaagagct tgaagaaagg gccagcctcc acgaaaattt
      841 tagactgacc tctgttatct ctgaaccctc ggcaggattt gagggtttca ccggctttat
      901 caccgcaaga ctcatgaagg atgttctagg cagcgtggga ggaaagactt tctacgtctg
      961 cgggcctgaa gcgatgtaca ccttttgcct cccggaactg ctgaagctcc aagtcccagc
     1021 ccggaaaatc cggatggagg tctccggccc gcccaaagac gtaaccgctc agcccggatg
     1081 gccggagatg gtgggtgcgg acagccaatt tcaagtgacg gtgaaaagca agaagaggat
     1141 cgatgcccgg gccggagaac ctctgatgat atccctggaa agagcgggca tcgcgattcc
     1201 tgcttcctgc cgctcgggag agtgcagcct ctgccgcacc aagctccttt cgggaaaggt
     1261 cttccagccg ccgggggcca agctgaggaa atccgaccgg acctacggat acatccaccc
     1321 ctgcgtcgcc tatcccctgc aggacctgga gatcatgata ttttgattgt ggaattcgga
     1381 attcgaattc cgctctgagc cgtgtgaggt tcagggcagc cctgcattct atttagcgca
     1441 gggctggaat gaaaagggaa cccatgaaaa agatagagaa tcaattgatc ggaatatgcg
     1501 ggctttactg taagacctgc ccgagatacc ttgcctacca agaccaggat atcgaagaat
     1561 taaagaaaat gtcccaggcc gatggggtcc ccgtcgagga aattcgctgc gacggctgcc
     1621 tttccgacca tgtctatccc acctgccgcg attgccggca cggttttcgc cgatgcgccg
     1681 aggaaaagaa agtgacctgg tgcttccagt gccctgactt cccctgtaaa cggctggagg
     1741 atttttccaa ggtccatgtg gtaaacggca tttggcacca taaaaaggtg atcgatcatc
     1801 tccagtttat gagggatgag gggatcgagg aatgggtaaa agagcaggag aaagagggtg
     1861 cctgcccgaa ttgcgggaag atgctctact ggtacgctct ggattgcgcc cgctgcggca
     1921 tctcggtgcg tcccaaatag gcatttgtcc ccgaaattta cataggcaaa ccaatacgaa
     1981 agacaaaatt gtcatcaccc ttattgcgcc cggggaatat agatttgcgc ctgggggagc
     2041 tgggtttccg gtacgcccaa ggccagggcg atctcgcgta ccttttgttg aattttttta
     2101 gtgttcttcg gccccatgcg caggagatgt ttctgaactt cactcatgtt ttccaagccg
     2161 tcgtcgataa aaacatagtt gagccccttc ccttcctctt ttaaaaggac ctctctttct
     2221 acaatcggcg tatccatgat tcttttcatc gcctggataa aagtcgcatg gaaatctttc
     2281 tgcggatacc ccaattcccg gtaggcctcc tggaagagag gtcttagctt ttggtagccc
     2341 tggacagtcc gcgaggaact gaaagaaaca atcgcatcgg taataatatc gaacctttcg
     2401 taccctttgg ggtccaagta gatgcggtcc cccttttcgc caaccggaaa ggcttgcccg
     2461 ggtgacaaaa agccaagctg gggcctgggg cttttgccat tggcgatatt atccactgct
     2521 gctacaaaaa cccggatgat attcttgatc ttcaaccatt cgcccaattt tccatgaggg
     2581 gatagattct tcatcatttg gcggacaaag tcatcgctct gatcgagggc cggaattttg
     2641 acctccggct ggggaggggc ctccttttct tccccaacct tgacctccgc agaaggaggg
     2701 ggctgagagg gtgcctcggc ttttggttgt tcaatagctg ggggagtcgc agggggacgc
     2761 gtgtaatacc aatacaaaac cccggcaccc aggagaagga ggaccaacac cgctgcagtg
     2821 atcaggatgg acttgttttg actttccatt attctcaacc tcctcggtta ggttttcgaa
     2881 ggccggttga tcgaatggaa ggccggcagg ctcaagaata ttggttaaat taaaacctct
     2941 ccctgccagc ctagcttgat gagctgaatt ttatttaact cgctgactcc gaggttatat
     3001 ttcttatccg ccgccatgat atgaacggga ggagcgtcca gagcccggtc ttcgccgaag
     3061 aaagcgaccc gcttaatctg caggagatgg gcacccacgg cgtcaacggc taccgggtcc
     3121 gtgccgacaa tcagcccttt atagggccag acataccttt tgtcaaaata atgggctccc
     3181 cttccgtaga attggggagt gaaagcacaa aggatattca ggcgggtctt tccgttcacc
     3241 aggggagatt gccagatttt gcccaggggg gaacatccct catcgtggta ggagtcagga
     3301 tcagggacga acataatgta attcttcagg caggttccca cccccgacca gtgatgggtt
     3361 ctcagaggcc tcacattgat cagggccgtt gacttttgga agacggggtt gttcagaacg
     3421 ccccggtcat cgatagaaat atttttctcg ggcacgccca cttccagaac ccgccttttg
     3481 attgccccct ccagttctcc gggagtcggc aggttccacc agacgttgct cttgatcccc
     3541 accacatccg atctctggat aagcttcttc catgcggtta gaatgtcgct ttctcccagg
     3601 agagttctga ccgcctcgtc cagcatggat tgcacgacct cggcctgaac tttatcatgc
     3661 cgtcccagaa cttcggcctg gcggatgaga accacctttg ctcggggctg ggcttcagcc
     3721 ttttctgctg ttccccctcc catcgccgcg gccagggctg ttcccgctgc ccctctgagg
     3781 aagtccctcc tggtgataac cctttcttcc atttttcgat gttaaattcc ccctccgccc
     3841 ccctttttca aaggagggaa cggggagatt tctacggatc ccttgac
//
LOCUS       DASPRR010000287         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1842081, whole genome shotgun sequence.
ACCESSION   DASPRR010000287 DASPRR010000000
VERSION     DASPRR010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842081"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VEL68_11025"
     CDS             <1..982
                     /locus_tag="VEL68_11025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXZ36540.1"
                     /translation="DEIILPALSFVATGNAVLHAGFKPVFVDIDRKTLNIDVNQIEKA
                     ITERTRAIMPVHLMGKPAAMDPLLAIARKHGLYVVEDAAEAHGAEYRGKPVGALADMA
                     AFSLYVAHIISTVEGGIVTTDREDFAEVLRSLRSHGRACKCRQCVLNSTQAHCPKRFN
                     GGNGEDMRFVFERIGYSTKMNELEAAIGLSNMEIYREILDKRRRNLLYVLERFSRFHP
                     YLATIQEEAHEKIGPHAIPIVVQEAASFSRAELAHYLETNGIETRTLFASMPTQCAGF
                     AWLGYREGQFPQAEYLGKKGIHIGVHQDLGLEEMDFVLQTLEKFLNTYQR"
     gene            998..>2373
                     /locus_tag="VEL68_11030"
     CDS             998..>2373
                     /locus_tag="VEL68_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ36541.1"
                     /translation="MKQTDIVLVKPGNQKAVYGELSSFNLTAFEPPFWCALLAAYLRD
                     SGYSVEVFDSEIENWSYEETAQRMQEVKPLLAAVIVSGHNPSASTMNMTGAGKILRHL
                     KERTPDIKTLLAGLHPSALPERTLREEAVDFLCQGEGFYTFPQLIEGLKAEREDFPIP
                     GLWYKRAGRIISNPRPPLMEDLDRLPMPAWDLLPLAKYRAHNWHCFDRIHARQPYAVI
                     YTSLGCPFRCSFCCINSLFGKAGIRYRSPERVVEEIDYLVKNHNLRNIKIIDEMFVLK
                     ESHVVRLCDLIIERGYDLNIWAYARVNTVNERMLRKMKQAGIRWLAYGFESGNKKVLQ
                     AVTKGYDLDAVGRVVQMTRDAGIYICGNYIFGLPDDDASSMQETLDLALEINAEWANF
                     YGTMAYPGSQLYEQAIAGGWPLPETWQGYSPYAYETLPLPTKYLTGPEVLRFRDEAFK
                     TYFTRPSYL"
BASE COUNT          549 a          665 c          641 g          518 t
ORIGIN      
        1 ggatgagatc atcctgccgg ccttgtcctt cgtcgccacg ggaaacgcgg tcctccacgc
       61 cgggttcaag cccgttttcg tggacatcga ccggaagacc ctgaacatcg atgtgaacca
      121 gattgaaaaa gccatcaccg aaaggacgcg ggccattatg cccgtccacc taatggggaa
      181 accagcggcc atggacccgc ttctagccat cgcccgcaag cacggattgt atgtggtgga
      241 agatgcagcc gaggctcacg gagccgaata taggggcaaa ccggtagggg ccctggcgga
      301 tatggctgct ttcagcctct acgtagccca tatcatttcc acagtagagg gcggaatcgt
      361 aaccactgac cgggaggatt tcgctgaagt tctccgttcc ctccggtctc acggccgagc
      421 ctgcaaatgc cggcagtgtg tcctaaacag tacccaggcc cactgcccca agaggttcaa
      481 tgggggcaat ggggaggaca tgcggtttgt cttcgagcgg atcggctatt ccaccaagat
      541 gaacgagctc gaagcggcca tcggcctgag caacatggag atctaccggg agattctgga
      601 taaacgccgg cggaacctgc tctatgttct ggagcgtttc tcccggttcc atccctacct
      661 ggccactatt caggaagaag cccacgagaa aatcgggccc catgccatcc ccatcgtggt
      721 ccaggaggcg gcctccttca gccgggccga acttgcccac tacctggaaa cgaacgggat
      781 tgagactcgg accctttttg cctccatgcc cacccagtgt gcggggttcg cctggctggg
      841 ctaccgggaa ggacagttcc cccaagccga atacctgggg aagaaaggga tccacatcgg
      901 ggtccaccag gacctgggcc ttgaagagat ggattttgtt ttgcagaccc tggagaaatt
      961 cctaaacacc tatcaaaggt aagaagatcg ggatccgatg aaacaaaccg acatcgtctt
     1021 agtaaaacct ggaaatcaga aagcggttta tggcgagctg agttccttca acctcacggc
     1081 cttcgagccg cccttctggt gcgctctgct ggcagcctat ctgagggatt caggatattc
     1141 cgtagaggta ttcgattccg agatagaaaa ctggtcatat gaagagaccg cgcagagaat
     1201 gcaagaggtt aaacctcttt tggctgcggt catcgtttcg ggacacaatc cctccgcctc
     1261 gaccatgaat atgaccggtg ccgggaagat tcttcggcac ctcaaagaaa ggacccctga
     1321 cattaagact cttttagctg gacttcatcc ttcagccctc cctgagcgca ccttgcgtga
     1381 ggaggcagtt gatttccttt gccaaggaga gggtttctac acctttcctc aactcataga
     1441 gggtctaaag gcggaaaggg aagattttcc catccctggc ctctggtaca aaagggctgg
     1501 acggatcatc tccaaccctc ggcccccctt gatggaggat ctggatcggc ttcctatgcc
     1561 cgcctgggat cttctccccc tggctaaata tcgagctcac aactggcatt gctttgatcg
     1621 cattcacgcc cggcaaccgt acgccgtaat ctatacaagt ctggggtgtc ctttccggtg
     1681 cagtttttgc tgcatcaact ccctgttcgg aaaggccgga atccgctatc gaagcccgga
     1741 gcgggtagtg gaagagattg attatctggt gaagaaccat aatctgagaa atatcaaaat
     1801 catcgatgaa atgttcgtcc tgaaagaatc gcacgtggtc cgcctctgcg acttaattat
     1861 cgagcggggg tacgatttga atatctgggc ctatgcccgg gtcaacacgg tgaacgaaag
     1921 gatgctacgc aagatgaaac aggcaggcat ccgttggctg gcttacgggt tcgaatcggg
     1981 caacaagaaa gtcctccagg ctgtgaccaa agggtatgac ctggacgctg tgggccgggt
     2041 ggtccagatg acccgggacg ccggcattta tatctgtggc aactatatct ttggccttcc
     2101 cgacgatgac gcttcgtcca tgcaggagac cctggacctg gctttggaaa tcaatgccga
     2161 atgggccaat ttttatggaa ccatggccta ccctggctcc cagctctatg agcaggccat
     2221 tgctggaggc tggccgctac cagaaacctg gcagggctat tccccctatg cctacgaaac
     2281 attgcccttg cccacaaaat atttgactgg ccccgaagtt ttgcgcttcc gggacgaggc
     2341 ctttaagacc tatttcaccc gccctagcta tct
//
LOCUS       DASPRR010000288         5251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1356353, whole genome shotgun sequence.
ACCESSION   DASPRR010000288 DASPRR010000000
VERSION     DASPRR010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356353"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VEL68_11035"
     CDS             complement(<1..888)
                     /locus_tag="VEL68_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXZ36542.1"
                     /translation="MIEAGRGLGKRLLLWIGRWTAVGIIGAGWMSPALSATKVLIGKH
                     TIVYDIVNPTGVEFLPNYSNSSYSQKVLQEDEFSKRVLIEVNLAPLNSTAPFPISTQQ
                     IPYQMKSFLGPEKDIPVNEGNILSQAEALAQGSRTVQEAVTSIFNWIVDNFTYDAGRG
                     TPQDGRSVFYSKRGSCVGYTNLSIAMLRSLGIPARYAHGYLPPGYDWGISKKYWGVQI
                     SGGGYHAWVEVYYPDVGWCFSDLLHSKNFVDPFHVLRYTDGVNLNPRNIRGGSLDVED
                     ATTFTIFQEENATLAVDQLP"
     gene            complement(881..1978)
                     /locus_tag="VEL68_11040"
     CDS             complement(881..1978)
                     /locus_tag="VEL68_11040"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HXZ36543.1"
                     /translation="MRKMGFSFWSVFLVLILSSPLSMAQDRREVVENLPSGAINWSQG
                     AVTAKGSGAPPKGITNQAQARLMAERAAKADALRNLLETVKGVQVDSETTVESFTTKS
                     DRVLTRVRGMVIGARVVDTKYLSDGAVEVTVTINMTGELLAVMLQELPPLSQPAMPSP
                     PLPQPLPPPVIPSLGPPPVPKPVPPPPPPPAPPKPTVQDTKLPPPPSPPQETKVQPPP
                     VAEPSGTPPPSIEPKVQTPAKAPAKAEENLDLKKLDYTGLIIDARKLGLRPALVPKIL
                     NEKGELVYSSQSVGQQDIARMGLVGYAKDVNAAARNQRVTADPVVVNGLNATGEKKTD
                     VVISNRDAQVVLTTAPYTGYLKNGRVMVVYD"
     gene            complement(2525..3208)
                     /locus_tag="VEL68_11045"
     CDS             complement(2525..3208)
                     /locus_tag="VEL68_11045"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXZ36544.1"
                     /translation="MAVLWKKGKKGVPRDYQRERLIMVDEQLRRRGISDRRTLEAMAK
                     IPRHLFVLEDYRDSAYEDRPLPIGEGQTISQPYMVAIMTQSLELKGQERVLEIGTGSG
                     YQTAILAELAQKVFTIERIPALTERARKVLGDLGYSNISFFSGDGSRGWPEEAPFDGI
                     MVTAGSPDVPPTLKSQLAEGGRLVIPTGPRFTQTLYKLTREGDQFSEEDVTGCVFVPL
                     VGDYGWKDE"
     gene            complement(3457..4665)
                     /locus_tag="VEL68_11050"
     CDS             complement(3457..4665)
                     /locus_tag="VEL68_11050"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXZ36545.1"
                     /translation="MDPNLVSELLISGSSKIVFLIMDGLGGIQMEGKGGTELQVARTP
                     NLDKLAEASSCGLLDPVAPGITPGSGPAHFALFGYDPVQYNIGRGILEATGIDFPLTE
                     KDVVARMNFATVDSGGKVTDRRAGRISTETNQRVCQKIRQGLKPGAGLEVIVQTVKEH
                     RGILVLRGEGLRGDIQDTDPEREGAPPLEPKALVPEAQETAGRVRDILSQVGRILADE
                     PKANMVLLRGFAKHTRYPSMKERYGLNALAIAAYPMYRGISRLVGMTVLSQVASLEEE
                     FKALEENYPRYDFFFLHVKQTDSRGEDGNFDAKVSVIEEVDGYLPRVRALNPDVLVVT
                     GDHATPAKLASHSWHPLPVILHSQYCLVDGVRKFDEVSCIQGALGRMRSVELMPLALA
                     NARRLNKFGA"
BASE COUNT         1084 a         1483 c         1437 g         1247 t
ORIGIN      
        1 aggaagctgg tctaccgcaa gggtggcgtt ttcttcctgg aagatggtga aggtggtggc
       61 gtcttccaca tccaggcttc ctccgcggat gttccgggga ttgaggttga ccccgtcggt
      121 gtaccgcaga acgtggaagg gatcgacgaa gtttttggag tggaggagat cggagaaaca
      181 ccatcccaca tccggataat agacttccac ccaggcatga taccctcccc cgctgatctg
      241 gaccccccag tatttcttgg aaatccccca gtcatacccc gggggaagat acccatgggc
      301 gtaccgggcg gggatcccca agctgcggag catggcaatg gaaaggttgg tgtaccccac
      361 gcaggacccc cgtttactgt aaaagaccga ccggccgtct tgaggcgtgc cccggccggc
      421 gtcgtaggta aaattgtcca cgatccagtt aaagatgctc gtcaccgctt cctggaccgt
      481 ccggcttcct tgggcaagag cttccgcctg ggagaggatg ttcccttcat tcacagggat
      541 atctttctcc gggcccagaa aggacttcat ttggtaagga atctgctgtg tagaaatggg
      601 aaaaggtgcc gtggaattga ggggcgccag gttgacttcg atcagcaccc ttttgctgaa
      661 ttcatcttcc tgcaggactt tttggctgta agagctgttg gagtaattgg gaagaaattc
      721 cacgcccgtc gggttgacga tgtcgtagac aatggtgtgt ttcccgatga gaaccttggt
      781 ggcggagagg gccggagaca tccaccctgc tccgataatc ccgaccgccg tccacctccc
      841 gatccacagt aagagtcttt tccccaggcc tctccccgct tcaatcataa accaccatga
      901 cccggccgtt cttcagataa ccagtatagg gagcggtggt cagaacgacc tgtgcgtccc
      961 ggttggagat gaccacatcg gtttttttct ctccggtggc attcagaccg ttgaccacca
     1021 cggggtcggc agtcacccgc tgatttctgg ctgcggcatt cacgtctttg gcgtagccca
     1081 ccaaacccat ccgggcgata tcctgctgcc ccacgctctg gctgctgtac accagctctc
     1141 ccttttcatt cagaatcttg ggaaccagcg caggtcgcag gccaagcttc cgggcgtcta
     1201 tgatcagccc ggtgtaatcc agtttcttca ggtccaggtt ttcttccgcc ttggccggag
     1261 ctttggctgg cgtctggacc ttgggttcaa tcgagggagg aggggtacca ctcggctccg
     1321 ctaccggagg aggttggacc ttggtctcct ggggcgggga tggtgggggt ggaagcttgg
     1381 tatcctgcac cgttggtttg gggggcgcgg gaggaggcgg gggcggcgga acgggcttgg
     1441 gaaccggcgg aggcccgaga ctgggtatga ccggaggtgg aaggggctgc ggaagaggcg
     1501 ggctgggcat ggccggctga gacagagggg gcagttcctg gagcataacc gccaggagct
     1561 cccccgtcat gttgatcgtc accgtgactt caaccgcccc gtcgctcaag tatttggtat
     1621 ccacgaccct ggccccgatg accattcccc tgactcgggt caatacgcga tcactcttgg
     1681 tggtaaaact ctccaccgtg gtttccgaat cgacctgcac ccctttcacc gtctccagca
     1741 ggttgcgcag ggcatcggcc ttggctgcgc gctccgccat caggcgggcc tgggcctggt
     1801 tggtgattcc cttgggcggt gcaccgctcc ccttcgcggt gaccgctccc tggctccagt
     1861 tgatggcccc gctgggaagg ttctcgacaa cctccctccg gtcttgggcc atcgataggg
     1921 ggctggagag aataaggacc agaaaaacgg accagaacga aaaccccatt tttctcatgg
     1981 tttcctgttc ctttcttgct tttccaggcc tgactggcta ttaaaaacgt aacacaggtg
     2041 gggaaatact gtcaacgaat actggtttcc ttgcccgccc ccagtttttc atgcttgttg
     2101 gggttcaggg tgaaggaatt tggatcttca ggctcggcgg gcgcttatcc ttccaggaaa
     2161 ctcctccccc tgttccgcag ggagaggcct gagaggagat tcctccaagc agaaaagaga
     2221 accaaagcat gtttccgttt caatcccccc aggacaggcc cgattcaatg gaagaaccac
     2281 cgcctcagga tgatgatcca aatttctccg ccctgaaccc atgaggtccc ccctcctttc
     2341 cagagatctt attttcgaag gactcttttt taagaactgg gggtgggcaa gatactggtt
     2401 tctggttggg ggtcgctaaa tgccgatcgt tctttaagct ctgggcattt caagcacttt
     2461 agcgcatttt aggcactcta ggcattctag gcaatctacc gcattctctg caagaaggaa
     2521 tatctcactc atctttccac ccgtaatccc ccaccaaagg gacgaagaca catccggtca
     2581 catcttcttc gctgaattgg tccccttccc gggtcagctt gtaaagggtc tgggtgaacc
     2641 gaggacctgt ggggatgacc agccttcccc cttcggccaa ttgggatttc agggttgggg
     2701 gaacatcggg agacccggcg gtcaccatga ttccatcaaa tggggcctcc tctggccatc
     2761 ccctgcttcc gtcgcctgag aagaaagaga tgttggagta tcccaaatcc cccagaacct
     2821 tccgggccct ttcggtaagc gcgggaattc tttcaatagt aaagaccttt tgagccagct
     2881 ccgcaaggat ggcggtctgg taacccgacc cggtgccgat ttctaggacc ctttcctggc
     2941 ccttcagttc cagggattgg gtcataatgg ccaccatata aggttgagag atggtttgcc
     3001 cctccccaat ggggagagga cgatcctcat acgcggaatc ccggtaatct tcgaggacaa
     3061 aaagatggcg cgggattttg gccatggctt cgagcgtcct tcggtcggag atgcccctcc
     3121 gccgaagctg ctcatccacc atgatcagcc gttccctttg ataatcccgt ggcactccct
     3181 ttttgccttt cttccaaagc acggccatgg cagaaaccac actccgccga aggcgagact
     3241 aattcccgaa cgaatagcga agagacaaat cccaaattcc aaatcccaaa taagaagcaa
     3301 attcagacac ccgatgatca aaacacaaaa actgccccgc ttcgaccagg cttggatttt
     3361 tgaattttgg tttttgaaat ttatttggaa tttggtgctt ggatatctgt gcttgttttt
     3421 tctctaggtc ctctgttcct ccgtggtgaa atattctcac gccccgaatt tgttcaacct
     3481 tcgcgcgttg gccagggcca ggggcatgag ctcgaccgaa cgcattcttc ccagagcacc
     3541 ctggatacag ctgacttcgt caaatttcct gaccccgtcg actaggcagt attgagaatg
     3601 gagaatgacg gggagcggat gccagctgtg actggccagt ttggccggag tggcgtgatc
     3661 cccggtcacc accagaacat cgggattcag ggctctaacc ctgggaaggt atccatccac
     3721 ctcctcgatg actgacactt tagcgtcaaa attcccgtcc tctccccggg agtcggtctg
     3781 tttcacgtgg aggaaaaaga aatcataccg gggataattc tcctccagcg ccttgaactc
     3841 ctcctcgagg ctggctactt gagataggac ggtcatccct accaatcggc tgatcccgcg
     3901 gtacatggga taggcggcaa tggccagggc attcaggccg tagcgctctt tcatggaggg
     3961 ataccgggtg tgtttggcaa acccgcgtaa gagaaccatg ttggccttgg gttcatcagc
     4021 caggattctc ccaacctggg aaagaatgtc cctaactcgc cccgcagtct cttgcgcttc
     4081 gggaaccagg gctttgggct ccaacggggg agccccttcc cgctcggggt ccgtatcctg
     4141 aatatccccg cgaagcccct cccctctcag gaccaggatg ccccggtgtt ctttcaccgt
     4201 ctgcacgatc acttccagac ccgcgccggg tttcagcccc tggcggattt tctggcagac
     4261 gcgctggttg gtttccgtgg aaatgcgccc tgctctgcgg tcggtgactt ttcccccaga
     4321 gtccacggtg gcgaaattca tccgcgccac tacatctttt tcggtgaggg gaaagtcgat
     4381 ccccgtcgct tccagaattc cgcggccgat gttgtattgg accgggtcat atccgaagag
     4441 ggcgaaatgg gccggtccgc tgccaggagt aatccccggg gccacggggt ccagcaggcc
     4501 gcaggaggag gcttcggcga gtttatccag gttgggagtc cgggccacct gaagctcggt
     4561 cccccccttc ccttccatct gaattccccc cagcccgtcc atgatgagaa aaacgatctt
     4621 gctggagccg gagatcaaca actcagagac caagttcggg tccatatgat cctccccgga
     4681 taatgaaata cagaccttag atttcagaca tcagaccagt ctgaagtcta gggtctgaag
     4741 tctggttaag gccgattagc tctgcggggc gccaggcggc ggagtcgctg ccttcagcga
     4801 cgcttttctt ctcttttctc tttgcttggc ccttaaatgg aaccgatgtt tcatcctttt
     4861 gtgcttgctc ttcgaagtgg ccaaatagtt tcccccttca ttttattttc agatagaata
     4921 ttagaaaaat cgcttgaaat caaccatttt cctaatgcgg aattcggaat gcgaaataaa
     4981 aggaaaagaa aaaattccgc attcaactta ggtcgcttgt ggagcctgat aggttgaatt
     5041 cgcgctttaa gctttgcgtg atttggagtc ttcaaattcc ttttcccgaa ttctgcactc
     5101 cgaatttatg aatatgggtc ttctcacgct aacatcaaaa ggcttcgcct ggttttcgaa
     5161 gggccatcct tgggtcttcc gcgacgattt ggagaaaatc caggatgccc cggcaggcgg
     5221 gatcgtttcc ttggagaggc gaaacgggca g
//
LOCUS       DASPRR010000289        10614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_218420, whole genome shotgun sequence.
ACCESSION   DASPRR010000289 DASPRR010000000
VERSION     DASPRR010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218420"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1463
                     /locus_tag="VEL68_11055"
     CDS             252..1463
                     /locus_tag="VEL68_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ36546.1"
                     /translation="MKKKIVLLCIVALLAGSSSCGKKEEKAAEKVAAVTGLKVETVKA
                     FPQEDFYEAAATVRSKTTSVLSSRIMGYIVAIHVREGDRVRAGQLLVEIENREAAAQL
                     RKAQAGLREAQEMLQETERMIQANESAKTAAEAEQSLAASTFNRYQALLERKSVSQQE
                     FDEIQARYRGKTAEVERIKEMLRSMQAKKEQVLARIDQANAEISQAQVYFDYGRIQSP
                     LQGIVTQKQAEVGVLAAPGVPLLTLEDDARYRLEATVEESKLGKIRLGDSVRVIIDAL
                     GDQEMTGRVGEVQPIADPASRSSVVKIDLPESMIKKAPGRILRSGLYGKARFAVGKKE
                     IITIPHKAIFQRGQLLQVFVVDPSQTARLRLIKTGKIYGERIEVLSGLGEGEKIIVQG
                     LERVNEGNRIE"
     gene            1534..4758
                     /locus_tag="VEL68_11060"
     CDS             1534..4758
                     /locus_tag="VEL68_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXZ36547.1"
                     /translation="MTRQNLGLAGRLAHSFIDSKLTPLIVAASILLGIGAVLLLPREE
                     EPQIIVPMIDIFVQMPGASAKEVEERVTKPMEKLLWEIPGVEYIYSTSSPGQSLAIVR
                     FYVGEDEEKSIVRLNQKMFANFDLIPPGANPPLIKPRSIDDVSILALTLWSETQSPFM
                     LRRIAAQIHDQIKEVPEVSEVKIIGGQRRQVRVILDENRMSAYRLAPASILPVLAQAN
                     QQLQSGSFSSGNREFLVESGGFLTNADEVARVVVGVHQNRPVYLRDVARIEDGPEEPT
                     DYVLFGIGPSASLKNIQTPSPWVKSAVTISVAKRKGTNAIQIAEQVIHKVDHLKGLLI
                     PGDVQLTVTRNYGETAKEKSDELLLHMMIAIFSVAVLIWLAIGFREAGIVGIAIPVTL
                     ALTLTVFYLYGYTLNRITLFALIFSIGILVDDAIVVVENIVRHYHLPENRGRSILEVA
                     VEAVDEVGNPTILATFAVIAAIVPMAFVRGLMGPYMRPIPVGASAAMIFSLMVAFIVT
                     PWASLRLLRREKRGDHGEESWTTRLYRRAMTPLIQKPLYRYGFLMLVVLLLLGAMSLL
                     AVKFVHVKMLPFDNKSEFQVIVDMPEGTTLEETAAMTREIGEVIRKVPEVTDYQMYIG
                     TASPYNFNGLVRHYFLRRGPNVADIQVNLAPKGQRKAQSHDIAKRVRPLIDPIAARYQ
                     SRIKVAEVPPGPPVLQTLVAEVYGPDYSRQIEVARQVRGILEKTEGVVDVDWYVEDDQ
                     TRYRFVVDKEKAALDGVSAEQVAVTLRLAMSGMEAGLLHQAREKEDVPIVLRLPREER
                     SRVENLQAIRVMGKQGNLIPLGEIVRVEKDLGEKSIYHKNLMPVVYVTADVAGVEESP
                     VYAILKINQALDEMKLPEGYHLERYVARQPFTTEKLSMKWDGEWHITYEVFRDLGLAF
                     AAVLVLIYILVVGWFQSFKTPIAIMAAIPFSLVGILPAHGLLGAFFTATSMIGFIAGA
                     GIVVRNSIILVDFIELRLKQGMDLAEAVIDAGAVRFRPMMLTAAAVIVGASVILFDPI
                     FQGLAISLMAGEVASLFLSRMTVPILYYLSEQRRYRKKLEGFRE"
     gene            5433..6926
                     /locus_tag="VEL68_11065"
     CDS             5433..6926
                     /locus_tag="VEL68_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXZ36548.1"
                     /translation="MKKTLSIDLITRKRMDRFHLSSPFLYRSLWLLAVLVWGGMLALS
                     PAAHTEAAEKAGSPADFSMAIIQVAKQNIPAVVHIEVTERQEVVNPYLPFENDPFFRH
                     FFGTPKMPRKFQREVKGLGSGMIIDAQGHILTNHHVAGGATKLEVLLSNGARYPAKLV
                     GTDPKTDLAVIHIAAKDPLPYVTFGDSDRVEVGQWVVAIGHPRGLDQSVTQGIISAKH
                     RQGITDPTGYQDFLQTDAAINPGNSGGPLLNLKGEVIGVNAAIASESGGFEGIGFTIP
                     SNMALFVSRALIAHGKVVRGWLGVSLQELSPEVARTLHIGVTKGALVAEVVKGGPADK
                     AGIKKNDVVMEYRGKEIPEVSALRNEVASTPVGEEVKIVILRGGQKEEMLVKIGNLED
                     ATKFLAVAVKEHLGAEVRPLNSQEVEKYGLKANEGVAINWLEAKGPLQKAGFEIGDAI
                     LGIDDQPVQGMDSFVQMVSSLKPKQKISIVALDHRTGNQGVIELVVR"
     gene            6950..7114
                     /locus_tag="VEL68_11070"
     CDS             6950..7114
                     /locus_tag="VEL68_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36549.1"
                     /translation="MELEGLRGTYGHWAILVGTFLEGETILILGRFATSRGKVALPSV
                     ARDFYRKVQF"
     gene            7457..8956
                     /locus_tag="VEL68_11075"
     CDS             7457..8956
                     /locus_tag="VEL68_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ36550.1"
                     /translation="MKIGTKLTLYLSVIIVLVLCGFGYLDILSRRDILIRKMKAEVRS
                     AGRTLKVSLEEISPPEKREYIQGLIGAMGEYERNLGVVIYYARGNLVFHSPSLRGKIE
                     PYLDLTRRSMEKNAPQEEFETYGKVPVFVYTFPLEDREGKTIGGASILQETSSMEKEI
                     EQTKWSIFIMISVLILGTVTLVLLGTRKWINQPISNLMEGIQNFAGGNLDYQIDWKKA
                     DELSELARAFNQMAVDLRKAQERVIRETETKLELERGLRESEKLAIIGQLASGLAHEI
                     GTPLNIIYGRTELIQKGLEDKEGLQKNLDIVLRQTERITKIIQQLLGFVRKKKPEQKA
                     LSLPPLLECALELLDHQVRKQKVRVVKGRMDGLPSIIGDSDQLQQVFLNLILNAIHSM
                     PEGGTLSISGSSKRISKDGRPGDQRSYLEVSVEDTGVGMEKEVLQNIFTPFFTTKDTG
                     TGLGLMVTQGIVQDHEGWMEVESEVGRGSRFKVYLPSLGEEVIGEPVRQ"
     gene            8937..10286
                     /locus_tag="VEL68_11080"
     CDS             8937..10286
                     /locus_tag="VEL68_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ36551.1"
                     /translation="MNPSGNKILVVDDDREMASLLSDVLRGEGFSVFPQSDSLEASKI
                     LQREEFDVLITDLKMKGLKGLDLLEEAKKVAPLTPVIIITAFGTIESAIQAMKLGAYD
                     YITKPFQMDQIVLIVKKALENRWLKKEVVRLKKEVESRFHFHQLIGKSPSMQKLFDLV
                     ERIKESSSNVLITGESGTGKELVAKAIHYSGARKEGPFIAVNCAAIPETLLESELFGY
                     KKGAFTDAKADKKGLISEAREGTLFLDEITEMSPLLQAKMLRVVEERQVRPLGDTNSH
                     PVDVRFISASNRDMKDLIRQGGFREDLYYRLKVIDIELPPLRERKDDIPLLVQHFIHR
                     FGQEPKKVSGVSEETLRILIDYSWPGNVRELENVIQRAITLCQQETLFPEDLPASMIQ
                     ELDEDLVEKGLRKKYSLDQLEKEYIQKVLIEAGGNKTRAAEILGLDRKTLYRKLEEI"
BASE COUNT         2563 a         2676 c         3059 g         2316 t
ORIGIN      
        1 agacggctta ccttaggact cgattgaatc tgatttcctc aaggtatgat tattatattg
       61 gatatgcccg agtgctgatg gccaccggaa agctgaccga cgtgaccccc ttcaattcat
      121 aaattcccaa cgggaggcag atgaacgcgg gttttcagga tatagaataa atgaaaggac
      181 ttggtttcga ctcttattct ttctctgcgc tggtctgctt taatctgcct cccgatcatt
      241 tgaggatgca aatgaaaaag aaaattgtcc tcctctgcat tgttgcgctt ctggccggct
      301 cttcttcctg cggcaagaag gaagagaaag cggcggagaa ggtagccgcc gtgaccgggt
      361 tgaaagtgga aaccgtgaag gctttccccc aggaagattt ttatgaagct gcggcaaccg
      421 ttcggtccaa aaccacatcc gtcctctcct cccggatcat gggatatatt gtggccattc
      481 atgtgcggga gggagaccgg gtccgcgccg ggcagctact ggtggagatc gaaaaccggg
      541 aagctgccgc ccagttgcga aaggcccagg ccggactccg agaggcccag gagatgctcc
      601 aggagacgga gcggatgatt caggccaacg aatcggccaa gaccgcagca gaagcggagc
      661 aatcccttgc ggcttcgacc ttcaaccgct accaggcgct gctggagcga aaatccgtaa
      721 gtcagcagga gtttgacgaa attcaagccc gttaccgagg gaaaacagca gaggtcgaga
      781 ggatcaagga aatgctgcga tccatgcagg ccaagaagga gcaggtgctg gcccgaatcg
      841 atcaggccaa tgcggagata tcccaggccc aggtctactt cgattacggc cggattcagt
      901 ctcccctcca agggatcgta acccagaagc aggccgaagt gggagtgctg gccgccccgg
      961 gagtgcccct tctgacccta gaagacgatg cccgctatcg cctggaagca acggtggaag
     1021 aatccaagct cggaaagatt cgtttgggag attcggtccg ggtcattatt gatgccctgg
     1081 gggatcagga aatgacaggc cgggtggggg aggtccagcc catcgcggac ccggccagca
     1141 ggagctccgt ggtgaagatc gacctcccgg agagcatgat caaaaaggcg cccgggagaa
     1201 ttctccggtc cggactgtac ggcaaagccc ggtttgcagt gggaaagaag gaaatcatca
     1261 ccatcccgca taaggcaatc ttccagagag gtcaactgct gcaggtattt gtggtcgatc
     1321 cttcccagac ggcccggctg aggctgatca agacgggaaa gatttatggg gagcggattg
     1381 aagtcctttc cgggctgggg gagggggaaa agatcatcgt gcagggcttg gaacgggtga
     1441 atgaggggaa tcggatcgag taggggcgac ccggcgggtc gcccctgcga actacgcaat
     1501 gctttttgag acccaaagag gaaactacgt tcaatgacaa ggcaaaacct gggcttagcc
     1561 ggtagattgg cccattcctt catcgactcc aagctgactc ctctcatcgt ggccgcttcg
     1621 attcttttgg gcatcggggc cgttctgctg ctcccccggg aagaagaacc ccaaatcatc
     1681 gtgcccatga tcgacatctt cgtccagatg cccggcgctt cggccaaaga agtcgaggaa
     1741 cgggtgacca aacccatgga gaaactcctc tgggaaatac ccggggtgga gtacatctat
     1801 tccacctcat cgcccgggca atccctggcc atcgtccgtt tctacgtggg cgaggacgaa
     1861 gagaagtcca tcgtccgcct caaccagaaa atgttcgcca acttcgacct cattcccccc
     1921 ggggccaacc ctcccctgat caaaccccgc tccatcgatg atgtttccat cctggccctg
     1981 actctctgga gcgagaccca gagccccttc atgctccggc ggattgccgc ccagattcac
     2041 gatcaaatca aggaggtgcc ggaggtctcc gaagtgaaga tcatcggcgg ccagcggagg
     2101 caggtccggg tgatccttga cgagaatcga atgtcagcct atcgtctcgc cccggcgtcg
     2161 atccttccgg tcctcgctca ggccaaccaa cagctccagt cggggagctt ctcttccggg
     2221 aaccgggaat ttctggtgga aagcgggggc ttcctgacca acgccgacga agtggcccgg
     2281 gtagtcgtag gggtccacca gaaccggccc gtttacctgc gggatgtggc gcgcattgaa
     2341 gacggccccg aagagccaac ggattacgtc ctcttcggga tcggaccgtc cgcttctctg
     2401 aagaatatcc agaccccttc tccctgggtc aagagcgcgg ttacgatttc agtggccaag
     2461 aggaaaggga ccaatgccat ccagatcgcc gagcaggtca ttcacaaggt ggatcatttg
     2521 aaaggcctac tgatcccggg ggatgttcag ctcacggtaa cgcgcaatta cggggaaacc
     2581 gctaaagaga agtccgacga gctcctcctc catatgatga tcgccatctt ttcggtggcg
     2641 gttctgatct ggctggccat cgggtttcgg gaagcaggca tcgtgggtat tgccattcct
     2701 gtgaccctgg ccctgaccct caccgtcttc tatctttacg ggtatacctt gaaccggatc
     2761 accctttttg ccctcatctt ctccatcgga attctggtgg atgacgccat cgtggtggtg
     2821 gagaacatcg ttcggcatta ccacctcccc gagaaccggg gaaggtccat tctggaagtg
     2881 gccgtggagg ccgtggatga ggtgggaaat cccaccatcc tggccacctt tgcggtcatc
     2941 gcggccattg tacccatggc cttcgtgcgg ggtctcatgg gaccctacat gcggcccatt
     3001 ccggtgggtg cctcggcggc catgatcttc tccctcatgg tggccttcat cgtaactccc
     3061 tgggcaagcc tgcggcttct ccgccgggaa aagaggggag atcatggaga ggagagctgg
     3121 acaacccggc tctaccggag agccatgact cctcttatcc aaaaacccct gtaccgctat
     3181 ggtttcctca tgctggttgt cctgcttctt ctgggggcca tgtccctatt ggcggtcaag
     3241 tttgtgcacg tgaagatgct acccttcgat aacaagagcg agtttcaagt tattgtggat
     3301 atgcccgagg gaacgaccct ggaagagacc gcagctatga cccgggagat cggggaagtc
     3361 atccgcaagg ttcccgaagt aaccgactac cagatgtata tcggcacggc ttcgccatac
     3421 aacttcaacg gactggtgcg ccactatttt ctccgtcggg gcccgaacgt ggccgacatt
     3481 caggtcaacc tggctcccaa ggggcagaga aaggcccaga gtcacgacat cgccaagcgc
     3541 gtccgtcccc tgatcgaccc catagccgcc agatatcaat cgagaatcaa ggtggccgaa
     3601 gttccgccgg gtccgccggt tctgcagacc ctggtggccg aggtgtatgg tccggattac
     3661 tcccgtcaga ttgaggtggc acgccaggtc cgcggcatcc tggagaagac cgagggggtt
     3721 gtggacgtgg actggtacgt ggaggacgac cagacccgat accgattcgt ggtggacaag
     3781 gagaaagccg cgctggacgg ggtctcggcc gagcaggtgg ccgtgaccct acgtctggcc
     3841 atgtcgggga tggaggcagg cctcctccac caagcgaggg agaaggaaga tgttcccatt
     3901 gtgctccggt tgccccggga ggagcggtca agggtggaaa acctccaagc tatccgggtg
     3961 atgggaaagc aggggaacct catccctctg ggggaaatcg tccgggtgga gaaggacctg
     4021 ggggagaaat ccatctatca caagaacctc atgccggtgg tatatgtgac cgccgacgtg
     4081 gccggagttg aggagagccc ggtttacgcc attctaaaga tcaaccaggc cctggatgag
     4141 atgaaactgc ccgaaggata tcacttggag aggtacgtgg cccggcagcc tttcaccacg
     4201 gaaaagctgt ccatgaagtg ggatggagaa tggcacatca cttatgaggt cttccgcgac
     4261 ctcggcctgg cctttgccgc ggtcctggtg ttaatttaca ttctggtggt agggtggttc
     4321 cagtccttca aaacccccat cgccatcatg gccgccattc ccttctccct ggtgggaatc
     4381 ctgccggccc atggcctgct gggagccttc ttcaccgcca cttccatgat cggtttcatc
     4441 gctggcgcag ggatcgtggt gcgcaactcc atcatcctgg tggactttat cgaactgcgc
     4501 ctgaagcagg gcatggacct ggccgaggcg gtcatcgacg ccggggccgt gcggtttcgt
     4561 cccatgatgc tcaccgcagc cgcagtgatc gtaggggctt cagtgatcct gttcgacccg
     4621 atctttcagg gcctggccat ttcgctcatg gccggagaag tagcttcatt gttcttgtcg
     4681 cggatgaccg tgcccatcct gtattacctg agtgagcaac ggagatacag gaagaagttg
     4741 gaagggttca gagagtaatc ctgcgtgctg agaaaaaaca tccaattttt tggaggcata
     4801 gacgaacgca ggtttattat aatcttgacc gcaaaaggaa ttcttatggg cgcaaggatg
     4861 aacaaaacgg atagaagtgg ccggtactta tcgccttggc cagaaatgtg ctatgaagga
     4921 aagcccaagc tctggttttg aacggctgag ttcccctctt ccatggtttt tacggggcag
     4981 ctgacattcc tcctggaaaa aatctaccac cgcctattga gcagcaagac cgcttttgcc
     5041 attcaacggc atctccattg gagcgcgatt accacggtaa tattgccgat tcgcgtaaaa
     5101 ctgtaaaaag tccattaaaa tacagtcttt taccccccta aatacagtcc ctaccctatt
     5161 ttagccctct tttctttccc cttagaattt taaagagggt ttaaaaaagg gattctctaa
     5221 ggaatgaaca tgcaaaatgc ttgattgagg ccttctccgc ggtcgagtgt caaaattgga
     5281 attctaaagc cccatccgaa actacctgcc ttgttctgag ctgcgggcga ccatagtcgg
     5341 aaaataggcc ttaatctttt tcctgtccgg aaggcctcaa gaattcagga gcattcaaat
     5401 caagcataat taactatcag gagggaattt ccatgaaaaa gactttgtca atcgatctga
     5461 ttaccagaaa aaggatggac cgtttccacc tatcctcgcc ctttctttat cgttcgctat
     5521 ggcttttggc tgttttagta tgggggggaa tgttagccct atctccggca gctcataccg
     5581 aggcagcgga gaaggcgggg tcgcccgctg atttcagcat ggccattatc caagtggcca
     5641 aacagaacat ccctgcagtg gtgcatatag aagtgaccga acggcaagaa gtggtcaatc
     5701 cttatttgcc tttcgagaac gatccattct tccgccactt ttttggcaca cccaagatgc
     5761 cccgaaaatt ccagagggaa gtaaaggggc tggggtcggg aatgatcata gatgcccagg
     5821 gtcatatcct aaccaaccac catgtggcgg gaggcgcaac caaactcgag gtgctgcttt
     5881 ctaatggcgc gcgatacccg gccaagttgg tggggaccga tcccaagaca gacctggccg
     5941 tgatccatat cgccgccaaa gacccccttc cctatgtgac tttcggcgac tcagacaggg
     6001 tggaagtggg gcaatgggtg gtcgcgatcg gacatccccg gggtttggat caaagtgtca
     6061 cccaggggat catcagcgcc aaacatcgcc agggcattac cgaccccacc ggctaccagg
     6121 attttctcca gaccgatgcg gccatcaacc cgggcaacag cgggggacct cttctaaacc
     6181 ttaaggggga agtgatcggg gtcaacgcgg cgatcgcttc ggagtcgggg ggctttgaag
     6241 gcatcggctt taccattccc agtaatatgg ccctcttcgt ctcccgagcc ctgattgccc
     6301 atggcaaagt agtgcgggga tggttggggg ttagcctgca ggagctgtct ccagaggtag
     6361 cccggacttt gcacatcgga gttacgaaag gagctctggt ggccgaggtg gtcaaaggag
     6421 ggcctgcgga taaggctggc atcaaaaaaa atgatgtggt gatggagtac cgggggaaag
     6481 agattccaga ggtaagtgct ttgcggaatg aagtagcatc cacgcccgtg ggtgaggagg
     6541 taaagatcgt tatcctgcgt ggcgggcaaa aagaggaaat gttggtcaag ataggaaatt
     6601 tagaggacgc gaccaagttt ctggccgtgg ccgtgaagga gcatctgggt gcggaagtaa
     6661 ggcccttgaa ctcccaggaa gttgaaaagt atggtttgaa ggccaacgaa ggggtggcga
     6721 tcaactggct ggaggccaaa ggtcccctcc agaaagccgg ttttgagatc ggggatgcga
     6781 ttttgggaat cgacgatcaa cctgttcagg ggatggacag ttttgtccaa atggtaagct
     6841 ccttaaaacc caaacaaaaa atatccatag tggccctcga ccaccggacg ggcaatcagg
     6901 gagtgatcga gctagtggta cgataaagta agaagcaagc ccttcaggaa tggagctgga
     6961 aggtctgagg ggcacttatg gacattgggc gattttggtg ggaaccttcc tggaaggaga
     7021 aaccatcttg atcctgggac gctttgccac ctcccgggga aaagtggctt tgccctccgt
     7081 ggcgagggac ttctaccgga aggtacaatt ttgaagggga gaatgggagg cttgccggcg
     7141 caacgcggga cagattgaaa gcatctaaaa ggtgagatta tttatgttga atatggaatc
     7201 cgataaattg ggagatgctc tgagcggatg gcggctaatt tttcgggcct tgggccacag
     7261 gaattaccgc tttttttcgg ttcgcctgcc tttggcggtg tggattaagg atggcattga
     7321 cagtgtggct gaaatggtgg ctgggccttg caggattccg gacgggtcaa gctggcgggg
     7381 aagaagacct ttatgaaggg agccccccgg cgtttcttcg gggggttttg ggaattatgg
     7441 aaggaagatt tgggcagtga aaatcggtac caagctcacc ctttatcttt cggtcatcat
     7501 cgtccttgtc ctttgtggat tcgggtacct cgatattctt tcccgtcggg acatcctgat
     7561 cagaaagatg aaagccgaag tgaggagcgc cggccggacc ctcaaggtct cgctggagga
     7621 aatatcgcca ccggagaaaa gggagtatat tcaaggctta atcggtgcta tgggggagta
     7681 tgaaagaaat cttggtgtcg tgatctatta cgcaagggga aatctcgtct ttcattctcc
     7741 ttcgttgaga gggaaaatcg aaccctattt ggacctgacc agaaggtcca tggagaaaaa
     7801 tgcccctcag gaggaattcg agacctatgg aaaggttccc gtattcgtct acacctttcc
     7861 tttggaagat cgagaaggaa agactatcgg gggtgcttcg attcttcagg aaacttcttc
     7921 catggaaaag gagattgagc aaaccaaatg gagcattttt atcatgatct ccgtgctcat
     7981 tctgggtacg gtcaccctgg tccttttggg cacaaggaaa tggattaatc agcccatctc
     8041 caatctgatg gaaggaattc agaattttgc cggggggaat cttgattacc agatcgattg
     8101 gaagaaagcg gacgagctct cggagctggc ccgggcgttt aatcagatgg cggtggattt
     8161 gagaaaagcc caagaaaggg tcatccgaga aacggagacc aaattagaac tggaaagagg
     8221 acttcgggaa tcggaaaaac tggccatcat cggccagttg gcttcaggcc tggcccacga
     8281 gatcggaacc cccctcaaca tcatttatgg aaggacggaa ttgattcaaa agggcctgga
     8341 agataaagaa gggctgcaaa agaatttgga tatcgtcctc cgccagaccg agaggattac
     8401 caagatcatc caacagctcc tggggtttgt ccggaagaaa aaaccagagc agaaggccct
     8461 gagcctaccc ccccttttgg agtgtgccct tgaacttctc gatcaccagg tccggaagca
     8521 gaaggttagg gtggtaaagg ggcggatgga cggccttccc tcgatcatcg gggattccga
     8581 ccagctccag caggttttct taaacctcat cctgaatgcg attcattcca tgcccgaggg
     8641 aggaaccctg tctatttctg gttcgtccaa aaggatttcc aaggatgggc gcccggggga
     8701 ccaaaggtcc tacctggagg tctccgtgga ggatactggg gttgggatgg aaaaggaagt
     8761 cctgcagaac attttcaccc ccttctttac caccaaggat accggaacgg ggttaggttt
     8821 gatggtcacc cagggaatcg tccaagatca tgaaggctgg atggaggtgg aaagtgaggt
     8881 cggcagaggg tccaggttta aagtctatct cccttccttg ggggaggagg tgataggtga
     8941 acccgtccgg caataagatc ctggtggtcg atgatgaccg agagatggcg agcttgctat
     9001 ccgacgttct acgaggggag gggttctccg tctttcccca gagcgatagt ttggaagcct
     9061 cgaagattct ccagagggaa gagttcgacg tcctgatcac cgatttgaag atgaaaggat
     9121 taaaaggtct ggacttgcta gaggaggcca agaaggtagc ccctctgact cccgtgatca
     9181 ttatcaccgc ctttggaacc atcgaatcgg ccatccaggc catgaagttg ggcgcctacg
     9241 actatatcac caagcccttc cagatggacc agatcgttct aatcgtcaag aaagccctgg
     9301 agaatcgctg gctgaagaag gaagtcgttc gcttaaaaaa ggaggtagag tcgagatttc
     9361 acttccacca actgatcggc aaaagccctt ccatgcaaaa acttttcgac ctcgttgaaa
     9421 ggatcaagga gagctcaagc aatgtcttga ttaccgggga gagtggaacg gggaaggagt
     9481 tggtggccaa agcaatccat tattccggcg cgaggaaaga gggccccttc atcgcggtca
     9541 actgtgcggc cattccggaa acgctcctgg aaagcgagtt gttcggctat aagaaggggg
     9601 ccttcaccga tgccaaagct gataagaaag ggttgatttc ggaagccagg gagggcacgc
     9661 ttttcctgga cgagatcacc gaaatgtccc ctttgctcca ggcgaaaatg ttgcgagtcg
     9721 tcgaagagag gcaggtccgt ccgctagggg atacaaattc ccatcccgtc gatgtgcggt
     9781 tcatctcggc atcaaaccgg gatatgaagg atttgatccg ccagggggga ttccgggaag
     9841 acctgtacta tcggctgaag gtgatcgata tagagttacc gccgctccgg gagcgaaaag
     9901 acgatatccc tcttctggtt cagcatttta ttcatagatt cgggcaagag ccgaagaagg
     9961 tctccggagt ttccgaggag acgcttagaa tcttgatcga ttattcatgg cccgggaatg
    10021 tgagggaact ggaaaacgtc atccagaggg ccattaccct atgccagcag gaaaccctct
    10081 ttccggagga tttacccgcc tctatgattc aggagctgga tgaagacctg gtcgagaaag
    10141 gccttcgcaa gaaatattct ctggaccagt tagagaagga gtacatccag aaagtactca
    10201 tcgaagcagg cgggaataag accagggccg ctgaaatcct gggcttggat cgaaaaaccc
    10261 tctaccgaaa gctggaagaa atttaaagca agatactgga tataggatgc ttgatactgg
    10321 attccagcaa aagatattag atactaattc tgaatagagg atctccgata tcctccatcc
    10381 agcattcagt atccagtatc aaacatccag ggcagagcat ccattcccat cgccccattc
    10441 tgccacacgt tgtttcttta ctccccaatc ttcctttctt cttatccgcc tccgattttg
    10501 agaagctatg aaattataag gagttttgcc tttgtccccg ttggcaaccc ttttgctttg
    10561 gtaaatggaa aagagggggg aaggactgaa ggagaaccaa aaaaagtaga tagt
//
LOCUS       DASPRR010000290         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1356676, whole genome shotgun sequence.
ACCESSION   DASPRR010000290 DASPRR010000000
VERSION     DASPRR010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356676"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VEL68_11085"
     CDS             complement(<1..465)
                     /locus_tag="VEL68_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ36552.1"
                     /translation="MRLLVIEDEKKVARFVKKGLEEEGYAVDLAFDGEEGLAMVLDQV
                     HDLIILDIALPKIDGLQVLKKLRERAVRTPVLLLTVRATIEDKVLGLDSGADDYLTKP
                     FVFQELLARIRALLRRKAEAGPPLLRVEDLALDPARHLVTRGGERIDLTSKEF"
     gene            complement(633..>2743)
                     /gene="mgtA"
                     /locus_tag="VEL68_11090"
     CDS             complement(633..>2743)
                     /gene="mgtA"
                     /locus_tag="VEL68_11090"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HXZ36553.1"
                     /translation="TIPGDCAILEARDLFVNEAALTGETFPVEKTQGKHPAETPMTLR
                     SNALFLGTHVVSGQARVVVVRTGRATEFGKISERLKLRPPETEFERGVRRFGYFLMEV
                     TLLLVISIFAVNVFLKRPVLDSFLFTLALAVGLTPQLLPAIISINLSHGARRMALQKV
                     IVKKLSAIENFGSMNVLCSDKTGTLTEGTIQVHSTLDAQGQKSDRVLHYAYLNAFYET
                     GFLNPIDEAIRNHRPSDVSSYRKLDEVPYDFIRKRLSILVAKGDTHWMVTKGALPNVL
                     SVCSRVETAQDPAEDIHLHEEKIRNGFESLSRQGFRVLGISYRNMGSEHSIQKDQEKD
                     MTFLGFIVLFDPPKEGVSETIQKLRELGVSLKIVTGDNRWVAMAVSQQVGGRTPQLLT
                     GGDLGKMSEDALARRVNEVDVFAEVEPNQKERVLRALQKAGNVLGYMGDGINDVPALH
                     AADVSISVDRAVDVAKGTADIVLLEKDLGVLLEGIREGRRTFANTLKYVFMATSANFG
                     NMFSMAGASLFLPFLPLLPKQILLTNLLTDFPEMTIATDHVDPEMVEQPRRWNLAFIR
                     KFMVTFGVLSSVFDYLTFAVLLLILRATPEQFRTGWFLESVISASLVVLVIRTRRSLF
                     KSKPGKALTWATLLIIGATSILPFTPLAVLFDFHLLPSFFFLWIGGILFLYVFSAESV
                     KNHFYRGMVSGGFKNPQGEG"
BASE COUNT          655 a          750 c          721 g          617 t
ORIGIN      
        1 gaattccttg ctggtcaggt caattctctc tcctcctcga gtgaccaggt ggcgggccgg
       61 gtctaaggcc aggtcttcaa cccgaagaag agggggtccg gcttccgcct tccgtcgaag
      121 caaggcccga attctggcca gaagttcctg aaagacgaaa ggtttggtga gataatcatc
      181 cgctccggaa tccaggccca ggaccttatc ctcaatggtc gctcttaccg tgaggaggag
      241 caccggggtg cggacggccc tttcccgtag ctttttgagg acctgaagtc catcgatctt
      301 agggagggcg atgtcaagga tgattagatc gtgaacctgg tcgaggacca tggcgagccc
      361 ttcttctcca tcgaaggcca ggtccacggc atagccctct tcttccaggc ctttctttac
      421 gaatctggca accttttttt catcctcaat gaccaaaagt ctcatgaagg gatttctatt
      481 ggcaattctt caaattttgt ttccaaccca gggttgcatt tccgatgctt gacagtcgaa
      541 atgaaagtga acctcaataa aattataact cctattaaga tttattcaaa ttcacctgtt
      601 attttacgct ttcgactttg ccgccgaatc ggtcagccct ccccctgagg gttcttgaac
      661 ccacccgaaa ccattccccg ataaaaatga ttcttgacgc tctccgctga aaagacatag
      721 agaaaaagga ttcctccgat ccacagaaaa aagaaggaag gcaagagatg aaaatcaaaa
      781 aggaccgcca aaggagtaaa aggaaggatc gaagttgccc caataatcag aagggtcgcc
      841 caggttaaag ccttccccgg tttgcttttg aataaggacc tgcgggtacg gatgaccaac
      901 accacgagtg aggcagagat caccgattcc agaaaccacc cggtgcggaa ctgttccggg
      961 gtggcccgga ggatgagcag gaggacggca aaggtcaggt aatcgaagac cgaactcaag
     1021 accccgaagg tcaccatgaa tttccggatg aaggccaggt tccagcggcg gggctgttcc
     1081 accatttcgg ggtccacgtg atccgtggcg atggtcattt ccggaaaatc ggtcagcaga
     1141 ttggtgagca gaatctgttt cggaagcagg ggcaggaagg gcaggaacag ggaggctccg
     1201 gccatgctga acatattgcc gaagttggcg ctggtggcca tgaagacata cttcagggtg
     1261 ttagcgaagg tccttctccc ttcccggatg ccctccaaca gaacccccaa atccttctcc
     1321 aagaggacaa tatccgcggt gcccttagcc acgtcaacgg cccggtccac ggaaatgctc
     1381 acatcggcgg catgaagggc cgggacatca ttgatgccgt cgcccatgta ccccagaacg
     1441 tttccggctt tctgcagcgc ccgaaggacc cgttccttct ggttgggctc gacctcggcg
     1501 aatacatcta cttcattcac tctccgggcc agggcatctt cgctcatttt ccccaggtcc
     1561 cccccggtca aaagctgcgg ggtccgtcct ccgacctgct ggctgacagc catggctacc
     1621 caccgattat cgccagtcac gattttcagg gagacaccga gctctcgtag cttttggatg
     1681 gtctcagaaa ccccctcctt gggagggtcg aagagaacga taaagcccaa aaaggtcatg
     1741 tccttttctt ggtccttctg gatcgaatgt tccgaaccca tattccgata ggagattccc
     1801 agaacccgga atccctgccg gctgagggat tcaaaaccgt tccgaatttt ctcttcatgc
     1861 agatggatat cctccgcggg atcttgcgcc gtttccaccc gggagcaaac ggacagtaca
     1921 tttggcaacg ctcccttggt caccatccaa tgggtgtccc ctttggcgac caagatgctc
     1981 agccttttgc gaatgaaatc atagggaact tcgtccagtt ttcggtaaga ggagacatcg
     2041 gaagggcggt ggttgcgaat ggcttcgtca atgggattca gaaacccggt ctcataaaaa
     2101 gcattcaggt aggcatagtg aagaactcta tcactcttct gtccttgggc atccagggtt
     2161 gagtggactt ggatggttcc ttcggtcagc gtaccggtct tatcggagca gaggacgttc
     2221 atgctgccaa aattctcaat ggcggataac tttttgacga tgaccttttg aagggccatg
     2281 cgcctagccc cgtgggatag gttaatactg atgatcgctg gcaacagctg cggagtcaat
     2341 ccgactgcca gggcgagagt aaataggaag gaatccaaga ccgggcgttt caaaaagaca
     2401 ttcaccgcaa aaatagaaat gaccaacagg agagtgacct ccatcagaaa gtacccgaac
     2461 cttcttaccc cccgctcaaa ttcggtctcc ggggggcgga gtttcagtcg ttcggaaatt
     2521 ttcccgaatt cagtggcccg cccggtccgg accaccacca ccctggcctg cccgctcacc
     2581 acgtgggtgc ccaggaataa ggcgttggac cgcaaagtca tgggcgtttc ggccggatgc
     2641 tttccttggg ttttctctac gggaaaagtc tcccccgtca gagcggcttc attcacgaaa
     2701 aggtctctgg cttccaggat cgcgcagtcc ccgggaatgg tat
//
LOCUS       DASPRR010000291         6184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1514388, whole genome shotgun sequence.
ACCESSION   DASPRR010000291 DASPRR010000000
VERSION     DASPRR010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514388"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="nikC"
                     /locus_tag="VEL68_11095"
     CDS             <1..835
                     /gene="nikC"
                     /locus_tag="VEL68_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HXZ36554.1"
                     /translation="QVREVWRRLKRNRAAIVGGIIVILFVATAILAPWLSPYHPNEGD
                     LTKRLKAPDREHLLGTDPLGRDVLSRVIYGARISLQIQIVAVVIAMFVGTILGMVGGY
                     YGGTFDNLIMRLMDILLAFPGIFLAIAIIAVLGPGLTNLMLAAGIYSIPQFARIVRGS
                     VLSLKEKEFVEAARAVGEKDFNILFRYLLPNSMAPIIVQTTLRMATVLLTASGLSFLG
                     LGVQPPTPEWGAMLSNARAYLITAPHVATVPGLAIMMVVMGFNLFGDGLRDSLDPRLR
                     D"
     gene            1345..2328
                     /locus_tag="VEL68_11100"
     CDS             1345..2328
                     /locus_tag="VEL68_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36555.1"
                     /translation="MATLLSVKDLRVHFFTAGRTVRALDGVSFEIEEGKTFGLVGETG
                     CGKSVTALSILRLIPFPPGKIMGGEIHFRGNNLLSLSEEEIRSIRGKEISMIFQEPMT
                     SLNPVFRIGDQMSEVIRLHQGLDRSKAWAKAEEMLERVRIPDTRRVAKQFPHQLSGGM
                     RQRVMIAMELSCHPYLLIADEPSTALDVTIQAQILRLIREMKDELHTSILLITHDLGV
                     IAEMCDRVGVMYAGSIVEEAGVEEIFESPRHPYTQGLWGAIPRIDDEKENLSVIPGMV
                     PDLSNPPAGCRFHPRCAHRSDVCEQSIPPLFAVAPGHLVACFLHSERRGHG"
     gene            2321..3286
                     /locus_tag="VEL68_11105"
     CDS             2321..3286
                     /locus_tag="VEL68_11105"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HXZ36556.1"
                     /translation="MAEPLLQVRGLKKHFPLARGWRKEKALVRAVDGVDFSIRRGETL
                     GLVGESGCGKTTVGRLILRLIDPTEGEVLFEGKNLASLEEKAIHPLRRFFQIIFQDPF
                     ASLNPRMTVSEIVGRPMEVHGLAERSQREEKVANLLKSVGLMREHLGRYPHEFSGGQR
                     QRIGIARALATQPSLIVADEPTSALDVSVQAQILNLLKKLQRDLGLTYLFISHNIGVV
                     RHISDRVAVMYLGKIVEMAEKKILFQSPAHPYTKALLAAVPSLDPKRKREEVILEGDV
                     PSPINPPCACRFHTRCSYALNKCRQEEPLFHQIEEGRWISCHLYP"
     gene            3415..4575
                     /gene="iadA"
                     /locus_tag="VEL68_11110"
     CDS             3415..4575
                     /gene="iadA"
                     /locus_tag="VEL68_11110"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HXZ36557.1"
                     /translation="MFTLIKGGTLYGPKKMGVKDILVVGKTIAKISERIDLSDDFNVQ
                     IVWATGKIITPGFIDLHVHILGGGGEGGPRTRTPEITLSQITRAGVTTVVGCLGTDDV
                     TRKIETLLAKAMQLEEEGISTFIYCGSYQFPPPTITGCVRGDIALIPKVIGVGEIAIS
                     DHRSSQPTFEELTRIAAEARVGGMIGGKPGLVHLHMGSGPRRLDPIIRIVKETEIPIG
                     QFLPTHLTRTPELLEQAIQFARLGGNIDFTVKGKDLSFPLTTQQALQKALDAGVSMDQ
                     ITLSSDSNGSIPIFDERGNLIRLAVGDIQNLFREWRGLVREGFPIEEILKLVTANPAK
                     RIGAFDSKGSLAEGKDADLLILNPDLEIDSVMAKGRLLILQNQFLVKGTFEN"
     gene            4642..5946
                     /locus_tag="VEL68_11115"
     CDS             4642..5946
                     /locus_tag="VEL68_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36558.1"
                     /translation="MSKKNQTLLILTVSMALGYMPWYNFSAVLKYLADEFHLTTSDTG
                     WTLAAFQAGYVIVVGGTGWLGDRVSLKKIVLWATLITGVFSALFVWGAGGKESIIALR
                     LITGLAAGAIYVPGLALLSQWFSPRERGGALGAYTGALVASYAGGYLVAGRLAATYGW
                     RTGVLWTSLPAILAALLIYLLVRDAPASVQSSGSGKRQGMAERAQDLSGREGGESEIL
                     EAPPGGFKGPALLSVSYMGHMWELYAFWGWIGPFLVSSALAVGMPPDGAVKWGATFAA
                     AIILIGAPATWGWGIVADRKGRTWAIMVAGVLSVGAEFFVGYLYGHSLSILVLAGAWI
                     GFWVIADSAIYKAGLTEMVLPRIRSTSLGIQSVVGFSVTIISPMVFGKILEYYNGPVA
                     AAQAKVWGPAFVALGIGGMVAPAAAWMLRRQRQAKLMAGGKK"
BASE COUNT         1359 a         1644 c         1807 g         1374 t
ORIGIN      
        1 tcaggttcgg gaggtctgga ggagactcaa acggaaccgg gccgccatcg tgggggggat
       61 catcgtcatt ctttttgtgg ccaccgccat cctggccccc tggctctctc cgtaccatcc
      121 gaatgaaggc gacctgacta aacgcttgaa ggctccggat cgcgagcacc tcctgggaac
      181 ggacccgctg gggagagacg tcctcagccg ggtcatttac ggggcccgga tttccctaca
      241 aatccagatc gtggccgtgg tgatcgccat gttcgtcgga accatcctgg gcatggtggg
      301 gggatactac gggggaacat tcgacaacct catcatgcgg ctcatggaca tactcctggc
      361 gttcccggga atattcctgg ccatcgccat tatcgccgta ttgggcccgg ggctgaccaa
      421 tctcatgctg gccgcgggta tctactccat tccccagttc gcccgtatcg tccgcgggtc
      481 tgtcctttct ctgaaagaaa aggagttcgt cgaggcagcc agggccgtgg gggagaagga
      541 tttcaacatc cttttccgct atctgctgcc gaattccatg gccccgatca tcgtgcagac
      601 gaccctccgg atggccacag ttctattgac cgcttcagga ttgagcttcc taggactggg
      661 ggtccaaccg cccacgcccg agtggggagc catgctctcc aatgcccggg cttacctgat
      721 caccgcccct catgtggcca cggttccggg cctggccatc atgatggtgg tgatggggtt
      781 caatcttttt ggggacgggt tgcgggattc acttgacccg aggcttaggg actagccaaa
      841 tgcggaatgc ggaataagag attgttactg gttgctagtg tcctgagtca gaaattcgta
      901 gcctaacttt ggcctggaga gggggcccgg tgcgcgcggc caaaatccgg cgggagaccc
      961 tagagttagc ccggaatccc tttacttccg cataactccg tgaggaagcc tgaaaacagg
     1021 tcttaaaaag atgaacccag cattcgccta ccgcaaagta gaggctgacg aggacttcgt
     1081 aagaaaaaca gcctattttg gcaagtgcat cgggtcccct ctccgggcct cggttggcct
     1141 gacaatttca atgaatttac gactcaggac actagttgct ggttactggc tggaacgaat
     1201 gggatcaaac gcgacatacg cgttgcgttc ggggaaggca ccctaaagag cccgaaccag
     1261 caacgaacag ccagcagctg gtaacgaata acctgaaacc agagactcga aactcgaaac
     1321 ccaaaacttc tttcaattat tcgcatggct acgcttctga gcgtcaaaga cctgagagtc
     1381 cacttcttta ccgccggccg gacggtccgg gccctggacg gggtgagctt cgaaattgaa
     1441 gagggcaaaa ccttcgggct ggtgggagaa accggctgcg ggaagtcggt aacggctctt
     1501 tccatcctcc gacttattcc gtttccgccg gggaagatca tgggagggga gattcatttt
     1561 cgggggaata accttctgtc cctttccgaa gaagaaatcc gctccatccg agggaaggaa
     1621 atttcaatga tcttccagga gcccatgact tccctgaatc cggtcttccg catcggcgat
     1681 cagatgtcgg aggtgatccg gcttcatcag gggctggacc gttccaaagc ctgggccaag
     1741 gcagaagaga tgttagagcg ggtccggatt cctgacaccc ggagggtggc caagcagttt
     1801 ccccatcaac tgagcggggg aatgaggcag agggtcatga tcgccatgga actctcctgc
     1861 catccctacc ttctcattgc cgacgaaccc agcaccgccc tggatgtcac catccaggcc
     1921 cagattctcc ggctcatcag ggagatgaaa gatgaacttc atacctcgat cctgctgatt
     1981 acccatgatc tgggagtgat tgccgagatg tgcgaccggg tgggggtcat gtacgccggt
     2041 tccattgtgg aagaggccgg ggtggaagag atctttgaat ccccgaggca tccctatacc
     2101 caagggcttt ggggagctat cccccggatc gatgacgaga aggagaacct ttcagtgatc
     2161 cccggaatgg ttccggattt gagcaatccc cctgccgggt gcagattcca tccccggtgc
     2221 gctcaccggt ccgatgtctg cgaacaaagc atcccgccgt tgtttgccgt ggcccccggt
     2281 cacctggttg cctgtttcct ccatagtgaa aggagagggc atggctgaac cgctgctgca
     2341 ggttcggggg ttgaaaaagc actttcccct cgcccgggga tggaggaaag aaaaggccct
     2401 ggtccgggca gtggacgggg tcgatttttc catccgccgg ggagaaacgc tgggactggt
     2461 aggagaatca ggctgcggga agaccaccgt gggaaggctg attctgcggc tgatcgaccc
     2521 tactgaaggg gaagttctgt ttgaggggaa aaacctggcc tctttggagg agaaggcgat
     2581 acatcccctg aggcggtttt tccagatcat ctttcaagat cccttcgctt ccctcaaccc
     2641 ccgcatgacg gttagcgaga ttgtgggccg gccgatggag gtccacggcc tggcggaaag
     2701 gtcccagagg gaggagaagg tggctaacct cctcaagtcc gtaggcctca tgagagagca
     2761 cctgggccgg tacccccatg agttttccgg ggggcagcgg cagcgaatcg ggattgccag
     2821 ggccctggcc acgcaaccct ctctgatcgt ggccgacgaa ccgacttcgg cccttgatgt
     2881 gtcggtgcag gcgcaaattt taaacctgct gaagaaactg cagagggatt tgggtctgac
     2941 ctatctcttc atctcccaca acatcggagt cgtccgccat atcagcgacc gggtggcggt
     3001 catgtacttg gggaaaattg tggaaatggc ggagaagaag attctttttc aatcccctgc
     3061 ccacccttac accaaagccc tgctggccgc ggtcccttcc cttgacccaa agagaaaaag
     3121 agaagaggtg atcctggaag gggatgtgcc cagccccatc aatcccccat gcgcctgccg
     3181 tttccatacg cgatgctcct atgccctgaa caaatgccgg caagaagaac ctctgttcca
     3241 ccagatcgaa gaaggccgat ggatttcctg ccacttgtat ccctgaaaga tagtctttct
     3301 gcttggccaa cgagtactga tttggctgag ggtgaacata attgcggaat tcggattgcg
     3361 gattgcgaaa taagaatgaa agaaagtaat tttatttaat gaaatgggga aaatatgttc
     3421 actttgatta aaggcgggac cctgtatggg ccgaagaaga tgggagtaaa agacatcctg
     3481 gtggtcggaa agaccatcgc caaaatttcc gaacgaatcg atctctcgga tgatttcaac
     3541 gtccaaattg tctgggctac cgggaaaatc atcacccccg gattcatcga tctccatgtg
     3601 cacatcctgg gcggaggcgg ggagggaggg cccaggacaa gaactccgga gatcactctc
     3661 tcccagatta ctcgggcagg agtcaccacg gttgtaggat gtctgggaac ggatgatgta
     3721 actcgaaaaa tcgaaactct cctggccaaa gccatgcaac tggaggaaga aggaatctcc
     3781 acctttattt attgcggctc ctaccagttt cccccgccga cgatcaccgg atgcgtccgg
     3841 ggagatattg ctctcattcc gaaagtgatc ggagtggggg agatcgccat ttctgatcac
     3901 cgctcctccc agccgacatt cgaagaactt acccgcattg ccgctgaggc gagggttggg
     3961 gggatgatcg ggggaaaacc cggcctggtc catctccaca tgggaagtgg ccctcgcagg
     4021 cttgatccga tcatccggat cgtcaaggag accgaaatcc ccataggcca gttccttccc
     4081 actcacctga ctcgaacccc agaactcctt gagcaggcga ttcaatttgc ccggttaggc
     4141 gggaacatag acttcacggt gaaaggaaag gatttatcct ttcccctcac cacccagcag
     4201 gccctccaga aagcgctgga tgcgggagtg tccatggatc agattaccct gagctccgac
     4261 tccaacggtt ccattcccat tttcgacgaa agaggtaatc ttattcggct ggccgtgggg
     4321 gatattcaga atttattcag agaatggagg ggcctagtcc gtgaaggctt tcctatcgag
     4381 gagatattga aactggtaac ggcaaacccg gcaaagagaa ttggagcgtt cgacagcaaa
     4441 ggaagcctgg ccgagggaaa agatgccgac ctgctaatct taaatcctga tttggaaatc
     4501 gattcagtca tggccaaagg aaggctgttg attcttcaaa atcaatttct agtcaaaggg
     4561 acctttgaga attgaaacgg ggtctcccct tttttcaaac tcctgcaagt ccgagtggcc
     4621 gggcgttgaa aggaagccga ggtgagtaag aaaaaccaaa cccttctgat cctgaccgtt
     4681 tcgatggccc tgggatacat gccctggtac aatttttccg cggtcctgaa atacctcgcc
     4741 gatgaatttc atctgacgac atccgacacc gggtggaccc tggcagcgtt ccaggctgga
     4801 tatgtgattg tggtgggggg cacggggtgg ctcggcgacc gggtgagtct caagaagatc
     4861 gtcctctggg ccaccctgat taccggggtg ttttccgcgc tctttgtctg gggagcaggg
     4921 gggaaagaga gcatcattgc cctgcggcta atcaccggtc ttgccgccgg ggcaatttac
     4981 gttcccgggc tggccctttt atcacaatgg ttttccccaa gggagcgggg aggagccctg
     5041 ggagcctata ccggagccct cgttgcctct tatgcggggg gatatctggt tgctggtcgc
     5101 ctggccgcta cctacggatg gagaaccgga gttttatgga cttccctccc ggcgattttg
     5161 gccgccttgc tcatttatct tcttgtgcgg gatgcgcctg cgtcggtgca aagcagcgga
     5221 agcggaaaga ggcagggaat ggcggagcgc gctcaggatt taagcggcag ggaaggtgga
     5281 gaaagtgaaa ttttggaagc tcctcccggg ggatttaaag gcccagctct cctatccgtc
     5341 agctacatgg ggcacatgtg ggagctctat gccttctggg gatggatcgg cccctttctc
     5401 gtttcctccg ccctagcggt tgggatgcct ccggatggag cggtgaaatg gggggcaacc
     5461 tttgcagcgg cgatcatcct gattggagcg ccggctacat ggggttgggg gatcgtggct
     5521 gataggaagg gaaggacctg ggcgattatg gtggccggcg ttcttagtgt aggagcggag
     5581 ttcttcgtcg ggtatctata tggacactcg ctttccattc tggtcttggc gggagcctgg
     5641 attggattct gggtaatcgc cgattcggcg atctacaagg caggcctaac cgaaatggtt
     5701 cttccccgga ttcggtccac cagtttgggt atacagtccg tggttggttt ttctgtcacc
     5761 atcatctccc ccatggtatt cggtaaaatc ctggagtact acaacggccc cgtggccgcc
     5821 gcgcaggcaa aggtctgggg cccggccttt gtggctctgg gtataggagg gatggtagcg
     5881 cccgcggccg cctggatgct ccgccgccag agacaggcca agctcatggc cggggggaaa
     5941 aaataaaatg aacccctccc agggatcgtt tcgctccttg cttttcgggt cattcctgtt
     6001 ttattcttag actaggcggt cttgtttttc tctgagattt ctggagtacg ccagtccatc
     6061 cggagatccc ggtagagatt atcgggttag agcagaggaa acgatcatca ggcccgccat
     6121 caccctaagg ccttgagggt gatcttgatt atgaaccatt ccatcaatcc ttactaaaat
     6181 tata
//
LOCUS       DASPRR010000292         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_871756, whole genome shotgun sequence.
ACCESSION   DASPRR010000292 DASPRR010000000
VERSION     DASPRR010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871756"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VEL68_11120"
     CDS             <1..288
                     /locus_tag="VEL68_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HXZ36559.1"
                     /translation="TELWRRGSVIASWLLDLCAMEFIKNSDLSNFAGSVSDSGEGRWT
                     LTAAIEEGSPAPVLSAALYQRFSSRGEDDFARKVLSAMRFGFGGHVERAGK"
     gene            350..1171
                     /gene="pgl"
                     /locus_tag="VEL68_11125"
     CDS             350..1171
                     /gene="pgl"
                     /locus_tag="VEL68_11125"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXZ36560.1"
                     /translation="MSPDSRGTEKPFLNVEIQVRADLDELSRAAASEVLRQAQRAVQA
                     KGLFSLALSGGSTPKSLYSLLADDSSVRAEFPWDKTHFFWGDERHVPPEHPESNYRMA
                     REAMLSKVPIPSENIHRINAGNPDAQRAAEEYEETLRAFFHLGAGEFPQFDLVLLGMG
                     ADGHTASLFPATAALGERKHLVVANWVGKFHVHRITMTLPVINRAAMVLFLVSGKEKA
                     ETLRRVLADRRGKVLFPCQLVRPTHGRLLWLVDQGAGGFPEGIPSIIISDQSPGP"
     gene            complement(1390..2184)
                     /locus_tag="VEL68_11130"
     CDS             complement(1390..2184)
                     /locus_tag="VEL68_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36561.1"
                     /translation="MRNWTKQAFQKHLQHRFSLRLHMCLILLATILSGVLFLKILLML
                     GVTDFRVRYPLSVLLSYLVFFACIRLWFFCISPNRSGKTNTIGGLDLPLPSDRSSGGG
                     GVPSFRSGGGQFSGAGASGSFEGDNAAIAETRLLANAEPTPAASASKGVSGDVGGVAD
                     ALGDDNVIVAVIVLVVLVATVLGSTIYVLYDAPTILSEAAFQGFLAASLIRRTRAMSY
                     QGWVGSVFRTTWKPFVVTVGVAFLSGVVLYSYFPEAVRLTDILWKR"
     gene            complement(2400..2528)
                     /locus_tag="VEL68_11135"
     CDS             complement(2400..2528)
                     /locus_tag="VEL68_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36562.1"
                     /translation="MDTKRLLPPIGIALLLTGCELVMDDNESGCSQILRILVLSHP"
     gene            complement(2551..>3971)
                     /locus_tag="VEL68_11140"
     CDS             complement(2551..>3971)
                     /locus_tag="VEL68_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXZ36563.1"
                     /translation="MVQAMLPPNTPLEQTQKVMEEVRKHFMEGEKDAAESIMTISGRS
                     FSGNGQNLGLAFVKLRDWDLRERPELKADAVAGRAMRAFAKLRNAMVFAFPPPAVVEL
                     GIAKGFDFQLLDRGGLGHAELMAARNQLLGIAAKDPRLTRVRPNGLEDGPEYRIDVDW
                     EKSGALGIPITSIHNTISAAFGSAYVNDFIQAGRVKRVYVQADAPYRMLPKDLEKLYV
                     LNTAGKMVPFSSFATGHWTSGSPKLERFNGFPSINIWGEPAPGRSSGEAMQDMQEFVS
                     RLPRGFGYDWTGLSYQERMASSQAPLLYAFSVFVIFLVLAALYESWPIPVSILLALPL
                     GAIGGVIGSTLRGLPNDVYFQIGLLTTLGLTTKNAILIVQFAKARVEEGMGLIEATLE
                     GAKLRLRPIVMTSLAFGFGVLPLAFATGAGAGAQKAIGTSVLGGVVTSTFLVTLFAPL
                     FYVLIEKAFGKNKKREEPRSIS"
BASE COUNT          902 a         1138 c         1142 g          789 t
ORIGIN      
        1 accgagctat ggcggcgggg gagcgtaatc gcatcgtggc ttctcgacct ctgtgccatg
       61 gagtttatca aaaattccga tctgtccaac tttgcgggga gcgtgtcgga ttccggggag
      121 gggcgatgga cccttactgc ggccattgaa gagggatcgc cggcccccgt gctgagcgcc
      181 gccctctatc agaggttcag ttcccgtggc gaggatgact ttgcccgaaa ggtgctctcc
      241 gccatgcgct tcggtttcgg ggggcacgtc gagcgggcgg gaaaatagag gctgtggagc
      301 tattggaacg cgacggccgg aggcggagta agaccggaag gtgaagggca tgagcccgga
      361 tagcagaggg accgaaaagc cattcttgaa tgtcgagatt caagtgcggg cggacctcga
      421 tgaactcagc agagccgcgg cttcggaggt cctccggcag gcgcagcggg cggtgcaggc
      481 caagggcctg ttttccttgg ccctgtccgg tggttcaacg cccaagtccc tctactcctt
      541 gctcgccgac gattcttctg tccgcgccga gtttccctgg gataagaccc actttttctg
      601 gggagatgaa cgccatgtgc cgcccgaaca ccccgaaagc aactaccgca tggcccggga
      661 agccatgctt tccaaggttc ccatcccgtc cgagaacatt catcgcatca acgccgggaa
      721 ccccgatgcc cagcgagcag ccgaagagta cgaagagacc cttcgggcct ttttccatct
      781 gggggctgga gaatttcccc agtttgatct tgtgctcctg gggatgggag cggatggcca
      841 taccgcctct ctcttcccgg caaccgcggc ccttggtgaa cggaaacatc tggtggtcgc
      901 caattgggtg gggaagtttc atgtccatcg aatcacgatg accctgccgg tcatcaatcg
      961 ggcggcgatg gttctgtttc tggtgagcgg caaagagaaa gcggaaaccc ttcggcgggt
     1021 gctggcagac cgaaggggga aagtcctttt cccttgccag ctcgtccgtc cgacccatgg
     1081 tagattgctt tggctggtgg atcagggtgc cggaggtttt ccagaaggga ttccgagcat
     1141 catcatttcg gatcagtcac caggcccctg accacgcgga ggattatccg agtgcagcat
     1201 agccgattta ccgctaaaga tggaagtcag cggtgggctg caggcctgtg cgtctacacg
     1261 cgatgttcag gaacatattt tagggggttg aaaccactaa cccgaaagca tccatcgcga
     1321 tcaggaccca cataacatga tggtcagcag cgaggcgcgc ccttcggcca cctccggtcc
     1381 acctactcgt tatctcttcc aaagaatgtc cgtcagtcta acagcctcag gaaaatagct
     1441 atacaaaact acaccggaaa ggaaggccac ccccactgtc actacgaacg gtttccatgt
     1501 cgttctgaag acactcccaa cccatccttg gtagctcatc gctcgggttc gccgaataag
     1561 ggaggctgca agaaaaccct ggaaagctgc ttcggaaaga attgtgggtg cgtcatagag
     1621 aacgtagatg gtcgaaccta agactgtagc gaccaataca accagaacga taaccgccac
     1681 aatgacatta tcgtccccca aggcatccgc gactccgcct acatccccac tcacgccttt
     1741 agatgcgctg gccgcaggag ttggctcagc attggccaaa agtcttgttt cggcaatggc
     1801 agcattgtcg ccctcaaaag aaccggaggc tccagcgccc gagaactgac cgccgccact
     1861 gcgaaaggat gggactcctc ctccaccaga gcttcgatcc gaaggcaaag gaagatcgag
     1921 tccgccaatc gtattcgtct tgcccgaccg attgggagaa atacaaaaaa accacagtct
     1981 tatgcaggcg aagaaaacca gatacgatag caaaacggac aaaggatacc gaaccctgaa
     2041 atcagtaacc ccaagcatca acaatatctt taagaataat acgcccgaaa gaatagtcgc
     2101 caggaggatg aggcacatgt gtaggcgcag agagaatcga tgttgtagat gcttctgaaa
     2161 tgcctgcttt gtccagtttc tcatcgtccc attcctgggc taacaaggat catccagtct
     2221 ggatacaacc gcaaaggcgt tgaattcaag ctatcgtgcc agtttccgaa cctgcaaagt
     2281 tttgccaatt attgcagaaa tggtttaatt tataaacccc tttataatct agcaacataa
     2341 cagatttctt ttatccaaaa aaattagcat atgaaaattt tatgattcca gaaacgtatt
     2401 caaggatgag aaagcaccag gatacgaaga atttgggaac aaccactctc gttgtcgtcc
     2461 atcaccaact cgcagccggt cagcaagagg gcaattccaa tggggggtag aagccgcttt
     2521 gtatccatgt tcctgatctc cagaaggctg ttaggagatg ctcctgggct cctcacgctt
     2581 cttgtttttg ccgaaggcct tttcgatcaa cacgtaaaag aggggcgcaa acaaggtcac
     2641 caggaaggtg gaggtgacca ccccgcccaa cacgctcgtg ccgatggcct tttgggcgcc
     2701 cgcgcccgcc ccggtcgcaa aagccagcgg caggacccca aatccgaagg cgagcgaggt
     2761 cataacgatg ggacgcagcc ttaatttcgc cccttccagg gtcgcctcga tcagccccat
     2821 cccttcttcc acccgggcct tggcgaactg gacgattagg atggcgttct tggtggtcag
     2881 gccgagcgtg gtcaacaggc cgatctggaa atagacgtca ttgggcagcc cgcggagcgt
     2941 cgagccgatc accccgccga tggcccccag gggcagggcc agcaggatag agacggggat
     3001 gggccagctt tcgtacaggg ccgctaagac taggaaaatc acgaagaccg aaaaggcgta
     3061 gagcaggggc gcctgggaac tggccatccg ctcctggtag gagagcccgg tccagtcata
     3121 gccgaacccc cggggcaggc gggagacgaa ctcctgcatg tcctgcatgg cttcgccgga
     3181 actcctcccc ggcgcaggct cgccccagat gttgatggag ggaaagccgt tgaaacgctc
     3241 cagcttcgga gaaccggaag tccagtggcc ggtggcaaag gaagagaagg gaaccatttt
     3301 cccggcggtg ttgaggacgt agagcttttc caggtccttg ggtagcatgc ggtagggggc
     3361 gtccgcctgg acgtacaccc gttttaccct tccggcctgg atgaagtcgt tcacataggc
     3421 actgccgaag gccgcggata tggtgttgtg tatggaggtg atggggatcc ccagggcgcc
     3481 ggacttttcc caatccacgt cgatccggta ttcaggcccg tcctccaggc cgttgggccg
     3541 gacccgggtc aatctcgggt ccttcgccgc aatgccgaga agctggttgc gggccgccat
     3601 cagttcggcg tggcccaggc ctccgcggtc caacagctgg aaatcgaacc ccttggcgat
     3661 accgagttcg acgaccgcgg gcggcggaaa ggcaaagacc atggcgttgc ggagcttcgc
     3721 aaaggccctc atcgctctcc cggccacggc gtcggccttc aactccggcc gttcgcggag
     3781 gtcccagtcc ctgagcttga caaaggccag gcccaggttc tgcccgttgc ccgagaagct
     3841 ccggccggaa atcgtcatga tggattcggc cgcatctttc tccccctcca taaaatgctt
     3901 ccggacctcc tccatgacct tctgcgtctg ctccaggggg gtgttcggcg gaagcatggc
     3961 ctggaccatg a
//
LOCUS       DASPRR010000293         3780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_219084, whole genome shotgun sequence.
ACCESSION   DASPRR010000293 DASPRR010000000
VERSION     DASPRR010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219084"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VEL68_11145"
     CDS             complement(<1..377)
                     /locus_tag="VEL68_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36564.1"
                     /translation="MKDCAQCYGKEILEGLMTCPGGHEFAISRGILRALGKKANGDPG
                     EGEEFFHSLKRHGGVKATFDREWQMVDAGREVYGHSPEAEEEDFFRRLCVEESFLRGK
                     LILDVGCGIGRLADRLTQKADEVV"
     gene            complement(536..1216)
                     /locus_tag="VEL68_11150"
     CDS             complement(536..1216)
                     /locus_tag="VEL68_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ36565.1"
                     /translation="MNSFRVQRIPPPLLKKFSEGCYVDSPVYFDAPGWVRWLNWAKLE
                     WLLAMVPHEESLRVLDFACGNGVMFPTWEGHFLRTVGIDLQVTAADRVRKYFHLERVC
                     LARSEGGRLPFREESFDLAFAASSLEHFADLKLPLEEVRRVLRRGGQMVFLCPNENEF
                     YGWGRRLAGYEKPPDHYHSADDVMKAVGSFFHLAEVSWFPRWVPPFFGIYKMGRAVKS
                     RKAEGGRS"
     gene            complement(1213..1983)
                     /locus_tag="VEL68_11155"
     CDS             complement(1213..1983)
                     /locus_tag="VEL68_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ36566.1"
                     /translation="MILGQKITIVFPAYNAERTLEKTYQALDRQVVDDIILVDDCSQD
                     RTVEIARHLGLHVICHEQNRGYGANQKSCYRAAMERGAEVVVMVHPDYQYAPELVPAM
                     GWLIACGLFDVVLGSRILGTGALQGGMPRYKYIANRVLTGFQNFLVSHKLSEYHTGYR
                     AFSRKVLEGLPLEENSNDFVFDNQMLAQSIHFGYRIGEVTCPARYTAESSSINFHRSV
                     IYGMGVLKTSFQFRLNRMGIFPSRLFAPDGRRLKGEPG"
     gene            complement(1980..3407)
                     /locus_tag="VEL68_11160"
     CDS             complement(1980..3407)
                     /locus_tag="VEL68_11160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ36567.1"
                     /translation="MKVLLVNPPYLPEERYGNLAAFGPTNEPLGLAYIAGSLAEAGVK
                     VKIADAPALRMSVEEIGNWAVRDRFDLVGVTMLTPMYHRSLEVLRQVRKSSPAIRTVV
                     GGPHPTVLPEQTLQEIPELDFAVIGEGEITSVELVEALEGKRGIDSVAGLAFRKDGRV
                     VANPLRPPMRNLDQLPLPARHLLPMDAYRMTRSRTQTEHSYTVSVARGCPFRCAYCFR
                     TFGRAFRPHSPARIIQEISLLVDQYGAKEINLEADTITLNRSFILDLCRALGESGLSR
                     KVQWTCESRVDTVDEEILRAMKEAGCWQISYGVESGSQRLLDLIEKGITLEQVEKVFA
                     LTKKLGITIRAFFMLGLPTETREESWQTIRLARKLNAEWSQFTITVPFPGTKLYELAQ
                     AEGALRSARWADFKTYGGWTEGDLVYVTKGRSAAELKALQKKALKSFYLRPAVFWRFL
                     KSIRSYEQFREAARGFFTLVKTRTG"
     gene            complement(3412..>3780)
                     /locus_tag="VEL68_11165"
     CDS             complement(3412..>3780)
                     /locus_tag="VEL68_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36568.1"
                     /translation="PWNNLSLWETCCPEDFGLMGEAYLSFGRLSRAVYLSDTGRGWNS
                     VENLRDRFSPEGPAMPGPFSSTGELIRFLRGNPFPMVYLLVHPNRWTSGRGSWLIQWG
                     EDASVNWLKRKLKRHASARS"
BASE COUNT          793 a         1163 c         1055 g          769 t
ORIGIN      
        1 actacctcgt ccgccttctg ggtgaggcgg tcggcgagcc gaccgatccc gcagcctacg
       61 tcgagaatga gcttcccccg gagaaaactc tcttccacac acaaccgccg gaaaaaatct
      121 tcctcttcgg cctctgggga atgaccgtag acctcccggc ccgcatcgac catctgccat
      181 tcccggtcaa aggtggcctt caccccccca tggcgcttta gtgaatggaa aaattcttcg
      241 ccctcccctg ggtccccgtt cgcctttttt cccagggccc gcagaatccc cctggaaatg
      301 gcgaattcat gccctcccgg gcaagtcatc aggccctcga gaatttcttt cccgtaacat
      361 tgggcgcaat ctttcacggt gattcctgaa gaccctcttt cttctctcca caagcaacgg
      421 tcttggcatg ggatggagga gggctgaaaa gagggggcgt cttcttcctt caggacccga
      481 aggaaaaaac tttccccgca aatcgggcaa gccagaagat cgaaaagcct tctcttcaag
      541 acctccctcc ctcagccttt ctcgatttga ccgcccttcc catcttgtag atgccaaaga
      601 agggaggaac ccaccgggga aaccaggata cttccgccag atggaaaaag gaccctacgg
      661 ccttcatgac atcgtcggct gaatggtagt gatcgggtgg cttttcgtat ccggccaggc
      721 gacgccccca accatagaac tcgttctcat tggggcagag gaagaccatc tgccctcccc
      781 ggcgcaagac ccggcgaacc tcctccaggg ggagcttcag gtctgcaaag tgctccaggc
      841 tgctcgccgc aaaggccagg tcaaaggact cctcccggaa gggaagcctc cccccttcgg
      901 agcgtgcgag gcaaactctt tccagatgaa aatatttgcg tacccggtcc gcggcggtta
      961 cttggaggtc aatccccaca gtccgcagaa aatgcccctc ccaggtgggg aacatgactc
     1021 catttccaca ggcaaaatcc aggactcgga ggctctcttc atggggaacc atggccaaga
     1081 gccattccag cttggcccag ttgagccaac gaacccaccc tggggcgtcg aaatagaccg
     1141 gcgagtctac atagcagccc tcgctgaact tcttcaagag gggaggagga atcctctgta
     1201 cccgaaaaga gttcaacccg gttccccctt taggcggcgc ccatcggggg cgaagagacg
     1261 gctggggaaa attcccatgc ggttcaagcg gaactggaag gaggtcttca gcacgcccat
     1321 tccatagatc acgctgcggt ggaagttgat ggaagaggat tcagccgtgt accgcgcggg
     1381 acaggtgacc tcgccgatgc ggtacccaaa atggatgctc tgggccagca tctggttgtc
     1441 gaagacaaag tcattggagt tctcctccag gggtagaccc tccagaacct ttcgggagaa
     1501 ggcccggtac cctgtgtggt attccgatag cttgtggctc accagaaagt tttggaaccc
     1561 tgtcagaacc cggttggcta tatatttgta ccgcggcatc ccgccttgca gggctccggt
     1621 ccccaggatg cgcgatccca gaaccacatc gaataacccg caggcaatca gccatcccat
     1681 ggccggcacc agctctgggg cgtactggta atcgggatgg accatgacca ccacctccgc
     1741 cccccgctcc atggccgccc ggtagcagga tttttgattc gctccatacc ccctgttctg
     1801 ttcgtggcag atcacatgga gaccgaggtg ccgggcgatc tctacggttc ggtcctggct
     1861 gcagtcatcg accaaaataa tatcgtccac cacctgtcgg tccagggctt ggtaggtctt
     1921 ctccagggtc cgttcggcat tgtaggccgg aaagacaatg gttatttttt gccccagaat
     1981 catcccgttc tcgtcttcac gagggtgaaa aatccccggg ccgcctcacg gaactgctcg
     2041 tacgagcgga tggatttcag gaagcgccag aagaccgccg gacggaggta aaaggattta
     2101 agggcctttt tctgtaacgc tttcagctcg gctgcggacc ggcccttggt tacgtacacc
     2161 aggtcgcctt cggtccaccc tccataggtc ttgaaatccg cccagcgggc tgaccgcagc
     2221 gccccttcag cctgagctag ctcgtagagt ttggttcccg gaaaggggac cgtgatggtg
     2281 aactgggacc actcggcatt cagcttgcga gccaaacgga tcgtctgcca gctctcttcc
     2341 cgggtctctg taggcaggcc cagcatgaag aaggcgcgga tggtgattcc cagtttcttg
     2401 gttagggcaa agaccttctc cacctgctcc agggtgattc ctttctcaat gaggtcgaga
     2461 aggcgttggg agccgctctc cactccgtaa ctgatctgcc agcagccggc ctccttcatg
     2521 gcccggagga tctcttcgtc cacggtgtcc acccggctct cgcaagtcca ttggactttc
     2581 cgggaaaggc cgctctcccc cagggcacgg cagaggtcga ggatgaagga acgattgagg
     2641 gtgatcgtgt ccgcttccag gttgatctcc ttggctccat actgatccac cagaagggaa
     2701 atttcctgaa tgatgcgggc cggcgagtgg gggcgaaagg cccgtccaaa ggttcgaaaa
     2761 cagtaggcgc agcggaaagg acatccccgg gccacgctta ctgtgtaact atgttccgtc
     2821 tgggtccggc tgcgggtcat ccgataggcg tccatgggga ggaggtggcg ggcgggcagc
     2881 ggaagttggt ccaaattcct catgggcggg cgaagggggt tggcaactac cctgccgtcc
     2941 ttccggaagg ccaatccagc cacggaatca atacccctct tcccttccaa cgcctccact
     3001 agctctaccg acgtaatctc cccttccccg atgactgcga aatccagctc cgggatctcc
     3061 tgcagggttt gctccggcag gactgtggga tgaggccccc ccacaacggt tcggatcgct
     3121 ggagagcttt ttcgcacctg gcgcaggact tccaggctgc gatggtacat gggagtgagc
     3181 atggtcaccc ccaccaggtc gaaccggtcc cgcacggccc agttgccgat ttcctcaacg
     3241 ctcatccgca atgccggggc atccgcaatc ttgaccttta ccccggcctc tgccaggctg
     3301 ccggcaatgt aggccagccc caggggctca ttggtcggac cgaaggcggc gaggttgcca
     3361 tagcgttctt cgggtaaata aggaggattg accaaaagga ctttcattct ttcaactccg
     3421 agccgaagcg tgtctcttga gctttcgctt cagccagtta acggaggcat cttcccccca
     3481 ctggataagc caggagcctc tccccgaagt ccagcggttg gggtgaacca gcagatagac
     3541 catcggaaag gggtttcccc ttagaaaacg gatcaattcc cccgtagagg aaaagggacc
     3601 cggcatggcc ggcccttcag gggaaaaccg gtcccgcagg ttttctaccg agttccagcc
     3661 cctccccgtg tcgctcaggt agacagccct ggaaaggcgg ccgaaagaaa ggtaggcctc
     3721 tcccataagg ccaaagtctt ccggacagca ggtttcccag agagataggt tgttccaggg
//
LOCUS       DASPRR010000294         5373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_714883, whole genome shotgun sequence.
ACCESSION   DASPRR010000294 DASPRR010000000
VERSION     DASPRR010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714883"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..2134)
                     /gene="pbpC"
                     /locus_tag="VEL68_11170"
     CDS             complement(44..2134)
                     /gene="pbpC"
                     /locus_tag="VEL68_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HXZ36569.1"
                     /translation="MPSYPEVRKVYMKSDSLLLDRHGEIIHELRMDFKQRRLDWTPLR
                     DISPALKEAVIAAEDRRFYAHSGIDLQALGAALFQGLTSSGMRGASTISMQLVALLER
                     DLQPGKGRKSIWQKGKQVLAAWELERVWSKDEILEAYLNLITFRGELQGISAASRGLF
                     GKDPHGLDRSEALLLASLIRAPGASYADLTKRANHLAESLRWPVDPKETSSRGSQVFL
                     GPNLLRPQTALAPHLARQLLKDQPPGTSLGCTLEGEVQRFALDRLAHQLLPLRSQNVA
                     NGAVLVVENRTGEILAYVSYSSDPALSGFVDGVKAKRQAGSTLKPFLYGLAMDQRLLT
                     PASLLDDSPLDVPVFSGIYHPRNYESQFQGLVPVRIALASSLNIPAVRVISLVGLEDF
                     LNRLRALGFRDLNEAGDFYGPALALGSADVSLLELVNAYRTLANGGEWSELRSAFDGG
                     LNPPRKRVFSSEAAFLISDILSDREARSSTFGLENPLSTRYWSAVKTGTSKDMRDNWC
                     IGYSDKYTVGVWVGNFTGEPMWNVSGISGAAPVWVEVMNRLHRDKGNPGKEPPPRLVK
                     AHIEFPQGLHPPCEEWFIRGTEPALLEAKSLFLHQRILYPPPGSIFALDPDIPRELQK
                     ILFTLQAPQQKVTWVLNGQPLPSLGKATPWSPEAGKFHLDLRDDEGRIIDSVRFEVRG
                     SNMEGYSEDGESPG"
     gene            complement(2461..>5373)
                     /locus_tag="VEL68_11175"
     CDS             complement(2461..>5373)
                     /locus_tag="VEL68_11175"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HXZ36570.1"
                     /translation="QRKILTHRKRIAGGFYSYDHTEETKKVASLCSGKTDSKGLMICE
                     TRSPVSGNLIVQAQTSDPSARRAAAQQEIWVAEKEDWWFAAGDHDRIDLLPEKKRYEP
                     GETAVFQVRMPFREATALVTVEREGVMETWVRKISGKKPVIEVPVKGNYAPNVFVSVF
                     PIRGRVVDVKPTSLVDLGRPAYKLGIAEINVGWREHELKVDVSADRKVYRVRQKARVK
                     VAVKTWDGKAPPAGSEVAVAAVDQGLLELMPNKSWEILPAMMGRRGYGVHTATAQGQV
                     IGRRHFGLKALPQGGGGGKQTTRELFDTLLLWKGRIPLDANGEGSLEVPLNDSLTSFQ
                     IVAVATGGMGLFGNGSTIIQSTQDLMVFSGLPPLVREGDRFQAEFTLRNATNRNMAVD
                     LSAKVGGIAGPFKPISASLKPGEAKEVGWEATVPRGLETLRWEVEAQERGSPEKDRLR
                     ISQKIAPAVPVRTFQATITQLEKDFQLPVERPVDAFPGRGGVNVNFRPRLAESMNGVN
                     EYMRRYPYGCMEQRISIAVSLREAKRWKELMAQLPSYQDGDGLVKYFPGMRLGDETLT
                     SYILAIGNEAGWSIPEEPKERMGAGLRKFIEGRIVRGSPLPTVDLALRKLTAMEALSR
                     SGNFQGKLLGSISIEPNLWPTSAVIDWLNLLRNSRDIPNRAERLREGENILSSRLNFQ
                     GTVMSFSTERSDCLWWLMVSGDVNAVRTVLALLRSENWKEDMPRLVQGALARQRQGKW
                     DLTLANAWGVLAMEKFSRAFEAVAVSGITRAELSNQTQSIDWKASPKGKVSAFPWPEE
                     KGNLTIAHQGSGKPWVTVQSLAAIPLKEPLSSGYKIQKSVSPVQRKASNGWSRGDILR
                     VRLEMEAQADQTWVVVSDPIPAGSSILGSGLGRDSQLLTQEEKKEGWVWPAYEERSFE
                     AFRAYYRYVPKGSWTVEYTIRLNQSGLFQLPTTRTESLYFPEMFGEIPNRNFEVQP"
BASE COUNT         1066 a         1621 c         1548 g         1138 t
ORIGIN      
        1 gtcctttcgc atagcgacct ccttttcgaa aaaatctttc ccgttaccca ggggattcgc
       61 catcttcact gtacccttcc atattggacc ctctgacttc aaagcgaacc gaatcgatga
      121 tccggccctc gtcatccctc aggtccagat ggaacttgcc cgcctccgga ctccaggggg
      181 ttgcctttcc gagggagggc aaaggttggc cattaagcac ccaggtgact ttttgctgag
      241 gggcctggag agtaaagagg atcttctgca gttcgcgggg tatatcgggg tccagggcaa
      301 agatgctccc gggcggggga tagaggatcc gttgatgaag aaagaggctt tttgcctcca
      361 gaagggcagg ttcagttcct cgaatgaacc attcttcgca cggggggtgg agtccttggg
      421 ggaattcgat atgtgccttt accagtcgag ggggcggctc tttcccggga ttccccttat
      481 cgcggtgaag ccggttcatc acctccaccc acaccggagc tgctccgctg atcccgctca
      541 cgttccacat gggctccccg gtgaaatttc ccacccacac gcccaccgtg tatttatccg
      601 aatatccgat gcaccagttg tcgcgcatgt ctttgctggt cccggttttc accgccgacc
      661 agtaccgggt cgacagggga ttttccaggc cgaaggttga ggaacgggcc tcccggtcgg
      721 agaggatgtc ggaaatcaga aaggctgcct cggaggaaaa gacccgcttt ctcggagggt
      781 tcaatccccc atcgaacgcg gagcggagtt cactccactc acctccgttg gccagagtcc
      841 ggtaggcgtt gaccagttcc aggaggctca cgtcggcgga acccagggcc agcgccggcc
      901 cgtagaaatc ccccgcttcg ttgaggtccc ggaatcccag ggctcgcagt ctgttcagaa
      961 aatcttccag ccctaccagg gatatgaccc gcaccgcggg gatattcaat gaagaagcca
     1021 gggcgatgcg gaccgggacc agaccctgga actggctctc gtaattccgg ggatgataga
     1081 tcccgctgaa aaccggaaca tccaggggag agtcatccag aagggaagcc ggagtcagga
     1141 ggcgctggtc catagcgagg ccgtagagga aaggtttcaa agtggacccc gcctgtctct
     1201 tggccttgac cccatcgaca aaaccggaga gggccgggtc gctgctgtaa ctcacgtagg
     1261 ccaggatttc cccagtcctg ttctccacca cgagaacagc gccattggcg acattctgcg
     1321 accgtaaagg gagaagctgg tgagccagcc ggtccagggc gaaacgctga acctcaccct
     1381 ccagggtaca gcccaaagaa gtccccggag gttggtcctt cagcaactgc ctggccaaat
     1441 gaggggccag ggcggtttgg gggcgaagca ggttggggcc gaggaaaacc tggcttcccc
     1501 gggaagaagt ctccttgggg tccaccggcc accttaaaga ctcggcaaga tgattcgccc
     1561 ttttggtcaa gtccgcatag gaggcccccg gagcgcgtat cagggaggct agaaggaggg
     1621 cctccgaccg gtccaggccg tgggggtctt tgccgaacag cccgcgggag gctgcggaaa
     1681 tcccctgaag ttctccacgg aaggtgatca ggttcaagta ggcttcgagg atttcatcct
     1741 tcgaccatac gcgctccaat tcccatgcgg ccaggacctg ttttcccttc tgccagatcg
     1801 atttccttcc ctttcctggc tgcaagtccc tttccagcag ggcgaccagt tgcatcgaga
     1861 tcgtactcgc cccccgcatg ccggaagaag tcaggccctg gaatagagcg gcgccgagag
     1921 cctggaggtc gattccggaa tgggcgtaaa aacggcgatc ttcagcggca attacggcct
     1981 ccttcaaagc tggggaaata tcccttaggg gcgtccagtc caggcggcgc tgtttaaaat
     2041 ccattcgcag ttcgtggatg atttctccat gccggtcgag caaaagggag tcggatttca
     2101 tgtaaacctt gcgcacttcg gggtaggaag gcatagcatg gaccgaagga atcgacaaaa
     2161 ttgaagagca gagccgtgta aaatcccccc accccccctt tgccaaaggg gggcaggcgg
     2221 gatttctgcg cccatccggc agagcaacga tgaattcccc gccgataagg agcgatgcaa
     2281 gaacgataag gaaaaggagc ctcctgcaaa agttgcttcc tctgtcattc cggcgaaagc
     2341 cggaatccag aatcctttga aaatcctgga ttccgagtcg cgcttcgctt gcccggaatg
     2401 acaacttccc cagtttccga gaattttgca ggaacctcag aaggatagaa aatcttttca
     2461 ctatggttga acctcaaaat tccggttggg aatttctccg aacatttcgg ggaagtagag
     2521 cgattccgtc cgggtggtgg ggagctggaa aagcccgctc tggttcagcc ggatcgtgta
     2581 ttcaacggtc caacttcctt tcggcacata ccggtagtaa gccctaaagg cctcgaaaga
     2641 cctctcttcg taggccggcc atacccagcc ttcctttttc tcttcctggg tcagaagctg
     2701 ggaatccctt cccaggccgc tccccaggat cgaagaccct gcggggatgg gatcgcttac
     2761 cacgacccag gtctgatccg cctgggcttc catctccaag cggacccgca ggatgtcgcc
     2821 acggctccag ccgttcgagg ctttccgctg gacgggactc acggattttt gaatcttgta
     2881 gccgctggag agaggctctt tcaggggaat ggcggccaga ctctggacgg ttacccatgg
     2941 ttttcccgac ccctgatggg cgatcgtcag gtttcccttt tcttctggcc agggaaaggc
     3001 ggaaaccttt cccttgggcg aagccttcca gtcgatggac tgagtctggt tcgatagctc
     3061 agccctggtg atcccggaga cggcaaccgc ctcgaaggcc cgggagaatt tctccattgc
     3121 caggactccc caggcattgg ccagggtgag gtcccatttt ccctgtctct gcctggccag
     3181 ggctccctgg accagccggg gcatgtcctc tttccaattc tcggaccgga gcagagccag
     3241 aaccgtccgc accgcgttca catccccgga aaccatgagc caccaaaggc agtcggacct
     3301 ttcggtggag aagctcatca ccgttccctg gaaattcagc cgggaagaga ggatattctc
     3361 cccttccctc agccgctctg cccgattggg aatatcccgc gaattccgaa ggaggttcag
     3421 ccagtcgata accgcggagg tgggccagag attgggttcg atggaaatgg agcccaggag
     3481 ttttccctgg aaattcccgc tcctggagag agcctccatg gctgtcagtt ttcgcagggc
     3541 gaggtcgacc gtgggcaggg gggagccgcg gacgatcctg ccctcgataa atttccgcag
     3601 acccgctccc atcctctctt tgggttcttc cgggatggac catccggcct cattcccgat
     3661 ggccagaata taagaggtga gcgtttcatc gccaagcctc attccgggga aatatttcac
     3721 cagaccgtcc ccgtcctggt atgaaggaag ctgggccatg agttccttcc acctctttgc
     3781 ctcccggaga gagacggcga tggaaatcct ctgctccatg cacccgtaag gatatcgcct
     3841 catgtattcg ttcactccgt tcatgctctc ggccaggcgg ggccggaaat tcacgttcac
     3901 cccgccgcgg ccggggaatg catcgaccgg ccgctccacg ggaagctgga aatccttttc
     3961 caactgggtg atggtggcct gaaaagttct gaccggaacg gccggggcga tcttctggct
     4021 gattctcagc cggtcctttt ccggggatcc cctctcctgc gcctctactt cccaccggag
     4081 tgtttccagt ccccggggaa cggtggcctc ccagcccact tcttttgcct ctccgggttt
     4141 gagggaggcg gatatgggtt tgaatgggcc ggcaatcccc ccaaccttag cggaaaggtc
     4201 tactgccatg ttccggttgg tggcgtttcg cagggtaaac tcggcctgga accggtctcc
     4261 ttccctgacc aggggaggaa ggccggaaaa gaccatcagg tcctgggtgg attggatgat
     4321 ggtggaccca ttgccgaaga ggcccattcc tccagtggcc acggccacga tctggaagct
     4381 ggtgagggag tcgttgaggg ggacttccag ggagccttcg ccgtttgcat ccagcggtat
     4441 gcgacccttc cataaaagaa gggtatcgaa aagctcgcgg gtagtctgtt ttcctccccc
     4501 tccaccctgc ggcagagctt tcagtccaaa gtgacgtcgc ccgatgacct ggccctgagc
     4561 cgttgccgtg tgcaccccgt accctcttcg tcccatcata gccggcagta tttcccagct
     4621 cttgttgggc atgagctcca acaggccctg gtccacggcg gccaccgcta cttcgctccc
     4681 ggcgggaggg gcttttccgt cccaggtttt gaccgccact tttaccctgg ccttctgccg
     4741 cacgcggtac actttccggt ccgcggatac atccaccttc agctcgtgtt ccctccagcc
     4801 tacgttgatt tcggcaatgc ccagcttgta ggccggcctc cccaggtcca ccagggaggt
     4861 gggctttacg tccaccacac gtccccggat gggaaaaacg gagacaaaaa cattgggagc
     4921 gtagttccct ttgaccggca cctcgatcac cggctttttc ccggagattt ttcggaccca
     4981 cgtttccatg actccttccc gctccaccgt aaccagagcg gtggcctcgc gaaaaggcat
     5041 gcggacctga aaaaccgctg tctcccccgg ctcgtaacgt ttcttctcgg ggagaaggtc
     5101 gatccggtcg tggtcccccg cggcaaacca ccagtcctcc ttttccgcga cccagatttc
     5161 ctgctgggcc gcggcgcgac gggccgaggg atcggaggtc tgggcctgaa cgatcaggtt
     5221 cccggatacg ggcgatcggg tttcacagat catgaggccc ttggaatccg tctttccgga
     5281 acaaagggag gcgaccttct tggtctcctc ggtgtggtcg taggagtaaa accccccggc
     5341 aatgcgcttc cgatgggtga gaattttccg ctg
//
LOCUS       DASPRR010000295        19512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2003108, whole genome shotgun sequence.
ACCESSION   DASPRR010000295 DASPRR010000000
VERSION     DASPRR010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19512)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19512
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003108"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..582
                     /locus_tag="VEL68_11180"
     CDS             253..582
                     /locus_tag="VEL68_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ36571.1"
                     /translation="MRFPDFIEKLPKADLPVPEIEGFLLQAEKHQVAFLRSEKDMLMP
                     EHSHAAQWEIPLEGSAELTMDGQVKVMRPGQPIYIPAGVPHSGKVKGPYAAIIVFDAP
                     ARYKVKK"
     gene            complement(980..1468)
                     /locus_tag="VEL68_11185"
     CDS             complement(980..1468)
                     /locus_tag="VEL68_11185"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HXZ36572.1"
                     /translation="MAEHILILIVTFILMLIGLLGSVLPGLPGAPLILAGALVYGWYT
                     GFATVTWGVLAVLLVLMLLSYFLEFFASMLGVKKMGGSGWGMAGALLGMIVGTVIGGL
                     FGLLIGPFVGAFLFELVKQKDLQASLKSGIGTLVGFLAGTIGKLIIAVIMTAIFLFKI
                     IA"
     gene            1828..3066
                     /locus_tag="VEL68_11190"
     CDS             1828..3066
                     /locus_tag="VEL68_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015584979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HXZ36573.1"
                     /translation="MEKGNLRVNAKRLRATIEELARIGGTAGGGMHRLALTDEDKQAR
                     DLFVQWLKELGLEITVDEMGNIFGKRKGRSPQLAMVLSGSHLDTQPKGGRFDGILGVT
                     GALEALRTIHENRIDTERSIAIVDWTDEEGTRFAPAMLGSGVWAGHFDRDWAYARADL
                     EGKSFGEALERIGYKGKAPARKWPVHAYYEFHIEQGPVLEKMGKRIGLPKGILGITWF
                     DVYVEGQANQAGPTPMNSRKDALVAAAEMILTVSDLPAKMGGDLVGTVGEIQNFPNSR
                     NIIPDRVRFTVDIRSWDDPFKNQAAEAIKKEFFQIGQKRGCSVRMEMIWDILHTEFAS
                     NLVGRVEKVASLLGYSNYSMVSGAGHDAAYVCRVAPTAMIFVPSIGGRSHVEVERTGW
                     EDCEAGANVLLHCLLQSANE"
     gene            complement(3864..4802)
                     /locus_tag="VEL68_11195"
     CDS             complement(3864..4802)
                     /locus_tag="VEL68_11195"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXZ36574.1"
                     /translation="MRLEQPSNPKEMVFNQKPLSTGKKRAIIDQVIQQIEHLPTLPQV
                     VMRVLSMAESPKISAVELSRVLDQSLAAKVLRVANSAYYGGKGRKINSIHHAIMMIGF
                     ESVKEIILTTSFFHTLHDVRDVQSLQPLWQHSLQCALISKRLAWIYRYDSLDEAYLSG
                     LTHDIGRLVIQQFLSEQFNEIEERKKSGMEWLQAEKEVLETTHAEIGGKVAERWAFSP
                     NLIDVIGHHHDCSWTVNPTLGKILHFADKFTFGEMNFFNMLESFSREGMNYPSTWDSS
                     DLASVEQILQEEMGKAQSMFHFVMGRNPQTDDSLSS"
     gene            4982..6304
                     /gene="miaB"
                     /locus_tag="VEL68_11200"
     CDS             4982..6304
                     /gene="miaB"
                     /locus_tag="VEL68_11200"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXZ36575.1"
                     /translation="MNPRKFFIQTFGCQMNVYDSERIAQELTARDYRQVNDPARADLI
                     LVNTCSVREKPEQKVYSALGRFRRLKKKNPKLIIGVGGCVAQQEGQGLLERVPFLDFV
                     LGTKELCRIHEILDDLEKSGERGCAVNLDGRIDPYRGLALSPDPGRPTSFISIMQGCD
                     NFCSFCVVPFVRGREVSRPSKDLMKEIGVLSSQGVKEVTLLGQNVNSYGKQTPGEPGF
                     VELLERVEKIPGIERVRFTTSHPKDLSSELIQAFGRLTKLCEHIHLPLQSGSNRILKR
                     MNRGYTREEYEEKVLNLRQTCPEISITSDIIVGFPGEEERDHQETLEMIRKIQFDDLF
                     SFRYSDRPHTRASLFPDKIAEELKQRRLSELQAAQKDITQRKNQAWEGRDVEVLTEGP
                     SKAKAEERTGRTRTNRIVNFPGDSVPIGALVRVRVWHALAHSLRGELL"
     gene            6449..7102
                     /locus_tag="VEL68_11205"
     CDS             6449..7102
                     /locus_tag="VEL68_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HXZ36576.1"
                     /translation="MIDLHTHTLFSDGELLPSELVRRAEVIGYQAVALTDHADSSNLD
                     WMIPRIVQACQELNAHWKIRAIPGIELTHLPPGIIGRMVEKARVLGARVVVVHGESVV
                     EPVPPGTNRKALEAGVDILAHPGLISEGEVVLAREKKVFLEISARKGHSLANGHVARL
                     AQKCGAPMILNTDSHSPDDLITLEKARVVALGAGLEEEDFSRMLQNSRRIVEQLFLE"
     gene            complement(7137..8270)
                     /locus_tag="VEL68_11210"
     CDS             complement(7137..8270)
                     /locus_tag="VEL68_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXZ36577.1"
                     /translation="MSTNLRELLEEMVRQDASDMYITVDSPPMYRVEGVTFPWGEQKY
                     SPEETETMALTLMSETQKRYFTRRWEMNLSISYPNLGRFRVNILRQRGYFGLVFRSIK
                     IHIRTIDDLGLPQTFKELSMVKRGLVLVAGRTGSGKSTTLAALIDYRNANASGHIVTV
                     EDPIEFVHNHKMSIVSQREVGIDTHGYASALKHTLRQAPDVILIGEIRDLETMEAAID
                     YAETGHLCLSTIHANNANQAIERIMNFFPPERHRQIYLQLSLNLKAIISQRLVPSLDG
                     TRIAAVELLLDTPRVKDLIHKGQVDLLKEAMAQGAGEGMQTFDQSLFDLYHQGIIDLD
                     TSIGYADSANDVRLRIKMEEVGERKEGQELNLRLMPETHARAR"
     gene            complement(8282..9325)
                     /locus_tag="VEL68_11215"
     CDS             complement(8282..9325)
                     /locus_tag="VEL68_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXZ36578.1"
                     /translation="MDISELLLFVHKEGASDLHISAGEQPMARIHGEMRRIEVPSLSK
                     EDVHVMLYDVLNDQQRKKFEEQHELDFSIEMKGIARFRVNAFRQARGESIVFRVIPSK
                     ILSLEELGLPKILREICKQERGLILVTGPTGSGKSTTLASMIDVINNSLKGHIITIED
                     PIEFVHESKNSLINQRELGAHTHSFANALRSALREDPDIILVGEMRDLETISLALTAA
                     ETGHLVFSTLHTSGAPKTVDRVIDVFPGEQQAQIRAQFAESINAIVSQVLLKRRDGRG
                     LVPALEIMIGTPAVRNLIRESKVAQLPSAIQTGQKFGMVNLDQMLKDLVNRNIVEKEQ
                     CLYLASSPTIFNS"
     gene            9614..10513
                     /gene="xerD"
                     /locus_tag="VEL68_11220"
     CDS             9614..10513
                     /gene="xerD"
                     /locus_tag="VEL68_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXZ36579.1"
                     /translation="MSDDMDRWIDRFLHHLAVEKGLSPNTLEAYSRDLQGYSCLLRDK
                     GIREMRKISSEEPLTYMKRLRSRGVSARSQARVLSSLKGFHKFLLREQAVEEDPLRRL
                     RSPRVVPRLPSVMSTKEVEELLRQPDTRQPLGIRDRAMLELLYATGLRVSELVHLSVN
                     DLNLEVGYLRTRGKGSKERIVPLGKAAVHALKDYLTGPRRLYSSGTVNSTLFIRRGGK
                     GMTRQGFWKIIKNYARAAGMQKRITPHMLRHSFATHLLEGGADLRSVQSMLGHEDIAT
                     TQVYTHVSREHLKRLHQKLHPRG"
     gene            10517..13231
                     /locus_tag="VEL68_11225"
     CDS             10517..13231
                     /locus_tag="VEL68_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXZ36580.1"
                     /translation="MDVITTHINADFDSLASMLAAKKLYPEAVLVFPGSTEKNLRDFF
                     IQSTFYVFQTERIKNIDLKAVKRLILVDTRQAGRIGKFAEVVKRQGVELHIFDHHPPA
                     PGDLQGTVEQVREVGATVTILAQILQEKNMPLTPEEATILAVGLYEDTGSFTFSSTTP
                     EDLRAAAFLLSRGANLNTVSSLITRELTSDQISLLNELVQSAARHLVNGIEIVVAKAS
                     SDKYVGDFALLVHKLKDMENINVLFALAQMEDRVYLVARSRIEEINVGEIAAAFGGGG
                     HATAAAATLRDMDLAQAEGKLLDYLGKRIHPIRLARELMSFPVKTVDTDETIDHAGEL
                     LTRYNINVLPVMEDGHVAGLITRQVIEKAAFHGFKDSPVKDYMTTEFAVVNPQTPLTK
                     IQDLVVGNNQRFLPVLEKGKLVGAITRTDLLRWVYSRSDQTSPSVAEPDFFSAEPRKR
                     AIWKIMEERLPPGVLDLLKNIGLKAHELGLQAYAVGGFVRDLLLRNENFDIDVVVEGD
                     GIRLARALAEEGMGELRVHRRFGTATLRLSNRYRLDLATARLEYYEHPAALPRVEHSS
                     IKLDLYRRDFTVNALAVHLHPDHFGELIDFFGGQKDLKERVIRVLHNLSFVEDPTRLF
                     RALRFEQRIGFQIAKQTHMLMENAVRMKLFGRLSGRRLFRELVLCLSEEKPLPVLKRL
                     RDFGLLDLFCPGLKIDRSFETRFQRLEGVMFWYRLLFTGEKLEPWVTAFLGLVDGMAE
                     KELEDLLVRFSLAEKNRNSFLQRRRMAIQVVHRLFSSPPPGRSEVFFLLNPLPVDFIL
                     YAMGRSEEEGVKKSISMYFTELKRVKVSLRGKDLKKMGFVPGPIYAEILQAVLRARLE
                     GKISSRQEEIDFVLKRYNPRPPAPAEKRAHRHPHRKGKEGLRERTSAG"
     gene            13331..14314
                     /gene="trpS"
                     /locus_tag="VEL68_11230"
     CDS             13331..14314
                     /gene="trpS"
                     /locus_tag="VEL68_11230"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXZ36581.1"
                     /translation="MSKQRVLSGMRPTGKLHLGHLVGALDNWRQMQEEYDCFFFAADW
                     HALTTEYAETDIIRDSIREMMIDWLSVGISPEKSVLFIQSRIKEHAELHLLLSMITPV
                     PWLERVPTYKEQQQELTNRDLSTYGFLGYPLLQTADIIIYKAHKVPVGIDQVPHVELS
                     REVARRFNFFYGPVFPVPDPLLTEVPKLLGIDGRKMSKSYDNAIFISDPPEEVRRKTS
                     AMFTDPNRIRKSDPGNPDICNVFSFHKVYSDPETVIRVNRECRQATLGCVEDKKNLAE
                     TLVCYLAPLQEVRERFLRNPRLVEEILEDGCQRARSVAARTMEEVRAAMKI"
     gene            14448..15257
                     /locus_tag="VEL68_11235"
     CDS             14448..15257
                     /locus_tag="VEL68_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXZ36582.1"
                     /translation="MESSIFHTEPEQLELPLKVRLTAFEGPLDLLLYLIKKNEIDIYD
                     IPIAVITQQYLEYLEMMKNLNLDVAGDFLLMAATLLHIKSKMLLPPSAEEEGEGEKEE
                     EDPRAELVRRLLEYQRFKEAARELSKGPLLDREVFLRSFFGEDLAEGSREEEPGAEAS
                     LFDLLEAMKKVLEGLPPEDFQEISVESLNIKDKILQIMERLWENESVAFNELYTSSTS
                     RREIIITFLALLELLRLRMIRAFQGETFGTIRVFSPVAREEGRKTIEERIL"
     gene            15259..15924
                     /gene="scpB"
                     /locus_tag="VEL68_11240"
     CDS             15259..15924
                     /gene="scpB"
                     /locus_tag="VEL68_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXZ36583.1"
                     /translation="MEDAQLKAILEALIFVSGSPLSLERMKEIVGAVNKKDLQRLLDE
                     MVEEYRKGERGFSLVEVAEGFQFRTRTEHAEWVRKLMKIKTSTLSQPALETLAIIAYR
                     QPVVRGDIEKVRGVDSGGVLRTLLEKKLVKIIGKKDVPGKPLVYGTSKRFLEVFGLKD
                     LSQLPTLKDLDSLGPASMEGVLPLEEEEEKRKAPEAIEPQAQESQEAKIDESAPEAAE
                     GPC"
     gene            15887..16621
                     /locus_tag="VEL68_11245"
     CDS             15887..16621
                     /locus_tag="VEL68_11245"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXZ36584.1"
                     /translation="MNPLPRLQKVLAEMGLASRRRAEDMIREGRVTVNGRAARIGDKV
                     DPSHDHIKVDGRRVALPSSKVYLLLHKPKNTVTTVEDPEGRPTVLGLVKERGPRLFPV
                     GRLDYDAEGFLLLTNDGDLAHRLSHPSFRIPRAYWVKVKGKPSAEEIRKLSRGISLED
                     GPTAPCRITTLRETQENAWMEMTLREGRNRQVKRMWEKMGYPVLKLKRISFAGLTLGD
                     LQPGEYRDLRPKEVEKIKRMIKDPSA"
     gene            16661..17197
                     /locus_tag="VEL68_11250"
     CDS             16661..17197
                     /locus_tag="VEL68_11250"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXZ36585.1"
                     /translation="MAIKFSKKGDRGFTSLMGGQRVSKSGPRPEAYGTLDEASSVLGM
                     ARAWAKKTRTREIILSIQKDLLILGAELATESQDSLKFNYQVHAQNVDRLEQLMEELQ
                     NQVTLKNEFVYPGDSPAGAVIDLARTIIRRGERRAVGLLRQKKVQNPEVLRYLNRLAD
                     LLFVLARYEDDSSLPCTP"
     gene            17185..18567
                     /gene="tilS"
                     /locus_tag="VEL68_11255"
     CDS             17185..18567
                     /gene="tilS"
                     /locus_tag="VEL68_11255"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXZ36586.1"
                     /translation="MHPLILAVRKTLQKYDMVQPGDRVVVAVSGGADSVALLHSLWAI
                     RLDFHFSVVVAHLDHGLRPEGEKEALWVGKMAGKLGIPFHRGEVDVRSFQKEKALTLQ
                     EAARELRYSFLQGVARKCQAAKIALGHTAEDQAESLIIRLLRGSGTRGLSGIPPMRDG
                     IYIRPLIETWRKEIEDYLKDQKIAYLTDPSNQCLQYLRNRVRHELLPLLQQYNPRIRQ
                     ILVQMADLFRAEEEFWKKHLEEKFPAVVRSRKKDALSLDIPCLVSQPLPLRFRCLRRA
                     VETVQGHLRRVSLSHIWAIQGLLEGTEPNKILRLPHGLTVTRAYNVLNLTRSQEEAAP
                     FEYSVPGPGYVEIPEIGRSMRFEVQGRKRKVFFEDSPNVALLDFDALDFPLTIRTFRP
                     GDRFQPLGMEGEKKVKDLFMDCKIPTLYRKRVPILFQGERILWVTGIRIDHRARLKPE
                     TQKILRAELI"
     gene            18758..>19512
                     /locus_tag="VEL68_11260"
     CDS             18758..>19512
                     /locus_tag="VEL68_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HXZ36587.1"
                     /translation="MSPFYKNLALWLVISLMMILLFNLFNQPRPSQDKVIFSDFLTAL
                     ERGEVREVTIQGHNIYGRYNNLKEFRTFTPSYPDLIKALKEKNVKISAKPEDDSPWYM
                     TMLISWFPMLLLIAFWIFFMRQMQAGGTKAMSFGKAKVKLLSDKQQKVTFQDVAGIEE
                     SKEELQEIIEFLQDPKKFTRLGGRIPKGVLLVGPPGTGKTLLARAIAGEANVPFFSIS
                     GSDFVEMFVGVGASRVRDLFIQGKKHAPCIIFI"
BASE COUNT         4627 a         5076 c         5575 g         4234 t
ORIGIN      
        1 ttgttagggt acgaatttac ccgctggatt tcaacctttt tgactcgcct gctcttattt
       61 tccttgattc cgctttgaat ccttgcctat actcattttg gacaaacgtg gtcgttcctc
      121 gtttgttata atgaaaacca cgcttacttc atgcggccag cgagagatgt atgaccgaat
      181 gccgaggaaa atgaacgacc cccaggccgc gatccatgcg cctgctgagg gataaaaaga
      241 aaggagagac tcatgcgttt tccggatttc attgaaaagc tccccaaggc tgatcttccc
      301 gttccggaga tcgaggggtt cttgttacag gcggaaaaac atcaggtcgc ttttttgcgg
      361 tcggaaaagg atatgctgat gcccgaacac tcccatgctg cccagtggga aatccctctg
      421 gaagggagtg cggagctcac catggacggg caggttaagg tcatgagacc ggggcagccc
      481 atctacattc ctgccggggt tccccattct ggaaaggtca aggggcctta tgctgcgatc
      541 attgtctttg acgcgccagc caggtacaag gtgaaaaaat gaaaagcatg gcgcttagag
      601 catagggcat agcgtcaaaa atggagcaca aaatttagcg catggggaag agcggctagc
      661 gaaaagcggg cgagggagag gaggacccgg tccttgttgg gacggatttg aggatgattc
      721 tctatctccc tactggttcg cgggttcttt cttttcatat ttcaagcact tcattccgga
      781 agcctggaaa accatatgcg agggcatctc cctggattta aaacctaggg catgacaacc
      841 gcgggggaag ttcttatccc aggtgacgaa gaagtgctgg catttaaagc agtcgacctt
      901 ctcgggtttc atggatttag aaacggcata gcgcagagag catagggcat agaacaaagc
      961 aagaaaatcg gcagaaaaac taggcgatga ttttgaaaag aaagattgca gtcatgatca
     1021 cggcgatgat cagctttccg atggttccgg ctaaaaaacc caccaaggtc ccaatcccac
     1081 tcttgaggga tgcctgaagg tctttctgtt tgaccagctc aaaaagaaag gctccaacaa
     1141 aaggaccgat gagcagcccg aagaggcctc cgataaccgt gccgacgatc atgccgagca
     1201 aagccccggc cattccccag ccgctacccc ccattttttt aacccccagc atggaggcaa
     1261 aaaattcaag gaagtagctc aagagcatta aaaccaacag caccgcaagg acaccccagg
     1321 tgacggttgc aaaccccgta taccatccat agaccagggc tccggccaga atcaaggggg
     1381 ctcccggaag ccctggcagg acagacccga ggagcccgat aagcatgagg atgaaagtga
     1441 cgatgagaat caggatatgt tcagccatgg ggtaatccta ctcgaacccc ttcttttgtc
     1501 tgaagtccac aaagacattt gcaaaaaaaa accttttccg gctgcaaagc tgaattccat
     1561 tttactattt tcccccggag attgaaattg ataagtatga tcagtcaaga atatgcggga
     1621 attgggggga tcattatcaa agggcatgag atctttacgc cccggggctt gaagtagcac
     1681 cacccaaggg gaccctctta ttggcgactc ggaggtctca aagattggaa ttgacaagga
     1741 agatcagaat ccggctaata tggaaggagc gactcattct ccttttgaag acgcaggatg
     1801 ggtcgaatct tgcccggggg gaaaagaatg gaaaaaggta atttaagagt caatgcgaaa
     1861 cgtcttcggg cgaccatcga agaattggcc cgaatcgggg gtactgccgg aggaggtatg
     1921 caccgtctgg cgttaaccga tgaggataaa caggccaggg atttattcgt ccaatggctg
     1981 aaggaactcg gcctggaaat cacggttgac gaaatgggga atattttcgg aaaacggaag
     2041 ggaaggagcc cccagttggc catggtcctg agtggctccc atctggatac tcaacccaag
     2101 ggcggacgat ttgatggaat cctgggggtc acaggggcgc tggaagcgct gcggacgatc
     2161 catgaaaacc gcatcgatac ggaacggagc atcgccatcg tagattggac agacgaggag
     2221 ggtacccgtt tcgcccctgc gatgctgggc tccggggtct gggcgggcca tttcgatcgc
     2281 gactgggctt acgcccgggc ggaccttgag ggaaaaagct tcggagaggc attggaaagg
     2341 atcgggtaca agggcaaagc gccggcgcgg aaatggcctg tccatgccta ctatgaattt
     2401 catattgaac aagggccggt tctggaaaag atgggtaaac gaatcggatt gcccaaagga
     2461 attctcggaa ttacctggtt cgatgtttat gtagagggac aggccaatca agcaggcccc
     2521 acgcctatga attcccgcaa ggatgccctg gttgcggcag cggagatgat tcttacggta
     2581 agcgaccttc ccgcaaagat ggggggagac ttggtaggca ccgtgggcga gattcagaat
     2641 tttcccaact cgaggaatat tattcccgac cgggtgcggt ttaccgtgga tattcgctcc
     2701 tgggatgacc ctttcaagaa ccaagcggcc gaggcaatca agaaagagtt tttccagatt
     2761 ggccagaagc ggggatgttc agttagaatg gaaatgatct gggacatcct ccataccgag
     2821 ttcgcctcaa atctggtggg aagggtagag aaagtcgcct ctctgcttgg gtattcaaac
     2881 tattccatgg tgagcggagc ggggcacgac gcggcctatg tctgccgggt ggctcccaca
     2941 gccatgatct tcgtccccag catcggcggc cgcagccacg tggaagttga aagaaccggc
     3001 tgggaggact gcgaagcagg ggctaacgtg ctgctccatt gccttcttca atcagccaac
     3061 gagtgagcca acccatggcc gaaggcatat ggtttcttcc cgggaatatt ttcaggagag
     3121 cctggagaaa attaccttgg ggtgggggag aaatggaact tatggagcaa ggtaaattta
     3181 aagagtcgag tcgatgaaat ggcctttctt ttgaacccgg atcatccttc cttgccgatt
     3241 atagacggat gaatcgtgac taccttctct tgtcgacgtt tggtcgtaag gttcgacgga
     3301 cacctcaatt cccggagcca ctgcaaaata gttctccacc ggcgatggat ggtagagaac
     3361 taacccttct ccattggtga atggaaagtg agcagcaagg gcatgaacca tttttcacgc
     3421 ctcagaatcg cttacgattc ttcttcctgg gccgcgtgtt taactggagt ttcgcctcaa
     3481 gaccggaagg atgaattttt ttaacttatt aggatttaaa caaaaagtca acaaaaatat
     3541 gcagaatatg gagggatatg gagatttggg tagcgggaga tttggaaaag ctgaataaat
     3601 tcaattggat agagaataga ttgggatata gatgaaagaa aaggtctgac gtctaaattc
     3661 ggtttgaagc ccgaagatta atcaagatgc tcccggaaaa attcgtcttt ccgatgaaga
     3721 cccaagtcag gggaacttaa cgctcattga aggagcagga ttccggctcc ccgctttaac
     3781 cctgaccgga gaggtttcaa cggaaggaag caaaagcctg tcattgattg attgcaggtc
     3841 ttctttggct gaatggtgaa atatcaagaa gaaagggaat catccgtctg aggatttctc
     3901 cccataacaa agtgaaacat ggattgggct tttcccatct cctcctgcag aatctgttcc
     3961 acagaagcca agtcggaaga gtcccacgtg gaagggtagt tcatcccttc cctgctgaaa
     4021 gactccagca tgttgaaaaa attcatctcc ccgaaggtga atttgtcggc gaaatggaga
     4081 attttcccca aggtcgggtt aaccgtccaa gagcaatcat gatgatggcc gatgacatcg
     4141 atcaaattgg gggaaaatgc ccagcgttca gcaaccttgc ccccgatttc agcatgggtc
     4201 gtctccagga cctctttttc ggcttgcagc cattccatgc ccgatttttt tctctcctcg
     4261 atttcgttga attgttccga aagaaattgc tggatcacta ggcgcccgat atcatgggtc
     4321 aacccggata gataagcctc atccaaggaa tcataccggt aaatccaggc taggcgtttg
     4381 gagatcaagg cgcattgaag agagtgctgc cagagaggct ggagggactg aacatcccga
     4441 acgtcgtgca aggtgtggaa aaaggaggta gtcaggatga tttccttgac cgattcaaac
     4501 ccgatcatca tgatggcgtg gtggatgctg ttgattttcc ggccttttcc cccataataa
     4561 gcggaattag ccactctcaa aactttagct gccaaagact ggtccaggac cctagaaagc
     4621 tcgacggcgc ttatcttggg cgattcagcc atggacaaga ctctcataac cacctggggt
     4681 agggtgggaa gatgctcaat ctgttggatg acttgatcga tgatagcccg ttttttcccc
     4741 gtggaaaggg gtttctggtt aaaaaccatt tcctttgggt ttgaaggctg ctctaatctc
     4801 atagtcttac cccaagacct cctacccata tacttcggca gtttgaccaa aaaatttaag
     4861 tacttataag gagaaaactt gggggaaaag cttagaaagg aaggccaaga gactacccag
     4921 ccaggggatg gatccaccga attgacaggg actgacggag gggggataat aaataaattc
     4981 tatgaatccc aggaaatttt tcatccagac cttcggatgt cagatgaatg tttatgattc
     5041 cgagcggatc gcccaggagc ttaccgctcg ggattaccga caggtgaatg atcctgcaag
     5101 ggcggacctc attttggtaa atacctgcag cgtgagagaa aaacctgagc aaaaagtcta
     5161 cagcgctctg ggccgatttc gaaggcttaa aaagaagaac ccgaagctca tcatcggggt
     5221 gggaggatgc gtagcccaac aggaggggca gggactcttg gagagggtcc ctttcctgga
     5281 tttcgtactg gggaccaagg agctgtgcag gattcacgag atcctggacg atctggaaaa
     5341 atcgggagaa cgggggtgcg ccgtcaatct ggacggaaga atcgacccct atcggggact
     5401 ggcgctttct cccgatcccg ggagaccaac ctcttttatc tcgatcatgc agggatgcga
     5461 caacttctgc tccttttgcg ttgttccctt tgtgcgtggc cgggaagtta gccggccaag
     5521 caaggacctc atgaaagaga ttggagttct ttcgtcccaa ggagtaaaag aggtgaccct
     5581 gctggggcaa aacgtgaact cctatgggaa gcagaccccc ggggaaccgg gtttcgtgga
     5641 gcttttggaa agggttgaaa agatcccggg gatcgagagg gtccgattca ccacgtccca
     5701 tcccaaagac ctttcttcgg aactgatcca agccttcggc cgattgacca agctttgcga
     5761 acacattcat ctcccgctcc agtcggggtc aaaccgcatt ctgaagcgaa tgaaccgggg
     5821 gtacacaagg gaggaatatg aggaaaaggt tctgaacctg cgccagacct gcccggagat
     5881 cagcattacc agcgatatca tcgtgggctt tccgggagaa gaagaaagag accaccagga
     5941 aaccctggag atgatcagaa aaattcaatt tgacgatctg ttttccttcc gctactccga
     6001 tcgcccccat acccgggcct cactcttccc ggacaaaatt gcggaggaat tgaaacaacg
     6061 gcgtctttcc gaactccagg cggcgcagaa ggacatcact cagagaaaga accaagcctg
     6121 ggagggtaga gatgtggagg tcttaactga agggcccagc aaggccaagg cagaagagag
     6181 gaccgggagg acccgtacca accggatcgt gaattttccg ggagattcgg ttccgattgg
     6241 ggctttggtg agggtgcggg tctggcatgc cctggcccat agtctaaggg gggagcttct
     6301 ataagagttt caagcggcac acttcaggga cagccaagag agccggataa gatgaggaaa
     6361 gagcagtggg gactttgaaa gaagaggtct aaaaatttgg ataaagaaag cgataactcg
     6421 tgtcactgtg catccggagg cttccctcat gattgatctt cacacccata ccctgttcag
     6481 cgatggggag cttctgccat cggaactggt tcgccgggcg gaagtaatcg ggtaccaggc
     6541 ggtggccctc accgaccacg ccgattcttc caacctggac tggatgattc ccaggatcgt
     6601 tcaggcgtgt caggaattaa atgctcattg gaaaataagg gccatccccg gaattgagct
     6661 tacccatctc cccccgggga tcattggtcg catggtggag aaagcccggg tcttgggggc
     6721 cagggtagtc gtagtccacg gggaatccgt ggttgaacct gtgccgcccg gaaccaaccg
     6781 gaaggccctg gaggccggcg tggacatcct ggcccatccc ggactaatct ctgaagggga
     6841 agtggtcctg gcccgggaga agaaggtttt tctggagatt tcggctcgca aaggacacag
     6901 cctggccaat ggacatgttg cccgtctggc tcaaaaatgc ggggcgccca tgattttgaa
     6961 cacagattcc cactcgccgg acgacctgat cactctggaa aaagcacggg tggtggctct
     7021 cggcgccggg ctggaagagg aagacttttc aaggatgctg cagaattccc gccggatcgt
     7081 ggaacaacta ttcctggaat gaagaataac ccttcccgtt gtcctgaacg tgaatcctac
     7141 cgggcgcggg catgggtttc gggcatgagt cgcaggttca gttcttgccc ttccttcctt
     7201 tcacccactt cttccatttt tatcctgagg cgcacgtcat tggcgctgtc cgcatagcca
     7261 atgctcgtgt ccaaatcaat gatcccctgg tggtagaggt cgaagaggga ttggtcgaag
     7321 gtctgcattc cttcccccgc gccctgagcc atggcttcct tcaggaggtc gacctgtcct
     7381 ttgtgaatca ggtctttgac cctgggcgta tccagaagga gttccacggc tgcgattcgg
     7441 gttccgtcca agctggggac caggcgctgg gagatgatgg ccttgagatt taaggaaagc
     7501 tggaggtata tctgccggtg cctctcggga gggaagaagt tcatgatccg ttcgatggcc
     7561 tggttggcat tgttggcgtg tatggtggac agacagaggt gcccggtttc agcgtagtcg
     7621 atggccgctt ccatagtttc caggtcccgg atttccccga tgagaatcac atcgggggcc
     7681 tggcgcaggg tgtgtttcag ggcgctggca tacccgtggg tgtcgattcc cacctctcgc
     7741 tgactgacga tggacatttt gtgattgtgg acgaattcaa tggggtcctc gacggtcacg
     7801 atgtgcccgc tggcgttggc gttccggtaa tcgatgagcg ccgccagggt ggtcgatttg
     7861 cctgaccctg tgcgcccggc cacgagaacg agcccgcgct tgaccatgga cagctctttg
     7921 aaggtctggg gcaaccctaa gtcgtcgatg gtacggatat ggattttgat ggaccggaag
     7981 accagcccga aatatcctct ctggcgcaag atgttgaccc gaaaccggcc taagttggga
     8041 taagatatgg agaggttcat ctcccagcgg cgggtgaagt accgtttctg ggtttcactc
     8101 atgagcgtca aggccatggt ttccgtctct tcgggggaat acttctgctc tccccagggg
     8161 aaggtgactc cctcaacccg gtacatgggc ggactatcga cggtgatata catgtcggaa
     8221 gcgtcctgcc ggaccatctc ttccagaagc tcgcgcagat tggtgctcac gataagtctc
     8281 ctcaggagtt gaaaatggtt ggggaactag ccaggtagag gcactgttct ttttccacaa
     8341 tattccggtt caccaggtcc tttaacatct gatcgaggtt gaccatcccg aatttctggc
     8401 cggtctgaat ggccgaggga agttgggcca ccttgctctc tcggatcagg ttgcggaccg
     8461 ccggggtccc gatcatgatc tccagggccg gcaccaagcc acggccgtcc cgccttttca
     8521 acaggacctg ggagacgatg gcgttgatgg actcggcgaa ctgagcacga atctgggcct
     8581 gctgttcccc gggaaagacg tcgataaccc ggtccacagt tttgggggct ccgctggtgt
     8641 gcagagttga gaagaccaga tgtccggttt ccgccgcagt cagagccagg gaaatcgttt
     8701 ccaaatcccg catttcaccc accaggatga tgtccgggtc ctcccgcagg gcgctgcgca
     8761 gggcgttggc aaaactgtgg gtgtgcgctc ccagttcgcg ctggttaatc agggaattct
     8821 tggattcgtg gacgaactcg atggggtctt caatggtgat aatgtggcct ttcagggagt
     8881 tgttgatcac atcgatcatg gaggccaggg tcgtggattt tccacttccc gtaggaccgg
     8941 tgaccaggat cagcccccgt tcctgcttgc agatctcccg gaggattttg ggcaggccca
     9001 gctcctccag agacaaaatc ttggaaggaa tgacccggaa gacgatcgat tctccccggg
     9061 cctggcggaa ggcattgacc cggaatcggg cgatgccttt catctcgatg gaaaaatcca
     9121 gctcgtgttg ttcctcgaac ttcttcctct gctgatcgtt caggacgtca tagagcatga
     9181 cgtggacgtc ttccttgctg agggagggga cctcgatacg gcgcatctcc ccgtggatgc
     9241 gggccatggg ctgctctccc gcgctgatat ggaggtcgga ggccccttcc ttatgcacaa
     9301 agagtaagag ttctgaaatg tccattgttt taactcctta tcggctggga acccacaaat
     9361 atttaattaa aaaaagccaa ctctgtcaag ctctttgctc aatttggaag gaagaaccat
     9421 ccccaacttt taccatcgga agatgctggg gaaacctgaa agattggacc cctgggattg
     9481 cggtatgata aaaatttcga ttgctcccaa gcatatgccc catttgcttt cccatttaca
     9541 ggacggggga gaaagaaatt gaaaaaagaa accaatgtgt tagaatgaga accgaattgg
     9601 gggccgatgc cgcatgagcg atgacatgga ccgctggatt gatcgttttt tgcaccatct
     9661 ggctgtggaa aaagggctta gccctaatac cctggaagcc tattcccggg accttcaggg
     9721 atattcatgc ttgcttcggg acaagggaat tcgggagatg agaaaaatct cctctgaaga
     9781 accattgacc tacatgaaga gactccgctc ccgcggggtg tcggctcgct cccaggcccg
     9841 ggtcctctcc tcgctgaaag ggtttcataa gtttcttctc cgggagcagg cagtggagga
     9901 ggaccccctg cggaggctcc gatccccccg ggttgtcccc cgccttccct cggtcatgtc
     9961 cacgaaggag gtggaggaac tgctgcggca acccgacacc cgtcagcctc tcggaatccg
    10021 cgaccgggcc atgctggaac tcctgtatgc gacaggcctt cgggtttcgg agctggtcca
    10081 cctttcggtc aatgatctga acttggaggt gggttacctg cgtacccggg gaaaggggtc
    10141 caaggagaga atcgttcctt tgggaaaagc cgccgttcat gccttgaagg actatttgac
    10201 cgggccccgc cgcctctact cctccggcac ggtcaactcg accctgttca tcaggagggg
    10261 agggaagggg atgacccgtc agggattctg gaaaatcata aagaattacg cccgggcagc
    10321 agggatgcaa aagaggatca ccccccatat gctccgccac tctttcgcca cgcacctcct
    10381 ggagggtggg gcggacctga ggtcggtgca gtccatgctc gggcatgagg atattgctac
    10441 cacccaggtt tacacccacg tctcccggga acacctgaaa cggctgcacc agaagttgca
    10501 tccccggggt tgatccatgg acgtcattac cacccacatc aatgccgatt ttgattctct
    10561 ggcatccatg ctggcggcca agaagctcta cccggaggct gtgctggtct ttcccgggtc
    10621 cacggagaag aacctgcgcg acttcttcat ccagtcgacc ttctacgttt ttcagacgga
    10681 acggataaag aacatcgacc tgaaggcggt caagcgattg atcctggtgg acacccgaca
    10741 ggccgggcgg atcggcaaat tcgccgaagt ggtaaagcgg caaggggttg aactgcatat
    10801 ctttgaccat caccccccgg cccccggaga cctccagggc accgtggaac aggtgcggga
    10861 agtgggagcc acggtgacca tcctggccca gatcctgcag gaaaaaaaca tgcccttgac
    10921 cccggaagag gccactatct tggcggtggg cctctatgag gacaccggct ctttcacttt
    10981 ttcctccacc acccccgaag acctccgggc ggcggccttc ctgttatccc ggggggctaa
    11041 tttgaacacc gtctccagcc tgatcacccg ggagctgacc tcggatcaga tttctctcct
    11101 gaacgaactg gtccagagtg ctgcccgcca cctggtgaat gggatcgaaa tcgtggtggc
    11161 caaagcctcg tcggacaagt acgtgggaga tttcgccctt ctggtccaca agctgaagga
    11221 catggagaat atcaacgtcc tcttcgccct ggcccaaatg gaagaccggg tctatcttgt
    11281 ggcccgcagc cgaatcgagg agatcaatgt gggcgagatc gctgcggcct tcggcggagg
    11341 aggacacgcc acggccgcgg ctgccaccct tcgcgacatg gacctcgccc aggcagaagg
    11401 gaagctcctc gactatcttg gaaagcgcat tcatcccatc cggctggccc gggaactcat
    11461 gtcctttccg gtaaaaacgg tcgacaccga cgagaccatc gatcatgcgg gggaactcct
    11521 cacccggtac aacatcaacg tcttgccggt gatggaagac gggcatgtgg ccgggctgat
    11581 cacccggcag gtgatcgaaa aggcggcttt tcacggtttc aaggattctc cggtgaaaga
    11641 ctatatgacc acagaattcg ctgtggtcaa cccccaaact ccgctgacca aaattcaaga
    11701 cctggtggtg ggaaacaacc agcgattcct acccgtttta gaaaaaggca agctggtggg
    11761 ggccattacc cggaccgacc ttttgcgctg ggtctacagc cggagcgacc agacgtctcc
    11821 ctccgtggca gaaccggact ttttttccgc tgaacctcgt aagcgggcca tctggaagat
    11881 catggaagag aggctccctc cgggggtgct ggaccttttg aagaacatcg ggctcaaggc
    11941 ccatgaactg ggcctacagg cctacgccgt gggagggttc gtccgggacc ttctcctgcg
    12001 gaacgaaaat ttcgacatcg acgtggttgt ggaaggggac ggaatccggc tggcccgggc
    12061 cctggccgag gaaggaatgg gggagttgcg ggtccaccga aggttcggca cggccacgct
    12121 ccgcttgtcc aaccgttacc ggctggattt ggccacggcc cgcctggaat attacgaaca
    12181 ccctgccgct ttgccccggg tggaacatag ctccatcaag ctggaccttt accgaaggga
    12241 ttttacggtc aacgccctgg ccgttcacct gcatcccgac cacttcggcg agctcatcga
    12301 ttttttcggg gggcagaagg acctgaaaga gcgggtgatc cgggttctgc ataacctgag
    12361 cttcgtggaa gaccccacgc gactcttccg ggccttgcgc ttcgaacagc gtatcggatt
    12421 ccagatcgcc aagcagaccc acatgctcat ggagaatgcg gtgcgcatga agctcttcgg
    12481 ccggctttcg ggaaggcggc tcttccggga gctggtactc tgcctgtcgg aagagaaacc
    12541 cctgccggtg ctcaagcgcc tgagggactt cggtctgctg gacctcttct gccctggttt
    12601 gaagatcgat cgttctttcg aaacccgctt ccagcgcctg gaaggggtca tgttctggta
    12661 ccggctgctc tttaccgggg aaaagctcga accctgggtc acggccttcc tggggctcgt
    12721 ggacggcatg gccgaaaaag aactggagga cctcctggtc cgtttttccc tggcggaaaa
    12781 aaatcggaac tctttcctcc agcggaggcg tatggccatt caagtggtcc accgtctctt
    12841 ttcctcgccc cccccgggga ggagcgaagt cttctttctc ctcaatcctc tacccgtgga
    12901 ttttatcctc tacgccatgg gaaggagcga ggaagaaggg gtcaagaaat cgatctccat
    12961 gtattttacc gaactgaaga gggtcaaggt ctcgctcagg ggcaaggacc tgaagaagat
    13021 gggctttgtt cccggcccta tttatgcgga aattcttcag gccgtgctgc gggcgcgact
    13081 ggaaggaaaa atttccagcc ggcaggagga gatcgacttt gttctgaaga gatacaaccc
    13141 tcgccctccg gcccccgccg aaaagagggc ccaccgccat ccccaccgga aggggaaaga
    13201 gggactgagg gaaaggacct ccgcgggtta ggcccgtaat tcgccttcgc agggaaggcc
    13261 tgggcgggcg aggagagcag acaggatagg gaaggtttta aattcgtcag aagggggaag
    13321 gttatttttc atgtccaagc agagagtatt gagtggaatg cggcccaccg gcaaacttca
    13381 tctgggacat ctggtggggg ccctggataa ctggaggcag atgcaggaag agtacgattg
    13441 ttttttcttc gccgccgact ggcacgcctt aactactgag tatgccgaga cagacatcat
    13501 ccgggattcc atccgggaga tgatgatcga ctggctgagc gtgggaattt ctccggagaa
    13561 gagcgttctt ttcatccagt cgcgaatcaa ggagcatgcc gagctccatc ttcttctgtc
    13621 catgattact ccggttccct ggctggaacg ggtccccact tacaaggaac agcagcagga
    13681 actgaccaac cgggaccttt ccacctacgg atttttggga tatccacttc tgcagaccgc
    13741 ggacatcatt atctataagg cccacaaggt cccggtgggg atcgatcagg tgccccacgt
    13801 ggaactttcc cgggaagtgg cccggcggtt caattttttc tacggaccgg tgttcccggt
    13861 acccgacccg ctcctgaccg aggtccccaa gctcctgggg atcgacgggc ggaagatgag
    13921 caagagctac gacaacgcca tcttcatctc cgacccgccg gaggaagttc ggcggaagac
    13981 cagcgccatg tttaccgacc cgaaccggat caggaagagc gatcccggca atccggacat
    14041 ctgcaatgtc ttttcgttcc ataaggtcta cagcgacccg gaaaccgtaa tccgggtcaa
    14101 ccgcgaatgc cgtcaggcga ccctgggatg cgtggaggac aaaaagaacc tggccgagac
    14161 cctggtctgc tatctggccc ccctccagga ggttcgggag cgttttcttc ggaaccccag
    14221 actggtggaa gaaatcctgg aagacggctg ccagcgggcc cggagtgtag ccgcccggac
    14281 catggaagaa gtgcgggctg cgatgaagat ttgaactcgt cgctggttgc tcgttacggg
    14341 tcgctggttt aagaaaagag ttcttagccg gcaacgaaaa ccagaaacca gtgaccagga
    14401 accagaatta attcattttt cttgaatacc gatcaaaaag gaaagggatg gaatcttcta
    14461 tttttcatac cgagccggaa cagctggagc tgccccttaa ggtcaggctg acggccttcg
    14521 aggggcctct ggacctcctc ttgtacctga tcaaaaagaa tgagatcgac atttacgaca
    14581 tccccatcgc ggtcatcacc cagcagtatc tggaatatct ggagatgatg aagaacttga
    14641 atctggacgt ggccggggat tttctcttga tggccgccac cctcctgcac ataaaatcca
    14701 aaatgctcct acccccttcg gcagaggaag aaggggaggg agagaaggaa gaagaggacc
    14761 cccgagcaga gctggtccgg cgactcctgg agtaccagcg cttcaaagag gcggcccggg
    14821 aattgagcaa aggccctttg ctggaccgag aggtcttcct ccgcagcttt ttcggggagg
    14881 acctcgcgga aggctctagg gaagaggaac cgggggccga ggcaagcctc ttcgacctgc
    14941 tggaggccat gaagaaagtc ctggagggac ttcccccgga ggattttcag gagatctcgg
    15001 tcgaatccct taacatcaaa gataagattc tccagatcat ggagcggctt tgggaaaacg
    15061 agagcgttgc tttcaatgag ctctacacct cctcaacctc acggagagag atcatcatca
    15121 cctttttggc cctcctggag cttttgcggc tgcggatgat cagagccttc cagggggaga
    15181 ctttcggcac gatccgggtc ttttctccag tggcccggga ggaaggacgg aagaccatcg
    15241 aggaaaggat cctgtgaagt ggaagacgcc caactgaaag ccatcctgga agctctgata
    15301 ttcgtctccg ggagcccctt gtccctggag cggatgaaag aaatcgtggg agccgtaaat
    15361 aagaaggacc tgcaacgact gctcgatgaa atggtggagg aataccgcaa gggcgagcgg
    15421 ggcttttccc ttgtggaagt ggctgaaggg tttcagttcc gcacccgcac cgagcatgcc
    15481 gaatgggtcc gcaagttgat gaagatcaag acctccactc tcagccaacc tgccctggag
    15541 accctggcca tcatcgccta ccggcagccc gtggtccgcg gggacattga aaaggtccgc
    15601 ggggtggatt cgggaggagt gctgcggacc cttctggaga agaagctggt caagatcatc
    15661 ggcaagaagg acgttccggg caagccgctg gtttacggca cctccaagag gttcctggag
    15721 gtcttcggtt taaaagacct ttcccagctt cccaccctga aggacctgga ttctctggga
    15781 cctgcttcga tggaaggagt tctgcctttg gaagaagaag aggagaagcg aaaagccccg
    15841 gaggcaatag aaccccaagc gcaggagagt caggaggcga aaatcgatga atccgctccc
    15901 gaggctgcag aaggtccttg ctgaaatggg attggcttcg aggaggcggg ccgaagatat
    15961 gatccgggaa ggccgggtta cggtgaacgg gcgggcagcc cggatcggtg acaaggtcga
    16021 tccttcccat gatcatatca aagtggacgg ccggagggtc gcccttcctt catcgaaggt
    16081 gtaccttctt ctccacaagc ctaaaaacac ggtcactacg gtagaagacc ctgaggggcg
    16141 gccgaccgtc ctgggtctgg taaaggaaag aggtccccgt ctttttcccg tgggccgctt
    16201 ggactacgat gccgaaggtt tcctccttct gactaacgac ggagacctgg cccatcggct
    16261 atcccatcct tccttccgaa ttccccgggc ctattgggtc aaagtgaagg gaaagccttc
    16321 agcggaagaa atccgcaagc tgagccgggg gatttctctg gaggatggtc ccaccgctcc
    16381 ctgccggatc acaactctca gagaaaccca ggagaacgct tggatggaga tgactctccg
    16441 ggagggacga aatcggcagg tgaagaggat gtgggagaag atgggatacc cggtcctgaa
    16501 attaaagaga atttctttcg cgggtcttac cctgggagac ctccaaccag gcgaataccg
    16561 cgacctgcgg cccaaagagg tagaaaagat caaaaggatg atcaaagacc cttcggccta
    16621 gggtggggct ggagaggcga gagtcggttt tgggggagct atggccatca aattttctaa
    16681 gaaaggcgat cggggcttca ccagcctcat gggcggccag agggtttcca aatccggtcc
    16741 gcggcccgaa gcctatggga ctctggatga agccagttcg gttttgggaa tggcccgcgc
    16801 ctgggccaag aaaaccagga cgagagagat tatcttaagc atacagaagg acctgctgat
    16861 tctgggggct gaattggcca ctgagtccca ggattccctt aagttcaatt atcaggtcca
    16921 cgcccaaaat gtggatcgcc tggaacaact gatggaagaa ttgcagaacc aagtgactct
    16981 gaaaaatgaa ttcgtctatc ccggagattc gccggccggc gcggttatcg atctggcacg
    17041 gaccatcatc cggcgcgggg aaagaagagc cgtgggtttg ctccgtcaaa aaaaggtcca
    17101 aaaccccgag gtcctgcgat acttgaatcg cctcgcagac ctcctctttg tcctggcccg
    17161 ctacgaggat gattcgagct tgccatgcac cccttgatcc tggccgtgcg caagaccctt
    17221 cagaaatacg acatggtcca acccggagac cgggtggttg tggctgtctc cggcggagct
    17281 gactcggtgg ccctgctcca ttccctttgg gcgatccggt tggatttcca tttctccgtg
    17341 gtggttgccc acctcgacca tggcctgcgt ccggaggggg agaaagaagc cctttgggta
    17401 ggcaagatgg ccgggaagct tggaattcca ttccatcgag gagaagtgga tgtccgttcc
    17461 ttccagaagg agaaggccct gaccctccag gaggcagcgc gggaattgcg ctattccttc
    17521 cttcaaggag tcgcccggaa atgccaggcc gctaaaatcg ctctgggtca tacggctgaa
    17581 gatcaggcag aatcacttat tatccggctg ctgagagggt cggggacccg cggcctgtcc
    17641 ggcattcctc cgatgcggga tggaatttac attcgacctc tgattgaaac ctggaggaag
    17701 gagatcgagg attacctcaa ggaccaaaag atcgcttacc tgaccgaccc atccaaccaa
    17761 tgtcttcagt accttcggaa ccgggtcagg catgaactcc ttcctcttct ccaacagtac
    17821 aacccgcgaa tccgtcaaat tttggttcaa atggccgatc ttttccgcgc cgaagaagaa
    17881 ttttggaaga aacacctgga ggaaaaattc cccgctgtcg tgcgaagtcg gaaaaaagat
    17941 gccttaagcc tggacatccc atgcctggtt tctcaaccac ttcccttgag gttccgctgc
    18001 cttcgtcgtg ccgtggaaac ggtgcagggc catctgcgga gagtcagcct atcccatatt
    18061 tgggctattc agggccttct ggaggggacc gaacctaata agattctgag actcccgcac
    18121 ggccttaccg taacccgggc atacaatgtc ctgaatctga cccgctctca agaggaggca
    18181 gccccctttg agtattccgt tcccgggccg gggtacgtgg aaattcccga gatcggccgc
    18241 tccatgcgtt tcgaggttca aggccggaag cgcaaggtct tcttcgaaga ttcccccaac
    18301 gtggctctac tggattttga cgccctcgac tttcccctca ctatccgtac cttccgaccc
    18361 ggagaccgtt tccagcccct tggaatggaa ggggaaaaga aggtgaagga cctctttatg
    18421 gactgcaaga tccccaccct ctacaggaag cgggttccca ttcttttcca aggcgaacgg
    18481 attctctggg taacaggaat ccggatcgat catcgggccc gattgaaacc ggagacccag
    18541 aagattctga gggctgaact gatttgagaa ggaagatgtt tcggagtccg ggatcgatgg
    18601 gtctttcggc tttaaaccac agactccacg ctctcacctg aaattcggtc tatctttttt
    18661 cgccggttta tgctataaaa agatatttgc ttgaaaccta agttttcaaa attcaattaa
    18721 aatctaactg ccgagcacaa ctatgggagg ggggaaattg agcccctttt ataagaattt
    18781 ggccttgtgg ctggtcatca gcctgatgat gatccttctc ttcaaccttt ttaaccagcc
    18841 ccgtccatcc caggataagg ttattttcag cgatttcctg accgctttgg aaagagggga
    18901 agtccgggag gtcaccatcc agggccacaa tatctacggc cgctataata acctcaagga
    18961 gtttaggacc tttacaccct cttacccgga cctgattaag gctctaaagg agaagaatgt
    19021 aaaaatctcg gctaagcccg aggatgattc tccttggtac atgaccatgc tcatctcctg
    19081 gttccccatg ctcctcctga tcgccttctg gatctttttc atgcgccaaa tgcaagctgg
    19141 ggggaccaag gccatgtcct tcggaaaggc caaggtgaaa ctcctctccg acaagcagca
    19201 gaaggtcacc tttcaggatg tggccgggat cgaagaatcc aaggaagagc tgcaggagat
    19261 catcgagttt ctccaggacc ccaaaaagtt tacccgtctg gggggacgga ttcccaaagg
    19321 ggtcctgctg gttggcccgc caggaacggg caaaaccctc ctggcgcgag ccatcgccgg
    19381 ggaagccaat gttccttttt tctctatcag tgggtcggac tttgtggaga tgttcgtggg
    19441 ggtgggggcc tcccgggtcc gggacctttt catccagggg aaaaagcatg ctccctgtat
    19501 tatttttatc ga
//
LOCUS       DASPRR010000296         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_715152, whole genome shotgun sequence.
ACCESSION   DASPRR010000296 DASPRR010000000
VERSION     DASPRR010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715152"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..669)
                     /locus_tag="VEL68_11265"
     CDS             complement(286..669)
                     /locus_tag="VEL68_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXZ36588.1"
                     /translation="MLEGMKKGKGVEALEVFKKSHKLVLDVYRITKEFPTGEKFGLIT
                     QMRRASTSIPANLIEGSERAGRLEYRRFVSIAKGSAGELKYYFLLAKDLGYISAEDYL
                     PFRDQLDEVGKMLSGLVKSLSTAKE"
     gene            complement(723..2093)
                     /locus_tag="VEL68_11270"
     CDS             complement(723..2093)
                     /locus_tag="VEL68_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ36589.1"
                     /translation="MLAKPIIQELQNMVNKDRCLTAPEDLLVYSHDTYIGKKPDVVVL
                     PATTEEVSKIMKLATRERIPVTPRASATGLTGMCVPEHGGIVMTMSKMNRILEVNPED
                     RLAVVEPGVITNDLQMAAEAAGVFYPPDPSSLTICQIGGNVATNAGGPRCVKYGVTRD
                     YLLGLEAVLPSGEVIKSGGRPIKNVTGYDVTRLLCGSEGTLAVITKIIVKLIAKPEAR
                     RTLLVAFRSIEDASTTVSRIMAAGIIPRALELMDQHYIRLCEKMYHLGLATEAAGLLI
                     IEVDGFAETVDRQARIAKEFCEAQGAFDIKLARTEEESERLWMARRMGSVALFRLSKT
                     MLSEDATVPISKIPAMVAKIKESEKKHEMTVYFLAHAGDGNMHPLLCYDPGNKEEAEK
                     VENIVREIFEASIALGGTLTGEHGIGLAKKPFMPLEISPVELELWKNIKKAFDPNNIM
                     NPGKFV"
     gene            2363..3754
                     /locus_tag="VEL68_11275"
     CDS             2363..3754
                     /locus_tag="VEL68_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ36590.1"
                     /translation="MAVDFDKFFYPKSVVVVGASSAPHKPGNDVIRNILANGFGGNLY
                     LVNPKGEEILGMKAYSSIQDLPEGIDLAVVILPAALNPQAIRDCAAKGIQAVVLAAGG
                     FSEVDEKGEELQENLTRAVRETGVRVIGPNTSGHTSTPHHFTSTFFPQGKIPRGPVSY
                     IAQTGNFATHTMRYITTGENFGVARVVGLGNKVDIEESDVLEYYAGDPETRAIFIYLE
                     SFKNPRRFLSIARELTRKKPVFLLKGGATAEGAKAAVAHTAAMAAEDRVIDGALKQAG
                     IARIYQYSHLFLAAKALASMPLPRGNRVSFLAPSGAMLVVLTDLCLSLGLKVPPFEES
                     TRHFLQSISPPIVRMRNPVDIWFSAGIHGVEFAYREGTEAALKDPNIDAVVPILLLTD
                     ETGVPPLDFLVDLAKKYPEKPIYVTFTGEKKHMDAGKAFLEPKGVPTFPLIEEPFEVL
                     SLLARCRRFMDHN"
     gene            3922..4875
                     /locus_tag="VEL68_11280"
     CDS             3922..4875
                     /locus_tag="VEL68_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ36591.1"
                     /translation="MSNKALVVLATVALSFLLLSSPALAQKIDLKLMTGPMGGSWYPL
                     GGAIGEALQKEIPGLTISVMPGGGVANVEAVEFGKCELGFSNSSSAVDGLNGRAPFKK
                     KTENVKQLANLYAQYFQMVVVADSPIKSVADFKGKIIATTPKGHTGTILTEQVLQVYG
                     MSFKDISKVHFVGYSDAVALMKDGHADIYSPGTTIPASSIIDLAADRKIRLIGLPDDK
                     IKALQQINAGYLKRTTPKGTYPGVDYDVPGVGYFAHLVVSAKLPDDLVYKITKTFAKN
                     LPRFADVVKDMKGVTPKDLALDIGVPFHPGALKYYKEAGVL"
BASE COUNT         1085 a         1448 c         1329 g         1183 t
ORIGIN      
        1 tccgtgtaat cggaataggg aagttcaccc gctgccaggg cccgggcaac ctccgtcttt
       61 ccccgggagc tgtaggtctc cctctgcagc tcgcgaaaaa ccgggcagac tgccgcacag
      121 accccgcatc cagagcattt ctcggcttcc tggaggatga attgggttga gtacatggaa
      181 actccgtttg agtgaaagtg caagtgaaag tgaaagtacc ctttggaaat atactttgaa
      241 cgaaagatcg accgaaagta aaaatagggc ttcagaatac tttttttatt ccttggcggt
      301 ggataacgat tttacaaggc cgctaagcat cttaccaact tcgtccaatt ggtccctgaa
      361 agggagataa tcttcggcgg aaatatatcc taaatctttc gccagcaaaa aataatactt
      421 aagctcgcca gctgaccctt tagcgatgct tacaaagcgg cggtattcga gccttcccgc
      481 cctctcgctt ccctctatca aattcgcagg aatcgaggta gatgctcgcc tcatctgagt
      541 aataaggcca aatttctctc ctgttggaaa ctccttcgta attctataga cgtccagtac
      601 aagtttatgg ctcttcttaa atacctccag ggcttcaaca cctttgcctt ttttcatccc
      661 ctcaagcatt ggttctctcg agttttattt ttctttcctt ttcacttaca ctttcactgc
      721 ctttaaacga actttcccgg attcatgatg ttgttcgggt cgaaggcctt cttgatgttc
      781 ttccagagct ccagctctac cgggctgatt tccaggggca tgaagggctt cttggccagg
      841 ccgatgccgt gctccccggt gagcgttccc cccagggcga tggaagcctc gaagatttcc
      901 cggacgatat tttccacttt ttccgcttct tctttgttcc ccggatcgta gcaaaggagc
      961 gggtgcatgt tgccatcccc ggcatgggcc aggaagtaga cggtcatctc atgtttcttc
     1021 tcgctttctt tgatcttcgc caccatggcc gggatcttgc tgatggggac ggtggcatct
     1081 tccgaaagca tggttttgct gaggcggaag agggccacgg aacccatcct ccgggccatc
     1141 cagaggcgct ctgactcttc ttcggtgcgg gctaatttga tatcgaaagc cccctgggcc
     1201 tcgcagaatt ccttggcgat cctcgcctga cgatctaccg tctcggcaaa gccatccacc
     1261 tcgatgatga gcaagccggc ggcctcggtg gccagtccca gatgatacat tttctcgcag
     1321 agccggatgt agtgctggtc catcagctcc agagcgcggg gaatgatgcc cgcagccatg
     1381 atacgggaaa cggtggtgct ggcgtcttcg atggagcgga aagccaccag cagggtgcgc
     1441 cgggcctcgg gcttggcgat gagcttgacg atgatcttgg tgatcaccgc cagggttcct
     1501 tccgagccgc acaggagacg ggttacgtcg tatccggtta cgttcttgat cggcctcccg
     1561 ccgctcttga tgacttctcc cgaaggcagc acggcctcca accccaggag atagtcccgg
     1621 gtcaccccgt acttcacgca cctcggcccc ccggcgttgg tggccacgtt gcccccgatc
     1681 tggcagatgg tcaggctcga cgggtccggg ggatagaaga ctccggccgc ctccgcagcc
     1741 atctgcaggt cgttggtaat gaccccgggc tcgaccaccg ccagccggtc ctccgggttc
     1801 acctccagga tcctgttcat cttggacatg gtcatgacaa tcccgccgtg ttccgggacg
     1861 cacatgccgg tcagccccgt ggccgacgcg cggggggtga ccggaattct ctcccgggta
     1921 gccagcttca ttattttcga gacttcctcc gtggtggcgg gcaacacgac gacatccggc
     1981 ttcttcccta tgtaggtgtc atgggaataa actaggaggt cttccggggc ggtgaggcag
     2041 cggtctttgt tcaccatgtt ttgaagttcc tggatgattg gttttgccag catggacctt
     2101 ctccttgtat aatgtctttt gcagggtcgt tgatgatttc cctacgagtt gtcattctcc
     2161 cgcaggcgag aatccaggac gcatgaggga ccttgaagaa actggattcc cgttttcacg
     2221 gaaatgacgg aaaggcaaaa atttttacca aaccctcatg gttaagtccg tccttgttta
     2281 atcccttcgc attttattat attccggacg gatataccag aaatgattcg gatcattttg
     2341 cgcgaggcag aaaggagttt tcatggccgt cgattttgat aaattcttct atccgaaaag
     2401 cgtggtggtc gtcggggcct cttcggcccc ccacaagcct ggcaatgatg taatccggaa
     2461 tattctggcc aatggatttg gaggcaatct ctacctggtg aaccccaaag gggaagagat
     2521 cctgggcatg aaggcctatt cctccatcca ggacctcccc gaaggaatcg acctggcggt
     2581 ggtcatactg cctgccgccc tgaacccgca ggccatccgg gattgcgccg ccaaggggat
     2641 tcaagctgtt gtccttgcgg ccggcggttt ctccgaggtg gatgagaaag gcgaagagtt
     2701 gcaggaaaac ctgacccggg cggtgcgtga aaccggcgtg cgggtcatcg ggcccaacac
     2761 ctccggccac acttccacgc cccatcattt tacctccact tttttccccc aggggaaaat
     2821 cccccggggc cccgtttcct acattgccca aaccggaaac tttgccactc acaccatgcg
     2881 gtacattaca accggggaga acttcggagt ggcccgtgtg gtcggcctgg ggaacaaggt
     2941 agacatcgag gaaagcgacg ttctggagta ttacgctggc gaccccgaga ccagagcgat
     3001 tttcatctac ctcgaatcct tcaagaaccc ccgccgcttt ctttccatcg cccgggagtt
     3061 gacccgaaag aaacccgtct ttctcctgaa aggaggggcc accgccgaag gggccaaagc
     3121 cgccgtagcc cacaccgcgg ctatggccgc agaggatcga gtcattgacg gagcgctgaa
     3181 acaggccggg atcgctcgta tctaccagta ctcccacctt tttctcgccg ccaaggctct
     3241 tgcttccatg ccccttccga gaggaaaccg ggtgagcttt ctggccccct caggggccat
     3301 gctcgtggtc ctgactgacc tgtgcctgtc cctgggccta aaggttcccc cctttgagga
     3361 gtccacccgt cactttcttc aaagcatcag tccccccatt gtccgcatgc ggaaccctgt
     3421 ggatatctgg ttctctgccg ggattcacgg agtcgagttc gcctaccggg aaggaaccga
     3481 ggctgctctg aaggatccca acatcgacgc cgtggttccc atcctgctgc tgaccgatga
     3541 gacgggcgtt cctcctctgg attttctcgt agacctagcc aagaaatacc ccgaaaagcc
     3601 gatttatgta acctttaccg gcgagaagaa gcacatggac gcggggaaag cattccttga
     3661 gcctaaggga gttcccacct ttccccttat cgaggaacct tttgaggtcc tctccctcct
     3721 ggcccgatgt cggaggttta tggaccacaa ctaaaagcgc ataaggttgg aggcagaagc
     3781 gcccttaggc atggtcccaa tgcagattaa gaatggaact gggcgggtga caaaagtgat
     3841 tgatttgtga acattttctt gttatttttc tcactccggt gtacctcgga accataggca
     3901 aaaccaaaaa gggaggtaaa catgtcaaat aaagctcttg ttgttctggc gactgtcgct
     3961 ctgtcgtttc ttcttctatc atctcctgct ctcgctcaga aaatcgactt gaaattgatg
     4021 actgggccca tgggagggtc ctggtatccc ctgggcggag caatcgggga agccttgcag
     4081 aaagagattc ccggattgac catatcggtc atgcccggcg ggggagtagc caatgtggaa
     4141 gccgtggaat ttggaaaatg tgagctgggg ttctccaact ccagttcggc ggtggatggc
     4201 ctgaacggcc gagccccttt caaaaagaag acggaaaacg tgaaacaact ggcaaacctc
     4261 tatgcccaat acttccagat ggtcgtggtg gccgattccc ccatcaaatc cgtggcggat
     4321 ttcaagggaa agatcatcgc cacgaccccc aaaggtcata cgggaaccat tttgacggaa
     4381 caggtcttac aggtttacgg catgtctttc aaagacatat ccaaggtcca tttcgtcggc
     4441 tacagcgacg ccgtggccct gatgaaggac ggccatgcgg acatttattc tcccggcacg
     4501 accattcccg cctcctcgat catcgacctg gccgctgacc ggaagatccg tctcattggg
     4561 ctgcccgatg acaagatcaa agccttgcag cagatcaacg ccggttatct gaagagaacc
     4621 acccccaagg gaacctatcc cggtgtggac tacgacgtgc cgggggtcgg ttattttgcc
     4681 cacctggtcg tcagcgcgaa gcttcccgat gatttggttt acaagatcac caagaccttc
     4741 gccaagaacc tcccccgctt cgccgatgtg gtcaaggaca tgaagggagt caccccgaaa
     4801 gacctcgccc tggatatcgg agttcctttc catcccgggg cgctgaagta ttacaaagaa
     4861 gccggtgttc tgtgataacc ttgaataaag aatgtagggg ccagcggccg ctcgccccta
     4921 caacggtcaa acttctgtgg cacgcggccg cgccctccta caaactatac acggcgtgct
     4981 aattcgaacc cctggcggca ggggtaaatg ccaaacgccc aaatcataga ttgttcagac
     5041 ttgcc
//
LOCUS       DASPRR010000297        11138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1679618, whole genome shotgun sequence.
ACCESSION   DASPRR010000297 DASPRR010000000
VERSION     DASPRR010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11138)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679618"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VEL68_11285"
     CDS             complement(<1..769)
                     /locus_tag="VEL68_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36592.1"
                     /translation="MKPSRPVLSVAIGTVLFLLTFAGASFAQDTLKVGAMFSLTGAGS
                     AVGKIQVEVVKLAVKDVNDQGGVSVGGKKMKIEAVIRDDETKPDVAVRRLREYQNDGI
                     NLLVAGTFAHVSTALNEQVKGGQTFIIVPNGIEEKVFEKKEKAPYFSSTLGCVDAIGR
                     IIGDYVCKTYKPKHVMLFLPDYAYGHGAARGAKRYFKEKQPDIKLSELWSPVGTPDFT
                     SYIIKVKEAKPDVVMMGHWGNDAINVLKQVYELGLRKE"
     gene            complement(1132..1515)
                     /locus_tag="VEL68_11290"
     CDS             complement(1132..1515)
                     /locus_tag="VEL68_11290"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HXZ36593.1"
                     /translation="MQVMQEWLGKVDPLMIKAVGPMAGGSRVGSLCGALQAGVLTLGM
                     LYGAEGEKLSSQEALVESFQPVIRFYREFEKAFGSRNCPEIIHADLNNPEERKKWVDR
                     GGLEECAHLCGQTVRLLFAVIGEKG"
     gene            complement(1790..2659)
                     /locus_tag="VEL68_11295"
     CDS             complement(1790..2659)
                     /locus_tag="VEL68_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ36594.1"
                     /translation="MSEPFGKIIRGDGIGIHLALWEGRGKDILAVHGLTANCRCWDAI
                     ARSVAPRHRFLAMDLRGRGLSDKPAAGYSIDIHARDILYLLDHLGLKQVVLMGHSLGA
                     FISLVFAARYPERVTRLILVDGGGKLNADHTARVFAGIKTSLERLGKVFSSFDAYAGI
                     LRKAPFLQPWSQALENYFRYEVENVPGGVRPRIRPETIQEEIVNLTRLDVSHFYPQVH
                     CPVLILRATEGVLYPDDILLPEDVVQRMVREIPKARRVDVEKTNHYSILFQDNPARDQ
                     AILQFLEEDGKLG"
     gene            2847..3041
                     /locus_tag="VEL68_11300"
     CDS             2847..3041
                     /locus_tag="VEL68_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXZ36595.1"
                     /translation="MMNLGFFELMIILAIVFFIFGGRKTSPISKGFGLAIRNFKKGLH
                     EPDAIDVTPKKETPPEEPPQ"
     gene            complement(3604..4251)
                     /gene="cybH"
                     /locus_tag="VEL68_11305"
     CDS             complement(3604..4251)
                     /gene="cybH"
                     /locus_tag="VEL68_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HXZ36596.1"
                     /translation="MPKRVYVWEWPVRLTHWLNVYCIGILCFTGFYIGSPFIQASISD
                     EYPIMAIMRLIHFTTAYFFTMSFFIRIYWAFGGNEYAKVGEFIPVTRKVWGDLWGDVK
                     FYLFLTKKHGARAGHHTLAGITYVLLFIFFHFEIVTGFALYSESHSGFISVVMGGWLL
                     NYFSPQSLRLYHHLVMWLGIIFAMTHIYVGWYNDVVERNSIMSSIFSGYKTLDKE"
     gene            complement(4261..5969)
                     /locus_tag="VEL68_11310"
                     /pseudo
     CDS             complement(4261..5969)
                     /locus_tag="VEL68_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992146.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
     gene            complement(5973..7082)
                     /locus_tag="VEL68_11315"
     CDS             complement(5973..7082)
                     /locus_tag="VEL68_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HXZ36597.1"
                     /translation="MEEESLVQELTQRGVSRRDFLKFCTTMAAALALPASMVPRIAEA
                     LEKKAKPVVLWLEFSDCAGDSESMLRATKPTVSEIVLDVISLEYHETIMAPSGMAAEK
                     SKKDVVKNLKGKYIAIVEGAIPMKDGGVYCTIGGHTALDIAREVCGNALATLAIGSCA
                     SWGGVGSASPNPTGCVGVKEAVPGATVVNLPGCPVNVENVTATIVHFLTFGSLPALDS
                     VGRPLFGYGKRIHDNCERRPHFDAGQYVRQWGDEGHRLGWCLYEMGCKGPQAHYNCPT
                     VKYNAGTSWPVQAGHPCFACAANNNWDSMGPIYSRLPDVPGAGYQATADKVGLVLTGL
                     TGAGIAAHAIVKGIQATASKEILKHEDKEAEKKGE"
     gene            7724..10783
                     /gene="fdnG"
                     /locus_tag="VEL68_11320"
     CDS             7724..10783
                     /gene="fdnG"
                     /locus_tag="VEL68_11320"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:8279..8281,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HXZ36598.1"
                     /translation="MEVSRRGFLKISGATALAGGLGLSAQPASAQGPPPKILYTRETT
                     TICPYCAVGCGIIVSTRDGKVINTEGDPDHPINQGSLCPKGSSLYQLSDNPNRLKTPL
                     YRAPYAKEWKEVSWDFTLDKIADHIKTVRDASFMQKNEKGETVNRTDAIASVGSAAMD
                     NEECYVYQKFLRGLGLVYIEHQARIUHSPTVAALGESFGRGAMTNHWNDIANADVILI
                     MGSNPAENHPISFRWINRAIEKGAKLLCVDPRFTKSAAKAHIYAPLRSGTDIAFLGGM
                     IKHILENDLIQKEYVVEYTNASFLIDEKFGFQDGLFSGYDAKGRKYDRSTWKYQLDAN
                     GIPKMDKTLQSPRCVYQLLKKHYDRYNLDIVSQITGTSKDDLGKVYQAYAETGKPDKV
                     GTILYAMGWTQHTVGTQNIRAMSIIQLLLGNMGMAGGGVNAMRGESNVQGSTDYGLLF
                     HILTGYNPAPRAADKTLNEYNEQYTPKTKDPKSFNWWKNRPKYITSFLKAMYGDAATK
                     ENDFGYGWLPKLDATQNGSWLVLFDQMAKGKIKGFFAWGQNPACSGANANKVRKALEN
                     LDWLVNVNLFDNETGSFWKGPGKDPSKIKTEVFMLPCAASFEKEGSLSNSGRWMQWRY
                     KAVNPPGEAQPDLEIMHELFLKVRKLYKEKGGIFPEPILNLSWNYGLKGADGMVHHIN
                     PHFVAREINGYTAKDIEVEDPKTKAKTVVVKAGQQLPSFAFLQDDGSTTCGNWLYCAS
                     YTDAGNMAARRGKADPTGLMLYPFWAWCWPVNRRIIYNRASVNWEGKPWDPKRAILWW
                     DGSKWMGDIPDGPPPPGAIYPFIMLPEGHARLFGLAMAEGPFPEHYEPLECPIEKNLM
                     SSQKFNPTARIFGEAGVGVGADKYLSCDPRFPLVCTTYRVTEHWQTGVMTRWTPWLVE
                     LEPQMFVEMSKELAKEKGIQGGEQVVVKSARGEVDAKAIITERFKPFKIAGQTVHQVG
                     IPWHYGWVTPKDGGDSANLLTPTIGDANTMIPESKAFMVNVEKKGGK"
     gene            10785..>11138
                     /locus_tag="VEL68_11325"
     CDS             10785..>11138
                     /locus_tag="VEL68_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ36599.1"
                     /translation="MEEKVLLVDVSKCTACRACQVACKQWNRLPAEKSEQRGTHQNPP
                     DLTWATWNLIRFTEVSNKEGAMKWLFRRDACLHCTDPGCQRACPVPGCITKTPEGAVF
                     INQELCIGCKMCVNAC"
BASE COUNT         2573 a         3212 c         2976 g         2377 t
ORIGIN      
        1 attcctttct caaacccaat tcatacacct gcttcaggac attgatggcg tcgttgcccc
       61 agtggcccat catgaccaca tcgggtttgg cctctttgac cttgatgata taggaggtga
      121 aatccggcgt gcccaccgga gaccagagtt cgctgagttt gatgtccggc tgtttctcct
      181 tgaaatacct tttcgcccct cttgccgccc catggccata ggcgtaatcc gggagaaaga
      241 gcatgacgtg tttgggcttg taggtcttgc acacataatc cccgatgatt ctcccgatgg
      301 catccacgca gcccaaggtg gaggagaaat agggggcttt ctctttcttt tcgaagacct
      361 tctcctcgat tccgttgggg acgatgatga aggtctgtcc gcctttgacc tgctcgttca
      421 gggcggtgct cacatgggcg aaagttccgg cgaccaggag attgatgcca tcgttctggt
      481 attcccgcaa tctgcgcacc gccacatccg gtttggtctc gtcatctcga atgaccgcct
      541 cgatcttcat tttcttgccg cctacactta cgcccccttg gtcattcacg tctttcacag
      601 ccaatttgac tacttccact tggatctttc ctactgctga ccctgcgccc gtcaggctga
      661 acatagcccc cactttcagg gtatcctggg caaaggaagc tcctgcgaat gtcagcagga
      721 aaagaacggt tcctattgct actgacaaca cgggtctact cggcttcatt ttttcctcct
      781 ttgtcgttag aaatcttgct tagtgcccga tcttaccctt tcacctcaaa gcctggaagc
      841 aaatgcactc tggatcatac tccatccgtt tactgaaagt gaatatagaa aaatagaggc
      901 cgagtgtcaa gggggaacag aacttgataa attcctaaaa gtcgaaattc aatcgcgggc
      961 gtcgttccgc cgaaacttgt cccggcaggt actaagccgg gagccggaat cccagttaga
     1021 agattttctg gaccccggcc ttcgccgggt gaggattcaa ccttaagaaa agccttttaa
     1081 tgactgtcaa gttttaaatg aacctgcaag aatccttttg gtttcaaatc cttaaccctt
     1141 ttccccgatg actgcaaata aaaggcggac ggtctgcccg cacagatggg cgcattcctc
     1201 gaggcccccc cggtcgaccc atttctttct ctcctcgggg ttgttcaggt ccgcgtggat
     1261 gatctccggg cagtttcggc tgccgaaggc tttctcgaat tcacgataga aacggatcac
     1321 cggctggaag gactccacca aagcctcctg gctgctcagc ttttcgcctt ccgctccgta
     1381 gagcatcccc aaagtcaaaa cccctgcctg caaagccccg cacagcgacc ccacccggct
     1441 cccgccggcc atgggcccca cggccttgat catcagaggg tccactttcc ccaaccactc
     1501 ctgcatgacc tgcaggacgg tctgcgatca ggcccgcctt tggccggttt tcaagagagt
     1561 gaatgctttt tgcgcggcct catcaatgat ctgcccccgg gctttcatct tccctcctta
     1621 atcaaggatt ccttggattt ctttcttttt gaggttcgaa atacgaactc ttcatccgca
     1681 gattttgcag atcacgcaga gatgggttac gaatcatcct gaaacctctg cggcccctgc
     1741 gggctctgcg agaaaaatat tttgggtttt gtctttgtgg cgctgtaaat tatcccaatt
     1801 tgccatcctc ctccaagaat tgaaggatgg cttggtccct tgccgggttg tcctggaaga
     1861 gaatcgagta atggttcgtc ttttccacgt cgactcgcct tgctttcgga atttccctca
     1921 ccatcctttg caccacatct tcgggtagaa gaatatcgtc ggggtagagg accccttccg
     1981 tcgcccggag aattaaaacc gggcagtgga cttgagggta gaagtgcgag acatccaggc
     2041 gggtcaggtt cacaatctct tcctgaatgg tctccggacg gatgcgaggg cgaactcctc
     2101 ccggaacatt ttcgacctca tagcggaagt aattctccag ggcctgggac cagggctgca
     2161 gaaaaggggc cttcctcagg atgcccgcat aagcatcgaa ggaagaaaag actttcccca
     2221 gacgctccaa ggaggttttg atcccggcaa acactctggc ggtatggtcg gcgttgagtt
     2281 ttcctccccc atccaccagg atcaagcggg ttaccctctc aggataccgg gcggcgaaca
     2341 ccagggagat gaaggccccc agggaatggc ccataagtac aacctgtttc aagcccaaat
     2401 gatccaggag gtacagaatg tcccgagcgt gaatgtcgat ggaatacccg gctgcgggtt
     2461 tgtccgagag accccgtccc cgaaggtcca tggccaggaa acgatggcgt ggcgccaccg
     2521 aacgggcaat cgcgtcccag caccggcagt tcgcggtcaa cccgtgaacg gccaaaatgt
     2581 ctttcccccg tccctcccac agggccagat ggattccgat tccatctccg cgaattattt
     2641 ttccaaacgg ttcagacatt ctatcctctt cccgctcagg ccggtttttt tcaagaagaa
     2701 aacaaactct cttctgccgg ccagctccca cgtatcatat catttctggc ttacctccgc
     2761 catgacccgc aaaatgtgct atgatcaaaa caaaatcaac gaaccctttc cttttaccag
     2821 cgcggcgggg ggcgcgggag aattccatga tgaacctggg cttttttgaa ctgatgatca
     2881 ttctggccat cgtcttcttc atcttcggtg ggaggaaaac ctctccgatc agcaagggat
     2941 tcggcctggc catccgcaat ttcaaaaaag gcctccacga gcccgacgcc atcgacgtta
     3001 cccccaagaa ggaaacccct ccagaagaac ctccccaata gccacagcgc gggggagccg
     3061 caaccgaagt ccctagaatg cccaaggccc caaaaaaaga aaaattattc ccttgaaaat
     3121 agccaagcgc tttggttttt ctctgccgag aatagcgctt cgcttgttgg gggcgattcg
     3181 ggaggcttcg cgggcatgaa ggctgtgctt aaaatttggc tggatcagga cgggaaggca
     3241 ttggaggatc gaccttacga tcttttgaag agaattgaag cgacaggatt tcttcataaa
     3301 gatacaaagc agatggagat ggccaacagc caagcatgga agctgactcg gatgatagaa
     3361 taaggcttgg ctttagggga ctggaacggg aggtaggagg ccgatttgga ggaggctcac
     3421 gaattacccc acgagcaaaa ggtctgatgt cttgctatgc gctattttga gaggaaactc
     3481 accaagcagt gaatcgaatt tctataaaac gatttcctcc attcgaggag ccaagatcaa
     3541 gcggcaagcc caggtccaca aagcaaaaag gcaaaccccc cggttgatgc aaggagggag
     3601 attctattct ttatccagcg tcttgtatcc cgaaaagatc gaactcatga tcgagttccg
     3661 ctccaccaca tcattgtacc acccgacata gatgtgggtc atggcgaaga tgatccccag
     3721 ccacatgacc agatgatggt acagccggag gctttggggg gaaaagtaat tcaagagcca
     3781 tccgcccatg acgacggaaa tgaacccgga gtggctttcg gagtaaagag cgaaaccggt
     3841 gacgatctca aagtggaaaa aaataaaaag gagcacatag gtgattccgg ccagggtatg
     3901 gtggccggcc cgggccccgt gcttcttcgt caggaagaga tagaatttca catcccccca
     3961 taaatctccc cagacttttc gggttaccgg aataaattcc ccgaccttgg catattcatt
     4021 ccccccaaag gcccaataga tccggatgaa aaagctcatc gtgaagaaat aggccgtggt
     4081 gaagtgaatc aacctcatga tggccatgat ggggtattca tccgatatgg aagcctggat
     4141 aaaggggctg ccaatgtaaa accccgtaaa acagaggatt ccgatacaat acacattcag
     4201 ccagtgggtc agccgcaccg gccattccca tacgtacact ctcttgggca tagagacctc
     4261 ctacagggcc cgaaccctga agaactcctt tcgttctgaa tcgaccaggt ggatggcgca
     4321 ggccatacag ggatcgaagg agtgaatggt ccggaggatt tccagaggtt tatcgggatc
     4381 ggccaaaggc gtccccacca gcgcttcctc ataggggcct cttttcccct tggcatcccg
     4441 gggagcggag ttccatcctc ccgggacgac gcactggtaa ttggccacct tttggtcttt
     4501 gatgacaatc cagtggcaca aaaaaccccg gggggcttca tggaagccaa acccccgggc
     4561 ttccttcggc caggtcgagg ggtcccactt ttccccattg tgaatgcgga ggtccccccc
     4621 cttcatcttt gcggcaagct ccccgatcca ggacggcagc atctccgcgg tcaccagggt
     4681 ttcgattccc cgcgcagcag tgcggcccag agtggagaac agggcttccg ggccagcccc
     4741 caattttttc agcactccat cggtcacttc cttgatcctc ggatgtccgg aggcataagc
     4801 gaccagcatc cgggaaagag gccccacttc cgtgggcaaa ccgtcatacc gatgcctttc
     4861 acccaggtgt acttcttggc cgtatccaga aactcgtagg gaggggtcgg tccggtgtag
     4921 ttgggaaccg tctcgccgtc ccagggatgt ttggccttat catctccatc gctgtactta
     4981 taccaggcgt gagccgcgtc ttcggtcact ttcttgtggt cgatcggttc tattttgccc
     5041 aggttcttgt tccggatgaa accggcggga agccagcaat tggcggtgtt actctggtta
     5101 tccttctgga attctccaca ggaaagatag ttgcccactc cccccccgat ggctgcccac
     5161 tccttgtaga atgaggccac ggccaggagg tcgggaatgt aaaccttctg gacgaactcc
     5221 agcgcttttt tggccagccc ctgcatgaaa gccaggctcc cggcgttcag agcattctgg
     5281 ctattggggt caaccgggac ggacatccct cccactagga aggtctgggg gtgtgggttc
     5341 ttagccccca agagggcatg catccggatc acgtccctct gccagtcgag ggcttcgaga
     5401 taatgggcga cagccatgag gttggcctcg ggaggcagtt tgtaggcggg atgcccccaa
     5461 tatccattgg caaagatgcc caattgccca ctctccacca gggttcggac cttcccctgg
     5521 accgccttga agtggctggg gctggaggtg ggccaatcag acagggattg ggcgagggag
     5581 gcggtcttgg ccgggtcggc ttttaaagcg ctgactacat cgacccaatc cagcgcatgg
     5641 aggtgataga aatggatcac gtgatcctgg acgtattgga ttcccgaaat caagttgcgg
     5701 ataatccggg cattgtccgg aatggtgatc cccaaggcgt tttcgacagc tctcacggag
     5761 ctggtggaat gaaccgttgt tcaaacccct catatccggg agaggaaggc ccaaacctcc
     5821 ctcgggtccc ggttcttgac gatgatttcg atccccctcc acatggtgcc ggaggaccac
     5881 gcatccatta ccttcccgcc gtcaacctgc gcttcaatcc ggagatgacc ttcgatccgg
     5941 gtgatgggat caatcgctat cttcggcatg ccttattctc ctttcttttc agcttcttta
     6001 tcttcatgtt tcaaaatttc tttggaagca gttgcctgaa tccccttgac aatcgcatgg
     6061 gcggcgattc ctgcgccggt caagccggtg agaaccaatc ccaccttgtc ggccgtggcc
     6121 tggtatcctg cacccggcac atcgggtaga cggctgtaaa tcggccccat gctgtcccag
     6181 ttgttgttgg ccgcgcaggc gaagcaggga tgccctgcct ggaccggcca gctcgtaccc
     6241 gcattgtatt ttaccgtagg acagttatag tgagcctgcg gccccttgca tcccatctcg
     6301 tacaagcacc accccagccg atggccttca tctccccatt ggcgtacgta ctgcccggcg
     6361 tcgaaatggg ggcgcctctc gcagttgtcg tggatcctct tgccatagcc aaagagaggt
     6421 cgtcccacac tgtccaaggc gggaagggac ccgaaggtca ggaaatgaac gatcgtggcg
     6481 gtgacgttct ccacgttgac cggacatccc ggaaggttga caaccgtggc tcccggaaca
     6541 gcctctttga cccctacaca ccccgtgggg ttcggagaag cgctgcccac gcccccccag
     6601 gaagcgcagg agccgatcgc cagcgtcgcc agggcattcc cgcagacttc ccgggcgatg
     6661 tctaaggccg tatgtccacc gatggtgcaa tagaccccac catccttcat gggaatggcc
     6721 ccttccacaa tcgcgatgta ttttcccttg agattcttga ccacgtcctt cttggatttc
     6781 tccgcagcca tccccgaggg ggccatgatg gtctcatggt actccagaga aatcacatcc
     6841 aggacgatct cggagacggt cggcttggtg gctcggagca tggactccga atcgcctgcg
     6901 caatcggaaa actccagcca taagacgacg ggcttggcct tcttctccag agcttccgca
     6961 attcgaggaa ccatggaagc tggaagagcc aaggcagcgg ccatagtcgt gcagaatttc
     7021 agaaaatcgc ggcgggatac ccctcgttga gtcaattctt ggaccaagct ctcttcttcc
     7081 atgaaagcgc caacctccta ttttttttta atagatttat tacagcccaa agccaaagtc
     7141 aataaaaaag attaatacaa ataataataa aaaattacat ttaaccttaa aaaatacttc
     7201 gaatcaggaa ggaaaacccg cgaccgcata gaaatgattc atgattacgg gaccaaaagc
     7261 ttgacaattg cccttcaagt tgatattcaa aacggtattt gaggaaagga ttttcgatcg
     7321 ctcgtttggc cctctcgacg aaagacagtt tgggcttttt caggcataaa gaaccgaaaa
     7381 gaaaaatccg aacattgacc acattgacct tcctgaaaaa tctactgtcg ctttcgcgct
     7441 ctccgaggac ccggccaaaa gaacagcccg actgaaaaaa aggaggccga accggcaggg
     7501 aaggtctgcg ggcgggatcg atttggccct gcgggaaaga aattcagctc acttcccttg
     7561 gggctcctgg aaagggaatc tacttcccca gcgagccggt taaccggcgg ggaggtcggt
     7621 gaattttctt gagtttcatc ggaaggaggt ggaaggttcg tcggattcag ataggttgtc
     7681 aatcgaatcg ttcgatttta acgaaggagg aaggaggaaa gctatggagg tcagcagaag
     7741 gggtttcctg aaaatttcag gagccacggc tttggccggc ggtctgggcc tttccgccca
     7801 gccagcctcg gcccaggggc cgccccccaa gattctttac acccgcgaga cgaccaccat
     7861 ctgcccttac tgcgcggtgg gctgcgggat catcgtatcc acccgggacg gcaaggtgat
     7921 caacaccgaa ggagaccccg atcatcccat caaccaggga tccctctgtc ccaagggatc
     7981 ttcgctttac cagctttcgg acaacccgaa tcgcctgaag acgcccctct accgggcgcc
     8041 gtacgccaag gaatggaaag aggtctcctg ggattttacg ctggataaga tcgccgatca
     8101 tatcaagacc gtccgcgacg cttcgttcat gcagaagaac gaaaaaggcg agacggtgaa
     8161 ccgcacggac gccatcgcct cggtggggag tgcggccatg gacaatgagg aatgctacgt
     8221 ctaccagaaa ttcctccgcg gcctgggcct ggtgtacatc gaacaccagg ccaggatatg
     8281 acactccccc acggtagcgg ctctgggaga gtcgttcgga cggggagcga tgaccaacca
     8341 ttggaacgat attgccaatg cggacgtcat cttgatcatg ggcagcaacc cggcggagaa
     8401 ccaccccatt tctttccgct ggatcaatag ggctatcgaa aagggagcca agctcctctg
     8461 cgtagacccc cggttcacca aatccgcggc caaggcgcac atctacgccc ccctgcggtc
     8521 gggaacggac atcgctttcc tgggggggat gatcaagcac attctggaga acgacttgat
     8581 ccagaaggaa tacgtggtgg aatataccaa cgcctccttc ctgattgatg aaaagtttgg
     8641 atttcaagac ggccttttct ccggctatga cgctaagggc cggaaatacg accgttcgac
     8701 ctggaaatac cagctggacg ccaacgggat tcccaaaatg gacaagactt tgcagagtcc
     8761 ccgctgcgtc taccaactcc tgaagaagca ctacgatcgg tacaacctgg acatcgtctc
     8821 ccagatcacc ggcacttcca aggacgacct gggaaaggtc taccaggcct atgccgagac
     8881 cggcaagccg gacaaggtgg ggaccatcct ttatgccatg gggtggactc agcacacggt
     8941 gggtacccag aacatccggg ccatgtctat catccagctc ctcctcggaa acatgggaat
     9001 ggcgggcgga ggagtgaacg ccatgcgcgg cgagtccaac gtccaggggt ccacggacta
     9061 cgggctcctc ttccacatcc tgaccggtta caatcctgcc ccccgggctg cggacaagac
     9121 cctgaacgag tacaacgaac agtatacccc caagaccaag gaccccaaat ccttcaactg
     9181 gtggaagaac cggcccaagt acatcaccag cttcctcaag gccatgtacg gggatgccgc
     9241 caccaaggag aacgatttcg ggtacggctg gcttcccaag ctggatgcca cgcagaacgg
     9301 ttcctggctg gtgctcttcg atcagatggc caagggaaag atcaaaggct tttttgcctg
     9361 ggggcagaac ccggcttgct ccggggccaa cgccaataaa gtgcgcaagg ccctggagaa
     9421 cctggactgg ctggtcaacg tcaacctctt cgataacgag accgggtctt tctggaaggg
     9481 tccggggaag gatccttcca agatcaaaac cgaagtcttc atgctgccct gcgcggcttc
     9541 ttttgaaaag gaaggctccc tctccaattc cgggcgatgg atgcagtggc gctacaaggc
     9601 ggtcaacccc ccgggagaag cccagcccga cctggagatc atgcatgagc tcttcctcaa
     9661 ggtccgcaag ctctacaagg aaaaaggcgg cattttcccc gagcctatcc tgaatctttc
     9721 ctggaactat ggcttgaaag gcgccgatgg gatggttcat cacatcaatc cccacttcgt
     9781 ggcccgggaa atcaacggat acaccgccaa ggacatcgaa gtcgaagacc ccaaaaccaa
     9841 agccaagacc gtggtggtca aggccggaca gcaattgccg agttttgcct tcctgcagga
     9901 cgacggctcc accacctgcg gcaactggct ctattgcgca tcttacaccg atgccgggaa
     9961 tatggccgcc cgcagaggaa aggcggaccc aacggggcta atgctctacc ccttctgggc
    10021 ctggtgctgg ccggtcaacc gccggatcat ctacaaccgc gcttcggtaa actgggaagg
    10081 aaaaccctgg gatcccaagc gggccattct ctggtgggat ggcagtaaat ggatgggaga
    10141 tattccggat gggccccccc ctccgggagc catttatccc ttcatcatgc tgcccgaagg
    10201 tcatgcgcgc ctcttcgggt tggccatggc cgaagggcct ttccccgagc actatgagcc
    10261 cctggagtgc cccatcgaga agaacctcat gtcctcccag aaatttaacc ccacggccag
    10321 gattttcggg gaggccggag tgggcgtggg agcggacaag tacctctcct gcgacccgag
    10381 gttccccctc gtctgcacaa cctatcgggt gaccgaacac tggcagaccg gggttatgac
    10441 ccgctggacc ccctggctgg tcgagttgga gccccagatg ttcgtggaga tgtccaagga
    10501 actggccaag gagaagggca tccagggtgg agagcaggtg gtggtgaaat cggcccgtgg
    10561 agaggtcgat gccaaggcca tcatcaccga gcgcttcaag cccttcaaga tcgccgggca
    10621 gacggttcac caggtcggga ttccctggca ttacggttgg gtcacgccga aggatggagg
    10681 agacagcgcc aacctgctca cccccaccat cggcgacgcc aacaccatga tcccggaatc
    10741 caaggccttc atggtgaacg tggaaaagaa aggggggaag tgagatggaa gaaaaagttt
    10801 tgctcgtcga cgtctccaag tgcacggcct gccgggcctg ccaggtggcc tgcaagcagt
    10861 ggaaccggct gcccgcggaa aagtccgagc agaggggcac ccatcagaac ccccctgacc
    10921 tcacctgggc cacctggaac ttgatccgct tcaccgaagt ctccaacaag gaaggggcca
    10981 tgaaatggct cttccggcgg gatgcctgcc tgcactgcac ggaccccggg tgccagcggg
    11041 cttgcccggt tccgggttgc atcaccaaga cccccgaagg agccgtgttc attaaccagg
    11101 agttgtgcat cgggtgcaag atgtgtgtca acgcctgt
//
LOCUS       DASPRR010000298         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1514858, whole genome shotgun sequence.
ACCESSION   DASPRR010000298 DASPRR010000000
VERSION     DASPRR010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514858"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VEL68_11330"
     CDS             <1..283
                     /locus_tag="VEL68_11330"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXZ36600.1"
                     /translation="YKKYRDKGLVVIGVSLDRKEPQEVKKFLDQLQIEYINVMGDEEV
                     LERYSQVAKLGPIRGIPATFVIDRKGRICQRFLGLTEKGVLEEALQPLL"
     gene            378..590
                     /locus_tag="VEL68_11335"
     CDS             378..590
                     /locus_tag="VEL68_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HXZ36601.1"
                     /translation="MQEIRRRIDKEMNIGEVLEKYPQARKVFQKHFGEGCFNCPGSRQ
                     ESIQFGALMHNKDVNAILEDLNATIK"
     gene            604..774
                     /locus_tag="VEL68_11340"
     CDS             604..774
                     /locus_tag="VEL68_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ36602.1"
                     /translation="MSYKITDDCIMCGTCKEECPAEAISEGDPKYVIDPEKCTDCGTC
                     AEVCPVEACVSE"
     gene            819..1040
                     /locus_tag="VEL68_11345"
     CDS             819..1040
                     /locus_tag="VEL68_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXZ36603.1"
                     /translation="MKDKVQKVIDEIRPALQADGGNIELVEVGEDGVVKVRLMGACGS
                     CPMSQLTLKRGVEARLKAKVPEVKEVVSV"
     gene            complement(1149..3149)
                     /locus_tag="VEL68_11350"
     CDS             complement(1149..3149)
                     /locus_tag="VEL68_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ36604.1"
                     /translation="MLKMLFTPIQVGKMELKNRIVMPAMHFLHSWEGMVLPHHVDYFV
                     ERAKGGAALIIIGGCTIDETSGAINMLSAKDDKFIAGLSGLAKAVQDHGAKIAAQLYH
                     AGRYSHSVLMGGKRSVSSSPIRSRLTGETPRELSIPEIKQTQSNYAMAAARVQRAGFD
                     AVEVIASAGYLISQFLSPIVNRRKDEYGGGIENRMRFGLEVIQEIRRGVGPDFPIIVR
                     VAGNEFMDGGLANEEARSFSKELEKAGATMINVTGGWHETRVPQITMGLPRGGFVYLA
                     QGIKQAVSIPVMACNRISDPILADRILRDGQADLIGFARGLIADPELPQKARQGRFDE
                     INYCIACNQGCFDPIFEQKPQTCLVNARAGAEGKLTLEPASSQKKVMVIGGGPAGMEA
                     ARVAALRGHQVSLYEKTETLGGQLPLAASPPGRAEFLTFVRYLEGQMKKLNVTVHPRT
                     EATPRHVEMEKPDAVILATGADPWVPEIKGVRLPNVVLARDVLSCKADPGKEVVIIGG
                     GAMGLETALFLARKGTIDADTLYFLMFNQAEKPETIQSLLYRGLKKITVIEMLKRIGQ
                     DIGRSTRWTILQDLSRLGVRTLTKAKAKEITDQGVWIDREGREEWIPGDTVVLAAGSR
                     PVDSLYDQIRDRVKEIYVIGDAKSPRKALEAVAEGLDVGRKI"
     gene            complement(3346..>3544)
                     /locus_tag="VEL68_11355"
     CDS             complement(3346..>3544)
                     /locus_tag="VEL68_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36605.1"
                     /translation="IFGMISVGRGGMALTSLEGKGRVTKGVRHMVLEDGLGVLTAKGA
                     VQRAAELAKIIRAGKKALQNA"
BASE COUNT          785 a          972 c         1005 g          782 t
ORIGIN      
        1 ttacaagaaa tacagagata agggactggt ggtcatcggg gtctcccttg accgcaagga
       61 accgcaggaa gtcaagaaat tcctggatca acttcaaatc gaatatatta acgtcatggg
      121 ggatgaggaa gtattggaga gatacagcca ggtcgccaaa ttgggcccca ttcgggggat
      181 ccccgccact ttcgtcatcg accggaaggg ccggatctgc cagcgctttt tggggctgac
      241 cgaaaaggga gtcctggaag aggcccttca gccccttcta tgacgaatat atgatttaga
      301 tcattttttt gatccggcgg aatggtaggg tagggcctag gcgaagcgga gaaagtaagg
      361 aataaatagg aggaaccatg caggagatca gacggaggat cgacaaagag atgaacatag
      421 gcgaggtcct cgagaaatat ccgcaagcgc gcaaagtatt tcagaagcat tttggagagg
      481 gctgcttcaa ctgccccggt tccaggcagg agagcatcca attcggcgcc ctcatgcaca
      541 acaaagacgt caacgccatt ctggaagacc tgaatgcaac gatcaaatag atgaggagga
      601 gagatgagct acaagatcac cgatgactgc atcatgtgtg gaacctgcaa ggaggaatgc
      661 ccggctgagg ccattagcga aggagacccg aagtatgtga tcgacccgga aaagtgcacc
      721 gactgcggaa cctgcgccga agtctgcccg gtagaagcct gcgtttctga ataagaaccc
      781 gagggcataa tttcgaggaa aaccaaatgg aggttgttgt gaaggacaag gttcaaaaag
      841 tcatcgatga aatccgtccc gccctgcagg ccgacggtgg aaatatcgag ctggtagagg
      901 tgggggaaga tggggttgtg aaggtgagac tgatgggggc ctgcggctcc tgccccatgt
      961 cccagctgac tctaaagcgg ggcgttgagg cccgactcaa ggccaaggtc ccagaagtaa
     1021 aagaagtggt gtccgtttaa taaagacagt gtcagtgtcc gtgattagtg tcaggaaagc
     1081 aattcctctt tacaccgaca ctaatcacgg acacggacac tgatcatgtt cctcaacacc
     1141 gagcgcttct atattttcct gcccacgtcc agcccttcgg caactgcctc cagcgccttg
     1201 cggggacttt tggcgtcccc aatcacatag atttctttca cccggtctct gatctgatcg
     1261 taaagagaat caaccggtct ggagccggcg gccagaacta ccgtatcccc cggaatccat
     1321 tcttcccttc cctcccggtc gatccatacc ccttggtcgg taatttcctt ggctttggct
     1381 ttggtcagag tccttacccc caggcgggat aggtcctgaa ggatggtcca gcgggtgctc
     1441 cgcccgatat cctggccgat tctctttaac atttcgatta ccgtaatctt ctttaggccg
     1501 cgatagagga ggctctgaat ggtttccggt ttctccgcct ggttgaacat gaggaaataa
     1561 agggtgtcgg catcgatcgt tcccttgcgc gcgaggaaca gagcggtttc cagccccatg
     1621 gcccctccgc cgatgatgac tacctctttt ccggggtcgg ctttgcagga caaaacatcc
     1681 cgggccaaaa ccacgttggg gaggcggacc cccttgatct cgggcaccca agggtcggct
     1741 ccagtggcca ggatgaccgc atcgggtttt tccatttcca cgtgacgagg ggtcgcctcc
     1801 gtgcgcgggt gtacggtgac gttcagcttt ttcatctgac cctccaggta acggacaaag
     1861 gtgagaaact ccgcccgtcc cgggggagaa gcggccaggg gaagctgtcc tcccagggtt
     1921 tcggtctttt cgtagaggct gacctggtgc ccccgcaggg cggcaaccct tgcggcttcc
     1981 attccagcgg gcccaccgcc aatgaccatg accttttttt gggaggatgc cggctcgagg
     2041 gtaagctttc cttccgctcc ggctcgggca ttgaccaggc aagtttgggg cttctgctcg
     2101 aagatggggt caaaacaacc ctggttgcag gcaatacaat aattgatttc gtcgaaacgt
     2161 ccctgccggg ccttctgggg aagctctggg tcggcgatca ggccgcgggc aaaaccgatc
     2221 aaatccgcct ggccgtctcg caggatccgg tcggctagga tggggtcgct gatccggttg
     2281 caggccatga cgggaataga tacggcctgc ttgattccct gggcgaggta gacgaacccg
     2341 ccccggggca atcccatggt gatctgaggg acccgggtct cgtgccaacc cccggttacg
     2401 ttgatcatgg tcgccccggc tttttccaat tccttggaaa acgaccgggc ttcctcattg
     2461 gccagccccc cgtccataaa ctcgttgccc gccacccgga cgatgatggg gaaatccgga
     2521 cccactcccc ggcggatttc ctggattact tccaggccga aacgcatccg attttcgatg
     2581 cctcccccgt attcgtcttt ccgacggttc acgatggggg acaagaattg gctgatcagg
     2641 taaccggcag aggcaatcac ctctacggca tcgaagcccg cccgctggac tctcgccgca
     2701 gccatggcgt agttgctttg agtctgtttg atctccggaa tcgacaattc ccggggggtc
     2761 tcgccggtga gccgggagcg gatgggggag gacgatacgg atcgctttcc ccccatgagc
     2821 acggagtggg agtagcgccc cgcgtggtag agctgggcgg caatttttgc gccgtgatcc
     2881 tggaccgctt tggcaagccc ggaaagaccg gcgatgaact tgtcgtcctt ggcgctgagc
     2941 atgttgatcg ccccgctggt ttcatcgatg gtgcagcccc cgatgatgat cagggccgcg
     3001 ccccccttcg cccgttcgac gaagtaatcc acgtggtggg gaagcaccat accttcccac
     3061 gaatgcagaa aatgcatggc gggcatgacg atccggttct tcagttccat ctttcccact
     3121 tgtatcggag tgaaaagcat cttcaacatc tatcgtctcc ttttgaacct tagaaaccta
     3181 aaaagggttc ggacccattt tattttgaat ttggaaaaag ggaatcggaa gttgcaagat
     3241 ggaaattgtc aaaaaaattt ataaatcaac tcggccctca cgcttcatag cccttatttt
     3301 ttgacagcga ggaaatcatt ctgggcattt tagggatttc aacctttagg cattctggag
     3361 ggcttttttc cctgcccgga taattttggc cagttccgca gcgcgctgga cagcgccctt
     3421 ggcggttaaa accccaagcc cgtcttccag gaccatgtgc cggacgccct tggtcactct
     3481 tcctttccct tccaggctgg tcagggccat tccgccgcgg cccacggaga tcattccgaa
     3541 gatg
//
LOCUS       DASPRR010000299        13529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_717309, whole genome shotgun sequence.
ACCESSION   DASPRR010000299 DASPRR010000000
VERSION     DASPRR010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717309"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VEL68_11360"
     CDS             <1..253
                     /locus_tag="VEL68_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36606.1"
                     /translation="HFSLEQNPYGWVQSALNRMTFPGGDLYSFLKSHSGRKRPENSPF
                     LAKMLLLSAALLLPSILISLLMAWRGEGGTLEAYFIKEG"
     gene            278..2368
                     /locus_tag="VEL68_11365"
     CDS             278..2368
                     /locus_tag="VEL68_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ36607.1"
                     /translation="MKDEVMPSLIPGEKKYPSWMTSVLLLALFLITLAVYYPSLRGDF
                     LNIDDQYYIDENEFIRDLSLKGIYNVFTSVIVVNYFPLQILSYAIDYHLWGLNPFGYR
                     LGNLIFHLGNTFLVFFFLRRLLAGRTLLSFAGSLIFSLHPVNVESVAWVSERKNVLSV
                     FFLFLSFLAYFRYLEGKRKGLDYGFSLGFYLLSILAKVSSVVFPLLLILYDICFTSRS
                     RKEWIKDKIPFFLMSAFFSGLAVYLYHLQKIIPEYHGGHPINNFLTMANVGVEYIFSL
                     FVPLYLNFYYDTRLVKSFGEYQFLLSLSFLLLVMVLAIKWYRKERPLFFSLLWFFIPL
                     LPVLNIVPIAILRADRYLYLPSVGIAYFLTWGIHRLLGNTGPKGSWAALGVLFLIAGI
                     FGWITEKQAGYWTSPFHLWSRVMKYIPSEAKAYCFLGNNARDRGEITLAETLYREALD
                     RAPRSPAALNNLGLLYLNKDRVEEAHALFQKALESDPDYPDAQINLGISYWKKGEEEQ
                     AIVAFEKAKGNKKNRATANNNLGVIYQNRGDYSRALALFREAVREGPQDMGACLNLAL
                     ALERNGQTDEAIVQLEKAHLRHPLGYPINYQLGSLYLKKGNPPRAAEYFSEALKLKPE
                     DPEVHFQLAKALNLIPGQEERSQFHLQKALREQRQDDRRMVFPPKRTEAGIRPGRGDK
                     NNHQSPESAERASP"
     gene            2665..3867
                     /locus_tag="VEL68_11370"
     CDS             2665..3867
                     /locus_tag="VEL68_11370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ36608.1"
                     /translation="MERKEVPASGFGLGSTRQEASSKNTFQDPKNRPGQDGFAAIPTT
                     DPEAALRFCQALSRFLLTTPFPPPSGRIPSFDDSVLRGQETAANPEISVVIPIFNEEE
                     NIPVLYSRLTDALKGTGMESEIIFVDDGSRDDSLKRLYSFRDEDPTVTIVELSRNFGH
                     QVAISAGLDHARGKAVMVMDADLQDPPELLPQFIKKWQEGHDVVYAIRKNRKEGWGKR
                     LAYSAFYHLLHRTANIEIPLDAGDFCIMDRKVVNLLMAMPERNRFVRGIRSWVGLDQV
                     GLTYERQARQAGQSRYTFLRLLYLALDGLISFSYLPLRVIAILGALISLFSLLMAIFY
                     AGEKLLVGLNPPGFATLTVAIFFFAGVQLVTMGVIGEYVGRIFEEVKQRPLYIVRRVT
                     RRPSKCVS"
     gene            3855..5048
                     /locus_tag="VEL68_11375"
     CDS             3855..5048
                     /locus_tag="VEL68_11375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ36609.1"
                     /translation="MRLLMLNNEFPPLGGGTGTVNLAILQRMASMPGLEVDLITSALG
                     KRAEEVDFAERVRIFKVPVNNRIIHHSTNRELIAYASRSWFLAGRLHRARAYDLCFAW
                     SAVPAGGVAWALRRTAGLPYLVRVGGPDIPGFEARYHLLYPVLTPMIRSIWRGAERVV
                     AKCAGEAEMIRAVDKDVPIALIPNGVELEAFPPASPPPESGPLRLLCVARLIERKGQR
                     LLIHAVKGLTDQGIDVLLDLIGTGDAQASYEAQARALGMEEKVRFVGYVPRERMAAHY
                     AAAHAFVLPSDNEGMSVATLEAMAAGLPLVVTRTGGTDDLVEEGVNGWTFPRGDEESL
                     MGHLRRLALDRSLARRLGEASRTRAAQFSWEQACDGYVHLFEKITCPARPRSSAGTCS
                     SNPGR"
     gene            5062..6960
                     /gene="asnB"
                     /locus_tag="VEL68_11380"
     CDS             5062..6960
                     /gene="asnB"
                     /locus_tag="VEL68_11380"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXZ36610.1"
                     /translation="MCGICGHLILNREKQVDRGLLERMNNSLIHRGPDSAGYHIRGNL
                     GLAMRRLAIIDVAGGHQPMKNEDGTLWVVCNGEIYNFRELRSDLEARGHRFATKSDVE
                     TILHGYEQWGDDALQHLNGMFALALWDEGKRRLLLARDRMGKKPLYWHLSPAGLLWGS
                     EAKALLPASWVERRVNALALHHYLTLQYTPDPLTIFEGMQQLPAGHKLVVEREGAPQV
                     FRWWQPSFEPKWKLTREEAMQQARELLGSAVERRLVSEVPLGAFLSGGLDSSLVVALM
                     AERSSRPVQTFSIGFEEKHYSETRYALQVARRYGTDHHEFLFRPLDLVQLIEEVLRSA
                     DEPLADPAALPLYELARQARAFVTVALCGDGGDETLAGYRRYVLDGLLRPYAALPKWV
                     TQSLVPSAASLLPEPAWLPEDRNPFTGLKRLGQFSAVSPKASLVRWGSYFTHGDKLGL
                     YTDRWREELTRQDTADWVALAFDEAPAGNLLDRTLYADQMTYLAGDLLPKTDRMTMAH
                     ALEARAPFLDRDWVEWTARLPVKYKVRGWKTKWLLQAAFADKLPPEILHRGKQGFGVP
                     LGLWLRGPLESWARARILENRALREWFRLEAMHRLFEENRSGRVNHGKRLWSLLMFSL
                     WLDQHFKR"
     gene            6966..8483
                     /locus_tag="VEL68_11385"
     CDS             6966..8483
                     /locus_tag="VEL68_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXZ36611.1"
                     /translation="MRWFDFGSNWQAFSARRLDAGRLTAAVHSLQALLGKETLGGLSF
                     LDVGCGTGLFSIAAHQLGAVKVVGIDLNPSCISISEQNRDRLAPKAPVLFQLGSAINR
                     EDLHSLGRFDIVYAWGSLHHTGAMWAAIRNVAQQVSPGGLLVLAIYNKHFTSPVWKLI
                     KWFYNHLPGWGRVLMAVLFSGIIYIAKFLVTGRNPLEKERGMDFWFDVVDWVGGYPYE
                     YTTPLEVEKFVKAQGFNTRHRIPARVPTGCNEFVFVKNDGPYFGNDLRDFMPTMKSGE
                     YDQGWEMKWDDMKKYGPFSRHLRRIIKNLIRPLAFHSVLDVGCGQGSLLWELQAQFPH
                     MKPTGTDLSMKALDLARRRVPEGEFHVSDLAQESLEDKFDLVICSEVLEHIPDDQTAI
                     RHLARMTGKYLVVSGPQGRMRRFEATVGHVRNYAYGELVHKLEQNGFRMVRVIEWGFP
                     FYSPLYRNFLDLLGGRGTEGQFGPARRLTSIGLYHLFRLNSAKRGDELIVLAEIA"
     gene            8608..9411
                     /locus_tag="VEL68_11390"
     CDS             8608..9411
                     /locus_tag="VEL68_11390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HXZ36612.1"
                     /translation="MKRVKLFSPDFTPGKVLVTAKAIADIIILAVQKPSKKTLHFARM
                     ILKVKPRFTMVKNKNLINLYHLVQEADRLNLPGDIVECGVWNGGSAAFMAVAHAEGPN
                     PRLRTLWLFDSFEGLPRPDERDGEEERKAYFEEWNKGDIEKVKQVFKKMGLGLEKVKI
                     VPGWFEQTLTTTPVGPIALLHIDADWYSSVKMALEAFYDKVVPEGFVILDDYGYWKGC
                     TQALQDFLFEHERAQVRIEPMGRDGGYFQKPVTDPKKRPGIPTKEMGRQ"
     gene            9510..10214
                     /locus_tag="VEL68_11395"
     CDS             9510..10214
                     /locus_tag="VEL68_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ36613.1"
                     /translation="MGPFLGSPRSLTVLDLANGRLQPQFLLLKASGHRVCGVDLVNRP
                     GKDWVDWAYRGARLLYRWKLGLPATKAVDPGLVCGRVNTLPFQSNRFDLVTSIAAFEH
                     FSDVPAVVTELHRVLRLSGLAWILIHLFSSPSGGHNLGWTETPRRDVPKGVDPWDHLR
                     KRRLPFTVPLNEWRLGQYLTAFSRHFEILKSYCAIREGEGWLTPEIMAELSDYSREEL
                     TCGAYVIVARKQQGRE"
     gene            10216..11781
                     /locus_tag="VEL68_11400"
     CDS             10216..11781
                     /locus_tag="VEL68_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36614.1"
                     /translation="MTEPKVTPIPMRNRIYWHAAAIVCLGALLFLAGSIKLESFPPLW
                     WDEGWTLTVARNWVERGHYGRLLAGEPTPSGLEAAFPVTALVGLSFHLLGVGVWQGRM
                     MGLVFMVGALGMMYYLALRLHDQRVAIGTLGVILLMSPHSAIHPLYMGRQVLAEIPSV
                     FYLLGGYAFFWLALNRSAGFLSVAVVLWGVGILSKITVLPFWFISLSLPLGVTLIRRR
                     WKLASLFGAGLLGSPCAAWMLGRGGQLLLPGHRTLTQPLQGAYEVTALVFEGFNRLFA
                     LQIILQFALPTLIALCFAAGKLFQKREQAELTSEVELMRLALLGLSGSWFIWFALLSI
                     GWPRYLLPPMFISSLFVAVLLNDLTASFHLPSLKATLIEAVKNRSFNQKIGGFLLVTI
                     LIFMTLPPTLKLLYHQYSARGDTSAMETAKFLNSQTPAHALVETYESELHFLLQRKVH
                     YPPDQVHVELNRRTFLGQDVIVGYDPLAADPDYLVIGSQGRMWQLYDPVVASGAFRLI
                     NRIGWYQVYERAR"
     gene            complement(11907..>13529)
                     /locus_tag="VEL68_11405"
     CDS             complement(11907..>13529)
                     /locus_tag="VEL68_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ36615.1"
                     /translation="FSKYPIFAERISQGVDLGERILDLSPLYLYLNLLFQKVYGRSWE
                     GLALLQVFLGSLNVLLIYLIGEKVFNRTVGFMGALLLILYGNMTLFELTLEPEALLIF
                     LNSLGVLSLLIAQERILSGSSPWGFFLPGILLGLSVITKPNGLLFLPPAILWIGRTQV
                     DGFIRMKASLFLLLGVFLVVSPITLRNYLNFHDFVLITADGGKVFYHGNGPGSTGMER
                     ADLPDQGFREEGMGDPDPAHALFRQAARSISGKPLKPSECSGFWFRRTLDFMSSRPLT
                     AFSLVGKKFFYFWGNYEVHEIDSNYKYDLILRRWPLVPFGVLSILGMVGMVLARKGFR
                     QAFLLYSMVGVYLLSVLIFFAASRYRLPAAPFLALFGAFALTSFYTLWKEKRRIALSA
                     LLSISLLLWPLINLPFRSEVKALERWQMATRIHYSLGGNFLFQKRQYREAIEEYRKAL
                     ALAPDFAPAYNRLGMCHAILNELDEAEEDFQKVIELAPNMDQGYLNLGMVYLLKGKPG
                     IAIPLLEKALSLNPENTRAREQLQNLRSRSPG"
BASE COUNT         2843 a         3668 c         3959 g         3059 t
ORIGIN      
        1 ccatttttcc ctggagcaaa acccttacgg gtgggtccag agcgccctta atcggatgac
       61 ctttcccgga ggggacctgt attctttcct gaaaagtcat tccgggagga agaggccgga
      121 gaactcgccc tttttggcca agatgctcct attatccgct gctctcctcc ttccctccat
      181 cctcatatcc ttgctcatgg cctggagggg ggagggtggg accctggaag cttactttat
      241 caaagaagga tgaggctgga tgcggatgaa ttggatcatg aaagatgagg ttatgcccag
      301 cctgatcccg ggagaaaaga aatatccttc ctggatgacc tcggtcctcc tgctcgctct
      361 attcctgata accctggcgg tttactaccc ttccctccgg ggcgacttcc tgaacatcga
      421 cgaccagtac tatatcgacg agaatgagtt catccgcgac ctgagcttaa agggaatcta
      481 caatgtcttc acctcggtca tcgtggtgaa ttacttcccc ctccagattc tctcctatgc
      541 catcgattat cacctctggg ggttgaaccc cttcggctat cggctgggaa atctcatctt
      601 ccacctgggt aataccttcc tggtcttctt ttttctgcgc cgcctattgg cagggagaac
      661 cttgctttcg tttgcgggaa gcctgatctt ttccctccat ccagtgaacg tggagtccgt
      721 ggcctgggtc tcggagcgca agaacgtcct ctcggtattc ttcctcttcc tctcgttcct
      781 agcttacttc cggtacctgg agggaaagag gaaaggcctc gactacgggt tctctcttgg
      841 gttttatctt ctctccatcc tggccaaggt ctcctcggtg gttttccctc tgctcctcat
      901 cctgtacgac atatgcttca ccagccgttc ccggaaggag tggatcaagg acaagattcc
      961 tttcttcctc atgagcgctt ttttctctgg gttggccgtt tacctctacc acctgcagaa
     1021 aattatccca gagtaccacg gtggacatcc gatcaataat ttcctgacca tggccaacgt
     1081 aggggtggaa tacattttct ccctcttcgt acctttatac ctcaacttct actatgatac
     1141 ccggctggtt aaatcctttg gggagtatca attcctcctc tctctatctt tccttctgct
     1201 ggttatggtt ctggccatta agtggtaccg caaggagcgg cccctattct tttccctgct
     1261 ctggttcttc attcctctat tgccggttct taatatcgta cccatcgcca tcctgcgagc
     1321 cgaccgctac ctctatcttc cctcggttgg tatcgcctat tttctgacct ggggaataca
     1381 ccgcctcctg ggcaatactg ggccaaaagg ttcctgggct gccttgggag ttctcttctt
     1441 gatcgccggg atcttcgggt ggatcacgga gaagcaggct ggctattgga cgagtccttt
     1501 tcatctctgg tctcgggtga tgaaatacat ccccagcgaa gcaaaggctt attgcttcct
     1561 gggaaacaat gcccgcgacc ggggggaaat cactcttgcg gagacccttt atcgagaagc
     1621 cctcgaccgc gcgccacgct ccccggcggc cttgaataac ctgggactct tgtatttgaa
     1681 caaagaccgg gttgaagagg cccatgccct ctttcaaaag gccctggaga gcgaccccga
     1741 ttatcccgat gcccaaatca acctgggaat cagctattgg aagaaggggg aggaagaaca
     1801 ggccattgtc gcttttgaga aagccaaggg aaacaagaaa aaccgggcga ccgcgaataa
     1861 caatctggga gtcatatacc agaatcgagg agattattcc cgggccttgg cccttttccg
     1921 ggaagcggtg cgtgagggtc cccaggatat gggtgcctgt ttgaacttgg ccctggccct
     1981 ggaaagaaac gggcagacgg atgaagccat tgtccaattg gagaaggccc acctccgcca
     2041 tcccctcggg taccccatca actaccagtt gggaagcctc tacctgaaaa aaggcaatcc
     2101 tccccgggcg gcagaatatt tctccgaagc gctcaaactg aaacccgaag acccggaggt
     2161 gcatttccag ttggcgaagg cattaaacct cattcccggg caggaagaaa ggagccagtt
     2221 ccatttgcag aaagctctgc gcgagcaacg acaagacgac cggaggatgg tgttcccccc
     2281 aaaacggacc gaggcaggaa tccggccagg aaggggagac aaaaacaatc atcagtcccc
     2341 ggaatcagca gaaagggctt ccccgtgatc tctccaccct ggttatgggc tatattagcg
     2401 gcctcaatct tttcgatggg aatcggtata tggggggcat accgctggag gaaaccttgg
     2461 gacggagcag gggccctgtg ggccttgctg ggattggctg gaggggtgat cgccactctc
     2521 ctcctgcatg ttcccagttt tttccgttcg ccctgaagaa ggattcgttg accagaacct
     2581 ttaacccgtg ggcagaaaga atttaggaaa gcccgttcaa cgaccgaaga ttcgcacccc
     2641 atgggtaaga agccaggaga ggaaatggaa agaaaagaag ttccggcatc gggttttggg
     2701 ttgggttcaa cccggcaaga agcctcctcg aagaatacct tccaggaccc caaaaatcgg
     2761 ccgggccagg acggtttcgc agccattcca accaccgatc cggaagccgc cctccgtttc
     2821 tgccaggctc tctcccggtt tctattgacg acacccttcc ctccccccag cgggagaatc
     2881 ccttctttcg atgattccgt gcttcgcggg caggagaccg ccgcaaatcc agaaatttcc
     2941 gtggtgatcc ccatcttcaa tgaagaggag aatattcccg ttttgtattc ccgcctcacc
     3001 gacgcgctaa aagggacggg aatggaatcg gagattattt ttgtggacga tggcagccgg
     3061 gatgactcct tgaaacggtt gtattccttc cgggatgaag acccaacggt gacgatcgtt
     3121 gagctttccc gcaatttcgg ccaccaggtg gcgatcagcg ctggcctgga tcatgctcgc
     3181 gggaaagcgg tcatggtgat ggacgcggat ttacaggacc ccccggagct gttgccgcaa
     3241 ttcatcaaaa agtggcagga aggtcatgac gtagtctacg cgatccgaaa aaaccgcaag
     3301 gaagggtggg ggaaacgcct ggcttattcc gccttttatc acctcttgca tcggaccgcg
     3361 aatattgaga tccccttgga tgcaggggat ttctgcatca tggatcgaaa ggtcgtgaac
     3421 ctgctcatgg ccatgcccga gcgcaaccgc ttcgtgcgag gaatccggag ctgggtggga
     3481 ttggatcaag tcgggctgac ctacgaacgg caggcccgcc aggccggcca atccaggtac
     3541 acgttcctgc gactacttta cctggccctg gatggattga tttctttcag ctatctgccc
     3601 ttaagggtga tcgccatcct gggggctctg atctcccttt tttcgcttct catggccatc
     3661 ttctacgcgg gggagaaact actggttggc ctcaatcccc ccggattcgc caccctaact
     3721 gtggccatct ttttcttcgc cggcgttcaa ctggtgacca tgggggtcat cggtgaatac
     3781 gtgggccgga tcttcgagga agtgaagcag cggccgttgt atattgtgcg gcgggtgacg
     3841 cggagaccgt ccaagtgcgt ctcttgatgc taaacaacga atttccgccc ttgggaggcg
     3901 gaaccgggac cgtcaacctg gcgatcctcc agcgcatggc cagcatgccg gggctggagg
     3961 tggacctgat cacttcggcc ctgggaaagc gggccgagga agtcgatttt gccgaacggg
     4021 tccggatctt caaggttccg gttaataacc ggataatcca ccattccacg aaccgcgaac
     4081 tcatcgccta tgcctctcgg tcctggttcc tcgccgggcg gttgcaccgg gcccgcgctt
     4141 acgatctttg cttcgcgtgg agcgcggtgc cggccggcgg ggtagcgtgg gcccttcgcc
     4201 gaacggccgg gcttccctac ctggtgcggg tcggcgggcc ggatattccg gggtttgaag
     4261 cgcgctatca tctcctgtac ccggtgctta cgccgatgat tcgctccatc tggcggggag
     4321 ccgaaagggt ggtggcgaaa tgcgcggggg aagccgagat gatccgggcg gtggataaag
     4381 acgtccccat cgccctcatc cccaacggag tagagctgga ggcgtttccg ccggcgtccc
     4441 ctcctccgga aagcggcccg ctgcggctgt tatgcgtggc ccgcctgatc gagcgcaaag
     4501 gacagcgcct tttaatccac gccgtcaagg ggctcaccga ccaggggatc gatgtattgc
     4561 ttgacctgat cggcacggga gatgcccagg cctcctatga ggcccaggcg agggctctgg
     4621 ggatggagga aaaagtccgc ttcgtcggtt acgtgccccg cgaaaggatg gcggcccatt
     4681 atgcggcggc ccacgccttc gtgcttcctt ccgataacga ggggatgagc gtggctacgc
     4741 tggaagccat ggccgccggg cttcccctgg tggtgacccg gaccggcggg acagatgatc
     4801 tggtcgaaga aggggtgaac ggctggacct tcccccgggg ggatgaggag agcctgatgg
     4861 gtcacctaag acggctggcc cttgaccgtt ccctggcccg ccgcctggga gaagcctccc
     4921 gcacccgggc ggctcaattc tcctgggaac aggcctgcga cgggtatgtg cacctttttg
     4981 aaaagataac ctgtccagcc cggccccgga gttcggcggg gacctgttct tcaaacccgg
     5041 gaaggtgaag aagatcgtac catgtgcgga atctgcggcc atctcatctt gaatcgggag
     5101 aagcaagtgg accggggact tctcgaaagg atgaataatt cgctgatcca tcgcgggccg
     5161 gacagcgccg gatatcatat ccggggaaac ctgggcctgg ccatgcgccg gctggcgatc
     5221 atcgacgtgg ccggcggaca tcaacctatg aagaatgaag acggaaccct ctgggtcgtc
     5281 tgcaacgggg agatctacaa cttccgggag ctgcgttccg acctggaggc gcggggacac
     5341 cgctttgcca cgaagagcga tgtcgaaacc attctccatg gctacgaaca atggggcgat
     5401 gatgctctgc agcacctgaa cggcatgttc gccctcgcgc tctgggacga ggggaagagg
     5461 cggcttttgc tagccaggga ccggatgggg aagaagcctc tctactggca tctttccccc
     5521 gcggggctgc tgtgggggtc ggaagcgaaa gccctgctcc ccgcctcctg ggtggagagg
     5581 cgggtgaatg cgctagccct gcaccattac ttaaccctcc agtatacgcc cgacccgctc
     5641 acgatcttcg aagggatgca gcaacttccc gcggggcaca agttggtcgt ggagcgggag
     5701 ggggctcccc aggtcttccg ctggtggcaa cccagcttcg aaccgaaatg gaagctgacc
     5761 cgggaagagg cgatgcagca ggcccgcgag ctgctcgggt cggcggtcga gcggcggttg
     5821 gtttccgaag tgcctctcgg ggctttcctc agcggcggat tggactcttc cctggtggtc
     5881 gcgctgatgg ccgaaagatc ttcccggcct gtccagacct tttcgatcgg attcgaggaa
     5941 aaacactact cggagacgcg ctacgccttg caagtggccc ggcggtatgg aaccgaccat
     6001 catgaattcc tcttccgccc gctggacttg gtccagctga tcgaggaagt gctccggtcg
     6061 gccgacgagc ccctggccga cccagccgcc cttccgctgt acgaattggc caggcaggcg
     6121 cgcgccttcg tcaccgtggc cctgtgcggg gacgggggag acgagacgct ggccggctac
     6181 cggcgctatg tgctcgacgg tcttttgcgg ccttacgccg cgctgccgaa atgggtgacc
     6241 cagtccttgg tcccttccgc cgcgtccctt ttgcccgaac cggcctggct cccggaagat
     6301 cggaacccct tcaccggact gaagcggctc ggccagttct ctgcggtttc gcccaaggcg
     6361 tcgctggtcc ggtgggggtc ctatttcacc catggggata agctcggcct ttacaccgac
     6421 cggtggaggg aggagctgac ccggcaagac acggccgatt gggtcgcctt agccttcgac
     6481 gaggcgccgg ccgggaacct gctggaccgc actctctacg ccgaccagat gacctacctc
     6541 gcaggagacc tgctgcccaa gaccgatcgc atgaccatgg cccatgccct ggaggcccgg
     6601 gccccttttc tggatcgcga ctgggtcgag tggaccgccc gcctcccggt gaaatacaaa
     6661 gtgcgcggct ggaagaccaa atggcttctc caggcggcct ttgccgataa attgcccccg
     6721 gagatcctcc accgggggaa acagggattt ggcgttcccc tggggctgtg gctgcggggg
     6781 ccgctggagt cctgggcccg ggcccgaatt ctggagaatc gggccctccg ggaatggttt
     6841 cggctggagg cgatgcaccg gttgttcgag gaaaacagaa gcggccgggt caatcacggc
     6901 aagcgtttgt ggagtctgtt gatgttttct ctctggctgg atcaacattt caaaaggtga
     6961 gcccaatgcg ctggttcgat tttggctcca actggcaagc tttttcggcc agacggctcg
     7021 acgccgggag gctcacggct gccgttcatt cccttcaggc tctcctggga aaagaaactt
     7081 taggaggtct aagtttcctc gatgtcggct gtggaacggg tcttttttcc attgccgctc
     7141 atcaattggg cgcggtcaag gtggtgggaa tagacctgaa cccgtcctgc atctccattt
     7201 ctgagcagaa tcgggaccgt ttggcgccaa aagcgccagt cctattccag ctggggtccg
     7261 ccataaaccg ggaagacctg cattccctcg gaagattcga tatcgtttac gcgtggggct
     7321 cgctgcacca tacgggcgcc atgtgggcgg ccattcgtaa cgtggctcaa caggtttcgc
     7381 cggggggcct gctggtgttg gccatctaca acaagcactt tacctcaccg gtatggaagc
     7441 taatcaaatg gttttacaac caccttcccg gatggggaag agttctgatg gcggtattat
     7501 tctcaggaat catttatatt gccaaatttc tcgtcacggg acggaaccct ctcgaaaaag
     7561 agcgaggaat ggatttctgg ttcgatgtgg ttgactgggt gggaggatat ccttatgaat
     7621 ataccacccc tctggaagta gagaaattcg ttaaggctca agggttcaac acccgtcatc
     7681 gcattcctgc gagggttccc acaggatgca atgaatttgt cttcgtcaaa aatgacggcc
     7741 cgtattttgg gaatgatctg agggacttca tgccaacgat gaaatcgggc gaatatgatc
     7801 aaggttggga aatgaagtgg gacgatatga agaagtatgg tcctttttct cggcacctgc
     7861 ggcgaatcat caaaaatctt atccgcccgc tggcatttca tagcgtcctg gatgtggggt
     7921 gcggccaggg atctctcctg tgggagttgc aggcccaatt tccccacatg aaacccacgg
     7981 ggacggatct gtcgatgaaa gcgctcgacc tggcgcgtag aagggtaccc gagggggagt
     8041 tccatgtgag cgacctggcc caggagtcgc tagaggacaa gttcgacctg gtcatctgca
     8101 gcgaagtcct ggaacatatt cccgatgacc agaccgccat ccggcatcta gcccgaatga
     8161 cgggaaaata ccttgtcgtg tccgggcccc aggggcgcat gcggcgtttc gaagcgactg
     8221 tgggccatgt gcggaattac gcttacggcg agttggttca taaactcgaa cagaatggct
     8281 tccggatggt tcgggtcatt gaatggggtt ttccgtttta ttcgcccctc taccggaatt
     8341 tcctggatct tctgggcggc cggggcacag agggccagtt tggccccgcg cgcagattga
     8401 cttccatcgg cctgtatcat ctgttcaggc tcaattcagc gaagcgtggc gatgagctta
     8461 tagttctagc ggagattgcc taagaccttt cggttctccg ggttgttccc cccctagggg
     8521 ggcgagggta gggtaagacc cattgtctgg gggaatgaaa ttggcgcggg tccatccccg
     8581 gtaaaattcc ttttaggtcc cattcctatg aagcgagtca agcttttttc ccctgacttt
     8641 accccaggca aagttttggt taccgcgaag gcaatcgccg atattatcat tctggcggta
     8701 cagaaaccgt cgaagaaaac cctccacttt gccagaatga tcttgaaagt gaaaccgaga
     8761 ttcacgatgg taaagaacaa gaacctgatc aacctgtatc atttggttca agaggccgac
     8821 cgattaaatt tacccgggga tattgtggag tgcggcgtct ggaacggtgg gtctgccgcc
     8881 ttcatggcgg ttgcacacgc agaggggccc aatcccaggt tgagaactct atggctgttt
     8941 gactcttttg aaggactccc tcgtcccgat gaacgagatg gagaggagga aaggaaggcg
     9001 tattttgagg agtggaacaa aggagatatc gaaaaggtaa aacaggtatt caagaaaatg
     9061 ggtttgggat tagaaaaagt caagatcgtt cccggatggt ttgaacaaac gttgaccaca
     9121 actcccgtgg gacccatcgc cctcttgcac attgacgccg actggtacag ctcggtgaag
     9181 atggcattgg aagcattcta tgacaaagta gtcccggaag gatttgtcat tcttgatgat
     9241 tatggatact ggaagggatg tacccaggcc ctgcaggatt ttctcttcga gcacgaaagg
     9301 gctcaagtac ggatcgagcc gatgggtcga gacggggggt attttcaaaa gcccgtcacc
     9361 gacccgaaga agcggccggg aatcccgacg aaagaaatgg gtcgtcaatg aacccctcgg
     9421 taaccgatct tttccggtat gcgtggcaaa tgctgagcgg gtttcgcgtg aacctcgaat
     9481 tgtccctcgc gaatgcgcgc tggaaggata tgggaccatt cctgggttcg ccccgatcct
     9541 tgacggtgct tgatttagcc aacggccggt tgcagcccca gtttctcctc ttgaaggctt
     9601 cgggccatcg ggtgtgcgga gtggacctgg tcaatcgccc gggaaaggac tgggtggatt
     9661 gggcctatcg tggggcgcgc cttttataca ggtggaagtt aggattaccg gctacgaaag
     9721 ctgttgatcc ggggctcgtt tgtggccggg tgaatacctt accctttcaa agtaatcgct
     9781 ttgacctggt tacctccatt gcggctttcg aacacttttc ggatgtcccg gcagtggtga
     9841 ccgaacttca tcgagtgctt cgattgtcgg gcttggcatg gatattaatc cacctctttt
     9901 cgagcccttc aggtggacat aacctcggct ggaccgagac ccccagacgg gacgtcccga
     9961 aaggggtgga tccgtgggac cacctgagga agcgccggct tccatttacc gtacccctga
    10021 atgagtggcg attggggcaa tacctgaccg ctttctcccg ccacttcgaa attctcaaga
    10081 gctattgtgc gatacgggag ggagaaggat ggttaactcc cgaaattatg gctgaattgt
    10141 ccgattatag ccgggaggaa ctaacctgcg gggcttatgt catcgtggcc cggaaacagc
    10201 aagggcggga atgaaatgac ggagccgaaa gtcaccccaa ttcccatgag gaatagaatt
    10261 tattggcatg cggccgcaat tgtctgcctg ggggccttgc tctttttggc cgggtcgatc
    10321 aaattggaga gctttccccc cctttggtgg gatgaaggat ggactctaac ggtggcccga
    10381 aactgggtgg aacgaggcca ttacggccgt ttattggccg gagagcccac gccatccgga
    10441 cttgaagccg ccttcccggt taccgccctg gtaggactca gtttccacct cttgggtgtg
    10501 ggggtctggc aagggcggat gatgggctta gtctttatgg tgggggcgct ggggatgatg
    10561 tactatctgg ctcttcgttt gcatgatcag cgcgtggcca tcggcactct gggggtcatt
    10621 ctattgatgt ccccccactc agccattcat cccttgtaca tgggccggca ggttttggcg
    10681 gaaatcccgt cggtgtttta cctccttggg gggtatgcct ttttctggtt ggccttgaat
    10741 cggtcggcgg gctttttatc ggtggcggtt gtcctttggg gcgttggcat cctatccaag
    10801 ataacggtgc ttcctttttg gtttatttcc ttgtctttgc ctttgggcgt cactctgatc
    10861 cgacgcaggt ggaagctggc cagtctgttt ggggcagggc tcttaggctc tccttgtgct
    10921 gcgtggatgc tggggcgggg gggccaactg ctcttgccag gtcataggac cttgacccaa
    10981 cctcttcaag gagcttacga agtcacggcc ctggtattcg aggggtttaa ccgtttattc
    11041 gccttgcaga taatcctcca atttgctttg ccgaccttga ttgccctttg ttttgcagcg
    11101 ggaaaacttt tccagaagag ggagcaagct gagctaacct ctgaggtaga actcatgcgc
    11161 ttggcgctgc tagggctatc cgggagttgg tttatttggt ttgccttgct ttccataggc
    11221 tggcctcgtt atttgttgcc gcccatgttt atcagtagcc tgtttgtagc cgtcttgctc
    11281 aacgacctga ctgcttcttt tcatctgccc tccctaaaag ccactcttat cgaggccgtt
    11341 aaaaaccgga gcttcaatca aaaaatcggt ggattcctgc tggtaacgat tctcatcttc
    11401 atgaccctgc cgccgacttt gaagttgctc tatcatcagt attccgcccg gggcgacacc
    11461 tcggccatgg agacggcgaa attcttgaat tcccaaaccc cggctcatgc cctggttgag
    11521 acctacgaaa gtgaacttca ctttctgctg caacggaagg ttcattatcc tccagaccag
    11581 gttcatgtgg agctgaaccg acggaccttc ctggggcaag acgttattgt tgggtatgac
    11641 cctctggccg cggacccgga ctatctagtg atcgggtcgc aagggcggat gtggcaactt
    11701 tacgatccgg ttgtggcctc gggagccttt cgcctgatca atcgaattgg ttggtaccag
    11761 gtgtatgagc gagcgcgctg aatttaggta cgagtccccc ttgccttttt tgcccggagg
    11821 gcgaaggaga gggagctacc gaacgatggg agtcggataa aagaccttag ggtgggaagg
    11881 aaagacgaaa ctcgaatgag gagcgatcat ccgggggatc gggagcggag attttgcagc
    11941 tgctctcggg cccgggtatt ttccggattc agggaaaggg ccttctccaa gagaggaatc
    12001 gctattcccg gtttgccctt caacagatac accattccca ggttcagata gccctggtcc
    12061 atgttcgggg ccagctctat gaccttctgg aaatcttcct cagcctcatc cagctcgttc
    12121 aggatcgcgt gacacatccc cagccgattg taggccgggg cgaaatcggg agcgagagcc
    12181 agcgccttgc ggtattcttc gatggcctcg cggtactgtc gtttttgaaa aaggaagttt
    12241 ccccccaggc tgtaatgaat ccgggtggcc atttgccacc gctcaagggc cttaacctca
    12301 ctccggaaag gcaaattgat caaaggccag agaaggagag aaatgctaag gagagcggaa
    12361 agggcgattc tccttttttc cttccagagg gtgtaaaagg aagtcagagc aaaagcacca
    12421 aagagggcca gaaaaggggc tgcgggcagc cggtagcggg aggcggcgaa aaagatcagg
    12481 accgaaagga gatagactcc caccatggag taaaggagaa aagcctggcg aaatcctttc
    12541 cgggccagca ccattcccac cattcccagg atggagagga ccccaaaagg aacgaggggc
    12601 cagcgccgca ggatgaggtc gtacttgtag ttggagtcga tctcgtggac ttcgtagttt
    12661 ccccagaagt agaagaactt ctttcctacc agggaaaatg cggtcagagg acggctgctc
    12721 ataaagtcca gagtccgacg aaaccagaaa ccggagcact ccgagggttt taaaggtttt
    12781 cccgaaatgg aacgggcggc ttgccggaaa agggcatggg ccgggtccgg gtcgcccatc
    12841 ccttcttccc tgaaaccctg gtcgggcaaa tcagcccgtt ccatccccgt gcttccgggg
    12901 ccgttgccat gataaaagac ctttcctccg tcggcggtga tcaagacgaa atcatggaaa
    12961 ttcaaatagt tgcgcaacgt gatgggagaa acaacaagga aaacccccaa gaggaggaaa
    13021 agggaagctt tcattcggat gaatccatcg acctgcgtcc gaccaatcca gaggatggcc
    13081 ggcggtagga agagaagacc attgggcttg gtgatcaccg acaacccgag aagaattcca
    13141 ggaaggaaga atccccaggg cgaagagccg gaaaggattc tctcctgggc gatgaggaga
    13201 gacaagaccc ccagggagtt gaggaagata agcagcgctt ccggctccag agtcagttcg
    13261 aagagggtca tattcccgta gaggatcagg agaagggctc ccataaatcc cacggttcga
    13321 ttgaacactt tctccccgat gaggtagata agaaggacat tcaggcttcc caagaagacc
    13381 tggagaaggg ccagaccttc ccagcttctt ccgtagacct tctgaaaaag caggttgaga
    13441 tagagataga gggggctcag gtcgaggatg cgttccccga gatcgacgcc ttggcttatc
    13501 cgttcggcga aaatgggata cttgctgaa
//
LOCUS       DASPRR010000300         6015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_553461, whole genome shotgun sequence.
ACCESSION   DASPRR010000300 DASPRR010000000
VERSION     DASPRR010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553461"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VEL68_11410"
     CDS             complement(<1..860)
                     /locus_tag="VEL68_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ36616.1"
                     /translation="MDILGNILYGFSITLTWTNLFYCFAGAFIGTLVGVLPGLGPVAA
                     MSLLFPATLHIPPVSAIIMLAGIYYGAMYGGSTTSILVNIPGEAASVVTCIDGYQMAR
                     RGRAGVALGISALGSFIGGTLAVVALQLVAPPLAKIALAFGFPEYFSLMCCGLVVLTF
                     MARGSMVKALMMAVVGIFLGTVGTDLIMGTPRFTFGIQIFMDGLGVVPVIMGLFGISE
                     VILNIEEGVAREVFKDKIDHLLPNLKDWADSIGPILRGSLIGFFLGILPGAGPVVSSF
                     TSYATEKKLS"
     gene            complement(870..1328)
                     /locus_tag="VEL68_11415"
     CDS             complement(870..1328)
                     /locus_tag="VEL68_11415"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ36617.1"
                     /translation="MNRESWGSVAWVFLGVLICLGSVKLSLGNFRNPGPGFLPFITGA
                     ILTGLALVVFLQSRRTGQGNKERKPFLADKTKGLKAVLTLIGLLAYAVGMEYLGFLLS
                     TTFFLAFLLGVVEPQKWYIVVLGSILSSVASYTLFEILLKSPLPKGFFEF"
     gene            complement(1411..2385)
                     /locus_tag="VEL68_11420"
     CDS             complement(1411..2385)
                     /locus_tag="VEL68_11420"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36618.1"
                     /translation="MNKRRAVLIILAVFFLIFSAAGTPWAAEKKFPVKPIQVIIPFQP
                     GDTDNLLRPFLEKMPEFLGQPVNMVYKPGAAGSVGAGFVAASKPDGYLLVGSSHSSIV
                     VVPLTHKDVGYTWKSFAPVCAMVEASSLLVVQSSSPWKTLKDLIEDSKQSPGKVTYSS
                     SGTFGSNHMIPEAMCKEAGIKWTYIPSQGSGPTITALLGGHVQVASTAIAPALPHLRA
                     GALRALAVFKPTRMKALPDAPTLRELGFSIHSASPYGILAPKDTPKEVVEAIYLAAKK
                     AADKYGDSISDRLDKIGAEIGLEGPEEYTAHLGRQYELFSGIYRIVKQ"
     gene            complement(2491..3903)
                     /locus_tag="VEL68_11425"
     CDS             complement(2491..3903)
                     /locus_tag="VEL68_11425"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ36619.1"
                     /translation="MNFSNSKLDPLLHPKSIAVLGASQDTTKLVGKPIAFLLQHKYGG
                     KIYPVNPKYKEIAGLTCYSSLAQVPGEIDAVVVGLPAESVLDAVKECAERKVHSVIVF
                     ASGFAEIGGKGEEMETELRDLARRSGLILCGPNCQGVVNLHEGATLSFTPALERKQLL
                     AGKIAFVTQSGAMGGSLFSNAMQLGIGFSYWISSGNEAALESADYIRYLVEDPKTSVI
                     LNYSEGYRDEAKFIQAAEAARRAGKPIVALRVGRTPMGKRAAQAHTGAVCGPDPEVEA
                     LFQRLRILRVQDGDELLEIGNVLVQGRLPKGRSVGILSTSGGAGGLMADQCEMGELEV
                     PELQGATREEYVRMLPYFGSALNPVDTTAMFSQFLAKDREYFKKLLRPMVREKSIHSI
                     ILMITMSTGERAKKMAQDIVEIYQETDKPMVISWIAGNLAEEGYQIIRGAGIPLFMNT
                     KKSVQALRTLVRYAEMREKT"
     gene            complement(4094..4894)
                     /locus_tag="VEL68_11430"
     CDS             complement(4094..4894)
                     /locus_tag="VEL68_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXZ36620.1"
                     /translation="MPFETLLLEKKEGIGLLTMNRPDRMNALNVQMEAELVQALQELD
                     QDDEVRVLVVTGAGKAFCAGADVGRMSGEERERKPRGAEEFRRGFRNIQGVILGLQRL
                     EKPTIAMVNGAAVGGGFDLACACDLRVGSENARFMVAFTRIGLFPGWGGTWLYPRIIG
                     IPKAAEMLFTGDFMEAKEAERLGLLNKLVPASELAKETMDLARRIANGPPIAIRLAKL
                     QIYKGLEMDLETAMKVAAACETITLTSEDHKEGVAAFREKRKPQYKGR"
     gene            complement(4988..5971)
                     /locus_tag="VEL68_11435"
     CDS             complement(4988..5971)
                     /locus_tag="VEL68_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ36621.1"
                     /translation="MSRYFIRVIVGLAIMGSVSLGLAAGEAAAQRKYLSLGTGNPGGT
                     FYFIGAGFANLFNRYVPGVRVIAESTAASEENFHYVIRKKMDLGLISIHVIAPALEKK
                     MDLSGVRLVSLGHTSDRHWFVRKDSPIKSISDFRGKRVALGSPGSGTLVSSKMELGAA
                     GKLGLEDFKPAYLSFTESITALKDGTVDVGVISAGYPVASLLDLARTVPIRLIPYSEQ
                     EMKAIMDAYPFYVKVVIPAGTYQGIDTDTLTRGIVTAIFCRQEMSEDLVYQMMKALYD
                     HPKEKDAIHPQARQWSLENMYRGAEYTTKYIPFHPGAVKYLKEKGIWKETR"
BASE COUNT         1316 a         1738 c         1645 g         1316 t
ORIGIN      
        1 ttggacagct ttttctcggt ggcataggaa gtgaaactgg aaaccaccgg cccggccccg
       61 ggtaagattc ccaggaagaa gccgatcaag ctgccccgca ggatggggcc gatcgaatcg
      121 gcccaatcct tcaggttggg gaggaggtga tcgatcttgt ctttgaagac ctcccgggca
      181 actccttcct caatgtttag gattacttcc gaaatgccga acaggcccat gatcaccggg
      241 accaccccca gcccgtccat gaagatttga atcccaaagg tgaagcgagg agtccccatg
      301 atcaggtccg tacccacggt gcccagaaaa atccccacca cggccatcat caaagccttg
      361 accatagaac ccctggccat gaaggtgagg accaccaacc cgcagcacat gagggagaaa
      421 tattccggga aaccaaaggc cagggcgatt ttcgcgagag ggggggccac cagttggaga
      481 gctacgaccg ccagggtccc gccgatgaaa gaccccaggg cgctgatccc cagagccacg
      541 ccggcccgtc cgcggcgggc catctggtag ccgtcgatgc aggtgacgac cgaggccgcc
      601 tccccgggga tgttgaccag gatggaagtg gtggaaccgc cgtacatggc tccgtaataa
      661 atcccggcca gcatgatgat ggcggacacg ggaggaatat ggagcgtcgc cgggaagagg
      721 agggacatgg cggccaccgg ccccagcccc ggcaaaaccc cgaccagggt cccgatgaat
      781 gccccggcaa agcagtagaa tagattcgtc caagtaagag taatggaaaa gccataaaga
      841 atgttcccaa gtatatccat gaactcacct tagaattcaa agaacccttt gggcagggga
      901 gatttcaaaa gaatttcaaa aagagtatag gaggcgaccg aagacaggat cgaacctaac
      961 accacgatgt accatttctg gggttcgacc actcccaaga ggaaggctaa gaaaaaggtt
     1021 gtgctcagga ggaatcccaa atattccatg cccacggcat aggccagaag gccgatcagg
     1081 gtcaaaactg ccttgagacc ttttgttttg tcggccaaga aaggtttcct ttccttattg
     1141 ccttgtccag tccggcgcga ttgcaagaaa acaaccagag cgagaccggt caagatggcc
     1201 ccggtgataa agggcaaaaa gcccggcccc gggtttctaa aatttcccag ggagagcttt
     1261 accgaaccaa gacagatcaa aacacccaaa aagacccacg ccacgctgcc ccagctttct
     1321 cgattcattt ttttctccaa ggcagaggat caggagctct gagcaacccg atttggaggc
     1381 cctccgaaga gctgccgccc gccccctcga tcactgcttg acaatcctgt aaatcccgga
     1441 aaacaattca tactgccggc ccaaatgggc agtgtattcc tccgggcctt caagcccgat
     1501 ctccgctccg atcttatcca gacgatcgga aatagaatcg ccgtatttat ccgcggcctt
     1561 tttggctgcc aagtagattg cctccaccac ttctttcgga gtgtctttcg gcgccaggat
     1621 cccgtaaggg ctcgcactgt ggatggagaa ccccaattct ctcagggtgg gggcgtcggg
     1681 aagggccttc atccttgtgg gtttaaacac ggccagggcg cggagcgctc cggcccggag
     1741 atggggaaga gccggagcga tggcggtgga ggcgacctgc acgtgccctc ccagcagggc
     1801 agtaatcgtc ggcccgctgc cctgggaagg gatgtaggtc catttgattc cggcctcctt
     1861 gcacatggct tcaggaatca tatggttgct tccgaaggtc ccggagctgg agtaggtgac
     1921 cttgcccgga gattgtttgg aatcttcgat gaggtccttc agggttttcc acggagagct
     1981 ggactggacc accagaaggg aactggcctc caccatggcg cacaccggag caaaggattt
     2041 ccaggtatag cccacatcct tgtgggtgag agggaccacc acgatggagg aatgggaaga
     2101 ccccaccagc aggtacccat ccggcttgct ggccgccacg aatcccgctc ccacggagcc
     2161 cgctgccccg ggcttataca ccatgttgac cggctggccc agaaattcgg gcatcttctc
     2221 cagaaaaggc ctgagcaggt tatccgtatc tccgggttgg aagggaatga taacctggat
     2281 aggcttcacc ggaaatttct tctccgccgc ccagggggtc cccgcagccg aaaaaatcaa
     2341 aaagaaaacc gctagaatga tcaaaaccgc ccgccttttg ttcatgttac atgcctcctt
     2401 tccagttggg ggccatgggg aaccgggcgg ggaattattt aattcgatcc ccagaggaac
     2461 cggaagttca ccttggcctc cgcggacggc ctaggttttt tccctcatct cagcatatcg
     2521 aaccaaggtt cgaagggcct ggacgctttt cttggtgttc atgaaaaggg ggatcccggc
     2581 cccgcgaatg atctggtacc cttcctcggc cagatttccg gcgatccagg agatgaccat
     2641 gggcttatcg gtttcctggt aaatctccac gatatcttgg gccattttct tggctctttc
     2701 gcctgtagac atggtgatca tcaggatgat gctgtggatc gacttctccc ggaccatggg
     2761 gcggaggagc tttttgaagt attcccggtc cttggccaga aattgggaga acatggccgt
     2821 ggtatccacg ggattcaggg cggagccaaa atagggtagc atccggacat attcctctcg
     2881 cgtcgctccc tgaagctcgg gaacttccaa ctcgcccatt tcgcactgat cggccattaa
     2941 acccccggct cccccggagg tagagagaat tcccacgctc cttcctttgg gaaggcgccc
     3001 ctggaccagg acatttccga tttccagcag ttcatcgccg tcctgcactc ttaaaatcct
     3061 caggcgctga aagagggcct ccacttccgg atcgggtcca catacggccc cggtatgggc
     3121 ttgcgccgct cttttcccca taggggtcct tcccactctc aaggcgacga tgggttttcc
     3181 cgcccggcgg gcagcctcgg cggcctggat aaattttgct tcgtcgcgat atccctcgct
     3241 gtagttgagg atgacgctgg tcttggggtc ttccacgaga tagcggatat aatccgccga
     3301 ttccagggcc gcctcgttcc cgctgctgat ccagtagctg aacccgatgc ccagctgcat
     3361 ggcgttgctg aagagtgaac ctcccatggc cccgctttgg gtcacaaagg caatcttccc
     3421 ggcgaggagc tgttttcgct ccagggcagg ggtaaagctc agggtggcgc cttcatggag
     3481 attgacaacc ccctggcaat tggggccgca gaggatgagg cccgaccgcc gggccaggtc
     3541 tcgcagctcc gtttccatct cttccccctt gcccccgatt tcggcaaacc cggaggcaaa
     3601 gacgatcacg gagtgcactt tccgctccgc gcactctttt actgcgtcca gaacgctttc
     3661 tgccggcaga ccgacgacca cagcgtcgat ttcacctgga acttgggcca gggaagaata
     3721 acaggttagg ccagcaatct ctttgtattt ggggttgacg ggatagattt tccccccgta
     3781 tttatgctgg agaagaaagg caatgggttt ccccacaagc tttgtcgtgt cttgggaagc
     3841 ccccaggacg gcgatacttt tgggatgtaa gagaggatcg agcttagagt tggaaaagtt
     3901 catcggtctt tcattaccct cctaatagat agatatcatt ggcccttcag gtcaatggag
     3961 cttgaaaaat cttcccctga ctcttccctt ctccctagga tttcggggga taggacggcg
     4021 tgagaaggca taaggggtgc attttttttg cagcgtttag gttaagcctt aaattcgaaa
     4081 tctgcaatct ccatcatctt cctttgtact ggggtttcct tttttcgcgg aaggccgcta
     4141 ccccctcctt atggtcttcg gaagtcagag tgatagtttc gcaggcggca gccactttca
     4201 tggcagtttc caggtccatc tccagcccct tataaatctg caatttggcc agccgaatgg
     4261 cgatgggggg cccgttggcg atacgccggg cgaggtccat cgtttccttc gccagttcgg
     4321 aggccgggac cagcttgttc agcaagccga gccgctcggc ctccttagct tccatgaaat
     4381 ctccggtaaa aagcatctcg gcggctttag ggataccgat gatccgggga tagagccaag
     4441 tccctcccca ccccggaaag aggccgattc gggtaaaggc caccatgaaa cgggcattct
     4501 cggaaccgac ccgcaggtcg caggcgcaag ccaggtcaaa gcctccgccc acggcagccc
     4561 cgttgaccat ggcgatggtg ggtttctcca ggcgctggag tcccaggatg accccctgga
     4621 tgtttcggaa gccgcgacgg aattcctctg cacccctggg cttcctttcc ctttcttccc
     4681 cggacatcct ccccacgtcc gcgccagcgc agaaggcctt tcccgcgcca gtgaccacca
     4741 ggacccggac ttcgtcatcc tggtccagtt cctggagcgc ctggacgagc tccgcctcca
     4801 tttgcacatt taaggcattc atcctgtccg gccggttcat ggtcaagagg ccgattcctt
     4861 cttttttctc gagcagtagg gtctcaaaag gcatattcct ctccttatca tctaaggcag
     4921 ttaagcgcaa ggggtcacga cttgcaactt aaggcttttt ttctgacttc tgtattctga
     4981 ttttcaatta ccgggtttcc ttccaaatgc ctttttcctt taaatatttc acggcaccag
     5041 gatggaaggg gatgtatttg gtggtgtatt cagccccccg atacatattc tcaaggctcc
     5101 attgcctagc ctggggatga atcgcgtcct tttccttcgg atgatcgtaa agggccttca
     5161 tcatctgata gaccaggtcc tcgctcattt cctggcggca gaaaatggcc gtgacaatgc
     5221 cccgggtaag ggtatccgta tcgatcccct gataggtccc cgccggaatc accaccttta
     5281 cgtagaacgg gtatgcgtcc atgatcgcct tcatttcctg ttcactgtag gggatcaagc
     5341 ggatcgggac cgtcctggcc aggtccagga gactggccac gggatagccc gcggagatca
     5401 ctcccacatc aacggttcca tccttgagcg cggtgatgga ctcggtgaag gaaaggtaag
     5461 cgggcttgaa atcctcaaga ccgagcttgc cggccgcccc aagctccatc ttggaggaaa
     5521 ccagggtgcc actgccgggg gacccaaggg ccactctttt gcccctgaag tcagagatgc
     5581 tcttgatggg ggaatccttg cggacaaacc agtgccggtc gctggtatgc cccaagctga
     5641 ccagtcgtac cccgcttagg tccattttct tttccagcgc cggggcgatg acgtggatcg
     5701 agatcagccc taggtccatc tttttccgta tcacgtaatg gaagttctct tcagaggcgg
     5761 cggtggattc ggcaatcacc cgcacgccgg gtacataccg gttgaagagg ttagcaaatc
     5821 cagccccgat gaaataaaaa gtaccccctg gattgccggt ccccagagaa aggtattttc
     5881 tctgggcggc tgcttctccg gcggcgaggc ccaaggaaac gctccccatg attgccaatc
     5941 cgacaatgac gcggatgaaa tatctgctca tgattgcctc ctttggtgga gatcagagtt
     6001 caaaaaagga tgttt
//
LOCUS       DASPRR010000301         5145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2164786, whole genome shotgun sequence.
ACCESSION   DASPRR010000301 DASPRR010000000
VERSION     DASPRR010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164786"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VEL68_11440"
     CDS             <1..331
                     /locus_tag="VEL68_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXZ36622.1"
                     /translation="VIARALRDAGMEVIYTGLRQTPEKIASAVVQEDVDALLMSILSG
                     AHDYLFPRVMQLLKEKGVKDILVLGGGVIPEEDIPALKAVGVKDVFGPGTSTENIVKY
                     VQENVKL"
     gene            444..1628
                     /locus_tag="VEL68_11445"
     CDS             444..1628
                     /locus_tag="VEL68_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXZ36623.1"
                     /translation="MRPLEGITILDLTRLLPGPYGTMLLGDLGAEVIKIEEPEQGDYA
                     RWYPPHINQVGSRHLLLNRNKKSITLNLKKPEGRSILLKMVEKGADVLIEQFRPGVMD
                     RLGVGYKDLEKVNSRLVYCALTGFGQDGPYRDLAGHDINYIGIGGILGITGPKGGPPA
                     LPGTQIADLVAGGLYAVIGILTALMGRLKTGRGQFVDVSMLDGVVSLIPDSAALFFAE
                     GESPRMGERRLTGGLPQYQTYRTKEGKYLAVGALEDKFWANLARALGKPDWAQRVPKE
                     KEPRTQEIKEEMIRIFLTKTRQEWLDILMKEDTCVTAVHSLEETFSDPHVLHRNMLVE
                     VPHPKAGRIRQIGVPIKFSETPGEVLTPAPEIGEHTEEILKGLGYNQQSIERLRKEGV
                     IR"
     gene            1734..1973
                     /locus_tag="VEL68_11450"
     CDS             1734..1973
                     /locus_tag="VEL68_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HXZ36624.1"
                     /translation="MAVSAYVLIRVEADKTKTAFEALTKLKEVKSAHTVTGPFDIILL
                     VEARNLHELGEVILTKIRGTDGVSRTMTCVTVEKG"
     gene            2191..2820
                     /locus_tag="VEL68_11455"
     CDS             2191..2820
                     /locus_tag="VEL68_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HXZ36625.1"
                     /translation="MKKELEFRFVANKVYTIPGRGTVVTGKVERGSVSVGDEIGFIGS
                     DGQLSRAVVSAIEVSRRLVEEAQAGQEASLLLEGSRKGQIVQGTVLMEVPEAAPVPVR
                     PAATETGPSEPVPPSYPSPVPRPIAPSSGMGRFIIYILVGLLILFAFLFIQGKLDPKN
                     WDPRKWDPKKKRASIQRSVVSDHLSYFIPLSVSGGEGFIVSSLRSERLD"
     gene            2943..3956
                     /locus_tag="VEL68_11460"
     CDS             2943..3956
                     /locus_tag="VEL68_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXZ36626.1"
                     /translation="MQKVRLGRTNISVKKLGWGGIPIQRVSDDQAVSVVQAAMELGVE
                     IFDTARGYTNSERRIGLALQKTSRPVILSTKSANRTEKIYDDVQESLRQLQVKKLHIY
                     HLHNVSKTEDYEKVMAAGGAYQGLQKARAEGLIDHIGLTSHSLDILERVLKEDRFEVV
                     MACYSFLEPDAARRILPLARAKDVGVLTMKPLSGGVIEDAGLALRYVLSTPDIVPIPG
                     SETMERVRENWKVYAEGRPLSEADQDRIREVRQQFDKQFCRRCDYCQPCSQGINIQLM
                     MGIKSVIRRFGNPDEIAWVKGLAEKARDCTECGECLPRCPYQLPIPELIKKNLEWYDS
                     LKK"
     gene            4207..4512
                     /locus_tag="VEL68_11465"
     CDS             4207..4512
                     /locus_tag="VEL68_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36627.1"
                     /translation="MGFFFILFYVVPLFVALWVFFDSQKHGYTFWQGLLWAIGVFFLL
                     IVFLPLYFFYRNKRRKPALAPGRAPTPTYATRCFYCGQPYSGDPKTCPHCGQNLKIQ"
     gene            complement(4566..>5145)
                     /locus_tag="VEL68_11470"
     CDS             complement(4566..>5145)
                     /locus_tag="VEL68_11470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ36628.1"
                     /translation="GEPLLLKRDEHPRADTSLEALGKLRPAFRAGGTVTAGNSSGIND
                     GAAAVLLMSDRKARELGLKPWARVLTSAAAGVEPRVMGLGPISATRKALARAGLTLDQ
                     IGLIELNEAFAVQALAVMKELGFRHEITNVNGGAVALGHPLGCSGARLMTTLVHEMRR
                     RASKEKRPYYGLATLCVGVGQGEATIVEWIGY"
BASE COUNT         1159 a         1379 c         1505 g         1102 t
ORIGIN      
        1 ggtaatcgcc cgggcgttgc gcgacgccgg gatggaggtg atttacacgg gactgcgcca
       61 gacgccggag aagatcgcct cggccgtcgt ccaagaagac gtggacgctc tcctcatgag
      121 catcctgtct ggcgcccatg attatttatt tccccgggtg atgcaactcc tcaaggaaaa
      181 aggggttaag gatatcctgg tcctgggggg aggggtgatc cccgaagagg atatcccggc
      241 gctgaaggcg gtgggcgtca aggatgtctt cggaccggga acttctacgg agaacatcgt
      301 caagtacgtg caggaaaacg tgaagcttta aacacagtgt cagtgtgagt gtcagtgaaa
      361 aacaaggata caggttcagt ctttgcggag aggacctttc tttttctgac actgacactc
      421 acactaatca ctaggtatta tttatgagac ctttagaggg aattaccatc ctggatttaa
      481 cccggctctt gccggggccc tacggaacca tgctcctggg agaccttgga gccgaggtca
      541 ttaaaatcga agagcccgaa cagggggact acgctcgctg gtatcctccg catatcaatc
      601 aagtgggctc gcggcatctt ttgctgaacc gcaataagaa aagcatcact ctgaacctga
      661 aaaagcctga ggggcgatcc attcttttga aaatggttga aaagggggcc gacgtcttga
      721 tcgaacaatt tcgccccgga gtgatggacc gcctgggggt cgggtataaa gacctggaga
      781 aggtcaactc ccggctggtc tactgtgcac tcaccgggtt cggccaggac ggaccgtatc
      841 gggaccttgc cgggcacgat atcaactata tcggaatcgg cggaatttta ggcatcaccg
      901 ggccgaaagg cggcccaccc gccctccccg gaacccagat agccgacctg gtggctgggg
      961 gactttacgc ggtgatcggg attctgactg ctctcatggg ccgcctgaaa accggccggg
     1021 gtcaattcgt ggatgtatct atgctggacg gggtggtctc cttgattccg gattccgccg
     1081 ctctcttttt tgccgaaggg gaatctcctc gaatggggga acggaggcta accggcggtc
     1141 tgccgcagta ccagacctac cggaccaaag aagggaagta tttggccgtg ggggccctgg
     1201 aagataagtt ctgggccaac ctggcccggg ccctgggtaa gccggactgg gctcaacggg
     1261 ttcccaagga aaaggagccc cggacgcagg agatcaaaga ggaaatgatc cgcattttcc
     1321 ttaccaagac ccgacaggaa tggctggaca tcctgatgaa agaagatacc tgcgtaaccg
     1381 ccgtccattc cctcgaagag accttctccg atccccatgt ccttcaccgc aatatgctgg
     1441 tggaggtccc acaccccaaa gccgggcgga tcagacagat tggggtcccc atcaaatttt
     1501 ccgagacccc cggtgaagtt ctgacgcctg caccggaaat aggagaacac accgaagaaa
     1561 tcctgaaggg attgggatat aatcaacaga gtatcgaacg gctgcgcaaa gaaggagtca
     1621 taagatagcg atcagctttc agccatcagg tgtccgcccc atgaaagaga tgtttttttt
     1681 ggctgatcgc tgaaagcgga tggctgaggg ctgtcgttca aaaggaggtt tctatggctg
     1741 tttcagcgta tgtactaatc cgggtagagg cggataaaac caagacggcc tttgaggcgc
     1801 tgaccaaact caaggaagta aagtcagctc acacggtgac gggccctttc gacatcattc
     1861 tgttggtgga ggccaggaac ctccatgaac tgggagaggt catcctgact aaaatccggg
     1921 gaacggacgg ggtttcccgg acaatgacct gtgtaacggt agagaaaggg tagggccgaa
     1981 aatcgggagc gggggctctg agaaacaatg gcttttctct cgcccgctcc gctggcccgc
     2041 ggagtacaca gagaaagtca aaagtgatca aaatgcaatt gcagaaagta ttcggtcaat
     2101 catttgtttt tctttcttct ctgcgaactc tgcggcctct gcgagagaca tttttttgaa
     2161 ctagatggaa gagccggcgg aggaaggaac atgaaaaagg aacttgaatt ccggtttgtg
     2221 gccaacaagg tttacactat ccccggaagg gggacggtgg tgaccgggaa agttgagagg
     2281 gggtctgtgt cggttggcga tgaaatcggg ttcatcggct cggacgggca gctttccagg
     2341 gccgtcgtat cggccatcga ggtctctcgc cgcctcgtgg aagaggccca ggcggggcag
     2401 gaagccagcc ttctcttgga gggttcgcga aagggccaga ttgtgcaagg gaccgtattg
     2461 atggaggtac cggaggcagc cccggttccg gttcgcccgg ctgccacaga aacgggtccc
     2521 tccgaaccag ttcccccttc ttatccttcg cctgtaccca gacccatcgc cccttcctcc
     2581 ggaatggggc gtttcatcat ttatatcctg gtcggtctct tgattctttt tgcttttctt
     2641 ttcatccagg ggaaacttga ccctaaaaat tgggacccga ggaaatggga cccgaaaaag
     2701 aagcgagcca gcattcagcg gtcagtggtc agcgatcacc tctcttattt cattcctttg
     2761 tccgtctcgg gaggagaagg cttcatcgtg agctcccttc ggtctgagcg gctcgactga
     2821 gcccgtcgag agcctctggg tcgaacgact cgccgaagtc tcagggtcga agacagccga
     2881 acgaataggt tgggggggat tctaatcccc tacggccggg cgcggttcgc taaaggaatt
     2941 ttatgcagaa agtcagactg ggtcggacga acatttcggt taagaaatta ggatgggggg
     3001 gaatccccat tcagcgagta agcgacgacc aggcggtatc agtggttcag gctgcgatgg
     3061 agctgggggt ggaaatcttt gacacagccc ggggatatac caacagcgag cggcgcatcg
     3121 ggctggcgct ccaaaagact tcccggcccg tcatcctttc caccaagtcc gccaaccgga
     3181 cggaaaagat ctatgacgac gtgcaggaga gtctccgcca gctccaggtg aaaaaactac
     3241 atatttatca cctccacaat gtatccaaaa cggaggatta cgagaaagtc atggccgccg
     3301 gcggggccta ccaaggcctt cagaaagccc gggctgaagg gttgatcgat catatcgggc
     3361 tgaccagcca tagcctggat attctggaga gagtgctcaa ggaggaccgg tttgaggtgg
     3421 tcatggcctg ttacagcttc ctcgagccgg acgccgcccg caggatccta cccctggcgc
     3481 gggccaaaga cgtgggagtt ctgaccatga agcccctttc gggtggagtc atcgaagatg
     3541 ccgggctggc ccttcggtat gtcctgtcga cgccggacat cgtccccatc ccgggatcgg
     3601 agacgatgga gagggtacgg gagaactgga aggtctatgc cgagggccga ccccttagtg
     3661 aggccgatca ggaccggatc cgagaggtcc ggcagcagtt tgacaagcaa ttctgccgcc
     3721 gctgcgatta ctgtcagccc tgctcgcagg ggatcaacat tcagctcatg atgggaatca
     3781 aatcggtcat ccggcgattc gggaaccccg atgagatcgc ctgggtaaag ggattggcgg
     3841 agaaggcccg ggactgcacg gagtgcgggg aatgcctgcc ccgctgtcct taccaactgc
     3901 ccattccgga actgatcaag aaaaatctgg agtggtatga tagtctaaaa aaatagcgat
     3961 cagctctcag cattcagctt atttttttta ttaaagattg ctgatctcgc aaaaaatgga
     4021 atttccatag aattcgtcat tccggcggag gctcgtagag tcgagtctcc gaccggaatc
     4081 cagttattta aaaaatttct ggaccccggc tttcgccagg gtgaccctcc aaaagactct
     4141 ttccgagatc atcaggattg tttgctgacg gctgaaagct gatagctgtt tttgggagaa
     4201 tgcagggtgg gtttcttttt catcctcttt tatgtggttc ctctgttcgt ggccctctgg
     4261 gttttttttg actcccagaa gcacgggtat accttctggc aaggccttct ctgggccatt
     4321 ggcgtcttct tcctcctgat cgtcttcctc cccctctact ttttctaccg gaataaaaga
     4381 aggaaacccg cccttgcccc aggacgggcg ccgactccga cctatgcgac ccgctgtttc
     4441 tattgcggcc aaccctattc gggtgacccg aagacctgcc cccattgcgg ccagaacctg
     4501 aaaatccagt aatagaatat aggggaatca ccccaagcgt gtgatctccc cgcaggaatc
     4561 ggttttcaat acccgatcca ctccacgatc gtcgcttcgc cctggccgac ccccacgcac
     4621 agggtcgcca gaccataata aggccttttt tccttggacg cgcggcgccg catttcatga
     4681 accagagtag tcatcagtcg ggctccgctg catcccagag ggtggcccag ggccacagcg
     4741 ccgccattga cattagtgat ttcatggcgg aaccccagtt ctttcatcac cgcgagggct
     4801 tggacggcaa aggcttcatt cagttcgatc aggccgatct ggtcaagggt cagcccggct
     4861 cgggccaggg ccttgcgggt ggcggagatg gggccgaggc ccatcacccg gggttccact
     4921 ccggcagcag ccgaggtcag gacccgggcc cagggcttca acccaagttc acgggctttg
     4981 cgatcgctca tcagcagaac ggcggctgcc ccgtcgttga tccctgagga atttcccgcg
     5041 gtcaccgttc cgccggcgcg aaaggcgggc cgcagtttgc ccaatgcctc cagggatgta
     5101 tcggcccggg gatgctcgtc ccgtttgagc aataggggtt ctccc
//
LOCUS       DASPRR010000302         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_222334, whole genome shotgun sequence.
ACCESSION   DASPRR010000302 DASPRR010000000
VERSION     DASPRR010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222334"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VEL68_11475"
     CDS             complement(<1..1019)
                     /locus_tag="VEL68_11475"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HXZ36629.1"
                     /translation="MAQSGYGKRPQKGRIKDKTVLLIGTMDTKEEELLFCRELIRRTG
                     FKVLLLDAGILKDPRVAPDINRREVARAAGVSDLESFLALGDKGKCIETMIRGAAAKT
                     QELLGQKAFHGILGMGGGQGTDICTSAMRSAPRGMPKLMVSTVASGKATFGPYVGTKD
                     IAMMHSVVDLQGLNFVTRRILSNAVGAICGMVGALEEADTKPRGVPVALSMLGTTTPG
                     ALRVKASLERRGYEVVTFHQNGTGGIAMEEMIREGAFKGVLDLNLHEIGDRFVGGLHS
                     DPLHERLEAAGEVGIPQVIAPGSINYAVWGPFPSLSGELKSRKYIIHNPNMTLVRLSP
                     EELRSV"
     gene            complement(1022..1387)
                     /locus_tag="VEL68_11480"
     CDS             complement(1022..1387)
                     /locus_tag="VEL68_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ36630.1"
                     /translation="MDGGFHWSEIAWERVNDSIDRKVVMGERLMMVMYRFSPNQSWPE
                     EVHEAEQGGYVLRGKIELTLPAQGEKHLLGAGDGYLIHSRIPHSWRVLEETVLIDFFS
                     PPRRELYQKKFAPNAKEGQ"
     gene            complement(1656..>2492)
                     /gene="mvk"
                     /locus_tag="VEL68_11485"
     CDS             complement(1656..>2492)
                     /gene="mvk"
                     /locus_tag="VEL68_11485"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HXZ36631.1"
                     /translation="TADAEVAQTASGGWEIRDQRREAKGYKEEKKGMQIESLERVFQY
                     LRFRPEHLRIRLSGDLPAMSGIGATGASSVAIVRALAEEFKLRLSDDEVNAAAYQAEI
                     AYHGPTTSGIDNMISTYGAIIALTRGEPNVVRKMKLKEPVEVVIGDTGIIANTKTLLA
                     ALGERKRNSPERYGRILQEARQIAAEATVNIERFDLKGIARLMDRNQSLLKEADISCP
                     ELDLLVDLSRNAGALGAKLTGSGGGGCMLALTPGKDLQEKVASAIESRGFQALRSRLG
                     VN"
BASE COUNT          461 a          798 c          666 g          567 t
ORIGIN      
        1 acgctgcgca gttcctccgg gctcagccgc accagggtca tgttgggatt atgaataata
       61 tattttcggg acttcagttc cccggacagg ctaggaaaag ggccccacac cgcataattg
      121 atggaccccg gagcgatcac ctggggaatt cctacctccc ccgccgcttc cagccgctca
      181 tggagaggat cgctgtgcag ccccccgaca aagcggtccc cgatttcatg aaggttcagg
      241 tccagaactc ccttgaaggc tccttcgcgg atcatctctt ccatggcgat ccccccggtg
      301 ccgttctgat gaaacgtgac cacctcgtat cctcgccgct ccaagctcgc cttcaccctt
      361 agggctccgg gggttgtcgt ccccagcatg gataaggcaa ccgggactcc ccggggtttc
      421 gtatctgcct cttccagggc cccaaccatt ccgcagatgg cccccactgc attggacaga
      481 atgcgccggg tcacaaaatt cagcccctgg agatcgacga cggaatgcat catagcaata
      541 tctttggtgc ccacgtaggg gccgaaagta gccttgcccg aagcaacggt ggagaccatg
      601 agtttgggca ttccccgcgg ggcgctccgc atggccgacg tgcagatatc ggtcccctgc
      661 ccccctccca tccccaggat cccgtgaaac gccttctggc ccaggagttc ctgagtcttg
      721 gcggcagctc cccggatcat ggtctcgatg cactttccct tgtccccaag ggccaagaag
      781 ctctccaaat cggagactcc ggccgctcga gccacttccc ggcggttgat gtctggggca
      841 acccgtgggt ctttaagaat tcccgcgtcg aggagaagga ctttgaaccc cgtgcggcgg
      901 atgagctccc ggcagaagag gagctcctcc tctttggtgt ccatggtccc tatgagaaga
      961 accgttttat ccttgattct ccctttttgg ggcctttttc cataacccga ctgcgccatt
     1021 ttcattggcc ttctttggca ttgggggcaa acttcttctg atacagttcc cgccgcgggg
     1081 ggctgaagaa atcgatcaga acggtttctt ccagtactct ccaggaatga gggatacgcg
     1141 aatggatcag gtagccgtct cccgcgccaa gaaggtgctt ctcgccctgg gcgggcaagg
     1201 tcagctcgat ttttccgcga aggacataac cgccttgttc ggcttcgtga acctcctctg
     1261 gccaagactg gttcggggaa aagcggtaca tgaccatcat cagccgttct cccataacca
     1321 ctttccggtc gatagaatcg ttcacccttt cccaggcgat ctccgaccag tgaaatccgc
     1381 cgtccactcc ctcttcctcc ttggttgcaa aaaattatcc ctatattcgc ctgcggctgc
     1441 ccccccaccc caaccctccc ccttaggaga ctgtgtcgta attcgtcatg ccggcagaag
     1501 ccggcatcca ggattcaaat aaattgaata aaaccgaaca ctggattccg gctttcgccg
     1561 gaatgacacc cttcccctta gttgcgacac agcctctaga ggggggaggg aagggtgggg
     1621 gtgatcttca gaaatatttt tttgcagcgt ttaggttaat tcacccccag cctcgaccgg
     1681 agcgcttgaa acccccggga ttcgattgcc gatgccacct tctcctgcag gtcttttccc
     1741 ggggtcaagg ccagcatgca accgccgccc cccgacccgg tgagcttggc tcccagcgcc
     1801 cccgcattgc gggataggtc caccaggagg tccaactccg ggcacgagat gtctgcctcc
     1861 ttcagcaggg actgatttcg gtccatgaga cgggcgatcc ccttcaagtc gaacctttcg
     1921 atgttgaccg tggcctctgc ggcgatctgc cgggcttcct gcaagatgcg tccgtaccgt
     1981 tcgggagaat tcctttttct ctccccgagg gcggcaagga gagtcttggt attggcgata
     2041 attcctgtgt ctccgatgac cacctcgacc ggttccttta atttcatctt ccgcacaacg
     2101 ttgggctccc cgcgggtgag ggcgatgatc gcgccgtagg tggagatcat attgtcgatc
     2161 ccgctcgtgg tcgggccgtg gtaggcaatt tcagcctggt acgcggcggc gttgacctcg
     2221 tcgtcggaca gccggagctt gaattcttcg gcgagggccc gcacaatggc caccgaactc
     2281 gctcccgtcg cgccgattcc gctcatggct ggcagatcgc cgctgagccg aatccgcaga
     2341 tgttccgggc ggaaccggag atattggaag accctttcca gggactcgat ctgcatcccc
     2401 tttttttctt ccttatagcc tttggcctcc cgacgttgat ccctgatctc ccagcctccg
     2461 gatgcggtct gagccacctc cgcgtcggcg gt
//
LOCUS       DASPRR010000303         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1517533, whole genome shotgun sequence.
ACCESSION   DASPRR010000303 DASPRR010000000
VERSION     DASPRR010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517533"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..>2906)
                     /locus_tag="VEL68_11490"
     CDS             complement(245..>2906)
                     /locus_tag="VEL68_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXZ36632.1"
                     /translation="DFIREIFRFWRLKDKSGLRRFLPAEVHHPLKTEGPFIDEVCGIL
                     RELFLQKGFQKEEDLLRLSESEVESILAQLPGFSDPEKKRVDLLIRIYRLINQKYHLG
                     FQEIRYHLQQASRWGFEGLDELQKVLDRNDTLECLEAILTYLEGLKEIIISAERFEAR
                     EDIYRKRHIAVDIPSMYGRYREKKFDALGLTFRLENLANIYFERLIDSMDLSFITRAT
                     FFQIIRVIHYFFRAMQLDGIFSQRLDTQLKLLGKSLEIKRFTFTQYLDIFRGFSEAVK
                     DILSVYYINAHQNNLSNLIRRMGRENIQAKYLPPPGEELSPNEFTHQISERFLRDLVS
                     STFGLQYLDNFLARIQQILSEQKETLSEADLDLLMTYDAKKVTCFIHNPNPLTNDLIH
                     LGSKGYNLLVLASEGIHIPPGFIVTTEVFRCLRIIEQYKHARDHFEREIRSGIQQIEK
                     MTGNEFGSTRRPQLLAVRSGATISMPGMMATLLNVGINEEIVESMARSTGEAWFAWDN
                     YRRFIQSWGMSFGMQREVFSEIMRKYKAKYHAEKKRQFTGEEMKQLALAYRSAVEERH
                     ITLYEDPWAQLHVAIQQVLNSWESKNAREYRQIRGISDYWGTAVIVQSMVFGNLSLYS
                     GSGVFFTAHPYRQVRRVGLWGDFTPGNQGEDIVGGLVSTYPISKEQREAAGEEGDLSL
                     EEDFPEIYRQLFSISKTLVYDRRWNPQEIEFTYERPDASGLFILQTRDMVSAKRERFE
                     VFSPSPELEGSFIGKGIGVSGGALSGRVVFTLEDIQRFRKEDPAIPLILIRSDTVPED
                     IRELSLTEGLLTAKGGQTSHAAIVAFELDKTAVVGCHHMVVMENEGRSHINRTLLQRG
                     DWISLDGRRGFVYLGKHETKDEGEPYHSHI"
BASE COUNT          570 a          831 c          854 g          651 t
ORIGIN      
        1 cggagaaagc ggctctggct gcgtggtgcc acagggtgag cttgccaatt cgtcccaatc
       61 cattgattcc caattttagc ggaagttttt ctccgccctg cgccgggtcc tttccgtgcc
      121 gcggtccata gctcatacat gcacccccaa gattatgttg attgaggcga tagcggggcc
      181 ttttccataa atcctgtctt taaagccgat gggttcagtc ccccagctga ccaagaaagg
      241 aattttaaat gtggctatgg tacggctctc cctcgtcctt ggtctcgtgt ttgccgaggt
      301 agacaaaacc ccttctgccg tccaggctga tccaatctcc ccgttggaga agggtccggt
      361 tgatgtggct gcgaccctcg ttttccatga cgaccatatg atggcacccc accaccgccg
      421 ttttgtcgag ctcgaaggcc acaatagcgg catgggatgt ttgtcccccc ttggcggtca
      481 gcagcccttc ggtaagggat agttcgcgga tgtcttccgg gacggtatcg gatcggatga
      541 ggataagggg aatggccggg tcctccttcc ggaaccgctg gatgtcttcc agagtgaaga
      601 ccacccttcc tgagagggcc cccccgctga ccccgatccc cttgccgatg aagctcccct
      661 ccaattccgg ggaaggggag aagacctcga acctttccct cttggcggag accatatccc
      721 gggtctggag gatgaaaagg ccggaagcat ccgggcgctc ataggtgaat tcaatctcct
      781 gggggttcca cctccggtcg taaaccaggg tcttggagat ggagaagagc tgcctgtaaa
      841 tctccgggaa atcctcctcc agggataggt ctccctcctc cccggccgcc tcgcgctgtt
      901 ccttggatat agggtaggta ctgaccaggc cgccgacgat atcctctccc tggtttccgg
      961 gggtgaaatc tccccacagg cctacccgcc ggacctgccg ataaggatga gcggtaaaaa
     1021 aaacaccgct gcccgagtac aggctgaggt ttccgaagac catggactga acgatcactg
     1081 ccgtgcccca gtagtccgag attccccgga tctggcggta ctcccgggcg ttcttggact
     1141 cccaggaatt cagaacctgc tggatggcca cgtggagctg ggcccagggg tcctcataga
     1201 gggtgatatg cctttcctcg acggcggaac gataggcgag ggccagctgc ttcatctcct
     1261 ccccggtgaa ctgccttttc ttctccgcgt ggtacttggc cttgtacttg cgcatgatct
     1321 cgctgaagac ttcccgttgc atgccgaaag acatccccca ggactggatg aaccgccggt
     1381 agttgtccca ggcgaaccag gcctccccgg tggaacgggc catgctctcc acgatctcct
     1441 cgttgattcc cacgttcagc agggtggcca tcatccccgg catggagatg gtcgccccgc
     1501 tgcgcaccgc cagaagctgg gggcgccggg tgctgccgaa ctcgtttccg gtcatcttct
     1561 cgatctgctg gattccggac cgaatctccc gttcgaagtg gtcccgggcg tgcttgtact
     1621 gctcgatgat ccgcaggcag cggaagactt cggtcgtgac gatgaagccc gggggaatat
     1681 ggatgccctc ggaagccagg accagcaggt tatacccctt gcttcccagg tgaatcaggt
     1741 cgttggtcag gggattggga ttatggatga aacaggtgac cttcttggca tcgtaggtca
     1801 tcagcaggtc caggtcggcc tcactcaggg tctctttctg ctcggagagg atctgctgga
     1861 ttcgggccag gaagttgtcc aggtactgga gcccgaaggt gctggagacc aggtcccgca
     1921 ggaagcgctc gctgatctgg tgggtaaact cgttggggct taattcctcg cccggcgggg
     1981 ggaggtattt agcctggatg ttctcccgac ccatccggcg gatcaggttg gaaaggttgt
     2041 tctggtgggc gttgatgtag tagacggaaa gaatgtcttt aaccgcttcc gaaaaccccc
     2101 ggaagatgtc caaatactgg gtgaaggtga accgtttgat ctccagggac tttcccagga
     2161 gcttgagctg ggtgtccagc cgttgggaga agatcccgtc caactgcatg gcccggaaga
     2221 agtagtggat gacccggatg atctggaaga aggtggcccg ggtgatgaag gaaaggtcca
     2281 tcgaatcgat gagccgttcg aagtaaatat tggcaaggtt ttccagacgg aaggtgagcc
     2341 ccagagcgtc gaacttcttt tcccggtagc ggccgtacat ggaggggatg tccacggcga
     2401 tgtgccgctt gcgatagatg tcttcccggg cttcgaaccg ctccgcggag atgatgatct
     2461 ccttcaaccc ttccaggtag gtcaggatcg cttccagaca ttccaaggtg tcgttgcggt
     2521 ccaggacctt ctgcagttcg tccagcccct cgaagcccca ccgcgaggcc tgctgcaggt
     2581 ggtagcggat ctcctgaaac cccagatgat atttttggtt gatcaggcga tagattctga
     2641 tcaaaaggtc cacccgtttc ttttccgggt ctgagaatcc aggcaactgg gccaggatcg
     2701 actccacctc cgattcgctc aggcgcagga ggtcttcctc cttctggaag cctttctgca
     2761 gaaaaagttc ccgcaggatt ccgcacacct cgtcgatgaa agggccctcg gtcttcaggg
     2821 gatgatggac ttccgcgggc aggaaccgac ggagcccact tttgtccttc aaccgccaga
     2881 aacggaaaat ttcccggatg aaatct
//
LOCUS       DASPRR010000304         8067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2165267, whole genome shotgun sequence.
ACCESSION   DASPRR010000304 DASPRR010000000
VERSION     DASPRR010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165267"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VEL68_11495"
     CDS             <1..190
                     /locus_tag="VEL68_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36633.1"
                     /translation="VIEKGKLVYDDSVVLEEDWEELEKINWNTGKLYSLDSIRKDRQA
                     AKERFAECQDSRKNGKGG"
     gene            174..911
                     /locus_tag="VEL68_11500"
     CDS             174..911
                     /locus_tag="VEL68_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ36634.1"
                     /translation="MGKEDEDMTLQGKKVVIIGGSSGIGLATAKAAAAEGAQVIIASR
                     SEEKLRKAAAEIKGKVESITVNTLEESLVKALFVRVGEFDHLATPGSEAAWGPFLEMD
                     LHPAKSGFDSKFWGQYLAAKYAAPKIRQGGSITLFSGAYSQRPGRGASLLSAINSAVE
                     GLGRALALELSPIRVNVISPGLVDTPIYSGMPEDKRKAMFNAVAGANLVKRIGRPEDI
                     AETALYLMNNGYTTGSTLYVEGGIMLR"
     gene            complement(1041..2804)
                     /locus_tag="VEL68_11505"
     CDS             complement(1041..2804)
                     /locus_tag="VEL68_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXZ36635.1"
                     /translation="MAKMKGGQIVAECFEKLGVEYYFGYNGHGIWNILDALIDKPHLK
                     GIQPKHEITAVHMADGYFRARHKVAPVMASVGPGVLHLLPALGNAMLDSSGVLIIPTG
                     PPTHYLDKGSLEELSVHGGDDTPNMFRPITKRIWYAMRPDILAYQIMQAYKLAGTGRP
                     GPTAVYIPFDVSSHEVEIDSTSTWEKDHLTTRAGGNPEAIQEAARLFAQAQKPLLLVG
                     GGAHIAEAWIEVKEFVDTLQVPVVTTMPGKGALTEDHPLCLGPAGRSGWGSSVKATQA
                     ADVVLSIGCRFCDAHTSGWRKGVVYNFPETKLIQVNIDQLDIGRSCLADVGILADGKI
                     ALQQLLEAVKPLWKKQEKARQPWVAQTKKWREEWLAKIESDYQGTEDPVSTNRLAVEV
                     NRALPKDANIVTDTGDIQQAYEGLAVVHTPNTFFNNSGMAQMGWAAGAVMGVKLARPN
                     KVALSIVGDGSFIMSSYVLATAVEYKIPVIWVVINNFGQSIERHGQLINYGREVWSSF
                     QKDGKPYNPDFVKIAEAYGVKGKRIHASGEIKPAILEAIRLNEPYLIEVVTDRNSPTY
                     FCPGVTRGYPIRWDKLAYLKQ"
     gene            2997..4130
                     /locus_tag="VEL68_11510"
     CDS             2997..4130
                     /locus_tag="VEL68_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36636.1"
                     /translation="MKKLIWALAVGSFFFGAFLPAGWTAEPVKIGVGLPLTGPLAFLG
                     TEFLKGAQLAAEEINKSGGILGGRKIELVVRDHKGIPAEAVTLAKRLIAEDKVSVIDI
                     DLPSSANIAVHVVSKEMKVVQLTGFGFAPDVTDKGHPYHFRVCTRAESLTRVLGEYLA
                     ALPNNKKIAMVAPNDDYGRGDLASMIAVFKKIGNPEVVYEAYYERNQTDFNTILLKMK
                     SLNPDCYYINVRWPASAIVLQQMEDLAMLKGKYLSSSVNFFNKDLAKRVGPLMEGAVM
                     TVSWAPVFKDEESQKLVKAYRAKYGDDPNDSVSLGWSPIMVAAMGINKAGTDKDQEKI
                     RLAIKGLKYNSPQGPINGFDDKGDSGVPAYVLQFKNGEYHLIK"
     gene            4383..5261
                     /locus_tag="VEL68_11515"
     CDS             4383..5261
                     /locus_tag="VEL68_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36637.1"
                     /translation="MWEATFILAIQQIASGLVMGSMYALMSIGMMLILGIMTVINMAH
                     GEFYMLGGYVSFFLMTQMGLPYLVSLPVSMVVVGAIGIASEKMTIRPLVGRPWFSTFL
                     TTFGLSMILRDGAQIIWGVDPREIVSPFSHKRVVLGPIFLTEHRIFVFVVGVVVIGLL
                     YLFIKKHKVGMAMRAIVRDREASALMGINLNAMNSFTFALGAGTAALAGALLGAIFNV
                     LPSMGELPLVKGFAVVIMGGMGNVQGILFSGLILGVAESLSASFISLGLSDAIAFAIL
                     IIILLFKPAGLFGKRV"
     gene            5264..6241
                     /locus_tag="VEL68_11520"
     CDS             5264..6241
                     /locus_tag="VEL68_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36638.1"
                     /translation="MNRRKLLCLSGLVLILLLLPWLLQAAYTRHILVMALIFTFSAMG
                     LNVIFGFAGQTAFGQPAFFALGAYISSLLALKLGFPFWLCLLGTMVLSAGFGFMIGYP
                     CLQLRGIYFGITTMAFAQILYYIVSNWVELTRGPMGLVDIPPASFGFGDHFQFVFIGE
                     LRYYYLVLAASLVTLFFLSRLMETRLGRGFVAIRENQDLSASIGIHPFWVKMIAFMLA
                     CVITGMGGSFYAHYVKFVSPIEFQWYFIGLTFIMVITGGIGTIWGPFIGGAIFTVLPE
                     VFRAVETYRNILVGAVLILIVIFFPEGIMGKVAQWRGKDAQPALERKNA"
     gene            6234..6998
                     /locus_tag="VEL68_11525"
     CDS             6234..6998
                     /locus_tag="VEL68_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36639.1"
                     /translation="MLETQGLTVAFGGLVAVNQIDLSVSPGEILGMIGPNGAGKTTFF
                     NLLTGFLTPTSGRILFKGKDIAGLPPSRIASLGICRTFQITSVFPGLTVRENLLTGTY
                     CWTRTLFFDTLFHSQGYSRQGQEMEKHAGEILEFLHMEDRKDTVARNLSYGDQRRLEI
                     GIALASRREFLLLDEPAAGMNPEETQTLSQLIRQIRDKGITIFLIEHHMQLVMGICDR
                     IAVLNYGRKIAEGNPLEISRNPQVIEAYLGKEEEFA"
     gene            6991..7695
                     /locus_tag="VEL68_11530"
     CDS             6991..7695
                     /locus_tag="VEL68_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36640.1"
                     /translation="MLELKSIVAQYGHVRALKGISLTIHQGENVTLLGANGAGKSTTL
                     KCISGLLRISSGSILFQGERIDHLPPHKIIREGITQVMEGRRLFPDLDVLENLRLGAY
                     LSDDRQAIQQRMDRVFGYFPILKERQKQLASTFSGGEQQMLAMGRGLMASPNLLMLDE
                     PSLGLAPILVQEVGKIIRTINRDGVTLLLIEQNAQMALRLANRGYVMETGSIALEGTS
                     QELISNDKVRKAYMGL"
BASE COUNT         1620 a         2313 c         2354 g         1780 t
ORIGIN      
        1 cgtaatcgaa aagggaaagt tggtttacga cgactcggta gtcctggagg aagattggga
       61 ggagctggaa aaaataaatt ggaatacggg gaaactctat tctttggatt cgatccgaaa
      121 agatcgccag gcggcgaagg aacgttttgc cgaatgtcaa gatagcagaa aaaatgggaa
      181 aggaggatga agacatgacg cttcagggga aaaaggtggt catcatcggc ggcagctcgg
      241 ggatcggcct ggcaacggcc aaggctgcag cggcggaagg ggcccaagtg atcatcgcca
      301 gtcgttcgga ggagaagctg cgaaaagcgg ctgcagaaat caaagggaag gtggaatcca
      361 ttacggtgaa caccctggag gaaagtttgg tgaaagcgct tttcgtccgg gtgggagaat
      421 ttgatcatct ggctactccc gggagcgaag ctgcctgggg tcccttcctg gagatggacc
      481 ttcatccggc aaagtccggg ttcgacagta aattctgggg ccagtatctg gcggctaaat
      541 atgcagcccc caagattcga caaggggggt ccatcaccct cttttccggc gcctacagcc
      601 agaggccggg ccggggcgcc tctcttcttt ccgccatcaa cagtgccgtt gaaggcctgg
      661 gccgggccct ggccttggag ctttctccaa tccgggtcaa cgtaatttcc cccggcctgg
      721 tggatacacc gatttactct ggaatgcctg aggataaacg caaggctatg tttaacgccg
      781 tggccggagc gaacctggtc aagcggatcg gcagacccga ggatatcgct gagacggcgc
      841 tttatttgat gaacaatgga tacacgaccg gctccaccct ctatgtggag ggcgggatca
      901 tgctgcggta gtccctggta gggcgcgttc gcggaacgcg ctgctgcgac ctggggggtg
      961 gcgaaccgtt cgtaggggcg gcccgtcgtg tcgcccctac agagatgata aaaaagtccg
     1021 acctgatcgc ggatcagacc ctactgtttg aggtaagcca gcttgtccca gcggatcggg
     1081 tagccgcggg taacgccggg gcagaagtag gtcgggctgt tccggtcggt cacgacctcg
     1141 atcaggtacg gttcgtttag ccggatcgcc tccaggatcg cgggcttgat ttcgccggag
     1201 gcatgaatcc tcttgccttt caccccgtag gcttcggcga ttttcacgaa atcgggattg
     1261 taaggcttgc cgtctttctg gaagctggac cagacttccc gtccgtaatt gatcaattga
     1321 ccgtgccgtt caatggactg gccgaagttg ttgatcacca cccagatgac agggatcttg
     1381 tattccactg cggtggccag gacatagctg gacatgatga aggacccgtc gcccacgatt
     1441 gacaaagcga ctttgttggg ccgggccagc tttactccca tgaccgcgcc ggccgcccac
     1501 cccatctggg ccattcccga gttgttgaag aaggtgttgg gcgtgtggac cacggccaga
     1561 ccttcatagg cctgttgaat gtcccccgta tcggtgacaa tattggcatc ctttggcaaa
     1621 gcccgattca cttccacggc caggcggttg gtagagaccg ggtcttccgt cccctggtag
     1681 tccgattcga tcttggccag ccattcctct ctccacttct tcgtctgggc gacccagggc
     1741 tgccgggcct tctcctgttt tttccacagc ggtttcactg cttcgaggag ctgctgcagg
     1801 gcaatctttc cgtccgccag gattcccaca tcggcgaggc agcttcttcc gatgtcgagc
     1861 tggtcgatgt tgacctggat caatttggtc tccgggaaat tgtagaccac ccctttcctc
     1921 catccggagg tgtgggcgtc gcagaagcgg catccgatgg agagaaccac gtcggcggcc
     1981 tgggtcgcct tcaccgaact cccccagccg ctccggccgg ccggacccag gcagagggga
     2041 tgatcttcgg tgagggctcc cttgcccggc atggtagtca cgaccgggac ctgcagggta
     2101 tcgacgaact ctttcacctc aatccaggcc tcggcgatgt gagccccgcc gccgaccagg
     2161 agcaggggtt tctgagcctg ggcgaagagc ctagccgcct cctggatggc ctccgggttg
     2221 ccgcccgccc gggtggtgag gtggtctttc tcccaggtcg aggtgctgtc gatctccacc
     2281 tcgtgggagg aaacgtcaaa ggggatgtac acggccgtgg gtcccggcct tccggtgccg
     2341 gcgagcttat aggcctgcat gatctggtac gccagaatgt cgggccgcat ggcataccag
     2401 attctcttgg taatgggcct aaacatgttc ggggtgtcgt ctccgccatg tacgctaagt
     2461 tcctccaaac tgcccttatc caaataatgg gtgggaggac cggtggggat gatcaggacc
     2521 cccgaggagt ccagcatggc atttcccaga gccggcagga ggtgcaagac gccggggcca
     2581 acgctggcca tgaccggggc gaccttgtgt ctggcgcgaa aatacccgtc ggccatgtgg
     2641 acggcggtga tctcatgctt cggctggatc cctttgaggt gcggcttatc gatcagggcg
     2701 tccaggatgt tccagatgcc atgcccgttg tacccgaaat aatactcaac ccccagcttc
     2761 tcgaaacatt cggcaacaat ctgaccgcct ttcatcttgg ccatcttttt tctccttttt
     2821 tcttttcaac cccattgttt ctcatgtaac ataggaccaa tatttcccat atggcttata
     2881 tccagcgctg gaaattgtca agaagatatt tccggacagt cggatttctt cttgacaaaa
     2941 gattttcggc attctaaaaa cgcatagaat tttctcagcg acgaaaggag aggatcatga
     3001 aaaaactgat ttgggcgttg gccgtcggtt cttttttctt cggagccttt ctcccagcgg
     3061 gatggaccgc ggagccggtg aagatcggtg tgggattacc cctgaccggg cccctggcct
     3121 tcctggggac tgaatttctc aagggtgccc agctggccgc cgaggagatc aacaaatcgg
     3181 gcggtatcct ggggggccga aaaattgaat tggtggtccg ggaccacaag gggattcccg
     3241 ccgaggcggt gaccctggcc aagaggttga tcgccgaaga caaggtgtcg gtcatcgata
     3301 tcgacctgcc cagctccgcc aacatcgcgg tgcacgtggt gagcaaggag atgaaagtag
     3361 tgcagctcac cgggtttggt tttgccccgg atgtgaccga caaggggcat ccctatcact
     3421 tccgggtctg cacccgggcc gaatccctca cccgggtatt gggagagtat ttagcagctc
     3481 ttcccaacaa caagaagatc gccatggtag cccccaacga cgactacggc cggggcgacc
     3541 tcgcttcgat gatcgccgtc ttcaaaaaga tcgggaaccc ggaagtcgtt tatgaagcct
     3601 attacgagag gaaccagacg gacttcaaca ccatcctcct caagatgaaa tctttgaacc
     3661 cggactgcta ctacatcaac gtccgttggc cggccagcgc catcgtcctc cagcagatgg
     3721 aagacctggc catgctgaag ggaaaatacc tctcttcgag cgtcaacttt ttcaataagg
     3781 acctggccaa acgggtgggc cccctcatgg agggggcggt gatgacggtc tcctgggcgc
     3841 cagtgttcaa agacgaggaa tcccagaagc tggtgaaagc gtaccgggcc aagtacgggg
     3901 atgaccccaa cgactccgtc tccctgggct ggagcccgat catggtcgcg gccatgggga
     3961 ttaacaaagc ggggacggac aaagaccagg aaaaaatccg cctggcgata aaagggctca
     4021 agtacaactc cccccagggg cccatcaacg gttttgacga caaaggggat tccggagttc
     4081 ccgcttatgt gctgcagttc aagaacgggg agtatcacct gattaaataa agggttcttg
     4141 ggttccgggg ttcaaggggc tgaggaggcg aggagccaac gggggatggc ttctattcat
     4201 tcgatccctt gaatcctagg gtccttgcac cctaacccag cctagccgga accaaacggg
     4261 aatctgtagg ggcggccccc tgtggccgcc cgcaaaatgg gcaaccacgg ggggttgccc
     4321 ctacagtttt ttaccacctt gcggacaaat taacttttga gcacctaagg attttcctga
     4381 cgatgtggga agcgacgttc attctggcca ttcagcagat cgccagcggg ctggttatgg
     4441 ggtccatgta tgccctcatg tccatcggca tgatgctcat cctgggcatc atgacggtta
     4501 tcaacatggc ccacggcgag ttttacatgc tggggggata cgtctccttc ttcctgatga
     4561 cccagatggg gcttccttac ctggtctcgc tcccggtttc catggtggtc gtgggggcga
     4621 tcgggatcgc cagcgagaag atgaccatcc gtcccctggt cggccgtccc tggttttcca
     4681 cgttcctgac cacctttggc ctttccatga tcctgaggga tggagcccag atcatctggg
     4741 gggtggaccc ccgggaaatc gtatcccctt tttcccataa gcgggtcgtg ctgggaccca
     4801 ttttcctaac tgagcatcgc atcttcgtct tcgtggtcgg ggtagtggtg atcggcctcc
     4861 tctacctttt catcaagaag cataaagtcg gaatggccat gcgggccatc gtccgggacc
     4921 gggaggcctc ggcgctcatg ggcatcaacc tgaacgccat gaacagcttc accttcgccc
     4981 tgggggccgg aacggcggcc ctggccggcg ccctcctggg agccatcttc aacgtgcttc
     5041 cttccatggg ggaactgccg ctagtcaagg ggttcgccgt ggtcatcatg ggcggtatgg
     5101 gcaacgtcca ggggattctc ttcagcgggc tgatcctggg ggtggccgag agcctgtcgg
     5161 ccagcttcat ttctctcggc ctctcggatg ccatcgcctt tgccatcctg atcatcatcc
     5221 tgctgttcaa acccgccggc ctctttggga agagggtctg accatgaacc ggcggaagct
     5281 cctttgtctc tccggactcg ttctgatcct tctccttctg ccctggctcc tccaggccgc
     5341 ctacacccgg cacatcctgg tcatggccct gatcttcacc ttcagcgcca tggggctcaa
     5401 cgtgatcttc gggttcgccg ggcagaccgc cttcgggcag ccggccttct tcgccctggg
     5461 agcctacatc tcctctctcc tggccctgaa acttggattt cccttctggc tctgcctcct
     5521 gggcaccatg gtgctctccg ccgggttcgg gttcatgatc ggatatccct gcctgcaact
     5581 gcggggcatc tacttcggca tcaccaccat ggcctttgcc cagatcctgt actacatcgt
     5641 ttctaattgg gtggaactga cccgggggcc catgggcctg gtagacattc cgccggccag
     5701 cttcggattc ggcgatcatt ttcaattcgt cttcatcggc gaactgcgat actactacct
     5761 agtgctggcc gcttccctgg tcaccctgtt ctttctgtcc cggctcatgg agacccgcct
     5821 gggaaggggt ttcgtggcca tccgggaaaa ccaggacctg agcgcctcca tcggcattca
     5881 ccccttctgg gtcaagatga tcgccttcat gctggcctgc gtgatcaccg gcatgggggg
     5941 aagcttctac gcccactatg taaaattcgt gagcccgatc gagttccagt ggtatttcat
     6001 cggtctcacc ttcatcatgg tgatcaccgg cgggatcgga accatctggg ggcctttcat
     6061 cggtggggcg atttttacgg tcctccctga ggtcttccgc gccgtggaaa cctaccgcaa
     6121 tatcctagtg ggagcggtgc tcattctcat cgtgatcttc ttccccgaag ggatcatggg
     6181 gaaagtggcc caatggagag ggaaagacgc ccagcctgct ttggagcgga agaatgcttg
     6241 agacccaagg actgaccgta gccttcgggg gcctggtggc cgtgaaccag atcgacctct
     6301 ccgtctctcc aggggagatc ctgggcatga tcgggcccaa cggcgcgggg aaaaccacgt
     6361 tcttcaacct gctcaccgga ttcttgaccc ccacgtccgg ccggattctt ttcaagggaa
     6421 aagatattgc cgggctccct ccgagccgga tcgcctcttt ggggatctgt cggacctttc
     6481 agatcaccag cgtgtttcct ggcctcaccg tccgggaaaa cctcttgacc gggacctact
     6541 gctggacccg gacccttttt ttcgacaccc tcttccattc ccaagggtac tcccgccagg
     6601 gccaggagat ggagaagcat gcgggcgaga tcctggaatt tctccatatg gaggaccgca
     6661 aggacacggt ggcccggaac ctttcctacg gagaccagcg gcgcctggaa atcggaatcg
     6721 ccctggcttc ccgcagagag ttcctgctcc tggatgagcc ggctgcggga atgaacccgg
     6781 aagagacgca gaccctcagc cagctgatcc ggcagatccg ggataagggg atcaccattt
     6841 tcctgatcga acatcatatg cagctggtga tggggatctg cgaccggatc gcggtcctca
     6901 attacggccg aaagatcgcc gagggtaatc ccctggagat cagccgcaac ccccaggtca
     6961 tcgaggccta cctgggaaaa gaggaagaat ttgcttgagc tgaagtccat cgttgcccag
     7021 tacggccatg tgagagccct gaaggggatc agcttgacga tccaccaggg ggagaacgta
     7081 accctcctcg gggccaatgg ggcggggaag tccaccacgc tgaaatgcat ctccgggctc
     7141 ctgcggatct cctccgggtc catccttttt cagggagaga gaatcgatca tcttcctccc
     7201 cacaagatca tccgcgaagg aatcacccaa gtcatggaag gcaggcgcct cttccccgac
     7261 ctggatgtgc ttgagaattt acgcctggga gcctacctga gcgatgatcg ccaggcgatc
     7321 cagcagcgga tggaccgggt atttggttac tttcccatcc tgaaagaacg acaaaaacaa
     7381 ctggccagca ccttcagcgg cggagagcag cagatgctgg ccatggggcg gggactcatg
     7441 gcctctccga acctcctgat gttagacgag ccctcgctcg ggctggctcc catcctggtc
     7501 caggaggtgg ggaagattat ccgaacgatc aaccgagacg gggtgaccct tctgctcatc
     7561 gaacagaacg cccagatggc cctgcggctg gccaaccggg gatatgtgat ggagaccggc
     7621 tccattgccc tcgaagggac ttctcaggaa ctcatctcca atgacaaggt ccggaaggcc
     7681 tatatggggc tttgattttt cctccacatc ctttttgttc cgaattcggt accgcagctt
     7741 gccattcgcc cccacaaact ccgaacggat agtttggcga ttcccctgcc ttcggcattt
     7801 tcctttgaga ttcaccgccc tgaacccggg gtgtacaaat agcttggggt gggcagggtt
     7861 gagattcctt gcccaccccc agttcttaaa aaagagtcct tcgaaaataa gatttctgga
     7921 aaagaggggg gatctcatag ggttcagggc ggagaaattt ggatcatcat cctgaggcag
     7981 tggttcttcc attggatagg gcctgtcctg ggggggattg aaacggaaaa atgctttggc
     8041 tcttttttct gcttggagga atctcct
//
LOCUS       DASPRR010000305         4718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1845744, whole genome shotgun sequence.
ACCESSION   DASPRR010000305 DASPRR010000000
VERSION     DASPRR010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845744"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VEL68_11535"
     CDS             <1..914
                     /locus_tag="VEL68_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXZ36641.1"
                     /translation="LPVITSPREGLAEGAVKIHEKGNLLLHTKLRKGNVAEGFARADV
                     VVENTYKTQVVDHAYIETESGIGMLDKQDNLTIWSANQCPFRDRRQVAVVLGMRENKI
                     RIIRAATGGAFGGKDDVTVEVHLGLLARATRRPVRLVLDREESFLSQTKRHAIEIWTR
                     WGATKDGRLCAMEGDVWGDKGPYAGLGAFVVKKCGLHLSGPYYIPNIRVDSYSVYTNN
                     IFGSAMRGFGVSQAAIAHEAQMDELAKKLQINPLRMRLINCLKNGLSTATGQVFHEGV
                     GIEATLEKIREVALGDPSLHKYWEDLA"
     gene            911..1918
                     /locus_tag="VEL68_11540"
     CDS             911..1918
                     /locus_tag="VEL68_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXZ36642.1"
                     /translation="MKKRGIGVGSMFYGLGYGFSRQDIASATIEICEDGSVIVRSGEV
                     DFGQGSDTILCQITSEELGIPYNSIQMLTADTLTTPNAGPTSASRVTYVTGMAMLKAA
                     RAMKKTLHSAAAQILGQMDLVFGNDKVYSVSHPEKIISFKDLAKAAHLRGLPMVETAW
                     HDITTKDVDPQTAQGDAYSAYAYATQLAEVEVDTETGEVQVLRVVTATDAGKAINPMN
                     VEGQIEGGVAMGLGYALTEEIALEGGYLKTPSLGDYLIPTSLDVPPIETHIVEVPVST
                     GPYGAKGVGEPASIPTAPAIMNAIAAALGVRVAELPATPENLLRLIREKEREEKNLPP
                     K"
     gene            1933..2790
                     /locus_tag="VEL68_11545"
     CDS             1933..2790
                     /locus_tag="VEL68_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXZ36643.1"
                     /translation="MQHFQFYSAKSLNDALRFLAENGDQTRVLAGGTDLIPELREEIS
                     RPGMVLNILEIEGLNGVREANQGLRIGCTTTHAQLVESDILRRSCPSLVQAAGSIGGP
                     LIRNRGTIGGNIAHASPAADLASTLLSLDAEAVIMSQKGTREVALSDFFLGPGKTVLK
                     ADELLTEIAIPLPRGKGVFLKLGRRQAMTLSVVNVAVMLEMGEGRCREARIALGSVAP
                     TPIRCPGAERMLLSREMDEELIGKCAQEAMAVSKPVDDQRAKAWYRVQAGTVLVRRAL
                     ARATGLGEV"
     gene            2922..3404
                     /locus_tag="VEL68_11550"
     CDS             2922..3404
                     /locus_tag="VEL68_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ36644.1"
                     /translation="MPLKADKVPLKMVVNGKAVEVKVNPTWTLLKVLREELGLTGTKK
                     GCERGDCGACTVLLDGKPINSCLVLALQAEGKEVETIEGMGTAENLHPLQKSFIKNGA
                     VQCGFCTPGMLLSALALLRKNPNPTRQEIRRAISGNLCRCTGYAKIVKAIQDVSAIQG
                     "
     gene            3718..>4718
                     /locus_tag="VEL68_11555"
     CDS             3718..>4718
                     /locus_tag="VEL68_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ36645.1"
                     /translation="MGQRLNKTSALLITAAGSFLMPFMGSSVNVALPSIGREFSMDAI
                     LLSWVSTGYLLAAAMFLVPFGRIADIHGRKKIFSSGILVFTLCSLFLGLSTSAHTVLV
                     LRVLQGIGGAMTFGTGVAILTSIFAAGERGKALGFNVTAVYMGLSLGPFLGGFLTEHW
                     GWRSIFLVMVPLGVAVLGLIHWKLEGEWAEARGERFDYMGSSLYGLSLVAVMVGFSQL
                     PQWLGEELILGGLVGLGAFVRWEMKIPSPVLNISLFRHNRVFALSNLAALIHYSATFG
                     VGFLLSLYLQYIKGLSPSHAGSILLCQPVVQALFSPFAGRLSDRIEPRVVSSLGMGFS
                     "
BASE COUNT          960 a         1359 c         1446 g          953 t
ORIGIN      
        1 cccttccggt gatcactagc ccgcgggaag gactggccga aggggcggtc aaaatccacg
       61 aaaaaggaaa tctccttctc cacacaaagc tccgcaaagg gaacgttgcc gagggattcg
      121 cccgcgcgga cgtggtggtg gagaatacct acaaaaccca ggttgtggac cacgcctaca
      181 tcgagaccga gtcgggaatc ggcatgctgg acaagcagga caacctcacc atctggtcgg
      241 ccaaccagtg tcccttccgg gaccggcggc aggtggccgt cgtcctgggg atgcgggaga
      301 ataaaatccg gatcatccgg gcggcgaccg gaggagcttt cggcgggaag gatgacgtga
      361 ccgtggaagt ccacctcggc cttctggcca gagccacccg gcgcccggtg cggctggtcc
      421 tggaccggga ggagtccttc ctctcccaga ccaagcgcca cgccatcgag atttggaccc
      481 ggtggggggc tacgaaggac ggcagactct gcgccatgga gggggacgtc tggggagaca
      541 agggccccta tgccgggctg ggggccttcg tggtcaagaa atgcggcctt cacctgagcg
      601 ggccctatta tatccccaac atccgcgtgg attcgtacag cgtctacacc aacaacatct
      661 tcggaagcgc catgcgcggc ttcggcgtct cccaggctgc catcgcccat gaggcccaga
      721 tggacgagct ggccaagaag ctccagatca accccctgcg catgaggctg atcaactgcc
      781 tcaagaacgg cctctccaca gccacgggcc aggtctttca cgaaggggtg ggcatcgagg
      841 ctaccctgga aaaaatcagg gaagtcgctc ttggagatcc ctcccttcac aaatactggg
      901 aggacctggc atgaagaagc gcggcatcgg tgtgggttcc atgttttacg gcctgggata
      961 cgggttctcc cggcaggaca tcgcttcagc cacgatcgag atttgcgaag acggctcggt
     1021 catcgtccgg tccggagagg tggacttcgg gcaggggtcc gatacgatcc tctgccagat
     1081 cacgtccgaa gaactgggta tcccctacaa ctccatccag atgctcaccg ccgatactct
     1141 cacgaccccg aatgctgggc ccacttcggc tagcagggtc acttacgtaa ccggtatggc
     1201 catgctgaaa gcggcccggg ccatgaaaaa gacccttcat tccgcagccg cgcagattct
     1261 gggacagatg gaccttgtct tcgggaatga caaggtctat tccgtttccc acccggagaa
     1321 aatcatctcc ttcaaggacc tggccaaggc ggcccatctg cgtgggcttc ccatggtgga
     1381 gaccgcctgg catgacatta ccaccaagga cgtggacccc cagacggcgc agggagacgc
     1441 ttattcggcc tatgcctacg ccacccagct tgccgaagtg gaggtggata cggaaaccgg
     1501 agaggtccag gtgctccggg tcgtcacggc cactgacgcc ggcaaggcca tcaatccgat
     1561 gaacgtggag gggcagattg aaggcggagt ggcgatgggg ttgggatatg cgttgaccga
     1621 ggagatcgcc ctggaagggg gctacctgaa aacgccttcg ctcggggatt acctgatccc
     1681 gaccagcctg gatgtgccgc cgatagagac ccatatcgta gaagtgcccg tgtccacggg
     1741 gccctatgga gccaagggtg ttggggagcc ggcttccatt cccacggctc cggcgattat
     1801 gaatgccatt gccgctgcgc tgggagtgag ggtcgcggaa cttcctgcga cccctgaaaa
     1861 cctgctgcgc ctgatccggg aaaaagagcg ggaagagaag aacctccctc ccaagtagga
     1921 aagaaaggcc caatgcaaca ttttcagttc tattctgcca agtccctgaa cgatgccctg
     1981 cgctttcttg cagaaaacgg ggaccaaacc agggtcctgg ccgggggaac ggacttgatc
     2041 ccggagttgc gggaggagat ttcccgcccc ggcatggtgc tgaatattct ggaaatagag
     2101 ggactgaatg gcgtcaggga ggccaaccag ggcttacgca ttggctgcac aaccacccat
     2161 gcccagttgg tcgaatccga tatcctcagg aggtcttgcc cttccctggt gcaagcggcc
     2221 ggatcgattg ggggacctct catccggaac cgggggacga tcgggggaaa tatcgcccac
     2281 gcctctccgg ctgcggacct tgcctccacg ctcctgtccc tggacgccga agcggtgatc
     2341 atgagccaaa agggaacccg ggaggtcgcc ctcagcgatt tcttcctcgg ccccgggaag
     2401 actgttctaa aggcggacga gctcctgacg gagattgcga ttccgttgcc ccggggcaag
     2461 ggagtgttct tgaaactggg gcgccgccag gccatgaccc tttcggtcgt gaacgtggca
     2521 gtcatgctgg agatggggga ggggagatgt cgggaagcca ggatcgcttt gggttccgtc
     2581 gctccaactc caatccgatg ccccggggcc gaacggatgc tcttgagccg ggaaatggat
     2641 gaagaattga ttggcaaatg cgcgcaggaa gccatggcgg taagcaaacc cgtggatgat
     2701 cagcgcgcca aggcctggta ccgggtccag gcgggaaccg tcctcgtaag gagggccctc
     2761 gcccgagcta ctggcctggg agaggtttga gcacgggagg ggaagtgctt cacggtgacg
     2821 catctcccgc cgggttttgg cggcatgaat ttgcccctcc tccggacaac gagagaagtt
     2881 tttcctagaa aaaaggccgt ctgaaaaaaa agaggatctt catgccgctg aaggccgata
     2941 aagtcccgtt gaagatggtc gtaaacggca aagccgtaga ggtgaaggtc aaccccacct
     3001 ggaccctcct taaagtgttg cgggaggaac ttggattgac ggggaccaag aaaggctgcg
     3061 agaggggaga ttgcggggcc tgcaccgtgc tcctggacgg gaaaccgatc aattcctgtc
     3121 tcgtcctggc cttgcaggcc gagggaaagg aggttgaaac catcgaaggg atgggcacgg
     3181 ccgaaaatct tcatcccctg caaaagagct tcatcaagaa cggggcggtg caatgcggct
     3241 tctgcacgcc gggaatgctg ctctccgccc tggctctcct gcgcaagaac cccaacccga
     3301 cccggcaaga aatccggcga gcgatctccg gcaacctgtg ccggtgtacc ggctatgcga
     3361 agatcgtcaa ggccattcag gacgtttccg ccatccaggg ctaaagagag aagttcggca
     3421 gatcgatatc gattacgatt taaagctcta tcagaccctc tttcttcatg gtcagcgcgg
     3481 tccaggttca agttcgggcc ctttggctat atttccccac cgccgcgaga tgcgcacccc
     3541 ggaagcgaaa atccccagaa acggttttcc cggtagcatt cactttccgc cggggtggct
     3601 tgaatcgcag tgatccctag cctggctacg tcttctgccc gggctttttt agggcggcgt
     3661 agagagcttt ctcgaaaaat tagaagacgg ccgtacaaaa tcagctagaa taataccatg
     3721 ggacaaaggc tgaataagac ttctgcgctt ttgattaccg ccgcgggttc ttttctgatg
     3781 cccttcatgg gctcctcggt caacgtggcc ctgccttcca tcggcaggga attttccatg
     3841 gatgccatcc tgctcagctg ggtgtccaca ggctatctcc tggctgcggc catgtttctg
     3901 gtccccttcg ggaggatcgc ggacatccac ggaaggaaaa agatcttttc ttccggcatt
     3961 ctggttttca ccctctgttc cctcttcctg ggcttatcca cgtcagccca tacggttctc
     4021 gtcctgcgcg ttttgcaggg aatcgggggg gcgatgacct tcggcaccgg ggtcgccatc
     4081 ctcacctcta ttttcgcggc gggggaaagg gggaaggctc tggggttcaa cgtgaccgct
     4141 gtttatatgg gcctgtccct ggggcctttc ctggggggat tcctgaccga gcattgggga
     4201 tggaggagta tttttctggt catggtcccc ctcggggttg cggtcctggg cctcatccat
     4261 tggaaattgg aaggggaatg ggcggaggcc cgaggagaaa ggtttgatta tatggggtcc
     4321 tccctctacg gtttgtccct ggtggcggtc atggtcggtt tttcccagct gccccaatgg
     4381 ctgggggagg aactcattct gggcgggctc gtaggcttgg gggctttcgt cagatgggag
     4441 atgaaaatcc cgagtccggt tttgaacatc agcctcttcc ggcataaccg ggtctttgct
     4501 ctctccaatc tggcggcgct gatccattac agtgccacct ttggggtcgg cttccttctg
     4561 agcctgtacc tgcaatacat caaagggctc agtccctccc atgccggttc cattctcctt
     4621 tgccagcccg tcgtccaggc ccttttttcc cctttcgccg ggcgtctctc cgaccggatc
     4681 gaaccccggg tcgtctcttc cctgggaatg ggattttc
//
LOCUS       DASPRR010000306         6255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_387255, whole genome shotgun sequence.
ACCESSION   DASPRR010000306 DASPRR010000000
VERSION     DASPRR010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387255"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VEL68_11560"
     CDS             complement(<1..368)
                     /locus_tag="VEL68_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ36646.1"
                     /translation="MKMDVVISGVGGQGNIFASLVISQYAMNKNLKVLGAETIGAAQR
                     GGAVVSHIRMSDGEIYSPIISRGQADLLIGMEYVELMRHMRLLNPEGMYLLNAMKIPT
                     VLNNMALDEYPATEDILKAIR"
     gene            complement(365..2200)
                     /locus_tag="VEL68_11565"
     CDS             complement(365..2200)
                     /locus_tag="VEL68_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ36647.1"
                     /translation="MKEKKYVMGNTAIARGALEAGVRVVAGYPGTPATEIVDECLGVP
                     GVHVEWANNEKVAFELCTGASLCNVRALPVMKHNGTNYVTDFLMHVNFTGIRAGLVLI
                     SADDPGGLSSQNEEDTRILVHQYAHLPIFDPSGPSEAKAMIKEAFELSEKTELVFCLR
                     PVTRVCHARGIIEFDDLPKDLPPAHYEPDRERFIMSAVEMPQFGGIKRPQIRHRWLNE
                     KQVLLEKIMEESLYNWIEEGQGKVGLIGCGMGYTWIKEAEQFMGAKLPLLKLGTLPLP
                     RKKLLEFVEGLNKVVVFEETEPFVESLCKKIFQEEKIRVEVLGRSGFLPSDGELSLQA
                     VLDGVKKAAPKVKVRKPETPAMDIHIPIRTRTQCVGCNYRGILNALKQTVRKHKGVVA
                     GDIGCHDAGSFRPLELQSTIYCMGSSIPMASGMALSGVNRPVFTFIGDSTFYHNGILG
                     LINAANNKINVNVILGENTVTAMTGFQPHPGTGMTLAGEKTTPVNVPKLAESMGVSYR
                     VVNPYDIKQCREALEAAVKEEGPSLIVSSMPCFLLSTRQGKKVFDPREVKVDLEKCTG
                     CMICINDFGCPAISFDKVKKKVSIDPMICVGCGLCVDVCQKGAIV"
     gene            2515..3201
                     /locus_tag="VEL68_11570"
     CDS             2515..3201
                     /locus_tag="VEL68_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXZ36648.1"
                     /translation="MKIEMIEARDLPDAWFQCVYRILDSGYEYKIDHGSFAGQKRREF
                     DFVVVHIKFPGVRPLIPDIPPGLGIPAPTTMEYVDQYMRYWITDERQEGETYTYGERL
                     VNPKVNEVPLGVNPVEEAIKIYKTGGPGTNQATMEVGMPQDIVNPDPPCLRLIDTRVR
                     YGKLHFVVYFRSWDLWAGFPANLAGIQQLKEYMAAQIGVEDGEIIAVSKGMHLYDYTW
                     DLAKLRTYRT"
     gene            3373..3972
                     /locus_tag="VEL68_11575"
     CDS             3373..3972
                     /locus_tag="VEL68_11575"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXZ36649.1"
                     /translation="MEKKIKEILACREKACIDEPGLIRAAVLIPLFKKQGEYHVLLTR
                     RTDKVGTHKGQISFPGGKRDEGEDLLCTALREAREEMGIAEKDVRILGELDDMCTIAS
                     DFCIAPFVGLIPYPYPFKINRHEIEEIIEVPLSVFLDETKFREELRSRNGELIPVFFY
                     QHDEHTIWGATARILKQLMDLISQEDRKSESRPGSKNGF"
     gene            4059..4544
                     /locus_tag="VEL68_11580"
     CDS             4059..4544
                     /locus_tag="VEL68_11580"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HXZ36650.1"
                     /translation="MMGDTSEFSLTIRLFLKAYQWRKIDPVPWTPLKKPLSQSKLALV
                     SSAGFVLPDQPHFDESIKGGDPSFREIPSGVEVKTLVETHRSESFDHTGLQKDPNVGF
                     PIDRVRELAVSGRIGAVNHRHLSCMGSITAPGRLIHDTAPPAVHLLVEDQVDVALLVP
                     V"
     gene            4548..4814
                     /locus_tag="VEL68_11585"
     CDS             4548..4814
                     /locus_tag="VEL68_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36651.1"
                     /translation="MCNQTVCLVAAELERQGISTVVIQLLRNVAEKIRPPRALFVPFK
                     HGYPLDMPGDSGRQKAVIAAALQLLEDPSLKAGPVLRDYTPPQR"
     gene            4879..5853
                     /locus_tag="VEL68_11590"
     CDS             4879..5853
                     /locus_tag="VEL68_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXZ36652.1"
                     /translation="MRKRSLAVFTLPLLAAFVLVATTQAADKYKGYARGEIFITAQEL
                     NRLIQAKDPKLVVIAVASKTEYYSGHIPGSFHLWRPDYEADPKTQGGVTDNLIEPEGY
                     TKLMQKIGVDPDSKVVVYDHKYDATRLWWAFYYYGKTDVRILDGGIKAWTRAGFPTDM
                     VAGGKAAKAGTWVAKVTSPKMRVDTPEVLGTKDRADAQLWDVREDKEFCGDTIKEGAH
                     RAGRIPWGVQADYVLVKTKENHSEWQSAGDVQKNMEKLGFDPQKQQYFYCQSGVRCTN
                     WISTLYAMGWPMEKLHNYDGSWIAWSLDQKLPIEIGCPDTTPAQWQKK"
     gene            5949..>6255
                     /locus_tag="VEL68_11595"
     CDS             5949..>6255
                     /locus_tag="VEL68_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36653.1"
                     /translation="MEVHGQQGKPAGVEKRGKPWLRLLIGAGLIVVLLLVAYQAGLLD
                     LIKGGTLKERVERLDGMFQSLGAWAPAFFILIWVFACALLLPGLPISLVGGLVFGAVW
                     "
BASE COUNT         1364 a         1783 c         1791 g         1317 t
ORIGIN      
        1 cggatggcct ttaggatatc ctcggtggct gggtattcat ccagtgccat gttgttcagc
       61 actgtgggga tcttcatggc gttcaggaga tacatcccct cggggttcag caggcgcatg
      121 tgccgcatga gctccacata ctccatcccg atcaggagat cggcctgccc ccgggagatg
      181 atgggggagt aaatctctcc gtcggacatg cgaatgtggg agaccactgc cccgccccgc
      241 tgggccgcac cgatggtctc cgcccccagg actttcaggt tcttgttcat ggcatattga
      301 gaaatgacta gggaggcaaa gatatttcct tgtcctccca ccccggatat gaccacatcc
      361 attttcatac gatggccccc ttctggcata catctacaca gagtccgcac cccacgcaga
      421 tcatgggatc gatagaaacc ttcttcttca ctttgtcgaa ggagatggcc ggacagccga
      481 agtcgttgat gcagatcatg cagccggtgc atttctctag gtcgaccttc acctcccgcg
      541 ggtcaaaaac ctttttccct tgccgggtgg agaggagaaa gcagggcatg gaggagacaa
      601 tcagcgaagg accctcttct ttgactgccg cctctagggc ctctcggcat tgcttgatat
      661 cgtaggggtt aaccacccgg tagctgactc ccatggattc tgccagcttg ggaacattga
      721 ccggagtggt cttctctccg gcaagcgtca tccccgttcc tgggtggggc tggaacccgg
      781 tcatggccgt gaccgtattc tcccccaaga tcacgttgac gttgatctta ttgttggccg
      841 cgttgatcag ccccaggatg ccgttgtggt agaaggtgga atcaccgatg aaggtgaaga
      901 ccggccggtt cacgcccgaa agggccattc cactggccat ggggatcgaa gaacccatac
      961 aataaatcgt agactggagt tccagagggc ggaacgaacc ggcgtcgtgg cagccgatgt
     1021 cgccggccac gacccctttg tgcttgcgaa cggtctgttt tagggcgttg aggatgcctc
     1081 ggtagttgca gcctacgcac tgggtacgcg tccggatggg aatgtggata tccattgccg
     1141 gagtctccgg cttgcggacc ttgaccttgg gagccgcttt cttcaccccg tccaggacgg
     1201 cctgaaggct gagttccccg tccgatggca ggaagccgct tcgcccaagg acctccactc
     1261 tgattttttc ttcctggaag atttttttgc acaggctttc cacgaaaggt tcggtctcct
     1321 cgaagaccac caccttgttc aacccctcga cgaactcgag gagcttcttc cgggggagcg
     1381 ggagggttcc cagcttcaag aggggaagct tggcgcccat aaactgctcc gcttccttga
     1441 tccaggtgta gcccatgccg cagccgatca aaccgacctt tccctgcccc tcctcgatcc
     1501 agttatacag gctctcttcc atgatcttct ccaggaggac ctgtttttcg ttcagccagc
     1561 ggtgccggat ctggggtctt ttgatccccc cgaactgggg catctccacc gcactcatga
     1621 tgaagcgctc ccggtccggc tcatagtgcg ccggggggag gtctttgggc agatcgtcga
     1681 attcgataat cccccgggca tggcaaaccc gggtcaccgg gcgcaggcag aagaccagtt
     1741 cggtcttctc cgaaagctcg aaggcctcct tgatcatcgc cttggcctca ctggggccac
     1801 tgggatcaaa gatggggaga tgggcgtact ggtggaccag gatgcgggta tcttcctcgt
     1861 tctgggagga cagtccgccc gggtcatcgg ccgaaatcag caccagtccc gcccgaattc
     1921 cggtgaaatt gacgtgcatt agaaagtcgg tcacataatt ggtgccgttg tgcttcatga
     1981 ccggcagggc gcggacattg cagagagagg ctccggtgca gagctcgaag gctaccttct
     2041 cgttgttggc ccactccacg tgaacccccg ggactcccag acactcatcg acgatctccg
     2101 tggccggagt cccgggatag ccggcgacca cccgaacccc ggcttctaga gccccccggg
     2161 cgatggccgt atttcccatg acgtactttt tctctttcat gtttccttct ctcctgattt
     2221 tctgacttct tcggggtgaa aagacttccc ttttcaccgc ctagcgaaaa aaatggcagg
     2281 caatggagga tacctatttc tatcattgag agaggggtaa ggcaagaagg aaaagatttt
     2341 aaaatgcagg gtacggaatg tgaaatgcgg aattcacaag ggaaacctat ttcattgctt
     2401 gggattttta ttcccctctc gagaggttcc catttgggag gttgaattgg caaacctttc
     2461 atgccatatt gaataataag gttaaggcga aaaattcttg tatgaggttg atcgatgaag
     2521 atagagatga tcgaggccag agacctcccc gatgcctggt tccagtgcgt ttaccggatc
     2581 ctggattctg gctacgagta caagatcgac catgggagct ttgccggaca gaagcgccgg
     2641 gagttcgact tcgtggtggt ccacatcaag ttccccgggg tccggccgtt gatcccggac
     2701 atccccccgg ggctgggaat tcccgctccc accaccatgg agtacgtgga tcagtacatg
     2761 cggtactgga tcaccgatga gcgccaggaa ggagaaacct atacctacgg ggagcgtttg
     2821 gtcaacccca aagtcaatga ggtccccctc ggggtgaacc ccgtcgaaga ggcgattaag
     2881 atctataaga cgggcggtcc aggaaccaac caggctacca tggaagtggg catgccccag
     2941 gacatcgtga atcctgaccc cccctgcctg cgcctgatcg acacgcgggt tcgttatggg
     3001 aaactgcact tcgtcgtcta tttccgatcc tgggacctct gggccggttt ccctgctaac
     3061 ctggccggaa tccagcagct gaaagaatac atggctgccc aaatcggagt tgaggatgga
     3121 gagatcatcg ccgtctccaa aggcatgcac ctctacgatt acacctggga cctggccaaa
     3181 ctgcgcacct accggacctg attttttcat cacagaggcc cagagttcac ggagaaagaa
     3241 aacaatataa ctagggtgaa acaaccaaac gtcgaaacag ggggaaaagg actggggcct
     3301 tccctatatt cctcgattca aattttcatt tccttactct gtgttctctg tgcctctgta
     3361 gtgaactaaa aaatggaaaa aaagatcaaa gagattctgg cctgtcggga aaaggcctgt
     3421 atcgacgagc ctgggttgat tcgggcggcg gtgctcatcc ccctcttcaa gaagcagggg
     3481 gaataccacg ttctcctgac ccggcggacc gataaggtgg gaacccacaa gggccagatt
     3541 tctttccccg gggggaagcg ggatgaaggt gaagacctcc tctgcactgc cctgcgcgaa
     3601 gcaagggaag aaatggggat cgcagaaaag gatgtacgca tcctgggcga gttggacgac
     3661 atgtgcacga ttgcctccga tttctgcatc gcccccttcg tgggtctgat tccctacccc
     3721 tacccgttca aaatcaaccg ccacgagatc gaggagatca tcgaggttcc cctctcggtg
     3781 tttctggacg agaccaagtt ccgggaggag ctccgtagtc gcaatggaga gctaattccc
     3841 gtctttttct accagcacga tgagcacacc atctggggcg ccactgcccg gattctgaaa
     3901 cagttgatgg acctcatatc ccaagaggat cgaaagagcg aatcgagacc cggctctaaa
     3961 aatggctttt aggggcgcct tgccgggcgc ccgttaagga atcgaaaccg gggttccgga
     4021 agcacaagtt agtttaaggc ccttgaccaa cgaggcgaat gatgggcgat acgagcgagt
     4081 tttcactgac tatccgtctg ttcctgaagg cttatcaatg gcggaagatc gatccggttc
     4141 cgtggacgcc cctcaagaaa cccctgtccc agagcaaact ggcgctggtg tccagcgccg
     4201 gattcgtgct cccggaccag ccccatttcg atgaatcgat taagggcgga gacccgagct
     4261 ttcgggaaat cccctccggg gtcgaggtga agaccctggt ggaaacccac cgcagcgagt
     4321 cttttgacca caccggcctt caaaaggacc ccaacgtggg cttccccatc gaccgggtcc
     4381 gtgaactggc cgtttcgggg cgcatcggcg cggtgaacca tcgccatctt tcctgcatgg
     4441 gatcgatcac cgcaccgggc cgtttgatcc acgacaccgc tccgcccgcc gttcatctgc
     4501 tggtggaaga tcaggtcgac gtggctctgc tggtgccggt ctgacccatg tgcaaccaga
     4561 ccgtgtgtct ggtcgccgcc gagctggaac gccaggggat ttccaccgtg gtcatccagt
     4621 tgctccgcaa tgtggctgaa aaaatccggc cccctcgggc cctgttcgtt cccttcaagc
     4681 acggttaccc cctcgacatg ccgggggact ccggccgcca gaaggcggtg attgcggcgg
     4741 cccttcagct tctggaagac ccctccctaa aggcggggcc ggtactaagg gattatacgc
     4801 ctccccagag ataaaacccg gagaaggaca ttgctgtccg tgcctgcatg cagaataagc
     4861 ctatgaggag ggaaagatat gagaaaaagg agcttggctg ttttcaccct gcccctgctg
     4921 gccgcttttg ttttggtcgc gacaacccag gctgcggata agtataaagg ctatgcgcgg
     4981 ggagaaatat tcatcaccgc ccaggaactc aaccggctca tccaggccaa ggaccccaag
     5041 ctggtggtca tcgccgtggc cagtaaaact gagtattact ccggacacat tccggggtcc
     5101 ttccacctct ggcgtcccga ctatgaggcc gacccgaaaa cccagggggg ggtgaccgac
     5161 aacctcatcg aaccggaagg atacaccaag ctgatgcaaa aaatcggcgt ggacccggac
     5221 tccaaggtcg tggtgtacga ccacaagtac gatgccaccc gcctttggtg ggcgttctac
     5281 tattacggaa agactgacgt tcgaatcctt gacggcggga tcaaggcatg gacccgggcg
     5341 ggattcccga ccgacatggt agccggcggc aaagccgcaa aggcaggaac ctgggtggcc
     5401 aaggtgactt ccccgaagat gcgggtggat accccagagg tcttgggcac gaaggaccgt
     5461 gccgacgccc agctctggga cgttcgggaa gacaaggaat tctgtggtga cacgatcaag
     5521 gaaggagccc accgcgcggg acggatcccc tggggcgtgc aggccgatta tgttctggtc
     5581 aagaccaaag agaaccattc cgagtggcaa tccgcaggcg acgtgcaaaa gaacatggag
     5641 aagcttggct tcgatcccca aaaacaacag tacttctact gccaatcggg agtccgttgc
     5701 acgaactgga tctccacgct ttacgccatg ggctggccaa tggagaaact tcacaactat
     5761 gacggctcct ggatcgcctg gtccctggac caaaaacttc ccatcgaaat aggctgcccg
     5821 gacacgaccc cggcacaatg gcagaaaaag taattccggc agactggaat gttctgccct
     5881 ccgcagatca gcccaagttt cttcaggtgg gatccaggac ggaaaagcgg gccggtaggg
     5941 gaggaagaat ggaagtgcac gggcaacaag gaaagccagc cggggtagag aagcgaggca
     6001 aaccctggct gcgcctgctt atcggcgccg ggctcatcgt ggttctcctg ctggttgcct
     6061 accaggcggg gctcttagac ctgatcaagg gaggtacgct caaggaaagg gtggagcggc
     6121 tggacggaat gttccagtcc ctcggcgcct gggcgcctgc tttcttcatc ctgatctggg
     6181 tcttcgcctg cgcacttctg cttccgggac ttcccatctc cctggtggga ggcctggtgt
     6241 tcggagccgt gtggg
//
LOCUS       DASPRR010000307         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_877141, whole genome shotgun sequence.
ACCESSION   DASPRR010000307 DASPRR010000000
VERSION     DASPRR010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877141"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VEL68_11600"
     CDS             complement(<1..454)
                     /locus_tag="VEL68_11600"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXZ36654.1"
                     /translation="MKSNLKAPTPQNIILIGMPGVGKSTVGVLLAKALSRDFLDTDVY
                     IQAKERRTLQEIIDQKGLRAFCQIEERHLRSLKFRSSIIATGGSVVYYPGAMARLRSS
                     GIVIHLDLALAPLKKRLTNLPTRGVVMVPGQSLDQLFDERLPLYRKYSH"
     gene            999..1769
                     /locus_tag="VEL68_11605"
     CDS             999..1769
                     /locus_tag="VEL68_11605"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXZ36655.1"
                     /translation="MKDLFSLKGKVAIVTGGNGGLGLGIARGFASVGGDIVIAARNAS
                     KSAKAGEGIRKDFGVKVLEVDVDVRQEKQIQGLVKKVLDTFGRIDVLVNNAGINIRKF
                     PQDLLSSEYDEVLEVNLRSAYLTCKYVYPAMKKVGGGKVINIGSMTSIFGGGKLIAYG
                     TSKGGIVAMTRAMAVGWATDNIQVNAILPGWIDTDMTRRARVELEGLNEKVLARTPVG
                     RWGTPEDLAGTAIYLATAASNFITGVALPVDGGYSVSI"
     gene            1929..2438
                     /locus_tag="VEL68_11610"
     CDS             1929..2438
                     /locus_tag="VEL68_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXZ36656.1"
                     /translation="MQRCYQVSRELLKFLNDQEPFGLSRDVSLTWERIHSMGSARIAA
                     LLAAKRGVDVELSYAAGALHDFGRFVTGKQENHAENGYEPAKDFLRSLNIFSEGEREE
                     IARAVKNHSRKEIVGTPLEEIAKDCDILDCYYLGLQPKKEAHLKRFGALQKELGLGLE
                     ENLRIPTPG"
     gene            complement(2496..3314)
                     /locus_tag="VEL68_11615"
     CDS             complement(2496..3314)
                     /locus_tag="VEL68_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXZ36657.1"
                     /translation="MGRKIISLAMFLAFLLLFLNAPAGPQVYRYKDKNGVVHFTDTPP
                     DDKFTRVQNERFRSTQFPYYGNNLLIEMASNRLELVSRAVVGVKTNSTRGSAFLINPL
                     GHAVTNYHVIAEAGGDIQAVTFDGQEWNAWVISTDPDKDLALIHIGSGGYPYLPLGKL
                     TDVSIGKEVYIVGNPLGLSHSMSKGIVSSVRKGMNKNTPLTLIQTDAAINAGNSGGPL
                     ITPEGLVLGVVTFKAGTPNLPVQGIGFAVSSEDIISGLSLTPAKESPGPNSSKE"
BASE COUNT          870 a          943 c         1055 g          861 t
ORIGIN      
        1 gatgggagta tttccggtaa aggggaagcc tttcatcgaa taattgatcg agactttggc
       61 cggggaccat caccactccc cgggtgggca ggttggtcag cctcttcttt agaggggcca
      121 gagccaggtc cagatggatg acgatgcccg aggaccgaag acgggccatg gctccgggat
      181 aataaaccac actgcccccg gtagcgatga tggatgaacg gaatttcaag gagcgcaagt
      241 ggcgctcttc gatttggcaa aaggctctta accctttttg gtcgatgatt tcctggaggg
      301 tccgcctctc tttggcctga atgtaaacat ccgtgtccag aaaatcgcgg gatagagcct
      361 tggccaagag taccccgacc gtgctctttc ctaccccggg catgccgatg aggattatat
      421 tttggggagt cggggctttg aggtttgatt tcatttcccg gagaaaaaat aggacatttt
      481 tcttctttta ttggctcttc tcccattcga gtgggtgatt ttggtctatt tcccagctgc
      541 ccatgtaatc tctgccagca ggcaggaatt ggcccaggcc cagggggcgc atcccgtcca
      601 tggcaggaca taatgaggcg gccgtccttt catcggttcg ggtcggaaaa ttctttggtt
      661 ggtcgggtcc taccagggtt cggttttccg caaggggatc ggggacaggt gccttgcgga
      721 actccaggga ccgcttcgga atccaagctt tttcccgccg attctgtcct gccatggacg
      781 ggatgcgccc cctgggcctg tgagggagca ctttttgagt tatgtttttc cccactcgat
      841 tgagaaaaaa atccttttat tattatagat gaaggaaatc ccgtcgtaaa cggctagacc
      901 ggccaacaaa tcgcttgaaa acccaacccc ggcagggata tagtttctta gaactggttt
      961 gagatttcaa aagccaaaga gcgaatagga ggagcgcgat gaaagatctt ttcagtttga
     1021 aagggaaagt ggccatcgtc actgggggga acggcggcct cggcctggga atcgcccgcg
     1081 ggttcgcgag cgtgggaggg gacattgtca ttgccgcgcg gaatgcgagc aaatccgcca
     1141 aggccggcga ggggatccgc aaggattttg gggtgaaagt gctggaggtc gacgtggatg
     1201 tgcggcagga gaaacagatc caaggcctgg tgaaaaaagt cctggatacc ttcggccgga
     1261 tcgacgttct ggtgaacaac gctggaataa acatccgaaa attcccccag gacctcctga
     1321 gctcggaata cgacgaggtt cttgaagtca acctgcgtag cgcctattta acctgcaaat
     1381 acgtttaccc ggccatgaaa aaggtgggtg gaggaaaggt cattaacatc ggctccatga
     1441 cctccatctt cggaggggga aaactcatcg cctacggcac gagcaaaggg gggatcgtgg
     1501 ccatgactcg ggccatggcc gtgggttggg ccaccgataa catccaggtg aacgccattc
     1561 ttcccggctg gatcgatacg gatatgaccc ggagagcgcg ggtcgagttg gaaggattga
     1621 acgaaaaagt cctggcccgc actcctgtcg gtcgctgggg gactccggag gacctggcgg
     1681 gaacggcgat ctacctggcc accgccgcct ccaatttcat caccggcgtc gccttgccgg
     1741 tggacggcgg gtactcggtg agcatctaaa aagtcctttc cttttaccat gaggtagggg
     1801 caattcatga attgccccta caggaaaata atcaaggagc taccgtaggg gcgggtttgg
     1861 aacccgctcc taccgatctt accccgaaag atttctgatt caagggggag atcccgtaga
     1921 gtctttccat gcagcgttgt taccaggttt ccagagagtt actgaaattt ctgaacgatc
     1981 aggaaccgtt cggcctgagc cgcgatgttt ccctgacctg ggagcggatt cacagcatgg
     2041 gttcggcgag aatcgccgcc cttttggcgg cgaagagggg agtcgacgta gagctgagtt
     2101 atgccgccgg tgccttgcac gatttcggga gattcgtgac cgggaagcag gaaaatcatg
     2161 cggaaaatgg atatgaaccg gctaaggatt ttctccgaag tctgaatatt ttttcggaag
     2221 gagaaagaga agaaatagcc agggcagtga agaatcatag ccggaaggaa atcgtgggaa
     2281 ctcccctgga ggaaattgcc aaggattgcg acattttgga ttgctactac ttaggactcc
     2341 agcccaaaaa agaagcccat ctcaaacgct tcggcgccct gcaaaaagag ctagggctcg
     2401 gcctggaaga aaacctccga attccgaccc ccggttaact cttgactgcc gcagcccctt
     2461 ggagcctttc accagaaacc ctttcgaccg gatccttatt ccttcgatga gttgggaccg
     2521 gggctctctt ttgccggggt caggctcaaa ccgcttatga tatcctcgct ggaaaccgca
     2581 aaccctatgc cctgaacggg aagattcgga gtgccggctt taaaggtgac cactcccagg
     2641 accagaccct cgggggtgat caaggggccc ccgctgttgc cggcgttgat cgctgcatcg
     2701 gtctggatca aagtcaacgg agtgttctta ttcatccctt ttcttacact gctcacgatt
     2761 cctttggaca tggaatggga cagccccagg ggatttccca cgatgtaaac ttcttttccg
     2821 atggaaacat cggtcagttt tcccagggga agataggggt atcctccgct ccctatgtga
     2881 attagggcta aatctttatc cgggtccgtg ctgatcaccc aggcattcca ttcctggccg
     2941 tcaaaggtta cggcctgaat gtcgcccccc gcttcggcaa tcacatggta attggttacg
     3001 gcatgtccca gggggttgat taggaaggcc gagccccgcg tgctattggt tttcactcca
     3061 acgaccgccc ggctgaccag ttccaatcta ttgctggcca tttcgatgag cagattgttt
     3121 ccataatagg ggaattgggt gctgcggaat ctttcattct ggacccgggt aaatttgtcg
     3181 tcgggcggag tgtcggtgaa atggacgacc ccattcttat ctttataccg ataaacctgc
     3241 gggccggccg gggcattcaa gaacagcagt aaaaaggcca agaacatggc taaagatatg
     3301 atttttctgc ccatttcaac tcccccagac attcattctt ccattccagg aaaggctcgg
     3361 agtctccggg atcgatttcc cagtgctcat tccacggtta acaagcacgg aattacaggt
     3421 gccggtaaat attttatcgg aagaaaatga atccaactca aggagtaatt ggagtttgag
     3481 gaaggggaag gtccgtttct taagcctgcg accggcaacc ttaaacttga gacctgaggc
     3541 ctgaaacctg aaactgtttt aggtgaccac tttcgccagc tgccccgcgt aaacaatcac
     3601 cactcgcaga ataaatcctc ccagaaggac caatgaggct gtggctccgg agagccatgg
     3661 tctatgctcc ctcaggctga cccgtttgat gaaatgaggg atcagttcgt aggtttccag
     3721 ggccatggg
//
LOCUS       DASPRR010000308        11152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_556253, whole genome shotgun sequence.
ACCESSION   DASPRR010000308 DASPRR010000000
VERSION     DASPRR010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556253"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1159
                     /locus_tag="VEL68_11620"
     CDS             275..1159
                     /locus_tag="VEL68_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36658.1"
                     /translation="METILQLTINGLLLGGIYSLIAIGLTLIFGVVRIINFAQGEFLM
                     IGMFATFWIFSLLKIDPYLALFIFTPLLFLLGMGTERVIIKPIQNAPPIAQIFSTFGL
                     SIVLQNGALMLWKSDYRSLETSYSDAVISWAGLMINVPRLVAFSVAVGIVIFLYLFLQ
                     RTYLGKAMSAVAQDRDAAQLMGVDISKVYMLAFGLGIGLEGLAGALIMPIYATYPTVG
                     FTFGLIAFVVVVLGGLGNMIGAFLGGLVIGLVESFSGFYISPELNTAIYFLIFVLVLI
                     IRPQGLMGAVGAQEMGLK"
     gene            1156..2949
                     /locus_tag="VEL68_11625"
     CDS             1156..2949
                     /locus_tag="VEL68_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXZ36659.1"
                     /translation="MKNGNGSNLRWVILLAIAAILLVYPLIVRNPFYVHLFIMVFFYA
                     FLAVGWNIVGGFAGQLSLGHTVFYGIGAYSSTLLFIHLGVSPWLGMFAGGILASLTSV
                     VIGVPCFRLHGPFFAMSTLALGEVFRILATYWRDLTQGTMGISIPVKFGWKNFIFEEK
                     AVFYYVILAFTVVALGISHWIHRSRFGSNLVALREDEEAAESLGINVPRNKLWAMMIS
                     AFLVALGGTFSAQYILFIDPDAEFSVGLSLMIAIFPILGGIGTVWGPVIGAVVLVPLQ
                     EFLRAWLGSSLHGLHLFIYGGLLIIAVTFLPRGILDWVQALGRKALPFLFPTPALEKP
                     AHPRPDHFLAWSWLGQKGSSDGPILEVSDLGKNFGGLAAIRQVSFEVRRGEIVGLIGP
                     NGAGKTTLFNVISGFYPPDGGAVLYHGEAISSLHSAYRICRRGMGRTFQLVKPFQNIS
                     VLENVMTGAFLRGKTQAAARDDSLEILELVGLYELKSTLGKSLTIADRKRLELARALA
                     TRPDLLLLDEIMAGLTPKETDEAIQLIRKVRDRGITVLVIEHVMQAVMQLSDRIVILH
                     HGEKIGEGPPREVAADEKVIKAYLGEEYVIT"
     gene            2936..3646
                     /locus_tag="VEL68_11630"
     CDS             2936..3646
                     /locus_tag="VEL68_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36660.1"
                     /translation="MSLLDAEKIDVYYGDVQALREVSLKAEEGKIASIVGGNGAGKTT
                     TIRTLSGLLKPSSGAIRFLAQDVTLFPPHRRVELGMIQIPEARRLFPYMTVSGNLDMG
                     SYTARARKEKEKVRKEIFDLFPVLQERKNQMAHTLSGGEQQMLAIGRGLMARPTLLML
                     DEPSLGLAPIVVRMIFQTVLRINAQGITILLVEQNVKQCLEISNQSYVLENGRVVLHG
                     TGGEVLKNEHLRKAYLGL"
     gene            3731..5140
                     /locus_tag="VEL68_11635"
     CDS             3731..5140
                     /locus_tag="VEL68_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ36661.1"
                     /translation="MESWDAIVIGAGISGMCAASLLAGAGKRVLVLEKQDQIGGRATT
                     LSVKIAGEEYRMDNGSFHAITMADRGALGVTYERGPGLDKLKLGDLQAGMALFREDGW
                     WEMKDLVKGENRDDFKRVVESIASMSYEQAELLDTVSFDSWIRQTTRRQNVYDFFRGV
                     IWTLTTIPYPEEISAGEVLVTMKMSLDSMHRISSGTFGVGGSINLVKPLAEFITSREG
                     KVCTGMRVSRILIRKGEVRGVAVDQPLLGVGYQYPETEEIESPSVIFSVPVWDLFDFV
                     PPTEFPFWFVNTVRSYKSPLPFSTCTMGINFFMAEPVMKDTHHRVAFSLPHSQLSHQC
                     SVVSASDPTVAPPGREWISVGGGYLNEEERADRSKINAAFEALEEDLKVMYPQVFKGR
                     VLARARRLAWVIDGLKRSPYYTGRHRLPHKAPGVHGLFFAGDTVQTRGCGIDAAARSG
                     VLCAGAVLGEDIPTFRRSK"
     gene            5137..6696
                     /locus_tag="VEL68_11640"
     CDS             5137..6696
                     /locus_tag="VEL68_11640"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXZ36662.1"
                     /translation="MNLADILEETTSQNPEKICAVQDEWGPRLTYRQVLDRTWRLVHL
                     LRGLSVRKGDRVAVFLTNSFHFLEIYFSLARIGAIVVGMNFRLKGEEAAYILNHCQAK
                     VLILEERYLPLFAPLRPSVPHLLHYLVLGAPAEKMLNYEEMLNQAPPARPGREEMDEQ
                     ETIGIMYTSGTTGLPKGVEFCHKAIAVTFDYPSAIRPGIILVNVPFYHIAGALNIYTA
                     VYKNSTLVIIPQFDAAKALRLMEKEGVTETYLVPTMLRALLDHPDFLARDLSSLKRIR
                     YGAAPMPPDLILRAIRLLPCDYFNAFGQTETSGTAIALNEEDHGLVEKEEEMAKKMKR
                     LSGIGRPIPEIECRLADEQGKDVPPGIVGEILVRGPKLMKGYWNAPQETARVLKDGWL
                     HTGDMAWRDEDGYLYLADRKKDVINRGGEKIFPAEVESIINRHPKVLESAVIGVSDPY
                     WGEKVEAFVVLQPGTSASEEEMIDFCRSRLASYKKPALIKFLPELPKNAVGKVLKYVL
                     RQQASQGGPSK"
     gene            6693..8309
                     /locus_tag="VEL68_11645"
     CDS             6693..8309
                     /locus_tag="VEL68_11645"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXZ36663.1"
                     /translation="MKYSFEYYAPNRKRYKVEGVGEEPFGGVRLDVYLNRPRTLPEIF
                     TRTVQRDPRHEALVYEAERISYGELARRVDTLAYQWKREYGVRPGDRVMMLLRNTPEF
                     VVSFFAASQMGAVSVPTNTRLKAPEIEYILKDSDPAILVVEVELWERVLQVKDQAPNL
                     KAVFITGKDRVAGAILFSDLISRTAPQKTQTLATEEDVNSILYTSGTTGNPKGAMLCH
                     RNFITNSMNTQALCHLSSQDKLLVMAPLFHVTALNSQLIKIVHAGATGILMRAFKTEE
                     ILDTIEREQITEMTGVPAMYSMILQSPTLERRDLTSLRYCGYGGAPAPVEVIKTLKEK
                     FPTWRLRNVYGLTEASSWVTMLPHEQTTIRPDSTGLPVPVVRLKIANDRGQEVSPGEV
                     GEILIKGPNIFKGYWRKPEATAESLRDGWFYTGDLGRFDEEGYLYVVDRKKEMVIRGG
                     ENIYCIEVENVLYQHPKVAEAAVIGVPDPILGEVVKAVCVLKKGQKASELEIQEFCKK
                     YLADYKVPKNVCFIDELPRNPAGKVIKKAL"
     gene            8326..9789
                     /locus_tag="VEL68_11650"
     CDS             8326..9789
                     /locus_tag="VEL68_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ36664.1"
                     /translation="MVTSEEYRKRLYAMRPNIRLFGRQVGRDDPNIQPGIRTISLTYD
                     LARDPQYQSLICTSSSLTGRKISRFNHLSQTAQDLIDKIRMTRACCQKLTYCIQRCMG
                     IDGLQALGEVTFQMDRKKGTAYHPRFLEYMKFFQEEDLVIAGAMTDVKGDRNLRPHQQ
                     ADPDLYLHVVERRDEGIVVRGAKAHTTAGPYAEELLVLPTRAMTEKDADYAVSFAIPA
                     DTSGIHLIAKVKAWPKREQMKNAITGYGASESITVFDNVLVPWDRVFMCGEWEYAGVL
                     ASMFSRFHRHSYCGCKPAIVDSQIGASALLARYNGVEKSSHVRQKLVELITTAEQVYA
                     CGIASAIMGKKSDSGIFLPNSIYCNSGRYLAGQKFHQGFEIVHDIAGGLISTLPDERE
                     FLDEFSGPYLQKYLKGKEGFSAEDRVRAYRLIEDITVSQRAAQNLVGGLHGGGSPEME
                     RIAILADYDLEEKIRMAQELAGIGQVMKEGKGSGGQG"
     gene            9818..10780
                     /locus_tag="VEL68_11655"
     CDS             9818..10780
                     /locus_tag="VEL68_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXZ36665.1"
                     /translation="MEISVKDLLAMHYWMVLDRVLEEKTLELLKLGKLVGFHHPNIGQ
                     EGVDVGTCFGLRKDDTIMPTHRGKGKYLMKGVPLETMMAGMFGRRNGCGKGRGPASHS
                     GDNSVGLLAGTGLIGSGIPISTGAALAMKLQKKDSVALHFFGDGASNRGDFHESLNIA
                     GVMKLPVVYVCDNNCYAISVPQTCAMAITDIAIRASSYGFPGLVVDGNDVLAVYEATQ
                     QAIARARRGEGPTLIECKTYRWMGHSMNDPGVYRTPDEVEVWKQKCPIQRFEAHLRQK
                     NLLDDQTTREVYRRVNEEIGDAVRFAEASPLPAPEDTLLDVYAG"
     gene            10919..>11152
                     /locus_tag="VEL68_11660"
     CDS             10919..>11152
                     /locus_tag="VEL68_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012369824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXZ36666.1"
                     /translation="MREMTFVEAINEAMHEEMARDEKVYVFGEDVRVGYGRGGVFGAT
                     NGLLAKFGPDRVIDTPISESAIAGISVGAALWGM"
BASE COUNT         2450 a         3063 c         3196 g         2443 t
ORIGIN      
        1 gccgggcaga acatgcgcgc cctctggttc gtagaacaat ggagggatag aaagctgcat
       61 tgcgcctggc ctgacgatgc caaagaggcg gggtaccagg tgattttgcc ctaccccacc
      121 tgggaagagc gaaagaaaaa gtagactgag aattcttggg atggcgtggg ggcaattcat
      181 gaattgcccc cacgctggca tcgtcaaccg cttggttagg gaaggtttgt ttttctcgat
      241 atctttctat ccagcctttt aggagcatcc gatcttggaa accattcttc aactgaccat
      301 taacggcctc ctgctgggag gaatctattc cctaatcgcc atcggcctta ccctgatctt
      361 tggggtggtg cggatcatca acttcgccca aggggagttc ctgatgatcg gaatgtttgc
      421 caccttctgg atcttcagcc tgctcaagat cgacccctac ctggcgcttt ttatcttcac
      481 tcccctcctc tttcttctgg gcatggggac cgaacgcgtg atcatcaagc ccatccagaa
      541 tgccccaccc attgcccaga ttttctccac cttcgggctc tccatcgtcc ttcagaacgg
      601 agccctgatg ctctggaagt ccgactaccg gagtttggaa acctcctact ccgacgcagt
      661 gatcagctgg gcaggtttga tgattaatgt accccgtctg gtggctttct cggtagctgt
      721 ggggatcgtg atctttcttt acctattttt gcagagaact tatttgggca aggccatgag
      781 cgcggtcgcc caggaccggg atgccgccca attgatgggg gtggatatca gcaaggtcta
      841 catgctggcc ttcggcttgg gaatcggcct ggaagggctg gctggagctc tcatcatgcc
      901 catttatgcc acctacccca cggtgggatt caccttcgga ttgatcgctt ttgtggtcgt
      961 cgtcctgggc ggcctgggca acatgatcgg agcctttctg ggcggcctgg tcatcggatt
     1021 ggtggaatcc ttcagcgggt tctacatctc tcccgaactg aacaccgcca tttactttct
     1081 gatattcgtt ctggtcctca tcatcagacc ccaggggctc atgggggccg tgggtgccca
     1141 ggagatgggg ttaaaatgaa gaacggaaac ggctcgaact tacgctgggt tatcctgttg
     1201 gccatagcgg ccatcctcct ggtctatcct ttgatcgtcc gcaatccctt ctatgttcac
     1261 cttttcatca tggtcttctt ttacgccttt ctggccgtag gctggaatat cgtgggaggg
     1321 tttgcaggcc agctttcttt agggcacacg gttttctacg gcatcggagc ctactcgtcc
     1381 actttgcttt tcatccattt gggggtctct ccctggttgg gcatgttcgc cgggggaatt
     1441 cttgcctccc tgacctccgt ggtcatcgga gtcccctgct ttcgtcttca cggacccttt
     1501 tttgccatga gcaccctagc cttgggcgag gtttttcgaa tcttggccac ctactggagg
     1561 gatttgaccc aaggaacgat gggaatctcc atcccggtca agttcggatg gaaaaatttc
     1621 atcttcgaag agaaggcggt cttttattat gtaatcctgg cttttacggt cgttgccctc
     1681 ggaatctccc actggatcca ccgttctcga tttggctcca acctcgtggc cttgcgggaa
     1741 gatgaagagg cggccgaatc attggggatt aacgtccccc ggaacaaact ctgggccatg
     1801 atgatcagcg cctttctcgt cgccctcggc gggaccttca gcgcccagta catcctcttc
     1861 atcgacccgg atgcggagtt cagcgtcggc ctctccctca tgatcgccat atttcccatc
     1921 ctggggggaa tcgggacggt ttggggtccg gtgattggcg cggttgtcct cgtccccctg
     1981 caggaatttt tgcgcgcctg gctggggagc tctctccatg gtctgcatct cttcatctat
     2041 ggggggcttt tgattatcgc cgtcaccttc ctcccccggg gaattttgga ctgggtccag
     2101 gccttgggaa gaaaggccct tcctttcctc ttccccactc cggccctgga aaagccggcg
     2161 cacccgaggc cggaccattt cctggcctgg agctggctgg gtcaaaaagg aagttcggat
     2221 gggccgatcc tggaagtaag tgacctgggc aagaacttcg gtggtctggc ggccattcgt
     2281 caggtttcct ttgaggtcag gaggggagag atcgtcggtc tgatcggccc taacggggcg
     2341 gggaagacga cgctcttcaa cgtaatctcc gggttctatc cccccgacgg aggagccgtt
     2401 ctctaccacg gagaggcgat atcttctctc cactccgcct atcgaatatg ccgcaggggc
     2461 atgggacgga cctttcagtt ggttaagccc tttcagaaca tttccgtcct ggaaaatgtt
     2521 atgaccgggg ccttcctgcg aggcaagacc caagccgcag cccgggatga ctccctggaa
     2581 atcctggaac tcgtaggcct ttacgaattg aagagcacct tggggaagag tctcaccatt
     2641 gccgaccgga aacggctgga gctggcccgg gctctggcca cccggcccga ccttcttctc
     2701 ctggatgaga tcatggcggg gctgaccccc aaagagacag acgaagcaat ccagttgatc
     2761 cgcaaggtcc gagaccgtgg gattaccgtc ctcgtgatcg agcatgtcat gcaggcggtc
     2821 atgcagcttt cggatcggat cgtcattctg caccacgggg aaaagatcgg cgagggtccc
     2881 ccccgggaag tcgccgccga tgaaaaggtg attaaagcct atttgggtga ggaatatgtc
     2941 attacttgat gcggaaaaga tcgatgtata ttatggagat gtccaggctt tgcgagaagt
     3001 ctccctgaag gcggaagagg gaaaaatcgc tagcatcgta ggagggaatg gggccggaaa
     3061 gacgacgacc ataagaaccc tttctgggct cctgaagccc tcctcggggg ccatccgatt
     3121 tcttgcgcag gacgtgaccc ttttcccccc tcaccggaga gtagagctgg gtatgatcca
     3181 gattcccgag gcccgccgtt tgtttccgta tatgaccgtc tccgggaacc tggatatggg
     3241 ctcgtacacg gcccgggccc gcaaagaaaa ggaaaaagtc agaaaagaga ttttcgatct
     3301 cttccccgtt ctccaggagc gaaagaacca gatggcccac acccttagcg gaggggaaca
     3361 gcagatgctg gccatcggcc ggggtctcat ggcccggccc acattgctga tgctggatga
     3421 accatcgctg ggcctggctc ccattgtcgt caggatgatc tttcaaaccg tgctccggat
     3481 caacgctcaa ggaatcacca tactcctggt ggaacagaat gtgaaacagt gtctggagat
     3541 atctaaccaa agctatgtcc ttgaaaatgg gagagtcgtg ctccatggaa ccggagggga
     3601 ggttctgaag aacgagcacc tccggaaggc ttatttgggg ctttaaacga ggggcaggtc
     3661 gcgggtttca cctttcccgg gaaaaagaaa taatccctaa ggcgatctcc tctcaaggaa
     3721 ggaaactcgg atggaaagct gggacgcaat cgttatcggg gccgggatat cgggcatgtg
     3781 tgcggcttct cttctggctg gagccggaaa acgggtactg gttttggaga agcaggacca
     3841 gatcgggggg cgggcaacga ccctcagcgt aaagatcgcc ggcgaagaat accggatgga
     3901 caacggcagc ttccacgcca taaccatggc cgaccgggga gccttgggtg tgacttacga
     3961 gaggggcccg gggttggaca agctgaaact cggtgacctg caggcaggga tggctctctt
     4021 ccgggaagac ggctggtggg aaatgaaaga cctggtcaag ggagagaacc gggatgactt
     4081 caagcgggtg gtggaatcga tcgcttccat gagttacgaa caggcggaac ttctcgacac
     4141 cgtctccttc gattcctgga ttcgccagac cacccgccgg cagaatgtct atgacttctt
     4201 ccgcggcgtg atctggaccc ttaccaccat tccctatcca gaagagattt ccgccgggga
     4261 agtcctggtc acgatgaaaa tgagcctgga ttccatgcac cgcatctctt ccgggacttt
     4321 tggggtcggg gggtccatca acctggtcaa acctctggcg gaatttatca cctctcggga
     4381 aggaaaggta tgcaccggca tgcgggtctc cagaatccta attcgaaaag gcgaagtcag
     4441 gggcgttgcc gtcgaccagc ctttgctcgg agtcggatac caatacccgg aaaccgaaga
     4501 gatcgaatcc ccatcggtca tcttcagtgt tcccgtatgg gacctgtttg acttcgtccc
     4561 ccccaccgaa ttcccctttt ggtttgtcaa taccgttcgg agttacaaat cccccctccc
     4621 cttttccacc tgcaccatgg gcatcaactt tttcatggcc gagccggtca tgaaggacac
     4681 tcaccaccgg gttgcttttt ctcttcccca ctcccagctc tcccatcagt gctcggtggt
     4741 ctccgcctcc gaccccaccg tagctccccc ggggcgggag tggatcagcg tgggaggagg
     4801 atacttgaac gaggaggagc gagcggaccg ttccaagatc aacgccgctt tcgaggccct
     4861 ggaagaagac ctgaaagtca tgtatcccca ggtcttcaag gggcgggtcc tggcccgggc
     4921 ccgtcgtctg gcctgggtga tcgacgggct gaagcgcagt ccttattaca ctgggcgcca
     4981 tcgtctcccc cataaagctc ccggagttca cggtctattt tttgccgggg atacggtcca
     5041 aacccggggc tgtggaatcg atgccgcggc ccgctccggg gttctatgcg ctggtgcggt
     5101 gcttggagaa gatatcccca ccttccggag atcaaagtga atcttgccga tattctggag
     5161 gaaacaacct cccaaaatcc ggaaaagatt tgcgccgtcc aggacgaatg ggggccaagg
     5221 ctcacctacc ggcaggttct cgaccgaacc tggcgactgg ttcatctcct gcggggctta
     5281 tctgtccgta aaggggaccg ggtggctgtc tttctgacta attccttcca ctttctggaa
     5341 atctactttt ctttagcccg gatcggggcc attgtcgtgg ggatgaattt tcgcctgaag
     5401 ggagaagaag ccgcctatat tctgaatcat tgtcaggcca aggtcttgat tctggaagag
     5461 cgctatcttc ctctctttgc tccccttagg ccttccgttc cccatctcct ccactatctg
     5521 gtcctgggag cccccgcgga gaaaatgttg aattatgaag agatgctcaa ccaggccccc
     5581 cctgcccgcc cagggcggga agaaatggac gaacaagaaa ccatcggaat catgtacacc
     5641 agcgggacta cgggtctgcc caagggagtc gaattttgcc acaaagcaat cgccgtcact
     5701 ttcgactacc ccagtgccat ccggccgggg atcattctgg tcaacgttcc tttttaccac
     5761 attgccggcg ccctgaatat ttacactgcg gtatataaga attccaccct ggtgatcatc
     5821 cctcagttcg acgcagccaa agcattgcgc ctgatggaaa aggagggagt aaccgaaacc
     5881 tacctggtcc caaccatgct tcgggctctt ctggatcatc cggatttttt ggcgagggac
     5941 ctttcctcct tgaaacgcat ccgctatgga gccgcgccca tgccccctga ccttattctg
     6001 cgggcaatcc ggctcctccc ctgcgactat ttcaacgctt tcggacagac cgagacctcc
     6061 ggaactgcca ttgctctcaa cgaggaagac catggcctcg tggaaaagga ggaggaaatg
     6121 gccaagaaga tgaagagact ttcgggcatc ggacgcccca ttccggagat cgaatgccgc
     6181 ctggcggatg aacaagggaa agacgttccc ccgggaatcg tcggagagat cctggttcgc
     6241 ggacccaagc tgatgaaggg gtactggaac gctccacagg aaacagcccg ggttttgaag
     6301 gacggatggc tccacacggg cgatatggcc tggagggacg aagacgggta cttatacttg
     6361 gcggaccgga agaaggacgt catcaaccgg ggaggagaga aaatctttcc cgcggaagtg
     6421 gagtcgatca tcaaccgcca tcccaaggtt ctagaatcgg cggtgatcgg agtttccgat
     6481 ccttactggg gagaaaaggt ggaggctttc gtcgttctcc agccggggac ttcggcgtcg
     6541 gaggaagaga tgattgattt ttgccgttcc cgactggcaa gttacaaaaa gcccgcattg
     6601 attaagtttt tgcctgaatt gcccaaaaac gccgtgggaa aagtcttgaa gtatgtcctt
     6661 cgccaacagg cctctcaagg aggaccgagc aagtgaagta ctcattcgaa tactacgctc
     6721 ccaaccgaaa aagatacaaa gtcgaaggcg taggagaaga acccttcggc ggggtccgcc
     6781 tggatgttta cctgaaccgc ccccgaactc ttccggagat ttttacccgg actgttcaga
     6841 gggacccgcg gcatgaggcc ttggtgtatg aggcggagag gatttcctac ggcgaactgg
     6901 cccggagggt ggacaccctg gcctatcagt ggaagaggga gtacggcgtc cgcccgggag
     6961 accgggtgat gatgctctta cgaaacactc cggaattcgt ggtttccttt tttgccgcgt
     7021 cccaaatggg cgccgtgtcc gttcccacga atacccgcct caaggccccg gagattgagt
     7081 atattctgaa ggattcggac ccggccatcc tagtcgtcga ggttgaactg tgggagaggg
     7141 ttcttcaggt caaagaccaa gcccccaacc taaaagccgt cttcattacc ggaaaagacc
     7201 gagtcgccgg agccattctt ttctccgatc tgatcagtcg caccgcaccg cagaagaccc
     7261 agaccctggc cacggaggaa gacgtgaaca gcatcctcta tacttcggga actaccggga
     7321 atcccaaagg ggccatgctc tgccaccgca actttatcac caactccatg aatacccagg
     7381 ctttatgcca cctctcctct caggataagc tcctggtcat ggctccccta ttccatgtga
     7441 ccgccctcaa ttcacaattg atcaagatcg tccatgccgg agccactggg attttgatgc
     7501 gggctttcaa aaccgaagag atcctggata cgatcgagcg ggaacaaatc accgagatga
     7561 ccggagtccc ggccatgtac tccatgattc tccagtctcc gactctcgaa cgacgagact
     7621 taacatcttt gcgttattgc gggtacgggg gcgccccggc tccggtggag gtgatcaaga
     7681 ccctgaaaga aaaattcccc acctggagac tgcgcaatgt gtatggcctg accgaggctt
     7741 cctcgtgggt gaccatgctt ccccatgaac agaccaccat tcgcccggac tccaccggtc
     7801 tccctgttcc ggtggtgcgg ctgaagattg ccaacgaccg cgggcaagag gtctccccgg
     7861 gcgaggtcgg tgaaattctc atcaaaggac ccaatatatt taaaggatac tggaggaaac
     7921 cggaggccac cgccgagtct ctcagagacg ggtggtttta caccggggat ttgggcaggt
     7981 tcgacgagga aggatatctc tatgtagtgg accggaagaa ggaaatggtc attcggggcg
     8041 gggagaacat ctactgcatc gaagtggaaa atgtacttta ccagcatcct aaggttgcgg
     8101 aggcggccgt aatcggagtt cctgacccga tcctaggaga ggtcgtgaag gcggtttgtg
     8161 tcttgaagaa gggccagaag gcttcagaat tggaaatcca ggaattctgc aaaaaatacc
     8221 tggcggatta caaagttccc aaaaatgtgt gcttcatcga tgagctcccg cgcaatcccg
     8281 cgggaaaggt gatcaaaaaa gctttataac ccaggaggat ccccgatggt gacttccgag
     8341 gagtaccgca aacgactgta tgccatgagg ccgaacatcc gcctcttcgg gcgacaggtc
     8401 ggccgggatg accccaacat ccagcctggc atccggacca tctcactgac ctatgaccta
     8461 gcccgggacc cccaatatca aagcctaatt tgcacctctt ccagcttaac gggcagaaag
     8521 atcagccgtt ttaatcacct gagtcagacc gcccaggact tgatcgacaa gattcggatg
     8581 acccgggcat gttgccagaa gcttacttac tgcatccagc gttgcatggg aatcgacggg
     8641 ttacaggctc tgggcgaggt caccttccag atggaccgta aaaaagggac tgcctaccat
     8701 ccccggttcc tggagtacat gaaatttttc caggaagaag acctggtcat cgccggggcc
     8761 atgaccgacg tgaagggcga taggaacctg cgcccgcacc agcaggcgga ccctgacctc
     8821 taccttcacg tggtggaaag gcgggatgaa ggaatcgtgg ttcggggagc caaagctcac
     8881 accaccgcag gcccctatgc ggaggagctg ctggttttac ccacccgggc tatgaccgaa
     8941 aaagatgccg actatgcggt cagcttcgcc atcccggcgg acacctcggg cattcacctg
     9001 atcgccaagg tgaaggcctg gccaaagcgg gagcaaatga agaacgccat cactggatac
     9061 ggagcctctg agtccattac cgtttttgac aacgtcttgg tcccgtggga ccgggtgttc
     9121 atgtgcgggg aatgggagta tgccggggtt ctggctagca tgttctcccg ctttcaccgc
     9181 cactcctact gcggatgcaa gcccgccatt gtggacagtc agatcggggc ttccgctctc
     9241 ttggctcggt acaacggggt agagaaatct tcccatgtac ggcagaagct ggtcgagctc
     9301 atcaccaccg cggagcaggt ctatgcctgc ggcattgctt cggcgatcat ggggaaaaaa
     9361 tctgattcgg ggatcttctt gcccaatagc atctactgta actctggacg gtatttggcc
     9421 ggccagaagt ttcaccaggg cttcgaaatc gtccatgata ttgccggcgg tctgatctcc
     9481 acccttcccg acgagcgaga atttctggat gagttctcag ggccctacct ccagaagtac
     9541 ctgaagggga aggaaggatt ctctgccgag gaccgggttc gcgcctaccg tctgatcgaa
     9601 gacatcaccg tctcccagcg ggcggctcaa aacctggtgg gcggcttgca cggaggcggc
     9661 tccccggaga tggagaggat tgccattctg gccgactatg acctggaaga gaagattcgc
     9721 atggcccaag aactggccgg gatcggtcag gtcatgaagg aagggaaagg aagcgggggt
     9781 caaggttgat tcctgttaat gtaaggtagg aggttctatg gaaatttcgg tgaaagattt
     9841 gctggccatg cactattgga tggtcttgga ccgggttctg gaagaaaaga cgctggaact
     9901 cctgaaattg gggaaactgg tcgggttcca tcaccccaac atcgggcaag aaggagtgga
     9961 tgtgggaacc tgctttgggc tgcggaagga tgacaccatc atgcccaccc atcggggcaa
    10021 aggcaagtac ctgatgaaag gcgtaccgct cgagaccatg atggccggga tgttcgggag
    10081 gaggaacggc tgtgggaagg ggcgtgggcc ggcctctcac tcgggggaca attccgtcgg
    10141 ccttctcgcc ggcacgggcc tcatcggttc ggggatcccc atcagtaccg gtgcagccct
    10201 ggccatgaag cttcagaaga aggacagcgt ggccctgcat ttcttcgggg acggggcttc
    10261 taaccgagga gatttccatg agtccttaaa catagctgga gtcatgaagc ttccggtggt
    10321 ctacgtctgc gacaataact gttatgccat ctccgtcccc caaacctgcg ccatggccat
    10381 caccgatatc gccatccggg cgtcgagcta cgggttcccc ggcttggtgg tcgacggcaa
    10441 tgacgtcctc gccgtgtacg aagcgaccca gcaggccatc gccagggccc gcagaggaga
    10501 aggaccaacc ctgatcgaat gcaaaacgta tcgctggatg ggacacagca tgaacgaccc
    10561 gggagtctac cgtacccctg atgaggtgga ggtgtggaag caaaaatgcc caatccagcg
    10621 tttcgaagct cacttgaggc aaaaaaatct gctcgatgac caaaccacca gagaggttta
    10681 ccggcgggtg aatgaagaga tcggggatgc ggtccggttt gccgaagcga gccccttgcc
    10741 tgcacccgag gacacgcttc ttgacgtgta tgctggttaa gaaaaatggg acgcagatga
    10801 atgcagatca acgcagagaa atcccacgat gttaaaggca aaggcatttg gggacctttt
    10861 gaatcctgta aatctgcgtt catctgtgtc caataatggt ttttgaggag aaataagaat
    10921 gagagaaatg accttcgtcg aagccatcaa cgaagccatg catgaagaaa tggcccggga
    10981 tgaaaaggtg tatgtatttg gagaggacgt ccgggtcggg tacggccggg gcggcgtgtt
    11041 cggggccacc aacggccttc ttgccaaatt cgggcccgat cgggtgatcg atacgcccat
    11101 ttcggaatcg gccatcgccg ggatatcggt gggggccgcc ctctggggaa tg
//
LOCUS       DASPRR010000309         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_877835, whole genome shotgun sequence.
ACCESSION   DASPRR010000309 DASPRR010000000
VERSION     DASPRR010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877835"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..2524)
                     /gene="nuoG"
                     /locus_tag="VEL68_11665"
     CDS             complement(26..2524)
                     /gene="nuoG"
                     /locus_tag="VEL68_11665"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HXZ36667.1"
                     /translation="MPKLKIDGLEIEVPKGMKVIEAAERLGIVIPRFCWHPALGPAGA
                     CRVCAVMFLEGPFKGIQMSCMVDAQDGMAVSTVHPEAAAFRKQVAEWLMMNHPHDCPV
                     CDEGGQCLLQDMTVSGGHGIRRYRGKKRTYNDQDLGPFIQHEMNRCIHCYRCVRFYQA
                     FAGYRDLGVMQSAYRTYFGRYQDGPLESPFSGNLTDICPTGVYTDKTARFKGRRWDFE
                     RGPSLCIHCSLGCRTVGSARYREMVLMEAEFNGAVNGYFICDRGRFGFHYVNHPERRR
                     RARLKKEEVSPQEALDISVRTLKKILGESGPSSIAALASSRTSLENQGMLLRLCRRMG
                     WRAPGFFATRDAALKVSRAASTLDSRNAVSLRQVEEADFILVLAADPVNEAPMLALAL
                     RQASLRKGRVVVLDPRPVFLPLDFDHLPLSPAEMETFFGALVKRTADPSSATLLGPKA
                     AQFLDSLPGEYPEGRFKNRLLSLEADLKESQSPVLLCGMDLLPPSFLDLVGAGARILR
                     GAKGMPGLFYLMPGANSLGAVLLAPEPGSVEQVVEEIEKGKIRALLIVESDPFWLFPD
                     RQRLEKALAKLDFLLAMDYLPSKTWDSAHVFFPTATLFETESSFVNQEGRLQSARPFY
                     GGGSPVSQVGAGSHPPRTFGAEVPGGESRPAWRILAELEKCLSGQGTEPFEPTPQGLW
                     EWMAGEIPLLAGVKPFDSPEGVRLLGERKGGDSFPSRVGAPASSPAEECLNLFCVEWT
                     FGTEELSCYSRFAQQAEKSPCLFLYTGDAARLGLGDKDRVRIELERGSLEVEVSTAEN
                     MAAGVMILPRHRRLSWQVMPVLPARVTPDKIKKI"
BASE COUNT          575 a          839 c          798 g          527 t
ORIGIN      
        1 aaaatccgtg gccagcaaca agtgactaga ttttcttaat cttgtccggc gtcactctgg
       61 ctggcaacac gggcatcacc tgccaggaaa gccgccggtg ccgcggcaat atcatcactc
      121 ctgcagccat attctccgca gtggagactt ccacttccaa agagcccctc tccagttcga
      181 tccgaacccg gtctttgtca cccaacccca gccgggccgc gtccccggta tagagaaaca
      241 ggcaggggct tttttccgcc tgttgagcaa agcgggagta gcaggaaagc tcctccgttc
      301 caaaggtcca ttcgacacag aagaggttca gacattcctc cgcaggagag gatgcgggcg
      361 cgccaacccg ggaagggaaa gaatccccgc cctttctttc cccaaggaga cggactccct
      421 ccggagagtc gaagggtttg accccggcca aaaggggaat ttccccggcc atccattccc
      481 atagaccttg gggagttggt tcgaatggct cggttccttg gccggacagg cacttttcca
      541 gctcggccaa aatcctccat gcagggcggg attcccctcc cgggacctcc gctccgaagg
      601 ttcggggagg atggctgcca gcgccgacct ggctgaccgg gcttcccccg ccgtagaagg
      661 gccgggcgga ctggagccgc ccctcctgat tgacaaagct cgattcggtc tcgaagaggg
      721 tcgccgtggg aaaaaagaca tgcgccgaat cccaagtctt cgagggcagg taatccatgg
      781 ccagaagaaa atcgagtttg gccagggctt tctctaacct ctgccgatcc ggaaagagcc
      841 aaaagggatc gctctccacg atgagcaggg cccgaatctt gcctttttca atctcttcca
      901 cgacctgctc gaccgaacca ggttccggcg caagcagcac tgcccccagt gaatttgccc
      961 ccggcatcag gtagaaaagc cccggcattc ctttggcccc tctcaggatc cttgctccgg
     1021 cacccaccaa atccagaaaa gacgggggaa ggaggtccat cccgcataag agaaccgggc
     1081 tttggctctc tttcaggtca gcttctaagc tcaagagacg gttcttgaac ctcccttccg
     1141 ggtattctcc aggaagcgag tcaaggaatt gagccgcttt cggccctaag agggtcgctg
     1201 atgacgggtc tgcggtcctc ttgactaggg cgccgaagaa ggtctccatt tccgctggtg
     1261 aaagagggag atggtcaaaa tcaaggggaa gaaagaccgg ccgcgggtcc aggaccacaa
     1321 cccggccttt cctgagggat gcctgccgca gggccagggc gagcatgggg gcttcattca
     1381 ccgggtctgc tgcgagaacg aggatgaaat cggcttcttc gacctgccgg agggacaccg
     1441 cgttccggga atccagggtg gaggcggccc tggacacctt taaggcggcg tcccgggtgg
     1501 caaagaagcc gggggccctc cagcccatcc ggcggcagag acggagcagc atgccctggt
     1561 tctccaggct cgtgcgtgaa gaggccaggg cagcgatgga agaggggccg ctctccccga
     1621 ggatcttttt cagggtccgg acggaaatgt ccaaggcttc ctgaggggag acctcctctt
     1681 tcttcagccg cgcccgtctc cgcctctccg gatgattgac atagtggaag ccgaaacgcc
     1741 cccggtcgca gatgaagtac ccattcaccg ccccattgaa ctcggcctcc atgaggacca
     1801 tttcccggta acgagcgctg cccacggtgc ggcatcccag ggaacagtgg atgcaaaggg
     1861 aaggccctct ttcgaaatcc caccgccgcc ctttgaaccg ggcggtcttg tcggtgtaaa
     1921 cccccgtcgg acagatgtcg gtcaggttgc cggagaaggg gctctccagg gggccgtcct
     1981 ggtagcggcc aaagtaagtt cggtaggcgc tctgcataac cccgaggtcg cggtatccgg
     2041 cgaaagcctg atagaatctc acgcagcggt aacaatggat gcagcggttc atctcgtgct
     2101 ggatgaacgg tcccaggtcc tggtcgttgt aggtcctctt ctttccccgg tagcggcgga
     2161 ttccgtgccc gccggagacg gtcatgtcct ggagaaggca ctgtcctccc tcatcgcaga
     2221 ccgggcagtc gtggggatga ttcatcatga gccattcggc cacctgcttg cggaaagccg
     2281 ccgcctcggg atgaacggtg gagaccgcca tgccatcctg ggcgtccacc atgcagctca
     2341 tctggattcc cttgaacggt ccctccagga acatcaccgc acatacgcgg caggctccgg
     2401 cgggtcccag ggccgggtgc cagcagaagc ggggaatcac gattcccagg cgctcagcgg
     2461 cctcgatcac tttcatccct ttgggcactt caatttccaa accgtcgatc ttcagtttgg
     2521 gcatataaaa gaagctttca gcaatcggct ttcagcagtc agctaaaaca agaaaatgat
     2581 cggaccagcc ctcaacaaac ctctcaggga accttgggga cttctttggg ctgatcgctg
     2641 atggctgacc gctgatcgct gcctatggaa taggggcatt tcttctgact gatgtgctcc
     2701 agcacctcat cggggaaata agtcaagaga ctctccacc
//
LOCUS       DASPRR010000310         4944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_877852, whole genome shotgun sequence.
ACCESSION   DASPRR010000310 DASPRR010000000
VERSION     DASPRR010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877852"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VEL68_11670"
     CDS             complement(<1..390)
                     /locus_tag="VEL68_11670"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36668.1"
                     /translation="MKKYLIRRLLSGIVVLWLVLTLVFILIHSVPGDPAQIIAGDLAD
                     AKTVAEIRAKLGLDQPLWKQYWDLLRGAMTGDMGVSFSYRYSAFRVVIERLPATIELT
                     LVGAILAIPISIALGIVSAVKRNSALDL"
     gene            complement(476..2047)
                     /locus_tag="VEL68_11675"
     CDS             complement(476..2047)
                     /locus_tag="VEL68_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36669.1"
                     /translation="MEWKYPISRRDFLKLTGVMSATMFGTPIWGERNLAWGQDKGVFE
                     EFGTAYSETIVTLDPQNTTALGNGLLNPYIFDFPLDRDGQTNEMVPQAATGWEAKSDT
                     VWRFYMRQGIKFWNGNPLTAKSFAFTQNRILDPKFVSPQRNWFAQVDKAVAVDSYTVD
                     ILCKTPFPAYPALMSNLAILDEQYYGSHNLDHTTANPMGSGPFIFKKWNRGIGVEMEV
                     NKNYWMPPPPIKVLKYYGIQEAETRTASLLSGQTKIISGIPLDHFERIQKAGLRAEGK
                     PGPRIVFGAFNQKIKPFDDMRVRQACNFAVDNRKIMNVFMKGMGEVMNQPLGSAIFGH
                     NPKLSFYDVNLDKAKSLMGAAGYPNGFPHPITLELVPGYALNLLEICEAVAYDLRRIG
                     IQVKVQPRENADFRARRPAATGDPNFGPLFAGSWGVASFDASSYLPLLLDRNGAYGRN
                     YDEKAEKWIEKTNSIVDKQKRLKELQDLEAYFHEQCPFIWLHVQPNTYAMSTDHDFKA
                     RVDERLTVTNMKKIK"
     gene            complement(2203..3195)
                     /locus_tag="VEL68_11680"
     CDS             complement(2203..3195)
                     /locus_tag="VEL68_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36670.1"
                     /translation="MESILEVHGVKKYFFDHRGLLEIVFGGMAPPVRAVDGVSFRVRK
                     GECLGLVGESGCGKTTVARLILRIYDLTEGEIHFEGQDIHSLSRSELKAIYQRVQIIF
                     QDPRTSLDPRQKVAQIIGEPLEIHGKGSGKERRDRVMELLSLTGLSPVHADRYPHEFS
                     GGQQQRIAIARALAIDPAFVICDEPVSALDVSIQGQILNLLMDLQEKFGLSYLFITHD
                     LAVARHICNRIGVMYLGKIMEVGETEEIFDNPLHPYTRALLSAIPHPDPELALQQIIL
                     KGDPPDPRRPPQGCRFSGRCPEALPECAEMEPALKEWEGGHTAACLLLEKPMRR"
     gene            complement(3293..4297)
                     /locus_tag="VEL68_11685"
     CDS             complement(3293..4297)
                     /locus_tag="VEL68_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36671.1"
                     /translation="MNCPYFVEDEHQTHMSLLEVKNLEVIFPTLWGTVRAVNRVDFRQ
                     ETGETVGLVGESGAGKSMLGRSLMGLIPYPGQIPRGQIFWKGEDLRQKREKEVRKIRS
                     KEIAMVFQDPISALNPLKKIGDQLLEAVFLHQNLRKEEAMGLAIRRMGDVGIPRPEIS
                     IQNFPHQYSGGMLQRIVIALALINDPALVIADEPTTALDVTIQAQILQLLKDLQSARK
                     MSVLMITHNLGIVAQFCSRIYVMYAGKMVEHADTHTLFLRPLHPYTRGLLRSVIRLNQ
                     KTRRVETMAGLMPDLIHMPPGCAFHPRCDQSRDVCRQETPEWREVEKEHWVACHQKSR
                     "
     gene            complement(4601..>4944)
                     /locus_tag="VEL68_11690"
     CDS             complement(4601..>4944)
                     /locus_tag="VEL68_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ36672.1"
                     /translation="WELSKEIEAVTVQYSVKEMGEMLDRERVANSPIFNIQQVVEDPH
                     LKERGYFVEIDHPIIGRAKLSGFPFKLSKTPGQIERTSPLVGEHNELILGKYLQIGGK
                     DVAQLRREGAI"
BASE COUNT          990 a         1441 c         1361 g         1152 t
ORIGIN      
        1 aaggtccaaa gccgagtttc tcttcacggc gctcacgatt cccagggcga tggagatggg
       61 aatggccaag atggctccca ccagggtgag ttctatcgtg gccggcaggc gttcgatgac
      121 cacccggaag gccgagtacc ggtaggagaa agaaaccccc atgtctccgg tcatggctcc
      181 ccggaggagg tcccagtact gtttccagag cggctggtcc aggccgagct tggcccggat
      241 ctcggcgacc gtcttggcat ccgccaggtc tccggcgatg atctgcgccg ggtctcccgg
      301 cacggaatga atcaggatga acaccagggt gagcaccagc cagagaacca caatcccgct
      361 caagagacgt ctgattaaat atttcttcat tccctgcgtc cggcggtagg ggcggttcgc
      421 gaaccgcccc tacattttat ctcttttccc atacactcac actgtcactg cttttctatt
      481 tgatcttctt catattcgtt accgtaagtc tctcgtccac gcgcgccttg aaatcatgat
      541 cggtgctcat ggcgtaagta ttgggctgga cgtgcagcca gatgaagggg cactgttcgt
      601 ggaagtaggc ttccaggtct tggagctcct tcaggcgttt ctgcttgtcc acgatgctat
      661 tggtcttctc gatccatttt tccgccttct cgtcataatt tcgcccgtag gcaccgttgc
      721 ggtccagaag gagggggaga tagctcgaag cgtcaaaaga agctaccccc caggacccgg
      781 caaagagagg gccgaaattg ggatcgcccg tggccgccgg ccgccgggcc cggaaatcgg
      841 cgttctcccg gggctggacc ttcacctgga ttccgatcct gcggagatcg taagcgaccg
      901 cttcacagat ctccagcagg ttcaaagcgt aaccggggac aagctccagc gtgatcggat
      961 ggggaaagcc attcgggtat cctgcggccc ccatgaggct cttggctttg tccaggttga
     1021 catcataaaa agagagtttc gggttatgcc cgaaaatcgc cgaccccaag ggctggttca
     1081 tcacttctcc catgcccttc atgaagacat tcatgatctt gcggttatcg actgcgaagt
     1141 tgcaggcctg ccggacccgc atgtcgtcga agggtttgat cttctggtta aaggcgccga
     1201 aaacgatccg gggtcccggc ttcccttcgg cccggagtcc ggctttctga attctttcga
     1261 aatggtcgag aggaatgccg gaaatgatct tcgtctggcc ggaaagcagg gacgcggttc
     1321 gcgtttccgc ttcctggatg ccatagtatt tcaggacttt aatgggagga ggcggcatcc
     1381 agtaattctt gttgacctcc atctccaccc cgattccccg gttccatttt ttgaagatga
     1441 acggccccga ccccatggga ttggccgtgg tgtgatccag gttgtgggag ccgtagtatt
     1501 gttcatccag gatcgccagg ttgctcatca acgccgggta ggccgggaag ggcgttttgc
     1561 agaggatatc cacggtgtag gagtccacgg ccacggcttt atcaacctgc gcgaaccagt
     1621 tccgttgggg agagacaaac ttcgggtcga ggattcggtt ctgggtgaag gcaaatgact
     1681 tggcggtaag cgggttgccg ttccagaatt tgattccctg acgcatgtag aaacgccaca
     1741 ccgtatcgct cttcgcttcc caaccggtcg ccgcctgggg gaccatttca ttcgtctgtc
     1801 catcccggtc gagggggaag tcaaagatat aggggttcag gagcccattg cccagggctg
     1861 tggtgttctg ggggtccagg gtaacaatgg tctcgctgta ggccgtcccg aattcctcaa
     1921 acaccccttt gtcctggccc caggccaggt tgcgttcgcc ccagatcgga gtcccgaaca
     1981 tcgttgcact catcactccc gtgagcttca aaaaatccct ccggctgatc ggatacttcc
     2041 attccatggc gcatcctcct ttcaaaggct gatctgtggt gttcacaaga gtcagaatct
     2101 attccttctg acggtgagtg ccgcaattgc ttaaaggtaa acctcttcgg gttttctcat
     2161 tctcccgatc cgcacttcgc ctgccccatc aatttggtct cctcatctcc tcatcggctt
     2221 ttccagcagc aggcaggccg ccgtatgacc cccctcccac tctttcaacg ccggctccat
     2281 ttcggcgcat tcgggcagag cttccgggca ccggccgctg aaacggcagc cctgcggggg
     2341 cctgcggggg tctggaggat cccccttcaa aatgatctgc tgcaaagcca gctccggatc
     2401 gggatgggga atggcagaaa gcagggctcg ggtataggga tgcaaggggt tatcgaatat
     2461 ttcctctgtc tcgccgacct ccataatttt gccaaggtac ataaccccga tgcggttgca
     2521 gatgtgccgg gccacggcca ggtcatgggt aatgaacagg taggacagcc cgaatttctc
     2581 ctgcaggtcc atgagcaggt tgagtatttg accctgaatg gacacgtcca gggcggaaac
     2641 cggttcatcg cagatgacaa aagcaggatc aatggccaga gcccgggcga tggcgattct
     2701 ctgctgctgg ccgccggaaa actcatgagg ataacggtcg gcatgcaccg ggctcaagcc
     2761 ggtcagggag aggagttcca tcaccctgtc ccttctttcc ttcccgctcc cctttccatg
     2821 gatctccagg ggctccccga taatttgggc aaccttctgg cgcggatcaa gggaggtgcg
     2881 ggggtcctgg aagatgatct ggacccgttg ataaattgcc ttgagttcgc tccggctgag
     2941 ggaatgaata tcctgcccct caaaatggat ttccccctcg gttaaatcat agatgcgcag
     3001 gatgagacgg gcaacggtgg tctttccgca gccgctctct ccgacgagcc ccagacactc
     3061 ccctttcctg acccgaaagg acaccccgtc aaccgcccga accggcggcg ccatgccgcc
     3121 gaagactatc tccagcagcc cccggtggtc gaaaaaatat ttctttaccc catgaacctc
     3181 tagaatggat tccatcgccc ggattcgtcg cgcaatcttg tagtcttttt gtttcttcgc
     3241 cctttcgccc cttctcttct tccttttcct tgcgccttga gtcttgttcc ttttacctgc
     3301 tcttttggtg acaagccacc cagtgttcct tttccacctc cctccactct ggagtttcct
     3361 gccggcagac atcccggctt tgatcgcagc gcggatggaa ggcgcagccg gggggcatat
     3421 ggatcaagtc gggcatcaac cccgccatcg tttccaccct gcgagtcttc tgattcaaac
     3481 ggataaccga ccggagtaat ccccgggtgt aggggtgcag gggacggagg aacagagtat
     3541 gagtgtcggc gtgctccacc attttgccgg catacatcac atagatgcgg gaacagaact
     3601 gggctacgat tcccaggttg tgggtgatca tgaggaccga catcttccgg gcagattgaa
     3661 ggtccttcag gagctggagg atctgggcct gaatggtcac gtccagcgcc gtcgttggct
     3721 catcggcaat gaccagcgca ggatcgttga tcagggccag ggcaatcaca atgcgctgga
     3781 gcatcccccc gctgtactgg tggggaaagt tctggatgct gatctccggc cggggaatcc
     3841 ccacatctcc catccggcgg atggccaggc ccatggcttc ttctttcctc aggttctggt
     3901 ggagaaagac cgcttccagg agctgatcgc cgatcttttt caagggattg agggcggaga
     3961 tggggtcttg gaagaccatg gcgatctctt tgctgcggat cttccgcacc tccttctccc
     4021 tcttttgccg cagatcttcc cccttccaga agatctgccc ccgggggatc tgtccagggt
     4081 agggaatcaa tcccatgaga gaccgtccca gcatgctttt ccctgccccg ctttccccaa
     4141 cgaggcccac ggtctccccg gtttcctgcc ggaaatccac acggttcacc gcccgcacgg
     4201 tcccccagag ggtggggaag atgacttcca ggtttttgac ttccagaagg ctcatgtggg
     4261 tctgatgctc atcctcaaca aagtaggggc aattcatgaa ttgcccctac ggctgatcca
     4321 cacggatttg ggttcctgct tacgggcagg gattcaattc ttcaggatac atggaggcga
     4381 aaaaagaata tccaaaaaaa ggcaggaccg tcaatgaaaa agatttggca agagggctca
     4441 agaacaagca gaaaaactca gcggttcatc tggaggaggt ggccagccgg catgagcagc
     4501 tccccgatga gaggcgcacc cctacaaact ccaatcttca gcctttaatc cgcgaccgcc
     4561 aatattttca ttcccctttc cgaattctga aatctgcatt tcaaatggct ccttctctcc
     4621 tgagctgggc cacatccttt cccccgatct ggaggtattt ccccaggatg agttcgttgt
     4681 gttccccgac tagcggggaa gtcctctcga tctgacccgg agttttggag agtttgaagg
     4741 ggaagcccga gagtttggcc cgcccgatga tgggatgatc gatctcgacg aagtatcccc
     4801 gttccttcag gtgagggtcc tcgaccacct gctggatgtt gaagatggga gagttggcca
     4861 cccgctcccg gtccagcatc tctcccattt ctttgaccga gtattggacg gtaaccgcct
     4921 cgatttcttt cgacagctcc catt
//
LOCUS       DASPRR010000311         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2325716, whole genome shotgun sequence.
ACCESSION   DASPRR010000311 DASPRR010000000
VERSION     DASPRR010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325716"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..511)
                     /locus_tag="VEL68_11695"
     CDS             complement(23..511)
                     /locus_tag="VEL68_11695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXZ36673.1"
                     /translation="MAETTAVIETKLGNLELKFFPDVAPNHVKNFIDLAKKGFYDGTI
                     FHRVIPGFMIQGGDPNTKNPDRSKHGMGGPGYTVKAEFNDKPHKRGTLSMARAADPNS
                     AGSQFFICVAPASFLDKQYTVFGEVVSGMEAADKIVSAPRDKRDNPNERMEMKVRIVE
                     KQ"
     gene            993..2333
                     /locus_tag="VEL68_11700"
     CDS             993..2333
                     /locus_tag="VEL68_11700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXZ36674.1"
                     /translation="MNEIPLFERPLPLFTRRHFLWLLSMSAAGAAVSCATSPVTGRTQ
                     LMLVSEEEEIQMDRQAAPFQFSADYGPIQDQSLNSYVQQVGKSLAARTHRPQMPYSFR
                     AVNANYVNAYAFPGGSIACTRGILLSLDNEAELAALLGHELGHVNARHTAQQMSKGKL
                     TNTIVGGFSVVAGAVAGGLGQVAGALGSVGAGALLASYSRDNEREADALGMEYMVKAD
                     YGPQGMVGLMEMLQSLSKSKPSAIELMFATHPMSDERYENAVQNSRTKYASAQNLPLN
                     RERYMDHTARLRAMKGTIEELQKGEEAMNGEKMGEAETHFRNALNQTPNDYAGLVMMA
                     TCQLVQKKPGESLKYSEAAKAVYPQEAQAYHLSGFAKLQTRDFAGAYLDFSTNEKVLP
                     GNPNTAFFKGFSLEGMGKKPESAQEYHRYLQVVQEGKYAQHAYARLVEWGYYKR"
BASE COUNT          527 a          682 c          685 g          523 t
ORIGIN      
        1 actgacactg acattgtcac cgttattgtt tttccactat tcttaccttc atttccatcc
       61 gctcattcgg attgtccctc ttatcccggg gggcgctgac gattttgtcc gctgcctcca
      121 ttcccgaaac aacctccccg aagaccgtgt actgcttgtc caggaaggaa gcaggggcca
      181 cgcagatgaa gaactgggac ccagcactgt ttgggtcggc ggcccgggcc atggacaggg
      241 tccctctttt atggggtttg tcgttgaatt ccgccttcac cgtgtagcca ggtcctccca
      301 tcccatgctt ggagcgatcg gggttcttgg tgttggggtc tccaccctgg atcataaatc
      361 ccggaatcac ccggtggaag atggtcccgt cgtagaagcc cttcttggct agatcaataa
      421 agttcttcac atggttgggg gccacatcgg gaaaaaattt aagttccagg ttgccgagct
      481 tggtttcgat tacggcggtg gtctcagcca tttttttcat ctcctcaggg gtgaatttgg
      541 ggtccttggt ttgggcgagg gcgaaactgc tgaccaggac ccagacagcc acccagattc
      601 caatctttaa gaacctcata aaacctccat tgatcccttg ggtcgccgtt tcgggcgtcc
      661 tttattgaat cttcgcacga gcggtctgct gatacaagaa aaaatcgtac accgcccggg
      721 aaacctgggc aatcgcccgc tcccgttcgg ccgctccttt ctccgacgac tgctttccgg
      781 attagaatta tcgttacccg atcctccggg tcggggaaag gggggtagca gctttttagc
      841 tgtttccctt taccggatat tctacaaccg catggagaag gaagggaatg gcgaatcctt
      901 tctgcccgca gaaccggcgc cttctttttt tatccgcatt cttgtggtaa aaatagaata
      961 ttctcgactc gaaagttccg gaaggaggaa gcatgaacga aattccctta tttgaaaggc
     1021 cgcttccttt gttcacccgc cggcattttt tatggctcct gtccatgtcc gccgccgggg
     1081 cggcggtcag ctgcgccacc agcccggtta ccgggagaac ccagctgatg ctggtgtccg
     1141 aagaagaaga aatccagatg gaccggcagg ccgccccttt tcagttttcg gcggattacg
     1201 ggccgatcca ggaccaaagc ctcaacagtt atgtccagca agttggaaag agcctggcgg
     1261 cccgaacaca ccggccccag atgccgtatt cctttcgggc ggtcaatgcc aactatgtga
     1321 atgcctacgc ctttccaggc ggcagtatcg cctgtacccg gggaattctt ctttccctgg
     1381 acaatgaagc ggaactggcg gccctcctgg gccatgagct gggacacgtg aacgcccggc
     1441 acacggcgca gcagatgtct aaggggaagc tgaccaacac gattgtgggc ggcttttccg
     1501 tggtagccgg ggcggtggcc ggaggactgg gtcaggtggc cggggctctg ggcagcgtcg
     1561 gcgccggggc tctcctggcg tcttacagcc gggacaatga acgggaggcg gatgccctgg
     1621 ggatggaata catggtcaag gccgactatg ggccgcaggg gatggtgggt ttgatggaga
     1681 tgctccaaag cctctccaag tccaaaccaa gcgccattga gctcatgttt gccacccacc
     1741 cgatgagcga tgagcgctac gagaacgcag tccagaactc ccggacgaaa tatgcttccg
     1801 cccagaacct tcccctgaac cgggaaaggt acatggatca tacggccagg ctccgcgcca
     1861 tgaagggcac catcgaggaa ctgcaaaagg gggaagaggc gatgaacggc gagaaaatgg
     1921 gggaggcgga gacccatttc cggaatgccc tgaaccagac ccccaatgac tatgccggcc
     1981 tggtcatgat ggccacctgc cagctcgtcc agaaaaagcc gggcgaaagc ctgaaatact
     2041 cggaagcggc caaggccgtt tatccccagg aagcccaggc ctaccacctg agcgggttcg
     2101 ccaagctcca gacccgggat tttgccgggg cctacctgga ttttagcact aatgaaaagg
     2161 ttctgcctgg aaatcccaat acagcattct tcaagggatt ttccctagag ggaatgggga
     2221 aaaaacccga atcggcccag gagtaccacc ggtatttgca ggtggtccaa gagggaaaat
     2281 atgcccagca cgcctacgcg cgcctggtgg aatggggata ctacaagcgc tgaaaggcaa
     2341 tgattcgcct tattccggcc tgctcggaag aagacctttc ccgagcgaga gccctcttcg
     2401 aggaatacgc ctcctct
//
LOCUS       DASPRR010000312         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_224096, whole genome shotgun sequence.
ACCESSION   DASPRR010000312 DASPRR010000000
VERSION     DASPRR010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224096"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VEL68_11705"
     CDS             <1..808
                     /locus_tag="VEL68_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ36675.1"
                     /translation="ENPNAQVREAIRLLGGIQRFVHGGDRVVLLPNPQGTGRGVTTNP
                     DMVAETIRLCLAAGASSVSVSSCHGQEDWRGTGIIEKVEASGGRMKYPDSDRDWAAVA
                     IPNAQARKEVKVIRDALQADVLINMPIFKQHNYTRITGCLKNLMGTNDDNWSFHKGDL
                     YLHQAIVDLASLFSPALCLVDATTILTQGGPFGPGRVIHPKKVYAGKDMVGLDALCCG
                     MLGVKPQEVLHIKLAHESGLGKMDLEGRVIRKTAAVSLQENSDTIPGRTG"
     gene            985..2358
                     /locus_tag="VEL68_11710"
     CDS             985..2358
                     /locus_tag="VEL68_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXZ36676.1"
                     /translation="MPLATLLCIGAGHVGGPTMAVIARKCPEHRVVVADINAAKISAW
                     QSERLPIYEPGLQEVVFSCRGKNLFFSSEVERHIAEADIIFVSVNTPTKSFGQGAGKA
                     ADLQYWEKTARQILQHSRTSKIVVEKSTLPVRTAEAMERILQSQDRSLQFDVVSNPEF
                     LSEGTAIRDMENPDRVLIGARDTPSGGKARQAIVDLYAHWVPRERILTTSLWSSELSK
                     LVANAFLAQRISSINSISALCEKTEADIQEIALAIGMDTRIGPRFLNASIGFGGSCFK
                     KDILNLVYLCEYYHLTEVAEYWEQVVRMNEYQQARFLSRILSSLFNTVAGKRVALFGA
                     AFKANTSDTRESPALQVCRALLEERADVVVTDPHALDNVRVDLGDDARRVTMEPDPYR
                     AAMNAHAIALLTEWTLFSELDYEAIYHSMAKPAFFFDGRNILDHRKLFQIGFNVHAIG
                     KPSLIHP"
     gene            2526..2999
                     /locus_tag="VEL68_11715"
     CDS             2526..2999
                     /locus_tag="VEL68_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXZ36677.1"
                     /translation="MDIRKKTIEGLKVGDTFSLTRAFTEKEVSLFADLTHDYNPVHFE
                     KRFARAKGFKGLICHGLLVASMISEIGGQIGWLASEMSFRFKKPVYPGETVRCHFTLT
                     RRSEKGKAEAEAVFLATDGTVVLEAHLKGIVPGAQEKEVMKAMVAEGDPTNKLKP"
BASE COUNT          735 a          882 c          861 g          724 t
ORIGIN      
        1 agaaaatcct aacgctcagg ttcgagaggc cattcgtctt ctggggggaa ttcagcggtt
       61 tgtgcacggg ggagaccggg ttgtcctcct tccgaacccc caaggaaccg ggcggggggt
      121 caccaccaac ccggatatgg tggcggaaac catacggctt tgtctggcgg ccggcgcctc
      181 ttcggtgagc gtatcttcct gccacggcca ggaagactgg cgcgggaccg gcatcatcga
      241 gaaggtcgag gcctcagggg gacggatgaa atatccggat tcggaccgtg actgggcagc
      301 cgtcgccatt cccaatgccc aggcccggaa agaagtgaag gtgatacggg atgccctaca
      361 ggctgatgtt ctcatcaaca tgcccatctt caaacaacat aactacacgc ggatcacggg
      421 ctgcctcaag aatctgatgg ggacgaatga cgacaactgg tcctttcata agggggatct
      481 ttacctgcac caggccattg tcgacctggc ttcgctcttt tcccccgccc tttgcttggt
      541 cgatgccacg accattctca cgcaaggggg gccctttggc cccggaagag tgatccatcc
      601 gaaaaaggta tacgccggca aagacatggt cggactggac gccttgtgct gcggcatgtt
      661 gggggtaaag ccccaggagg tcctccatat caagctggct catgaatccg ggctggggaa
      721 gatggacctg gagggaaggg tgatcaggaa aacggcggcg gtttctctcc aggagaatag
      781 cgacacaatc ccagggcgca ccgggtgata gcagcccact ttcctaggaa acttttgccc
      841 ttcagtcgaa tttaacgagt gtagctatac accctaagca aggggacttt cccaggtcta
      901 acctttaatc ctttggcatc ctttctggct tatggtacaa tccccctgaa tatttctgcc
      961 aattcttttt ttcccctgcg gaggattccg ttggctaccc ttctctgcat tggagccggt
     1021 catgtgggcg ggcccacgat ggcggtgatc gcccggaaat gtcccgagca tcgcgtggtc
     1081 gtggcggata tcaatgccgc aaaaatctcc gcctggcaat ccgaacgttt acccatatac
     1141 gaacctggtt tgcaggaagt ggtcttttcc tgccggggca agaacctctt cttttccagc
     1201 gaggtggaga ggcacattgc cgaagccgac attatctttg tcagcgtgaa tactcccacg
     1261 aaaagcttcg gccaaggtgc cggaaaagca gccgatcttc agtactggga gaaaacggcc
     1321 cggcaaatcc tgcaacactc ccgcacctct aaaatcgtgg tcgaaaagag cacccttccc
     1381 gtccgtacgg cggaagccat ggagcggatt cttcaatccc aagatcggag tcttcaattc
     1441 gacgtggttt cgaatcctga atttttgtcg gaggggacgg ccatccggga catggaaaac
     1501 cccgaccgtg tgctcatcgg cgcgcgggat actccctccg gggggaaggc gaggcaggcc
     1561 atcgtggacc tttacgccca ttgggtcccc cgggaacgaa tcttaaccac cagtttatgg
     1621 tccagcgagc tttccaaatt ggtcgccaat gcttttttgg cacagcgtat ctcttcgatc
     1681 aattccattt ccgccctttg tgaaaaaacc gaggcggaca tccaagagat tgcccttgcc
     1741 attggcatgg acactcgcat cggtccgcgt tttttgaatg ccagcattgg gtttgggggg
     1801 tcctgcttca aaaaggatat tctaaatctc gtgtacctct gcgaatatta tcatctcacc
     1861 gaagtggccg aatactggga acaggtcgtt aggatgaacg aataccaaca ggctcgtttt
     1921 ctttcccgca ttctttcttc cctgttcaac accgtcgcgg gaaagagggt tgctcttttt
     1981 ggggcggcct tcaaagctaa taccagcgac actcgggaat ccccggccct ccaggtctgt
     2041 cgggcgcttt tggaggagcg ggccgatgtc gtagtgactg acccccatgc tctggacaat
     2101 gtccgcgtgg acctgggaga tgatgcccga cgggtgacca tggagcccga cccttatagg
     2161 gcggcgatga atgcccacgc cattgccctg ctgaccgaat ggaccctctt ttccgaattg
     2221 gattatgaag ccatctatca ttccatggcc aagccggctt tcttcttcga tggccggaat
     2281 attctggacc accggaagct ctttcaaatc ggattcaatg tccatgccat cgggaagccc
     2341 tccctcattc acccgtaaag gttcccttat gcccatcctc gaaggtctac atataacccg
     2401 tcccactctc ctgttggata aaaaccggat gatccgagtg gaaaagggca aggatcccat
     2461 cggcagggcg gagacgaact cggacccaga aggatcttat taatagagac aggaagcagt
     2521 gagttgtgga cattcggaag aagaccatcg aaggattgaa ggtgggagac acgttttccc
     2581 tgacccgcgc cttcacggaa aaagaggttt ctctttttgc ggacctcacc catgattaca
     2641 acccggtcca ttttgaaaaa cgctttgcca gggctaaagg cttcaagggc ctcatttgtc
     2701 acggtcttct tgttgccagc atgatctccg aaatcggagg tcagatcgga tggctcgctt
     2761 cagaaatgag ctttcgtttc aagaagccgg tgtatccggg agaaacagtc cgctgtcatt
     2821 tcacccttac cagaaggagc gaaaagggaa aggctgaggc cgaggctgtt ttcctggcga
     2881 ccgatggaac cgtggttctc gaagcccacc tgaagggaat cgtccccgga gcccaggaaa
     2941 aggaagtcat gaaggccatg gtcgccgaag gagacccaac caataagcta aaaccctaac
     3001 ccacgcaccg ccggccttat ttcgttgttg gacaggaatc cttccattgc cgccaagttt
     3061 tacaggcgca tggctatggg cattgcccag cgcttgaaat acatgaacga agacttcgcc
     3121 gtcctgaaag cttttctttc cagtggaaaa tggagccctt ttttttaagt ccacaatccg
     3181 aaatctgcaa tccgaaatcc aa
//
LOCUS       DASPRR010000313         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_74291, whole genome shotgun sequence.
ACCESSION   DASPRR010000313 DASPRR010000000
VERSION     DASPRR010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74291"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VEL68_11720"
     CDS             complement(<1..185)
                     /locus_tag="VEL68_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36678.1"
                     /translation="MKRSCIVVLAIMTMLLGLGLSAFAAEYPTKPINLVIQYPAGGST
                     DLTARALANGAKKYLGQP"
     gene            complement(318..1289)
                     /locus_tag="VEL68_11725"
     CDS             complement(318..1289)
                     /locus_tag="VEL68_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36679.1"
                     /translation="MKSWTHLWVGVSVIFSLVLGGGITVSAQDFPTKPISFVIPYPAG
                     GSTDLTGRAMANAAKNYLGQPIICENKGGGGGTVGPSLVVSKPPDGYTIGISSGAVTI
                     AYHMGKLNFNPLTDTTNIIRYTTYVFGMVVRADSPWKTMQEFVKYAKENPSKVTYGTP
                     GVGTNPHLAMEELSIITGIQLVHMPFKGGAEAATALLGGHIDAVSDSTSWGPMVDAGN
                     FRLLVTYAPQRMPRYPDVPTLKEAGYDMVYSSPLFLIGPKGMPKPVVAKLHDAFKKSL
                     NDPDYQSILKKYDMSDNYLGPEDLDKAIRQESEQIKRVVQKLGLDKK"
     gene            complement(1372..>2302)
                     /locus_tag="VEL68_11730"
     CDS             complement(1372..>2302)
                     /locus_tag="VEL68_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ36680.1"
                     /translation="NIPELLDGVGLVPVVMGLFGISEVLLNIELKVKREVFTAKVKGL
                     LPTLEDWGKSIWSIARGTVIGFFLGILPGGGAVIASFVSYAIEKRVSKHPEEFGKGAI
                     EGVAAPESANNSAAQGAFIPLMTLGIPSNVVMAMLLGALIIHGVTPGPLLLSQHPELF
                     WGVVASMYVGNAMLLALNLPLIGIWVQLLRVPYSILFPIIILFCLLGAYSINNSSTDV
                     LIMLVFGVIGYFMKKVSLEAAPMVLAFVLGPMMETALRQALIKSKGSFSIFFTRPISA
                     TCLIIAIALMIMPLLPWFRRRRPGATFEQEDVT"
BASE COUNT          537 a          610 c          644 g          511 t
ORIGIN      
        1 ggctggccca ggtatttttt tgctccattc gccaaggccc gggcagtcag gtccgtcgat
       61 ccccctgcgg gatactgaat cacgagattg atcggcttgg tggggtattc ggcggcgaag
      121 gccgacaatc ccaaccccag aagcatggtc atgattgcca ggacaacgat acaactccgt
      181 ttcatcgaaa cctcctttga aaaattccct tcagtattcc gaagaaaaaa gtaccagcct
      241 ctgccgtccc tgtcaagtct atcgggactt caagctgatc ctcttcaatt ttaatccgca
      301 atcagcaatt catttttcta cttcttgtcc aatcccagct tctggacgac tcttttgatc
      361 tgttcggatt cctgtcgaat agctttgtcc aggtcctccg gacctagata gttatccgac
      421 atatcgtatt tcttgaggat cgactgataa tcaggatcgt tcagggattt cttgaaggca
      481 tcgtgcaatt tggccaccac cggcttgggc atccccttgg gaccgatgag gaaaaggggg
      541 gaggaataga ccatgtcgta cccagcctct ttcaacgtgg gcacatcggg gtagcggggc
      601 atccgctggg gggcgtaggt gacaagaagc ctgaaattcc ctgcatccac cataggaccc
      661 cagctggtag agtcagaaac cgcatcgatg tggccgccca gaagggcggt ggcggcttcg
      721 gcaccccctt tgaaaggcat gtggaccagc tggatcccgg taatgatact caactcttcc
      781 atggccaggt ggggattggt ccccacgccc ggcgtcccgt aggtgacttt actggggttc
      841 tccttggcgt acttcacgaa ctcctgcatt gtcttccacg gggaatccgc acggactacc
      901 atgccgaaca cgtaagtagt gtaacggatg atgttggttg tatccgtgag agggttaaaa
      961 ttcagctttc ccatgtgata ggcgatggtg acggccccac tggagatccc gatggtgtat
     1021 ccgtctgggg gtttggagac taccaggctg ggtccgaccg ttcctcctcc tccgcctttg
     1081 ttctcacaga ttatgggctg gcccaaataa tttttggccg cgttcgccat ggcccgcccg
     1141 gttaaatcgg tcgatccccc cgccggatag ggaatgacga aggaaatggg tttggtagga
     1201 aaatcttggg cggaaaccgt gatgccacct cccaacacta ggctaaatat aaccgaaact
     1261 ccgacccaca gatgtgtcca gcttttcata tctcctcctt gtggtaaaac caatccttgg
     1321 atttccgaaa tttgaatggc ccgccagccg gcttttatga tttgggattt attaggttac
     1381 gtcttcttgt tcgaaggtcg cccccggccg gcggcggcgg aaccagggaa gcaggggcat
     1441 gatcatcaag gcaatggcaa tgatgaggca ggtggccgaa atcggccggg tgaagaagat
     1501 ggagaaactt cccttggact tgataagcgc ctggcggagg gcggtttcca tcatgggtcc
     1561 gaggacgaaa gccagcacca tgggagcggc ctccagggaa accttcttca taaaataccc
     1621 gatgactccg aaaaccagca tgatcaatac atccgtggag ctgttgttga tggagtaggc
     1681 ccccaggagg caaaagagga tgataatcgg aaaaaggatt gagtagggga ctctcaggag
     1741 ctgaacccat atcccgatga ggggaagatt caaagccagg agcatggcat ttcccacgta
     1801 catactggcc accacgcccc agaagagttc ggggtgttgg gagagaagaa gagggccggg
     1861 agtcaccccg tggataatca gggctccgag cagcatggcc atgaccacgt tggaaggaat
     1921 ccccagggtc ataaggggaa tgaaagctcc ctgggccgcg gaattgttcg ctgattccgg
     1981 agcggcgacc ccctctatgg cccccttgcc aaactcctcc ggatgcttgg agacgcgctt
     2041 ttcgatggca taggaaacga aggaggcaat gaccgctcca cccccgggga ggattcccag
     2101 gaagaaaccg attaccgtcc ctcgagcgat cgaccaaatc gatttgcccc aatcctccag
     2161 agtgggaagg agccctttga ctttggcggt gaagacttcc cgtttgactt tgagctcaat
     2221 gtttaaaagg acttcggaaa tgccgaagag gcccatgacc acgggaacga ggccgacgcc
     2281 gtccaaaagt tccgggatat tg
//
LOCUS       DASPRR010000314         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_557037, whole genome shotgun sequence.
ACCESSION   DASPRR010000314 DASPRR010000000
VERSION     DASPRR010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557037"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VEL68_11735"
     CDS             <1..900
                     /locus_tag="VEL68_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ36681.1"
                     /translation="DNLYDATRPDYKIAQRAKRTASALDNALHAEHGLAYHIQTVVEG
                     RSHSFLFDFAADFRGVKRNMDLLKIDLKNIEALAVSHDHWDHQAALLELLAAKKDEIP
                     GGVPLYVGEQFFVGTYSRVPDGKVISLLALKREDVERSGVVKIRETKQPTPIVPGAYS
                     TGKVAQVTDYEKIPPNFVARRGDQWVQEDFPGEQAIVLNAKGKGLIVLSGCAHRGIVN
                     TVMHAQKMTGIEKVHAIIGGFHLTGAKPEVIQKTIADIKAVRPDYIVPTHCTGFEAIS
                     AFAREMPDQFILNTAGTKYTISQ"
     gene            1041..1496
                     /locus_tag="VEL68_11740"
     CDS             1041..1496
                     /locus_tag="VEL68_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ36682.1"
                     /translation="MRTILLVGFLLVFILSGQALAQPLAKHLIDSKPGEAVLLSLGEW
                     FAAHPLKEGKVKMEKVYESPRDTINLAIIKDATIGRHLHTNVDEIVYVYKGTGEMNIN
                     GKWVPVKAGDLHVCPRGVAHSTRAGAEELWVISIFSPAMPAAGDRVTFE"
     gene            1984..>2625
                     /locus_tag="VEL68_11745"
     CDS             1984..>2625
                     /locus_tag="VEL68_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36683.1"
                     /translation="MKIIGIFIVCLFVFLGNCLAWADATVESSIKSSGFKGMGAFEGT
                     TSRKYQGEKMWDSTSSKFTGAIMSRVAGGSESTTITRVDKGVHWILDPKNKTYVERPI
                     EAIKMGEPGKEKPEQEKSKVRVTKSEFSVKKTGASETINGFPCEEYLVTWLLEMEDME
                     TKAKSRSTMTNNLWTTPETATIQKVQAEERAFQKAYAQKVGLQISPEEVKQMGI"
BASE COUNT          683 a          634 c          737 g          571 t
ORIGIN      
        1 gacaatttgt acgatgcgac caggccggat tataagatcg cccagcgcgc gaaacgcact
       61 gccagtgccc tcgacaatgc cctccacgcc gagcacggcc tggcttatca catccagacc
      121 gtggtcgaag ggcgatccca ttcctttttg tttgattttg ccgccgactt caggggggtc
      181 aaaagaaata tggacctgct gaaaattgac ctcaaaaata ttgaagcctt ggccgttagc
      241 catgatcatt gggatcatca ggccgcgttg ctggaactgc tcgcggcgaa aaaggacgaa
      301 attcccgggg gagtccccct ttacgtgggg gaacagttct ttgtagggac ctattcccga
      361 gtccccgacg gcaaggttat cagccttttg gcgctcaagc gggaggatgt tgaaagatcg
      421 ggggtggtga agattaggga gaccaaacag ccaaccccga ttgttccggg ggcctattcg
      481 accggaaagg tggcgcaagt tactgattat gagaagatcc cgcccaattt tgtggccagg
      541 agaggtgatc agtgggtgca ggaggatttt cccggagagc aggccatcgt gctgaatgcc
      601 aaggggaagg ggctgatcgt cctgtctggc tgcgctcacc gggggatcgt gaacacggta
      661 atgcatgcgc agaaaatgac cgggatcgaa aaggttcatg ccatcatagg aggatttcat
      721 ttgaccgggg cgaaaccgga agtgatccag aagacgattg ccgatatcaa agcagtgcgg
      781 ccggactata tcgttcccac ccattgcact ggattcgaag ccatctcggc ttttgccagg
      841 gagatgccgg accagtttat tttgaatacg gctgggacga aatacacaat ttctcagtaa
      901 ctgccaccga ttaaccctca gccgtcctta tatttagacg ggttcatttt tgattcggcc
      961 aagcggagaa acacagcgag gagagagaaa cgcccgggga agggcaaata aaaatcaaaa
     1021 gaagaaagga gaacattaaa atgcgtacga ttctactggt gggattcctg ttggttttta
     1081 ttctttccgg tcaggcttta gcccagcctc tggctaagca cttgatcgat tccaaacccg
     1141 gggaagctgt actccttagc ctcggcgaat ggtttgccgc ccatcccttg aaggagggaa
     1201 aagtcaagat ggagaaggtc tatgagtcac cccgggacac cattaacctg gctataatca
     1261 aggacgccac gatcgggcgc catctccaca cgaatgtcga tgagattgtc tatgtttaca
     1321 agggaacggg ggaaatgaac atcaatggca agtgggttcc ggtaaaagcc ggagaccttc
     1381 atgtctgccc gcggggcgtt gcccattcca cccgggccgg ggctgaagaa ctttgggtca
     1441 tttccatctt ttccccggcc atgcccgcgg ccggcgaccg ggtgacgttc gaatgataga
     1501 tgacgaaatc tctttttaga taggggcggt tcgccttcgg cccgagctca ggccgagggg
     1561 cgaaccgccc ctgcccaggg tcagtgcaag aaactctcca ttccagtccg gtttctccaa
     1621 tcatacaccc gtaaaatgga tagggactta tagcccgcta gaagagaata acctggattt
     1681 tcttttcccg gtctattgca tcaaaaatat ggcttctgcg gattcctgac gaatgtctgc
     1741 atggaaggcg ccgcgaggag ccttcccccc cttcctggtt tacggattgg ttctacgttt
     1801 cccgccggga atatttatcc tcgccgcttg aaaaggtatc ctaattttat ttacggaatc
     1861 cttgcaagaa gaggggaggg cggacgggta tttctttccg acttgactat aaaaatattg
     1921 accttctaat ttgatagggc ctatcataga agaaagccaa cctaatcaaa ggaggaactt
     1981 gggatgaaaa ttataggtat atttattgtg tgcttattcg ttttcctcgg aaattgtctg
     2041 gcgtgggcgg acgcgacggt cgaatcttcg atcaagtcca gcggattcaa agggatgggg
     2101 gcctttgagg ggacaacctc caggaaatat cagggggaga agatgtggga ttcgacctct
     2161 tccaagttca ccggggcgat catgtccagg gttgccggcg ggagcgaaag caccaccatc
     2221 acccgggttg acaagggagt ccactggatt ctggacccca agaacaaaac ttatgtggaa
     2281 aggccgatcg aagccataaa aatgggagag ccggggaaag aaaagcccga gcaggaaaaa
     2341 tccaaggtgc gggtgaccaa atctgaattt tcagtgaaga aaaccggcgc ctccgagacg
     2401 atcaacgggt tcccctgtga agaatacctg gtcacctggc tattggaaat ggaagatatg
     2461 gaaaccaagg ccaagtcccg tagcacaatg accaataatc tctggaccac tccggagacc
     2521 gcaacgatcc aaaaggtgca agcagaagag agggcgtttc aaaaggccta cgcccagaag
     2581 gtggggctgc agatatcacc ggaagaagtc aaacagatgg gaatc
//
LOCUS       DASPRR010000315        13065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2326805, whole genome shotgun sequence.
ACCESSION   DASPRR010000315 DASPRR010000000
VERSION     DASPRR010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13065
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326805"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1020
                     /locus_tag="VEL68_11750"
     CDS             76..1020
                     /locus_tag="VEL68_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase-like protein"
                     /protein_id="HXZ36684.1"
                     /translation="MILEAFRRLKQAGVLGVNGRNAEYIMRCNPRSFFPLVDDKVLTK
                     KLAESFEIPAPMLYYVVDRYGDVPGFQGALRGICQFVVKPARGTGGSGIVLIRDCTEK
                     GYVTQSGELISPQNLFYHISDILSGIYSLSGLEDRAILEALIHPDPVFDAISYQGVPD
                     VRIVVYRGVPIMAMVRLPTKASDGKANLHRGAIGAGIELNTGITITAVHRSRVVSTHP
                     DTGNPVSGTQVPYWDKMLWIAARSFEMTGLGYIGVDLVIDQDRGPLLLELNARPGLAI
                     QLANRKGLKDRLDKIDQAPPEVLASAEARVQWAKEAFE"
     gene            1225..1926
                     /locus_tag="VEL68_11755"
     CDS             1225..1926
                     /locus_tag="VEL68_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXZ36685.1"
                     /translation="MNILFFGPNGSGKGTQGAIVQKQYNIPHIESGVIFRENISKGTD
                     LGKKAKSYIDRGELVPDDITIPMILDRLKQPDCAKGWLLDGFPRNVNQAKKLDDALNA
                     AGLNLSYIIEIVLDRKMAKDRIMGRRLCVNDNNHPNNVFIEAIKPAVKGGKQVCRVCG
                     GELKTRSDDQDGNAIDKRHAIYYNTQDGTLAAVNYFKDKAKRDKGALKIIELDGRPGV
                     KEVSEALLKKLGIKK"
     gene            2125..2850
                     /locus_tag="VEL68_11760"
     CDS             2125..2850
                     /locus_tag="VEL68_11760"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXZ36686.1"
                     /translation="MIARMKPRIGITLELSEKAEKRTSFLDLAYAECVKDAGGLPLHF
                     PPLPSVEFIPEALSLIDGLILTGGADINPSYYREEVSAPVVLSPDQRTDFDLALFQAA
                     VSGDKAILAICHGMQIVNVALGETLYQDIPTQVSGAIEHRGKKDSSPARHGVTVEAGS
                     RLAGIMDGTLRFEICSTHHQAIKDLGKGLRVVARSPDGIIEGIELPGHPKVLGVQWHP
                     EKDPQSEATRRLFRALVEAAFVS"
     gene            3236..4426
                     /locus_tag="VEL68_11765"
     CDS             3236..4426
                     /locus_tag="VEL68_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXZ36687.1"
                     /translation="MLYRRLGKTGCDVSVLGFGCMRLPVRDAHKPTDFFDPAKAIDEE
                     KAIELIHYAIEQEVNYFDTAYVYHGGKSEPLLGKAVREYRKKVYLATKLPAWIIKGKE
                     DFEKVLQEQLLRLETDFVDFYLLHGLGRESWQKMKEMGALDFLERVKAEGRIRFAGFS
                     FHDDTRTFKEIADAYDWALCLIQHNYYDRNYQAGREGLEYAAAKGIGVVIMEPLRGGK
                     LAQRIPQEVQGLWDSSPGKRTPAEWALRWVWNHPGVSTVLSGMNSMEQLRENLRVAQE
                     ALADSLKAEELALIDRVTEAYRKLLRVDCTSCGYCLPCPRGVNIPHNFTMYNDLFAFQ
                     APETSLMLYQRWTSPEQRAANCADCGECEEKCPQHIPIAQEMKKVHEALGGRQDSGKG
                     PINP"
     gene            4597..5586
                     /locus_tag="VEL68_11770"
     CDS             4597..5586
                     /locus_tag="VEL68_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXZ36688.1"
                     /translation="MPTPSTPFKIAAVQAAPMFLNREATVEKACRLISEAAHQGARLI
                     VFPESFIPTYPDWVWAVPPGRERILNQLYADFLANAVEIPGAATETLAQAARKAGAYL
                     VMGVTERDTTASGASLYNTLLYFSPEGNLMGKHRKLVPTGGERLVWAQGDGSTLEVYD
                     TPLGKIGGLICWENYMPLARYTMYAWGTQIYIAATWDRGEPWLSTLRHIAKEGRVYVI
                     GCCTALRSADIPDQFEYKDKFYSGSRDWINEGDSAIVNPDGEFIAGPVRMKEEILYAE
                     VDPAQMRGPKWMLDVAGHYARPDVFELIVHRKSHPMIQIAEDRQEAEGEKGEV"
     gene            complement(5593..6366)
                     /gene="folE2"
                     /locus_tag="VEL68_11775"
     CDS             complement(5593..6366)
                     /gene="folE2"
                     /locus_tag="VEL68_11775"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HXZ36689.1"
                     /translation="MIDIQSQRDHRRVGINKVGVKNIEYPITVLDKKQRVQHTVGKVN
                     MYVNLPHHFKGTHMSRFVEILNEYRGTINIKAIATILDKVKEKLNAESAYLEVEFPYF
                     IEKRAPVSGAKSLMGYTCQFFASLTNGHHNLIVGITVPVTTVCPCSREISRKGAHNQR
                     SLVSVRVTFRKFFWIEDLIRIVENSASAAVFSLLKRGDEKFLTEKAYDNPMFVEDVVR
                     SIALRLNSHKNITWFSVESENLESIHNHNAYAYVEKESR"
     gene            complement(6363..6740)
                     /gene="queD"
                     /locus_tag="VEL68_11780"
     CDS             complement(6363..6740)
                     /gene="queD"
                     /locus_tag="VEL68_11780"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HXZ36690.1"
                     /translation="MYEVKIKADFSAAHNLREVGGKCESLHGHNFAVEVAVESESLDE
                     AGLVIDFRLLKAKTKEVLDTLDHQYLNELPFFRNQNPSSENLAAYIFSEVSRRIDQGP
                     RRVKRVSVWESETSRATYRRSGK"
     gene            7072..7869
                     /locus_tag="VEL68_11785"
     CDS             7072..7869
                     /locus_tag="VEL68_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXZ36691.1"
                     /translation="MLIGMMNNPQIDLLGEIAWARQNSFEFLDLSLEPSAAHPRQVKV
                     RPTRRALKAANLDIIGHTAYYLPWASPFETLRKAALKEMRSTLEVFAELGASKVTVHP
                     DKSIPFALGSKGVWEKNLESLEAIESMAAPLGITILLENMDRTFNRVDQIRNALARMP
                     GLGFHLDVGHANLNVEKNRTEEFLKAFHDRLAHVHLSDNFGKSEDLHLPLRAGTIPWP
                     KIIGLLKKSGYDGTITLEVFSQERKYLLLSRDILRQLWGEKNAVSSQ"
     gene            8076..9170
                     /locus_tag="VEL68_11790"
     CDS             8076..9170
                     /locus_tag="VEL68_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36692.1"
                     /translation="MEPVDPERAGRSASLPPLSLLLVHSMDREKVERIKEEEKVPVIV
                     VFPARGDEELLTLEREVQILQPLMGRIIDEIWIAFGGSEHDDIPLMARKYGAKVFHAH
                     RNGMNDARRGDSGKGLSMRGLLYHLVADRQLTDPRAIIQFIDSDIKEGFFNPRWVIDP
                     AGALLWFKKIEVAKIVYHRPFGGRLNAFITPFLSVFDHPALNPLKNLLYFLSGEIAGT
                     LKFWLSVPFKQNFGIEMKILTSLALNKVRLRQNRDDLSHVLQVFMGEMDHKHSALRST
                     ARVKGLDAMAKEVFQSFLEDLTAEGLIRMDKGLCFSNRLQVSTVTPAAEGGEEMGTPS
                     RLNLPSNEKTFFPLKDLPEIQARCPWLKAG"
     gene            complement(9194..9904)
                     /locus_tag="VEL68_11795"
     CDS             complement(9194..9904)
                     /locus_tag="VEL68_11795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ36693.1"
                     /translation="MLTVNFRTFPLEKGDLVLDAGCGEGRHTFECFRHDCRILGMDLS
                     HHSLLKAKYVLGQMEKKKESKGSVLLLRGDALRFPFADGSFDKIICAEVIEHVDDDRH
                     GVATLARILSEGGKIAITVPTRMTEHLYDWLSEEYFQTPGGHVRKVIPRKLAGYMEEN
                     NLRVYAVGFAHAFHSPYWMLRCLSGLHRENAPIPSFYKKLLYYSLFSKPLRFLEHVCN
                     YFFPKSIILYAQKGNHRD"
     gene            complement(9891..11126)
                     /locus_tag="VEL68_11800"
     CDS             complement(9891..11126)
                     /locus_tag="VEL68_11800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ36694.1"
                     /translation="MKIGLLSYRGNPYCGGQGIYLTYLANELVKLGHEVHVIVGPPFP
                     LFLNGSFVHPIENFNFFGKKTDFLPSGNPFKIFSPLNFYEFASSRFGVFPEIKAFSFR
                     AYFKLRELVRHHRFHILHDNQCLGYGQVLWKTLGVPVVATIHHPLSIDRATWFENPST
                     LKQKVKRVLYYPLLMQGWVSRRMDRIITVSHDSAREITDAFGVPPEKIRVVYNGLDSE
                     EFAPLPGWTKKTGNLIFVGNSEDRKKGLLYLLQSLVYLPEEVTLTVVDGGAPQRAFGP
                     LLTEKYRIAHRVHFTGKIGPGDLVRHYNNAEVAVVPSLYEGFGFPAAEAMACELPVVA
                     SSAGALPEVVGNDGAGVLVPPRDAKALAEAILRFLRDPDLRRKTGKAARKRVLKLFTW
                     KNAARQMVEVYQEAIDAHG"
     gene            complement(11239..12462)
                     /locus_tag="VEL68_11805"
     CDS             complement(11239..12462)
                     /locus_tag="VEL68_11805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ36695.1"
                     /translation="MNICLLSYRGNPYCGGQGVYLYFLSRELARMGHSPTVLVGPPDP
                     WPMPWARTLPVESLNLWGVRKNFLPQVSPWRIFRPLNFFEFAATRFGFFPEMLIFSVR
                     ALRALEKIFPREGFQVFHDVQSLGYGLLLVKRFRRPLVTTVHHPLTIDFQHSLERDRN
                     FKEKYYTVVFYPLGMQGRVIRRCDRVLTSSRETARQIEEAFRVSRDRIRNVANGLDTE
                     FFRPGNGTPRGSQRVLFVGNTDDPKKGARYLLQALTLLPEKVNLTIVDEGGPAKTYAP
                     DLVQKLGIFRRVTFTGKLSPENLRREYHSAQVTVVPSLYEGFGLPAAESMACGTPVVV
                     TSAGALPEVVGEDGAGILVPPRDAKALAMAIREVLEDRGRRERMAKAGRERVENLFSW
                     RKVAERTVEVYKELL"
     gene            complement(12472..12753)
                     /locus_tag="VEL68_11810"
     CDS             complement(12472..12753)
                     /locus_tag="VEL68_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36696.1"
                     /translation="MADTSRVWGGFFCVAMVIAAVVFLWGISAQSYWALAIPVMIGCL
                     GVLAMGFWIGWTILTIKTAPPAAELPSPPSGEAKKPSPTGSSEPEKGIP"
     gene            complement(12755..>13065)
                     /locus_tag="VEL68_11815"
     CDS             complement(12755..>13065)
                     /locus_tag="VEL68_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36697.1"
                     /translation="VWRREIPPPHIWEVPRDHTFKFDYPTILRLANKYLRMESVRGVS
                     LLWGFPRWSSFLQRIPQPMALILLRGLDKIANWVPDWSDVLILVGRPHSPLPAKERSS
                     "
BASE COUNT         3088 a         3483 c         3766 g         2728 t
ORIGIN      
        1 ccaaatcctt ctggggaggt ataccggtta tcgcttgtcc gaacttttca ggtttcgtgg
       61 ggtggggggt tcttcatgat ccttgaggcc ttccgccgct tgaagcaagc cggggtattg
      121 ggggtgaatg gcaggaacgc tgaatacatc atgcgctgca atccgcgatc attcttccct
      181 ctcgtggatg ataaagtatt gactaaaaag ttggcggaat cttttgaaat tcctgctccg
      241 atgctttact acgtggttga tcggtatgga gacgtaccgg gcttccaggg agcccttcgc
      301 ggcatttgtc aatttgttgt gaagcctgcc cgggggaccg ggggaagcgg aatcgtcctg
      361 atccgggatt gcacggagaa ggggtatgtg acccagagcg gggagctcat ctctccgcag
      421 aacctctttt atcatatttc cgatatcctt tcgggaatct attccctttc cggtcttgaa
      481 gaccgggcga tcctggaagc cctgatccat cctgacccgg tatttgacgc aataagttac
      541 cagggagttc ccgatgtgcg gatcgttgtc tatcgggggg tgccgatcat ggccatggtg
      601 cgccttccga ccaaagcctc ggatgggaag gccaacctcc atcgcggggc catcggcgcg
      661 ggaatcgaat tgaatacggg gataacgatt acggccgttc accgctccag ggtcgtttca
      721 acccatccag ataccgggaa tccggtaagc gggacccagg ttccttattg ggacaagatg
      781 ctgtggatag ccgcccgttc ttttgaaatg accgggctgg ggtatatcgg agtggacctg
      841 gtaatcgacc aggaccgggg accgcttctc ctggagctca atgcgcgccc ggggctggcc
      901 attcaactgg ccaatcggaa ggggctcaag gatcggctgg acaagataga ccaagcccct
      961 cctgaggttc tcgcctcggc ggaggccagg gtccaatggg ccaaagaggc tttcgagtaa
     1021 aaccgttgct gattgcacaa tctttgttgc tggttgctgg ttccttgtta ctggttaaaa
     1081 gaaaagaaat gatggagcaa aaaggaacga gtaaccggta accagcaact agcaaccaga
     1141 aaccagtagg gagcaacaaa ttcttgattt ttccattgaa tctgagttaa agatggtcag
     1201 gaggagggaa gaaaggagaa agggatgaac attctttttt tcggccctaa cggaagcggg
     1261 aaaggaacgc aaggggcgat cgtgcagaaa cagtataaca ttcctcatat cgagtccgga
     1321 gtgatctttc gggaaaacat ctcgaaaggc acggatctgg ggaaaaaggc caaaagctac
     1381 attgaccggg gagaactggt gccggatgat attaccatcc cgatgatcct ggatcgtctg
     1441 aaacagcccg attgcgccaa ggggtggctc ctggatgggt ttccgcgcaa cgtcaaccag
     1501 gctaaaaagc tggatgatgc cctgaatgca gcggggttaa acctgagtta tataattgaa
     1561 atcgtcctgg accggaagat ggccaaggac cggatcatgg ggcggcggct ctgcgtgaat
     1621 gataacaacc atcccaataa tgttttcatc gaggccatta agccggcggt aaagggcggg
     1681 aaacaggttt gccgggtctg cggtggggaa ctgaagaccc gttcggacga ccaggatgga
     1741 aatgccatcg acaaacgcca cgccatttac tacaacaccc aggacggaac cctggccgcg
     1801 gtaaattact tcaaggacaa ggctaaaagg gacaaaggag ctttgaaaat catcgagctc
     1861 gacggtcgtc cgggagttaa agaagtgtcg gaagccctat tgaaaaagct cgggataaag
     1921 aaataagtgt cagtgcttcg tgtcagtaaa gccaagaaat gtatcaattg gaccactctg
     1981 acccaaatta tgatgatctc gtaaaaaggc gaaaccccca aaatatgtca ttccggcgaa
     2041 agccggaatc caggtatttc aaataattct ggaccccggc tttcgccggg gtgacatttc
     2101 aagagacttt ttacgagatc atcaatgatt gctcgcatga aaccccgtat tggaattact
     2161 cttgagctca gtgaaaaggc ggaaaagagg acaagttttc tcgaccttgc ctatgccgag
     2221 tgcgtgaagg acgcaggggg gcttcccctg cattttcccc cgcttccttc agtggaattc
     2281 attccggaag ccctttccct gatcgacggt ctgatcctga ccggaggggc ggacatcaat
     2341 ccatcctatt accgggaaga agtctcagct cctgtggttt tgtccccgga ccagaggacc
     2401 gatttcgact tagccctctt ccaggcggct gtgagtgggg acaaggccat cttggccatc
     2461 tgccacggga tgcaaatcgt aaacgttgcc ctgggggaaa ctctttacca agatatcccc
     2521 actcaggttt ccggagcgat tgaacaccgg gggaaaaagg attcgagtcc tgcccgacat
     2581 ggggtgacgg tcgaagcggg ctcaaggctg gcgggaatta tggatggaac gctccggttt
     2641 gaaatctgca gcacccacca ccaggcgatt aaagacctgg gaaaaggcct gcgggtcgtt
     2701 gctaggtctc cagacggaat catcgagggg attgaacttc ccggacatcc aaaagtccta
     2761 ggcgttcaat ggcaccccga aaaggacccg cagagcgaag ccacccgccg tctttttcgg
     2821 gccttggtag aggcggcctt cgtcagctag ttgagaaaat ggaaaaataa atagcatcca
     2881 aatggtattt gggttgcatt ggtgacatgg atactaccat tcggaatctt gataagtcgt
     2941 gccgcgctca cggggaaaac catcggagag gtggtcaatg aagtcatccg gtgctatctt
     3001 gcccggccct atcctagggg ggatagaaat cggcccattc tcttcaagaa ggtatcccca
     3061 attccgatcg cctcagcctt tccctaagct tcaaatttaa aaacctaaac atggcactct
     3121 gaatttgcct ttctctccgc tttgcaaagg ttccggggcg aggtagaatt tatgcggagg
     3181 ggtgagctga agctatgacc cctcgatagg ataaacgaag gaggaatttt caggaatgct
     3241 ctaccgaaga ctggggaaga ccggctgcga tgtttccgtt ctgggctttg gttgcatgcg
     3301 cctgccggtc agagacgcgc ataagccgac cgattttttc gaccctgcca aggccatcga
     3361 cgaagaaaaa gccatcgagc tcattcatta cgccatcgaa caggaggtca attacttcga
     3421 tacggcctat gtctaccacg gagggaaaag cgagccttta ttgggcaagg ccgtccggga
     3481 ataccgcaag aaggtctacc tagccaccaa gctgcccgcc tggatcatca agggaaagga
     3541 agatttcgag aaggtccttc aggagcagct tctccggctg gagaccgatt tcgtggactt
     3601 ctatcttctc cacgggctcg gccgggagag ctggcaaaaa atgaaagaaa tgggagcgct
     3661 cgatttcctg gagcgggtga aggctgaggg aaggatccgc ttcgctgggt tttccttcca
     3721 tgacgataca cggaccttca aggagatcgc ggatgcctat gactgggccc tctgcctgat
     3781 ccagcacaac tactacgacc ggaattacca ggcgggcagg gagggactgg aatacgcggc
     3841 cgccaagggg attggggtgg tcatcatgga gccgttgagg ggaggaaagc tcgcccaaag
     3901 aatcccgcag gaagtgcagg gcctttggga ctcctcgccc ggcaagagaa cgcccgccga
     3961 gtgggccttg cgctgggtct ggaaccaccc gggagtttca accgtgctga gcggaatgaa
     4021 ctccatggag cagctgaggg agaacctccg ggtcgcccag gaagcccttg ccgattctct
     4081 caaagcggaa gaattggccc tgatcgatcg tgtgaccgag gcttaccgga aattactccg
     4141 ggtcgactgc acctcctgcg gctactgtct cccctgtccc cgaggggtta atatccccca
     4201 taacttcacc atgtacaacg accttttcgc cttccaggcc cccgagacca gtctcatgct
     4261 gtaccagcgc tggacctccc ccgaacagcg ggcggcgaac tgcgccgatt gcggtgagtg
     4321 cgaagaaaag tgcccgcagc acatccccat cgcccaggag atgaagaagg ttcacgaggc
     4381 actgggcggg cggcaggaca gcggaaaggg tcccatcaat ccctgagctg attcggcaat
     4441 attgggggca ggcctgcaat tttggagttt ggatgttgat tttataggct ataaagaatc
     4501 aaatccgtga atggttttga aaaatagctg gaagtccttg gggcgatcgt ggagtataat
     4561 gaacaaaaaa ggaaagcaaa gtctaaggag tcccaaatgc caaccccctc taccccattt
     4621 aaaattgctg ccgttcaagc tgcacccatg tttttgaacc gggaggcgac cgtggaaaag
     4681 gcctgccggc tgatctctga ggccgcgcat caaggcgccc gactcatcgt atttccggaa
     4741 tccttcatcc ccacctatcc cgattgggtg tgggcggttc ctccgggaag ggaaagaatc
     4801 cttaaccagt tgtacgctga ttttctggct aatgccgtgg aaattcccgg cgcggcgacc
     4861 gagacactgg cccaggcggc tcgaaaggct ggagcctatt tggtcatggg cgtcaccgag
     4921 agagatacta cggccagcgg ggccagcctc tacaacacct tgctttattt cagtcctgag
     4981 ggcaacctga tgggcaagca ccgtaagctg gttcccacgg ggggcgagcg actggtttgg
     5041 gcccagggtg acggcagcac actggaggtc tacgacactc ctctgggaaa gatcggcggg
     5101 ctgatctgtt gggaaaacta tatgcctctg gcccgctaca cgatgtacgc ctggggcacc
     5161 cagatttata tcgccgcaac ctgggaccgc ggggaaccgt ggctttccac cttgcggcat
     5221 atcgccaaag agggacgggt ctacgtcatc gggtgctgca ccgccctgcg cagcgcagat
     5281 atccccgacc agttcgagta caaagacaaa ttctattccg ggtcccggga ttggatcaac
     5341 gaaggggaca gcgccatcgt gaacccggac ggggaattca tcgccggacc ggtccggatg
     5401 aaagaagaga tcctttatgc cgaagtggac cccgcccaga tgaggggtcc caagtggatg
     5461 ctggatgtgg ccggccatta tgcccggccg gacgttttcg aactgattgt ccatcggaaa
     5521 tcccacccga tgatccaaat cgcggaggat aggcaggaag ctgaaggaga gaagggggag
     5581 gtctaaaaag aactatctgc tttctttttc cacgtacgca taggcgttgt gattgtggat
     5641 ggattcgagg ttttccgact ccacgctgaa ccaggtaata tttttgtggg agttgaggcg
     5701 gagggcgatg ctgcgcacca cgtcctccac gaacatggga ttgtcgtaag ccttttcggt
     5761 gagaaacttc tcatctcccc gcttaagcag ggaaaagacc gccgcgctgg ctgaattctc
     5821 cacgatccgg atcaggtctt caatccaaaa gaatttgcgg aaagtcaccc tgaccgaaac
     5881 caggcttcgc tggttgtggg cccctttcct gctgatctca cgggagcaag ggcataccgt
     5941 tgtgaccggc acggtgattc ccacgatcag gttatgatgc ccgttggtca aggaagcgaa
     6001 gaactggcag gtatacccca taagactctt ggccccggaa acgggagccc gtttttcgat
     6061 gaaatagggg aactccacct ccaggtaggc cgattcggcg ttgagctttt ccttcacctt
     6121 gtccaggatg gtggcgatgg ccttgatatt gatcgtgccg cggtattcgt tgaggatttc
     6181 cacgaatcgg ctcatatggg ttcccttgaa gtgatggggc aggttgacgt acatgtttac
     6241 cttgcccacg gtatgttgaa cccgttgctt cttgtcaaga acggtgatgg ggtactcgat
     6301 gttcttcact cccaccttgt tgatccccac ccggcgatga tccctctggc tttgaatgtc
     6361 gatcattttc ccgaccttcg gtaggtggcc cgggaggtct ccgactccca gacggaaacc
     6421 cgcttaaccc gtcggggtcc ctggtcgatc cggcgggaga cctcgctgaa aatataagcg
     6481 gccaagtttt ccgacgacgg gttctggttg cggaaaaagg gaagctcgtt caggtattgg
     6541 tggtcgagag tatccaggac ttccttggtc ttggccttga ggagacgaaa gtcgatgact
     6601 aacccagcct catccaacga ctcggattcc acggccacct cgacggcgaa gttatggccg
     6661 tgaagagact cgcatttccc ccctacctca cgaaggttgt gagcggcgga aaaatcggct
     6721 ttgattttaa cttcgtacat gatcggaact ggcaacccaa aaaagaaaag cgcttagggc
     6781 atagcgaaag caagaaaatg aaatcaatga aggggatgca ttcaacttac tcgattaagg
     6841 ccacgttgtc aacttcacaa aattgggggg ggattggaaa tagggaaggg aaggccaatg
     6901 ccccatgggc gctgtatctc gaaacaaaga aaagggcttg agtttgccca aagaaggacg
     6961 gtttgggagc gaatagtacg cattcaaaca tcttgacact ttccttttct gctggtagtc
     7021 tacgcgggaa atgcggtggg aaaacggagc aggcagggag gagcgtaggt attgcttatc
     7081 ggcatgatga acaaccccca aattgatctg ctgggcgaga tcgcctgggc acgccagaac
     7141 agctttgaat ttctggattt aagcctggag ccgagcgcgg cgcatccaag gcaggtaaag
     7201 gtaagaccga cccggcgagc cctgaaggca gcaaacttgg acatcatcgg ccacaccgcc
     7261 tattaccttc cctgggcttc tcccttcgag actcttcgca aggcggccct gaaggagatg
     7321 cgctcgaccc tggaggtgtt cgccgaactg ggggcatcca aagtaaccgt ccatccggat
     7381 aaatcgatcc cttttgccct gggttcgaag ggcgtctggg agaagaacct ggagtcattg
     7441 gaggccattg agtccatggc cgctcctttg ggaattacga ttctgctgga gaacatggac
     7501 cgaaccttta acagggtgga ccagattcgg aacgccttgg cgcgcatgcc cggtttgggt
     7561 tttcacctcg acgtggggca cgccaacctg aatgtggaaa aaaaccggac cgaggagttc
     7621 ctgaaggcct tccacgaccg gctggcccat gtccatttgt cggataattt cggcaaaagc
     7681 gaggacctcc atcttcccct gcgggcagga accatcccct ggcctaagat catcggcttg
     7741 ttgaagaaat caggctatga cgggacgata accctggagg tcttctctca agagcggaaa
     7801 tatctgctcc taagccggga catcctccga cagctctggg gggagaaaaa cgcggtcagc
     7861 agtcaatgaa aagccatcca tcgacaaggc aaaagtgaga gataaagcca gcaaagtgga
     7921 gcaaactcga tgaaagctga tcgctgagat ctgatagctg catttttata gaaacaatgg
     7981 gatcatcgcc ggaaaagaaa atcattccct gcccttctcc agggtccccg ggagagatcg
     8041 aggactcatg gctcgccccg ccgaggatcg aagccatgga acccgtcgac ccggaaagag
     8101 cgggcagatc ggcgagcctt cccccccttt ccctcctcct cgtccattcc atggaccggg
     8161 aaaaggttga acgcatcaag gaagaagaaa aagtcccggt tatagtggtc ttcccggcga
     8221 gaggtgacga agagcttctg accctggagc gggaggtcca gatcctgcag ccccttatgg
     8281 gaagaattat cgacgaaatc tggatcgctt ttgggggaag cgaacacgac gacattcctc
     8341 tgatggccag aaagtatgga gccaaggtat tccatgccca tcgaaacggg atgaatgatg
     8401 cccgccgggg ggattcggga aagggacttt ccatgcgggg attactctac cacctcgtag
     8461 ctgatcgaca gctgaccgat ccccgggcca tcattcagtt catcgattcg gatatcaagg
     8521 aggggttctt caatccgcgc tgggtcatcg acccggcagg agcccttctc tggtttaaga
     8581 agatcgaagt agccaaaatc gtctaccacc ggcctttcgg gggaaggttg aacgccttca
     8641 ttaccccttt tctttccgta ttcgaccacc cggcgctgaa ccctctgaaa aacctgcttt
     8701 acttcctttc cggggaaatc gccgggacgc tgaagttctg gctttccgtt cccttcaagc
     8761 agaacttcgg aatcgaaatg aagatcctca cttccctggc cctaaacaaa gtaaggctgc
     8821 gtcagaaccg ggatgacctc agccatgtcc tccaggtctt catgggggag atggaccaca
     8881 agcacagcgc tctgcgcagc acggctcggg tcaaaggctt ggacgccatg gccaaggagg
     8941 ttttccagtc ctttctcgaa gaccttacgg cggaagggtt gatccgcatg gacaaggggc
     9001 tctgcttctc caaccgcctc caggtttcca cggttacccc cgccgccgaa ggaggggaag
     9061 aaatgggaac ccccagcagg ctcaaccttc cctccaatga aaagaccttc ttccccctga
     9121 aagacctccc cgagatccag gcccggtgtc cctggctaaa agccggttaa agggcgatcg
     9181 cgccatcccc cctctaatcc cgatgatttc ccttctgtgc gtatagaatg atggacttgg
     9241 gaaagaagta gttgcacacg tgctccagaa agcgaagagg cttggaaaag agggaatagt
     9301 agaggagctt cttgtaaaag gaggggatcg gagcattctc cctgtggagg ccggagaggc
     9361 agcggagcat ccagtaggga gaatggaagg catgggcaaa gcccacggcg tatacgcgga
     9421 ggttgttttc ctccatatat ccagccagct ttcggggaat aaccttgcgc acatggccgc
     9481 cgggagtctg gaaatactcc tcggacagcc aatcataaag gtgttccgtc atccgggtgg
     9541 gaacggtgat ggcgatcttt cccccctcgc tcagaatgcg ggcgagcgtg gctaccccat
     9601 ggcggtcgtc atcgacgtgc tcgatgacct ccgcgcagat gatcttgtcg aaactgccgt
     9661 cggcaaaggg aaaccgaagg gcatccccgc gcaggagaag aacgcttcct ttggattcct
     9721 tctttttttc catctgcccc aggacgtatt tggccttaag caggctgtga tggctcaggt
     9781 ccattcctag gatgcggcag tcatgccgaa agcactcaaa ggtgtggcgg ccttcgccac
     9841 agccggcatc gaggaccagg tccccctttt ccaggggaaa ggttcgaaaa ttaaccgtga
     9901 gcatcgatgg cctcttggta cacttccacc atctgccggg ccgcattctt ccaggtgaag
     9961 agcttcagca ctcttttcct cgccgctttc cccgtcttcc gacgaaggtc cgggtctctc
    10021 aggaaacgaa gaatggcttc cgccaaggcc ttggcgtccc gaggcgggac gagaaccccg
    10081 gcgccgtcat tccccaccac ctccggcagc gctcccgccg aagaagccac gactggaagc
    10141 tcgcaggcca tggcctcggc ggcggggaag ccgaagccct catagagcga tgggacgacg
    10201 gccacctcgg cattgttgta atggcggaca aggtcgccgg gcccgatctt cccggtaaag
    10261 tggacccggt gggcaatcct gtatttctcc gtgaggaggg gaccgaaagc cctttgggga
    10321 gctcctccat caacgacggt gagggtgacc tcttctggaa gatagacgag ggactgaagc
    10381 aggtagagca ggcctttttt ccggtcttcg gaatttccca cgaagatgag gttgccggtc
    10441 tttttggtcc atcccggaag aggagcgaat tcctcgctgt ccaggccgtt atagactacc
    10501 cggatttttt ctggcggcac cccgaaggcg tcggtgattt ccctggccga atcgtgggag
    10561 acggtgatga tccgatccat ccgacggctg acccatccct gcatgaggag gggataatag
    10621 agcaccctct tgactttttg cttgagggtg gaagggtttt cgaaccacgt ggcccgatcg
    10681 atggagaggg gatggtggat ggtggccacc actggaacgc ccagggtctt ccacaggacc
    10741 tggccgtacc ccaggcactg gttgtcgtgg agaatatgga aacggtgatg ccgcaccagt
    10801 tcccgcagtt tgaaataggc gcggaagctg aaggccttga tctccggaaa aaccccgaag
    10861 cgggaggaag cgaactcgta gaaattcagg ggagagaaaa tcttgaaagg atttccagag
    10921 ggtagaaaat ctgttttctt tccgaaaaaa ttgaaattct cgatggggtg gacaaatgac
    10981 ccgttcagaa aaagggggaa tggcggcccg acaatgacgt ggacctcatg gcccagtttc
    11041 accagctcgt tggccaggta agtgaggtaa atgccctgtc ctccgcagta cggattccct
    11101 cgataactca agaggcctat tttcacgacg atctccccga ataggcatag ggcagagggc
    11161 atagagcata gagtgttgcg ttaaaataac cgctcatctt cttttttcca tgcgctatga
    11221 tccatgcaat ctgcttattt aaagcagttc tttgtaaacc tccaccgtcc gttccgccac
    11281 cttcctccag gaaaagagat tctccaccct ttcccgaccc gccttcgcca tccgttccct
    11341 ccggcccctg tcttcaagca cttctcggat agccatggcc aaagctttgg catcccgggg
    11401 cggaaccaga atcccggccc cgtcttctcc gacgacctcc ggcagggctc cggcagaagt
    11461 aaccaccacc ggtgttccgc aggccatgga ttcagccgcg ggcaagccga aaccctcgta
    11521 aagagaagga actaccgtga cctgagcaga atggtattcc ctgcgcaggt tttcggggga
    11581 gagctttccg gtgaacgtta cccggcggaa gattcccaat ttctgaacga ggtccggggc
    11641 ataggtcttg gcgggacccc cttcatccac aatggtgaga ttcaccttct cgggcaggag
    11701 ggtcagagcc tggagcaaat accgggctcc tttcttgggg tcatccgtat tgccgacgaa
    11761 aaggacccgt tgggaccccc tcggagttcc gttccccggc cggaagaatt ccgtatccaa
    11821 tccgttggcc acattgcgaa ttcggtcccg ggagacccga aaggcttcct cgatttgcct
    11881 agccgtctcg cgggaagagg taaggacccg gtcacagcgg cggatcaccc tcccctgcat
    11941 tcctagggga tagaagacga cggtatagta tttttccttg aagttccgat cccgctccag
    12001 gctatgctga aagtcgatag tgagggggtg gtgcaccgta gtgaccagag gacggcgaaa
    12061 ccgcttcacc aggagaagcc cgtagcccag ggattgaaca tcatgaaaga cctggaaccc
    12121 ttcccgggga aagattttct ccaaggcccg gagcgcccgg acgctgaaga tgagcatttc
    12181 cggaaagaac ccgaaacggg tggcggcgaa ctcgaaaaaa ttaagagggc ggaagatccg
    12241 ccagggcgac acctggggga gaaaattctt tcttaccccc cagaggttca agctctccac
    12301 gggaagggtg cgggcccagg gcatgggcca gggatcggga ggccccacca gaacggtcgg
    12361 agaatgaccc atccgggcca gctcccggga gagaaaatag aggtaaaccc cttgcccacc
    12421 gcagtaagga ttgccgcggt aggagaggag acagatgttc atcctcggcc cctagggaat
    12481 ccctttctcg ggttctgagg agccggtcgg cgacggcttt ttcgcctccc ccgatggagg
    12541 agaaggaagt tcggcagccg ggggagcggt cttgatggtg agaatggtcc atccgatcca
    12601 gaatcccatg gccagaactc ccaggcaccc gatcatcacc gggatggcca gggcccagta
    12661 gctctgggct gaaatccccc agaggaagac caccgctgca atgaccatgg ccacgcagaa
    12721 aaaaccaccc caaacccgag aggtatccgc catttcagga actcctttct tttgccggaa
    12781 gagggctgtg cggcctgccc actaggatca ggacgtcgct ccaatccggg acccagttgg
    12841 cgattttatc cagcccgcga agaagaatca aggccattgg ttgagggatc cgctggagaa
    12901 agctcgacca ccggggaaat ccccacagga gggaaactcc ccggacggat tccattcgca
    12961 gatatttatt ggccaagcgc aggatcgtgg gatagtcgaa tttaaaagtg tggtcccggg
    13021 gaacttccca gatgtgaggc ggggggatct cccggcgcca gaccc
//
LOCUS       DASPRR010000316         9554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1848223, whole genome shotgun sequence.
ACCESSION   DASPRR010000316 DASPRR010000000
VERSION     DASPRR010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848223"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VEL68_11820"
     CDS             <1..306
                     /locus_tag="VEL68_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36698.1"
                     /translation="PEGFAGNIERGNMPIAGNPKKLAQDVANGFQQFTQASLRPYTLE
                     DLKVLLFNLNFVLRELRGKQIPLEDIEGLKDKNNRIHRINQAINIIQSFAATKGLKV"
     gene            622..1059
                     /locus_tag="VEL68_11825"
     CDS             622..1059
                     /locus_tag="VEL68_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXZ36699.1"
                     /translation="MDKVIPRSFFQSFWDTDVDDVFESFFEPSLPEKFNWAPKVESYS
                     KNGTYVVKADLPGVDAKDIQVTLENGCLTIKGERKVDKEVKEKKVERREFFYGSFERS
                     IRVPEGLKIEGVKAKYRDGVLEVTVPVEEKNPPKKIEVEEVKH"
     gene            1544..2812
                     /locus_tag="VEL68_11830"
     CDS             1544..2812
                     /locus_tag="VEL68_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ36700.1"
                     /translation="MAYWFENPTLKALAPLALSTSIKGLTTIINIPTLFIGSNVFPEG
                     PVVGPSTMDTLGNRCAKKRAFIVTDEFSKKFATKAARFLQAGGFSVETWPKCLPEAPM
                     EVVAECAKAMKAYEPDLIMALGGGSVMDSAKAAWVLYERPDITELGMISPLDKLNLRK
                     KALLAAVPTTSGTGSECTGAAVVHDTAAHRKIPLANVELVPDFAILVPEFTFSMPPKL
                     TAGTGLDVLAHAMDAVTTPAANEITEPLALKAIEMTFQWLPRAYKNGQDREARHRMIL
                     AASISGIAFGMSGCHLTHSFGHSLGAVFNLHHGMCVGFFLPQTLQFCSQVTDKHLLIC
                     ETLGIKAKDPKDGLVKLVARVRSFLTELEVPLTLKELGISRADFDAQFDKLVKFAFED
                     VDCYLSPRPITAAQCAQVFRYAYDGRDIDF"
     gene            2828..4657
                     /locus_tag="VEL68_11835"
     CDS             2828..4657
                     /locus_tag="VEL68_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ36701.1"
                     /translation="MKGYHGRLLEVDLSARKITDLPLSEEFLKKYIGGATLAAALVYE
                     RIGKNGSPLAAEKPLVFATGPFTGTSLPMVSRYAVGGVSPLTGFWGEATSGGRFPFRL
                     KGSGWDGLLVKGKAERPAFLYLKEGEAELRDASHLWGKDSYQTQKAIKDELKDDSASV
                     ACIASAGEKLIKFAGIMNDSGRAAGRCGLGALMGSKNLKAVAASGNLRPELANAEKVR
                     ELARQAVATINGNLVSVAFREYGTMMYMDMAMTLGDAPAKYFTKSIFPVSKITGQALR
                     QAYTVHNYACLGCPIGCGRELVEFKPGLNVDGPEYETTVAFGPLCMNTDLDSIVRANH
                     LCNVHGIDTISAGVSVAYAFYLYEKKVLKKDKVGFALKWGDGKAVVKLVEKIIAQKGI
                     GKILSQGTLAMAKEFKRSPDEAAQVKGLEMPMHDSRAFHGMAVSYATGPRGACHLKGD
                     YYNVDLGNMVLEYMILPGERLSSEGKSEGAAKYQSLKDLFDSLTLCKFAPLPPTQLCE
                     MLNAITGWQFSPKELLAAGDRSINIKRAVSNKLGLTREQDRMPRILTTALDEGTAAGV
                     EPDLEKMLKGYYQFRGWDWNTGRPTRERLLELGLNQVAAEMYP"
     gene            4713..4991
                     /locus_tag="VEL68_11840"
     CDS             4713..4991
                     /locus_tag="VEL68_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36702.1"
                     /translation="MKIRVQFHGVLSDWMGGAKAEVSLPEGGTFGELLMEIKKRFSPT
                     MPSQLRIKDQATFNRALWGMRGKDRLSELNAELKEGDEVQFFLSLAGG"
     gene            5231..6448
                     /locus_tag="VEL68_11845"
     CDS             5231..6448
                     /locus_tag="VEL68_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ36703.1"
                     /translation="MSRVPKRKVAIVGAFTTPCRGRWLSKTIWELAQWAVSGALQDAK
                     LKMDRVEAGVIGLYNDIFARQAIPESSFQGHMGLAFKPLVRVTNGGATGIYAFGTAFD
                     MVAGGRYDLVFCVGAEKATDCYDFMSETQTPEVVQTIAWSWDPLFERDQGATASDSYA
                     EVILAYMDQYPNDLKMPVRGEILRIMCEQAKNNPNAQRRDEIVTPERVANSPWIIEPA
                     FKKLETCVYTEGACCLIFAAEGVAEEICKDSGQPPIWIEGVGFANEPYWWGIKHPHKL
                     PGRIESDHLAAKAAYEMAGVGPKDIKVVELHDAFLPQLEITMAEMGFVPLGHADDLIE
                     KKIMAPHGRVLINPSGGLIYGGHFVGGSNMFSAWSARRELIRRGLDYALIHGTGASSA
                     QYGGAAVLKRGVI"
     gene            6448..7485
                     /locus_tag="VEL68_11850"
     CDS             6448..7485
                     /locus_tag="VEL68_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36704.1"
                     /translation="MEKLLRRPGDYVIPHEAIPEVMSRITVSPEDKFVCVERTEPMFT
                     TYWATAGEISPFFLAVMKEKKILGARCPKCKMVICPPYMTRCPVCQNDDYSLVNLETG
                     IEMPQVGYMLGTPPITVFANARFARYAPFGRGRVILGESQSALPIQVFTTTGFLKPGI
                     FKAGTKVKIVFRKFRMGFSTDYFAVPLDEVPEKLRDRNGLEETALKWKSMEVGEPQLT
                     DAFKKQFPKALQAVAKFVGAIPKSPRAQRDIANWTRKILVKTGGGKFGLLLDKQKIKV
                     MKDKVPRPDLVLVMEDPAHLIGWTNGDSLVNLIRTGGAGINNLQDMETIFKFDRLHRS
                     IRRDAEERGKK"
     gene            complement(7565..9544)
                     /locus_tag="VEL68_11855"
     CDS             complement(7565..9544)
                     /locus_tag="VEL68_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HXZ36705.1"
                     /translation="MSHPLLSTFQHPRLKKGKIVTAEEAVRVIRDGDTVATSGFVGAG
                     FAEEIAAKLEEYFLTVGKPRNLTLVYAAGQGDGSEKGLNHMGHEGLVRRVVGGHIGLA
                     PKLQKLIRENKILAYNFPQGVISHLFRDIAAHKVGTITTVGMGTYIDPRNDGGKLNEL
                     TKRGGEDLIKVIHLEGSDYLLYKAFPINVALIRGTTADTNGNITMEKEALTQEALAIA
                     MAARNSNGFVIVQVERIAENGTLNARDVKIPGILVDCVVLSRPENHWQTFATQYNPAF
                     SCEIKVPVQSIPPMEMSERKIISRRAAFELKANMVVNLGIGMPEGIAQVANEEKILDL
                     LTLTAEPGVVGGIPAGGLNFGAGTNMEALIDQPYQFDFYDGGGLDVAFLGLAQADQHG
                     NLNVSKFGPRFTGPGGFINISQRAKKIVFVGTFTAGKLKVAVEDGKLKIVQEGKERKF
                     LKLVEQITFSGKYAAETGQAVLYITERCVFQLTARGMELIEIAPGVDLERDILAQMDF
                     KPVIQKPRLMDARLFSSEPMGLKEELLSLPLEERLIYNHAENLFFVNFEGLYIKNREQ
                     VQKIKSLAEKTLAPLGRKVYTFVNYDNFNILPELVDEYTDMVKELVANYYEGVTRYTT
                     STFLRMKLGESLEKRNVAPHIYESREEARRALKKS"
BASE COUNT         2167 a         2575 c         2748 g         2064 t
ORIGIN      
        1 cctgaaggat ttgcagggaa catagaaaga gggaatatgc ccatcgctgg aaacccgaaa
       61 aagctagccc aggacgtggc caatggattt cagcagttca cccaagcctc tctccgtcca
      121 tataccctag aagacctgaa agtgctcctt ttcaacctca actttgtcct gcgggagctt
      181 cggggcaaac aaattcccct ggaggacatt gagggcttga aggacaaaaa caacagaatt
      241 caccgtatca accaggccat caatatcatc caatcttttg ccgccacgaa aggtctaaag
      301 gtctaaaact catccctccc tttgcccaac ccctttcctc tgggtctctt ctttactagc
      361 aaagccgtct taacggaaga aacttccctt gggaagagcc ggtttaaagc acttagggct
      421 cccactttat cgagaacctc caaaaagtca tgaatttcgg caaagcttgg gaaaagataa
      481 ttatttcaaa tatttagggc gagtctcttt cctaccttga cagtaggaaa tacagtgatt
      541 aaatttgaga cgtaaaagag aaatgagaag tgaagtccga agggaggtaa gaacatgaat
      601 agcttggtaa gatggagacc gatggataag gtaatccccc ggagcttttt ccaaagtttt
      661 tgggatacgg atgttgatga tgtttttgaa agcttctttg aaccttcttt gccggagaag
      721 ttcaattggg ctcccaaagt ggagagctac agcaaaaatg gaacctacgt ggtgaaagcg
      781 gacctcccgg gtgtagatgc caaggacatt caggtaaccc tggagaatgg atgtctcacc
      841 atcaagggcg aacggaaggt ggacaaagaa gtaaaagaga agaaagtgga gcgcagggaa
      901 tttttttacg gttccttcga gcggtctatt cgcgttccag agggcctgaa gatcgaaggg
      961 gtcaaagcca aataccgcga tggggttctg gaagtgaccg tcccggtgga agagaagaac
     1021 ccgcccaaga agatcgaagt ggaggaagtg aaacactaac cctgaaatcg aggaaaaatg
     1081 tcaaacaggc cgggtcaccc tggcctgttt ttttttaaaa caggatttct acagtccttc
     1141 gattttcact cagatgaccg accggctttt cgttaattct ctctcttttg cccggaaatc
     1201 aggcaaagat tcccctcccc tatcaagcct agcttccgca taaaattttc ttgacaaaac
     1261 ttttaacctt gctcatattg cccttgaagg atcggttcat tttttttgcc gaagcccgcg
     1321 ggaaatgacc atgggcccag agggtccagc cgagaagacc aacgggactt tctggagaaa
     1381 gggggtgata aattggcctt ttgaagtccc tgagggtctc tgcactcgca ttggaagctt
     1441 tcaaattcag agcaaaggga aacagggcgt ggtcgaaacg tctctggcgg agattgggat
     1501 ttccattgaa ggtttaactc tccaagaagg tgaggtgtga cccatggctt actggtttga
     1561 aaaccctacg ttgaaagccc tggcccccct ggccttgtcg acttccatca aaggtcttac
     1621 cacgatcatc aatatcccca ccttgttcat cgggtcaaat gtcttcccgg aaggaccggt
     1681 cgtgggaccc tctacaatgg acaccctggg caaccggtgc gccaagaaga gggcctttat
     1741 cgtaacggat gaattcagca aaaaatttgc gactaaggcc gcccgtttcc tccaggcggg
     1801 cggctttagc gtggaaacat ggcccaaatg cctgcccgag gcccccatgg aagtggtggc
     1861 cgaatgcgcg aaggccatga aggcatacga accagacctg attatggcct tgggcggcgg
     1921 gtcagtcatg gactcggcca aggcggcatg ggtcctgtac gagcggccgg acattaccga
     1981 gttgggcatg atttcccctc tggacaagtt gaatctacgg aagaaagccc tcctggctgc
     2041 cgttcctacc acttcgggaa cgggatcgga atgcacgggc gcggccgtgg ttcatgacac
     2101 cgccgcgcac cggaagattc ccctggccaa tgtagagctg gttccagatt ttgccatcct
     2161 ggtcccggaa ttcaccttct ccatgccccc caagctgact gctgggacgg gcctggatgt
     2221 cctggcccat gccatggatg cggtcacgac gccggccgcc aatgaaatca ccgaacctct
     2281 ggccctgaaa gccatcgaga tgacctttca gtggctcccc cgggcctata aaaacggaca
     2341 ggaccgggag gcgcgccacc gcatgatcct ggctgcgagc attagcggca tcgccttcgg
     2401 catgagcggc tgccatctga cccacagctt cggccattct cttggagcgg tcttcaacct
     2461 ccaccacggg atgtgcgtcg ggtttttcct tccccagacg cttcagttct gtagccaggt
     2521 gacggataag cacctcctga tctgcgaaac cctgggaatc aaggccaagg acccgaaaga
     2581 tgggttggtc aaattggtgg cccgggtccg ctccttcctg accgaactcg aagttccgct
     2641 caccttgaaa gaattgggga tttcgcgggc ggattttgac gcccaattcg ataaattggt
     2701 gaaattcgcc ttcgaggatg tggactgcta cctcagtccc cgccccataa cggccgccca
     2761 gtgcgcccaa gtcttccggt acgcctatga tggccgggat atagacttct aaagggaggg
     2821 tagaggcatg aaaggatacc atggccgtct gttggaagtg gatttatccg cccgcaagat
     2881 aaccgattta cctctatccg aggaattttt gaagaaatac atcggtgggg cgaccctggc
     2941 tgccgctctg gtttatgagc ggataggcaa gaacggcagt cctcttgccg cggagaaacc
     3001 cctggtcttt gccaccgggc ctttcaccgg gacttccctt cccatggtga gccggtatgc
     3061 tgtgggaggc gtttcgcccc tgaccggatt ctggggagag gcgaccagcg gggggagatt
     3121 tccctttcgc ctgaaggggt ccgggtggga cggcctgctg gtgaaaggga aggctgagcg
     3181 gccagctttt ctgtatttga aagagggaga agccgagctt cgggatgcct cccatctatg
     3241 gggaaaagac agttaccaga cccaaaaggc catcaaagac gaactgaaag atgactcggc
     3301 gagtgtcgcc tgcatcgcct ccgcgggaga gaagctgatc aaattcgccg gtatcatgaa
     3361 cgacagcgga cgggccgccg gaaggtgcgg ccttggcgcc cttatggggt cgaagaacct
     3421 gaaggccgtg gcggcttcgg gcaacctgcg gccggaactg gccaatgcgg agaaggtgag
     3481 ggagttagcc cggcaggcgg tggccaccat taacgggaac ctggtctccg tcgcctttcg
     3541 ggaatacgga accatgatgt acatggacat ggccatgact ctgggagatg cgccggccaa
     3601 atacttcacc aagtccatct tccccgtttc caaaatcaca ggccaggccc tgcgccaggc
     3661 atacacggtc cacaactacg cctgcctggg ctgccccatc ggctgtgggc gggagctggt
     3721 ggagttcaaa ccggggctca acgtggatgg gccggagtac gagaccaccg tggccttcgg
     3781 ccctctgtgc atgaacaccg acctggattc catcgtgcgg gccaaccatc tctgcaatgt
     3841 ccatgggatc gatacgatct cggccggggt gagcgtggcc tacgctttct acctttatga
     3901 gaaaaaagtt ttgaagaaag ataaggtagg gttcgcactg aagtggggcg acgggaaagc
     3961 agtggtcaaa ctggtggaga agatcatcgc ccagaagggc atcggtaaga ttctttccca
     4021 gggaaccctg gccatggcca aggagttcaa gcgcagcccc gatgaagccg ctcaggtcaa
     4081 ggggctggaa atgcccatgc atgactcccg ggccttccac ggcatggctg tgtcctatgc
     4141 caccggtccc cgtggggcgt gccatctcaa aggggactac tacaatgtag acctgggaaa
     4201 catggtcctg gagtacatga tcctgccggg ggagaggctc tcatccgagg gaaagagcga
     4261 aggagcggcc aagtaccaaa gcctgaagga cctctttgac tccctgaccc tctgcaaatt
     4321 cgcccccctg cccccgaccc agctctgcga aatgctgaac gccattacag gctggcagtt
     4381 tagccccaaa gagctgttgg ccgcggggga ccgatcgatc aacatcaagc gggcggtgag
     4441 caacaagttg ggcttgaccc gggaacagga ccggatgccc cggatcctaa cgactgccct
     4501 ggatgaagga accgcagccg gggtcgagcc ggatttggag aaaatgttaa agggctacta
     4561 tcagttccgc ggatgggatt ggaatacggg gaggccaacc cgtgagaggc ttttggaact
     4621 gggactcaac caggtggccg cggagatgta tccatagggg acgattgcgg attgtgggtt
     4681 tcggttgaaa gaaaaatgaa acctgatggc ctatgaaaat aagggtccaa tttcacggcg
     4741 tcttgtccga ctggatggga ggggccaagg cggaagtctc attgcccgag ggtggcacct
     4801 tcggtgaact tcttatggag atcaaaaaaa ggttcagccc caccatgcct tcccagctcc
     4861 ggatcaagga ccaggcaacc ttcaaccggg ctctatgggg tatgagggga aaggatcgat
     4921 taagcgaact gaatgccgag ctgaaggagg gagacgaagt tcagtttttc ctgtccctgg
     4981 caggaggata aaaattgtgg gggctgcccg atgtggccgt gcgcacgatg ggcagccaag
     5041 cggggttgcc cctacaattt cacctagcct ggatgcgttt ggtaaagaga atcttttttg
     5101 gcatttccac ggtgcgctat gatctatgct tcatgcgaat tcacgttcat tcggaagggg
     5161 ggatgaaaag ggttattgac gggttgagag ggtctgaatc ggcaatgaaa accataaaaa
     5221 agggggtttt atgagcagag ttccaaagag aaaagttgca attgtgggag cgtttaccac
     5281 cccctgtcgt gggcgctggt tatccaaaac catctgggaa cttgcccagt gggcggtctc
     5341 cggcgcgctg caggatgcca agttgaaaat ggaccgggtg gaagcggggg tcatcgggct
     5401 ttacaacgac attttcgctc gccaggccat tcccgaaagc tccttccagg ggcacatggg
     5461 attggctttc aaacctcttg tgcgggtcac caacggcgga gccaccggga tttacgcctt
     5521 cggcaccgcc tttgacatgg tggccggcgg acggtatgac cttgtgttct gcgtgggcgc
     5581 ggagaaagcc acggactgct acgacttcat gagcgaaacc cagacgccgg aagtggtcca
     5641 gaccattgcc tggtcctggg accctctctt cgagagggat caaggggcga ctgcttccga
     5701 ctcctatgcc gaggtcatcc ttgcctacat ggatcagtat cccaatgacc tcaagatgcc
     5761 cgtgcggggc gaaatcctgc ggattatgtg cgagcaggcc aagaacaacc ccaacgccca
     5821 gcggcgggac gagattgtga ctcccgaacg ggtggccaat tcaccctgga ttattgagcc
     5881 ggccttcaag aaacttgaaa cctgcgtcta taccgaaggg gcctgctgcc tgatattcgc
     5941 cgccgaagga gtggcggagg aaatctgcaa agactcaggg cagccgccga tctggattga
     6001 gggagtgggc tttgccaacg aaccctactg gtgggggatt aaacatcccc acaaactgcc
     6061 gggaaggatc gagtcagacc atctggcggc caaggccgcc tatgagatgg ccggggtcgg
     6121 cccgaaagac atcaaagtgg ttgagcttca cgacgccttc ctgccccaat tggagatcac
     6181 catggccgaa atgggctttg tgcccctggg acatgccgat gacctcattg agaaaaaaat
     6241 catggccccc cacgggaggg ttttgatcaa tccatccggc ggactcatct acggcgggca
     6301 tttcgtcggt gggagcaata tgttctccgc ttggtctgcc cgacgggagc tgatccggag
     6361 agggctggat tatgccctga tccatggaac cggagcgtcc tcggcccagt atggcggtgc
     6421 ggccgttctg aaaagggggg tgatctaatg gaaaagttat tgagaagacc cggggattat
     6481 gtgatccccc acgaagcgat tcccgaagtg atgagccgga tcacggtgtc tccggaagac
     6541 aaattcgtct gcgtcgagcg gaccgagccc atgttcacca cctactgggc cactgcgggc
     6601 gaaatatccc ccttcttcct ggcggtgatg aaggagaaga agatcctcgg cgcccgctgc
     6661 cccaagtgca agatggtcat ctgcccgccc tacatgaccc gctgcccggt ctgccagaat
     6721 gacgattaca gcctggtgaa cctggaaacg gggatcgaga tgccccaggt cggatacatg
     6781 ctggggacgc ccccgattac cgtctttgcc aatgccaggt tcgcccgcta tgcccccttc
     6841 ggccgtggcc gcgtgatcct gggagagtcc cagtcggccc tgcccatcca ggtctttacc
     6901 accacggggt tcctgaagcc gggaatcttc aaagcgggga cgaaggtgaa gattgtcttc
     6961 cggaagttcc gcatggggtt ctccaccgat tactttgccg tccccctgga cgaggttccg
     7021 gagaagctgc gggacaggaa cggactggaa gaaacggccc tcaagtggaa gagcatggag
     7081 gtcggagaac cacagttgac ggatgccttc aagaagcagt tccccaaagc ccttcaggcg
     7141 gtcgccaaat ttgtcggcgc catccccaag agcccccgtg cccagcgcga cattgccaac
     7201 tggacccgga agattctggt caagaccggc ggcggtaaat tcggactgct cctggacaag
     7261 cagaagatca aggtgatgaa ggataaagta ccccggccgg acctggtcct ggtgatggaa
     7321 gacccggcgc acctcatcgg atggaccaac ggcgattccc tggtcaacct gatccgcact
     7381 gggggtgccg ggatcaacaa cctgcaggac atggaaacga tcttcaagtt cgaccgtctc
     7441 caccggtcga tccgccggga tgcggaggag aggggaaaga aataaaatcg taggggcgac
     7501 ccgccgggtt gcccctgcca tgttgactcg gtgtaggggc aggtttgaaa cccgcccctg
     7561 cgaattaact cttctttaag gcccttcttg cttcctcccg gctttcgtaa atgtggggag
     7621 ccacgttccg cttttccagg gattctccga gtttcatccg caggaaggtg ctcgtcgtgt
     7681 agcgggtcac tccctcataa taatttgcca ccagttcttt caccatgtcc gtgtactcat
     7741 ccactagctc gggcaaaatg ttgaagttgt cgtaattgac gaaagtgtag accttccgtc
     7801 ccaggggcgc cagggttttc tcagcgaggg atttgatctt ctgaacctgc tcccggtttt
     7861 tgatgtacag cccctcgaaa ttcacgaaaa agaggttttc ggcatggttg taaatcaatc
     7921 tctcttccag ggggagagaa agaagttctt ctttgaggcc catgggctcc gatgagaaaa
     7981 ggcgggcatc catcagacgg ggtttctgga tgaccggctt gaaatccatc tgggccaaaa
     8041 tgtccctttc aaggtcaacg ccgggggcga tttcgatcag ttccattccc cgggcagtca
     8101 gttggaaaac gcaacgctcc gtgatgtaaa gaacggcctg ccctgtttcc gcggcatatt
     8161 tcccgctgaa ggtgatctgc tccacgagct tcaggaattt tctctctttc ccttcttgga
     8221 cgatcttaag ctttccgtct tcgacagcga ctttcagctt tcctgcggtg aaagttccca
     8281 cgaatacaat tttcttggct ctctggctta tgttgataaa cccgcctgga ccggtgaagc
     8341 ggggcccgaa tttgctcacg tttaagtttc catgttgatc agcctgggcc aggcccagga
     8401 aagccacatc cagaccaccc ccgtcgtaga agtcgaactg gtaaggctgg tcgatgagcg
     8461 cttccatatt ggtgccggcg ccgaagttca ggccgccggc cgggattcct ccgaccaccc
     8521 caggctcggc ggtgagggtt aagaggtcca ggatcttctc ttcgttggcc acctgggcga
     8581 tcccctcggg catcccgatg cccaggttca ccaccatgtt ggctttaagc tcaaacgccg
     8641 ccctccggga aatgatcttt cgttcgctca tctccatggg ggggatagat tgcacgggga
     8701 ccttgatctc gcagctgaag gccggattgt actgggtggc aaatgtctgc cagtggttct
     8761 ccggccggct gaggaccacg caatccacca ggatccccgg gatttttaca tcccgggcat
     8821 ttagcgttcc gttttccgca atcctttcca cctgaacaat aacgaacccg ttggaatttc
     8881 tcgccgccat ggcaatggcc aaggcttcct gggtgagagc ctccttctcc atggtgatgt
     8941 tgccgttggt atccgccgtc gtgccccgga ttaaggccac attgatggga aaggctttgt
     9001 acaggagata gtccgatcct tccagatgaa taactttgat aaggtcttcc cctcctcttt
     9061 tggtcaactc gttcagcttt ccgccgtcat tccgcggatc gatgtaagtt cccatgccta
     9121 ctgtcgtgat ggtgcccacc ttatgggcgg cgatgtcgcg gaagagatgg gagatgaccc
     9181 cctgggggaa gttgtaggcc aggattttgt tttcccggat cagcttctgg agtttggggg
     9241 ccaggccaat gtgcccgccg acaaccctgc gaacaagccc ttcatggccc atgtgattga
     9301 ggcctttctc agacccgtcc ccttggccag cggcatacac cagggtcagg tttcgcggct
     9361 ttccaacagt caaaaaatat tcttccagtt tggcggcgat ttcctcggca aagccggccc
     9421 ccacaaagcc gctggtggcc actgtatcgc cgtcccggat gacccgaacc gcttcctcgg
     9481 cagtgacgat cttgcctttt ttcagccggg ggtgttggaa agtggataat agggggtgag
     9541 aaatcgcttt ttca
//
LOCUS       DASPRR010000317         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1197725, whole genome shotgun sequence.
ACCESSION   DASPRR010000317 DASPRR010000000
VERSION     DASPRR010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197725"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..2042)
                     /locus_tag="VEL68_11860"
     CDS             complement(402..2042)
                     /locus_tag="VEL68_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXZ36706.1"
                     /translation="MKSSFWSLTTVLIFIVFFFFMIIPVISLFTGSFQLEEGGQFSFE
                     NYTNFAKYPSYWRSFYHSIVIGLISAAGAILIGVPMGFMVARWNVMGKPLIITLSTLP
                     LMLPTFIAAFAWIILLGRGGIVTKYLEALGIQIGSIYGYPGMILVFVSQLYSYVFLMT
                     LSGFSSIDESLEEASRSLGASPLRTFIKVSFPLVLPSILSGALLTFMSAIENFGVPMV
                     IAQQTPNLVVKAYVEFTSEVGKHPGMAYTLSVVLIFITMASLIVQRYYLSRRNFIQAA
                     RGKPLVKQLTPGKRLAASAVIYAILIFTLIPFFVAFILSFMEMRGPVFYPKFSLQSYI
                     YAFERSARPIFNTYFLATVATIASVLIGVPTGYILTRRKSIYSNMLDIVIMLPFVIAG
                     TILGIALITFFNKGWLVLTGTWVILALSYVIRKMPFIARSGASILYQLDPSLDEASIS
                     LGVSPMKTFYKVTFRLMMAGIVSGSILSWVTIISELSSTIVLQTPGWSTMTVEMYQGI
                     ISDDLGKAAAFSSIMIVSTIIPLLFITKYFSKDQTSLL"
     gene            complement(2111..>2773)
                     /locus_tag="VEL68_11865"
     CDS             complement(2111..>2773)
                     /locus_tag="VEL68_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008531057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36707.1"
                     /translation="SGGQLQRVAIARALVIEPQVLLMDEPLSNLDAKLRIEMRSEIRE
                     LQRQMRITTVYVTHDQEEALSISDRIAVMNLGVVEQVGRPWEVYNTPTNKFVAGFIGT
                     TNFFEGQWTGPEGQVGVSGIKMKISGQPRSAGENVFFAIRPEAFKVAEQLTDEERKEM
                     FRLPGKVAKVEYLGYMTKYEMELGQDLNVKMVSYDVLPEDLRKEGEAVELFYDPKRVL
                     VY"
BASE COUNT          686 a          712 c          755 g          620 t
ORIGIN      
        1 aatatttggc tgcaagggcg aatattcagc agattccgat aattcctttg taaagttgcc
       61 tctcctgctt gccattacca tataataagt ccttcccaga aatcccccct acccccgtct
      121 ttgccaaagc ggggcgaaga acgcgggcac ccgccgggtg cccttacatc attacccaac
      181 ggcgtgaatc gaaaaaccaa caccgccgcg cgaattgcca tgtgcctatc tccaaggaaa
      241 taattacctt tcgaaatccc ctccagcccc ctttcattac atccgtatgc gcagagttat
      301 tcttgttcta accttaattc ttttctgcag gattgatatc tctgtcctcc atatgatctg
      361 cctaatctgt gaattctgcg gacaaatagt ttccgaaatt cctacaggag agaggtttgg
      421 tctttgctga aatatttggt tatgaacagt aagggaatga tggtggaaac aatcatgatt
      481 gacgagaagg cggcggcctt gcccaggtcg tcgctgatga tcccctggta catttccacg
      541 gtcatggtgg accatcctgg agtctggagg acgatggtag aacttaactc cgaaataatc
      601 gtgacccagg aaagaatgga cccggagacg attccggcca tcatcagccg gaaagtcacc
      661 ttatagaagg tcttcatagg cgagaccccc agggagatgc ttgcttcatc cagagagggg
      721 tcaagttgat agagaatcga agcgccggag cgggcgataa agggcatctt tcggatcaca
      781 taggagagtg cgagaatgac ccaggtcccg gtcaggacca gccacccctt gttgaagaaa
      841 gtgatcaggg cgatacccag gatcgtgccg gcgatcacaa agggcagcat gatcacgata
      901 tccagcatgt tgctgtagat cgattttcga cgggtgagaa tgtacccggt ggggacaccg
      961 atgaggaccg aggcgatggt cgccacggtg gccaggaaat aggtattgaa aattgggcgg
     1021 gcggagcgct caaaggcgta gatgtagctc tgcaggctga acttgggata gaaaaccggc
     1081 cccctcatct ccatgaagga gaggatgaag gccacgaaga aggggatcag ggtaaagatg
     1141 aggatggcgt aaataaccgc gctggccgca agtctctttc ccggcgtaag ttgtttgacc
     1201 aacggcttgc ccctggccgc ctggatgaag ttgcgcctgg aaagataata tcgctggacg
     1261 atcagagagg ccatggtaat gaagatgagg accacggaaa gggtgtaagc catacccgga
     1321 tgtttgccca cctcgctggt gaattcaaca taagctttga ccaccaggtt tggcgtctgc
     1381 tgggcaatta ccatggggac gccgaagttt tcgatggccg acatgaaagt caagagagcc
     1441 ccggaaagga tcgaagggag gaccagaggg aaggagactt ttatgaaggt ccgcagtgga
     1501 ctggctccca ggctccggct ggcctcttcc agggattcat cgatggagct gaaacctgac
     1561 aaggtcatca gaaagacgta tgagtagagc tgggacacaa agaccagaat catccctgga
     1621 tatccgtaga tggagccgat ttgaattccc agggcctcca agtacttggt tacgattccc
     1681 ccccgtccca agagaatgat ccacgcgaaa gcggcgatga aagtgggcag catgagggga
     1741 agagtggaca gggttatgat gagaggtttg cccatcacat tccatcttgc caccatgaag
     1801 cccatgggaa ccccgatcag gatggcccct gcggcggaaa tgagcccgat gacgatggag
     1861 tggtagaagc ttctccaata ggaagggtat ttcgcaaaat tggtatagtt ttcaaaggaa
     1921 aactgacccc cttcttccag ctgaaaactt cccgtgaaaa gactgatcac cgggatgatc
     1981 atgaagaaga aaaagacgat gaagatcagg actgtggtta agctccagaa agaggatttc
     2041 atggaaagct caatttatct tcgccccctc ccgtccctcc cttctccgag gggagggcaa
     2101 tggcggggga ttaataaacc aggacccttt tcgggtcgta aaagagttct accgcctccc
     2161 cttctttacg caggtcctcc gggaggacgt cgtaggagac cattttgacg ttcaggtcct
     2221 gtcccaactc catctcgtac ttggtcatgt acccaaggta ctccactttg gcgaccttgc
     2281 ccggcaggcg gaacatctcc tttcgttcct catccgtaag ctgttcagcc accttgaagg
     2341 cttctgggcg aatggcaaag aagacatttt ctccggccga acggggctgg cccgaaattt
     2401 tcatcttgat tccgctgacc cccacttgcc cttccgggcc ggtccattga ccctcgaaga
     2461 agttggtggt cccgatgaat ccggccacga atttgttggt gggggtgttg taaacctccc
     2521 aaggccttcc cacctgctcc accaccccta ggttcatgac tgcaatccgg tccgagatgg
     2581 aaagtgcctc ttcctggtcg tgggtgacat aaacggtagt gatccgcatt tgcctctgta
     2641 gttcccttat ctcgctgcgc atctcaattc ttaacttggc gtccaggttg ctcagcggtt
     2701 cgtccatgag taatacctga ggttcaatca ccagggcccg ggcgatggcc actcgctgga
     2761 gctggccgcc gga
//
LOCUS       DASPRR010000318         7634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1038407, whole genome shotgun sequence.
ACCESSION   DASPRR010000318 DASPRR010000000
VERSION     DASPRR010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038407"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..148
                     /locus_tag="VEL68_11870"
                     /pseudo
     CDS             1..148
                     /locus_tag="VEL68_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097389.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
     gene            375..908
                     /locus_tag="VEL68_11875"
     CDS             375..908
                     /locus_tag="VEL68_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ36708.1"
                     /translation="MKVIGVHLDRCTGCKTCELYCAAERGSDGKTLLRAVQESPTPKP
                     RVRVEGNNQLPIALQCRHCLNAPCVDACLTGALTREPKSNLVVVQEDRCIACWTCTMF
                     CPYGVIYPWPDRKIALKCDRCAFMDNPVCVEVCPCKALELVDVEEFDKIFENRRQEVS
                     KRIAAKKTRGMLLLDLK"
     gene            1014..2483
                     /locus_tag="VEL68_11880"
     CDS             1014..2483
                     /locus_tag="VEL68_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36709.1"
                     /translation="MESIQVKTVDPISQELLKSAYQRGLKLSWDRFEKLQPQDGFLRI
                     GLSCPYGCLQGPCRIDPFGRGSDRGLCGLDREGMAAALLLRLTLSGALEVMPEDKPTS
                     EPSWAPSLKEALPRALKNLAKDRVSPVEVQKAANLLNRPMESAEGMIIQALRLALLTI
                     GWLSKGPASQEGKKTLPFRVGYGLLAQKEINIGICGRPSPEFLKALLAEVDKNLSSIG
                     QVLSLGEFISLDGICLPSVCTSGEAELVLSSGKINLLIAGPGTDPSILEVCRTLNIPS
                     PSSMDPKSIRDLVAKAKVTQSVSILTGFNPQPSLVEEAEVIAASSILEESFKKSPAKK
                     LALLGGADHPYHALGWIPTEVAPALLGKGFAVTGWGDCGIWMVKKGLASGRNKRPVQI
                     LDGRQGPVLALKALAASGRLKDVKGIFYTGVKSCQDLAVALGLAVLGLRVGMAVPLPL
                     WGSEKVRNLLQEKLAAMGGSLTHFDHPAQAEEILDWFIK"
     gene            2573..3841
                     /locus_tag="VEL68_11885"
     CDS             2573..3841
                     /locus_tag="VEL68_11885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HXZ36710.1"
                     /translation="MDIVIVGSGPAGIQAAIQSRQSWPRKSVTLVEGESHVGYCRPLL
                     PQFMAGQAKEEKLFLFKPGEDPLLQVKTGVKVQSLERKRRVLHLENGEEIRYERLILA
                     PGGRPIIPRLEGVEGLEGIFSVRNLPEAQKARSWITQEQKIIVLGGGLVGVKTAAYLR
                     SAGFQVSVVEKEDRLLPQALKADSAGYVENHFRQMGIGLYLGRTLEAAEGEDGKIKRA
                     RVGEQWVPCDTLLLAIGSIPNISFLGSSGLLEDDKLRVSAALQTKDPGIFAAGDAVTI
                     FAADGKKYTPWTWPQAVSQGKLAAANLYRSQPIPLKALTRVNSMNLQGLSLNMLGPFE
                     EGSEEVSYAPQGGEIRRQVFLHDGRIIGGALVGDITGAGPLHHLMISGKDSAQEILRL
                     IKPSFGLMPRGLSDRGLERRRARLIPGEGK"
     gene            3844..4581
                     /locus_tag="VEL68_11890"
     CDS             3844..4581
                     /locus_tag="VEL68_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ36711.1"
                     /translation="MLIRKDLCVGCGRCQPYCPVEAIRFEELKSIVDQEVCYECGTCL
                     RSEVCPVEAIEESPYVYEYPRALRKFFSDPNATHALTGIAGRGTEESKSNDVINRVVP
                     GEVGMAIEFGRPTLGMDLTNIQKVTRAMAKAGFTKIEPENPFNDMIQNYETGDLKPEL
                     MGEKVLSAIMEFKITPDRLGLALRTIKEAAKEVDSVFVVDVYTRVGPGLTIPREVLDP
                     IEAEGYTCRPNAKINMGLGRANKRKDA"
     gene            4578..5150
                     /locus_tag="VEL68_11895"
     CDS             4578..5150
                     /locus_tag="VEL68_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36712.1"
                     /translation="MTHSLHREGPLGLLEKEFVLFVYPARGFNYDGSGPKVRRLGEML
                     FHQGPANLLVTTLRQNMYLAVSQTEILNSIKDGAKIFCVLENREKIKDTLAKIKEMDE
                     GISIVISGVIDRVREISAELGIDPHTVNLSLGTHGRKDRLPPADIRQFTTMCGHGMVS
                     PNLIRDLIRRVKTGKLDLWGASLLAAAPCA"
     gene            5453..6178
                     /locus_tag="VEL68_11900"
     CDS             5453..6178
                     /locus_tag="VEL68_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ36713.1"
                     /translation="MRIDAEKCIGCETCIPFCNVGAISMQDDVAVVDDKVCVECWVCY
                     RNRVCPQDCFEPTPLKSPGDIFKHVLSDPTVTSEGTGVPGRGTEEAKTNDVTGRFTTD
                     TYGICIDMGRPGVGTYMRDVEKVAMAAAGAGVRFASPKETPLSGLMTDIRTGKIRDEY
                     LPVHVLSVIIEGTCKREKLGPVLEALVKVEKEIETVFSLGIVSMAVGDGDEPLFRTLQ
                     QHGLEKPKRGKVNVGLGKPLFRG"
     gene            6295..6918
                     /locus_tag="VEL68_11905"
     CDS             6295..6918
                     /locus_tag="VEL68_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36714.1"
                     /translation="MTHSLHRCGKLQEKDFVWLLYHVKGVNDQNLVERLRKAIAIIEE
                     VGSVNWGDVKSGPIVSVSAAEIKEKLTEKSRIRGAFTSRNQVTNFLKKMKEADLGFCV
                     IISGLLDQVFASCREAGVKPHTMNYSIGVFGKKDLLADDDTLAITTMCGHHMIPDEFV
                     TEARKRVKEGKLSPEKASQEFAELCPCGIFNQERAKELLILEKENPA"
     gene            7444..>7634
                     /locus_tag="VEL68_11910"
     CDS             7444..>7634
                     /locus_tag="VEL68_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36715.1"
                     /translation="MKKLIWVGFLGLLILGTSAGSVLAQGKYPTKAIDIIVPYAPGGG
                     TDIMYRNIEKIISQYKLVS"
BASE COUNT         1788 a         2031 c         2201 g         1614 t
ORIGIN      
        1 ccgtggccgg gggtcatccc atcgtgatta ccacccccgc gcacgggacg gcccacgata
       61 tcgccggaag ggtatcgccg acatcggggc cttccaggcg gccttgcgaa tcgcagtacg
      121 catggccctg cattccaaga aaagctgacg atgatttagc ttgctttgtc ccaaaggggc
      181 atttagaatt tatttataag cagtcagtat caaatccgaa gcacgaaatt cgaaatccga
      241 aacaaattcg aatttcataa atcccaaaat tccaaacggg atcacacagg ggcgccctgt
      301 tttgagattc ggatttaatt attgtttcgg aattcgaaat tcgagtttcg gattttttct
      361 gtgtgggggt tttcatgaag gtcatcggag ttcatctgga tcgttgcacc ggatgcaaaa
      421 cctgcgaact gtactgcgcg gctgaaaggg gaagcgacgg gaaaaccctc ttaagagccg
      481 ttcaggagtc cccgactccc aagcctcgcg tccgcgtgga aggaaacaat cagttgccca
      541 tcgccctgca gtgccgccac tgcctcaacg ccccctgcgt cgacgcttgc ctgacaggag
      601 ctttgacccg ggagcccaaa tccaacttgg tggttgttca ggaggaccgc tgtatcgcct
      661 gctggacctg cacgatgttc tgcccctacg gagtgatata tccctggccg gaccggaaga
      721 tcgccctgaa atgcgaccgg tgcgccttca tggacaatcc ggtttgcgtg gaggtctgcc
      781 cctgcaaggc gctggaactg gtggatgtgg aagaattcga taaaatattc gaaaaccgta
      841 ggcaggaagt gagcaagaga atcgccgcaa agaaaacccg tggaatgctt ttgcttgatt
      901 tgaaataatt ttcatctttc agccttatag gggcgggttt aaaacccgcc cctactccct
      961 ggacgaagaa aaaccccgat tttcattttg cgatatgaag ttggaggata ttcatggaat
     1021 cgattcaggt caagaccgtt gacccgatca gtcaagagct tttaaaatcc gcttaccaga
     1081 gggggctcaa gctgagctgg gaccgctttg aaaagcttca gccccaggat ggtttcttgc
     1141 ggatcgggct gagctgcccc tacggctgcc tccagggccc ctgccgcatc gaccctttcg
     1201 gccgcggatc ggaccgcgga ctttgcggtt tggaccggga agggatggcc gcggcactgc
     1261 tgcttcgcct aaccctcagc ggagccttgg aagtcatgcc ggaagacaag ccgacttccg
     1321 aaccctcctg ggctccctca ttgaaagaag cgctccctcg agcgttaaag aacctcgcaa
     1381 aggaccgggt ctcccccgtg gaggtccaaa aggcggccaa cctcttgaac cgccccatgg
     1441 aatccgcgga aggtatgatc atccaggctc tgcggctggc ccttctgacc atcggctggc
     1501 tttcgaaggg accggcatcc caggaaggga agaaaacgct ccccttcagg gtcggctatg
     1561 gccttcttgc ccaaaaggag atcaacatcg gaatctgcgg ccggccttca ccggaattcc
     1621 tgaaagcgct cctggccgaa gtcgacaaaa acctttcctc catcggacag gttctctccc
     1681 tgggcgaatt catttccctg gatggaattt gcctgcccag cgtctgcacc tcgggtgagg
     1741 ccgagttggt attgagctcg ggaaaaatca acctcttgat tgccggaccg ggcacggacc
     1801 cttctatcct cgaagtctgc cggactctga atatcccttc accttcctcc atggatccca
     1861 aatcaatccg tgacctggtt gcaaaagcca aagtgaccca aagcgtttcc attctaaccg
     1921 gattcaaccc tcagccgtct ctggtggaag aagcggaagt tatcgccgcc tcttcgattc
     1981 tcgaagaatc cttcaaaaaa agtcctgcta aaaagctggc tcttctgggg ggggctgatc
     2041 atccttacca tgccctgggt tggattccga cggaagtagc tcccgcactt ctaggcaagg
     2101 gttttgcagt caccggctgg ggtgactgcg gaatatggat ggttaagaag ggcctcgctt
     2161 ccggcagaaa taaaaggccc gttcaaatcc tggatgggag gcaggggccg gttttagcat
     2221 tgaaagccct ggccgcctcc ggaaggctga aggacgtcaa ggggattttc tacacgggag
     2281 ttaaaagctg ccaagacctg gcggtggctc tgggcttggc tgtcctgggt ctcagggtcg
     2341 gcatggctgt ccccctcccc ctgtggggaa gcgagaaagt ccggaatctc ctccaggaaa
     2401 agctggccgc catgggagga agtttaaccc acttcgatca cccggcccag gccgaagaga
     2461 ttctggattg gtttataaaa taaccgtttc gagtttcggg ttccgggttt caggtttttc
     2521 cgttaactcg aaacttgaaa ctcgaaagcc gaaacctaat tagagatatt ttatggacat
     2581 cgtaatcgtt ggcagcgggc ccgcgggcat tcaggcggct attcagagtc ggcagagctg
     2641 gccccgcaag tccgtaaccc tggtcgaagg ggagagtcac gtcgggtact gtcgacctct
     2701 ccttcctcaa ttcatggccg ggcaggccaa agaggagaag ctctttctct ttaaaccagg
     2761 ggaagatcct ttgcttcaag tgaaaaccgg ggtgaaggtt caatccctgg aaaggaaaag
     2821 gcgggttctt catctggaaa acggagaaga aatcagatac gaacggctga tattagctcc
     2881 cggcggccgg cccatcatcc cccgcctgga gggagtcgag ggcctggagg gaattttctc
     2941 cgtccgcaat ctccctgaag cccaaaaggc gcgaagctgg ataacccagg agcaaaaaat
     3001 cattgtcctg ggcggcggat tggtgggagt caagacggcg gcttatctgc gatcggcggg
     3061 ctttcaagtc tcggtggtgg aaaaggagga ccgtctcctc cctcaggcat taaaagctga
     3121 ttcagccggg tacgtggaaa atcatttccg tcagatgggg attggccttt atttgggccg
     3181 gaccctggag gccgctgaag gggaagatgg aaaaatcaag agggccagag tgggagaaca
     3241 atgggtcccc tgcgataccc tgcttctggc gatcggttca atcccgaata tttctttcct
     3301 cgggagttcc gggttgctgg aggatgataa gcttcgggtt tcggccgctc tgcagaccaa
     3361 agaccccggg atttttgctg ccggcgacgc ggtcacgata tttgctgcgg acgggaagaa
     3421 atacactccc tggacttggc ctcaggccgt ttcccagggt aaacttgccg cggcgaatct
     3481 gtatcgctct caacctattc ctctcaaagc cttaacccgg gtgaactcca tgaaccttca
     3541 aggcctctct ctcaacatgc tgggaccttt cgaggaaggc tccgaagagg tttcttatgc
     3601 tccgcaaggc ggggaaatca ggcgccaggt cttcctgcat gatggccgaa tcattggagg
     3661 ggccctggta ggagatatca ccggcgcagg gcccctgcat cacctgatga tcagcggaaa
     3721 agactctgct caagaaattt taagactcat aaagccgtcc ttcggcctca tgccccgggg
     3781 tctgtcagac cgcggcctgg agcgcagaag ggccaggctc attcctggag aagggaaata
     3841 gctatgctga tccgaaaaga cctctgcgtc ggctgtggac ggtgccagcc ctactgcccg
     3901 gtagaggcca tccgcttcga ggaactgaag agcattgtgg accaggaggt ctgttatgaa
     3961 tgcggaacct gcctgcggtc cgaggtctgc ccggtggagg ccatcgagga atcgccgtac
     4021 gtgtatgagt atccccgggc gctgcggaaa ttcttcagcg acccgaatgc cacccatgcc
     4081 ctgaccggca tcgcggggcg gggaaccgag gagtcgaaga gcaatgatgt catcaaccgg
     4141 gtcgtaccgg gagaagtggg gatggccatt gaatttggaa ggcccaccct gggaatggac
     4201 ctgaccaaca ttcagaaggt cacccgggcc atggccaagg ccggcttcac caagatcgag
     4261 cccgaaaacc ctttcaatga catgatccaa aactatgaaa ccggcgacct gaagccggag
     4321 ctcatggggg agaaggtttt aagtgcgatc atggaattca agatcacgcc cgatcgactc
     4381 gggctggctc ttcgcaccat caaagaagcg gccaaagagg tggacagcgt cttcgtggtg
     4441 gacgtataca cccgggtcgg gccggggctc accatcccgc gggaagtgct tgatccgatc
     4501 gaggccgaag gatatacctg tcgtccgaat gccaagatca acatgggcct gggaagggcc
     4561 aataagagga aagacgcatg actcacagct tacaccgtga aggaccctta ggtctgctgg
     4621 aaaaggagtt tgtcctcttc gtctacccgg caaggggatt taactatgac ggcagcggac
     4681 ccaaggtcag gcgccttggg gagatgctct tccaccaggg tccggccaac ctccttgtta
     4741 ccaccctccg gcagaacatg tatctcgcgg tttctcaaac ggaaatcctg aacagtatca
     4801 aggatggggc caagattttc tgcgtgctgg aaaatcggga aaagatcaaa gacaccctgg
     4861 ccaaaatcaa ggagatggac gaggggatct cgatcgtgat cagcggggtc atcgaccggg
     4921 tgcgggagat ttcggcggaa ctcggcatag atccccatac cgtcaatctt tccctgggaa
     4981 cccacggccg gaaggaccgc ctcccgcccg cggacatccg ccagttcacc accatgtgcg
     5041 gccatggcat ggtgtctccg aacctgatcc gggacttgat ccgaagagtc aaaacgggga
     5101 agctggacct ctggggggca agcctgctgg ccgccgcccc ctgcgcctga gggatcttca
     5161 acccctgcag gtctgcagag atgcttaagg aactggttcc cctctatacc gtgaatcgtc
     5221 tttgagtgtg gcaggtatca aaagacaagc gaaaaagtcc aaatcctgca gcctcttcat
     5281 gaggacctga cccggccacg ccggaagcaa aaaggatatt gtaggggcgg ccccccgtgg
     5341 ccgcccgcaa aaagggcaac cacggggggt tgcccctaca gtttgcagcc atcttggacg
     5401 caattttact tttaagggct aagaatgctg tggattttta ttggaggaga agatgcggat
     5461 tgatgccgag aaatgtattg gatgcgagac ttgtatcccc ttttgcaacg tgggtgccat
     5521 ctctatgcaa gatgacgtgg cggtcgtcga cgacaaggta tgcgtggagt gctgggtctg
     5581 ttaccgcaac cgggtatgcc cgcaggactg ttttgaaccg acgcccctca aatccccggg
     5641 ggacattttc aagcatgtcc tgagcgaccc cacggtcacc agcgagggga cgggggtccc
     5701 gggacggggt accgaggaag ccaagaccaa cgatgtgacc ggaagattca ccacggatac
     5761 ctacggcatt tgcatcgata tggggaggcc cggagtgggc acctacatgc gggatgtgga
     5821 aaaagttgcc atggcagcgg ctggagccgg ggtccggttt gccagcccga aggaaacccc
     5881 gctttcgggt ttgatgaccg acatccggac cggaaaaatc cgcgatgaat atcttccggt
     5941 tcacgtctta tccgtgatta ttgaaggaac ctgcaaaaga gaaaaactgg gccccgttct
     6001 ggaagccctg gtgaaggtgg aaaaggagat cgagaccgta ttcagcctgg gaatcgtttc
     6061 catggcggtc ggcgacgggg atgaaccgct tttcaggact ctgcagcagc atgggttgga
     6121 aaagccgaag cgcgggaagg tgaatgtggg gctcggaaag cctcttttta gaggctgaga
     6181 ccccgcgctt tttaagcggt ccctggaaaa gaacttgcag gggcatcccg cctttcgcgg
     6241 gaggttgagc ctctacgaat gctcgtccta caaccctggg caggagaaga taccatgact
     6301 cacagtttgc accgttgcgg gaaattgcag gagaaggact ttgtctggct cctgtatcac
     6361 gtgaaagggg ttaatgacca gaatctggtg gaaaggctgc gcaaggccat cgccatcatc
     6421 gaagaagttg gatcggtaaa ctggggggac gtgaagtccg gccccatcgt gagcgtttcc
     6481 gcggcggaaa tcaaggaaaa gctcaccgag aagtcgagaa tccggggggc tttcacttcc
     6541 cggaaccagg tgacgaattt cctgaagaag atgaaggaag ccgatctcgg gttctgcgtg
     6601 attatttccg ggttgttgga tcaggttttc gcttcctgcc gggaagcggg agtgaagccc
     6661 catacgatga actattctat cggggttttc ggcaaaaaag accttctggc cgatgacgac
     6721 accctggcca tcaccaccat gtgcggccat cacatgatcc ccgatgagtt cgtgaccgag
     6781 gcccggaaga gagtgaaaga gggaaagctg tctcccgaga aagcgagcca ggaattcgcc
     6841 gaactgtgcc cctgcgggat cttcaaccag gaaagggcca aggagctctt gattttggag
     6901 aaggaaaatc ctgcttgacc gaaaccttga taatccagaa ggaagccctc ctgaggccgc
     6961 cgtcaggcct atcagaacgg tggccaaagc cccgtttatc gccccccggg catcaagatt
     7021 catctcccct tccaggaata aatttgttat aattcttctc acccaaagtt tcggcgcttg
     7081 attccagctc cacttgcctt gagaacgaaa ttcaaattaa ttaacccggc caagccggga
     7141 ccaaacagga ttttgtaggg gcggtcctgt gtggccgccc gctggatgga cgaccgcgca
     7201 ggaatgcccc tacgatttgg taccctttag cccgtaagtg ccttccggtc tttatcggcg
     7261 aagaacccaa agtggggcct agccatggag gcctcttttg ttttatgaaa atgaagggga
     7321 aagggatgaa gattgatccg gaaaagtaca tcgggcgcga agcctgcatc cctcactgca
     7381 ccgttggagc gattcatctc ggatattttt cttttaaaat tcgatgaaga ggaggagtta
     7441 ccgatgaaaa aactgatttg ggtcggtttt ttgggtcttc tcattctagg aacttcagca
     7501 ggatcggttc tggcccaggg caagtatccc accaaagcca ttgatatcat cgttccctat
     7561 gctcccggcg gcgggaccga catcatgtat cgaaatatcg agaaaatcat cagccagtac
     7621 aagctggttt ccca
//
LOCUS       DASPRR010000319         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1848790, whole genome shotgun sequence.
ACCESSION   DASPRR010000319 DASPRR010000000
VERSION     DASPRR010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848790"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..2632)
                     /gene="nuoD"
                     /locus_tag="VEL68_11915"
     CDS             complement(161..2632)
                     /gene="nuoD"
                     /locus_tag="VEL68_11915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HXZ36716.1"
                     /translation="MGTQEPQKPGKAENLILPRTDSLINWARQYSLWPMFFGLSCCFV
                     EEATAFTPRYDIARFGAEVLRGSPRQADLLIISGTVFKKVAPVILRLYEQMAEPKWVI
                     SMGSCANSGGMYDAYSVVQGIDQILPVDVYVPGCPPRPEALLQGLVLLQEKILSERPS
                     RPVFHLPGGYQGTQKAILVDGQTKSRDPRGPGYNGTAIRGTSATPPQFWDNRGDLMWT
                     PSPRVVELTAPDQSLAAALKSRFGDTISQFPRTSDMLTFQVAENQVKDVLRYLKTEAS
                     PRFLRLDDLTAIDESGRRGDLVQTVREGTDGKEIVLDHPSPAGRQANADYALVYHLLS
                     FDPPTRVRLKVLLQGKDPVSPTITDLWPSANWYEREVFDMFGIRFEGHPNLRRLLMPH
                     DWEGHPLRKDHPGRATEMPPYTHEDARKHQPLDAGLLAGGVNEDGEEFVLNMGPHHPA
                     THGLMRFVVKLRGEEITRLDLDIGYHHRAAEKIGERQSWHQFIPYTDRVDYLSGAANN
                     LSYVHAVETLAGIKVPERAQYIRVLLAELFRLSSHLVFFGTFVQDLGAMTPVFYTFRE
                     REKILDLVELITGGRLHPSWLRIGGVAADLPEGWKEGLEDFIKIFPRRLKEYEALITR
                     NPIFKARTRGVGRLSLQEAMEWGVTGPNLRACGLAWDMRKKFPYAAYEALRFEVPTAA
                     EGDCLARYRVRVEEMRQSVRILEQVVAQMPAGRHITDDYRYVVPDKTDTLKDIESLIH
                     HFIHVTRGPKIPRGEAYASTEAPRGEQSYYVVSDGLSTAYRMRIRTPDFANVQVLPLM
                     AVGGTISDLVAIAGSIDYILPDIDR"
     gene            complement(2587..3015)
                     /locus_tag="VEL68_11920"
     CDS             complement(2587..3015)
                     /locus_tag="VEL68_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXZ36717.1"
                     /translation="MNSINELAIVSPWEPGLLSLALYAFMVSLVVATMLIFATWLGKG
                     QPAPQKPEAYESGIRPTGSARFPFSIPFYLLATFFLIFDVEALYILSWAVAAEPLGWE
                     GWMQITFFIGVLLVSLLYIWKKGGLEWGPKSPKSPERPRI"
BASE COUNT          633 a          895 c          949 g          590 t
ORIGIN      
        1 aagatagcca tagaaattct gcagcgccac cataacatcc acagccatct cccggggatg
       61 ctccgcctca acaatctttt tttgtaggtc agcctgtaat tcgtccggca acataattaa
      121 tcccagctct gacgtcaacc cgaaacccga aactatattt ttatcggtct atatccggca
      181 gtatataatc gatggacccg gcgatggcca ccaggtcgga gatcgtcccc cccacggcca
      241 tgagaggaag gacctgcacg ttggcgaagt ccggagtgcg gatccgcatc cggtaggccg
      301 tgctcaggcc gtcactgacc acgtaataag attgttcccc ccgaggggct tccgtcgaag
      361 catacgcctc tcccctggga atcttggggc cccgggtgac atggataaag tggtggatca
      421 ggctttcgat gtccttcagg gtatcagtct tgtccggcac cacgtagcgg tagtcgtccg
      481 tgatgtgtct gccggcaggc atctgggcca cgacctgctc gaggatgcgg acactctggc
      541 gcatttcttc cacccggacc cggtagcggg ccaggcagtc cccttccgcg gccgtgggga
      601 cctcgaaccg gagcgcctca tacgccgcat agggaaattt ctttcgcatg tcccaggcca
      661 ggccgcaggc cctcaaattg gggccggtga ccccccactc catggcttcc tgcagggaga
      721 ggcgtcccac tccccgggta cgggccttga agatggggtt tcgggtgatc agggcctcgt
      781 attccttcag cctgcgggga aagatcttga tgaaatcctc cagcccctcc ttccaccctt
      841 cgggaaggtc ggcggccaca cccccgatgc ggagccaaga ggggtgcagt cggcccccgg
      901 tgataagctc caccaggtcc agaatttttt ccctctcccg gaaagtataa aaaaccggag
      961 tcatggcgcc caggtcctgg acgaaggtcc cgaagaagac caggtggctg ctaagacgga
     1021 acaattcggc gagaagcacc cggatatatt gagcccgctc aggaactttg atcccggcca
     1081 gagtctccac ggcgtggaca taagaaagat tgttcgcggc tccactcagg taatccaccc
     1141 gatcggtata ggggatgaac tggtgccagg actggcgctc cccgatcttc tcggcggcgc
     1201 ggtggtggta gccgatgtcc aggtccagac gggtgatctc ttctccccgc agtttgacga
     1261 cgaagcgcat cagaccgtgg gtggcgggat ggtggggccc catgttcagg acaaattcct
     1321 ccccgtcttc attcactccc ccggccaaga ggccggcatc caggggctgg tgcttccggg
     1381 cgtcttcatg ggtgtagggg ggcatctccg tagcccgccc gggatggtct ttccgcaaag
     1441 ggtgaccctc ccaatcgtgg ggcatcagca gtcggcgcag gttgggatgg ccttcgaaac
     1501 ggattccgaa catgtcgaag acttcgcgct cataccagtt ggccgagggc cagaggtctg
     1561 tgattgtcgg ggaaaccggg tcttttccct gaaggaggac cttcaggcgg acccgggtgg
     1621 gcggatcgaa agaaagcaaa tggtaaacga gggcgtaatc ggcgtttgcc tgtctccccg
     1681 ccggggatgg atggtcgaga acgatctctt ttccgtcggt cccttcgcgg acggtttgaa
     1741 ccagatcccc ccgccggccg gattcatcga tcgcggtaag gtcgtccaga cgaaggaacc
     1801 ggggggaggc ttcggttttc aagtagcgca gaacgtcctt gacctggttt tcggccacct
     1861 ggaaagtgag catgtctgaa gtccggggga actgggagat ggtatccccg aatctggact
     1921 tgagagccgc ggccagagat tgatcgggcg cggtcagttc aaccacccgg ggcgaagggg
     1981 tccacatgag atccccgcgg ttgtcccaga actgcggcgg agtcgccgaa gtcccccgga
     2041 tagcggttcc gttgtacccc ggcccgcggg ggtcgcggga tttggtctgg ccgtccacga
     2101 ggatggcttt ctgcgtccct tggtaccctc cgggcaggtg gaagaccggc cgggagggcc
     2161 tttcgctgag aatcttttcc tggagcagga cgagtccttg gagcagagct tcggggcggg
     2221 gagggcatcc gggaacgtac acgtccaccg gcaggatttg gtcgatcccc tgaaccacgc
     2281 tgtaggcatc gtacatcccc cccgaattgg cgcacgaacc catggatatg acccatttgg
     2341 gctcggccat ctgttcataa agccgcagga tgaccggggc gaccttcttg aagaccgtac
     2401 ccgagatgat caagaggtcg gcctggcggg gggacccgcg caggacttcg gctccaaacc
     2461 gggcgatatc gtaccgcggg gtgaaagcgg tggcttcctc cacgaaacag caggagaggc
     2521 cgaaaaacat gggccacaga ctgtactgcc gcgcccagtt gatcaaactg tcggtccggg
     2581 gaaggattaa attctcggcc tttccgggct tttggggctc ttgggtcccc actccaggcc
     2641 tccttttttc cagatgtaga gaagactgac gagcaaaacg cctatgaaaa aagtaatctg
     2701 catccaccct tcccatccca gcggctcggc ggcaacggcc cacgagagga tgtagagcgc
     2761 ctccacatca aagataagga aaaacgtggc caggagatag aagggaatgg agaatggaaa
     2821 ccgagctgag ccggtagggc gaatcccgct ctcgtaggcc tcaggcttct gcggggccgg
     2881 ttgccccttc cccagccagg tggcaaaaat gagcatcgtc gccaccacaa gagaaaccat
     2941 aaaagcgtag agagcaaggg ataaaagccc gggttcccag ggagacacga tggcgagctc
     3001 gttgattgag ttcatcggtg cgccttgctg aggcgaatcc ccgccggatc atgacgggag
     3061 atgacgg
//
LOCUS       DASPRR010000320         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_390479, whole genome shotgun sequence.
ACCESSION   DASPRR010000320 DASPRR010000000
VERSION     DASPRR010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390479"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VEL68_11925"
     CDS             <1..639
                     /locus_tag="VEL68_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36718.1"
                     /translation="IDQDKIIKNILEGNAFKLASPLSKYQFGYDPSIKPYDYNPAQAK
                     KLLAEAGYPNGFDLPINSPSGRYLRDKEVVEAIIGDLRKVGINASIRVLEWGSYMTQL
                     YARKLGPAYLLGWGGLTFDADGTFYPLFRTGQALSNFSSPKLDALLEQARSTMDREKR
                     QKLYAEASQVMKEEVPCAFVYQQVDIYGISERLNWKPRPDERLFVFDMDFKE"
     gene            complement(735..1970)
                     /locus_tag="VEL68_11930"
     CDS             complement(735..1970)
                     /locus_tag="VEL68_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ36719.1"
                     /translation="MSETVIFKNGFLIDGRGGEPVPDAAVVVAEKKIQEVLGPGKESR
                     ISNARVIDLKGKTLMPGLIDAHVHVGNIEVVMERTAALPPAVYVHRATRNLETDLDLG
                     FTTLRDAGGLDWGFRAAIEQDLIRGPRVLLSVNMVTQTGGHGDKRGWAKEQQPPRNSI
                     GMYPEVCDGPDEVRRGVRETLRRGADQVKVMADGGVVSPSDKPGQWQYTVEELKAAVE
                     SAEAAKTYVMAHTYTPKAMQNCLRAGVRSLEHGNLLDAETAKMIAEKGAFLVPTLTVF
                     DILAKEGKGSGLDPFILSKLDFVREGMFRALELAWKAGVKIGSGTDIIGPFQYLKGRE
                     LALKAQVMGPLEAIISATRTNAELLCLENEIGTVEAGKLADLIVLEGNPLRDITLFEK
                     GLQKVLLVMKEGRILKDRM"
     gene            complement(2063..2731)
                     /locus_tag="VEL68_11935"
     CDS             complement(2063..2731)
                     /locus_tag="VEL68_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXZ36720.1"
                     /translation="MMLFDLHLHTDYYSSCSAISPEELIRQAAALKLDGIAVTEHGIR
                     WPDDKFDFVRRLAEPHGLILIDAQEVQTYSPKNGMEGEFLVFGLKKSLGSDFSAKALV
                     ERVHGEGGILIAAHPYKWSRFGKDRYYGAGDRIYELQLDGIELLHPDHDEEAITKVRQ
                     AMKKLGLPGTGGSDAHQVHEIGACLTLFANEVRNEADLIREIRAGRIQAVENPMFKYH
                     DTNK"
     gene            complement(3166..>3402)
                     /locus_tag="VEL68_11940"
     CDS             complement(3166..>3402)
                     /locus_tag="VEL68_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36721.1"
                     /translation="QKYRKPVLLGEKGKEQGFYGFGADLHCPFCGGRFDFILIEFENN
                     LDDTPLSWGEIDLVEFNVDLLRCPQCGQRDLEPL"
BASE COUNT          749 a          987 c          845 g          821 t
ORIGIN      
        1 atcgatcagg acaagatcat caagaacatc cttgagggaa atgccttcaa gctggcttcc
       61 cccttgtcca agtaccaatt cggctacgac ccctcgatca aaccgtatga ttacaacccg
      121 gctcaagcca agaagctcct ggccgaagcc ggatatccca acggcttcga cctgcccatc
      181 aactctccca gcggccgtta cctaagggat aaagaggtgg tggaagcgat catcggagac
      241 ctgcgcaagg tgggaatcaa tgcctctatt cgagtgctgg aatgggggtc ttacatgact
      301 cagctgtatg cccggaagct gggcccagct tatcttctgg ggtggggagg attgaccttc
      361 gacgccgatg gaaccttcta tcccctcttc cgcacgggcc aggccctctc caacttcagc
      421 agcccaaagc tggatgcgct gctcgaacaa gcccgttcca ccatggaccg ggagaaacgg
      481 cagaagctct acgccgaggc ctcccaggtc atgaaggaag aagtcccctg cgcatttgtc
      541 taccagcagg tggatatcta cgggatcagt gagcggttaa actggaagcc caggccggac
      601 gagcggctgt tcgtgtttga tatggatttt aaggaataag tttcgagttt caggtttcgg
      661 gtttcgagtt acggattcat gattgccatt cccggtttgc cggttttcaa cccgaaactc
      721 gaaactgcca ttcgttacat ccggtctttc agaatccgcc cctccttcat gaccagaagg
      781 accttctgaa gacccttttc gaagagggtt atatcccgga gtgggtttcc ctccagtacg
      841 atcaggtcgg cgagctttcc tgcctccacc gtcccgattt cattctccag acaaagtagc
      901 tccgcgttgg tgcgggtggc cgagatgatg gcctccaggg gtcccataac ctgggccttg
      961 agggccaatt ctcggccctt caggtattga aatgggccga tgatgtccgt gccggaaccg
     1021 atcttgaccc cagccttcca ggccagctcc aacgcccgga acatcccttc ccggacgaag
     1081 tcaagcttgg aaagaatgaa ggggtccagc cctgatcctt tcccctcttt ggctagaata
     1141 tcgaatacgg tcagggtggg aaccaagaag gcgccctttt cagcgatcat ttttgctgtc
     1201 tctgcgtcca gcaggttccc atgttccagg ctgcgcactc cggcgcggag gcaattctgc
     1261 atggccttgg gggtataggt gtgggccata acataggttt tggccgcctc cgcactctcc
     1321 actgcggctt tcaattcttc taccgtatat tgccattgac ccggtttgtc agaaggggag
     1381 accacccccc catcggccat gaccttcact tgatccgcac ctcttcgcag cgtctcccgg
     1441 acgcctctgc gtacctcatc cggcccgtcg catacctccg ggtacatccc gatgctgtta
     1501 cggggaggtt gttgttcctt ggcccatccc cgtttatccc cgtggccccc ggtctgggtc
     1561 accatgttga cgctgagcag caccctcggt ccgcggatga ggtcttgttc gatggctgcc
     1621 cgaaatcccc agtccagccc tccggcatca cgcaacgttg tgaatcccaa atccagatcg
     1681 gtttccagat tcctcgtagc ccgatgaacg taaaccgccg gagggagggc ggccgtccgt
     1741 tccattacca cttcaatatt tcccacatgg acatgggcgt cgatcaagcc cggcatgagg
     1801 gtcttccctt tcaggtcgat gacccgggca ttcgaaattc ggctttcctt tccgggcccc
     1861 aaaacctcct gaatcttttt ttcggcaacc accaccgctg catcgggtac gggttcccct
     1921 ccccgaccat cgattaaaaa accgttcttg aagatcacgg tttcgctcat gttcgccctc
     1981 tccttgacat aggatgattc tatgactcta gtagggcggt tttgaaaccc gcccccacgg
     2041 gctagaagcc ctcctccgca attcatttat ttgtatcatg atatttgaac atcggatttt
     2101 ccacggcctg aatccgaccg gcccggattt cgcggataag atccgcttca ttccttactt
     2161 cattcgcgaa taaggtaagg cacgcaccga tctcatggac ctggtgggca tcactgccgc
     2221 cggttccggg gaggcccagt ttcttcatgg cctggcggac cttggtaatg gcctcttcat
     2281 cgtgatccgg atgaagaagt tcaatgccat ccagctgcaa ttcgtaaatc cggtcccctg
     2341 caccgtaata acggtccttc ccgaagcgag accacttata gggatgcgcg gcgatcagaa
     2401 ttcctccttc cccgtggacc ctttccacga gagccttggc cgaaaaatct gagcccaggc
     2461 ttttcttcaa accgaatact agaaactcgc cctccattcc gttcttgggg ctgtaggttt
     2521 ggacttcctg ggcatcgatc aaaatgagcc catggggctc agccaacctt cggacgaaat
     2581 cgaatttatc atccggccag cgaatgccgt gttccgtcac cgcaatcccg tcaagcttca
     2641 gggccgcggc ctgacggatc aattcttctg ggctgatcgc cgagcagctg gagtagtagt
     2701 cggtgtggag gtggaggtcg aaaagcatca tgatgagagt gcttaaaatg cctaagattt
     2761 aaaaccgaaa cgagcagcta gtaaccagca actcaaagct caaagctcaa aacccaaaac
     2821 tcgaaacccc agacttctct tattctttta tacccaaaat catgccgaat ataaactacc
     2881 attccttcct catgccgaat tttccaagat tcatttccat tttgtcaagt ttgacgggtt
     2941 atcagggatc atgaagagaa gagcctctga tatcctcaga ctcttggttt tgatcgtggt
     3001 gaagaaatgg ccagcgaaga gttccgtcgg tttttcgatg tcttgtcggc atccctcttt
     3061 ctgctgattt tcttctgggt tctcgctcag gaggaatctc tcgaacaggg caattgaaga
     3121 gtcgagtgag ggaggtttgg ggaggggaac agataaaaaa ggggattaaa gcggctccag
     3181 gtcccgttgc ccgcattggg gacaacgaag aaggtctaca ttaaactcca ccagatcaat
     3241 ctccccccaa gacaaggggg tatcgtccag attgttttca aattcaatca gaatgaagtc
     3301 aaaccttccc ccgcaaaatg ggcagtggag atcggctcca aagccataga atccctgttc
     3361 cttccccttt tcccccaaga gaacgggttt cctgtatttc tg
//
LOCUS       DASPRR010000321         6378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_557869, whole genome shotgun sequence.
ACCESSION   DASPRR010000321 DASPRR010000000
VERSION     DASPRR010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557869"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VEL68_11945"
     CDS             complement(<1..646)
                     /locus_tag="VEL68_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXZ36722.1"
                     /translation="MQQYSVVGQSFPQVDARERVSGMAEFVSDIKLPGMLYGRILRSP
                     HPHARIVHIDASRAKRLAGVKAVVIAEDTPKIPFGPRTDDWMILALDKVRFVGDEVAG
                     VAAVDEDTAEEALSLIQVEYEPLPAVFDPVEAMQPGAPLIHNKERNIVSEFKLSKGDI
                     EKAFQDSDVVHSDRYYTNQVYQAYLEPMACVVQPDISGKLTFYISTQIPSMTRLP"
     gene            complement(688..1152)
                     /locus_tag="VEL68_11950"
     CDS             complement(688..1152)
                     /locus_tag="VEL68_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ36723.1"
                     /translation="MRYPVALTVNGELYELEVPGHRTLLDLIREDLGLTGTKQGCGNG
                     ECGACTVLLDGDPVNACLVLALQADGKSVTTIEGLSKGGKLDAIQEAFIAKGAIQCGF
                     CTPGMVLSVKALLDRNPHPGQSEIREALTGNFCRCTGYQKIVEAVDSLIHHS"
     gene            complement(1178..2065)
                     /locus_tag="VEL68_11955"
     CDS             complement(1178..2065)
                     /locus_tag="VEL68_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXZ36724.1"
                     /translation="MRLRKFEFSSPSSLAEALELMEKHGAGAKLIAGGTDLLVQMKSK
                     TTTPPLVISLLKIPKLSEISPDGKGCKIGALAKHARIGDAPILQERWGILASAARKVG
                     SPQIRNWGTVGGNLCNASPAADTAPPLLVLEAEVILVGRRGERRLPLESFFVGPGVTA
                     LTQDEILKEILIPEAPGPSAWAYLKLGRRKSLDLALSSVAVWLTADPHTKVCCRARMA
                     LGAVAPTPMRAKETEKFLEGKVLEERVILEAGAKAAMECNPISDIRASAEYRREMVNV
                     LVQRAIKKSLGEAIPPTGI"
     gene            complement(2159..2935)
                     /locus_tag="VEL68_11960"
     CDS             complement(2159..2935)
                     /locus_tag="VEL68_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HXZ36725.1"
                     /translation="MMKIASIQMKVEDEKKSERLARAEALLDRAKGSDLVILPEIWNT
                     GYFSFDLYAQEAEPLNGETIQRMSAKAREHGYYLHAGSFVENKDGRLYNTSLLLDREG
                     KRVGIYRKIHLYGYGSRESQILSRGSEVVVENTPLGKIGLATCYDLRFPELFRAMLDK
                     GVELFLVTSGWPYPRLEHWIMLNRVRAIENSAFLISSNCVGINRGARFCGHSMIVDPW
                     GVILSSGGDEECILRSEIDLAKVTAVRGEFPAVNDRILSG"
     gene            complement(2932..3645)
                     /locus_tag="VEL68_11965"
     CDS             complement(2932..3645)
                     /locus_tag="VEL68_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 family protein"
                     /protein_id="HXZ36726.1"
                     /translation="MKTLKFYLEQDGKVEEIFFPLRRMVNAGYTGRDQEKVKRHIEEL
                     KKEGIAAPASAPTAYEVISHLILLDDEIEVIGDKTSGEAEFVLLCRGDDIYVGAGSDH
                     TDRELETVSIIKSKQVCPNVMSSRVWNLKEIRKDWDQIRLRSWAKNEKGEKILYQEAP
                     LSAIMPPEDLLRFVKNALDDRNLDGMAIFSGTVAILPEKIHYSSYFGVELEHPKTGRK
                     LRCDYGVRVLNYLKEGTRG"
     gene            complement(3675..4016)
                     /locus_tag="VEL68_11970"
     CDS             complement(3675..4016)
                     /locus_tag="VEL68_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ36727.1"
                     /translation="MAKPEIEFIDPDTHYSWRPVEGDTLGIKEKILSEDPKTKSYTRL
                     LKFPPGIKTAETLVHDFWEEVWIVEGELIDLAKKQTFCAGFYACRPPGMKHGPYDIPR
                     GCITFEIRYYR"
     gene            complement(4053..4733)
                     /locus_tag="VEL68_11975"
     CDS             complement(4053..4733)
                     /locus_tag="VEL68_11975"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXZ36728.1"
                     /translation="MPRKLPEDFAQFQRPRPLNDMAYETIKSSILEGKLISGEIYSEL
                     DLAKSFGISRTPVREAFLRLAAENLIIFHPRKGISVNYFSKEDIENLFELRQAIEEAT
                     ANKIVGNLSKDQVRYIQETIRQQEACIKDKDYDENLFLEFDRKFHLFFVEISGNRFMV
                     QTYNNIRDYITIPAREALRKKGRAKEVLSEHKAIVEALSGEDAERTKEAIKIHLINSK
                     LAALECRQ"
     gene            complement(5186..5887)
                     /locus_tag="VEL68_11980"
     CDS             complement(5186..5887)
                     /locus_tag="VEL68_11980"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXZ36729.1"
                     /translation="MLFKGKKIIDLSQDIYTGMPVYPGHAKTVIWEHASHEETKRMLG
                     TGFSYRSSGIMMCDHGPTHVDSVSHLSPDPQAPSVDEIPLEHCVTSAICLDVSDVPVQ
                     TQFGPEKIETQLKKWKLDIRPGDVVLFYTGHYEKYYGKPEYSSHQPGLTRAATEYILD
                     RGAISFGVDNTSPDMFLDKTYPCHMVCRERKITHYENLCNLDKLVGKRFTFIALPLKI
                     RKGTGSPVRPVAILD"
     gene            complement(5986..>6378)
                     /locus_tag="VEL68_11985"
     CDS             complement(5986..>6378)
                     /locus_tag="VEL68_11985"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXZ36730.1"
                     /translation="KLIQVYEIGKAINPLLCRGQINGGAMMGIGFALTEDFTTYYPSM
                     EHAPDNLADYTIATAADMPREMVWDIVEVPHPNGPRGAKGFSEMTASAAPPAILSAIH
                     NAIGVWVTDYPATPERILRALEEKKAKG"
BASE COUNT         1365 a         1777 c         1647 g         1589 t
ORIGIN      
        1 aagggagtcg ggtcatggag ggaatctggg tggagatgta aaaggtcagt ttcccggaaa
       61 tatccggctg gactacgcag gccatgggtt ccaggtatgc ctggtagacc tgattggtgt
      121 agtagcggtc cgaatggacc acatcgctat cctgaaaggc cttctctatg tctcccttgg
      181 agagcttgaa ctcgctgacg atgttccttt ctttattgtg gatcaggggg gcgcccggtt
      241 gcatggcttc gaccgggtcg aagacggcag gaagcggctc gtattcgacc tggattagcg
      301 aaagagcctc ctccgccgta tcctcatcga ccgccgccac cccggccacc tcatctccca
      361 caaatcggac cttatccaag gccaggatca tccagtcatc ggttctcggg ccgaaaggaa
      421 ttttgggcgt atcttcggcg atgaccacgg ccttcacacc cgccaggcgt ttggccctcg
      481 aggcgtcgat gtggacgata cgggcatggg gatgagggct gcgcaggata cgcccataga
      541 gcatgccggg cagcttgatg tcggacacaa attcggccat ccccgagact cgttcgcggg
      601 catcgacctg ggggaaggac tgaccaacga ccgaatactg ttgcataaga aacctctttt
      661 attagatggg cgatgatcca atggccccta ggaatgatgg attaaagaat cgacggcctc
      721 cacaattttc tgatagccgg tacagcggca gaaatttccc gtaagggcct cgcgaatttc
      781 cgattgcccg ggatggggat ttcggtccag caaagccttg acggagagca ccatccccgg
      841 cgtgcaaaag ccgcattgga tggctccctt ggcgataaag gcttcctgga tggcgtccag
      901 ctttcctcct ttggataggc cttcaatcgt ggttaccgat tttccatccg cctggagggc
      961 taaaaccaaa caggcgttca ccggatcgcc gtcgaggagc acggtacagg ctccgcactc
     1021 cccattaccg cacccttgtt tagtccccgt gagtcccaaa tcttcccgga ttaggtctaa
     1081 aagagtccga tggcccggga cctccagttc gtagagttcg ccattcaccg taagcgccac
     1141 aggatatctc attcgatcct ccttatccat gaggccgcta aattcccgta ggaggaatcg
     1201 cctcccccaa ggattttttg atcgccctct ggacaagaac gttgaccatt tctcttcgat
     1261 attcggcaga agcacggatg tcggaaatcg gattgcattc catggcggct tttgccccgg
     1321 cttccagaat tactctttct tccaggactt tcccttccag aaacttctcc gtctctttgg
     1381 ctctcatggg tgtcggagcg acggcaccca aggccatgcg cgcccggcag caaaccttcg
     1441 tgtgcgggtc tgccgttaac cataccgcca cgctgcttaa ggctaaatcc aggctcttcc
     1501 gccgccccaa cttcaggtag gcccatgcgc ttggaccagg ggcctccgga atcaagatct
     1561 ctttcagaat ttcatcttgg gtcagagccg tcacgccggg gccgacgaag aaagattcca
     1621 ggggcagacg gcgctctcct ctccggccga cgagaatgac ctctgcttcc aaaaccagca
     1681 gcgggggagc cgtatccgca gccggggaag cgttgcataa atttcccccg acggttcccc
     1741 aattgcggat ctggggagaa cccactttcc gcgccgcaga agccagtatg ccccatctct
     1801 cttgcaggat cggagcatcc ccgatccggg catgtttggc cagggcgccg attttacacc
     1861 ctttcccgtc tgggctgatc tcggagagtt tcggaatttt cagaaggctg atcaccaagg
     1921 gaggcgtagt ggttttggat ttcatctgaa ccagcaggtc tgtaccgccg gcaatcagct
     1981 tggccccggc cccatgcttt tccattaact ccaaagcttc tgccagggag gaaggagagg
     2041 aaaactcaaa ttttctcaga cgcatcgctc actccttgaa aaattagcgt gttctcggac
     2101 cgccgattct tccctggaaa accccgaaga gaaaaaatgg tgaagggtaa aatatgcttt
     2161 atccactcaa aatccggtca ttcacggccg ggaattctcc ccttaccgcc gtaactttgg
     2221 cgaggtcaat ttcagaccgc aagatacatt cttcgtctcc tccgctggac aggatgactc
     2281 cccagggatc aacgatcatg ctgtgcccgc agaaacgggc tccccgattg atgcctacgc
     2341 aattgctgga aatcagaaaa gccgagttct caatggcccg gactcgattc agcatgatcc
     2401 agtgttccag gcggggatag ggccaccccg aggtcaccaa aaacaactcc acccccttgt
     2461 ccagcatggc ccggaataat tccgggaatc gcaaatcgta gcaagtcgca agaccgattt
     2521 tccccagggg ggtattttcg accacgactt cgcttccccg ggaaaggatt tggctctccc
     2581 tcgatccata cccgtacaga tgaattttgc gatagatgcc tacccgtttc ccttcgcggt
     2641 ccaggagcag actggtattg tatagccggc catccttatt ttccacgaaa cttccggcat
     2701 gaaggtaata accgtgttcc ctggccttgg ccgacatcct ctggatggtc tctccattca
     2761 ggggctccgc ctcttgggcg taaagatcaa aggaaaaata gccggtattc caaatctccg
     2821 gaaggatcac caggtccgag cctttcgccc ggtccaatag ggcttcggcg cgagcgagcc
     2881 tctccgattt cttttcatct tctaccttca tctggatcga agcgatcttc atcatcccct
     2941 cgtgccttct tttagatagt tcagtactct taccccgtaa tcgcagcgga gctttcttcc
     3001 tgttttggga tgctccagct caactccgaa atagctgctg taatggatct tttccggaag
     3061 gatggccacg gttccggaaa agatggccat cccatccagg ttgcggtcgt ccagggcatt
     3121 tttaacgaac cttaagaggt cctcgggggg catgatggcg gacagagggg cttcttgata
     3181 caggatcttt tctccctttt cattctttgc ccaacttcgg agccgaatct gatcccaatc
     3241 cttccggatt tccttgaggt tccacacccg agaagacatg acattgggac agacttgctt
     3301 tgatttgatg atgctgactg tttccaactc ccggtcggta tggtcgctgc ctgcccctac
     3361 gtagatgtca tctccccggc ataaaaggac gaactctgct tccccagagg tcttatctcc
     3421 gatcacttca atttcgtcgt cgaggaggat caggtgggag atgacctcat aggcagtggg
     3481 ggcggaggcg ggcgccgcga tcccctcctt tttgagttct tcgatatgtc ttttcacctt
     3541 ctcctggtcc cggcccgtgt atccggcatt gaccatcctc cttaaaggga agaagatttc
     3601 ttccactttc ccgtcctgtt ctaaataaaa tttaagcgtt ttcactgcag gttcctccgc
     3661 gaaaagaaaa aattctaccg gtaatatcgg atttcgaagg ttatgcaacc ccggggaata
     3721 tcataggggc cgtgcttcat tcccggtggg cggcaggcat aaaacccggc acagaaggtt
     3781 tgtttcttgg ccaaatcgat cagttctcct tccacgatcc atacttcttc ccaaaaatca
     3841 tggaccaagg tctcggcggt tttgattccg gggggaaatt ttaggagtcg ggtgtaagac
     3901 ttcgttttcg ggtcttcgct caagatcttc tccttgatcc ccagggtatc gccctccacg
     3961 ggtctccagg aataatgggt atccgggtcg ataaattcaa tctcgggctt agccatgctg
     4021 gttttctcct ttctcggaat aatatttctc ttttactggc ggcattctaa ggccgccaac
     4081 ttgctgttga tcagatggat tttaatggct tctttcgttc tttccgcatc ctctccggat
     4141 aaagcctcca caatcgcttt gtgttcgctc aagacttcct tggcccgccc ctttttcctc
     4201 agcgcctccc tggccggaat ggttatgtag tctcgaatgt tattataggt ttgaaccatg
     4261 aagcggtttc cgctgatttc aacgaaaaaa agatgaaatt tccggtcaaa ctccaaaaaa
     4321 agattttcat cataatcttt atcttttatg caggcttcct gttgccttat ggtttcctgg
     4381 atgtaccgca cctgatcctt ggacaggttt cccacgatct tgttggccgt tgcctcttca
     4441 atggcctgcc gcagctcaaa taaattttca atatcttctt tgctgaaata attgaccgaa
     4501 atccccttcc ggggatgaaa aatgatcaga ttctccgcag cgagcctcag gaaggcttcc
     4561 cggaccgggg tcctagaaat tccaaagctt ttcgccaggt cgagctcgct gtagatttcc
     4621 ccgctgatca gctttccttc cagaatgctg gatttgatcg tttcataagc catgtcattc
     4681 aagggccggg gtctttggaa ttgagcaaag tcctcgggta attttctggg catcttaatt
     4741 tttgggtctc ctgtgaagga agcttgtata cggtatctta aatactgtat ctcaccttaa
     4801 atgtcaatgg agaaatttac cggaagggaa actccttccc cttcaccccc caccctgtcc
     4861 cttcatcgcc agggaggagg gaatttttct ttatatttca acaaattttc cctcttcacg
     4921 cctcggcgtg ataggaaagg tcagggctgg ggtggggtat ctcatacacc cgttagagtg
     4981 caggatcggg ggcttgacga aggaaaggtg aagatcggag ggccggcaag gactggatgc
     5041 taatcgacgg gaccagagtc aggcgaattt caggttctaa cccaactgtt caataaaggc
     5101 gcatggcaga gacagggcgg gaaggaggcc atcttccaac ggattaagat ggcctccccc
     5161 cagttaagag tgggaaatgg aatcactagt ccagaatggc taccggccgc accggcgacc
     5221 cggttccctt gcggatcttc agcggcagag cgatgaaggt gaaccgtttg cccaccagct
     5281 tatccaggtt gcagaggttc tcatagtgag tgatcttccg ctcccggcag accatgtggc
     5341 agggataggt cttgtccagg aacatatccg ggctggtatt gtccaccccg aaactgatcg
     5401 ctccccggtc gaggatgtat tcggtggccg cccgggtcaa cccgggttga tggctggagt
     5461 actccggttt tccatagtac ttttcataat ggccggtata gaacagaacc acgtcccccg
     5521 ggcgaatgtc cagtttccat tttttcaatt gcgtttcgat cttctccgga ccgaactggg
     5581 tctggacggg aacgtcactc acgtcgaggc aaatggccga ggtgacgcaa tgctccaggg
     5641 ggatttcatc caccgaggga gcctgggggt ccggcgagag atggctcacc gaatccacgt
     5701 gggtcggacc gtgatcgcac atcatgatcc cgctggaccg ataggaaaat cccgttccca
     5761 gcatccgttt ggtttcttca tgggatgcgt gttcccaaat gaccgttttg gcatgcccgg
     5821 gatataccgg catgccggtg taaatgtcct gactcaagtc gatgattttc tttcccttga
     5881 aaagcatttc tcgatccccc tcgaatttct tggaggggcg gggaattccc ccgcccccga
     5941 tgaagtcctg acccccggca gaaccgggga tttaccttaa ggatattagc ctttggcttt
     6001 cttttcctct aaagccctca ggatgcgttc gggcgtggcc ggataatcgg tgacccaaac
     6061 cccgatggca ttgtggatgg ccgacaggat ggcgggcggg gccgcgctcg cagtcatctc
     6121 cgaaaatcct ttggcccccc gcggcccgtt gggatgggga acctcgacga tatcccagac
     6181 catctcccgg ggcatgtctg cggcggtggc gatggtataa tcggcaagat tgtcgggcgc
     6241 atgttccatg ctgggatagt aagtggtgaa gtcttcggtc aaggcaaacc cgatccccat
     6301 catggcccct ccgttgatct ggccccggca aagaagcggg ttgattgctt tgccgatctc
     6361 atacacctgg atcagctt
//
LOCUS       DASPRR010000322         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1849822, whole genome shotgun sequence.
ACCESSION   DASPRR010000322 DASPRR010000000
VERSION     DASPRR010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849822"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1594)
                     /locus_tag="VEL68_11990"
     CDS             complement(194..1594)
                     /locus_tag="VEL68_11990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXZ36731.1"
                     /translation="MKKFVWVALGILGILGVAGGVYVYLVFFEQEKPTIQVLPDSRYI
                     GKELILKVQDQKSGVAELQVDVIQAGRPINLLSEKFPKKTLLVERTIPLRPLPQGLRE
                     GEAQIKVSAKDHSWNWGNPVSVEKPVVIDITPPQLTVLGTLHYANQGGTGVITYQVSE
                     EVPLSGVQVGQTFFPGYAVAQGRYVAYFAIPLDASSKTPTSVLAEDPAGNRSQEGFRV
                     IVKPRAFKRDKIEITDPFLQSLLPYFTSRDPNLKGTPLEMFLEVNRKQREVDHQEIKK
                     ISKETAPKSLWSGTFLRLPNSKPMASFAQDRTYQYNGKEVDRQIHWGVDLASLAQSPV
                     PASNSGIVVFAGPLGIYGNTVVIDHGCGLFSMYSHLSKIETEVKREVKKGDTLGRTGS
                     TGMAGGDHLHYAMMIQGVFVNPLEWWDEHWIRDNVDLKLKGFDGQPKASPPKQTAVHA
                     KPKAKPKKPTLKAGKP"
     gene            1732..2310
                     /locus_tag="VEL68_11995"
     CDS             1732..2310
                     /locus_tag="VEL68_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ36732.1"
                     /translation="MRVVALNGSARKDGNTAILLNLVLDELQAEKIETEMIQLAGEKI
                     SGCIACYKCQKNKDGKCVVKADRANEYIRKMREAEGILLGSPTYVADITANLKAILER
                     ATIVCRANGGEMLKRKPGAGIVAVRRAGAIQAINVLNAFFLINQMIVVGSSYWNVGIG
                     RNLGEVNSDLEGIQTMKALGQNMAWLLKKIHG"
     gene            complement(2440..>2684)
                     /locus_tag="VEL68_12000"
     CDS             complement(2440..>2684)
                     /locus_tag="VEL68_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXZ36733.1"
                     /translation="RREFLDKFPSNWELSAARATTVVRYFQDKSGLDPKGMEAVGRSF
                     YQPAAANDNEKNRALNRRVEILIAPVMELKEKEKEK"
BASE COUNT          589 a          746 c          728 g          621 t
ORIGIN      
        1 aaaatcgccc ggcggctggt caaggaaaaa tactgggcgg gcagagaccg gagagtaaat
       61 taaaaaaagg agtcctccgg tttacttgct tcaggggtct gcaaggggat aaattctcgg
      121 gcagacctgc acgcaatgag acataccgcc gtcacagata aggttgatgc cggaaatatc
      181 cccgcccttt tgttcaaggc tttcccgcct tcagggtggg cttttttggc tttgccttcg
      241 gctttgcatg aacggccgtc tgcttgggag gtgaggcctt gggctgcccg tcgaacccct
      301 tcaatttcag gtccacattg tcccggatcc agtgttcgtc ccaccactcg aggggattca
      361 cgaatacgcc ctggatcatc atggcgtaat ggagatgatc gcccccggcc attcctgtcg
      421 accccgtgcg gcctagggtg tcccccttct tcacttccct ttttacctct gtctctattt
      481 tgctcaggtg ggagtacatg ctgaagagcc cacatccatg atcgatgacc accgtattcc
      541 catagatccc caaaggcccg gcaaaaacca cgatcccgga gttggacgcc ggcaccgggc
      601 tctgagcaag ggaagcgagg tcaacacccc agtggatttg tcgatcaacc tccttcccgt
      661 tgtactggta cgtcctatcc tgggcaaagg aggccatggg tttggagttg ggaaggcgaa
      721 gaaaggttcc tgaccacaag gatttgggag ccgtttcctt gctgattttt ttgatttctt
      781 ggtgatccac ctcccgctgc ttccggttca cctccagaaa catttccagg ggcgtacctt
      841 ttaggtttgg gtctctggag gtaaagtaag ggaggaggct ctgcagaaag ggatcagtga
      901 tctcgatttt gtctcttttg aatgctcttg gcttgacgat cacccggaat ccttcctgcg
      961 agcggttccc tgccgggtct tcggccaaga ctgaggtagg cgtcttcgaa gaagcatcca
     1021 ggggaatggc gaagtaggct acatacctgc cttgggccac ggcatagcct gggaagaagg
     1081 tttgacccac ctggactccg cttaggggaa cttcctcgga tacctggtag gtaatgaccc
     1141 ccgttccccc ctgattggca taatggagag tccccagcac agtcagctgc ggaggggtga
     1201 tatcgatgac cacgggtttt tccaccgaga ccgggttccc ccagttccac gagtggtctt
     1261 tggctgaaac cttgatctgg gcctcgccct ctctgagccc ctggggcagg ggccgcaggg
     1321 gaatggttct ctccaccaga agggtcttct tggggaattt ttcggagagc aggttgatgg
     1381 gcctgcctgc ctgaatcacg tcgacctgca attcggccac tccgctcttc tgatcctgga
     1441 ctttaagaat cagttccttg ccgatatacc tcgaatccgg gaggacctgg atcgtgggtt
     1501 tctcctgctc aaagaaaacc agatacacat aaacgccccc tgcgactcca agaatcccta
     1561 gaatccccaa agcaacccag acgaattttt tcatcttgtc ctactcttcc tccttccctt
     1621 tcttccgggg accatccttt cttatcctca taataatccc aagccttgcg ggaagccaag
     1681 agataagtta atctgattcc tatagaaaat tttcggaaaa ggagaaggag aatgagagtc
     1741 gtcgctctca acggaagcgc ccgcaaagac ggcaacacgg ccatcctgtt gaatttggtg
     1801 ctggatgaac tgcaggcgga aaaaatcgag acagagatga ttcaactggc aggagagaaa
     1861 atctccggct gcatcgcctg ctacaagtgc cagaagaaca aagacggaaa gtgcgtcgtc
     1921 aaagctgatc gggcgaacga atacatccgg aagatgcggg aggctgaggg gatccttttg
     1981 ggctctccca cttacgtcgc cgacatcacc gcgaacctaa aggcaatcct ggagagggcc
     2041 accattgtat gccgggccaa cggcggggag atgctcaaaa ggaaaccagg agcgggcatt
     2101 gtggccgtcc gccgggccgg cgccattcag gccatcaacg ttctgaacgc attctttctc
     2161 atcaaccaga tgatcgtggt ggggtcgagt tactggaacg tgggaatcgg ccggaacctc
     2221 ggggaagtca acagcgatct ggaaggcatt caaaccatga aggccctggg tcagaacatg
     2281 gcctggctgc tcaaaaaaat ccacggctaa tgcaatggtc ggtcccctcc gccctcacca
     2341 aaaaaatccc gttgccgaaa ttctccccga gcccctcttt gcagggggga ttcggggaga
     2401 ttttttcagg attctagacg ccgtggtttt ccgaaccctt tatttctctt tttccttctc
     2461 cttcaattcc atcacggggg cgatgaggat ctccacccgc cggttcaagg ccctgttctt
     2521 ctcgttgtcg ttggcggccg ccggctggta gaaggagcgc cccaccgctt ccatcccttt
     2581 cgggtctaac ccgcttttat cctgaaaata gcgcacaaca gttgtcgccc gggcggccga
     2641 aagctcccag ttggagggga acttgtcgag gaattcccgg cgga
//
LOCUS       DASPRR010000323         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_558461, whole genome shotgun sequence.
ACCESSION   DASPRR010000323 DASPRR010000000
VERSION     DASPRR010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558461"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VEL68_12005"
     CDS             complement(<1..121)
                     /locus_tag="VEL68_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXZ36734.1"
                     /translation="MPFDQIRGQEKAIAILRNALGNDRLAHAYLFIGPEGVGKH"
     gene            complement(125..760)
                     /gene="tmk"
                     /locus_tag="VEL68_12010"
     CDS             complement(125..760)
                     /gene="tmk"
                     /locus_tag="VEL68_12010"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXZ36735.1"
                     /translation="MSIFITFEGIEGCGKTTQIGHLTSFLIKYKRPFLLTREPGGTQI
                     GEKIRQILLSSDNAGMEPMTELFLYVADRIQHYRQVIAPALREGKAVLCDRFADATTV
                     YQGFGRGLDLVWIEEIHARVLDNIRPDLTFLLDLPVEVGLRRAWKRLENDKTQEDRFE
                     KEALDFHQRIRNGYLALARNEPGRIIVLDGMKDEEALHREIVSHLPGSLRG"
     gene            complement(1196..1618)
                     /locus_tag="VEL68_12015"
     CDS             complement(1196..1618)
                     /locus_tag="VEL68_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ36736.1"
                     /translation="MKVTRFDHITIAVRDLDAAIETFTRCFNLKAKDRRRVNHLGMEN
                     VFLPFGDGAIELAAPLKDGEAVDHVQRFLERRGEGMMNLCLTVEDVDAAVVHLKRCGV
                     RVLEHKDADGDQIAMVHPKDVHGVMIEIRRGKRVIRPS"
     gene            complement(1632..2072)
                     /locus_tag="VEL68_12020"
     CDS             complement(1632..2072)
                     /locus_tag="VEL68_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HXZ36737.1"
                     /translation="MPKKVLFSEDISEGQEAAPLVMPPITRTQIVKYAGASGDFNPIH
                     HDELYAIRAGNDRVFAMGMMTAGFLSHMITDWVGDGKLRKYRVRFGRQVWPGDTITCK
                     GRITRKYTEGAKNYMEAEVFAENQRAEKVITGTIVAEVPSRRAA"
     gene            complement(2083..2544)
                     /locus_tag="VEL68_12025"
     CDS             complement(2083..2544)
                     /locus_tag="VEL68_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ36738.1"
                     /translation="MSIQDWIGKELPSFEMPVERGKIREFANAIGDDNPIYHDPSYAA
                     KSEFAAILAPPTFAATKAFWRIGQSASDLAGLDPRFRLHGEEEFEYFHPVLAGDVLTC
                     RVRVSDAYEKPGKRGGKMTFVVVEYAFCNQKGEKVLVSRTTTLHTDGAVKN"
     gene            complement(2593..3366)
                     /locus_tag="VEL68_12030"
     CDS             complement(2593..3366)
                     /locus_tag="VEL68_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ36739.1"
                     /translation="MEFKNILWEKREGVGIVTLNREDVRNALKAEVRQDIATSLQVAE
                     NDPEVKVVIITGAGRKVFSAGGDIKQMAENTMWDVLERKPDIFAQIHNLSKPVIAAIN
                     GLALGGGCEMAMACDFRVASENARIGQPEINLGIFPGGGATQRLQKLVGIGRAKELIF
                     TGDILDAREAERIGLINRVFPADDLMNRTMDIARKIADKGPLALKLAKMAMNMGMETG
                     LAIGLGYERLARTLVHGSDDRIEGMNAFIEKRKPQFKGR"
     gene            complement(3622..4416)
                     /locus_tag="VEL68_12035"
     CDS             complement(3622..4416)
                     /locus_tag="VEL68_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ36740.1"
                     /translation="MQYSTLLLEKVDEIVLLKLNLPEALNPMNHEMSLEFPQAIEEIR
                     RDPEVKGLILTGNGQAFSAGGNLKIMQQRTEIPPFRHKEEVRQFYRSFLTIRTLTIPT
                     IAAVNGYAIGAGACLALACDLRIASEKAKFGFTFVKIGMHPGMAATYLLPRIVGAAKA
                     YELLATGDLIDASEAHRIGMVNKVVKPEELMPAAREIAGKIAAMPMVAAKTMKESIYI
                     NLEEPSIEVALDREAAHQSLTFTTNDIKEGIAAAIEKRKAKYSDNI"
BASE COUNT          921 a         1323 c         1179 g         1098 t
ORIGIN      
        1 ggtgtttgcc caccccttca gggccgatga aaaggtaggc atgggccagc cgatcattgc
       61 ccagggcatt gcgcagaatg gctatggcct tttcctgtcc tctgatctgg tcaaatggca
      121 cggttcaacc tcgtagggaa ccggggagat gagagacgat ctcccgatga agagcctctt
      181 cgtctttcat tccgtccagg acgatgatcc gaccgggttc attccgggct agggctaaat
      241 aaccatttct tatccgctga tgaaaatcaa gagcttcctt ttcaaaccgg tcttcttggg
      301 ttttgtcatt ctccagcctt ttccaagccc ttctcagccc gacctccacc ggcaggtcta
      361 aaaggaaagt caggtccggt cttatattat ccaggacccg ggcatggatc tcttcaatcc
      421 agaccaagtc cagaccccgc ccaaatcctt gataaactgt tgtggcgtcg gcaaaccggt
      481 cacacaggac ggcttttcct tcccggaggg cgggagcgat tacctggcgg taatgttgaa
      541 tccggtcggc gacgtagagg aaaagttcgg tcatgggctc catgccggca ttgtcggatg
      601 aaaggagaat ctgccggatc ttttctccta tttgggttcc tccgggttcc cgggtgagca
      661 ggaaaggacg tttatatttt attaaaaaag aggtcaagtg accgatctgg gtggtcttgc
      721 cgcacccctc aatcccctcg aaggttatga aaatactcaa gtttccccca atttttgaat
      781 aaatttattc tactctaaaa aatctcattt agcaaatcac cgagtcgcag atcgccgctg
      841 ctaaggtgga aggggataag ggcttgctgg caatcgaagc cggaatgaaa gaaacttctc
      901 cgaggacttt ttccagcaaa ccaggagaga accgcgggca ggaaatttaa gtgctgcttt
      961 cttctggaag aacgggggta tttgaggggc ttgggatccc tcgggcttgg attcccggga
     1021 aaaccttcac ccctcgggcg cgttttgcaa atagggccaa aaatgtattg acgctaaaga
     1081 ctaagccgtt cgtgctgagc ttgtcgaagc atgaacggct tagcgatgca cccttcgaca
     1141 agctcagcac gaacggtgac agcccgagta gacaaggaaa accagaaggg gcccttcacg
     1201 aaggccggat cactcgcttt ccccgacgaa tctcgatcat caccccgtgt acatccttgg
     1261 gatgaaccat ggcgatctga tcgccgtcgg catccttgtg ttccaagaca cgaactccgc
     1321 atctttttag gtggacaacg gcggcgtcca catcttccac ggtgagacag aggttcatca
     1381 tcccctcccc ccgtcgctcc aaaaaccgct gcacatgatc gaccgcttct ccatccttca
     1441 agggagccgc gagttcgatg gctccgtcgc cgaagggcaa gaagacattc tccatcccca
     1501 gatgattcac ccggcggcgg tctttggcct tcaggttgaa acagcgggtg aaggtttcta
     1561 tggcggcatc caggtcgcgg acggcgatgg ttatgtggtc aaagcgggtg accttcattc
     1621 tcttccctcc ttcaagccgc tctccggctg gggacttcgg ccacgatggt tccggtgatc
     1681 accttttcgg ctctctggtt ctccgcgaag acctcggcct ccatatagtt tttcgcgcct
     1741 tcggtgtact tccgggtaat ccgacccttg caggtgatcg tgtccccggg ccagacctgg
     1801 cggccaaaac ggacccggta cttgcgcagc ttcccatctc ccacccagtc ggtgatcatg
     1861 tggctgagga agcccgcggt catcatgccc atggcgaaga cccggtcgtt gcccgcccgg
     1921 atggcataca gttcgtcatg atggatcggg ttgaaatctc cggaggcgcc ggcatacttc
     1981 acgatctggg tgcgggtgat cggtggcatc accagggggg ctgcttcctg tccttcggag
     2041 atatcttcgg aaaaaagaac cttctttggc acgagtcacc tcctaattct tgaccgcacc
     2101 atcagtatga agggtggttg tacgggacac cagaaccttt tcccctttct gattgcaaaa
     2161 ggcatactct actaccacaa aggtcatctt tcccccgcgc ttgccgggtt tctcataggc
     2221 atcggagact ctgaccctgc aggtcagcac gtctccggcc aggaccgggt ggaagtattc
     2281 gaattcctcc tctccatgaa gacggaaacg ggggtccaag ccggccaagt cggaggccga
     2341 ctgccctatg cgccagaagg ctttggtggc cgcaaaggta gggggcgcca ggattgccgc
     2401 gaattcgctc ttggccgcgt aggacgggtc gtgataaatc gggttgtcat caccgatggc
     2461 gttggcgaat tccctgattt tcccccgctc cacgggcatt tcaaaagagg gcagctcttt
     2521 gccgatccaa tcctgtatgg acatagttca atccttccca ttgagtttta ctacggctgg
     2581 tgaccagcct cctcaacgcc ccttgaactg aggcttcctc ttttctataa aggcgttcat
     2641 cccttcgatc cggtcgtcgc ttccatgaac gagggtacgg gccagcctct cgtatcccaa
     2701 gcctatagcg aggccggtct ccattcccat gttcatcgcc attttcgcca gtttcagggc
     2761 caacgggccc ttgtcggcta tcttcctggc aatgtccatc gttcggttca tcaaatcatc
     2821 tgcgggaaat actctgttga tcagaccgat gcgctcggct tcccgggcgt cgaggatatc
     2881 tccggtgaag atgagttctt tagccctccc gatccccacc agtttctgca acctctgcgt
     2941 ggccccgccg ccgggaaaga ttcccagatt gatttccggc tgcccgatgc gggcattctc
     3001 gctggccacc cggaaatcac aggccatggc catctcgcat cctcctccca gggccagacc
     3061 gttgatggcg gcgataaccg gttttgacaa attgtggatt tgggcgaaga tgtcgggctt
     3121 cctctccaac acgtcccaca tcgtgttctc tgccatttgc tttatgtccc ctccggcgct
     3181 gaaaaccttc cggcccgccc cggtgatgat caccactttc acctccgggt cattttcggc
     3241 aacctgcagg gaggtggcga tgtcctggcg gacctcggcc ttcaaggcgt tgcgcacatc
     3301 ctcccggttg agggtgacga tccccacccc ttctcttttt tcccaaagaa tatttttgaa
     3361 ctccatcctt ccctcttctt tcctggacgg gactacatcc cgcctgactc ggcagcgatt
     3421 ttacacaggg cacgctgcac cgcacctctg cagaaattgg gtaacatcaa cgcctcctgc
     3481 aaaactccta agttaatagg aaatcgtcat tccgggcaag cgaagcgcga cccggaatcc
     3541 aggattttaa aagcattctg gattccggct tccgccggaa tgacggagta agtgactttt
     3601 gcaagagcct cggtagaaat cctaaatatt gtccgaatat tttgcctttc tcttctcgat
     3661 ggccgcggca atcccctctt taatgtcgtt cgtagtgaaa gtcagggact ggtgcgccgc
     3721 ctcccggtcc agggccactt cgatgctggg ttcttccagg ttgatataaa ttgattcttt
     3781 catggtcttt gctgcaacca tgggcatggc agcgatcttc ccggctattt cccgggctgc
     3841 gggcatcagc tcttccggtt tgacaacttt gttgaccatg ccgatgcggt gagcctcgga
     3901 agcgtcgatc aggtctcccg tggccagtag ctcataggct ttcgccgccc ccacaatccg
     3961 gggaagaaga tacgttgccg ccatccccgg atgcatgccg atcttgacga aagtgaagcc
     4021 gaactttgct ttctccgaag cgatccgcag gtcgcaggcc agggcaaggc aggcgcccgc
     4081 gccgatagca tatccattca ctgcggcaat cgtcgggatc gtgagggtgc ggatcgtcaa
     4141 gaagcttcgg taaaactggc ggacctcttc tttatgccgg aacggcggga tttccgtcct
     4201 ttgctgcata atcttgaggt ttcccccggc gctgaaggcc tgcccgtttc cggtcaggat
     4261 caagcccttg acctccgggt cccgtctgat ctcctcgatt gcctggggaa actccaggct
     4321 catctcatgg ttcatggggt tcagagcttc cggaagattc agcttgagca ggacgatctc
     4381 atccactttt tccaaaagca gggtcgaata ttgcatcttc tttcctccca tcggaaatga
     4441 gtttggctca ttccaactcc ccgtccagat ccttccactc tctccgtttc cacccgggca
     4501 ggttaggaag gatcaggagg a
//
LOCUS       DASPRR010000324        12003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2010310, whole genome shotgun sequence.
ACCESSION   DASPRR010000324 DASPRR010000000
VERSION     DASPRR010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010310"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1669)
                     /locus_tag="VEL68_12040"
     CDS             complement(197..1669)
                     /locus_tag="VEL68_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXZ36741.1"
                     /translation="MIDLSKNERKLEQAVQRARERKILIPTFQEQIHPECIPGKVKDR
                     LRSIGLWDVNPLNLFRITWKNEPVPSGGGFGKVNYLEFPPELTGVDARIIALVGKWFP
                     TGSHKVGATYGCLVPRLVTGQFDPTGQKAAWPSTGNFCRGGAYNATLLGCESIAILPE
                     GMSRERFQWLATVAGEVIATPGCESNVKEIFDKCWELRQTRGNVVIFNQFDEYGNHLW
                     HYQVTGRALEEVLSAELGEGGRCAGFVSQTGSAGTIAAGDYLKERFLGSRIVASEALQ
                     CPTMLLNGYGDHRIEGIGDKHIPWIHNVRNTDVVTAIDDEACVSLVRLFNEPVGRKYL
                     EPRGVSRDFIPRLSLLGISGIGNLLSAIKFVKYYALQKRDLVLTVLTDSMDLYGTRVE
                     EMQVARGPYTEVQAAADYHRYLLGEGTDNMLELSILDRRRIHNLKYFTWIEQQKKSLD
                     ELNSQWDDFPRFWARIHLQMEEIDQRVEEFNERTGVLANL"
     gene            complement(1716..2978)
                     /locus_tag="VEL68_12045"
     CDS             complement(1716..2978)
                     /locus_tag="VEL68_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HXZ36742.1"
                     /translation="MDSRKKVLNWIQQNRNTIVGFLQDLIRIPSVTGEEGPIQEFIAG
                     RLKEMGFFVDVFEPDLAALRKHPAFVEVSRGYEGRPNVVGVLKGAGGGKSLLFNGHVD
                     VIPAGAPESWQHGSWSGDLADGRIYGRGSSDMKAGLAAMTMAVKAIRDSGIRLRGDLI
                     LEYTVDEELSGNGTLACVMKGYKADGGICCETSSMRVQPGSIGRIWFEIKVKGKAAGI
                     QRRWEGVNAIEKGYRVTQAVSDFERVRVDRLSHPLYPDIRGAIPCMVGVFESGSYHSA
                     FPDSCLLKGSMATVPGEDSAAVKKEFVEFIRAKVADDSWLKNHPPEVIFTGYFAEPSA
                     IPADSPIVQAICRKFTEVTGQEPVISGREGAADIRFMNRYGETPTVIFGPGMTEQMHA
                     NNEWANIEDLIQATKILSLTIQDWCGIR"
     gene            complement(3027..4205)
                     /locus_tag="VEL68_12050"
     CDS             complement(3027..4205)
                     /locus_tag="VEL68_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HXZ36743.1"
                     /translation="MKKLETLFAGVKLRNPLILASATPSWDGERCHQAWLHQAGAIVP
                     KTFAPPDKFAQHPRCGRMKIIRQGNTRIGMINIELYTTITLEEWLEKDLGRAHQGGSC
                     IIASVVAHPDPEATAANARTIQNTGKVAMFEINVSCPMPADQSCVGFLMGNDPESCYR
                     QVKALKKSLSLPVGIKLTPTTHNMVPMAQAAERAGADFITIGNSVRSFAGVDIKTGLP
                     KLPAYGGYSGPAIKPITQRHVSEVAKVVKTPISACGGVLTWEDVVEYIMLGATTVQTC
                     TSIMWRGYGHFQTLLKGLEGYMEAEKIESLDAIRGKALPYITSIEEVAKRPPLVSRVD
                     PETCINLKKGGCELCGPVCFYGAIEFSPRLKLKPKNCDGCNLCVEICPVGALSLAPPK
                     "
     gene            4679..4948
                     /locus_tag="VEL68_12055"
     CDS             4679..4948
                     /locus_tag="VEL68_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36744.1"
                     /translation="MTRIVHRADGCYGCKTCQLVCSLHHTGCFWPERSSIQVFRNPQT
                     GVVRWSIDETCDRCGREEPPLCVKYCSYQALHKVTNPDQEERRRE"
     gene            4941..6875
                     /locus_tag="VEL68_12060"
     CDS             4941..6875
                     /locus_tag="VEL68_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ36745.1"
                     /translation="MSKDGKIRGGLAGKILRVDLSTGKTSTEESGAYAERTLGGRGTN
                     SLIMVNEIEPGTGWSDPGNLLCFGTGSLVGTAAPGACRTDISTINVFSGGKGSANVGG
                     SWGAELKYAGFDHIVIRGKADRPVYLFVRDERVEIRDAAWLWGKTTYETENLLRQELG
                     DERVEIAAIGPAGENRVRGSAILIDTAKAAGGSGVGCVMGDKKLKAIVVRGHGKIEIF
                     DPRGFMEAVATAYRQCEANRVKVQSMRQSGAARFENLDFEGWETCMVVRNGQDDYWDR
                     EKRAQLMNPEAGIPSMRRKVRACFSCPTGCMPFMEKAQGKYREMKGEGFWINALWSVA
                     RFDISDPETAVAFWLLANELGLDTDYVAAGLAWVFECYEKGFIGLKDTDGLELTWGNG
                     EALIQLMHKLAHREGIGNLLADGMVEAAKKIGQGSEYYLIHLKGQPSIEPFRIPKGWG
                     LGVSTSPVAGRHLRGAIRGPVHSGPGGTDYDITHYPNQAKAVVWQAKTKELEDNLGIC
                     NYVGTWSGAHFLTLGNFAELVRTAMGLPVTEEDLMERYAVIGRNLEKAFNTLHTNLSR
                     EDDLPPQRFRREEVKTGSYKGLKADEDQYDAMLDEFYELWGWDKKSGMQNRESLEKVG
                     LKDVADKLAGKGLIEKHDKR"
     gene            complement(7035..8630)
                     /locus_tag="VEL68_12065"
     CDS             complement(7035..8630)
                     /locus_tag="VEL68_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ36746.1"
                     /translation="MVDLHLLFQGFGAVLSGYNIVALVLGSFFGIIIGAIPGLTATMG
                     IALLVPFTFGMSPFTGIVMLLGIYTGAIYGGGIASILIRTPGTPAAAATVFDGYPLAQ
                     KGMAGKAIGMATIASGIGGTFTALCLAFFAPILANFALRFSAPEYFALAVFGLSVTVT
                     LTGKSPLKGIISGCLGLLIAMIGLDPMGGFPRFTFGTTQLTGGFSFVPMLIGLFALSE
                     GFRQVEVILTAPKVSAVLTNIVPKLREIKECTRTFVRSCVIGLIVGVTPAIGAETACF
                     VGYSEAKRTSKHPELFGTGILEGVAAPQCAENASTGGDILPMITLGLPGDAATAVLMG
                     ALTIHNLQPGPLLFRDHLDLVHQIFAGMIMANIIFVTLGLTFARFFAKVINLDRRFLV
                     PLIFIACMVGSYAIDNDIFDTITCVFFGFIGYIMIRYNYPVSPMVLAQILGVMMESNF
                     RRSMAMSQDHLSILITRPIAVTILILAAFTTITGLRRMRKSAEMEARIAEEAAAARER
                     AQVVTRPPGENFPGKKANEVRDI"
     gene            complement(8654..9154)
                     /locus_tag="VEL68_12070"
     CDS             complement(8654..9154)
                     /locus_tag="VEL68_12070"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ36747.1"
                     /translation="MANLTMKKPDIIAVIIVIPICLYVFYESTKWPIPALLGRPFVIP
                     RGVATFLLIAAAILLYRAVRGRALPLENPLAGADLRRVSLTAVLTFAYLPVLERIGFI
                     GTTFLYMLLFAWVLGEHRWPRLILFSILVPFIIFAIFSTALHVPLPRGWIENALRTHG
                     FAAIFR"
     gene            complement(9229..10200)
                     /locus_tag="VEL68_12075"
     CDS             complement(9229..10200)
                     /locus_tag="VEL68_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36748.1"
                     /translation="MMRKYSWVLAGVFALALVFPSGAPAAGFPERDITLVVPWAAGGG
                     TDTLARTLAKNAKKYFGVNINVVNKVGGTGAVGMNSVATGRADGYTVGVITFNLSTYR
                     MIGLAELSYKDFDLIALLNRSPAGFSVKTDSQFKNLKELVEYAKANPGVVTVGHSGPG
                     QPWHLSAALLASSYKLKFTFVPFDGAAPTRTALVGGHITMASSGMDEVLQFYKTKQVR
                     ILAAITPTRNPFFPDVPTIGEAGYPIENPIFDWRGLGVAKGSPPEVLKVLREGFRKAA
                     EDPEYIKLMDELTLPRAYMEAEQFEQFLANLEKSLAPVLDTVGLLKK"
     gene            complement(10570..11526)
                     /locus_tag="VEL68_12080"
     CDS             complement(10570..11526)
                     /locus_tag="VEL68_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HXZ36749.1"
                     /translation="MTLAISRPYRQKEKGIHRGPVKFWALLLIPCLSVLLWIPRASGN
                     WSPLIDRLISDGFNEQVILGLFSRAEVQFDPAPMSSKLETLLKNQADNATPNSGYNSR
                     KVYRSLLKERTLARARSYLQEHREILEKISSEYCVPEEITVSILLVETRLGDLMGGRL
                     AFNSLASMALCTDLETIQPHLPKKLIHPKNEEFAREICWQKAHWAYSELKALILYAHF
                     NGYDPLNLPGSIYGAIGLCQFMPTSALIYGVDADRDGRIDLFMKADAFYSIANYLREH
                     GWSCRMDRASKHKAVYTYNHSDVYANTVLAVAEKLKDKSKKK"
BASE COUNT         2660 a         3361 c         3527 g         2455 t
ORIGIN      
        1 tatactttta taaataccaa ctgattctct cttcgccttg agtgggttaa atagcacgcc
       61 ggccacggag aaaccagcgc ctgttttggc ggcgtgaatt tgccccttcc ccgggcatac
      121 tcggaccctc cgatttccgc ctggctaaat tttttcacgg tctcaagggg gcaggaagga
      181 tttaatgccc ctaaaattac aagtttgcaa gaacccccgt ccgttcgtta aactcctcga
      241 cccgttggtc gatttcctcc atctgcaggt gaatccgtgc ccagaagcgc gggaaatcat
      301 cccattggga attgagttcg tccagggatt tcttctgctg ttcgatccag gtgaagtatt
      361 tcaggttgtg aatccgccgg cggtccagaa tactcaactc cagcatgttg tcagttcctt
      421 cgcccagcaa atagcggtgg taatccgcgg cggcctggac ctccgtgtag gggccccggg
      481 caacctgcat ctcctcgacc cgcgtcccgt agaggtccat ggagtccgtg agcaccgtca
      541 ggactagatc ccttttttgc aaggcgtagt atttgacgaa cttgattgcc gagagaagat
      601 ttccgatccc ggaaatgccc agcagggaaa gtcgggggat gaaatcccgg gaaaccccgc
      661 ggggctcgag atattttctt cccaccggtt cgttgaacag ccggacaagg ctgacgcagg
      721 cctcgtcgtc gatggccgta accacatccg tgttgcggac gttgtggatc caggggatat
      781 gcttatcccc gatcccttcg atgcgatgat cgccgtagcc gttcaggagc atggtggggc
      841 attgcagggc ttcgctggcc acgatccggc tccccaggaa tcgctccttc aggtagtccc
      901 cggcggcgat ggtcccggcc gaacccgtct gggacacaaa gccggcgcag cggccccctt
      961 cccctagttc cgcggataaa acctcttcga gagcgcggcc ggtgacctgg taatgccaga
     1021 ggtgatttcc gtattcgtcg aattggttga aaatgaccac gttcccccgg gtctggcgca
     1081 gttcccagca tttgtcaaaa atctccttca cattgctctc gcatcccgga gtcgcgatca
     1141 cctccccggc taccgtggcc agccactgaa atcgttcccg gctcatccct tccggcagga
     1201 tggcaatcga ctcgcacccc agaagcgtgg cattgtaagc tcctccccgg cagaaattcc
     1261 ccgtggaggg ccacgccgcc ttctggccgg tggggtcgaa ttggcctgta accagccggg
     1321 ggacgagaca accataggtg gcgccgacct tgtgcgagcc ggtggggaac cacttgccga
     1381 ccagggcgat gatgcgggca tcgacgccgg tgagctccgg cgggaattcc aggtaattga
     1441 ccttcccgaa tccccccccc gacggcacgg gttcgttctt ccaggtgata cgaaagaggt
     1501 tgagcggatt tacatcccag aggccgatgc ttctcagtct atccttcact ttcccgggaa
     1561 tacattcggg atgaatctgc tcttggaacg taggaatcag gatcttccgt tcccgcgccc
     1621 gctggacagc ttgttccagt ttgcgctcat tcttgctcaa gtcaatcatg gaagagcttc
     1681 tttctccttc gaccggtcaa tgccttttta cccggtcagc ggatcccgca ccagtcctgg
     1741 atagtcaagg ataagatttt cgtggcctga atgaggtcct cgatattcgc ccactcgttg
     1801 ttggcgtgca tttgctcggt catgcccggc ccgaaaatga ccgtcggggt ttccccgtaa
     1861 cgattcatga aacggatgtc cgcggcaccc tcccggccgc tgataaccgg ttcttggccg
     1921 gtgacttcgg taaatttccg gcagatagcc tggacgatgg gactgtccgc ggggattgcc
     1981 gaaggctcgg cgaaatagcc ggtaaagatg acctccgggg gatggttctt cagccaggag
     2041 tcatcggcga cctttgcccg gatgaactcc acgaattctt ttttgaccgc agccgagtcc
     2101 tcccccggaa ccgtggccat ggaacctttc agcagacagc tgtccgggaa ggcactgtgg
     2161 taggagccgc tctcaaaaac ccccaccata cagggaatgg cgccgcggat gtcgggatac
     2221 agagggtggc tcagccggtc tacccgcacc cgctcgaaat cggagacggc ctgggtcacc
     2281 cggtagccct tttcaatagc gttgacccct tcccaccgcc tttggattcc ggccgccttg
     2341 cccttgacct tgatctcgaa ccagatgcgc ccgatggagc caggctggac ccgcatgctg
     2401 gaagtctcac agcagatccc gccgtcggcc ttgtaccctt tcatcacgca ggccaaggtg
     2461 ccgttccccg agagttcctc gtccaccgta tattccagga tcagatcccc ccgcaggcga
     2521 atgccggaat cccggatggc cttgaccgcc atagtcatgg ctgccaggcc tgccttcatg
     2581 tccgaggacc ccctcccgta gatgcggcca tccgcaaggt cgccggacca actgccgtgc
     2641 tgccagctct ccggcgcacc cgccgggatc acgtccacat ggccgttgaa caggagggat
     2701 ttcccgccgc ccgctccctt gaggaccccc accacattgg gacgcccctc gtaaccccgg
     2761 gatacttcca cgaaggccgg gtgcttccgc agggcggcca ggtccggctc gaagacgtcc
     2821 acgaagaagc ccatctcctt gagccggccg gcgatgaatt cttggatcgg cccttcctcg
     2881 ccggttacgc tgggaatccg gatcaggtct tggaggaagc cgacgatggt gtttcgattt
     2941 tgctgaatcc agttaagcac ctttttgcga gaatccattt tatctcttcc ctttcgcctc
     3001 ttcccccgtt tcgccctttc gcccatttat ttcgggggcg ccagactcag cgctccgacc
     3061 gggcagatct ccacgcacag gttgcacccg tcacaattct tcggtttcaa cttcagccgg
     3121 ggagaaaact cgatcgcccc gtagaagcag accggcccgc agagttcaca cccccccttt
     3181 ttcaaattga tgcatgtttc ggggtccacg cgggagacca ggggcggacg cttggcaact
     3241 tcctcgatgc tcgtgatata gggaagcgcc ttgccccgaa tggcgtcaag ggattcgatc
     3301 ttttccgctt ccatgtaccc ctccaggcct ttcagcaaag tctgaaaatg cccgtacccc
     3361 cgccacatga tggacgtgca ggtctggacg gtggtggccc ccagcatgat gtattcgacc
     3421 acatcctccc aggtgaggac gccgccgcag gcggagatgg gggtcttaac gactttggcc
     3481 acttcggaga catggcgctg ggtaatgggc ttgatcgccg gcccgctgta gccgccgtag
     3541 gccggaagtt tgggaaggcc ggtcttgatg tccaccccgg cgaaggaccg gaccgagttc
     3601 ccgatggtga tgaaatcggc tcccgcccgc tctgccgcct gggccatggg aaccatgttg
     3661 tgcgtcgtcg gggtcagctt gatgcccacg ggaagggaaa ggctcttttt tagggccttc
     3721 acctggcggt agcagctctc cggatcgttg cccatgagaa agccgacgca ggactggtcg
     3781 gccggcatgg ggcaggaaac gttgatctcg aacatggcca ccttacccgt attctgaatc
     3841 gtccgggcat tggccgcggt cgcttccggg tccgggtgcg ccacgacgct ggcgatgata
     3901 cagctccccc cctgatgggc tctccccagg tctttctcga gccattcctc cagggttatg
     3961 gtggtgtaga gctcaatgtt gatcatgccg atccgggtgt tgccctggcg gatgatcttc
     4021 atccgcccgc accgcggatg ctgggcgaac ttgtcgggag gggcaaaggt cttcgggacg
     4081 atggccccgg cctggtggag ccaggcctgg tgacatcgct ccccgtccca gctgggggtg
     4141 gcagaagcca ggatcagggg attgcgcaac tttacgccgg caaaaagtgt ttctaatttt
     4201 ttcatggccg ctcctccatc ccgccttttc tcaccggccc gcgacgaaag gtagggatcc
     4261 cccgtcgggt ctctctccta gatcggccgt catcgttggt gggcttctgg gaaaggcttt
     4321 gggttttgct catcggaaac ggaaagaacg aattcagtat aacagaggaa ctcctaagcg
     4381 gcaaaattat tctgttcttt ttttgccagc ctcacccttt tccgtcacat tggcgatatc
     4441 cttggcatga gacagcccta ataccttaac tctcatcaac tctcatctga tgtagcgaaa
     4501 ggaaatgagc aaggctttct acctcattga caggcatccc tatctgtatt aacaagggga
     4561 aaagggggaa acccaagtcc gccatttatg aaatcgtgcc aagggcggcg aatcattggg
     4621 cagaaggaaa tttgaatcct aagctggaaa cgtactcaac tttcgagaaa gcagtcctat
     4681 gacccgaatc gtccatcgtg cagacggctg ctacgggtgc aaaacctgcc agttggtctg
     4741 ctcccttcat cacaccggct gcttctggcc ggagcggagc agcatccagg tattccgcaa
     4801 tccccagaca ggggtcgtcc gctggagcat cgacgaaacc tgcgaccggt gcgggcgtga
     4861 agaaccgccc ctttgcgtga aatactgttc ctaccaagcc cttcataagg tcaccaaccc
     4921 agatcaagag gaacgaaggc gtgagtaaag acggaaagat cagaggagga ctggcgggga
     4981 aaatcctgcg ggtcgatctg tcgacgggga aaacctcgac cgaggagagc ggggcgtatg
     5041 cggaacggac cctggggggg agggggacca attccctcat catggtcaat gagatcgagc
     5101 ccgggaccgg gtggagtgat ccgggaaacc ttctctgctt cggcacaggc tctctggttg
     5161 ggaccgcggc cccgggggcc tgccgcacgg acatctccac catcaacgtc ttcagcggcg
     5221 gcaaaggctc cgcgaatgtg ggcgggtcct ggggcgccga gctgaaatat gccgggttcg
     5281 accacatcgt catccgcgga aaagccgata ggccggtcta tcttttcgtt cgggacgagc
     5341 gggtcgagat tcgcgatgcc gcctggctct gggggaaaac gacctacgaa acggagaacc
     5401 tgttgcggca ggaactcggc gatgaacgag tcgagattgc ggccatcggc cctgctgggg
     5461 agaaccgtgt ccggggttcg gcgattctga tcgacacagc caaggcggcg ggagggtcgg
     5521 gcgtcggctg tgtcatgggg gataaaaaac tcaaggcgat cgtggtccgg ggacacggaa
     5581 agatcgagat tttcgacccg cgcgggttta tggaggcggt ggctacagct tatcgccaat
     5641 gtgaagccaa tcgggtcaaa gtccagtcca tgcgccagtc cggggcggcc cgttttgaga
     5701 acctcgactt cgaaggttgg gaaacctgca tggtggtccg aaacgggcag gacgactact
     5761 gggaccggga aaagagggcc cagttgatga acccggaagc ggggatcccg agcatgcggc
     5821 ggaaagtccg ggcctgcttc tcctgcccca ccggctgcat gccttttatg gagaaagccc
     5881 agggaaaata ccgggagatg aaaggggagg ggttctggat caatgccctc tggagcgtgg
     5941 cccgattcga tatttcggac ccggaaactg ccgtggcctt ctggctcctg gccaacgaat
     6001 tggggttaga cacggattac gtggccgcag gcctggcctg ggtcttcgag tgctatgaga
     6061 aagggttcat tggcctgaag gacaccgatg gcttggaact cacctgggga aatggcgaag
     6121 ccctgatcca gctgatgcac aaactcgccc atcgggaggg gatcggaaat ctgctggccg
     6181 acggaatggt ggaagccgcg aaaaaaatag ggcagggctc cgaatactat ctcattcacc
     6241 taaaaggcca gccctccatc gaaccctttc ggatccccaa aggatggggc ttgggtgtct
     6301 ctacatcgcc ggtagctgga agacacctgc gaggagcgat cagggggccc gtccactccg
     6361 gaccgggagg caccgactac gatattaccc attacccaaa ccaggccaag gcggtcgtgt
     6421 ggcaggccaa aacaaaggaa ctggaagaca acctcgggat ctgcaattac gtgggaacct
     6481 ggtccggggc gcacttcctg accctgggga acttcgccga actagttcgc acagcaatgg
     6541 ggcttccggt cacggaagag gacctgatgg aacgctatgc ggtgatcggc aggaacctgg
     6601 aaaaggcatt taacaccctg cacacaaatc tttcccgtga agacgacctt cctccccaaa
     6661 ggttccggcg ggaggaggtt aaaacgggat cctataaagg tttgaaagcc gacgaagatc
     6721 aatacgatgc catgctcgat gagttctacg agctctgggg gtgggacaaa aagagcggaa
     6781 tgcagaaccg cgaaagcctg gaaaaagtcg ggttaaagga tgtggccgat aaactcgcag
     6841 gaaagggatt aattgaaaaa cacgataaac gatagcttcg tttgaaccct cctattttcc
     6901 agtgtcccta ttcaaacctt tttactgaac tgctttaata tcggaatcaa cggacctacg
     6961 tccctgcctt ctctgcccgt gcctcgacga cgcggtcatc tacgacggct ccgattcggc
     7021 tgcggccgta gagttcaaat atcacgaact tcattcgctt tttttcccgg aaaattttca
     7081 cctggcggac gggttacgac ctgggcccgc tcccgtgcgg cagccgcttc ttcagcgatc
     7141 ctggcttcca tttccgccga tttgcgcatt cgtcgaagcc ccgtaatcgt ggtgaatgcc
     7201 gccaaaatca ggatggtcac cgcgatcggt cgggtgatta agatcgagag gtgatcttgc
     7261 gacatggcca tggagcggcg gaagttcgat tccatcatga cgcccaggat ctgggccaga
     7321 accatgggag agaccggata gttgtaccgg atcatgatat acccaatgaa cccgaaaaac
     7381 acgcaggtga tcgtatcgaa gatatcatta tcaatggcat atgagccaac catacaggcg
     7441 atgaaaatca gggggaccag gaagcggcga tccaggttaa tgaccttggc gaaaaaccgg
     7501 gcgaacgtca gcccgagggt cacgaagatg atgtttgcca taatcatgcc ggcaaatatc
     7561 tgatggacta ggtcgaggtg atcccggaag agcaaggggc cgggctggag gttgtggatc
     7621 gtgagagcgc ccatgagaac cgcggttgcc gcatcacccg gcaatcctag cgtgatcatg
     7681 ggcaggatgt ccccaccggt gctggcgttt tccgcgcact ggggcgcggc aaccccctcc
     7741 aggatgcctg tgccgaacag ctcaggatgt tttgaagtcc gcttggcttc gctgtacccc
     7801 acgaagcagg cggtctcggc gccgatggct ggcgtcaccc ccacgatcag gccgataaca
     7861 caggagcgca cgaaggtccg tgtgcactcc ttgatctctc gcaatttagg gacgatgttc
     7921 gtcaggaccg cgctgacttt gggcgcggta agaatgactt caacctgacg gaagccctcg
     7981 gaaagggcga agagcccgat cagcatcggg acgaaggaga acccgccggt cagttgagtt
     8041 gtgccgaagg taaaccgcgg gaatcccccc ataggatcca agccaatcat ggcgatgagg
     8101 agccccaggc accctgagat gatgcccttc aacggggact tgccggtgag cgtgaccgtg
     8161 accgagagcc caaagacagc cagggcgaag tattcggggg cggaaaagcg cagggcgaag
     8221 ttggcgagga tcggagcgaa gaatgcgaga cagagcgcag tgaaggtccc gccgatccca
     8281 gaggcgatcg tggccatgcc gatggctttt ccggccatcc ccttttgggc caagggatac
     8341 ccgtcgaaca cggtcgccgc tgccgccggc gttcccggtg tcctgatcag gatggaagct
     8401 ataccgccgc cgtagatggc ccccgtatag atgcccagga gcatgacaat cccggtgaag
     8461 gggctcatgc caaacgtgaa cggaaccagg agggcgatcc ccatggtcgc cgtgagcccg
     8521 ggaatggctc cgatgataat gccgaagaac gagcccagga ccagcgccac aatattgtag
     8581 cctgagagaa ctgccccgaa gccttggaac agtaaatgaa ggtcaaccat gaaagggaac
     8641 cctcagagga atgctaccgg aaaatggccg cgaagccatg tgtgcgcaag gcgttctcga
     8701 tccatccgcg cggcagtggg acgtgcaggg cggttgagaa aatcgcaaat atgatgaatg
     8761 ggacgagaat cgaaaaaaga atcaggcgtg gccaacgatg ctcgcccagc acccaggcga
     8821 agagtagcat gtacagaaat gtcgtgccga tgaatccaat gcgctcgagc acggggagat
     8881 atgcgaacgt gagcacggca gtcaggctca cccggcggag gtccgcgcct gccaatgggt
     8941 tctccagggg gagcgcccga ccccttacgg cacggtacag gaggatggcc gcggcgatca
     9001 gcaggaaggt cgcgaccccg cgtggaatca caaacggccg gccgagcagc gcagggatgg
     9061 gccacttcgt ggactcatag aatacgtaga ggcagatcgg aatgacgatt atgacggcga
     9121 tgatatccgg cttcttcata gtcaaattgg ccatggaagg ctccgatatg cgctgaggtt
     9181 gacgatggac ggaagggcga tcgccctccc gcccgtcgca agagagcctt acttcttcag
     9241 cagaccaacc gtatccagaa cgggtgcgag acttttctcg aggttggcca ggaattgctc
     9301 gaactgctcg gcctccatgt aggcccgggg aagggttagc tcatccatca gcttgatgta
     9361 ctccgggtcc tctgctgcct tgcggaaccc ttcgcggagc actttcaaga cctccggcgg
     9421 cgagcccttg gccacgccca gtccgcgcca gtcgaaaatc gggttctcga tcggatagcc
     9481 ggcctcgcca attgtcggga cgtcggggaa gaatggattg cgggtcggag tgatggccgc
     9541 aaggatcctg acttgcttcg ttttgtagaa ctggagtacc tcatccatac cagacgaggc
     9601 catcgtgatg tgtcccccga ccagcgcggt acgcgtcggg gccgcgccgt caaaggggac
     9661 gaacgtgaac ttcagtttat aactcgaagc cagcagagcc gcggaaagat gccagggctg
     9721 gcccgggccc gaatgaccca cggtcaccac accgggattg gcttttgcgt actccaccag
     9781 ttccttcaaa ttcttgaact gggaatcggt tttcaccgag aagcctgccg gactccggtt
     9841 gaggagggca atcaggtcaa agtccttgta ggagagttcg gcaagaccga tcatccgata
     9901 cgtgctgaga ttgaatgtga tcacgccgac ggtgtaaccg tccgcccggc ccgtcgccac
     9961 ggagttcatg ccgacggcac cggtgccgcc gaccttgttc accacgttga tgttgacgcc
    10021 aaaatacttc ttggcattct tggcaagagt tcgggccagc gtgtcggtcc cgccgcctgc
    10081 tgcccacggt acaaccaggg taatgtcccg ttccgggaat ccggccgccg gcgccccgga
    10141 aggaaacacc agagccaggg cgaagacccc cgccaatacc caactgtact tgcgcatcat
    10201 gacagcctcc tttcgatgtg ctcgccggca cacccttagc gataaaaaga attaaggaac
    10261 agaaaggcct ttcgatgaaa ggattatatg ccaaggaaac tggagtgtca aaggggactt
    10321 cttaaatgcg gaatcaaaga cggaggaata gaggagatgt ggaatccgga acgccgattt
    10381 cggaatggaa gacaagggaa actaatccac tgaggaaggc agactgaaag ggtctcgccc
    10441 ctggggggat cgagatcaaa aggatttaat cgcgggcttc agaaaaacaa tcggaaggag
    10501 attaataaag gagctggaat attgctttcg gcatgtagaa tttctatctg cgttcttata
    10561 cgtcccattc tatttctttt tggatttatc ttttagcttt tcggccacag ccaggacggt
    10621 gttggcgtaa acgtcactgt gattataggt gtaaaccgct ttgtgcttgg aggcccggtc
    10681 catccggcag ctccaaccgt gttcgcgaag gtaattggcg atgctgtaga aggcatcggc
    10741 cttcatgaat agatcgatgc gaccgtctcg gtcagcatcg accccgtaaa tgagggcgct
    10801 cgtgggcatg aactggcaaa ggccgatggc cccgtagatg gagccgggaa ggttcagggg
    10861 gtcatatcca ttaaaatggg cgtacaggat gagagccttg agctcggagt aggcccagtg
    10921 ggccttctgc cagcaaatct ccctggcgaa ttcttcattt ttcggatgga tcaatttttt
    10981 cggaaggtga ggctgtatcg tttccaggtc ggtacacaga gccatgctgg ccagagaatt
    11041 gaaggccaat cttcccccca taagatcacc tagacgggtt tccaccagaa ggatggagac
    11101 ggtgatctcc tcgggcacgc agtattcgct gctgattttt tcaaggatct cccgatgctc
    11161 ttgaaggtaa gaacgggcgc gagccagggt tctttcttta agaaggctcc ggtagacctt
    11221 cctggagtta tatcctgaat taggggtagc attatcggct tggttcttaa gaagggtttc
    11281 cagcttgctc gacatggggg ctggatcgaa ctggacctct gccctcgaga agagacctag
    11341 aatgacctgc tcattgaagc cgtcggaaat gaggcggtca atgaggggag accaattccc
    11401 gcttgcccgc ggaatccaaa gcaggacgga gaggcagggg ataaggagaa gagcccagaa
    11461 tttaacgggg cctcggtgaa tccctttttc tttttgccga tagggtctgc tgatcgccaa
    11521 tgtcaaatga aaaattggtt ccgcttataa gattgaaaaa agaccattaa gaatatgcga
    11581 aaaatgtgcc aaacgaagag aagtgccgcc gagatgaaag ccgttaaaac ttttgggtct
    11641 ccagaaatac ctctattccc tcagaaggat ttataatcaa atgatttcgc tcggaatggg
    11701 gagagttcag gagcaagagg aaagaggaag gagggaattg acgaccaaac cagcggtttc
    11761 ctcgcagcac cccggagaca aaagggaaat aactacaggg taggatggaa tgatctccct
    11821 agacttttct ccccagctga agtaaaacgg tcccaaaact gcccccaatc ctttttctcc
    11881 cttcttcagg gagaggagct tcagtagtga aactcggcga tgacgacgtg cccctcctcg
    11941 agccgatagg tgagcaccat aagggtttcc ttgttcaata ctaccttaac cggtcgtacg
    12001 ggc
//
LOCUS       DASPRR010000325         5383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1040025, whole genome shotgun sequence.
ACCESSION   DASPRR010000325 DASPRR010000000
VERSION     DASPRR010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040025"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VEL68_12085"
     CDS             <1..405
                     /locus_tag="VEL68_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HXZ36750.1"
                     /translation="DGIYEKVKSDMIIFSGVELTVFAKGYIHVSRVNGEKEVLHILNH
                     LGDYNLSREQMMDRIRTLGGMFPLDTVEITSKGFRCQEWEIPEIPYPKLAADDSHSRE
                     GIGRAWVELEAKRNKDSILRAIKRGDFWNCYL"
     gene            479..1624
                     /locus_tag="VEL68_12090"
     CDS             479..1624
                     /locus_tag="VEL68_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ36751.1"
                     /translation="MSRRVAICAVAQIKNEPDIWYKRFQGMVLDCVEQILKQTGVTFD
                     LKKGIRNVVTCSDDVFDARTISDNALTDVVGAHYRGEEKMAQDGINGLGYSMACILSG
                     HDEVVLLAGHCKESQSESRRMCTNLAFDPFYCRPLGMDFTNVNALQARAYQAKSGIQE
                     EHLAKIVVRSRKNGVRNPFARENRTVTETEVMKSPMLADPVRELHHYPATDWAVAFLL
                     SNEERAKEFTDRPVWISGFANCMDTYFLGDKDLASNFSLKQAAGKAYQMAGIKDPRKE
                     INLFELNDAVAYQLPMWAEGVGLCEEGKGGRWIDEGWMEKHRVNLSGGMLNGNPVMIG
                     GLARAAEAVIQLRGEAGSRQEDGVRRALAHGTTGAAGQHQAVIILEK"
     gene            1721..2920
                     /locus_tag="VEL68_12095"
     CDS             1721..2920
                     /locus_tag="VEL68_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ36752.1"
                     /translation="MAQGKKNRNVGIIGIGQTHYSSHREDVNQPEMIQEAVRLALTDA
                     GISIQDVDCVVHGNMELFEMIHQPDLWHVLGTGAYGKDSFRITTGGTTGSTLVAAADN
                     LAASGMYNVVLAIGFEKLQEGHTTGGITNMADALWAKNLQTGALTGHTAARMIKEFGE
                     DRAKRAAMTLRIIMDKHACLNPNSHRAFGLEFGQMESLMETSPKLVGDLRLIHMCSQS
                     DGACALILAEEKRAKEISKKPVWIRDHITIHREESFNLFGLEPVEMTHRFAARELFAR
                     NGIQNPLEYFDVFEMYDPSAWWGLEWLRDFFLLEGDEHLKLVENKEVMIGGKIPMNPS
                     GGVVAANPIGATAMVRVAEAALQVRGDAGRHQIPKPVKHALASGFGGTMWTVLFLLEK
                     ELTGWGE"
     gene            2922..4103
                     /locus_tag="VEL68_12100"
     CDS             2922..4103
                     /locus_tag="VEL68_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HXZ36753.1"
                     /translation="MSEYFGVKVDDLFNTMPDRFRPEGAEGVDASFGYDIAGEGKWRL
                     TVKDRKMKIEKTPDLSGCVTVTTTDGETFVGINLGKVDAMKAFSSGKFKVEGDFGALA
                     KTGKMFRKYVPPKRELTPKEYILDMFGTLEKRFQPARAEGLDVSIGYDIGGQDGGRWT
                     AVIQGGKCVVKEGLAENLSVTLQAQAPDWVALILGKIDALSLLSAGKAAIAGDAKLAL
                     RLGEIFSPYAPPGEKAAEQELIVLRKTISVNQKFATGPVMGKFLKALKEKKIMANQCP
                     RCGRLQLPPREVCAPCRVRATEWKEVGPKGHLMVMDVVYYASPDPLTGETRETPYGSI
                     HVLLDGCKGHETFWHLLRRDQIFRVKEAWGEERGTRLRPVWAEHRTGNIHDIQYFEID
                     E"
     gene            4195..4656
                     /locus_tag="VEL68_12105"
     CDS             4195..4656
                     /locus_tag="VEL68_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ36754.1"
                     /translation="MPEDFDRSDCFTVEGKLALPYTYFAGKMGSRFITTIRDQKKILG
                     VKCKTCNKVFIPPRQTCALCMEDIRENWVELKNEGEVTNYTVVRYNDKHLPRKPPFIL
                     AMIKLREADTPMVHILEGIQPEKVKIGLKVKPIFAAQTTNTILDIDHFEPL"
     gene            4792..>5383
                     /locus_tag="VEL68_12110"
     CDS             4792..>5383
                     /locus_tag="VEL68_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HXZ36755.1"
                     /translation="MKEILADLKAEQESLDQFLSTLAEPQWDFPTPAEGWTVRDSVSH
                     IAHIDEVAVAFIRGDTSALEEVKKFGLKYTTVKAQKGREMKVSEILPWWRRARNVLLD
                     ELSKCDPKQRIPWFAIPMGARAFATARLMETWAHGLDCFDALGVEPEDTDRLRHVALM
                     AYMARTYAYQVNGLSLPQTPLRLELMLPSGHLWTYGE"
BASE COUNT         1264 a         1423 c         1645 g         1051 t
ORIGIN      
        1 gatggaattt atgaaaaggt gaagtcggac atgatcatct tttccggcgt tgaattgacg
       61 gtctttgcaa aaggatacat ccatgtgagc cgggtaaacg gagagaagga ggtcctccac
      121 attctgaatc atctgggaga ctacaacctg tcccgcgagc agatgatgga ccggattcgg
      181 accctgggcg ggatgtttcc tctggacacg gtggagatca cctcgaaagg gttccgctgc
      241 caggagtggg aaattcccga gatcccctac cccaagcttg ccgccgatga ttcccacagt
      301 cgggaaggaa tcggccgggc ctgggttgag ctggaagcca aacgaaacaa ggactcgatt
      361 ctgagagcga taaagagggg agatttctgg aactgctacc tgtaatttcg gatttcggat
      421 tgaagagccc gaaacagaag gctttttgat ccgcaatccg caatcgaaag gggacgttat
      481 gtcgagaagg gttgcgatct gcgcggtggc ccagatcaag aacgagccgg acatctggta
      541 caagcgtttt cagggcatgg tcctggattg tgtagagcag attctgaagc agaccggagt
      601 gacctttgac ctgaagaagg gcatccgcaa tgtggtcacc tgctccgatg acgtcttcga
      661 cgcccggacg atctcggaca acgcattgac cgacgtggtg ggagcccatt accgggggga
      721 agagaagatg gcccaggatg ggatcaacgg attaggttac tccatggcct gcattctttc
      781 cgggcacgac gaggtagtcc tcctggccgg gcactgcaag gagtcgcagt ccgagagccg
      841 gcggatgtgc accaatttgg ctttcgatcc cttttattgc cgccccttgg ggatggactt
      901 caccaatgtc aatgctctcc aggccagggc ctaccaggcc aaatctggga tccaggagga
      961 acacctggcc aagattgtcg ttcgctcccg aaaaaacggg gtccggaacc ccttcgcccg
     1021 ggagaatagg acggtgaccg agacagaagt gatgaaatcc cccatgctgg ccgatccggt
     1081 gcgggagctg catcactacc cagcgaccga ctgggcggtt gcctttctcc tgagcaatga
     1141 agaaagagct aaggaattca cggacaggcc cgtttggatc agcggatttg ccaattgtat
     1201 ggatacctat ttcctgggag acaaggacct ggcttccaat ttctccctga aacaagcggc
     1261 cgggaaggcc taccaaatgg ccggcatcaa agatcccaga aaagagatca acctgtttga
     1321 gttgaacgac gccgtagcct accagcttcc catgtgggcg gagggagtcg ggctctgcga
     1381 agaaggaaag ggaggccgct ggatcgatga gggctggatg gaaaaacacc gcgtgaacct
     1441 gtccgggggg atgctgaacg gaaacccagt catgatcgga ggattggcgc gggccgccga
     1501 agccgtcatc cagctccgag gcgaagcagg atcgcgtcag gaggacggag tccggagggc
     1561 cttggcacac ggtaccacag gggcagccgg gcagcaccag gcggtgatca ttctggaaaa
     1621 atgataaaca agagagttcc gggtttcaag tttcaagttc aggccatttt tgattctgca
     1681 ttctgaaatt tgtaatccga attttaattg ggaggcatcg atggcgcagg ggaagaagaa
     1741 tcgcaatgtg gggatcatag gcatcgggca gacccactac tccagccacc gggaagacgt
     1801 gaaccagccg gagatgatcc aggaggcggt ccggttggcc ctgacggatg ccgggatctc
     1861 tatccaggat gtggactgcg tggtccatgg gaatatggaa cttttcgaga tgattcacca
     1921 gccggatttg tggcatgtgc tggggaccgg tgcctacggc aaagattcct tccgcatcac
     1981 cacgggggga accacgggtt caaccctggt ggctgccgcg gataacctgg cggcttcggg
     2041 catgtacaat gtggtcctgg ccatcgggtt cgaaaagctt caggaggggc acaccacggg
     2101 ggggatcacc aacatggccg acgccctgtg ggccaagaac ctgcaaactg gcgctctaac
     2161 cggccatacg gccgcgagga tgatcaaaga gttcggggag gaccgggcca agcgcgcggc
     2221 catgaccctg cggatcatca tggataaaca cgcctgcctg aaccccaact ctcaccgcgc
     2281 tttcggtctc gaattcggcc agatggaaag cctgatggaa acctctccca agctggtcgg
     2341 ggacctgcgt ctgatccata tgtgctcaca atccgacggc gcctgcgccc tgatcctggc
     2401 ggaagagaag agggccaagg agatttccaa gaagccggtc tggattcggg accacatcac
     2461 gattcatcgg gaggagtctt tcaacctctt cgggctggaa ccggtggaga tgacccatcg
     2521 ctttgccgcg cgcgagctgt tcgccaggaa tggaatccag aatcctctgg aatattttga
     2581 cgtcttcgag atgtacgacc cttcggcctg gtgggggctg gagtggctgc gggacttttt
     2641 cctcctggaa ggggacgagc acctgaagct ggtggaaaac aaggaggtca tgatcggggg
     2701 gaaaattccc atgaacccgt ccggtggagt ggtcgccgcc aaccccatcg gggccacagc
     2761 catggtccgg gtcgccgagg cggcgctgca ggtccgggga gatgccggac gccaccagat
     2821 ccccaaacca gtgaaacacg ccctggcttc gggattcggc ggcaccatgt ggacggtcct
     2881 tttccttctg gaaaaagagc tgactggatg gggggaatga catgagcgaa tattttggag
     2941 tgaaagtgga cgacctcttc aataccatgc ccgaccgctt ccggccggag ggtgcggagg
     3001 gcgtcgatgc ttccttcggg tatgatatcg ccggggaggg gaaatggagg ctaacggtca
     3061 aagaccggaa gatgaagatc gagaagactc ccgacctgtc cggctgcgtg accgtcacca
     3121 ccaccgatgg ggagaccttt gtggggatca acctggggaa ggtggatgcc atgaaggcct
     3181 tcagttcggg caagttcaag gtggaagggg acttcggagc cctggccaaa acgggtaaga
     3241 tgttccggaa gtatgtgccc cccaaaagag aattgactcc caaggaatat atcctggata
     3301 tgttcggcac cctggagaag agattccagc cggccagggc cgaaggcctg gatgtctcca
     3361 tcggttatga tatcgggggg caggatggag ggaggtggac cgccgtcatc cagggtggaa
     3421 agtgcgtcgt caaggaaggc cttgccgaga acctgtccgt gacactccag gcccaggccc
     3481 cggactgggt tgcgctgatc ctgggaaaga tcgacgcctt gagtctgctg agcgcgggca
     3541 aggcggcgat cgcgggggat gccaagctgg ccctgagact gggggagatt ttctctccct
     3601 atgcccctcc cggtgaaaag gcggcggagc aggagctgat cgtcctaagg aagactatct
     3661 cggtgaatca gaaattcgcc actggcccgg tcatggggaa gtttctcaaa gcccttaaag
     3721 agaagaaaat catggccaac cagtgccctc ggtgcggccg gctgcagctt cctccccgag
     3781 aagtatgcgc cccctgccgg gtccgggcga cggaatggaa ggaagtggga ccgaagggcc
     3841 atctcatggt aatggacgtc gtttattatg ccagccctga ccccctgacc ggagagaccc
     3901 gggagactcc ttacggctcc atccatgtcc tcctcgacgg atgcaagggg cacgaaacct
     3961 tctggcacct gttgcggaga gaccaaatct ttcgggtgaa ggaagcctgg ggcgaggaac
     4021 ggggaacccg cctgcggccg gtttgggccg agcaccggac aggcaacatt cacgacattc
     4081 aatacttcga aatcgatgaa taagagattt aaagaagtct cgggtttcgg gcttcaagtt
     4141 tagacctaaa aatccaagag cccttaaccc gaattttccg ttctggaggg aaaaatgccc
     4201 gaagactttg atcgatccga ttgctttacc gtggaaggga aattagccct tccctacacc
     4261 tactttgccg gcaagatggg gtccaggttt ataaccacca ttcgggacca aaagaaaatc
     4321 ctgggggtga agtgcaaaac ctgcaacaag gtttttatcc ctccccggca gacctgcgct
     4381 ctttgtatgg aggacatacg agaaaactgg gttgaactga aaaacgaggg cgaggtgacc
     4441 aactacacgg tggtccgtta caatgacaaa catctgcccc gcaaacctcc ctttatcttg
     4501 gccatgatta aactcaggga ggccgatacc cctatggtcc acattctgga aggtatccaa
     4561 ccggagaagg tgaagatcgg cttgaaagtt aagccgatct ttgccgccca gacgaccaat
     4621 accatcctgg acatcgatca tttcgaaccc ctctgagccc agccggttcc cctccccctc
     4681 cgagtgagag ggggcggggg cggaaggagg ataacctata gggcaattca tgaactgccc
     4741 ctatgagaac tccatgaaga agaaggaatc acagcccggg agacttaccc catgaaagag
     4801 attctcgccg acctgaaggc tgagcaggaa tctttggatc agtttttgtc caccctggcc
     4861 gagccccagt gggactttcc cacccccgcg gaggggtgga cggtcagaga ctcggtatcc
     4921 cacatcgccc acatcgatga ggtggccgta gccttcatcc gcggagacac cagtgccctg
     4981 gaagaagtca agaaattcgg attgaagtat acgacggtga aagcccaaaa gggccgggag
     5041 atgaaagttt ctgaaatcct gccctggtgg cggcgtgcca ggaatgtcct gctggatgag
     5101 ctctccaaat gcgacccaaa gcagaggatt ccctggtttg ccattcccat gggagcccgc
     5161 gcctttgcca ccgcccgttt gatggaaacc tgggcccatg ggcttgactg ctttgatgcg
     5221 cttggagtgg aaccagaaga taccgaccgc ctccgtcatg tcgccctcat ggcctacatg
     5281 gcccgaacct atgcctacca ggttaatggt ctttccttgc cgcagactcc cttacgtttg
     5341 gagctcatgc tgccatccgg gcatttgtgg acttatggtg aac
//
LOCUS       DASPRR010000326         7914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1364754, whole genome shotgun sequence.
ACCESSION   DASPRR010000326 DASPRR010000000
VERSION     DASPRR010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364754"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VEL68_12115"
     CDS             <1..1087
                     /locus_tag="VEL68_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXZ36756.1"
                     /translation="PERVDLALDTTGMDRVLDFDPRNLNLEVSAGMTLDQINEFLAGQ
                     EKGFFLPLDPPGARRATIGGAYAANSSGPMRLRYGTLRDLVLGVRGIDSRGRDVAFGG
                     KTVKNVSGYDLTKFLIGSAGSLCLITSVATRVYPLPEASLLCDLIFETLEEVEKFLAA
                     LRSSVFIPSAVAMTELAGGPGVSDTAGIRFRVRIGFEGHRLAVERQNKDLPKLAGEFG
                     GFGDARSGRDGMIRGIRSAVDPDRPFENPLFLKITVPIAQGPRTFASIHKAAQQYGLP
                     AKLVLCAANGLIFLYIGEAHRNPAGELLRDLKDLISTAGGYLAPLLGHRSLFASWGAR
                     VEPALHQLAFQPIKERLDPTGVFPPIV"
     gene            1162..2421
                     /locus_tag="VEL68_12120"
     CDS             1162..2421
                     /locus_tag="VEL68_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ36757.1"
                     /translation="MDEALKAVYDEVSKCNRCGFCQAHCPIFEVTGDERNVARGHNVH
                     VRKLVEGSLEMNQDFKDPLFDCLLCRACVTNCFPAVRTDQNVIAGRAEYLKRVGQPKT
                     LRFLFRSLLPDHARLSSFVRLAAVSKNSGATRLARAMGVLNWFGRDLDKVEGIVEKLP
                     TKFFRERAASRPTAPKAGKIRVSYFVGCAFNFILPQASEATLEVLEKTGANVQIAENG
                     CCGSPAYEYGDLEAAREMARKNLEALEKTGVEAVVTDCGRCATFLKEYPQLLESDPAL
                     QEKARQVARKVKSFSEFMQPFIRESPPAKFLSGKVTYHDPCHLSRYQKVTQEPRALLK
                     RIPGLTYVELPEADRCCGGAGSYTISRYDQSMKVLDRKMELVRKTGADILATECPSCI
                     IQLSHGARRAGLGTRVVHLSELLREVI"
     gene            complement(2524..3174)
                     /locus_tag="VEL68_12125"
     CDS             complement(2524..3174)
                     /locus_tag="VEL68_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HXZ36758.1"
                     /translation="MITLVILVISLILLLLLLIHEKREEKNLRLITKTILSCMFVWVA
                     FRQSSSLPDYSFCLIVGLVLCLIGDVCLGWPGEKAFRAGLVAFLLGHIFYIIAFSRLI
                     GLSAWAVPTAILFWLFSLFAFLWLQPHLKAMLVPVGIYILVITSMVTGAWAVFAGTSL
                     SYGGRLFVLAGAFLFYLSDLFVARNQFIKGDFFNRLIGLPLYYLGQFLLAFSIGLF"
     gene            3893..4093
                     /locus_tag="VEL68_12130"
     CDS             3893..4093
                     /locus_tag="VEL68_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HXZ36759.1"
                     /translation="MISLTRLNNTGLVINPDLIVFMEETPDTIITLSNGEKIAVQEKI
                     KEVIRRVINFRRCIFNPSIPIE"
     gene            4098..4925
                     /locus_tag="VEL68_12135"
     CDS             4098..4925
                     /locus_tag="VEL68_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HXZ36760.1"
                     /translation="MDKATLIGLFLGFAAIIGGQVLEGGSLHSIMQFTAAVIVMGGTF
                     GAVFVSYPFQDVMEGFRGVRKVLKEPPQDPFQIIQQIITYANKARREGILSLEKEIKN
                     IRDPFLAKALTMSVDGIEPHVIREAMESELEYLNEYGKVSSKVWRAAGGYAPTIGILG
                     AVLGLIHVMENLSDPGKLGAGIAVAFVATVYGVGSANLLFLPMATKLEVRHRQVMILN
                     EMILEGVVALSTGENPRLIEEKLTSFLSDLQKEKRSTSVSKAPAGAAAGKTLQEESG"
     gene            4972..5787
                     /locus_tag="VEL68_12140"
     CDS             4972..5787
                     /locus_tag="VEL68_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HXZ36761.1"
                     /translation="MKKRKKHQEEEHENLERWLVSYADFITLLFAFFVTMYAISRVDE
                     RKLGSMVESLHRALGSTMLTQASHPEPGAFQSSAYPVNVAIVPTPREKGVSSEDFEKL
                     AQDIRKKMAERMEHDGSYGEASLNELKFIIDKRGLVLRFSERFFFDSGDASIRPEVVP
                     LLTVLAQSLEALPNQVRIEGHTDSVPIRTTRFPSNWELSTARATSIVHYFLTHHRFEP
                     NRLSAAGYGEFRPIAKNQTADGRSQNRRVDIVILSAKEEENEPGMEKKVYPTI"
     gene            complement(5802..6548)
                     /locus_tag="VEL68_12145"
     CDS             complement(5802..6548)
                     /locus_tag="VEL68_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXZ36762.1"
                     /translation="MATPNSHEEAILDPQAQKLLAAFIDSHRAATLGSLLKGIIHNLN
                     GSLQILSMQMELLQRMLPPGEDKVQEQMEKCLGQISRFKGMLELLIQKGIHDEQESPQ
                     SIQLNDILEEELSLLYHDLFFKHQVKVDKDLASSLPLLRGSYMDFSQALWNLIQNSLE
                     AMENSRRKELILRTRVQGRQVQVTIQDTGCGIPEEAKPRLFEPSFTTKGRKHAGLGLF
                     VARRLLAPYGASFHYSSKEGETVFEVSFPV"
     gene            complement(6754..7455)
                     /locus_tag="VEL68_12150"
     CDS             complement(6754..7455)
                     /locus_tag="VEL68_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ36763.1"
                     /translation="MDSEILPFRIWIADDDDDFRETLGKSLAQEKRSIRLFANGEDLI
                     EALKRDPSFDIIIADLLMPGGDGLQVLEKSKKINPDGVVVIMTGYASLDTAIQAIRGG
                     AYDYIRKPFKLDELEVIVKNAGDKIALIRENRRLLQKLKDAMEEMKGLKKPSPEFHPP
                     VESVPSLALDHKLTEMGVILNQLIPPDYDFKEREERGKAFQELQKLIEYKREGLLSET
                     EFTSLKRILFQSFKE"
     gene            complement(7519..>7914)
                     /locus_tag="VEL68_12155"
     CDS             complement(7519..>7914)
                     /locus_tag="VEL68_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36764.1"
                     /translation="VEALWEFVAISETLYQCLLTRDFGEIGRQLEKRENLVHSIDDID
                     RKILQASSGTSQAGPGGTPEADEDPAVHNLKALLREAADLNAQCLAQAALLPEEFKKE
                     LATTHEEWAAARWFLQQAGSAPRFMDIQR"
BASE COUNT         1890 a         2100 c         2208 g         1716 t
ORIGIN      
        1 gccggagagg gttgacctgg ctttagatac aaccggaatg gacagggttc tggactttga
       61 tccccgaaat ctcaatctgg aggtttcggc gggtatgacc ctggatcaga tcaacgagtt
      121 tctggccggc caggaaaagg gtttttttct tcccctggac ccgcctgggg cgcgacgggc
      181 gaccatcggg ggagcgtatg ccgccaactc cagtgggccc atgaggctcc gctacgggac
      241 ccttcgtgat ctcgtcctgg gggttcgggg aatcgattcc cgagggcggg atgtggcctt
      301 tgggggaaag acggtcaaga atgtttcggg ctatgatctt accaaattcc tcatcgggtc
      361 tgccggaagc ctctgcctga ttacttccgt tgcaacccgg gtgtaccctt tgccggaagc
      421 atctttgctc tgcgacctga tctttgaaac tttggaagag gtggaaaaat tcctggccgc
      481 cctccgctcc tcggttttca tcccctcggc ggttgcaatg accgaacttg ccggaggtcc
      541 cggtgtgtcc gatacggccg gaatccgctt ccgcgtaagg atcggttttg aaggccaccg
      601 cctggccgtg gagaggcaga acaaagacct tccgaagctt gccggagagt ttgggggatt
      661 cggggatgca aggagcggtc gggatggaat gatacgggga attcggtccg cggtggaccc
      721 cgatagacct tttgaaaatc cccttttctt gaaaattacc gttcccattg ctcaggggcc
      781 tcggactttc gcttctatcc ataaggcggc ccaacaatac ggacttccgg cgaaattggt
      841 tctctgtgcc gccaatgggc tcatcttcct ctatatagga gaagcccatc ggaatccggc
      901 cggggaactg ctccgggacc tcaaggacct gatttctacg gcgggcggtt acctggcccc
      961 tcttctgggt caccgaagcc ttttcgcgag ctggggagcc agagtggaac cagccttgca
     1021 ccagctggcg ttccagccta tcaaggagag actcgacccc actggtgttt ttccgccgat
     1081 cgtatgaaag aaagggttcc aggttcaggg gcgcaggttt caaggttgaa gccttaaaag
     1141 agttactaac cgaggaagga tatggatgag gcattaaagg ccgtttacga tgaagtctcc
     1201 aaatgcaacc ggtgcggttt ctgccaggcc cattgtccca tattcgaggt gacaggagac
     1261 gagcggaatg tggctcgcgg gcacaacgtg cacgtgcgca agctagtaga gggcagcctg
     1321 gagatgaacc aggacttcaa agatcccctc ttcgactgcc ttctgtgcag agcctgcgtg
     1381 accaactgtt ttcccgccgt gcgcacggat cagaacgtaa tcgccggacg ggcggaatat
     1441 ctgaagaggg tcgggcagcc caagaccctg cgcttcctct ttcgtagcct cctcccggat
     1501 catgcccgcc tctcctcctt cgttcggctg gccgccgtga gcaaaaactc cggagccacc
     1561 cggctcgcac gggctatggg tgtgctcaac tggtttggcc gggacctgga caaggttgag
     1621 ggaatcgtag aaaagcttcc caccaagttc tttcgggaga gggccgcttc ccgacccacc
     1681 gcacccaagg ccggaaagat tcgggtttcc tacttcgtgg gctgcgcttt caattttatc
     1741 ctcccccagg ccagcgaggc gaccttggag gtgctggaaa aaaccggggc gaacgtgcag
     1801 atcgcggaaa acggctgctg cggctctccg gcctatgaat acggagactt ggaggccgcc
     1861 cgggaaatgg cccgaaagaa cctggaagct ctggaaaaga caggggtcga agcagtggtg
     1921 accgattgtg ggcggtgcgc gacttttctc aaagaatatc cccaactgct ggaaagcgat
     1981 ccggccctgc aggaaaaggc caggcaggtc gcccgaaagg tgaaaagctt cagcgaattc
     2041 atgcagccat ttattcggga gtccccgcct gccaaattcc tttccggcaa agtgacctat
     2101 cacgacccct gccatctgag ccggtaccag aaggtgaccc aggagccccg cgctcttttg
     2161 aaaaggattc cggggttgac ctatgtggaa ctcccggaag cggatcgatg ctgcggaggc
     2221 gcgggaagtt acaccatttc ccggtatgac caatcgatga aagttctaga ccgcaagatg
     2281 gagctcgtca ggaagaccgg ggccgacatt ctcgcaaccg aatgcccgtc ttgcatcatt
     2341 cagctcagcc acggagcccg ccgtgccggc ctggggactc gagtcgtcca tctcagcgag
     2401 ctattacggg aggtgattta atccttcgtc ccgcacccgg ggtttcgccg agaggctcag
     2461 ccgaaggtcc ggccctctga agcagggctc atgacccgca taggttcaac cttttcccga
     2521 ttgctaaaaa agcccgatgg agaaagccag caggaattgg cccaggtagt agagtggcag
     2581 gccgatcaat cgattgaaaa aatccccttt gataaattgg tttcgggcga cgaataggtc
     2641 agagaggtaa aataggaaag ccccggccag aacgaacaat ctccctccat aggaaagaga
     2701 cgtcccggca aaaactgccc aggccccggt caccatcgag gtgatgacca aaatgtagat
     2761 gcctacggga acgagcatcg ccttcaagtg gggctgcaac catagaaaag cgaaaagact
     2821 gaataaccag aagagaatcg ccgttggcac agcccaggcg gaaagcccta tgagcctgga
     2881 aaaggctatg atgtaaaaaa tgtgcccaag cagaaaagca accagacccg cccgaaaagc
     2941 tttctccccc ggccaaccaa gacagacatc tccgatcaaa caaagaacca ggccaacaat
     3001 gaggcagaag gaataatccg gaagggaaga agattgccga aaagcgaccc agacgaacat
     3061 gcaggaaagg atcgtcttgg tgatgaggcg gagatttttc tcttcccgtt tctcatggat
     3121 tagcaggagc aacaacagaa tcagactaat gacgagaatc accaaggtaa tcatcttgcc
     3181 gaaagaatag aaactcttca gcggcagtca attccatctt caactacagg tttcagggcc
     3241 gaaaagagcc cgcaatactc gggtaatcta gcacaacaga aagagcggtg taaatccccc
     3301 acgggcttgc ctccctttac ctttgggatg cctttttttg tggcacctgg acaagacagt
     3361 ggtttgaagg caggatcagc gggtggcgaa aactcggtga aagactcatc ccttccgtat
     3421 agagaggcgg taaaggcagg accatccgcg caccctatgg ccgtttccgc caggatcgat
     3481 gaaagaatct ttgactgcca acaaccacgg ccttgccatc gggccgggca cggtttccaa
     3541 ttaatggaca ttgatctgat ttctggaaag tgaagggagg catctgacac ggattctcgc
     3601 tggaagaagg ggcttgccac ccaaggtctg aagaatcggc agaaagagtt gcattgcaca
     3661 cctcggttat ccccaagtaa aggatttagg ttccatagaa tgcgggtcga taagaactac
     3721 agccccatat cttaaagtct tattagggat acgaatgcct gggtctcagt tctgggggcc
     3781 ttctttcccg gaggaaaagg aaccccggcg aaatattcct gtccttgaaa agttttgagt
     3841 ttatccatga catagccgat aaaaaggata aacttcgggg agaattgtag atatgatttc
     3901 cctcacccga ttgaacaaca ccggtctggt gataaacccg gatttgatcg ttttcatgga
     3961 ggaaactccg gacacgatca tcaccctttc aaatggagag aagatcgccg tacaggagaa
     4021 aataaaagag gtgatccgcc gggttatcaa tttccgaagg tgtattttca atccctcgat
     4081 tcccatcgaa taaagatatg gataaagcga ctctgatcgg tctatttttg ggttttgcag
     4141 cgatcattgg ggggcaggtt ttagaggggg gaagcctcca ttcgatcatg cagttcaccg
     4201 ccgcagtcat cgtcatgggg gggaccttcg gggctgtttt cgtcagctac cctttccaag
     4261 atgtgatgga ggggtttcgg ggggtccgta aggtgctcaa ggaacctcct caagacccct
     4321 tccagataat tcagcaaatt atcacctatg ccaacaaagc ccggcgggaa gggattctat
     4381 ccttggagaa ggagatcaag aacatccgag accctttcct ggctaaagcc ctgaccatgt
     4441 ccgtggatgg catcgaaccc cacgtcatcc gggaagccat ggagtcggaa ctggaatacc
     4501 tgaacgagta tggaaaggtc agctcaaaag tctggcgcgc tgccggagga tatgccccta
     4561 ccatcggaat cctaggggca gtcctggggc tcattcatgt gatggaaaat ctttccgacc
     4621 ccgggaagct tggggcaggc atcgcggtcg cctttgtagc cactgtctat ggggttggat
     4681 cggccaacct cctgttctta cccatggcca ccaaactgga ggtgcggcac cgccaggtga
     4741 tgattctcaa cgaaatgatc ctggaagggg tggtggcctt gtctactgga gagaatccga
     4801 ggttgatcga agagaagctg acctccttcc tctccgactt gcagaaagaa aaacgctcaa
     4861 cctccgtttc caaggctccg gccggggcgg cggcaggcaa aactctgcag gaggaatcag
     4921 ggtaatcaag agggatcgac agacaagtaa gattgattct cccggagcgc catgaaaaaa
     4981 aggaaaaaac atcaagaaga agagcacgag aacctggaga ggtggttggt ctcttacgcg
     5041 gatttcatca ccctcctctt tgctttcttt gttaccatgt acgccatctc ccgggtggat
     5101 gagaggaaac tcggctccat ggtggaatcc ttgcatcgag ccctgggctc cacgatgctc
     5161 acccaagcga gtcacccgga acccggggcc tttcagagtt ccgcctaccc cgtgaatgta
     5221 gctatcgtcc ccaccccgag agagaaaggg gtcagcagtg aggatttcga aaaactggcc
     5281 caggatatcc ggaagaaaat ggccgaacgg atggagcacg acgggagtta tggagaggct
     5341 agtctgaatg agctcaaatt catcatcgac aagcggggtc tggtccttcg cttctcggaa
     5401 cgtttcttct ttgattcggg cgatgcctcc atccgtcctg aagtggtccc cttgctcacc
     5461 gttctggccc aatctctaga ggcccttccc aatcaggtcc ggatcgaagg ccacacggac
     5521 agtgttccca tccgcaccac cagattcccc tcgaactggg agctttccac cgcccgggcc
     5581 acctcgatcg tgcactactt cctcacccac catcgattcg aacccaaccg gctctctgct
     5641 gcgggatatg gggaattccg acccatcgcg aaaaatcaga ctgccgatgg acggtcccag
     5701 aaccggcggg tggatatcgt catcttgagc gcaaaggagg aggaaaacga accggggatg
     5761 gaaaagaagg tgtatccgac catctaaagg ctagtcaatc cctagaccgg aaagctgacc
     5821 tcaaaaaccg tctctccttc cttggaggaa tagtgaaagg aggccccata cggcgcgaga
     5881 agccgacggg ccacgaataa acccagtccc gcatgtttcc tgcctttcgt ggtaaaggag
     5941 ggttcgaata ggcggggttt ggcttcctca gggatcccgc atcccgtgtc ttgaatggtc
     6001 acctgcacct gccgcccctg gacccgggtc ctcagaatca actccttccg gcgggaattc
     6061 tccatggcct ccagggaatt ctggattaaa ttccacaagg cttgactgaa atccatatag
     6121 cttcccctga gaagaggaag ggaagaggcc aggtctttat ccacctttac ctggtgttta
     6181 aagaacagat cgtgatagag aagggaaagt tcttcttcca gaatatcatt cagctggatg
     6241 gattgaggag actcctgctc gtcgtggatt cccttttgga tcagcagctc cagcatcccc
     6301 ttgaatcgac tgatttgacc taggcatttt tccatctgct cctggacctt atcctccccc
     6361 gggggcaaca tccgctggag gagttccatc tgcatggaca ggatttgaag ggagccgttg
     6421 aggttgtgga tgattccctt caaaagagaa ccaagggtgg cggctcggtg ggaatcgatg
     6481 aaagccgcga ggagtttttg ggcctgcggg tcaagaatgg cttcttcgtg agaattgggg
     6541 gtcgccacag aaataaattc ctttctagga tcgggatccc gattcccttt ctccagcgat
     6601 tattttttct ataccatacg ccacgaaaaa atcaaccgga aatcaatcct tcaaccctct
     6661 cggtagcggg aagccgatac agaatggcca gaaacagcca aggaaattgg atcggcgcaa
     6721 gaagggtttg ggggagagga gcagggcttg ccttcattcc ttgaaggatt ggaagagaat
     6781 tctttttagg gaggtaaact ccgtttcgct caagaggcct tcccgtttgt actcgatcaa
     6841 cttctgcagc tcctggaaag cctttccccg ctcttcccgc tctttaaaat cgtagtccgg
     6901 gggaatcaac tggttgagga ttacacccat ctcggtgagc ttatggtcca aggccagaga
     6961 ggggaccgat tcaaccgggg gatggaattc cggagacggc tttttcaggc ctttcatctc
     7021 ctccatggca tccttcaatt tctggaggag acgccggttt tcccggatta aggcgatttt
     7081 atcgccggcg ttcttgacga tcacctccaa ctcatccagc ttaaagggct tacggatata
     7141 gtcataggcg cccccccgaa tggcctgaat ggccgtatcc agagaggcgt agccggtcat
     7201 gatgacgacc accccgtccg ggttgatttt ttttgatttt tctagaacct ggaggccatc
     7261 cccaccgggc atcagcaagt cagcgatgat aatgtcgaaa gaagggtctc tttttaaagc
     7321 ttcaatcagg tcctcgccat tggcgaagag gcggatggac cttttctcct gggccaagga
     7381 ctttcccaag gtttcccgga aatcgtcgtc gtcatcggca atccagatgc gaaaagggag
     7441 gatttcagaa tccatatcca tgggagaagc gagtcaaggc cggaggacca atcggaagat
     7501 tgccgctcgg cctccttttt atcgttgaat gtccatgaag cggggggcag agccggcctg
     7561 ttggaggaac catcgggcgg ctgcccattc ctcgtgcgtc gttgccaatt cttttttgaa
     7621 ctcttccggc aggagcgccg cctgggcaag gcattgagca ttcaagtcag cggcttcccg
     7681 caggagggcc tttaggttat ggacggccgg atcctcatct gcctcagggg ttccaccggg
     7741 cccagcttgg gaggtcccgg aagacgcctg gagaattttt cgatcgatat catcgatgga
     7801 atggaccagg ttctcgcgct tttcgagttg ccggcctatt tccccgaagt ctcgggtcaa
     7861 aaggcattgg tagagagtct cgctgatggc aacaaactcc cacagggctt cgac
//
LOCUS       DASPRR010000327         4699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_883093, whole genome shotgun sequence.
ACCESSION   DASPRR010000327 DASPRR010000000
VERSION     DASPRR010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883093"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..508
                     /gene="ybeY"
                     /locus_tag="VEL68_12160"
     CDS             53..508
                     /gene="ybeY"
                     /locus_tag="VEL68_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXZ36765.1"
                     /translation="MASKVSVVNRQDRVPVDRRKIGAAARRILKTLGYGGYDLAVVLV
                     DDREITRLNRQYFRRNRPTNVISFPMMDETPAPLHSRILGDVVISADTARRGAEEVGK
                     KAEEEILFLLIHGILHLTGYDHEGSVAERRKMEAKEKEFFKALNRPLRR"
     gene            582..2438
                     /locus_tag="VEL68_12165"
     CDS             582..2438
                     /locus_tag="VEL68_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXZ36766.1"
                     /translation="MNMREVRKLIPETMTAEERSLAAVALEEVDRHPVFPILVTAAPR
                     LYGITQAEAWRKHEVARDAIIRCYKEFGYDFGSKPDYYYPQLPGKFAGAPVRNLIPGK
                     QIAEDDLYQIDERVLFPREDYDQIAALGWNGYWEARYPTISKKTIEQLTFMQKATNQM
                     YYEDLKICESQGVPIFMGVAVDSVMMAFSLCRTLTEFTRDLYEVPGKVEAAMRASCDD
                     LISTAIQVCKSSNKMLAFIVLERGSGFYYRLDVFERFEWPFLRRYVDAFLAEGITPWL
                     HLDTDWGINLPYFTKLPKGKCICDLDGTTDIFRAKEILKGHMCISGDVPAALLSLGKP
                     EDVEAYCKRLIDEVGEGGGFMLTTGCECPINVKRENLRAMVETGKSYVGKKGRARLVQ
                     PVEKRPEAPRVSLPKGKIAEALGRLQYDDVGKWVDRAIQDRRPPLEILEECRAGMEMV
                     GKLYSSGEYFLAELIMSADLFKKVVGKVEPLLSGQKDQDFLGSVVIGTPKGDIHDIGK
                     NIVATLFKASGFQVHDLGVDVAPEDFVRKVADTGAKVVAMSALITPTFESMKKVVDLL
                     KGKKLREDRFVILGGGPTTKTVQDYVGADAWSLDPKAGVDLCKDFVLARGLK"
     gene            2472..3404
                     /locus_tag="VEL68_12170"
     CDS             2472..3404
                     /locus_tag="VEL68_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXZ36767.1"
                     /translation="MEVLLVAGFLGAGKTTLIRHLLTSPPKGLGKLALLVNEVGKIGL
                     DGSLLSGLNVDVVELTSGCICCSIKTDFFKAVEEIYHQFAPSHLIVEPTGVAQPGEIL
                     DVLSQPPLKDFCRVQGIVTIVDAGFFKAREMLGSFYDRQILSADVILLNKIDLVGGAV
                     ALGIEARLEEMNPRAKVFLTRNCSVEPARLFEGLSSAGEKTGKAGFRDENLEHQGFQS
                     FTFEDHGIMDRTKLIRFLESLPASLFRCKGWARFPDSSVIVNYSGGNYRLEPTQDSHP
                     TALVLVGRKCPQDEILEALRKCVIGESILEQIPR"
     gene            complement(3491..4063)
                     /locus_tag="VEL68_12175"
     CDS             complement(3491..4063)
                     /locus_tag="VEL68_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HXZ36768.1"
                     /translation="MEFKGSKTEKNLLAAFAGESQARNRYTFFASEARKAGYEQIAAL
                     FLETAENEKEHAKLFFKLLKGGEVTITAGYPAGVIGDVAANLKASAEGEKFEWSTLYV
                     NFAEVAKQEGFTDVCQTFTQVAKAEKFHEARYRALLKNVQGGKVFKKDSPIKWHCRNC
                     GYEHEGKEAPDKCPVCAHPQAFFEVLAENY"
     gene            4496..>4699
                     /locus_tag="VEL68_12180"
     CDS             4496..>4699
                     /locus_tag="VEL68_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36769.1"
                     /translation="MVLTPRQREILDFIQTFTAKEGYPPTVREIAGHFRIDYRAVIDH
                     LRALERKGSLTRSSKSRGIQVFRP"
BASE COUNT         1042 a         1217 c         1382 g         1058 t
ORIGIN      
        1 cctccccctg tatggaggag agggcaaaga ggaggggggc gattaaccgc cattggcctc
       61 gaaggtttcc gtcgtcaatc gccaagaccg ggtgcccgtc gaccgacgca agatcggggc
      121 ggcggcccgc aggattctga agaccctggg ttacggggga tatgatctcg ccgtagtcct
      181 ggtcgatgat cgggagatca cccgcctgaa ccgccagtac ttccgccgca accgtcccac
      241 caacgttatt tcttttccca tgatggatga aactcctgcc cctctccact ccaggatttt
      301 gggagatgtg gtcatctctg cggatactgc ccgacggggc gcggaagaag tcggcaagaa
      361 agccgaagag gaaatcctct ttcttctgat ccacggcatc ctacatctga ccggctatga
      421 tcatgaggga tcggtggctg aacggagaaa gatggaggcc aaggaaaagg aattttttaa
      481 ggcgttgaat cgccccttaa gaaggtaatc accatcggtc tctcttttcc tgcgccggtg
      541 aaaagtttcc cgggatttga ggggtaggca gggagacaaa aatgaatatg agggaggtca
      601 ggaaattgat tcccgaaacg atgactgccg aagaaagatc cctggcagct gttgccctgg
      661 aggaagtgga ccgtcatccc gtgtttccca ttttggtgac cgccgctccc cgcttgtacg
      721 gaatcaccca ggccgaagcc tggcggaaac acgaggtcgc ccgggacgcc atcatccgct
      781 gctacaagga attcgggtat gatttcggca gcaagcccga ttattattac ccccagctgc
      841 cggggaaatt cgccggggcc ccggtccgca acctgatccc gggaaaacag atcgcggagg
      901 atgacctgta tcaaatcgac gagcgggtcc tgtttccgcg ggaggattat gatcaaatcg
      961 ccgccctcgg atggaacggg tactgggaag cccggtatcc cacaatcagc aagaaaacga
     1021 tcgaacagct gaccttcatg caaaaagcca cgaaccagat gtattacgaa gacctgaaaa
     1081 tctgtgaatc ccagggagtc ccgattttca tgggcgtggc cgtggattcg gtcatgatgg
     1141 ccttctccct gtgccgcacc ctgacggagt tcacccgcga cttgtacgag gtgccgggca
     1201 aagtggaggc ggccatgcgg gcctcctgcg acgatctcat ctccacggcc attcaggtct
     1261 gtaagtccag caacaagatg ctggctttca tcgtcctgga gagggggtcg ggtttttatt
     1321 accggctgga tgtgtttgag cggtttgaat ggccttttct gcggcgttac gtggatgctt
     1381 tcctcgcaga ggggattacc ccttggttgc atctggacac ggactggggg atcaacctcc
     1441 cctacttcac caaactgccg aagggaaaat gcatttgtga cctggacgga acgacggata
     1501 ttttccgggc caaggaaata ttgaagggcc acatgtgcat cagcggggat gttcccgcag
     1561 ccctcttgtc cctgggaaaa ccagaggacg tggaagcgta ttgtaagcgg ctaatcgatg
     1621 aagtgggaga gggtggggga tttatgctca ccaccgggtg cgaatgcccc atcaacgtga
     1681 agagggaaaa tctccgggcc atggtggaga ccggaaagag ctacgtgggg aaaaagggaa
     1741 gggccaggct tgtccagccg gttgaaaaaa ggcccgaggc tccacgggtg agtctcccca
     1801 aggggaaaat tgccgaggcc ctgggccgac tgcaatatga cgacgtgggg aaatgggtgg
     1861 atcgagccat ccaggatcga agacccccct tggagattct ggaagaatgc cgggccggga
     1921 tggaaatggt gggaaagctc tacagttcgg gggaatattt cctggccgag ttgatcatgt
     1981 ccgcggacct cttcaaaaag gtggtaggga aagtggagcc tctgctgtcg ggacagaaag
     2041 atcaggattt cctggggtcg gtggtcatcg ggacgcccaa gggagatatc cacgacatcg
     2101 ggaagaatat cgtagcgact ctgttcaagg ccagcggctt tcaggtgcat gatttggggg
     2161 tggacgtagc tcccgaagac ttcgtccgga aggtcgccga cacgggcgcg aaggtggtgg
     2221 ccatgtcagc cctgatcacg ccgacctttg aatccatgaa aaaagtggtg gatttgctga
     2281 aaggaaaaaa attgcgggaa gacaggtttg tcatcctggg gggagggccc accacgaaga
     2341 cggttcaaga ctatgtcggc gccgacgcct ggtccctgga cccgaaggcc ggcgtggatc
     2401 tgtgcaagga ttttgtgctt gccagaggtt tgaaataaga agctcaggtt ttttggggga
     2461 aatcctgatt catggaagtt ttgctggtgg ccggcttctt gggagccggg aagaccacgc
     2521 tgatcagaca tcttctgact tcaccgccga aggggctcgg gaaattggcc cttctggtca
     2581 acgaggtggg gaagatcggg ctggatggat ccctgctgtc gggcctgaac gtggatgtgg
     2641 tggagttgac cagcggctgc atctgctgca gcataaagac cgatttcttc aaggcagtgg
     2701 aggaaattta tcatcaattt gcaccaagcc acttgatcgt ggagccaacg ggggtggccc
     2761 agccgggaga gatcctcgat gtcctcagcc agccgccgct gaaggatttc tgccgggttc
     2821 agggcattgt caccatcgtg gatgccggct tcttcaaggc cagggagatg ctgggctcct
     2881 tctacgatcg gcagatcctg agcgcggatg tgattctttt gaataagatt gatctggtgg
     2941 gtggggcggt ggcgctgggg atcgaggccc gattggagga aatgaatccg cgggccaagg
     3001 ttttcctcac ccggaattgc tcggttgagc ctgcgcggtt atttgaagga ttgtcttctg
     3061 ccggggagaa aactggaaag gctggtttca gggacgaaaa tcttgaacat caggggtttc
     3121 aatcatttac ctttgaggat catgggatca tggatcggac gaagctcatc cgattccttg
     3181 aatccctgcc cgctagcctc ttccgctgca aaggatgggc ccgattcccc gactcctcgg
     3241 tgatcgtgaa ttattcaggg gggaactaca ggctcgagcc cacgcaggat tcccacccga
     3301 cggccctcgt cctggtggga agaaaatgcc cccaggacga gatcctggag gcattgagga
     3361 aatgtgtcat tggagaatcc atcctcgaac aaattccgag ataggtcgta ggggcgcccg
     3421 gcggctcggc tgagctcgcc gaagtccgag cgcccttcaa tccgaaatcc gcattccgca
     3481 atccgcaatt ttagtagttt tccgccagca cttcaaagaa ggcctgggga tgggcgcaga
     3541 ccgggcattt gtccggggct tctttccctt cgtgctcgta gccgcagttc cggcagtgcc
     3601 actttatcgg tgaatccttc ttgaagactt ttcctccctg gacatttttc aaaagcgccc
     3661 ggtaccgggc ctcatggaat ttttccgcct tggcgacctg ggtgaaggtc tggcaaacat
     3721 ccgtgaaccc ttcctgcttg gccacttcgg caaaattcac gtagagggta ctccactcaa
     3781 atttttcccc ttcggcggag gctttcaggt tcgcggcaac atctccgatc acgcccgcag
     3841 ggtacccggc ggtgatggtt acctcccccc ccttgagcag cttgaagaac aacttggcgt
     3901 gttccttttc attttccgcc gtctccagaa agagagcggc gatttgctcg tatccggcct
     3961 tcctggcctc gctggcaaag aaggtgtaac ggtttcgggc ctgcgactcg ccggcaaaag
     4021 cagccagcaa attcttctcc gtcttgctgc ccttgaattc catgattcct cctccttatt
     4081 ttggattacc gttaaaattc tgatgattcc ataaaaactc gtcactccgg tggaaaccgg
     4141 agtccaggga acttataacg agtaaaaaaa ctggatcccg gctttcgccg gaatgccggg
     4201 aaagcctgct ttttgacttt atacaggatt gttcattcta atcattttat tttaacccgc
     4261 cacctgattt tccgcatgtc ccttttccga ttccgtaggg gcggccccct gtggccgccc
     4321 tatgattttg gtaacctgca ggggcgccct aagatttttc ttgacaactc atcaaatgat
     4381 gagtatatta agacaaagtc atgagtttcg atcctcaaaa cccaaatcgc caaatttttc
     4441 cgctagttcc gaacatttgg gttttgaatc tgtttcggat ttaaggaggt catagatggt
     4501 tctcaccccg aggcaaagag aaattctgga cttcatccag acctttacgg caaaagaagg
     4561 gtatcctccc acggtgcggg agatcgccgg gcatttccgg attgattacc gggcggtcat
     4621 cgaccacctg cgggccctgg aaaggaaggg aagcttgacc cgttcctcca agtcccgggg
     4681 gatccaggtc ttccggccc
//
LOCUS       DASPRR010000328        19291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_393795, whole genome shotgun sequence.
ACCESSION   DASPRR010000328 DASPRR010000000
VERSION     DASPRR010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19291)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19291
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393795"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1001
                     /locus_tag="VEL68_12185"
     CDS             165..1001
                     /locus_tag="VEL68_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXZ36770.1"
                     /translation="MAQIRVDSEKCTACGTCSTACPAIFILEGEKVRTFDEEHCTLCG
                     HCLALCPVDAINIRELNPEEFSDLPQDQKISPETLAVFLRSRRSCRVFADREIPRQAL
                     EKLIDMARYAPSGHNSQNFEFIVINNRQLVRTLAQRTAVFYGNLYKMLSAPGVSIPPW
                     LQTHMRGFRLNWEYYQAGKDRIFRNAPALIVIHALAENNSSAQNCHLAMAHIMLQAHA
                     MGLGTCINGYFTTAAERDPSIVKELEVPKENKIFTCCTVGYPALKFRKLVQRRPPSVR
                     WL"
     gene            1213..1755
                     /locus_tag="VEL68_12190"
     CDS             1213..1755
                     /locus_tag="VEL68_12190"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HXZ36771.1"
                     /translation="MEIKIGNATIDLLEGDITSQGTDAIANAANRSLLGGGGVDGAIH
                     RAAGPQLLAECRTLGGCETGDAKITKGYNLKARHVIHTVGPIYHSSAKKAPELLASCY
                     RRSLEVASQNKLKSIAFPSISTGAYGYPLEEAAPIAIKTVMDYLKAHPDIQLVRFVLF
                     GRDAYQAYEKALKEQAKKSA"
     gene            complement(1860..3218)
                     /gene="phrB"
                     /locus_tag="VEL68_12195"
     CDS             complement(1860..3218)
                     /gene="phrB"
                     /locus_tag="VEL68_12195"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HXZ36772.1"
                     /translation="MIHPERVRKLNDRSIQKGRFILYWMQASQRAECNHALEYSIRQS
                     NQQKLPVATLFVLTDGFPEANLRHYTFMLEGLKELKGRLEERGVSFLVLRGSPGKEVV
                     KMSRQASMVITDCGYLRIQREWRKEVAEKSPCPVVQVETDVVVPVEEACPKEAYSAAV
                     LRPKINSQLRKYLVPLSETSANHSLRNQDFSGVHLRKTIPSILSPLSIDRTVKPVSHF
                     RGGTSEAKSLLEDFIAHKLRDYGELRSDPSLDCVSHMSPFLHFGQISPLYIALRVQEA
                     GRIPAKTRDAYLEELIVRRELAMNFAHYNPAYDSFQAISDWAKRTLKAHQRDKREYIY
                     SQNELEEAKTHDPYWNAAQREMVLTGKMHNYMRMYWGKKILEWCAEPEEAFRTAVYLN
                     NKYELDGRDPNSFAGLAWCFGKHDRPWAERKIFGQVRYMNDAGLRRKFKIDDYVKKAE
                     SL"
     gene            3458..4696
                     /locus_tag="VEL68_12200"
     CDS             3458..4696
                     /locus_tag="VEL68_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36773.1"
                     /translation="MKKLGYFSVFALVMVLLLGTVLAEAQDVIRIGSIYPLTGAIASS
                     GLRSKHAIETAIDVINNKYDFDIPFAQTQGIPNLKGAKLQVIWGDSQASPEVGKTEAE
                     RLITQEKVPLLLGAYQSGVSKSASFAAERFKIPFVCAESSSAALTERGLKYFFRIAPT
                     DAEDSIAFFEFLEQMKAKGKKVERVAIVYENTEFGVHAAGEAKKQAEKRGYKIVVDVP
                     YPFKSTDVKSEVLKIKAAKPDVILQASLLGDMMLFAKTFKEQKVAFNANLSYCGGYHD
                     PSFVQLMKKDAEFYMGQCTFSPDFITKRPVLAKINEMYKKRSGVDFDGVTIEGFTSVL
                     VIADAFNRAQSTDPEKVVQALRTTDLKTPTLPGDAVKFNEQGQNIKIISNISQVQNGE
                     YRVIWPASMAAVDLVWPFKY"
     gene            4813..5679
                     /locus_tag="VEL68_12205"
     CDS             4813..5679
                     /locus_tag="VEL68_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36774.1"
                     /translation="MDIEILFQILISGLLMGLIFALIAVGLTLIWGVMDILNFAHGEF
                     LMLAMFFSFWMFSLWAIDPLWSLPVTVALLFAIGVGTYYFIIKKVLNAPGLTALLATF
                     GLSILIKNLAQFFWTPDFRQVQGAILGEKTLSILNLNVSLPELIAACGSGLITYLVYY
                     LIFRTKTGRAIRAAALDRDTALLMGINIDRVYALTFGLGGACVGVAGALLMNFYYVFP
                     SVGSLFGILAFVTVALGGFGNITGAFYAGILIGVVETAGGVFVSPVFKYTIVFMMYLV
                     IISIRPKGLFGW"
     gene            5683..6648
                     /locus_tag="VEL68_12210"
     CDS             5683..6648
                     /locus_tag="VEL68_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36775.1"
                     /translation="MDTSALALLKKPWVLIALLVLLFLPFTTSNIHFHHVLIMFMIFA
                     TLSQAWNVIGGFAGQVSFGHATFFGIGAYAALVLLKRFDLTPWVGMLIGGGVAVIIAI
                     LIGYPVFRLKGHYFAISTFAVAEITERLFANWDFVEGAIGLPAPVLPEGLVNFMWYKT
                     KMPYFYIAFVLFVAVLLLVYRMTRSRLGFYLRAIKQSHEAAEGLGIDTTKYKMVAMAM
                     SAFLTAICGSFYAQYILFIDPPSVLSLDISIKIVLITVLGGAGTVIGPILGAAILIPL
                     SEYSRILLGGTGKGLDLIVFGALILFISVYQPQGLVGFLRKTGKS"
     gene            6650..7372
                     /locus_tag="VEL68_12215"
     CDS             6650..7372
                     /locus_tag="VEL68_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36776.1"
                     /translation="MEILNVKNLTMKFGSLAAVDDVSLSVGKGEIIGLIGPNGAGKTT
                     FFNCLTGYLTPQNGAVQFDGHPITGMAPNRICRLGLARTFQIVQVFREMATWENVIVG
                     AFCRTANSDEAFQETMEILKFTGLYEKKDSLVGNLTIADHKRLEISKALATKPHLLML
                     DEAMAGLNATETIEAIELIKKIRGRGITLIVVEHVMEVIMSISERVVVFDSGKKIAED
                     TPEKIVKNPRVIEAYLGEAYHA"
     gene            7365..8069
                     /locus_tag="VEL68_12220"
     CDS             7365..8069
                     /locus_tag="VEL68_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36777.1"
                     /translation="MLEVKKICVSYLGVPVIREVSFKVEEGQMVSIVGSNGAGKSTIL
                     KTISGLLRPLSGEITFLGKRIDTLPPHAITSAGISHIPEGRKIFSKLSVLDNLLVGAH
                     TRDSNAEVQQSLEEIYTLFSILKERKDQRGETLSGGEQQMLAIARGLMSKPKLMMLDE
                     PSLGLMPSLSVKVIEILKRISSNGTTILLVEQKVRDALELADRAYVLQTGAIVMEGSG
                     AELLKSDMIRKSYLGM"
     gene            8106..8582
                     /locus_tag="VEL68_12225"
     CDS             8106..8582
                     /locus_tag="VEL68_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ36778.1"
                     /translation="MRGSNKNNKSLFSKLHHLSVVVRNMDEAIKFYESIGIGPFEDYP
                     PLTEYVKLNVQDETGFLNLKFKIARVGDIQIQLCQPGEGKSLYKDFLEKKGEGVYHLG
                     FVVDDIDDSEASLKKLGLRVLSSGRRVDGSGFSYFDTAGKAGVVLLVRQSPGGKQK"
     gene            8849..10642
                     /locus_tag="VEL68_12230"
     CDS             8849..10642
                     /locus_tag="VEL68_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ36779.1"
                     /translation="MNGYAGKLLRVNLSNGKVSKEEISDSMKRDFLGGRGFAVKLLWD
                     EVRNADPLSEKNKIVFATGPLTGQSIPSSGKMIVASKSPLTGGYGDGNIGTMASVYLK
                     KAGYDVVVVEGKSEKPCYLLIEDEKVKIVEAPELWGKNTFEAQDLLEKKHGKNSGILL
                     TGPAGENLVRISTVMSQKGRAGGRPGMGAVMGSKKLKAVVIKGTGSVGNFDDNNLQEM
                     GKKGYAEIKAKESYDFWMKQGTMQAFDWANENACLPTYNYREGVFEFSKEMDGYVVEK
                     LHAGRKGCPLCNMQCGHIIKDSSGQGAELDYENVAMLGPNIGLGSLPKVALLNRMADE
                     WGLDTISLGSCIGFLMEASEKGIITEKIKWGDFEAAQKLTMEIGKGAGLGKLFSYGVK
                     GASETLGKGSQKWAMQVKGLEVSAYNCQACPGMALAFGTSPIGAHHKDAWIISWEIAN
                     DRFSYNKPKVEKLIEFQRIRGGFFESATVCRLPWVEVSFGLDWYPKYFESITGEKKSW
                     DDIYRLGDRIYNLIRAFWIREVPSFDRKWDYPPERWFEEPHSGGKMKGIKVEREKYEQ
                     MLSWYYELRGWDQNGIPLKRTLDSLGLKGVS"
     gene            10659..10877
                     /locus_tag="VEL68_12235"
     CDS             10659..10877
                     /locus_tag="VEL68_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ36780.1"
                     /translation="MAELKFFGYLADIAGARAKEMCLEKPVALREILPISFPETNIII
                     LINQEVGHLDSLIRNEDRVALMPVLSGG"
     gene            10943..12790
                     /locus_tag="VEL68_12240"
     CDS             10943..12790
                     /locus_tag="VEL68_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ36781.1"
                     /translation="MAGISYRILEVDLSSGQSKVIEYGEEALRKYFGGSGLAASILLD
                     RFDSSLVPFHPQSPLIFMTGLFTGIPVPCGCRVSICAKSPLRIWGEANAGGYWGPELK
                     FTGFDGLIITGKAKEPSYLWVTPKEAEIRSAKALWGKGTFETTEELAKQTDPKARVAA
                     IGPAGESLSYMAGIITGGLEARVMARSGPGAIMGSKNLKAVVVRGDQKPFIINQKILQ
                     EVTKEFLLNLRFVEGLSKFGTASVIESKEAAGVLPIKNFTQAHWDKAKKIAGPALVAK
                     YLERHYGCYSCPIRCGKDVKGPEGPYAGVVGHGPEYETLSAFGSLILNDDLKSLIHLN
                     FLCNDLGLDSMSAGITAAFAIESNLRGYLKENDGLDLQWGNAQSVGVLLEKIARRQGI
                     GAIFADGVKKASRALGKGTEYFALHSKGVEVSLSNPTPTVSLALSWATSNRGACHLEG
                     FSHQVEGGVPFPEMGYTQGMDGISGEGKGRMTALMQDYMAVFNALGLCKFLFFARVSH
                     ETLCKWLKGLAGWDLTSKDLMEVGERLYNLKRAYNVLMGVNAEKDVVAPRILENLRPE
                     KPISEGRKLFFDMRKEYYEARGWDEKGIPTRERLLSLGLEGAAEKIKRK"
     gene            12953..13237
                     /locus_tag="VEL68_12245"
     CDS             12953..13237
                     /locus_tag="VEL68_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ36782.1"
                     /translation="MISFGCGTAALFSSVSNNMLIHVKTLGTLKSLEEGPKVISLEVP
                     EGAPVSDAIEKLGMKDWEVGLVLINGTHATKESILKDQDQLTLIAPIVGG"
     gene            13386..14084
                     /locus_tag="VEL68_12250"
     CDS             13386..14084
                     /locus_tag="VEL68_12250"
                     /inference="COORDINATES: protein motif:HMM:NF017459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNA1162 family protein"
                     /protein_id="HXZ36783.1"
                     /translation="MTLAGFLLSCTPVRTKNIVDRVYELDPQGKIFVSKTLLENPPKT
                     VAVLPFRSQIGEGRVEGSEALFNSLSRKDPSQPESVGERMRRAFFGQFAQLEFDHVKI
                     SRVDRILRGEGLDTWEKMKSLSPQRLGELLGADAVIIGEVTHFDYYYAFLYSQLAVGL
                     SLEMVDTRTTEILWRVHDARRDHTVRVVYDPIALVVGLFQVGFSMRPINMMRAMDEIC
                     RELVGTIPPPQENQ"
     gene            14150..14734
                     /locus_tag="VEL68_12255"
     CDS             14150..14734
                     /locus_tag="VEL68_12255"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXZ36784.1"
                     /translation="MKIAFVVFNGLTTLDFIGIYDPLTRLKTMGFMPDLGWDICAYTD
                     IVKDDRGLVMVPTAIQEPLQSYDVLVVPGGYGTRTLFQDKNFIEWIKTAEPCKLKVSV
                     CTGALLLGAAGFLRDKRATTHRSAFQELEPFCQKVLDHRIVDEGNIITARGVSSGIDE
                     GLYVVERLAGAEARSKIARQMDYPYTLQAGDVAK"
     gene            14863..15210
                     /locus_tag="VEL68_12260"
     CDS             14863..15210
                     /locus_tag="VEL68_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HXZ36785.1"
                     /translation="MAGIEWLLEIYGCPEETLRSRESLTGLFQTIISAMGLKPLGDSI
                     WHRFPETGGLTGFWLLQESHLAIHTFPEFQSACLNVFCCFERPAIEWESILSEKLDAR
                     EVRVREYLRVYKK"
     gene            15264..15896
                     /locus_tag="VEL68_12265"
     CDS             15264..15896
                     /locus_tag="VEL68_12265"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HXZ36786.1"
                     /translation="MKKEVNCPSCGAKVVFQSVASVMAVCQYCSSTLVRHDLDLENIG
                     KMAQLQADGSPLQLRAEGKYQGDSFTVVGRIQLRFEKGLWNEWHLLFDDQRSGWLGEA
                     AGIYAVSFLTEVGEKIPQFEELRPGESVTLKKASYEVTNMEKAFCIGGEGELPFQIGP
                     GYEAPVADLAGQGKQFATLDYSEEPPLVYMGEYVEFEELHFSRLREIDGW"
     gene            15907..17268
                     /locus_tag="VEL68_12270"
     CDS             15907..17268
                     /locus_tag="VEL68_12270"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HXZ36787.1"
                     /translation="MADPSLTATAPAPATPQVKTLRCSGCGNPLTVRGMERTESIACE
                     ACGSVIDLTDENLRIISTFQSKIKHKPLIPLGSRGKVRGDLFEVIGYLRRAVTVEGVD
                     YEWSEYLLFNPYKGFRWLSEYNGHWNFLKTTTNIPKKKGDGVRYLDKTFLHFQTAESR
                     VTYVLGEFYWKVQAGETCKYTDYVAPPLILSKEQTAQETDWSIGEYIEPETLWSAFKI
                     ASPMPARIGVAPNQPSPYSGKVARLWKLIGYLFLAAVFIHLALFFFSQNKLVFENRFT
                     FQQKDKGKAIVTEFFDISGRMSNVVIKTAASPLDNSWLFLNMALISEDGRAYDFGREI
                     SYYHGTEDGSAWSEGSFSDEATLPSIPSGRYYLRIEPESPAPLVNYHIRVYRDVPRWS
                     FFFLALGALCILPILLIWRLARFEGRRWSESDHPGTLWMRIPDSTPEEETGKEKPKGK
                     VNR"
     gene            17265..17435
                     /locus_tag="VEL68_12275"
     CDS             17265..17435
                     /locus_tag="VEL68_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36788.1"
                     /translation="MKKFIIVYGAAVLLFMGIAEYRGWSLMSYQEQKGIPKSLRENPG
                     TYRSSYGGVLHK"
     gene            17501..17719
                     /locus_tag="VEL68_12280"
     CDS             17501..17719
                     /locus_tag="VEL68_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HXZ36789.1"
                     /translation="MLDQVKIGAFISSLVYSFVGVLVFWVSFMIIDRLTPYHLWKEII
                     EEKNTALAIVVGGIAIGICIIVAAAIHG"
     gene            17750..19246
                     /locus_tag="VEL68_12285"
     CDS             17750..19246
                     /locus_tag="VEL68_12285"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HXZ36790.1"
                     /translation="MTYALLFSVFIIASCGLIYELIAGTLASYLLGDSVTQFSTVIGT
                     YLFAMGIGSYLSRFIGKGLVAKFIAIEIMVGIIGGFSATALFLSFSYAKSLKPLLYFL
                     VTVIGILVGLEIPLLLRILKDRLQFKDLVSQVLTFDYLGALAASLLFPILLAPRLGVV
                     RTSLLFGLMNALVAAGTAWLFRYQLSQLRYIFVQCFLAIALLGGGLIYAERLTALAEG
                     ELYADEIIYARSSPYQRIVITRWKDDVRLFLNNHLQFSSRDEYRYHEALVHPGLQSLN
                     GPRHVLILGGGDGLAVREVLKYDSVQTITLVDLDPEMTRLFSRHEVLTRLNLDSLNSP
                     KVKIINADAFLWLEENQEFFDFVVVDFPDPSSYSLGKLYTTAFYRQLYRCLSERGLAV
                     VQATSPLFARRSFWCVVKTLESVGFRTTPYHVYVPSFGEWGFVIASKQPFRKPDQYLP
                     SLKFLTPELAPSLFHFPKDMAPVEAEVNRLNNQVLVQYYEAEWHKVTD"
BASE COUNT         4448 a         5005 c         5379 g         4459 t
ORIGIN      
        1 aaagggagga ccgaggacgg aaaggatttc cagatcgccc tggagaatct gggaaaggat
       61 gtgaccatgg tcaaggtgcg tgtcggcgtc ttcggagatg aaaagagctc caggaccatt
      121 catgaaacga tcgccaagag gctgagtaaa tagaagggat gtctatggcc cagattcggg
      181 ttgattcgga aaagtgcacc gcctgcggaa cctgctccac cgcctgcccc gccatattca
      241 ttcttgaggg cgaaaaggtc cggacctttg acgaagagca ttgcaccctc tgcggccatt
      301 gcctggccct ctgcccggtg gatgccataa acatccggga actgaaccct gaggagttct
      361 ccgatcttcc gcaagaccag aaaatctctc ctgaaaccct agcggtcttt ctccgttccc
      421 ggcgcagctg tagggtcttt gccgacaggg aaattccccg gcaggcactg gaaaaactga
      481 tcgatatggc ccggtacgcg ccttcgggcc ataattctca aaacttcgaa ttcatagtga
      541 tcaacaaccg ccagttggtc cgaaccctgg ctcagcgaac ggccgttttt tacggaaacc
      601 tttataaaat gctgagtgcc cccggggtca gcatcccacc ctggctccag acccacatgc
      661 gcgggttccg tttgaactgg gagtactacc aggcgggaaa agaccgaatt ttccgaaatg
      721 ccccagccct aattgttatc cacgccctgg cggaaaacaa ctcatcggcc cagaattgcc
      781 acctcgccat ggcccacatc atgctccagg cccatgccat gggactgggg acctgcatca
      841 acggatattt caccaccgct gccgagcgag acccgtccat cgtaaaggaa cttgaagtcc
      901 caaaagagaa caagatcttc acctgctgca ccgttggata ccccgctttg aaattccgaa
      961 aactggtgca acgaagacca ccgtccgttc gctggcttta gaggctgcgg cgcagggaat
     1021 tcatagagcg ctcattccag cgaaaggggt aatccaggcc taacggtgcg atcggggatc
     1081 ctaccctaca tgtttctcgt cacccgatag gtaaggaacc ctgtagggcc tgatccctga
     1141 tcaggccgta acctggatgc tcgcgtccgc gggcatgacg aaatctaaca cgatcttttg
     1201 aaggaggtag ccatggaaat caaaatcggg aatgccacga tagatctctt ggaaggagat
     1261 atcaccagcc agggcacgga cgcaatcgcc aacgcggcca accgaagtct tttgggtggg
     1321 ggcggcgtgg atggagcgat ccatagggct gccgggccgc aactcctggc ggaatgccgc
     1381 acgttgggag ggtgtgaaac cggcgacgca aaaatcacca aagggtataa cctgaaggct
     1441 aggcatgtga ttcataccgt cggcccgatc tatcactcct ccgccaagaa agctcccgaa
     1501 ctcctggcca gctgctaccg acggagcctg gaggtggcca gccagaataa gctgaagagc
     1561 attgcttttc cctccatcag caccggggct tacggctatc ctctggaaga agcagcccca
     1621 atcgccatca aaacggtgat ggattatctg aaagcccatc cggatattca gctggtccgc
     1681 ttcgttctct tcgggcgaga tgcttaccag gcctatgaaa aggcattaaa agagcaggca
     1741 aagaagagcg cttagaaaca agcctttaaa accctaacct taccaagtcg gaactaaaca
     1801 ggatatccag ggggcagcgc cctgtggccg cccgcaaaat gggcaacgag ggggtacccc
     1861 tacagagact ctgctttttt cacatagtcg tcaatcttga acttcctccg caagccggca
     1921 tcgttcatgt agcgcacctg gccaaagatc ttccgctcgg cccagggccg gtcgtgtttg
     1981 ccgaagcacc aggccaagcc tgcgaagctg ttggggtccc ggccgtccag ttcatacttg
     2041 ttgttcagat acaccgcggt gcggaaagcc tcctcgggtt cggcacacca ctccaggatc
     2101 ttcttccccc agtacatgcg catatagttg tgcatcttcc cggttaagac catttccctc
     2161 tgggcggcgt tccagtaggg gtcgtgggtc ttggcttcct ccagttcatt ctgagaataa
     2221 atgtactccc gcttatcccg ctggtgagct ttcagtgttc ttttggccca gtcggagatt
     2281 gcctggaagg aatcataagc tggattgtag tgggcgaagt tcatggccaa ttcccgccgc
     2341 acgatcagct cttccaggta ggcgtccctg gtttttgcgg gaatccttcc cgcttcctgg
     2401 acccgcaaag cgatataaag aggagagatt tgcccaaaat ggaggaaggg gctcatgtgg
     2461 gagacgcaat ccagggaggg atcgctcctg agttcaccgt agtccctcaa tttatgggcg
     2521 atgaaatcct ccagcaggct tttggcctcc gaggtcccac cccggaaatg gctcacaggc
     2581 ttgacagtcc ggtcgatgga aagaggagaa aggatggagg gaatggtttt tctaaggtgc
     2641 acgcctgaga aatcctgatt ccgcagcgaa tggttcgccg aggtttcgga cagcggaacc
     2701 agatattttc tcagctggct attaatttta ggccgaagaa cagccgccga ataagcctct
     2761 ttcggacagg cctcctccac cggaacaacc acatccgttt ccacctggac gactggacac
     2821 ggggattttt cggcgacctc cttgcgccac tccctctgga tccggagata tccgcaatcg
     2881 gttatcacca tggaagcctg gcgggacatt ttcaccacct ctttacccgg cgatcctcga
     2941 agaaccaaga aggaaacgcc ccgttcttcc agccgcccct tcaactcttt cagcccttcc
     3001 agcataaagg tatagtggcg cagattggct tccgggaacc catccgttaa gacaaagagc
     3061 gttgccacgg ggagcttttg ttggttggac tgccggatgg agtattccag tgcatggttg
     3121 cactccgccc gctgggaggc ctgcatccag tagaggatga accggccttt ctgaatcgac
     3181 cggtcattca gcttacggac cctttccgga tggatcatac ccagctcctc aagtcggtga
     3241 gttcattccc ccgagggaga gagaataagg aattcagtat attcggaggg acaggaaatg
     3301 gtcaatgaaa atgcccccaa accagccatt aaaagcctct tgggggccct ttcttttttc
     3361 ttgacaattt tgaaaaaagt gaaggataat accgtcctac gctaagaagt gaggtcaaat
     3421 ccgcttaaaa gaaattccaa agaaggaggg aaggaagatg aaaaagctgg ggtatttttc
     3481 cgtatttgca ttggtgatgg ttctgctttt gggaaccgtc ctggccgagg cgcaggatgt
     3541 aatccgcatc gggtcgatct atcccctgac gggggccatt gcttcttccg ggcttaggtc
     3601 taagcatgcg atcgaaaccg caatcgatgt gatcaacaac aagtatgact ttgacattcc
     3661 ttttgcccag acccagggga tcccaaattt gaaaggagcc aagctccagg tcatctgggg
     3721 ggacagccag gcttccccgg aagtcggcaa gaccgaggcc gagcgcctga tcactcagga
     3781 aaaggttccc ttgctcctcg gcgcctatca gtccggtgtg tccaaatcgg cgagtttcgc
     3841 ggccgagcgg ttcaaaatcc cattcgtctg cgccgagtcc tcctccgcag cactgaccga
     3901 gcgtggcttg aagtatttct tccggatcgc tcccacggac gcagaggatt cgatcgcctt
     3961 ctttgagttc cttgaacaaa tgaaggcgaa ggggaagaag gtggagcggg tggccatcgt
     4021 ctatgaaaac accgaatttg gggtgcatgc cgccggagaa gcaaaaaagc aggcggaaaa
     4081 gcggggctac aagatcgtgg tcgatgttcc ctaccctttc aagtctacgg acgttaagag
     4141 cgaagttttg aagataaagg cagccaagcc ggatgtcatc cttcaggctt ctctgctggg
     4201 tgatatgatg ttatttgcca agaccttcaa ggaacagaaa gtagccttca atgccaatct
     4261 ttcttattgc ggtgggtatc acgaccccag ctttgttcag ctcatgaaga aggacgctga
     4321 attctacatg ggccagtgca ccttcagccc agacttcatc accaagaggc ccgttttggc
     4381 aaagatcaac gaaatgtaca agaagcgttc gggagtggat tttgacgggg taaccattga
     4441 agggttcacg tcggtgctgg tcattgccga tgccttcaac cgggcccagt ccacggatcc
     4501 cgaaaaagtc gtccaggccc tgcggaccac cgatctcaag actccaaccc tcccgggcga
     4561 tgccgtaaag ttcaacgagc aggggcagaa catcaagatt atatccaaca tctcccaggt
     4621 tcagaatgga gagtatcgcg ttatctggcc tgcctccatg gcggctgtcg atttggtgtg
     4681 gcccttcaag tattgagaca agagataaaa tagcgaaaag gggtcccttc ggcccttttt
     4741 cggagtgatc gctgcgagga cttaggcttg aaaatgggct taagtcctcg taatttgtgg
     4801 agatacggaa cgatggatat tgaaattctc ttccagatcc tcatcagcgg cttgcttatg
     4861 ggcctgatct tcgccctgat tgccgtcggg ttgaccctga tttggggcgt aatggacatc
     4921 cttaatttcg cccatggcga gttcctcatg ctggcaatgt ttttttcctt ctggatgttc
     4981 tctctatggg ccattgatcc cctctggtcc ctgccggtta cggttgctct cctctttgcc
     5041 atcggggtag gcacgtacta cttcatcatt aagaaggttc tgaatgctcc cggactcaca
     5101 gccctcctgg ctaccttcgg cctctcgatc ctgattaaaa acctggccca attcttctgg
     5161 actccggatt tccggcaggt ccagggggcc attttgggcg aaaagacctt gagcatcctc
     5221 aacctcaatg taagtctccc ggaattgatt gcggcctgcg gaagcggcct catcacttat
     5281 ctcgtttatt atttgatctt ccggacgaaa accggccgag ccattcgagc cgcagcattg
     5341 gaccgggaca cagccctact gatgggcatc aatatcgacc gggtctacgc cttgaccttc
     5401 ggcctggggg gtgcgtgtgt gggagtcgcc ggggccctgc tgatgaactt ttattatgtc
     5461 tttccgagcg tggggagcct ttttggaatt ctggccttcg tcaccgtggc cctggggggg
     5521 tttggaaata ttaccggagc cttctatgcc ggaatcctca tcggggtggt ggagaccgcc
     5581 ggcggggttt tcgtctcccc ggttttcaaa tacaccatcg tctttatgat gtatttagtg
     5641 atcatatcta ttcggcccaa aggccttttc gggtggtagg aaatggacac aagcgctctt
     5701 gcccttttaa aaaaaccctg ggttctcatc gctctgctcg tgctgctttt ccttcctttt
     5761 acgacgagca acatccactt tcatcatgtt ttgatcatgt tcatgatctt tgccaccctg
     5821 agccaggcgt ggaatgtgat tgggggattc gccggccagg tctcctttgg gcatgccact
     5881 ttcttcggca tcggggcgta tgccgccctg gttttattga agagatttga tttaaccccc
     5941 tgggttggaa tgctgatcgg cggaggagtc gcggtcatta tcgccatact cattggctat
     6001 cctgtgtttc gtctcaaagg acattatttc gccatctcca cttttgccgt ggccgagatt
     6061 accgagcggc tcttcgccaa ctgggatttc gtagaagggg ccatcggcct tcccgccccc
     6121 gttctgccgg agggtttagt caacttcatg tggtacaaaa ccaaaatgcc ctatttctac
     6181 atcgccttcg tcctctttgt ggccgttctc ctcctggttt acaggatgac ccgttcgcgt
     6241 ctagggtttt acctgcgcgc catcaagcaa agccacgaag ccgccgaagg cctggggatc
     6301 gacaccacga aatataaaat ggtcgccatg gcgatgagcg ctttcctgac ggccatctgc
     6361 ggctcttttt acgcccagta tattctcttc atcgatccgc cctccgttct ctccttggac
     6421 atctccatca agattgtctt gattaccgtc ttgggcgggg ccgggaccgt gattggcccg
     6481 atcctggggg cggccatttt gatccccctc tcggaataca gccgcatcct gctgggcgga
     6541 actgggaagg ggttggacct gattgttttc ggagccttga tcctattcat ctccgtttat
     6601 cagccccagg ggcttgtagg attccttcgc aaaacaggaa aatcatagta tggaaattct
     6661 caatgtgaaa aacctgacca tgaagtttgg cagcctggcc gcggttgatg acgtcagtct
     6721 ttccgtaggg aaaggagaga tcatcggcct catcgggccc aatggcgcgg gcaagaccac
     6781 cttttttaac tgcctgacag gttatctcac tccgcaaaat ggggcggttc aattcgacgg
     6841 ccatcccata acgggaatgg ctccgaaccg gatctgccgg ctgggtttag cccggacctt
     6901 tcagatcgtc caggtattcc gggaaatggc aacctgggag aacgtcatcg tcggagcctt
     6961 ctgccgcacg gcgaattcgg acgaggcctt tcaggagacg atggagatcc tgaaattcac
     7021 gggactttac gagaagaagg actctttggt gggcaacctg accattgccg accacaagcg
     7081 tctggagatt tccaaggctt tggccactaa accgcatctc ctcatgctgg atgaagccat
     7141 ggcaggtctc aatgccacgg agaccatcga ggccatcgag ttgatcaaga aaatccgcgg
     7201 ccgggggatc accctgatcg tggtcgagca cgtcatggaa gtcatcatgt ccatctccga
     7261 acgagtggtg gttttcgact ccggcaagaa aatcgcggaa gacactccgg aaaagatcgt
     7321 caaaaatcct cgggtcatcg aggcctatct gggggaggct taccatgctt gaggtgaaga
     7381 agatctgcgt aagctacctg ggggttccgg ttatccgcga ggtctctttc aaggtggaag
     7441 aagggcagat ggtttccatc gtcggctcca atggcgccgg gaaatcgacc attttgaaaa
     7501 ccatttccgg actcctccgc cccctgagcg gggaaatcac tttcctcggg aaaaggatcg
     7561 ataccttgcc accgcacgcg atcacctctg cggggatttc ccatatcccc gaaggcagga
     7621 aaatattctc caagctgtcg gttttggaca atctgctggt gggagcgcac acccgggatt
     7681 ccaatgcaga ggtccaacag agtctggagg agatctatac cctcttttcc attctcaagg
     7741 aaagaaagga ccagcgggga gaaaccttgt cgggtggaga acagcagatg ctggccattg
     7801 cccgggggct catgtccaaa cccaagctca tgatgctcga tgaaccctct ttgggcctga
     7861 tgccttccct gtccgtaaag gtgatcgaaa tcctcaagcg gatcagctca aatggaacca
     7921 ccattcttct cgtggagcaa aaggtaagag acgccctgga gcttgctgac cgggcctatg
     7981 ttctacagac cggcgccatc gtaatggaag gctcgggggc cgaactcctc aagagcgaca
     8041 tgatccgcaa atcctacctt ggaatgtaaa ggggatattg aatagcttcc caaaggagga
     8101 gggaaatgag agggagcaat aaaaataaca aatctctgtt ttcaaagctt catcacctat
     8161 cagtggttgt gagaaacatg gatgaggcga tcaaattcta tgaatccata gggatcggcc
     8221 cctttgaaga ctatccgcca ttaacagagt atgtaaagct caatgttcag gacgaaaccg
     8281 gattccttaa tttaaaattc aaaatagctc gggtagggga cattcagatt cagttatgcc
     8341 aaccaggtga gggaaagagc ctttacaaag atttcttgga gaagaaggga gaaggagtct
     8401 atcacctagg cttcgtggtg gatgatatcg acgattcgga ggcatcactc aagaagctag
     8461 ggcttagagt tctcagtagt ggaaggcgag tagatgggag tgggttctca tactttgata
     8521 ccgcaggaaa agctggagtc gtgttgctgg ttcgacagag tcccggcgga aagcagaagt
     8581 aagccaagtg aagtcaaccc cgcggctcca aaagatgggg gtacccccct tttttcaaaa
     8641 ctctaaaatg atataatttt gataatttcc taaaaagtct gaatcgacat ttttctgtca
     8701 ttccggcgaa agccggaatc cagtgtctct caaaacttat aattatcctg gactccggtt
     8761 tccaccggag tgacgaattt ttacgaaacc atcaatctca atgttctgat ttctgggaaa
     8821 ccgcggtaaa caaaaaggag atggagcgat gaacgggtat gccggaaaat tgctgagagt
     8881 gaatctttcc aatggaaagg tttcgaagga agagatttcc gattccatga agagagattt
     8941 cttaggtggg cgggggtttg ctgtcaagct cctctgggat gaggtaagaa atgccgaccc
     9001 cctgtccgag aagaacaaga tcgtctttgc cacggggccc ttaaccgggc agtcgattcc
     9061 cagttcagga aagatgatcg ttgcttcgaa gagtcccctt acgggagggt acggggacgg
     9121 aaatatcgga acgatggcct ctgtttacct gaaaaaggcg gggtacgatg tcgtggtggt
     9181 tgaggggaaa tcggagaagc cctgttacct tttgattgag gatgagaagg tcaagatcgt
     9241 cgaggctccc gagctgtggg gcaagaatac ctttgaggcg caagatctcc tggagaagaa
     9301 acatgggaag aattcgggaa tcctcctcac cggacctgcc ggggaaaacc tggtccgtat
     9361 ttcaaccgtg atgtctcaga aaggaagagc gggaggacgg cccggtatgg gtgccgtgat
     9421 ggggtccaag aaactgaagg ctgtcgttat aaaagggacc gggagtgttg gcaacttcga
     9481 cgataacaac ctgcaggaga tgggtaaaaa gggatatgca gagatcaagg ctaaggagag
     9541 ctacgacttc tggatgaagc agggtaccat gcaggccttt gactgggcca atgaaaatgc
     9601 gtgccttcca acctataact acagggaagg cgtctttgag ttttccaagg aaatggatgg
     9661 ctatgtggtt gaaaaactac atgcagggcg caagggctgc ccattgtgta acatgcagtg
     9721 cgggcatatt atcaaggatt cgagcggtca aggcgcagaa ctcgattatg aaaacgtagc
     9781 gatgctcgga ccgaacatcg gcctggggag tctgcccaag gtggcgctgc tgaatcggat
     9841 ggctgacgaa tggggtttag atacgatttc tctgggaagc tgtatcggct ttctcatgga
     9901 ggcttcggag aaaggaatca tcaccgaaaa gataaaatgg ggagactttg aggcagctca
     9961 gaagctgacc atggagattg gaaagggagc ggggcttggg aagctcttct cttacggcgt
    10021 caagggcgcc tccgagaccc tcgggaaagg ctctcaaaag tgggccatgc aggtgaaggg
    10081 gctggaggtc tctgcataca attgtcaggc ctgccctggg atggccctcg ccttcgggac
    10141 ctccccgatt ggagcccacc ataaggatgc ctggatcatc tcttgggaga tcgccaacga
    10201 tcgattctcc tataacaaac caaaagtcga gaagctgatt gagttccaga ggatccgcgg
    10261 gggattcttt gaatcggcaa ccgtgtgcag gcttccctgg gtggaggtca gttttggcct
    10321 cgattggtac cctaaatact ttgaatcgat tacgggagaa aaaaagagct gggacgatat
    10381 ttacaggctt ggagaccgga tctataactt gattcgagcc ttttggataa gagaggtccc
    10441 ttccttcgac cggaaatggg actatccccc tgaacgctgg tttgaggaac cccattcggg
    10501 tggaaagatg aaagggatca aggtcgaaag ggaaaaatac gagcagatgc tctcttggta
    10561 ttatgaactt cggggctggg atcagaatgg aatccctttg aagagaaccc tggatagttt
    10621 gggattaaag ggcgtttcat aggagaccct ggggcaagtt ggctgagctc aagtttttcg
    10681 gctacctggc agacatcgcc ggcgcccggg caaaagagat gtgtttggag aagcccgtgg
    10741 ctttgaggga gattttaccc atctcttttc cggaaacgaa tatcattatc ctcatcaacc
    10801 aggaggtggg ccatctggac agcctgattc gcaatgagga ccgtgtggcc ctcatgcccg
    10861 tcttgtctgg aggataaatt ccccgctgcc cattcaggct gcggaagcag gcaatacgtt
    10921 tcccgaggat caccacttcg ccatggctgg aatttcctat cgaattctgg aagtcgatct
    10981 ttcctccggg caatcgaagg taattgaata tggcgaagag gccctgcgca agtatttcgg
    11041 cggaagcgga ctggccgctt cgatccttct ggaccgcttc gattcttccc tcgttccctt
    11101 tcatccgcaa agcccgttga tcttcatgac cggcctcttt actggaatcc ccgtgccctg
    11161 cgggtgccgc gtttccatct gcgccaaatc gcccctccgg atttgggggg aagccaatgc
    11221 cggcggatac tggggtccgg aattgaaatt tacgggattt gacggcctga tcattaccgg
    11281 gaaagccaaa gagccttctt acctgtgggt gactcccaag gaagcagaaa tcaggagtgc
    11341 gaaggcgctc tggggaaaag gaactttcga aaccacggaa gaactggcga agcaaaccga
    11401 cccgaaggct cgggtagcag ccatcgggcc tgccggagaa agtttaagtt acatggcagg
    11461 catcataacc gggggacttg aagcccgggt catggcccgg agcggcccgg gggcgataat
    11521 ggggtctaag aatctaaagg cggtggtggt gagaggggat caaaaaccat tcatcatcaa
    11581 ccagaagatt ctgcaagagg tcacgaaaga gtttctcctt aatttgagat tcgtggaagg
    11641 gctgagcaaa ttcggcacgg cctcggttat cgaatccaag gaagcggcag gggtcctgcc
    11701 gatcaagaat tttacccagg cccactggga caaggccaag aagatcgccg gcccggcctt
    11761 ggttgccaag tacctggaga gacattacgg ctgttactcc tgccccatcc gttgcggaaa
    11821 agatgtgaag ggcccggagg gaccttatgc gggcgtcgtg ggccacggac cggagtatga
    11881 aaccctgagt gcctttggct ccctgattct gaatgacgac ttgaaatctt tgattcacct
    11941 gaattttctg tgcaatgacc tgggcctgga cagtatgtcc gcaggaatca ccgcggcctt
    12001 tgccatcgaa tccaaccttc ggggctacct caaggaaaat gacggtttgg acctgcaatg
    12061 ggggaacgcc cagtcagtcg gcgtcctcct ggaaaagatc gcccggcggc aggggatcgg
    12121 agccattttc gccgacggtg taaagaaggc ctccagggcc ttggggaaag ggacggaata
    12181 ctttgccctc cattcaaagg gagtagaagt ttccctgtcc aacccgaccc cgacagtgag
    12241 cctggctttg agctgggcca cctccaaccg cggcgcctgc catctcgagg gtttctccca
    12301 ccaggtggag ggaggagttc cattccccga gatgggctat acgcaaggca tggacgggat
    12361 ttccggagag gggaagggca ggatgaccgc cctgatgcag gattacatgg ccgtcttcaa
    12421 tgccctgggg ctttgcaaat tcctgttctt cgcccgggta tctcatgaaa ccctatgcaa
    12481 gtggctgaag ggcttggccg gctgggacct gaccagcaag gatttgatgg aggtgggaga
    12541 gcgcctctac aatttgaagc gggcttacaa cgtcctcatg ggggtcaatg cggagaaaga
    12601 tgtggtggcg ccgaggatat tggagaacct gcgcccggaa aaaccgatca gcgaggggcg
    12661 gaagctattc tttgacatgc gcaaagagta ttatgaggcg cgcggctggg atgaaaaggg
    12721 gattcccacc cgggaaagac tgctctcgct gggcctggaa ggcgctgccg aaaaaattaa
    12781 aagaaaataa aaaccatatg tcccgccaag gcgccaagag cgcagagaat atagatcaga
    12841 gattaaatta aaattagcaa tttttcatga atcttcatcc gtgtttatcc gtgttcatcc
    12901 gtgtcccccc ccaccccaac cctccccctc gaggggggag gggagggagg gggtgatttc
    12961 ttttggttgc ggcacagccg cacttttttc atctgtgtcc aataatatgc ttattcacgt
    13021 caaaaccctc ggcaccttga aaagcctcga agaaggaccg aaggtcattt cccttgaagt
    13081 tccggaaggg gcgcccgtgt ccgatgcgat cgaaaaactg ggaatgaagg actgggaagt
    13141 cggcctcgtt ctcatcaacg gcactcacgc caccaaagag tccattttaa aagaccaaga
    13201 ccaactcacc ctcattgccc ccatcgtggg aggatgaggc gctattttat tcgccggctt
    13261 caaccggatt cccgggtaca aaatcccaga cgatatttct tgacaccctt ccggttggtt
    13321 tctaatattc ttataaggtg atcagcttta tttctcgaag aaaaattccc cgccccctag
    13381 cccttgtgac cctagcaggg ttccttttaa gttgcacgcc ggtccggacc aagaacatcg
    13441 tggaccgggt ctatgaactg gaccctcagg ggaagatctt cgtttccaag actcttctgg
    13501 agaatccccc caagacggtt gcggtccttc cgtttcgcag ccagattggg gagggaagag
    13561 tcgagggaag cgaagcgctg ttcaattctc tcagccggaa ggacccgagc cagccggagt
    13621 cggtgggaga aagaatgcgg cgggccttct tcggccaatt cgctcagctg gaatttgacc
    13681 atgtcaagat ttcccgggtc gaccgaatcc tgcggggcga aggactggat acctgggaaa
    13741 agatgaagtc cctctccccg cagcggttgg gggagctctt aggggccgat gcggttatca
    13801 tcggcgaagt tacccacttc gactactact atgccttttt gtactctcag ctagctgtgg
    13861 gtttgtccct cgagatggtg gacacgagaa ccacagaaat cctttggagg gtccatgatg
    13921 cccggaggga tcacacggtc cgcgttgtct atgacccaat cgcactggtc gtcggactct
    13981 tccaagtggg cttttcgatg cggccaatca acatgatgag agccatggat gagatctgcc
    14041 gggaactggt gggaaccatc cctccgccgc aggagaatca gtaaaaaagc atacggcaca
    14101 gcgcaaaaaa agaaaattcg caaggaagtc ttaatgacaa ggagctaaga tgaaaatcgc
    14161 ttttgttgtt ttcaacggat tgaccactct ggatttcatc ggcatttatg accctctcac
    14221 ccggctaaag accatgggct tcatgccgga cctgggatgg gatatctgtg cctatacgga
    14281 catcgtgaag gatgatcgcg gtctggtcat ggtcccgact gcaattcagg aaccgctcca
    14341 gtcttatgat gtgctggtgg tacccggggg atacggaacc cggaccttat ttcaagacaa
    14401 gaattttatc gagtggataa agaccgctga gccatgtaaa ttgaaagtct cggtctgcac
    14461 cggggcgttg cttttggggg cggcaggttt tctgagagat aaacgggcca ccactcaccg
    14521 gagtgccttt caggaattag agccattctg ccaaaaggtt ctggatcatc gcatcgtcga
    14581 tgaaggaaac atcatcaccg cccggggcgt ttcctcgggg attgatgaag gtctttatgt
    14641 tgtagaaaga ctggcgggtg cggaggcaag atcgaagatt gccagacaga tggactatcc
    14701 ttataccctg caggcagggg atgtggcgaa ataacggagg gaaacggagg gaagagattc
    14761 cttgaccctc cctttctcgt ttgttagaat ccgctcaatg tatccagctt atgggctttg
    14821 aacgaagata ttgagtctga ccaattggac gaggagatat acgtggccgg gattgagtgg
    14881 ctcctggaaa tttatggatg ccctgaagag actctgcgga gccgggaatc cctgaccggg
    14941 cttttccaga cgatcatctc cgcgatggga ttgaagcctc ttggggattc aatttggcac
    15001 cggtttccgg aaacaggcgg attgaccggg ttctggctcc tccaggagtc ccacctggcc
    15061 atccacacct ttcccgaatt ccaatcggca tgcctgaatg ttttttgctg tttcgagcgg
    15121 cctgccattg aatgggaatc gattttgtca gaaaagctgg atgccaggga agtccgggtg
    15181 cgggagtatt tgcgggtcta caaaaaataa gggttccagg gtccggcgcg gcctctgcga
    15241 gagataaatt tttcggatgg attatgaaga aagaggttaa ttgcccttcg tgtggagcga
    15301 aggtcgtctt tcaatccgtg gcctcggtca tggcagtctg ccagtattgc agttccaccc
    15361 tggtcaggca cgatttggac ctggagaaca tcgggaagat ggctcagctc caggccgacg
    15421 gctctccctt gcagttgcgg gcggagggga agtatcaggg ggactccttc acggtcgtgg
    15481 ggagaattca gcttcgcttc gagaagggtt tgtggaacga atggcacctt ctcttcgacg
    15541 accagcgcag cggctggctg ggagaggcgg cgggaatcta cgcggtatcc tttttgactg
    15601 aggtcgggga gaagatcccc caattcgaag aattgcggcc tggggaatcg gtaacgctca
    15661 agaaggcatc ctacgaagtg accaatatgg agaaagcttt ttgcatcggt ggggaaggag
    15721 aacttccttt ccagatcggc cccgggtacg aagcaccggt ggccgacctg gcaggccagg
    15781 gaaagcagtt tgccaccctg gattacagcg aggagcctcc tctggtctat atgggtgagt
    15841 atgtggaatt cgaagaactc catttctccc gattgagaga aattgatgga tggtaggcaa
    15901 ggtcccatgg ctgacccctc cctgacggcg accgctccgg ctcctgccac tccgcaggta
    15961 aagaccttga gatgctcggg ctgtggcaac cccctgaccg tgcgggggat ggagcggacc
    16021 gagtcgatcg cctgcgaagc ctgcgggtcg gtcatcgatc ttaccgatga gaatctgcgg
    16081 atcatctcca cctttcagtc caagatcaaa cacaagccgc tcatccccct gggaagccgg
    16141 ggcaaggtcc ggggggacct tttcgaggtg atcggctacc ttcgccgggc cgttactgtg
    16201 gaaggggtgg attacgagtg gagcgagtac ctcctcttca atccctataa ggggttccgt
    16261 tggctgtccg agtacaacgg ccactggaac tttttgaaga ccaccaccaa catccccaag
    16321 aaaaaggggg atggggtcag atacctggac aagactttcc tccattttca aaccgccgaa
    16381 tccagggtga cgtacgtgct gggggaattt tactggaagg ttcaagctgg ggagacctgc
    16441 aagtatacgg attacgtcgc ccctcccctg atcctgagca aggaacaaac cgcccaggaa
    16501 acagactggt ccatcggaga gtacattgag ccggagaccc tgtggagcgc gtttaagatt
    16561 gcttccccga tgcccgctcg aatcggggtg gcgcccaatc agccgtctcc ctattcaggt
    16621 aaggttgccc gcctatggaa actcatcgga tatttgttcc tggctgcggt gtttatccac
    16681 ttggctcttt tctttttttc ccagaacaag ctggtctttg aaaaccgttt cactttccaa
    16741 cagaaggata aagggaaggc aatcgtaacc gagttttttg atatctccgg gcgcatgtcc
    16801 aatgtggtca taaaaaccgc tgcgtccccg ctggacaaca gctggctttt cctgaatatg
    16861 gccctgatca gcgaagatgg cagggcgtat gatttcggaa gggaaatcag ctattatcat
    16921 gggaccgaag acggcagcgc ctggtccgaa gggagctttt cggatgaagc caccctgccg
    16981 tcgattccgt ccggccggta ttatctccga atcgagccgg aatcacccgc tcccctggtg
    17041 aattaccata tccgggttta ccgggatgtt ccccggtggt cttttttctt tctggctctg
    17101 ggagccctct gcattctgcc gatcttactc atatggcgat tggcgcggtt tgaggggagg
    17161 cgctggtcgg aaagcgacca tccgggtacg ctgtggatgc gcatccccga ttctaccccg
    17221 gaggaggaaa cagggaagga aaaacccaaa ggcaaggtga accgatgaaa aaatttatca
    17281 tcgtgtatgg agccgcagtt ttactattca tggggatcgc cgaataccgc ggctggagcc
    17341 tcatgagcta tcaggagcaa aagggaatcc ccaaatccct gcgggaaaac ccgggtactt
    17401 accggagcag ttacgggggg gtgctacata aataagtttc gagtttcggg ttgcaggttt
    17461 caggtttgct atttcgaatt tttttaactg ggggggaaag atgcttgacc aggtgaaaat
    17521 tggggcgttt atcagttcct tggtgtactc cttcgtgggg gtactggtct tctgggtctc
    17581 ttttatgatc atcgatcggc tgacgcccta tcatctttgg aaggagatca tcgaagagaa
    17641 gaataccgct ttagcgattg tggtgggcgg gatcgccatc ggaatctgca ttatcgtggc
    17701 agccgccatc catggctgag gctttagacc cgcctggacc ctcaggccca tgacctacgc
    17761 cctccttttc tccgttttca tcattgcttc ctgcggctta atctatgagc tgattgccgg
    17821 aacgcttgcc agttatctcc tgggggattc agtcacccag ttctccacgg tcattggaac
    17881 ttaccttttt gccatgggca tcgggtccta cctctcccgc ttcatcggca aggggttggt
    17941 ggccaaattc atcgccatcg aaatcatggt ggggatcatc gggggttttt ccgcaaccgc
    18001 cctgttcctg tctttttcct atgccaagag cctgaagccc cttctgtatt ttttggtgac
    18061 cgtcatcggc atcctggtag ggctcgaaat ccccctcctc ctccggattc tcaaggaccg
    18121 gcttcagttc aaggacctgg tctcccaagt cctcaccttc gattacctcg gagcgctggc
    18181 cgcttccctc ctgtttccca ttttgctggc ccccaggctg ggggtggtgc ggacgtccct
    18241 cctcttcgga ctgatgaacg ccctggtcgc cgccgggaca gcctggcttt tccgctacca
    18301 gctctcccag ctccgataca tattcgttca atgttttctg gccattgccc tcctcggggg
    18361 agggttgatt tacgccgaac gcctgacggc cctggcggaa ggggaactct atgccgatga
    18421 aatcatctac gccaggtcat ccccgtacca aagaatcgta atcacacggt ggaaggatga
    18481 tgtccgcctc ttcctgaaca atcacctgca attcagctcc cgcgacgagt accgctacca
    18541 cgaagccctg gtccaccccg ggttgcagtc cctgaatggg ccgcggcacg tcctgatcct
    18601 cgggggggga gacgggctgg ccgtccggga ggtcttgaaa tacgattcag ttcagaccat
    18661 aaccctcgtg gacctggacc cggagatgac ccgtctgttt tcacggcatg aggttctgac
    18721 ccgcttgaac ctagactccc tcaattcccc caaagtgaag atcatcaatg ccgatgcttt
    18781 tctgtggctg gaagaaaatc aggaattctt cgattttgtg gttgtcgatt ttcccgaccc
    18841 cagcagttat tccctgggca agctctatac cacggctttt taccgccagc tgtaccgctg
    18901 cctttcggag cggggattgg cggtggtcca ggccacctcg cccctttttg cccgccgttc
    18961 gttctggtgt gtcgtcaaaa ccctggagag cgtaggtttc cggacgactc cgtaccacgt
    19021 ttacgtaccg tccttcggcg aatgggggtt cgtgatcgcc tccaaacaac cctttcggaa
    19081 gcctgaccaa tatcttccaa gcctgaaatt tctgaccccc gagctggccc ccagcctctt
    19141 tcatttcccg aaggacatgg ccccggtgga ggcggaggtg aaccgcctga acaaccaggt
    19201 cctggtgcag tactacgagg ccgaatggca caaggtgacc gattagtgaa ccgccggagc
    19261 tttcttcttt catccgcagg acttctttcc g
//
LOCUS       DASPRR010000329         4532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_884445, whole genome shotgun sequence.
ACCESSION   DASPRR010000329 DASPRR010000000
VERSION     DASPRR010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884445"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VEL68_12290"
     CDS             <1..591
                     /locus_tag="VEL68_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ36791.1"
                     /translation="CPCRHRKNLDPNSPDCQHPTENCLHFGTLGRYAVENGMGREITK
                     PEAHKILVEAAASGLVHGLSNWQQAVDTICNCCKCCCMWFESYHKLNHGKSLDSSNYR
                     VKTHPATCKGCGLCVKRCPMDALRLEPSAEAKNKTGKAAVLDPEKCIGCGVCAHKCPT
                     NSLILQRREKTQDPPIDPRDYMQRFFAERKSEKSVS"
     gene            670..1551
                     /locus_tag="VEL68_12295"
     CDS             670..1551
                     /locus_tag="VEL68_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXZ36792.1"
                     /translation="MITIEEQKCTLCGECVPVCVRRILQKGETSVEILDPSMCLYCGH
                     CKAVCPADAFLFSEGNDQFLAVPNREEIPSAAVFFDLLRRRRSLRVYQNRPVEQAKLK
                     MLIEAGRYAPTGSNRQACEYVVLRGRKALDQACTLAIQDLQKQGKQVREAIEQCRRSK
                     KPVPEELLSRETLPPVWERIARRWKEGVDQLLHHAPVLIIVHMKKGLATTPEIDAAIA
                     STQMVLLAETLGLGTCYIAFLIWAIGNSPKLKKMLGIPAENKAQVAFTVGYPAVEFLR
                     FVARSPAKVNWVGEFDN"
     gene            1588..2394
                     /locus_tag="VEL68_12300"
     CDS             1588..2394
                     /locus_tag="VEL68_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ36793.1"
                     /translation="MIDEKRVENIRTYGVHHNTGAPRAEVLFEDLGLAKDRKAEYVII
                     SGCHPPETLPQAFRALKALFDRFRISYTFLSKEYCCGWMPLGQPAVMAKDEAAIARSK
                     ELAQGFILENFKQAEALGAKSIVLFCAACEPTYTNFKKAAHLEVISYSELLERFFRGG
                     KLNREIDYYAGCYRFRRRITQEPIDVAPARRILSKIDGLRVHESDNSLCCFVRPHLDR
                     LAGSFQTKEIVAICTGCYNLLRETLKDREGYRLKMIPELLWEAVEGKDET"
     gene            2597..3475
                     /locus_tag="VEL68_12305"
     CDS             2597..3475
                     /locus_tag="VEL68_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HXZ36794.1"
                     /translation="MKEKQKTKDPGKWVESIIKDFINRSPENTLKNPAKAKAWDDPLV
                     GFSGGDDPIYQAYKEEYVGPFHWTPLELFKMAFPQSKVGANELTIISWVLPQMGPTKA
                     DMRMEWVFPSESWTRARIFGEEVNVKLRKHVVDSLQAAGVEAVAPMLFSEWGMRLSDR
                     FGFASTWSERHAAHAAGLGTFGLCDGLITPRGKAMRAGSVVARAQIPPTPRPYKNHRA
                     YCLFFSHGTCGKCIDRCPIRAISKSGHDKVKCKSFLDMTRNYVLSHHGFEGYGCGFCQ
                     AGVPCESKIPGPEEGK"
     gene            3570..4262
                     /locus_tag="VEL68_12310"
     CDS             3570..4262
                     /locus_tag="VEL68_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ36795.1"
                     /translation="MTRFDRHFVAETKKKLDVDLLGVASIEQSHSTELKKRAEALLPG
                     AKSVVVFGKEVFKELVALLKPGKEVGEAEGGEFFGPHCEYLNGRLTKAVYDLAGTLRK
                     EGYRVFPLPAAGAPMDQRFLVTLLSYKHAAELAGLGTMGRHSMLITPEYGPRVRLAGL
                     LTDAEIEPSSVVKKDSCVNCDACIRACPAGALQTPKEGKAYAINPHACRTYRGTGLTC
                     NICLKACVEVLE"
     gene            4361..>4532
                     /locus_tag="VEL68_12315"
     CDS             4361..>4532
                     /locus_tag="VEL68_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXZ36796.1"
                     /translation="MELAEAIKGRRSIRKFKAQPVPREKLEKILELAMWAPSGMNRQD
                     WYFVVVQGPKREE"
BASE COUNT         1107 a         1196 c         1313 g          916 t
ORIGIN      
        1 tgtccctgca ggcaccggaa aaacctggac cccaactccc ccgactgcca gcacccgaca
       61 gaaaactgcc tgcatttcgg cactctcggg cggtacgccg tggaaaacgg catggggcgg
      121 gagatcacca aaccggaggc ccacaaaatc ctggtagagg ctgcagcctc cggcttggtc
      181 cacggtcttt ccaactggca gcaggccgtg gataccatct gcaactgctg caagtgctgc
      241 tgcatgtggt ttgagagcta ccataagctg aatcatggga agagtctgga ctcttccaat
      301 taccgggtga aaacccatcc cgctacctgc aaggggtgtg gcctgtgcgt gaagcgctgc
      361 cccatggacg ctctccgatt ggagccctcg gcggaagcaa aaaataagac cggcaaggcg
      421 gccgttcttg accccgaaaa atgcatcggt tgcggggtat gcgcccacaa gtgcccgacg
      481 aattccctga ttctccagag aagggaaaaa acccaagacc cgccgatcga tccgcgggac
      541 tacatgcaaa ggtttttcgc cgaaaggaaa tcggaaaagt ccgtcagttg actgtagctg
      601 gtatgagaaa gtatgccccg cgggaaggaa taaataaaaa aatcctacac aaccgagtca
      661 ggagcttcca tgatcaccat cgaagaacaa aaatgcactc tgtgcggcga atgcgtacct
      721 gtctgtgtgc gcagaatttt gcagaaaggg gaaacatcgg ttgagattct cgacccctcc
      781 atgtgtctct actgcggcca ctgtaaggcg gtctgcccgg cggatgcctt ccttttttcc
      841 gaaggaaacg atcagtttct agccgtcccg aaccgggaag aaatcccttc cgcggccgtt
      901 tttttcgatt tgctccggcg acgtcggagc cttcgcgtgt accagaaccg tcccgtggag
      961 caagccaagc tgaaaatgtt gatcgaggca ggtcggtatg cccccacggg gtcaaaccgc
     1021 caggcctgtg aatacgtcgt gctgaggggg aggaaggccc tggatcaggc gtgcaccctg
     1081 gccatccagg acctgcagaa gcagggaaaa caggtccggg aagccatcga gcagtgccgc
     1141 cggtcgaaaa agcctgttcc ggaagaattg ctttcccggg agaccctgcc tccagtctgg
     1201 gaacggatcg ccaggaggtg gaaggaaggg gtcgaccagc ttctccacca cgccccggtt
     1261 ttgatcatcg tccatatgaa aaagggcctg gccacgactc cggagatcga tgcggccatc
     1321 gcctccaccc agatggtcct cctggccgag accttaggac tggggacctg ctatatagca
     1381 tttctcatct gggcgatcgg aaactcgccg aagttgaaga agatgcttgg gatccccgcg
     1441 gaaaacaagg ctcaggtggc tttcacggta ggttacccgg ccgtcgaatt tttgcgcttc
     1501 gtggcccgga gcccggccaa agtcaactgg gtcggtgaat ttgataattg aacccacggt
     1561 tcattcgcgg gagaaaaagg aaaaccgatg atcgacgaaa agcgggtgga aaatatccga
     1621 acctatggag tacaccacaa tacgggggcc ccgagggctg aggttctctt cgaagacctg
     1681 gggctggcca aagaccgaaa ggcagagtat gtcatcataa gcgggtgcca tcccccagag
     1741 acccttcctc aggccttccg ggctctcaag gccctctttg atcgcttccg tatctcctac
     1801 acgttccttt ccaaggaata ctgctgcggc tggatgcctc tggggcagcc agcggtcatg
     1861 gccaaagacg aggcggccat cgccaggtcg aaagaactgg cccagggatt cattcttgag
     1921 aacttcaagc aggccgaggc cctgggagcc aagtccatcg ttcttttctg cgccgcctgc
     1981 gagcctacct acaccaactt caaaaaagcc gcccatctgg aggttatctc ctatagtgag
     2041 cttttggaac gtttctttcg cggcggaaag ctgaacaggg agatcgatta ctatgccggg
     2101 tgctaccggt ttcgccgccg catcacccaa gagccaatcg atgtggcgcc cgcacggagg
     2161 attctgagca aaattgacgg gctgagagtt catgagtccg acaactccct ttgctgtttt
     2221 gtccggccgc atctggatcg cctggcaggt tctttccaaa ccaaagaaat tgtggccatc
     2281 tgcacgggtt gctacaattt gctccgggag accttgaaag atagggaggg atatagattg
     2341 aaaatgattc ccgaactgct ttgggaagcc gttgagggga aggatgaaac ctagccaagg
     2401 atatttgata ttttccttcg attccatcct cctgtgacgg gagggtagag gaagattttc
     2461 acatttaaag gtgtaagcct aataaggatg aaaaggagcc gggtcctgtt cttggaggaa
     2521 tggccaacag aagtggctgg ttaaattgtg aacccgaaac ttgacacctg aaactaatat
     2581 taaaagggag gattggatga aagaaaaaca gaagaccaaa gacccaggga aatgggtgga
     2641 gagcatcatc aaggatttca tcaaccgttc gccggaaaac accctgaaaa atccggcgaa
     2701 ggccaaagct tgggacgacc cgctcgtggg gttctctggg ggagatgatc ccatctacca
     2761 ggcctacaag gaggaatacg taggaccctt ccactggacc cccctggagt tgttcaaaat
     2821 ggcctttccc cagtcgaagg tcggggcgaa tgaactgacg atcatcagct gggtccttcc
     2881 gcagatgggt ccgaccaaag ccgacatgcg aatggagtgg gtgttccctt cggagagctg
     2941 gacccgagcc agaatcttcg gcgaagaagt gaacgtgaaa ctgcgcaagc atgtggtgga
     3001 cagcctccag gcagccggtg ttgaagcggt tgctcccatg ctcttttccg agtggggaat
     3061 gaggctttcg gaccgctttg ggtttgcctc aacttggtcc gagcgacatg ccgcccatgc
     3121 cgccggcctg gggaccttcg gcctctgcga cgggttgatc accccccggg gaaaagcgat
     3181 gcgagccggc tcggtggtgg ccagggccca aattccgcct actccccgcc cttacaagaa
     3241 tcaccgggcc tactgccttt ttttctccca cggaacctgc ggcaaatgca ttgaccggtg
     3301 tcccattcgg gccatctcca aatccggtca tgataaagtg aagtgcaaga gcttcctgga
     3361 tatgacccgc aattatgtcc tctcccatca tggttttgag gggtatggct gcgggttctg
     3421 ccaggctgga gtcccttgcg aatcgaagat tcccggaccg gaagaaggaa aatgaacaat
     3481 gggcaataat gaatgttcaa tgatcatgtt caatgatcca gggtcaatga ctgtctaatg
     3541 aatcatcaat tttggaaagg atatgaaaga tgaccagatt cgatcgccat ttcgtggcgg
     3601 aaacgaagaa aaagctggat gtagacctcc tgggagtggc ttcgattgaa caatcccatt
     3661 cgacggagct gaaaaaaagg gccgaagccc tgttgcccgg ggcaaaatcg gtcgtcgtct
     3721 tcggcaagga ggtattcaag gagctggtgg ccttgctgaa gccaggcaaa gaggtcggag
     3781 aggcagaagg gggagagttc ttcggtcctc attgtgaata cctgaacggg cggttgacca
     3841 aagcggtgta cgacctggcg ggcacgcttc gcaaagaggg ctaccgcgta tttccccttc
     3901 cagccgcggg agcgccaatg gatcagcgtt tcctggtcac ccttctctcg tacaagcatg
     3961 cggccgaact ggccggcctt ggaacgatgg gaaggcacag catgctgatc accccggaat
     4021 acggcccccg agtccgattg gccggcctgc tgaccgatgc ggaaatcgag ccctcttcgg
     4081 ttgtcaaaaa ggattcctgt gtcaactgcg atgcctgcat ccgggcatgt ccggcgggag
     4141 ccttgcagac tccgaaggaa ggaaaggcgt atgccattaa tccccacgcc tgccgaacct
     4201 accgggggac cggcctcacc tgtaacatct gcctgaaagc ctgcgtggag gttctggagt
     4261 aattcattgg gtgccgacgg ccgcagactt tcaggatcac ggaagaatat taaaaattta
     4321 atctgcgaaa atctgcggat caaaaaaatg gaggtcggat atggaattgg ctgaagcgat
     4381 caaggggagg agaagcatac gaaaattcaa ggcccaacca gtgcccaggg aaaaactgga
     4441 aaaaatcctg gagctggcta tgtgggctcc ctcgggaatg aaccggcagg actggtattt
     4501 cgtcgtggtg caggggccga agagggagga ac
//
LOCUS       DASPRR010000330         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2014213, whole genome shotgun sequence.
ACCESSION   DASPRR010000330 DASPRR010000000
VERSION     DASPRR010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014213"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VEL68_12320"
     CDS             <1..547
                     /locus_tag="VEL68_12320"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXZ36797.1"
                     /translation="IIDPLDGTTNYAHGFPVYCVSIALEYGGEIILGVVYDPSREELF
                     VAQKGKGARLNGRRLTVSKTSHLSKSLLATGFPYDLRETPINNFDHFRNFALRTHAVR
                     RCGSAALDLCNVAAGRFDGFWEMKLGAWDLAAGSLIVVEAGGKVTNFEGRPLGLDGSH
                     VLASNRRIHGEMMKILGMGKT"
     gene            complement(438..1850)
                     /locus_tag="VEL68_12325"
     CDS             complement(438..1850)
                     /locus_tag="VEL68_12325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ36798.1"
                     /translation="MKRIAVLSVHSCPLAALGGKETGGMNVYVRELSRQMGRMGIEVD
                     VFTRSQNPHISPIVPLGQNARVIHIKAGPEEPVAKHRLLQYMPEFTSGVVRFARENGA
                     AYDLVHSHYWLSGWVGGRLKKLWAVPLVHMFHTLGILKNTAGEGGREREPSKRLRVER
                     EIGESADCIVAPSPWEKKQMVLLNRTPSARVKVIPCGVDLSLFRPIPSSRAMKVLGLT
                     RRNFILFVGRIDAIKGIDVLIRAVHRLSCRPEGEKGDLGLIIVGGELDADPRRESREM
                     QRLRGLVQDLKLQDRVAFWGSQRQDLLPYFYSAAQALVLPSRYESFGMVALEAMACGT
                     PVIASRVGGLKYTIEDGRTGVLVPEGNSTLLADRVCQVVEGPGVRKRLVHAALAEVRQ
                     FSWPEIAQKVLSVYRSLSSGLGQGKLQGTGRREGWTPEESQSFRSSPSRGFSSSPHEF
                     ACCWPVRGSRPGPKAAPQSS"
     gene            complement(1847..2848)
                     /locus_tag="VEL68_12330"
     CDS             complement(1847..2848)
                     /locus_tag="VEL68_12330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ36799.1"
                     /translation="MGVEIIEGNPPPEKLLPIWEALLQESRFTNPFLTPVWNQLWLKH
                     FGLDLELKTLLLRSSGEEPLGLGIFSHSGKEEGKDHIALLGSKDVWDYRDLVLPAGKE
                     KEALGLLARWAAEGPWGELDFSGISEFSPTMEFLPALLRSSGFQVTWDVEEVAVYLNL
                     PPAWDDFLAQLNSKDRHELRRKMRRLEREAAFEIKEGVGEKLAERLDAFLDLHRKSRK
                     DKSEFMTPLMETYFREIAHRFQERGWLTLPFLRVEGRDIAAYFSFRYRGVEYVFNSGY
                     DPDYSRLSPGIVLAAHCIGQAIEKKLKVFHFLRGQEDYKYRLGGREEKIYRIKAVKG"
     gene            complement(2849..3439)
                     /locus_tag="VEL68_12335"
     CDS             complement(2849..3439)
                     /locus_tag="VEL68_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ36800.1"
                     /translation="MELKRLEVGSFENNCYILICPRTREGVIIDPAAEAEKVLGAVQG
                     ARVKYILITHGHMDHIGALEKVQEGTRATVGIHESDARALRRKPDFFLQDGQDLAAGS
                     FSLKVLHTPGHSPGGVCFFSGKILFSGDTIFPNGPGNTAIPRSDYQEILRSIHSKIFV
                     LPDDTIIYPGHGLETTVGREKSTSFYPLPNDLGRRS"
     gene            complement(3558..>4011)
                     /locus_tag="VEL68_12340"
     CDS             complement(3558..>4011)
                     /locus_tag="VEL68_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXZ36801.1"
                     /translation="SEVKEIEGQVGTNLGDSLLLWLISRWVVGEIPPGHDVIINPVGF
                     AGWIGLLVTSLNLLPVGQLDGGHVVYALVGEKQKKISKIVFAGLLALGIFGWEGWLFW
                     ALLLWIMGFRHPPPLEWWVPLDWKRKVLGWVTLAVFALTFIPVPFSGF"
BASE COUNT          868 a         1217 c         1077 g          849 t
ORIGIN      
        1 gatcatcgat cccctggatg gaaccaccaa ctacgctcac ggattcccag tgtactgcgt
       61 ctccatcgcc ttggaatatg ggggagaaat aatcctgggg gtggtctatg acccctccag
      121 ggaagagctc ttcgtcgccc aaaaggggaa gggggccagg ttgaacgggc gcagattgac
      181 cgtctccaag acttcccatc tctccaaaag ccttctggcc accggatttc cctacgactt
      241 aagggaaacc ccgatcaaca actttgatca cttccggaat ttcgctttac gcacccacgc
      301 cgtgcggagg tgcggctccg cggccctgga cctctgcaat gtggcggctg gacgcttcga
      361 cggcttttgg gagatgaagt tgggggcctg ggacctggcc gcagggagcc tgattgttgt
      421 cgaagcggga gggaaggtca cgaactttga ggggcggcct ttgggcctgg acgggagcca
      481 cgtactggcc agcaacaggc gaattcatgg ggagatgatg aaaatcctcg ggatggggaa
      541 gacctgaagg attggctctc ttcgggggtc caaccttctc ttctccccgt cccttgcagc
      601 tttccttgcc caagccctga ggacaaagac cgatacaccg atagaacctt ctgagcaatc
      661 tccggccagc tgaactgccg aacctcggcc agggcagcgt ggaccagtct ttttctcacc
      721 ccgggtcctt ccaccacctg gcagactcga tcggccagca aggttgaatt gccttcgggg
      781 accagaactc cggtgcgccc gtcttcgatg gtatatttca atcctcccac tcgggatgca
      841 atcaccgggg ttccgcaggc catggcttcc agagccacca tgccgaaaga ctcataccgc
      901 gagggaagga ccagagcctg ggcggcggag taaaagtaag gcagcaggtc ctgacgctga
      961 gacccccaga aggcgacccg gtcttgcaat ttcaaatcct gtaccagacc ccgcaatcgc
     1021 tgcatctccc tgctttccct gcgcgggtcc gcatccagct ctcctccgac gatgatcaac
     1081 cccaggtccc ctttttctcc ctccggcctg caggacagtc tgtggaccgc ccggatgaga
     1141 acatcgatcc ccttgatggc atcgatccgc ccgacgaaga gaataaaatt tcttcgggtc
     1201 agcccgagga ccttcatggc cctggaagaa ggaatgggac ggaagaggct caagtccact
     1261 ccgcagggga tcaccttcac ccgggctgac ggagtcctgt tcaggaggac catttgtttc
     1321 ttctcccagg ggctgggggc tacgatgcaa tcggcagact ccccgatttc cctttcgacc
     1381 ctcagtcgtt tcgaaggttc cctttccctg ccaccttcac cggccgtgtt tttcaatatc
     1441 cccaaagtgt ggaacatgtg aaccagggga acagcccaga gctttttaag ccgccccccc
     1501 acccagccgg aaagccagta atgggagtgg acgagatcgt aggccgctcc gttctccctt
     1561 gcgaagcgga cgactcctga agtgaattcc ggcatgtatt gaaggagcct gtgtttggcc
     1621 accggctctt cgggtccggc cttgatgtgg atgacccggg cattttggcc tagagggaca
     1681 ataggggata tgtggggatt ctgcgaacgg gtgaatacat ccacctcgat tcccatgcgc
     1741 cccatctgcc ggctcagctc ccgcacatag acattcattc ctcccgtttc tttccccccc
     1801 agggcggcca aggggcagct gtggacgctc aaaacggcaa tgcgcttcat cctttcaccg
     1861 ccttgatccg gtagattttt tcttctcttc cccccaggcg gtacttgtag tcttcctgcc
     1921 cgcggaggaa gtgaaaaacc ttcagctttt tttcgatggc ttgcccgatg caatgggcgg
     1981 ccagaacgat ccctggactc aaccggctgt aatccggatc gtaccccgaa ttgaaaacgt
     2041 attcgacccc ccgatagcgg aaggagaaat acgcggcgat atccctcccc tccacccgaa
     2101 ggaaaggaag ggtcagccag cctcgttcct ggaaccggtg ggcgatttcg cggaaatagg
     2161 tttccatcaa gggggtcatg aactcagatt tatcctttcg gcttttccgg tggaggtcca
     2221 aaaaggcatc caatctttcg gccagcttct ctcccacccc ttctttgatt tcaaaggcgg
     2281 cctctctttc caggcggcgc atcttgcggc gaagctcgtg gcggtccttg gagttcagct
     2341 gggccaggaa gtcgtcccaa gcagggggga gattcaagta taccgccact tcttcaacat
     2401 cccatgtcac ctgaaaaccg gaagacctta ggagggcagg caaaaactcc atcgtgggag
     2461 agaactcgga aattccgctg aaatccaact ccccccaggg cccttccgcc gcccaacggg
     2521 ccaaaagacc caaagcctct ttctcctttc ccgcaggaag gaccaggtcc cgatagtccc
     2581 acacatcctt gcttcccagc agggctatat gatctttccc ctcttctttc ccagaatggg
     2641 aaaaaatgcc caagcccagg ggctcttccc cggacgaccg aagaaggagc gttttcagtt
     2701 ccaggtctaa tccaaaatgc ttcagccaga gttgattcca gacgggggtc aggaaggggt
     2761 ttgtaaagcg gctttcctga aggagggctt cccaaatcgg cagcagcttt tccggggggg
     2821 gattcccctc gatgatctcc acacccactc aagacctcct acccaggtca tttggaaggg
     2881 gatagaagga agtcgatttc tcacgcccca cagtcgtctc cagaccgtgg cccgggtaga
     2941 tgatcgtatc gtccgggagg acgaatattt tggagtggat ggaccgcagg atttcctggt
     3001 agtccgaccg ggggatagcc gtgtttcccg gcccattggg aaaaatggtg tcgccgctga
     3061 acaggatttt tccgctaaag aagcaaaccc cgcccgggga atggcccggg gtgtgcagga
     3121 ctttcaggga aaagcttccg gcggccaggt cctgcccgtc ctggagaaag aaatccggtt
     3181 tccggcgtag ggcgcgagcg tcgctttcgt gaatgcccac ggtcgcccga gtcccctcct
     3241 gaactttttc cagcgccccg atgtggtcca tgtgcccgtg agtaatgagg atgtatttga
     3301 cccgggcccc ctggaccgcc cctaggacct tctcggcctc tgcggccgga tcaatgatca
     3361 cgccttcccg ggtgcgcgga cagatcagaa tataacaatt gttctcgaaa gagccgactt
     3421 ccagtctttt caattccacc ttttgctcca agagttgtta ttgctgagtc ctatgtccta
     3481 agaacctatt ttttgcctcc agcctcgagc ggagcggtcc tcgagtcacg ccgaaggcgt
     3541 gacggtcctc caacgattta aaagccgctg aacggcacag ggataaaggt cagggcaaag
     3601 acggccagag tcacccatcc aagcaccttc cgcttccaat ccaggggaac ccaccattcc
     3661 aggggtggag gatggcgaaa gcccatgatc cagagaagaa gcgcccagaa aagccatcct
     3721 tcccaaccga agattcccag ggccagaagc ccggcaaaga cgattttcga tatttttttc
     3781 tgcttctccc caaccagggc atagaccaca tgtcctccat ccagttgacc gacgggaagc
     3841 aggttgaggg aagttacgag aaggccaatc caaccggcaa accccactgg atttatgatc
     3901 acatcgtgcc ccggaggaat ttccccgacc acccaccggc tgatcaacca gaggagaagg
     3961 gaatccccca aattagttcc cacttgtcct tcgatttcct tgacctccga t
//
LOCUS       DASPRR010000331         9629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1202933, whole genome shotgun sequence.
ACCESSION   DASPRR010000331 DASPRR010000000
VERSION     DASPRR010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202933"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VEL68_12345"
     CDS             <1..840
                     /locus_tag="VEL68_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36802.1"
                     /translation="FARQLRTREGTVAQMIVDGTDSNTASVVMTYAQRIISEYSRQIL
                     VQRITSLPNIPDALKRPFFLRGGIDVESRAFFNPNLESRNFYVPGIMAFLIMLVTLLL
                     TCMAIVREREIGTMEQLIVSPIRPFELILGKTLPFALIGYVDVLIFTFVGVFWFEVPI
                     RGSVLLLLAATTIYLLSSLGIGLFISTISHTQQQAMMTMFFFFIPAILLSGFVFPIAN
                     MPPVVQYLTYADPLRYYLVIIRGIFLKGSGFSILWPQMVGLAVVGGIVFTVSSLRFRK
                     RME"
     gene            complement(971..1666)
                     /locus_tag="VEL68_12350"
     CDS             complement(971..1666)
                     /locus_tag="VEL68_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ36803.1"
                     /translation="MTIVDLKREGEVFILTMQGGENRFNPSFIGEMNQALDQVERSTG
                     PAALVTVGGEEKFYSNGLDLGWLMGEGIKEWKNFIPQVLKFLGRVMAFPVPTVAAING
                     HAFAAGAMLALAHDFRVMRADRGFFCLPEVDIKIPLAPGMRALIQSRFSPAVFRDLIL
                     TGARIGGIEAKEKGVVDEAVPLDQVLLRSVARAAALANKDRRTYGNLKRGMYAEALAL
                     LESGEADFSFMSP"
     gene            complement(1847..4435)
                     /locus_tag="VEL68_12355"
     CDS             complement(1847..4435)
                     /locus_tag="VEL68_12355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ36804.1"
                     /translation="MMRSEKPHQQIRPPWHKHPLLILFGIFLILPFLMVRLNSTISMA
                     NEILIFSALMLGFNILLGYTGLLSFGHGAFFGLGAYFCGLSQIHFFKGMVLLPILCGI
                     LGPALVGAVIGFLLMRKRGVYFSLLTFAFTMMFFYIVFRATAVTGGENGLGGIERYPI
                     NLFFFQIDLASQLTFYYFCWVLIVIATVINWRIVHSPFGRVLQAIRENEVRTTCLGYN
                     PKQYKFMAFVLSTFFGGLAGSLYALLLNFAYPQTLHVVTSGEIAAMALVGGMRNFFGP
                     VVGSSIFIFLRDQLSSYTENWLVFFGIIFMGFVLFSYNGIVGILSDLMAYFRKTQDIS
                     PTAPAMIGNPGSSRDPGEEVTGGSPRQSYRKTGDVVFTLQGVCKYFGALAAVDGVSLQ
                     VERGELRSIIGPNGAGKTTLFNVITGLLPIDGGQVHFKDEEVTGLSPDQMVAKGVSRS
                     FQIISIFQDLTVFENLRVAVQARTPYRFNFFSVTEELVDINKEAERIIATVGLNGRED
                     MKASSLSHGDQRLLEIGLSLAVNPELLLLDEPLAGLSSRERTRVGRLIKQLSGEHTIL
                     LIEHDIDRVLTLSDQITVLNQGKVIAEGTPQEIQQNPKVQEAYLGGFHLEEAAPTAAV
                     SAPSAGGLLAVSEINTYYGKSHILHKVSLNVNQGEVICLLGRNGAGKTTTLNSIMGIT
                     PPRNGKISFRGETISGMTSEEIARKGIGLVPQGRRIFPNLTVVENLQIARKEGASQKA
                     WELDRALEMFPKLKELRNRRGETLSGGELQMLAIARTLMGNVEILLLDEPFEGLAPSV
                     VDSVRKTIEEIKGETTILLVEQNASLALSLANRAYVLNNGIIEYAGSAEDLLNNRPLR
                     VRLLGV"
     gene            complement(4432..5316)
                     /locus_tag="VEL68_12360"
     CDS             complement(4432..5316)
                     /locus_tag="VEL68_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36805.1"
                     /translation="MTLTNFFYYFLNGIVMGSLYVLMAIGLSVIFGMIGIINFAHGVV
                     YALGAYAMVLVTQQLGYWPSFVISPILVGLLGMVIEASFLRRIYVGDPLRGLLFTFGL
                     AMVLEQLIRIIWGPSGFPYLIPGYFAGTAKSMASLGIEFSRYRFFIFICAVGIIFGLW
                     WFIEKTPYGMIIRAGSRDPEMVRILGISLKPIMTMVFGLGAALAGFAGVLAAPLAGVQ
                     PSMGDNVGNIAFVVVVIGGLGSFWGAVLSGLLVGEVENLSILIWPPIAQSAMYILMAV
                     ILLLRPRGLFGERWERFE"
     gene            complement(5409..6662)
                     /locus_tag="VEL68_12365"
     CDS             complement(5409..6662)
                     /locus_tag="VEL68_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36806.1"
                     /translation="MRRGKNGITRRSFIKGAGVAALTLGFFGPRGRESLAAEPIKIGQ
                     ITPTTGFLAPTGWWATHGAMLAVDEANAAGGVLGRKIELIMEDEVNPGVAVQKARKLI
                     EKDKVHAIQGTVSSACALAIGDVVQRAKVVFVNTGSNSDEIRNNRCHRYVFSTEGCNT
                     QYVSTIGNWLIKNKQFNRWYFITSDYAFGHDLLRVARNLLKKNNGVELGSELVPTGST
                     DFSSYLLKIKNAKPDTVFHNLAGTDQTNFYKQYKEFGLPFPVAGGVCDTAPAWQAGVG
                     NVLGMFPLVWYHTINKESAAWAEKFLKLYGKLPENQAWCDYVGTQCILGAIAKAGSLE
                     GAKIGQALVGFKIDCLKGRELYFREWDNQLMQPMYVVRGKTREESSDKEWDIFEVVEE
                     IPHKNEPLTAIAIPDSENVCKKEPW"
     gene            complement(6977..8104)
                     /locus_tag="VEL68_12370"
     CDS             complement(6977..8104)
                     /locus_tag="VEL68_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ36807.1"
                     /translation="MNSRERVLTALSHKVPDRVPIDFGSFPGATSMNVWAYKNLLEYL
                     GIKRDVRIGALIMFTAEIDEDILDRFHVDTKTITPSISLSDFGIPEEFVDKPWGVRWQ
                     RSTDYTYAPVEGPFQKIADATAEDLQRYKWPQASEIENFNNWREKARRIRQESDKALV
                     ARVMPGIVTYAQFLRGFENWAVDLALNPEFSRGLHEKLAETWIDAVSLVVKAVEDNVD
                     IVMFGEDLGIQNQTMLSPAMFRERIKPLLKKMVGTIKTRTKAKIAMHTCGSVYAFIDD
                     FIDIGIDVLNPMQSNAKDMGAPKIKGKAGNRLALWGGIDTHEVMPKGTREDVRQEVKR
                     KISIYGKGGGYMLSPDHNVLVDVPPENLMAMFEAAQEYGKY"
     gene            complement(8321..9526)
                     /locus_tag="VEL68_12375"
     CDS             complement(8321..9526)
                     /locus_tag="VEL68_12375"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXZ36808.1"
                     /translation="MTQVKKKKGTIGILTGGGDVPGLNPAIRAVTIRAIREGLQVIGL
                     RNGWKGLISMVRDRKAHQDDHFQILTEEVVNKVGRTGGTFLHTSRTRPSHVSKDDVPE
                     HLQDDYKEKVNDLTPEVLKNLDFLGIDYLIPIGGDDTLSYGVYLAKKGVKVIAIPKTM
                     DNDVPGTDYCIGFSTCITRTIEMVNNLRTTAGSHERILVIEVFGRYAGFTALIPAMAG
                     AANRCVIPEYKFKIDRLTDLLVKDRFTNPSKYSVVIVSEGAMFEGGEMVFMKDEKDPY
                     GHKKLGGIGDLVSDQIKELSGRFNKGKKINTINQRLGYLVRCGNPDAIDSIVPMAFGN
                     LALNLILNHSSGRLVSLRNGRYDDVPIDVVTSVKKVVDVEKYYNTERLRPYYESFESK
                     PLFIMTSDY"
BASE COUNT         2115 a         2757 c         2533 g         2224 t
ORIGIN      
        1 tttgcccgcc agctgcggac gcgcgagggg accgtggccc agatgatcgt ggacggaacg
       61 gactccaaca ccgcctccgt ggtgatgacc tacgcccaga ggatcatctc cgaatactcc
      121 cggcagattc tggtccagcg catcacctcc cttcccaata tccccgacgc cctgaaaagg
      181 cccttcttcc tccgcggcgg aatcgacgtg gagtcccgcg cctttttcaa ccccaacctg
      241 gagagccgca acttctacgt ccccggcatc atggctttcc tgatcatgct ggtcacgctc
      301 ctgctgacct gcatggccat cgtccgggag cgggagatcg gcaccatgga gcagctcatc
      361 gtctctccca tccggccctt cgagctcatc ctgggcaaga cccttccctt cgccctgatc
      421 ggttatgtgg acgtgctcat cttcaccttc gtcggggtct tctggttcga ggtgcccatc
      481 cgcggctcgg tccttctatt actggcggcg accaccattt atctcctcag ttccctggga
      541 atcggcctct tcatctcgac catctcccac acccagcagc aggccatgat gaccatgttc
      601 ttcttcttca tccccgccat cctcctctcc ggcttcgtct tccccatcgc caacatgccg
      661 cccgtcgtcc agtacctcac gtacgccgac cccttgcgct attacctggt catcatccgg
      721 ggaattttcc tgaaagggag cggtttctcc atcctctggc cgcagatggt gggcctggcc
      781 gtcgtcgggg ggattgtctt caccgtgagt tcgctgcggt tcaggaagag gatggagtaa
      841 gaacaggccc gaggtttgag ggtggaggca aaagataaaa ttatgatcat tgagcattga
      901 ccaatgatca atggccaatg aaaagctttt tatgcgacca aacgactatt cgactacgcg
      961 acttttgtgt ttacgggctc ataaagctga aatccgcctc gccgctttcc aggagggcta
     1021 atgcctccgc gtacattcct cgtttgaggt tcccgtaagt ccggcgatct ttgttggcca
     1081 gagctgctgc ccgggcaacg gaccgcagca ggacctgatc caggggaacg gcttcatcga
     1141 ctactccctt ctccttggcc tcgatcccac cgatccgggc gccggtaagt atgagatcgc
     1201 gaaatacagc gggggaaaaa cggctttgaa ttagggctct catgccgggt gccaggggga
     1261 ttttgatgtc cacctcggga aggcagaaaa atccccggtc ggcccgcata acccggaaat
     1321 catgggccag cgctagcatg gccccggcgg caaaggcgtg gccgttgata gccgccaccg
     1381 tgggaactgg gaaggccatg acccgtccaa gaaattttag cacctggggg atgaaatttt
     1441 tccactcctt tattccttcg cccatcagcc agccaaggtc caggccgttg gagtagaatt
     1501 tctcctcccc gcccacggtg accagcgctg ccgggccggt ggaccgttcc acttgatcga
     1561 gcgcctgatt catttcgccg atgaaggatg gattaaaccg gttctcgcct ccctgcatgg
     1621 tcaggatgaa aacctctcct tctcgtttca aatctacgat ggtcataact attttccttc
     1681 ttcctagacg ctcagctacc ccccctccct ccctcttgcc cggaagcggc aggaaagaat
     1741 gagatcaggg aaagggagga gcgagaaaag cgtcggtttc ccataattca ccccggatgc
     1801 ttgataggat catgatacgc ggggaatcga aagaatggct ctccctttaa actcctagca
     1861 ggcgcactct taatggccga ttattcagca ggtcctcggc cgatccagca tattcgatga
     1921 ttccgttatt taacacgtac gctcggttag ccaaagagag ggccaagctg gcattctgtt
     1981 ccaccagtag gatggtagtc tcccccttga tttcttcgat ggtcttccgg acactgtcga
     2041 ccacagaagg ggccaggccc tcaaaaggct catccagaag gaggatctct acattgccca
     2101 tgagcgtcct ggcaatggcg agcatttgaa gttctccccc agacagagtt tccccccgcc
     2161 ggtttctgag ctctttcaat ttgggaaaca tctccaaggc cctatccaat tcccaggctt
     2221 tttggctcgc cccctccttt ctggcgattt gcaggttctc gacgacggtg aggttgggga
     2281 aaatacgccg gccctgggga accaacccga ttcctttgcg ggcaatctct tcggaggtca
     2341 tcccggagat ggtttcgccg cggaaactta tctttccgtt tcgcggcggg gtgattccca
     2401 taatgctgtt caaggttgtt gtcttccctg cgccatttcg gcccaggagg cagatgactt
     2461 cgccctgatt cacgttcagg gataccttgt gaagaatatg acttttcccg tagtaggtat
     2521 tgatctcgga aaccgcgagc agccccccag cagagggtgc ggacaccgcg gccgttggtg
     2581 ccgcttcctc tagatggaac ccacccaggt acgcctcttg aactttcggg ttctgctgta
     2641 tttcttgcgg ggtcccttcg gcgataactt ttccctggtt gagaaccgta atttgatcgg
     2701 aaagggtcag gacgcggtca atgtcgtgct cgatcagaag gatagtatgc tccccggaga
     2761 gttgtttgat gagcctgccc acgcgggtgc gttcgcggga ggatagcccg gccagcggct
     2821 cgtcgagcaa aagcaattcc gggttgaccg ctagggaaag cccgatctcc aggagacgct
     2881 gatcgccatg ggaaaggctg ctggccttca tgtcctcccg accgttcagg ccgacggtag
     2941 caatgatccg ctccgcttcc ttgttgatgt ctactaactc ttccgttacc gagaagaaat
     3001 taaagcggta gggcgtccgg gcttgaaccg ccacccgaag gttctcgaat acggtcagat
     3061 cctggaagat gctgatgatc tggaaagatc gggatactcc tttggccacc atttggtcag
     3121 gactgaggcc ggtgacctct tcgtccttaa aatgtacctg ccctccatca atgggcagga
     3181 gtccggtgat cacgttaaag agggtcgttt tcccggctcc gtttggcccg atgatcgatc
     3241 gtagttcgcc tctctccacc tggagactca ccccgtccac tgctgccagg gccccgaagt
     3301 atttacaaac tccctgcagc gtgaagacca catctcccgt ttttcggtaa gactgccggg
     3361 gggaaccacc cgtaacttcc tctccgggat cccttgaaga tcccgggttt ccgatcatcg
     3421 ctggagcggt tggggatata tcctgcgttt tgcggaagta ggccatgagg tcggagagga
     3481 tccccacgat gccgttgtaa gagaaaagaa cgaaacccat gaagatgatg ccaaagaaga
     3541 ccagccagtt ttccgtatag gagctaagct ggtcccggag gaagatgaag atcgaagacc
     3601 ccaccaccgg gccaaagaaa ttgcgcattc ccccgactaa ggccatcgcc gcgatttcgc
     3661 cggaggtgac cacgtgcagg gtttggggat aggcgaagtt gagaaggaga gcataaaggg
     3721 atccggccag ccccccgaag aaggtggata agacaaaggc catgaattta tactgcttgg
     3781 gattgtagcc caggcaggtg gtgcgaactt cattctcgcg gatagcctgg aggacgcggc
     3841 caaaggggga atgtacaatg cgccagttta taaccgtggc gataacgatc aaaacccaac
     3901 aaaagtagta gaaggtcagc tgactggcca ggtcgatttg aaagaagaaa agattgatcg
     3961 ggtagcgctc gattccccct aacccgttct ctccgccggt gaccgcggtg gcgcggaaga
     4021 cgatgtagaa gaacatcatg gtgaaagcaa aagtcaggag ggagaaatag actccgcgct
     4081 tacgcatcag caggaagccg ataaccgccc ctaccagggc cggccccagg atcccgcaga
     4141 gaatgggcag gaggaccatt cccttgaaga aatgaatctg ggaaaggccg caaaaatagg
     4201 cgcccagccc gaagaaagct ccgtggccga aagagagaag cccggtataa cccaggagaa
     4261 tgttgaatcc cagcattaaa gccgagaaaa tgaggatctc attggccatg gagatcgtgg
     4321 aattcagcct gaccatcaga aacggcaaga taagaaagat accgaagaga ataagcaagg
     4381 ggtgcttatg ccagggggga cggatctgct gatggggttt ttcgctgcgc atcattcgaa
     4441 ccgctcccat ctttctccga ataggccccg gggcctaaga aggaggatga cggccatgag
     4501 gatgtacatg gccgattggg cgatgggtgg ccagatgaga atcgaaagat tttctacctc
     4561 ccctaccaga aggccagaca ataccgcccc ccagaagctt cccaggccgc cgatgactac
     4621 gacgacgaaa gcaatgttac cgacgttatc acccatggac ggctgcaccc cggccagagg
     4681 ggcggcgaga accccggcaa acccggccag ggcggcaccc agtccaaaga ccatggtcat
     4741 gatcggcttg aggctgatcc ccagaatccg gaccatttcc ggatcccggc taccggcgcg
     4801 gatgatcatg ccatacgggg ttttttcgat gaaccaccac aagccaaaga ttatccccac
     4861 cgcacatata aagataaaga agcggtaacg ggagaattca attcccaggg aagccatgga
     4921 ttttgctgta cccgcaaaat atccggggat aagataggga aatccggaag gcccccagat
     4981 gatccggatg agttgctcta gcaccatagc caaaccgaag gtgaagagaa ggccgcggag
     5041 tggatcccca acgtagatgc ggcgcaggaa actagcctcg atgaccatcc ctaaaaggcc
     5101 caccaaaatg ggcgatatca cgaacgaggg ccagtacccc aactgctggg tcaccaggac
     5161 catggcgtaa gcccccagcg cgtacacgac cccgtgggcg aaattgataa tgccgatcat
     5221 cccaaagatg accgaaaggc cgatcgccat gagtacatag agggagccca tgacgatgcc
     5281 attgaggaaa taatagaaga aattcgtcag ggtcataacc ggcttccttt tgaattgtcg
     5341 atggaagtca acatggaaag aagccggccc atcctttagc aggagtgagc cggcccattc
     5401 cggaattttt accagggttc ctttttacat acattttcgg aatccgggat ggcgatggcc
     5461 gtcagcggtt cattcttatg gggaatttct tctacgacct caaagatgtc ccattcctta
     5521 tcggagctct cttccctcgt ctttccccgc acgacgtaca tgggctgcat gagttgatta
     5581 tcccattcgc ggaaatagag ttcccgccct ttgagacagt cgatcttgaa gcccaccaga
     5641 gcctgaccga tcttggctcc ttccaggctg cccgccttgg caatggcccc cagaatgcat
     5701 tgggtaccca cgtaatcgca ccaggcctgg ttttccggta atttcccata gagcttcagg
     5761 aatttttcag cccaggcggc gctttctttg ttgatcgtgt gataccagac aagggggaac
     5821 atgccaagca cattgcccac tccggcctgc cacgcgggtg ctgtatcaca gactccgccg
     5881 gccacaggga aaggcagccc gaattcttta tactgtttgt agaagttggt ctggtccgtg
     5941 ccggccaggt tatggaaaac agtatccggt ttggcattct tgatcttgag gaggtaggag
     6001 gaaaaatcgg tggacccggt gggcacgagt tcgctgccca attccacccc attgttcttc
     6061 ttgagcagat tacgcgctac ccgaagcagg tcatgaccga aggcgtaatc cgaagtgatg
     6121 aagtaccagc gattgaattg tttgttctta atgagccagt tcccaatcgt gctgacgtat
     6181 tgcgtgttac aaccttctgt cgaaaagaca taacggtggc aacggttatt gcgaatctcg
     6241 tcggaattgg aacccgtgtt gacaaagacg actttggcac gctgcactac atctccgatg
     6301 gccagggcac aggcgctgct aacggttcct tgaatagcat gaactttatc tttttcgatg
     6361 agcttgcgcg ccttctgcac cgccaccccg gggttgactt catcctccat gatcagttca
     6421 atcttacgac cgagtactcc tccagccgca ttggcttcat ctaccgccaa catggcacca
     6481 tgagtggccc accaccccgt gggggccaaa aagcctgtgg tgggtgtgat ctggccgatc
     6541 tttatgggct ctgccgccag ggattcccga ccgcgggggc cgaagaaacc cagggttaga
     6601 gcggccaccc ccgccccttt aataaaggac cttctggtta ttccattttt tcctcttctc
     6661 ataatctctc cccctttatt catgattcat tcatggttgg gcattccccc cttatcgata
     6721 attagtcacc ccctttctgc gggcttctct tatgcccggg aaaatctctg gaaatggatt
     6781 gggtcagcgg aacgaatttc aaaaaactgg gaaaaaacat gtgtattttt gtcgaaggaa
     6841 gcttcgctga gggactcgcc cccttgcctc ggatgccagg ttcagtactg ccgtgggatt
     6901 ccggataccc accattattc cttgctcact atgtttgtca agaaatttct tcctgcttct
     6961 tgcccgcccg cagagcctaa tacttcccgt attcctgggc ggcttcgaac atggccatca
     7021 ggttctccgg agggacgtcc acaagaacat tatgatcggg ggagagcatg tatcccccgc
     7081 cctttccgta gatggaaatt ttcctcttca cctcctgccg cacatcctcg cgggtcccct
     7141 tgggcataac ctcgtgggtg tcgattcccc cccacagggc cagcctattg cctgccttgc
     7201 ctttgatctt tggagctccc atgtccttgg cattggactg catgggattc agcacatcga
     7261 tgccgatatc aatgaagtca tcgatgaagg cataaaccga accgcaggtg tgcatggcaa
     7321 tcttagcctt ggtccgcgtt ttgatggttc ccaccatttt tttcagcaga ggcttgatcc
     7381 tctcccgaaa catggccggg ctcagcatgg tttgattttg gatgcccagg tcttccccga
     7441 acatgacgat gtccacgttg tcttccaccg ctttcaccac caagcttacc gcgtcgatcc
     7501 aggtctccgc tagcttttca tggaggcctc tgctgaactc cgggttgagc gccagatcga
     7561 ccgcccagtt ttcaaaccct cgcaggaact gggcataggt cacgattccg ggcatgaccc
     7621 gggctaccaa ggccttatcg ctttcctggc ggatccgccg ggctttttcc cgccagttgt
     7681 tgaaattttc tatctccgac gcctggggcc atttgtatct ttgcaggtct tccgccgtag
     7741 catcggcaat cttttgaaag gggccctcca ccggggcata ggtataatcc gttgacctct
     7801 gccaccggac tccccacggt ttgtctacga actcctccgg tatcccaaaa tcactcaggg
     7861 agatagaggg agtaatggtc ttggtgtcca catgaaatcg gtccaggatg tcctcgtcga
     7921 tctccgccgt gaacatgatc aaggcgccga tgcggacgtc ccgcttgata cctaggtatt
     7981 ccagcagatt cttgtaggcc cagacgttca tggacgtggc cccggggaaa ctgccgaagt
     8041 cgatgggcac ccggtccggt accttgtgac ttaaggcggt gagaactctc tctcggctgt
     8101 tcatggctcc atttcctcct ttcctcaaga aagcaaaatc gatctaaaga aagagggact
     8161 cggcacccct tgccttcctc ttcatccagg ctcattacat taatcaattg ttctcgaatt
     8221 gtaaacagga aatcggtgga aaagagcccc gcgtaggggc gggtttaaga cccgccccta
     8281 tggggtctag gcagggccag tcggctggtc accctacact ttaataatcg ctggtcatga
     8341 tgaacaatgg tttggactcg aagctttcgt aatagggccg aaggcgctcg gtgttgtagt
     8401 atttctccac atccacgacc ttcttcacgc tcgtcaccac gtcaatgggc acatcgtcgt
     8461 acctgccgtt gcgcaggctt acgagccggc cgctggaatg gttcaagatg aggttcagag
     8521 ccaggttccc gaaagccatg ggcacaatcg aatcgatggc atctggattt ccgcaacgga
     8581 ccaggtagcc cagacgctgg ttgatcgtgt taatcttttt ccctttattg aaccgccccg
     8641 acagctcttt gatctgatct gacaccaggt ctccgatgcc cccgagtttt ttatggccgt
     8701 aggggtcttt ctcgtccttc atgaagacca tttctccacc ctcgaacatg gccccctcgg
     8761 atacgatgac caccgaatac ttgcttgggt tggtgaagcg gtctttcacc aggaggtccg
     8821 ttaagcgatc gattttgaac ttgtattccg gaatgacgca tcggttggcc gccccggcca
     8881 tggcaggaat tagcgccgta aagccggcat accgcccgaa tacctcgatc actaggattc
     8941 tctcgtggga acccgccgta gtccggagat tgttgaccat ctcgatggtc cgggtgatgc
     9001 aggtgctgaa cccgatgcaa taatccgtcc ccggaacatc gttgtccatg gttttcggaa
     9061 tggcaatgac cttgactccc tttttggcca ggtacacacc gtagctcagg gtgtcgtcgc
     9121 cgccgatggg aatcaggtag tcaatcccca gaaagtccag gttcttcagg acctcggggg
     9181 tgaggtcgtt gaccttctcc ttgtaatcat cctgcaggtg ttccggcaca tcatccttgg
     9241 aaacgtggct ggggcgggtc cgggaagtat ggagaaaggt cccgccggtc cggcccactt
     9301 tgttaaccac ctcttcggtg agaatctgga aatgatcatc ctggtgggcc ttcctgtccc
     9361 gcaccatgga tatcaatccc ttccagccat tgcggagtcc gatcacctgc aaaccttcgc
     9421 ggatcgcccg gatggtgacc gcccggatcg ccgggttaag cccgggaaca tctcctcccc
     9481 cggtcaggat tccgatggta cctttctttt tcttcacctg tgtcatggcc ggcccccctg
     9541 catctttcca atcggttcga aatcctgcta tgattaattt tatcataaca acataaggca
     9601 aatctaaaaa gaaagactta ggtttgctt
//
LOCUS       DASPRR010000332         4516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2175050, whole genome shotgun sequence.
ACCESSION   DASPRR010000332 DASPRR010000000
VERSION     DASPRR010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175050"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VEL68_12380"
     CDS             <1..446
                     /locus_tag="VEL68_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HXZ36809.1"
                     /translation="LPRGRCCLRDDCACAGKAHFRVCDLELRAMKIKFFPITLGPMRQ
                     LTIRGLRLKEKLETRGIEVFETYPGAAQDIWEIPRQKNPEGLKRGLGRFKVGGNWQGS
                     EVTKDELDALTCALVAREYLRGKTMVIGDPEEGLMVLPRQVRGQR"
     gene            complement(484..1425)
                     /locus_tag="VEL68_12385"
     CDS             complement(484..1425)
                     /locus_tag="VEL68_12385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ36810.1"
                     /translation="MAYDPVERHRKIENLVIQKKVGGDLKKYYRFRHDRWYGGIITAD
                     CAGCGLTCKFCWVRKESILEGREPGEFLSPEEVAQRFLKLMKENKVYQGRISGGEPTI
                     GRSHLFRFLNVFQHKRLKFILETNGILIGEDESYAQELALYPFVHVRVSLKGCTEGEF
                     ARLTGADPGGFHLQLNALRHLKKAGVSVHPAVMLSFSGREAVDQLYSVLWKIDPILHS
                     EIEEERVILYPHVEDKLKREGLKYFSGHTPERKGPGKDDRHEEKEGKRGNGERGKKER
                     PRIVEGGEGSEVPGAKQKEGGQEEPIKGRRIRIITKQ"
     gene            complement(1428..1877)
                     /locus_tag="VEL68_12390"
     CDS             complement(1428..1877)
                     /locus_tag="VEL68_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXZ36811.1"
                     /translation="MLFKKHPKKFFSLEEQDRIVEEIRKAEHRTSGEVRVHLDCCSRE
                     IPLEKAKKVFHRLGMTRTKARNGVLIYVATEDRKFAILGDEGIHRAVPENYWEDVKEK
                     MQKKFREGRICEGICLGIREIGEKLKAYFPVEKDDRNELPDTISEEK"
     gene            complement(1919..2314)
                     /locus_tag="VEL68_12395"
     CDS             complement(1919..2314)
                     /locus_tag="VEL68_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXZ36812.1"
                     /translation="MNADFQDFKCKELTDQIIKIFYRVYNKLGYGFLEKIYENAFMIE
                     LKEEKIVAVAQAPIQVFYKGQILGEYFADILADNKVIIEIRAAKNLMEEHEAQLLNYL
                     KATNIEVGLLLNFGPKPEVRRKAFDNKRK"
     gene            complement(2397..3140)
                     /locus_tag="VEL68_12400"
     CDS             complement(2397..3140)
                     /locus_tag="VEL68_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HXZ36813.1"
                     /translation="MRSLFLAGILCLLYCLSAFGLQVPERPEGRVTDRTGTLTRDQVK
                     ALDQKMASFERETSNQIAVLMIPSLEGDSLEDYSIRLAEKWKIGQEGRNNGVILLIVK
                     NDHKLRIEVGYGLEGALPDALAGTIIRNEIGPRFREGQFYQGIDAGVNAIIAATKGEY
                     KARPIKKSSPIDFWLPFLVVGAFIIFFTLAASQARRRHYHSGGSRGWNSGGGFWYGGS
                     SWGGGGGSDSGGFSGGGGDFGGGGASGDW"
     gene            complement(3167..3736)
                     /locus_tag="VEL68_12405"
     CDS             complement(3167..3736)
                     /locus_tag="VEL68_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXZ36814.1"
                     /translation="MGRMAIIIIPVVIIGVLVIGAFGVYNGLVSSQEEAKTAWAQVEN
                     QYQRRADLIPNLVETVKGFTQQEKSVLEEVTRLRSQWGEARQSGDISKSLQAAQGLDG
                     ALGRLMVVVERYPELKSNQNFMKLQDQLEGTENRISVERKRFNDSVLNYNRKIRTFPG
                     ALFAGLFGFERMPLFEAEQDAKKAPKVKF"
     gene            4156..>4516
                     /gene="rph"
                     /locus_tag="VEL68_12410"
     CDS             4156..>4516
                     /gene="rph"
                     /locus_tag="VEL68_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012685510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXZ36815.1"
                     /translation="MRPDGRSLLQIRPVKMTRNFLKHPEGSVLIELGNTKVICTASVE
                     EDVPRFLKNSEQGWVTSEYSMLPRSTLTRTARESSTGRVGGRTQEIQRLIGRSLRSVV
                     DFTSFGTRTIWIDCDVIQ"
BASE COUNT          972 a         1305 c         1056 g         1183 t
ORIGIN      
        1 ctctgcccag ggggaggtgc tgcttgcggg atgattgcgc ctgcgcggga aaggcccact
       61 tccgggtctg cgacctggaa ctgagggcca tgaagatcaa attcttcccc atcaccctgg
      121 gacccatgcg tcagttgacc atccgcgggc tcagattgaa agaaaagctc gaaacccggg
      181 gcattgaggt tttcgagacc tatccgggcg ccgcccagga tatctgggaa attccgcggc
      241 agaaaaaccc ggagggtttg aagcggggcc tgggccgatt caaggtgggc ggtaactggc
      301 aagggagtga ggttacgaaa gacgaattag acgccctcac atgcgctttg gtagccaggg
      361 aataccttag agggaaaaca atggtgatcg gggacccgga ggaggggctg atggtattgc
      421 ccagacaggt ccgaggtcag aggtgagagg ggaaaaatag gggatcgatc ctattgctat
      481 cgttcattgt ttcgtaatga tccttatcct ccttcccttg atcggttctt cctgaccgcc
      541 ttccttctgc ttcgcacctg gcacctcgct cccctcaccc ccttccacga tcctcggcct
      601 ttccttcttc cccctttcgc cgtttcccct ttttccctct ttctcctcgt gcctatcgtc
      661 tttacccggc cctttccttt ccggtgtgtg tccagaaaaa tatttcaaac cctcacgctt
      721 tagcttatct tccacgtgag gatacaggat gacccgctcc tcttcgatct cgctgtgcag
      781 gatcggatcg atcttccaga gtacgctgta tagctggtcc accgcttccc gtccggaaaa
      841 agaaagcatc accgccgggt ggacgctcac tccggccttt ttcagatgcc gcagggcatt
      901 cagttgcagg tggaaccccc cggggtccgc tccggtcaga cgggcaaatt ctccttcggt
      961 gcaccctttt aaggacaccc ttacatgaac gaagggatag agggccagtt cctgggcgta
     1021 ggattcatct tctccgatca ggattccatt cgtctccagg atgaacttca atctcttgtg
     1081 ttgaaagaca ttgagaaatc ggaaaagatg gctccggccg atggttggtt cccccccgct
     1141 gatccgcccc tggtagacct tgttctcctt catgagcttc aggaatcgct gggccacctc
     1201 ctcggggctc aaaaattctc ccggttcccg gccttccaga atcgattcct tccgtaccca
     1261 gcaaaacttg caggtcaggc cgcaaccggc gcaatcagcg gtgatgatcc ccccatacca
     1321 gcggtcatgc cggaatcggt aatatttttt caaatccccg ccgacttttt tctggatgac
     1381 caggttttcg attttcctgt gtcgttcgac cgggtcgtaa gccaaattta cttctcctcg
     1441 ctgatcgtgt cgggaagctc gttccgatca tccttctcca ccgggaaata ggccttcagt
     1501 ttttctccga tctcccgaat ccccaggcag attccttcac aaattctgcc ttcccggaat
     1561 ttcttttgca ttttttcttt cacgtcttcc cagtaattct ccggaactgc ccggtggatt
     1621 ccctcatccc ccaggatggc gaattttctg tcttccgtcg ccacataaat gaggactccg
     1681 ttgcgggcct tcgtccgcgt cattccgagc cgatggaaaa ccttcttggc tttctccagc
     1741 gggatttccc gggaacagca gtccaggtgg acccggacct cccctgaggt gcggtgctcc
     1801 gccttccgga tctcctctac gatccggtcc tgttcttcga ggctgaaaaa cttcttcgga
     1861 tgctttttaa ataacatggg acgcagatga acgcagataa acgcagatat tcaaagaatt
     1921 atttcctttt attgtcaaaa gctttccgtc tgacttcagg ttttgggccg aaatttagga
     1981 gcaatccgac ttcaatgttc gttgccttta gataattcaa aagttgcgcc tcatgttcct
     2041 ccatcaaatt cttggctgcc cttatttcaa ttatgacttt gttatccgcc aagatatcgg
     2101 cgaaatattc tccgaggatt tggcctttgt aaaatacttg gataggggct tgggcaaccg
     2161 caacgatttt ctcttctttt aactcgatca taaaagcatt ctcataaatt ttctccaaaa
     2221 aaccataacc cagcttatta taaacccgat agaatatttt gataatttga tcggttagtt
     2281 ctttgcattt aaaatcctga aaatccgcgt tcatctgcgt cccatcgatc cgtcagtcaa
     2341 ttctctgtat ttgaaatcct ggaaatctgc gttcatctgc gtcccattcg ttttttttac
     2401 cagtcgccgc tggcaccgcc cccgccgaaa tcgccaccgc cgccggaaaa gccgccgctg
     2461 tcagatcccc ctcctccccc ccaggaagac ccgccgtacc aaaaccctcc cccagagttc
     2521 cacccccggg agccccccga atggtaatgc ctcctcctgg cctgggacgc cgccagggta
     2581 aagaaaatga taaacgcccc cacgaccaaa aaaggaagcc agaaatcgat cggactgctc
     2641 ttctttattg gccttgcttt gtattctcct ttggttgcgg cgatgatggc gttaacccct
     2701 gcgtcgattc cctgatagaa ctgcccttca cgaaaccggg ggccgatttc gttgcgtatg
     2761 atcgtgccgg ctaaagcatc aggaagcgcg ccttccagcc cgtagcccac ttcaatcctg
     2821 agcttgtggt cattcttcac gatgagcaag atgaccccgt tgtttctccc ctcctgcccg
     2881 atcttccatt tctcggccag acggatggaa tagtcttcta ggctgtcacc ttccagggat
     2941 gggatcatta gaacggcgat ctgattgctc gtttcccgtt caaaggaggc cattttctgg
     3001 tctaaagctt tgacctggtc ccgggtgaga gttcccgtgc ggtccgtcac ccttccttca
     3061 ggccgttccg ggacctgaag gccaaatgcg gaaaggcagt aaaggaggca caaaattcct
     3121 gccaggaaaa gactgcgcaa atttcgattt ccgagggccc gctcgatcaa aactttacct
     3181 tcggcgcttt cttggcatcc tgctccgcct cgaagagagg cattctctcg aatccgaaga
     3241 ggccggcgaa gagagccccc gggaaggtgc ggatttttct attataattc aggacgctat
     3301 cattgaaccg cttgcgctcc acgctgatcc ggttttccgt cccttccaac tgatcctgaa
     3361 gcttcatgaa gttctggttg gacttgagtt cgggatacct ttccaccacc accatcaacc
     3421 ttcccaaggc gccgtccagc ccttgcgcag cctgcaaact cttactgata tcccccgact
     3481 ggcgcgcttc tccccactga ctccggaggc gggtgacctc ttccaggacg ctcttttctt
     3541 gctgggtaaa gcctttgacc gtttccacca gattcggaat taagtccgcc ctccgttgat
     3601 actggttttc aacctgggcc caggccgttt tggcctcctc ctgggaactg accagcccat
     3661 tgtaaacccc aaacgcccca atcaccagta ctccaatgat gaccacggga ataataatga
     3721 tggccattct acccatgaga tcctccccgt ctgattttga aaaatgagat tttaatgtag
     3781 cacaggggga gagggaattc aataaagcct ctattcaatt gcggaatgcg gatttcggat
     3841 tgcggaataa aagtccctcc ttacagcctg cgggtgaagg gggctttccc agaccgccct
     3901 aactcatttg acttccccaa gtgaatcgat tattttaagt atattctttt ggctgattga
     3961 aaacgaaaag aatcggatga tttattaggg ggtcattcct gcgaaagtag gaatccagga
     4021 tttttccttt cgccttagaa ttttcttctc cgattaaaag aatttcccat ttttccatga
     4081 atcttaatct acgtttatct gcgttcatct gcgtcccatt aaattatttt gttgtggcta
     4141 aaagaaaggg aaaatatgcg acccgatgga aggtctctcc tgcaaatccg tcccgtcaag
     4201 atgactcgaa atttcctcaa acaccccgaa ggctccgttc tcattgaact gggcaacacc
     4261 aaggtgatct gcacggccag cgtggaagag gacgttcccc ggtttctgaa gaattccgaa
     4321 cagggctggg tgacctccga gtactccatg ctcccccgct ccaccttgac ccgaacagcc
     4381 cgagagtcga gcaccggccg ggtgggaggg cgaacccagg aaattcagcg tttgatcggc
     4441 cggtcccttc gttcggtggt ggatttcacc tctttcggga cacgcacaat ctggattgac
     4501 tgtgacgtca tacagg
//
LOCUS       DASPRR010000333         8906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1856310, whole genome shotgun sequence.
ACCESSION   DASPRR010000333 DASPRR010000000
VERSION     DASPRR010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856310"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VEL68_12415"
     CDS             <1..481
                     /locus_tag="VEL68_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36816.1"
                     /translation="MAFPYFLLAITVIAALGPGLLNGMIAIAIVNIPFFTRIVRGVVL
                     SIKELNYVEAARALGANRYRIIGQHVLINCFSSVVVAGTLNIGWMITAAAGLSFLGLG
                     VQPPTADWGTMIASGRQYISVAPHVATLPGLAIFSTVLGFNLLGDGLRDALDPRLKI"
     gene            complement(784..1491)
                     /locus_tag="VEL68_12420"
     CDS             complement(784..1491)
                     /locus_tag="VEL68_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ36817.1"
                     /translation="MLLQVEKLDFFIGSTQILRSISSKVNKEEIVCLLGRNGAGKSSL
                     IKNIIGLYSCRSGRILFQGKDITAYSPRQKVLAGMGYSPEDAKIFSDLTVEENINLST
                     WIRDPAEVEKKFSFETIFTIFPGIKKFLTRKGLHLSGGEKKMTSIARALALNPSLLLL
                     DEAFEGLAPLVVRHFIEALSQIRELGVAILLGESNVRIASQIAGRSYIVERGEMIFEG
                     HPSEILDNEKLLKIVGR"
     gene            complement(1497..2240)
                     /locus_tag="VEL68_12425"
     CDS             complement(1497..2240)
                     /locus_tag="VEL68_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36818.1"
                     /translation="MLIVKELHKNFGYFQAVNNVSFEIRGGEIVSIIGTNGAGKTTLV
                     NLISGYIKPDSGSIRFSDQDITYLSPYKRVQMGVSRSFQLIQLFEKATVLDNVRIALF
                     SKYGMIRKAFLPADRYSSIAREAVAILDNFHLLDKKDQTPKALSEGDMKVLDVAIAFA
                     LKSKLLLLDEPTSGVATYDKFQVMDSIVSVIRKEGISSLIIEHDMDIVSGYSDRVIVM
                     HEGQILAEGKPDEIMANKDVKAAVFGILS"
     gene            complement(2234..3238)
                     /locus_tag="VEL68_12430"
     CDS             complement(2234..3238)
                     /locus_tag="VEL68_12430"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36819.1"
                     /translation="MELKRMLGNPRWVAYGIILLVLLFVPYLLPTFDLMLFEYGLTFG
                     IVGLGFNLLLGYTGLLSFGHGAYFAAGAYAVAMMGKYLPQAFAMELLIPAALLFSLLV
                     SIIFGFLCVRHTKIFFSILALSLSMLLFALIFKLYHLTGGSDGLRVPIPTILGMSFKG
                     VRRPLFLTGTYYYFLLAMFTLSFLIMRGIVSSPFGKALQAIRDNEIRAELIGIRVRRY
                     RLYAFCISGMYAGLGGALWSFVNGHVTADLSHWVFSGEIVYMTLLGGFGMLEGPVIGA
                     MIFTYLKLYAMGNTEYWMFVIGATLIILVLLLPNGVSGAAAALWTKMRKGRKTEGVSC
                     "
     gene            complement(3242..4108)
                     /locus_tag="VEL68_12435"
     CDS             complement(3242..4108)
                     /locus_tag="VEL68_12435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36820.1"
                     /translation="MWVIYTLNGLYFAALLFLTSLGLNIILGVMGILNLAHGSFYAIG
                     AFMGAWTIIQIVTKAPAPLLYVSLLSGFLLAGIVGWIMEKTLIRAMYKRTLEYQLLLT
                     FGALLLLEDLIKMVWGGQALYASAPFDRLGKVEIMGSVYPAYFLLVIAITLCVGFLIW
                     LFMGKTRLGIMLRAISLDREMATGLGLDISRLSTLAFVLGSGLAGLAGALVIPTTPAL
                     LGIGMEPLIFAFIVVVVGGLGSLKGALVGALLVGLTRSFGIAIFPEIELPLLFLIAGI
                     ILVAKPQGLFGR"
     gene            complement(4168..5490)
                     /locus_tag="VEL68_12440"
     CDS             complement(4168..5490)
                     /locus_tag="VEL68_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36821.1"
                     /translation="MSNEISRRNFLKYVGAATIIVGPLSGATKALGQAKRPAPTGPVK
                     IAAVSFLTGAAAGPFGIPADNTFKMCADVINKEGGILGRKIELLSKDEAGGVDAQVKL
                     ARKLLLEDKVDVDLGYISSASCLAVLPLADELGGLIVAYDCGTHELMEGAKSPYEVPK
                     FKLGFKSSAHLGIDNIGLARMIKKYLPNIKTIAGVNQDYAWGRDSWLIFETAMKKLMP
                     QVQVVKTLWPPLGNTDFTAHISALMGAKPDIVHTSLWGGDAVTFTKQAAGMGFFKNIK
                     TAWSRGEPYPQELGKDFPEEQIICCAGTHYFLYPPPEKSPLNKWFVEEYRKRYGKYPT
                     YPCYHAYQALYLYKHAVEKAAKSAGGWPTQEEIAKAMTGLTFSTPSGDITIREDHSAV
                     EDVLVGFSKLTPKYPFPILDPQRMEIFPANQVLPPVGIRTVDWINGWK"
     gene            6226..7791
                     /locus_tag="VEL68_12445"
     CDS             6226..7791
                     /locus_tag="VEL68_12445"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXZ36822.1"
                     /translation="MAKDEGMQEAIRKLREHRAQAHSVAGKEKIEEVHRKGILTARER
                     VEALLDPGTFVEQYPFAEPTGRDFGLDRKRFPGDGAIVGWGNIEGQRAYVTANDSLVM
                     GGSGSSSHIRKISLTIDQAVKNGSPLIQLNDSSGGRIQEGADKTAYALSVFYSNTLAS
                     GVVPQITAILGRCAGYAVYGAALTDFVFIVEGLGEMFITGPAIIREITGEEITFDKLG
                     GAKVCTQINGAADFLVANEQECFQQIRRLLSFFPSNCRELPQDRANDDPVDRVVSELE
                     DLVPSNPQRAYDIRKVIKQIVDEGDFMETKADFAKNIVTGFARMGGQSIGIVANQPMF
                     LGGSLTVASSDKSARFIRFCDAFNIPLLFLVDTPGYLPGIQQEHTGIIRHGAKLLYAF
                     CESTVPKVAITLRKAYGGGHWAMGGHNAHGTDIAYAWPTAEFAIMGAEQAAKLLYSRE
                     LKAAADPAALLQEKIREYRDLFANPYRLAQTMCIDDVIEPGETRLRLIKAFRSLKGKK
                     EEKPPRRHGNIPL"
     gene            7854..8885
                     /gene="dctP"
                     /locus_tag="VEL68_12450"
     CDS             7854..8885
                     /gene="dctP"
                     /locus_tag="VEL68_12450"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ36823.1"
                     /translation="MKITKKLVFGGLAALIALTSGLTGPVPRAVAQGAKPEFLFRCAG
                     TMPLEHFMTRTLEYYAKIIQERSKGRMKIEIYPVNQLFSDKDLPKALPSGAVDMAQVN
                     MAMWAGLVPSLAIQEIPFFYKDRDHFFRAMISPGVRSVLDKEFEGKGVKILFWMDYGY
                     MAVIGKKPIRNMEEFKGKRIRVFGEISSEFLKALGAAPVFLSVGEVYLALQRGTIDGV
                     LTSTCSVDERKFFEVAKHFTLIKVGEFQNQPAVLVNLKKYQELPPDLQKLVIDASKEA
                     QDWGLDASLKDTDQCLDNVKKKGMEIYYPTEQEKKRWAEATKGILANYLKRTGNVGQA
                     LIEEVNKLR"
BASE COUNT         2159 a         2355 c         2367 g         2025 t
ORIGIN      
        1 tatggccttt ccttatttcc tcctggccat taccgtgatt gccgccttgg ggccggggtt
       61 gcttaacggg atgattgcca ttgccattgt aaatatacct ttctttaccc gcattgtccg
      121 gggagtcgtc ctttccatca aggaattgaa ctatgtcgag gcggctcggg ccctgggagc
      181 caatcgatat cggatcatcg ggcagcatgt tttgatcaac tgcttttcct cggtagtggt
      241 tgcggggact ttaaacatcg gctggatgat caccgcagcc gctggcttga gctttctcgg
      301 attgggagtt caaccgccta ccgccgattg ggggaccatg attgccagcg ggcgccagta
      361 tatttccgtg gctccccatg tggcaaccct gcctggtctg gcgatttttt ccaccgtgtt
      421 gggcttcaat ctcctgggag acggattgcg ggacgcgctg gacccccggc ttaagattta
      481 aaaaatctcc ccggcttccc cgcggattca atcccgaagg gtcagctatt tgggcttgaa
      541 ctggttgatt caaaaaaata gaaaaggggt tccttggagg cttggtaagt cagcgggtcg
      601 attcggtctg tctgcttaac agacctgatt aatgggctga catatacatc gcgatcgcat
      661 cctcgagggt tatctttctt gggtgttgac cagaggccgg ggcactttaa tcgtcgattc
      721 cgccatttga ggcacagcag attcgggaat tccaacggcc cgaagccttc tctggacccg
      781 ggttcacctc ccaacgatct ttaagagctt ttcgttatcc aagatttcac tgggatgccc
      841 ttcgaagatc atctctcccc tttccacgat ataagaccgg ccggcgatct gggaggcaat
      901 gcggacattc gattctccca ggagaatggc tacgcccagt tctctgattt ggctcagagc
      961 ttctatgaag tgccttacca ccaagggcgc caacccttcg aacgcttcat ccaggagcaa
     1021 caaggacgga ttgagggcca atgccctggc gatcgaggtc atcttctttt cccctccgct
     1081 caggtgcagg cccttccggg tcaggaattt cttgatcccc gggaaaatcg tgaagatggt
     1141 ttcgaaactg aatttcttct ccacctccgc agggtctcga atccaagtgg aaaggtttat
     1201 gttctcttcc acggtgaggt cggaaaaaat tttggcatct tcgggggaat atcccattcc
     1261 ggctaggact ttctgccgcg gggaataagc cgtaatgtct tttccctgaa agaggatcct
     1321 tcccgacctg caggagtaaa gcccgatgat atttttgatc agggagctct tcccggcccc
     1381 attccggccc agaaggcaga cgatctcttc cttgtttacc ttcgaggaaa tgctcctcaa
     1441 aatttgggtc gagccgatga aaaaatccaa tttttcaacc tgtaatagca tcttcgctaa
     1501 ctcagaatcc cgaatacagc cgccttcaca tctttgttgg ccatgatttc gtccggcttg
     1561 ccttcggcca gaatttgccc ttcgtgcata acgatgactc ggtccgaata tcccgaaaca
     1621 atgtccatgt catgctcgat gatcaggctg gaaatccctt cctttctgat gaccgaaaca
     1681 atggagtcca tgacttggaa tttatcataa gtggccactc cgctggtggg ttcatccagc
     1741 aggaggagtt tcgatttcaa ggcaaaggca atggccacat ccagaacttt catgtccccc
     1801 tcggagagcg ccttgggtgt ctgatccttc ttatccagca gatggaaatt gtccaggatg
     1861 gcgacggcct cccgggcgat agaagaatag cgatccgccg gcaggaaggc ctttcggatc
     1921 atcccgtatt tggagaaaag ggcaatccgc acgttatcca ggacggtggc tttttcgaag
     1981 agttgaatca gctggaagct ccggctgact cccatttgaa cccttttgta gggggagaga
     2041 taggttatat cctggtcaga gaaccgaatg ctcccgctat ccggttttat atacccgctg
     2101 attaaattga ccaaagtcgt tttcccggcc ccgtttgttc cgataatgga tacaatttcg
     2161 ccccctcgaa tctcaaaact gacgttgttc acggcctgga aatacccgaa attcttgtgc
     2221 agttctttga caatcagcaa gaaactccct ccgtctttct tccttttctc attttcgtcc
     2281 acagggccgc ggcggccccg gaaacgccat tcgggaggag caggaccagg atgatgagcg
     2341 tggccccgat gacgaacatc caatattccg tattccccat ggcgtataat ttcaaataag
     2401 tgaagatcat cgccccgatg actggtcctt ccagcattcc gaaacctccc agtagggtca
     2461 tgtagacgat ctccccggaa aagacccagt gggacagatc ggcggtgaca tggccattga
     2521 caaaagacca gagagccccg cccaacccgg cgtacatgcc ggagatgcag aaggcataaa
     2581 gccggtaccg gcggacccgg atcccgatta gctccgcccg gatctcatta tcccggatgg
     2641 cctggagggc tttgccgaag ggagaactga caatcccccg cattatcaga aagctgaggg
     2701 tgaacatcgc cagcagaaaa tagtagtagg tcccggtgag aaacaggggg cgtcgaactc
     2761 ccttgaagga catgcccaaa atggtgggaa tcggcacccg aagcccatcc gaccctccgg
     2821 tgaggtgata gagcttgaag atcagggcga agaggagcat ggaaagtgag agtgcgagaa
     2881 tggaaaaaaa gatcttcgtg tgccgcacgc agagaaaccc gaagatgatt gagaccagga
     2941 gtgaaaaaag gagggccgcc ggaattaaaa gctccatggc aaaggcttgg ggcaggtatt
     3001 tccccatcat cgccactgca taggctccgg ccgcaaaata ggctccgtga ccgaaagaca
     3061 ggaggcccgt gtatcctagg aggaggttga aaccaagtcc tacaatgccg aaggttaacc
     3121 cgtattcgaa gagcataagg tcaaaagtgg gaagaagata ggggacgaat aataaaacca
     3181 ggaggatgat cccgtaggca acccatctcg gatttccaag cattctcttt aattccacat
     3241 tctatctccc gaacagtccc tgcggcttgg ccaccaggat gatccccgcg atgaggaata
     3301 aaaggggcaa ttcgatctcc ggaaatatgg caatgccgaa ggacctggta agtcccacaa
     3361 gcagagcccc caccagggcc cctttgaggc ttcccagtcc acctacaacg accacaataa
     3421 aggcgaatat gagcggttcc atcccaattc ccagcagcgc cggagttgtg gggatgacca
     3481 aggctccggc caacccagcc aatccagacc ccaaaacgaa agccaaggtg ctgagtctgc
     3541 tgatatccag tcccaacccc gtagccattt ctctgtccaa ggagatggct cttagcatga
     3601 ttcccaacct ggttttcccc ataaacagcc agatcaggaa cccgacgcac agggtaatcg
     3661 cgatcaccaa taaaaaataa gccgggtaga cgcttcccat aatctctacc ttgcccaaac
     3721 gatcgaaagg agccgaggcg taaagggcct gcccccccca gaccatcttg atcaaatctt
     3781 ccagtaggag gagcgctccg aaggtaagca agagctgata ctccagcgtt cgcttataca
     3841 tggcccggat caaggtcttt tccatgatcc agcccacgat tcccgcaagc agaaacccgc
     3901 tcaggagaga tacatagagg agaggtgccg gggccttggt cacgatttga ataatcgtcc
     3961 aggcccccat gaacgcaccg atggcataaa aagaaccatg agccaggtta aggattccca
     4021 tgacgcccaa tatgatatta agacccaggg aggttagaaa taggagggcc gcaaaatata
     4081 gaccattgag cgtgtaaatc acccacatcg ccgattctct cccaagatta tcccctcccc
     4141 cggaccggag gaggggaaag gacctggcta tttccaacca ttgatccaat caacggttcg
     4201 tatgcccacg ggcgggagta cttgattggc tgggaagatt tccatccttt ggggatccag
     4261 gatggggaaa ggatatttcg gggtgagttt ggaaaatcca acgagcacat cctcgacggc
     4321 gctgtggtct tctcttatgg ttatatcccc tgaaggcgtg gaaaaggtca ggccggtcat
     4381 ggctttggca atctcctctt gggtcggcca accccccgct gatttggccg ctttctcgac
     4441 ggcatgttta tagaggtaca atgcttgata agcatgataa caggggtagg tgggatattt
     4501 tccatacctc ttccggtact cttccacgaa ccatttgttg agaggtgatt tctcgggcgg
     4561 cgggtagagg aagtaatggg ttccggcaca acagataatc tgctcttcgg gaaaatcttt
     4621 tcctaattcc tggggatagg gttccccccg cgaccaagcg gtcttgatat ttttaaaaaa
     4681 tcccattccg gcagcctgtt tggtaaaggt gacggcatcc ccgccccata aggaggtatg
     4741 gacgatgtcc ggcttggctc ccatcagggc gctgatgtgg gcagtgaaat ccgtatttcc
     4801 cagaggaggc cacagggttt tcacgacctg tacctggggc atcagctttt tcatggcggt
     4861 ttcaaagatc agccagctat ccctccccca ggcataatct tgattgaccc ccgcgatggt
     4921 tttaatattg ggcaaatatt tcttgatcat ccgggccagc ccaatattat cgatgcccaa
     4981 atgggccgag cttttgaaac ctaatttgaa ctttggaact tcataaggag atttcgcgcc
     5041 ttccattaat tcatgagtcc cgcagtcata ggctacgatc aaccctccca gttcgtccgc
     5101 cagggggagg acggccagac aggaggccga agaaatatag cccaggtcca catcgacctt
     5161 gtcttccaga agcaacttcc gggcaagttt aacctgagcg tccacgccgc ccgcttcgtc
     5221 tttgctgagt aattcgatct tccgccccaa tatcccccct tctttattga tcacatcggc
     5281 acacatcttg aaagtattgt cggcggggat cccaaaagga cctgcggctg cgccggttaa
     5341 aaaacttacc gcggctattt ttaccggtcc ggtaggggcc ggtcttttgg cttgacccaa
     5401 agccttcgtg gctccgctga ggggtccaac gattattgtg gccgctccta catattttaa
     5461 aaaatttcgc ctggaaatct cattggacat agatcatctc cttgatgcga gaaaagttaa
     5521 aaaagcaggg ttggtatctc ctccggccaa gcgttcttga tggatggaag cttcaccccc
     5581 ttcctgcctt tgtcggctcc cctttccccc acggacgaca ggaattcgat tggcaattta
     5641 ccgaaagctt acaagaggga attttgagcc gatttttggc cttcctaaaa tgtttcgggt
     5701 ttgaagcggc agtactcaaa ggaactggaa acaatgaata tgctgcaaac ggcttttgtt
     5761 aacttatcag aaaaaatagt ctttcagaaa gaggattgtg tctgctttag gggtctacct
     5821 gcgctccgat cgagagttcc cataaggcta ccgcaaacag tcagaccaga gcgcggtcgc
     5881 aggggaaaaa cgaaaatagt cgttttagaa ttcagggcgg attatattga atgggaaaaa
     5941 taatgtcaat atttttttgg gcagattttc atctcctggg cctcttctga tgttgtgtgt
     6001 atgggagcaa gccgggtttg gccttctctg gcccagattt tggttccgag aggtaaagag
     6061 tggggttcct tccaatcgct ccgaatctct tgacacgggt ttaggcctgt gttacgtgaa
     6121 cagatgaacc cgggaagtcg ggaaaaataa aacccctttt ttaatttttt tttgatggca
     6181 cgaacttatg gatcaaggca ttgaataagt gcaggaggtt tgaatttggc caaggatgaa
     6241 ggaatgcagg aagcgattcg gaaattgcgg gaacacaggg ctcaggccca ttcagttgcc
     6301 gggaaagaaa aaatcgagga agtccaccgg aaagggatct tgaccgcccg ggagcgggtc
     6361 gaggccctcc tcgacccggg cacattcgtg gaacagtacc ctttcgccga gcccaccggc
     6421 cgggatttcg gcctggaccg caagcgtttt ccgggagacg gggcgattgt gggctggggg
     6481 aacatcgagg ggcagagggc atacgtgacg gccaatgaca gcttggtcat gggtgggtcg
     6541 gggtccagtt ctcatattcg caaaatttca ttgaccattg atcaggcagt gaaaaacggc
     6601 tcgccattga tccagctcaa cgactcatcc ggggggagaa tccaggaggg ggctgacaag
     6661 accgcctacg cgctttccgt cttttacagc aacaccttgg cctccggggt tgttccccag
     6721 atcaccgcca tcctgggccg ttgcgccggt tacgcggtct acggcgcagc cctgaccgat
     6781 tttgtcttca tcgtcgaagg cctgggcgag atgtttatca ccggcccggc catcatccgg
     6841 gaaatcaccg gggaggagat cacctttgac aagctcggcg gagcgaaagt ctgcacccag
     6901 atcaatgggg cagcggattt tctggtcgcc aacgagcagg aatgtttcca gcagatccga
     6961 aggctgttga gttttttccc ctccaactgc cgggagttgc cccaggaccg cgccaatgac
     7021 gaccccgtgg accgcgtggt ttccgaattg gaggatttgg tcccctccaa tcctcagcgc
     7081 gcctacgaca tccggaaagt catcaagcaa atcgtggacg aaggcgactt catggaaacc
     7141 aaggccgact ttgccaagaa tatagtcacc ggtttcgccc gaatgggggg acaaagcatc
     7201 gggatcgtcg ccaaccagcc catgttcctg ggcggctcac tgactgtcgc ttcctcggac
     7261 aagagtgccc ggttcattcg tttctgcgac gccttcaata tcccgttgct ttttctggtg
     7321 gatacccccg ggtatctccc gggaatccag caggagcata cagggatcat ccggcatgga
     7381 gccaaactcc tctatgcttt ctgcgagtcg accgtcccta aggttgccat caccctccgc
     7441 aaggcctacg ggggaggaca ttgggccatg ggcggccata acgcccatgg aaccgatatc
     7501 gcctatgcct ggcccactgc tgagttcgcc atcatgggtg cggaacaagc agccaagctt
     7561 ctatacagcc gtgagttgaa ggcggcggcg gacccggcgg ctctcctcca ggagaaaatc
     7621 cgcgagtacc gggacctttt cgccaacccc taccgcctgg cccagaccat gtgcatcgac
     7681 gatgtgatcg aacccggcga gacgcgtctc cgcctgatca aggctttccg ttccctgaag
     7741 ggcaagaaag aggaaaaacc gcctcgccgg catggaaaca tcccgctata gaaaaaattc
     7801 gcgcgggtaa agatatgatt gcccagaaaa aaactcaacc gaaaggagac aacatgaaaa
     7861 tcacgaaaaa gctggttttt gggggtctcg cagcgctgat cgcactgacc tcaggtttga
     7921 ccgggcctgt accccgagct gtagcgcagg gagccaagcc cgaatttctt tttcgatgtg
     7981 caggaaccat gcccctggaa cattttatga cccggacgct ggaatattat gccaagatca
     8041 tccaggagag gtccaaaggc cggatgaaga ttgaaatcta tccggtcaac cagcttttca
     8101 gcgataaaga tttacccaaa gccttgccct ccggtgcagt ggacatggcc caggtaaaca
     8161 tggccatgtg ggccggattg gttccctcgc tcgccatcca ggagatcccc ttcttctaca
     8221 aggaccggga tcattttttc cgggccatga tttccccggg cgttcgaagc gtgctggata
     8281 aagaatttga gggtaaaggg gtgaaaatcc ttttctggat ggattatgga tacatggccg
     8341 tgatcggcaa gaaacccatc cggaacatgg aggagttcaa aggaaagcga atccgggtct
     8401 tcggggaaat ctccagcgaa ttcctcaagg cgttgggagc ggcgccggtt ttcctgagcg
     8461 tcggggaggt ctatctggct ctccagaggg ggacgattga tggggtcctc acctccacct
     8521 gttcggtgga cgaaaggaag ttcttcgaag tggccaaaca tttcaccttg atcaaagtgg
     8581 gggaatttca gaaccagcct gcagtgctgg tgaatttgaa gaagtatcag gaactcccgc
     8641 cggacctgca aaaactggtg atcgacgcct ccaaggaagc gcaggactgg gggctggacg
     8701 cctccctgaa ggataccgat cagtgcctgg acaacgtcaa aaagaaggga atggaaatct
     8761 attatccaac cgaacaagag aaaaagagat gggccgaagc caccaagggc atcctggcca
     8821 attatttgaa gcgtaccgga aacgtgggcc aggctctcat tgaagaagtg aacaagctgc
     8881 gatagggtcg cctcccggaa atgaat
//
LOCUS       DASPRR010000334         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_729280, whole genome shotgun sequence.
ACCESSION   DASPRR010000334 DASPRR010000000
VERSION     DASPRR010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729280"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VEL68_12455"
     CDS             <1..547
                     /locus_tag="VEL68_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36824.1"
                     /translation="LFYVKLGWLPGGGRIADGIQVERISGFLILDSLLTGNWEALGSA
                     LLHLVLPAFCLSSSFLATISRIARSSVLQVIREDYVRTARAKGVPEFLVMAKHVLRNS
                     LLPVITISGILYGQLLGGAVLTETIFSWPGMGRYVVRSILHLDFQPILGFTVLVAILY
                     AVANLVVDLLYSVVNPQIRLE"
     gene            554..1390
                     /gene="nikC"
                     /locus_tag="VEL68_12460"
     CDS             554..1390
                     /gene="nikC"
                     /locus_tag="VEL68_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HXZ36825.1"
                     /translation="MKRAVRHFFFNPLSLLGILFIGVLLFTGILAPLLAPYDPIEISP
                     DRRLTPPNSTNWLGTDEVGRDILSRIIHGARISLEIGVTIVFFAAVIGLVIGLVSGFS
                     GGILDQTLMRFTDMFMSFPTLILAIAMTAALGPSLFNAVLAMIIVWWPIYARLIRGEV
                     LAVKEKEYIRAIRALGARPFKIIFFHVLPNTIDVVVIRASIDFGNAVMFCAALSFIGL
                     GAQPPQPEWGAMVTTGRDYLRDAWWLVTFPGLAIFLTVMGFNLFGDSLRDFLDPKLRG
                     ER"
     gene            1423..2571
                     /locus_tag="VEL68_12465"
     CDS             1423..2571
                     /locus_tag="VEL68_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ36826.1"
                     /translation="MKISDIKVHYLEIPFPRELHSTWGHGRAEKFHGVALTEVQTDEG
                     ISGYGAAEAMWGWGAVHKAVIESMLKPRLLGKDPFATEQHIMDIRDVPGRAWMVENAL
                     WDIIGKVAKMPIYKMWGGFQNRVRAYAAWAELRTPEKAAEDAKFLVEKGIKGVKLRLH
                     HPNMAEDLALVKAVRKSIGDKLTIMVDANQATAPYRTGALRATSLWNFERAHKMARAL
                     EDLDVYWLEEPLPLHNYRDLTRLAAAVDILIAGGEINREIFEFKAFLDQECYDILQPN
                     CTMAEGMFQVRKIAALAEVNHKLCVPHAWVQGPGFYANLHVAGAVRNCPWIEFAFDPP
                     VLTPENFHCLITEPPMIDAEGYVPLPEKPGLGVEINWDVVKRFKKEVG"
     gene            2670..>3725
                     /locus_tag="VEL68_12470"
     CDS             2670..>3725
                     /locus_tag="VEL68_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ36827.1"
                     /translation="MKITKIEIIPVNIPFTRTLKTAIGDWSLAEFVIVRMHTDAGIVG
                     LGEVPPWMAVSRTSQGAILQILKDYICPVILGLDPFQVEKAWDLMDLHAPGNPMAGTP
                     IDMAMYDIMGKALKTPVYNLLGGKFRDKVPTAAIIGFGDTKAMVKECKEWLDLGVKAV
                     RIKVGLGLAKDMKNTEAIRKTIGPDVTLRVDCNQAFTRNEAIRVLQALEPFELELAEQ
                     PVAWHDLAGMGMVARKVRTPIMAHETLLDRFDALHLIEYGGAGVFGLKLDRPGGFVNV
                     RRVNALAELNNIPCFMCSSIELGISTSAAAHLAVSLKNMKLASEFSGPQTISDDIVKN
                     PIPIKDGYTKPWDAPGLG"
BASE COUNT          742 a         1102 c         1077 g          804 t
ORIGIN      
        1 tctcttctat gtcaagctgg gctggcttcc gggcggcggc cgaattgccg acgggatcca
       61 ggtggagcgt atctccggct ttttgatcct ggatagtctc ttgaccggaa actgggaggc
      121 tctgggttcc gcgctgctcc acctggtttt gccggccttc tgcctctcca gctccttcct
      181 ggcaaccatt tcccgcattg cccgctcctc ggtcctgcag gtgatccggg aagattatgt
      241 ccggaccgcc cgggccaagg gggttccgga attcctggtc atggctaagc acgttctgcg
      301 gaattccctt ctgccggtga tcactatttc cgggattctc tacggccaac tcctgggcgg
      361 tgcggtgttg accgaaacga tcttctcctg gccgggaatg ggccgatacg tggtgcgctc
      421 cattctacac ctcgatttcc agcccatcct gggatttacg gtcctagtgg cgattctcta
      481 tgccgtggcc aatctagtgg ttgacctgct gtattcggtg gtgaacccgc agattcgcct
      541 ggaatgaaga cttatgaagc gagcagtaag acattttttc tttaatcccc tttcccttct
      601 ggggattctg tttatcgggg tcctgctctt caccggtatc ctggctcccc tcctggcgcc
      661 ctatgatccc atagagattt cccccgacag acgcctgacc cctcccaatt ccaccaactg
      721 gctgggaacc gatgaggtgg gccgcgacat cctgagccgc atcatccatg gcgcccgcat
      781 ctctcttgag atcggagtaa cgattgtgtt cttcgccgcg gtcattgggt tggtgatcgg
      841 cctggtcagt ggattttccg gtggaatcct ggatcagact ctcatgcgct tcacggatat
      901 gttcatgtcg ttccccacgt tgattctggc catcgccatg accgcggccc tgggacccag
      961 cctgttcaac gcggttttgg ccatgatcat cgtctggtgg cccatctacg cccgcctgat
     1021 ccgcggcgag gtcctggcgg tgaaggaaaa ggaatacatc cgggccatta gagccctggg
     1081 ggcgaggccc ttcaaaatta ttttcttcca cgtcctaccc aataccatcg atgtagtcgt
     1141 gatccgcgct tccatcgatt ttgggaacgc ggtcatgttc tgcgcggctt tgagtttcat
     1201 cggtctgggt gcgcagcctc cccagccgga atggggagcc atggttacga ccggacgaga
     1261 ttacctgcgc gatgcctggt ggctagtgac tttcccgggc ctggctattt ttcttaccgt
     1321 catgggattt aatctctttg gggacagcct ccgcgacttt ctcgacccaa agctgcgggg
     1381 ggaaagataa agaatagtgg ttaaatggta ttgggggttt ttatgaagat tagcgatatc
     1441 aaagtccatt acctggaaat tccctttccc cgggagcttc attccacctg gggccacggc
     1501 cgggcggaga aattccacgg ggtggctctg acggaggtcc agaccgacga gggaatctcc
     1561 gggtacggag ctgcggaggc catgtggggc tggggcgcag tacacaaggc ggtcatcgaa
     1621 agcatgctca agccccgcct gctgggaaaa gacccttttg ccactgaaca gcacatcatg
     1681 gacatccggg acgtgcccgg gcgggcctgg atggtggaga acgccctttg ggatatcatc
     1741 ggcaaggtgg ccaagatgcc catctacaag atgtggggtg ggtttcaaaa ccgggtacgg
     1801 gcatacgctg cctgggctga gttgcgcacg ccggaaaaag ccgcagagga cgccaaattt
     1861 ctggtggaaa agggaattaa aggagtcaaa ctccgcctgc accaccccaa catggccgag
     1921 gacctggcct tggtcaaagc ggttcgaaaa tcgatcgggg acaagctgac catcatggtg
     1981 gatgccaacc aggccacagc cccttaccgc accggcgcgc tgagagccac ctccctctgg
     2041 aattttgaac gggcccacaa gatggcccgg gccctggagg accttgatgt atactggctg
     2101 gaagagccgc tgcccctgca taattaccga gacttgactc gactcgcagc cgcggtggac
     2161 attctgatcg ccggcggcga gatcaaccgg gaaatctttg aattcaaggc cttcctggac
     2221 caggaatgct acgacatcct ccagccgaat tgcacgatgg ccgaggggat gttccaggtg
     2281 cgcaagatcg ccgccctggc agaggtcaac cacaagctct gcgttcccca cgcctgggtg
     2341 caaggtcccg ggttctatgc caacctgcat gtggcggggg ctgttcgcaa ctgtccttgg
     2401 atcgagttcg cctttgaccc gcctgtcctc accccggaaa atttccattg cctcatcacc
     2461 gagcccccga tgatcgacgc cgagggctac gtgccccttc cggaaaagcc cgggttgggt
     2521 gtagaaatca actgggatgt ggtaaagcgg ttcaaaaagg aggtcggcta gtcgttccga
     2581 agggatgcct cgtctttccc ttatttcctg caattctgcg ttctctgcgc tctctgtggt
     2641 gaaaaaacca ataaaatgct ggaggaaaaa tgaaaatcac caaaattgaa atcatccccg
     2701 taaacatccc cttcacccgg accctcaaga cagccatcgg ggactggagc ctggccgaat
     2761 tcgtcatcgt gcgcatgcac accgacgcgg ggatcgtagg gctgggagaa gttcccccct
     2821 ggatggccgt ttcccgcact tcccaggggg cgatcctcca gattctcaag gactacatct
     2881 gccccgtgat tctcgggctg gacccgttcc aggtggagaa ggcctgggac ctgatggacc
     2941 tccatgctcc gggaaacccc atggccggga cccccatcga catggccatg tacgacatca
     3001 tgggcaaggc cctgaagacc ccagtctaca acctcctggg gggaaaattc cgggataagg
     3061 tccccactgc cgcaatcatc ggtttcggcg acaccaaagc catggtcaag gagtgcaagg
     3121 aatggctcga cctgggcgtc aaggccgtgc gcatcaaggt cgggctcggg ctggccaagg
     3181 atatgaagaa cactgaagcc atccgaaaga ccatcgggcc ggacgtgacc ctccgcgtgg
     3241 actgcaacca ggcgttcacc cgcaatgaag cgatccgggt gctccaggcc ctggagccgt
     3301 tcgagctgga gctggccgaa cagccggtgg cctggcacga cctggccggc atgggaatgg
     3361 tggcccgcaa agtgcgcacc ccgatcatgg cccacgagac cctgctcgac cgtttcgacg
     3421 cccttcacct gatcgagtac ggcggggccg gggtcttcgg cctgaagctg gaccggcccg
     3481 gtgggtttgt caacgtgagg agagtgaacg ccctggcgga attgaacaac atcccctgct
     3541 tcatgtgctc gtccatcgag ctcgggatct ccacttcagc cgcggcgcac ctggcggtct
     3601 ctctgaagaa catgaaactg gccagcgaat tctccggccc ccagaccatt tccgatgata
     3661 tcgtgaaaaa cccgattccg atcaaggatg gctataccaa accctgggat gctcctggtt
     3721 tagga
//
LOCUS       DASPRR010000335         5104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2175681, whole genome shotgun sequence.
ACCESSION   DASPRR010000335 DASPRR010000000
VERSION     DASPRR010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175681"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1210
                     /locus_tag="VEL68_12475"
     CDS             248..1210
                     /locus_tag="VEL68_12475"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXZ36828.1"
                     /translation="MLPSCKDPFKVVRRSKLFIPVNREKFVAKAWTRGADCLILDLED
                     SIPPAEKSSARRMVKDVIPLVKKGGAEIEVRINRDYEEEDLAAVVGPEVASLMIPKCE
                     SAEELRRLDRRVAQLEKERGVPEGRTDFSLLIETARGVVRMEEIVAASPRIVQASIGG
                     VDLTQDMGFTRVRDLLAEQFSYPAGKLLYAARAAGVQAAGLWPQNNVDFTDTTLSYEA
                     MLHACRRSFQMGYLGSSTIHPAWVRPLNEGFKPSASELDWARRVKVALEEAYSQGLGS
                     VAVDGRMVDVANLKQVQKILERAEAIDRREAEKETAMAATGGRA"
     gene            1426..2379
                     /locus_tag="VEL68_12480"
     CDS             1426..2379
                     /locus_tag="VEL68_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36829.1"
                     /translation="MRQYILKRILLMVPTLLGVAVLIFLLMRVVPGDLVELKHAGEGS
                     ALSAETLQKERELLGLDKPLWYQFGVWVWDILRGDFGVSMWTGRPVLYEISIRFQLSL
                     QLAIMAAFLAVLLAIPLGTMAALRQDTWVDYVIRVFSIAGLATPSFWLGIIVILGLLI
                     LFGWLPPMEFSPFWENPASNFAQLIWPTLAIGYRYSAMIMRMTRSSVLEVLREDYIRT
                     ARAKGLREKLILVRHALKNAMLPVISVIGLELAFLVGGMVVTEQVFNLNGLGRLLVQS
                     IEQRDYTMTQSLVLLTAVFFIVMNFVTDLLFAWLDPRVRYK"
     gene            2449..3306
                     /locus_tag="VEL68_12485"
     CDS             2449..3306
                     /locus_tag="VEL68_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36830.1"
                     /translation="MVFSGKMQRVKRFCRQRPLGAVGGLIFLIMVFTALLADWISPYQ
                     PMVNDYKAMLSPPSMNHFMGTDAHGRDVLSRIIYGSRTALLVGFSSSFCGTILGAVIG
                     VLCAYFGGKTDVIVQRFMEILLSFPLIVMALAMMAVLGTGLLNVIVAITIPMIPRSAQ
                     VIRSSALSIRSMPFVDAARACGFGHIRIMFRHILPNVAAPFLILVTAYLGGAILTEAS
                     LSFLGVGVAEPTPAWGLMLKGAAVTFAETAPWMAIFPGLAISAAVFAFNIFGDSLRDE
                     LDPKLRTSS"
     gene            3388..4392
                     /locus_tag="VEL68_12490"
     CDS             3388..4392
                     /locus_tag="VEL68_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36831.1"
                     /translation="MLLEVKNLRTQFFTPEGVVRAVDGVSFDIQEGETVALVGESGCG
                     KSVSALSIMGLIPNPPGRIVEGEIQFRGRDLRLLDREEMRKIRGKEIAMIFQEPMTSL
                     NPVLTIGRQLTEALEMHLQMKTQEAGNRAIEFLKLVGIPDPQRRLSQYPHQFSGGMRQ
                     RVMISMALCCEPRLILADEPTTALDVTIQAQILELLKDTCRRLHVAIIIITHNLGVVA
                     RYADRVNVMYAGKIIETGSSLDIYLSPRHPYTLGLLRSVPRLDQPRKAKLDPIEGQPP
                     DLADLPPGCSFRARCKFAREKCAQEAPFLFEVGENHWSSCWMWERVGGGSARSPGSGE
                     "
     gene            4416..>5104
                     /locus_tag="VEL68_12495"
     CDS             4416..>5104
                     /locus_tag="VEL68_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ36832.1"
                     /translation="MNSLRQPIAAKSSNGTILEVRNLKKHFSLTSSMILQRVVGWIKA
                     VDGVSFSIRRGETLGLVGESGCGKTTTGRCILQLDRPTSGEVLFEGKELTQVPDRELR
                     SIRPRIQVIFQDPFSSLNPRMTIGQIVGEGPAVFKLVKSGRELRERVAGLINTVGLNP
                     NMATRYPHELSGGQRQRVGIARALSMEARFLICDEPVSALDVSIQAQIINLLEDLQEK
                     LNLTYLFIAHDL"
BASE COUNT         1021 a         1453 c         1556 g         1074 t
ORIGIN      
        1 ccgggtcctg gatgagatgg cttatgggtt cccgccggtg ggatggacca agcggattac
       61 catacattcg gccaaggtaa agggctgggt cgcactcccc caccactttc tcaatgtaga
      121 cctggtggat gtgtggttgg aaaaggattg aggcaagaac cgcttttgct tgcgtagggg
      181 cgattcatga atcgccctta caaacatgct taattcccag acccttgcgg taataaaaag
      241 aggttttatg cttccttcct gtaaagaccc ttttaaggtc gtacgccgat ccaaactgtt
      301 cattcccgtg aaccgggaga aattcgtggc caaggcctgg acccgggggg ccgactgcct
      361 catcctggac ctcgaagatt cgattccccc ggcggagaaa tcctcggccc gccggatggt
      421 gaaggatgtg atccctctgg tgaaaaaggg cggagcagag atcgaggtgc gcatcaaccg
      481 ggattatgag gaagaggacc tggcggccgt cgtggggccg gaggttgcca gcctgatgat
      541 ccccaagtgc gagtcagcgg aggaactccg gcggcttgac cggagggtgg cccagctcga
      601 gaaggaacgt ggggtccctg agggaagaac cgacttctcc ctcctcatcg aaacggcccg
      661 gggcgtggtc cggatggagg agatcgtggc agccagcccc cggatcgtgc aggccagcat
      721 cggcggcgtg gacctgaccc aggatatggg ttttacccgc gtacgggacc tgctggcgga
      781 gcaattctcc tatccggccg gcaaactcct ctacgctgcg agggccgcgg gggtccaggc
      841 ggccgggctt tggccgcaga acaatgtgga cttcaccgac accaccctgt cctacgaagc
      901 catgctccat gcctgccgaa ggtccttcca gatgggatac ttgggaagct ccaccatcca
      961 tccggcctgg gtccggccac tcaacgaggg cttcaaaccc tcggcctccg agttggattg
     1021 ggcccgtcgg gtcaaggttg ctctggaaga ggcctacagt caggggctgg gatcggtggc
     1081 cgttgacggc cgcatggttg acgtggctaa tttgaaacag gtccagaaga ttctggagcg
     1141 ggcggaggcc atcgaccggc gggaagccga gaaggaaaca gcgatggcgg ccaccggcgg
     1201 gagagcgtaa aacggcggag ttaaaagtta ctatggagtc cataattgaa aagacataat
     1261 cttccgaaaa tctcctctaa ccctggtgct catcacctgg actgtctccg ttcaccctga
     1321 gcttgtcgaa gggtgggtcg ccacgccgtt catgcttcga caaactcagc atgaacggct
     1381 tagtcttcag cgttcataca attatggact gattagtaaa aaagtatgcg gcaatacatt
     1441 ttaaagcgga tcctgcttat ggttcccacc ctcctggggg tggcggtcct tatctttctt
     1501 ttgatgcggg tcgtgcccgg cgatttggtg gagctgaagc atgcgggaga aggcagcgcc
     1561 ctttcggcgg agaccctgca gaaggaacgg gagcttcttg ggctggacaa accgctatgg
     1621 tatcagttcg gagtgtgggt ctgggacatc ctccgggggg actttggcgt ttccatgtgg
     1681 accggccgcc cggtccttta tgaaatttct atccggttcc agttgagcct gcaactggcc
     1741 atcatggctg ctttcctggc ggtcctatta gcgattcccc tgggtacgat ggccgcccta
     1801 aggcaggaca cctgggtgga ttacgtcatc cgagtctttt ccatcgccgg cctggccacc
     1861 ccttcgttct ggttgggcat catcgtcatc ctgggactcc tgatcctctt cggttggctt
     1921 ccccccatgg aattctctcc cttctgggaa aatcccgctt ccaactttgc ccaattgatc
     1981 tggcccaccc tagccatcgg gtaccgctac tcggccatga tcatgcgcat gacccggtct
     2041 tcggtcctgg aggtgttgcg ggaggattac atccgtacgg cccgggccaa ggggttgcgg
     2101 gagaagctga ttctggtacg ccatgccctg aagaacgcca tgttgccggt gatcagcgtg
     2161 atcggcctgg agctggcctt tctcgtcggg ggcatggtgg tgaccgagca ggtgttcaat
     2221 ctcaacggcc tcggccgcct gctcgtccag agtatcgaac agcgcgatta taccatgacg
     2281 cagagtctgg tcctcctgac agcggtcttc ttcatcgtca tgaactttgt gaccgacctc
     2341 ctcttcgcct ggcttgaccc gagggtccgt tataaatagg gaaggagatt tccgaatccc
     2401 tgccgagctt tttgccgttg gcaaggaaaa gagaaaggaa gaaagggaat ggttttctca
     2461 ggcaagatgc agcgggtcaa acgcttttgc cgccagcggc cgctgggggc cgtcggggga
     2521 ttgatcttcc tgatcatggt cttcacggct ctcctggctg attggatttc cccttatcaa
     2581 cccatggtaa acgactacaa ggccatgctt agcccaccga gcatgaacca cttcatgggc
     2641 acggatgccc acggaaggga cgtgctctcc cgaatcatct atggatcccg gaccgcgcta
     2701 ctggtgggtt tttcatcctc cttctgcggg accatcctgg gagcagtcat cggagtcctg
     2761 tgcgcatatt tcgggggaaa aaccgatgtg atcgttcagc gattcatgga gattctcctc
     2821 tccttccccc tcatcgtgat ggctctggcc atgatggccg ttctgggtac cggcctcctc
     2881 aacgtcatcg tggccatcac cattcccatg atcccgcgca gcgcccaagt gattcgatcg
     2941 tctgccctgt ccatccggag tatgcccttt gtggacgccg cccgggcctg cggattcggc
     3001 catatccgga tcatgttccg gcatattctg cccaatgtgg ccgctccctt cctgatcctg
     3061 gttaccgctt atctgggggg agcgatcctg accgaggcat cgctcagttt cctgggagtc
     3121 ggagtggccg agcccacgcc cgcttggggt ctcatgctga aaggggcggc agtgacgttt
     3181 gccgaaactg ccccctggat ggcgatcttt ccgggattgg ccatcagcgc agccgtcttc
     3241 gcctttaaca tcttcggaga ctccctccgc gacgaattgg accccaagct ccggacttcc
     3301 tcctgaggaa aagcgaagag agattcgtgg agaagagagc cgggtgagga aagggctgat
     3361 gaaggttggg gaaatgaagg aacgagcatg ctgctggaag tgaaaaatct cagaacccag
     3421 ttcttcaccc cggagggggt ggtccgggcc gtggacggag tctcttttga catccaggag
     3481 ggagaaaccg ttgccctggt tggggagagc ggctgcggca agtcggttag cgccctttcc
     3541 atcatgggtc tcattcccaa ccctcccggg cggatcgtag agggagaaat ccagtttcgc
     3601 ggccgggacc tacgtctgct tgaccgggag gaaatgagaa agatcagggg caaagagatc
     3661 gccatgatct tccaggagcc catgaccagc ctgaacccgg ttctgaccat cggccgccag
     3721 ctgaccgaag cgttggagat gcacctccag atgaagaccc aagaggcggg gaaccgggcc
     3781 atagagttct tgaaattggt gggaatcccc gacccgcagc ggaggctctc ccagtacccc
     3841 caccagttca gcgggggcat gcgccagcgg gtcatgatct ccatggccct gtgctgcgaa
     3901 ccccggttga ttctggctga cgagccgacc acggccctgg atgtgaccat tcaagcccag
     3961 atcctggagc tgctgaaaga cacctgtcgg cgcctccacg tggccatcat catcatcacc
     4021 cacaatctgg gagtcgtggc ccgctacgcc gaccgggtga atgtcatgta tgcgggcaaa
     4081 atcatcgaaa ccgggagttc cctggatatc tacctgtccc cccgacatcc ttacaccttg
     4141 gggctgcttc gttccgtgcc caggctggat cagccgcgga aggccaaact ggaccccatc
     4201 gagggtcagc ccccagactt ggcggacctg cccccgggct gcagcttccg ggcacggtgt
     4261 aaattcgcca gggagaaatg cgcccaggaa gcaccgtttc tgttcgaagt gggggaaaac
     4321 cactggagtt cctgctggat gtgggaacgg gtgggaggcg gttctgcccg atccccggga
     4381 tcgggggagt agattaccgc aagaaaggtc agcgggtgaa tagtttgagg caaccgatcg
     4441 ctgccaaaag ttcaaacggg acgatcctgg aggttcgcaa cctcaagaag catttttccc
     4501 tgacttcttc catgatcctc cagagggtgg tggggtggat caaggctgtc gacggggtaa
     4561 gcttctccat ccgaagggga gaaacgctgg gactagttgg agaatccggg tgcgggaaga
     4621 ccaccacggg gcgctgtatc ctgcagctcg accggcccac ttcgggcgag gtcctcttcg
     4681 aagggaagga actgacccag gttcccgacc gggagctgcg gtccattcgc ccgaggatcc
     4741 aggtcatctt tcaggaccct ttcagttccc tgaatccccg gatgaccatc ggccagatcg
     4801 tgggggaggg acccgccgtt ttcaaactgg tgaagagcgg aagggaattg agagaacgag
     4861 tggccggatt gatcaacacg gtgggactga acccgaacat ggcgacccgc tatccccatg
     4921 agctcagcgg ggggcagcgc cagagggtgg ggattgcccg ggcgctttcc atggaggccc
     4981 ggttcctcat ctgcgacgag ccggtatcgg ccctggatgt gtccatccag gcccagatca
     5041 tcaacctgct cgaggacctg caggaaaagc tcaacctgac gtatctcttt atcgctcatg
     5101 acct
//
LOCUS       DASPRR010000336         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1204329, whole genome shotgun sequence.
ACCESSION   DASPRR010000336 DASPRR010000000
VERSION     DASPRR010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204329"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VEL68_12500"
     CDS             <1..272
                     /locus_tag="VEL68_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HXZ36833.1"
                     /translation="IRDLSHAHPKIRDLISHLDIFRWGHAMVQPRVGFIWGEARKRAA
                     RPHGNIFFAHSDLSGFSIFEEAQYRGVLAAERILAKQKVPFSSSL"
     gene            586..1161
                     /locus_tag="VEL68_12505"
     CDS             586..1161
                     /locus_tag="VEL68_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36834.1"
                     /translation="MEKKEKPIPKLKLIQGGRRSEVSFGSVRITAAPETHPPFPVEAV
                     VFEEDTYLVLSADWKKIESEDHPVVILTEAFGMEPEEPGRVLVYEGSPLRFLAVVHDL
                     DRAPSWREEWVRKALENAFQEVEGRGIRSIALPFLGTKHGSLEKRRFVGLLVDFLKKN
                     PFSHPLRIWLAMPGDTGGEIIEWLEADRKKG"
     gene            complement(1124..1972)
                     /locus_tag="VEL68_12510"
     CDS             complement(1124..1972)
                     /locus_tag="VEL68_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ36835.1"
                     /translation="MGKKILLVDDDKDLVYGMKVWLRANGYHVLFAPDGPTAITMAQE
                     EKPDLILLDIGLPGGDGYMTMARLRTLMPQPHIPILVITARDAITHEKKALEAGAEGF
                     FQKPFDNRHLLAAIQKALGEKPALKNPKQESPKKTPIEAKGKKILIIDDDQELLQALR
                     VRLTSIGFDVKLAPDAVLAIQLALKIKPDLILLDIGLPGGDGYLTMTRLQSHLHLAQV
                     PIIIMTAADASIHRDRVLEAGADAFLQKPIDNQQLLAAIYGALGETKTRTPIFSLSSD
                     PLQATR"
     gene            complement(1972..3543)
                     /locus_tag="VEL68_12515"
     CDS             complement(1972..3543)
                     /locus_tag="VEL68_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXZ36836.1"
                     /translation="MKPSIKVLLVEDNPYDAELLREMLLGVPSVQFDWVQVALLGDAL
                     KRLREDTFDAILLDLSLPDSHGQETLAKAYAQAHDTPIVVLTGHADESLAIKTVHQGA
                     QDYLVKGRVDGPTLVRAVRYAIERHRLLTELDRGRQQQLKIKDQFLSHVSHELRSPLA
                     VIHQFVTILLDRLGGDLSPEQNEYLEIILRNVNQLRKMIEDLLEVTRAETGKLSLQPQ
                     LTPLDELITETLGALRIAAAGKRITLSESVPDDLPLAYADPNRFRQVLMNLIDNAIKF
                     TPQGGSITVHAELFPQDPHFIQVAVADTGCGISEEDCHKIFDSMYQVKDSAPLSRKGL
                     GLGLFISKELVSRHGGQIWVESEKDKGSTFFFTLPIFSLPQQLAPILHSRNLKLGSVA
                     LITVEVFSPSKRDLTKTDDGILWEVGTILKHCTLIDKDFLLPRMAPTKWKEYFFIIAF
                     ANKKGSEILQQRIQEQVGGYQKLQDAGLEAAITSQVFEIPPMRDGLPENSFVKDLTWS
                     IKDRIRKAFQEEEAR"
     gene            complement(3554..4000)
                     /locus_tag="VEL68_12520"
     CDS             complement(3554..4000)
                     /locus_tag="VEL68_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ36837.1"
                     /translation="MNYTKGGRPIQILMVEDNPDDIELTVEALKDSRVRNHLIVVKDG
                     EEAVSYLQGKGQYQHAVRPDLILLDLNMPKKNGRDVLREIKNDPKLKRIPVVILTTSQ
                     AEEDIIHTYDLHANCYITKPLDFNQFLKVVKSIEDFWLTVVKLPPR"
BASE COUNT          866 a         1035 c         1162 g          962 t
ORIGIN      
        1 tcatccgaga cctctcccat gcccatccca aaatccggga cctgatctct cacctggaca
       61 tctttcggtg gggccatgca atggttcagc caagggtggg tttcatctgg ggggaggctc
      121 gcaaacgggc ggctcgcccc cacggaaata tattttttgc tcattctgat ctaagcggct
      181 tttccatttt tgaggaagct caatatcgag gagtattggc cgccgaaaga atcctggcca
      241 aacagaaggt tcccttttct tcctccctgt gaaggagcaa gaggcataga gcataacaaa
      301 aaaaattgca gaagagggaa cagtttggtg gtttgaaacg ggcttcccag aaacccctcc
      361 ggacctccct tttccaaagg gaggagaggt tacttacgga agttcgatga atcgtgaggc
      421 cttttccccc tttgaaaaag ggggatggag ggggatttga atcctgcggt actgcagtcg
      481 atgttctgaa gaaaagaatt tagaaagcgc ctacgctctg tctttgtaaa gaagcaggtg
      541 acggtttagg ctttcgagat ttgctcgaga aggtctggga agaaaatgga gaagaaggaa
      601 aagccgatac caaagctcaa acttattcag ggcgggcgtc gatcggaggt ctcttttggt
      661 tcagtccgga tcactgccgc gcccgaaaca cacccccctt tcccggtgga ggcggtcgtc
      721 ttcgaagagg acacctatct tgtcctgagc gccgactgga aaaaaatcga atcggaggat
      781 caccccgttg tgatcctgac cgaagccttt ggaatggaac cggaggagcc tggaagggtt
      841 ttggtttacg agggatcgcc cctccgtttc ctggcggtcg tccacgacct cgatcgagcc
      901 ccctcgtgga gagaggaatg ggtccgaaag gctctggaga atgccttcca ggaggtcgag
      961 ggccgaggaa ttcggtcgat agcccttccc tttttaggaa ccaagcatgg ttcgctggag
     1021 aagaggcgct tcgtcggcct tctcgttgat ttcctgaaaa aaaatccctt ttctcatccc
     1081 ctgcggattt ggctggccat gcccggggac acggggggtg aaatcatcga gtggcttgaa
     1141 gcggatcgga agaaaggctg aatatcggag tccttgtctt cgtttctccc agggctccgt
     1201 agatagcggc caggagctgc tggttgtcga tgggtttctg gaggaaagca tcggccccgg
     1261 cctccaggac ccggtcccgg tgaatcgatg catcggctgc ggtcatgatg ataatcggga
     1321 cctgagccag gtggaggtgg gactgcagcc gggtcatggt caggtagcca tcccccccgg
     1381 gcagaccgat gtccagaaga atcaggtcgg gcttgatctt gagagccagc tgtattgcga
     1441 ggactgcatc aggggctaat ttaacgtcaa atccgatgga tgtcagccgg acccgcaacg
     1501 cttgaaggag ttcttgatcg tcgtcgatga tcagtatttt tttccccttg gcctcgatgg
     1561 gtgtcttctt gggggattcc tgcttcgggt ttttgagggc aggcttttca cccaaggcct
     1621 tctggattgc ggccagaaga tggcggttgt caaaaggttt ctggaagaaa ccctccgccc
     1681 ctgcctcaag agcctttttc tcgtgggtga ttgcatcccg ggcggttatg accaggatgg
     1741 gaatatgagg ctggggcatc aaggtccgca gccgggccat ggtcatatag ccatctccac
     1801 cgggcaggcc gatgtccaga aggatcaggt cgggcttttc ctcttgggcc atggtgattg
     1861 ccgtagggcc gtccggggca aagaggacat gataaccgtt ggcccgcaac cagaccttca
     1921 tcccgtagac caaatctttg tcatcgtcca ccagaaggat cttcttcccc attaccttgc
     1981 ctcctcttct tgaaaggcct tgcgaatccg atctttgata ctccaggtca ggtccttcac
     2041 gaacgaattt tccggaaggc cgtctctcat aggaggaatc tcaaagacct gcgaagtgat
     2101 ggccgcctcc aaccctgcgt cctgaagttt ctggtaaccg ccgacctgct cctgaatccg
     2161 ctgctgaagg atttcggaac ctttcttgtt ggcaaaggcg ataataaaga aatattcttt
     2221 ccatttggtt ggggccattc ggggcagcag gaaatccttg tcgatcaggg tgcaatgctt
     2281 caggatggtt cccacttccc agaggatccc gtcatccgtt ttggtcaggt cccgcttgga
     2341 gggggagaag acctccacgg tgatgagggc gacggaccct agtttcaggt tcctggagtg
     2401 gaggatgggg gccaattgct gcggcaggga aaagatgggc aaagtgaaga agaaggtgct
     2461 tcccttgtct ttttcgcttt ccacccagat ctgccccccg tggcgggaga ccagctcttt
     2521 gctaataaag aggcccagac ctaaaccttt gcggctcagg ggggccgagt ccttgacctg
     2581 atacatggaa tcgaaaatct tatggcagtc ctcctcactg atcccgcacc ccgtatcggc
     2641 tacggccacc tggataaagt gggggtcttg cgggaatagc tccgcgtgaa ccgtaatact
     2701 cccgccctgc ggagtaaatt ttatggcatt gtcgatgagg ttcatgagga cctggcggaa
     2761 gcggttggga tcggcgtagg ccaaaggcag gtcatcgggg accgattccg acaaggttat
     2821 tctttttccc gcggcggcaa tccgcaaggc gccgagggtc tcggtaatca attcatcgag
     2881 aggagtcaac tggggctgaa ggctgagctt ccccgtttcc gcccgggtca cctccagcaa
     2941 atcttcgatc atcttgcgaa gctggttgac gttcctcagg atgatctcca aatactcgtt
     3001 ctgctccggg gagaggtccc ctcccagacg atccaaaagg atggtcacaa attggtgaat
     3061 cacggccagg ggggatcgaa gctcatggga cacgtgggac aaaaattggt ctttaatctt
     3121 cagctgctgc tggcgtccgc ggtccaattc agtgagcagg cggtgccgtt cgatggcgta
     3181 ccgcacggcc cgcaccaggg taggcccatc gacccggcct ttcaccagat aatcctgcgc
     3241 accctgatgg acggttttga tggcgaggct ctcgtcggca tggccggtta aaactacgat
     3301 gggggtgtcg tgggcctgag cataggcctt ggccagggtc tcctgtccat ggctgtcggg
     3361 aagggaaagg tccaggagaa tggcatcaaa ggtatcttcc cggaggcgtt tcaaggcgtc
     3421 ccccagaaga gccacttgga cccagtcaaa ttgcacggaa ggtactccca aaagcatctc
     3481 ccggagcagt tccgcgtcgt agggattgtc ttctaccaaa aggactttaa tggacggttt
     3541 catttttctt tttctaccgg ggcggcaatt tcacaacggt cagccagaaa tcttctatcg
     3601 attttaccac ttttaaaaat tgattgaagt ccaaaggttt ggttatatag cagttggcgt
     3661 gtaggtcata ggtatggata atatcctcct ctgcctggga ggtggtgaga ataacgacag
     3721 ggatcctctt cagcttgggg tcgtttttga tctcccggag cacatcccgg ccgttctttt
     3781 tgggcatatt caggtcgagt agaatcaggt ccggacgaac ggcgtgttgg tattgtccct
     3841 tgccctgcaa gtaagagaca gcctcttccc cgtccttgac cacgatcagg tgatttctca
     3901 cccggctgtc cttgagcgcc tcaaccgtca gttcgatatc atcggggttg tcctcgacca
     3961 tcaggatttg aatgggtcgt ccgcccttcg tataattcat gaacaattgc ctcctgggtc
     4021 gggga
//
LOCUS       DASPRR010000337        48574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_82869, whole genome shotgun sequence.
ACCESSION   DASPRR010000337 DASPRR010000000
VERSION     DASPRR010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 48574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48574)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48574
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82869"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VEL68_12525"
     CDS             complement(<1..1308)
                     /locus_tag="VEL68_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ36838.1"
                     /translation="MDMSTSKMRRPSLDYPEESVSHFLKKTVGRLPNKVAVIDVHGER
                     VLTFLEMDRESDALAQAFLDWGIRKGDRVSFFLPNGWEYFVGFYAAMKMGAIVSPMNP
                     TLREREVKNQVNDAESRVILIQKDLFPVVEKILPQMPSLEKVILTRGKEPSGRRVFSL
                     QNLLQKYSAPLSLSLPPIQTKADLAALPYSSGTAGLSKGVMITHFNLVSNTIQSMNAV
                     EARESEIFISFLPFHHIYGFTYFLCGSIYLGATQVVMSRFHAEECLGLMEKYRVTMIF
                     GVQPALLAFLNVPDLERFDLSSLRYIWIGAAPLVPAIARTIQEKFRVPIARHYGLSEA
                     SPTTHSNPPDRPKEGSVGIAVSDMEDKIMDWDKGETEMKLGEPGELVVRGPNLFQGYW
                     KHPEDTRLALRDGWLYTGDIARMDEEGYVFILDRKKEMIKYRGY"
     gene            complement(1350..2537)
                     /locus_tag="VEL68_12530"
     CDS             complement(1350..2537)
                     /locus_tag="VEL68_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36839.1"
                     /translation="MKFHFFLGIIGFSFLLLPMFFPLTTHAELLKIGLLIPWQDSGTG
                     ADSSYARGAEIAVAEVNAVEGKQGIQLLLLARRGTFLDRREQSVLRDFFFEQRPSFLI
                     GALHREAIIPVSRLAQEHQIPILAFPIEFMEAVSTGDEPSGLFWITPTLEAFQRAAVR
                     TAAQFSKKRFYLLARNSALGRNWVGYFWEAMKKQNPEAQRLGETFLPPRVDDYRPYLR
                     TLMALNTEVCLSHLGVKDWLEFYQLANKEGYFKKIIHLELESGNLDSLVGMRKKVPEG
                     VWGISAFPFWALDWKETKVFVSKYRNNTNSYPSLAALSGYISIHALIKAIQKAGSLDS
                     EKAFRALEDLTFPTPVGPMTIRKSDHRTMWPIWGGSIQSTSDYPFPIIGNLKSLGPDS
                     FSP"
     gene            2871..4199
                     /gene="ffh"
                     /locus_tag="VEL68_12535"
     CDS             2871..4199
                     /gene="ffh"
                     /locus_tag="VEL68_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXZ36840.1"
                     /translation="MFDSLSDKLSTVFKKLRGHGKLTEQNISEALKEVRLALLEADVN
                     FKVVKDFIERTQTRAVGQEVLRSLTPAQQVVKIVHEELISLMGGSSSGLNLSYKPPIP
                     IMLVGLQGSGKTTTAAKLGKFLKDKGRKVHLVPADVRRPAAVEQLKKLGEQLAIPVYP
                     TDPQESPVKICRKALQWADEESGDVLLIDTAGRLHIDEALMKELEEIKKEVTPREILL
                     VADAMTGQDAVTVAKKFNDSLDLHGVILTKLDGDARGGAALSIKAVTGRPIKFIGIGE
                     KLEALELFHPDRMASRILGMGDVLSLIEKAQEAIDEKKAQELQKKLLKDSFTLEDFRD
                     QLQQIKKMGSIEEILSMIPGMGRLKVPKDLQGSEKELIRVEAIINSMTRQERHHPEIL
                     NGSRRLRIAKGSGTTVQDVNQLLKQYLETKKMLRTFRKGGMKGLPRGLFP"
     gene            4371..4679
                     /gene="rpsP"
                     /locus_tag="VEL68_12540"
     CDS             4371..4679
                     /gene="rpsP"
                     /locus_tag="VEL68_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXZ36841.1"
                     /translation="MAVTIRLTRMGSKKKPFYRLVAADSRFPRDGRFIEVLGTYDPKT
                     NPPAIKLNREKVEAWLNQGASVSATVKSLLKKGGVSLRPTQKSTPANQEGGPRAESPA
                     "
     gene            4791..5021
                     /locus_tag="VEL68_12545"
     CDS             4791..5021
                     /locus_tag="VEL68_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXZ36842.1"
                     /translation="MKELIAYIAKALVDAPEAVEVSEVEGEQTSVIELKVAKGDLGKV
                     IGKQGRTARAMRTILNAASTKIKKRAVLEIIE"
     gene            5029..5553
                     /gene="rimM"
                     /locus_tag="VEL68_12550"
     CDS             5029..5553
                     /gene="rimM"
                     /locus_tag="VEL68_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXZ36843.1"
                     /translation="MEDPLLWVGRLVKTQGIKGEVRVSSPGREALAQSRGKVVYLEDP
                     KGNRKAFTVAASRPLRELTVLAFDEIKSIEEAQNLIGCSVYVNKANLSPLPPDEFYWY
                     QLRGLRVETGEGKFLGILEEVFSTGSNDVFVVRKEGKEILLPATDEVVAQVNLQEKVM
                     IIHPLEGLLPEDGL"
     gene            5543..6283
                     /gene="trmD"
                     /locus_tag="VEL68_12555"
     CDS             5543..6283
                     /gene="trmD"
                     /locus_tag="VEL68_12555"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXZ36844.1"
                     /translation="MVFDILTIFPEIFRSPLSESLIKKALDKGVLQVRIWNLRDFTED
                     KHRTTDDYPYGGGAGMVMKADPIFRAVEEIKANNPDARTLLLTPQGERFHQGLAKGLS
                     EQKHWILICGRYEGLDERVRLGVVDREISIGDYVLNGGEIPALVFLEVISRYVPGFLG
                     SEQSVEEETFSDGLLEYPQYTRPPVIRGMEVPEILLSGNHAEIKRWRRRESLKRTYLR
                     RPDLLERADLSEEDLEYLAELKSQKARE"
     gene            6384..6731
                     /gene="rplS"
                     /locus_tag="VEL68_12560"
     CDS             6384..6731
                     /gene="rplS"
                     /locus_tag="VEL68_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXZ36845.1"
                     /translation="MRAIEQLEKEQMRMDIPDFRAGDTVKVHVKIKEGEKERIQVFEG
                     IVIRKRQGASRASFTVRKVSYGVGVERIFPTHSPAIDHIDVVQRGKVRRAKLYYLRAL
                     RGKAARIREQREN"
     gene            6854..7441
                     /locus_tag="VEL68_12565"
     CDS             6854..7441
                     /locus_tag="VEL68_12565"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXZ36846.1"
                     /translation="MKARSEGFQVIAGLDEAGRGPLAGPVVAAAVVLPPTRNLKGVAD
                     SKTLTADRREKAFFLIQKKALGMGIGIVEAEEIDRLNILRASLKAMELALQGLSLSPD
                     CLLIDGLHTLNLPLKQQAITKGDGRCLSIAAASIVAKVTRDRLMVEYHEKYPHYNFAR
                     HKGYGTREHLQAIREHGCCPIHRQSFRTIYQLSLL"
     gene            7438..7809
                     /locus_tag="VEL68_12570"
     CDS             7438..7809
                     /locus_tag="VEL68_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXZ36847.1"
                     /translation="MTQDRLSLGKKGEELAIAQLEALKYKILERNFKCPLGEIDIIAR
                     DKSTLVFIEVKTRATKDFGGPAAAVHERKQRQLSKVALVYLNQKKLFNIPARFDVVAV
                     ELIPPYPRIEVIRNAFDLCYE"
     gene            complement(7913..9082)
                     /locus_tag="VEL68_12575"
     CDS             complement(7913..9082)
                     /locus_tag="VEL68_12575"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ36848.1"
                     /translation="MLDFSEVPIIDNHSHPFDLEKVTLDADSLAKVFFHGMGDIPKEG
                     VKKAKLWGTTDELRYHLRHMGVIQTMVCQLSKLLGCPDELEAVAAERSRRTSKNFPAY
                     VRLLYEDAGIVGSVVDAGLSMKDPLLDLIPGKVMRLFQMDPLIDNLLERTDSYKEFLR
                     EYQEGLSGAAKQEGFCGVKCHLAEQVGFGVEPVSDAEAEAIFPAAKAKDSDAYKELYV
                     AAFTHTLLQCQDLRIPVHLHSGCTGGLWDGPISNADPFLLVPLIRQPRFLQTRIVFLH
                     AAYPWLQHAAELAHSLPHVWVDMSWTTPWVSLRLVDCYKEVIGIAPLSKIIVGSGGHD
                     TPEIPWLAAKTAKLALAEALGDAVRFGLLTFKQAEKAGRMILHENAARMYGLDKY"
     gene            9202..10068
                     /locus_tag="VEL68_12580"
     CDS             9202..10068
                     /locus_tag="VEL68_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36849.1"
                     /translation="MDQDILRDQEGFRQQVIDALSKEPVDYVEQFAFIKREKETQENF
                     SGGGVLLPLFFGEGGNWGEAGPGEYVFLLNKRSKQVLQPGDLCAPGGGIHPFWDSFTQ
                     KILQFKLLPGVGGPGLKLAKKRGKEVYEKILFLLGNALRESWEELRLSPFNVEFLGPL
                     PTYSLSSRRWIIFPLVGRVKRSWQPKLSWEVEKIVPIPLSAFFKPGNYAVYSLEVPEK
                     LVAHGIPSPWEFPCLVHREKGEEEILWGATFKVIQTFFQIVLGFSLPYPDGGQVIRRP
                     LASNYLTGREEL"
     gene            10388..11269
                     /locus_tag="VEL68_12585"
     CDS             10388..11269
                     /locus_tag="VEL68_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36850.1"
                     /translation="MDAIGSTIVIQVLHGLAYGMLLFLVASGLTLVFGMMSVLNLAHA
                     SFYMLGAYFSYQMLKWVGNFWIGLILCPILVGLLGILMERFLLRRVHASGHVQEFVLT
                     AGIAFVVMEVVKWVWGTEPLPVPIPAALSGSLNIVGNVYPAYRLFILFFSIVVLAFMA
                     YILMKTRLGITVRAAVADRDMVSALGTNTPFVFMLVMGIGTWLAGLAGVIAGPFISTY
                     PGMAGEILVDCFVVTVTGGLGSLVGAVVASFLIGQLSSFGILLIPRFAIVFAFLLMAI
                     VLIIKPSGLFGEKGRVS"
     gene            11266..12192
                     /locus_tag="VEL68_12590"
     CDS             11266..12192
                     /locus_tag="VEL68_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36851.1"
                     /translation="MKTKKGLLLAVAFAVFLILFPFFAPKFQLRMFTEIIILTLFAMT
                     WKMMLNEGGMFSFGHAVYFGLGAYASVLGWLHIPGLSFLSGILLGALVSALVAFLLGA
                     FLVRMSGTYFALLTLAFNQLIWAIVWKWRDVTGGDDGLGKFPKPDLFGLAMKNPVSFY
                     YVALVIIGIGLYICWYFTKTPMGNVALSIKSNEERAKFVGFNVQATKLIFLTLLGFLA
                     GISGALYAQFQEFIATSVIDGMMSTNVLFMAYIGGTGFFWGPIIGSGIFVYLSEYLSS
                     FTDRWELILGLIFIAVVLFAPQGIMGVVRKKK"
     gene            12205..12981
                     /locus_tag="VEL68_12595"
     CDS             12205..12981
                     /locus_tag="VEL68_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36852.1"
                     /translation="MAETAGKLGNPILQLQGIYKDFEGLEVLFGINLAIQQGERHAII
                     GPNGAGKSTIFNIITGKYLPSKGNIFFKGQDVTGTSPYKLNRHGLARSFQITNIFRTM
                     TVFQNVRNAILSRNKIRYNLFSRLDRMMNIQEQTDEVLEQIGLLDRKDVVAGLLSYGQ
                     QRALEIGLTIATEPELILLDEPTAGMSSEETREAVKLIGRVTQGRTLIIVEHDMEVVF
                     SLADRITVIYYGEILASGPPDEIRQNQKVKDAYLGEEKEE"
     gene            12985..13692
                     /locus_tag="VEL68_12600"
     CDS             12985..13692
                     /locus_tag="VEL68_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008229813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36853.1"
                     /translation="MLLEIQNLNTFYEQSHILQGISLNIDTGEIVCLLGRNGVGKSTT
                     LKSIIGLVSPQSGEVLFKGRNVAGMPPFDIAKMGVGYVPEDRRIFPTLSVRENLIMGI
                     KPGQKSNGDGWSVEKVYKYFPGLQARDKQKGGFLSGGEQQMLTIARTLMGNPEVLLVD
                     EPTEGLAPLIVKTVEQVIQDIHQHNIPILLVEQNMRVALRLAGRIYVMSKGKIVFHGT
                     SQELKEAHEVREKYLEV"
     gene            13780..14985
                     /locus_tag="VEL68_12605"
     CDS             13780..14985
                     /locus_tag="VEL68_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36854.1"
                     /translation="MAKRVLLFVFLIALFTSPGWAQDTVTMIQLDPLSGPFKDVGDLS
                     IAGVQFLVDEVNAAGGLLGKKIKYMPEDSQLKPDVAVRKATKAIMEDNAKIILQLSST
                     AVARALMDVAEKNKVIFVTLGAESDFLTGKDFNPYFFRTCFTTANRSKSYAEFFKTKP
                     WRKVYLINMDYAFGHAVADDFKVTMKKEIPDLKIVGEDYHPLATKDFGPYISKILASG
                     AEIIFTGNWGTDLEVLIRQGTQMGVKTRYATYFLDDRVRLSNIGEFAVGSFVNSTYLP
                     TIDTPQNKGFLERWHKKNKDTKDPWPTAQIGYGYNGVSFLFEAIKKAKSFDADAVIKA
                     WEGMEYGSLVGKQIMRACDHQILMPGPVGEIQAKSSLFPFPFPGAPLMVPMDKVAVPF
                     KETGNPRCK"
     gene            15080..15976
                     /locus_tag="VEL68_12610"
     CDS             15080..15976
                     /locus_tag="VEL68_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXZ36855.1"
                     /translation="MAIKKISVIGLGTLGTQIAIQAAYYGYEVRGYDQDPQTFQKTIQ
                     KIKGMMKFLGKNPTMGAEEWEKGAGKVKLAKDLAEAVKDGELVIEVVPEVLELKRKVW
                     GQIDSLAPKGALLATNSSSIPVSRIESATQRPEKCMNIHFYQPAVGWNIVDMMGGTQT
                     LPETLETAKQFIRSIQCIPLTVKKEILGFCFNSVWRAIKRQTLYLWGEGFVDFRDIDR
                     AWMVMFTPTHGPFFLMDLVGLDVVYDIEISYYNESKDPKDYPPKALKDMIERKELGVK
                     TGKGFYTYPNPEFGRPEFLKGK"
     gene            complement(16106..17167)
                     /gene="dnaJ"
                     /locus_tag="VEL68_12615"
     CDS             complement(16106..17167)
                     /gene="dnaJ"
                     /locus_tag="VEL68_12615"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXZ36856.1"
                     /translation="MVKRDYYEILGVPRNANETDVKKAYRQLALQHHPDRNPGDKAAE
                     EKFKEASEAYEVLRDPEKRELYDRYGHEGISRTGFSGFAGFEDIFSSFGDIFEDFFGF
                     GTGRRRSPGPRKGPDFRYDLTVSFMDAALGKEMEIDIERPETCGTCKGTAIKPGTKRE
                     TCSSCKGTGQISHTQGFFTLRTTCSRCRGQGFFIPHPCTDCRGTGRVKKTKKIPLKIP
                     AGVDSGSRLRVSGEGGEGEKGGRPGDLYVILHVEPHSFFERREDDILCQIPISFVQAA
                     LGEEIEIPTLNGTKKITVPPGTQSGQVFTLRGMGIPHLDGYGKGDQHIQVTVKVPSRL
                     NMRQKELLKEFASLEGKED"
     gene            complement(17336..17941)
                     /locus_tag="VEL68_12620"
     CDS             complement(17336..17941)
                     /locus_tag="VEL68_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HXZ36857.1"
                     /translation="MALFQKKKCDFCEMLHAQAQKVLEGLDALHAWAQGGVDAKRERV
                     KEIEKEADELRRILIEELNQTFVTPFDRDDIFSLSRAIDDVMDYADRTVDEMEIYEVK
                     PNSHVAQMIDILRKSARELSDSIRLIQKYPNIALEHATKAKAAENEMENAYHRALAEL
                     FKGTDTVYMLKMREIYRHLSNAADRGDEAANLIGDIVVKMT"
     gene            complement(18008..19048)
                     /locus_tag="VEL68_12625"
     CDS             complement(18008..19048)
                     /locus_tag="VEL68_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXZ36858.1"
                     /translation="MEFNVLTTAILLIAIVLVFEFSNGFNDCANMVVTPVITGAMEPR
                     KVIFLIALFELIGAWFLGTAVAQTLGKGIVNPQYITAPVIFAAVCGAILWNLGGWYFG
                     MPSSSSHALIGGLVGAVLAGSGGEWIDWIKVAEVLAILVLTPVIGLIAGRYLTQKVLV
                     LFQDSKPSRANPRLKQLQILTSITLALSHGTNDAQKGMGIISLSLIIFHKISPDLMGK
                     IYEPLPEYSFYVPKWVILICSMALALGVSSGGWRIMKTLGTKLYKVRPVHGFSAQACA
                     SIIIYLSSLLGFPVSTTQIVSSSILGAGSAQGFGSVRWGVGKQIFLTWMITIPGSAIL
                     SAVLYLFFKGLF"
     gene            complement(19053..20237)
                     /locus_tag="VEL68_12630"
     CDS             complement(19053..20237)
                     /locus_tag="VEL68_12630"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HXZ36859.1"
                     /translation="MKPNIDQCIKCSICNAYCPVLKATGLFPGPKLAGPDAERFRLQR
                     KSIPLEWLEYCDYCKVCERVCPQNVPIPDLHIKSSLAWKKTRKPPFRDWLLGHAYLLQ
                     KLGSWATPISNWVFGWGFFRWLLDRGLGIARGTPMPAYRRRTFAQRLQSHPQKKGDPI
                     AYFHGCYTNYIDPGLGLAVVEVLGKNGFRVNLPRQECCGLPLISNGFFELASRLGERN
                     VESLRKTIEGGTEVVFSSPSCGMTLKEEYETLLSLRGASLLKDHVFEICQFLLQLYEE
                     GKLDTRFQETQETFYYHVPCHLRALKIGLPALELLSLIPGLRVIELPEGCCGLAGSYG
                     YKQEKYAIAREVGEDLFRAIRSSKARTVISDCEACRLQIGFHTGVKTLHTVQVLRQAY
                     GA"
     gene            complement(20359..21669)
                     /gene="glpB"
                     /locus_tag="VEL68_12635"
     CDS             complement(20359..21669)
                     /gene="glpB"
                     /locus_tag="VEL68_12635"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpB"
                     /protein_id="HXZ36860.1"
                     /translation="MFYDVIVIGAGLAGLMAAEAAQEKGSRVLILAKGMGSLPLTSGC
                     IDGLGYFPESSAAPASSPLENLGELRRANPAHPYSRLGNESILSALARFQNLTLSNGL
                     PYAGEFNASVLIPTALGTYHPTCLVPETMKNGVLSLPGRVLLLGFEGLKDFSACLASE
                     NLNLWRARGKIAPSFRAETLTRVKLEGKALNSLNLARAFDGEEFRERVAREVQPLLKS
                     GEKLGLPAVLGYFSSQSAWGDLQKRLGTDIFEVSLPPPSIPGLRLYNLMRAHLQEKGV
                     RVIIGLSALKPILEKGEIQGIALGEARKSPVYRASAYVLATGKFVGGGLDAQRNRIFE
                     TLFDLPVQYPQNRKEWFSAQLLTSIGQPFNSFGVEVNENLQPADSEGRVIYQNLYAAG
                     GILAHGDSMAEKSGGGVAIATGYVAGQFAAACARKGFATETQRA"
     gene            complement(21755..23290)
                     /gene="glpA"
                     /locus_tag="VEL68_12640"
     CDS             complement(21755..23290)
                     /gene="glpA"
                     /locus_tag="VEL68_12640"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpA"
                     /protein_id="HXZ36861.1"
                     /translation="MKTEVVVIGGGATGAGVFRDLALRGIDTILLEKKEFTSGTSGRN
                     HGLLHSGGRYAVKDPESGRECIRENYILKKIATPCVEDTGGFFLSTPHDPPDYPDRFL
                     KACEEIGIPAEELPAADALQKEPALSPRIVRAIRVPDGAIDPFRLIRANVEESLQLGA
                     KAFPYREVVAIQTEKGAVRGLTAEDSRTGEKFQIGCRFIVNAAGVWAGKIARMAGARL
                     EVVFSKGTLIVLNQRLVNTVINRCRPPSNGDIFVPHGPALILGTTSLATVEIEDPSAS
                     EEEVDLLLSEGEMVIPVAGVTRAIRAYAGVRPLIGAREAEGDTRTISRDFLLLNHGNA
                     VGVKGLFSIVGGKLTTYRLMAERTVDAICKEMGKNAPCVTAEKPLPFPREYSFHDLGS
                     RLSRISGSGSSSEILCECELVTRGEILEEIKKLPFPTLKEVQERTRAGMGPCQGGFCS
                     PRLTALLAETGKITQGTSLDVLKKFLEERWKGTRPILWGPQLKEEQLIQALYSELFNL
                     DHL"
     gene            complement(23378..24157)
                     /locus_tag="VEL68_12645"
     CDS             complement(23378..24157)
                     /locus_tag="VEL68_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HXZ36862.1"
                     /translation="MDALHFLGYLFDLDGTIYLGEKLIPGARETIKRLKSLSKKIVYL
                     SNKPLQTRGEYADKLTRLGIPTRPEEVINSSLVMALWLSREAPGATIYVIGETPLIEE
                     MVRAGFRVSEKAGEIQYVIASFDRTFDYRKLNIALQAIKKGARFVATNPDRTCPVEGG
                     EIPDCAAMIGAVEGTTGKKVETIVGKPSDVMIRVTMDYMGLRPQDCILVGDRLETDMA
                     MGKKAGMAAALVLTGVTARGSLKKSDIQPDYVWESVAEILK"
     gene            24386..25513
                     /locus_tag="VEL68_12650"
     CDS             24386..25513
                     /locus_tag="VEL68_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ36863.1"
                     /translation="MKRKLILGIFLSVFLLLPFSFAQAQSIENELYLITPVSKDVHDP
                     ALNAFAAYAKKKWNMDVKTSAIPKGTPVAYGQILEWKGKPQADIFWGGEGTLFDALAV
                     EGLLEPVKIPKQMWDEIPATIGKPVGLPLKDPKRFWVGTTLEPYGLIYQPKLLNRMGV
                     EIKDWDDLLNPKLKGQIAQCTPDRSSSSHASYEVMLQTYGWEKGWEWLKKLASNTGIF
                     TARSRDVPNVVAKGEFAVGYAVPSYMAFAEVLAGYDVMYVYPKNAYVTPEPMAVLKGA
                     PHPKAAHAFIEFLLTEEGQKLFAELGLYPITPKFKVQGPPGSKQEMTVKFTGGMRSFF
                     DIPVGNVYDEKIAGPKKRIEEVNSYFRKEIAEKHKDIVRSK"
     gene            25613..26692
                     /locus_tag="VEL68_12655"
     CDS             25613..26692
                     /locus_tag="VEL68_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013129380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36864.1"
                     /translation="MNILLKDLVKRFGNLEAVSHISLEIQDGELFTLLGPSGCGKTTL
                     LRLIGGFHRPDGGEIYFGDRPVAPIPPYERNIGMVFQNYALWPHMTIFDNVAYGLKLR
                     KFSREEISSKVSRALQLVNLSGLEKRYPGQLSGGQQQRVALARALVLNPDVLLLDEPL
                     SNLDAKIRIQVRAEIRKLQKELGITTIYVTHDQEEALTLSDRIAVIDRGKMQQLGSPR
                     ALYAKPENPFVADFIGINNLIPGEVREVLDGEKMKVQTEVGLIFCTIERPFKPGDRCM
                     VTIRPETASIIQEEVREKDTNVLPGTVNFASYIGNTIRYDLEMQNGKIFKVDIQNPWY
                     HQILAQGEKVYISIPQQITLGIPIS"
     gene            26708..28426
                     /locus_tag="VEL68_12660"
     CDS             26708..28426
                     /locus_tag="VEL68_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXZ36865.1"
                     /translation="MSPQTETSIAIPQARPFAEMKLGGLLGYLAIWAFFGVFLIYPLI
                     RLFYDAFTTTEGVLTLNNFFDFFTDSYYLKSLVNSLWLGGATVVTTSILGITIAYLLL
                     RYEFPGRRLFSYLTIIPMIMPPMVGVMGFVFIMGRAGTLNIILMDYLGFEKPINFMYG
                     VHGVLLVETLHLFPLMTLSIVDALGKIDLSLDEAAESVGSRGLRKFWDITFPLTTPGY
                     VSGALLVFIWTFADFATPLVVGVDDLLASQAYLNIVQFVDRRLFKMGIVISAIMVLLA
                     ILFLIIAKKYVAIKDYSSLSYSKVERRKISAWGKVLVIVFLVAVLVFSFIPYLGIALD
                     SFGKGWALTPIPVKYTLQYFERVAIETPKFIVNSLLFSGISVLICIIVGVPVAWVMAR
                     TKLPGKDMLDSLTTLILALPGTGIGIAYMRAFRDALPFMDTALIGMWVVIPIVLGVRR
                     LPYTVRGTFASLLLVHKSIEEAAESVGATKSKTFRDVVAPLIWKGIMVGALYSFILAL
                     QEASATLLLVIPGREMMTVGIFNFYIGGSVNEAAALGFILILLGAFCLWLINRLAGSR
                     MGGVFG"
     gene            28553..30061
                     /gene="argH"
                     /locus_tag="VEL68_12665"
     CDS             28553..30061
                     /gene="argH"
                     /locus_tag="VEL68_12665"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXZ36866.1"
                     /translation="MENKKLWGKRFEKKPSALAEAFTSGRDVRGISPADQRLIPYDLW
                     GSRAHVVMLVRQRIIPRRDARLLVTGLREIEKYWQRGSFNLDPSLEDVHTNVEGWLIE
                     KFGIEVGGKLHTARSRNDQVALDMRLYLRDCNLGFVSGLSSLIQTLLRRAKEHRGVIT
                     PGYTHHQSAQVTTLGHIWLSFAGAFSRDVQRFQDWYNRFNENPLGSMTGYGTTFPIDR
                     FLTSKLLGFDGLCENSLDPIQNRWEAEAELGFAVSAMMNHLSSLAQTLILFSTAEFGF
                     VKLDDAYSTGSSMMPQKRNPDTLEVMKAKASAAQGFLASLLSIGKALFLGYNRDTQWT
                     KYLILDLVDECLSAAPLMGEIISSLKISRKEMEARSQAGFIAAPELVEQMVQEWKIPF
                     RQAKGAVEKAIRYAEKEGFKVLPLPLLQKASQEEGLKIKMTKSFARRSQKGYDFISKR
                     VAVGGPSPRSLDRNISTLKRTLQEFHRWLKQKVTEQSRAKMKLARMEQALNL"
     gene            complement(30109..30279)
                     /locus_tag="VEL68_12670"
     CDS             complement(30109..30279)
                     /locus_tag="VEL68_12670"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXZ36867.1"
                     /translation="MIPTKGSISIIVSDETRVARMNILLPILLDEMNRAGFQDWRISV
                     AISNGTHPLIPR"
     gene            complement(30349..31773)
                     /locus_tag="VEL68_12675"
     CDS             complement(30349..31773)
                     /locus_tag="VEL68_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HXZ36868.1"
                     /translation="MNGKERSGTSQIIRGLIAIAIGIIIWYWPAPAGVKKEAMHLLAI
                     FVATIVGLILTPLPMGAVVIIGVMLTGVTETLKVGQILSGFANNTVWLIVAAFLLTRG
                     FIKTGLGRRISYLFIRAFGRKTLGLAYAIAASDLVLSPATPSNTARAGGIIYPIVRGL
                     TSTFGSEPGATARKIGSYLMMMEYQAVIVTSAMFMTSQAASPLLIELAKKTANITISW
                     GMWALAGVLPGLLCLILTPYILYKIYPPEIKETPQASQMAHAELQKMGPMKRSEKILL
                     VVFFMILALWVTSELNKLDATVVALIGVGVMLLTGVISWEDVLGEKGAWDALIWFGGL
                     VMMATGLSQLGFMKWVATSVAGSVTGLAWLPALLVLVLVYFYSHYGFASVTAKVTAMF
                     PPFLAIAMAAQAPPQLTVFLFGMASALGAGITHYGTGPSPIYYNAGYVDLGTWWKLGL
                     LVSIINLVIWFGVGFPWWKALGLW"
     gene            complement(31848..33365)
                     /locus_tag="VEL68_12680"
     CDS             complement(31848..33365)
                     /locus_tag="VEL68_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ36869.1"
                     /translation="MSLQDFTIYDLIARNALIFARRIAWVIDQKRLTFIDFYNNINSL
                     AYNLEIAGLKKGDRIGILSQNCYEFVLLYGAAARLGAIMLPLNWRLSAPEVEYILSDG
                     RPRFLFVGPDFKTTIRGLMPNMGFIEKCYSFGEAEAEFEAFSTLMKGEGKATEVDIQA
                     SDPYLIIHTAAVAGKPRGATLSHGNIIAGNLQHMATMGLREVDSYLHMLPLFHIADLA
                     LSFAVMHAGGKNVLMTKFDPELALQLIEKEKITTLGEFPPMLGMLLEKLQGKNYDISS
                     LKHVGGIDHPSNIERLKKKANVEFWIGFGQSETTGMVTVCAYTERPGSAGKEGPLAKI
                     KLVDDHDREVPVGTPGEICVRGPLVFLGYWKLEEDTRYTFRQGWHHTGDIGRLDENGY
                     LWYVKRKAEKELIKPGGENVYPAEVEKAILEHPEVADVAVFGVRDKEWGEAVKAVCVL
                     KTGSVLTQQGLIDFVASKIARYKKPKYVDFVPALPKTQEGTVDREKVKAEYGKAS"
     gene            complement(33412..34623)
                     /locus_tag="VEL68_12685"
     CDS             complement(33412..34623)
                     /locus_tag="VEL68_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36870.1"
                     /translation="MIYLCENRVFNFIEEPPNNPVLVSMQPILDTPIEKRLSPDDRVR
                     VWACIERKVWEEQSRTEELKIRLVRERIQLEREIQKTLAEKGKIIAEIEEKKRLQAES
                     QRRLEQERRRAEEERQRKVVEEARKVSEEDQRIKFYKSGGKEGLLLPPQPPASAPLPK
                     ITESGVFLVMKEAAIHEEPREQSKVLSRGEKYDLFEVIQSQKDEDGNYWHQVRVGERV
                     ISDKGKKYGWSPEERSFWVKNKLPVWVYLGDFANIANAKAVKIRSEDVHFTSRIALTP
                     QKQTFYEVTYGTNSTFREEILGWVAENNGIRRSTKNKDEMRTLLKDLSKTLWPIRIQE
                     DVLRGFIRQGFTREQVVLSWGKPDHVNTTRTLVGIHEQWVYGEPPFPKSYVYFENGLV
                     KSWEFIRNTDK"
     gene            35619..36782
                     /locus_tag="VEL68_12690"
     CDS             35619..36782
                     /locus_tag="VEL68_12690"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HXZ36871.1"
                     /translation="MVKRSFWAKLLETASARNSVIWLTGVPGSGKSSLCQGFGDIEYF
                     DCTIPKVRMEMEDSEGFLRKLLGKTVVLDEIQEVSNHQEILSIVANQSSPPKIIATSP
                     LSLHSNPNLFDTIGKKITQVWITPMMSQDLLDFGQTNLADRFLRGGLPSFFLNGRKPE
                     REFQEWVNLFWARGLQEPFRVERRNSFNRFIELLFAQSGELFEAKRFINPCGMSHPTV
                     MKYLLALQSSWLVQLIRPFSTRHASEIISIPKTYAFDTGFFCYFRGWQELRQEDFGIL
                     WKHWVLNELNSHLQSRVIHYWRDKGGHEVDFILITKEMGITAIVCQWREKDFQSKNLL
                     AFRRRYPEGRNWIVCGDNVQAHTQTMGKIRLEFMTLKEMSKWTAPLCQIEDNA"
     gene            37231..39105
                     /gene="mnmG"
                     /locus_tag="VEL68_12695"
     CDS             37231..39105
                     /gene="mnmG"
                     /locus_tag="VEL68_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXZ36872.1"
                     /translation="MNTYPTNYDVIVVGAGHAGSEAALACARMGHSTLLLTITLESVA
                     RMSCNPAIGGLAKGQLVKEIDALGGEMGKSTDETGIQFRRLNTKKGPAVRSSRAQSDM
                     QLYHLQMKRVLERQRNLFLRQALVDELIIQRCRAEGVVTNIGERFLSKAVILTTGTFL
                     RGLVHIGLKKFPAGRLGDLPSTGLSECLSGLGFSLGRLKTGTCPRLDGRTIDFSGLEE
                     QWGDHPPKPFSFSHRTVPLRQVPCHITYTNSRTHDAIRSGLNRSPLYSGVIKGTGARY
                     CPSIEDKIVRFAEKDQHQIFLEPMGLDTLEVYPNGLATSLPMDIQVQMLRSIPGLQRV
                     EIMRPGYAIEYDYADPLQLKPSLETKLVENLFFAGQINGTSGYEEAGAQGLMAGINAV
                     LKIRGEEPFILRRDEAYIGVLLDDLVTKGTKEPYRMFTSRAEYRLLLREDNADLRLRE
                     KGRTLGLVSDEDYQEFILKREKIQRAIERIGRTIIHPSAGENDLLRELGSSPIKNPTS
                     LSDLLRRPEISFRHLSVFAPALASTEENVAEEVEIQIKYEGYLRRQSELVEKVVKLEQ
                     MKLPPTVDYASIPGLSTEVKEKLTRVKPLSIGQAARISGITPAALSILMIYLHKASPK
                     "
     gene            39482..40078
                     /gene="rsmG"
                     /locus_tag="VEL68_12700"
     CDS             39482..40078
                     /gene="rsmG"
                     /locus_tag="VEL68_12700"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXZ36873.1"
                     /translation="MTEKHLNDFELYLKELNRWRKKINLVSRKDDREIVLKDFLDSLT
                     LVKYISGMAFLLDLGSGAGFPGYPLKIVLPDLKVFFLEATRKKVFFLKEVQRALGMGG
                     VEVRWTAAKKGIEDLSSRFDFVVSRAFGSIQELAFSGHRFLCKGGMLVAMKGKKGREE
                     LKEGLPSLIEMKLKLAFLESLQIPFLNHERTLIGLIKI"
     gene            40165..40947
                     /locus_tag="VEL68_12705"
     CDS             40165..40947
                     /locus_tag="VEL68_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ36874.1"
                     /translation="MGRVISVANQKGGVGKTTTSVNLSASLAVAEKKTLLIDIDPQGN
                     ATSGLGIGKEKISDRNIYQALIDQANVKELILDTELEYLKIIPSNIDLIGAEIELVAF
                     PDREVRLKRALNDVRGTFDYLIIDCPPSLGLITVNSLTASDSVLIPLQCEYYPMEGLS
                     HLLKTIRLIKDNLNTDLAIEGILLTMVDKRNNLSHQVSEEVRKHFPNLVFNAVIPRNV
                     RLSECPSFGKPIILYDASSRGAESYLELAREIIARNQQGRER"
     gene            41054..41899
                     /locus_tag="VEL68_12710"
     CDS             41054..41899
                     /locus_tag="VEL68_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXZ36875.1"
                     /translation="MTARQALGKGLGALIPEKGIPEADGKKAPQICGIEEIQPNPFQP
                     RKVFSEEQLQELIDSIREKGILQPLLVRRRGNGFELIAGERRWRAAQKAGMKEVPILV
                     KDVSDSEMLELSLVENIQREDLNPIEEAEAYKRLMEQFDLTQEEISKKVGKDRTTITN
                     TVRLLKLPPEIKQSLAEGKISMGHARAFLSLDGIEKQKLLLKRLLTGGLSVRQTESLV
                     KRFRTRNSPVSRKTNPEWSGLMEELQRILGTKVRIVGKRKRGKIEIEFYSLDELDRIL
                     ELLRK"
     gene            41956..42309
                     /locus_tag="VEL68_12715"
     CDS             41956..42309
                     /locus_tag="VEL68_12715"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXZ36876.1"
                     /translation="MNALLGGGTEFEGKLSFEGAVRIDGKFTGEIRGEGMIIIGEKGN
                     FRGEIEAGVVMVRGEAQGTIRAKNRIEAYIPAKIHADLHSPVLVFGEGVIFEGTSHMG
                     AEAKEGDNHPIPGTK"
     gene            42440..42868
                     /locus_tag="VEL68_12720"
     CDS             42440..42868
                     /locus_tag="VEL68_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HXZ36877.1"
                     /translation="MIDLNFTVIFQLAIVLILMVALSNLVFKPFLRVVEERKDWIEGA
                     EKKARALQQRSEELMERYQDSMSAAQAQGASIREEIRKEALSKEAQILQKAMEEGNQF
                     LEEMKGKIREESQKAKAGLRLQAQTLSREMAEKMLGRSIQ"
     gene            42865..43431
                     /gene="atpF"
                     /locus_tag="VEL68_12725"
     CDS             42865..43431
                     /gene="atpF"
                     /locus_tag="VEL68_12725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HXZ36878.1"
                     /translation="MRKFNRLLGLSLWVQFLGVGNLWAAGGGGGFSGEVIWQIIAFLL
                     LGFFLAKMLKKPLGAFLAKRREEIRSSLDQASKKESEAQRLLGDWEKKLEALSQEVRD
                     LQETIRKEGEEERKKIIERAEEEGERVRKQAQIIAEHEVKKARMALKKEMVDLSLELT
                     EKLLKEKIQPQDQERLVHEYIGKMRELR"
     gene            43428..43970
                     /gene="atpH"
                     /locus_tag="VEL68_12730"
     CDS             43428..43970
                     /gene="atpH"
                     /locus_tag="VEL68_12730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HXZ36879.1"
                     /translation="MISLTIARKYAKALLDVGRRERSHELLGKDLDKLWGLLQENKEL
                     RTVLFSPFYPAPRRKAIARSVGESLALSKSVLDFLNLLIERDRMDHFPSIVKSYEELC
                     DEVSNRLRASLASPEILPSQLVGEIQTQLESTTGKEVILSTLEDPSLIGGVVTKIGNI
                     IYDGSLKTQLLKAKENLYKE"
     gene            43977..45494
                     /gene="atpA"
                     /locus_tag="VEL68_12735"
     CDS             43977..45494
                     /gene="atpA"
                     /locus_tag="VEL68_12735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXZ36880.1"
                     /translation="MAVQIKADEITEIIRSQIQGFDRKLDVAETGIILSVGDGIARVY
                     GLEGVMAGELLEFPHGVFGMALNLEQDNVGAVLFGEDKLIKEGDMVKRTKRISQVPVG
                     DELIGRVVNSLGQPIDEGPPVEAKHFVNIEQKAPGIVVRQPVKEPLQTGLKAVDSMIP
                     IGRGQRELIIGDRGTGKTALAVDAIINQKGSGVYCFYVAIGQKRSTVARVVDVLKKYG
                     AMEYTTVVAATASDPAPMQYIAPYAGCAMGEYFRDSGKHALCIYDDLSKHAQAYRQIS
                     LLLRRPPGREAYPGDIFYLHSRLLERAAKMRDDLGGGSLTALPIIETQAGDVSAYIPT
                     NVISITDGQIYLESELFYSGVRPAINVGLSVSRVGGNAQIKAMKQVAGSLRLDLAQYR
                     EMAAFAQFGSDLDKATQAQLARGSRLVEILKQPQYRPIPVEKQILIIFTGTNGFLDGY
                     PESALARYEKDLFEFVDKQYSAVLTDIRQKRTIDAELEKKVKDLLEEFKGKFKAE"
     gene            45501..46409
                     /gene="atpG"
                     /locus_tag="VEL68_12740"
     CDS             45501..46409
                     /gene="atpG"
                     /locus_tag="VEL68_12740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HXZ36881.1"
                     /translation="MPKASLRDIRKRIASVRSTRQITKAMKMVAAAKLRRAQENILAT
                     RPYAAKMLEVLRSLAARTSPDAHPLLYRREPRRIELVVITSDRGLCGAFNMNLIQKAE
                     RFVEEEKTGAESLTLSFIGRKGRDYFRKKKVTIRQEYINLFGKVDYPLAARIGQDLGK
                     SYVADRVDAIYLLYSEFKSAIQQRVVLEKVLPLAPGLLKEAEKGKEISPATAGTAAAV
                     DYIYEPSEVEILGKILPMYVEVQVYRALLESVASEFGARMTAMENATNNAGEMIDKLT
                     LVYNKARQAAITKELIEIVSGAEALK"
     gene            46487..47899
                     /gene="atpD"
                     /locus_tag="VEL68_12745"
     CDS             46487..47899
                     /gene="atpD"
                     /locus_tag="VEL68_12745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXZ36882.1"
                     /translation="MQVGKIVQVIGPVVDVEFSGGELPPIFGALKITNPRIDAREENL
                     IVEVAQHLGDEVVRCVAMDTSDGLVRGMKATYYGEQITLPVGRETLGRILNVVGEPVD
                     EMGPVTAKMRYPIHRPAPEFAEQSTQMEPFETGIKVVDLLAPYLKGGKIGLFGGAGVG
                     KTVVIMELIHNVAMEHGGFSVFAGVGERTREGNDLWLEMKQSKVLEKAALIYGQMTEP
                     PGARARVGLSALTAAEYFRDEEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGELQERITSTKRGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRALVE
                     LGIYPAVDPLDSTSRILDPLVIGEEHYAVARRVQFILQKYKDLQDIIAILGMDELSEE
                     DKIIVSRARKIQRFLSQPFFVAQEFTGTPGVYVKIKDTVRGFKELVEGKWDHLPEQAF
                     YMVGPIEDAVAKAKRLGAMV"
     gene            47910..48326
                     /locus_tag="VEL68_12750"
     CDS             47910..48326
                     /locus_tag="VEL68_12750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HXZ36883.1"
                     /translation="MDKTFLLEIVTPYGLIVSSKVEEVYIPGSQGDMDVLPGHAPLIT
                     SLRVGELHYKQEKEVHYLAINRGFAEVTPARTTILVDTAEPAVEIDVTRARAAQARAE
                     ERLKNLTPRDPSYPQEQEALERARVRLRVAEKAPRE"
     gene            48417..>48574
                     /locus_tag="VEL68_12755"
     CDS             48417..>48574
                     /locus_tag="VEL68_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXZ36884.1"
                     /translation="MGNWFKIGVALGGGGARGLAHLGVLCALERERISFDLLTGTSMG
                     ALVGGMYAL"
BASE COUNT        11692 a        12515 c        13176 g        11191 t
ORIGIN      
        1 gtatccccgg tacttaatca tctccttttt tcggtccaga atgaatacat acccttcttc
       61 gtccattcgg gcgatgtccc cggtgtagag ccagccgtcg cggagggcca gccgggtatc
      121 ttccgggtgc ttccagtacc cctggaacag gttagggccc cggactacta gctctccggg
      181 ttcccccaac ttcatttctg tctctccctt atcccagtcc atgatcttat cttccatatc
      241 gctcaccgca atcccaaccg atccttcttt gggacgatcc ggtggattgg aatgggtggt
      301 gggagaagcc tcgctcaaac cgtaatggcg ggcaatgggt acccggaact tctcctgaat
      361 cgttcgggcg atggccggaa ccaggggagc ggcaccgatc cagatgtacc gcagggagga
      421 aaggtcgaat cgttctagat cgggcacgtt cagaaaggcc aacaaagccg gctgaacgcc
      481 gaagatcatc gtgacccggt atttttccat gaggcccaaa cactcttcgg catggaagcg
      541 ggacatcacc acctgagtag cccccaggta gatggagccg cagaggaagt aggtgaaccc
      601 ataaatatgg tggaagggga ggaaactgat gaagatttca ctttccctgg cttccacggc
      661 attcatggac tggattgtat tggacaccag attgaagtga gtgatcataa cccctttgct
      721 taatccagcc gtgcccgagg agtaaggaag agcagccagg tctgccttgg tctgaatcgg
      781 gggaagggag agggacaggg gggcagagta tttttgaagt agattttgca gagagaagac
      841 cctcctcccc gagggttctt ttccccgggt gaggataacc ttttcaagag agggcatttg
      901 gggaagaatt ttctctacta ccggaaaaag gtccttttga atcagaatca ctctgctttc
      961 agcatcattg acctggtttt tcacctctcg ttcgcgaagc gtgggattca tcggagagac
     1021 aatggctccc atcttcatag cggcgtaaaa gcccacgaag tactcccaac cattgggcag
     1081 gaaaaaagag acccggtcgc cttttcggat tccccagtcg aggaaagcct gggccagggc
     1141 atcgctttcc cggtccattt ccagaaaggt gagtaccctc tctccatgaa catcgatgac
     1201 cgcaacctta ttcggaaggc gccccacagt cttcttgaga aaatgggaga ccgattcctc
     1261 tggatagtca agagagggac gccgcatctt ggaagtactc atgtccatac aatctgcaaa
     1321 taggaggaga acagcttgct ttctcgcctt caaggagaaa aagaatccgg ccctagagat
     1381 tttaaattcc cgattatggg aaatggatag tcggatgtcg actggatgga cccaccccag
     1441 atgggccaca tagtccggtg gtcgctttta cgaatagtca ttggacccac gggagtagga
     1501 aaggttaaat cttcgagggc ccggaaagct ttttccgaat ccagggaccc ggctttttgg
     1561 attgctttaa taagggcatg aatgctgata tatccgctta gggccgccaa gctggggtaa
     1621 gaattggtat tgtttcggta tttggaaaca aacaccttgg tttccttcca gtccagagcc
     1681 caaaagggaa aagcgctgat cccccagact ccctcgggga ctttttttct catgccaacc
     1741 agcgaatcca ggtttccaga ttcgagttcc aggtgaatga tttttttaaa atatccctct
     1801 ttattggcca gctgatagaa ctccagccag tccttcactc ccaggtggct caggcatact
     1861 tcggtattca acgccatcag ggtccggagg tagggacgat agtcatcgac gcgcggaggg
     1921 aggaacgtct cccccagccg ctgggcctca ggattctgct ttttcatcgc ctcccagaag
     1981 tatcccaccc aatttctacc cagggctgag ttacgagcca gaaggtagaa gcgctttttg
     2041 gaaaattgag ctgcagtccg gacggcggcg cgctggaagg cctcgagcgt cggagtgatc
     2101 cagaaaaggc cagagggctc gtccccggtt gagactgcct ccataaattc aattgggaaa
     2161 gccaggatgg gaatttggtg ttcttgagcc aatcgggaaa ctggaatgat agcttctcgg
     2221 tgcaaggctc cgatcagaaa ggaaggccgc tgttcgaaaa aaaagtcccg gaggacgctc
     2281 tgttcccttc tatcgaggaa agtccctctg cgcgccaaga gaagcaactg aatcccctgt
     2341 tttccttcga cagcgtttac ttcggcgacc gcaatctccg ccccccgcgc ataagaggaa
     2401 tcggctccgg ttccggaatc ctgccaagga atcaagaggc cgatttttaa cagttccgca
     2461 tgcgttgtca ggggaaagaa catgggcaag agcaaaaagg aaaagccgat gattcccaga
     2521 aaaaagtgga atttcaaggg ggaggctaaa ggagatgagc cgcagcggtt attcgaaaag
     2581 gcgagctgcc ggggcgtacg gaatgggctc tcgattccga tctgattttc ctccttttaa
     2641 tggaaaaaat aatgggtttt gaatcatgaa ggaatacgga tttagggcga aagccagaca
     2701 aaaaagaagc actgcactac gataacctat attagcaggg aagtagaata aagggaacgc
     2761 aaaattgaac gtctgtttac gccacagatc attgggcggc atttaataat tgacgaaatg
     2821 gaaaatatac attaatctaa ttctttaggc tctaattcct ttaagcaact atgtttgatt
     2881 ctcttagcga caagctgagc acggtcttca agaagcttcg cggacacggc aagcttacgg
     2941 agcaaaacat ctccgaagcc ctaaaagaag tccgcctggc cctattggag gcagatgtaa
     3001 atttcaaagt tgtcaaggat ttcatcgaac gcacccagac tcgtgccgtt ggacaggaag
     3061 ttctgagaag tttgacccct gcccagcaag tggttaaaat cgttcatgaa gagctcatct
     3121 ccctcatggg agggtccagc agcgggctta acctgtctta caagcctcca attcccatca
     3181 tgctcgttgg gctgcagggc tccggaaaaa ctactacggc ggcaaagctg gggaagtttc
     3241 tgaaggacaa aggccggaag gtccatctgg taccagccga tgttcgccgc cctgcggcgg
     3301 tcgagcagtt gaagaaactg ggcgagcaac ttgcaatccc ggtttatcct accgatcccc
     3361 aagagagtcc ggtgaaaatc tgccgcaagg ctctccagtg ggccgatgaa gagagcgggg
     3421 atgtgttgct cattgatacg gcaggtcggc tgcatatcga tgaggccttg atgaaagaac
     3481 tggaagaaat caagaaggaa gtcacacccc gggagattct tctggtggct gacgccatga
     3541 ctgggcaaga cgcggtcacc gtggctaaaa agttcaatga ttccctcgat cttcacggcg
     3601 taatcttgac caaattggac ggagacgccc ggggtggtgc ggcgctttca atcaaggcgg
     3661 taaccggaag acccataaaa tttataggaa tcggagagaa actcgaggcc ctggagctct
     3721 tccatccgga tcgaatggcc tcccgaatcc tgggaatggg ggatgttctc tccctgatcg
     3781 agaaagccca ggaagccatc gatgagaaga aagcccagga gctccagaag aagctgctaa
     3841 aggattcttt taccctggaa gattttcggg atcagctgca gcagattaaa aagatgggtt
     3901 ccatcgaaga aatcctgtcc atgattccgg gaatgggtcg tcttaaggtc cccaaagacc
     3961 tgcagggaag tgagaaagag ctaatcagag tagaggcgat cataaattca atgactcgtc
     4021 aggagaggca ccacccggag attttgaatg ggagccgccg tctgcggatc gctaagggaa
     4081 gcgggacgac cgttcaggat gtgaatcagc tgctcaagca ataccttgag accaagaaaa
     4141 tgctccgaac cttccggaaa ggcgggatga aagggctacc ccgaggcctt ttcccctgaa
     4201 gggcggtagg tcatggagga aggggagcgg cttggatatc cggatttccg gaatcccctt
     4261 tgaattgagg aaggaccttt cagggtctca tggagggtgc cgatcagatt tgagccgaag
     4321 tgacttttct gaagtaagaa gattctgacg aagagaaagg aagaagtttt atggcggtaa
     4381 ccattcgatt aacccgtatg gggagtaaga aaaagccctt ctaccggctt gtggcagccg
     4441 attcgcgctt tccccgagat ggtcggttta tcgaggtttt gggaacctat gatcctaaaa
     4501 ccaacccccc ggccattaaa ctgaatcggg aaaaagtgga ggcctggctg aaccagggag
     4561 ccagtgtttc ggcgacggtg aagagtctcc tgaaaaaggg aggcgtctcc ctccggccaa
     4621 cccaaaagtc aacccctgcc aatcaggagg gtggaccccg ggcggaatcg ccggcctgaa
     4681 ttgggatgtc catccttctt gaggaattcg tttgctcgac cgtgcgcgcc tgagaatgag
     4741 atggccaaag ggccttactc acagggaaag gggggcgagg ttctatggat atgaaagaat
     4801 tgatcgcgta cattgcgaaa gccctggtgg acgcccctga ggctgtggag gtctcggagg
     4861 tggagggaga gcaaacttcg gtaattgaat tgaaagtcgc caaaggcgac ctgggaaagg
     4921 taatcggaaa gcaagggcgg acagctcggg cgatgcgcac tattttgaat gccgcctcga
     4981 ccaaaattaa aaagcgggcg gtcctggaga tcattgagtg aacctcccat ggaagatccg
     5041 cttctatggg tcggccggtt agtcaagacc cagggcatca aaggagaggt cagagtttcc
     5101 tcgccgggaa gagaggccct ggcgcaatcc cgggggaaag tggtttacct ggaggacccc
     5161 aagggaaacc gaaaggcgtt cacggtagcc gcgagcaggc ccttgcgaga attgaccgtt
     5221 ctcgcctttg acgagatcaa aagcattgaa gaagcccaga acctgatcgg ttgttccgta
     5281 tacgtaaaca aggccaatct gagtcccttg cccccggatg aattctactg gtaccagcta
     5341 cgggggctcc gtgtagagac cggagaggga aagtttttag gaattctgga ggaagttttt
     5401 tccacgggaa gtaacgatgt gttcgtggtc cgcaaggagg ggaaagaaat tctcctcccg
     5461 gccacggatg aagtggtagc ccaagtaaac ttgcaggaga aagttatgat cattcatcct
     5521 ctggaagggc tgctgccgga agatggtctt tgatattctg acgatttttc cggagatctt
     5581 ccgatccccc ctgtcggaga gcctgataaa aaaagctctg gacaagggag tccttcaggt
     5641 acgcatctgg aatcttcggg attttacgga ggacaagcat cggacgacgg atgattaccc
     5701 ttatgggggc ggagccggca tggtcatgaa agccgatccc attttccgcg ccgtagagga
     5761 aataaaggct aataacccgg acgcccgaac cctccttctg accccccagg gggagcgatt
     5821 tcaccaggga ttggccaaag gtctaagcga gcagaagcac tggatcctga tctgcggacg
     5881 atatgaaggg ctggacgaac gagttcggct gggagtcgta gaccgggaga tttccatcgg
     5941 agattacgtc ctcaacgggg gcgagattcc cgcgctggtc ttcctggaag ttatcagccg
     6001 gtacgttccc ggttttctgg gcagcgagca gtccgtagaa gaagaaactt tttcggacgg
     6061 acttctggaa taccctcagt acacccggcc gcccgtcatt cgtggcatgg aggttcccga
     6121 aatccttctc tccggaaacc atgcggagat caagcggtgg agacgaaggg agtcgctgaa
     6181 gcggacgtat ctgcggaggc cagacctttt agaaagggct gatttatccg aagaagacct
     6241 tgagtacctg gctgagttaa agagccagaa agcgagggaa taaaagtagg ggcgggttta
     6301 aaacccgccc ctacaccgac catgaagatc tttggacagg gcatttttgt tggaaagcta
     6361 tcatttcgta tttggaggtt gtcatgagag ccattgaaca gctggaaaag gaacagatgc
     6421 gcatggacat acccgatttt cgggctggag atacagtgaa ggttcatgtg aagattaaag
     6481 agggggaaaa agaacggatc caggtttttg aaggaattgt gatccgcaaa cggcagggtg
     6541 ccagccgggc cagcttcacc gtgcgaaaag tctcctatgg ggtgggtgtg gagcggatct
     6601 ttcctaccca ttcgccggcc attgaccaca ttgatgttgt gcagagaggg aaggtccgcc
     6661 gggccaagct ttattacctc agggcactga ggggcaaggc cgctcggatc cgcgagcaga
     6721 gagaaaatta gttttcatgc tctcatttca gatttgagat ttcagatttc agcttggatc
     6781 ccaatttttt gaaatctgcc gtcgacaatc tgtaatccta aatccgctgc ttatgggatg
     6841 ggaaccattc gaggtgaaag ctcgaagcga gggctttcag gtcatcgccg ggctggatga
     6901 ggccggcagg gggccactgg ctgggccggt ggtggccgca gccgtcgttc ttcccccaac
     6961 ccgcaacctg aagggggttg cggattccaa gacgttgacc gcggaccggc gggagaaggc
     7021 tttcttcctc atccaaaaaa aagccctagg aatgggaatt ggcatcgtgg aggccgagga
     7081 aattgatcgg ctgaacatcc tccgggcctc cctgaaagcc atggaactgg cgctgcaagg
     7141 cctttccctc tccccggatt gtctcctcat cgacggactc cacacgctca accttccact
     7201 gaaacaacag gccattacca aaggggacgg aaggtgtctc tccattgccg ccgcctccat
     7261 cgtggccaaa gtcacccggg accggctgat ggtggaatac cacgagaaat atcctcatta
     7321 caatttcgcc cggcacaaag gatacgggac ccgggagcac ctccaggcca tccgggagca
     7381 cgggtgctgc cctatccacc gccaatcttt ccgcaccatt taccaacttt ctctcctatg
     7441 acccaagatc ggctctccct cgggaaaaaa ggcgaggaat tggccatcgc ccagctcgag
     7501 gcgctgaaat acaaaatcct ggaacggaat ttcaaatgtc ccctgggcga gatcgatatc
     7561 attgccaggg ataaaagtac cctggtcttc attgaagtta aaacccgggc gacaaaagat
     7621 ttcggagggc cggcggccgc agtccacgag cggaagcagc gccaactctc taaagtcgcc
     7681 ctggtttatt tgaatcaaaa gaaactcttc aatatacccg cgcggtttga tgtggtggcc
     7741 gtggaactca tccctcccta ccccagaatt gaggtaatcc gcaacgcttt cgatctctgc
     7801 tatgaatgat tcgggaggga aaaatcgggt ccaggttgaa ggaaaaaaat ctggcccctg
     7861 gccatgaatt ttagggggaa agacccggct ttccaggtca tttatcttta ggtcaatatt
     7921 tatccaggcc gtacatccgt gctgcatttt cgtgcaagat cattcgcccc gccttttctg
     7981 cctgtttgaa agttagcaga ccgaatctaa ccgcatcacc caaagcctct gccagagcga
     8041 gcttggcggt cttggccgcc agccagggga tttccggggt atcgtggccg ccgcttccaa
     8101 ctataatctt ggagagaggc gcgattccaa tcacctcctt gtagcaatcg accagacgca
     8161 gggaaaccca tggtgtggtc cagctcatgt ccacccatac atggggcagg gagtgagcca
     8221 gctccgccgc atgttgaagc catggataag cggcatgaag aaaaacgatc ctggtttgaa
     8281 ggaacctggg ctgtctgatg agggggacca acaagaaggg gtcagcattc gaaatagggc
     8341 cgtcccaaag tccgcccgtg cacccgctat ggaggtgaac gggaatcctc aaatcctgac
     8401 actgcagaag cgtgtgggtg aaggctgcta cataaagctc cttgtaggca tcagaatcct
     8461 tagctttggc ggccggaaag atcgcttccg cttctgcatc cgagaccggc tcaactccga
     8521 aaccaacctg ttcagccaga tggcatttta ccccgcaaaa tccctcctgt tttgctgccc
     8581 cgctcagccc ttcctggtat tcacgtagga attctttata ggaatcggtt ctctcgagca
     8641 gattatcgat aagcgggtcc atctggaaga gtctcatcac ttttccgggg attaaatcga
     8701 ggagggggtc cttcatggac agtccagcat ccacaaccga tccgacgatc cccgcgtctt
     8761 catatagtaa tctgacatag gccggaaagt ttttggaagt tctccggctc cgttcggcag
     8821 cgactgcctc caactcatct ggacagccta atagcttgga tagctggcaa accatggttt
     8881 ggataacccc catatgacgc aagtgatacc ggagttcatc cgtagttccc cagagtttag
     8941 cctttttgac cccctctttg gggatatcgc ccattccatg gaaaaaaacc ttggcgagcg
     9001 aatccgcatc cagggtaacc ttctcgaggt caaagggatg gctgtggtta tcgatgatcg
     9061 ggacctcact gaaatcgagc atactcatgc acctcttttc catttttggg gggttaaagc
     9121 cattgtgaaa tcctggaatc ggatttgtca attgggattt tgatctttga tatttgaatt
     9181 ttctccggta ttctatcggt aatggaccaa gacattcttc gagatcagga aggcttccgg
     9241 cagcaggtta ttgatgccct ctccaaggag cctgtagatt atgtggaaca gtttgccttt
     9301 atcaagcgtg agaaggagac gcaggaaaac ttttccggag gaggggttct cttgccgctg
     9361 ttttttgggg aaggggggaa ttggggagaa gcgggacccg gcgagtatgt cttccttctc
     9421 aacaagcgct ccaaacaggt ccttcagccc ggagacctct gtgcgcccgg cggaggaatt
     9481 catccttttt gggattcctt tacccaaaaa atccttcaat tcaaactcct gcccggagtt
     9541 ggaggacccg gattgaaact ggccaagaaa agagggaaag aggtttacga aaagatactc
     9601 tttctcctgg ggaatgccct gcgggagagc tgggaggagt tacggctgag cccgtttaat
     9661 gtggaatttc tcggcccgct gccgacctac tccctttcaa gccggagatg gatcatcttt
     9721 cctcttgtgg gaagagtaaa acgctcttgg caacctaaac tcagctggga agtggaaaag
     9781 atcgttccca ttcctctatc cgcttttttc aaaccgggaa actacgccgt ctattccctg
     9841 gaagtccccg aaaagttggt cgcacacggc atccccagtc cttgggagtt tccctgtctg
     9901 gtccatcggg agaagggcga agaagagatc ctctggggag ccactttcaa agtgatccag
     9961 actttcttcc agatcgtctt aggattctct ttgccctacc cggatggcgg tcaagtcatc
    10021 cgccggccgc tggcttcaaa ctatttgacc gggagggaag agttgtagct caaaaccgaa
    10081 tgcccgaaat tggtctccct ttttcgcttg acagaaaaag agcatagatt atagtctttt
    10141 tgggtaaatt tcattcatcc agggaaccgg atggagactt tgtctttttc tctgagcgag
    10201 attccctgaa aggtcttgga gaatttctct ccggtaaagg agattccaga aggagccgac
    10261 ttcaaacctt catcggctgc cttactttcc gatcgcggtc agaaagaaca aggatccctc
    10321 ccttgtctca gccaccagcg gatatgcttg ggctaaggac aagcccttaa gggaaaggaa
    10381 atttcttatg gatgccattg ggtccaccat cgtcattcag gtccttcatg gtttggccta
    10441 cggaatgctc ctctttttgg ttgcctccgg ccttaccctg gtctttggca tgatgagcgt
    10501 tctgaacctg gctcatgcct ccttctacat gctgggggcc tatttctcct accagatgct
    10561 caagtgggtc gggaatttct ggattggcct gatcctatgt ccgattctgg tgggcttgct
    10621 gggaatcctg atggaacggt ttctcctccg gcgggtccat gcgagtggcc acgtgcagga
    10681 attcgttcta accgctggaa tcgcctttgt ggtcatggaa gtggtgaagt gggtctgggg
    10741 gacagaacct ctccccgtgc ccatccccgc cgctctatcc ggctcgctca atatcgtggg
    10801 aaacgtatac ccggcctacc gccttttcat cctcttcttc tccatcgtgg tcctggcctt
    10861 tatggcctac atcctgatga agacccgtct gggcattacg gttcgcgcag ccgttgcaga
    10921 ccgggacatg gtcagcgccc tggggacgaa tacccccttc gtcttcatgc tagtcatggg
    10981 catcgggacc tggctggcag ggctggccgg ggtcatcgcc gggcccttca tcagcaccta
    11041 ccctggcatg gccggagaga tcttggtgga ttgcttcgtg gtcaccgtga ccggagggct
    11101 tgggagcctg gtcggggcgg ttgttgcttc ttttctcatt gggcagctct cctcgttcgg
    11161 gatccttctg attccccgtt ttgccatcgt ctttgccttc ctgctcatgg ctatcgtctt
    11221 gatcattaag ccctccgggc tcttcggaga aaaggggagg gtctcatgaa aacgaaaaag
    11281 ggattgctgc tggccgtcgc ctttgcggtc tttttgatcc tatttccttt ctttgccccg
    11341 aaattccagt tgcgcatgtt cacagaaatc ataatcctta ccctctttgc catgacctgg
    11401 aagatgatgc tcaatgaggg aggaatgttc tccttcggcc atgcggtcta ttttgggtta
    11461 ggggcttacg cgtccgtgct cgggtggctg catatccccg ggctctcttt tttgagcggc
    11521 attctcctgg gggccctggt ctcggccctg gtggctttcc tcctgggtgc cttcctggtg
    11581 cggatgagcg ggacgtattt cgcccttctg accctggctt tcaatcagct catctgggcc
    11641 atcgtgtgga aatggcgcga tgtgaccggg ggggacgacg gattggggaa gtttcccaaa
    11701 ccggacctct tcggcctggc catgaaaaat ccggtgagct tctattatgt ggccttggtc
    11761 atcatcggta tcggcctcta tatctgctgg tacttcacca aaacccccat gggcaacgtg
    11821 gccctatcca tcaaatccaa cgaggagcgg gccaagttcg tgggattcaa cgtgcaggcc
    11881 acgaagctga tctttttgac tctcctgggt ttcctggcgg ggatttcagg ggctctctac
    11941 gcccagttcc aggaattcat cgccaccagc gtgatcgatg ggatgatgtc caccaacgtc
    12001 ctcttcatgg cttacatcgg cggcaccggc tttttctggg gccctatcat cggctcgggc
    12061 attttcgtct acctttcgga atatctgagc agcttcacgg accggtggga gttgatcctg
    12121 ggcctgattt tcatcgccgt tgtcctcttt gccccccagg gaatcatggg ggtcgtgcgg
    12181 aagaaaaaat aaggggggga agccatggcc gaaacggccg ggaaattggg gaatcctatt
    12241 ttgcagctac aaggcatcta caaggatttt gaggggctgg aagtcctctt cggaatcaat
    12301 ctggcgatcc agcaaggaga acggcatgct atcatcgggc cgaacggggc ggggaagagc
    12361 accattttca atatcataac tggaaagtac ctgcccagca aaggcaacat ctttttcaaa
    12421 ggacaggatg tcaccgggac ctccccttac aaactgaacc ggcacgggtt ggcgcgctct
    12481 tttcagatca ccaatatttt ccggaccatg accgtttttc agaatgtgcg caatgcgatt
    12541 ctttccagga ataaaatccg ttacaatctg ttctcccgtc tggatcggat gatgaacatc
    12601 caggaacaga ccgatgaggt cctggagcag atcgggctgc tggatcgaaa ggacgtggtg
    12661 gcagggctcc tttcctatgg ccagcagcgg gccttggaga tcgggctgac cattgccacg
    12721 gagcccgagc tgattctcct ggatgagccc acggcgggca tgtcctccga agaaacccgc
    12781 gaggcggtga agcttatcgg gcgggtcacc caggggagaa ccttgatcat cgttgagcac
    12841 gacatggagg tcgtcttcag cttggccgac cggattacgg ttatctatta tggagagata
    12901 ctggcctcgg gcccccccga tgaaatccgg cagaaccaaa aggtaaaaga cgcttatctg
    12961 ggggaggaaa aggaggaata gcccatgtta ctggagatcc aaaacctgaa cactttttac
    13021 gagcagagtc acatcctcca gggaatctcc ctgaatatcg ataccggaga gatcgtatgc
    13081 ctcctaggac ggaacggggt gggtaaaagc accactctta agtccatcat cggcttagtc
    13141 tccccccagt ccggggaggt tctcttcaaa gggcggaacg tggccggtat gccgcccttc
    13201 gatatagcca agatgggggt gggctacgtc cccgaagatc ggaggatttt tcctactctg
    13261 agcgtcaggg aaaacctgat catggggatc aaacccggcc agaagagcaa cggagacggc
    13321 tggagcgtgg aaaaggtata caaatacttc ccgggtttgc aggcccggga caagcagaag
    13381 ggcggcttcc tctccggtgg cgaacagcag atgctgacca tcgcccggac cctcatgggc
    13441 aacccggaag tcctcctggt tgatgagccg accgaaggac tggctccgct gattgtaaag
    13501 acagtggagc aggtgatcca ggacatccac cagcataaca ttcccattct tctggtggag
    13561 cagaacatgc gtgtggccct tcgcctcgcg gggcgaatct acgtcatgtc gaagggcaag
    13621 attgtcttcc atgggacgag tcaggaatta aaagaggccc acgaggttcg tgagaagtac
    13681 ttagaggttt aagggcaaag gtctaggaca ccagcggccc gctggggatg aaggttttcc
    13741 cgctggccgt tccaaaaaac ccaagagagg agagaaagaa tggccaaaag agtcctttta
    13801 tttgtttttt taatagcttt attcacctcg ccgggctggg ctcaggacac ggtaaccatg
    13861 attcagttgg accccctgtc tggacccttc aaagatgtgg gtgacctgtc cattgccgga
    13921 gttcagttct tggtcgatga agtcaatgcc gccggcggcc ttttggggaa gaagatcaag
    13981 tatatgcccg aggacagcca gctcaagccc gacgtggcgg tgcgtaaggc caccaaagcg
    14041 atcatggaag ataatgccaa aatcattctt cagctcagct ccaccgcagt ggcccgggcc
    14101 ttgatggatg tggcggagaa aaacaaagtc attttcgtaa ccctgggggc cgagtcggat
    14161 ttcctcactg gcaaggattt caatccatat tttttccgga cctgttttac caccgcgaat
    14221 cgttccaagt cctatgcgga attcttcaag accaaaccct ggcggaaggt ttacctgatc
    14281 aacatggact atgccttcgg ccatgccgta gccgacgact ttaaggtcac catgaaaaaa
    14341 gaaatcccgg atctaaagat cgtgggagaa gattaccatc ccctggcgac taaggacttc
    14401 gggccttaca tcagcaagat cctggcttct ggcgctgaaa tcatcttcac cggaaactgg
    14461 ggaacggacc tcgaagtcct gatccgccaa ggaacccaaa tgggggtgaa gacccggtac
    14521 gcaacttatt tcctggatga ccgagtccgt ctctccaaca ttggcgagtt cgccgtgggg
    14581 agctttgtaa acagcaccta tcttcccacc atcgacaccc cgcagaataa aggctttttg
    14641 gaacgatggc acaagaagaa taaagacacc aaagacccct ggcccaccgc ccaaatcggg
    14701 tacggctata acggggtgag cttcctcttc gaggcgatca aaaaggccaa gtcctttgat
    14761 gccgatgcgg tcatcaaggc ctgggaaggc atggaatacg gaagcctggt gggcaagcag
    14821 atcatgcggg cctgcgacca ccagatcttg atgcccgggc ccgtcggcga aatccaggct
    14881 aaatccagcc tgttcccttt ccccttcccc ggagcgccgc tgatggttcc tatggacaag
    14941 gtggcagtcc ccttcaaaga aacagggaac ccgagatgca aatgaaggat taatatttgt
    15001 tatgggatgc tcgtagggat gggaggcctt gttctttcca tccctttttc atgcaggaga
    15061 aaggagaaaa aaggagaata tggccatcaa aaaaatcagt gtgatcggtt tagggacctt
    15121 gggaacccag atcgccatcc aggcggctta ctatggttat gaggttcgcg ggtatgacca
    15181 ggaccctcag accttccaga aaaccatcca gaagattaaa gggatgatga aatttctggg
    15241 caaaaaccca accatgggtg cggaagagtg ggagaaggga gccggcaagg tcaaactggc
    15301 caaagatttg gctgaggcgg taaaggatgg agaattggtc attgaggtgg tgccggaagt
    15361 cctcgaactg aagcgaaaag tctggggtca gatcgattcc ctggctccga aaggggctct
    15421 tttagccacg aacagctctt ccattcccgt ctcccggatc gaaagcgcca cccaaaggcc
    15481 ggagaaatgc atgaacatcc atttttacca gccggcagtc ggctggaaca tcgtagatat
    15541 gatgggcgga acccaaaccc ttccggagac tctggagacg gccaagcaat tcatccggtc
    15601 cattcagtgc attccgctga ccgtgaaaaa agaaatcctg ggcttctgct tcaatagcgt
    15661 ttggcgggcg atcaagaggc aaacccttta tctatggggt gagggatttg tcgactttcg
    15721 ggatattgac cgggcctgga tggtgatgtt tactccgact catgggccgt ttttcctgat
    15781 ggacctggtt ggcctggacg tggtctacga catcgaaata tcctactaca acgaatccaa
    15841 ggaccccaag gattatccgc cgaaggcgct aaaggacatg atcgagcgga aagaactggg
    15901 ggtgaaaaca ggaaaaggtt tttacacgta tccgaacccg gagttcggcc ggcccgagtt
    15961 tttgaagggt aaatagcggg cgcgatggga aatgatggga gaaggaaggc cggcatatag
    16021 ccggaatata gggatggtgg aaggatggaa tttgggctta tgcaaattgg cgtaaaaacc
    16081 ggcattccat cctttcaccc atccatcaat cctcttttcc ctcgagtgaa gcaaactctt
    16141 tgagcagttc cttctgacgc atgttcagcc tggaaggaac tttgaccgtc acctggatgt
    16201 gctggtctcc cttcccatat ccgtccagat gagggatgcc cattccgcgc agggtgaaga
    16261 cctggccgct ctgggttccg ggcggaacgg tgattttttt ggttccgttg agggtcggga
    16321 tttcaatctc ttctcccagt gcggcttgga caaaagaaat aggaatctgg caaagaatat
    16381 catcctcccg tcgctcaaag aaggagtggg gctctacgtg taaaatgacg taaagatccc
    16441 cgggccggcc tcccttttcg ccctctccgc cctctcccga aactcgtaga cgactccctg
    16501 aatccacccc tgccggaatc ttcaaaggaa ttttcttagt ttttttcacc ctccccgttc
    16561 ccctgcaatc cgtgcaggga tgggggataa aaaacccctg tccccggcag cgggagcagg
    16621 ttgttcgcag ggtaaagaac ccctgagtat gggaaatctg gccggttcct ttgcaggagg
    16681 aacaggtttc tctcttggtg cccggtttta tggcggttcc cttgcaggtt ccgcaggttt
    16741 ccggacgttc gatatcaatc tccatctcct tgcctagggc cgcgtccatg aaggagacgg
    16801 tcagatcata gcggaagtca ggccctttcc ttggcccggg cgacctgcgg cggccggtgc
    16861 cgaatccgaa gaaatcctcg aagatgtcgc caaaactgga gaagatgtct tcaaaaccgg
    16921 caaagccgga gaatcccgtc cggctgatgc cttcgtgtcc gtaccgatca tagagctcac
    16981 gcttctccgg gtctcggaga acctcgtatg cttcggatgc ctctttgaac ttttcctctg
    17041 cggctttatc cccgggattc cgatccggat gatgctggag ggccagctgg cggtaggctt
    17101 tcttgacgtc cgtttcattg gcgttccggg gcacgcccag gatttcgtag taatctctct
    17161 tcaccatttt agaaagtgct tcctccttaa aaatattaac attctcgctg ttaatgtcaa
    17221 gttggagggg ttggagaagg atctgaagcg gcgcttcttt tccagaaaaa aaataggggc
    17281 ggttcacgaa ccgcccctgc taggccaata aagagattgg aaattttgag gaaagtcaag
    17341 tcatcttgac cacaatgtcg ccgatcaagt tggcggcttc atcccctcgg tcggcggcat
    17401 tggagagatg acggtagatt tcccgcattt ttagcatata aaccgtatcc gtacccttga
    17461 aaagttcggc cagggcccgg tggtaggcgt tctccatttc attctctgcg gccttagcct
    17521 tggtggcgtg ctccagagcg atgttgggat atttctggat caggcggatg gaatcgctca
    17581 gctcccgggc cgacttgcgc aagatgtcga tcatctgggc tacatggctg ttgggtttta
    17641 cttcatagat ttccatctcg tccactgtgc ggtcggcata atccatcacg tcgtcgatgg
    17701 cccgcgataa agagaagata tcgtcccggt caaaaggggt cacaaaggtc tggttgagtt
    17761 cttcgatcag gatgcgtctc aactcatccg cctccttctc gatttccttc accctttccc
    17821 gcttggcgtc cactccccct tgggcccagg catgtagggc gtcgagtcct tcgaggactt
    17881 tttgcgcctg ggcatgcagc atttcacaga aatcgcattt cttcttctga aataaggcca
    17941 tttcgggttc cctgatttga tggagagtgc atataatatg ccagatatga ttttttgtcc
    18001 agaaaaatca aaataggccc ttgaaaaaga gataaagaac ggcggagaga atagccgacc
    18061 ccggaatcgt aatcatccag gtaagaaata tttgtttccc cactccccat cgcacagaac
    18121 cgaatccctg ggccgaccct gcccccagaa tggaagaaga gacaatctga gtggtgctca
    18181 cagggaaacc aaggagcgaa gaaagataaa tgatgatcga agcgcaggcc tgagcgctga
    18241 acccgtggac gggtcttacc ttgtacaatt ttgtccccag ggtcttcatg atgcgccatc
    18301 ccccggaact cacccccaga gccagggcca tagaacagat taagattacc catttgggga
    18361 cgtagaagga atattcgggg agaggctcgt aaatcttccc catcaggtcc ggagaaatct
    18421 tatggaatat gatgagggag aggctgataa tgcccatacc tttttgggcg tcattggtgc
    18481 cgtgactcag ggccagggta atcgaggtca gaatctggag ctgtttaagg cgggggttcg
    18541 cccgactggg ctttgaatcc tggaaaagaa ccaagacttt ttgggtcaaa tagcggccag
    18601 caataagacc gattaccggc gtaaggacga ggatggccag gacttcggcg accttgatcc
    18661 aatcaatcca ttctcctccg gagcccgcca aaacggcccc caccaatcct ccgattaggg
    18721 catgagagga actggaaggc atgccgaagt accatccacc cagattccag aggatcgccc
    18781 cgcagaccgc cgcgaatata accggcgcgg tgatgtattg ggggttcacg atccctttgc
    18841 ccagagtctg agccaccgcc gttcccagaa accatgcgcc gatgagctcg aagagggcga
    18901 tcaagaaaat gaccttgcgg ggttccatag ctccggtgat caccggagtg acgaccatgt
    18961 tggcgcagtc gttgaacccg ttgctgaatt cgaagacgag gacgatggca atcagcagga
    19021 tcgccgtggt caagacgttg aattccaaaa aatcacgctc cgtaagcctg gcgtaatacc
    19081 tggaccgtgt gaagggtttt cactccggtg tgaaacccga tctgcagccg gcaggcttcg
    19141 caatcggaga tgacagtgcg ggctttcgat gaacggatgg cccggaacag gtcttccccc
    19201 acctccctgg caatggcata cttttcctgt ttgtagccat agcttccggc caatccacag
    19261 cacccttcgg gaagctcaat taccctcaat cctgggatca gggaaagaag ctccagagcc
    19321 ggcagcccaa tcttcaaagc cctcaagtgg caggggacat ggtagtagaa agtctcctgg
    19381 gtctcctgaa accgggtgtc tagtttaccc tcttcgtaca gctgcagaag aaactggcat
    19441 atttcaaaga catgatcctt gagcagcgaa gcgccgcgga ggctcaggag tgtttcatat
    19501 tcttctttaa gagtcattcc gcagcttggg gaagagaaga ccacttcggt tcccccctcg
    19561 atcgtttttc gcagggattc gacatttctt tctcccagcc gagaggcgag ttcgaaaaac
    19621 ccattactga taaggggaag gccacagcat tcctgccggg ggaggttaac ccggaacccg
    19681 ttctttccca aaacctcgac cacggccaat ccaagacccg gatcgatata attggtataa
    19741 cagccgtgga agtaggctat gggatccccc tttttctggg gatgggattg gagtctttgg
    19801 gcaaaggtcc tgcggcgata ggcaggcatg ggagtgcccc gggcgattcc cagtccccgg
    19861 tcgagaagcc accggaaaaa tccccatccg aacacccagt tagagatcgg ggtggcccag
    19921 cttcccagtt tctgcagaag gtaagcgtgg ccgaggagcc agtccctgaa agggggcttg
    19981 cgggtctttt tccaggccag gctggattta atgtgcaggt cgggaatggg tacgttctgc
    20041 gggcagaccc gctcgcagac cttgcagtag tcgcaatact ccagccactc caagggaatg
    20101 gacttcctct gaaggcggaa acgttccgca tccggtccgg ccagcttggg cccgggaaaa
    20161 agccccgtgg ccttcagcac tggacagtag gcgttacaga tggaacattt gatacactga
    20221 tcgatattgg gtttcatact tattttcgca caacagagac acagagaaca cggagaaagc
    20281 tttccacaaa attcacatca ctaaaatgga ttcatgagga tggattcaat ttctatgcct
    20341 tttgaatccc ttttaccttt atgccctctg tgtctctgtg gcgaatcctt ttcttgcgca
    20401 ggctgcggcg aattgaccag ccacataacc cgtagcgatg gccacgccgc ctccggattt
    20461 ctcggccatg gaatcgccat gggccaggat ccctccggca gcatagaggt tttgataaat
    20521 cacccggcct tctgagtcgg ccggctggag attctcattc acttctaccc cgaaagaatt
    20581 gaagggctgg ccgatggagg tcaggagttg ggcgctgaac cattccttcc gattctgcgg
    20641 gtattggacc ggaaggtcga agagagtctc gaaaatcctg ttccgttgcg catcgagccc
    20701 cccgcccaca aatttaccgg tggccaggac ataggcggat gcccggtaga ccgggctttt
    20761 ccttgcctct ccgagagcga tgccctgaat ctcccctttc tccaagatgg gcttcaatgc
    20821 cgaaagaccg atgatgacgc gcaccccttt ttcctggagg tgcgccctca tcaagttata
    20881 aaggcggaga cccgggatgg aaggaggggg cagggaaact tcaaagatat cggttccaag
    20941 tcttttttgg aggtcgcccc aggccgactg ggaggagaaa tacccaagga ccgcaggcaa
    21001 cccgagtttc tccccgcttt tcaacagtgg ttggacctcc cgtgcgactc gttccctgaa
    21061 ttcttcccca tcgaaagctc tggccaggtt caaactgttc agcgcctttc cctccaattt
    21121 cactcgggtc agggtttcag cccgaaaaga aggggcgatt ttgccccggg cccgccagag
    21181 attcaggttt tcagacgcca ggcaggcgga aaaatccttg agcccttcga atcccagaag
    21241 aaggaccctt cctggtaagg atagaacccc gttcttcatg gtttccggaa ctaggcaggt
    21301 gggatgataa gttcccagag cagtagggat caacacgctg gcattgaact cccccgcgta
    21361 agggaggccg ttcgaaaggg tcaagttctg gaatcgagcg agcgctgaga gaatgctttc
    21421 attccccagc cttgagtaag ggtgcgcggg gttcgccctt ctaagttcac ccaagttttc
    21481 gaggggagag gaggctggag cggcggagga ttcaggaaaa tatcccagcc cgtcgatgca
    21541 accgctcgtc aaaggaagcg agcccatgcc cttggccaag atcaaaaccc gggagccctt
    21601 ctcctgggcc gcttcagcgg ccatgagtcc ggccagcccc gctccaatga cgataacatc
    21661 atagaacata aagggtcctg ggtttcagga ggtcaaggat tcgatggcaa gggcaagaaa
    21721 aaagcttttc gcttggtccc ttgaatcctc tttcttataa gtggtctaaa ttgaacagct
    21781 ccgagtacag agcctggatc aactgttctt ccttgagctg ggggccccat aggatcggac
    21841 gggttccttt ccagcgctct tcgaggaatt ttttgagaac gtcaagagat gtgccctggg
    21901 ttattttccc ggtttccgcc aagagcgcgg tcagccgtgg agagcagaat cctccctggc
    21961 aggggcccat cccggcccgg gttcgctcct gcacttcttt caaagttggg aatgggagtt
    22021 tcttaatttc ttccaggatc tctccgcggg tgaccaattc gcactcgcag aggatttccg
    22081 aagaagaccc ggagccggaa atccggctca gtctggatcc cagatcatga aaagagtatt
    22141 ctctgggaaa ggggagaggt ttttccgctg tgacgcaggg cgcgttcttt cccatctcct
    22201 tgcatatggc atctacggtt cgctcggcca tcagacgata ggtagtaagc ttgcccccca
    22261 caatggagaa gagccctttt accccgaccg cgttcccatg gttgagtagg agaaaatctc
    22321 ggctgatggt tcttgtatcc ccttcggctt cccgggctcc gatgagcggt cttaccccgg
    22381 cgtaggctcg gatggcccga gtgacgccgg cgacagggat taccatttcc ccctcggaga
    22441 gaagaaggtc tacttcctcc tcgcttgctg acgggtcctc aatctccacc gtcgccaggg
    22501 aggtcgttcc gagaatcagc gccggcccgt ggggaacaaa gatatctcca ttcgaggggg
    22561 gtcggcagcg gttgatcaca gtgttgacca gacgctggtt aaggacaatg agggttccct
    22621 tggaaaagac gacctccaga cgggccccgg ccatgcgggc aatttttccg gcccataccc
    22681 cggcggcatt cacgataaaa cggcagccga tctgaaattt ctctcccgtc cggctgtctt
    22741 cggctgtcaa tcctcggacg gcgccttttt cggtttgaat agccacaact tcccgataag
    22801 gaaaggcctt cgccccgagt tgtaaggatt cttcaacatt ggcgcgaatc agcctgaagg
    22861 gatcgatggc cccgtccgga acccggatgg ccctaacaat gcggggggaa agagcgggtt
    22921 ctttctgcag agcatctgcc gcgggcaatt cttccgcggg gattccgatt tcttcgcagg
    22981 ccttgaggaa tcggtccgga taatccggcg ggtcatgggg tgtggaaagg aaaaaacccc
    23041 ctgtgtcctc gacacagggg gtggcgatct ttttgagaat ataattttcg cggatacatt
    23101 cccgaccgga ttcagggtct ttgaccgcgt accggccacc actgtgtagg aggccgtgat
    23161 ttcggcccga ggttccgctg gtgaattctt ttttctccag gagaatcgta tcgatgcccc
    23221 gcagggcaag gtcgcgaaaa acacctgccc ccgtggctcc cccgccaatg acgaccactt
    23281 cagttttcat aagaagaaca gccttcagcc atcagctatt cgcgatctgc taagaccaag
    23341 gcaagattta ttcggctgaa agctgacggc tgacagctta tttcaatatt tccgctacgc
    23401 tctcccagac ataatccggt tgaatatcgg atttcttcag gctaccccga gcggtcacgc
    23461 cggtaagaac cagagcggcg gccatacctg ccttttttcc catcgccatg tcggtttcca
    23521 gacgatcgcc cacgaggatg caatcctgag gacgaaggcc catgtaatcc atggttaccc
    23581 ggatcatgac gtccgagggt tttcccacga tcgtctcgac cttctttccc gtagttccct
    23641 ccacggcccc gatcatagcc gcgcagtccg gaatttcacc gccttccaca gggcaggtcc
    23701 gatccggatt ggtggccacg aaacgggccc cttttttaat ggcctggaga gcaatattga
    23761 gcttgcgata atcgaaggtt cgatcgaagg aagcgatcac atattgaatt tctcccgcct
    23821 tttcgctaac ccggaagccg gctcgaacca tttcttcaat gagcggggtc tcgccaatca
    23881 cgtaaatagt cgctcccggc gcttccctgg aaagccagag ggccatgacc aaagaggagt
    23941 ttatgacttc ctcgggtcga gtaggaattc ccagacgggt caacttgtcc gcatattctc
    24001 cccgagtctg gaggggcttg ttggaaaggt aaacgatctt cttggaaagg gactttaatc
    24061 tcttgatcgt ttccctggcc ccagggatca gcttttcccc caagtagatc gttccatcca
    24121 gatcaaagag gtaacccaaa aaatgcaggg catccatgaa gactctgttt ctccataatt
    24181 tcaccgacct atatttaaat ttatttgcca ggatgaggtc ttgtcaatga taaaataccg
    24241 cgggatcatt agaaaaatct gaatccactg gcccggtgca tttctattga caatcccttt
    24301 accctaagct atttttacag cgattggagg ttcccggtag tttgccggaa tcaattattt
    24361 cacctcttca aggaggacaa ggaaaatgaa gcgaaaattg atattaggaa tctttttgtc
    24421 tgtctttttg ctcctgcctt tttcatttgc acaggcccag tcgattgaaa atgaactcta
    24481 cctgatcacc ccggtttcca aagatgtcca tgatcctgcc ctcaatgcct ttgccgccta
    24541 tgccaagaaa aaatggaaca tggatgtgaa gaccagcgct attcccaagg ggactccggt
    24601 ggcctacgga cagatcctgg aatggaaggg caaaccccag gcagatattt tctggggagg
    24661 cgaagggact ctcttcgatg ccctggccgt ggaggggctt ctggagccgg taaagattcc
    24721 caagcagatg tgggatgaga ttcccgccac catcggaaag cctgtggggt tgcccctgaa
    24781 agacccgaag aggttctggg tgggaaccac cctggagcct tacggtctga tctaccagcc
    24841 caaattgttg aaccggatgg gggtggaaat caaagactgg gatgacctcc tgaatcccaa
    24901 actcaaaggg cagatcgccc agtgtactcc cgatcggtcg agctccagcc acgccagcta
    24961 tgaagtgatg cttcaaacct acggctggga gaagggctgg gagtggttga aaaaactggc
    25021 ttccaacacc ggcatattta ccgctcggtc ccgcgacgtt cccaatgttg tggccaaggg
    25081 ggaatttgct gtcgggtacg cggttcccag ctacatggct tttgccgagg tcttggccgg
    25141 gtatgacgtc atgtatgtgt accccaaaaa tgcctatgtg actcccgaac ccatggcggt
    25201 tctcaagggg gcgccccatc ccaaagcggc ccatgctttc atcgaattct tgcttaccga
    25261 agaggggcaa aaactcttcg ccgaactcgg cctctatccc atcactccga agttcaaagt
    25321 ccagggaccg ccgggctcca aacaggaaat gaccgtgaaa ttcaccggtg ggatgaggtc
    25381 tttcttcgac attccggtgg ggaacgtgta tgacgaaaag attgccggcc ccaagaaaag
    25441 aatcgaagag gtgaactcct acttccgcaa ggagattgcg gagaagcaca aagacattgt
    25501 aagaagcaaa tgaacttcgg actcagccct ttcttggtcc cggaaaaact cctcggggga
    25561 tgaccttgag gagtttttcc gttggttcag ggacgaaccg attggttgtt ctatgaatat
    25621 tctgctcaaa gacctagtaa aaaggtttgg aaacctggag gcggtgagcc atatttctct
    25681 ggagattcag gatggagaac tcttcaccct cctgggaccc tccggatgcg ggaagaccac
    25741 ccttctgcgg ctgattggag gatttcacag accggatggg ggagagattt atttcggaga
    25801 cagacctgtg gcccctattc ccccttacga gcggaatatc ggcatggtct tccaaaacta
    25861 tgctctctgg ccgcacatga ccatctttga caatgtcgcc tacggcctca aactgaggaa
    25921 gttttccagg gaggaaatct cttcaaaagt ctcccgcgct ctccagctgg taaacctgtc
    25981 cggactggaa aaacgttacc ccgggcagct ttccggagga caacagcagc gcgttgcctt
    26041 ggccagagcc ctggttctaa acccggacgt tctcctcctg gatgaacccc tgagcaacct
    26101 ggacgccaag atccgcatcc aggtgcgggc cgagattcgc aaactgcaga aggaattggg
    26161 gatcaccacg atctatgtca cccacgacca ggaagaagcg ctgaccctgt cggaccggat
    26221 cgcagtgatt gatcgtggaa aaatgcagca gctgggctca ccccgcgcgc tttacgccaa
    26281 gccggaaaac cccttcgtgg ccgattttat cgggatcaat aacttgatac cgggggaggt
    26341 tcgggaggtc ctggatgggg agaagatgaa ggttcaaacc gaggtggggt tgatcttctg
    26401 caccatcgag cgtcctttta aaccggggga ccgttgcatg gtaaccatcc ggccggagac
    26461 ggcttccatc atccaggagg aagttcggga aaaagatacg aatgttttgc caggcacggt
    26521 gaattttgcc tcctacatag ggaatacgat ccgctatgac ctggaaatgc agaacggcaa
    26581 aatcttcaag gtggatattc agaatccctg gtatcaccag attttggccc agggggaaaa
    26641 agtatatatt tccattcccc agcagatcac tctgggaatt cccatttcct gaagagagag
    26701 tttttccttg agtccccaaa ccgaaacatc gatcgcgatc cctcaagccc ggcccttcgc
    26761 cgagatgaaa ctcgggggtc tgctcgggta tttggccatc tgggcctttt tcggcgtttt
    26821 tctcatctat cccctgattc ggttgttcta tgacgccttc accaccacgg aaggcgtcct
    26881 taccttgaat aatttcttcg atttttttac cgactcctat tatctcaaat cgctagtcaa
    26941 ctctctttgg ctcgggggag ccacggtcgt aaccacctcg atcttgggta ttaccatcgc
    27001 ctatctcctt ctccggtacg aattcccggg ccgcaggctt ttctcctatc tcaccatcat
    27061 tcccatgatc atgcccccaa tggtgggggt gatggggttt gtcttcatca tgggacgggc
    27121 cgggaccctg aacataatcc tcatggatta tctcgggttc gagaagccca ttaatttcat
    27181 gtacggcgtc catggggtcc tcctggtgga aactcttcac ctctttccac tcatgacgct
    27241 ctccatcgtc gatgccctgg ggaagatcga cctctctctg gacgaggcgg cggagagtgt
    27301 gggctccagg gggctgcgga aattctggga catcaccttt cctttgacga ccccaggata
    27361 tgtgtccggc gcccttctgg tcttcatctg gacctttgcc gacttcgcca ctcccctggt
    27421 cgtgggagtg gatgacctcc tcgcttccca ggcctacctg aatatagtcc agtttgtgga
    27481 ccggcggctt ttcaagatgg ggatcgtgat ctcggccatc atggtccttc tggcgattct
    27541 cttcctcatc atcgccaaga aatacgtggc catcaaagac tacagctccc tctcctactc
    27601 caaggtcgaa aggagaaaaa tttcagcgtg gggaaaggtc ctggtcatcg tctttctagt
    27661 agcggtgctg gtcttttcct tcattcccta cctggggatc gctctggatt cctttggcaa
    27721 aggatgggcc ctgaccccga tcccggtgaa gtacaccctc cagtacttcg agcgagtggc
    27781 gatcgaaacc cccaagttca ttgtcaatag cctgcttttt tccggcatct ccgtattgat
    27841 ttgcatcatc gtgggagtcc ccgtggcctg ggtgatggcc cggaccaagc ttcccggaaa
    27901 ggatatgctg gactccctta ccaccttgat cctggcgtta cccggcacgg gcatcgggat
    27961 cgcctacatg cgggctttcc gcgatgccct gcctttcatg gacaccgccc tgatcggaat
    28021 gtgggtcgtg atccccatcg tcctgggagt gcggcgtctt ccctacacgg tgcggggaac
    28081 ctttgcttcc ctcctcctgg tccataaatc catcgaggaa gccgccgagt ccgtgggggc
    28141 gacaaaatcc aaaaccttca gggatgtggt agctccgctg atctggaaag gaatcatggt
    28201 gggggccctc tactccttta tccttgccct gcaggaagcg tcggccactc tcctcctggt
    28261 gattcccggc agggagatga tgaccgtggg gatcttcaac ttctacatcg gcgggtcggt
    28321 gaatgaggcg gcagccctag gatttatcct gatccttctg ggagcgttct gtctttggct
    28381 gatcaatcga ctcgccggct cgcgcatggg cggagtattt gggtaaagtg gaaatcggat
    28441 tgcggaatca gtatttgaac gcagatgaac gcagattttc aggattttag cgcctaaaat
    28501 agaacgaaaa attttttctg cgttcatcaa tgtaagttta tagcccatat ttatggaaaa
    28561 taagaaactc tggggaaaga ggtttgagaa aaaaccttcg gcgctggctg aagccttcac
    28621 ttcaggcagg gacgtgcgag gaatttctcc ggccgatcag cggctgatcc cttatgatct
    28681 ctggggcagc cgagctcatg tggtcatgct ggtcaggcag aggattatcc cccggcggga
    28741 cgcgaggctg ctggtaaccg gtttacgaga aatagaaaaa tattggcaaa ggggaagctt
    28801 taatctggat ccttcactgg aagacgtgca caccaatgtg gagggatggt tgattgagaa
    28861 gtttggaatc gaggtagggg ggaaactgca caccgcccgg agccggaacg atcaggtcgc
    28921 cctggacatg cgcctctacc taagggactg caatctgggc tttgtatccg gcctttcctc
    28981 cctgattcaa acactccttc gccgggcgaa ggagcatcgc ggggtcatca cccctggata
    29041 tacccaccac caatcggctc aggtaaccac cctggggcat atatggcttt cttttgctgg
    29101 agccttctcc agggatgtcc agcgttttca ggactggtac aaccggttta atgagaaccc
    29161 tctgggttcc atgaccggat acggtaccac ctttcccatt gatcgctttc ttacctcgaa
    29221 gcttctcggg tttgacggtt tgtgcgagaa ttccctggat ccgatccaga atcgctggga
    29281 agcggaggct gaactcggtt ttgcagtctc tgccatgatg aaccacctga gctctctcgc
    29341 ccagactctg attctcttca gtaccgccga gttcggtttc gtcaagctgg atgatgccta
    29401 cagtacggga agctccatga tgccccaaaa gcgtaatccc gatacgttgg aggtgatgaa
    29461 agccaaggct tccgcagctc agggtttcct ggcgtctctc ctttccattg gcaaagcgct
    29521 cttcttggga tacaaccgag acactcaatg gacgaagtac ttaatcttgg accttgtgga
    29581 tgaatgcctt tccgccgccc cgctcatggg ggaaataatc tcttcgctca aaataagccg
    29641 gaaagaaatg gaggcccgaa gccaagcagg ctttattgcc gcccctgagc tggtggagca
    29701 gatggtccaa gagtggaaaa tacccttccg gcaggccaaa ggggcggtcg aaaaggcgat
    29761 ccgctatgcc gagaaggaag gattcaaagt gctccctctt cctttgctgc agaaggcgtc
    29821 gcaggaggaa ggattaaaga tcaaaatgac taagtctttt gcccgaagat ctcaaaaggg
    29881 atatgacttc atttcgaaac gagtcgctgt cggaggccct tctcctcggt ccctggatcg
    29941 caatatttct accttgaaaa ggacccttca ggaattccac cgctggctca agcagaaagt
    30001 caccgagcaa tcccgtgcta aaatgaagct agcccggatg gaacaggccc ttaatttata
    30061 aacctcaatt ctttggaaag cgaaggggct ggccttctcc tgaatctcct accgcggaat
    30121 cagggggtgg gttccgttgg agatggccac cgaaatccgc caatcctgga agcctgcgcg
    30181 gttcatttcg tccagtagga tcgggagaag aatattcatc cgggccaccc gggtttcatc
    30241 gctgacgatg atggatatgg accctttggt ggggatgatc taagaagagg ggcccgggcg
    30301 aatgcccggg ccggatcgat aggtgggaga ttcttgccca cctttttatt accagagccc
    30361 tagggccttc caccagggga agccgacccc aaaccagatc acgaggttga tgatggagac
    30421 caggagtccc aatttccacc aagtgccgag gtccacatac ccggcgttgt aatagatggg
    30481 agaagggcct gttccgtaat gggtaattcc ggctcccagg gccgaagcca ttccaaagag
    30541 gaacaccgtc agttggggag gagcctgagc ggccatggca atggccagga agggggggaa
    30601 catggcggtt accttggcgg tgacgctggc aaaaccgtaa tggctgtaaa agtaaaccag
    30661 caccagaacg agcagagcgg gaagccaggc caaaccggtc acgctgccgg cgaccgaagt
    30721 ggcgacccat ttcatgaatc cgagttgact caagcccgtg gccatcatga ccaggccgcc
    30781 aaaccagatc aaggcgtccc aggcgccctt ttcgcccagg acatcttccc agcttatgac
    30841 tccggtcagg agcatgaccc ccaccccgat gagggccacc accgtagcgt cgagcttgtt
    30901 caattccgaa gttacccaaa gggccaggat catgaagaag accaccagca gaatcttttc
    30961 gctccgtttc atgggaccca ttttctgcag ttcggcatgg gccatctgag aagcctgggg
    31021 cgtttccttg atctccggcg gatagatttt ataaagaata taaggtgtaa ggatcaggca
    31081 caagagcccg ggaaggaccc cagcaagggc ccacattccc caggaaatgg ttatattggc
    31141 tgtctttttg gccagctcaa tgagaagcgg gctcgccgct tgcgaggtca tgaacatggc
    31201 ggaggtcacg atgaccgctt ggtactccat catcatcagg taagagccga tcttccgggc
    31261 agtagctccc ggctcggacc cgaaggtgct ggtcagacct ctaacaatgg gataaatgat
    31321 tccccctgcc cgggcggtat tggaaggagt cgcgggggag agaacgagat cgctggccgc
    31381 gatggcatag gccagtccca gggtcttccg tccgaaagcc cggatgaaga gataagaaat
    31441 tctccttccc aggccggtct tgataaagcc gcgggtcaga agaaatgcgg ctacgatcag
    31501 ccagacggtg ttattggcga atcctgaaag gatctgtccc actttgaggg tttccgtcac
    31561 gccggtcaac atgacgccga tgatcaccac cgcccccata ggcagaggag tcaagatcag
    31621 ccccacgatg gtggcgacaa aaatggccag aagatgcatg gcctccttct tgacccccgc
    31681 cggcgcgggc caataccaga tgatgatccc gatggcaatg gctatgagcc cgcggatgat
    31741 ctgtgatgtt ccgcttcgtt cctttccgtt catttgattc tcccttggat caattgatac
    31801 aactgatcgg tttactcaaa cttcttcaaa gctgatgtgt ccccattcta ggacgctttt
    31861 ccgtattccg ctttgacctt ctcccggtca accgtcccct cctgtgtttt gggaagggcg
    31921 gggacgaagt ccacgtattt cggcttttta taacgggcaa tcttggaggc tacgaaatcg
    31981 atcagcccct gctgggtgag aacgcttccg gtcttcaaca cacagaccgc cttgaccgct
    32041 tctccccatt ccttgtctcg caccccgaag accgcgacat cagcaacctc cgggtgttcc
    32101 aaaattgctt tttccacctc ggcggggtag acattctccc cgccgggctt aatcaattct
    32161 ttttctgcct ttcgttttac ataccagagg tatccgtttt cgtcgaggcg tccgatatcc
    32221 cccgtgtggt gccagccctg gcggaaggtg taacgggtgt cttcttccag cttccagtac
    32281 cctaaaaaaa ccagggggcc gcgtacgcaa atctctcccg gagttcccac cgggacttcg
    32341 cggtcatgat catccaccaa tttgatcttc gccagaggtc cctcttttcc cgccgaaccc
    32401 ggtcgctcgg tgtaggcgca caccgtgacc atccctgtgg tttccgattg gccaaagccg
    32461 atccagaatt cgacatttgc tttcttcttc agtctttcga tattcgatgg atgatcgatt
    32521 ccccccacat gcttcaggga ggagatatca taattcttcc cctgcaactt ctccaggagc
    32581 atccccagca tgggaggaaa ttcgccaaga gtcgtgattt tttctttttc gatgagttgc
    32641 agggcaagtt cgggatcaaa tttggtcatc aggacattct ttcccccggc gtgcatgacc
    32701 gcgaaggaaa gggccagatc ggcaatatga aagagaggca gcatatgaag gtaactatcc
    32761 acctcgcgca agcccatagt cgccatgtgc tgtagattcc cggcgatgat gttcccgtgg
    32821 gatagagttg cgccccgcgg ctttccggca actgccgcgg tgtgaataat taaataggga
    32881 tcgctcgctt gaatatcaac ttcggtcgcc tttccttctc ctttcatcag ggtgctaaag
    32941 gcttcaaatt ctgcctctgc ctctccaaag gaataacatt tttcaatgaa ccccatattg
    33001 ggcattaatc cacggattgt ggttttgaaa tccggtccca caaaaagaaa acggggccgg
    33061 ccgtcggaaa ggatgtattc cacctccggc gcagaaagcc gccagttaag ggggagcata
    33121 atagccccca gcctggcagc agcaccgtag aggaggacga attcatagca attctgggat
    33181 aaaattccaa tccggtctcc tttttttaat ccggcaatct ccagattata agctaatgag
    33241 ttgatattat tataaaaatc tatgaaagtt aggcgttttt ggtcgatgac ccatgctatt
    33301 cggcgggcaa agatgagcgc attgcgggca atcaaatcgt agatagtaaa gtcttgcaga
    33361 ctcatattcc ccctccattt aattttttgg ctccgctgtt gattcaggac tctatttgtc
    33421 agtatttctg ataaattccc agcttttaac cagtccgttt tcaaaataaa cgtaggattt
    33481 gggaaaaggg ggttccccgt atacccattg ttcgtgaatc ccgacgagag ttcgggtagt
    33541 attcacatga tccggctttc cccaggacag aacgacctgt tctcgggtga atccctggcg
    33601 gataaaaccg cgaagaacat cttcttgaat acgaatgggc catagggtct tggaaaggtc
    33661 ctttaggaga gttctcatct catctttatt cttagtgctt cgtcggatcc cattattttc
    33721 cgccacccat ccaagaattt cttccctaaa ggtagagttg gtcccatagg tgacttcgta
    33781 aaaggtttgt ttctgagggg ttaaagctat tctgctggtg aagtggacat cctctgacct
    33841 gatctttaca gctttagcat tcgctatatt ggcaaagtca cccaggtaga cccagacggg
    33901 caacttattc tttacccaaa aggacctctc ttcgggggac cagccatatt ttttcccctt
    33961 gtctgaaatg acccgttctc ccaccctgac ttgatgccaa tagtttccgt cttcatcctt
    34021 ttgggattgg attacttcaa ataggtcata tttttcgccc ctggatagga ccttggattg
    34081 ttctcttggc tcttcgtgaa ttgcggcttc cttcataacc agaaagactc cgctctcggt
    34141 tatttttggt aaaggagcag aagcgggggg ttgagggggg agaagaaggc cctcttttcc
    34201 tcccgattta taaaatttga tcctttgatc ctcctcggaa accttgcgag cttcctccac
    34261 cacctttctt tgccgttcct cctcagccct tcgccgctct tgttccaggc gacgttggct
    34321 ttctgcttgc agccttttct tctcctcgat ctcagctatg attttcccct tttccgctag
    34381 ggttttttgg atttcccttt ccagctgaat tctctcccgg accaggcgaa ttttcaactc
    34441 ctcagttcga gactgctctt cccagacctt tctttcgata caagcccaga ctcgaacccg
    34501 gtcatcaggg gaaagccttt tctctatggg agtgtccaga atcggctgca tgctcaccag
    34561 aacaggatta ttgggcggtt cctcgataaa attgaatact cgattctcac agagatagat
    34621 caaacggaag gaatatcctt cgtcatgaaa aaggaaataa tcgctttttc cttcccgagc
    34681 ataaatttct tgaactatct ttggaggttc agcgggatct ctgagcagaa ggctggtctt
    34741 gggcttggat gagcaggaaa tcgaaacgaa aacacctaaa agaatcagga aacagagaaa
    34801 aaactttcct agccccgccg atctgaagat ttcaatacct tcttttttgg ttttccggcc
    34861 catggcttgg ggcgggcggt aatccgcttg cccagctttt ggttgagaaa tcggaattct
    34921 tttttcataa ttgaatgaga aaagattagg cattaaaaag ctaaattagc agacaaaaat
    34981 acaaaagtca aacccctttc atttcttgcc ccctcacttt tcagcaagtt ggcttgagct
    35041 ctattgaatt tcaaacattg ccctacctgg aaggggggcg ttgggcggaa aaagccataa
    35101 ggcacggatc atttcttgga ccgggcaagt aagctgttct tctgccaaga acagggatat
    35161 tccgttgcag aggcgcccgg ccgggggcct gaaaggcccg cctcatcttc cggaaacacg
    35221 attggacctc cggttgtaat tctttttccg cttatggttt ttccgcgcaa cgcccttact
    35281 gcccccgggc caaaagcttg ctttttcgca gaaatgaaaa agacaaaagt gaaggggcaa
    35341 gcccttttaa ttcttttagc gattattgat cattcaccgg acgatcgttc attggattcc
    35401 tttcccgggg aaaggataag gtggttcgcc aaaacccatg attaaatatt tttttcctag
    35461 agttagaatt atctcacgag gcgagttgga tctgaacatg gtagaattta tcgaacatga
    35521 atattttttt gaatcttttt gatgaagata tttattttag ttctacttta tgaaaatata
    35581 ttttcataaa gtactactat ttgtgatatc atataaaaat ggttaagaga tctttttggg
    35641 ctaagctcct tgaaacagca tcggcacgta attcggtcat ttggctgaca ggagttccgg
    35701 gttcaggaaa gagttctctg tgccagggct ttggggatat tgagtatttt gactgcacta
    35761 ttccaaaagt tcggatggag atggaggatt ccgaaggatt tctcagaaag ttgctgggta
    35821 agacagtggt cctggatgaa attcaggagg tctccaatca tcaagaaatt ctcagcatag
    35881 ttgcaaacca atcctcccct cccaaaatca ttgccacgag ccctctaagt ctccattcaa
    35941 atccaaatct ttttgatact atagggaaaa aaataacgca ggtatggatc actcccatga
    36001 tgtcccagga cctcctagat ttcggccaaa caaacctggc tgatcggttt ctaagaggtg
    36061 gacttccatc ctttttcttg aatggcagga aaccagaacg cgaattccaa gaatgggtta
    36121 atctgttctg ggcgagagga cttcaggagc cgtttcgggt agagagacgg aattctttca
    36181 atagattcat agaactactc tttgcccaaa gtggcgaact ttttgaggct aagaggttca
    36241 ttaacccctg cggaatgagc catcctacgg taatgaaata ccttttggcc ttgcaatctt
    36301 cctggttggt ccagcttatt cggccattct ccacgagaca tgcctcggaa atcatttcta
    36361 tccccaaaac gtacgccttt gataccgggt tcttctgcta tttccgggga tggcaggaac
    36421 tgcgacaaga ggatttcgga attctctgga agcattgggt cttgaatgaa ctaaacagcc
    36481 atctccaaag tagggttatt cattattgga gggataaagg agggcacgaa gttgatttta
    36541 ttctcataac caaggagatg gggattacgg ccattgtgtg ccaatggaga gaaaaggatt
    36601 ttcagtcaaa aaatttgctt gccttccgac gtcggtatcc tgaagggcgt aactggatcg
    36661 tctgtggaga taatgttcag gctcatactc aaaccatggg taaaataaga ttagaattca
    36721 tgaccctaaa ggaaatgagc aaatggaccg ccccgctgtg ccagatagag gataacgcct
    36781 gaccgtccat aaaaattcga atatctgtga aaccatctca ttaacctgac taaaatattc
    36841 agcgtgtaat agaatctcac ttgagagatt tctattctga cccccccctt ttaagaaagg
    36901 ggtgaatcat aagaagacct caatgaaacc ccaagaaggt aataatttgt ttcaagaaac
    36961 ccacttcttc tccaaaggaa aaggcaaagt aagataaatt gatcccctta gcttttcggc
    37021 aactaagcat taaaactatt gaatttcaag cattgccagg ccacaagctt gctttttcgc
    37081 aaaaatgcaa aacacaaggg ggtaaaagaa gggttgacaa atattttttt caatgatact
    37141 taaaaagtcg ttttgcgaac gtcttaggaa tacaatcgat acatgtttca cgtgaaacat
    37201 acccgcttcc gaaggattta aattattctt ttgaatactt acccaaccaa ttacgatgtc
    37261 atcgttgtcg gagcaggcca cgccggaagt gaagcggcct tggcctgcgc ccgcatgggg
    37321 cattcaactc ttctcctaac catcactctg gaatcggtgg cgcgcatgtc ctgcaatccg
    37381 gccattgggg ggttggccaa gggacagctg gtaaaagaga tcgacgccct ggggggggag
    37441 atgggcaaga gcaccgacga gacgggaatt caattccggc ggttgaatac caagaaagga
    37501 cctgcagtac ggtcttctag ggcccagtca gacatgcaac tctatcacct gcagatgaaa
    37561 agggtcctgg agcgccagag aaacctcttc ctccgccagg ccttggtaga cgagctcatc
    37621 attcaaaggt gtagagctga gggagtagtg accaatatcg gggaacgttt tctttccaag
    37681 gctgtcattc ttactacagg aacctttctc cgcggcctcg tccatatcgg tctaaaaaaa
    37741 tttccagctg ggaggctggg agacctaccc tcaaccgggc tatccgagtg tttgtcgggg
    37801 cttggttttt ccttgggacg tctgaagacc ggcacctgcc ccaggttgga tgggagaact
    37861 atagattttt ccggccttga ggagcaatgg ggagatcatc cgccgaagcc cttttccttc
    37921 agccatcgga cagtcccgct tcgtcaagtt ccttgccata taacctatac aaattcaagg
    37981 acccatgatg ccatccgctc aggtctaaac cgttctcctc tctattcagg ggtcatcaaa
    38041 gggacaggag ccagatactg tccctccatc gaagacaaaa tcgttcgttt tgccgaaaag
    38101 gaccagcatc aaatcttctt ggaacccatg ggtctggaca ccctggaagt ctaccccaat
    38161 gggctggcta ccagtcttcc catggatatt caggtccaaa tgcttcgctc cattccaggc
    38221 ctgcagaggg tggagattat gcgccccgga tacgcaattg aatatgacta tgccgatccg
    38281 ctccaattga aaccatccct ggagaccaag ctcgtcgaaa accttttttt tgcagggcag
    38341 atcaatggaa cttctggata tgaagaagcg ggagctcagg gcctgatggc agggattaat
    38401 gcggtcttga aaatcagggg cgaggaacct ttcattttgc gaagagatga ggcctatatc
    38461 ggggttctcc tcgatgacct ggtaaccaag gggaccaaag agccctaccg tatgttcacc
    38521 tctcgcgccg aataccggtt attattaagg gaggataacg ccgacctgcg gttgagggaa
    38581 aagggaagga ccctcggcct ggtaagtgat gaggattacc aagaattcat cctgaaacga
    38641 gaaaagattc aaagggcaat agaacggatt ggaagaacca taatccaccc atccgccggg
    38701 gagaatgatc tcttgagaga gcttgggagt tctcccatta aaaacccaac ctccctttct
    38761 gacctccttc gtaggccgga aatttctttc cgccaccttt ccgtttttgc cccggccttg
    38821 gcctccaccg aagagaatgt ggccgaagag gttgaaatcc agattaaata cgaaggctac
    38881 ctgcgaagac aaagtgaact agtggaaaag gtggtaaagc tcgagcagat gaaacttccc
    38941 cccacagtgg actacgcaag tattccagga ctttcaaccg aggttaaaga gaagctgaca
    39001 agggtgaaac ctctatccat cggacaggct gctcgcattt ctgggatcac tccagcagcg
    39061 ctttccattc tgatgattta tcttcataag gcatcaccaa aatgagtata gcatatttat
    39121 aacttattta tatttttata ttttattaaa taataaaata atcttagcac tgcagatctg
    39181 caccaaccgg taatccggta atattgatct cttgggggtc ttcttctaaa aaattttttc
    39241 tcataatggg gctcattatg gagcgaagcg caaagagcag aaaagagttg accgatcccc
    39301 ccaaccagaa ggagattttt attgcccttg gataaagtag agacccatca gcctcaaggg
    39361 aatctgagat ttacccatgt taaatagaaa tcgaactcct tttgaggaga aaatatctcc
    39421 ggtagctatt caggagccct ctcaactcct ccgtgaaggg gcttccaagt tgggagtttc
    39481 gttgactgag aaacacctta atgactttga gctttacctt aaggaactca accgatggcg
    39541 aaagaagata aacctggtaa gccgaaagga tgaccgggaa attgttctta aagatttctt
    39601 ggattctctc accttggtca aatatatttc cgggatggct ttcctattag atttagggtc
    39661 gggggctgga tttccggggt atccgttgaa gatcgttctc cctgacttga aagttttttt
    39721 cctggaagcg actcggaaaa aggtgttttt cttgaaggag gtccaacggg ctttgggcat
    39781 gggcggcgtt gaggtccggt ggaccgctgc gaagaaaggg atagaagact tatcctcccg
    39841 cttcgatttc gttgtttccc gggccttcgg gtctattcag gaattggctt tttcgggcca
    39901 ccgatttctc tgtaagggtg gaatgcttgt ggccatgaaa gggaagaaag gaagggagga
    39961 gttgaaggaa ggcctccctt ctttgataga aatgaaactg aagttggcat ttctggaatc
    40021 acttcaaatt cctttcttaa atcatgaacg gaccctcata gggctcatta agatatgaag
    40081 ttccacgtga aaacaaagcc tttctttttt ccttgaaatt tcttccgcga ttaggataag
    40141 aatttggact ggagaacgaa atccatggga agagtcatca gcgtcgccaa tcaaaaagga
    40201 ggagttggta agacgacgac atcggtcaat ctttctgcat cgctggccgt agcggagaaa
    40261 aagaccctgc tcatcgacat cgatcctcag ggcaacgcta cctccggatt gggaatcgga
    40321 aaagaaaaaa tttcggatcg gaatatctat caggctctga tcgatcaggc aaatgtgaag
    40381 gaattgattc tggatacgga acttgaatat ctaaaaatca tcccttcgaa catcgacctc
    40441 atcggggcgg agatcgagct cgtcgccttc ccggaccggg aagtccgttt gaaaagagcc
    40501 ctgaatgatg tgaggggaac cttcgattac ttgatcatcg actgtccccc ttccttgggc
    40561 ctaatcacgg tgaattcttt aaccgcttcg gattcggtcc tcatacccct ccaatgcgag
    40621 tactatccaa tggaaggtct ttcacatcta ttgaaaacta ttagattaat taaggataac
    40681 ctcaatacgg acctggccat tgaaggaatt ttgctgacca tggtggataa aaggaataac
    40741 ctttcccacc aagtttcaga ggaggtacgc aagcattttc ccaacctggt tttcaacgcc
    40801 gtaattcctc ggaatgtgcg cctgagcgaa tgccccagct ttggaaaacc aatcatctta
    40861 tacgacgcaa gttcccgagg ggctgaaagc tatttggaac tggccaggga gattatcgcc
    40921 cgcaatcaac aaggaagaga aagataaatt tttcttttga catttttctt tttttcagtt
    40981 taaagtgaat cgtcattcat ttttttattt aaagtaaatt cagcagcaaa gccatatcta
    41041 aggattggtg aatttgaccg cgagacaggc attgggaaaa gggttggggg ctttaatccc
    41101 agaaaaaggc atccccgagg cggatgggaa aaaagcccct cagatttgtg ggatcgaaga
    41161 aattcaaccc aaccccttcc aacccaggaa ggtattctcc gaggaacaac tccaagagct
    41221 gatcgattcc atccgggaaa aagggattct ccagcccctg ctcgtccggc gcaggggaaa
    41281 tgggttcgaa ctcatcgccg gagaaagaag gtggcgggcg gcccagaagg cgggcatgaa
    41341 agaagtcccc atcctggtta aagacgtctc ggattcagag atgttggagc tttccctggt
    41401 cgagaatatc cagcgcgagg acttgaatcc catcgaagag gcagaagcct ataaacgttt
    41461 gatggaacaa ttcgatctca cccaggagga aatttccaaa aaagtaggga aggatcggac
    41521 cacgattacc aacacggtcc gcctgctcaa actccccccg gaaatcaagc aaagcctagc
    41581 cgaaggcaag atcagtatgg gccacgcaag ggcctttctc agcctcgacg ggatcgagaa
    41641 gcagaagctt ttattgaaaa ggttgcttac ggggggtctg tctgtccgcc agaccgaaag
    41701 cctggttaaa agatttcgga cccgcaattc tcctgtttcc cgaaagacca acccggaatg
    41761 gagcggcctg atggaagaac tccagcgaat cctggggacg aaggttcgga ttgtgggaaa
    41821 acggaaacga ggaaagattg agatcgagtt ttattccctg gatgagctgg atcgaatcct
    41881 ggagcttttg cgaaaatagg aaagtttaca aggaggaatc acctgaagaa aaaaaaggaa
    41941 agttccagcg aagagatcaa cgcccttctc ggcgggggaa cggaattcga agggaaatta
    42001 tcctttgaag gagcggtccg gatcgacgga aaatttaccg gcgaaattcg cggggaggga
    42061 atgatcatca tcggcgaaaa agggaatttt cgcggggaaa tcgaggctgg ggtggtcatg
    42121 gtcagggggg aagcccaggg aaccatccgg gccaagaatc gcattgaagc ctatatcccg
    42181 gcgaaaattc acgcagacct ccattccccc gtgctagtat tcggcgaagg ggtgattttt
    42241 gagggaacct cgcacatggg agcagaggcc aaagagggag acaatcaccc aattccaggc
    42301 accaaataag aaaaaggaaa tttaaaacca ccaaaacaaa aaggagcatt cgggttttgg
    42361 atttgatcga gagcttggca tttagaaatt tttaaggagg gttccctctt ccaagttagg
    42421 aaacccatgg agatatggga tgatcgatct gaattttacg gtaatctttc agctggccat
    42481 cgttctgatc cttatggttg ccttaagcaa cctggtcttc aaaccttttc tccgcgtcgt
    42541 tgaggaaagg aaagactgga ttgagggggc cgagaaaaag gcccgtgcgc tccagcagcg
    42601 cagcgaagaa ttaatggagc ggtatcaaga ctccatgagc gcggcccagg cccagggggc
    42661 ttcgatccgg gaggagatcc gcaaggaggc actctccaag gaggcgcaga ttctgcagaa
    42721 agccatggaa gagggcaatc aatttctgga ggagatgaaa ggtaagatcc gggaggaaag
    42781 ccagaaggcc aaggccggtt tgagacttca ggcccagacc ctgtctcggg aaatggccga
    42841 aaaaatgctg gggaggagta ttcagtgaga aaattcaacc gcttgctggg gttgagccta
    42901 tgggtgcagt ttcttggggt tggaaacctc tgggccgccg ggggtggggg aggcttctcc
    42961 ggtgaagtga tttggcagat catcgccttt cttcttctag gtttctttct cgctaaaatg
    43021 ctgaagaagc cactgggggc cttcctggcg aaaaggaggg aggaaatccg cagttccctg
    43081 gatcaagcgt ccaaaaagga gagtgaggcc cagaggcttt tgggagattg ggaaaagaag
    43141 ctagaagccc tgagccagga ggtcagggac ctccaggaga cgatccgtaa agaaggagaa
    43201 gaagaaagga aaaaaatcat cgagcgagcc gaggaagaag gggaacgggt ccggaaacag
    43261 gcccagatca tcgccgagca cgaggtgaaa aaggcccgga tggctttgaa gaaggaaatg
    43321 gtggaccttt cgctggagct gacggaaaaa ctcttgaagg aaaaaattca acctcaggac
    43381 caggagaggt tagtccatga atatatcggt aaaatgaggg agctccggtg atcagcttaa
    43441 ccatcgcgcg aaaatacgcc aaggccctgc tggacgtcgg acggagagag aggagtcacg
    43501 aacttctggg gaaggatttg gataaactgt gggggcttct gcaggaaaat aaggaactga
    43561 ggacagtcct cttcagccct ttctacccgg ccccccggag gaaggcgatc gcccgaagcg
    43621 tcggtgaatc tttagcccta tctaaatcgg tccttgattt tctcaacctg ctcatcgagc
    43681 gggaccggat ggaccatttc ccctccatcg tcaagtccta cgaagaactc tgcgatgagg
    43741 tttcgaatcg tctgcgggct tccctggcct cgccggagat acttccttcc cagcttgtgg
    43801 gtgagatcca gacccaactg gaatccacaa ccgggaaaga agtgatcctt tccaccctgg
    43861 aagacccttc gctgatcggg ggcgtggtaa ccaaaatcgg aaacatcatt tatgacggga
    43921 gcctcaaaac ccagcttttg aaggccaaag agaacctata caaggagtga gccaagatgg
    43981 ccgtacagat taaagccgac gagattaccg agatcatccg tagtcaaatt cagggatttg
    44041 accggaagct ggatgtggcc gagaccggga tcattctttc tgtgggggat gggattgccc
    44101 gggtctacgg cctggaaggg gtcatggccg gggaacttct ggaatttccc catggggttt
    44161 tcggaatggc cctcaacctg gaacaggaca acgtgggagc cgttctcttc ggcgaggaca
    44221 agctcatcaa agaaggggac atggtcaaga gaaccaaacg gatttcccag gttcccgtgg
    44281 gagatgagct catcggacgg gtggtgaact ccctgggcca gcccatcgac gaaggcccgc
    44341 cggttgaggc caagcatttc gtgaacatcg agcagaaggc tccgggaatc gtggtccgcc
    44401 agccggtgaa agagccgctc caaaccggcc tcaaggccgt tgattccatg attcccatcg
    44461 ggcggggtca acgggaattg atcatcggag accggggcac cgggaaaacg gcgctggcgg
    44521 tggacgcgat catcaaccag aaggggtccg gggtctactg cttctacgtg gccatcggac
    44581 agaaacggtc caccgtggcc cgggtggtcg atgtcctaaa aaagtacggg gccatggagt
    44641 acaccacggt cgtagcggcc accgcttccg acccggcgcc catgcagtac atcgccccct
    44701 acgccggctg cgccatgggg gagtacttcc gggattcggg aaaacatgcc ctctgtatct
    44761 acgacgacct gtccaagcac gcccaggcct accggcagat ctctctcctc ctgcgccgtc
    44821 cgcccgggcg ggaagcctat cccggggaca tcttctatct ccattcccgg ctcctggaga
    44881 gggcggccaa gatgcgcgat gacctgggag gcgggtccct gaccgctctg ccgatcatcg
    44941 agacgcaggc gggcgacgtt tcggcctaca ttcccaccaa cgtcatttcc atcactgacg
    45001 ggcaaatcta cctggagtcg gagctctttt actccggggt ccggccggcc atcaacgtgg
    45061 gactatcggt ctcccgggtg gggggcaacg cccaaatcaa agcgatgaaa caggtcgccg
    45121 gaagcctccg cctggacctg gcccaatacc gggagatggc tgctttcgcc cagttcggaa
    45181 gcgacttgga caaagccacc caggcccaac tggcccgggg gtcccggctg gtggaaattc
    45241 tcaagcagcc ccagtaccgc cctattcccg tggaaaagca gatcctgatc atttttaccg
    45301 ggacgaacgg gttcctggac ggctatccgg aaagcgcctt ggcacgttat gaaaaggatc
    45361 tttttgagtt cgtggataaa cagtactcgg cggttctcac agacatccgc cagaagcgga
    45421 cgattgacgc cgaactggag aaaaaggtga aagacctcct ggaggagttt aaaggaaagt
    45481 tcaaggcgga gtaaccagcc atgccgaaag ccagccttcg cgatatccgc aaacgcattg
    45541 ccagcgttcg atcgacccgg cagatcacca aagccatgaa gatggtagcg gcggccaaac
    45601 tgcggcgggc ccaggaaaac attttggcaa cccgtcctta cgccgccaag atgctcgagg
    45661 tcctccgcag cctggcggcc cggacttccc cggatgccca tcctttatta taccggaggg
    45721 aacccagacg gatcgaactt gtggtcatca cctcggaccg cggcctctgc ggggccttca
    45781 acatgaacct catccagaag gccgagcgct tcgtggaaga ggagaagacg ggcgctgaga
    45841 gcttgaccct ttcttttatc ggccggaagg gccgggatta ttttcgcaaa aaaaaggtaa
    45901 ccatccgcca ggagtatatc aaccttttcg gaaaggtgga ctatcctctg gcagcccgca
    45961 tcgggcaaga cctggggaag tcctacgtgg cggaccgggt ggatgccatc tatctcctct
    46021 atagcgaatt caagtcggcc atccagcagc gggtggttct ggagaaggtc ctgccgctgg
    46081 cccccggcct cctgaaagaa gcggaaaagg ggaaagaaat atccccggcg accgccggta
    46141 ccgcggctgc cgtggattac atctatgagc cttcggaagt ggagatcctt gggaaaatcc
    46201 ttcccatgta cgtggaagtg caggtgtacc gggccctgct ggaatccgtg gcctccgagt
    46261 tcggggcccg gatgacggcc atggaaaacg ccaccaataa cgccggggag atgatcgaca
    46321 agctcaccct ggtttacaac aaggcccgcc aggcggcgat caccaaggag ctgatcgaga
    46381 tcgtcagcgg ggccgaggcg ctgaaataga aaagaagcgg tcagcgctca gccaaaagaa
    46441 gaggagccga tttttcgctg atagcttgtg aaggaggaaa cccgtgatcc aggttggaaa
    46501 gattgtccaa gtcatcggac cggtagtaga cgtggaattc agcggcggag aacttccgcc
    46561 catcttcggg gcgctcaaga tcaccaaccc ccgcattgat gcccgggagg agaacctcat
    46621 cgtcgaggta gcccaacacc tgggagacga agtggtccga tgcgtagcca tggatacgag
    46681 cgacggattg gtgcggggga tgaaagccac ctactacggg gagcagatca ccctgcccgt
    46741 cggccgggaa accctgggcc gaatcctgaa cgtggtagga gaacctgtgg atgagatggg
    46801 gccggtgacc gccaagatgc gctacccgat ccaccggccg gcccccgagt ttgccgaaca
    46861 atccactcag atggagcctt ttgagaccgg aatcaaagtg gtggaccttt tggcccctta
    46921 cctgaaaggc ggaaagatcg ggctcttcgg aggagccgga gtgggaaaga cagtggtcat
    46981 catggagctc attcacaatg tggccatgga acacggcggc ttctccgtct ttgccggcgt
    47041 cggggaacgc acccgcgagg gaaacgacct ctggctggag atgaagcagt ccaaggtgct
    47101 ggaaaaagcc gctttgatct acggccagat gaccgagccc cccggggccc gggcccgggt
    47161 gggcctttcg gctttgacgg cggccgagta ttttcgcgat gaagaagggc aggacgttct
    47221 tctcttcatc gacaacattt tccgcttcac ccaggcgggt tcggaagtgt ccgcccttct
    47281 ggggcggatg ccctcggcgg tcggctacca gcccacgctg gccacggaga tgggggagct
    47341 ccaggagaga atcacttcca ccaaacgggg ctccatcacc tcggtgcagg ccatctacgt
    47401 tccggccgac gacctgaccg atccggcccc ggcgacaacc tttgcccacc tggacgccac
    47461 caccgttctt tcacgggctc tggtcgagtt gggcatctat ccggccgtgg acccactgga
    47521 ttcaacctcc cgcatcctgg atcccctggt gattggagaa gaacattacg cggtggcgcg
    47581 tcgggttcag ttcatcctcc agaaatacaa ggacctccag gacatcatcg ccatcctggg
    47641 gatggacgag ctctcggagg aggacaagat catcgtatcc cgggcccgga agatccagcg
    47701 tttcttgtcc cagcccttct ttgtggccca ggagttcacc gggactcccg gggtttacgt
    47761 gaagattaaa gacacggtcc gggggttcaa ggaactggtg gaaggaaagt gggaccacct
    47821 tccggagcag gccttttaca tggtagggcc cattgaagat gccgtcgcca aggccaagcg
    47881 cctgggagcg atggtctgag gaagcggccg tggataagac tttcctgctc gagattgtca
    47941 ccccatacgg tttaattgtc agctccaaag tggaggaggt atacatcccg gggagtcagg
    48001 gagatatgga tgttctcccg ggacatgccc ctctcatcac ttctctaaga gtgggagagc
    48061 tgcactataa gcaggagaaa gaggtccact acctggccat caaccgggga tttgccgagg
    48121 tcactccggc ccggaccacg atcctggtgg atacagcgga gcctgctgtt gagatcgatg
    48181 tgactcgagc ccgggcggcc caagcccggg ctgaggagag gctgaaaaac ctcactccca
    48241 gagacccgag ctacccccag gagcaggaag ccctggagcg ggcaagggtg agactgcggg
    48301 tcgccgagaa ggctccccgg gaataggact gtcctcaagg tttcaggaaa agacttcccg
    48361 cgggcgttcg cccgacctgg gggttcactt ccgactgatt cagccttctg ctcggtatgg
    48421 ggaattggtt caagattggg gtagccctgg gagggggagg ggccagaggg ctagcccacc
    48481 tgggggtcct gtgcgccctg gagcgggaga ggatttcctt cgacctcctg acgggcacct
    48541 ccatgggagc cttggtcggc ggaatgtatg cgct
//
LOCUS       DASPRR010000338         4922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1371156, whole genome shotgun sequence.
ACCESSION   DASPRR010000338 DASPRR010000000
VERSION     DASPRR010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371156"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..949
                     /locus_tag="VEL68_12760"
     CDS             158..949
                     /locus_tag="VEL68_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXZ36885.1"
                     /translation="MGEKIWGLLTVFFISALLFFSPTLPAAEIGKGVKITYLGHSAFK
                     LVSPQGAVIYIDPYLKSNPQTPADQKEVGTADLILPTHGHADHLGDTVAIAQKTNASV
                     VAMAELATYLTKKGVKNVVRMNKGGSYTAKGVRITMVSAQHSSAVTEEGEVIYTGEPA
                     GFIIRFENGFTVYHAGDTAVMSDMKIFGDIYKPNLALLPIGSHFTMDPQEAAYACQLL
                     QPQFAIPMHYKTFPVLTGTPEDFTQAMKEQPGVRVIVMNPGQTIE"
     gene            1047..2888
                     /locus_tag="VEL68_12765"
     CDS             1047..2888
                     /locus_tag="VEL68_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ36886.1"
                     /translation="MNGYTGRILHIDLTQQKFKIQNFDEAFAKKFLGGNGFAAKILYD
                     GLKKGIDPFSPENLVVLAVGPVTDSPLPSTSRAYVATKSPLNGLFFDSTFGGRFAITQ
                     KRTGFEAIVISGKAASPVYLVIDETGAQFKSAGPLWGKLTKETFDAIQAVEGEPADVV
                     AIGPAGEKKVRYACLVHYWRGREGVSGRGGVGAVLGAKNLKAVVVKGSKRTQVADSQA
                     IQELIASRKEPMEKGTAGLKNLGTPVLVNMINTIGGLGVRNLQEEQSPHAPEIGGERL
                     KELFFDRNDTCFGCPIACGKTLRLPPEQGGLRWKMPEYETLFSLGSMADNWDAASMLK
                     LNRLCDQYGLDTISMGVTISFALECFEKGYLTSGDTGGRNLHFGDSALLLELVEDTGK
                     RQGFGDVLAEGSFRLAERLGKESANLLYCFKKVEVAGHSARALKGMSLGYATATRGGS
                     HHDARPTLQYAGEFDRTKAQIAPAFAFRTQNFTAMNDSLTQCRFASERGFGGVINENY
                     SKMINGVTGWNFTLEDIEKIGERIYNLERAFNCREGVTRMDDALPYRASRQPISDGPS
                     KGMYTPGDEFDGMLEEYYRLRGWDSNGIPTREKFMALGLEGEARDLK"
     gene            3135..4064
                     /locus_tag="VEL68_12770"
     CDS             3135..4064
                     /locus_tag="VEL68_12770"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ36887.1"
                     /translation="MSEGQKKQSKLMTAAEAVRRFVSDRALVGMGGQSIGRSSMCIYH
                     EMIRQRKKDLVLVGCNLSIPMDMMVGLGLVRRTECGTGNLERFGTTHCWRKAVEQGKL
                     VNEDYSHLAMVTRFLGGEMGLPFMAIQSLLGSDILAKKSASTGKKYEIVDNPWNPGEK
                     VVLLPSLNPDVSIIHAQKADETGNLIIEGFLAHEPEMIRASRAVIVSCEEIVPSDEIR
                     QYPERTTIPYVYVSAVVHQPWGAHPTSVYRCYAHDSEHMREYQKAAREGGAAFEAYME
                     KYIFSCANFYEYLEKVGGLRKYNRLRQEMVEML"
     gene            4153..>4922
                     /locus_tag="VEL68_12775"
     CDS             4153..>4922
                     /locus_tag="VEL68_12775"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004970028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ36888.1"
                     /translation="MSSEYTLNELMIIEAARHIRDGETVFVGTGMPMVAAMFAQKTHA
                     KNMCFVVETGPISPEALPVPTSVSDPKAMHRAAKLGTLREVLGCLLQRGMVDVGFLGG
                     AQIDHYGNINSTVIGDYQRPKVRFPGSGGANDIASLAKRILVIAPHEKRRFPERCAYV
                     TSPGYLDGPEARKKAGLKSLYPDLTVVTDLAVMSVDKNDGRLKVNKLMPGVTFEKVQE
                     STEFKVEAASSMAQVPVPAPEELRILRDEVDPGREYLG"
BASE COUNT         1157 a         1347 c         1437 g          981 t
ORIGIN      
        1 ggaggcggga atccgggctt tccgggagtg ttatagaacc actgaaccga aagaagggat
       61 gaaggccttt ttggagaaac gtaaaccaag gtactaaata tcgttcattg atcatcaacc
      121 attggccatt gatcaatgat cagaggaggg ctagaccatg ggggaaaaga tatggggcct
      181 tttgactgtt tttttcattt ctgctctgct tttcttctcc cctacccttc ctgccgcgga
      241 gatcggcaag ggagtgaaaa tcacctattt ggggcactcg gccttcaagt tagtctctcc
      301 ccagggggct gtgatctata tcgatcccta cctgaagagt aacccccaaa ctcccgctga
      361 tcagaaagaa gtaggtacgg cggacttgat tttacccacc cacgggcacg cagaccatct
      421 gggcgacaca gtggccatcg cccagaagac caatgcctcg gtggtggcca tggccgagtt
      481 ggccacctac cttacgaaaa aaggggtcaa gaatgtggtt cggatgaata aaggcgggag
      541 ctacaccgcc aagggggtcc ggatcaccat ggtcagcgcc cagcatagct ccgcggtcac
      601 ggaggaaggc gaggtgattt ataccggcga gcccgccgga tttatcatcc gtttcgaaaa
      661 cgggtttacc gtttaccatg ccggagatac ggcagtcatg tccgacatga aaatcttcgg
      721 tgatatttac aagcccaacc tagccctcct tcccatcggc agccacttta ccatggaccc
      781 gcaggaagcg gcctatgcct gccagctgct acagccccag ttcgccatcc ccatgcacta
      841 taagaccttt ccagtcttga ccggcacgcc ggaggatttc acccaggcga tgaaagagca
      901 gccgggggtc agggtcatcg tgatgaaccc cgggcagacc attgaataaa gacaggtcat
      961 aggcatatgg accgacgcac gagggacccg aaaacaatac tgaaactcga aaatggatat
     1021 tttcatcagg ggtctggagg gggaatatga atggttatac gggaaggatt ctgcacatcg
     1081 atctgacgca acagaaattc aaaatccaga actttgatga agccttcgcc aaaaaattcc
     1141 tcggcgggaa tgggttcgcc gccaaaatcc tgtacgatgg gcttaaaaag ggaattgatc
     1201 ccttttcccc ggaaaacttg gtggtccttg ccgtgggccc tgtcacggat tctcccctgc
     1261 ccagcaccag ccgagcatac gtggccacca agtcccccct gaacggtctt ttcttcgact
     1321 ccaccttcgg agggcggttc gccatcaccc agaaacggac cggttttgag gcgattgtca
     1381 tctcggggaa agccgcctct cccgtctacc tagtcatcga cgaaaccggc gcccagttca
     1441 agtctgccgg ccccctgtgg ggaaaactga cgaaagagac cttcgatgcc atccaggctg
     1501 tcgaaggtga gccggccgac gtggtggcca tcggcccggc gggagagaaa aaagtccgct
     1561 atgcctgcct tgttcattac tggcgcggcc gggaaggagt ctccggaagg ggcggtgtcg
     1621 gcgcggttct gggtgccaag aacctgaagg cggtggtggt caaagggtcg aaaaggaccc
     1681 aggtggccga ctctcaggcc atccaggagc tgatcgcctc ccggaaggag ccgatggaaa
     1741 aaggaacggc gggccttaag aacctcggaa cgcctgtatt ggtcaacatg atcaacacca
     1801 tcggcggatt gggagtccgc aatctccagg aggagcagag cccgcacgcc ccggagatcg
     1861 ggggggagag gctcaaagaa ctcttcttcg atcggaacga cacctgcttt ggatgcccca
     1921 ttgcctgcgg aaagaccctc cgcctgcctc ccgagcaggg gggccttcgg tggaagatgc
     1981 ccgaatacga aacccttttc tcacttgggt cgatggccga taactgggat gccgcctcca
     2041 tgctcaagct caacaggctc tgcgatcagt atgggctgga caccatcagc atgggagtga
     2101 cgatttcctt cgccctggag tgctttgaaa agggatacct gacctcagga gatacagggg
     2161 ggcggaatct ccattttggg gattcggccc tgcttctgga acttgtggag gacacaggga
     2221 aaaggcaggg gttcggcgat gtgctggccg aagggtcctt ccgcctggcc gaacgcctgg
     2281 gaaaagaatc agccaacctc ctctattgtt tcaaaaaggt ggaagtagcc gggcattcgg
     2341 cccgcgccct caagggaatg tccctggggt atgccacggc cacccggggg gggagccatc
     2401 acgacgcccg cccgaccctc cagtacgccg gggagttcga ccggaccaaa gcccagatcg
     2461 ccccggcctt cgctttccgg acgcagaatt ttaccgccat gaatgattca ctgacccagt
     2521 gccgcttcgc cagcgagcgc ggcttcggtg gggtgattaa tgaaaattat tcgaaaatga
     2581 tcaatggggt caccggctgg aattttacgc tggaggatat agaaaaaatc ggtgaacgca
     2641 tttacaacct ggaacgggcc ttcaactgcc gcgaaggagt gacccggatg gatgacgcct
     2701 tgccctatcg ggcctcccgc cagcctattt cggacggccc atccaaaggg atgtacaccc
     2761 ctggcgatga gttcgacggg atgctggagg aatattatcg cctgcggggc tgggattcca
     2821 acggaatccc gacccgggag aaatttatgg ccttggggct cgagggggag gcaagggacc
     2881 tgaagtaaat gcggaaggcg gaatgttgaa tgcgggatat cagatgcacg gctaggaaat
     2941 gcggggtgcg cccttctcct cgtccccgcg tccttttcct tttttgtccg aattccacat
     3001 tttgcactcg aagggggagt tgaccctgaa gattgcataa tgtataaatt atacatgaac
     3061 tcgttgccct cccggttcct ggaaaggacg gcgggagacc gaggttctat tcttttcacc
     3121 ccgagggaag aattttgagc gaaggtcaaa aaaaacagag caaactcatg accgcggccg
     3181 aagcggtaag gcgatttgta tccgaccggg cattggtggg gatgggcggc cagtccatcg
     3241 ggcgatcgag catgtgcatt taccacgaga tgatccgcca gaggaaaaag gacttggtcc
     3301 tggtgggctg caacctctcc atccccatgg atatgatggt gggattgggt cttgtccgaa
     3361 ggaccgagtg cgggactgga aacttggaac ggtttgggac cacccactgc tggaggaaag
     3421 cggtcgagca gggaaagctg gtgaacgaag actacagtca cctggccatg gtcacccgct
     3481 ttttgggcgg ggaaatgggc ctccccttca tggccatcca gagcctcctg ggctcagaca
     3541 tcctggccaa gaaatccgcc tccacgggga agaaatacga aatcgtggac aatccctgga
     3601 atcccggaga gaaggtggtt ctccttcctt ccttgaaccc ggatgtttcc atcattcacg
     3661 cgcagaaagc cgatgagacg ggaaacctga tcatcgaggg attcctggcc cacgagccag
     3721 agatgatccg ggcttccagg gcggttatcg tctcctgcga ggaaatcgtg ccttccgatg
     3781 aaattcggca gtacccggag aggaccacga ttccctacgt ttacgtgagc gcggtggtac
     3841 atcaaccctg gggagcccac cccacttcgg tttaccgctg ctatgcccat gacagcgagc
     3901 acatgcggga gtatcaaaag gcggcccgtg agggcggcgc agccttcgaa gcctacatgg
     3961 agaaatacat cttctcctgt gcgaattttt acgaatacct ggaaaaagtg ggcggtttgc
     4021 ggaaatacaa ccgccttcgc caggaaatgg tggaaatgct ttagtaccgt tcggagttca
     4081 aagtccagag ttcggagtaa aggatttaat attcttccca attttagttg atttctatca
     4141 agaggctgga agatgtcttc cgaatacacc ttaaacgaac tgatgatcat tgaagcagcc
     4201 cggcacatcc gggacggaga gacagtattc gtgggaacgg gaatgcccat ggtcgcagcc
     4261 atgttcgccc agaagaccca cgctaaaaac atgtgcttcg tcgtggaaac cggccccatc
     4321 tcccctgagg cccttcccgt ccccacctcg gtctccgacc ccaaggcgat gcaccgggcg
     4381 gccaagctgg ggaccctgcg ggaggtcctg ggctgccttc tccagcgggg aatggtggac
     4441 gtaggattcc tgggaggagc ccagatcgac cattatggaa acatcaacag caccgtgatc
     4501 ggggattacc agcggcccaa ggtgcgtttt cctggaagcg gaggggccaa tgacatcgct
     4561 tccctggcca aacggatcct ggtcatcgcc cctcatgaaa agagacggtt tcccgagcgc
     4621 tgcgcttacg tgaccagccc gggctacctg gacggcccgg aggctcggaa aaaagcgggc
     4681 ctgaaatccc tctaccccga cctcaccgtg gtcaccgacc tggcggtcat gtcagtggat
     4741 aaaaacgacg gacgactcaa ggtgaacaag ctcatgcccg gagtgacttt tgaaaaagtt
     4801 caggagagca cagaattcaa ggttgaggct gcatcttcca tggcccaggt gccggtcccc
     4861 gcgcccgagg agttacgcat cctccgggac gaagtcgatc cgggccgaga atacctgggg
     4921 aa
//
LOCUS       DASPRR010000339        23667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2176698, whole genome shotgun sequence.
ACCESSION   DASPRR010000339 DASPRR010000000
VERSION     DASPRR010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 23667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23667)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23667
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176698"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1347
                     /locus_tag="VEL68_12780"
     CDS             295..1347
                     /locus_tag="VEL68_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HXZ36889.1"
                     /translation="MTSVWLVRAEDLREYSAAVLQAVGIPLANIKVISTALLDAELHG
                     LDTHGTVRLPQYVTLIKQGSINPRPEIKVVNESANHALVDNGYGLGFLGSVKAMNLCI
                     VKAKRTGIAMAGVCNSSHFGAAGYYARMAAERDLIGFATSNSYPVMAPPGSITRTHGN
                     NPFAFAIPSGKRPPIVLDMATSVVSQGRIKKYVEERRRIPLGWANDREGKPTDDPEAA
                     FFMLPLGEGGYKGYGLAMVIDALAGVLTGSLFARNFVSGPGSRGCGHFFMALDPQLFL
                     PLDDFKKRMDEMVGQMKDAKLPEGSTEPIYYPGERGFARKEKRLKEGIPLLGSTVKLL
                     DRLSDDLGLSRLKRIG"
     gene            complement(1618..1694)
                     /locus_tag="VEL68_12785"
     tRNA            complement(1618..1694)
                     /locus_tag="VEL68_12785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1658..1660),aa:Arg,seq:tcg)
     gene            complement(1846..1922)
                     /locus_tag="VEL68_12790"
     tRNA            complement(1846..1922)
                     /locus_tag="VEL68_12790"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1886..1888),aa:His,seq:gtg)
     gene            complement(1988..2064)
                     /locus_tag="VEL68_12795"
     tRNA            complement(1988..2064)
                     /locus_tag="VEL68_12795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2028..2030),aa:Arg,seq:tct)
     gene            2451..2789
                     /locus_tag="VEL68_12800"
     CDS             2451..2789
                     /locus_tag="VEL68_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HXZ36890.1"
                     /translation="MTCTFCQIIARELPAEILFEDKDLIVFKDINPEAPIHLLIVPKT
                     HFDSLNSLDEKTAQIGAQMFLAAKKMAESQGIQSGYKVFINCGRDAGQVIPHLHMHLM
                     GGWGRRPVRR"
     gene            complement(2856..4274)
                     /locus_tag="VEL68_12805"
     CDS             complement(2856..4274)
                     /locus_tag="VEL68_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXZ36891.1"
                     /translation="MISPNPENWTVTSLSKALRKREISPVEITRAYLERIQSNDPKIN
                     SFITLLPREALWAARQAEKERGKGKVQGPLHGIPFAAKDLFFTKGVRTTCGSKILKNF
                     IPAYNATVIERLQAAGAILLGKLNMHEFAYGTTSVNPHFGAVRNPWDRERVPGGSSGG
                     SAAALACSFVPLTLGTDTGGSIRIPSALCGTAGLKPTFGRVSKYGVYPLCWSLDHPGP
                     MTRCVADLAIAMNVLAGPDPLDSSTSPVAVPDYTRGLSTRLKGVHIGVPDTYYFERLD
                     PEVRARVLEAIRVFKALGAAVKNISIPLLPEASIAAFILLLAEGAATLEKWHRTRPMD
                     LGDDVRSRINLGAAIMATQYLKAQAVRRKIRETFSRVFQKVDVIVTPQLPITAPKIGQ
                     GTISWGRRTEAVPSALTRFTRIYNLVGIPSLSIPCGFSKAGLPVGLQVAGKPFDEATV
                     LKVGHAYESNTAWKDCHPRLDG"
     gene            complement(4271..4465)
                     /locus_tag="VEL68_12810"
     CDS             complement(4271..4465)
                     /locus_tag="VEL68_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36892.1"
                     /translation="MRKRAVKRTPGLPVRFSLSLSNKKEREIYLASIQQLDEQSDRLG
                     IFDPLDTEIAILYSPMEQKE"
     gene            complement(4771..5131)
                     /gene="ssrA"
                     /locus_tag="VEL68_12815"
     tmRNA           complement(4771..5131)
                     /gene="ssrA"
                     /locus_tag="VEL68_12815"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5196..5657)
                     /gene="smpB"
                     /locus_tag="VEL68_12820"
     CDS             complement(5196..5657)
                     /gene="smpB"
                     /locus_tag="VEL68_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXZ36893.1"
                     /translation="MGEKLLCQNKKAHHDYFIEETYEAGIALVGTEVKSLRLGKGNLK
                     DSYVQIEEGEAFLHNTHISPYPYGHQFNHDPERVRKLLLHKREIRRLMGKTQERGFTL
                     VPLKVYLKNGKIKLQLGLAKGKSLYDKRETLKKRSADREIEKVMKDAKGRR"
     gene            5831..6106
                     /locus_tag="VEL68_12825"
     CDS             5831..6106
                     /locus_tag="VEL68_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXZ36894.1"
                     /translation="METVLSRVFMIKNKFGLHARPSASFVQTASRFRSEIKVEKDGLV
                     ADGKSILDLMMLAAAQGTQLTIKARGADAQEALKALGDLIEGRFGED"
     gene            6108..7904
                     /gene="ptsP"
                     /locus_tag="VEL68_12830"
     CDS             6108..7904
                     /gene="ptsP"
                     /locus_tag="VEL68_12830"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXZ36895.1"
                     /translation="MTREPKKTQVIKGIGVTPQIIMGKAYLVDRGKIEAPARVLPENQ
                     VPWEIERFRDAVEESKKQLRSAKEKLLHEDAVGPTYILDAHLLLLEDKNVIQSTRETI
                     EQSWINSEWALKINLDRLRKIFDSIDDDYLKSRKTDLDYIEQRILRNLTGRGTEALSQ
                     IKEEVIVVAHDLSPADTAQMAKDKITAFVTDVGGTTSHTAIMARSLEIPAVVGSETAT
                     HSVNTGDTLIVDGITGVVVVNPSPEIIQEYMDRNRTRQRIERELFLFRDLPAETLDGF
                     RVNVLANIELVEEIPAVLDHGAEGIGLYRTEYLYLNRKELPTEEDHFQAYKTVVKAIA
                     PHPAVIRTLDIGGDKFISHLELAEEMNPAMGLRAIRFSLKEPEIFKVQLRAMLRASVY
                     GKVKILLPMISGVREIREVKKTLNEVRLSLTAERIPYDPKVEIGIMMEVPSAATISDI
                     LAKEVDFFSIGTNDLIQYTLAIDRVNEYVTHLYEPLHPAILRLVRQVVKAAHDAGIRV
                     AMCGEMAGDPLYVPVLLGLELDDLSMNVLSIFRVKKILRAYTLRECKELVEASLGLST
                     PEEIEALVRASLHRKFPEEFRELFNSKLSGNS"
     gene            7927..9090
                     /gene="metK"
                     /locus_tag="VEL68_12835"
     CDS             7927..9090
                     /gene="metK"
                     /locus_tag="VEL68_12835"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXZ36896.1"
                     /translation="MGMTDFLFTSESVTEGHPDKVADQISDAVLDAIIAEDPRGRVAC
                     ETLVTTGLAMIAGEITTSCYVHIPDIVRETIKEIGYNDSAMGFDWETCAVVTSIDRQS
                     PDIAMGVNESPDHEQGAGDQGLMFGYACSETPEMMPLPILLAHRLTRRMAEVRKKKIL
                     NFLRPDGKSQVTIQYVNGKAIRVDTIVLAAQHIPEVKYAALQEGLIEEVVKRVVPAEL
                     RDQRTKYYLNATGRFVVGGPRGDCGLTGRKIIVDTYGGVGSHGGGCLSGKDPSKVDRS
                     GSYMARYVAKNIIAAGLAEKVEVQIAYAIGVAQPVSVMVDTGGTWKIPPDDIAKLVRE
                     VFDLRPSPIIRKLNLLRPIFKKTACYGHFGRNDEDFTWERTDMAETLRKAAKL"
     gene            9256..10512
                     /gene="ahcY"
                     /locus_tag="VEL68_12840"
     CDS             9256..10512
                     /gene="ahcY"
                     /locus_tag="VEL68_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXZ36897.1"
                     /translation="MDYDVKDIHLAEKGKMRVEWAMKSMPVLKLIQRRFQRQKPLKGV
                     RLAACLHVTTETAALMMTLQAGGAEARVCASNPLSTQDDVAAFLAKDLKVPAYAIKGE
                     DHKTYYQHIHSVLDLKPMLTMDDGADLVSTLHTERRELLPGLKGGTEETTTGVIRLRS
                     MADKGVLRYPIIAVNDANTKHFFDNRYGTGQSTIDGIIRSTNRLMAGSAFVVCGYGWC
                     GRGVAMRAKGMGANVIIVEVDPLRALEAVMDGYRVMPIGEAASVGDIFCTLTGDIQVI
                     RQEHFLKMKDGAILANSGHFNVEIDLEALERISTGRRTIRDFVEEFRLRNGKKVYVLG
                     EGRLINLAAAEGHPSSVMDMSFANQAMAAEFIVKSRGKLAKKVYGVPPEIDQEIARLK
                     LASMGIKIDTLTPRQKKYLNSWEMGT"
     gene            complement(10603..11682)
                     /gene="lptG"
                     /locus_tag="VEL68_12845"
     CDS             complement(10603..11682)
                     /gene="lptG"
                     /locus_tag="VEL68_12845"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HXZ36898.1"
                     /translation="MKVITRYLAREFAQYFSIGLGGFSAIYLIIEFFERINVFLFNKA
                     TWPLMGAYFLNKIPSILFQVSPAAVLLASMITLGLMARHNEVMAMKSGGVSLGFLVYP
                     VLGVVILVFGGMLGLSEFIMPWANQNARSISDFIIHKKNPVATFKQSQVWIHGRQSIY
                     NIQLYHPNRNVLEGLTIYRFGPDYDLIQRIDARSAQWKEGQWILSEASVTDFDRKGVP
                     ARNYYPELILSLPETPSDFQIAEKNPDEMNFRQLREYVQKIEQDGYDSRKYRTAMQGA
                     ISFPFTVVIMACLGIPLALRSERGAGFARGLGYSIFISFIYLVVYSFILELGKGGTFP
                     PFLAAWLGNFIFAMVGVYLLLSVRH"
     gene            complement(11679..12872)
                     /gene="lptF"
                     /locus_tag="VEL68_12850"
     CDS             complement(11679..12872)
                     /gene="lptF"
                     /locus_tag="VEL68_12850"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HXZ36899.1"
                     /translation="MKRTLYIYLLKETVPAFLLGLFGFTLVLLTGRILQLTELFVNKG
                     VPLGYILRLLYLLLPQFLSLTIPMATLLSVLLTFHRLSGDNEITALKASGISLYQMIP
                     PILIFAFITYAVTTLLSLYAVPLANEASRALLFEVASTRANAGVRERVFNDDFDGLVL
                     YIEQVKPKTLTWENVFISDSRNPAEVFTIFAREGEVLSDPQALAVTLRLKNGSIHKLG
                     TDPHAYQKVEFNTYDLRLYLKTGLQEKKTEEKHPADMTLGELIQSIHSLKAKAEDTRT
                     QRVKVHEKFSIPFACLVFGIIGVPLGLQSRAGRGSKSRGLSWSIGILLIYYLFISAGT
                     SLAERGTIFLEVGMWAPNLIFLVLGIHLLVKAANESPVLIFVWVQKGIEWLRLKRQHG
                     GGIPG"
     gene            12951..13991
                     /locus_tag="VEL68_12855"
     CDS             12951..13991
                     /locus_tag="VEL68_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXZ36900.1"
                     /translation="MSIRRMLEEREAKIFSPYAQLSSQSRGRAVPEAECSIRPAFQHD
                     RDRILHSKSFRRLKHKTQVFLVPGGDHYRTRLTHTLEVAQIARTMAKALSLNEDLTEA
                     IALGHDLGHTPFGHAGEETLNEICPGGFRHYLQSLRVVDHLERDGRGLNLTFEVRDGI
                     VKHSKGKGDLIPDVPEELPVTYEGRLVRVADIIAYINHDLDDAIRAGLIFPDSIPRDC
                     VEELGDSHGKRIDSMVRDILEQAMKGDSLTIGLSERKLEATLKLRDFLYDRVYDLPTV
                     HGDFVKSAKILRELYAYFLDHPQEFRMEAGEAFSHEPVERSITDFIAGMTDRYAFKLY
                     EKIFLPQPWMII"
     gene            14344..14973
                     /locus_tag="VEL68_12860"
     CDS             14344..14973
                     /locus_tag="VEL68_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36901.1"
                     /translation="MTDKKDEPIIELTEVVEEAPESSGGPWMENSHPPAPPPAQQIRE
                     PESPPVLPRENAQKMDEALKRIPDSPLKKFILSEDETTGILPPPAKAPEFPTLSPQPP
                     RISPEPAKPTPAPKVTFPEAPRPHPEPPPMPPEPVQAPSEPPKSPVMDVEAELRIIRE
                     AMLSRVERWVSQEGVQALERVAREIFPKIAEEVLRKEIEKIKAEAEEKE"
     gene            14970..17642
                     /locus_tag="VEL68_12865"
     CDS             14970..17642
                     /locus_tag="VEL68_12865"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXZ36902.1"
                     /translation="MKEEEAKEWSKIYDPHRVEDKWYTFWEKRNFFRGDENSKKTPFS
                     IVIPPPNVTGSLHMGHALNNTLQDVLSRYKRMDGFNVLWLPGTDHAGIATQNVVEKQL
                     AEEGTDRHQLGREAFIQRVWKWKEQFGGHIIKQLKKLGSSCDWSRERFTMDEGLSRAV
                     REVFVRLYQEGLIYRGDYIINWCPRCQTALSDLEVEHEDDSGHLYYIQYPVDGSQEAL
                     TVATTRPETMLGDTALAVNPEDERYKKYVGRRVILPLMNRKIPVIGDGYVALDFGTGV
                     LKVTPGHDPNDFEIGRRHNLAVVKVIDEAGNMSQEAGKYSGLGRFQARDEVVKDLKAL
                     HLLQKIEPFPHSVGHCYRCKTVIEPLVSKQWFVKVKPLAERAIAAVVKGETRIIPQAW
                     ESTYFEWMNNIRDWCISRQIWWGHRIPAWFCEKCNEVIVSIEPVTQCPACGSSAVRQE
                     TDVLDTWFSSALWPFSTMGWPKKTKDLEVFYPTSVLVTGFDILFFWVARMMMMGLKFM
                     EEVPFREVYIHALVRDAEGQKMSKSRGNVIDPLEVIEKFGTDSFRFTLAAFAAQGRDI
                     RLSEDRISGYRNFANKIWNASRFTLTNLQGFNPEKSPGKTELTLADKWILSRVNRVVA
                     EVREGLDSYKFNEAASAVYQFLWHEFCDWYIELIKPALYQDQDPGRKWLTQNVLARVL
                     DTSLRLLHPFMPFITEEIWQNLPGNNGSIMVTEFPRIQENEVNLREEAQMELIMKVIG
                     AIRNLRSEMEIAPGKKVEAILYFPKKEPLHFLEENRSYLENLGRTEKLGLQSGGQRPK
                     ASATAIVDEVEVFLPLKGLINLDEEEKRVQKELAKVFEDLSRARGKLQNQDFLSRARP
                     EAVEKEKEKARVLSEKEAKLKESLERVKGWRKET"
     gene            17745..18734
                     /gene="hemB"
                     /locus_tag="VEL68_12870"
     CDS             17745..18734
                     /gene="hemB"
                     /locus_tag="VEL68_12870"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXZ36903.1"
                     /translation="MYFPLYRPRRLRKSEALRRMVRETKLSPEDFIYPLFIVHGRKAR
                     HEIKSMPGIFQLSIDLAVREAEEVCKLNIPAVILFGIPERKDLKGTQAYAPRGIIQQA
                     IRAMKRELPDLVVITDVCLCEYTSHGHCGVVVDGEVHNDATLNLLARMALSHAEAGVD
                     MVAPSDMMDGRVKAIRDVLDRKGFEDIPILSYAAKHASVFYGPFREAAESTPQFGDRR
                     SYQMDPANAREAIREVRLDVQEGADIIMVKPALAYLDIIRKVREEIELPVGAYNVSGE
                     YSMIKAAAQRGWIDGERAMMEVLTAIKRAGADLILTYFAKEAARFLEKNRSAC"
     gene            19067..20389
                     /locus_tag="VEL68_12875"
     CDS             19067..20389
                     /locus_tag="VEL68_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36904.1"
                     /translation="MRERKKISRRAFLKTAAAGIGGITLGLKGIEASAQDKTPAKANP
                     ARKALGVDVIKLGGQGVISGAHADYGWQIQGGATLAIEEINAKGGVLGSKLELKFMDE
                     ELKPAVAVKNARYLATEWGAHLLFGVDSSGSAMAIGPILPELDRICMFDHASTHRLTE
                     ELVYEKGIKQIFRIAAPVYQEGNLAAMIFKEFPNIKRWAGIHCDYEYGYSSWALFKDT
                     LKKYRPDVEFVAIAWAPFWTMDFSSHISAVMAANPDGIHATPWAGEGVMLLRQALMLG
                     VFDKIQAWFQCMGASVDILEGISNEVAAGKFKDKLWATARYIHNYPDTPENKKFVEAF
                     RKRWNRFPNYSAENAYSTIYVFKAALEKSRSLKTADLVKAIEGMKIMTPAGERFFRPE
                     DHQAIYAVPGGRVVKDPNYPIPVLGDLKVFPAQAYYRNPPFEPLKMGK"
     gene            20449..21306
                     /locus_tag="VEL68_12880"
     CDS             20449..21306
                     /locus_tag="VEL68_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36905.1"
                     /translation="METLAFQGLVGLSVAMYLWLLAAGLTIVFGVLGVLNFAHGSLFM
                     LGAYFAFTFYGLWGVNFWLAIALSLVSVAVVGYLMERFFLRHIYNQDHAYQLLLTFGF
                     ILILDDAVKIVWGGVAKIPAMPSFFEGTVSVVGRPFPVYNLFIIGAGVFVAILVWLLL
                     EKTWWGKMVRASASDREMAGAIGINISTLFTGVFAFGAILAALGGALGTPVRVVAPGI
                     GAAMIIQAFIITVIGGLGNLKGAFVGALIVGTLSAYGILLFPVFELFLIFLIMAVVLM
                     IKPQGLFGS"
     gene            21310..22245
                     /locus_tag="VEL68_12885"
     CDS             21310..22245
                     /locus_tag="VEL68_12885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36906.1"
                     /translation="MKTRLAILLLIIFLAFLPAFAGKYLLYVTIHILLLSIFSLGFNL
                     LFGYTGLLSFGQAGFYAVGAYGCAKILLVYPSLLAGILGGVALAGVASLLLGFLCIRH
                     TRIYFSMLTLSFGMMIFSLAWKWRDVTGGDDGLVGIPRAPLEVPGVFKINMAAMESYY
                     YVVLGLSLLAIYLFLRIVRSPLGLTLQGIRDSETRAACVGVSVRKYRLLSFTIAGLYA
                     GLAGTLLPPLESTVTPPVAHWSYSAEPVLATLLGGIYSFSGPIVGSVLFYLLKDIIVR
                     FTEYWLICLGIIVVVLVMGFRGGVVSIFLKEGSRR"
     gene            22242..22994
                     /locus_tag="VEL68_12890"
     CDS             22242..22994
                     /locus_tag="VEL68_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36907.1"
                     /translation="MKEIIRTEGLAKYFGKMAAVHEVTLTLQEGLFTSVIGPNGAGKT
                     TLINLLSGYLLPDRGKVLFQGSEITRLGPDKRVKMGISRSFQVMNIFPRMTVFQNILL
                     PVLSRLNKTSSAFSSLLSQKEAVGEAERILVEVWLKDEKDFPAGELSHGDQRLLELGI
                     AVASNPQVCFLDEPSSGMNPIERIKVLELVKKLAAERKTTFIIVEHDMDIVFSLSDWI
                     VVMNRGEILAEGRPDEIRANREVREIYLGEEV"
     gene            22991..>23667
                     /locus_tag="VEL68_12895"
     CDS             22991..>23667
                     /locus_tag="VEL68_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36908.1"
                     /translation="MTAVLEVKEIITRYGQSQVLHGVSLEVGQGEVVGLLGRNGVGKT
                     TTFRSIMGLTPPRSGSIRLKGVELGGKKSYEIARLGIGYVPDDRRIFPDLTTEENLEM
                     ARRLAKKGEGPWTLARVYQTFPVLEKLKSSKGTTLSGGEQKMLAIGRGLMKNPEILLL
                     DEPSEGLAPLVVRNLTEIILQIQKSGVTLLLADQNIKFCRKVAGRGYIMEKGVIQYEG
                     AMDDIWQ"
BASE COUNT         5500 a         6385 c         6877 g         4905 t
ORIGIN      
        1 caaagccata cgggataaga gaaaaataat agggaggaca agcgaaagga agccggatag
       61 ttcctgggcc cagaaaggca ttgccaagaa tttcagggcc aaggaaaaag atgtcttgga
      121 gatgataaaa aggctggaag caatgattat gaaaatcgcc cacgcgaaat cataacgacc
      181 gttttcaggc tcagtaaaac ccaaggagag atgacggtga tagaaggaaa agagacgggg
      241 cttttgggcg ttatatgaag ggcggggaga gagcgaggcg aagaggcata atttatgact
      301 tccgtatggc tggtaagagc agaagacctg cgagaatact ctgcggcagt gcttcaggct
      361 gtgggtattc ctctcgctaa tattaaagtt atctccaccg ctcttttaga cgcggagctg
      421 cacggactgg acactcacgg tactgtaaga ctgccccaat atgtaaccct tataaaacaa
      481 ggcagtatca atccgcggcc tgagattaag gttgttaatg agtcggccaa tcatgcgctt
      541 gtggacaatg ggtacggcct cgggttcctg ggtagtgtga aggccatgaa cctctgtatc
      601 gtgaaagcga agcgaaccgg aatcgccatg gcaggggttt gcaactcatc ccatttcggg
      661 gcggcaggct attacgcccg catggcggcc gaacgagacc tgattggttt tgccacctcc
      721 aattcctatc cggttatggc acccccaggg agtattaccc ggacccatgg gaataatcca
      781 tttgcctttg ccataccctc agggaaaaga ccaccaatcg tactcgacat ggccaccagc
      841 gtggtctctc agggtaggat caaaaagtat gtggaagaaa ggcgtcggat ccctctcggc
      901 tgggcgaatg atcgagaggg gaagccgacc gatgatcctg aagcagcctt ttttatgttg
      961 cccttggggg aaggtggcta taaggggtat gggctggcaa tggtaatcga tgctctggcc
     1021 ggtgtgctca cagggtcgtt gtttgcccga aatttcgtct ctggccccgg ctcccgtggt
     1081 tgcgggcact tctttatggc tcttgaccct caattattcc tccctctcga tgatttcaag
     1141 aaaagaatgg atgaaatggt tgggcagatg aaagatgcta aactcccgga gggaagcaca
     1201 gagccgatct attaccccgg cgaaagaggc tttgcgagga aagaaaaacg gctcaaggaa
     1261 ggaattccgc ttctgggttc aaccgtaaag cttctcgatc gattaagcga cgatcttggc
     1321 ctgagcaggc tgaagaggat cggttagtcg attttttctc ctggatggaa atatggaagg
     1381 aaagggaggg gagattcaag agaaaaataa aaaaaagtgt cccaagacgg acttgagttg
     1441 acagtgtagg gaagaaggtg gtcagataat acctcctgga ttgttagccg acaatcaaaa
     1501 aaaggaggta aagatcatga ccacccaaga gaagcttatc ggaagaaagc agagtttgct
     1561 agggtaggat gaacaaaaaa atgagttaag caattttgcc taactcattg tatttattgg
     1621 cgcgcccggc ccggctcgaa cgggcgacct gcggattcga agtccgacgc tctatccaac
     1681 tgagctacgg gcgcaagaaa aaaccaatgg gacgcagatg aacgcagatt tccaggattt
     1741 caaaaactat caatattgga cacggatgga cacggacaaa tacgcatgaa agcaaaatag
     1801 aaaaagttga tacaccgcca agagggcgga gagcacacaa aaagatgggg tgagtgaagg
     1861 gattcgaacc ctcggccgct ggggccacaa cccagtgctc tatccagctg agctacactc
     1921 accacaaaaa aaaatcgcta ccgcttgagg accgtcaagc catcggtcgc ttttttttcg
     1981 cgtggcctgg cgcgcctgga gggactcgaa cccccggccc acagcttaga aggctgttgc
     2041 tctgtccggt tgagctacag gcgctccaaa aaatttctac cattttatcg gtgacttgtc
     2101 aacaaaaagg accgtcacgc cgaggaaccg gcttcaggcc gtctcacacc agacagtcct
     2161 gaaccctccc aacctgcaac ccttaatggg caagtcttcc ctttgcttct aggctatggt
     2221 aaaaaggaac ctaatgcaaa ggcaataaat taaatgacat cacccgctcc ctaaccctcc
     2281 ccccttggag ggtgcgtcgt aatccgtcat gcccgcgaaa gcgggcatcc aggaagttcg
     2341 aatgttgaat aaattcaata ctagattccc gcttccgcgg gaatgacagc tcttttcttt
     2401 cattacgaca tggtctcttg agggggaggg gagggtggga aagggtaaaa atgacctgta
     2461 cattctgcca aattatagcc cgagagttgc cggcggaaat cctttttgaa gataaagacc
     2521 tgatcgtttt taaggacatc aaccctgaag ctcccattca tctcctgatc gtccccaaaa
     2581 cgcattttga ttctctaaac tccctcgacg aaaaaaccgc ccaaatcggg gcccagatgt
     2641 tcctggccgc aaagaagatg gccgaatccc aaggaatcca atcggggtac aaggtcttca
     2701 tcaattgcgg aagagatgcg gggcaggtaa tcccacacct gcatatgcac ctgatgggcg
     2761 ggtgggggag aagaccggtc agaagataga gcaggcgcgc gtttcaagct cagcccaggc
     2821 agggccctta gagccaatcc gcaaattccc ctacattaac catcaaggcg tggatggcag
     2881 tccttccagg cggtattcga ctcgtaggca tgtcccactt taaggaccgt cgcttcgtca
     2941 aagggttttc ccgccacctg gagtccgacg ggaagtccgg ctttggaaaa accgcaggga
     3001 atggagaggc tgggaatgcc gaccagatta tagattcgag tgaaccgggt cagggctgag
     3061 ggtacggctt ccgttctcct tccccatgag atggtcccct ggccgatctt cggcgcagtg
     3121 atggggagct gaggggtgac gatcacgtcg actttctgga agacgcggga gaaagtttcc
     3181 cgaatcttcc ggcggaccgc ctgagccttc aggtattgag tggccatgat ggcggccccc
     3241 aggttgatcc gcgaccggac atcatcccca aggtccatgg gacgcgtccg gtgccatttc
     3301 tccagggtgg ctgccccctc tgccaggaga aggataaagg cggcgatcga ggcctccggc
     3361 aacaaaggga tagagatatt cttgaccgcc gctcccaatg ccttgaatac gcggatggcc
     3421 tccaacaccc tggcgcggac ttcggggtcc aggcgttcga agtagtaggt gtcagggact
     3481 ccgatatgga cccctttcaa tcgggtggac agacccctgg tgtaatcagg gaccgcaacg
     3541 ggagatgtac tggaatcgag cggatcgggg cctgcgagca cattcatggc gatggccaga
     3601 tcggctacgc atcgggtcat ggggccaggg tggtccagcg accagcagag ggggtacaca
     3661 ccgtacttgc tgacccgtcc gaaagtgggt ttcaagccgg cggtcccgca gagggccgaa
     3721 ggaatgcgaa tggaccctcc agtatctgtc ccaagggtga ggggtacgaa agagcaggcc
     3781 agggcggcag ccgaccctcc gctggaacct ccgggaaccc gctcccggtc ccaggggtta
     3841 cggactgccc cgaagtgggg gtttacggag gttgtgccgt aggcgaattc atgcatgttc
     3901 agttttccca ggaggatagc cccggcagcc tgaagccgct caatcaccgt tgcattgtaa
     3961 gcagggataa aattcttcag gattttggac ccgcaggtcg tccggacccc cttggtgaaa
     4021 aagaggtcct tggctgcgaa gggtatcccg tggaggggcc cctgaacctt ccctttccct
     4081 ctttcctttt ccgcctgccg ggcggcccac aaggcttccc gggggagaag ggtaatgaag
     4141 gagttgattt tcgggtcgtt cgattgaatc ctttccaaat aagcgcgggt gatctcgacg
     4201 ggagaaattt cccttttccg aagcgctttc gaaagagagg tgacagtcca gttttcagga
     4261 ttgggggaga tcattctttc tgctccatcg gcgaatagag aattgctatt tcggtatcca
     4321 ggggatcgaa aatccccagc cggtcgcttt gctcgtccaa ttgctgaata gaagcaaggt
     4381 aaatttccct ctctttctta ttcgagagag acaaggaaaa tcggaccggc aggcccgggg
     4441 ttcttttgac cgctcgtttt ctcatttttg gttctcctca attttctgag ccccctgcgt
     4501 accctgggga gcacttcagt gaatgttttg aatgtcgggg ctcggtcagc atgcgcagag
     4561 aaacctccct catgcatact gctgggaacc ccctcggaaa actaagctca ggagtcgggc
     4621 tcagctgggc caaggacgcc cgagagcgct tgaacaaggg agaatcttat tcaagtgtct
     4681 tttcgacacc ccttttgggc atttgcaccg gaatgctgat gggcgcgaaa aatttggatg
     4741 gatagagata atcccagttc cgaaattcgt tggtggaggc ggcgggagtc gaacccgcgt
     4801 ccggaaacct tcagtaagga gcatctacgt gcgtagccgt tgatttgaat ctcgcctctc
     4861 cggacttcca gcggcagaat tcctggagaa gccagcttat gtgttttcgc cctccgccta
     4921 taagcttcag cggtaagcta tcctgtagtg tcgacgcccc ttttcagcct tacaggagaa
     4981 ggctggagga acggccactg tcaattaagc agtggcgtaa gcgtagttgc tgtttccgct
     5041 tatcgtttcc cgaccttttt aacgagaccc agtcggaacc tcggcacgca gccctcgcct
     5101 caatgatccc cgtcgagacc gtggcgcccc ccccaaaaaa caggtaagag gtttgaggtt
     5161 agagggaaaa gccagacccg aattttcaaa gagcgtcagc ggcggccctt ggcatccttc
     5221 atgacctttt cgatttcccg gtcggcggaa cgctttttca gggtttcccg cttgtcgtaa
     5281 agactctttc ctttggccaa acccaattgc agcttgattt taccattctt tagatacact
     5341 ttcaagggaa ccagggtgaa accgcgctcc tgcgtttttc ccatgaggcg acggatctcc
     5401 cgcttatgca ggaggagctt gcgcacccgt tccgggtcgt ggttgaactg gtgtccgtag
     5461 gggtagggac tgatatgggt gttgtggagg aaagcctctc cttcttcaat ctgcacgtag
     5521 ctatccttga gattcccctt gcccaggcgg agagacttca cttccgtccc caccagtgca
     5581 atccctgcct cgtaggtctc ttcgatgaaa taatcgtgat gggctttctt attctggcaa
     5641 aggagttttt cccccatgtt atattttata tcattaaccc ctttctttgg gcaacggaat
     5701 ctgaatggga aaaagccttg acaaagagaa cttgctgaaa gtaaacttat ttcaatagta
     5761 aacccttgtt ttctaatggt ttcattaagg agcgggaaat gacccgcttc aattatttcc
     5821 accccaaacc atggaaactg tgctttcccg ggtcttcatg atcaaaaaca agttcggcct
     5881 gcacgcccgg ccctccgctt ctttcgttca gaccgccagc cggttccgga gcgaaatcaa
     5941 agtggaaaaa gacggtctgg tagctgatgg caagagcatc ctggatttga tgatgctggc
     6001 tgcggcccag gggactcagc tcaccataaa agcccgcgga gcagatgccc aggaggcgct
     6061 aaaggccctg ggggatttga tcgaggggcg gttcggagaa gattaatttg acccgcgagc
     6121 ccaaaaagac ccaagtcatc aaggggatcg gagtcactcc ccaaatcatc atgggcaagg
     6181 cttacctggt ggaccgggga aagatcgaag ccccggcccg ggtgctgccc gagaaccaag
     6241 tcccctggga gatcgagcgc ttccgggatg ccgtggagga atccaaaaag cagctgcgtt
     6301 cggcaaaaga gaagctcctg cacgaggatg ccgtgggtcc cacttacatc ctggatgccc
     6361 atctgctcct tctggaagac aagaacgtca tccaaagcac ccgggaaacg attgaacaga
     6421 gctggattaa ttccgaatgg gcgctcaaga tcaacctgga ccgtctccgc aagattttcg
     6481 acagcatcga cgatgactac ctgaagagcc gaaagaccga cctggactac atcgaacaac
     6541 gcatcctgcg caatttgacc ggccggggca ccgaggcact ctcgcaaatc aaggaagagg
     6601 tcatcgtggt cgcccatgac ctttccccgg cggacaccgc ccagatggcc aaagacaaaa
     6661 tcaccgcatt cgtcacggac gtgggcggaa cgacttcgca taccgccatc atggcccgtt
     6721 ccctggagat cccggctgtg gtgggatcgg aaacggccac tcattccgtc aataccgggg
     6781 atacgctgat tgtggatgga atcaccgggg tggtggtggt aaaccccagc ccggagatca
     6841 tccaagaata catggaccgg aaccggaccc ggcagaggat tgaacgggaa ctgttcctgt
     6901 tccgagacct gccggcggaa acgctcgacg ggttccgggt caacgttctg gccaatatcg
     6961 agctggtgga ggagattccc gcagtcttgg accatggggc tgaaggaatc ggcctttacc
     7021 gaaccgaata cctgtatttg aatcgcaagg aattgcccac cgaggaggac catttccagg
     7081 cttacaaaac ggtggtgaaa gccatcgccc cccacccggc ggtcatccgg accctggaca
     7141 tcggcgggga caagttcata tcccacctcg agttggccga ggagatgaac ccggccatgg
     7201 gcctccgggc catccgtttc agtctgaaag aaccggagat tttcaaggtc cagctccgag
     7261 ccatgctccg ggccagcgtc tacggaaaag tgaagattct tcttccgatg atctccgggg
     7321 tgcgcgaaat ccgggaagtg aaaaagaccc ttaatgaagt gcgcctgagc ctcaccgctg
     7381 agcggatccc ctatgacccg aaggttgaaa tcggaatcat gatggaggtt ccttcggcgg
     7441 ctaccatttc ggacattctg gccaaagagg tggatttttt tagcatcggg accaatgact
     7501 tgatccaata caccttggcc atcgaccggg tgaatgaata cgtaacccac ctctatgaac
     7561 cccttcatcc ggccatttta cggctggtcc gccaggtggt gaaagccgcc catgacgccg
     7621 gcatccgggt ggccatgtgc ggggaaatgg ccggggaccc gctttacgtc ccggttctct
     7681 tgggcctgga actggacgat ctatcgatga acgtcctttc catcttccgg gtcaagaaga
     7741 tccttcgcgc ctacaccttg cgggaatgca aagaactggt ggaagcctct ctcggcctct
     7801 ccactcccga ggagatcgaa gcgctggttc gggccagtct gcaccggaaa ttccctgaag
     7861 aattcaggga gctttttaat tccaagcttt ccggaaactc ataaaaaaga ggagcaaaga
     7921 caaactatgg ggatgacaga tttcttgttc acttccgagt cggtcaccga aggccacccg
     7981 gataaggtcg ccgatcagat atccgatgcc gttctggatg ccatcatcgc cgaggatccc
     8041 aggggaaggg tcgcctgcga aactctggtg accaccggcc tggccatgat cgcaggagaa
     8101 atcaccacct cctgttatgt tcatatcccg gacatcgtac gagaaacaat caaggagatc
     8161 gggtacaacg actcggccat gggatttgac tgggagactt gcgcggtggt gacttccatc
     8221 gaccgccaat ctcccgacat cgccatgggg gtgaacgaaa gcccggatca tgagcagggt
     8281 gcgggagacc agggtttgat gttcggatat gcctgcagcg agactcccga gatgatgccc
     8341 ctgccgatcc ttctggccca tcgactcacc cggagaatgg cggaggtccg caagaagaaa
     8401 attctgaatt tcctgcgacc ggacggcaaa tcccaggtaa cgatacaata tgtgaacggg
     8461 aaagccatcc gcgtggatac catcgtcctg gcggctcagc atatcccgga agtgaagtac
     8521 gccgcgctgc aggaagggtt gatcgaagaa gtggtgaaaa gagtcgttcc ggccgaactt
     8581 agggaccaac ggaccaagta ttacctgaat gccaccggga ggttcgtcgt cggcggcccc
     8641 cggggcgatt gcggactcac cggccgaaag atcatcgtcg acacctatgg tggggtgggt
     8701 agccatgggg gtggatgtct ctccgggaag gacccctcca aagttgatcg cagcggatcg
     8761 tacatggctc gctacgtggc caagaatatc atcgctgccg ggctggcgga aaaagtggaa
     8821 gtccagattg cctatgccat cggcgtggcc cagcccgtat cggtcatggt cgacaccggc
     8881 ggaacttgga agattccacc cgatgacatc gccaagctcg ttcgagaggt cttcgacctt
     8941 cggccctcac cgatcatccg aaagttaaac ctcctccgcc ccatattcaa aaaaaccgcc
     9001 tgctacggcc acttcggccg caacgacgag gatttcacct gggagcggac cgatatggcc
     9061 gagacgctgc ggaaggcggc caaactttaa aaaaatttgg gacgcagatt ttcgcggata
     9121 cacgccgaga aaggtctttt ccggaaaatc ccaatcccct ttcggcgggg ctcgttcccg
     9181 atttccggtg tcttttttga acttgaccct gaaaatcggc gttcatctgc gtccaataga
     9241 aatggagagg aaatcgtgga ctacgacgtc aaagacatcc acctggcgga gaagggaaag
     9301 atgagggtcg aatgggccat gaaatccatg cccgtcctga agctcatcca gaggcggttc
     9361 cagcggcaga aaccgctgaa gggggtgcgt ctggccgcct gcctgcatgt gaccacggaa
     9421 accgccgccc taatgatgac cctccaggcc ggaggagcgg aggcccgggt ttgcgcctcc
     9481 aatcccttaa gcacgcagga cgatgtagcc gccttcctgg ccaaagacct gaaggttccc
     9541 gcctatgcca tcaaggggga agaccataaa acctattatc aacacatcca ttcggtcctg
     9601 gacctgaagc ccatgctgac catggatgac ggggcggact tggtgtccac cctgcacacc
     9661 gagcgcagag agcttctccc cggactcaaa gggggaacgg aagagacgac cacgggcgtc
     9721 atccgccttc gttccatggc cgacaagggg gttctgcgct acccgatcat cgccgtgaac
     9781 gacgccaata ccaagcattt tttcgacaac cgctacggga cgggccagag caccattgac
     9841 ggaatcatcc ggtccaccaa ccgcctcatg gccgggagcg catttgtggt ttgcggatac
     9901 ggatggtgcg ggcggggcgt ggccatgcgg gccaagggaa tgggagccaa cgtgatcatc
     9961 gttgaagtgg accccctgag agccctggaa gcggtgatgg acggatatcg ggtcatgccc
    10021 ataggggagg ctgcttcggt gggggatatt ttctgcaccc tgaccgggga catccaggtg
    10081 attcgccaag agcatttctt aaagatgaaa gatggggcca tcctggccaa ctccgggcac
    10141 ttcaacgtgg aaatcgacct ggaggcgctg gaacgaatat ccaccggcag aaggaccatc
    10201 cgcgatttcg tggaggagtt ccggctgagg aatgggaaaa aagtttatgt gctgggggag
    10261 ggccggctga tcaacctggc cgcggcagag ggacatccct ccagcgtcat ggacatgagc
    10321 tttgccaacc aggccatggc tgcggagttc atcgtcaaaa gccggggaaa actggccaag
    10381 aaggtttacg gggtcccgcc ggaaatcgac caggagatcg cccgcctcaa gctggcgtcc
    10441 atgggaatca aaatcgacac cctgaccccc cgccagaaaa agtacctcaa ttcctgggag
    10501 atggggacct gaaaaacagc gatcagcgat cagctttcaa ctgtcagccg gaggccatct
    10561 cagagatatc tttttggctg aaagctgacc gctgattgct tcttagtgcc tcaccgacaa
    10621 cagcagatac actcccacca tggcaaagat gaagtttccc agccaggctg ccaagaaggg
    10681 gggaaaggtg cctcccttgc cgagttccag gataaaagaa tataccacca ggtaaatgaa
    10741 gctgatgaaa atgctgtagc cgagcccccg ggcgaaaccg gctccccgtt cgcttcgcag
    10801 agccaaggga atccccaagc aggccatgat aaccacggtg aaggggaagg aaatggcgcc
    10861 ttgcatggct gtccgatatt ttcggctgtc gtagccgtcc tgctcgatct tttgcacgta
    10921 ttctctcagt tgtcggaaat tcatctcatc ggggtttttt tcggcaattt gaaaatcgct
    10981 gggcgtttcg ggaagggaaa ggatcagttc cgggtagtag tttcgagccg gtaccccttt
    11041 ccggtcaaaa tcggttaccg atgcctcgga cagaatccat tggccctctt tccactgggc
    11101 gctgcgagcg tcaatccgtt ggatgaggtc ataatccggg ccgaaacggt agatggtcaa
    11161 gccctcgagg acattcctat tcggatgata gagctggatg ttatagatgg actgacggcc
    11221 atggatccag acctggcttt gcttaaaggt ggccactggg ttcttcttat ggataatgaa
    11281 gtcggagatg gaccgggcgt tctggttcgc ccagggcatg atgaattcgc tcagccccag
    11341 catgccccca aaaaccagga tcaccacgcc caggacgggg taaaccagaa atcccaggct
    11401 gacgcccccg gatttcatgg ccatgacttc gttgtgcctc gccatgaggc ccagggtgat
    11461 catggaagcg agaaggacgg cggcggggga cacctggaag aggatcgagg gaatcttatt
    11521 caggaaatag gcacccatca agggccaagt ggccttgtta aaaaggaaaa cgttgatccg
    11581 ttcaaaaaat tcgatgatga ggtagatggc gctgaagccc ccgagtccga tggagaaata
    11641 ctgggcgaat tctcgggcca ggtagcgggt gatgaccttc atcccggaat ccctccgcca
    11701 tgctgacgct tcagccgcag ccattcgatc cccttttgaa cccagacgaa tataagaacc
    11761 ggggattcat tggccgcttt gaccagaagg tgaatgccca ggacgagaaa aatcaagttg
    11821 ggtgcccaca tccccacctc aaggaagatg gttccccttt cggccaagga ggtcccagca
    11881 ctgataaaaa ggtaatagat caggagtatc ccgatggacc aggaaagccc ccgggacttg
    11941 ctcccccgcc ctgcccttga ctggagcccc agggggacac cgatgatccc gaagactagg
    12001 caggcgaagg gaatggagaa cttttcatgg actttcaccc gttgagttct ggtatcctcg
    12061 gccttggcct tgagggagtg gatggattgg atcagctccc ccaaggtcat gtccgcaggg
    12121 tgtttctctt cggttttctt ttcctgaagc cccgtcttga gatagaggcg aaggtcatag
    12181 gtgttgaatt cgaccttctg gtaagcatga gggtccgttc ccagcttatg gatggacccg
    12241 tttttgagcc gtagggtgac ggcaagggcc tgcggatcgg aaagcacctc cccttcccgg
    12301 gcaaagatgg taaagacctc ggccggattg cgtgaatcgg agatgaaaac gttttcccaa
    12361 gtcaaggtct tgggcttcac ctgttctata taaaggacca acccatcgaa gtcgtcgttg
    12421 aagacccttt cccggactcc cgcattggct ctggtgctgg caacttcgaa caggagggcc
    12481 cgggaagcct cattggccag gggaacggcg tagagggaaa ggagggtggt aaccgcatag
    12541 gtgataaacg caaagataag aatcggggga atcatctgat agaggctaat ccccgaggcc
    12601 ttgagggcgg tgatctcgtt atcgcccgag aggcggtgaa aagtcagaag gactgaaaga
    12661 agagtggcca tagggatggt cagacttaga aattggggaa ggaggaggta gagcaggcgg
    12721 aggatgtatc ccagggggac gcccttattg acgaatagct ccgtaagctg taaaatcctt
    12781 cccgtaagca agacgagggt aaagccgaag aggccgagca aaaaggcggg aacggtttct
    12841 ttaagaagat agatatataa agttcgtttc atgtcatgat cttagcctaa gggcaagggt
    12901 tttgtaaatc cctccctctt ctgccggagg gggggttccg aggatgaagg atgtccattc
    12961 gtagaatgct ggaagaaaga gaagcgaaga ttttttcccc ctacgcccaa ctcagttccc
    13021 aatcccgcgg gagagcggtg cctgaggccg aatgcagtat ccgccctgct tttcagcacg
    13081 accgggaccg gatcctccat tcgaaatctt tccgccgcct caaacacaag actcaggtgt
    13141 ttttggtccc cgggggagat cattaccgca cccgcctcac ccacaccctg gaggtggccc
    13201 agattgcccg gactatggcc aaagccctgt ctctgaacga ggatttgacc gaagccatcg
    13261 ccctgggcca cgacctgggg cacactcctt tcggccacgc gggagaggag accctcaacg
    13321 agatttgccc gggaggtttc cgccattacc tccagagcct gagggtggtc gaccatctgg
    13381 aaagagacgg gagagggctc aatctcactt tcgaagttcg ggatgggatc gtgaagcact
    13441 ccaaggggaa gggggacttg atccccgatg tcccggaaga actgccggtt acctatgaag
    13501 ggcggctggt acgagtcgcc gacatcatcg cctacatcaa ccacgacctg gatgacgcca
    13561 tccgggcggg attgatcttt cccgacagca ttccccggga ctgcgtcgaa gaactggggg
    13621 attcccacgg aaagcggatc gacagcatgg tccgggatat tctggaacag gccatgaaag
    13681 gcgattccct aacgatcgga ttgagtgaaa gaaagctgga ggcgacgctg aaactccgcg
    13741 attttctcta cgaccgggtc tatgacctgc ccacggttca cggggatttt gtcaaatcgg
    13801 ccaagatcct gcgggagctt tatgcctact tcctggatca cccgcaggag tttcgaatgg
    13861 aggcagggga ggcctttagt cacgaacccg tcgagcggtc gatcactgac tttatcgccg
    13921 gcatgaccga ccgctatgct ttcaagctct atgagaagat attcctcccc cagccatgga
    13981 tgattatttg accgggaaaa ggctagtgtc gcgtctcaga aataagctgc ataattctgt
    14041 ccccgagcag ggagcggggc gaagaaccgc tgcgcttgcc aaaataagaa gctcgttcct
    14101 ctttgaaaaa gttgattctt actaaaggtt tcgggagttg tctatttttg cgctcttggc
    14161 ataattcccg gaggccggca gcgtcgcttt gcggatacca ggccactgct cggggatagg
    14221 ccatttccgg gccgaacgag cgactggcaa cgatcttttt ttcgagaagg cacaaggaag
    14281 cagcgacggc tcttgaaatt tagtaatatt tgtggtacat gatcttcatt ttagggggac
    14341 cctatgactg acaaaaagga tgagccgatc attgaattga ccgaggttgt ggaagaggct
    14401 ccagagtctt caggggggcc ctggatggaa aattcccatc ctcccgcccc tcctccggct
    14461 cagcagataa gggaacccga atctcctccg gttcttccgc gggagaatgc ccaaaaaatg
    14521 gatgaggcgc tgaaaaggat accggattcc cccctaaaga aatttatcct ttcggaagat
    14581 gaaaccaccg ggatcctacc gccgccggca aaagcaccag aatttccaac cctctccccg
    14641 cagccgccga ggatttcgcc cgagcccgcc aaaccaacgc ccgcgccgaa ggttacgttc
    14701 cccgaggccc caagaccgca cccagaaccc cccccaatgc cgcctgaacc tgtgcaggcc
    14761 ccttctgaac cccccaagtc gcccgttatg gatgtggaag cggagcttcg aatcatccgg
    14821 gaagccatgc tttcccgggt ggagagatgg gtatcccagg aaggggttca ggctttggag
    14881 cgggtggccc gggagatttt tcccaagata gctgaagagg tcctgcgcaa ggaaatcgaa
    14941 aagatcaaag cggaagccga ggagaaggag tgaaagaaga agaggcgaag gaatggagta
    15001 aaatttacga cccccaccga gtggaagata aatggtacac cttctgggag aagagaaatt
    15061 tctttcgggg ggatgagaat tcgaaaaaaa cccctttctc catcgtgatc cctcctccca
    15121 atgtaaccgg ctccctccat atggggcatg ccctgaataa cacccttcag gacgttttat
    15181 ccagatacaa acgcatggac ggtttcaacg tcctctggct gcccggcacc gaccatgccg
    15241 gcatcgctac tcagaacgtg gtggaaaaac agctggccga ggaggggacc gaccggcatc
    15301 agctgggccg cgaggctttc atccagcggg tatggaagtg gaaagaacag ttcggcgggc
    15361 acatcatcaa acagttgaag aaactgggga gttcctgcga ctggagccgg gagcgcttca
    15421 ccatggacga gggactttcc cgggcggtcc gggaggtctt cgtccgcctg taccaggagg
    15481 gtttgatcta ccggggggat tacatcatca actggtgccc ccgttgccag accgcccttt
    15541 cggacctgga agtggagcac gaggatgact cggggcacct gtattacatc cagtatcccg
    15601 tggatggatc gcaggaggcc ctgaccgtgg ccaccacccg gccggagacc atgctcggcg
    15661 acaccgccct ggcggtgaat cccgaggatg aacggtataa aaaatatgtg ggccggaggg
    15721 tcatcctccc cctcatgaac cggaagatcc cagtcatcgg cgacgggtac gtggcgctgg
    15781 atttcgggac cggagttttg aaagtgaccc ccggccatga ccccaacgac ttcgagatcg
    15841 gccggagaca caacctcgcg gtggtcaaag tcatcgacga ggcggggaac atgagccagg
    15901 aggcggggaa gtactcgggc ctgggccgtt tccaggcccg cgacgaggtg gtcaaagacc
    15961 taaaggccct ccacctgctg cagaagatcg agccctttcc ccacagcgtc ggccactgtt
    16021 accggtgcaa aaccgtcatc gagcccctgg tttccaagca gtggttcgtg aaggtcaaac
    16081 cgctggcgga gcgggccatc gccgcagtgg tgaaagggga aacccgaatc atcccccagg
    16141 cctgggagag tacctacttc gaatggatga acaacatccg cgactggtgc atctcgcggc
    16201 agatctggtg ggggcaccgg attccggcct ggttctgcga aaaatgcaat gaagtgatcg
    16261 tatccatcga gccggtgacc caatgtccgg cctgcgggag ctctgccgtg cggcaggaga
    16321 cggacgtcct ggacacctgg ttcagctccg ccctctggcc cttttccacg atggggtggc
    16381 cgaagaagac caaggacctg gaggtctttt accccacctc ggtcctggtc accgggttcg
    16441 acattctttt tttctgggtg gcccggatga tgatgatggg gttgaagttc atggaagagg
    16501 ttcccttccg ggaggtttac atccacgcct tggtgaggga cgccgaagga cagaagatga
    16561 gcaagtcccg gggcaacgtg atcgaccccc tggaagtcat cgagaagttc ggcaccgatt
    16621 ctttccgctt caccctggcc gcttttgccg cccaggggcg ggacatccgt ttgtccgaag
    16681 accggatttc agggtaccgc aacttcgcca ataaaatctg gaacgcatcc cggttcaccc
    16741 tcaccaacct ccagggcttt aatccggaaa agtccccggg aaagaccgaa ttgactttgg
    16801 ccgacaagtg gattttgagc cgggtcaacc gggtggtggc tgaggtgcgc gagggcctgg
    16861 acagctacaa attcaacgag gccgcttcgg ctgtctacca atttctctgg catgagttct
    16921 gcgattggta catcgagctg atcaaaccgg ccctctatca ggatcaggac cccggccgaa
    16981 agtggctaac ccagaatgtg ctggcccgag tcctggatac ttctctgcgc ctcctccatc
    17041 ctttcatgcc cttcattacc gaagaaatct ggcaaaatct tccgggaaac aatgggtcca
    17101 tcatggtgac ggagtttccc cggattcagg agaacgaagt caacctccgg gaagaggccc
    17161 agatggaatt gatcatgaaa gtaatcgggg cgatccgcaa cctgcgcagc gagatggaga
    17221 tcgcccccgg gaaaaaggtg gaagcaatcc tttactttcc gaaaaaggaa cccctgcatt
    17281 tcctggagga aaaccgctcg tacctggaaa atctgggccg aaccgagaag ctcggattgc
    17341 agtccggggg gcagaggccc aaagcctcgg ccacggcgat tgtggatgaa gtcgaggtct
    17401 ttctgccctt gaaagggctg atcaacctgg acgaggaaga aaagcgggtc cagaaggaat
    17461 tggccaaggt ttttgaagac ctatcccgag cccggggaaa actccagaac caggatttcc
    17521 tgagccgggc ccggccggaa gcggtagaaa aggaaaaaga gaaggccaga gtcctgtcgg
    17581 agaaagaggc caagctcaaa gaaagcctgg agagagtgaa gggctggagg aaagaaacct
    17641 aaatgattgc ggattttgga tttcggattg cggattgagg tacggccaga agatttagtt
    17701 tttaatccga aatctgcaat ccgcaattta aaaagggggt tgccatgtat tttcccctct
    17761 accgtccccg tcgcctgagg aagagcgaag ccttgagaag gatggtccgg gagaccaagc
    17821 tctctccgga ggatttcatc tatcccctat ttatcgtcca cgggcgcaag gccaggcacg
    17881 agatcaagtc catgccggga attttccagc tatccattga cctggcggtg cgcgaagcgg
    17941 aagaggtctg caaactgaac atcccggcgg taattctttt cggcattccg gagaggaaag
    18001 acctcaaagg aacccaggct tacgcccccc gggggatcat ccagcaggcc atccgggcca
    18061 tgaaacggga gcttccggac ctggtggtca tcaccgatgt ctgcctctgc gaatacacca
    18121 gccacggcca ctgcggggtg gtggtcgacg gcgaagtgca caatgatgct actttgaatc
    18181 tcctggccag gatggccctt tcccatgccg aagcgggggt cgatatggtg gccccttcgg
    18241 acatgatgga cggccgggtc aaagccatcc gggatgtcct ggaccggaaa gggtttgaag
    18301 atatccccat cctttcttac gcggccaagc acgcctccgt tttctacggc cccttccgcg
    18361 aagccgccga gtccaccccc cagttcggag atcgccggtc ctaccaaatg gacccggcca
    18421 atgcccgcga agcgatccgg gaagtgaggc tcgacgtcca ggagggggcg gacatcatca
    18481 tggtcaagcc ggcccttgcc tatctggaca tcattcggaa agtgagggag gagattgaat
    18541 tgcccgtggg agcctacaac gtgagtggcg agtactccat gatcaaggcc gcggctcagc
    18601 ggggatggat cgacggagaa cgggcgatga tggaggtgct cacggccatt aaacgggccg
    18661 gggccgacct catccttacc tacttcgcca aggaggccgc gcggttcctg gaaaagaacc
    18721 gatcggcgtg ctaagttcgc gcgctccgcg cacgaagatt gcgtcactct ggtttcaaca
    18781 ttgatgatct cgcaaaagtc tcttggatcg tcaccccggc gaaccccgga tcaggtccgg
    18841 ggcaggcgcc ggggtccagg aaatattgaa agacctggat tccggctttc gccggaatga
    18901 caaatttcgt gggattttaa ctttcacagg attatcaacc ttcggcatgc ggaagttcct
    18961 cttcccccgg aaagggggat gatgggttta ccattggagg atcccccccg ggggggccag
    19021 caaagggagt ccaaaccatc tctagccagg aagggaggaa aaaccgatga gggaaagaaa
    19081 aaagatcagt cgtagagcat ttttaaagac cgccgcggca ggaatcggcg gaatcaccct
    19141 ggggctaaag ggaatcgagg cttctgccca ggataagacc ccggccaaag ccaaccccgc
    19201 cagaaaagcg ctgggcgtgg atgtcatcaa gcttggggga cagggggtca tctccggggc
    19261 ccatgccgat tacggctggc agatccaggg cggggccact ttggccatcg aggagatcaa
    19321 tgccaaagga ggggtcctgg ggagcaagct ggagcttaaa ttcatggatg aggagctcaa
    19381 gcccgcggtg gccgtgaaaa atgccaggta cctggccacg gaatggggcg cccatcttct
    19441 cttcggagtc gattccagcg gcagcgccat ggccatcggc cccatccttc ccgagttgga
    19501 tcggatctgc atgttcgacc atgcctccac ccaccggcta accgaggaat tggtgtatga
    19561 aaaaggaatc aaacagatct tccgcatcgc cgccccagtt taccaggagg gcaacctggc
    19621 ggccatgatt ttcaaggagt ttcccaacat caagaggtgg gccgggattc actgcgacta
    19681 tgaatacgga tattccagct gggccctctt caaagatacc ttgaagaagt accgcccgga
    19741 tgtggaattt gtggccatcg cctgggcccc cttctggacg atggattttt cctctcacat
    19801 ctcggcggtg atggccgcca atcccgatgg catccatgcc accccgtggg ccggcgaagg
    19861 ggtcatgctc ctccggcagg cgctcatgct gggggtcttc gacaagatcc aggcctggtt
    19921 ccaatgcatg ggggcctccg tggatatcct cgaagggatt tccaatgaag tggcggcggg
    19981 caaattcaag gataaactgt gggccacggc ccgttatatt cataactacc ccgatacccc
    20041 ggagaacaag aaattcgtcg aagctttccg caagcggtgg aaccgctttc ccaactactc
    20101 cgcggaaaat gcctactcca ccatttatgt cttcaaggcg gccttggaaa agtccagaag
    20161 cctcaagacc gccgacctcg tgaaggccat agaaggaatg aagatcatga cccccgcagg
    20221 agaaaggttc ttccggcccg aggaccatca ggcgatttac gccgtgcccg gaggcagggt
    20281 ggtgaaagac cccaattatc ccattcccgt cctgggtgac ctgaaggtgt tccccgccca
    20341 ggcctactat cgaaatcccc cttttgaacc tctgaaaatg ggtaaataat tccgccggga
    20401 gagccggacc ttaccggctc tccctctttc aatatcgaag gaaaagagat ggagacgtta
    20461 gccttccagg gactcgtagg cctgagcgtg gccatgtacc tttggctcct ggccgccggc
    20521 ttgaccatcg tgttcggcgt gctaggggtt ctcaatttcg cccacggcag ccttttcatg
    20581 ctgggcgcct atttcgcctt cacattctac ggcctgtggg gggtcaactt ttggctggcc
    20641 attgccttga gcctcgtgtc ggttgccgtc gtgggctacc tcatggagcg ttttttccta
    20701 aggcacatct ataaccagga ccatgcctac cagcttctct tgaccttcgg gttcatcctt
    20761 atcctcgatg acgccgtaaa gatcgtctgg ggaggggtgg ccaaaattcc cgccatgccc
    20821 agtttcttcg aaggaacggt gtcggtggtg ggaaggccgt tcccggtcta taacctgttc
    20881 atcatcggcg ccggcgtgtt cgtggccatc ctggtctggt tgctccttga aaagacctgg
    20941 tggggaaaga tggtgagggc ctccgcctcc gaccgggaga tggccggggc tattgggatc
    21001 aatatttcca ccctcttcac cggcgtcttt gcctttgggg ccatactggc tgccctgggg
    21061 ggagccctgg ggactccggt tcgggtggtg gctccgggga tcggcgcggc tatgatcatc
    21121 caggctttca tcatcacggt gatcggaggc cttggaaatc tgaagggggc tttcgtgggc
    21181 gctctcatcg tgggaaccct cagcgcctat gggatcctgc tcttcccggt ctttgagctc
    21241 tttttgatct tcctgatcat ggccgtggtc ctcatgatca agccgcaggg actctttggg
    21301 agctgagcga tgaaaaccag actggcgatc ctgcttctca ttatcttcct ggcttttctg
    21361 ccggcctttg cggggaaata ccttctctac gtcaccattc acatcctgct tctttcgatt
    21421 ttttccctgg gattcaacct tctcttcggc tataccggcc tcctgtcctt cgggcaggcg
    21481 gggttctacg ccgtgggggc ttacggttgc gccaagatcc tgctggtata cccgtcgctc
    21541 ctggcgggga tcctcggggg agtggccctg gccggggtcg cttcccttct gctaggcttt
    21601 ctgtgcatac ggcacacccg catctatttc tccatgctca ccctcagctt cggcatgatg
    21661 atcttttccc tggcctggaa gtggcgggat gtgaccggcg gggatgacgg actggtgggg
    21721 atcccccggg caccgctgga ggttccgggg gtcttcaaga tcaacatggc cgccatggag
    21781 agctattact acgtagtcct gggtttgtcg ctgctggcca tttatctctt cctgcggatc
    21841 gttcgttccc ccctggggtt gaccctgcag gggatccggg acagcgaaac ccgcgcggcc
    21901 tgcgtcgggg tctcggtccg aaaataccgc cttctctcct ttaccatcgc cgggctttac
    21961 gccggcttgg ccggaaccct cctccccccg ctggagagca cggtgactcc tcccgtggct
    22021 cactggagct attccgcgga gcccgtactg gccaccttgc tgggaggaat ctacagtttc
    22081 tccggaccga tcgtggggtc cgttctcttc taccttttaa aggacatcat tgtgcgcttc
    22141 acggagtatt ggctgatctg cttggggatc attgtcgtgg tgctggtcat gggattccgg
    22201 ggaggggtgg tgagcatttt cctgaaagag gggagccggc gttgaaagaa atcatccgca
    22261 ccgagggcct agcaaagtat ttcggcaaga tggcggcggt ccacgaggtt accctcaccc
    22321 ttcaggaggg cctgttcact tcggtcatcg gaccgaacgg ggcggggaaa accacgctca
    22381 tcaaccttct gtccggctat ctgcttccgg accggggcaa ggttctattc caaggaagcg
    22441 agatcacccg gctgggtccg gacaagaggg taaagatggg gatcagccga tccttccagg
    22501 tgatgaatat cttcccgagg atgacggtct tccaaaatat cctgctgccg gttttgtccc
    22561 gcctcaataa gacctccagc gctttctctt ccctcctgag ccagaaagaa gcggtggggg
    22621 aggcggagag aattctggtt gaagtctggt tgaaggacga aaaggatttc cccgccgggg
    22681 aactgtccca cggcgaccag aggcttctgg aactggggat tgcggtagcc agcaatcccc
    22741 aggtctgttt tctcgacgag ccttcttcgg gcatgaaccc catcgagcgg atcaaagtcc
    22801 tggagctagt caagaagctg gccgcggagc ggaagaccac cttcatcatc gtggaacacg
    22861 acatggatat tgtcttttcc ctctccgact ggatcgtggt tatgaaccgc ggggagattt
    22921 tggcggaggg ccggccagac gagatccggg ccaaccggga ggtccgggag atttacctgg
    22981 gagaggaagt atgaccgccg tcctggaagt caaggagatc atcacccgct atggacaaag
    23041 ccaggtgctc cacggagttt ctctggaggt ggggcagggc gaagtggtag gcctattggg
    23101 gaggaacggg gtgggaaaga ctaccacctt ccgttccatt atgggactga ctccgccccg
    23161 ctccggaagc atccggctca aaggggtgga gttggggggg aagaaatcct acgagatcgc
    23221 ccggttggga atcggatatg ttcccgacga ccggaggatc ttccctgacc tgacaaccga
    23281 agagaacctg gaaatggccc ggcgcctggc gaagaagggc gaaggaccct ggaccttggc
    23341 gcgggtttac caaacctttc ccgttctgga aaagctgaag tccagcaaag ggacgacgct
    23401 cagcggggga gagcagaaaa tgctggccat cgggcggggt ctcatgaaga accccgaaat
    23461 tctgctcctg gacgaaccct cggagggtct ggcccctctg gtggtcagaa acctgaccga
    23521 aatcatcctg cagattcaaa agtccggagt caccctgctg ctcgccgatc agaacatcaa
    23581 attctgccgc aaggtcgccg gccggggcta catcatggaa aaaggcgtga tccagtatga
    23641 aggggccatg gatgatatct ggcagaa
//
LOCUS       DASPRR010000340         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1205828, whole genome shotgun sequence.
ACCESSION   DASPRR010000340 DASPRR010000000
VERSION     DASPRR010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205828"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1151
                     /locus_tag="VEL68_12900"
     CDS             174..1151
                     /locus_tag="VEL68_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36909.1"
                     /translation="MKSRRRLPCAAFLVVISFLAGGQAMGAETFPGKPLELVVPYAAG
                     GSTDVMCRTLVARAAPFLNNQPVIVVNKAGAGTVAASKYVLDGKSDGYTLYSTSTSSM
                     MIAPVVNKGGYSWKDFSGIAQVMLGADAFWVRTDFPYNTIEKFIEYARQNPGKIKYSS
                     PGVGTTNHLAMEGFAAAKGFVIKHIPTKGDAEAVTAILGGHVVAAAGNAITYQPHGDA
                     GKIKCLAQFGGVRDKAFLPHIPTFKEMGMDVVVDLWRWIVVPKAVPPERVKTLADAFQ
                     KVLQDKETLAALEKIQCPVSYVRPEGYEKVMQDSEATILPLIKLTKLDK"
     gene            1345..1566
                     /locus_tag="VEL68_12905"
     CDS             1345..1566
                     /locus_tag="VEL68_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ36910.1"
                     /translation="MSPGKKRKAKVILIQEKWCKGCGICVEFCPGKVFSSQPGQVPSV
                     ARLGDCTVCRLCEMRCPDYAVSVEEVSGP"
     gene            1563..2693
                     /locus_tag="VEL68_12910"
     CDS             1563..2693
                     /locus_tag="VEL68_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ36911.1"
                     /translation="MKGNERLMMGNEACAEGAIAAGVRFFAGYPITPASEIAEIMSVR
                     LPQVGGCFIQMEDELASITAATGASLGGMKAMTASSGPGLSLKQEGIGCAAIMEVPVV
                     VINVQRGGPGIGNIQPSQGDVMQARWGSHGGPQLIALAPSSVKECFDLTAKAVNLAER
                     FRVPVIVLSDASVGHMREKISVPDSVEVVNRKRPTVPPDKYLSYDTDEPDGVPPLADL
                     GTEYLCHITSFMHHKNGFPAWTDLKVTDALVKRLANKILWHQEEIIALERYLLEDADI
                     ALVAYGSTARPAKAAAAMARKKGIRAGLLKLMTLWPFPGEEIRKISEKARTLMVPELN
                     MGQIVGEVARWACGKSRVVGINRVDGALLTPEEILARLEEDK"
     gene            2694..3536
                     /locus_tag="VEL68_12915"
     CDS             2694..3536
                     /locus_tag="VEL68_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ36912.1"
                     /translation="MAKSKSQTALLSQYFRQQILPTTWCTGCGIGTIMGSIVRAIEAT
                     EIAPHRFVFVTGIGCYGPAGQYIRISDLHALHGRTPSYATGLKLANPEVYPILLMGDG
                     DAASIGGNHLIHAARRNIDVTAIVMNNHNYGMTGGQYSPTTPQQVPASTAPYGMVERS
                     FDLCKVVQAAGATFVARTTVYHVSQMVNFLTRAILHKGFSFVEIESQCPTYFGRFSKR
                     GSPVAMLKWQKENSVPVEKAGGMKPEELEGKIITGIFVEEKRPEYVEDYYRLIAKVKE
                     KISA"
BASE COUNT          922 a          979 c         1136 g          806 t
ORIGIN      
        1 gacaaggcca cactggaaac cctgaccaaa cttcaatgct gggtggatta ccagcccctt
       61 gaggagtacg aacggctcat gggcaaaagt gaagccgccg tctccccctt gatcaaactg
      121 gccaagatcc tggatgagaa ataagttcgt ggatggaccg gaaaggagtg attatgaaga
      181 gtcgaagaag gttgccttgc gctgccttcc tggtagtgat ttcttttttg gcgggcggtc
      241 aagccatggg ggcagagact tttcccggta agcccctgga actcgtcgtc ccctatgccg
      301 cggggggctc cacggatgtg atgtgccgga cgctggtggc ccgggccgca ccctttctga
      361 ataatcagcc ggtgatcgtg gtgaataagg ccggggcagg gaccgtggcc gcgtccaaat
      421 atgttttgga tggcaaaagc gacggctaca cgctttattc aacgtccacc tcgagcatga
      481 tgatcgcccc ggtggtgaat aagggtggct attcctggaa ggatttttcg ggaattgccc
      541 aggtcatgct gggggccgat gccttctggg tgagaactga ttttccctac aacacgattg
      601 aaaagttcat cgagtacgcc aggcaaaatc cggggaagat caaatattct tcacccgggg
      661 tgggaaccac caaccacctg gccatggaag gttttgccgc ggccaagggt tttgtgatca
      721 agcacattcc cacaaagggt gacgccgagg cagtcaccgc catcctcggg gggcatgtgg
      781 ttgccgccgc aggcaatgcc attacctacc agccccatgg cgatgcggga aaaatcaaat
      841 gcctggccca gttcggcggg gtccgggaca aggctttttt gccccatatt cccaccttca
      901 aggaaatggg aatggatgtg gtcgttgatc tctggcgctg gatcgtggtt cccaaggcag
      961 tccctccaga aagagtcaag accttagccg atgccttcca gaaggttctc caggacaagg
     1021 agaccctggc ggccctagaa aaaatccagt gcccggtttc ttacgttcgg ccggagggct
     1081 acgaaaaggt catgcaggac agcgaggcca ccattctgcc cttaatcaaa ctaacgaaac
     1141 tggataaata acccgcaggg cggcagagtc gcaaccaaat caccccgtcc ctaccctccc
     1201 ccctcgaggg ggagggttag ggtgggggga tgctttttgc ttagaaagaa tttcagatac
     1261 tgaatattag tagcacagag ccggggcagg gcaaaattta tgaatgggaa aattggaatg
     1321 aaaaaggaga aaccagaaaa ggaaatgagt ccggggaaaa aaaggaaggc gaaagtcatt
     1381 ctcattcagg agaaatggtg caaaggttgc ggcatctgcg tagaattctg ccccgggaag
     1441 gttttttcgt cccaaccggg ccaggttccc tcggttgccc gcctgggtga ttgcacggtg
     1501 tgccgcttat gcgagatgcg ctgtccggat tatgccgtgt ccgttgagga ggtgagcggg
     1561 ccatgaaggg aaacgaacgt ttgatgatgg gcaacgaggc ctgtgccgaa ggggcgattg
     1621 ctgccggggt tcgatttttc gccggatacc cgatcacccc ggcctcggaa attgctgaaa
     1681 tcatgtcggt ccgcctccct caggtgggtg gatgtttcat ccagatggaa gatgagctgg
     1741 cgagtatcac tgcggccacg ggagcctccc tggggggcat gaaggccatg accgcgtcga
     1801 gtggacccgg tctctctcta aaacaggaag ggatcggctg cgccgcgatc atggaagttc
     1861 cggtagtagt gattaatgtg caaaggggag gtccgggaat cgggaatata caaccctccc
     1921 agggggatgt gatgcaggcc cggtggggct ctcatggagg tccccagttg atcgccctgg
     1981 ccccgtcatc ggtgaaggaa tgctttgacc tgaccgccaa agcggtgaac ctcgcggagc
     2041 gattccgggt tccggtgatt gtcctttcgg acgcttcggt ggggcacatg cgggaaaaaa
     2101 tttcggttcc cgattccgtg gaagtggtca accgcaaacg gcccacggtt ccccccgaca
     2161 aatatctctc ctatgatacg gatgaacccg acggcgtacc ccccctagcc gatttgggga
     2221 cggaatacct ctgtcatatt accagcttta tgcaccacaa aaacggattc cccgcctgga
     2281 ccgatttgaa ggtgaccgat gccctggtga aacgcctggc caacaagatt ctctggcacc
     2341 aggaggaaat catcgccttg gaaaggtatc tgctcgaaga tgcggatatc gccctggtgg
     2401 cttatgggtc cacggctagg ccggccaaag cggccgcagc catggccaga aaaaagggga
     2461 ttcgggccgg cctgttgaag ctcatgaccc tctggccttt cccgggagaa gagatcagaa
     2521 aaatctccga aaaagcaaga accctcatgg tcccggaact gaatatgggc cagatcgtgg
     2581 gagaggtcgc acggtgggcc tgtggaaaat caagggtagt gggcatcaat cgggtcgatg
     2641 gagcattgct caccccggaa gaaatcttgg cccggttaga ggaggataaa taaatggcca
     2701 agtccaaatc tcagactgca ctactttctc agtatttccg gcaacaaatc ctgcccacca
     2761 cgtggtgtac aggatgcggc atcgggacca ttatgggatc catcgttcgg gccattgaag
     2821 ccacggagat tgctcctcac cgctttgtgt ttgtgaccgg gattggctgt tacgggcccg
     2881 caggacaata catccggatt agcgaccttc acgctctaca cgggagaacc ccttcttacg
     2941 ccacaggcct gaagctggcc aaccccgagg tctaccccat cctactgatg ggtgatgggg
     3001 atgcggcttc catcgggggc aatcatttga tccacgccgc ccgtcggaat atcgacgtga
     3061 ctgcgattgt gatgaataac cataattacg gcatgaccgg gggccagtat tcacccacca
     3121 ccccccagca agttccagca tcgaccgctc cctatgggat ggtggaacgt tcctttgacc
     3181 tctgcaaagt cgtacaggcg gcgggggcga ccttcgtggc ccgaacgacg gtttatcatg
     3241 taagccagat ggtcaatttc ctcaccaggg cgatcctgca taagggattt tcctttgtgg
     3301 aaatagaaag ccagtgcccg acctatttcg gcaggtttag taaaagaggg tctcctgtcg
     3361 ccatgctcaa gtggcaaaag gagaactccg ttcctgtcga aaaggcgggg ggaatgaagc
     3421 cggaggaact ggaaggaaaa attattaccg gtatttttgt agaagaaaaa cgccccgagt
     3481 acgtggagga ctattatcgc ctcattgcca aggtgaagga aaaaatatct gcttaggctt
     3541 aaaatctgga aagggaatat aaacttagct cttttgaatt gccttctcaa atcccccctc
     3601 cgcccccctt tttcaaaggg gggaacgggg gggatttctg cagatgcctt gctcaaaaaa
     3661 aggtgtagag aaatatggtg gcttttctcg aagagctaga gtgatatgaa agagagaaac
     3721 caatgaagac cgaggtacga ctcagcggct cgggggggca ggggatgatc ctggccggta
     3781 tcattttagc cgaggctgcc ggcattcacg atgggaagaa tgtggtccag acccaatcgt
     3841 acg
//
LOCUS       DASPRR010000341         4784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_887876, whole genome shotgun sequence.
ACCESSION   DASPRR010000341 DASPRR010000000
VERSION     DASPRR010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887876"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VEL68_12920"
     CDS             complement(<1..876)
                     /locus_tag="VEL68_12920"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ36913.1"
                     /translation="MLLILFILFSLFMAFNAPISFSLGLASAITALMKGNVSLIIIPQ
                     KVFTSLDSFPLLAIPLFILAGSFLETGGVGMRLVNLANVLVRHISGGLGFVVIVSTIF
                     FSEISGSSAADAAAIGSVTIPAMIRLQYHPAFATAIVAASGGMAALIPPSIIGVIYGW
                     QANASVGAVFLGGFLPGFIMGGGLCVYTYFYAKKMNFPTEKRATRGEVWLAFKESLPA
                     LIMPVIILGGIFTGIFTATEAAAVAVVYGFIVALFYYRELKWGDIPRVLVESAVTTGF
                     VGLLLGLASVFGWVLT"
     gene            complement(894..1400)
                     /locus_tag="VEL68_12925"
     CDS             complement(894..1400)
                     /locus_tag="VEL68_12925"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ36914.1"
                     /translation="MKQVSLGENGGPMKILSWVRVIYQNPVGLACGVLMAAITAVVFL
                     QVVSRYVFSYPFDWPEELARYLFIWVALLGAALALKRGAHFSIDALVKRLPARWRLIV
                     PLLIHTSLGIFTLVVSVKGFQLALRVREQLSPGMEVSMTYAYLSVPVSFAIMFKYVVS
                     EVYSLFKK"
     gene            complement(1523..2503)
                     /locus_tag="VEL68_12930"
     CDS             complement(1523..2503)
                     /locus_tag="VEL68_12930"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ36915.1"
                     /translation="MKKGTWIFIAVGILLLGISFPGLSGAAEKVIKLGHVLDTKHPYH
                     IGSLHFAKRVAELTKGKVEVQVYPSSQLGNERELVEAMQVGTIEMGASTSAVAARFVK
                     EIEIFNLPFLFQGFPHLYKILDSQFGEEINQASQKKGLRILAWWVGGSRSIYARKPIS
                     DLASMKGMKIRTIESPVVVATWKALGLIPTPIPFGEVYTALQQGVVDAGEGNVISYES
                     MKFDEVAPYLSHIKYLITVQLLYIGENFFKGLPAEFQTALIQAGKECVPVERKANEEA
                     EEKIIELLKTKKKTVVIPNLDPFIAGVQPVYEQYGKAIGMDRIKWIQSYK"
     gene            complement(2573..3094)
                     /locus_tag="VEL68_12935"
     CDS             complement(2573..3094)
                     /locus_tag="VEL68_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HXZ36916.1"
                     /translation="MEGWSEEEIRNKDLMSPCGLYCGACGVYIATRDKNEKFKAVMGK
                     LYGTKPEETECLGCMQEDPPRKIYGYCKLCRIRNCVKGKGFYSCHQCKEWPCDLIKKF
                     GFGTGVKVMKRTIPIWRAKVAEHGDEKGSVEWARAECERYHCPSCGKPLFRGAQRCRE
                     CKRSVADELDGSL"
     gene            complement(3140..4234)
                     /locus_tag="VEL68_12940"
     CDS             complement(3140..4234)
                     /locus_tag="VEL68_12940"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HXZ36917.1"
                     /translation="MNRRRFLQNSALWGVSWALGGLISSCAQAPAKPVVDQAILRGLQ
                     IIDPHAHPDQFHTLRPGRIDDSSTLEKIRAAGMAASAFAAVGDLEFFNRTSATAYYPS
                     TKSQLQRVHDLVKAGKVKLVLKAADVPAIRNPGDPPGAIMAIEGGDPLDGKPERVDEF
                     YRMGVRIITVVHYRNNELGDVMVPYGALSPGPYNNGLTPAGQKVIERMQEVGMVVDVA
                     HAHLTTLKSIVEMNPNPLLDSHTNPCPIDEPRRCHRQRSWKDMERVAKTGGVVCTWPF
                     AFNRPDQKRRTFLDWAQEILEMKKRLGMDHVGLGTDGGGNIPRLIEGYRDVRDLSQLV
                     AAMQEVGLSPEDIKAYMGGNVYQLLLKCIG"
     gene            4549..4622
                     /locus_tag="VEL68_12945"
     tRNA            4549..4622
                     /locus_tag="VEL68_12945"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4581..4583,aa:Gln,seq:ctg)
BASE COUNT         1090 a         1404 c         1216 g         1074 t
ORIGIN      
        1 ggtcaggacc cagccgaaga cgctggctag cccgaggagg agacccacga aaccggtggt
       61 gaccgcagat tcaacgagaa cccgcgggat atccccccac ttcagttccc gataatagaa
      121 aagggccacg ataaaaccgt agaccactgc cacggcagcc gcctcggtgg cggtaaagat
      181 tcccgtgaag atacccccga ggatgatgac cggcatgatg agcgccggaa gggattcttt
      241 gaaggccagc cacacttctc cgcgggtcgc tctcttctcc gtggggaagt tcatcttttt
      301 ggcgtaaaaa taggtataga cgcacagccc cccgcccatg atgaaccccg gcagaaagcc
      361 ccctaggaag acggccccca cggaggcatt ggcctgccag ccatagatga ccccgataat
      421 gctcggagga atcagagcgg ccatgccccc ggaggcagcc acaatggccg tggcaaaagc
      481 gggatgatac tgcaggcgga tcatggctgg gatggtaacc gaaccgatgg ccgcggcgtc
      541 cgcggccgag gagccggaga tctccgaaaa gaaaatcgtg gaaacgatga ccacgaatcc
      601 cagccccccg ctgatatgtc tgaccaacac gttggccagg ttcaccagcc tcatccccac
      661 ccctccagtt tccaggaagg aaccggccag gataaaaagg gggatggcca ggaggggaaa
      721 agaatcgagg gaggtgaaga ccttctgggg aatgatgatc agggatacgt tacccttcat
      781 cagggcggtt atcgcgctgg ccagcccaag ggagaaagag atcggcgcat tgaaggccat
      841 gaaaagggaa aaaaggataa acaggatcaa cagcataacc aaacccgagg agcctatttt
      901 ttaaacaggg aatagacctc cgaaacgaca tatttaaaca tgatggcaaa gctgaccggg
      961 acggagagat aagcgtaggt catcgagacc tccatcccgg gagacagctg ttcacggact
     1021 ctcagggcca attggaatcc tttgacggat acgaccaaag tgaagattcc cagggaggtg
     1081 tggatcaaaa gcggaacgat cagccgccac cgggcgggaa gcctcttcac cagggcgtcg
     1141 atgctgaagt gcgcgccccg cttcaaggcc agggccgccc ccaggagagc cacccagata
     1201 aagagatacc gggccagttc ttccggccag tcaaaaggat agctgaacac ataacgcgac
     1261 accacctgga gaaaaacgac cgctgtgatg gccgccatca agacgccaca ggccagcccc
     1321 acagggtttt gatagatcac tcttacccag ctcaggattt tcatcggtcc accgttctcg
     1381 cccaaactca cctgcttcat ctccccccac ccaatcctga agcaatgaaa aaatttaggc
     1441 agcgggaatt tggagagtcc gggcatgccc ggggaggctt ggctcccctc caagggaaac
     1501 taagccaagg aagcttccga atctacttgt agctctgaat ccacttgatc ctgtccatac
     1561 caatcgcttt gccgtactgc tcgtaaacgg gttgcacgcc agcgatgaag gggtccaggt
     1621 tcggaatcac cacggttttc ttcttggtct tcagcagttc gatgatcttc tcttccgcct
     1681 cttcgttggc ttttcgttcc accggaacgc attctttgcc ggcctggatg agggcggttt
     1741 gaaactcggc ggggaggcct ttgaaaaaat tctctccgat gtacaggagt tggaccgtga
     1801 tgaggtactt gatgtgcgaa aggtagggtg ccacttcgtc gaacttcatg ctctcgtaac
     1861 tgatgacatt cccctccccg gcatccacca ccccctgctg gagggcggta tagacttccc
     1921 cgaaggggat gggcgtgggg atcagtccca gggctttcca ggtcgccacg accaccggac
     1981 tctcgatggt acggatcttc atccccttca tggaggccag gtcgctgatg ggcttccggg
     2041 catagatgct ccgcgatccc cccacccacc aggccagaat ccggaggccc tttttctggc
     2101 tggcctggtt gatttcctct ccgaattggc tgtcgaggat tttatagagg tgggggaacc
     2161 cctggaagag gaaaggaagg ttgaagattt cgatctcctt cacgaaccgg gccgccacgg
     2221 ccgaggtgga agcgcccatt tcaatggtgc cgacctgcat ggcctccacc agctcccgct
     2281 cgttgcccag ttgggagctg ggatagacct gaacttccac cttgcccttg gtcagctccg
     2341 ccaccctttt ggcgaagtgt agcgagccta tatggtaggg atgtttggta tccaggacat
     2401 gacccagctt gatcaccttt tccgcagctc cggagagccc ggggaaggaa atccccagca
     2461 ataagattcc cacagcgatg aaaatccagg ttcctttttt catttttccc ctcctgtcgg
     2521 atatcttctc tggcgaggaa ccgcaaattc taaatttttc cagccggttc tttcataagg
     2581 acccatccag ctcatctgcc accgaccttt tgcattctcg gcagcgttga gcgcccctga
     2641 ataggggttt cccgcatgaa gggcagtgat accgctcgca ttctgcccgg gcccattcca
     2701 cgctcccctt ctcatccccg tgctcggcca ctttggcacg ccagatcggg atcgtcctct
     2761 tcatcacttt cactcccgtg ccgaagccga attttttaat cagatcgcac ggccattcct
     2821 tgcattgatg acaggaataa aagcctttcc ctttcacaca gtttctgatt ctgcataatt
     2881 tgcaataacc atagatcttc ctgggcgggt cctcctgcat gcaacccagg cattcggtct
     2941 cttccggctt tgtcccgtaa agcttcccca taaccgcctt gaatttctcg ttcttatccc
     3001 gggtggcgat gtagacgccg caggcgccgc aatacaaccc acagggagac atcaaatcct
     3061 tattcctgat ttcctcctcg ctccatcctt ccatgttctt cctccgcaaa ataacacagg
     3121 gggctccgaa ccgcttagct tatcctatac atttcaggag gagctggtag acgttacccc
     3181 ccatataagc cttgatatcc tccggggaaa ggccaacctc ttgcatggca gcgactaatt
     3241 ggcttagatc acggacgtcg cggtaaccct caatgagtcg ggggatgttc ccccctccat
     3301 ccgttcccag gcccacatgg tccattccca atctcttttt catctccaga atttcctggg
     3361 cccagtccaa aaaggtcctc cgtttttggt ccgggcggtt gaacgcgaaa ggccaggtgc
     3421 agacgactcc gccggtcttg gccacccgtt ccatgtcttt ccaggagcgc tgccggtggc
     3481 accgccgcgg ttcatcgatg gggcaggggt tggtgtgcga atccagcagg gggttggggt
     3541 tcatttccac aatgcttttc aaagtggtca ggtgagcatg ggctacatcc accaccatcc
     3601 ccacttcttg catccgctcg atcacctttt gtccagctgg cgtcaggcca ttgttgtaag
     3661 gtccggggct caaagcccca tagggcacca tcacgtcacc cagttcattg tttcgataat
     3721 ggaccacggt aatgattctg acgcccatcc ggtagaattc gtcgacccgc tccggtttgc
     3781 catccaaagg gtctcctccc tcgatagcca tgatggcccc cggagggtca cctggattcc
     3841 gaatcgccgg cacatcggcg gcttttagga ccagcttcac ctttcccgcc ttcaccagat
     3901 cgtggactcg ctgtaattgg gatttggtac ttgggtaata ggcagtagcc gatgttcggt
     3961 tgaaaaattc caggtcccct accgcggcaa aggcgctggc cgccatccct gcggctctga
     4021 ttttttccag agtggagctg tcatcgatgc gacccggccg aagggtatgg aattgatccg
     4081 ggtgagcgtg gggatcgatg atttgcaggc ctcgaagaat cgcctgatcg acaactggtt
     4141 tcgctggggc ctgggcgcag gaagaaatca atccccccaa agcccatgaa acaccccaaa
     4201 gggcggaatt ctgcagaaac cgacggcggt tcatattgaa gccaccatgt ctaatctcct
     4261 ccttctaaac cgctggagaa taaaagccag ccgttccttg ggccggaccc agccggttct
     4321 atcatttacc cgatccttgc tctctttttg atttcctcca tgttcagggc gctgatccat
     4381 tggacaaggt cctgccctat cccgtgaacc ccaaaccatc ccaaagttaa atccttaatt
     4441 ttgcccataa attagcaaca aaacaggaag gaatcaaggc ctttcggaaa cctgtttttc
     4501 ttattgactc tcttttgttt ttcggatatt ttgattttga gaaattgatg ggggatcgtt
     4561 caacggtagg acaacggact ctgactccgt gaatcaaggt tcgaatcctt gtcccccagc
     4621 caaataaaaa caattggtta agccgaaagg tctaacccct ttttctttta tttctaacct
     4681 atttctaacc aaattgaaat tttccccctt tgttcacccc ccaaaaaagc atacaccgat
     4741 tgaaaaaccg atgccttttt aggggctatt gcctgcccct ccct
//
LOCUS       DASPRR010000342         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_888084, whole genome shotgun sequence.
ACCESSION   DASPRR010000342 DASPRR010000000
VERSION     DASPRR010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888084"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1141)
                     /locus_tag="VEL68_12950"
     CDS             complement(284..1141)
                     /locus_tag="VEL68_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ36918.1"
                     /translation="MNIHARGTVKRERRLEATISYALLALSAVFALFPVMWMLSTSLK
                     SEAEALSLPVRWIPQSPTFGSYVEMWTLKPFGVYFWNSIVVSGLTALLSTAVGALAGY
                     GFSRFQFRGRTSLLAFFLATQMISGVLVIGPYFQLLAAVELYNTLTGLIIAYVTICLP
                     FAAWMSKGYFDSIPKEMDEAALVDGASRFQIFCRIVMPIAVPGTVSILLFGFLLAWQD
                     LLWALCLISIDEKRTVTLGVAFTVGEFMIKWPMLTAASLIGSLPTIILYLFLQRFYVE
                     GLARGAVKG"
     gene            complement(1138..2013)
                     /locus_tag="VEL68_12955"
     CDS             complement(1138..2013)
                     /locus_tag="VEL68_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ36919.1"
                     /translation="MTSVRTWQDRHVGYLFILPATLMLVAVLVFPAFITLKLSLTPEG
                     GKGWTFTLAHYHKAFRDPVLLQTFKNTFVFVAWSLAGHLAVGLALAMALNQALPGQTF
                     FRLVALIPWTIPDVVAGIIFRWVLNPIHGCLNPLIQMVFPAFPAEFQWLSSPTLAFPS
                     LIFANVWRGYPLVMVMLLAGLMAIPRDLYEAAEVDGAGRFKRFIHVTLPGLRTIIIIA
                     LALDMVWEFRRFALVLTMTGGGPGTLTEVLSTLVYKTYFQFFTFEYASAMAILMSAVL
                     FILSLPYIFLVGRER"
     gene            complement(2088..3362)
                     /locus_tag="VEL68_12960"
     CDS             complement(2088..3362)
                     /locus_tag="VEL68_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXZ36920.1"
                     /translation="MKSFRIGLISIFVFVMIAGFSGPVNAQVKIRFQTWHWNETPWVN
                     SLNEFMKTFNQANPGIEVVRDDSRYADKESVFIAQSQAKAAADIPHFSYRAIRHLADR
                     GYLLDLTPFIEKEGGQKYLDQWDPKALEVCKYKGKIYCLPDDLNPMVLMYNTLHFKEA
                     GLDPSKPPATWEQFLDYGKKLTRASRYGVGIIAARQEGLFMRFNPWLWGAGGEYVTAD
                     EKRSALDTPEALEGFKFYVELFTRHKLVPPGVIEQGAQEVRTQLAHEKVSMIITLPAA
                     PGIVQAINPKMKVREVMALAPVPVGKKKVTSTWFSMRVISSSTKNPEAAWKVYKAWYD
                     KDSQMRNFKIAGVLSSRLDVRNSPEVKADQFARVFSAQTPYVKLEPLTSEWPKIGDAM
                     ITAIQEALTGVKTPEAALKGAHEAANRALGVQ"
     gene            3742..4446
                     /locus_tag="VEL68_12965"
     CDS             3742..4446
                     /locus_tag="VEL68_12965"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXZ36921.1"
                     /translation="MPLERKRLTDQIIDQLLAGISSGKVKPGEKLPPEPELMNQFGVG
                     RSSLREAIAALSLVGLLEVRPGHGTTVSLSTGEFLTKPLRWGMLMNWRDRIHELVEAR
                     MIIEHAMAGMAAERATKEEIAEIKYYQTQLRSSKKLGKKAVQADLAFHAALAKASHNS
                     ILARYLAELRHPMKSMMEQKASVFIDYDRVAEQHEAIVQAIEAHNPSKAQAALRDHLE
                     WAGERLKAVVLKKESK"
     gene            complement(4495..>4650)
                     /locus_tag="VEL68_12970"
     CDS             complement(4495..>4650)
                     /locus_tag="VEL68_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXZ36922.1"
                     /translation="LRQIWGPAYVEQPHLLRVNISNLRRKIEPEPTRPRYVLTELGVG
                     YRLKLKT"
BASE COUNT         1077 a         1302 c         1367 g          904 t
ORIGIN      
        1 gctccccaca cggatgacct ttttatcctg ggcttgaacc aatggggcca tccccgccca
       61 ggcaacaaga aagcaaaaaa ccgcaatcgt cgagagaacc aaaccgaatc tgatgccttt
      121 catcctttct tcctcctttc ccgtcttcat tattttatta cctccttcac taatttgccg
      181 ttgaaatcaa gggacagggc cttgccgctt ttccaatgct ttcatcccaa ccattaatcc
      241 atcaaagccc cccctctagt cctttgtttt caaccttatc cccctaaccc ttcaccgcgc
      301 cccgggccaa cccttcgaca tagaaccgct gcaggaaaag atagaggatg atcgtgggga
      361 ggctcccgat gagggaggca gcggttagca tgggccattt gatcatgaac tcccctacgg
      421 tgaaggccac ccccagggtt acggtccgct tctcgtcgat ggagatgaga cagagggccc
      481 agaggagatc ctgccaggcg aggaggaaac caaacagcag gattgaaacg gttcccggga
      541 cagcgatggg catgacgatc cggcaaaaga tctgaaagcg ggaggccccg tccacgagag
      601 cggcctcgtc catctccttc ggaatggagt cgaaataccc tttggacatc caggcggcaa
      661 agggcaggca gatggtcaca taggcgatga tgaggccggt gagggtgttg taaagctcca
      721 cggcggcgag cagctggaaa tagggtccga tgacgagcac gccggagatc atctgggtcg
      781 cgaggaagaa ggcgagaagg ctagtccggc cacggaattg gaaacgggaa aaaccgtatc
      841 ctgccagagc cccaaccgcg gtagagagga gggcggtcaa ccctgagacc acgatgctgt
      901 tccagaaata gactccgaag ggcttcagcg tccacatctc aacgtaggag ccaaaagtcg
      961 ggctttgggg gatccaccgg acgggaaggc tcagggcctc cgcctcgctc ttgagggagg
     1021 tggaaagcat ccacatcacc gggaagaggg cgaagaccgc ggacagggcg agtagagcgt
     1081 aggagatcgt ggcctccagc ctccgttccc gcttgaccgt gccccgggcg tggatattca
     1141 tcgctccctc ccgaccagga agatgtacgg caggctgagg atgaaaagca cggcgctcat
     1201 cagaatagcc atggccgaag cgtactcgaa ggtgaagaac tggaagtagg tcttgtacac
     1261 gagcgtggaa agcacttcgg tgagcgtgcc cggcccgccc cctgtcatgg tgaggaccag
     1321 ggcgaaccgt cggaactccc ataccatgtc tagggccagc gcgatgatga tgatggtcct
     1381 gagaccgggg agggttacat ggatgaaacg cttgaaccgg cccgccccgt ccacctccgc
     1441 cgcctcgtac aaatcccgcg ggatagccat gagcccggcc aggagcatga ccatcaccag
     1501 gggataaccg cgccacacat tggcaaagat caggctgggg aaggccagcg tcgggctgga
     1561 cagccactgg aactctgccg gaaaggcggg aaaaaccatc tggatgaggg ggttcaggca
     1621 cccgtggatg ggattcaata cccatcggaa gataatcccg gcgacgacgt cgggaatggt
     1681 ccaggggata agggccacca gccggaagaa tgtctgacca gggagggcct ggttcaacgc
     1741 catggccagc gccaggccca ccgccagatg accggcaagg ctccaggcaa cgaagacaaa
     1801 ggtgttcttg aacgtttgga gcaggaccgg atcacggaag gctttatgat agtgggccag
     1861 ggtgaaagtc cagccttttc ccccttcggg ggtaaggcta agcttcaagg tgatgaaagc
     1921 gggaaacacc aggaccgcta caagcatgag cgtggcaggg aggataaaaa ggtatcccac
     1981 gtggcgatcc tgccatgtcc ggacggatgt cattcaaagc ccccgcaaaa aggcagagag
     2041 tcaggggatg acccctgact cgtccaaatt tgagggatgc cgaggtccta ctgaacgccg
     2101 agcgcccgat tcgccgcttc gtgggccccc ttcagggcag cctccggggt cttcacgccg
     2161 gtaagggcct cctggatggc agtgatcatt gcatcgccga tcttgggcca ctcggaggtg
     2221 agcggctcga gcttcacgta tggggtctgg gccgagaaga cccgggcaaa ctgatcggcc
     2281 tttacctcgg gggagttccg gacgtcgaga cgggaggaga ggacgccggc gatcttaaag
     2341 ttgcgcattt ggctgtcctt atcataccac gccttgtaca ccttccaggc cgcctccggg
     2401 ttcttcgtgg aggagctgat cacccgcatg ctgaaccagg tggaggtcac cttcttctta
     2461 cccacgggaa cgggcgccag ggccatcacc tcgcgaacct tcatcttggg gttgatggcc
     2521 tggacgatcc ccggggcggc gggcaaggtg atgatcatgg agaccttctc atgggcgagc
     2581 tgggtccgga cctcctgcgc cccctgctca atgaccccgg gcgggactag tttgtgcctg
     2641 gtgaacaact ctacatagaa cttgaagccc tccagggcct cgggggtgtc gagggctgag
     2701 cgcttctcat cggcggtcac atattcccca ccggctcccc agagccaggg gttgaagcgc
     2761 atgaagaggc cctcctggcg ggccgcgatg atccccacgc cgtagcggct cgcgcgggtc
     2821 aacttttttc catagtccag gaactgttcc caggtggccg ggggcttgga ggggtccaat
     2881 ccggcctcct tgaagtggag ggtgttgtac atcaagacca tggggttcaa atcatccggc
     2941 aggcagtaga tcttcccttt gtatttgcag acctccagcg cctttgggtc ccactggtcc
     3001 aggtatttct ggccgccttc cttttcgatg aagggtgtca ggtccaagag gtacccccgg
     3061 tcagccaggt ggcggatggc ccggtagctg aaatggggga tatccgccgc tgctttggcc
     3121 tgggactggg cgatgaatac cgattctttg tcagcatagc ggctgtcgtc cctcacaacc
     3181 tctatgccgg ggttcgcctg gttgaaggtc ttcatgaatt cgttcaagga attcacccag
     3241 ggcgtctcgt tccagtgcca ggtctggaac ctgattttta cctgggcgtt cactggccct
     3301 gaaaacccag caatcatcac gaaaacaaaa atcgaaataa gaccgattct aaacgatttc
     3361 attttcatgc cctcctttgt atcgttattc ccaatttcga gttgcgggtt tccaattcag
     3421 aagcctaact tggcactccc tcgctccaaa ccccgaaaac cacctgcgcg cgactctaat
     3481 tttatgaaga actttagaac ctgccccccg ccacttcttc cttctatctt ctatgcctgc
     3541 gggattacct tcttttagcc ggattagggg gcggtgtcaa ggaaaaaagt tgatcttctg
     3601 atcatcagat gataatatga attccaggtg ccgaccggca aagagcagga agctcccttg
     3661 accgaacaaa ttgcataggc taggccattt caggggctgc ttaaggaccc caaagaggaa
     3721 tcagaaatga gaacttccgc catgcctctt gaaagaaaac ggttgaccga tcaaatcata
     3781 gaccagcttc ttgcgggcat atccagcgga aaggtgaaac ctggagaaaa acttccgccg
     3841 gaaccggaac tcatgaatca gttcggggta gggaggagtt ccctccggga agccattgcc
     3901 gccctgtccc tggtgggcct gctggaagtt agacccgggc atgggacgac cgtcagcctc
     3961 tccaccggag aatttctgac caaaccgctc cggtggggca tgctgatgaa ctggagggac
     4021 aggatccatg aactggtgga ggcccggatg atcattgaac atgccatggc cggaatggcc
     4081 gcggagagag ccacgaagga ggaaatcgcc gagatcaagt actaccaaac ccagctgagg
     4141 tcgtcgaaga aattggggaa aaaggcggtg caagcggact tggctttcca tgccgccttg
     4201 gccaaagcca gtcacaacag tatcttagcc cgctacctgg cggaacttcg ccatcccatg
     4261 aaaagcatga tggagcagaa agcctcggta ttcattgatt atgaccgcgt cgccgaacag
     4321 catgaggcca tcgtccaagc catcgaagca cacaatccca gtaaagccca agccgccctc
     4381 agggatcatc tggaatgggc cggagaaaga ctcaaggccg tcgtcctgaa aaaggaatcg
     4441 aagtaatctc gtaaatcgga tcgcgggaag ccgcttctct ttcaaattta aggtctaggt
     4501 ctttaattta agccggtatc ctacgccgag ctccgtcagg acataccggg ggcgggtagg
     4561 ttccggctcg atcttgcggc ggaggttgct gatgttgacc cggagcaaat ggggctgttc
     4621 cacataggcg gggccccaga tctgccggag
//
LOCUS       DASPRR010000343         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_397990, whole genome shotgun sequence.
ACCESSION   DASPRR010000343 DASPRR010000000
VERSION     DASPRR010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397990"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VEL68_12975"
     CDS             <1..884
                     /locus_tag="VEL68_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HXZ36923.1"
                     /translation="FLAFIGLNETGIVVPGVPGAPVSLGKLSQPSVLLALLGFVIISW
                     LTIRKVTGAIIIGILVTTLISCWWGISAVPKAVMSSPPGLGPIFLELNLSEALSLKSL
                     PVVVIIFVMAFVDTVGTLIGLSARANLLDQKGNLPEIEKPMLADALANLIAPVLGTTT
                     TGAYIESAAGISEGGRTGFTALVVAVLFLLALFFAPLFTAVPPHAYGTALIVIGIFMI
                     SPITKINFQDYTELIPAFLTITLIIFTFNIGVGMTAGLLSYPVFKILAGRPREVPLGM
                     WIFAGFSLLFYIFYPYK"
     gene            1180..1944
                     /gene="mtnP"
                     /locus_tag="VEL68_12980"
     CDS             1180..1944
                     /gene="mtnP"
                     /locus_tag="VEL68_12980"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXZ36924.1"
                     /translation="MVKVGIIGGSGLDNPDILKQAAEIEVETPYGSPTSLLTRGKIEG
                     VEVAIIARHGKNHSIFPSKVNFRANIWALKEQGCTHVLATTAVGSLREEIKPGHLVFP
                     NQFIDHTKKRELTFFDEDVVVHTPMAEPFCSRLIELCSLSAGRLKIPFHQNKTVITIE
                     GPRFSTRAESIMFRGWMADVINMSTVPEVNLAREKKMHYASVAMSTDYDCWRAGEESV
                     TWETIVQMMQKNAANVIKLFLEVIPQIRAYDDVCIK"
     gene            1964..2497
                     /locus_tag="VEL68_12985"
     CDS             1964..2497
                     /locus_tag="VEL68_12985"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXZ36925.1"
                     /translation="MTIKSRIRTVPDYPKKGVMFRDITTLIKDPVGFRLVIDNFTQRY
                     IQGDIQVDLIVGIEARGFIIGGALSYTLGKGFVPIRKRGKLPAEVVTHEYDLEYGKDT
                     VEMHRDAIVKGAKILVVDDLLATGGTALAAASLVEKLGGSVSELAFIVNLPDLGGCEK
                     LRSRGYNVFCLTDFEGD"
     gene            2633..2866
                     /locus_tag="VEL68_12990"
     CDS             2633..2866
                     /locus_tag="VEL68_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36926.1"
                     /translation="MGSYEWLEYEKGKALLAFLEEVKRSVQNLQPIGSLAVGVGKAIG
                     DTIGSKARELEIPPEEAVEYFFSTAYSRNRRFP"
     gene            2884..>3874
                     /locus_tag="VEL68_12995"
     CDS             2884..>3874
                     /locus_tag="VEL68_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HXZ36927.1"
                     /translation="MGSKSMALKRPPFYDSLRAFLFVLKKERFFLILGITIVILFCGA
                     AALYLFEHQVNKTVASLGDAIYWAVISMTTTGYGDIVPSTTGGRLVAVFVVLSGLFLL
                     SMVTATVASVFVEKKIREGKGLETIKTKNHILLCGWNSNAEEVIEGLLRSMAVKEVHL
                     VLVNELSEGEVESLKYKYRDQDFRFLRGDFTREDVLSRANILQARFAILLADTSGQRS
                     LEKADERTILATLAIKSMAPEVKTCAELLNPENKQHLLRANVDEIVLRGEHIGSLLAG
                     ATVSPGIPRVISSLLSPEEENNLWKVEVPERFVGKPVAELSAHLKEKFQALLIA"
BASE COUNT          815 a         1135 c         1085 g          839 t
ORIGIN      
        1 tgttcctggc atttatcgga ttgaatgaaa cgggcattgt cgtgcctggc gtgcccgggg
       61 cacccgtgag cctgggcaag ctttcccagc cctccgtcct cctggccctg ctcggctttg
      121 tcatcatctc ctggctcacc atcaggaagg tgacgggggc catcatcatc ggcattctcg
      181 tgaccaccct gatctcttgc tggtggggga tctcggccgt gcccaaagcg gtcatgagtt
      241 cgccgcccgg cctcggcccg atctttctgg agctgaacct ctccgaggcg ctaagcctca
      301 agagtcttcc ggtcgtggtc atcattttcg tcatggcctt cgtggatacg gtggggaccc
      361 tcatcggcct ctcagcgcgg gccaacctgc tcgaccagaa gggcaacctg ccggagatcg
      421 aaaagccgat gctggccgac gcgctcgcca acttgatcgc ccccgtcctc ggaaccacca
      481 cgaccggcgc ctatattgag tcggcggcgg gcatttccga ggggggacgg accggtttta
      541 cggcgctcgt ggtcgccgtg ctcttcctgc tggccctgtt ctttgcgccc cttttcacgg
      601 cagtgccgcc ccatgcttac gggacggccc ttatcgtgat cggcatcttc atgatcagcc
      661 cgatcaccaa aatcaacttc caggattata cggaactcat ccccgctttc ctgaccatca
      721 ccctgatcat cttcactttc aacatcggcg tggggatgac cgcgggcctg ctttcctacc
      781 cggtctttaa gattttggcc ggccggcccc gggaagtccc gctgggcatg tggatattcg
      841 cgggcttttc gctgctgttc tacatctttt atccttacaa gtaaaactcc gggaaccccc
      901 tcagccactc atcccccgcg ggcaaacagg tgcaccgctg ttgggtcttc ccacaggtcc
      961 gtctctgggc ctcaggcttt ttattcggca tccttcaacg cttcggaaaa ggccttacca
     1021 aaaatccctc ccccctccct ttttcaaagg gaggagagtt cctttcattt tccttgacac
     1081 ccttccccgc ctttccatac aattgcagga tggaagatcg ggcattttcc gatttacctg
     1141 catagtaagg aaacgctctc gattcggaag ggatcatgaa tggttaaggt gggaatcatc
     1201 ggagggtccg ggctcgacaa tcccgacatc ctgaaacagg cggccgaaat agaagttgaa
     1261 actccttatg gcagccctac ctcccttctt acccgcggga agatcgaagg ggtcgaagtg
     1321 gcgatcatcg cccgacacgg caagaatcac tccatattcc cttccaaggt gaatttccgg
     1381 gcgaatatct gggcgctgaa ggagcagggc tgcacccacg tcctggccac caccgccgtc
     1441 ggttccctga gggaagagat aaaaccgggc cacttggtat tccccaatca gttcatcgac
     1501 cacaccaaga aaagggaatt gaccttcttt gatgaggacg ttgtcgtcca cactcccatg
     1561 gccgagcctt tttgctcccg cttgatcgaa ttgtgctccc tgtccgcggg gcgcctgaaa
     1621 atccccttcc accagaataa gacggtcatt accatcgaag gcccccggtt ttccaccagg
     1681 gcggaaagca tcatgttccg gggatggatg gccgatgtca tcaacatgag cacagtgccg
     1741 gaggtgaacc tggcccggga aaagaagatg cattacgcgt ccgtggccat gtccacggac
     1801 tatgactgct ggcgggcggg ggaggaatcg gtgacctggg agaccatcgt gcagatgatg
     1861 caaaagaatg ccgctaatgt catcaagctc ttcctggagg tcatccccca aatccgcgcc
     1921 tatgacgatg tgtgcataaa ataaccccaa gggagcggcg agcatgacta tcaagtcccg
     1981 aatccggacc gtccccgatt atcccaaaaa gggagtcatg ttccgggaca tcaccacgct
     2041 gatcaaagac ccggtagggt ttcgcctggt gatcgataat ttcactcaga gatatatcca
     2101 gggcgatatt caggtggact tgatcgtggg catcgaggca cggggtttta tcatcggcgg
     2161 ggccttgtcc tatacgctag ggaagggttt cgtccccatc cgcaaacggg gcaagctccc
     2221 ggccgaagtg gtgacgcatg aatacgacct ggaatacggc aaagacacgg tggaaatgca
     2281 tagagatgcg atcgtaaaag gagccaaaat cctcgtggtc gatgacctgc tggcgaccgg
     2341 cgggaccgcc cttgccgctg cttccctggt ggaaaaactg ggcggttcgg taagcgagtt
     2401 ggcctttatc gtcaacctcc cggacctcgg cgggtgcgag aaattaagaa gcaggggata
     2461 taatgttttt tgcctgacgg actttgaagg agattgagat acaccctgcc tctccggaga
     2521 ccatccaggg gcttgaggat tccccgggct acaacccgcg cccggcaccc ggcaatggaa
     2581 atgaatgtgg ggctcctgcc gggaaccatt cccggcatcg gaggtgcaag aaatgggcag
     2641 ctacgagtgg ttggaatatg aaaagggaaa agcgcttctg gcctttttgg aggaggtgaa
     2701 aaggtccgtc cagaaccttc agcccatcgg ttcattggcc gtgggagtcg gcaaggccat
     2761 cggggatacc atcggatcga aagccaggga attggaaatt ccccctgaag aggccgttga
     2821 atatttcttt tccacggcgt attcccggaa ccgccgtttc ccctaggggg aagaaaggct
     2881 aaaatggggt ccaaatccat ggccctgaaa agacccccat tttacgactc cctccgagct
     2941 ttcctcttcg tcctgaagaa ggagagattt ttcctcatcc tgggaatcac catagtcatc
     3001 cttttttgcg gggccgctgc tctctacctg ttcgagcatc aagtcaataa aaccgttgcc
     3061 agcctggggg atgctattta ctgggcggtc atctccatga cgaccaccgg gtacggggat
     3121 attgttccct ccaccaccgg gggccggctg gtcgctgtct tcgtcgtcct gtcggggtta
     3181 tttttgctct ccatggtcac ggccacggtt gcttccgtgt tcgtggagaa aaaaatccgt
     3241 gagggaaaag gattggaaac gatcaaaacc aagaatcaca tcctgctctg cggctggaac
     3301 agcaatgccg aggaggtcat cgagggattg ctccggtcca tggccgtgaa agaggtacac
     3361 ctggtcctgg tcaacgagct ctccgagggc gaggtggagt ccctgaaata caagtaccgg
     3421 gatcaagact tccggttcct ccggggagac tttacccggg aggatgtgct gtcccgggcg
     3481 aatatccttc aggctcgctt tgccatcctc ctggcggaca cctcggggca gcgctccctg
     3541 gagaaagccg acgaacggac gatcctggcc accctggcca ttaagtccat ggccccggag
     3601 gtgaagacct gcgccgagct tctcaacccg gaaaacaagc agcacctgct gcgggcgaat
     3661 gtggacgaga tcgtccttcg gggggaacat atcggaagcc tcctggccgg ggccacggtc
     3721 tccccgggga tcccccgggt catctccagc ctcctctctc cagaggagga gaacaacctc
     3781 tggaaagtgg aggtcccgga gcgcttcgtg gggaaacccg tcgccgagtt atcggcccac
     3841 ctgaaggaaa aattccaggc tctgctcatt gctc
//
LOCUS       DASPRR010000344         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_732143, whole genome shotgun sequence.
ACCESSION   DASPRR010000344 DASPRR010000000
VERSION     DASPRR010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732143"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VEL68_13000"
     CDS             complement(<1..328)
                     /locus_tag="VEL68_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXZ36928.1"
                     /translation="MAEEFPFVFPKLKVNKSFYLVIVTRGHAYDQEVLEWALGQEVKY
                     IGMIGSRKKIQTVYDNLQAKGITREKLEAVHGPIGSSIGARTPEEIAVSIVAEMIQVR
                     RQKKGEK"
     gene            complement(450..923)
                     /locus_tag="VEL68_13005"
     CDS             complement(450..923)
                     /locus_tag="VEL68_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ36929.1"
                     /translation="MKVQFKLNGRAIAEEVEPCETLLDFLRERLKVISVKKGCDEGNC
                     GACTILLNGKPVRSCLLLAVQMRESDEVTTVEGLAKPGGVSPIQEAFLEKYGFQCGFC
                     TPGMILTTKALLDANPKPTVEEIKDQFEGNLCRCTGYQQIIESVLRAAELRARRS"
     gene            complement(907..1797)
                     /locus_tag="VEL68_13010"
     CDS             complement(907..1797)
                     /locus_tag="VEL68_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXZ36930.1"
                     /translation="MEAEVRPLPKMDYVAPSSLKEALRLLKKYRGKAKVLAGGTDLIP
                     KLKKRMVKAEMLIDLNRIPGLSGIRLQKDGLHIGGLTRLAEIKESRLVREKAPVLVQA
                     IGVMASPPIRNRGTLGGNLCTASPAADTAPPLLVLNASVRLQNTNGKRVVPIFEFFKG
                     PQDTFRKPDEMLTEVIVPVQKGRSAFLKLGRRKAFTLSVVSAAAFARVRGGKFEDVRL
                     ALGAVAPTPIRSRKVEEALRGKEVNEENIARAAELVKGEVRPIGDVRASEEYRREMSS
                     VLTRRVLAQVNERGYNESAI"
     gene            complement(1781..>2528)
                     /locus_tag="VEL68_13015"
     CDS             complement(1781..>2528)
                     /locus_tag="VEL68_13015"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXZ36931.1"
                     /translation="TFPHFGDEPVHSLAALKLRKRMNRLVAEMWECSEDDVAFENGET
                     YNLKSPKQRIGFKDLAWEMYMKGIPPAEYGFIKARRGYPDPETGQGDPYAAYTFGCTI
                     AEVEVDLETGQVSVLRLIPGVAAGKIIQPAVAKGQVYGCGMLGLGYATTERVLRKDGR
                     MINTSYTDYLIPTIKDMPEMADLVVVEDEYKFSGFGAKGVGEIAFIATPLAIANALNQ
                     ALGIRFYDMPLDMEKVYFAIREKKSHGSRS"
BASE COUNT          506 a          787 c          626 g          609 t
ORIGIN      
        1 acttttcccc tttcttctgg cggcggacct gaatcatctc cgctacgatg ctcacggcaa
       61 tctcctcggg cgttcgggcc ccaatgctcg atccaatagg gccatggaca gcctccagtt
      121 tttcccgggt aatcccttta gcctgtagat tgtcataaac cgtctgaatc ttcttccggc
      181 ttccgatcat gccgatgtat ttaacctcct gtcccagggc ccactccaaa acttcttggt
      241 cataagcatg gccgcgggtt acgatgacca agtagaaaga tttgttcacc ttcaatttcg
      301 gaaagacaaa agggaattct tcggcaatga cctcatcggc ttcggggaag cgctctttga
      361 aaactgcggc cggtcctggg cctcaccctt tctctccccc tctaggggga agagctggga
      421 gggagtgatc aggaaaaaaa gaaataagac tatgaacgcc gggccctcag ctctgccgcc
      481 cgcagcaccg attcgatgat ttgttgataa ccggtgcatc ggcatagatt cccttcgaac
      541 tgatccttga tctcttccac cgtcggcttg ggattcgcat ccaataaagc tttggtagtg
      601 aggatcatgc cgggggtgca aaaaccgcac tggaacccgt acttctccag gaaggcctcc
      661 tggatcggac ttaccccgcc cggtttggcc aggccttcaa cggtagtcac ttcatcgctt
      721 tcgcgcattt gtacggcaag taggaggcag gaccgtaccg gtttgccgtt caggagaatc
      781 gtgcaggctc cgcagttccc ttcgtcacat ccttttttca cactgatgac cttgagtctt
      841 tctcgcaaaa aatccagcaa ggtttcgcac ggctcgactt cttcggctat cgcccggccg
      901 ttcaatttaa attgcacttt cattatatcc cctctcgttc acctgcgcca gaacccttcg
      961 ggtaaggact gaggacattt cccttcgata ctcttccgag gcccgcacat cgccgatcgg
     1021 cctcacttcc cccttgacga gttccgccgc gcgggcgatg ttctcttcgt tcacttcctt
     1081 cccccgcaaa gcctcttcca ccttccggct gcgaatggga gttggagcca ccgctcccag
     1141 ggccagccgc acgtcctcga atttcccacc ccgcacccgg gcgaaggccg ctgcggaaac
     1201 caccgatagg gtgaaggctt tccgtcgccc cagcttcaaa aaagccgaac ggcctttctg
     1261 gaccggaact atcacctcgg tgagcatttc atccggcttc cggaacgtat cctggggccc
     1321 cttgaaaaac tcgaatatcg ggacgactct ctttccgttc gtattctgca atcgaaccga
     1381 ggcattaagg acgaggaggg gaggcgcggt gtctgcggcc ggagaggcag tgcataaatt
     1441 cccccccagg gtgccccggt tccggattgg aggagaggcc atgactccga tcgcctggac
     1501 gaggaccggc gccttctccc tgaccaagcg cgattctttg atctcagcta ggcgcgtcag
     1561 ccccccgatg tgaaggccat ccttctgaag tctgatcccc gacaggccgg ggatgcgatt
     1621 caggtcgatg agcatttcgg ccttgaccat cctcttcttc agcttgggaa tcaggtcggt
     1681 ccctccggcc aggaccttcg ctttacccct gtatttcttc aacagtcgga gggcttcctt
     1741 cagggaagaa ggggccacat aatccatttt cggcaaaggc ctaacttctg cttccatggc
     1801 tctttttctc ccttatggcg aaatagactt tttccatatc cagcggcata tcatagaacc
     1861 ggatgcccaa ggcctggttc agagcgttgg cgatggccag cggggtggcg atgaaggcaa
     1921 tctcccccac ccccttggcc ccgaaacccg aaaatttgta ttcgtcttcc accaccacca
     1981 ggtcggccat ctcgggcata tccttaatgg tgggaatcaa gtaatcggta taggaagtgt
     2041 ttatcatccg gccatctttc cgtaataccc tctctgtcgt ggcatatccc agccctaaca
     2101 tcccgcaccc gtaaacctgg cccttggcca ctgccggctg gatgatcttt ccggcggcca
     2161 cgcccggaat tagccggaga acactcacct gcccggtctc caggtctact tccacctcgg
     2221 caattgtgca tccaaaggta taagcggcgt aggggtctcc ctgccccgtt tcggggtcag
     2281 gatatcccct cctggctttg atgaatccat actctgccgg gggaatcccc ttcatgtaca
     2341 tctcccaggc caaatcctta aaaccgatcc tctgtttggg actcttgagg ttgtaggtct
     2401 ctccattctc gaaggccacg tcgtcttccg aacattccca catttcggcg acgagccggt
     2461 tcattctctt gcggagcttc agcgccgcaa gagaatgaac cggctcgtcg ccgaaatgtg
     2521 ggaatgtt
//
LOCUS       DASPRR010000345         6393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1206936, whole genome shotgun sequence.
ACCESSION   DASPRR010000345 DASPRR010000000
VERSION     DASPRR010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206936"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1445)
                     /locus_tag="VEL68_13020"
     CDS             complement(216..1445)
                     /locus_tag="VEL68_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36932.1"
                     /translation="MVKSKVRKVGKGLIILVALLAFCTAPAFAQDKVRIGVLLPFSGP
                     LAYEGNEVFKGFEVARVEQNEKGGLLGKQIEFLKGDAVDPKAAVSEAERLISVEKVSV
                     ILGTFSSPRSYAASTIAEKNKVVYWETGSVGDDITKRGYKYIFRICPNAEDLAKAASY
                     FGVVVAPRMLKIAAKDLKVAVIGENSLYGTSVSKTAKGMVEAEGAQLVAYEFYDANTK
                     DLSSLILRLKAKKPDVLIPTCYVNDAILLSRQSKQMDFNVKVFVGTGVGHNTKALADA
                     IGDDVNGILCSGWPSTTLNKKFAWGLEAFMKTYEKVWKEPIGSPHPFGNYTGAWVLWD
                     TIKRANSMDAEAIRKAAEATDIPKGKTPIGWGVKFVPPDQINAGQNMRALWFVEQWRD
                     RKLHCAWPDDAKEAGYR"
     gene            complement(1535..2476)
                     /locus_tag="VEL68_13025"
     CDS             complement(1535..2476)
                     /locus_tag="VEL68_13025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ36933.1"
                     /translation="MIIDSHAHLWIEDWLPEAFWNGMAQRVCTVRKRTRGEDLTVETV
                     KKTLFPAYWDQDGRVLIREMDEAGIDKTIIVPLDLGIELGEAKVSVEEINESYAAVAK
                     RYSGRIFAYAGVDPRRPNAAEVLERGVKHWGMKGLKLDPAAGFYPNEEFCYPLYRKAS
                     DLGVPVLFHTGSTIPPFRNKYTQPIYLDDVSLDFPDMTLIAAHMGFGWWQELASMIAK
                     RTNLITDISGWQQNAIRHYPTFVKTLREMMHLVGPDNILFATDGPSFRLYNLPNKQWA
                     KMIRNLPREAPEGITFTEGEIELLLGKTAQKHLKLEG"
     gene            complement(2493..3539)
                     /locus_tag="VEL68_13030"
     CDS             complement(2493..3539)
                     /locus_tag="VEL68_13030"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ36934.1"
                     /translation="MKAIVKSSPSPGIEVREVEPPRPQPGEVLVRVEAVGICGSDIHI
                     YEWTPGYDFLSKYFPLTLGHEFAGQIMESRGGSFRSGEKVTSETGRICGKCFFCKQGK
                     GILCALRTSFGRIGLERNGAMAEYVVVHEDCLHRIPPGVTQEEAAMTEPAAVALGAVE
                     LAHFYPGDVVVILGPGPIGLLILQMCKALGAGKVGVFGLEADRPRLRVAETLGADFTL
                     IGENEDSLKKIMDLTEGLGAGVVFEVSGSPAAAVPGLQMVRKAGEMILVGIYPGPIPV
                     DATHQMVRQMKTIKGSYGGASLDWDRVLKLVAARKINLAPLISRVLPLEQAKEGFEAV
                     LNRNALKVILKTNQ"
     gene            complement(3539..4561)
                     /locus_tag="VEL68_13035"
     CDS             complement(3539..4561)
                     /locus_tag="VEL68_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXZ36935.1"
                     /translation="MAPTIKDIARQAGVSIATVSRTINGEDGVGPETRERIQAIARRL
                     NYFPNLSARSLVAQKPHALGIVIPRTSEFAFSNPYYAEILKGIGEKTNESGQYLVLSF
                     LREEDYARMYHSCLAAGIIVLANRIEDPGVAHARKSRIPMVLIPGDPLKPDVPSVDGD
                     NYDGVRQAIRHLAQLGHRDIALFRGPMNSKYSIERRDAFQQAMKKHSLPFREDWVIEY
                     DFTQEGGYVEMKKLLALENRPTALLLMNDFSALGVLRAAKDSGVRVPEEVSLIGFGDV
                     PSASMTDPPLTTLREPFQTMGYRAADMLLKMIQGRRFTPKHIVLPVELVIRKSSAPPP
                     KKRRKV"
     gene            complement(4570..5547)
                     /locus_tag="VEL68_13040"
     CDS             complement(4570..5547)
                     /locus_tag="VEL68_13040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ36936.1"
                     /translation="MKRRFLLAFLASVTLTGVGSQVWAEYPERPITLIANYSAGGGAD
                     LSARALAKKAEKALGQPVAVVNRAGAGGTVGMAAIAASKPDGYTIGVTTYGPLTFAPH
                     MNDLPYDPLKDFEYIMGYGRYMYGPVARSDSQFKTLKDLVQYAKANPGKVKYSLIGLP
                     TPNNFGMVYLAKAEGIKWEPVVFKETPAAVAACLGGHVDIVSQNPGDVVSYIKAGRFR
                     LLASFSDIRWPWVPDVPTAKELGYKFDVNSWLALGTPKGVPKPAMDKLREAFKKAMDD
                     PEFIEIMNKIYIPPAFKTPEEYKNLVEVGYKESEAMIRELGLHKSQQKK"
     gene            complement(5689..>6393)
                     /locus_tag="VEL68_13045"
     CDS             complement(5689..>6393)
                     /locus_tag="VEL68_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ36937.1"
                     /translation="ILPGGGALISSFVSYALEKKVSRTPERFGQGAIEGVAAPETANN
                     AATGGAFIPLLTLGIPANVVMAMLFGALLIHGMQPGPMLLQSHPDLFWGVVTSMYVGN
                     GMLLLLNLPLIGLWVQVLKVPYRILFPLILLFCLIGAYSINYSVMDVLIMIVFGVFGY
                     LMKKFEYEGAPLVLAYVLGPLLEMSLRQSLTMSDGHFSIFFTRPISAVGIGVALLLVI
                     SPFLPWLGKKRPAMAE"
BASE COUNT         1355 a         1860 c         1735 g         1443 t
ORIGIN      
        1 ccagcaggag gccattaatg gtcagttgaa gaatggtttc caagatcgaa tgctcctaaa
       61 aggctggata gaaagatatc gagaaagata gaccttgccc taaccaagcg actgacaatg
      121 ccagcgtgga ggcaattcat gaattgcccc aacgccatcc taagaattcc cagtctactt
      181 tttctttcgc tcttcccagg tggggtaagg caaaatcacc ggtacccggc ctctttggca
      241 tcgtcaggcc aggcgcaatg cagctttcta tccctccatt gttctacgaa ccagagggcg
      301 cgcatgttct gcccggcgtt tatctgatcg gggggaacga atttcacccc ccagccgatg
      361 ggggtctttc ccttggggat gtccgtggct tccgcggcct tgcggatcgc ttccgcatcc
      421 atagagttcg cccttttgat cgtatcccag agaacccagg ccccggtgta gttgccaaaa
      481 ggatgggggg acccgatggg ctctttccac actttttcgt aggtcttcat gaaggcctcc
      541 aggccccagg caaatttctt gttgagcgtg gtgctcggcc atcccgagca gagaatcccg
      601 ttcacatcat cgccaatggc atcggccagg gctttggtgt tatgccccac cccagtcccc
      661 acgaacacct tcacgttgaa atccatctgc ttggactggc gggaaaggag aatagcgtcg
      721 ttgacgtaac aggtggggat gaggacatcg ggcttcttgg ccttaagacg caggatgagg
      781 gaagagaggt ctttggtatt ggcatcgtag aattcatagg ccaccagctg ggctccttcc
      841 gcttccacca tccccttggc ggttttgcta accgaggtcc cgtagaggga attttccccg
      901 atcaccgcaa ccttcaaatc tttggccgcg attttcagca ttctcggagc cactaccacg
      961 ccgaaataag aggctgcctt ggctaggtct tcggcattgg ggcaaatgcg gaagatatac
     1021 ttgtaacccc gcttggttat atcgtcgccc acggaccccg tttcccagta gacgacttta
     1081 ttcttctcgg cgatggtcga ggcagcatag ctcctaggac tggaaaaagt ccccaggatc
     1141 acgctcacct tctccaccga aatcaacctc tccgcctcgc tcaccgcagc cttggggtcc
     1201 accgcatccc ccttcaaaaa ttcaatctgc ttgccgagga gacctccttt ttcattttgt
     1261 tctacccggg ccacctcgaa tcctttgaag acctcgttgc cttcataggc caggggaccg
     1321 ctgaagggca ggagcactcc gattctgacc ttgtcctggg caaaagccgg cgccgtgcag
     1381 aaagctaaaa gagcaaccag aatgatcaac cctttaccca ctttccttac cttcgactta
     1441 accatggctt ttctcctttc ctcgggatta tggaagcgga tagaaaacat ccctttcctc
     1501 ttgttattcc tttctgggat aaaagcgaga atcttcagcc ctctaattta aggtgcttct
     1561 gggcggtttt tcccagaagg agttcgatct ccccctccgt aaaagtaatc ccctccgggg
     1621 cctcccgggg gaggttgcga atcatcttgg cccattgttt gttaggcaga ttatagagac
     1681 ggaatgaagg gccgtccgtg gcaaagagta tgttatccgg cccgaccagg tgcatcatct
     1741 cccggagggt cttgacaaac gtcgggtaat ggcggatggc attctgttgc cacccggaga
     1801 tgtcggtaat gaggttagtc cgcttggcga tcatggaggc cagttcctgc caccagccga
     1861 aacccatatg ggcggcgatg agggtcatat ccgggaaatc caacgagaca tcatccaaat
     1921 agatgggttg ggtatatttg ttccggaagg ggggaatggt cgagccggtg tgaaagagaa
     1981 ccgggactcc caaatccgaa gccttccgat aaagaggata acagaattcc tcattgggat
     2041 aaaaccctgc cgcagggtcg agcttcagcc ctttcattcc ccagtgtttg accccccgtt
     2101 cgaggacctc cgcggcattc gggcgacggg gatcgacccc tgcataggcg aagattcggc
     2161 cggaatatcg cttggccacg gcggcgtaac tttcattgat ttcttcaacc gagaccttcg
     2221 cctctcccag ctcgattccc aggtccaaag gaacaataat cgtcttgtcg attccggctt
     2281 catccatctc tcgaatcagg acgcggccat cctggtccca gtaagccgga aacaaggttt
     2341 tcttcacggt ttccacggtg aggtcttccc cccgggttcg tttgcgcacc gtacagaccc
     2401 tctgggccat gccgttccag aaggcttcgg gcaaccagtc ttcgatccat agatgggcgt
     2461 gggaatcgat aatcattctg ccctccctcc ttttattggt tggtcttcaa aatgactttc
     2521 aaggcgtttc tgttcaggac ggcttcaaag ccttcctttg cttgctccag gggaagcacc
     2581 cgcgaaatca gaggagccag gttgatcttc cgggcggcca ccaacttcaa cactcggtcc
     2641 cagtccaggg aagctccgcc gtagcttccc ttgatggtct tcatctgccg gaccatttgg
     2701 tgggtggcat ccacgggaat gggtccggga tagatcccca cgaggatcat ctctccggct
     2761 ttgcgcacca tctgaagtcc ggggactgca gcggcgggcg agccgcttac ctcaaatacc
     2821 acgccggccc ccagcccttc ggtcaggtcc atgatctttt ttagggaatc ctcgttttcg
     2881 ccgatcaggg tgaaatcggc gcccaatgtt tcagccaccc ggagccttgg gcgatccgct
     2941 tccaggccga agacccccac cttccccgcc cccagggctt tacacatctg gaggatcaga
     3001 aggccgatgg ggccgggtcc gaggatcacg acaacatcgc ccgggtagaa atgggcaagt
     3061 tccaccgccc ccaaggccac ggccgcaggt tcggtcatgg ccgcttcttc ctgggtcacc
     3121 ccgggcggaa tccgatgcag gcaatcttcg tgcaccacca catactcggc catggctccg
     3181 ttccgctcca gaccgattcg gccgaaggag gttcgcaaag cgcaaaggat ccccttcccc
     3241 tgcttgcaga aaaaacactt cccgcagatt cttcccgtct cgctggtcac tttttctccg
     3301 ctccggaagg aaccgcctcg ggattccata atctgtccgg cgaattcatg gcccagggtc
     3361 aaggggaaat atttggacag gaaatcataa ccgggggtcc attcgtaaat gtggatgtcg
     3421 ctcccgcaga ttcccacggc ctcgacccgg accaatactt cccccggctg gggccggggg
     3481 ggctctacct cccggacctc gattcccgga gaaggggaag atttaacgat cgctttcatc
     3541 agacctttct cctctttttc ggcggggggg cgctggattt tcgaatcaca agttccacgg
     3601 gaaggacaat gtgcttgggc gtaaatctcc tgccctggat catcttgaga agcatgtctg
     3661 cggcccgata acccatcgtc tgaaaaggct cacggagagt ggttaaaggc gggtcggtca
     3721 tggatgcgga gggaacgtct ccgaacccga tcagggaaac ctcctccgga acgcgcaccc
     3781 ccgaatcctt cgccgcacgc agcactccca gggcactgaa gtcattcatg agcaggaggg
     3841 ccgtcgggcg attttcaagg gccagaagct tcttcatttc cacgtaaccc ccctcttggg
     3901 tgaaatcgta ttcgatcacc caatcttccc ggaagggaag ggagtgcttt ttcatcgcct
     3961 gttgaaatgc atcccgccgt tcgatgctgt atttggaatt catcggcccc cggaagaggg
     4021 caatgtcccg atgtcccaac tgggcgagat gacggatggc ctgccgaacg ccatcgtagt
     4081 tatctccgtc gacgcttgga acatctggct tcagagggtc tccggggatc agcaccatcg
     4141 ggattctgct cttacgagcg tgggccacgc ccgggtcttc gatccggttg gccaggacga
     4201 taattcctgc cgccaagcag gaatgataca tccgggcata atcttcctcc cgcaggaagg
     4261 aaaggacgag atattggccg ctttcgttgg ttttttcccc gatccccttg aggatctccg
     4321 cgtagtatgg attggaaaag gcgaactccg acgtgcgggg gatcacgatc cccagggcat
     4381 ggggcttttg ggccactagg ctgcgggcgg agaggtttgg aaaatagttc aggcgccggg
     4441 caatggcctg gatcctttcc cgagtctccg ggcccacccc gtcttcgccg ttgatggtac
     4501 gggaaacggt ggcgatggag acccccgcct gccgggcgat atccttaatg gttggcgcca
     4561 tacagccttt tactttttct gctgggattt atgcaggccc agttcccgga tcatggcttc
     4621 gctttctttg tatccaactt ccaccaggtt tttgtattcc tccggggttt tgaaagctgg
     4681 cggaatatag atcttgttca tgatctcgat gaattccgga tcatccatgg ccttcttaaa
     4741 ggcttcccgc agcttgtcca tggcgggttt gggaaccccc ttgggcgtgc ccagcgcgag
     4801 ccaggagttt acgtcgaatt tataacccag ttccttagcg gtggggacat ctggcaccca
     4861 cggccaacgg atgtcggaga aagaggccag aaggcgaaac cttccggcct tgatgtaaga
     4921 gaccacatct cctgggttct gggagacgat gtctacgtgt ccgcccaggc aggccgccac
     4981 cgcagccgga gtctccttga agaccaccgg ctcccacttg attccttcgg ctttggccag
     5041 ataaaccatc ccgaagttat tcggcgtggg taatccaatc aggctgtatt tgactttacc
     5101 ggggttcgcc ttggcgtatt gtaccaaatc cttgagagtc ttgaattggg aatcggagcg
     5161 ggcgacaggc ccgtacatgt aacggccgta ccccatgatg tattcaaaat ccttcaaagg
     5221 atcataaggg agatcgttca tgtgaggggc aaaagtcagc gggccgtaag tggttacccc
     5281 gatcgtgtag ccatccggct tcgaggcagc tatggccgcc atgcccacgg ttccccctgc
     5341 accggcccgg ttcaccacgg caacgggttg tcccagagcc ttctcggcct ttttcgccag
     5401 tgcacgggcg ctcaggtccg cccctcctcc cgccgagtag ttggcgatca aggttatggg
     5461 cctttccgga tattccgccc agacctggct tcccacgccg gtcagcgtta ccgatgccag
     5521 aaacgccaac aggaatcttc ttttcatttc tctcctccct cctgatgaaa gttgatgggt
     5581 gaatcggatt atttcttgat ggggaaccca aatcagccat tcaacccgtt tttctcacca
     5641 ctcaccacgc aaccttgagc attgcccatt gcccattgat tattgttatt actctgccat
     5701 tgccggcctc ttcttcccca gccaaggtag aaaaggggaa atgaccagca ggagggcgac
     5761 cccgattccg acggccgaga tcggccgagt gaagaaaata gaaaaatgcc cgtcggacat
     5821 ggttagggac tgacgcagag acatttccag caaggggcca aggacatatg cgagaaccag
     5881 aggggctccc tcatactcga atttcttcat caggtagcca aagaccccga aaacgatcat
     5941 gatgaggacg tccatgacgc tgtagttgat gctataggcg ccgatcaggc agaatagcag
     6001 gatcaaagga aagaggatcc ggtaggggac cttcagcacc tgcacccaca agccgatcaa
     6061 gggcagattc aggaggagga gcatgccgtt gcccacgtac atgctggtca ccactcccca
     6121 gaaaaggtcg ggatgactct ggaggagcat gggaccgggc tgcatgccgt gaatgaggag
     6181 ggccccgaag agcatggcca tgaccacatt cgcggggatt cccagagtca gaagggggat
     6241 gaaggcaccg ccagtggccg cgttgttggc cgtctccggc gcggccacac cctcgatggc
     6301 cccctgtccg aaccgctcgg gggtcctgga gacctttttc tccagggcgt aagagacaaa
     6361 ggaagagatc aaggctcctc ctccagggag gat
//
LOCUS       DASPRR010000346        10941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2335897, whole genome shotgun sequence.
ACCESSION   DASPRR010000346 DASPRR010000000
VERSION     DASPRR010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335897"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VEL68_13050"
     CDS             complement(<1..1024)
                     /locus_tag="VEL68_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ36938.1"
                     /translation="MNLNSLILHRTSRDQIQKGITVMAKAEGTHLVDQKGKRYLDLVS
                     GVTRPVHVGYGRKEIAQAVYDQVCEMSYITPMQFTNLPALRLAEVLKEIAPGKIDYFT
                     FVCDGSEAVETAIKLARHYHACRGDQKRYKVISRRGAYHGVTGGALRVLGTVMPMRQI
                     MEPLAPGTIFTESPYCYRCPQHLTYPACDLACAKDVERIIEFEDPTQVSAFIGEPIQQ
                     GFGAYAPPPDYWTIIRRTCDRYGILLIIDEVICGFGRTGEWFGVQHFAIQPDIMTMAK
                     GITSGYVPLGGVGCTEEVVKPIDVFQHLHTYGNHPVSCAAALKNIEIMKNENLILKSR
                     LMGGYFL"
     gene            complement(1098..1337)
                     /locus_tag="VEL68_13055"
     CDS             complement(1098..1337)
                     /locus_tag="VEL68_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HXZ36939.1"
                     /translation="MVAGVLIRTAPQRSKEVYWKLKNMEGVANIVRVFGQYDLVLMIR
                     ALDIDAAGKLVAKIREIEGVSYSETLIAAPPPDQV"
     gene            complement(1359..2366)
                     /locus_tag="VEL68_13060"
     CDS             complement(1359..2366)
                     /locus_tag="VEL68_13060"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ36940.1"
                     /translation="MLAVVKDFSQKGISLREVSQPEFSPYEVLIRVRAVGICGSDVRI
                     YNEVLPGRRKIIIGHELSGEIAELGEKVHSFRKGDRVATAICIGCAICRYCRKGYFNL
                     CDKLEEIGVTIDGGMAEYVSVPARNVHRVPEHVSFEEATLADPLSCSIRGLEMVSIQK
                     DSWVSILGPGTIGLLAVQIAKRVLRARVIVTGTQDDRLSLAQQFGADEVVNVNRQDPV
                     AFILEKTDGGSDFSFEATGSVQALENAFLSTKRNGSIVAMTVHKRVQINMEPVIRNEL
                     KVFGSICYNYKEFDQALDLIAKRKVDLELFTRHTYPLKEYEKAFAFAISRQGVKAILK
                     P"
     gene            complement(2453..3736)
                     /gene="hemL"
                     /locus_tag="VEL68_13065"
     CDS             complement(2453..3736)
                     /gene="hemL"
                     /locus_tag="VEL68_13065"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HXZ36941.1"
                     /translation="MKRKKSIALYQKALGMIPGGVNSPVRAFKAIGIPPTFIDRGKGS
                     KIWDIDGNEYIDYVGSWGPMILGHAHPRVVAALKKRAPQGTSFGAPTVLEVELAQRVK
                     KAFPSIDLVRMVSSGTEAVMGAIRAARGYTGRDKVLKFEGCYHGHGDSLLVKAGSGAM
                     TFGIPDSLGVPEDLAKHTLTAPFNNLQAVKNLVGQYPGQIACLIVEPIVGNMGVVLPE
                     KGFLEGLREICDQERIVLIFDEVITGFRIAYGGAQEIYQVKADLTCLGKIIGGGLPVG
                     AYGGKLAIMEKVAPLGGVYQAGTLSGNPMAMTAGIETLDILKAKKVYRDLAGKTGYLT
                     EAISQKAKERGIPVTINHAAGLFTLFFAEGPVKDYETAKKSDTQRFAQFFIHLMEQGI
                     YLPPSQFEAWFVSLAHSQKDLQRTIEACDAAFQKI"
     gene            4246..5067
                     /gene="ccsB"
                     /locus_tag="VEL68_13070"
     CDS             4246..5067
                     /gene="ccsB"
                     /locus_tag="VEL68_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HXZ36942.1"
                     /translation="MNIFFFHFSSFLYFLGTLAYLGYILFLKEVLSKLGMIAISLGFA
                     LHSLALLTRYGEAGHTPVTNLYESLSFFAWMIMGLLLVVQGRYKVKVLGAFLSPIALI
                     LMLFALALPEEIIPLAPVLKSFWHPFHVVFAFLGNTFFALAFCCGLMYLIQEHLIKTK
                     NVGALARRLPSLQVLDDLNYQSLAWGFPLLTLGIITGAIWAEYAWGRYWDWDPKETWS
                     LVTWFLYAALLHQRLAVGWRGRRAALMAIAGFLAVLFTFLGVNLLLPGLHSYSNW"
     gene            5076..6380
                     /gene="hemA"
                     /locus_tag="VEL68_13075"
     CDS             5076..6380
                     /gene="hemA"
                     /locus_tag="VEL68_13075"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXZ36943.1"
                     /translation="MELIVLGLNHKTAPIELRECLAFPEGELEKALSKAQSYPSLKEN
                     MILSTCNRVEVYAAVRETERALLDLKTFLAQYHQLPLKEFESALYSHTGEGAVRHIFR
                     VASSLDSMVVGEPQILGQLKDAYELAAGCKTSGLILHRLLHSAFHVAKRVRTETKIGN
                     SSVSVSSVAVDLAGKIFETLEEKTVLLIGAGEMAELAAQHLVSGGIKKVLVTNRTYER
                     AVSLAEKFQGEAVSFEAIDRGLKRADIVISATDSSHFILGYEQVARVMKERKQKPIFF
                     IDIADPRDIEPRVDDLENVYLYNIDHLQKVASDNIQDREKEAQKAEGIVQDELAKFVL
                     WYRSLSLIPTISALKGKFEEIRKKELEKALSHLPDLSDKEKNALEGLTSAIINKILHP
                     PFSLLKKADEEGNAARYLDALQALFQLSVSNADSSPQQRDEK"
     gene            6515..8122
                     /locus_tag="VEL68_13080"
     CDS             6515..8122
                     /locus_tag="VEL68_13080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ36944.1"
                     /translation="MKVKFMGAARTVTGSCYILETQGHRFAVDCGMHQGVGEVEKRNW
                     DTDIYEPHRIEFFLITHAHIDHSGLLPIMVKKGFRGKIYTTPPTRDLLEIMLLDSAHI
                     QETEAQWKSKKRLRHGESRTQPLYTQEDARAVIPLFVAKKYNEPFDLFPGLKVNFQDA
                     GHILGASVVELWVKESGEESKVVFSGDIGRPAQLLVEDPSQIRLADYLFLESTYGDRD
                     HKDETQSLNELAEAIAFSYARKEKVIIPSFAVERAQEVIYSLYLLSKDGRLPKDMPVY
                     LDSPLAIQTTEIFRRYKEYLDESTREILQKGEDPLSLPQLRYCQTPQESMSLNDLKGP
                     AIIISASGMAESGRIRHHLRHNLWREGASVVFVGFQALGTTGRKIVEGAKTVRLFNEE
                     IAVKAKVYTINGFSAHAGQSQILDWLGHFQTRDMKIILIHGEYKAQQILAELIRKRLG
                     CEVCIPDYLEEITLELGEKLRRVESPEKAVSRIDWAFLLQDLEAKLAQLRSRTAQLEA
                     KELAEQAELRDRLLELNRDLSEIVSET"
     gene            complement(8268..9092)
                     /locus_tag="VEL68_13085"
     CDS             complement(8268..9092)
                     /locus_tag="VEL68_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HXZ36945.1"
                     /translation="MSHDPIRFRLTINLIILLAVLVLGTLGFMYLENRSLLDAFYFII
                     VTLGSVGYGDIHPTTPSAKVFVIFLIILGVGTFVGVLASGTEMILSRREKLSRMNKLN
                     MVIGVFFSEVGNRLIRMFAQADPRFEEIQKGLIITENWTHQDFLMVRDHLKGMKYGVE
                     IAQTDLAALKDFLIKQRDFLVRLLENPTLLEHESFTDLLRAVFHLAEELAYREDLSQQ
                     PGPDRTHIAGDINRAYHFLVHQWVDYMEYLKVNYPYLFSLAMRTNPFDRQASPIVK"
     gene            complement(9089..9790)
                     /locus_tag="VEL68_13090"
     CDS             complement(9089..9790)
                     /locus_tag="VEL68_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36946.1"
                     /translation="MDIIRQLERLAQKLGDLGDEIVIVGGCSPALILDINLAPDLRPT
                     NDIDLLVKTESYGKYVLFVERMKEKGFTERQGDPIGRYVWEDLVVDVIPTETGVLGFS
                     NPWYKEAFDKAIKKRLPSGRVLRTITPIYFLATKLEAFRTRGEGDPLSSPDLEDIIAV
                     MVEFPAFEEELKEASPDLRQYISAQFRRLTQLADYPHFLSAHLRGDEASQAFLPRLRV
                     IFGRISSLTYGVTAS"
     gene            complement(9760..10272)
                     /locus_tag="VEL68_13095"
     CDS             complement(9760..10272)
                     /locus_tag="VEL68_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015214269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36947.1"
                     /translation="MLLKLVAIGDKKFQIYPLAHELAISPSELSASIRRSIYAGLIDP
                     MERRCNLPSFVEFMLHGFQYVFPVKPGEITRGMPTAHSATPLKEKILSNVDYVWPMPG
                     GTVRGQAIKPIYKSVPYAAQNDSALYRLLALADALRVGLARERKIASDIFKELILKEN
                     PWTSSDSLNG"
BASE COUNT         2500 a         2991 c         2931 g         2519 t
ORIGIN      
        1 cgaggaaata accccccatg agcctggact tcaagatcag gttttcattt ttcatgatct
       61 cgatgttttt cagggccgcg gcgcaggaga cgggatggtt cccgtaagtg tggagatgct
      121 ggaagacgtc gatgggcttg accacttcct ccgtacaccc gactcctccg agggggacgt
      181 acccgctggt aatccccttg gccatggtca tgatgtccgg ctggatggcg aaatgctgga
      241 ctccgaacca ttccccggtt ctcccgaatc cgcagatgac ttcgtcgatg atgaggagga
      301 tcccgtagcg gtcgcaggtc cggcggatga tcgtccagta gtcggggggc ggagcgtaag
      361 ccccgaagcc ctgctggatg ggttctccga taaaggccga aacctgggtg gggtcctcaa
      421 actcgatgat ccgctccaca tccttcgcgc aggcgaggtc gcaggccggg taggtgagat
      481 gctggggaca gcggtagcag taaggagatt cagtaaagat ggtccccggc gccaggggct
      541 ccatgatctg ccgcatgggc ataacggttc ccagcacgcg cagggctccg ccggtgaccc
      601 cgtgataggc tccccgccgg gagatgacct tgtaccgttt ctggtcgcct ctgcaggcat
      661 ggtaatgccg ggccagcttg atggccgttt ccaccgcctc tgagccgtcg caaacaaaag
      721 tgaaataatc gatcttcccc ggggcgatct ctttcaaaac ctcagccagc cggagggccg
      781 ggaggttggt aaactgcatg ggggtgatgt aggacatctc gcagacctgg tcgtagaccg
      841 cctgggcgat ctccttccgg ccgtatccca catggaccgg acgggtcact ccggagacca
      901 ggtcgagata gcgtttcccc ttttgatcca ccaggtgcgt tccttccgcc ttggccatta
      961 cggtgatccc cttctggatc tgatcccggg aagtgcggtg cagaataagg gagttcaagt
     1021 tcatcttggg tttctccttt taaaatgggc tgaaatgata tttttgctga cggggtcaaa
     1081 acccgccgcc tttgttttca cacttggtcc gggggaggag cagcgatgag ggtttccgaa
     1141 tagctgaccc cttcgatctc ccggattttg gcgaccagct tgccggcggc atcgatgtcc
     1201 agggcgcgga tcatcagcac caggtcgtac tgtccgaaaa cccggacgat gttggccacc
     1261 ccttccatgt tttttaattt ccagtacacc tctttgctcc tctggggagc tgtccggatc
     1321 aatacgccgg caaccatttt gactcctcct gtcgcaggct atggtttgag aatcgcctta
     1381 accccctgtc tcgatattgc gaaggcaaag gccttttcgt attctttcag gggataggta
     1441 tgtcgggtaa acaactcgag gtccaccttc ctcttggcga tcaaatccag ggcttggtcg
     1501 aattccttgt agttgtagca gatggaaccg aatactttca gctcattgcg aatcaccggt
     1561 tccatattga tctggaccct tttgtgaacc gtcatggcca cgatggatcc gttccgcttg
     1621 gtggaaagga aagcattctc cagggcctgt accgatccgg tggcttcgaa agaaaaatcg
     1681 gacccaccgt cggtcttctc caggatgaaa gcgaccgggt cctgacgatt cacgttgacc
     1741 acttcatcgg ccccgaactg ttgggccaag gacaggcggt catcctgcgt cccggtgacg
     1801 ataaccctgg ccctcaggac ccgcttggcg atctgcacgg ccagcaggcc gatggtcccc
     1861 ggacccagaa tggagaccca ggaatccttt tgaatactga ccatctccag cccccggatg
     1921 gaacaggaca aagggtcagc cagagtggcc tcttcgaagg agacatgctc ggggacccgg
     1981 tgaacattgc gggcgggaac ggaaacatac tccgccatgc ccccgtcgat ggtgactcct
     2041 atttcctcca gcttatcgca gagattgaaa tatcccttgc ggcaataccg gcagatggcg
     2101 cagccgatgc agatggcggt ggcgacccgg tccccctttc ggaaactgtg gaccttttcc
     2161 cccaattcgg caatttcccc ggaaagctcg tggccgatga tgatcttcct ccgcccggga
     2221 aggacctcgt tgtagatgcg aacatcgctc ccgcagattc ctacggctct cacccggatc
     2281 aggacttcat agggactgaa ttctggctgg gagacctccc gcagagagat gcctttctgg
     2341 gaaaagtcct tgactacggc cagcatcttt accttccttg gccaaccaat ctttcgggtt
     2401 gcacagtccc catgccgggg cctaacccct tccgggcaag cgagagcgga cctcaaattt
     2461 tctgaaaggc ggcatcgcag gcttcaatgg ttctctgcaa atctttctga gagtgagcca
     2521 gcgatacaaa ccaggcctca aattgggagg gagggagata aattccttgc tccatcaggt
     2581 gaatgaaaaa ctgggcgaat cgttgggtat cgctcttttt ggcggtttca tagtctttca
     2641 ccgggccctc ggcaaagaag agggtgaata gtccggcggc atggttgatc gtgaccggga
     2701 ttcctctttc tttggccttc tgggaaatgg cttcggtgag atatccggtc ttcccagcca
     2761 aatcacggta aacctttttc gccttgagaa tatccagggt ctcgatcccg gcggtcatgg
     2821 ccatgggatt gccggagagg gtcccggcct gataaacccc cccgaggggg gcgacctttt
     2881 ccataatcgc cagtttgccg ccgtaagcgc ccacgggcag gcctccgccg atgatctttc
     2941 ccaggcaagt cagatcggcc ttgacctgat agatttcctg agcgcctcca taggcgatcc
     3001 tgaatccggt aatcacctcg tcgaagatga gcactatcct ttcctgatca cagatttccc
     3061 tcaatccctc caggaatccc ttttccggaa ggaccacccc catgtttccc acgatgggct
     3121 ccacgatgag gcaggcgatt tgcccgggat attggccaac caagttttta accgcttgga
     3181 gattattaaa gggagctgtc agagtatgtt tggcaaggtc ttcggggacc cccagactgt
     3241 cgggaatccc gaaggtcatg gctccggaac cagccttgac caggagactg tcgccatggc
     3301 catggtaaca gccttcaaat tttaggacct tgtcccttcc cgtatagcct ctggccgcgc
     3361 gaatggcccc catgaccgcc tcggtaccag agctcaccat tctcaccaaa tctatcgagg
     3421 gaaaagcctt cttcactctc tgggcgagtt ctacttcaag aaccgtggga gccccaaagc
     3481 tggttccctg gggggccctc ttctttagcg ctgccaccac ccgaggatgg gcatggccca
     3541 ggatcatggg gccccaggac cctacgtaat caatatactc gtttccatct atgtcccaga
     3601 ttttcgagcc ttttcctcgg tcgatgaaag tggggggaat cccgatggcc ttaaaggctc
     3661 ggaccgggct gttcaccccc ccggggatca ttcccaaggc cttttggtag agcgcaattg
     3721 attttttcct tttcatcgcc cttcctcctt ccaggttggg tggctaaatc atacctatca
     3781 atcatgggta gtcgattggc aaccagaaat caccccaaga gggtgaaatc agtatagaaa
     3841 aagggggtgg gtgggtcaag gggaatttga attttgccta ccttcccttt taaggtaact
     3901 tttctcgtgg ctcgggtaca aggtccgttt ctggcaggac aggatcggcg ggggttagag
     3961 gaccgatgga agatcgcttc ccttccgctg agagaggcgg gaaaggcata accatctgct
     4021 cccacaggtc catttccgcc cttgccgatg aaagaaaatg cgccttatca tgtcctggca
     4081 gaaacggacc ttgcacccgg gccaggcaag gcctgcgagt ttatggctta caactggttt
     4141 tcggaaagtg agaagaggaa agaattttga atgccagact tgagttgacg aggccacttt
     4201 cctcgattaa cataataata cgatctttgt attctctgtc ctcccatgaa tatttttttc
     4261 ttccattttt cttcctttct ttatttcctg ggaaccctgg cctacctggg gtatatcctc
     4321 tttcttaaag aggtcttgtc caagctggga atgatcgcta tctctttagg ttttgctctg
     4381 cacagcctgg ccctcctgac ccggtacggc gaggccggcc ataccccggt gaccaatcta
     4441 tatgaatccc tctctttttt cgcctggatg atcatgggac tacttctggt cgtacaagga
     4501 aggtacaagg tgaaagtcct gggggctttc ctgtccccta ttgccctgat cctcatgctt
     4561 tttgccctgg ccctgcctga ggagattatc cccctggctc cggtcctcaa gagcttttgg
     4621 cacccctttc atgtggtctt tgcctttttg ggaaatacct tctttgccct ggccttctgc
     4681 tgcggactca tgtatctcat ccaggaacac ctgatcaaaa caaaaaatgt aggggccctt
     4741 gcccggagac ttccatcgct ccaggtgctg gatgatctga actatcaatc tctcgcttgg
     4801 ggatttcctc tcttgaccct gggaatcatc actggggcca tttgggcaga atatgcctgg
     4861 gggcgctact gggattggga cccgaaggaa acctggtccc tggttacctg gttcttatat
     4921 gccgccttgc tccatcagcg tctggccgtg ggttggaggg gtcgcagggc agccctcatg
     4981 gccattgcgg ggtttctggc cgtcctgttt accttcctgg gggtcaacct tctgctcccc
     5041 gggcttcatt cctattccaa ctggtagcgg gcaagatgga actcatcgtc ctcggattaa
     5101 accataaaac tgcccccatc gaattgcggg aatgtttggc ctttcccgaa ggggagttgg
     5161 aaaaagccct cagcaaggcc cagagctatc cgtccctgaa agaaaacatg attctctcca
     5221 cctgtaatcg ggtggaagtc tatgccgccg tgcgggaaac cgaaagagcc ctcctcgacc
     5281 tgaaaacctt ccttgcccaa taccaccaac tgccgttgaa agaatttgaa tccgccctct
     5341 attcccacac cggggaaggg gcggtccggc atatcttccg cgtagcttca agcctggatt
     5401 ccatggtggt gggggagcct caaattctgg gccagctcaa ggacgcctac gagttggccg
     5461 caggatgtaa aacctccggt ctgatccttc accggctcct gcacagcgcc tttcatgtgg
     5521 ccaaaagggt ccggacggaa accaagatcg gcaacagctc tgtgtcggtc agctccgtgg
     5581 ccgtcgatct ggccgggaag attttcgaaa ccctcgagga aaagacggtc cttttgatcg
     5641 gcgcgggcga gatggctgaa ttggccgccc agcatctcgt atcggggggg ataaaaaaag
     5701 tcttggtcac caatcggacc tacgaacggg ctgtgagcct tgccgagaaa ttccaagggg
     5761 aagccgtttc cttcgaggcg atcgaccggg gattgaaacg ggccgatatc gtcatcagcg
     5821 ccacggattc gagccacttc atccttggct atgaacaggt ggccagagtc atgaaggagc
     5881 ggaagcaaaa gcctattttc tttatcgaca tcgccgaccc ccgggatatc gagcccaggg
     5941 ttgatgacct ggagaacgtc tacctgtaca atatcgatca cctccaaaag gtggccagtg
     6001 acaacatcca agaccgggaa aaggaagccc aaaaagcaga gggcatcgtt caagacgaat
     6061 tggccaagtt tgtactctgg taccgatccc tgtccttgat ccccaccata tccgccctga
     6121 agggtaaatt cgaggaaatc cggaagaagg aactggaaaa ggccctttcc catcttccag
     6181 atttgtcgga taaggaaaag aatgccctgg aaggtctcac ctccgccatt atcaacaaaa
     6241 tcctccatcc ccccttcagc cttctgaaaa aggccgacga agaagggaat gcggcccgtt
     6301 acctggatgc cctccaggcc cttttccagc tttcggtttc caacgccgat tcatctccgc
     6361 agcaaaggga cgaaaaatag ccaaaaagag gcaaaacaaa ttttctgctc cgcccccggg
     6421 gaagccattc ccaaccggga gataaggttg gggtggaaga ctccgattcg gtcctaaggg
     6481 gaggaagaaa gacaaactgt ggaagaggga agacatgaaa gttaaattta tgggggcggc
     6541 gaggacggtc acggggtcct gttatatcct ggaaacccag ggacaccgat ttgccgtcga
     6601 ctgcggcatg caccagggag tgggagaggt agagaagcga aactgggaca cggatatcta
     6661 cgaaccccac cggattgagt tttttctcat cacccatgcc catatcgatc actccggtct
     6721 cctgccaatc atggtcaaga aggggttccg ggggaagatt tacaccaccc cgcccacccg
     6781 ggacctgttg gagatcatgc tcctggacag cgcccacatc caggagacgg aggctcagtg
     6841 gaagagcaaa aagcgactcc gccacgggga gagccggaca cagccccttt acacccagga
     6901 ggacgcccgg gccgtcattc ctctttttgt ggccaagaaa tacaacgaac ccttcgacct
     6961 cttccccggg ctaaaggtga atttccagga tgccggccac atcctggggg cctcggtcgt
     7021 ggagctctgg gtgaaagaaa gcggggagga gagcaaggtc gtattctccg gggacattgg
     7081 acgccccgct cagctcctgg tggaagaccc gagccaaatc cggctggcgg attacctttt
     7141 tctggaatcg acctacggcg accgggacca taaagatgaa acccaaagtc tgaacgaact
     7201 cgccgaagcc atcgccttca gctatgcccg caaggagaaa gtgatcattc cgtcctttgc
     7261 cgtggaacgg gcccaggaag tcatctattc tctctacctc ctttccaagg atggaagact
     7321 cccgaaagac atgccggtct atttggacag ccccctggca attcaaacga ccgaaatctt
     7381 tcgccgctat aaggaatacc tggatgagtc cacccgggaa attctccaaa aaggcgagga
     7441 ccccctttct ttgccccagc tccgctactg ccagacccct caagagtcca tgtccctcaa
     7501 tgacctgaag gggccggcga tcatcatctc ggcgagcggc atggccgaat ccggccggat
     7561 ccgccatcac ctgcgccata acctctggcg ggaaggggcc agcgtggttt tcgtcgggtt
     7621 tcaggccctt gggacgaccg gccgcaaaat cgtggagggc gccaagacgg tccgtctctt
     7681 caacgaagag attgcggtga aggcgaaggt ctacactatc aatggttttt ccgcccatgc
     7741 cgggcaaagc cagattctgg attggctggg gcactttcaa acccgggaca tgaaaatcat
     7801 cttgatccat ggagaataca aggcccagca aatcctggct gaattaatcc ggaagcggtt
     7861 gggatgcgag gtttgcattc ccgattatct ggaagaaatt accctggagc tgggagaaaa
     7921 gctccggaga gttgaatccc cggagaaggc cgtctcccga atcgattggg ctttcctctt
     7981 gcaggacctg gaggcaaaac tagcccaact tcgcagtcgg acggcgcaac tggaggcgaa
     8041 ggaattggcg gaacaggcag aactccggga ccggcttctg gaattgaacc gggacctgtc
     8101 ggaaatcgtg tcagaaacct gagaaatgtg ggccggtttg aatcagagaa gcccggttca
     8161 gccatgattg cggaactctg aaaaaagtgg aggaagaaat tccgggggtc gcgctgaata
     8221 tcagctcggt aatggaattt cgaaggtcga tcaaataatg gggagaatta tttcacgatg
     8281 ggggaagcct gacggtcgaa gggattggtc cgcatggcca gggagaaaag atagggatag
     8341 ttgaccttca agtattccat gtaatccacc cactggtgaa cgaggaaatg gtaagcccgg
     8401 tttatatccc cggcgatgtg ggtccggtcg ggtccgggct gctggctaag gtcttcccga
     8461 taggccagct cctcggcgag atgaaagacg gcccgcagca gatcggtgaa ggattcgtgc
     8521 tccagcaggg tggggttttc caggagcctc accaaaaaat ccctctgttt gatgaggaaa
     8581 tccttcagtg ccgccaagtc cgtttgagca atctccaccc cgtatttcat cccttttaaa
     8641 tggtctctga ccattagaaa atcctggtgg gtccaattct cggttatgat cagtcctttc
     8701 tggatttctt caaaacgggg gtcggcctgg gcgaacatac ggatcagccg gtttcccact
     8761 tcgctgaaga aaaccccgat taccatgttc agcttattca tgcggctcag tttttcccgc
     8821 cggctgagga tcatttccgt gccactggcg agcactccca caaaagtgcc tacccccaaa
     8881 atgatcaaaa aaataacgaa tacctttgcc gatggagtgg tggggtggat atctccataa
     8941 cccacggatc ccagggtaac gatgatgaaa taaaaagcat ccaagaggga tcggttttcg
     9001 aggtacatga agcccagggt ccccagcacc aaaactgcca agaggattat caggttgatg
     9061 gtcaaacgaa atcgaatggg atcgtggctc atgatgcggt aactccataa gtcaatgagg
     9121 agattctccc gaagattacc ctgagtctgg gcaaaaaagc ctggcttgct tcatcccccc
     9181 tcaaatgggc ggaaagaaaa tgggggtaat ccgctaactg ggtcaacctc ctaaattggg
     9241 cagaaatgta ctgccgtagg tccggggaag cctctttcag ctcttcttca aaggcaggaa
     9301 attcaaccat caccgcaatg atatcttcca agtctgggct tgataaggga tcaccttcgc
     9361 cccgagttct aaaagcctcc agcttggtag cgaggaaata aatcggggtt atcgtcctca
     9421 gaacccttcc gctcggtaat cgcttcttga tggctttatc aaaggcctct ttgtaccaag
     9481 ggttagaaaa tccaagaacc ccggtttcgg tgggaatcac gtccactacg aggtcttccc
     9541 acacataccg gcctatagga tcgccttgcc tttcggtgaa gcctttctcc ttcattcttt
     9601 caacaaaaag gacatatttc ccatagctct ctgttttgac tagcaagtct atatcattag
     9661 taggccgcag gtccggggcc aaatttatgt ctaagatcag ggccggtgaa caccccccta
     9721 cgatgacaat ttcatcgcca aggtcgccga gcttctgggc taaccgttca agctgtcgga
     9781 tgatgtccaa gggttttcct tcaaaatcaa ttctttaaaa atatccgagg ctattttcct
     9841 ttctctggcc agaccgaccc gcaaagcatc agccagggcg agtaatcggt aaagcgccga
     9901 gtcattttga gcagcataag ggaccgattt atagatgggt tttatggcct gtcccctaac
     9961 tgtcccccct ggcatgggcc aaacataatc aacattcgac aatatctttt ctttgagcgg
    10021 agtagccgaa tgggcggtgg gcattccgcg ggttatttct cccggcttga ccgggaagac
    10081 atattgaaac ccatgcagca tgaattcaac gaatgacggc aggttgcatc tcctttccat
    10141 gggatcgatt aggccggcat agatggagcg ccggatagag gcgcttagtt cggaagggct
    10201 aatagccaat tcgtgggcga ggggataaat ctggaacttc ttatccccta tggcaaccag
    10261 ttttaaaagc acaactatat cttgcccttt catgatctat attttattcg caattcgcga
    10321 attgtgcaac atatttttat tattaaataa attttattat caaggcccgc ataaaggtca
    10381 tgcaggaatt agcgaattct catccgaaac ccaaattatt atcccctccc cctcgatggg
    10441 ggggagggaa gggtgggggt gacaatggcc ttgaattgca gaattttcct tttaaattcg
    10501 ccctcacccc gaccgttcgg ctgagctcac gactaaaccc tctcccgcta gaggctgtat
    10561 cgtaatccgt tcatggttcg acaagctcac cacgaacgga ttatgagcgc attaaaattt
    10621 agccacttgg ccgctcgcct gctcggctga gctcgcgacg aagcctgagc ctgtcgaagg
    10681 gcgaccgggg aattacgaca cagtttgcta ggagagtggg gtgtcctgat aggtactggc
    10741 tagcagattg taggggggcg gccccatgca gcggcctgaa aaatgtgctg tattgccaga
    10801 gaccggcttt ttcccgtgac ggagaaaaat tccgtcaaac agggtagccg cgctttccct
    10861 gcaatccttt gaggcaggaa atctctccgc tgtgggtgag cgaattcccc aaaatcccgt
    10921 tgatcagcac gtacccaagg g
//
LOCUS       DASPRR010000347         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1695569, whole genome shotgun sequence.
ACCESSION   DASPRR010000347 DASPRR010000000
VERSION     DASPRR010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695569"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VEL68_13100"
     CDS             <1..148
                     /locus_tag="VEL68_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36948.1"
                     /translation="IVLVEHNMHLVMGISDRLVVLHHGSKIGEGRPEEIQKDARVIEA
                     YLGK"
     gene            236..967
                     /locus_tag="VEL68_13105"
     CDS             236..967
                     /locus_tag="VEL68_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36949.1"
                     /translation="MLKLKNIEAFFGKVQVLRHVTLHVDAGEIVSLIGANGAGKTTLL
                     NVISGVHPSWEGSRVFNEVETRGWKAEKLVARGIVQIPEAEKVFTPLSVLENLELGAY
                     LRRLGRAALQEEMEKAFAVFPILRERKNQPAGTLSGGERQMLALGKALMARPKLIMPD
                     EPSLGLAPTVVAEIFHIIRSLNEQGATILLVEQNARETLRISHRAYVLDTGRIVLSGT
                     GEDLLNNEEVKRAFLGKDYQGKWER"
     gene            980..>2143
                     /locus_tag="VEL68_13110"
     CDS             980..>2143
                     /locus_tag="VEL68_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HXZ36950.1"
                     /translation="MPIFNPAVETMDRKALLDLQNERLQIVVNQAYSNVEFYRRKFDE
                     AGLSPDDIKTLEDLERIPFTTRKDLMENYPYGMFAVPLRDVVRLQFPLGLYGNPIVIG
                     YTRKDLEVWRELVARIMAGIGITEHDIIQVAFNYGLFLGAIRFNQSAELIGAAVVPAS
                     IAPAEVQLRIMKDFRSTVLASTPSFALHILEVMQKKGINPRTLSLKIGLFGAEPWPEE
                     VHRFIEANWGIKAYDIYGVMELVEPGVAGECMAQAGLHIFEDHFLPEIIEPDSGKPLE
                     PGKEGELVLTTLTTNAYPLLRYRTSDVTALHLEPCSCGRTLARMERVLTRSDQMITVR
                     GINVFPEKIQEVLASFQGIGPAYTTSVSKKKGMNDQMRIEMEASAEIMSAPEDR"
BASE COUNT          490 a          580 c          653 g          420 t
ORIGIN      
        1 catcgttctg gtggaacaca acatgcattt ggtgatgggg atctcggatc gcctggtcgt
       61 ccttcaccac ggctccaaga tcggcgaagg gcggccagag gaaatccaga aggatgcgcg
      121 ggtgatcgag gcatatctgg gaaaataagc tatcagcggt cagcgatcag ctttcagcta
      181 agaaatttgg ctgttggctg aacgctgatg gctgaatgct gatcgctgtc tttctatgtt
      241 gaaattaaaa aatattgaag ccttcttcgg aaaggtccag gttttgcgcc atgtgaccct
      301 gcacgtggat gccggggaga tcgtatctct gatcggggcc aacggggcgg ggaagaccac
      361 cctgctgaac gtgatctccg gggtgcatcc ctcctgggag ggaagccggg tcttcaacga
      421 agtggagacg cggggatgga aagcggaaaa gctcgtagcc cgggggatcg tccaaatccc
      481 ggaggcggaa aaagtcttca ccccgctgtc ggtcctggag aacctggagc tgggcgctta
      541 cctcaggcgc ctcgggcggg cggcgctgca ggaagagatg gaaaaggcct ttgccgtctt
      601 ccccattctc cgggagcgga agaatcaacc ggccgggacc ttatcgggcg gcgaacggca
      661 gatgttggcc ctgggtaagg cactcatggc ccgacccaaa ttgatcatgc ccgacgagcc
      721 ctccttggga ctggcaccga ccgtggtggc ggaaatcttt catatcatcc gctccctcaa
      781 cgaacagggg gccaccatcc tgctggtgga gcagaatgcc agggaaactc tgagaatctc
      841 ccatcgggcc tatgtcctgg acacggggag aattgtattg agcggcaccg gggaggacct
      901 cctgaacaac gaagaagtca agagggcctt cctgggaaag gattatcagg gaaagtggga
      961 aaggtagagg gaggagaaga tgcccatatt caacccggct gttgagacca tggatcgcaa
     1021 ggcgcttctg gacctgcaga atgagaggct ccagatcgtg gtcaaccagg cctactcgaa
     1081 cgtagagttt taccgcagga aattcgacga ggccgggttg agtccggatg acatcaaaac
     1141 tctggaagac ctggaaagaa ttcccttcac cactcggaaa gacctgatgg agaactaccc
     1201 ctacggcatg tttgccgttc ccctgcggga cgtggtccgc ctgcagtttc ccctgggtct
     1261 ctacgggaat cccattgtca tcggctacac ccgtaaagac ctggaggtct ggcgggagct
     1321 ggtggcccgg atcatggccg gaatcgggat caccgagcac gacatcattc aggtagcttt
     1381 caactacggc ctcttcctgg gcgcgatccg gttcaaccag agcgcggagc tgatcggagc
     1441 ggcggtcgtt cccgcctcca ttgccccggc cgaggtccag ctgcggatca tgaaagattt
     1501 ccggagcacg gttctggcgt ccaccccttc ctttgcgctg cacattctgg aggtcatgca
     1561 gaagaaaggg attaatccgc ggaccctctc cctgaaaatc ggccttttcg gagcggagcc
     1621 ctggcccgag gaagtccacc gattcataga agccaactgg gggatcaaag cctacgacat
     1681 ttacggggtc atggaattgg tggaaccggg cgtggccggg gaatgcatgg cccaagcggg
     1741 attacatatt tttgaggatc attttctccc tgaaatcatt gagccggatt cgggcaaacc
     1801 cctggagccg ggaaaggaag gagaattggt gctcaccacc ctgaccacca acgcctaccc
     1861 cctcctgcgc taccggacga gcgatgtcac cgccctacat ctggaaccct gctcctgcgg
     1921 ccgcaccctg gctcggatgg aacgggtgtt aaccaggtcg gaccagatga tcaccgtccg
     1981 ggggatcaat gttttccccg agaaaatcca agaggtcctt gcttctttcc aggggatcgg
     2041 cccggcctac acgacatcgg tgtccaaaaa gaagggaatg aacgatcaaa tgaggattga
     2101 aatggaagct tctgcggaga ttatgagtgc ccccgaagac cga
//
LOCUS       DASPRR010000348         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_733922, whole genome shotgun sequence.
ACCESSION   DASPRR010000348 DASPRR010000000
VERSION     DASPRR010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733922"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..495
                     /locus_tag="VEL68_13115"
     CDS             127..495
                     /locus_tag="VEL68_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXZ36951.1"
                     /translation="MPTKAAKIFQDLIVWQKAHLLVLSVYRLGENFPRREIYGLTLQL
                     RRAAISIAANIAEGFKKKTKLEKLRFMNIAQGSTEECRYYLILAKDLGYGNGEECMAL
                     LEETSNLLEAYSNSIFRSGS"
     gene            521..1273
                     /locus_tag="VEL68_13120"
     CDS             521..1273
                     /locus_tag="VEL68_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36952.1"
                     /translation="MPELIRVEHLTKIYYLGEVQVPALKGMDLTIEAGECVAIMGASG
                     SGKSTFMNILGCLDRPTQGKYFLEGKDVSGLSRDELAQIRNQKIGFVFQNFNLLGRTS
                     ALENVELPLYYRRDHSSRDRHRTATEALATVGLQAREHHLPSQLSGGQQQRVAIARAL
                     VNQPSLILADEPTGNLDTRTSLEIMAILQKLNQESRITIILVTHEPDVARFAGRHVVF
                     RDGKVIQDERIHARQASDDLARLPIPEEEEGR"
     gene            1270..2496
                     /locus_tag="VEL68_13125"
     CDS             1270..2496
                     /locus_tag="VEL68_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ36953.1"
                     /translation="MNFLMTLRIAFKALGRNKMRSGLTMLGIIIGVGAVIAMIAIGSG
                     AKARIQEQIASMGSNLLIVLSGSATSGGFRWGSGSVPTLTVDDSKAIANELSAVKYAA
                     PVLQGVAQIVFGNQNWATVTFATTPEALLIRDWAVVAGRPFSQSDIDGAAKVCLLGQT
                     VVENLFGEMDPVGQVVRIKKFPFTVVGVLADKGQTTWGQDQDDVVYVPLTTGQRLLFG
                     QQFPGMVRSISVQATGPEVMKAAEDQITQLLRQRHRLRANQDNDFFVRNLTEAFSTAE
                     ESARVMSILLGAIASISLLVGGIGIMNIMLVSVTERTREIGIRMAVGARGRDILWQFL
                     VEALVLSLIGGVMGIILGVGASQLISQMFKWPTLISLQSLLISFSFAGGVGIFFGFYP
                     ARKAAQMDPIEALRYE"
     gene            2725..3741
                     /locus_tag="VEL68_13130"
     CDS             2725..3741
                     /locus_tag="VEL68_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ36954.1"
                     /translation="MTSRERVLTALKIKQPDRVPWVESSIHNTVVEKLLKRTDFEKAN
                     VTQLFAMPGARFSTAVFEVLAIDNTNFSIAPPRFVKSQSFEGMDIISDGLIKTEADLA
                     KIVLPDPESDDFYKPAQEFVAKFKGSNKALGVTTRIGVSNTYLSMGIEHFSLMLYDNP
                     DFVLKVMDIFVDWACRAISKINNLGFDFMIVPEDLAWKQGPMFSPKVFRELFLPRMKK
                     AAEKIRIPWIYHSDGNLMPILDDLLTLGMNGLANIEPNAMDINELKKKYGRRVCLVGN
                     IDLHYTLTQGTPEEVEAEVKKRIQEVGSGGGYILASSNSIAGYCKPENVLAMHRALLK
                     YGNY"
     gene            complement(3778..>4026)
                     /locus_tag="VEL68_13135"
     CDS             complement(3778..>4026)
                     /locus_tag="VEL68_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ36955.1"
                     /translation="FRLTPEEALRAVTIDAAWQTFDENIKGSIEVGKLADFTILAENP
                     LAVPPEGIKDIKVEEVIIGGKPEIGRVELSGGNITGKK"
BASE COUNT          917 a         1099 c         1103 g          907 t
ORIGIN      
        1 atccttgaag ccatggccgg gaacagcaag agcggaagtc cctcgcaggc ctctcagccc
       61 tccatgaaag gcttccggta atagaaccag aactcaggag ccaggagtca gaatccagga
      121 aatattatgc cgaccaaagc ggcaaaaatt tttcaggatt taatcgtatg gcagaaggcc
      181 catcttcttg ttctttccgt atatcggcta ggtgaaaact tcccgagaag ggaaatatac
      241 gggttgacct tacagctccg acgagctgct atatctattg ctgcgaatat cgccgaaggc
      301 tttaagaaaa agacaaagct ggaaaagttg cgattcatga atatcgctca aggctccacc
      361 gaagaatgtc gttattatct gattttggcc aaagatctcg gctatggaaa tggtgaagaa
      421 tgcatggctc tgctggaaga aaccagtaac ctgcttgaag cttattctaa ttccatcttc
      481 cgttctggct cctgactcct gaattctgga ttctctcctt atgcctgagc tgatccgcgt
      541 cgagcatctg accaaaatct actacctggg ggaagtacag gtgccagccc tcaaggggat
      601 ggacctgacc attgaggccg gtgagtgcgt ggccatcatg ggggcgtcgg gatcgggaaa
      661 gtctaccttt atgaacattc ttggctgcct ggaccgtccc actcaaggaa aatactttct
      721 ggaggggaag gatgtctccg ggctgagccg ggacgagctg gcccaaatcc gcaaccagaa
      781 gatcggcttt gtcttccaaa atttcaacct cctgggccgg acttccgcct tggagaatgt
      841 cgaactccct ctttactatc gccgggacca ttcctcccgg gacagacacc ggacagccac
      901 ggaagccctg gcgacggtag gccttcaagc tcgggagcac caccttccca gccagctctc
      961 cgggggccag caacaacgag tggccatcgc tcgcgctctc gtgaaccagc cttctctcat
     1021 cctcgcggat gaacccacgg gtaacctaga tacccgaacg agtctggaga tcatggccat
     1081 tttgcagaag ctcaaccagg agtcccgcat caccatcatt ctggtgacgc acgaaccgga
     1141 tgttgcccgt ttcgcgggcc gccatgtagt cttccgagat gggaaagtga ttcaagacga
     1201 gcggattcat gcccggcagg cctccgacga tctggcccgg ctaccgattc ccgaggaaga
     1261 agaaggccga tgaattttct tatgaccttg cgcattgcct ttaaagccct gggacgcaac
     1321 aagatgcgct cgggcctcac catgctggga atcataatcg gcgtgggggc ggtcattgcc
     1381 atgatcgcca tcgggtccgg agcaaaggcc cgcatccagg aacagatcgc ttccatgggc
     1441 tctaatctcc tcattgtcct ttccggaagc gcgaccagcg ggggtttccg ctgggggtca
     1501 ggatcggttc cgaccctcac cgtggatgat tccaaggcga tcgccaacga gttatcggcg
     1561 gtaaagtacg cggccccggt ccttcagggg gtggcgcaaa tcgtttttgg gaaccagaac
     1621 tgggccacgg tcactttcgc caccacgccc gaggctctcc tcatccggga ttgggcggtc
     1681 gtagccggga ggcccttttc ccagtcggac atcgatggag cggccaaggt ttgcctgctg
     1741 gggcaaacgg tggtggagaa tctcttcggg gaaatggacc ccgtggggca agtggtgcgg
     1801 attaagaagt ttcccttcac cgtggtgggg gttctggcgg ataagggcca gacgacctgg
     1861 ggccaggacc aggatgatgt cgtttatgtt cccttgacca cgggccagag gcttctcttc
     1921 gggcaacaat ttccggggat ggtccgctcc atcagcgtgc aggccaccgg gccggaggtg
     1981 atgaaggccg ccgaggatca gatcacccag cttctgcggc agcgacaccg gctccgggcc
     2041 aaccaggaca acgacttctt cgtccgcaac ctcaccgagg ctttctccac ggcagaggag
     2101 tcggcccggg tcatgtccat tctcctgggc gccattgcct ccatttccct tctggtgggc
     2161 gggatcggaa tcatgaacat catgctggtt tcggtaaccg agcggacgcg ggaaattggc
     2221 atccgtatgg ctgtaggggc ccgaggtcgg gatattctct ggcagtttct ggtggaagct
     2281 cttgttctga gcctaattgg aggggtgatg ggaatcattt tgggagtggg agcttcccaa
     2341 ctcatctctc agatgtttaa gtggccgacc ctgatttccc ttcaatcctt attgatttct
     2401 ttctctttcg ccgggggcgt agggatcttc ttcggcttct accctgctcg caaagccgcc
     2461 caaatggacc ccatcgaagc cctccggtac gaataatagg gaacaattta tcggagaagc
     2521 tcgggcgccg tcgttaaatc tccgattctc ctccaggtat caaatctctc ctgtcccatt
     2581 tttccctttg cagatggcag agacccgctg taagcagaag gtttccttta ccatgcaagg
     2641 ggaggtcttt ttctccctgg aaatccatgc gaaaacgggt aaaatgggat acatatgggt
     2701 atccaaacct gctaggagga aagcatgacc tcgagggaaa gagtccttac ggcccttaag
     2761 attaaacaac ctgaccgcgt tccctgggtg gaaagcagca ttcataacac cgtggtggag
     2821 aaacttctaa aacgaacgga tttcgaaaaa gccaatgtaa cccagctttt tgccatgccg
     2881 ggcgcaaggt tctccacggc ggtctttgag gttctggcca tcgacaacac aaatttcagc
     2941 attgctccgc cacgcttcgt gaagagccaa agttttgaag ggatggacat catctccgac
     3001 gggctgatca agaccgaagc ggacctggct aaaatcgtgc tgcccgaccc ggagtccgac
     3061 gatttctaca aacccgcgca agaatttgtg gccaaattca agggaagcaa caaggccttg
     3121 ggagtaacca cccggatcgg ggtcagcaat acctacctaa gcatgggcat tgaacacttc
     3181 agcttgatgc tttacgacaa ccccgatttt gttctgaagg tcatggatat tttcgtagat
     3241 tgggcctgca gggccatttc caagatcaat aatctcggct tcgacttcat gattgttcct
     3301 gaggacttgg cctggaagca gggtcccatg ttttccccca aggtcttccg agagctcttc
     3361 ctcccccgca tgaagaaggc ggcggagaag ataagaatcc cctggattta ccacagcgac
     3421 gggaacctaa tgcccattct cgatgacctg ttgactctgg gaatgaacgg tctggccaac
     3481 atcgagccca atgccatgga catcaacgaa ttgaagaaga aatacgggcg ccgggtctgc
     3541 ctggtgggga atatcgatct gcactacacc ctgacccagg ggaccccgga ggaggttgag
     3601 gcggaagtga agaagcgtat ccaagaggta ggctctgggg gtggttacat cttagccagc
     3661 tccaacagca ttgctggcta ctgcaaaccg gaaaatgtgc tagccatgca ccgggcgctc
     3721 ctgaaatacg ggaactattg aaagccatgg gtaggggcaa ttcaaggatt gcccccacta
     3781 ttttttaccg gttatgtttc ctcccgataa ttcaacccgc ccgatttctg gttttcctcc
     3841 gataatgacc tcctcgacct tgatatcctt tatcccttcc ggcggcacgg caaggggatt
     3901 ttcggccagg atggtgaaat ccgccagctt tcccacctca atcgagccct ttatattttc
     3961 atcgaaggtc tgccaggcgg catcaatggt gacggctcga agggcctctt cgggggtcag
     4021 gcgaaa
//
LOCUS       DASPRR010000349         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1208472, whole genome shotgun sequence.
ACCESSION   DASPRR010000349 DASPRR010000000
VERSION     DASPRR010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208472"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VEL68_13140"
     CDS             complement(<1..765)
                     /locus_tag="VEL68_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36956.1"
                     /translation="MTLQQPTLPGRQGQINGGSRVLEPLDRRKENLMGQKDGYRQFID
                     WLNQTWWGLPPAAELLPLIQTCYTPEEADLLTGMPFSGRSLEELAAAKGMEPTALGSQ
                     IDRLAQKGFVFRNAAGQTVRYSLNDSFFVFLRSLFWGGKKDEKSRGLAPLVNQYYYHG
                     FFEQYSGVQTRGLRTVPIGKTVEDPRQVLPYEDVARFLDEQKYFTVSVCPCRHRKNLD
                     PNSPDCQHPTENCLHFGTLGRYAVENGMGREITKPEA"
     gene            complement(698..2083)
                     /locus_tag="VEL68_13145"
     CDS             complement(698..2083)
                     /locus_tag="VEL68_13145"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ36957.1"
                     /translation="MGQQEKISRRRVAKEPFREHLAMVVTAVLVGVVVLAVVNYFIEP
                     APPRTVIMTTGMEGGSYAIYAEWYRQILGREKVRLELLPSSGSVENLRRLRDPSFKVD
                     VGFVQGGTGSRSEAPNLFSLGALFYNPLWVFYRSPERYDDLSRLKGKKINIGPEGSGV
                     KKFAVDLLEASKAADASTSLLDLANPAAAKELVEGRIDAVMIFGTADSPTVQELLHKE
                     GVRLLSFSQAEAYTRLFPALSHVILPAGLLDLARKKPPADVHLLAVTTNLVARDTLHP
                     ALVYLLLDAASEVHSGGGWVHKAGEFPSPKAQDFPLSDRAERFYKSGRPLLFDYLPFW
                     VATFVDRLVLLLVPTAAVLIPLIRIMPWFYSWRNRRKFFYWYGELKDLELEVQESPKP
                     ERTGEYRTRLDKIEAEVNDVHVPLAFFHEAYDLKQHIELVRGKLVRLNDLTAADAARA
                     AGADQWRQSGS"
     gene            complement(2223..>2576)
                     /locus_tag="VEL68_13150"
     CDS             complement(2223..>2576)
                     /locus_tag="VEL68_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HXZ36958.1"
                     /translation="LHPSKKWSTWSPGRFFIPKEDGAFREAMIKGIKAYDRRPRMEGG
                     FPHVLMRSNLYLTCGNCQLLCWPDLADRKENFRVLTSTGVIIQRLDGSLHRVSPEEGE
                     TYMLTLDEEKRALYT"
BASE COUNT          516 a          775 c          723 g          562 t
ORIGIN      
        1 ggcctccggt ttggtgatct cccgccccat gccgttttcc acggcgtacc gcccgagagt
       61 gccgaaatgc aggcagtttt ctgtcgggtg ctggcagtcg ggggagttgg ggtccaggtt
      121 tttccggtgc ctgcagggac agacgctaac cgtaaaatac ttctgctcgt ccaggaaccg
      181 ggccacgtct tcgtagggga ggacctggcg ggggtcctcg acggtcttcc cgatgggcac
      241 cgttcgcaat cccctggttt gcactcccga atactgttcg aaaaacccgt ggtagtagta
      301 ctgattcacc aaaggggcca gtcctctgct tttttcgtcc ttcttaccgc cccaaaatag
      361 gctgcgcagg aagacaaaaa aggaatcatt caggctgtag cggaccgtct gtcccgcggc
      421 gttccggaag acaaatccct tttgggcgag tcggtctatt tgggaaccca gggcagtggg
      481 ttccatccct ttcgcggctg ccagctcctc aaggctcctc cctgaaaagg gcatgcccgt
      541 caggagatcg gcttcttccg gcgtgtagca ggtttgaatc aaggggagaa gttcggccgc
      601 cggggggagc ccccaccagg tctggttcag ccaatcgatg aactgtcggt acccatcctt
      661 ttggcccatg agattttctt tcctcctatc cagaggttca agaacccgac tgccgccatt
      721 gatctgcccc tgccgcccgg gcagcgtcgg ctgctgtaag gtcattcagc ctcacgagtt
      781 ttccccggac caattcgata tgctgcttca ggtcgtaggc ttcgtgaaaa aaagccagag
      841 gaacgtgaac atcgttcacc tcggcctcga tcttgtccag cctcgtccgg tactccccag
      901 tgcgctccgg tttcggactc tcctgcactt ccagttccag gtctttgagc tctccgtacc
      961 agtagaagaa tttccgccgg ttccgccagg agtagaacca aggcatgatt cggatgagag
     1021 ggattaaaac cgccgccgtc ggaaccaata gcaggaccag tcggtccacg aaggtggcaa
     1081 cccaaaaagg gaggtaatcg aacaacaacg gtcgcccgga tttgtaaaat cgctcggccc
     1141 ggtcgcttaa cggaaagtcc tgggccttgg gcgatggaaa ctctccagcc ttgtgtaccc
     1201 atcctccgcc gctgtgaacc tcggaggccg cgtccaggag tagatacacc agagccgggt
     1261 gaagggtatc gcgtgccacg aggttggtgg tgacggctag aaggtgcacg tccgccggcg
     1321 gcttttttct cgccaggtcc aacagccccg cgggtaagat gacgtgagaa agggccggga
     1381 aaaggcgggt ataggcctcg gcttggctga agcttagaag cctaaccccc tctttatgca
     1441 ggagttcctg caccgtggga ctgtccgccg tgccaaagat catgaccgca tcgattctcc
     1501 cttccacgag ctccttggcc gcggccgggt tggccaagtc cagcagagaa gtggatgcat
     1561 ccgcggcctt gctcgcctcc agcaggtcga cggcgaattt cttcaccccg ctcccctccg
     1621 gcccgatgtt gatcttcttc cctttgaggc gggagaggtc gtcgtacctt tcagggcttc
     1681 ggtagaacac ccagaggggg ttgtagaata aggcccccag ggaaaaaaga ttcggcgcct
     1741 ctgaccggga accggtcccg ccttggacaa aacccacgtc caccttaaag gatggatccc
     1801 ggagtcgtct caagttctca accgaacccg aagaggggag caattccagg cggactttct
     1861 cacggccgag gatttgccga taccactcgg catagatggc gtagctgcct ccttccatac
     1921 cggtggtcat aatgacggtc ctgggcgggg ccggctcgat aaaataattg accacggcga
     1981 ggacgaccac tcccaccagg accgcggtaa ccaccatcgc taggtgttcc cggaacggtt
     2041 ctttcgcgac ccttctgcgg ctgatctttt cctgttggcc catcgttccc tttactgggt
     2101 cctaaaggtt ttccctccgc aaggaaaacc ctctcacata ttctcccttt gtttgagaaa
     2161 aaagtttttc ttgccttccc acatctcccg gcccaaacct acattcctca aggcacacac
     2221 cattaggtgt aaagagcccg cttctcctcg tccagagtta gcatgtacgt ttccccttct
     2281 tcgggggaaa ccctgtggag ggacccgtcc agccgctgga tgatcacccc cgtggaggtc
     2341 aagactcgga agttttcctt tcggtcggcc aggtccggcc aacagaggag ttggcaattc
     2401 ccgcaggtga ggtacagatt ggaccgcatg agaacatggg ggaaaccccc ttccatcctg
     2461 ggccgtcgat cataggcctt gattcccttg atcatggcct cccggaaggc cccgtcttcc
     2521 ttagggatga agaatcggcc gggcgaccag gtggaccact tcttcgaggg atggag
//
LOCUS       DASPRR010000350        17607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1047121, whole genome shotgun sequence.
ACCESSION   DASPRR010000350 DASPRR010000000
VERSION     DASPRR010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 17607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17607)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17607
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047121"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="rsmB"
                     /locus_tag="VEL68_13155"
     CDS             complement(<1..1260)
                     /gene="rsmB"
                     /locus_tag="VEL68_13155"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HXZ36959.1"
                     /translation="MGNSLPYDPVRMLAWKILQEIERSSSFADLALDQAFFQTPDLRP
                     LDRAMISEMVMGILRWRGRLDAHLQQVLKSKDRKVDPRVLQLLRLGAYQILIMDRVPE
                     SAAVNESVRLAKALFKNENIAGFVNALLRSVIRNKTRDFFPPLAERPVDHIVQSFSHP
                     PWLVERWVKEFGPEVALRICAANNQKPPFTIRTNTLKITREALQRRLQEAGIPAQATL
                     HSPDGLTLPKTPDLMKDPLFQEGMYFIQDESSQMIPFLLEPQAGERILDACAAPGGKT
                     THLAQLMENRGEIFALDLMGSKLGLIQENCHRLGVTMVQVMEGDATQPLPFPEGTDFH
                     RVLVDAPCTGLGILHRNPEAKWRRKPEDIPRLAKVQAAILDQASSWLKPGGVLVYSTC
                     TMTPEENDGVVETFLARHPDFEREDLSK"
     gene            complement(1272..2204)
                     /gene="fmt"
                     /locus_tag="VEL68_13160"
     CDS             complement(1272..2204)
                     /gene="fmt"
                     /locus_tag="VEL68_13160"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXZ36960.1"
                     /translation="MKVLFLGTAEFACPSLEALLDSPHEVMGVLTQPDRPRGRGQKWI
                     PSPVKSIALKATLPIYQPEKVRDPDFLKTLESLRPEIIVVVAYGQILPAAVLAIPSRG
                     CLNVHGSLLPKYRGAAPIARAIQAGETHTGITTMFMDPGMDTGPMLLTERTEISEEDT
                     AGSLHDRLSRIGASLLLRTLEGLETNTLTPIPQDHSQATYAPKVEKEEANIRWSSAAR
                     QVFNLIRAFDPWPGAFTFWSGRTLKLFRPRLVEGEGQEPPGTVLQASGEGLLIATSDG
                     QLLVREVQLESRPRMGVVEFLRGNPLRSGVRLGE"
     gene            complement(2201..2716)
                     /gene="def"
                     /locus_tag="VEL68_13165"
     CDS             complement(2201..2716)
                     /gene="def"
                     /locus_tag="VEL68_13165"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXZ36961.1"
                     /translation="MAVLPILCFPNPVLKEKARPVQKIDDSIRKLVQDMAETMYAAPG
                     VGLAANQVGSLFRLAVIDATAANEPKNLLVLINPEIISAEGECTWDEGCLSVPECNEE
                     VKRNQKVVVRFLSLQGERVELEAEGLLAIALQHEIDHLDGFLFIDRISPLKRRLIKKK
                     LRKKENEGSPA"
     gene            complement(2723..5182)
                     /gene="priA"
                     /locus_tag="VEL68_13170"
     CDS             complement(2723..5182)
                     /gene="priA"
                     /locus_tag="VEL68_13170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXZ36962.1"
                     /translation="MSSTRQYIEVALPLPVRKTFTYEVPQDLGEAPSPGKRVLVPLGR
                     RLLTGFVLGPAPPLPAHTTILPIREVLEDDVSLSPKLLDFLLWTSAYYLQPLGEVIKT
                     SLPAGTQTRSKETIFLTPKGTEALEILKPESIEKQVLEELARKGGEGITSSSLTRKFS
                     TPIRSLLRKMQEKGWVGKKEDLEMPPVRGKKINWVRVRPFPEEISLTARQREVATFLQ
                     NAREIPVAELKARFKNASAVLTKLKAHGLAEIVPQEAFRQPSWEGLEDWIDGPPSLLT
                     EDQDRALREIVASLDSRKYQPFLLHGVTGSGKTEVYLRAIDQALSRGRQALLLVPEIS
                     LAGQLVAYFRARTPYPLAVLHSGLSAGERYDEWRKVKKGLARLVIGVRSAIFAPLESL
                     GIIIVDEEHDPSYKQDDKVRYHARDLALVRGKMEGAVVVLGSATPSLESYYNAREGKF
                     RLLSLPKRIDGRPLPEIQVLDMRLEQGEGRERSLFSLPLRKALEETVARGEQAFLFLN
                     RRGFSTFALCRDCGFTYRCPNCNVSLIYHLPDRTFRCHYCDHTLFAADRCPQCASSSL
                     LLFGSGTQRLEEEIKKMIPQIPVARMDRDTTTRKGSHQKILGQLRRGEVNLLVGTQMI
                     TKGHDLPRVTLVGVLAADLSLNLPDFRAGERTFQLLTQVAGRAGRSDLPGKVFIQSFN
                     PGHYSIQMAHAQDYTGFFEREVQFRRQMNYPPFVRLVNLRFEDNSEKRITRFAQALEG
                     LVQRKFKEDPKLGKHLEALGPAMAPLGKLKGRHRYQMLFKGKQWKSLHDFVEKIRAEM
                     EAEIPGRGVKLIVDVDPVHML"
     gene            complement(5345..7711)
                     /locus_tag="VEL68_13175"
     CDS             complement(5345..7711)
                     /locus_tag="VEL68_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXZ36963.1"
                     /translation="MNPFEVLPRVIEITNAPIEVDRRLKNLVDMLAHHFSLSLCALFL
                     WDSHQSRFSLKSISAKHPSFPPELSIPPQEGPLAACTVEKIPILIPDSSQLSLFSPPL
                     PPAFAEFRFLGFFPIFDDIFLYGVLTLLGDQPRELSGEEKETFLAIGRQLCGTLRSSQ
                     VSLQAKKRIAELSTLHAIGVAISSTLDLGELLRRISLSSAKILQGDGSLLHFLDEDSG
                     LMKLVSSYGIEEGDSPLTPLVLGEELVGTVALTGEPIALRETQTSHYSFRGLPPGICS
                     LVCVPLISQSRTIGTLTLFSFYQQGRQPKVFDEEDKNLLFTMASQVAVAIENSIVMHR
                     AETLAKEKERNLRELFLLYEISRAMLTTINLDRLLRIILISITLGSRLGFDRAALFLI
                     DEKENLLRGMMGVGPGSQEQADRWREKLESHSPFSPDWVIPEEMAETPYDNRVRRVRV
                     SLEETHSVLVKTLREGRSFNIEDALTDPLVNPEILRWFDSKAFASVPLMAKEKAIGLI
                     AVDNQLSGRPITETDIGFLNLLANQAALAIENSRLYSNLQEINTQLLNTQNRLIQSEK
                     LAALGEVVASIAHEIKNPLVSIGGFARRLERNLKDNSSEKKYMRIVLKEVNRLENVLN
                     QTLVFSRDLPLPSGHQDVNRVLEDSLSILEGEFQESHITVNRDLASNLPTLASDPQQL
                     KQVFLNLFVNAIQAMGSGGQLSVKTSLAPKEEKTWLQVEVEDTGGGIPQENLENIFNP
                     FFTTKQEGTGLGLAITHKIVTRYGGEIEVINRPGQGATFVIRFPLAKN"
     gene            complement(7708..9174)
                     /gene="glgA"
                     /locus_tag="VEL68_13180"
     CDS             complement(7708..9174)
                     /gene="glgA"
                     /locus_tag="VEL68_13180"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HXZ36964.1"
                     /translation="MKILFASPEAVPYAKTGGLADVSGALPMALAQMGHEVHLLLPKY
                     RAVDEKRFPAVQTGMVLKVPISQKVINAEVYALEPAPRLRVLLLRQDAYYDRDQLYGT
                     PSGDFEDNAERFIFFSRAVVEAALELDPDILHCNDWQTGLVPVYLKTIYRWSPALARA
                     ASIFTIHNIGYQGLFWHYDMHLTNLAWDLFTPQALEYYGKLNLLKGGIVFADAVTTVS
                     RKYMEEVQTPEYGAGLDGVLRDRKADLYGILNGVDYQEWSPAKDRFIKEKFDPSDLRG
                     KRSCKADLQQEFYLKVDPDIPLLGMISRLTDQKGFDILAPIMEPLLNLGVQFILLGTG
                     EEEYHLLFQKIGQKYPQKAGIRIAFDNTLAHKIEAGADMFLMPSLYEPCGLNQVYSLK
                     YGTVPIVRATGGLDDTIHDFNPLTEEGTGFKFEKYDSSCLLEAIKRALQVYKNRTVWE
                     KLMARGMAEDFSWEQAARDYVRVYEKALAKKKAPKAEP"
     gene            complement(9185..10183)
                     /gene="galT"
                     /locus_tag="VEL68_13185"
     CDS             complement(9185..10183)
                     /gene="galT"
                     /locus_tag="VEL68_13185"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HXZ36965.1"
                     /translation="MPELRKDPVTGRWVIISTERGKRPSDFGLQKVKPKEGFCPFCPG
                     NEDKTPPEILAFRQDGSHRNGPGWRLRVVSNKFPALRVEGDIFREGVGLYDKMNGIGA
                     HEVIIETPNHQESLATLAPKAFEEVLWSYRDRMLDLRRDTRLRYAMLFKNHGEAAGAT
                     LEHSHSQLIALPIVPHLVMEEMAGAREYYRYKERCIFCDIVRQEFQHGERVVLENGEF
                     IAIAPFASFSPFEVWILPKRHSSFFEESQVHEIQSLSWIFQEALKRLEKALNFPPYNF
                     TLHTTPFNERTLEYYHWHFEIIPKLTKFAGFEWGSGFFINPTPPEEAAKYLRELAP"
     gene            complement(10226..12379)
                     /locus_tag="VEL68_13190"
     CDS             complement(10226..12379)
                     /locus_tag="VEL68_13190"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HXZ36966.1"
                     /translation="MKAINLLFAVHNHQPVGNLEEVFRRSWEDGYDPFLKTLENHPGF
                     RISLHYSGSLLEWLEEYQPTFMTRIRGLVERGQVELLSGGFYEPLLPSIPERDAVGQV
                     RLSNHYLTEKFKVPPQGLWLAERVWSPTLPKVLAPSGLKYTLLDDSHFRYAGFPDEDI
                     FGYYVTESEGCTLSIFPSNKRLRYAIPFRSPEETLDILRRYATDSGHLAVTYADDGEK
                     FGIWPGTHRWVYQEKWLEKFIGALEENHDWVRLLTFSEYLEKFPPQGRAYLPPASYDE
                     MMEWALPPLSAIAFEDLLEELKRERRYEKYWPYLRGGIWENFLVKYPESNHMHKKMLR
                     VSQKVHRALQSKFISPKDFPPHPALQSLWKAQDSCAYWHGLFGGLHLNYLRHAVYQNL
                     IAAERLAEILERGGGKFFHQEILDLDKDLQPEVLVTTPDLGAILKPNYGGGLVELDYR
                     PKRFNLTNVLTRRPEAYHRKLKKSQTGVGGESLRPKNHEAEETLFYDWYTRSSFLDHF
                     LGEGATLDQFRRCQYPELGDFVNQPYTLTGIEESDGSGIMRISLLRKGSLWKREGRVP
                     VDTLKRFLFYREQPRMEVGYEITNRGPAEIVVWFGVEVNLTLLAGDDPQRYYLFPGLK
                     VDDRRLCSSGAIPEVEHLRLRDEVERFEVSLDLSPKGQLWRFPLDTVSQSESGIKKTF
                     QGTVLLFHWPFSLKPEETKSLGIQVVIEGIRPPGE"
     gene            complement(12555..14723)
                     /locus_tag="VEL68_13195"
     CDS             complement(12555..14723)
                     /locus_tag="VEL68_13195"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HXZ36967.1"
                     /translation="MLHVAFFWHMHQPYYRDPLTGEYSLPWVRLHALKGYYDMISLLT
                     DYPTIQQTFNLVPSLIRQLEDYGRGEAKDVFLEYSRRPAEDLTPEEKKFLLANFFMCY
                     WENMVKPYPRYRDLLRKRGVKVPEHRLDDLLKEFTTQEFRDLQVWFNLTWTGHRARKE
                     REVFRALLNKGRDFSEAEKASLLQAQQEIIQELIPFYQSLRLRGQVEITVSPFYHPIL
                     PLLMDWQYASRAMPRVALPGSFSHPEDAEAQIRRAVSFYEKTFGSRPEGMWPSEGSVC
                     PELVPLLQRAGIRWMATDEGILFRSLSEETSRLLLYRPYRVRFKSSEVSVIFRDRNLS
                     DLIGFTYSKNPPQSAADDLVAHLKNINHSLSQGQDGIILIALDGENPWEYYPDGGEAF
                     LTSLYERLSLESSLKTVRVGEYLESHPPRETLDSLHTGSWIDQNFRVWIGHPEDNLAW
                     NCLKRTRGFLEKAASKDTNLSPGKKEMAWEEVYIAEGSDWFWWFGDDFTSDNDEEFDR
                     LFRMHLSNVHLLLGMEVPDYLKIPISSPHEVKPTLEPLGLISPSLDGQVTHFYEWREA
                     GYFSAKTTGGSMYRGEGYLSGFYFGFDLERLYLRLDPIWRKAESLQGLDIHIHFSQPR
                     EWEIVFPLRFPSGKKAFYDLYARDGEGLSLKEHFTQIESDKIVELSIPFRVLQFSPRE
                     KAQFFIQVQRGDLAAERYPRNGFLSFFVPDQDFELVHWQI"
     gene            15004..15936
                     /gene="trxB"
                     /locus_tag="VEL68_13200"
     CDS             15004..15936
                     /gene="trxB"
                     /locus_tag="VEL68_13200"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXZ36968.1"
                     /translation="MGKIDYDLIIAGGGPAGLTAGLYAARAMLRTLLLEKMAPGGQAA
                     TTFFVENYPGFPEGISGPDLAQAMENQAKKFGLEVTYGEVKKMSFQEPLWEVEYEDRK
                     FLAKAVIVASGVESRKLGVPGEETLRGRGVSYCATCDGPFFKDQEIGVVGGGDSAVEE
                     ALYLTRFVSKIYLVHRRNALRAEKIIQERAFQNPKIEFLWDTVVTRILGDSGVEGLEL
                     QNVKTKALRTLKVGGVFLYVGSLSNTEFLKGIVKLDEQGYIVTDDQMATSAPGVFAAG
                     DVRKKLLRQIATAVGDGAMAAFAAGQYIESRLWP"
     gene            15949..16692
                     /gene="surE"
                     /locus_tag="VEL68_13205"
     CDS             15949..16692
                     /gene="surE"
                     /locus_tag="VEL68_13205"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXZ36969.1"
                     /translation="MRILVTNDDGIESEGLVALARALEKVAEVWVVAPDREQSAVSHA
                     LTMGRLLRKKRVRTLGPRYFAVNGTPTDCVLLGVNKILPERPSLIASGINKGENLGDD
                     ITYSGTVSAAIEGTILGIPSFAISLVSRNRFHFAHAAAFAVRLSSNLLSTGLPKNTFL
                     NVNVPGKQKPKSYRITRMGKRIYGDSVQERVDRWGRKYYTIGGNVPGYADTEDSDFKA
                     IARNLISITPLHLDWTNYASLETLLKRKI"
     gene            16747..17409
                     /locus_tag="VEL68_13210"
     CDS             16747..17409
                     /locus_tag="VEL68_13210"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXZ36970.1"
                     /translation="MDYNRAREKMVKEQIIGRAIQDRRVIEAMLRVPRHLFLPEGLLG
                     QAYGDTALPIGDGQTITQPYMVAFLSQALALQGTEKVLEVGTGSGYQAAVLACLADRV
                     YSVERIRSLLEQARKALDRVHCHNVITRLSDGSYGWKEEAPFDAILVTAGAPSLPRPL
                     ADQLKVGGTMVIPIGDRKGQRLTRIRRSSHGFFREDLMGCNFVSLVGEHGWAKKEKVS
                     GP"
     gene            complement(17396..>17607)
                     /locus_tag="VEL68_13215"
     CDS             complement(17396..>17607)
                     /locus_tag="VEL68_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-ethylammeline chlorohydrolase"
                     /protein_id="HXZ36971.1"
                     /translation="LNKPHLQPVYNIVSHLVYSATGADVRDVIIDGKVIMENRKLLTL
                     DEEEILRKAREWGEKIRGQSAIMGR"
BASE COUNT         3822 a         4714 c         5208 g         3863 t
ORIGIN      
        1 cttggatagg tcttccctct caaaatccgg gtgtctggcc aaaaaggttt cgaccacccc
       61 gtcgttttct tccggggtca tggtacaggt gctataaacc aggaccccgc ctggtttcaa
      121 ccaagaagac gcctgatcga ggatggctgc ctggacctta gccaggcggg ggatatcctc
      181 cggctttcgc ctccatttgg cctccgggtt ccggtgaaga attcccaatc cagtgcaggg
      241 ggcatcgaca aggacccgat ggaaatccgt tccttcggga aagggaaggg gctgagtcgc
      301 atccccttcc atgacttgga ccatggtaac ccccaagcga tgacaattct cttggatgag
      361 gccaagtttg ctgcccatga ggtccagggc gaaaatctcc ccgcgattct ccatgagctg
      421 ggccagatgg gtggtttttc cgccaggagc tgcgcacgcg tccaagatac gctctccggc
      481 ctggggttca aggaggaagg ggatcatctg agatgattca tcctgaatga agtacatccc
      541 ttcctggaat aaagggtcct tcatcaggtc cggggtcttg ggcagagtca acccatcggg
      601 agaatggaga gtagcttgag caggaattcc ggcctcttga agtcttcttt gtagggcttc
      661 ccgggtgatt ttcagagtat tcgtccggat ggtaaacggg ggtttctggt tgttggcggc
      721 gcaaatccga agagctacct ccgggccgaa ctccttgacc cagcgttcca ccagccaggg
      781 cgggtgggaa aaggattgaa cgatgtgatc gaccggcctt tccgccaagg gagggaagaa
      841 atctcgagtc ttattgcgga tcaccgaccg gaggagggca ttgacgaaac cggcaatatt
      901 ctcgttctta aagagagcct tggccaagcg aacgctttcg ttcaccgccg cggattccgg
      961 cacccggtcc atgatcagaa tctggtaggc ccccaaccgg agaagttgaa ggacgcgggg
     1021 gtcgactttt ctatccttgg atttcaagac ctgctgcagg tgcgcatcca gccttccgcg
     1081 ccaccgcagg attcccatga ccatctccga gatcatggcc cggtccaggg gtctcaggtc
     1141 gggggtttgg aaaaaagctt ggtcgagggc caaatccgca aaggaggaag aacgttcaat
     1201 ctcctgcagg attttccagg ccagcatgcg caccgggtcg taggggagtg aatttcccat
     1261 ggggagtccc attattctcc caggcgcact ccgctgcgaa ggggattacc ccggagaaat
     1321 tccactaccc ccatacgggg gcggctttcc agctgaactt cgcggacgag gagttggcca
     1381 tccgaggtgg cgatgaggag accctcgccc gacgcctgga ggaccgtccc tggaggctct
     1441 tggccctctc cttccaccag gcggggtcga aagagcttga gggtccgtcc cgaccagaag
     1501 gtaaaggctc cgggccaggg atcgaaagcg cggatcagat tgaagacctg ccgggcggca
     1561 gaactccacc ggatattggc ttcctccttt tcgactttcg gagcataggt agcctgggaa
     1621 tgatcttggg ggataggggt gagggtattc gtttcaagtc cctccagggt gcgtaggagc
     1681 agactggccc cgattcggga aaggcgatcg tgcaagcttc ccgcagtgtc ttcctcactg
     1741 atttccgtcc tttcggtcaa aagcatggga ccggtgtcca ttcccgggtc cataaacatg
     1801 gtggtgatcc cggtatgggt ctctcccgcc tggatggccc gggcgatggg ggcggcccct
     1861 cgatatttgg gcagaaggga accgtgaaca ttcaagcacc ccctggaagg aatggccaac
     1921 accgcggcag gaagaatctg cccgtaggcc actacaacga tgatctcggg ccggagggat
     1981 tccagggtct ttaagaaatc cgggtcacgg accttttcgg gctggtagat ggggagggtg
     2041 gccttcaaag cgatcgactt gaccggagaa ggaatccatt tttgtccccg ccctctgggg
     2101 cgatccggct gggtaagaac tcccatcact tcatggggag agtccaggag ggcttccagc
     2161 gagggacagg cgaattccgc agtacccaga aagaggacct tcaagcggga cttccttcat
     2221 tttctttctt tcggagcttt ttctttataa gccttctctt gagggggctg atccggtcga
     2281 tgaagaggaa cccatccaaa tgatcgatct cgtgctgcaa ggcgatggcc aaaaggccct
     2341 ccgcctccaa ttccactctt tctccctgga ggctgaggaa tcgaacgacc accttttggt
     2401 tccgtttcac ttcttcattg cattccggca cgctcaggca gccttcatcc caggtgcatt
     2461 ccccctcggc agagatgatc tcggggttga taagaaccaa aagatttttt ggctcattgg
     2521 cggcagtcgc gtcgatcacc gccaggcgaa agagagaacc cacctgattc gcggccaggc
     2581 cgactcccgg ggcggcgtac atggtctcgg ccatgtcttg aaccagcttg cggatggaat
     2641 cgtcgatctt ctgaaccggc ctggcttttt ccttcagaac cggattggga aaacacagaa
     2701 tgggaagaac cgccatattt ttttatagca tatgcaccgg gtccacatca acgatcaatt
     2761 ttactccccg tccgggtatc tctgcttcca tttcagccct aatcttttcc acgaaatcgt
     2821 ggagggactt ccattgtttg cccttgaaga gcatttggta gcggtgcctt cccttcaatt
     2881 ttcccagagg agccatggcc gggccgaggg cctccagatg cttcccgagt ttcgggtcct
     2941 ccttgaattt cctctgaacc agcccttcca gggcttgggc gaaccgggtg atccgttttt
     3001 cggaattgtc ttcaaaccgg aggttgacca aacggacaaa gggcgggtag ttcatctgcc
     3061 ggcgaaactg gacctcccgc tcgaagaagc cggtgtagtc ctgcgcgtgg gccatctgaa
     3121 tgctgtagtg gccgggattg aaggactgaa taaacacttt cccgggcaag tcgctccgtc
     3181 ccgccctacc ggcgacctgg gtgagaagtt ggaacgtgcg ctcgccggca cgaaaatcgg
     3241 ggaggttcag ggagaggtct gcagccaaaa ctccgacaag ggtaacccgg gggaggtcgt
     3301 gccctttggt gatcatctgg gttcccacca ggagattcac ctctccccgg cgcagctggc
     3361 ccaggatctt ctggtgggag cccttgcggg tggtcgtgtc ccggtccata cgggcaactg
     3421 gaatctgggg gatcattttc ttgatctctt cctccaggcg ctgcgttccg cttccgaaga
     3481 ggagaaggct ggaggaggcg cattgcgggc agcgatcggc ggcgaatagg gtgtggtcgc
     3541 agtaatggca gcgaaaggtc ctgtccggca agtgatagat caaggagacg ttgcaattgg
     3601 ggcaacggta ggtgaaaccg caatcccggc aaagagcaaa agtggaaaat cccctccggt
     3661 tcaggaagag gaaagcctgc tcccctctcg ctacggtttc ctccaaggcc ttccgcaaag
     3721 gcagggagaa gagggacctc tcccttcctt ccccctgttc caggcgcatg tccaggactt
     3781 gaatctcggg gagaggtcgc ccgtcgattc gcttggggag cgataagagt cgaaactttc
     3841 cttcccgggc attatagtag gactccaaag aaggggtggc ggagcccaga acgaccacgg
     3901 cgccctccat tttcccccga accagagcta aatcccgggc atggtagcga accttatcgt
     3961 cctgcttgta agaagggtca tgctcttcat ccacgataat gatgcccagg gattccaggg
     4021 gggcgaagat ggccgatcgg actccaatca cgagtctggc cagccccttc ttcacctttc
     4081 gccactcatc ataacgctct ccagcggaaa ggccgctgtg gagaacggcc aggggatatg
     4141 gagtacgcgc ccgaaagtag gccaccagct gcccggcgag ggaaatctcc ggaaccagga
     4201 ggagagcctg ccgtccgcgg gaaagcgcct ggtcgatggc tcggaggtat acctcggtct
     4261 ttccgctccc tgtaacccca tgaagcagga agggctgata cttgcgggag tcgagggagg
     4321 cgacgatttc ccgcagagcc cgatcctgat cttcggtgag cagtgaaggg ggcccgtcaa
     4381 tccaatcctc cagtccctcc caagagggct gacgaaaggc ctcttgggga acgatttccg
     4441 ccagcccatg ggccttcaat ttggtcagca ccgcagaggc gtttttaaaa cgggccttca
     4501 attcggcgac aggaatttcc cgggcgtttt ggaggaaggt ggctacctct ctttgccttg
     4561 ccgtgaggga aatctcctcg gggaagggtc gaacccgcac ccagtttatt ttctttcccc
     4621 tcaccggcgg catttccagg tcctcttttt ttcccaccca acctttttcc tgcatctttc
     4681 gaagtaggct ccggatgggt gtggagaatt ttctcgtcaa ggaagaggaa gtaatccctt
     4741 cccccccctt tcgggccaat tcctctagga cttgcttctc aatagactca ggttttagaa
     4801 tttcgagggc ctcggtccct tttggggtca ggaagatggt ttccttggaa cgggtttgcg
     4861 ttcccgctgg aagggaggtt tttatgacct cgcccagagg ctggaggtag taggctgagg
     4921 tccagagaag aaaatccaac agtttcgggg aaagagaaac gtcatcttcc aacacctcgc
     4981 ggatgggaag gatcgtggta tgagcgggaa ggggcggggc cggaccgagg acaaagccgg
     5041 ttaggagtcg ccgccccagc ggaaccaaga cccgtttgcc cggggatgga gcttccccca
     5101 ggtcctgggg gacttcatag gtaaaagtct tacgaacagg cagggggagg gcaacttcaa
     5161 tgtattgtct ggtagaggac attgaaaagc aaatttcccg ctaaaatcaa ttttgttgat
     5221 ctcgttaaaa atcttctgaa gcgtcacccc ggcgaaagcc ggggtccaga atttctttaa
     5281 ataattggat tccggctttc gccggaatga cgaattttat cgaatttcca cacttacgag
     5341 atcatcaatt tttggccagt ggaaagcgga tgacgaaggt ggctccctgg cccgggcggt
     5401 tgatcacttc aatttctcct ccataccggg tgacgatctt atgggtgatg gccagaccca
     5461 ggccagtccc ttcttgtttg gtagtgaaga aggggttgaa aatattttcc aggttttcct
     5521 gggggatgcc tccccctgtg tcctccacct cgacctgcag ccaggtcttt tcctcctttg
     5581 gggcgaggga ggtcttgacc gaaagttgcc ccccgcttcc catggcctgg atggcgttga
     5641 cgaagagatt gagaaagacc tgcttcaact gctgggggtc gctggccaag gtgggcaggt
     5701 tggaagccag atcgcggttg accgttatat ggctttcctg aaattccccc tccaggatgg
     5761 acaaggagtc ctcgaggacc cggttcacat cctgatggcc ggaagggagg gggaggtccc
     5821 gggaaaagac cagggtctgg ttgaggacgt tctccaaccg attcacttcc ttgaggacga
     5881 tgcgcatgta cttcttttcg gaagagttat ctttcaggtt ccgttccagt cggcgggcaa
     5941 atcccccgat ggagaccagc ggatttttga tttcgtgggc gatggaggcc accacttctc
     6001 ccagggcggc cagcttttcg gattgaatca ggcggttctg ggtattcaag agctgggtat
     6061 taatctcctg gaggttgctg tacagtcggg agttttcgat ggccagggcg gcctgattgg
     6121 ccagcaaatt caggaatccg atgtccgttt cggtgatcgg tcttcctgaa agctgattgt
     6181 ccacggcaat gaggccaatg gccttttcct tggccatgag aggaaccgaa gcaaaggctt
     6241 tgctgtcgaa ccagcgcagg atttcggggt tcaccaaggg gtcggtgagg gcgtcttcga
     6301 tgttgaagga ccgtccttcc cgaagggtct tgaccaggac ggagtgggtt tcctccaaag
     6361 agacccgaac ccgccggacg cggttatcat aaggggtttc ggccatctcc tcggggatca
     6421 cccagtcggg agagaagggg gagtggcttt ccagcttttc ccgccaccga tccgcctgtt
     6481 cctggcttcc cgggccgacg cccatcattc cccgcaggag gttctccttc tcatcgatca
     6541 ggaagagggc cgcccgatcg aaccccagcc ggctccccag ggtgatcgag atgagaatga
     6601 tgcgcagcaa ccgatccagg ttgatggtgg tcagcatggc ccgggagatt tcgtagagga
     6661 gaaagagctc ccgcaggttc cgttcttttt ccttggccag ggtctccgcc cggtgcatga
     6721 cgatggaatt ctcgatggcc acagctacct gggaagccat ggtgaaaagg aggtttttgt
     6781 cctcttcgtc aaacaccttt ggttgcctac cttgctgata gaaactgaaa agggtaaggg
     6841 tgccgatggt cctcgattga gagatgagag ggacgcagac gagcgaacag atgcccggcg
     6901 gaagcccccg gaaggagtaa tgagaggttt gcgtctcccg cagggcaatc ggttcaccgg
     6961 tcagggccac ggtgcccacg agttcttccc ccaggaccag gggagtcaat gggctgtccc
     7021 cttcctcgat tccgtaggaa gagacgagct tcatcagccc ggagtcttcg tcgaggaaat
     7081 ggagaaggga gccgtccccc tggagaatct tggcgctgct gagagagatt cgacggagca
     7141 attcgcccag gtccaaggtg gagctgatgg ccactccgat ggcatgaagg gtgctcaact
     7201 cggcgattcg cttcttagct tggagggaaa cctggctgct tcgcaaggtg ccgcagagct
     7261 gcctcccgat ggctagaaag gtctcctttt cctcccccga gagctcgcgg ggctgatccc
     7321 ccaggagcgt caatacgccg tacagaaaga tgtcatcgaa gatgggaaag aatcccagaa
     7381 agcggaattc ggcgaaagcc gggggaaggg ggggagaaaa cagggaaagc tgggagctgt
     7441 cggggatcag aatgggaatt ttttccacgg tgcaggctgc cagagggcct tcctgcggag
     7501 gaatgctcag ttccggggga aaagagggat gcttcgcgct tatggatttt aaggaaaagc
     7561 ggctctggtg ggagtcccac agaaagaggg cgcagagcga taaggagaaa tggtgggcca
     7621 gcatgtccac caggttcttc agccgccggt ccacttctat cggggcgttg gttatttcaa
     7681 tgaccctggg caggacttcg aaggggttca tggctcggcc ttaggtgcct ttttcttggc
     7741 tagggccttt tcatagaccc ggacgtaatc gcgggccgcc tgttcccagg aaaaatcctc
     7801 ggccatgccc cgggccatta acttttccca gaccgtgcgg ttcttgtaaa cctgaagggc
     7861 tcttttaatg gcctccagaa ggcaggaaga atcgtatttc tcgaatttga acccggttcc
     7921 ttcctcggtg agggggttaa aatcgtggat ggtgtcgtcc aaccctcccg tggccctgac
     7981 gatagggaca gttccatact tcaaactgta gacctgattc aagccgcagg gctcatagag
     8041 ggagggcatg agaaacatgt ccgccccggc ctcgattttg tgagccagag tattgtcgaa
     8101 agcgatccga atccccgctt tttggggata cttctgtccg attttctgaa atagaagatg
     8161 gtactcttcc tctcccgtcc ccaaaaggat aaactggact cccaagttca ggaggggttc
     8221 catgatagga gccagaatgt cgaacccctt ctgatcggtc agccgggaga tcatccccag
     8281 aagggggatg tcggggtcca ccttcaggta aaattcctgt tggagatcgg ccttgcagga
     8341 ccttttcccc cttaggtcgg aaggatcgaa tttttccttg atgaagcggt ctttggccgg
     8401 cgaccactct tggtagtcca ccccgttcag gatcccatag aggtctgctt tccgatcccg
     8461 taaaacccca tccagcccgg ctccgtattc aggggtctgg acctcctcca tgtacttccg
     8521 gctcaccgtg gtcacagcgt cagcgaagac gattccccct ttcagcaggt tcagcttccc
     8581 gtaatactcc agggcctgag gggtgaacaa atcccaagcc aggttggtga ggtgcatgtc
     8641 gtaatgccaa aagagaccct ggtagccgat attgtggatt gtgaatatcg aggccgcgcg
     8701 ggccagagcg ggggaccagc ggtagatggt tttgaggtac acgggaacga gcccggtctg
     8761 ccagtcgttg cagtgaagga tatcgggatc aagctccagg gctgcctcta cgacagcccg
     8821 ggagaagaag atgaagcgtt cggcattatc ttcgaaatcc ccgctggggg ttccgtacag
     8881 ctggtcgcgg tcatagtagg catcctggcg gagaaggaga acccggagcc ggggagcggg
     8941 ctccagggcg tagacctcgg cgttgatcac cttctgggaa atgggaacct tcaggaccat
     9001 cccggtctgc accgcaggaa agcgcttttc atcgaccgcc cggtacttgg gaagaaggag
     9061 atggacctca tgtcccatct gggccaaagc catgggcagg gcgccggaaa catccgccag
     9121 gccgccggtc ttggcatagg gaacggcttc gggggacgca aacaggatct tcattttctc
     9181 ctcctcacgg ggccagctct cgcaggtatt tggccgcctc ttcagggggc gtaggattga
     9241 tgaagaaccc cgagccccat tcgaaaccgg caaacttggt caacttgggg ataatctcaa
     9301 agtgccagtg atagtactcc agggtccttt cattgaacgg ggtggtgtgc agggtaaagt
     9361 tgtaaggggg aaaatttaag gccttctcca gccgcttcag ggcttcctgg aagatccagg
     9421 agagggactg gatttcgtgt acctgggact cctcaaaaaa ggagctgtgc cgtttgggaa
     9481 gaatccagac ctcaaagggg gagaaagaag cgaagggggc tatggcgatg aactcgccgt
     9541 tttccagaac gacccgctcc ccatgctgga attcctggcg gacgatgtca cagaagatgc
     9601 accgttcctt ataccggtaa tattcccggg cgccggccat ctcttccata accaagtgag
     9661 gaacgatggg gagggcgatg agctgggaat gggagtgctc cagtgtcgcc cccgctgcct
     9721 ccccgtggtt tttaaagagc atggcgtaac ggaggcgggt gtctcttctc aaatccagca
     9781 tgcggtcccg gtaggaccag aggacctctt caaaggcttt cggagccagg gtagccaggc
     9841 tttcctggtg gttgggtgtt tcgatgatca cttcgtgggc tccaatccca ttcattttat
     9901 cgtaaagacc gaccccttcc cggaagatat ccccttccac ccgtaatgcc ggaaatttat
     9961 tggagaccac ccgaagccgc cagccgggtc cgttccgatg gcttccatcc tgccggaagg
    10021 ccaaaatctc ggggggggtc ttgtcctcgt tccccggaca gaagggacag aatccttctt
    10081 tgggcttcac cttctggagg ccgaaatcgg atggccgctt tcccctttcg gtggaaatga
    10141 tcacccaccg tccggtgacc gggtctttac gcaattcggg catgtattcc tcctaaaaat
    10201 tggccaaggt taaaaccagg ttatctcatt ccccgggagg gcggattcct tcgatgacca
    10261 cctggattcc cagggacttg gtctcttcgg gtttcaagga aaagggccag tggaagagaa
    10321 ggaccgtccc ttggaaagtc ttcttaatac cgctttccga ctgggaaacc gtatccagcg
    10381 gaaaacgcca gagctgtccc ttgggagaaa ggtccaggga gacttcgaaa cgctctactt
    10441 cgtcgcgcag tcgaagatgc tccacttcgg gaatggctcc gctggaacag aggcgccggt
    10501 catcgacttt caaccccgga aaaagatagt acctttgcgg gtcatctccc gcgagaaggg
    10561 tgagatttac ttcgaccccg aaccatacaa cgatctccgc cggtccccgg ttggtgattt
    10621 cgtaccccac ctccatgcgg ggttgttctc ggtagaagag gaatcgctta agcgtatcga
    10681 ccgggactcg cccctcccgt ttccagagac tgcccttccg taggagagaa atgcgcatga
    10741 ttcccgaacc gtccgattcc tcgattcctg tcaaggtgta aggctgattg acgaaatctc
    10801 ccagttccgg atattggcag cggcgaaact gatcgagggt ggccccttcc cccaagaaat
    10861 gatcgagaaa ggaagagcgg gtgtaccagt catagaaaag ggtctcctcc gcttcatggt
    10921 tcttcggccg aagggactcc ccccctacgc cggtctggct tttcttcagt ttccggtgat
    10981 aggcttcagg cctgcgggtt aagacgttgg tgaggttgaa gcgtttgggc cggtagtcca
    11041 gctcgaccag tcccccccca taattgggct tgaggatggc ccccaggtcc ggagtggtaa
    11101 ccaggacctc cggctgaagg tccttatcca aatcgagaat ttcctggtgg aagaattttc
    11161 cacccccccg ttccaggatc tcggccaggc gttccgcagc gatgaggttt tggtatactg
    11221 catggcgcag gtaattcagg tggagcccgc cgaataggcc atgccagtaa gcgcagctgt
    11281 cctgggcttt ccatagagac tggagagcgg ggtgaggggg gaaatctttg ggggagatga
    11341 atttgctctg cagggcacga tggactttct ggctgacccg aagcattttt ttatgcatat
    11401 ggttgctctc ggggtatttt accaagaaat tttcccagat cccgccccga aggtagggcc
    11461 agtatttttc ataccgcctc tcccgtttca gttcttccag caggtcttcg aaggcgatgg
    11521 ccgacagggg cggcaaggcc cattccatca tttcgtcata agaagcgggc ggaaggtagg
    11581 ctctcccttg gggaggaaat ttttccaaat actcgctgaa agtcaacaga cgaacccaat
    11641 cgtggttctc ttccagggcc ccaatgaatt tctccaacca tttttcctgg taaacccagc
    11701 gatgggtccc cggccagatt ccgaattttt ccccgtcgtc ggcatacgtc acggccaggt
    11761 ggccggagtc ggtagcgtac cgccgcagga tgtccagggt ctcttcgggg gagcggaagg
    11821 ggatggcgta acgcagccgt ttgttgctgg ggaagatgga aagggtacat ccctcgcttt
    11881 cggtgacgta atatccgaag atgtcctcat cgggaaaacc cgcatagcga aagtgggagt
    11941 catccaagag ggtgtacttc agacccgagg gggccagaac cttgggcagg gtagggctcc
    12001 agaccctttc ggccagccag aggccctgag gagggacttt gaacttctca gtcaggtaat
    12061 gattggaaag gcggacttgc ccgacggcgt ccctctcggg gatggaaggg agaagaggct
    12121 cataaaaacc accgctcaat agttcaacct gacccctctc caccaacccc cggattcggg
    12181 tcatgaaggt cggctggtat tcctccagcc attccagaag tgatccgctg tagtggaggg
    12241 agatacggaa tccggggtga ttttccaggg tcttgaggaa ggggtcgtac ccatcctccc
    12301 agcttcgacg gaagacttcc tccaggttgc ccacgggctg gtggttgtgc acggcaaaaa
    12361 ggagattaat cgccttcatg gaaacccatc cccctcaaaa agtgttgaaa caagggataa
    12421 ctggaatcag ggataatcgt gagtcatcgc cgagaggcat tttcccatga ttccataact
    12481 tccatttccc caagggtccc ccattcccag ctttccttca tcccttccaa aattcttccg
    12541 ccatccttga gtcttcagat ctgccaatga accaattcaa aatcctggtc cggtacgaag
    12601 aaagaaagaa acccgttgcg gggataccgt tccgccgcca ggtcccccct ttgcacctgg
    12661 atgaaaaact gagccttttc tcgcggggaa aattgaagca cccggaaggg aatggaaagc
    12721 tccacaatct tgtctgactc aatctgggtg aaatgctctt tgagggagag accctcgcca
    12781 tcccgagcgt agagatcgta gaaggctttt ttcccggagg gaaacctgag ggggaagacg
    12841 atttcccatt cccggggctg ggagaaatgg atatggatat ccaatccctg gagggattcc
    12901 gcttttctcc agatggggtc aagtcgcagg tagagccgtt ccaggtcgaa cccgaagtag
    12961 aaacctgaaa gatatccctc tccccggtac atggaaccgc cggtggtttt ggccgagaaa
    13021 taccccgctt cccgccattc gtaaaaatga gtgacctggc cgtccaagga aggggagatg
    13081 agccccaggg gttccagggt gggtttcact tcgtggggag aggaaatggg aattttcagg
    13141 taatcgggga cttccatgcc cagaaggaga tgcacgttgc tcagatgcat tcggaagaga
    13201 cggtcgaact cttcatcgtt gtcgctagtg aaatcatccc cgaaccacca aaaccaatcg
    13261 ctcccttcgg caatgtagac ctcctcccag gccatctctt ttttccccgg ggagagattc
    13321 gtatccttgg aagcggcctt ctccagaaat ccccgggttc gcttcaggca attccaagcc
    13381 aggttgtctt caggatgtcc gatccagacc cggaagtttt gatcgatcca ggaacctgtg
    13441 tggagggaat ccagggtctc ccggggaggg tgggattcca aatattcccc cacccgtacg
    13501 gtctttaagg aagattccag ggagagcctt tcatagagac tggtcaagaa tgcctctcct
    13561 ccgtcgggat aatattccca agggttttct ccgtcgaggg cgatcaggat aatcccgtct
    13621 tgcccctgag atagggagtg gttgatgttt ttcaggtggg ccaccaaatc atccgcggcg
    13681 gactggggag ggttcttgga atacgtaaaa ccgatgagat cggagaggtt ccggtcgcga
    13741 aagattaccg acacttcaga gctcttaaac cggacccggt aggggcggta aaggagcaat
    13801 cgactggtct cttcggagag ggatcgaaag agaatccctt catcggtagc catccagcgg
    13861 atgccggcac gttgcagaag gggaaccagc tccgggcaca ccgacccctc tgacggccac
    13921 attccctcgg gtcgactgcc aaaggttttt tcatagaaac tgaccgctct tcggatttga
    13981 gcctccgcat cttcgggatg ggaaaaagac cccgggaggg ccacccgcgg catggcccga
    14041 gaggcatact gccaatccat cagaagggga aggatgggat ggtaaaaagg gctcaccgtg
    14101 atttccacct ggccccggag gcggagcgat tgatagaagg ggatcagttc ctgaatgatc
    14161 tcctgctggg cctgcagcaa ggaggccttc tcggcctcgg aaaaatccct tcctttattc
    14221 agaagggcgc gaaagacttc cctctccttt ctggcccgat ggccggtcca ggtgaggttg
    14281 aaccacacct ggaggtcccg gaattcctgg gtggtgaatt ccttgaggag atcatccaag
    14341 cggtgctcgg gaactttgac tcctcgtttt ctaagaaggt cccggtaccg cggatagggt
    14401 ttaaccatat tctcccagta gcacatgaaa aaattggcca ggaggaattt tttctcctcg
    14461 ggggtcaaat cttcagcggg cctgcgggag tactccagga agacatcctt ggcttccccc
    14521 cgcccgtagt cctccagctg gcggatcagc gaagggacca ggttgaaagt ctgctggata
    14581 gttgggtagt cggtcagcag ggagatcata tcgtaatagc ccttgagagc atggagccga
    14641 acccagggga ggctgtactc cccggtcaga ggatcccggt aataaggctg gtgcatgtgc
    14701 caaaaaaacg cgacgtgaag catatccctc cgataatcaa ggatgattat aacaaatccc
    14761 gaacaggaaa caataaaaac aactctccgc ttacaggtta ctagtttcaa atacagggtg
    14821 ccaaactttg ggcttcagct tcctgattgg cgaatcgcaa tttgcgcttg agacccgaaa
    14881 cccgaggtct gaacctgcaa cttcggactt ttggcttgtg cccggagggc aacttgaaac
    14941 tggttttttc cgggcattgt gtgctatttt tattcggtag attcctgaat gcggagccaa
    15001 gaaatgggca aaatcgacta tgacttgatt attgccggcg gaggccccgc cggtctcacc
    15061 gctggactgt atgcggcacg ggccatgctt cgaaccctcc tcctggaaaa gatggccccc
    15121 ggaggacaag ctgccacgac cttcttcgtc gagaattatc ctgggttccc ggaaggaatt
    15181 tccgggcccg atttggctca agccatggag aaccaggcga aaaaattcgg cctggaggtt
    15241 acctatggag aagtgaaaaa gatgtctttc caggaaccgc tatgggaggt ggaatacgaa
    15301 gaccggaagt tcctggccaa agcggtcatc gtggccagcg gggttgagtc gcggaaactg
    15361 ggggtcccgg gggaggagac ccttcgggga agaggggtgt cctactgcgc cacctgcgac
    15421 ggacccttct tcaaagacca ggaaatcgga gtggtgggag gaggggattc ggccgtggag
    15481 gaagccctct atctcacccg gtttgtgtcc aaaatctatc tggtccatcg ccgcaacgcc
    15541 ctccgggcgg agaagatcat ccaggagagg gccttccaga atcccaagat tgagttcctc
    15601 tgggacacgg tagtaaccag gatcctgggg gattcgggag tggaagggct ggaattgcag
    15661 aatgtaaaga ccaaagccct ccggacgctg aaggtggggg gggtcttctt gtacgtgggc
    15721 tcgctctcca atacagaatt cttaaaaggg attgtgaaat tggacgaaca gggttatatc
    15781 gtgaccgatg accagatggc cacctctgcg ccgggggtct ttgccgccgg ggatgtgagg
    15841 aaaaaactcc ttcgccagat tgctacggca gtaggggatg gggcgatggc cgcttttgcc
    15901 gcaggacaat acatcgagtc tcgcctctgg ccgtgagggg aaatcgggtt gaggatcctg
    15961 gtaaccaacg atgacggaat cgaatcggaa gggcttgtgg ctctggcccg ggcgctggag
    16021 aaggtggccg aggtttgggt ggtcgctccg gaccgggagc agagtgccgt aagtcacgcc
    16081 ttgaccatgg ggcgtctgct ccgaaaaaag cgggttcgga ccctgggacc ccgctatttt
    16141 gcggtcaatg gaacaccaac cgactgcgtt ctcctgggag tgaacaagat cctgcccgaa
    16201 cggcccagtt tgatcgcctc gggcatcaac aaaggggaga acctggggga cgacatcact
    16261 tactcaggca cggtttctgc agccatcgaa gggaccattc taggaattcc ctcctttgcc
    16321 atctccctgg tttccaggaa ccgttttcat tttgcccatg ccgccgcttt tgccgtgcgt
    16381 ctctccagca acctgctgag caccggcctt cccaagaaca cctttttgaa tgtcaacgtt
    16441 ccggggaagc agaaacctaa atcgtacagg atcacccgca tggggaagag gatttacggg
    16501 gactcggtgc aggaaagggt ggatcgctgg gggagaaaat actataccat tggaggcaac
    16561 gttccggggt acgcggacac ggaggactcg gatttcaagg ccatcgcccg gaatctcatc
    16621 tccattaccc cgctgcacct ggattggacc aactacgcgt ccctggagac gctcctgaaa
    16681 agaaagatct gaggttcatt ctttccggcg atgatgcggt ccaacaaggt ggatcagaca
    16741 aggtctctgg attacaaccg ggcccgggaa aagatggtga aggaacagat catcggccgg
    16801 gcgattcagg accgcagggt gatcgaggcc atgctgaggg ttccccggca tctcttcctc
    16861 ccggaaggac ttctcggaca ggcctatgga gacacggctc tacccattgg agacggacag
    16921 accatcactc aaccttacat ggtagctttc ctgagccagg ccctggcgct tcaagggact
    16981 gaaaaagtcc tggaagtggg gaccgggtcc ggataccagg cggcggtgct ggcttgcctg
    17041 gccgatcggg tctattcggt ggaacggatt cgttctctcc tcgagcaggc tcgaaaggct
    17101 ctcgatcggg tccattgtca caatgtgatc acccggcttt cggatggcag ctatggttgg
    17161 aaagaagagg cacccttcga tgccatcctg gtcaccgcag gcgccccgtc ccttccccga
    17221 ccgctggccg atcaactgaa agtcgggggt acgatggtca ttcccattgg ggaccggaag
    17281 ggccagaggt taacccgcat ccgccggagt tcccatggtt ttttcagaga ggacctgatg
    17341 ggatgcaatt tcgtttctct ggtgggcgaa cacgggtggg ccaagaagga gaaggttagc
    17401 ggcccataat tgcggattgc ccccttattt tctcccccca ttcccttgct tttctcaaga
    17461 tctcttcctc atccagggta agaagctttc ggttttccat gatcaccttg ccgtcgatga
    17521 tcacatcgcg cacatcggct ccggtggccg agtagaccag gtgggagacg atgttgtaga
    17581 ccggctgaag gtggggcttg ttcaagt
//
LOCUS       DASPRR010000351         8163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_399418, whole genome shotgun sequence.
ACCESSION   DASPRR010000351 DASPRR010000000
VERSION     DASPRR010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399418"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VEL68_13220"
     CDS             <1..1638
                     /locus_tag="VEL68_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXZ36972.1"
                     /translation="QEWGAKAVLQICHGGRCTSARYMGCQPIAPSAMPSYTGEMAREM
                     TLEEIEATMDSFAEAARRAKTAGFDGVELHGTHGYLLAQFLSPYTNRREDVYGKDRGL
                     FALQTLERVRARVGSDYLVGYRMSGEEFIDGGVTLQETKAFARRLEARGIDYLHVSGG
                     IIEVGEHFVIPMYFPKGHLLHLAEQIKQAVKIPVVAVGAIHDPSLAEESLKRKRADLI
                     AMGRGLIADPELPRKVQQNRPEDIRTCLRCNEGCISRVRQGKTQRCAVNAEVGRERTL
                     RIHPASRPKQVCVIGGGPAGMEAARVLAERRHKVTLIEKENELGGLLRFASVPDFKEE
                     LRGFLQYLKTQVKKTGVEVLMGRKASLDLVRDFHPDSVVLAAGSTMPIPGIPGVGRPF
                     VAGALDLLSGKFQPGDRVVVVGGAAMGCEVAAHLASLGRKVTVVEMINGLALDLENRC
                     RVALLQLLKERSVDILTNWKMEAIGDRMVLLSDGNRNKKEVTADSVILTLGLTPNQEL
                     LEPLTRNFPEVQVIGDCLEPRKVYQAIHEGAFAGRAI"
     gene            1762..2709
                     /locus_tag="VEL68_13225"
     CDS             1762..2709
                     /locus_tag="VEL68_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ36973.1"
                     /translation="MSEIRNVAIIGAGTMGHSLAQVFAQGGYNVWLNDVKDEIVARAK
                     KLIASNLDTLIELGNVEKSQKEPILRRIHTTVKLEEAGQNADFVIEAIIEDQAAKKEI
                     FSRLDKTCPSGAILASNTSYMDIFKFVETKRPDKVLITHWFAPPHIVPLVEIVRGPRT
                     SQEVVDRVKALMIQVGKKPIVITKFLPGFIANRLQSALGNEVFNLLDQGYASPEDIDT
                     ATKASFALRMPILGLVKRMDFAGLDLTQKILSNQTYKVPPQNTKSKTVERLVSQGKLG
                     VKTGSGYYEYGGRSTEEIMKERDMKLIKLREFLKEMGEL"
     gene            2820..4193
                     /locus_tag="VEL68_13230"
     CDS             2820..4193
                     /locus_tag="VEL68_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXZ36974.1"
                     /translation="MFVDSHAHLEMEDFDRDRDRVINRALEAGVDYIITVGTRLPDIE
                     KALRIAEKHPSLYVALGIHPHEAKGIGPGDYDNLRDLARGKKVVAFGEIGLDFYRNHS
                     PRDVQLSRFRELLRLGKDLRLPIIIHDRDAHEETIRILEEEKDGPWRGVFHCFSGDPE
                     MAQKVVKMGFYLSIPGTVTFKKSTVQHEVVKRIPLEKILLETDCPYLAPEPYRGKRNE
                     PAYILKTAEKIAELKSLCLEDVARITSLNVKLLFGVGEEIPKGKIVYPIRNSLYLNLT
                     NRCSNRCAFCWKNESFVVKGHDLELSREPDARELLEAIGDPKRYAEVVFCGFGEPLLR
                     LDLVKTVAAELKKKGAKIRIDTDGQANLVYGRNILPELKGLVDAVSVSLNAENAQKYH
                     RLCRSPFGEKGFEGVLEFLREAKKFIPEVTATALTMPGVDLEACRRLAEEELGVKFRS
                     RAYDEVG"
     gene            4199..4903
                     /locus_tag="VEL68_13235"
     CDS             4199..4903
                     /locus_tag="VEL68_13235"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HXZ36975.1"
                     /translation="MKKMLVISGVLFLVGSMMSNQTWSSDEKKDRFVVDRFTEGLDEK
                     GIPKGWDQEKERGPQSKITVGQEKEFHFVHLLSVNDGFGIKKDLSFDIRKYPYLTWRW
                     KATRLPKGGDIRKHETDDQAGQIYVLFPRFPAMINTRSMGYIWDTAAPAGTSGTSTAY
                     SKMKYVVLQSGPDKLGQWIWETRNVYEDYKKCFQEDPPTVGGVLLYINTQHTQSSAEI
                     DFGEIFFSTHPPEEFK"
     gene            5231..5392
                     /locus_tag="VEL68_13240"
     CDS             5231..5392
                     /locus_tag="VEL68_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXZ36976.1"
                     /translation="MKKYTCTICGYVYDPAVGDSENGIKPGTPFEKLPDSWTCPVCGA
                     SKSDFSPED"
     gene            5579..7663
                     /locus_tag="VEL68_13245"
     CDS             5579..7663
                     /locus_tag="VEL68_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXZ36977.1"
                     /translation="MTESGKKPGKSNHLIREKSPYLLQHAENPVAWYPWGEAAFQKAR
                     EEDRPIFLSIGYATCHWCHVMAHESFEDEEVAAVLNRHFVSIKVDREERPDVDQVYMS
                     ACQALTGQGGWPLSIFMTPEGDPFFAGTYFPKTGRLGMMGFTELLQRVATLWRTDREK
                     ILTGSGEIRQALRKEARPGFADAPLELGVLKKGYAQFERTYDERWGGFGSAPKFPTPH
                     HLTFLLRWLRRSEDLQARRMVERTLEAMRQGGIFDQLGFGFHRYSVDEKWLIPHFEKM
                     LYDQALLAMAYTEAYLALGEEECGKVTQEIFTYILRDMTSREGGFYSAEDADSEGHEG
                     RFYVWKPGEVRSHLGKEEGDLFCRFYGVSPEGNFEEGTSVLHIPRPVEHFAAGEKMDP
                     KELGKILERGREKLFHVREKRIHPLKDDKVLTSWNGLMIAALAKGYQALRDPLFTDAA
                     CRAADFILDRMRTPQGHLYHRWREGEVAIFGFLEDYAFLVWGLIELYEATFRVRYLEE
                     AIHLNQRMIELFGDKDRGGFYFSGRDNESLITRSKELYDGATPSGNSVAALNLLRLAR
                     MTGKIDLEKEVEGLLRVFSSTVAEAPMAFTQFLNFLDFYLGPSQEIVLVGDPAGETTR
                     AMTAMIQRTFQPNKVFLFRAEDETGNELGILCPFVEGMKSIGRKATVYLCEGYSCKTP
                     LTDLVALRGALK"
     gene            7675..>8163
                     /locus_tag="VEL68_13250"
     CDS             7675..>8163
                     /locus_tag="VEL68_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36978.1"
                     /translation="MGFKRLMRNRDFLLVMALLLGLFWGQGAKWTERTVIPVLGLVMT
                     LATMGVPGSIFLSPGNMVRPAFWGVALNYGVLGCLILALSGFFITDQDLWAGFVIMTA
                     VPPAVAVIPFSVILNGNQSLALIGTIGCYLAALGITPLIAYFFLGGAFSNLVKIVTIM
                     MEL"
BASE COUNT         1872 a         2107 c         2437 g         1747 t
ORIGIN      
        1 caggaatggg gagccaaggc agttctgcaa atctgccacg gcgggcgctg cacctcggca
       61 aggtacatgg gatgccagcc catcgcccct tcggccatgc ccagctatac tggggaaatg
      121 gcgcgggaaa tgactctgga ggaaattgag gccaccatgg actccttcgc cgaagcagcc
      181 cggagggcca agaccgcggg tttcgacggg gtggagcttc acgggaccca tggctatcta
      241 ttggctcagt ttctttctcc ttataccaac cgccgggagg atgtctacgg gaaggaccgg
      301 ggactctttg ccttgcagac cctggaaagg gttcgggcca gagtcggatc cgactacctg
      361 gtgggctacc ggatgagcgg ggaggagttc atcgatgggg gcgtcaccct gcaggaaacc
      421 aaggcctttg cccgcaggct ggaggcgaga ggcatcgatt acctccacgt gtccggtgga
      481 atcatcgagg tgggggagca tttcgtcatc cccatgtact tccccaaggg gcatctcctt
      541 cacctggccg aacaaatcaa gcaggcggtg aagataccgg tcgttgctgt gggagcgatc
      601 catgatccat ctttggccga agagtccttg aagaggaaac gggccgacct tatcgccatg
      661 ggacgggggc tgatcgccga cccggaattg ccgcggaagg tccagcagaa ccggcctgag
      721 gatatccgta cgtgcctgcg ttgcaacgag gggtgcattt cccgggtccg gcagggcaaa
      781 actcagcgtt gcgcggttaa cgcggaagtg gggagggagc ggacgctgag gattcatccg
      841 gcttccaggc ccaagcaggt gtgtgtgatc gggggcggcc cggcgggaat ggaagcggct
      901 cgggttttgg ccgagagaag gcacaaagtc acgctgatcg agaaggaaaa cgaactcggg
      961 ggcctcctcc gttttgcttc ggtcccggat ttcaaggaag agctgcgggg atttctccag
     1021 tacctgaaga ctcaggtgaa aaagacggga gtggaagttc tgatggggcg caaggccagc
     1081 ctcgacctgg tgcgcgactt tcatccggat tcagtggtcc tggctgctgg ttcgaccatg
     1141 cccatcccgg ggattcctgg agtgggaagg ccttttgtag ccggcgccct ggacctgctt
     1201 tcgggaaaat tccagccggg agaccgcgtg gtggtggttg gaggggcggc catgggttgc
     1261 gaggtcgccg cccacttggc gtctttggga aggaaggtca cggtcgtgga aatgataaac
     1321 ggattggccc ttgacctgga gaatcgatgc cgcgtggccc tcctccagtt gctgaaggaa
     1381 cggagcgtcg atatccttac caactggaaa atggaggcga tcggagaccg gatggttctt
     1441 ctgtccgatg gaaaccggaa caagaaagaa gtgaccgccg attccgtgat cctgaccctt
     1501 ggactcaccc ccaaccagga gttgctggag cccctgaccc gcaatttccc cgaagtccag
     1561 gtcatcggcg actgtctgga gccgcgcaag gtctaccagg ccattcacga aggagccttt
     1621 gccgggagag caatttaggg gtaacagttt tacgcagatt tccaggatga gaccaccctt
     1681 tattatccaa agctttgcca atttataacc tgcgtgaatc tgcgaaaatc tgcggatcga
     1741 ataaaaaaag gagagagatg tatgagtgaa attcggaatg tggccatcat aggggcaggg
     1801 actatgggac attcattggc ccaggttttt gcccagggag gatacaacgt ctggctgaat
     1861 gacgttaagg acgaaatcgt ggccagggcc aagaagttga tcgcctcgaa tctggatacc
     1921 cttattgagc tggggaatgt ggagaagagc cagaaagaac ccattcttcg gcggattcat
     1981 accaccgtga agctggaaga agcggggcag aacgctgatt ttgtcatcga ggcaatcata
     2041 gaggatcagg ccgccaaaaa agagattttc agcaggctgg acaagacctg cccgtccgga
     2101 gccatcctgg ccagcaacac ttcctacatg gacatcttca agttcgtaga aacgaaacga
     2161 ccggacaagg tcctcatcac ccattggttt gccccgcccc acatcgttcc gctggtggaa
     2221 atcgtaagag ggccgcggac ctctcaggag gtggtggatc gggtaaaggc ccttatgatc
     2281 caggtgggga agaagcccat cgtgatcacc aaattcctgc ccggattcat cgccaaccgc
     2341 ctgcagagcg ccctgggcaa tgaagttttc aacctgctag accaggggta cgcttcgccg
     2401 gaggatatcg atacagccac caaggccagt ttcgccttgc gcatgccaat cttgggcctt
     2461 gtcaagcgga tggatttcgc cggcttggat ttgacccaaa agattctgag caatcagacc
     2521 tacaaggtcc ccccccagaa caccaaatcc aagaccgtgg agcggcttgt gtcccaagga
     2581 aagctggggg tgaagaccgg cagcgggtac tacgaatacg gcggtcgttc caccgaagag
     2641 atcatgaagg aacgggacat gaagctgatc aagttgagag agttcttgaa ggagatgggc
     2701 gagttataaa atcgttgctg gtggcttgtt actggtcgct ggttggagac tcggccaggg
     2761 attttaaacc agcgagaagg gccgaactct gaactcctaa cgcattttag acctcaatca
     2821 tgtttgtcga ttcgcacgcc catttggaaa tggaggattt cgaccgggac cgggaccggg
     2881 tgatcaaccg ggctctggag gccggggtgg actacatcat cactgtgggt accaggcttc
     2941 cggacatcga aaaggccctt cggatcgccg aaaaacaccc ttccctctat gtcgctttgg
     3001 gcatccatcc gcatgaagcg aaaggcatcg gcccagggga ttatgacaat ctgcgcgact
     3061 tagccagggg gaaaaaggtc gtggccttcg gggaaattgg gttggatttt taccgcaacc
     3121 attctccccg ggatgttcag ctgtcccgtt ttcgcgaact tctgcggctg ggaaaggacc
     3181 tccgactgcc catcatcatt cacgaccggg acgcccatga ggaaaccatt cgtatcctgg
     3241 aggaggagaa ggatggaccc tggcgagggg tttttcactg cttctccggg gacccggaga
     3301 tggcccaaaa ggtggtcaaa atgggcttct acctatccat ccccgggacc gtgactttca
     3361 aaaagtccac tgttcagcat gaagtggtga agcgcattcc tctggaaaag atcctgctcg
     3421 agaccgattg tccctatctg gcccccgaac cttaccgggg caaaaggaac gaaccggctt
     3481 atatcctcaa gacggcggag aagatcgcgg agctcaagag cctgtgcttg gaggacgtgg
     3541 cccgtatcac ttccctgaat gtcaagctcc tattcggggt gggggaggag atccccaagg
     3601 ggaagatcgt ttaccccatc cgtaattctc tttacctgaa tctcaccaac cgctgttcca
     3661 accgctgcgc cttctgctgg aagaacgaat cttttgtcgt gaaaggccac gatctagagc
     3721 tttcccggga accggatgcc cgggaattgc tggaagccat cggggacccg aaacggtacg
     3781 ccgaagttgt cttctgcggc ttcggggaac cgctgctgcg gctggacttg gtcaaaaccg
     3841 tggctgcgga gctgaagaaa aaaggagcaa agattcgcat cgacacggac ggccaggcca
     3901 acttggtcta cggccggaac atcctgcccg agctgaaggg gctggttgat gctgtgtcgg
     3961 tgagcctgaa tgccgaaaac gcccagaaat accatcgact ctgtcggtct cccttcgggg
     4021 aaaagggttt tgagggggtt ttggaatttc tccgcgaggc caagaaattc attcctgagg
     4081 ttaccgcaac cgcattgact atgccgggag tggacctaga ggcctgccgc aggctggccg
     4141 aagaagaact gggggtgaag ttcaggtccc gggcttatga tgaggtggga taagaattat
     4201 gaaaaagatg ttggtgatct caggggttct cttcctggtc gggtccatga tgagcaacca
     4261 gacctggtca tcggatgaaa aaaaggacag gtttgttgtc gaccgcttta cagaagggtt
     4321 ggacgagaag gggattccca agggctggga ccaagagaag gaacggggcc cccagtccaa
     4381 aatcaccgta gggcaggaaa aggaatttca tttcgtccat ctcctgagcg tcaatgacgg
     4441 ttttggaatc aagaaggacc tctctttcga tatccgcaaa tacccttatc tcacctggag
     4501 atggaaagcc acccggcttc ccaagggcgg ggacattcgg aagcatgaga ccgacgacca
     4561 ggcggggcag atttacgttc tcttcccccg ttttcccgcc atgatcaaca cccggtccat
     4621 gggatacata tgggacacag cggcccccgc ggggacttcc ggaacgagca cggcctacag
     4681 caagatgaaa tacgtggtcc tgcagagtgg accagacaaa ctgggacaat ggatatggga
     4741 aacccgaaat gtctacgaag actataagaa atgcttccaa gaagaccccc ctaccgtggg
     4801 aggagttctg ctctacatta atacccagca tacccagagt tcggcggaaa tcgactttgg
     4861 ggagattttc ttctccaccc atccgcccga ggaatttaag taatcggttt tcctgtatta
     4921 tcaggcgtcc ttcgggcctc cttttatcag attatcggaa ttagggtctg gataaaaaaa
     4981 ccatggcaaa cggggaaata ttcgatacaa tatttttagg gcatcgaagc gccatcggcc
     5041 tagttgatga accagtttgg atggctagaa cttcccaacc aatttctata ccctttcctg
     5101 gctatatccg tcgagaaagg agttgaagaa agaacctcct gacttcaacg tgaatgaaaa
     5161 ttttgctctg acccgatttt tcacgccttt tcggaaggcc tgacaaaaaa aacaaaaagg
     5221 agagggagac atgaaaaagt atacctgtac catttgcggg tatgtgtatg accccgctgt
     5281 gggggactcg gaaaacggaa tcaaaccggg gacccccttt gagaaactac ccgatagttg
     5341 gacctgcccg gtctgtggag cctccaagtc ggatttttct cccgaagatt gattcccggg
     5401 gattggccgg tcccattttc tccccgctgg gagggatttt cttccgaact ctttccgatc
     5461 cccccacctt ccctggttaa aaagggaggc ctggggggat ttttcatctc ccgggacggg
     5521 caaccagaat cgagcaccca gtaacccgtt ttcccatgaa ctccatcagg ggcagcgtat
     5581 gaccgagtca ggcaagaagc cggggaaatc caatcatctc atccgggaga agagtcctta
     5641 cctcctccag cacgcggaaa atcccgtggc ctggtatccc tggggcgaag cggcttttca
     5701 aaaagcaaga gaagaggata ggcccatctt tctatccatc ggctacgcca cctgccactg
     5761 gtgccatgtg atggcccacg agtctttcga agacgaggaa gtggctgcag ttctcaaccg
     5821 gcatttcgtc tccataaaag tggaccggga ggaacgcccg gatgtggacc aggtctacat
     5881 gtccgcatgc caagccttga cgggccaggg aggctggccc ttgtccatct tcatgactcc
     5941 tgagggagac cccttcttcg ccgggactta ttttcccaaa accggccggc tgggcatgat
     6001 ggggttcacc gagctcttac agagagtcgc caccctctgg cgcacggacc gggagaagat
     6061 cctcaccggc agcggagaaa tccggcaggc gctgcgaaag gaggcgcgcc ccggctttgc
     6121 ggatgcaccc ctggagctgg gtgtgctgaa aaaaggctat gcccagtttg aaagaactta
     6181 tgatgagcga tggggaggtt tcggaagcgc tcccaaattt cccacccctc atcatctcac
     6241 cttcctgctt agatggcttc gccgaagcga agacctacag gcccgaagga tggtggaaag
     6301 aactctggag gccatgcgac agggggggat tttcgatcaa ctgggctttg gatttcaccg
     6361 ctattcggtg gatgaaaaat ggctcattcc ccacttcgaa aaaatgcttt acgatcaggc
     6421 attgctggcc atggcttaca cggaggccta tctggccctg ggggaagagg agtgcggaaa
     6481 agtcacccag gaaatcttca cctatatcct gcgggacatg acctcccgcg agggagggtt
     6541 ttattcggct gaggacgcgg acagcgaggg gcatgaggga cgcttttatg tctggaagcc
     6601 cggagaggtc cgaagccacc tgggcaagga agagggggac cttttttgcc ggttttacgg
     6661 ggtgagcccg gaaggaaact tcgaggaagg gaccagtgtt ctccacatcc cccgcccagt
     6721 tgaacatttt gcggcggggg agaagatgga cccgaaggag cttgggaaaa tcctggagcg
     6781 aggccgggaa aagctctttc acgtgcggga gaaaaggatt catccgctga aggacgacaa
     6841 ggtcctcacc tcctggaacg ggctgatgat cgcggccctg gccaagggct atcaagcctt
     6901 gcgggatccc ctcttcaccg atgccgcttg ccgggccgcg gattttatcc tggaccggat
     6961 gcggacaccc caggggcacc tttaccaccg atggcgggag ggggaagtgg ccattttcgg
     7021 tttcctggag gattatgcct tcctggtctg gggattgatc gagttatatg aggccacctt
     7081 tcgggtccga tacttggagg aagcgattca tctcaaccag aggatgatag aactcttcgg
     7141 ggacaaagac cgcgggggtt tttatttctc aggaagggat aacgaatcgc tcatcactcg
     7201 ctcgaaggaa ctgtatgatg gggctactcc ttcagggaac tcggtggctg ctctgaacct
     7261 tctgcgtctg gcacggatga ccggaaaaat agacttggag aaagaggtgg aaggtttgct
     7321 tcgggttttt tcttctacag ttgccgaggc ccctatggct tttacccaat ttttgaactt
     7381 tctggatttt tacctgggtc cgagtcagga gatcgtccta gtaggggacc cggccgggga
     7441 gacgacccgg gccatgaccg ccatgattca gcggacgttc caacccaata aagtcttcct
     7501 gttccgggcg gaggacgaga ccgggaacga actcggcatt ctttgtcctt tcgtggaggg
     7561 gatgaaaagc attggccgga aggccaccgt ttatctttgc gaagggtatt cctgcaaaac
     7621 ccctttgacc gacttagtcg ccctccgggg cgcattgaaa tagagagggg gacgatggga
     7681 ttcaagagat tgatgcggaa tcgagatttt cttttggtta tggccctctt gctcgggtta
     7741 ttctggggac agggtgccaa gtggaccgaa agaacggtga ttccggtttt gggcctggtt
     7801 atgaccctgg ccacgatggg agttccgggg agtatttttc tttctcctgg gaacatggta
     7861 cgcccggcat tttggggcgt cgctttgaac tacggcgtgc tgggatgttt gattctcgcc
     7921 ttgagcgggt ttttcattac cgaccaggac ctgtgggctg gttttgtgat catgaccgcc
     7981 gtgcctccgg cagtggcggt catccccttc agcgtgatcc tgaacgggaa ccagtccttg
     8041 gccctcattg gaaccatcgg atgctacctg gccgccctcg ggatcactcc tctcattgcc
     8101 tacttttttc tcggaggggc cttcagcaac ctggtgaaga tcgtgaccat catgatggag
     8161 ctg
//
LOCUS       DASPRR010000352        14000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_399420, whole genome shotgun sequence.
ACCESSION   DASPRR010000352 DASPRR010000000
VERSION     DASPRR010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14000)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14000
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399420"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VEL68_13255"
     CDS             complement(<1..347)
                     /locus_tag="VEL68_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36979.1"
                     /translation="MNSKPVNDRTWARGGIELSRHFAGSTLKLREKAFSREDYDPARL
                     FKWGQMMALAVVRMMEGTEKAFGAKGQEIMNQVLVDLGREIGREVLNNYAAVRGTKEI
                     EAISKFVTYVNEEV"
     gene            614..1237
                     /locus_tag="VEL68_13260"
     CDS             614..1237
                     /locus_tag="VEL68_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36980.1"
                     /translation="MLNPLTIFAILAAAFSLVAFVAAVWSLRKRKFLGGTIGLLLGLL
                     FLSLGGLFGTISIATQGYRALVREEVAAVVEIQPVGTQEFNAHFRLADGEEISFLIAG
                     DEIYVDAHILKWKPLANFLGLHTAYELDRVAGRYTQLEDERSKPRTVFSLSQDKAVNM
                     FHLRQKYAFLTPLLDAQYGSGTFVPADKPVELEVRVSTSGLLIRKGR"
     gene            complement(1249..1431)
                     /locus_tag="VEL68_13265"
     CDS             complement(1249..1431)
                     /locus_tag="VEL68_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36981.1"
                     /translation="MKIGGVSIILILGIINFLLLLFQVSSGMRWIKVKMTLHRKTGIT
                     LLITAFFHGLLAILTG"
     gene            complement(1593..1973)
                     /locus_tag="VEL68_13270"
     CDS             complement(1593..1973)
                     /locus_tag="VEL68_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HXZ36982.1"
                     /translation="MNRRDFVVASGVLVAGVLVPRGTAWANKAETKIELPQSAARGSE
                     ITIRVTVTHSANNFFHHVEWLWIQMNGKEIDRWEYSATNLPESATFTKEIKYVVDGDL
                     EFKAKASCNLHGSANEAVAKVTAS"
     gene            2184..3743
                     /locus_tag="VEL68_13275"
     CDS             2184..3743
                     /locus_tag="VEL68_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HXZ36983.1"
                     /translation="MKKNKQQTKVRMGIDFGTTRTVVARVEGGNYPLVTFEAEWGDGQ
                     EWYPSLIAGKGSKRVFGWEAWSKQWEENWQVIRSLKRYLSHANPATCLQVGEEKVPLL
                     TLLIEYLSALHRDLLENSNLQLRDSAILEVMVGVPANANNNQRFLTLEGFRRSGFEVL
                     GLINEPSAAGVEYAFRYRKQASERKREFLLVYDLGGGTFDLSVIQMSDLSHRVMTTAG
                     MEHLGGDDFDAILASLGAERARLKPEIRSNFWLLEECREKKESLHFNTKKILLDLTRA
                     QGQELEVTVTTAEFYERCRPLVEQTLAAVEATLGRPALNEASPGWAENAALYLVGGAA
                     ELPLISQMMREGYGRRIRKSPYPRAATAIGLAIAADEMAGFTVQDRFTRHFGVWREEE
                     EGRQISFDPIFPKDTLLPTKGEPPLIKSRRYHPAHNIGHFRYLECSHLNEQGQPTGDL
                     TTWDEIRFPFDPSLTGGNGWEMASVQRFEPSMELQAEEIYTCDSQGIVKVTLINHSNG
                     FQKSYQLRHGA"
     gene            3804..4997
                     /locus_tag="VEL68_13280"
     CDS             3804..4997
                     /locus_tag="VEL68_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HXZ36984.1"
                     /translation="MNKSYDVIIIGAGPGGCSTAISLARRGCDVLLLEKERFPRGKVC
                     GDGISPPALETLDRLGVLPEIMRKDPWKVAGVDLFSPAGQVVTVSFSHLKGPFPFGLV
                     MPRKEFDFLVFQQVRKYSNLEALENWEFKDFIDQNGSIKGVKAQSEGQKREFLAKFLV
                     GADGAYSVVARKAVSRKQNFQQGAFGIRAYFRGVKDLAHRIEIHCVRSILPGYGWVFP
                     TGEDSANVGVGIASCHLKEKNIRNLFQTFTEGNPLVKERLRQARMVENSLQGGPIPLG
                     SFFRRRSRKNVLLVGDAGGFTDVLTGEGVYFALRSGECAAEAIHSALSLPGELEKAGR
                     AYENLWRNALPSREFFFGSLVQRLIVREFFLNRNIARAVKKPSMARDLASILCHEKSK
                     IGLFF"
     gene            5368..6804
                     /locus_tag="VEL68_13285"
     CDS             5368..6804
                     /locus_tag="VEL68_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36985.1"
                     /translation="MKTISKMSWMLALVTFVFTFSFGPWGVVKAMAFATPKAVFDVNK
                     MGDMSDFDPNNPVIPTGDTIKIACVASHSGPAAIVGQMYWMNLLWVAHDINKRGGILV
                     DGKKKLVQIIKADHMSKPDQGKKVCERMVLQDKVHVLWGTDGSNLMKVINDTASKYKV
                     IAQNTAALSDELNDATNFSRYSFFTSFSCDQIGRGIAYYYAQIRKKEKKFYILCQDYM
                     FGHSMAEGFKKGLKQYYPEGQIVGEDYHKLFLTDFAPYLEKIKGSGAEVVYTGDWVPD
                     AANLLKQARQMGITLPFAHIFLDEPNFLHEVGVEGTKGLVQLSQYGTEPPTFKTPEQI
                     KYYKTWNNLWKTKWQAPFNTRLYEHGTGNFGSYIMQTYWMLSVMERAKSTDPEKMIKV
                     WEGDTYQYVNGKIMQMRPCDHKAIQDLHIFEFVPPDQQKESFNIPPYYWFQGTCAAGP
                     MFKIPADKVLPLMDGNLDRCKGKNDWGQ"
     gene            6923..7795
                     /locus_tag="VEL68_13290"
     CDS             6923..7795
                     /locus_tag="VEL68_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36986.1"
                     /translation="MESTTAMYVAQGIHGLAYGMVLFLVASGLTLIFGMMDILNLAHA
                     AFFMLAAYFCYQFLSLTGNFWAALLLAPIVTAFFGILLERFLLRKIHAFGLMSQLTLT
                     VGVSLVILAGVKIFWGTESLPVKIPPILSGLVKMGGMDYPVYRLFIIVLSLVVLAIMA
                     LLLYKTRIGKIVRAAVSDADMVNALGINIPLVFTFVFGVGIWLAGIAGVVIAPILTVF
                     PGLADQMGMDAFVVVITGGFGSLTGAFIVSLIFGLLSSYGVQFVSSLAPVLMFAFMAI
                     ILAIRPMGLFGERE"
     gene            7792..8790
                     /locus_tag="VEL68_13295"
     CDS             7792..8790
                     /locus_tag="VEL68_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ36987.1"
                     /translation="MMKKKLRWILWVGLIVILALYPRVFGIYYTNFFVTFAVFAVYSV
                     SFNLLLGYTALFSFGHAMFFGVGGYGTALALKHIQGFPLLPAILIGAGSAVVLALILC
                     PIVVRVTGSAFSMLHLAFGQLMYVLALKLRNITGGEDGIGGFPIPPFSIPGIVSIDMK
                     APENFYYFAVVVLGVSLWIMWFFTKTPFGQIQVGVRDNAMRVDYLGFKVPQTKAVVYV
                     VAGAFAGVAGSIFALFQNLIAADSGFGILVSFGAVINTLVGGLGSFFGPIWGTAIMQI
                     LEEVTTRYTDRVELVAGLILILVIMFAPMGFVGGVRILKQKWLAHSAPKVKLEKVS"
     gene            8787..9533
                     /locus_tag="VEL68_13300"
     CDS             8787..9533
                     /locus_tag="VEL68_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36988.1"
                     /translation="MNILESRGLYHDFKGLEVLFDVNLQVEEGERHAVIGPNGAGKTT
                     LFNVITGTYHPSRGQVFFKGKEVTGSKPYQMARLGMGRSFQITSTFNRLTAFQNIRLA
                     VLSKKGVRFQLFRKVDKMEDITKETDEILKRINLDRERDMPAGMLSYGKHRSLEISMA
                     LGTDPDLVMLDEPTAGMSRDETHYAVELIRRLTEGKTAVIIEHDMDVVFSLADRITVL
                     HYGQILATGPPAEIRQNQAVKDAYLGELEA"
     gene            9667..10383
                     /locus_tag="VEL68_13305"
     CDS             9667..10383
                     /locus_tag="VEL68_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ36989.1"
                     /translation="MLLDVKELNTYYGMSHVLQGISLNVNQGELVTLLGRNGMGKSTT
                     LKSIMGLVKPKSGSVVFEGKTITGYPPYKAARSGIGYVPEDRRIFPNLSVLDNLLLGI
                     KGGKIVDPNSPNTWTVERIFRHFPMLEKRAQQQGRLLSGGEQQMLTIGRSLMGNPSLL
                     LVDEPTEGLAPLLVKEVRDILEEINKAGVSILLVEHNIKVALSLADRVYLMGKAHIGF
                     AGTVAELEANPETKAKYLEV"
     gene            11019..12557
                     /locus_tag="VEL68_13310"
     CDS             11019..12557
                     /locus_tag="VEL68_13310"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ36990.1"
                     /translation="MNFSAHIERSCKMNPHKTCVVQGDLRLTYAQMAERGNRLARALV
                     ERGLKPGDRVAIFLTNCFQFAEMLYAIAKIGGIFLNLNFRLREEETSYILRNAEPKIL
                     ILGDRYVELINNIRPRLSFIECFIVMGQHPSDMQDYESVLSHQSPAPYPCISVQGDDT
                     ACLIYTSGTTGLPKGAMLTHANLVTPLKDKYFIPAGTLLLNVPMYHIAGVLSTLVPLY
                     RGDTQIVLPQFETGIFLEIVEKEKVATTYLVPTMLQGILDHPDFPKRDLSSLKAIGYG
                     ASPMPVKLLLRAKDLLKVDYTNYFGLTESTGIVSVLSPEDHRLEGGKDELGKKTRRLA
                     GIGHSIPEGEVRIVDDQDRDVPIGKVGEILARGKKIMKGYWNNPKATEETLRGGWLHT
                     GDLARMDEEGYLYLAGRKKDMIIRGGENIYPVEIENVLHSHPKVLEAAVIGVPDEYWG
                     EIVKAVIILRPGERSTSEEIIGYCRERLASYKKPAIVEFVDALPKNAMQKVLKNVLRG
                     EAPK"
     gene            12840..>14000
                     /locus_tag="VEL68_13315"
     CDS             12840..>14000
                     /locus_tag="VEL68_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ36991.1"
                     /translation="MDSISKIRSSLIFMGFVFAFSVFLFGMPQAMAFEPLKGDLSKYD
                     PNKLDLPTGDVVKIAMWDQVSGVNAYLAEAYSALLGFVAQDINSQGGIKVDGKMKKVS
                     FVLADTQGRPDPGKRAAEKAILQDKVNVFAGVAGSHVAKVGQGTAKEYKTIFMNVSAY
                     SDELMDLPDWNEYVFRTCGTSATVGKSLAIFYEKRPETKFYILAQDYLWGHSSAGAFK
                     KALKESKPKAQIVGEEYFPVYTKDFAPYLEKVRASGAEVIVQMAWGADNENLIKQSRQ
                     LGLKSPLNPEYYIPMASPFLDDTRPLEVIGGPAGRGLVLCTDFQLDRRVPNVKKLTDI
                     WTGLWKTWKAPYNTRLYQWPNGGWYRNLLSYYWYLQVVQKAGSTDTKKVIAAW"
BASE COUNT         3304 a         3526 c         3805 g         3365 t
ORIGIN      
        1 acctcttcgt ttacgtaggt gacgaatttc gagatggcct caatttcttt cgttcccctg
       61 acggctgcat agttatttaa aacttctcgc ccaatctccc gccctaggtc cacaaggacc
      121 tggttcataa tctcctgccc cttggcaccg aaggccttct cggttccttc catcatccga
      181 accaccgcga gcgccatcat ttggccccat ttaaagaggc gggccgggtc gtaatcttca
      241 cggctgaaag ccttttcgcg caatttaagg gtcgaaccgg caaaatggcg ggacaattca
      301 attccccccc gcgcccaggt tcgatcattg accggcttgc tattcataga gccctccctt
      361 taacatattt ttaaggagac ctttctttcg ataccagcaa gcgctttata tttaagcaga
      421 ccccggcggc gtttcattat gtcagagaag ggaaatttta tcaaatccgc aatttatcga
      481 atcctcctaa atctatcccg gaattgaatt tgaagaagag aattggaaag tcaggagaag
      541 tgagttatac ttcgctagtt tatggaaagg aaggggagtt caaaagaatg gtggtgcata
      601 gggggtgttc cgcatgctga atccgcttac gattttcgcg atcttagcgg ccgcttttag
      661 cctggtggct tttgtcgctg cggtctggtc cttgcggaaa agaaaatttt taggaggtac
      721 gatcggcctc ttgctgggtc tgttgtttct ttccttgggt gggcttttcg ggaccattag
      781 catcgccacc caggggtatc gggcactggt tcgggaggag gtcgccgccg tggtggagat
      841 acaacccgtg ggaactcagg aattcaacgc ccactttcgg ctggcggacg gagaggaaat
      901 cagttttctg atcgccgggg atgaaatcta cgtggatgcc cacatcctca aatggaagcc
      961 cctcgccaat tttctgggac tacatacggc gtacgaattg gaccgggtgg ccggccggta
     1021 cacccagttg gaagatgaac gttctaagcc ccgcaccgtg ttttccctct cgcaggataa
     1081 agccgttaat atgtttcact tacgccagaa gtatgcgttc ctgactccct tgctggatgc
     1141 ccagtacggt tcgggaacct ttgttcccgc ggacaaaccc gtcgaactcg aggtccgggt
     1201 gtcaaccagc gggctcttga ttcgtaaagg gaggtaagat ggccttttct agccagtcaa
     1261 aatggccaga aggccatgaa aaaaagcggt gatcagaagg gttatcccgg tttttcggtg
     1321 taaggtcatt ttcactttga tccagcgcat tccggagctg acctggaaga gaagcaaaag
     1381 aaaattaatg atccccagaa tcaggatgat cgaaaccccg ccgattttca taatacctcc
     1441 gctcaaaaag gcttcaggta caaggggcta ggtcctggct cacgggccag acatttcctg
     1501 tctggccgag ggccctttac ctctacccct tctctcgttc ccgctcctag cagcttggtt
     1561 gcccacgtgg aactcgcaac ccgcagccat ttttatgatg ccgttacttt cgcgaccgct
     1621 tcattcgcgc ttccgtgcag gttgcagctg gccttggcct tgaattccaa atcgccgtcc
     1681 accacgtatt ttatctcctt ggtaaaagtg gcactctcgg gcaggttggt tgccgaatat
     1741 tcccatcggt caatttcctt cccgttcatc tgaatccaaa gccattcaac atgatggaaa
     1801 aagttattgg cgctgtgagt cacggtcacc cggatagtaa tttccgaccc ccgagcggcg
     1861 ctttgcggca gttcaatttt agtttctgct ttgttcgccc aggcagtacc gcggggtacc
     1921 agaacgcccg cgactaagac gcccgatgcc acgacgaaat ctcttcggtt catatttcct
     1981 cccttcgagg ctggctatgg aaattagagc ctcaatgaaa attttagcag aggggaaaat
     2041 caattcaaag cgtaaatatt tggtactttc ctgcgccttg cacccgggtt ccctaagagc
     2101 ccccgccccg tgatttcaaa gggcaagcac caaggaaccg ttgcctttct tggcattcct
     2161 ggtaaaatat tgaatcaggg aaaatgaaga agaataaaca acagactaaa gttcgaatgg
     2221 gaatcgactt cggcacaacc cgcaccgtgg tggcccgggt ggaagggggc aattaccctc
     2281 tggtcacttt tgaggctgag tggggggacg ggcaggaatg gtatccttct ctcattgccg
     2341 ggaagggatc gaagcgggtc tttgggtggg aagcttggtc caaacagtgg gaagaaaact
     2401 ggcaggtcat ccgctccctg aaacgttacc tgtcccatgc caacccggcc acttgccttc
     2461 aagtggggga ggaaaaggtt cccctcctga ctctcctgat cgaatacctg agcgccctcc
     2521 acagggacct tctggaaaat tccaaccttc agctccggga ttcggcaatc ctcgaggtga
     2581 tggtgggcgt cccggccaat gccaacaaca atcagaggtt cctgactctc gagggatttc
     2641 gccgttcggg ttttgaagtc ctggggctga tcaacgaacc atcggccgcg ggggtggagt
     2701 atgctttccg ataccggaag caagcctcgg agcgaaagag agagttcctt ctggtatacg
     2761 atctgggcgg ggggaccttc gacctctcgg tcattcaaat gagtgatctc agccaccggg
     2821 tcatgaccac cgcgggaatg gagcacctgg gaggagatga ctttgacgcc attttagcca
     2881 gcctgggcgc tgaacgggcc aggctgaaac ccgaaattcg atcgaatttc tggcttctgg
     2941 aagagtgccg cgagaaaaaa gagagccttc acttcaatac caagaaaatc ttgctcgacc
     3001 tgacacgcgc gcagggtcaa gagctagaag taaccgtcac cactgcggag ttttatgaac
     3061 ggtgccgacc gctggtcgag cagaccctgg ccgccgtcga ggccacgctg ggccgaccgg
     3121 ctttaaacga agcctccccc ggatgggcgg aaaatgcagc tctttatctc gtcggaggag
     3181 cggccgaact tcccctgatc tcccagatga tgcgggaggg gtacggccgc cggattcgca
     3241 agtcccctta cccgcgagcc gccacagcta tcgggctggc gattgccgcc gacgagatgg
     3301 ccgggttcac ggtccaggat cgctttaccc gccatttcgg agtgtggagg gaggaagaag
     3361 aaggccggca gatctccttt gaccccattt tccccaagga cacccttctt cccacgaaag
     3421 gagagccgcc cctgatcaaa tcgcgtcgtt accatccggc ccacaacatc ggccacttcc
     3481 gttacctgga gtgcagccac ctcaatgaac agggccagcc cacaggggac ctcacgacct
     3541 gggacgagat tcgttttccc ttcgatccga gcctcactgg gggcaatgga tgggagatgg
     3601 cttccgtcca gcgcttcgag ccctccatgg agcttcaggc ggaagaaatc tatacttgcg
     3661 acagccaggg gatcgtgaag gttaccctga tcaaccattc caacggtttc caaaaatctt
     3721 accagctccg ccacggggct tagttaatac ctgatacgcc ttcgtagggg cgacccggcg
     3781 ggtcgcccct accttacgac cccatgaaca aaagctatga tgtaatcatc ataggcgccg
     3841 ggcccggtgg gtgcagcacc gcaatcagtc tggcccgccg ggggtgcgac gttctgctat
     3901 tggaaaagga acgattccca aggggcaaag tgtgcgggga tggaatcagc cctccggcgc
     3961 tggagaccct ggaccggctg ggggtcctgc cggaaataat gagaaaagat ccatggaagg
     4021 tcgccggcgt ggatctcttt tctcccgccg ggcaggtggt cactgtctct ttttcccact
     4081 tgaagggtcc ctttcccttc ggcctggtca tgcccaggaa agaatttgat ttcctagttt
     4141 tccagcaggt ccggaaatat tcgaacctag aagccctgga aaattgggag tttaaagatt
     4201 tcatcgatca gaacggctcc attaagggag tcaaggcgca atccgaaggc caaaaaaggg
     4261 aatttttggc caaattcctg gtcggggctg acggcgccta ttcggtggtc gcgcgaaaag
     4321 cagtttctcg aaaacagaac tttcagcaag gagccttcgg aataagggcc tatttccggg
     4381 gggtaaaaga tttagcccat cgcatcgaga tccattgcgt ccgctccatt ctccccgggt
     4441 atggctgggt tttccccacc ggggaagact cggccaatgt gggagtcgga atcgcgtcat
     4501 gccacttaaa agaaaaaaac atccgcaatc tttttcagac ctttacggag gggaacccgt
     4561 tggtaaaaga aagattgagg caagcccgga tggtggaaaa ttccctccag ggaggcccca
     4621 tccccctggg ttcttttttc cggcggcgaa gccggaaaaa cgttctgtta gtaggcgatg
     4681 ccggcggctt tacggatgtc ctgaccgggg aaggggtcta cttcgccctg cgaagcggag
     4741 agtgcgcggc ggaggccatt cactcggcgt tgagtttacc cggagaattg gagaaagcag
     4801 ggcgggccta tgaaaaccta tggaggaacg cactcccgag tcgggaattt tttttcgggt
     4861 ctcttgtcca aaggctgatc gtgagggaat ttttcctgaa tcggaatatc gctcgagcgg
     4921 tcaagaagcc ctccatggcc cgggacctgg cctcgatcct ctgccacgaa aaatcaaaga
     4981 tcggattgtt cttttaaatt caattttttc ttgacaaggt tcgattttta ggttactcct
     5041 tgccccaagc cgggtctaac agcctgttcg gttgccacct tgttccatag ccttctgggt
     5101 cgaaggatga aaagaggatt ccgaaggttt cgctttgcgc ccaccaaaat gccttcattg
     5161 gctattaggg aattttgcca ttttcccgac cggaccattc catttttatg cttgaagata
     5221 gggaaccgtt tcttccgaag gttcaagttt tcaatttaga aagctgcggc acctgaagct
     5281 tgcctggaag gatgaaatcc gaggacgatt gaagaggtag aggagtccct tttgtaatct
     5341 actgagataa ggaaaggagg caggaagatg aagacgattt ctaagatgtc ctggatgctc
     5401 gcgctggtaa cctttgtttt caccttctct ttcgggccat ggggagtagt caaggctatg
     5461 gcttttgcaa cacccaaggc cgtctttgat gtcaacaaga tgggggatat gtccgacttt
     5521 gaccccaaca acccagtgat cccgaccggg gacaccatca agatcgcctg tgtcgcctcc
     5581 cactccggcc cggctgcgat tgtcggccag atgtactgga tgaaccttct atgggtggcc
     5641 catgatatta acaagagggg cggcatcctg gtggacggaa aaaagaaatt ggtccaaatc
     5701 atcaaggcgg accatatgtc caagccggac cagggaaaga aggtctgtga aaggatggtc
     5761 ctgcaggaca aggttcatgt cctctggggt acggacggca gcaacctgat gaaagtcatt
     5821 aacgacacgg ccagtaaata caaagtcatt gcccagaata ctgctgccct atccgatgag
     5881 ctcaatgatg ccacgaattt ctctcgctat tctttcttta cctctttttc ctgcgaccag
     5941 atcggacgtg gaatcgccta ctattatgcc cagatcagga aaaaggagaa aaaattttac
     6001 atcctctgcc aggactacat gttcggtcac tccatggcgg aggggtttaa aaaagggctg
     6061 aaacaatact atcccgaagg ccaaattgtc ggcgaggatt accacaagct tttcttaacc
     6121 gactttgctc cctacctgga aaagatcaag ggttccggag ccgaggtcgt ctatacaggt
     6181 gactgggtcc cggatgctgc caatctcctt aaacaggcaa gacagatggg aatcacgctt
     6241 ccctttgccc acattttcct ggatgagccc aatttccttc atgaagtcgg agtagaaggg
     6301 acgaaggggt tagtccagct cagtcagtat ggaacggagc cgccgacctt caagacaccc
     6361 gaacaaatta aatactacaa aacctggaat aacctgtgga agaccaagtg gcaggcgcct
     6421 tttaacacca ggctctatga acacgggacc ggcaacttcg gttcctacat tatgcagacc
     6481 tactggatgt tgagtgttat ggaaagggcc aagagcacgg acccggaaaa gatgatcaaa
     6541 gtttgggagg gggacaccta ccagtatgtg aacgggaaaa ttatgcaaat gaggccctgc
     6601 gaccataaag cgatccagga ccttcacatc ttcgaattcg tccctcccga ccagcagaag
     6661 gagtctttca acattccgcc ctattactgg ttccagggca cttgcgctgc gggcccaatg
     6721 ttcaagatcc cggccgataa ggtgctccct ttaatggatg gaaacctgga tcgatgcaag
     6781 gggaagaatg attggggcca ataaataagc gaacgcaaag tgcagaggac atagagccca
     6841 gagcaaaatc ctggtttcca atctgaagtt tttgacgctt gcgctctggg ctttgcgttc
     6901 ttctccccaa gaggtagaat cgatggaatc aacgaccgca atgtatgtcg cccaggggat
     6961 acacgggctg gcctacggga tggtgctttt tctggtcgct tccggcctca ccctgatatt
     7021 cgggatgatg gacattctga acctcgccca tgccgccttc ttcatgctgg ccgcatattt
     7081 ttgttaccag ttcttgtccc tgaccggaaa tttctgggcg gctttgcttc tggcgccgat
     7141 cgtcaccgct ttttttggaa ttctcctgga aagatttctc ctcaggaaaa tacatgcttt
     7201 cgggctgatg agccaattga cgttaaccgt gggcgtttcg ctcgtcatcc tggccggggt
     7261 taagatcttc tgggggacgg agagcctgcc ggttaagatt cctccgatcc tcagcggatt
     7321 ggtgaagatg ggaggcatgg actatccggt ctatcggctt ttcatcatcg tgctgtcgct
     7381 ggtggttttg gcgatcatgg ctttgctcct gtataaaacg cgtatcggca aaattgtgcg
     7441 cgcggccgtt tcggacgccg atatggtcaa tgcccttggc atcaatatcc ccctggtctt
     7501 cacgtttgtc tttggagtcg gtatatggct ggccgggata gccggggtgg ttatagcacc
     7561 catactgacc gtatttcctg ggctggcaga ccagatgggc atggatgcct ttgtggtagt
     7621 gattaccggg ggattcggca gtctcacggg agcgtttatc gtctccctca tatttgggct
     7681 gctgagttcc tatggggtac agttcgtctc ttcccttgcg ccagtcctga tgttcgcctt
     7741 catggccata atcctggcga tccgacccat gggtctcttt ggagaaagag aatgatgaaa
     7801 aagaagcttc ggtggatctt atgggtcggg ttaattgtaa ttctggcatt atacccaaga
     7861 gtttttggta tctactatac caactttttt gtgacgttcg ccgtctttgc cgtctattcc
     7921 gtctctttta atttgctcct cggttacacg gcgcttttca gctttggcca tgccatgttt
     7981 ttcggtgtcg gcggttatgg cacggcactg gccttgaagc atatccaggg gttccctctt
     8041 ctgccggcga tcttgatcgg cgcgggatcg gcagttgtcc ttgccttgat cctatgcccc
     8101 atcgtcgtca gggtcaccgg ctcggccttt tccatgctgc acctagcgtt tggccagctc
     8161 atgtatgtcc tggccctgaa attaagaaat atcaccggcg gagaagacgg aatcggaggg
     8221 tttcctatac ccccgttcag cattccgggg atcgtctcaa ttgatatgaa agctccggag
     8281 aacttttatt actttgccgt cgtcgtcttg ggggtgagcc tctggattat gtggttcttt
     8341 acgaagacgc cttttggcca aatccaggtg ggtgtccggg ataatgccat gcgggtcgac
     8401 tatctgggtt ttaaggtgcc gcagaccaag gccgtggttt atgttgtcgc cggcgctttt
     8461 gccggggtcg cgggttccat ctttgcgtta tttcaaaacc tgattgctgc cgatagtggc
     8521 ttcggcattt tggtttcttt tggggccgtc attaatactc tcgtcggtgg attgggcagc
     8581 ttctttggcc cgatctgggg cacggccatc atgcaaattc tggaggaagt gacgacccgt
     8641 tatacggatc gggttgagct ggtggcgggc ctgattctta ttctggtcat catgtttgca
     8701 ccaatggggt ttgtgggcgg ggtcagaatt ctgaaacaaa aatggctggc ccattctgcc
     8761 ccaaaggtta aattggagaa ggtttcatga atatcctgga gagcagaggg ttgtatcacg
     8821 attttaaggg cttggaagta ttgttcgatg tcaatttgca ggtggaagag ggagagcggc
     8881 acgccgtgat tgggcccaat ggagcgggga aaacaacgct cttcaacgtc atcaccggaa
     8941 cgtaccatcc gagccggggg caagtgttct tcaaaggaaa agaggtaacc gggtccaagc
     9001 cataccaaat ggcgcgcctg ggcatggggc gctcttttca gatcaccagc acctttaacc
     9061 ggttgaccgc ttttcagaac attcgactgg ccgtcctatc caagaagggg gtccgcttcc
     9121 agctgtttcg caaagtggat aagatggagg acataaccaa agagacggac gagatattaa
     9181 aaaggatcaa tttagaccgg gagcgggata tgcccgccgg gatgctgtcc tacgggaaac
     9241 atcgttccct agagatcagc atggccttgg ggacggaccc ggacctagtg atgctggatg
     9301 agcccacggc cggcatgtcc cgcgatgaaa cccattatgc cgtcgagctg atcagacggc
     9361 tcacggaggg caaaaccgca gtcatcatcg agcacgacat ggatgtagtc ttctccctgg
     9421 cggatcgcat caccgttctg cattacgggc agatcctggc cacggggcct ccggcggaga
     9481 tccggcagaa tcaggcggtc aaagatgcct acctggggga actggaagcc taaatttaac
     9541 agagtttcga gtttcaggtt gagaactaac ggccctagag gggatgcatc tctatatagc
     9601 cgccctactt caaacctgaa attcgaaact cgaaaaccga aacatttaag aggtggggta
     9661 agtggaatgt tactcgatgt caaagaattg aatacctact acggaatgag ccatgttctg
     9721 caggggattt ccctaaacgt taatcagggg gagctcgtca ccctgctggg ccgcaatggc
     9781 atggggaaaa gcaccactct gaaaagcatc atggggctgg tcaaaccgaa atcagggtcg
     9841 gtcgtcttcg aagggaaaac gattacgggt tatcctccct ataaggcggc taggtcggga
     9901 atcggctacg tccccgagga ccgtcgcatc ttccccaatc tttcggtgct ggacaatctg
     9961 cttttaggga tcaaaggggg aaaaatcgtg gatccgaaca gtccgaacac atggaccgtg
    10021 gaaaggatct ttcgtcattt tcccatgctg gagaaaagag cccaacagca agggaggttg
    10081 ttgtccgggg gggaacagca gatgctgacc atcggccgtt ctttgatggg caatccctcg
    10141 cttctgctgg tggacgaacc gaccgaaggc ctggctccgc tcctggtgaa ggaggtccgg
    10201 gacatcctgg aggagatcaa caaggctggg gtttcaatcc tacttgtaga gcacaatatt
    10261 aaggtggccc tgtccctggc tgaccgggtc tacctgatgg gcaaggctca catcgggttc
    10321 gcgggaaccg tggcggaatt ggaggcgaac ccggagacca aagcgaaata cctagaagtc
    10381 tgaaatgatc aatatgtgtg aacctaaata atcctaaatt tcgtaaccat ttggtctttt
    10441 gaaaatctga aagatctcct ttcatcctct ttttaaagga ggaaaagccg aatcttccgc
    10501 tgtacatctc cttcaagccc ttctctgttc gaaaaagggg cgggagggat ttctgggcaa
    10561 gccttttcaa gacgctaaag tcttattaat tttattctgg taaacccccg gctctgccgg
    10621 gggactcaaa gagtttgaca tttccgggat gtcggagttg tggcccgggg gcaggggtct
    10681 ggggttcaaa aagcatcggc cgggcgatcc ctattcctgg aggctgggac tggcttccgc
    10741 ttgagccggc ggcccgatcg ggcgaccggc cggtcgcccc tacaaggaca aaaccttcca
    10801 cggggttgcg ggaatggctg gccgacccgg cctatgaaat gaaggatgcc ttatggtttt
    10861 tgaacccgag acccttgccc cccggcctgg cattggcgtc caggggatgg ccacctttag
    10921 gtggcgctca tttcaatccg ctttgagcgg ttcaggggta tttcatgcct ccacctctgg
    10981 tggaggtcat gaatttggaa atctataaaa gaagggtaat gaatttctcg gcgcatattg
    11041 aacgaagctg caaaatgaat cctcataaga cctgtgtggt tcagggagac cttcgactta
    11101 cgtatgcgca gatggcggaa agagggaacc gtttggcacg ggctttggtt gaaaggggac
    11161 taaaacccgg tgatcgagtc gctatcttcc tgaccaattg ctttcaattt gccgagatgc
    11221 tttatgcgat tgccaaaatc ggagggattt tcctcaatct gaacttccga ctacgtgaag
    11281 aagagacctc ttatattttg cgcaatgccg agccaaaaat ccttattttg ggggatcgat
    11341 atgttgaact catcaacaat atccggccca gactctcctt catagaatgt tttatagtca
    11401 tgggacagca tccatcggat atgcaggatt atgaatccgt tctctcccat caatctccgg
    11461 ccccatatcc ctgtatttct gttcaagggg atgatacggc ttgcctcata tacaccagtg
    11521 gaaccactgg acttcctaaa ggagctatgt taacccatgc caatctcgta actcctttga
    11581 aagataaata cttcatccct gcgggtacgt tgctcctcaa tgtccctatg taccacatcg
    11641 ccggggttct cagtaccctg gttccactct atagaggcga tacccaaatc gtgcttcccc
    11701 aattcgagac aggcattttt ctagagatcg ttgaaaagga gaaggtagcc accacctacc
    11761 ttgttcccac catgttacag ggcattctgg accaccccga ctttcccaag agagacctca
    11821 gttcgttaaa agcgattgga tatggcgctt ctcctatgcc ggtaaaactt ctcctgcggg
    11881 ctaaagatct cctcaaagta gattatacga attacttcgg cttgacggag tccacgggga
    11941 tcgtttctgt tctatcccct gaagaccatc gattagaggg aggcaaagat gagctgggaa
    12001 aaaaaacacg gcggctcgca ggaattggcc attcaattcc cgagggcgaa gtccggattg
    12061 tggatgacca ggatcgagat gtgccgattg ggaaggtagg cgagatcctt gcccgaggga
    12121 aaaagatcat gaaaggctat tggaataatc ccaaagctac ggaagaaacc cttcgggggg
    12181 gatggctgca tacgggagac ctggcccgta tggatgaaga gggatatctt taccttgccg
    12241 gaaggaaaaa ggatatgatt attcgtggag gggaaaacat ctatccggtc gaaattgaaa
    12301 atgttctgca tagccatccc aaagtattgg aggcggccgt cattggagta cccgatgaat
    12361 attgggggga aatcgttaaa gcagtcatta tcctgaggcc gggtgagcgg tctacttcag
    12421 aggaaatcat tggatactgc cgggaaagat tagcaagcta taagaaaccg gcgatcgtgg
    12481 aatttgtcga cgccctgcct aaaaatgcga tgcagaaagt attgaaaaac gtccttcgag
    12541 gtgaggcgcc aaaatagaga aattgccaat attggcgaaa gatgtaagcg gagcctcaag
    12601 gtaaaaaagc cgagttgatg gaaaaatccg ggagttggat tttcttttcg gccttcaaga
    12661 ggaatttgcc atctttagga aggcctcctt attatttgtt tgaccaagac agagtaaagg
    12721 aggtgctgcc catcttttaa ttttaagagt tcagtttgtc aaggccgttc agcgaaaatc
    12781 gcgatgatgg agcttggtac ctggattctc agattaacca acggaaagga ggaagagcaa
    12841 tggattcgat tagtaaaata cgttccagtt taatcttcat ggggttcgtt tttgcattct
    12901 ctgttttctt attcggtatg ccccaggcaa tggccttcga gcccctcaag ggggacctct
    12961 cgaaatatga cccgaacaag ctggatcttc ccaccggaga tgtcgtcaag atcgccatgt
    13021 gggatcaggt ttcgggggtg aacgcctatc tcgcggaggc ttactccgcc ttgcttggat
    13081 ttgttgccca ggacatcaac tcccagggcg ggatcaaggt ggacggcaag atgaagaagg
    13141 tctcctttgt cctggcggac actcagggga ggccggaccc gggcaaacgg gcggccgaaa
    13201 aagcgatcct gcaagacaag gttaacgtat ttgcgggagt cgccggaagc cacgttgcca
    13261 aggtgggcca gggaacagcc aaagaatata agaccatttt catgaacgtc tccgcatact
    13321 ccgatgaact catggacctc ccggactgga acgagtatgt ctttcgcacc tgcgggactt
    13381 ccgccaccgt cggcaagagc ctggccattt tttatgaaaa acgacccgaa acgaagtttt
    13441 acatcttggc ccaggactat ctctggggac actcctctgc cggcgccttc aaaaaggcct
    13501 taaaagaatc taagcccaaa gcccagattg tcggcgagga gtactttccc gtgtacacca
    13561 aggatttcgc gccctactta gaaaaggtga gggcatccgg agccgaggtg attgttcaaa
    13621 tggcctgggg agccgacaat gagaacctga tcaagcaatc gagacaactc ggtttgaaaa
    13681 gccccttgaa tccggaatat tacattccca tggcgagtcc ctttctggat gacacccgtc
    13741 ccttggaggt tatcggcggc ccggccggcc gaggcttggt cctgtgcacg gattttcagc
    13801 tggatcgcag ggtgccgaac gttaagaaat taaccgacat atggaccggc ctgtggaaga
    13861 catggaaagc gccctataat acaaggcttt accaatggcc caacggcggt tggtatagaa
    13921 atctcctgtc ctactactgg tatcttcagg ttgtccaaaa ggccggcagc accgatacaa
    13981 agaaggtcat cgccgcctgg
//
LOCUS       DASPRR010000353         3858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2021624, whole genome shotgun sequence.
ACCESSION   DASPRR010000353 DASPRR010000000
VERSION     DASPRR010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021624"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VEL68_13320"
     CDS             <1..379
                     /locus_tag="VEL68_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HXZ36992.1"
                     /translation="LNLGNQHSLGALIQGNRVWGIFEHHTGRMTREKLKGILERFPTK
                     AVTNEEIFQDQGHGCAYRPGYSRKKGFRFVAVTGPRRSMVEGLGYYLAAPYGNMMLAG
                     CFGLIAALKAKLNTGCQARGQGV"
     gene            603..2621
                     /gene="ligA"
                     /locus_tag="VEL68_13325"
     CDS             603..2621
                     /gene="ligA"
                     /locus_tag="VEL68_13325"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXZ36993.1"
                     /translation="MNEAQAKKKIEKLREEVNFHNNRYYVLDDPVISDAEYDRLMREL
                     EGLENQFPHLRTPTSPTQRVGAPPLDKFEEVRHAVPMLSLANAFEEAEVREFDDRLKR
                     FLKNTREIEYCTELKIDGIAVELIYENGHLITGSTRGDGFVGENVTQNLKTVKAIPLT
                     LIPQKGEFLPSRLEVRGEVYLPLKAFRELNAKRERSGESLFANPRNAAAGSLRQLDSS
                     ITAQRPLDIFCHGIGQFTGAAIGSQWELLDSLQRWGFKVNPNRRICANIDGVFDYYRE
                     MDELREKLPYEIDGVVLKVNSFRLQETLGTIARSPRWALAFKFKPKQVTTKVLDIAVN
                     VGRTGALTPAAVLEPVRVGGVTISSATLHNQDEIDRKDVRIGDTVVVQRAGDVIPEVV
                     RVIPEKRTGKEKPFRLPDRCPVCGSEVDRPEGEAVARCTGIACPAKLKETIIHFASRD
                     AMNIEGLGAKIIEQLVDRGLIKDYADLYKLTLDQMLTLERMGPKLAGNILTAIQNSKK
                     TTLDRLIYALGILHVGEHIAKLISQEFSTAEELAEASHERLTGIRGIGGEIASSMVKF
                     FQQKGNQKVLQKLNDLGVEYPKRPTRPKARDLKLQGKTFVFTGGLKTLSRGEAESKVE
                     SLGGRASSSVSKKTDFVVAGEDPGSKLEKAKILGVKILSEDELLEMLR"
     gene            2669..2947
                     /locus_tag="VEL68_13330"
     CDS             2669..2947
                     /locus_tag="VEL68_13330"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXZ36994.1"
                     /translation="MAKSRARVIVKGIVQGVNFRYYTQRQAIRSNITGWVRNLPDGSV
                     AAIFEGDEQDVEAMVQWCRHGPPSAQVTELIAQPEEFRGEFSSFSVKY"
     gene            complement(3144..3410)
                     /locus_tag="VEL68_13335"
     CDS             complement(3144..3410)
                     /locus_tag="VEL68_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36995.1"
                     /translation="MEVGDRIKFSFGGEEKEGVVVKIFPKKVYLRVDFPRHKGKLIVR
                     SLAALEGKIPQGKKKEKAKKEKKTKASPSKKKISEEKKEEEGKG"
BASE COUNT          899 a         1034 c         1078 g          847 t
ORIGIN      
        1 tctgaatctg ggcaaccagc atagcctggg cgccttgatc cagggaaatc gcgtttgggg
       61 aatctttgag catcacaccg gacggatgac ccgagagaag ctcaaaggga tcctggaacg
      121 ttttcccacg aaggcggtca cgaatgagga aatcttccag gaccagggtc atgggtgcgc
      181 ctatcgtcca ggatattccc gcaagaaagg atttcgcttt gtggcggtca ccgggcccag
      241 gcgttcaatg gtggaaggat taggttatta cttggcagct ccctacggga atatgatgct
      301 ggccggctgt ttcggcctca tcgcggcttt aaaggccaaa ttaaacacag ggtgccaggc
      361 ccgggggcaa ggagtttagg cgcaaaaatg atgagggggc ttccatttca tggccggtcg
      421 ggcaaaccgt tcgaatcccc gttgaaggtg tctttttctt gtagggagag cggccggtcg
      481 ctcgatcgaa ccgccgaatc ctgaggaagc aggttgaaag ctcgagagat attattggct
      541 ccttttgaat tttgatttac ctctaagatc tccgtgtctc tgtgatgaaa aggtagtttc
      601 gtatgaacga agcgcaggcc aagaagaaaa tcgaaaagct acgggaagag gttaacttcc
      661 acaacaaccg ctactatgtc cttgacgacc cggtcatatc cgatgctgaa tacgaccggc
      721 tcatgcggga gctggaaggc ctggagaatc aatttcccca tctgcgcacc cctacttcgc
      781 cgacccagcg ggtgggagct ccccccctgg ataaatttga agaggtccgg catgccgttc
      841 ccatgctcag cctggccaat gcctttgagg aagcggaagt tcgcgaattc gacgaccggc
      901 tcaaacgctt tttgaaaaac actcgggaga ttgaatactg cacggaactg aagattgacg
      961 ggatagccgt ggaactgatc tacgagaacg gtcacttgat taccggttcc acccgggggg
     1021 acggtttcgt gggagaaaac gttacccaga acctcaagac ggtcaaggcc atacccttaa
     1081 ccttaatccc ccaaaagggg gagtttcttc cctcccggct cgaagttcgg ggagaagtct
     1141 atctccctct caaagccttc cgcgagctga acgccaagag ggagcggagc ggcgagtctc
     1201 ttttcgccaa ccctcggaac gcggctgctg gctccctccg ccagcttgat tcctcgatca
     1261 ccgcccaacg ccccctcgac atcttctgcc acggcatcgg gcaattcacc ggggctgcca
     1321 tcggcagcca atgggagctt ctcgattccc tgcaacgatg gggattcaag gtcaacccca
     1381 accgcaggat ttgcgcgaac atcgacgggg tcttcgatta ctaccgggaa atggatgaac
     1441 tccgggaaaa gcttccctac gaaatcgacg gcgtggtcct caaagtaaac tccttccgtc
     1501 tccaggagac tcttgggacc atcgcccgaa gcccccgctg ggcgctggct ttcaagttca
     1561 agcccaagca ggtgaccacc aaggtgctgg atattgccgt caacgtggga agaaccgggg
     1621 cgctaactcc cgcggcggtc ttggagccgg tccgggtggg gggagtgacc atcagcagcg
     1681 ccacccttca caaccaggat gagatcgacc gaaaggatgt tcggattggc gacaccgtgg
     1741 tcgttcagcg ggccggggat gtcatccccg aagtggtccg ggtgattccc gagaagcgca
     1801 cggggaaaga gaagccgttc cgcctccctg accggtgtcc ggtttgcggg tcggaagtgg
     1861 accgcccgga aggagaggcc gtggcccgct gcacgggcat cgcctgcccg gccaagctca
     1921 aggaaacgat cattcacttc gcttcccggg atgccatgaa tatcgagggg ctaggggcta
     1981 agatcatcga acagctggta gaccggggcc tgatcaaaga ttacgccgac ctctacaaat
     2041 tgaccctcga ccaaatgctc accttggaga gaatgggtcc caaactggcg ggcaacatct
     2101 tgactgccat ccagaacagc aagaagacta ccctggacag gcttatctat gccctgggaa
     2161 tccttcatgt gggagaacac atagccaaac tgatcagcca ggaattttcc accgcggaag
     2221 aactagccga ggcctcccat gaaaggctca ccggcatccg cgggatcggg ggagaaatcg
     2281 cctcctccat ggtcaaattc ttccagcaga aggggaatca gaaggtcctc cagaagctga
     2341 atgacctggg ggtggaatat cctaaacggc cgactcgccc caaggccagg gacctcaaac
     2401 ttcaggggaa gacctttgtt ttcaccggcg gattaaagac gctgagccgg ggcgaggcgg
     2461 aatcgaaggt tgaatcccta ggaggacggg cctcctcatc ggtcagcaaa aagaccgatt
     2521 ttgtggtggc cggagaagac cctgggtcca agttggaaaa ggcaaagatt ttaggcgtaa
     2581 agattttatc ggaagatgaa ttattggaaa tgttgagata aaactatttc aggagagagg
     2641 gaatggggtg aggggggagg tcgatcgaat ggccaaatct cgagccaggg ttattgtgaa
     2701 aggaatcgtt cagggagtga atttccgcta ctatacccag aggcaggcta tccgcagcaa
     2761 cattaccggc tgggtgcgga atcttcccga tgggtctgtg gcggccatct tcgaaggtga
     2821 cgaacaggat gtggaggcca tggtccagtg gtgccgtcat gggccgccgt ccgcccaggt
     2881 caccgagttg atcgctcagc cggaggaatt ccggggagaa ttctcttctt tttcggttaa
     2941 atactgaccg gggagagggt gacccgcccc ctctcttctg gaccctaaaa gatgaccgcc
     3001 ttcactcttc ttctgatctg tgtgggagct caaatcctga ttgccgcggc tctgggaatc
     3061 tttctctttc gccatttttc acggcaaaga aaagaaaagg agaaaaagga cctcaaaccc
     3121 tgagaaggcc cccccattgt cggttatccc ttcccctctt cttccttctt ttcttcgctt
     3181 atcttctttt tggaaggcga tgctttggtc ttcttttcct ttttggcctt ctccttcttc
     3241 ttgccctggg gaatcttccc ctccagcgcg gcaagggaac gaacgatcaa tttccccttg
     3301 tgcctcggga aatcaaccct cagatagacc ttcttgggaa agattttgac taccacgccc
     3361 tctttctctt ccccaccgaa agaaaatttg atccgatcgc cgacctccat ctccgcttcc
     3421 tccttgtgat tggatcattt ttaaatacca tttccgcccc tgccgggcaa gggaaaattg
     3481 gctgggggtg gaggaaacaa gccaaagtga atcccctttt aaatttcctt cgggtaaacc
     3541 accgactctg gcgaagaggt cataaagttt ctttcttttt ggatacctaa gttgtagccc
     3601 tggggcaggg caaggctcct acatctttga ctctcacctg atttctggaa agtgaggcgg
     3661 gcaagcctga taagccccct tgctttcgtc gggaaccatg gataaaatgc ttgacagggt
     3721 aggagaagac gatgaagaac cacatggatt gggaacggcg cgagcgggaa aggaaagtga
     3781 tggagattgt ccgttccttt ctcctcgcct ttctggcctt tcgccggctg catggagaat
     3841 accaaaatgg ctctcttc
//
LOCUS       DASPRR010000354         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2338128, whole genome shotgun sequence.
ACCESSION   DASPRR010000354 DASPRR010000000
VERSION     DASPRR010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338128"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VEL68_13340"
     CDS             <1..546
                     /locus_tag="VEL68_13340"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ36996.1"
                     /translation="YGFIVALFYYRELKWGDIPRVLVESAVTTGFVGLLLGLASVFGW
                     VLTVEQAPLRLANLILSISTSQWVFLLLVNILFLIAGCFLNATAILIILVPILLPMVH
                     KFEVDLIHFGIILIANLGIGYVTPPVGTCLYVACGISKQPLAEVIKPLAPYLVVMVIM
                     LMLITYVPWVSLVIPNLVYGK"
     gene            677..1090
                     /locus_tag="VEL68_13345"
     CDS             677..1090
                     /locus_tag="VEL68_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXZ36997.1"
                     /translation="MVHYEDCIVFLLAKAYQRAHSAFKVKLTPFGITPVQHLILAVLG
                     EEDFLSPAEITDRVAMDGATLSGVLDRMTEAGLIKKEENPQDRRSIRVSLSPRARRMW
                     EELAEQRRSINEELTAKFSLEEKLLLKRMLKDLKG"
     gene            1174..1368
                     /locus_tag="VEL68_13350"
     CDS             1174..1368
                     /locus_tag="VEL68_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36998.1"
                     /translation="MGEVVHTSRIKIVREKGPTRRAVIEGFNEPIYYGVHGGIKKFYK
                     VEPEKEHAATLDHIVAATAG"
     gene            1369..1641
                     /locus_tag="VEL68_13355"
     CDS             1369..1641
                     /locus_tag="VEL68_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ36999.1"
                     /translation="MMGTLAGGLAGKRIPTAEDHYWAEVEGDIEDVNGILKITRIRVR
                     YHLKIPKEKAKDAQEAFSTYLVRCPAAQSVIGCIRIEDKLIIEEEV"
     gene            1653..>2474
                     /locus_tag="VEL68_13360"
     CDS             1653..>2474
                     /locus_tag="VEL68_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HXZ37000.1"
                     /translation="MATLTEQVKEIIRSYGGARMGITTRETLVGGPPSTDLTYILPEA
                     KSAITFALPLNRKFIRPFLAKEDRLSHERDNLQVNLLATGLAAHIAKHLEQKGFPSQG
                     VVSNESYRDEKPWGRLGMNPDLSHRYLAVRSGVGWFGFSGNVITPEYGAAVILGTCVT
                     SAELEPTDPLPPEDNTCNRCKLCLAACCSGMMDPKEEARVTLGGKEFTYAKRQNYLRC
                     EFVCGGMTGLHPSKKWSTWSPGRFFIPKEDGAFREAMIKGIKAYDRRPRMEGGFPH"
BASE COUNT          570 a          672 c          709 g          523 t
ORIGIN      
        1 tacggtttta tcgtggccct tttctattat cgggaactga agtgggggga tatcccgcgg
       61 gttctcgttg aatctgcggt caccaccggt ttcgtgggtc tcctcctcgg gctagccagc
      121 gtcttcggct gggtcctgac cgtcgaacag gcgcccttga gattggccaa cctcatcctg
      181 tccatatcca cgagccagtg ggtctttctt cttctggtta atattctctt cctcatcgcg
      241 ggctgtttcc taaacgccac ggctatcctg atcattctgg tccccatcct tttgcccatg
      301 gtccataagt tcgaggtgga cctgatccat ttcggcatca ttctgatcgc caacctaggg
      361 atcggatatg tcactccgcc cgtgggaacc tgcctctatg tggcctgcgg gatctccaaa
      421 cagcccttgg cggaggtgat caaacccctc gccccctatc ttgtcgtcat ggtgatcatg
      481 ttgatgctga tcacctacgt cccgtgggtc tcactcgtga tccccaacct ggtttacggg
      541 aaatgattca cacggggcgc ccctttctct agagggctcg ctcaacccgt gcactcttaa
      601 aaaactcttg acagagggga tgagattata ttattttgta cacaaattaa tttcgaggcg
      661 ggggggacct ccatccatgg tccactatga agactgcatt gttttccttt tagccaaggc
      721 ttaccagcgg gcccactcgg cttttaaggt gaaactcacc ccattcggaa ttaccccggt
      781 tcagcacctg atccttgccg ttctggggga agaggatttt ctttctcccg cggaaattac
      841 ggaccgagta gccatggatg gggccacatt atcaggggtt ctggaccgga tgacggaggc
      901 gggcctgatc aaaaaggagg agaacccgca ggaccggcgt tcgatccggg tgtccctatc
      961 gcccagggcc aggaggatgt gggaagaact ggccgagcag agaagatcca tcaacgagga
     1021 attaaccgcg aaattttcgc tggaagaaaa actcctgctc aaacggatgc tgaaagacct
     1081 gaagggatga tcgtttgcct acgaaggaaa agagcgggtt caaggttcga gttccaggtt
     1141 tcgtaaaacc cattaacgaa aaaggaggga ggcatggggg aagtggtgca tacgtcccgt
     1201 atcaagatcg taagggagaa aggccccacc cggagagcag tgatcgaagg atttaacgaa
     1261 cccatctact acggggttca cggggggatc aaaaaattct acaaggtgga accggagaag
     1321 gaacatgcgg ccaccctgga ccatatcgtg gccgctaccg ccggctgaat gatgggcacg
     1381 ctggccgggg ggctggcggg aaaaagaatc ccgactgccg aagaccatta ctgggctgag
     1441 gtggaggggg atatcgaaga tgtgaacggg atcctaaaga ttacccgaat tcgggtccgc
     1501 taccacttga agattcccaa agaaaaagct aaggatgccc aggaagcctt ctctacctat
     1561 ctggtcagat gtccagcggc ccagagcgtc atcgggtgca ttcggattga ggacaaattg
     1621 atcatcgagg aagaagtcta ggaggaggaa cgatggccac cttaacggaa caggtaaaag
     1681 agatcattcg ctcctatggc ggggcgcgga tgggcattac cacgcgggaa acgctggttg
     1741 gaggcccgcc ctccaccgac ctgacctaca ttctacccga ggctaagtcg gccatcactt
     1801 tcgcccttcc gctcaacaga aaattcatcc gccccttcct ggccaaggag gatcgcctga
     1861 gccacgaaag agataacctc caggtgaacc tactagctac tgggctggcg gcgcatatcg
     1921 ccaagcatct ggaacaaaag gggttccctt cccagggggt tgtgtcgaac gaatcctacc
     1981 gggatgagaa gccatgggga aggttgggaa tgaacccgga cctctcccac cgctacctgg
     2041 ctgtccgttc cggggtcggg tggttcgggt tttcgggaaa cgtgatcacc ccggagtacg
     2101 gagcggcggt catcctgggg acctgcgtaa cctccgcgga actggagcct acagacccat
     2161 tacccccgga agacaatacc tgcaaccgct gcaagctctg cctggccgcg tgctgctccg
     2221 gaatgatgga ccccaaagaa gaagcccggg tgaccctggg agggaaagag tttacctatg
     2281 ccaaaaggca gaattacctt cggtgcgaat tcgtctgcgg cgggatgacc gggctccatc
     2341 cctcgaagaa gtggtccacc tggtcgcccg gccgattctt catccctaag gaagacgggg
     2401 ccttccggga ggccatgatc aagggaatca aggcctatga tcgacggccc aggatggaag
     2461 ggggtttccc ccat
//
LOCUS       DASPRR010000355         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_735524, whole genome shotgun sequence.
ACCESSION   DASPRR010000355 DASPRR010000000
VERSION     DASPRR010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735524"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VEL68_13365"
     CDS             complement(<1..792)
                     /locus_tag="VEL68_13365"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXZ37001.1"
                     /translation="MKTREDRITELRAAVVPIKLAEPFAAGRWVLEACINVVSAVRSA
                     SGSMGFGYAFVFREIDGQTILSAINGLREVVINQDPLDVMGLHGDLRAAINFLGAGGA
                     GMSAVGAIDLALWDLRGRILGIPVHALLGSSKTKIPAYASGGPFNKDLKALEREVRGY
                     VDTGFKAVKIKLPPDIHGACQRIAACHSWVGDRVKMLYDSNQQQSVKDAVTIAKCCVD
                     HNAYWYEEPFPYWQLQFSADLRGQIPCRLALGETLYGEAPFYEAVK"
     gene            890..1015
                     /locus_tag="VEL68_13370"
     CDS             890..1015
                     /locus_tag="VEL68_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37002.1"
                     /translation="MEASPDPGSRFAFRERDIYKEFEGKLWSAGLVEKIRALQKK"
     gene            complement(1063..2151)
                     /locus_tag="VEL68_13375"
     CDS             complement(1063..2151)
                     /locus_tag="VEL68_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009835663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ37003.1"
                     /translation="MKITDVRTMLVNIPFEKPLRTSIHSFESAGCVLVFIDTDEKITG
                     ESYVFSLNVARLEVIKAMILSLKPEVVGEDPHYTEKIWQKIWQGINFFGHKGITLFGL
                     SAIDVACWDIKGKAARSPIYKLVGALRDKTPVYASGGLWLSQTIDELVKEAKDFIAQG
                     FRFMKLRIGKPRIEEDVERVRAVRQAIGPDMGLMVDANQAFKVDHAIKLGRKLEEFNL
                     VWYEEPVAAYDLEGSARVAAALDVPIASGETDYTRYGIRRMLETKAADILMPDLARMG
                     GYTDFLKAAHMAEAYEVPVSPHIFSEQSLQIMGMIPNGTFLEHMPWFAPLFREKMEIT
                     NGMIEIPNRPGTGFTFDPEKIEKYRIRG"
     gene            complement(2286..2954)
                     /locus_tag="VEL68_13380"
     CDS             complement(2286..2954)
                     /locus_tag="VEL68_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020054734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXZ37004.1"
                     /translation="MEIGKITRPAKKLIEGFRQLATSTIGNALDDLQVSGVIQNIKPI
                     CTGFHFAGGAVTVKEVTGVLGTYTNADFTLGQVIDSAQEGDVIVIDNGGQQVSTWGGI
                     AAFAAKKRGVAGLVVDGGVRDLDEIREFDFPVFSRHVVPTSGKGRVKLLSMNTVVKID
                     GIRVRPGDIMVGDGTGIICVPIEIAEDVLTMAKKMDEQDKQGIEEIRRGLSFTEALRK
                     FAKI"
     gene            complement(3025..3567)
                     /locus_tag="VEL68_13385"
     CDS             complement(3025..3567)
                     /locus_tag="VEL68_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ37005.1"
                     /translation="MKTEVRLSGSGGQGMILAGIILAEAAGIHDGKNVVQTQSYGVEA
                     RGGASRAEVIISDEEIVFPEITRPDILLAMNQPSLDKFLGSLKGDGILILDSTHVKSP
                     ANTQSKVYGIPLTELARKAGGVIVSNIVALGALVGLTRVASEEAMRKAVLSRVPKGTE
                     ELNMRALDLGLQAAQNALGK"
BASE COUNT          758 a         1072 c          913 g          825 t
ORIGIN      
        1 ctttacggcc tcatagaatg gcgcttcccc atagagagtt tcccccaagg ccagccggca
       61 gggaatctgc ccccggaggt ccgctgaaaa ctggagctgc caatagggaa acggttcctc
      121 gtaccaatag gcgttatgat ccacgcaaca cttggcaatc gtaaccgcat ccttcaccga
      181 ttgctgttgg ttggaatcat agagcatttt cacccgatcc cccacccagg agtgacaggc
      241 cgcgatccgt tggcaggctc catggatatc ggggggaagt ttaatcttga ccgccttaaa
      301 ccccgtatcg acataccctc gcacttccct ctctaaagcc ttgaggtctt tgttgaaggg
      361 accgccgctg gcataagctg gaatcttagt tttgctggaa ccgagcaggg catggacagg
      421 gatacccaga attcttcccc ttaagtccca caaggctaaa tcaatggccc ccacggcaga
      481 catgcctgcg ccacccgccc cgagaaaatt gatggccgcc cgcaagtctc catgaaggcc
      541 catgacgtcc aaggggtctt ggttgatgac gacttcgcga aggccgttga tggcggatag
      601 aatcgtctgc ccgtcgattt cccggaaaac aaaggcatat ccaaacccca tggaacccga
      661 ggcgctccgt actgcggaca caacgttgat gcaagcttcg agaacccatc gccctgcggc
      721 aaatggctcc gcgagcttaa tgggaacgac ggcagcgcgc aactcggtaa tccggtcctc
      781 tctggtcttc atagcatcct ccagcgaaaa aaagtgtcag tgtcggtgtc agcaaaggca
      841 accattatcc cgcaacccct ttgcccgttt tgatttcagc cagggtctga tggaagcctc
      901 cccggaccca gggagccgct tcgcattccg ggagcgggat atttacaagg aatttgaagg
      961 caagctctgg tcggccggtc tggtagaaaa gatccgggcc ctccagaaga agtaattctc
     1021 cggaatcaga agggggtggg actccctccc cccttctatc ctttatccgc gaatccgata
     1081 tttttcgatc ttttcgggat cgaaggtgaa cccggtcccc ggccggttgg ggatctcgat
     1141 cattccgttt gtgatttcca ttttttcccg gaacagcggc gcaaaccagg gcatatgctc
     1201 caggaatgta ccgttgggga tcatgcccat gatctggagg ctttgctccg aaaaaatgtg
     1261 cggggaaacg ggaacttcat aggcttccgc catatgggcc gccttcagga agtccgtgta
     1321 accccccatc cgggccaggt ccggcatcaa tatatctgca gccttcgttt ccagcatccg
     1381 gcgaattcca taacgggtgt aatctgtttc cccgctggcg atgggaacat ccagagcggc
     1441 ggccacccgg gccgaacctt ccaggtcata ggcggccacg ggctcctcgt accatacgag
     1501 attgaactct tcgagcttcc ggcccagctt gatggcatgg tccactttga aggcctggtt
     1561 ggcgtctacc atgaggccca tatcgggccc gatcgcctgg cggaccgccc ggacccgttc
     1621 tacatcctcc tcgatccggg gtttgccgat gcggagtttc atgaaccgga acccctgggc
     1681 aatgaagtct ttggcttctt tcaccagctc gtcgatcgtt tgggaaagcc agagccctcc
     1741 gctggcgtaa accggtgttt tgtctcggag cgcaccgaca agcttataga tcgggcttcg
     1801 cgccgctttc cctttgatat cccagcaggc cacgtcgata gctgagaggc cgaagagggt
     1861 gatccctttg tggccgaaga aattgattcc ctgccagatc ttctgccaga tcttttccgt
     1921 gtagtgcggg tcctcgccaa ccacttccgg cttcaaactc aggatcatgg ccttgatgac
     1981 ttccagcctc gccacattca aggagaacac atagctctct ccggtgatct tctcatccgt
     2041 gtcgatgaac acgagcacac agccggcaga ttcaaaggaa tggatcgagg tccggagcgg
     2101 tttttcaaag gggatattga ccagcatcgt gcgcacatcg gtaattttca tcgttcacct
     2161 ctttttttaa gaaaaagtgt gaatgtgggg gtgagtaaaa caatcattag gccctaacgg
     2221 ccttattcat attctcttcg agacgaatac tatttttccc ttcacactca tgactcacac
     2281 ggtcttcata tttttgcaaa cttccgcagg gcttccgtga aactcaagcc ccggcgaatt
     2341 tcctcgatcc cctgcttatc ctgttcgtcc atctttttcg ccatggtcag cacatcttca
     2401 gcaatctcaa tgggcacgca gataatgccg gttccatccc ccaccatgat atctcccggt
     2461 cgcacccgga tgccgtcgat tttcacgacc gtgttcatgg agagaagttt gacccgtcct
     2521 tttccggaag tggggaccac atggcgggaa aagaccggaa aatcgaattc ccggatctca
     2581 tccaggtccc ggacccctcc atcgacgacc aacccggcca ctccgcgctt tttggccgcg
     2641 aaggcagcga tccctcccca ggtggaaacc tgctgcccgc cgttgtcgat cacgatgaca
     2701 tccccttcct gggccgaatc gatcacctgg cccaacgtaa aatccgcatt ggtgtaggtc
     2761 cccaaaaccc cggtgacctc tttgaccgtc accgcaccgc cggcaaaatg aaaccccgtg
     2821 cagatgggct tgatgttctg gatgaccccg gagacttgca gatcatccaa ggcattgccg
     2881 atggtgctgg tggccagttg ccggaaccct tcaatcagct tcttcgccgg gcgagtgatc
     2941 ttgccaattt ccatgctttc ctccttaaag caaattctcc atttcttact gattggccat
     3001 ctcagggaag gattggctgc ggacctattt tcccagggcg ttttgggctg cttgcagccc
     3061 taagtccagg gcgcgcatgt tcaattcttc tgttcccttg ggaacccggg acagaactgc
     3121 tttgcgcata gcctcttcgg aagccacccg ggtcagaccc accagggcac ccaaagcgac
     3181 gatgttggag acgatgacgc cgccagcttt gcgggccagt tcggttagag ggatcccgta
     3241 taccttggat tgggtattgg cagggctctt cacatgggtg ctgtccagga tcaggatgcc
     3301 atcccccttc aacgaaccca gaaacttatc cagggaaggt tgattcatag ccaaaaggat
     3361 atccggccgg gttatttcgg ggaaaacgat ttcttcatcg ctgatgatca cttccgcccg
     3421 gctggctcct ccccgggcct cgacgccgta ggattgggtc tggaccacat tcttcccatc
     3481 gtgaatgccg gcagcctcgg ctaaaatgat accggccagg atcatcccct gcccccccga
     3541 gccgctgagt cgtacctcgg tcttcatt
//
LOCUS       DASPRR010000356         9273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2178599, whole genome shotgun sequence.
ACCESSION   DASPRR010000356 DASPRR010000000
VERSION     DASPRR010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2178599"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VEL68_13390"
     CDS             <1..181
                     /locus_tag="VEL68_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HXZ37006.1"
                     /translation="RKRKDGLKMEGHAGALVGYTRTRFIIRNSWGTAWGDKGYAYASF
                     RYAESAFSEAYGVVL"
     gene            complement(292..1671)
                     /locus_tag="VEL68_13395"
     CDS             complement(292..1671)
                     /locus_tag="VEL68_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ37007.1"
                     /translation="MNIVDHMKLFFEPQSVAILGVSRKTGPGAFNILECLQLYGYQGK
                     VYPVNPNAREILGIRCYPSVEDIRGPIELAVISLDRSQVLSAVESCVNAGIKALVIIS
                     QGFADLGKEAKILQDKTRDVARAKGARIVGPNTMGVVNNFHNFTTSSLPLVKFHSPVS
                     TICQTGIFITGFYTLTGPVGKAIDLGNTCDVDFADVLEYYAADPSTQIIVLHIEGIQE
                     GRKFLQAARKVTPHKVVLALKTGRTEMGAQRAASHSGSMAGKDFLYSAAFKQSGIIRA
                     GDSEELSDLVKGFSCLPPPRGNRIALLTYTGGGGVMAVDRMMELNFPLVQLSPKTLEK
                     FSLLNPPWLPLGNPLDMWPSTMKYGARPMYKTYLQGLLEDDGVDAVLCIILSPQLPGQ
                     EYFDVSDEIIEAATRFPDKPIVVWAYGPDLEKTQGKLDSSGRVVILPSAERAIQLLYA
                     LRERRRFLE"
     gene            complement(1814..3637)
                     /locus_tag="VEL68_13400"
     CDS             complement(1814..3637)
                     /locus_tag="VEL68_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXZ37008.1"
                     /translation="MQPVLVAEGKTVPEMFHSRVRLSGDGVSLRYKKLGIWHDVSWKE
                     YAQRVREVCMALLALGLKPGECVSVIGENSPEWVYIDLGTMHAGGTTVGVYATNSWEQ
                     CQYVVDHSESRFYFVENEEQLDKALRFRANVPRMEKIIVWDLKGLKHFKDPMVTSFDE
                     FLAPGKELDRKEPDLFEKRWRAVKSEDLARLIYTSGTTGPPKGAMLTHTNITWMAQAM
                     AEGNPIEAQDEFLSFLPLCHIFEQLFTIFMNIRYGAIVNFIENTDTVTDNMREVSPTV
                     AYGVPRVWEKYFSGIMIMMSDATWLKRLIFQASLKTGKKYAALTTSHQPVPGWLQLAY
                     SVAHFATFRKLKERLGFDRVRLAFSGAAPISPDVLRFFQAIGIPLREGYGMTEGTGVT
                     CANQGDRVKIGTVGQALPGVEVKIAEDGEICFRGGNVFKGYFRNPQATAEVLEPDGWM
                     HSGDVGVLDAEGFLKITDRKKDLIITAGGKNIAPQNIENQLKFSPYINDAVVIGDRRK
                     YLVAIIVIDEDNVVKYAQDNKFQYTTYATLTQHPEINKVIQKEVDAVNKTLANVETVK
                     KFTILSKKLFVEDGEVTPTMKVKRKYINEAFKDAIEGMYRD"
     gene            complement(3715..4176)
                     /locus_tag="VEL68_13405"
     CDS             complement(3715..4176)
                     /locus_tag="VEL68_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37009.1"
                     /translation="MHRLLKTICILLALFFIFQMAFSPGLLAFTIIEKKTFKEGPLHF
                     LLEVQVYGSGSSKKIPLRVNSVKVKIKNERASSKILEVKAVRAYLEPQVFRDLETRGY
                     PVSPGQWVTKYYRFPKEKRPLLGEKGFIQIDFDGFALTFDPKERKFQGPLN"
     gene            complement(4505..5476)
                     /gene="epsC"
                     /locus_tag="VEL68_13410"
     CDS             complement(4505..5476)
                     /gene="epsC"
                     /locus_tag="VEL68_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HXZ37010.1"
                     /translation="MDEKKISLTGDQCEIEIDFVEGYRSQLPRVIEKIIGSIQEERSI
                     SHFNSALIPSKESVTEIIENLKDLLFPGYYRKQEIDTYSLSYQIGSGVNRVFENLASQ
                     IARSIRHECRRLNSLCTRCLDRGQQEALAFLEKIPPLRKVLAGDVQAAYDGDPAAKSL
                     DEIIFSYPCILAITVYRVAHELHVQGVPLLPRIMTEYAHSITGIDIHPGAIMGRNFFI
                     DHGTGVVIGETTEIGDRVKIYQGVTLGALSFPKDSEGNIIRGRKRHPTLEEDVTIYSN
                     ATILGPVVIGARSVIGGNTWITHDLPPDTIVTIEQPGLRYRNGNEEK"
     gene            complement(5545..6402)
                     /locus_tag="VEL68_13415"
     CDS             complement(5545..6402)
                     /locus_tag="VEL68_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXZ37011.1"
                     /translation="MPPPLIIPMEELFSFAFMQRAFLAGLGVSVTCAILGVFLVLRRD
                     AMIGHGLAHVTFGGVALGILLNVAPLSVALAVAILSALGILKLKERAGLYGDTAIGII
                     SSLGMALGIFLVSLAGGFNLDLFGYLFGNVLAIDPREVWVAFLLALAVLATLAFFYQE
                     FLFLTFDSESARASGVRVERLDSLMAVLTAVTVVVGMKVVGILLISALLVIPAAGALQ
                     VARNFKGAMALSAVLAGLSAAGGLIAAFYLDWPPSGTIVLVSGVLFLILFASRQWVKR
                     PGRTGSSQP"
     gene            complement(6387..7142)
                     /locus_tag="VEL68_13420"
     CDS             complement(6387..7142)
                     /locus_tag="VEL68_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HXZ37012.1"
                     /translation="MNPKDPLLSVENVSFRYNGLQVVQDVSFAVFPGDFLALIGPNGS
                     GKTTLIRIILGILKPTRGRVVFMGEEMGRFSQWQRIGYVPQKATHKDPYFPASVREVV
                     AMGLLSRKRFPRFLNRRDETSIDRALDLVDIKDLKDRRIGELSGGQQQRAFIARAIVN
                     RPNALFLDEPTAGVDAETQAHFYDMLAGLNQKEGLTIILITHDIGVITKHINKVACLN
                     QRLFFHGSHEEFCNSSVVEQLLQGEHHLVCHRH"
     gene            complement(7132..8100)
                     /locus_tag="VEL68_13425"
     CDS             complement(7132..8100)
                     /locus_tag="VEL68_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HXZ37013.1"
                     /translation="MKRRDVRLFLLQWGWAFFVFQSIYLWTCPFAQARAKIKVVTTLF
                     PLQEFSKAVGGEKVQADLLLPPGAEAHAWEPKVSDLGKVHKADVFIYIGPAMEPWAHD
                     LLKALKGKESKVVEASAGVQLLEARDFDHPKEGGHSQKHPRDEALDPHVWLDFSLDLK
                     IIDAIAAAYTEKDPGNASQYRTRARAYQSRLEALDQKYQSSLARCRHRRIILGGHSAF
                     AYLAKRYGLQQLALYGVSPNAEPTPKKLTEVINAARNDGVKFIFFEELVNPKMALVLA
                     KEAGIQTLVLHGGANLTREQIEKKVTFLELMEKNLENLRKGLECEP"
     gene            8422..9012
                     /locus_tag="VEL68_13430"
     CDS             8422..9012
                     /locus_tag="VEL68_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXZ37014.1"
                     /translation="MPSWLESLDAALFGLINRGGQNSFFDWFMPFMTDLKNFTYVLVL
                     VGALVLWRERKAGLVFLVFVGLTLAITDPFSSLVLKEWIGRIRPCQVLPGVRLLTDCN
                     TSYSFPSSHAVNIFAAAYFLSQPFKRLAPLFFGIAAIVAYSRIYIGIHYPLDVIGGAA
                     IGLLIAWPMRRVKDEVLTRLHISAAENTKSRAPKTE"
BASE COUNT         2003 a         2545 c         2626 g         2099 t
ORIGIN      
        1 aagaaaaagg aaagacgggc tgaaaatgga gggccacgcc ggtgccctgg taggctacac
       61 ccgtacccga ttcatcattc ggaacagctg gggaaccgcc tggggagaca aaggatatgc
      121 ctacgcctct ttccgctacg cggaatcggc cttcagcgaa gcctacgggg tcgtcctgta
      181 agaaatagtc gattttgggg cgaagcctta tttcgctgtg tcaccaaccc aatctggatc
      241 gtggcaggtt gatcttccgc cctccccctt taaccgggag aaagcctttt tctattccaa
      301 gaacctccgg cgttccctta gggcatacag aagctggatg gccctttcgg cggagggaag
      361 gatgacgacg cggcctgagg aatccagctt cccctgggtt ttttccaggt cggggccgta
      421 ggcccagacg acgatgggtt tgtcggggaa tcgggtggca gcttcgatga tttcgtcgga
      481 gacgtcgaaa tactcctggc ccggaagttg gggagataga ataatgcaaa gcactgcatc
      541 gaccccatca tcctccaaga gaccctgaag gtaagtttta tacatcgggc gggcgccgta
      601 tttcatcgtg ctcggccaca tgtccagcgg gttccccagg ggcagccagg gcggattcaa
      661 aagagagaat ttttcaaggg tctttggaga gagctggacc agggggaaat tcagctccat
      721 catccggtcc accgccatga ctcccccgcc cccggtgtag gtcagtaaag caattctgtt
      781 cccccgaggc gggggaaggc aagaaaatcc cttcaccagg tcacttaatt cttccgaatc
      841 ccctgcccga atgatcccgc tctgtttgaa agccgcacta taaagaaaat cctttcctgc
      901 catggagcca ctgtgggaag ccgctctctg cgctcccatc tcagtcctgc cggtcttcaa
      961 tgccaaaacg actttatgcg gagtaacctt cctggccgcc tggagaaact ttctcccctc
     1021 ctgtattcct tcgatatgga ggacaatgat ctgggtgctg gggtcagcgg cataatattc
     1081 caaaacatcg gcaaaatcca catcgcaggt gttcccgaga tcgatggctt tacccaccgg
     1141 cccggtcaag gtgtaaaacc cggtgatgaa gattccggtc tggcagatgg tcgaaaccgg
     1201 ggaatgaaat ttcaccagcg gaagggaaga ggtggtgaaa ttatgaaaat tgttgaccac
     1261 ccccatggtg ttggggccga cgattcgggc cccttttgcc ctcgcgacgt cgcgggtctt
     1321 atcctggaga atcttcgctt ctttgcccag atcagcgaat ccctgagaga taatgaccag
     1381 ggccttgatc ccggcattca cacaggattc aaccgcagaa agaacctggc ttcggtccag
     1441 ggatatgacg gcaagctcga tgggtccccg gatgtcttcg acagaaggat aacagcggat
     1501 gccgagaatc tcccgggcgt tcgggttgac gggataaacc ttgccctggt agccatagag
     1561 ctgaaggcat tcaaggatat tgaaagcgcc cggaccggtt ttcctcgaga cccccaggat
     1621 ggccacggat tggggttcga agaataactt catatggtca acgatattca ttgcgagtct
     1681 tcaagactca tgcttacaga atcgtacggc aatccctaaa tcccctcagc ctcccttatt
     1741 caatgcggag attcggcccc caacctctaa cctccagcct ctaacctcca acctctaacc
     1801 tccaacctcc aacctaatcc ctatacatcc cttcaatcgc gtccttgaaa gcctcgttga
     1861 tgtatttccg cttgaccttc atggtggggg tcacttcacc gtcttccacg aagagcttct
     1921 tggaaagaat ggtgaacttc ttcaccgtct ccacgttggc cagggttttg ttcaccgcgt
     1981 ccacttcttt ctggatcacc ttattgatct ccgggtgctg ggtcagggtg gcgtaggttg
     2041 tgtattggaa cttgttatcc tgggcgtatt tgaccacatt gtcctcgtcg atgacgatga
     2101 tggccaccag atacttgcgc cggtcgccga tgaccacggc gtcgttgata taggggctga
     2161 atttgagctg gttctcgata ttctgagggg cgatgttctt tccgccggcg gtgatgatca
     2221 ggtccttctt tcggtcggtt atcttgagaa agccttcggc gtcgagcacc cccacgtccc
     2281 cggaatgcat ccatccatcc ggctccagga cttccgccgt ggcttgagga ttcctgaaat
     2341 accccttgaa cacgttccct ccccggaaac agatctctcc atcttcggcg atcttgacct
     2401 caacgccagg cagggcctgg cccaccgtcc cgatcttcac ccggtctccc tggttggcac
     2461 aggtaacccc ggtgccctcg gtcatcccgt aaccttcccg gagggggatg ccgatggcct
     2521 ggaagaagcg gagtacatcg ggggagatgg gagccgcgcc ggagaaggcc agacggacgc
     2581 ggtcgaaacc caatcgctct ttcagcttgc ggaaggtggc gaagtgggcc acagaatacg
     2641 ccaattggag ccagcccgga accggttgat gactagtggt gagggcggcg tattttttcc
     2701 cggtcttgag gctagcctgg aagatgagcc tcttgagcca ggtggcgtcc gacatcatga
     2761 tcatgattcc ggaaaaatat ttctcccaga cgcggggcac gccgtaggcc acggtggggg
     2821 agacctctct catgttgtcg gtcaccgtat ccgtgttctc gatgaagttg acgatggccc
     2881 catacctgat gttcatgaaa atggtaaaga gctgctcgaa aatgtggcac aagggcagga
     2941 aggagaggaa ttcatcctgc gcttcaatcg ggttgccctc agccatggcc tgggccatcc
     3001 aggtgatgtt cgtgtgagtc agcatggccc ctttgggcgg ccccgtggtg ccggaggtgt
     3061 aaatcaagcg ggccaggtct tccgatttga ccgccctcca gcgtttttca aagaggtcgg
     3121 gttccttccg gtcgagttcc ttaccggggg ccaggaactc gtcgaagctc gtcaccatgg
     3181 ggtccttgaa gtgtttcaag cctttgaggt cccagacgat gatcttttcc atgcggggaa
     3241 cgttggcccg gaagcgaagg gctttatcca gctgctcttc gttttccaca aagtaaaagc
     3301 gggactcgga atgatctacc acatattgac attgctccca gctgttggtg gcgtagaccc
     3361 cgacggtcgt ccccccagcg tgcatggtcc ccaaatcgat atagacccac tcgggggagt
     3421 tttcccctat gaccgacacg cactccccgg gcttcaaccc cagggcgaga agggccatac
     3481 acacttcccg taccctctgg gcgtattctt tccaggagac gtcgtgccag atgcccagct
     3541 tcttgtagcg caaggacact ccgtctccgg acaaccgaac gcgggagtgg aacatttcgg
     3601 gaacggtttt cccttcggcg actaataccg gctgcatatc cttcctccga cgtaaaagtt
     3661 cgtccctttt tccgatggtt ctaccgtgaa agtctggaat tcctccccga tggctcagtt
     3721 caagggtccc tgaaatttgc gttccttggg gtcgaaggtg agggcaaagc cgtcaaagtc
     3781 gatctggata aatcccttct cacccagaag gggccgcttt tccttgggga agcggtaata
     3841 cttcgtcacc cattggccgg gagagacggg atatcccctg gtttcaaggt cccggaacac
     3901 ctgcggctcc aggtaggcgc gcaccgcttt gacttccagg attttggaac tggcacgttc
     3961 attctttatt ttgactttga ccgaattgac ccgcaggggg atttttttgg agctgccgct
     4021 gccgtagacc tgaacttcga gcaggaaatg aaggggcccc tctttgaagg tctttttttc
     4081 gatgatggtg aaggccaaaa ggccggggct gaaagccatc tggaaaatga aaaataaggc
     4141 caagagaatg cagatggttt tcaggagacg atgcatggat tcccctcctc ggaatccgtc
     4201 cgatgcgcat cgatttggtt cagcccgttt tagggtgtag actctctttt tcaaaatgcc
     4261 cttccaggaa gcgaataaca ggaattccct gggaaagtca agcaaatttt aaagccatgg
     4321 tactggatac tggttactcg tttctcgtga tcgaatcaag gacccatcct gaaaccagca
     4381 accagtaacg aataaccaac aacccatttt ggcttccgag cgcacggcct cgtggccgtg
     4441 cgcccctact cccccctaaa ccaacttaag cgcacctaag tgcacttaag gcacttgagg
     4501 cactttattt ctcctcattc ccgtttcgat accttagccc gggttgctcg atggtcacga
     4561 tggtatccgg aggaaggtcg tgggtgatcc aggtgtttcc accaatgacc gaccttgccc
     4621 cgataaccac cggtcccagg atggtggcgt tggagtagat ggtgacatcc tcctccaggg
     4681 tcggatggcg tttccgcccc cggatgatgt ttccttcgct gtctttggga aaactcaggg
     4741 cccccagggt gacgccctga tagatcttga cccggtcccc gatttctgtg gtctcgccga
     4801 tcactacgcc cgtgccgtga tcgatgaaaa agttccttcc catgatagct ccgggatgga
     4861 tgtcgatgcc ggtgatcgaa tgggcgtact cggtcatgat ccgggggaga agggggaccc
     4921 cttggacgtg aagctcatgg gcaacccggt agacggtgat ggccaggatg caggggtagc
     4981 tgaagatgat ttcgtccagg ctcttggctg ccgggtctcc atcataagcg gcctgcacat
     5041 caccggccag caccttgcgc agcgggggga tcttttcgag gaaggcaagg gcctcctgct
     5101 ggccccggtc caggcaacgg gtgcagaggc tgttgaggcg ccggcattca tgccggatgg
     5161 agcgggcgat ctgggaagcg agattctcga agactcggtt taccccgctg ccgatctgat
     5221 agctcaggga gtacgtgtca atctcctgct tacggtagta gccggggaaa aggaggtctt
     5281 tgaggttctc gatgatttcg gtgaccgatt ccttggaggg gatcaaggcc gagttgaaat
     5341 gagagatcga ccgttcctcc tggatgctcc cgatgatttt ttcaatcacc cggggaagct
     5401 ggctgcggta tccttcgaca aagtcgatct caatctcgca ttgatcgccg gtcagggaaa
     5461 tttttttttc gtccataaca gcgacttcct tccttgggga aggttctcga ttcggtgaag
     5521 ggtcctgaga accttccccg ggatttacgg ttgagacgac ccggtcctcc ccggcctttt
     5581 gacccattgg cgggaggcaa aaaggatgag gaagaggact ccggagacca gtacgattgt
     5641 ccccgaaggg ggccagtcga gatagaaggc ggcgatcagc ccccctgcag cggagagtcc
     5701 ggccagaacg gcggagaggg ccatggctcc cttgaaattg cgggccacct gcaaagcccc
     5761 ggcggcgggg atgaccagaa gggcggagat cagaaggatg cccacgactt tcatccccac
     5821 gaccacggtc acggcggtta gaacggccat caggctgtcg agccgctcca cccggacccc
     5881 cgaggccctg gccgactccg aatcgaaggt gaggaaaaga aactcctggt agaaaaatgc
     5941 gagcgtagcc agcacggcaa gggccaggag gaacgccacc cagacttctc gcgggtcgat
     6001 ggccaggacg ttcccgaaga gataaccgaa gaggtccagg ttaaagccac cggccagact
     6061 gacgagaaag attcccaagg ccatccccag gctgctgatg atcccgatgg ccgtgtctcc
     6121 gtaaagcccg gctcgctcct tcaatttcag gattcccagg gcggagagga tcgcaactgc
     6181 cagggcgacc gacagggggg ccacattgag gagaatcccc agggccactc ccccgaaggt
     6241 cacgtgggcc aggccgtggc cgatcatggc gtccctgcgc aggacaagaa acacccccag
     6301 gatggcgcag gttacggaaa cgcccaaccc ggccaggaaa gctctttgca tgaaggcaaa
     6361 agaaaagagt tcttccatgg gaatgattaa tggcggtggc aaaccagatg gtgttcgcct
     6421 tgaaggagct gctcgacgac ggaagaattg cagaattcct cgtggcttcc gtggaaaaaa
     6481 aggcgctggt tgagacaggc caccttgttg atgtgtttgg tgatcacccc gatatcatgg
     6541 gtgatgagga tgatcgttag gccctccttt tggttgaggc cggcgagcat atcgtaaaag
     6601 tgggcctgag tctccgcgtc caccccggcc gtcggctcgt ccaggaagag ggcatttgga
     6661 cggttgacga tcgcccgagc gatgaacgcg cgctgctgct ggccaccgga gagttctccg
     6721 atgcgccggt ctttcaggtc ctttatgtcc accaaatcca gggctcgatc gatggaggtt
     6781 tcgtctcgcc ggtttaagaa ccgggggaag cgcttccggg aaagcagccc catggccact
     6841 acctcccgca ccgaagccgg aaaataaggg tccttgtggg tggccttctg ggggacgtag
     6901 ccgattcgct gccactggga aaagcgaccc atctcctccc ccatgaaaac cacccgtccc
     6961 cgggtgggtt tcagaattcc caggatgatc cggatcaagg tggtctttcc cgatccgttg
     7021 ggtccgatca gggccaaaaa gtcacccggg aaaacggcga aagaaacgtc ctggacgacc
     7081 tgcaggccgt tgtaccggaa agagacattc tccacggaaa ggagcgggtc tttagggttc
     7141 acattccaat ccctttcgca aattttccag gttcttctcc atcaattcca ggaaggtcac
     7201 cttcttttcg atctgctccc gggtcaggtt ggcccccccg tgaaggacca gggtttgaat
     7261 tccagcttcc ttggccagaa ccagggccat cttggggttg accaattctt cgaagaagat
     7321 gaatttcacc ccgtcattcc gggcggcgtt gatgacctcg gtcagtttct tgggagtggg
     7381 ttcggcgttg gggcttactc cgtagagagc gagctgctgc aatccgtacc ttttggccag
     7441 gtaggcaaag gcggaatgac cgcccaggat gatgcggcga tgacgacatc gggctaggga
     7501 agactggtac ttttggtcca gagcttccag ccgtgactgg taggctctcg cccgggtacg
     7561 gtattgggaa gcgttccccg ggtccttttc ggtgtaagct gcggctatag catcgatgat
     7621 tttcaggtcc agggagaagt ccagccagac atgcgggtcc agcgcctcat cccgggggtg
     7681 cttttgagaa tggcctccct ctttggggtg atcgaaatcc cgagcctcca ggagttgaac
     7741 ccccgcgctg gcttccacca ccttcgattc ttttcccttc agggctttga gaaggtcgtg
     7801 ggcccagggt tccatggccg ggccgatata gatgaaaaca tccgctttat ggaccttacc
     7861 caggtcgctg accttgggct cccaggcgtg ggcctcggct ccgggcggaa gaaggaggtc
     7921 ggcctggacc ttttcgcccc ctacggcctt ggagaattcc tgcaaaggga acagggtagt
     7981 taccaccttg atcttcgccc gggcctgggc gaatgggcaa gtccaaagat agatggactg
     8041 gaagacaaaa aaggcccatc cccattggag gaggaaaagg cggacatccc ttcttttcat
     8101 atgaccttta ggttggcccc cggggcccgg aatttctcaa aacgtgaagg attccatgtt
     8161 tttataatat cctagcggtt tcccggggga gtttcaagga aattcagccg gtcagaaggg
     8221 aaaagcaagg attctgggct ccttttcaaa ttcaggatgc gttccattct gggaatgtaa
     8281 ggctttccct ctttttaagc attgcaaacc cgtagacaga aagggcggat ttcattaaaa
     8341 taatgatcgt cgtctcttga gtggatgtag aatctcaggg gatatttttt ttctgggaga
     8401 tggatttttc gcgggtcagc catgccctct tggttggaat cactggacgc ggcacttttc
     8461 gggctcatca atcgcggcgg gcagaactcc ttcttcgact ggttcatgcc cttcatgacc
     8521 gatctgaaga actttaccta cgtcctcgtc ctggtggggg ctctggtgtt gtggcgggaa
     8581 agaaaagcag gattggtctt cctggttttt gtcggactta ccctggccat caccgatccc
     8641 ttttccagtc tcgttctcaa ggaatggatc gggaggattc gcccgtgcca ggttctcccc
     8701 ggagttcggc ttttgaccga ttgcaatacc tcgtactctt tcccttcctc ccacgcagtg
     8761 aacatatttg cggcggccta tttcttatcg cagccgttca aaaggttggc cccccttttc
     8821 ttcgggattg cggccatcgt ggcctattcc cgcatctaca tcggaatcca ttaccccctg
     8881 gatgtcatcg gaggggcggc catcggtctt ctcatcgcct ggcccatgcg tcgggtgaaa
     8941 gatgaggtct tgacccggct ccatatatcg gccgccgaaa acaccaaatc ccgagcaccg
     9001 aaaaccgaat aaaaatacag ttgggacgcg gatgaacgca gatcatcagg atttttaaaa
     9061 aagtggctac tcgaagaaaa gtgacatcag gtcctgggtt ctttttttgc gccaatctgc
     9121 gaaaaaatgc atccacacaa atttaagaaa gggacaaaaa atgcggagca acgctgagat
     9181 tgttgtcgag gccctggaaa aggcgggaac gaaatatatc ttcggatatc caggggggca
     9241 gaatgcaagg tttatcgaag ccatgcacgg tag
//
LOCUS       DASPRR010000357         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1860595, whole genome shotgun sequence.
ACCESSION   DASPRR010000357 DASPRR010000000
VERSION     DASPRR010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860595"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            534..1700
                     /gene="alaC"
                     /locus_tag="VEL68_13435"
     CDS             534..1700
                     /gene="alaC"
                     /locus_tag="VEL68_13435"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HXZ37015.1"
                     /translation="MEEFHRIRRLPPYVFSIVDDLKMKARRRGDDIIDLGMGNPDLPT
                     PPHIVEKLIETIKNPRTHRYSASRGIPKLRAAIAGWYARNYGVEVDPDSEAVVTIGAK
                     EGISHLMLATLGTGEVVFVPTPSYPIHTYSVVIAGGDFRAVPLAKGRDFFEDLQTATR
                     QIWPQPKMMVISFPHNPTTQVVELPFFEKIVDFARAHNMMVVHDLAYADLVFDGYKAP
                     SFLQVPGAKEVGVEFFTLSKSYSMPGWRVGFCVGNRRMIHALTRIKSYLDYGIFTPIQ
                     VAGIIALNDDQSCVEEVRKTYQERRDALVDGLRRVGWKIEKPKGTMFVWAEIPEKFKE
                     MGSLEFSKLVLQVGKVAVSPGIGFGDTGDTHVRFALVENTHRIKQAVKGIKKLF"
     gene            1816..3135
                     /locus_tag="VEL68_13440"
     CDS             1816..3135
                     /locus_tag="VEL68_13440"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXZ37016.1"
                     /translation="MDRINIGLIGFGTVGTGLVKILQENGKVLEKRLGVPMVLKRIAD
                     QDVTRDRGVKINPALLTTQVSEIIDDPEVPIVVELIGGLEPARTIILQAIGRGKSVVT
                     ANKALLAVHGTEIFKAADEAGVDVGFEGSVGGGIPIIRSLKEGLVANKIQILFGILNG
                     TSNYILTEMTQKGLKFSEVLKKAQELGFAEADPTLDVGGMDTAHKLCILLSLAYGIQV
                     PLEDIYTEGITGITPMDIEYAKELGYQIKLLAIAKSDGGPVEARVHPTLLPADHLLST
                     VGGPFNAIFVKGDAVGSTLFYGAGAGMMPTGSAVVSDLVDICRNMRMGVHRRVPLLSY
                     QREHLREAEIKPMQDISAPYYLRFTVVDKPGVLSRISGILGDLDISILSVIQKGRKSE
                     KAVFIYMVTHEAREKNMRKALQVIDQLPVIVDQTVFIRIEDPSNLGG"
     gene            3315..>3927
                     /locus_tag="VEL68_13445"
     CDS             3315..>3927
                     /locus_tag="VEL68_13445"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HXZ37017.1"
                     /translation="MADYPLSELQGKTPLQVAKTPNLDQMAKKGVLGLVRTVPLAMSP
                     GSDVANLSIFGYDPALYFTGRAPLEAAAMGVKLNPEDVAFRCNLVTLGSRNGRETMDD
                     FSAGHISTGEARKIIQEVGEILGTDEFHFFPGVSYRHLLVWRNGERGLKARTTPPHDI
                     SGKEIAEFLPRGSGQEEILSLMAKAKKVLTDFPVNQERLKAGKK"
BASE COUNT          877 a         1022 c         1144 g          884 t
ORIGIN      
        1 aacgggttct ttccttgccc accccgagtt cttaaaaaag agtccttcga aaataagatc
       61 tctggaaaag aggggggatc tcatggggtt cagggcaaga aatttggatc atcatcctga
      121 ggcggtggtt cttccattgg atcgggcctg tcctggggga ttgaaacgga aacatgcttt
      181 ggctctcttt tctgcttgga ggtatctcct ctcaggcctc tgcctgcgga acatggggag
      241 gagtttcatg gaaggataag cgcccgccga gcctgatgat ccaaattccc ccaccctgaa
      301 ccccaacaag cacgaaaaac tgggggtggg caaggggagg ggaaccacaa tggacagccc
      361 caatgaacag caaaaatctt gacaaagggg caggggttgg gtagttttta agtagggtgg
      421 gatgagcctc cggacctcgt tttgaggttc tttatttttt atggattctt agaggctcct
      481 caaagaccct taggattttc acctatattc tttcaccttt ggggtgttcc gccatggaag
      541 aattccaccg tatcaggagg cttcctccgt atgtcttctc catcgtagac gacctaaaaa
      601 tgaaggcacg gcgccggggc gatgatatca tcgacttggg gatgggcaat ccggaccttc
      661 ccactcctcc ccacatcgtg gaaaagctca tcgagaccat caagaatccc cgcacccatc
      721 gctattccgc ctcccgggga attcccaagc tccgggccgc catcgccggg tggtacgcca
      781 ggaattacgg cgtggaggtc gatccggatt ccgaagcagt cgtcaccatc ggggccaagg
      841 agggcatctc tcatctgatg ctggccaccc tagggaccgg ggaggtggtt tttgtaccga
      901 ccccgtccta tcccattcat acctattcgg tggtcattgc cggcggggat tttcgtgcgg
      961 ttcccctggc caaagggcga gatttcttcg aggacctgca aaccgcaact cggcaaatct
     1021 ggccccagcc caagatgatg gtgatttctt ttccccacaa tcccacaacc caggttgtgg
     1081 aacttccctt ttttgaaaag atcgtggact tcgccagggc gcacaacatg atggtggtcc
     1141 atgacctggc ctatgccgat ttggttttcg acgggtataa ggccccgagt ttcctccagg
     1201 tcccgggagc caaggaggtg ggggtggagt tctttaccct atcgaagagt tacagcatgc
     1261 cggggtggcg ggtcggtttc tgcgtgggaa accgcagaat gatccatgct ctgacccgaa
     1321 tcaagagcta cctggattac gggatcttca ctcccattca agtggcgggg atcatcgctc
     1381 tgaatgatga tcaaagctgc gtcgaagagg tccgcaaaac ttaccaggaa cggagggatg
     1441 ccctggtgga cggcctgcgg cgggtcggct ggaagatcga aaagcccaaa ggaaccatgt
     1501 tcgtgtgggc ggagatcccg gaaaaattca aggagatggg ttccctggaa ttctccaagc
     1561 tggtcctcca ggtggggaag gtggcagtct ccccgggaat cggcttcggg gatacgggag
     1621 acacccacgt gcgtttcgcc ctggtggaaa acactcaccg gatcaaacag gcggtcaaag
     1681 gaattaaaaa gctattttaa ccatagagaa cctggagttc ccagaggaga aaaatcagaa
     1741 acaggattct ttccctttgt ttttgtgctg tggtctctgt cctatcagtg gttcactctg
     1801 tttttgggag atgacatgga tcgaatcaat atcggactca tcggatttgg cacggtcggc
     1861 accggattgg tgaagatcct gcaggaaaac ggaaaggtgc tggaaaaacg actgggcgtt
     1921 cccatggtct tgaaacggat tgccgaccag gatgtaaccc gggaccgggg ggtcaaaatc
     1981 aatccggctc tgcttacgac ccaggtctct gaaattatcg atgacccgga ggtccccatt
     2041 gttgtcgagc tgatcggcgg cttggagccg gctcggacta tcatccttca ggccatcggg
     2101 cgcgggaaaa gtgtggtcac cgccaataaa gcccttctcg cggttcacgg caccgagatt
     2161 ttcaaggccg ccgacgaggc tggggtggac gtcggtttcg aagggagcgt ggggggcggg
     2221 attcccatta tccgttcgct caaagagggc ttggtggcca acaagatcca gattctcttc
     2281 ggcatcctga acgggacctc taattacatt ctgacggaga tgacccagaa gggattaaag
     2341 ttctccgagg ttttgaagaa ggcccaagaa ctcgggtttg ccgaagcaga ccccactttg
     2401 gatgtggggg gcatggacac ggcccataag ctttgtatcc ttctttctct ggcttatgga
     2461 attcaggttc ctctggagga catctatacg gaaggaatca ccgggatcac ccctatggac
     2521 atcgagtatg ccaaggaact ggggtaccag atcaagctcc tggccattgc caagtccgat
     2581 ggagggccgg tcgaagcccg ggtccatccc accctcttgc ccgccgatca cctcctttcc
     2641 acagtgggcg gccctttcaa cgccatcttt gtgaagggcg atgccgttgg gtccacgctc
     2701 ttttacgggg ctggcgcagg catgatgccc acggggagcg ccgtggtgag cgacctggtg
     2761 gacatctgtc ggaatatgcg gatgggggtc caccgaaggg tgcccctgct ttcttaccag
     2821 agggagcatt tgcgggaggc ggaaatcaag ccgatgcaag acatctccgc cccttattac
     2881 ctgcgcttta cggtcgttga caaacctggg gttttatccc gcatatcggg aattctgggc
     2941 gatttggaca tcagcatcct ttcggtgatc cagaaggggc ggaagtcgga gaaggctgtt
     3001 ttcatctata tggtcaccca cgaggcgcgg gaaaagaaca tgagaaaggc cctgcaggtc
     3061 atcgaccagc tgccggtcat tgtggaccag acggtcttta ttcgtattga agacccttcg
     3121 aatctggggg gatgaccgcg agtccagttt cgagttccag gtttcaagtt tcgagtgagt
     3181 ctcttgatcg gaggcaggag cgacgaaacg cttggcctca gccttttccc tttatcctcc
     3241 gacctctgac ccattccggt gtttgaaagg ctttaacctt gggaagaaag tacgcggtac
     3301 tttgcggaga tgggatggcg gattaccccc tttccgaatt gcagggaaag actcccctgc
     3361 aagtggccaa aactcccaac ttggaccaga tggcgaaaaa gggggtcctc ggcttggtgc
     3421 gaacggtccc tctggcgatg tccccgggta gcgatgtggc caacctttcc atcttcggtt
     3481 acgatccggc tctgtacttc acgggcaggg cacctctcga agcagcggcc atgggggtca
     3541 agctgaaccc ggaagacgtg gccttccgct gcaacctggt cacccttggg tccaggaacg
     3601 gccgtgaaac catggacgat ttcagcgcag gccacatttc caccggggag gcccggaaga
     3661 tcatccaaga ggttggagaa attctgggta ctgatgaatt tcattttttc cccggggtga
     3721 gctaccgtca tctcctggtt tggcgaaacg gagagagagg cctcaaggcc aggaccaccc
     3781 caccccatga tatttctgga aaagagattg ccgaatttct tccccgggga agcgggcagg
     3841 aagaaattct ttccctcatg gcgaaggcga aaaaagtgct taccgatttt ccggttaacc
     3901 aagagcggct gaaagcgggg aaaaagc
//
LOCUS       DASPRR010000358        21201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2338610, whole genome shotgun sequence.
ACCESSION   DASPRR010000358 DASPRR010000000
VERSION     DASPRR010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 21201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21201)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21201
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338610"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..2414
                     /locus_tag="VEL68_13450"
     CDS             249..2414
                     /locus_tag="VEL68_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ37018.1"
                     /translation="MKKYHLPLMPVIWGITCLIIGILVAIGLYSYSSAEKAMADQFNQ
                     QQLVLARQGAQGMEKYLGTLRQTLMVLTRTPEARNLKKEGDEENLKALWGISGGAFDF
                     LFQVDGGGKLLSSYPSKELEGLAGEFFHLRSHLEKAHTTGKSVVVHLGSFPEEKPPDP
                     SSRFGSILILSPLFQGPDPAGFLGAGVDFKKIYEQFVHPISSGARGSSWMIDGEGRFI
                     AHEDPSFMGKDAFSARKDRDPGLSSEQIDRIMREKMLAGQAGTGEYASGWHLGEQGRI
                     KKLVAYAPVRLDGQVWSVAVVVPYSDVTQLVWGSFRNSAVLILIMACILLAGTYVGHK
                     INQERVRAAEKVKWGEEILRSQNRLQAFFDGAPDAIAIVDRSYRILMVNKTAQTWYKR
                     GLEDFTGKPCHQEFQNRPDLCINCPAEESFRTGRPAFRERASLVAGGTKYYLQIFTFP
                     LRDRNGEVVEVVEYVKDVTAEKELQQQIIQSERLAVVGRMAANVAHEIKNPLGTIVLN
                     AELLGEELEKLGSKRNAESQELLGIIKSEVDRLLEVVEEYLQFARLPKIKVEEGGVNE
                     VISDLFLFLREELEERKILLVEELDPQIPPVQLDPKQLRQALLNIVKNSFEAMPEGGK
                     LTLTTAQRDGKVQITISDTGKGISPENQDLVFTPFFSTKHGGTGLGLSITSHIVKEHR
                     GTIGFESYEGLGTSFTIRLPISSSPLVASGGEKPAAARG"
     gene            2442..3902
                     /locus_tag="VEL68_13455"
     CDS             2442..3902
                     /locus_tag="VEL68_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ37019.1"
                     /translation="MDLPEPRNILLVDDHKSFRDTLAKILSAEGFRVFPADDGEAALD
                     ILRQEFIHLVLTDLKMPKMDGVELLKVAKTIRPETEVIIITGYGTVDTAVTAMKDGAY
                     DYIPKPFKPREVLKLVRKALEKQSLVLENRLLQERIKDFQKVEKIVGRSPAMKHILEI
                     VAQVAPSSATVLIQGESGTGKEIIGQAIHDLSPRAKNPFIKVSCAALPETLLEAELFG
                     YERGAFTGAIARKEGRFELANGGTLFLDEIGEVSPTVQVKLLRVLQVGEFERLGGTKT
                     IRADVRIVAATNMNLLEAVERKTFREDLYSRLNVITLTLPSLRAREGDIPLLAHHFLE
                     IFKKKNNKTVKGFTQEAMEVMLQYSWPRNVRQLENAIERAVVLTRADMIAPGDLPPEI
                     LKAVDLAAEEPILMDEKNLSIPLGTPMEMIERRVIEETLRYSRGDKNLASKILGISAR
                     TIYRKMEEDKKEGEKREDDREGDLEDSQKINDNLAK"
     gene            4288..4788
                     /locus_tag="VEL68_13460"
     CDS             4288..4788
                     /locus_tag="VEL68_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ37020.1"
                     /translation="MEKKTILVVDNGETIRETLVKILEREGYRVLAAEDGQAALDVLK
                     ENEVNLILSDVCMPRMDGNKLLKQAKAIHPDVEVILMTGHGKTEMGLEALRAGAFDFI
                     HKPFTKLALNKTIKQALEKQAMAAEMRFLKERVRELLGEIMDLVGEPAVHRMGYSRSL
                     RADSTH"
     gene            4978..6954
                     /gene="acs"
                     /locus_tag="VEL68_13465"
     CDS             4978..6954
                     /gene="acs"
                     /locus_tag="VEL68_13465"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXZ37021.1"
                     /translation="MAEEKKTITSMMEEKRTFPPSKEFSSKAHIKSLEDYKKMYERSI
                     KDPDGFWGEQAKTLEWIKKWDKVLDYSFGDNLYIKWFQGGKINVTVNCLDRHLKTQRK
                     NKIALIWEGEPEGDARKYTYQQLYEEVCKFANVLKKKGVKKGDRVTIYMPMIPELPIA
                     LLACARIGAIHSIVFGGFSADSLKDRILDANASVLITTDASYRSGKVIGLKTNADQAL
                     ASCPGVKTVIVYNRTNTKVDMKAGRDFWWHEEMAAKDIKPECPPEVMDAEDPLFMLYT
                     SGSTGKPKGVLHTQAGYILFVMTSFKWIFDYKDEDIFWCTADIGWVTGHSYIVYGPLA
                     AGATSLMFEGVPNYPKPDRFWATVEKYKVNIFYTAPTALRAMMRDGDQWPLGRNLSSL
                     RLLGTVGEPINPEAWMWYYKVIGKEKCPIVDTWWQTETGGILITPLPGAWPIKPGSAT
                     KPFPGVAAAVIREDGSPASPNEGGYLVIKKPWPGMMRTIYGQHERFKETYFVQFPGLY
                     FTGDGARVDEDGDFWLMGRVDDVINVSGHRIGTAEVESALVSHPAVAEAAVVGMPHDI
                     KGQGIYAFVTVKAGQKKSDELKKTLVAHVRKEIGPIATPDKLQFADGLPKTRSGKIMR
                     RILKKIAAGEISELGDTTTLADPAVVQTLVSERQ"
     gene            7035..8285
                     /locus_tag="VEL68_13470"
     CDS             7035..8285
                     /locus_tag="VEL68_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HXZ37022.1"
                     /translation="MSDQKSAAPPEAGQNRGWIVTFSGTGINLALGVLYAWSVISKQI
                     TKEWGWNETQAALPYSVAIAVFAFMMVPAGRMQDKLGPRLVATLGGIFCGIGFILASL
                     GQSLTGLVLGFGILAGAGIGFGYASATPPAVKWFPPARTGLIAGLVVAGFGLASVYIA
                     PLANYLLSAFGIQSTFLILGIAFLLAVVLLSQLLKNPPVGYKPAGNPAPAKAGSAPKA
                     TAVAADYEWTEMVRTPQFYLLWIMFVFGAGAGLMIIGKLAKIVDLQAGIKAGFIFVAM
                     LAIGNAAGRIVAGVLSDKIGRTWTMFAVFVFQAVLMFLLRGLDTYGTLFLASVLIGFN
                     YGANLSVFPSATKDYFGLKNFGVNYGFVFTAWGVGGILGPMLSGQIFDAEKNFNSAYL
                     IAAVCLLIAAGLTFVTRAPKAKAR"
     gene            complement(8444..8722)
                     /locus_tag="VEL68_13475"
     CDS             complement(8444..8722)
                     /locus_tag="VEL68_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37023.1"
                     /translation="MKKSTIAITVSIILIGLASHAFAGEILGYPIVAKQAKFYSIILG
                     VIAASVSMVAISYLCFWLVDLKKRAMVPKRVEKAAFAGELVAAAQSAK"
     gene            9138..11963
                     /locus_tag="VEL68_13480"
     CDS             9138..11963
                     /locus_tag="VEL68_13480"
                     /inference="COORDINATES: protein motif:HMM:NF028510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HXZ37024.1"
                     /translation="MKSFKDWKIRSKLTAFTLLLALLPMGVAAVVSLQKFTEDLKQAY
                     ESDLDHIVTNIRAMCRAQQELLQNKLTSDLQMAHWVFFRNKRKIDISREQIVEFRAED
                     QFTKEVIRVRVPLWSIDDQEMTRDYVLVDQVQQLVGGTCTVFQRIPGDRLMRISTNVL
                     RSDGSRAVGTYLPAASEVSRTILRGETYRGRAFVVNDWYITAYEPIFDDRKEVIGALY
                     VGVPEQRAVALKTAIKSTKIGKTGYAFIMDSSGRLVVHPAKEGETILEAKDSAGFEYI
                     KEMIRLAPQLGETEVGTLRYPWANVELGESTPRMKINKYVYFRDWDWIIAAGSYEEEI
                     FEGVARTKFFITLVALGTVALAILLAVMLSRVLTKPLQELTEVTARMAGGDLTQKVKL
                     SGGDEIGTLSHSFNRMADQVQNYTRNLEEIVQTRTREIQEKEEEYRNLSRLLNSVLES
                     STEYSIMATDPNGVILQYNSGSVKLFGWSKEEVIGKMRISRTFWKDDRSRGLIRVISR
                     KVEEEGMTEYQVERVRKDGSLFTAHAIVTSLKDPSGKTLGFVEIARDITEKLALEKEL
                     WKTKDQVENIVQSSVDAIVTTDPKGKITFVNRAFEEMVGRSGEEIIGLPIYQFYQDGI
                     TEARKIMSILREKGSLKNHETTALKVGGTVPILTSASLLRDEKGQIIGTLGVFKDLTE
                     KKKLEEELQRTQAHLIQVGKMRALGDLVAGVAHELNNPLMAADTFLHVIRERLSPEDE
                     NLKRLELIQKCHERIAKIINHLREFSRQSEFDFRPLNVTEPIENALMITGQQMINHGI
                     RIVKEFQPDLPKIRGDANQLEQVFLNLISNARDAMEKAERKKELTIAASLLRHNGWND
                     VEVIVKDTGNGIPPENLDKVFEPFFSTKEVGRGTGLGLSICYGIIEAHGGRIEVESRV
                     KEGTTFRVFLPVLSSHLGTALEWTVET"
     gene            12020..12385
                     /locus_tag="VEL68_13485"
     CDS             12020..12385
                     /locus_tag="VEL68_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ37025.1"
                     /translation="MGKKILVVDDDELVLIAIQELLTPLGFSVTAQSGGQEALESVSR
                     GRFDLVILDVSMPEITGFEVCQKIRQMESYTDIPILMLTARSGEEDKQRGMEAGANLY
                     LPKPISPKRLIALVEEALK"
     gene            12476..13804
                     /locus_tag="VEL68_13490"
     CDS             12476..13804
                     /locus_tag="VEL68_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ37026.1"
                     /translation="MEHVMGKAESLLREWKGDSYTFGFEVLGRTGDIVAAHGKRALVI
                     VADLGQGWMAGTLQTITDSLKARGVETEIIIGAGPNAPREDVYRLALHVSRTRPDVIL
                     AVGGGSTIDAAKAAGVLATLSPETVKKALLAPDALASTIDPYFGVGNVTKVKGIAGKP
                     LLPLVAVQTAASSGAHLTKYSNITDLVTGQKKLIVDEAIVPQAAVFDYRVTLGSPMDL
                     TLDGGLDGIAHAWEVFMGATGQAYYEKMKEVAKTCLALIVFGLPKVKTDPGDRNARHS
                     LGLGTDLGGYSIMIGGTSGPHLGSFSLIDVLTHGRACAILNPYYTVLFFSKIQDQLGV
                     FAEILKEAGFIKAKTEGKKGRALAMMVARGMIQFSKALGFPATLADAGVSKAHIKRMI
                     NAAKDPQLKMKLQNMPTPMEAERGDVDKLMRPTLDAAFSGDLDLIPAVAV"
     gene            13977..15401
                     /gene="larA"
                     /locus_tag="VEL68_13495"
     CDS             13977..15401
                     /gene="larA"
                     /locus_tag="VEL68_13495"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HXZ37027.1"
                     /translation="MRPDLLETLKRVKAKGWKKVRVEFTGGPLVISVPPDCVELTMKR
                     ADVLKNPARDIQKALDQPCAGPTLEEIIGRKGKDADQLKVAITISDITRPVPYQGKSG
                     ILPPLLKKLHSLGIRRENILIIVGTGTHRGSTEKEKAEMLGKEIVSAYQILDHDCEDE
                     SILVSVGQTPTGTQVHLNRLFHQADVKIATGLTESHFMAGASGGRKAVCPGLVDLRTI
                     QKFHSPEFLESPCATNLVFEGNPCHEEALAVAKAVGIDFTITVTLDKDMRMTGVFAGD
                     LEKVLEFAVEKIKTYVSIPVEEEFDIVLTHGGYVGRNHYQTAKAAVGAIPAVKEKGVI
                     IIAADNRDPEPIGGPEYKTLTHLLKLQGPDRYVEVLKSPSWKFTKDQWEPEVWGKVLR
                     KVGDEGLIYCTPHIPEETFSMLPGVSGYEFLTPAGKRKSRAKKAEEMVQNTIFFYYQT
                     YREKSISPRVAFIREGPYAVPVGS"
     gene            complement(15535..16335)
                     /locus_tag="VEL68_13500"
     CDS             complement(15535..16335)
                     /locus_tag="VEL68_13500"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXZ37028.1"
                     /translation="MIYRNRVKEAIQKSGFALGTFIQTASPENAEIAAACGFDFLILD
                     MEHGSFGMDQLVHLIRGVQIGGATPMVRLPDHSETNIHKVLDAGATGIVLPGVSTAEQ
                     AGRIAHAARYAPVGTRGACPRVRATAHGIHPWEKHVQWSNQNVMVWLIIETLEGFQHL
                     EEIVKTPGIDAVAFGAFDLSQEMGFAGRTDHPEVKRKIERGITTALGNNVAVHMHLFE
                     ASPAEIRESAKHWVGLGAKILTCMTDRRILSTGLKETFSSLASIRDLR"
     gene            complement(16430..17590)
                     /locus_tag="VEL68_13505"
     CDS             complement(16430..17590)
                     /locus_tag="VEL68_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ37029.1"
                     /translation="MDFTFTTDQVQLRHRARDFAEKEIEPFAEELDAAYDYHAEGINR
                     IRKSGLYAYVVPEAYGGKGISSVNLCIVREELAKISTFADEAFIMQGLGSNPIVLFGN
                     ENQKKKYLPPLVDGTRMANFCLTEPSSGSDVAGIQATARREGDFYLLNGRKWYVSRPG
                     HTDISVVFAKTDPGAGGKGISAFIVDREQSSYGVRTERLTFECNIGEVLLQDTRVPGE
                     NRLGEEGQGMRIALGNLDIYRPTVGAAALGMGWRALSLALEYAQKRPMFGRVLSDFQV
                     TQFKLAEMKVSLDAASLLVYRAAWLADNLREKTTLEASAAKYFATETAQKVVDQSLQI
                     HGGIGLHRKSRIENLYRAVRAPRIYEGASEIQLLVIGRELMRFQGAGHDQRL"
     gene            17885..18889
                     /locus_tag="VEL68_13510"
     CDS             17885..18889
                     /locus_tag="VEL68_13510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37030.1"
                     /translation="MEWRRFRLVGKGSRAFFLTVATLFLVCGTSAPRSVFAQEKYPVK
                     PINFLIGFPAGGTTDVCARPLVNAASKILGQPIVVVNKPGGASAVAVATLKNEKPDGY
                     TIGILGSGAVLSQHMRKVPYDTAKDFTPIMQYAVYLYGLVVQSESPWKTFNEFIEYAR
                     NNPGKIRYSTAGPGSPQHLVMERLALKEKIKWTHIPFPGGGPAVTALLGGHVEATSQT
                     TEWKAHVESGRLRLLAVYGEKRMIDFPNVPTLLELGYDISAASLISIAGPKGISPQIV
                     EILHGAFKKAMEDPDFVKASRQLDQPTVYRSPEDLGKHLVKMNEEVGGLVRSLGLREE
                     "
     gene            19065..19703
                     /locus_tag="VEL68_13515"
     CDS             19065..19703
                     /locus_tag="VEL68_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ37031.1"
                     /translation="MEKKKEGKKEGTGKKGISRRAFLQFVGAGAVVSASAGKGVAATP
                     PETAEPSEKIRVSLFVNGRRRRLLVEPRWSLLYVLREKMGLTGTKVGCERGECGACTV
                     LINGHPRYACLTLTVEVEGEEITTLEGLMEGEELGAVQKAFVEHDAFQCGYCTPGQIM
                     AAEGLLRSNPDPTLEEVRLGMSGNLCRCGVYPNIFRAVRRAAELKRNMRGGR"
     gene            19700..>21201
                     /locus_tag="VEL68_13520"
     CDS             19700..>21201
                     /locus_tag="VEL68_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXZ37032.1"
                     /translation="MSAAEYRDESGQSLRPWKDTTIIGKPMPRVDGYERVSGAAVFPS
                     DTSLPGMLYGAILRCPHAHAHVKSIDTRKAEKMPGVRVVITGKTPGCGVDWTYTTPTA
                     MVKTKLFDPHLRHEGEEVAAVAAETPYQACDAIKAIKVEYEVLPFVVDPKKALEGQAP
                     ALYEGGNRVGKTQTYERGDVTRGFAEADVVLEETYHTACELHTPIELHGCVAKWDGPR
                     LTVWEPTQGVFAVQAGIAQVLGLPLANVRVIGHYVGGGFGSRLTAGKYDIVAALLARK
                     TGRPVKLFLTREETYLCVGNRPPDEMRLKAGVKKDGTLTALEYSALGTGGAYPGSGTS
                     NVDWLVRDLYTCPNVRTDTTDVYINAGPARPFRAPGHPQCAWALEQMMDALAEAIGME
                     PVELRLKNIPSHSQARGNIPYTTTGLKQCLEEGARAFGWKEARQKAAESRRNAGHIRR
                     GVGMAGGLWVVGGGRPPVTVVAKLFADGSVNLNMGASDIGTGTKTVMAMVV"
BASE COUNT         4929 a         5801 c         6145 g         4326 t
ORIGIN      
        1 tgctttggtt ctcttttctg cttggaggaa tctcctctca ggcctctccc tgcggaacat
       61 agggaggggt ttcatggaaa gataagcacc cgccgagcct gatgatccaa attcctccac
      121 cctgaacccc aacaagcacg aaaaactggg ggtggcaagc gccgcaggag ctttgacttc
      181 ggtttccgag ttcgctataa ttccagctaa tgcccaacca agccattatc ccccgcttag
      241 aagaagggat gaagaaatat cacctccccc tgatgccggt gatctggggc atcacctgcc
      301 tgatcatcgg aatcctggtg gccatcggac tctacagtta ttcctctgcg gagaaggcca
      361 tggccgacca attcaaccag cagcagctgg tcctggcccg gcagggcgcc caggggatgg
      421 aaaaatattt gggtaccctc cggcagaccc tgatggtcct gacccggacc cccgaagccc
      481 gcaatctgaa aaaagagggc gacgaagaaa acctgaaggc tctttgggga atatccgggg
      541 gggcttttga tttcctcttc caggtcgatg gcggggggaa attgctttcc tcctatccat
      601 cgaaagagct ggaggggcta gccggggaat ttttccatct tcggtcccat ctggagaagg
      661 cacacaccac gggtaaatcg gtggtggttc acttgggttc tttcccggag gagaaacccc
      721 ccgatccatc ttctcgtttc gggtccatcc tgattttgtc tcctcttttc cagggcccgg
      781 accccgcagg ctttctgggt gccggggtcg actttaaaaa aatctacgag caattcgttc
      841 accccatcag ctccggtgcc cgggggtctt cctggatgat cgatggggaa gggagattca
      901 tcgctcatga agaccccagc ttcatgggaa aggacgcttt ttccgcgcgc aaagaccgag
      961 accccggcct ttcatcggag cagatcgacc ggatcatgcg agagaagatg ctggctggcc
     1021 aagcaggcac gggcgaatat gccagcgggt ggcatctagg agagcaaggg aggatcaaga
     1081 aattggtcgc ctacgccccg gtccgcctgg acggccaggt ttggtccgtg gccgtggtcg
     1141 tcccctactc ggacgtgacc caattagtct gggggagctt ccggaattcg gccgtcctca
     1201 tcctgattat ggcctgcatt cttttagccg ggacttatgt ggggcacaag atcaaccaag
     1261 agcgagtccg ggcggcggag aaagtcaagt ggggcgagga aatcctgcgc agccagaatc
     1321 gccttcaggc gttctttgac ggggccccgg atgccattgc catcgtggac cggagctacc
     1381 ggatcctcat ggtcaataaa accgcccaga cctggtataa acgcggcctg gaggatttta
     1441 ccggcaaacc ctgccatcag gaattccaga atcgcccaga cctgtgcatt aactgcccgg
     1501 cggaggaatc cttccgcacc ggccgtccgg ccttccggga gcgcgccagc ctggtggccg
     1561 gagggaccaa atactacctg cagatcttca cctttcccct ccgcgaccgt aacggggagg
     1621 tagtggaggt cgtggaatac gtcaaagatg taaccgccga gaaggagctg cagcagcaga
     1681 tcattcagtc ggagcgtctg gccgtggtag gccgcatggc ggccaatgtg gcccacgaga
     1741 tcaagaatcc tctggggacc atcgttttga atgccgaact cttgggagaa gaattggaga
     1801 agctcggctc caagagaaac gcagaaagcc aggagcttct gggcatcatc aagtccgagg
     1861 tggaccgtct tctcgaggtg gtggaagaat accttcagtt tgcccgcctg ccgaaaataa
     1921 aggtggagga gggaggggtc aacgaggtga tctccgatct tttccttttc ctcagagaag
     1981 agttggagga gcgaaagatt ctcttggtgg aggaactgga cccccaaatc cccccggtcc
     2041 agctcgaccc caagcagctt cgccaggctt tgttaaacat tgtcaagaac tcctttgagg
     2101 ccatgcccga gggggggaaa ttgaccctta ccacggctca acgggatggg aaagtgcaga
     2161 tcaccatcag cgatacgggc aaggggatct ccccggaaaa tcaggatctc gtttttaccc
     2221 cctttttcag caccaagcac ggaggcacgg gattgggcct ctccattact tcccacatcg
     2281 tgaaggaaca ccgggggacg atcggttttg aaagctatga ggggctggga acctccttca
     2341 ccatccgcct gccgatttcc tcgtctccgc tggtggcctc ggggggagaa aagccggcgg
     2401 cggcccgggg ataaggaccc aagcgatccc agaggagttt catggacctt ccagaaccca
     2461 gaaatattct tttggtggat gaccacaaat ccttccgcga cactctggcc aaaatcctga
     2521 gcgcagaggg attccgggtt tttccggccg acgacgggga agcagccctg gatatcctgc
     2581 gccaggaatt cattcacctg gttttgactg atttgaaaat gcccaagatg gatggagtgg
     2641 aactcttgaa ggtggccaag acgatccgcc cggagacgga ggtgattatt atcacgggct
     2701 atggaacggt ggatacggcg gtgacggcca tgaaggacgg ggcctacgat tacatcccga
     2761 agccattcaa gccgcgggag gtcctcaaac tggtccgaaa ggcattggag aagcaatccc
     2821 tggtcctgga aaaccgcctt ctccaggagc ggatcaaaga tttccaaaaa gtggagaaga
     2881 tcgtgggccg cagtccggcc atgaagcata tcttggaaat cgtggctcaa gttgccccta
     2941 gttcggctac cgtcctcatc cagggagaga gcggaacggg aaaagagatc attggccagg
     3001 cgatccacga cctgagcccc agggccaaga atccctttat caaggtaagc tgtgcggccc
     3061 tgcccgagac cctgctggag gcagagctct tcgggtacga acggggggct tttaccggag
     3121 ccatcgcccg caaagaaggc cgtttcgagc tggccaacgg gggaacccta tttttggacg
     3181 agattggaga ggtgagcccc acggtccagg taaagctcct gcgggttctc caggtgggag
     3241 aatttgagcg tctgggaggc accaaaacca tccgagccga tgtccgaatc gtggccgcca
     3301 ccaacatgaa cctccttgag gcggtggaac ggaagacatt tcgggaagac ctttactcgc
     3361 ggcttaatgt gatcacccta acccttccct ccttgcgtgc gagagagggg gatattcccc
     3421 tcctggcaca ccactttctg gagatcttca agaagaagaa caacaagacg gtgaaaggtt
     3481 tcacccagga agccatggag gtcatgctcc agtattcctg gccgagaaac gtgcgtcagc
     3541 tggaaaatgc catcgagcga gcggtggtgt tgacccgagc agacatgatc gcccctggag
     3601 acctgccccc cgaaattctg aaggctgtgg atcttgcggc cgaggagcca attttaatgg
     3661 acgagaaaaa cctatcgatc cctcttggca cccccatgga gatgatcgaa aggcgggtca
     3721 tcgaggagac gctgaggtac tcccgggggg ataagaatct ggcgtccaag atcctgggga
     3781 tttccgcccg gacgatctat agaaaaatgg aagaggataa gaaagagggc gaaaaaaggg
     3841 aagatgaccg cgaaggcgat cttgaagata gccaaaaaat aaatgacaat ttggcaaaat
     3901 gaccttcttt tgtatcttaa tttgccattt tattctggct ttttatggca aattgccaaa
     3961 ttgtcttctt tttcccaata aaccccaaaa ggccgccatc ccttttccga aattctaata
     4021 aaaaattaat aattttatat ccttacaagt taaatccatc attctttcca tccttctttt
     4081 ggcatacctc ttgcttaatc tattttaaaa ttgggaaatt tgaaggggtc cgatcttcac
     4141 aggctacgaa cgagcgaact cccctgctca tctgtgactg gcaaccgaaa caacaaccct
     4201 cctctatcag atcggacccc ttcaaatttt ctggatcgtc acgggatgtc agccggtttc
     4261 ttttctgttc cagatttggg aggaagtgtg gaaaagaaaa cgattttggt cgtagacaat
     4321 ggggagacaa tccgcgaaac cctggtaaag attctcgaac gggaaggata ccgtgttctg
     4381 gcggcggagg acggtcaggc ggccctggac gtcttgaagg agaacgaagt caacctgatc
     4441 ctttctgatg tctgtatgcc gcgaatggac ggaaacaagc tgctgaagca ggccaaggcc
     4501 atccatcccg atgtggaagt cattctgatg accggccatg gaaagaccga aatggggctg
     4561 gaagctcttc gagccggggc cttcgatttt atccacaagc cttttaccaa actggccttg
     4621 aacaagacca tcaaacaggc tctggaaaag caggccatgg ccgcggagat gcgtttcctg
     4681 aaggaacggg ttcgagaact gctgggggaa atcatggacc tggtcggaga gccggcggtc
     4741 caccggatgg ggtactcccg tagcttacgg gccgattcaa cccactgaaa acccacgggg
     4801 atataggttt agaacagccg aaatcatcgc tcctgagcca aaaaactttc ctttcatagg
     4861 gaaaggggag ggagaggtat aggctgtttc tttatttttt gaaacttagg cttgcagaag
     4921 aagcgcggaa taaggataat aactttttgt tttaaaacat cccatcggag gttatctatg
     4981 gcagaagaga agaagacaat taccagtatg atggaggaga aacgaacctt ccctcccagc
     5041 aaggaattca gctccaaggc ccacatcaaa agcttggaag attacaaaaa gatgtatgag
     5101 aggtccatca aggaccctga cggcttctgg ggtgaacagg ccaaaaccct ggagtggatc
     5161 aagaagtggg acaaggtcct ggactatagc ttcggtgaca acctgtacat caagtggttc
     5221 cagggcggga agatcaacgt aaccgtgaac tgcctggacc ggcacctgaa gacccagcgg
     5281 aagaacaaga tcgccctgat ctgggagggc gaaccggagg gagacgcccg aaaatacact
     5341 taccaacagc tgtatgaaga agtctgcaag tttgccaatg tgctcaagaa gaagggcgtg
     5401 aagaagggag accgggtcac catctatatg cccatgatcc ccgaactgcc cattgctctg
     5461 ttggcctgcg cccgcatcgg cgccatccac agcatcgtct tcggcgggtt cagcgccgat
     5521 tccctgaagg accgcatcct ggatgccaat gccagcgtgc tgatcaccac cgacgccagc
     5581 taccgcagcg gcaaagtcat cgggctgaag accaatgccg accaagccct ggccagctgc
     5641 ccgggggtca agacggtcat cgtttacaac cgcaccaaca ccaaggttga catgaaagca
     5701 ggccgggact tctggtggca tgaagagatg gccgccaagg acatcaagcc ggaatgcccg
     5761 cccgaagtca tggacgcgga ggatcccctc ttcatgctct atacctccgg ctccaccggg
     5821 aaacccaaag gggttctcca cacccaggca ggatacatcc tcttcgtgat gaccagcttc
     5881 aagtggatct tcgactacaa agatgaagat attttctggt gcaccgccga catcggctgg
     5941 gtcaccggcc acagctacat cgtctatggt cccctggccg ccggggctac cagcctcatg
     6001 ttcgaggggg ttcccaacta tcccaagccc gaccgcttct gggcgaccgt ggagaagtac
     6061 aaggtcaaca tcttctacac cgcccccacc gcgcttcggg ccatgatgcg cgacggagat
     6121 cagtggcccc tcggccggaa tctttcctcc cttcgcctcc tcgggaccgt gggggagccc
     6181 atcaaccccg aggcctggat gtggtactac aaggtcatcg gcaaagagaa atgccccatc
     6241 gtcgatacct ggtggcagac ggagaccggc ggaatcctga tcacccccct gcctggcgcc
     6301 tggccgatta aacccggttc ggccaccaaa cccttcccgg gagtagccgc cgcggtcatc
     6361 cgcgaagacg gttccccggc cagccccaac gaaggcggat acctggtgat caaaaagccc
     6421 tggccgggaa tgatgcggac catttacggg cagcatgagc gcttcaagga gacttacttc
     6481 gttcagttcc ccggcctcta tttcaccggc gacggagccc gggtggatga agacggagat
     6541 ttctggctca tgggccgggt ggacgacgtc atcaacgtct ccgggcaccg gatcgggacc
     6601 gccgaggtgg agagcgcgct ggtaagccat ccggccgttg ccgaagccgc ggtcgtggga
     6661 atgcctcatg atattaaagg ccaggggatt tatgcttttg tcacggtgaa agccggccag
     6721 aagaaatcgg atgaactgaa gaagaccctg gtggctcacg tgcgcaagga gatcggcccg
     6781 attgccactc cggacaaact ccaattcgcc gacgggcttc ccaagactcg aagcggaaag
     6841 atcatgcggc gtattctgaa gaaaatcgcc gcaggcgaaa tcagtgaact gggcgatacc
     6901 accaccctgg ccgacccggc cgtggtgcag actctggtat ccgaaagaca atagccggtc
     6961 aaggaacgga agaggaggga gccggttcca aagaggagcc cgctccctcc attttgccaa
     7021 ggaggctttt tctcatgtcg gatcaaaaaa gcgctgctcc gccggaagcc ggccaaaatc
     7081 gggggtggat tgttaccttt tcggggacgg ggattaacct ggccctagga gtcctctacg
     7141 cctggtcggt catctccaag cagattacca aagaatgggg atggaacgag acccaggcgg
     7201 cacttcccta ttcggtggcc atcgctgttt ttgccttcat gatggtcccg gccggacgga
     7261 tgcaggacaa actgggcccg cgcctggtgg ccactctggg aggtatcttc tgcggaatcg
     7321 gattcatcct cgccagccta gggcagtcgc tgacgggttt ggttttaggg tttggaatcc
     7381 tggccggggc gggaattgga ttcggctacg cttccgccac ccccccggcg gtaaaatggt
     7441 ttcccccggc gcggacgggg ctgatcgccg gcctggtggt ggcgggtttt ggcctggcct
     7501 cggtttatat cgcccccctg gccaattacc tgctttcagc ttttgggatt caaagcacct
     7561 ttctgattct gggaattgcc tttcttttgg cggtggtgct cctttcccag cttttgaaaa
     7621 accctccagt tggatacaaa ccggccggca accccgcacc ggcaaaggcc ggctctgctc
     7681 cgaaagctac ggctgtggcg gccgattacg aatggacgga gatggtccga accccccagt
     7741 tctacctcct gtggatcatg tttgtctttg gcgcaggagc gggattgatg atcatcggga
     7801 aactggccaa gatcgtggac ctgcaagccg ggatcaaggc cgggtttatc ttcgtggcca
     7861 tgctggccat cgggaacgcg gccggcagaa ttgttgcggg agttctctcc gacaagatcg
     7921 gccgaacctg gaccatgttt gccgtattcg ttttccaggc cgtccttatg ttccttttgc
     7981 gtggactgga cacctatggc accctcttct tggcttcggt gctcatcggc tttaactatg
     8041 gggccaacct ctccgtcttc ccctcggcga ccaaagatta ctttggcctg aaaaatttcg
     8101 gggtcaacta cgggtttgtc ttcaccgcct ggggagtagg gggcattctg ggccctatgc
     8161 tgagcgggca gatcttcgat gccgagaaga attttaactc tgcctattta attgccgccg
     8221 tctgtctgct gatcgctgca ggtttgactt tcgttacccg ggcgcccaag gccaaagccc
     8281 gataagggct cgccatcggg caacctgggg aatggaggaa ggctgggcaa gtccgaggcg
     8341 ggaacctctt tggatgatag gccgcggtcg ggagatgcag aataaaaaac cccttctcga
     8401 gttccttcga gaaggggttt tttaatggaa cccgggaaga tggttacttc gccgattggg
     8461 cggcagccac caactcaccg gcaaaagccg ctttttcgac gcgcttggga accattgccc
     8521 gcttcttcag gtcgaccagc cagaagcaga ggtaagagat ggcgaccatg gacacagagg
     8581 ccgctatcac acccaggatg atcgaataaa atttcgcctg tttggccacg atggggtaac
     8641 cgaggatttc gccggcaaat gcatgggagg ccaacccgat caggattatg gagaccgtta
     8701 tcgctatcgt tgattttttc attgtcagtc cctccttcat ctgtgctccc tctctctatc
     8761 ctctggcctc ttgaagagca agcgccatgc caggaatcca gagccccccc agagtacagg
     8821 ataaattcaa ttaactagtt gatttaattt aaatttctaa aattacaccc ttcccatttc
     8881 aaaaaaatcc agcaggcgga ccatggcaaa atgtcaaatg aatgaatatt cattcgggac
     8941 aatatttcat tcttgacaaa aagaacttta aaccggaatt tatgttgaca taatgacaaa
     9001 taatgttttc tagctggaac acactagttt ctttatgctt tttaaaataa tcccctcatt
     9061 cgcagaggaa attaaaatag gtcaaagaaa ttgagatttt ttcccccctt tgatatatta
     9121 aatcaagggc cagaccggtg aaatccttca aagactggaa aatccgctcc aaattgaccg
     9181 cttttactct gttgctcgcc ctcctgccca tgggggttgc ggccgtagtg tctttacaga
     9241 aattcaccga agatttgaag caggcctacg agtcggacct cgatcacatc gtcaccaaca
     9301 tccgtgccat gtgcagggcg cagcaggaac tcctccagaa caaacttacc tcggacctgc
     9361 agatggctca ctgggtcttc tttcggaaca aacgtaagat tgacatctcc cgagaacaga
     9421 tcgtggaatt tagggcagaa gatcaattta ccaaggaggt catccgggtc cgggtacctc
     9481 tttggtccat tgacgaccag gagatgaccc gggattacgt ccttgtggac caagttcaac
     9541 agctggtcgg ggggacctgc accgttttcc agcgaatccc gggtgaccga ctcatgcgga
     9601 tttcgaccaa cgtccttcgg tccgacggga gccgggcggt gggaacctac ctgccggccg
     9661 ccagcgaggt cagccggacc atccttcggg gagaaacgta ccgggggcgg gccttcgtgg
     9721 tgaatgactg gtacattacc gcctacgagc cgattttcga tgatagaaag gaagtcatcg
     9781 gggctcttta cgtgggagtc ccggagcaga gagccgtggc tttgaagacg gccatcaagt
     9841 ccaccaagat cggcaagacc ggctatgcct tcatcatgga tagttcgggg aggctggtcg
     9901 tccacccggc aaaagagggg gaaacaattc tggaggccaa ggattctgcg ggttttgagt
     9961 acatcaagga gatgattcgc ttggctcccc aactggggga gacagaggtg gggaccctcc
    10021 gttatccttg ggccaatgtg gaattagggg agtccacccc caggatgaaa attaacaaat
    10081 acgtttattt ccgggactgg gactggatca tcgcggccgg cagctacgag gaggagattt
    10141 ttgaaggggt ggcccggacc aagtttttca tcaccctggt ggctctgggc acggtggccc
    10201 tggcaatcct gcttgcagtc atgctctccc gcgtgttaac caaaccgctt caggagctga
    10261 ccgaagtgac cgcgcggatg gccggggggg acctgaccca gaaagtcaag ctcagcgggg
    10321 gcgatgaaat cgggaccctg tcccattcct tcaaccgcat ggccgatcag gtccagaatt
    10381 atacccgcaa cctggaggag atcgtccaga cgcgcacccg ggaaatccag gaaaaggagg
    10441 aagaataccg gaatctttct cgcctgctca acagcgtact ggagagctcc acggaatatt
    10501 cgatcatggc cacagaccca aacggagtga tcctgcagta caacagtggc tccgtgaaac
    10561 tcttcgggtg gagcaaggaa gaagtgatcg gcaagatgcg catcagccgg accttctgga
    10621 aagacgaccg gtcgcgaggg ttgatacggg tcatctcccg caaggtggaa gaggagggaa
    10681 tgaccgagta ccaggtggaa cgggtgcgga aggacgggtc cctttttacc gcccacgcca
    10741 tcgtaacctc gctcaaggac ccttcgggga agaccctggg ctttgtggag attgcccggg
    10801 acatcaccga aaaactggcc ctggaaaaag agctgtggaa gaccaaggac caggtggaga
    10861 atatcgtcca aagctcggtg gatgccatcg tgaccacgga ccccaagggg aagatcactt
    10921 ttgtcaaccg ggcctttgaa gagatggtgg ggaggtccgg ggaggaaatc atagggcttc
    10981 ccatctacca gttctaccag gatggaatta ccgaggcccg caagatcatg tccatcctgc
    11041 gggaaaaggg aagtctgaag aaccacgaga ccaccgctct caaggtgggc ggcacggtcc
    11101 ccattttgac atccgcctcc ctgctgcgcg acgaaaaggg ccagatcatc ggaactctgg
    11161 gagtcttcaa agacctgacc gagaaaaaga aactggagga agagctgcaa agaacccagg
    11221 cccacctcat ccaggtggga aagatgcgcg ccctggggga cctcgtggcc ggggttgctc
    11281 atgaactaaa taatcccctg atggcggcgg atacctttct ccatgtcatc cgggagagac
    11341 tcagcccgga ggacgaaaac ctgaagcgcc tcgaactgat tcaaaagtgc cacgagcgga
    11401 tcgccaagat tatcaaccac ctccgggagt tctcccggca gtccgagttc gacttccggc
    11461 ccttgaatgt gaccgagccg atcgaaaatg ccctgatgat caccggacag cagatgatca
    11521 accacggcat ccggatcgtc aaggaatttc agcccgacct ccccaagatc cggggagacg
    11581 ccaatcagct tgagcaggtg ttcctcaatc tcatctccaa cgcccgggat gccatggaaa
    11641 aggcagaacg gaagaaagaa ctcaccatcg ccgcttctct ccttcgccat aacgggtgga
    11701 atgatgtgga agtgatcgtg aaggacaccg ggaacggcat tccccccgaa aacctggaca
    11761 aagtttttga accctttttc agcaccaagg aggtgggccg gggaacgggc ctgggtcttt
    11821 ccatctgtta cgggatcatc gaagcccacg gggggaggat cgaggtggag agccgggtga
    11881 aagaggggac gactttccgg gtgtttctgc ccgtcttgtc gtcccatctg ggaacggctt
    11941 tagaatggac cgtggaaaca taggaggtgg acggtctggg tcgaagttcg aatcaacctt
    12001 tcgaggtgaa tgaaaaggca tgggtaaaaa aatattggtt gtcgacgatg acgagctggt
    12061 tctgatcgcc attcaggaac tactcacccc tctcgggttt tccgtgaccg cccagtccgg
    12121 cgggcaggag gcattggaga gtgtttcccg gggccgattt gacctggtga tattggatgt
    12181 cagcatgccc gagataaccg ggtttgaagt ctgccaaaag attcggcaga tggaatccta
    12241 cacggatatt cctatcctca tgctgaccgc ccgaagcggg gaagaggata aacagcgggg
    12301 gatggaggcg ggagccaatc tttacctgcc caaacccatt tctcctaaga ggctgatcgc
    12361 gctggttgag gaagctttaa aataaagggg aaagggcaag aggggaaaag gcgaaagggg
    12421 aagcaggaag tcttttgttc ccctcttgcc cctaaattaa aggaggtttc gttcgatgga
    12481 gcatgtgatg ggaaaggcgg agagcttgct aagggaatgg aagggtgatt cttatacctt
    12541 cgggtttgaa gttctgggaa gaaccgggga tattgtcgcc gcgcatggga agcgagccct
    12601 agtgatcgtg gccgatctgg ggcagggctg gatggcggga accctgcaga cgatcaccga
    12661 ttccctcaaa gccagagggg tggaaacgga aatcatcatt ggggccgggc ctaatgcccc
    12721 gcgggaggac gtgtatcgat tggcccttca tgtgtccagg acgaggccgg atgtcatcct
    12781 ggccgtgggg ggaggcagta ccatcgatgc cgccaaagcg gccggcgttc tggcgaccct
    12841 ttctcccgaa accgtgaaga aggctctcct ggctccggat gccctggcca gcaccatcga
    12901 cccctatttt ggcgtgggca acgtgaccaa ggtcaagggg atagccggga agcctctcct
    12961 gcccctcgtc gccgtacaga cagcggcaag ttcgggggcc cacctgacga agtattccaa
    13021 catcaccgac ctggtgaccg gacagaagaa gctgatcgtg gacgaagcta ttgtacccca
    13081 ggcagcggtc tttgattaca gggtcactct ggggtctcct atggacctga ccttggacgg
    13141 agggctggat gggatcgccc atgcttggga ggtcttcatg ggggccaccg gacaggccta
    13201 ttatgaaaaa atgaaagaag tggccaagac ctgccttgct ctgattgtgt tcggactgcc
    13261 caaagtaaaa acggaccctg gggatcgcaa cgcccggcac agtctgggtc tgggcaccga
    13321 cctgggggga tactcgatca tgatcggcgg gaccagtggt ccccatctgg ggagcttctc
    13381 cctgatcgat gtcctgacgc atggacgggc ctgcgcgatc ctcaacccct attacaccgt
    13441 gctttttttc tccaaaatcc aagatcagct gggagtcttc gcagagattc tcaaagaggc
    13501 tgggttcatc aaggccaaga cggaaggcaa gaaaggaaga gccctggcca tgatggtggc
    13561 caggggaatg atccagtttt ccaaagccct cggcttcccg gcaaccctgg cggatgcagg
    13621 agtttccaaa gctcacatta agcggatgat caatgcggcc aaagaccccc agctcaagat
    13681 gaagctgcag aacatgccta cccccatgga agctgagcgt ggggacgtgg acaagttaat
    13741 gagacccacc ctggatgccg ctttctctgg ggacctggac ctcatcccgg cagtggccgt
    13801 gtagttcaat agcggattac accctgagcc aggtaaggtt cagggtgccc tgcactcgac
    13861 gtagtgcagg gcggaattcg gaatggggaa tgcggaacgt aggggcaggg cgcgcgcccc
    13921 cgaaatcccc ggggttctct attccgcgat tcgcgttcga aggagagctt ttcgatatga
    13981 gacctgactt gctagaaacc ttaaagaggg tgaaggccaa aggctggaag aaggtgcgcg
    14041 tagaattcac cggcggccct ctggtcatct cagtgccgcc ggactgcgtt gaactgacca
    14101 tgaaaagggc tgacgttctg aaaaacccag cgagagacat tcaaaaagcc cttgaccagc
    14161 cctgcgcagg ccctacgttg gaagagatca tcgggagaaa ggggaaggat gcggaccagt
    14221 tgaaagtggc cattactata tccgacatca ctcggccggt gccctaccaa ggaaagagcg
    14281 gaattcttcc acccctgttg aagaagctgc actccctggg aatccgccgg gaaaacatcc
    14341 tcatcatcgt gggaacggga acccaccggg gcagcacgga gaaagaaaaa gcggagatgc
    14401 tcggcaagga aatcgtatct gcctatcaga ttcttgatca tgattgtgaa gatgaatcca
    14461 tcctggtctc ggtgggccag acccccacgg ggacccaagt ccacctgaac cggctcttcc
    14521 accaggccga tgtgaagatc gccaccggcc tgaccgagag ccattttatg gccggagcct
    14581 cgggcggacg aaaggccgtt tgcccggggt tggtggacct ccgcaccatc cagaagttcc
    14641 acagcccgga gttccttgaa tcgccctgcg ccaccaacct ggtcttcgaa ggaaacccct
    14701 gccatgaaga ggccctagcc gtggcaaaag cggtgggtat tgatttcacc atcaccgtaa
    14761 ccctggacaa agacatgcgc atgaccggag tctttgccgg agacctggag aaggtactgg
    14821 aatttgccgt ggagaagatc aaaacctacg tatccattcc cgtggaggag gagttcgaca
    14881 ttgtcttaac ccacggggga tatgtaggcc ggaaccatta ccagaccgcg aaagcggcgg
    14941 tgggagccat cccggcggtg aaggagaagg gggttatcat catcgccgcc gacaaccgcg
    15001 acccggaacc catcggcggg cccgagtaca agaccttgac ccatcttctg aaactccagg
    15061 gtcccgaccg atacgtggag gtcctcaaat ccccctcctg gaagttcacc aaggaccagt
    15121 gggagccaga ggtatggggg aaggtcttga ggaaggtggg agacgagggg ttgatatatt
    15181 gcacccccca cattcctgag gaaacttttt ctatgttacc gggggtgagc ggttatgaat
    15241 tcctcactcc tgcaggaaag agaaaaagcc gggcaaagaa agcggaagag atggtgcaga
    15301 acacgatttt cttttattac cagacctatc gagaaaaatc catctccccc cgagtggcct
    15361 tcatccgcga aggcccgtat gcggtccccg taggaagcta agaagctcta ggtcccacta
    15421 aaggccgttt ttcctttgtt gcccttgtcc ccagtccaca actcaggcat tctggaaata
    15481 tcaatccgcc acgagtggga cccccggcag agctggaggt ataccttaag gccattatct
    15541 taaatctcga atggaagcaa ggcttgaaaa tgtctctttc agaccggtcg aaagtatcct
    15601 tcgatcggtc atacaggtca atatttttgc acccagcccc acccagtgct tggcggattc
    15661 cctgatctcc gccggggacg cctcgaataa atgcatatga acggccacgt tgttccccag
    15721 agcggtggtg attcctcttt cgattttccg ttttacctcg gggtgatcgg ttcggccggc
    15781 gaaccccatt tcctgggaca ggtcgaaggc tccaaaagca accgcatcga tcccgggagt
    15841 cttcacgatc tcttccaaat gctgaaatcc ctctagggtt tcgataatca gccaaaccat
    15901 cacattttgg ttcgaccact gcacatgttt ttcccaagga tgaattccat gggccgtcgc
    15961 ccgtacccga gggcaggccc ctcttgttcc caccggagca tatcgagctg catgggcgat
    16021 ccttccggcc tgctccgctg tggaaacccc cggcagaacg attcccgtcg cccctgcgtc
    16081 caggacctta tggatgttcg tttccgaatg gtccggaagc cggaccatgg gggttgcccc
    16141 tccgatttgg actcctcgaa tcaggtgaac cagttgatcc atgccgaacg atccgtgctc
    16201 catgtccagg atgaggaaat cgaacccgca ggctgcagcg atttcggcat tctccgggct
    16261 tgctgtctga atgaacgttc ccaacgcaaa ccctgatttt tggatcgcct ctttaacccg
    16321 gtttcggtaa atcataagtc tctcggtccc cccattgctc cgacatcagc gaaaggaact
    16381 tctctctttc aaagaatact ccccatttcg gaccgtttac ccatgaaact tacaatcgtt
    16441 ggtcgtgacc ggccccctga aaccgcatca attctctccc gatcaccaga agctgaatct
    16501 ccgaagcccc ctcgtagatc ctcggggccc gcacggcgcg gtaaagattc tcgattcggg
    16561 acttccggtg aagcccgatg cccccgtgaa tctgcaggga ctggtccaca accttttgag
    16621 ccgtttccgt ggcaaagtat ttggcagccg atgcttccag ggttgttttt tctcggaggt
    16681 tgtcggccag ccaagccgcc cggtatacca ggagggaggc agcatccaaa gagactttca
    16741 tctccgccag cttgaattga gtcacctgaa aatcagacag gacccgaccg aacatcgggc
    16801 gtttctgggc gtattccagc gccagggaca aagccctcca tcccataccc agggcggcgg
    16861 ctcctacggt cggacggtag atatccaggt ttcccagagc gatgcgcatg ccttgccctt
    16921 cttctcccag gcggttttcc ccgggaactc tcgtgtcctg caacaaaacc tctccgatgt
    16981 tgcactcgaa ggtcaaacgc tcagtcctca ccccatagga agactgctcc cggtccacga
    17041 tgaacgccga gatccccttc ccccctgctc ccgggtcggt cttggcaaac acgaccgaga
    17101 tatccgtgtg cccgggcctg gaaacgtacc acttgcggcc gttcagaaga taaaaatccc
    17161 cctcccgcct ggccgtggcc tggatacctg ccacgtcgga acccgagctt ggctcggtaa
    17221 gacagaagtt cgccatccgg gtcccgtcca ccaggggagg caaatacttt ttcttctggt
    17281 tttcgttccc gaagagcacg atggggttgc tgcccagtcc ctgcatgatg aaggcttcat
    17341 cggcgaatgt ggatatcttg gccagttcct cacggacgat gcacaggttg accgaagaaa
    17401 tccccttgcc cccataggct tcggggacca cataggcgta gagaccggac ttcctgatgc
    17461 gatttattcc ttcggcatgg taatcataag cggcatccaa ctcctcggcg aatggctcga
    17521 tttccttttc cgcaaagtcc ctcgccctat ggcggagctg gacctgatcg gtagtgaaag
    17581 tgaaatccat ggttcctcct ttgcagaaaa cgcttgagat tggaaaaggg tttgaaccgg
    17641 aagagaaaga gggtctaccg agattatttc cttttattat tttgtccgtt cctttgtcaa
    17701 gtcctttgtc ggatgatgga atttggaatc catcattttg cacttcgccc ccggcaatcc
    17761 ccaaatggaa agcccggaaa gcgaaagaag gaatatcact acaatggggc caatgattct
    17821 gctatgatgg gcacggtgag gagcagagaa taagaaagcc tgaaagggca aggagggttg
    17881 agcaatggaa tggaggcgtt tccggctggt gggaaaggga agtcgggctt ttttcttgac
    17941 agtggctacc cttttcttag tctgcgggac ttcggcccct aggtctgttt ttgctcaaga
    18001 gaaatacccg gtgaagccta ttaattttct catcgggttt ccagcggggg ggacgacgga
    18061 tgtctgcgcc cgccctctgg taaatgccgc gagcaagatt ctggggcagc ccatcgtcgt
    18121 cgtaaacaag cccggcgggg cttctgcggt cgcggtggcc accctcaaga acgaaaagcc
    18181 ggatggatac accatcggaa ttctgggcag cggggcggtc ctgagccagc acatgcgtaa
    18241 agtcccctac gacacggcaa aggacttcac ccctatcatg cagtacgcag tatacctcta
    18301 cgggctggtg gtgcagtccg aatccccatg gaagacgttt aacgaattca tcgagtatgc
    18361 caggaacaac ccggggaaaa ttcgatacag tacggccggt cccggaagcc cccaacacct
    18421 ggtcatggaa cggttggccc tgaaggagaa gatcaaatgg acccacatcc ccttcccggg
    18481 gggagggccg gcggtaaccg ccttgctggg tggccatgtc gaagccactt cccagaccac
    18541 cgaatggaag gcccatgtgg aatccgggcg cttgagactc ctggccgtgt acggagaaaa
    18601 acggatgatc gattttccca atgtgcccac ccttcttgag ctgggctacg acatttccgc
    18661 cgccagtctc atctccatcg cggggcccaa ggggatttcc ccccagatcg tggaaattct
    18721 tcacggggcc ttcaagaagg ccatggaaga cccggacttt gtcaaggcca gtaggcaatt
    18781 ggaccagccc acggtttacc gcagccccga ggacctgggc aagcacctgg tcaaaatgaa
    18841 tgaggaagtg ggcggtttag tccggagctt ggggctgcgg gaagaataag gggcggacgc
    18901 gaaatcttac agcactcgcc ccctttccct agaaaggagg aagttccgct gaattgtatg
    18961 aggaccggta aggaagcggt catggtcctt ccccgcggga ggattttttg atcgtccctg
    19021 tgagtctgag cccgcctacg aagtaggcaa cggaggaccc atccatggag aagaaaaaag
    19081 aaggcaagaa agagggtaca ggcaaaaaag ggatcagccg tcgtgcgttc ctccaattcg
    19141 taggggccgg tgccgtggtt tcggcaagcg cgggaaaggg agtagccgcg acccctccgg
    19201 agaccgccga gcctagcgaa aaaatccggg taagcctttt cgttaatggg cggcgccggc
    19261 gcctgctggt cgagccccgc tggtctcttc tctatgtcct acgcgaaaag atgggtttga
    19321 ccggaaccaa agtgggatgc gaacggggag agtgcggcgc atgcacggtg ctcatcaacg
    19381 gccacccgcg ttatgcttgc ctcactctta cggtggaggt tgagggggaa gagatcacga
    19441 cgctggaagg tttaatggag ggagaggaat tgggcgctgt gcagaaggcc ttcgtcgagc
    19501 atgatgcttt ccagtgcggg tactgcaccc ccgggcagat catggccgcc gaagggttgc
    19561 tgcgatccaa tcccgacccg acgctggaag aggtccgcct ggggatgagt ggcaacctct
    19621 gccgatgcgg ggtttatccg aatattttcc gggccgttcg gcgggccgcc gagctcaaaa
    19681 gaaatatgag aggtggacga tgagtgccgc agaataccgt gatgagtcag gccagtcctt
    19741 gcggccctgg aaggatacga cgatcatcgg gaagcccatg ccgcgggtcg atggctatga
    19801 gcgggtaagc ggcgcggcgg tcttcccgtc ggatacctcc ctgccgggta tgctctacgg
    19861 ggccattctg cgctgccccc atgcccatgc ccatgtgaag agcatcgaca cccgcaaagc
    19921 ggagaagatg cccggcgtcc gggtcgtgat caccgggaag acgcccgggt gcggagttga
    19981 ctggacatac acaactccga cggcgatggt caagaccaag ctctttgatc cccacctccg
    20041 ccatgagggg gaggaggtgg ccgcagtggc cgccgaaact ccctaccagg cctgcgacgc
    20101 catcaaagcg atcaaggtgg aatacgaggt tctccccttc gtggtggacc ccaagaaagc
    20161 cctggaaggg caggctcctg ccctctacga agggggcaac cgggtcggta agacccagac
    20221 ctatgaacgg ggggacgtga ccagaggctt tgccgaggcc gatgtggtac tggaagaaac
    20281 gtaccatacg gcctgcgagc tccatactcc cattgaactc cacggctgcg tggcgaagtg
    20341 ggatggcccc cgtttaacgg tttgggagcc gacccagggg gttttcgctg tccaggccgg
    20401 aattgcccag gtattgggtc ttcccctggc gaatgtgcgg gtcatcggcc actatgtggg
    20461 cgggggcttc ggcagcaggc tcacggcggg caagtacgac atcgtcgccg ccctgctggc
    20521 caggaagacc ggccgcccgg tcaagctctt cctgacccgg gaggagacct acctctgcgt
    20581 aggcaaccgc ccccctgatg agatgagact caaagcggga gtaaagaagg atggcaccct
    20641 cacggctctt gaatacagcg ccctgggtac ggggggcgcc tatcccggtt cggggaccag
    20701 caatgtggac tggctggtcc gggaccttta cacctgcccg aacgtgcgca ccgataccac
    20761 ggatgtgtac attaatgctg gtccggcgag gccgttccgg gcccccggcc acccccaatg
    20821 cgcctgggca ttggagcaga tgatggacgc cctggctgag gcgattggaa tggaaccggt
    20881 cgagcttcgc ttgaaaaaca tcccttctca tagccaggcg agggggaata ttccctatac
    20941 gacgaccggc ctcaaacagt gtctggaaga aggggcgcgc gccttcggct ggaaagaagc
    21001 ccgtcagaag gcggctgagt ccagaaggaa tgcgggccat atccgccggg gagttggaat
    21061 ggccggaggc ctctgggtgg tcggcggcgg gcggcccccc gtaaccgtgg tagctaaatt
    21121 atttgccgat ggcagcgtta acctgaatat gggcgcgagc gatatcggca ccggcacgaa
    21181 aaccgtcatg gccatggtag t
//
LOCUS       DASPRR010000359         6386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1049663, whole genome shotgun sequence.
ACCESSION   DASPRR010000359 DASPRR010000000
VERSION     DASPRR010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049663"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..1778
                     /locus_tag="VEL68_13525"
     CDS             525..1778
                     /locus_tag="VEL68_13525"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37033.1"
                     /translation="MKKILLASIFLMAALLIFGPGETGAQVKEISIGAVFPLSGPLAP
                     IGQHIRAGIDIAIEEINQAGGIKSLNGAKLKVVYGDSTGDAKVGVTEMEKLISKDRVS
                     VVTGAYQSGVTLPMTTVSERYGIPFYATGSEDQITERGYKYTFRSNEKTSWRVRDQVK
                     FISETGKKFNDPVKTAGVVWENTAWGQGAHRDWLKYLGNEGIKIVVDESYPNSTTDLT
                     PVVLKLKGAAPDAIFYCSYVSDAILLVKTIAEMEVNLKALVGLSGGTADPNFIPGVGK
                     NSLYSFDCVRWEPDIPRPGVKEVCEKYKKRAGVEMDAEVMKQYTYMYVISEAINKAAS
                     TDPKKVRDALASLRLKGPHPAIKFVEDELYFDETGQVFQGNMVGVQFREVDGKIQRVT
                     IWPDKIARKGIAPVWPMPKWSERKK"
     gene            1839..2720
                     /locus_tag="VEL68_13530"
     CDS             1839..2720
                     /locus_tag="VEL68_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37034.1"
                     /translation="MFIYAQAIVNGILIGSIYGLIAFGLTLIFGIMRVINFAHASLLM
                     LAMFIAFFLSRAGIDPYLSLIIEIPFFFVLGYLIQRGLIERVLKMEAGVREPLSALLL
                     TAGLLVLIENLVLLVTGPDYKTAQTLYSGATFKLGSVIVKTPWFYGFLTSILAYIILT
                     LFFKWTRLGQAIRATGQDRDVARLMGIDHYHIYGVSFGIGMALLGVSGAILIPFYYVH
                     PAVGWVFGVSSFIIVILGGLGSISGAFWGGVIIGLIESLGAQFMNATWTIVVVYGVFL
                     AILFIRPSGLLGQKFEW"
     gene            2739..3776
                     /locus_tag="VEL68_13535"
     CDS             2739..3776
                     /locus_tag="VEL68_13535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37035.1"
                     /translation="MANLLRNLGRAISFQACLGLSLIAFLFLLPVFLGSAFFTQVLIM
                     IVFFAYLTACWNIISGYAGQLSIGNAAFMMIGAYTSTLLFMKLGITPWIGMIAGGLLA
                     AAAALLIGYPTFRLRGAYFSIATIVWSEGLRKIFENTEKIGNLTFGGAEGLTIPLMHN
                     APLQYQFIDKDYYYYIILILMLIIVYLTYRIDRAKIGYYLTAIREDEDAAQALGINVR
                     RFKLKAFMISAFFTALGGTFYAQLMLYIEPHGVGGLSLSIEMLLLGVIGGRGTIMGPI
                     LGAFLLVPVREMARTYIGSTYLGAHFVLYGLIMVIVILFLPHGIEEPIKRGWSRFIQR
                     WRWNVSAEGGR"
     gene            3778..4503
                     /locus_tag="VEL68_13540"
     CDS             3778..4503
                     /locus_tag="VEL68_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37036.1"
                     /translation="MAFLEIRGLVKRFGGLVAINNFGMEIEKGEIIGLIGPNGAGKTT
                     IFNVITGVYPGNGGQVLFKGKNINGIKPHDSCMIGIGRTFQVAKPFGGKSVLYNVMVG
                     SFARTQDRRQAESKALQVLEMLGLLPKKDQLGKSLTIADRKRLEVAKALATDPELLLL
                     DEVMAGLNPKETEEAIGLVKNVARGGITILIIEHVMRVIMSLSNRVAVLHHGEKISEG
                     TPLQVSQDEKVIKAYLGEEYVLS"
     gene            4490..5200
                     /locus_tag="VEL68_13545"
     CDS             4490..5200
                     /locus_tag="VEL68_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37037.1"
                     /translation="MSFLEVKGIDVYYGDVPAVRQIHLWIGKKELISLIGGNGSGKTT
                     TINTISGLLQPHQGEIWFEDERIDLLPAHRIVDRGIIQVPEGRRLFPLMTVLENLKIG
                     AYAKRVHKELRETLQFVFGLLPVLEERKDQTAITLSGGEQQMLAIGRGLMSKPRLLML
                     DEPSLGLAPLIVKKVFETVKKVNNQGTTILLVEQDVKHSLELAHRGYVLENGRINLEG
                     TGADLLGNSQVKNAYLGI"
     gene            5345..6337
                     /locus_tag="VEL68_13550"
     CDS             5345..6337
                     /locus_tag="VEL68_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXZ37038.1"
                     /translation="MQVDGERLKMIYVLMKRCRAFEERAIVELRKGMPGFIHSSIGQE
                     AIPCAITPFLRNEDYVITNHRGHSDIIARGASFKKTFAELYAKETGYCKGLSGSMHIA
                     AFDLNIIGAVAIVGSGIPLASGVALACKAKNLERIAVCYFGDGATCTGSFHEGIGFAA
                     TFDLPVLFVCQNNQYEEGTHWTYWGGRFKSLADRSSGYGIPGFSVDGNDAVAVAEAAA
                     EAVEKVRKGGGPILLEGKTYRIHGHHMGDPGTTYRTKEEVEEWKGKDPIRRLYSLLFQ
                     KGFITAEENSQIEVQIEQELDEAVQFALESPEIDPHTTFRDLVYFERPVGLNKI"
BASE COUNT         1492 a         1562 c         1830 g         1502 t
ORIGIN      
        1 gggctttgag caaagaagac caggaggcgt ttgaccaccc ttttgaccgg gccaaattgc
       61 ccaccggttc ctcgtccccc aattccctcc acggtctcgt cgtaaccgcc ctttcaggcc
      121 tggattagct ttcgtgggag tcaaagaaag ctagtggcaa gagagttaat gatatttgac
      181 tatttggtcc ttatacgtta aactctcccc tactgtagtc aaaaaaaatt ggggggtata
      241 ttttaaaaaa ccttttcggg tgatcagtta ggtcatgtcg gttgggttgt tccttttcgg
      301 aggagggacg gcacctagcc ggagcagtaa aacctacatt ttaaaaccaa tcccgcagac
      361 ggttcacacc ggcttttttg atgctaccat tctacctgtg ttgaccattg attcgggtga
      421 tacggtggtt tccctggcca gaggggaggt gggacaaaga aagattcctt aagacgagga
      481 acaatatgag cttggcaata aaaaatttcg taaaggaggg ttacatgaaa aaaatccttt
      541 tggcttcgat tttcttgatg gctgcattat tgatttttgg gcccggagag acaggcgccc
      601 aggtcaaaga aatcagcatc ggggcagttt ttcctctttc cgggcctctg gccccgatcg
      661 ggcagcacat ccgggcgggc atcgacattg caattgaaga gatcaatcag gccgggggca
      721 tcaagtcatt aaacggcgca aagctcaagg tggtctatgg ggacagcacc ggcgacgcca
      781 aggtcggcgt gaccgaaatg gagaaactca tcagcaaaga cagggtctcg gtggtgacgg
      841 gagcctatca aagcggagtc acccttccta tgacgaccgt gtccgagcgt tatgggattc
      901 ctttttatgc caccggatcc gaagaccaga tcacggaaag aggctacaaa tacaccttcc
      961 gttccaatga aaagacctcc tggagggtga gagatcaagt caaattcatc agcgaaacgg
     1021 ggaagaaatt caatgacccg gtaaagaccg ctggggtcgt atgggaaaat acggcctggg
     1081 ggcaaggggc gcacagagac tggttgaagt acctgggaaa cgaagggatt aaaattgtag
     1141 tcgatgagtc ctaccccaat tcaacgaccg atttaacccc cgtggtcctc aaattgaagg
     1201 gcgccgcccc cgacgcaatt ttctactgtt cttatgtatc cgatgcaatt ctgcttgtga
     1261 agaccatcgc cgaaatggag gtgaatctga aagcgctagt cggtttgagt ggagggacag
     1321 ccgacccgaa tttcattcct ggcgttggaa agaattccct ctattctttt gactgcgtgc
     1381 gctgggaacc ggacatcccc cgcccggggg tcaaagaggt ttgtgagaaa tataagaaac
     1441 gggccggggt cgaaatggat gctgaggtca tgaaacagta cacctatatg tatgtgatct
     1501 ccgaggccat taacaaggcg gcctccacgg acccaaagaa ggtccgcgat gccctggcga
     1561 gccttcgcct aaaagggccc caccccgcca ttaaattcgt ggaagacgaa ctctattttg
     1621 atgaaacggg gcaggttttc cagggcaata tggtgggcgt tcagtttcgc gaggtggacg
     1681 gcaagattca gagggtaacg atctggcctg ataaaatcgc ccgcaaggga atcgcccccg
     1741 tctggccgat gccgaagtgg tcggagcgca aaaagtaaga ggctttgtag gggggtacgg
     1801 gctaagtacc cccctgcaaa gaaagacaga gacgtaacat gtttatctac gcgcaggcaa
     1861 ttgtaaatgg aattctgatc gggagtatct acggcctgat tgcctttggt ttgaccctca
     1921 ttttcggaat catgcgggtg atcaattttg cccatgcttc gctgctgatg ttggccatgt
     1981 tcattgcttt ctttctgtcc cgcgcgggga tcgatcccta tctctccctg attatcgaga
     2041 tccctttttt cttcgtcctc ggatacctga tccagcgagg tctgatcgaa cgagtgttaa
     2101 agatggaggc cggagtccgg gaacccctga gtgcccttct tttgaccgcc ggcctgctgg
     2161 tgctgatcga gaatctggtt cttctggtca cgggtcccga ctacaaaaca gcccagaccc
     2221 tctattccgg agcgaccttc aaactgggca gcgtcatcgt caaaactcca tggttttacg
     2281 ggtttctcac ctcaattctg gcttatatca tccttactct ttttttcaaa tggacgcgac
     2341 tcgggcaggc catccgcgcg accggccaag accgggatgt cgccaggctg atggggattg
     2401 accactacca catctatggc gtttccttcg ggatcggaat ggctcttctg ggtgtctcgg
     2461 gggctatttt gatccccttc tattatgttc accccgccgt cggctgggtt ttcggcgtaa
     2521 gttcctttat tatcgtgatc ctgggaggac tggggagcat ctcgggagcc ttctggggag
     2581 gggtgatcat tggattgatt gaatctctcg gtgcgcaatt catgaatgcc acctggacca
     2641 tcgttgtcgt gtacggggta ttcctggcaa tccttttcat cagaccttcg ggactgctgg
     2701 gacagaaatt cgagtggtaa agaaggctga gggatgacat ggccaacctt ctgaggaatc
     2761 tgggacgggc gatctctttt caggcttgtc ttgggttgag cttgatcgct tttctttttc
     2821 ttttgcctgt gtttctgggg agcgcctttt ttacccaggt gctgatcatg atcgtctttt
     2881 tcgcctacct gacggcctgc tggaacatca tttccgggta tgccggccaa ctctcgatcg
     2941 ggaacgccgc ctttatgatg attggggcct atacttccac tttgctgttt atgaaactgg
     3001 ggattacgcc ctggattgga atgatcgccg gggggctcct ggccgctgcg gcagccctcc
     3061 ttatcgggta cccgacgttc aggctccggg gggcctattt ttccatcgcc acgatcgttt
     3121 ggtcggaggg actgagaaag atatttgaaa atacggaaaa aatcggaaat ttgaccttcg
     3181 ggggagcgga gggcctcacg atccccctta tgcataacgc cccgctccaa tatcaattta
     3241 tcgataaaga ttattactat tatattatcc tcatcctcat gctgatcatt gtgtacctaa
     3301 cttaccgcat cgacagggcg aagatcggtt actatctgac cgccataagg gaagatgagg
     3361 acgcggctca ggctctgggc atcaacgtcc ggcggtttaa gctgaaagcg ttcatgatca
     3421 gcgccttttt taccgccctg ggcgggacct tttacgccca attaatgttg tacattgaac
     3481 cccacggcgt ggggggattg tccctttcga ttgagatgct cctccttggg gtgatcggcg
     3541 ggagggggac gatcatgggg cccattctgg gggctttttt gctcgtgccc gttcgggaga
     3601 tggcccgcac ttatatcggg tccacctatt tgggcgcaca ttttgtgctc tatggcctga
     3661 ttatggtaat cgtcatcctg ttcctgcctc atggtatcga ggaaccgatc aagagaggat
     3721 ggtctcgttt catccagaga tggcgatgga atgttagcgc ggagggagga agataaaatg
     3781 gccttccttg agattagggg tttagtcaaa cggttcggcg ggctggtggc catcaacaat
     3841 ttcggtatgg agatcgaaaa aggcgaaatc atcggcctca ttggccccaa tggtgccggc
     3901 aagacaacca tttttaacgt gatcaccggg gtttaccctg gcaacggcgg gcaggtcctc
     3961 tttaaaggga aaaatatcaa tggaatcaag ccgcacgatt cctgcatgat cggcattgga
     4021 aggacctttc aggtggccaa acccttcggg gggaaatccg ttctctacaa tgtcatggtg
     4081 ggcagctttg ccaggaccca ggaccggcgg caggctgaat ccaaggctct gcaggttctg
     4141 gaaatgttgg gattgctccc gaaaaaggac cagttgggca aaagtcttac gatcgcggac
     4201 cggaagcgcc tggaagtggc caaggctttg gcaacggacc cggagttgct cctcctcgac
     4261 gaagtgatgg ccggcctcaa ccccaaagaa acagaggagg ccataggcct cgtgaagaat
     4321 gtggcccggg gggggattac cattttgatt atcgagcacg taatgcgggt catcatgagc
     4381 ctttcgaacc gggtcgcggt tttgcatcat ggtgagaaga tctccgaagg gaccccgctc
     4441 caggtttccc aggatgagaa ggtcattaaa gcctacctag gagaggaata tgtcctttct
     4501 tgaagtaaag ggaattgatg tatattacgg ggatgtgccc gcggtcaggc aaattcacct
     4561 gtggattggg aagaaagaac tgatcagcct gatcgggggt aatggatcag gcaagacgac
     4621 gaccattaac acgatctccg gcctgctgca accccaccag ggggaaatat ggttcgaaga
     4681 cgaaaggatc gatctgctcc cggcccaccg cattgtggat cgagggatca tccaggttcc
     4741 cgaagggagg cgtttgttcc cgctgatgac ggtcctggag aacctgaaga tcggggcgta
     4801 tgccaaacga gttcataagg agttgcggga aacccttcaa ttcgtctttg gcctgcttcc
     4861 cgtattggag gagcgaaagg accagacggc gatcacgcta agcggtggag aacagcaaat
     4921 gctggccatc ggccgaggat tgatgagcaa gccgcggcta ttgatgctgg atgagccctc
     4981 tcttggtctt gcgccgctaa tcgtaaaaaa agtttttgag accgtaaaaa aagttaataa
     5041 ccaaggaact actatcctcc tggttgagca ggatgtgaaa cactctttgg aactcgcgca
     5101 ccgcggctat gttctggaga atgggaggat caaccttgaa gggacgggcg cggacctttt
     5161 gggcaacagc caggtgaaga acgcttacct cggaatataa ccagctcgga ccgcagcctg
     5221 taaatccgcc gcgggaatca acctcaaaaa tcaggggtcc tcacctgggg aagatataag
     5281 ctttggggtg taaatgaggg cgagaccccg gggcggaaga atcttttcat catagggggg
     5341 aagtatgcag gtcgacggcg aaaggttgaa aatgatttac gtattgatga agcgatgccg
     5401 ggcctttgaa gagagggcaa ttgtcgagct tcgcaagggc atgccggggt ttattcactc
     5461 ctccatcggg caagaggcga ttccttgtgc gattacgccc ttcctccgca atgaagatta
     5521 tgtcatcacc aaccaccggg ggcacagcga catcatcgcc cgcggggcta gcttcaagaa
     5581 gaccttcgcc gagctctatg ccaaggagac tggctactgt aagggattgt ctgggtccat
     5641 gcacattgcc gcttttgacc tgaatatcat cggggctgta gctatcgtgg ggagcgggat
     5701 cccccttgct agcggcgtgg ccctggcctg caaggcaaag aacctggaaa gaatcgcagt
     5761 ctgttatttt ggcgacgggg caacctgcac ggggtctttt cacgagggaa ttggatttgc
     5821 ggcgaccttt gatctccccg tgctgttcgt ttgccagaac aaccaatacg aagaggggac
     5881 gcattggacc tattggggcg ggaggtttaa atccctggcc gatcgttcct ccgggtacgg
     5941 gattccaggc ttcagcgtcg atggaaacga tgcggtcgcc gtagccgagg ccgcggcgga
     6001 agctgtggag aaagtccgga aaggaggtgg cccaatcctc ctggaaggga agacctaccg
     6061 tatccacggg caccacatgg gggatcctgg gacaacctac cggacgaagg aagaagtgga
     6121 agaatggaag ggaaaagacc ccattcgccg gctttactcc cttctttttc aaaaaggttt
     6181 catcaccgcc gaggagaatt cgcagatcga ggtccaaata gaacaggaac tcgacgaggc
     6241 ggttcaattc gccctcgaga gccccgaaat cgatccccac accacgtttc gggacctggt
     6301 ctactttgaa cggccggtgg gtctgaacaa aatctgacga ggtcagccgt gcgcgaaaaa
     6361 acttaccggg aagccttgcg ggaagc
//
LOCUS       DASPRR010000360         6860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1534625, whole genome shotgun sequence.
ACCESSION   DASPRR010000360 DASPRR010000000
VERSION     DASPRR010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534625"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VEL68_13555"
     CDS             complement(<1..406)
                     /locus_tag="VEL68_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37039.1"
                     /translation="MVNKRKFVAMGLMVLLGISIAHLAWAAEPIKIALFDPRSGPFKP
                     TGDTYAWALEFLVEEINEGGGLLGRKVELIEEDSQLKPDVAVRKATKNVMDGVKFIAT
                     GTGTHIALALQQVAAKEKVIFFSYGAEGDEVTG"
     gene            complement(681..1445)
                     /locus_tag="VEL68_13560"
     CDS             complement(681..1445)
                     /locus_tag="VEL68_13560"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HXZ37040.1"
                     /translation="MRDRIDLNCDMGESYGAYRLGRDSEVMEYISSANIACGWHAGDP
                     LVMDYTVRLAKEKGVGVGAHPAYPDLMGFGRRRMGLTSDEIETYVIYQIGALYGFTKA
                     HGIPLQHVKARGALGNMAFADLEVAKAFARGVLRFSSEIILVVYTGTVMVKAAKEMGV
                     RFVEEVYADRVYNPDGTLQSRKIPGSVIHDPEKAARQALTMAKEGFVIAHDGTKVKVI
                     PESICVHGDTPTAVSILQRIREGLRGASIAVKPMGE"
     gene            complement(1781..2572)
                     /locus_tag="VEL68_13565"
     CDS             complement(1781..2572)
                     /locus_tag="VEL68_13565"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ37041.1"
                     /translation="MQLQGQVALVTGAGRGIGKAIALRLARAGADIAIADLNHEWAQA
                     TGDEIRGLGRRVFCAHTDISDYDQVQTLVANTVQRLGKIDILVNNAGIAKAQPFLEVT
                     KENWEAHLKVHLFGTFYCSQAAARDMCQRHYGRIIAIASIAGLMGPIDIAPYGAAKAG
                     IIGLIRAMALELGDCGITANAIAPGPVDTELLRTLWSKDMYAERARQVPIRRLGSVEE
                     IAHAALFLASPEASYISGAVLPVDGGAVAAGGYMVEQYRRRLSSS"
     gene            2988..4034
                     /locus_tag="VEL68_13570"
     CDS             2988..4034
                     /locus_tag="VEL68_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ37042.1"
                     /translation="MENLNPIYQELAARLGQAHSKFMPRILAKLANLEQAKILRELPA
                     PAEEIAKKLNLEKSAVDRHIQELMEKGVVFPTKKGPQMARTMEQLHDAALGNPKYDEQ
                     LGAEYFDLFAEFMDHEVVEDMIKTYVTPEGPRFRIIPRWKSIENIPGVLPSEDMREII
                     RKQELLALLQCPCKREHRKRTCGVPLQVCINVGRTAQYNINRGAGKKITAEEALRILE
                     ATDPHPMVHISLNQTEVNQLICNCHKCCCSPMIHMFTQKKHKFTDGIAKSRFEAAVDP
                     QTCKACKVCLTKCQFGAVQMVKDPATGKERSSVDPGKCMGCGSCVVSCRGGARSMKVV
                     RPPEHIPQQIARLY"
     gene            4103..5026
                     /locus_tag="VEL68_13575"
     CDS             4103..5026
                     /locus_tag="VEL68_13575"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HXZ37043.1"
                     /translation="MGVFHCPMGCAYYEDEPCIDCGLCLATTPEEMVLASQRIRDYLR
                     AHSVNREGPSRKIAVCGKGGTGKTTAVALMAGAFQDLGFSVLVIDLDESNPGLRRMLG
                     FGREPKPLLAVMKKPPKGEKPKEAWFTREEMGIQDIPGEFLLGVDSLKFLMAGKIDDP
                     FQGCACSLADLARDLVQKLTLREKEILLIDTEAGVESFGRGIERHVDTVVIMVEPSAE
                     SIALAEKISYLADGIGVKKVRAISNKIPSERTRQRVAEEMERRKIKVLGALGVDDQIS
                     ESSLEGKGPSPHSNAQRFMKEIALRLLGESS"
     gene            5036..5164
                     /locus_tag="VEL68_13580"
     CDS             5036..5164
                     /locus_tag="VEL68_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37044.1"
                     /translation="MGGKTYPDVAARLAGFLIEASYQALPPEGAKPDTRRSGGVFF"
     gene            complement(5191..6684)
                     /locus_tag="VEL68_13585"
     CDS             complement(5191..6684)
                     /locus_tag="VEL68_13585"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ37045.1"
                     /translation="METIYLLLKGFSVALNPENLLLCAVGVITGTLVGALPGIGSAGA
                     CAILLPATFKLGPVGSLIMLAGIFYGTQYGGTITSVLMNVPGESASVVTMFDGNPLAK
                     QGRAGVALGLAAIGSFVGGTLSIVGLMLFGPVLANYALELGPAEHFSLMVLSLSLVCA
                     FSGESIVRGLIATCFGLILALVGQDIMTGQPRLTFDFQQLLDGIEFLPVAVGMFGLSE
                     TIESFEKKSSMEIIRADLGFFKVLPSLRDLGQSFWAMVRGTVIGFLIGIFPGAGPTIS
                     SFISYGVEQRISRNPEQFGKGALAGVAGPETANNAATGGALVPMLSLGLPSSPSTAIM
                     LAALIMFGLKPGPSLFREAPELVWGLIGSMYIGNVLLVIINLACIPLIVLFMDRIRAY
                     LPLAILLLSAFGVYSYRNSVFDIGIMIFFALVGYGMRKVEFPQAPVILGVLLGGAAES
                     SLRQAMVMSDGNPSIFVTRPLSAAFLCLTLASLILPPIFLRKRPKQD"
BASE COUNT         1651 a         1957 c         1861 g         1391 t
ORIGIN      
        1 ttcccgtcac ctcgtctcct tcagcgccgt agctgaagaa aatcactttt tctttggcag
       61 caacctgttg cagggccagg gcgatatgcg ttcccgtccc ggtggcgata aacttgaccc
      121 cgtccatgac gttcttggtt gcttttcgca cggctacatc cggtttcagc tggctgtctt
      181 cctcgatgag ctccaccttc cgcccgagga gtccgccgcc ttcattgatc tcttcgacta
      241 agaattcgag ggcccaggca tacgtatccc cggtcggttt gaatggcccg gatcgaggat
      301 cgaaaagggc gatcttgatc ggctccgcag cccatgccag atgtgctatg gaaattccca
      361 aaagtaccat tagccccatt gccacaaact tccttttgtt taccatgcat ttcctccttc
      421 cgaatttcgg catttcgaac tgccgtagat aaaggttggt tccttggaaa accgaatcgt
      481 ggaaaagaaa agcagctaaa aaatactctt agagcaatcg gtagtcaagg aaaaattcag
      541 aggcaatttg acaattgcaa aatgatcgat agcgtgccat catcataact gccatttttc
      601 cccgaaatat tgcctaaatg atttggatat cgacctattc cccacaaata gggtcaatta
      661 tttcccatgc ttttttgtct tcattcaccc ataggtttaa ccgcgatcga tgcccccctg
      721 agtccctctc ttattctctg gagaatcgaa acggcagtcg gagtgtcccc gtgaacgcat
      781 attgactcgg gtattacctt gactttagtt ccgtcatggg caatcacaaa accttctttc
      841 gccattgtta gagcttgccg ggcggctttt tcgggatcat gaatcaccga tccaggtatc
      901 tttcgggatt gcagggtccc gtcgggatta tagacccgat ccgcataaac ctcttctaca
      961 aaccgaaccc ccatctcttt cgcggccttg accatcactg ttcctgtata tacgaccagg
     1021 atgatttcgc tgctgaaacg aagaaccccc cgagcaaaag ccttcgcaac ttccaagtcc
     1081 gcaaaggcca tatttcccaa agctccacgg gccttgacat gctgtaaagg aattccatga
     1141 gctttggtga acccatataa ggctccaatt tgataaatga cgtacgtttc aatttcatcg
     1201 gaagtcagac ccatccgcct tcgcccaaaa cccatgaggt ccggataggc aggatgggcg
     1261 cccactccca cccccttttc cttggccaac cttacggtat agtccataac taacggatct
     1321 ccagcatgcc aaccacaggc aatattcgcc gaagaaatat attccattac ttcagaatca
     1381 cgccccagcc ggtaggcacc atagctttct cccatatcac agtttaggtc aattcgatcc
     1441 cgcatcatct ctccagagaa aaaatattag agacgaaatt tacaatctct gataaacgca
     1501 gtatgcggat gtcgcgccac taccagacac cgccaatttg cccaaaacaa cgtatagcat
     1561 caacatgcca tgttggcaac tacattcgat taccgaggat tacaagttga aggcgaggac
     1621 tatgcccacg gcgagccgtg cccgatagcc gcagcaacgc aggagctaag actgttggcg
     1681 ggggctccac ggggattgga tagtctctgc tgcagccacc cagcaggaag gcacggcggc
     1741 tcttgctgcc caggggtggg tccgctctag cgcctatagg tcatgaggaa gacaaccggc
     1801 ggcgatactg ctcgaccata tagccgccag cagctactgc gccgccatcc acagggagga
     1861 cagcgcctga gatataactc gcctccggcg aagccaggaa cagggctgca tgggcgattt
     1921 cttccacgct cccgagacgg cggatgggca cctgcctcgc gcgctccgcg tacatgtctt
     1981 tggaccaaag cgtgcggagg agttccgtat caactggacc tggcgcaatg gcattcgcag
     2041 tgataccgca gtctcccagt tctagtgcca tggcacggat cagaccgatg atccccgctt
     2101 tcgccgctcc gtagggcgct atgtcaatag gccccatgag cccggcaata gaggcaatgg
     2161 caatgatgcg gccatagtgc cgctggcaca tatcgcgggc cgccgcctgc gaacaataga
     2221 atgtcccaaa gaggtggacc ttgaggtggg cctcccagtt ctctttggtg acctccagaa
     2281 acggttgggc cttggcgatc ccggcattat tcacgaggat atcgatcttg ccgagcctct
     2341 ggacggtgtt ggcaaccaac gtctggactt ggtcgtaatc gctgatatcg gtatgtgcgc
     2401 agaagacgcg gcggccaagc cccctgattt catcccccgt cgcttgagcc cattcatggt
     2461 tgaggtcggc gatggcgatg tctgcccccg cccgcgcaag gcggagagca atcgctttgc
     2521 caataccgcg gcccgcaccc gtcaccagcg cgacttggcc ttggagttgc attggtgtcc
     2581 tctcctctcc atgagtgcat tcatccacag tcctctgggc gacctcacta aaaaaactca
     2641 ttctatcggc tattggttga actatacaga tttggttagg agcggtcaag ctcgactttc
     2701 gtgaaggagt cttcccttct taatgtgcca ttattttcag actgactccc aaattagggg
     2761 gaaaatccaa taaggtatgc agtgcagtag aatgcaccta aataattcaa ttgacataga
     2821 gggaattcgg gtgatagaca aaagacgatg tcgagattga cttggcttga ctctccgagc
     2881 gtttttcagt gtttatgaag acctggagtc ggcgtggaca ggaatgtttt ctatccattt
     2941 cctggaaatt tgagccagag aaaaagaaaa gggggacgta aagggcgatg gaaaatctga
     3001 atcccatcta tcaagagctg gcggcgaggc tggggcaggc ccactcgaaa ttcatgcccc
     3061 gcatcctggc caaactggcc aacctcgaac aggcgaaaat tcttcgagag ctacctgctc
     3121 ctgcggagga aatcgccaaa aaattgaacc tggaaaaatc cgccgtggac cggcacatcc
     3181 aggagctcat ggaaaaaggg gtggtcttcc cgaccaagaa aggcccccag atggcccgga
     3241 ccatggaaca gctccacgat gccgccctgg gcaatccaaa gtacgatgag cagctgggcg
     3301 cggagtattt cgacctcttc gccgaattca tggaccacga agtcgtggag gacatgatca
     3361 aaacctacgt gaccccggaa ggcccccggt tccggattat cccccgctgg aagtccattg
     3421 aaaatattcc cggggtcttg cccagcgaag acatgcgcga gatcatccgg aaacaagaac
     3481 tcctggccct tctccaatgt ccctgcaaaa gggagcatcg gaaaagaacc tgtggagttc
     3541 ccctccaggt gtgcatcaat gtgggccgga cggcccagta caacatcaac cggggggccg
     3601 gcaaaaaaat cactgctgaa gaggccctcc ggattctcga ggcgaccgac ccccacccca
     3661 tggtccatat atccctcaac cagaccgagg tcaatcagct gatctgcaac tgccataaat
     3721 gctgctgttc ccccatgatc cacatgttca cccagaagaa gcacaagttc accgacggga
     3781 ttgccaagag ccgcttcgaa gcggctgtcg atcctcagac gtgcaaggcc tgcaaagttt
     3841 gcctgaccaa gtgccagttc ggcgccgtgc agatggtcaa ggacccggcc accggaaagg
     3901 agcgctcctc ggtggacccg gggaaatgta tggggtgcgg gagctgcgtg gtgtcctgcc
     3961 gcggcggcgc gaggtctatg aaggtcgttc gtccgccgga gcatatcccg caacaaatcg
     4021 cccgcctcta ttaatcggct ccacctgccc actttcttca tggggaggag gggtctcggg
     4081 tggcgaaaca atgggggaac gaatgggagt gtttcattgt cccatgggct gtgcctacta
     4141 cgaagacgag ccctgcatcg attgcggtct ctgcctggcc actaccccgg aagaaatggt
     4201 cctggcttcc caaagaatca gggactatct ccgggcccat tcagtaaacc gagagggccc
     4261 cagccggaaa atcgcggtct gcggcaaagg gggaaccggc aagacgactg ccgtggccct
     4321 gatggcgggg gctttccagg atttgggctt ttccgtcctg gtgatcgacc tggacgaatc
     4381 gaacccggga ctgcgcagga tgctggggtt cggccgggaa cccaaacccc tccttgcagt
     4441 catgaaaaag ccgcccaaag gagaaaagcc caaagaggca tggttcaccc gggaggagat
     4501 ggggattcag gatatacccg gcgaatttct cctgggcgtc gacagcctga aattcctgat
     4561 ggctggaaag atcgacgatc ctttccaggg ctgcgcctgt tccctcgccg acctggcccg
     4621 ggatttggtt cagaagctga ccctgaggga gaaggaaatt cttctcatcg atacggaggc
     4681 tggggtggag agttttggcc gggggatcga aaggcatgtg gacacagttg tcatcatggt
     4741 tgagccctct gccgaatcca ttgccctggc cgaaaaaatc agctacctgg ccgatggcat
     4801 cggagtgaaa aaagtgagag ccatatccaa taaaatcccg tcagaaagaa ccagacaaag
     4861 agtggccgag gagatggaaa ggaggaaaat caaggtcttg ggagctcttg gggtcgatga
     4921 ccagatcagt gaatccagcc tcgaaggaaa gggaccatcg ccccactcaa acgcccagag
     4981 attcatgaag gaaatcgccc tgcgccttct gggcgaatcg tcttaaaggg tctgcatggg
     5041 aggcaaaacc taccctgatg tggccgcccg gctggccggc ttcttgattg aagcctctta
     5101 tcaggccctg cctccggaag gcgccaaacc agacacccgc cgaagcggag gtgtattttt
     5161 ctaggaactg tcccgcatgt cccctttttg ttaatcctgc tttggccgtt tgcgcaggaa
     5221 gatgggtggc aggatcaagg aagccagcgt caagcatagg aaggcggcgg ataaagggcg
     5281 ggtgacgaag atcgacgggt tgccatccga cataaccatg gcctgtcgga ggctggattc
     5341 cgccgccccc cccagcagga cccccaggat gaccggtgcc tgggggaatt cgaccttcct
     5401 catgccgtat cccaccaggg cgaagaaaat catgatgccg atatcgaaaa cggaatttcg
     5461 gtagctgtaa accccgaagg ccgaaaggag caggatcgcc aggggaaggt aggcgcgaat
     5521 ccggtccatg aatagcacaa ttagcggaat gcaggccagg ttgatgatca ccaggagcac
     5581 gtttccgatg tacatgctgc cgatgagtcc ccacaccagc tcgggggctt cccgaaacag
     5641 ggaaggaccc ggtttaaggc cgaacatgat cagtgcggcc agcatgatgg cggtggaggg
     5701 tgagctggga agtcccaggc tcagcatggg caccagggct ccgccggtgg ccgcgttgtt
     5761 ggcggtctcc gggccggcca ctcccgcaag ggctcctttt ccaaactgtt ccgggttgcg
     5821 tgaaatccgc tgttccaccc cgtaggaaat gaaggaagag atcgttggac ccgcccccgg
     5881 aaagattccg atcaggaacc cgataaccgt tcccctcacc atggcccaaa aggattgccc
     5941 caaatcccgc aatgatggga ggaccttgaa gaatcccagg tcggctctga ttatctccat
     6001 ggagctcttc ttctcaaagc tctcgatggt ttcgctcagg ccgaacattc ccacggccac
     6061 gggaagaaac tcgatcccgt cgaggagctg ctggaagtcg aaggtgagcc tcggctggcc
     6121 ggtcatgatg tcctgtccta ccagggccag aatcaggccg aaacaggtgg cgatcaaccc
     6181 ccgtacgatg gactccccgg aaaacgcgca aaccaggctg agggatagga ccatgagaga
     6241 aaagtgctcg gccggcccga gctccagggc ataattggcc aggaccgggc cgaagagcat
     6301 gagccctacg atgctcaggg tcccgcctac gaaagacccg atggccgcca ggcctagggc
     6361 cacacccgcc cggccttgct tggccagggg gtttccgtca aacatggtca ccaccgaagc
     6421 cgactcgcct ggaacattca tcaggaccga agtgatggtc cctccatact gggtcccgta
     6481 aaagattccc gccagcataa tcagggagcc caccggccca agcttgaaag tcgcgggcag
     6541 caggatggcg caggccccgg cagatccgat cccgggcaag gctcccacca gggttccagt
     6601 aatgaccccc accgcgcaca ggagcagatt ctcagggttc agagcgacgg aaaatccctt
     6661 taatagcagg taaatcgtct ccattcgatc cggaatcctt tacagggcca acagaaattc
     6721 aaaaggtccc gcaggcagcg gaacccttag gacgagctgg aagagcaccc aggtggacag
     6781 gctgaaaagg agcccgatcc agatcgatcg ggcccacgaa tatccctgaa ttccctttaa
     6841 ctcgacgaag gaagcaatca
//
LOCUS       DASPRR010000361         4445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2339087, whole genome shotgun sequence.
ACCESSION   DASPRR010000361 DASPRR010000000
VERSION     DASPRR010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339087"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VEL68_13590"
     CDS             <1..333
                     /locus_tag="VEL68_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXZ37046.1"
                     /translation="SAFLQKEDPREACQRIAELGRKVAGVTLGAKGYVALAEGLWIEK
                     PAYPVEAVDTTGCGDVFHAGFIFGVLRGWAYEKCFDFAAWAAAMVSLKMGGRSGIPSL
                     EQVRERGF"
     gene            1006..1956
                     /locus_tag="VEL68_13595"
     CDS             1006..1956
                     /locus_tag="VEL68_13595"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HXZ37047.1"
                     /translation="MGSESRHLSFHGGGGQLFGICLLNTLLTIITLGFYSFWGKTKVR
                     AYLWGKTQLEGDRFVYHGTGRELLNGAFKAMLVFGFPLTLLNTLPKFLEPREILGVVA
                     SILTYAMVILFIPMAMVGARRYRFSRTSWRGIHFSFRGQAREFLRLFLEGWVLTVLTL
                     GLYYPFFDIRRHGFMVSNSYFGSKRFKFDGAGRDLFKAYLLAVLLSLPTLGLYWFWFM
                     AKKQRYFWEHSHLDTLHFRSTVTGGRLLAFYSGNLFLLILTLGLAWSWVVIRKVRFNF
                     AYLTLEGAMDAAKVQQEVQAASATGDALAGFMDASFDLGS"
     gene            2112..3221
                     /locus_tag="VEL68_13600"
     CDS             2112..3221
                     /locus_tag="VEL68_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXZ37048.1"
                     /translation="MSSDLEGYYMDGRTAERHRAGVRLTPMGLQITLENKNSFFWPME
                     EIQQSKNFYGEEQVRLERGGKTPEVLLLPSSIFLKRIKEISPEKKKRFHPPARRKSWV
                     WVVILSAAGVLGMTASLYLWGIPAMTSWMAPYIPVSWEERLGRSVVDTIAPVEKRCED
                     SAQSKKFQEILNALGSSVPHSPYKFRVMVVNSPAVNALAAPGGSIVIFRGLLEKTTTP
                     EELAGVLAHEMQHILQRHATRALLQHVSMKILLAAAVGDARGLSYGLEGAQTLGMLRY
                     SRQKEEEADREAIKMLITSGIDPRGILTFFETVQKESKKSLKLPSYLSTHPDLEERIQ
                     RLKTLTAHAPIPTIGLLPGYDWRNLTNICPSQQDP"
     gene            3743..>4445
                     /locus_tag="VEL68_13605"
     CDS             3743..>4445
                     /locus_tag="VEL68_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ37049.1"
                     /translation="MMKNFKCWPKFNPRSLNYPEIPVFQILRSTAAKLPFRIAIIFDS
                     LEITYSELLTLCERFANALASLGVKKGDRVAIHLPNSPQFAIAYYATLMNGAIFTPCS
                     PLMVEREMEYQINDAGAETIITLDLFYPTVAKVVPKTKLKNVIVAAIPDIFPPLLTSI
                     KPQLMKKEPPGTLDFSSLLKAYEPKVLEVKIEPKKDLAHLAYTGGTTGVSKGVMLTHF
                     NVVVNVLQSAHWGAGG"
BASE COUNT         1009 a         1185 c         1196 g         1055 t
ORIGIN      
        1 tctgcgttcc tgcagaaaga ggacccccgc gaggcctgcc agcgcatcgc cgagctgggg
       61 cgcaaggttg ccggggtgac cctgggagcg aaggggtatg tagccctggc cgaaggcctt
      121 tggattgaga aacctgccta cccggtagag gcggtggaca caaccgggtg cggggatgtt
      181 ttccatgccg ggtttatctt tggggtgctc agagggtggg cgtacgagaa atgctttgac
      241 ttcgccgcct gggcggcagc catggtgagc ttaaaaatgg gggggaggag cggaatccct
      301 tcccttgaac aggtccggga aagagggttt tagccgtccc gccggggcca gtcaattctt
      361 tggagaaggg aatttttgat cttttcggac ctaagtaaaa ggtatcattc gggggaaaat
      421 atattcccag ctatcaatat tttggcccaa gatagagcta ttggagatag gctatagcgt
      481 ccattctgaa accctatttt tctcctcctt gagagactga actttctttc ctcatgcctg
      541 cgaaggcagg tatccaaaga ctgaaatcct ttattattcc cttacctggt tccccccctt
      601 ttcaggaatc gcgggaaacc caaatttttg acttttgcga gaacgttagt atctggaatg
      661 ggggaggagg ccgaaagccg aaagagccca ctctcttaag tttaagggcc aaacgaccga
      721 caacggttgt agaagttttt tcccagtctc aggacctaga aaagcaaacc ccagaatgga
      781 catccatatt taagggggga aaccatgtac ggagtgaagt gtgccaaatg cggtctcacg
      841 cagcttccta aggaacaatg cgagtcctgt ggcacccgct tagatagttc caagggaccg
      901 gcctatgctc ggcggccaat gactttgagc cctccctcgt tcccttcagc tccgggggaa
      961 atcaatgacc tcactcaatt gattcacgtc ccctcaggag ttagcgtggg cagcgaaagc
     1021 cgacacttgt cctttcatgg gggcgggggg cagctcttcg gaatttgcct gctcaatacc
     1081 cttctgacca ttatcaccct ggggttctac tccttctggg gaaagaccaa ggtccgcgcc
     1141 tacctctggg gaaagaccca gctggaggga gaccgttttg tataccacgg aacaggcagg
     1201 gagctgctga acggtgcttt caaggccatg ctggtcttcg gatttcccct gacccttctc
     1261 aataccttgc cgaaatttct ggagcccagg gaaattttag gagtcgtcgc ctcgatcctt
     1321 acctacgcca tggttatctt gttcatcccg atggccatgg tcggagcccg ccggtacagg
     1381 ttcagccgga cttcctggcg gggaatccat ttttccttcc gcggccaggc ccgggagttc
     1441 ctgaggctct tcttggaagg atgggtcttg accgttctca ccctcggcct ctactacccc
     1501 tttttcgaca tccgtcggca tgggttcatg gtgtccaact cttacttcgg cagcaaacgg
     1561 ttcaagtttg acggagccgg gcgggacttg ttcaaggcct atcttctggc cgtgctcctt
     1621 tccctcccta cgctcggcct atattggttc tggtttatgg ccaagaagca gagatacttt
     1681 tgggagcaca gccatctgga cacactccac ttccggtcta cggtcaccgg agggcgtttg
     1741 ctggcatttt attcgggaaa tcttttcctg ctgattttga ctctgggtct ggcctggtcc
     1801 tgggtggtta tccgtaaggt ccggttcaac tttgcttacc ttaccctgga gggagcgatg
     1861 gatgcagcca aggtccaaca ggaggtccag gcggcctcgg ccacagggga tgcgctggcc
     1921 gggtttatgg atgccagttt tgacctgggg tcataacgac aagttccggg ttgcaggttg
     1981 cgggtttcgg gttctcatta gttggttcaa tcttttcttt ttccgaaatc cgctatccgc
     2041 aaccgaattt ggtctggagt ccaaacgggc gaccggccgg tcgcccttac aatctacgaa
     2101 tttttttacc gatgagcagc gaccttgaag gctactatat ggatggccgg acggcggaac
     2161 ggcaccgggc tggcgtccgg ctgaccccca tgggcttgca gattaccctg gaaaataaga
     2221 attccttttt ctggcccatg gaagaaattc agcaatcgaa gaatttttac ggcgaggaac
     2281 aggttcgact ggaaagaggt ggaaaaaccc cggaagtcct ccttttgccc tcttccattt
     2341 ttctcaagcg aataaaggaa atttccccgg aaaaaaagaa acggtttcat cctccggccc
     2401 gcagaaagag ctgggtctgg gtagttattc tttctgccgc aggggtgctc gggatgactg
     2461 cttctctcta tctatggggg attcccgcta tgacctcctg gatggctccc tatattccgg
     2521 tttcatggga agagcggctc ggccggtctg tagtcgacac cattgctccg gttgaaaaga
     2581 gatgcgagga ttcagcccag tcgaaaaagt tccaggagat tcttaatgcc ctgggctcct
     2641 cggttcccca ttccccctat aaattccggg tgatggtggt gaacagccct gcggtcaatg
     2701 ccctggccgc gccggggggg agcatcgtga tttttcgggg gctgctggaa aaaacgacca
     2761 cccccgagga actggcagga gttctggctc acgagatgca gcacatcctc cagcgtcacg
     2821 ccacccgcgc ccttctgcag catgtttcca tgaaaattct tctggccgcg gcggtggggg
     2881 atgcccgagg gttatcgtat ggtcttgagg gagcccaaac cttgggcatg ttgcggtatt
     2941 cccgccagaa ggaagaagaa gccgaccgag aggcgattaa aatgctcatc acttcaggaa
     3001 tcgacccccg ggggatcttg accttttttg aaacagtgca aaaagagagt aaaaaatcgc
     3061 tgaagcttcc ctcctacctg tccacccacc ccgatttgga agaaagaatt cagcgtctca
     3121 aaactcttac ggcacacgcc ccgattccaa cgattgggct ccttccggga tacgactgga
     3181 ggaacctcac caacatctgt cccagccaac aggacccctg atcgaaatcc attcccaaac
     3241 ctggatcttt cagttcatta tctccgtcca ggacccagga aactctcctg cccctaccgc
     3301 aataaataaa agaatagggg gaagggcatg aaaaaagggg gcatctccaa aatgaggggg
     3361 gcataaatca aaaaaattct tgccaggtta ttcgtccttt tgctatattg ggacggtctt
     3421 gatataaacg gcctcggcag attctgaaaa tgtactcccc taaccacagg gaggatggaa
     3481 aatgcctcgg ttcataaagc cccatttctc tttatcagag ggatggggtt ttttgtttgg
     3541 tcgaggcgat taacgccggg ctggcggctt tccacctgca tggagtaagg agcgttacgc
     3601 ggcaaacgac ttaccaagca agggcctgct tatccccggc cgatccagag aatgacccgg
     3661 ggagtcgatc ggaagctagc ccaggaggcg gcaagggaat ttagaattcg ggacaaaaag
     3721 cggtaaaaaa accttggagg cgatgatgaa aaatttcaaa tgctggccga aattcaatcc
     3781 tcgttccctg aattatcccg aaatccccgt cttccaaatc ttgcgttcca ccgcggccaa
     3841 actgcctttc cggatagcca tcattttcga ttccctggag atcacctaca gcgaacttct
     3901 gacgctgtgc gagcgttttg ccaacgcctt ggccagccta ggtgtcaaaa agggggaccg
     3961 ggtggccatc caccttccca actcgccgca gtttgccatc gcctactatg cgacgctgat
     4021 gaacggcgcc attttcactc cgtgcagccc tttgatggtg gaaagggaga tggaatacca
     4081 aatcaacgat gcgggtgcgg aaaccatcat tacgctggat cttttttacc cgacagttgc
     4141 gaaggtggtt cccaagacca agctcaaaaa cgtaattgta gctgcaattc ccgacatttt
     4201 tccgcctctt ctcacatcca tcaaacccca gctgatgaag aaagaacctc ccgggaccct
     4261 ggatttcagt tccctattga aggcctatga acccaaagtt ctggaggtga agatcgagcc
     4321 caagaaagac ctggcccact tggcttatac cggaggaacg accggagtgt ccaaaggggt
     4381 catgctcacc catttcaatg tggtggtcaa cgtgcttcaa tccgcccact ggggggcggg
     4441 cggca
//
LOCUS       DASPRR010000362         7096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2339161, whole genome shotgun sequence.
ACCESSION   DASPRR010000362 DASPRR010000000
VERSION     DASPRR010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339161"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VEL68_13610"
     CDS             <1..1061
                     /locus_tag="VEL68_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ37050.1"
                     /translation="LPPIQTKADLAALPYSSGTAGLSKGVMITHFNLVSNTIQSMNAV
                     EARGSDIFISFLPFHHIYGFTYFLCGSIYLGASQVVMSRFHAEECLGLMEKYRVTMIF
                     GVQPALLAFLNVPDLERFDLSSLRYIWIGAAPLVPAIARTIQGKFRVPIARHYGLSEA
                     SPTTHSNPPDRPKEGSVGIAVSDVEDKIMDWDKGETEMEVGEPGELAVRGPNLFQGYW
                     KHPEDTRLALRDGWLYTGDIARMDEEGYVFILDRKKEMIKYRGYQIAPAELEAILMEH
                     PAVQDCAVVGIPEEKAGEIPKAFIVKKEGVDLSAEEIMAFVAERVAPYKKVRDVSFIH
                     EIPKNQSGKILRRVLKVG"
     gene            1239..2705
                     /locus_tag="VEL68_13615"
     CDS             1239..2705
                     /locus_tag="VEL68_13615"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ37051.1"
                     /translation="MEILKVFEPLFYPKSLAVIGASSDVTKFGNIILNALMEIGFEGK
                     IYPVNTEGGEINGLKAYRSLQDITGEVDFAVLTIPAPAVKDSLEECRRKQVKGVEILT
                     SGFKETGTPEGWHLEQQIAGFARRGMRILGPNCFGVYCPESGLTILPGQNFSRQTGPV
                     GFLSQSGGLCADLGQMAKGLGIRFSKMVSYGNGCDVAACDLLEYFFADPKTRIIAGYL
                     EGVEDGPRFLKILRENKGKKPVILWKAGLTETGSRAVMSHTGSLSGAAGVWNSGLKQA
                     GVVQVGTAEELIDSIYAFLYLPPNAGPRVALMGGGGAIGVAASDSLDRLKLSVPVFSP
                     PILEKLKASFPPVGNSLKNPVDLGNPMIPPSMLRKVMEAAGEDEGIDTLIVIQILFYI
                     LYQVRHRLGMDDKPLSQFSFQPELLEACREVQERYRKAIILVMPDIVTDGKMIDLEIE
                     WRRERDNYQAAGFPVFKSLDRAARALSQFVIYHQQLQA"
     gene            2737..3624
                     /locus_tag="VEL68_13620"
     CDS             2737..3624
                     /locus_tag="VEL68_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXZ37052.1"
                     /translation="MGLWKSRLERKKILIADGGWGTELQKRGLAPGEPPEAWNLSRPE
                     EVGAVARSYVEAGADIILTNTFGGSPLKLAKVHLEDKLLEINRQGVRISKQGAGDRAF
                     VFASMGPSGEFMAPLGTVSEVDMVQGFAKQARALAMGGADGIVIETMMDLAEAKAALR
                     AAKESTSLPVAVTLTFNKGPKGYATMMGVRPEKATAELERAGADIVGANCGAGIEIMI
                     EIMVLMRSTTTLPIWCKPNAGLPELVHGKTIYRETPEMMASKLKALVQAGAKIVGGCC
                     GTTPSHIRALVLERTKLAD"
     gene            complement(4180..5277)
                     /locus_tag="VEL68_13625"
     CDS             complement(4180..5277)
                     /locus_tag="VEL68_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ37053.1"
                     /translation="MKVTDIQGIMISIPLRKPTSMSNKTVTAREYVVTRVFTDEGIIG
                     SAYTLGGTVVLTAVNDTLKPLVMGEDPFDTERLWDKMFRTSLMVGRKGAVIRALSMID
                     IAFWDIKGRALNQPLYKLLGAYTDRVPVYSSGGYYRQGESFQQMADEMAGIVERGFTS
                     IKIKVGALPFKEEVERLKTLRRTVGDQVEIMVDANCAWNDLSYARKIMRAFEEYEISW
                     FEEPVLPDNFRGGAELAATFETPIATGEQECTRWGFRDLIEHRAADILQPDVAVVGGV
                     TEWMKVAALASAYDLPVASHYFHDVHVHLMASVPNALMAEYFPLDLDIMVFDTVVKEP
                     LKPEKGYLPVPKRPGLGMELDEEKMKRYRVR"
     gene            complement(5327..5896)
                     /locus_tag="VEL68_13630"
     CDS             complement(5327..5896)
                     /locus_tag="VEL68_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ37054.1"
                     /translation="MNRKKILIFMGSPRREGNSTILAQQVAAGAEAAGAQVESFFLHE
                     MNVRPCDACDACRGKTETDCVLDDDMKDIFPKLRQTDGIVIASPIYWFTVSAQTKLFM
                     DRWYALGGPEGYALKGKKFGIVLTYADSDPFTSGAVNALRTFQDALNFVNATIVGMVY
                     GSAWAAGEIKDHREILEKAFELGKKMATG"
     gene            6397..6663
                     /locus_tag="VEL68_13635"
     CDS             6397..6663
                     /locus_tag="VEL68_13635"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37055.1"
                     /translation="METKDGEIIGGTLLYAAYFNAGLRIVDNSDPYHPREVGFYIPAP
                     VALKSARRKMVIQTNDVDLDYRGLVYITDRAGNGLHILEFTGEK"
     gene            complement(6710..>7096)
                     /locus_tag="VEL68_13640"
     CDS             complement(6710..>7096)
                     /locus_tag="VEL68_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37056.1"
                     /translation="PPKPEVLQEMPKPVPPPQTIKQGWDSRGNAWAQERQEGRPLLLT
                     QALNYFVVIGEYDNEKSAWDDYKKWKEKRLRTELYFPKDLGVFEIRKGQYILVYSRHS
                     SQEEANKVFQLLRINDPGLNVKILKY"
BASE COUNT         1620 a         1901 c         1924 g         1651 t
ORIGIN      
        1 cccttccccc gattcagacc aaggcagacc tggctgctct tccttactcc tcgggcacgg
       61 ctggattaag caaaggggtt atgattactc atttcaacct ggtatccaat acaatccagt
      121 ccatgaatgc ggtggaggcc agggggagtg atattttcat cagtttccta cctttccatc
      181 atatttacgg gttcacctac ttcctctgcg gctccatcta cctgggggcc agtcaggtcg
      241 tgatgtcccg cttccatgcc gaagagtgtt tgggcctcat ggaaaaatat cgggtcacaa
      301 tgatcttcgg ggttcagccg gctttgttag cttttctgaa cgtgcccgat ctagaacgat
      361 tcgacctctc ctccctgcgg tacatctgga tcggtgccgc tcccctggtt ccggccattg
      421 cccgaactat ccaggggaag tttcgggtgc ccattgcccg ccattatggt ttgagcgagg
      481 cttctcccac gacccactcc aatccaccgg accgtcccaa agaagggtcg gttgggattg
      541 cagtgagcga tgtggaagat aaaatcatgg actgggataa gggagagacg gagatggagg
      601 taggagagcc tggagagctg gcggtccggg gccctaacct cttccagggg tactggaagc
      661 acccggaaga tacccggctg gccctccgcg atggctggct atacaccggg gacatcgccc
      721 gaatggacga agaagggtat gtattcattc tggaccgaaa aaaggagatg attaagtacc
      781 ggggatacca gattgccccg gctgaattgg aagctatcct gatggaacat ccagcggtcc
      841 aagactgcgc ggttgtgggg atccccgaag aaaaagccgg agagatcccc aaagccttta
      901 ttgtaaagaa agaaggggtt gacctttccg ccgaagagat catggctttc gtagctgagc
      961 gggtcgctcc ttataaaaag gtccgcgacg tgtcctttat ccacgaaatc ccgaaaaatc
     1021 agtccggtaa gatcctgcga cgggttctga aggtcgggta gcttgcggtt tatagcccct
     1081 ttccatgcat tcttgcgaat ttctcaagca tgttttttcc cccaatcgga aatccccatt
     1141 gcccactttt cattccttcc ttgacaagtt tccccttcct tggctatgta ttgaaaatcc
     1201 tggaaaaaaa tccaagcagg agcggaagaa aagtgacgat ggagatctta aaagtctttg
     1261 aacctctctt ttatcccaaa tctctggcgg ttatcggcgc ttcatccgat gtgaccaagt
     1321 tcggtaatat catcctcaac gccctcatgg agatcggctt cgaaggaaaa atctaccccg
     1381 tcaacacgga agggggagag atcaatgggc tgaaagccta ccgatctctc caggatatca
     1441 ccggggaggt ggacttcgcc gttctgacca tccctgcgcc ggccgttaaa gactctttgg
     1501 aggaatgccg ccgcaagcag gtgaaagggg tggagatctt aacttccgga ttcaaggaaa
     1561 ctgggacacc cgaaggatgg catttggaac aacagatcgc cgggtttgcc cggcggggaa
     1621 tgcgcattct cggccccaat tgttttgggg tctactgccc ggagagcggg ttgaccattc
     1681 tacccggcca gaacttctcc cggcagaccg gccccgtagg tttcctctcc caaagcggcg
     1741 gcctttgcgc agacctggga caaatggcca aagggttggg aattcggttc agcaagatgg
     1801 tcagctacgg caacgggtgc gacgtagcgg cctgcgatct tcttgaatac ttcttcgccg
     1861 atcccaagac gaggatcatt gccgggtacc tggagggtgt ggaagacggc cccagatttc
     1921 tgaagattct gagagaaaat aaagggaaga aaccggtcat tctctggaag gcagggctga
     1981 ccgaaaccgg aagccgggca gtgatgagtc atacgggctc cctcagtggt gccgcggggg
     2041 tttggaattc aggcctgaaa caggctggcg tggtccaggt ggggaccgcg gaagagctta
     2101 tcgattcaat ttacgctttt ctttacctcc cgccgaatgc cggacccagg gtggctctca
     2161 tgggaggcgg cggagcgatc ggggttgccg cttcggactc cctggaccgg ctaaaactgt
     2221 ccgttccggt cttctccccg cccattctgg agaagctcaa ggcctccttt cctccggtgg
     2281 gaaacagcct gaagaacccc gtggacctgg gcaatcccat gatccccccc tccatgctgc
     2341 gcaaggtcat ggaggccgcg ggcgaagacg aagggatcga caccttgatc gtgattcaaa
     2401 tcctcttcta cattctctac caggtccgcc accgtttggg gatggatgac aagcctctct
     2461 ctcagttcag tttccagccc gagcttctgg aggcctgccg ggaggtgcaa gaaagatata
     2521 ggaaggccat catcctggta atgcccgata ttgtcaccga tggaaagatg atcgacctgg
     2581 agatcgaatg gcgccgggaa agggataact accaggcagc cggcttcccg gtcttcaaaa
     2641 gcctggatcg cgccgctcgg gccttaagcc aattcgtgat atatcatcag cagcttcaag
     2701 cttgagaggg aatgcttcct cgtggggggt aggaaaatgg gtctttggaa aagccgactg
     2761 gaaaggaaaa aaatattgat cgccgacggt gggtggggaa cagagctgca gaaacgcggt
     2821 cttgccccgg gcgagcctcc cgaggcctgg aacctcagcc gcccggaaga ggtgggcgcc
     2881 gtggcccggt cctatgtgga agccggggcg gatatcatcc tgaccaatac cttcggcggc
     2941 agtcccctga agcttgccaa ggtacatctg gaagacaagc tgctcgagat caatcggcaa
     3001 ggggtaagga tatccaaaca gggagccggg gatcgggcct tcgttttcgc ctccatgggg
     3061 ccgagcggcg aatttatggc ccctttgggg acggtcagcg aagtggacat ggtgcaaggc
     3121 ttcgctaaac aggccagggc cctcgctatg gggggagcgg acggcatcgt gatcgagacc
     3181 atgatggacc tagccgaagc caaggccgct ttacgggcgg ccaaagagag tacatccctg
     3241 ccggtagctg taaccctgac tttcaacaag gggccaaaag gatacgccac gatgatgggc
     3301 gtccgtccag agaaggctac cgcggagctg gagagggccg gggcggatat tgtgggggcc
     3361 aactgcggcg caggtatcga aatcatgatt gaaatcatgg tgttgatgcg ctcgaccacc
     3421 accctgccca tctggtgcaa acccaacgca ggcctccctg agctagtgca cgggaaaacg
     3481 atctaccgcg aaaccccgga gatgatggcc tctaaactga aggccctggt tcaggccggg
     3541 gccaaaattg tgggcggctg ctgtggcaca accccatcgc acatccgcgc cctggtgctg
     3601 gaacgaacaa aattggcaga ttaagccccg ccggatactc gaataaaggg tcaggcttgt
     3661 gattttgtaa tttttaatct ccgccaacat tccagaaaaa ctagtaaaat aaaccaattg
     3721 aaaatctact actctctacg cttgagcata attttttata tcctcgcccc gtcggcaaaa
     3781 atggattcaa gaaagatcaa taacttccat acgctatcgg attagcggtc gagcaaattg
     3841 tagggtctgg aggaatcgag attggttgag tagggggtca ggcttgtaat attgtaattt
     3901 aagatgggga taaaaatagg atttgagaat tttctcaccg ctttcgaaga tctggacgcc
     3961 ggaagtcttg aaccgtggta acctttgggt tcgaagcccc atggagtcct gtagagacaa
     4021 tggataaatg tcagtcaaat cttccctgaa aagatcgcat tttcaagtcc cccgccaatg
     4081 cactttaaaa taggctgatt aatccgaggc cccttttccc tagctcgtat gccttcccta
     4141 taacctcctg gttgtccttg atcttcccgg gaggccattt tacctgactc tgtagcgctt
     4201 cattttttct tcatccagtt ccatccccag gccaggacgt ttggggaccg gcaggtatcc
     4261 tttttccggc ttcaggggtt ctttgacgac ggtatcgaac accatgatgt ccaggtcaag
     4321 cggaaagtac tcggccatta aggcgtttgg gactgaggcc atcaagtgga cgtggacgtc
     4381 atggaaatag tgggaagcta caggcaggtc gtaggccgaa gccagagccg ccactttcat
     4441 ccattcggtg acgcccccca ccaccgccac atcgggctgg agaatatccg ccgcccggtg
     4501 ctcgatcagg tcgcggaacc cccagcgggt gcattcctgc tcacccgtgg cgatcggggt
     4561 ctcgaaggtg gctgctagtt cggctccccc ccgaaaattg tcaggtaaaa ccggctcttc
     4621 gaaccagctt atctcgtatt cttcgaaagc cctcatgatc ttgcgggcat aggagaggtc
     4681 attccaggcg caattggcat ccaccatgat ttcgacctga tcgcctacgg tgcggcgcag
     4741 cgtcttcagc cgctccacct cctctttgaa agggagggct cctaccttaa tcttgatact
     4801 ggtaaaaccg cgctccacaa tcccggccat ttcatcggcc atttgctgga agctttcccc
     4861 ttgtcgataa tacccgccgg aggagtaaac agggactcgg tcggtgtaag ctcccaggag
     4921 tttgtagaga ggttggttca gagccctgcc tttgatgtcc cagaaagcaa tgtcgatcat
     4981 gctcaaggct cggatcacgg ctcctttccg gccaaccatc aggctagttc ggaacatttt
     5041 gtcccaaagc cgttcggtgt cgaaggggtc ttcccccatc accagcggct tcaaggtatc
     5101 atttaccgca gtaaggacaa cggtgccgcc cagggtatag gcagacccta tgatcccttc
     5161 gtcggtgaag acccgcgtaa ctacatattc ccgcgccgtc accgtcttat ttgacataga
     5221 ggtgggcttc cgcaggggaa tggaaatcat tatcccctga atatccgtga ccttcatgat
     5281 cgctctcccg tgggaagtga ttaaccctct tgggctggtc cggctttcac cctgtagcca
     5341 tttttttgcc taattcgaaa gccttttcga gtatttctcg gtggtccttt atctctccgg
     5401 ccgcccaagc gcttccgtag accatgccta caatagtcgc gttgacgaaa ttcagagcgt
     5461 cctggaaagt ccggagcgcg ttcacggccc ctgaggtgaa ggggtcggag tctgcatagg
     5521 tcaggacaat cccgaacttt ttccctttga gggcatatcc ttcggggcct cccagagcat
     5581 accaacggtc catgaagagc ttggtctggg cagagaccgt gaaccagtag atagggctgg
     5641 cgatgacgat tccgtcggtc tggcggagtt tcgggaagat gtccttcata tcatcatcca
     5701 ggacacagtc cgtctcggtt ttgccgcggc aggcatcaca agcatcgcag ggtcgaacat
     5761 tcatctcgtg gaggaagaag ctttccacct gggcacctgc tgcttctgcg ccggccgcaa
     5821 cctgctgggc caggatggtg ctgtttccct ctcttctcgg gcttcccatg aaaatcagga
     5881 tcttttttcg attcatcctc atctccctcc cgtttccaaa aatgtagacc ttagaaataa
     5941 tactccattt catggaagat tggagaaaaa atgaagtagg accgtacgcg aaccacccct
     6001 actcgcgctt tttccaattc tgacatatcc taaaaacatc cattgattcg aagaggagga
     6061 gttaaatgaa taaaggtctt cctcctccag aaaagtagtc gttacgcttt tcatctggac
     6121 ggccctattg acggttcctt ttcccccctt tctccgggat tcattccacc gcccccttta
     6181 acggactgaa agttccgaac tacacccggg gataggggga aatccgcaac ttcctcgttc
     6241 tcagcgaaga atccttcgcc tataactgcc aggagctcag caggcagatg tacatcgtag
     6301 acgtcatgga ggaaaccaac cctgttccgg tggctatttt caaagtcccg gagggtgact
     6361 tctgcaaacg gggcgacgct tcgcccccac tagcctgtgg agaccaagga tggagagatt
     6421 atcggaggga ccctgcttta cgcagcttac tttaacgctg gcttgagaat cgtggataac
     6481 tccgatcctt accatccacg ggaagtaggc ttttatattc ccgccccggt ggccttaaaa
     6541 agcgcacgaa gaaaaatggt catccagact aatgatgtgg acctggacta tcgaggattg
     6601 gtctatataa ccgaccgggc ggggaatgga ctgcatattc tggagtttac aggggagaaa
     6661 tagccatcag ccttcagcca agagaaagcc tcggaaggag gtcttccttt cagtatttta
     6721 atatcttcac gttcaatccc gggtcattga tccttaacag ctgaaaaacc ttgttggcct
     6781 cttcctgcga tgaatggcgg gagtacacca ggatatactg cccctttcgg atttcaaaca
     6841 cgcctaggtc tttggggaaa tagagttctg tccgaagcct tttttctttc cattttttgt
     6901 aatcatccca ggcggatttc tcattgtcgt attcgccgat gacgacaaag tagttcaaag
     6961 cctgggtcag aagaaggggc ctaccttctt gtctctcttg cgcccaggcg tttccccttg
     7021 aatcccagcc ctgcttgata gtctggggcg gtgggacagg cttgggcatt tcctgaagga
     7081 cttcgggttt aggtgg
//
LOCUS       DASPRR010000363         6392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1211502, whole genome shotgun sequence.
ACCESSION   DASPRR010000363 DASPRR010000000
VERSION     DASPRR010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211502"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1393)
                     /locus_tag="VEL68_13645"
     CDS             complement(161..1393)
                     /locus_tag="VEL68_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ37057.1"
                     /translation="MSKGCAYLVTISWVIISTFTPVLAAESVKIGVIQPFTGITALWG
                     DEAKRGIEMAMEKINAAGGVLKKKVELALEDSKGDPSTAVSAAERLIMREKVVALTGV
                     YSSTEALAVLAAVKKYEPLFLLQGGATTKIDRMYGKEPWLFMLLPRSPDYQKNIAEFL
                     NSIQPKPKRIALAYEDTSYGVDHSRVAKEYLTQLGFELVTFEPFKSGALDHSSLVTKI
                     KGAKPDIFYWIGYAGDSILITKQAKELGFSPKMLVDSAGVGLPEFGASLQQDAEYVCG
                     LEVWIPTAKFPASGQYPQFYPRTEDWVAEYKRRFNKDPQWWSAFNYVALITLTTAMNK
                     AGTTDKAKLITALEATDTMTPFGPLKFFKNSFGAIHQSYQDMIVFQWQKGEKVTLWPP
                     KAAGGKLLYPMPAWDKRS"
     gene            complement(1506..2300)
                     /locus_tag="VEL68_13650"
     CDS             complement(1506..2300)
                     /locus_tag="VEL68_13650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXZ37058.1"
                     /translation="MFTPELKGLITEGKIYDLGQPWYPGIPHHPLHPPFVYGLARKHG
                     DVKFEGGGSSANDLFAFGGHTGTHLDAVGHISKDGKLFGGLEAAQEQDSLRGLSRRNI
                     EETPPIISRGVLLDIPALKRVGVLEKGYPISRKDIQDAIAFQGVEIKPGDVVLVRTGW
                     ARYWDHPSLFNDHEGVPGVTLEAARFLVEAGMRLTGTDTTAYEVVPSKSMEVHVFLLA
                     EQGIQIMEMLNLEELARGKVYSFLFVVIPLKIKGGTGSPVRPIAIR"
     gene            complement(2313..3098)
                     /locus_tag="VEL68_13655"
     CDS             complement(2313..3098)
                     /locus_tag="VEL68_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ37059.1"
                     /translation="MSFQNIIFEKDKGIAKIIINRPDKRNALNRETRGEVREALDLIR
                     RDEEIRVLLFRGAGEQSFVAGADLNDLKAFSPLGMYQHMNNLGQKLYNDIENFPLPTI
                     ALINGFCFGGGLELALSCDIRIASENAKFGQTEILLGFIPGGGATQKLPRLIGSGLAK
                     ELMFTGKVIDAREADRIGLVNRTVPLKDLDPEGLAIATKICSLSPVAIRVCKEAINQA
                     AQSSLNAGLAYEVMAETLCFSSEDRSEGIQAFLDKRPAQFKGK"
     gene            complement(3227..4018)
                     /locus_tag="VEL68_13660"
     CDS             complement(3227..4018)
                     /locus_tag="VEL68_13660"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ37060.1"
                     /translation="MEDFTPFEMMILTAAKQIKNGDALFIGFHWPMVASRVARRLHAP
                     EIYCVYEGGGVERTYCPVMPNTGADLILSPHLAFAGETYDTLCGFLAAGHLPLSMIDA
                     PMVDRFGNINSTCIGPYQHPKARLAGSGGAADLGANSRKLFMLSDRTTKDGFPARVDY
                     VTTPGYLDGFDSRIKAGFRPDTGPEMIISSLGVFKFDPVSKEAYLWGVYPGAELEKIR
                     TAVGWELKVADKLVAIPAPKAEEMRVVREELNIAESRLWKIPGRK"
     gene            complement(4018..4920)
                     /locus_tag="VEL68_13665"
     CDS             complement(4018..4920)
                     /locus_tag="VEL68_13665"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ37061.1"
                     /translation="MNKLITMKAAVAEHIKDGQSFLISGFSINQGYAFAHEIIRQKKK
                     DLTLIRSSGDILAVQLIGAGCLKRVISPYVWNPTGPSSAYCFRRAIEKGIPHPIELEE
                     FSFGSFCMAIYAAAHGLPFMPTYPLEGTGIFQHRSILGADKVRVIESPFSGEKVCLVK
                     PLAPEICLVHVQRADPEGNAQVWGPLGDIRATANAGKKVIVSAEEIADSSAILKEPYK
                     TILPGFRVSAVVHEPWGAHPSDVFEHYYRDIWFQAAYAEASKTVEGFQKFLEEWVYGV
                     ENRAEYLKRLGAKKQGELRWRKEE"
     gene            complement(4971..>6392)
                     /locus_tag="VEL68_13670"
     CDS             complement(4971..>6392)
                     /locus_tag="VEL68_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ37062.1"
                     /translation="WLPNGIEWVVAVFSLANLGAVFVPVNTRFKAEELEYILKQSEAS
                     ALILQDRFQKAEYVNMLGQLCPELPASSPGNLLSARLPALKAIIAVCSHEIPGAYSYR
                     ELFQMGRKIDLKPLEEAVRPAQTVSILYTSGSTAFPKGVMLTHNNILRNGFEIGERLG
                     LTREDRYFNPCPFYHNAGLVDGLLAALTHGSCNVTLPSFDARESLAVMEREKCTAVAG
                     IQTMYAKMMEEPGLDRSRLSLRTGWTTGPPQTPRDIYEKMGVTGITNLYGISEASPCC
                     SISDCRRDPLEDRLNRMGKPLPGVEMKIVNPQSGESLPPNERGEICVRGWNLMQGYYK
                     KPEETAKAIDGEGWLHTGDLGFIDEKGLVFFMGRIKNVVRSGGENISPEEVENFIFRH
                     PKVKHVEVVGLPDEKWGQRVTACIELKEGMEATPEEIITFCKERMANFKVPKEVHFVT
                     DWPMTGSGKVQKFKLVESLLKKT"
BASE COUNT         1339 a         1847 c         1694 g         1512 t
ORIGIN      
        1 gttgccagtt ccagaactct cagtccggat aaggggtttg attttttctc atccatgttg
       61 attcgtataa ggtctttatc cgatcatggg cttttggcgg agtttgtact attaagggtt
      121 cccctagcgg aatgggaaaa ttcggatcaa aaaaatgtct ttatgaacgc ttgtcccagg
      181 ccggcatagg gtacaagagc ttgccgcccg ctgctttagg gggccagaga gtcaccttct
      241 cacctttctg ccactggaag acaatcatat cctggtagct ctggtggatg gcgccaaaac
      301 tattcttaaa aaactttagg ggaccaaaag gagtcatcgt gtccgtggct tccaaagcgg
      361 tgatcaattt cgctttgtcc gttgtgccag ccttgttcat ggccgtggtc aaggtgatca
      421 gggcaacata gttgaaggcc gaccaccact ggggatcctt gttaaacctc cgcttgtatt
      481 cggctaccca atcctccgtc ctggggtaga attgcgggta ttgcccagag gccgggaact
      541 tggcagttgg gatccagact tcaaggccgc agacgtattc tgcgtcttgc tgcagagagg
      601 ctccgaattc gggcagaccc acccccgcag aatctacgag catcttaggg gaaaatccta
      661 actctttggc ctgtttggtt atgagaatgg aatcgccggc atagccaatc cagtaaaata
      721 tgtcgggctt cgctcccttg attttggtaa cgagggaaga atggtccaga gctccacttt
      781 taaaaggttc aaaggtgacc aattcaaagc ccagttgggt caaatattcc ttcgccaccc
      841 tggaatggtc caccccgtaa gatgtgtctt cataggccag agcgattcgt ttaggtttcg
      901 gctggatgct gttcaagaat tcagcgatat ttttttggta atccggtgac ctgggaagga
      961 gcataaaaag ccagggttct tttccataca ttcgatcaat tttcgttgtg gccccacctt
     1021 gaagcagaaa aaggggttcg tattttttca ccgcagccag gacagcaagg gcttccgtgc
     1081 tggaataaac accggttagg gccaccactt tttcccgcat aattaggcgt tcggccgcag
     1141 aaactgctgt cgaggggtct cccttactgt cctccagagc caactccact ttctttttga
     1201 gaacgccgcc ggccgcattg attttttcca tggccatttc aatccctcgc ttcgcttcat
     1261 ctccccagag agcagttatg cccgtgaatg gctggataac cccgatcttc acggactctg
     1321 cggccaaaac aggagtgaaa gtggatataa tgacccatga gatcgtaacc aaataagcac
     1381 agcctttaga catgccaacc ccctttctta gacagggcat cccgaacaac ttccatcccg
     1441 ttgctggaat agcctttcca tgtccttttt ccggagggct taattttaac attaaaaaaa
     1501 cttccctacc gtatagcgat aggccggacg ggagaaccgg tcccgccttt gattttcaac
     1561 ggaatgacca cgaataagaa agaatagact tttccccggg ccagttcctc caggttgagc
     1621 atttccatga tctggattcc ctgctcggca agcaagaaga cgtgcacctc catggatttc
     1681 gagggaacga cctcataagc cgtggtatcc gtcccagtca gacgcatccc tgcttcaacc
     1741 agaaatcgcg ccgcctcgag cgtcaccccg ggcactcctt cgtggtcgtt aaaaagactg
     1801 gggtgatccc agtagcgcgc ccatccggtt ctgaccagga ccacatcccc cggcttgatc
     1861 tccactccct gaaaggctat cgcgtcctgg atgtctttcc ggctgatggg atatcctttc
     1921 tccagcaccc ccactctctt gagcgctggg atatccagaa ggaccccgcg ggagatgatt
     1981 ggtggagtct cctcgatatt tcgtcgagat aatccccgca aagaatcctg ctcctgggct
     2041 gcttccaagc ctccgaataa ctttccgtcc ttcgagatgt gcccgaccgc atccaggtgg
     2101 gtgcctgtat gccccccgaa agcaaagaga tcgttggccg agctccctcc cccttcgaat
     2161 tttacatctc catgtttcct ggccagcccg tagacaaacg gagggtgtag aggatggtgg
     2221 ggaattcccg gataccaggg ctgccccagg tcatagatct tcccttcggt gatgagacct
     2281 ttcagctccg gcgtaaacat ggcatccttc ccttattttc ccttgaattg agcggggcgc
     2341 ttgtccagga acgcctggat tccctcggac cggtcttcgg aagagaaaca gagcgtttcc
     2401 gccatgactt cataggccag tcctgcgttc agggaagact gggcagcctg attgatggcc
     2461 tccttgcaga ctcgaatcgc aaccggggaa agagagcaga tttttgtagc gatggccagc
     2521 ccctccgggt ccaggtcctt cagggggacg gtccggttga ccagtccgat ccggtccgct
     2581 tcccgagcat cgatgacctt cccggtgaac atgagttctt tggccagccc ggagcctatg
     2641 agccggggca gtttttgcgt ggctcccccg ccggggatga atcccaggag gatctcggtc
     2701 tgcccgaatt tggcattctc cgaggcgatg cggatgtcac aggaaagggc cagttcaagc
     2761 ccgcccccga agcagaaccc gttgatcaag gcgatggtgg gaagaggaaa attctcgata
     2821 tcgttgtaca gcttttggcc caggttgttc atgtgctggt acattcccag gggggagaaa
     2881 gccttcaggt cgttcaggtc cgctccggcg acgaaagatt gttctccggc cccccgaaag
     2941 agcaaaaccc ggatttcttc atccctgcgg atcaggtcca aggcttcccg gacttccccg
     3001 cgggtctccc ggttcagggc atttcgtttg tccggccggt tgataataat tttggcgatg
     3061 cctttatcct tctcgaagat tatgttctgg aaactcatga atccctccca gtaaaagttt
     3121 caggttgcag gtcccaggct acaggccaca ggcttttttg cagcgtgcga cttggggctt
     3181 gtggcttgcg acctcggtca gctttgcacc ctcagcggta aatctttcat tttcggcctg
     3241 ggattttcca taaccggctt tcagcgatat tcagctcctc ccggaccacc ctcatctctt
     3301 cagccttagg cgcagggatg gccacaagtt tgtccgcaac tttaagctcc caccccacag
     3361 cggttctgat cttctctagt tcggcgcctg gataaactcc ccagaggtag gcctccttgg
     3421 agacgggatc gaacttgaac acccccaggg aggagatgat catctccggc cctgtatccg
     3481 gccggaagcc cgccttgatc cggctgtcaa acccatccag ataccctgga gtggtgacgt
     3541 aatccacccg ggccgggaag ccatctttgg tcgtccggtc ggatagcatg aaaagttttc
     3601 tggagttggc cccgaggtcc gcggccccgc cggagcctgc cagcctagct ttgggatgct
     3661 ggtagggacc gatgcaggtg gagttgatgt ttccaaaccg gtccaccatg ggagcatcga
     3721 tcatggaaag gggaaggtgt cctgcagcca ggaacccgca gagggtgtcg taggtctccc
     3781 cggcgaaggc cagatggggg ctgagaatta aatcggcgcc agtattgggc atgaccgggc
     3841 agtaggtcct ctccaccccg cccccttcat acacgcaata aatttccgga gcatggaggc
     3901 gtcgggcgac gcgggaagcc accatgggcc agtgaaaacc gatgaataaa gcgtcgccgt
     3961 ttttgatctg cttcgccgcc gtgaggatca tcatctcgaa aggagtgaaa tcttccatta
     4021 ttcttccttc ctccaacgca attccccttg tttcttcgct cccagccttt taaggtattc
     4081 agcccggttc tccaccccgt acacccattc ctccaggaac ttctggaacc cctccaccgt
     4141 cttcgaggct tctgcgtagg ccgcctggaa ccagatgtcc cgatagtaat gctcgaatac
     4201 atccgatgga tgggcgcccc agggttcatg caccacagcg ctcacccgga atcccggaag
     4261 gatggttttg tacggctcct tcagaatggc cgacgagtcc gcgatctctt ctgcggagac
     4321 gatgaccttc tttcccgcgt tggccgtcgc ccggatatct cccaagggac cccagacctg
     4381 ggcattccct tccgggtccg cccgttgaac atggacaaga cagatttccg gggctagcgg
     4441 cttgaccagg cagactttct cccccgagaa cggcgattca atgacccgga ctttatccgc
     4501 ccccaggatg gagcggtgct ggaagatccc ggttccctcc aaaggatacg tgggcatgaa
     4561 gggcaacccg tgcgcggcgg catagatggc catgcagaac gagccgaacg agaactcctc
     4621 cagttcaatc gggtggggga tccctttttc tatcgcccgg cggaagcagt aagctgagga
     4681 aggcccggtc gggttccaaa cgtaaggaga gatgaccctt ttgaggcatc cggccccgat
     4741 caattgaacg gcgagaatgt cccctgagct gcgaatcagg gtcaagtcct tcttcttctg
     4801 gcgaatgatc tcgtgggcaa aggcatatcc ctggttgatg gaaaacccgg aaatcaagaa
     4861 gctttggccg tcctttatgt gctcggcgac ggccgctttc atggttatta gcttgttcac
     4921 ggatgtttcc cttccattcc tgcccgtagg ggcgacccgc cgggtcgccc ctacgtcttt
     4981 tttaataggc tttccaccag tttgaacttc tgtaccttgc cgctcccggt catgggccag
     5041 tcggtcacaa agtgcacttc cttgggtacc ttgaaatttg ccatcctctc tttgcagaag
     5101 gtgatgatct cttccggggt ggcttccatg ccctctttca attcaatgca ggcggtcacc
     5161 cgctgtcccc atttttcatc cggaaggcca accacctcca catgcttcac tttggggtga
     5221 cggaagataa aattctccac ctcttccgga gaaatgtttt cccctccgct ccggacgaca
     5281 ttcttgatcc ggcccatgaa aaagaccagc cccttctcat cgataaaccc caggtctccc
     5341 gtatggagcc atccttctcc atcaatggcc ttcgccgtct cttcgggctt tttataatac
     5401 ccctgcatga gattccaccc gcggacacag atctcccccc tctcgttggg cggcaggctc
     5461 tccccgcttt gagggtttac gatcttcatc tccactccgg gcagcggttt ccccatccga
     5521 ttcaaccggt cttccagcgg atcacgccgg caatcggaga tggagcagca gggggaagcc
     5581 tcagagattc cgtagaggtt ggtgatcccg gtcaccccca tcttctcgta gatatcccgg
     5641 ggggtctgcg gcgggccggt cgtccacccg gtccgaaggg ataagcggct ccggtccagg
     5701 cccggttctt ccatcatttt tgcatacatg gtctggattc cagcgacggc cgtgcatttc
     5761 tcccgttcca tgaccgccag ggactcccgg gcatcgaagg atggaagggt cacgttgcag
     5821 ctgccatgcg tcagggccgc caaaagcccg tctaccaatc cagcattatg gtaaaaggga
     5881 caggggttga aataacggtc ttcccgggta agcccgaggc gctcgccgat ctcaaaaccg
     5941 ttccgtagga tattattgtg ggtcagcatg acccctttgg gaaaagcggt ggacccggag
     6001 gtgtagagga tgctcaccgt ctgggccggg cgtacagcct cttcgagagg tttgaggtcg
     6061 atcttccgcc ccatctgaaa aagctcccta taggaatatg cccctggtat ttcatgggag
     6121 cagacggcaa taatcgcctt aagggcggga aggcgggcgg aaaggaggtt tcccggagag
     6181 gaggccggga gttcagggca aagctgcccc agcatattca catattccgc cttttgaaac
     6241 cggtcctgaa gaatcagagc agaggcttcc gactgtttga ggatgtattc cagctcctcc
     6301 gccttgaatc gggtattcac cgggacgaag acggctccca gattggccag ggagaaaacc
     6361 gccaccaccc attcgatccc gttgggcagc ca
//
LOCUS       DASPRR010000364         5661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_234665, whole genome shotgun sequence.
ACCESSION   DASPRR010000364 DASPRR010000000
VERSION     DASPRR010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234665"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..1102
                     /locus_tag="VEL68_13675"
     CDS             467..1102
                     /locus_tag="VEL68_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259447001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:509..511,aa:Sec)
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HXZ37063.1"
                     /translation="MEKPKKLEVFSDYIUPWCYFITGRIEKLKKNFDIEVQWRAFPLH
                     PETPEEGRALEELFAGRNMDIPAMLARLKSVAEAEGLPWGTRKMTFNSRLAQEMGKWA
                     EEKGKGDEFHNAVFRAYFVNGKNIAKKEVLLELGKKMGLPVKEAEEVLESRPFKAAVD
                     EDWKLSIRRGVTAVPTFVVDHQVVVGAQPYEVLEQFLKNNGVRKKGASRKA"
     gene            1670..2530
                     /locus_tag="VEL68_13680"
     CDS             1670..2530
                     /locus_tag="VEL68_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXZ37064.1"
                     /translation="MTAFALALVLSAAFIHAGWNFLAKRAGEGGAAFVWLFAVLSSIF
                     YAPLAVFIYFWQKPHIGPLQLGFMVGSALIHVAYFLILQRGYRVGDLSLVYPLARGTG
                     PTLSTFSAILVLGERPTPLAIAGALLVAAGVFFLAGMAANSKGRRKWGVGYGLLTGAI
                     IAAYTLWDKYAVSTLLIPPLLQDYCTTLARVIMLGPVALRKWGDVQREWRIHRKEALG
                     VALLCPLSYIMVLTALITAPVSYIAPAREVSILIGAIMGSRLLAEGDARRRLPAAAAI
                     VAGVIALAVG"
     gene            complement(2810..3187)
                     /locus_tag="VEL68_13685"
     CDS             complement(2810..3187)
                     /locus_tag="VEL68_13685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXZ37065.1"
                     /translation="MRRNADSDRKLKDLGKVDGRQHPRVLLFLPVEYRGNSPKSLRLG
                     HTSDLSRDGVMLNLRERLSIGQRINLSIFVSLGKAVEAIKVNSQVVWVHDAGKDGNFR
                     SGVKFIDLSASDRKKLEEFFEEY"
     gene            complement(3704..4081)
                     /locus_tag="VEL68_13690"
     CDS             complement(3704..4081)
                     /locus_tag="VEL68_13690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ37066.1"
                     /translation="MIIARSSESKGFLTPAPHRRELKVLISPSVQADVEGLAVGMTIL
                     PPGESSSFHAHEKECETWIIVSGQGEVRVGDERQAVEPETVIFLKPHIPHQITNTGRI
                     PLRMFWVYTPPGGEKSILEGVMK"
     gene            complement(4078..4719)
                     /locus_tag="VEL68_13695"
     CDS             complement(4078..4719)
                     /locus_tag="VEL68_13695"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXZ37067.1"
                     /translation="MQKLVSPSSIGCLEIHYPGLDQLAEVYADLDRQIAEFKRSAGIE
                     CLRKCALCCTTAKFVEASILEMLPLSLHFWEEGRAEELLEKLEGIHPEAPCLLLNADP
                     SDEFGGGCSYYRFRPLVCRLFGFSATVDKSGHPRVVICKPLKKMDRGILDRMNERIRE
                     GLPVPLMPDFSRRVAFLHPHFGQEKYPVNFALREALEIVGRRIHFLKSTRRET"
     gene            complement(4892..>5661)
                     /locus_tag="VEL68_13700"
     CDS             complement(4892..>5661)
                     /locus_tag="VEL68_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HXZ37068.1"
                     /translation="RLRRIAERVEKEGRLPLEAGEFRPMAAETSSDAPLPGKKLAGED
                     ARFEVLRQDLYEGLDGEIKGHILELLALKVNPQEILNRGLLAAMESIGQEFKSGRLFV
                     PEVLLAARAMNEGLSVLEPHLSSSGRQARGKALIGTVQGDLHDIGKNMVIIMLRGVGF
                     DVVDLGINVSADDFLRKVAEEKPHILGLSALLTTTMPQMKKVIEGLAKTGLRDKVRVM
                     VGGAPVSPTFARQIGADGYAADAGEAVEVAKRLVAGS"
BASE COUNT         1272 a         1467 c         1600 g         1322 t
ORIGIN      
        1 ctggattccg ttgtttttct cggattttcg aatactgtcg accccatttt attaggcatc
       61 catatagcct attctttaaa tcatcatttt ggaagaaata tatttgtttt gaaagagatc
      121 tagcaagaaa tattccatcc ccccagagag caataataga gaaatcattc ataaattcaa
      181 tgcattaaag cgaaggccca tagcaagtta agcacctccc tcggaattct gtcttgtgaa
      241 agaggacaaa ttacactgtg acaggatctt atcccatcat ggagaataaa ataagggcga
      301 gttttaaatg aaatagattc cgaaagcatt ggaggcaata ttaaagagat cccgaagggt
      361 acccgccgac aatcgaaccc ggaactaatt ctggagaatc ctggataatc ggcatataat
      421 tgaaccaggg aaacgttaaa tgagaaaaaa tggaggaaga aacgggatgg aaaagccaaa
      481 gaagctggag gttttttcgg attacatatg accctggtgc tatttcatta ccgggcgtat
      541 tgaaaaactg aagaagaatt ttgatatcga agtccagtgg agggctttcc ctcttcaccc
      601 ggagactccg gaggaaggac gggccctgga agagctgttt gccgggcgga acatggatat
      661 tcccgccatg ctggcgcgcc tgaaaagtgt cgccgaggcg gagggccttc cctggggaac
      721 gcggaagatg acctttaaca gccggctggc ccaagagatg ggcaagtggg cggaagagaa
      781 aggcaaaggg gatgagttcc acaatgccgt cttccgggcc tattttgtta acgggaagaa
      841 tatcgccaaa aaagaagtgc ttttggaact gggcaaaaag atgggactgc cggtgaaaga
      901 agcggaagag gtcctggaat caagaccctt caaggcagcg gtggacgagg attggaagct
      961 ttccatccgg agaggagtga ccgccgttcc gacctttgtc gttgatcatc aggtggtagt
     1021 cggggctcaa ccctatgaag ttttagagca gttcctgaaa aacaacgggg tcagaaagaa
     1081 gggcgcctcg cgaaaagcat gactcgtgga gggtagcgac tctggcgcga cgctccttgc
     1141 ccacccccag ttcataaaaa agagtccttc gaaaataaga tctctgaaaa ggagggggaa
     1201 tctcatgggg ttcagagcgg agaaatttgg atcatcatcc tgaggcggtg gttcttccat
     1261 tggatcgggc ctgtcctggg gggattgaaa cgcaaaatgc tttggctctc ttttctgctt
     1321 ggaggtatcc cctctcaggc ctctccccgc ggaagatggg gaggagtttc atggagggat
     1381 aagcacccgc cgagcctgat gatccaaatt cctccaccct gaaccccaac cagcacgaaa
     1441 aactggggtg ggcaagcgcg acgctcctat tgacgaagtc aagacccggg tagtatatta
     1501 ggctatcaac cgacgggtag ctagggttcc gcggattgat atccggcccg gaccgagcgc
     1561 tacacaaagc ccgcagcggc atgcaccttg gggaataaaa ggccagaggg gataggttga
     1621 gacggattct tgtgtctctt cgctatcctt tttggttttc ggaggtgtca tgaccgcttt
     1681 tgctctggcg ctggttctct ccgccgcctt catccacgcc ggctggaact ttctggccaa
     1741 gcgcgccggc gaaggcgggg ccgctttcgt ctggctcttt gctgtgcttt cctccatttt
     1801 ttatgccccc cttgcggtct tcatctactt ctggcaaaag cctcacatag gtcccttgca
     1861 gcttggattc atggtgggca gcgccctgat ccatgtggcc tactttctca ttctccagcg
     1921 aggctaccgg gtaggggatt tgtccctggt ctatcctctg gcccggggaa ccggtcccac
     1981 gctctccacc ttttccgcga ttctcgtgct gggagagagg ccgactcctc tggccattgc
     2041 cggtgcattg ctggtggcgg ccggggtctt ttttcttgcc ggcatggctg caaattccaa
     2101 ggggagaagg aaatgggggg tgggttatgg gctcctcacc ggggcgatca tcgccgccta
     2161 caccctctgg gataaatatg cggtcagcac cctgctcatt ccgcctttgc ttcaggacta
     2221 ctgcaccacc ctggcccggg tcatcatgct gggcccggtc gcccttcgga agtggggaga
     2281 cgtgcagagg gaatggagga ttcatcggaa ggaggctctc ggcgtggctc tattgtgccc
     2341 gctttcctat atcatggttt tgacggcgct cattaccgca ccggtcagct acattgcgcc
     2401 tgcgcgggag gtcagcattt tgatcggggc gatcatgggg tctcggctgc tggccgaagg
     2461 agatgcccgc cggaggctcc cggcggcagc ggcaatcgtg gcgggggtga ttgccctggc
     2521 ggtagggtga aacgattgcg gatttcggat tgcagattgc ggaatgaaag acaaaaagaa
     2581 atggggaatg cggattgcga atttcggaat gaaagataaa gggaaggaaa tgcgagattc
     2641 ggaatggggg ttgtggagag aggaatgaaa ggagattttc agcagctaat acaaacgaag
     2701 taaatgtagt agagtcattg cgagcccgaa gggcgcggca atctcgtttt tgtttgagat
     2761 tgcttcgtcg ctccgctcct cgcaatgaca cgttatttat ttccttgtgt taatattctt
     2821 caaaaaattc ttccagcttt ttccggtcgc tggcggatag gtcgatgaat tttactccag
     2881 accggaagtt tccgtccttg cccgcatcat gaacccaaac aacctgagaa ttgaccttga
     2941 tggcttccac ggctttgccc aaggaaacaa aaatggacag attgatcctt tggccgatac
     3001 tcaacctctc ccgaaggttt agcataaccc cgtcgcgact gaggtcggag gtgtggccga
     3061 ggcgaaggga tttcggacta ttcccccggt attcaacggg gagaaataaa aggaccctgg
     3121 gatgttgtct cccgtccacc ttacccaagt cctttaattt tctgtccgag tcagcatttc
     3181 tgcgcataat gtttcctcgc ctcttccttt tcgggagccg gtgattgaaa atccacagcg
     3241 cgccaatcga gagagcaaac tcaatgccca aaatccagca atgatttcat tcatgatatt
     3301 ggggagatcc tttccatatt agcgcgcagc gggactgtaa tgacaaatta taggcatgcg
     3361 aaggcgttat tataaagcaa gtcttttcag ccctgcaagt cttaatattc ttgggtcggc
     3421 gagcctccag ggggtgcgtt ccaaccttgg cgggacagaa tgaggcgcga atctttcatt
     3481 ggttcgggtc agcaaattct ttggttgtcg ggacctgagc caggattcgg ttttccaaaa
     3541 gggggtcggg gacaggtgca ttgcggaacc caagcatcgc tccggaatcc ggatttttcc
     3601 cgccgattcc gtcccgccaa gcttggaagg caccccctgc gctgagaata tttctggttc
     3661 cgttccaccc actcgtttgg gagaaggccc aatattctgg gcctcatttc attacccctt
     3721 ccaaaatgct tttctctcct cctggcgggg tgtaaaccca gaacatgcgg agaggaatcc
     3781 ggccggtgtt ggtaatttga tgggggatgt ggggtttcag gaagatcact gtctcgggct
     3841 ccaccgcctg gcgttcgtct cccacccgaa cttcgccctg gccggagaca atgatccagg
     3901 tttcacattc tttttcatgg gcgtgaaagc tgctagattc tccgggagga aggatggtca
     3961 tcccgacagc taggccctcc acgtcggcct gcacgctggg cgagatcaaa accttcagtt
     4021 cccgccggtg gggagcaggg gtcaagaaac ctttgctttc gctggacctg gcgatgatca
     4081 tgtttccctc ctcgtggatt tgaggaaatg aatcctccga ccgactattt ccagcgcctc
     4141 ccgaagggca aaattaacgg gatatttttc ctgcccaaaa tgaggatgga gaaaagcgac
     4201 tctgcgggaa aagtcgggca tgaggggaac gggaaggccc tcccggattc tttcgttcat
     4261 ccggtcgagg attcccctat ccatcttttt caagggcttg cagatcacaa cacgggggtg
     4321 gccgctcttg tccacggtgg cggagaaccc gaagaggcgg cagacgaggg gccggaagcg
     4381 gtagtagctg cacccccctc cgaactcatc cgagggatcg gcattcagaa gaaggcacgg
     4441 ggcctccgga tgaatccctt ccagtttctc caaaagctct tccgcccggc cctcttccca
     4501 gaagtgaagg ctcaacggga gcatctccaa aatggaggct tcgacgaatt tagccgtcgt
     4561 gcagcaaagg gcacacttcc tgaggcattc gattcctgca ctcctcttaa actcggcgat
     4621 ctgtcggtcc aagtctgcgt agacctcggc gagctgatca agcccgggat aatgaatttc
     4681 caggcacccg atagaggatg ggctgactaa tttttgcatt tactttctgc tctttacccg
     4741 tgcggttctc tgcaagggtc aggggtgata gccacccgca ggcatttgat gctggttaaa
     4801 gaaaagggcg ttgctttttg ctggttactt gatgctggag aaaaaagacg ttgctgggta
     4861 aaggcaaaac cttaaagcca gcaaccagta actaggaacc agcaaccagc ctcttggcca
     4921 cttccacggc ctccccggca tcggccgcgt atccgtcagc gccgatctgg cgggcgaagg
     4981 tggggctgac cggggcgccc cccaccatga ccctcacttt atctcgaagt ccggttttgg
     5041 ccagcccctc gatgaccttt ttcatttggg gcatggtggt ggtgagaagg gcggacaacc
     5101 ccagaatgtg gggtttctcc tcggcgacct ttctcagaaa atcatcggcg gagacgttga
     5161 tgcccaagtc gacgacgtcg aatcctactc cccgcagcat gatgatgacc atgtttttcc
     5221 cgatgtcgtg gaggtccccc tggactgtgc cgataagcgc ttttccccgg gcttggcggc
     5281 ccgaggaaga gagatggggt tccagcacgg aaaggccctc gttcatggcc cttgccgcca
     5341 agaggacttc gggcacgaat aaccggccgc tcttaaactc ctggccgatg ctctccatgg
     5401 ctgccagcag gcctcggttc aaaatttcct ggggatttac tttcagggcg aggagttcca
     5461 ggatgtgccc cttgatttct ccgtccagac cttcatagag gtcctgcctg aggacctcga
     5521 accgggcgtc ctctccggcc aatttcttcc cgggcagggg cgcatcggag gaagtctcgg
     5581 cggccatcgg gcggaactcc cctgcctcca ggggcaaccg cccttccttc tccacccgct
     5641 cggcgatccg gcggagacgg t
//
LOCUS       DASPRR010000365         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_569787, whole genome shotgun sequence.
ACCESSION   DASPRR010000365 DASPRR010000000
VERSION     DASPRR010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569787"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..724
                     /locus_tag="VEL68_13705"
     CDS             107..724
                     /locus_tag="VEL68_13705"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXZ37069.1"
                     /translation="MIYEIRTYRLAPGSVAEVEKRFGEAYEHRKKYSPIVAFWHTEIG
                     PLNEIIHVWPYQDLGERARVRAESAKDPNWPPKISDFVLSMHSEILVPFPFSPQLTPG
                     KMGPFYEMRIYTIKTGTLPDIMKAWEPKLPERMKLSPLAVAGHVDLGEVNRFIHIWPY
                     NSLDQRERVRSTARQAGVWPPGGGAGRLLTMANKIMMPSAFSPMQ"
     gene            1202..2962
                     /locus_tag="VEL68_13710"
     CDS             1202..2962
                     /locus_tag="VEL68_13710"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXZ37070.1"
                     /translation="MKTAIGLLIKMGVVILSVTGFLTPLAKGADPPVALTPVISGLTQ
                     PVHITHAGDGSGRLFLVQQTGQIKIYQNGALAPVPFLDISSRISCCGEQGLLSIAFPP
                     QYAQKKYFYVSYTNTSGNSVISRFSLTVNPDVADPATEQILLTITQPFSNHNGGLMAF
                     GPDGFLYIGFGDGGSGGDPNNFAQNLTDLPGNQKLLGKMLRIDVESGTQPYAIPPTNP
                     LLNGVRSEIWALGLRNPWRFSFDRQTGDLYIGDVGQASFEEIDFQPAASPGGQNYGWR
                     LMEGNHCFNPPTNCNDGTLTLPVHEYDHTQGDCSVIGGFVYRGTRYVSMQGVYFYGDL
                     CSGRIWGLRNQGGTWQNQLLIDSSLSITTFGEDENGEIYVADYAAGIIYRMVIAGRLG
                     IFRQGSWFLDSSDYGVWEGCVLDSCLGPFGFPSDVPVVGDWTGTGTSKIGTFRNGQWF
                     LDLNGNGTWDGCGTDACIASFGLPTDIPVTGDWTGSGTSKIGTFRNGEWFLDLNGNGT
                     WDGCGTDACITSFGLPTDIPVTGDWTGSGTTKIGVFRNGAWFLDLNSNGVWDGCGVDL
                     CIPSFGGFPGDLPITGKPGS"
     gene            2998..>3344
                     /locus_tag="VEL68_13715"
     CDS             2998..>3344
                     /locus_tag="VEL68_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37071.1"
                     /translation="MAGNERLIKIFEHALQQEFTGRDFFKSSLQRMGWGSAVSAFRRL
                     IQEEEKHIEMIEKILSGLKKGSPIDLSPAKSPAEGPFNHFEERAKKEFFAQCLEGSMV
                     PEVTIFNTAWLIE"
BASE COUNT          759 a          940 c          920 g          725 t
ORIGIN      
        1 gaaagaacta gaattccttg acacaaaagg cccttccctg taaactccca ccacagagcc
       61 aacaagggag ttcaccttaa attcatcgca cgaaaggacc aagaccatga tctacgaaat
      121 ccgtacctac cgcctcgccc cagggagtgt ggccgaggtc gagaagcgct tcggtgaggc
      181 gtacgagcac cgtaaaaagt attctccaat cgttgccttc tggcacaccg agatcggccc
      241 gctgaacgag atcatccacg tctggccgta tcaggacctg ggagagcgtg cccgcgtccg
      301 ggccgagtcc gcgaaagacc cgaactggcc gcccaagatc tccgacttcg tcctctccat
      361 gcactctgaa atcctggttc ccttcccgtt ctctccgcag ctcacaccgg gaaagatggg
      421 accgttctac gagatgcgca tttacacgat caagaccggt actcttcccg acatcatgaa
      481 ggcttgggag cccaaactgc ccgaacgaat gaagctctcg ccccttgccg tcgccgggca
      541 tgtggacctt ggcgaagtta accgtttcat tcatatctgg ccctataaca gcctcgatca
      601 gcgcgaaagg gtccggtcca cggcccggca ggccggcgtg tggccgccgg ggggaggcgc
      661 cgggcgcctg ttgaccatgg cgaataaaat catgatgcca tccgcttttt ccccgatgca
      721 gtagcgaaaa agaggaaccc tgccccgacc ggtgctcttt gcgcgaatct ccttgccccc
      781 cgggcacgaa cgacccaagc acgcgggaaa tatcaatcct ccacaggccg acccccggca
      841 gagccggggg ttcacttaag taaattattc gagggcagac aaaaagggta aggcattttt
      901 gtccccttcc tcagccgggt tgcgggggtc cctgcagcca cccgtttgct tcgaccgccc
      961 tcgccttttc tccagttgtc ttttgggttc aatccatgta acggcctagc cccaaataca
     1021 gtctttttcc ccacaaaata cactcacggc cctatttaca tcctgaatca ttaccaatat
     1081 tcataaagat atatcgtaac aaactggagg acttgaccgg tgtccggccg atggggggat
     1141 acgcagagag ccgggccata tttttcccgg gccaaaggcc cggccaggag gaaagataaa
     1201 aatgaagact gcgataggtc tcctcattaa aatgggtgtg gttatccttt cggttacggg
     1261 ttttctcacg cctttggcca agggtgccga tccacccgtt gctctcaccc cggtgatttc
     1321 cggcctgacc cagccggtgc atatcaccca tgcgggcgat ggcagcggga gattgttcct
     1381 ggtacaacaa accgggcaaa tcaaaatcta tcaaaatggc gccttggcgc ctgtcccctt
     1441 tttggatatc agtagtcgca taagctgctg cggggaacag gggcttctca gcattgcctt
     1501 ccctccccag tatgcccaaa agaaatattt ctacgtatcc tacacgaata ccagcggcaa
     1561 ttcggtgatc tcgcgctttt ctttaacagt caatccggat gtggctgacc cggcgacaga
     1621 gcagatcctg cttacaatca ctcagccctt ttccaatcac aacggcgggc tgatggcctt
     1681 tggcccggac ggatttctgt acattggctt tggggatggc gggtcgggag gagaccccaa
     1741 caactttgct cagaatctca ctgatttgcc cggcaaccag aagctactgg gaaaaatgct
     1801 gaggatcgat gtggaatccg ggactcagcc ctatgccatt cctcccacca atccgctatt
     1861 gaacggggtt cgtagcgaaa tatgggccct gggcctccgc aatccctggc ggttttcttt
     1921 tgaccgacaa acgggagacc tgtacatcgg cgacgtaggc caggcctctt ttgaagaaat
     1981 cgattttcaa ccggcggcaa gccccggggg gcagaattac ggctggcggt taatggaggg
     2041 aaatcattgc ttcaaccccc ctacaaactg caacgacggc accttgaccc ttcccgttca
     2101 tgaatacgac cacacccaag gcgattgttc cgtgatcggc ggttttgtgt accgggggac
     2161 acgctacgtt tccatgcagg gggtttactt ctatggggat ttgtgctccg ggcggatatg
     2221 ggggttgaga aaccaagggg gaacctggca gaatcagctg ctcatcgaca gctccctgtc
     2281 cattaccacc tttggggagg acgagaacgg ggaaatttat gtcgctgact atgcagccgg
     2341 gatcatctac cggatggtga tcgccggaag gctcggcatt ttcaggcagg gaagttggtt
     2401 tctggattca agtgactatg gcgtgtggga gggatgcgtc ctggattctt gcctgggccc
     2461 attcggcttc ccctccgatg tgcccgtggt gggagattgg actgggaccg gtacatccaa
     2521 gatcgggacc ttcaggaatg ggcaatggtt cctggacctg aacggcaatg gcacctggga
     2581 tggctgcggg actgacgcct gcattgcttc ctttggtctg ccgaccgaca ttccggtgac
     2641 cggcgactgg accggctccg gcacctctaa gatcgggacc ttcaggaatg gggaatggtt
     2701 cctggacctg aacggcaatg gcacctggga tggctgcggg accgacgcct gcattacttc
     2761 ctttggtctg ccgaccgaca ttccggtgac tggcgactgg accggttccg gcaccaccaa
     2821 gatcggggtc ttcaggaacg gtgcgtggtt cctcgacttg aacagcaatg gagtttggga
     2881 tggctgcggg gttgacttat gtattccttc tttcggaggt tttccaggtg accttccgat
     2941 cactggaaag ccaggaagct gaacatggca aacctgccaa ggaagggaga tagaacgatg
     3001 gccggcaatg aaagactgat caagatcttc gagcacgcac ttcaacagga atttacgggg
     3061 agagacttct tcaagagctc ccttcaaagg atgggatggg ggtcggcggt gagcgccttt
     3121 cgacgtctca tccaggaaga agaaaaacat atagaaatga tcgaaaagat tctttcaggc
     3181 ctgaagaaag gatcccccat agacctgtcc cccgcaaaaa gcccggcaga gggaccgttc
     3241 aatcactttg aggaaagggc caagaaagag ttcttcgccc aatgcctgga gggttccatg
     3301 gtccccgagg ttacgatctt caacaccgcc tggcttatcg aaaa
//
LOCUS       DASPRR010000366         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1211950, whole genome shotgun sequence.
ACCESSION   DASPRR010000366 DASPRR010000000
VERSION     DASPRR010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211950"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VEL68_13720"
     CDS             complement(<1..814)
                     /locus_tag="VEL68_13720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ37072.1"
                     /translation="MKIAVVFNKAQRPDTTGVYCLRALSQKHEVSHFEPEEIPDIRPD
                     AFDLFMNIDDGRSYRFRSDLHPSLFWAIDTHVKPERCQSKAKDFDFVFCAQRPGAAAM
                     RKAGIAARWCPLAFDPEIHRKHPLPKILDISFVGNSGRFRWGNFFYDGKEVFRERTRL
                     LSLLKRRFNLFTGKFFFEDMGVVFSLSKIVFNRSVKDDVNMRVFEALGCGSLLVTNQI
                     EHSQDLLFKHGEHFIEYRSPRELVERVAYYLHNPEEREQIASRGREEALRKHT"
     gene            complement(828..1499)
                     /locus_tag="VEL68_13725"
     CDS             complement(828..1499)
                     /locus_tag="VEL68_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXZ37073.1"
                     /translation="MIFSLDDDVSRPSERLISVALDAIGKAREVDLEDLCRRMKNPPY
                     YPNVWPGEHYKLLAGLVLALKPQEVVEVGTSMGLSALAMKKYLPPDSKLFTFDIQDWK
                     AHPDYALRREDFADGRLVQFLDDLSDPDICRKYEDLLKQAQLICIDAAKDGVGERKFL
                     ENFSQVPLREGSFLVLDDIRLWKMLKIWREVSLPKLDLTSFGHWSGTGLVEWTSKRPG
                     QKSCP"
     gene            complement(1612..2724)
                     /locus_tag="VEL68_13730"
     CDS             complement(1612..2724)
                     /locus_tag="VEL68_13730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ37074.1"
                     /translation="MKIAMYNLTTTCRFGGVETFVWETSRELARTGEEVHILGGKGKI
                     LKDIPGVKISLFPFWPRERIPNLGTRFRKMGERCSFGRWAYSHLLREKFDIVHIHKPF
                     DLPWSWAVKRKTDCKVILGSHGTDFFPGDKFFIPAVDRAVCCSQFNASQIQKRYGIVP
                     RVIYNGIDPQIFRPLLPDMNLKRQLEFAEGSSVILFAGRLIGLKGVQVLVRAAVRLPR
                     EDPWKILIIGEGEAKESLKNLCRDLGIQERVLFTGFIPNHELPRYYSFADLAVFPSLA
                     DETFGVAICEAMACGRAVLSTRVGGIPELIMDGESGVLVEPNNEGELGDQIARLLRDP
                     ERRLVLGERALDRVRKLFTWEKVAERLKETYRAACI"
     gene            complement(2721..>2970)
                     /locus_tag="VEL68_13735"
     CDS             complement(2721..>2970)
                     /locus_tag="VEL68_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXZ37075.1"
                     /translation="MHMAIGLGVPLIGLFCPTNVEDTGPLGYDKAVILRKERTCQPCL
                     NRDCRDNFCMKQITVEEVCLAAERILDASLSQRIGGSG"
BASE COUNT          662 a          854 c          773 g          681 t
ORIGIN      
        1 aggtatgctt tcgcagggct tcctccctgc cccgggaggc gatttgctcc ctttcttctg
       61 gattatgaag ataatacgcc accctttcga cgagttcacg gggggaacga tactcgatga
      121 aatgctcccc gtgtttaaag agcaggtctt ggctgtgttc gatctggttg gtcaccagga
      181 gacttccgca gcccagggct tcgaagaccc gcatgttcac atcgtctttc acgctcctat
      241 tgaagacgat cttggagagg ctgaagacca cgcccatgtc ttcaaaaaag aatttgccgg
      301 tgaaaaggtt gaaccgtctc ttcaaaagag agaggagcct ggttctctcc cgaaagacct
      361 ccttcccatc gtagaaaaaa ttcccccagc gaaaccgtcc ggaatttccc acgaaggaaa
      421 tatctagaat ttttggcaga gggtgcttgc ggtgaatctc cgggtcaaag gccaggggac
      481 accagcgggc cgcgattccc gccttcctca tggctgcggc tcccggtcgc tgggcgcaaa
      541 agacgaagtc aaagtccttc gccttgctct ggcatcgttc gggtttgacg tgggtgtcga
      601 tggcccagaa gagagacgga tgaaggtcgg aacggaagcg gtaggaacgc ccgtcgtcga
      661 tgttcatgaa taagtcgaat gcatccggac gaatgtcagg aatctcctcc ggctcaaaat
      721 gagagacttc gtgcttctgg ctaagggccc gaaggcaata aactcccgtt gtgtccggtc
      781 tttgggcttt attgaaaacg acggcgattt tcatctatcc ttcttcttca cgggcaggat
      841 ttttggccgg gacgctttga cgtccattcc acgagtccag ttcccgacca atggccgaaa
      901 gaggtcaggt ccagcttggg cagggagact tccctccata ttttgagcat tttccagagg
      961 cggatgtcgt ccagcaccag gaacgaccct tcccgcaggg ggacctggct aaaattctcc
     1021 aggaacttcc tttcgccgac cccgtctttg gccgcatcga tgcatatcaa ttgggcctgt
     1081 ttaaggaggt cctcatattt ccgacaaatg tcgggatcgc tcaggtcgtc cagaaattga
     1141 accagccggc cgtcggcaaa atcttctctt cttaaggcat agtccgggtg ggctttccaa
     1201 tcctggatgt cgaaagtgaa gagcttcgag tcaggaggca ggtatttctt catggccagg
     1261 gcggacaacc ccatgctcgt cccgacctcg acgacctcct ggggcttcag ggcaagcacc
     1321 agacccgcca gtagcttgta atgttccccc ggccatacgt tcggataata ggggggattc
     1381 ttcattctcc ggcacaggtc ttccaggtcc acttcccggg ccttgccgat cgcatccagg
     1441 gcgacagaaa tcaggcgttc ggagggccgg gaaacgtcat catccaggga aaaaatcatc
     1501 gaatactcgg aatgccgaga ctgaaagggg ggcggcggga ttttccgaat atctttacga
     1561 aaccattgca taccgatttt ctcgtttgat caatgaaaat agactaggat atcaaatgca
     1621 agccgccctg tatgtctcct tcagacgctc ggctaccttt tcccaggtga atagctttct
     1681 cactcggtcc agggctctct cccccaacac cagacgcctt tccggatccc gaagaagtcg
     1741 agcgatctga tctcccagct ctccctcgtt gtttggctcc accaggaccc ccgattcccc
     1801 gtccatgatc aactccggaa tcccccccac ccgggtggat aggaccgccc ggccgcaagc
     1861 catggcttca caaatggcca ctccgaaggt ttcatcagca aggctgggga aaaccgccaa
     1921 atcggcaaaa gaataatacc tcgggagttc gtggttggga atgaatccgg taaagagcac
     1981 cctttcctgg attcccaggt cccggcacaa attcttaaga ctttctttgg cctctccctc
     2041 gccgatgata aggatcttcc aggggtcttc ccggggcaag cggactgcgg ctctcacgag
     2101 cacctgaacc cctttcaatc caatgagccg cccggcgaag aggatcaccg aacttccttc
     2161 ggcgaattcc agctgtctct tgaggttcat gtccggaaga aggggacgaa agatttgcgg
     2221 gtcgattccg ttgtagatta cccgcggtac gattccgtat ctcttctgga tttggctcgc
     2281 attgaattgg ctgcagcaca ccgcccgatc taccgccggg ataaagaact tatcccccgg
     2341 gaaaaaatcc gttccgtggc ttcccaggat caccttgcag tcggttttcc ttttcaccgc
     2401 ccaagaccag gggagatcaa agggcttatg aatgtgaaca atatcgaatt tctcccgcag
     2461 caaatgggag taagcccagc ggccaaagga gcatctttcc cccattttgc ggaagcgcgt
     2521 gcccaggttg gggatccgtt ccctgggcca gaaagggaac agggagattt ttaccccggg
     2581 gatatctttc aaaatttttc cctttccccc gaggatatgg acctcctcac ccgtcctggc
     2641 cagctcccgg gaggtttccc agacgaaggt ctccaccccc ccaaagcggc aggtggtggt
     2701 caggttgtac atggcaatct tcaaccggat cccccgattc tttgcgataa agaggcatcc
     2761 aggattcgtt ccgccgccaa acagacttcc tccacggtga tctgcttcat gcaaaaattg
     2821 tccctacagt cccggttcag acagggctgg caggttcttt cctttctcag gatcaccgcc
     2881 ttatcgtagc ccagaggacc ggtatcctcc acgttggtgg ggcagaaaag cccaatcagg
     2941 ggaactccca ggccgatggc catgtgcata
//
LOCUS       DASPRR010000367         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_402359, whole genome shotgun sequence.
ACCESSION   DASPRR010000367 DASPRR010000000
VERSION     DASPRR010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402359"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..614)
                     /locus_tag="VEL68_13740"
     CDS             complement(132..614)
                     /locus_tag="VEL68_13740"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXZ37076.1"
                     /translation="MKKKWWILLGSLVIIAAIGFTEVSHAGRHFSNPMGLGHMYRLNL
                     LSEELGLTDDQRAALKDLIKDHRNEIKLLVKTLIAKKRALQELALGDNPDPAAIRQAS
                     TDLGNAIAEAAVLGSSLAQKAKSILTPEQLTRLREMRQNREKAFDESLREWQERNPAF
                     "
     gene            complement(894..2513)
                     /locus_tag="VEL68_13745"
     CDS             complement(894..2513)
                     /locus_tag="VEL68_13745"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXZ37077.1"
                     /translation="MKVSLGKAIIPLFILGFLTAIGSSASAQDSYDIVIAGGRIVDGT
                     GGPWYYGDVGIQNGRIVRIGDLKEVRAAKRIEAKGLVVSPGFIDIHTHTDEDIEKLPL
                     ARNYMQQGVTSIIGGNCGDSIYPIGEKLAGLERLGLGINFGLLVGQATIRKQVMAMAD
                     RAPTSDELRRMKSLLAKAMEEGAVGLSTGLYYAPGSFCKTEEIIELARVTAQYGGIYT
                     SHIRDESDYSIGLVAAVKEAIEIGEKGKIPVQISHLKALGKPVWGKSVEILDLIRQAR
                     TRGVDVTFDQYPYVASATSLVGATVPRWAQAGGKEKMEERFRDPSARERLRKDMLTSI
                     DKRGGPEKLFIARFTPDPSLEGKHLAAIGKIKSKEPVDAAMDLILAGGLDVSVVSFNM
                     LDEDLTRIMLSPHGLVASDGSLAELGKGVPHPRYYGTFPRVLGKYVREEAILPLEEAV
                     RKMTSAPANRLGLVDRGLIKEGMLADITLFNALTVKDKSTFDNPHQYPEGIDFVIVNG
                     QVAVAKGEWTGLKAGKVLYRQKSFTSARTMP"
     gene            complement(2596..>3296)
                     /locus_tag="VEL68_13750"
     CDS             complement(2596..>3296)
                     /locus_tag="VEL68_13750"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ37078.1"
                     /translation="HNPSESGIPCIDDPEEFRQGVRSAAAQGVTVSLHAIGDAAIGFA
                     EELYWECIQQTIGDAPLRIEHFQHPGEKHIASMNHPRLFASVQPAHLLDDASIAEERL
                     GPRRASLSYPVRSLLERGCRVIFGSDWDVADLNPLVGLQAAVTRVDKANRFPGGWHPI
                     QRISIAQAIECYTANPTLATRLPQRAGKLMEGLEANLTVLDRDITSIPPDQITKAKAL
                     MTIVAGQIVYDESL"
BASE COUNT          683 a          936 c          922 g          755 t
ORIGIN      
        1 ccaggctgtc ggcaatatcc tgttggtctt cctcggaaac catttccgcc ggatccattc
       61 cgcccccgca tatgttcacc aatggcccgc ccgtggtcaa tataacataa agccaagggg
      121 cggattgaag attaaaacgc gggattcctc tcctgccatt cccgcagcga ttcgtcaaag
      181 gctttctcgc gattctgcct catttcccgc aagcgggtta actgctcggg cgtcagaatc
      241 gatttggcct tctgcgccag cgaagatccc aggaccgccg cctcggcaat cgcgttgccc
      301 aagtcggttg aggcctggcg aatcgctgcc ggatccggat tatcccccag cgcaagttcc
      361 tggagggctc gctttttggc tatcaaggtt ttcacaagca gcttaatttc atttcgatga
      421 tccttgatca aatccttgag ggctgcccgc tgatcatccg tgagccccag ttcctcgctc
      481 aaaagattta accggtacat atgaccaaga cccatcgggt ttgagaaatg acggccggca
      541 tgagagacct cggtaaaacc gatggctgca atgatgacca ggcttcctaa aagaatccac
      601 cattttttct tcatcggaac ctccttataa tgattgattc ccaccctttt ggttcgagtg
      661 ggtttttctc ctacaaccga atagtggatt ccgaatcgaa aaaggttaca tcgcccggaa
      721 taatttgggc aagtatcgcc cgaagttagg agtccgaaag gccacctggg gagaagaagg
      781 ggccagtata ggaagactgg atcgggcgaa accagaagaa aagcggaggc agaatatatg
      841 agaagaaaag ggagtagggg cggttcgcca accgcccctg cattcaatcg gttctaaggc
      901 atggttctgg cggaggtaaa ggatttttgg cggtaaagaa cctttccagc ttttaagccg
      961 gtccattcgc ccttggctac ggcaacctgc ccgttcacga tgacgaagtc gatgccctcg
     1021 ggatactggt gcggattgtc aaaagtggat ttatctttga ccgtgagagc attgaaaagg
     1081 gtgatgtcgg cgagcatccc ctccttaatg aggccccggt ccaccagccc taggcgattg
     1141 gccggagctg aagtcatttt gcggactgct tcttcaagag gaagaatggc ctcttctctc
     1201 acgtatttgc ccagaactct cgggaaggtg ccgtaatacc ggggatgggg gactcctttc
     1261 cctaactccg caaggctccc gtcggaggcc acgaggccat ggggagaaag cataatccgg
     1321 gtcaggtcct cgtcgagcat gttgaaggaa accacgctga cgtcgagtcc accggccagg
     1381 atgaggtcca tggccgcatc cacgggttcc ttgcttttaa tctttccgat cgcggccaga
     1441 tgttttccct ccaggcttgg gtcgggagtg aagcgggcaa tgaagagctt ctccggccca
     1501 ccccgtttat cgatcgaggt caacatgtct ttcctgagcc tctccctggc cgacgggtcc
     1561 cgaaagcgtt cctccatttt ctctttgccg ccggcctggg cccaccgggg aaccgtggcc
     1621 cccaccagac tggtggcaga ggccacatag gggtactggt cgaaggtcac atcgaccccc
     1681 cgggtgcggg cctgccggat caggtccagg atttccaccg attttcccca gaccggcttt
     1741 cccaaagcct tgaggtggga aatctggacg ggaatcttcc ccttttctcc gatctcgatg
     1801 gcttccttca cggcggcgac cagcccgatg ctataatcgc tttcatcccg gatatggctc
     1861 gtatagatgc cgccgtactg ggccgtgacc cgggccagct caatgatttc ctccgtcttg
     1921 cagaaggagc cgggggcata atacagaccg gtagaaaggc cgactgctcc ctcctccatt
     1981 gcttttgcca gcaggctctt catccttctt aattcgtcgg aggtcggggc ccggtcggcc
     2041 atggccatga cctgtttgcg aatggtcgcc tgtccgacca ggagaccgaa gttgataccc
     2101 agccccaatc gttccagacc ggctagtttt tctcctatgg ggtagatcga gtccccgcaa
     2161 ttccccccga tgatgctggt caccccttgc tgcatgtagt ttcgggccag ggggagtttt
     2221 tcgatgtcct cgtcggtgtg ggtatgaata tcgatgaacc ctggggaaac caccagacct
     2281 ttggcctcga tccgcttggc tgctcgaacc tctttcaggt ctccgattct aacgatgcgt
     2341 ccgttctgga tgccaacatc cccgtagtac caagggcctc cggtcccatc cacaatccgg
     2401 ccccccgcaa tcacgatgtc gtaagagtcc tgggccgaag ctgaagaacc aatagctgtg
     2461 aggaagccaa ggatgaagag gggaattatt gccttgccga gtgaaacctt catgggatct
     2521 cctcctttca gtggctgggt gctcttgcat gatagggcga ttcatgtcgg agactcgact
     2581 ctacgagaat cgcccctaca gactttcgtc atagacgatt tgcccagcaa caattgtcat
     2641 cagtgctttg gccttcgtaa tctggtcggg gggaatcgag gtgatatccc ggtccaggac
     2701 cgtgaggttg gcctccagtc cttccatgag ttttccggcc cgctggggga ggcgagtcgc
     2761 aagagtgggg ttagcagtgt aacactcaat cgcctgggcg atgctgatcc gttgaatcgg
     2821 atgccaacca ccggggaaac gattggcttt gtccactcgg gtcactgccg cttgaagacc
     2881 taccagggga ttgaggtcgg ctacgtccca gtccgagcca aagatcaccc ggcaccctcg
     2941 ttccagaagg gatcgaacgg ggtaggagag agacgctctc cggggtccca gtctttcttc
     3001 ggcaatggaa gcatcatcca ggagatgggc cggctgaact gaagcaaaga gccggggatg
     3061 attcatggag gcgatatgct tttcccccgg gtgttgaaaa tgctcgatgc gcaggggagc
     3121 gtcccctatt gtctgctgga tgcactccca gtacaactcc tctgcaaatc cgatggccgc
     3181 gtcgccgatg gcgtggaggg acaccgtaac tccctgcgcg gcagcgcttc tcaccccttg
     3241 ccggaattct tcggggtcgt cgatgcaggg gattccgctc tccgagggat tgtgga
//
LOCUS       DASPRR010000368        10769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1376541, whole genome shotgun sequence.
ACCESSION   DASPRR010000368 DASPRR010000000
VERSION     DASPRR010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376541"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..792
                     /locus_tag="VEL68_13755"
     CDS             94..792
                     /locus_tag="VEL68_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ37079.1"
                     /translation="MLLHVRDLETGYQNKQVLFGVSLDLEEREIVALLGHNGAGKSTL
                     VSAIYGLLPLYRGRIEFQGQNIAGKKILSNVQKGLVLVPGGHQVFGELSVMENLKVVQ
                     YLHKKENFASQLAEIFRIFPWLSERKSQTAHTLSGGEQQMLSIALGLLMKPKLLMLDE
                     PSLGLAPLMVANIMEAIQLINREYHVGILLVEQNIRYALETSSRVYVMRLGKMITQSR
                     TDELRQKENYVDLF"
     gene            complement(1074..1949)
                     /locus_tag="VEL68_13760"
     CDS             complement(1074..1949)
                     /locus_tag="VEL68_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXZ37080.1"
                     /translation="MISIDKNLCNNCGICVAICPRRILRLGEESAEVTDQALCMVCGH
                     CKAVCPKNAPGFTMGDEDFEPLSPKEEILSPSDFLHFIRRRRSIRQYKEEPIEKEKLA
                     MTIQAGRYSPSGGNRQACEFTVISGRKTLDEVCTLAIQQLLNEARVIRNIFDRHERFK
                     EPIPEEYIARKVLPSVWERIGRAWEGGQDQLLFHAPSLIVIHLNEGSATTGVIDAGIA
                     SMNMIYMAETLGLGACYIAFLVWAVQGSKALQTFLKIPAGNRLHIAFTVGYPKVKYLR
                     LVARRPAKITWLGDS"
     gene            2241..3125
                     /locus_tag="VEL68_13765"
     CDS             2241..3125
                     /locus_tag="VEL68_13765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37081.1"
                     /translation="MPSDLRMTAEILQQIVNGLIIGATYGLMAVGLTMIFGMMDIINF
                     AHGELYMLGGVFAYYSVSLFSLNYFESMALSVVLVMVVGLIFDQLVLRKLRDEPTLIT
                     ILVTIGLSIFLQSTVMLFWGPSPQGIPSPFTTKTIQIGAIFLTPFKVFMAAVTVAVIF
                     GLHLMIQKTTLGKTMRAVFQEKEAAAIVGININRVYAINFALGSGLAALAGSLLGSLF
                     VVYPNMGLIAVVKAFLIVIMGGMGNFLGAIFGGLLLGVVESMAAGFISSSYKDLFGLL
                     IVMILLVVRPYGLFGKKI"
     gene            3134..4102
                     /locus_tag="VEL68_13770"
     CDS             3134..4102
                     /locus_tag="VEL68_13770"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37082.1"
                     /translation="MGRKNQTIGLLLLVIVALLAPFFIRDEYFIHLLINAGLFYILVA
                     GLNLLVGYLGKLTLGHTAFLGIGAYAAALLNLKLQAPFVVSLAGSVVISFVLAFLFGL
                     IVLRLRGPYFVIVSLCFAEMLAIVATNWVSLTNGPMGLPGVEPPKFSIPDLFEFYLDT
                     KVRFYYLMIALVIIVTYITHRLVHSVIGRASISLRENENLAESVGVSAFRYGMIVFTV
                     ASVFAGVAGCFYAHYVSYLNPEIFGFSYMITMLVMLVTGGKGTIAGPFYGCILFTVLP
                     EALRVAKDYRDPVFGITLIIVAIFLPQGIAGLFGRLWARFKGREVA"
     gene            4106..4873
                     /locus_tag="VEL68_13775"
     CDS             4106..4873
                     /locus_tag="VEL68_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37083.1"
                     /translation="MYLTVENLSIAFGGLRAVDELSFHLNQGEILSLIGPNGAGKTTT
                     FNLITGFLNPNQGRIVFKGKEISRLTPNKIARHGITRTFQKTNIFPGVAVMDCVVMGG
                     YRRVKESLVDILFKTRTFDRAEKALQEQAEAILQFLGLEKRKGSLGRNLSYGEQRLLE
                     IAIALAAEPELLLLDEPVAGMNPSESEQVMTVVAEIRKRGMTILLVEHNMDVVMNISD
                     RIVVLDHGRKIAEGLPSQVQEDPQVLEAYLGKGYSRA"
     gene            4866..5600
                     /locus_tag="VEL68_13780"
     CDS             4866..5600
                     /locus_tag="VEL68_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37084.1"
                     /translation="MLEAQEIHSAYGKVRALFGVSLHVREKELIALIGANGAGKSTTL
                     RTIMGLIPPASGQIHFMGKRIDGLPARAIVKMGISLCPEGRKLWPEMTVRENLELGAF
                     SRGDREGARKDLERVFDSFSILRERQDQMAGSLSGGEQQMAAIGRALMSSPRLLMLDE
                     PSLGLSPIIMEKMSGMIHQIHQSGTTILLVEQNAFLALQMSNRGYVLEVGRVAIVGNS
                     SDLMENEHVRKAYLGRKVGEKPAATR"
     gene            5624..6388
                     /locus_tag="VEL68_13785"
     CDS             5624..6388
                     /locus_tag="VEL68_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXZ37085.1"
                     /translation="MAQKTIQSLEKGLDILFLFSEERPFLTLEEISSRSGLPKSTCYR
                     FLSTLKNKNILERERTSGKFKLGVRLLKLESVIHNSLGIARIASPFLQKLSEVSGETA
                     QLVVLNKNEGVCVERVESTATLRVMPDRGTTIYLHSGASGKVILAYLNPEERSRIIHE
                     KGLKRLTPNTITAPSALKRELEEIRKRGYAVSDQEIYQGVKAIAAPIFDFQGKITASL
                     GVAGPRERLTGKKINSLAPIIKEAARSISEQLGADS"
     gene            6410..7192
                     /locus_tag="VEL68_13790"
     CDS             6410..7192
                     /locus_tag="VEL68_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HXZ37086.1"
                     /translation="MGILQNLSWDEAAKELKTTHVALIPVGSTEQHGYHMPLGSDTYC
                     ADAVARRTATKESAIVVPAIPYGISHSHMSFPGTITFSCETLFRAVTDVCECLWKHGV
                     NRFILVNGHGHNGPTLQTVMDEFKRNHKAYLFLIQWWIAGYRLTPDLWTSKKDELPDG
                     HAAEVEAAGMLAIDSTLVKMGKAEKVLLGTLGKSKIKFHKSTMAALKDYPVDLTTVED
                     FEHLSKSGVVGSPLGATKEKGEIVLDKVTDFLADLVRELKKI"
     gene            7309..7689
                     /locus_tag="VEL68_13795"
     CDS             7309..7689
                     /locus_tag="VEL68_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HXZ37087.1"
                     /translation="MKKEIIRTDKGFVSKSPLSQAVKVGSNVYISGQVPVDSKTMLVV
                     SDDFAVQARHVLESIKGIVEAAGGSMQSIIKTTVFLTDMGRFQEMNEIYKTYFPEEPP
                     ARTCIGVKELPRKSQIEIEAVAML"
     gene            7764..8909
                     /locus_tag="VEL68_13800"
     CDS             7764..8909
                     /locus_tag="VEL68_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37088.1"
                     /translation="MALFGCCWGILIAMLAISPALGQDKKPLAIGAVQPLTGWAALGG
                     KNVVAGMKIALEKVNASGGVLGRPLELIIEDGKADPVESVNAVEKLIRRDKVPAIIGC
                     WASSATLAAMPIMERSKIPMLVETATAPMITDKKNKWVFRFTSNNDIDGLLMEPYLVP
                     KLGFKKAAYLAVNNDWGRSMVKAVSEIMKKTGGEVVMVEYCGGAEANFTPMLTRIKNA
                     GADTMFITNTLNGIALILKQYHELGMKMNLFITSGMSVEELVNLAGKEAVEGSYFFER
                     FVASSPPPGKEKESQEMIAAYKKIFTDGFAYADVAQGFDSVQIMAQAIQRAGTPDPPR
                     IREALEKTDYPGVSGRVKFDQNNHSHPRCSITQARNGVNTVVYILKE"
     gene            9018..10172
                     /locus_tag="VEL68_13805"
     CDS             9018..10172
                     /locus_tag="VEL68_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37089.1"
                     /translation="MKGNFWRWILVAGFIGFLLAGPGTAQDKSPIVIGAVQPQTGPVS
                     IDGMSVVSGIKIATERINAEGGVLGRPIQVVIEDGKGDPVESVNAVEKLIRRDKVPAI
                     IGCWASSATLAAMPIVKRNQVPLLVETSTAPMITDQKNEWVFRFTSHNDIDGALMEQY
                     LVPKLGFKKAAYLAVNNDWGRSMAAAVGKIMEKTGGKVLFVEYASAAETNYTPMLTRI
                     KASEADTLFITTSIQSVAMILKQFRELGMKMNVFVTSAIPAEKVIDIAGKEASDGVYF
                     FNRYVAYAPPRGKEEENKKLVEAYQKIYPGKAPDMYVAFGYDALYIMADAIKRAGEPS
                     ASKIREALETTDFFGLTGRVKFDANHHSQPRVSISQVKNGSPQIVYQLKD"
BASE COUNT         2510 a         2756 c         2993 g         2510 t
ORIGIN      
        1 tgaagggagg atcgtagcct gcggaaatcc cctggagttg cgtcaggatg aaaagctcca
       61 gcgggcgttc ttttacgggg cctgattcct gccatgctat tgcacgtccg cgacctagaa
      121 accggttatc agaataagca agtccttttc ggagtttctc tggatttgga ggagcgggaa
      181 attgtcgccc tcctgggtca caacggcgcc gggaaaagca ctttggtcag cgccatttat
      241 ggccttcttc cgctttatcg cgggcggatc gaatttcagg gacagaatat tgccggaaag
      301 aagattttaa gcaacgtgca aaaaggactg gtcctggttc cgggagggca tcaggttttt
      361 ggtgaactat cggtgatgga gaacctgaag gtcgtccaat accttcataa gaaagaaaat
      421 ttcgcttccc aactcgcgga aatattccgg atctttccct ggctctctga aagaaaatca
      481 cagacggccc acacgctgag cggcggtgag caacagatgc tatccatcgc cctgggattg
      541 ctcatgaaac ccaagctcct catgctggat gagccatccc tgggactggc gccccttatg
      601 gtcgccaata tcatggaagc gatccaattg attaacaggg aataccatgt aggcatcctc
      661 ctggttgaac agaatatccg gtatgccctg gagacatcat cccgggttta tgtaatgcgc
      721 cttggtaaga tgattacgca aagccgaact gacgagctgc gccaaaaaga aaactatgtt
      781 gacctttttt agatcggatt gcaggaagat cccgaaggaa atcatccgta gacccgcgat
      841 caagaattaa ccggatgcgg aaaaactcca attccccgtc cctcccgcga aggcgggagt
      901 ccagaaatcc ttgaagaaac tggggcatgg cgttaatgat tcatagcagc ggagattcgc
      961 tgatgcccgc tgcggcgtgt ggccactttt cgggagacat gatgcccgcg aaagaagggc
     1021 aggaattctg aactccgatg cccggggtcc tgagtggctc accctttttc ctttcacgag
     1081 tccccaagcc aggttatttt tgctggcctg cgggccacca ggcgcagata ttttactttt
     1141 ggatatccca cggtaaaggc gatatggagc cggttgccgg ctgggatttt caggaaggtt
     1201 tgaagagctt tggaaccttg aaccgcccat acgagaaaag cgatatagca ggcccctaat
     1261 cccagggttt cggccatgta aatcatattc atggacgcga ttcccgcatc gataacgccg
     1321 gttgtggccg atccttcatt aagatgaatc acgattaagc tcggagcgtg aaataaaagc
     1381 tgatcctgcc cgccttccca ggctcgaccg atgcgttccc acacggaagg aagaactttc
     1441 ctggcaatat attcctcggg gatgggttcc ttaaagcgct cgtgccgatc aaaaatattc
     1501 cggataaccc gcgcttcgtt gaggagctgc tggattgcca gcgtgcagac ttcgtcgagg
     1561 gtttttctcc cgcttatcac cgtaaattcg caggcttgtc ggtttcctcc cgaaggggag
     1621 tagcgtccgg cctggatagt catggcgagc ttttctttct caataggttc ttccttatac
     1681 tgacgaatgc tccgacgtcg ccggatgaaa tgaagaaagt ctgacgggct tagaatctct
     1741 tcctttgggg ataggggctc gaaatcttca tcgcccatag tgaatcctgg cgcattcttt
     1801 gggcagacag ctttacaatg gccgcagacc atgcacaagg cctggtcggt tacttcggcg
     1861 ctttcctccc cgaggcgcag gatcctccgg ggacagatcg caacgcatat tccgcaattg
     1921 ttacataggt tcttatcaat cgagatcatc agaccgtccc tccttttagc ttttcttacc
     1981 acgagccatt acctgcgggc aatgagcgaa ttctcttaat gtccttgaaa ggggccctag
     2041 gcgcgctccc tcctccttga tccagacccc ttacccttga accttatacc ctgcaatatt
     2101 cacctttcat gctttaagcc tttcatcttc tttaagaatg ttgccagcca ttttggtctt
     2161 gacaaaattc ggccaattga atagatatca taaagagaat tgacagtctc ataatgtaag
     2221 aaaacgaagg gaaggactta ttgccctcgg acctgcggat gacggcggag atcctccagc
     2281 aaatcgtaaa cggactgatc atcggggcaa cctacggcct catggccgta gggctgacga
     2341 tgatcttcgg catgatggat atcattaatt tcgcccacgg tgagctctat atgctgggag
     2401 gggtgttcgc ctattattcg gtcagcctct ttagcctgaa ctatttcgaa tccatggccc
     2461 tctccgtggt attggtgatg gtggttgggc ttatttttga tcagctggtt ctccgaaaac
     2521 ttcgtgatga accaaccttg atcaccattt tagtcactat cggcctttcc attttcctcc
     2581 agagtacggt catgctcttc tggggtccct ccccccaggg gattccttcg ccctttacca
     2641 cgaagaccat ccagatcggg gcgatctttc tcaccccctt caaggttttt atggctgcgg
     2701 tgacggtggc ggttatcttc gggcttcatc tcatgatcca gaagacgacc ctgggcaaaa
     2761 ccatgcgcgc cgttttccag gagaaggaag cagcggccat tgtcgggatc aatatcaacc
     2821 gggtctatgc catcaatttc gccctggggt ccgggttggc cgctcttgcc ggctctctcc
     2881 tgggctctct ctttgtggtt tatcccaata tgggcctcat cgccgtggtc aaggcgttcc
     2941 tgatcgtgat catgggcggg atgggcaact tcctgggggc gatcttcggt ggactccttc
     3001 tgggggtggt ggagagcatg gccgcaggat tcatttcctc cagttataag gatctcttcg
     3061 gtcttctcat cgtcatgatt cttctcgtgg tccggccgta cggcctgttt ggaaagaaga
     3121 tctagggggg ggagtgggga gaaagaatca aaccatcggc cttctgctgt tggtgatcgt
     3181 ggccctccta gctccttttt tcatccggga tgaatatttc atccacctcc tcatcaatgc
     3241 cggccttttc tatattttgg tcgctggcct gaacctcctc gtcggttacc tcgggaagct
     3301 taccttgggg catacggcct ttttggggat cggggcctac gccgcggccc ttctcaattt
     3361 gaaactccag gctccctttg tggtcagcct ggccggctct gtggtcatca gttttgtcct
     3421 ggcctttttg ttcggcctga tcgtgctccg attgcggggc ccttacttcg tgatcgtgtc
     3481 cctgtgtttt gccgagatgt tggcgattgt ggccacgaac tgggtcagcc tcaccaatgg
     3541 ccccatgggg cttcccgggg tggaacctcc gaaattttcc atccccgacc tgtttgagtt
     3601 ctatttggat acgaaggtca gattctatta cctgatgatc gccttggtga tcatcgtgac
     3661 ctacattacc caccggctcg tccattcggt catcggccgg gcctcgatct ccctgcggga
     3721 gaacgagaac ctggcggaat cggtgggcgt cagcgctttt cggtatggca tgattgtttt
     3781 tacggtggct tcggtattcg cgggagtggc gggctgtttc tatgcccatt atgtgagcta
     3841 cctcaacccc gaaatttttg ggttcagcta tatgatcacc atgctggtga tgctggtcac
     3901 cggtggaaag ggtaccatcg ccgggccctt ttacggttgt attctgttca ccgtcctccc
     3961 cgaagcccta cgagtggcca aggattaccg tgacccggtc tttggcatca ccttgatcat
     4021 cgtcgccatt ttccttccgc aggggattgc aggtttgttc ggaaggcttt gggcccgatt
     4081 caaagggagg gaggtcgcct aggtgatgta ccttacggtt gaaaacctct cgattgcctt
     4141 cggaggcctc cgggccgtgg atgaactgag ctttcacttg aaccagggtg aaatcctcag
     4201 cttgatcgga cccaatggtg ccggaaagac cacgaccttc aacctgatca ccggtttcct
     4261 gaaccccaac cagggaagaa tcgttttcaa aggaaaggaa atcagccgtc tgactccgaa
     4321 caagatcgcc cggcatggaa tcacccggac tttccagaag acgaatatct tccccggtgt
     4381 agccgtcatg gattgtgttg tcatgggggg ctaccgacgc gtgaaggaat ccttggtgga
     4441 tatcctcttc aaaacccgaa cgttcgatcg agccgaaaaa gccctccagg aacaggctga
     4501 agccatcctc caattcctgg gtctggagaa aaggaaagga agcctgggtc gaaacctttc
     4561 ctatggagaa cagagactct tggaaatcgc catcgcgctg gccgccgagc ccgaactgct
     4621 ccttctcgat gaacctgtgg ccggcatgaa ccccagcgaa tcggagcaag tgatgacggt
     4681 ggtcgccgaa atccgcaagc ggggtatgac catcctgctg gtggaacata acatggacgt
     4741 ggtgatgaat atttccgacc ggattgtggt tctcgatcat ggacggaaga ttgccgaagg
     4801 tttgccgagt caagtccagg aagacccgca ggttctggaa gcctacctgg ggaaggggta
     4861 tagccgtgct tgaggcgcaa gagatccata gcgcctatgg caaagtgaga gcgctttttg
     4921 gcgtttccct gcatgtgcgg gaaaaggaat tgatcgccct catcggagcc aatggcgcag
     4981 gcaagagcac caccctgcgg acgatcatgg ggctgattcc ccccgcttca ggtcagattc
     5041 atttcatggg gaagcgaatc gatgggctgc cggcccgggc gatcgtgaag atgggcattt
     5101 ccctttgccc ggagggaaga aagctatggc ccgagatgac cgtgagggaa aacctggagc
     5161 taggcgcctt cagccggggg gaccgggagg gggcccgaaa ggacctggag cgggtttttg
     5221 attccttttc tattctgcgg gagcgacagg accagatggc cggcagtctg agtggagggg
     5281 agcagcaaat ggcggccatc ggccgcgcct tgatgtcaag cccccgattg ctcatgctcg
     5341 atgaaccttc cctcggccta tcccccatta ttatggagaa gatgagcggg atgattcatc
     5401 agattcatca aagcgggacc acgatccttc tggtcgaaca aaatgccttc ctggctctcc
     5461 agatgtccaa ccggggctac gttctggagg tcggacgggt agccatcgtg gggaactcct
     5521 cggacctgat ggaaaacgaa catgtccgca aagcctacct gggaaggaaa gtaggggaaa
     5581 aacccgctgc gacaaggtag accaattctt tcgaggaaga tctgtggcgc aaaaaacgat
     5641 tcaatctctg gaaaaaggcc tggatatcct ttttctattc agcgaggaaa ggcctttcct
     5701 taccctggag gaaatctcat cccgaagtgg cctgcccaaa agcacatgct accgcttttt
     5761 gagcaccctc aagaacaaaa acatcctgga aagggagcgg acctcgggga aattcaaact
     5821 tggggtgcgt ctgttgaagc tggaatcggt gatccacaat tccctgggca tcgcccggat
     5881 cgcttcaccc tttctgcaaa agctctccga ggtctccgga gaaacggcgc agttagtggt
     5941 tctgaataag aacgaaggag tctgcgtgga acgggtggag agcactgcga ccctgagggt
     6001 aatgcccgac cgcgggacaa cgatctatct ccacagcggg gcatccggga aagtgatcct
     6061 ggcctatttg aatccggagg agaggtcccg aatcatccac gagaaggggc tcaaaaggct
     6121 cacccccaat accatcaccg ctccttccgc cctaaaaaga gaattggaag agatccgcaa
     6181 gcggggctat gcggtgagcg accaggaaat ttatcaagga gtgaaggcca tcgccgctcc
     6241 catcttcgat tttcaaggga agattacggc cagcctgggt gtggcgggtc caagggaacg
     6301 tttaacggga aagaagatca attccttggc tcccatcatc aaagaggctg cgcggagtat
     6361 ctccgagcaa ctgggagccg attcttgatc gaaggagaaa ggaggaacta tgggcatcct
     6421 acagaatcta tcttgggatg aagccgcgaa ggaattgaaa acgacccatg tggcactgat
     6481 cccggtgggg agcaccgaac agcacggcta tcacatgccc ctggggtccg atacctattg
     6541 cgccgatgcg gtagcaagac gcacggcgac caaagagagt gccattgtgg ttcccgccat
     6601 tccctacggg atctcgcatt cccacatgtc gtttcccggc acaatcacct tctcgtgcga
     6661 gacgctcttc cgggcggtca ccgacgtatg cgaatgcctt tggaagcacg gcgtaaacag
     6721 gttcattctc gtcaatggcc acggccacaa tgggccgacc ctccaaaccg ttatggatga
     6781 gttcaagagg aaccataagg cctatctctt tctcatccaa tggtggattg ccggataccg
     6841 gcttacgcct gacctatgga catccaagaa agacgagctg ccggatgggc atgcggccga
     6901 ggtggaagcc gccggcatgc tggccatcga ttccaccctg gtgaagatgg gcaaggccga
     6961 gaaggtcctt ctgggaacct tgggcaagag caagatcaaa tttcataaat ctaccatggc
     7021 ggccctcaag gattatccgg tagatctcac caccgtagag gatttcgaac acctcagtaa
     7081 gtccggggtg gtcggaagtc ccctgggcgc cacgaaggaa aaaggggaaa ttgttttgga
     7141 caaggtaacg gattttctgg ccgacctggt gcgggaattg aaaaaaatat aataaaggtt
     7201 aaggccaaga tcgagaaaaa gaatcagtga tggaaatttt cttttatctg tttccctcaa
     7261 ctttaacctt gatctgggcc ctacccgagg ccttgattgg gaggagagat gaaaaaagag
     7321 atcatccgga cggataaagg ttttgtttcg aaatcgccgc tttcccaggc cgtaaaagtg
     7381 ggcagtaatg tctacatctc cggtcaggtc cctgtcgatt ccaagaccat gctggtcgtt
     7441 tccgatgatt ttgccgtcca ggcgcgacat gtcctggaaa gcatcaaggg tatcgtagaa
     7501 gcggcgggcg ggtccatgca aagcatcatc aagacaacgg tatttctcac cgatatgggt
     7561 cgcttccaag agatgaatga aatttacaag acttatttcc cggaagagcc tcccgcccgg
     7621 acttgcatcg gggtgaagga gcttcctcgg aaatcgcaga tcgaaatcga agccgttgcc
     7681 atgctgtaac cggcggatgg gctatcccgg cctctgcttt aacttgtgat gaatcatcga
     7741 aaggagagac tacattgcgg aaaatggcac tgttcggttg ttgctgggga atcctcattg
     7801 cgatgctggc gatctcgccc gctctcgggc aggacaagaa acccctggcc attggagcgg
     7861 tccagccctt gaccggttgg gcagccctgg gagggaaaaa tgtcgtggcc gggatgaaga
     7921 tcgccctgga aaaggtcaac gcctcggggg gagtcttagg gcgtcccttg gaattgatca
     7981 ttgaagatgg gaaagccgac ccggtcgaat cggtgaacgc ggtcgaaaag ttgatccgca
     8041 gggataaggt accggccatt atcggctgct gggccagctc cgctaccctg gcggccatgc
     8101 ccatcatgga aagaagcaag attcccatgc tcgtagaaac cgctaccgca cccatgatca
     8161 ccgataagaa gaacaaatgg gttttccggt tcacctcgaa taatgatatc gatggtcttc
     8221 ttatggagcc gtacctcgtc cccaagctgg gcttcaagaa ggccgcgtac ctggcggtca
     8281 acaacgattg gggccgatcc atggtcaagg cggtgtcgga gatcatgaaa aagaccggcg
     8341 gggaagtggt catggtggag tactgcggag gggcggaagc gaatttcacg cccatgctga
     8401 cgcggatcaa gaacgcgggg gcggacacca tgttcatcac gaatactctc aacggcatcg
     8461 ccttgatcct gaaacagtac catgagcttg gcatgaagat gaacttgttc atcaccagcg
     8521 ggatgtcggt cgaggaattg gtcaacctgg ccgggaaaga ggcggtcgaa gggtcctatt
     8581 tcttcgaacg ttttgtcgcc agctcgcctc cgccaggcaa agaaaaagag agccaagaga
     8641 tgatcgcggc ctacaaaaag attttcaccg atggattcgc ctatgccgat gtggcccagg
     8701 gattcgattc ggtacaaatc atggcccaag ccatccagag agccggtacc cccgatcccc
     8761 cccggatccg ggaggccctg gagaagaccg attacccggg ggtcagcggg cgggtcaagt
     8821 ttgaccagaa caaccactcc catccgaggt gttcgatcac ccaggctcga aatggggtga
     8881 ataccgtcgt ctatattctc aaggagtagc cagggggatg gcgaagatgg atgattggag
     8941 gttttctaga agaaggtgct cttaaaatga ccaggacaaa atagctggct ttcaattcag
     9001 aaatgggagg gcttacgatg aaagggaatt tttggaggtg gattctagtc gccggtttca
     9061 ttggttttct attggctggc ccaggaacag cccaggacaa gtcgccgatc gtgatcgggg
     9121 ccgtgcagcc tcaaactggt cctgtttcga ttgatgggat gagtgtggtt tccgggatta
     9181 agatcgccac ggagaggatc aatgcggaag gcggggtcct cggaaggccg atccaggtgg
     9241 tcatcgagga tggaaaagga gatcccgtag aatcggtaaa cgcggtggag aaactgatcc
     9301 ggcgggataa ggttccagcc atcatcggct gctgggccag ctcggcgact ctggcggcca
     9361 tgccgatcgt gaaaaggaat caggtcccgc tgctcgtgga aacttccacg gctcccatga
     9421 tcaccgatca gaagaacgaa tgggtgtttc ggtttacttc ccataacgac attgacggcg
     9481 ctttgatgga acagtatctg gtgcccaagc tgggcttcaa aaaggcggcg tacttggccg
     9541 tgaacaacga ctggggacgc tccatggccg ccgccgtcgg caagatcatg gaaaaaaccg
     9601 gggggaaagt tttgtttgtg gaatacgcct cggcggccga gaccaactat actcccatgt
     9661 taacccggat caaggcctcg gaagctgata ccctgttcat tacgaccagc atccagagtg
     9721 ttgccatgat cctgaagcaa ttccgtgaat tgggcatgaa gatgaacgtt tttgtgacca
     9781 gtgccatccc ggcagagaag gtcatcgata ttgccgggaa ggaggcttcc gacggggtgt
     9841 atttcttcaa tcgctatgtg gcctatgcac cgcccagagg gaaagaagaa gaaaacaaga
     9901 agttggtcga agcctaccag aaaatttacc ccggcaaagc acccgatatg tatgtggcct
     9961 ttggatacga tgccctctat atcatggcgg atgcgatcaa gcgagccgga gagcccagcg
    10021 cgtccaagat ccgggaggct ttggaaacga ccgatttctt cggtctgacg ggccgggtga
    10081 agtttgatgc caaccaccat tcacagcccc gggtttccat atcccaggtg aagaatggtt
    10141 cgccgcagat tgtctaccaa ctcaaagatt aaaggtcgag aaaagggggt actggcaatg
    10201 atttcattcc agtgcccccg attcagttga accacagttc ctagcttgtc attttttctt
    10261 tttcccttta ttacatctcc caattttata ttgtgaaaac ggggtcagta gaaaataggg
    10321 tcaggcttag gtatttaggt attgactagc tgttcttttg tgggtatatg cggttatggc
    10381 gaggaaacca agagttcatt tccagggagc gctttatcat gtgatggtac ggaggaacaa
    10441 gcggcaaaga attttccgga gagaggaaga tttcaaactc tatctgagtg ggccatgtaa
    10501 actccggcac tggtatgaaa tttggcccgg tcaaaaagac gatcgaaggc ctcctggtcc
    10561 gctttgctca aagcccgcct aaatgctttc caccattcct cttccgattc caggacgtgc
    10621 ctaaaaggca taactgtccg gaccattttc ccctgccaga catttatgtg gcaattatca
    10681 ggtcctgccc cagatttttt aataggttca tattactcat catgtaatga gtaatccccg
    10741 tgcacttacg gcatcagtta gagcttgcc
//
LOCUS       DASPRR010000369         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_90041, whole genome shotgun sequence.
ACCESSION   DASPRR010000369 DASPRR010000000
VERSION     DASPRR010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90041"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VEL68_13810"
     CDS             complement(<1..567)
                     /locus_tag="VEL68_13810"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HXZ37090.1"
                     /translation="MTEKKEILYCGDATLATGACYLGGVMTLAGIGFDYVKMEMHFPE
                     RLLQKDYGLIILSDYPSGNFPAGALDKIALKVEQGTSLLMIGGWESFHGLIGNYHTST
                     LAPVLPVECLKEDDRLNWCQGLVPEVVSQHPILKGLPWDQPPLVCGCNRVKEKKEATV
                     VLGLRRLRIDKGKLALDQEFLPFLVTGTY"
     gene            complement(582..1415)
                     /locus_tag="VEL68_13815"
     CDS             complement(582..1415)
                     /locus_tag="VEL68_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ37091.1"
                     /translation="MDPRTRQNLIEFLKHAVMILICAVALSPILWIGTQAFKSYFDTI
                     AVPPKVFFTPVLENFRQVLVKPGFLASIWDSFIMAAGSSILGLLLGTPCGYALARLRY
                     RGSEGVGFFLLITRMVSPIVIIIPLNRAFQFFGLIDTHVGLILAHTFINIGLVVWMMR
                     GFFQDIPDSLEEAARLDGCSNIQAFYRVALPTTAPGLAATTIFCFLFSWNELMMALTL
                     TSLQVRTTPVFIISEFVGYLAVEWGLLSAAGILIVLPMLIFVVAIQKNLVRGLTMGAV
                     K"
     gene            complement(1425..2300)
                     /locus_tag="VEL68_13820"
     CDS             complement(1425..2300)
                     /locus_tag="VEL68_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ37092.1"
                     /translation="MGKLKMDLERGAALILTLPVLLFLVAIFVYPLLYGIYLSFHSTS
                     KGLSNLQYVGLKNYWDILTSQLFWLGMKNTVVYTVGTVGFTVGFGLGIALLLNSIEKG
                     VWIYRAILILPLGMSAVVSGMTWQMMMNPLSGIINYLLQSMGLPTSLWHTGINSAMIS
                     VIIIESWQWTPFPLFVIYAGLQMLPQEPFEAARIDGASAWQQMRFITLPLAWPVIGIA
                     LTFRLIGAFRSFDILYAVTKGGPGRATDTLIIQAYQESFSYFRFEYGLVVGIVMMLIT
                     IAMCAALLRRVTRKT"
     gene            complement(2405..3721)
                     /locus_tag="VEL68_13825"
     CDS             complement(2405..3721)
                     /locus_tag="VEL68_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ37093.1"
                     /translation="MKRLSSLFLLFFFGALILAPAQGQAQPADYKSKINWQAYKGQTI
                     NVLFSTHPWQETITPFIPEFEELTGIKVNITKIPHDEMIVKVPAGFSSGTFAFDAFMG
                     RYYDSPKFTVEKWTAPIKEFLEDPKYTDSSWYNFKDFFPAAQAITTYGRYQDRLPITA
                     EASITVYRKDIYGDLGLKPAATFDELLQTVKKISESKKAMGITVRGGPALWMPLYGII
                     RSYGGDWYTKDDVVKMNSPESIAAVKMLAELAKYAPPGIAGFGWDQINTAMLSGVAAT
                     FIDASVIYPRLMDPTKSTVVGKIACAPYPTGPAGRVSNAHFWSIAMSNLSTKKPAAWY
                     FLQWATSRPTQKKVAIKGILPPRAAIWTDPDFTKAYQPDFIDAMNVTMKTAVCLPSGT
                     KAGLSILGLLDVQTRKVQEVILGQKEAKLAMDELTADWNKIIAGTK"
     gene            complement(4028..>4122)
                     /locus_tag="VEL68_13830"
     CDS             complement(4028..>4122)
                     /locus_tag="VEL68_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HXZ37094.1"
                     /translation="SRTAGLLLIPYFLWVSFATGLNLSIWWLNR"
BASE COUNT          895 a         1125 c         1287 g          815 t
ORIGIN      
        1 gtaggttccc gtcaccagaa aaggcaggaa ttcctgatcc aaggcgagtt tccctttgtc
       61 gatccgcagc ctccgcagcc ccaggaccac ggtggcctcc tttttctctt tcacccggtt
      121 gcatccgcag accaaagggg gctggtccca aggaaggcct tttagaatgg gatgctggga
      181 gacaacctcg ggaaccagcc cctggcacca gttgagacgg tcatcctctt tgaggcattc
      241 gaccggtagg acgggggcga gggtggaagt atggtaattg ccgatcagcc cgtgaaagga
      301 ctcccatcct ccgatcatca gcaaagaggt cccctgttcc accttgaggg cgatcttatc
      361 cagggcgcct gctgggaagt ttccgctggg ataatcgctc aggatgatga gaccatagtc
      421 cttttggagc agcctttcgg gaaagtgcat ctccattttc acatagtcga agccgatccc
      481 cgccagggtc attaccccgc cgagatagca ggctccggtg gccagggtgg catcgccgca
      541 gtacaggatt tctttctttt ccgtcatgac tttccccttt ctcacttgac agctcccatg
      601 gtaagacccc gcaccagatt cttctggatg gccacgacaa agatgagcat gggaagcacg
      661 atcaggatcc cggccgcaga caacaacccc cattccacgg cgaggtaacc cacgaattcg
      721 ctgatgatga aaaccggggt ggtgcgcacc tgcaggctgg tgagggtgag ggccatcatg
      781 agttcgttcc acgagaagag aaaacagaag atggtcgtcg ccgccaggcc aggggccgtc
      841 gtggggaggg caactctgta gaaggcctgg atattggagc agccgtccag cctcgccgcc
      901 tcctccaggc tgtcggggat gtcctgaaag aacccccgca tcatccatac caccagccca
      961 atgttgatga aagtgtgagc caggatcaaa cccacgtggg tgtcgataag gccgaagaac
     1021 tgaaaagccc ggttcagggg gatgatgatg acgatgggag acaccatacg ggtgatgagc
     1081 aggaaaaacc cgaccccctc cgatccccgg taccgcaggc gcgccagggc gtacccgcag
     1141 ggagtgccca ggagaaggcc gagaatgcta gaccccgcgg ccatgatgaa gctgtcccag
     1201 atcgaagcca ggaatccggg cttgaccagg acctggcgaa agttctccag gacgggggtg
     1261 aagaagacct tcggcggtac ggcgatggtg tcgaaatagg atttgaaggc ctgggtcccg
     1321 atccagagga tcggcgaaag ggcgacggcg cagatgagga tcatgacggc gtgtttcagg
     1381 aattcgatga ggttttgcct ggtgcgcggg tccatgagtc cctcctaagt cttccgcgtg
     1441 acccgccgga gaagggcggc gcacatggcg atggtgatga gcatcatgac gatccccacc
     1501 acgagcccgt actcgaagcg gaagtaggag aagctctcct ggtacgcctg gatgataagg
     1561 gtgtccgttg ccctccccgg gcctcccttg gtcacggcgt agaggatgtc aaaggagcga
     1621 aaggcgccga tgagacgaaa ggtgagggcg atcccgatca ccggccaggc caggggtagg
     1681 gtgatgaacc gcatctgctg ccaggccgag gccccgtcga tccgggccgc ctcgaagggc
     1741 tcctggggca gcatctgcag gccggcgtaa atgacgaaaa gcgggaaggg ggtccactgc
     1801 cagctttcga tgatgatcac cgagatcatg gccgagttga tgcccgtgtg ccaaagcgag
     1861 gtgggcagcc ccatcgactg gaggaggtaa ttgatgatcc cgctcagggg attcatcatc
     1921 atctgccagg tcatgccgga aaccacggcg ctcatgccca gaggcaggat gaggatcgcc
     1981 cggtaaatcc acaccccctt ctcgatactg ttgaggagaa gggcgatccc cagcccgaag
     2041 cccacggtga agcctaccgt gcctaccgtg tagaccaccg tgttcttcat gcccagccag
     2101 aagagctggg aggtgaggat gtcccaatag ttcttcaggc ccacgtattg aaggttggaa
     2161 aggcctttgg aagtggagtg gaagctgagg tagattccgt aaagcagcgg atagacaaag
     2221 atggccacca ggaagaggag caccggcagg gtgaggatga gggccgcgcc ccgctccagg
     2281 tccattttca actttcccat ggattcccta tcctagacgc gaaaaaacat aaaccccttt
     2341 ggcccccggg cggtcgccaa agggggggaa ggcacgatca atcctgcggg ggcggtggcc
     2401 cgcgctactt ggttccggcg atgattttgt tccagtccgc ggtcagctca tccatggcca
     2461 gcttggcttc tttctgcccg agaatgacct cctggacttt gcgcgtctgg acgtccagca
     2521 gccccaggat ggagagtccg gctttcgtgc ccgagggcag gcacaccgcg gtcttcatgg
     2581 tcacgttcat ggcgtcgatg aagtcgggct ggtaggcttt ggtaaagtcg gggtcggtcc
     2641 agatcgccgc ccggggcggc agaatgcctt tgatggccac cttcttctgg gtcggccggg
     2701 aagtggccca ttgcaggaag taccacgccg ccggcttctt ggtggaaagg ttcgacatgg
     2761 cgatggacca aaagtgagca ttggacaccc ggccggccgg tcccgtggga tatggtgcgc
     2821 aggcgatctt tccaacgacc gtggacttgg tcgggtccat gagccgggga taaatgaccg
     2881 aggcatcgat gaaggtcgcg gccacgccgc tcagcatggc ggtgttaatc tggtcccatc
     2941 caaatccggc gattcccggg ggggcgtatt tggccagctc ggcaagcatc ttgaccgcag
     3001 cgatgctttc gggagagttc atctttacca catcgtcctt ggtataccag tccccgccgt
     3061 aggaccggat gataccgtac aggggcatcc acagggcggg accgccgcgc acggtgattc
     3121 ccatggcctt ctttgattcg ctgatcttct tcactgtctg gagcagctca tcaaaagttg
     3181 ccgcgggttt caggccgaga tccccgtaga tgtctttgcg gtagaccgtg atgctcgctt
     3241 ccgcggtgat cggtaaccgg tcctggtacc ggccataggt ggtgatggcc tgggccgcgg
     3301 ggaagaagtc cttgaaattg taccaggaag aatcagtgta cttggggtcc tccaggaact
     3361 ccttgatcgg ggcggtccac ttctccacgg tgaatttggg cgagtcgtag tatcggccca
     3421 tgaaggcgtc gaaggcaaag gttcccgagg agaacccggc cgggactttc acgatcattt
     3481 cgtcgtgggg gatcttggtt atgttcactt tgatccccgt cagctcctca aactcaggga
     3541 tgaagggggt aatcgtctcc tgccaggggt gggtggaaaa gagcacgttg atcgtctgcc
     3601 ccttgtaggc ctgccagttg atcttcgatt tgtaatccgc tggctgggct tgcccctgag
     3661 ccggagcaag aatcaatgcg ccaaagaaga agaggagaaa gagagaagag agtcgtttca
     3721 tcagctacct ccttgtttct cgcgggatgg gaacctacat aggagagatt gtatgaaccc
     3781 ggaaattata ctgtcaaggg gaaaaagctt cttcccctct ctgccaagag gctgtgtcgc
     3841 aaataagggg aagggcgtca ttccggcgaa agctcgtaga gtcgagtctc cgaccggaat
     3901 ccagtgttcg attttattaa atttatttga atcctggatg ccggcttccg ccggcatgac
     3961 gaattacgac acagtctcca gagggtgggg gtccttcatt ggaccgttcc ctctcgccag
     4021 agccaactta gcggttcaac caccagatgg aaagattgag gccggtagcg aaagagaccc
     4081 agagaaagta gggaatgagc aaaaggccgg cagtccggga ca
//
LOCUS       DASPRR010000370         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_235100, whole genome shotgun sequence.
ACCESSION   DASPRR010000370 DASPRR010000000
VERSION     DASPRR010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235100"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VEL68_13835"
     CDS             complement(<1..132)
                     /locus_tag="VEL68_13835"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HXZ37095.1"
                     /translation="MAGKIYHQLSCRDAGVDCDFLVRAETKEEVMRVAGDHACKSHEM
                     "
     gene            complement(246..1679)
                     /locus_tag="VEL68_13840"
     CDS             complement(246..1679)
                     /locus_tag="VEL68_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXZ37096.1"
                     /translation="MGKFEQYISYDAIGLAELVRKKEVKPIELVDAAIERIERLNPTL
                     NAVITPLFDQARKAAAGDLLQGPFTGVPYLLKDIGAMLGGVRMTMGTAFLQDFIPDHD
                     SELVIRLKRSGLIFVGKTSTPELGILPTTESRLLGRTRNPWNLDRTTGGSSGGSAAAV
                     AARIVPAAHANDGGGSIRIPASCCGVFGLKPTRARNPLGPDLGDMFGGLVAEHAVTRS
                     VRDSAAILDATSGPDIGDPYWAPPPARPFLQEVGANPGKLRIAFTASGVAGIDVHPDC
                     VEAVNDAAKHCADLGHKVEEASLPANRDLINQAFMVLWTAGCAWTIDGLAMARRKKPS
                     SGEFEPLTWALYEMGKRQTASSYLLSLTLLQRIARDLARFSLKYDVWITPTLAEPPVP
                     LGTFDSPAENPLHGYRRAEKFVPFTPICNVTGQPAMSVPLFWNREGLPVGTHFIGRFG
                     DEATLFRLAAQLECARPWAGRRPPVSV"
     gene            complement(1817..2923)
                     /locus_tag="VEL68_13845"
     CDS             complement(1817..2923)
                     /locus_tag="VEL68_13845"
                     /inference="COORDINATES: protein motif:HMM:NF022789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HXZ37097.1"
                     /translation="MLRLGPGGEDLLGKKNRPGCPLPGGFDRLPQDAAGGTGAWPPAR
                     PWHEIEGHPQVALFSIRPRSGLIDFERFTVFYTSGSAGGQIVHIAFAALPWTRPRRIG
                     AIVDPFFLKLLLAFGVGGLMVTLATVAAERLGSKMGGLIGGLPTMVATSLFFIGYAQT
                     PQTASEATSTIPLIMGFNGIFLVIYVILAKKGAWVGMSGALVLWFALAWAVLFLRIDS
                     FSISLLIFIFSLIGTYHLLENRFRLPSTGRLSVRYTPFQIISRALFSGLVVSAAVYLS
                     KVGGPIWGGVVAPFPTVYISTLIIMAKSKGAEFSRGIAKSLLVSGMINVVVYASAVRF
                     FYPLFNLALGTILSLAITAASAYGTYWFMKNKMR"
     gene            complement(3014..>3204)
                     /locus_tag="VEL68_13850"
     CDS             complement(3014..>3204)
                     /locus_tag="VEL68_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37098.1"
                     /translation="HRGDWRTFNFYYLAACLAANLGHMIRAYQADKSESILKSAADTA
                     HYAMMISDLCGSLTARKK"
BASE COUNT          712 a          924 c          925 g          643 t
ORIGIN      
        1 catttcgtga gatttgcagg catggtcgcc cgcgactctc atgacctctt ctttggtttc
       61 ggcacggacc aggaaatcgc aatccacgcc ggcatctcgg caactcagtt gatgataaat
      121 tttgcccgcc atggtgataa ccccctttcc ttatgggcat atatgtactc gttttattca
      181 gggctcggaa aaccctttac tttttggatg aggaaaattc ttttttgatt cccctcagat
      241 tcagcttaaa cggaaaccgg cggtcgccgc ccggcccagg gccgtgcgca ttcaagctgg
      301 gcggccagac ggaataaagt ggcctcgtcg ccgaacctcc cgatgaagtg ggtccccacc
      361 ggcagcccct cccggttcca gaagagggga acggacatgg ctggctggcc ggtgacgttg
      421 cagatgggag tgaaagggac aaacttctcc gcccgccggt aaccgtgcag gggattctcc
      481 gcgggagaat cgaaagttcc caggggtacc ggaggctcgg ccaaagtggg ggtgatccat
      541 acgtcatact ttagagagaa acgggcgagg tctcgggcga tcctttgcag cagggtgaga
      601 gaaagaagat aactggatgc ggtctgcctt ttccccattt catagagagc ccaggtgagc
      661 ggctcgaact cacctgaaga gggtttcttc cttctggcca tggccagccc atcgatggtc
      721 caggcgcacc cggccgtcca aaggaccatg aaggcctggt tgatcaaatc tctgttggcc
      781 ggcagggagg cttcctccac cttgtgtccc aggtctgcgc aatgcttagc cgcatcattt
      841 accgcctcta cgcaatcggg atggacgtcg atccctgcaa ctcctgaggc cgtaaaggcg
      901 atccggagct ttcctgggtt ggcccccact tcctggagga acgggcgagc tgggggagga
      961 gcccagtagg ggtcgccgat atccgggccg gaggttgcat ccaggattgc agcgctgtcc
     1021 cgcactgagc gggtcaccgc atgttccgcc accaaccctc cgaacatgtc tcccaaatcc
     1081 ggccccaagg gattccgcgc gcgggtcggc ttcagaccga agaccccgca gcaagaggcc
     1141 ggaattcgga tcgacccgcc gccgtcattg gcatgagcgg caggcacgat tcgggccgcc
     1201 acagccgctg ccgaccctcc gctagacccc cctgtagtcc gatccaggtt ccagggattg
     1261 cgggtgcggc ccagaaggcg ggattcggtg gtgggcagaa tacccaattc aggtgtgctg
     1321 gtcttcccga cgaaaatcaa gccggagcgc ttcaggcgaa tcaccagttc actgtcgtga
     1381 tcgggaatga aatcctggag gaaggctgtg cccatggtca tgcggacccc acccagcatg
     1441 gcgccgatat ccttcaggag gtaggggact ccggtgaaag gaccttgaag caggtcccct
     1501 gcggccgcct tccgggcctg atcgaagaga ggcgtgatca ccgcattgag ggttggattc
     1561 aaccgctcga ttcgttcgat ggcggcatct accaactcaa ttggtttcac ctcttttttc
     1621 cggaccagct cggccagccc tatggcatcg taagaaatgt attgctcgaa ctttcccaag
     1681 gccatccctc ctattcttga aaatttgatg ttcaggtcta tgaatcaaag gaccggcgga
     1741 ttattcgggc ggaaactccc gcattgttcc attattcaat tccgccttcc gcactgcgaa
     1801 ttccgcattt cagctgtcat ctcattttgt tcttcataaa ccaataagtc ccatacgcgc
     1861 ttgccgccgt tatcgccaag gacaggatgg tgccaagagc caggttgaac aaggggtaaa
     1921 aaaaacgaac cgcgctggcg taaacgacca cgttgatcat tccgctgacc agcagagact
     1981 tggcgatccc ccgggagaac tccgcaccct tggatttggc catgatgatc agggtcgata
     2041 tgtaaacggt cgggaatggg gcgacgacgc cgccccagat gggccctccc actttgctta
     2101 agtacacggc ggcggatacg accaatccgc tgaaaagggc ccgggagatg atctggaagg
     2161 gagtgtagcg gacgctcagt cttcccgtag aagggaggcg gaatcggttt tcgagaaggt
     2221 gataggttcc gatcagagaa aaaataaata tcagcagaga gattgagaaa ctatcgattc
     2281 tcaggaaaag aaccgcccac gccagggcaa accacaggac cagcgccccc gacatcccca
     2341 cccaggctcc cttcttagcc aggatcacat agatcaccag aaagattccg ttgaaaccca
     2401 tgataagagg aatcgtactg gttgcctcgg aggccgtttg gggggtctga gcatatccga
     2461 taaaaaagag agaagtcgcc accatggtcg gcagtccccc gattaagcca cccattttgc
     2521 tgcccagcct ttcagcggca acggtggcca gagtgaccat caggccgcct accccgaagg
     2581 ctaaaaggag tttcagaaaa aaggggtcca ctattgctcc tatccttctt ggccgggtcc
     2641 agggtaaagc tgcaaaggct atgtgtacaa tctgacctcc ggcagagccg gaggtgtaaa
     2701 aaacagtgaa ccgctcaaag tcgatgagac ccgaccgcgg gcggattgaa aagagggcca
     2761 cctgagggtg gccctcgatt tcgtgccagg gccgggcggg cggccaggcg cccgttccgc
     2821 ccgccgcatc ctgcggaagc cgatcgaagc cccccggaag agggcagccc ggccgattct
     2881 ttttgcctag aaggtcctcg cccccgggcc ccaaccgaag catcctggaa atgtcaatcc
     2941 ccctcaggcg gacccccggc atagccgggt aacagcaatt gggatttgct gccgcgtctg
     3001 gcgagattgg agtttatttt ttccgggctg tcaaactccc gcagaggtcg gagatcatca
     3061 tcgcgtaatg cgccgtatcg gctgcggatt taaggatcga ctcagattta tcggcctgat
     3121 aagcccggat catgtggccg aggttggccg ccagacaggc ggcaaggtag taaaagttaa
     3181 aggtcctcca gtccccccgg tggg
//
LOCUS       DASPRR010000371         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_402650, whole genome shotgun sequence.
ACCESSION   DASPRR010000371 DASPRR010000000
VERSION     DASPRR010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402650"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VEL68_13855"
     CDS             complement(<1..211)
                     /locus_tag="VEL68_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ37099.1"
                     /translation="MMKLKGKVALITGAARAIGRTHALRLAGLGADIVVNDINLESYK
                     EFEEKITAPTVVEELNALGVRSIGIE"
     gene            complement(406..1431)
                     /locus_tag="VEL68_13860"
     CDS             complement(406..1431)
                     /locus_tag="VEL68_13860"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ37100.1"
                     /translation="MKAVVKTQKGPGLEILEVKVPEVRDTDILIKVRAGSLCGSDVHI
                     YEWTAGYDWMPLPLTIGHEFSGEVVKVGSKAAAAVGDRVTVMPMMPCSNCPQCQVGKG
                     DSCTEKVILGLRADGVFAEYVRITAGATFFKLPENLSFEAAALCEPLCVALKAIDLSG
                     IKPGERAAVLGPGPIGLLTLELLNASGAGFIFVAGTKADCGRLEMARKLGADVTVEVD
                     QEDPVQKALTLTGTGFDFVFEASGNPRSVPQALNMIKPGGKVILIGIHSGPAQFNPTD
                     LVRGKKSIIGAYGYEPETWQRALTLLSSRRVKAEEIITHRVPLAEARRGFELAVKREA
                     AKVIFIP"
     gene            complement(1484..2188)
                     /locus_tag="VEL68_13865"
     CDS             complement(1484..2188)
                     /locus_tag="VEL68_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37101.1"
                     /translation="MLNVEKISAYYGDVQALRDVSLEVPERKIVALLGPNAAGKSTTL
                     RAISNVIRIRSGRISFHGERIDPLPLYRIVEMGIIHVPEGRGLFSTLTVRENLEMGAY
                     SARARGHLRESMGQVLDTFPVMKERQAQLAGSLSGGEQQMCSIARGLMARPRLLMIDE
                     MSLGLAPLIVQQMFEIVRRIAETGITVLLVEQQIQRALQTADAAYILESGATVLSGTT
                     ADLARHEHVKRVYLGR"
     gene            complement(2181..2906)
                     /locus_tag="VEL68_13870"
     CDS             complement(2181..2906)
                     /locus_tag="VEL68_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37102.1"
                     /translation="MSLLQINGLTKHFGGLVAVSDMSFEIEEGEIVGLIGPNGAGKTT
                     VFNLISGIFPPDRGRIFLGGQDITPLKLHVKSQLGIGRTFQIVRPFENMTVLENVMVP
                     ILARFQELGEAREAASTVLRQLGLSAQAFSEPQSLTYAQRKRLEVARALATKPKILLL
                     DEVLAGLNPVEVEEALPLVRHLRDSGVTIFIIEHVMAALMSVSDRVLVMDQGRLIASG
                     TPSEVTANPQVIKAYLGEEISRA"
     gene            complement(2906..>3423)
                     /locus_tag="VEL68_13875"
     CDS             complement(2906..>3423)
                     /locus_tag="VEL68_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37103.1"
                     /translation="LFLILAAASVYLARRIYHSALGHHLRAVAADEEAAQALGVNTSR
                     AQIIALLWSAGFTGMLGVFYAQFVYIIDPDFAFSSMLFSVQPALNGIIGGMGTIWGPV
                     LGSVLMTPLGEYLRSYLGHLQQGLSFFIYGMALIAVVMIMPGGIVSVLTPYFRRKVPA
                     SKDISERKAVS"
BASE COUNT          722 a         1035 c          978 g          688 t
ORIGIN      
        1 cttcaattcc gatggagcgt acgcccaggg cgttcagttc ctccactact gttggagcgg
       61 taatcttctc ctcgaattcc ttgtaggatt cgagattgat atcattgacc acgatatctg
      121 cccccaggcc agccagacgc agggcatgag tacggccaat cgctctggcc gccccggtaa
      181 tcaaggcgac ctttcccttt agtttcatca tcccctccaa actttccgat acgcgaaatt
      241 aggctagatc gatgccacct tcagagattc ttagcaggat gcgggaaaac tcgacttatc
      301 cgtcactccc gcgaacgcgg gagtccagaa ggcattgaaa aaactggatt cccgcttccg
      361 cgggaatgac actggaagac tgaaaaagcg tttttccgca ccgtgtcagg gtataaaaat
      421 aacttttgct gcttccctct tcacagcgag ttcaaaccct cttcgggctt cggccaacgg
      481 gacccgatgc gtgatgatct cctccgcctt gacccttcgg gaagaaagaa gggttaaggc
      541 cctctgccat gtctccggtt catacccgta ggccccgatg attgattttt tcccacgcac
      601 cagatcggtg ggattgaact gggccggacc ggaatgaatt ccgatgagaa tgactttccc
      661 tcctggcttg atcatattca gcgcctgggg aaccgaccgg ggattgcccg aggcctcgaa
      721 gacgaaatcg aatccagtcc cagtcaacgt cagggctttc tgaaccgggt cttcctggtc
      781 cacctcaacc gtgacgtcgg ccccaagctt ccgggccatc tcaagccttc cgcagtccgc
      841 cttcgttccg gccacgaaaa tgaaaccggc cccgctggca ttcaacagct caagcgtcag
      901 gaggccgatg gggcccggac caagaacggc agcccgctcc cccggtttga tcccggacag
      961 atcgatggcc tttagggcca cgcacaaagg ctcacacagg gcggcagctt caaagcttag
     1021 gttttcaggg agtttgaaga aggtggcccc ggccgtgatg cggacatatt ccgcaaacac
     1081 accatcagct ctcaggccca gaatcacctt ctccgtgcag gaatcgcctt ttccgacctg
     1141 gcactgggga cagttgctac agggcatcat tggcatcacg gtcacccggt cgcccaccgc
     1201 cgccgcggcc ttggacccca ctttgacgac ttctccggaa aactcgtggc cgatcgtgag
     1261 cggtaggggc atccagtcat atcctgcggt ccactcgtag atgtggacgt cactgccaca
     1321 aagactcccg gcgcgaactt tgattaaaat atccgtgtcc cggacttccg ggacctttac
     1381 ctcgagaatc tcgagtcccg gtcccttttg cgttttgacg actgctttca tttgtttcgt
     1441 ccctatggaa tcgattccgg agaggcggac cgaaacgttc tcgtcacctt ccgaggtaaa
     1501 cccgcttcac atgctcatgc cgggcgaggt cggcggtggt gcccgaaagg acggtggccc
     1561 cgctctccag gatgtaggcg gcatcggctg tctgcagggc gcgctggatc tgctgttcca
     1621 cgaggagaac ggtaatcccc gtctctgcta tccggcgaac gatctcgaac atctgctgga
     1681 cgatcagcgg agctaggccg agggacatct catcaatcat cagcagccta gggcgagcca
     1741 tgagtcctct ggcaatggag cacatctgct gttccccgcc cgacagggac ccggccaact
     1801 gtgcctgccg ctccttcatg accgggaacg tgtccagcac ctggcccatg ctctcccgca
     1861 ggtgcccgcg agcccgcgcg ctgtatgcgc ccatctccag gttttcccgc actgtgagag
     1921 tactgaagag ccctcgacct tcaggcacgt gaatgatacc catctccaca atccgataca
     1981 ggggcaaggg atcaatccgt tctccgtgaa agctgattcg gccagaccgg atccggatta
     2041 cgttgctgat cgcccggagg gtcgtcgatt ttccggctgc gttcggcccc aagagagcca
     2101 cgatcttcct ctccggcact tccagggaga catcccgtag agcctgtacg tctccgtaat
     2161 aagccgagat cttctccacg ttaagcacgg gaaatctcct ccccgaggta agccttgatg
     2221 acctgcggat ttgccgtaac ttcggaaggc gtgcccgaag cgatcaggcg gccttggtcc
     2281 atgaccagca cgcggtcgga aactgacatg agggccgcca taacgtgctc gatgatgaaa
     2341 atggtgaccc cgctgtcgcg gaggtggcgg acgaggggca aggcctcctc tacttccacc
     2401 gggttcaagc cggcgaggac ttcatccaac aggagaattt tgggtttggt ggccagggcc
     2461 cgggcgactt ccaggcgctt gcgttgggcg taggttaggc tttggggctc ggagaacgcc
     2521 tgggcagaga gacctagctg ccgcagtacg gttgaagccg cctcccgcgc ctcccccagc
     2581 tcttggaagc gtgctaggat gggtaccata acgttttcga gaacggtcat gttttcgaag
     2641 gggcggacaa tctgaaaggt tcggccgatc ccgagctggc tcttcacatg gagcttcaag
     2701 ggggtgatgt cctggccgcc gagaaagatg cggccccgat cgggagggaa gatgccggat
     2761 atgagattga agacggtggt cttgcccgcc ccgttggggc cgatcaatcc aacaatttca
     2821 ccttcctcga tttcaaagct catatcggaa acggctacga gaccgccgaa atgcttggtg
     2881 aggccgttga tctgcaggag ggacatcagg agaccgcctt tcgttcggaa atgtccttcg
     2941 aagcaggcac ctttcgccgg aaatacgggg taagaaccga aacgatcccg cccggcatga
     3001 tcatcaccac cgcgatcagg gccatgccgt agatgaaaaa gctgagcccc tgctggaggt
     3061 ggcccaggta ggaccgcagg tactcgccca agggggtcat gaggaccgat cccaggaccg
     3121 gcccccagat cgtgcccatg cccccgatga tgccgttcag tgcgggctgg acagagaaga
     3181 gcatggatga aaaggcgaaa tccgggtcga tgatgtacac aaactgcgcg tagaaaaccc
     3241 ccagcatgcc ggtgaacccc gcgctccaga gaagggctat aatctgggcc cgggaagtgt
     3301 tgacccccag cgcctgggca gcttcctcgt cggcggcaac cgcgcggagg tggtgcccca
     3361 aagccgaatg gtagatccgg cgagccaggt aaacgctggc tgcggccaga atcaggaaga
     3421 ggt
//
LOCUS       DASPRR010000372         7251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_570426, whole genome shotgun sequence.
ACCESSION   DASPRR010000372 DASPRR010000000
VERSION     DASPRR010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570426"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..1160)
                     /locus_tag="VEL68_13880"
     CDS             complement(456..1160)
                     /locus_tag="VEL68_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37104.1"
                     /translation="MLEVRNLHVHYGSIRALKGISLEVKKGEMVALIGANGAGKTTLL
                     NTLSGFNRPKQGEILFRGREIRGLAPNEIVAQGMVQVPEGRSILTQMTVLENLEMGAY
                     IRKDSQEVRRDLDKTLERFPRLQERRAQLGGTLSGGEQQMLAIGRALMGRPQILLLDE
                     PSMGLSPVLVREVFRIIREIKAAGTTLLLVEQNARMALREADRGYVLENGSIVFQESA
                     GELLKSRLVQEAYLGA"
     gene            complement(1161..1928)
                     /locus_tag="VEL68_13885"
     CDS             complement(1161..1928)
                     /locus_tag="VEL68_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019098888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37105.1"
                     /translation="MVSPLLEVYQASRVFGGLRALDGVSLEVREGEVFGLIGPNGAGK
                     TTLFNLVSGALPVSAGRIFFRQNEISLLPDYRICQLGIARTYQNIRLFGKMTVLENVM
                     VGRHSRTRGGLWSAALRLPGDRAENARSRKIAEEWLGFFHLLAKKEEKASNLAYGEER
                     RLEMARALATEPSLLLLDEPSAGMNPQEVRDLNDLILQIRRQGKTIFIIEHNMRVIMD
                     ICDRIAVLDFGQKIAEGSPAEVRANPRVIEAYLGREA"
     gene            complement(1909..2775)
                     /locus_tag="VEL68_13890"
     CDS             complement(1909..2775)
                     /locus_tag="VEL68_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37106.1"
                     /translation="MFDAYTSQVLTFIGINSILALSLYITALAGQLSVGHAAFMGIGA
                     YTSTLLMTKLGWPFFLATASGALVGGFFGFCIGLPTLRLRDLYLAIATLAFNIIWVVV
                     VMNIEAFGGALGIFNVPKATNNTIVYLSLALLLLFFYRLKDSRLGRAFRAIEENEEAA
                     RAMGVNAVYYKVYAFTLGALLAAYAGALYAHFVTFISPHDFEVDRSIEILLFVVLGGA
                     QTFIGPVIGAVLLTLAPEFLRFLAEYRLLVYGLMFVGIMLVRHEGLIEENTFSWKRWS
                     KIFRRENRVPAP"
     gene            complement(2848..3711)
                     /locus_tag="VEL68_13895"
     CDS             complement(2848..3711)
                     /locus_tag="VEL68_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37107.1"
                     /translation="MLAQQLVNGLTLGSTYALVALGYTLVFGILGMINFAHGEVFMLG
                     AYIALLLVTIGHLDIFWSLIGAMAGAAVLGMIIERVSFRPLRKVSILAPLISTIGLSI
                     FLRSLAQVIFGAESTRFPVSIKTEFYQLGPFQISLLQIVILGISFGIMAVFQVFIMKT
                     KIGKAMRATSESLYTAGLLGIPVDRIIFLSFAVASALGGVAGVLLGLNFNAVSPLMGK
                     MVGLKGLTIIILGGMGNITGAMVGGLFLGFVEVLSVGYLTSSYRDAVAFALMFVVLLI
                     RPSGLFGRVVK"
     gene            complement(3739..4884)
                     /locus_tag="VEL68_13900"
     CDS             complement(3739..4884)
                     /locus_tag="VEL68_13900"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37108.1"
                     /translation="MKTGIIIGFCCLLAVCALGGEFSDASAAGEILIGNVSCLTGTAA
                     QYGISTYEGLEVAMDEINAAGGVLGKKISILKGDDSADPRQAVNVVQKFITKDKVLVI
                     IGAELTGTTRVTAPVSNQAQMVQFTASATGAGLTDNLPYVFRNALPEAYAIPFVVEAA
                     KKKFKTSSVVIITSYNLDYSVDLTKIYEKALAAEGIKVQDKISYAEGDIDFSAQVSKL
                     KALKPETVVMTGYYQESAQLMIEMRKQGINCPILGSNGFNDPKILDLAGKAMEGAIWA
                     SGFFPDNPDPKVKKFVARVQEKYKKKPNQFHAQAYDALYIIAEAIKKAGVTESTPENR
                     TKIRDALASITNFPGISGVHTFKPGKGDAEKSVFILTLRDGKYALLD"
     gene            5261..6526
                     /locus_tag="VEL68_13905"
     CDS             5261..6526
                     /locus_tag="VEL68_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37109.1"
                     /translation="MNQRRNFQAWVFFALFLGIAAVLIPGKGMAADSIPIGILLPYTG
                     PLGWVAACQTGADIARDEINAAGGVLGRQVQFFVADDEGKSDSAIAGEEKLININKAP
                     AIIGPTSVTFRSVMPIAIKNKVVQISPTAGTTALDFVKEKYVFRTVSSDVVMGTGMVF
                     EAAKRKYQKAALFFGDDESAQSIRGVLVPACKAAGIEIVGDVTFTQRQTSYRSELLSA
                     LKNKPQVIFFEADPQTSAVIFKQVTELKMAGDWIGTDFVSDQFLQATWPYSKGVGAVQ
                     PAAKTSPRLDAFAKEVEKRRGKPGIPVFTVNAYDAMIIMALAMEFAKKTDGESLAENI
                     RKVANPPGTKVYSFAEGVKLLRQGQKIDYEGLAGPQNFDEYGNVVTSLGVFKIEDNQR
                     KAIGFLDENDFGELLGKVRQLYATAQQKK"
     gene            6580..>7251
                     /locus_tag="VEL68_13910"
     CDS             6580..>7251
                     /locus_tag="VEL68_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37110.1"
                     /translation="MLALSIGFGIITGGVLALAALGLSLLFRTVKFINFAYGDVLALG
                     AYLTFFFNFTLGLPLVLSALLGMVSTGIVGVIIHKAVVKPLREGVKAGPLTLLIATMG
                     TAFIIRNAILIIWGPEPKMYNVATQEAMNLGPFLITPLQLMAIGISVLIMVLIYLLLQ
                     HTKLGKMIRATSDNIDLARIRGIRTEKVVVWTWLLSCGLAGLAGVMLGLMGAVDNHMG
                     IEMLLV"
BASE COUNT         1564 a         2124 c         2077 g         1486 t
ORIGIN      
        1 ctcagcatgg caaaatcctt tccggccctg ccccgcccga gtctggacgg gatgtctcgt
       61 gatttcttgg aaagcctttt tgttggagag gatcttcgga gagaaggatt ccgggagaaa
      121 aaggtagagg agaaagaacg atgctgattc cgcagaagaa tgacgtccgc gacatcttcc
      181 ctcctccccg ccggtgagca aaaagagaaa ttacggccgg cctttttaaa gtcaccttac
      241 catattacat cccaccttcc ctgtcaagaa aattttagcc taatgaaaat tcatttacta
      301 ttaccaaata ttaactcccc cgcctcgttg cgggttgcca gttcctggtt actggttgct
      361 ggttacccgt taaaggaaat aaatccttct ggaattggcc cgacccagcc catcctggga
      421 aacaagaaac aagcaaccaa gaaccagcaa cgatttcaag cccccaggta ggcctcctgg
      481 accagacggc tcttcaagag ctccccggcc gattcctgga aaacgatcga tccattttcc
      541 agcacgtacc cccggtcggc ctcccgcagg gccatccggg cgttttgctc cacgaggagg
      601 agggtggtcc cggcggcctt gatctcccgg atgatccgaa aaacctctcg gaccagaacc
      661 gggctgaggc ccatggaggg ttcgtccagg aggagaatct gcggcctgcc catgagggcc
      721 cgcccgatgg ccagcatctg ctgttcgccc ccgctgaggg tcccgccgag ctgggctctt
      781 ctctcctgga gtcgggggaa tctttccagg gttttatcca ggtctctccg aacctcctgg
      841 ctgtccttgc ggatgtaggc tcccatttcc aggttctcca gcaccgtcat ctgggttagg
      901 atggacctgc cttccggcac ctggaccatg ccctgggcga caatttcatt gggggcaagc
      961 ccgcggatct ccctgccgcg gaaaagaatc tccccctgct ttgggcggtt aaaaccggaa
     1021 agggtgttca acagcgtggt tttccccgcc ccgttggccc cgatcaaagc gaccatctcc
     1081 cctttcttga cttccaggga aatccccttg agggctcgga tcgaaccata gtggacatgg
     1141 aggttacgaa cttcgagcat tcatgcctcc cgcccaaggt acgcttcgat gacccgtgga
     1201 ttggcccgga cctccgccgg agacccttcg gcgatcttct gcccgaaatc aaggaccgcg
     1261 atgcggtcgc agatatccat gatcacccgc atgttgtgtt cgataataaa gatcgtcttc
     1321 ccctgtctcc ggatttgaag gatcagatcg ttgaggtcac gcacttcctg cgggttcatg
     1381 ccggcggagg gttcgtccag caagaggaga gaaggctcgg tcgcgagggc ccgggccatc
     1441 tccagccttc gctcttcccc gtaagccaga ttggaggcct tctcctcctt tttcgccagg
     1501 aggtggaaaa accccagcca ttcctccgcg atcttcctgg atcgtgcatt ctccgcccga
     1561 tccccgggaa ggcggagggc ggccgaccac agcccgcccc gcgtccggct gtgccggccc
     1621 accatgacgt tctccaggac cgtcattttt ccgaagaggc ggatattctg gtacgtacgg
     1681 gcgatcccga gctgacagat gcggtagtcg gggaggaggg aaatttcgtt ctgcctgaag
     1741 aatatgcgcc ccgcagaaac aggaagcgcc ccggaaacaa ggttgaagag ggtcgtcttt
     1801 cccgccccgt tcgggccgat taggccaaag acctcccctt cacgcacctc caggctgacc
     1861 ccgtccagcg ctcttagccc gccgaagacc ctgctggcct ggtacacctc aaggagcggg
     1921 gacacgattt tccctccgga agattttgga ccatcgcttc cacgaaaagg tattttcctc
     1981 aatcagccct tcgtggcgga ccaacatgat ccccacaaac atgagcccgt agaccaaaag
     2041 gcggtattca gccagaaaac gcagaaactc gggggccagg gtgagcagca ccgcgccgat
     2101 gaccggcccg atgaaagtct gggcgccccc cagaaccacg aagaggagga tttcgatgga
     2161 acggtccacc tcgaaatcat ggggggagat gaaggtgacg aaatgagcgt acagcgcccc
     2221 ggcataggcg gccagaagag ctcccaaggt aaaggcatac accttgtaat agacggcatt
     2281 tacccccatg gctctcgctg cctcttcatt ctcttcgatc gcccggaatg cccttcccag
     2341 cctggagtcc ttcaggcggt agaagaagag gagcaaaagg gccagggaaa gatacacgat
     2401 ggtgttgttg gtggccttgg gaacgttgaa aattcccagc gctccgccga aggcctcgat
     2461 gttcatcacc accacaaccc agatgatgtt gaaggccagg gtggcgatgg ccaggtagag
     2521 gtcccgaagg cggagcgttg gcagaccgat acagaaaccg aaaaagcctc ccaccagggc
     2581 tcccgaggcc gtggcaagaa agaaaggcca tcccagcttg gtcatgagca gggtggaagt
     2641 gtaggccccg atgcccatga aggctgcgtg ccccacggag agctgcccgg ccagggcggt
     2701 aatatagagg ctcagcgcca ggatgctgtt gatcccgatg aaggtcagta cttggctggt
     2761 gtaagcgtca aacatgaaaa cacagtgtca ttgtgagtgt gagtgtgatt ctcggcactt
     2821 ttccattccg cattccgtat ttcaaattta cttgacgact ctcccgaaca ggccgctcgg
     2881 ccggatcagg aggaccacga acatgagggc gaaggccacg gcgtcccggt aggaagaagt
     2941 taagtaaccc acactcaaaa cctccacgaa ccccagaaac aggccgccca ccatggcccc
     3001 ggtgatattt cccattcccc ccaggatgat gatggtcaaa cccttcagtc cgaccatttt
     3061 ccccatgagc ggagagacgg cgttgaaatt cagccccaga agaaccccgg ccacccctcc
     3121 cagggccgat gccacggcga aggaaagaaa gatgatccgg tccaccggga ttcccaggag
     3181 tcccgcggtg tacaggcttt ccgaggtggc ccgcatggcc tttccgatct tggtcttcat
     3241 gataaagacc tggaagacgg ccatgatccc gaagctgatg cccagaatca cgatctggag
     3301 aagggaaatc tggaaggggc ccaactggta gaattcggtt ttaatgctca ccggaaagcg
     3361 cgtggattcg gccccgaaga taacctgggc caggcttcgc aggaagatgg agagcccgat
     3421 ggtgctgatc aggggagcga ggatggacac cttgcgcagg ggccggaagg agacccgctc
     3481 gatgatcatg ccgagaacgg ccgccccagc catcgctccg atgagcgacc agaagatgtc
     3541 caggtgcccg atggtcacga ggaggagggc gatgtaggcc cccagcatga agacctcccc
     3601 gtgggcgaag ttgatcatcc ccaggatacc gaacaccagg gtatacccca aagccacgag
     3661 ggcataggta gaccccagag tcagcccgtt tacgagttgc tgggcaagca tgtcgggtgg
     3721 aatttcatcc tggcgaattt aatccaggag tgcgtatttt ccatcccgga gggtcaggat
     3781 gaagaccgat ttttccgcat ccccctttcc cggcttaaat gtgtgcacac ccgaaatccc
     3841 ggggaaattg gtgatgctcg ccaaggcgtc tcgaatcttg gtgcggtttt ccggcgtgct
     3901 ctccgtcacc cctgcctttt tgatcgcctc ggcgatgatg tacagggcat cgtaagcctg
     3961 ggcgtgaaac tggttgggtt tctttttgta cttttcctgg acccgggcca cgaatttctt
     4021 gaccttgggg tccgggttgt ccgggaagaa gcccgaggcc cagatcgccc cttccatggc
     4081 ctttcccgcc aaatcaagga tcttcggatc attgaacccg ttggagccca ggatgggaca
     4141 gttgatcccc tgcttgcgca tctcgatcat cagctgggcg ctctcctggt agtacccggt
     4201 cattaccacc gtctcgggct tgagggcttt cagcttgctc acctgggcgg agaagtcgat
     4261 gtccccttcg gcgtagctga tcttgtcctg gaccttgatc ccctccgcgg ccagcgcctt
     4321 ctcgtaaatc ttggtcaaat ccactgagta gtccaggttg taggaagtga tgatcaccac
     4381 gctgctggtc ttgaacttct tcttggccgc ctccaccaca aaggggatgg cgtaggcttc
     4441 gggcagggca ttgcggaaaa catagggtag gttgtccgtc aacccggcgc cggtggccga
     4501 agccgtgaat tggaccatct gggcctggtt ggagacggga gcggtcaccc gggtggtccc
     4561 ggtcaactcg gcgccgatga tgactaaaac cttatctttg gtaatgaact tctggaccac
     4621 gttgaccgcc tgccgggggt ctgccgagtc gtcccccttt aagatagaga tctttttgcc
     4681 caggaccccg ccggccgcat tgatctcatc catggccact tcgaggccct cataggtgga
     4741 gatcccgtat tgggcggccg tgccggtcaa acaagagacg ttgccgatga gaatctctcc
     4801 cgccgccgaa gcgtccgaga attcaccccc tagggcacaa acagccagga gacagcagaa
     4861 tccgattatg attcctgttt tcatcttctt cctccttccc cttagaattg cagccaaagt
     4921 attatactgt aggggctctg tcaagaaatt tttggccgca actccgttta ggccgagacg
     4981 aaatttgaat tgacaagccc cgtcgatttt tggtaaagag gggtaaattc ttttacgttt
     5041 tcgatcatgg cagcctattc cgtcgcgggt tcagaagaag gggtgaacaa ttgaagagga
     5101 ccggcttcca ggtcgatcta cgcattgctg gcaaacaaac aagcaaacgg gagtgtccgt
     5161 cggatcaatc ccttcgcagg aaaagggagg gagttctttc ccgggatttt ttggggggca
     5221 ggagagtttc tgcccccctg aaaccgaaag gagaataacc atgaaccaga gaaggaattt
     5281 tcaggcttgg gtctttttcg ctcttttcct gggcatcgcg gcggttctga ttcccggaaa
     5341 gggaatggct gccgactcca ttcccatcgg gatcctgctc ccatacaccg gccccctggg
     5401 atgggtggct gcctgccaga ccggggccga catcgcccgg gatgagatca acgccgcagg
     5461 aggggtcctg gggcggcagg tccagttttt cgtcgccgac gacgaaggca agagcgacag
     5521 cgccattgcc ggagaagaaa agctcatcaa catcaacaaa gctccggcca tcatcggtcc
     5581 tacttcggtc actttccgtt cggtcatgcc catcgccata aaaaacaagg tggtgcagat
     5641 ttcccccacc gccgggacga cagccctgga ttttgtgaag gaaaaatacg tttttcgcac
     5701 ggtatcctcg gatgtggtca tgggaaccgg aatggttttt gaggcagcca aacggaagta
     5761 ccaaaaggcg gccctcttct tcggggatga tgagagcgcc cagtccatcc gcggggttct
     5821 ggtgccggcc tgcaaagccg ccggaatcga gatcgtgggc gatgtgactt tcacccagcg
     5881 ccagacctcc taccgttccg agctcctctc cgccctgaag aacaagcccc aggtcatctt
     5941 cttcgaggcc gacccgcaga ccagcgcggt gatcttcaag caggtgaccg agttgaagat
     6001 ggcgggggac tggatcggga ccgacttcgt gagcgatcag ttcctgcagg ccacctggcc
     6061 ctacagcaag ggggtggggg cggtgcagcc ggcggccaaa acgagccctc gcctggatgc
     6121 cttcgccaag gaagtggaga agcgcagggg caagccggga atcccggtct tcaccgtgaa
     6181 tgcttacgat gccatgatca ttatggctct ggccatggag ttcgccaaaa aaacggacgg
     6241 ggagtccctt gcggagaata tccgcaaggt ggccaacccc ccggggacca aggtctacag
     6301 ttttgccgag ggagtcaaac tcctccgcca gggccagaag atcgactatg agggtctggc
     6361 cggcccgcag aacttcgacg aatacggaaa cgtggtgacc tcgctgggag tcttcaagat
     6421 cgaggacaac cagcgcaaag ccatcgggtt cctggatgag aacgacttcg gagaactgct
     6481 gggcaaagtc aggcagcttt acgccaccgc ccagcagaag aaataggaag ggatggcggg
     6541 tgagggcttc gggcaagccc tcacccctca aaatagcgaa tgctggctct atccatcggc
     6601 ttcggaatca tcaccggagg ggttctggct ctggccgccc tgggtttgtc cctcctcttc
     6661 cggacggtga agttcatcaa cttcgcctat ggtgatgttt tggccctggg agcctatctg
     6721 acctttttct tcaatttcac cctggggctg cccctggtgc tttccgccct cctggggatg
     6781 gtctccaccg gaatcgtggg ggtgatcatc cacaaggcgg tggtcaagcc gctccgcgaa
     6841 ggggtcaaag ccgggccctt gaccctgctc atcgccacaa tgggcacggc cttcatcatc
     6901 cgcaacgcca tcttgatcat ctggggcccg gaacccaaga tgtacaacgt ggcaacccag
     6961 gaggccatga acctaggccc cttcctgatc acccccctcc agctcatggc catcggaatc
     7021 tctgtgctga tcatggtcct gatctatctc ctcctccagc acaccaagct gggaaagatg
     7081 atccgcgcca cttccgacaa tatagacctg gcgcggattc gggggatccg cacggaaaag
     7141 gtggtcgttt ggacctggct tcttagctgc ggcttggcgg gcctggccgg ggtgatgcta
     7201 ggcctcatgg gcgcggtgga caaccatatg gggatcgaga tgctcctggt g
//
LOCUS       DASPRR010000373         5873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_235317, whole genome shotgun sequence.
ACCESSION   DASPRR010000373 DASPRR010000000
VERSION     DASPRR010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235317"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VEL68_13915"
     CDS             complement(<1..1356)
                     /locus_tag="VEL68_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXZ37111.1"
                     /translation="MASFETLFSPFTIGNLRLKNRILMAAMGNNFSHPDGTVSDRAIA
                     YYAARALGGASLIITETSPVSLPGRHRTRGICAYDDSFLPGLQRLTDVVHGQGGAMAL
                     QLHHAGRLADPGITGVPAQAPSPIARTAGGPVPKEMSPDEIEATIADFAAAALRAKRA
                     GFDAVEVHGAHGYLIHQFLSPRLNHRKDSYGGSAENRGRLALEVLRRVRKEVGKAFPV
                     IFRLSVQEYVEGGYPLEEAVDWAIEAERAGASALHISGGTTESLPGAARVIPPMAFPE
                     GFHLPLAAAIKPHVRTPVIAVGRLGNPEVAERVLREGPADLIAAGRAFLCDPHWPRKA
                     LQGDADRIRPCVACNYCIWRLFQQEDLTCFQNALLGNEYQYRMEPAEKAKKVLVVGGG
                     PGGLEAARVARERGHRVTLWEKSPSLGGQMRLASIPPHKQTFRRALDWLTREAERGGI
                     EI"
     gene            complement(1590..2294)
                     /locus_tag="VEL68_13920"
     CDS             complement(1590..2294)
                     /locus_tag="VEL68_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37112.1"
                     /translation="MKGGKGEPKGVLAIWNDVDPKIETDYHEWYWQEHLLERLAIPGF
                     LTARRYEAAGSGPKYFSCYLTTSVEVLRSPAYLQRIHNPTEWTRRCMASFRNMNRTAC
                     RQTIDLGRGIGSAAVTMEIEAAAGQEDGLRRKISDSLFPDLLKSPGATGFLRCQLWEG
                     DPGITDQKTAEQALRGGTDKMVNWVVVIETSLLEQAEVAGQRLSAYPFTQGGANKIGP
                     PHVYRLLHYLQGPDGR"
     gene            complement(2314..3957)
                     /locus_tag="VEL68_13925"
     CDS             complement(2314..3957)
                     /locus_tag="VEL68_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXZ37113.1"
                     /translation="MEKRRLTQSYCQVLGINWTKGKTLGQMLKETAARHPDRPAILFQ
                     DQSYTYREFQGAVDSFSQALIDLGLRREDKLGLLLPDWPEYSIALYACAQLGVTVSPM
                     NPIYRKMEVLTVLNHLEAKALVIPEEWRDFRFVDLLEEVRTQIPSLQHIIVQGKPAKG
                     MHSFQALLSQDWGKKFGAGFLEKYLQDHPVEADDFLEIAYTSGTTGRPKGVVETHNTR
                     MLHSVGIAERMKATEKDVWLNMTPLFHTTGNCVVQHTVFLTGGTLILLGRYSTEISLR
                     EIDRCKATIAVGVPTMFIDLMNHPRFKKTGISSLRFALFTGAPMPPEVALQIVQKFKC
                     GILQGDGTTECGSNVLSLPDSPIELIAKSPGPPLAVGNEVKIVDPNNRIVPVGVPGEI
                     CHRGPTNFLGYYKEPELTAAVVDRAGWFHSGDLGTMDEEGNIRLIGRIKDMIIRGGEN
                     LYPTEIEGILYTYPQVKECQVVGYPDERLGEKTVACIIPKNAGEVVTREEIYEFMKDK
                     TARFKIPDMVITMEDYPRTASGKVQKFKLREIALEKIKK"
     gene            complement(3967..5619)
                     /locus_tag="VEL68_13930"
     CDS             complement(3967..5619)
                     /locus_tag="VEL68_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ37114.1"
                     /translation="MEKPVMSYYYSPGEIPPGVPLGEYFEQTAARHSGRVALIFRDRR
                     LTWGELHLLVERLTLAFIHLGVRRGDMIAILFPNRPEFIISLLATARLGAIAVPISER
                     LRKKEIEYILRQTEAKAAVSVSEFWGFSFSDLFQELREAIPTLKHVIVSGQKRHPEEI
                     PLEDLLNQEWEKRYPPHYYREVYQKDFPVEPDDLLEIIFTSGTTGNPKGVMHTHNTRC
                     RSALGTATSIQLHPDDVWLIMVPLSHTTALVNAFYASVIKGSCQVLLETWNVEEAMKE
                     ITRNRVTLPVGVPTMPIMMLQRPDFDRYDFSSVRAMVLAGAPLPIEVARQIIQKMGCY
                     VTSAYGMTETAMSNITRLDDPVEVVCETVGRPQPGMEHKVVDEKRRIVPLGVKGEACA
                     RGQNVCIGYFKDPERTAQTIDDRGWIHSGDLATMDENGNLRIVGRIKDMIVRGGENIS
                     PTEIEDILYTYPKVAQVSVVAIPDERLGEKTCACIIPKPGGPITQEEVKAFFKDKVAH
                     FKIPDRVELMSEFPMTPSGKIKKNVLREIMAQKVQEEKRKKG"
     gene            complement(5713..>5873)
                     /locus_tag="VEL68_13935"
     CDS             complement(5713..>5873)
                     /locus_tag="VEL68_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018180795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ37115.1"
                     /translation="GAKIVEAMKNAVPFRRLAKPEDISGAVAFLASDEAGFITGQTLS
                     VSGGLTMS"
BASE COUNT         1134 a         1724 c         1702 g         1313 t
ORIGIN      
        1 aatttctatc cctccgcgct cggcttcgcg ggtgagccag tccagagccc gacggaaggt
       61 ctgcttgtgg ggggggatcg atgccaggcg catctgcccg cccaaagatg ggcttttttc
      121 ccagagggtc acccggtgcc cccgctccct agccacccgg gcggcttcca gtccgccggg
      181 ccccccgccg acgaccagga ctttcttcgc cttttccgcc ggttccatcc ggtattggta
      241 ttcgtttccc agaagagcat tctggaaaca ggtcaggtct tcctgctgaa agagtctcca
      301 gatacagtag ttacaggcca cgcaagggcg gattcgatcg gcatccccct ggagtgcctt
      361 gcggggccag tgggggtcgc agaggaaagc tctgccggcc gcgatcagat cggccgggcc
      421 ttccctcaaa actcgttctg ccacctccgg gtttcccaga cggccgaccg cgattaccgg
      481 ggtccgcaca tgtggcttga tggctgccgc cagggggagg tgaaagccct ccggaaaagc
      541 catgggggga atcacccggg ctgcgccggg aagggattcc gtcgtccctc ccgagatgtg
      601 aagagccgag gctcccgctc gctccgcctc aatggcccag tccacagcct cctccagagg
      661 ataaccaccc tcgacgtatt cctggacgct caggcgaaaa atgaccggga acgcctttcc
      721 cacttctttc ctcaccctcc gcaggacctc gagagccaaa cggccccggt tctctgcgct
      781 tcctccgtag gagtctttcc tgtggttcag ccggggggaa agaaattgat ggatcaggta
      841 accgtgagcc ccatggacct ccacggcgtc aaacccggct cttttggccc ggagcgcagc
      901 ggccgcgaaa tcggcgatgg tcgcctcgat ttcatccggg ctcatctctt tgggaacagg
      961 acctcccgcc gttcgggcga tgggcgaagg ggcctgggca ggaacaccgg taatcccggg
     1021 gtcggccagc cttccggcgt gatgcagttg cagcgccatg gccccacctt ggccgtggac
     1081 cacgtcggtc aggcgctgga ggccgggcag aaaggaatcg tcgtaggcac agatcccacg
     1141 ggtgcgatga cgtccgggga ggctcacggg gctggtttcg gtaatgatca aactggctcc
     1201 ccccagagcc ctggcggcat agtaggcgat ggcccgatcg ctgaccgtcc catccggatg
     1261 ggaaaaatta tttcccatgg ccgccatgag gatacggttt ttcagccgaa gattcccgat
     1321 cgtaaaagga gagaagagcg tttcaaaact ggccatggat gctttaaggc gattctctgg
     1381 gtatgaatta tgggtctttg aatacattcg tctccgtaac cattttcctg tccgctaaaa
     1441 gcatggttgc ggagaactat aaaacgctga aagttggaag accccaaccc tatcaagccg
     1501 gaaccaaaca gaatatggta ggggccgccc cctgtggccg cccgcaaaat gggcaaccac
     1561 ggggggttgc ccctacagtt tgctaccatt caacgcccat ccggaccctg caagtagtgc
     1621 aggaggcgat agacatgggg tggaccgatc ttattggcac ccccttgggt aaaaggataa
     1681 gctgagagcc tttgccctgc cacctcagcc tgttcgagca aagaggtttc gatgaccacc
     1741 acccagttaa ccatcttgtc cgtccctccc cgtagggctt gctcggccgt tttctgatca
     1801 gttatccccg ggtctccctc ccagagctga cagcggagga acccggtggc accaggggac
     1861 tttagtaaat cggggaatag tgaatcagat atcttccgcc gcaggccatc ttcttgccca
     1921 gccgcggcct cgatttccat ggtcaccgcc gcactgccga tccctctccc caggtcgatg
     1981 gtctggcggc aggcggtccg gttcatgttc ctgaaagaag ccatgcatcg acgggtccat
     2041 tccgtcggat tatggattcg ctggaggtat gcggggctcc tcaaaacttc tacggaggtt
     2101 gtcagataac aggaaaaata tttcggcccg ctccccgccg cctcgtaccg tcgggcggtg
     2161 agaaagcctg gaatcgccag ccgctccaga aggtgttctt gccaatacca ttcgtgataa
     2221 tcggtttcaa tctttgggtc cacatcattc cagatggcca gcactccctt tggttcaccc
     2281 ttcccgccct tcattttctg ctccctcccc acctcatttt ttgatttttt caagagcgat
     2341 ctccctgagc ttgaatttct gcaccttccc gctggcggtc cgcgggtagt cttccatggt
     2401 gatgaccatg tcggggatct taaaccgggc ggttttgtcc ttcatgaact cgtagatttc
     2461 ttcccgggtc accacctccc cggcattttt cggtatgata caggccaccg tcttttctcc
     2521 cagccgctca tctgggtatc ctactacctg gcattctttc acctggggat aggtgtaaag
     2581 gattccttcg atttcggtgg ggtagaggtt ctctcctccc cggatgatca tgtctttgat
     2641 ccgtccgatc aggcggatgt tcccttcctc atccatggtc cccaggtctc cgctgtgaaa
     2701 ccatccagcc cggtccacaa cggcagcagt gagttcgggt tctttgtaat agccaagaaa
     2761 attggtcggg cctcggtggc atatctcccc cggaaccccg acgggaacga tgcggttgtt
     2821 ggggtctacg atcttgactt cgtttcccac cgccaggggc ggtcctgggc ttttggcgat
     2881 gagttcgatg ggcgaatcgg ggaggctgag aacgttactt ccacattcag tggttccgtc
     2941 cccctggagg atgccgcatt tgaatttctg gacaatctgc aatgccacct ccggcggcat
     3001 gggtgccccg gtgaaaaggg caaagcgcag ggaggagata ccggtcttct tgaatcgggg
     3061 atggttcatg aggtcgatga acatggtggg aaccccgacg gcgatggtgg ctttacagcg
     3121 gtcgatttcc cgcagggaga tctccgtgct gtaccgtccc aggaggatca aggtcccccc
     3181 ggtgaggaag acggtatgct gaacgacgca gttccccgtg gtatggaaga ggggggtcat
     3241 gttcaaccac acatcctttt ccgtggcctt catccgctcg gcgattccca cggagtggag
     3301 catccgggtg ttgtgcgtct ccaccacgcc cttgggccgg ccggtcgttc ccgaggtata
     3361 ggcgatctcc agaaaatcgt ctgcttccac ggggtggtcc tggagatact tttccaaaaa
     3421 accggcgccg aatttctttc cccagtcctg ggaaagcaaa gcctggaagc tgtgcatccc
     3481 tttcgcgggt tttccttgga cgatgatgtg ctggagagag ggaatctggg tgcggacctc
     3541 ttccagaagg tcgacaaagc gaaaatcccg ccattcttcg gggatgacca gggctttggc
     3601 ctccagatgg ttcagtacgg tgagcacctc catcttccgg tagatggggt tcatggggga
     3661 aaccgtgacc cccagctggg cgcaggcgta aagggcgatg gagtattcag gccaatcggg
     3721 gaggagcagg cccagcttat cctcccggcg gaggcccaag tcgatgagcg cctgggaaaa
     3781 agagtcgaca gctccctgga actcccggta ggtgtagctc tggtcctgga agaggatggc
     3841 cggacggtcg ggatgccggg ccgcggtctc tttcagcatc tgccccagcg tctttccctt
     3901 ggtccagttg attcccagaa cctggcagta gctctgcgtg agtctcctct tttccattgg
     3961 attcttctac cccttcttcc gtttttcctc ctggaccttt tgggccatga tctcccggag
     4021 gacgttcttc ttgatctttc cgctgggagt catggggaac tcggacatga gttccacccg
     4081 gtcgggaatc ttaaagtggg ctaccttgtc cttaaaaaag gcctttacct cctcctgggt
     4141 gatcggcccg cccggcttgg ggatgataca ggcgcaggtc ttctccccca ggcgttcatc
     4201 ggggatggcc actacgctca cctgggccac cttgggatag gtatagagga tgtcctcgat
     4261 ctccgtggga gagatgtttt cgcccccccg gacgatcatg tctttgatcc ggccgacgat
     4321 ccgcaggttt ccgttttcat ccatcgtggc caagtcgccg ctgtggatcc aaccccggtc
     4381 atcgatggtc tgggcagtgc gctccgggtc tttgaaatat ccgatgcaga cgttctgccc
     4441 gcgggcgcag gcctctccct ttacccccag gggaacgatg cgccgctttt catccaccac
     4501 cttgtgttcc attccgggtt ggggcctccc cacggtctcg cataccacct ccacggggtc
     4561 gtccaaacgg gtaatgttgc tcatggcggt ctcggtcatg ccataggcgg aagtcacata
     4621 gcaccccatc ttctggatga tttgccgggc cacttcgatg ggaagggggg cgccggccag
     4681 aaccatggct ctcaccgagg aaaaatcata ccgatcgaag tccggcctct ggagcatcat
     4741 gatgggcatg gtggggacgc cgacgggaag ggtcacccga ttgcgggtga tctctttcat
     4801 ggcctcctcg acgttccagg tctccaggag aacctggcag gagcctttga tgaccgaggc
     4861 ataaaaagcg ttgaccaggg ccgtggtgtg ggaaagggga accatgatca accagacgtc
     4921 gtcgggatgg agttgaatgg aggtggctgt ccccagggca gaccggcagc gggtattgtg
     4981 ggtgtgcatg acccctttag gattcccggt tgtccccgag gtgaagatga tctccagcag
     5041 atcatcgggc tccaccggga aatccttctg gtaaacttcc cgatagtaat gggggggata
     5101 gcgcttctcc cattcttggt ttaggaggtc ttccagggga atctcttcag gatgacgctt
     5161 ctgcccggag acgatcacgt gtttgagggt gggtatggct tcgcgtagct cctggaagag
     5221 gtcggaaaag gaaaatcccc agaactcaga gaccgaaacc gcagctttgg cttctgtttg
     5281 gcgaaggatg tactcgatct ctttttttct caaccgttcg gagatgggga cggcaatggc
     5341 tcccaaccgg gccgtggcca agagagagat gataaactcc ggccggttgg ggaaaagaat
     5401 ggcgatcatg tccccgcgcc ggacccccag atgaataaag gctaaagtca acctctccac
     5461 aaggagatgg agctctcccc aggtcaggcg ccggtcccga aagatcaggg ccacccggcc
     5521 tgaatgacga gcggcggtct gctcaaaata ttccccgagg ggcactcccg gaggaatctc
     5581 accgggagaa tagtaataac tcatcacggg cttttccatt ctccctcctc aaaatagtgt
     5641 gagtgtcagt gtaagtgtca gtcaaacttg ccactaccac tcacactcac actcacactg
     5701 acactgtatt tctcagctca tggtcaggcc cccggataca ctcagggtct ggccggtgat
     5761 gaatccggct tcgtctgagg ccagaaaagc cacggctccg gagatgtctt ccggtttggc
     5821 cagacgcctg aaaggaactg cattcttcat ggcttcgacg atcttggctc ccg
//
LOCUS       DASPRR010000374         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2181279, whole genome shotgun sequence.
ACCESSION   DASPRR010000374 DASPRR010000000
VERSION     DASPRR010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181279"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1361
                     /locus_tag="VEL68_13940"
     CDS             117..1361
                     /locus_tag="VEL68_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37116.1"
                     /translation="MKKALFSILWMAAAVSLVILNPSVSPAQGKGPIKIGYISPLSGM
                     MAANGKDMLGGIQLYLEEIGYQAAGRKIEFIVEDDEANPATGLTKTRKLVEKDGVHLM
                     TGGLMASTAYALAPYIDSKEIPMTYPIMSADDITQRKIPKWIVRTGWSSSQPHQPFGE
                     YAYNVLKFKKIAVVAYDFAFGWECVGGFHKAFEDAGGRIIQKIWVPLTAQDMSPYLSQ
                     ISKDADAVFAVFSGRQAMQFIKQYQEFGLKGKIPLIGGGTTTDEHALPSMGDEAIGVI
                     TPLHYSEALDNPANRKFVKAFREKAKKAASYYSEGTYTGARWIVEAIKAINGDVENRG
                     KLLEALKKVELKDVPRGPMKLDSYGNPVQNIYIRKVERVGGELQNTVIQTYPEVSQFW
                     KYKPEEFLKQPVYSREYPPLKP"
     gene            1471..2328
                     /locus_tag="VEL68_13945"
     CDS             1471..2328
                     /locus_tag="VEL68_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37117.1"
                     /translation="MSEFLLVQTLNGISFGALLFLLASGLSLIYGVMKIVNIAHGSYF
                     MLGAYVGLSVILRTKSFILGALAGGLAVGALGLVMERGFLRKFPLQALPQMMITLGFA
                     LLFRDLALLIWGGDPYTLPCPEFLKGSTQIYKVVFPVYRLFVIGVAAAVAVSLWLFND
                     KTLVGAKLRAAVDDHEMAGGVGLNVPLISGCMFGLGAFLAAFGGVMGGPIFGVYPGLD
                     FELLSLAFVVVIVGGRGSLKGSAVGSILVGLVDNFGKALVPELSYFTLFAPMAIVVAI
                     KPTGLFGKE"
     gene            2549..3550
                     /locus_tag="VEL68_13950"
     CDS             2549..3550
                     /locus_tag="VEL68_13950"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37118.1"
                     /translation="MKNLWATKAKIAGLLLAFLVLAVLPPFYSSYWVTLFTQMLIFAI
                     LAMSLDVLLGYTGLSSFGHAAFFGAGAYAVAILSTRYKMGFLVCFLSGIALSTGISSI
                     FGLLVAHATGVYFLIITLALGMTLWGLAFRWVNMTGGDNGISGIGRPDIGLPLSLKDP
                     LTFYYVIFVFFLIGLILMAILVRSPFGHSLKGVRESESRMRVLGYHTWLHKYLSYICS
                     ATFGGFAGVLWAYFNGFTSPYDMDLTASIEIILMVILGGPGTLIGPALGAGIIVFLKN
                     FISAYTQRWLLILGTIYILTILYAPQGLVNFIKEYLKRGQKEARETKDDWRNGTSVV"
BASE COUNT          768 a          939 c         1019 g          866 t
ORIGIN      
        1 caaacccgag gagtttctga aacaacccgt ctattcgagg gagtatcctc cgttgaagcc
       61 gtgaacgggg gaggagattg agtcagcacc caaatcagag gaggaggatt gggaccatga
      121 aaaaagcatt attcagtatt ctctggatgg cggcggcggt gagtttggtc attttgaatc
      181 cttcggtttc accggctcag gggaagggcc cgatcaagat cgggtacatt tcgcctcttt
      241 cgggcatgat ggcggccaac ggaaaagaca tgctgggggg gattcagctc tatcttgaag
      301 agatcgggta ccaggccgcc ggccggaaga tcgagttcat tgtggaggac gacgaggcga
      361 acccggcgac cggtctgacc aagacccgaa aactggtaga gaaggacggg gttcacctca
      421 tgaccggggg actcatggct tcgacggcct acgcccttgc gccctacatc gactccaaag
      481 agatccccat gacctacccg atcatgtcgg cggatgatat tacccaaaga aaaattccca
      541 aatggatcgt caggacgggg tggtctagca gccagcccca tcagcctttc ggcgagtacg
      601 catataatgt tctcaaattc aagaagatcg ccgtagtcgc ctatgatttc gccttcggct
      661 gggagtgcgt gggggggttc cataaggcat ttgaggatgc cgggggccgc atcattcaga
      721 agatctgggt tcctttgacc gcccaggaca tgagccccta tctctcccag atttccaagg
      781 acgccgatgc cgttttcgcg gtcttttccg gccggcaggc gatgcagttc atcaagcagt
      841 atcaggagtt cggcctaaaa gggaagattc ctctcatcgg cggaggaacg accacggacg
      901 aacatgccct cccctccatg ggtgacgaag ccatcggagt cattaccccg ctgcactaca
      961 gcgaggcctt ggacaacccg gccaacagga aatttgtcaa agcctttcga gagaaggcca
     1021 aaaaggccgc atcttattat tccgaaggga cctataccgg agcccgatgg attgtggagg
     1081 cgatcaaagc tatcaatggg gacgtcgaaa atcgcgggaa gctcctggag gctttgaaaa
     1141 aggtggagct caaggacgtt ccccgggggc ccatgaaatt agacagctac ggaaatccgg
     1201 ttcagaatat ctatatccgg aaggtagaaa gagtgggggg agagcttcag aacacggtga
     1261 ttcaaaccta tccggaggtg tcccagttct ggaaatacaa accggaggaa tttctcaaac
     1321 agccggtcta ttcccgggaa tatcctcctt tgaaacctta agcggccata gccgggtaga
     1381 gaacggcata gctttccgta ccgcttgtag gggcgattct cgtagagtcg agtctccgac
     1441 atgaatcgcc cctacgaagg aagaaaaatc ttgagcgaat ttctactggt ccaaaccctg
     1501 aacgggattt ctttcggggc ccttcttttc ctgctggcca gcgggctatc cctcatctac
     1561 ggggtgatga agatcgtgaa catcgcccac gggtcctatt tcatgctggg cgcctacgtg
     1621 gggctttcgg tcatcctgcg gaccaaaagc tttatcctag gagccctggc cggaggcctg
     1681 gccgtcggag cgctcggtct agtcatggag cgcgggttct tgagaaagtt tcccctacag
     1741 gcccttcccc aaatgatgat caccctgggg tttgccctgc tcttccgcga tttggccctc
     1801 ctgatctggg ggggcgatcc ctataccctt ccctgcccgg aattcctgaa agggtcgacc
     1861 caaatctaca aagtcgtgtt tcccgtctac cgccttttcg tcatcggggt ggctgccgcg
     1921 gttgcggttt ccctttggct cttcaatgat aagacattgg tgggggccaa attgcgggcg
     1981 gcggtggatg atcatgaaat ggcgggaggg gtgggcctca acgttccctt gatttcgggg
     2041 tgcatgttcg gtttgggagc ttttctggcc gccttcggag gggtcatggg gggccctatc
     2101 ttcggagtgt atcctggcct ggattttgag ctcctctctc tggctttcgt cgttgtgatt
     2161 gtgggcggaa gaggaagctt gaaggggtct gccgtgggta gtatcctggt tggtttggtt
     2221 gacaacttcg gaaaggcgct ggtcccggaa ctctcctatt tcaccctctt cgcccccatg
     2281 gcgatcgtgg tggccatcaa gcccaccggg ctcttcggga aggagtagga caccaattcg
     2341 gtagcttgaa ataatggctt ttcatcggag ttcatggatg aaattccaat taccaaatcc
     2401 caaattccaa ataatatcca agctccaatt gacgaaccct tttgaatttt ggtcattttg
     2461 actttggata ttatttgaga tttggtgctt ggcgtttggt gcttgaaaat tcgtgaagat
     2521 ctttccaagg tcagaataag cacagaaaat gaaaaacctc tgggcaacga aagctaaaat
     2581 cgccggcctc ctcctggcgt tccttgtcct ggcggtcctg cccccatttt acagctccta
     2641 ctgggtcacc ctcttcaccc agatgctgat ttttgccatc ctggccatga gcctggatgt
     2701 cctcctggga tacacaggtt tgtcatcctt cggccacgcg gcttttttcg gagcgggagc
     2761 ttacgcggtg gccattctct ccacccgcta caagatgggt ttcctggtct gcttcctcag
     2821 cgggattgct ctgtccacgg ggatcagctc gatcttcggt ctcctggtcg cccacgccac
     2881 cggggtgtat tttctgatca tcaccttagc cctgggaatg accctctggg gtctggcctt
     2941 tcgctgggtc aacatgaccg gaggagacaa cgggatctcc ggaatcgggc gacccgatat
     3001 tggattgccc ctctcgttga aggatcccct caccttctat tacgtcatct tcgttttctt
     3061 cctgatcggc ctgatcctga tggccattct ggtccgctcc cctttcggcc acagcctgaa
     3121 gggggttcgc gagagcgagt cccgcatgcg ggtcctgggg tatcacacct ggctgcataa
     3181 atacttgagt tatatttgtt cggcaacctt cgggggcttc gcgggggtcc tgtgggccta
     3241 tttcaacggg ttcacgagtc cttacgacat ggacttgacc gcctcgatcg aaatcattct
     3301 gatggtcatc ttggggggcc cgggaacctt aatcggccct gctctggggg cggggattat
     3361 cgtattcctt aagaacttta tcagcgccta cacccagcgc tggctcctca ttctggggac
     3421 catctatatc ctcaccattc tctacgctcc ccaggggttg gtgaacttca tcaaagaata
     3481 tctgaaacgg ggtcaaaagg aagcgaggga gacgaaggat gattggagaa atggaacgtc
     3541 agtagtatga aggaagaggt tgagtagatg ggatcaaaag aaggcacggg gg
//
LOCUS       DASPRR010000375         4716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2340448, whole genome shotgun sequence.
ACCESSION   DASPRR010000375 DASPRR010000000
VERSION     DASPRR010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340448"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VEL68_13955"
     CDS             complement(<1..188)
                     /locus_tag="VEL68_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37119.1"
                     /translation="MKYSNFMSIPLRKAFGLCRRISRGKTFHAILEMGKMVAIIATAF
                     FLSQGYFFPSAQSSQTET"
     gene            976..2241
                     /locus_tag="VEL68_13960"
     CDS             976..2241
                     /locus_tag="VEL68_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37120.1"
                     /translation="MPKKRRLSRGVAVVGGGLSKLGLFKDRNSKDFFAEAYLEMLASV
                     DKGVDPKDIEAIYIGNFTNDFFVNQAHWGPIISDLVGHVPKPVTRTEGACASSALAFR
                     EGVFAVASGFYDMVLVGGLEQMSKRTTEEVAEGLGTAGSPYEVAAGFTFPGVFGTIAT
                     AYFAKYKTNRETLFNVTIKSHLNAPLNPKAQFPLTIRDFMNTRVEKCRKRGEPVPDWK
                     DEKDFLRDLSVNPPVSWPMHLFDCCPISDGASCMLLVAEDLAKRFTDEPVYVAGIGQG
                     SGYSLHATQNITSFEATRAAAQEAYGMSGLKPEDIQFSEVHDCFSIAEIIHTEDLGFF
                     KPGEGHKAAAEGLTRLEGPKPINTSGGLKSKGHPVGATGVAQLYEVWLQLRGKAGKRQ
                     VPKKNLRVGGAHNLGGTGGTCTFTILERR"
     gene            2245..2658
                     /locus_tag="VEL68_13965"
     CDS             2245..2658
                     /locus_tag="VEL68_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ37121.1"
                     /translation="MYDRPFNDNSYEQFLKEEKLMGSRCKKCGALALPPRPICVSCFG
                     SEMEWVQFKGAGKLAAFTSIVVAPPFMAKEGFNKNNPYVVGVVELEEGTKIVGRITGV
                     EAKKPEGIQVGAPLKAEFIRSGQGPEQKTYLAFRL"
     gene            2793..4259
                     /locus_tag="VEL68_13970"
     CDS             2793..4259
                     /locus_tag="VEL68_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXZ37122.1"
                     /translation="MEKRKRTETLEEWELCYLPALKLRDLIRKKIISPVEVVRTFLQR
                     IEKVNPIINAYCTVVPELTLKAAKRAETAIMKKTPVNPLHGVPVSIKDVTLTTGIRTT
                     FGSKLYENFIPNQDALVVERLKKAGAIILGKTNTPEFAAGGSTFNKVFGITRNPWNPD
                     FNSGGSSGGAAAAIAAGLGPLAQGNDLGGSLRIPASFCGVVGLRPSPGRVPSVPNELC
                     WDPFNVEGPMARTIGDLALMLGVMSGPDDRSPISLPEEKKGFLGSVKNPSIKGFRVAW
                     GGNLNLTPVDHEVLEIAQAAAKMFLRLGCRVEAKAPDFEGVRETALIYRGLRYVSLYQ
                     DRLEDPEFKSLVNPLVAGNVEQGLKFSIRDLARAERQRSDLWQRAQAFFEEYDLLLTP
                     TVPIPAFPAQTVFPTEINGKAMENYIDWVMLTYAFSVLGLPAISVPCGWTQNNLPVGL
                     QIIARRQGEAVLLKAAAAFENAAPWADRRPSLPGFGQG"
BASE COUNT         1132 a         1221 c         1293 g         1070 t
ORIGIN      
        1 aaggtctctg tttgactgga ttgggcggag gggaaaaaat atccctggga tagaaaaaaa
       61 gcagtggcaa tgatggcaac cattttgccc atttccagga tggcatgaaa agtcttccct
      121 cgagagattc tccggcacaa gccgaaagcc ttgcgcaggg ggatagacat aaagttggaa
      181 tatttcataa ctttctattc tcggcctaat ttcctccccg gcccagggta agttcaggaa
      241 gccgggaatg cctcttctaa ggcttcgatc aagtcaatca cggattctat gatgctccgc
      301 ttcctgccaa atttatgggg aaggggagat tttcttcaag agaactcgtc tataaaaagg
      361 cccttaggct gccgaatttc caaattaccc tgtcgatcat agttggtggc cttctcaccg
      421 ttctgcaagt tcaatccggt ctcttcgtat gaaacttcga ttactggctc aaccctttgc
      481 ggcaagagcc tttgataaga aatgattccc cctgttgggg gaaggcatat gggccccacg
      541 attgaataga ccatagcccg aagaaagaca atttttttta ttttttgccc cctcttctcc
      601 ccagggaaga ggggcgatcg attcaagagt ttttttaaat gcccttttga tatcaggaaa
      661 agccagatcc atgccaggct aaccccagcc aagtcggttt cttaaatttc gaattttaca
      721 cagcaatcaa acaccaagaa aaaattaccg ggaaagaata cgagattgcg ggggagaggg
      781 tatggatctt aacccataaa tttatggagc cttacccata tcctcttgcc cttcgaaggg
      841 ccggccctca aactctttcg agaggaaacc cctcaagaga gaatggttga ttctgcctta
      901 aagaataggg cataatatca aaaaactcgg gaatccgaaa tttgcagggc ccattccatt
      961 cgcctggagg tcgatatgcc caagaagaga agactttcga gaggggtagc ggttgtcggg
     1021 ggcggtctgt ccaaactggg tttgttcaaa gaccgcaatt ccaaggattt ctttgctgaa
     1081 gcctacctgg agatgcttgc ctcggtggac aaaggggtcg accccaaaga catcgaggcg
     1141 atctacatcg gaaacttcac caatgatttc ttcgtgaatc aggcccattg gggtcccatc
     1201 atctccgatc tggtggggca tgttcccaag ccggtgaccc gtacggaggg agcctgcgcg
     1261 tcgagcgccc tggctttccg ggagggagta ttcgccgtgg cctcgggatt ttacgatatg
     1321 gtcctggtgg gggggctgga gcagatgtcc aaacggacga cagaggaagt ggccgaggga
     1381 ttgggaacgg ctgggagtcc ctatgaggtg gccgccggct tcacctttcc cggggttttc
     1441 ggcacgattg ccacggccta tttcgccaaa tacaaaacga accgggagac gctcttcaac
     1501 gtgaccatca agagccatct caatgctccc ctcaatccca aagcccaatt tcccctgacc
     1561 atccgcgatt tcatgaatac gcgggtcgag aaatgccgca aaagggggga acccgtgcct
     1621 gattggaagg atgaaaagga ttttctccgg gacctttcgg tgaaccctcc tgtctcctgg
     1681 cccatgcatc ttttcgattg ctgtcccatc agcgacgggg cctcctgcat gctcctggta
     1741 gccgaggatt tggccaagcg gttcaccgat gagccggtct acgtggcagg catcgggcag
     1801 ggaagcgggt acagcctaca tgccactcaa aacattacct ccttcgaggc cacccgggcc
     1861 gcggcccagg aagcctacgg aatgtccggt ttgaaaccag aagacattca gttttccgag
     1921 gtccacgact gtttttcgat tgcggagatc atccataccg aggacctggg tttcttcaaa
     1981 cccggggaag gacacaaggc cgcggctgaa ggtctcaccc ggctggaagg gccaaagccg
     2041 atcaacacct cagggggcct aaaatcaaag ggacatcccg tcggggccac cggagtcgcc
     2101 cagctttatg aggtgtggct ccagctaagg gggaaagccg gaaagcgcca ggtacccaag
     2161 aagaatttga gggtcggtgg ggcccataac ctagggggca cgggcggcac ctgcaccttc
     2221 accattctgg agaggaggtg acccatgtac gaccggccgt tcaatgacaa ctcctacgag
     2281 cagtttctga aggaagaaaa gctcatgggt tcgcggtgca agaaatgcgg tgccttggcc
     2341 ctgcccccgc gtccgatctg cgtctcctgc ttcggcagtg aaatggaatg ggtccagttc
     2401 aaaggagcgg ggaagctggc cgccttcacc agcatcgtcg tagccccgcc cttcatggcc
     2461 aaggagggat tcaataaaaa caatccctac gtggtgggag tggtggaact ggaagaagga
     2521 accaagatcg tggggcggat cacgggggtg gaggcgaaaa aacccgaagg aatccaggtg
     2581 ggcgcccccc tgaaggcaga attcatccgc agcggccagg gacccgagca gaagacttat
     2641 ttggctttcc gcctttgact ctatccctct accgggcagg tgggttctga cccccacctg
     2701 cctgcctcca agttcgtttt tttacaaatc aaaagggttt ccccctacaa tttaggccat
     2761 cttcataaat atcctgaaaa ggaggattgg ctatggaaaa gcggaaaagg acagaaaccc
     2821 tggaggaatg ggagctctgt taccttcccg ccctgaagct cagggacctg atccggaaaa
     2881 agatcatctc ccccgttgag gttgtccgga cctttctcca gcggatcgag aaggtgaatc
     2941 ccatcattaa tgcttattgt accgttgttc cagaattaac attgaaggcg gcgaaaaggg
     3001 ctgagacagc gatcatgaag aaaaccccgg ttaaccccct ccatggagtc ccagtctcca
     3061 ttaaagacgt gactttaacc accgggatcc ggaccacttt tgggtcgaag ttatacgaaa
     3121 attttattcc caaccaggat gccttggtgg tggaaagatt gaaaaaggcc ggagcgatta
     3181 ttctgggaaa gacaaatacc cccgaatttg ccgctggagg gtctactttc aataaagtgt
     3241 ttgggatcac ccggaacccc tggaacccag attttaacag cggcgggtcc agcggagggg
     3301 cagcggcagc cattgccgcc gggttaggtc ccctggccca aggaaacgat ttgggaggat
     3361 ccctccgcat ccccgccagc ttctgcggtg tggttggatt gcgaccttct cccggccggg
     3421 tgccctccgt gccgaatgaa ctctgttggg atccgtttaa cgtcgaaggg cccatggccc
     3481 gcaccattgg ggatctcgct ttgatgctgg gggtcatgtc cggcccagat gatcgcagcc
     3541 cgatttcctt gccggaggaa aaaaaaggat ttttggggtc ggtgaaaaat ccttccataa
     3601 aaggcttcag ggtggcctgg ggagggaatt tgaacttgac tcccgtggat catgaggttc
     3661 tggaaatcgc ccaagcggcc gccaagatgt tcctccggct gggatgcagg gtggaagcga
     3721 aggcgcccga tttcgagggg gtgagagaaa cagccctcat ctaccgggga ttgcggtacg
     3781 tatccttata ccaagatcgg ctggaggacc cggaattcaa aagtctggtc aatcctctgg
     3841 tggccgggaa tgtggaacaa gggctgaaat tttccatccg cgatctggcc cgggcggagc
     3901 ggcaaaggtc tgatctatgg cagcgtgctc aggcattctt cgaggaatat gatttgctct
     3961 tgactccaac cgtgcccatt ccggcctttc ccgcgcaaac ggtctttccc acggaaatca
     4021 acgggaaggc catggagaat tatatcgatt gggtcatgct gacctacgct ttcagcgtgc
     4081 tgggtttgcc ggccatatcg gtcccgtgcg ggtggaccca aaacaatctg ccggtcgggt
     4141 tgcaaatcat tgcccggcgt cagggggagg ccgttctatt aaaagccgca gcagcctttg
     4201 agaatgcagc cccgtgggct gacaggcgcc cttctctccc tggttttggc cagggatgaa
     4261 aagaactgtc ctttgagttc taaagaattt tcttgacatt tttttcttag atggactatg
     4321 aaattggcag attgattatt ttttgaccat atcccattgc cgacgatccc tttagctggg
     4381 tcaaaaacgg aaaccttgta gagagtggcc cggctcgttt gttcgctttg cgcaagtcga
     4441 tttcaaggcc gaagaggaac gacatgcgcg gaaagaacga tcttcaggtt cagcctttaa
     4501 aggacgcctc attcaatccg tccctcaccg tataccctcc caaaagagtg atccggtttg
     4561 taggcgaagt tacgacatat ttcctgcttt ttatgtttcc cacaggggac cggagctcat
     4621 ccccaaattc tttccgagcc atatccaaga aattcaacct ctctggccag atgccagaaa
     4681 tggatgaagc tggggcacaa cctttaacct tttcgg
//
LOCUS       DASPRR010000376         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2182006, whole genome shotgun sequence.
ACCESSION   DASPRR010000376 DASPRR010000000
VERSION     DASPRR010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182006"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VEL68_13975"
     CDS             complement(<1..784)
                     /locus_tag="VEL68_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HXZ37123.1"
                     /translation="MSVQAQLPVVKLIATGGTIAMKIDPVKKAPVPAITGEDLISTVP
                     EIAQVAKMEVQNISNVPSGYMDPPRWIELQKAVAEALSRPEVAGVIVSHGTDTLEETA
                     YFLDLTVASEKPIVLIGAQRNASEKDFDGPRNLYNAARICVSPDAKAKGAMIVLNNQI
                     NAAREVTKTHTSDVETFKSGDYGFLGIADADRIIFYRSPLRRQHIPIKMEKLPYVEII
                     PAYGGADGTLIRAAVAAGAKGLVIQALGWGNVNIPQYEAIKEV"
     gene            complement(1283..>2319)
                     /locus_tag="VEL68_13980"
     CDS             complement(1283..>2319)
                     /locus_tag="VEL68_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ37124.1"
                     /translation="FKMQEAEVLAQYFKLYKKAKAGADFVITQVGFDARKFEEALRFM
                     RQQGIQVPIIGNVYVLNRPVARVMNKGDVPGCVVTDTLYRIIEQESKAPDKGKTARLT
                     RAAKLIAVLKGIGYQGVHIGGPNIKYEDVEWVIGKSKEFSANWQDWVREFSFPQENGF
                     YLFMEDSKTGLNRDMPNFKRIHPRKKWGYRMMRFVHHFIFLSGAPLFKTACWFFRNLD
                     GSRLEIPFTEMEYWIKFASSRCQRCGDCTLSELAFLCPQSQCAKFLLNGQCGGSREGW
                     CEVFPGKKKCIYVRAYQRLKAYAEEESMMDGYIPPRNWGLTGTSSWANYFLSRDHHAV
                     NQGSNRSTLA"
BASE COUNT          506 a          616 c          609 g          588 t
ORIGIN      
        1 taacctcttt gatcgcctca tactggggga tgttcacgtt tccccatccc agcgcctgaa
       61 tgacgaggcc cttggccccc gccgccaccg cggctcgaat gagcgtccca tcggctcctc
      121 cgtaagcggg aataatttct acgtagggga gcttttccat cttgatgggg atatgttggc
      181 gccggagagg agaccggtag aagatgatcc ggtctgcgtc ggcgatcccc agaaatccgt
      241 aatcccctga cttgaaagtc tccacatcgg aggtgtgggt tttggtgacc tcgcgggccg
      301 cattgatctg gttgttcagc acgatcatgg cccctttggc cttagcatcg ggggatacgc
      361 agatacgggc ggcattgtat aagttccggg gaccatcaaa atccttttcc gaggcgtttc
      421 gctgggcgcc gatgaggaca atcggctttt cactggccac cgtgaggtct aggaagtaag
      481 cggtctcttc cagggtgtcc gtaccgtggg aaacgatgac tccggccacc tcgggtcggg
      541 acaaagcctc ggctacagcc ttttgcagct caatccaccg gggcgggtcc atgtagcccg
      601 aagggacatt ggagatgttc tggacctcca ttttggccac ctgggcaatt tccgggacgg
      661 tggaaatcag gtcttccccc gtgatggctg gaaccggggc tttcttaacc ggatcgattt
      721 tcatggcaat ggtcccccca gtggcaatga gtttgaccac cgggagttgc gcttgcacgc
      781 tcatggggag aagaagcaac gcgatgcagc cagcaacaat caacagatgg aatttttggg
      841 tccgcagact attcatactc tctcctttcc tttttggtgt tttgggatgc tcagcgggtt
      901 ctccaaccct gccgaggaaa attgataagg agctttgaac catcgacttt cgcaggcatg
      961 gaccattccg cgaggttgac cttgattcca gcattcctga cttgccggaa attactccga
     1021 tcagcccctt tgaccgccaa gagatggatt atgttccgcc ccaattttat tatattgaag
     1081 ggataagaaa atagggtaaa gactgtattt tttgaccgga aacgctttat attcaaacaa
     1141 tttgatatga ttatagccat gattttcttt tgatatttct gtagggcgtg atccctgatg
     1201 acgcctttcc gcgatgcgtt cggcgaacgc accctatatt ctttctttgt attcctattt
     1261 atcgcgttag gatcagctgc cgctaagcga gtgtcgagcg attcgacccc tgatttaccg
     1321 catgatggtc acggctcaaa aagtaattgg cccaggatga agttccggtc aggccccagt
     1381 tacggggggg aatgtatcca tccatcatgg attcttcctc cgcataggcc ttcaggcgct
     1441 ggtaggcgcg gacgtagatg cacttcttct tgccaggaaa cacctcacac cacccttccc
     1501 tggacccgcc gcattgtcca ttcaaaagga atttggcgca ttgggattgg gggcaaagaa
     1561 atgccagttc ggagagggta caatccccgc accgctggca gcgggaagag gcaaacttga
     1621 tccagtactc catctcggta aacgggattt caaggcggct tccgtcaagg ttgcggaaga
     1681 accagcaggc cgttttgaag agcggagcgc ccgataaaaa aataaagtga tggacgaaac
     1741 gcatcatccg gtacccccat tttttgcggg ggtggatgcg cttgaagttg ggcatatctc
     1801 tgttgaggcc ggtctttgaa tcctccatga agagatagaa gccgttttcc tggggaaagg
     1861 aaaattcccg gacccaatcc tgccagttgg cggaaaattc ttttgatttt ccaataaccc
     1921 attccacgtc ttcgtattta atattgggtc ccccgatatg gaccccctgg tacccgatcc
     1981 cctttagaac ggcaatcagc ttggccgccc gggtaagacg ggcggttttt cctttgtccg
     2041 gagccttgga ttcttgttcg ataatccgat acaaagtatc ggtcaccacg catcccggta
     2101 catctccctt gttcatcact ctggccaccg gccgatttaa gacatacaca tttccgatga
     2161 tggggacctg gatgccctgc tgacgcatga accgcagtgc ttcttcaaac ttccgggcat
     2221 cgaatccaac ctgggtgatg acgaaatccg cccccgcttt tgccttctta tacagcttga
     2281 aatattgggc caggacctct gcttcctgca tcttgaagg
//
LOCUS       DASPRR010000377         4794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2025164, whole genome shotgun sequence.
ACCESSION   DASPRR010000377 DASPRR010000000
VERSION     DASPRR010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025164"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VEL68_13985"
     CDS             complement(<1..203)
                     /locus_tag="VEL68_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37125.1"
                     /translation="MTKNFSIFFSILAFGVCSCAAPVVSQMPKTDHDLALDACIFLGN
                     YSPSSAGYVLSTREHFLNAPSPF"
     gene            complement(390..665)
                     /locus_tag="VEL68_13990"
     CDS             complement(390..665)
                     /locus_tag="VEL68_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37126.1"
                     /translation="MNRIIRFLALPALVPIFFFAVASTPVELLGCRNRGLLAVMIALA
                     GALAALGAAIMGTKGKIQRDPQAHWWAISAVVLAIPAVAIILIAYRE"
     gene            complement(961..2049)
                     /locus_tag="VEL68_13995"
     CDS             complement(961..2049)
                     /locus_tag="VEL68_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018783761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HXZ37127.1"
                     /translation="MAASRIVVLPGDGVGPEIIAEGLKVIRVAMKAVSLEAQFTELEI
                     GAARYRRTGTPFGQEDIEKVRKADAVYFGAVGLPDVRLPDGREVQSGYIYRQDLDLYA
                     NIRPVKLYKGVESPLKRAQEKGIDYVIFRENTEGLYTFGKGGFRIGKEAAVNPLIISR
                     KGTERIVRAAFQMARRRKGAPDDGVKRVTCVDKSNVVEAYAFFEEVFHDVAKEFPDIQ
                     SEHYYTDAVTVLMVQRPWHFDVVVTENMFGDILSDLGAGTIGSLGLAPSAEVGDKHGL
                     FQSIHGSAPDIAGKGIVNPIAAILSGAMMFDWLGMKHKDPKATEVGRRIEKAVEAVLA
                     KGQVRTPDLGGKNTTSQMGDEIVKEIEK"
     gene            complement(2163..3080)
                     /locus_tag="VEL68_14000"
     CDS             complement(2163..3080)
                     /locus_tag="VEL68_14000"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXZ37128.1"
                     /translation="MSRAVRRSTLILPVNIPKFVEKAYQRGADAILLDLEDAVPWAEK
                     DNARKLVKDSIALAAKGGAEVGVRINKDPKWLSKDVEASVHPGLDGITLPKAESAEEV
                     RNLEKMVEKFEREKGIKPGHIQFSILIETPRGLLKAQEIATASPRIESMSIGPEDYCL
                     ELGVEPSPDGSEIFYAVSWIVTVCKPVGVRPMGMLGSIGGFRDLEAFEKAVSRARQLG
                     CEGASCIHPDQVPVINGVYTPTEDKVAFARRAVEVFEEGVKKGTASVNLDGKMVDIPV
                     YNRAKLILARAEAIAAVEKRKAAALAKMK"
     gene            3608..>4794
                     /gene="larA"
                     /locus_tag="VEL68_14005"
     CDS             3608..>4794
                     /gene="larA"
                     /locus_tag="VEL68_14005"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HXZ37129.1"
                     /translation="MRKAAIPWGCWRGSKSLPLTFPNNWKIAMAPMAGKPDLSQEEIR
                     KAFQNPIGQEPIRKLAEGKKTAAIAVDDLTRPTQAHRFLPFIVEELNQAGIQDEDIKI
                     IMAVGCHRPLMKADQEKKLGKKMANRFAVYNHHPFENVVNVGTTSRGTPVIINRYFVE
                     SDVKIGIGFITPHPIAAFGGGGKIVIPGLGSIDTIHRNHTPAFQGKVGSTGFSKGYDI
                     NSNELRADIEEGARLAGLNAIVNSVGTSEGKTAGVFVGDLVQAHRAAVDLARTVYRTD
                     APCDMDVGIFNAFPEDTELVQAQKAMNVWTGNETCRLVREGGKVVIATASSDGLGFHS
                     LADRGMRLYNRVGHRKAMADILSGRKVIVFSPNCSRADLLERYPEEVIIRNTWNDVLE
                     ELK"
BASE COUNT         1075 a         1380 c         1247 g         1092 t
ORIGIN      
        1 aagaaggggc tgggggcatt caagaaatgt tcacgcgtac tcaggacata tccggccgag
       61 ctgggactat aattgcccag aaagatacag gcatccaagg ccaggtcatg atcggttttc
      121 ggcatttgag aaacaacggg cgccgcgcag ctgcagacac caaaggccaa aatactgaaa
      181 aaaatcgaga agttttttgt catatctact ccttaaagaa ccgccggttt tgagtattgc
      241 cggaaggaat ttactctttt cgtttccaca agttaaccgc ttctttctga aggaagcctt
      301 tctttcacgg aaattattaa tagggtctaa tccggcaatc aaaattccct tgataggatc
      361 ccctcatcat aagggacggc caaagctgat cactcccggt aagcaattaa tataatggca
      421 accgccggga tggccaggac tactgcgctt atagcccacc agtgcgcctg tggatctcgc
      481 tgtatttttc cctttgtccc cataatcgcc gcacccagag cggccaaagc tcctgcaagc
      541 gcgatcatga ccgcgaggag cccacggttg cgacaaccca gcagttcaac cggcgtggaa
      601 gcaacggcga aaaagaagat ggggacgagc gcgggcaagg ccaggaaacg gatgattcgg
      661 ttcatgcctg attccttgcg attttctcct aagctcttct gatttttcgg cgaaaatgga
      721 gaatttcttt aaatggattg gatcggggag aatgggcggc ttttgcctat ttcagctccg
      781 gcaagacggc cgccccggaa gaaaggcagc ggctggggcc gctgcctggc gggaagagaa
      841 tctttcctgt gaatgtagga cattaaccct gacgaataat ctttgtagat catggcctta
      901 gccctgactc cccatggcag acgttcattt cataagccca atggattgag ttacggctgg
      961 ctatttttct atctccttaa caatctcatc ccccatctga gacgtggtgt ttttcccgcc
     1021 caagtcggga gttctcacct ggcctttggc caggacggct tccactgctt tttcgatcct
     1081 cctccctact tccgtcgctt tggggtcttt atgcttcatt cccagccagt caaacatcat
     1141 ggcgccggag aggatggcgg cgatcgggtt cacgatgccc ttgccggcga tgtccggggc
     1201 gcttccatgg atggactgga agaggccgtg cttgtctccc acttcagcag agggcgccag
     1261 gcccaggctt ccgatggttc ccgcccccag gtcggaaaga atatctccga acatgttctc
     1321 ggtcaccacc acgtcgaagt gccagggcct ctggaccatc aggacggtca cggcatccgt
     1381 gtagtaatgt tcgctctgga tgtcggggaa ctccttggct acgtcgtgga agacttcttc
     1441 aaagaaggca taggcttcca ccacattcga cttgtccacg caggttaccc gcttgacgcc
     1501 atcgtcggga gcccccttgc gccgacgggc catctggaag gcggccctca cgatccgctc
     1561 tgttcccttc cgggaaataa tcaggggatt caccgccgcc tctttcccga tccggaatcc
     1621 tcctttaccg aaggtataaa ggccttccgt gttctcccgg aagatgacat aatcgattcc
     1681 tttctcctgg gccctcttca gcggggattc aacccctttg tatagtttca ccggccggat
     1741 gttggcataa aggtcgaggt cctgtcggta gatatatccc gactggacct ctctcccatc
     1801 gggcaggcgg acatcgggaa ggcccacggc gccgaagtaa accgcatccg ctttccgaac
     1861 cttttcaatg tcctcctgcc caaaaggtgt gcccgtcctc cgataacggg ccgcaccgat
     1921 ctccagttcg gtgaattggg cctccaggga aactgctttc atggccaccc gaatcacttt
     1981 caatccttca gcaataatct cggggcctac tccgtccccg ggcaatacaa cgattcgaga
     2041 tgctgccatt ccttcctcct tgcgtgggat agaaagtctc gagtctcgat tttccggttt
     2101 cgagttttcg gtcttaacgc ttcggtttta aactcgaaac ctgcaaccca taacccgtaa
     2161 cattatttca tcttggccag cgccgctgct ttcctctttt ccactgcggc gatggcttcg
     2221 gcccgggcca ggatcagttt cgcccgattg tacaccggga tgtccaccat cttcccatcc
     2281 aggttgaccg aagcggttcc cttcttcacc ccttcttcaa agacctcaac agccctgcgg
     2341 gcaaacgcca ctttatcctc ggtgggggtg taaaccccgt tgatcaccgg aacctgatcg
     2401 ggatggatgc aggaagcccc ttcgcagccg agttgccggg cccgcgacac cgccttctca
     2461 aaggcttcca ggtcccggaa tccgccgatg ctccccagca ttcccatggg ccggaccccg
     2521 accggtttgc agacggttac gatccaggag acggcgtaga agatctcgct tccatccggc
     2581 gacggctcaa cgcccaattc caggcaatag tcttccggcc cgatgctcat ggactcaatc
     2641 cgcgggctgg cggtggcgat ctcctgtgct ttcagcaatc cccggggagt ttcgatcaga
     2701 atggaaaatt ggatgtgccc cggtttgatt cccttctccc gttcgaattt ttccaccatc
     2761 ttctccaggt tgcgcacttc ttccgccgat tcggccttgg gcagggtgat cccatccagc
     2821 cccgggtgaa ccgaggcctc tacgtcttta gaaagccact tcggatcctt gttgatcctc
     2881 acccccacct cggcaccgcc tttggcggcc aaagcaatcg aatctttgac cagcttgcgg
     2941 gcgttgtctt tttccgccca gggaaccgca tcctccaggt ctaataaaat cgcgtccgct
     3001 ccccgctggt aggccttctc cacaaacttg gggatgttca ccggcaggat caaggttgaa
     3061 cgtcttacgg ctctactcat ttgcagtctc ctttaatcaa gttgttgctt caaggctctt
     3121 tatattgaaa aacagcctga ctcccagatt acaatctatt tctccaaatt acaataatta
     3181 caatccttgc ccacccccag ttcttgaaaa cgagtccttc gaaaataaga tctctggaaa
     3241 ggagggggga tctcatgggg ttcagggcag agaaatttgg atcatcatcc tgaggcggtg
     3301 attcttccat tggatcaggc ctgtcctggg gggattgaaa cggaaaaatg ctttggttct
     3361 cttttctgct tggaggaatc tcctctcagg cctctccctg cggaacatgg ggaggagttt
     3421 catggaagga taagcacccg ccgagcctga tgatccaaat tcctccaccc tgaaccccaa
     3481 caagcacgaa aaactggggg tgggcaagaa ttacaatcca attgacagcg ccggggttat
     3541 taattaccat aaaagagtgg agcaacgaaa atttcaaaca aacgatcggt aaagggagaa
     3601 atcaaagatg agaaaagctg caattccatg gggatgctgg cgcgggagca agagcctgcc
     3661 tctgactttt ccgaataact ggaagattgc catggcgccc atggccggta agccggattt
     3721 gagccaggag gaaatccgca aggctttcca gaaccccatc ggccaggagc cgatccgcaa
     3781 gctggccgaa gggaaaaaga ctgcggcgat tgcggtggac gatttgaccc gcccgaccca
     3841 ggctcaccgc tttctccctt ttattgtgga agaactgaac caggccggca ttcaggatga
     3901 agatatcaag atcatcatgg ccgtcggctg ccaccgccca ttgatgaagg cagaccagga
     3961 gaaaaagctg ggcaagaaga tggctaaccg gtttgccgtt tacaaccacc atcctttcga
     4021 aaacgtggtc aatgtaggca ccacctcccg gggaacccca gtgattatca accgctattt
     4081 cgtggaatcc gatgtcaaaa tcgggatcgg cttcatcact ccccatccca tcgccgcttt
     4141 cgggggaggc ggcaagatcg tcatccccgg cctggggtcc atcgacacca tccaccggaa
     4201 tcatacccct gccttccagg ggaaggtcgg cagcacgggc ttttccaaag gatatgatat
     4261 caactccaat gaactccggg cggacatcga agaaggggcc cgcctggccg ggttgaatgc
     4321 catcgttaac tccgtgggga cttcggaagg caagaccgcc ggggtcttcg tgggggactt
     4381 ggtccaggcc catcgggccg ccgtcgacct ggcgaggacc gtgtaccgga cggacgctcc
     4441 ctgcgacatg gatgtcggga ttttcaacgc cttcccggaa gacacagaac tggtccaggc
     4501 ccaaaaggcc atgaatgtgt ggacgggaaa tgaaacctgc cgtttggtga gagaaggtgg
     4561 gaaagtggtc atcgccacgg ccagctccga tggtctagga ttccactccc tggcggaccg
     4621 gggcatgagg ctttacaacc gggtgggcca ccgaaaggcc atggctgata ttcttagcgg
     4681 tcgaaaggtg atcgtcttct ccccgaactg ttctcgggcc gacctcctgg aacgctaccc
     4741 agaagaagtg atcattcgga atacctggaa cgacgttctg gaagaactaa agaa
//
LOCUS       DASPRR010000378        10497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_403773, whole genome shotgun sequence.
ACCESSION   DASPRR010000378 DASPRR010000000
VERSION     DASPRR010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403773"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VEL68_14010"
     CDS             complement(<1..232)
                     /locus_tag="VEL68_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methylaspartate ammonia-lyase"
                     /protein_id="HXZ37130.1"
                     /translation="MGEIKLFGPFGNLGYGGVTEEVWEKTMAWNPDLIFQQGTSSDPG
                     PGYLGTDTSYADRAGIKHDLKRMISSAQKLGIP"
     gene            complement(558..1214)
                     /locus_tag="VEL68_14015"
     CDS             complement(558..1214)
                     /locus_tag="VEL68_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37131.1"
                     /translation="MKKMIFVGWLIVFLPALGFAQEKVEAPVWNAGDKWVFDREGPME
                     VVSCDAQCFSVRFSGGMFARDASGVAVFDRSTLNVKYMLEKDRRKAYAGFRRMIFNFP
                     LTSGKQWKDLYQVDEQGAWGGYVAVEYHETFRVLREEEVEVRAGKFKAIKMEYKLEPK
                     WRDGIGWVTGSESKAWYWYSPGVKNLVKGQYEKGYTEARMQGKGARENWELVSYELKK
                     "
     gene            complement(1834..2286)
                     /locus_tag="VEL68_14020"
     CDS             complement(1834..2286)
                     /locus_tag="VEL68_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013079930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXZ37132.1"
                     /translation="MLLSIWGWDALAQMPNPYGAPINLEAAKKIAAAAVAEARKNNWT
                     MAVAITDIAGDLVYLEKMDGTQTGSVKVALGKARSAVLFKRPTKVFEDVVASGGVGLR
                     ILLLEGAVPIEGGLPLLMDGKIVGAIGVSGGTAQQDGMAAKAGADTSK"
     gene            3014..3715
                     /locus_tag="VEL68_14025"
     CDS             3014..3715
                     /locus_tag="VEL68_14025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009706605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXZ37133.1"
                     /translation="MKVFIVHAHHEPKSFNGALTQVAQKVLSSLGDEVKISDLCAMKF
                     DPVSDRRNFTTVKNPDYLKQQAEEMHASEANGFAPDIQGEIEKVRWCDLMIWQFPLWW
                     FGVPAILKGWVDRVFAMGQAYGQGRAYEKGVFRGKQAMLSITTGGVAEAFAEKRHPLQ
                     EFREGHGFHGDIYGILRPIQRGILSFTGFAVLRPHILHAPVRLTDDERRQCLDGYAER
                     LRGIADEVPIDVGLY"
     gene            3971..4873
                     /locus_tag="VEL68_14030"
     CDS             3971..4873
                     /locus_tag="VEL68_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37134.1"
                     /translation="MPSEEEMQDVIQLTINGLLQGGIYSLISIGLTLIFGVTRIVNFA
                     HGEFLMLAMYATFFLFQLVGIDPYLSLLIVTPLLFILGLCSERLVIHPILNAPPFMQI
                     FATVGLSVALQNAALFFWTADYRSVKTAYAGMIIPAGDLLISFARLMAFMAAVLISYG
                     VFLYLKKTYAGRAIRAVAQNRTAATLMGINIRRTYFFTFGLGTALVGIAGALLMPIYY
                     VFPTVGGLFVLTAFVVVVLGGLGNMTGAFMGGLIIGLVESYSGFYVATQLKEAVYFVI
                     FILVLLLKPSGLYGIVGAEEMGEK"
     gene            4870..5883
                     /locus_tag="VEL68_14035"
     CDS             4870..5883
                     /locus_tag="VEL68_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37135.1"
                     /translation="MIRPLIRAFHEPKGYFFLACLLALVFLPLIVENPFVLHLVIMML
                     FYAALGGAWNIIGGFGGQLSLGHAAFFGLGAYTCVLLHLYTGLNPWAGMVVGMVITSI
                     FGAAISYPCFRLRGPFFTLATIAFAEVLRILATYFKNFTKGSTGITIPQNMGLANFMF
                     KEKAPYLYIILIFALLVFVVSYLIEKSRFGYYLQALREDEDAAESLGINNTWVKLKAA
                     VISAALTSIGGVFYAQYILFIEPYSEFSLDMSIQFALIPMIGGMGTAIGPIIGSFVLT
                     PLQELLRSWLGGGFAGLYLVIYGIILVLVVIFMPQGILGLFKRSESEKETLPSGGGPN
                     IRP"
     gene            5982..7247
                     /locus_tag="VEL68_14040"
     CDS             5982..7247
                     /locus_tag="VEL68_14040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37136.1"
                     /translation="MKRTKLRGWIALGLSFALLGGTSALGQETIRIGTLYPFTGPLAM
                     LGTEEWRGAETARVVQNEKGGVLGKKIEFVKADAPDAKAATSEAERLITVEKVKIITG
                     SYSSSIAYASTPVAEKYQVIYFEQGGIADNITQRGFKYLFRNNPLASDFGIRAIDLSE
                     QTVCPKIGLKPANTKVAVIYEDSLYGTTVGTSAVKYGKERGFRIVADEPYSAKAVDLS
                     SLIMKLKSAQPDILIATSYVNDAILISRQSKELKFNVKEFIGCGGGHGTKDTADALGD
                     DINYIFNVDITQPRNTSEKFCPGIAEYSSRYQKIFGHPPRSGHSLRAYNGLLIAWDII
                     KKAGSLDAEALRQAALSIDIPEGKTPTGWGVKFAPPGHPSMGTNLRAKPIVMQWFEQE
                     QYAVWPALAAVKDMVIPLPSWEERAKGKK"
     gene            7264..8010
                     /locus_tag="VEL68_14045"
     CDS             7264..8010
                     /locus_tag="VEL68_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37137.1"
                     /translation="MNSEGSPVLLRTKKVTKLFGGLAAVKEVDLEIKQGEIFGLIGPN
                     GAGKTTLFNLISGVYPPSGGTLEFRGQDYADVRHPHQVCRMGIARTFQLVKPFSNLTA
                     LENVMIGAFYRTGDPDLARKEASGIMEWMGLGDRKHMLGKSLTLPDRKRLEFARALAT
                     KPMLLLLDEVMAGLTPHEIDETLVLIRKIREEGITIFVVEHVMHAIMSLSDRIAILHH
                     GEKIMEGTPQEVARDERVIKAYLGEEYVLS"
     gene            7997..8707
                     /locus_tag="VEL68_14050"
     CDS             7997..8707
                     /locus_tag="VEL68_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37138.1"
                     /translation="MSFLSLDQVEVRYGDAIALFGISLEVLEGEVVSIVGANGAGKST
                     TIKTISGLLKPRKGQVLFQGKRLNEVAAHSIVDLGIIQIPEGRQLFPYMTTLENLELG
                     SYTHEARKKVSESLQRVFDLFPLLRERKDQLAGTLSGGEQQMLAIGRGLMALPKLLTL
                     DEPSLGLAPIMVKTIFEAISKINQAGTTILLVEQDVKKSLSLARRGYVMENGKITLQG
                     EAQELLKNEHLKRAYLGI"
     gene            8970..10184
                     /locus_tag="VEL68_14055"
     CDS             8970..10184
                     /locus_tag="VEL68_14055"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ37139.1"
                     /translation="MNPKALEGIRVLDLTRIYAGPYCSMLFADMGGEVIKIEPPEGEL
                     IRDNPPMVEQGKGGPHDRSRSGYFLTLNRNKYGITLNLKHPKALKIFKDLVKVSDIVL
                     ENYAPGVMKRLGIDYPVLKKINPRIILCSISGFGQTGPYSERMAFDVISQAMSGLMSI
                     TGHPDNPPTRVGTSLGDVNAAVHAAFAIMTALWHREKTGKGQQVDVSMLETMVAILEG
                     GIVRWTIGKELLTPIGSMNPHEAPMAAFKCKDGYIIIATVGDEHWQRFCRAINRPDWA
                     ADPALQTKAQRWAKKYFIQEEIEKVTAKYTLKEVSEMMDRERVANSPILNIKEVVEDP
                     HLKERGYFVDIEHPVIGKAKIPGIPFKMSETPGAVERPSPLVGQHNELILGKYLGIGK
                     GEIDRLKGEGAV"
BASE COUNT         2436 a         2922 c         2771 g         2368 t
ORIGIN      
        1 aaggaatccc cagcttttgg gccgaactga tcattctttt caaatcgtgt ttgatacccg
       61 cccggtcggc ataggaagtg tcggttccca gatagccggg ccccgggtcc gaagaagtgc
      121 cctgctggaa aatcaagtcg gggttccagg ccatggtctt ctcccagact tcctccgtaa
      181 cgccgccgta acccaaattg ccaaaaggcc cgaacagttt gatttccccc attctctccc
      241 tccccccatt ccttcccgga gatccttgag tcgcccgcat acaaaaaaat cgggatgggt
      301 gtgcaggaaa agcaatcctt tcccgatcat gctcagaaaa tacaaaaccg gggaatggtt
      361 gtcaagaaaa ttgaagaaca gaagggaaat agagagcagt agaagaaact cccgatcgtc
      421 tctctaacca tgcccgggag gagaggacac aaaccttttt aggcagagtt ttgaaataat
      481 cccccctttt agaaagggag aaagggggga tcaaaaagaa atttcaaagg aaggcaggaa
      541 aatggatttt caaaagtcta tttttttaat tcataggaaa ccaactccca attttccctc
      601 gctcccttcc cctgcatgcg cgcttccgta tatcccttct catactgccc tttcacaagg
      661 ttctttactc cgggtgagta ccagtaccag gccttggatt cactcccagt gacccagccg
      721 atgccgtcac gccattttgg ttcgagttta tattccatct tgatggcctt gaatttgccg
      781 gcccggactt caacctcttc ctcccttaga acccggaaag tttcatgata ttcaactgcg
      841 acatacccac cccatgctcc ctgctcatct acctgatata aatccttcca ttgctttcct
      901 gaagttaagg gaaaattaaa aatcatcctc cggaaccccg cgtatgcttt acgccgatcc
      961 ttttccagca tatatttgac attgagagtt gacctgtcga agacggcaac tccagaggca
     1021 tccctggcga acatcccccc tgaaaaccta acggaaaaac actgggcatc gcaactcaca
     1081 acctccatcg gtccttcccg atcgaaaacc cacttatccc ccgcattcca aactggtgct
     1141 tcaacctttt cttgagcaaa tcccaaggca ggaagaaaca caatcagcca tcctacgaat
     1201 atcatcttct tcattttata accctccttc ccatgaatca ggaaaataga aatggctcca
     1261 aggggacaag aaatttcctc aggaaccttt cttggggaga aaccggcgac gctaacgaca
     1321 atcactcgaa tcttgaaaat caccgaaaat tttggcttca gagggtagga gacgtaccaa
     1381 aagcgcagct atctccctgt cgcttccatc gttagatgtt tttcaggtca gatgatgatc
     1441 agctggaaaa agctgtgtcc aggcatgaat gaagaaaccc ccgagagatc agtagggcct
     1501 cccggaggct tcacatcagc attttctggc aaccccacct tcgcatttta ctcctcagaa
     1561 ggttagaggt tttgttgttg ggtttatttg gctagaggag aggaaaacga tttataaaaa
     1621 taaatcgcaa aataagttta aggaaaaaat ttcgttatct gcaaaaaata gcgtaggatt
     1681 accacgaact accttccgcg ggtgcctgtg atgaagaggg gaccggcttc aatcttttat
     1741 ccttgtccca ggacacgatt gacccttcac caagcgggtc ggcatgatca gtcgggcaag
     1801 ggaccacctg gccccttgcc ctgatgaagg cttttactta gatgtgtcgg cgccggcctt
     1861 ggcagccatt ccgtcctgct gcgcagtgcc acccgaaacg ccgatggcac cgacgatttt
     1921 gccgtccatc agcagcggca gcccgccttc aatcggaacc gcaccctcca aaagcaggat
     1981 tcgaagaccg acaccacccg atgccaccac gtcctcgaac accttggtcg gccgcttgaa
     2041 gagcactgcc gagcgcgcct tgccgagcgc caccttaacg ctcccggtct gggtaccatc
     2101 cattttctcc aagtagacca gatcaccggc gatatccgtg atggcgacag ccatggtcca
     2161 gttgttttta cgagcttcgg caacggccgc ggcggcgatc ttcttggcgg cttccaggtt
     2221 gatcggtgcg ccatagggat tcggcatctg ggcaagagca tcccatcccc aaatactcaa
     2281 caacatcaaa ctcaatacca atacgctcag actcttcttc attgactttc tcctttcttg
     2341 gctccgatta tcagagcaga attgataggg aatttctttt agaaagatgg tgcgaaagtg
     2401 tctatctgga taaatatttg acctgaagcc tttatatcgg tatcttccgg caattccttt
     2461 tcatatgaca ctccgcaagg ttataggttg ggttggaagg attcgtataa aaagggataa
     2521 gctactgctc gaatggtatc aagcctgcaa attctgtcaa gggatttttc tgagcaatga
     2581 caataataat gatggacata gaaaccggcc ggtcttattc cggcaggttt atgggcaggg
     2641 cacgcaaata agctctagcg attcgatgaa tccctttatg aagaagaatc gatcatctgg
     2701 cagaggatga ttcagctagg gaagatttcc ggttcccagc ttctttcttg acctgtatct
     2761 cagggatatt tataataccc taaacctccg acaaataggg gaagtcatgg ctcgcaggcc
     2821 ggaaagctta gcctcttttc gcccccttcg ccgcccccga gagcccgaac cttacttact
     2881 cttgagcgta ccctaggttc gatcgatccg caaaaagcaa agcttggagg gcagaatccg
     2941 aagaagcggg tcatggtctc ccgcattccc atttgcctgg ctctggctat tcagaaagga
     3001 aggtggaatt gcaatgaagg tcttcattgt tcatgctcac catgagccca agagcttcaa
     3061 cggtgcactc acccaggttg cccaaaaagt gctctcctcc ctgggggatg aggtcaaaat
     3121 atctgacctt tgcgccatga aattcgatcc cgtctcagac cgccgcaatt tcacgactgt
     3181 taaaaatcca gattacttga agcagcaagc ggaggaaatg cacgcttcgg aagcgaatgg
     3241 gtttgccccg gacatccagg gggagatcga aaaagtacgg tggtgtgatc tgatgatatg
     3301 gcaatttccc ctatggtggt tcggtgtgcc ggctattctc aaaggctggg tggatcgtgt
     3361 atttgccatg gggcaggcct acggtcaagg gcgggcatac gagaaggggg tatttcgtgg
     3421 taagcaggcc atgctttcga tcaccacggg aggggttgcc gaggccttcg cggagaaacg
     3481 tcatcccctt caggaattcc gggaaggtca tggctttcat ggcgatattt acgggatcct
     3541 ccgtccgatc caacggggaa tattgtcctt cacaggtttc gctgttctgc gtccccatat
     3601 ccttcatgcg cctgtccgtc tgaccgatga cgaacgacgg cagtgcttgg acggctacgc
     3661 cgagcgtctc cgcggtattg cggatgaagt tcccattgat gttgggttgt attaagggga
     3721 agcagcgaga tgtaaatccc tcaccccaaa agccgtgcct ttcctccttt tgacctctgg
     3781 aaggctggta ttcggtcact ccctcaaaag cgggattcca gaaaagcccc aaaaataccg
     3841 gtattctgcc ttcaaaggaa agacaaaatt atgtcccttg agcctctttc aaaggcctct
     3901 cctttttccc ttgacaaagg aaacaggcct cggtttaaat gatttgggtt caattccgca
     3961 agccgagaaa atgccgagcg aggaagagat gcaggacgtc atccagctga ccatcaatgg
     4021 ccttctccag gggggcatct attccctgat cagcatcggt ctgacgctga tcttcggggt
     4081 cacacggatc gtcaacttcg cccacggcga attcttgatg ctggccatgt atgcgacctt
     4141 tttcctattc caactggtgg gcattgaccc ttatctttcc cttttgatcg ttaccccgct
     4201 tctttttatc ctggggcttt gttcggaacg gctggtcatt catcccatcc tgaacgctcc
     4261 tcccttcatg cagatcttcg ccacggtggg actttccgtg gctctccaga atgccgccct
     4321 tttcttctgg accgcggact accggtcggt caagaccgcc tacgcgggaa tgatcatccc
     4381 cgcgggagac ctcttgatca gctttgcgcg gctcatggct tttatggccg ccgtgctgat
     4441 cagctacggg gtctttctct acctgaaaaa aacgtatgcc ggtagggcca ttcgcgccgt
     4501 ggcccagaat cgcaccgccg ccacgctcat ggggatcaac atccgtcgga cctacttctt
     4561 tactttcggc ttgggcaccg ccctcgtggg aattgcggga gcccttctga tgcccatcta
     4621 ttacgtgttc cccaccgtgg gaggactctt tgtcctcaca gcttttgtgg tcgtcgttct
     4681 gggcggactg ggcaatatga ctggtgcctt tatggggggg ctcatcatcg ggctggtgga
     4741 atcctacagc ggtttttatg tggccaccca gctaaaagaa gcggtctatt ttgtcatctt
     4801 tatcctggtc ctgctgctca agccttccgg gctctatggc atcgtggggg cggaggagat
     4861 gggggaaaaa tgatccgacc cctgatccgg gcattccacg aaccgaaagg ctacttcttc
     4921 ctggcctgtt tgctggccct ggtctttttg cccctgatcg tggagaaccc ctttgttctc
     4981 cacctggtca tcatgatgct cttttacgcg gccttgggag gcgcctggaa catcatcgga
     5041 ggctttgggg ggcagctctc gctcgggcat gcggctttct tcggcctggg ggcttacacc
     5101 tgtgttctcc tgcacctcta taccggcctg aatccctggg ccgggatggt cgtcggaatg
     5161 gtcataacct cgatcttcgg ggcggccatc agctatccct gctttcgtct ccggggcccg
     5221 ttcttcaccc tggcgacgat tgcctttgcc gaagttctgc gcatcctggc cacgtacttc
     5281 aagaatttca ccaaggggtc gacgggaatc accatccccc aaaatatggg cctggccaac
     5341 ttcatgttta aagagaaggc gccctacctt tatatcattc tcatcttcgc cctgctggtg
     5401 ttcgtcgttt cctacctcat cgaaaaatcc cggttcggct actacctgca ggcgctgcgg
     5461 gaggatgaag atgcggccga atccctgggg atcaacaaca cctgggtgaa actgaaggcg
     5521 gccgtcatca gcgccgccct gacctccatc ggcggggttt tttacgccca gtacatcctg
     5581 ttcattgaac cctacagcga gttttccctg gacatgtcca tccagttcgc cctcattccc
     5641 atgatcggag ggatgggtac ggccatcgga ccgatcatcg gttctttcgt gctcaccccg
     5701 ctccaggaac ttttgcgctc ctggctgggg ggcgggtttg cgggcctata cctggtcatt
     5761 tacggaatca ttctggtttt ggtcgtcatc ttcatgcccc aggggattct ggggcttttc
     5821 aagcgaagcg agagcgagaa ggagacgctg ccgtctgggg gagggcccaa catccgcccg
     5881 tgatcggcag gaaagcaggg ccacccggcc cgatcccttt ccgggtttca ccgaaaaatc
     5941 cgaaaggggc tggaacctca acaaccagga ggaatagaac catgaaaaga acaaaacttc
     6001 ggggatggat cgcgctaggg ctttccttcg cgctccttgg gggaacatca gccctcgggc
     6061 aagaaaccat ccgcataggg accctgtatc cttttaccgg gcccctggcc atgctcggca
     6121 ccgaagaatg gcgcggggcg gaaacggccc gggtggtgca gaacgaaaaa ggaggggtgc
     6181 tggggaagaa aatcgagttc gtcaaggccg acgcccctga tgccaaagcc gcgaccagcg
     6241 aggcggagcg gctaatcacc gtggaaaagg tgaagattat caccgggtcc tactcgagct
     6301 ccatcgccta tgcctccacc ccggtggcgg aaaaatacca ggtcatctat tttgagcagg
     6361 gaggaattgc cgacaacatc acccagaggg gcttcaagta tttgttccgc aacaatcccc
     6421 tggcctcgga tttcggcatc cgggccatcg atctgtccga acagacggtc tgcccgaaaa
     6481 tcggcttgaa gccggccaac acgaaggtcg cggtgattta tgaagacagc ctttacggga
     6541 ccaccgtggg aacgtcggcg gtgaaatacg ggaaggaaag gggattccgc atcgtggccg
     6601 acgagccgta cagcgccaaa gccgtggacc tctcctcgct gatcatgaag ctcaaatccg
     6661 cccagcctga catcctgatc gccacctctt acgtcaacga cgccatcctg atctcccggc
     6721 agtccaagga gctgaagttt aacgtcaagg agttcatcgg ctgcggcggg gggcatggga
     6781 ccaaggacac ggccgacgcg ctcggcgatg acatcaacta cattttcaac gtggacatca
     6841 cgcagccccg gaacaccagc gaaaagtttt gcccgggaat cgccgaatac tccagccggt
     6901 atcaaaaaat cttcggccac ccgccccgct ccggccattc tttgcgggcc tacaacggac
     6961 tcctgattgc ctgggacatc atcaaaaagg ccggctccct ggacgccgag gcgctgcgcc
     7021 aggcggctct ctccatcgat atcccggagg ggaagactcc caccgggtgg ggggtcaaat
     7081 tcgctccgcc ggggcatccc agcatgggca ccaacctgcg cgccaaaccc atcgtgatgc
     7141 agtggttcga gcaggagcag tatgcggtct ggccggcttt ggcggcggta aaggatatgg
     7201 tcatccccct gcccagctgg gaggagcggg ccaaaggaaa aaagtagaac gggacgcatg
     7261 gtcatgaatt cggaaggctc cccggtactc ctgcgaacga aaaaggtgac caaactcttt
     7321 ggcggcctgg cggccgtcaa ggaggtggac ctggagatca aacaagggga gatcttcggc
     7381 ctgatcgggc ccaacggagc aggaaagaca actctcttca acttgatctc cggggtatac
     7441 cctccgagcg gggggaccct ggagttccgc ggtcaggatt atgcggatgt ccggcatccc
     7501 caccaagtct gccggatggg gatcgctcgc acctttcagc tcgtgaagcc cttttccaac
     7561 ctgacggcgc tggagaatgt catgatcggc gccttttatc ggacggggga cccggacctg
     7621 gcccggaaag aagcttcggg catcatggag tggatgggac tgggggaccg gaagcacatg
     7681 ctaggcaaga gcctcaccct tcccgaccgc aaacgtctgg aattcgcccg ggcgctggcc
     7741 acgaaaccca tgcttctcct cctggatgag gtcatggccg ggctaacccc ccacgagatt
     7801 gatgaaaccc tcgttctgat ccgcaagatc cgggaggagg gcattacgat ctttgtagtc
     7861 gaacatgtca tgcatgccat catgtctctt tccgaccgaa tcgcgatcct tcatcacggc
     7921 gaaaaaatca tggagggaac cccgcaggag gtggccagag acgaacgagt gatcaaagcc
     7981 tacctcgggg aagaatatgt cctttcttag tctcgaccaa gtcgaggtgc gctacggaga
     8041 cgccattgcc ctcttcggaa tctccctgga ggtcctagaa ggggaggtgg tgagcatcgt
     8101 gggagccaat ggagccggga agagcaccac catcaaaacg atctccgggc tgctcaagcc
     8161 gcggaagggg caggtcctgt tccagggaaa aaggctgaac gaagtagctg ctcattcgat
     8221 cgttgacctg ggaatcatcc agatccccga agggcgccag ctgttcccct acatgacgac
     8281 cctggagaac ctggagctgg gctcttatac ccacgaggcc aggaagaagg tgtcagagtc
     8341 gctgcaacgg gtcttcgacc tttttcctct tttgcgggag cggaaggatc agctggcggg
     8401 aactctgagc ggaggggagc agcagatgct ggccatcggg cgggggctga tggcccttcc
     8461 caagcttctt acgctggacg aaccctccct ggggctggcc ccgatcatgg tcaaaacgat
     8521 cttcgaggcc atatccaaaa tcaaccaagc ggggacaacg attctcctgg tcgagcagga
     8581 tgtgaaaaag tctctctccc tggcccgtag agggtacgtg atggaaaacg gcaaaatcac
     8641 cctccaggga gaagcccagg aactcctcaa gaacgaacac cttaagaggg cctatttggg
     8701 gatttaactc atcaggtgca aaaaaatacc ataattcgag attcaccctt tcctattgtt
     8761 ccccgaagcg agcggcaaaa tgttgccgaa gtgcacctgg ggtgattttt gccccccttt
     8821 atcaaagggg ggcggggggg gatttctggt acgggcccgc ctcggaaatc ccccctttcc
     8881 cccctttttc aaagggggga ttttttgcaa tactcgattc tcgtgtcttg ctttttttct
     8941 caatttctgg gactgaggta agccgattta tgaatcccaa agcccttgaa ggaattcggg
     9001 tcctggacct gacccggatc tacgctggac catactgctc catgctcttt gccgacatgg
     9061 ggggggaggt cattaagatc gaacccccgg agggggagtt gatccgcgac aatcccccga
     9121 tggtggaaca aggcaagggc gggcctcacg accggagccg gagcgggtat ttcctgaccc
     9181 taaaccggaa taaatatggg attaccttga atttgaagca cccgaaagct ttgaagattt
     9241 tcaaagacct ggtgaaggtc tcggatattg tcttggaaaa ttatgccccg ggggtcatga
     9301 agcgcttggg cattgattat ccggttctaa aaaagatcaa cccgcggatc atcctgtgca
     9361 gcatcagtgg tttcggacag acgggacctt attcggaacg gatggccttt gatgtgatct
     9421 cccaggccat gagcggcctc atgtccatca ccggtcaccc ggacaatcct ccgacccggg
     9481 ttgggacgtc tttgggtgat gtgaacgcag cggtgcacgc cgcttttgcc atcatgaccg
     9541 ctctctggca ccgggaaaag accgggaaag gccagcaggt ggacgtctcc atgctagaga
     9601 ccatggtggc catcctggag gggggaatcg tccgatggac aatcgggaaa gaactcctca
     9661 cccccatcgg ctccatgaac cctcatgaag cgcccatggc cgccttcaag tgcaaagacg
     9721 gatatatcat catcgctacc gtgggggacg aacattggca gcgcttctgc cgggccatta
     9781 atcggcccga ttgggctgcc gacccggcac tccagaccaa ggcccagcgc tgggcgaaga
     9841 agtattttat tcaggaagag atcgagaagg tcacggcgaa atatactctc aaagaagtga
     9901 gcgagatgat ggaccgggag cgggtggcca actccccaat ccttaacatc aaagaagtag
     9961 tagaagaccc tcacctgaag gaacgcggct acttcgtgga catcgagcac ccggtcatcg
    10021 gcaaggcaaa aatccccggc attcccttta aaatgtcgga gacccccggc gccgtcgaac
    10081 gcccctctcc tctcgtggga caacacaacg agttgattct aggaaagtac ttggggatcg
    10141 gcaaagggga gatcgatagg cttaagggag agggtgcggt ttgaatctgg attgccaaat
    10201 cccgatctcc agtaacctca tttcttcccg atttcttccc gaatccctcg actcgaagag
    10261 agaagccaaa cccaagctgg gccttcctat tcaggcatac ccccccggag atggttgatg
    10321 aatcggcatc tcagagtgtc gcaataagaa agcccaatgc cagggcgaat catcccaacc
    10381 catggaagca actcctgaat tttctttagt tttttttgac taaaagatgg gcagtgaaca
    10441 taaagataaa tgatattgcg ttacttcaca ggcctttgcg cagctttttc acaactt
//
LOCUS       DASPRR010000379         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1052499, whole genome shotgun sequence.
ACCESSION   DASPRR010000379 DASPRR010000000
VERSION     DASPRR010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052499"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1621)
                     /locus_tag="VEL68_14060"
     CDS             complement(95..1621)
                     /locus_tag="VEL68_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37140.1"
                     /translation="MAGKKKSGLVWFVFLAAALLGMGSLAWAAPSGKVTLVMDNDAPT
                     MDPHRHAERSGVTVNWQMFDSLMFRQNDMKIVGGLAESYKLISPTVIQLNLRKNVQFH
                     NGEPFNAEAVKFSLERVLDPKTKSPRISSVNWLNSVEVVDNYTVRLNLKNPHPLWMED
                     LHNLAMVPPGYLKEKGDSHFAENPVGTGPYKFVKWTRGQEIQMTANTKYFKGEPRIET
                     GVIKIIPDASTRVAALLSGAVDLMRGISPEEIPVLKANPNLQVFTVPILRFQWFSLSD
                     AMNPQSPLYKKAVRQAINYAVNVPEIVNNIVGGLGTPTVVMNPMHFGYDPNVKPYPYD
                     PAKAKALLKEAGYPNGFEMTLHYTPVNMIKGDEVSQAIQAHLAQVGINMKLQKWSGVG
                     YMDIVKSGRAKPAFGINWGSFGVFDGDAILTPFFRSGQQYCFFHTPELDKIIDAQRQE
                     MDPQKRKELMSKAQQILREEAPWIFMYAFKTVEGANAKLDYKPRSDEIFSIYEIGFKK
                     "
     gene            complement(1711..2496)
                     /locus_tag="VEL68_14065"
     CDS             complement(1711..2496)
                     /locus_tag="VEL68_14065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXZ37141.1"
                     /translation="MSRDLSLVKEVLLAAALEGGKILKEYFRSEKMEPKTKSSHADLV
                     TNADIKTQEAVIQILSRDLPGVQIIGEEKENEEASGEVIYVDPLDGTLNFFHGQRQFA
                     VSLGYWVDRTPAAGVVFNPVDEDLFVAVSGQGAFRNGRPIRVSPYRRLSQSVLATGWP
                     YEKKELPQVLRSLERLLPVTQEVRIWGTAALAMCYLAAGALEGYWEWGLYPWDIAAGV
                     VIAREAGCQISSIDGGLFDLKQGAILATNGHVHEEMVRYLREE"
     gene            complement(2708..>3603)
                     /locus_tag="VEL68_14070"
     CDS             complement(2708..>3603)
                     /locus_tag="VEL68_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXZ37142.1"
                     /translation="VVLGVNNGFALKNWPEPNAWAKIIAEDLGLKEVQFSFDLLDPTL
                     PEPGRSALCQEIVKAAEKYGLSLRTTFTGLIIYAQNHLAHPNFWFRNQAWHWFESALE
                     VTSMLGAEATGGHIGAMSATDFADPSRRAFLRRTLIEAVRELTYLASRLGQEYFLWEL
                     MPTPREFPHTPEEALELIEEANAEAAVPVRICFDLGHACSYDLKKPGDPHAWLEKLLP
                     YAPVVHLQQTDGKGDHHWPFTPEYARKGIIKPKRVVEIVKKSPLRRVDLILELGHSLD
                     ASDQTILDDHKRSVEAWAKWL"
BASE COUNT          715 a         1054 c          978 g          856 t
ORIGIN      
        1 cggctttcgc cggaatgacg cccttcccct tagttgcaac acagcctccg aggggggagg
       61 aaaggatggg ggtgtttagg cgcaggttgt tggtttattt cttaaacccg atctcgtaga
      121 tggagaaaat ttcgtccgac cggggtttgt aatccagctt ggcgttggcc ccttccacgg
      181 tcttgaaggc gtacataaag atccaggggg cttcctcccg taaaatctgc tgggccttgc
      241 tcatcagttc cttcctcttc tgcgggtcca tctcctggcg ctgggcgtcg atgatcttgt
      301 ccagctccgg cgtgtgaaag aagcagtact gctgacctga acggaagaag ggggtcagga
      361 tggcgtcgcc gtcaaaaacc ccgaagctcc cccagttgat cccgaacgca ggcttagccc
      421 gtccgctctt gacgatgtcc atgtacccta cgccggacca cttctgcagt ttcatgttga
      481 tccccacctg agccaggtga gcctggatgg cctgggagac ttcgtccccc ttgatcatgt
      541 tgacgggtgt gtaatggagg gtcatctcaa acccgttggg gtacccggcc tctttcaaaa
      601 gggccttggc cttcgccggg tcgtagggat agggtttgac attgggatca tagccgaagt
      661 gcatcgggtt catgactacg gtgggggttc ccagcccgcc tacgatgttg ttcacgatct
      721 ccggaacgtt gaccgcgtag ttgatggctt ggcgcaccgc tttcttgtaa agcggagact
      781 ggggattcat cgcatcggag agagagaacc actggaaccg caggatggga acggtgaaca
      841 cctgcaggtt cggattggct ttgaggaccg ggatttcttc cggcgagatc cccctcatga
      901 ggtccaccgc tccagacagg agggcggcca cccgggtcga tgcatcggga ataatcttga
      961 tgactcccgt ttcgatccgc ggctccccct tgaagtattt ggtgttggcg gtcatctgga
     1021 tttcctgccc ccgggtccat ttcacgaatt tgtagggtcc cgttcccact gggttctcgg
     1081 cgaaatggga atcgcccttt tccttcaggt atcccggggg aaccatggcc aggttgtgaa
     1141 ggtcttccat ccacaaagga tggggattct tcaggttcag cctcaccgta taattgtcca
     1201 ccacctccac cgagttgagc cagttcacgg aagagattcg cggggacttg gtcttggggt
     1261 cgaggacccg ctccaggctg aatttgacgg cctccgcatt gaaaggctcg ccgttgtgga
     1321 attgaacgtt tttcctcaaa ttcagctgaa tcaccgtggg gctgatgagt ttatagcttt
     1381 cggccagccc ccccacgatt ttcatgtcgt tctggcggaa catgagggaa tcgaacatct
     1441 gccagttcac cgtgaccccc gacctctctg catggcggtg cgggtccatg gtcggcgcgt
     1501 cattatccat gaccagggtg accttgcccg agggagccgc ccaggctaaa gaccccattc
     1561 ccaaaagggc ggcggccagg aaaacaaacc aaaccagtcc agatttcttc tttcctgcca
     1621 tttctttctc tcctttctgt ttcatcattc cgcagggacg gcgtgatcgg ggatcacgcc
     1681 ctacagaaca tttttccatt ccgcatttaa tcattcttcc ctcaaatatc ttaccatctc
     1741 ctcgtgcaca tgcccgttgg tcgccaggat cgctccttgc ttcaggtcaa aaagtccccc
     1801 atcgatggag gagatctggc atcccgcttc ccgggcaatc accactcccg cagcaatatc
     1861 ccagggatag agcccccatt cccagtaccc ttccaatgct cccgcagcca gataacacat
     1921 ggccagtgcc gcagttcccc aaatccttac ttcctgcgta accggcagca ggcgctccag
     1981 ggatcttagg acttggggga gctccttttt ttcgtagggc cagcccgtgg cgaggaccga
     2041 ctggctgagg cggcggtagg gggaaacccg gatcggcctg ccgttccgga acgccccctg
     2101 cccggaaacg gctacgaata aatcttcatc taccgggttg aacaccacac cggcagccgg
     2161 cgttcgatcc acccaataac cgagggagac ggcaaattgc ctctgcccgt gaaagaaatt
     2221 cagggttccg tccagggggt ccacatagat cacctccccc gaggcttctt cgttctcttt
     2281 ctcctccccg atgatctgca cccccgggag gtcccgggac aggatttgga tgacggcttc
     2341 ctgagtctta atgtcggcgt tggttaccag gtcggcatgg ctggacttgg ttttcggttc
     2401 catcttctcc gagcgaaaat actctttgag aattttcccg ccttctaggg cagcggccag
     2461 gaggacctct ttcaccaggc ttaaatctct ggacaaaaac ctctccctct tttgccattc
     2521 aaatatgatg atctcataaa aagtcgaatt tccctaaaat ttgtcattcc ggcgaaggcc
     2581 ggaatctagt tatttcaagg cgttctggac cccggctttc gccggggtga caattcaaga
     2641 gactttttac gacaccacca aatatggagt ttctttctgc tccgtgcatt ccgaatcccg
     2701 caatatatca aagccacttg gcccaggctt ccacacttcg cttgtgatcg tcaagaatgg
     2761 tttgatcgct agcatccagg gaatggccca gttccaggat cagatcgact cgtctcagcg
     2821 gagacttttt tacgatctcc acgactctct tgggcttgat gattcccttc ctcgcatact
     2881 ccggtgtgaa gggccagtgg tggtctccct tcccgtctgt ttgctgcagg tggacgacgg
     2941 gagcgtaggg tagaagcttt tccagccaag catggggatc tccgggcttt ttcaggtcgt
     3001 aggaacatgc gtgtcccaaa tcgaaacaga ttcgaaccgg cacggcagcc tccgcattag
     3061 cctcctcaat cagttccagg gcctcctccg gagtgtgggg aaattctcgc ggggtgggca
     3121 tcagctccca caggaaatat tcctgcccca gccgggaagc caggtaggtc agctcccgga
     3181 ccgcttcgat cagcgttctc cgcagaaagg ctcgccgtga tggatcggca aaatcggtgg
     3241 cgctcatggc cccgatgtgc cctcccgtgg cctccgcgcc gagcatgctg gtcacttcca
     3301 gggccgattc aaaccaatgc caggcctgat tccggaacca gaagttgggg tgggccaggt
     3361 gattctgagc gtaaatgatg agaccggtga aagtggtgcg gagggagagg ccgtattttt
     3421 ccgcggcctt cacgatttcc tggcacaggg cgctcctccc gggctcgggc agcgtggggt
     3481 ccagaaggtc gaaggagaat tgaacctcct tcaagcccaa atcctccgcg atgattttcg
     3541 cccaggcatt cggctccggc caatttttca gcgcaaaccc gttattcact cccagcacga
     3601 ctt
//
LOCUS       DASPRR010000380         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_91724, whole genome shotgun sequence.
ACCESSION   DASPRR010000380 DASPRR010000000
VERSION     DASPRR010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91724"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VEL68_14075"
     CDS             complement(<1..333)
                     /locus_tag="VEL68_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXZ37143.1"
                     /translation="MSPQRTQVPLTKKLEALSKISKAISSDLYLEDILRLIVVVTAEV
                     MKSKVCSLWLLDERDSALKIRATQAMSEEYLKERSLKLGEGVVGYVFQENRPMMIYNV
                     LKEPRYKEK"
     gene            651..1577
                     /locus_tag="VEL68_14080"
     CDS             651..1577
                     /locus_tag="VEL68_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ37144.1"
                     /translation="MGKDKTGMTRRDFLKTASAGLAMGMAGRPDWLLAKEDYDLAVIS
                     GDPAVATKKAIETLGGMSRFVKKGQRIVLKPNMSFANPPDRGSTTHPAVVVTVAQACL
                     EAGAQRVMVLDYPLRKGELCLERSGMLDAFQSLKGVHVLALEERKFYREIKVPQGKIF
                     DRVEIMREVLESDVLINIPQAKSHSATGVSLGLKGLMGVIWDRYSFHSKFDIDQAIVD
                     LATVIKPQLTILDATRALVSGGPGGPGEVVKPNLIIAGTDPVAVDSYAVSITPWYGQN
                     FKGRQVKHILGAHQRGLGRIEFESLRILKEKA"
     gene            1574..>2667
                     /locus_tag="VEL68_14085"
     CDS             1574..>2667
                     /locus_tag="VEL68_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ37145.1"
                     /translation="MRTLIQALSLTLFSLLFFFFANYKLPDWLPADIYLRLDPLLGLG
                     AILSAREWIGRVLWSLILIGATLAAGRFFCGYVCPMGVAIDFLDPLLGRRKGRPDLKK
                     EVPWRKVKFFALVLFLSSSLAGLTLAYLMDPLPLLTRFYTFFLYPLVITLINLVLDVL
                     RPLTQALGWVGLSYQHYTQPVFYMALITFVVFSGIIALNRITPRFWCRYLCPLGAFLS
                     LISPLGFFKRRVSQECNECLKCVRSCPMGAIEEDPKKTRLPECIQCRQCSKVCPKEAV
                     SFPALRSTIQTRQLGGEYSRVDLSRRGFLYSFTGGLTAAFLADRTPFTSVQGKHQLVR
                     PPGALPEEEFLRTCIRCGECMKSCVTNTLQP"
BASE COUNT          523 a          771 c          726 g          647 t
ORIGIN      
        1 cttttctttg taccggggtt ccttcaggac gttgtagatc atcatgggcc tgttttcctg
       61 aaaaacgtat cccaccacgc cctctcccag tttaaggctt ctctctttca ggtattcctc
      121 gctcatcgcc tgagtggccc ggattttcag ggcgctgtcc ctctcgtcca agagccagag
      181 ggagcaaacc ttggacttca tcacctccgc ggtgaccacc acaatgaggc gcaggatgtc
      241 ctccaggtag aggtcggagg agatagcctt gctgatcttg gagagggctt ccagcttttt
      301 ggtcagcgga acttgggttc tctgaggtga cattctcttt tccattaacc ggcgtttgct
      361 agcatttgat attataatcc acgccctttg tcaaggactc ttcgtcccaa gagtgacggg
      421 tcgtcctttt tggccacagc agccattccg ccccgagatt cctcttttga atccatcccc
      481 catgggttat gattttatct agcttgacca cggggatgag agggaaacct tttattccca
      541 tctatcgtct cctgctgacc caaatcatag gacagaaggg gatcaaaacg gctaaaatat
      601 aagttgatct gtggcagaaa tttgattcgc cgaggaggaa agaaaaggga atggggaaag
      661 acaaaaccgg aatgacccgc agggactttc tcaagacagc gagcgccggc ctggcgatgg
      721 gaatggctgg caggccggat tggctgctgg ccaaagagga ttatgacttg gcggtcattt
      781 ccggcgaccc ggcggtggcc acgaaaaagg cgattgaaac tctgggggga atgtcccgct
      841 tcgtgaaaaa ggggcagcgg atcgttctga aacctaatat gtcttttgcc aatcctccgg
      901 accggggttc caccactcat cctgcggtag tggtcacggt ggcccaagcc tgcctggagg
      961 ccggagccca gcgggtgatg gtgctcgatt acccgctgcg caaaggggaa ctctgcctgg
     1021 agcggtccgg gatgttggac gccttccagt ccctcaaagg tgttcatgtc ctggctctgg
     1081 aggagaggaa attctaccgg gaaatcaagg tcccccaggg aaaaatattc gaccgagtgg
     1141 agatcatgag ggaagtactg gaaagcgatg ttctcatcaa cattccccag gccaaatccc
     1201 attccgcgac aggggtcagc ttgggcctca aagggctcat gggagtgatc tgggaccggt
     1261 acagtttcca ttccaagttt gacatcgacc aggcgattgt cgacctggcc acggtgatca
     1321 aaccccaatt gaccatcctg gatgcgaccc gggctctggt ttccggtggc cctggcggcc
     1381 cgggcgaggt agtcaagccc aacctgatca ttgccgggac cgatccggtg gcggtggact
     1441 cctacgcagt cagcataacc ccctggtatg gacagaactt caagggccgt caggtcaaac
     1501 atattctggg cgctcaccag cggggactgg gaaggattga gtttgaatct ctcaggatcc
     1561 tgaaggagaa agcatgagga ctttgatcca ggccctctcc ctgactcttt tctccctcct
     1621 attctttttc tttgctaatt acaaacttcc cgattggctg cccgcggata tctatctgcg
     1681 cctggatccc cttttggggc tgggcgccat tctttcggcc agggaatgga tcggccgtgt
     1741 cttgtggtct cttatcctaa taggagccac tctcgccgcg ggccgcttct tctgcggcta
     1801 tgtctgcccc atgggggtgg ccatcgattt cttggatcct ctcctgggac gcagaaaagg
     1861 ccgcccggac ttgaagaagg aggtcccctg gaggaaggtg aagttctttg ccctcgttct
     1921 tttcttgagc tcttccctgg cgggcttgac cctcgcctac ctcatggatc cccttcccct
     1981 gctgacccgt ttctacactt tttttctcta ccccctggtg atcaccctga tcaacctcgt
     2041 tctggacgtc ctgcgccccc tgacccaggc cttgggatgg gtgggccttt cctatcagca
     2101 ctatactcag ccggtctttt acatggcgct catcaccttt gtagttttct ctggaatcat
     2161 cgccttaaac cgaataacac cccgcttctg gtgccggtat ctctgtccgc tgggagcttt
     2221 cctgagcctt atctctccct tgggcttctt caagcggcgg gtaagccagg aatgcaacga
     2281 gtgtctcaag tgtgtacgct cttgccccat gggagccatt gaggaggacc cgaagaagac
     2341 gcgtctgcct gagtgcatcc agtgccgcca atgttccaag gtctgcccga aggaggcagt
     2401 ctcctttccg gccctccgct ccacgatcca gacgcgtcag ctcgggggag aatattcccg
     2461 ggtggacctt tcccggcggg ggtttctgta ttccttcacc ggagggctca ccgcggcttt
     2521 ccttgccgac cgaaccccct ttacctcagt ccagggaaag caccagctgg tgcggccccc
     2581 cggagccttg cccgaagaag aattcctgcg cacgtgcatc cgctgcgggg aatgcatgaa
     2641 gtcctgcgtc actaacactc tccagcc
//
LOCUS       DASPRR010000381         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2182703, whole genome shotgun sequence.
ACCESSION   DASPRR010000381 DASPRR010000000
VERSION     DASPRR010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182703"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VEL68_14090"
     CDS             <1..763
                     /locus_tag="VEL68_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXZ37146.1"
                     /translation="EVDGRFPNHHPDPTIPEYMQALIAKVKETGAEVGIGYDGDADRI
                     GAVDHLGNIIWGDQLMILFSRQVLRNHPGATIVSEVKCSQNLYDDITRNGGRGIMGRA
                     GHSLLKKQMKEEKALLGGEMSGHIFFADRYFGYDDAIYASCRLLEIISQAERRIPDLL
                     SDVPKTYSTPEIRVDCPDDKKFSIAEKVKESFRKEYPVVDIDGVRVLFPDGWGLVRAS
                     NTQPVLVLRFEARTLKRLEEICALVEKKVVELSKE"
     gene            936..2042
                     /locus_tag="VEL68_14095"
     CDS             936..2042
                     /locus_tag="VEL68_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HXZ37147.1"
                     /translation="MNKPDLYAVIMAGGGGTRFWPWSRQMRPKQVLPILSARTMIRET
                     VDRVQPLVPRERILIVTSRSQARQLHREVDRIPPENLLLEPEGKNTAPCLGLAAVHVQ
                     RKNPEAVLIVLPADHYIGAPEKFLRTLRRAAEFASRQNYLITLGITPTGPETGYGYIQ
                     KGPVLGRVKETEVFRAKAFREKPALQKAQDYLRRGDFLWNSGMFIWRVGVFLKALEKY
                     LPRLYGDMQELKSSIGKRGERRVLEKVYSQCPSISVDYGVLEKAPNVALIEARFGWND
                     VGSWAVLWNLWPKDPAGNVCIQGGERGESKVLVLDSSGCLVRGEKSFIAVLGLKDTLV
                     VEAGDAILVCPRSRSQDVRRVLQELKERGWQKYL"
     gene            2226..>2435
                     /locus_tag="VEL68_14100"
     CDS             2226..>2435
                     /locus_tag="VEL68_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009628776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXZ37148.1"
                     /translation="MLIIPAIDIRGGKCVRLFQGDYRKETVYGEDPGAMAEKWVSQGA
                     RFLHLIDLDGAREGVPQNRITIQAIA"
BASE COUNT          571 a          673 c          699 g          492 t
ORIGIN      
        1 cgaggtcgac ggccgttttc ccaaccatca tcccgacccc accatcccgg aatacatgca
       61 agccctgatc gccaaagtga aagaaaccgg ggcggaagtc ggaatcgggt acgacggcga
      121 cgccgatcgg atcggcgccg tggaccattt aggaaacatc atctggggag accagctgat
      181 gattctcttc tcccggcagg tcctgaggaa ccatcccgga gctacgattg tctccgaagt
      241 gaaatgctcc caaaacctgt atgacgacat taccaggaat ggcggtcggg gcatcatggg
      301 acgagccggt cactccctcc tcaagaagca gatgaaggag gaaaaggcgc ttcttggggg
      361 agagatgagc ggacacatct ttttcgcgga ccgttatttc ggttacgacg atgccatcta
      421 cgcttcctgc cggctcctcg agattatttc ccaggccgaa aggcggattc ccgaccttct
      481 cagcgatgtc ccgaaaacct acagcacccc cgagatccgg gtggattgtc cggatgacaa
      541 aaaattctcg atcgctgaaa aggtcaaaga atctttccgt aaggaatacc ccgtcgtgga
      601 tatagatggg gtgcgggtcc tctttccgga cggctggggg ctggtccggg cttccaacac
      661 ccagcctgtc ctggtgctgc gcttcgaagc gcgaaccctg aagcggctgg aggagatctg
      721 cgcgctggtg gagaagaaag tggtagaact ctcaaaagaa taaaaagcca tagagcaaag
      781 cgcataccca atagcccgaa aagcagaacc atttgatagc ccgacatccg aatcccgcca
      841 cacggttcgc aggcaataat tctggggagt ctttcgttct aacacgacga tctatccgcc
      901 atgtccgttt ggatttcgga ctggaataag ctcatatgaa taaacccgac ctatatgcgg
      961 tcatcatggc cggcggcggc gggacccgtt tctggccctg gagccggcag atgcggccca
     1021 agcaggtcct tcccatcctt tctgccagga ccatgatccg ggaaacggta gaccgggtcc
     1081 agcccctggt tccccgggaa cggattctta tcgttacctc ccgttcccag gcccggcagc
     1141 ttcaccgcga agtggaccgc attcccccag aaaaccttct cctcgagccg gagggaaaga
     1201 acacagcccc ttgtctcggc ctggcggcgg tgcacgtcca gaggaagaat cccgaagcgg
     1261 tacttattgt tctgccggcg gatcactaca tcggagcccc cgaaaaattc ctgcgtaccc
     1321 tgcgaagggc ggccgaattt gcctcccgcc aaaactatct catcactctg ggaatcaccc
     1381 cgaccgggcc ggagacggga tacggatata tccagaaagg gccggtcctc ggtcgggtca
     1441 aggaaacgga ggtgttcaga gccaaggcct ttcgggaaaa gccggccctg cagaaagccc
     1501 aggactacct gcgtcgggga gatttcctct ggaacagcgg catgttcatc tggagggtag
     1561 gggtattctt aaaggccctg gaaaaatatc tcccccggct ttacggggat atgcaggagc
     1621 tgaaatcctc cattggaaaa aggggggagc ggagggtcct ggagaaggtg tattcccagt
     1681 gcccttccat ttccgtcgac tatggggttc tggagaaagc accgaatgtg gccttgatcg
     1741 aagcccggtt cggctggaac gatgtgggaa gctgggctgt tctgtggaac ctctggccca
     1801 aagaccccgc aggaaacgtt tgtatccagg ggggggagag gggggagagt aaggtcctcg
     1861 tcctcgatag ctcgggctgt ttagtccgtg gcgaaaagag cttcattgcg gttctcggac
     1921 tcaaagatac cctagtggtg gaagcgggcg atgcaatcct ggtctgtccc cgaagccggt
     1981 cccaggacgt ccgccgcgtc ctccaggagc tcaaggagag aggctggcag aaatacctat
     2041 aactccagat gggtctagtg caaagcccat aatacatagc gtgaaagacg aaaattcaag
     2101 tagaagatcg gttttttcct gctgtgctct ccgtgctggg cagcttgcaa agcttggatt
     2161 tcgaattttt tgactttcca aattgaggcg cttaagcaca tttaagtata cttaaggcac
     2221 ttttcatgtt aatcatcccg gccatcgaca ttcgcggagg aaaatgcgtc cgcctcttcc
     2281 agggcgacta tcgaaaagag accgtgtacg gggaagaccc cggggccatg gccgagaaat
     2341 gggtttctca gggcgcccgg ttccttcatc tgatcgacct ggacggcgcc cgggaaggag
     2401 ttccccaaaa ccgaattacg atccaagcca tcgcc
//
LOCUS       DASPRR010000382        13986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_91958, whole genome shotgun sequence.
ACCESSION   DASPRR010000382 DASPRR010000000
VERSION     DASPRR010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13986)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13986
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91958"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VEL68_14105"
     CDS             complement(<1..105)
                     /locus_tag="VEL68_14105"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ37149.1"
                     /translation="MRHPMFSEEHELFRDAVRKFVEKELTPHAEEWEEA"
     gene            complement(294..1832)
                     /locus_tag="VEL68_14110"
     CDS             complement(294..1832)
                     /locus_tag="VEL68_14110"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXZ37150.1"
                     /translation="MEMTLGYSVVRNSRKHPHKTAIRCGDVRRTYRELNDRSNRLARA
                     LLNLGLRKGDRIATLSFNSIELMESYLAHLKIGAITVPLNAWGMDKDILYQAVFTGCK
                     LLVYTQDFLPRVEKLRPSLPDVQEWVFIGDPLPVFARPYEKLMAGESPDDFPIEVKEE
                     DEAFILFTGGTTGTPKGAVLTHKSLLWNIISVTTENQSPTPDDVVYYPMPLFHTAALS
                     RFLAYMYAGGTFIVTKEFNARKCLEIIEEEKVTAMTGNPTIWGRLLQEMESRAYDTAS
                     MRMYLSSQGFLHPTMQQAIETRLFPRAQTYVSYALTEASPGVTILKPTDRPKETGSVG
                     KPYMCTEVRIVDDQDRDRPVGEVGEIIIKGPTVMKEYYHNPEETAQTLRGGWLHTGDL
                     GKYDDDGFLYFVDRKKDMIKTGGLNVYSKEIEEVLSRHPKVAEAVIIGIPDEKWGETI
                     KAVVVPKGGMDLTESEVIDFCKAHLASYKKPTSVAFIEALPKTQFGGKVLKRELRERF
                     SKKE"
     gene            complement(1995..3437)
                     /locus_tag="VEL68_14115"
     CDS             complement(1995..3437)
                     /locus_tag="VEL68_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HXZ37151.1"
                     /translation="MKTPEQYIESLRKLNLEVYLLGERVKNPVDHPIIRPSLNSVTMT
                     FQLAQQDEHKAWMTTTSHLTGKTINRFTAIHQSSQDLVNKVKMQRLLGQKTASCFQRC
                     VGMDAMNALYSTTFEADEKHKTSYHRRFKEFLQRVQEEDLVVDGAMTDVKGDRSLRPV
                     QQADPDMFLRVVERRKDGVVVRGAKAHQTGMINSQEVIVMPTMALREDEKDYAISFAV
                     PTDAKGLFFIYGRQSCDTRKMEGSSLDVGNHQFGGHEVLAIFDDVLVPWDRVFLCGET
                     DFVGALVERFAGYHRQSYGGCKVGVGDVLIGAASLAAEYNGAAKASHIGDKIIEMVHL
                     NETLYSCGLACSSEGHATRAGNFEIDMLLANVCKQNVTRFPYEIARLAEDIAGGLMVT
                     MPSEKDLRSERTGPFVEKYLKGVASVPTEHRMRVLRLIENLTLGTAAVGYRTESMHGA
                     GPPQAQRIMIRRQADLEVKKNLAKKIAGIG"
     gene            complement(3534..4673)
                     /locus_tag="VEL68_14120"
     CDS             complement(3534..4673)
                     /locus_tag="VEL68_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ37152.1"
                     /translation="MIDFALSEQQKAFQKTGRDFAAKEIAPAALDYDRNPRFPGDIIQ
                     KAHAAGLMNLTCPKQYGGQGLGLVDSSIINEEMNAACVAISGMIGINSLACGPILAGG
                     SDELKRRFLLPLNQSGKTASFGLTEREAGSDAGGLKTRAKLEGDHYVLNGQKCFITNA
                     SYAALYSIFATIDPSKGTKGICGFVVPRESPGLSIGKVEDKMGQRSLNVAEVILENVV
                     VPRENRLGNEGEGFKWAMEALDEGRVNIATVGLGLSRAAFEAALSYAKTRVQFGRPIG
                     THQGLNFMLADMAAAVEASRLLTWYAGSLADQHKRFTREAAQAKFFATDTAMRVTTDA
                     VQVHGGYGYTKDFIVEKLMRDAKLTQIYEGTNQINRMVAGGALMR"
     gene            complement(4800..5249)
                     /gene="dtd"
                     /locus_tag="VEL68_14125"
     CDS             complement(4800..5249)
                     /gene="dtd"
                     /locus_tag="VEL68_14125"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXZ37153.1"
                     /translation="MRAVIQRVSRAQVRVNGQSTGSIGPGLLVLLGVGRGDTSQEADY
                     LLDKIVHLRIFEDAQGKMNLSLLEVGGELLVISQFTLYADCRKGRRPSFTDASAPDDA
                     QKLYDYFVGAARTRGLKVATGIFQAIMEVELVNFGPVTIFLDSSKNF"
     gene            complement(5380..6723)
                     /locus_tag="VEL68_14130"
     CDS             complement(5380..6723)
                     /locus_tag="VEL68_14130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXZ37154.1"
                     /translation="MIDKKNDEILSPRRKRLWFIWALSLAGAFLVAAGFFFFSGLERN
                     PSTEAGVGLPQTASPPPPLPDRVISGQIFKGQNLSSALRAQDLPKDLVEIICRHLKEV
                     INLRRVKPGDSFEVRLSPQGQFLDLTYTVGPLDIYQLSLTSAGEWIASKKEVPVNAYW
                     ARVSGEITSSLFEAMDRLGEKDSLIMDFADIFAWEIDFHVDPQPGDRFCMVVEKYFTG
                     DVFVRYGRILYAEYQREARNHQAVYFQEPGKRGDYFTPQGESLRKAFLRSPLKFTRIS
                     SGYSQSRRHPILGEYRPHLGIDYAAPQGTPVWAVADGTVISCGWNGGYGKQVVVRHPK
                     GYQSMYGHLSGYGPGISKGKPVHQKQIIGYLGSTGLSTGPHLDFRLLRNGVPRNPLRE
                     VSPRAPALTNEQMPEFQKAMNPLLRWMDDPSASPRQMVASLTSLDLEGSIKEKGK"
     gene            complement(6806..8047)
                     /locus_tag="VEL68_14135"
     CDS             complement(6806..8047)
                     /locus_tag="VEL68_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXZ37155.1"
                     /translation="MARKGFWKTLAVFFALFGPGIITSNVDNDAGGITTYSLAGSEYG
                     LSLLWTLFPITLALIVIQEMCARMGVVSGKGLSDLIRERFGARATFYLMIALFLTNLG
                     NTISEFAGVAASLEIFGVSKYLSVPLSAFFVWWLVVKGSYKSVEKAFLVACLFYISYI
                     VSGFMVKPDWGEITAHFLTPTLGTMELTPEYLTMAVGVVGTTIAPWMQFYLQSSVVDK
                     GLKAEDYQYARMDVVFGSIIVNIVAFFIIMLCALTIFQAGIKIETAKDAALALAPLAG
                     KYCTWLFAFGLLNASLFAASILPLSTAYTICEAFGWEASVNRKFAEAPQFYGLYSLMI
                     ILGAAVILFPKIPLIPIMFYSQVINGVLLPVILIFMLILVNDRRIMGKFTNGWVLNLI
                     SFVTIAVLILLSGAMVVTTFR"
     gene            complement(8142..9404)
                     /locus_tag="VEL68_14140"
     CDS             complement(8142..9404)
                     /locus_tag="VEL68_14140"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXZ37156.1"
                     /translation="MASSPNSQVFLFLSQVLGKSIVDSAGQFQGKVLDLVAVVADLYP
                     PVTALLYLPRGAQSPARLPWNKVTDIFGPVVTSSPVSPDVSSGNRNEGELLLKESLLD
                     KQIVDTHGAKILRVNDLHLLKVNQNLFLVHVDVGFRGLMRRVGLEKATDKFFQWFFDY
                     HLADHFIPWRFVQPLSSPDLLRLKIAQNRLSLLHPADLADIIEDLDIHKRTEVFQSLD
                     VETAAEALEETDPKIQVRLIENLNPAKASDIIEEMSPSEAADLLGDLPKEKAEGILKE
                     MEKEIAEDVKGLLEHPEEKAGGLMTTAFATLPPRATAREALELLRQEADNLDVIDYAY
                     VADPEEHLLGVISLRDLLIASPQTRAEDMMDSRVVNVGLDDEKEEIAKLFAKYGLKAI
                     PVVDEENHIKGVIPFKSLLEVVAPELGK"
     gene            9570..10943
                     /locus_tag="VEL68_14145"
     CDS             9570..10943
                     /locus_tag="VEL68_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HXZ37157.1"
                     /translation="MIKPIPLKPFFSRHLSPERIFISGFAGYILLGALFLWLPLSAAK
                     NPVPFIDALFTSTSAVCVTGLASIDIGTSFSFFGQVVTILLFQVGGLGIITFSTIFFV
                     LMGRGISFKGREIVQSTFLHTPRADFFAILKSVLWFTLVFETIGILFLWYRFCYDFPV
                     GHAFYQAVYHAISAFNNCGFSLFPDNLVRYQGDFIVNLTVMTLIVVGGIGFVVQHEVF
                     AKLRGRQRKLSVHTRIVLLATGILILGGAILIYIFERNHILYGMPGQNQILAIFFQSV
                     VPRTAGFNTVDIGFLTNATILLMIILMFIGASPGSTGGGVKTTSASILFLLMWARLRG
                     NEEVNVFKRTIPREVVSRTIGIIFASGFSVSLVTSALLFFSSGDLGPLESRHYFVEYL
                     FETVSAFGTVGLSMGVTPDLNVAQKLAIILMMYAGRVGPLTLAFSFSRKALAKSLTYA
                     EEGVMVG"
     gene            10956..11603
                     /locus_tag="VEL68_14150"
     CDS             10956..11603
                     /locus_tag="VEL68_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HXZ37158.1"
                     /translation="MKRVVVVGLGIFGFNIAKDLFENGFEVIAIDKNKDVVNRIRDFS
                     TKAVLADATDRELLEMIGIQPDDLVIISFGEDLAASTLLTLHLKEMNIKSILVKAPNE
                     DHKRILEKVGATEVIIPEKAMASKVAKGLISPNVLDFLPISEDFTISEVAPPAGFLGK
                     TLADLRLRGKYHVEVIAIRDVLSDKIQLVPRADFVIKDSDILIVIGKEADIQKIQ"
     gene            11659..12096
                     /locus_tag="VEL68_14155"
     CDS             11659..12096
                     /locus_tag="VEL68_14155"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HXZ37159.1"
                     /translation="MQPLMAQEKITLGLVEEIILLPWMVKIPARIDTGAAVSSLDVRG
                     LTVKRKTAEFNLSPKYSGARISVPVVGWHWVRSAESREKRPIVEIDLCVGPKKLRARV
                     NLNDRSQVQYPFLIGRNILKENFVVDCMKEYCNPPSCPDLPQK"
     gene            12093..13622
                     /locus_tag="VEL68_14160"
     CDS             12093..13622
                     /locus_tag="VEL68_14160"
                     /inference="COORDINATES: protein motif:HMM:NF025760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UUP1 family membrane protein"
                     /protein_id="HXZ37160.1"
                     /translation="MKRPALWLALSLFLLTAALLFYRIVWLGYPVFPTAPGRTWELTI
                     NARVMSDTGEARVLLGLPTENPGRMLLEERISSGSLSLSLAREGPNRIGIWSGPVELE
                     SQEITYRATILIRPRQRPPEHPSGIGPYPDRFDREETALAERLAGRWKGLPPSQLVRA
                     IAATAKGEWKNPPPEMKDLDGWKVLSRTYGREESLLLLLRAANLPARLVEGLPLVESI
                     QNQLLTWVESWNGNHWEIILPERGEVVSNPAGYLPLSVGGVPALRVSIGARADIRWSL
                     SRQVVSQWSLHWERIRRSNRFLDQWSLFRLPPEFQRTFRVLLLVPIGALIIGFLRNMI
                     GFPTFGIFMPMLMALAFRSTGLAYGLSIFGGVLLVGYAVRRGLDKLRLLLVPRMSVIL
                     TLVIACFTFIALVGNKFGMREFMAVGLLPFVILTMVIERFFVLVEEAGIREGVKTAAG
                     SAAVSIIAYGIISWEPLQLTFFVYPELLAAVAAVQILLGRYTGYRLSELFRFRGVGGS
                     S"
     gene            13619..>13986
                     /locus_tag="VEL68_14165"
     CDS             13619..>13986
                     /locus_tag="VEL68_14165"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HXZ37161.1"
                     /translation="MILEAFRRLKQAGVLGMNGRNAEYIMRCNPRSFFPLVDDKVLTK
                     KLAESFEIPTPMLYYVVDRYGDVPGFQAALRGICQFVVKPARGTGGSGIVLIQECTEK
                     GYVTQSGELISPQNLFYHIS"
BASE COUNT         2889 a         3873 c         4039 g         3185 t
ORIGIN      
        1 ggcctcctcc cattcttctg cgtggggagt cagctccttc tccacgaatt tccgcaccgc
       61 gtcccggaac agttcatgtt cttcgctgaa cataggatgt ctcatcgctc cactccttct
      121 tcgatttcta aagggacgca gattcacgca gatgcacgca gaaaaggcaa aaacccccaa
      181 aaaccttgtt gttatggctg caaccaaacc aaatcaaaag gaattcttgg tatcttattt
      241 tcaactacaa agtcctctat cggcgtttat ctgcgtgaat ctgcgtccca taattattct
      301 ttctttgaaa agcgctcccg cagctcgcgc ttcaacactt tgcccccgaa ctgggtcttg
      361 ggcagggctt caatgaaggc cactgaggtg ggcttcttat agctggccaa gtgggccttg
      421 cagaaatcga tcacctcaga ctcggtcagg tccatccccc ctttggggac gaccactgcc
      481 ttgatcgtct cgccccattt ttcatcggga atcccgatga tcaccgcttc ggcgaccttg
      541 ggatggcggg agaggacttc ctcgatctcc ttggaataaa cgttcagacc gccggtcttg
      601 atcatgtctt ttttccggtc cacgaaatag aggaacccgt catcgtcgta cttacccaaa
      661 tcaccggtgt ggagccaccc tcctcgcagg gtttgggcgg tctcctccgg gttatggtaa
      721 tactccttca tgaccgtagg ccccttgatg ataatttccc ccacctcccc gaccggccga
      781 tcgcggtctt ggtcatcgac gatccgtacc tccgtgcaca tgtaaggctt tcccacgctc
      841 cccgtctctt tcggccggtc cgtgggcttg agaatcgtga ccccggggga agcttcggtc
      901 agggcgtaac tcacgtaggt ctgagctcgg ggaaagagac gggtttctat ggcctgctgc
      961 atggtggggt gcaggaaccc ctgggaggag agatacatgc gcatggaagc ggtgtcgtaa
     1021 gccctcgatt ccatttcctg gagaagcctt ccccagatgg tggggttccc ggtcatggcc
     1081 gtgacttttt cctcttcgat gatctccaag cacttccggg cattgaactc cttggtcacg
     1141 atgaaggtcc cgccggcgta catgtaagcc agaaaccggc tcagcgcggc cgtatggaag
     1201 aggggcatgg ggtagtagac gacatcatct ggggtcggac tctggttttc cgtggtcacg
     1261 gagatgatgt tccacaggag cgatttgtgg gtgaggaccg ccccttttgg ggtcccggtg
     1321 gttccgccgg taaagaggat gaaggcctca tcctcttcct tcacttcgat ggggaagtcg
     1381 tcgggggact cgccagccat gagtttctcg tacgggcgcg cgaagaccgg gaggggatcc
     1441 ccgatgaaaa cccattcctg gacgtcgggg agggaagggc ggagtttctc cacccgggga
     1501 agaaaatcct gggtgtaaac gagtaatttg catcccgtga agacggcttg atagagaatg
     1561 tccttgtcca tgccccaggc gttgagggga acggtgatag ccccgatttt cagatgggca
     1621 agataggact ccatgagctc gattgaattg aaggaaaggg tggcgatccg gtctcctttc
     1681 cgcagcccca ggttcaggag ggcccgcgcc aagcggttgg accggtcgtt gagttcccgg
     1741 taggtccggc gcacgtctcc gcagcggatg gcggttttat gggggtgctt gcgggagttg
     1801 cgaaccactg aatagccaag ggtcatttcc atggcagcgg cctcctcgtt tcctgccctt
     1861 tggggtggcg gaatgacggg gatgacttga atcctgctct cagggcatgg gccaaaaaaa
     1921 ttccccctct cctgtatcag aagaggggga aatttgcaag aggatcggat cgggttaggt
     1981 aaggcctaaa gctttcaccc gatcccggcg atcttcttgg ccaggttctt tttgacctcc
     2041 aagtcggcct ggcggcggat cataatgcgc tgggcctggg gagggcccgc tccgtgcatg
     2101 gactcggtgc ggtagcccac cgcggccgtg cccagggtca ggttttcgat caggcgaagg
     2161 acccgcatgc ggtgctcggt gggcaccgaa gcgactccct tcaggtattt ttccacgaag
     2221 gggccggtgc gctcgctgcg caggtctttc tccgaaggca tggtcaccat caggccgccg
     2281 gcgatgtctt cggccaggcg cgcgatctcg taggggaatc gggtcacgtt ttgcttgcac
     2341 acattggcca ggagcatgtc gatctcaaag tttccggccc gagtggcgtg tccctccgag
     2401 gagcatgcca ggccgcagga gtatagggtc tcgttgagat ggaccatctc gatgatttta
     2461 tcgccgatgt gcgaggcctt ggcagctccg ttgtattctg cggccaggga ggcggccccg
     2521 atgaggacgt ctcccacgcc gaccttacag ccgccgtagc tctggcggtg atatccggcg
     2581 aaccgttcca caagggctcc gacgaaatcc gtctccccgc agaggaagac ccgatcccag
     2641 ggaaccaaga catcgtcgaa gatggccaaa acttcatgcc cgccgaattg atggtttcct
     2701 acgtctaggg aggatccctc catcttgcgc gtgtcgcagg actggcgtcc gtaaatgaag
     2761 aaaagaccct tggcatcggt aggtaccgca aaagagatgg cgtaatcctt ctcatcctcc
     2821 cggagggcca tggtaggcat gacgatcact tcctgggaat tgatcatgcc ggtctggtgg
     2881 gctttggccc cgcgcaccac cactccgtcc tttcgccgtt ccaccacgcg gaggaacata
     2941 tccgggtcgg cctgctggac cggtcgaagg ctccggtccc ccttcacgtc ggtcatggcc
     3001 ccgtccacca ccaggtcctc ctcctggacc cgctgcagga actctttgaa acgcctgtgg
     3061 taggaggtct tgtgcttttc gtccgcttca aaggtcgtgc tgtagagggc gttcatggcg
     3121 tccatgccca cgcagcgctg gaagcaagag gccgtttttt gtcccaggag ccgctgcatc
     3181 ttgactttat tcaccaggtc ctggctgctc tggtggatag cggtgaaacg gttgatggtc
     3241 ttgccggtca ggtgggaggt agtagtcatc caagccttat gctcgtcctg ctgggcgagc
     3301 tgaaaggtca tggtcacgga attgagggaa gggcggatga tgggatggtc caccgggttc
     3361 ttcacccgtt cccccagaag ataaacctcc aaattcaact tgcgaaggct ctcgatgtat
     3421 tgctctggcg tcttcatact tccttctttc cgggaaaagt tcgaagttac gagttcagag
     3481 ttcggaggaa aaaagcacaa cccgaacccc ggaactcgaa actagaaacc aggttatctc
     3541 atcaacgctc cgcccgccac catgcggttg atctggttgg ttccttcgta gatctgggtg
     3601 agcttggcat cgcgcatcag tttttccacg atgaaatcct tggtatagcc gtatcccccg
     3661 tggacctgaa ctgcatcggt ggtcacgcgc atggcggtat cggtggcgaa gaatttggcc
     3721 tgggccgcct cgcgggtgaa acgcttatgt tgatcggcca aggagcccgc gtaccaggtc
     3781 aggaggcggg aggcttccac ggctgcggcc atatcggcga gcatgaaatt caaaccttgg
     3841 tgagttccga tggggcgtcc gaactggacc cgggtcttgg cgtaggagag ggctgcctcg
     3901 aaagccgcgc gggacaggcc gaggccgacc gtggcgatgt tcacccggcc ttcgtccaga
     3961 gcttccatgg cccatttgaa cccctctccc tcgttcccca agcggttttc ccggggcacg
     4021 accacgttct ccagaatgac ctcggctacg ttcagggacc gctgtcccat cttgtcttcc
     4081 accttcccga tggaaaggcc tggagactcg cggggaacga caaaaccgca gatccccttg
     4141 gtccctttgg agggatcgat ggtggcaaag atggaataga gggcggcata gctggcgttg
     4201 gtgataaagc atttctggcc attcagaacg taatggtctc cttcgagttt ggcccgggtc
     4261 ttcaggcctc ctgcgtcgga cccggcttcc cgctcggtca gcccgaaaga ggccgttttc
     4321 cccgactggt tcagggggag gaggaagcgc cgtttcagct catccgaccc accagcaagg
     4381 atgggaccgc aggccaggga gttgatcccg atcattccgc tgatggccac gcaggcggca
     4441 ttcatttctt cgttgatgat ggaagaatcc accagcccca gcccctgtcc gccgtactgt
     4501 ttggggcagg ttaggttcat caagccggcg gcgtgggcct tctggattat atctccagga
     4561 aacctcgggt tccggtcgta atccaaggcg gcgggggcga tctctttggc ggcaaagtcc
     4621 cgccccgttt tctgaaaggc tttttgctgc tcgctcaggg caaaatcaat catgcgggtc
     4681 ctcctccttt ttccgaatgg aatgatggaa ccatggaata ttcagggttc aaatgccggg
     4741 aattggacat tccttcgatt tctcattttc cccagcatcg aaataaaata acatttttat
     4801 caaaaattct ttgaactgtc cagaaagatt gttaccgggc cgaagttgac cagctccacc
     4861 tccataatgg cctgaaagat acccgtggcc acctttaacc cccgggtcct agcagcaccc
     4921 acgaaataat cgtatagctt ctgggcatcg tctggagcgc tggcatcggt aaaggaagga
     4981 cggcggcctt tacgacagtc cgcatagagg gtaaattggg atatgaccaa tagttccccg
     5041 ccgacctcca gaagggaaag attcatcttc ccctgggcgt cctcaaaaat gcgcaaatgg
     5101 acaatcttgt ccaaaaggta atccgcctct tgggaggtat ctccccggcc tacccccaaa
     5161 agaaccagaa ggcctggccc gatggaaccg gtgctttgcc cattgacccg cacctgcgct
     5221 cgactgactc tttgaatcac agctcgcata gaagcccgca attcttctgg ttcaaacttt
     5281 ttgcggaagg attgcagagg tcaggttcca gatcataaat taaccccttg gggggccttt
     5341 caagctgaaa tcttaaacct gtaatcggaa ccccaatttc tatttcccct tctccttgat
     5401 ggagccttcc aggtccagcg aagtcaggga ggcgaccatc tgccgggggg aagcggaagg
     5461 atcatccatc caccgcagca gtgggttcat ggctttctga aattcgggca tctgctcatt
     5521 tgtgagggcg ggggcccggg gggacacctc ccttaaaggg ttgcggggca ctccattccg
     5581 cagcaggcga aaatccagat ggggcccggt ggaaagtccc gtggaaccca gataaccaat
     5641 gatttgcttc tggtgaaccg gtttgccctt gctgatcccc ggcccatacc cgctgagatg
     5701 cccgtacatg gattggtacc ccttggggtg gcggacgacc acctgcttcc cgtacccgcc
     5761 gttccatccg caggagatca cggtcccgtc ggccaccgcc cagaccggag ttccctgggg
     5821 cgcggcgtaa tcaatcccca agtgggggcg gtattcgccc aggatcggat ggcggcgcga
     5881 ttgggaatag ccggaagaaa tccgggtgaa tttcaggggc gaccggagga aagccttgcg
     5941 caacgattct ccttgaggag tgaaatagtc gccccgctta cccggctctt ggaagtagac
     6001 ggcttggtgg tttcgcgctt cccgttggta ttcggcatag aggatgcgtc cgtaacggac
     6061 aaagacatcc ccggtaaaat acttttccac caccatacag aaccggtcgc cgggctgagg
     6121 gtccacgtga aaatcaatct cccaggcgaa gatgtcggca aaatccatga ttaaggaatc
     6181 cttctccccc agccggtcca tggcctcgaa gaggctggaa gtgatttccc cggagacgcg
     6241 ggcccagtag gcattcaccg gaacttcttt tttactggcg atccattctc cggcggaggt
     6301 cagggaaagc tggtagatat ccaaggggcc gaccgtgtag gtgaggtcca aaaactgccc
     6361 ctgggggctc agtcggactt cgaaagaatc accgggcttg acccgccgga ggttgatgac
     6421 ctctttcaga tgccggcaga tgatttccac caagtctttg gggagatcct gggcgcggag
     6481 ggcggaagag agattctgcc ccttgaaaat ttggccggaa atcactcggt cgggcagggg
     6541 gggaggcggg gatgcggtct gaggaagtcc caccccggct tctgttgacg ggtttctttc
     6601 caatccggaa aagaaaaaga aacctgccgc cacgaggaag gctccggcca gagaaagagc
     6661 ccagatgaac cataaccttt ttctcctggg ggaaaggatt tcgtcgtttt ttttgtcgat
     6721 catgatttcc gggatcgatt gattctccgc ttcttgaccc actatacaat cgggagacgg
     6781 gcttttcaag gtgaaatgag aatgttcacc ggaaggtcgt gaccaccatg gccccggaga
     6841 gaaggatgag cacggcgatg gtgacgaaag agatgaggtt caagacccat ccgttggtga
     6901 atttccccat gattctccgg tcgttgacca agatgagcat gaagatgagg ataaccggca
     6961 ggagaacccc gttgatcacc tgggagtaga acatgatggg aatgaggggg attttaggga
     7021 agagaatgac cgcggctccc aggatgatca tgagggaata gaggccgtaa aactggggag
     7081 cttcggcgaa tttccggttg acgctcgctt cccacccgaa ggcctcacag atggtgtaag
     7141 ccgtggaaag gggaagaatg gaagccgcaa aaagagaagc attgagaagc ccgaaggcaa
     7201 agagccaggt gcagtacttc ccggccaatg gggccagagc cagggcggcg tctttggcag
     7261 tttcaatctt gatcccggcc tggaaaatgg tgagcgcgca gagcatgatg atgaagaagg
     7321 ccacgatgtt gacgatgatc gaaccgaaga ccacgtccat ccgggcgtac tggtaatctt
     7381 cggctttcaa ccctttgtcc accaccgagg actggaggta gaactgcatc cagggggcga
     7441 tggtcgtgcc cacgacgccc acggccatgg tcagatattc gggggtcaac tccatagtac
     7501 ccagagtggg ggtgaggaaa tgggcggtga tctcccccca gtccggtttc accatgaacc
     7561 cggatacgat gtaggaaatg taaaagagac aagccaccag gaaagccttt tccaccgact
     7621 tgtagctccc cttcaccacc agccaccaga caaagaaagc gctcaggggg acggagaggt
     7681 acttgctcac cccgaagatt tccaggctgg ccgcaactcc ggcaaactcg gatatggtgt
     7741 ttcccaggtt ggtcaggaag agggcgatca tcaagtagaa ggtagcccgg gcgccgaaac
     7801 gttcccggat caggtcggag aggcccttgc cggagacgac ccccatacgg gcgcacatct
     7861 cctggatgac gatcagggcc agggtgatgg ggaagagggt ccagaggagg gagagaccat
     7921 attctgaacc ggccagggaa tatgtggtga tgccccccgc atcgttgtcc acgttggaag
     7981 tgatgatccc cgggccaaaa agggcgaaaa ataccgccag ggtcttccag aaccccttgc
     8041 gggcaatctc cttttcaggc ccccctgaag ctcccggggg agcgggaagg gtgccattgt
     8101 tgttggaaac caaggttgtg gaccctcatc ttccgtggaa ttcatttccc taactcaggg
     8161 gccaccactt ccaggaggct cttgaaggga atgacccctt tgatgtggtt ctcttcgtcc
     8221 actacgggga ttgccttgag gccgtatttg gcaaaaagtt tggcaatctc ttctttttca
     8281 tcgtccaggc ccacattcac tacccgtgag tccatcatgt cctccgcgcg ggtctgggga
     8341 gaggcgatca aaaggtcccg caggctgatc actcccagga ggtgctcttc cgggtcggcc
     8401 acgtaagcgt aatcgatgac gtccaggtta tccgcttcct gcctaaggag ttccagcgcc
     8461 tctcgggccg tggcccgagg gggcagggtg gcaaaggcgg tggtcatcag tccccccgcc
     8521 ttctcctccg ggtgttccaa aagccccttc acgtcctcgg cgatctcttt ctccatctct
     8581 ttcaggatcc cctcggcctt ctccttgggc aggtcgccga gaaggtccgc cgcttcgctg
     8641 ggcgacatct cttcgatgat gtccgaggcc ttggccggat tcagattctc gatgagccgc
     8701 acctggatct tgggatcggt ctcttcgagg gcttcggcgg cggtctccac atccagggac
     8761 tggaagacct ctgtcctctt atggatgtcc aggtcctcga tgatatccgc caggtccgcg
     8821 gggtgcagca gggacaggcg gttctgggcg atcttcagcc gcagaaggtc cggggaagag
     8881 aggggctgga cgaacctcca ggggatgaaa tgatctgcca ggtgataatc gaagaaccat
     8941 tgaaagaatt tgtccgtcgc cttctccagg cccacccgcc gcatgagccc ccggaacccc
     9001 acgtccacat ggacgagaaa caggttctga ttgaccttca gcaggtggag gtcgttgacc
     9061 cgaaggattt tggccccgtg ggtgtctacg atctgcttgt ccaggaggga ctccttgagc
     9121 agaagctctc cttcgttccg gtttccggat gagacatcgg gggaaacggg agacgaagta
     9181 acgaccgggc cgaagatatc ggtgactttg ttccagggaa gacgggcagg ggattgggca
     9241 cccctgggca gatagagaag ggcggtaacc ggggggtata aatcggccac gacggctacc
     9301 aggtccaaga cctttccctg gaactggccc gcagaatcca cgatgctctt tcccaaaacc
     9361 tgactcaaaa ataaaaaaac ctgagagttg ggggaagacg ccatacaaaa acctccaggt
     9421 tacaattttt tataacactc ttaagaggat ttgaaaagtt ttttctgttt tggggatgaa
     9481 aatagaaagc ctttagttta tactcattta aatatggaag gatgagcttc cggtcctgac
     9541 caatcggaaa tcgccctaga aagcgacccg tgataaaacc catcccattg aaacccttct
     9601 tttctcgcca cctatctccg gagcgtatct ttatctcggg atttgccggt tatatcctcc
     9661 tgggagctct gtttctatgg ctgccccttt ccgcggcgaa aaaccctgtt cccttcatcg
     9721 atgctctttt cacctccacc tccgccgtgt gcgtgaccgg gctggcctcg atcgatatcg
     9781 ggacgagctt ctccttcttc ggtcaggtgg ttaccatcct tctttttcag gtgggcggcc
     9841 tggggatcat caccttttcc acgattttct tcgtcctgat ggggcggggc atttccttta
     9901 aggggaggga gatcgtgcaa tcgaccttcc ttcacactcc ccgggcggac ttctttgcca
     9961 tcctgaaatc ggtcctgtgg ttcaccctcg tctttgaaac cattgggatc cttttcctgt
    10021 ggtatcgttt ttgttacgat ttccccgtgg gccatgcctt ctatcaagcc gtttaccacg
    10081 ccatctccgc cttcaacaac tgcggctttt cccttttccc cgacaacctg gtgcgttacc
    10141 agggagactt catcgtcaac ctgacggtaa tgaccctaat cgtggtggga gggatcggtt
    10201 ttgtggttca gcatgaggtt ttcgccaagc ttcgaggaag acagagaaag ctttcagtgc
    10261 acacccggat cgtcctcctg gcgaccggaa ttctgattct ggggggcgcg attctcatct
    10321 atatttttga gaggaatcac atcctctacg ggatgcccgg gcagaaccaa atcctcgcga
    10381 tcttctttca atcggtggtt ccccgcacgg ccggcttcaa caccgtggac atcggattcc
    10441 tgaccaatgc cactatcctg ctcatgatca tcctcatgtt catcggggcc tcccccggat
    10501 cgacgggggg aggggtgaag accaccagcg cctccatcct tttcctcctc atgtgggccc
    10561 ggctgcgggg aaacgaggag gtaaacgtct tcaagaggac catccccagg gaggtcgtct
    10621 cccggaccat cgggattatt ttcgcttcgg gtttttccgt gtccttggtc acttctgcac
    10681 ttctgttctt ctcctcgggg gacctgggtc cattggagag ccgtcattat ttcgtggaat
    10741 acctgttcga aacggtttcg gccttcggaa ccgtgggact atccatgggg gtgaccccgg
    10801 acttgaacgt agctcagaag ctggccatta ttctgatgat gtacgccgga cgggtcggcc
    10861 cgctgaccct ggccttttcg ttctcccgca aggccttggc caagagcctg acttatgcag
    10921 aagagggagt catggtgggc taaggaggga ttcgcgtgaa gcgagtagtg gtcgtgggac
    10981 tgggaatctt cggcttcaac attgccaagg acctttttga aaacggtttt gaggtgattg
    11041 ccatcgacaa aaataaggac gtggtcaacc ggatccggga cttttccacc aaagccgtgc
    11101 tggccgatgc cacagacagg gaactgctgg aaatgatcgg aatccagccc gacgaccttg
    11161 tgatcatctc tttcggggag gacctggcgg ccagcaccct tctgaccctc cacctaaagg
    11221 aaatgaacat taaaagtatt ctcgtcaaag ccccgaatga ggaccacaag cggatcctgg
    11281 aaaaagtggg ggccaccgag gtcatcatcc cggagaaggc gatggccagt aaggtggcca
    11341 aaggtttgat ctctcccaat gttttggact tcctgccgat atcggaggat ttcactattt
    11401 ctgaggtcgc tcccccggcc gggttcttag ggaagactct ggccgatctc cgcctccggg
    11461 gaaaatacca cgtcgaggtc attgccatcc gggatgtatt gtcggataaa attcaactcg
    11521 ttccccgggc ggacttcgtg attaaagaca gcgatattct gatcgtcatc ggcaaagaag
    11581 cggatatcca gaagatccag tgagttggaa agtatgaaag cccttgcgtt ttttgtcctg
    11641 cttttcttcg gaggggggat gcaacctttg atggcccaag aaaaaatcac cctcggcctg
    11701 gtggaggaaa ttatcctcct gccctggatg gtaaaaattc cggcccgcat cgataccggg
    11761 gccgccgtat cttctctcga cgtgcgcggg ctgacggtga agaggaaaac ggcggaattc
    11821 aacctttcgc caaagtacag tggtgcgcga atcagcgtgc cggtcgtggg ctggcattgg
    11881 gtccgttcgg cggaatcccg ggagaagaga ccgatcgtag agatcgatct ctgtgtggga
    11941 cccaaaaaac tgcgggcccg ggtcaattta aatgaccggt cccaggttca gtatcccttt
    12001 ctcatcggcc ggaatatcct gaaagagaat ttcgtcgtgg attgcatgaa ggagtattgc
    12061 aacccaccca gttgtccgga ccttccccag aaatgaaacg ccccgcgctc tggctggctc
    12121 tttctctctt cctgctgact gcggccctcc ttttctatcg cattgtctgg cttggatacc
    12181 ctgttttccc aacggctccg ggaaggacct gggagctgac gatcaacgct cgggttatgt
    12241 cggatacggg agaagccaga gtcctcctcg ggcttcccac ggagaatccg ggccggatgc
    12301 tgttggaaga gaggatctcc tcagggtcct tgagcctgag cctcgcacgg gaagggccca
    12361 accggatcgg aatttggtca ggtcccgtag agttggaaag ccaggaaatc acctaccggg
    12421 cgacaatcct gatccgtccc cgccaaagac ctccagagca cccctcgggg atcggacctt
    12481 atccggaccg atttgaccgc gaagagacag ccctggcaga acgtttagcc ggacggtgga
    12541 agggcctccc gccttcccaa ctggtccggg ccattgccgc aactgcgaaa ggggaatgga
    12601 agaatcctcc gccggagatg aaggacctcg atggctggaa ggttctgagc cggacctatg
    12661 gccgtgagga gtccctgctt ctcctcttgc gggcggccaa tctgcccgca cggctggtgg
    12721 agggacttcc actcgtggag agcatccaaa accagctcct tacctgggtg gaaagctgga
    12781 acgggaatca ttgggaaatc attcttccgg agaggggaga ggtcgtatcg aaccccgcgg
    12841 gttatctgcc gctctcggtg ggcggcgttc ccgcgctccg ggtttcaatc ggcgcacgag
    12901 cagatatccg ctggagccta agccggcagg tggtcagcca gtggagcctc cactgggaac
    12961 ggatccggcg ctccaaccgt ttcctggacc agtggtccct ttttcgcctc cccccggagt
    13021 ttcaaaggac cttccgtgtt cttctcctgg tgcccatcgg ggcgctaatc atcggtttcc
    13081 tgcgtaacat gatcggattt cccacctttg gtattttcat gcccatgctc atggccctgg
    13141 ccttccggag cacgggcctg gcctatggcc tgagtatctt tggcggcgtc cttctggtag
    13201 gctatgccgt gcgccgcggg ctggataaat tgcggcttct cctggtaccc cgcatgtcgg
    13261 tcatcctgac cctggtgatt gcctgtttta cctttatcgc cctggtggga aataagtttg
    13321 gcatgcggga gttcatggcc gtggggttgc ttccctttgt catcttgact atggtaattg
    13381 aaaggttctt tgtcctcgtt gaggaagcgg gaatcagaga aggggtgaag accgctgccg
    13441 gaagcgcggc tgtttccatc attgcctatg gaattattag ctgggaaccg cttcagctga
    13501 cttttttcgt ctaccccgaa cttttggctg ccgtggccgc ggtccaaatc cttctgggga
    13561 ggtataccgg ttatcgcttg tccgaacttt tcaggtttcg tggggtgggg ggttcttcat
    13621 gatccttgag gccttccgcc gcttgaagca agccggggta ttggggatga atggccggaa
    13681 cgctgaatac atcatgcgct gcaatccgcg atcattcttc cctctcgtgg atgataaagt
    13741 attgactaaa aaattggcgg aatcttttga aattcctact ccgatgcttt actacgtggt
    13801 tgatcgatat ggagacgtcc cgggcttcca ggcagccctt cgcggcattt gtcaatttgt
    13861 cgtgaagcct gcccggggga ctgggggaag cggaatcgtc ctcatccaag aatgcacgga
    13921 aaaggggtat gtgacccaga gcggggagct catctctccg cagaacctct tttatcatat
    13981 ttccga
//
LOCUS       DASPRR010000383         6064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_895942, whole genome shotgun sequence.
ACCESSION   DASPRR010000383 DASPRR010000000
VERSION     DASPRR010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895942"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1120
                     /locus_tag="VEL68_14170"
     CDS             227..1120
                     /locus_tag="VEL68_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37162.1"
                     /translation="MEFWLFLQLLINGIIVGSLYALLGISFTAIFAPTKIFHFMHASV
                     YVFAGYFLYQFAMGWGWPVSVSILAAVLLAGVLGVLAERVIYSPLRRMGAGSMEMLLS
                     SFGGYVILQNLILLIWKSDPRTVKIPEVLKNGIPVGLLTFTPLDILTVITSGAALAGV
                     TLFMRRTKVGKAMRAVENDPLGATITGIPTEWVRLVSFFLGSVLVAVAVTFHVVDKGL
                     EPTIGVNAVLVATVAMIVGGVGSYGGAALAGFMIGIAENLGIWQIPSEWKNTITFCVL
                     VLFILFRPRGFFGHRMTRYEG"
     gene            1124..2005
                     /locus_tag="VEL68_14175"
     CDS             1124..2005
                     /locus_tag="VEL68_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37163.1"
                     /translation="MIYLIYVITLIAVYGVLTVSLNLSVGYTGILSLAQAAFMGIGAY
                     AAAILLTEWGFNFFTTLLVGMAVAGLVSWGLAWATLRLKGDYYVIASFGFQIIIYNIF
                     LNWISLTRGPFGIRHIPRPEVFGFSFLGPPSYALFTLGFLALCVWIARRVGESPYGKV
                     LRAIREDEAAASSLGKDVNRYKISIFTLTAVLASVGGALYAGLITYIDPFTFTIHESI
                     FILALVIIGGAGNVYGSVIGATVLISLPEVLRFFDVPTAVASPIREILYGTLMIIFLR
                     LRPKGLLPERITSGVAR"
     gene            2005..2772
                     /locus_tag="VEL68_14180"
     CDS             2005..2772
                     /locus_tag="VEL68_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37164.1"
                     /translation="MDRDKSFFQVDRLSKAFGGLQAVAEVSFLVEKGSLTSLIGPNGA
                     GKTTVFDLISGFLPADDGRIVFKGRDLAQQKPHDIVSLGMARTFQHLGLFNQLSVWDN
                     VFLGLPGKRRESLWGVFLPRPMLRREERALEERTREILDYLGLTEKAGERVANLSWGD
                     QKLVSIGRLLATDGEFLLLDEPASGLSDEQIEHLKSLLRGLVSRGKTILLIDHNMEAV
                     MDISDWVIVLNFGKIIAEGNPAEILANEEVVRIYLGV"
     gene            2775..3497
                     /locus_tag="VEL68_14185"
     CDS             2775..3497
                     /locus_tag="VEL68_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011849528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37165.1"
                     /translation="MGKIHPLLSVDHLSTGYSKKTVLEGVSLHVKEGERIAVVGRNGA
                     GKSTLLLTLLGVVKAWGGHVLYLGKESTHASPEERVRSGMALVPQGGRVFANLMVREN
                     LELGAYVTRDLKAVGSSMELVYQTFPILRTRATQEAGTMSGGERQMLAIGRAMMSQPK
                     LLMLDEPSLGLAPLVVKELMEKVHGLSTALETTILIVEQNVRAAFKIVERVYVLKLGK
                     IVFEEKPETLFQDERLRKAYLA"
     gene            3569..4261
                     /locus_tag="VEL68_14190"
     CDS             3569..4261
                     /locus_tag="VEL68_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HXZ37166.1"
                     /translation="MNILEAIRIRRSIRSFKPTPVPKKILEEVLDNSRWAPSGSNTQP
                     WEFAVLGGKAMEEINVLLAQKVRTEWDTDRLTFKTFTPDIPFPEFPEPYRQRAINARN
                     RIDSHQFPAGTPGIDAKRAAYLLYGARFYGAPNAIIVYTERSICPKAILDIGLLAQTI
                     ALAALAYDLGTCLMTTPVAWPEIVRGPLGIPESKVIALAIAIGYPETEAKVNNFDRTR
                     EPLDALTRWYGV"
     gene            4791..>6064
                     /locus_tag="VEL68_14195"
     CDS             4791..>6064
                     /locus_tag="VEL68_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HXZ37167.1"
                     /translation="MNDDRKSPVTGGANKQATGRGTSNRDWWPNQLNLKILHQHSSKS
                     NPMGEGFDYAKEFKRLDLAAVKKDLQVLMTDSQDWWPADFGHYGPLFIRMAWHSTGTY
                     RTGDGRGGGGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGRKISWADLMVLAGNV
                     ALETMGFKTFGFGGGREDVWEPDQDVYWGAEKTWLGSDKRYSGERDLENPLAAVQMGL
                     IYVNPEGPNGNPDPIAAAKDIREIFARMAMNDEETVALIAGGHTFGKTHGAGPASHVG
                     PEPEAAGIEEQGLGWKSSFGTGKGGDAITSGLEVTWTPTPTKWGNNFFRNLFGYEWEL
                     TKSPAGAHQWKPKGGAGAGTVPDAHDPSKRHAPGMLTTDLSLRFDPAYEKISRCFMEN
                     PGQFADAFARAWFKLTHRDMGPRARYLGPEVPA"
BASE COUNT         1235 a         1709 c         1782 g         1338 t
ORIGIN      
        1 tggggtctct ggcccggtga cctttgatca ggacgggatc accacaaccg gggccgtgat
       61 tcgtgagttc cagaagggcg cttttgtaga tgtgaaatgg aaatgaggct cggcacccgg
      121 agaaatcgta ggggcgattc atgtcggaga ctcgactcta cgagaatcgc ccctacggcc
      181 ttcatcctgc cctcgcgcac gataggccac ctctaaaggg aaatccatgg agttctggct
      241 ttttcttcag ctactgatca acgggatcat tgtgggaagc ctctatgccc tgttgggaat
      301 cagcttcacc gccatattcg cccccaccaa gatcttccac ttcatgcatg ccagcgtcta
      361 tgtttttgcc ggctatttcc tttaccaatt tgccatggga tgggggtggc cggtttccgt
      421 ttccattctg gccgcggtgc tgctggcagg ggtcctgggg gtcctcgccg agcgggtcat
      481 ctattctccc ctccggagaa tgggcgcggg atccatggaa atgctgctga gttctttcgg
      541 ggggtacgtc attctccaga atctgatttt gctcatctgg aaaagcgacc cccggacggt
      601 gaaaattccc gaggttttaa aaaacgggat tcccgtgggg ctcctcactt ttactcccct
      661 ggatattctg accgtcataa cctctggagc tgcccttgct ggcgtgaccc ttttcatgcg
      721 ccgtaccaag gtcggaaagg ccatgcgcgc cgtggaaaat gatcctctgg gcgcaacgat
      781 caccggcatt cctacggaat gggtccggct ggtgagtttt tttctcggct ccgtcctagt
      841 ggcggtggcg gtcaccttcc acgtggtcga taagggtctg gagccgacaa tcggggtcaa
      901 cgccgttctg gtggccacgg tggcgatgat cgtgggcggc gtgggttcgt acggcggcgc
      961 ggccctcgcc ggattcatga tcgggatcgc ggaaaatctg gggatctggc agattccttc
     1021 cgaatggaag aacaccatta ccttttgcgt actggtcttg ttcattctct tccggccccg
     1081 ggggtttttc gggcaccgga tgactcggta tgaagggtag gacgtgatct atcttattta
     1141 tgtgatcacc ctgatcgcgg tctatggcgt actcaccgtt tccttaaatc tttccgtcgg
     1201 gtataccggc atcctctccc tggcccaggc cgctttcatg ggaattgggg cctacgccgc
     1261 ggcgatcctg ctcaccgagt gggggttcaa tttttttacg acccttctgg tgggcatggc
     1321 cgtcgccggc ctggtgagct gggggctggc gtgggcgacc ctccggttga agggggatta
     1381 ctatgtgatc gcctccttcg gctttcagat catcatctac aacatttttc tcaactggat
     1441 cagcctgact cggggccctt tcggcatccg ccacattccc cgtccggagg tcttcggatt
     1501 ttctttcctg gggcccccga gctacgccct tttcaccctg ggattcctgg cgctgtgtgt
     1561 atggatagcg cggcgcgtgg gcgagtcccc gtacgggaag gtcctccggg ccatccggga
     1621 ggacgaggcc gctgcgtcct ccctcggcaa ggacgtgaac cggtacaaga tttcgatctt
     1681 cacgctgacc gccgtgctcg ccagcgtggg gggggcctta tacgccggcc tgatcaccta
     1741 catcgatccc tttacgttta ccatccacga atcgatcttc atcctggccc tggtcattat
     1801 cgggggggca ggaaacgtgt atgggtcggt gatcggagcg acagttctga tttccctgcc
     1861 ggaggtgctg cgcttcttcg acgtccccac cgcggtggcc tctcccatcc gggagatcct
     1921 gtacggaacg ctcatgatca ttttccttcg cctgcgtccc aaggggttgc tgccggagcg
     1981 gatcacctcg ggggtggcgc gctgatggac cgggacaaat catttttcca ggtggaccgc
     2041 ctgtcgaaag ctttcggggg tcttcaggcg gtcgcggagg tttcgtttct cgtggagaaa
     2101 ggaagcctca ccagcctgat cggccccaac ggggcgggga agaccaccgt gtttgacctg
     2161 atctccgggt tcttgcccgc ggatgatggc cggatcgtgt ttaaaggcag ggatttggcc
     2221 cagcaaaagc ctcatgacat cgtttccctg ggaatggccc ggaccttcca gcatctcggc
     2281 ctcttcaacc agctctccgt ctgggacaat gtcttcctcg gcttgccggg caagcgccgg
     2341 gaaagcctgt ggggcgtctt cctcccccgg ccgatgctgc gtcgggaaga acgggcgttg
     2401 gaggaacgga cccgcgaaat cctcgattac ctgggtctca ccgagaaggc cggagagcgg
     2461 gtggcaaacc tctcctgggg cgaccagaag ctggtctcca tcggccggct gctggccacg
     2521 gacggagaat tccttctgct ggacgagccg gcctccggcc tctccgacga gcagatcgaa
     2581 cacctcaagt cgcttctccg ggggctggtt tccaggggaa agacgatcct gctcatcgac
     2641 cacaatatgg aggcggtgat ggacatttcg gattgggtga ttgtcctcaa ttttggaaag
     2701 atcatcgccg aaggaaaccc ggccgagatt ctggcgaacg aagaagtggt ccggatttac
     2761 ctgggggtgt agcgttggga aagattcatc cgcttctgtc cgttgatcat ctctcgacgg
     2821 ggtattccaa aaaaaccgtg ctcgaagggg tttccctgca tgtcaaggag ggggaaagga
     2881 tcgccgtcgt ggggcggaac ggcgccggga aaagtacttt gctgctgacc cttctgggag
     2941 tggtcaaggc ctggggaggc catgtcctct acctgggaaa agagtccacc catgcttctc
     3001 cggaggagcg ggtacgctcg ggaatggccc tggtacccca ggggggaaga gtttttgcga
     3061 acttgatggt ccgggagaac ctggagttgg gggcttatgt gacgcgggac ctcaaagccg
     3121 taggttcatc catggagctt gtctaccaaa ctttcccgat ccttcgcacc cgggccaccc
     3181 aggaagccgg aaccatgagc gggggagagc gtcagatgct ggccatcggc cgggccatga
     3241 tgtcccagcc gaaactcctg atgttggacg aaccctccct gggactcgct ccgctggtgg
     3301 tcaaagaact catggagaag gttcacggtt tgagcacggc cttggagacg acgattctga
     3361 tcgtggagca gaatgtccgg gcggccttca agatcgtcga acgggtctac gtcctcaaac
     3421 tggggaaaat cgtgttcgaa gaaaagccgg agaccctctt ccaggatgaa cggctgagga
     3481 aagcctattt ggcctgaaga gaatagacct attgggtctg tctggtctat ttcgcctatt
     3541 gggtctgata tcgaatcagg aggacatcat gaatattctc gaggccatcc gtatccgaag
     3601 gtccattcgc agtttcaaac cgactccggt gcccaaaaaa atcttggaag aggtcttaga
     3661 caacagccga tgggcgccct ccgggagcaa cactcaaccg tgggagtttg ccgtcctggg
     3721 gggcaaagcc atggaggaga taaatgttct tctcgcccag aaggtgagga ccgaatggga
     3781 taccgatcgc ctgactttta agactttcac cccggacatt ccgtttcccg agtttcccga
     3841 gccctatcgc cagcgggcga taaatgctag aaaccgtatc gacagtcacc agttccctgc
     3901 cgggacaccg gggattgacg ccaagagggc cgcttatctg ctctatggag cgcgcttcta
     3961 cggagccccc aatgccatca ttgtctacac cgaaaggtcc atctgcccca aggccatctt
     4021 ggatatcggc ctgctggcgc agacgattgc cctggccgct cttgcctatg acctgggtac
     4081 ctgcctcatg accactccgg tcgcctggcc cgagattgtg agaggacccc tcggcattcc
     4141 cgaatccaag gtgatcgccc tggccatcgc catcggttac cccgaaacgg aggctaaagt
     4201 caacaacttc gaccggacgc gcgagccgct ggatgctctc acccggtggt acggggtcta
     4261 acctaccgcc attgttgcag aagtacccgc tgggagcacg tcggctattc cctcggctgc
     4321 acagatttca acgataaaat tatttaactt ttggacgaat ttctttcgtt atcatctgaa
     4381 aggacaactc aaaaagcatt acttcccgaa atgttgtttt tctaattttc cccatccact
     4441 caacatttca aagcaaaatc atcagtctct tccccgggtg agacagcatt tccaatcgaa
     4501 gttccaactt gtggcatacg cctcctctac atacatccat cttttctcaa aaaatacaat
     4561 cttttttggg acaaaatacg gtcttcaatg tatttgattc tctattcata taatttatat
     4621 ctggaaaaga aagatgatag tcacacctta tccaatccat taagggggat aaaccatgga
     4681 aaagacagtc gtgttaattg tctaattttg tattagaact agatgggcaa ggaaaactat
     4741 attcgtagca ccatagtgga taaaatcaga cgacgatagg aggtaacgtt atgaatgacg
     4801 acagaaagtc cccggtaacg ggtggagcaa acaaacaagc aaccggcaga ggcacgtcca
     4861 accgagactg gtggcccaat cagctgaacc tcaagatcct gcaccagcat tcctccaaat
     4921 ccaatccgat gggtgagggt tttgactacg ctaaagagtt caagcgcctc gacctggcgg
     4981 ccgtgaagaa ggatctgcag gtgctgatga ccgactcgca ggactggtgg ccggcggact
     5041 tcggccacta cgggcctttg ttcatccgta tggcgtggca cagcaccggc acctaccgca
     5101 ccggtgacgg ccgtggcggt ggtggcaggg gccagcagcg cttcgcgccc ctcaacagtt
     5161 ggccggacaa cgtcagcctg gacaaggcgc gccgattgct ctggccgatc aagcagaagt
     5221 acggccggaa gatttcctgg gccgacctca tggtcctcgc tggcaacgtc gccctggaaa
     5281 cgatgggatt caagaccttc ggttttggtg gcggtcgcga ggacgtctgg gaaccggatc
     5341 aggacgtcta ctggggcgcc gagaagacat ggctgggtag cgacaagcgc tattctggag
     5401 agcgggacct cgaaaatccg ctagctgccg tgcagatggg tctaatttac gtcaacccgg
     5461 aaggcccgaa cggcaacccg gacccgatcg cggcagccaa ggatatccgt gagatcttcg
     5521 ctcgcatggc gatgaacgac gaagagacgg ttgcgctcat cgccggtggt cacaccttcg
     5581 gcaagaccca cggcgccggc cctgcgtccc atgtggggcc tgagccggaa gcggccggta
     5641 tcgaggagca aggcctcggt tggaagagca gcttcggcac cggcaaaggc ggtgacgcga
     5701 tcaccagcgg cctggaggtc acctggaccc ccacgccaac aaagtggggc aacaacttct
     5761 tcaggaacct gttcggctac gaatgggaat tgacgaagag cccggccggt gcgcatcagt
     5821 ggaagccgaa gggtggcgca ggcgccggta ccgtgccgga tgcgcacgac ccgtcaaagc
     5881 gtcacgcgcc aggcatgctg accactgacc tttccttgag gttcgacccc gcctacgaaa
     5941 agatttcacg gtgctttatg gagaatccgg gtcagttcgc agacgcgttt gcccgggcgt
     6001 ggttcaagct gacgcaccgc gacatgggcc cgcgcgcacg ctatctcggc ccggaggttc
     6061 ctgc
//
LOCUS       DASPRR010000384         6791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_896054, whole genome shotgun sequence.
ACCESSION   DASPRR010000384 DASPRR010000000
VERSION     DASPRR010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896054"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VEL68_14200"
     CDS             <1..971
                     /locus_tag="VEL68_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXZ37168.1"
                     /translation="GGCATMAPSGKMYTLKGSPQTAHTGFFDATIPPVLTVNSGDTVT
                     LSTMWLMDGQLRCGMTLEELIAKRQTYIDRKMGPHTLTGPIFVSGAEPGDVLEVRIQK
                     LIPASCGVNYHFPAKMNIGGLPEDFPIGQFKTLQLDVQKMETTFAPGIVIPLKPFLGV
                     MGVAPKPGEKRPAAVPEYFGANMDLKELVAGTTLYLPVSAKGALFSTGDAHAGQGDGE
                     VNVTAIETAMEEAVLQFVVRKDLKLERPMAETPTHWIAMGFHKDLNEAVKIALRDAIQ
                     FISKTKGLTPDDAYSLSSMAVDLRVTQIVDGNKGIHAMIPKAIFKK"
     gene            complement(1159..1980)
                     /locus_tag="VEL68_14205"
     CDS             complement(1159..1980)
                     /locus_tag="VEL68_14205"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HXZ37169.1"
                     /translation="MAVITVSRQVGSLGTEIARETARFLNYEYVDKEKLEQLLKGFGF
                     PEPEMEKFDEKKPPFWDSLSLERRKFLHATQAVLYDLASKGRVVIVGRGGQVLLKEVP
                     GTLHLRIIAPFEKRVKRMVETGGFDDKQAARFLRQSDQDSFGYIQTFFHANWDDPSVY
                     DLIINTADLSVETASKIITDAVLSPDVTGKENLAREKLGELALVQKVEARLMDVLGTD
                     VRNVEVGAKKGTVLLRGMVISARSRENCEKAVTALEGVQRVENQLTISQFYRYGP"
     gene            complement(2007..2657)
                     /locus_tag="VEL68_14210"
     CDS             complement(2007..2657)
                     /locus_tag="VEL68_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXZ37170.1"
                     /translation="MIQIELTRENVEMLREILASHLSELRMEIACTDRRDFREYLRKR
                     GEFLEGFLQDLERELASAGKGLIHIDRLRKVEIFQGLTDWELKIVAQFFQEERAKAGV
                     SLCKEGEIADRLFILEEGAVNLMTKKGESYELRTPGKIIGWSFLVSPNLYTATVNTLT
                     TCSLLVMKSPDFYYLIHKEPKMGVKVMDNLARVVASRLKGAEEARGEEGAPNRKIA"
     gene            complement(2884..4302)
                     /gene="coaE"
                     /locus_tag="VEL68_14215"
     CDS             complement(2884..4302)
                     /gene="coaE"
                     /locus_tag="VEL68_14215"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949589.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXZ37171.1"
                     /translation="MPTSCDWGKLVRRMELLLRLKSFPVAFKLLKKKEMLSGIPYLRR
                     MSHKSTLCQLISIVRNFDWTVGADLGDFLSPMCPSIIGLSDLPEVYKDGTFRSVVWTK
                     TREDGRKYEASIPRLPAGEYEAVAMAPLVYNPFDPDIILLYANPAQMMLLINALQFED
                     YEVMEFSCVGESSCSDVIAKCYLQGKPSLSIPCYGERRYGHAQDDELAMALPAAILEK
                     ALRGLEALYKRGIRYPISYAGAEMDVARAFPMAYGGMKQLEELRGNDNRILLGVTGGI
                     ASGKSTVARMLEELGAPIIDFDLLSRVVVEPEKPAWQEIVSYFGEQVLLPDKTLDRKK
                     LSEIVFQDPEKRKKLEGFTHPRIHQEFVRLVKEYTARNPEVIIQAVIPLLIEAGLQYL
                     FHKLLLVYIPPEMQIQRLMERDKTSREVAQSILGAQLPIDEKRAYADYIIDNSGSLEE
                     TQKQVKEVWQKLKEYQKSKPRT"
     gene            complement(4316..4942)
                     /locus_tag="VEL68_14220"
     CDS             complement(4316..4942)
                     /locus_tag="VEL68_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXZ37172.1"
                     /translation="MRVDLHIHTRPKSPCSAIDPSALLQEARRAGLDGICLTEHQNPW
                     SPGEVEELMKAGGIRVFQGNEITTNQGDILVFGYDRDVKGVVPIQDLRKEVEPVGGLM
                     IAAHPFRGFLLFGISQLQLDLEQACKRSVFQHVDGLEIHNCKVTDPENDMARRVAERL
                     GLLGVAGSDAHRLDEVGKCVTIFEREVRAEQELIEEIRAGRFTVEVRR"
     gene            complement(4950..5411)
                     /locus_tag="VEL68_14225"
     CDS             complement(4950..5411)
                     /locus_tag="VEL68_14225"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HXZ37173.1"
                     /translation="MITFSRAIVVTGEFLREDFLRLRGILEDHIYAMEIQVDVPLPEG
                     IISSIQGFMKRYTTPVCPKAVDVLQKAVGMSLRDPGWISRVHREIGRQGCQHFAEILV
                     ECGRCLDSARMSHDLGDTLKAQPSASPWEIARSWVKTHPEAQGSCLARPKG"
     gene            complement(5456..5944)
                     /locus_tag="VEL68_14230"
     CDS             complement(5456..5944)
                     /locus_tag="VEL68_14230"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HXZ37174.1"
                     /translation="MTLSFIRNKVVEVEPLADGSLSIFWRLADSLTEMEMTMKVRLPD
                     LEITEACARIIRSPHGECASAPDSIQKIVGVRVGPGLRKIAGGLMGGSASCAELTEAV
                     LEAANAVILHFTVPQIQANAKGTEEERKKRYQDMLRFNPRLVRSCIAFADDSPLMQGI
                     NG"
     gene            complement(6197..>6791)
                     /locus_tag="VEL68_14235"
     CDS             complement(6197..>6791)
                     /locus_tag="VEL68_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ37175.1"
                     /translation="SDSVVDLVEKGAVTNARKSILPGKIVAGELIGTEKLFRFGHENP
                     LLEMHGAGVSHNAELIGKIGNFIAINSALEIDLTGQINAESLNGVQISGVGGQFDFVE
                     GAYFSKGGKSITALTSSAAKGKLSRIVANLPAKAVVTTPRYLTDIVATEYGIAHLRGK
                     SNRQRAEALISIAHPGFRDGLWEAFRKEQAKGKKGLE"
BASE COUNT         1432 a         1945 c         1797 g         1617 t
ORIGIN      
        1 tgggaggatg cgcaaccatg gccccgagtg gaaagatgta cacattaaaa ggctccccgc
       61 agacagctca taccggattt tttgatgcta ccattcctcc cgtcttgacg gtgaattcag
      121 gggacacggt gaccttgagc actatgtggc tcatggatgg ccagctgcgt tgcgggatga
      181 ctcttgaaga gctgatcgcc aaacggcaga cctacataga ccggaagatg ggcccccata
      241 cgttgaccgg gcccattttt gtcagtggag ccgagccggg cgatgtgctg gaggtgcgga
      301 tccaaaaatt gatacccgct agttgcgggg tgaactatca tttcccggcg aagatgaata
      361 ttgggggatt gcccgaagat tttcccatcg gacaattcaa gaccctccaa ctggatgttc
      421 agaagatgga gactaccttt gcccctggga ttgtcatccc tcttaaaccc ttcctgggag
      481 tgatgggagt ggcgcctaaa ccaggagaaa aaagacccgc cgcggtgccg gaatattttg
      541 gtgcaaacat ggatctcaag gaactagtgg ctggaactac cctttacctg ccggtcagcg
      601 cgaaaggcgc tcttttctcg acaggcgacg cccatgcggg ccagggggat ggggaggtca
      661 atgtcaccgc gatcgagacg gctatggagg aggcggtcct ccaatttgtg gttcgaaaag
      721 atttaaagct cgagcgtccc atggcggaaa cgccaactca ctggattgcc atggggttcc
      781 ataaggacct gaatgaagcg gtaaaaattg ctctccggga tgccattcaa tttatctcca
      841 agaccaaagg attaacgccg gatgatgcat actctctatc cagtatggca gtggatttgc
      901 gggtaaccca gattgtcgat ggcaataaag gaatccatgc catgattccg aaggcgattt
      961 tcaagaaata aatcgcccgg gtatcccaaa agggaagttc cccctttagg catacctttt
     1021 tccttcccaa ggactacgcg tcaccctgtt tttctgagtt gaatgatgaa acagccctgg
     1081 cctccgaatt ggaagccagg gctgcctgtt tttcccctgg tttccagccc aagaccgaca
     1141 gtagaaaagg cctccttttt aaggtccgta tcggtagaac tggctgatgg tcaattgatt
     1201 ttccaccctt tggacgccct ctagagcggt aaccgctttt tcacaattct cccggcttcg
     1261 ggcagagatg accatacccc ttaacagcac cgtccctttc ttggccccaa cctcgacgtt
     1321 gcggacatcg gttcccagga catccatcag ccgagcttcg actttctgca ccagggctaa
     1381 ttcacccaat ttttcccgag ccagattttc ctttccggtt acatcaggag aaagaaccgc
     1441 gtcggttatg attttggagg ccgtttcaac tgaaaggtcg gctgtattga tgatcaaatc
     1501 atacaccgaa gggtcatccc agttcgcatg aaagaaagtt tgaatgtacc cgaaagagtc
     1561 ctggtcgctc tgccggagga aacgggccgc ctgcttatcg tcgaatcctc cggtctcgac
     1621 catccgtttc acccgttttt caaaaggggc aatgattcga agatggaggg tcccggggac
     1681 ctccttcagc aaaacctgcc cgccccgccc gacgatcacc acccggccct tgctggccag
     1741 atcgtacagg accgcctggg tcgcatggag gaactttctt ctctccagag acaaggagtc
     1801 ccaaaagggt ggcttctttt cgtcgaactt ctccatttcc ggttctggaa atccaaaacc
     1861 cttcaacagc tgctcaagtt tttctttgtc cacgtactca taattcaaaa accgggctgt
     1921 ttcccgggcg atctccgttc ccaagcttcc cacctgccgg gatacagtga tgaccgccat
     1981 gacggcacct cctttcgggt tggtaatcag gcaatcttgc ggttcggagc accttcctcc
     2041 ccgcgggcct cttccgctcc cttcagccga ctggccacca ctcgagccag gttgtccatc
     2101 acctttaccc ccatttttgg ttccttgtgg atgagatagt agaaatcggg gctcttcata
     2161 accagaaggg aacaggtggt aagggtgttc accgtggccg tgtagagatt cggggagacc
     2221 aaaaatgacc agccgatgat tttcccgggg gtccgaagct cgtaggactc ccccttcttg
     2281 gtcatcaggt tcactgctcc ttcttccagt atgaagagcc ggtcggctat ctccccttcc
     2341 ttgcagaggg acactccagc tttcgctcgc tcttcctgga aaaattgagc cacaatcttc
     2401 agctcccagt cggtgaggcc ttgaaaaatc tccactttcc ggagacgatc gatatggatc
     2461 aaccccttcc ccgcggaagc cagctctctc tccaggtcct gaaggaaccc ctccaagaat
     2521 tctccccttt tcctcaggta ttcgcggaag tccctccgat cggtgcaggc aatctccata
     2581 cgcagttcag aaaggtggct cgcgaggatt tctcggagca tctcgacgtt ttcacgagtc
     2641 agctcgatct ggatcatcgc cctcctccct tcttggttga ttggttctcc cctaggtcat
     2701 ccaatgaccc cccaacaaaa aagcccattc cgcattaagg aatgggcttt ttgagtacca
     2761 tgatcctccc ccttatgggt tagaggttat gggccgatat gatttatccg gattttaaat
     2821 tattttatca cattttgaga gggaagagaa gaaaatcggg cccggcttct tcggcctcgt
     2881 aacctaagtc ctcggcttgc ttttctggta ctccttcaac ttctgccaga cttctttcac
     2941 ctgcttttgg gtctcctcca gggaaccgga gttatcgatg atatagtccg cataggccct
     3001 cttttcatcg atgggcaatt gggcaccgag gatgctctga gccacctccc gggaggtctt
     3061 gtctctctcc atcaggcgtt ggatttgcat ttccggcggg atgtagacca gaaggagttt
     3121 gtggaacagg tattgaaggc cggcttcgat caggagagga atcaccgctt gaataatgac
     3181 ctcggggttc cgggcggtgt attcctttac cagccggacg aattcttggt gaatccgggg
     3241 atgagtaaat ccctccagct tttttctttt ttccgggtct tggaagacga tttcggagag
     3301 cttttttcgg tccagggttt tatcgggaag cagaacctgt tccccgaagt aggatacgat
     3361 ctcttgccag gcgggctttt cgggctcgac aacgacccgg ctgaggaggt cgaaatcgat
     3421 gatcggggct cccaactctt ccagcatccg ggccaccgtc gatttcccac tggcgatccc
     3481 cccggtgact cccagcagaa tccggttgtc atttcccctg agttcttcca attgtttcat
     3541 ccctccgtag gccatgggga atgcgcgggc gacgtccatt tcggctcccg catagctgat
     3601 ggggtaccga atcccccttt tgtaaagcgc ctccaatccc cgcagggcct tttctaggat
     3661 ggcggccggg agagccatgg ccagctcgtc gtcctgggca tgcccatagc ggcgctcgcc
     3721 gtagcagggg atggagagtg acggtttccc ctggagataa cacttggcaa tgacatccga
     3781 gcaggacgac tccccgacgc aggaaaattc catgacctca tagtcttcaa attgcagagc
     3841 attgataaga agcatcatct gggccggatt cgcgtaaagg aggatgatgt cgggatcgaa
     3901 aggattatag accagcgggg ccatggctac ggcctcgtat tccccggcgg gcagacgggg
     3961 gatggaggct tcatattttc ggccatcctc acgggtttta gtccacacta ccgagcgaaa
     4021 cgttccatcc ttgtagacct ccggcaggtc ggaaagtccg atgatcgagg gacacatggg
     4081 ggaaaggaag tcccctaggt cggcgcccac ggtccagtcg aaattgcgga caatggaaat
     4141 gagctgacag agggtgctct tgtgactcat cctcctaagg taaggaatcc cggagagcat
     4201 ctcctttttc ttgagcagtt tgaaggccac aggaaaggat ttcagccgaa ggagcaattc
     4261 catccgtcgg accagctttc cccagtcaca acttgtcggc attcttcctc cgcctctacc
     4321 gccggacttc gacagtgaac cgcccagccc gaatctcttc gatgagttct tgttccgccc
     4381 ggacttcccg ctcaaagatg gttacgcact tccccacctc atccagccgg tgggcgtcgc
     4441 ttccggccac tcccaagagc cccaggcgtt cggcgacccg gcgggccatg tcgttctccg
     4501 ggtcagtcac cttgcagttg tgaatctcca ggccgtccac gtgttggaat acggaacgct
     4561 tacaggcctg ctccaggtcc agttgaagct ggctaattcc gaagaggaga aacccgcgga
     4621 agggatgggc ggcgatcatc aaccctccga ccggctcgac ctctttgcgc aggtcctgga
     4681 tcggcacgac cccctttacg tcgcggtcat aaccaaaaac cagaatgtcc ccttggttag
     4741 tggtgatctc gttcccctgg aagacccgga ttccaccggc cttcatcaat tcttctactt
     4801 ctccgggaga ccacgggttt tgatgttcgg tgaggcagat cccatccaat ccggctcgac
     4861 gggcttcttg caggagcgcc gaaggatcga tggcgctgca gggggactta ggacgggtat
     4921 gaatatgcag atcaacacgc atggcaggtt taccctttag ggcgggccag gcaggaacct
     4981 tgagcctcgg gatgggtctt cacccaggaa cgggcgatct cccaggggga ggcggatggc
     5041 tgagccttca gggtatctcc caaatcatga gacatcctgg cggagtccaa acaccggccg
     5101 cactccacga ggatttcggc aaaatgctgg cacccttgcc gtccgatttc ccgatggact
     5161 ctcgaaatcc aacccgggtc ccggagggac attcccacgg ccttctggag cacatccacc
     5221 gccttgggac ataccggtgt ggtgtagcgc ttcataaacc cctggatgga ggagataatc
     5281 ccttccggca gcgggacatc cacctgaatt tccatggcgt agatgtgatc ttccaggatc
     5341 ccccgtaaac gaaggaaatc ctcccttaag aattcaccgg tcacaactat ggcgcgactg
     5401 aatgtaatca tgaaccgaat ctccagggac ccttttcgcc ctttcccctt ttcacctatc
     5461 cgtttattcc ttgcatcaac gggctgtcgt cggcgaaggc gatgcagctg cgcaccagcc
     5521 gcggattgaa tcgcagcatg tcctggtaac gcttcttcct ttcttcctcc gtccctttcg
     5581 cattggcctg aatctgggga acggtgaagt ggaggatgac cgcgttggcc gcctccagga
     5641 ctgcctccgt cagctcggcg caagaggccg agcctcccat gaggccgccg gcgatcttcc
     5701 gcaggccggg tccgactctt acacccacga ttttttgaat ggagtcaggg gccgatgcgc
     5761 actctccgtg aggggagcgg atgattcggg cgcaggcctc ggtgatttcc aggtcgggga
     5821 gccggacctt catcgtcatt tccatctcgg tcaaactgtc cgccagacgc caaaaaatgg
     5881 agagggagcc atcggccaac ggttcgacct cgactacttt gttccgtatg aaagaaaggg
     5941 tcatgcgtcc tccggagatg cctaaagttt agaaatactc tgtcaaaaat ccgtgtccat
     6001 ctgtgtttac ccattacatc cgtcacatcc gtcattccgg gcttgacccg gaatccagga
     6061 tttctttctg gattcccgtt ttcgcgggaa tgacgaacct actccattaa aattttatgc
     6121 gacgtttagg tttattcgca tccaataatt tctcttgttt ctccgaatcc cgtaggagcg
     6181 ggtttcaaac ccgtccctac tccaatcctt tcttaccctt tgcctgttcc tttcggaagg
     6241 cttcccataa tccgtcccga aaacccggat gggcaatgga aatcaaggct tcggcccgct
     6301 ggcggttgga ttttccgcgc aggtgggcga ttccgtattc ggtcgcaacg atgtcggtca
     6361 ggtagcgggg agtggtgacg acggccttgg ccggcaggtt ggcaacgata cgggaaagct
     6421 tgcctttggc ggcggatgag gtcaaggcgg tgatggattt tcctcctttg gagaagtagg
     6481 ccccctcaac gaaatcaaat tgccccccca caccgctgat ctggaccccg ttcagagact
     6541 cggcgttgat ctgtccggta aggtcaatct ccagggcgga gttgatggcg ataaagttgc
     6601 cgatctttcc gatcagctcg gcgttgtggc tcaccccagc cccatgcatc tccaggaggg
     6661 ggttttcgtg gccgaagcgg aagagctttt ccgtcccgat cagctccccg gccacgattt
     6721 tgcccggaag gatgctcttg cgggcgttgg tgaccgctcc tttctctacc aggtccacca
     6781 cgctgtcgga g
//
LOCUS       DASPRR010000385         6313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2342248, whole genome shotgun sequence.
ACCESSION   DASPRR010000385 DASPRR010000000
VERSION     DASPRR010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342248"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VEL68_14240"
     CDS             <1..312
                     /locus_tag="VEL68_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37176.1"
                     /translation="KAIGWETSDVILMKFWEGMAVSLSAFLAGILLAYVHVFFASSSL
                     FEPVLKGWTVLYPQFRLVPFIDIPQVVALFFLTVVPYTVATIIPSWRAATVDPDSVMR
                     A"
     gene            874..1554
                     /locus_tag="VEL68_14245"
     CDS             874..1554
                     /locus_tag="VEL68_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37177.1"
                     /translation="MIELDEVRKTFNLGRPNEFVALQGISLSVEASKMTVFKGPSGSG
                     KTTLLSLIGCMSRPTSGRIHLFDREITSLPERFLTEIRRKTFGFIFQQYNLIQGVTAL
                     ENVMAPAYPSGEKFSVLKKRARGLLDLFNISSKADSRVERLSGGEMQRVAIARALINN
                     PQVVIADEPTAHLDTSLSKEFMEIMGRLKESFKTVLIASHDPVVSESPLVECVWNLRD
                     GRIDGEAG"
     gene            1551..2549
                     /locus_tag="VEL68_14250"
     CDS             1551..2549
                     /locus_tag="VEL68_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37178.1"
                     /translation="MNFHPLVIALYVSSALIGFMVLYAVYFGAQILGKWDLRSGSELQ
                     LALERKTYLISSLLAYGFGAQLLSLFLFAFTADQLHPFFVGAMCAAGTLYVNAYGYPA
                     LILKVINFLLAGLWLLVNYVDNRGYDYPLIRKKYSFLLIIAPLIVLEVILQANYFLRL
                     RPDIITSCCGTLFSIGEKNLVSEIVALPGFPMKLVFYGSTAMALAAGVFFYRQGRGGY
                     FFSLMSAGTFVISILSILSFISLYFYELPTHHCPFCILQGEYGYVGYPLYISLFGAGI
                     TGMGVGVLMPFRRVQSLAGIIPPFQKRLTLVSLAFLIIFMGIVTYRMVSTDFVLGG"
     gene            complement(2712..3407)
                     /locus_tag="VEL68_14255"
     CDS             complement(2712..3407)
                     /locus_tag="VEL68_14255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXZ37179.1"
                     /translation="MNYIPATILSMGFFLTSALCFAGEKPELKDQKDKESYSLGYMFG
                     QSLKAQGLDINLEVYNAGIRDAVGGVNPPLSQEEIQKTVVEIQKRVMAARQKEIKEMA
                     EKNLLESGAFLEENKKKEGVKALPSGLQYKVLAEGTGSKTPAATSQVTVNYRGALLNG
                     VEFDSSYKRGKPATFQVDKVIRGWGEALQLMKEGSKWQLFIPPELGYGDRGAGPIPPN
                     SALIFEVELISVK"
     gene            complement(3570..>6313)
                     /locus_tag="VEL68_14260"
     CDS             complement(3570..>6313)
                     /locus_tag="VEL68_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXZ37180.1"
                     /translation="VAPVGGFGKQYQVNLDPNRLQAYGISINRVVEAVRSGNYESAGR
                     LLEFGGTEYNVRGRGYLKSIKDLENIVLTASDTGTPIRIKDIGSVVIGPDLRRGVSDL
                     DGKGDAVSGIVVMRHGQNALEVIERVKAKIKEIEPGLPPGVKIVPIYDRSDLILRSID
                     NLKSTVIEVLITVAFVVFLFLWHIPSALIPVITLPVVILLSFIPFRTLGVTANIMSLG
                     GIAIAMGAMVDAAIVVVEQTHKNLEVWDRTERREDYRSVVIRAVKQVAGPSFFALLVI
                     AVSFLPILVLEGEEGRMFKPLAYTKNLAMIIAAIMAITLDPALRLLFTHMKNFSFRPS
                     WLCRASNAAFVGRIQSEDKHPISRFLIRIYDPIALWSLRWKWVVIGLAVIAMTATVPI
                     YSKLGSEFMPPLEEGSLLYMPTTMPGISITEAQKVLQVTDRIIKGFPEVRYVLGKAGR
                     AETSTDPAPLSMLETVVILKPKPEWRKKDTWYSSWAPEFLKSVFRHITPDHISQEQLV
                     GEMNDALKIPGLANAWTMPIKGRIDMLSTGMRTPVGLKVSGGDLDTIEEIGARIESIL
                     PSVKGTRSVFAERTGSGYFLDFEWKREELARYNLSVEEAQAVVEKAIGGDNVTTTVEG
                     RERYPVNVRYMRDFRSDYGALGRVLVPASGGQKQIPLSQLAAIKTVSGPAMIRNEGGL
                     LTGYVYVDIADRDPNGYIEEAGKLIREKVKLPPGYAVSWSGQYEGMERAKERLTVVLP
                     LTIFLILFLLYLNTRSMVKTLMILLAVPFSAIGAFWFLYALGYNLSIGVWVGLIALLG
                     VDAETGVFMLLYLDLAYEQAKREGRLKSLSDLQEAIRYGAVKRLRPKFMTVATMFLGL
                     IPILWSTGTGADVMKRIAAPLIGGIFTSFLLELLIYPAVYQVWKWNFDLKKTLRGRG"
BASE COUNT         1301 a         1814 c         1692 g         1506 t
ORIGIN      
        1 aaggccatcg ggtgggagac ttcggatgta atcctcatga agttctggga ggggatggcg
       61 gtttccctct ccgcctttct cgccgggatc ctcctggctt atgtccatgt ttttttcgcc
      121 tcctcgagcc tttttgaacc cgtgttgaaa ggctggacgg tcctgtaccc gcagttcagg
      181 ctcgttccct ttatcgatat tccccaggtg gtggcacttt tcttcctcac cgtcgtcccc
      241 tacacggtcg cgacgatcat cccctcctgg cgcgccgcca ccgtggaccc ggattcggtg
      301 atgagagcgt agtttttttt gtagggcccg ttccccgatc ggaccgacca ttatatggaa
      361 aacaaccttc aatcgcgaaa acctcttcgc ctgaaaggct ttgattacgg gagcaaatcc
      421 cacgtctatt tcgtcaccct atgcacggcg gaacggcacc ctctttttct agggagcaag
      481 attgctaaaa ccctggaaga ggaaatggaa ttcaggaggg caaagaagga aatccgtttg
      541 ttttgctatt gtattatgcc ggatcattta catttattgt tttctctggc cgacgattat
      601 ccgaaaagaa tgcaggattg ggtttcggct ttcaaacgtc atacggcgaa aagaatcttc
      661 gatttatatg agcttcggcc gttgtggcag aagaattttt ttgaccggat tgtgagacaa
      721 gaagaatcac tcctgaatat ggcaaaatac attgtccata accctgttcg gaaagggatc
      781 gtatcgagct gggagtcata tccttatagc aaaatgattg atcctttgcc cctttagggc
      841 aatcggcccg atcggggatc gggccctaca gaaatgattg aattagacga agtgcgaaaa
      901 accttcaacc tcggccggcc caacgaattc gtggccctgc aggggatcag cctgtccgtt
      961 gaggccagca agatgaccgt cttcaaaggc cccagcggct ccgggaagac gaccctcctg
     1021 agcctgatcg ggtgcatgtc ccggcccacg tccgggcgga ttcatctttt tgaccgggaa
     1081 atcaccagcc tgccggaaag gtttctcacc gaaatccggc ggaagacctt cgggttcatc
     1141 ttccaacagt ataatctgat ccagggagtc accgccctgg agaatgtgat ggcgccggct
     1201 tacccttcgg gggagaaatt ctccgtcttg aaaaaaagag ccaggggcct tctggatctt
     1261 ttcaacatct cttccaaggc cgactcccgg gtggaaaggc tttccggcgg ggagatgcag
     1321 cgggtggcca tcgctcgggc cctgatcaac aatccccagg tggtcatcgc cgacgagccc
     1381 accgcccacc tcgacacctc tctatccaaa gagttcatgg aaatcatggg ccgcctgaaa
     1441 gagagcttca agactgtcct gatcgccagc cacgacccgg tcgtgtccga atcgcccctg
     1501 gttgagtgcg tgtggaacct gcgggacggc aggattgatg gtgaagccgg atgaactttc
     1561 atcccctggt catagccctt tacgtctcct ccgcgctcat cggcttcatg gtcctctatg
     1621 ccgtttattt cggcgcgcaa atcctgggca agtgggacct gaggagcgga agcgagctgc
     1681 aactcgccct ggagaggaaa acctacctca tctccagcct tttggcctac ggtttcggcg
     1741 cccagctgct atccctgttt ctattcgcct tcaccgccga tcaactgcac cctttcttcg
     1801 tgggagccat gtgcgcggcc gggacgctct acgtgaacgc ctacggctat cccgccctca
     1861 tcctgaaggt cattaatttt ctgctggccg gtctctggct gctggtgaat tacgtggaca
     1921 accggggata tgactatccc cttatccgca agaaatattc ctttctctta atcatcgctc
     1981 ccctgattgt cctggaagtg atcctgcagg ccaactattt cctgcggctc cgccccgata
     2041 tcatcacttc ctgctgcggg accctgttca gcatcgggga aaaaaacctg gtgtcggaaa
     2101 ttgtcgccct ccccggcttc ccaatgaagc tcgtttttta tgggagtacc gccatggctc
     2161 tcgcggccgg ggttttcttt taccggcagg gaaggggagg ctacttcttt tccctgatga
     2221 gcgcgggaac tttcgtgatc tcgatcctgt ccatcctatc tttcatctcc ctctatttct
     2281 atgagctccc cacccatcac tgtcccttct gcatcctcca gggagaatac gggtatgtag
     2341 gataccctct ctacatctcc cttttcgggg cagggattac cggcatgggg gtcggtgtgc
     2401 tcatgccctt ccgtagggtc cagagtctag ccgggatcat tcctcccttc cagaaaaggt
     2461 taactctcgt ttctctggct ttcttgataa tctttatggg gatcgtgact taccggatgg
     2521 tctctacgga ttttgttctc ggtgggtaga atttctgaat tcgcagatta tgcagatttc
     2581 gcagattcat gacagattat ttccaaatct tattaccggc gaggcgtttc tcttttattg
     2641 tttccagggc tttaccctcg gcgcaatctg cgtaatctgg ggatatctat tagcctgtgg
     2701 ccaaccaggg attatttgac ggatatcaat tcgacctcga agatgagggc gctgtttgga
     2761 ggaatggggc cggccccacg atcgccatag cccaattcag gcggaatgaa aagctgccac
     2821 ttcgagcctt ccttcatcaa ctgcagagct tccccccatc cgcgaatgac tttatcgact
     2881 tgaaaggtag ccggcttgcc tcttttgtag gagctgtcga actcaacacc gtttaggagg
     2941 gctcccctgt agttgaccgt gacttgactg gttgctgctg gcgtcttgct gccggttcct
     3001 tccgccagga ctttatactg caggccgctg ggaagagctt ttactccctc tttcttcttg
     3061 ttctcttcga ggaaagcccc gctttccaag aggttttttt cagccatctc ttttatttcc
     3121 ttctgacggg cggccatgac ccgtttctgg atttccacaa cggtcttttg gatttcttcc
     3181 tggctcaacg ggggattcac cccacccacg gcatcccgga taccggcgtt gtaaacctcc
     3241 aggttgatat ccaatccctg agctttcagg ctctgtccga acatgtaccc caggctgtag
     3301 ctttccttat ccttctgatc tttcagctcg ggtttttccc cggcgaaaca aagagcggaa
     3361 gtcaaaaaga agcccatgct gagaattgtt gcaggaatat aattcatttg aatctccctc
     3421 tttctggacc ggacggcatt cacgtattgt tttcatccgc agattacgca gattttgcag
     3481 agcaacgaaa ggttattccc atttcttaat gccagcaaac catttccgcc ttctcctata
     3541 cgttctgtga aatctgcgga ttccaccaat catccgcgtc cccttaatgt ctttttcaaa
     3601 tcaaaattcc atttccagac ctggtaaacg gccgggtaga tcagaagctc caataagaag
     3661 gaggtaaaga ttccgccaat caatggcgca gcgatgcgtt tcatcacgtc cgcaccggtg
     3721 ccggttgacc aaaggatggg aatgaggccg agaaacatgg tggccacagt catgaacttg
     3781 ggccggagtc tcttgaccgc cccgtagcgg atggcttcct gcaggtcgct caggctcttc
     3841 aagcgcccct ccctcttggc ctgctcatac gccaggtcca ggtagaggag catgaaaacc
     3901 cccgtctccg cgtccacccc cagcagggcg atcaggccta cccagacccc gatgctcagg
     3961 ttgtagccga gagcgtagag aaaccagaag gccccgatgg cggagaaagg cacggccagg
     4021 agaatcatga gggttttgac catggagcgg gtattcaggt agagaagaaa taggatgagg
     4081 aagatggtca ggggcaatac gacagtgagc cgctccttgg cccgctccat gccctcgtac
     4141 tgcccgctcc aggaaactgc atagcccggc ggaagcttca ccttttcccg gatcagcttt
     4201 cccgcttctt cgatgtagcc attggggtcg cggtcggcaa tgtccacata cacgtagccg
     4261 gtcaataggc cgccttcatt acggatcatg gccgggccgc tgaccgtttt gatggcggcg
     4321 agctgggaaa ggggaatctg cttctgccct cccgaggcag gcaccagaac ccttcccaag
     4381 gcgccgtagt ccgagcggaa gtcgcgcatg tagcggacgt tcaccgggta ccgttcccgc
     4441 ccttccaccg tggtggtcac attatctccg ccgatggcct tctccaccac cgcctgggcc
     4501 tcttccacgc ttaaattgta acgggccagc tcctcccgtt tccactcgaa atccagaaag
     4561 tagccgctgc cggtgcgttc ggcgaagacg ctgcgggtcc ctttcacaga agggagaatt
     4621 gattcaatcc tagcgccgat ctcctcgatg gtgtccaaat ccccgcccga gaccttgaga
     4681 cccaccgggg tccgcatccc cgtgctcagc atgtcaatcc gccccttgat gggcatggtc
     4741 caggcgttgg ccagcccggg gattttgagg gcatcgttca tctccccgac taactgttcc
     4801 tgggagatat gatcgggggt tatatgccgg aagaccgatt taagaaattc gggggcccag
     4861 gaagaatacc aggtatcctt cttccgccac tcgggcttgg gcttgaggat gaccaccgtc
     4921 tccagcatgg ataggggtgc cggatcagtc gaggtctcgg cccggccagc cttgcccaat
     4981 acgtacctca cctccgggaa tcccttgatg atccggtccg tcacctggag gactttctgt
     5041 gcttcggtga tcgagatgcc gggcatagtg gtgggcatgt agagaaggga cccctcctcc
     5101 agggggggca tgaactccga tcccagcttg gaatagatgg gaaccgtggc agtcatggca
     5161 atgacggcca ggccgatcac cacccacttc cagcgcagcg accagagggc gatgggatcg
     5221 tatattcgaa tcaggaaacg gctgatggga tgcttgtctt ctgattggat cctcccaaca
     5281 aaagcggcat tggacgcgcg gcagagccac gagggtcgga agctgaaatt cttcatgtgg
     5341 gtgaagagca gccgtaaagc aggatccagg gtgatggcca tgatcgctgc gatgatcatg
     5401 gccaggttct tagtgtaagc caggggcttg aacatgcggc cttcctcgcc ttccaggacg
     5461 agaatgggga ggaaggagac ggcgatcacc aggagggcaa agaagctcgg gccggcgacc
     5521 tgctttaccg cgcggatgac caccgacctg tagtcttccc ggcgttccgt ccggtcccaa
     5581 acctccaggt tcttgtgagt ctgctccacc actacgatgg ccgcgtccac catagccccc
     5641 atggcgatgg cgattccccc taatgacatg atattggcgg tgacccccag cgtgcggaac
     5701 gggatgaaag acagcaggat aaccaccggg agggtgatta ccggaatgag cgcgctggga
     5761 atgtgccaga ggaagagaaa gacaacaaaa gccacggtaa tgagaacttc aatgaccgtg
     5821 gatttgaggt tgtcgatgga gcgcaggatc aggtcggagc ggtcgtagat ggggacgatc
     5881 ttcacccccg ggggaaggcc gggctcgatc tccttgatct tggccttgac cctttcaatg
     5941 acctccaggg cgttctggcc atggcgcatt accacgatgc cggataccgc gtctcccttg
     6001 ccgtccaggt cggaaacgcc cctgcgcagg tccggcccga tgaccacact cccgatgtcc
     6061 ttgatgcgga tgggggtgcc cgtatcgctg gccgtaagca cgatattctc aaggtccttg
     6121 atcgatttga ggtaaccccg tccccgcacg ttgtattccg tccccccgaa ctccagaagg
     6181 cggcccgctg attcatagtt cccgctgcgc accgcttcta ccacgcggtt gatggagatg
     6241 ccgtaagcct gaaggcgatt ggggtcgagg ttcacctggt actgctttcc gaacccccca
     6301 acgggcgcga cct
//
LOCUS       DASPRR010000386         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_897618, whole genome shotgun sequence.
ACCESSION   DASPRR010000386 DASPRR010000000
VERSION     DASPRR010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897618"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VEL68_14265"
     CDS             <1..319
                     /locus_tag="VEL68_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXZ37181.1"
                     /translation="VEGLRTLIRRHVEWAQKFAGWVQEDKRFELAVLPPMNLVCLRHV
                     RGDDVNQKIMDRLNQSGKVYLTHTKLKDRLTLRFCVGQTHTEERHVVRAWNLIQQAAE
                     EVK"
     gene            complement(347..1531)
                     /locus_tag="VEL68_14270"
     CDS             complement(347..1531)
                     /locus_tag="VEL68_14270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ37182.1"
                     /translation="MKSRSPWFILLIVLLASLAAPLNQFKVPPVMPLLMEAFGASAAQ
                     AGLLMSLFAITGLFLAIPAGFIFQKLGYRLTGMIAILSLVVGAGWGAFSRGFDIMLAS
                     RIVEGVGMSLMSVVAPAVIALWFSPNTRGKAMGVWAVWVPLGSTIMFLLAPPLAGRWN
                     WPGVWWFGCLYAFWVGLLYLVFLKSKSEEPSKPKEGASPGKMQDHGMAKVLSNRDLWL
                     SSLLFCCFNFVFIAFITWAPTFLHQTRGMSLARAAFLVSLASILTIVACPTSGWVSDR
                     TGTRKWVAVIPMALMAPLYPLSFLVGENCFPALVVLLGFISGFVPTGVFSGAVETVGD
                     ERLSGMAMAVIQIGQNAGMLLGPLVFGWIIESGGGWQTAFWVLAPVSILGAVAGWMVR
                     IR"
     gene            complement(1528..>2307)
                     /locus_tag="VEL68_14275"
     CDS             complement(1528..>2307)
                     /locus_tag="VEL68_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ37183.1"
                     /translation="KNYFRAFREAQGVWDLLEKTQELDPQNFDVYYPMGVLHYHLAQL
                     SGIARWITSLFIASGDRERGLKEFETAYEKGRLMKDLAASSLLSAYSGYEKQPARALP
                     LAKALKEKYPDNYNFSFALINILSDLGRFDEAGAVVREIGNEIKAGRPPYRPELWPRY
                     QQSLGKISLDQGEYDKATEYFKQALKDTAPYNSRVRAWALVRLGMIHDARKERKQAED
                     YYRTALDLEGAEGSAQRAAREYLETPYSPPARKENMQGKKP"
BASE COUNT          534 a          687 c          662 g          424 t
ORIGIN      
        1 ggtggaaggg ctgcgtaccc tcatccggcg gcatgtagag tgggcccaaa aattcgccgg
       61 gtgggttcaa gaggacaagc ggttcgaact ggccgtactc cctcccatga atctggtctg
      121 cttacgccat gtgagagggg acgatgtcaa ccagaagatc atggatcggc tgaaccagag
      181 cggaaaggtc tacttaaccc acaccaaact caaggaccgt ctcactctgc gcttctgtgt
      241 gggccagacc catacggaag agcgtcatgt ggtccgggcc tggaacctga tccagcaagc
      301 cgcagaagag gtcaaataaa cgtagggacg ggttacaaac ccgcccctac ctgattcgga
      361 ccatccagcc ggccacggct cccaggatgc tcaccggagc caacacccag aaggccgtct
      421 gccagcctcc tccggattca atgatccaac cgaacaccag cggtcccagg agcatacccg
      481 cgttctgccc gatctggata accgccatag ccatcccgct gagacgctcg tctcccaccg
      541 tttccacggc cccggaaaag acccccgttg gaacgaaccc gctgatgaag cccaacagga
      601 ccaccaaagc cggaaagcag ttttcaccga ccaagaagct gagcggatag agaggagcca
      661 tgagggccat gggaattacc gcgacccact tgcgggtgcc ggtccggtcc gaaacccatc
      721 ctgacgtggg gcaggcgacg atcgtcagga tgctggcaag gctgaccagg aacgcggccc
      781 gggccaggga cattccccgg gtctgatgaa gaaaagtggg agcccaggtg ataaaggcga
      841 taaagacaaa attgaagcag cagaagagga ggctgctcag ccaaaggtcg cggttggaga
      901 ggacctttgc cataccgtga tcctgcatct tgccgggcga agccccctct tttggtttag
      961 agggctcctc tgacttggat ttgaggaaga ccaaatacaa aagccccacc caaaatgcat
     1021 aaaggcatcc gaaccaccac acccccggcc agttccatcg ccctgccagg ggtggagcca
     1081 agaggaacat gatcgtggac cctaggggaa cccagacagc ccagaccccc atggcttttc
     1141 cccgagtatt cggggaaaac cacagggcga taactgccgg ggcgactacc gacatgaggc
     1201 tcatccccac cccctcgacg atccggctgg ccagcatgat gtcaaatccc cggctgaagg
     1261 ccccccagcc cgcgccgacg accagggaga gaatggcgat cattcccgtt agccggtagc
     1321 ccagcttctg aaagataaat cctgccggga tggcgaggaa aaggccggtg atggcaaaaa
     1381 gagacatgag aagcccggcc tgggcagcgg aagcgccaaa tgcctccatg agcaagggca
     1441 tcaccggcgg caccttgaac tggttcagcg gcgcggccag gctggccaaa aggacgatga
     1501 gcaagataaa ccagggtgac cgcgatttca tggttttttc ccttgcatat tttccttgcg
     1561 cgcggggggt gaatagggcg tttccaggta ttcccgggcg gcccgctgcg ccgatccctc
     1621 ggccccctcc aaatccagag ccgtccggta ataatcctcg gcctgtttcc tttctttgcg
     1681 ggcgtcatgg atcattccca gacggaccag ggcccaggcc cggacccggc tgttgtatgg
     1741 ggccgtgtcc ttgagggcct gcttgaagta ttcagtcgcc ttgtcgtatt ccccctgatc
     1801 gagagagatc ttccccagcg attgttggta acgcggccat agttcaggtc ggtagggagg
     1861 cctcccggct ttgatttcgt ttccgatctc ccggacaaca gcccccgcct cgtcgaatcg
     1921 tcccaggtcc gaaagaatat tgatcaaggc aaaggagaag ttataattgt ccgggtattt
     1981 ttctttcagc gctttggcca ggggaagagc gcgagcaggt tgcttctcgt aaccggagta
     2041 ggctgacaac aggctggatg cggctaagtc cttcatgagg cgaccttttt cataagcggt
     2101 ctcaaactcc ttcagccctc tctcccgatc gccggaggca atgaataagg aagtgatcca
     2161 ccgggcgatc ccgctgagct gggccaggtg atagtgaagg acccccatgg ggtaatagac
     2221 atcaaaattt tgcgggtcca gttcctgggt tttttccaac aagtcccaga ccccttgggc
     2281 ctcccggaag gcgcggaaat aattctt
//
LOCUS       DASPRR010000387         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_237874, whole genome shotgun sequence.
ACCESSION   DASPRR010000387 DASPRR010000000
VERSION     DASPRR010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237874"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VEL68_14280"
     CDS             complement(<1..589)
                     /locus_tag="VEL68_14280"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ37184.1"
                     /translation="MKTKSAILYEYNQPLRVEELDLGKPREKEVLVEMKSAGLCHSDL
                     SIIKGVLRMPPLPCIPGHEGAGIVREVGPGVTRVKPGDHVMMVWVPSCGQCYYCRKQQ
                     PYLCAEKDKTRVGTMLDGTYRLQKGTQNINMMMGVGTFSQFNTVSERSVLPINPQIPF
                     ELAAVVGCSVMTGAGAVLNKAKVEPGSSVAVVGTGG"
     gene            773..1123
                     /locus_tag="VEL68_14285"
     CDS             773..1123
                     /locus_tag="VEL68_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37185.1"
                     /translation="MSNHLPSIDHLEVGEEWVKGNGGRGILRKAIGAVKAYLWGHISG
                     ETKRIPLAFSGLETNLDMKIMCKLSDESKGGAEMCPSFELDGTEALKFLAESSIFFRR
                     EFFLDRAGESFRIG"
     gene            1157..2386
                     /locus_tag="VEL68_14290"
     CDS             1157..2386
                     /locus_tag="VEL68_14290"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ37186.1"
                     /translation="MRKRIVGFLPVVLSFTLFLAIAQAQDTIKIGVILPLTGPNAVIG
                     QEEKRGVDMAIEKINAAGGALKKKVELVIEDNRGDPTVIVSAAEKLITHDKVVALTGG
                     YSSVESLALLAAMRKYEPVTVWQGGGAVKMDQMYGKERWFFMLHPRSPDYQRNITDFL
                     YNIQPRPKSIAIAYEDTSYGVDHSKVAKQYLTEKGFELVIFEPFKSGSLDLSSLLTKI
                     KGAKPEIFYWIGYAGDSILLTKQARELGFAPKMMLDTVGVGFPEFRDSLKRDSEYICG
                     IEVWAPSTKFAASLQYPQFFPKTEDWVAEYTKRYSREPNYWSIISYVSLITLTNAINK
                     AATTEKEKLIVALEATDTMTPMGHMKFFKNQFGAIHQSFHEMVVFQWQNGQKVVLWPK
                     GASGGTLSYPFPSWDKR"
     gene            2542..>3350
                     /locus_tag="VEL68_14295"
     CDS             2542..>3350
                     /locus_tag="VEL68_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37187.1"
                     /translation="MDWAIFAQVIANGILTGGLYSLIAVGLTLIFGVMRVINFMHGET
                     LMIGSYLTYFLFTLYGIDPFLSLPLAIFVLFSLGVGIQRFFINPVIDAPHFNQILLTF
                     GLVLIIQNLALILFTGNYVSMTTSYSAVSLKLGPIRLGLARLLGFVIAIALTVMLSLI
                     LKKTEWGKAVRAVTQDREAAMLMGVNVDRVNILAFGVGSAMGGAAGMVTSMIMYIFPL
                     VGMLFILKAFAIVVLGGFGSIAGAVVGSLILGLTESLVTNYLPNGSGWAEGV"
BASE COUNT          699 a          979 c          884 g          788 t
ORIGIN      
        1 cgccccctgt acccacgacc gcgacgctgc tgccgggttc caccttggcc ttgttcagga
       61 cggccccggc tccggtcatc acgctgcagc ccaccacggc tgccagttca aagggaattt
      121 gggggttgat gggcagaacg ctcctttcac tcacggtgtt aaactggctg aaggtgccca
      181 cccccatcat catgttgata ttctgggtgc ctttttgcag ccggtaggtt ccatccagca
      241 tcgttcccac ccgggttttg tccttctcgg cgcacagata gggttgctgt ttccggcaat
      301 agtaacactg gccgcaggac ggaacccaga ccatcatcac atggtccccc ggtttcaccc
      361 gagtgacccc cggccccacc tcccggacga tccccgcgcc ctcgtggcct ggaatgcagg
      421 ggaggggcgg catgcgtaga actcctttta tgatcgaaag gtcgctgtgg cagaggcccg
      481 ctgatttcat ttcgactagc acttcctttt ctcggggttt gccaaggtcc agctcttcga
      541 ctcgcaaggg ttggttgtac tcatacagaa ttgccgattt tgttttcata atccacctcc
      601 gcgaatgata tttaaccccc cccttctcga aaggacaacc ggtctccaaa atcccctggc
      661 ccctaaagat ttggggaaac ctgtccggta aacaagtgct caccacaccc ttaaatgaat
      721 atcattttaa tttctgggac ctgggtatcc cttaggggct cgtctccaga aaatgtccaa
      781 ccacctccct tccatagacc atttggaggt cggcgaagaa tgggtaaaag gcaatggcgg
      841 cagagggatt ttacgaaaag cgataggggc tgtcaaggca tatctctggg gacatatctc
      901 tggggagaca aagcggattc ccctggcttt ttccggccta gaaacaaacc tagacatgaa
      961 aatcatgtgt aagttgtcgg atgaaagcaa aggcggagct gagatgtgcc cctcctttga
     1021 attggatggg accgaagctc tcaagttttt ggcagagagc tctatatttt ttcgaaggga
     1081 attttttctt gacagagccg gagaatcctt tagaattgga taaaattttt cattttccaa
     1141 ttggaggagg gaagccatga ggaaaagaat cgtcgggttc ctgccagtcg tcctgtcctt
     1201 cactctcttc cttgctattg cccaagccca ggataccatc aagatcggcg tcattctgcc
     1261 gctcaccggc cccaatgcag tcatcgggca ggaggagaag cgcggcgtgg acatggccat
     1321 tgagaagatc aacgccgccg ggggtgccct gaaaaagaag gtggagcttg tcatcgagga
     1381 caacaggggg gaccccacag tcatcgtttc cgcggcggaa aagctcatca cccacgacaa
     1441 agtggtcgcc ttgaccggcg gctactccag tgtggaaagc ctggctctct tggcggccat
     1501 gaggaagtac gagcccgtca ccgtatggca aggcgggggg gcggttaaga tggaccagat
     1561 gtacggcaag gaacgatggt tcttcatgct tcacccccgc tcgccggatt accaacggaa
     1621 catcaccgac tttctgtaca acatccagcc ccgacccaag agcatcgcca tcgcctatga
     1681 ggacacctcc tacggcgtgg accattccaa ggtggctaaa cagtacttga ccgagaaagg
     1741 cttcgagctg gtcatcttcg agccctttaa atccgggtct ttggacctct cgtcgctcct
     1801 caccaagatc aagggggcca agccggaaat attctactgg atcggctatg ctggcgattc
     1861 catcctcctg accaaacagg ccagggagct ggggtttgcc cccaagatga tgctggacac
     1921 ggtgggggtg gggttccccg agtttcggga ttccctcaag agggactccg agtacatctg
     1981 cggcatcgaa gtgtgggccc cctctaccaa gtttgccgct tctctccagt atccgcagtt
     2041 cttccccaag accgaagact gggtggcgga atacaccaag cgctacagcc gggaacccaa
     2101 ttactggtcc atcatttcct acgtgtccct gatcaccctg accaacgcca ttaacaaggc
     2161 cgccacgacg gagaaggaaa aactgatcgt cgccctggaa gccacggaca ccatgacccc
     2221 catgggacat atgaagttct tcaagaacca gttcggggcc atccaccaga gcttccatga
     2281 gatggtagtg ttccagtggc agaacggcca gaaggtcgtc ctgtggccga aaggggcctc
     2341 cgggggaaca ctctcgtatc ccttcccttc ttgggataaa cgctgaaatg ccttcggagt
     2401 ttcgggtttc gagtttcagg ttgcaggatc tttcaccaac ccttactcgg accctgttat
     2461 taaatttaga cgtttcgctt tccagtcgct ttttttcaac tttaggcact ccgaacttca
     2521 ggcactctag gcactaattc catggattgg gccatctttg ctcaggtcat cgccaacggg
     2581 atcctgaccg gcgggcttta ttctttgatc gccgtaggcc ttaccctcat tttcggggtc
     2641 atgcgggtga tcaatttcat gcatggcgag accctgatga tcgggtccta tctcacctac
     2701 ttcctcttca ccctctacgg catcgatccg tttctctccc ttcccctggc cattttcgtc
     2761 cttttctccc tgggagtggg aattcagcgc ttcttcatca atccggtcat cgacgcccct
     2821 catttcaatc agattctcct caccttcggt ctggtcctga tcatccaaaa cctggccctg
     2881 atccttttca ccggaaatta tgtctccatg accacgtcct attccgccgt ttccctgaaa
     2941 ctcggcccca tccgactggg cctggcccgt ctcctggggt tcgtcatcgc catcgccctc
     3001 accgtcatgc tcagcctgat cctgaaaaaa accgagtggg gaaaggctgt gcgggccgtc
     3061 acccaggacc gggaggcggc catgctcatg ggagtgaacg tggaccgggt gaacatcctg
     3121 gccttcggag tggggtcggc catgggcggg gccgcgggca tggttaccag catgatcatg
     3181 tacattttcc ccctggtggg catgctcttc atcctgaagg cttttgccat cgtggtcttg
     3241 ggggggttcg ggtccatcgc cggagcggtg gtcggaagcc tgatcctggg actgaccgag
     3301 tctctggtta ccaactacct ccccaacggg tccgggtggg ccgaaggggt
//
LOCUS       DASPRR010000388         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_574489, whole genome shotgun sequence.
ACCESSION   DASPRR010000388 DASPRR010000000
VERSION     DASPRR010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574489"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VEL68_14300"
     CDS             <1..217
                     /locus_tag="VEL68_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ37188.1"
                     /translation="GRPVTLAASMAKRYATDMAMKVTVDAVQILGGYGVMQDYRVERY
                     MREAKIGQIVEGTNQIQRIIIARELLK"
     gene            complement(318..1421)
                     /gene="rodA"
                     /locus_tag="VEL68_14305"
     CDS             complement(318..1421)
                     /gene="rodA"
                     /locus_tag="VEL68_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HXZ37189.1"
                     /translation="MLDRRLIINFDWPTLVTAFIISCLGILNIYSSTFPHTGAATPLF
                     IKQTYWLILGLGLAILVLGFDYRTFVRFSYPFYLLCLLLLILVMAFGRTTSGSQRWLP
                     MGFFSFQPSELTKIALILAMTRFFTEKENPRGYGLRELGIPFILVGLPILFIFKQPDL
                     GTVLLLLLIFTSILLFVGVRFKTWAFLGAGVSAAIPLFWIFLKEYQKNRLLTFINPDL
                     DPLKTGYHISQSKIAVGSGTIFGKGFLKGTQSQLHFLPEQHTDFIFSVLGEEWGFAGC
                     IVLLFLLLFLISRGLKIAKTSKDRAGATLAIGVTAMLFWQSFINVGMVVGILPVVGVP
                     LPLVSYGGTAMVTNLIGIALLMNVSMRRFMLSP"
     gene            complement(1425..3302)
                     /gene="mrdA"
                     /locus_tag="VEL68_14310"
     CDS             complement(1425..3302)
                     /gene="mrdA"
                     /locus_tag="VEL68_14310"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXZ37190.1"
                     /translation="MRLKLDKNETPEISKAFLLANIAIIVAFSLIFSRLWYLQVLKGG
                     YYRALSENNRIRIQEIAAPRGTLFDRNGVPLVDSFPSFDVSLYRQDVPDMEALIRSLS
                     RILSTPEEKIRSRLEGARGIPLHQPLKIKTNITREELAMVETRRLDLPGVVVDVVIRR
                     NYPYKNLASHLIGYLGEISQEELVQEEFLNHKLGYLVGKYGIERKFELDLMGENGGRQ
                     IEVNASGQKIRVLGQVEPNPGNNLYLTLDLELQRAAEEAMAGKRGAIVAMNPRNGDIL
                     ALVSKPDFDPNLFARGISAENWKSIVDNPAHPLQNRTIQGQYPPGSVFKILVAMAGLE
                     EKMITPETAFQCTGVYPFGNREYRCWNKEGHGWITLRRAIVESCDVYFYQLGMRLGVN
                     RIAKYASAAGLGDPTGFPLGLEKPGLVPTSSWKMKRFGTPWQAGENLTVAIGQGYNLA
                     TPLQMACTLSALFNGGNYFQPRIVQTILAPHGESLKEFPPLVLRNIPISSETIEFVRE
                     ALWGVVNSPTGTGARARVEGFNVAGKTGTSQVVQRKEGKSESTSLELQDHAWFACFAP
                     AYNPEITVVVLVEHGGGGGAAAAPVARQVLEYYYQKMKNLPSAPARIAAQPTAARSDE
                     G"
     gene            complement(3305..3817)
                     /gene="mreD"
                     /locus_tag="VEL68_14315"
     CDS             complement(3305..3817)
                     /gene="mreD"
                     /locus_tag="VEL68_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXZ37191.1"
                     /translation="MFRFFIFLLTAFLLTLLESTLVSLIFPSYLKPDLMILLVVFLGI
                     SFPLLPGALLVFFCGLLYDTFSGGPLGLFAFVYLCIFFSLKVLAKILILGETLVFRII
                     LVAVLMSLQSVLLMVLPMFLGIASHLARRPSIWVLPQMLITCAAIWPLFYLFRRVDIP
                     PAEESFPAVP"
     gene            complement(3821..4741)
                     /locus_tag="VEL68_14320"
     CDS             complement(3821..4741)
                     /locus_tag="VEL68_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37192.1"
                     /translation="MSLKEIASALENLRKDMRALQVDFSSFKEKREGFSELKGVLRRR
                     GLDLFRQNPTQHLCFPPVFSPEKKEKFYELFKRYSFRLFLREILIRKGVFRVSEVVRF
                     STPETGRKYVHILMELGLAEALGDSQYRLPYAPPASLGPTLEWFMAEVLRREFSCPAL
                     YGLRCRGSRYGGDYDVVALLEGSLLYLEVKSSPPKNIEGDEIHAFLARLKDLIPHLAL
                     FFVDTELRLKDKIVSFFETELFALGLRDNGNPIRIQKIGDEIFFAPPWVYILGSKRSI
                     VKNLGVCFHHHFTHPLSFLAGEGFGELGKG"
BASE COUNT         1266 a         1312 c         1436 g         1033 t
ORIGIN      
        1 ggggcggccg gtgaccctgg ccgcttccat ggccaaacga tatgccacgg atatggccat
       61 gaaagtgacc gtggatgcgg tgcaaatcct cgggggttac ggggtcatgc aggattaccg
      121 ggtggagcgt tatatgcggg aggccaaaat cggccagatc gtggaaggta ccaatcaaat
      181 ccagaggatc atcatcgctc gggaactatt gaaataaaag tttcgagttt cgggttccgt
      241 gtttcgagtt agaaaccccc gagctacggt tctgctttca acttggaacc cgaaacttgg
      301 aacccgaaac tttttggtta agggctaagc atgaaacggc gcatactgac gttcatcagc
      361 agggcgatcc cgatcaggtt ggtcaccatc gctgtgcccc cgtagctcac cagggggagg
      421 ggaaccccta ctaccggaag aatccccacc accattccca cgttgataaa actctgccag
      481 aagagcatgg ccgttacccc gatggccagg gttgctcccg cccgatcctt tgaggttttg
      541 gcgatcttta gcccccggga gatgaggaag agaagaagga agagaaggac aatacaacct
      601 gcgaagcccc attcttcgcc gagcaccgag aagatgaagt ctgtatgctg ctccggcaag
      661 aaatggagct ggctctgggt ccccttaaga aatcccttgc cgaagatggt ccccgaaccc
      721 acggcgatct tagattggct gatgtgatag ccggttttca gaggatccag gtcaggattg
      781 atgaaggtga gcaggcggtt cttctggtat tccttgagga aaatccagaa gagggggatc
      841 gctgccgaaa ctcccgctcc caggaacgcc caggtcttaa accgcacccc gacaaaaaga
      901 agaatggagg taaagatcaa tagaagaaga agcacggtac ccaagtcggg ctgtttgaag
      961 atgaagagga tcggcagccc aaccaggata aaaggaatcc ccagttcgcg gaggccatac
     1021 cccctggggt tttccttctc tgtgaagaag cgggtcatgg ccagaatcag ggcaatcttg
     1081 gtcagctccg aaggctgaaa ggagaaaaag cccatgggaa gccacctctg ggaaccggaa
     1141 gtagtccgcc cgaaggccat gaccaggatg agaagaagaa ggcacagaag gtagaagggg
     1201 taggaaaagc ggacaaaagt acgatagtca aagccgagaa ccaaaatggc cagcccgagc
     1261 cccagaatca accagtaggt ctgtttgata aagagcgggg tggctgcgcc ggtgtgggga
     1321 aaggtggaac tgtagatatt cagaatcccc aggcaagata tgatgaaggc ggtgaccagg
     1381 gtaggccagt caaagttaat gatcagacgc cggtcgagca tgatttatcc ctcatcagac
     1441 ctggcggctg tcggttgagc agcgattcgg gcaggggccg aaggaaggtt tttcattttt
     1501 tggtaatagt attctaaaac ctgccgggcc accggagcgg cggccgcccc gcctcccccg
     1561 ccgtgttcca ccaaaaccac tacagtgatc tccggattgt aggcgggagc gaagcaggca
     1621 aaccaggcat gatcctgcag ctccagagag gtggactcgc tcttcccctc ctttctttga
     1681 acaacttgag aggtccccgt ctttccggcc acgttgaatc cctcgacccg agctctggct
     1741 cctgtaccgg tgggagaatt aaccaccccc cagagggcct cccggacaaa ctcgatggtc
     1801 tcggaggaga tgggaatatt tcttaagacc agggggggaa actccttcaa gctctcccca
     1861 tggggcgcca ggatggtctg gacgatgcgc ggctgaaagt aatttcctcc gttgaagaga
     1921 gccgagaggg tgcaggccat ctgaagggga gtggccaggt tataaccctg gccgatggcg
     1981 accgttagat tttccccggc ctgccaagga gttccgaacc tcttcatttt ccaggaggag
     2041 gtgggaacca aacctggttt ttccaggccc agggggaaac ccgtggggtc tcccaggcct
     2101 gccgcggagg cgtatttggc aatccgattc actcccaacc gcatgcccag ctggtagaaa
     2161 tacacgtcgc aggattccac gatggctcta cgcagggtga tccatccatg cccttcctta
     2221 ttccaacatc gatattccct atttccgaaa ggatatactc cagtgcactg gaaagcggtt
     2281 tccggggtaa tcattttctc ttccagaccg gccatggcca ccaggatttt gaagaccgat
     2341 ccagggggat actggccctg gatggtccgg ttctgcaagg gatgggccgg gttatccacg
     2401 atactcttcc agttttcggc ggagattccc ctggcaaaga gattggggtc aaagtccggt
     2461 ttgctgacca aggccagaat gtcgccgttg cggggattca tggccacgat ggctcctcgc
     2521 tttccggcca tggcttcttc ggcagccctc tggagctcca agtccagggt gagataaagg
     2581 ttatttcccg gattgggttc aacctgtccc agaacccgga ttttctgtcc actggcattc
     2641 acttcgatct ggcggccccc gttttcgccc atcaggtcca gctcgaattt cctttcgatc
     2701 ccgtatttcc ccacaaggta ccccagcttg tgattcaaga attcttcctg cacaagttct
     2761 tcctggctaa tttcgcccaa atagccgatc agatgggagg ccagattctt atagggatag
     2821 ttccggcgga tgaccacgtc tacgactacg cccggcaggt caaggcgccg agtctccacc
     2881 atagccagct cttcccgagt gatgttggtc ttgatcttca gcggctggtg gagcggaatg
     2941 ccccgggctc cctccagtct ggaccttatc ttttcctcgg gtgtggagag gatacggctg
     3001 agggaccgaa tcagggcttc catgtccgga acgtcctgcc ggtagagaga tacatcgaag
     3061 gatgggaaac tgtccaccag aggcacccca tttcgatcga aaagggttcc ccggggcgcg
     3121 gcgatctctt gaattcggat gcggttgttc tccgagagag ctcggtaata tcctcccttc
     3181 aggacctgga gataccagag gcgggagaag atgagagaga aggctacgat gatggcgatg
     3241 ttggccagaa ggaaagcctt gctgatctcg ggggtttcgt ttttatccag tttaagccgc
     3301 atcgctacgg aaccgcggga aaggattctt cggccggtgg gatatccact cggcggaaca
     3361 gatagaaaag cggccagatg gctgcgcaag tgatcaacat ttggggcaga acccagatcg
     3421 atgggcgccg ggcgagatga gaggcgatcc ccagaaacat ggggagaacc atcagaagga
     3481 cggattgaag gctcatcaac accgccacca gaatgatccg gaagaccagg gtttctccca
     3541 aaatcaggat tttcgccaga accttcaggg aaaagaaaat gcagaggtac acaaaggcaa
     3601 agaggcccaa aggcccgccc gagaaggtgt cgtagagaag accgcagaaa aaaaccagaa
     3661 gggccccggg aaggaggggg aaggagattc ccagaaagac gaccaaaagg atcatgaggt
     3721 ccggcttcag ataggaagga aaaatcaagc tgaccagggt ggattcaagc agggtcagaa
     3781 gaaaagcggt cagaagaaag atgaaaaagc ggaacaagga tcagcctttc ccaagttcac
     3841 caaacccctc tccagccaaa aaagaaaggg gatgggtgaa gtgatggtgg aagcagacgc
     3901 ccaggttctt tacgatggac ctcttgcttc ccagaatgta gacccatgga ggagcgaaga
     3961 aaatttcgtc gccgatcttc tgaatccgga tcgggttccc gttgtcccgg agtcccaggg
     4021 cgaaaagctc ggtctcgaaa aaggaaacga tcttgtcttt cagccggagt tccgtatcca
     4081 caaagaagag cgccaaatgt gggatgaggt ctttcaaacg ggccagaaag gcatggattt
     4141 cgtctccctc gatgtttttc gggggggagg atttgacttc caggtaaagg agcgaaccct
     4201 ccagcagggc cactacgtcg tagtcccctc cgtaccggct accccggcag cgcaagccat
     4261 aaagggccgg gcaggaaaat tctcgccgca gaacctcggc catgaaccac tccagagtgg
     4321 gaccgagact cgccggagga gcgtagggca aacggtattg ggaatccccc agggcctcag
     4381 ccagtcccaa ttccatcaaa atatgaacgt actttcggcc cgtttccggg gtggagaagc
     4441 ggaccacttc ggagactcgg aaaacccctt tgcggatcaa aatctcccgg aggaagaggc
     4501 gaaaggagta ccttttaaag agctcgtaga atttctcctt tttttcggga gaaaaaaccg
     4561 gcggaaagca gaggtgctga gtcggatttt gccggaaaag gtccagaccc ctgcggcgca
     4621 ataccccctt aagttcagaa aacccctccc gcttttcctt gaaggaagaa aaatctacct
     4681 gcaaggctcg catgtctttc cgcagatttt ccagagcgct ggcgatctct ttcagggaca
     4741 tgggaaaatg gcgccataga ttcgtgtcag ggtcagtctt ggttctagtc ttagtaaaaa
     4801 ccgtatactg accctaatca cctacactga caatattctt ttttaatata cactgattcc
     4861 ccggggaaaa caatgggtta cttgagcttg cccaccttca cttttgcgag tctcttttct
     4921 gctaaaaagc aagcttgtgg cgcgcaacgc cctcgctttc gggccgggca atgcctgaaa
     4981 ttcaatcgat catacgccta cttgccaaaa agtgaaggtg ggcaaggtta cttgagtttg
     5041 cccttcc
//
LOCUS       DASPRR010000389         7845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_94404, whole genome shotgun sequence.
ACCESSION   DASPRR010000389 DASPRR010000000
VERSION     DASPRR010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94404"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VEL68_14325"
     CDS             complement(<1..320)
                     /locus_tag="VEL68_14325"
                     /inference="COORDINATES: protein motif:HMM:NF014101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HXZ37193.1"
                     /translation="MAGGLTEEGSGRFAQTKSGEISLTGYEHMLIISLMARPMKCRCV
                     RCSPNSYYFKPRGIPLMALEEVSLRMDELEAIRLADYQGLYHEEAAAKMEISRATFGR
                     VLNEA"
     gene            259..606
                     /locus_tag="VEL68_14330"
     CDS             259..606
                     /locus_tag="VEL68_14330"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXZ37194.1"
                     /translation="MSPLLVCANRPEPSSVNPPAIAVMMELGIDISGHRSKSPVEFMN
                     EKFDFVIALCDHVAESCPVLSGGTQRIHMGFPNPAAASGSDKERLMAFRKVRNQIREE
                     VVAFLTKRGAQGH"
     gene            673..873
                     /locus_tag="VEL68_14335"
     CDS             673..873
                     /locus_tag="VEL68_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37195.1"
                     /translation="MKIAKKKEVMDLKPLFFKGTRLYGLVAVFLVCTFFLPPDPIARA
                     GETEVLILHTNNVAGYLFPCPT"
     gene            883..1872
                     /locus_tag="VEL68_14340"
     CDS             883..1872
                     /locus_tag="VEL68_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37196.1"
                     /translation="MLGGLARRVAYVEKIQRGNQRALVVDSGDLFFNFQSYSDSEKAM
                     KKAQIIGRAYRHMGAAAVNVGCLDLLQGVDFLRQEFSQGLPLVSANLLDPASKGPIFP
                     PYVLKEIGELRIAFFGLLSPESGPEISPVIRQANEGKILIGDPVEAAKETLRKLQGKA
                     DLVILLSDLGLYKDQMVALAVPGIHFILGGHEGRFTRKAVQAGMTHIFQSSHKGMYVG
                     QLQLVLENPSKPFKDDGQVQYLQERIDGLDLNIRALEGAKKRQPGGDTSNFDRSIQEI
                     TRQRNALQEELKRAKEVGIQGNRFLFRLEALEKILPENEEVKKWISGAGVDKD"
     gene            2059..2940
                     /locus_tag="VEL68_14345"
     CDS             2059..2940
                     /locus_tag="VEL68_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXZ37197.1"
                     /translation="MKKNLKWVIPVVAVVGIIVFGYLKFFAWNPERQVLAMINDRKIT
                     VAQFDREIAKIPTPFQDVFREEPRQYLEQWITKEIVLQEARRLGVQPDPAAKGDDVDL
                     SIMQNLLKKEVLDKIQVDPKEVAEVYKQHKDQMGKKSLSEVTPMLEEAIREVKGKEKV
                     EEYVASLKNKAKIEINESRLRDVTALPPPTNTKEDFQQAMKNGKPALVDFGANSCMPC
                     RQIRPILKEIEKEYTGKASILIIDVYKYQDLAREYRVQLIPTLVFFNKEGKESFRHVG
                     AWDKDSIVGKLKEAGAA"
     gene            3303..4526
                     /locus_tag="VEL68_14350"
     CDS             3303..4526
                     /locus_tag="VEL68_14350"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HXZ37198.1"
                     /translation="MHRGSIGLKSVLVLSILIIYSIAGAALALGQSDKDKDALPRPER
                     GIAIYTEFSGVIVPPGESVRMELTADNKGKRDENISMKISEVPKGWRASLKAPNFSVD
                     AVPVPATKTRTMTFLAEPDKTVKPGNYLFQIDAQTEDGKFTSVQKINVTVREKTPGQE
                     DFTVTTSYPVLQGQTDAKFEFSLEVNNKSEVDKSFNLSAQAPAKWETNFKPAYEQKQI
                     SSFRIKGGSSQTVTVEVTPPRDAQSGSFPLLVSISSGDKKQDVKLTVVLTGIYKLDAG
                     TPTGLLSLEALAGKPANMSVFVKNTGSAVNKNISFSSFKPENWKVEFKPEKIEALEPN
                     ALKQVEVIITPAGQALVGDYSVGLSVDGEKGSSKTVELRVSVKTSAAWGWVGIIIIVL
                     VIAGLGGLFLWLGRR"
     gene            4528..5286
                     /locus_tag="VEL68_14355"
     CDS             4528..5286
                     /locus_tag="VEL68_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37199.1"
                     /translation="MNGQTVIETQDLTKVYGEQTAVNRLTLKINQGEVFGFLGPNGAG
                     KTTTLLMLLGLSEPTSGKAWVCGFDPTREALKVKRVVGYLPENVGFYDDINAVQNLLF
                     ISRLNGIPDHVALAKIEEMLNLVDLKPEPKKKVGEFSRGMRQRLGIAEVLLKNPQVVF
                     LDEPTLGLDPDGSLQMLNLIQSLSRDKKLTVFFSSHLLDQVQKISTRIGIMLKGSMVA
                     VGRIEDLAKEKLGLEEGKYTLEELYMKYFKEGQP"
     gene            5292..6248
                     /locus_tag="VEL68_14360"
     CDS             5292..6248
                     /locus_tag="VEL68_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37200.1"
                     /translation="MITICRKELADHFSSTRFTLLFYLILMVSMVIAFMVGTGLKEEL
                     KDVAKPTFVFLMLFTSPGKFLSLIQFISIFGPIIGIVLGFDAVNRERSARTLSKLASQ
                     PIFRDYIINGKFLAGLVTIVIMQLAIVLMISGLGLLLIGVVPGPEEIYRLIIYLVISI
                     FYIGFWLGISILFSVSFRSMATSALASIACWIFFFFFVPMGAGVLADVIAPFDPAQAS
                     QTPELVIKNEQTRKVISLVSPMTLYSEATTIILDPLRKTTQSLILMGPFERLSISRFQ
                     NPLPLSESVYIVLPHLITLIAITLVCFAISYAVFMRQEIRST"
     gene            complement(6343..6735)
                     /locus_tag="VEL68_14365"
     CDS             complement(6343..6735)
                     /locus_tag="VEL68_14365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXZ37201.1"
                     /translation="MKMEVNLEKDFLFRAKCRGHEVVTDQQQKEGGADTAMTPAELFV
                     ASLGNCIGVFAVRFCKRHNLPTEGMKVLLDWTYAQDPARFGSIKAELHYPHDIPEAEK
                     KGLLRMAETCFVHETILHKPEIKVELKP"
     gene            complement(7037..7540)
                     /locus_tag="VEL68_14370"
     CDS             complement(7037..7540)
                     /locus_tag="VEL68_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXZ37202.1"
                     /translation="MEEIAFLKKIPILRDLDEEELGQFLKIARRVRFPKGQPIITEGQ
                     TGETLYIMEEGMVEISKTLVMGKGQENSRDRDKVLTKLSAEDHAIFGEVALFEQSKRT
                     ATVVALTDCLLLEISKADFMRLAEENPRVGYKITRNITQLLCSRLRKADEDTIKLTTA
                     LSIALSR"
     gene            complement(7553..>7845)
                     /locus_tag="VEL68_14375"
     CDS             complement(7553..>7845)
                     /locus_tag="VEL68_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37203.1"
                     /translation="IPERFVGKPVADLSSHLKEKFQALLIALLSERKGLVLDDILTDD
                     LSAIDQFIKRKFEEAEKDLPGRKDRVLVTLNPPDNTILTREHSAVVIARKRP"
BASE COUNT         1787 a         2120 c         2194 g         1744 t
ORIGIN      
        1 gcttcgttca agaccctgcc gaaggtggcc cgagatatct ccatcttcgc cgccgcttcc
       61 tcatggtaaa gcccctggta atcggccaga cggatggctt ccagttcatc catccggagg
      121 gatacctctt ccagggccat cagggggatt ccccgcggtt taaagtaata ggaattcgga
      181 ctgcaccgaa cgcagcggca tttcatcggg cgggccataa gggaaattat aagcatatgc
      241 tcataacctg tcaaggatat ttcgccactc ttagtttgtg cgaatcggcc ggagccctct
      301 tcggtcaatc cgcctgccat agctgtgatg atggagctag ggatcgatat ttccggccac
      361 cgatccaagt cgccggtgga attcatgaac gaaaaatttg atttcgtcat agccctgtgt
      421 gaccatgtgg cggaaagctg cccggttctt tccgggggga cccaaagaat tcatatgggt
      481 tttcccaatc cggcagccgc ttccggcagc gacaaagaaa ggctgatggc cttccgcaag
      541 gtgcgtaacc aaatacggga agaagtggtt gccttcttaa ccaaacgagg agcgcagggg
      601 cattgatgcg gaggtatgat aaattgcctc tagccggatc gatgaagtta tgggaaaata
      661 gaaataatct ttgtgaaaat tgccaaaaaa aaagaggtga tggatttgaa acctttgttt
      721 tttaagggca cgaggctcta tgggctggtg gccgtctttc tcgtctgcac ttttttcctt
      781 cctcccgatc cgatcgcccg agcaggggag acggaggtcc tgattctcca cacgaacaac
      841 gtagccggtt acctctttcc ctgtcccact tgaggggacc gtatgctggg cggtctggcc
      901 cggcgggtgg cttacgtgga aaaaatccag aggggaaatc agcgggcgct ggtggttgat
      961 tcaggggatc tttttttcaa ctttcaaagc tattccgatt cagaaaaggc aatgaagaaa
     1021 gcccaaatca tcgggcgagc ctaccgccat atgggtgcgg cggccgtgaa tgtaggatgc
     1081 ctggacctcc ttcagggagt ggatttcctg cgccaagaat tttcccaagg gcttcccctc
     1141 gtgtcggcga atctgctgga ccccgccagc aaaggcccca tctttcctcc ttatgtatta
     1201 aaggaaatcg gggagctgcg gatagccttc ttcgggcttc tctcgcctga gtcgggaccg
     1261 gagatcagcc cggttatccg ccaagccaat gaggggaaga tattgatcgg cgacccggtc
     1321 gaggcggcaa aagagaccct gcggaaactc cagggcaaag cggacctggt cattcttctt
     1381 tccgacctgg gcctctacaa ggatcaaatg gtggccctgg cagtaccggg aattcacttc
     1441 atcctgggag gtcatgaagg acgcttcacc cggaaagcgg ttcaggccgg catgacccac
     1501 attttccaat cctcccacaa gggaatgtat gtggggcagc tccaactggt cttggaaaat
     1561 ccctccaagc ccttcaagga tgacgggcaa gtccagtacc tgcaagaaag gatcgacgga
     1621 ctcgacctta atatccgggc cctggaaggg gccaagaaga ggcaacccgg aggggacacc
     1681 tccaacttcg accggtccat ccaggaaata acccggcaga gaaatgcctt gcaggaagaa
     1741 ctgaagcgcg caaaagaagt cggaatccaa gggaaccgtt tccttttcag gttggaagct
     1801 ctggagaaga ttcttcctga gaatgaagaa gtgaaaaagt ggatcagcgg cgcgggggtc
     1861 gataaggatt gaggatggac aattctccag attcctgcta atctttgtac aggttcatgg
     1921 gtctatcgtc ggtgatgccg accttagtaa tccgcacttt tttgcgctcc ccaaccaggc
     1981 ttatgattta gaaaatccga cagcctcagg gcaagggtaa gggggagatt ttttttcggg
     2041 gatgagagga aaggctgaat gaaaaagaat ctgaagtggg tcattccggt agtggccgtg
     2101 gtgggaatca tcgtcttcgg ttacctgaaa tttttcgcct ggaacccgga acgtcaggtc
     2161 ctggccatga tcaacgaccg gaagatcacc gtggcccagt tcgaccggga aatagccaag
     2221 atacccacgc cctttcagga tgttttccgg gaagagccca ggcagtactt ggagcaatgg
     2281 atcaccaagg aaatcgtgct tcaggaagcc agacgcctgg gggtacaacc cgatccagca
     2341 gccaaggggg acgacgtgga cctttccatc atgcagaacc tcttgaagaa ggaggtcctg
     2401 gacaagattc aggtcgaccc caaggaggtc gcggaagtct acaagcagca caaggatcag
     2461 atgggaaaga aatccctgag cgaagtgacc ccgatgcttg aagaggccat ccgggaagtc
     2521 aagggcaagg aaaaggtcga agaatatgtg gcctctttga agaacaaggc caagatcgag
     2581 atcaacgaga gccgcttgag ggatgtgacc gccctccccc cccccacgaa caccaaggag
     2641 gattttcaac aggcgatgaa gaacggaaag ccggccctgg tggacttcgg cgccaattcc
     2701 tgcatgccct gccggcagat ccgcccaatc ctgaaggaaa tcgaaaagga atacacgggg
     2761 aaagcttcga tcctgatcat cgacgtgtac aaataccagg acctagcccg ggaataccgg
     2821 gtccagctca ttcccaccct ggttttcttc aacaaggaag gaaaagaatc ttttcgccac
     2881 gtaggcgcct gggacaagga ctccatcgtg ggaaagctga aagaggctgg agccgcttaa
     2941 ccccaggagg ggactttccc agaacccggt ggaaggaagc gcggttttct acaacccaag
     3001 gtccgattga ttaaaattta ataattttaa atgcttgaat ccctccaatc cctcgaaggg
     3061 gtgggcttgg gggatttttt ttcctaaagg aagacccctc gggcgggaaa aggggccaaa
     3121 atgcagcccg ttcaccgatg gtctcggaaa tgaccttgac aaattctttt tttaccatta
     3181 ttttagaaat gttttctgga gggataacct gaacttcaga gggactgcct taagccctaa
     3241 ttcatcaagc cctcaaaaag aataccagaa tttaatttaa ctacatttgg aggaggttga
     3301 acatgcaccg gggaagtatc ggtctgaaat cggttttggt actgtctatc cttattatat
     3361 actcgattgc cggggccgcg ttggcgctgg gtcagtcgga taaggataag gatgccctcc
     3421 cccgtcccga gaggggaatt gccatttaca cggagttttc cggggtcatc gtgcctcccg
     3481 gcgaatccgt ccgcatggag ctcaccgcgg acaacaaggg aaaacgggac gagaatattt
     3541 ccatgaagat cagcgaggtt cccaaaggct ggagggcttc cctcaaggcc cccaatttca
     3601 gcgtcgatgc ggtgccagtg ccggccacga agacccgaac aatgaccttt ctggctgagc
     3661 cggataaaac ggtgaagccc gggaattatc tgttccagat cgacgcgcag accgaggacg
     3721 gaaaattcac ctccgtgcag aagatcaatg tgaccgtccg ggagaagact ccggggcagg
     3781 aagactttac cgtgaccacc tcctacccgg tcctgcaggg ccagacggat gccaagttcg
     3841 aattttccct ggaagtcaac aacaagagcg aagtggacaa gagcttcaat ctttcggccc
     3901 aggctccggc caaatgggaa accaacttca agccggccta cgagcagaaa cagatttcca
     3961 gcttccggat caagggaggg tccagccaga cggtgaccgt ggaagtcacg ccccccaggg
     4021 acgcgcagtc aggttccttc ccccttctgg tcagcatctc ctccggggat aagaaacagg
     4081 acgtgaagct cacggtcgtt ctcacgggta tttacaaact ggatgcgggc acccccaccg
     4141 gcctcctctc cctggaggcc ctggcgggca agccggccaa catgtccgtc ttcgtgaaga
     4201 acaccggttc ggcggtcaac aagaacatat ccttcagctc cttcaagccg gagaactgga
     4261 aggtggaatt caaaccggaa aagatcgagg ccctcgagcc caatgccctg aagcaggttg
     4321 aagtcatcat taccccggcc ggccaggcct tagtgggtga ctattccgtc ggcctttccg
     4381 tggacgggga gaagggcagc agcaagaccg tagaattgag ggtatcggtg aaaacttccg
     4441 ccgcctgggg ctgggtcggg atcatcatta tcgtattggt catcgccggc ctgggcgggc
     4501 ttttcctctg gctggggagg agataacgtg aacgggcaga cagtcatcga aacccaggac
     4561 ctgaccaagg tctacgggga acagacggcg gtaaaccgcc tgaccctcaa gatcaaccag
     4621 ggtgaggttt tcgggtttct ggggccgaac ggggccggca aaaccaccac cctcctcatg
     4681 ctcctgggac tcagcgagcc cacttcgggg aaagcctggg tgtgcgggtt cgaccccacc
     4741 cgggaggcgt tgaaggtcaa gcgggtggtg ggctatcttc cggagaacgt gggtttctat
     4801 gacgacatca atgccgtcca gaacctcctg ttcatcagcc ggctgaacgg aattccggat
     4861 catgtagccc tggccaagat cgaagagatg ttaaacctgg tggatctcaa acccgagccg
     4921 aaaaagaaag tcggggagtt ctcccggggc atgcggcagc gtttggggat tgcggaagtc
     4981 ttgctgaaaa atccccaggt cgtctttctt gacgaaccga ccctgggcct ggaccccgac
     5041 ggttcgcttc agatgctgaa tctgatccag tccctcagcc gggacaaaaa actcaccgtc
     5101 ttcttttcct cccacctcct agaccaggtc cagaagattt ccactcggat cggaatcatg
     5161 ctcaaaggga gcatggtcgc cgtgggacgg atcgaggacc tggccaagga aaagcttgga
     5221 ttggaagaag ggaaatacac cttggaagag ctctacatga agtactttaa ggaggggcag
     5281 ccgtgaaagg aatgattacc atctgccgca aagagctggc cgaccacttc agcagcaccc
     5341 ggtttaccct ccttttctac ctgatcctca tggtgagcat ggtcatcgcc tttatggtgg
     5401 ggaccgggct gaaggaggag ctgaaggatg tggccaagcc caccttcgtg tttctcatgc
     5461 tcttcacctc gccggggaaa tttctttccc tgatccagtt catctccatt tttggaccga
     5521 tcatcggcat tgtgctgggg tttgacgcag tcaaccggga gcggagtgcc cggacgttga
     5581 gcaagctggc ttcccagccc attttccggg actacatcat caatggaaaa ttcctggccg
     5641 gtctggtcac catcgtcatt atgcagctgg ccatcgtgct gatgatctcc ggtctggggc
     5701 tgctcctcat cggggtggtg cccggacccg aagagatcta ccgcctgatc atctatctgg
     5761 tgatcagcat cttctacatc ggtttctggc tggggatttc catccttttc tcggtcagtt
     5821 tccggagcat ggccacctcg gccctggcct caatcgcctg ctggatcttt ttcttcttct
     5881 tcgtgcccat gggtgcgggg gtgttggccg atgtgattgc ccccttcgat ccggcgcagg
     5941 cctcgcagac cccggaactg gtcatcaaaa acgagcaaac ccggaaggtc atttccctcg
     6001 tttcccccat gaccctgtac agcgaggcca cgaccatcat cctggatccc ctccggaaga
     6061 cgacccagtc gctcattctg atgggaccgt ttgaacggtt gtccatttcc cgcttccaga
     6121 atcctctccc cctttcggag agcgtctaca tcgtcctccc ccacctcatc accctgatcg
     6181 cgatcaccct ggtgtgcttc gccatctcct acgcggtctt catgcgccag gagatccgat
     6241 caacgtagaa aagcaggttc caggattcag gtctcaaggc caaaagcccc cttcctcttt
     6301 cgagggaggg ggcttttggt tgggtttctc ggtggtgaaa tcttagggct ttaattccac
     6361 cttgatctcc ggcttgtgca ggatggtttc gtggacgaaa caggtttcgg ccatgcgcag
     6421 gagccctttc ttttccgcct cggggatgtc gtggggataa tggagctcgg ctttgatgga
     6481 cccgaagcga gcggggtctt gggcgtaagt ccaatccaat aaaactttca tcccctcggt
     6541 gggaaggttg tgccgcttgc agaaccgaac ggcaaatact ccgatgcaat ttcccagaga
     6601 ggcgacgaaa agctccgccg gggtcatggc cgtatccgct ccgccttcct tttgctgctg
     6661 atccgtaacg acttcatgcc ctcggcattt ggctctgaac aaaaaatcct tttccaggtt
     6721 cacttccatt ttcatgagtt ttctcccgaa agagtgtcag tgtgagtgcg agtgtgagta
     6781 acaacaatgc ctacccccat cccctccccc ctcggaggct gtgtcgtaat cccctgttcg
     6841 cccttcgaca ggctcaggct tcgtcgcgag ctcagccgag cgggcgaacg tctaagtagc
     6901 tgaattttaa tgtgctcata atccgttcgt ggtgagctcg tcgaaccatg aacggattac
     6961 gacacaacct ctcgcggggg agggtcgggg tggggggcaa ctccgcaatt cgcaatccga
     7021 aatccgcaat cgtcgtttac cggctcaacg caatcgacaa ggctgtcgtg agcttgatcg
     7081 tatcttcgtc cgccttccgc agccgggagc agagaagctg ggtgatgttc cgggtgatct
     7141 tgtaacccac gcgggggttc tcctcggcca gtctcataaa atcggccttg gagatttcca
     7201 gcaggaggca gtcggtcagg gcaacgacgg tggcggttcg tttgctctgt tcaaacagcg
     7261 cgacttctcc gaatatggcg tggtcttcgg ccgagagctt cgtcaggact ttatcccggt
     7321 cccgggaatt ctcctgccct ttgcccatga ccagggtctt ggagatttcc accattcctt
     7381 cttccatgat gtacagggtc tctccggtct ggccttcggt gatgatgggc tggcctttgg
     7441 ggaaccgaac ccggcgggcg atcttcagga attggccaag ctcctcttcg tccaggtctc
     7501 ggaggatggg gatttttttc agaaaggcaa tctcttccat ggctttttcc tctcagggac
     7561 gttttcgggc gatgaccacg gcggagtgct ctcgggtaag aattgtgttg tccggcgggt
     7621 tcagggttac caggactcgg tcctttcttc ccgggaggtc cttttccgcc tcctcgaact
     7681 tgcgcttgat gaactggtcg atggccgata agtcgtcagt cagaatgtcg tccaaaacca
     7741 aaccttttcg ctcgctgaga agagcaataa gcagagcctg gaatttttcc ttcaggtggg
     7801 acgataagtc ggcgacgggc tttcccacaa agcgttccgg aatct
//
LOCUS       DASPRR010000390         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_407090, whole genome shotgun sequence.
ACCESSION   DASPRR010000390 DASPRR010000000
VERSION     DASPRR010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407090"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VEL68_14380"
     CDS             complement(<1..1427)
                     /locus_tag="VEL68_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXZ37204.1"
                     /translation="MVRRFLRPGFFLISLASLIGLSPAAKGAEVEVGQVVVTATRTEV
                     EISDSPQSISVITKEEIMNSPDRTIPEVIQRAAGVQINQTGPIGGLSTASIRGSEAQQ
                     VLIMIDGRRINDAQNGQFDLSSLPLTKDEIERIEVLRGGASALYGSDALGGVINIITK
                     SPTKEPYARVSSFFGRFNTQEYSLSSRWKPGPLGYGVFASWGRSDGYRPNGDYNAWTV
                     GGDLSYDLPWKGELKVSARNIQKEVGVPGTSTLTDPDDRQKDNITQLDLNYRDRIGSN
                     VTLNFRGFQNINRQTFDAGSQGPDVGPPIIHKSYATGGDFQTTAAIGNANLVTGGIEA
                     IQNRVDSDSVGVHEATTGAVYLQDEIEVAKALTATLGLRYDYHSVYQDQWDPRAAVLL
                     RLPWETRLRASVARSYRAPTFNDLYWPASAFTAGNPNLQPEKAWSYELGGEKRFGNLA
                     IFKVAGFYREVTDLIRWAPGTDFVWR"
     regulatory      complement(1452..1723)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1693..>2244)
                     /locus_tag="VEL68_14385"
     CDS             complement(1693..>2244)
                     /locus_tag="VEL68_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37205.1"
                     /translation="RVYETTVMVEKNPMSGAPRVTPVGKSRMGKTGPQKTIHIICGEG
                     SGVDSARRLLLRGYRVSLGVLNIGDSDQKIGKTLGLAMALEEPFSPISEAAYRENLIL
                     MEKADLIIVERFLVGKGNLLNLRAAVDALGKGKRIIALENPLEMDFTGGEARAYYQRL
                     KEGGAVFIPDHSRLLEEVEKVLR"
BASE COUNT          446 a          658 c          615 g          525 t
ORIGIN      
        1 cgccatacga agtcggtccc cggcgcccag cggatgaggt cggttacttc ccggtaaaag
       61 ccagcgactt tgaagatcgc cagattaccg aatctcttct ccccccccaa ttcataagac
      121 caagccttct cgggctggag gttcgggttg cccgccgtaa acgcggaggc cggccagtag
      181 aggtcattga aagttggggc gcgataggag cgggccaccg aagctcgtaa gcgagtttcc
      241 cagggtaagc gtaagaggac cgcggcccgc gggtcccact gatcttggta gacggaatga
      301 taatcgtaac gaagcccaag ggtggcggtt aatgctttgg ccacctcgat ttcgtcttga
      361 agataaacgg cgcccgtcgt ggcctcgtgg acacccaccg agtcgctgtc aacccggttc
      421 tggatggctt cgattccgcc ggtgacaagg ttggcgttac cgattgctgc cgtggtctgg
      481 aaatccccgc cggttgcata gctcttatgg atgataggcg gtcccacatc aggtccctgg
      541 ctccccgcgt cgaacgtttg ccggttgata ttctgaaatc ccctaaaatt caaggtcaca
      601 ttggagccga tcctgtcccg gtaattcagg tcaagctggg taatattatc tttctgccga
      661 tcgtctggat cggtgagggt gctcgtaccg ggaacgccga cttccttctg aatgttccgg
      721 gcggaaactt tgagctcccc tttccaggga agatcgtagc tcaggtctcc cccaacagtc
      781 caggcattgt aatcgccgtt gggacggtat ccgtcggatc ttccccagga ggcaaacact
      841 ccgtaaccga ggggccctgg cttccagcgg gagctcaggg agtattcctg ggtgttgaac
      901 cggccgaaga acgaggaaac ccgggcgtag ggctccttgg ttggagactt ggtaatgata
      961 ttgattaccc cgcccagggc atcggaccca taaagggcgg aggcaccacc gcgaagcact
     1021 tcgatgcgct cgatttcgtc cttagtcaag ggcaggctgc tgaggtcaaa ctggccgttt
     1081 tgcgcatcat tgatccggcg gccgtcaatc atgatcagca cctgctgcgc ttccgaaccg
     1141 cggattgaag cggtagagag accgccaatc ggcccggttt gatttatctg gacccccgcg
     1201 gctcgctgga tgacctcggg aatggtccgg tcggggctat tcatgatctc ttccttcgtg
     1261 atgacgctga tcgactgggg ggaatcactg atctccacct cagtccgggt ggccgtgaca
     1321 acgacttgcc ccacctcgac ttctgccccc ttcgccgctg ggcttaggcc aataagcgag
     1381 gccagactga ttaaaaaaaa ccctggccgt aagaatctcc gtaccacctg tttcctcctt
     1441 tcccgcgaaa ggtttgaaag ctctctgaag gatcaggtct actggcttcg ggcttcctac
     1501 tccccacgcc ttcccggaca atgtccagtg gctgaaaata gctttcagcg atcagcgttt
     1561 agctttcagc aaaataaaga atgccttcgc tgactgctat ttgctgatag ctgatggctg
     1621 tttttttgtg gttttcgttc ccatcacagt tgcggggcag cggtggaatc gcaccacctt
     1681 ccgtttgaac cttcagcgca aaactttctc tacctcctcc aatagtcggg agtggtcggg
     1741 aatgaacacc gcccctccct ctttcaggcg ttgataatag gccctggctt ctcctccggt
     1801 aaagtccatc tccaggggat tttccagggc gatgatcctc tttcctttcc ccaaggcatc
     1861 gacggcggcc cgcaggttga gtaggttccc tttgcccacc agaaagcgct cgacgatgat
     1921 gaggtcggcc ttctccatca aaatcagatt ctcccggtag gccgcctccg agattggcga
     1981 aaaaggttct tctagcgcca tggccaatcc cagggtcttc ccgatctttt ggtcggaatc
     2041 accgatattc aacacgccca gcgaaacccg atatccccgg aggaggagac gccgggcaga
     2101 atccactccg cttccttcgc cgcaaatgat gtggatcgtt ttctgcgggc cggtttttcc
     2161 catccgggat tttcccacgg gggtaactcg tggggctccc gacatgggat ttttctccac
     2221 catcaccgtg gtttcataga cccg
//
LOCUS       DASPRR010000391         8464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1056152, whole genome shotgun sequence.
ACCESSION   DASPRR010000391 DASPRR010000000
VERSION     DASPRR010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056152"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VEL68_14390"
     CDS             complement(<1..583)
                     /locus_tag="VEL68_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ37206.1"
                     /translation="MAWEFVLYNKEDGIGTITMNRPEAMNALGGGMRQEILEALETAA
                     SDREVRVIVITGSGKAFCAGGDVKEFVSGTSRADTDIPLDQRPTRDKIVLLIQSTGKP
                     VIASVNGVAAGGGCNLALACDMRIASDRARFGEVFVRRGIHPDWGGTWFLPRLVGYAK
                     AAELIFSGELIDAQEAFRIGLVNKLVPHEELPKA"
     gene            complement(787..1632)
                     /locus_tag="VEL68_14395"
     CDS             complement(787..1632)
                     /locus_tag="VEL68_14395"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXZ37207.1"
                     /translation="MKNRITKILGSEFPIIQGAMRRISLGELAAAVSRAGGFGIIGAS
                     GMEGNALRAEVRKARSLTDRPFGLNIPIYRPNAFEALEIAVEEGVKTITTSAGDPGKV
                     ILRVKEAGLRILHVVATVEMARKAEGAGVDAVIAVGAEGGGHVGRDEISTLVLIPQVV
                     DAVRIPVVAAGGIGDGRGLVAAFALGAEGVQMGTRFLATQQSPVPSSYKQAILKAKDD
                     GTMIAARKGYPIRVLKNRPPWPFGKWMREEPLRRRSTSLWTVFPIKEKVERGKGSCRQ
                     GRLRD"
     gene            1791..3647
                     /locus_tag="VEL68_14400"
     CDS             1791..3647
                     /locus_tag="VEL68_14400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HXZ37208.1"
                     /translation="MGENPDYIFRYSVEAPAAGSQRDILVEMIRTQLESLFGLLCLYQ
                     EGEEVKVDGFRLMNEGERIHPIFSSPVSPEKSSSSPPSPVPKKGEAAFQSIRNAELYE
                     PRIPHIKRVLESLLSVIVLESEGKPVRVDGFRLKDLNHWLVPSAGDPAEVLDHLATRC
                     DCNCSFCYLRGNPPSLALDQPKRKSEEEWEEVRTRLKYFSPPGKRSLFPSLGSSYEIL
                     SHPKALDLLRELRKKTDRPFRISTNGNRLTRDFIRELATLNPVYLYLSLNSSSPRRRR
                     EIMGAANPDTAIHSLPLLKEQGIPFAVVIVPWPFPSKEEMLSDLKETVAHAGRHDAHL
                     VEVSLPGYTQYFSPELLFDHQEVWSSIVSGVRTLRQEVQCPVLVKPSLYEENLHERKF
                     NLPAVIGLIKNSPATLCGMQKGDLVLSIGGLRVSSRPQARDFLHLHHQNRTPSLSLSV
                     QRGREILELNLLPGQHLYPYAPETDHHLGMIFMGAGFRPSLLEDLKSLIKIHEARNVL
                     LLSSRLVKPIFLQLMRESSLFGGIQIQVEVPENRFFGGNICMGDLLVIRDFIQAIKEY
                     LRKNPSPDLIIIPSSPFSLGQWGRDLEGRVFAEIERSVGLPVALLDCEPIYD"
     gene            3799..4911
                     /locus_tag="VEL68_14405"
     CDS             3799..4911
                     /locus_tag="VEL68_14405"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HXZ37209.1"
                     /translation="MSAEIKGPQDLEKEVIKADLCTLCGACVGLCPYFRAHRGRIVLM
                     DNCNLSQGRCYFFCPRTSSDLEAVNQALFGAPYPGNSLGTHRQVTMAKSLDKTSLAKG
                     QYGGVVSALIALALKKKMIDGAVLTQRGKDLLSSGTLAKDKRQVLACAGSNYIASPTL
                     EAFNKGVQSFPKKLAAVGIPCQVLALGKMRINPLENKNQIEKLQLVIGLFCTWALAYQ
                     EILQFLKKGYPIEKIGKMDIPPPPANILQLQLPEGMKSISLDHIRKFVRPTCSVCLDM
                     TAEFSDLSVGAVEGIPGWNTVIVRTVKGEELLEEAKKARMIEVSEVPRANLKHLEEAS
                     LLKKKRALENIVKRTGSKDNLMYLRGADALRPLLPQ"
     gene            5002..6918
                     /locus_tag="VEL68_14410"
     CDS             5002..6918
                     /locus_tag="VEL68_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ37210.1"
                     /translation="MSVIKKNAPGSNELLMGNEAIARGALEAGVQFAAAYPGNPSSEI
                     IGTLSQVAKEMGIYVEWSVNEKVALEAAASASFAGLRSMSAMKQNGVNVASDFILNLN
                     LSGVGTGGLVLVAADDPAALSSTNEEDSRFISKLGDLPLLEPATFQEAKDMAKWAFDL
                     SESINSLVIVRSVSRISHARGNVLLGELPKEKKTARYDTSKIFIPVAAPLRHLSLHLK
                     LKKVQEMYESSPFNWYVGPEKPELLMVTAGSGWFYSQEAVRTLSLQDRVGVLKIGTAW
                     PLPSKLLAKHMANAKRILFVEEVDPFLENNVKELAAEWSPEIGSWTFYGKSSGHVYPF
                     GEINPDLIINAVSKVMDKPYAPREANYGKKALEMTKSLVPHRDLGFCAGCPHRATFWS
                     IKNALKLDGRGGFVTGDIGCYSIGLGPAGFYQLKTMHAMGSGTGLATGFGQLKQFGFN
                     QPVLSVCGDSTFFHAAIPALINGVYNQSNFLMVVLDNSATAMTGFQPHAGTGETAIGS
                     PAPVVNIEKLCSSLGVRVETCDPFDLQDTTEKVLDLVNQEGGVRVLISRRKCALVKAK
                     GEKAAYKVHVDPEKCKGEACGCARLCTRIFKCPGLIWNQEKGKSKVDEVICSGCGVCT
                     DICPQSAIIREENR"
     gene            6915..7523
                     /locus_tag="VEL68_14415"
     CDS             6915..7523
                     /locus_tag="VEL68_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXZ37211.1"
                     /translation="MNLSKDPLNLIIAGVGGQGNVMASQLIGRALVRKSYYVTIGDTY
                     GASQRGGAVMSHLRISQDSQFSPLIPEGQADLIVALEPVEALRVIAQYGNPSTSAVIN
                     SRPIYPTAVTVGEAQYPSLDKIKETIHELARKSWFVDATEIALSLGAPIFTNIIMVGA
                     VAGTGLLPLGRKTYEEVIHESFPGAHLEVNLKAFAKGVELIH"
     gene            complement(7662..8108)
                     /locus_tag="VEL68_14420"
     CDS             complement(7662..8108)
                     /locus_tag="VEL68_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXZ37212.1"
                     /translation="MAVSLEPMTKVSDKLKELLNKAIAREMQVSIQYMWQHVQVMGVK
                     AVAVQDKFKQTAVAEMKHAEKIAERLWYLGGTPTTKPEPIMVGKSLKEFLELDTKAEE
                     EAIMMYKGIIEQAQKEGDVTTAFIFKEILEDEEEHHDLFTTMLEEV"
BASE COUNT         1843 a         2535 c         2187 g         1899 t
ORIGIN      
        1 tggctttagg cagttcttcg tgggggacca gcttattgac caagccgatc cgaaaggctt
       61 cctgggcgtc gatcaattca ccggaaaaaa tcagttccgc ggctttggca tatccgacca
      121 gtctgggcaa gaaccaagtc cctccccagt cgggatgaat cccccggcgc acgaagactt
      181 ccccaaaccg ggcacggtcg ctggcgatgc gcatgtcgca agccagcgca aggttgcatc
      241 ctcctccggc ggcgactccg ttcaccgagg cgatgaccgg ttttcccgtg gactgaatta
      301 gaaggacgat cttgtcccgg gtgggccgtt ggtccaaggg gatgtcggtg tcggcccggg
      361 aggtcccgga cacgaactcc ttcacatccc cccccgcgca gaaggctttt cccgagccgg
      421 tgatcacgat gacccggacc tcccggtcgg atgctgccgt ctccagagcc tccaggattt
      481 cctgcctcat gcccccgccc agggcgttca tcgcctccgg ccgattcatg gtaatcgtcc
      541 cgattccgtc ttccttattg tacaaaacaa attcccacgc catctcatcc tccgcccatt
      601 caagattcga tgatgattct gtagtcaatg gtctatttcc tgttagttct tgtctctagc
      661 ctattaccaa taactcatga cctacaacct tttattgcta aaattgtttt cccagccttt
      721 ggacgaccga cttcgcttca gccacgatgc catggattac ctcctccacg gtcttgatgt
      781 cttggatcaa tcccgcaatc tgccctgccg acatgagccc ttccccctct ccaccttttc
      841 ctttatcgga aagactgtcc acaaagacgt tgatctccgc ctgagtggct cctctctcat
      901 ccatttcccg aacggccagg gcggccgatt cttcaggact cgaatgggat accctttccg
      961 ggcggcgatc atggtcccgt catcctttgc cttaaggatg gcctgcttgt acgaagacgg
     1021 caccgggctc tgttgggttg ccagaaaacg agtccccatc tggaccccct cagccccgag
     1081 ggcaaaggcg gccaccaggc cacgtccgtc tccaatccct ccggcggcca ccaccgggat
     1141 gcgaacggcg tcaactacct gggggatgag aaccagagtg gaaatctcat cccggcctac
     1201 gtgtcccccg ccctccgccc ccacagcgat cactgcatcc actcctgccc cttcggcctt
     1261 tcgggccatt tccacggtcg ccacgacgtg gagaattctc aaaccggcct ccttcacccg
     1321 caggatgact ttgcccggat cgccggctga tgtagtaatc gtcttgacac cctcctccac
     1381 tgcgatttcc agggcttcaa aagcgttggg ccggtagatg ggaatgttca agccgaaagg
     1441 ccggtcggtg agagagcggg ccttgcggac ctccgctcgc agggcgttcc cctccatccc
     1501 agaggccccg ataattccga agcctccggc gcgggacacg gcagccgcca gctcccccag
     1561 gctgatccgg cgcattgccc cctggatgat gggaaattcg gaacccagga ttttcgtaat
     1621 ccgattcttc atcgtcacct ccccaaagaa caaaatcccg gcaggaaatc cacaacccat
     1681 ttctgtccaa caggaagata gacctttcta tttgccgtgt caaccgcgga gatttaaaag
     1741 atttaaaaac attgaatcct atctcttctt tgcggtagat tgaaaatgtg atgggcgaga
     1801 accctgatta tatctttcgg tattcggtgg aagcacccgc ggccggcagc cagcgtgata
     1861 ttctcgtcga gatgatccgg acgcaactgg agagcctctt tggattactt tgcctgtatc
     1921 aggaagggga agaggtgaag gtggacggct tccggctgat gaatgaaggg gagagaatcc
     1981 atccgatctt ttcctcgcca gtctccccgg aaaaatcttc gtcttctcct ccgtctcccg
     2041 ttccaaagaa gggagaggcg gcgtttcaga gcatccggaa tgcagagctt tacgaaccgc
     2101 gaatccccca tatcaaacgg gtcctagaga gcttgctctc ggtcatcgtc ctggagtcgg
     2161 aagggaaacc ggtaagggta gatgggttcc gcttgaaaga cctgaaccac tggctggttc
     2221 catccgcagg agacccagcg gaagtcttag accacctagc tactcgctgc gactgtaact
     2281 gcagcttctg ctacctccgg ggaaatcccc cttctcttgc gctcgatcag cccaaaagaa
     2341 agagcgaaga agaatgggag gaagtccgaa cgcgcctcaa atatttttct cccccgggga
     2401 agaggtccct ctttccctcg ctgggaagct cctatgaaat tctctcccac cccaaggctc
     2461 ttgaccttct ccgggagctc cggaaaaaga ccgaccgccc ctttcgtatc tccaccaacg
     2521 gaaaccgcct gacccgcgat ttcatccggg agcttgctac cctgaatccc gtttatctct
     2581 atctttcatt gaactcctct tccccgagac gccgcaggga gatcatggga gccgccaatc
     2641 cggacacggc cattcactcc cttccgctcc tcaaagaaca ggggattccc tttgccgtag
     2701 tcatcgtccc ctggcctttt ccatccaagg aagaaatgct ttctgatctg aaggaaacgg
     2761 tagcccatgc gggccgtcac gacgcccacc tggtggaagt tagcctaccc gggtatactc
     2821 aatatttctc gccggaactg ctatttgatc accaggaagt ttggtcttcc atcgtctccg
     2881 gggtccggac gctgcggcaa gaggttcagt gccctgtact ggtcaagccc agcctgtatg
     2941 aagaaaatct ccatgaaagg aagttcaatc ttcctgccgt gatcgggctc ataaagaatt
     3001 ctccagcgac tctctgcgga atgcaaaagg gggatttggt tctctccatc ggaggactta
     3061 gagtttcctc ccgtccgcag gcccgggatt ttctccatct ccaccatcaa aatcgcactc
     3121 cctccctctc cctttccgtt cagcgaggca gggaaattct cgaattgaat cttctccccg
     3181 ggcagcacct ctacccttat gccccagaaa ccgatcatca cctgggaatg atcttcatgg
     3241 gagccgggtt ccgacccagc ctcctggaag acctgaagtc cctgattaag attcatgaag
     3301 ccagaaacgt gcttcttcta tcctcccgtc tggtgaaacc catcttcctc cagcttatga
     3361 gggagtcttc ccttttcggc gggattcaga ttcaggtcga ggtcccggag aatcgctttt
     3421 tcggggggaa catctgcatg ggggacctgc tggtcatccg tgatttcatt caagccataa
     3481 aagaatacct aagaaagaac ccttccccgg acctgatcat cattccctct tctccctttt
     3541 ccctgggcca gtggggaaga gacctggaag gaagagtctt tgccgagatc gaaagatcgg
     3601 taggcctccc cgttgccctt ctggactgcg agcccattta tgattaatct cccctctcct
     3661 catcctgata tttttaattg acaaaaatga gaatatgaaa taaagcatag tcaatttccc
     3721 cggtagctct taaaattctc ctggatggga aattaataat ctattcattc tgtaaagagc
     3781 cagaaagggc aagaacagat gagcgcggaa atcaagggtc cccaggacct ggaaaaagaa
     3841 gtaatcaaag cagacctctg caccctctgc ggtgcttgcg tgggcctctg cccctatttc
     3901 cgggcccatc gaggccggat cgtcctcatg gacaactgca acctttccca ggggcggtgt
     3961 tacttttttt gcccccggac ttcttcggac ctggaagcgg tcaaccaggc cctgttcgga
     4021 gcgccgtacc cgggcaattc gctgggaacc caccgccagg tgaccatggc caaatctttg
     4081 gacaagacct ccctggccaa gggccaatat ggcggagtgg tctcagcgct cattgccttg
     4141 gctttgaaga aaaaaatgat cgacggagct gttttgaccc agagggggaa agaccttctc
     4201 tcttccggaa ctctggccaa ggataagagg caagtcctgg cctgtgccgg ctcgaattac
     4261 atcgcctccc ccaccctgga ggcgttcaac aagggcgtcc agagtttccc caagaaactc
     4321 gccgccgtgg gcattccctg ccaggtgctg gccctgggaa agatgaggat caaccccctg
     4381 gagaacaaga accaaatcga aaagctccag ctggtcatcg gcctattctg cacctgggct
     4441 ctggcttacc aggagattct gcaattttta aaaaagggct atcccataga aaaaatcggc
     4501 aagatggaca tccccccgcc cccggccaat attctccagc tccagctgcc ggaagggatg
     4561 aaatccattt ccctggatca catccgcaag tttgtccggc ctacctgcag cgtttgcctg
     4621 gacatgaccg cggaattttc cgatttgtcc gtaggggccg tggagggaat ccccggctgg
     4681 aacacggtca ttgtccgcac ggttaagggg gaggagcttc tggaagaggc caagaaggcc
     4741 agaatgattg aagtgtcgga ggtgccccgg gccaacctga agcacctcga ggaagcctct
     4801 ctgctgaaga aaaagcgcgc cctggagaac atcgtcaaac gaacgggcag caaggataat
     4861 ttgatgtacc ttcggggagc agacgccctg cggccacttc tgccccaata agtttcgcgt
     4921 ctcgggtttc gagtttaatg ttttcgtttt cgaacccgaa acctaaaact cggaactcga
     4981 aactgttttt aggaggaatg tatgtcggtg atcaaaaaga acgctcccgg ctccaatgag
     5041 ctcctgatgg gcaatgaggc catcgcccgc ggagccctgg aggcgggggt ccagtttgcc
     5101 gccgcctacc ccggtaatcc ttcatccgaa atcatcggca ccctctccca ggtggccaag
     5161 gaaatgggaa tctatgtgga gtggtcggtg aacgagaagg ttgctctcga agctgcagcc
     5221 tcggcctcct ttgccggcct gcggtccatg agcgccatga agcagaacgg ggtcaacgtg
     5281 gcttcggatt tcatcctcaa cttgaacctt tccggcgtcg ggaccggcgg gctggttttg
     5341 gtcgctgcgg acgatcccgc ggccctctct tccaccaatg aggaggattc ccgctttatc
     5401 tccaagctgg gggacctccc cctcctggag ccggccacct tccaggaagc caaggacatg
     5461 gccaagtggg ccttcgacct atccgaatcc atcaacagcc tggtcatcgt gcggagcgtg
     5521 tcgcgtatct cccatgcccg ggggaatgtc cttctgggcg agttgcccaa ggaaaagaag
     5581 accgcccgct acgacacctc caagatcttc atccccgttg ccgctcccct tcgtcatctg
     5641 tccctccacc tgaagctgaa aaaggtgcag gagatgtacg agtcttctcc cttcaactgg
     5701 tacgtgggcc cggagaagcc cgagcttctc atggtcaccg cgggaagcgg ctggttttat
     5761 tcccaggagg cggtaagaac cttgagcctt caggaccggg tgggggtcct caagatcgga
     5821 accgcctggc ctcttccctc caagctcctg gccaaacata tggccaatgc caaacggatt
     5881 ctcttcgtcg aagaagtgga ccctttcctg gagaacaatg tgaaggaact ggccgccgag
     5941 tggtccccgg agatcgggtc ctggaccttt tacgggaaga gctccggcca tgtctacccc
     6001 ttcggggaga ttaacccgga cctgatcatc aacgccgtat ccaaagtcat ggacaagccc
     6061 tatgccccca gggaagcgaa ctacggaaag aaagccctgg agatgaccaa gtccctggtc
     6121 ccccaccggg acctgggatt ctgcgccggc tgtccccacc gggccacttt ctggtcgatc
     6181 aagaatgccc tcaaacttga cgggcgcggg ggcttcgtga ccggagacat cggctgctat
     6241 tccatcggcc tgggcccggc cggtttttac cagctgaaaa ccatgcacgc catgggatcg
     6301 gggacgggcc tggccaccgg ctttggacag ttgaagcagt tcgggttcaa ccagcccgtg
     6361 ctttcggtct gcggcgactc cacctttttc cacgccgcca tccccgccct gatcaacggg
     6421 gtgtacaacc agtccaattt cctcatggtc gtcctggaca acagcgccac ggccatgacc
     6481 ggctttcagc cccatgccgg gaccggggag acggccatcg gatcgccggc tcctgtggtg
     6541 aatatcgaaa aactctgttc gtccttgggc gtccgggtcg aaacctgcga ccctttcgac
     6601 ctccaagata caactgaaaa ggtcctcgac ctggtcaacc aggaaggagg ggtccgggtc
     6661 ctcatctccc gccgcaaatg cgccctggtc aaggccaagg gagagaaggc cgcctacaaa
     6721 gttcacgtgg acccggaaaa gtgcaaagga gaggcctgcg gatgcgcccg gctctgcacc
     6781 cggattttca agtgtccggg attgatctgg aaccaggaaa aaggaaaatc gaaggttgat
     6841 gaggtcatct gctccggctg cggggtctgt actgacatct gcccccaatc agccatcatc
     6901 agagaggaaa accgatgaac ctatccaagg accctttgaa tctgatcatc gccggggtcg
     6961 gggggcaggg aaacgttatg gcctcccagt tgatcggccg agccctggta cggaaaagct
     7021 actacgtcac catcggggac acctacgggg catcccagcg tggaggggcg gtcatgagcc
     7081 atctgcgcat ctcccaagac tctcagttca gccctttgat ccccgaagga caggcggacc
     7141 tcatcgtagc cttggagccg gtggaggccc tgcgagtcat cgcccagtac ggcaacccgt
     7201 ccacctccgc ggtgatcaac tcccggccca tttaccccac ggccgtaacc gtcggcgaag
     7261 cccaataccc ctccctggac aagatcaagg aaaccatcca cgagttggcc cggaaatcct
     7321 ggtttgtgga tgcgacggaa atcgcccttt ccctgggggc cccgatcttt accaacatta
     7381 ttatggtggg agccgtggcc gggaccgggc ttcttccttt aggccgaaag acatacgagg
     7441 aagtcatcca tgaaagcttc ccgggtgccc atctggaggt gaatctgaag gcttttgcca
     7501 agggggtcga gctcattcac tgaaattacg gattgccgaa taagcggagc cgaagtcaag
     7561 ttctgaatcc tctctgaaaa agaaagggct tccctgaatg gaagggaagc ccttttcttc
     7621 tcgcattgac ccaaaacttg aaactcgaaa cccgatactt tttatacttc ttcgagcatg
     7681 gtggtaaaga ggtcgtggtg ttcttcttcg tcttccagga tttctttgaa aataaaggcg
     7741 gtggttacgt ccccttcctt ctgagcctgc tcgatgatgc ccttgtacat catgatggct
     7801 tcttcttcgg ccttggtatc gagttccaaa aattccttta gagattttcc caccatgatc
     7861 ggttccggtt tggtcgtcgg ggttccccct aaataccaga gccgttcggc gatcttttcg
     7921 gcatgtttca tctccgcaac tgccgtctgt ttgaatttat cctgcacggc gaccgctttg
     7981 acacccataa cttgtacatg ctgccacata tactggatgc ttacctgcat ctcccgggca
     8041 atggctttgt ttaaaagttc cttcagcttg tcgctcacct tggtcatagg ttccaaagaa
     8101 acagccattt tgcacctcct ggttgggatt taaatccaca aaaatattgt acaccaatgc
     8161 caacccaaaa caacctgttt ttaattttcc ctcgctgcta gccaacaatg attccttgcc
     8221 cacccccagt ccttaaaaaa gagtctttcg aaactaagat ctctggaaag gaggggggat
     8281 ctcatggggt tcagggtgga gaaatttgga tcatcatcct gaggcggtgg ttcttccatt
     8341 ggatcgggcc tgtcctgggg ggattgaaac ggaaacatgc tttggctctc ttttatgctt
     8401 ggaggaatct cctctcaggc ctctccctgc ggaacatggg gaggagtttc atggaaggat
     8461 aagc
//
LOCUS       DASPRR010000392         3960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_743873, whole genome shotgun sequence.
ACCESSION   DASPRR010000392 DASPRR010000000
VERSION     DASPRR010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743873"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VEL68_14425"
     CDS             <1..999
                     /locus_tag="VEL68_14425"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ37213.1"
                     /translation="EIRMKFSRREFLKYSGIVAGASVLGMEGGTGMAMDKSRVIFFQT
                     EDRKQGVNTILKSLKGNPIKGKKVLIKPNFNTADPTPGSTHNETLVALVEEVWKIGAK
                     SVSVGDRSFPPTLDVMEKKGILPLLEKLQVQVTNFDDLKEKDWVEFKPKGSHWPEGFR
                     IARPVLESEYLISTGCLKTHQYGGIFTLSLKLHVGVVPTSRQGFEYMRQLHGSPHQRK
                     MIAEINEPFKPNLVIMDGIEAFVDGGPATGKRAQGNVFWASSDRVAVDAVGVSLLKHL
                     GGNEQIMKRKIFEQEQIARAVELGLGVSSPGEIELVAADANSREYCERVAAVLNQG"
     gene            1080..1892
                     /locus_tag="VEL68_14430"
     CDS             1080..1892
                     /locus_tag="VEL68_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37214.1"
                     /translation="MVQGAFLSFSLCQPDSSKSCGACCGLYNWEEHSRKTLLSMLEKR
                     SALFLSLGRDPEIFQEVCAREMFHSNPKLLEEVYNCEFLGFLDGGKKRVGCLLHPSVN
                     EGQDLRDHCFYGEEICAGHHCPSHSHLTPVERKSVVLALEDWYLYGLVITDIDLVKDF
                     FHQVQSRLGDSVREERMENFQVREALREFFTLKEYWKFASHRNRLGKYYFSCSEYQVA
                     RIEYEKNWRIKPSRFDKILISLESDFQTREDIFEAESIIERKITRFLRAYEG"
     gene            2021..2554
                     /locus_tag="VEL68_14435"
     CDS             2021..2554
                     /locus_tag="VEL68_14435"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXZ37215.1"
                     /translation="MMSSNTQERIKALEIALNNEAREKDFYLKHSKRTTNNMGKVMFA
                     TIASDEDEHYRRILGLHKRLIQEGKWPETVPIEVKGTEVKKVIQKVVDAVNATARADR
                     DDMEAVKIAIDFETKGEKFYGDLAKSVENPMEKNFYEFLASIEREHRLSLQDTLEYFE
                     DPAGWFRIKERLHVDGL"
     gene            2682..3569
                     /locus_tag="VEL68_14440"
     CDS             2682..3569
                     /locus_tag="VEL68_14440"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HXZ37216.1"
                     /translation="MRKVKKWIELGRTVFPPNLWLPAFSIVLGALMWAISINGILVHH
                     KLLSGGVSGLALVVYYLIPKLSIGLMVFLMNIPTFIMGWTMISGKFFAFSLLGMASFS
                     FFLTVTTWLQIAVENPLLACLFAGVISGVGSGLIFRAGGSGGGTGIIAMVLNRRFSVR
                     VGIVSFLLNSVPLVLGAFLIDLNSALYSIIYVYVSGSVMDRIITSFNERRSVWIISSQ
                     SSEVYGQILSKLRRGATLLAGKGAYTQSPVEVIYSVISPFELAKLKDLVITIDPKAFI
                     IINETQEVIGKGFDQPGSF"
     gene            3660..>3960
                     /locus_tag="VEL68_14445"
     CDS             3660..>3960
                     /locus_tag="VEL68_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HXZ37217.1"
                     /translation="MDEKRRNNLLRRGKMLQGIRQYFLGEDFVEVETPYLVPSPGMEP
                     HLVALEVNVQFPDKEKRKMYLHNSPEYCMKKLLGQGWDKVFQICRVFRDEEVSRTH"
BASE COUNT          957 a          971 c         1113 g          919 t
ORIGIN      
        1 gagatcagga tgaaattcag ccggagagaa tttctcaaat attccgggat cgtcgcggga
       61 gcatccgttt tgggaatgga gggaggtaca ggcatggcca tggataagag ccgggtgata
      121 ttttttcaga cggaagaccg caagcagggc gtcaatacca tattgaaatc tctgaaaggc
      181 aatccgatca aggggaagaa agtcctgatc aagcccaact tcaacaccgc cgacccgacg
      241 ccggggtcca cccacaacga aacgcttgtg gccctggtag aggaggtctg gaagataggg
      301 gccaaatcgg tgagcgtggg ggaccggagc tttcccccca ccctggatgt catggaaaaa
      361 aagggaatcc ttcccctttt ggagaagctg caggttcagg tcaccaattt tgacgatttg
      421 aaggaaaagg attgggtgga attcaaaccc aagggcagtc actggcccga agggttccgg
      481 attgcccggc cggtcctgga atcggagtat ctcatctcta ccggatgcct gaaaacccac
      541 cagtatggag ggatcttcac cctttccttg aaactccatg tgggagtggt gccgacctcc
      601 cgccaggggt ttgaatacat gcgccagctg cacggctcgc cccatcagcg gaagatgatt
      661 gccgagatca acgagccctt caaacccaat ctggtgatca tggacggaat cgaagccttt
      721 gtggacggcg gaccggccac cggaaaaagg gcgcagggaa atgttttctg ggcctcttct
      781 gaccgcgtgg cagtagacgc cgttggagtg tccctcctca agcacctagg aggaaacgag
      841 cagatcatga agcggaaaat cttcgagcag gagcagatcg cccgagccgt ggaactcggc
      901 ctgggagtct cctctccggg cgagattgaa ctggttgccg ccgatgccaa tagccgggaa
      961 tattgcgaac gggtggcggc ggtattgaat caaggctgat gactgggcca aatcccgagg
     1021 tcattcaatc cgcaatccgc cctgcactac gtagagtgca gggcagccct gaacctgata
     1081 tggttcaggg cgccttcctt tctttctccc tctgtcagcc ggattcttcc aaatcctgcg
     1141 gggcctgctg cggcctctac aactgggagg agcactcgcg gaagaccctg ctgtccatgc
     1201 tggagaagag gagcgctctt ttcctttctc tgggccggga tccggagatt ttccaggagg
     1261 tctgtgcccg cgaaatgttc cactccaacc caaagctcct ggaggaggtc tacaactgcg
     1321 aattcctagg atttctggat ggggggaaaa aaagggtagg gtgcctgctc catccttcgg
     1381 tgaacgaagg acaggacctt cgggatcatt gtttttacgg ggaggaaatc tgcgcagggc
     1441 atcattgtcc cagccactcc catctgaccc cggtggaacg gaaatccgtc gtcctggcat
     1501 tggaagactg gtatctctat gggctggtga tcaccgatat cgacctggtg aaagattttt
     1561 tccaccaggt gcagagccgc ctgggagaca gcgtaaggga agagcgaatg gaaaatttcc
     1621 aagttcggga ggctctgcgg gaattcttta ccctcaagga gtactggaag tttgcctctc
     1681 accggaaccg cttggggaaa tattatttct cctgctccga ataccaggtc gcccggatcg
     1741 aatacgagaa aaattggagg ataaaacctt cccggttcga taaaatcctg ataagcctgg
     1801 aatccgattt tcaaacccga gaggatattt tcgaggccga gtccatcatc gaaagaaaga
     1861 tcacaaggtt cttaagggcc tatgaggggt aggttcaagg gggtcaaaga ggacgaaaat
     1921 ccattggttt tcgaatatcc ctcgattata atggaatttg agagtctcct ttattcgatg
     1981 ttttctgtgc tctctgtggc taaaataatg caggaggaaa atgatgagtt ccaatactca
     2041 ggaaagaatc aaagctctgg agatcgccct gaataatgag gcccgggaaa aggattttta
     2101 cttgaaacac agcaagcgga cgaccaacaa tatgggtaaa gtaatgttcg ccaccatcgc
     2161 cagcgacgaa gacgaacatt accggagaat tctcggtctc cacaaacggc tgatccagga
     2221 aggaaagtgg ccggagacgg tgcccattga ggtgaagggg accgaggtca agaaggtcat
     2281 ccagaaagtg gtggatgccg taaacgctac tgcccgggcc gaccgggatg atatggaggc
     2341 agtgaagatc gccatcgact ttgaaaccaa gggggagaag ttttacggcg atctggccaa
     2401 aagcgtcgag aaccccatgg agaagaattt ctacgaattc ctggcttcaa tcgaacggga
     2461 gcaccgtctc tcccttcagg acactttgga atacttcgag gaccccgccg gctggttccg
     2521 gatcaaggaa agacttcacg tcgatggact ctgatcaaag gcggttgaag agctgattgg
     2581 ttgttggaaa aaccgagaac ccgttaacgg ggtttccgtt agcgggtttc ttttttgttc
     2641 ttttcggcgt tcctaggcgg aggaagaaaa gggagaggga gatgaggaaa gtgaaaaagt
     2701 ggatcgagtt gggccggact gttttccccc ctaacctctg gcttcctgcc ttttcgatcg
     2761 ttctgggggc cttgatgtgg gccatttcca tcaatggaat cctggtccat cataaactcc
     2821 tcagcggagg agtctccggt ttagccctgg tcgtctatta tctgattccc aagctttcta
     2881 tcggcctcat ggtctttctg atgaatattc ccaccttcat catgggctgg accatgatct
     2941 ccgggaaatt ctttgccttc agccttttgg gaatggcttc cttctctttt ttcctgaccg
     3001 ttaccacctg gctgcagatc gccgtggaaa acccgctttt ggcttgtctc ttcgccggcg
     3061 tcatctccgg tgtgggctcg ggcttgattt tccgcgccgg aggatcggga ggggggacgg
     3121 gaatcattgc catggtcttg aaccgcagat tttccgttcg cgtgggaatc gtctcctttt
     3181 tgctcaactc cgttcccttg gttctgggag ctttcctgat cgacctcaat tcggcccttt
     3241 actccattat ctatgtctat gtctcgggtt cggtcatgga ccggattatc accagtttca
     3301 acgaacgaag aagcgtctgg attatttctt cccaatcttc cgaggtctac ggacagattt
     3361 tgagcaaact tcgccggggg gcgaccctgc ttgccggaaa gggggcttat acccagtcgc
     3421 cggttgaggt catctacagc gtgatctctc cttttgaact cgctaagctg aaagacctgg
     3481 tcataaccat tgatcccaag gcatttataa ttattaatga aacccaagag gtaatcggaa
     3541 agggattcga tcaaccggga agcttctaat gactgaaaag cgcggtgcgg agtgaattcc
     3601 ggcccagatc gctggggggt aattcccttc taatttatgg gtgggtaaag cagaagcaca
     3661 tggacgaaaa gcgcagaaat aatctcctcc gacggggaaa gatgctccaa gggatccgtc
     3721 agtatttcct tggggaagat ttcgtggaag tcgaaacccc ttatctggta ccctcacccg
     3781 gaatggagcc gcacctagtg gccctggaag tgaatgtcca gttcccggat aaggaaaaga
     3841 ggaaaatgta cctgcataat tccccggagt attgcatgaa aaagctactg ggtcaggggt
     3901 gggataaggt ctttcaaatt tgccgggtct tccgggacga agaggtcagc cgaacccacc
//
LOCUS       DASPRR010000393         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_744123, whole genome shotgun sequence.
ACCESSION   DASPRR010000393 DASPRR010000000
VERSION     DASPRR010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744123"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1560)
                     /gene="cobA"
                     /locus_tag="VEL68_14450"
     CDS             complement(7..1560)
                     /gene="cobA"
                     /locus_tag="VEL68_14450"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXZ37218.1"
                     /translation="MSEGKVYLIGAGPGDPGLITVKGRDCLEKADVVVYDYLANEQLL
                     GYIREGAEKVYVGKKGGDHTLPQDQINDLIIAKAKEGKSVVRLKGGDPFIFGRGGEEA
                     EDLAQAGIPFEIVPGVTSAIAAPAYAGIPLTHRDYTSTVAFITGHEDPTKEESKIAWD
                     KISTGVGTLVFLMGVGNLTRIAQELMKNGRPPETPVALIRWGTLPEQETILGKLSNVG
                     EIAQSRKLKPPVIILVGEVVALRERLNWFETLPLFGKKVLVTRAREQASDLSERLRAL
                     GAIPVEFPTIGILPPESWTDADHCIQQLIRYDWIIFTSVNGVKFFLDRVLALGRDLRD
                     LKGPRICAIGPKTAEALEALKVRVEFVPQEYRAEAIFEGLKKEMLKDKSILLPRAKVA
                     RDVLPVELRKAGAVVDVVEVYQTILPKEDVTEVRELLKRREVSAITFTSSSTVGNFVE
                     MLGAEEARQLTAGIPVASIGPITAEKAKALGIKTAVMPAEYTIPALVRALVEYFKISD
                     QHSAFSGQP"
     gene            complement(1557..2498)
                     /gene="hemC"
                     /locus_tag="VEL68_14455"
     CDS             complement(1557..2498)
                     /gene="hemC"
                     /locus_tag="VEL68_14455"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXZ37219.1"
                     /translation="MKDRPKKIELRLGTRASLLAQQQAHMVKKRVEELNPEITVTLVH
                     IKTTGDKLDFPLLKVGGKGLFVKEIEEALTRKEIDLAVHSAKDLPAIIPEGLILMAFP
                     EREDPRDALVSQGGKRFEEVPQGGKIGTGSLRRKAQLLNLRPDLEIVPLRGNLDTRLK
                     KLMAMNLDGVILAAAGLRRMNWEGQVSEFFDPETMVPAIGQGALAVEAREGDERVRRA
                     VASLDHPPTRVSVLAERAFLRRLEGGCQVPIGGLARIHVEKLALTGLVAGVDGRRVVK
                     GKVEGPLEKNTELGVQLAEDLLGRGAEEILREVYQKG"
     gene            complement(2506..3168)
                     /locus_tag="VEL68_14460"
     CDS             complement(2506..3168)
                     /locus_tag="VEL68_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HXZ37220.1"
                     /translation="MRYFPVFLDLQDKSCVVVGGGRVAERKVKTLLKAGARVKVISPK
                     LTGPLARLESEERIVQYPRLFRRKDLRGAFLTIAATDDRAVNQRVFQEATENRIPVNV
                     VDDPAHSSFIVPSLVKKKDLVLAISTSGRSPALARLLRQKLEKEIGPEYGRFLRLLGA
                     VRRKMIPLGLGQRWNQKIFRKLVREDLLSLIKGKKFRELNHRLAKILGPGFSLEELGL
                     RW"
     gene            complement(3165..>3588)
                     /gene="hisH"
                     /locus_tag="VEL68_14465"
     CDS             complement(3165..>3588)
                     /gene="hisH"
                     /locus_tag="VEL68_14465"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXZ37221.1"
                     /translation="KPFLGICLGLQLLFSESEGFSSYKGLGVFEGKVIRFPFSLPGVP
                     PARDSLKVPHMGWNSVQIKRKPPALERIQEGTYFYFVHSFFPAPDDPGIITTTTDYGG
                     EFVSSVAKGNLFACQFHPEKSQSAGLQILQNFGRLAQP"
BASE COUNT          730 a         1083 c          926 g          849 t
ORIGIN      
        1 ctccttctag ggctgaccgc tgaaagctga atgctgatcg cttattttga agtattccac
       61 caaggcccgg accaatgcgg gaatcgtgta ttcagccggc atgactgcgg tcttgatccc
      121 cagggctttt gccttctcag cggtaatggg gccgatggaa gccaccggaa ttccggcggt
      181 cagctgccgg gcttcctccg ctcccagcat ctccacaaag tttccgactg tggaagaact
      241 ggtaaaggta atggccgaga cctctcttct ctttagtaac tcacggacct cggtgacatc
      301 ctctttggga aggatggtct ggtaaacctc cactacatcc accaccgccc cggctttcct
      361 taactccacc ggaaggacat cccgggccac cttggctcgg gggagcagaa tacttttgtc
      421 ctttagcatt tctttcttga ggccttcaaa aatcgcctcg gcccggtatt cttggggcac
      481 aaattctact cggaccttca aggcttccag ggcttcagcg gtcttggggc cgatggcaca
      541 aatccgcggg ccttttaaat ccctcaggtc ccggccgaga gccaagaccc gatccagaaa
      601 gaatttcacc ccattaacac tggtgaaaat aatccaatca taccggatca gctgctggat
      661 gcaatgatcg gcgtcagtcc agctttcggg gggaagaatc ccgatggtgg gaaactcgac
      721 gggaatggct cccagggccc gtaatctctc ggacaagtcg ctggcctgct cccgagcccg
      781 ggtcaccagg acttttttcc cgaaaagagg aagcgtctcg aaccagttca gtctctcccg
      841 tagggcaaca acctcgccca ccaggatgat gaccggaggt ttaagtttgc gggattgggc
      901 gatctctcca acgttggaaa gcttccccag aatggtttcc tgttcgggta gggttcccca
      961 gcggatcaag gccacgggag tctccggggg gcggccgttc ttcatcaatt cctgggcgat
     1021 gcgggtcagg tttcccaccc ccatgagaaa aaccagcgtt ccaacccccg tggaaatttt
     1081 gtcccaggct atcttcgact cctccttggt cgggtcctcg tggccggtaa taaatgccac
     1141 agtagaagtg tagtcgcggt gggtcaaggg aatgccggcg taggccggcg cagcgatggc
     1201 tgaggtgact ccggggacga tctcaaaggg aatcccggcc tgggccaaat cctcagcctc
     1261 ctctcctccc cggccgaaaa taaaggggtc ccctcctttc aggcgcacca ccgacttccc
     1321 ttccttggcc ttggcgatga ttaaatcatt gatctgatct tgcgggaggg tgtggtcgcc
     1381 ccctttcttt ccaacgtaga ccttttccgc cccttctctg atgtacccta gcaattgttc
     1441 attggccaga tagtcgtaaa cgaccacatc cgccttttcc aggcagtcac gccccttgac
     1501 ggtgatcagt ccggggtctc cgggccctgc tccgatgagg tagacttttc cctcgctcat
     1561 cctttttggt agacctctcg taaaatttct tcggctcctc ttcccagcag gtcctcagcc
     1621 agctgaacgc ccaattccgt atttttctcc agaggccctt ccactttccc ctttaccact
     1681 ctcctgccgt ccactccggc aaccagaccg gttagagcaa gcttctctac atggattctg
     1741 gccaagcctc cgatggggac ctggcatccc ccttccagtc tccggagaaa ggctcgctcc
     1801 gccagaaccg aaacccgggt cggagggtgg tccagggaag ccacggctcg acggactcgc
     1861 tcatccccct ctcgggcttc aacggccagt gccccttgtc caatcgccgg gaccatcgtt
     1921 tcaggatcga agaattccga gacctgcccc tcccagttca tcctgcgcag ccctgcagcg
     1981 gccaaaatca ccccatccag attcatggcc atgagctttt tgagacgggt gtccaggttt
     2041 cctcgaagag ggacgatctc caggtccggc ctcaggttta gcaactgagc ctttctccta
     2101 aggctacccg ttcctatttt cccaccttgg gggacctctt caaacctctt tcctccctgg
     2161 gataccaagg cgtcccgggg gtcctccctc tcgggaaagg ccatcaggat aaggccttcg
     2221 ggaattatgg ccggcaggtc cttggcgctg tgcaccgcca ggtcgatttc cttccgggtc
     2281 aaggcctctt cgatctcctt gacaaataac ccttttcctc caaccttaag aaggggaaag
     2341 tcaagcttgt cgccggtggt cttgatatgg accagggtga cggtaatttc gggattcagc
     2401 tcttctaccc tttttttcac catatgggcc tgttgctgtg ccagaaggct cgcccgggtg
     2461 cccaggcgaa gttcaatttt ctttggccgg tctttcatct gtccttcacc acctcaggcc
     2521 cagctcctcc agggaaaatc ccggccctag gatcttagcc agtcggtgat ttagttcccg
     2581 aaatttcttc cctttgatca gagaaagcag gtcctctctc accaatttcc gaaagatttt
     2641 ctggttccat cgctgcccca ggcctaaggg aatcattttc ctccgcactg cccccagcaa
     2701 ccttagaaac cggccatatt cggggccgat ctccttttcc agcttttggc gaagcaggcg
     2761 cgccagggcc gggcttcgcc cggatgtaga tatcgccaaa accaagtctt tctttttcac
     2821 caaagaggga acgatgaagc tgctgtgagc cgggtcatcc accacgttaa ccgggatgcg
     2881 attttccgtc gcttcttgaa aaaccctttg gttgacagcc cggtcatcgg ttgccgcgat
     2941 ggttaagaaa gcgccccgca ggtccttccg ccgaaataag cggggatatt ggacgattct
     3001 ctcctcgctc tccagtcgag ctaagggtcc ggttaatttg ggactgatca ctttgacccg
     3061 ggcgcctgct ttcaggaggg tcttgacttt gcgctccgcc acccgacctc cgccgacaac
     3121 gacgcaggat ttatcctgga ggtccaaaaa aacggggaag tatctcatgg ctgtgcgagg
     3181 cgtccgaaat tctgcaggat ttggagaccg gcggactggc tcttttccgg gtgaaactgg
     3241 caggcaaaga gattgccttt tgccacgctg gacacgaatt ctccaccata atctgtggtg
     3301 gtggtgatga tcccaggatc atcgggagcc gggaaaaacg agtggacgaa gtaaaaatac
     3361 gttccttcct gaattctctc cagggctggg ggttttcttt tgatctgaac tgaattccag
     3421 cccatatggg gaaccttgag ggaatccctg gccggaggga cccctggaag ggaaaagggg
     3481 aagcggatta cttttccctc aaaaaccccc aggcccttgt acgaagagaa tccctcactt
     3541 tcggaaaaaa gaagctgcag gccaaggcag attcccagaa agggcttt
//
LOCUS       DASPRR010000394         7834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2186418, whole genome shotgun sequence.
ACCESSION   DASPRR010000394 DASPRR010000000
VERSION     DASPRR010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186418"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VEL68_14470"
     CDS             <1..346
                     /locus_tag="VEL68_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXZ37222.1"
                     /translation="KANEVPEAIRKGIEQAKKGLIQVPLNQGTIPYEVVGRFGAARIL
                     LKPASPGTGVIAGGAVRAVIESAGIENILTKCLGSRNPHNVIKATVEGLRTLKNPDEI
                     ATKRGKAVAEIG"
     gene            452..637
                     /gene="rpmD"
                     /locus_tag="VEL68_14475"
     CDS             452..637
                     /gene="rpmD"
                     /locus_tag="VEL68_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009791311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXZ37223.1"
                     /translation="MEKKMIEVTLVRSGVNRPETQQRTLRGLGLTRMNRTVRLNDTEA
                     IRGMVRKVIHLVKVVEK"
     gene            647..1087
                     /gene="rplO"
                     /locus_tag="VEL68_14480"
     CDS             647..1087
                     /gene="rplO"
                     /locus_tag="VEL68_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXZ37224.1"
                     /translation="MKLSELRPPEGARKGKKRIGRGEGSGHGGTSTKGHKGYKARSGG
                     KRSPGFEGGQMPLQRRLPKRGFKNPFRKQWSVINLRDLSAFPEGAVVDVESLKSKGLV
                     KKIGSGVKILGEGEVSRPLTVRAQAFSLSAKKKIEAAGGKAEVI"
     gene            1091..2398
                     /gene="secY"
                     /locus_tag="VEL68_14485"
     CDS             1091..2398
                     /gene="secY"
                     /locus_tag="VEL68_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXZ37225.1"
                     /translation="MFQGFQNIPKIPELKRRIFISLLLLAVYRIGVHVPTPGIDASAL
                     AAFFAQAKGTLFGFIDMFSGGAFERLSVFALGIMPYISSSIILQLLTVVVPHLERLSK
                     EGEAGRKKITQYTRYGTVLLSLIQGFGISVGLERMTGPAGEAVVLDPGWSFRLMTMIS
                     LTAGTAFIMWLGEQITERGIGNGISLIIFAGIVARMPTAISNSIRLLRTGEIGILITL
                     FLVALMVGVVGAIIYMERGQRRIPVQYAKRIVGRRMYGGQSTHLPLKINTAGVIPPIF
                     ASSIIMFPATIANFIDHPWMKAVSNQLAPGSVLYELIYVGFIFFFCYFYTAVTFNPTD
                     VADNMRKYGGYIPGIRPGKKTAEFVDRVLTRITFTGALYVSAVCVLPSILIARFNVPF
                     YFGGTALLIVVGVALDTVGQIESHMLMRHYEGFMKQGRIKGRR"
     gene            2408..3184
                     /gene="map"
                     /locus_tag="VEL68_14490"
     CDS             2408..3184
                     /gene="map"
                     /locus_tag="VEL68_14490"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXZ37226.1"
                     /translation="MGAYPLPLEERIILKSPQEIERMRSSNRIVAEILQKVAAAVRPG
                     VKTRELDALAEDLLRRYTARSAFKGYNGYPAVLCTSVNEEVVHGIPSERVLQEGDILS
                     LDFGAVWDDYYGDAAVTVPVGAISEEARRLLRVTEEALGKAIEQARPGNHVGDISAAI
                     QGHVESRGFSAVRDFVGHGIGKFMHERPQIPNFGLPGRGVRLKPGMILAIEPMINAGG
                     YAVEVLEDGWTAVTRDRSLSAHFEHSVAVTENGPYILSQL"
     gene            3240..3458
                     /gene="infA"
                     /locus_tag="VEL68_14495"
     CDS             3240..3458
                     /gene="infA"
                     /locus_tag="VEL68_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXZ37227.1"
                     /translation="MPKEDAIEVTGTVIETLPNAMFRVELETGHRVLAHISGKMRLHF
                     IKILPGDKVTVELSPYDLNRGRIIYREK"
     gene            3489..3602
                     /gene="rpmJ"
                     /locus_tag="VEL68_14500"
     CDS             3489..3602
                     /gene="rpmJ"
                     /locus_tag="VEL68_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXZ37228.1"
                     /translation="MKVKASVRRICDKCKIVKRKGVVRVICPNTRHKQRQG"
     gene            3704..4075
                     /gene="rpsM"
                     /locus_tag="VEL68_14505"
     CDS             3704..4075
                     /gene="rpsM"
                     /locus_tag="VEL68_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXZ37229.1"
                     /translation="MARIAGIDLPKNKNVEIALTYIHGIGRTTARKVLQMAQVDLAKK
                     TDTLSDAEVAKIREAIDRSNLKIEGDLRREVSMNIKRLMDLGTYRGLRHRRSLPVRGQ
                     RTHTNSRTRKGPRRQIGGKRK"
     gene            4088..4480
                     /gene="rpsK"
                     /locus_tag="VEL68_14510"
     CDS             4088..4480
                     /gene="rpsK"
                     /locus_tag="VEL68_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXZ37230.1"
                     /translation="MSPGKPSGAKKKERRNIQNGVAHIQSTFNNTIVTITDVSGNVIA
                     WASAGSAGFKGSRKSTPFAAQLAAEQAAKKAMEQGVRSIEVYVKGPGAGREAALRSLQ
                     AAGFRVDLIRDVTPIPHNGCRAPKRRRV"
     gene            4513..5139
                     /gene="rpsD"
                     /locus_tag="VEL68_14515"
     CDS             4513..5139
                     /gene="rpsD"
                     /locus_tag="VEL68_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXZ37231.1"
                     /translation="MARYNQSVCRLCRRENLKLYLKGERCYSDKCAVDRRSYPPGQHG
                     QGRVKFSNYALQLREKQRVKRMYGVLEKQFRNFFERAERMKGITGTNLLLLLESRLDN
                     LVYRLGFANSRNEARQLVRHSHFLVDRKKVSIPSYVVKVGGVIELREKSRKIARISEA
                     LESVARRGVPSWLELEKDHFRGRLVSLPAREDLTMPIKEQLIVELYSK"
     gene            5182..6198
                     /locus_tag="VEL68_14520"
     CDS             5182..6198
                     /locus_tag="VEL68_14520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXZ37232.1"
                     /translation="MYRNWETLIKPKPLEVEQESLTPTYGRFHVEPLERGFGITIGNS
                     LRRILLSSLQGAAITAVRIKGVLHEFSTIYGVREDVTDVILNIKELLVKLHTDGPEIL
                     RLKINKPGVVKAQDIQPNPNVGILNPDHLIATLSEDGELDIEMVVKRGRGYMPAERNR
                     EEGDPIGTIPIDALFSPIRRVNYTVTHSRVGQITDYDRLTLEVWTNGNVTPGDALGYA
                     AKILKEQIAVFITFDEQEALPRAPEVQEPGKLNDNLFKSVDELELSVRSANCLKNADI
                     RYIGDLVQKTEVEMLKTKNFGRKSLNEIKEILSEMGLSLGMKLENWPPAELGPKTIEG
                     LGKE"
     gene            6203..6655
                     /gene="rplQ"
                     /locus_tag="VEL68_14525"
     CDS             6203..6655
                     /gene="rplQ"
                     /locus_tag="VEL68_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXZ37233.1"
                     /translation="MRHRVAGRKLGRTTSHRVAMLRNLVTSLLEHERVKTTDAKAKEV
                     RPLAEKLIGLAKRGDLHARRQALSVVRKPDLVKKLFETLSPRYQSRTGGYLRIVKVGY
                     RPGDGAPVSLVELIGEGKEGTKKAKAKKKREKGKRAERTKLPEAASKP"
     gene            6717..7544
                     /locus_tag="VEL68_14530"
     CDS             6717..7544
                     /locus_tag="VEL68_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ37234.1"
                     /translation="MIDLREVTFSYPTSTGASKPVFEGLTLSIPRGRYVFLMGPNGSG
                     KSTLGKLVKGILSPSSGQIRIAGQLLKPGEISPRIGYLFSNPENQIISSVVEEDVAFG
                     PGNRGMDPALLSLRVDESLRLVGMEGYRTHAPHLLSGGQQQKIVIAGILAMECEALVL
                     DEPTAMVDQEARQEILDLFLKLHRERNITFLHITHSLEEALWSKDLIYLEEGRILYSG
                     PPEEFLASPRFSESSSIQIPPLLRLVQGLRQRGHDIPSGIRSYEQLKRFLLQGDHRV"
BASE COUNT         1805 a         2137 c         2389 g         1503 t
ORIGIN      
        1 aaaagccaac gaggttcccg aagccattcg caagggaatc gagcaggcca agaagggcct
       61 gatccaggtt cccttgaacc aggggacgat cccctacgag gtggtggggc ggttcggggc
      121 agcgcggatt ctcctcaaac cggcgtcccc gggtactggg gttattgccg gaggagcggt
      181 gcgggcggtc atcgaatcgg cgggaatcga aaacatcctc accaagtgcc tgggctcccg
      241 caatccccac aatgtgatca aggccactgt ggaggggctc aggaccttga agaatcccga
      301 tgaaattgcc accaagaggg ggaaggctgt agccgaaatc ggctaagggg tttcttttcg
      361 gacgcaggtg aacgcagatg ggattaagaa aatttattcc ggttttatct gcgccattct
      421 gccccccatt cataagagtc agtagggaac catggaaaaa aagatgattg aagtcaccct
      481 ggtacgaagc ggggtcaacc ggcccgaaac ccagcagcgg acccttcgcg ggctgggcct
      541 gacccggatg aaccggaccg tccggcttaa cgatacggag gccattcggg ggatggtgcg
      601 caaagtgatc catttggtaa aagtcgtcga aaaataggat gggacgatga aactgagcga
      661 actgaggccc ccggaggggg cccgaaaagg aaaaaagagg atcggtcggg gagagggatc
      721 ggggcacggc gggacctcca ccaaagggca taaggggtac aaggcccgtt cgggggggaa
      781 gcgatccccc ggttttgagg gaggacagat gcccctccag cgccggcttc ccaagagggg
      841 gttcaagaac cctttccgca agcagtggag tgtgatcaac ctgcgagacc tttctgcctt
      901 ccccgaaggg gccgtggtgg atgtggagag tttgaagtcc aagggattgg tgaagaagat
      961 cgggtccggg gtgaagatcc tgggggaagg ggaggtctca cgcccgttaa ccgttcgagc
     1021 ccaggccttc agtctttcgg ccaaaaaaaa gattgaggcg gcagggggaa aagcggaggt
     1081 catctaaacc ttgttccagg gattccagaa tatacccaag attccggagc tgaagaggcg
     1141 gattttcatc agcctcctgc tcttggccgt ctaccgcatc ggggttcacg ttcccacgcc
     1201 ggggatcgat gcttcggctc tggcggcctt tttcgcccag gccaaaggaa ccctatttgg
     1261 tttcattgat atgttttccg gcggggcttt tgagcggctg tcggtcttcg ccctgggaat
     1321 tatgccctac atcagttcct ccatcatcct tcagcttctg accgtggtgg ttccccatct
     1381 ggaaaggctc tccaaggaag gggaggcggg gcgaaagaag atcacccagt atacccgcta
     1441 tggaacggtg ctgctgagcc tcatccaggg attcgggatc agcgtggggc tggagcggat
     1501 gaccgggccg gctggagaag ctgtggtctt ggatcccggg tggtccttcc gtttgatgac
     1561 catgatcagc ctaaccgcgg gaacggcctt cattatgtgg ctgggggaac agatcaccga
     1621 aaggggcatc ggcaacggga tctcgctgat catcttcgcc ggaatcgtgg cccgaatgcc
     1681 tactgccatc agtaacagta tccggctcct gaggacggga gagatcggaa ttctgattac
     1741 cctcttcttg gtagccctca tggtgggcgt agtgggagcc atcatctaca tggaacgggg
     1801 gcagcggcga attccagtgc agtacgccaa gcgaatcgtg gggcggagga tgtacggcgg
     1861 gcagagcacc catctgcccc tgaagatcaa caccgccgga gtcatcccgc ccatcttcgc
     1921 ctcctcgatc atcatgtttc cggcgaccat cgcgaatttc atcgatcatc cctggatgaa
     1981 ggccgtctcc aaccagctcg ccccgggttc ggtgctgtac gagctcatct acgtgggttt
     2041 catcttcttc ttctgctatt tttacaccgc ggtcaccttc aatcccacgg acgtggcgga
     2101 taacatgcgt aaatacgggg gttatatccc gggaatccgc cccggaaaga agaccgccga
     2161 gttcgtggac cgggtcctga cccgtatcac cttcaccgga gccctttacg tgtccgcggt
     2221 ttgcgttctg ccttcgatcc tgatcgcgag gttcaatgtt cctttctact tcgggggtac
     2281 ggctctgttg atcgtcgtgg gcgtagccct ggatacggtg gggcagatcg agtcccatat
     2341 gctcatgcgc cactatgaag gattcatgaa gcagggacgg atcaaaggga ggagataggg
     2401 aaagacgatg ggggcttatc ctctccccct ggaggaacgg atcatcctga agtctccgca
     2461 ggaaatcgaa agaatgcgga gcagcaaccg gattgtggcc gagattctgc agaaggtggc
     2521 tgccgcggtc cggcccgggg tgaaaacccg ggaattggat gccctggccg aggaccttct
     2581 gcgcagatac acggcccgat cggctttcaa ggggtacaac gggtatccgg cggttctttg
     2641 cacttcggtg aatgaggaag tggtccacgg aatcccctcg gaacgcgtgc tccaggaggg
     2701 ggacattttg agcctcgatt tcggggccgt ctgggatgac tactacgggg atgcagcagt
     2761 taccgtgccc gtgggagcga tctccgagga ggcccggcgg ctgctgcggg tgaccgagga
     2821 ggctctgggg aaggcaattg aacaggcccg cccgggaaat cacgtgggcg atatttccgc
     2881 ggccatccaa ggccatgtgg aaagtcgggg attttccgcg gtgcgcgatt tcgtgggaca
     2941 tgggatcggc aagttcatgc acgagaggcc ccaaattccc aatttcgggt tacccgggcg
     3001 gggggtccgc ctgaaacccg ggatgatcct ggccatcgag cccatgatca acgccggggg
     3061 ttacgcggtg gaagtcctgg aggacggctg gaccgcggtc acccgagacc gcagtctttc
     3121 ggcccatttc gagcattcgg tggcggtgac cgagaacgga ccctacattt tgagtcaact
     3181 ctgaggccca cgggacggaa agagcggccc ctggcccgat tggaatcgac aggtttccaa
     3241 tgcccaaaga agatgccatt gaagtcacgg gaacggtcat agaaacactt cccaacgcca
     3301 tgttccgggt agaactggag accggccacc gggtcttggc ccatatttcg ggcaagatgc
     3361 gcctgcattt catcaaaatc ctcccggggg ataaagtgac cgtggaactc tctccttacg
     3421 atttgaatcg aggacggatc atctatcggg aaaaataggc cccgcttccg ggggcagagg
     3481 gaagcaacat gaaagtcaaa gcctcagtac ggcgaatctg tgacaagtgc aaaatcgtaa
     3541 agagaaaagg agtggtccgg gtgatctgcc cgaatacccg gcacaagcaa agacaaggat
     3601 aaagacagct ttcagcggtc agcgatcagc aaaggcaaaa tatgatttgg tttagctgaa
     3661 cgctgaaagc tggtagctga cagcttattt caaggaggtt ggggtggcac gcattgccgg
     3721 aatcgatctt cccaagaaca aaaacgtcga aatcgcgctg acttacattc atggaatcgg
     3781 acggaccacg gcccgcaagg ttttgcagat ggctcaggtg gacttggcca agaaaaccga
     3841 caccctgagc gacgccgaag tggccaagat ccgggaagcc atcgatcgga gcaacctgaa
     3901 gattgagggg gacctgcggc gggaagtgtc catgaatatc aagcgcctta tggacctcgg
     3961 tacctaccgg gggctgcgcc accgccgttc cctgccggtg agaggccagc gcacccacac
     4021 caactctcgg acccgcaaag ggccccgtcg tcagatcggc gggaagagga aatagtcagg
     4081 aggaagaatg tcgccaggaa agccatcggg ggccaagaag aaagagcgcc ggaatatcca
     4141 gaacggcgtc gcccacatcc agtctacgtt caataatacc atcgtaacca tcaccgatgt
     4201 cagcggaaat gtgatcgcct gggccagcgc gggcagcgcg gggttcaagg ggtcgcgcaa
     4261 gagcactccc tttgccgccc agctggccgc cgagcaggcg gcgaaaaaag ccatggagca
     4321 aggagtccgg tccattgaag tctacgtcaa agggccggga gccgggcggg aggcggcgct
     4381 ccgttccctc caggcggctg gctttcgagt ggacctgatc cgggatgtga ccccgattcc
     4441 ccacaacggc tgtcgtgccc ccaagaggcg gcgggtttga agtccgtccc gggctgagag
     4501 aggaggtttg gattggccag gtacaaccaa tcggtctgcc ggctctgccg gcgggagaat
     4561 ttgaagctct accttaaagg ggagaggtgt tattccgata aatgcgccgt cgaccggagg
     4621 agctatccgc cgggacagca tggccaggga agggtgaagt tctccaacta tgccctgcag
     4681 ctgcgggaga agcaaagggt caagcgcatg tacggggtgc tggagaagca gttccggaac
     4741 ttcttcgaga gggctgaaag gatgaagggg atcaccggga ccaacctcct cctcctcctg
     4801 gaaagtcgtc tggacaacct ggtctatcgg ctgggatttg ccaactccag gaacgaagcc
     4861 cggcagctcg tccggcacag ccatttcctg gtggaccgca agaaagtgag tattccttcc
     4921 tatgtggtga aggtgggagg ggtgatcgaa cttcgggaga agagtcggaa gattgcccga
     4981 atcagcgaag ccctggagag cgtggcccgg cggggggtgc cgtcctggct ggagctggag
     5041 aaggaccatt tccggggacg gctggtctcg cttcctgccc gggaagacct gaccatgccc
     5101 atcaaagagc agttgatcgt cgaactgtat tccaaataaa accttcgatg agcccgctcc
     5161 gagcgggatc gaggagacgc tatgtatcga aactgggaaa ctctaatcaa accaaagccc
     5221 ttggaggtcg agcaggagag tttgacccca acctacggcc gctttcatgt ggagcccctg
     5281 gagcgcggat tcgggatcac gatcgggaac tccctgcgac gcatccttct gtcttccctc
     5341 cagggggcgg cgattaccgc agtccggatc aagggagttc tccatgagtt ttccaccatt
     5401 tacggggtcc gcgaggacgt aaccgacgtc atcctgaaca tcaaggaact tctggtgaaa
     5461 ctccataccg acggcccgga aatcctgcgg ttgaagataa acaaaccggg ggtggtgaaa
     5521 gcccaggaca ttcagcccaa ccccaacgtg ggaattttga accccgatca cctgatcgcc
     5581 accctctcag aggacggaga gctcgacatc gagatggtgg taaagcgggg gagggggtac
     5641 atgccggcgg agcgaaaccg ggaggaaggg gaccccatcg gaacgattcc tatcgacgca
     5701 ctattctccc ccattcgcag ggttaactac acggtgaccc attcccgggt cggacagatt
     5761 accgattatg accgcctcac cctggaggtc tggaccaacg gaaatgtgac tccgggcgat
     5821 gccctgggct atgcggccaa gattctgaag gaacagatcg cggtcttcat caccttcgat
     5881 gaacaggagg cccttcccag agcccccgaa gttcaggagc cgggaaagct caacgacaac
     5941 ctgttcaaga gcgtagatga actcgagcta tcggtccgtt cggccaactg tttgaagaac
     6001 gccgacattc gttatatcgg cgacctagtg caaaaaaccg aagtggagat gctgaaaacc
     6061 aagaacttcg ggcgcaagtc gctgaacgag atcaaagaga tcctgtccga gatggggctg
     6121 tccctgggga tgaagctgga gaactggccc cccgccgaac tgggaccaaa gaccatcgaa
     6181 ggtttaggga aggaatagca acatgcgtca tcgagtcgcg ggaagaaagc tgggtcggac
     6241 cacgagtcac cgggtagcca tgctccggaa tttggtcact tcgctcctgg agcatgaaag
     6301 ggtgaagacc accgatgcca aagctaagga agtccggccc ctggcggaaa aactgatcgg
     6361 cctggccaag cggggcgacc ttcacgcccg gcgtcaggcc ctctccgtgg ttcggaagcc
     6421 tgatctggtc aagaaattat tcgaaaccct ctccccccgt taccagtctc gaaccggcgg
     6481 ataccttcga atcgtgaagg tggggtaccg tccgggggac ggagcgccgg tttccctggt
     6541 ggaattgatc ggcgagggca aggaggggac caaaaaggcc aaagccaaga aaaagcggga
     6601 gaaaggcaaa cgcgccgaaa ggaccaagct cccggaagcc gcttcgaaac cgtgaagcag
     6661 gatcgggata ttaaaaaggg cgaggccgct ggcctggccc ttttttttcg ggcgagatga
     6721 tcgatctcag agaggtgacc ttttcctatc ctacttccac cggggcgtcc aagccggtct
     6781 ttgagggatt gaccctctcc atcccccggg gtcgatatgt ttttctcatg ggtcccaacg
     6841 gctcggggaa gagcactctg gggaagctgg tcaaggggat tctctctccc tcttctggcc
     6901 aaatccggat cgccgggcaa ctcctgaaac ccggcgaaat ctctccccgc atcggctacc
     6961 tattttccaa ccccgaaaat cagattatct cctccgtagt ggaagaagac gtggccttcg
     7021 gcccggggaa ccgggggatg gaccctgccc tcctgtccct tcgggtggat gagagcctgc
     7081 gcctggtggg aatggaaggc taccgcaccc acgctcccca tctcctatcc ggcggacagc
     7141 agcagaaaat cgtcatcgcc ggcatcctgg ccatggagtg cgaagcgctc gtcctggatg
     7201 aacctacggc catggtggac caggaagccc gccaggaaat ccttgacctc ttcttgaagc
     7261 tccaccggga gaggaacatc accttcctcc atatcaccca ttctctggag gaggcccttt
     7321 ggtctaagga cctgatctac ttggaagagg ggcggattct ttattccggg cccccggaag
     7381 agttcctggc ctcccctcga ttctccgaat cctcctccat tcaaatcccc cccctcctcc
     7441 gcctggtcca aggtttgcgg cagcggggcc acgacattcc ttcgggaatt cgttcctacg
     7501 agcaattaaa gcggttcctc ctgcaggggg atcacagagt ttgaaacttc caggatgttt
     7561 ggctttggtc cgcggggcca aaccaactat atcctgctct gttcactctt cagcaaataa
     7621 tagaagagtc gaaaaatcca aaatccgcaa ttggaaatcc aaaattcaat gatctttgct
     7681 ttttacgcgc aacggccttg cttccgggtc acaaactagc ttttgcactg aaaggaagaa
     7741 aacaaaaggg aagagggggc acccacttta tgccttgaca ctcaggaaag cgaaaaccta
     7801 taattttttt atcaaggctc ccaagcgatc agaa
//
LOCUS       DASPRR010000395         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_409000, whole genome shotgun sequence.
ACCESSION   DASPRR010000395 DASPRR010000000
VERSION     DASPRR010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409000"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VEL68_14535"
     CDS             <1..1344
                     /locus_tag="VEL68_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37235.1"
                     /translation="LRMGLSCPYGCLQGPCRIDPFGRGPERGLCGLDREGMVAALLLR
                     LSLSGALEAMPTPAREISWDPALKDAVSRVSKKLGGEQISSTEIQTAASCLIRPMESP
                     EGLINQSLRLALLTIGWLAKGAASQGRKKSFPLAVGYGLLAQKDFNIGICGQPSPEFL
                     KVVLAQVNRSLSGGGQLVSLGEFIPLDGSYLPCVCTSGESELVLSSGQIKLLIAGPGT
                     DPSILELCRALNIPSLSSPDPKSVATLVAKAGKSGGAPAQSPFSPQPSLVEEAEAAGS
                     VSLLEESFKEMPAKKLALLGGADHPYHSLGWIPTEVTPALQAKGFAVSSWGDAALWMV
                     KKGLASQKMKSPIQILGGEQGAILALKPLAACGRLKDVKGVCFTGMKSCQDLALALGL
                     ASMGLKVSVAVPLPLWGSEKVRNLLQEKLAALGGSLTHYDHPAQAEEILDWFLKN"
     gene            1427..2695
                     /locus_tag="VEL68_14540"
     CDS             1427..2695
                     /locus_tag="VEL68_14540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HXZ37236.1"
                     /translation="MDIVIVGSGPAGVHAALQCRQSWPRKSVTLIEGEKSIGTCRPML
                     PQFMAGQVKGEKLYLFRPAEDPLLVLKTGTKVRSLDKEGKTVQLENGETTAYERLILA
                     PGGRPIIPRIEGIDGLEGLFPVRNLPEAEKVHNWITKDQKIVVLGGGLVGVKTAVYLR
                     TAGFQIAIVEKEDHLLPQALRAETAKYVEEHFRRMGIDLYLGQTLESAEGEYGKIKRA
                     RAGGQWIPCHALLLAIGSVPNISFLEGSGLLEEGRLRVSAALQTKDPRILAAGDAVTI
                     FTPDGKSYTPWTWPQAVSQGKLAAANLYRSNPLPLKALTRVNSMNLQGLALNMLGAFV
                     EGSEEICYAPPLEGILRQVFLREGRITGGALVGDITGAGPLHHLIIQSQDSISEVRRL
                     IKPSFRLMSPNLSERGTGRRRARLIPGEER"
     gene            2698..3324
                     /locus_tag="VEL68_14545"
     CDS             2698..3324
                     /locus_tag="VEL68_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ37237.1"
                     /translation="MLIRKDLCVGCGRCQPYCPVGAIHFEELKSIVDQEVCYECGTCL
                     RSEICPVDAIEESPYVYEYPRALRKFFSDPNATHALTGIAGRGTEESKSNDVINRVVP
                     GEVGMAVEFGRPTLGMDLTNIQKVTRAMAKAGFTKIEPENPFNDMIQNYESGDLKPEL
                     MGEKVLSAIMEFKITPDRLGLALRTIKEAAKRWTASSWWMSTPGWSPG"
BASE COUNT          739 a          967 c         1027 g          663 t
ORIGIN      
        1 ctacggatgg ggctgagctg cccttacggt tgcctccagg ggccctgccg catcgacccc
       61 tttggccgcg gaccggaacg ggggctctgc gggctcgacc gggagggcat ggttgccgcg
      121 cttctgctgc gactgagcct ctccggggcc ctggaagcca tgcccacccc cgccagggag
      181 atttcctggg atcccgcttt gaaagatgcg gtctcgcggg tctcgaagaa gcttggcggg
      241 gaacagatct cttcgacgga gattcaaacc gccgcttcct gtttaatcag gcccatggaa
      301 tcccccgaag gtctgatcaa ccagtccttg cggttggccc tgctgacgat cggctggctc
      361 gccaagggag cagcgtccca gggacggaaa aaatcgtttc cccttgccgt cggctatggt
      421 cttttggccc aaaaggattt caacatcggg atttgcggac agccgtctcc tgaattcctg
      481 aaagtggtcc tggcccaggt caaccgaagt ctttccgggg gcgggcagct tgtctccctg
      541 ggcgaattca tccccctgga tggaagctat ctgccctgcg tatgcacttc gggggaatcc
      601 gagctggtat tgagttcggg ccaaatcaag ctcctgattg ccggaccggg taccgatccc
      661 tccatcctcg agctttgccg ggccctgaac attccttccc tttcatcgcc ggaccccaaa
      721 tcggtggcga ctctggtggc taaggccgga aagagcggag gggctccggc tcaatcccct
      781 ttcagccctc agccttcttt ggtggaggaa gccgaagcgg ccggcagtgt atcgcttctg
      841 gaggaatcct tcaaggagat gccggcgaaa aaactggccc ttctgggcgg agccgatcat
      901 ccctaccact ccctgggttg gatcccgacg gaagtaaccc ccgcccttca ggccaaggga
      961 ttcgcggttt cgagttgggg cgatgccgcg ttatggatgg tcaaaaaggg ccttgcctct
     1021 caaaaaatga aatcccccat ccagattctc ggcggtgagc agggggcgat tctggcgctg
     1081 aaacccctcg cggcctgcgg acggctgaag gacgtaaaag gggtatgttt taccggaatg
     1141 aaaagctgcc aggacctggc actagccctc gggctggcca gcatgggcct aaaggtcagc
     1201 gtggccgtac cgctgcccct ctggggaagc gaaaaggtcc gcaatctcct ccaggagaaa
     1261 ctggccgctc tgggtggaag tctgactcac tatgaccatc cggcccaggc ggaagagatt
     1321 ttggattggt ttttgaagaa ttagttgctg gtcactggtt gctagttaac gagctccggt
     1381 ttggttcaca atttaaccag caacgagtaa cgagaaacgg agtttcatgg acatcgtaat
     1441 cgttggcagc gggcccgctg gcgttcatgc cgctcttcaa tgccggcaga gctggccccg
     1501 gaaatcggta actctgatcg agggggaaaa gagcatcgga acctgccgcc ccatgcttcc
     1561 tcagtttatg gcaggccagg taaaaggaga gaagctttat cttttcaggc ccgcggaaga
     1621 tcccctgctg gtgctgaaga ccgggaccaa ggttcgatcc ctggacaaag aaggaaagac
     1681 ggttcagctg gaaaacgggg agaccaccgc ttatgagcgg ctgatcctgg ctccgggagg
     1741 ccggcccatc atcccccgca tagagggaat cgatggactg gaggggctct tcccggtccg
     1801 caacctccct gaagccgaga aggtccacaa ctggattacc aaggatcaga aaatcgtggt
     1861 cctaggaggc ggactcgtag gagtcaaaac cgccgtttac ctccggaccg ccggattcca
     1921 aatcgcgatt gtggaaaagg aggatcatct ccttccccag gcgctaagag cagaaacagc
     1981 caaatatgta gaagagcact tccgccggat gggcatagac ctctacctgg gccagaccct
     2041 cgagtccgct gagggtgagt acggaaaaat caagagggca agagcaggag gacaatggat
     2101 cccctgccat gccctgcttc tggcgatcgg gtcagtcccc aatatttctt tcctcgaagg
     2161 gtccggtctt ctggaagagg gaaggttgcg ggtttcggcg gccctgcaaa cgaaggatcc
     2221 caggattctt gccgcaggcg atgcggtcac catctttacc ccggacggaa agagctatac
     2281 cccctggacg tggccccaag ccgtttccca ggggaaactg gccgccgcga acctataccg
     2341 gtcgaacccg cttcccctca aagccctgac ccgggtgaac tccatgaacc ttcagggtct
     2401 tgccctcaac atgctgggag ccttcgtgga ggggtctgag gagatctgtt atgcgccgcc
     2461 gttggagggc atcctacgcc aagtcttcct gcgggagggt cgaatcaccg gaggggcgct
     2521 ggtgggagac atcaccggtg cgggacctct gcatcatttg ataatccaaa gccaagattc
     2581 catttctgaa gtcaggagat tgatcaagcc ttccttccgc ctcatgtccc cgaacctgtc
     2641 ggagcggggg accggtcgca ggagggccag gcttattcct ggagaggaac gatagacatg
     2701 ctgattcgaa aagatctctg tgtgggttgc gggcggtgtc aaccctactg tccggtgggg
     2761 gccatccact tcgaagagct gaagagtatc gtggaccagg aggtctgcta cgaatgcggg
     2821 acctgcctgc ggtccgagat ctgtccggtg gacgccatcg aagaatctcc gtacgtgtat
     2881 gagtatccac gggcgttgcg gaaattcttc agcgacccca acgcgaccca cgccctcact
     2941 ggcatcgcgg gaagggggac ggaagaatca aagagcaatg acgtcatcaa ccgggtcgta
     3001 ccgggggaag tggggatggc cgtcgaattc ggaagaccca ccctgggaat ggacctgacg
     3061 aacatccaaa aggttacccg ggccatggcc aaagccggtt tcaccaagat cgagcccgaa
     3121 aaccccttca atgacatgat ccagaactat gaaagcggcg acctgaagcc ggaactcatg
     3181 ggcgagaagg ttctgagcgc gatcatggaa ttcaagatca cgccggatcg gctcggcctg
     3241 gctcttcgca ccatcaaaga agcggccaag aggtggacag cgtcttcgtg gtggatgtct
     3301 acacccggct ggagcccggg ctgaccgtcc ctaaggaaat cctcgagacg attgaagccg
     3361 aaggttactc gacgcgaacg aatgccaaaa tcaata
//
LOCUS       DASPRR010000396         5828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2343847, whole genome shotgun sequence.
ACCESSION   DASPRR010000396 DASPRR010000000
VERSION     DASPRR010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343847"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..986
                     /locus_tag="VEL68_14550"
     CDS             414..986
                     /locus_tag="VEL68_14550"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXZ37238.1"
                     /translation="MDKIKIDNNAFVYPMPMVLAGAMVGDKPNFMAVGWVARVNFQPP
                     MIGIALGKTHYTNEGIHKNQAFSVNIPGMDLIEKVDFCGLVTGKKTDKSRTFDVFYRE
                     LKNAPMIRECPLCMECRVVQGVDLPSNTLFIGQIMGAYAEERFLTDGQPDILKINPFT
                     LTMPDNQYWTVGKLAGKAWGIGKNWRGPKR"
     gene            1017..1814
                     /locus_tag="VEL68_14555"
     CDS             1017..1814
                     /locus_tag="VEL68_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ37239.1"
                     /translation="MEELVRVRREGEVALVLLNRPQKYNAFDSEMVEQFSTSLITLAV
                     EEGVKGLVVSGEGKAFCAGGDLQWASRFPAGSPSGFHQLAARFHQAILEIRRMGKPVI
                     AAIRGIAAGGGFSLALACDFRVMGRSAELRQAYTSNGLCIDGGGTFSLPRLVGLARAL
                     EIATFDQPISSGQALAWGLVTKVVEDERVLEEAFQMARQLAKGSLHSFAWSKRLLTDS
                     FQNSIEAQLENERHGLYACAAHEDGKEGLRAFGEKRKPVFGGRKPVT"
     gene            1868..2224
                     /locus_tag="VEL68_14560"
     CDS             1868..2224
                     /locus_tag="VEL68_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXZ37240.1"
                     /translation="MGFRTDSFQEKWVSSILDRIPMAVTIFDLRGIMLYYNEFAPSLI
                     NRKPELLGKDIRLCHQKPESNARIDWMIEEFRRGRRDPISYEAKPYGKTLLVTVSPLE
                     ADGRLLGCIHTAIPKP"
     gene            2480..4207
                     /locus_tag="VEL68_14565"
     CDS             2480..4207
                     /locus_tag="VEL68_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04190 family B12-binding domain/radical SAM
                     domain protein"
                     /protein_id="HXZ37241.1"
                     /translation="MLAKPDLVLLHAPSVYDFRKLPILFGPISDLVPSTPIFEMYPVG
                     FSSIAEHLERNGIRVRIINLAYRMLQEKEFDPERWIAKLRPQMFGIDLHWLPHAHGSL
                     EVAGLCKKRHPDIPVVFGGYSASYFHEELIRYPQVDFVIRGDSAEEPLRQLMVALREE
                     KPLESIPNLTWKDEKKEIHINPLNHVLSDLNEYSNNYPNLFRSVVRYLDLKSLIPIHD
                     WWEYPITAVMTVRGCTHNCVFCGGSHFGLSHFYRRDICAFRSPERVADDILAISRYTN
                     APIFVVGDLRQGGEEYAQRLLSRIKPVRPKNQVVLELFTPAPESYFQMVAEAFPNFNF
                     ETSPESHEEKVRKATGKYYSNEAMEKTIRYALDQGCSKFDVFFMIGLPEQTPASVLGT
                     LDYCEHLLKTFDRRLNPFISPLAPFLDPGCIAFEKADEVGYRVFCKTLEDYRKALLAP
                     SWKYTLSYETKWMTREDIVRSSYESGLRLNRLKEKYGLLDAATVDRTEKRILLAREMI
                     GKVDEIILLPEPERSRKLLALKETFDRASVSTVCDKDEIKWPVFRWRLNFPNILRAIF
                     QRPPRLRGA"
     gene            4296..4604
                     /locus_tag="VEL68_14570"
     CDS             4296..4604
                     /locus_tag="VEL68_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXZ37242.1"
                     /translation="MTQDKNDQSTEDPEPLELPIDGVLDLHTFQPREVEDLLQDYIVA
                     CREKGILEVRVIHGKGTGALRRTVHSILSRLPEVISFHLAGAEAGSWGATLVRLRPLE
                     "
     gene            5400..>5828
                     /locus_tag="VEL68_14575"
     CDS             5400..>5828
                     /locus_tag="VEL68_14575"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37243.1"
                     /translation="MGKRFFLLAVSGLLFLWALPQMAAAQDEVVIGIPLAITGAQAKF
                     GEQHKNGYTMALEEILAQGGIRTGALKGKKLTFHYEDDEGKPEKAKAVTEKLITRDKL
                     PIIMGGYASSEGYAIAGTTAQYGTPFLSPSGAADDITQKGW"
BASE COUNT         1365 a         1566 c         1594 g         1303 t
ORIGIN      
        1 aggaatttgg atcatcaggc tcggcgggcg cttatccttc catgaaactc ctccccatgt
       61 tccgcaggga gaggcctgag aggagattcc tccaagcaaa aaagagaacc aaagcatttt
      121 tccgtttcaa tccccccagg acaggcccga tcccatggaa gaaccaccgc ctcaggatga
      181 tgatccaaat ttctccaccc tgaaccccat gaggtccccc ctcctttcca gagaccttat
      241 tttcgaagga ctctttttta agaactgggg gtgggcaagg cgtcccagga gcttgacttg
      301 acctcttctt ttgattaaca tccttttaac caagtctcgc cggcgtggag aatggcttcc
      361 attttacgtg gggggctgca tccctccggg aacccaggat ttggaggtgg atcatggata
      421 aaataaagat cgacaacaac gcctttgtct atcccatgcc catggttctg gccggggcca
      481 tggtgggcga caaaccgaat ttcatggccg taggttgggt tgcccgggtc aattttcaac
      541 ctcccatgat cggaatcgct ctgggaaaga cgcactatac caatgaaggt atccataaga
      601 accaagcctt cagcgtaaac atccccggca tggacctgat cgaaaaggtg gatttctgcg
      661 gtttggtgac cggcaagaaa acggacaagt cccggacctt tgatgtgttt taccgcgagt
      721 tgaagaacgc ccctatgatc cgggaatgcc ctctctgcat ggaatgcagg gtggttcagg
      781 gggtggacct gccctccaac accctattta tcggtcagat catgggagcc tacgcggaag
      841 aaagatttct gactgacggg cagccggata tcctaaagat caatcccttt accctgacca
      901 tgccggacaa ccagtactgg acggtgggga agcttgccgg gaaagcctgg ggaattggga
      961 aaaattggag aggacccaaa cgctgaacaa gactcgggga ttcaaggagg ggcggcatgg
     1021 aggaactggt acgggtccgt cgcgaagggg aagtggccct ggtgctgctg aaccggcctc
     1081 agaagtacaa tgcctttgac tcggaaatgg tagagcaatt ctccacctcc ctgatcaccc
     1141 tggcggtgga ggagggagtg aaaggattgg tcgtttcggg ggaggggaaa gccttctgcg
     1201 ccgggggtga cctgcaatgg gcttcccggt tccctgccgg ctctccctcc ggttttcatc
     1261 aattggctgc ccgtttccat caggcgattc tggaaatccg ccggatgggg aagccggtga
     1321 tcgcggccat tcgcgggatc gccgccggcg gggggttctc cctggctctg gcctgtgact
     1381 tccgggtgat gggacggtcg gcggaattgc gtcaggcgta cacctcgaac ggcctctgca
     1441 tcgatggggg agggacgttc tcccttcccc ggctggtcgg cttggcccgg gcgttggaga
     1501 tcgcgacctt cgatcagccc atctcttcgg ggcaagccct ggcttggggc ttggtcacca
     1561 aagtggtgga agatgagcgg gttctggagg aagcgttcca gatggcccgc cagctggcca
     1621 aaggttccct gcattctttt gcctggtcca agcggcttct aaccgactcc ttccagaact
     1681 ccatcgaggc ccaactggaa aacgaacgcc atgggctgta cgcctgcgct gcccatgagg
     1741 acgggaagga gggactgcgg gctttcggag agaagcggaa gcctgttttt ggcggccgga
     1801 aaccggttac ctgagctaaa cttttgaccg gtggaatgaa ggtttaattc ggaagggaga
     1861 agggaacgtg ggttttagaa cagattcttt tcaagaaaaa tgggtctcct ccatcctgga
     1921 caggattccc atggccgtga ccatcttcga cttgcgggga attatgctct attacaacga
     1981 attcgccccc agcctcatca accgcaagcc cgagctcctc ggaaaggata tccgcctctg
     2041 ccatcagaaa cccgaaagca atgcccgcat cgattggatg atcgaggaat ttcggagggg
     2101 cagacgggac ccgatctctt acgaggccaa accttatggg aaaactctct tggttaccgt
     2161 atcccccttg gaagccgatg gacgcctcct cggctgcatt cacacggcga ttcccaagcc
     2221 ttgaaccgca accgacgatt tgagatttgt gcccgttccg cctctttctt tgccgttcga
     2281 tcccgtccca cgagacgata ccttcgcaat cactcttccc aaggatgagt ccgatccttg
     2341 cattctccaa attgatctgg tagcttcaaa aaacaaaaaa gcgatcggct ctcagcggtc
     2401 gactttcagc ccttagggcg atattcgctg atagctgatc gctaatcgct gaaggcggtt
     2461 attggaaacc ttttcaacca tgcttgctaa accagacctc gtcctccttc acgctccttc
     2521 ggtctacgac tttcggaagc tgccgattct cttcgggccc atcagcgacc tggttccttc
     2581 gacccccatt tttgaaatgt accccgtggg tttttcctcc atcgccgaac acctggagag
     2641 gaacgggatc cgggtgcgga tcataaacct ggcctaccgg atgctgcagg aaaaagaatt
     2701 tgatccggag agatggattg ccaaacttcg gccccaaatg tttggcatcg atctacactg
     2761 gcttccccat gcccacggtt cgctcgaagt ggcgggcctt tgcaagaaac gccatcctga
     2821 cattccagtc gtattcggtg gatattcggc ttcttatttt catgaagaac tgatccggta
     2881 tcctcaggtc gatttcgtga tccggggcga ttcagcggaa gaaccccttc gacagctgat
     2941 ggtcgcgcta agagaggaaa agcctctgga gagcatcccc aacctgacct ggaaagatga
     3001 aaaaaaggaa atccatatca atcctctcaa ccatgtcctt tccgatttga atgaatactc
     3061 caacaactat cctaatttat tccgctccgt ggtcaggtac ctggacctga aatccctgat
     3121 ccccatccat gactggtggg aatatcccat taccgccgtc atgaccgtcc gggggtgcac
     3181 ccacaactgc gttttctgcg gagggtctca cttcggattg agccattttt accgccggga
     3241 catctgcgcc tttcgcagcc cggaaagagt ggccgacgac atcctggcta tctccaggta
     3301 tacgaacgcc cccatatttg tggtcgggga cctgaggcag ggtggggagg aatatgccca
     3361 gcggcttctc tcccggatca agccggtgcg gccgaaaaat caagtcgtcc tggaattatt
     3421 cactcccgca ccggaatcct attttcagat ggttgcggag gcttttccca acttcaattt
     3481 tgaaacctcc cccgaatccc atgaggagaa ggttcggaaa gcaaccggaa aatattattc
     3541 caatgaggcc atggaaaaga cgattcgtta cgccctggat caaggctgtt cgaagttcga
     3601 tgtctttttc atgatcggcc ttcccgagca gactccggcg tcagtcctgg ggaccctaga
     3661 ctactgcgag cacctgctga aaaccttcga ccggcgcctt aaccctttca tctctccctt
     3721 ggccccattt ctcgatcccg gatgcatcgc ctttgaaaag gccgacgagg tgggatatcg
     3781 ggtcttctgc aaaaccctgg aggactaccg gaaggccctc ctggcaccca gttggaaata
     3841 taccctcagc tacgagacga aatggatgac ccgggaggac attgtccgga gttcttacga
     3901 gtccgggctc cgcctgaacc ggctgaagga gaaatacggc cttctggacg cggctacagt
     3961 cgaccggaca gagaagcgca tcctcctggc gagggagatg atcggcaagg tggacgagat
     4021 cattctactc ccggaacccg agcgaagccg gaagcttctg gccctgaagg agactttcga
     4081 tcgggcgagc gtgtccacgg tttgcgacaa ggatgagatc aaatggccgg ttttccggtg
     4141 gcggttgaat tttcctaaca tcctccgggc gatctttcaa cgacccccca ggctgagagg
     4201 agcgtagggg cgggttttaa acccgcccct acagccggtg gtaaggtaat ctcattcatg
     4261 ggggaaatcc atgaattgcc cctgccacgc caaaaatgac ccaagacaag aatgatcaat
     4321 ccacggaaga cccagaaccc ctggaactgc ccatcgatgg ggtcctggac ctgcatacct
     4381 ttcagcccag ggaggtcgag gacctcctgc aggattacat cgttgcctgc cgggaaaaag
     4441 ggatcctaga ggttcgggtg attcatggaa aagggacggg ggccttacgc cgaacggtgc
     4501 attccatcct gagccgcttg ccggaggtga tttccttcca tttggccgga gcagaggctg
     4561 ggagctgggg cgccaccctg gtaaggctca ggcctctgga ataaacttgg ttgcacccag
     4621 agaacgggcc tgtccaccgc ctcaactctt tccacccatt ctttccactc actatatctg
     4681 gggaaaaaat aggcgaacaa aacaagagaa agcaccatga ggggatagaa gtccagggga
     4741 ttgcgggtca ggaaaaagag gcttaacgcc gtgacggcca ccgtttcaca gagcagattc
     4801 gtcgccaggg aacagatcaa gagccgttta ggggagtttt tgaatgattt tcccgccccg
     4861 ccacagagga tttgactgcg cacaaaccag attagaaaaa gggtcatgac ggtaaaactc
     4921 agcagaaata ccttgatcgg aaaaatttct ggagaagagg aatccttttt caaaaaaggg
     4981 acgtcggatt tgatactttc aaccatcaac aggtatgcga aaatggtgaa tgccatggca
     5041 gtccacagaa gtacggcctg gcgataactt ttttttaaac ctcccacgcg attccctcct
     5101 ctctgtcttc aaagctgatc atcctttttt atcgaccgct cgaggaggta acttaaagaa
     5161 gccttggggg gttcagtccg ggatcttcga ggatgatttt tctccttgac tttccgcccc
     5221 aatttttgtt aaagatattc gggcctttcg tccccgcccc aaaatgtctg gccgggaacg
     5281 ggtttgaata gacggcatcc tttggttgaa tgaaacctga attgcaaatg acaggtcacc
     5341 ccagttcggc aagagctgtt gtggtgccgt ttcaaagacc aattgaaagg gaggaaaaga
     5401 tgggaaaacg gttttttctc ttggctgtgt caggattatt gttcctgtgg gcccttccgc
     5461 agatggccgc agcccaggac gaggtggtga tcggaattcc tctggccatt accggcgctc
     5521 aggcgaaatt tggtgaacag cataagaatg ggtatacgat ggccctcgag gaaattttgg
     5581 cccaaggtgg gatccgcacg ggtgcgctta aggggaaaaa attgactttc cattatgaag
     5641 atgatgaggg gaaaccggaa aaggccaaag ccgtgaccga gaagctgatc acccgtgata
     5701 agctaccaat aatcatgggc ggttatgcca gttcagaggg atacgccata gccggaacca
     5761 cggcgcaata cggtaccccc ttcctatctc ccagcggagc agcggatgat attacccaga
     5821 aggggtgg
//
LOCUS       DASPRR010000397         4605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_98082, whole genome shotgun sequence.
ACCESSION   DASPRR010000397 DASPRR010000000
VERSION     DASPRR010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98082"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEL68_14580"
     CDS             complement(<1..587)
                     /locus_tag="VEL68_14580"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ37244.1"
                     /translation="MIQILGIAGSPVREGNTEALLKEALGVTSSDPEIQTEIFHLSGL
                     KIEGCSHCNWCIKNQTPDKFCALPDGMDEIYPALLRADAVILASPVHIGRMSGLMANM
                     IDRMRVFVYGNAHRGKLTDKVGAALVVGFLRHGGLETTLNILNSTFAIFGMISVGRGG
                     MALTSLEGKGRVTKGVRHMVLEDGLGVLTAKGAVQR"
     gene            858..2021
                     /locus_tag="VEL68_14585"
     CDS             858..2021
                     /locus_tag="VEL68_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ37245.1"
                     /translation="MNFDLTEEQNLLRQAVRDFAEKEIAPLAEELDEKEEFSVELTLK
                     MGDLGLLGCFVPEKYGGSNMGYISYIIAVEELARVDGSQAATIAAGNSLGIGPIYYYG
                     SEEQKEEWLPRLCSGKMLAAFGLTEPAAGSDAGGSKTRAELRGDHWIINGSKIFITNS
                     ANSLTGVIVVQAITGQKEDGKRELSCLLVPKEAPGFIAREMKKKMMWRSSNTGELFFD
                     DCAIPRNDLLGKRGDGFHQMLAILDGGRLSIGAMGLGGAQGAFEMGLHYANTRVQFGR
                     PISSFQINAFKLADMAMEIELARNLLYKACWLRENTRPFGKEAAMAKLYCSEVMGRCV
                     NHAVQLHGGYGLMKEYKIERFYRDHKLLEIGEGTSEIQRIVIARNIGVAGRAI"
     gene            2102..2803
                     /locus_tag="VEL68_14590"
     CDS             2102..2803
                     /locus_tag="VEL68_14590"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXZ37246.1"
                     /translation="MKRIKGRKAASGEKIEKEFQRRQEDHPDLLTDPSHHRVPMPPPK
                     GPSRQTLGSRIHEARQLRGLTLQDLSSRTGISLETLEKVEANKMIPPLGELIKVGKAL
                     EMKMGYFISPGVEKPMTVVHSDKRPVVSRHGKKKSEQYGYFYESLAPEKADRFMEPFL
                     ITMIPSEVKELSSHDGQEFIFVLEGEMRAQVGDQVEILKAGDAVYYDSSHPHLVKCHG
                     DKPAKILAVLYTGSK"
     gene            2958..3794
                     /locus_tag="VEL68_14595"
     CDS             2958..3794
                     /locus_tag="VEL68_14595"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015179051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXZ37247.1"
                     /translation="MAKGIALNLGLNSVDPKHYGGWSGELNACEADAEDMASIAKAKK
                     FQVTKLLTKAATRARVLAQITKAALTLKSGDIFMLSYSGHGGQLPDINNDEPDAEDET
                     WCLYDGELVDDELYALWRKFAPGVRILVFSDSCHSGTVTKLAYYQGTVSLRTGAVGSK
                     EVHYRAMPPDVALRTYRQNKAFYDRILKAKVPEEKQPAVKATVLLISGCQDNQLSSDG
                     DFNGLFTAQLLKVWKNGAFKGDYKTFYKMIVKRMPPDQTPNYFKVGAANPKFESQKPF
                     TI"
     gene            4093..>4605
                     /locus_tag="VEL68_14600"
     CDS             4093..>4605
                     /locus_tag="VEL68_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37248.1"
                     /translation="MAGKHGHEDKVLNCIGSREKEKDWMFENARDAGLLDTRRKIPPE
                     KDLREKWWGVGDQKKTGSCVGWALADSVLRWHFVKADWLPRNEMLSPRFIWMASKETD
                     DFISQPTTFIEAEGTSLKAALDIARKFGVVGDSILPFSSEKLYPGEAKTFYAIASKLK
                     IASYFNLGLEL"
BASE COUNT         1113 a         1237 c         1279 g          976 t
ORIGIN      
        1 cgctggacag cgcccttggc ggttaaaacc ccaagcccgt cttccaggac catgtgccgg
       61 acgcccttgg tcactcttcc tttcccttcc aggctggtca gggccattcc gccgcggccc
      121 acggagatca ttccgaagat ggcgaaagta ctgttgagga tgttgagggt cgtttccaat
      181 cccccgtgac gcagaaagcc caccaccagg gcggctccca ctttatcggt cagttttcct
      241 cgatgggcgt ttccgtaaac gaaaacccgc atccgatcga tcatattggc catcaggcct
      301 gacatccgtc cgatgtgcac tgggctggcc aggatgaccg cgtccgctcg caggagggcc
      361 ggatagattt catccatccc atccgggagg gcgcagaatt tatcgggggt ctggttttta
      421 atgcaccagt tgcagtggct gcagccctcg atcttcagcc cggaaaggtg aaaaatttcg
      481 gtttgaatct ccggatcgga agaagtaaca cccagggctt ccttcaagag cgcctcggtg
      541 ttgccctccc tcaccggact tccggcaatc cctaaaattt ggatcataag tcagcctcca
      601 gcttattgcg atcggcggtc agcattcagc agtcagccgt ccgcgaatat tggtttttaa
      661 atctgtgctg aaagctgata gctgagtgct gatagctatt tttcctacca taaagcggcc
      721 ggagaatcaa gaatggtcgt tgtcttgagg cactttcttt atatgaaatt cttgacttca
      781 atgggagaaa aaaatatagt gaatccatct tcctgcgctg tctcttcaac gcccccgcga
      841 ggtgatgcac gatggccctg aactttgacc tcaccgaaga gcagaacctg ttgcgacagg
      901 ccgtccggga cttcgccgaa aaggaaatcg cccctctagc cgaggaactg gatgaaaaag
      961 aagaattttc ggttgagtta accctcaaga tgggagacct gggcctcctg ggttgttttg
     1021 taccggagaa atacggcggc tccaatatgg gctatatatc ctatatcatc gccgtagaag
     1081 agctggcgcg ggtcgacggg tcgcaggccg ccacaattgc agcggggaat tcgttgggga
     1141 tcggtcccat ctactattac ggcagcgaag aacagaagga ggagtggctt ccccggctct
     1201 gctccggaaa gatgctggcc gcctttgggc tcaccgaacc cgctgccggg tcagatgccg
     1261 ggggttcgaa gacccgggcc gaactgcggg gagatcactg gatcattaat gggtcaaaaa
     1321 tttttatcac caactcggct aattcgctaa ctggcgtaat cgtagtccag gccatcaccg
     1381 ggcaaaaaga ggatggaaaa agggagctga gctgtttgct ggtacccaag gaggctcctg
     1441 gtttcattgc gcgggagatg aagaagaaga tgatgtggcg ctcttccaac acgggagaac
     1501 tcttcttcga cgactgcgcc attcctcgga acgaccttct gggaaaaagg ggagacggat
     1561 ttcatcaaat gctggccatc ctagacgggg ggcggctttc catcggggcc atgggccttg
     1621 gcggggcgca gggcgccttt gagatgggcc tgcattacgc caacacgcgg gtccagttcg
     1681 ggcgtcccat ctcctccttc cagattaatg cctttaagct cgccgacatg gccatggaga
     1741 tcgagctggc ccgaaacctc ctctataaag cctgctggct gagggagaat acccgtccct
     1801 tcggcaaaga agcggccatg gccaaacttt actgctcgga agttatgggc cgctgcgtca
     1861 accatgccgt tcagctccat ggcgggtacg gtctgatgaa ggaatataag atcgagcgct
     1921 tttaccgcga tcacaagctt ttggaaatcg gcgaagggac ctccgaaatc cagcgcatcg
     1981 tcatcgcccg caacatcgga gtggccggaa gggcgattta agaagttaat cctggttatc
     2041 cgcagattac gcaaagatat ggaggtatct tgtccgcggt tgattctgga aggaggggga
     2101 aatgaagaga ataaaaggaa gaaaagcagc ctctggcgag aaaatagaaa aggaattcca
     2161 gcgccggcaa gaagatcatc cagacctgct cactgatcct tcccatcatc gggttccgat
     2221 gcccccgccc aagggtcctt ccaggcagac cctcgggtcg cggatccacg aggctcgcca
     2281 gctccgcggc ctcaccctcc aggacctgag cagccggacc ggaatcagtc tggaaaccct
     2341 tgaaaaggtc gaagccaaca aaatgattcc ccctttgggc gaactgatta aggtggggaa
     2401 agctttggag atgaagatgg gatatttcat ctccccgggt gtggaaaaac cgatgaccgt
     2461 tgtgcactcc gacaaacgcc cggtagtctc ccgtcatgga aaaaagaaga gcgagcaata
     2521 cgggtatttc tatgaatccc tggctccgga aaaggcggac cgcttcatgg agcccttcct
     2581 gatcaccatg atccccagcg aggtgaagga gctttccagc catgacggcc aggaatttat
     2641 cttcgtcctg gaaggggaga tgagagccca ggtgggggac caggtggaaa tcctaaaggc
     2701 gggcgatgcc gtctattacg actccagcca tccccacctg gtaaagtgcc atggggataa
     2761 accggcgaaa atccttgccg tcctctacac cgggtccaag taacgtcttt ctccctttta
     2821 atgggcactt agacaaactg aatcataact aaccacccag ccgattaact tggagaggga
     2881 ggtggattaa attgtccaag cctggtcttc ggaattttga aaccaggctt ttatttttca
     2941 aaaaaggagg gcttcccatg gctaaaggaa tcgcattgaa tctaggtttg aattcagtgg
     3001 acccgaaaca ttacggtgga tggtccggag aactgaatgc ctgcgaggcg gatgcggagg
     3061 acatggccag catcgccaaa gcgaagaagt ttcaggtaac gaaactattg accaaagcgg
     3121 ccacacgggc gcgggttttg gctcagatca ccaaagccgc tcttaccctg aaatccgggg
     3181 acatcttcat gctcagctat tccggacacg gcggccagct gccggacatc aacaacgacg
     3241 agcctgacgc ggaagacgaa acctggtgcc tctacgacgg tgaattggtg gacgacgaac
     3301 tgtatgcctt atggagaaaa tttgctcccg gagttcgcat cctggtcttc tccgacagct
     3361 gccacagcgg aacggtgacc aagctggcct actaccaggg gaccgtcagc ctgcggactg
     3421 gagcggtcgg ctcgaaagaa gtccactacc gggccatgcc ccccgatgta gccctccgca
     3481 cctaccggca gaacaaggcc ttctatgacc ggattttgaa ggccaaagtt cccgaagaaa
     3541 aacaaccggc ggtgaaggct acggttctcc tcatatccgg ctgccaggac aaccagcttt
     3601 cttcggatgg agacttcaac ggtctcttca cggctcagct cctgaaggta tggaaaaacg
     3661 gcgccttcaa gggagactac aagacgtttt ataagatgat cgtcaagcgg atgcctcctg
     3721 accagacccc caactatttc aaggtcggag ccgcgaatcc caaattcgag agtcagaagc
     3781 cgttcacaat ttagagcccg tagccctcct gtcaccgttc accctgagct tgtcgaaggg
     3841 tgcgtcgcta agccgttcat gcttcgacaa gcgttcgact gagctcacgc cgaaggctca
     3901 gcacgaacgg cttagccctc agcgtccata caattatgga ccgattagta aacctggacc
     3961 tcatttggcc tgaagggtac cggagcgatc cgggaaatca cttctcatct ctgcctcctt
     4021 tttcacaggt gtcacgactc tttttgagga catcaaaatg gcctttgaaa aaattcgaaa
     4081 aggaggagtc tcatggccgg aaaacatggg cacgaagaca aggttctgaa ctgcatcggc
     4141 tctcgggaga aggaaaagga ctggatgttc gaaaatgcca gggatgccgg cttattggat
     4201 acccggcgga aaattcctcc agagaaggac ctgcgggaaa aatggtgggg agtgggggac
     4261 cagaagaaga ccgggtcctg cgtgggctgg gctctggccg attcggtctt gcggtggcat
     4321 ttcgtcaaag ctgattggtt gcccaggaat gaaatgcttt ccccgaggtt catctggatg
     4381 gcctccaaag agaccgacga tttcatctcc cagcccacga ccttcatcga ggccgagggg
     4441 accagcctga aggcggccct ggatattgct cggaagtttg gggtcgtggg ggattctatt
     4501 cttcccttca gctcggaaaa gctatatccg ggggaggcca agaccttcta tgccatagcc
     4561 tccaagctca aaatcgccag ctacttcaac ctgggcctcg aactg
//
LOCUS       DASPRR010000398         4275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_902412, whole genome shotgun sequence.
ACCESSION   DASPRR010000398 DASPRR010000000
VERSION     DASPRR010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902412"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VEL68_14605"
     CDS             <1..326
                     /locus_tag="VEL68_14605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37249.1"
                     /translation="MGINVSLVGGITFGIGTALAAVAASLIAPILLIYPTMGGPVIGM
                     AFVIIILGGMGSFFGAVVGGYLIGLAETLFSSYVTTNYVEALIFGILVFVLAVKPTGL
                     FGKAH"
     gene            423..1388
                     /locus_tag="VEL68_14610"
     CDS             423..1388
                     /locus_tag="VEL68_14610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37250.1"
                     /translation="MGKKSYCFLLTVLLMLAPLVITGQYPQHIMVMCGIWVIASLALN
                     LLLGYTGQLNLAHGTFFGIGAYATALLVQKLGFSFWLALPLGCAITAFMGFLTGLPTL
                     RTKGHYFPIATMCLGIAIYYVIARWDDFTGGARGLFGLPRPNPIPLPFGGNISFETTI
                     ACYYLVLFFVFLTYWVISRLVKSLAGRRLVAVRGNEDLAQSVGINTMQSKILSFTIST
                     FFAGLAGGLFSAYMGTLMAEQAHYLLSFEMLIYVMIGGIGTMAGPVIGGILLPILGEI
                     LQPLAGFRLILFGLLLILIIRFLPFGIVGGLRMARIRQARKKGAS"
     gene            1385..2173
                     /locus_tag="VEL68_14615"
     CDS             1385..2173
                     /locus_tag="VEL68_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37251.1"
                     /translation="MSEVLLRAQGVSKDFGGLRAVQDLDFQIYRGEVFSIIGPNGSGK
                     TTTFNLVTGFLPVTGGEISFKEDRITGLKPFQVCRKGIARTFQLTTLFDRNTVLENLI
                     IGQRATRESGILSCIFGSPGARQREGEALEKAHELAEFIGLARQKMIPAGALPQGSQK
                     QLSIGLALASAPELLLLDEPVGGVNMEEIGLLIDLIIRIRKSGITICLIEHKMSVVMN
                     ISDRVMVLSYGEKIAEGSPAEVSQNEKVIKSYLGERYAASRRQG"
     gene            2148..2858
                     /locus_tag="VEL68_14620"
     CDS             2148..2858
                     /locus_tag="VEL68_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37252.1"
                     /translation="MLLRVDKVESFYGRSQILHGVSLEIKEREFVCVLGANGAGKTTL
                     LKTIAGLAKPRKGSVEFAGRRIDALPAHQIVQEGITLTPEDRKIFPQMSVYKNLILGA
                     WVHGRNGEKIQKNISQAYDLFPILKDRRDQMAGTLSGGEQQMLVIGRSLMSNPKLLML
                     DEPSLGLAPLVVERIFEIIQEIHRRSSTILLVEQNASMSLATAGRGYILENGRIILEG
                     PAKNLLEDEKVKKAYLGL"
     gene            complement(2877..3470)
                     /locus_tag="VEL68_14625"
     CDS             complement(2877..3470)
                     /locus_tag="VEL68_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ37253.1"
                     /translation="MEKRGAVMKITCVLGSPRRNGNSEVLAKRFGDTAAKLGAQVQTF
                     VLNELHYRGCQACMACKTKSDKCVLQDDLTPVLESVREGDVLVMATPVYLWDVSSLLR
                     GFIERTYSYFVPDFATNPNPSRLKPGKKLVFIQTQGQPGENMFADLFSRIEPIFKRYG
                     FTDNHLIRGLGVRKLGEVGSREEVMKRVEETARKIVG"
     gene            4042..>4275
                     /locus_tag="VEL68_14630"
     CDS             4042..>4275
                     /locus_tag="VEL68_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37254.1"
                     /translation="MKRIGVVVLAVGLLAFLTSCGQKPTKEIDETTAAMNTVISEGLG
                     KYAPEDEKKLKDSMAAAMEEIKVQEGKTFKNFDK"
BASE COUNT          949 a         1139 c         1177 g         1010 t
ORIGIN      
        1 tgatgggcat caacgtcagc ctggtgggag ggattacctt cgggatcggg acggcgctag
       61 cggccgtggc cgcgagcctg atcgctccga tcctcttgat ctatcccacc atgggagggc
      121 cggttatcgg catggctttc gtaatcatca tcctcggcgg aatgggaagc ttctttggag
      181 ccgtggtggg cggatacctc atcggactgg cggagaccct tttctcttcc tatgtcacga
      241 cgaattacgt ggaggcgctc atcttcggga ttctggtctt cgtcctggcg gtcaaaccga
      301 cgggattgtt cggaaaggcg cattagagaa agcaggcttc aagatacagg tatcaggatt
      361 caggattatg cattctgacc ggcaacaggc aaccgggaac ggcgaaggca ttttggttat
      421 tcatgggtaa gaaatcttat tgtttcctcc tgaccgtact tttaatgctc gccccgctgg
      481 tcattaccgg gcagtacccc cagcacatca tggtcatgtg cgggatctgg gtcatcgctt
      541 ccctggctct caacctcctc ttgggataca cagggcaact caacctggcc cacggaacct
      601 tcttcgggat cggcgcctat gccacggcgc tcttagtcca aaagttgggt ttttccttct
      661 ggctggccct ccccctcggc tgcgcgatta ccgccttcat gggattcctg accgggctgc
      721 ccaccctccg gacaaagggg cactatttcc ccatcgccac catgtgcctg gggatcgcga
      781 tttactatgt catcgcccgt tgggatgact ttaccggcgg ggcccgggga cttttcggcc
      841 tgccccggcc gaatccgatc cccctgccat ttgggggaaa tatctccttc gagactacga
      901 tcgcctgcta ctacctggtg ctctttttcg tcttcctcac ttattgggtt atctcccgtc
      961 tggtgaaatc cctggccggg cggaggctgg tggcggtgcg agggaatgaa gacctggccc
     1021 aatctgtggg catcaacacc atgcagagca agattctcag cttcaccatc agcactttct
     1081 tcgctggcct ggcagggggc ctcttttccg cctacatggg gacactgatg gccgaacaag
     1141 cccactacct tctcagcttc gagatgctca tctacgtcat gatcgggggg atcggcacca
     1201 tggccggccc ggtcatcggg ggaatccttc ttcccatcct gggggagatt cttcagccat
     1261 tggccggttt tcggctgatt cttttcggtc tgctgctgat cttgatcatc cggttcctac
     1321 ccttcggaat cgtggggggc ctgcggatgg cacggattcg ccaagctcgg aaaaaggggg
     1381 cttcatgagc gaagtcctgc ttcgggcaca aggtgtgtca aaggatttcg gaggccttcg
     1441 ggcggtccag gacctggatt ttcagattta ccggggggag gtcttcagca tcatcggccc
     1501 caacggttcc gggaaaacga cgaccttcaa tctggttaca ggttttctcc cggtcaccgg
     1561 cggagagata tccttcaaag aggatcgaat caccgggctg aagcccttcc aggtttgccg
     1621 caaagggatc gctcggacct ttcagctgac cactctcttc gacaggaaca cggtcctgga
     1681 aaatctaatc atcgggcaga gggccactcg ggaatcagga atcttgagct gcatcttcgg
     1741 cagccccggc gccaggcaga gggaagggga agccttggaa aaggcccatg agctggctga
     1801 gttcataggc ctcgcccggc agaaaatgat ccccgcaggg gcccttcccc agggaagtca
     1861 gaaacagttg tccatcggcc tggcactggc atccgctcca gagctgctcc ttctggatga
     1921 gcccgtggga ggggttaaca tggaggagat cggcttgctc atcgacttga tcatccggat
     1981 ccggaaatcc ggtatcacca tctgtctgat cgaacacaag atgagcgtgg tcatgaacat
     2041 ctccgaccgg gtcatggtcc tcagctacgg ggaaaaaatc gccgaggggt cgccggccga
     2101 agtgtcccag aacgaaaagg tcatcaagtc ctacctgggc gaaagatatg ctgcttcgcg
     2161 tagacaaggt tgagagcttt tacggtcggt cccagattct gcacggggtt tccctggaga
     2221 tcaaggagag ggaatttgtc tgtgtcctgg gagccaacgg ggccgggaag accacccttc
     2281 taaagaccat cgccggactg gcaaaaccgc gcaaaggctc ggttgaattt gcgggacgga
     2341 gaatcgatgc gctgcccgcc catcagatcg tgcaggaagg gattaccctg actcccgaag
     2401 accggaagat cttcccccag atgagcgttt acaagaatct tatcctggga gcctgggtcc
     2461 acggccggaa cggggaaaaa attcagaaga atatatccca ggcctatgac ctctttccca
     2521 tcctgaagga tcgccgtgac caaatggccg gcaccctgag tgggggtgag cagcagatgt
     2581 tggtcatcgg ccgcagcctg atgtctaatc ccaagctcct catgctggac gaaccctccc
     2641 tgggactcgc ccccctggtg gtggaacgaa tttttgaaat catccaggag atccaccgcc
     2701 gctccagcac gattttgctg gtggaacaga acgcctccat gagtcttgct acggcgggcc
     2761 gcggctacat cctggaaaac gggcggatca tcctggaggg accggccaag aacctgcttg
     2821 aggatgaaaa agtcaagaaa gcctatttgg gattatagat tttgccctgt tctgcttcag
     2881 cccactattt tccttgcggt ttcctccacc cttttcatga cttcttcccg gctccccacc
     2941 tcaccgagtt tccgcacccc caatccccgg atgaggtggt tgtcggtaaa gccgtatcgc
     3001 ttgaaaatag gctcaatcct cgaaaaaaga tcggcgaaca tgttttcgcc gggctgcccc
     3061 tgggtctgga tgaatacgag ctttttgccg ggtttcaaac ggctgggatt cgggttggtt
     3121 gcgaaatcgg ggacgaagta ggagtaggtc cgttcaatga aacccctgag aaggctgctg
     3181 acatcccaca ggtaaacagg ggtagccatg accagcacat cgccttcccg gacgctttcc
     3241 aggacaggag tcaagtcatc ctggagaaca cacttatccg atttggtctt gcaggccatg
     3301 cacgcctggc agccccggta atggagttca tttaagacaa aggtctgaac ttgggcgccc
     3361 agctttgcgg cggtatcccc gaaccgtttc gccaggacct cactgttgcc gtttctccgg
     3421 ggacttccaa gcacacaggt aatcttcata actgctcctc tcttttccat ggaaatttcc
     3481 agcataagga ttgtaatcca acgtaacacg gcaggtgaag agggtcaagc tgcgaaatca
     3541 gggggatagg ggttagttcc cgtcagggta ggtaggtgat gagaagagaa atcccccggc
     3601 tgattttttc tgcacaccta cccttgcgcc gggggcacac cttaggcagg gaaataacct
     3661 agaattttgg aaggggagag taaagccgca aaagaacagt aagtcagagc tctcctatct
     3721 ctatcgcttt ctcctaagta aattgtggat ttctctttcg gaaatggatc tcaattattt
     3781 ctcgtatttc ctcatccatc cattggcacg gaattccttt ggaaactcaa tagccctcct
     3841 cagattttcc tgagctttga atcgtctttt tttctttcga acagtttacc atagagggaa
     3901 ggatcaaatt ccatatggaa tctcatccca gtgctttggc ttgaaacacg ggctgaaaaa
     3961 ttacttgaca tgcgccttcg atttagatag atattgtttt atcaggtatt tatcaaaccc
     4021 ttttgggaag aggagaagaa aatgaaaaga attggagtgg ttgtcttggc tgttggttta
     4081 cttgcattct taacgagctg tggccaaaaa cctacaaaag agatcgatga aacgacggcc
     4141 gccatgaaca cggtcatttc ggaggggctt gggaaatatg ctcccgagga tgaaaagaaa
     4201 ttaaaggatt caatggcggc ggcaatggag gagattaagg ttcaggaagg taagactttt
     4261 aaaaattttg acaag
//
LOCUS       DASPRR010000399         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_903230, whole genome shotgun sequence.
ACCESSION   DASPRR010000399 DASPRR010000000
VERSION     DASPRR010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903230"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..2260
                     /gene="lptD"
                     /locus_tag="VEL68_14635"
     CDS             233..2260
                     /gene="lptD"
                     /locus_tag="VEL68_14635"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HXZ37255.1"
                     /translation="MIPLPKYASPQEPWRLRADSVSFDQATNTYVAEGRVEIWQEKQK
                     LTANRVVVNATTKEAEATGNVTLVQGVDTLKSERMAVDLNTGLGIVVEGTLFLKNQNY
                     YLRGEEIERVGENTYRVKRGSFTTCNGDWPAWRFTGNEALITLEEYADLWGATFQVKN
                     LPILYSPYLFFPVKAERQSGFLFPLIGFSNVAGAKLQLSYFWAIARNMDATLYLDLAT
                     QKGIGEGLEYRYIRKEDSSGSLYAYHILESEEYRKKYTDPLDRKPDRWQVDFRHEEYI
                     DPTFFAKTRLRAFSDREYFVDYGTTYGTQSAEQAYSFLSLTKNWERYSFYGEARRTVD
                     LRQEDKTTLQYYPVTNFVGIRRPVLSSPLFFNFDSSYGYFWREQGTTGHRVDFFPRIS
                     LPLKWGGVEFNSELGGRETWYAGVSEGDESRSRTLWDFQTGLAADMYRVFDTGIEGVP
                     KIKHVLRPEITYTYIPDVDQTNIPYYDQPVPKTNAIFYGFTNRIIGKIVQGSTSRYHE
                     YVYFKIGETYDINEAIRTAGLSTQPRRPFGPISAELRIRDPKYITLENITNYDPNTNQ
                     LQTSYTSLGLNDWRGDGLTLQHTWGKGISDQVYTSLRIRVFSFLDLIYGKQYDFFAHQ
                     NLQTTYTVLYRHQCWSLELDYSERPGVAGGPAERKFWFMFNLQGLASFGQK"
     gene            complement(2467..>3037)
                     /locus_tag="VEL68_14640"
     CDS             complement(2467..>3037)
                     /locus_tag="VEL68_14640"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXZ37256.1"
                     /translation="KEGFLSITFEPYAREGGVLNLPDIAAVRKVVDPVPDDRVMGDLD
                     ALVAYVKKHPAARADRVGVTGFCRGGMYTLLFAAHSREVKAAVPWYGPIKPAKAPGIR
                     TAGPLDLAARISAPVLGLYGGEDLGIPVADVKEMETALKAAGKTAEFVIYPGAPHAFY
                     ADYRPSYREEAAKDAWGRCVAWFNKYLKI"
BASE COUNT          664 a          869 c          835 g          669 t
ORIGIN      
        1 cgctatgctt tttgcaaaat gcggtttctg atgattcttt gctttgccct gtgcgcccct
       61 cttatttttc tttatgccct atgttctatg tgctatgcgc tttttctatt cgttggtagg
      121 aggctcggat ttcaaaccgg gtaagagtag gggtctcggc caacggtggg tcctgacctc
      181 ctctttgctg gtcttccttc ttttggccct tctcccgagg gcccactccc agatgatccc
      241 cctccccaaa tatgcaagtc cccaagagcc ctggaggctg cgggcggatt cggtttcctt
      301 cgaccaggcc acgaatacct atgtggccga gggacgggtg gaaatctggc aggagaagca
      361 aaagctgact gcgaatcgag tggtggtgaa cgcgacgacg aaagaggccg aggccacggg
      421 gaatgtgact ttggtccagg gggtagacac cctcaaaagc gagaggatgg cggttgacct
      481 aaacaccggc ctggggatcg tcgtcgaggg gacccttttc ctgaagaatc agaactacta
      541 tctcagggga gaagagatcg aacgggtggg agagaatacg taccgcgtga aaaggggaag
      601 tttcaccacc tgtaacggtg attggccggc ctggcgcttc accggaaacg aggctctaat
      661 caccctcgaa gagtatgcag atctttgggg ggccaccttc caggtcaaaa accttcccat
      721 cctctattcc ccctacctct tctttccggt gaaggccgag cgccagtccg ggtttctgtt
      781 ccccttgatc gggttttcca acgttgccgg cgccaagctg caattgtctt atttctgggc
      841 catcgctcgg aacatggatg ccaccctgta cctggatctg gccacccaga aaggaatcgg
      901 ggaaggcctt gaataccggt atatccgcaa ggaagacagc agcggctccc tttacgctta
      961 ccacatcctg gagagcgagg agtaccggaa aaaatacacc gaccccctgg accgcaagcc
     1021 ggaccgctgg caggtggatt tccgccatga ggaatatata gaccctacct tcttcgccaa
     1081 aacccgcctg agggccttca gcgaccgcga atatttcgtg gattacggaa cgacctatgg
     1141 aactcaatcc gccgagcagg cctattcctt cctctcgctg accaagaact gggaacggta
     1201 cagtttttat ggggaagccc gccgtaccgt agacctccga caggaggata aaaccaccct
     1261 ccagtattat cccgtgacga atttcgtcgg aatccggcga cccgtgcttt cctctcccct
     1321 gtttttcaac tttgattcga gctacggata cttttggagg gagcagggaa cgacgggcca
     1381 tcgggtggac ttttttcccc gcatttccct tccgctcaaa tggggggggg tggagttcaa
     1441 cagcgagctg ggaggaaggg agacgtggta cgcgggggtc agtgagggcg atgaatctcg
     1501 cagccgtacg ctgtgggatt tccagaccgg gttggccgca gacatgtatc gcgtctttga
     1561 tacgggaatt gagggggttc cgaagatcaa gcatgtcctt cggcctgaaa tcacctatac
     1621 ttacattccc gatgtggatc agaccaatat cccctactat gaccagccgg tgcccaagac
     1681 caacgccatt ttctacgggt tcaccaaccg gatcattgga aagattgtgc agggatcgac
     1741 cagccggtat cacgaatacg tatacttcaa aatcggggag acatacgata tcaatgaagc
     1801 cattcgcacc gcggggttgt cgacccagcc ccgaagaccc ttcggaccta tttccgcgga
     1861 actgcggatt cgggacccaa aatacataac cctggaaaat attaccaact acgaccccaa
     1921 caccaatcaa ctccagacgt cttatacttc cttgggcctg aacgactggc ggggggacgg
     1981 gttaaccctg cagcatacct gggggaaggg gatctcagat caggtgtata cgtctctccg
     2041 gatcagagtc ttttctttcc tggaccttat ctatggaaag caatacgact tttttgctca
     2101 tcaaaacctg cagaccacct acaccgtttt ataccgccac caatgctgga gcttggaact
     2161 cgactattcc gaaaggcccg gagttgccgg aggcccggct gagaggaagt tctggttcat
     2221 gttcaacctt cagggcttgg cttcgtttgg gcagaagtaa aaaacctccg tccaaaaaat
     2281 ccccggctat gccgggggac tcgcagagcc tgacatttcc tggattcctt ggccgtggcc
     2341 cgggggcaag gggctggggt tcaaaaagca tcggccgggc gatccctatt cctggaggcc
     2401 ggcactggct tccgctcgag ccgaaggccc gatcgggtga ctagtaaaaa attcaaaacc
     2461 ttatatctaa atttttagat atttgttgaa ccaggccacg caacgacccc aggcatcctt
     2521 ggccgcctct tcccggtagc tgggccggta atccgcgtag aaggcatggg gcgccccggg
     2581 ataaatcacg aactcggccg ttttgccggc tgccttgagg gcagtctcca tctccttaac
     2641 gtcggccacg gggattccca ggtcctcccc gccgtaaagc cccaggacgg gggcgctgat
     2701 ccgggcagcc aaatccaggg gccctgcggt tcggattccg ggtgctttgg ccggcttgat
     2761 cgggccatac caggggaccg cagctttcac ttcgcggcta tgggcagcaa agagaagagt
     2821 gtacatccca ccccggcaaa atccggttac cccgactcgg tcggcccgcg ccgccggatg
     2881 cttcttgacg taggccacca gggcgtccaa gtctcccatc acccggtcgt caggaaccgg
     2941 gtcaaccact ttgcgcaccg cggcgatgtc gggcaggttg agaacccccc cttccctggc
     3001 gtaaggttca aaggtaatac tcaggaaccc ttccttt
//
LOCUS       DASPRR010000400         4220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2345191, whole genome shotgun sequence.
ACCESSION   DASPRR010000400 DASPRR010000000
VERSION     DASPRR010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345191"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..2208
                     /gene="rpoD"
                     /locus_tag="VEL68_14645"
     CDS             478..2208
                     /gene="rpoD"
                     /locus_tag="VEL68_14645"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXZ37257.1"
                     /translation="MEKFSQLIKMGKEKGFLTYAELNEFLPPGVVSADQIDEIITKIE
                     KMNIELVDVPPKTEKHVAADEETSLSLEEEEPDVDMEDRKPQDPVSLYLREIGAVPLL
                     TREKEVEIAKKIEEGERELIDAVSSIPLYLEDMDHLQEKLKSLEKSSEKSSPGTEEES
                     LFEEELDVKAILAKVSDIKKLGKQARRTRERLGRKRTPEEDRRNLEFELREMQDKIIL
                     SLKEVNRKTQFLDAIIGKLKGLGERAGRVEDEILDIQDQARLPIDHLRRLFRLKKKNR
                     REFRKRLAELKLKGEALEQYDQALKKAQAKLAKIEKEAHLPIGELKRLLQTIYRAEIK
                     AEMGRNEMIKANLRLVVSIAKKYINRGLHLLDLIQEGNIGLIKAVEKFDYRRGHKFST
                     YATWWVRQAITRSIADQSRTIRIPVHMNETINKLLKVSHNLLQKIGREPTAEEIAKRM
                     DLPVDKVRNILRIVREPISLDTPIGEEQEDFLGDFIEDQKTVSAMEAMTNMDLSKRAR
                     AVLSTLTPREEKVLRMRLGIDEEGEHTLEEVGETFSVTRERIRQIETKALRKLRHPSR
                     SRTLRAFVEG"
     gene            complement(2258..3412)
                     /locus_tag="VEL68_14650"
     CDS             complement(2258..3412)
                     /locus_tag="VEL68_14650"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ37258.1"
                     /translation="MAGRYEFQITTQIIYGRDSAVRIGEVANRLGLKKVQLITDAGVV
                     KSGVAGRVQQLLTGSGIQTVLFDRVEYNPTVQTADAAKKLFTDQKCDGVVAVGGGSPM
                     DVGKSVGILATNPGSAAEYLGIGKVKNRGASVICLPTTSGTSAEITDVAVLSEPEKKT
                     KMGLRSPQVAPSVAILDPMLTLTLPPAPTRESGLDALAHAIESYFCLNAWTPTEALTL
                     KGIELIGRHLRRATHDGSNYEARDGMMMGSLLAGMGLHTTWVNLVHAITGPLGGFYNL
                     PHGAANAIVLPHAMRFLLPGAVPKFAEISRALGEKTEHLSERQAAEKAIEAVEQLSRD
                     TGMPPGLSVYGVKEADLPKLSETIGASVLLPLCPRVPTAKDILEICRAAL"
     gene            3771..3971
                     /locus_tag="VEL68_14655"
     CDS             3771..3971
                     /locus_tag="VEL68_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXZ37259.1"
                     /translation="MPRGRVKWFNEQKGFGFISQDGGDDLFVHFSSIKQEGFKVLHEG
                     DEVEFDIAQGKKGLQAVDVVKR"
BASE COUNT         1085 a         1071 c         1127 g          937 t
ORIGIN      
        1 cctgcggaac atggggagga gtttcatgga aggataagca cccgccgagc ctgatgatcc
       61 aaatttctcc accctgaacc ccgacaagca cgaaaaactg ggggtgagaa aggatttcat
      121 gtggtttttt caggggatat gagtcaattt cgaaccacct ggtgattcag gaggcgtgaa
      181 atccagtttc atccacccgg ccttgattag gccgtacctg ctaagttcat tttagtaaaa
      241 gaccccactg gctggaaagc gcatctaatt attaaaaagg cctttcatcg cctggcaaat
      301 ttaataaaag agcttatatt ttctctatag gcaaatgctc cggtgatgaa agaggaaaat
      361 cgagggtatg cgagggcatt ttttaaagcc aaacgaaaaa tcgccgatat gtctttgttt
      421 gggccctctt aaaatttaac tctttaattc cttttccttt tttggaaggt cagctaaatg
      481 gaaaagttta gccaattgat aaaaatgggt aaagaaaagg gttttttgac ctacgcagag
      541 ctgaatgaat tccttccccc tggggtcgtc tctgcggacc agattgacga aatcattacg
      601 aaaattgaga agatgaacat tgaattggtg gatgtgcctc caaagacgga aaagcacgtt
      661 gctgccgacg aggaaacgtc actttccctt gaggaggagg aacccgatgt tgacatggag
      721 gatagaaaac ctcaggaccc ggtatccttg tatcttcgag aaatcggcgc cgtgcctctt
      781 ttaacccggg aaaaagaagt cgaaattgct aaaaaaatag aggaagggga aagggaattg
      841 atcgacgccg tatcgtccat tcccctttac ttggaagata tggaccattt acaggagaaa
      901 ttgaaatcct tggaaaagtc ttcggaaaag tcgagcccgg gaacggagga ggaatcccta
      961 ttcgaagaag aattggacgt gaaagccatt ctggcaaaag tttctgacat taagaagttg
     1021 ggaaagcaag cccggaggac ccgagaacgc ctggggagga agaggacccc cgaagaagat
     1081 cgtcggaacc tggaattcga gctccgggaa atgcaggata agattatcct gtcgttaaaa
     1141 gaagtcaacc ggaagactca atttttggac gcgatcatcg gaaaattaaa aggtctggga
     1201 gagagggcgg gaagggtgga ggatgaaatc ctggatattc aggaccaggc ccggcttccc
     1261 atcgaccacc tccgcaggct ttttcggttg aagaagaaaa atcggcggga atttaggaag
     1321 aggcttgccg aacttaagct caagggagag gcgcttgagc aatatgatca agctctcaag
     1381 aaagctcagg ccaagttagc caagatcgaa aaggaagccc atctgccgat tggggagctc
     1441 aaacgcctgc tccagacgat ctaccgggct gagatcaagg ccgaaatggg acgcaacgag
     1501 atgatcaagg ccaatttgcg tttggtggtc tccatcgcca aaaaatacat caatcgtggg
     1561 cttcaccttc tggacctgat ccaggaggga aatatcgggc tgatcaaagc ggtcgaaaaa
     1621 ttcgactacc ggcgggggca taagttcagc acctacgcta cctggtgggt ccgccaggcc
     1681 atcacccgat ccattgccga tcagtcccga accatccgaa ttccggtaca tatgaacgaa
     1741 actatcaata agcttctcaa agtctctcac aacctgcttc agaagatcgg gcgagaaccc
     1801 actgcagagg aaatcgccaa gcgaatggac ctgcccgtgg acaaggtccg caacatcctg
     1861 aggattgtcc gcgagcccat ctctctggat actccgatcg gggaagagca ggaggatttt
     1921 ctcggggatt tcatcgaaga tcagaagacc gtttcggcca tggaagccat gaccaacatg
     1981 gacctcagca aacgggcccg ggcggttttg tctacgctga cccctcgaga agaaaaagtg
     2041 ctgaggatgc gtttgggcat tgacgaagaa ggagaacata ccctggaaga ggtgggagaa
     2101 accttctccg taacccggga gcgaatccgc cagattgaga ccaaagccct ccgcaagctg
     2161 cgtcatcctt cccggtcaag aaccctgcgg gcctttgtcg aagggtgaag gtccaatcat
     2221 cgtccccctc gatccccgat tctcttccca tggggattca gagagccgct ctacagattt
     2281 caaggatatc ctttgcggtt gggacgcgcg ggcaaagggg gaggaggacg ctggcgccga
     2341 tcgtctcaga tagcttcggc aaatcggcct ccttgactcc gtaaaccgac aagcctgggg
     2401 gcatccccgt atcccgggac aattgttcca ccgcctcgat ggccttttct gctgcctggc
     2461 gttcggagag atgttccgtc ttttccccca gcgcccttga gatttccgcg aatttgggga
     2521 cggctcccgg cagcaaaaac ctcatggcat ggggcagaac aatggcattg gccgccccgt
     2581 ggggcaggtt gtaaaaccca cccaggggtc cggtgatggc gtgcaccaga ttgacccaag
     2641 tcgtatgaag acccattccg gccagcaagc tgcccatcat cattccatcc cgtgcttcat
     2701 aattgcttcc atcatgggtc gctctccgaa gatggcgccc gatcaactcg atgcccttga
     2761 gggtgagcgc ctcggtgggg gtccaggcgt tgaggcaaaa ataggattcg atggcgtggg
     2821 ccagggcgtc aagacccgat tcccgggttg gggccggggg cagagtcagg gtaagcatcg
     2881 ggtcgagaat cgctacggag ggagcgacct gggggctccg gagtcccatt ttggtcttct
     2941 tctccggttc actcaaaacc gctacatccg taatctccgc cgaggttcct gaggtagtag
     3001 gcaggcagat caccgaagca ccccggttct tcactttgcc gattcctaaa tactcagcgg
     3061 cagaaccggg attggtggcc agaatgccca cactctttcc cacatccatg gggctgcccc
     3121 cgcccaccgc aacgactccg tcacattttt ggtccgtgaa cagcttcttg gccgcatccg
     3181 ccgtctgcac cgttgggtta tattccaccc gatcgaacaa aaccgtttga attccgctgc
     3241 cggtcagcag ctgctgaacc cgacctgcaa ctcctgattt gaccactccg gcatcggtaa
     3301 tcaattgaac ttttttcagt cctagccgat tcgcgacttc tccgatccgg actgcgctgt
     3361 cccttccata gataatctga gtggtgatct ggaattcata cctgccggcc attgttaact
     3421 cctttccacg cggaaatttt ttgatccttt cctgggagga ttcctctggt tgaggatcaa
     3481 aacggcgacc atcagaatgg ctctggctgc cgggccaccc aggattcatt ctcgattttt
     3541 ttcttctact tatatgagag agggaaagct gtcaagctaa aataatgtcc ccgccaataa
     3601 atttgccttg acagggcggg ggcgaaaata tagtataaaa acataagttg cacagagttt
     3661 aggtttggaa ccaaaacagg ccgcagggct ctcgaagctt atgcggcttt ttttatcctg
     3721 ccccggctcc gctgacctat gaatgcccaa agaaaaggag gtgaaaagca atgcccagag
     3781 gccgagtgaa atggttcaat gagcagaagg gatttgggtt tatctcccaa gatggtggcg
     3841 acgatttatt cgtgcacttc tcatccatta aacaagaagg tttcaaggtt cttcatgaag
     3901 gggacgaggt cgaattcgac atcgcccagg gaaagaaggg ccttcaggcc gtggatgtgg
     3961 taaagcgcta agatttcgac gggccgaact tcagcccccg gggtttattc ccccggggga
     4021 tcctccatcc caggattttt gttgcccggc ccattccagc ccatgggggg aaacatgaac
     4081 actaagatct atgtgggaaa catcgccttt cagaccaccg aagccgatct gaaaacccat
     4141 ttctcccaag ccggagatgt ggtctccgtc aagatcccga aagaccatca gagcggccat
     4201 tcccgggggt tcgcctttgt
//
LOCUS       DASPRR010000401         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_99684, whole genome shotgun sequence.
ACCESSION   DASPRR010000401 DASPRR010000000
VERSION     DASPRR010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99684"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /gene="porA"
                     /locus_tag="VEL68_14660"
     CDS             complement(<1..357)
                     /gene="porA"
                     /locus_tag="VEL68_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HXZ37260.1"
                     /translation="MTDKRVGMEVSIAAAEAVKLARAEAISAYPITPQTHIVEHLSEL
                     VANGELDAEFVPVESEHTALSVCAGTSAVGARTYTSTSAQGLELMHEILFVTAGMRLP
                     IVMSVANRALSAPLSIW"
     gene            complement(354..647)
                     /locus_tag="VEL68_14665"
     CDS             complement(354..647)
                     /locus_tag="VEL68_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HXZ37261.1"
                     /translation="MTKPIDQFTWKDIPLGCVVAEAGNAREYKTGDWKSQRPVVDTSK
                     CIKCGVCWVFCPDAAIYKNEEGYFLANLDYCKGCGICARECWTGCITMVEEEK"
     gene            complement(765..1313)
                     /locus_tag="VEL68_14670"
     CDS             complement(765..1313)
                     /locus_tag="VEL68_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ37262.1"
                     /translation="MIEVRFHGRGGQGAVTSVELLALAAIEEGKFAQGFPSFGPERRG
                     APVVAFSRVNDHYIRLRSKIYTPDVAVVLDPSLLKIVDPSQGLKPGGLIIINTNKAID
                     QIRTDFGYQARLAAVDADRIAREELGLPIVNTTMLGALIRGTGIVALDSLAPPLKARF
                     GRGAERNLKALRRAYDETTLSS"
     gene            1526..2014
                     /gene="rfaE2"
                     /locus_tag="VEL68_14675"
     CDS             1526..2014
                     /gene="rfaE2"
                     /locus_tag="VEL68_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HXZ37263.1"
                     /translation="MNNKKIKNINAIKSIVARLKIRRKKVVFTNGCFDILHVGHIRYL
                     RKARSLGDILVVGLNTDRSVRQIKGEKRPIVPQGERAEVLAALEFVDYVVLFDEPDPF
                     VLIEKVKPTILVKGADWPKDKIIGRNVVEKAGGRVVRIPLVRGASSTGVIKKILQVYG
                     RG"
     gene            2159..3085
                     /locus_tag="VEL68_14680"
     CDS             2159..3085
                     /locus_tag="VEL68_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXZ37264.1"
                     /translation="MSKAIFTAAITGSIHTPTMTPYLPITPDQIADNAFQAYEAGAAV
                     CHVHARNPENGMPSPDIETFKKIITKIKSKCDIVVCTTTGGGLGMTPEQRVAIVSTCK
                     PELASCNMGSMNFALFPLLEKMKDFKYPWEKQYLEFTEDLIFPNTFKSLRVFLNLFNE
                     SQTKPELEVYDTGMINNAAFMIDRGLLKTPIYIQFVLGILGGIQATVENLVFLYESAR
                     RTIGEKNFVWSVCAAGRNQMPMCTHALLMGGNARVGLEDAVWLEKGVLAKNNGEQVAK
                     IIRIAKEFGIEPATPAEARKMLGLKGLDKVGY"
     gene            3324..4679
                     /locus_tag="VEL68_14685"
     CDS             3324..4679
                     /locus_tag="VEL68_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXZ37265.1"
                     /translation="MKKISLFHLIGIIYFTVSGGAFGLEELVSSTGPGLALLLLIVTP
                     LFWSLPVALMVAEMSSMLPLPGGYYRWVYFGLGRFWGFQEGWWTWLYTFVDMALYPVL
                     FADYARYLFPGLTGWQHWLTALLVIYSSLIMNLLGARLVGRSAVLAFVVVTLPFFLFA
                     MLGLGRIQNLPWNPWSPGGQSLVQTLGLGLAVVVWNYSGWDNVSTFAGEVDRPKRNYP
                     LALFASVPLISLLYLLPVGIALATTQNWHEWKTGDFPKIVAEVVGPWLGVIMSLAVMF
                     STWSLFNSQLLYTSRIPFAMAEDGLFPRWITRRNRRWGTPFASLLLCALIYSFFTLLD
                     FRKLVVIDVLVYSAALLLEMLALFKLRLTRPDLPRPFRIPGGWPVLLLVTLSMGVFIS
                     ACFIFSLLGENGGWIQAVIAVGMLLTGLLVYWISENFRKDSPPPAEWCRSLGSADRPS
                     G"
BASE COUNT          999 a         1392 c         1358 g         1116 t
ORIGIN      
        1 ccaaatgctc aggggggctg acagcgcccg gttggccacg gacatgacga tgggcagacg
       61 catgcccgcg gtgacgaaga ggatctcatg catcagttcc agcccctggg cgctggtgga
      121 ggtgtaggtg cgggccccta cggccgaagt tccagcgcag acggatagag cggtatgttc
      181 cgactccacc gggacaaact ccgcatccag ctctccattg gccaccagct cggagaggtg
      241 ctccacgatg tgggtttggg gggtgatcgg ataggcggaa atggcttccg cccgggccag
      301 cttgaccgcc tccgcggcag cgatggaaac ttccattcct acgcgtttat ccgtcatttc
      361 tcttcctcca ccatggtgat gcagcccgtc cagcattccc gggcgcatat gccgcacccc
      421 ttgcaataat ccaggttcgc caggaaatac ccttcctcat ttttatagat ggccgcatcc
      481 gggcagaaaa cccagcagac cccgcatttg atgcacttgg aggtgtccac caccggacgc
      541 tgggacttcc agtcgccggt cttatattcc cgggcgtttc cggcctcggc caccacgcat
      601 ccgaggggga tgtccttcca ggtgaattga tcgatcggtt tggtcacggt ttttcctccg
      661 ctcctttggg aaacattctt ctaaagcgcg cactcctctc cgccctaatg atcttttcct
      721 cctctgccgg ggagggaaat tgaggagagg gatagaggct tttttcagct gctcagggtc
      781 gtctcgtcgt aggctcgacg gagtgctttg aggttccttt ccgctcctct gccgaaacgg
      841 gctttcagcg gcggggccag ggaatccagg gccacaatgc cggttccccg gatgagggcg
      901 cctagcatgg tcgtgttgac gatgggaaga cccaactctt cacgggcgat ccggtcggcg
      961 tccacggcag ccaagcgggc ctggtacccg aagtcggttc gaatctggtc gatggcttta
     1021 ttggtgttga taatgatcaa gccgccgggt ttcagcccct gcgaggggtc cacgattttc
     1081 agcaggctgg ggtccaggac tacggccaca tcgggggtgt aaatcttgga cctaaggcgg
     1141 atataatgat cgttaacccg ggaaaaagcg acaaccgggg cgccccttcg ttccggccca
     1201 aaactgggaa atccttgagc gaatttccct tcttcgatgg ccgccagagc gagaagttcg
     1261 accgaggtaa ccgccccttg ccccccgcgc ccatgaaaac gaacctcaat catgattata
     1321 ttttcctcga tttcccttta ttttttaatg accgggatca tggataaaag gataccgccg
     1381 atcatctttc acatagcata ccccccttaa aagtgtcaac cctaattatg aatgaacctt
     1441 cagccagcaa gaaaatggaa accgcggagt tgcaaacccg tgtggcagat ggtaatattc
     1501 ccccatctcc cccggaggag ttgccatgaa taataaaaaa attaagaata tcaatgcgat
     1561 aaaatccatt gtcgcccgtt taaagatccg tcggaaaaag gtcgtattta ccaatggctg
     1621 ctttgacatt ctccatgtcg ggcatatacg ttacttgagg aaagccagaa gcctcgggga
     1681 catcctcgtg gttgggctaa ataccgaccg gtccgtcaga cagatcaagg gggaaaaaag
     1741 gcccattgtg ccccaagggg aaagagcgga ggtcttagca gccctggagt tcgtcgatta
     1801 cgtggtcctc ttcgacgagc cggacccctt tgtccttatc gagaaggtaa agccaaccat
     1861 cctggtgaag ggggcggatt ggcccaagga caaaatcatc ggccggaatg ttgtggaaaa
     1921 agctggcggc cgggtggttc gaatcccgct ggtgcgcggt gcttcgagta ccggagtcat
     1981 caaaaaaatt ctccaggttt atggccgggg ataaatgcaa accatccgga ggcggccgat
     2041 ctttggttcg gttgaagaaa atggccatct atgatagaag aaggacggag aggattctta
     2101 aagaagagat gaatcccctc tccggcaaat aaaaccccca ataaaggagg gaccattcat
     2161 gagcaaagcg atattcactg cggccatcac gggttccatc cacaccccga ccatgacccc
     2221 gtacctgccc attaccccgg accagattgc ggacaacgcc ttccaggcct acgaggccgg
     2281 ggcggcggtc tgtcacgtgc acgcccgcaa cccggagaac ggcatgcctt cccccgacat
     2341 agagaccttc aaaaagatca tcactaagat caagagcaag tgcgatatag tggtctgcac
     2401 caccaccggc gggggcctgg gcatgacccc tgagcagcgg gtggcgatcg tctccacctg
     2461 caagcccgaa ctggcctcct gcaacatggg ctccatgaac ttcgctctct tccccctctt
     2521 ggagaagatg aaagatttca aatacccctg ggagaaacaa tacctggaat tcaccgagga
     2581 cctgattttc cccaatacct tcaaatccct gcgggtcttc ctgaacctct tcaatgagag
     2641 ccagaccaag cccgagctgg aagtttacga tacgggaatg atcaacaacg ctgcctttat
     2701 gatcgaccgg ggtctgctga agaccccgat ctacattcag tttgtcctgg gaatcctggg
     2761 gggcattcag gcgaccgtcg aaaacctggt cttcctctat gaatcggctc gccggaccat
     2821 cggggaaaag aatttcgtct ggtcggtatg cgccgcagga agaaaccaaa tgcccatgtg
     2881 cacccatgcc ctgctcatgg gcggcaacgc gcgggttggc ctggaggacg ccgtctggct
     2941 ggaaaaaggc gtcttggcca aaaacaacgg ggagcaggtc gccaagatca tccgcattgc
     3001 caaggaattc ggcatcgagc cggcgactcc ggcagaagcc cggaagatgc tggggctgaa
     3061 agggttggac aaggtgggct attaggcgct aagagcgaaa ttgcaaccaa catgtgggta
     3121 aaagggggcc agaatgcagg ggcggttcgc gaaccgcccc tgtgcagtgc cgaccctgga
     3181 gggggccatt tcttccgcgc gcggtccggg aatattctaa ggatctttat tcctaaaaag
     3241 ggcaggtttc tgaagggaag cctgcccttt tttgtgggga ttctggtgcc cctgattttg
     3301 cccattttcc ttgctctacg aagatgaaaa aaatatccct tttccatctc atcggtatta
     3361 tttattttac ggtgagcggc ggggctttcg gcctggaaga actggtgagc tccacggggc
     3421 ccgggctggc cctcctgctg cttattgtga ctcctctctt ttggagtctc cccgtggccc
     3481 tgatggtggc cgagatgagt tccatgctcc cccttcccgg cggatattac cgctgggtgt
     3541 acttcggcct gggacggttc tggggatttc aagaggggtg gtggacctgg ctctatacct
     3601 ttgtggatat ggctctttac ccagtcctct tcgctgatta cgcccgatat ctgttccccg
     3661 gccttaccgg atggcagcac tggctcacgg ccttgttggt gatctactcc tccctgatca
     3721 tgaatctgct cggagcccgc ctggtgggaa ggagcgcggt cctggctttc gtcgtggtga
     3781 ctctcccctt ttttctattt gctatgctgg ggctcggcag aattcagaac ctcccctgga
     3841 atccttggag cccggggggg caaagtctcg tccagaccct cggcctgggc cttgcggtcg
     3901 tcgtctggaa ctactccgga tgggacaacg tgagcacttt tgccggagaa gtggaccggc
     3961 ccaaacgaaa ttacccgctg gccctgttcg cttccgtgcc cttgattagc ctcctttacc
     4021 tcctgccggt cgggatagcc ctggccacca cgcagaactg gcacgagtgg aagaccggtg
     4081 attttcccaa gatcgtcgcc gaagtggtag ggccctggct tggggtaatc atgtctctcg
     4141 cggtgatgtt cagcacgtgg agcctgttca acagccagct tctctacact tcccgaatcc
     4201 cctttgccat ggcggaggac ggtctttttc cccgctggat cacccggcgg aaccggcgct
     4261 gggggactcc tttcgcttcc cttcttctgt gtgcccttat ctactccttt ttcacccttc
     4321 tcgatttccg gaagttggta gtcatagacg tcttggtcta ttccgccgcc ctgctgctgg
     4381 aaatgctggc tttgttcaag ctgcgcctca cgagacccga tctcccccgt cctttccgta
     4441 ttccaggcgg ctggccggtc cttctactgg tcacgctttc catgggcgtt ttcatatcgg
     4501 cctgttttat cttctcgctg ctgggagaaa acgggggctg gattcaggcg gtcatcgcgg
     4561 tagggatgct gcttaccggt ctcctggttt actggatttc ggaaaatttt cgcaaagact
     4621 ctccgccgcc cgcagagtgg tgccggtccc tgggttcggc ggatcgccct tccgggtgaa
     4681 agtcgaccgg ctgtccttag atggaattct gcatcgcctt tccggcaatg gtccggtgca
     4741 gctcttccct tttctggaag atcagctgga tatcgatttc acagacctcg cagaccctct
     4801 gggtgatgtc atcgaccccc gggggacaac cggggacgaa aggaaaccgc ccttccattt
     4861 tccgc
//
LOCUS       DASPRR010000402        11809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1386840, whole genome shotgun sequence.
ACCESSION   DASPRR010000402 DASPRR010000000
VERSION     DASPRR010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386840"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..975
                     /locus_tag="VEL68_14690"
     CDS             418..975
                     /locus_tag="VEL68_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37266.1"
                     /translation="MEEVGDRRRRIIVDIDNTLWYLAPELWEGLKKVNPEMPPPAEWD
                     SRRSIERFMPIKEFFRVLKGIHMRQEIYPPFPDAEYFLGGLKERGFYIVIASHRAPDT
                     YEPTVNWMRKYRLAHDEIHLSYDKSVLFPESIALVDDSPLNLGKAVEAGILGTGLLFP
                     WNENSGFPLFKTLSEVLGYLELKLG"
     gene            complement(1362..2312)
                     /locus_tag="VEL68_14695"
     CDS             complement(1362..2312)
                     /locus_tag="VEL68_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HXZ37267.1"
                     /translation="MSSLVPTGPKILPEFKGLIFDIQKFCVHDGPGIRTVVFFKGCNL
                     RCFWCQNPESISPGPEVAHSKDRCLQCGACLKACAHKAIVSSPGGFVRHPSLCQKCMA
                     CVQTCPTQALRPVGQWMTVDAVLEKVAEDSPFYENSGGGVTFSGGEPTLQFHFLKTIL
                     EESGRRHFHRVLETNGRLSWKRLEQLLPHLELVLFDLKHLHGRTHEQYTGSGNRLIRM
                     NLKRLAKESRVPWIPRVPLIPGLNDDPKHLAHLGGWVKGLGAREIHLLPYHRLGEPKM
                     QELGYLRRSSMAGVRPPSEEEMENAGQVLKREGLSVIIGG"
     gene            complement(2309..4789)
                     /locus_tag="VEL68_14700"
     CDS             complement(2309..4789)
                     /locus_tag="VEL68_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HXZ37268.1"
                     /translation="MRQIQTERSRITKGSRSYNAKYNDVINASPRIQRLRERFFTYRP
                     SICIERARIVYTYYSNPQNQSLPLILQRAGAFRAVLNQVPIRIYPDELLVGSLASRPR
                     AYPMFPENFGDLHSKELETISNRSPDPFEVSEADKKELQEKIFPLWRGKSADELFSSV
                     LSPEEWQLIFRDPEDLSKGTGIISLYAALLGTGGHSTLDYAKLLKKGFRGIREEALAR
                     MQEVDHFSGPDIEKALFYRAVVDCCEGIIEFGLRFSRLAAEMASKEPDPERRQELEAI
                     SQVCSQVPGSPARSFHEALQSVWFAYLAILQEDYNRCNPLGRIDSYLYPFYQSDRDEG
                     RLTVEQAQDLLDCLWMKLGETNHVTWGLSAKITAGFPVQQQIPVGGLTPEGKDATNPL
                     TYQCIQASMNTRLHQPSITVRLHKESPMDLYYKAAKLACLGTGHPSFFNDEVVVPALV
                     KNGIAPEHARDYSSVGCVGAQVSGCGKGSHNAGYLNAAAALEFTLTNGYWRYGQKQVS
                     IQTGDPREFTSFDQFWDAFECQFRYMIRAHLSASLKVEYLHAQHNPTPYLSSLTQGCL
                     ESGRDKTRGGAIYNLAISFRAVGLADVADSLIAVKKLIFEEKLISMDRLLQALDVNFE
                     GHEALRQMLINRSPHYGNDDGYADEMARRVVDVLTDEGRRHKSYFGGDFQLGFGSVSG
                     HWPFGVVLGAFPDGRKAGEPLADGIGPVHNRDKNSPTGVLKSVGKMDHIGLSGGSILN
                     LKFPPQVVEGEKGLANFVSFLKSFVQLKAWHCQFNIVDANLLREAQRNPEKYQDLLVR
                     VAGYSAYFTGLSKELQDDIVDRTEHLLQ"
     gene            complement(5022..5399)
                     /locus_tag="VEL68_14705"
     CDS             complement(5022..5399)
                     /locus_tag="VEL68_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ37269.1"
                     /translation="MSPLQRPLSSRFYTEIFGAGVVFDDILAGVRNVLMRIGTGPINF
                     YDQPPRNFGRTAVDHLGIYTDDISALVVHMEAKGFKFRTPIRVFGDLKYIMLEGPERV
                     PIEVVENKAAWYRVPGAEIIDSK"
     gene            complement(5569..6273)
                     /locus_tag="VEL68_14710"
     CDS             complement(5569..6273)
                     /locus_tag="VEL68_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37270.1"
                     /translation="MLSVHNVAVGYRGLLAVQEVSFEVKKGEVVSLIGSNGAGKSTII
                     KTIAGLLHPQSGEIFFENSPIHRIPAYEIVKKRISMIPEGRRLFGRLSLRDNLELGAY
                     ALSAREEIEETLKTVFSLFPILAERKSQRADTLSGGEQQQLAIARGLMSRPSLLMLDE
                     PSLGLMPKLVSEIFKIIREISQQGVTVLLVEQNVFEALQVSNRAYVLQTGRIVLEGKG
                     EDLIKSDLVRKAYLGL"
     gene            complement(6257..6997)
                     /locus_tag="VEL68_14715"
     CDS             complement(6257..6997)
                     /locus_tag="VEL68_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37271.1"
                     /translation="MKSPLFLEVQRVSKRFGGLSANENIDFAIKEGEIVSIIGPNGAG
                     KTTLFNCITGFYHPNSGKVFFRGEDITRHRADAICKMGIARTFQIVQVISDMTVLENV
                     ATAGLLRYTRVAHAAEKAEEVLAFTGLIEKKEALGTDLTMADKKRLEVSMALATRPVL
                     LMLDESMAGLTRVELRGMIDLIKKIRRNGLTLVIVEHVMEAVMEISDRVIVLNSGKKI
                     MEGAPQEVVQNKEVIQAYLGEKYSAVSP"
     gene            complement(6994..7959)
                     /locus_tag="VEL68_14720"
     CDS             complement(6994..7959)
                     /locus_tag="VEL68_14720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37272.1"
                     /translation="MGIFKWKQVYLGITALILLIFFPLAFPKPFPQHVMILVFMFGMM
                     GAAWNIMGGYAGMFSFGQVAFFGIGAYTSSFLLITYQVNPWVGLIIGGLIAALVGAAI
                     GYPCSNLRGHYFAIASIAFGEIVRTLFTNWKLIGAAEGLTLPMLDESFQNFIFHSSKL
                     PYYYIMLVFLLLSLLVCYFVATSKMGFYFRAIKESHDVAKGLGINVVWYRLVAIMISA
                     FLTAMAGTFYAQYILYLDPDSVLVLPISVQIVLISMLGGAGSVLGPVIGAVILMPISE
                     VTRVMLGHKGTGIDMLIYGALITFISVYQPKGVWGLFTGMAKRSK"
     gene            complement(7963..8823)
                     /locus_tag="VEL68_14725"
     CDS             complement(7963..8823)
                     /locus_tag="VEL68_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37273.1"
                     /translation="MPEQLGQQIVNGLLIGFIYSLIAIGLTLIWGVMNIVNFAHGDFL
                     MLSMFASFWLYTLYGLDPIFSIPICTALLFVLGLLIYRFIVSKVMKGPMLAQLVVTFG
                     VSIFLANLAVFLWTPDFRLIEKPLLSGTWAIGEIKVSVPKFVATIGSVIVSLFVFLFL
                     KKTKTGKAILATEMDREAALLMGINTDRINSISFGLGAALVGVAGAFLSTYYYIYPQV
                     GGTFGLIAFSIVALGGFGSIEGAFIAGIIVGVAQTLGGFVFDPAYKYAMVFLIYLIVV
                     WIRPQGLLGW"
     gene            complement(8888..10147)
                     /locus_tag="VEL68_14730"
     CDS             complement(8888..10147)
                     /locus_tag="VEL68_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37274.1"
                     /translation="MGTKRLFAVYLGLALLLCLMGSSSAQQRVVKIGAIYPLTGNLAT
                     TGLDCKRGVEMAVEIINGKYDLSLPLAKTEGLPDLGGAKIEVIFGDSKGDPRNGMAEA
                     ERLVTQEKVVAVIGAYQSAVTKTASQATERLKIPFVNSDSTSPTLTERGFKYFFRPEC
                     TDTIFARNQFEFLQDIKKKTGMKLESVALLYENTEWGANVGKEEQKYAKEFQYKILED
                     IPYSQNATDVSSEVGKLIKGKPDVLLHASYITDAILYTKTFKELGFAPKGLLTMAGYL
                     EPDYLKTVKADGNYMVVRSTFALDLAKRKPLVAQVNDLFKKKFGMDMSGNAARSFTAP
                     FVVAEAINRAKSTDPEPLTKALTETNIPGDQIINPWKGIKFDPKTHQNIYATGILVQT
                     QNEQYFTVWPFDMASKDVIWPFPGWKK"
     gene            complement(10455..11180)
                     /locus_tag="VEL68_14735"
     CDS             complement(10455..11180)
                     /locus_tag="VEL68_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37275.1"
                     /translation="MILSIEDLHVAYGPIEALHGITFHVARGEIVTLIGANGAGKSTT
                     LMSIMRLPPPEAPLYRSGQMIFQEQDLTHFSAHDVVSRLGVSLVPEGRRIFGNLTVWE
                     NLKLAAYAHEDRQKMQEKANWIFGLFPRLKERRSQMGDTLSGGEQQMLAVARAMMTGG
                     TMILLDEPSMGLAPFLMYELFRVLKEINQQGTTILLVEQNARIALKYAHRGYVLETGR
                     IILEGPAKDLAQREEIKKAYLGE"
     gene            complement(11180..>11809)
                     /locus_tag="VEL68_14740"
     CDS             complement(11180..>11809)
                     /locus_tag="VEL68_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37276.1"
                     /translation="NLVCGVYRPSQGEVNMDGRNLVGFYPHQITASGIARTFQNIRLW
                     PEMTVLDNLRVAHNYHLGYGILDVVLQTSRHRKAEAGIHNRALETLEIMGLRDHGREL
                     AKNLPYGLQRKVEIARALMIKPKLLLLDEPSAGMNLGEKHGLIELIRFIRKQFHLTIW
                     LIEHEMRVVMTLCEYIHVLDFGATIAGGTPEEIQRNPKVIEAYLGKEVD"
BASE COUNT         2842 a         3189 c         3103 g         2675 t
ORIGIN      
        1 cgaaggctcg ttcggataga gcgccgccca tttggacgtc caatctccca cgttcatggt
       61 tccctccctg gtcaatacag gttttgaact cgaaccacag tggatgcgga aaaatcttgg
      121 attgaaacga gataaaaatc ctgccagagc gtattgccgg caaaggtcag ataggttaaa
      181 gcgcagtctg aaaatcgctg aaaaattaaa accttgtcga aatggccggt ggaaatgaat
      241 gaaatagaat cgcgttttga cccgaactgg gaagacggca taatattatt tgaattcgag
      301 gaaaatatca aagaaaatat ttatgaccta acggattgag ttggacgctg agcattcgaa
      361 ttttataaga ccaggcggct gcgcacccgt gcgggattct gaggaaaggc ggttccgatg
      421 gaagaagttg gagatcggcg gcgaagaatc atcgtagaca tcgacaatac cctctggtat
      481 ctagcgccgg agctgtggga ggggcttaaa aaggtgaacc cggaaatgcc cccacccgca
      541 gaatgggatt cccgaagatc gatagaacgg ttcatgccca tcaaggaatt cttccgggtc
      601 ctgaaaggca tccatatgcg gcaggagatt tacccaccct tccccgatgc ggaatatttc
      661 ctcggtggcc tcaaagaaag gggtttctac atagtcatcg ccagccaccg agcccccgat
      721 acttatgagc ccacggtcaa ctggatgcga aagtaccgcc tggcccatga cgaaattcac
      781 ctcagctatg ataaatccgt cctcttcccg gagagcatcg ccctggttga tgacagccca
      841 ctcaatctag gcaaagccgt agaggcggga atcctcggaa ccggccttct cttcccctgg
      901 aatgaaaatt ctgggtttcc tcttttcaaa actctttctg aggttctagg atatctggag
      961 ttgaagttgg gttagcccga tggaagctgt cgcagtccgc taaaaaaaga ttgtacgcaa
     1021 gactctaaga aaaattgtgg gggcaacccg gcttggctac catcatgcgg gcggccacat
     1081 ggggccgccc ctaaaagaat ttaaaggcaa aaagatccta acgggaacaa aatcgatcgc
     1141 agcgaaaatc caaaaagttc tccgtcccct ctccttaagt ttcgtttaaa aggcatatat
     1201 gaaaatcata tcggaactgt tttcagagag agaaaaaagc ggatacggat atgtgcattt
     1261 ttcaaaacaa atgcctacac ctgcaaatgc tcagcggagg gccgcttaga aggccgagca
     1321 ttcctgtcag tgatggtcga tcccggtcca atataggaga atcatccccc gataatcacc
     1381 gacaatcctt ccctcttcaa cacttggccg gcattttcca tctcttcttc ggaaggaggc
     1441 ctgacacccg ccatcgaact ccggcggaga tagccaagtt cctgcatctt gggctctccc
     1501 agtcggtgat agggaaggag gtgaatctcc cttgcaccca gccccttcac ccatccgccc
     1561 aaatgggcca gatgttttgg gtcatcattc aatcccggga ttagagggac gcgggggatc
     1621 caaggaaccc tgctttcctt ggccagtcgt ttcaagttca tccggatcaa gcggttcccg
     1681 gatccggtat actgctcatg ggtccggcca tgcaggtgtt tcaggtcaaa gagaacgagt
     1741 tccagatggg gaagcagttg ctccagcctt ttccaggaga ggcggccgtt ggtctcgagg
     1801 acccgatgaa aatggcggcg gccggactcc tccagtattg ttttgaggaa atgaaattga
     1861 agcgtgggtt ctccccccga gaaggtcacg cccccgccgg aattctcata gaacggggaa
     1921 tcttcggcca ctttttccag gaccgcgtcc accgtcatcc attggcccac ggggcggagg
     1981 gcctgagtgg gacaggtctg aacgcaggcc atacattttt ggcaaaggct ggggtgccgg
     2041 acaaaacccc cgggtgatga tacgatagcc ttatgggcac aggccttgag gcaggcgccg
     2101 cattggagac accggtcctt tgaatgagcg acttccggcc ccggggagat agattcgggg
     2161 ttttggcacc agaaacatcg caagttgcat cctttgaaaa atacaaccgt gcgaattccc
     2221 ggtccatcgt ggacacagaa tttttgaatg tcgaaaatga ggcccttaaa ctccggcaat
     2281 atctttgggc cagtcgggac caaggagctc attgcaggag atgctcggtc cggtcgacga
     2341 tgtcatcttg cagttctttg gacaggccag tgaaataggc actatatccg gccacccgta
     2401 cgaggaggtc ctggtacttc tccggattcc gctgggcctc ccggagaagg tttgcatcca
     2461 cgatgttgaa ctgacagtgc catgccttca attggacaaa gcttttcagg aagctgacga
     2521 aattggccag ccccttttcc ccctccacca cctggggggg gaatttaaga ttcaggatgg
     2581 agcccccgga gaggccaata tgatccatct tccccacgga tttcagcaca cccgtagggc
     2641 tgttcttgtc ccggttgtgc actgggccga tcccgtccgc caaaggttcc cccgctttcc
     2701 ggccgtccgg aaaagcgccc aaaacgaccc cgaaaggcca atggccggaa acggaaccga
     2761 aacccagttg aaaatctccg ccaaaatagc ttttatgccg ccgaccttca tccgttaaga
     2821 catccaccac cctgcgagcc atttcatcgg catacccatc atcgtttccg taatggggag
     2881 agcggttgat cagcatctgg cgcagggctt catgcccttc gaagttgacg tcaagggcct
     2941 gtagcaaccg gtccatagag atcaactttt cctcgaaaat caatttcttg accgcgatca
     3001 aagaatcggc cacatcggcc aggcccacgg cccggaagga gattgccagg ttatagatcg
     3061 cccctcccct cgttttgtcc cgcccgctct cgaggcaacc ctgggtcagg ctgctgagat
     3121 aaggggtcgg gttatgctgg gcatggaggt actccacctt gagcgaggcg ctcaggtgcg
     3181 cgcgaatcat gtaccggaac tggcattcga aggcatccca aaactgatcg aaggaagtga
     3241 actcccgggg gtctccagtc tggatggaga cctgcttttg gccgtagcgc caatatccgt
     3301 tggtaagggt gaactccaga gcggcagcgg cattcaggta accggcattg tgggatccct
     3361 tcccgcaccc ggaaacctgg gctcctacgc atcccacgga gctgtaatcc ctggcatgct
     3421 ccggggcaat gccgttcttc accagagcgg gcaccactac ttcgtcgtta aaaaacgagg
     3481 gatgacccgt gcccaggcat gccagcttgg cagccttata ataaaggtcc atgggggatt
     3541 ctttgtgcag gcgaacggtg atactcggct ggtggagtcg ggtgttcata gaggcctgga
     3601 tacattggta ggtcaggggg ttggtcgcat ctttcccctc gggtgtcagc ccacccaccg
     3661 ggatctgctg ctggaccgga aagccggcag ttatcttggc ggataaaccc caggtgacgt
     3721 ggttggtctc ccccagcttc atccacaggc agtccagtaa atcctgggcc tgctccacgg
     3781 tcaatcggcc ttcatcgcga tcgctctggt agaagggata gagataggaa tcaatccttc
     3841 ccagcgggtt gcatcggttg taatcctctt ggaggatggc cagataggca aaccacacag
     3901 attgcagcgc ctcgtggaaa ctgcgggccg gggaaccagg aacctgggag cagacctggc
     3961 tgatggcttc cagttcctgc cggcgctctg ggtcgggttc tttggaagcc atctccgccg
     4021 ctaaacggga gaagcgcaag ccaaattcga tgatcccctc acagcaatcg accaccgctc
     4081 ggtagaaaag ggccttttca atatccgggc cggaaaaatg atcgacttcc tgcattctgg
     4141 cgagcgcctc ctccctgatt ccccgaaatc ccttttttaa gagcttggca taatcgaggg
     4201 tgctgtgccc gccggtgccc agaagtgccg cataaaggct tatgatcccg gtgcctttac
     4261 tcaggtcttc cgggtcgcgg aagatcaact gccattcttc gggtgaaaga accgaagaaa
     4321 aaagctcgtc ggccgatttt cctctccaga gggggaaaat cttttcctgc agttctttct
     4381 tatccgcctc ggatacctcg aagggatccg gggaccggtt ggagatcgtc tccaattctt
     4441 tactgtgaag gtcgccgaag ttttccggga acatcgggta ggcccgggga cgggaggcca
     4501 gggagcccac tagcaattca tccgggtaga tcctgatggg aacctgattg agcacagccc
     4561 gaaacgcccc agccctctga aggatgaggg gcaaggattg gttctgggga ttggagtagt
     4621 acgtatatac aatccgcgct cgttcgatgc agatggaagg ccggtaggta aaaaaacgtt
     4681 cccgcaaccg ctggatccgg ggggaagcat taatgacatc gttatatttg gcgttgtaac
     4741 tgcggctccc cttcgtaatc cttgaacgct ccgtttgaat ttgccgcatt tccccctccc
     4801 ttctttggct cgtgcgggca tccaaacacc cgcctgacca ttcgaattgg acctggcgga
     4861 aaagcatatt aagaagaccg caaggaataa taatatacca gaaaattttc cctgaaagaa
     4921 ggccgtttct aatggtctct atatcgaaat tttttcgacg attgggagac aaaggatagc
     4981 aataaacgaa ctgagggacc tttttcaata acgatcacag ctcacttgct atcgataatt
     5041 tctgcacctg gaacacggta ccaagctgcc ttattttcga caacctcaat cggcactcgt
     5101 tcagggcctt ccagcatgat gtatttgagg tccccgaaaa ccctgattgg cgttcggaac
     5161 ttgaagccct tcgcctccat atgaacgacc agtgcggaaa tatcatccgt gtagatgcca
     5221 agatgatcca cggcggttcg cccgaaattc cggggaggct ggtcgtaaaa attgattggg
     5281 ccggttccta tgcgcatcag tacattccgc accccggcaa gaatatcatc gaagacgacc
     5341 ccggcgccga atatttcggt atagaaccga gaggaaagag gtctttgtaa tggagacaaa
     5401 tagagcggga cgcaggaaag atcagcaaat taaaaaaata aaccctaagg gaatataggc
     5461 atgcttttgg tctttctggc ctatcatcac gaacgattca gagaagtgct gagggatggt
     5521 tggttttata gttagaagaa aagttgatgg gcaccggtca ttcctagatc acagacccag
     5581 ataggctttt ctgacgaggt ccgattttat gaggtcttct ccttttcctt ccaaaacgat
     5641 gcgtccggtt tgcagcacgt aggcccggtt ggagacctgc aaagcctcaa agacgttttg
     5701 ctcaaccagc aaaacggtca ccccctgctg gctgatttcg cggatgattt tgaagatttc
     5761 agaaacgagc ttgggcatca atcccagaga aggttcatcc agcatgagca gagagggacg
     5821 ggacattaat cctcgggcaa tcgccagttg ctgttgctct cctccgctta aggtatcggc
     5881 gcgctggctc tttctttcgg ccagtatggg aaagaggcta aaaacggttt tcagggtctc
     5941 ttcgatctct tccctggcgg aaagcgcgta cgcgcccaac tccaggttgt cccgcaaaga
     6001 taatcggcca aaaagccttc tcccttccgg gatcatggaa attctttttt tcacgatttc
     6061 ataagctggg atccggtgga tggggctgtt ctcgaaaaag atttcaccgc tctggggatg
     6121 gagaaggccg gcgatcgttt taatgatagt agactttccg gcgccgttgg aaccgatgag
     6181 gctgaccact tccccttttt taacctcaaa ggagacttcc tgcacggcca ggagccctct
     6241 atagccgacg gcgacgttat ggactgacag cactgtactt ttctcctagg taagcttgaa
     6301 tgacttcctt attctggacg acttcctgag gggctccttc catgatcttc tttccggaat
     6361 tgaggacgat cacccggtcc gatatttcca tgaccgcttc cataacgtgt tccacgatga
     6421 ccagggtcaa gccattcctc cgtatctttt ttattaaatc aatcatccct cgcaactcga
     6481 ctctcgttaa gcccgccatg gattcatcga gcattaataa taccggccgg gtagcgagag
     6541 ccatggaaac ctccaatctc ttcttgtcgg ccatggtcag atcggttccc agggcctctt
     6601 tcttttctat gagccctgtg aaggccaata cctcctcggc tttttcggcg gcgtgggcta
     6661 ctcgggtata ccggagcaaa cccgctgtgg ccacattctc aaggaccgtc atgtcgctga
     6721 tgacctgaac gatttgaaag gtgcgggcaa tccccatttt acatatcgca tctgcccggt
     6781 ggcgggtaat atcctctcct ctgaaaaaga ccttcccgct gttggggtga taaaacccgg
     6841 taatacagtt gaagagggtc gtcttccccg cgccgttggg cccgataatg ctgacgatct
     6901 ctccctcctt gatggcaaaa tctatattct cattggccga gagcccgcca aaccgcttgc
     6961 ttaccctttg gacctctaaa aagagaggac tcttcatttt gacctcttgg ccataccggt
     7021 aaacaagccc caaactccct tgggctggta aaccgagatg aaggtgatta aagccccgta
     7081 gatcagcatg tcgattcccg tacctttatg gcccagcatc acccgggtta cttcggagat
     7141 cggcatcagg atgacggccc cgatcaccgg tcccaagaca gaaccggcgc cccccagcat
     7201 ggagatgagc acgatctgca cggagatggg caggaccagg acactatccg ggtcgaggta
     7261 caggatgtac tgggcataaa aggttccggc catcgccgtc aaaaaggcgc tgatcatgat
     7321 ggcgaccaga cggtaccaaa ccacgttgat gcccaggccc ttggccacgt cgtgactctc
     7381 tttgatggcc cggaaataga agcccatctt ggaagtggcc acgaaatagc agaccagaag
     7441 ggatagaagc aggaaaacca gcataatgta gtagtagggc agcttggaag aatggaaaat
     7501 aaagttctgg aaggattcat cgagcatcgg cagggtcaag ccttcagcgg caccgatgag
     7561 cttccagtta gtgaaaaggg tgcgcacgat ctctccgaag gcaatactgg caatggcaaa
     7621 gtagtggcct cgcaaattgg agcagggata cccgatcgct gctcccacca gggcggcgat
     7681 caggccgccg atgatcaacc ccacccatgg attgacctgg taggtgatca acaaaaagga
     7741 tgaggtgtaa gccccgatgc cgaaaaaagc tacctggccg aaggagaaca tcccggcata
     7801 gccacccatg atattccagg ccgcccccat catgccaaac atgaagacga ggatcatcac
     7861 atgctgggga aagggcttgg ggaaagccaa agggaagaag atcaaaagga tcaatgcggt
     7921 gatgccaaga tagacctgct tccacttgaa tattcccatg tcctaccacc ccagcagacc
     7981 ttgcggcctg atccagacaa caatgagata aatcaagaag accatggcat acttataagc
     8041 cgggtcgaag acgaaaccgc cgagggtctg ggcaacacca acgatgattc cagcgatgaa
     8101 agccccttca atacttccaa aacctcccag ggccacgatg gagaaggcga tcaggccaaa
     8161 ggtccctccc acttgaggat agatataata gtaggtggac aggaaagcgc cggccacccc
     8221 taccagcgca gcacctaacc caaaagagat ggagttgatc cggtccgtat tgatccccat
     8281 gagcagggcg gcttcgcggt ccatctccgt agccaggatg gccttgcccg tcttggtctt
     8341 tttcaagaag agaaagacaa agagggagac gattacgctg ccgatggtcg ctacgaattt
     8401 gggaacgctc accttaatct ctccgattgc ccaggtgccg gagagcagtg gcttttcaat
     8461 caggcgaaaa tcaggagtcc acaaaaagac ggccaggttg gccaggaaaa tagacacccc
     8521 aaaggtgacg accagctgag ccaacatggg gcccttcatc accttgctga cgatgaaacg
     8581 atagatcagc agccccagaa cgaagagcag ggccgtgcag atcgggatgg agaagatggg
     8641 atccaggccg tacagggtgt aaagccagaa ggatgcaaac atgctgagca tcaagaaatc
     8701 gccgtgggca aagttgacga tattcatcac cccccagatg agagtgagcc cgatggcaat
     8761 cagagaatag ataaaaccga tcaagagccc attgacgatt tgctgcccca gctgctccgg
     8821 cattctgcct ccctctcgca tgaatccaag gaggagccag agacccggct cctcctgcct
     8881 tgcccgtcta ttttttccaa ccggggaaag gccagatgac atcctttgag gccatgtcaa
     8941 aaggccagac cgtgaagtac tgctcattct gggtctgcac caggatccct gtggcgtaaa
     9001 tgttttggtg ggtcttgggg tcgaatttga tccccttcca ggggttgatg atctggtcgc
     9061 caggaatatt ggtctcggtc agagctttgg tcaagggttc ggggtcagtt gatttcgccc
     9121 ggttgatggc ttccgccacg acaaaaggtg cggtgaaact ccgggccgca tttccgctca
     9181 tatccatccc gaactttttc ttgaagagat cgttgacctg ggccacgaga ggttttcttt
     9241 tggccagatc gagagcaaag gtcgatcgaa cgaccatata atttccatcg gcctttacgg
     9301 ttttcaggta gtcgggttcc aggtatccgg ccatggtaag aaggcccttc ggcgcaaaac
     9361 ccagttcttt gaaagtcttc gtataaagga tggcatcggt gatataggat gcgtgcagga
     9421 gcacatcggg cttgcccttg atcagcttcc ccacttcgct ggagacatcg gttgcattct
     9481 gggaataggg gatatcttcc aggattttgt actggaattc cttggcatac ttctgctctt
     9541 ccttgcccac attggcgccc cattccgtgt tttcgtagag tagggccacc gactcaagct
     9601 tcatgccggt tttcttcttt atgtcctgca ggaactcaaa ctggtttcgg gcaaaaatcg
     9661 tgtccgtgca ttcgggccgg aaaaagtact tgaatcccct ttccgtcaat gttggcgaag
     9721 tcgaatccga gttcacaaag gggatcttaa gccgctcagt ggcctggctg gctgtcttgg
     9781 tcaccgcact ctggtaagct ccgatcaccg caacgacttt ttcctgggtg accagacgct
     9841 ctgcttcggc catcccgttt cgggggtccc ccttgctatc cccgaagatg acttctatct
     9901 tcgcccctcc caagtcgggc aggccttccg ttttcgccaa aggcagactc agatcatatt
     9961 ttccattgat gatttcgacg gccatctcaa cccctcgttt acaatccaac cctgtcgttg
    10021 cgagattacc cgtcagggga taaatggctc cgatcttgac taccctctgt tgagcagatg
    10081 acgagcccat caaacaaagg agtaaagcca aaccaaggta cacagcgaaa agtctcttcg
    10141 ttcccatttt gctctccttt cctttgccta ggttctttag tggatcagaa tatttcttgc
    10201 ggtccgggca atgagaaaaa aacggcgagg gaatatcaag acctccccgg agccattcct
    10261 aatggcattt tccagaatcc ctttctcacg ggccatccct gaaagttaag agctgggtta
    10321 ttgccttcat tgagtcacgg ggaaaaacga caaacacaaa ataagtctat cttggaggat
    10381 agtcaagggg aaaaaggatg cgccgcatcg ggggcaaacc cttcccgcaa caaccatcga
    10441 gtgtcgccac ccaatcattc cccaagatag gcctttttga tttcttctct ctgggccagg
    10501 tctttggccg gcccttccag gatgatccgg cccgtctcca ggacataccc gcgatgggcg
    10561 tattttaacg cgatgcgagc attctgctcc acgagcagga tcgtcgttcc ctgttggttg
    10621 atttccttca ggacgcgaaa aagttcatac atgaggaagg gcgccagccc catggaaggc
    10681 tcatccagaa gaatcatggt tccaccggtc atcatcgccc gggccacggc gagcatctgc
    10741 tgttctccgc cgctcagggt atcccccatt tggctacgcc tttccttcag ccgagggaag
    10801 agtccgaaaa tccagtttgc cttctcctgc atcttctgcc ggtcctcgtg tgcataggcg
    10861 gccaatttca agttttccca caccgtcaaa ttcccgaaga tcctccgccc ttcagggacc
    10921 agggagaccc ccaaacggct caccacgtcg tgagcggaaa aatgggtgag atcctgctcc
    10981 tgaaagatca tctgtccgga tcgatatagg ggagcctcgg gaggagggag gcgcataatg
    11041 ctcatcaggg tcgtcgattt tccagcgcca ttggccccga tcagggtcac gatttctccc
    11101 ctggccacat gaaaggttat tccatgcagg gcttcgatag ggccataggc tacgtgaagg
    11161 tcctcgatgg acagaatcat caatccacct ctttgcccag gtaggcctcg atcaccttcg
    11221 gattcctctg aatttcttcg ggtgttccgc cggcgatcgt tgctccgaaa tccaacacgt
    11281 gaatgtactc acagagggtc atcacgaccc gcatctcgtg ttcgataagc cagatggtca
    11341 ggtggaattg cttgcggatg aagcgaatca actcgatgag cccgtgcttt tcgcccaggt
    11401 tcattcccgc cgagggttcg tccaaaagga gcagtttcgg cttaatcatg agggcccggg
    11461 caatttccac cttgcgctgc agcccataag gcaggttctt tgccagctcc cggccgtggt
    11521 ctctgagacc catgatctcc agggtttcaa gggcacgatt atgaattccg gcttctgctt
    11581 tccggtgccg tgaagtctgc aagacaacat ccagaattcc atatcccaga tgatagttgt
    11641 gggcgacgcg taggttatcc aggacggtca tttcgggcca tagacggatg ttttgaaagg
    11701 tccgggcaat gccgctcgcg gtgatttgat gaggatagaa gcctaccagg ttccgcccgt
    11761 ccatgttcac ttccccctgg gatggacggt acacaccgca aacgaggtt
//
LOCUS       DASPRR010000403        18180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_240908, whole genome shotgun sequence.
ACCESSION   DASPRR010000403 DASPRR010000000
VERSION     DASPRR010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18180)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18180
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240908"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2238
                     /locus_tag="VEL68_14745"
     CDS             <1..2238
                     /locus_tag="VEL68_14745"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXZ37277.1"
                     /translation="IFAVDLKLPGMLHAKVLRSPIPHGRILRIDTEKAKNLPGVAAIV
                     TGKDFSSLFGATIGDQGFLARGKVRLVGDAVAAAAAVDADTAEEALSLIKVEYEPLPG
                     LFDPVDALKNREVLIHEDLGKYSVAPGIFPLPGTNICNHFRLRKGDMERGFKESDLVL
                     EDTYRSHGVQHAHLEPHAAIAQVDPSGRVQVWTNTQTPYFNRKAIAQALSLPLNKVRI
                     TGTAVGGGFGGKSYLKAEPVCVALALKTRGRPVKLVYTREEEFSIAPVRHPTIIRCKT
                     GMKRDGTWMGQETELIFDTGAYADIGPRVTRNAGFSATGPYKVPHVRIDSYCVYTNNS
                     IGGAFRGFGTPQVTWAIESHLDSMAEGLGIDPLELRLKNAVEEGSISATGQALYSVGL
                     KDTLRQAADKIGWGQGRTPFRGKGIACMHKSTVTPSASSAFVKLNEDKSVTLLVSTVE
                     MGQGASTLLAQIVAEELSVPVENVSVVSPDTDVTPYDMASVSSRSTFFAGNAVRRAAI
                     DAREQLLEIASEILEASPKDLVLERGRIFVRGVPDRAIPLADLPLGEAFYVGALGRGK
                     GRPVLGRGSYAVEDATALDRETGQGKNPSAFWMYATQAAEVEVNPRTGQVKVLRLTSA
                     HDVGRALHPVSIEGQIQGALVMGIGTTLFEDLDMREGKAQNHNFSSYKLPSALDIPEM
                     IPIIVEAPDRNGPYGAKGLGEPALSPTAASIANAIHAATGVRVKDLPITPEKILAGLV
                     QKENP"
     gene            2235..3902
                     /gene="ilvD"
                     /locus_tag="VEL68_14750"
     CDS             2235..3902
                     /gene="ilvD"
                     /locus_tag="VEL68_14750"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXZ37278.1"
                     /translation="MKKRDEKTPDYLMGFRRSLFRGAGFTGEALDRPLIGVVNSWGEI
                     SPATKHMQVLTKAAKDGIQSAGGTPVEFVLSGLCDGTCGGAPGANIYNMAWRDIAVAY
                     IETVAEANQFDALVFLPVCDEVVPAHLMAAARLNVPSILVCGGTMLPVRVGDREVWAG
                     DATAAFAELKCGKISEATYRRTEELCCSGAGACGVMGTGNTMQAFAEAIGMSLPGNGT
                     IAGVDPALEKMARQSGVQVMDLWRRNIRPRQIMSREAFENAVRVTLTLGGSTCAIFHT
                     IALAHEAEIPLDLSIFEEMSRNTPFIGNVQPSGPYPVKGLDDAGGIPAVMKELSPLLN
                     LGVSTVTGKTLRENIHGANVRNQDVIRPLVHPLHAEGGIAVLRGSLAPQSAIVKHVAV
                     LPQMKVHEGAAKVFDSEDAAGAAILAGGIVPGDVIVVRYAGPKGGPGMPCLYGSLWLL
                     KAKGLESSVALVTDGRLSGTIRGAAIGHVSPEAAEGGPIALLRDGDRIRIDLPGRKLD
                     VLLGQEELEERRRQWNPPQPRVKKGFMALYPRLVSSTHEGAYLKTQG"
     gene            4221..5417
                     /locus_tag="VEL68_14755"
     CDS             4221..5417
                     /locus_tag="VEL68_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HXZ37279.1"
                     /translation="MADLGIDFCGVKFKNPILAASAEPTLSAENMKRVIETGAGGLVA
                     KTVTDSEAMRRLTKMSKWRYLDEQHNVCRGKVPRLFTFYGRSGLAEEEPEEWMEELKE
                     AQAAALQNDCVLIGSVGGTIPENWAKLAKMVEDTGIKLIELNFGCPHPSEMKGAQTGM
                     LTGQDKNAASEITYAVAKAVKVPISIKLTPQVADVVEMVKAVKAAGASAVTIINRFVG
                     FALDIETGKPYINGWAGVGGPWVKPLTLRWVSKVYTGVGIPISGTNGAYDWKDAVEFM
                     MSGATNVQMCSVVMLKGYSYLKEVIKGLDDFLNRKGYKTAKEIVGIAAKSALTYAEME
                     GLPKERALINRELCTDCQRCLRSCFYNVIHVQDGQVTIGEECRGCGICTCVCPVPGAV
                     GLQAYG"
     gene            5414..6217
                     /locus_tag="VEL68_14760"
     CDS             5414..6217
                     /locus_tag="VEL68_14760"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ37280.1"
                     /translation="MSFPFNQESYGQLSMKGKGMFLQGKVAMVTGAGQGVGQGIANVL
                     AREGAKVVLAAIHVEKARKAAEIIESKGGEALAYGVNVLVMAQVKTMVETTLQTFKGL
                     HILVNNAVAPSVNVPFSQSKEEDWDQDLGVGLKGYLICTRAVINHFLSQNYGRIISIS
                     SSAGKVGSPNLAVYSAAKGAIIGFTKALARELAKTGITVNSVAPGAVNTPMQDRLSEE
                     FKAHMRSTIPMGRYANPEEIGETVAFLASDRANYVTGQVWSVDGGRTMQ"
     gene            6278..7474
                     /locus_tag="VEL68_14765"
     CDS             6278..7474
                     /locus_tag="VEL68_14765"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37281.1"
                     /translation="MKKKGILGLVVLAVFAGAGLWLHTDDAWAQAKTLKIGELAGLTG
                     GIAPYGSQLHNARLLAIEEINQKGGIKVGSVQYQLELVHLDAGPPAEAVKVFERLLTV
                     EKLNLILDGAYSSVEYALGPVLKAKKAIMIWSGGNDPATTVGIPNAFRNHFDGGAPFM
                     KANELFLKKMGAKKISTYGQSGHADFKRFVEEYLPKIPGFEVLSKDWFPFGEKDYFPV
                     LTKIKGLKPDAVITHGFFTDGVTMVKQAREVGLLPGILWLNQFAAAPGMMDEASRKVW
                     EGTYEILSSSLAPTTQAPAKSKNFFEAYFKKYGEKGGFGGWAEAGHDSVYILVKALEK
                     AGTVEDIPKIISAMQGLTTTEILELLQDYKPGKLFDSDGQAYPKIVCSEWKDGKQVPV
                     FADYGL"
     gene            7612..8481
                     /locus_tag="VEL68_14770"
     CDS             7612..8481
                     /locus_tag="VEL68_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37282.1"
                     /translation="MVQILVNGLVNGALLAVMAISLTIIFSILRVPHFGLGGIFVWGA
                     FVAYFAVTLWKLNFLWAMLLSAASMAALGVLVEKGAFKRLRASTEDAMFVSAIGVLIA
                     LENGALLLWGDKTRSIPVPQLDFIISIPGILRLPAMRVVIFIVAGLAIVGLHYFVKKT
                     RMGKAMRAVAQDRVAARLLGIPIDRVASYTFALGSAMAAVAGTLVGAAFTFSFDMGSL
                     NILKAFVVVVLGGLGSVGGAIIGGFFLGVVDSFTLTYITSEFKHLVSFSLLILVLVLR
                     PSGLFGQKLGRPQ"
     gene            8478..9440
                     /locus_tag="VEL68_14775"
     CDS             8478..9440
                     /locus_tag="VEL68_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37283.1"
                     /translation="MRRSSWKWVLLFLLGVLFPFIGQEGYLTHMAITILIFSILASSL
                     NLLMGYTGLVSIAHGAFFGIGAYTSGILMMRAGFPFLAALFMATAFTCLIALGLSIPS
                     FRTKGIYYVIVTIAFQLIVSEIYDGWYAMTGGGLGLRGIPKPGQISLPVLGTLSFTSK
                     FSYYYLAFIFALVIHLGIARLIKSPVGISLMAIRDNETKALMMGLNPLAYKVFVFVLA
                     SGLAGLAGSLYAHYLEFAHPDLFSFFVSVDLFLMVMLGGAGTFFGPPFGVFILEMIRE
                     VLHEFVALRLFLYGVMLVLIIIFLPEGLLKPLTSLAKRFVPGRT"
     gene            9508..10278
                     /locus_tag="VEL68_14780"
     CDS             9508..10278
                     /locus_tag="VEL68_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37284.1"
                     /translation="MALLQVQNLTKSFGGLTAVNKVSFSIEEKSIFGLIGPNGSGKTV
                     IFDCITGYQRPDQGRVFFRDREISGKRPNQIALQGVGRSFQLSGVFPKLTVYQNLIFS
                     VQEKTLGRNFLTTLRSGGGWLPKDLAKRIDQVLEFTGFQRVREEIVANLPYGQQKILE
                     FASLMVMNPDPLLYMLDEPFAGLTQGEIASYLSLMRERREKGRTFLLVEHNMRALMEI
                     CDFIVVLDHGEKIAEGCPEEIQANKGVVEAYLGHGVVA"
     gene            10262..10978
                     /locus_tag="VEL68_14785"
     CDS             10262..10978
                     /locus_tag="VEL68_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37285.1"
                     /translation="MASLLEINGLTSGYGRVSVLQDASLRVDRLEIVSVLGANGAGKT
                     TLLNTIAGVLKPSRGKILFDGEDVTGYKPEILAGRGLSLVRQGRSIFPFMTVQENLDM
                     GGYLCKAAPNFRSRLDTVFQLFPVLAERKNQWAGSMSGGEQKMLELGRALMLELKLLL
                     LDEPSLGLAPLIMNSIFGHIREFHKRGMTVLLVEQNVSLALQISHRGYLLELGRIKLE
                     GKKEELLKDPAVKKYYLGLS"
     gene            11042..12118
                     /gene="dctP"
                     /locus_tag="VEL68_14790"
     CDS             11042..12118
                     /gene="dctP"
                     /locus_tag="VEL68_14790"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ37286.1"
                     /translation="MKRVSWMSVTLVVCLSLFAISAKAQAQKPTKPVTLTFNWEGAYG
                     RANCLVWPFRPGGSFEQLVTKHTDGLVKLDIKEKLYGIMESAFAIGDGRIQAGTQSIP
                     AATGTYPLLDFGGIPGFFADPPQGAYQWAEAFVDPKMMAVWDKYSKPAGFKIIGAAIS
                     LANNTLYGNKAIKTLGDFSGVKARTSGRTQTSALKALGGSPITLSMAEVEDALYRGTV
                     DAITTSKNFGAERGLLDLCKFASVWPVTPVFSQFIAVNSKVWDRLDPFLQEGLMKAGA
                     QLTREMAPVVEQMDVSYTLWIRASKCQLILPEPPEVKKAMELMRPVVKEWLDTAGPYG
                     KEVLRIGGAYATGPSASFIAEMAK"
     gene            12256..12780
                     /locus_tag="VEL68_14795"
     CDS             12256..12780
                     /locus_tag="VEL68_14795"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ37287.1"
                     /translation="MDKILQRIERIAYVVGYGTGYILLGIIALTQIEVVTRYVLRQPL
                     ILCDELGGYSLLAITFLGLAYCAKEKGHIRITFLVERMSAKASGRVRVLTLILALIYI
                     GIASKVSWDFLILSVKRGMKSNSWLMTPLYWPQMVLPVGCTLFFLVLLVQLVRTIRDV
                     TAGVRVEEVSGEEY"
     gene            12820..14112
                     /locus_tag="VEL68_14800"
     CDS             12820..14112
                     /locus_tag="VEL68_14800"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ37288.1"
                     /translation="MSPEWLTVLLMAGLLILLGLGVEIAVGMGIIAAVSLLILDKPLI
                     QITWSAWESLNSFTLLAMPLFIFMGSVFSGSGAVQALFGALDKWIGFLPGGLASVVIA
                     ANAVFGAISGSSVAAAATFSLIAYPEMEKRGYAPKLSIGSIAVGGTLSVLVPPSLILI
                     VYGSWENQSVARLFAAAMIPGIILSLLLLVTVIIWVKINPVLAPRPPRFTWWDRILSI
                     KQLLPWLAVVITVMGSIFGGLMTPTEAAALGAVLSLVVAWGYGNLTYKIVKDSFLTAV
                     RVTAMIGFIIFAARLLSFGFQDMGVTERMSSYILGLGLGKYGIMVFLVVLYLILGMFF
                     DSMAMMLLTLPFVMPVLTNLGYDPIWWGVTFVVLAEIGLITPPFGLNLFTIHGVLPKF
                     SVVFIARSSLPLLGTMLLMVWILIFFPRLALWLPKVLY"
     gene            14166..14642
                     /locus_tag="VEL68_14805"
     CDS             14166..14642
                     /locus_tag="VEL68_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ37289.1"
                     /translation="MLKPLHLRVNGESAQLLVGEEETLLQVLRNRLMLTGTKQGCGEG
                     QCGHCTVILNGKAVNSCLTLALEAEGGEVMTIEGLAQEGRLHPIQKAYVEAGAIQCGY
                     CTPGMIMAIYSLLQENPQPSEDQIREGIKGVMCRCTGYYKVVEAVNRVAAEGQSHG"
     gene            14635..15597
                     /locus_tag="VEL68_14810"
     CDS             14635..15597
                     /locus_tag="VEL68_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXZ37290.1"
                     /translation="MDKPSFFRVATVAQAVRLKKENPQAFFMAGGTDLLILERHRLIQ
                     PKSILYLKGIPGLAEIRPEADGAVRIGACATLDAVVHHPLIRQNYPALAQASFAVASP
                     QIRNKATIGGNICLDSRCWFFNRSPFWRAEYPECRKAASGTKCYVLPHSRKGCFALQS
                     GDTVGSLVAMGAKVRLASDQGERVLGIEDFYLGDGIRYLDLKPGEMLAEILLPPPKGQ
                     GIFRKFRPQNNLDFATFNLTLFPPQEGTGSRIVVSSVASKPLRARKAEALFDRGAPGK
                     EVVDQAMKEISPISFVRGDVEFKKQVIAAYMTEAIAGSKAPLGE"
     gene            15608..17893
                     /locus_tag="VEL68_14815"
     CDS             15608..17893
                     /locus_tag="VEL68_14815"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXZ37291.1"
                     /translation="MESYRIITKRVTRPDALAKVTGQAKFTVDLNLPGMLIGKTLRSP
                     RHHARILHVDTAPALRVPGVKAVITSRDLPPVVYGFGDRRADMTVFATDRVRYKGDEI
                     AAVAAVDEEAATEALERMVVEYEELPAVFDPEEAVKDGAPQLHDDSPHNIGVRIKINK
                     GDMDRALAEAYLAREDRFDTQRVHQSYAEPYGCLVEWDANERITVYAGTMNSSGLQIM
                     LSRVLKVPIGRIRVIQTHTGGSFGSKVVLNSIYPASAVLSKKTGRPVKMVYSREEEYF
                     ASRPRFAGRYYVKTAVKKDGRILGRELNFFYDAGAYCDMAAAMIIVCSHRNDSVYRIP
                     AIRTDAKLVYTNKSPVGAYRGYGSPQNTFAFDSQLDMIAEDLGMDPAELRLKNATRPG
                     DVTVHGWKITSCGLPEAIEHVVAASGWKEKRGKRRRLNQGIGMACTIHEVDDRHSDGF
                     AGSNAHVEILQDGQAVIYSGEGEYGQGRDTTFCQIAAEVLGIPLESVRIILPDTDITP
                     YSLGPWGSRVTYSGGIAVFRAAEEAKRILLQEAGDLLEANPADLSIQEGKIFIKGLSQ
                     PKATVGEVAYFALFRKGGRRIRGSGQDEPATTKMDPTKQTSPCSTYSFACQAVEVEVN
                     DQTGEVKVLKLWTANDGGNIINPIGAEGQIEGQVLQGLGFAKTEEMVFRDGHLLTTDF
                     MTAGTPGPFDAPPLHIYFTKTYDPTGPFGAKGVAEVAAPPTAAAVANAVYDAIGVRVN
                     KLPLSPEKVLVAIKDQRKRSP"
BASE COUNT         3865 a         4942 c         5468 g         3905 t
ORIGIN      
        1 attttcgccg tggacctgaa attgcccggg atgcttcacg ccaaagtctt gcggagtccc
       61 atcccccatg ggcgaatttt gcgtatcgat acggaaaaag caaaaaatct accgggagtc
      121 gcggcgatcg tgaccgggaa ggatttttct tccctcttcg gagccaccat tggagatcaa
      181 ggtttcctgg ctcggggcaa agtgcgtctg gtgggggatg ccgtggcggc cgcggcggcg
      241 gttgatgcgg atacggcgga agaggccctt tctctgatca aagtagaata cgagccgctg
      301 cccgggcttt tcgatccagt cgacgctctg aagaatcggg aggttttaat tcacgaggac
      361 cttgggaaat acagcgtagc cccggggatc ttccccctgc cgggtacgaa catctgcaac
      421 cactttaggc tgcgcaaagg agatatggag cgggggttca aggaatctga ccttgtcctg
      481 gaagatacct acaggtccca cggagtgcag catgcccacc tggaacccca tgcagccatt
      541 gcccaggtgg atccttcagg ccgggtgcag gtctggacca atactcagac gccctatttc
      601 aaccgcaagg ccatcgccca ggccctgagc ctgcccttga acaaagtgcg cattacgggg
      661 acggccgtgg gaggcggttt tggcggcaag agctatctca aagccgaacc ggtctgcgtg
      721 gccttggccc tcaagacccg tgggcgtccg gtgaagctgg tttacacccg ggaggaggaa
      781 ttttccattg ctccggtgcg ccatcctacc attatccggt gcaaaaccgg catgaagagg
      841 gacggaacct ggatgggtca agagaccgag ctcatcttcg ataccggagc ttatgccgac
      901 atcggcccgc gagtcacccg caatgccggc ttttcagcca ccgggccgta taaagttccc
      961 catgtgcgca tcgactcgta ttgcgtctat accaacaact caatcggggg agctttccgc
     1021 ggatttggaa ccccccaggt cacctgggcc attgaatccc acctggattc catggctgaa
     1081 gggctgggga tagaccccct ggagctgcgc ttgaagaatg ccgtggaaga gggctcgatt
     1141 tccgccacgg gccaggctct ctacagtgtg ggcttaaagg ataccctgcg tcaagcagca
     1201 gataagatcg gctgggggca gggccggacg ccttttcggg gcaaagggat cgcctgcatg
     1261 cataaatcca cggttacccc ctcggcatcc tccgccttcg tgaagttgaa cgaagataag
     1321 tccgtaaccc tgctggtaag tacagtagaa atgggccagg gagcttccac gctcctggcc
     1381 cagatagtgg ctgaagaatt gagtgtcccg gtggagaatg tctccgtcgt ctccccggac
     1441 acggacgtca ctccctacga tatggcttcg gtttccagtc gttccacttt tttcgcaggg
     1501 aatgcggtgc gccgcgcggc catcgatgcc cgggagcagc ttctggaaat tgcctctgaa
     1561 atactcgaag ccagccccaa ggacctggtc ctggagagag gtaggatctt cgtgcggggc
     1621 gtgcccgata gagccattcc ccttgcagac ctccctctcg gggaggcttt ctacgtgggg
     1681 gccttgggac gggggaaggg cagaccggtc ctcggccgcg gctcttatgc cgtggaggat
     1741 gctaccgccc ttgaccggga gacgggccag ggcaagaacc caagcgcctt ctggatgtac
     1801 gcgacccaag ctgccgaggt ggaagtcaac ccccggaccg gccaggttaa ggttctgcgc
     1861 ctgacctcag cccatgacgt cggccgggcg ctccatccgg tttccatcga aggacaaatt
     1921 cagggagccc tggtcatggg catcgggact accctcttcg aagacctgga tatgcgcgag
     1981 ggcaaggcgc agaaccataa tttttcctct tacaagcttc cttccgctct ggatattcct
     2041 gaaatgatac ccatcattgt tgaggcgccg gatcggaatg ggccttatgg ggccaaggga
     2101 ttaggggaac cagccctttc ccctactgcc gcatccattg ccaacgccat ccatgccgcg
     2161 accggggtgc gggtcaaaga cctgcccatt acgcccgaaa agattttagc gggactggtc
     2221 caaaaggaga atccatgaag aaaagagatg aaaaaactcc cgattacctg atgggtttcc
     2281 gccggtccct atttcgaggg gccggcttta cgggagaggc tctggaccgc ccactcattg
     2341 gagtagtcaa cagctggggc gaaatcagcc cagctaccaa acacatgcag gttttaacga
     2401 aagcggccaa agacggaatt cagagcgcag ggggaacgcc ggtggagttt gttctctcgg
     2461 gcctctgcga cggaacctgc gggggagccc cgggggcgaa catctataat atggcctgga
     2521 gggacatcgc cgtcgcctac atcgagaccg tagccgaagc aaatcaattt gatgctctgg
     2581 tcttccttcc agtctgcgac gaggtggttc cggcccacct catggcggcc gcccggctga
     2641 atgtaccctc cattttggtt tgcggcggca ccatgcttcc ggtcagggtg ggggacagag
     2701 aggtctgggc gggggatgcc acggcggcct tcgctgagct gaagtgcggg aagatttctg
     2761 aagccacgta tagacggacg gaagaacttt gctgctcagg ggccggagcg tgcggcgtaa
     2821 tggggacggg caacaccatg caggccttcg ccgaggctat cggcatgtct cttcctggca
     2881 atggaaccat tgccggggtg gacccggcgc tggaaaagat ggcccggcaa tcaggagtcc
     2941 aggtcatgga cctttggcgg cgaaatatcc gtccccggca gatcatgagc cgggaggctt
     3001 tcgagaatgc cgtccgggtc actctcactc tgggaggttc cacctgtgcc atcttccata
     3061 ccattgccct ggctcacgaa gcggaaattc ccctcgacct ttccatcttt gaagagatga
     3121 gccggaatac cccctttatc ggaaatgtcc aacccagcgg accttatccg gtgaaagggc
     3181 tggatgatgc gggggggatc ccggcggtga tgaaggagct ttcccctttg ttaaaccttg
     3241 gcgtatcgac ggttaccgga aagaccctgc gggaaaatat tcacggagca aatgttcgca
     3301 accaggatgt cattcgtcct ctggtccatc ctttacatgc cgaaggaggc attgcggtgc
     3361 tgcgagggtc tctcgccccg cagagcgcca tcgtcaagca tgttgccgtc ctcccccaga
     3421 tgaaggtgca cgaaggagcg gccaaggttt ttgactcgga ggatgcggcc ggggcagcga
     3481 ttctggcagg tggaatcgtt cccggagatg ttatcgtggt tcgctatgcc gggcccaagg
     3541 gtggccccgg catgccttgc ctatacggtt ccctctggct tctcaaagcc aagggcctgg
     3601 aaagcagtgt ggccctcgtg accgatggcc gtctttccgg caccatccgg ggcgcggcga
     3661 ttggccacgt ctctccggag gctgccgaag gagggccgat cgcccttttg cgggatggag
     3721 accggattcg gattgatctt cccgggcgga agctggacgt gctcttaggc caagaggaat
     3781 tggaagagag gcggcgacag tggaatcccc cgcagccccg ggtgaagaaa ggattcatgg
     3841 ccctctatcc ccgattggtg tcttcgaccc atgaaggagc ttatttaaag actcaggggt
     3901 gagaagttgg attgagaagg gagaaaagaa tagcgggtaa agataacaag gataaaaaat
     3961 gctagaatgg gtgcttctgt aaaaagtcat ggttattccg ctttcgtcat ttccgcggaa
     4021 gcgggaatcc agtaaaatga tgtagttctg ggctcccgct tgcgcgggag cggcggcctt
     4081 gcttgttttt tacgaaatca tcaaaattga aaatgcccaa aatcatgaac gaaaaagatt
     4141 tcataatttc caacgcaaaa ataaacgagc gccgagtaac caccaacggg tgacgagcat
     4201 cgagcaaagg ggaggtaagc atggccgatt taggaataga tttttgcggt gtgaagttca
     4261 agaacccgat cctggcggca tcggcggagc cgacgctctc ggcagagaac atgaagcggg
     4321 tcatcgagac cggagccggc gggctagtag ccaagacggt taccgattct gaagccatgc
     4381 gacggctgac caagatgtcc aaatggcggt acctggatga gcaacataat gtatgccggg
     4441 gaaaggttcc ccgactcttc actttctacg ggcgcagcgg tctcgcggag gaagaaccgg
     4501 aagaatggat ggaggagctc aaagaggctc aggctgcggc gctgcagaac gactgcgtgc
     4561 tcatcgggag cgtgggcgga accattcctg agaactgggc gaagctagcc aagatggtgg
     4621 aggacaccgg gatcaagctc atcgagctga atttcgggtg ccctcacccc tcggagatga
     4681 aaggggccca gacggggatg ttgaccggcc aggacaagaa tgccgcttca gaaataacct
     4741 acgcggtggc caaagcggtg aaggtcccca tcagcatcaa actcactccg caggtagcgg
     4801 atgttgtaga gatggtcaag gcggtgaaag ccgccggcgc ctcggcggta accatcatca
     4861 accgcttcgt gggtttcgcc ctggatatcg agacgggcaa accctacatc aacggttggg
     4921 ccggggttgg gggtccctgg gtgaagcctt tgaccctgcg ctgggtctcc aaagtttaca
     4981 ccggggtggg cattcccatc tccgggacca acggggccta tgactggaag gacgcggttg
     5041 agttcatgat gtctggagcg accaatgttc agatgtgttc ggtagtcatg ctcaagggat
     5101 acagttacct taaagaagtg attaaagggc tggatgattt tttgaatcgc aaaggataca
     5161 agacggccaa ggagatcgtg ggaatcgccg ccaagtcggc cctgacctat gcggagatgg
     5221 agggcctccc caaagaacga gcgctaatca atcgggaact ctgcaccgac tgccagcgat
     5281 gcctgcggtc ctgcttctac aacgtgatcc acgtgcaaga tgggcaggtg accatcgggg
     5341 aagagtgccg gggttgcgga atctgcacct gcgtttgtcc ggtgcccggc gcagttggct
     5401 tgcaggctta tggatgagct ttcctttcaa ccaggagagc tatggacaat tatccatgaa
     5461 agggaaaggc atgtttctcc aaggcaaagt tgcaatggtc accggtgcag ggcagggagt
     5521 gggtcagggg attgctaatg ttttggcccg ggagggggcc aaggtagtcc tcgcagcaat
     5581 acatgtagaa aaggcccgga aggcggctga aatcatagaa tccaagggag gagaagccct
     5641 ggcctatggc gtgaatgtgc tggtgatggc ccaggtcaag accatggtgg agacgacgct
     5701 ccagaccttt aaggggttgc acatattggt aaacaacgcc gtggccccaa gcgtcaacgt
     5761 tcctttttct caatccaaag aagaggactg ggatcaggac ctgggggttg gtctgaaggg
     5821 atacctcatc tgcacccggg cggtgatcaa tcatttcctg agtcaaaatt atgggaggat
     5881 catcagtatc tcgtcctccg ccggcaaagt ggggagtcca aacctggccg tttattctgc
     5941 agccaagggg gccatcattg gctttacgaa ggcccttgcg cgcgaactgg ccaagaccgg
     6001 aatcacggtg aatagtgtag cccctggagc ggtgaacacc cccatgcaag accgtctctc
     6061 agaagaattc aaggcccata tgcgttccac catacccatg gggcgatatg ccaaccccga
     6121 agaaatcggg gaaaccgtgg ccttcctggc ttcggaccgg gcgaattatg ttaccggtca
     6181 ggtctggagc gtagatggcg ggaggaccat gcagtgattt ttgggacaca ggagaacgga
     6241 gatttccagg atattaatta gataaggagg gggaaccatg aagaagaaag gaatcctggg
     6301 tttggttgtt ctggcggtgt ttgcgggtgc cggtctttgg ctgcacaccg atgatgcatg
     6361 ggcccaagcc aagaccctaa agattggaga gctggcaggc ctaaccggag ggattgctcc
     6421 ctatggatcc caactgcaca acgcccgact cctggcgatc gaggagatca accagaaggg
     6481 cgggatcaaa gttgggagtg tgcagtacca actcgaactg gtccacctgg atgccgggcc
     6541 gcccgcagaa gcggtgaaag tattcgagag gttgctcacc gtggagaaac tgaacctgat
     6601 cctggacggc gcttattcct ccgtggaata tgccctggga cccgtcctca aggctaaaaa
     6661 ggccatcatg atctggtcgg ggggtaatga cccagccacc accgtaggga ttcccaatgc
     6721 cttccggaac catttcgacg ggggcgcccc tttcatgaag gccaatgaac ttttcttgaa
     6781 gaaaatgggg gccaaaaaaa tctccaccta tggccagagc gggcatgccg acttcaagcg
     6841 ctttgtggag gaatatctgc cgaagattcc cgggttcgaa gtcctgtcca aagactggtt
     6901 tccttttggc gaaaaagact atttcccggt cctgaccaaa atcaagggtt tgaaaccgga
     6961 tgccgtgatt acccatggtt tcttcaccga tggggtgacc atggttaagc aggcccggga
     7021 ggttggcctt ctcccgggaa ttctctggct caatcagttc gccgctgctc cgggaatgat
     7081 ggacgaggcc tcccgaaaag tctgggaggg gacttacgaa atcctcagct ccagcctggc
     7141 ccccactacc caagccccgg ccaaatcgaa gaatttcttc gaggcctatt tcaaaaaata
     7201 tggcgaaaag ggaggctttg gcggatgggc ggaggctggc cacgacagcg tctatattct
     7261 ggtcaaggca ttggagaagg ccggcacagt cgaggacatt cccaagatca tcagtgccat
     7321 gcagggcctg accaccacgg agatcctcga gctcctgcag gattacaaac ccggaaagct
     7381 ctttgactcg gacgggcagg cctaccccaa gatcgtttgc tccgagtgga aggatgggaa
     7441 gcaggttccg gttttcgcgg actatggtct ttaaaaaatc ctctctaacc ccctttataa
     7501 aaggggagga ttgggagatt ttataggggc ggccccgtgg ccgccctcaa tcgggcaacc
     7561 aaagagagtt gccccgcatt tttttaggca tcaggagatt tcaacgaacg catggtccag
     7621 attctggtga acgggctggt taacggcgcc ctcctggcgg ttatggccat cagcctgacc
     7681 attatcttca gcatcctgcg ggttccccat tttggcctgg ggggaatctt cgtctgggga
     7741 gccttcgtgg cctatttcgc ggttaccctc tggaaattaa acttcctgtg ggccatgctc
     7801 ctctctgcgg cgagcatggc ggctttggga gttctcgtcg agaagggcgc cttcaagcgg
     7861 ctgcgggcct ccacggaaga cgccatgttc gtctccgcca tcggggtgct catcgccctg
     7921 gagaatggtg ctcttcttct atggggtgac aagacccggt ctattcccgt tccgcagctc
     7981 gacttcatca tttccatccc ggggatattg cgtctgccgg ccatgcgcgt cgtgatcttt
     8041 atcgtcgccg gcctggctat tgtggggctg cattacttcg tgaagaagac tcgcatgggg
     8101 aaggccatga gggcggtagc ccaggatcgg gttgccgccc ggctcctggg aatccccatc
     8161 gaccgggtgg cctcctatac gtttgccctg ggtagcgcca tggccgcggt ggccggaacc
     8221 ctcgtcggag cggcttttac cttttccttc gacatgggca gcctgaatat cctcaaagcc
     8281 ttcgtggtgg tggttttggg aggattgggc agcgtggggg gggccatcat cgggggcttc
     8341 tttttgggag tggtggacag ctttaccctc acttacataa catcagagtt caagcatttg
     8401 gtgtccttca gcctgctgat cctggtgctg gtgctccggc cttcgggcct cttcgggcag
     8461 aaactgggga ggccccaatg agaagaagtt cctggaaatg ggtccttctc ttcctcctgg
     8521 gagtcctttt tccctttatc ggccaagaag gatatctcac ccatatggcc atcactatcc
     8581 tgatcttctc tattctggcc tccagcttga acctcctcat gggctatacc ggcctcgtct
     8641 ccatcgccca tggagccttt ttcggcattg gcgcctatac ttcgggcatc ctaatgatgc
     8701 gtgccggttt ccccttcttg gctgccctct ttatggctac tgcttttacc tgcttgatcg
     8761 ccttgggact cagtattcct tctttccgga ccaaggggat ttattacgta atcgtgacta
     8821 tcgccttcca gctcattgtc agcgaaatct acgatggctg gtatgccatg accggcgggg
     8881 ggctgggttt gagagggatc ccgaagccgg gtcaaatctc tttgccggtc ctcgggactt
     8941 tgagctttac gtccaagttt agttactact acctggcctt catctttgcc ttggtcatcc
     9001 atctcggaat tgcccggctc ataaagtcac cggtgggcat ttccttgatg gccattcgcg
     9061 ataatgagac caaagccttg atgatggggc tgaaccctct ggcttataag gtctttgttt
     9121 ttgttctggc ctccggcctg gcgggtttgg ctgggtccct ttacgctcat tacctggagt
     9181 tcgctcatcc ggacctgttc agcttttttg tctccgtgga cttgttcctg atggtcatgc
     9241 tcggaggggc cgggactttt ttcggccctc cttttggggt ttttatcctg gagatgatcc
     9301 gggaagttct ccacgagttc gttgccctgc ggcttttcct atacggcgtg atgcttgttc
     9361 tcattatcat ctttttaccc gaaggactct tgaaacctct cacctccctg gcgaaaagat
     9421 tcgttcccgg gagaacgtag ggacttaaag ggactcggat ttcccgtttc gggtttatgg
     9481 atataaagtt catgcatggg gggtgacttg gccctacttc aggtccagaa tctgaccaaa
     9541 tcctttgggg gcctcacggc ggtcaacaag gtctcattct cgatcgaaga gaaatccatc
     9601 ttcggcctga tcggcccgaa cggatcgggc aagacggtca tctttgactg tatcaccgga
     9661 tatcagaggc ctgatcaggg acgggtcttt tttcgggatc gggagatcag cgggaaaagg
     9721 ccgaaccaga tcgccctgca gggcgtggga aggtcctttc aattgagcgg agtttttccc
     9781 aagctgaccg tttaccagaa tttgatcttt tcggtccaag agaaaaccct cgggcgcaat
     9841 tttctcacca ccctgcggtc gggaggaggt tggctaccga aagacttggc caaacgcatc
     9901 gatcaggtcc tcgagtttac cgggttccaa agggtaaggg aggagattgt cgccaacctg
     9961 ccttacgggc agcagaagat tctggaattc gccagcctca tggtgatgaa tccggacccc
    10021 cttctctaca tgctcgacga acctttcgcc gggctgaccc agggcgaaat cgccagctac
    10081 ctttccttga tgagagagcg gcgggaaaaa ggacggactt tcctccttgt ggaacacaat
    10141 atgagggcct tgatggaaat ctgtgacttc atcgtagtat tggatcatgg cgagaagatc
    10201 gccgagggtt gcccggaaga gatccaggcg aacaaagggg tggtggaggc ctatctcggc
    10261 catggcgtcg ttgcttgaaa ttaacgggct gacgagtggt tacggcaggg tttccgtcct
    10321 ccaggacgcc tccctgaggg tggatcgatt ggaaatcgtg agcgtcctgg gggccaatgg
    10381 agcggggaag accaccctgc tcaataccat cgcgggcgtt ctcaaaccat cccgaggaaa
    10441 gatcctcttt gacggggaag acgtgactgg ctataagccg gaaatcctgg cgggaagggg
    10501 cctttccctc gtgcggcagg ggaggtccat tttccccttt atgaccgttc aggaaaactt
    10561 ggatatggga gggtatctct gcaaggccgc cccaaatttt cgaagccgcc tggatacggt
    10621 cttccagctc ttccccgtgt tggccgaaag aaaaaaccaa tgggccgggt ccatgagcgg
    10681 aggggaacaa aagatgttgg agctgggcag agccctgatg ctggagctca agctgctcct
    10741 cctcgacgag ccttccttgg gtctcgctcc cttaatcatg aattccattt ttggacatat
    10801 tcgcgaattc cataagaggg ggatgaccgt tctcctggta gaacagaacg tatccctcgc
    10861 cctgcagatt tcccatcggg gatatttgct ggagctggga cgaattaaat tggaagggaa
    10921 aaaggaagaa cttttgaaag accccgcagt aaagaaatat tacctggggc tcagctaaga
    10981 atgaccgccg gctagcttcc ttgcggtggg aaaaccattg attcagaaag gagagaagaa
    11041 gatgaaaaga gtaagctgga tgtcagttac ccttgtcgtc tgtttatccc ttttcgcaat
    11101 ctcggccaaa gcgcaggccc aaaagccgac gaaaccggtc acccttactt tcaactggga
    11161 gggggcctac gggcgggcga actgcctggt ttggcccttt agacccggcg gaagcttcga
    11221 gcagctggtc accaaacata ccgatggact ggtcaagctg gacatcaaag agaaacttta
    11281 cggcatcatg gaatctgctt ttgccatcgg ggatggccga atccaggccg gaacccaatc
    11341 catccccgcg gccaccggaa cctatccgct cctggatttt ggcggaattc caggattctt
    11401 cgccgacccc ccccaaggag cctatcaatg ggccgaagcc tttgtggacc ccaagatgat
    11461 ggccgtatgg gacaaatatt ccaaaccggc ggggttcaag atcatcgggg cggccatcag
    11521 cctggccaat aacaccttgt acggaaacaa ggccatcaag acccttgggg atttcagtgg
    11581 agtaaaggcc agaacctccg ggcggaccca gacctcggcc ctcaaggctc ttggagggag
    11641 cccgatcacc ctgtccatgg ccgaggtgga agacgccctt taccgcggca ccgtcgatgc
    11701 catcaccacg agcaagaact ttggggccga gcgggggctt ctcgacctgt gcaagttcgc
    11761 ctcggtttgg ccggtaaccc cggtcttttc ccaattcatc gccgtcaatt ccaaggtgtg
    11821 ggaccggctg gaccccttcc tgcaggaggg gctgatgaaa gccggcgccc agttgacacg
    11881 ggagatggcc ccggtggtgg aacagatgga cgtctcctac acgctctgga tccgggcgag
    11941 caaatgccag ctcatcctgc ccgaaccacc ggaggtgaaa aaggccatgg agcttatgcg
    12001 cccggtggtg aaagaatggc tggatacggc agggccctat ggcaaggaag ttctgcgcat
    12061 cggaggagcg tatgcgaccg gcccctcggc cagttttatc gccgagatgg ccaaatgaga
    12121 aaaaggagaa agcttcccgc ccggagtcga accggccagg accgagtcta aagaaggaaa
    12181 tccttcggct ccgggcgaca gtcttcccga ccttgggaaa ccgcggtttc ggtgagtttc
    12241 gggagaatga ttcatatgga taagatcctg cagagaatcg aaagaatcgc ttacgtcgta
    12301 gggtacggga ccgggtatat cctcctgggg atcatcgccc tgacccagat cgaagtggtt
    12361 actcggtatg tcctgcgcca gccattgatc ctttgcgacg agttgggggg ctacagcctg
    12421 ctcgccatca cttttctcgg cctggcctac tgcgccaaag agaaggggca tatccgcata
    12481 actttccttg tggagagaat gtcggcgaaa gcatccggcc gagtccgggt cctcaccctg
    12541 attctggcct tgatctatat cggcattgcc agcaaggtga gctgggattt cctgatcctc
    12601 tcggtgaaac gcggcatgaa gtccaactcc tggctcatga cccctctgta ttggccgcag
    12661 atggtcctcc cggtcgggtg cacgcttttc tttctggtcc tcctcgtcca gctggtgagg
    12721 acgatccggg acgtgacagc gggggtgagg gtggaagagg tgtcggggga agaatattga
    12781 gaagcggcgg cgaaggcccc ctgcaaagga gacagcggaa tgagtcctga gtggttgacc
    12841 gtgcttctga tggccggcct gttgattctt ctgggcctgg gagtggagat cgcggtggga
    12901 atgggaatca tcgccgccgt gagtttgctg atcctcgaca agcctctgat ccagattacc
    12961 tggtcggcgt gggaatcctt gaattcgttt accctcctgg ccatgcccct cttcatcttc
    13021 atggggtcgg ttttttctgg cagcggagcg gtccaggccc tcttcggggc gctggataaa
    13081 tggatcggct tcctacctgg aggcctggct tccgtagtga tcgcggccaa cgcggtcttc
    13141 ggagccatat cgggctccag cgtggccgcc gctgcgacct tcagcctaat tgcctatccg
    13201 gagatggaaa aacgggggta cgccccgaaa ctttccatcg gctctatcgc cgtggggggc
    13261 accctgagcg tcctcgtccc tcccagcctg atcctgatcg tctacggctc ctgggagaac
    13321 cagtcggtgg cccgcctctt tgccgcggcc atgattcccg ggatcatcct ttccctgctc
    13381 ctgctggtca ctgtaatcat ctgggtcaag atcaaccccg tcctggcccc gcgacctccc
    13441 cgattcacct ggtgggacag gattctctcc atcaagcaac tgctcccctg gctggccgtg
    13501 gttattacgg tgatgggttc gatcttcggc gggctgatga cccccaccga agcggccgct
    13561 ttgggggccg ttctctccct ggtggtggcc tgggggtatg gaaacctgac ttacaagatc
    13621 gtcaaggaca gcttcctcac agcggtcagg gtcacggcca tgatcgggtt catcatcttt
    13681 gccgcgcggt tgctttcctt cggattccag gatatggggg ttacggaaag gatgtcttcc
    13741 tacatcctgg gcctgggtct tgggaagtac gggatcatgg tgttcctggt ggttctttac
    13801 ctgattctgg gcatgttctt cgattccatg gccatgatgc tcctgacgct cccctttgtc
    13861 atgccggtgc tgacgaacct ggggtacgat cccatatggt ggggggtgac cttcgtggtg
    13921 cttgctgaga tcgggttaat caccccgcct ttcggcctga acctcttcac gatccacggg
    13981 gtcctcccca agttttccgt cgtattcatc gccaggagtt ccctgcccct tctgggcacg
    14041 atgctcttga tggtctggat cctcattttt ttcccccggt tggccctctg gctgcccaag
    14101 gtgctgtatt aaagcgaacc cgcgaagggc ctcattcacg gaaggggggc gcgaaaggaa
    14161 aagaaatgct caagccgtta catctgcgcg taaacgggga atcggcccag cttctcgtag
    14221 gggaggagga aacccttctc caagtcttac ggaaccggct catgctgacg gggacgaagc
    14281 agggatgcgg ggagggccag tgcgggcact gcacggtcat cctgaatgga aaggcggtga
    14341 acagctgtct gaccctcgcc ctggaagccg aggggggcga agtcatgacg attgagggtt
    14401 tggcgcaaga gggccgtctt caccccatcc agaaggccta cgtggaagca ggagccattc
    14461 aatgcggtta ctgcacccct ggtatgatta tggccattta ttccctgctg caggaaaacc
    14521 ctcagcccag cgaggatcag atccgggaag gaatcaaggg cgttatgtgt cgctgcaccg
    14581 gctattacaa ggtggtcgag gcggtcaacc gcgtggctgc cgaagggcag tcccatggat
    14641 aaaccctcct tcttccgggt ggcgactgtc gctcaggcgg tccgcctgaa gaaagaaaac
    14701 ccccaggcct tcttcatggc cggagggacc gatctattga ttctcgagcg gcacaggctg
    14761 atccagccca aatccatcct gtatttgaag ggcatcccgg ggctggccga aatccgcccg
    14821 gaggctgatg gcgcggtgag gattggcgca tgcgctaccc tggacgccgt cgtccatcac
    14881 ccgttaatcc ggcaaaatta tcctgccctg gcacaggcct cgttcgcggt agcctcccca
    14941 caaattcgca acaaggccac catcgggggg aatatctgcc tggacagccg gtgctggttt
    15001 ttcaaccgct cgcccttctg gcgtgcggag taccccgagt gccgcaaagc ggccagcggc
    15061 acgaagtgtt atgtcctacc ccacagccgg aaaggctgct ttgccctgca atcgggggat
    15121 acggtgggct cgctggtggc aatgggggcc aaggtgcgcc tggcctcgga tcaaggggaa
    15181 agggttctgg ggatagagga tttctacctg ggtgacggca tccgctattt ggacctgaag
    15241 ccgggcgaga tgctggccga gatccttctg cccccgccga agggccaggg gatcttcagg
    15301 aagttccggc cgcagaataa tctggatttc gccactttca acctcaccct ttttccgccg
    15361 caagagggga ccggttcccg aatcgtggtg tcttccgtgg ccagcaaacc cctccgggcc
    15421 cgcaaggcgg aggccctgtt tgaccggggt gctcccggga aagaagtggt ggaccaggcg
    15481 atgaaggaaa tatccccgat ttcctttgtc cggggggacg tggaattcaa aaagcaggtc
    15541 attgccgcct atatgaccga agccattgcg ggatccaagg ccccgttggg agaataggga
    15601 aaagccgatg gaatcctatc gaattattac caaaagggta acccggccgg atgccttggc
    15661 caaagtcacc gggcaggcga aatttacagt ggacctcaac cttccgggca tgctgatcgg
    15721 aaagaccctg cgcagccccc gccatcatgc ccgcattctg catgtggaca ccgctccggc
    15781 tttgagggtc cccggggtca aggcggtgat cacctcccgg gaccttccgc cggtggttta
    15841 cggcttcggc gaccgtcggg cggatatgac cgtcttcgcc acagaccgcg ttcggtacaa
    15901 gggcgatgag attgctgccg tggccgctgt ggacgaagaa gccgccaccg aggcgctgga
    15961 gcgcatggtg gtggagtacg aagaattacc cgctgtcttc gatcccgagg aggccgtcaa
    16021 agacggtgcc ccccaacttc acgatgattc gccccataac atcggggttc ggatcaaaat
    16081 taacaaggga gacatggacc gggctcttgc tgaagcctat ctagcgcgag aagaccggtt
    16141 cgatacccag agggtccatc agtcctatgc cgaaccctat ggctgtttag tggagtggga
    16201 cgccaacgaa aggatcaccg tttacgcggg gacgatgaat tcttcggggc tccagatcat
    16261 gctttcacgc gtgctcaaag tgccgatcgg ccggattcgc gtcattcaaa cccacaccgg
    16321 cgggtccttt ggaagtaaag tggttctcaa ttcgatctat cctgcctcgg cggttttgag
    16381 caaaaagacc ggccgcccgg tcaagatggt ttattcccgg gaagaagaat atttcgccag
    16441 ccggccccga tttgcgggcc gatattatgt gaagaccgcg gtgaagaaag acggaagaat
    16501 cctggggcgg gagctgaatt ttttctatga cgccggagcc tattgcgaca tggctgcggc
    16561 catgattatt gtctgttctc atcggaacga tagcgtctac cggattcccg ccatccgtac
    16621 cgatgccaag ctggtctata ccaacaagag cccggtaggg gcctatcggg gatatggaag
    16681 cccccaaaac acttttgcct tcgattctca acttgacatg atcgccgaag acctggggat
    16741 ggaccctgcc gaattgcggt tgaaaaacgc cactcgtccc ggcgacgtta cggtccatgg
    16801 ctggaagatc accagttgcg gacttcccga ggccatcgag cacgtggtag cggcctccgg
    16861 ttggaaagag aaaaggggca agaggcgccg cctcaatcag ggaatcggga tggcctgcac
    16921 gatccatgag gtggacgacc gccattccga cggcttcgcc gggagcaatg cccatgtaga
    16981 aatcctgcag gacggacagg cggtcattta ctccggcgag ggggagtacg gtcaggggcg
    17041 ggacacgact ttttgccaga tcgccgccga ggtcctggga attcctctag agtccgtccg
    17101 gatcattctc cccgatacgg atatcacccc ttattccttg ggcccttggg gttcacgggt
    17161 cacttactca ggcggcatcg ctgtgttcag agcggcggaa gaggccaagc gcattcttct
    17221 tcaagaagcc ggggacctgc tcgaagccaa tccggctgac ctgagcattc aggagggaaa
    17281 gattttcatt aagggcctat cccagcccaa ggccacggtg ggggaggtgg cctactttgc
    17341 cctcttccgc aagggaggaa gacgaatccg tggcagcggc caggacgagc cagccacgac
    17401 caagatggac ccgacgaagc agaccagtcc ctgctccact tactcttttg cctgccaggc
    17461 cgtggaggtt gaggtcaatg atcagaccgg agaggtcaag gtcctgaaat tgtggacggc
    17521 caatgacggc ggcaatatca tcaatcccat cggggcagaa ggacagattg aaggccaggt
    17581 gctccagggg ctggggttcg ccaagacgga agagatggtc ttccgggacg gccatctttt
    17641 gaccaccgac tttatgactg cgggaacccc tgggcccttt gacgccccgc ccctccatat
    17701 ttattttacc aaaacctacg acccgacggg tccattcggg gccaagggag ttgccgaagt
    17761 cgctgcgcct cccacggctg cggcggtggc caacgccgtc tatgacgcca tcggagtgcg
    17821 cgtcaataag cttcctttat ccccagaaaa agttcttgtg gccatcaagg atcagagaaa
    17881 gcgcagtccg tgaaaaatgt cccccccatt gtcctccgtc gggaaagtga gctttgccgc
    17941 gagctcggct gggcgagaag gggaataggt agggtgagga atcgaggcgg ttcgccagcc
    18001 gtcccgaccg gtcggaaaag gtcgaaaaat ttgccaggaa cacttggcaa agaagaaatt
    18061 gcagcttaag ggggttcgca tggagtttga atatttagag gaagtaaaga agctgctgca
    18121 tggaaagagt taccaagtcg tgggaacgga tgtgaagagg gtggattccc tcgaaaaagt
//
LOCUS       DASPRR010000404         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2188712, whole genome shotgun sequence.
ACCESSION   DASPRR010000404 DASPRR010000000
VERSION     DASPRR010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188712"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VEL68_14820"
     CDS             complement(<1..365)
                     /locus_tag="VEL68_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37292.1"
                     /translation="MKEKAKAIIQSPILYIALIFLILPYVFPVIGGYTDLGTEIMIMA
                     LFALAYNMLLGHTGLMSFGHGAYFGIGAYALGLTQLHFSSNIWVCLLFAMLAAGLAAF
                     LIGLVIIRKSGIYFGLLTVA"
     gene            complement(377..1246)
                     /locus_tag="VEL68_14825"
     CDS             complement(377..1246)
                     /locus_tag="VEL68_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37293.1"
                     /translation="MEYILSQLFNGIILGLLYALIALGLTLILGVMEVINFVHAALFT
                     FGAYLSLVLTGYIGFWPSLIIAPFIVGLIGILYETLTFRRVYGKDPLFGLLLAFGLVL
                     ASTEVIRMIWGPTGYALNPPRELVGSINLGFMLYSKYRIFAGLFTLVLIFLMWAFLEK
                     TTYGMVIKAGVSDSEMIGALGKNLPKVRTLVYSIGSIFAGIAGVLLAPLQGVKPDMGM
                     AIIMPAFVVIVVGGVGSFLGTIIGSLLVGLTVTIVVMVAPRISDLAMYVILAIIILKR
                     PRGLMGEKSGLEA"
     gene            complement(1278..1463)
                     /locus_tag="VEL68_14830"
     CDS             complement(1278..1463)
                     /locus_tag="VEL68_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37294.1"
                     /translation="MPGQVFAGLSTWGLIMALRLLLGKAMVGMVSKAAVGDAAMREAL
                     GKNLKKVRKPIASIQKF"
     gene            complement(1510..2667)
                     /locus_tag="VEL68_14835"
     CDS             complement(1510..2667)
                     /locus_tag="VEL68_14835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ37295.1"
                     /translation="MAGAPFLYFCTEWIWIGRGSLEQAIPEAKKLGAQKALIITDPGV
                     HQAGISARVQGALEKAGIKSEIFSSIKPEPPLELVDQCAEIARKGKMDLIVGVGGGSS
                     MDTAKGASVVVTNGGSVSKYFGIDQVLKPGVPTFLIPTTAGTGAEATRNAIFKDLKAK
                     AKKAIVSRHILPRVAIVDAELMRTTPPGVTAHTGIDALGHALESFMSKNANPLSEMYS
                     LQTIKLVAGNLQRAVENGNDMDARENMALAALCGGVSLMAGAGAIAATSYPVEGHFDV
                     PHGLGNALMMPAVVRYNTPGNIPKAKRICEQMGIDIRGLSDQEIADRLVEKISDLFGK
                     IGIPLGLGKRGVKEKDLPMLAAEAFQNKRLVDLNPRELTVESIVEIYRQAL"
     gene            complement(2763..>3165)
                     /locus_tag="VEL68_14840"
     CDS             complement(2763..>3165)
                     /locus_tag="VEL68_14840"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ37296.1"
                     /translation="NAKYNGIRQFDDCLGTCRIASPHPKLVLECFNAVTGWNWTLEDA
                     FTVGRRIVNLLRVFNLKHGMRMEDEKPSVRYGSTPTDGPTKGKNILEKWPWMVQNYFR
                     LMGWDERGKPLSETLEKLGLTELIEDLRSLR"
BASE COUNT          723 a          916 c          830 g          696 t
ORIGIN      
        1 gcgaccgtca gaaggccgaa atagatcccg ctcttccgga tgatcaccag gccgatcaga
       61 aacgccgcca gtcctgcagc cagcatggca aaaaggaggc agacccagat gtttgacgaa
      121 aaatgaagtt gggtcagccc aagggcatag gccccgattc cgaagtaggc cccgtgtccg
      181 aaggacatca atcccgtatg accgaggagc atattatagg ctagggcaaa cagggccatg
      241 atcatgattt cggtgcccag gtccgtatag cctccgatga ccggaaaaac gtaaggaagg
      301 atcaggaaga tcagggcaat atagaggatg ggggactgga taattgcctt tgctttctct
      361 ttcaaaattt tcctccttag gcttcaagac cgcttttttc acccatgagc ccgcgcggtc
      421 tcttcaggat gataatggcc aagataacgt acatggccag atcggaaatc cggggagcca
      481 ccatcaccac gatggttacc gtaaggccca cgaggagact tccgatgatc gttcccagga
      541 aacttccgac acccccgacc acgatcacca cgaaggcggg catgatgatc gccatgccca
      601 tatcgggctt cacgccctgg aggggagcga ggagaacacc ggcgatcccg gcgaagatgg
      661 agccgatgct atagaccaag gtgcgaacct tcggaaggtt cttgcccagg gctccgatca
      721 tttcgctgtc actgacgcct gccttgatca ccatgccgta ggtcgtcttc tcgaggaagg
      781 cccacatcag gaagatcagg accagggtga aaaggccggc gaagatgcga tacttcgagt
      841 agagcatgaa tcccaggttg atgcttccca ccaattcacg aggaggattc aaagcatatc
      901 cggtgggccc ccagatcatc cgtatgacct ccgtgctggc gaggaccagc ccgaatgcca
      961 gcagaaggcc gaacaggggg tctttaccgt aaacccgccg aaaggtgagg gtctcataga
     1021 ggatgccgat cagccctacg atgaacggag cgatgatcaa cgagggccag aatccgatgt
     1081 acccggtcag aaccagactg agataggcgc cgaaggtgaa aagggcggca tggacaaagt
     1141 tgatcacctc catgaccccg aggatgaggg tcaatcccaa cgcaataagc gcgtagagga
     1201 gccctaaaat gatcccattg aatagctggc tgaggatata ttccatcgac ctttttctcc
     1261 ttccgaagcc gacagtatca aaatttttga atggacgcta tcggtttccg aaccttcttc
     1321 aggttcttgc ccagagcttc ccgcatagcg gcatctccaa ctgccgcctt actgaccatg
     1381 cccaccatgg cctttcccag taacagccgc aaggccatga tcaaacccca ggttgatagg
     1441 ccagcaaaga cctggcctgg caagcaatta accctcctcc caaggaaagg taggaggaat
     1501 tctctgcccc tagagagctt gccggtatat ttccacgatc gattctacgg ttagctctct
     1561 cgggttcaga tcgacgagcc gtttgttctg gaaagcttct gcggccagca tgggcaggtc
     1621 tttttccttc acccccctct ttcccaggcc gaggggaatg ccgatttttc cgaataagtc
     1681 agagattttt tcgaccagcc ggtcggcaat ctcctggtca cttagtcccc gaatatcgat
     1741 acccatttgc tcgcatattc tcttggcttt ggggatattg cccggggtgt tgtaccgcac
     1801 caccgctggc atcatgaggg catttcccag gccatggggc acgtcaaaat ggccctcaac
     1861 ggggtaggaa gtcgcggcaa tcgcgcctgc tccggccatg agagaaacac ccccgcacaa
     1921 tgcggccagg gccatgtttt ccctggcgtc catgtcattt ccattttcaa ctgcccgttg
     1981 taaattcccg gccaccagct taatggtttg gagagaatac atctcggata gcggattggc
     2041 attcttggac atgaaggatt cgagggcgtg acccagggcg tcgatgccgg tgtgggccgt
     2101 caccccgggg ggcgtggttc gcatgagttc cgcgtcgacg atcgccaccc ggggaaggat
     2161 atgccggctc acgatagctt tcttcgcctt ggctttgagg tccttaaaaa tggcgttccg
     2221 ggttgcttcg gcccccgtcc ccgcggtggt tggaatcagg aaagttggca ctccgggttt
     2281 caggacctgg tcgattccaa aatacttgga gacggaaccc ccattggtaa ccacgaccga
     2341 tgcccctttc gccgtgtcca tcgagcttcc tccccccaca cccacaatca ggtccatctt
     2401 ccccttgcgg gctatttccg cacactggtc aaccagctct aaaggaggtt ccggctttat
     2461 cgagctgaaa atttcggatt tgatccctgc tttttctaat gctccctgca cgcgcgccga
     2521 aatgccggcc tggtggacac cgggatcggt gatgatcaag gctttttgag cccccagttt
     2581 cttcgcctcg ggaatggctt gctccagaga tccccgcccg atccagatcc actcggtaca
     2641 aaaataaaga aacggtgcac cggccatatt tttttctcct ttcaaaaaat ggtatttgct
     2701 gtagtggaaa tcctccccag ttcttccccc aggcccatcc atagggttac ctggatatgc
     2761 atctatctga gggaccggag gtcttcgatc aactcggtca ggcccaactt ctccaacgtt
     2821 tcggaaagag gtttccctcg ctcatcccag cccatcaacc ggaaatagtt ttgcaccatc
     2881 cagggccact tctccaggat atttttcccc ttcgtcggcc cgtcggtcgg ggtcgagccg
     2941 taacggacgg aaggcttttc atcctccatc ctcattccat gcttcaagtt gaagacccgg
     3001 aggagattga cgatgcgtct tccgacggtg aaggcatctt ccagcgtcca attccacccc
     3061 gtgaccgcat tgaagcattc cagcaccagc ttgggatggg gggaggcaat gcggcaggtt
     3121 cccaggcaat catcgaactg ccgaatcccg ttatatttcg cgttc
//
LOCUS       DASPRR010000405         8740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_413337, whole genome shotgun sequence.
ACCESSION   DASPRR010000405 DASPRR010000000
VERSION     DASPRR010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413337"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..599
                     /locus_tag="VEL68_14845"
     CDS             198..599
                     /locus_tag="VEL68_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXZ37297.1"
                     /translation="MNQERKIRVLVAKPGLDGHDRGAKVVAYGLRDAGFEVVYTGLRQ
                     TPEMIVNAAVQEDVDVVGLSILSGAHLSLTQRVLQNLKSKGAGDKMVLVGGIISPADS
                     AKLKEMGVAEVFIPGASIKSIADFIKSKMAP"
     gene            789..2126
                     /locus_tag="VEL68_14850"
     CDS             789..2126
                     /locus_tag="VEL68_14850"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ37298.1"
                     /translation="MKVTEILARYALKASYRSFPKEVVHQAKRCFLDLIGVSLGGSRQ
                     PLAKILLKTVREWGGRPQATIWGHGLKTSVAQAALVNGAMAHALDYDDTHVRAMGHPS
                     APLIPALLAVAEWKGASGKAALEAFIAGFEVETRIGEGMGVKHYNRGWHATATFGRIG
                     AAVAAGKLLGLDLEQMKQAMGLAGTQAAGLRLVFGTMTKPFHPGKSAFDGVLSAVLAQ
                     RGFTCAPNIIEGKKGYLEVLGDDSRLEPMVKNLGKKYEVLNNTFKPFAACLFTHPTID
                     ALIQMRNKYHLKPEDVEEISCDVARFCLDAAGQAEPKTSLAGKFSVYYCAALALAEGV
                     AGEDMFTDRKVIDPRMVALRKKVKARGVPGYKDTEAKVTVTTKGGKTYSIYVDIPKGD
                     PRNPPTDQELEDKFRSLAPFVLSKSKTDRLVKTIWNLEKLESIRQLIRLCHGK"
     gene            complement(2134..4038)
                     /locus_tag="VEL68_14855"
     CDS             complement(2134..4038)
                     /locus_tag="VEL68_14855"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ37299.1"
                     /translation="MAAGKKAAKFLGEAIDRATVGSSILAGIFLLAIAFLTTYEVIVR
                     YAFNSPTHWTLDLSIYLLIWFAYLSIASLQKHRRHVRVDLLITHFSPRTRETWEIATT
                     IIFLFFTGWMIYYVTEYAYSSYRGQEYGWAMWRVLLWPVKAALPVGTVLLGLYLVKDL
                     VSRFHALRSMPQRESGAPLRDNPRVVLPLFLFLVAASIFCYKINGGLGMLCLMLVLLF
                     GGVPIFPALGLTGITGLFLVFGGATAVSSSLPAVSYTSLENFALVCLPLYVFVGQAIS
                     SSGVSDEIYNAATRWVGHLPGGESVATIMACAVFAAVSVSSVATATTIGLIAIPALLA
                     RKYNKSFAYGLLAAGGTLGIMIPPSGTMIIYSAVTEESLGKLFMAGVFPGILLAAAFI
                     IYSMIHCKRTGEYERVAPATWRERFKATKDAMWGLLAPVIIMGGIYSGIFTPLEAGAI
                     AAAYALGMILARRKVTFRQVFKMLGDSTFASTMVLSIIVGAMLLGEFMTLTKIPDQTI
                     EFVSSLNMSPWGVMATLMAIYVILGMFLEVVSIMLITLPIVYPVIIKLGFDGIWFAVM
                     ITLNMEMACITPPVGLNLYAIQGIAQARLADVLKGVWPFFIIMVIWMIFLALFPSLST
                     WLPQVVVPRR"
     gene            complement(4108..5184)
                     /locus_tag="VEL68_14860"
     CDS             complement(4108..5184)
                     /locus_tag="VEL68_14860"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ37300.1"
                     /translation="MKKMILFVCLMTAAVFLPALAGAQPAKGKVVEVRVTGHMPVGQL
                     CTIACERFIKDAETRSQGALKFIHYPAGQLAMDVKALEMCKRGGIEMAQFFVNRAVGL
                     VPETDLTVPYFDDPDWFARRMYDPSSGGGLFYKYLRGKFAQQGLQVMPGFLHSPEHST
                     MTSKPISKMADYRGLKLRSSGRSLSVAMESWGAKSVVMSSADVYMALQRGTIDGANSG
                     LTTFRSRKWFEVINHVQLLWELVASLDMIANLKWWSSLSPDLQKVIEESLRSSAIWSW
                     EEAMKEVEDDIKFLTEKGLKIVDLKKAAPEEWEKMRLANLAAQEKLVGPTVGPEMWKE
                     HVRLLNDTKGSAKTWRQVMQTMKW"
     gene            complement(5181..5303)
                     /locus_tag="VEL68_14865"
     CDS             complement(5181..5303)
                     /locus_tag="VEL68_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37301.1"
                     /translation="MDNQMADVLNFEDFFQPGWNINSGKPSSIGGRLEERRIRS"
     gene            5711..7042
                     /locus_tag="VEL68_14870"
     CDS             5711..7042
                     /locus_tag="VEL68_14870"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ37302.1"
                     /translation="MKVTERIAEYVLESSYRSLPKEVIHQAKRCFLDLIGTTLGGAKQ
                     PLGKILVKTAGEFGGKTQATVVGHGLRTSVLNAALINGSMAHALDFDDTHIASIIHPS
                     APVIPAALAVAEWKRLSGKAALEAFILGFEVETRIGLGMGKKHYDRGWHNTATCGRFG
                     SVVAAGKLLGLNLEQMKMALGMAGTQASGLRLVFGTMTKPFHPGKCAFDGLLSALLVQ
                     RGFTCAPNLIEGPKGYVDVLGVESQLDPMVKNLGKKYEVMNNTFKPYAACLMTHPTID
                     AILEMRNKYRLKPEEVASIDCEVAKFCLDAAGQTEPKTGLVGKFSTCYVAALALAEGA
                     AGEDMFNDKKVVDPKLVALQKKVRAKVVPGYSETEAKVSITTKAGKKYSAFVNTPKGD
                     PRNPPTDRELEEKFRSLAAFVLPKAKIDRLVKAVWNLDKMTNIRQLIRLCY"
     gene            7236..8129
                     /locus_tag="VEL68_14875"
     CDS             7236..8129
                     /locus_tag="VEL68_14875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ37303.1"
                     /translation="MKEKIGFIGLGAMGVPMSQRLLEAGYPLVVYDVRKEAIETIVKK
                     GAQGASSPREVAEKCRKVITIVPNSDAVEQVVFGPGGLLEGMKAGDVLMEMTSSYPPS
                     TLKVNQALAAKGVNMIDAPVSGGVVGAVAGTLSVMVGGDEKIFESCRPILSVMGKNLF
                     YIGGIGFGHALKAINNFLSATTLAATSEAIILASKLGISPQRAIEVLAVSTGRSYTTE
                     LKFPKFVLPRTFNSGFSLELMHKDLNTVTRMAREYKVPMLMANLVQELYGYSLAHGDK
                     KTDHTAIFTSLEELAGTKLKP"
     gene            complement(8458..>8740)
                     /locus_tag="VEL68_14880"
     CDS             complement(8458..>8740)
                     /locus_tag="VEL68_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ37304.1"
                     /translation="IDAQEAFRIGLVNKLVPHEELPKATREFALKFAKTAPIPIALAK
                     KALQNYARLDLIQALDHEQIAQEVCRKTEDRAEGMKSFVEKREPVFKGK"
BASE COUNT         1987 a         2474 c         2511 g         1768 t
ORIGIN      
        1 acgatttaac caaaaattcg aaatcggtac caggaaattc aaaacaaatt cgaatgtcca
       61 aaattcatgt ggtcaaacat gcgttttgaa ttttgaaatc gggatttcgg acttgtttca
      121 ggattcgaca ttcgaatttc ggatttggca ctgaatctta tagaacttat tactcacctt
      181 tttaaggagg cgattcaatg aatcaggaac gaaaaatcag ggttctcgtg gccaagccgg
      241 ggttggacgg gcacgaccgt ggggccaaag tggtcgctta cgggctgcga gatgcgggct
      301 ttgaagtggt gtacaccggc ttgcgccaga ctccggagat gatcgtcaac gccgcggtcc
      361 aggaggacgt ggacgtggtc gggctgagca tcctttccgg ggcccaccta agcctgaccc
      421 aacgggtcct ccagaacctc aagtccaagg gggcgggcga caaaatggtg ctggtgggag
      481 gaatcatctc ccccgccgat tccgccaagc tgaaagagat gggggtggcc gaggtgttca
      541 tcccgggagc ttcgatcaaa agcatagcag atttcatcaa gagcaaaatg gcaccgtaga
      601 gttcgtcatt ccggcgaaag ccggaatcca gaaatcttta aatcccgcct ttggcgggac
      661 tggattccgg gtcgcgcttc gcttgcccgg aatgacaact tccccctgtt ttggatagtc
      721 ttgcaggaac ttctggattt tggaatttat tcggggtttt gaatttcttt tctcaaaggg
      781 agtgagtgat gaaggttacc gaaatcttgg cccgttatgc cctgaaggcg tcctatcgat
      841 cttttcccaa ggaagtggtt catcaggcca agcgctgctt cctggacctc atcggcgtgt
      901 ccctaggagg gtcccgtcag cctctggcca aaatccttct gaagaccgtc cgggagtggg
      961 gaggaagacc ccaggccacg atctgggggc acggtctgaa gaccagcgtc gcccaagcgg
     1021 cattggtcaa cggagccatg gcccacgccc tggattatga tgacacccat gtccgggcga
     1081 tgggccatcc cagcgccccc ctgatcccgg cccttctggc cgtggccgag tggaaagggg
     1141 cttccgggaa ggctgccctg gaggctttca tagcgggatt cgaggtggag acccggatcg
     1201 gcgagggcat gggagtgaaa cattacaacc ggggctggca cgcgaccgcc acctttggtc
     1261 gcatcggggc cgccgtggcc gcgggaaagc tcttggggct ggacctggag cagatgaagc
     1321 aggccatggg cctggccggg acccaggctg ccggcctgcg cctggtcttc ggcaccatga
     1381 ccaagccttt ccacccggga aaatcggctt tcgacggggt cctctcggcc gtcctggccc
     1441 agcgggggtt cacctgcgcc cctaatatca tcgaggggaa gaaagggtac ctcgaggtcc
     1501 tgggcgatga ttccaggctg gaaccgatgg tgaaaaacct tggaaagaag tacgaggtgc
     1561 tcaacaacac tttcaagcct tttgcggcct gcctgttcac ccatccgacc atcgatgccc
     1621 ttattcagat gaggaacaag taccacctca aacccgaaga cgtcgaggag atttcctgcg
     1681 acgtggccag attttgcctc gatgcagcgg gacaggcaga gcccaaaaca tccctggccg
     1741 gtaagttcag cgtttactac tgcgcagccc tagccctcgc cgaaggggtc gccggggagg
     1801 atatgttcac cgatcggaag gtcatcgacc cccggatggt ggccctgagg aaaaaggtga
     1861 aagcccgggg ggttcccggg tacaaagaca ccgaggccaa ggtgaccgtt accaccaaag
     1921 ggggcaaaac ttattcaatc tacgtggaca tccccaaggg agacccgcga aatcctccca
     1981 cggatcaaga attggaggat aagttccgct ctctggcgcc cttcgtcctt tccaaatcca
     2041 agacggaccg cctggtgaag acgatctgga acctggaaaa actggagagc attcgtcaac
     2101 tcatccggct gtgccacggg aaatgaactt tcctcacctc cgcggaacga ccacctgggg
     2161 cagccaggtg ctcagcgaag ggaacagggc cagaaagatc atccagatca ccataatgat
     2221 gaagaaaggc cagactccct tcagcacgtc ggccagcctg gcctgggcaa ttccctggat
     2281 ggcgtagaga ttcaacccca cgggcggggt gatgcaggcc atctccatgt tcagggtgat
     2341 catgaccgca aaccagatcc cgtcaaaccc cagtttgatg atgaccgggt agacgatggg
     2401 cagggtgatc agcatgatcg acaccacttc caggaacatc cccagaatta cataaatggc
     2461 catcagagtg gccatcaccc cccagggaga catgttcagg gaagagacaa attcaatggt
     2521 ctggtcgggg attttggtca gggtcatgaa ttcgcccagg agcatggccc ccacgataat
     2581 actgaggacc atggtgctgg caaaggtgga gtctcccagc atcttaaaga cctgccggaa
     2641 ggttaccttc cggcgggcca ggatcatccc cagggcgtag gccgcggcga tcgccccggc
     2701 ctccaggggg gtaaagattc ccgaatagat tccccccatg ataatgaccg gagccaggag
     2761 tccccacatg gcgtctttgg tggccttaaa cctctcgcgc caggtggctg gggcgactct
     2821 ctcgtattcc ccggtccgct tgcagtgaat catcgaataa ataatgaagg ccgcggccag
     2881 gaggatgccg ggaaagactc cggccatgaa aagcttgccc agggattctt cggtgaccgc
     2941 agaatagatg atcatggtcc cgctgggggg aatcatgatt cccagcgttc cccccgcagc
     3001 caacaggccg taggcgaaag atttgttgta cttgcgggcc aggagggccg gaatggcgat
     3061 cagcccgatg gtggtggccg tggccacgct ggagaccgac accgcggcga agacggcgca
     3121 ggccattatc gtagctacgc tctccccccc gggaagatgg cccacccacc gggttgcggc
     3181 gttgtagatt tcgtcgctga ccccgctgct ggaaatggcc tgccccacga agacgtagag
     3241 gggcaggcag accagggcaa aattctccag cgaggtgtaa ctcaccgccg ggagggaaga
     3301 gctgaccgct gtcgccccgc cgaaaaccag gaagagcccc gtgattccgg tcaaccccag
     3361 ggcgggaaag atggggaccc ccccgaagag gagcaccagc atcaggcaga gcattcccag
     3421 gcccccgttg atcttgtagc agaagatgct ggccgccacg aggaagagga agaggggcaa
     3481 tacaacccgg ggattgtccc ggagaggagc ccccgattcc cgctggggca tggatctcag
     3541 agcatggaac cggctgacca ggtctttgac caggtacagc cccaggagaa cggtcccgac
     3601 gggaagggcc gcctttaccg gccagagcag gacccgccac atggcccagc cgtattcctg
     3661 gccgcggtaa gaggaatagg cgtactcggt gacatagtaa atcatccacc cggtgaaaaa
     3721 tagaaagatg atcgtcgtgg cgatctccca ggtctcccgg gtgcggggag aaaaatgggt
     3781 gatcagcagg tccacccgca catggcggcg gtgtttctgg agagaagcga tggacaggta
     3841 ggcgaaccag atgagcaggt agatggaaag gtccagcgtc cagtgggtgg gggagttgaa
     3901 agcgtagcgg acgatgactt cgtaagtagt aaggaaagca atcgccagga gaaaaatccc
     3961 ggccagaatg ctacttccga ccgtcgcccg gtctatggcc tcccccagaa attttgccgc
     4021 tttctttcca gcagccatgg gccctcccgg cgtttttggc cgtccctgcc atgcaagggg
     4081 ctgaggcctc agccccctct tcacccgcta ccacttcatg gtctgcatca cctgccgcca
     4141 ggttttcgcg gaccccttgg tgtcgttcag caggcgcaca tgttctttcc acatctccgg
     4201 gcccacggtg ggccccacaa gtttttcctg cgcagccagg ttggccagcc gcatcttctc
     4261 ccattcctcg ggagcggcct tcttgaggtc cacgatcttt aaacctttct cggtcaaaaa
     4321 cttgatgtcg tcttcaacct ctttcatggc ttcttcccag ctccagatgg ccgacgaccg
     4381 gagggattct tcgatgacct tctgcaaatc cggagaaagg ctgctccacc atttcaggtt
     4441 ggcgatcata tccagggagg ccaccagctc ccacagcagc tgaacgtggt tgatgacctc
     4501 gaaccacttg cgggaacgga aggtggtgag gccgctgttg gccccgtcga tggtcccgcg
     4561 ctgaagagcc atgtaaacat ccgctgaact catgaccacc gatttggccc cccagctctc
     4621 catggcgacc gaaagggacc ggcccgaact cctcagcttg agcccccggt aatcggccat
     4681 cttggaaatg ggcttgctgg tcatggtgga atgctcggga ctgtgcagga acccgggcat
     4741 gacctgcagc ccttgctggg cgaattttcc cctcaagtat ttatagaaga ggcccccgcc
     4801 gctggaagga tcgtacatcc ggcgggcgaa ccagtccggg tcgtcaaaat agggaacggt
     4861 caggtcggtc tcgggaacga gtcccaccgc ccggttgaca aagaattgag ccatctcgat
     4921 cccgccccgc ttgcacatct ccagggcttt cacgtccatg gccagttgtc ccgccgggta
     4981 atggatgaac tttaaagccc cctggctccg ggtttcggcg tctttgatga acctctcgca
     5041 ggctatggtg caaagctggc ccacgggcat gtggccggtg acgcggacct ccacgacctt
     5101 ccccttcgcc ggctgagccc cggccagggc cggcagaaag actgccgcgg tcatcaggca
     5161 gacgaacaat atcattttct tcatgacctt atcctcctct cctctaatcg acctccgatg
     5221 ctggaaggct ttccagagtt aatattccac cccggctgga aaaaatcttc aaagttcagg
     5281 acatccgcca tctgattgtc catcgggtcc tcctgccgga tcggcttata tgatttcctg
     5341 acgaccgcgt gtaccacgca gaaggttcca ggtaacggct ttacctggtg gggcgattca
     5401 tgaatcgccc cgatggtcag cctgcgcaat ttccaacacg cttcgtaaaa aaataacggg
     5461 gaacattggc tagcaaaaga agggtccagg cccaaaataa gagttttcaa tttttctgtc
     5521 aagaaaattc attctttttc attcattcgc actattttct tccttcgaat tcgaggttct
     5581 tcgcctcttc ggtgcgggaa acagagccat ttaaaattgg caggggattt gttactatag
     5641 actcgacatc caccgcacgg gcgtataagt tgccctgcag aattttcggg gtgaataaag
     5701 gaggaacttg atgaaggtta ccgaaagaat cgctgaatat gtattagaat cttcctaccg
     5761 ttccttgccc aaagaagtga tccaccaggc caagcgctgc ttcttggacc tcatcggcac
     5821 gaccctggga ggggccaagc aacccctggg gaagattctg gtaaaaaccg ctggcgaatt
     5881 tgggggaaaa acccaagcca cggttgtggg ccatggatta agaaccagcg tcctgaatgc
     5941 agctctcatc aacgggtcta tggctcacgc cctggatttc gacgacactc atattgcttc
     6001 catcatccat cccagcgccc cggtgatccc cgcggctctg gccgtggccg aatggaagcg
     6061 gctttcaggg aaagccgcac tggaggcctt catcctgggc ttcgaagtgg agacccgcat
     6121 cggcctaggt atggggaaaa aacactatga ccgaggctgg cacaatactg cgacctgcgg
     6181 ccgcttcggg tcggtcgtgg ccgctggaaa gctcctggga ctcaacctgg agcagatgaa
     6241 gatggccctg ggaatggccg ggacccaggc ttcgggcctt cgcctagtct tcggcaccat
     6301 gaccaaaccc ttccatcctg gaaaatgtgc cttcgacggc ctgctctcgg ccctcctggt
     6361 gcagcgcggc tttacctgcg ctcccaacct catcgagggc cccaaaggct atgtggatgt
     6421 cctgggagtc gaatcccagc tggaccccat ggtgaaaaat ctgggcaaga aatacgaagt
     6481 gatgaataac accttcaagc cttacgcggc ctgcctgatg acccacccca ccatcgacgc
     6541 cattctggag atgcgcaaca agtatcggct caagccggaa gaggtggcgt cgatcgactg
     6601 cgaagtggcc aaattttgtc tcgacgctgc tgggcaaaca gaacccaaga ccggcctggt
     6661 cgggaagttt agcacctgct acgtggccgc gctggcgctg gccgaaggag cggccggaga
     6721 agacatgttc aacgacaaga aggtcgtcga tcccaagctg gtggccctcc agaaaaaggt
     6781 ccgggccaaa gtggtgccgg gctattctga aaccgaagcc aaagtctcca tcaccaccaa
     6841 ggcgggcaag aagtattctg ccttcgtgaa cactcccaag ggagacccac ggaatccccc
     6901 taccgacagg gaattggagg aaaaattccg ctccctggcg gccttcgttc tgcccaaggc
     6961 caagatcgac cggctggtga aggcggtctg gaatctagat aagatgacca acatccggca
     7021 gcttatccgg ctttgttatt agcgatctct ctgacagccg ttgtttcttc gtaggggcgg
     7081 gtcgcgaacc gcccctacat gggctttgca atctgcatgt ttccttctga gcaaaatgtt
     7141 ggtattcgtt tagcattaag cgtctaaaag ccctaatcag aggggaaatc cccgggcaca
     7201 agctttcgca tagacaaaga tgaagggaga acgggatgaa agaaaaaatt ggctttatcg
     7261 gtttgggcgc tatgggcgtg cccatgtctc agaggctatt ggaagctggc tatcctcttg
     7321 tggtctatga tgtaagaaag gaagcaatcg agaccatcgt aaaaaagggg gcccagggag
     7381 cttcttcccc ccgagaggtg gcggagaaat gccgcaaagt catcaccatc gtccccaact
     7441 cggatgccgt ggaacaggtg gtattcggcc ccggagggct cctggagggg atgaaagcag
     7501 gggatgtgtt gatggaaatg accagctctt atccgccgag cacgctgaag gtcaaccagg
     7561 ccctcgctgc caaaggcgtc aatatgatcg atgctccggt gagcggcgga gtggtgggag
     7621 ccgtggccgg aactctctcg gtcatggtgg gaggggacga aaaaatcttc gagtcctgcc
     7681 gccccatcct ctcggtcatg gggaagaatc tcttttacat aggaggaatc ggtttcggac
     7741 acgccctgaa ggcgatcaac aacttcctct cggccacgac cctggctgcc acttccgaag
     7801 ctatcatcct ggcgagcaaa ttggggatct cccctcagag ggcaatcgag gtcctggcgg
     7861 tcagcaccgg ccggagctac acgacggagc tcaaattccc caagttcgtc cttccccgga
     7921 ccttcaactc cgggtttagc ctggagctca tgcacaagga ccttaacacg gtcacccgca
     7981 tggccaggga atacaaagtc cccatgctta tggccaacct tgtgcaggag ctttatgggt
     8041 actccctggc ccatggagac aaaaagacgg atcacaccgc catctttacg agcctggaag
     8101 aactggccgg aacgaaattg aaaccctagc caaaaatatt tggagttgcc atccgatccc
     8161 ctcccccttt ggcgaggaag ctttgaagaa attgaattct ccccctcacc ctaccctctc
     8221 ccgcgagggg agagggtaaa catattgaaa tagaaaaaaa tccctccccc ttgagagact
     8281 gtgttgtaat ccgttcatgg ttcgacaagc tcaccacgaa cggattatga gcgcattaaa
     8341 attcagccac ttagccgttc gccctgagcc tgtcgaagga cgaacggggg attacgacac
     8401 agtctctgac gggggagggg cagggtgggg gtggaaatgg gattatttca tactttctca
     8461 tttccccttg aacacaggtt cccttttctc tacgaacgat ttcatgccct cggcgcggtc
     8521 ctcggttttg cggcagacct cctgggcgat ctgctcatga tcgagggcct ggatcaagtc
     8581 cagccgcgca taattctgca gggctttctt ggccagggcg atggggatgg gggcggtctt
     8641 ggcgaacttc agggcaaact cccgggtggc tttaggcagt tcttcgtggg ggaccagctt
     8701 attgaccaag ccgatccgaa aggcttcctg ggcatcaatc
//
LOCUS       DASPRR010000406        16020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1710189, whole genome shotgun sequence.
ACCESSION   DASPRR010000406 DASPRR010000000
VERSION     DASPRR010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16020)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16020
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710189"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VEL68_14885"
     CDS             complement(<1..560)
                     /locus_tag="VEL68_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37305.1"
                     /translation="MKRREFLKTGAAVLGTSVIADKFFSLGSGARLAQAAPTGAEGGN
                     RRFATCLGPYNTKGLNVTMPEEVILSDVTMRDGEQTVGVAFSLEEKVDLARRLSALGI
                     PQFQLGRADTPDGRREAETLCHLGLPCKIEIMAGGGARDWRQQIDAALACGADIVHTN
                     IATSTYMRNMQGDIPESEVIKRLNDVV"
     gene            complement(1204..1866)
                     /gene="budA"
                     /locus_tag="VEL68_14890"
     CDS             complement(1204..1866)
                     /gene="budA"
                     /locus_tag="VEL68_14890"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HXZ37306.1"
                     /translation="MLQISTVTGLERGLFYPVTTVGWMKQNGNIGLGAFEGMDGELLI
                     LDGKAYNAMYSGEVVAVEDNSPIVYGAVAQLSADRTESLQNIASYEQLQKSLEQLLPN
                     KNIFYVFKVQAAFNYLKVRSTPKQKLPYTGLADVVKNQSIFEFKDIKGTLIGFRCPAY
                     IQGVYAPGFHLHFISDDRQKGGHVLEANVAELTAQIGYLTQMYLVLPDNEATRKMDMS
                     AK"
     gene            2960..3433
                     /locus_tag="VEL68_14895"
     CDS             2960..3433
                     /locus_tag="VEL68_14895"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ37307.1"
                     /translation="MRRRDLISGSAFLVLAILLFFQAKKLTLWGELGPAEGFFPMGLS
                     LVFAGLSVLILIQALLKPVEAGERVGIIDLNRKKFFAYVGLFFGFGILFPIVGYTLIL
                     AAFLVIILRWVEKQSWKITILVTILLIGGSNLLFVGILKISMPEGLLSSAFSLWP"
     gene            3442..4935
                     /locus_tag="VEL68_14900"
     CDS             3442..4935
                     /locus_tag="VEL68_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ37308.1"
                     /translation="MLDNLLLGFQISLSFANLFYCLVGVTLGTVVGVMPGLGPAATIA
                     LLLPLTFNMNVTAAVIMLAGIYYGVAYGGTITSVLVNIPGEACTVVTCIDGYQMARKG
                     RAGAALGIAAFGSFFGSTFGIIGISLLAPPLASLALTFGAAEYASLMIAGMTLVTYLS
                     SKSVLKSLTMAVLGMVLGCIGLDPIHGTERFTFGTLVLLDGINIAPLAMGLFGIAEVL
                     QIAEGFVKQDGLVQKSVKIRNLLPNRQDWKDSIGPIGRAIPLGFFLGILPGGGAVMSS
                     FLSYALEKRISKHPERFGTGTIEGVAGPETANNSGTAGAFIPLLTLGLPFNVITALLL
                     AAFMVHGVSPGPLILRKHPDIFWGVVTSMYVGNFLLLLLNIPLIGLFINILRIPYSIL
                     SPLIAVVCFVGAFSVNNNATDVVVMVFFGVVGYLMRKFEYDAAPLLLAYVLGPMLERS
                     LRQALIISDGSVAFFFQRPISAALLAVAFISLATPLWKWVANQYKSG"
     gene            4983..5948
                     /locus_tag="VEL68_14905"
     CDS             4983..5948
                     /locus_tag="VEL68_14905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37309.1"
                     /translation="MRKTKLICTLALGCLLALSTICEAASVFPSKPISVYCPWAAGGS
                     TDLTMRMLANQATKILGQPVVVVDKAGGGGFIANAEAFKAAPDGYTLVVNASSTLTLS
                     PLLRKAPFDPRKMTPILSYGVYPFTLAVRSDAPWKDIKEFVEYMRKKPGEIKMSTSGP
                     DAMENLAMYMLKEQEKLDFKLVPYEGGAPAVAATLGGHVQAFIGVAEAIPHIRDGSMR
                     GLATFLSERMPGMPDIPTLRESGYNVVVESRLAIYGPPGIPQDTVKKLEAAFKQAMEN
                     EDFKKVCKTFEVTPSFIGSEGIEKYHSDLTGRIKGTLIKIGRIKD"
     gene            6056..7174
                     /locus_tag="VEL68_14910"
     CDS             6056..7174
                     /locus_tag="VEL68_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ37310.1"
                     /translation="MKIKRIEIIPVTIPLKKKVAISFITWEKVEYVITKFYTDEGIVG
                     IGESAPWAPISRESQETVVGLASKHLAPLLIGEDPFDVEKIWWKMDTVIPGMGMAKAA
                     LDLPLYDIMGKALNVPVYKLLGGKATETFPLVGLVGLGTVKEMIDETMDWIRQGYRSM
                     RIKTGRGLREDVQSIKAIREAVGPEIRLRVDANQAYSTPMAIRVIKALEPYGIEIAEQ
                     PTAWYDFEGLARVTAAVDTPVMPHESLYSIYDAIQLDRMGAGNVYGLKIYRPGGVTLA
                     KKLATYMELRNIPMFVCSCVELAVSTAASAHFAVASYRNIKFASEMSGPVGLEDDIAE
                     SAVQIKDGMAAIVDRPGYGVELDEKRMKKYGGEIITIK"
     gene            7539..8693
                     /locus_tag="VEL68_14915"
     CDS             7539..8693
                     /locus_tag="VEL68_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ37311.1"
                     /translation="MKISSIELVNVNIPLAPSDLPKPVGRNYGCFMLVRIKTDKGLEG
                     IGDGYYGNSAPGVAATIRETLTPEIMGREATAVGGLYERMYRAGFYSGRIGIYSYAVS
                     AVEMALWDLLGKYHGVPVYELLGGMVSRAVAPYPTLRAVMDEKEEKKVPVYASLQTFR
                     TAEEVGLIAKAAVEEGFKSVKLHQVDIPSVRVTREAVGGGIEVTMDVNGVFNSLEAER
                     FARQLAEFDIGWFEEPIWPPDDYRALAQLRRRSPVPIAGGENESTVYGFERILETEAV
                     DVLQPEILSVGGLLEMYKVLAMAQARSLPVAPHNFKFGPVLAATLNLSLLSSNVFTTE
                     TPWFKLEANLFKKGPQIRNGWACLSDLPGLGFEIDEEVVKEYRIEKFPQK"
     gene            complement(8823..10007)
                     /locus_tag="VEL68_14920"
     CDS             complement(8823..10007)
                     /locus_tag="VEL68_14920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ37312.1"
                     /translation="MPQPLANIRVIDATSAISGPYCSMLLGDMGAEVIKIEEPSEGDL
                     LRKSGPFVGGESTYFLYGNRNKRGMTLNLRESAGRDILLKLVKGADVLVENFRPKVKS
                     RLRIDYPALSSINPRLIYCSISGFGQTGPWAETPGFDQIAQGMSGLMSVTGFSETGPT
                     RVGVAIGDSLCAIFAAYGILGALLEREKSGKGQFIETSLLEALIAVLGFQAAKYFGTG
                     EAPLPQGNDHASLAPYGTYKTLDGYINIAAGTSRMWEELCRQLGVERLLKDDRFMSNP
                     NRVANKEELRRILESRLVTERTDHWVESLGKAGIACGPIYTLSQVFHDPQVLNQKMLL
                     ETEHLTAGKISLIGFPVKLSRTPCSISLPPPTLGQHTEEILKELEFSKEEIRALREKR
                     II"
     gene            complement(10028..11053)
                     /locus_tag="VEL68_14925"
     CDS             complement(10028..11053)
                     /locus_tag="VEL68_14925"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ37313.1"
                     /translation="MKDFRSAMKMAILFLLFFCLCLEVPSALAASKPEFTLRMAGTMP
                     IGHFMTRAKERYSQILSEKTGGRLKIEVYPAGQLFSDKDMTRALPAGSVEMGACNLAL
                     LEGLVPVLGIMGLPYFFESREHIRKVEHSKSFVQLINDEMEKKNMKLLFWMDYGLMSF
                     ISKKPLQKLEDFKGKRIRVLGDITTQLIQAFGGAPVFMGTGETYMALQRGTIDSVLTS
                     LPSVYERKLFEVAKQMTYCEIQDILVTPAVLINLKIWKGLPPEIQKEMYEVGQEIEEW
                     GFQESLKETEECLSELKKRGVEVYYLPAMEKKRWKEAANKPILGYYLKQVGDPGKKAV
                     EEVDRLR"
     gene            complement(11103..12989)
                     /locus_tag="VEL68_14930"
     CDS             complement(11103..12989)
                     /locus_tag="VEL68_14930"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ37314.1"
                     /translation="MQSTASPESFFNRMVIAIGLLGGALLVCVAGMVTYEVLMRYFFN
                     APTTWVMEISIYFSLAGIFLSLAFVLKGKAHVRVDFVINRLPPRTTISLEIITSLLAI
                     VYCGVMAWEGMKLVLNSFRLGEVSPTILKVPMFIPQVFIPVGSTLLGVTFVKYIRDLF
                     SHLADGKGKDAAESSGGSQMAICGGFAVLLVFSLFLLGVSMNLGLLVLFFVLLLSGIP
                     VSIALGLFGMFGFYFLFGGPRMLSQLPVMAYSTMDNYVMVALPLFILTSTILNRGEVG
                     VRIYRFANVLVRHLPGGLGIASIIFCGLFAAMTGSSVAVAAAVSLIALPEMLARGYPR
                     TLTIGLLAAGGTLGILFPPSVAMILYASMTNESLGSLFMAGLIPGLILSLGFCAYVAF
                     VGGRNKEIQRDRRATLGEILQATRDASGGLITILIIIGGIYSGIFTPTESGGVASIYS
                     LVLCALVYRSFNAQGIRSILLDTARVSGMILFIIIGANITGQVILMSQISQKILLAVK
                     SMELPGWVIILSINVFLIIAGGPLEAISILVITLPILYPLVTGLGFSGLWFAVIMVIN
                     MELALISPPEGLNLFVLQNLAKSNAAEVSRSVIPFLVIIGLFLVLISSVPALTTWLPQ
                     VLGP"
     gene            complement(13315..13863)
                     /locus_tag="VEL68_14935"
     CDS             complement(13315..13863)
                     /locus_tag="VEL68_14935"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXZ37315.1"
                     /translation="MLNEVENYLLVLEHLRGQVRDLLVKFSAESLDWRPLEGTGDLAT
                     NSPAALVTHLAGSENFLIAHVIGGRPIQRDREEEFRTKGVEVAELLRRLSAGTGMAEE
                     ILSPLTPAQLEESRKWLTGSGTVRRCILHAISHYAAHLGHMQLTYQLWVDQVGKVQLA
                     SNPDRIAAGRETSTEGRGKKGK"
     gene            complement(14345..15022)
                     /locus_tag="VEL68_14940"
     CDS             complement(14345..15022)
                     /locus_tag="VEL68_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HXZ37316.1"
                     /translation="MFFNPLTFLLMIFFLFIVLFFFLMVEVNVIALVFAKIGIPPRHI
                     FASLFLILIGSFINIPIKKFPQETMINERSVSFFGIRYRIPPAEKPETVLAVNLGGAV
                     IPCLLSFYLFFKTGLWVKGLAVTAIMVFVTHRLAKPIKGVGIAMPAFIPPIIAAVFSV
                     IFSYDYAPVLAYIGGTLGTLIGADILNVSKIKNLGAPVASIGGAGTFDGIFLSGIMAV
                     LLAAILT"
     gene            complement(15324..>16020)
                     /locus_tag="VEL68_14945"
     CDS             complement(15324..>16020)
                     /locus_tag="VEL68_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXZ37317.1"
                     /translation="EQALLRFVSSPKSAGPLEVGCGTGHWLDLLSKNGRIPVGVDPST
                     NMLLRAREVSSDRALVRGRAENLPFPNGCFDRVFCINAFHHFADKSAFLFEARRVLRP
                     SGGLMMIGLDPHSGQDNWWIYEYFPETVKLDKERYLPTAEIKAAMGRSGFISCEVEEV
                     EHITATLPARLAASEGLFERGFTSQLTILSKGEYEAGVSRLNRAIDQAAAKGEDFLLT
                     TDLRLFGVFGWIG"
BASE COUNT         3806 a         4193 c         4306 g         3715 t
ORIGIN      
        1 acgacgtcat tgagccgctt gattacttcc gattccggaa tgtcgccctg catgttccgc
       61 atgtacgtgg aagtggcaat attcgtatgc acgatatccg cgccgcaggc cagcgccgcg
      121 tcaatttgct ggcgccaatc cctggccccc ccaccggcca tgatctcgat tttgcaaggg
      181 agaccgaggt gacacagggt ttccgcttct ctccggccat ctggggtatc agcgcggccg
      241 agttggaact ggggtatccc gagggcgctg aggcgccggg ctaaatccac cttctcctca
      301 agcgagaatg ccacgccgac ggtctgttct ccatcgcgca tcgttacatc ggagagaatc
      361 acctcttccg gcatggtgac atttagcccc ttggtgttgt acgggcctaa acaggtggcg
      421 aacctcctat tcccaccttc cgcccctgtc ggggcagcct gagccagtct tgccccggag
      481 cccaaagaaa agaatttgtc agcaatcacg gatgttccca ggacggctgc cccggtcttc
      541 agaaactctc tccgcttcat gttattcctc ctttaaaatt acataccgct gccgtatttt
      601 agttattgat taaaaaatcg gcaggcacct ccaaaaggag atcttaagcc ctttggagcc
      661 ttcacataag ggcattctgc aaaacccatt tcataatcct ccaggataaa tcaaatggtc
      721 ttgcttttag gaaggccaag ttgagcgaat tttagcaggg tgggaaagtt tgtcaagcgg
      781 ggctttgaaa taaggtggag gtctgatccc ggactcaggg tcgttaggaa atccacaaaa
      841 tattgggggg tgttcaagcc atctaaaggt tgcgagtgcg gtaactcttt gagcgaaagc
      901 gtcttcaggc aacatagcag ggtgcgcaac ctttcacccc aaaacctacc ccccaacata
      961 tgggcatgaa aaaaggcaga accatgaggg ccctgccttt ccgaggtagt atttggattt
     1021 ctaatgggca atgcaaacgt ttctattgtc ccggggaagt ctataatctt ttgtcacctc
     1081 atagcgcctg gaacagcttc cgcttcaatt ccagacaaat cccgcaaaat ttgatagccg
     1141 aagatcaaca tttatcaagt aacaaaatgt tgtccctgag agatatcatt cagggaatgt
     1201 accctacttt gctgacatgt ccattttcct ggtcgcttca ttatcgggca gcactagata
     1261 catctgcgtc aggtacccga tttgcgcagt cagttcggcc acgtttgctt ccagcacgtg
     1321 cccgcctttc tgacgatcat cactgatgaa atgcaggtga aaaccaggag cgtagacccc
     1381 ctggatatag gctgggcagc ggaatccgat cagcgttccc ttgatatcct tgaactcaaa
     1441 aatggattga ttctttacca catcggcgag cccggtatac ggtaattttt gtttaggggt
     1501 actccgtact ttcaagtaat tgaatgcggc ttgaacctta aaaacataga agatattctt
     1561 gttggggagc aactgttcaa ggcttttctg aagctgctca taactggcga tgttctgtag
     1621 agattcggtg cggtcggcgc tcaactgggc gaccgccccg tagacgatgg gagaattatc
     1681 ctctaccgcc acaacttccc cggagtacat ggcgttatag gccttgccgt caaggatcag
     1741 cagctccccg tccattcctt caaaagctcc aaggcctata tttccgttct gcttcatcca
     1801 cccaacggtc gtcacgggat aaaataatcc gcgctccaac ccggtcactg tggatatctg
     1861 aagcatagta cccttgggcg ccgatacctg cgcataacca ggcatgctcg ccacacacaa
     1921 aaagatcacc aacaggcacg cacccaacaa tcctcttgtc cagcgatgat tcaaatacat
     1981 gggttatctc ctctcaggtt tttgggcggg acagataccg ggtgcctcct tccgttatgg
     2041 agaccggaga tcctggcaga tcagtcttcc cgcctttttt tcttcgccaa tgtccgatct
     2101 ctatctaaaa agcaaacccc catttctctt aaaaaaggtg gttcgctatg ttttccactt
     2161 atagcaacgt ctctctcttt gtccggctcc ggatcattct cctcggccag acccaactga
     2221 tcagatttta taggaaaatt cctggaagat caagactcat tccgcaacag ggcaaaattt
     2281 agcactaaga aataccacag aggtgccgaa ttccctactc agcttctcaa cggcgatgcg
     2341 aagaaccttg ctaccatcca ggacctcctg ggccatggtc agattaccac gactcagcgg
     2401 tactgctgaa cccacggatt ctctctcaaa acaactaatt atcttgacag agaataaaaa
     2461 aataaattaa atagctgcat aactaaccaa tattcgggct gggctccata tatcttgtgg
     2521 ccagtggcga tgatccgggc cctactgcag gaagtaaatg gacgcgttcc caatatatgc
     2581 ggtcgggagg atggatactg ggccggggct cccgagcgca ttgggcggaa gccataagta
     2641 cgagcccttg gccggcgcga tgtttgggcc gggggcggtc tggagcgcgg ccccggcata
     2701 gagattgtcg ttctgtccga ggtaaccctg gtctgtagct ggggtgtaat ggttgtttgc
     2761 aattgagtcg gtaacgacga atgctacgag tgcaaatctg tgctagaatg gaaagccaga
     2821 tgctggattt ggataacaaa ccacgagagg ttatccagag aaggaaagga tattaggaga
     2881 tttcatcata atcctggggc agcgaatcgt aaatgattcg gtttgctgct taacttaaaa
     2941 gaaaagcggg agggatgggg tgcgccggcg agacctgatt agcggatccg cttttttggt
     3001 tctggcgatc cttctatttt ttcaggccaa gaaacttacc ctctggggcg aactgggccc
     3061 ggccgaaggc tttttcccca tggggcttag tctggttttt gcaggactca gcgtattgat
     3121 ccttatccag gcccttctca agccggtcga agctggggaa cgagtcggga ttatagacct
     3181 gaacagaaaa aaattctttg cctacgtagg tctcttcttc ggctttggga tcctgtttcc
     3241 tatcgtgggt tataccctca tcctcgctgc attcctggtc atcatcctac ggtgggtgga
     3301 gaagcagtct tggaaaatta cgatcctggt aacgattttg ctgattgggg gctctaacct
     3361 cctgttcgtc ggaatcctga aaatttccat gcccgagggc ctgctttcct cggcgttcag
     3421 cctctggcca taaggtcgaa agtgctcgac aaccttctcc tcggctttca aatttctctt
     3481 tcctttgcca atttatttta ctgcttagtg ggggtcacgc tgggcacggt tgtgggggtc
     3541 atgccgggtc tcggcccggc cgcgacgatc gccctgcttt tgccgctgac attcaatatg
     3601 aatgtcaccg cggcagtcat catgctggcg ggtatctatt acggggtcgc ttacggagga
     3661 acgatcacgt cggtgctggt caatatcccc ggggaggcct gcacggtcgt gacctgcatc
     3721 gatgggtatc agatggcccg aaaggggagg gctggtgccg ctctgggcat tgcagccttt
     3781 gggtccttct tcggtagtac cttcggcatc atcggcataa gcctcctggc gccgcctttg
     3841 gcctccctgg ccctgacctt tggcgctgcg gagtacgctt cgctgatgat cgcggggatg
     3901 acgctggtta cctatctttc cagcaagtcg gttctgaagt ccttgaccat ggcggttttg
     3961 ggaatggtcc ttggttgcat cggtctggac ccgatccatg gaaccgagag gttcaccttt
     4021 ggcaccttgg ttctcctcga cggaatcaac atcgccccgc tggccatggg actctttggc
     4081 attgccgagg tgctgcagat cgccgaaggg ttcgtcaagc aggacgggct ggttcaaaaa
     4141 tcagtaaaga tccgcaacct gctgcccaat cggcaggact ggaaagactc aatcgggcct
     4201 atcggccggg cgatcccatt ggggtttttc ctcggcatcc tgcctggcgg gggagcggtg
     4261 atgtcttcct ttctgtctta tgcgctggag aagaggatct cgaagcaccc ggaaaggttt
     4321 ggcacgggaa cgatcgaagg ggttgccggg ccggagactg caaacaattc ggggacggcc
     4381 ggtgccttta ttccgttgct taccctgggg ttgcccttca acgtcattac cgccctcctt
     4441 ctcgcggctt ttatggtcca cggggtgagc cctgggccgc tgatcttaag aaagcacccc
     4501 gatatttttt ggggagtcgt caccagcatg tacgttggta acttcctcct cctgctgctc
     4561 aatatccccc tgataggcct cttcatcaat atcctgagaa ttccctattc cattctttct
     4621 ccattgatcg ctgtggtctg tttcgtgggc gccttcagcg ttaacaataa cgcgacggat
     4681 gtggtggtga tggtcttctt tggggtcgtg ggatacctga tgcgcaaatt cgaatacgat
     4741 gcggcgccct tgctgttggc ctatgttctg ggccccatgc tggagcgctc gctgcggcag
     4801 gcgttgatca tctctgacgg aagcgtcgcc ttttttttcc agaggcctat aagcgctgcc
     4861 cttctggccg tggcttttat ttctctggcc accccgctgt ggaaatgggt ggccaaccag
     4921 tataagagcg ggtaagaggc agagcaaacc ccagtaaaag aaggaaacga aaggagaacc
     4981 tcatgcgtaa gacgaagctg atctgtacct tggctcttgg atgtctgctg gccctctcga
     5041 ccatctgcga agcggcatcc gttttcccct caaagcccat ttccgtttac tgcccgtggg
     5101 ctgcgggtgg ctcaacagac ctgacgatgc gaatgctggc caaccaagcc accaaaatcc
     5161 ttgggcaacc ggtggtcgta gtggacaaag ccggcggcgg gggttttatc gccaacgccg
     5221 aagcttttaa ggcagcaccc gatggatata cgctggtcgt aaacgccagc agcacgctga
     5281 ccctatcccc gctgctgcgg aaagccccat ttgatcccag gaagatgacc ccgatcctat
     5341 cttacggtgt ctatcccttc accctggcgg tgaggtctga cgccccttgg aaggatatca
     5401 aagaatttgt ggaatacatg agaaagaagc cgggggagat caagatgtcc acctctggac
     5461 ccgatgcgat ggaaaatctg gccatgtaca tgctgaaaga acaggagaag cttgatttca
     5521 aattggtccc ctacgaaggg ggtgccccgg ccgtggcagc caccctggga gggcatgtcc
     5581 aggccttcat cggagtggcc gaggcgatcc cccatatccg ggacgggtcc atgcgcggcc
     5641 tggctacctt cctgtcagag aggatgcccg ggatgccgga tatccctacc ctaagggagt
     5701 ccggctataa cgtcgttgtc gaatcgcgat tggcgatata cgggcctccg gggattcctc
     5761 aagataccgt caagaagctg gaagccgcgt tcaagcaggc gatggagaat gaggatttta
     5821 agaaggtgtg caagaccttt gaagtcaccc cttccttcat tggcagcgag ggaatcgaga
     5881 aatatcacag tgatttgacg ggccggatca aggggaccct gatcaaaatc gggaggatca
     5941 aggattaaat gagtatatcg cgagagaaga attgctgata accgaatgga aaaacccctt
     6001 gccctatgtc tgaagggagg ataggggcat agaaaaaaac caatagggag atcccatgaa
     6061 gatcaagaga atcgaaatta ttcccgttac catcccattg aaaaaaaagg tggccatttc
     6121 ctttatcacc tgggagaagg tcgaatacgt catcaccaaa ttctatactg atgaagggat
     6181 cgttggaatc ggcgaatcgg ccccttgggc gccgatctcc cgggaatcgc aggagacggt
     6241 ggttggtctc gcctccaaac atctggctcc cctgttgatc ggagaagatc ccttcgacgt
     6301 cgagaaaatc tggtggaaga tggacacggt catccccggc atggggatgg caaaggcggc
     6361 cctggacctt ccgctctacg acatcatggg taaagccctg aatgtgccgg tttataaact
     6421 tttgggtgga aaggcaaccg aaacttttcc cctcgtgggg ctggtcggac tgggtacggt
     6481 caaggaaatg atcgacgaga cgatggattg gatcaggcag ggttatcgca gcatgcggat
     6541 caagacaggc cggggtctgc gggaggatgt gcagagcatc aaggccatcc gcgaggccgt
     6601 aggaccggaa attcgtttgc gggtcgacgc caaccaggcc tatagcactc ccatggccat
     6661 ccgggtgatc aaagcactgg agccttatgg gattgagatt gccgaacagc cgacagcctg
     6721 gtacgacttt gaagggttgg cccgggttac agccgcggtt gacactcctg tcatgcccca
     6781 tgaatcgctc tattcaattt acgacgccat tcagcttgac cgaatgggtg cggggaacgt
     6841 gtacggactg aagatttacc gtcccggcgg ggtcacattg gccaaaaagc tggccaccta
     6901 catggagctt cgaaacatcc cgatgttcgt ctgctcttgc gtcgaactgg cggtcagcac
     6961 agcggcttcg gcccatttcg ccgtcgcttc ctaccggaat atcaagtttg cctccgagat
     7021 gtccggccct gtaggtctgg aggacgacat tgccgaatcg gcagtccaaa taaaagacgg
     7081 catggcggca atcgttgatc gtccgggtta tggcgttgag ttggacgaaa aacggatgaa
     7141 aaaatatggg ggagaaataa ttacgattaa gtaaaggccc aatccttcgg ccgctgcaga
     7201 tagaggcgtg agcgaacccc cagccttatc tgcccgtgat gattttcttt aaagctgtag
     7261 gcttgttgct cccgggcctt tcaaaaaggc ctgattataa atacataaaa ataactcggg
     7321 gtgtatcttc agccgaatcg aactcattga acaaccgaag ttcagatttc ggagtttggg
     7381 ctctgctgat ctgaagattc tcaaataggt acgcccggaa aagtaaccct ccgggaatct
     7441 tcatatggag tcaaggttta ctgcaaggga attggccagg gggaattagc cattcttaaa
     7501 tatgctgcct tcaatgcaga taggggaaag gagagcggat gaagatatcg agtattgaac
     7561 tcgtaaatgt gaatattccc cttgctccct cggaccttcc caagccggtg ggtcgtaatt
     7621 atggttgctt catgttggtg cgcatcaaga ctgacaaagg tttggaaggc attggggatg
     7681 ggtattacgg caactcggcc ccgggagttg cggcgactat ccgtgagacc ctgactccgg
     7741 aaattatggg tcgggaggcc accgccgttg gcggactata cgaacgcatg taccgcgcgg
     7801 gtttttactc tgggcgaatc ggcatctact catatgctgt gagcgcagta gagatggcct
     7861 tgtgggattt gcttggtaaa taccacggag tccccgttta tgaactcctc ggcggaatgg
     7921 taagccgagc ggtagccccg tacccaactc tgcgggcggt aatggatgaa aaggaagaga
     7981 aaaaggtgcc ggtttatgct agtttgcaga ctttccgaac agccgaagag gtggggttga
     8041 tcgcgaaggc tgctgtggag gaaggattta aatccgttaa gcttcatcag gttgatatcc
     8101 cttctgtcag agtgacccgg gaagctgtcg gcggcggaat tgaagtcacc atggatgtga
     8161 acggggtttt taattccctg gaagcggaac gcttcgcccg tcagttggcc gagttcgaca
     8221 taggttggtt tgaagagcca atttggccgc ctgatgatta cagagctttg gcacagttac
     8281 gccggcgctc ccctgtgcca attgccggtg gggagaacga gtccacagtc tatggattcg
     8341 agcggatcct ggaaacggaa gctgtggacg ttttgcagcc cgagattctc tcggtagggg
     8401 ggctgctgga aatgtacaag gtgcttgcta tggcccaagc tcgtagccta ccggtcgccc
     8461 cgcataactt caagttcggc ccggtgctgg cggcgacgct gaacttgagc cttctttcct
     8521 ccaatgtatt taccaccgag acgccgtggt tcaaactgga ggcgaacctt tttaaaaaag
     8581 gcccccagat taggaacggc tgggcttgtc tttccgatct tcccggctta gggttcgaaa
     8641 tcgatgagga agtggtgaaa gaataccgca tagagaaatt cccccaaaaa taggggggat
     8701 caagttggta caggcacagg tgtttgagac agggccgatg gtatttatgc tttttacccg
     8761 ggcatgggca aaggggcagc gcaggactcg ggcacaaagg atgcggggga ggacctgtcc
     8821 ggctaaatga ttctcttttc ccgaagggcc cggatttctt cctttgagaa ctctagctct
     8881 ttcagaattt cctccgtgtg ctggcccaaa gtcggaggtg ggagtgatat ggagcagggg
     8941 gtccggctca attttacggg aaagccgatg aggctgattt taccggcggt taaatgttcc
     9001 gtttccaaga gcatcttctg gttgaggacc tgcggatcgt ggaagacctg agagagggtg
     9061 tagatcggcc cgcaggcaat cccggctttg ccgaggctct ccacccagtg gtcggttctt
     9121 tcggtgacta gcctggattc cagtatcctc ctaagctcct ctttatttgc caccctattg
     9181 gggttgctca tgaaacgatc atccttcaat agtcgctcga ctcccagctg ccggcagagt
     9241 tcttcccaca tccgggaggt gcccgcagcg atattgatat acccgtcgag cgtcttatat
     9301 gtgccgtacg gcgcaaggga agcgtggtcg tttccctggg gcaggggagc ctcgccggtg
     9361 ccgaagtatt tggcggcctg aaaacctaga acggcgatca gtgcttccag caaagaggtt
     9421 tcgataaatt ggcctttccc cgatttttct ctctccagca gagccccaag tatcccatac
     9481 gcggcaaaga tggcgcagag cgaatctccg atcgccacgc ctacccgggt gggtccggtc
     9541 tctgaaaatc cggtgacgct catcaaaccg gacattcctt gggcgatctg gtcgaaacca
     9601 ggagtttccg cccacggacc ggtctggccg aaacccgaga tactgcagta gatcaaacgg
     9661 ggattgatac tgctcagggc gggataatcg atcctcaatc ttgacttcac tttcggccgg
     9721 aagttttcca caagaacgtc ggctcctttg actaacttta aaaggatatc ccttcccgcc
     9781 gattcccgaa gattgagggt cattcctctc ttatttctgt tgccatacag gaaataggtt
     9841 gactcgccgc caacaaaggg gccgctcttt cgcagtaggt ccccctcgct cggctcttct
     9901 atttttatga cctcggcgcc catatcccca aggagcatgg aacaataagg gccggagatg
     9961 gcggaagtgg cgtcgatcac tctgatattt gctaagggct gaggcattga atccactcct
    10021 ttgaccatca gcgaaggcga tccacctctt cgaccgcttt cttccccggg tcgcccactt
    10081 gtttcagata gtagcccaga atgggcttgt tggccgcctc tttccacctt ttcttttcca
    10141 ttgcaggaag gtagtaaacc tcgacgcctc tcttcttcag ttcggaaagg cattcctcgg
    10201 tttctttcag agattcctgg aatccccatt cttcgatctc ctgtccgacc tcgtacattt
    10261 ctttctgaat ctccggaggc agacccttcc agatcttcag attaatcaat accgccgggg
    10321 tcaccagaat atcctggatc tcgcagtaag tcatctgttt ggctacttcg aagagcttcc
    10381 tttcgtaaac cgaaggcaag gaagtaagaa cgctgtcgat ggttccacgc tgcagggcca
    10441 tataggtttc cccggtaccc ataaacacgg gagccccgcc aaatgcctga atgagctgcg
    10501 tggtgatatc ccccaggact cggattcttt ttcccttgaa atcctcgagc ttctgaagcg
    10561 gctttttgga gatgaaggac atcagtccat agtccatcca gaaaaggagc ttcatattct
    10621 tcttttccat ttcgtcgttg atcagctgga caaaactctt ggaatgctcc accttgcgta
    10681 tgtgctcccg gctctcgaag aaataaggaa ggcccatgat ccccaagacc ggaacgagcc
    10741 cctcgagcag agccaggttg caggccccca tctcgaccga accggcaggg agcgcccggg
    10801 tcatgtcttt gtcactgaag agctgtcctg ccgggtagac ttcgatcttc aatcggccgc
    10861 cggtcttttc actcaggatc tgggagtaac gttccttggc ccgggtcata aagtgcccga
    10921 tgggcatggt cccggccatc ctcagcgtaa attcgggctt cgacgcggcg agggcggagg
    10981 gaacctcaag gcataaacag aaaaagagca ggaacaggat ggccattttc atcgcggatc
    11041 tgaaatcttt catcttctgc ctcctcatta atgattattc caggcgtatt cacccatctc
    11101 cttcaaggtc caagcacttg cggaagccaa gtggtcaaag cgggtacaga ggaaatcaga
    11161 accaggaaaa ggccgataat gaccagaaac ggaatcaccg accggctgac ttcggcggcg
    11221 ttggatttgg ccagattctg caacacaaag aggttcaaac cctcgggagg agaaatgagg
    11281 gccaactcca tgttgatgac catgatcact gcaaaccata gcccgctgaa acccaacccg
    11341 gtcacgaggg ggtagaggat gggcaacgta atcactagga tggagatcgc ttccagcggt
    11401 cctccggcga tgatcaaaaa gacgttgata gacagaatga taacccagcc ggggagttcc
    11461 attgacttaa cggcgagcag gatcttctgc gatatctgag acatgagaat cacctggccg
    11521 gtaatgttgg ctccgatgat gatgaacaaa atcattccgg agaccctggc cgtgtccagc
    11581 aggatgctcc tgatcccctg ggcgttaaac gaccggtata caagggcgca aaggacaagc
    11641 gaatagatgc tcgccactcc tcccgactcg gtaggggtaa agattcccga gtagatccct
    11701 ccaatgatga tgagaatcgt gatgagcccc cccgaagcgt cccgggtcgc ttggagaatc
    11761 tcccccaggg ttgcccggcg gtcgcgctga atttccttgt tccgccctcc gacaaaggca
    11821 acgtaagcgc agaaccccag agataggatc agacccggga tcaatccggc catgaaaagc
    11881 gaccccaatg attcgttggt catgctcgcg taaaggatca tggccacact cggcggaaag
    11941 aggattccca gggtacctcc ggcagcgagg aggccaatcg tcagagtgcg gggatacccg
    12001 cgggcgagca tttccggaag ggcaatcaaa ctaaccgcgg ccgccaccgc cacgctggag
    12061 ccggtcatag ccgcaaagag accacaaaaa atgatggaag caatgcccag cccccccggt
    12121 aaatggcgca ccaagacgtt ggcgaatctg tatattcgca cgccaacctc gcctcggttc
    12181 aggatcgtgc tcgtcaggat aaagaggggg agagcgacca tgacgtaatt gtccatcgtc
    12241 gaataggcca taaccggaag ctgcgagagc atgcgggggc cgccgaagag aaaatagaaa
    12301 ccaaacatcc cgaaaagccc cagggcgatc gagacgggaa tcccgcttaa aaggagaacg
    12361 aaaaaaagaa ccagtaagcc gaggttcatg gatacgccga gaagaaaaag gctgaaaacg
    12421 aggagcacgg cgaacccgcc gcagatagcc atttgcgatc cgccggaaga ttcagcggca
    12481 tcctttcctt taccatccgc taaatggctg aacagatcgc ggatgtactt gacaaaggtt
    12541 acgcccagaa gggtactgcc gacgggtatg aacacctggg ggataaacat gggaacttta
    12601 aggattgtgg gggaaacctc tccgaggcga aaagagttca gaaccaattt catgccttcc
    12661 cacgccatga ccccgcaata aacaatggcg agaagggagg tgatgatctc gaggctgatc
    12721 gttgttctgg gtggaagccg attgattaca aaatcgactc gtacatgggc tttgccttta
    12781 aggacgaagg caagggaaag aaagatcccg gccagggaaa aatagatgct gatttccatt
    12841 acccaggtcg tgggggcgtt gaagaaataa cgcatcagga cttcatacgt gaccatcccg
    12901 gccacacaca ccaggagggc ccccccgagc agccctatcg cgatgaccat ccggttgaaa
    12961 aatgattcgg gacttgcggt actctgcatc ctcaaatttc cttcgaggtc actggtcaac
    13021 agcgttccct cgctctgagg cacgtagtct gccccctttt ataattcctt ataagaataa
    13081 aagtcaagat gagttaatcg taacgaggga aatggaggag atgcgcccgg gaaagtctgg
    13141 aggaacggcc caggaattac tttattcaag ccagggtcag accgtcgccg ataggagtta
    13201 ggctaacgtc aacccgctcg tctccctcgc ccttttcata cagttcgctg agggcccggc
    13261 ggcacactgg gcaaaagagg catcgcctcg cccttcatct ctcttggggg gagatcattt
    13321 tccttttttc cctctcccct ctgtagaggt ctcccggccg gcggcaatcc ggtctggatt
    13381 tgaggcgagt tgaacctttc cgacctgatc cacccatagc tgataggtaa gctgcatatg
    13441 gccaaggtgg gcggcgtagt gcgagatggc gtgcaagata caccggcgga cggttcctga
    13501 gccggtgagc cactttctcg actcctccag ttgggcaggg gtcagagggg aaaggatctc
    13561 ctccgccatc ccagttcccg ccgaaagcct tctcaggagt tcggctactt ccaccccttt
    13621 ggttctaaat tcctcctccc ggtcgcgctg gatcggccgc ccgccgatta cgtgggcgat
    13681 caaaaaattt tcggagccgg ccagatgggt caccaaagca gcgggcgagt tggtggccag
    13741 atcgcccgtc ccctcaaggg gtcgccagtc gagggattcc gctgagaatt taacgagcaa
    13801 atcccggacc tggccgcgga gatgctccaa gaccaacaag taattttcca cttcgttcaa
    13861 cattttctcc ctcctcggga ttttaggatc cttttattcc cgccccattt taccaaaacc
    13921 gaaaaaaaag gaaaaatctc ctcgcccgtt ttcgcccaga aggtccttgc ccccgggcct
    13981 ggcaccaaat cgagggccac ctttaggtgg cgcccttcaa tccgcccgcg gtcgggtctc
    14041 atcgactttg aacggttcac tttttttcat acctccggct ctgccggagg tcatgattcg
    14101 agaaggcagt gaattaaggc ccaaagggaa tttctgaatc taattggagt cttcagatta
    14161 ggtctttcgg gaacccccgc cttttcattt tactcctcag aaggctaaag gttaggctgt
    14221 ccgggttact cgcaatggag ggagctaacc aatactctta tggcaatcgg tagtcaaggc
    14281 aaaaattcaa ggcaattcgg cggtttaaaa atgataggga gacatgttgc cccttcaccg
    14341 tttctcaggt taaaatcgcc gccaataaca ccgccataat tccactcaga aatattccat
    14401 caaaagttcc tgcgcccccg atggaggcga cgggggcacc caggttcttg attttagaga
    14461 cattcaggat atccgccccg atcagagttc cgagggtgcc gccgatgtaa gccaggactg
    14521 gagcatagtc gtaggagaaa atcaccgaaa agaccgcagc gataatggga gggatgaagg
    14581 ccggcatggc aatgcccact cccttgatgg gcttggccag tcgatgggtt acaaacacca
    14641 tgatcgccgt caccgccagg cccttcaccc agagtccggt cttaaaaaat aaataaaaag
    14701 aaagcaggca gggaatgact gcacctccaa ggtttaccgc caggaccgtt tccggttttt
    14761 ctgccggggg aatgcggtag cgaatgccaa aaaagctaac cgatctttca ttaatcatcg
    14821 tttcttgggg aaatttcttg atgggtatat tgatgaaact accgatcaat ataaggaaaa
    14881 gagaggcgaa aatgtgtcgg gggggaattc caatcttcgc gaagaccagg gcgattacat
    14941 tcacctcgac catgagaaaa aagaagagga cgatgaagag aaagaagatc atcagcagga
    15001 aagtcagggg attaaaaaac ataaagccct cccttttgat cgtccggttt aaaaatcttc
    15061 agtctttgcc attctcaaat gtcttttttt atttcccacc gaggggaaag ggctatgctt
    15121 ttgggtttcg ttggtcaaag aggagaacga cggctagaaa atactcctgc gggactgggg
    15181 tagtcaaggc aaaatttatc cacgtatgaa agccccgaaa ggaaaaagat gtgccttgat
    15241 aatgactcgg gaatttgcat ccaatattgc ataaatcttt ccaatttcac ataggtgtat
    15301 tgggtcaatt tttgaggcaa attctatccg atccatccga aaacaccaaa aagccgtaaa
    15361 tcggtggtta ataaaaaatc ctctcccttt gcagcagcct ggtcaatggc cctgttcaag
    15421 cgacttaccc cggcttcata ttcccccttt gaaaggatgg tcagctgtga ggtaaaccca
    15481 cgttcgaaca agccctcgga ggcagcgagt cgcgcgggta gcgtcgcggt aatgtgctcc
    15541 acctcctcaa cctcgcagct aataaacccg gatcgcccca tggcagcttt aatttctgcc
    15601 gttgggagat agcgttcttt gtcgagcttc actgtttctg gaaagtattc gtaaatccac
    15661 cagttgtctt gcccgctatg gggatccaac ccgatcatca tcagcccgcc tgaaggcctg
    15721 aggactcgcc gggcttcgaa taaaaaagcg gatttatcgg caaaatggtg gaaggcgtta
    15781 atgcaaaaga cccggtcgaa acaaccatta ggaaagggga gattttccgc tcgtcctctc
    15841 acaagcgccc gatcggaaga aacttctcgg gcacgcagga gcatatttgt ggagggatca
    15901 actcctacgg gaatcctccc gttcttcgac aaaaggtcaa gccaatgccc cgttccgcac
    15961 cccacttcca gcgggcctgc cgattttggg gagctgacaa agcgcaaaag agcttgttcg
//
LOCUS       DASPRR010000407         7698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_749868, whole genome shotgun sequence.
ACCESSION   DASPRR010000407 DASPRR010000000
VERSION     DASPRR010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749868"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VEL68_14950"
     CDS             <1..1402
                     /locus_tag="VEL68_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ37318.1"
                     /translation="VGVLPGLGPTAAMSLLLPATFGISPVGSMIMLAGIYYGAQYGGS
                     TTSILVNIPGEAASVVTCLDGYQMARQGRAGAALGISAFGSFIAGTLALLGLTFLAPV
                     LVEAALTFGPPEYFTLMCLGIVVLTFLSHGSMLKALIMAVLGLWVGTIGIDSITGVQR
                     YTFEKPELMEGVGLVQIAMGMFGVSEILSNIEARVEISVFKTKVRGLFPTREDWRKSV
                     GAILRGAGIGFFLGILPGGGAVISSFASYAIEKRVSKYPEKFGTGVIEGVAAPESANN
                     AAAQGAFIPMLTLGIPSNIVMALLLAALMIHGITPGPLLMEKHPGLFWGFITSMYIGN
                     VMLLVLNLPLIPLWVKFLRVPYPILFPFIFLFCLVGVYTINNTVFDIYLMILFGVFGY
                     VARKFNYEPAPFILAVVLGPMMEKALRQSLIMSKGEFSIFLSRPISAVILSVAIFLLF
                     FPLVLQGIKHYRKKAA"
     gene            1525..2499
                     /locus_tag="VEL68_14955"
     CDS             1525..2499
                     /locus_tag="VEL68_14955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37319.1"
                     /translation="MERKIFAVTVAMMLGLYLLPPQAVGQDYPKREIELIAAFAPGSN
                     TDNFARLAAKHAEKFVGKPIVVVNKPGGGGSLGFATLAKAKPDGYTIGLLSNAVIGHP
                     YLLKGVTFHYKKNFRLIAQIDFSAQGLYAKKGSPYDVSLKELVKKAKEKQDTIKVGIG
                     GTWTAQDFARAIFEEEAGVKFVKVPFPGAGEAVPALLGGHVDLEFGPASEWAHLLKGG
                     KVSVLAVSTEQRDPRFPEIPTFKEQGFDVVLSTIHWIAAPAGTPDSIVNVLAEAFKKA
                     VSERGFKETADNLGATAAWESPEGSIKSMERLDQLYQRIVKKYDLKPQ"
     gene            3085..4317
                     /locus_tag="VEL68_14960"
     CDS             3085..4317
                     /locus_tag="VEL68_14960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ37320.1"
                     /translation="MTREERMIVALTGCSHALSHAYLLTFPAVLLLLKEEFSIGYFGL
                     GVVSNIMTFAYGLGSLPGGMIYNRFGPKKLYLLCFLGSAAASLLVALSPNFLLFTAGL
                     AILGALGSIYHPLVNVLITSKVKQYGVALGIHGGAGNIGVAVSPVISGLIASRWGWRW
                     AYVGFALPGVLLSLWSLTVDMSGSRSEEEKTSLAEAPGLPAAGNRVGLWGYFTLPLAL
                     LYLMNCLQSFGFNGSVTFLPSFMAQFSSFHLFSWDSVAMGGMLSGLALLTGVFGQYTG
                     GVLAQRPNPERNLVFLTASGFPFIALMSFLRDLPLLFVALVFFFFNFSLQPMMNVMLA
                     QNASPRMRGTAFGIYFFAAFGLGSLAASFSGYIAETFGLRWVFLGLSGGVLLAICSST
                     VFWNVAKRPNIGGGGTKE"
     gene            4368..4748
                     /locus_tag="VEL68_14965"
     CDS             4368..4748
                     /locus_tag="VEL68_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ37321.1"
                     /translation="MINGLDHVHIMCRDLEKSVQYFTDLFGGKVETRTELRGFPMVRM
                     SVQGIHFNLHKTDSKAAPFETGKATPGLDHIGFLVKGLEKVVEDLKKKGGKFSVDFTH
                     GAGGVKFAFIDGPDGIRIELIERP"
     gene            4932..6509
                     /locus_tag="VEL68_14970"
     CDS             4932..6509
                     /locus_tag="VEL68_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37322.1"
                     /translation="MKKRNHLGFILIVLLAVLWIPQSGLAQKPLVIGFEGDSATMDPH
                     ARSETTTTTMQRHVYEQLVNYDENLKIIPELAESWKLVNDNTWEFKLRKGIKFHNGEP
                     FNAAAVKFSLERCKTHPKSQYKQSVPDYREIQAVDEHTLRLVTKSPTPEALVMLEQVS
                     MMPPKWTEEWDKKDYSYLTRNMIGTGPYKFVEWVKDDHVSLVANKDWWGPKLDFEKVT
                     LRPIPEAATRVAALISGEIDACWGVSIPDIPRVEKTKNTYVSRVPSIRTIYVMFDVHS
                     DKGGPAPGAQPGIPTGKPNPFKDFRVRKAIAHAINVDDIIKFVMEGSAYSASQIASPY
                     AVNFDKNIQRPKYDPELAKKLLAEAGYANGFEFNFDTPNDRYVNDQQVAEAIAAQLTK
                     IGLRPKVIATPKAVFFPKMDRYESPFFLLGWGTITWQGTFDVFFREKRGAYGHINRGR
                     FKDPEVEKMIDIANSVMDDAKREKLRNEVAEKIYATYYVIPLYYQENVFGFTRRIKDG
                     KARVDERLFAHLLKLAK"
     gene            6611..7531
                     /locus_tag="VEL68_14975"
     CDS             6611..7531
                     /locus_tag="VEL68_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37323.1"
                     /translation="MGIYLLRRLYQAILVLLVVSGIVFVILHISGDPLALLMPQDAPP
                     GDIEEMRRSLGLDQPLIVQYALFLRNAAQGNLGVSYHHGQSALKLVLERLPASLELVV
                     ATIVISLLLAVPIGVLAASRRGSAWDRAALLGSLVGISAPPFWIGIAFIFVFCVELQW
                     LPSSGRGTWVHLILPAGSLALYRLALFIRLIRAGMLDVLTMDYIRTARSKGVSEQKVV
                     YKHALKNTLIPFVTIAGMQMGSLLAGAIVTEKVFAWPGMGRLFLEAIGVMDFPVIIAW
                     TLVTATIFLSVNLAVDIIYVWLDPRIRHEK"
BASE COUNT         1559 a         2200 c         2231 g         1708 t
ORIGIN      
        1 ggtgggggtt ctgccaggat tgggtcccac cgcggccatg tctttgcttc ttccggcgac
       61 cttcggcatt tcacccgtgg gatcgatgat catgctggcg ggaatttact acggcgccca
      121 atacggcggg tccaccactt ccattctggt caatatcccc ggcgaagccg cctctgtggt
      181 gacctgcctg gatggatacc aaatggcccg tcagggaagg gccggagcgg cgctgggaat
      241 ctcagccttc ggctctttta tcgccgggac cctcgccctc ctggggctca ccttcctcgc
      301 tcctgtcctg gtggaggcag ctctcacctt cggtccgcct gagtatttca ccctcatgtg
      361 cctgggaatc gtggtcctga cctttctgag ccatgggtcc atgctgaaag ccctgatcat
      421 ggccgtgctg ggcctttggg tcggcactat cggaattgat tccataaccg gagtgcagcg
      481 gtacaccttt gaaaaacccg aactcatgga gggagtaggc ctggtacaga tcgccatggg
      541 aatgttcggg gtctctgaaa ttctttcgaa tatcgaagca agggtggaga tcagcgtatt
      601 caaaacaaag gtcagagggc tattcccaac ccgggaggac tggaggaaga gcgtgggggc
      661 catcctccgc ggagccggaa tcgggttctt tctgggaatc cttcccggcg gaggagcggt
      721 catttcctcc tttgcctcct acgccatcga gaagcgggtc tccaagtatc cggagaaatt
      781 cggcaccggc gtgatcgaag gggtggctgc cccggaatca gccaacaatg ccgcagccca
      841 gggagctttc atccccatgc tgaccctcgg gatcccatcg aacattgtca tggccctgct
      901 cctggcggcg ctgatgattc acggaatcac tcctgggccg cttctcatgg aaaagcaccc
      961 cggccttttc tggggcttca tcaccagcat gtacatcgga aatgtgatgc tgctggtcct
     1021 gaaccttccc ctcattcccc tttgggtgaa gtttctccga gttccttacc ccattctctt
     1081 cccctttatt tttctcttct gtctggtggg ggtgtacacg atcaacaaca cggtctttga
     1141 catttatctc atgattctct tcggggtctt tgggtacgtc gcgagaaagt ttaactatga
     1201 gccggccccc tttattctcg cagtggtttt ggggccgatg atggaaaagg ccctgaggca
     1261 atcgctcatc atgtccaagg gcgaattctc catttttctc agccggccga tttcagcagt
     1321 tatcctgtcg gttgcgattt tccttttgtt ctttcccctg gtcctccagg ggataaagca
     1381 ttatcgaaaa aaggcggctt gatcgtttta gggcgaacag gctgtagggg cgatttacga
     1441 atctccacta ggacgatctc ctttcttcga ggaagtgacg atgagggcgg gcctaagctc
     1501 atttgcttta aaaggaggga aaaaatggaa cggaagatct ttgcagtcac cgtggcgatg
     1561 atgctaggcc tgtatctcct gcccccgcag gccgtgggtc aggactatcc caagcgggag
     1621 atcgagctga ttgcagcctt tgctccgggg agcaacaccg ataattttgc ccggctggcg
     1681 gccaagcatg cggaaaagtt cgtagggaag cccatcgtgg tggtcaataa accgggggga
     1741 gggggatccc ttggttttgc cacgctggcc aaggcgaaac ccgacgggta caccatcggg
     1801 ctgctctcca acgcggtgat cgggcatcct taccttttga agggagtaac cttccattac
     1861 aagaaaaatt tccgcctcat tgcccagatc gacttcagcg cccaggggct gtatgccaag
     1921 aaggggagcc cttacgatgt ttcgctgaaa gagttggtca agaaagccaa agaaaagcag
     1981 gatacgatca aggtaggaat cgggggcacc tggacggccc aggactttgc ccgggccatc
     2041 ttcgaagaag aggctggggt caaattcgtg aaagtccctt ttcccggcgc gggagaagcg
     2101 gtcccggctc ttctaggcgg ccatgtggat ttggaatttg gcccggcctc ggaatgggcc
     2161 catctcctca aagggggcaa agtcagtgtt ctagccgtga gcacggagca gagagaccct
     2221 cgcttcccgg aaattcccac tttcaaagaa caggggtttg acgtggtatt gtccactatt
     2281 cattggatcg cggcccccgc gggaacgccg gattccatcg tgaatgtcct ggctgaggct
     2341 ttcaagaagg cggtcagtga gcggggtttc aaggaaacag ccgataacct gggcgctacc
     2401 gccgcctggg aaagccccga ggggtccata aagtccatgg aaagactcga ccaactctac
     2461 cagaggatcg tgaagaaata tgacctgaaa ccacaatgag gaaaggggtg ggaatgcctg
     2521 gaattccttc ggtgaatacc ttgcccaccc ccagtttttc gtggttgttg gggttcaggg
     2581 tgaaggaatt tggatcatca ggctcggcgg gtgcttatcc ttccatgaaa ctccccccca
     2641 tgttccgcag ggagaggcct gagaggagat tcctccaggc agaaaagaga gccaaagcat
     2701 ttttctgttt caatcccccc gggacaggcc cgatccaatg gaagaaccac cgcctcagga
     2761 tgatgatcca aatttctcca ccctgaaccc catgatatcc cccctccttt ccagagatct
     2821 tattttcgaa ggactctttt ttaagaactg ggggtgcgca agcggtgaat atctcttgtc
     2881 ggaagcaaga aggtcatgtt aaaagagagt tgagctgaaa tggaggcaag ggagaaaggg
     2941 ttgaatggca ggcccttgaa ataggattct ccccttaaaa agacagatac cgagcaaacc
     3001 cgaaggtgat cacaagagac tgccccctca tcatgagaag gtatgaatga acactccgca
     3061 ctcgagacaa aaaactcatc cgtgatgact cgcgaagaac ggatgatcgt tgccctcacc
     3121 ggctgctctc acgccctgag tcacgcttac ctgttgactt tcccagctgt gctcctgctc
     3181 ctgaaggagg aattctctat cggctacttc gggctggggg tcgtcagcaa catcatgacc
     3241 ttcgcctatg ggctgggctc tcttccgggg gggatgatct acaaccgctt cggccccaag
     3301 aaactatacc tcttatgctt cctggggtca gcggccgctt cgctgctggt cgccctgtcg
     3361 cccaactttc tgctcttcac cgcggggctg gcgattctcg gggcgctggg aagcatctat
     3421 caccctctgg tcaatgtatt gattacctcc aaagtaaagc agtacggagt ggccctggga
     3481 attcacgggg gggcggggaa catcggcgtg gccgtctctc cggtgatctc cggcctgatc
     3541 gcctcccgat gggggtggcg ttgggcctat gtgggctttg cccttcccgg ggttttgctt
     3601 tccctctggt cccttacggt ggatatgtcg ggctcccgga gcgaagaaga aaagacatcc
     3661 ttggccgaag ccccgggtct gcccgcggcg gggaaccggg tcggtctctg gggctacttt
     3721 acccttccgc tggccctcct gtacctcatg aattgcctgc agagtttcgg cttcaacggc
     3781 tcggtcacgt ttcttcccag cttcatggct cagttttcct cttttcatct attctcctgg
     3841 gacagcgtgg ccatgggggg gatgctgtct ggcctggccc tcctcacggg ggtattcggg
     3901 caatacaccg ggggggtgct ggcacagagg cccaacccgg aacggaacct ggtgtttctg
     3961 acggcgtcgg ggtttccctt tatcgccctc atgtcctttt tgcgcgacct ccccctcctg
     4021 ttcgtagccc tggtcttctt tttcttcaat ttttcgctcc agccgatgat gaacgtgatg
     4081 ctggcccaga atgcctctcc gcggatgcgc gggacggcct tcggaattta ctttttcgcc
     4141 gccttcggtc tcggctccct ggcggccagc ttctcggggt acatcgccga gaccttcgga
     4201 ctccgctggg ttttcctggg gttgagcggc ggcgtcttgc tggccatctg ttcttccact
     4261 gttttctgga acgtcgcgaa gcgcccaaat atcggtggtg gggggaccaa ggaataagga
     4321 tcgcgatttg cgccttgtgg aatctatctg aagaaaggag aagcgagatg atcaacggat
     4381 tagatcatgt gcacatcatg tgcagggacc tggagaagtc ggttcaatat tttacggacc
     4441 ttttcggcgg aaaggtggaa acccgcacgg agttgcgcgg atttccaatg gttcgcatgt
     4501 cggtccaggg gatccacttc aacctgcaca agacggactc aaaggccgca ccgtttgaaa
     4561 ccgggaaggc gacccccggg ctggatcaca tcggttttct cgtgaaaggc ttggaaaagg
     4621 tggtcgagga cctgaagaag aaaggcggga aattcagcgt ggacttcacc catggtgccg
     4681 gcggggtcaa attcgccttc atcgacggcc cggacggcat ccggatcgag ctcatcgaga
     4741 ggccgtgaaa tggggggatt aaatccttga cttcgacccc ctcttgaata tactgaattt
     4801 tgttcggcct ggctgatttc tgaaaagcgt gtcctctctc caaggttgga ggagaaagag
     4861 gggaggggga cccatgctct cttggggtag cggtgttttg agcaaccgtt cgtgtcttga
     4921 aggaggggaa gatgaaaaag cgcaaccacc tggggttcat cctcatcgtt ttactggcgg
     4981 tcctctggat tcctcaatcc ggcctggccc agaagcccct ggtcatcggg ttcgaaggag
     5041 actcggccac gatggacccc catgcccgga gcgagacgac caccaccacg atgcagcggc
     5101 acgtgtatga acagctggtc aattacgacg aaaacctgaa gatcatcccc gagctggcgg
     5161 agagctggaa gctggtcaat gacaatacct gggaattcaa actgcgcaag gggatcaaat
     5221 tccacaacgg ggaaccgttc aacgccgcgg cggtgaaatt ctccctggag cgctgcaaga
     5281 cccatccgaa gagccagtac aagcagtcgg tgcccgatta ccgcgagatc caagccgtcg
     5341 atgagcatac cttgcgcctg gtcaccaagt cgcccacgcc cgaggcgctg gtcatgttgg
     5401 agcaggtcag catgatgccc cccaaatgga ccgaggagtg ggacaagaaa gactactcct
     5461 acctgacccg caacatgatc gggacggggc cctacaaatt cgtggaatgg gtaaaggacg
     5521 accatgtctc cctggtggcc aacaaggact ggtggggccc gaagttggat tttgaaaagg
     5581 ttaccctgcg gcccatcccg gaggcggcga cgcgggtggc cgccctcatc agcggggaga
     5641 tcgacgcctg ctggggagtc tcaatcccgg acatcccccg ggtggagaag accaagaaca
     5701 cctatgtcag ccgggttccc agcatccgga ccatctacgt catgtttgac gtgcactcgg
     5761 acaagggcgg gccggccccc ggcgcccagc ccggaattcc cacgggcaaa cccaacccgt
     5821 tcaaggattt ccgggtccgc aaggccattg cccatgccat caacgtggac gacatcatca
     5881 agttcgtcat ggaggggagc gcctactcgg cttcccagat cgccagcccc tacgccgtga
     5941 atttcgacaa gaacatccag cggcccaagt acgacccgga gctggccaag aagctgctcg
     6001 ccgaggcggg ctacgcgaat gggttcgagt tcaactttga cacgcccaac gaccggtacg
     6061 tgaatgacca gcaggtggcc gaggctatcg ccgcccagct taccaagata ggtctccgcc
     6121 cgaaagtgat cgccactccc aaggcggttt tcttccccaa gatggaccgg tatgaatctc
     6181 ccttcttcct cctgggctgg gggaccatca cctggcaggg aacctttgac gtctttttcc
     6241 gggaaaagag gggggcctac gggcacatca accgagggcg gttcaaggat cccgaagtgg
     6301 agaagatgat cgacatcgcc aacagcgtta tggatgacgc caagcgggaa aaattgcgca
     6361 acgaggtcgc cgaaaagatt tacgccacct attacgtcat cccgctttac taccaggaga
     6421 atgtcttcgg attcacccgg cggatcaagg acgggaaggc ccgggtggat gagaggctct
     6481 ttgcccacct gctgaagttg gcgaaataac cccgcggtcc tctttcacaa ttcgccgagt
     6541 atcaacgggt aggggcaacc acgccaaagg cctggtgaat tgcccctact ttttctctgg
     6601 ttcggaaggt atgggaattt acctgctgcg gcgcctatat caggcgatcc tggtcctgtt
     6661 ggtcgtatcc gggattgtgt tcgtaatcct ccacatctcc ggcgaccctc tggccctgct
     6721 catgccgcag gacgccccgc ccggggacat tgaagagatg cgccggtcgc tggggctgga
     6781 ccagccgctg atcgtccaat acgccctctt tctcaggaac gccgctcagg gaaacctggg
     6841 ggtcagctat caccacggcc aatcggcgct caaactggtg ctggaacgcc tgccggcgtc
     6901 gctggaactg gtggtggcta ccatcgtgat cagcctcctc ctggccgtcc caatcggcgt
     6961 cctcgccgct tcacggcgcg ggtcggcatg ggaccgggcg gccctgttgg ggtccctcgt
     7021 cgggatctcc gctccccctt tctggatcgg gatcgccttc atcttcgtat tctgcgtgga
     7081 actgcagtgg ctcccttcgt ccgggcgggg gacctgggtc catctgatcc tccccgcggg
     7141 ctcgcttgcc ctttaccgcc tagccctctt catacgcctt atccgggctg gcatgttgga
     7201 tgttttgacc atggactata tccgtacggc ccgatccaag ggggtgagcg agcagaaggt
     7261 ggtctacaag catgccctca agaatacgct cattcccttt gtcactatcg ccggaatgca
     7321 gatgggctcg ctgcttgccg gggccatcgt cacggaaaag gtcttcgcct ggcccggcat
     7381 ggggcggctt ttcctggaag cgatcggggt catggatttt ccggtcatca tcgcctggac
     7441 actggtgacg gccaccatat ttttatccgt aaacctggcg gtggacatta tttatgtctg
     7501 gctggacccg cggattcggc atgaaaaatg aatcctgaga cgacggcccg aacacttccc
     7561 agtcccctcc ccgaggaacc ctacagcccg ccccgggcac agttctgggg gtacgagtat
     7621 gtcccggcag agcggcagct tctccactcc ttcctgtcca accggctggt ccttgtcgcc
     7681 ctgtggatgc tggcgctc
//
LOCUS       DASPRR010000408         5414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_100821, whole genome shotgun sequence.
ACCESSION   DASPRR010000408 DASPRR010000000
VERSION     DASPRR010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100821"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VEL68_14980"
     CDS             <1..301
                     /locus_tag="VEL68_14980"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXZ37324.1"
                     /translation="GGHVRIEVLVSRFRGKLYDWVRLIGLILTFIFMIELDRAAYKMI
                     VYAVQINLRSSTWLMFPLFWPQLTVFIGFILLTLLLVIDIFRTGVKIRAGRKAEA"
     gene            306..1613
                     /locus_tag="VEL68_14985"
     CDS             306..1613
                     /locus_tag="VEL68_14985"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ37325.1"
                     /translation="MSLELQSLILLGGMCFLLAFGIEIAPAMGIVAGLGLIFFVNQPM
                     DYFARAAFDIMNSFTFTAIPLFVFMGTIYATTGIVRGLFVGAEKIFGNVPGSLASAMI
                     VANAIFGAISGSSLAAAATFGRICFPEMERAGYDPKMSLGAIAISGTLSVLIPPSLIM
                     IVYGGWQDVSVARLFAGGVVPGIILTIFFVITVTILVLRNPSLAPKTATRQYSVKEKM
                     TALWDIFPFLVVITMVLGTTFSGIMTPTESASLGAFLSIILALLFRQMTYPALKESML
                     SAVNVSAMIAFVLFAARMLGQVFQYIGLTEATSTFMMNLDLGKYAMLTIICILYLILG
                     CFFDSLSMLVLTLPFVAPIIKNLGFDFIWFGVLYVVLAEIGLVTPPFGLNLFVLKGVV
                     PKYEITYIFAGTVPFLIPTCILLVLMILFPDLVLWYPNLLYAK"
     gene            complement(1802..2773)
                     /locus_tag="VEL68_14990"
     CDS             complement(1802..2773)
                     /locus_tag="VEL68_14990"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HXZ37326.1"
                     /translation="MGKERPKPSSHPPVLPNVSPQEMMSLLELQKNKGEELLKKNLIQ
                     LGDVRSWNLSTQEILTKAFGSNSGYIDSIIHRGDHKPYPVYEPESSLEKQRRKNLQMA
                     LTQLKGCIEHLQNYHPEANDSPPSVQKEIQEPEEKAKITVDLDWVEKKKEPTPEEKKD
                     NQPEVIKKEKAMEKTSIPKVFVITGKDEKKKAAVASFLASLGVEPVIPQEDNGHKTNL
                     VEKLGKNAEVAFAIILLVGDEVGYPKEKPEESRPRANQKVIFELGFLMGRLPQNLVCA
                     LYEEGLDLPAEYQGNVFIPFDGGGIWKLLTARAMKMAQVDIDMNKAV"
     gene            complement(2956..3987)
                     /locus_tag="VEL68_14995"
     CDS             complement(2956..3987)
                     /locus_tag="VEL68_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ37327.1"
                     /translation="MEDYPMAMVTAPGKVDFVKRTLPPIGDHDVLLRVKASSICGGDI
                     HLYRGKHPLASLPMAIGHEISGEIIQAGHAVTKVTVGDRVAVEPVITCRTCPFCLRGE
                     YHLCENIRYQYSAGQGGFTPYFVASQDWVHRLPDSISYEEGALLEPLAVAVHAARKGE
                     VGLGHSAAIFGAGGIGLFLLQVVRAGGCGSVFISDLLEHRLETALKMGATAAIHAAKE
                     DPVRRILEETGGLGVDRSFEAVGVEKTLYQSAQCLKKGGICALIGLFEAPHEVHFPVN
                     LFAQKEIQIRGCRGYCWDFQIALDLVKKGVIRLRPLISHQFPLADLSRAFETLLDPKG
                     QANKVIIQA"
     gene            complement(4246..>5414)
                     /locus_tag="VEL68_15000"
     CDS             complement(4246..>5414)
                     /locus_tag="VEL68_15000"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ37328.1"
                     /translation="GKELKEVRKMMGELFSLIGPAPKLIPPKSRVLIKPNLTAEESLW
                     EKGILTGPVFMQALVEEVQKARPAEVIIAEAIAIGLNTKKAFAANGYEEMARTTGARL
                     LDLYDGEFEEIKTPSGGLLESVRVSREVLRADFFINAPVLKTHFASTLTAAMKNLKGT
                     TTYDEKKRFHYLGLNKAFTELNAVLKPHLNVVDGLIAMEGDGPVAGSPVGLNILMAGT
                     DAVAVDTIAARVMGIDPTEVLSLCLAQGMGLGVWDEKDITVVGRSIEEVRRPFVRACA
                     PLELDAEKVHFLNGQACNACPNGLRIAMGRLKAAGISLEKLPRVEISVGSEARLSGSA
                     DYIQLPIGNCQKQYKHLPNYVPGCPPPAFLMGDQVREIMGLPRRFGPKKNFIME"
BASE COUNT         1085 a         1507 c         1439 g         1383 t
ORIGIN      
        1 aggggggcat gttcgaatcg aagtcctggt aagccgcttt cgtggcaagc tctacgactg
       61 ggtccggctg atcgggttga tcctgacctt tattttcatg attgaattgg accgggcggc
      121 ctacaagatg atcgtgtatg cggtgcagat caatttacgg tcgtcgacct ggcttatgtt
      181 tcctctgttc tggccccagt tgacggtatt catcggtttt atcctgctca ccctgctgct
      241 ggtgattgat attttccgga ccggggtcaa gattcgggcg ggcagaaagg cggaggcctg
      301 aaaaagtgag tctggaactg caatccctga ttcttctggg aggtatgtgt tttcttttgg
      361 cctttggcat cgagattgcg ccggccatgg gaatcgttgc cgggctcggc ctgatcttct
      421 tcgtgaatca accgatggac tattttgccc gggccgcttt tgacatcatg aactccttca
      481 cttttacggc catcccgctc tttgttttca tggggaccat ctatgccacc accgggatcg
      541 tgagagggct tttcgtggga gccgaaaaaa tattcgggaa cgttcccgga agcctggcct
      601 cggccatgat cgtggccaac gccatttttg gtgccatctc cggttccagc ttggcggcgg
      661 ccgccacctt cgggcgcatt tgctttcccg agatggaaag ggcgggctac gatcccaaga
      721 tgtccctcgg ggccatcgcc atttccggga cgctgagcgt cctcattcct cccagtctca
      781 ttatgatcgt ctatgggggc tggcaggatg tatccgtggc ccggctcttt gccggagggg
      841 tggtgccagg catcatcctg accatatttt tcgtgatcac cgtaaccatt ctggttttgc
      901 gcaatcccag cctggccccc aaaaccgcaa cccgtcaata ttcggtaaag gaaaaaatga
      961 ccgccctgtg ggacattttt cccttcctgg tggtcatcac catggttttg ggcacgacct
     1021 tctccgggat catgaccccc acggagtcgg cgtccctggg ggccttcctg agcatcattc
     1081 ttgccctgct cttccgccag atgacttacc cggccctcaa ggagagcatg ctctcggcgg
     1141 tcaacgtgag cgccatgatc gcctttgtcc tgttcgccgc ccggatgctg gggcaggtct
     1201 ttcagtacat cggcctgacc gaagcgacct cgaccttcat gatgaacctg gacctgggaa
     1261 aatatgccat gctgacgatc atctgcatct tgtatctaat cctgggatgc ttctttgact
     1321 ctctgtccat gctggtgctg accctcccct ttgtggctcc catcatcaag aatctgggat
     1381 tcgatttcat ctggttcggg gttctctacg tggtgctggc ggaaatcggt ctggtcactc
     1441 ctcccttcgg cctgaacctc tttgtgctga aaggcgttgt gccgaaatat gaaatcacat
     1501 acatcttcgc gggcaccgtt cccttcctga ttcccacctg tattcttctg gtgctgatga
     1561 tccttttccc cgacctggtt ctatggtatc ccaacctgct ttacgcgaag taatcgctcc
     1621 gctctcagct ctcggtaatt cactgcaatg gtttgagaag atttctagga ttccgtgtgg
     1681 gtcccggaaa gagaccttgt ccaggagacc ctttgcttgg ctgaagaaat caggggaggc
     1741 ttaaaaaata ccgctggagg agagcattag gttcggaaaa ctggcataaa tctgtgatat
     1801 cttaaactgc tttgttcata tcaatgtcca cctgagccat cttcatggcc cgagcggtca
     1861 acagcttcca tattcctccg ccatcaaagg ggatgaagac atttccctgg tactcggcag
     1921 gcaggtccaa tccttcttca tacagggcgc agaccagatt ttggggcaac cgacccatta
     1981 aaaatccaag ctcaaaaatc accttctgat tggcccgggg cctggattcc tccggctttt
     2041 ctttgggata accgacttca tctccgacga gaaggatgat ggcaaaagcc acttcggcgt
     2101 ttttcccaag tttttcaacc agattggtct tatggccatt atcttcctgg gggatgaccg
     2161 gttctacacc caggctggcc aagaaagagg ccaccgccgc ctttttcttt tcatccttgc
     2221 cggtaatgac gaaaactttg ggaatgctgg ttttttccat agccttttcc tttttgatga
     2281 cttcgggttg gttatctttt ttttcctctg gagtcggctc ttttttcttt tccacccaat
     2341 ccagatcgac ggtgatctta gctttctcct ccggctcctg gatttccttc tgaaccgatg
     2401 ggggggaatc gtttgcctcc ggatggtaat tttgcaggtg ctctatgcag cctttgagct
     2461 gggtcaaagc catctgaaga ttcttgcggc gctgcttctc caggctcgat tccggttcgt
     2521 ataccggata aggtttgtga tctccccgat ggataatgga gtcgatgtat ccagaattgg
     2581 agccgaaagc cttggtgaga atctcttgag tggagaggtt ccaggatcga acatccccca
     2641 gctggatgag gttttttttc agcagctcct ctcccttatt cttctgaagc tcgaggaggg
     2701 acatcatctc ctgcggggag acattcggca acaccggagg gtgagaagat ggtttgggtc
     2761 gctctttacc catgaatgat tccgtttgga ttaaaattta ttcagcagga gagccctact
     2821 tttttcttga atccgcgcca ggcggagtga ctggaactgc cctggataaa gccctatgtt
     2881 ttttattagt tatcgtcaat cgggaggaaa accttaaagg aacgggcaag gaggaaaaaa
     2941 aggtcaccct gagaatcagg cctggattat gactttgttt gcttgacctt tagggtcaag
     3001 aagggtttcg aaagcccggg aaagatcggc caaggggaat tggtgggaga taaggggacg
     3061 aagcctgatc acgccctttt tgaccaggtc caaagctatc tgaaaatccc aacaatatcc
     3121 gcgacatccg cgaatttgga tttccttttg cgcaaaaagg ttgaccggga aatggacctc
     3181 atggggggct tcaaataaac cgatgagagc gcagatcccg ccctttttca ggcattgggc
     3241 gctttggtac agggttttct cgaccccaac tgcctcgaaa gatcggtcca cccccaagcc
     3301 accggtttct tccaaaattc ttctcaccgg gtcttccttg gctgcatgga tggctgccgt
     3361 agctcccatc ttcagcgccg tttccagacg atgctccaga agatcagata taaaaacaga
     3421 cccgcatccg ccagccctga ccacctggag cagaaaaaga ccaattcccc cggcgccgaa
     3481 gatcgccgcg ctgtgcccca gccccacttc gcccttgcgg gcggcgtgga cggcaacggc
     3541 cagaggttcc aggagagccc cctcctcgta ggagatggaa tcggggagac ggtggaccca
     3601 gtcttgggag gcgacgaagt aaggagtgaa ccctccctgc ccggcgctgt actggtaccg
     3661 gatattctca cagaggtggt actccccccg gaggcagaag gggcaggttc gacaggtgat
     3721 caccggttca acggcgactc ggtctccaac cgttaccttc gttaccgcgt ggccggcttg
     3781 aatgatctcc ccggagattt cgtggccgat ggccatggga agggaggcca gggggtgctt
     3841 ccccctgtag agatggatgt ctccaccgca gatgctgctg gccttgaccc ggagcagaac
     3901 atcatgatct cctatgggag ggagggtccg tttcacgaaa tccactttcc ccggggcggt
     3961 gaccatggcc atgggataat cttccatcgc aatctccaat cgcagagtgc ttagtgcaaa
     4021 gggcatggcg atcagttttt ctctttgccc tatgctttat actaataacc tgcaggcccc
     4081 ttaaattttt tgtgaagaaa attctcttta cgcgaccccc ttctgtaatc ccccccaccc
     4141 ccctttacca aaggggggcg aggggggatt tctggggggc gcgttgtgcg aggcaagaca
     4201 tatcttttgg ttgcggcttt accgcgctgc ccacaatgcg ctttcttatt ccattatgaa
     4261 attcttcttc ggcccgaacc ttctcggaag ccccatgatc tcccgtacct ggtcccccat
     4321 gagaaaagcc gggggagggc agccgggcac gtagttgggg agatgcttgt attgtttttg
     4381 acagtttccg atgggaagct ggatgtaatc cgcagagccg gaaaggcgcg cttcggaacc
     4441 cacgctgatt tccacccttg ggagcttctc caaggaaatt ccggccgctt taagccgccc
     4501 catggcaatt cgcaggccat tgggacaagc gttacaggcc tgcccgttga ggaaatgtac
     4561 tttttccgca tccaactcga gcggagcgca ggcgcggaca aaaggacggc gaacctcttc
     4621 gatagacctt cccacgaccg ttatgtcctt ttcatcccat acgcccaagc ccataccctg
     4681 tgccaaacag agcgacagaa cttcagtcgg atcaattccc atgacccggg ccgcgatggt
     4741 atccacggcc acggcgtcgg tcccggccat taggatattc agcccgaccg ggctcccggc
     4801 gaccggtcca tccccctcca tggcaataag cccgtccacc acgttcaggt ggggcttcaa
     4861 aaccgcgttc agctccgtaa aggctttgtt gaggccgagg taatggaacc gcttcttctc
     4921 atcataagtg gtcgttccct tcagattctt catggcggcc gtaagggtgc tggcgaaatg
     4981 ggtcttgagc accggggcgt tgatgaagaa atcggccctc aggacctccc tcgaaacccg
     5041 gacgctttcc agcagacccc cagatggggt tttgatttcc tcgaattcgc cgtcgtagag
     5101 gtccagaagc cgggcgccgg tggtccgggc catttcctca tatccgttgg ccgcgaaagc
     5161 tttcttggtg ttcaacccta tggctatcgc ttcggcaatg atcacttccg ccggtctggc
     5221 cttctgcact tcttccacga gggcctgcat gaagaccggg cccgtcagaa tccccttctc
     5281 ccagaggctc tcttcggcgg tgaggttggg cttgatgagg acgcggcttt tgggcgggat
     5341 tagtttcgga gccgggccga tgagggaaaa caactctccc atcatcttcc gaacctcttt
     5401 cagctctttt ccgt
//
LOCUS       DASPRR010000409         5101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1224693, whole genome shotgun sequence.
ACCESSION   DASPRR010000409 DASPRR010000000
VERSION     DASPRR010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224693"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VEL68_15005"
     CDS             complement(<1..713)
                     /locus_tag="VEL68_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ37329.1"
                     /translation="MKFKVPITDYVSSPAVYEEKVLSPIADVPCLEQIHESGLMGKIE
                     DADALIVFHTIRVSALSIRRLQKCRAIVRCGVGYDNVDLAAAGEKGIYVCNVPDYGTD
                     EVADHAIALMLACARGLTFTERRLRKTLVPWNYLAATPLRRLAGSTLGIIGLGRIGTA
                     TALRAKALRMKVVANDPYLADGYDKALGVPLLPLDELLAVSDVVSVHTPLNPETTGIV
                     GAPQLTRMQPHAILVNTARG"
     gene            957..2426
                     /locus_tag="VEL68_15010"
     CDS             957..2426
                     /locus_tag="VEL68_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ37330.1"
                     /translation="MKDLNPLLKPRTIAVVGASERFGAGSLVIENLQALGFQGKVIPI
                     NPRYRELFGFPCFPSLAEVPGDEKIDCVAILLGYEQVIPVLGQAARKNIRGVWALASG
                     FAETGPEGAKLQEEVKRICAENEIQFCGPNCIGYANLRDRVGTYSAPISPTLRKGGIS
                     AVAQSGSVILALANSNRGIGFHFLISSGNEAVVDLTDYIEYLLEDDRTEVIITFIETI
                     RRPEAFIRACTRAAVLGKPIIAVKVGRSEMARRVALSHTGALAGVDGIQEALFKKLGV
                     IRVDDLDQLLETAEIFARCRKRQPKGNGVGAIAVSGGEIGLISDLAEGLSLRFVPLSS
                     NAQVELRKRLPSFTAIGNPLDGWGGGDLVETYPACLEVLAKEKEVDLIVVSQDSSPGM
                     AEKQVNQYSGVARAAVKVAGSGKPVVAISHVSGGLDATIRNILEEGSIPFLQGTREGL
                     LAIHHLIEYAKFQKNRKCKMKEGPPNHLEFSIFFKVRQG"
     gene            2472..4262
                     /gene="iorA"
                     /locus_tag="VEL68_15015"
     CDS             2472..4262
                     /gene="iorA"
                     /locus_tag="VEL68_15015"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HXZ37331.1"
                     /translation="MKVLLSGNEAIARGAYEAGIQITAGYPGTPSSEILENMTQYKQV
                     YSEWSVNEKVAMDMAAGAAYSGRRAMVTTKQVGMNVLSDSLFYSVYTGLEAGLVVVTA
                     DDPGMFSSQNEQDNRHYAKLGKFPLLEPADSQEAKDFVVKGIEISEKFDTPVLIRTTM
                     RTSHSKSVVTLGERPKEKGKVGPFPRNMEKYNCMCLYAKKRHPIVEQRLLDLSAYAEA
                     FPWNRIEWGSRELGIISSGVVFEYAKEVFPDASFLKLGMTFPLPRNLIRLFAQGVRKI
                     LVIEELDPFLEEQIKAMGVQAIGKEYFPPCDELLPQTIRKIARGKGLLPNPEAEPKGA
                     EIPVALPSRSPVLCPGCPHRTSFYLLNKMKLPVAGDIGCYNLGTLPPFSAQHTMGAMG
                     ASIGVLHGMGKSRLPEHPVCTIGDSTFFHAGIPPLLNMVHNKGIGTVIIMDNRTTAMT
                     GHQDHPGIDKTLMGETTKRVDIYGLVKAIGVEKVLQVNAFDVGAMEEALKECIAFPGA
                     SVLIAEGDCVFISKTTGPAHEVDLDLCTACGLCAKLSCPAIIRVDAVHEKTKKKKSAI
                     HPVLCNGCDVCAQVCPTKAIRKGTESKGYL"
     gene            4272..4859
                     /locus_tag="VEL68_15020"
     CDS             4272..4859
                     /locus_tag="VEL68_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXZ37332.1"
                     /translation="MTKKGLKFLISGVGGQGTVLASDILSDVGISSGYDVKKSDILGL
                     AVRGGAVVSHVRWAEKVYTPVMEEGSADYLIGFEWLETLRRIAYVHPGGTILVNDCRM
                     DPISVSSGEAEYPDRERILTLLEKTAKNVVVIPGLQTALDLKEARVLNIVMMGALSGL
                     LATDPKFWEEVIQRRVPKRFLDLNREAYRRGRALV"
BASE COUNT         1209 a         1364 c         1521 g         1007 t
ORIGIN      
        1 cccctggctg tattcaccag gatggcatgc ggctgcatcc gggtcagctg cggagcccca
       61 acgattccgg tcgtttccgg gttcaagggg gtgtgcaccg aaaccacgtc gctcaccgcc
      121 agaagttcgt caaggggaag caggggcaca cccagggctt tgtcgtaccc gtcggcgagg
      181 tagggatcgt tggccacaac tttcatccgg agggccttgg cccgcagggc ggtggccgtc
      241 ccgatccttc ctagtccaat gatccccaag gtcgatccgg caagcctccg gaggggagtt
      301 gcagccaggt aattccaggg gaccagggtc ttccgaagcc gtcgctccgt gaaagtcaat
      361 cccctggcac aggccagcat gagagcaatg gcgtggtcgg ccacttcatc ggttccgtag
      421 tcgggaacat tgcatacata gatgcctttt tcgccggcag cggcgaggtc cacattatcg
      481 tagcccaccc cgcagcggac gatggcccgg catttctgga ggcgccggat ggaaagggcg
      541 gaaacccgga tggtgtggaa aacgatcaaa gcgtcggcgt cttcgatttt tcccataagc
      601 ccggattcat gaatctgttc aagacagggc acatcagcga tcggggataa gaccttttcc
      661 tcataaacgg caggggagga aacataatcg gtgatgggga ctttgaattt catccgcggg
      721 tccctggagg aaatttttca agaatatgaa attctaccgc gggaaggcaa aaaaggaaaa
      781 gaataaaact ccttctgccc ttactaattg actttcccaa acgatgttcc taatattgaa
      841 tccattatag caggatactg cccccttcct ttgaggagat aaaacgggac gtcgcccccg
      901 gagtctaggg ggggatttct cgggtcggtc ctgcgatgat gaatgacgga aatgagatga
      961 aagatttgaa tccccttttg aagccgagaa ccattgccgt ggtaggggcc tcggagaggt
     1021 tcggggccgg gtccttggtg atcgaaaatt tgcaggcgtt gggtttccag ggcaaggtca
     1081 ttccgatcaa tccccgatac agggaattgt tcggattccc ctgttttcca tctcttgccg
     1141 aggttcccgg cgatgagaag atcgattgcg tggcgatcct tctcggttac gagcaggtca
     1201 ttccggttct tgggcaggcg gccagaaaaa atatccgcgg agtctgggcc ctcgccagcg
     1261 ggtttgccga aaccggtccc gagggggcga agcttcagga ggaggtgaag cggatctgcg
     1321 ccgaaaatga gattcaattc tgtggcccca actgcatcgg ctacgccaac cttcgggacc
     1381 gcgtggggac ctacagcgct ccgatttctc ccaccctgcg gaaaggcggg atcagcgcag
     1441 tcgcccagag cgggtcggtc atcctcgcct tagccaacag caacaggggg atcggcttcc
     1501 atttcctgat ctccagcggc aacgaagcgg tcgtggacct gaccgattat atcgaatacc
     1561 ttctggaaga cgatcggact gaagtcatta tcaccttcat cgaaaccatt cggcgtcctg
     1621 aggcctttat tcgggcatgc acacgagccg cagtgttggg caaacccatc atcgcggtca
     1681 aagtggggcg ttcggaaatg gcccggcgag tagctttgtc ccacaccggg gccctggcgg
     1741 gggtggatgg gattcaggaa gccttgttca aaaaactggg ggtcatccgg gtggacgacc
     1801 tggatcagct cctggagacg gcagagattt tcgcccgttg ccgaaaacgc cagcccaagg
     1861 ggaatggagt tggcgcaatt gccgtctcgg ggggggaaat cggcctaatc agcgatctcg
     1921 ccgaagggct ttcccttcga ttcgttccct tatcctcaaa tgcccaggtt gaactgagaa
     1981 agcggctccc ctcctttact gcaatcggga accccctcga cggctggggg ggcggagacc
     2041 ttgtggaaac ataccccgcc tgcctggaag tcttggccaa agagaaggaa gtggacttga
     2101 tcgtggtgtc ccaggattca tcgccgggaa tggcggagaa acaagtgaat caatactccg
     2161 gagtggcccg ggccgcagtc aaagtggcgg gaagcggaaa accggtagtc gccatcagtc
     2221 acgtttcggg gggcctggat gcgacgatcc ggaacatcct ggaagagggg agcatcccct
     2281 ttctccaggg aacccgggag ggcctcctgg ccatccacca tctgatcgaa tacgccaagt
     2341 ttcagaaaaa tcgcaagtgt aaaatgaagg agggtccccc caaccatcta gaattttcaa
     2401 tattttttaa ggttcgccaa ggttgaaaga agttttctga aatgcataaa atctgcatga
     2461 ggagcaactc catgaaagtt cttttgtctg gaaacgaggc gatcgcccgt ggggcctacg
     2521 aggcagggat acagatcacc gcggggtatc ctgggacgcc gagttccgag atcctcgaaa
     2581 atatgaccca atacaagcag gtctattccg aatggtcagt gaatgagaaa gtggccatgg
     2641 atatggccgc cggagccgct tactccggaa ggcgggccat ggtgaccacc aagcaggtgg
     2701 ggatgaatgt tctctccgac tccctctttt acagcgttta cacgggcctg gaggccgggc
     2761 tcgtggtcgt gacggccgat gacccgggga tgttcagcag ccagaacgag caggataacc
     2821 ggcattacgc caagctcggg aaatttccgc tcctggagcc ggccgacagt caggaagcaa
     2881 aagattttgt ggtgaagggg atcgagatca gtgagaaatt cgataccccg gtgctgattc
     2941 ggacgactat gcggacctct cattccaagt ccgtggttac cctgggggaa agacccaagg
     3001 agaaggggaa agtaggtcct ttcccccgaa acatggaaaa atacaactgc atgtgcctgt
     3061 atgccaagaa acggcacccc atcgtcgagc aaaggctttt ggacctctcc gcctacgccg
     3121 aggcctttcc ctggaaccgg atcgagtggg ggagccggga actcgggatc atttccagcg
     3181 gagtggtttt cgaatatgcc aaagaggttt tcccggacgc ctcctttctg aagctgggca
     3241 tgacctttcc tttgccgagg aatctgattc gcctgtttgc ccagggggtc agaaaaatcc
     3301 tggtcatcga ggaactggac cctttcctag aggaacagat caaagccatg ggggtccagg
     3361 caatcggcaa ggaatacttc ccgccctgcg atgagcttct cccccagacc attcgaaaaa
     3421 ttgcccgagg aaagggcctt ttgccgaacc ccgaagccga gccgaaaggc gcggagattc
     3481 ccgtggccct tcccagccgc agcccggtcc tctgtccagg atgcccccac cggacctcct
     3541 tctaccttct gaacaaaatg aaactgccgg tggcagggga cattgggtgc tacaacctcg
     3601 gaacccttcc tcccttcagc gctcagcaca cgatgggtgc catgggggcg agcatcggcg
     3661 ttcttcatgg gatgggcaaa tcgagactgc cggaacatcc cgtatgcacc attggtgatt
     3721 cgaccttttt tcatgccgga attccgccgc tcctgaacat ggtccataat aaaggtattg
     3781 ggacggtcat catcatggac aatcgaacca cagccatgac cggccatcag gaccacccgg
     3841 ggatcgataa aaccctcatg ggagagacca caaagcgggt ggacatttac gggctggtga
     3901 aggccatagg cgtagagaaa gttctccagg tgaacgcctt tgatgtaggg gccatggagg
     3961 aggccctcaa ggaatgtatc gcctttccgg gagcgtccgt tctcatcgcc gagggcgact
     4021 gcgtcttcat ctccaaaacc accgggccgg cccacgaggt tgacctggac ctatgcacgg
     4081 cctgcggcct ttgcgccaag ctgagctgcc cggcgatcat ccgcgtggat gcggtccatg
     4141 aaaagaccaa gaagaaaaaa agcgcaatcc accctgttct ctgcaatggg tgcgacgtgt
     4201 gcgcgcaggt atgtcctaca aaggcaatcc ggaaaggaac ggagagcaaa gggtatcttt
     4261 gaggagttga gatgacaaaa aaaggcctaa aatttttgat ttccggcgta gggggacagg
     4321 ggaccgtcct agccagcgat atcctttccg atgtgggaat ctcttcgggc tacgacgtga
     4381 aaaaatcgga catcctcggg ctggccgtcc gcggaggagc agtggtgagc cacgtccgct
     4441 gggcggaaaa ggtgtacacc cctgtcatgg aggaaggaag cgccgactac ctcatcggct
     4501 ttgaatggct ggagaccctc cgtaggatag cgtatgtcca cccgggcgga acgatcctgg
     4561 tgaacgactg ccgcatggac ccgatcagcg tgagttcggg ggaggcggaa tacccagatc
     4621 gagaaaggat tctgacgctt ctcgaaaaga ccgccaagaa cgttgtggta atccccggcc
     4681 tccagacggc cttggacctg aaggaagccc gggtcctgaa catcgtaatg atgggtgctc
     4741 tcagcgggct gctggcaacc gatccaaaat tctgggaaga agtgatccag agacgggtac
     4801 ccaagagatt tctcgacctt aaccgggaag cttaccggcg gggcagagcc ctggtttaaa
     4861 cggggagggg atagggagta aggaaaaatt tccaagaatg caaggccggc cggccccggt
     4921 agcgcgaagc tgaataagcg aggccgaaca acgtaccgga aaggggagga atatcaagtc
     4981 atgaaaatca tgatcatcaa cccgaacact tcggagagca tgacggagca tatccggaaa
     5041 gaagcggaaa aaatcaaaag acccgacacc gtcttgaccg ttctctgccc cgacaaggga
     5101 c
//
LOCUS       DASPRR010000410         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2190172, whole genome shotgun sequence.
ACCESSION   DASPRR010000410 DASPRR010000000
VERSION     DASPRR010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190172"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="tal"
                     /locus_tag="VEL68_15025"
     CDS             <1..1074
                     /gene="tal"
                     /locus_tag="VEL68_15025"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HXZ37333.1"
                     /translation="RNLINSGGLQRLIYEDGITGITSNPTIFQKAISGSKDYDLSLRR
                     FLAEGMRDEKELFLGLAVEDISGAAQLFWPAYLRSGGLDGYVSIEVSPDLANNTEKSV
                     AEAKRLFSAIGRKNILVKVPGTKEGLPAIEELTFEGVNVNVTLLFSVSRYEEVAEAYL
                     RGLEKRVRQGKPVDEVLSVASFFVSRVDTMVDKLLESRLASAASEGEREKIKSTMGKA
                     AVANAKVAYKKYKEFFGSRRFQSLPKARVQRILWGSTGTKNPQYSDVKYVDELIGRDS
                     INTLPDATLKAYIDHGQTQVTIDKGIREAEGVFSQLSELGIDIDKVTADLERDGVKLF
                     SDSFFALLKEIAQKRDAFLAEKA"
     gene            1071..2468
                     /locus_tag="VEL68_15030"
     CDS             1071..2468
                     /locus_tag="VEL68_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXZ37334.1"
                     /translation="MIARTGRAILGDSPIRNPQSTIRNRLIVFTRYPEPGKSKTRLIP
                     ALGPEGAADLHRQMCEHTLSWARKMRSGSEVSVEIYVDGESQDRFRKWLGDDVPYRPQ
                     GKGSLGARMNRSFQDSFRAGMAKVVVVGTDCPGLSEGPVQKAFLALEENDLVLGPAKD
                     GGYYLIGLRRLAEELFKGIPWGTEKVLAHTLRVAQSLALKTFLLDPLGDVDRPEDLPV
                     WEKFSSTPSLPSPLEGEGKGGGDSAKISIIIPSLNEEENIEVCLASTEGPSRVERIVV
                     DGGSGDRTVEVARSCGARVISSPRGRGRQMNAGARIASGEFILFLHADTRLPAGYADT
                     VRATLSLPGIAAGAFEFRLDATSPSLRFIERVANWRSRRLQLPYGDQAIFLRSSRFQA
                     AGGFPETTIMEDYEFIMGLKKFGRIYTAPYPATTSARRWEKLGIWRATLLNEAVVIAY
                     YLGVSPFALLRLARR"
     gene            2596..3006
                     /locus_tag="VEL68_15035"
     CDS             2596..3006
                     /locus_tag="VEL68_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37335.1"
                     /translation="MNNNLKWVMMSFVSIWALTFLWSGTPVSAQMSGLDGKVFAGELG
                     KKGEKTGDKDDLIFKDGKFVSTACEKYGFGAAPYLVATGEGNTTFEASADGGKDGTMK
                     WKGAIKGDSLTATVVWSKPGQPEQEFWYRGKMKK"
BASE COUNT          703 a          824 c          888 g          619 t
ORIGIN      
        1 aggaatctga tcaactccgg tggactccag cgtctgattt atgaagacgg gataactggg
       61 attacctcca atcctacgat ctttcaaaaa gccatttcgg gttccaaaga ttacgactta
      121 tccctgcgcc gatttctcgc agaaggcatg cgggatgaaa aggagctttt cctgggtttg
      181 gcggtggaag acatatccgg ggccgctcag ttgttctggc cggcttacct tcgaagcggc
      241 ggtctggacg gatacgtcag catagaggtc tcccccgacc tggccaataa cacggagaaa
      301 tccgtggccg aggccaaacg cctcttttcg gccatcggca ggaaaaatat cctggtcaag
      361 gttcccggga ccaaggaggg gcttccggca attgaagaac tgacctttga aggggtgaac
      421 gtgaatgtta ctcttctttt ttcggtcagc cggtatgaag aggtggccga ggcatacctg
      481 cgagggctgg agaagcgggt ccgccaggga aagcccgtcg atgaagtgct gtcggtggcc
      541 agttttttcg tgagccgcgt ggacaccatg gttgataagc ttctggaatc tcgcctggcc
      601 tccgcggcct cagaggggga aagagagaaa atcaaatcca ccatgggcaa ggctgccgtg
      661 gccaatgcca aggtggctta caaaaaatac aaggagttct tcggcagcag gcgtttccag
      721 tccctgccca aggcccgcgt ccagagaatc ctgtggggaa gcacagggac caagaatcct
      781 caatacagcg atgtaaaata tgtggacgaa ttgatcggcc gggattcgat caacaccctt
      841 cctgatgcca ccctgaaagc ttatatcgac catgggcaga ctcaggtcac gatcgataaa
      901 gggatcaggg aggccgaagg ggtattctcg cagttatccg agttgggcat tgatatcgac
      961 aaggttaccg cagacctgga aagggatggg gtcaagcttt tttccgattc cttcttcgcc
     1021 ctcctgaaag agatcgccca gaagagggac gccttcctgg cggaaaaggc ttgatcgcga
     1081 gaacagggag ggcgatcctg ggagattctc caatccgcaa tccacaatcc acaatccgca
     1141 atcggttgat tgtcttcacc cgctaccccg aacctggtaa atccaagacc cgcctgattc
     1201 ctgccctggg ccccgaaggg gcagccgatt tacaccggca aatgtgcgag cataccctct
     1261 cctgggccag gaagatgcgg agcggaagcg aagtctcggt tgaaatttac gtcgacggag
     1321 aaagccaaga ccgcttcagg aaatggctgg gggatgatgt tccttaccgc cctcagggga
     1381 agggaagcct gggggcccga atgaaccggt ctttccagga ttccttccgg gccggaatgg
     1441 ccaaagtggt ggtcgtagga acggattgcc cgggactctc cgaaggaccg gtccagaagg
     1501 cttttttggc gctggaagaa aatgatctgg tgctcggccc ggccaaagac gggggctact
     1561 acctcattgg cctgcgaagg ctggcggaag agcttttcaa aggaatcccc tggggcacgg
     1621 aaaaagtctt ggcccatacc ctaagggttg ctcaaagcct agctcttaag accttcctcc
     1681 tcgacccctt aggtgacgta gaccgccccg aagaccttcc ggtctgggaa aaattctcat
     1741 ccaccccctc cctcccctcc cccctcgagg gggagggtaa gggtggaggg gattccgcta
     1801 aaatctccat catcatcccc tccctgaatg aagaagaaaa catcgaggtc tgcctggcaa
     1861 gcacagaagg cccatcgcga gtggagcgca tcgtagtgga tggaggaagc ggggatcgaa
     1921 ccgtcgaggt cgcccgctcc tgtggggcca gggtcataag cagtccccgg ggacgaggtc
     1981 gccagatgaa cgccggggca cgaatcgcct cgggggagtt cattttattt ctccatgccg
     2041 atacccgtct tcctgcgggt tacgcagata cggtccgggc aaccctgtcc cttccgggca
     2101 tcgctgccgg ggccttcgaa ttccgcctgg atgccacctc ccccagcctg cgcttcatcg
     2161 aacgggtggc gaactggcga tcccgccgcc tgcaacttcc ctacggggat caggccattt
     2221 tcctccggtc ctcccggttc caggccgccg gcgggttccc agagacaacc atcatggaag
     2281 attacgaatt catcatgggc ctaaagaaat tcggccgcat ctacaccgcc ccctacccgg
     2341 ctacaacctc ggcccgccgg tgggaaaaac tgggtatctg gcgagcgacc ctgttgaacg
     2401 aagctgtggt gatcgcctat tacctggggg tttccccctt cgccttactc cggctggccc
     2461 gccggtagtt gggtttaggc cggttatctc cccatcctcg ctgctcctgc ccggaagtcc
     2521 taaaatcgca atcctatcaa tgatcggcac cttacctcca ggtgagtcat ctaaatgggt
     2581 aaggaggtga gaagcatgaa caacaacctt aaatgggtga tgatgtcctt cgtgtccatt
     2641 tgggccttaa cgtttctgtg gagcgggacg cccgtttcgg cccaaatgag cgggctggat
     2701 gggaaagttt ttgccggtga actcggcaaa aaaggggaaa agaccggaga caaagatgac
     2761 ctcattttta aggatggaaa atttgtttct acagcctgtg agaaatatgg atttggggca
     2821 gctccctatt tggtcgcaac gggtgagggg aacacaacct tcgaggcctc ggctgacggc
     2881 ggcaaagacg gaacgatgaa atggaaggga gccatcaagg gggattccct taccgccaca
     2941 gtggtatggt ctaagcccgg ccagcccgag caagagtttt ggtacagggg gaagatgaag
     3001 aaataaggcc gaagacaagc tcaggaatgg ataa
//
LOCUS       DASPRR010000411         8229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_906210, whole genome shotgun sequence.
ACCESSION   DASPRR010000411 DASPRR010000000
VERSION     DASPRR010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906210"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VEL68_15040"
     CDS             complement(<1..894)
                     /locus_tag="VEL68_15040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ37336.1"
                     /translation="MNEKPVGIAIVGIGMWCKALATVMQKSGAFKMVTCFTRTKAKRE
                     EFSAAFQCDHDSSYEDVLKRQDVEAILLTTPNSAHGDMTVMAADHGKHVLVEKPIANR
                     VSDARRMIEACRKNGVVLSVAHNQRRLAGYRKIKEMIQAGSLGKVIMAETNFSHNGGF
                     RLTPQMWRWYEKECPGGPMMTLGVHPAETLQYLLGPVQSVSAFFNRLCLQTEIIDVGA
                     AVLRFELGALGYLGSNFVTPWVNYCNIYGTEANLYLTVELPARKPDETPGQYGNTWNY
                     ADRNSALYLKRKGEDQKVKVDL"
     gene            complement(1481..2716)
                     /locus_tag="VEL68_15045"
     CDS             complement(1481..2716)
                     /locus_tag="VEL68_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXZ37337.1"
                     /translation="MSEFGFMGVDWEERIDFGRMRRERLQRARDAMAKAGADLLLIFS
                     LEDVRYLTGFRSHLGPVALLGMAGAVLPRGGEPLLCTHDIVHARTRMPWIKPENVMDR
                     PFIRTEGGTKKFADEVKAKVGKLAEGKIGVDIWTFGLSQWLPKCFPKAQFVDGREIIN
                     NAKMIKTQDEMECQLIANMITEAGFQALLDNLKPGVKECELLSIAWQKFTELGSEWSQ
                     CSNIVCSGPYTAPYRRFTSDRVIREGDMVIVDIGACFNGYWGDFTRTYVCGNILPTKE
                     QIGLHQESYNTLFNACSEAVVGKTNGDIGKHLNSSTGNPNSDGLSGGHGAGVNPWEPP
                     WVANLSPDAIIPLQEGMLFSIEPYAGKPGIGGFRLENQVFVGKKEPNIFTKLSFDERL
                     VKDVHPLDKSTGRKRAYPK"
     gene            complement(2893..4122)
                     /locus_tag="VEL68_15050"
     CDS             complement(2893..4122)
                     /locus_tag="VEL68_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37338.1"
                     /translation="MSQGAGETPWKVPGKWSVCPYVFEPEVQANWKVPPKVTVHDVTL
                     RDGEQTPGVVFRKEEKLKIAHALEEAGIHRIEGGMIATSQEDFEAIAAMAKEIKNSEV
                     ACFARARRDDMEAAVKSGVKRAVMEITAQDPIIKGIWGSRGKAAEEIIKLVKFGKDSG
                     LKVTYFLMESSRASIDLLRDIIIPVAEEAKPDHIALVDTRGSAYPPAFAWLVTQVRKW
                     VKVPIEIHCHNNWGFATATTLAGVTAGAEVVHTCVNGMGGNAPLDEIIMGLGAFLKVD
                     TGVKTEGFRRLSAMVKDYSRAEWYKPFVGTLTSQVEVGIATRQMWDRRTEHGYGRAEP
                     LNYEVVGGKAIDVVLGKKSGRYSIMLKAYEMGIPMPSEEQATAMLNQIKKISEDNKRL
                     VSDEEFKKIYSQVMGAK"
     gene            complement(4421..5425)
                     /locus_tag="VEL68_15055"
     CDS             complement(4421..5425)
                     /locus_tag="VEL68_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HXZ37339.1"
                     /translation="MPGKVVARAGLDFTIAEKMLAENGGQLAPAPLWTEEDLIRYAAD
                     ADAVIAAATEPYTQRVIQNLRKCKIISRMGIGYNNINVEEATRQGIPVAVVLDASVHE
                     VSDQAMALLLAFSRRLFPLVQAVRKGIWKAGSMEIVKVRGRMFRLNQQTLGLVGVGRI
                     GRRMPAKARAFGMRVLAYDPYLSVQELHQRGAEKADFEQLLSESDYVSIHAPLNPETE
                     KMFGLKEFRKMKPTAVVINTARGAIIDEQALHQALMEGVIAGAGLDVCEPEPPLPDSP
                     LFQLEQVLITGHSAFFSASSTLELQQKATEAVILALRGEWPTVLVNPEVKEQKNRRIG
                     "
     gene            complement(5453..6445)
                     /locus_tag="VEL68_15060"
     CDS             complement(5453..6445)
                     /locus_tag="VEL68_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ37340.1"
                     /translation="MEPKEFDYLLRAQNLKMHFPVKAGIIFDHVVGWVKALDGIDLAI
                     KTGQVVGLVGESGSGKTTLAKVLLLLEKPTEGEVLFRGKGIHSLDDSELKTYRRTLQA
                     VFQDPFASLSPRLRVQEIITEPLRVSSSLGKKELESKALEALHVVGLDTGLRRVYPHE
                     LSGGQRQRVAIARAISTESKIIILDEPTSALDVSVRLQIVHLLMDLQKNLGLSYLLIG
                     HDLAMVAYMSTDIAVMYLGKIVEFGEKKELLQNTAHPYTRALISASLPDHPRLKQERE
                     MISGEIASPLNVPAGCRFHPRCPKKKTVCTELEPCLTAVRKNHWVACHPEEAGT"
     gene            complement(6493..7470)
                     /locus_tag="VEL68_15065"
     CDS             complement(6493..7470)
                     /locus_tag="VEL68_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37341.1"
                     /translation="MEKKPLLQVVGLTTYFFSFARTRIVKAVDNVSFEVYPGDRLALI
                     GESGCGKSTVAASLLRVLPPSGETVSGKVLFEGEDLLQKSNQEMTLVRGKKIGMILQD
                     PMMSLDPVFTIGEQIGETLERHTGLRGDALMRRVKDLLLAVRIPEPERRIRQWPHEMS
                     GGMRQRIVGSIAMSCEPKLLICDEATTNLDVTIQLQYLNLLKDLQKHTGVTLIFITHN
                     LGIVAELCEYVIVMYAGKFMERAPVIDVFDSPAHPYTKALLDAAFGLQDLRKRKPIPG
                     EPPNLALLPPGCSFNPRCEYADDRCRKEEPSEVALGPARSAKCWYPVKG"
     gene            complement(7476..>8229)
                     /locus_tag="VEL68_15070"
     CDS             complement(7476..>8229)
                     /locus_tag="VEL68_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37342.1"
                     /translation="LHDPEIGDPRQRLLPPAWESGGKWTYPLGTDTMGRDVLSRLIYG
                     SRVSLLVAFAAVIIAGCIGTFLGLLAGFFGGWVDQVIMRFTDAWLSIPTVMFGVLMAV
                     ILGPGVWNIVIILGGTFWSRYARVVRGETLSMKTRDFVQLARIAGCGKARIIWKHILP
                     NVFSSVITLSSLQIGIVIVVEASLTFLGVGVPPPKPAWGLMLAEGRGGLLAGYWWLVV
                     FPGVGIGLLVFSFNVIGDWLRRYLDPFYRNIE"
BASE COUNT         1616 a         2543 c         2228 g         1842 t
ORIGIN      
        1 caggtccact tttacctttt ggtcctctcc cttccttttc aggtaaaggg ctgagtttcg
       61 gtcggcataa ttccaggtat tgccgtattg tccgggggtt tcatcgggtt ttcgagccgg
      121 gagctccacg gtcagataaa gattggcctc cgtcccataa atattgcagt aattcaccca
      181 gggggtaaca aagttggagc ccagataccc caaggccccc aattcaaacc gcagaaccgc
      241 cgcccccacg tcgatgatct cggtctgaag gcagaggcgg ttgaagaagg cggaaacgga
      301 ttgaacgggg ccgagaaggt actgaagggt ctctgccgga tggaccccca gggtcatcat
      361 gggacctccg gggcactcct tctcatacca gcgccacatc tggggggtca accggaatcc
      421 gccgttgtgg gaaaagttcg tttccgccat gatgaccttc cccagactcc cggcctggat
      481 catctccttg atcttccgat agccagccag ccttctctgg ttgtgggcca ccgaaaggac
      541 caccccgttt ttccggcagg cctcgatcat ccgcctggcg tcggaaaccc ggttggcgat
      601 gggtttttcc accaggacat gcttcccatg gtccgcggcc atgaccgtca tatcgccatg
      661 ggccgaattg ggcgtagtta ggagaatcgc ttccacgtcc tgccgtttca ggacatcttc
      721 gtaggagctg tcgtgatcgc actggaaagc ggcggagaat tcctcccgct tggctttcgt
      781 ccgcgtgaaa caggtgacca tcttgaaggc cccgcttttc tgcatgaccg tggccagggc
      841 cttgcaccac attccgattc ccacgatggc gattcccaca ggtttttcat tcatggttca
      901 tctcctccaa ccctttttcc ccttctctac cctgaagctg tgaaaaaatt caggcagggg
      961 gaatttgagg agggtgggca tgcccggggg agggacaaat tcacggcgcc aaaacaggcg
     1021 ctggtttctc cgtggccggc ccatcattta attcagtaga gccgaataga gaattctttc
     1081 gttcctatag aagtattccc aagttgccgg tgtcgctttg cggaagtcgg ggaactactt
     1141 cacggccatc catctcccgc caggttttgg cgccgtgaat ttgtccctcc cccgggcaac
     1201 gagaaaggct tctctctccc cctctggagg ccgtgacgtt attcgtcatg cccgcggagg
     1261 cgggcatcca ggcaggcgga gacagcaaca atttcaagga cctggattct gctttcgcgg
     1321 gaataacagg gcttcccctc atcccgacgt agattctgag gcgaagaggg tgaaatcttt
     1381 atgcgatcct gcaaggctgt aggggcaatt catgaattgc ccctacttct cgatcacgaa
     1441 tcgtgactcc ttcgtagccc ctttagggaa accgaccgtt ttatttcggg taggcccttt
     1501 tcctcccggt tgacttatcc aggggatgga catccttcac cagccgctcg tcgaagctca
     1561 gtttagtgaa aatgttgggc tcctttttgc ccacgaaaac ctgattctcc agccggaacc
     1621 caccgatgcc cggtttgccc gcatagggct cgatgctgaa aagcatgcct tcctgcaggg
     1681 ggataatggc gtcgggcgaa agattggcta cccagggggg ttcccagggg tttaccccgg
     1741 ccccgtgacc gcccgacagg ccgtcagaat tggggtttcc cgtcgaggag ttcagatgct
     1801 tgccgatgtc cccgttcgtc ttccccacca ccgcctcgga gcaggcgttg aagagggtgt
     1861 tgtacgattc ctggtggagg ccgatttgct ccttcgtggg caggatattc ccacagacgt
     1921 aggtccgggt gaaatccccc cagtatccgt taaagcaggc gccgatgtcc acgatcacca
     1981 tgtccccttc ccggatgacc cgatcggaag tgaatcggcg gtaaggagcg gtgtaggggc
     2041 cggaacagac gatgttggag cactgactcc attcgcttcc cagctcggtg aacttctgcc
     2101 aggcgatgga caaaagctca cattccttca cccccggttt cagattgtca agcagcgcct
     2161 ggaacccggc ctcggtgatc atgttggcga tcaactggca ctccatttcg tcctgggtct
     2221 tgatcatctt ggcattattg atgatctccc gcccgtcgac gaactgcgct ttggggaagc
     2281 acttgggcag ccactgggag agcccgaaag tccagatgtc cacgccgatc tttccctcgg
     2341 ctaattttcc taccttagcc ttcacctcgt ctgcaaattt cttcgtcccg ccttcggtgc
     2401 ggataaatgg acgatccatc acgttctccg gtttgatcca gggcatgcgc gtccgggcat
     2461 ggacaatgtc atgggtgcag agcagcggtt cccctccgcg gggtaaaacc gctcccgcca
     2521 tacccaggag ggccaccggg ccgaggtggg atcggaaccc ggtgagatat cgcacatcct
     2581 ccagggagaa aataagcaga aggtcggctc ccgccttcgc catggcatct ctggcccgct
     2641 gcaatcgctc ccgccgcatg cggccaaaat cgattcgctc ttcccaatca actcccataa
     2701 atccaaattc cgacatatct acctcctttt attttttgtc cctcaaaaat tgacttgtgc
     2761 gcaagattgc caagaacctg taggggcaac cctttgtggt cgcccatttc ttcgggcggc
     2821 cacagggggc cgcccctaca attttctttt tgcacccggg ttggccaggt taggcaggtg
     2881 aattcctcac cgtcatttcg cgcccatgac ctgggaataa atcttcttga actcctcatc
     2941 gctgaccagc cgcttgttgt cctcggagat cttcttgatc tggttcaaca tggcagtcgc
     3001 ctgttcctcc gagggcatcg ggatgcccat ttcgtaggcc ttgagcatga tcgagtaccg
     3061 gccgctcttt ttccccagca ccacatcgat ggcctttccc cctacgacct cgtaattcag
     3121 gggttcggcg cgcccgtatc cgtgctccgt gcgccggtcc cacatctgtc gggtggcgat
     3181 tcccacctcc acctgggagg tcagagtccc tacgaagggc ttgtaccatt ccgcacggga
     3241 gtagtccttc accatggcgg aaagcctgcg gaagccttcg gtcttgactc cggtgtccac
     3301 cttcaggaaa gctccgaggc ccatgatgat ttcatccagg ggagcattgc cgcccatgcc
     3361 gttgacgcag gtatggacca cttccgcgcc ggcggtgacc cctgccaggg tggtggcggt
     3421 ggcaaatccc cagttattgt ggcagtggat ctcgatgggc accttcaccc attttctcac
     3481 ctgggtcacc agccaggcaa aggccggagg ataggccgag ccccgcgtat caactagggc
     3541 gatatgatcg ggcttcgcct cttcggccac ggggatgata atgtcccgca ggaggtcgat
     3601 gctggcccgg ctgctttcca tgaggaaata agttactttc agcccgctgt cctttccgaa
     3661 ctttaccaat ttgatgatct cttcggcggc tttgccccga ctgccccaga tgcctttgat
     3721 gatcgggtcc tgggccgtga tctccataac cgcccgcttc accccgctct tgaccgccgc
     3781 ctccatgtca tcccggcggg cccgggcaaa gcaggccact tccgagttct tgatttcctt
     3841 ggccatggcc gcgatggctt caaaatcctc ctgggaggtg gcgatcatcc ccccctctat
     3901 gcggtggata cctgcctcct cgagggcatg ggcgatcttc agcttttcct ctttccgaaa
     3961 gaccaccccc ggcgtctgtt ccccgtcccg caaagtcaca tcgtggacag tgaccttcgg
     4021 ggggaccttc cagttggcct gcacctccgg ctcgaagacg taaggacata ctgaccactt
     4081 ccccggaacc ttccagggtg tttcacctgc tccttggctc atttttctct cctctctttt
     4141 ttttaatgca aacgaaatat attattttac cccccaccct cccctccccc tcaagaggct
     4201 gtgtcgtaat cccccgttcg cccttcgaca agctcagggc gaacggctaa gttgctgaat
     4261 tttaatgcgc tcataatccg ttcgtggcga gcttgtcgaa ccatgaacgg attatgacac
     4321 agcctccaag gggggagggt tagggtgggg gtgatgtcgt tttatttaat gcattagtat
     4381 tagtagctcg tagaggaatg atgcatcatg cccctacttt ttacccaatc cgtcgatttt
     4441 tctgctcctt cacctccggg ttgacgagca ccgtgggcca ctccccccgc agagctagga
     4501 tgaccgcctc agtggctttt tgctgaagct ccaatgtgga tgacgccgaa aaaaaggcgg
     4561 aatgtccggt gatcaatacc tgttccagct ggaaaagagg actgtccgga aggggcggtt
     4621 cgggctcaca tacatccagc ccggcgccgg caatcacccc ctccatcagg gcctgatgga
     4681 gggcctgttc atcgatgatg gcccctcggg cggtattgat gacgaccgcg gtgggtttca
     4741 tctttctgaa ttccttcagg ccaaacatct tctccgtctc cgggttgagg ggagcatgga
     4801 tggatacata atccgattcg cttagaagct gttcgaaatc cgccttctcc gctccccgct
     4861 ggtgaagctc ctggacagac aggtaagggt cgtaggccag gaccctcatt ccgaatgctc
     4921 gggctttagc aggcatccgg cgcccgatcc ttcccacccc caccaagccc agggtctgct
     4981 gattcagccg gaacatccgc ccccgcactt tgacgatctc catacttccg gctttccaga
     5041 ttcccttgcg gacggcctgg accaggggga agagtcttcg ggaaaaggcc aacaggaggg
     5101 ccatggcctg gtcggacacc tcgtggacgc tggcatccaa aaccaccgcg acgggaatcc
     5161 cctgtcgggt agcctcttcc acgttgatgt tattgtaccc gattcccatc cggctgatga
     5221 tcttgcactt ccgcaaattc tgaatcaccc gttgggtgta gggctccgtg gcggccgcga
     5281 tcaccgcatc ggcgtcagcg gcatatcgga tgaggtcctc ctcggtccac agcggtgccg
     5341 gggccagttg ccctccgttc tcagccagca ttttctcggc gatggtgaaa tccaggcccg
     5401 ctcgggccac tactttcccg ggcatatctc ctccccggat gattttaaga tgttaggtcc
     5461 cggcctcctc agggtggcag gcgacccaat gatttttcct cacagccgtc aaacaaggtt
     5521 ccagctccgt gcagacggtt tttttcttcg ggcaccgggg atgaaaacga cagccagccg
     5581 gaacattgag cgggctggcg atttctccgg atatcatctc ccgctcctgt ttcagccggg
     5641 gatgatcggg cagggaagcg gaaatcagcg cccgggtgta agggtgcgcg gtgttctgta
     5701 gaagttcctt cttctccccg aactccacga tctttcccag gtacatcacc gcgatgtccg
     5761 tggacatata ggccaccatg gccaggtcat gcccgatgag aaggtagctg agtcccaggt
     5821 tcttctgcaa gtccatcaag aggtggacga tctgcaggcg gacggagaca tccagcgccg
     5881 aagtcggttc gtccaggatg atgatcttcg actcagtaga gatggcccgg gcaatggcca
     5941 cgcgctgccg ttggcctccg ctcagttcat gaggatagac tctccgcaga cccgtgtcca
     6001 gccccaccac gtgcagggct tccagcgctt tgctttccag ttctttcttc cccaggctgc
     6061 tggatacccg caggggctcg gtgatgatct cctgtaccct taggcgtggg ctcagggagg
     6121 cgaaaggatc ctggaagacc gcctgcaggg tgcggcggta agttttcagc tccgaatcat
     6181 ccagggaatg gatgcccttc ccccggaaga gcacctcgcc ctcggtgggt ttctccagaa
     6241 ggagcaggac cttggccagg gtggtcttcc cgctgccgga ctctcctacc agtcctacta
     6301 cttgccccgt cttgatggcc aggtcaatcc catccagggc tttgacccat ccgaccacgt
     6361 gatcgaagat gatcccggct ttcaccggga aatgcatctt cagattctgg gcccgcagca
     6421 aataatcaaa ctcttttggt tccatggagc actgattctt ccatccagaa acttccgcag
     6481 ccctcccgct cctcacccct tcaccggata ccagcatttg gcgcttcggg cggggcccag
     6541 ggccacctcg gacggttctt ccttgcggca acggtcatcg gcgtactcac accgggggtt
     6601 gaagctgcac ccgggaggca gaagggccag attcgggggc tcacccggaa tgggctttcg
     6661 cttcctcagg tcctggagcc caaaggccgc atccagcagg gctttggtat agggatgggc
     6721 cggggaatcg aagacatcaa tcaccggggc tcgttccatg aacttgcccg catacatgac
     6781 gattacatac tcgcacagct cagccacgat cccgaggttg tgggtgatga agatcaacgt
     6841 gaccccggta tgtttctgca ggtctttgag caggttcaaa tattgcagct gaatggtgac
     6901 gtccaggttg gtcgtcgcct cgtcgcaaat cagcagtttc ggctcacagc tcatggcgat
     6961 cgatcccacg atcctctggc gcatcccgcc gctcatctca tggggccact gtcgaatcct
     7021 ccgctccggt tcgggaatcc gaaccgccaa aaggaggtct ttcacccgtc gcatcagggc
     7081 gtcccctcgc aggccagtgt ggcgctccag cgtctccccg atctgctccc cgatggtaaa
     7141 aaccgggtcc agggacatca tggggtcttg caggatcatg ccgatcttct tcccccggac
     7201 cagggtcatc tcctgattgc tcttctgcaa aaggtcttct ccctcgaaga ggactttccc
     7261 gctcaccgtc tcgccggaag ggggcaagac tcgcaggagc gaggcggcta ccgtgctttt
     7321 gccgcaaccc gactcgccga tcaaagccag gcgatccccc ggatagacct cgaaagacac
     7381 gttatccacc gcttttacga ttcgggtgcg ggcgaaggag aagaaatagg tggtcaatcc
     7441 gacgacctgc aacaggggtt ttttttccat ggccctcatt cgatgtttcg ataaaagggg
     7501 tccagatagc ggcggagcca atctccgatc acattaaagg aaaagaccaa taggccgatg
     7561 cccaccccgg gaaagacgac caaccaccag tacccggcca gcaatcctcc cctcccctct
     7621 gcgagcatga gtccccaggc gggcttgggc ggaggaaccc ccactccgag gaaggtgaga
     7681 gaggcttcca caacaatgac gatcccgatt tgtaaagaag agagggtaat cacgctgctg
     7741 aagacgttgg gaaggatatg cttccagatg atgcgagctt tcccgcatcc cgcgattctg
     7801 gccagctgga caaaatcccg ggttttcatg ctcagggtct ccccccggac tacccgggca
     7861 tatctcgacc aaaatgtgcc cccgaggatg atgacgatat tccaaacccc ggggcccaag
     7921 atgaccgcca tgaggacccc gaacatgacg gtgggaatgc tcaaccaggc atcggtaaag
     7981 cgcataatga cctgatccac ccacccccca aagaatccgg ccagcagccc caaaaaagtc
     8041 ccgatgcaac cggcgatgat taccgcggca aaagccacca acagggagac ccttgagccg
     8101 taaatcagcc tgctcagcac atcccgcccc atcgtgtcgg tccccagggg ataggtccat
     8161 ttccccccgc tctcccaggc gggcggcagc aaccgctgcc gggggtcgcc gatctccggg
     8221 tcatggagc
//
LOCUS       DASPRR010000412         9735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1870705, whole genome shotgun sequence.
ACCESSION   DASPRR010000412 DASPRR010000000
VERSION     DASPRR010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870705"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            553..3288
                     /gene="ppdK"
                     /locus_tag="VEL68_15075"
     CDS             553..3288
                     /gene="ppdK"
                     /locus_tag="VEL68_15075"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXZ37343.1"
                     /translation="MAKKYVYFFGAGKAEGSSKMRNLLGGKGCDLAEMTNLKIPVPPG
                     FTITTDVCTSYYANKKKYPKELRDQVQKSLAKVEKAMGVRFGDPRNPLLLSVRSGARV
                     SMPGMMDTILNLGLNEKTIWGLVEMSGDARFAYDSYRRFIQMYSDVVLGVHRGQMEEL
                     ISERKKAKGLRLDIELSADDWKELVGEFKALVKRETGNDFPEDPNKQLWGAIGAVFES
                     WNTPRAITYRHLNRIPEDWGTAVNVQTMVFGNMGGESATGVAFTRDPATGEKFFFGEF
                     LPNAQGEDVVAGIRTPQPIGKEHKADANLLSMEEMLPQAYGELEKIYQRLEKHFKDMQ
                     DIEFTIQQGKLWMLQTRSGKRTAFAALRIAVEMVEEKLIDKKTAISRIDPDSLNQLLA
                     PVFDPQAKAEAVKEGKLLAKGLNAGPGAACGVAVFNPERAMELAKEGKKTVLVRIETS
                     PEDIHGMNAAEGILTSRGGMTSHAALVARGMGKPCVVGCTALNINYGSGTMEVNGRAV
                     REGDYISIDGTTGEVIEGQLPTSSSETIQVLIEKKVRPEDSPIYRQFAKLMSWADEIA
                     DLGVRTNADTPRDATTARLFGATGIGLCRTEHMFFEAERIAAVREMILADTQEERRRA
                     LAKILPMQKKDFIGIFRAMDGYPVIIRTLDPPLHEFLPHTDQEIEELAAQMGVTFRKL
                     KDRAESLKEANPMLGHRGCRLGISHPEITEMQARAIFEAACEVAGEGVKVIPEVMIPL
                     VGHVNELRAQRAIVDRVAKEVMAQTGMKIKYLAGTMIELPRAAITADQVAQVAQFFSY
                     GTNDLTQTVFGLSRDDAGKFLPFYRENGILDSDPFVSIDRPGVGEVIRIGVEKGRKAN
                     PKLEVGICGEHGGDPSSVEFCHMIGLDYVSCSPYRVPIARLAAARAAITYPRRKR"
     gene            3302..4279
                     /locus_tag="VEL68_15080"
     CDS             3302..4279
                     /locus_tag="VEL68_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37344.1"
                     /translation="MYKRAVKIVDARMGILSNNEKLINEPRYFEGQVMESLLTNIHSI
                     EKIPYTDDLDSYFVVMDEPHVQPQVKATAKVCTCTGDFSRYERECSDNRYSLFGNLGL
                     FFKYLLATLERYHNIYSFHASSMYSPSRNTLLMVVGGAGAGKTVFLLKGLEDDWRIFS
                     TEMTHFRFTEKGYEFYMGSLFDNIRLGNLIFDFPRANEKLKVDMPPGVKDVWGHKIAI
                     DMGHIAASRSYVNPRVTVVDAKIESGRDTPICKPVTRMEKVVKILFDNCTEKLGPTLV
                     FYDVIPVESFDTPELMGRRLKVMTRFVQEVKMNPVKSVLAGAKNCMEGI"
     gene            4281..5096
                     /locus_tag="VEL68_15085"
     CDS             4281..5096
                     /locus_tag="VEL68_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HXZ37345.1"
                     /translation="MSRVKSFDGVFEMPVPGRTVKPRKEGLTMIIDKGLGLNGTQDLL
                     DSAADYIDFIKLTFGTSAFLDLDYTAEKVEMVKDYDIWICPGGTFLEVSVWQNTYPKY
                     LKRCKELGFNAIEVSDGTIEIDMKTRADCIKRAVDMGFMVVSEVGKKDPDDKVATSKL
                     HEEIAADLKNGAYKVIVEAREAGKGVGIYDKDGKPKDEEVEAIIKGVDDITKLEWEAP
                     IKNQQQFLILRFGTNVSLGNVPPEDILALEALRNGLRGDTLKKAWLANPKYKK"
     gene            5483..6634
                     /locus_tag="VEL68_15090"
     CDS             5483..6634
                     /locus_tag="VEL68_15090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ37346.1"
                     /translation="MIASFNTVPRILSGFGCLENLGDEITRLKGKKILVISDPGIKAA
                     GLLDPLVKILERTKLSYSLFAEVEPDPKIEIALASHEAARAFGPDLIIGLGGGSSLDI
                     AKVTSAMFTNQGPIDRYFGMELIPEPGLPLILIPTTAGTGSEMTSICVLSDTRNKVKK
                     GIVSEHLFARVALLDPQLTLGLPPRVTALTGMDALVHAVESYTGIRATVLTDTLNLQA
                     IRMIAVNLRKAYANGDNREARENMLYASSIAGMAFSNTQNGLDHALALAIGGKFHLPH
                     GLLTAFILPWVMEFNLLANPQKFIQIARAFGESIDGLSEVEAARLAVRAIKSLLTDLD
                     ISYKLSDYEVPREEIPSLAKAAIGAARLIGNNPRKVTEKEVVEILGKNY"
     gene            6708..7820
                     /locus_tag="VEL68_15095"
     CDS             6708..7820
                     /locus_tag="VEL68_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37347.1"
                     /translation="MRRKDITFGIGLVALGLLFLLATGNSQAQEKIVVGYQPYDTISY
                     QVSVNQELGLWKKYFPKNVEIEFQPALQGSIIVNNMLAGKQQVGYMSIMPATMAMTKP
                     EQANIRIVGSSGMSDGMRCSLLLVRKEAPKFKSQEEAIKWIDGKVVASPKGSAADQYL
                     RMIFDKYKVKPAEYLNQSIEVIATNFRIGKLDAAALWEPTISRICDLVGEGIAKIVAT
                     GTCVNNPDLGTIVMRGDFIDKYPQLAEAWMKSELDAQLFLLDPQNWAKATDMFAKYAT
                     GMPKKALWYSIYGKIPEEVGGTKEREWKPFILDEKVRKNMVDVQAFLFKEKVVKYEKA
                     LPNTIDDSIGRKILQERGLKSPIGYIYGQPPEKVPF"
     gene            7839..8576
                     /locus_tag="VEL68_15100"
     CDS             7839..8576
                     /locus_tag="VEL68_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXZ37348.1"
                     /translation="MQPASQPAKPAAASPNVQISSLGLMLYRLNRWLASPRPYLMFLG
                     LALFIFAWYLFTEVLKLPRFEKVPGPAVAAKEWFSRDPVYGISMFVPEYYQHILKSVL
                     RVLQAFGLATLLGVPLGLFMGWKKTFKDYTFPILETLRPIPMLAWVPLAILMWPLREA
                     AIIYLTFLAAFFATTLNTLLGVESIDEVYFRAARCLGSKPRHIFFRVVIPGSLPFIFT
                     GLQISMGFPGSPWWLEKCSPGNTVWGT"
     gene            8543..8722
                     /locus_tag="VEL68_15105"
     CDS             8543..8722
                     /locus_tag="VEL68_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37349.1"
                     /translation="MLAGEYGLGYLIWNSFIMVQYPIIVIAMVTLGFIGWASSAMIRL
                     VGNLLMKWKVREAAL"
     gene            8722..9609
                     /locus_tag="VEL68_15110"
     CDS             8722..9609
                     /locus_tag="VEL68_15110"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37350.1"
                     /translation="MKEFPKQTAWDSPANKQKDNRTSPAGQLRIEDVTKIYDPEGVNV
                     LALDHCSLRIEAGEFCVVVGPSGCGKTTLLNAIAGFHDITSGEIYLDGQVLCSGKRSA
                     EPGADRIVVFQQGALFPWYTVLENVIYGPVVQGKMDGAEAKNSAERLLGEMGLMGVSH
                     NYPNEISSGMRRRVEIARAMMNDPKVLLLDEPFRALDALTKSVMHKYLLDVYDKSQKT
                     IFFITHDLEEAIFLGTRVVVMTTRPGRIKKMIEVDIPRPRDYRVLSSERYRALKSEAI
                     GAVHEEARKAFEAGERELA"
BASE COUNT         2289 a         2714 c         2780 g         1952 t
ORIGIN      
        1 gccgaagatg tggatcgggt cctgagcggc cagccggctt tgcggtatgt caatttcgcg
       61 gtgccgaaaa agagggccgg cgacgaccgg taaagcaaaa aaccaatggg acgcaggtga
      121 acgcagaaac gggggattac caatcagccc ataattgatt ggacgctgaa tactaagccg
      181 ttcgtgctga gtttgtcgaa gcatgaacgg cgtagagatg cacccttcga caagctcagg
      241 gtcaacggtg ccaggcgaag tgaagagcat ggggttttga cttagaactt gtccgtaaat
      301 aataatcccc ccctttgcaa aggggggttg gggggatttt attaagttga tctaaggcaa
      361 atccccctat gtcccccttt ttcaaagggg gatttaaagg cagatccctt atttaaggat
      421 aagctcgtag atgaacgccg aagaacatgg atgggaaaac gtctaaaatg cataacgtct
      481 ttcgaataat attttatctg cgtttatcta catgaatctg cgtcccaata aaaaatgaga
      541 agaggaagga ttatggctaa aaagtatgtt tattttttcg gcgcaggaaa ggctgagggg
      601 agcagcaaga tgcgcaacct cctcgggggc aaagggtgcg accttgccga gatgaccaac
      661 ttgaagatcc cggttccgcc cgggttcacc atcacgactg atgtctgcac ctcctattat
      721 gccaacaaga aaaagtatcc caaggagctt cgggatcagg ttcagaagag cctggccaaa
      781 gtggagaagg ccatgggggt caggttcggc gatccccgga acccccttct cctttcggtc
      841 cgctccgggg cccgggtttc catgcccggc atgatggaca ccatcttgaa cctcggcctc
      901 aacgaaaaga ccatttgggg cttggtcgag atgtccggcg atgcccggtt cgcctacgac
      961 tcctaccgcc ggtttattca gatgtacagc gacgtagtcc tgggagttca ccggggccag
     1021 atggaagagc tcatcagcga gaggaaaaag gcaaagggcc tcaggctgga catcgaatta
     1081 tcggccgacg actggaaaga gctggtggga gagttcaagg ccctggtaaa aagggagacc
     1141 ggcaatgatt tccctgagga ccctaacaaa cagctctggg gagccatcgg ggcggtcttt
     1201 gagtcctgga acaccccgcg ggccatcact taccgccact tgaacaggat ccccgaggac
     1261 tggggtacgg cggtaaacgt ccagaccatg gtcttcggga acatgggcgg agaaagcgcc
     1321 actggcgtgg ctttcacccg tgacccggcc acaggagaga agtttttctt cggggagttt
     1381 ttgcccaacg cccagggaga agacgtggtg gccgggatcc gcactccgca gcccatcggc
     1441 aaggagcaca aggccgatgc caacctcctc tccatggaag aaatgctccc ccaggcttac
     1501 ggggaacttg agaagattta ccagcggctg gagaagcact tcaaggacat gcaggatatc
     1561 gagttcacca tccagcaggg aaagctctgg atgctccaga cccgcagcgg aaagcggacc
     1621 gcctttgccg cgctgaggat cgccgtggag atggtggaag aaaagctcat cgataagaaa
     1681 acggccatct cccgcatcga tcccgattct ttaaaccagc ttctagcgcc ggtcttcgac
     1741 ccccaggcca aggccgaagc cgtcaaagaa gggaagctcc tggccaaagg gctcaatgcc
     1801 ggaccgggcg cggcctgcgg ggtggcggtc ttcaacccgg agcgggccat ggaactggcc
     1861 aaagagggga agaagaccgt tctcgttcgc atcgaaacct cccccgagga cattcacgga
     1921 atgaacgcag ccgaaggtat cctcacctcc cgcggcggaa tgacctccca cgcggccctg
     1981 gtcgcccggg gcatgggcaa accctgtgtg gtgggttgta cagccctcaa catcaactac
     2041 ggcagcggga ccatggaagt caacggccgc gccgtccggg agggagatta catctccatc
     2101 gacgggacta ctggggaagt aatcgaggga cagcttccca ccagttcatc ggagaccatc
     2161 caggtcctca tcgagaagaa agtccggccc gaggattccc cgatctaccg gcagttcgcc
     2221 aagctcatgt cctgggcgga cgagatcgct gacctggggg tccgcaccaa cgctgacacc
     2281 ccccgcgacg ccacgaccgc ccgcctcttc ggggcgacgg gcatcggcct ctgccggact
     2341 gagcacatgt tcttcgaggc cgagcggatc gccgccgtgc gggagatgat cctggccgac
     2401 acccaggaag agagacgccg tgctctggcc aaaatcttgc ccatgcagaa gaaggacttc
     2461 atcggcattt tccgcgccat ggacggctac ccggtgatca tccgaaccct cgaccccccg
     2521 ctccacgaat ttcttcccca caccgaccag gagattgaag agctggcggc tcagatggga
     2581 gtcaccttcc gcaagctgaa agatcgagcg gagagcctga aagaggccaa ccccatgctc
     2641 gggcaccgag gctgccgtct gggaatcagc cacccggaaa tcaccgagat gcaggcccgg
     2701 gccatcttcg aggcggcctg cgaagtggcc ggggaagggg tgaaggttat ccccgaggtc
     2761 atgatccccc tggtcgggca cgtgaatgag ctccgagccc agcgggcgat cgtggaccgc
     2821 gtggccaagg aagtcatggc ccagacggga atgaagatca agtacctggc cggaaccatg
     2881 attgagcttc cccgggcagc catcaccgcc gaccaggtcg cccaggttgc ccagttcttc
     2941 tcctacggga ccaacgacct gacccagacc gtcttcggcc tctcccggga cgacgccggg
     3001 aaattccttc ccttttaccg ggagaacggg atcctggatt cggacccctt tgtctccatc
     3061 gaccgtcccg gagtgggcga ggtcatccgc atcggcgtgg aaaagggccg taaagccaac
     3121 cccaagctgg aagtggggat ctgcggagaa cacgggggag accccagttc cgttgagttc
     3181 tgccacatga tcgggctgga ttacgtctcc tgctcgccct accgcgtgcc tatcgcccgc
     3241 ctggctgcgg ctcgggcggc cattacctac ccgagaagga agaggtaagc gggaggaagg
     3301 tatgtataag agagccgtaa agatcgtgga tgcccgcatg ggcattctct ccaacaatga
     3361 aaagctcatt aacgaacccc ggtacttcga gggccaggtt atggagtcgc ttctcaccaa
     3421 catccattcc atcgagaaga ttccctatac cgacgacctg gactcctatt tcgtggtgat
     3481 ggatgaacct catgtccagc cccaggtaaa ggccacggca aaagtgtgca cctgcaccgg
     3541 agacttcagc cgctacgaaa gagagtgctc ggataaccgg tactccctct tcggaaacct
     3601 gggactcttc ttcaagtacc tgctggccac cctggagcgg taccacaata tctacagctt
     3661 ccacgccagc tccatgtaca gtcccagccg gaacacgctc ctcatggttg taggcggggc
     3721 tggagctggc aagaccgtct tcctgctcaa gggtctggag gacgactggc ggatcttcag
     3781 cacggagatg acccatttcc ggttcaccga aaaggggtat gagttttaca tggggtccct
     3841 tttcgacaac atccgcctgg gaaacctgat ttttgacttc ccccgggcca acgaaaagct
     3901 caaggtggat atgccccctg gggtgaagga cgtgtggggg cataagatcg ctatcgacat
     3961 gggccacatc gccgcctccc gctcctacgt caacccccgg gtgaccgtgg tggacgccaa
     4021 gattgagtct ggccgggata cccccatctg caagcccgtc acccgaatgg agaaggtcgt
     4081 caagatcctg ttcgacaact gcacagaaaa actaggacct acactggtct tctacgacgt
     4141 gatcccggtg gaaagcttcg acacccccga actcatgggg cgtcgtttga aagttatgac
     4201 ccgttttgta caagaagtga agatgaaccc ggtcaaatcc gtcctagccg gggcaaaaaa
     4261 ttgcatggag ggaatctgac atgagccgcg taaaatcatt cgatggagta ttcgaaatgc
     4321 ccgttccggg gcgcaccgta aagccccgca aagaaggatt gaccatgatc atcgacaagg
     4381 ggctggggct gaacgggacc caggaccttc tcgattcggc cgccgactac atcgacttca
     4441 tcaagctgac cttcggcacg agcgctttcc tggaccttga ttacaccgcc gagaaggtgg
     4501 agatggtgaa ggattatgat atctggattt gccccggggg gaccttcctc gaagtatcgg
     4561 tctggcagaa tacttaccct aaatacctca agcggtgcaa agagttgggg ttcaacgcca
     4621 ttgaggtgtc ggacggcacc attgagatcg atatgaagac ccgggcggac tgcatcaagc
     4681 gcgccgtgga catgggtttc atggtggtct ccgaggtggg gaagaaagac ccggacgaca
     4741 aggtggccac gagcaagcta cacgaggaga tcgccgccga cctgaagaac ggagcataca
     4801 aggtcatcgt ggaagcccgc gaggccggca agggcgtggg gatctacgac aaggacggga
     4861 agcccaagga cgaggaggtg gaggccatca tcaaaggcgt ggacgacatc accaagctgg
     4921 agtgggaagc gcccatcaag aaccagcagc aattcctgat tttgcgattc gggaccaacg
     4981 tgagcctggg caacgttccg ccagaagaca tcctcgccct ggaagccctg cgcaacggtc
     5041 tgcgcgggga cacgctgaaa aaggcctggc tggccaaccc gaagtacaaa aagtgataaa
     5101 tggggcttca gatttgcttg gttaaccgga tgtctcaggg gtggcccatc ggggtcgccc
     5161 ctgcaattgg aagaagagga aggggcctat tcccccaccg ggcgattcgc ccccgttcct
     5221 tccctgccag caggtataac atggtcaaga ctctgcggca ggcagagacc agtgacagaa
     5281 tcactgttcc ttcccaactc ccccccgtga cagcaggaga aatcttcggc caaacccaga
     5341 aaagtgattt gagaagattg tgccccaaca gcgttccggg accgagggtc ggggcggaga
     5401 tggatccccc atccggaaag gcactcgaca aggccgaagt caattccttg tcttttcatc
     5461 cccaaaggga aaggagtctt ccatgatcgc ctctttcaac acggttcccc gcattctttc
     5521 cggcttcggc tgcctggaga acctgggaga tgaaatcacc cgcctcaagg ggaagaaaat
     5581 cctcgtgatt tccgaccccg gcatcaaggc cgctggcctc ctggacccac tggtcaaaat
     5641 attggaacgg accaaactct cctattctct ttttgctgaa gtggagcccg atcctaagat
     5701 cgagatcgcc ttggccagcc atgaggctgc cagggcgttc ggtccagacc tcatcatcgg
     5761 gctcgggggt ggaagctctt tggatattgc caaggtcact tcggcgatgt tcaccaatca
     5821 ggggccgatc gacaggtatt tcgggatgga acttatacct gaaccgggcc ttcctctcat
     5881 cctaatcccc accacggcag ggacgggaag cgaaatgact tcaatctgcg tcctctccga
     5941 cacccggaac aaggtgaaga agggaatcgt gagcgagcac ttgtttgccc gcgtggccct
     6001 cctcgatccc caattgaccc tcggactccc gccgcgggtt accgccctga ccggaatgga
     6061 tgccctcgtt cacgcggtgg aatcgtatac tggaattcgg gcaacggtcc tcacggatac
     6121 cctgaacctc caggccatcc ggatgattgc ggtgaatctg cggaaggcct atgccaacgg
     6181 tgacaaccgg gaagcgcgag aaaatatgct ctacgccagc agcattgcgg gaatggcttt
     6241 ttccaacacc cagaacgggc tcgaccacgc cctggccctg gccattggag gaaaattcca
     6301 cctgccccat ggactgctca ccgcttttat tctcccctgg gtgatggagt tcaacctgct
     6361 ggccaatccc cagaagttca tccagattgc ccgggccttt ggagaaagca tcgacggact
     6421 gtccgaagtg gaagcggcta ggctcgcggt gagggccatt aagagcttgc taacggacct
     6481 ggacatatca tataaattaa gcgattacga agttccccgg gaagagattc cgtctttggc
     6541 caaggcggcc atcggcgcgg ctcgcttgat tggcaacaac ccgcgcaagg tgaccgaaaa
     6601 ggaggtggtc gaaattctgg ggaagaatta ttgattcctt tgtggggaat atccctcttt
     6661 tagtcgagtt tttcaagtcc atcgggaacg gaaaggagaa aaggaagatg aggagaaagg
     6721 atatcacctt tggaattggg ctagtggctt tggggttatt gtttcttttg gccactggga
     6781 attctcaggc tcaggaaaaa attgtggtgg ggtaccagcc ttatgacacc atttcttacc
     6841 aggtctcggt gaaccaggag ctggggctgt ggaagaagta tttccccaag aatgtggaaa
     6901 tcgaatttca gcccgccctt cagggttcga tcatcgtgaa taatatgctg gccggaaaac
     6961 agcaggtcgg ttatatgagc atcatgcccg ccaccatggc catgaccaaa cccgagcagg
     7021 ccaacattcg gatcgtcgga agttccggaa tgtccgatgg gatgcgctgc tcgctgctgc
     7081 tcgtcaggaa agaggccccc aagttcaaat cccaggaaga agcgatcaaa tggatcgacg
     7141 ggaaggtggt cgcctctccc aaagggagcg cagccgatca atatttgcgc atgatcttcg
     7201 ataaatataa agtcaaaccc gcggaatatt tgaaccagtc catcgaagtc atcgccacga
     7261 acttccgcat cggaaaactg gatgccgccg ccttgtggga acccaccatc tccaggatct
     7321 gcgacctggt gggcgaggga atcgccaaga tcgtggctac tggtacctgc gtgaacaacc
     7381 cggacctggg gactattgtc atgcgggggg acttcatcga caaatatccc cagttggccg
     7441 aagcctggat gaaatccgaa ctggatgccc agcttttcct cctggacccc cagaactggg
     7501 cgaaagcgac ggatatgttc gccaaatacg caaccggtat gcccaagaag gccctctggt
     7561 attcaattta cggaaagatt ccggaggagg tgggaggcac taaggaacga gaatggaagc
     7621 cctttatcct ggatgaaaaa gtccgcaaga atatggttga tgttcaggct ttcctgttta
     7681 aggaaaaagt ggtcaaatac gaaaaagccc ttccgaacac gatcgatgat tccatcggcc
     7741 ggaaaatcct ccaggaaagg gggctcaaat cgcccattgg ctatatatat ggacagccgc
     7801 ccgaaaaggt cccattctag aagaagcggg gattgcccat gcaaccagca agccagcctg
     7861 cgaaacctgc cgcggcttcg cccaatgtcc aaatctcatc cctggggctg atgctgtacc
     7921 gcctgaatcg ttggctggcc agccccaggc catacctcat gtttctgggt ctggccctct
     7981 ttatatttgc ctggtacctg tttaccgaag tcctcaaact ccccagattt gagaaagtac
     8041 ccggcccggc ggtggccgcc aaggaatggt tctcacggga cccggtctac gggatttcca
     8101 tgttcgtacc cgagtattac cagcacattc tgaaaagcgt tctccgggtg cttcaggctt
     8161 tcggtctggc cacccttctc ggagttcctc tcggtttgtt catggggtgg aagaagacct
     8221 ttaaagatta caccttcccc atcctggaaa ccctacgtcc cattcccatg ttagcttggg
     8281 ttcctctggc catcttaatg tggcccctcc gggaagcagc cattatctac ctcacctttc
     8341 tggcggcttt ctttgccact acgctcaaca cgcttctggg agtcgaatcg atcgacgaag
     8401 tctattttcg ggccgcccgg tgtttaggct cgaagccccg gcatattttc ttccgcgtgg
     8461 ttatccccgg gtcccttccc ttcatcttta ccggcttgca gatcagcatg gggtttcctg
     8521 gttctccctg gtggctggag aaatgctcgc cggggaatac ggtctggggt acctgatctg
     8581 gaattctttc atcatggtgc aataccccat catcgtgatt gccatggtca ccctggggtt
     8641 catcgggtgg gccagcagtg ccatgatccg gctcgtcggg aaccttctca tgaaatggaa
     8701 agtcagggag gcggcgctgt aatgaaagaa ttcccgaaac aaaccgcctg ggattccccg
     8761 gctaataaac aaaaggataa ccgaacttcc cccgcaggcc agctcaggat cgaggatgtg
     8821 accaaaatct acgacccaga gggggttaat gtcctggctt tggaccactg ttcactccgg
     8881 atcgaggccg gagaattctg cgtggtggtc ggcccctccg gctgcggcaa gacaaccctc
     8941 ctcaacgcca tcgccgggtt tcatgacatc acctcggggg agatttatct ggatgggcaa
     9001 gtcctctgct ccggcaagag gagtgcagaa ccgggggccg accggatcgt tgttttccaa
     9061 cagggggccc tcttcccctg gtacaccgtg ctggaaaatg tcatctatgg gccggtggtc
     9121 cagggcaaga tggacggggc ggaagccaag aattctgcgg agaggcttct gggagagatg
     9181 ggcctgatgg gggtttctca taattacccc aatgaaattt cctccggaat gcgccgacgg
     9241 gtggaaatcg cccgggccat gatgaacgac ccgaaggtcc tacttctgga tgaacctttc
     9301 cgggctctgg atgccctgac caaaagtgtc atgcataagt acttgctgga tgtctacgac
     9361 aagtcccaga agacgatctt ctttatcacc catgacctag aagaagccat cttcttggga
     9421 actcgggtcg tggtgatgac cacccgaccc gggaggatca agaagatgat cgaggttgat
     9481 atccctaggc cccgagatta tcgcgtcttg tcttccgaaa gataccgggc gttgaaaagc
     9541 gaagcgatcg gagccgttca tgaagaggcc cgcaaggcct ttgaggcagg agaaagagaa
     9601 cttgcgtaat ctttcacagc gcagcagagc ctcagccaaa tccccccctc cctaccctcc
     9661 cctctcgagg gggaggggta gggtgggggg gatgcttttt gtttaataaa aatttcagat
     9721 actaaacgtt agtag
//
LOCUS       DASPRR010000413         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_583365, whole genome shotgun sequence.
ACCESSION   DASPRR010000413 DASPRR010000000
VERSION     DASPRR010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583365"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..550
                     /locus_tag="VEL68_15115"
     CDS             218..550
                     /locus_tag="VEL68_15115"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HXZ37351.1"
                     /translation="MEKMLGIYRLFIGPYRLVCSPWESVYISPNRLLMQGAYGQVIEG
                     YGQMGLAVNSPQIMADHTGPELNFAALMGEKIRLGDENFREAAWKFRRNHLERWIPPI
                     HHRPGGWC"
     gene            675..>2001
                     /locus_tag="VEL68_15120"
     CDS             675..>2001
                     /locus_tag="VEL68_15120"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HXZ37352.1"
                     /translation="MREPNSLQKRSPPLRTLQEKRNYLLGAKHQFWVLLLAGTIIIVG
                     SLVWNLTQENYGTLEDARIQARIAYEKDMLYRRWNAGHGTVYVPVTEKTRPDPYLSDL
                     PERDIKTPCGRLLTLMHPTYMTRQAYELAEKESGVYGHITSLKPLRSENAPTLWETAA
                     LQAFERGEKEISSLEQIKGREYLSLMRPLIIEKPCLKCHAKQGYREGQVRGGIRVSIP
                     MAPLRAVQHSHILHFSIAHGLIWLLGLAGISFWTHRLRQSEKRRQWAEEEVRRQNEQL
                     EHSVQERTAELEKANEQLLQDNLERKRAEEALRESEAQLQELLRQRLKVQETERRRIC
                     RELHDELGGGLAVFKLRLNSMAKGLHQDQGKLKEECGDTLKYLDGMIDNVYRISRDLS
                     PYLLEDHGLSAALTWLTDHFRKNYNATVNVNIADMDLGLPRDDQIIIYRI"
BASE COUNT          508 a          497 c          562 g          434 t
ORIGIN      
        1 ttggaatagc agaggtctta agatttcaaa gagttctagg aaacgaatgt ttctcagccg
       61 gcgatattgt aataacttcg tccgggaaat gtcaaaggca aattcttaat ttctgctttt
      121 tttgttgaca gaaataccac aatgtggtat ccaaacttca aatccccggt attgcagcat
      181 tgaataaggt acaaacgaaa ggcaaggaga aaggattatg gaaaaaatgc tggggattta
      241 ccgcctgttt attgggcctt accggctggt ctgctccccc tgggagtcgg tatacatctc
      301 gcccaatcga ctcctgatgc agggagccta tggccaagtc atcgagggtt atgggcaaat
      361 gggcctggcg gtgaatagtc cgcagattat ggccgaccat accgggccgg agttgaattt
      421 cgccgctctt atgggagaaa agattcgcct gggtgatgag aacttccgtg aagctgcctg
      481 gaaatttcgg agaaatcacc tggaacggtg gatcccccca attcaccacc gacctggagg
      541 atggtgctga ttgctgcttc tacagagcct tggccctgga tacaagaaat tttttagaac
      601 gaggatatac gtaaaaactt ccctcatgta agggctcacc accccattgc gggtccggtt
      661 gaaccgaggt aatcgtgaga gagcccaatt ccctccaaaa aagatctcct cccttaagga
      721 ctctgcaaga gaaaagaaat tatcttcttg gggccaaaca ccagttttgg gtcttgctcc
      781 tggccgggac aatcatcatc gttggctctt tggtctggaa tttgacccag gaaaactacg
      841 ggaccctgga ggatgcaaga attcaggccc ggatcgccta cgagaaagac atgctgtacc
      901 ggcgctggaa cgccgggcat ggtacagtct atgtcccggt tacggagaag acgcggcctg
      961 acccctacct ttccgatttg ccggagaggg atatcaagac gccctgcgga cgattactca
     1021 ccctgatgca tcctacctat atgacccgcc aggcttatga gctggctgaa aaagaatccg
     1081 gggtctacgg gcatatcacc agtttgaagc cccttcgttc cgagaacgcc cccacgctgt
     1141 gggaaacggc ggccctccag gcgttcgagc gtggcgagaa ggaaatcagt tccctggaac
     1201 agatcaaggg ccgtgagtac ctgagcctga tgcgccccct gatcattgaa aagccctgtc
     1261 tgaaatgcca tgccaagcag ggatatcggg aagggcaggt tcgggggggg atcagagtct
     1321 cgataccgat ggcccccctc cgtgcggtgc agcattccca tattctgcac ttttctatcg
     1381 cccacgggct gatctggctc ctcggtttgg ccggcatcag tttctggacc caccggctcc
     1441 ggcagagcga aaaaaggcgc cagtgggcag aagaagaggt gaggagacag aatgaacagc
     1501 tggagcattc ggttcaggaa cgaacggccg agctggagaa ggcgaacgag caattgctcc
     1561 aggacaacct cgagaggaaa cgggctgagg aggcgctccg ggaatcggag gctcagcttc
     1621 aagaactgct gaggcagcgc ctgaaggttc aagaaaccga gcgaaggagg atttgtcggg
     1681 aattacatga tgagcttggg ggaggcttgg ccgtctttaa actccggctg aactccatgg
     1741 ccaaagggct ccaccaggac caggggaagc tgaaggagga gtgcggtgat accctaaaat
     1801 atctggacgg aatgattgat aatgtttacc ggatttcccg ggatctaagc ccctatcttc
     1861 tggaagacca tggcttgtca gccgcactca cctggctgac tgaccatttt cggaaaaatt
     1921 ataatgccac ggtgaatgtt aacatcgcag acatggacct ggggttgccc cgggacgatc
     1981 aaattattat ctaccgcatc g
//
LOCUS       DASPRR010000414         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_416131, whole genome shotgun sequence.
ACCESSION   DASPRR010000414 DASPRR010000000
VERSION     DASPRR010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416131"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VEL68_15125"
     CDS             <1..937
                     /locus_tag="VEL68_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37353.1"
                     /translation="PLFIGITIISFGVIHLAPGSPIDMATDLNPKATAEVKERMRAFY
                     GLDQPLHIQYGNWLRRLVALDFGTSFSQDGRKVADKILERLPITIFLNVASLLIILVL
                     AVPIGVFSATHQNSLYDKITTVFVFIGFAVPHFWLALLLMILFGVQLGWLPISGLKSL
                     NYEYLPVFAQVWDRISHLILPLFITASGGLAGLSRYMRSNMLEVIRQDYITTARAKGL
                     ATRTVIFKHAMRNALLPVVTILGLSVPGLIGGSVIFETIFAIPGMGQLFYTSVMARDY
                     PTIMGILVIGAVLTLIGNLIADLSYALADPRIRQT"
     gene            989..1864
                     /gene="opp4C"
                     /locus_tag="VEL68_15130"
     CDS             989..1864
                     /gene="opp4C"
                     /locus_tag="VEL68_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HXZ37354.1"
                     /translation="MEGKRTPNNQAPQDTLSHLFWKRFRKNKLALLGALIVLFLFLVA
                     VLASWISPYDPGQIQIKRVLEEPSPQHPFGTDSLGRDVLSRMIWGSRISLLVGFVAVG
                     IATLIGIFLGSLAGYYGRWIDNLIMRFVDIMLCFPTFFLILAVIAFLEPSIWNIMIVI
                     GATGWMGVARLVRAEFLSLRERDFTVAERALGAKDFRIIFRHILPNALAPVLVSATLG
                     VAGAILTESALSFLGIGVQPPTPSWGNILTAGKDNIEIAWWLSLYPGLAILITVLGYN
                     LLGEGIRDSIDPRLK"
     gene            2009..3418
                     /gene="lpdA"
                     /locus_tag="VEL68_15135"
     CDS             2009..3418
                     /gene="lpdA"
                     /locus_tag="VEL68_15135"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXZ37355.1"
                     /translation="MAERDIVIIGAGPGGYVAAIKAAQMGGKVTLIEKEELGGTCLNW
                     GCIPTKALLRGVELLESVKGGQEFGIQTTGLSVDFPKLMARKDRAVKTLVSGVTALMK
                     TNGIEVIKGKARLLSPQRIQVLDDKGLAVEYQARKVILATGSVSARLPIPGADLTGVI
                     DSNGALQLTRIPESMVVIGAGPIGLEFGTVFAALGTRVNIIEMLPQILPSEDPEVASA
                     LDKALRSFKIQTLTGAQVKMIEETGEGKKKVLTLTEGGEKTFEAEVVLVAVGRKPNLE
                     GLGVQEAGIQVGKKGIEVNSRMETKVPGVYAIGDVTGQWLLAHFAFAQGEVAAENAVG
                     HEAQLEPRAVPRCVYTLPEVASVGLTEKEAREGKTEYRVGRFPFSANGKATVLGERNG
                     FVKIISEAKYGEILGVHIFGPHATDLIGEALLAMQLEGTAQDIALAIHPHPTLTEALK
                     EGALDVDGMALHIPPRKKA"
     gene            3555..>3974
                     /locus_tag="VEL68_15140"
     CDS             3555..>3974
                     /locus_tag="VEL68_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37356.1"
                     /translation="MNFPNLIQPFLLKNLTLRNRIVMAPMLSRLCHPDGIVSQKLIDY
                     YAERAKGGAGLIIVEYCYIDEKESKANQGQLGVYSDQLIAGLGDLAEAIQEWGAKAVL
                     QICHGGRCTSARYMGCQPIAPSAMPSYTGEMAREMTLE"
BASE COUNT          866 a         1080 c         1145 g          883 t
ORIGIN      
        1 tcctctcttc attgggatca ccatcatttc ttttggggtc attcacctag ccccaggctc
       61 ccctatcgac atggccacgg acttgaatcc caaggcgact gcggaggtaa aggaaagaat
      121 gcgggctttt tacggcctgg accagccctt gcatattcaa tacggaaact ggctgcgccg
      181 gctggtcgcc ctggatttcg gcacttcctt ttctcaggat gggagaaagg tagccgacaa
      241 aatcctggag cggcttccca tcacaatttt tctgaacgtt gcctctttgc tgattatcct
      301 tgtactggct gtacccatcg gagtcttctc ggcaacccat caaaactctc tctacgacaa
      361 aataaccacc gtgtttgtct tcatcggctt tgccgtgcct catttctggc ttgcccttct
      421 gttgatgatc ctctttggag ttcagttggg ctggcttccc atctcaggct tgaaatcctt
      481 gaattacgag tacctacccg tgttcgcgca ggtctgggat cggatcagcc atctcatcct
      541 tcctcttttt attaccgcct cggggggtct ggctggattg tcgcggtaca tgcgttccaa
      601 catgctggag gtcattcgcc aggactacat taccacggcc agggccaagg gcctcgcaac
      661 gcgtacggtc attttcaagc atgccatgcg gaatgccctt ttaccggttg tcaccatcct
      721 gggcctttct gtcccggggt tgatcggggg aagcgtcatt tttgaaacca tctttgccat
      781 cccgggcatg ggccagctct tctacaccag cgtcatggcc cgggattatc ccacgattat
      841 gggcatcttg gtgatcgggg cggtgttgac tctgatcggg aatctgattg ccgatctctc
      901 ctatgcccta gccgatccca ggatccggca gacatgagaa aaatcaaatc tctatctcct
      961 tatgcaaaaa gggaatggga ataaagggat ggaagggaaa aggaccccaa acaaccaagc
     1021 cccccaggat accctgagcc acctcttctg gaaaaggttc cggaagaaca aacttgctct
     1081 tctgggggcc ctcatcgtcc tttttctctt cctggttgcg gtcctggcct cctggatttc
     1141 tccatatgat cccggccaga tccagatcaa gcgagtgctg gaagagccaa gcccacagca
     1201 tcccttcggc accgactccc tgggacggga tgttctcagc cggatgatct ggggctcccg
     1261 gatttccctc ctggtaggat ttgtggccgt ggggatcgct accctcatcg gcatttttct
     1321 ggggtctctg gccggctact acggccggtg gattgacaat ctgatcatgc gttttgtgga
     1381 catcatgctc tgcttcccga ctttttttct cattctggcg gtgatcgcct tcctggagcc
     1441 gagcatctgg aacatcatga tcgtcatcgg ggcgacggga tggatggggg tggcccggct
     1501 ggtgcgggcg gaattcctca gcctaaggga acgggacttc acggtggcag aaagggccct
     1561 gggagccaaa gatttccgga tcatcttccg gcatatcttg cccaacgctt tggctcctgt
     1621 attggtttcg gctaccttgg gtgtggccgg ggccattctc actgaatcgg ccctgagctt
     1681 tctgggaatt ggggtgcaac ccccgacgcc cagctggggg aatattctga ctgcgggaaa
     1741 agacaacatc gaaatcgcct ggtggctttc cctgtatccg gggctagcca ttctgatcac
     1801 cgtgctggga tataacctgt tgggggaagg aatccgggat tccatcgacc caaggctaaa
     1861 gtaaaaggcg atttaggagc ggcccaaaga acgggctttg aggagcgctg cgcctgaggc
     1921 aaccgccgat acacggcggc gctgaaaatt gctcatgccc gtaggggcga ttcgggaatc
     1981 gcccccacat gctgatcgga ggaacattat ggcggagaga gacatcgtca ttatcggcgc
     2041 cgggccgggg ggttatgtgg cagccatcaa ggctgctcaa atggggggta aagttaccct
     2101 gatcgaaaag gaagaactcg gcgggacgtg tctgaattgg ggctgcatcc ccaccaaggc
     2161 cctgcttcga ggagtggagc tcctggaatc ggtgaaggga ggccaggaat tcggaattca
     2221 gactaccggg ctatccgttg attttcccaa gctcatggcc cgcaaggacc gggcggtcaa
     2281 gaccctggta tccggggtta cggccttgat gaaaaccaac ggaattgaag tgatcaaagg
     2341 aaaggcccgg ctcctttccc cgcaaagaat acaagtcctg gacgacaagg gactggctgt
     2401 ggaataccag gcccgcaaag tgatcctggc taccgggtcg gtctccgctc ggcttcccat
     2461 tccgggtgcc gacctaaccg gggtgatcga cagcaatgga gcccttcagc ttacccgtat
     2521 ccccgaatcc atggtggtca ttggagccgg gcccatcgga ctggagttcg gtaccgtctt
     2581 tgcggccctg ggcacccggg tcaacatcat agaaatgctt ccccaaatcc tgcccagcga
     2641 ggatcccgag gtggcttcgg ctctggacaa agccttgcgg tccttcaaaa ttcagacctt
     2701 aaccggggct caggtcaaga tgattgaaga gaccggtgaa gggaaaaaga aggttttgac
     2761 cctgaccgag ggaggagaaa agacttttga agccgaggtg gttctggtag ccgtgggcag
     2821 gaagcctaac ctggaaggcc tgggggtcca ggaggccgga attcaggtgg gcaagaaggg
     2881 aatcgaagtc aactcccgga tggaaactaa ggttccaggg gtctatgcca tcggcgatgt
     2941 gaccggacaa tggctcttgg cccacttcgc cttcgcccag ggagaggtgg ccgccgagaa
     3001 tgccgtggga cacgaggctc agctggaacc ccgcgccgtg cctcggtgcg tatataccct
     3061 cccggaggtt gcctccgtgg ggctcacgga aaaggaagcc agggagggga aaaccgaata
     3121 ccgggttggg aggttccctt tttcggccaa cggaaaagcg acggtactcg gagagcgcaa
     3181 tggttttgtg aaaatcattt cggaagcaaa atatggagag attctaggcg tccacatctt
     3241 tggcccccac gccaccgacc tgatcggaga ggcgctcctg gccatgcaac tggaaggcac
     3301 ggcccaggac attgccctgg ctatccatcc ccatccgacg ctcaccgagg cattgaagga
     3361 gggggccctg gatgtggatg gaatggccct gcacattcct ccgcggaaaa aggcatagga
     3421 atgcattcaa cctttacgta aaatgagatt tggagagctg tttacggttg gaaggtctcc
     3481 aatcatccaa caacgggcaa taaacagctg gcaacgagta accagtaacc tgcaaccggc
     3541 aaagaggaat gattatgaat tttcccaatt tgatccagcc ttttctctta aagaacctga
     3601 ccctgcgaaa tcgcatcgta atggccccca tgctttctcg gctttgccat ccggacggga
     3661 tcgtctctca aaaacttatt gattattacg ccgagcgggc caaggggggc gcaggcctga
     3721 tcatcgtgga atattgctat attgacgaaa aggaaagcaa agccaaccag ggccagttgg
     3781 gcgtctattc cgaccagctc atcgccgggt tgggagacct ggctgaagcg attcaggaat
     3841 ggggagccaa ggcagttctg caaatctgcc acggcgggcg ctgcacctcg gcaaggtaca
     3901 tgggatgcca gcccatcgcc ccttcggcca tgcccagcta tactggggaa atggcgcggg
     3961 aaatgactct ggag
//
LOCUS       DASPRR010000415         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2349318, whole genome shotgun sequence.
ACCESSION   DASPRR010000415 DASPRR010000000
VERSION     DASPRR010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349318"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1845)
                     /locus_tag="VEL68_15145"
     CDS             complement(295..1845)
                     /locus_tag="VEL68_15145"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HXZ37357.1"
                     /translation="MNERKPFFLLITVICLCFFLLAPCGAMGSIASAAEPKTQTTSAH
                     STEPVKEGQPSEARHDVQEELGKLLPYWTVAPFVFILLAIALLPLFTSHWWESNLNKG
                     VISTLCSLPILAYLVWIGPIGMEVLAEVLQDYYGFIILLVALFTISGGIHLEGDLKAT
                     PLVNSTFLGLGAILASLIGTTGAAMVLIRPLLSTNRERKYKSHIFIFFIFLVANIGGS
                     LLPVGDPPLFLGYLFGVPFFWTLRLWPLWLTAVAIVLAVFYFWDTRAYSRESAADLRR
                     DRQQQKKLKVHGLINVVLILGVLGSVVLLSDYPMGPYSINLKWMRQPVMILLALISFV
                     LDHRKKERAHKHGHLQFKSPREHNAFTFYAMIEVAVLFIGIFITMTPAICLLKAHGAE
                     TGVKYAWQFFWMSGGLSSFLDNAPTYATYFALGQGVTKGLLASGGGFSVIEANTGPIS
                     ENILMAISAGSVFMGANTYIGNAPNFMVKSICEEAKVKMPTFFGYMAYSIGILIPTFL
                     LVTYIYFL"
     gene            2090..3736
                     /locus_tag="VEL68_15150"
     CDS             2090..3736
                     /locus_tag="VEL68_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXZ37358.1"
                     /translation="MEAKRILEEKKNWEKRCLKKAPEKVQSTSGMGIDILYTPGDVPG
                     MDYSKDLGFPGEYPFTRGIQPTMYRGRVWSMRQYSGFATAEESNKRYKYLLEQGQTGL
                     SVAFDLPTQIGYDSDHPLSAGEVGKVGVAIDSLADMEVLCNGIPLGKVSTSMTINATA
                     ALLMAMYIAVGEKQGVKPGDLRGTIQNDVLKEYVARGTYIFPPQPSMRLITNIFEYCS
                     REVPQWNTISVGAYHIREAGSTAVQEVAFAFAIAIAYLEAGVKAGLDIDDFAPRVSWI
                     FNTHINFLEEVAKFRAARRLWAKIMKERFKAKDPRSWMLRFHTQTGGCTLTAQQPLNN
                     LVRTTFQAMAAVLGGCQSLAVCSYDEALALPTEESVRLSLRTQQIIAHESGLIDSVDP
                     LGGAYYLEELTNGIEKGASEYIAKIDQIGGAVSAIEKGYIQQEVQESSYRYQREIETG
                     NRVLVGVNKFQVKEPPVKGLLKVDPRVREVQVKRIAELKSSRDSRKVQASLAELKKVA
                     RGDGNLMIPILECVRAYSTLGEMCDVLRGVFGEYEPIVTV"
     gene            3861..>4067
                     /locus_tag="VEL68_15155"
     CDS             3861..>4067
                     /locus_tag="VEL68_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911806.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXZ37359.1"
                     /translation="MAERKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEKIASAVVQEDVDALLMSILSGAH"
BASE COUNT          977 a         1048 c         1230 g          812 t
ORIGIN      
        1 gaggcgggcc catcgggcgc ccagtaaggc ggaacgcgtg acgcccctac atcgggatag
       61 ccctgttctt agggaaagaa cggcttgccc gcccggtcca agaaatgggg tgtgcctcat
      121 ggtttttgcg cgcaacgccc tcgctttcgg gccgggcaat gcctgaaatt caatcgatca
      181 tacgcctact tgccaaaaag tgaaggtggg caaggttact tgagtttgcc cttccgaaag
      241 gaagaaaagc cggtcccaaa tccagttctg ccttgtcgcg gattttcaag agaatcatag
      301 aaaataaata taggtgacaa gtaggaaggt ggggatcagg atcccgatgg agtaggccat
      361 gtacccgaag aaggtgggca tcttcacctt ggcttcctca cagatcgatt tcaccataaa
      421 attgggagcg ttcccgatgt acgtattcgc ccccatgaag acggagccgg cagagatggc
      481 catgaggatg ttttcggaga tgggcccggt gttagcctcg attaccgaaa agcccccgcc
      541 ggaagccaac aggcctttgg taactccctg ccccagggcg aaataggtgg cgtaggtcgg
      601 ggcattgtcc agaaaggagc tcaatccccc ggacatccag aagaactgcc aggcatattt
      661 cacgcccgtc tccgctccat gggccttgag gaggcagatg gccggggtca tggtgatgaa
      721 gatgccgata aagagcacgg ccacctcgat catggcgtag aaggtgaagg cattgtgttc
      781 gcgggggctt ttgaactgga ggtgtccatg cttgtgggcc cgctcctttt tcctatggtc
      841 caggacaaaa gagatcaagg ccagaagtat cataaccggc tgccgcatcc atttcaggtt
      901 gatagaataa ggtcccatcg ggtaatcact caaaaggaca accgagccca gaacccccag
      961 gatcaggacc acattgatca agccatggac cttcagcttc ttttgctgtt gcctgtcccg
     1021 ccggaggtct gctgcgcttt ccctggaata agcccgggtg tcccagaaat agaagacggc
     1081 caaaacgatg gccaccgctg tcaaccagag aggccataaa cgcagggtcc agaaaaaggg
     1141 cacgccgaat agataaccca ggaacagggg gggatcgccg accggaagga gagagccgcc
     1201 gatattggcc accaggaaaa tgaagaagat aaagatgtgg ctcttatatt tcctttcccg
     1261 gttggtgctc agcaggggac ggatcagaac catggccgct ccggtggtgc cgataaggct
     1321 ggccaggatg gctcccaaac ccaggaaggt cgaattgacg aggggcgtag ccttgaggtc
     1381 tccctccagg tggatgccgc cggagatggt gaacaaggcg acgaggagaa tgataaagcc
     1441 gtagtaatct tgcaggactt cggccagaac ttccatccct atgggcccta tccagacgag
     1501 gtaggccagg ataggcaggg agcagagcgt gctgatgact cctttattca ggttggattc
     1561 ccaccagtgg ctggtaaaaa ggggaaggag agcaatagcc agtaggatga agacaaatgg
     1621 agcgaccgtc caatagggga gcagcttccc cagttcttcc tgtacatcgt gcctggcttc
     1681 cgagggttgg ccctctttga cgggctccgt ggagtgagcg gaggtggttt gcgtcttggg
     1741 ctcggccgcg gaggcgatgg aacccatggc cccgcagggg gcgagcagga aaaagcaaag
     1801 gcatattacg gtgatgagga gaaaaaaagg ttttcgttca ttcatcgcct gatttccttt
     1861 cttggctggg catcaagaat tttctatacc ctaaacaggc cggagttgac aagatttttt
     1921 ttcatgaaat agcaagtccg caattgaaaa tccaaggata gaaggggcga gtatattctt
     1981 gtaaattttc tcccgttggt ttaagataac ccggatttcc acatcagctc gcaccatccc
     2041 tggttggtga atcgggtgga agggcgtcaa gacggaggaa gaaggcgaga tggaagcgaa
     2101 gagaattctg gaagagaaga aaaactggga aaaacgctgt ctgaaaaagg caccggaaaa
     2161 agtgcagtcc acttcgggca tgggtattga catcctgtac actcccgggg atgtgccggg
     2221 tatggattat tccaaagacc tgggttttcc gggagaatac cccttcaccc ggggcattca
     2281 gccgaccatg tatcgcgggc gggtctggtc catgcgccag tactcgggtt ttgccactgc
     2341 ggaagaatcc aacaaacgtt ataaatacct cctggagcag ggacagacgg gactttcggt
     2401 ggcctttgat cttcccaccc agatcggcta cgattccgac catccccttt ccgcaggaga
     2461 ggtgggcaag gtgggagtgg ccatcgactc cctggcggac atggaagtct tgtgcaacgg
     2521 aattcccctg ggcaaggtca gcacctccat gaccatcaat gccacggccg cccttctcat
     2581 ggccatgtac atcgccgtgg gtgagaaaca gggggtgaaa ccgggcgacc tgcgggggac
     2641 catccagaac gatgtcctca aggaatacgt ggcccgggga acctatatct tcccgcccca
     2701 gccctccatg cggctgatca ccaacatttt cgaatactgc tcccgggagg ttccccagtg
     2761 gaacacgatc agcgtgggag cctaccacat ccgcgaggcc ggttcgaccg ccgtccagga
     2821 ggtggctttt gcctttgcca tcgccatcgc gtatctggaa gcgggggtga aggccgggct
     2881 ggacatcgac gatttcgccc cccgggtctc ctggatcttc aacactcata tcaatttcct
     2941 ggaagaggta gccaaattca gggccgcccg ccggctgtgg gccaagatca tgaaggagcg
     3001 cttcaaagcc aaagacccgc gctcctggat gctgcgcttc catacccaga ccggaggatg
     3061 cacattgacc gcgcagcagc ccctcaataa cctcgtccgg accacttttc aggccatggc
     3121 cgcggtcctg ggcgggtgcc agtccctggc ggtctgctcc tacgatgagg cgctggccct
     3181 gcccacggaa gaatcggtgc gcctttctct gcgaacccag cagatcatcg cccatgaatc
     3241 gggattgatc gacagtgtag accctctggg gggggcctat tatctggaag aattgaccaa
     3301 cggaatcgaa aaaggagcct ccgaatacat cgccaagatc gaccagatcg gcggcgcggt
     3361 gagcgccatc gagaaaggat acattcagca ggaggtccag gagagttcgt accggtacca
     3421 gcgggaaatc gaaacgggga atcgggtttt ggtgggggtc aataaattcc aagtcaaaga
     3481 acccccggtc aaggggctgc tgaaagtgga cccccgagtc cgcgaagtgc aggtcaagcg
     3541 aatcgccgag ctgaagtctt ctcgggactc gaggaaagtc caggcttcgc tggccgagtt
     3601 gaaaaaagtc gccagagggg atggaaatct gatgatcccc atcctggagt gcgtgcgagc
     3661 ctatagcact ctgggagaaa tgtgcgatgt cctgcggggc gtgttcggcg agtacgaacc
     3721 gattgttacg gtttaaataa ttcattggac gcagaagaac gcagatttgc aggatttgat
     3781 gaggaaaaga ggttagttta tctgcgttcg tctgcgaagg tctgcgtcct attaattctt
     3841 taataaaggg ggttggagac atggcggaaa ggaagattcg ggtactggtg gccaagcctg
     3901 ggttggacgg ccatgaccgg ggggcaaagg taatcgcccg ggcgttgcgc gacgccggga
     3961 tggaggtgat ttacacggga ctgcgccaga cgccggagaa gatcgcctcg gccgtcgtcc
     4021 aagaagacgt ggacgctctc ctcatgagca tcctgtctgg cgcccat
//
LOCUS       DASPRR010000416         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1064299, whole genome shotgun sequence.
ACCESSION   DASPRR010000416 DASPRR010000000
VERSION     DASPRR010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064299"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VEL68_15160"
     CDS             complement(<1..276)
                     /locus_tag="VEL68_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37360.1"
                     /translation="MDRVFMGRVVKDFGMLEEKSFLVGKMKKSLLLVERRGKLKIVFK
                     WSGVALFGASMNYFDLKADSLPKLRQCLDEIQSIASQRSNPAPSGSKP"
     gene            606..1952
                     /locus_tag="VEL68_15165"
                     /pseudo
     CDS             606..1952
                     /locus_tag="VEL68_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
     gene            2090..2473
                     /gene="hisI"
                     /locus_tag="VEL68_15170"
     CDS             2090..2473
                     /gene="hisI"
                     /locus_tag="VEL68_15170"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HXZ37361.1"
                     /translation="MEKVAIDFAKEGGLIPAIVQDWKTGEVLMVAYMNADSWAKTQET
                     GKACFWSRSRKKLWLKGETSGHLQRVKEAYLDCDNDALLLKVEQVGGAACHTGFRSCF
                     HRRIEGDRLEILGEKVFDPKEVYGE"
     gene            2466..3338
                     /gene="hisG"
                     /locus_tag="VEL68_15175"
     CDS             2466..3338
                     /gene="hisG"
                     /locus_tag="VEL68_15175"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXZ37362.1"
                     /translation="MNKLKFGIPKGSLEKATTDLFAKAGWLIKTSSRNYFPTVDDPHL
                     SCAIIRAQEIAHYVEEGVLDAAITGKDWILESGSDVVEVQEMIYSKESPRPARWVLVV
                     TEDSPIQRIEDLQGKKIATELVSFTKKYFAERGISVKVEFSWGTTEAKVVEGLVDAIV
                     DVTETGSTIRAHHLRIVHELMQSTPRLIANKNSWEDPWKKEKTQQIATLLKAALGADR
                     MVGLKMNVPQQNLETIIAILPCLNAPTVASLYQSDWYSVEVVVKEEVVRELIPQLLKK
                     GAEGIIEYALNKVI"
BASE COUNT          981 a         1065 c         1046 g          855 t
ORIGIN      
        1 aggcttggaa ccagatggcg cgggattgga acgctggctg gcgatggatt gaatttcatc
       61 cagacactgg cgaagtttgg gaagggaatc ggcctttaag tcgaagtaat tcatgctggc
      121 tccgaagagg gcaacccccg accatttgaa gacgatcttc agctttcccc tccgctccac
      181 cagaagaaga cttttcttca tcttccctac gagaaaggat ttctcctcca gcatcccaaa
      241 atccttgacc acccggccca taaatactcg atccaaaaaa ctcacccgac ctcctttgca
      301 aggcagccag tttcaaaaag aggaagtctt ccctccaatg aaaaccgggg tctacagatt
      361 taccactcct caaaaaaact ggattccggc gccagctccg gtcccaatcc ggggttcggc
      421 ggaatggccg aaaaaaaggg gtttaggatt ttttacgagt tcatcatcct tgacttttgg
      481 tgatttctat tagcataata ttataaagtt aatccagccg ctcgttcaag cgtatcgtgc
      541 cgattgtcat gactgtatcg taagccaacg ggggttggct tttttttcat ggaagggaac
      601 ccttcatggg agttaaaatt accgatacca cgatgaggga cggacatcag tcccttttgg
      661 ccacgcgcct tcgcaccgcc gacattctac ccatagccga agcgatggat cgggtcggct
      721 ttttttccct ggaatgctgg ggaggggcca cttttgacgc ctgcatccgc tttctgaatg
      781 aagacccgtg ggaacgcctc cgcactctca aagctcatat tccgaacact ccgcttcaga
      841 tgcttctccg cggtcaaaac ctggttgggt accgccatta ccctgacgat gtggtggaag
      901 aattcgttcg cctggccatc aaaaacggaa tcggggtctt tcgcatcttt gacgcgctga
      961 atgacgtccg caacatgacc gcagccatcg ctgcggccaa aaaagggaaa acccatgtgc
     1021 aggggacgat ttcctacacc gttagccccg tgcacgccgt cgaaaccttt gtgcagatgg
     1081 ggattgaact ggaaaagacc ggttgtgatt cgatttgcat caaggatatg gctggcctga
     1141 tcacccccaa agccattacc gaattggccc cggctctgag aaaagcggtg aaggtcccca
     1201 tcagccttca cagccactgc acaagcggcg ttgctccctt cagctaccag gcggcagtgg
     1261 aagcaggggt agatattctg gacaccgccc tgggaccttt cagcgggggt acctcccagc
     1321 cacccaccga ggccatggtc gctgcccttc aggggacagc ttttgatacc ggtttggacc
     1381 tcgaacttct agctcagatc agcgggcgcc tcaccccact gcgggagaag tataaggggc
     1441 ttctagaccc gattgccgag cgcccggaca tcaatgtttt tctgcatcaa atccccgggg
     1501 ggatgctgtc caatctcctc tcccagctca aagagcagaa gaaggcggaa aaatacccgg
     1561 aggtcctgaa agaaattccc cgagtccgga aggacctcgg ttaccctccg ctggtcaccc
     1621 ccacttccca gatcgtggga actcaagcgg tcctcaacgt aatcctcggg aaacgctatg
     1681 ccagtgtcac caaggaggta aaagaatatt gcctggggta ttacggtcgg acccccgccc
     1741 ccatcgatcc tgaaatccaa aagaagatca taggcaaaga aacgcccagc gggtgccggc
     1801 ctgccgacct cctcaagccc caattagata ccctaaaaca ggaagcggtg aagatgggga
     1861 ttttgaaaaa ggaagaagac ttggttactt atgccctcta ccccaacgtc gcccccaagt
     1921 tccttcgggg ggagatcaag gaggaagcgc taccggcggc ttaagggaaa acacagatta
     1981 accacagagg gcacagagaa aattcgggca aaaacaggaa atgctcccga gctcattttt
     2041 taattgctct tttttttgaa tgcggaccta atattttggg ggagtgtata tggaaaaagt
     2101 ggccattgat tttgccaaag agggaggact gattccggcc atcgtccagg actggaagac
     2161 cggcgaggtc ctgatggtgg cttacatgaa cgccgacagc tgggccaaaa cgcaggagac
     2221 gggcaaggcc tgcttctggt cccgctcccg gaagaaactc tggctcaagg gcgagacctc
     2281 ggggcacttg cagagggtca aagaagctta cctggactgc gataacgacg cccttctcct
     2341 gaaagtggag caggtcggcg gggccgcctg ccacaccgga ttccggtcct gcttccaccg
     2401 gaggatcgaa ggggatcgat tagagatttt gggagagaag gtcttcgacc ctaaggaggt
     2461 ctacggtgaa taagctgaag ttcgggattc ccaagggaag cctggagaaa gccacgaccg
     2521 atcttttcgc caaggcgggg tggttaatca aaaccagctc ccggaattat ttccccacgg
     2581 tagatgatcc ccatctatcc tgtgccatca tccgggccca ggagatagcc cattacgtgg
     2641 aggaaggagt cctggacgcg gcaatcactg gaaaagactg gatcctggag agcggcagtg
     2701 atgtggtcga agttcaggag atgatctatt ccaaagaaag cccccggccg gcacgctggg
     2761 tcctggtggt gaccgaagat tctcccattc agcggatcga ggacttgcag ggcaaaaaga
     2821 ttgccaccga attggtctct tttacgaaaa agtatttcgc cgagcgtggg atcagcgtaa
     2881 aagtagagtt ttcctgggga accaccgagg ccaaggtggt cgaggggctg gtggatgcta
     2941 ttgtagatgt gaccgagacc ggctccacca tccgggctca ccatctacgc atcgtccatg
     3001 aacttatgca atcgaccccc cgcctcattg ccaacaagaa ttcctgggag gacccctgga
     3061 agaaggaaaa gacccaacag atagccacct tgctcaaggc cgccctgggg gcggaccgca
     3121 tggtggggct gaagatgaac gtaccccaac aaaacctgga gacgatcatc gccatcctcc
     3181 cctgcctcaa tgctcccacc gttgcctctt tgtaccagag cgactggtac tccgtggaag
     3241 tggtggttaa agaagaagtg gtgagggaac tcattcccca gctcctcaaa aagggggctg
     3301 agggaatcat cgagtatgcc ctgaacaagg tcatctgatt ccttttcatc cgaagcaact
     3361 tgcacaatcg gagttgaaaa tagaattgcg gccattctcc gttctctgta acccttggtt
     3421 tgaagccatt tctttcctca ccgtaaccgg cgttaatctt tctctaagaa tactttcaaa
     3481 tttcgaggcc tcacgcccgg aatgcccggt taagttgaag ctctggaggt ttcccagagg
     3541 agattatcga cggtgaggat aaaccgacca tcccccttag tgagggggca tccatccgtg
     3601 atgcgccaaa gagcttgtgg cgatgcccca ggctgtgaaa aaatcccccc gaaaaatttc
     3661 catattcatc ttgacttatt tactcgatcc gtattaattt acatcaagag ttcgtagcct
     3721 atcctcgcca agaaaaaatt aaagatatcc cagtcctgag cctcggacga gggaatagtt
     3781 ttgttaaaaa gccccggttt tccaagtccc ggacagggga ggccctttgg ggtgttccat
     3841 cgcgcggcag agaacctcct ccgggggagg gtgaatccat gggatcgttc ctgcccgcca
     3901 acgataggag gagaagtttt ccagcgatgg gacgcggatt tcttctt
//
LOCUS       DASPRR010000417         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1391310, whole genome shotgun sequence.
ACCESSION   DASPRR010000417 DASPRR010000000
VERSION     DASPRR010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391310"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1197
                     /locus_tag="VEL68_15180"
     CDS             298..1197
                     /locus_tag="VEL68_15180"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HXZ37363.1"
                     /translation="MAAGMRVYRQAKQPLHLRWELYPVQHEPGGKAQYGGSYMEELNW
                     WKKEKKSSLFNEIKYMIPEILFLRGLKKENRRLWNLSFPFHFGFYLMIGTLALLVLGA
                     FSMVLGFPIGPAQGVLSSLIYYLTILFGFPGLTLGMAGTAALFRRRLSDPALRNYSAF
                     SDYLNLVFIFVFFHISLLAWVFYDHAFDGARAYIYSLLTFGATPVGMAGNRTFLGSLS
                     IVLSSLLIAYLPLTHMSHMFMKYFLYHSVRWGDMPNMTGSRIEGAVLANLGLKPTWAA
                     PHIGADGKKTWAQIATSGVKKEK"
     gene            1194..>2416
                     /locus_tag="VEL68_15185"
     CDS             1194..>2416
                     /locus_tag="VEL68_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ37364.1"
                     /translation="MKAPALRPQDIAKPSRRLAQMRAEELPPLPPPYRDFNTKTLTEL
                     KEEKIANIEASLDGTVAIGIPQPKTKEEEEKYLKAFLSGLRKLFEKENNWTFLQPLLL
                     SMEYCAKCQTCSEACPVFEMSGRQEIYRPTFRAEVLRRIYRKYIKPFGKISAKLAGDI
                     DLNWTAIARLAELAYRCTLCRRCASVCPIGVDNALITHEIRKLFSQELGIAPKEIHDK
                     GTLQHLRVGSTTGMNPMAVKDSMDFIQEEMEEITGYKVNIPWDKEGAEVLLLHNAGEV
                     LAWPENVGAFAIIFQAAGISWTLSSEELGYDVVNYGLWYDDMQMARVAIKHAEIAKRL
                     KVKKIIIGECGHAHKALTVIADRVLTGELNIPRESGLVLLDEIVGSGRLNINPARNDF
                     PVTLHDPCNVVRAMG"
BASE COUNT          543 a          670 c          642 g          561 t
ORIGIN      
        1 aagttcgacg ccttccccat gatcctgaaa gtctgcaaga atgtgggcca gcccggaaac
       61 tgcacctatg accaattcat ggaccccctc caggcctgga aaattgcggg ccttccgaaa
      121 ccaacaaccg aggtttttgc ctatataaag cacaaactgt gatcccatgc tgcttccatg
      181 cctgacgccc tatgactcaa atcattacct aagtttgcga aagcctatat atttttgaaa
      241 tgggagctgt caatgacttt gatttttctt acctatttat gctgagcgat ttttattgtg
      301 gccgccggga tgcgggtcta ccgacaagcg aaacaaccgc tccatttgcg ttgggagctc
      361 taccccgttc agcatgagcc gggagggaaa gctcagtacg gcgggtctta catggaagag
      421 ctgaactggt ggaagaagga gaagaagagt tcccttttca atgaaatcaa atatatgatt
      481 cccgaaatcc tcttcctccg gggcttgaaa aaggagaacc gccggttgtg gaacctttcc
      541 tttcctttcc atttcggctt ttacctgatg atcgggactc tggccctcct ggtcctgggc
      601 gccttttcta tggtattggg atttcccatt ggcccagccc aaggggttct gagttcactg
      661 atctattatc tgaccatcct tttcggtttc cccggtttga ccctcggaat ggcaggcacg
      721 gcggctctct tccgacgccg gctgagcgat ccagcattgc ggaattactc cgccttttcc
      781 gattacctga atctggtttt tatcttcgtc tttttccata tcagccttct ggcttgggtc
      841 ttttatgacc atgctttcga cggggcccgg gcttatatct attcactcct gactttcgga
      901 gccacccctg tgggaatggc cgggaatcga acctttctgg ggtccctgtc cattgttttg
      961 agttctctgc tgatcgccta ccttccgctc acccacatgt cccatatgtt tatgaagtac
     1021 ttcctgtacc actcggtgag atggggggac atgcccaata tgaccgggag ccggatcgaa
     1081 ggcgcggtcc tggcaaacct ggggctcaag cccacctggg cggcgcctca tatcggtgcc
     1141 gacggaaaaa agacctgggc acagatcgcg acctcagggg tgaagaagga aaaatgaaag
     1201 cgcccgcgct caggccccag gacattgcca agccttccag acgcctggcc caaatgcggg
     1261 ccgaggagct tccccctctt cccccgcctt accgggattt caataccaag accctgaccg
     1321 aactaaagga agagaagatc gccaacatag aggcttccct cgacggtacc gtggccatcg
     1381 gtattcccca acccaagacg aaagaagagg aagaaaaata cctgaaagcc tttctctccg
     1441 gcctgcgaaa gctctttgaa aaagagaaca actggacttt tcttcagccc ctcctcctct
     1501 ccatggagta ttgcgcgaag tgccagacct gttccgaggc gtgcccggtc ttcgagatga
     1561 gcggaaggca ggaaatctac cggccaactt tccgagcgga ggtgctgcgt cgcatctacc
     1621 gaaagtatat taagcctttt ggaaagatat cggccaagct ggcgggagat atcgatttga
     1681 actggaccgc catcgcccgg ctagctgaac tggcctaccg ctgcacgctg tgtcgccgct
     1741 gcgcgagcgt ctgtcccata ggggtggaca atgccctcat cacccatgaa atccgcaagc
     1801 ttttttcgca ggaattggga atcgccccaa aggagatcca cgacaagggg accctccagc
     1861 acctccgggt gggttccact acggggatga accccatggc ggtcaaggac agcatggatt
     1921 tcatccagga ggagatggag gagatcaccg ggtacaaggt gaacatcccc tgggacaagg
     1981 agggggccga ggtccttttg ttgcataatg cgggcgaggt tttggcctgg ccggagaacg
     2041 tcggggcttt cgccatcatc ttccaggcgg cggggatttc ctggaccctt tcttctgaag
     2101 aacttgggta cgacgtggtc aattacgggc tatggtatga cgacatgcag atggcccgag
     2161 ttgcgatcaa acatgcggag atcgccaaaa gactgaaggt caaaaagatc atcatcgggg
     2221 aatgcgggca tgcccacaag gctttgacgg tcatcgccga ccgggtgctc accggggagt
     2281 tgaacattcc ccgtgaatcc ggtctcgttc ttttggatga aatcgtaggg agcggaagac
     2341 tgaatatcaa ccctgcgcga aacgattttc ccgtcaccct ccacgacccc tgtaacgtgg
     2401 tccgggccat gggcat
//
LOCUS       DASPRR010000418        12319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_908640, whole genome shotgun sequence.
ACCESSION   DASPRR010000418 DASPRR010000000
VERSION     DASPRR010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12319)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12319
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908640"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VEL68_15190"
     CDS             complement(<1..643)
                     /locus_tag="VEL68_15190"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743290.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXZ37365.1"
                     /translation="MKDKWLGASGLVLNEPLIFEKGAKGRKAYSLPKLDVEKVNPQKA
                     WPQRFIRRELEGFPEMSEVDVVRHFTRLSQWNYGVDSGFYPLGSCTMKYNPKVNEDIA
                     RLPGFAGAHPYQPEEISQGALQLIYELETYLSEVSGMDRVTLQPSAGAQGELTGMLMI
                     RAYLMDRGNPRKKVLVPDTAHGTNPASSSLCGYQVVQVKSDERGRLSPRAIEEK"
     gene            complement(654..1988)
                     /gene="gcvPA"
                     /locus_tag="VEL68_15195"
     CDS             complement(654..1988)
                     /gene="gcvPA"
                     /locus_tag="VEL68_15195"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HXZ37366.1"
                     /translation="MRYLPMADSDVAEMLRMVGISRLEDLADVLPGALRLKRDLNLPP
                     PLSEMELVQKLRSLSEAGKSGKPRISFLGAGAYDHFTPSVVDHVLRRSEFYTAYTPYQ
                     PEVSQGTLQAVFEFQTLICQLTGMDIANASVYDGASALAEAVLMGQRIKGKKKCLLAR
                     SVHPEYRTVAHTYTHPLGLEWVPVSFSQEGSLDLQALEKSLNKDVSSVVIQQPNFFGC
                     LEDVERAAQLAHAHDALLVVAVAEPLSLALLQPPGSLGADIVVGEGQSFGNAVSFGGP
                     YIGFFASKENFLRSMPGRLVGETVDLEGRRGFILAVATREQHIRREKATSNICTNQAL
                     CALAVLVYLTIMGKEGLRELAEINLSKCEYAKRRLAETRPLRFTAPTFNEFVLTLKKD
                     PDQILKKLSEQGIIGGLPLKRFYPEMKNEVLICVTEKHSREDIDRLAQALEG"
     gene            complement(1992..2387)
                     /gene="gcvH"
                     /locus_tag="VEL68_15200"
     CDS             complement(1992..2387)
                     /gene="gcvH"
                     /locus_tag="VEL68_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXZ37367.1"
                     /translation="MNFPKDLRYTKDHEWARLEGNVAVVGITDYAQDKLGSIVFVELP
                     PFGKSVKKGETVVTVDSVKAVAEVYSPLSGKVEEVNESLKDIPEIINQDPYGQGWMVK
                     IKISDPAEWGALMTLEAYQAFVAEEEAKG"
     gene            complement(2704..3597)
                     /locus_tag="VEL68_15205"
     CDS             complement(2704..3597)
                     /locus_tag="VEL68_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ37368.1"
                     /translation="MIQTQEIGPVKIFFGKLPSYPYCNTLLVKGRQTLLVDPACEEKA
                     LRHLAAGERIDFLFNTHYHPDHIRYNPLFDGIEFWTHRDDAPCFRSLDSMAEWVGVKN
                     TPYEQNWKASLVNDFGFRERKEVKEIEDGTALDLGGVLLEFLHFPGHTPGLTGFLIPE
                     FQVLFLADMELSPFGPWYHNQRASIQGIIDSVEKIRKIRTQYYIPSHGQEIFRGDIGL
                     RLDRYLENIESRERKILEALSPPKTLDELASLSLISGFRLSRGQVWFFFERNMVEKHL
                     IRLLEQKKISRVGDKFQRETH"
     gene            complement(3859..4620)
                     /locus_tag="VEL68_15210"
     CDS             complement(3859..4620)
                     /locus_tag="VEL68_15210"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXZ37369.1"
                     /translation="MNAKFDLSGKVAIVTGGGRGIGRAIALGLANSGASVAVASRTQK
                     EVDAVAEEIKKLGGKALAVGTDLTANEQLDNLVHVAVKEFGKIDILVNNAARSFLRSL
                     MDLREDGWDKVFNTNVKAVWLLSRLVARKMMEQKSGRIINITTVGAEKAELGMAAYGC
                     SKAALKMLTRCMAREWAQFGIQVNAVGPGLTRTDFSKPIWSNPEVAKHVSAAIPMGRL
                     AEAEEIVGSVLFLASDAARFITGHSIYVDGGSLTT"
     gene            complement(4690..4941)
                     /locus_tag="VEL68_15215"
     CDS             complement(4690..4941)
                     /locus_tag="VEL68_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37370.1"
                     /translation="MLWWGKFILFSAVALFFLEFGVEEMVRAYLLKNPAEFLASFFSA
                     SFIILISGTLLVGFIWRMILRIRGEKGKGQNWEEDNLEH"
     gene            complement(4943..5482)
                     /locus_tag="VEL68_15220"
     CDS             complement(4943..5482)
                     /locus_tag="VEL68_15220"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HXZ37371.1"
                     /translation="MNKGKPVHTRKVEINTFDLGNHQVLVEGELLDTRPSSLEAKESR
                     ARTIHHLLARVRVQGPDLTITDADAEMKRIPREGCPEALPAIQKLVGLRIISGYTQKV
                     KDLIGDVKGCSHLTNLFLTMGPVAVQGYFAAYGRKGAARTPDNPAFARVLNSCHVWRE
                     DGPYVRSLMTALQKGKKES"
     gene            complement(5516..5941)
                     /locus_tag="VEL68_15225"
     CDS             complement(5516..5941)
                     /locus_tag="VEL68_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37372.1"
                     /translation="MARRIRRGVEIFGKTKKLFQDREGSIHQGAPSLPVDGFPRYDLE
                     TGQSEFPKEILLQNSLKIALQVLQTEQVLPLADLAHRIVEELMMNPNYTYRELLSALK
                     EDPRFRVTSGQLVSLPSVENPADIFFRKIARMKKTRGGR"
     gene            complement(6092..7897)
                     /locus_tag="VEL68_15230"
     CDS             complement(6092..7897)
                     /locus_tag="VEL68_15230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HXZ37373.1"
                     /translation="MEGQAFTWDLTDLYQTPREPSWAVDLNRGAERARRLQASYKGGD
                     LSLLSPAAFFQALKEYESIQEEGIKPFLYASLLFSQDSQNNEYKTLLQKAKEQWNELE
                     NQLLFFRLALIGLPPEKLQEFLSHPPLKAYQHALSFLRRFRPFTRGEKEEEIFSRKNL
                     TGRSAFTTLFDEFTGAFSFRLEAEGEEKELTGSQMLSLLYSSDRSLREKAFRIFLKQH
                     GENHLVLGSIFNALILDAQVEDDLRGYQGPMHRIHLENEIRPEAVELMMEVTEAHYPL
                     AREYFQVKACLLGLPKLANFDLYAPLPSSPKKIPFDEARNLLLQSFQRFHPLFGRIAG
                     EFFEKRWIDAPPRKGKYGGAFCAGMTPSLHPYILLNYTGNLRDVLTLAHEMGHGIHFY
                     LARKQTLMNFDPPLTLAETASVFGELVMTQALLREERDLTVRLALLCIEVEDMIATIF
                     RQNVLTRFEQQAYQSRRDHLLSSEEIGDLWWKANARLFGDSVEMIPEYRWGWAYISHF
                     IHSRFYCYSYIFGELVSLALFQKYQEEGPPFLNRLIRLLERGGSGSPHELLKKAGIDI
                     GREDFWEKGFRVIRQLIDELKSLGPWDRICVGSTS"
     gene            complement(8109..8324)
                     /locus_tag="VEL68_15235"
     CDS             complement(8109..8324)
                     /locus_tag="VEL68_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXZ37374.1"
                     /translation="MFGLGTQELLVILAIVFLIFGAKRLPEIGSGLGKAIKNFKGGVK
                     SIEEGTSEKPPEEMKKEAKDSGGPKPV"
     gene            complement(8449..9090)
                     /locus_tag="VEL68_15240"
     CDS             complement(8449..9090)
                     /locus_tag="VEL68_15240"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HXZ37375.1"
                     /translation="MDEFQELMKARQSVYDLLRCFFLQEPTEALFQALQEENVLKNLA
                     GYHPELDEGVHLLGGIIFSPRVPQLVPDLFLEFTRLFVGPSAVPLYESVYRSQSGLMM
                     QEETLAVRKKYMEAGLVVHPDRSFPDDHIGAELEFVFYLCQKAAEAPKMEELFPWLKL
                     QQVFFQDHLNHWVPSLCDRLFQEADSPYFKGVAKMTKGFIAWDFEEFVSHFFD"
     gene            complement(9180..9830)
                     /gene="mobB"
                     /locus_tag="VEL68_15245"
     CDS             complement(9180..9830)
                     /gene="mobB"
                     /locus_tag="VEL68_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HXZ37376.1"
                     /translation="MIPIVSIVGTSDSGKTTLIEKLVPELNLRGYRVATIKHDVHGFD
                     VDREGKDSWRHKQAGAHTVVISSPQKLALIRDVDHDAELAELRDKYIQDVDIILSEGF
                     KRNSQPKIEVFRKERRRELLCTREDNLVAIASNQPFDIGVPCFDLDDARGIVDLIEEK
                     FLVGKKSPPVQLKVEGKDISLSPFVQKSIIATVRGMVSALRGCESPKNVEIRIREE"
     gene            complement(9908..10897)
                     /locus_tag="VEL68_15250"
     CDS             complement(9908..10897)
                     /locus_tag="VEL68_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXZ37377.1"
                     /translation="MKVYLGEVIHPDAVALLEKHAQVVRPKDHSRQAYLEALKEVDGM
                     VARKVYVGAEEMDLAPKMKIIARHGVGLDSVDLAEATRRGILVTNTPGANRESVAELA
                     LSFMLALARRIPQAQKAMANMPKGDVGIFSALLKQYNLTGIDLEGKSLGIIGTGRIGS
                     TVARKCIAAFDMKVRGYDPYVSAEVMKTFGVEKVERLENLLPKIDFLTVHCPLTQETK
                     GMVGKRELAMMKKGTYVINTARGGIVNEKALLEALNSGPIAAAALDVWEIEPPDPKDP
                     LLNHPQVLGTPHYAGTTEESLYRVGIAVVEEVVRVLRGEPPQYPINPEVLKKK"
     gene            complement(11104..11457)
                     /locus_tag="VEL68_15255"
     CDS             complement(11104..11457)
                     /locus_tag="VEL68_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HXZ37378.1"
                     /translation="MTKQDILRVLDHILDPDLKDRSIVEMGFVREDDIELQEEEIRVS
                     YTVGGPLCPYSAAVGIIIHYTLHERFKKHIKVRMKEGHYQEELVNQILGDEGQYEVWL
                     EKIKSQNLLSACLRA"
     gene            complement(11633..11998)
                     /gene="nifU"
                     /locus_tag="VEL68_15260"
                     /pseudo
     CDS             complement(11633..11998)
                     /gene="nifU"
                     /locus_tag="VEL68_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
     gene            complement(12035..>12319)
                     /locus_tag="VEL68_15265"
     CDS             complement(12035..>12319)
                     /locus_tag="VEL68_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HXZ37379.1"
                     /translation="AVEYIEGEAMLLFMDMEGVRISSGSACISRSLKVSHVMLAMGVD
                     AATAQGSLLFSFGMQNKPQDVDHILQVLPPIVERLRNMSPVYRKAMAKKT"
BASE COUNT         2711 a         3457 c         3249 g         2902 t
ORIGIN      
        1 tcttctcttc gattgctcgg ggggataacc ttccccgctc gtcagatttc acctgaacga
       61 cctggtaccc gcagaggctg gaactggccg ggttggtccc gtgggccgtg tcgggaacca
      121 agaccttttt ccgcggattc ccccggtcca tcaagtacgc gcgaatcata agcatccctg
      181 tcagctcacc ctgagctccg gcggagggct gcagggtcac tcgatccatg ccgcttactt
      241 cactaaggta cgtctccagt tcatagatca attggagggc tccctgggag atttcttcgg
      301 gctggtaggg atgcgccccg gcaaaaccgg gcaagcgcgc gatatcctca tttactttgg
      361 ggttatattt catggtgcaa gagcccaaag gatagaaccc ggaatcgacc ccgtagttcc
      421 actgggagag acgggtgaag tggcggacca catccacctc gctcatctcc gggaaacctt
      481 ccagttcccg gcggatgaac ctctggggcc atgccttttg cgggttgacc ttttccacat
      541 ctagcttggg caaggaatag gcttttctgc ccttcgctcc cttttcaaag atcaggggtt
      601 cgttcaaaac cagcccgctt gcccccagcc atttgtcttt catttttctc ccctcaacct
      661 tccagagctt gggctagccg gtcgatatcc tcgcggctgt gtttttccgt cacgcagatc
      721 aggacttcat tcttcatttc cggatagaac cgcttcaagg ggaggcctcc gatgatcccc
      781 tgctcggaaa gtttcttaag gatttgatcc gggtctttct tcaaggtgag cacaaactcg
      841 ttgaaggtgg gcgccgtgaa ccgcaggggt cgggtctccg ccagccttct cttggcatac
      901 tcgcacttgc tgagattgat ctccgccagc tcccgcaggc cttccttccc catgatggtc
      961 aggtagacga ggaccgccag ggcgcacagg gcctggttgg tgcagatgtt ggaggtggcc
     1021 ttttcccggc ggatgtgctg ttcccgggtg gcgaccgcca ggatgaaccc gcggcgtccc
     1081 tccaaatcca ccgtttcccc caccaatctt cccggcatgg agcgaagaaa attctccttg
     1141 ctggcgaaga acccgatgta aggcccgcca aagctgaccg cattgccaaa actctgcccc
     1201 tctcccacca caatgtccgc cccgagcgat cccggaggct ggagcaaggc cagggagaga
     1261 ggctcggcca ccgccacaac caacagggca tcatgggcat gggccaactg agcggccctc
     1321 tccacatctt ccaggcagcc gaagaaattg ggctgttgaa tgaccaccga actgacatcc
     1381 ttgttcagtg acttttccaa tgcctggagg tccagactcc cctcttgaga gaaagaaacc
     1441 gggacccatt ccaatcccaa aggatgagta taggtatgag cgaccgtccg gtattcggga
     1501 tgaaccgacc gggccaggag gcatttcttt tttcctttaa tccgctggcc catcaacacg
     1561 gcctcggcca gcgcggaggc tccgtcatag accgaggcgt tagcgatatc cattccggtg
     1621 agctgacaga ttagggtctg gaactcgaat acggcctgga gcgttccctg gcttacttcc
     1681 ggctggtacg gagtgtaggc cgtgtaaaat tccgaacggc gtagcacgtg gtccacgacg
     1741 gagggagtaa agtggtcgta ggccccggcc cccaggaagg aaatcctggg tttccccgat
     1801 ttcccggcct cgctgaggga gcggagcttc tggacaagct ccatctccga aagagggggg
     1861 gggaggttca ggtctctttt cagacgcaga gcgccgggga gaacatcggc caaatcctcc
     1921 agccggctga tgcccaccat ccggagcatc tccgccacgt cgctatccgc catcggaagg
     1981 tagcgcattt cctatccttt cgcctcttct tcggccacga aggcctggta ggcctccagg
     2041 gtcatgaggg caccccattc ggcagggtcg gaaatcttga tcttcaccat ccatccctgg
     2101 ccgtagggat cctggttgat gatttccggt atatccttca gggattcatt gacctcctcc
     2161 actttcccgc ttaaagggga atagacctcg gcaacagctt tcaccgagtc cacggtgacc
     2221 accgtttcac ctttcttcac ggacttgccg aacgggggaa gttcgacgaa cacgatactg
     2281 cccagcttgt cctgggcgta gtcagttatc cccactactg ctacattccc ttcaagccgg
     2341 gcccattcgt gatccttggt gtacctcagg tcctttggaa agttcatgaa ttccctccta
     2401 aaataaagcg gtcagcgatc agcactcagc tttcagcaaa caacgactct tgctgatggt
     2461 ttaatgctga tcgctgatag ctgaatgctg atcgctcatt ttttcttcat ccatcccttt
     2521 tcttcagcct tttggcggag acgttccagg gattcaaccg ttggcttttt ccttcccccg
     2581 agttgatccc taacaaagat cttttttttc tccggctttc ggtctttttc tttcatgatg
     2641 ggcgggcaat gaactaaatt ttattccgtc ggagggaaaa gataaaggga aaatatgtat
     2701 ccttcaatgg gtttcccttt ggaatttgtc ccccaccctg gatatcttct tctgttccaa
     2761 caatcgaatc aaatgttttt caaccatgtt cctttcgaag aagaaccaaa cctgtccccg
     2821 ggacagccgg aacccggaga tcaggcttaa ggaagcgagt tcatccaggg tcttcggagg
     2881 agagagggct tcgaggattt tcctttcccg ggattcgatg ttctccaaat accggtccag
     2941 ccgcaggccg atgtcccccc ggaagatttc ctgcccatgg gatgggatgt aatattgagt
     3001 gcggatttta cggatttttt cgaccgaatc gatgatgcct tgaatgctgg cccgttggtt
     3061 atgataccag gggccgaagg gagatagttc catatcggcc agaaaaagaa cctggaattc
     3121 ggggatcaag aaaccggtga ggccgggggt gtgcccggga aagtggagga actcgaggag
     3181 aacacccccc aggtccaagg ccgttccatc ctctatctct ttaacctcct tcctttcccg
     3241 aaacccgaaa tcattcacca gggaagcctt ccagttttgc tcgtaagggg tatttttcac
     3301 ccccacccat tcggccatgg aatcgagaga acggaaacag ggagcgtcgt cccgatgggt
     3361 ccagaattca atgccgtcga aaaggggatt gtagcgaata tggtcggggt ggtaatgggt
     3421 gttgaaaagg aagtcgattc tttctcccgc agcaaggtgc cggagggcct tttcctcgca
     3481 agcggggtcc accagaaggg tctggcggcc cttcaccaaa agggtattgc agtaggggta
     3541 ggagggaagc tttccgaaaa agatttttac cgggccgatt tcctgagttt ggatcatatc
     3601 ctgccaaagg cgaaaccggt tcctttcaga tgggtggagt taaaccgaag cgatgccccc
     3661 ggtcgtcctc catcggagac tgggttatta ttcgtcatgc ccacggatgc gggcatccag
     3721 tcagttgcag acctcgacaa tttccatgaa ctggattccc gctttcgcgg gaatgacttt
     3781 ttatgcctca ttaccacgca gtctctcgag gaggagggag ttggtttcat tgacaagcac
     3841 cgaggcataa cccaaaaatc aagtggtcag gcttcccccg tcgacataga tcgaatggcc
     3901 ggtaataaac cgggcggcgt ccgaggccag gaaaagaacc gaacccacga tctcctcagc
     3961 ctcagccagc cggcccatgg gaatggccgc agaaacgtgc ttcgccacct ccgggttgga
     4021 ccagatgggt ttgctgaaat ccgtcctggt caggccgggg cccacggcat tgacctggat
     4081 tccgaattgg gcccattccc gggccatgca gcgggtgagc atcttcaggg cagccttgct
     4141 gcacccgtag gccgccatgc ccagctcggc cttctccgcc ccgacggtgg taatgttgat
     4201 gatccgcccg ctcttctgct ccatcatctt gcgggccacc agccggctca acagccacac
     4261 cgccttcacg ttggtgttga aaaccttgtc ccacccgtcc tcccgcaggt ccataaggct
     4321 gcgcaggaag ctgcgcgcgg cattgttcac caagatgtcg atcttcccga actctttgac
     4381 cgccacatgg accaggttat ccaattgctc attggcagtg aggtccgtgc ccacagccag
     4441 ggcttttcca cccaatttct taatctcttc ggccaccgca tcgacctctt tctgagtccg
     4501 gctagcaacg gcgaccgagg ccccggaatt agccaacccc agggcaatgg cccggccgat
     4561 gccgcgaccc cctccggtga cgatggccac cttgccgctc aaatcgaatt tggcgttcat
     4621 gattcattcc tcccgtaatg atagtcgggg aaaagggtca agagggaaaa ggcgaaaggt
     4681 gggaaaaagt caatgttcca aattgtcttc ttcccagttt tgccctttcc ctttttcccc
     4741 cctgattctt aagatcatcc tccaaatgaa ccctactagg agggtcccgc tgatcaaaat
     4801 gatgaaactg gcggagaaaa aggaagccag gaactcagcc ggattcttga gaaggtaggc
     4861 ccggaccatc tcctcgaccc caaattccag gaagaaaaga gccacggcag aaaagagaat
     4921 gaacttgccc caccaaagca tcctaggatt ccttttttcc cttctgcagc gcggtcatca
     4981 gagaacggac ataagggcca tcttcccgcc agacatggca actgttgagc acccgggcga
     5041 aggccgggtt gtctggagtc cgggccgccc ctttccttcc gtaggcggcg aagtatccct
     5101 gcacggccac cggtcccata gtcaagaata gattggtcag atgggaacac cctttcacat
     5161 ccccgatcaa gtcttttacc ttctgggtat atccagagat aatccggaga ccgaccaatt
     5221 tttgaattgc cgggagagcc tccggacacc cctccctggg aatccttttc atctctgcat
     5281 ccgcgtcggt gatggtgagg tctggcccct ggacccggac ccgggccaaa aggtgatgga
     5341 tcgttcttgc cctgctctct ttcgcttcga gggaagaggg ccgggtatcc agcagttctc
     5401 cttccactag aacctgatga tttcccaggt caaaagtatt gatttcaacc ttgcgagtat
     5461 ggaccggctt tcctttgttc atttcccttc ccttccatga ataacccccc gggcttcaac
     5521 gaccccccct ggttttcttc atccgggcaa tcttccggaa gaaaatatct gcagggtttt
     5581 ccactgaagg aaggctgacc aactggccgc tggtcaccct aaatcgtggg tcctccttca
     5641 aggcggaaag cagctcccga taggtataat tcgggttcat catcagctcc tccacaattc
     5701 ggtgggccag atcggcaagg gggaggacct gctccgtttg taacacctgt aaggcgattt
     5761 ttaggctatt ctgcaggagg atttccttgg ggaattcaga ttggccggtt tccaggtcat
     5821 agcggggaaa gccgtccacg gggagagaag gggcaccctg gtgaatgctg ccttctcggt
     5881 cttgaaaaag cttcttggtc tttccaaaaa tttccactcc ccgccgaatt ctccgcgcca
     5941 ttagcccttt cctccaactg ccggatccat gtctcctggg gttgattgcc ctgtgatttt
     6001 agcggaatta ttcggagtta ccaataaaaa aattcccttc cctcggcctt ctcctggatc
     6061 aggggagaag cagggagcaa gggggtgggt gttaggaggt cgaccccacg cagattctat
     6121 cccaagggcc aaggcttttg agctcgtcga tgagttgacg gatcacccga aacccctttt
     6181 cccaaaaatc ctcccgcccg atgtcgatcc cggctttctt caaaagctca tgaggactcc
     6241 cggacccacc cctctccagg aggcggatga gccgattcag gaagggaggg ccttcttcct
     6301 ggtatttttg aaacagggcc agggagacca attccccgaa aatataactg taacaataga
     6361 agcgggaatg gatgaaatga gaaatgtagg cccagcccca ccgatattcg gggatcattt
     6421 cgacagagtc cccgaaaagc cgagcattgg ccttccacca aaggtccccg atctcctcgc
     6481 tgctcaagag atggtctcgc cgactttgat aggcctgttg ctcgaagcgg gtcaggacgt
     6541 tctggcgaaa gatggtagcg atcatgtcct cgacctctat gcagagaaga gcgaggcgga
     6601 cggtcaggtc ccgttcttcc cggagaaggg cctgggtcat gactagctcc ccgaatacgg
     6661 aagctgtttc agccagggtg aggggagggt cgaagttcat cagggtttgc ttgcgggcca
     6721 ggtagaaatg aatcccatgc cccatttcgt gggccaaagt gagcacatcc ctcaggttac
     6781 cggtataatt cagcagaatg tatggatgaa gggaaggggt catgccggcg cagaaggccc
     6841 ccccgtactt tcccttgcgg ggaggggcat cgatccagcg tttttcgaag aattctcctg
     6901 caattcgacc gaagagagga tggaaccttt ggaaagattg caggagaagg tttctcgctt
     6961 cgtcgaaggg aatctttttg gggctggagg gcagcggagc ataaaggtca aagttcgcca
     7021 acttcggtag gcccagaaga caggccttga cctggaagta ctcccgggcc agaggatagt
     7081 gggcctccgt cacttccatc attaattcaa cggcttcggg gcgaatctcg ttctccagat
     7141 gaatccggtg cataggcccc tggtacccgc gaaggtcgtc ttctacctgg gcatcgagga
     7201 tgagggcgtt gaaaatggag cccaggacta aatgattctc cccatgctgc tttagaaata
     7261 tccggaaagc cttctcccgc aggctacgat cggaggaata aagaagggag agcatctggc
     7321 ttccggtgag ctccttctcc tccccctccg cttcgaggcg gaaggagaag gccccggtga
     7381 attcatcgaa gagggtagta aaagccgacc ttccggtcag gttcttccgg ctgaaaatct
     7441 cctcttcctt ttccccccgg gtaaacggcc ggaagcgccg aagaaagctc agggcgtgtt
     7501 ggtaggcttt caggggggga tgggacagga attcctggag tttttccggg gggaggccaa
     7561 tcagggcaag gcggaagaaa aggagctgat tttccagttc attccactgc tccttggctt
     7621 tctgcaggag ggttttgtat tcgttgttct gggaatcctg ggaaaaaaga aggctggcat
     7681 aaagaaaggg ctttatccct tcttcctgga tggattcgta ttctttcaaa gcttggaaaa
     7741 aagcggcagg ggagaggaga gaaagatcac cccccttata ggaagcctga agccgcctgg
     7801 cccgttccgc ccctcgattc aggtcgaccg cccaacttgg ctctctggga gtttggtaga
     7861 gatcggtcaa gtcccaggtg aacgcttgac cttccatgtc agggtccatg gtatcctatc
     7921 tttctattcc ttcgaaaagg agagaatccc ccacgagagg ccacctcgag cttgcgcccc
     7981 gggggataaa ttgccgaaga aacaggaccc gggaggcgcc ccccggacca gggttatgac
     8041 tgagggagaa aaaagaaagg ggagcaacta ttcagggatt cgaggagtga aaatcgccct
     8101 gaaaagaatc agacaggttt gggtccacca gaatcttttg cctctttttt catttcctct
     8161 ggcggttttt cggaggttcc ctcctcgatg gatttcactc ctcccttgaa gttcttaatg
     8221 gctttgccca gacctgaccc gatttccgga agcctcttgg ccccgaagat caggaaaacg
     8281 atggccagaa tgacaagcag ctcttgagtt cccaatccaa acaaattcct cacctcctta
     8341 aaacttcttc catttagtat attcttcctc ataggtttta gcaagggcaa aagccttttg
     8401 ccatactttt gccaatcatt gcgtgggggc ccccgtgggg ggtgaacgtc aatcgaaaaa
     8461 atgggaaacg aactcttcga aatcccaggc aatgaatccc ttggtcattt tggccacccc
     8521 cttgaaatag ggggagtcgg cttcctgaaa aagtcggtcg cacaaagaag gaacccagtg
     8581 attcagatgg tcctggaaaa acacctgctg caacttgagc caagggaaaa gctcttccat
     8641 ttttggggct tccgcagcct tctggcagag gtaaaagaca aactccagtt cggccccgat
     8701 atggtcatcc ggaaaagaac gatcgggatg gacaaccagc cccgcttcca tatatttttt
     8761 tcgcaccgcc agagtttcct cctgcatcat cagaccgctc tgggagcgat atacggattc
     8821 ataaaggggt acggccgaag gcccgacgaa gaggcgggtg aattccagga aaaggtcggg
     8881 caccagctgc ggcacccggg gggagaagat gattccgccc agcaaatgca ctccctcatc
     8941 caactccgga tggtagccgg caaggttttt caagacattc tcttcctgga gcgcctggaa
     9001 tagagcctcc gtgggttcct gaaggaagaa acagcggagc agatcataaa cgctctgccg
     9061 ggccttcatc agttcctgga attcatccat ttttaaactc attggggatt tgatatttca
     9121 gatcgcagat cgaataaatg gaaaagaatc gatctgggat cataaatcga aggtcccttt
     9181 cactcttccc ggattcgaat ctccacgttc ttgggcgatt cacaacccct aagagcggag
     9241 accattcccc gcaccgttgc gatgatcgat ttttggacga aaggcgataa agaaatatct
     9301 ttgccttcca ccttcagctg aaccgggggg gactttttcc ccaccaagaa tttttcttcg
     9361 atcaggtcga caatcccacg ggcatcgtcc aggtcgaaac agggtacgcc gatgtcaaag
     9421 ggctgattgg aggcgatggc cacgagattg tcttcccggg tgcaaagcag ctccctgcgc
     9481 ctctccttgc gaaagacttc tatcttgggc tgggagtttc tcttgaaacc ctccgagagg
     9541 atgatgtcca catcctggat gtacttgtcc cgcagctcgg ccagctcggc atcatggtcc
     9601 acatcccgga ttaaagccag tttttggggg gaggagatga ccaccgtatg agctccggct
     9661 tgcttatgcc gccagctgtc tttcccctcc cggtctacgt cgaagccatg aacatcgtgc
     9721 ttgatggtgg caactcgata gccccggagg tttagctcgg ggaccagctt ttcgatgagg
     9781 gtggtcttgc cgctgtcgga ggttccaact atggatacga tggggatcat gaaaatatca
     9841 ggtttcgagt ttcgggtttc aggtcaaaaa caatacctat cagctatgag caattctacg
     9901 cggttagcta tttctttttc aggacttcgg gattgatcgg gtattgggga ggttccccgc
     9961 gcaggacccg aactacttct tccaccacgg caatccccac gcgatagagg ctttcttcgg
    10021 tggtccccgc gtagtgaggg gttcccagga cctgggggtg gttcagaaga gggtctttgg
    10081 ggtcaggggg ttcaatctcc caaacatcca gggccgcggc agcgataggc ccggagttga
    10141 gagcttccag gagggctttc tcattcacga ttccgccccg ggcagtattg atcacgtagg
    10201 tccctttttt catcatggcc aattcccttt taccgaccat tcccttggtt tcctgggtca
    10261 gggggcaatg gaccgtcaga aagtcgatct tggggagcag gttttccagg cgctccacct
    10321 tctccacccc gaaggtcttc atgacttctg cggatacata ggggtcgtaa cctcttacct
    10381 tcatgtcaaa agcggcaatg cacttccggg cgacggtgga accgatgcgg cccgttccga
    10441 tgatccccag cgacttgcct tccaaatcaa tgcccgtcag gttgtactgt ttcaagaggg
    10501 cgctgaagat cccgacatcc cccttaggca tattggccat ggctttctgg gcctgaggaa
    10561 ttctccgggc cagggccagc atgaaagaca gggccagctc ggccacggac tcccggttcg
    10621 caccgggagt attggttacc aggatccccc tgcgcgtagc ttccgccagg tccaccgaat
    10681 ccagccccac tccatggcgg gcgatgattt tcatcttggg agccaggtcc atttcctcgg
    10741 ctcccacata aactttgcgg gctaccatgc cgtctacctc tttcagcgct tccaggtaag
    10801 cctgccggga atggtctttc gggcgaacga cctgggcgtg tttctccaaa agggctaccg
    10861 catcaggatg gatgacctct cccagataaa ccttcatttt ccctcctcaa cggatttcaa
    10921 aatagctttt gtcccccctc ccattctcct catgaatgga aaagggtcgt gtcgcctatc
    10981 tatcatccct aaaagggggc tgtcaatcag acaggcaacc cccgggggct ttctcatggc
    11041 aagaaaaaaa ggacccccgg ttctaaaacg agggtccaaa agtgtagagc aaaaaggaaa
    11101 aacctatgcc cgcaggcagg cggagagcag attctgggat ttgatcttct ccaaccatac
    11161 ctcgtattgt ccctcgtctc ccagaatctg attgacgagt tcctcctggt aatgcccctc
    11221 tttcatgcgg accttgatat gcttcttaaa tctctcatga agggtgtaat gaatgatgat
    11281 cccgaccgcc gcactgtaag gacaaagggg ccctcccaca gtgtacgaga cccggatttc
    11341 ttcttcctga agctcgatat cgtcctcccg gacgaaaccc atttccacaa tgctgcggtc
    11401 cttgaggtcg gggtcgagaa tgtgatccag caccctcaat atatcctgct tggtcacggg
    11461 aaaccctcct atgaattcaa ggggccataa aaatcggaaa gatttagtgc ccgtgttcgt
    11521 gctcgtccgg taattccttc ccacaccctg tacagaactc cgcctcgtcg ggattctcca
    11581 catcgcagaa gggacagtag catttcttgg gggtcgcctt gtaggcagtc ccttcctttt
    11641 tcattttaaa atcctcgatg gccttatgaa gagcatcggc tcccaggttg gagcagtgca
    11701 tcttctgttt gggaagaccc tccaacgctt cggccacctt tttgttggtg atctgggaag
    11761 cctcatcaag ggtcttgcct ttggccatct cgctgaccat gctggaaacg gcaatggcgg
    11821 ctccgcaacc gaaggtctgg aatttcacgt cttcgattac gttttccttg actttgatgt
    11881 aaaaggtcat catatctccg cagaccggat tccccacttc tccaacccca tccgcaccct
    11941 cgatgacccc cacattgcgg ggattcttga aatgatccat aaccttctcg gtgtacatgt
    12001 cttctttcct tccttttttc ggattttatg gtcctcaggt ttttttagcc atcgccttcc
    12061 gatacaccgg ggacatattc cgcagccttt ccacgatagg cggcaatacc tgcaggatgt
    12121 gatccacatc ctggggcttg ttttgcattc cgaaggagaa caatagggat ccttgggccg
    12181 tggcggcatc cacccccatg gcgagcatga catgggagac cttgagagaa cgggaaatac
    12241 aggcagagcc gctggaaata cggacccctt ccatgtccat gaagaggagc atggcttcgc
    12301 cttcgatgta ttcgacggc
//
LOCUS       DASPRR010000419         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1064866, whole genome shotgun sequence.
ACCESSION   DASPRR010000419 DASPRR010000000
VERSION     DASPRR010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064866"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VEL68_15270"
     CDS             complement(<1..162)
                     /locus_tag="VEL68_15270"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXZ37380.1"
                     /translation="MNFNHQREAKMDKIIITVATTGSRPTKEQNPNVPISPEEIANSA
                     YECFNEGASI"
     gene            complement(194..1294)
                     /locus_tag="VEL68_15275"
     CDS             complement(194..1294)
                     /locus_tag="VEL68_15275"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ37381.1"
                     /translation="MKISRVEAIPFHIPFDPDFHLKFAYRTSGAADHVLIRIHTDEGL
                     VGIAEAPARPEIYGETQKSIVSLVDQYLGPYLAGKDPFLLEEINALLDRIPYNYCAKG
                     GVDIALHDLLGKSLNLPVYKVLGGKSRDRVPLSWMVGINSPEKMARECERFMGRGIQA
                     FKIKAGVDFAEDRERFLAIRKAVGERALLYIDANQGFKSLKKTVAFLRELQEHGLAFV
                     EEPFPVRNRKDRKEAARLLSIPLMGDESCFTPWDVAQELDLGALGIVLVKVARTGFFK
                     TRKILHLCQQAGIPCMVGSQGDSSIGAAASLHAAVAFSNILFPGEVSYHLRMEGDLLK
                     DPPEFKDGELTVPDRPGLGIEIDEKALEKFRV"
     gene            complement(1409..2560)
                     /gene="iadA"
                     /locus_tag="VEL68_15280"
     CDS             complement(1409..2560)
                     /gene="iadA"
                     /locus_tag="VEL68_15280"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HXZ37382.1"
                     /translation="MMKAIKNGRVYSPGDLGQKDILITGDTILAVADRLSTESLPFPV
                     EVYDAGGKMILPGIIDPHVHFIGGGGSSGLNSRTKEIAVEQIIEAGVTSAVGVLGFDR
                     ASRTLKALLLKTRALEDLGLTAFMLTGSYSLPSQTLTGSVEDDLILIDKVIGVKLALG
                     ETLANFPDIRDIRNLLAECLRGGHLGGKAGFLQIHMGVQGKVWKKPFMEILREMSIPF
                     SQVVFTHVNRSPDTLDEFLEYIQAGGGVDLTASYTPTERPGSLTVGKALQRLIRAGVP
                     LDRVTLSSDSNATRMLPDKRLKYLPVQTLFQVVRDLWLSEEIPRPQLVRLVTENPARS
                     LGLGRIKGLVEPGKHADLIILKEPFELDGVILKGKWGRRDGQTLIRDPF"
     gene            complement(2677..3579)
                     /locus_tag="VEL68_15285"
     CDS             complement(2677..3579)
                     /locus_tag="VEL68_15285"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ37383.1"
                     /translation="MGSIKVGVIGTGRMGNPFALNLLKAGHQVRVFDIDPKATQNLVA
                     QGAVLSASPAEMAPQAEYLLTSLPRNEIVEQTIIGPQGLLPRLNRGSIVIDLSTTLPR
                     TTKAIAAALARKGVDFLDAPVSGHAVGAKNATLTIMVGGTKEAFLRAREPIFEKLGKN
                     IFHAGPTGAGQALKLVNNLLYNLNRLAMCEGLVLGAKAGIDPEIMCQAISLSTGGSYV
                     IRNLPPDILEGNFEGRESSLNLACKTLKLITDFADELNVNLLLGNLAKQLYNFMRFQG
                     HGEETPSTVIQFYESAAGVKVRKS"
BASE COUNT          787 a         1185 c         1083 g          855 t
ORIGIN      
        1 gatgctggct ccttcgttga agcactcata cgcggagttg gcgatctctt cggggctgat
       61 tggaacattg gggttctgct ccttcgtcgg cctcgaaccg gtggtcgcga cggtgatgat
      121 gattttatcc attttggctt ccctctgatg attgaaattc attccggtcg aggcccgatc
      181 cgaggaccgg gccttacacc cggaatttct ccagcgcctt ttcgtcgatc tcgattccca
      241 gtcctggccg atcgggtaca gtcagctctc catctttgaa ttccggcggg tcctttaaca
      301 gatccccctc catccgcaaa tgataggaaa cctcgcccgg gaagaggatg tttgaaaaag
      361 ccacggccgc atggagacta gccgccgcgc cgatgctcga atctccctgg cttccaacca
      421 tgcaggggat cccggcctgc tggcagagat ggaggatctt gcgggttttg aagaagcccg
      481 tccgggccac cttcaccaaa acaatcccca gagcccccag gtccagctcc tgggccacgt
      541 cccacggggt gaagcagctc tcatccccca tgagcggaat ggacagcagg cgggcggctt
      601 ccttccggtc cttccggttg cgcacgggga agggctcctc cacaaaagcc aacccatgct
      661 cttgcagttc acgcaggaag gccaccgtct tcttcaagga cttgaacccc tgattggcgt
      721 cgatgtagag gagggccctt tcccccaccg ctttccggat tgccaggaac ctctcccggt
      781 cctccgcgaa atccaccccg gccttgatct tgaaggcctg aatccccctc cccatgaatc
      841 tttcgcattc ccgggccatc ttttccgggg aattgattcc caccatccag gacaggggga
      901 cccgatcccg gctcttcccg cccaaaacct tgtagaccgg caggttcagg gatttcccca
      961 aaagatcgtg tagggcgata tccacgccgc ccttggcgca gtaattgtaa gggatgcggt
     1021 cgagcagggc attgatctct tccagaagga aagggtcttt gcccgccagg tagggcccca
     1081 ggtactggtc gaccagagac acgatggatt tctgcgtctc cccataaatc tccggccggg
     1141 ccggagcttc ggcgatcccc accagcccct catcggtgtg aatccggatt aagacatggt
     1201 cggccgctcc ggaagtgcgg taggcgaatt tcaggtgaaa atcgggatcg aagggaatgt
     1261 gaaagggaat cgcttctacc ctggagatct tcatgagtcc tccgtgaaaa gatcgtgaaa
     1321 gtgaaagtgt gagcgaaagg cggggaaaag aagaccatcc accttcacct gcccttcttg
     1381 caccttcact tgcaccttca ctgtcttatt aaaatggatc ccggatcagg gtctgcccgt
     1441 ctctgcggcc ccatttcccc ttcaggatga cgccgtccag ctcgaagggc tccttcagga
     1501 tgatcaggtc cgcatgtttc ccgggctcga caaggccttt gatccgcccc agccccaggg
     1561 accttgccgg attctccgta accaggcgga cgagctgggg tcggggaatc tcctcgctca
     1621 gccacaaatc ccggacgact tggaaaaggg tctgaacggg aaggtatttt agcctcttgt
     1681 ccggaagcat ccgggtggcg ttgctgtcgg aactcagggt cacccggtcc aagggcactc
     1741 ctgctcgaat caatctctgg agcgctttcc ccaccgtcag gctccccggg cgttccgtgg
     1801 gtgtgtagga agcagtcaag tccacccccc ctccggcctg aatatactcc agaaattcat
     1861 ccaaggtatc cggagaccgg ttcacgtgag taaaaaccac ctgggaaaag gggatgctca
     1921 tctcccggag gatttccata aagggctttt tccaaacctt tccctggacc cccatatgaa
     1981 tctggaggaa tccggccttc cccccaaggt gcccgccccg aagacattcg gccagtagat
     2041 tccggatgtc ccggatatcg gggaagttgg caagcgtttc gccgagagcc agcttaaccc
     2101 cgatgacctt gtcaatgaga atcaggtcat cctccaccga acccgtgagg gtctgggagg
     2161 gcaggctgta ggaccccgtc agcatgaagg cagtcaatcc caggtcctcc agggctctgg
     2221 tcttgagcag cagggccttc agcgtccggg aggctcggtc gaaacccaga acccccactg
     2281 cgctggtcac tccggcttcg atgatctgct caacggcaat ttccttggtc cgtgagttaa
     2341 gtccggaaga accccctccg ccgatgaagt gcacatgggg gtcgatgatc cccggaagga
     2401 tcatcttgcc ccccgcatcg tagacttcta ccgggaaagg aagagattcg gtcgaaaggc
     2461 ggtccgccac cgcgagaatg gtatccccgg tgatgagaat atccttctgc cccaggtcgc
     2521 cgggagaata gaccctccca ttttttatgg ctttcatcat gattcctcca gaccttggcg
     2581 gcccgatcgg ggatcgggcc ctacgattca aagaaccgaa tgtagggcgc gttccccgaa
     2641 cgcgccgatg aaatgaacat gggtcttcct tccccttcag gatttgcgga ctttgactcc
     2701 cgcagcggat tcgtagaact ggatgacggt cgaaggagtt tcctctccat ggccctggaa
     2761 gcgcatgaag ttgtagagct gcttggccag gttcccgagg aggaggttca cgttcaattc
     2821 atccgcaaag tccgtgatga gcttgagcgt tttgcaggcc aggttgaggc tgctctcccg
     2881 gccctcgaaa tttccttcga gaatatctgg gggaagattg cggatgacgt agctcccgcc
     2941 cgtgctgaga gaaatggcct ggcacatgat ttccgggtca atccccgcct tggctcccaa
     3001 gaccaaccct tcgcacatgg ccaggcggtt caggttgtag aggaggttgt tcaccagctt
     3061 cagggcctgc cctgccccgg tgggcccggc gtggaaaata ttctttccca gtttctcgaa
     3121 gatgggctcc cgggcccgga ggaaggcttc cttcgtcccg cccaccatga tcgtgagcgt
     3181 agcgttcttg gcccccaccg catgtccgct caccggggca tccagaaaat cgaccccctt
     3241 ccgggccagc gctgcggcga tggccttggt ggttctgggc aacgtcgtgc tcaggtcgat
     3301 gacgatcgac ccccggttca gcctcggcaa aagcccctgc ggcccgatga tcgtctgttc
     3361 cacgatttca ttcctgggaa gggaggtgag gagatactcg gcttgagggg ccatttccgc
     3421 cggcgaggcg ctcaagaccg ctccctgagc gaccaaattc tgagtagcct tcgggtcgat
     3481 gtcaaacacc cggacctgat gacccgcctt gaggagattc aaggcaaagg gattgcccat
     3541 ccgtcctgtt ccgatcacgc ccacctttat tgaacccatt ttcctctccc ttgtccctta
     3601 ttcaagcttc gcaaaagttc taaccaaagg catttgaaat tcttctttag agtcctcccc
     3661 ttttggagac tgtgtcgtaa tcccccggtc gcccttcgac aggctcaggg cgaacggcta
     3721 agtagctgaa tattaatgcg ctcataatcc gttcgtggtg agcttgtcga accatgaacg
     3781 gattacgaca cagtctcttg ataggggggg taagggtggg ggtgaatcgg gaatgcatcc
     3841 ccctcccctt gatcccctcc cgcgagggga ggggaaaatt gtagaggcat gattcatcgt
     3901 gcctctacgg
//
LOCUS       DASPRR010000420         7028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1065275, whole genome shotgun sequence.
ACCESSION   DASPRR010000420 DASPRR010000000
VERSION     DASPRR010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065275"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(563..2131)
                     /locus_tag="VEL68_15290"
                     /pseudo
     CDS             complement(563..2131)
                     /locus_tag="VEL68_15290"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2271..3029)
                     /locus_tag="VEL68_15295"
     CDS             complement(2271..3029)
                     /locus_tag="VEL68_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37384.1"
                     /translation="MIELEGLTKKFGSFYAVRDLSLKVSPGEILGFLGPNGAGKTTTI
                     RMMAGLMKPTSGRILLDGKDVFGRPDEAKAILGYIPDRPYLYEKLTGGEFLEFIAGLH
                     QLGKEDGWEKRSQGLLEYFDLLPWRRELIESYSHGMRQRLIIIAALLHRPKVLIVDEP
                     VVGLDPQGVRLVKSLFRDLVDDGMTIFLSTHILGIAEELCPRIGIIHEGQLLALGSMS
                     DLREQAQSRDERLEPIFLKLTQEEEPRTESFGVP"
     gene            complement(3026..4474)
                     /locus_tag="VEL68_15300"
     CDS             complement(3026..4474)
                     /locus_tag="VEL68_15300"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXZ37385.1"
                     /translation="MVWGALMGLLVERNFFQPQALKVTPALARDGLRTGEEWWGVYWK
                     EEKIGFAVTEQDLREEKIRVRERLWLKMAILGVPQSIEQTLDYTLTERLTLESFDFNL
                     RSGLFPFRISGRLQDLPQDSRKKLLLKVRTGGKEQEQQVFLAEAPFILGQTKLHFLTQ
                     GLEKGKKYRIPVFDPTTLTSAEMIAEVEEVESMTIKGEKRKLYRVRQECRGLDVKSWI
                     DQNGQTWKEESPAGLVLVRESPAEARHQHWSPGKMADLLSLTAVPSDREIPNPRSLAF
                     LRVKLQSSGLGGLKIEGDRQTRSGNEVTIIREKIPPDSSANQPLPDPEKGESLNPTPF
                     IQSDHPEIRRLALGIAGGAQEQTEQVKRLTAWVYREVRKQPVMSVPSALDVLQQRVGD
                     CNEHAVLFVALARALGIPARQQAGVIYQEGKFFYHAWAQVYLGAWVSVDPILNQVPAD
                     AAHIRLVEGDLDRQAELVRVIGRLKVEVKEFH"
     gene            complement(4663..5370)
                     /locus_tag="VEL68_15305"
     CDS             complement(4663..5370)
                     /locus_tag="VEL68_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXZ37386.1"
                     /translation="MEPPPRKPRPIRLALFDLDGTLTRERSAWEYLHRRLGVWEGQAE
                     KYQEAFLRGEIDYPRFCQLDAAVWKGMKVYALEGMLKEVPLYEGIEDLVAYLKSRDIK
                     LGIISSGLSLLAEWIKERYGFDFSVANELGVTDGVLDGTIRIHVHHDQKGRWVKEAKR
                     QFHAKKEEVLALGDSSGDIVMFQQAGLSIAFNSRSSRLNEIATLSIYSSDLRDLIPPL
                     ISYQSSARGILEARKGG"
     gene            complement(5458..5940)
                     /locus_tag="VEL68_15310"
     CDS             complement(5458..5940)
                     /locus_tag="VEL68_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HXZ37387.1"
                     /translation="MVEMKVHGLFFDSETNQSVVVLKEETTGRTLPIWVGLFEANAIT
                     MGIEHTWTPRPMTHDLLKNVIEGMNAVVREIAVNDLRSNTFYALISLEVGGKLVKIDS
                     RPSDAIALALRTNSPIFVEEKVLNSAGHSEGKAKKDVSDKWIDDLFDKLKPEDFKTEM
                     "
     gene            complement(6029..6769)
                     /locus_tag="VEL68_15315"
     CDS             complement(6029..6769)
                     /locus_tag="VEL68_15315"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HXZ37388.1"
                     /translation="MKEDLIEKTARVISRSKKVVAFTGAGISVESGIPDFRGAGGLWE
                     KFDPAEYATIEAFHANPRKVWAMLKEMGDLLESSRPNPAHRALARLEEGGYLRSVITQ
                     NIDHLHQDAGSKRVIEYHGSHKNLVCMSCGQHYRKEEVKLESLPPLCSCSGVLKPTVV
                     FFGEPIPWGAHLQAMEEARDCEVMLVIGTSAVVSPACEIPVLAKKGGAALVEVNLEET
                     QLTGYVSDWILKGSASRMMQAVMDQMGG"
BASE COUNT         1630 a         1916 c         1913 g         1569 t
ORIGIN      
        1 tccgggtact tgtgatgaac gacccagtcc cccactttca gatatagaca tcgcctttct
       61 tccataattg gcttatatca aactcccacc tattccgcaa gcggaaaaat caggccattt
      121 catgggattc gcttgccggg cccaaagacc ctttcccaga aaacccttca gcctcccttt
      181 ttcaaatgga agagaacttt gtcacaaggc tttggcaaga gaccaaatat tttctttttg
      241 gaaaagggaa ttttatattt ttatttcttt ttaaaaaatg caccctttct ttcaaattct
      301 ttataaacta gtttaagttt ccgaaattct ttcccgcatt tccagcaggt caagggaatt
      361 tcctgagcct gacgcaagcg gcctcgggtg gcaaaattct gggttcggca gtaaggacag
      421 ggaaaaactc tgctcataaa tcgttgctgg ttgctcgtta ctggctggtt gttgctggtt
      481 gctggttata ggttgccaaa tgccgtcttg aaaacgcatt ctttaaccac caaccagaac
      541 ccaggaacca gcaacgtttt tttcaaaaat ccatctcctg aagatttctc aggccaacct
      601 tcatgggaag gagagtggcc gttccgatca ggccagcgat cccggaaaag gccccgcaaa
      661 ccccgatcca ctgaaacacc gagaggctgc tttgttggaa tccggccatg aaaagaatgt
      721 aaaccggcca ggcttccaaa gccactaaaa ccccgataaa gatcatactc agaatcatga
      781 aaatggcccc cccgaatccc caagccatac gggccgcatt ttcaagcctg aacctggggt
      841 agatcgcccc caatcccaca gccaaaccca cgatcccaaa agtcatgaag aaaatcgtca
      901 cggcggaaag gatcatcaag aatggagttg ccttcaaaag ccagttggag aggaatatga
      961 gggcttccgc caggaggaga agcgggacta ggccggtcca gaatttgctc cacaaaaagg
     1021 tccgcagggg gaccggggag gagcgaatga tccagaaaga gaacccttcc tgactgaccg
     1081 ccgggaagat gaatcggccg gctacagcgg acaggacgaa accggccaat cccaggttca
     1141 ggaaggagac taggttctgg aggaaaaagc tgggcatagg agttcggtca agcggtaaga
     1201 cactgaaatt gtagagatag accaccacca aagccatgag gagaatgagt tgggaccatt
     1261 gggaggtatc ccggaaaaag gtcttccaat cttttaggac cagggctcgg gggaaggggc
     1321 gaaggaaccg gctgagccct tgcagaagcc ggttgaagag aggaaggcgc accaggtaga
     1381 ccctacgggc ttcctgggat ttcgtccagc cggaaatgaa gagggcccgg gaaacccaac
     1441 ttcccattac cactaaggca cctgccgttg accagagaca ggcaaggtaa aaccatgcct
     1501 gcaccggagc cggttgcagg aagggaatca tcgattcagc aaaccaaaag ctgggggaaa
     1561 agggccacga cggagcgttt aaagcggtca tatattccac caggctggcg aaggaatcgg
     1621 ggttgaccaa tctttcgggc tggagaaacc ggaacaagaa atagatgccg atgaggagaa
     1681 agatggtcag gagaaataga atgtccttgg tcctccgggc aggaaagata ttgaccagga
     1741 acatggccaa gaggaccccc agagccgaag gaatgagaag gaatggaccc aggcctccca
     1801 cgaagacggc ataatatttc cagggagccc catacaccca accgtaggcc agaaatacgg
     1861 gcaaggcgaa gagaaggacc atccaggaac tgtaaaccag ggtttccgtg agccgcgcat
     1921 agtagatgtc atccagaggg acaggccggc tcaatatgag attcaggtct tccgagagga
     1981 aaaaagtgga aagagaggag atgaggttgc tgaaaaggag aatggagaaa aaggtcagga
     2041 gcatcatgga aagaagccgg tagttcaaaa ggtccccaaa aagctcgacg gaatggaagt
     2101 agaccaggac cttctggaat acaaggaagg tgaagagcca aacccaaggg ccaggacaac
     2161 caggaaggtc agcttcagcc gcgcccggat ggactgttca ccgagatgat ggctaaagga
     2221 gcggattttg ggaaagagaa gtgcgggcca tccctccatc gacatttccc ctatggaacc
     2281 ccgaaacttt ccgttcgggg ttcctcttcc tgggtcaatt tcaggaaaat gggttccaga
     2341 cgctcatccc ggctctgggc ctgttcccgg aggtccgaca tggaccccag agcgagcagt
     2401 tgaccttcat ggatgatccc gattcgtggg cacagctctt cggcgatccc caggatatga
     2461 gtggagagaa agatggtcat tccatcgtcc accaggtccc ggaagaggct ttttaccaag
     2521 cgaactccct gggggtccaa gccaaccacc ggctcatcca cgatcaagac ctttggacgg
     2581 tgaagcaggg ccgcgatgat gatgaggcgc tggcgcatcc catgggagta gctttcgatc
     2641 agttcccggc gccagggcag aaggtcgaag tattccagca gtccttggga acgtttttcc
     2701 caaccgtctt ccttccccag ctggtgaagg ccggcgatga attccaaaaa ttctcccccg
     2761 gtcagcttct cgtaaaggta cggccggtcg ggaatgtatc ccaggatggc tttggcctca
     2821 tcgggccttc cgaatacatc tttgccgtcc aggagaattc ttccggaagt gggcttcatc
     2881 agtccggcca tcatgcggat ggtggtggtc tttccggccc cgttgggccc gaggaatccc
     2941 agaatctcgc ccgggctgac tttcagcgaa aggtctcgca cggcgtagaa ggagccgaat
     3001 tttttggtca gaccttccag ttcgatcaat gaaactcctt tacttccact ttgagtcgcc
     3061 cgatgactct taccagctcc gcctgccggt ccaagtctcc ttccaccagg cggatatggg
     3121 ccgcatcggc aggaacctgg ttgaggatgg ggtccacgga aacccaggcc cctagatata
     3181 cctgggccca ggcatggtag aaaaattttc cctcctggta gataaccccc gcttgctgcc
     3241 gggccggaat tcccagggct cgggctaaag ccacaaacag gacggcgtgt tcgttgcagt
     3301 ccccgactcg ctgctgaagg acatccaggg cgctggggac actcatcacc ggctgtttgc
     3361 gaacctcccg atacacccaa gcggtgagcc tcttcacttg ctcggtctgt tcttgagccc
     3421 ccccggctat gcccagggcc agtcgccgaa tctccggatg atcactctgg atgaaaggag
     3481 tcggattcag gctctctcct ttctcagggt ccggaagggg ctggttcgcc gaggaatcag
     3541 ggggaatttt ttcccggatt atggtcacct catttccgga tcgggtttga cggtcccctt
     3601 caatcttcag cccgccgagg ccgctagact ggagtttgac ccgtaaaaaa gcaagggatc
     3661 ggggattggg aatttcccgg tcgctgggaa cggctgtgag ggacaaaagg tcggccatct
     3721 ttcccgggct ccagtgttga tggcgcgcct cggccgggct ttcccggacc aggacaagtc
     3781 cggcggggct ttcttccttc caggtttgac cattctgatc tatccaggac ttcacgtcca
     3841 gcccccgaca ttcctgccgc acccggtata gtttccgttt ttcgcctttg atcgtcatgc
     3901 tctccacctc ttccacctcg gcgatcatct ccgccgaggt cagggtggtt ggatcgaaga
     3961 ccgggatgcg gtacttcttc cccttctcca gcccctgggt caaaaaatga agcttggtct
     4021 gtcccaggat gaaaggggct tcggccagaa acacctgctg ttcctgctcc tttccgcccg
     4081 ttcggacctt cagaagaagc tttttcctgg agtcctgcgg gaggtcttgc aggcgaccgg
     4141 aaattcggaa ggggaagaga cccgaccgga ggttgaaatc gaaagattca agagtgagcc
     4201 gttcggtcaa ggtgtaatcc agagtctgct cgatgctctg aggcactccc agaatagcca
     4261 ttttaagcca gagacgctcc cggactcgaa tcttttcttc ccgcaggtcc tgctccgtga
     4321 cggcaaatcc gatcttctct tccttccagt agactcccca ccattcctca ccggttctca
     4381 gcccgtcgcg ggctagagcc ggggtgactt ttagggcttg gggttggaag aaattccgtt
     4441 ccacgagcag ccccatcaaa gccccccaga ccattacaat tcctactttc aggagtgttc
     4501 tggtccagcg ggggagtctt ttggaaggaa agggccgctt tttctccata cctgttttca
     4561 tcggaaagga cgatctaaac ttgaaggagg ctttctcgct tgtgaatcgc cggatgggtt
     4621 cggggacgtt cggccttcgt cctcaaggac gagccaaaaa tctcagcccc ctttccgtgc
     4681 ctctagaatc ccccgggctg agctctgata agagattagc ggagggatca ggtcccttaa
     4741 gtcggagctg tagatggaaa gggtggctat ctcattcaaa cgagaagaac gagagttgaa
     4801 ggcaatggaa aggccggcct gttgaaacat aacgatatct cccgagctgt cgcccagcgc
     4861 caggacttct tccttcttcg cgtggaactg tcgtttggct tctttgaccc accttccttt
     4921 ctggtcgtgg tgaacatgaa tccggatggt tccgtcgagg actccgtcgg tcaccccgag
     4981 ctcgttggcc acggaaaagt cgaaaccgta tctctctttg atccactccg cgagcaggga
     5041 gaggccggaa gagatgatcc ccagcttgat gtctcgagac ttgagatagg caaccagatc
     5101 ttcgatccct tcatagagcg gcacttcttt cagcatccct tccagagcat agactttcat
     5161 tcccttccat acggcggcat ccaattggca gaagcggggg taatcgattt ctccccgaag
     5221 aaaagcttcc tggtactttt ccgcctggcc ctcccatacc cccaggcgtc ggtgaagata
     5281 ttcccaggcg cttctttccc gggtcagggt tccgtccagg tcgaacaagg ccaatcggat
     5341 gggtcggggt tttcgagggg gaggttccaa agcttctgag gaccttccgg aggagaaaat
     5401 cggtctcacg ggctgaaggg ctcgggttct ctgggtttgg tgtggagcag ttcttcccta
     5461 catttcggtc ttgaaatctt cgggttttag cttgtcgaag aggtcatcga tccatttgtc
     5521 ggaaacgtcc tttttggctt tgccttcgga gtgtccggct gagttgagca ccttctcttc
     5581 cacgaaaata ggcgagttgg ttctcaacgc cagggcgatg gcgtcgctgg gtcgggaatc
     5641 gattttgacc aatttccccc ccacttccaa agagatgagg gcgtagaaag tgttggaccg
     5701 caaatcgttt accgcaatct cgcggactac cgcgttcatt ccttcgatga cattcttcag
     5761 gagatcatgg gtcatggggc ggggagtcca ggtatgttcg attcccatgg taatggcgtt
     5821 ggcctcgaag agccccaccc agatgggcag ggttcggccc gtcgtttctt ccttgagaac
     5881 gacgacggac tgattggtct ccgagtcgaa aaacaatccg tgcactttca tttccaccat
     5941 ggtctctccc ccttttcctg aatttccctc gttttattat agtccggcgg gggataaaaa
     6001 gccaccgcgt ttttcggccg gggtcaattc aaccccccat ctgatccatg accgcctgca
     6061 tcattcggct ggcggagccc ttcagaatcc aatccgaaac gtagccggtc agctgggtct
     6121 cttccaaatt cacttccacg agagcagcgc cgcccttctt ggccaggacc gggatttcgc
     6181 aggccgggga tacaacggcc gaggtaccga tcaccagcat gacttcacaa tccctggctt
     6241 cctccattgc ctgcagatga gcgccccagg gaatgggctc tccgaagaat actacggttg
     6301 gtttcaacac cccggaacag ctgcagagag gcggaaggga ttcgagcttc acctcctcct
     6361 ttcgatagtg ctggccgcag ctcatgcaaa ccaggttctt atggctcccg tggtattcaa
     6421 tgacccgctt gcttcccgca tcttggtgaa gatggtcgat gttctgagtg atgacggaac
     6481 gaagataccc cccttcttcc agtctggcca aggctcgatg ggccgggttg ggacgggaac
     6541 tctccagcaa atcccccatc tctttgagca tggcccaaac ttttctaggg ttggcatgaa
     6601 aggcttcgat ggtggcgtac tcggcggggt cgaatttttc ccagaggcct cctgcgcccc
     6661 ggaagtccgg aattccgctc tccacggaaa tgccagctcc ggtaaaggcg accaccttct
     6721 tcgaccggga gatgacccgg gcggtctttt cgatcaggtc ttctttcaag agccccccct
     6781 tcaaatgcgg aatggagaat gtggaataaa tatcgttttt ttcttcccct accgggggaa
     6841 ggtgcgatca ttctatcaga gcggatgatt ttagcaaggg cattcaaaat tcaaaatctt
     6901 gcccaccccc agtttttcat gcctgttggg gttcagggtg gaagactttg gatcatcagg
     6961 ctcggcgggt gcttatcctt ccatgaaact cctccccatg ttccgcaggc agaggcctga
     7021 gaggagat
//
LOCUS       DASPRR010000421         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1392116, whole genome shotgun sequence.
ACCESSION   DASPRR010000421 DASPRR010000000
VERSION     DASPRR010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392116"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VEL68_15320"
     CDS             complement(<1..367)
                     /locus_tag="VEL68_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37389.1"
                     /translation="MEQSVRDEILAKLRAAPKAEVPTRPILPPYKTLSMDREQLVAEF
                     TARLAEEMVVVHRVKDLRAAEDKLSEVAVEEGLKKVMTSTDDVISQLDLPAWGKKNDI
                     QVMTPRDFADRYSYRDAVFD"
     gene            complement(373..1812)
                     /locus_tag="VEL68_15325"
     CDS             complement(373..1812)
                     /locus_tag="VEL68_15325"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HXZ37390.1"
                     /translation="MEIKTEQFTELAKKELKNDRSRLFLKLIPPVMKGKREAGLASLP
                     DAPAAMACGAAIRAEALSRLPELLEEFEKNATANGMKVVWTRDDKEANEFIKNLAKEK
                     GIQYVTKGKSMVTEETALNELLEKNGIEVWETDLGEFIAQQLHLPPFHIVGPAINVPV
                     EEVRDLFMRKIGLKDPTLDPVQLGYAARLFLRDKFHHVQMGITGVNMAVAETGTIINV
                     ENEGNIRFTKASPPVQVSVMSLEKVVATMEDALHMIRLVCRNCTGQRLSVYVSMDSRP
                     KKKDEIDGPEEIYIVIVDNGRSKIYQDVKARDALRCIRCGACLVACPVWRQIGGYAYG
                     WAYSGPMGQVLNPMLLGLERTQELYRASTLCGACKSFCPVGIDHPSLFLYYRAKDVLG
                     DPHFKSVDRPWMERQFFKMWSWAASTPWAWNMGVRMARPYINKDAKDGILSKAKGPFQ
                     GWFRSRDIPAMAGFTFHDRWKEKLRNKGE"
     gene            complement(1802..2551)
                     /locus_tag="VEL68_15330"
     CDS             complement(1802..2551)
                     /locus_tag="VEL68_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ37391.1"
                     /translation="MMKEVSLFIPCTVDLFAPRIGEAVVHVLRRVGVDPIYHEEQTCC
                     GQPAMNAGYREEAKKVAKHFISVFEKDPVIVSPAGSCVYMVKDHYPEILKDEPDWFRR
                     AKELAPRVYEFSQYLVDVLKVEDVGASFEGKVTYHESCHILRGLGISEQPKKLIQAVK
                     GVQLAPLDNADSCCGFGGEFANDFPDISESMVKEKVENFLASGADLLLLNEPGCFLNI
                     GGYLNRNHPGKKIMHLASFLATNGREAEYGN"
BASE COUNT          593 a          805 c          760 g          655 t
ORIGIN      
        1 gatcaaagac ggcgtcccgg taggagtaac gatccgcgaa atcgcgcggg gtcatcacct
       61 ggatgtcatt cttctttccc caagctggca ggtcaagttg ggaaatgaca tcgtcggtgg
      121 aagtcatgac tttcttcaac ccctcttcca cggcaacttc actgagctta tcctcggctg
      181 cccgcaggtc tttgacccgg tgaaccacga ccatttcttc ggccaaacgg gcggtgaatt
      241 cggcaaccag ctgttcccga tccatggaga gggttttgta agggggcaga atcgggcgag
      301 ttgggacttc cgccttcggc gccgctctca attttgccag tatttcatcc cgcacacttt
      361 gttccattcg tcctattccc ccttgtttct tagtttttcc ttccatcgat catgaaaggt
      421 aaacccagcc atggccggga tatcccggct gcggaaccat ccttgaaaag gccccttggc
      481 tttgctgagg attccgtctt tggcatcctt atttatatat ggccgggcca tgcgcacccc
      541 catgttccag gcccaggggg tgctggctgc ccaactccac attttgaaga actgcctttc
      601 catccagggt cggtcgacac tcttgaaatg ggggtccccc aggacatcct tggcgcggta
      661 atacaggaaa agactcggat ggtcgatccc tacggggcag aagctcttgc aagccccgca
      721 cagggtggaa gcccgataaa gttcctgagt tcgttccagt cctaggagca tcgggttcaa
      781 aacctggccc atggggccgg agtaagccca accgtaggca tagccgccga tctgtcgcca
      841 gacagggcag gctaccaggc aagcgccgca gcgaatacag cggagggcgt ctcgggcctt
      901 gacgtcctga tagattttgg agcggccgtt atcgacgatg acgatgtaga tctcttcggg
      961 gccgtcgatt tcgtccttct tcttgggcct gctgtccatg gatacgtaaa cggagagcct
     1021 ctggccggtg caatttcggc acacaagacg gatcatatgg agagcatcct ccatggtagc
     1081 aaccaccttc tccagactca tcaccgacac ctgaacggga ggagaggctt tggtgaagcg
     1141 gatattccct tcgttctcaa cgttgatgat cgtcccggtt tccgccacag ccatgttcac
     1201 cccggtgatc cccatctgaa catggtgaaa cttgtcccgc aggaagagac gggccgcgta
     1261 ccctagctgc accgggtcca gcgtagggtc tttcaggccg attttcctca tgaaaaggtc
     1321 ccggacctct tccaccggga cattgatggc cgggcccacg atgtggaacg gggggagatg
     1381 gagctgctga gcgatgaact cgccgaggtc ggtttcccag acctcgattc cgtttttttc
     1441 caggagttcg ttgagggcgg tttcttcggt gaccatggat ttccctttgg tcacgtattg
     1501 aattcccttt tccttggcca gattttttat gaactcattg gcctccttgt cgtcccgcgt
     1561 ccagacgact ttcatcccgt tggccgtcgc gtttttctca aactcttcca gaagctccgg
     1621 aaggcgggaa agggcttcgg cccggatcgc cgccccgcag gccatggccg ctggagcatc
     1681 gggaagggag gccaggcccg cctcccgctt ccccttcatc accgggggga tcaatttgag
     1741 gaagaggcgg gagcgatcat tcttcagctc ctttttggcc aattcggtga attgctcggt
     1801 cttaatttcc atattcggcc tccctgccgt tggtggccag gaagctggcc aggtgcataa
     1861 tctttttccc ggggtgatta cggttcaggt agccgccgat attcaagaag cagccgggct
     1921 cgttcagaag caggaggtca gccccgctgg ccaggaagtt ctccaccttc tcctttacca
     1981 tggattcaga gatatccggg aagtcgttgg caaactctcc cccgaacccg cagcaggaat
     2041 cggcgttgtc caggggggcc agttgaaccc ccttcaccgc ttggatcagc tttttgggct
     2101 gctccgaaat cccgagaccc cggaggatgt ggcaggattc gtgataggtc acctttccct
     2161 cgaaggaagc tcccacgtcc tccaccttca gcacatccac cagatactgg gagaattcat
     2221 aaacccgcgg ggcaagctcc ttggcccgtc gaaaccagtc cggttcatcc tttaaaatct
     2281 cggggtagtg gtctttcacc atgtagacgc aagagcctgc gggactcacg atcaccgggt
     2341 ctttctcaaa gacggagata aaatgcttgg ccaccttttt ggcctcttcc cggtacccgg
     2401 cgttcatcgc cggctggccg caacaggtct gctcttcgtg gtagatcgga tcgaccccca
     2461 cgcggcggag gacatgtact accgcctccc caattcgcgg ggcgaagaga tcgaccgtgc
     2521 agggaatgaa aagagatacc tctttcatca ttgactcctt tcgtcttaat acggagcaaa
     2581 tcaagggaga aaaaaattta gaaaaaatga aagcgagcca ccaggcccac ccctattttt
     2641 agcagaataa aaagaaggtg gtcaagggaa aagcgagatg ataaaatccc caacaagcag
     2701 ggtactgtgc ggttgaggaa aagggttgat gtacctctca agactgtgga accgtctttc
     2761 tctactgcga ggaaggagcc ccgtaagcaa tcattttccg tactcatttc aag
//
LOCUS       DASPRR010000422        13859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_418387, whole genome shotgun sequence.
ACCESSION   DASPRR010000422 DASPRR010000000
VERSION     DASPRR010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13859)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13859
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418387"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VEL68_15335"
     CDS             complement(<1..184)
                     /locus_tag="VEL68_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37392.1"
                     /translation="MDDSQFRHVLEFFSLSWKGCRRVRKGVKRRLARYLQDQGFRGME
                     PFLASLEKDPERREEVE"
     gene            complement(293..1096)
                     /locus_tag="VEL68_15340"
     CDS             complement(293..1096)
                     /locus_tag="VEL68_15340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ37393.1"
                     /translation="MIDTHTHIFPDKIAPKIVEKMRTEETWIALSGDFTLKDLKGYMA
                     RCGIEAVFTFCVAERPKAVPPANDFLITITDHRTVFGFGTILPDMEDPVAEVRRIRER
                     GLRGIKFHSLFQPIGAGDENLFPIYEEMARTGMIAYFHVGPAHPSDPARTSPQDVARI
                     KERFPALKIVAAHFGGLFMLDEARKWVIGKDIYIDTCWGPNIQSLEAKEVIDLIRSHG
                     PEKVLFATDYPSTLDPGPQIEWWKVLPLKKEEKDLIFRENARRLLGLSG"
     gene            1418..2176
                     /locus_tag="VEL68_15345"
     CDS             1418..2176
                     /locus_tag="VEL68_15345"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HXZ37394.1"
                     /translation="MGLLASASLATAWWPESHKAIGRNALEVLPEGLKKVFESDPENL
                     SALLWGMVEPDYNRIEDHRIYLKVIRGSVQGPGGVHFALEKYAKQAEEMVRSRKPMNQ
                     IAFVLGQAAHFIQDANVPLHTIYGDTREQHRAYEEAAYVWDWPGDRYGYKGFYLVINY
                     KCFAYETAKRSHPYYDKALKIPPSREVIEKTWNDAVNDTVNLWLSIFYRALGPEKSLE
                     LYGIPVPAHEAGKINRSNGDCEPIPVHDPSTAGR"
     gene            2409..3599
                     /locus_tag="VEL68_15350"
     CDS             2409..3599
                     /locus_tag="VEL68_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37395.1"
                     /translation="MSRNFRWGKMFLSVTVLVIGMIGANAYGQEPIRVGWLSSLTGAL
                     SSPAIAENKGVQFAVDEINKAGGIKGRKIELLTRDTQGDPTKATSYAQQLLKNDKVHF
                     IIGPVHSGECLATVGVIAKAGIPNIIIGTVDILTSPEKYPRAFRVIPTNTQWIDAAND
                     YAMKELGMKKFALLGDNTGYGTSTQEQVEKMLLARGAQITYKAMVDPNQTDVTADMRK
                     AMQSKPDGVQVWSAATGLDARFIEARSHVGWDVTIVGHPAIAAINVGKLLSKPENWSK
                     VFAVGYKSTSYDAGGQLPPRTQEFLKKVEPLLGKEIDYTLWWVVMGYDTVQVVRHGIE
                     KSGSTDPAKWQAAIEDTKNFPGVFATYTWGKNKRDGYPTEELVMNVASSFRNGTYQLA
                     PYKK"
     gene            3931..4797
                     /locus_tag="VEL68_15355"
     CDS             3931..4797
                     /locus_tag="VEL68_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37396.1"
                     /translation="MEIFWAVLVAGIANGAVYTMAGLTYNIMHSTSKVLSMTVGQLVM
                     LGGVGGAFFIGVCNLPIWLGALAVLVLGGVAGFITEWWGVRRVISKIEQHLYVLSTLA
                     LAIMIQEGVGLYWGTEPRPFPDIYGAGWVGLSDPKYWAPLILAVLTMTAIEFFYRRTM
                     YGKAFLAVAQDHFAASARGIPVSRVRVLSYILSGIIGAAAGFGAGKLTFAYFAVGHNF
                     TFYGFIALALGGLGSNVGALVGGIILGIALEAANYLFGGIYMDGVALAILISVLLIVP
                     KGFFGVEGARRV"
     gene            4794..6593
                     /locus_tag="VEL68_15360"
     CDS             4794..6593
                     /locus_tag="VEL68_15360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXZ37397.1"
                     /translation="MKAGKGQKIILGLIAAVLILTLPWWTPNEYWLHVATLIAIFWII
                     NSGLNLVIGYTGVLSVGHVGFFATGAYAAAVLVAKAGVSPWLALLFAGLVAGAVAVVI
                     SLPSLRLQLFYFAMCTLAFFTVVNRLTYGLSWLTGGGIGLPGPGYPGFFGTDHGFYVL
                     VIVLTGFFSWLCWNISRNHPGRALVAIRDSQEAAEALGIPVLYLKVIVFTFSGFLAGV
                     AGALYATHQSYITPEAFTFYLSFYFFVSILIGGRGNTIGPFLGTVILGILPEVSGPLA
                     KLSQFFYGVLLLVVILFVPRGISSVFESWRERRQAEIRMKPPKSEFIRDQIQSPKGMD
                     SVPAVAPLENAADPPPTLKAANLSKKFGSLVAVDGASLAVQPGTIHGMIGPNGSGKTT
                     ILNLLLGYYGVDSGSIHLNGRNMTQWSTRERVIAGLSRTFQTPKVLGELTVIENVMLG
                     LGRQEGRGFFESSLPFGSVRNAEKKMRQEALHLLEGFGMEHMANQRADTLQHSEERFL
                     EIARALAQRPKFILMDEPAGGLSVGEIEILGRILNEIRQSQIGILLVEHHADFVFQVS
                     DVVTALDFGKVIAQGRPEIVQADENVKNAYLGT"
     gene            6893..7657
                     /locus_tag="VEL68_15365"
     CDS             6893..7657
                     /locus_tag="VEL68_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37398.1"
                     /translation="MEKANAITLEAKDLSVAYGKAVVLRRVNLAVPKGGIALLLGRNG
                     AGKTTTLLAIAGLIPKVSGKVFFEGSDISQASPLAIVQAGISNVLEGHRVFVDLTVED
                     NLRLGAFGLRSGKALDERVDRVLEMFPQISPFRAKKAGALSGGQQQILAIAQGLVSSP
                     RLLLLDEPSVGIAPRLVSDILSLIKSLRTEGMSVLLVEQMVQKAMDVADVVYLYDNQS
                     IVGHGAPQEMRASGLVERVYLGREVETSSGRDRLSP"
     gene            7679..8332
                     /locus_tag="VEL68_15370"
     CDS             7679..8332
                     /locus_tag="VEL68_15370"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXZ37399.1"
                     /translation="MGKLNGAAAKRNIFEDLKMSIMQGTFKPRERLIERNLAARFAVS
                     RTPIREALHKLAAMGMVRIIPNQGAMVADFSLQDIESLYFVRLHLERLAGRLACSKVT
                     KEEINTLVTLNQGLKKAMAWDDFSKMVEKDQQFHLTLIRFSKNPFLIKAIEDLRLKSY
                     PFSYYYWRSNQYLRSSLADHNRMIHALRNRDFRLMDTLIEGQLNNSKSRYLKYLAQT"
     gene            8372..9637
                     /locus_tag="VEL68_15375"
     CDS             8372..9637
                     /locus_tag="VEL68_15375"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXZ37400.1"
                     /translation="MKLPSVVKIIQEFEDNSIRDIESHVQGKLEEAGIKKLVKPGQKI
                     ALTMGSRGIRDIARVIKATVEVLKACQAQPYIVLGMGSHGGATVEGQLKIAEKFGVTE
                     KTMGAPIKATMEVVPLGSTASGVPVFLDRYAYEADGIIITHRVKPHRHVMGPHQSGLL
                     KMLTIGLGKLKGAATVHSFGWVNFAKNLPEVSSVVLKKAPVILGLALVENGFSKAAII
                     EAIKPENFIERDRSLLQMAHKMLPRVPFDKIDVLVVKEMGKNMPPDTDIIGKPTLRYY
                     TEVKKPDPSRIVILDLHDDSMGNAIGMGSFDYTTQRLFKKIDFNATAINSIAGNVPEA
                     GKIPLPLENDRKAIEAALQNSGFPDVEGVRDIEQAKLVVIKNTKEMQVLYASQCLAKD
                     INIPEKARVAGSPFDLPFDKQGNIALDFK"
     gene            9670..10137
                     /locus_tag="VEL68_15380"
     CDS             9670..10137
                     /locus_tag="VEL68_15380"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ37401.1"
                     /translation="MQRYDRIFSLIWIALGVFQCAESFSLGLGSVMEPGTGFMPFVMG
                     LVLIALAIALFLESHFATRGTSPTKSSPWSDVHWKRVVYITLIMLGYAVLLPKLGYLL
                     DTFLVMIFLMKSGEPIKWPTALVVGAVTAVFSYVLFGIWLNVSFPEGILSFRG"
     gene            10142..11650
                     /locus_tag="VEL68_15385"
     CDS             10142..11650
                     /locus_tag="VEL68_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ37402.1"
                     /translation="MELLANLYQGFQICFSPINLLYCFVGVLLGTLIGVLPGIGPGAA
                     IALLLPTTFRVSPVSAIIMLAGIYYGAMYGGSTTSILVNIPGEAASVVTCIDGYQMAR
                     QGRAGPALGISAFGSFIAGTLSVVGLMVLAVPLSNVALQFGFPEYFAIMCLGLSIVSY
                     LGRGSIFKSLLMAALGVSLSFVGMDVVVGKPRFDFGLLVLGDGVGLIPLLMGLFGIAE
                     VFSNIEEKIQAREIFQTKIKGLLPTVRDWKDSWAAITRGALLGFFLGILPGGGAVVSS
                     FVSYAVEKKVSKHPEKFGTGAIEGVAGPESANNAATGGAFIPLFVLGIPSNVAMALLL
                     GALMIHGLQPGPLLLQQHPEIFWGTVASMYIGNVMLLVLNLPLIGLWVKLLKVPYKIL
                     FPMILLFCLIGAYSENNNPADMLVMLFFGALGYLLRKYGYDLAPLVLAFVLGRQMEQA
                     FRQSLILSDGSFWVFFSRPISAVAMGLAIFLLVSALIPAFKRKREKIEVDEI"
     gene            11689..12657
                     /locus_tag="VEL68_15390"
     CDS             11689..12657
                     /locus_tag="VEL68_15390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37403.1"
                     /translation="MNRGHRMTWIASLFAFSLLATQAWGAEGFPTKPLELVVPYAAGG
                     STDVMCRTIVARAAPFLNNQPCVVVNKVGGGTVVGSRYVLDGRNDGYTLYSTSTSSMM
                     IAPVVNKTNFSWRDFIGICQTMLGSDALYVRADAPFKTLDQFVEYAKQNPGKIKYSTS
                     GAGGSPHLSVEGLAAAKGISLKQIPTKGDAEAVTALLGGHVMAASGNPIAYQPHVDAG
                     KLRCLAQFSAERDKAFLPNIPTYKELGVDVVVDLWRWVVVPKAVSPEKIKFLREAFTK
                     ILRDKETLVALEKIQCPVSYLPGEDYEKTMSKSEAAIMPLIKAAGL"
     gene            12735..13715
                     /locus_tag="VEL68_15395"
     CDS             12735..13715
                     /locus_tag="VEL68_15395"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37404.1"
                     /translation="MDPKEFIRWTPLLLVIFLLFPTAVGAQTFPGKPLELVVPFAAGG
                     STDVMCRTLASRAAPFLNNQPCIVVNKTGAGSVAAAKYVLDGKSDGYTLMSTSTSSIM
                     VAPVVNKTNYTWRDFVGIAQVMVGSEAFYVRGDSPFDTLEKFIEYARQNPGKIKYGTP
                     GVGSQDHLAMEGFATTRQIVIKHIPTRGDPELIAALLGGHVACVAGSPVAFQPYVSAG
                     KIRCLGQFGTTRDPVVLPNVPTFREQGIDVAVDLWRWIVLPKGVSPDRVKTLGAAFKN
                     IIQDKATLETLTKLQCWVDYQPPEEYERLMGKSEGAVFPLIKLAKILDEK"
BASE COUNT         3047 a         3593 c         3925 g         3294 t
ORIGIN      
        1 tttccacttc ttcccgccgc tcaggatcct tttctaaaga agccaggaat ggttccattc
       61 cccggaatcc ttgatcctgc aggtaccggg caaggcgcct cttcacgccc ttgcggaccc
      121 ggcggcagcc cttccaggag aggctgaaga actcgaggac gtggcggaac tgggagtcat
      181 ccatgggctt ccgcgatcgg gcgaccggcc ggtcgcccct acaaagacta aaccacccac
      241 ggagctgcag acccggcagg cccgcttggg ggcaaaggtg aaggacgcag tttcatccgg
      301 aaagccctag aaggcggcgt gcattctccc ggaagatcag gtctttttct tctttcttca
      361 gggggaggac tttccaccat tcaatctgcg ggccggggtc cagggtgctg gggtagtcgg
      421 tggcaaagag aactttctcg ggcccatggc tgcggatgag gtcaattacc tctttcgcct
      481 ccaaactctg gatgttggga ccccagcagg tgtcgatgta aatatccttc ccgattaccc
      541 acttccgggc ctcgtccagc atgaagagac ctccaaagtg ggccgccacg attttcaggg
      601 cggggaagcg ttctttgatg cgggctacat cctgaggact tgtccgggca ggatcagacg
      661 ggtgggcggg acccacgtgg aagtaggcga tcatcccagt tcgggccatc tcttcataaa
      721 tgggaaacag gttctcatcc cccgccccga tgggctggaa gagggagtga aatttgattc
      781 cccggagacc ccgttcccgg atccttctca cttccgccac cggatcttcc atgtcgggca
      841 gaatcgtgcc gaagccgaag accgtccggt ggtcggtgat cgtaatcaga aaatcattgg
      901 ccggaggcac ggccttgggc ctttcggcca cgcagaaggt gaacactgct tcaatcccac
      961 agcgtgccat gtagcctttc aaatccttca gggtgaaatc cccggaaagg gcgatccaag
     1021 tttcttcggt gcgcattttc tccacgatct ttggagctat cttgtccggg aaaatatggg
     1081 tgtgggtgtc gatcattgaa tttccccttt ccatttcaga ccaaaatggg tattctcctt
     1141 ccttcaaacc tatcatagca aaatttattc gttttagctt acccttttga cttgaaatac
     1201 tcaggtcgaa aggtaaaaat ggaatcttag gtacgcagcg gtaaactgca ttcggcatgt
     1261 tgggaagaag aaattatcct tcatcggagc cgtaggggta tgttgcatcg tgcccttaca
     1321 agctcagggg tgagagatga agtcgatttg ggctttattc aaacaaagtt cctttccaat
     1381 tcacttgaaa ccgttattct cactgggatg catatccatg gggctattgg cttcggccag
     1441 tctggcaacg gcctggtggc ccgagtccca caaggccatc gggcggaatg ccttggaagt
     1501 attgccggaa gggttgaaga aagtatttga atccgaccct gagaatttat cagcccttct
     1561 ctgggggatg gttgagccgg actacaaccg gatcgaggac catcgaatct atctcaaagt
     1621 gatccgaggg tcggtccagg gtcccggagg cgttcatttt gccttggaga aatacgccaa
     1681 gcaagcagaa gagatggttc ggtccagaaa gccgatgaat cagattgctt tcgtgctggg
     1741 gcaggccgcc cacttcatcc aggatgcgaa tgtgccgctt catacgatct atggagacac
     1801 ccgggagcag caccgagctt atgaagaggc ggcctacgtc tgggattggc cgggtgatcg
     1861 atacgggtac aaaggctttt atctggttat aaattacaaa tgctttgctt atgagacggc
     1921 caagcgaagc catccctact acgataaagc cctcaaaatt ccgccatccc gggaggtgat
     1981 cgaaaaaacc tggaatgacg cagtgaacga taccgtcaac ctctggctct cgattttcta
     2041 tcgggccctc ggtccggaga agtcccttga gctttacggg atacccgttc ctgcacatga
     2101 agctggaaag ataaacaggt cgaacggaga ttgtgaaccc attccagttc atgatccttc
     2161 aaccgctgga agataaaatg caggctcaaa tcgccgtaat gctcattgaa gaaaggataa
     2221 gtcactaata ttaagagaaa atgattattt tgatagatct gacttattaa atctattgaa
     2281 gaaaattttc cttgacttgg aattttcctt atgttacgag atggggcata ccaaatggta
     2341 taccatcgca aatcgatacc gatcgttcaa aatgtcgggt ccataaattt catgaaggag
     2401 gttgaggcat gagcagaaat ttcagatggg ggaagatgtt tttatcggta accgttttgg
     2461 taatcggcat gatcggggca aatgcttatg gccaggaacc gatccgggtt gggtggctct
     2521 cctctcttac cggggcccta tcgtcacccg ccattgcgga aaacaagggg gtgcagttcg
     2581 ccgtcgacga gatcaacaaa gccgggggga ttaagggccg gaaaattgag cttctgaccc
     2641 gggatacgca gggagacccc acgaaagcca ccagctatgc ccagcagctc ctcaagaacg
     2701 ataaggtgca ttttatcatc ggccccgtcc actccggaga atgtctggcg acggttgggg
     2761 tcatagctaa agcgggtatt ccgaacatca tcatcggaac ggttgatata ttaacgagtc
     2821 cggaaaaata cccccgggcc ttccgggtga ttccgaccaa cacccagtgg attgacgccg
     2881 ctaacgatta tgcgatgaaa gaactaggga tgaaaaaatt cgccttgctc ggggacaaca
     2941 caggatacgg tacctccacc caggaacagg tagaaaagat gctgctcgcc cggggggccc
     3001 agattaccta taaagcgatg gtcgatccca accagaccga cgtcactgcg gatatgcgca
     3061 aagccatgca gagcaaaccc gacggggtgc aggtgtggtc tgccgccacc ggactggatg
     3121 cccgttttat cgaagctcgg tcccatgttg gctgggatgt caccatcgtc ggccatccgg
     3181 ccatcgccgc catcaacgtg gggaagctgc tctccaagcc ggagaattgg agcaaagtct
     3241 ttgccgtcgg ttacaaaagt acatcctatg acgccggcgg tcaattgccc ccgcggaccc
     3301 aggaattcct gaagaaggtg gaaccactcc tggggaagga gatcgattat accctatggt
     3361 gggtggtgat gggctatgat acggtgcaag tggtccgcca cgggatcgaa aaatccgggt
     3421 cgacagaccc ggccaagtgg caggcggcta tcgaagatac caagaacttt ccgggagtgt
     3481 ttgcaaccta cacctgggga aaaaacaagc gggatggcta ccccacggaa gaattggtca
     3541 tgaacgtcgc cagttccttc aggaatggga cgtaccagct ggctccctat aaaaagtaaa
     3601 ccgctggttg aatatgcttt ttgaaatgac cttcttaaat cgcccctccg cccccctttt
     3661 tcaaagggaa gctgtgaaaa aattgccttt tagtcgtcac cccggtgaaa accggggtcc
     3721 aggcagttct taaggactcg aaaatactag attccggctt ccgccggaat gacgggaaaa
     3781 agaccgagcc cgattttttc acagcttcag gggggaacgg ggggatttct gcagatgctt
     3841 tgcggaaaaa ggagatgtag agaaatatga tgggtttttc aagaagctaa aatgttccaa
     3901 accgcttatt tcccatagga aagggtaccc atggagattt tttgggccgt tctcgtcgcg
     3961 gggatcgcca atggcgccgt gtatactatg gccgggctga cctacaatat catgcattcc
     4021 acctccaagg ttctctccat gaccgttggg cagttggtga tgctcggcgg ggtggggggg
     4081 gccttcttca tcggggtctg caatctgccc atctggctgg gggctctggc cgtactggtc
     4141 ttgggggggg tggccggatt catcaccgaa tggtgggggg tcaggcgggt gatcagtaaa
     4201 atcgaacagc acctctatgt tttaagcacc ctggccctcg ccattatgat ccaggaaggg
     4261 gtgggactct actggggcac cgaaccccgc cccttcccgg atatctatgg ggccggatgg
     4321 gtggggctta gcgacccgaa atactgggcc ccgctcattt tggcggtttt gacaatgacc
     4381 gccattgagt tcttttaccg caggacgatg tatggaaaag ccttcctggc cgtagcccag
     4441 gatcactttg ccgccagcgc ccggggaatt cctgtgagcc gggtgagggt cctgagctat
     4501 attctctccg ggattatcgg ggcggccgcc gggtttggag cggggaaatt gacctttgct
     4561 tactttgccg tgggccataa cttcactttc tatggattta ttgctttggc cttaggagga
     4621 ttgggcagca atgtgggcgc cttggtagga ggaattattc tcggaattgc cctcgaagcg
     4681 gccaactatc tgtttggcgg tatctacatg gatggggtcg ctctggcgat tctgatatcg
     4741 gtcctgctca ttgtgcccaa aggatttttc ggggtggagg gggcacgacg cgtatgaaag
     4801 cgggaaaggg tcagaaaatt atcttggggc tgatcgccgc ggttctgatt ttgactctac
     4861 cctggtggac ccccaacgaa tactggttgc acgttgcgac cttgatcgcc attttctgga
     4921 tcatcaatag cggcctgaac ctggtcatag gatatacggg ggttctttcg gtcgggcacg
     4981 tggggttttt cgccaccggc gcctatgccg cggcggtctt ggtcgccaag gcaggggtgt
     5041 caccatggtt ggccctcctc ttcgccggtc tggttgcggg ggccgtggcc gtggtgatca
     5101 gtcttccctc tctccgttta caactctttt acttcgccat gtgtaccctc gccttcttta
     5161 cggtggtcaa ccgcttgacc tacggactca gctggttgac cggcggggga atcggacttc
     5221 cgggcccggg ctatcccgga ttcttcggca cggaccatgg cttctacgtc ctggtcatcg
     5281 tcttaacggg cttcttttcc tggctttgct ggaacatctc ccggaaccat cccggcaggg
     5341 ctctggttgc catccgggat agccaggagg cggccgaggc cctggggatt ccagtgctct
     5401 atttgaaggt gatcgttttc accttcagcg ggttcctggc cggggttgcc ggtgccctct
     5461 atgcgaccca tcagtcttac atcacgccgg aagccttcac cttctaccta tccttttatt
     5521 tcttcgtctc catcctgatc ggcggaagag gcaataccat cggcccgttt ctggggaccg
     5581 tgatcctcgg aattctcccc gaggtttcgg ggcccctggc aaaactctcc caattctttt
     5641 acggcgtcct gctgctggtg gtcattctgt ttgttccccg gggaatcagt tcggtgttcg
     5701 agtcttggcg ggagcgccgg caagcggaga ttcgtatgaa gcccccgaag tcggagttca
     5761 tccgggacca gattcagtcg cccaaaggaa tggattccgt tcccgcggtg gctccccttg
     5821 aaaacgccgc tgatccaccc ccaactctca aggccgccaa cctgtctaag aaattcggca
     5881 gcctggtggc cgtcgatgga gcttcactcg ccgttcagcc cgggacgatt catgggatga
     5941 tcggcccgaa cggcagcggt aagacaacaa tactcaattt actcctgggg tattatggag
     6001 tcgacagcgg aagcattcat ctcaacggcc ggaatatgac ccagtggtcc acccgggagc
     6061 gggtgatcgc cggcctcagc cggacctttc agactccgaa agtgctgggg gagcttacgg
     6121 tcatcgaaaa cgtgatgctc ggtcttggcc gccaagaggg gcgaggcttt tttgaatctt
     6181 ccctcccctt cgggtccgtt cggaatgcgg aaaagaaaat gcgccaagag gcgctgcatt
     6241 tgctggaagg tttcggcatg gaacacatgg ccaaccaacg cgccgatact ctccagcaca
     6301 gcgaggagag gtttttggaa atcgcccggg cgttggccca gcggccgaaa ttcatcctca
     6361 tggatgagcc ggccggggga ctctccgtcg gagagattga aatcctgggc cggattttga
     6421 acgaaatccg ccagtcccaa atcgggattc tcctggttga gcatcacgcc gatttcgtct
     6481 tccaggtttc ggacgtggtt acagccctgg attttgggaa ggtcattgcc cagggccggc
     6541 cggagatcgt tcaagcggat gagaatgtca agaatgccta tcttggcact tgaggaaaca
     6601 cagacacggc gtggccgcaa caaaaaagcc attaactttt aacgtgtaac gcagggtagg
     6661 ctaccccctc cctaaccatc cccccttgag gggaaggtgt gaaaaaattg ccttttattc
     6721 gtcaccccgg tgaaaaccgg ggtccaggca gttcttaggg cctcgtaaat tctggattcc
     6781 ggcttccgcc ggaatgacgg gaaaaagagc tagccggatt ttttcccacc ttcggggaag
     6841 ggagggtggg gggtaaaatg aattatttca ttcgttagta aagaggggtt ttgtggaaaa
     6901 agcaaacgcg atcactttgg aagcaaaaga tttgtccgtg gcttacggca aggccgtcgt
     6961 tctccgccgg gtaaacctgg ccgtccctaa gggcgggatt gctcttcttc tggggcgcaa
     7021 cggggccggg aagaccacca cgctcctggc cattgccggt ttaatcccga aggtgtccgg
     7081 aaaggttttt tttgagggct ccgacatttc ccaagcttcc cccttggcca tcgtgcaggc
     7141 gggcatcagc aatgtcctgg aagggcatcg ggtgtttgtc gatttgaccg tagaagacaa
     7201 tctgcgcctg ggggccttcg gactcaggtc cgggaaagcc ttggacgaac gggtagatcg
     7261 ggtcctggaa atgttcccgc agatcagccc atttcgcgcc aagaaagccg gggcgttgag
     7321 cgggggccaa caacagatcc tggccatcgc tcagggattg gtttcttctc cccggctttt
     7381 gctcctcgac gaaccctcgg tggggatcgc tccgcgattg gtgtcggata tcctatcgct
     7441 catcaaatct ctgcgcaccg agggaatgtc cgtcttactg gtggaacaaa tggtccaaaa
     7501 agccatggat gtggccgatg tcgtctattt atacgacaac caatcgattg tgggccacgg
     7561 agcgccccag gaaatgcggg ccagcggcct ggtggagcgg gtgtatctgg ggcgcgaggt
     7621 ggagacctcc tccgggagag atcgtctatc gccctaacca ctggttggga tttgcagagt
     7681 gggaaaattg aacggagcag cggccaaaag aaatatcttt gaagatttga aaatgtccat
     7741 catgcagggg acgttcaaac cacgagagcg cctgatcgaa agaaatctgg ccgcacgatt
     7801 tgcagtgagc cgcaccccca tccgggaggc cctccataaa ttggcagcta tgggcatggt
     7861 tcgaatcatc cccaaccagg gcgcgatggt agccgatttt tctctccagg atatcgaatc
     7921 tctctatttt gttcgtctcc acttggaaag gctcgccgga agattggcct gtagcaaggt
     7981 tactaaagag gaaatcaata ccctggtgac ccttaatcaa ggattaaaaa aggcgatggc
     8041 ctgggatgat ttttcaaaaa tggtcgaaaa agaccagcaa ttccacctca ctctcatccg
     8101 tttttccaaa aacccttttc tcatcaaggc catcgaagat ttgcggctaa agtcctatcc
     8161 gttttcctat tactattgga ggagcaatca gtacctgcgg tcttcccttg cggaccataa
     8221 caggatgatc catgcgctcc ggaatcggga ttttcgttta atggatacgc ttattgaggg
     8281 ccaattgaat aactccaaga gccgatattt gaagtactta gcccagacct aacctacagc
     8341 actctttcag ccgagatgaa ggaggtcgac ggtgaaactc ccatcggtcg taaagatcat
     8401 tcaagaattc gaagacaact cgattcgcga catagaaagc catgtccagg gtaaactgga
     8461 ggaggccgga atcaaaaaat tggtgaaacc ggggcaaaaa attgcgttga ccatgggcag
     8521 ccggggcatt cgagatattg cccgggtgat caaagcaacg gtggaggtgc tcaaggcttg
     8581 ccaggcccaa ccttatattg tattaggcat gggaagccat gggggagcga cggtggaagg
     8641 ccaattgaag atcgccgaga agtttggagt gaccgagaag acgatgggcg ctcccatcaa
     8701 agccaccatg gaagtcgtac ctttgggcag taccgcctcg ggggtccccg tgtttctcga
     8761 ccgctacgcc tacgaggcgg atggcattat tatcactcac cgcgtgaaac cccaccggca
     8821 cgtcatgggc ccccaccaga gtggccttct gaagatgctc acgatcggtt tgggaaagct
     8881 gaaaggggcg gctaccgttc actcttttgg atgggtaaat ttcgctaaga atcttcccga
     8941 ggtttcttct gtcgtcttga aaaaagcccc ggtcattttg ggtttggccc tggtggagaa
     9001 tgggttttcc aaggcggcga tcattgaggc aataaaacca gagaatttca tcgagcgcga
     9061 tcggtcctta ctccaaatgg cacacaagat gctcccgcgg gttccctttg acaagatcga
     9121 tgtcctggtc gtcaaagaga tggggaaaaa tatgcctcca gacacggaca ttatcgggaa
     9181 gcctacccta cgatattata ccgaggtgaa gaaacccgac ccctctcgaa ttgttatttt
     9241 ggacctgcac gatgattcga tgggcaatgc cattggcatg ggctcctttg attacaccac
     9301 tcaacgcctt ttcaagaaaa tcgatttcaa cgcgaccgcg attaattcga ttgcgggcaa
     9361 cgtccccgag gccggtaaga ttcctcttcc tctggaaaat gaccgtaagg ccattgaagc
     9421 ggccctgcag aactcggggt ttccagatgt agaaggggtg cgcgatatcg aacaagcaaa
     9481 actggtggtg atcaaaaata ccaaggaaat gcaggtcctc tatgcctccc agtgtttggc
     9541 caaggatatt aacatccccg aaaaggcaag agttgctggc tctccttttg acctcccttt
     9601 cgataagcag gggaatatcg ccttagattt taaatagagc cccccgaaaa ggaggaaagg
     9661 atataccgta tgcaaaggta tgaccgcatc ttcagtctta tatggatcgc cctgggggtt
     9721 tttcaatgcg ccgaatcttt ttccctgggg ctcgggagcg tcatggaacc gggaacgggc
     9781 ttcatgcctt ttgtgatggg gttggtcttg atcgctctgg cgatcgccct ttttttagag
     9841 tcccacttcg cgacaagggg aacatcgccc actaaaagtt ccccttggtc ggacgtacat
     9901 tggaagcggg tcgtctatat taccctcatc atgctaggct atgcggtatt gctgccgaag
     9961 ctcggctatc tgctcgacac cttcctggtg atgatctttt tgatgaaaag cggcgaaccc
    10021 atcaagtggc ccacggccct cgttgtggga gcggtgaccg ccgtcttttc ctatgtgctc
    10081 tttgggatat ggttgaatgt atctttcccg gagggaatat tgagtttccg ggggtaaaaa
    10141 aatggaattg ttggccaacc tttatcaagg gttccaaatc tgcttttccc ccatcaatct
    10201 tttatactgc tttgtgggcg tccttttggg gacgctcata ggagttcttc cgggaatcgg
    10261 gccgggagcc gccatcgctc tgctcctgcc caccaccttc cgggtttccc ccgtgtcggc
    10321 tattatcatg ctggccggaa tctattacgg ggcgatgtat ggcgggtcaa ccacctccat
    10381 cttagtaaac attccagggg aagccgcgtc ggttgtaacc tgcatagatg gttaccaaat
    10441 ggcccgtcag ggaagggcgg gtccggccct ggggatttca gctttcggct cttttattgc
    10501 cgggactttg agcgtggttg gcctcatggt cctggcggtt cctctgtcca atgtcgccct
    10561 gcaatttggc ttccccgaat actttgccat catgtgcctt ggcctttcca tcgtttcgta
    10621 tttgggccgg ggctccattt tcaaatcctt gctcatggcc gcgttgggcg tcagcctgag
    10681 ctttgtgggg atggatgtgg tggtcggaaa gccgcggttc gattttggcc tgctggttct
    10741 gggggatggg gtgggtctca ttcccctgct catggggctc ttcggtatcg ccgaagtctt
    10801 cagcaatatc gaggagaaga tacaggcccg ggaaattttc cagacgaaga tcaaagggct
    10861 actccccacg gttcgggact ggaaagattc ctgggcggcg atcacccggg gggcccttct
    10921 gggattcttc ctcggcatcc tgccgggggg aggggccgtg gtctcctcct ttgtctccta
    10981 tgccgtagag aagaaagttt ccaagcatcc ggaaaaattt ggcaccgggg ccatcgaagg
    11041 agtcgctgga cccgaatcgg ccaataatgc ggccaccggc ggagcgttca ttcctctctt
    11101 tgtgctgggg attccctcca atgtggccat ggcgcttctt ttgggggcct taatgattca
    11161 tgggttgcag ccaggtcccc tcctcctcca gcaacatccg gaaatcttct ggggcacggt
    11221 cgccagcatg tatatcggga atgtcatgct tctcgtattg aacctcccct tgatcggttt
    11281 gtgggtgaaa ttgctgaaag tgccctataa gatccttttt cccatgatcc tccttttctg
    11341 cctgatcgga gcctacagtg aaaataataa tcctgcggat atgctggtga tgcttttttt
    11401 cggggctctg ggatacttat tgcggaaata tgggtatgat ttagcacccc tggtacttgc
    11461 ctttgtgctg gggcgacaaa tggaacaggc ttttcgccag tccttgatcc tctccgacgg
    11521 aagcttctgg gtcttcttta gtcggccgat ttctgccgtg gccatggggc tggcgatttt
    11581 ccttttggtt tccgctttga ttcccgcctt taaacggaaa agggaaaaga tcgaggtgga
    11641 tgaaatttga cggatggctg gcaacacccg gttgagaagg gaggaaaaat gaaccgtggg
    11701 catcggatga cttggatcgc gagtcttttc gcttttagcc tgctggccac ccaggcgtgg
    11761 ggggcggaag gctttcccac taaacccctg gaactggtgg tcccctatgc cgctgggggc
    11821 tcaacagatg tcatgtgcag gaccatcgta gcccgagcgg cgcccttcct gaacaatcag
    11881 ccctgcgtgg tggtcaataa ggtgggggga ggaaccgttg tcggatcgcg ctatgttctg
    11941 gatggccgga atgatggtta caccctttac tcgacttcca cctccagcat gatgattgct
    12001 ccggtggtca acaagaccaa tttttcctgg agggatttca tcggaatctg ccagacgatg
    12061 ctgggttcgg atgccctcta cgtccgggcc gacgctcctt tcaaaacgct ggatcagttt
    12121 gtcgaatatg ccaagcagaa tccgggcaag atcaaatatt ccacctcagg ggcgggtggt
    12181 tccccccacc tttcggtgga gggcctggct gcagccaagg gcatttccct gaagcaaatc
    12241 cccaccaagg gggacgcgga ggccgtgact gctctcctgg gaggccatgt gatggccgcc
    12301 tcggggaatc ctatcgctta ccagccccat gtggatgcgg ggaaactcag gtgcctggcc
    12361 cagttcagcg cggagcggga taaagcgttc ctgccaaata tccccaccta taaagaactg
    12421 ggggtcgatg tggtggtgga cctgtggcgc tgggttgtgg tgcccaaggc cgtgtccccc
    12481 gaaaaaatca agttcctgag ggaagccttt acgaaaatcc tgagggataa agagaccctt
    12541 gtcgcactgg aaaagatcca atgtcccgtt tcttaccttc caggtgagga ttatgaaaaa
    12601 accatgagca aaagcgaagc agcgattatg ccgttaatca aggctgctgg tctgtaaaat
    12661 caataacccc ccgttttgtt cagattcagg atgcgtttgg gggaacggaa tatttgggga
    12721 tgaaaggagc gattatggac ccaaaagaat tcattcgatg gaccccgctt cttctggtta
    12781 tctttctcct cttcccgaca gccgtgggcg cccagacttt ccccggcaag cctctggagc
    12841 tcgtcgttcc ctttgctgcc ggcggttcga cggatgtgat gtgccggacc ttagcctccc
    12901 gggcagcgcc attcttgaac aatcaacctt gcatagtggt caacaagacc ggtgccggga
    12961 gcgttgccgc cgccaaatat gtcctggatg gaaagagcga tggctacacc ctcatgtcca
    13021 cttctacctc cagcattatg gtggccccgg tggtgaacaa aaccaattat acctggcggg
    13081 attttgtggg aatcgctcag gtgatggtgg ggtcagaggc tttctatgtg cgcggggatt
    13141 ctccctttga taccctggag aaattcatcg aatatgcccg gcagaacccg gggaagatca
    13201 agtacggcac cccgggagtc gggagccagg accacctcgc catggaaggg tttgccacca
    13261 ccaggcaaat cgtcatcaaa catatcccca cccgtggtga tccggagctc attgccgccc
    13321 ttctgggagg gcatgtggcc tgtgttgccg ggagcccggt ggcttttcag ccttatgtca
    13381 gtgcaggaaa gatcagatgt ctgggacagt tcggaacgac ccgcgacccg gtcgtcctgc
    13441 ccaacgtgcc cacttttcgg gaacagggga tcgatgtggc ggttgatctc tggcgatgga
    13501 tcgttcttcc caagggagtt tcccccgatc gggtcaagac cctgggcgct gcttttaaaa
    13561 atattattca ggacaaggcc actctggaaa ccctgaccaa acttcaatgc tgggtggatt
    13621 accagccccc cgaggagtac gaacggctca tgggcaaaag tgaaggcgcc gtcttcccct
    13681 tgatcaaact ggccaagatc ctggatgaga aataagttcc tagatggacc ggaaaggagt
    13741 gattatgaag agtcgaagaa ggttgccttg cgccgccttc ctggtaatga tttctttttt
    13801 ggcgggcggt caagcaatgg gggcagagac ttttcccggt aagcccctgg aactcgtcg
//
LOCUS       DASPRR010000423         4741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_104263, whole genome shotgun sequence.
ACCESSION   DASPRR010000423 DASPRR010000000
VERSION     DASPRR010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104263"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..546)
                     /locus_tag="VEL68_15400"
     CDS             complement(277..546)
                     /locus_tag="VEL68_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37405.1"
                     /translation="MRSEFFNSGKRVVIKLITPPRISYEESYAKAFPFNKLLCGMLDL
                     ETIEDTVRTVNEALRDYTQMNRIVNTRAGGNSPLIAQGIADRFHK"
     gene            complement(618..2135)
                     /locus_tag="VEL68_15405"
     CDS             complement(618..2135)
                     /locus_tag="VEL68_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ37406.1"
                     /translation="MDAFSGLWSGFALAVTPVNLLFALIGATLGTAVGVLPGLGPAAT
                     ISLLLPLSYALGNAVTSIIMLAGIYYGAMYGGSTTSILLNIPGEAASVVTCIDGYQMA
                     KKGRAGAALGMAAIGSFIAGTGGVVGLTFVAPPLSAFALKFGPAEYFSLTILGLMLAT
                     YLSGKSVIKGLIMAILGLLLATVGMDPVTGNMRYTFGLLILEDGFDFLTLGMGLFGIG
                     EIFTSLEEKMKAELVTTRITNLWPTLKDWAISKWSVVRGSLIGFFVGLLPGGGAVIAS
                     LLAYAVEKRVSKEPEKFGSGALEGVASPESANNSAASASFIPMLTLGIPGNPAIAMIF
                     AALLIQGVTPGPLLIKDHPHLFWAVIASMYIGNVMLLILNLPLVGLWVQMLKVPYSIL
                     APVIVLICSIGVYSMKNDVTDVSLMVIFGVLGYVLRKLQFELGPLLLAFVLGRILERS
                     LRQALLISRGDISIFITKPISAFLLGIVGLMILTSVIWAIYNRFRPKPAASAKTA"
     gene            complement(2152..2598)
                     /locus_tag="VEL68_15410"
     CDS             complement(2152..2598)
                     /locus_tag="VEL68_15410"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ37407.1"
                     /translation="MKKWERIAAAILTFIGVSAATLAYDLGFGDFHHPGPGFFPFWLS
                     AILALVSFVYFLTQLGADSRRGPLWDKHTWVRPFQAAGVMFLYSLAIGWMGFYSATFL
                     LFLAWLILIEREKWLTVGLVSVLGTFSLFLVFTVFLKVPLPKGVIF"
     gene            complement(2866..3846)
                     /locus_tag="VEL68_15415"
     CDS             complement(2866..3846)
                     /locus_tag="VEL68_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37408.1"
                     /translation="MKKGFQIGLVLSILGIFGLTQAALAQEKYPSKPVEIIVSWAAGG
                     ATDVLFRALGAVFPRYANNQQMIVKNIPGGGAAIGYTEAMKAKGDGYTLVAVATPMIT
                     KIHMSVVKFSATTFAPVILIADNACFLLVHKDSPYKNLKDYIEDAQKRPGQINMGNAG
                     SGGGYHLASLAFANFAKIKLNHIPFEGGGPSITALMGKHVDSIIVSAPEGVPQALAGD
                     LRLLGIFGDRRLDKFPNVPTAKEQGINFNISMWRGISVPKDTPPALITQVHDIVKKCM
                     ADPDFKKKCEELSVELKYMGPEELGKFMVSEDARYQQLIMTEKLGDRYKK"
     gene            complement(3887..>4741)
                     /locus_tag="VEL68_15420"
     CDS             complement(3887..>4741)
                     /locus_tag="VEL68_15420"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXZ37409.1"
                     /translation="ITPLTGDGKINPEALAKLNRFLIDKGIHGLFPCGSTGEGVLLST
                     EERKKVAELTVKEASAKVPVVIHTGSVNMNEALELTLHARDIGADGAALVPPYYFTVD
                     DQTIFQFYSMIAEKVAGFPLYIYNIPPNVKNAVSPKVVSQLQSKYANIVGVKDSSMDF
                     MNFIAFKQAVPEDFVVLMGNDAQIYASLLMGAKGAVAATSTVFPEPVVQIYENLKEGN
                     RDKALKAQEVVIKMRAILRSYTPIAAYKKACEFRGIQAGPPRLPLRPLTPEEAKKLKG
                     ELEALGLL"
BASE COUNT         1155 a         1271 c         1308 g         1007 t
ORIGIN      
        1 tccagaaggt ttggggttag gttgttgggt ttacgtgact aatgagaaag aaacaacaat
       61 tgaaaaatac tctcatcctc ctgggtagtc aagggggaat ttggggggaa aatttgggga
      121 aattgcctcc ccgaggcgcc acttttttct ggccaggagt gatgattggc atttaaaatc
      181 tgcaatactt gtcaaaattt gtcccccgag gattacagga agtactcctt acagcgattg
      241 agagacaaag taaaatcaga gcagtcactt ctggttctac ttatggaacc tgtcagctat
      301 cccctgggcg atcagggggc tgttgcctcc ggcgcgggtg ttgacaatgc ggttcatctg
      361 ggtatagtcc ctcaacgcct cgttcacggt ccggacggta tcctcgatcg tctccaggtc
      421 cagcatgccg cagagcagtt tgttaaacgg gaaggccttg gcataggatt cctcgtaact
      481 tatccgcgga ggtgtgatca gcttgatgac gacgcgcttg ccggaattga aaaactccga
      541 tctcaccttg gcaaattgct tcatcagtgg gggcagccaa ggagaactgc ggataggaga
      601 aaaaaactta gccgcggtca ggcggttttg gcggatgccg cgggtttggg tctgaaccgg
      661 ttgtaaatgg cccagatcac ggaggtgagg atcatcagac cgacgatccc cagcaagaat
      721 gcggaaatcg gtttggtgat aaagatggag atgtccccgc gggagatcag cagagcctgc
      781 cgcagggatc tctccaggat gcgtcccagg acgaaagcca acagaagagg gcccagctca
      841 aactggagct tgcgcaaaac atacccgagg accccaaaga tcaccatcag ggaaacgtcg
      901 gtcacgtcgt tcttcatgct gtaaaccccg atggaacaga tcaacacgat taccggggcc
      961 aggatcgaat aggggacctt cagcatttga acccacagac cgaccagggg cagattgagg
     1021 atgagcaaca tgacgttgcc gatgtacatc gaagcgatca ccgcccagaa gagatgggga
     1081 tgatctttga tgagcagggg gccgggggtg actccctgaa tcaggagggc tgcaaagatc
     1141 atggcaatgg ccggattgcc cggtatgccc agggtgagca tgggaataaa ggaagcgctg
     1201 gctgccgaat tattggccga ttcagggctg gcgactcctt ccaacgctcc ggacccgaac
     1261 ttctccggct ccttcgatac ccttttctcc acggcataag ccagaagaga agcgatcacc
     1321 gcgcccccgc ccggcagcag gccgacaaaa aatccaatga gggaccctct cactaccgac
     1381 catttggaga tggcccagtc tttcagggtg ggccataaat tggtgatcct ggtagtcacc
     1441 aactcggcct tcattttctc ttccagagag gtaaaaattt ccccgatgcc aaaaaggccc
     1501 atgcccaaag tcaggaaatc gaaaccgtcc tccagaatga gcagcccgaa agtgtagcgc
     1561 atattaccgg tcaccgggtc catgccgacc gtagccagca ataaccccag gatggccatg
     1621 atgagcccct tgatgaccga tttgccggac aggtaggtgg ccagcattaa acccagaatg
     1681 gtcagggaaa aatactccgc cgggccgaat ttcagggcaa aagccgacaa gggaggagcc
     1741 acaaaggtca gcccgacgac cccgccggtt ccggcgatga aggacccaat ggcggccatt
     1801 cccagggctg cgccggcccg gcctttcttg gccatctggt aaccatcgat acaagtcacc
     1861 accgaggcag cttccccggg tatgttgagc aggatggagg tggtagatcc cccatacatg
     1921 gcgccgtagt atatgccggc cagcatgata atggaggtta ccgcattgcc cagcgcatag
     1981 ctcagaggaa ggaggaggga aatggtagcg gccggcccca gccccggcaa gactcctacc
     2041 gccgttccca gagtggctcc aatcagggcg aagaggagat tcacgggggt cacggctaaa
     2101 gcaaaaccgc tccacaaacc ggaaaaggca tccaagatac aggctccagg atcaaaaaat
     2161 gacccccttg ggaaggggaa ccttcaaaaa gacagtaaag acaagaaata aggagaaggt
     2221 tcccaaaacg gagaccaggc ccacggtaag ccatttttct ctttcgatca agatcagcca
     2281 ggcaaggaag aggagaaagg tggcggaata aaaccccatc caaccaatgg ccagggaata
     2341 gagaaacatc acccccgcgg cctggaacgg ccggacccag gtgtgtttat cccataaagg
     2401 cccgcggcga gagtccgctc cgagctgggt aagaaaatag acaaaggaga ccagggccag
     2461 aatcgccgaa agccagaagg ggaaaaatcc cggcccgggg tgatgaaaat ctccgaaacc
     2521 caagtcgtaa gccagggtgg ctgcggaaac cccgatgaag gtcaggatag ccgcggcgat
     2581 tctttcccat tttttcataa gagctcttca gccatagagg cacaatgcgg caaagtcgca
     2641 accaaatcac cccctcccta ccctcccccc tcggagactg tgtcgtaatt cgtcatgcca
     2701 gcgaaagctg gcatccagga tttcgaaaaa ttgaataaaa tcaaaaactg gattccggtc
     2761 ggagactcga ctctacgagc tttcgccgga atcacgactc ttttccccaa ttgcgacaca
     2821 gtctttcgag ggggagggtt agggtggagg gccctgaccc tgtccttact tcttataccg
     2881 atcgcccaac ttttcggtca tgatcagctg ctgatatcgg gcgtcctcgg acaccatgaa
     2941 cttacccaat tcctcggggc ccatatattt cagttccacg ctgagctctt cgcatttctt
     3001 cttgaagtcg gggtcggcca tgcatttttt cacgatgtcg tggacctggg tgatcagagc
     3061 cggaggagta tccttgggca ctgaaattcc ccgccacatg ctgatgttga aattgattcc
     3121 ctgctctttg gccgtgggca cattcggaaa cttatccagt cgacggtctc caaaaattcc
     3181 cagaagcctt aaatccccgg cgagcgcctg cggaacccct tccggagcgg aaacgatgat
     3241 ggaatccaca tgttttccca tgagagcggt gatcgaaggc ccgcctccct caaaaggaat
     3301 atgattcaac ttgatcttgg cgaaattggc gaaagcgaga gaggccagat ggtatccgcc
     3361 cccggacccg gcgttgccca tattaatttg cccgggcctt ttctgggcgt cttcgatgta
     3421 gtctttcaga tttttgtaag gtgagtcctt atggaccagc aggaagcagg cgttgtcggc
     3481 gatcaaaatg acgggggcaa aggtggtagc gctgaattta accacgctca tatggatctt
     3541 agtgatcatc ggagtggcta ccgcgaccag ggtatatcca tctcctttgg ccttcatcgc
     3601 ttccgtgtaa ccgatggcgg ctcctccgcc aggtatattc tttacgatca tctgctgatt
     3661 attggcatac ctggggaaca cggcgcccag agccctgaac agaacatccg tcgcccctcc
     3721 cgccgcccag gagacgatga tttccacggg cttggacggg tacttttcct gggccagcgc
     3781 tgcctgggtt agaccgaata tcccgaggat cgaaagaacc agaccgattt ggaatccttt
     3841 tttcattttt ttcctcctta tcctggaagt gaacaattag gaaagtttat aacagaccca
     3901 gtgcttctaa ttctccttta agctttttgg cctcttcggg agtcaggggg cgcagcggca
     3961 gacggggggg accggcctga atcccgcgga actcgcaggc ctttttgtag gcggcgatcg
     4021 gtgtgtagct cctgagaata gccctcatct tgataaccac ctcttgggct ttgagggctt
     4081 tatcccggtt tccctctttc aggttctcgt aaatctgtac cacgggctcc gggaaaaccg
     4141 tgctggtcgc cgcgaccgcc cctttggccc ccatgaggag gctggcgtag atctgggcgt
     4201 cgttccccat gagaacgaca aagtcctcgg gcactgcctg cttgaaggcg atgaagttca
     4261 tgaagtccat gctgctgtcc ttgaccccca ctatgttggc atacttcgat tgcaattgag
     4321 acaccacttt gggggatacg gcgtttttga cattcggagg gatgttataa atataaaggg
     4381 gaaagcccgc cactttttcg gcaatcatac tgtagaactg aaaaatcgtc tggtcatcta
     4441 cggtaaaata ataaggcggg accagagccg ccccgtcggc cccgatgtcc cgggcgtgca
     4501 gggtaagctc gagggcttca ttcatgttga ccgagcccgt gtgaatgacc acgggcacct
     4561 tggcggaagc ttctttcact gtaagctcgg ccaccttctt cctttcctcc gtggacagga
     4621 ggactccctc gccggttgaa ccgcagggaa agaggccgtg gatgcccttg tcaatcaaga
     4681 agcgattgag cttggccagg gcttccgggt tgatttttcc atccccggtt agaggggtaa
     4741 t
//
LOCUS       DASPRR010000424         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_104328, whole genome shotgun sequence.
ACCESSION   DASPRR010000424 DASPRR010000000
VERSION     DASPRR010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104328"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VEL68_15425"
     CDS             <1..725
                     /locus_tag="VEL68_15425"
                     /inference="COORDINATES: protein motif:HMM:NF018427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HXZ37410.1"
                     /translation="TRSTLMSNSSLGGGGFNELRFEDKKGQEEIFLHGQKDWTIAIEN
                     DKNQTVGHDETLQVVRNRSIQVGVNHAESIGANMSLNVGNNRSETVAVASTETVGAAK
                     ALTIGAAYQVTVAGAMNETVGAAKAEEIGGAKVVAVGAQSSESIGGDKSLDTGGDISE
                     NAGKNLTVRSGKKMSFQADDDYSVKGGKKGVVEMADELVLKCGEASITLKKNGDVLVE
                     GKNINVKGSGDVILKGSKVKAN"
     gene            836..1342
                     /locus_tag="VEL68_15430"
     CDS             836..1342
                     /locus_tag="VEL68_15430"
                     /inference="COORDINATES: protein motif:HMM:NF040117.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HXZ37411.1"
                     /translation="MYTKRMEAVGIGERDPQFYGLAPCVGYLAGSSSRGDLLVEHDGY
                     GPNPAKLVKGLCRSELIKEEFRGREVLLVFEKGDPNRPIIINFMADPPEGPLSLVISE
                     SQSVGVKEVYLDGSKITLEAQTEMILKCGLGSITIGKDGKIVIKGTHLVSRSKGVNKV
                     RGATVRIN"
     gene            1429..2469
                     /locus_tag="VEL68_15435"
     CDS             1429..2469
                     /locus_tag="VEL68_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HXZ37412.1"
                     /translation="MGGGSSMLQFENKTPFQAGIAVFPGADAIETLILTIKGTFAIKD
                     TLVLVDEQAPLAHADEYWGEAARSSLKYAAEVHLAKPATDVVMNGHAQVPGQRPVTQL
                     DVHLSVGNLKKIVRVFGDRRWTGGFLSPKMTRATPFETMPLVYERAFGGASSTEGAEG
                     GREFEPRNPVGRGFIGKGKGRDPKDQALPNLEDPVHLISRPTDCPPPAGFGYLAPSWI
                     PRRAFAGTYDESWQKTRAPYLPRDFNPRFFNAAHPDLVCQGYLTGGEPVQIVNASPQG
                     PLRFSLPKTGMAVTVWNAGEEEKPVPRLETVLIEPDESRVFLIWRAVLPCPQGALKVS
                     KVGVSLEKLDLV"
     gene            2626..3009
                     /locus_tag="VEL68_15440"
     CDS             2626..3009
                     /locus_tag="VEL68_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015820381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HXZ37413.1"
                     /translation="MPCTVFANSRGVVHKGSGGMSTVFPDVCKTPSPGGPIPIPYPNV
                     GKSADTVQGPSTVKVDGQMPMVKGAQYMMTSGDEGGTAGGGVLSSSIKGPAEFMMYSF
                     DVKFEGKNACRLGDPLFHNKKNIMG"
     gene            3090..>3604
                     /locus_tag="VEL68_15445"
     CDS             3090..>3604
                     /locus_tag="VEL68_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37414.1"
                     /translation="MNSLVNIIGTGAMTCVGLDAVQTASSVRAGLSRLREIDWQGQDG
                     NPFRGGFLPDDCLEGLNRKTVEILPDSVPARILQLGAPPLREVAEDLPEAPEGFPVIV
                     GIGHQDQLPEASPELFLDNLLRQAQMKINLEASEVVVAGRVAGLMAVHRACLRLAQGL
                     PGPIIAGGIDS"
BASE COUNT          834 a          925 c         1142 g          703 t
ORIGIN      
        1 agactagaag taccctcatg tcaaactcgt ccctcggagg cggcgggttc aacgagctcc
       61 gtttcgagga caagaagggg caggaggaga tcttcctcca tggtcagaag gattggacca
      121 tcgccattga aaacgacaag aaccagactg ttggccatga cgagaccctc caggtggtgc
      181 gtaaccggag tattcaggtg ggtgtgaacc atgccgaatc gatcggggcg aatatgagcc
      241 tcaacgtggg caacaaccgc tcggaaaccg tggccgtggc ttctaccgag acggttggag
      301 cggccaaggc cttgaccatc ggagcggcct atcaggtgac cgtggccggg gccatgaatg
      361 agacggtggg ggcggccaag gccgaagaga tcggcggggc aaaggtcgta gcggttggcg
      421 cccagagcag tgagtccatc gggggtgata aatccctgga cacgggcggg gatatctccg
      481 agaacgctgg aaagaacctc acagttcggt cgggaaaaaa gatgagcttc caggcagacg
      541 atgactattc tgtcaaggga gggaagaaag gggtcgtcga gatggccgat gaactggtcc
      601 tgaagtgcgg tgaggcctcg ataaccttga agaagaacgg cgatgtcctg gtggaaggga
      661 agaacataaa tgtgaaaggg tctggcgatg tcatcctgaa ggggtctaaa gtgaaagcca
      721 actgaggccc atccggcccc cccgtacttt ttcccctgac cagggaagga agtttctgaa
      781 aaatgagaac caatgctcca ggtggaaaaa caaaccacag tttgggagaa gagacatgta
      841 caccaagcga atggaagccg ttgggatcgg ggagagagat cctcaattct atgggctggc
      901 cccttgcgtc ggatatctgg ctggctcaag ttcgaggggc gatctcctcg tagaacatga
      961 tggatatgga ccaaacccgg caaaactagt taagggccta tgcagaagcg agttgattaa
     1021 agaagagttc cgggggagag aagtactgct cgttttcgag aaaggggacc cgaaccgtcc
     1081 catcatcatc aactttatgg ccgaccctcc ggaaggcccg ctatctctgg tgatttcaga
     1141 gagtcaatcg gtcggggtca aggaagttta cctcgacggc agcaaaatta ccctggaagc
     1201 gcaaactgag atgatcctga agtgcggcct aggcagcatc accatcggga aggacggaaa
     1261 gatcgtcatc aaagggaccc atctggtgag ccgatccaag ggagttaaca aggttcgagg
     1321 cgctacggta agaattaatt aggtctctga ctccaggagc cttgccacct ctctcttttc
     1381 ctcgcttcct ccgcctggat ggcaggagag ggacagaaga aggaaggggt gggcggtgga
     1441 tctagcatgc tgcagttcga gaacaagact ccttttcaag cggggatcgc cgtcttcccc
     1501 ggcgccgatg ctatcgagac gctcattctc accatcaagg gaacctttgc gatcaaagat
     1561 accttggttc tggtcgatga acaggccccc ctggcccatg cagatgagta ttggggagaa
     1621 gcggcccgat ccagcctcaa gtatgccgcg gaagtgcatc tcgccaaacc ggctacggat
     1681 gtggtcatga acgggcatgc ccaggttccc ggccagcgtc cggtaaccca gctggatgtg
     1741 cacctatcgg tgggcaacct gaaaaaaatc gtacgcgtct tcggagaccg gcgctggacg
     1801 ggtggcttcc tatcccccaa gatgactcgg gcaactcctt ttgaaaccat gcccctcgtt
     1861 tatgaacggg cctttggagg agccagttcc acggagggag cggaaggagg gagagagttc
     1921 gagccgcgga accccgtggg ccgaggcttc atcggaaagg ggaagggccg ggaccctaaa
     1981 gaccaggccc ttcctaacct ggaagacccg gttcacctga tatcccggcc aaccgattgc
     2041 ccgccgcccg cgggctttgg gtatctagct ccctcctgga ttcccaggag agctttcgcc
     2101 gggacttacg acgaatcctg gcaaaagacc cgcgccccct atcttcccag ggactttaac
     2161 cctcgcttct tcaatgccgc ccacccggac ctcgtttgcc agggctacct gacggggggg
     2221 gagccggttc aaatcgtcaa tgcctcgccc cagggacccc tccgcttttc tttgcccaag
     2281 acggggatgg cggttaccgt gtggaatgcg ggggaagagg agaaaccagt cccccggctg
     2341 gaaacggtcc tgatcgaacc ggatgaatct agagttttcc taatctggcg ggcggtttta
     2401 ccttgtcccc agggggctct caaggtaagc aaggtggggg tgagtttgga gaagcttgat
     2461 ctggtatgag ggtcgaaggt cggccttgaa gaagagagac cctcaagagg aaggtcgtct
     2521 ggagacttag ttccatggtc aggctatggg aatgacaatg cttagccggg aagaatccct
     2581 cataccgatt ccactttggg tcgggtgatt gagcaagggg gaaatatgcc ctgtacagtg
     2641 tttgcgaatt cgcgaggtgt cgtccacaaa ggaagcgggg gaatgagcac agtcttcccc
     2701 gatgtttgta aaacgccctc gcccggggga cctattccca tcccctaccc gaacgtgggt
     2761 aagtcggcgg atacggtcca gggtccgagc acggtgaagg tagacggtca gatgcccatg
     2821 gtgaaagggg cccaatacat gatgacctcc ggggacgaag gaggtaccgc cggcggcggc
     2881 gtgttaagca gctccatcaa gggcccggct gagttcatga tgtactcctt cgacgtgaag
     2941 tttgagggga agaacgcctg ccgtctggga gatcccttgt tccacaacaa gaagaacatt
     3001 atgggataag ggaagagtga agcgaagaag aaagcggtaa tgaacgtcgc gtttagtgga
     3061 gccaagaaag gggtgtttgt agccgggata tgaattctct ggtgaacata atcgggaccg
     3121 gggccatgac ctgtgtcggg cttgacgctg tccagacggc gagttcggtt cgtgccgggc
     3181 tttcccgcct ccgcgagatc gattggcagg ggcaggacgg gaacccgttc cgcgggggtt
     3241 ttctccctga tgattgtcta gaagggttga atcgaaaaac cgtagagatt ctccccgatt
     3301 ccgttcccgc ccgcatcctt caattggggg ctccgccatt gagggaggtt gctgaggatc
     3361 tgccggaagc gccggaaggg tttccggtta tcgtcgggat tggccatcag gaccagctac
     3421 cggaggcttc cccggaactc tttctggaca acctcctgag gcaggcccag atgaagataa
     3481 acctggaggc gagtgaggtg gtcgtcgcgg gcagagtcgc gggtcttatg gccgtacacc
     3541 gtgcctgcct gcgcctggct cagggtcttc ccgggccgat catcgccggc ggcatcgata
     3601 gtta
//
LOCUS       DASPRR010000425        11303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1228434, whole genome shotgun sequence.
ACCESSION   DASPRR010000425 DASPRR010000000
VERSION     DASPRR010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228434"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VEL68_15450"
     CDS             <1..452
                     /locus_tag="VEL68_15450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ37415.1"
                     /translation="SLLGQPITEAAAITDGQTPQKPLENIAVIALRFAGGTVGTVCCG
                     RRVPDTRNDAVIYGTGGRVWLKDTLYEPLKGGLEVVSDSVNMEEPYEGDLLSLYQLEI
                     EAFQRAVHGDEEFEASGIDGVHAVQVASAIIESASTGRTVKIEPLEI"
     gene            857..2395
                     /locus_tag="VEL68_15455"
     CDS             857..2395
                     /locus_tag="VEL68_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37416.1"
                     /translation="MKGKKAGTRRVFILLAIIAMAIVTSVPAQAAPSGVIKQAIHWGL
                     SADWLDPATNPGLISAAITLYLFHDALVKPMPEGNLAPSLAESWTISPDAKVYEFKLR
                     QGVKFHNGDPFTAEDVIFSFWRYKAAQAKTIQGKTEKVEAVNPYLVRFQFKEPFPDFL
                     EYFLPSGSGIGWIAPKKYIEKVGDAEYKKKPVGCGPYKFVEFVAGVRLVAEAFEGYWR
                     KTPHVQRMEFLIISEPATRVAMVRRGEVDIATLIQGVFYEDVKKDSKLRLLHPLSPVT
                     WIVYIGPQFDPKSPWADPRVRKAASLAIDRKSLADIFMPGCKAMGSMAMEGDPFGIPF
                     PPDPYDPPQAKKLLAEAGYPKGFEGGKFYPYQGGYWPYGEQVMTYWKAVGINVETILL
                     DRPAWFANRQGGKMKGVLFIDPAALGTIGSRLGYLFGPTSYGNYPDMKALWDQYQTEV
                     NPKARKDLMERIQKLIHERTMFIPLTDTNSPAAFGPRVKGDPYKVQPLIWFTAPFEDM
                     ELVN"
     gene            2504..2839
                     /locus_tag="VEL68_15460"
     CDS             2504..2839
                     /locus_tag="VEL68_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXZ37417.1"
                     /translation="MSFWVYILRCADRSYYTGHTDNLEKRIVEHQRGEIEGYTSARLP
                     VTLVFADEFPTREEAIARERQIKGWSRKKKEAMMRGDWGEVSRLACRKRPSTSSGRTG
                     EREGWHWNK"
     gene            2906..3961
                     /locus_tag="VEL68_15465"
     CDS             2906..3961
                     /locus_tag="VEL68_15465"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ37418.1"
                     /translation="MLIGWILIFCFCLFSSACSAPQIPKTRLKADKEISSTAKNWFAK
                     GEKLFLRGKYDEALEAFDKAISLAPKFARAYYYRGLVCTKKGQYDKSIEEYSLAIATE
                     PNLSPAYYLRGLTYGRKGHFDRETEDYSKAMATSPNFAPPVFPAQLIKRNLTDGEIEL
                     LSQAITLDPNSAWGYYSRGIAYRMKGQWESAIQDLDRALAIDPHHAWAYYHRAAAWRA
                     KGEYGRAMEDYRLALAEDPNLSWAYYNRGLILRHKSLYEKTVEDFTRALASDPNYSWV
                     YFDRGVTYEMQGAGDKAVDDLSKAMAVDPFFSWTYYHRGLAYLQLGGGRQAILDMQSA
                     ARLENQEAQKWLSERGR"
     gene            complement(3989..5029)
                     /locus_tag="VEL68_15470"
     CDS             complement(3989..5029)
                     /locus_tag="VEL68_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXZ37419.1"
                     /translation="MPRGRKNPELKPSIPASMKAILLDRPGPPEVLRYTDTPTPIPAA
                     GQVLVRAHTIGVSMPEVLVRRGEYAWMPPLPAIPGIEMSGTIVSLGEGVKSLKIGQPV
                     FVSARELPVRAGCYAEYLGADAEALYPLPPGTDLEAAAALSNYQVAWHLLHSAPRAMP
                     YESVLATGAAGGIGSAIVQLGKLSGKRVIGLVDTEEKAAFVRSLADAAINSRGESVIE
                     RVLELTGGKGVDLILDSIGGAGFVTHFARLAPFGLVVSYGQLDGHPIGDVLGAMRKRM
                     GDSLSLRMFSMHTFDQDRRRRREATEELLRLFAAGRIKPLISVRLPLAEAARAHQMIE
                     NGQVLGKLVLKP"
     gene            5467..6327
                     /locus_tag="VEL68_15475"
     CDS             5467..6327
                     /locus_tag="VEL68_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ37420.1"
                     /translation="MKVTEHVHAIKIPFPASTRFVYAYLIYGNQLCMVDSGIAPAKEM
                     VFDYLRKTGRDLKEVRWILQTHSHGDHIGLSSEIKKISGCRVAAHGAEIDWIEDIDKQ
                     YRERPTPTFRTYVQNGTKVDRVVKDGEVLDLDGGESLKVIHTPGHSAGSISFIYSADR
                     ALFSGDAIPAAGGLPIYSDVFVTIQSIKKLKAVKRLKALFSSWHDPIQGEKVYQTMDQ
                     ALAYMQRVHELVRKKNADEPSLEPKELTIRVLKDLRIPENLVNPIVITTIEAHRKVSQ
                     YPNILEPNLK"
     gene            6637..7809
                     /locus_tag="VEL68_15480"
     CDS             6637..7809
                     /locus_tag="VEL68_15480"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ37421.1"
                     /translation="MEPYMKENWFTYFPDNYRWSAGLIIALGTAPWGSSDLGEVDRVG
                     KRLKSKVGDDEAWFIEWTRKGEELENLAKEASSKNRFITASIFNFRACMYFQTGERFR
                     QPKDQKALDAFRRAVACFKEAAKYVDHPSIESVEIPYEGGKSLPGLFVKPSADAKGPF
                     PTVIFFDGLDVTKEYCYFWGVRDLVRRGLACLIVDGPGNGESIRFRGLKLRYDMEVPA
                     GAAVDYLEGRKDVDAKRIGIMALSLGGYYAPRAAAFEKRLKACVAWGAQYDYHALWKR
                     RMEVAYKTPLSVPPDHIQWVLGANSMEDALKRLESFKLAGIAEKIECPFLMVHGEEDA
                     QVSLADAQACFDAVGSKDKTLRVFTSEEGGSQHCQNDRLGVAIPYMHDWLAEKLKA"
     gene            complement(8198..9388)
                     /locus_tag="VEL68_15485"
     CDS             complement(8198..9388)
                     /locus_tag="VEL68_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXZ37422.1"
                     /translation="MGLAKDHPRFSKGEYDRRFREIRKRMHEKGIDVLVLYGDSGSNN
                     SNHANVKYVSNYQDPVSSYAVFPLEGEPSLHISNRLYLPYAKRMSIFPRTDAVDYDPG
                     GAVERRIRELGLEKGTIGLVGLRGILYGSLPFGPLDHWHKAFPRASFVDATDLLAEVR
                     LIKSREELAWFRKGAGLTDLAFEALETKGKAGMTDFQLAGIIANSYMPQGGGIKLIFI
                     GSTSMSRPHLIFPGQFPSHRKVGKGDIILTELSADYEMYAGQAHRPITVGIPPTPIYQ
                     KMFDVALEAYTRVLKSLRPGATHDDVKKAASIIQEEGFTTFDATLHGWGLLIEPPRVD
                     IDAVMIRRPQNEFVVREGMLLVIQPNVVTRDGKRGLQVGNLVEITPKGARALQKFPLK
                     FMRI"
     gene            complement(9425..10897)
                     /locus_tag="VEL68_15490"
     CDS             complement(9425..10897)
                     /locus_tag="VEL68_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37423.1"
                     /translation="MSIGAMAAPPQGVLKQAIHWGLSADWLDPSTASYVTTANHPLYF
                     FHDALLKAMPEGNYTPCLAESWSISPDFKVYEFKLRKGVKFHNGDPLTAEDVVFSYSR
                     YKGALAKFFHGRTEKVEAVNEHLVRIQFKEPLPDFLDYFLPGATTIGWVVPKKYVEKV
                     GDAGFKRNPVGAGPYKFVEFLTGVRLVGEAFEDYWRKVPQVKRMEFHYIPEPATRVAM
                     LRRGEVDVATLLQGVFYEDVKKDSALRLLEPLSPTRWMVYISAQWDPKSPWADPRVRK
                     AASLAIDRKTLADIFMPGCSGVGSLGLDGDPMAEHFAADPYDPAQAKKLLAEAGYAKG
                     FQGGKFYPYGGYWEYGEQIATNWKAVGISVELALLDRPAWLAQRDAGKMKGSLFVDNP
                     IAPTIGARLAYLFSGAGSYGNYPDLQALWDQYQREYSPKARKELIARIQKMTYDRTMW
                     LPLTSTNSPAGFGPRVKGNPYRVQPLLWFTAPFEDIELTK"
BASE COUNT         2616 a         3064 c         3177 g         2446 t
ORIGIN      
        1 actctcttct gggtcagccg atcaccgagg cggcggcgat caccgacgga caaactcccc
       61 aaaaaccgct ggaaaatatt gctgtcatcg ccctccgctt cgctggtggc accgtgggaa
      121 ccgtatgttg cggccggcgg gtgcccgaca ccaggaacga tgcggtaatc tatggtacag
      181 gaggccgggt ttggctgaaa gataccctgt atgaacctct gaagggtggg cttgaagtag
      241 tcagcgattc ggtgaatatg gaagagccct acgaaggaga ccttctgtcc ttgtaccaat
      301 tggagattga agccttccag cgggcagttc atggcgatga ggaatttgag gcttcgggaa
      361 tcgacggtgt gcatgctgtc caggtggcct cggccattat cgaatctgcc tccaccggcc
      421 gcacggtcaa gattgaaccc cttgaaatat aggccgggaa taacgaagcc tcgggtgaaa
      481 acaggaatga gggaaaggtc tggccgacct gcattgtcgt accggccggg caccgtatcg
      541 gcctcaccgt ccggggcaag gattacgagt atgcagggga agggcaaaag ctcaccgcat
      601 tcgtaaacgt gatgaagggg tgcggacttt ttctccatga cgaccctgtt gaccggcctc
      661 cggaggttta cggcgggaaa gtgaccctct acgctggcgg agatcaggca gcctatgtgc
      721 tccttccggt tatcccccag gggaaaggca ccccgtagga aaaagccagg gaagaaaaat
      781 attcggaagg ccaggaatat aaggcgcagc catcagcaaa ttgagaaacc ttttgacctt
      841 aaggagggta aggatcatga aaggaaagaa agctggaacg cgccgggtgt ttatcctctt
      901 ggccatcatc gccatggcca ttgtcacatc ggttcctgct caggcggccc cttcgggggt
      961 aataaaacag gccatccact ggggcctttc ggcggactgg ctcgatccgg caaccaatcc
     1021 gggtctgatc agtgccgcga tcactcttta cctttttcat gacgccttgg tcaagcccat
     1081 gcctgaagga aacctggccc catccctggc cgaatcgtgg accatcagcc cggatgccaa
     1141 ggtgtatgag tttaagttgc gccaaggggt gaagttccac aacggggacc cctttaccgc
     1201 ggaagacgtg atcttcagtt tctggcgata taaagcggca caggccaaaa ccattcaagg
     1261 aaagacagag aaggtggaag cggttaatcc gtatctggtg cgcttccagt tcaaagagcc
     1321 tttccctgat ttcctggagt attttttacc cagcggtagt gggatcggct ggatcgcccc
     1381 aaagaagtac atagagaagg tgggagacgc ggagtacaag aaaaaacccg tcggatgcgg
     1441 tccatacaaa tttgtcgaat ttgtggcggg ggtgcgactt gtggccgaag cctttgaagg
     1501 ttactggaga aagactcccc atgtgcaaag aatggagttc ctcatcattt ccgagccggc
     1561 tacccgcgtg gccatggtcc ggaggggcga agtggatatt gccactctca ttcagggggt
     1621 tttctatgaa gacgtaaaaa aagactccaa gctccgcctg ctccatcccc taagcccggt
     1681 gacctggatt gtttacatcg gtccccaatt tgaccccaaa tcaccctggg cggaccccag
     1741 ggtccgaaag gccgccagcc tggctatcga ccgcaagagt ctggctgaca ttttcatgcc
     1801 cggctgcaaa gccatgggat cgatggccat ggagggtgat ccctttggga ttccttttcc
     1861 cccggaccct tatgaccctc ctcaagcgaa gaagctcttg gccgaggcag gatatccgaa
     1921 gggattcgag ggggggaaat tttatcccta ccaagggggc tattggccat atggagagca
     1981 ggtgatgact tattggaagg ccgtgggcat caacgttgag actatactcc tggaccgccc
     2041 cgcctggttc gccaaccgcc agggcgggaa gatgaagggg gtccttttta tcgaccccgc
     2101 agcgctgggg accatcggat cgcgcctggg gtaccttttc ggcccgacct cgtacggcaa
     2161 ttaccccgat atgaaagcgc tttgggatca ataccaaaca gaggtcaacc cgaaagccag
     2221 aaaggatttg atggagcgca tccagaagct gatccatgaa agaaccatgt tcattcctct
     2281 gaccgatacg aattcaccgg cggcgtttgg cccccgggtg aaaggggacc cgtataaggt
     2341 tcaacccctc atctggttca ccgctccttt cgaagacatg gaactggtga attaagatat
     2401 gacggtcacc cttataatct gatattttgt tgatactccg ttcgccctga gccttcggcg
     2461 tgagctcagt cgaacgcttg tcgaagggcg ggggggccgg ggaatgagtt tttgggttta
     2521 tatattgcgc tgtgccgacc gctcctatta cacggggcac actgataatc ttgagaaacg
     2581 gattgttgag catcagagag gtgagataga aggctacact tctgcaaggc tacccgtgac
     2641 attggtattt gccgatgaat tccccactcg tgaagaggcg atagctcgtg aacggcaaat
     2701 caagggctgg agtcggaaga aaaaggaagc aatgatgcga ggcgattggg gcgaggtttc
     2761 ccgtttagct tgcagaaaac gcccttcgac aagctcaggg cgaacgggag agcgagaagg
     2821 gtggcactgg aacaagtagg gtgcaggagt gagccctcgg gataacattg ttcaaggggt
     2881 aaaaaagaaa attaggaatg gggacatgct cattgggtgg attcttatat tctgtttctg
     2941 tctcttctca tccgcctgct ccgcgcctca gattcccaaa actcgcctaa aagctgacaa
     3001 agaaatttca tccacggcaa agaactggtt tgcaaagggg gaaaaacttt ttctccgggg
     3061 gaaatacgat gaggccctgg aggcgttcga caaggcaatt tctttggccc cgaaattcgc
     3121 ccgggcatat tactatcggg ggctagtttg cacaaaaaaa ggccaatacg ataagtccat
     3181 cgaggaatac agtctggcta ttgccaccga gccgaatctt tcacctgcct actatctccg
     3241 agggctcacc tacggccgca aaggccactt tgaccgggag acggaggact atagcaaggc
     3301 catggccacg agcccaaatt ttgcccctcc cgtttttccc gcccagctga ttaaaagaaa
     3361 cctcacggat ggagaaattg aattactcag ccaggcgatt accctagacc caaattctgc
     3421 ctgggggtat tacagccggg ggattgccta tcggatgaag gggcaatggg aatcagcgat
     3481 tcaagacttg gaccgagcgt tggccataga cccccatcat gcatgggctt attaccaccg
     3541 tgcggcagcc tggagggcca aaggtgaata tggccgggcc atggaagatt atcgcttggc
     3601 cttggccgaa gacccaaacc tctcctgggc gtactacaac cgcggattga tcctgcgcca
     3661 caagagcctg tatgaaaaga ccgtggaaga tttcacccgg gccttagcta gcgatcccaa
     3721 ttattcctgg gtttatttcg atcggggggt cacttatgaa atgcagggcg caggcgataa
     3781 ggccgtagac gacctaagca aggcaatggc ggtagaccca tttttttctt ggacttacta
     3841 ccatcgcggc cttgcttatc tccagctcgg cggaggcagg caggctattc tggatatgca
     3901 gtcggctgcc agactggaga atcaagaagc ccaaaaatgg ttgagcgaaa gagggcggta
     3961 ggcaaagggg ccctcttgtc ggttctggtc acggcttcag aactaacttg cccagtacct
     4021 gcccattctc aatcatctga tgcgcccgcg cggcttcagc cagcggcagc cgaaccgaga
     4081 tcaacggttt gattctgcct gcggcaaaaa ggcggagcaa ctcttcggtc gcctcgcgtc
     4141 ggcggcggcg gtcttggtca aaggtatgca tggaaaacat ccgtaggctt aggctgtcgc
     4201 ccatgcgttt tcgcatcgcg cccagcacat ctcctatcgg atggccgtcg agctgaccgt
     4261 aggagaccac cagtccaaaa ggagcgaggc gcgcgaaatg ggttacaaat cctgcccccc
     4321 cgattgagtc gaggatcaga tcgacgccct tgccgccggt caactcgaga acccgttcga
     4381 tcacgctctc cccccgggag ttgatcgcgg cgtcggccag cgaacgcaca aacgctgctt
     4441 tttcctccgt atccaccaag ccgatcactc gctttccgga aagctttccc agttggacga
     4501 tggcgcttcc gatcccgccg gcggcgcctg tggcgagcac cgattcataa ggcattgcgc
     4561 gcggagcgct gtgcagcaaa tgccaggcaa cctggtagtt ggaaagcgcg gccgccgctt
     4621 ccagatcggt ccccggcggc agcgggtaaa gggcctctgc gtccgcccca aggtactcag
     4681 cgtaacaacc ggcgcgcacc ggaagctcgc gggccgaaac gaaaaccggt tggccgatct
     4741 tcaaggactt caccccctcg cccagggaga caatggtgcc gctcatctca atccccggaa
     4801 tggccggcag cggcggcatc caggcgtact cgccccgtcg gaccaggacc tcaggcatac
     4861 tcacgccgat cgtgtgcgcc cggaccaaga cctggccggc agccgggatc ggcgtcggag
     4921 tatccgtgta gcgcaggact tccggcggac cggggcggtc gagcagaatg gctttcatgg
     4981 aggcggggat ggaaggtttc aattctggat tctttcttcc ccggggcata gcgattctcc
     5041 tttccttctg gatgagttat tactataggc tagagatatt ggggggtcaa gaagacgggc
     5101 tcccctttca ctttatggat tcctccttgt attggcccgg gggccggatc ggaactcgcc
     5161 ggcagaatcg gcaggaggag tctttcctag gaagggactc ctggcttcct ccaagggagg
     5221 cgagggctgc gccggagctg gccacgaaga tggagaggaa cacctcgccc ggtctgatgg
     5281 aatcgagagc gcctcattat gccggcgaga tctgctccgg cccccgggcc gacgggagca
     5341 cttcggagat tcgcacattt tttccccgct ccgggcaaag cgaagtagag gccaagggcc
     5401 cgttgtcctt gaattgggaa tgttgtatga tcctgataaa aatctcaatt tctcaggagt
     5461 tgaaaaatga aagttacgga acacgttcat gccatcaaaa tcccctttcc tgcctccact
     5521 cgattcgttt atgcttacct gatctatggg aatcagttgt gcatggttga cagcggaatt
     5581 gccccggcca aagaaatggt ttttgactac ctgcgaaaga ccgggcgcga tctcaaggag
     5641 gtccggtgga tccttcaaac ccattcccat ggcgaccata tcggtctcag ctccgaaatc
     5701 aagaaaattt caggttgccg agttgctgcc catggagccg agatagactg gattgaagat
     5761 atcgataaac agtaccgcga gaggcccaca cccacctttc gtacctatgt gcagaacggc
     5821 acgaaggtgg atcgggtggt caaggatggg gaggtcctcg acctggacgg cggagaaagt
     5881 ctgaaagtga tccatacgcc cggccattct gccggttcga tctccttcat ttattcagct
     5941 gatagagcgc tgttttccgg agacgccatt ccggcggccg gggggttgcc gatttattca
     6001 gatgtttttg tgaccatcca atccataaag aagctgaaag cggtcaaacg attaaaagcc
     6061 ttgttctctt cctggcacga tcccatccag ggagaaaagg tctaccaaac aatggaccag
     6121 gctttagcct acatgcaaag ggtgcatgag ttggtgagga agaaaaatgc ggatgagcct
     6181 tccctggaac ctaaggagtt aactatccgg gtactaaagg atctgagaat ccccgaaaat
     6241 ctcgtgaacc ccattgtgat cacaaccatt gaagctcatc gaaaggtgag ccaatatccc
     6301 aatattttag agcctaacct gaaataagtt gtgggagtta aatccctctt tgaaaaaggg
     6361 gggaaatggg gggatttgct ttgaaacgcc ttggggaaat cccccctcgt tccgtgaagg
     6421 tttgaaaaaa aatgaatttc agccgtcacc ccggtgaaaa ccggggtcca gttaattcct
     6481 agggctgtga aaacgctgga ttccggcttt cgccggattg acgtgaaaaa gaaccaaatc
     6541 gattttttca cagcttccca tttgcaaaga ggggaatcct attgacggac aagctcctaa
     6601 tccactaagg gatttttctt taataaggag aaggttatgg aaccttacat gaaagaaaac
     6661 tggtttactt actttcccga taattaccgc tggtctgccg ggctgatcat cgccctgggc
     6721 acggccccct gggggtccag cgatcttggt gaagtggacc gggtggggaa aaggctcaag
     6781 agcaaagtgg gggatgacga agcgtggttc atcgaatgga cccgcaaggg cgaagaactg
     6841 gaaaacctgg ccaaggaagc ttcgtccaag aatcggttca tcaccgccag catttttaac
     6901 ttccgcgcct gcatgtattt ccaaaccgga gaacgtttcc ggcagcccaa agaccaaaag
     6961 gcccttgatg ctttcaggcg cgccgtcgcc tgctttaaag aggccgccaa atacgtggac
     7021 catccttcga tcgaatccgt ggagatccct tatgaaggag gaaaatcctt gccgggcctc
     7081 ttcgtcaagc catcggctga cgccaaaggt ccctttccca cggtgatctt tttcgatggc
     7141 ctggatgtga ccaaggagta ttgctatttc tggggagtac gggacctggt tcgccgcggt
     7201 ctggcttgtc tcatcgtgga tggtcccggg aacggggaga gcatccgatt ccggggtttg
     7261 aagcttcggt acgacatgga ggttccagcg ggggcggcag tagactacct ggaaggccgc
     7321 aaggacgtcg atgcgaagcg gatcggcatc atggctctca gcctcggcgg ttattatgcg
     7381 ccccgggctg cggccttcga aaaaaggctc aaggcctgtg tggcctgggg agcccagtat
     7441 gactaccacg cgctatggaa gcggcgcatg gaagtggcgt ataaaacccc gctttccgtt
     7501 ccgcccgatc atatccagtg ggttctgggg gcgaactcca tggaggacgc gctgaagcgg
     7561 cttgaatctt tcaaactcgc gggtatcgcg gaaaagatcg aatgcccttt cctgatggtc
     7621 catggcgaag aggatgccca ggtgtccctg gccgacgccc aggcctgctt tgatgccgtc
     7681 ggttcaaagg acaaaaccct ccgggtcttt accagcgaag aaggcggctc gcagcactgt
     7741 cagaatgacc gattgggagt cgctattccc tacatgcacg actggttagc cgagaaacta
     7801 aaggcctaaa ctcaatcgga ggcagtcatt aagggtaggc cttgttggcg aagtacgtaa
     7861 ttcgtcgcga gtacggtcaa gataaggaag attgagattt gccaagaaat gttgtttagg
     7921 caacccgtcc tttaaatccc cccccaattc ccctttgata agagactgcg tcgtaatccg
     7981 ttcaaggttc gacgagctca ccacgaacgg attatgagct cattacaatt cagtcactta
     8041 tccgctcgcc ctgagcccgt cgaagggcga ccggggaatt atgacacagt ctcgaaaggg
     8101 ggtcaggggg gatttccagc tggggcgtga tcctgggaaa aacctgcttg ctggggtacg
     8161 gctctaccaa tcgatgagtg atttcccaaa agcaatttca aattcgcatg aacttcaggg
     8221 gaaacttctg gagggctcga gcccccttgg gagtgatttc cacaaggttt cccacttgaa
     8281 ggcccctctt tccgtcccgg gtcactacgt tgggctggat gaccagaagc attccctccc
     8341 gcaccacaaa ttcattctgg ggccggcgga tcatgaccgc atcgatgtcg acacggggag
     8401 gctcgatcag aagaccccag ccgtgtaaag tggcatcaaa ggtggtaaac ccctcctcct
     8461 ggatgattga ggcggctttt ttgacatcat cgtgggtcgc ccccggtctg agcgatttta
     8521 agactcgggt gtacgcctcc agggcgacgt caaacatttt ctggtaaatg ggggtgggag
     8581 gaatgcccac ggtaatcggc cggtgtgcct gtccggcgta catctcgtaa tccgcggaga
     8641 gctcggtgag aataatgtcc cccttgccca cctttcggtg agagggaaat tggccgggga
     8701 agatcaggtg gggtcgggac atggacgttg acccgatgaa tatgagctta attcctcccc
     8761 cctgcggcat gtagctattg gcgatgatgc cggccagctg aaaatcggtc attcctgcct
     8821 tcccctttgt ctccagggcc tcgaaggcca ggtcggtcaa acccgcacct ttgcggaacc
     8881 aggcgagttc ctcccggctt ttgatcaggc ggacctcggc caggaggtcc gtcgcgtcca
     8941 cgaaagaggc gcggggaaag gccttgtgcc agtgatccag cggtccaaac gggagggacc
     9001 catagagaat cccccgaagg cccaccaggc cgattgtgcc tttctccagg ccgagctcgc
     9061 ggatgcgtct ttccaccgcg ccccccggat catagtccac cgcatctgtc ctggggaaaa
     9121 tgctcatccg cttggcatag gggagataga ggcggttgga aatgtgaaga ctcggttccc
     9181 cctccagcgg aaagaccgca tagctgctca ccggatcctg gtaattcgat acgtacttta
     9241 cattggcatg attgctgttg ttgctgccgg agtctccgta aaggaccagg acatcgattc
     9301 ccttctcatg catgcgcttc cgaatttccc ggaatcgccg gtcgtactcg cctttggaaa
     9361 aacgcggatg gtctttggcc agacccacgg gtaaccctcc atttcgctgt aagggcaggt
     9421 taagtcattt ggtcagttct atgtcctcaa agggagcagt gaaccacaag agcggctgaa
     9481 ctctgtaagg attccctttt acccgggggc caaaccccgc cggagaattg gttgaagtca
     9541 gcggaagcca catggttcga tcataggtca tcttctgaat ccgcgcgatc agttccttcc
     9601 tggccttggg cgaatactcc cgctggtatt gatcccatag ggcctgcagg tccgggtagt
     9661 tgccatagga ccccgcaccg ctgaaaagat aagccagcct tgctccgatc gtcggggcga
     9721 tgggattatc cacgaaaagg ctccccttca tctttccggc gtccctctgg gccagccacg
     9781 cagggcgatc cagaagagcc aactccaccg aaatccccac ggctttccag ttagtggcaa
     9841 tttgctcccc gtattcccaa tagcccccgt aggggtaaaa ttttcctccc tgaaacccct
     9901 tcgcataccc ggcctcggcc aacagctttt tagcctgcgc cgggtcgtag gggtccgcag
     9961 cgaagtgctc tgccatggga tcaccatcca gtccgaggga accgaccccg ctgcatccgg
    10021 gcatgaaaat atcggccagc gtcttccggt ctatggccaa gctcgcagcc ttccgcaccc
    10081 gcggatcagc ccacggagat ttggggtccc actgcgccga aatgtacacc atccaccggg
    10141 ttgggctgag aggttcgaga agtctaaggg ccgaatcttt tttaacatct tcataaaaaa
    10201 cgccctggag gagcgtagcc acatcgactt ccccccggcg gagcatggcc acgcgggtgg
    10261 ccggctcggg aatataatgg aattccattc ttttcacctg cgggaccttt ctccagtagt
    10321 cctcgaaagc ctcccccacc agcctgaccc ccgtcaggaa ttccacgaat ttgtagggcc
    10381 cggcccccac cgggttcctc ttgaagcccg catctccaac tttttcaaca tatttcttgg
    10441 gaacgaccca gccgatggtg gtcgctccgg gcagaaaata gtccaagaaa tcgggcaagg
    10501 gttccttgaa ttgaatccgg accaggtgct cattgactgc ctccactttt tcggttcgcc
    10561 catggaaaaa tttggccaat gcccccttgt accgagagta actgaaaacc acatcctccg
    10621 cagtcaaagg atcgccgttg tgaaacttta cccccttgcg cagcttgaat tcatagacct
    10681 tgaaatccgg gctgatgctc cacgactcgg ccaggcaggg ggtatagttc ccttcgggca
    10741 tggctttcaa cagggcatca tgaaaaaagt atagcggatg gttcgccgtg gttacatagc
    10801 tggctgtgga agggtctagc cagtccgccg agagccccca gtggatggcc tgtttcagaa
    10861 ccccctgcgg gggagcggcc atcgcgccga tcgacatcaa aaagaatgct ccgaatgaaa
    10921 gcgagaccca taacctccct aacccggtat tccagctttt cattcccttt cccctcccca
    10981 acaaaaaatc cccgatattc ggcccaatcc accggtttct tgaaaaccgc cgttgccttt
    11041 tgactctctt agcatttgat tgaggggagt ctaacagata acgaggggtg tatcaagaga
    11101 gaaaacctca agcggaagtc gcaggttggg aattaaatga tagaaatgtt caacgtccgc
    11161 ccagcgggtc ctttgccact tcgctgtcca gattcatctg aatcatcttg aaattgcggg
    11221 tttcatttta tacaatttga actcctggaa agaccccgcc gatcctgaaa ttggctgtat
    11281 cgaacgcggg ggcccgaagg gaa
//
LOCUS       DASPRR010000426        13496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1228437, whole genome shotgun sequence.
ACCESSION   DASPRR010000426 DASPRR010000000
VERSION     DASPRR010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13496)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13496
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228437"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VEL68_15495"
     CDS             complement(<1..729)
                     /locus_tag="VEL68_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37424.1"
                     /translation="MKKYVIRRLFTVIPVLWLVSVLVFSLIHLVPGDPVLVILGTTAE
                     KAQVESMRHKLGLDRPILVQYGTWVAGLLRGDLGRSIISDEPVMEMILRRMPVTLTVA
                     AAALLLSLSISIPTGILSALRHNTYTDYVFMGLVILAVSMPSFWLALLFILLFSVHLK
                     FLPMMGYVSIFTDFWEGLRYLVLPAMSLAFILAALVARMTRSSMLEVLREDYIVTALS
                     KGTPRRFIVFKHALKNAFAPVLTII"
     gene            complement(807..2366)
                     /locus_tag="VEL68_15500"
     CDS             complement(807..2366)
                     /locus_tag="VEL68_15500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37425.1"
                     /translation="MEKKIWLGFTIVLISVGLICTTVWAAPKRGGTYRSSMATDLTNL
                     DLHKTQAQIDNAVLGMTVYEPLFTYDEKFNVKPFLCESYKMSPDGLNLTLNIKKGVKF
                     HNKTEMKAEDVKFCLDRVRDPNLPGAAQRVSLQNVKEVRVTGPYQVQMVLSKPIPNIF
                     YILATSVGTIGMMPKKVLESHDNKVLKPIGTGPYEFVAWERDTKVILKRFEAYSSADG
                     RVDGYLGKRNRYFDQIIFNVMKEPSTRIMALDRGDLDAVPVLPYPQIDDLKKRKDLQV
                     ITGLAPDAPWYLFNVNFNHPYLSKVDFRKALAYALDRKEITKAAVWGYGTPTFSAIPP
                     MLPAYSPELEKMAPQYDPAKAKEFLEKTGYKGEKIKILTSKNYTPMYDQVLAAQAMWA
                     AVGINTEVEVVDWATHLARWQKGEHEILSFAMIGRMDPLSQTIILGPKNFYNYKNQEA
                     FAIREAMENTWDPDKQNELFRKLYDQTCKDVPFLINFYIHNSIALKPYVKGYENFDLF
                     GGRVWNYYFEK"
     gene            complement(2567..3250)
                     /locus_tag="VEL68_15505"
     CDS             complement(2567..3250)
                     /locus_tag="VEL68_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXZ37426.1"
                     /translation="MTIGYEINDLAKEESWRIFRFIGELVEGFDKLSGVEPAVTIYGS
                     ARIKPGDPLYEQTASIARRLGELGFSIITGGGPGVMEAANKGAKEAGVFSVGLNIHLP
                     EEQAPNPFSTKSITFDHFFIRKVMLVKYAIAFIIMPGGLGTLDELTEVLTLMQTHKIR
                     PFPVILFNSHFWEGFLSWLRNTALPQGFISEEDFKYLGVSDDPAEVVEMVQKWYIKQE
                     TVGRKALRR"
     gene            3596..3892
                     /locus_tag="VEL68_15510"
     CDS             3596..3892
                     /locus_tag="VEL68_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXZ37427.1"
                     /translation="MAKKPMTKSQIVGYFAKKFEVSKKVAAAIIEEVSALAISETKKS
                     GAFVLPGIGKSVIVKRKARMGRNPATGQAIKIPAKTVVKVRVAKAFKEAIVGKR"
     gene            4172..6073
                     /gene="glgB"
                     /locus_tag="VEL68_15515"
     CDS             4172..6073
                     /gene="glgB"
                     /locus_tag="VEL68_15515"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXZ37428.1"
                     /translation="MAPNFLTDHDIYLFREGNHFRLYEKMGAHRTTVGDKEGTHFALW
                     APNAETVSVVGDFNGWNPRANPLGPRWDSSGIWEGFIPGVGKGELYKYHIVSKQNGYE
                     VDKGDPFAFSWEISPKTASRVWDLDYDWNDGDWMKGRHRFNGLKSPLSTYEVHLGSWR
                     RGPQENHRFMTYRELAGALVQYVKDMGFTHVEFLPVMEHPFYGSWGYQTIGYFAPTSR
                     YGTPQDFMFLIDCLHQNGIGVILDWVPSHFPSDEHGLAFFDGTYLYEHADTRKGFHPD
                     WKSLIYNLGRNEVRNFLISSALFWLDRYHADGLRVDAIASMLYLDYSRSEGEWVPNEH
                     GGRENLEAISFLKRMNEIVYGTFPEVQTIAEESTAWPMVSRPAYLGGLGFGLKWNMGW
                     MHDTLEYFSKDPIHRKFHHDQLTFNLWYAFFENFVLPLSHDEVVHMKGSLLGSMPGDD
                     WQKFANLRLLLGYQYTHPGKKLLFMGGEIGQWGEWNHDQSLDWNLLEYTPHRGVQNWV
                     RDLNSFYRSHPALFERDFTPDGFEWIDFKDADHSVISYLRKGDHPEDSLLIVCNFTPV
                     PRQNYRIGVPREGFWKEMLNSDAKEYGGSGWGNFGGIHADPHPSHGKPHSLSLTLPPL
                     SILIFQRVG"
     gene            6199..6579
                     /locus_tag="VEL68_15520"
     CDS             6199..6579
                     /locus_tag="VEL68_15520"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ37429.1"
                     /translation="MKKYVVRPRDLPTYSPPKHSRTTNRRLLGPGRSGSNRLEVVLGE
                     VKYGGQADPHVHPDLEQAFFVLQGKAELEVKGEKAMVGPEDFIFLPPGTVHRVTPLKG
                     PPLKLLIIYSPPLSSSKTPFKEEK"
     gene            complement(7112..7330)
                     /locus_tag="VEL68_15525"
     CDS             complement(7112..7330)
                     /locus_tag="VEL68_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37430.1"
                     /translation="MKQVNVGERLAAQAEVTETDEKKEESLVRGFQPRPAESLRGGVP
                     LPGAGPTPSREKELKKLKSIPTCKDPKY"
     gene            7858..8208
                     /locus_tag="VEL68_15530"
     CDS             7858..8208
                     /locus_tag="VEL68_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HXZ37431.1"
                     /translation="MTFALNAALSALGAYEKKLDVAANNIANVNTDGFKKSRAVLQEA
                     DPSGVVVTISRVNTPGSPILPADGTGEIRESSNVDLAGEIVDLQTTKYGFEANLKTLK
                     TEDEMLGSLFDILA"
     gene            complement(8296..9117)
                     /locus_tag="VEL68_15535"
     CDS             complement(8296..9117)
                     /locus_tag="VEL68_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HXZ37432.1"
                     /translation="MAVITVSRQLGSLGTEIAQKVADVLQYEFVDREKMAKALGTYGL
                     PEPDLERFDEKKPPLWDSLQVQRRKFLHFLQAVIYDYARKGKVIIVGRGGQVLLKDLP
                     GALHLRVVAPLEVRLRRFMESGGIDEKQATRILRRSDRDSSGFIRSFFDVDWDDSALY
                     DLVINTRKLSLDMAVQMILEFIRSPYIEEGGKKAEEKLMDIALLQKVEATLLGILGVE
                     IRHINIQVEGGMVILRGAVGSAMEKGNCEKSVAGLEGVRKVDNQLFVTEYFRFGG"
     gene            9398..10351
                     /locus_tag="VEL68_15540"
     CDS             9398..10351
                     /locus_tag="VEL68_15540"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXZ37433.1"
                     /translation="MKRVLREQAKVYCFDRSLPPQLRVFPGEKFVVETEDASSGYLCK
                     EGQSPLNRPFIDETWPPSANPVAGPIYIEGARRGDLLAIKIEDILVAADRSYTFSARR
                     GPIHDSLKWAQAAEPYTHVLNHKPGPSGTMRDGRVCFNSRVSWPVAPFIGTIAVAPER
                     EVLTSIFGQGIGGGNIDCRDIRPGNTFFVNSQNEGGLLFAGDVHASQGDTEFSGVAAE
                     SRAEVTLSIEVIPGKKISYPRIETPSSLISLYNSRPLEHAVTKAVFLLMEWLVEEYGF
                     SQRDAYMQVSVNPGVRVHVYQMIPEMLLMYTAGVEFPRDCL"
     gene            10500..13079
                     /locus_tag="VEL68_15545"
     CDS             10500..13079
                     /locus_tag="VEL68_15545"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXZ37434.1"
                     /translation="MKDFNLQSITGLTEVEAARRLKADGYNELPPSRQRGIGTIVLEV
                     AWEPMFLLLVTCGVLYLAVGELKEALMLLGFVFVIMGITIYQDRKTERALEALRDLTS
                     PRALVIRDGEQKRIPGREVVREDILVLAQGDRVPADAAILWNENLLLDESLLTGESVP
                     VRKVPWDGVSRIARAGGEDQPFAFSGTLTVQGNGIARVIATGAATEIGKVGKALQSVE
                     TEETLLQKETRRFVRAVALFGVSLCALVVVVYGINLGDWLHGFLAGITLAMAMLPEEF
                     PVVLTIFLALGAWRISKSQVLTRRVPTVETLGSATVLCVDKTGTLTQNRMTVKKIFVQ
                     GEVFEVNPLSPKSLPESFHEVIEFSVLASERNPFDPMEKALRQLGEYTLNDTEHLHDD
                     WTLIHEYPLSRGLLALSHVHRVPGSERYVIAAKGAPEAISDLCHLGPARAQELSQAVQ
                     AMAEEGLRVLGVAKAYFKRAPLPGEQHDFRFELLGLVGLEDPVRPTVAGALQDCYTAG
                     IRMVMITGDYPGTAQNIARQIGLTPLDKVITGAELNQMSDGELQDRVRTVNIFARVMP
                     EQKLRLVNALKANGEIVAMTGDGVNDAPALKSAHIGIAMGERGTDVAREAASLVLLDD
                     DFSSIVRAVRLGRRIFDNLQKAMAYILAIHVPIAGMSLLPVLLKWPLVLFPVHIVFLE
                     LVIDPACSIAFEAEAEEKDIMHRPPRDPQKPLFNWSMVALSLLQGLSVLVILLAVFLF
                     SLSRGQGVQEARALTFTTLVVANLSLILTNRSWSRTIWATLQTANVALGWVFGGALAL
                     LILVLYVPFLRELFHFTFLHPDDLLLCLVAGGFGILWFEGLKLFKKRPKSGLPVNPSM
                     KAG"
BASE COUNT         3081 a         3688 c         3545 g         3182 t
ORIGIN      
        1 gatgatggtc aggacagggg cgaaggcgtt cttcagggca tgcttgaaga cgataaaccg
       61 gcggggggtt cccttggaaa gggcggtgac aatgtaatcc tcccgcagga cctccagcat
      121 ggaagaccgg gtcatcctcg ccaccagggc ggccaggatg aaagccagcg acatagctgg
      181 caggactaga taccggagtc cctcccagaa gtcggtaaaa atggaaacat agcccatcat
      241 gggcaagaac ttgaggtgca cggaaaaaag aaggatgaac agcagggcta gccagaagct
      301 gggcatggaa accgccagga tcaccaggcc catgaaaacg taatccgtat aagtgttatg
      361 gcgaagggcc gagagaatcc cggtgggaat ggatatggaa agggagagaa gcagggccgc
      421 cgcggcgacg gtcaaggtca cgggcatcct gcggaggatc atctccatga ccggctcgtc
      481 ggagatgatc gaacgcccga ggtctcctcg caagaggcct gcgacccagg tcccgtactg
      541 caccaggata ggccggtcga gacccagttt gtgcctcatg gactcgacct gagctttctc
      601 tgccgtagta ccgaggatga ccagaacagg gtcacccggg acgagatgga tcaggctgaa
      661 caccaggacg gaaaccagcc acaggacggg aatgaccgtg aagagacggc ggatgacata
      721 ctttttcata aaaaaagttc cgggtttcga ggctcaagtt tcaagttgac cagaaacccg
      781 gaactcgaaa cccaaaacgc ttctcatcat ttctcaaaat agtaattcca cacccggccg
      841 ccgaacaaat cgaagttctc ataccccttc acgtagggct tgagggcgat gctattgtgg
      901 atgtaaaaat tgatcaagaa ggggacatcc ttgcaggtct gatcatagag cttgcggaag
      961 agctcgtttt gtttgtcggg gtcccaggta ttttccatgg cctcgcggat agcgaaggcc
     1021 tcctggttct tgtaattgta aaaatttttc gggcctagga tgatggtttg agacagaggg
     1081 tccatccgcc cgatcatggc aaaggaaaga atttcatgtt cccccttctg ccaccgggcc
     1141 agatgggtcg cccagtcaac cacctcgacc tcggtgttga tccccacggc cgcccacatg
     1201 gcctgggctg ccaggacctg atcgtacatg ggggtgtagt tcttggaggt caggattttg
     1261 attttttccc ccttataccc cgtcttttcc agaaactcct tagccttggc cgggtcatat
     1321 tggggggcca tcttttccaa ctccggagaa taagccggaa gcatgggggg aatggccgaa
     1381 aaggtgggag ttccatatcc ccacactgcc gccttggtga tctctttccg atccagggca
     1441 taggccaggg ctttgcggaa atccactttt gaaagataag gatggttgaa gttgacgtta
     1501 aagaggtacc atggcgcgtc cggggccagg ccggttatga cctggaggtc ctttctcttc
     1561 ttgaggtcat cgatctgcgg gtacgggaga accggaaccg cgtccaggtc tccccggtca
     1621 agggccatga tccgggtgga gggttctttc atcacattaa atatgatctg atcaaagtac
     1681 cgattcctct ttcccaggta gccgtcgacc cggccatcgg ccgaagaata ggcctcaaag
     1741 cgtttcagga tgacttttgt gtcccgctcc caggcgacga attcataggg ccccgtgccg
     1801 atgggtttca gtaccttgtt gtcgtggctt tccaggacct tcttgggcat catgccgata
     1861 gtccccacgc tggttgccag aatgtaaaaa atattgggaa tgggcttgga gaggaccatc
     1921 tgcacctgat atgggccggt aaccctgact tccttcacgt tctggaggga gacgcgctga
     1981 gcggctcccg gaaggtttgg gtctcgaacc cggtccaggc agaacttcac atcctcagcc
     2041 ttcatctctg tcttgttatg gaatttgacc cctttcttga tgttgagggt gaggttcagc
     2101 ccgtccgggg acatcttgta gctctcgcat aagaaaggct tcacgttgaa cttctcgtcg
     2161 taggtgaaga gaggctcata taccgtcatg cccagtacgg cgttgtcaat ctgcgcctga
     2221 gtcttgtgca ggtccaaatt ggtcaggtcg gtagccatgg atgaacggta cgtccctccc
     2281 cgtttcgggg ccgcccaaac cgtcgtgcat atgagcccga cggaaatgag aacgatcgtg
     2341 aatcccagcc atatcttctt ttccattttt ccctccttct ggaataatcc ttcactctat
     2401 tctggaaatg tttatagtta tccttttgcc tccttcctct caaaccggcc aagataggag
     2461 tataagaaaa gggggaagac cgtgtcaacg agaaagaaaa tcaaaattga ccaccgaact
     2521 caaggggcgt tttccaggaa aagcaatagg tcaaaaataa atccagtcac cggcgcaggg
     2581 cttttcgacc taccgtttcc tgtttgatgt accacttttg caccatctcg accacttcgg
     2641 cgggatcgtc ggatacgcca agatatttga agtcttcttc cgagatgaat ccctggggca
     2701 aagcggtatt cctgagccag cttaagaagc cctcccagaa atggctgttg aaaaggatta
     2761 cgggaaaggg ccgaatctta tgggtctgca tgagggttag gacttcagtg agctcgtcga
     2821 gcgttcccag acccccgggc atgatgatga aggcgatggc gtacttcacc agcatgacct
     2881 tgcggatgaa gaaatgatcg aaggtgatgg acttggtgga aaagggattg ggggcctgct
     2941 cttcgggaag gtggatgttc aagcccacgg agaagacacc cgcctccttg gcccccttgt
     3001 tggccgcctc catgaccccg gggcctccac cggtgatgat ggagaacccg agttccccca
     3061 ggcgccgggc aatactggcc gtttgctcgt aaagcgggtc ccccggtttg attcgggccg
     3121 acccgtatat ggttacggcc ggctcgactc ccgagagttt atcgaatccc tccaccagct
     3181 ccccgatgaa gcggaaaatg cgccaggact cttccttggc cagatcgttg atttcatacc
     3241 cgatggtcat ttggacctcc ccgaaagatt tcatctttta agaaaccgac ggaaatcccg
     3301 ccatcctttt caggctcgct gaatcccttt ggaattggac ctctcgaaaa ccgaacccgt
     3361 tgaaagcttg tcccccggcc gttcgggggg gataaaagca tatggtccta ttctctaacg
     3421 aacaacctgc cggcgcaacc accatcgcag aagaatccag ggttcctgcc gagctcaaaa
     3481 tcgaccccaa aaaacttttt taccattttt actgtttttt cttgattctg gcaatttttt
     3541 caattatact ttgttctgtt ttaccaattt atttctattt ttgaggagga aaagaatggc
     3601 taagaagccc atgaccaaat ctcagatcgt aggttatttc gccaagaaat ttgaggtctc
     3661 caagaaggtg gctgcggcca taatcgaaga agtgagtgct ctggccattt ccgagaccaa
     3721 gaagagcgga gctttcgttc ttccgggaat tgggaaatcg gtcatcgtca agcggaaagc
     3781 ccgcatgggg cgcaaccccg ctaccggcca agccatcaaa atcccggcca aaacggtagt
     3841 caaagtccgc gtggccaaag ccttcaaaga agccattgtg ggcaagaggt aagactctct
     3901 cccggcaagg aaacgggccc gggaaaggca ttgggtttag atatatccca ggggaatttt
     3961 cagcccaatc ggcaaggttg aagtggtttc cctgcgtccg gccaattcga gaaggggtta
     4021 cggtgaatcg taaccccttc ttctttccat agtctccaag aatggtggat ttcagcctca
     4081 aaggatgaat caaggatttc ccctcatcct tccctctccg caaagcttgg gcgagggcag
     4141 gtgaaaaggg gttttccctt cccccacgaa aatggccccc aattttctaa ccgatcatga
     4201 tatttacctc tttcgagagg gaaatcattt tcgtctctac gaaaaaatgg gagcccaccg
     4261 gacgacggtg ggtgataagg aagggaccca ttttgccctc tgggccccca atgcggaaac
     4321 ggtttcagtg gtcggtgatt tcaacggctg gaacccccgg gccaacccgc ttgggccccg
     4381 gtgggacagc tccgggatct gggaaggttt cattcccggt gtaggaaagg gagagctcta
     4441 caaataccac atcgtctcca agcaaaacgg atacgaagtg gataaaggcg atccttttgc
     4501 cttcagctgg gagatctctc ccaagaccgc atcccgggtg tgggacctgg attatgactg
     4561 gaatgacggg gactggatga aaggccgcca ccggttcaac gggcttaaat cccccctttc
     4621 cacctacgag gtccatctgg gttcctggcg gaggggcccg caagagaatc atcgctttat
     4681 gacctaccgg gagctggctg gggcccttgt ccagtatgtg aaggacatgg ggttcaccca
     4741 tgtggaattc ctcccggtca tggagcatcc tttttacggc tcctgggggt accagaccat
     4801 cggatacttt gcccccacca gccgctacgg tactccccag gacttcatgt tcctcatcga
     4861 ttgcctccac caaaacggaa tcggggtgat cctggattgg gttccctccc attttcccag
     4921 tgatgaacac ggcctggcct tttttgacgg cacctacctt tacgaacatg ccgacacccg
     4981 gaagggtttc catccggact ggaaaagcct gatctacaat ctcgggcgca atgaagtgcg
     5041 taatttcctc atcagcagcg ctctcttctg gctggaccgg taccacgcag acggtctccg
     5101 agtggatgcc atcgcctcca tgctttacct ggattattcc cgcagcgagg gagagtgggt
     5161 tcccaacgag cacgggggcc gggaaaatct ggaagccatc agtttcctca aacgcatgaa
     5221 tgagatcgtt tacgggacct tccccgaggt gcagaccatc gccgaagaat ccacggcctg
     5281 gcccatggtc tcccggccgg cttacctggg agggctgggt ttcggcctga aatggaacat
     5341 gggttggatg cacgacaccc tggaatattt ttccaaggat cccatccacc ggaaattcca
     5401 ccacgaccag ctgaccttca atttgtggta tgccttcttc gaaaatttcg ttctccccct
     5461 ctcccacgac gaagtcgtcc acatgaaagg atccctcctg ggaagcatgc cgggggatga
     5521 ctggcagaag ttcgccaacc tccgtctcct cctgggttac cagtacaccc acccggggaa
     5581 aaagctcctt ttcatgggcg gggagattgg acagtgggga gaatggaatc acgatcagag
     5641 cctggactgg aatcttttgg aatacacccc ccaccggggg gtgcagaatt gggtccggga
     5701 cctcaattct ttttaccgca gccatcccgc actgtttgaa agagacttca cccccgatgg
     5761 gttcgaatgg atcgatttta aagacgccga tcatagcgtc atatcttacc tccgaaaagg
     5821 ggatcacccc gaggactctc tcctgatcgt ctgcaacttc accccggtcc cccggcagaa
     5881 ttaccggatt ggtgtgcccc gagagggctt ctggaaggag atgctgaaca gcgatgccaa
     5941 agaatacggt ggaagcggct ggggcaactt tggagggatt catgccgacc ctcacccttc
     6001 ccacgggaag cctcattctc tttccttaac ccttcccccc ttgagtattc taatttttca
     6061 gagagtcgga tgattattct tcaagttcaa gattaacgat tccaagaatc ctaagccctg
     6121 attttttccc gagaggaaga ggaaggacat cttcctgggt tcttgacgga aagaccgaag
     6181 cgaaaaaggg gcgaaaagat gaaaaaatat gtcgtgcgcc cgcgcgacct tcctacatac
     6241 tcccctccca agcattcccg gacgaccaac cgccgccttt tgggtcccgg ccgctcgggc
     6301 tcgaatcgct tggaagtcgt cctgggggag gtcaaatatg ggggacaagc ggacccccat
     6361 gtccacccgg acttggaaca ggctttcttc gttcttcaag ggaaagcgga attggaggtc
     6421 aaaggagaga aggctatggt cggcccggag gatttcatct ttcttccgcc ggggacggtc
     6481 caccgagtca ctcccctgaa agggccgccc ttaaagcttc taataattta ctctccaccc
     6541 ctctcttctt ccaagacccc cttcaaagaa gagaaataat tgcggtcaag gagatgcgtt
     6601 gtcacgcaga ggcatgaaga gggagtttcc ggatggacac taaattaaga cctaagacct
     6661 gaaagcaggg tttccggcta acagctgcag gacccacagg atttctgcat attcgaggtt
     6721 atacggtacc ccgacccccg tgcagtctga atataatcca actggacagg ctttacttct
     6781 tcgtagagtt tcttgttgat cgcgtaggta atcccactct ctttgaaaat ttcatcatcc
     6841 gtatttggtt catccagaac caagcccaat gagggcccgg atcacccgcc gggggctaga
     6901 tagactcgta tcgtgggaag cgtgtcccga tctttgaaat tctcttttag catttctgta
     6961 gctttttcag aaacttgaaa cattttctca ccctccaggc aattaccgcc caattttttt
     7021 cctacttcat tctagcaggt ttctttcatt tttcgaatat attaattagg tgcaaatatt
     7081 cgacaaagga ttcaacaaag gattcatttc cctagtattt tgggtctttg caggtgggaa
     7141 tagatttgag ttttttcaat tctttttctc tagaaggtgt tgggccagca ccaggcaaag
     7201 gaactccccc tcgaagactt tctgctggcc ttggttgaaa acctcgcacc agactttcct
     7261 cttttttttc atctgtttcc gtcacttcag cttgggccgc cagcctctcc cccacattga
     7321 cctgtttcaa aaagcggact tgggaggaaa gaaggaccac gtagggatca ttcaccgcca
     7381 ccatggctgc ataatcgggc agcccaaagg cgaagcccct ggggccaatc cttcttcgtc
     7441 gacccttatt tcagaattta cggttaagga gactcgggag cacttcccgc gatcctctcc
     7501 acttttccca ctaattcacc agatacggac aaatgagttt tttgggtatc gccctcttac
     7561 aaggtgatcg ggttcctcct gacaggaaag tccaatccaa ttggattaat ggagaattta
     7621 atggtaaatg cggctaatgg ggaattcctt cttatttgct tttatgaatt tgaatcgctg
     7681 aaaacttctt ggaactcaac cctttctgct gagtgtcggt cagtgattcg atgttttggg
     7741 ataaattgct tcctaatttc gaggggttag tgtagagatt tgtcccctta ttcagcttaa
     7801 ggatccgcaa tttttgccga tattcttaat tgatttcatt cgccggaggt tgagtttatg
     7861 acttttgctc tgaatgctgc cctgtcggcc ctaggcgcct atgaaaagaa gctcgatgta
     7921 gccgcaaata acatcgccaa tgtgaacacg gatgggttca aaaagagccg agccgttctc
     7981 caggaggccg atccttccgg cgtagtggtc accatcagtc gggtaaacac tccagggtcg
     8041 cccattcttc cagcagacgg caccggagaa atcagggaat cctccaatgt ggatttggcc
     8101 ggagaaattg tcgacctcca aacaaccaaa tatggcttcg aggcgaattt gaagaccctg
     8161 aagacagagg atgagatgct gggctccttg ttcgacattc ttgcctgagg attggggaca
     8221 gagtctacct tcgtttcatc gatttggtgc cctcctttgc cgcgtatcgg agagggtccc
     8281 gcccacaaac gggaatcatc ctccgaaacg aaaatattcg gtcacgaaca actggttatc
     8341 gacctttctt acgccttcta ggccggccac acttttctcg caattcccct tctccatggc
     8401 ggagccgacg gcaccccgca gaattaccat tcccccttcg acctggatgt tgatgtgccg
     8461 gatctccact cccagaatcc caaggagagt agcctccacc ttttgcagga gggcaatgtc
     8521 cataagcttt tcctccgcct tcttccctcc ctcctcaata tacggggaac gaatgaactc
     8581 caggatcatt tgcacggcca tgtccagcga tagcttgcga gtattgatca ctaggtcgta
     8641 gagagcagag tcatcccagt ctacgtcgaa gaaggaccgg atgaaccccg aagaatcgcg
     8701 gtcgctccgg cggagaattc gggtagcctg tttttcatcg atccctcccg attccatgaa
     8761 acgacgtaat cgaacctcca atggagcgac tacccttaag tgtaaggccc cgggaaggtc
     8821 cttcagcaga acctgtcctc cccggcccac gatgatgacc tttcctttcc gggcatagtc
     8881 gtagatcacc gcctgaagga aatggaggaa tttccttctc tgaacctgca aggaatccca
     8941 aaggggaggc ttcttttcgt caaacctctc gaggtccggc tccggaagac cgtaggtccc
     9001 cagagctttg gccatcttct ctctgtcaac aaattcatac tggagaacat cggccacctt
     9061 ctgggcaatc tccgtgccca gactgcccag ctgtctggaa accgtgatca cggccattgc
     9121 tacccccccc tttcgaatac gggataatct aaggtagaat cccaaacccg aaatccccaa
     9181 caaattccaa aaaccaagaa tcccaaagaa gagtccaacc caaccgtttg gaatttgcta
     9241 cttggaattt gatagtttta gggttcttgg ggcttgggat ttggggctca gggtctcatt
     9301 cctcatttct atcttgagcc gcattgaaat gtattataac atttcttcag gctcatttct
     9361 tcacggaccg aaaacccccc aaagaaggag tgtgatcatg aaacgggtct tgcgagaaca
     9421 ggccaaagtc tactgctttg atcgttctct tcctccgcag ttgcgcgtat tccccggtga
     9481 aaaattcgtt gtggaaaccg aggacgcttc cagcgggtac ctgtgcaagg aagggcagtc
     9541 ccccttaaac cggcccttta tcgacgaaac ctggcctcct tcggccaatc cggtggccgg
     9601 gccgatttat atcgaggggg cgcgcagagg agaccttctg gccataaaaa tcgaagatat
     9661 tctggtggct gctgaccgga gctacacttt ttccgcccgc cggggcccga tccacgactc
     9721 cctcaaatgg gctcaggcgg cggagcccta tacccacgta ttgaatcaca agcccgggcc
     9781 gagtgggacc atgcgggatg gccgggtatg ttttaacagc cgggtttcct ggccggttgc
     9841 cccattcatc ggaaccatcg cggtggcccc tgaaagggaa gtcctcacca gtatcttcgg
     9901 gcaaggaatc ggcggcggga acatcgattg tcgggacatc cgccccggaa atactttttt
     9961 cgtgaatagc caaaatgaag gggggctcct cttcgccggc gacgtccacg cctctcaggg
    10021 ggatacagag ttctcggggg tcgctgccga aagccgggct gaagtgacct tgtcgataga
    10081 ggtcattccc ggcaaaaaaa tctcttatcc tcgcatcgaa accccaagca gcctcatttc
    10141 cctttataat tcccggcccc tggaacacgc cgtgactaaa gcggtcttcc tccttatgga
    10201 atggcttgtg gaagagtacg gcttcagcca gcgggatgcc tatatgcagg tgtccgtaaa
    10261 cccgggggtc cgggtccatg tttaccagat gattccggag atgctcctca tgtatacggc
    10321 gggagttgaa tttcccagag attgcctttg aaaaaagcgg caaagaataa agaatatagc
    10381 gcggtgggca ttgctttcaa gacaaatgga taaggattag atttcctgcc catgccgaat
    10441 ggcgtttgct tcaacgctat gcgccatgca ttatgcctcc tgtgcttttt ctgcaagtca
    10501 tgaaagactt caaccttcaa tctatcaccg gcctcactga agtggaagcc gcccggcgct
    10561 tgaaggcaga cgggtacaac gaactccctc cctcccggca gcggggcatc gggacgattg
    10621 tactggaagt ggcctgggaa cccatgtttc tcctcctggt cacctgcggg gtcctctacc
    10681 tggccgtggg ggagctgaaa gaagcgttga tgctcctggg tttcgtcttc gtgatcatgg
    10741 ggatcaccat ctaccaggac cgcaagaccg agcgggccct cgaggctctc cgagacctga
    10801 ccagcccccg ggctttggtc attcgggacg gcgagcagaa gcgcatcccc ggacgggaag
    10861 tggttcggga agacattctc gttctggctc aaggagaccg ggtaccggcg gatgccgcca
    10921 ttctctggaa tgagaacctg ctgctcgacg aatccctttt aacgggtgaa tccgtccccg
    10981 tgcgcaaggt cccctgggat ggggtcagcc gtatagcccg cgccggcggc gaagaccagc
    11041 cctttgcctt ctccgggact ctgaccgttc aaggaaacgg aattgcccgg gtcatagcta
    11101 cgggtgcggc cacggaaatc ggaaaagtgg ggaaagccct gcaatcggtg gagacagaag
    11161 agaccctcct acagaaagag actcgaaggt ttgtccgcgc agtggctctt ttcggcgtgt
    11221 ctctctgcgc cctggtggtg gtggtctatg ggatcaacct gggagactgg ctccatggct
    11281 ttctggctgg aatcactttg gccatggcca tgttgccgga agaattcccg gtggttctga
    11341 ccattttcct ggccttgggt gcctggcgga tctcgaaaag ccaggtcctg acccgtcggg
    11401 tgcccacggt tgaaacatta gggtccgcca ctgttctgtg cgtggataag actgggaccc
    11461 tgacccagaa ccggatgacg gttaagaaaa ttttcgtcca aggagaggta ttcgaagtca
    11521 accctctttc cccgaaatct ttgcccgaat cctttcacga agtaattgaa ttcagcgtcc
    11581 tggccagtga aagaaatccc ttcgacccta tggaaaaggc cctgaggcaa ctgggggagt
    11641 acaccctgaa tgacaccgag catctccatg acgactggac gttgatccat gaataccccc
    11701 tttcccgggg cttgctcgcc ctctcccatg ttcatagagt accagggagt gaaaggtacg
    11761 tgatcgccgc caaaggcgcc ccggaggcca tttcggactt atgccatctg ggtccagcaa
    11821 gggctcagga gttatcccag gccgtacagg ccatggccga agaaggcctg cgggtcctgg
    11881 gagtagccaa ggcctatttt aagcgggccc ccctgccggg ggaacagcat gactttaggt
    11941 tcgagcttct cggtctggtg ggactggaag accctgtccg tcccacggta gccggggcgc
    12001 tccaggattg ttacaccgcg ggaattcgga tggtgatgat caccggagac tatccgggaa
    12061 ccgcccagaa catcgcccgg caaatcgggc ttacccccct ggataaggtt atcaccggag
    12121 cggaattgaa ccagatgagc gatggggaac tgcaggaccg ggtccggacg gtgaatatct
    12181 tcgcccgggt catgcccgaa caaaagctgc gtctggtgaa tgctctcaaa gccaatggag
    12241 aaatcgtggc catgaccggc gacggggtca atgacgcccc ggccttgaaa tccgcccaca
    12301 tcgggattgc catgggggag agaggaaccg acgtggcccg ggaggctgct tccctggttt
    12361 tgctggatga cgatttttct tccatcgtcc gggcggtccg gcttgggcgg aggatttttg
    12421 acaacctcca gaaggctatg gcctacatcc tggccattca tgtccccatc gccggaatgt
    12481 cactcctgcc cgtgcttcta aaatggcccc tggttttatt ccccgtccat atcgtcttcc
    12541 tggagttggt catagacccg gcctgctcta ttgccttcga ggccgaagca gaggaaaaag
    12601 atattatgca ccgtcctccc cgcgatcccc agaagccgct tttcaactgg tccatggtgg
    12661 ccctgagcct gttgcaggga ttgagcgtcc tggtgattct tctggccgtt tttctcttct
    12721 ccctctcccg cggccaggga gtccaggagg cccgcgccct aaccttcacc actctggtgg
    12781 tggccaatct cagcctgatc ctgactaatc ggtcatggtc gaggaccatc tgggctaccc
    12841 tgcaaaccgc caacgtcgcc ttgggatggg tcttcggggg agccctagct ctcctcattc
    12901 tggttctata cgttcccttt cttcgcgagc tcttccattt caccttcctt caccctgatg
    12961 atctcctcct ctgccttgtt gccggaggat tcggcatcct ctggtttgaa gggctcaaac
    13021 tcttcaagaa acgccccaaa tcgggtctac ctgttaaccc atccatgaaa gcaggttgat
    13081 tcccatgcag caaagaaact aagggggtac ccaatcaacg gctacccttt tttgcccatt
    13141 cacttttgag attccatttt tttgggggaa gacctgggat tcgcttattt ccctcctttg
    13201 agaaagagga aggaaggcaa acaccggaaa ggcccttctc cggccgccga tccccaaaac
    13261 tcctggatga caggagtaaa aattttcctt gataaaaaaa gttaaaaagg ttattatcgc
    13321 aacctgactc gccataggta ttttggtttg ttccccggcc cgaaaccttg cccagggatt
    13381 aagggaagaa aatgaagctt aatgcggttg ctttcctgaa agaaaaggtc atcctctacg
    13441 ctctgatggg ccttattctc tggttcttcg cgatccccct ggtaacctac tgtttc
//
LOCUS       DASPRR010000427         6726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_418993, whole genome shotgun sequence.
ACCESSION   DASPRR010000427 DASPRR010000000
VERSION     DASPRR010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418993"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEL68_15550"
     CDS             <1..203
                     /locus_tag="VEL68_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37435.1"
                     /translation="YYFGNLFYSVFSYMEPFFQYKDPGFGREFTRKLLGPLKELFFQK
                     VERGEIDPELNEKLFKFFSAEK"
     gene            701..1927
                     /locus_tag="VEL68_15555"
     CDS             701..1927
                     /locus_tag="VEL68_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37436.1"
                     /translation="MKKFGVALVSVLIFMVAISGTASAKPIAALLFPISGPFSAMGTD
                     MKQGSEIAVEEINAKGGVLGQKAEIVTGDDEFKAPVAIRKYKEMVDGQGIKAIGGTLS
                     GGIAVAVNEWACKNKVLYMSFCHSSIPLGKEQCGYGFVSGMIPYQTGVATAKYAFTHL
                     GKSWMIITQDYRFGTDELSAWLVTSEKMGGKFLGNIYSPLGQTDYSAHIPKIVATNPE
                     ILVLNVYGQSLGAIIKQLGEAGLNTKKMKFVMPKSHIWEIKAAGAIYNENFYGGHTYY
                     WTLQDKFPKAKPFNEAYGKKFGMPPSQDADGGYTGTRALLEAMNAAKTTTDVDKLVKT
                     LEGFKMDYNKGPEQFRACDHIRETSVVIVRGIGAKAKDWNFGDLVAEYPVSETLESCE
                     NNKKDVPYGAVKLPGK"
     gene            2194..3072
                     /locus_tag="VEL68_15560"
     CDS             2194..3072
                     /locus_tag="VEL68_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37437.1"
                     /translation="MELNATLIINQMLFGLALGAVYILMACGLTIIFGLLDVVNFAHG
                     TFYMLGAFAAFVIDSLFGNFWLGLASAVVIVGLIGWITESYLIKPLYGKDPLYPLMLT
                     FGFSVAVTDLMRGIFGTIGRIVPLPQIFTGSLMWEGIFIPKYRVFIIFLTLGVLLFLW
                     FFFKKTSLGMIIRAATRDSLMVRTLGINVPRIWTFGFSLGVGLAALAGGVNSTVQSAL
                     PDMGVEMTIECFVVVVIGGLGSLGGAILGGLIIGQVISFVSLLASDWSNVAIFITMLL
                     ILLVRTRGLFGEAGRL"
     gene            3099..4121
                     /locus_tag="VEL68_15565"
     CDS             3099..4121
                     /locus_tag="VEL68_15565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37438.1"
                     /translation="MATKMKTVKVQTSNRVVWITLLIFLVVPYLVPGNIISTALATEI
                     MISGLFALGFNLLLGYTGILSFGHAAYFGLGCYGCGIAIRDFGLSVWGGLFVSLILGV
                     ILAATVGAIAIKKRGVYFAMISMAIAQMFYFMALSPFKRWTGGEDGLKFIPKLSLSFP
                     FAVDLQSPIPLYYFVYFIVGISILAIWRILNSPFGRLLQAIRENEPRTLASGYNVTLA
                     KFISLVFSGLFSSLAGGLFCIYLGYGPLQTLYWLTSGTILLMTILGGMHTFIGPVVGV
                     GVFLYAQNKFSWILERWELVVGILFVALILIFPEGIVGTIKSKYLARRTASQEVEPVE
                     ETASRH"
     gene            4125..4877
                     /locus_tag="VEL68_15570"
     CDS             4125..4877
                     /locus_tag="VEL68_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006078208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37439.1"
                     /translation="MPYLETKGLCKYFGGLKAVDQVNFSVEKGQLKSIIGPNGAGKTT
                     LFNLLCGLYEPTAGKIFFQGEDITRKKMNRTSQLGITKTFQITHIFPRLSVFENVRIS
                     AQSRKTSFNFWRKADALEEINEKTRKILRMVQLLDSQDRPASILSHGERKYLEIGIAL
                     AADPKLLLLDEPTAGMSPAETVLAMNLIRNLRDQLNLTILLVEHDMNVVMNISDEVFC
                     LNEGRVLACGTPEAISADASVQKVYLGGTKAC"
     gene            4871..5593
                     /locus_tag="VEL68_15575"
     CDS             4871..5593
                     /locus_tag="VEL68_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37440.1"
                     /translation="MLAVENINTYYGTSHILHDVSLDVQDGEIVVLLGRNGVGKTTTL
                     KTIMGIQPCRSGKVTFNGQNITGFPPDEIARRGISLVPEDRRVIPNLTVFENLKLGML
                     VRMKREKVDLPETLEMVFHYFPRLKERLNQLGESLSGGEQQMLTTARGLVANPKLMII
                     DEPTEGLAPILVEEIWEMLKKLHENKVTILLVEQNYEKSLQLSKNLRAYILEKGHIRM
                     SGKRGELHERQEEVEKYLGVKL"
     gene            5699..>6726
                     /locus_tag="VEL68_15580"
     CDS             5699..>6726
                     /locus_tag="VEL68_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ37441.1"
                     /translation="MSKDSVVKYDERGVLDGGTVGLPRKMTREIWLEEVFPEWGTYLN
                     YEIDQFKVKPKTGAMWYFGGPSFALKSQGGAVFLVDLYAGPSLFTDYSYCGVCRTSGA
                     DKLYWMRLNPHVIDPWKFKRLDAVCITHHHQDHMDFYTIQAALQTTKCKFIAPPETCR
                     RMKKNMGVPEDRIIVTKPGKSVTIEDMKIDFAPNFDVIVTKTGFETPQPFEEVAVSFI
                     FNTQGGGIAFLGDTLYHNGYRMVGEKYKIDVVCMDMGNNAPGYTDKMSPWDVWRVAEA
                     MNAKVVIPDHHDNWANCYEDPSYLVDIVNRKNKEKRPEMKTVILLPGARYEHPTDKNM
                     GRYVYPDWR"
BASE COUNT         1556 a         1715 c         1852 g         1603 t
ORIGIN      
        1 attattattt cgggaacctc ttctactccg tcttctctta catggaaccc tttttccagt
       61 acaaggaccc gggtttcggc cgggagttca cccggaagct gctcggtcct ctgaaagagc
      121 tatttttcca gaaagttgaa aggggggaaa tagacccaga gctgaatgag aagttattca
      181 aattcttttc ggccgaaaaa taggacctgg atgaaatcat atttcagact ggaaagatca
      241 gggtgagcct aacgtgcact tccgaatcct ttgacccttt cttctgattg cttgacttcc
      301 ctgaacccgt catatatata taattctgaa gttctaatca tagtttttcg gatgaacaac
      361 caatttgtcg atcgcccaag agcgggcagg tttcagatgc ctatttattc agcgcccttc
      421 cggtgattcc gaaaaatttt tcatcgccga tttgaggcgg acccgctttt tcttgcagtt
      481 ctgatccctg cgcgtaagtg agttcatcca cccctgcgtt ttggaaaagc tggtgcggct
      541 atccttcttt cgggggcggg aaagcaggcg gtgggatagg gtacctggct gagcgaaatt
      601 ggatgcgcta gaggggaaac ctattttcag aagggacgat gggacaaagg aagagagcag
      661 aattcctttc agcatactaa aacgataagg aggtcagggt atgaaaaagt tcggtgtggc
      721 tctcgtatcg gttcttatct tcatggtggc gatctccggc acagcttcgg cgaagccgat
      781 tgccgccctg ctgttcccga tttccggtcc tttctcagcc atggggacgg atatgaagca
      841 aggttcggaa atcgccgttg aagagatcaa cgccaagggt ggcgtcctgg gacaaaaagc
      901 ggaaattgtc acgggggacg acgagtttaa ggcgccggtg gccatccgca agtacaaaga
      961 gatggtcgac ggccagggaa tcaaagccat cggcggaacc ctctccggcg gaatcgccgt
     1021 ggcggtcaac gaatgggcat gcaagaacaa ggtcctttat atgtcctttt gccatagcag
     1081 cattcccctg ggaaaggagc agtgcggcta cggctttgtc tccggaatga ttccctacca
     1141 gaccggtgtc gctaccgcca agtatgcctt cacgcacctg ggtaaatcct ggatgatcat
     1201 cacccaggac tatcgtttcg gcacggatga actttccgcc tggctggtga cgagtgaaaa
     1261 gatgggcgga aaattcctgg gaaatatcta ctctcccctg ggacaaaccg actactccgc
     1321 ccatattccc aagattgtgg ccacgaaccc ggaaatctta gtcctcaatg tctatggcca
     1381 atccttgggt gcgatcatca agcagttagg tgaagccgga ctcaacacta agaagatgaa
     1441 atttgtcatg cccaagagcc atatttggga aatcaaggcg gctggcgcca tttacaatga
     1501 aaatttctac ggcggccaca cgtactattg gaccctgcag gacaaattcc ccaaggccaa
     1561 gccgttcaat gaggcctatg ggaagaaatt cggcatgccc ccctctcagg acgcggacgg
     1621 cggatacacg gggacccggg ctttgttaga ggccatgaac gcggcgaaga cgaccacgga
     1681 tgtggacaaa ctggttaaga ccctagaagg gtttaagatg gattacaaca agggccccga
     1741 gcagttccgg gcctgcgatc atatccggga gacaagcgtc gtcatcgtaa gggggatcgg
     1801 ggccaaggcc aaggattgga acttcggaga ccttgtggcc gaatatcccg tcagcgagac
     1861 cctcgaaagc tgtgaaaaca ataagaagga cgttccctac ggggctgtca aacttccagg
     1921 gaagtaagta tacccaatcg tcgcataata attctggcaa ttcgcaagaa agcattgctc
     1981 cttccccccc caccctaacc ctccccctcg aggggggagg gaagggaggg ggtgattgct
     2041 tgccccccat tccagtgcgg ggtaggtccg gaatccagct ttttcctgga ttcgcgcctc
     2101 tgcaggatga gacgcttgga tgtgattcat ttcactcagt aggaaagctc ggaggcttgg
     2161 gattctcccc caagcctctt tctaaggtag ggaatggaac tcaacgcaac cttgattatc
     2221 aatcaaatgc tcttcggcct cgccctgggg gcggtataca tcctgatggc ctgcggactc
     2281 acgattatct ttgggctgct ggatgtggtg aactttgccc acggcacttt ttacatgttg
     2341 ggggcctttg cggcctttgt gatcgattcc ctgtttggaa atttttggct agggttggcg
     2401 agcgccgtag tcatcgtagg cttgatcggg tggatcacgg aatcgtatct catcaagccc
     2461 ctctatggca aagaccccct ctacccatta atgctgacct ttggattttc cgttgccgta
     2521 accgacctaa tgcgcgggat cttcgggacg attggccgga tcgtccctct tccccagatt
     2581 tttaccggct ccctgatgtg ggaaggaatc tttatcccca aataccgggt cttcattatc
     2641 tttttaacct tgggagtcct gctattcctc tggttttttt tcaagaagac cagtttggga
     2701 atgattattc gcgcggctac ccgcgacagc ctcatggtgc ggaccctggg aatcaacgtg
     2761 ccgaggatct ggacctttgg gttctccctg ggggtcggtc tggcggcttt ggccggaggg
     2821 gtcaactcca ccgttcaatc ggctctgccg gatatgggcg tggagatgac cattgaatgc
     2881 ttcgtggtgg tggtcatcgg cggtttgggc agcctgggcg gagcgatcct tggaggattg
     2941 atcatcgggc aggtcatcag tttcgtctcc ctcttggcca gcgactggtc caatgtcgca
     3001 attttcatca ccatgctttt gattctcctg gtgcgaaccc gcgggctgtt cggggaagcc
     3061 ggaagattat aaaatgaggg cttggctgga aagttagaat ggccaccaaa atgaaaaccg
     3121 taaaggtaca gacgagcaat cgggttgtct ggattaccct gctgattttt ttggtggttc
     3181 cttatttggt tcccgggaat atcatctcga ccgccttggc gacggaaatc atgatctcgg
     3241 gcctttttgc gctgggcttt aatctccttc tcgggtatac gggaatcctt tcctttggcc
     3301 acgcggcgta tttcgggctg ggatgctatg gctgcgggat cgccatccgg gatttcggct
     3361 tgtcggtctg gggagggctt ttcgtaagcc tcatcctggg ggtgatcctg gccgcgaccg
     3421 taggcgccat cgccatcaaa aaacggggcg tttatttcgc catgatctct atggccatcg
     3481 cccagatgtt ctatttcatg gcgctttccc cctttaaaag atggaccgga ggggaagacg
     3541 gtttgaaatt cattcccaaa ctgagtcttt cctttccgtt tgccgtggac ctgcaaagtc
     3601 cgattcctct ctactatttt gtttatttca tcgtaggaat ctctatcttg gcgatctggc
     3661 ggattctgaa ctctcccttt ggccgtctgc tccaggccat ccgggaaaat gaaccccgga
     3721 cccttgcctc aggatacaat gtgaccctgg ccaaatttat ctccctggtc ttctcggggc
     3781 tcttctcctc cctcgccggg ggattattct gtatttatct gggctacggt cctttgcaaa
     3841 cactctattg gcttacttcg ggaacgatcc tgctcatgac catcctgggg ggaatgcaca
     3901 cgtttatcgg tccggtcgtg ggggtaggag tttttctcta cgcccagaat aaattcagtt
     3961 ggatattgga acgctgggaa ttggtggtgg ggatcctgtt cgtcgctctg atcctgatct
     4021 tccccgaggg aatcgtggga acgatcaaat cgaaatattt ggcgcggagg accgccagcc
     4081 aggaggtcga accggtagaa gaaacggcgt cgaggcacta aactatgcct tatttggaaa
     4141 ccaaaggtct gtgcaaatat ttcggcggat tgaaagcggt cgatcaggta aatttttccg
     4201 tggaaaaagg ccagctgaag tccatcatcg gacccaatgg agccgggaag acgacccttt
     4261 tcaatcttct ttgcgggctc tacgagccga ccgcgggaaa gatcttcttc cagggggagg
     4321 atattacccg caagaagatg aaccgcacat cccagctcgg gattaccaaa acattccaaa
     4381 tcacccatat ctttcctcgc ctctcggtct ttgagaatgt ccgcatttcg gctcaatccc
     4441 gcaagacttc tttcaatttc tggagaaagg cggacgccct ggaggagatc aacgagaaga
     4501 cccggaagat cctgaggatg gtccaattgc tggattccca ggatcgaccc gcgtcaatcc
     4561 tttcccacgg agagaggaag tacctcgaaa tcggcatcgc cttggccgcc gatcccaagc
     4621 tcctcctgct ggatgagcca acggcgggaa tgagtccggc ggagaccgtt ctggccatga
     4681 acctgattcg gaacctgcgg gatcagttga acctgaccat ccttctcgtg gagcacgaca
     4741 tgaacgtggt catgaacata tccgacgaag tcttctgctt aaacgaggga agagtattgg
     4801 cctgcgggac tccggaagcg atcagtgccg atgcctcggt ccagaaggtt tatttggggg
     4861 gaacgaaggc atgttagccg tcgaaaacat caatacctac tacggaacca gtcatattct
     4921 gcatgatgtg tccctggacg ttcaggacgg ggaaatcgtg gtccttctcg gccggaacgg
     4981 ggtcgggaag acaaccaccc tgaagacgat catggggatt cagccctgcc ggagcgggaa
     5041 ggtcaccttc aacggtcaga acattaccgg cttccccccc gatgagatcg cccgccgcgg
     5101 gatttccctg gttcccgaag accgacgggt cattcccaac ctgaccgtat tcgagaacct
     5161 gaaattggga atgctggtcc ggatgaaaag ggaaaaggtc gacctgcccg agacgctgga
     5221 gatggttttt cattattttc ctcgtctcaa ggagcgcctg aaccaattag gagaaagtct
     5281 ttcggggggc gagcagcaga tgcttacgac ggcccggggg ctggtagcga atcctaagct
     5341 catgatcatc gatgagccta cggaggggct ggcacccatc cttgtggaag aaatttggga
     5401 gatgctgaag aagctccatg agaataaagt gacgatcctt ctggttgaac agaattacga
     5461 gaagtccctc cagctgagca agaatttgcg ggcctacatt ctggagaaag gccacatccg
     5521 gatgagcgga aagcgcgggg aactgcacga gaggcaggaa gaagtggaaa aatatttggg
     5581 agtcaagttg tgaaaaaatg cccatagggc aaagttctta aaattgctta aggggatttt
     5641 tttctctaac ccctttcatg cacttcgaaa tctgggacca atcaaaagga ggctagaaat
     5701 gagcaaggac agcgtagtga agtatgatga gcgaggcgtt ttggacgggg gaacggtcgg
     5761 cctgccccgc aagatgaccc gggagatttg gctggaggaa gtctttcccg aatggggcac
     5821 gtacctgaat tatgaaattg atcagtttaa ggtcaagccc aaaaccgggg ccatgtggta
     5881 cttcggcggt ccgtcctttg cattgaagtc tcaggggggg gcggtcttcc tggtcgatct
     5941 ctatgccggc cccagcctct tcacggatta ttcctattgc ggggtctgcc gcacgagcgg
     6001 cgctgacaag ctttactgga tgcgcctcaa tccccacgtc atcgatccct ggaagttcaa
     6061 acgtctggat gccgtttgca tcacccatca ccatcaggac cacatggact tctataccat
     6121 tcaggcggct ttacagacga ccaaatgcaa gttcattgct cctccggaaa cctgtcgccg
     6181 gatgaagaag aacatgggcg tgcccgagga ccggatcatc gtcaccaagc ccggaaagag
     6241 cgtcaccatc gaggatatga aaattgactt cgctcccaat ttcgatgtta tcgtgaccaa
     6301 gacgggtttt gaaaccccgc agcctttcga agaggtggcc gtcagcttta ttttcaacac
     6361 ccaagggggc ggcatcgcct ttctgggaga tactctctat cacaacgggt atcgcatggt
     6421 gggagagaaa tacaagatcg atgtggtctg tatggacatg gggaataatg cccccgggta
     6481 taccgacaag atgtccccct gggacgtatg gcgggtggcc gaagcgatga atgcgaaggt
     6541 ggtcatcccc gaccatcacg acaactgggc caattgctac gaagacccca gttacctcgt
     6601 ggatattgtc aaccgcaaga ataaagagaa acgccccgag atgaagaccg tcatccttct
     6661 ccccggagcc cggtacgaac atcccaccga caagaatatg ggccgctacg tctatcccga
     6721 ctggcg
//
LOCUS       DASPRR010000428         8726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2036989, whole genome shotgun sequence.
ACCESSION   DASPRR010000428 DASPRR010000000
VERSION     DASPRR010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036989"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1116)
                     /locus_tag="VEL68_15585"
     CDS             complement(310..1116)
                     /locus_tag="VEL68_15585"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXZ37442.1"
                     /translation="MNEVIQFVTQHGYSLVFLFVLADQVGVPVPSSPMLLAAGVLAGQ
                     GWIIFLPSFFLGVTAALGSNLIWYWIGRSRGESVLSLLCRVSLNPDSCVRNTASFFSR
                     YGARSLLVAKFIPGLNTIAPPLAGIVRMPLGSFLIYDTLGAGIWVGALIGAGYAFSYE
                     IEALLGSLLKMGSLFGFLLLGGLAAFIGWKYFQRRKFLHQIQMARIGSEELKEKIDRG
                     EDLAIFDVRHPMEFHADPRVIRGAVYAPLERWREEDYSRLKDKEVILYCG"
     gene            complement(1133..3421)
                     /locus_tag="VEL68_15590"
     CDS             complement(1133..3421)
                     /locus_tag="VEL68_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXZ37443.1"
                     /translation="MNDGWDIFKIYRLIRKKNQYPHLSGLMDITFMEILEQRGVINRE
                     TLFQKALNHLKADGLPDTEENRQDYVEALTDAYFANSFTAADIESYINLARKRDKAQT
                     LSMVVNRDQATSMEVYKALREFCEIPMGEVYISPEEAIGIRVALISRFISNQLPFISL
                     AKNHITIRDMELILQRALWSRRRPGRLGGKAAGMILAYKILLPLLEKHDPELEESVRI
                     PETWYLNSGVFSEFLDRNNLYLFHTFKYKDREEIEKEFLHVEEKFKYATFAPEVIDDF
                     RKILEEIGEHPIIIRSSSLLEDSFGLAFSGKYLSVFLTNQGNLETRLTHFIRGLKRVF
                     TSTFGPDPILYRMDHGLLDFDERMAMVVQKVVGRRWGDYFFPFVSGVLFSRNVYAWNP
                     KIKKEEGLGRLVLGLGTRAVDRVGSDYPRMVPFSHPRLRPEVTAAQIKKYSQRQVDVL
                     NLKTGQMETVDFRTLKDKTPHPDFFYAVSVDRDGHLAPPMFKTQDLSGGESCLTFENF
                     LEKTPFVGLAKRILSQIESAYGRPVDIEFAWDENKFYMLQCRSLSTRKEIERVKIPEK
                     IPQDKVLFATQAGLSNSVVPNMEYIVYVNPRAYDLLPTFQEKMEIAGVVNVLNKELAE
                     KRYALMGPGRWGSNDINLGVKVTYANINKAKLLVEVAFAKEGYKPEVSYGTHFFQDLV
                     EADIVIVPLFPDDPGVVLNEPFLLNSENLLGRLAPEVKNCEKVVRVIHVPTVCGGDYL
                     QLYLDVDSQQGVGFFGPREEEG"
     gene            complement(3620..4867)
                     /locus_tag="VEL68_15595"
     CDS             complement(3620..4867)
                     /locus_tag="VEL68_15595"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXZ37444.1"
                     /translation="MPEFNPFKIAQAQLDVAAARLGLDPATHELLRWPQIEFKFTLPV
                     EMDDGKVRVFHGYRVQYNSARGPTKGGLRWHPEETIDTVRALAAWMTWKTAVVDIPLG
                     GGKGGVTCNPKEMSEREKQRLARAYMRAVARILSVFKDVPAPDVYTTPQIMAWMMDEY
                     EVLVGEHHPGVITGKPIPLGGSEGRGDATARGGVYALREAGKVLGIDLKGKTMAIQGF
                     GNAGQFAALLAHEILGLKLVAVSDSKGGIYNPNGIDPKQVIDHKLKKGSLQGFPGSQA
                     ITNEQLLEMDVTVLFPSALENVITDSNASKIKCRVSCELANGPTTPEADEILYANNIF
                     VIPDFLANAGGVTVSYFEQVQNAYNFYWPLDEVHRRLDEKMTRAFHGVYQMYQREKVN
                     MRQAAYLVSVARVAEACKLRGWV"
     gene            complement(5238..6641)
                     /locus_tag="VEL68_15600"
     CDS             complement(5238..6641)
                     /locus_tag="VEL68_15600"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ37445.1"
                     /translation="MSDVFRYFFEPRSVAVIGASKTPGKAGNEILVNMLANGYQGKVF
                     PINPGGEEILGFPSYRSVREVPEAVDLAVFIVPAEKTLEPLKECAEKKVPAAVIASGG
                     YAEVDEQGAGLQEEILKVARAAGMRIIGPNTSGLTSTPAKLTTTFFPLGKVRRGPISY
                     IAQTGNFATHTMRWINTAENFGVARVAGLGNKIDIEDSEVLEYLGKDPETKAVLMYVE
                     GFRDPRRFLEVAKRVTRKKPVIILKGGRTKAGAERAFSHTASLAGNDSILDGAFQQAG
                     IARVRRYADLVNVAKGIAFQALPPGRRVSSLAPSGALGVVVADACESAGLEIAHHSEA
                     SLKKLREISASWVTVSNPVDMSAITPILGPVEAYRKVTEILLEDEGVDAVVAILLASP
                     RTPAEAHRFLPELSARYPQKPVYVSFTGDKPAFDAARDYLEKHSIPVFSPVEDIFEML
                     PILCRCREFIAPSPLSG"
     gene            complement(6792..7799)
                     /locus_tag="VEL68_15605"
     CDS             complement(6792..7799)
                     /locus_tag="VEL68_15605"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ37446.1"
                     /translation="MAALKRQEPDRVPFCELGIDRALAEGLMGWPATKTAAGGSLAKN
                     PYTVEESKALAAFLGFDCIWYLLRAPTFAHQGVGQEGRGFMGEGMIKSEADLEKIKLP
                     DPTKEEFYREAEHFAKNKGDYALVFATRIGFFQTVLSMGTENFLVSLYTDRPLVEKIL
                     DLYFDWMTVVVERMCQIGYDIFWTTDDYAYNTGMFFSPAIFREIIAPRYRRVLNKCTI
                     PWFLHSDGNIMESMDILVDLGVAATHPNQKGAMDIREVKRRYGDRVCVIGNVDLNILG
                     MGTPEETDREVRELIRDLAPGGGYMISSGNSLASYLKPDCVLAMSRAIQKYGKYPIRI
                     S"
     gene            complement(7844..>8726)
                     /locus_tag="VEL68_15610"
     CDS             complement(7844..>8726)
                     /locus_tag="VEL68_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37447.1"
                     /translation="YDRIVSSFEEIEDDVIHSVAPIPAFPWLEAMLGIRAQVGNESIW
                     AKEGGFDYRDTGKLNLSRDNPWRKKYLEFVSALQDRYGDRYPVGQPILRGTSDMVAAL
                     RGTQQMIFDLYDHPQEFQRLARSCTDFFIGLVKDQLAVTKPFYGGYIVEQFTLWAPDG
                     IVRMQEDASALFSPALYVEFLQEEDRRIASAFPYDVIHLHSSSLHLLDRFVDIEPQKS
                     FEINKDQGGWGVPRMLPLFKMVQSRGKKLIIRGKLDLADLELLRKELSPRSLYLQIVI
                     EKPEEAKQFQDFFRPWS"
BASE COUNT         1797 a         2548 c         2459 g         1922 t
ORIGIN      
        1 acggttttac atcaaattac tgctgtacct agtgtggcgt ccccataaat tcagtgaata
       61 aatttatcag gaaggggggg gttcttcgcc ccgctccctg ctcgggggca aaattatgca
      121 gcttatttct gagacgcgac actagcgcta ataacctttt tgctatacgt taggctccat
      181 gccctttgca gttgtcaccg gatacccctt cgctttccat tccttgaacc cgccctcgag
      241 cgggcgtact cggaggattc ctctttggcg taactgcagc gccgcccggg cgctggaggc
      301 ctcctcgggt caaccgcagt aaaggatgac ttccttgtcc ttcagtcggg agtagtcctc
      361 ttccctccat cgctccaagg gagcgtagac agctccccga atcacccggg ggtccgcatg
      421 aaactccatg gggtgtcgca cgtcgaagat cgccaggtcc tccccccggt ctatcttttc
      481 cttcagctcc tcggacccga tccgcgccat ctgtatctgg tgcagaaatt tgcggcgctg
      541 aaaatatttc cagcctatga aggcggccag cccccccagt aaaaggaacc caaagaggga
      601 acccatcttc aagagcgacc cgaggagagc ttcgatctcg tagctgaagg catacccggc
      661 gccgatcagg gccccgaccc agatacccgc ccctaaagtg tcgtaaataa gaaaagaccc
      721 caggggcatc cgcacgatcc cagccagggg aggggcgatg gtgttgaggc cggggatgaa
      781 tttcgccacc aggagcgagc gggccccgta gcgggaaaaa aaggacgccg tgttgcgcac
      841 acaggagtcc ggattcaggg agacccggca gagcaaggac agtaccgatt ctcccctgga
      901 cctgccgatc cagtaccaaa tcagattact ccccagcgcc gcggtgaccc cgagaaaaaa
      961 cgacggcagg aaaattatcc atccctgtcc cgccagaact cctgccgcca aaagcatggg
     1021 ggaggacggc acaggtacgc cgacctgatc ggctagcacg aagagaaaca ccagggaata
     1081 gccgtgctgg gtgacgaact gaattacttc attcatggcg agaacagccc atctacccct
     1141 cttcctccct gggcccgaag aaaccgaccc cctgctggga gtccacgtcc aggtagagct
     1201 gaagataatc ccccccgcat actgtaggga cgtggatgac ccggacgact ttctcgcagt
     1261 tcttcacttc gggggccagc cggcctagaa ggttttcact gttcagcaaa aacggttcgt
     1321 tcaagaccac tccggggtca tccgggaaga gggggacgat cacgatgtcg gcttccacca
     1381 ggtcctggaa gaagtgggtt ccgtaggaga cctccggctt gtagccttcc ttggcgaagg
     1441 ccacctccac caggagcttg gccttgttga tgttggcata ggtgaccttg acccccaggt
     1501 tgatatcgtt gctcccccag cggccgggcc ccatcagggc gtagcgcttt tcggccagct
     1561 ccttgttgag cacgttcacc accccggcaa tctccatctt ttcctgaaag gtgggcagaa
     1621 ggtcgtaggc ccgggggttt acgtaaacga tgtattccat gttaggcacg acgctgttgg
     1681 aaagccccgc ctgggtggca aagagaacct tatcctgggg aattttttcg gggattttca
     1741 cccgctctat ttccttccgg gtggaaaggg aacggcactg aagcatgtag aatttgttct
     1801 catcccaggc gaactcgata tccacgggtc ggccgtaggc cgattcgatt tgggagagaa
     1861 tccttttggc cagtccaaca aaaggggtct tttccagaaa attctcgaag gtgagacaag
     1921 attccccccc gctcaggtcc tgggtcttga acatgggggg ggcgaggtgc ccgtcccgat
     1981 cgaccgaaac ggcgtagaag aagtcgggat ggggagtctt atctttcaat gttcggaaat
     2041 caaccgtctc catctgtccg gtcttcaggt tgagcacatc cacctgcctt tgggaatatt
     2101 tcttaatctg cgcagcggtg acctcgggac gcagccgggg atggctgaag gggaccatcc
     2161 gggggtagtc cgaacccacc cggtccacgg cccgcgttcc cagcccgaga accagccgcc
     2221 ccagcccttc ttccttttta atcttggggt tccaggcata gacgttgcgg gagaagagga
     2281 cccccgagac aaaggggaaa aaataatcgc cccacctgcg acccaccacc ttctgcacca
     2341 ccatggccat tcgttcgtca aagtccagga gtccgtggtc catgcggtag agaatggggt
     2401 cgggcccgaa ggtgctggtg aagacccgct tcagcccgcg gatgaaatgg gtcagccggg
     2461 tctccaggtt gccctggttg gttaggaaga cggagaggta cttaccggag aaagccaggc
     2521 cgaagctgtc ttccaggagg ctcgaggacc ggatgatgat gggatgctcc ccgatctcct
     2581 ccaggatctt gcgaaaatcg tcgatcacct cgggggcaaa agtggcatac ttgaactttt
     2641 cttccacatg gaggaattcc ttctcgatct cctcccggtc cttgtattta aaggtgtgaa
     2701 agaggtacag attgttccgg tccaggaatt cggagaaaac ccccgagttc aggtaccagg
     2761 tttcgggaat ccgcacggat tcttccagct cgggatcgtg tttctccaga aggggcagga
     2821 ggattttgta ggccaggatc attcccgctg ctttcccccc caaccgtccg ggccgacggc
     2881 ggctccacag agcccgttgc agaatgagct ccatatcccg gatggtaatg tgattcttgg
     2941 ccaggctgat gaaggggagc tggttggaga tgaaccggct gatgagggcc acccggattc
     3001 cgatggcctc ttccggcgag atgtacacct cccccatggg gatctcgcag aattccctca
     3061 aggccttgta gacttccatg gaggttgcct ggtcgcggtt gaccaccatg ctgagggtct
     3121 gcgctttgtc cctcttccgg gccaggttga tgtagctctc gatatccgcc gcggtgaagg
     3181 agttggcaaa ataggcgtcg gtcaaagctt ccacgtaatc ctgccggttt tcctcggtgt
     3241 cgggcaggcc gtcggccttc agatgattca gagccttctg aaagagggtt tcccggttga
     3301 tgactcccct ctgctccaga atctccatga aggtaatgtc catcagcccc gacagatggg
     3361 gatattggtt cttcttccgg atcaggcggt aaattttgaa gatatcccag ccgtcgttca
     3421 ttcgaatctc cttggggcag gagaattcat aaccgaatca tcttatgcct tttaactctg
     3481 attcaagcac caagtaccaa attccaatga cccaatttcc caaactcatt tcctatgtcc
     3541 attctccggt ttgaaatttt gaatatttgg attttactgt ttatttgggt tttggtgatt
     3601 ggtgcttggg atttctcttt tacacccatc ctcgcaattt gcacgcctcc gctacccttg
     3661 ccacggaaac caggtaggca gcctggcgca tgttgacctt ttcgcgctgg tacatctggt
     3721 agactccgtg gaaggcccgg gtcatctttt catccagacg ccggtgaacc tcgtccaggg
     3781 gccagtagaa attgtacgca ttctgaacct gttcgaagta cgagacggtc acccctcccg
     3841 cgttggccag gaagtcaggg atgacaaaga tgttattggc atagaggatt tcgtccgctt
     3901 ctggggtggt gggtccgttg gccaactcgc aggagacccg gcatttgatc ttcgaagcat
     3961 tcgaatcggt gatcacgttc tccagagcgg aggggaatag gaccgtcacg tccatctcca
     4021 ggagttgctc attggtgatg gcctgcgatc ccggaaatcc ctgaaggctt ccctttttca
     4081 gcttatgatc gattacctgc ttggggtcta ttccattggg gttatagatc cctcccttgg
     4141 aatcggagac agccacgagt ttcaacccca gaatctcatg ggccaggagg gcggcgaact
     4201 gccccgcgtt gccgaatccc tggatggcca tggtcttgcc cttcaggtcg atgcccagga
     4261 ctttgccagc ttcgcggagg gcgtatactc ccccccgggc ggtggcatcc ccccggcctt
     4321 ccgaacctcc cagggggatg ggcttaccgg tgatgacccc ggggtggtgc tctcccacca
     4381 ggacctcata ttcatccatc atccaggcca tgatttgagg agtcgtgtat acatcggggg
     4441 ccggaacatc tttgaagacg gagaggatac gcgctaccgc tcgcatgtag gcccgggcca
     4501 ggcgctgctt ttcccgctcg gacatctcct tgggattgca ggtaacgccc cccttccctc
     4561 cgcccagggg tatgtccacc accgcagtct tccaggtcat ccaggcggcc agggcccgca
     4621 ccgtgtcgat ggtctcttcg ggatgccacc gaaggccgcc cttcgtcggg ccccgggcgg
     4681 agttatactg gacgcggtag ccgtgaaaaa cccggacctt cccgtcgtcc atttccaccg
     4741 gtagagtaaa tttgaactct atttgaggcc agcggagcag ctcgtgcgtc gccgggtcga
     4801 gacccaatcg ggccgcggcc acatccagtt gcgcctgggc aatcttaaaa ggattaaatt
     4861 ccggcatgga aaatcctccc tttccttttc agttcccgcc ctggaaagcg acgtcgacct
     4921 gactgtcggc atcgcattcc cttgagatca gtataaattc ctccccggtt attttcaata
     4981 gaatctgatc ccttgacttc tgagattccc tgtttagggc ccgtaagccc agaggaaatc
     5041 tcgcagacag aatcggcggg actgggttct cttacagagg atatcctgac atcctcaaag
     5101 aagggcggga atccactgaa gtcgccaaca gggctgaaga aaaactgccc gccccgaccg
     5161 atggaattgt tggcgcctca ttatgtctgc gagatttcct ctgggctttc gggccgggct
     5221 gggtagattg aatggtctca tcctgaaagc ggggaagggg cgatgaattc tcggcaccgg
     5281 cacagaattg gaagcatttc gaaaatatct tctaccgggg aaaaaacagg aatggaatgc
     5341 ttctccaggt aatctctcgc cgcgtcgaag gccggtttgt ctccggtaaa gctcacatag
     5401 accggcttct gggggtatct cgcagaaagt tcgggaagaa accggtgggc ttctgccggg
     5461 gtgcggggag aagcaaggag aatggccacg accgcgtcca ctccctcatc ctcgaggaga
     5521 atctccgtga ccttgcggta ggcctccacc ggacccagga tgggagtgat ggccgacatg
     5581 tccacagggt tggagacggt cacccaggag gcactgatct ccctgagctt tttcaggctg
     5641 gcttccgagt ggtgggcgat ctcaaggccc gcggattcgc aggcatcggc caccacaacc
     5701 cccagcgctc ccgaaggggc aagagaagaa actcttcttc ccgggggaag agcctggaag
     5761 gcgattccct tggccacatt gaccaggtcg gcgtaccgcc tcacccgggc gatgcccgcc
     5821 tgttggaaag ccccatcaag gatggaatcg ttcccggcca gagaagcggt gtgggaaaag
     5881 gccctctccg ctcccgcttt tgtccgccct cctttgagaa taatgaccgg ctttttccgg
     5941 gtcaccctct tggccacttc caggaatcgg cgggggtcac gaaacccttc cacgtacatc
     6001 aagacagcct tggtctccgg gtcctttccc aggtattcca gaacctccga gtcctcaata
     6061 tcgatcttgt tccccagtcc cgctacccgg gccacgccga agttctctgc cgtgttgatc
     6121 caccgcatgg tatgggtggc gaagttcccg gtctgggcga tgtagctgat aggcccccgc
     6181 cgcacttttc ccaaggggaa aaaggtggtg gtcagcttgg caggggtgct ggtcaggccg
     6241 ctggtgttgg ggccgatgat ccgcatcccg gccgcccggg ccactttaag gatctcctcc
     6301 tgcaggccgg ccccctgctc gtccacctcc gcatatcctc cggaggcaat gaccgcggca
     6361 gggacctttt tctcggcgca ttccttgaga ggctccagcg tcttttccgc tgggacgatg
     6421 aataccgcca ggtccaccgc ctccgggacc tccctcaccg aacgatagga tggaaatccc
     6481 agaatttctt cccctccggg gttgatggga aagaccttac cctgataacc gttggccagc
     6541 atgttgacca agatttcatt ccccgctttc cccggggttt tcgaagcacc gatgaccgcc
     6601 acgctgcgcg gctcgaagaa gtagcggaat acgtcggaca tgacgatctc ctgttcagtt
     6661 caggcttatt ggggcgatca tccaagaggc gtgcggggtc gcccgtgcgg agttcggact
     6721 cctatacctt ggtagaaaag ccgtgattgt ctagaagcaa ggaccggaga tatctccaag
     6781 tgaaaacccg actagcttat tcgaattgga tactttccgt acttttgaat ggcccggctc
     6841 atggccagga cgcaatccgg cttgaggtag gaagccaggg aatttccgct gctgatcatg
     6901 taacctcccc ccggggccag gtcgcggatt aattctctca cttcccgatc cgtctcctcc
     6961 ggtgttccca tccccaggat gttgaggtcc acatttccga tcacgcagac ccgatctccg
     7021 tacctccgct tgacctcccg gatgtccata gcgcccttct ggttgggatg ggtcgcggcc
     7081 acgcccaggt ccacgaggat gtccatggat tccatgatat tcccgtcgct gtgaaggaac
     7141 caggggatgg tgcatttatt caggacacgc cggtagcggg gggcaatgat ttcccggaag
     7201 atggccgggg agaaaaacat gcccgtattg taagcgtagt catcggtagt ccagaaaatg
     7261 tcgtagccga tctggcacat tctctccact accacagtca tccagtcgaa atagaggtcc
     7321 aggatcttct ccaccagcgg gcggtcggtg tagagggaca cgaggaaatt ctccgtgccc
     7381 atgctcagga ccgtctgaaa aaagccgatg cgggtggcaa agaccagcgc ataatcgcct
     7441 ttgttcttcg cgaagtgctc cgcctcgcgg tagaattcct ctttggtggg gtcgggcagt
     7501 ttgatctttt ccaggtccgc ctcggacttg atcattcctt cccccatgaa gccccggcct
     7561 tcctggccca ccccttgatg ggcgaaggtc ggggcccgca gaaggtacca gatgcagtca
     7621 aagcccagga aggcggccag ggctttggat tcctccacgg tgtaggggtt cttggccagg
     7681 ctccccccgg cagcggtctt ggttgccggc catcccatga gcccctccgc cagggcgcgg
     7741 tcgataccaa gctcgcagaa ggggacccgg tcgggttcct gccgcttcag agccgccatg
     7801 attctctctc tgggagtcat ttccattgat ccctcgctgt ggatcagctc caagggcgga
     7861 aaaaatcctg gaactgtttc gcttcttcgg gcttctcgat cacgatctga aggtaaaggc
     7921 tgcggggaga aagctccttg cgaagcagtt ccaggtccgc caggtccaac ttcccccgaa
     7981 tgatgagttt cttccccctt gactggacca ttttgaaaag agggagcatg cggggcactc
     8041 cccatccccc ctggtctttg ttgatctcga aggacttctg tggctcgatg tccacaaacc
     8101 ggtccagcaa atgaagcgaa gaggaatgga ggtggatgac gtcataaggg aaggcggagg
     8161 cgatccgccg gtcttcttct tggaggaatt ctacataaag ggcgggggag aagagggccg
     8221 acgcatcctc ctgcattcgg acgatcccgt caggagccca gagggtaaat tgttccacta
     8281 tatagccccc atagaacggt ttcgtgacgg ccagctggtc cttcaccagg ccgatgaaaa
     8341 agtccgtgca ggacctggcc aggcgctgaa attcctgcgg atgatcgtag agatcaaaaa
     8401 tcatctgctg cgtgccccgc agcgcagcca ccatgtccga ggttccccgg aggatgggct
     8461 gccccaccgg gtatcgatcc ccgtatcgat cctgcagagc ggaaacgaac tccaaatatt
     8521 ttttcctcca ggggttatcc ctcgagagat tgagcttgcc cgtgtcccga tagtcaaagc
     8581 ccccctcttt ggcccagatg gactcgttgc ccacctgggc ccggatcccc agcatggcct
     8641 ccagccaggg aaaggctggg atgggggcta ctgaatggat gacatcgtct tcgatttcct
     8701 cgaaggagga cacgatgcgg tcatag
//
LOCUS       DASPRR010000429        32548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_910860, whole genome shotgun sequence.
ACCESSION   DASPRR010000429 DASPRR010000000
VERSION     DASPRR010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 32548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32548)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32548
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910860"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VEL68_15615"
     CDS             complement(<1..274)
                     /locus_tag="VEL68_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HXZ37448.1"
                     /translation="MLKQSPAHKFPSDLKKFKYEIEDHARGYDLDFFPVIFEVLDYQE
                     INEIAAYQGFPSRYPHWSFGMQFEEVSKSYAYGLSKIYEMVINNDPC"
     gene            complement(307..723)
                     /locus_tag="VEL68_15620"
     CDS             complement(307..723)
                     /locus_tag="VEL68_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXZ37449.1"
                     /translation="MEPRRVQKDFIKTPGGPLSEELTEKIISTAMEVHSQLGPGLLEN
                     VYEEAMAHEFALRKIPFVRQKEIKLDYKGKDIGWHRVDFLVGNEVLVELKSVEALHGI
                     HEAQMMACLRALQAREGLLINFKVERLEDGIRRIVI"
     gene            complement(728..1831)
                     /locus_tag="VEL68_15625"
     CDS             complement(728..1831)
                     /locus_tag="VEL68_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HXZ37450.1"
                     /translation="MYQSKRIDRDRARFKKIVHGAIRSGIRKYLSHGEMIGRKGKDLV
                     SIPLPQLEIPRFRFGQAQKGVGQGEGNPGDPLALDPEGQGKREAGSDPTEHILEVELT
                     LEELASILGEELSLPRIEPRGKKNIVTEKDKYHSIARTGPEALRHFKRTFRQALRRQV
                     ISGTYDPEEPRVWPFKEDKLYRSWKALPEQESNAVVIYMMDVSGSMGDEQKEWVRLIS
                     FWIDTWLTSQYRGVERRFIIHDAEAREVDSETFFHTRESGGTKISSAYELCLKLIQRS
                     FPPEDYNLYPFHFSDGDNFGDDDERALKFLAEILSLANLFCYGQVETLYGSGSFMKFI
                     QERIDGDEKMCIARIRDRESIFDALRSFLGTGR"
     gene            complement(1943..4000)
                     /locus_tag="VEL68_15630"
     CDS             complement(1943..4000)
                     /locus_tag="VEL68_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HXZ37451.1"
                     /translation="MLPQQEIRSLIDRYTQDSSYQALHWEGTFEDYLKILGDRPEILR
                     NSFQRVYDLIISYGTEEYLENKDKLFRYRFFSDPVNGGLEAVFGLERPLMHLVNVFKS
                     ASRHYGTERRILLLHGPVGSAKSTIVRLLKKGLEVYSRKPEGALYTFSWRLNGPGQGE
                     TGEPCPMNEEPLHLLPLAVRGPLIDGLNAKINGQRKMVVDGDLCPSCRFYFRTLMQKF
                     AGDLKKVLDHVRVFRLILSEQDRIGVGTFQPKDEKNQDSTELTGDINYRKIAVYGSDS
                     DPRAFNFDGEFNVANRGLIEFVEVLKLDVAFLYDLLCASQEHVIKPKKFPQTHIDEVI
                     IGHTNEPEYRKLQNNEFMEALRDRTVKVDIPYITRLSDEIKVYEKDYGPNRVQGKHIA
                     PHTIEMAAMWAVLTRLKEAKKANLTLLQKLKLYNGKTLPGFTEENIKELKEEAEREGM
                     EGISPRYIQDKISNALVSEEAEGCVNPFMILNELESGLDHHSLITSEEQKKRFRELMI
                     VVKDEYADMAKNEVQRAICADEEAIKRLCSNYIDNVKAYTQKEKVKNKYTGESEEPNE
                     QLMRSLEEKIEIPESRKDDFRREVMNYIGALAVEGKSFDYRSNERLHRALELKLFEDQ
                     KDTIKLTSLVSNVVDKETQEKIDVVKSRLIKNYGYCEVCATDVLHFVASIFARGDLKE
                     QNK"
     gene            4647..5216
                     /locus_tag="VEL68_15635"
     CDS             4647..5216
                     /locus_tag="VEL68_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXZ37452.1"
                     /translation="MNSREKVFFESFREVVKAIHSTLDIRQVLQMLATKVSQVMNLKG
                     CAIRLLDPTKRTLELVAAHGLSEKYISKGAVAADRSIAEAMEGKTVSIYNATKDPRAQ
                     YKKEAEEEGIYSIVSVPFSIKGRIIGVLRLYTSQPRNFSQEEIGFFEALAEMGALAIE
                     NARMYEQLRKDYENLMSDILMFVGYRRSI"
     gene            complement(5273..5710)
                     /locus_tag="VEL68_15640"
     CDS             complement(5273..5710)
                     /locus_tag="VEL68_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ37453.1"
                     /translation="MLLALCSLLFATGCSFPRVIILKDPLTPEEHLNLGVTYEQQGDF
                     DNAIKEYNLAAKNLPGALLYLGNAHFQKKEWKKAEDYYRLAIEKEPDNADAYNNLAWL
                     YYTRREKLDEAETLAQKALRLNPGKGDIYRDTLEKIQEAKRGS"
     gene            complement(5728..6189)
                     /locus_tag="VEL68_15645"
     CDS             complement(5728..6189)
                     /locus_tag="VEL68_15645"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HXZ37454.1"
                     /translation="MLSLKTARAIEGVPFFPQEQFQCGPASLAGVLNYYGLRITPAEI
                     AAEVFSRSARGTLDMDMVFYAQRKGMKAEQYAGSLEDLQGSIDSRRPLIVLIDQGFWV
                     YQNHHFMVVVGYDKGGIVVNSGKEENKFISLDSFLKTWEKTKFWTLRITPP"
     gene            complement(6329..6727)
                     /locus_tag="VEL68_15650"
     CDS             complement(6329..6727)
                     /locus_tag="VEL68_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HXZ37455.1"
                     /translation="MARFLNRWIAWYLVVAMFVIGITPRVYAGFAPSEVVGLSPGERA
                     ADLQKVRQFLEMKMVGERLKELGYTSEEIQSRLSQLNDEQIHQLATKLDDLKVAGDSA
                     LGIIIALLIIAILVIVIIQLLGHKIVIKKE"
     gene            7128..8057
                     /locus_tag="VEL68_15655"
     CDS             7128..8057
                     /locus_tag="VEL68_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ37456.1"
                     /translation="MTSKEFLTTGEAAKLVNISRSTISRNFDKGIFQGKKNPITGERL
                     ISRESLINFMRQYDLPVDTLELGKKRILVGSSDDQLFSLLQRTLSEDDRLELERVVFG
                     GDVLIWCSKENPDLLVIDEELPDISSMEIIRSLRRTDNQQALKIICAGKARSVRHCLD
                     WGADEVLGKESLSREELAKTIYAFLNLPQEIPEEERQYSHQRRWPRMALHLPLKIGVY
                     SVRTPYRRDTGLATMENISLGGAFLSEFSLEGGMIPCEPFRILLEADQEPLKDWKAHG
                     KVVRLQSNGTVSTGVQFTRLPKASLKMIEALAQ"
     gene            8222..9292
                     /locus_tag="VEL68_15660"
     CDS             8222..9292
                     /locus_tag="VEL68_15660"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ37457.1"
                     /translation="MSSSITKDESQMSRREFLKAASVGAAGLALMGLPSGRSEAVEKY
                     PHWGEAVVIRDESATFGPNIDSKVVREMLNEAVKALTNGGGWPSLFSSYKTGETVGIK
                     VNCQWPRVPTQPKVVAAIVNELVKMEIPKDKIIIWDAEAGALGYSEMWNVSMEFRGVR
                     TAATDKLEDPFDASKTMDMQGNPAQLSRLLTGPAHLINVPVLKNHFEAGITFALKNHV
                     GSVGKDNKSRQILFHHPMDEKNPIHKMAGRGAPISERIALINTAPDIKNKTRLIVGDA
                     LFGVFTRGPLGEPEFAYNGLIVGTDPVATDHQARLIIEGERKKSNLPFVPSLHIDHAI
                     KLDLGAPLEEMKVTPIHRTKKG"
     gene            9327..9866
                     /locus_tag="VEL68_15665"
     CDS             9327..9866
                     /locus_tag="VEL68_15665"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HXZ37458.1"
                     /translation="MILIFLILSAGSACADQASASPEKLLAAMDLLPLDDDLPGWKRS
                     EKLLSASNEEELYKILNGGATLYIQHGFQVFVGQSYRGPKGVESEVYIFDQGTAQNAR
                     GLYENPLAKPGRSKEVPDLGEEARIDTTPLFSYGVDFVQKGFFVRVIIQEKTEEGLKV
                     ALTFAHNISKRIKNRMSRR"
     gene            complement(10038..10820)
                     /locus_tag="VEL68_15670"
     CDS             complement(10038..10820)
                     /locus_tag="VEL68_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37459.1"
                     /translation="MSQSEKEGLYRLLIPPSLFKRFQINPLTFTDYEGNRLVRFYCPE
                     REETVMIEVKRKLDDPDPIYSIQASDGPDFTQVNWDFLIVNDPDSDRFNIDVDEEGRD
                     TLWGRGSRNLPEEIKALRSGMSPGQVRKGLGMTREVITGLEYFARILDIKTISLEALF
                     YHNAIVYERCGFTYFEGFKRMTRIHQAFQPGGKLFRRLDGSTPFRQLGFEKTVRGRSW
                     AIHDGVVSEIDDDVLDEGWYSPKMYLLVGQPRTTTTFPDAVY"
     gene            complement(10931..11977)
                     /locus_tag="VEL68_15675"
     CDS             complement(10931..11977)
                     /locus_tag="VEL68_15675"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ37460.1"
                     /translation="MRSIFIGSTGSGPGQTLATWALAVRLKAKGLKVGFFKPYGLLPE
                     SPISPPGSSCDPDVLLFKQVLGVSEPDETLCPISLPGNQAFAGIQGEELMGKIQKAFQ
                     EVSRDKDVILIMGGKEIFFGEGGPGLSDGALVKSFDSFVLLVDRYQRDNLTFYSVLSL
                     NSFLDGRVKSAILNHVAPDKVDHVKTKVVSFLQQKGVKSAMAVPEDPILAALTVSSIA
                     DITGGEILCCLQGENNLVQTSTIGAKYLEGPFSLFKQVYNKVILVGLNPDEKPVTGII
                     LTGGKNPSEGVVRVAQDRGIPLILTRADTFQVMDRLEKAKPALTSKDEFKAHRFLKLI
                     DEINGGSKWVEELL"
     gene            complement(12152..12694)
                     /locus_tag="VEL68_15680"
     CDS             complement(12152..12694)
                     /locus_tag="VEL68_15680"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HXZ37461.1"
                     /translation="MKIPAIPKLSKRTWAFIVAILLILAAIPALGIVRFTTSHPFFCL
                     TCHKNQDIPERWLPSRMHPASVDCVDCHISRGGTPFSHSFSASDDLMSQRCAGCHPNI
                     PGGEQMDLDKVRVVKVSHKQHADKKVLCIDCHRNLAHDKGTPRTNRPTMETCYQCHQA
                     HPRTQACDKCHPINLVYTKK"
     gene            complement(12785..15013)
                     /locus_tag="VEL68_15685"
     CDS             complement(12785..15013)
                     /locus_tag="VEL68_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ37462.1"
                     /translation="MKFTRRDFLKVGFTSATVAGLGGSLAGCFSPPREVPRKVTRTSG
                     KAASVASTCLLCPAGCGIVGEVLDGRLEKMVGNPKHPNNRGKLCSRGHAGINTLYDPD
                     RLLQPMKRSGGRGEGKWTRITWDQALDEMAKRMGALKKSGRTEALWMEMNTPGSQDLM
                     ALEFLKAFGRPIVFSDSEWMNPNEKVGQMMTWGADTMVCDAARSRFILNFGSNPYENH
                     DLYIPLIQRIIEGRMTNAAKLVTFDVRLSNTAGKSNEWFPIKPGTDGIVALAMAQHII
                     QQGLHDKEFLSRWTNVPLSKLVEQLSSYTPEEAEKVSGVKAVDIMRLAKELAQAKPAA
                     ILFGRGVSGHQNGVMNTRCIALLSAVVGSADVPGGCCLPRKMDLGEPKGKGPFNNSFQ
                     ALTALKEGKGQPDFYFCYQANPAYATPPSAEVEKLLKDEKRIPFLVVADTHLTETGAL
                     ADLLLPMATYLESWNLESRPAMGLVPFVSLRQPVVLPLGKSLSLGDALSSLAKRLGDD
                     MVKAFPYGHSEEFMEKAAARIEGLAKAGGLAVLKKEGVWLDPAAKPEYRSYEKKGFPT
                     PSGKVEIYSARLQEKGFPPLPTYVPVKNHQETKETDFFLTVYRPNVMTLRLANSKWLA
                     EILHASPLWVNLRTAQAMGLKNGDRVKITSSAGSLTVPIRMTHGIHPKVVALPEGLGH
                     TALGKIARAIKYKSSDFDTNELWWEQEGNGANPNSVIPAQPDPAAGGTAWNDTLVTIT
                     KI"
     gene            complement(15031..15927)
                     /gene="nrfD"
                     /locus_tag="VEL68_15690"
     CDS             complement(15031..15927)
                     /gene="nrfD"
                     /locus_tag="VEL68_15690"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXZ37463.1"
                     /translation="MDAVILYNVFPQETFGALIAVYFYLTGLSAGSFVLSTMAFVFGM
                     EKFKPIGKIGVILATLFLVMAPLALLVHVGQPFKSWHLFVHLNITSPITWGSFLLTLY
                     PINCIIYGYFMFKGNKPLTRLFGTIGIPLAIFVHGYTGFILALGKARALWNTALMPFL
                     FLISAMVSGIALMILISMVKDKFFSREKTINRELVFGLGKMLAAMILIDLFLVLSDVL
                     VLLVSHVEAQEVAHLILTGKFSIYFIWVENVMGKIIPAIILLVPRFRNLATITIASIL
                     VVVGIFFMRYVVVLGGEFLPLV"
     gene            complement(16177..16500)
                     /gene="atpE"
                     /locus_tag="VEL68_15695"
     CDS             complement(16177..16500)
                     /gene="atpE"
                     /locus_tag="VEL68_15695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HXZ37464.1"
                     /translation="MRRKGLGILALTLVLILAFASLAFAAEEALGQEAKKFIGLACGL
                     AIAIAALGGGLGQGIAISKGLEGIARNPEAQPKIFIPMIVGLALIESLVIYALVISIL
                     LYAKL"
     gene            complement(16642..17358)
                     /gene="atpB"
                     /locus_tag="VEL68_15700"
     CDS             complement(16642..17358)
                     /gene="atpB"
                     /locus_tag="VEL68_15700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXZ37465.1"
                     /translation="MAGHAPFTWFHYIPGIGSLPHQVSMALFVLGLLCILAYAGFKKI
                     ASGPAGEALVPSDRLTVRNIFELFTEMVLKFLDDIIGHRGREFLPLIGTLGFFIFFSN
                     FLGLVPGFLPPTDNLNTTLACALVVFFATHYYGLKTHGIKYLRHFLGPVLWLSPLMLP
                     IEIISHLARVLSLSMRLFGNIMADHMLLSLTLAAPLILPLFLSPMSLFLGVFVSLIQT
                     FIFMLLSMIYISLAVEESEH"
     gene            complement(17358..17738)
                     /locus_tag="VEL68_15705"
     CDS             complement(17358..17738)
                     /locus_tag="VEL68_15705"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HXZ37466.1"
                     /translation="MSNHQEAQKLFSIEKRSAQILAVLLLASLWFQSWAVSLGLILGG
                     GIALLNFRWLWRILSQYLMAKKKYSLFQLIGKFFFLLLVLFLVIRFVRVNPVALVVGV
                     STLVLGMVFEVVRQSLRSNRKGVG"
     gene            complement(17749..17979)
                     /locus_tag="VEL68_15710"
     CDS             complement(17749..17979)
                     /locus_tag="VEL68_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HXZ37467.1"
                     /translation="MEEDKKRFYRQFARFSAIGLEMGLSVAIGLAIGYYLDRYFQTKP
                     WLTIIFLLLGVAAGFRRLFSLAKEIDKRERKK"
     gene            complement(18469..18945)
                     /locus_tag="VEL68_15715"
     CDS             complement(18469..18945)
                     /locus_tag="VEL68_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXZ37468.1"
                     /translation="MKNEKLSPKDKRLLGQIQGDLPLSLTPFVQVARNAGWKEKVLLR
                     RVRGFVRGGIIRRFGAILRHQKAGYEGNAMVVWKVPEEQVPRVSQTIVSFPPVSHCYL
                     RPPFPDWPFNLYTMVHGKGGKDCRRIAQAISKKTGIKDYRLLFSKREHKKSSMTYF"
     gene            19386..19742
                     /gene="ndhC"
                     /locus_tag="VEL68_15720"
     CDS             19386..19742
                     /gene="ndhC"
                     /locus_tag="VEL68_15720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXZ37469.1"
                     /translation="MLTDYLPIFIFFLVALGFALFTIAASAFLGRRKITPQKMMPYEC
                     GMDPVGQARNPFSVKFYIIAMLFIIFDIEAVFIYPWAVIFRDLKIFGLVEMAVFVAIL
                     LVGFIYVWKKGALEWE"
     gene            19733..20209
                     /locus_tag="VEL68_15725"
     CDS             19733..20209
                     /locus_tag="VEL68_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXZ37470.1"
                     /translation="MGIETQLGNNVLTASLEKLVNWARRSSLWPAGFGLACCALEMIV
                     AAAARYDMARFGMEVFRPSPRQADLMIVAGTVTKKMLPAVIRIYEQMPEPKWVIAMGA
                     CASTGGIFDTYSTVQGIDQYLPVDVYIPGCPPRPEALLYGIIKLHEKIGREKLLER"
     gene            20216..20671
                     /locus_tag="VEL68_15730"
     CDS             20216..20671
                     /locus_tag="VEL68_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXZ37471.1"
                     /translation="MDLTAVVDRIKKEIPQSILGVSAFREETTLEIRAEDLLSLCRFL
                     HDDPEMAFDFLIDLCGVDNLPREPRFQVVYHLCSTKTRLRLRLKVSLPGKNPKIASVF
                     PVWKAADWLEREAFDMYGITFTGHPDLRRILLTPDWQGHPLRKDYPLRG"
     gene            20703..21875
                     /gene="nuoD"
                     /locus_tag="VEL68_15735"
     CDS             20703..21875
                     /gene="nuoD"
                     /locus_tag="VEL68_15735"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HXZ37472.1"
                     /translation="MGDNSTMILNMGPQHPSTHGVLRVILELDGEVVVKATPDIGFLH
                     RGLEKLAESKTYHQCIPLTDRLDYVAGMSNNLAYVLAVEKLLGLEVPPRAQALRVLLS
                     ELQRISSHLLWLGTHAHDIGAMTPLFYAMRERDEILFLFERVCGSRLTPSYLRVGGLA
                     QDLPEGFEEKCREFTQTFNAHVDEYETLLTRNQIWMNRTQGVGVISTEEAINWGLTGP
                     NLRASNGNWDLRKAFPYSGYEKYEFVVPTGKNGDVYDRYMVRMMEMRQSNEIVRQVLD
                     KMPGGPVIAQDPKVIYPPKDRVQESIESLIHHFHLASAGFPAPAGEVYASIESPKGEL
                     GFYLVSDGTNRPWRFRVRPPSFVNLSALAVMVQGRLISDVVAVIGSIDIVLAEIDR"
     gene            21896..22240
                     /locus_tag="VEL68_15740"
     CDS             21896..22240
                     /locus_tag="VEL68_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HXZ37473.1"
                     /translation="MTLREAAEKLGLKPVAGLSRLSREFGQGYVSDLLSDVMANAKPG
                     DIWVTLQIHQNIIAVATLKELAGIILISGRQPEKQTLAKAEEEQVPLLLSDLPAFEVV
                     GQLYQMGITGRR"
     gene            22254..22799
                     /locus_tag="VEL68_15745"
     CDS             22254..22799
                     /locus_tag="VEL68_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ37474.1"
                     /translation="MQDLSLHILDIVENSLAAGARRVEIRVEEDMAADRMVIEIVDDG
                     CGMDEERARKALDPFFTTKTVRRVGLGLPLLSEACRMSNGEFRLESCPGKGTRVRAAF
                     QHSHIDRKPLGNMRDTLITLMSGHPQLDLLYEHRKEGKTFSLDTREIKADLGEIPLDA
                     PAVVSALRTLIQSGLEDLGAF"
     gene            22867..23250
                     /locus_tag="VEL68_15750"
     CDS             22867..23250
                     /locus_tag="VEL68_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXZ37475.1"
                     /translation="MAKLSIEDLKKIKESVRAAVTLREGGYRARVNVHMGTCGLAAGA
                     RAIMTAVLDEIQKRDTKDVVVVTSGCAGLCNREPMITVERMGEPPVKYVDLTPDKMRQ
                     IFSEHVLEGKIVEKYALAIGSETTF"
     gene            23395..25188
                     /locus_tag="VEL68_15755"
     CDS             23395..25188
                     /locus_tag="VEL68_15755"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXZ37476.1"
                     /translation="MAAKVFRSHILMCAGTACVSCGSFKVRERLEKELKARGLDEEVK
                     VVITGCNGFCAEGPIMVVYPEGIFYGHVTPEDMPLVVEEHIMKGRPVPKLFYKEPVKK
                     EAIPRMRDIPFFGLQVLRVLRNRGLIDPEAIDEYIAKDGYVALHQALTQMTPGQIIAE
                     VKESGLRGRGGAGFPTGDKWEECRKYDRFPKYVICNGDEGDPGAFMDRSVLEADPHAV
                     LEGMAICAKAVDSHEGYLYVRAEYPLAIKRVNRAIEQARNYGLMGKDIFGTGFDFDAF
                     VYPGAGAFVCGESTALMFSLEGKRGMPRAKPPRSAEAGLWGQPTVLNNVETFANIPQI
                     MLHGAKWFNSLGTEKSKGTKVFSLAGCVNNIGLVEVPMGISLRSIVFDIGDGIKGSNK
                     FKAAQLGGPSGGFVPENLLDTPVDYESLEATGAIMGSGGIVILDEAACMVDSARFFTD
                     FCVDESCGKCVPCRTGLAVMLRKLEDIVGGKGQEGDVEFLEDLGQHIKRMSHCGLGQS
                     APNPVLSTIRHFRHEYEEHIRDKRCRSLVCRALIRFRVNEEKCKMCGLCLKACPVGAV
                     TWERKKPAKINLDLCTKCKSCIRACKFWAIE"
     gene            25246..27906
                     /locus_tag="VEL68_15760"
     CDS             25246..27906
                     /locus_tag="VEL68_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ37477.1"
                     /translation="MAKEQKMVTLTIDGVQVSVPPGTMIVEAAAKAGIKIPTLCNNKR
                     LLPYGACRMCVVQQKGRRGFLMGCFNPVRNAMEILTNTPEIIKARRIQLQLILISHPL
                     DCPVCDAGGWCDLQNLAYEYGVAENPFKGKKTDLPVDHVSPFIERYPNRCILCGMCVR
                     ICDEVVGASELSFVNRGMKTRITTDLDRPLNCEFCGQCVSVCPVGALNDRILLHKARV
                     WDLKDTLTVCAYCGVGCTLLVGTRDNKVMRVRADEDLGLNQGNLCVKGRFGWEYIHNP
                     KRLTAPLIRKNGEPVKATWEDALGLAARRLKEIKAEKGGSALAGLASPRLTNEELYLF
                     QKFCRGVLGTNHLDHAGGYSYAAHLALRNSLGYAASTNSINEVRKADVILLLRSDLSE
                     THPVIKSEVVLAAKRRKAKVIIVNSRNIYLKKFSTLSLTVTPGNEVALVNGMMAVILK
                     EGLTQEDFLQSRTQGLAALKKNLESFSLQKVEAQTGVAADSLAEAARLYAKAKTGVIL
                     ISTGQNSGQEDSALAQAAANLALLTGKIGRENCGVFVLGEKNNSQGALDMGVTPGLLP
                     GYADLRDTAERSRFEKAWNLTLPDQEGRGALAILQGIEEGKIKGLYLAGENPAVTYPD
                     SERTRKALASLDFLVVQDCFLTETASLAHVVLPAVTFAEKEGTFTNVDRRVQRVRPAL
                     AALGEARSDLSIFQGLAQEMGTPLGSGSAQEVNDEMRSLVTLYGGIGFTRLDSPGVSA
                     GIQWPCPSADHPGTPIVYEKEFPVEKARLVFTDYQEAKKETDSSFALATGPTMFHSGS
                     LSTLSPGLVRLQGDGFVLVHPTDAKNLGLTEGQKVTLESPQGRVMVKVSISPRTGPGV
                     LFVPTHFAENGGNRLTGWDLKTARVKLEKS"
     gene            27935..28921
                     /gene="nuoH"
                     /locus_tag="VEL68_15765"
     CDS             27935..28921
                     /gene="nuoH"
                     /locus_tag="VEL68_15765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXZ37478.1"
                     /translation="MLIYLLISVVKVLIVFTVMLLIVAYMTLMERKVLGHMQVRFGPN
                     RVGPFGLLQPVADGIKLFFKEDIIVPFANRIIYILAPAVIVITALVSFAVIPFGNSIK
                     IMGQSIDLVVADVNVGLLYLFAVSSLGVYGVVMGGWASNNKYSLLGSIRSSAQMISYE
                     LSLGLSIVGVLMIVGSLSLTKIVEAQSSVWFILCQPLGFIIFLISAVAECSRTPFDLT
                     ECENELVAGYQTEYSSMKFGLYYLGEYAHILVVSSLAVSLFLGGWQGPFLPPVIWFLI
                     KVFAFIFFFIWIRATFPRFRYDQLMKFGWKVLFPLSLVNILVTAVFIILFFK"
     gene            28952..29362
                     /gene="nuoI"
                     /locus_tag="VEL68_15770"
     CDS             28952..29362
                     /gene="nuoI"
                     /locus_tag="VEL68_15770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HXZ37479.1"
                     /translation="MILPLLKGLKLTFRTFFTRPITIQYPEQKMVMFPRFRGLHELAR
                     DPEGMIICVACELCAAVCPANCIRVQPSEGPTHKRFPKVYEIDLGRCIFCGFCQEACP
                     YGAILLRDNYEIANYTRKDLVYNRDQLLHAYDRK"
     gene            29376..29888
                     /locus_tag="VEL68_15775"
     CDS             29376..29888
                     /locus_tag="VEL68_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXZ37480.1"
                     /translation="MLETILFVIFAGIAVVTAVLVVVQRNPVSSAIFLILTFFALAGI
                     YLLLHAEFIAAIQIIVYAGAIMVLFLFVIMLLNLEKEKRLVARNRLLKGVGVFLGVVL
                     LVQIGMVLQRVLLEGTPGKFPPEKVTALGNTQVVAQLLFTDFLLPFEITSVLLLVAII
                     GAIVLAKRQI"
     gene            29902..30204
                     /gene="nuoK"
                     /locus_tag="VEL68_15780"
     CDS             29902..30204
                     /gene="nuoK"
                     /locus_tag="VEL68_15780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXZ37481.1"
                     /translation="MVSLSSYLILSAILFSIGVVGFLLRRNTIVIFMSIELMLNAANL
                     AFVAFSRFLNSMDGQVIVVFVMSVAAAEAAVGLAIIMAMYRTQKTVNADEMNLMKW"
     gene            30328..32244
                     /gene="nuoL"
                     /locus_tag="VEL68_15785"
     CDS             30328..32244
                     /gene="nuoL"
                     /locus_tag="VEL68_15785"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXZ37482.1"
                     /translation="MLDYVGLIPLFPAIGFAINLFFGRRISKGAVGFIACAAIGLSFL
                     VSLLVFSALLKLPVAGRSVERIIYPWILSGEFQANIGFLADPLSLVMMLVVSGVSLVI
                     HIYSVGYMHDDPDYPRYFTYLNLFVFFMLILVSANNFLLMFVGWEGVGLCSYFLIGFW
                     YEKKSASDAGKKAFIVNRIGDFGFLLAIFLIFVTFGTVDFTKVFAAAPGKLEVGSTLV
                     TLITLLLFMGATGKSAQIPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMVARCNVLF
                     ILAPTSILIVAIIGAATAIFAASIGLAQNDIKRVLAYSTISQLGYMFLACGVGAFTAG
                     IFHLMTHAFFKALLFLGAGSVMHAMAGELDMRKMGALKPHMPRTYWTFLIATLAIAGI
                     FPFAGFFSKDEILWKALTQGGVVFWLIGAVAAFMTATYMFRAVFMTFHGSSRVDPHVA
                     HHLHESPGMMTVPLIILAILSVVGGFIGFPIVEGWNKFNEFLAPVFAKGVPAEGAHHA
                     VSFEVAMMLVSMAIAGLGILLAFKMYIKNPRLPDQMVERYPTPYSLIANKYWVDEIYN
                     WVFVGPLIRFSVFLWRIIDDILVDGTVNGVAAVARGGSEVFKRLQTGNIQGYGLSILV
                     GVVFLVGYLLIYTK"
     gene            32337..>32548
                     /locus_tag="VEL68_15790"
     CDS             32337..>32548
                     /locus_tag="VEL68_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HXZ37483.1"
                     /translation="MSSLAGIPLLTLITFFPMVGVLVLLFLDKEKKDTIRQVTLGVSL
                     LEFLFSLPLFFSFRLDTAAMQFVEKVP"
BASE COUNT         7123 a         8997 c         9123 g         7305 t
ORIGIN      
        1 aacaggggtc gttgttgatc accatttcat agattttgga aaggccatag gcatagctct
       61 tggaaacctc ctcgaattgc atcccgaagg accagtgggg atagcgtgag ggaaagccct
      121 ggtatgcggc gatttcgttg atctcctggt aatccaggac ctcgaagatg accgggaaga
      181 aatccaggtc ataacctcgg gcgtggtctt ctatctcata tttaaatttt ttgaggtctg
      241 agggaaattt atgtgcgggg gattgcttga gcaaggcctt tccttcttct cgaggaaacc
      301 taagaatcaa atcacgattc ttctgattcc atcctccagg cgctccactt tgaaattgat
      361 caataggccc tctctggctt gcagggcccg caggcaagcc atcatttggg cttcatggat
      421 cccgtgaagg gcttccacag acttcaactc gaccagcacc tcgtttccca ccaggaagtc
      481 gactcgatgc cacccgatat cttttccttt atagtccagc ttgatttcct tctgccggac
      541 aaaagggatc tttcgcaggg caaactcatg ggccatggct tcctcataaa cgttttccag
      601 gaggccgggc cccaattggg agtggacttc catcgcggtg gagatgatct tctcggtgag
      661 ttcctcggaa agaggacctc ccggagtctt gataaaatcc ttttgcactc tccggggttc
      721 cattcatcta ccgtcctgtt cccaggaagg atcgcaaggc gtcgaagata ctctcccggt
      781 cccgaatccg ggcgatacac attttctcgt ccccgtcgat ccgttcctgg ataaatttca
      841 taaaagaccc gctgccgtaa agggtttcca cttgcccata gcagaagaga ttggcgagtg
      901 agaggatttc cgctaagaac ttgagcgctc gttcatcgtc gtccccgaag ttgtcgccat
      961 ccgaaaaatg gaaggggtac agattgtaat cttcgggggg gaaagaccgc tggatcaact
     1021 tcaggcagag ttcgtaggcc gaagaaatct tcgtcccccc gctctcccgc gtatggaaga
     1081 aagtctccga gtccacttcc cgagcctcgg catcatgaat gatgaaacgg cgctctacgc
     1141 cccggtactg agaagtcagc caggtgtcga tccagaagga gatcagccgg acccattcct
     1201 tctgttcatc tcccatggag ccggagacat ccatcatata gatgactacc gcgttggact
     1261 cttgctccgg aagggccttc caggatcgat agagcttatc ttccttgaag ggccagactc
     1321 tcggttcctc ggggtcgtag gtcccggaaa tcacctgccg acgaagggct tggcgaaagg
     1381 tccgcttgaa gtggcgaaga gcctcaggcc cggtccgggc gatggaatgg tacttgtcct
     1441 tctcggtaac gatgttcttt ttcccccggg gctcaatccg gggcagggat aattcctccc
     1501 ccaggatgga ggccagttct tccagggtca gctctacctc taggatgtgc tcggttgggt
     1561 ccgacccagc ctctctcttg ccctggccct ccgggtcaag ggccaggggg tctcccggat
     1621 tcccctcccc ttgccccacc cctttctggg cttggccgaa ccggaaacga ggaatctcca
     1681 gctgtggcaa ggggatagaa accaggtctt tccccttgcg cccgatcatc tccccatggg
     1741 acaggtattt gcggatcccc gaacgaatcg ccccgtgaac gattttctta aatcgggccc
     1801 ggtcgcgatc tatgcgcttg gattggtaca aagtcccgcc ctgcccatgc tatttgccat
     1861 ccgacgagaa agggtccagc ctgaccctct aggggtttgt ccttggggtc ctggggctag
     1921 gctcccttcc ccaaccgacg acttatttat tttgttcctt caagtccccc cgggcgaaga
     1981 tggaggccac gaaatggagg acatccgtgg cgcagacttc gcaatacccg tagttcttga
     2041 tcagccggct tttcaccaca tcgattttct cctgggtctc cttgtccacc acgttggaga
     2101 ccaggctggt gagcttgatg gtatctttct gatcctcgaa gagtttcagc tccagagcgc
     2161 ggtgcaggcg ctcgttggaa cgataatcga aggacttgcc ttccaccgcc agggcgccga
     2221 tgtagttcat aacctcccgg cggaaatcgt cctttcggct ctcggggatc tcgatcttct
     2281 cctccaggga acgcatcagc tgctcgttgg gctcttcgct ctctccggtg tacttgtttt
     2341 tcaccttttc cttctgggtg taggccttga cgttatcgat gtagttggag caaagccgct
     2401 tgatggcctc ctcatcggcg cagatggccc tctgcacttc gttcttggcc atatcggcgt
     2461 attcgtcctt gaccacgatc atgagctccc ggaatcgttt cttctgctcc tccgaggtga
     2521 tcaggctgtg atggtccagg ccggactcca gctcattcaa gatcatgaaa gggttcacgc
     2581 atccttcggc ctcttcggag accagcgcat tggaaatttt atcctggatg taccgggggg
     2641 agatcccttc catcccttcc cgctccgcct cctctttgag ttccttgatg ttctcttcgg
     2701 tgaatccggg aagggttttg ccgttgtaca atttcagctt ttgcaggagg gtcaggttgg
     2761 ccttcttggc ctctttgagc cgggtcagga ctgcccacat ggccgccatt tcaatcgtgt
     2821 ggggggcaat gtgctttccc tgtacccggt tggggccgta gtccttttca tagaccttga
     2881 tctcatcgga aagccgggta atgtaaggaa tgtccacctt gaccgtccgg tcccgaaggg
     2941 cttccatgaa ttcattgttc tgcagtttgc ggtattccgg ttcattggta tggccgatga
     3001 tgacctcgtc gatatgggtc tgggggaact tcttgggttt gatcacatgt tcctgggaag
     3061 cgcagaggag gtcgtagagg aaagccacat ccagtttcaa gacctcgaca aattcgatga
     3121 ggccacgatt ggccacgttg aactccccgt cgaaattgaa ggcccgcggg tcagaatcgg
     3181 acccgtagac tgcgatcttc cggtagttga tgtccccggt gagctcggtg gaatcctggt
     3241 tcttttcatc tttgggttga aaggtgccca ccccgattcg gtcctgctcg gaaaggatga
     3301 ggcggaaaac acggacatga tccagcacct ttttcagatc gccggcaaac ttctgcatga
     3361 gggttcgaaa ataaaaccgg caggaggggc aaaggtcgcc atcgacgacc atcttccgct
     3421 ggccattgat cttcgcgttg aggccatcga tcaaaggacc tcgcaccgcc aggggaagga
     3481 ggtgcagcgg ctcttcgttc atggggcagg gctcccccgt ctccccttga cccggaccat
     3541 tcaatcgcca ggagaaggtg tagagggctc cctcgggttt ccgagagtaa acctccagac
     3601 cctttttcag cagacggaca atggtgctct tggccgaccc cacggggccg tggagcagaa
     3661 ggatccttcg ctcggtcccg tagtgccggg atgccgactt gaaaacgttc accaagtgca
     3721 ttagaggccg ctccaggcca aagacggcct cgaggcctcc attgaccggg tcggagaaga
     3781 accggtagcg gaagagcttg tctttgttct ccaggtattc ttcagtcccg taggaaatga
     3841 tgagatcgta gacccgctgg aaggaattgc gaaggatttc gggtcgatct cccaggatct
     3901 tgaggtaatc ttcgaacgtc ccttcccaat gaagggcctg gtaggaactg tcctgggtat
     3961 agcgatcgat gagggatcgg atttcttgct gaggaagcat ggattcacct ccggttctga
     4021 ggccccgacc gactgcccgg gcatgggggc atttcctaaa ggaggcgaac tcgggaagga
     4081 acatccaccg ggtctaggct tctactattc ccggtggata gaagaactgg aattggagaa
     4141 tttcaggcaa tgccgaacgc tacccggagc attttgccag ggccgttcgg ggttatcgga
     4201 gcaaatcgca tgccatcttc catccaccaa ataacctaaa gattttattc gtgaaaggtg
     4261 agtttacccg gatgtgactc aaaaaggatc cttcatcttt ccacctgaag agtcaaattt
     4321 ttgccgcccg gttttatggc attatgcgat gggaaacgga actcgacagc taagctcagg
     4381 cctcgttttg tagcctatct cttctgggcc cctgaaatga acttttggga gagggaaaag
     4441 gtttgcaaac ctattccatc tggagtaaaa ttttttagcg tatttctgta cctcccagaa
     4501 cgggttgccg tcgcctccag aaaaaatata tcaacctagg ttcgctatgg ggaacacgct
     4561 cctgtgcgaa atcgtggggc gcccaatcac tatttcttta tgttctctga attctcaaga
     4621 gtaaataaac ataaaggagg agggccatga attctcgcga aaaagtgttt tttgaatcct
     4681 tccgggaagt ggtaaaggcc atccactcca ctctggacat tcggcaagtc ctgcaaatgt
     4741 tggccactaa ggtgagccaa gtgatgaacc tgaagggatg cgccattcgc ttgctcgacc
     4801 ccacgaagcg gacgctcgaa cttgtcgccg cccatggatt gagtgagaag tatatttcta
     4861 agggagccgt ggccgcagac cgcagcatag cggaagccat ggaaggaaaa acggtgagca
     4921 tctacaatgc caccaaggac ccccgcgccc agtataagaa agaggcggag gaggagggaa
     4981 tctacagtat tgtttccgtt cccttttcga tcaaaggacg gatcatcggt gtcctgcggc
     5041 tctatacttc ccaaccaagg aatttttccc aagaagaaat aggctttttt gaagccctgg
     5101 cggaaatggg ggccttggcc attgaaaacg ccaggatgta tgaacagctg cggaaggatt
     5161 atgaaaacct gatgagtgac atcttgatgt tcgtggggta ccggaggagt atctaagggt
     5221 attgccgcct aggcgcggag tgctcccatc ttcgcctcga attttcctgt ttttacgaac
     5281 ctctctttgc ttcctggatt ttttccagcg tatcccggta gatatctcct tttcctggat
     5341 ttaaccgcag cgctttttgg gctagagtct cggcctcatc caatttttcc ctccgagtgt
     5401 aatatagcca tgctaggttg ttgtaggcgt cggcgttgtc gggttctttc tcgatggcca
     5461 gtcgataata atcttcggcc tttttccatt ccttcttctg gaaatgggca tttcccaggt
     5521 aaagaagagc tccgggcagg ttcttggccg ccaggttgta ttccttgatg gcgttgtcga
     5581 aatccccctg ctgttcatag gtcaccccga ggttcaggtg ctcctccggg gtcagggggt
     5641 ccttcaggat gatgactcgg gggaaggaac aacccgtcgc aaaaagcaaa gagcaaaggg
     5701 ccaagagcaa aagaaaattc cttttcgtca tgggggggtg atccgcaggg tccagaattt
     5761 ggttttctcc caggtcttta aaaatgaatc cagggatatg aacttgtttt cttccttgcc
     5821 agaattgaca acgatgcccc ctttgtcata acccacgaca accatgaaat ggtggttctg
     5881 gtagacccag aatccctgat cgatcaagac aatgagaggt cgccgggaat caatgctgcc
     5941 ctgtaaatcc tccaaactgc ctgcgtactg ctctgctttc attcctttcc tctgggcgta
     6001 gaaaaccata tccatgtcca gcgtcccccg ggcactccgg ctgaagacct cggccgcaat
     6061 ctctgcgggg gtaatccgga ggccgtaata attcaaaacc ccggccagtg atgcagggcc
     6121 gcactgaaac tgctcctggg ggaaaaaggg gactccttca atcgctcggg ctgtctttag
     6181 ggatagcatt ggagaggggc cggcgcagct ccaatggaag gaaatcgcga aaagaatgac
     6241 caggcacacc cccgtcaggg ggagtctgcc ttctccggtc ggggatgaaa atcgaccttt
     6301 gtttggcttg aacaaaaggg gttgaatctc attccttttt gatgacaatc ttgtggccca
     6361 gcagctgaat gatgacgatc accaggatgg cgatgatcag cagggcgatg atgatcccca
     6421 aggcgctgtc tccggccact ttcaggtcgt caagcttcgt ggccaattga tgaatctgtt
     6481 cgtcgttcaa ctggctaagg cgcgattgta tctcctcgga agtgtagccc aactctttca
     6541 gccgttctcc caccatcttc atttccagaa attgccggac tttctggagg tcggcggccc
     6601 gctcgcccgg ggaaagcccc acgacctccg acggagcaaa cccggcgtat acgcggggag
     6661 taatcccgat gacgaacatg gccacaacca ggtaccaggc aatccatctg ttgagaaatc
     6721 gggccattgg tgtccctcct tatttctttt accgaattcc ggaatccatc gtttccctcc
     6781 aacctcagaa aatagtatga aaagaaacat cgggtgtcaa gcagaagatc gatcccggcc
     6841 ggctgacctc cgcgagttct tttgttggcc cggggactca ggctaaacac cgagaaaaga
     6901 acccccggaa agaaatcaga atatattttt tattcacccg gccgcagaaa gaatttgaca
     6961 aaataatttt tttgttgtta tctaggaagg aaagggatga aattaatttt ctcgagaatt
     7021 tatgagcatt cattccaacg gttgacccgg gggttccctt ccggggggta ggcaagcggg
     7081 ttgttctatc ccataattcc tctgctctgg gccaaaagga aaagggtatg acctcaaaag
     7141 aattcctcac caccggagaa gccgcgaagc tggtcaatat ctcccggtca accatttccc
     7201 gcaatttcga caagggcatc tttcagggaa agaagaaccc cattacgggg gaacgcctca
     7261 tcagcaggga gagcctgatc aacttcatga ggcaatacga ccttcccgtg gataccctgg
     7321 agctgggaaa gaaaaggatc ctcgtgggga gctcggacga tcaactattt tccctgctgc
     7381 aaagaactct ctccgaagac gaccggcttg agctggagag ggttgtcttc ggaggagatg
     7441 tgttgatttg gtgctcaaag gaaaacccgg accttctggt catcgacgaa gagctcccag
     7501 acatctccag tatggagatt atccggtcgc tacgaaggac ggacaatcag caggccctga
     7561 agatcatctg cgcagggaag gcccgcagcg tgagacactg cctggattgg ggagcggatg
     7621 aagtcctggg gaaggaaagc ttgagccgcg aggaactggc caagacgatc tatgccttcc
     7681 tcaatcttcc tcaggaaatt cccgaagagg aacggcaata cagccatcaa aggcggtggc
     7741 ctcggatggc cctccatctt ccattgaaga tcggggtcta ttccgtcagg actccctatc
     7801 gccgggacac gggcctggcc acgatggaaa acatcagcct tggaggggcc ttcctttccg
     7861 aattttcgct ggaaggcggc atgattcctt gtgaaccctt ccggattctc ctggaagccg
     7921 atcaggagcc tctaaaggat tggaaggctc acggcaaagt ggtacgcctc cagtccaacg
     7981 gtacggtatc cacaggcgtc caattcactc gactgcccaa agccagcctg aagatgatcg
     8041 aagccttagc tcagtaaccc aatcttccgg gtttttcacc tgccgtcccc tcgcagagat
     8101 tttttttaaa caaggttgcc ggatagtcgc cggttgctga aagattttgc agttttatag
     8161 gaatgaattc aagctggacc caattcgaac tcgagccccg aaacgaaagg agacaggcaa
     8221 tatgtcatct tccataacaa aagacgaatc tcaaatgagc cgcagggagt tcttgaaagc
     8281 cgcttccgtg ggagccgccg gattggccct catgggattg ccttccggca ggagcgaggc
     8341 agtggagaaa tatcctcatt ggggggaggc cgtggtcatc cgggacgaga gcgccacgtt
     8401 cggcccgaat attgatagta aagtggtgcg agaaatgctt aatgaagccg tcaaggccct
     8461 tacaaacggc ggagggtggc cctctctctt ttcttcctat aagaccggag aaacagtggg
     8521 catcaaggtg aactgccagt ggccgcgggt gccgacccag cccaaggtgg tggcggccat
     8581 cgtcaatgaa ctggtgaaaa tggaaatccc gaaagataaa atcatcattt gggacgccga
     8641 ggcgggcgcc ctaggatatt cggaaatgtg gaacgtcagc atggagttcc ggggagtgcg
     8701 caccgccgcc acggataaac tggaggaccc gtttgatgca agtaagacca tggatatgca
     8761 ggggaatccg gcccaactga gccgattgct gacgggcccc gcccacctga tcaacgttcc
     8821 ggtgctcaag aaccactttg aggccgggat tacctttgcc ctcaaaaatc atgtgggctc
     8881 cgtggggaag gacaataaat cccggcagat tctgtttcat catcccatgg atgaaaaaaa
     8941 tcctatccat aaaatggcgg gacggggcgc tcccatttcc gagcgcatcg cgctgatcaa
     9001 tacggctccg gatatcaaaa ataagacacg cttgatcgtg ggagacgctc tcttcggtgt
     9061 atttacgcgc gggcccttgg gagagccgga atttgcctac aacggcctga tcgttggaac
     9121 ggacccggtg gccaccgatc atcaagcccg gctgatcatc gagggggaaa ggaaaaaaag
     9181 taaccttccc ttcgttcctt ctttgcacat tgaccacgcc ataaagttgg acctcggggc
     9241 tccgctcgag gaaatgaagg ttacccccat ccatcgaacc aaaaagggat agaattgacg
     9301 ggatgaagcc aggggttcat ccgctgatga ttttgatatt cttgatcctg tccgcagggt
     9361 cggcctgcgc ggatcaggct tcggcgagcc ccgagaagct ccttgcagca atggatctgc
     9421 ttcccttgga tgatgacctt ccggggtgga agcgctcgga gaagcttctc agcgcctcaa
     9481 atgaggagga gctttataag atcctgaacg ggggagcaac cctttacatt caacatggtt
     9541 tccaggtgtt tgtgggacag agttatagag gaccgaaagg ggtggaatcg gaagtataca
     9601 tttttgatca aggcaccgct caaaatgcac ggggtctcta cgaaaatcca ttggccaaac
     9661 cgggccgcag caaagaggtt cccgatctgg gagaagaagc gcggatcgat accacacctc
     9721 ttttctctta tggggtggat ttcgttcaaa agggtttctt cgttcgggtc attatccagg
     9781 aaaagacgga agaagggttg aaggtggccc taacgtttgc tcacaatata tccaaacgaa
     9841 ttaagaatag aatgtctcgc cgataaatcg aaatgcccgc aaaagagggg ccgcaaaatg
     9901 cttaccgggc agggcacggc gtccaggatt tcgaccttta tttaatttct gaaaattgaa
     9961 gaggcaggac tcgggggaaa cccagggtct cattttgacc cggattttga agaggatccg
    10021 ggaagggaac cgcccaccta ataaacggcg tccggaaaag tagtggtggt gcggggttgg
    10081 ccgaccagga ggtacatctt gggcgagtac cagccttcat cgaggacatc atcgtcgatt
    10141 tcgctgacca ccccatcgtg gatggcccag gagcggccgc ggacggtttt ctcgaacccc
    10201 agctggcgga agggggtcga accatctagc cgcctgaaaa gctttcctcc gggctgaaag
    10261 gcttggtgaa tccgggtcat ccgtttgaaa ccctcgaaat aggtaaaccc gcaacgctca
    10321 tatacgatcg cgttgtggta aaagagcgct tccaggctga tcgttttaat atccagaatc
    10381 ctcgcaaaat attccagccc ggtgatgact tcccgggtca tccccaggcc cttacggacc
    10441 tgaccggggg acatgcccga ccgcagggcc ttgatttctt ctggcaggtt gcgggaaccc
    10501 cttccccaca gggtatcccg gccttcttca tcgacatcga tgttgaaccg atcgctgtcg
    10561 ggatcgttca cgatcagaaa gtcccagttc acctgggtaa agtccggccc gtctgacgct
    10621 tggatggagt aaatggggtc cgggtcgtcg agtttgcgct tcacctcgat catcaccgtt
    10681 tcttcccgct cggggcagta gaaccgaacc aaccgattgc cttcgtagtc cgtaaaggtc
    10741 agcgggttga tctggaaccg cttaaagagc gatggaggga tgagcaggcg gtacaggcct
    10801 tctttttcgc tttgggacat ggagtttatg cgtttgagcc agggcataag agaagcagtg
    10861 tcagtgtcag tgataagtgt caggaggccc ggttttgctg acactgacac gaaccactga
    10921 cactgtattc ttagaggagt tcttccaccc acttgctccc gccattgatc tcatcaatca
    10981 atttaaggaa gcgatgggcc ttaaattcat ccttggaggt caaggcgggt ttggcctttt
    11041 ccagacgatc catcacttga aaagtgtccg cccgggtcag aatcaggggg attccccgat
    11101 cctgcgccac cctgactact ccttcacttg gattttttcc tccggtcaaa atgattccag
    11161 tcacgggttt ttcatcgggg ttcaaaccca ccagaatgac cttgttatat acctgtttga
    11221 agagggagaa aggcccttcc aggtatttgg cccctatggt cgacgtctgc actaaattgt
    11281 tttctccctg aagacagcag aggatttccc ccccggtgat atcggctatg ctggaaaccg
    11341 tgagggccgc cagaatcggg tcttcaggaa cggccatcgc cgactttact cctttctgct
    11401 ggaggaagga aaccaccttg gtcttcacgt ggtccacctt atccggagcc acatggttaa
    11461 gaatggccga tttcacgcgt ccatcaagga aggagttcag agaaagaacc gagtaaaaag
    11521 tcaggttatc ccgctggtag cggtctacaa gaagaacgaa ggaatcaaaa gatttgacta
    11581 atgcaccatc cgaaaggccg gggcctcctt ctccgaaaaa aatctccttg ccccccataa
    11641 ttaaaattac atccttatcc cgggaaactt cctggaaggc cttctgtatt tttcccatga
    11701 gctcttcgcc ctgaatcccc gcgaaagctt gattccccgg aagagaaatc gggcagaggg
    11761 tttcatccgg ctcggatact cccaagactt gcttgaacaa aagaacgtcg gggtcgcaag
    11821 aactgccggg aggagagatg ggggattccg ggaggagacc gtaaggcttg aagaaaccga
    11881 ccttcaggcc tttggccttc agtcgaacgg ccaaggccca tgtggccagg gtctggcccg
    11941 gtccactccc cgtggacccg atgaagatgg aacgcatgga agacctccgt ttgttcaccg
    12001 caggggatgc gaagaacgca gaggagataa agctagcaat ttcatggggc ggaggatgaa
    12061 ccgccctgcg ctggcaccgg aaatattcta gatggatgat accagaatgg aggggggatg
    12121 taaatcggta ggggcggttc gcgacccgcc cctacttctt ggtatagacc aggttgattg
    12181 gatggcattt gtcgcaggcc tgggttcggg gatgggcctg gtggcactgg tagcaggtct
    12241 ccatggtagg gcggttggtt cggggagtcc ctttgtcatg ggccaaattt cggtggcagt
    12301 cgatgcacaa caccttcttg tcggcgtgct gcttgtggga tactttgacc acccggacct
    12361 tgtccaggtc catttgttcc ccccccggga tgtttggatg gcagcccgcg catctttggc
    12421 tcatcaggtc atcgctggcc gaaaaactgt gggaaaaggg agtgcctcct ctcgagatgt
    12481 ggcagtccac acagtccacc gaagccggat gcatccggga gggaagccat ctttcgggaa
    12541 tatcctggtt cttgtgacag gtcaagcaga agaaggggtg gctggtggta aaccggacga
    12601 tgccgagggc tggaatcgct gccaggatta gaaggatggc aacgataaag gcccatgtgc
    12661 gcttggacag cttgggtatg gctggtattt tcatggcctg ccctcgaagt cgaattgaag
    12721 gaattctttt tgcgcctccc ctcgcgggag aaaatggaat ggggagagaa ttcaataccc
    12781 tcgatcagat tttggttatg gtgaccagcg tatcgttcca ggccgttcct ccggcagccg
    12841 ggtcgggctg ggccggaatc accgagttag ggttggcccc atttccttct tgctcccacc
    12901 agagctcgtt ggtgtcaaaa tcgctactct tgtattttat ggccctggca atctttccca
    12961 gtgcggtgtg ccccagccct tcgggcagag ccaccacttt ggggtgtatg ccgtgagtca
    13021 tccggatggg aaccgtcaag gacccagccg aggaagtgat cttgacccgg tccccgttct
    13081 tcagccccat ggcctgcgcg gtccggaggt tcacccacaa ggggctggcg tggagaatct
    13141 ccgccagcca tttggagttg gccaggcgaa gggtcatgac gttgggccgg tatacggtca
    13201 ggaagaaatc cgtctctttc gtttcttgat gatttttcac cgggacatag gtcggcaggg
    13261 gaggaaaccc tttctcctgg agcctggcgg aataaatctc aaccttgccg gagggagtgg
    13321 gaaacccttt cttttcgtaa gaacggtact ccggcttggc cgccggatcg agccaaactc
    13381 cctctttctt gagaaccgcc agccctcccg ctttggcaag cccctcgatc ctcgcggcag
    13441 ctttttccat gaactcctcg ctatggccgt agggaaaagc cttgaccata tcgtccccca
    13501 accgcttggc caagctggac agggcatccc ccagggatag ggactttccc agggggagta
    13561 ctaccggttg acggaggctg acgaaaggca caagccccat ggccggccgg gattccagat
    13621 tccagctctc caggtaggtg gccatgggaa gaaggaggtc ggccaaggcc ccggtttcgg
    13681 tcaaatgggt gtcggccaca accaggaaag ggatcctctt ctcgtctttc aaaagctttt
    13741 ccacctcagc tgagggaggg gtggcgtatg ctgggttggc ctggtagcag aaataaaagt
    13801 ctggttgacc cttgccctcc tttagggcgg tgagggcctg gaaggaattg ttgaagggac
    13861 ccttcccttt gggctctccc aggtccatct tacggggaag acagcaaccc ccgggaacat
    13921 ccgcgctgcc caccactgcg ctgagaaggg cgatgcagcg ggtgttcatg accccgttct
    13981 gatggccgct caccccccgc ccgaagagga tggcggccgg tttggcctgg gccaattctt
    14041 tggctaagcg catgatgtcc acggccttga ccccgctcac cttctccgct tcttccggcg
    14101 tgtaggagga gagttgctcc accagcttgg acaggggtac attggtccat cgggaaagga
    14161 actctttgtc gtgaagcccc tgctggatga tgtgctgggc catggccaga gccacgattc
    14221 cgtcggtccc cggcttgatg ggaaaccatt cattgctctt tccagcggta ttggagaggc
    14281 ggacgtcgaa ggtcaccagc ttggccgcgt tggtcatccg tccttcgatg atccgttgaa
    14341 tcaaggggat gtaaagatca tgattctcgt aggggttaga cccgaaattc aggatgaacc
    14401 tggaacgggc ggcgtcgcag accatcgtat ccgctcccca ggtcatcatc tggccgactt
    14461 tttcgttggg attcatccac tccgaatccg agaagacgat gggacggccg aaggccttca
    14521 agaactccag ggccatgagg tcctgggagc ccggtgtgtt catctccatc caaagagctt
    14581 ctgtccggcc gctcttcttt agggccccca tccgcttggc catctcgtcc aaggcctgat
    14641 cccaggtaat ccgggtccat ttcccctccc cccttcctcc agaccgcttc atgggctgga
    14701 ggaggcggtc cgggtcgtaa agggtgttga ttcccgcatg cccgcgggag cagagcttcc
    14761 cccggttgtt gggatgcttg gggttcccta ccatcttttc cagccgtccg tccagaacct
    14821 cgccgacgat tccacaccct gccgggcaca ggaggcaggt gctcgcaacg gaagcggctt
    14881 ttcccgaggt gcgggtcacc tttctcggaa cttccctcgg agggctgaag cagccggcca
    14941 aggacccgcc gaggccggct acggtcgcgc tggtaaatcc caccttcaaa aaatcgcgtc
    15001 gggtaaattt catgaaaggc tccttaaacc tcagaccagg ggcaagaact caccgcccag
    15061 gaccacgaca taccgcatga aaaagatccc caccacgacc agaatggagg cgatggtaat
    15121 ggtggccagg tttcggaacc ggggaaccaa aaggatgatg gccggaatga tctttcccat
    15181 cacgttctct acccagatga agtagatgct gaatttcccc gtcaggatga ggtgggccac
    15241 ctcctgggct tccacatggg agaccaaaag gaccaggacg tcgctcagga ccaggaaaag
    15301 gtctataagg atcatggctg ccagcatctt acccagaccg aagaccagct cccggttgat
    15361 ggttttctcg cgggagaaga acttgtcttt aaccatagag ataagaatca tcagggcgat
    15421 tcccgagacc atggccgaga tcaggaagag gaagggcatc aaggccgtgt tccagagggc
    15481 ccgcgctttc cccagggcca ggatgaaacc cgtatacccg tggacgaaga tggccagggg
    15541 gatcccgata gtcccgaaga ggcgggtcag aggcttattt cccttgaaca tgaagtagcc
    15601 gtagatgatg caattgatcg ggtacagagt cagcaggaaa gacccccaag taatcggcga
    15661 agtaatgttc aggtggacaa agagatgcca ggacttgaac ggctgaccca cgtgaaccag
    15721 cagggccagc ggggccatga ccaggaacag ggtcgctaga atcactccga tcttcccgat
    15781 gggcttgaac ttctccatcc cgaagacaaa ggccatagtg gagagaacga aagacccggc
    15841 gctcaggccg gtgaggtaga aataaacggc gataagcgcc ccaaaggttt cctggggaaa
    15901 aacattgtaa aggataactg catccatctc attccttcct tatcggtcct caggattaat
    15961 tcctccgccc cgggaatagg ggtcccaaga aaggtgatct acccaggctt tgtccctgga
    16021 ccgaactggc ggcgatcctc ctccccctca agccccgggg gttctgaagt cttttcgcaa
    16081 tttggcccga atcgaacgag ccttcctcct agaaagtgtt tttatcgagc cccttggaaa
    16141 ccccaggccc tccgctgggg aggagccgag tcctgcttac agcttggcat acagcagaat
    16201 ggaaatgacc agagcgtaaa tgaccaagga ttcgatcagg gccaatccta cgatcatggg
    16261 gatgaaaatc ttgggctggg cttccgggtt cctggcaatg ccctccaggc ctttggagat
    16321 ggcgatcccc tgtcccaagc cccctcccaa ggccgcgatc gcaatggcca gaccgcaggc
    16381 caggccgatg aacttcttgg cttcctggcc caaggcttct tcagcggcaa aggccaagga
    16441 tgcaaaggct aaaatcaaaa ccaaagtcaa ggccaaaatc cccaatcctt ttcttctcat
    16501 tctcccttac ctcccgttgg tttgaaatca aatcacccta agacctttct caatatgcga
    16561 aggtcctcca ccttccgtct tcccattccg ggggagcggg aaagagagga ttccagatgt
    16621 cgaaatagct ttgccgatga cctaatgttc agactcctcc accgccaggg aaatgtagat
    16681 catagaaagg agcatgaata taaaggtctg gatgagggaa acgaacaccc ccaggaaaag
    16741 ggacatgggg gaaagaaaca ggggcaggat caaaggagcc gcaagggtca gagagaggag
    16801 catatgatcg gccatgatat tcccgaaaag acgcatggat agggaaagga cccgggccag
    16861 gtggctgatg atctcgatgg ggagcatcaa gggggatagc cagaggaccg ggccgagaaa
    16921 atgcctgagg tacttgatcc cgtgggtctt cagcccatag taatgggtgg cgaaaaagac
    16981 caccagggcg caggccagag tggtgttcag gttgtccgtg gggggcagaa atcctggaac
    17041 cagccccagg aagttggaaa aaaagataaa gaaccccagc gtcccaatga gggggaggaa
    17101 ttcccgcccc cggtgcccga taatatcatc caggaatttc agcaccatct cggtgaagag
    17161 ttcgaagata ttgcgaacgg tcagccgatc ggaagggacc agggcttctc ctgccggccc
    17221 tgaggcgatc ttcttaaacc ctgcataagc cagaatgcac aaaagcccca ggacaaatag
    17281 agccatggaa acctggtgcg gcaggcttcc gatccccgga atgtaatgga accaggtaaa
    17341 gggggcgtgt ccggccatta acctactccc tttctattgc tccgtagaga ttgtcggacg
    17401 acctcaaaaa ccatccccaa aaccaacgtg gagaccccaa cgaccaaagc caccgggttc
    17461 acccggacga agcggatgac caagaagagg accagcagca agaaaaagaa cttcccgatc
    17521 agctggaaga gggaatattt cttcttcgcc atcaggtatt gactcaaaat tcgccagagc
    17581 caacgaaaat tgagcagggc gatccctcca cccaggatca atcctagtga gacggcccag
    17641 ctttgaaacc agagactcgc caggagcaag acagccaaaa tctgggcgct gcgtttctcg
    17701 atggagaata gcttctgggc ttcttgatgg ttcgacaaca gagggagatt acttttttcg
    17761 ttctcttttg tcaatctctt tcgccaggga gaagagcctg cggaaccctg cggcaacacc
    17821 cagaagcaag aaaatgattg tgagccacgg tttggtttgg aaatatcgat ccagataata
    17881 gccgattgcc aatccgatgg ccacggagag acccatctcc aagccaatgg cgctgaaacg
    17941 cgcaaactgc cggtagaatc tctttttatc ttcctccatc cgatctaggt tcccagccct
    18001 gtttttttct catcatagca ggttcctaga ctcaatccaa tctcctcgct tagcccctct
    18061 ttttagccta atgaatgaat attcattcaa aagtttagta acatatcccc tcaaaaaatg
    18121 tcaaggagtt ttttcgaaaa tttacggata ttcgagctaa tattttttcg ccacctaaga
    18181 ttttgaaatt atatcctatt tcactttttt cgagccaata ttcttgattt gcatcagcgg
    18241 ggttttggcc gaatcaaagg ggggagaaca tttgttggtg gtctttggaa tcgctagtta
    18301 tgaactcgtc aaaagtaaaa attccataaa aattgtcatc ccagcaaaac ccagaattca
    18361 gttatttcaa gaagttctgg accctgacgc ctgccccgga catgatcagg ggttcgcagg
    18421 gctaatgatc ctgaaggctt tttgcgaaac catcaccagc gaaaggtttc agaaataagt
    18481 catactggac tttttgtgtt ctctcttgga gaagaggagc cgataatcct tgatcccggt
    18541 tttcttcgag attgcttggg ctatccggcg gcaatccttt ccgccttttc cgtgaaccat
    18601 tgtatagagg ttaaaaggcc aatcgggaaa gggcggacgg aggtaacaat ggctgacagg
    18661 gggaaaggac acaatggtct ggctgactct ggggacctgt tcttcgggca ccttccagac
    18721 taccatggcg tttccctcgt atccggcttt ctgatgacgt aaaatcgccc cgaacctccg
    18781 gatgattccc ccgcggacga accctcggac ccggcggagt aggacctttt ctttccaccc
    18841 tgcattccgg gcaacctgga cgaagggagt cagggatagc ggcaggtccc cctggatttg
    18901 ccccagaagg cgtttatcct tcggagagag tttttcattc ttcatagact ttcatcgtcc
    18961 catgcacaca ctggaactat gatctatact atagacgaaa aatgagaatc aagctattat
    19021 ttgatccgga gagttgagga aagatcgggc ggtgctgctt taccaataag ataataacga
    19081 ttcgggacag cacccggtcg agcagcagca taccaagaat tttgagaatt tagccgtctc
    19141 cggtgaccga tcgttcttgc caatttctgg gccgaagaaa aaaagacttg acataaagcc
    19201 tgctcttttg ttatttcctt gggccgaatt cgtctccagc caattttagg gttgcctttt
    19261 taaaaaaaat attataaaga attttggata aggaacgttt ggggattgtg gttaactctc
    19321 cgcgctcctc ttttgctttc ttaatcttac ccggtaaaac tcctttgatg aaggggaatg
    19381 aatccatgct gactgactac cttcccatct ttatcttctt tctggtggcc ctggggtttg
    19441 ccctgttcac catcgctgcc tcggcttttc tgggcaggcg aaaaatcacc ccgcaaaaga
    19501 tgatgcccta tgaatgcggg atggaccccg tcggtcaggc ccgtaatcct ttctcggtga
    19561 agttttacat catcgccatg ctcttcatca ttttcgacat cgaagccgtt ttcatctatc
    19621 cctgggcagt gattttccgg gacctgaaaa tcttcggact ggtggagatg gccgtcttcg
    19681 tcgccatcct gctcgtcggc ttcatctatg tttggaaaaa aggagccctg gaatgggaat
    19741 agaaacacaa ctgggcaaca atgtcttaac cgcttccttg gagaagctgg tcaactgggc
    19801 ccggcgttcc tccctctggc cggccgggtt cggactggcc tgctgcgccc tggagatgat
    19861 tgtcgccgca gcggcgcgtt atgacatggc ccgcttcggc atggaagtct tccgcccctc
    19921 accccgccag gcggacctca tgattgtggc tgggacggta accaaaaaga tgcttccggc
    19981 ggtcatccgg atctacgagc agatgcccga gcccaaatgg gtcatcgcca tgggagcctg
    20041 cgcttcgacg gggggaattt tcgacaccta ctcaacagtt caggggatcg accagtacct
    20101 accggtggac gtctacattc ctggctgtcc cccccggccg gaagctctcc tttatgggat
    20161 catcaagctc cacgagaaga tcggcagaga gaagctgctg gaaaggtaag gaaccatgga
    20221 cctgaccgct gttgtggacc ggataaaaaa ggaaatcccc caatcgatcc tcggggtctc
    20281 tgccttccgg gaagagacca ccctggagat tcgtgccgag gaccttctgt ccttgtgccg
    20341 ctttctccat gacgacccgg aaatggcttt tgactttctg atcgatctct gcggcgtcga
    20401 taacctgccc cgtgaacccc ggttccaggt ggtttatcac ctttgttcca ccaaaacccg
    20461 cttgcggctc cgtctgaagg tctctctgcc gggaaagaat ccaaagatcg cctccgtttt
    20521 tccggtttgg aaagcagcgg actggttgga gcgggaggcc tttgatatgt acggaatcac
    20581 ctttactggc catccggacc ttcggcgcat tctgctgacc ccggattggc aggggcatcc
    20641 cttgcgcaaa gattacccgc tccggggcta accaacctcc cggacgatgg agaaggatgg
    20701 agatgggcga caacagtacc atgatcctta atatggggcc ccagcatccc agcacccacg
    20761 gcgttctgcg ggtgattctg gagctggacg gcgaggtggt ggtcaaagcc acaccggata
    20821 ttggtttcct ccaccgcggc ctggagaagc tggccgaaag caagacctac caccaatgca
    20881 ttcctctcac cgaccgcctg gattacgtgg ccggtatgtc caacaacctg gcctatgttc
    20941 tggccgtgga gaagcttctg gggcttgaag ttcccccccg agcgcaggcc ctccgggtcc
    21001 tcctcagcga gttgcagagg atatccagcc acctcctttg gctggggacc catgcccacg
    21061 acatcggggc catgactccc ttgttctatg ccatgcggga gcgcgatgag atcctcttcc
    21121 tcttcgagag agtctgcggt tcccggctga ccccttctta cttacgggtc ggcggcttgg
    21181 cccaggacct gcccgagggc tttgaagaga aatgccggga attcacccag accttcaacg
    21241 cccacgtaga tgagtacgag acgcttttga cccggaatca gatctggatg aaccggaccc
    21301 agggggtggg agtcatttcg acggaagagg ccattaactg ggggctgacc ggcccgaacc
    21361 tccgggcctc caacggaaac tgggacctgc gcaaggcttt cccctacagc gggtatgaga
    21421 aatatgaatt cgtcgtcccc acggggaaaa acggggatgt ctacgaccgg tatatggtgc
    21481 ggatgatgga aatgcgccag tccaatgaga tcgtccgcca ggttctggac aagatgcccg
    21541 gtggtccggt gatcgcccaa gacccaaagg tgatctatcc gcccaaggac cgtgtgcagg
    21601 aaagcatcga atccctgatc catcattttc acctggccag tgccggtttc cccgctcccg
    21661 ccggcgaagt gtacgccagc atcgagtccc ccaaagggga gctgggattc tacctggtca
    21721 gcgacgggac gaacaggccc tggcggttcc gcgtccggcc gccctcgttc gtcaacctaa
    21781 gcgccctggc ggtgatggtc cagggacggc tcatatcgga cgtggtggcg gtcatcggat
    21841 cgatcgacat cgtcctggcc gaaatcgacc gctgagcggg aggagggccg gccccatgac
    21901 cctgcgggaa gctgccgaga aactgggatt aaagcccgtc gcgggcctgt ccaggctctc
    21961 ccgggagttc ggtcaggggt atgtgagcga ccttctgagc gacgtcatgg ccaatgccaa
    22021 gccgggggat atctgggtga ccctccagat ccaccagaac atcatcgccg tggcgacact
    22081 caaggaactg gcgggaatca ttctcatcag cggcaggcag ccggaaaaac agacgttggc
    22141 caaggcggag gaggagcaag ttcctctcct cctttccgac ctgccggctt ttgaggtggt
    22201 gggacaactg tatcagatgg gaatcaccgg acgaagatga tcccgtttcg accgtgcagg
    22261 atctatcttt acacatattg gatatcgtgg aaaactccct ggccgccgga gcgcggcggg
    22321 tggagattcg agtcgaagag gatatggctg cggatcggat ggtcatcgag attgtcgatg
    22381 acggctgcgg gatggacgag gagagggccc gcaaagccct ggatcccttc ttcaccacca
    22441 agacggtgcg acgtgtgggt ctgggcctgc cgctcctgtc cgaggcctgt cggatgagca
    22501 atggggaatt ccggcttgag tcctgcccgg gaaaaggaac gcgggtgaga gctgcctttc
    22561 aacacagcca catcgaccgg aaacccctgg ggaatatgag ggacaccctg atcaccctga
    22621 tgagcgggca cccgcaactg gacctcctct atgagcaccg gaaggagggg aaaacattct
    22681 ccctggatac tcgggagatc aaagcggacc tgggggagat tcccctggat gcccctgcag
    22741 tcgtatcagc cctgcggacg ctcatccagt ccgggctgga agacttggga gctttttagc
    22801 acaggatgca tttggcaccc gggtcgggga gaaagattgg aacgttttta gggaaggggg
    22861 aaaggaatgg ccaagctatc catcgaagac ctgaaaaaga taaaggagtc ggtgcgggcc
    22921 gcggtgaccc ttcgggaagg gggctaccgg gccagggtca acgtccacat gggcacctgc
    22981 gggctggcgg ccggggcccg ggcgatcatg accgcggttc tggatgagat ccagaaaagg
    23041 gacaccaaag acgtcgtcgt ggtgacctcg ggatgcgccg ggctctgcaa tcgggagccc
    23101 atgatcaccg tggaacggat gggggagccc ccggtgaagt acgtggattt gactccggat
    23161 aaaatgcggc agattttttc cgagcatgtc ctggaaggga agatcgttga aaaatacgct
    23221 ctggcaatcg ggagtgaaac gacgttttaa acaaagtgac ctaaaatgcc taaagtgcct
    23281 ggagtgccta gagtcgaagg cattcgaaca tagaacacct ttcatccttt aactttaggc
    23341 actctaggca ctccgaactc caggcacttt tattgggtct tagggaggtt gaggatggca
    23401 gccaaggtat ttcgttccca tatattgatg tgcgccggaa cggcctgcgt ttcctgcgga
    23461 agctttaagg tccgggagcg gctggagaag gagctcaagg ctcgcgggct ggatgaggaa
    23521 gtgaaggtgg tcatcacagg atgcaacgga ttctgcgccg aaggccccat catggtggtg
    23581 taccccgaag gaatcttcta cggccatgtg acccctgaag acatgcccct ggtcgtggaa
    23641 gagcatatta tgaaaggccg cccggttccc aagctgttct acaaggaacc ggtcaagaag
    23701 gaagccattc cccggatgag ggacattccc ttcttcggcc tccaggtgct gcgggtcctg
    23761 cgcaaccgcg ggctgatcga ccccgaagcc atcgacgaat acatcgccaa ggacggatac
    23821 gtggcgctgc accaggcttt aacccagatg actcccgggc agatcatcgc cgaggtcaag
    23881 gaatccggac tgcggggccg ggggggggcg gggtttccca cgggagataa gtgggaggaa
    23941 tgccggaaat atgaccgttt cccgaaatac gtgatctgca acggcgacga aggggacccc
    24001 ggggccttca tggaccggtc ggtcctggag gccgaccccc acgccgtcct ggaaggcatg
    24061 gccatctgcg ccaaagccgt ggattctcac gaagggtatc tctacgtccg cgccgagtat
    24121 cccctggcca tcaaacgggt aaaccgggct atcgagcagg cccggaatta cggcctcatg
    24181 ggaaaggaca tcttcggaac cggcttcgat tttgacgcct tcgtctatcc cggggccggg
    24241 gcttttgtct gcggcgagtc cacggccctc atgttttccc tcgaagggaa gaggggaatg
    24301 ccccgggcca aacccccccg ctcggccgaa gccgggctct ggggtcagcc cacggtcctg
    24361 aacaatgtgg aaaccttcgc caacatcccc cagatcatgc tccacggagc caagtggttc
    24421 aacagcctgg gaacggagaa gagcaaaggg accaaggtct tttcccttgc cgggtgcgtg
    24481 aacaacatcg gcctggtgga agttcccatg ggaatttctc tccgttccat cgttttcgac
    24541 atcggggacg ggatcaaggg atcgaataaa ttcaaggccg cccagctggg agggccttcg
    24601 ggcggatttg ttccggagaa cctgctggac accccggtgg actacgaatc cctggaggct
    24661 acaggggcca tcatgggctc gggcgggatc gtcatcttgg acgaagccgc ctgcatggtg
    24721 gactcggcgc ggttcttcac cgatttctgc gtggatgagt cctgcggcaa gtgcgtcccc
    24781 tgccgtaccg gtcttgcggt gatgctccgc aaactcgaag acatcgtggg gggaaaaggc
    24841 caggaaggag acgtggagtt tctggaggac ctgggacagc atatcaagag gatgtcccat
    24901 tgcggcctgg ggcagtcggc ccccaacccg gtgctctcca ccatccgcca cttccggcac
    24961 gagtacgaag agcacatccg ggacaaacgt tgtcgctccc tggtctgccg ggccctgatc
    25021 cgcttccggg tgaacgaaga gaagtgcaag atgtgcgggc tctgcctgaa ggcctgcccc
    25081 gtgggcgcgg tgacctggga gaggaaaaag ccggccaaga tcaatctgga cctgtgcacc
    25141 aaatgcaagt cctgcatccg ggcgtgtaaa ttctgggcga ttgaataagc ggtcagcaat
    25201 cagaaaagaa tgtggttttc gctgaccgct gatagaggtt atggaatggc taaagaacaa
    25261 aagatggtaa cgctcaccat tgacggggtg caggtctcgg tccctcctgg gaccatgatc
    25321 gtggaggccg cggccaaagc ggggatcaag atccccaccc tctgcaacaa taagcggctc
    25381 ctgccctacg gggcctgccg catgtgcgtg gtccagcaga aggggcgccg gggatttctc
    25441 atgggctgtt tcaaccccgt ccggaacgcg atggagattc taaccaacac cccggagatc
    25501 atcaaagccc gccgcatcca gctccagctc atcctcatct cccatcccct ggactgcccg
    25561 gtctgcgatg ccgggggctg gtgcgacctg caaaacctgg cctatgagta cggggtcgcg
    25621 gagaaccctt tcaaggggaa aaagaccgat cttcccgtcg atcatgtctc cccctttatc
    25681 gagcggtacc cgaaccgctg cattctttgc ggcatgtgcg tgcggatctg cgacgaagtg
    25741 gtgggagcga gcgagctttc cttcgtcaat cgggggatga aaacccggat cactaccgac
    25801 ctcgaccggc ccttgaactg tgaattctgc ggccaatgtg tctccgtctg cccggtgggc
    25861 gccctgaacg accggatctt gctccacaaa gcccgggtct gggacctgaa ggacaccctt
    25921 acagtatgcg cctactgcgg tgtcggctgc accctgctgg tggggacaag ggacaacaag
    25981 gtcatgcgcg tccgggccga tgaggacctg ggcctgaacc aggggaacct gtgcgtgaag
    26041 ggccggtttg gttgggagta catccacaac ccgaaacggc tgaccgcgcc cctaatccgt
    26101 aaaaacggag agccggtcaa agccacctgg gaggatgctc tgggtctggc agcccgccgc
    26161 ctgaaagaaa tcaaggcgga gaagggcgga tcggccctcg ccggcttggc ctccccgcgg
    26221 ctcaccaacg aagaactcta tcttttccag aaattctgcc ggggggtctt ggggacgaac
    26281 catctcgacc atgccggcgg ttacagttac gccgctcact tggccctccg aaattccctg
    26341 ggatatgccg cttccaccaa ctcgatcaac gaggtccgaa aggccgatgt catcttgctt
    26401 ctgcggtccg acctctccga aacccacccg gtgatcaagt ccgaggtagt cctggcggca
    26461 aagcgccgga aggccaaagt catcatcgtg aacagccgca acatctacct caaaaaattc
    26521 tccaccctca gcctgacggt gacaccgggg aacgaggtgg ccctggtcaa tgggatgatg
    26581 gcggtcatcc tgaaggaagg cctaacccag gaagatttcc ttcaatcccg cacccaggga
    26641 ctggcggcct tgaaaaagaa cttggaatcc ttttccctcc agaaggtgga ggctcagacc
    26701 ggggtggcgg ccgattctct ggcagaagcg gcccgcctgt acgccaaggc caagaccggg
    26761 gtcatcctga tttccacggg gcagaattcc gggcaagagg actcggccct ggcccaggcc
    26821 gccgccaatc tggcccttct caccgggaag atcggaaggg agaactgcgg ggtctttgtg
    26881 cttggggaaa agaacaattc ccagggcgcc ctggacatgg gcgtgactcc cggcctgctc
    26941 ccgggctatg ccgatctgag ggatactgcc gagcgctccc ggttcgagaa agcctggaat
    27001 ctgacccttc ctgatcagga aggaaggggg gccctggcca tccttcaggg catcgaagag
    27061 gggaagatca agggtctcta cctggccggc gaaaaccctg cggttaccta tccggattcg
    27121 gagcggacga ggaaggccct tgcatcgctg gatttcctgg ttgttcagga ttgcttcttg
    27181 accgagaccg cctccctggc tcacgtggtc ctaccggctg taacctttgc ggagaaggag
    27241 ggaactttca ctaacgtgga ccgcagggtc cagcgggttc gccctgctct ggcggctctg
    27301 ggggaagccc ggtctgatct ctcgatcttc cagggtctgg cccaggaaat ggggaccccc
    27361 ttggggtcag gatcggccca agaggtgaac gatgagatgc gaagcctggt aaccctgtac
    27421 ggggggatcg gctttacccg cctcgattcc ccgggcgttt cggcaggaat tcaatggccc
    27481 tgcccttccg ccgatcatcc cggaacaccc attgtctacg agaaggaatt tccggtggag
    27541 aaagccaggt tggtttttac ggattaccaa gaagcaaaga aggaaacgga ttcctccttc
    27601 gcgctggcca ccgggccgac catgttccac tctggttctc tttccacctt gagcccgggg
    27661 ttagtccggt tgcaggggga cggcttcgtc ctggtccatc caacggatgc caagaatctc
    27721 ggcctgaccg aaggccagaa ggttaccctg gaatccccgc agggaagggt catggtgaaa
    27781 gtctccatct cccctagaac gggtcccggt gtcctattcg ttcccaccca tttcgcggaa
    27841 aacggcggga accggctgac cggctgggac ctgaagacag cccgcgtgaa actggagaaa
    27901 agctaatcct ctccctgggg aaaaaggaaa aggtatgctc atctatcttc tgatttcagt
    27961 ggtgaaggtc ctaatcgtct ttacggtcat gctccttatc gtggcctaca tgactctcat
    28021 ggaaagaaag gttctgggcc acatgcaggt tcgttttggg cccaaccggg tcgggccctt
    28081 cggcctgctc cagccagtgg ccgacgggat caagctcttc ttcaaggaag acatcatcgt
    28141 tcccttcgcc aaccgaatca tttacatcct ggccccggcg gtgatcgtca tcaccgctct
    28201 ggtgagtttc gccgtcatcc ctttcgggaa cagcatcaaa atcatgggcc agagcatcga
    28261 cttggtggtg gccgatgtga atgtgggtct cctctatctc tttgccgtct cctccctggg
    28321 ggtgtatggg gtggttatgg gggggtgggc ctcgaacaac aaatattctc tcctggggtc
    28381 catccggtct tcggcccaga tgatcagtta cgaactgtcc ctcggccttt ccatcgtagg
    28441 ggtgttgatg atcgtcgggt cgctctccct caccaagatc gtggaagccc agtcttcggt
    28501 ctggttcatc ctctgccagc cgttggggtt catcattttt ctcatctccg ccgtggccga
    28561 gtgcagccgg acccccttcg acctgaccga atgcgagaac gagctcgtgg ccggctatca
    28621 gaccgagtac agctccatga agttcggcct gtactacctg ggcgagtatg cccacatcct
    28681 ggtggtgagt tctctggccg tatccctctt cttgggggga tggcagggac cttttctgcc
    28741 cccagtgatc tggtttttga tcaaagtctt cgccttcatc tttttcttca tctggattcg
    28801 ggccaccttc ccccgtttcc ggtatgacca gctgatgaaa ttcggatgga aagttctctt
    28861 ccccctgtcc ctggtgaaca tcctggttac cgccgtgttc atcatccttt tcttcaaata
    28921 gaggcgggaa gaaccttttg cgaggaaccc tatgatactg ccgttgctga aaggattgaa
    28981 attaaccttc cggactttct ttacccgtcc catcaccatc cagtacccgg agcagaagat
    29041 ggtcatgttc ccccgcttcc gggggctgca cgaactggcc cgggacccgg aggggatgat
    29101 catctgtgtg gcctgtgagc tctgcgccgc cgtgtgcccg gccaactgca tccgggtgca
    29161 gccgagcgaa ggcccgaccc ataaacggtt ccccaaggtc tatgagatcg acctgggccg
    29221 ctgcattttc tgcggcttct gccaggaagc ctgcccctac ggggcgattc tccttaggga
    29281 caactacgaa atcgccaact atacgcggaa ggacctggtc tacaaccgcg accaactcct
    29341 ccacgcctac gaccggaaat agggaggccc gttccatgct ggagactatc ctctttgtca
    29401 tttttgcggg gatcgccgtg gtaaccgccg tcctggtggt cgttcagcga aatcccgtga
    29461 gcagcgccat ctttctcatt ctcacttttt tcgctctggc cggaatctac cttctgctcc
    29521 acgccgagtt catcgccgcc atccaaatca ttgtgtacgc gggggccatc atggtcctct
    29581 ttctcttcgt gatcatgctc ttgaacctgg aaaaagaaaa gcgcctcgta gcccgcaacc
    29641 ggctgctgaa aggggtgggg gttttcctcg gcgtggtcct gctggtccag atcgggatgg
    29701 tcctgcagcg ggtccttctg gagggaaccc cgggaaagtt ccccccggag aaggtcaccg
    29761 ccctgggaaa cacccaggtc gtggcccagc ttctcttcac cgattttctt cttcccttcg
    29821 agatcacctc ggtgctgctc ctggtggcca tcatcggggc gattgtcctg gccaaacgcc
    29881 aaatttagag aaggagcgga aatggtctct ctttccagtt atctgatctt gagcgcgatc
    29941 cttttcagta tcggcgtggt ggggttcctg ctgcggcgca acaccatcgt gatcttcatg
    30001 tctatcgagc tcatgctcaa cgcggccaac ctggccttcg tggccttctc ccgttttctc
    30061 aattccatgg acggccaggt tattgtggtc tttgtcatgt ccgtggccgc agccgaggcg
    30121 gcggtggggc tggccatcat catggccatg taccgcaccc agaagacggt caacgccgac
    30181 gagatgaatt tgatgaaatg gtgattccgg ttcctggttg cttgttcctg gttactcgtt
    30241 caaatgccct ggaacgttta acgataaccg ataaccggca accagtaacc agcaacgagc
    30301 aaccagtaac gagtaaggag atgcgggatg cttgattatg tgggtctgat accattgttt
    30361 ccggccatcg ggttcgccat caatctcttc tttgggaggc ggatctccaa gggcgccgtg
    30421 ggcttcattg cctgcgctgc catcgggctc tcttttctcg tctccctcct ggtcttctct
    30481 gccctcctga agctgcccgt ggccggccgc tccgtggaaa gaatcattta cccctggatt
    30541 ctctccggcg agttccaggc gaatatcggc ttcctggccg accccctctc cctggtcatg
    30601 atgctggtgg tttccggcgt ctccttggtg atccatattt attccgtggg gtacatgcac
    30661 gacgacccgg actatccccg atacttcacc tacctcaatc tcttcgtatt cttcatgctc
    30721 attctggtgt cggccaacaa cttcctcctc atgttcgtcg ggtgggaagg ggtggggctt
    30781 tgttcctact tcctcatcgg gttctggtat gagaagaaat cggcctccga tgccggcaaa
    30841 aaggccttca tcgtcaaccg gatcggggac ttcggattcc tcctggccat cttcctgatc
    30901 ttcgtgacct tcgggactgt cgatttcacc aaggtctttg ctgcggctcc gggaaagctg
    30961 gaggtgggga gcacgctggt aaccttaatc accctcctcc tcttcatggg ggccaccggg
    31021 aaatcggctc agatccccct ctacacctgg cttccggacg ccatggaagg cccaacccca
    31081 gtcagcgccc tgatccacgc cgccaccatg gtgaccgccg gggtttacat ggtggcccgg
    31141 tgcaacgttc tcttcatcct ggcccccacc tcgatcctga tcgtggccat catcggcgca
    31201 gccacggcca tcttcgccgc ctccatcggc ctggcccaga atgacatcaa aagggtcctg
    31261 gcctactcca ccatcagcca gctgggctac atgttcctgg cctgcggggt gggagctttc
    31321 accgccggca tttttcacct catgacccat gccttcttca aggccctcct cttcctggga
    31381 gcaggcagtg tgatgcacgc catggccggg gagctggaca tgcgaaaaat gggcgccctg
    31441 aagccccaca tgccccgcac ctactggact tttctcattg ccaccctggc catcgccggg
    31501 atcttcccct ttgccggatt tttctccaag gatgagatcc tctggaaggc cttgacccag
    31561 ggcggggtgg tcttctggct catcggggcg gtcgccgcct tcatgaccgc cacctacatg
    31621 ttccgagctg ttttcatgac ctttcacggc agctcccggg tggaccctca tgtggcccat
    31681 cacctccacg agtccccggg gatgatgacc gttcccctga taatcctggc catcctttcg
    31741 gtggtggggg gattcatcgg tttccccatc gtggaaggat ggaacaaatt caatgaattc
    31801 ctcgccccgg tctttgccaa gggcgtgcct gccgaagggg cccaccacgc ggtcagcttc
    31861 gaggtggcca tgatgctggt ttccatggcc attgccggtt tggggatcct cctggccttc
    31921 aagatgtata tcaagaatcc tcggctgccc gatcagatgg tcgagcgata ccccacgcct
    31981 tattctctga tcgccaacaa atactgggtg gatgagattt ataactgggt tttcgtgggc
    32041 cctctgattc ggttctccgt cttcctgtgg aggattatcg acgacatcct ggtggacggc
    32101 acggtcaatg gcgtggccgc tgtagcccgg ggcggaagcg aagttttcaa acgtttacag
    32161 accggaaaca tccagggtta tggcctatcc atccttgtag gggtggtatt cctggtcggg
    32221 tatctattaa tctacacgaa atagataaat ttacataatg tgtagggtgc gttccccgaa
    32281 cgcaccgtct gacggcctga tcggggatca ggccctacaa aaaaggagcc gttttcatga
    32341 gcagcctggc aggaattccc cttctcacgt tgattacctt cttccccatg gtgggagtct
    32401 tggtcctcct ctttttggac aaggagaaga aggacacgat ccgccaggtc accctcgggg
    32461 tgtccctgct ggaatttctc ttctcccttc ccctcttctt cagcttccgg ctggacacgg
    32521 cggccatgca gttcgtggaa aaggttcc
//
LOCUS       DASPRR010000430         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2195092, whole genome shotgun sequence.
ACCESSION   DASPRR010000430 DASPRR010000000
VERSION     DASPRR010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195092"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(564..1085)
                     /gene="folK"
                     /locus_tag="VEL68_15795"
     CDS             complement(564..1085)
                     /gene="folK"
                     /locus_tag="VEL68_15795"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXZ37484.1"
                     /translation="MLAYIGIGSNLGDKPENCRRAIEALGSDPRNLLVRCSPLYRTEP
                     VGKTDQDWFVNGVVSLETSMDPRELLEFLLSIERKMGRVRGEKWGPRTIDLDILFCGD
                     QILNETDLQVPHPRLHQRRFVLIPLQDVAPDLVHPVLGKTVSRILAELDTRERVIPLQ
                     EGNEKACTVSSSF"
     gene            complement(1205..2374)
                     /locus_tag="VEL68_15800"
     CDS             complement(1205..2374)
                     /locus_tag="VEL68_15800"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HXZ37485.1"
                     /translation="MQVADRLQKIPPYLFVTLRNKINKARAEGIDVISLGVGDPVDPT
                     PQSVIDELCRQAQIPVNHQYPIDEERGMLAFRKEVARWYARRYRVELNPEGEVVGLIG
                     SKEGCHHFILGVVNPGDVVLMTDPGYPAYRASIFMANAEPYGMPMLAENDYFPDFDQI
                     PKDVLKRAKAMFLNYPNNPTGACATREFFKKAVEFAKANDIAICHDNPYSEVLFDGQE
                     RLSILSVPGAKEVAIELNSLSKPYNMTGWRIGMALGNKGILAALLKVKENTDSGIFNA
                     IQYAAIVALREEDKNIEKMLGIYLRRRKLVLETLAKIGIEFKPQKGTFYLWIPVPRGM
                     TSLDFTTALFEKAAVVVAAGTAYGKHGEGYVRISLTVPDKRLEEAMERIQKALGK"
     gene            complement(2557..>2878)
                     /locus_tag="VEL68_15805"
     CDS             complement(2557..>2878)
                     /locus_tag="VEL68_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37486.1"
                     /translation="YHFKYSMGLPGKIVPVTSSKVVTEIQAFALMAGVRAYHIASGGV
                     GGSEGAVALALQGEEKKVEKAFELAKSVKGEPPVTMPTQFKVGSPADYQYDALKQLNT
                     LGGF"
BASE COUNT          640 a          765 c          747 g          726 t
ORIGIN      
        1 tcgacgtatt tatagatatc agcccatgaa tctgaggaag cgttaagacc caggacccca
       61 aaaccgaaaa gaaaagccaa ccaaatcttc ccgggtctcg ggagtcgatt cttttccttc
      121 cgattcaccc ttttgcgccc ctgcataaaa atctacctct cgtaagtttt tatacaatag
      181 cttatcgact ttgaagatgc aatcattaaa tcgccggcga gccttaaaat cgtccccaag
      241 aggcaggatt cacctgtcct aatttgtctc tctctttcca aaaagataaa taaaaaaggg
      301 aagtttggat gatctaccgg agctgaaagg gggtcaattt ttggggaggg cgttggagga
      361 ctggtattta tgagagcatg cctggctgca gaaatacagc tttttccctc ggtgtgaaag
      421 ggtgaatgcc tggcttttgg gaacgtagca cccgcaaatt gggtcctgga tcatctcctc
      481 gccaatttcc tgggaccctc caggtctact tttcggggag aaaaaagccc gcttgaccat
      541 ccagtagagg atgcccatca aaatcagaat gaagatgaaa cggtacatgc tttttcattt
      601 ccttcctgga ggggaatgac cctctctcgg gtatccagct cggcaaggat cctggacact
      661 gttttgccca gtacaggatg gaccaaatcc ggggcgacat cctgtaaggg aatgaggaca
      721 aatcttcttt gatgaagtcg gggatgggga acctgcaaat cggtttcatt caggatttga
      781 tcgccacaaa ataggatatc taaatcgatc gtcctgggtc cccacttctc tcctcgaacc
      841 ctccccatct ttctttcgat ggaaagaaga aactccaata gctctctggg gtccatcgag
      901 gtttctaaag aaacgactcc atttacaaac cagtcctggt ccgttttccc caccggttcc
      961 gtgcgataga gtggcgaaca tcgaaccagg aggtttctcg ggtccgaacc cagggcttcg
     1021 atcgcccggc ggcagttttc cggcttatcg cctagattcg aaccgattcc gatatacgct
     1081 aacataaaaa cgtttcgagt cctgggtttc gggtctcaag tttcaagttc aactggttgc
     1141 ctgctgctgg ctgctcgcta ctgctctagc ctatttaccc gggactcaaa acccgaaacc
     1201 cgttttattt ccccaatgct ttttggatcc tctccatggc ctcttccagg cgcttgtcgg
     1261 ggacggtgag ggaaatgcgg acgtatcctt ctccatgttt gccgtaggcc gttccggcgg
     1321 cgacaacgac agcggccttc tcaaaaaggg ccgtggtgaa atccagggag gtcatgcccc
     1381 tggggaccgg aatccatagg tagaaggtcc ccttctgggg tttgaattca atcccgatct
     1441 tggccagcgt ttccagaacc agcttgcggc ggcgcaggta aatcccaagc atcttctcaa
     1501 tgttcttgtc ctcttcccgc agggctacga tggctgcata ctggatggcg ttaaagatcc
     1561 cggagtcggt gttctctttg accttcaaca aagcggccag gatgccttta ttgcccaggg
     1621 ccatgccgat gcgccagccg gtcatgttgt agggcttgga gagggagttc agctcgatgg
     1681 ctacctcttt ggcccctgga accgaaagga tgctcaaccg ctcctggccg tcaaagagga
     1741 cttcgctgta cgggttatcg tggcagatgg cgatgtcatt cgccttggcg aattcgactg
     1801 ctttcttgaa gaattcgcgc gtggcacagg ctccggtggg gttgttgggg tagttgagga
     1861 acatggcctt ggcccgtttc agcacatctt tgggaatctg gtcgaaatcg gggaaataat
     1921 cgttttccgc cagcatgggc atgccgtagg gctcggcgtt ggccatgaaa atgctggccc
     1981 ggtaggctgg gtatccggga tcggtcatca atactacgtc tcctggattc accaccccaa
     2041 ggataaaatg gtgacaaccc tccttggagc caatcaaccc caccacttcc ccctcagggt
     2101 tcagttcgac tctgtatctt ctggcatacc atcgggccac ttccttgcgg aaggccagca
     2161 ttcccctttc ctcgtcgatg ggatactggt gattgaccgg aatctgggcc tggcggcaga
     2221 gctcgtcgat cacgctctgg ggggtcggat cgacgggatc tcccacgccc aggctgatca
     2281 cgtcgattcc ctcggcccgg gccttattga tcttgttgcg cagtgtaacg aatagatagg
     2341 gcggaatttt ctgtaaacgg tcggcaacct gcacgttctc ttcctcctga actttttttc
     2401 gggtaaggat tttctaacat aagtcggggg gaggcgcaag ggaaatgcgg aaaaccagaa
     2461 gtcaggagcc aggagtcaga agtcaggaga aactacccac gagataaatt ctttttctct
     2521 cttgacttct ggattctggc tcctggattc cgaaatttaa aacccgccaa gggtatttag
     2581 ctgttttagg gcatcgtact gataatcagc cggcgagccg actttgaatt gtgtgggcat
     2641 ggttaccgga ggctctcctt tgaccgactt ggccagctca aaggccttct ccactttctt
     2701 ttcctcccct tgcagcgcga gagccactgc cccttccgaa cctcccactc cgcccgaagc
     2761 gatgtggtaa gcccggactc cggccataag agcaaaggcc tgaatttccg tgaccacctt
     2821 gctgctggtg accgggacaa ttttaccggg aaggcccatg ctgtatttga aatggtag
//
LOCUS       DASPRR010000431         7200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2038662, whole genome shotgun sequence.
ACCESSION   DASPRR010000431 DASPRR010000000
VERSION     DASPRR010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038662"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VEL68_15810"
     CDS             complement(<1..1811)
                     /locus_tag="VEL68_15810"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ37487.1"
                     /translation="MVSKTSSLAKLARPVLTRVFLRERPFDLLDRLRQQPVIWISGPA
                     GCGKTTLASSYIETRRLPCLWYRLDRGDGDAATFFYYLALAARKASPRVKTPLPLLTP
                     EYLQGLSTFTLRYFENLYQRLRRPAILVFDNYQEVPSEASFHDIILQGLSNTPDGINI
                     FLISRQDPPAGLVRLGANHQMAFLPWGELRLTLEESTGIAAVRFPEKLPPKSLHQLHS
                     TADGWAAGLILLLERLKREKIDFKQLGKLVREEIFDYFSNEILNPLDPETRDFLLKTA
                     LLPQMTESMAERLTGQPQAGRTLARLNRGNYFMEKRILEEPIYGYHPLFREFLLSQGE
                     KTFSEENLRFFIGQAAEILEEHGQVESAVRLLQKIGDWESLVRLILKHAPVIHAQGRY
                     SVLEGWLRSLPPGLVEARPWLTYWMGSCCMFADPARSRPHFEQAYRQMRKERDAAGLF
                     MAWSGMMDDINLGYGFEPFSALDRHLQELEEIRRDYPVFPSAEIEARVASAVVAALAL
                     RQPNHPEFEAWAERALRLAQDQTVVNMRIQSLFFLAYYHLYGGNREKALLAINQLRQM
                     SQSPQASAYTKVMTKMAEAMHYCIAGLSRESMKAAQTG"
     gene            complement(1838..3352)
                     /locus_tag="VEL68_15815"
     CDS             complement(1838..3352)
                     /locus_tag="VEL68_15815"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXZ37488.1"
                     /translation="MPIQDFTPDKLIRAAADLGLIISGHEAESYCRILTPWVSAYKAL
                     DAIPDSPPTPIYPRAPGYRPEGEANKYNAWYYRTSIKGATKGPLKGKTVALKDNIMLT
                     GAPMMNGASTLQGYVCDRDATVVTRILEAGGEIAGKVHCEWFCMAASSFTSALGPVHN
                     PFKMGYSAGGSSSGCAVVVAIGDVDMAIGCDQGGSIRIPSSFCGIYGMKPTHGLVPYT
                     GILSLDPQIDHVGPMTRSVRDNALLLEAIAGPDGHDSRQMKVVTHPYSQMLEGGVAEL
                     RIGVLREGFGHPNSEADVDAKVRTAAQLFKKMGALVDEVSVPMHLMAGAIFTPIAVEG
                     ALHTWAAGDSVGIGRPDVYATSLMDYHRGWAQHLDPMPPLLKALFMLESCMRKDFGHR
                     YYGKACNLIPSLTRAYDTILNSYDLLLMPTTPIKASKLPGPEASLEERAERSVDMVAN
                     TLPFNLTHHPAMNVPCGMSDGLPVGMMLVGRCHAEPTIYRAAYAFEQAQDWKAA"
     gene            complement(3674..4090)
                     /locus_tag="VEL68_15820"
     CDS             complement(3674..4090)
                     /locus_tag="VEL68_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ37489.1"
                     /translation="MIVSLREDSDPSPDFYFHHQFAIYHESYLIWDRGTWEMVLATCA
                     VYRVEVDGNYAGDVILEGRPKGTRYIVDFSLLPEYQGKGIGTAVLEEVKKMAKKLTAI
                     TRKEKLPFFLKCGFALRKTVKNYYDSGVDGYHIVFG"
     gene            complement(4087..4971)
                     /locus_tag="VEL68_15825"
     CDS             complement(4087..4971)
                     /locus_tag="VEL68_15825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXZ37490.1"
                     /translation="MILILLLLFLAAPFFVSPEKAVGGETPLQVEVLPRSVKQGEVCL
                     LKAWGPGSLKSVHGEFQGRRYPLGSEDQYQTFEGLLGVDLQAKPEKYSIRFMTTDANG
                     QVDSKTFSIKVEKADFGVQKLTLPRSMVDLDPQTLERVNQESKRLKELFQKFWNEKTW
                     KGAFIRPVEGELSGAFGTRRIINGQPKSSHTGVDLRAEEGTPVWASNSGRVALVDELF
                     FTGKSVILDHGWGLYSMYFHLSEVRVQEGDRIPKGAQLGRVGSTGRSTGPHLHWGIIL
                     NGARVDPLSLLRSTASLQ"
     gene            complement(4968..5753)
                     /locus_tag="VEL68_15830"
     CDS             complement(4968..5753)
                     /locus_tag="VEL68_15830"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HXZ37491.1"
                     /translation="MSKFDVLLQDLNTYLKLQSNPVAVKMLRKGEKPPVPAKFPLKDF
                     KKRMVLCQAWTIAKRHEETVCMRYEDMRCAPSVLAMGFKPPIPYFTEGHLCIDRYSKT
                     LEAGARSEALVPMFLHGTYDAVLFSSLTTCNFDPDAIFLSGNSAQIMRLIHAALYREG
                     GRISYSTDGRLSCADLIVTPIQNPGFYMVLPGHGSRRLAQAKDDEMAFTFTCTPEKVD
                     QLREGLARTHEAGLRYPVRIHLDYEVVFPGQYDQLFTLWEKEK"
     gene            6161..7033
                     /locus_tag="VEL68_15835"
     CDS             6161..7033
                     /locus_tag="VEL68_15835"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HXZ37492.1"
                     /translation="MSAARFLIIFLILGTFAFNTGCISSQKIVAEKAPALFRDVAQSA
                     NRQSDIVLVRQGIPSYLMLIDGILQSNSENRDLLLAGAQAYAAYASVLDEDEQDRAAA
                     LSDRAKQYALKALDLTPPFKDALGKPLEVFQERLGKTDQKQVPTLFWVGNIWAGWIAS
                     NEGAAAMADLPWVEALMERVLQLDPGFYYGGPHLFKAILLSARPEQFGGNLKKAQVHF
                     QKALDYGQGKFLTTEVYYAEYYARQTLNRDLFVSTLKRVLETPAQVEPDLTLANTLAQ
                     RKAKKLLARVDEFF"
BASE COUNT         1602 a         1998 c         1963 g         1637 t
ORIGIN      
        1 ccggtttggg ccgctttcat actctccctg ctcaaaccag cgatgcagta atgcatagcc
       61 tcggccattt tcgtcatgac cttggtgtaa gctgaagctt gcggagattg gctcatttgc
      121 ctcagctgat tgatagcgag caacgccttt tcccgattcc ccccgtaaag atgatagtag
      181 gcgaggaaga aaagggactg aattctcatg ttgaccacag tttggtcttg ggccaggcgc
      241 agggctctct cggcccaagc ctcaaattcg ggatgattgg gctggcggag ggcgagcgcc
      301 gcaacaaccg ctgaggcgac tcgggcctcg atttccgcag aagggaaaac cgggtaatcc
      361 cgtcggattt cttccagctc ctggaggtgg cggtcgaggg cactaaacgg ttcaaaccca
      421 taccccagat tgatatcatc catcataccc gaccaggcca taaaaaggcc ggcggcgtcc
      481 ctttcttttc tcatttgccg gtaggcctgc tcaaagtgag gccggctccg ggctgggtcg
      541 gcaaacatac agcaggaccc catccagtag gtaagccagg ggcgggcttc gaccaatcca
      601 ggagggaggc ttcgcagcca tccctccaaa accgaatacc tgccctgggc atgaatcacg
      661 ggggcatgtt tgaggatcaa gcggactagg ctttcccaat ccccgatttt ctgtaggagg
      721 cgaaccgcgc tctccacctg cccgtgctcc tccagaatct cagccgcttg gcctataaag
      781 aagcggaggt tctcttcgga gaaggttttt tcaccctgag agaggagaaa ttcccgaaat
      841 agaggatggt agccgtaaat gggctcctca aggattcgtt tctccataaa atagttcccc
      901 cggttcagcc gggcgagcgt ccgcccagcc tgaggctgtc cggtaagcct ttcagccatg
      961 ctttcagtca tctgggggag aagggctgtc ttcagcagaa aatctcgggt ttccggatcc
     1021 aggggattca agatttcatt tgaaaaatag tcgaagatct cttcccgaac caatttcccg
     1081 agctgcttga aatctatctt ttcgcgcttc aatcgctcca gaaggagaat caacccggct
     1141 gcccatccat ctgctgtact gtgaagctga tgaaggctct ttgggggtaa tttctccggg
     1201 aatcgcacgg cggcgatccc tgtggattcc tccagcgtaa gacgaagctc accccatggg
     1261 agaaaagcca tctgatgatt cgctcctagg cggaccaatc ccgcaggggg atcctggcgg
     1321 ctgatcagga aaatgttgat gccgtccgga gtgttggata ggccttgaag aatgatgtca
     1381 tggaacgatg cttcggacgg cacctcctgg taattgtcga agacaagaat agcggggcgc
     1441 ctcagcctct ggtagagatt ttcgaaatac cgcagcgtaa aagttgataa tccttggaga
     1501 tattctggag tgagtagagg aagcggcgtc ttcacccgag gggaagcctt cctggcagcc
     1561 agggccagat aatagaaaaa agtagctgca tccccatctc ctctgtcgag ccggtaccac
     1621 aaacagggga gccgccgggt ttcgatgtag ctcgaggcca aagtggtctt gccgcacccc
     1681 gcaggcccgg agatccagat caccggttgc tggcgcaggc gatccaagag gtcaaagggc
     1741 cgctcccgca ggaacacccg ggtgagaacg ggacgggcta atttggccag cgaggaggtt
     1801 tttgagacca tggggtatta gaaaatttcc ccaggcttca agccgccttc cagtcctggg
     1861 cctgttcgaa ggcgtaagcg gcacggtaga tagtcggctc ggcgtgacat cggccgacca
     1921 gcatcattcc cacgggcaag ccgtcgctca ttccgcaggg aacattcata gctggatgat
     1981 gggtcaggtt aaaaggcagg gtgttggcca ccatatctac gctgcgttcg gcccgctctt
     2041 ccaggctggc ttccgggcca gggagcttcg aggcttttat aggggttgtg ggcatgagca
     2101 gcaggtcata ggaattcaag atggtgtcat aagccctggt cagtgaaggt atcaggttgc
     2161 atgcctttcc atagtatcgg tggccgaaat cttttcgcat acaactttct agcatgaaca
     2221 gagctttcaa gaggggaggc atggggtcga gatgctgggc ccagccccga tgataatcca
     2281 tcaggctggt agcgtagacg tcggggcggc cgatccccac gctatcgccc gcggcccagg
     2341 tgtgtagagc gccctctacc gcaatcgggg tgaagatagc gcctgccatc aggtgcatgg
     2401 gaacagaaac ctcatcgacc agcgcgccca tctttttaaa gagctgggct gccgttcgaa
     2461 ccttagcatc tacatctgct tcagaattag gatgaccgaa tccctccctc agaacaccga
     2521 ttcgcaactc ggcgacgcct ccttcgagca tttgactgta gggatgcgtg actaccttca
     2581 tctgccttga gtcgtggccg tcgggtccgg caatcgcctc cagcagcaaa gcattgtcac
     2641 gaacactgcg ggtcatcggc ccgacatggt cgatctgcgg atcaagcgat agaattcctg
     2701 tataaggcac cagcccatgg gtgggtttca tgccgtaaat tccgcagaat gacgagggga
     2761 tccgaatcga accgccctgg tcacagccaa tcgccatatc cacatcgccg atggccacta
     2821 ccactgcaca tccggacgac gatccacctg ccgagtaacc catcttgaag ggattatgca
     2881 cgggccctaa ggcgcttgtg aaactgcttg ccgccatgca aaaccactca caatgaacct
     2941 tgcctgcgat ctcgcccccc gcttccagga ttcgcgtgac gacggtggca tctcgatcac
     3001 aaacataccc ctgcaaagtt gacgctccgt tcatcatggg tgcaccagtc aacatgatgt
     3061 tatctttgag agcgacggtt tttcctttca gcggtccttt ggtggcgcct ttgatggagg
     3121 tcctgtagta ccaagcgttg tacttattcg cttcaccttc cggtcggtat cccggcgcgc
     3181 ggggatatat cggcgtagga ggggaatcgg ggatggcgtc cagagccttg tacgcactta
     3241 cccatggggt gagaattctg cagtaagatt cagcctcatg accggaaatt atgagaccta
     3301 aatccgcggc ggctcttatc aacttgtctg gagtgaaatc ttgaatgggc atctgaattc
     3361 cctccgagct gggcgaactc ttgggagaac tgaagaagaa accttgggtt gaaacctaag
     3421 gtattattat gctttaccgg gccgggcagc cctacgaatg gaatggaacc ctcgcctgag
     3481 ttgagcggca cgcccagaca atcaccagtt ctcagctgag ccgtatctaa ccgcggtcat
     3541 tcattttatc gattggaatt ttaccggaac agctgcaggg tgtcaataaa cagccaccgc
     3601 ctcagctgaa agttctggac ccaggcattc gccggggtga ggcttcaaga gacttttttc
     3661 gaaatcatca attttagccg aaaacaatgt gatagccgtc cacacctgaa tcataataat
     3721 ttttcactgt ttttctcaag gcgaacccgc atttcaggaa aaagggaagt ttctcctttc
     3781 gggtgatggc ggtcaatttc tttgccatct tcttgacttc ttccaggacc gccgttccaa
     3841 ttcctttacc ctgatattca ggtaagaggc tgaaatcgac aatgtatcgg gtgcctttgg
     3901 gcctcccctc caggatgaca tccccggcgt aattgccatc cacttccacc cgataaaccg
     3961 cgcaagtggc cagcaccatc tcccacgttc cccggtccca gatcaagtag gattcgtgat
     4021 agatggcaaa ctgatggtgg aagtaaaaat cggggctggg atcggagtct tcccgcaggc
     4081 tgacaatcat tgaagcgacg cggttgatcg gaggagggaa agagggtcaa ccctcgctcc
     4141 attgaggata attccccagt gaaggtgagg gccggtggac ctccctgtag accccacccg
     4201 gcccagttgg gcccccttag ggatgcgatc tccttcctgg accctcacct ccgagaggtg
     4261 gaaatacatg gagtagaggc cccatccatg gtccagaatg actgatttcc cggtgaagaa
     4321 aagctcgtcg accagggcaa cccttccact gttgctggcc catacaggag ttccctcctc
     4381 agcccgcagg tcaacgccgg tatgggaact cttgggctgc ccgttgatga tcctacgcgt
     4441 tccaaaagcc ccggacagtt ctccctccac cggacggata aaagcaccct tccaggtctt
     4501 ttcattccag aacttctgga ataattcctt cagccgtttt gactcctggt tcacccgctc
     4561 gagggtttgg gggtccaaat ccaccataga ccgcggtaga gttaatttct gcactccgaa
     4621 atcggccttc tccactttga tcgaaaaagt cttcgaatca acctggccgt tcgcgtccgt
     4681 ggtcatgaat cggatgctgt acttttccgg cttggcctgc agatcgactc cgaggaggcc
     4741 ctcaaaggtt tgatactgat cctccgatcc caggggatat cgccttccct gaaactctcc
     4801 atggacggac ttgagcgatc caggacccca ggctttcagg aggcacacct ctccctgttt
     4861 gaccgacctc gggagaacct caacctgaag aggagtttcc cctccaacag ctttctccgg
     4921 cgatacaaaa aaaggggccg ccaaaaaaag aaggagaagg atcagaatca tttttctttt
     4981 tcccaaaggg taaacagttg atcgtactgt ccggggaaga ctacttcgta atccaggtga
     5041 atgcggaccg ggtaacggag gccggcttca tgggtcctgg ccaatccttc cctcaactga
     5101 tccacctttt cgggcgtgca ggtgaaggtg aaagccatct cgtcgtcctt cgcttgggcc
     5161 agccgcctgg agccgtgacc cggaagcacc atgtaaaatc ccggattctg gataggagtc
     5221 acgatgagat cggcgcagga taggcggccg tctgtagagt aactgatccg gcctccctcg
     5281 cggtagagtg cggcgtgaat gaggcgcatg atctgggccg aattcccgga aaggaaaatg
     5341 gcatcgggat cgaagttgca agtcgtgagc gacgagaaca gaacggcgtc gtaggtgccg
     5401 tggagaaaca tgggcaccaa ggcttcgctc cgcgcccctg cctccagcgt tttgctgtac
     5461 cggtcgatac acaggtgtcc ttcagtgaag tagggaatgg gaggtttaaa acccatggcc
     5521 aggacgctgg gcgcgcagcg catatcctcg tatctcatgc acaccgtctc ctcgtgccgt
     5581 ttggcgatgg tccaggcctg gcagaggacc atccttttct tgaaatcttt gagggggaat
     5641 ttggccggta ccggcggttt ctcacccttc cggagcatct tcaccgctac cggattggat
     5701 tgcagtttta agtaggtatt gaggtcctgc aggagtacat caaacttgct catttcggta
     5761 gcctccttgg caaaccagag tgtcagcgga aattgtgcct atatgtagga atcgccccta
     5821 taccattgcc gggaatgccg ctttcaaact tgtttcccgg atcaaggaag gattaatagt
     5881 gcaattattg ggcaaaagga gataattgtc aattcttcgg atggaccatt cttccttgcc
     5941 cacctccagt ttttcgtgct tgttggggtt cagggtggag aaatttggat catcaggctc
     6001 ggcgggtgct tatccttcca tgaaactcct cccaatgttc cgccccccct tctttccaga
     6061 gatcttattt tcgaaggact cttttttaag aacccggggt gggcaagacc attcttcccc
     6121 ttgacccggt cttttaatca gtgatacttt ttaattagac atgtctgctg cccgctttct
     6181 tatcattttt ctgattttgg gtactttcgc cttcaacacc ggctgcattt ccagtcagaa
     6241 gatcgtggcc gagaaggcgc cggccctctt tcgcgacgtg gctcaatccg ccaaccgcca
     6301 gtccgatatc gtccttgtcc gccaaggaat cccttcttac ctcatgctta tcgacgggat
     6361 tctccaaagc aattcggaaa acagggacct tctcctcgct ggagcccaag cctatgccgc
     6421 ctatgcttcc gtcttggacg aggacgaaca ggaccgggcg gctgccctga gcgatcgggc
     6481 caagcagtac gccctgaaag ccttggattt gacaccgccc ttcaaagacg ccctgggaaa
     6541 gccgctggaa gtcttccagg aacgtttggg gaagacggat caaaagcagg ttcccaccct
     6601 cttctgggtc ggcaatatct gggccggctg gatcgccagc aacgaggggg ccgcggcaat
     6661 ggccgacttg ccctgggtgg aagccctgat ggaaagagtc ctgcaactgg accccgggtt
     6721 ttattacgga ggaccgcatc tcttcaaggc aatcctcctc tccgccaggc cggagcagtt
     6781 cggcggaaat ctgaagaagg cccaggtcca tttccagaaa gccttggatt acggccaggg
     6841 caaatttctc acaaccgaag tgtactacgc cgagtactat gcgcggcaga ccctcaaccg
     6901 cgaccttttc gtctccaccc tgaaacgggt gctggaaacc cccgcccagg tggagccgga
     6961 cctgactctt gcgaacaccc tggcccagcg gaaggccaaa aagctcctgg cccgggtgga
     7021 tgaatttttc taggttgtta agaacggggg tgaaatggga ataagcagac ttctcggtgt
     7081 tgccatcctt ggcctgatcc ttttcctgtt cctggccggc gtgggaatag ccgctccagc
     7141 cgagcaagtg attttgaaga tggggaccct ggctccggag ggaacggcct gggtcaaggc
//
LOCUS       DASPRR010000432        14477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_756186, whole genome shotgun sequence.
ACCESSION   DASPRR010000432 DASPRR010000000
VERSION     DASPRR010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756186"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VEL68_15840"
     CDS             <1..1128
                     /locus_tag="VEL68_15840"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HXZ37493.1"
                     /translation="YMRARAYVSEELRKMGARVSTTVGGNLCGRLEGSIRGIPSVMTG
                     SHIDSVLRGGRFDGAAGVVAALEVARVMAEEKTPHRHPIDVVVFAEEEGSRFGSVMIG
                     SRAWIGKLSSEDLNRFKDADGTSYFAAMEKAGLNPGDGPLLRSGMVKGMIELHIEQSL
                     VLENKGFPIGVVEGINGIKQFLVTITGVPNHAGATPMGLRHDALQGAVRAIAAVEEIA
                     IQDLGANTVATVGMLTCDPGQANVIPGRVQFTVDVRDLDRERIDQAAQRILSVIQTIC
                     RARGLTFDIKPRSDTPPVRLSKELVQLIEETAQQKQVQALRMASGALHDSSILPEVTE
                     VGMIFVPSKAGRSHCPEEETDLRDLRTGADLLLGAVAKLAT"
     gene            complement(1203..2159)
                     /locus_tag="VEL68_15845"
     CDS             complement(1203..2159)
                     /locus_tag="VEL68_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37494.1"
                     /translation="MRTPLILIILLAAIGVFPIFFPSPYLIHIGTLIFIWSFITTAWS
                     YMGRFGLVSLGHGAFLGLGAYTSGLLFNFYGLSPWIGMLLGGVVAVLFAMLIGYACFR
                     FGVIGDYFALVTLALGEVVALTIIAFRDITGGSLGFTLKSKGTSLLYFQSEQKIHFYY
                     ISVAFLIIALLVWKWIDRSKMQKALRAIGEDEIAASTLGVGIIRYKMIITSISAFMTA
                     IGAVLYAQYITYLNPHTLSGVPPSLEIAFKAILGGMFSLWGPAVGTALIVSLEEYFRV
                     AYGSTFVGFSQIIYGLALVLLIIFLPKGIYGTLQELFKKKRA"
     gene            complement(2170..3042)
                     /locus_tag="VEL68_15850"
     CDS             complement(2170..3042)
                     /locus_tag="VEL68_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37495.1"
                     /translation="MLDLYLNAVINGILLGGVLALLAFGMNLIFGVVKIIHMAYGQCV
                     MLGLYFIYTLVTHLGFPILLACIATLPLMGVVGFGLQVLVINPLLRAKANPLTQLLAL
                     AGLTIVLENLAQVIWGADLRGINVQLPIISIGNVFLRTSNLIAFFGALLTLWGLYLFL
                     NRTYMGLAIRSIAQDLDSARLMGINPQAIYYLTMAAGGLLTALIAGFFSPIYAVHPHF
                     GGSFTMMAFIIVVLGGMGNLLGGFIGAFIIGIVTSVSAAMTSTEIADIVALVIFIAVI
                     LVRPQGLLGIRVAK"
     gene            complement(3046..3750)
                     /locus_tag="VEL68_15855"
     CDS             complement(3046..3750)
                     /locus_tag="VEL68_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37496.1"
                     /translation="MLEIQSINVYYGEFQALFSISLSVSEGETVITVGPNGAGKSTLL
                     KTISGLLHPRSGSITFMKNRIDRVPAHEIVELGITHVPEGGRLFPQLSVYENLRVGSY
                     LKRARGEAAATLKEIYRLFPILDERKKQLADTLSGGERQMLAIARCLMSRPKLILLDE
                     PSSGLAPRVVSRVLDFVHQIKSQGYAILMVEQNVRKALKLSDRAYLMESGRVHKEGTK
                     QSFQDDPYIKKAYIGL"
     gene            complement(3743..4483)
                     /locus_tag="VEL68_15860"
     CDS             complement(3743..4483)
                     /locus_tag="VEL68_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37497.1"
                     /translation="MLELIQVDRFFGHFQALKQINLKVEEGEFRGLIGPNGSGKTTIF
                     NVISGVYRASAGQVKFMGREVTEFTPDMICHAGITRSFQIPRPFREMTVVENVMIGSL
                     FGKGKENIVKGDPRAEAVHWLEFVGLKVDERTMPGELTAGNLRRLELARALATHPKLL
                     LADEIMSGLNQEEIILASQVLKKIREELKITIIWVEHIMNALMNLVERVTVLDYGQII
                     AEGTPEGISKNPKVIEAYLGEEETETHA"
     gene            complement(4580..5875)
                     /locus_tag="VEL68_15865"
     CDS             complement(4580..5875)
                     /locus_tag="VEL68_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37498.1"
                     /translation="MKKIFIVFLILSLCAGLQSTSWAKEKVKIAVMHSITGSLALAGG
                     LAGQRGSLVAIDMWNARGGILNQYDIVPVEADAQSNPDVAIREAERLINMEKVPVILG
                     IYSSAIATPLAPICERNKTILWITIAISDAVVEGKNLTYTFRPQVMGSQYGISAVEYL
                     KDNVAKLGYKDPSEIKIAITYEDGPYGVSCKTGGLNQAQKYKIKVVLTEGYDHKTKDL
                     SSLVLKLKAAKPDAVLNTGYYPDIVLFMRQAREMGFQTKALIGHGAGYGNFPNLEKQL
                     GPEMIQYVYNIDPAPAQSLNRSQLQKEMGPIIDEFLKRYNEKYKEASPPSHATMGFTH
                     AWVLLQTMELAVKKYGAPTADNIQKAALQIDIPEGGTTSGYGVKFNPPGHPFSGQNFR
                     CYPVVMQWVKGKVETVWPKAHQTIEPLLPAPKDWPLAVK"
     gene            complement(6054..6353)
                     /locus_tag="VEL68_15870"
     CDS             complement(6054..6353)
                     /locus_tag="VEL68_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37499.1"
                     /translation="MGSSSKEPLERDEPSAKALGELMDLFLKLNSKLREKISPRPKEE
                     KGKTEKTEMIIIILNSSSENTPRPSRQEKPKLSKPNWIDELCKDIEGEDGKEKSS"
     gene            complement(6373..7809)
                     /locus_tag="VEL68_15875"
     CDS             complement(6373..7809)
                     /locus_tag="VEL68_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HXZ37500.1"
                     /translation="MAKVLRNRRPRTVGELKKANIPVYSVKTEMRKNLIERIRKDQDF
                     FPGIIGYEETVIPQLENAILSGQDVIFLGERGQAKTRMIRSLIYLLDEEIPVIAGCEI
                     NDNPFYPICKSCTEKVQTLQDKVEIAWLGRDQRYAEKLATPDVTIADLIGEVDPIKVA
                     EGRYLSDELTIHYGLIPRTNRGIFAINELPDLGEKIQVGLFNIMEERDVQVRGYKIRL
                     PLDVYIVASANPEDYTSRGRIITPLKDRYGSEIRTHYPRTLKDEIEIMDQERARFDDD
                     GAFATTIPLFFKELIAEITALARKSPEINQRSGVSVRVSISNYENVISNALRRAIRVG
                     EGEVVPRVSDLPYLAASTSGKIEFETVEEDRNQTLIEKLIDKAVLNTFNRYFQVQQFD
                     AFLSRFKSGWSLEVSERMKSSEYQLKAQETSPHMAEMRGLLEGLKKLDCDQSPAQVAS
                     GLEFILEGLYLHHRLNKEKLPGKAAYRA"
     gene            8154..8750
                     /locus_tag="VEL68_15880"
     CDS             8154..8750
                     /locus_tag="VEL68_15880"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ37501.1"
                     /translation="MVKDVKEELKDLNLGQKIKNLRQKKGLTLQQVAENANLSEPVLV
                     QIEGEVVAPPVATLLKIARAMNVNIGYFFQDQDAEKRAIVVPKSDRKRVFRRIHEDPS
                     KVGYYYESLAYPKADKHMEPFHVTFEVKGKEDLIFLTHRGEEFIYVLDGQLEFTYENE
                     IYSLGAGDSLYFDSSLPHAFRAVGKKHATAIDVIYASE"
     gene            9147..11045
                     /locus_tag="VEL68_15885"
     CDS             9147..11045
                     /locus_tag="VEL68_15885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ37502.1"
                     /translation="MTKDFSDEVCAEMKKVYPEKFVSEENIFSRIHRGDRIFIHTACA
                     EPQYLVQALIKFVESHPKAFFDAEVVHVWTLGVAPYTDEKFKYNFRHNSFFVGDNSRE
                     AVNRGMADYTPLFLSDVPELFGRGMVPIQVALIQTSPPDEHGNMSLGISVDISKAAVE
                     KASVVIAQVNRHMPRVHGDGFIHIKDVNYILCHDEPLLEYEAIAPDVVAQQIGKYVAR
                     IVRDGDTIQVGYGALPNAILANLGQKNNLGVHSELLSDGMVELMKKGVIDNRRKSLNR
                     GKTVATFCLGHKSTYEYLQDNPAFEFRTIDYTNSVLVIARQENMTAINSALEIDLTGQ
                     TSSESLGKTFYSGPGGHTNFMRGATLAPGGRTILAIQSTAEWGKVSRIVPFLREGTGV
                     TLVRGDVHYVITEYGIAYLHGKNIRERAMELISIAHPQFRPWLIEKAKQANLIYRDQA
                     FIPGKRGEYPEHLESYRTTIKGLEVLMRPVRLSDEPLLKDFFYSLSDQSLYRRFISMR
                     KDMPHERLQEFVVIDYTKETVILATVREGEMEKVVGLGQYGINEANHTAEVALVVRDD
                     YQGKGVGTEVLSYLTFLAKREGLYGFTAEVLVENKPMLHLFEKMGFDIQKRSSQGVYE
                     LQMGFRGA"
     gene            11045..13174
                     /locus_tag="VEL68_15890"
     CDS             11045..13174
                     /locus_tag="VEL68_15890"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXZ37503.1"
                     /translation="MESPNLDIQNLFEPKTLAVIGAARDENKIGYKILKNILTGGYKG
                     KIFPVNPQGGEILGLKAYRSVEEVNETVDVASIVIPAKFVYEAVQSCARKKVKYLTII
                     SSGFSEIGNNEEERRIVSYARAHNMRVLGPNIFGVYSAAASLNATFGSSGISPGRVAI
                     ITQSGALGIAMIGKTAVENIGLSAIVSVGNKTDVDEADLLEYLVTDPHTKIVLMYIEG
                     VRDGERLIQAVKKTTRKIPVVVIKSGRSERGAIAAASHTGSLAGSDEIFDAIMRQCGV
                     LRAENVEEAFNWCKFLANTPSPAGESTVIVTNGGGIGVMATDACEKFGVRLYDDTLTL
                     KEIFSTVTPEFGSTKNPVDLTGGATSTHYNSALEEALKSAQIDSVIALYGETAIFDAE
                     NLSSMIVENTQRYKEKKKPLVFSIFGGEKIESCLLSMKKGEAAVYGDVYQAVSCLGSI
                     YRYYHYLREYSEAVDEAAIDPPTINRVIDQALKEGRSFLLAHEGQAVMRAAGIPIPQT
                     RIARSLEEAVRAAEAIRFPVVMKVVSRDILHKSDAGGVALDLEDKEEVIDAYQAILRN
                     VRAYKADANIEGVEVSEMVEKGMETIIGARRDRTFGPLIMFGLGGIYVEVMKDVAFRA
                     LPINRKEIMAMVKEIRAYPLLLGVRGEEKKDLDSVVDTIIKVGTVLQRCERITDIEIN
                     PLMAYEQGRGVKAVDVRILLTSGRKGV"
     gene            13175..14215
                     /locus_tag="VEL68_15895"
     CDS             13175..14215
                     /locus_tag="VEL68_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HXZ37504.1"
                     /translation="MEKVIIASMRRSAGKTSAIVGITAAAKKKIAYVKPFGERMLYRK
                     KRLWDYDSSLISALFGLKEDPVDMSIGFDHSKLRYMYDEEGTRKKLMETIANLANDRD
                     VLFIEGGRDLSYGISVHLDTLSLAKYIGGRLYIVVSGDDDSILDDLLFLKQHVNLSGV
                     NLNGVIINKVQNLEEFQSTHLPAIRQMGHQVIGVIPYQSELTHFTVSYLSERLFAKVL
                     CGEGGLTRVVKNILIGAWSANVFLQNPIFKKENKLVITGGDRTDMILASLESDTSGII
                     LTNNILPPSNIISKASERNIPLLMVFSDTYQTAKQIESLEPLLTKDDGEKVEMLKALF
                     QKHVNVQEILGK"
BASE COUNT         3318 a         3892 c         4228 g         3039 t
ORIGIN      
        1 tacatgcggg cccgggcgta tgtcagcgag gaattgagaa aaatgggcgc gcgggtctcg
       61 accaccgtgg ggggaaacct ttgcggccgc ctcgaaggga gcatccgagg aattccctcg
      121 gtgatgacgg gctctcacat tgacagcgtc cttcgagggg ggcggtttga cggtgcggca
      181 ggagtggtgg ccgccttgga ggtggccagg gtgatggcgg aggaaaagac cccccaccgg
      241 catcccatcg atgtggtcgt ctttgcggag gaagaaggat cacggtttgg ctcggtgatg
      301 atcggcagca gggcctggat cgggaaactc tcttcggagg acctgaatcg gtttaaggat
      361 gcggacggga ccagctactt tgcagccatg gaaaaggccg gcctcaaccc cggggatggc
      421 cctttgctga ggtccggcat ggtaaaaggc atgatcgaac tccatatcga gcagagcctt
      481 gtgcttgaga ataaggggtt cccgattggt gtggtggagg ggatcaacgg gatcaagcag
      541 tttctggtta ctataaccgg tgttcccaac cacgcgggcg caacccccat gggtcttcgg
      601 cacgacgctc ttcaaggggc agtcagggcg attgccgctg tagaagagat tgccatccag
      661 gacctggggg caaataccgt agccacggtc ggtatgttga cctgcgatcc gggccaggcc
      721 aatgtcatcc ccggcagagt ccagttcacg gtggacgttc gcgacctcga tagagaacgg
      781 atcgaccagg ccgcccagcg catcctatcc gtgattcaaa cgatttgccg ggccagaggc
      841 ctgaccttcg atatcaaacc ccggtcggat acccctccgg ttcgcctctc aaaggaactg
      901 gtccagctga tcgaagaaac ggctcaacag aagcaggtcc aggccttaag gatggcgagc
      961 ggcgcccttc atgattcttc catcctcccc gaagtgaccg aagtagggat gattttcgtg
     1021 ccgagcaagg ccggaagaag ccactgcccc gaggaggaga cggacctaag agacctccgg
     1081 acgggggccg atctcctcct gggcgcggtg gccaaactcg caacgtaacg acctgcctcg
     1141 gttttgtcgc ttattcaggt agcgaaggga ttgggccttc aaggagtaag cagaaacaag
     1201 ttctaggctc ttttcttctt gaacagctcc tgaagtgttc cgtagattcc cttgggcagg
     1261 aagatgatca gcaggacgag cgccagcccg tagatgatct gcgagaatcc cacgaaggtg
     1321 ctcccgtagg ccacccggaa atactcctcc agcgagacaa taagggccgt gcccaccgca
     1381 gggccccata gactgaacat tcctcccagg atcgccttga aggcgatttc gagcgaaggc
     1441 gggactcccg agagggtatg gggattcagg taagtgatgt actgtgcgta gagaaccgct
     1501 ccaatggcgg tcatgaaggc gctgatggag gtgatgatca tcttgtagcg gatgatcccc
     1561 acccccagag tggaagcggc aatttcgtcc tcgccgatgg cccggagggc tttttgcatc
     1621 ttggatcggt cgatccattt ccagaccagg agggcgatga tcaaaaaagc gacggaaatg
     1681 tagtagaaat gaatcttctg ctcggattga aaatataaga gagaggttcc tttggatttc
     1741 agcgtaaacc ccagtgaccc gccggtgata tcccggaagg cgatgatcgt gagggccacg
     1801 acctccccca gggccagagt gaccagggca aaatagtcgc cgatcactcc gaagcggaag
     1861 caggcgtagc cgatgagcat ggcaaagagg accgcgacca cccccccaag aagcattccg
     1921 atccagggag agagcccgta aaaattaaag agcaggcccg aagtataagc tccgaggcct
     1981 agaaaggcac catgccccag ggataccagc ccgaaccttc ccatgtacga ccaagcggtg
     2041 gtgatgaaag accagatgaa gatcaaggtt ccgatgtgga tcaagtaggg ggacggaaaa
     2101 aagatgggaa agacgccgat ggcggcgagg agaatgatca aaatcaaagg ggtgcgcaag
     2161 aagtccctcc tacttggcca ctcgaattcc cagaaggccc tgaggccgga ccaaaatcac
     2221 tgcgatgaag atgaccaggg caacgatgtc cgcgatctcc gtggaggtca tggcggcgga
     2281 tacggaagtg acgatgccga tgataaaggc gccgatgaac cctcccagga ggtttcccat
     2341 tccccccaac acgacgatga tgaaggccat catggtgaaa ctcccgccga aatgagggtg
     2401 gacggcataa atgggggaga agaaaccggc gatcagggcc gtaagcaatc ccccggccgc
     2461 catcgtgaga taatagatcg cctgggggtt gattcccatg agcctggcgc tgtccaggtc
     2521 ctgggcgatg gatcgaatgg ccagtcccat ataggtgcga ttcaggaaca agtacaggcc
     2581 ccacagggtc agcagggctc cgaagaaggc gatgagattg gaggtccgga gaaaaacatt
     2641 tccgatgctg atgatgggga gttgaacatt gatccccctc aggtccgccc cccagatcac
     2701 ctgggccagg ttctcaagca cgatggtcaa acctgccagg gcgaggagct gggtgagggg
     2761 attggccttg gcccggagca ggggattgat caccaggacc tgcaggccaa aaccgactac
     2821 tcccatgagg ggaagggtgg cgatgcaggc caggagaatc gggaacccca gatgggtgac
     2881 cagggtgtag ataaagtaga gcccgagcat gacgcactgg ccgtaggcca tgtggatgat
     2941 cttgaccacg ccgaagatca ggttcatccc gaaggccaac aaggccaaga ccccgccgag
     3001 caagatcccg ttgataaccg cattgagata aaggtcaagc atgaatcaca atccgatgta
     3061 agccttttta atgtagggat cgtcctggaa agactgtttc gtcccttctt tgtgcacccg
     3121 gccagactcc atgaggtagg cccggtcgga aagcttgagg gctttgcgca cgttctgctc
     3181 caccatcagt atggcatacc cttgagattt gatctggtgg acgaaatcca gaactcgaga
     3241 taccacccgg ggggccaggc ccgaagaggg ctcgtcgaga aggatcagct tgggccggga
     3301 catgaggcag cgggcgatgg ccagcatctg ccgttccccg ccgctcaggg tgtcggccag
     3361 ctgtttcttc ctttcgtcca ggatggggaa gaggcggtag atctccttca gggtcgccgc
     3421 tgcctccccc ctcgctcttt tcaagtagga tcccaccctg aggttttcgt aaacgctcag
     3481 ctgcggaaag agccgccctc cttccggcac atgggtgatt cccagttcga cgatctcgtg
     3541 agcggggact cggtcgatcc gatttttcat gaaggtgatg cttcccgagc gagggtggag
     3601 gaggccggag atggtcttga gaagggtcga tttccccgcc ccgttaggcc caacggtgat
     3661 caccgtttcc ccctcggaaa cgctaaggga gatgctgaag agcgcctgaa attcaccata
     3721 ataaacgttt atactttgaa tttcaagcat gggtttcggt ctcttcctct cccaggtagg
     3781 cctcgataac cttgggattc ttggagatcc cctccggggt cccctcggcg atgatctgtc
     3841 cgtagtccag gaccgtcacc cgctccacca ggttcatgag ggcgttcatg atatgctcca
     3901 cccagatgat ggtgatcttc aattcttccc ggattttttt caggacctgg gaggccagga
     3961 tgatctcttc ctggttgaga ccgctcatga tttcatcggc aagaaggagt ttggggtgag
     4021 tggccagagc ccgggccagt tccaggcggc gaaggttccc ggcggtcaat tcgccaggca
     4081 tagttcgctc atccactttg agacccacga actccagcca gtggacggct tcagcccggg
     4141 gatctccctt gacgatgttc tccttcccct ttccgaagag ggatccgatc atcacgtttt
     4201 ccaccacggt catctcccgg aaaggcctgg gaatctggaa ggaccgggtg attccggcgt
     4261 ggcaaatcat atcgggcgtg aattcagtga cctctcggcc catgaatttc acctgcccgg
     4321 ctgaggcccg gtaaactccg gagatgacat tgaagatggt cgtctttccg gacccgttag
     4381 ggccgatgag gccgcggaac tccccctctt caaccttgag attgatctgc ttcagggcct
     4441 ggaagtggcc gaagaatcgg tccacctgga tgagctctaa catggcgccg gcagaaagga
     4501 ccttcctggg ttcatacgca agtaggggcg gatttcaacc ccgcccctac ggtttaaaaa
     4561 cactctatcc ttaaagattt tatttgaccg ccaggggcca gtctttaggg gccggcaaca
     4621 gcggttcgat ggtctggtgt gccttgggcc aaaccgtttc caccttaccc ttgacccatt
     4681 gcatcacgac cgggtagcaa cgaaagttct ggccggagaa tgggtggcca gggggattaa
     4741 acttgacccc gtaacccgag gtagttcctc cttccgggat gtcgatttgc agcgccgctt
     4801 tttggatgtt atcggcagtg ggggcgccgt attttttaac ggccagttcc atagtttgga
     4861 gaaggaccca ggcatgggtg aagcccatgg tggcgtgcga cgggggggag gcttccttgt
     4921 atttttcgtt ataacgcttc aggaattcgt cgatgatcgg ccccatttct ttctgcaatt
     4981 ggctcctatt caaactctgg gccggagcag gatcgatgtt atacacgtac tgaatcattt
     5041 ccggccccaa ttgtttttcc aggttcggga aattgccgta cccggcgcca tgaccgatga
     5101 gggccttggt ctgaaagccc atttcccttg cctggcgcat aaagaggacg atatccggat
     5161 aatacccggt gttcaagacc gcatccggtt tggccgcctt cagtttcagg acgagagaag
     5221 aaaggtcctt ggttttgtgg tcgtagcctt cggtcaggac cacttttatt ttatatttct
     5281 gggcctgatt gaggcctccg gttttacagc ttaccccgta gggcccgtct tcatacgtga
     5341 tggcgatctt gatctcgctg gggtccttgt agccgagctt ggccacgttg tccttgagat
     5401 actccacggc gctgatcccg tattgggacc ccataacctg cggacggaag gtataggtga
     5461 ggttcttgcc ttccaccacc gcgtcggaaa tggcgatggt gatccagagg atcgtcttgt
     5521 ttcgctcgca gatcggcgcg aggggggtgg caatagcgct ggaatagatc cccagaatca
     5581 ccggaacttt ttccatgttg atcagccgct ccgcctcgcg gatggccacg tcagggttgg
     5641 actgggcgtc agcctccacc gggacgatgt cgtattggtt aaggattcct ccccgggcgt
     5701 tccacatgtc gatggccacg agggagcctc tctgcccggc caaacctccg gccagtgcga
     5761 gggacccggt gatggagtgc atgaccgcga ttttgacctt ttcttttgcc caagacgtgg
     5821 attggagacc ggcgcacaac gacaggatta aaaagacgat gaagattttt ttcatctctc
     5881 ctcctccttt ttggcgaatg aaattcggct attgatggac cactcgaatc ggcgatgatg
     5941 atattagtca atggagaggg ggctgtcaag gaatttttcc cggaaagagg agaatgaaca
     6001 gcttcccttt ccaatggaag gcggaagaaa acgagtccgc aaaggcgatc cctctaagaa
     6061 gacttttcct tgccgtcttc cccttcgata tctttgcaga gttcgtcgat ccagttgggc
     6121 ttggaaagct tgggtttttc ctggcgggag ggccgggggg tattttcaga agaactgttc
     6181 aggatgatga tgatcatctc cgttttttcc gttttccctt tttcttcctt ggggcggggg
     6241 gaaatctttt cccggagttt ggaattgagt ttcaggaaga ggtccatcag ttcgccgagg
     6301 gccttggctg aaggttcgtc tctttcaagc ggctccttac tggaagatcc catggttatt
     6361 tttctttccc ctttaggcgc ggtaagcggc tttaccgggc aatttctctt tgttcaggcg
     6421 gtggtggagg taaagccctt ccaggataaa ctccagcccc gatgccacct gggccggact
     6481 ctgatcgcaa tccaattttt tcaaaccctc cagcagcccc ctcatctcgg ccatgtgggg
     6541 ggatgtctcc tgggctttga gctggtattc ggaagatttc atccgctcgg aaacttccag
     6601 ggaccatcct gatttgaacc gcgaaaggaa ggcgtcaaac tgctgtacct gaaaataccg
     6661 gttgaaagta ttgagcaccg ccttgtcgat cagtttttca atcaaggtct gattgcggtc
     6721 ttcctccact gtctcgaact cgattttccc gctggtggaa gccgccagat agggcaggtc
     6781 gctgacccgg ggaaccactt ccccttcccc tacccggatg gcccggcgca gggcgttgct
     6841 gattacgttt tcgtagttgg aaatagagac tcgaaccgac acgccggagc gctgattgat
     6901 ctctggggac ttgcgggcta gggcggtgat ttcggcgata agctctttga aaaaaagcgg
     6961 aatggtggtg gcgaaggccc cgtcatcatc aaaccgggcc cgttcctggt ccatgatctc
     7021 gatttcatcc ttcagcgtcc ggggatagtg agtccgaatt tccgagccgt accgatcctt
     7081 gaggggagtg atgattcggc cccggctggt gtagtcctcc gggttggcgc tggctacgat
     7141 gtaaacgtcc aggggaagac ggatcttgta cccgcgaacc tggacgtccc gctcttccat
     7201 gatgttaaaa agccccacct ggatcttctc ccccaggtcc ggaagctcgt tgatggcgaa
     7261 gatcccgcgg ttcgtccggg gtatgagtcc atagtggatg gtcagctcat cggagaggta
     7321 gcggccttcg gccaccttga tggggtctac ttccccgatg aggtcggcaa tggtcacgtc
     7381 cggagtggcc agcttctcgg cgtaccgctg atcccttccc agccaggcga tttccacctt
     7441 atcctggagg gtttggacct tctccgtgca gcttttgcag atggggtaaa aagggttatc
     7501 gttgatctcg cagccggcga tgaccggaat ttcctcatcc agaaggtaga tcaaactgcg
     7561 aatcatgcga gtcttcgcct gtcctcgctc acccagaaag atcacgtctt gtccggaaag
     7621 gatggcattt tccagctggg ggattaccgt ttcttcgtag ccgatgatcc ccgggaaaaa
     7681 atcctgatcc ttacggatcc tctcgatcag gttcttacgc atctccgtct tgacggaata
     7741 aaccggaatg ttcgcctttt tgagctctcc cacagtgcgg ggtcttctat ttctaaggac
     7801 tttagccacc accatttcct ttccttttct tggttccggg ttgcccgatc gttgtttctc
     7861 tttacttgat tccctgccaa ccacccacga gcagcccgta atgacttttt actatcttca
     7921 cttatagctc agaaaccccg ctccgtcaag gccgggccat aaaattcgga atgataaata
     7981 aggaggatac ggaaaagaga agaatcagcg gaacagaagt caaaaaccag caaaggagga
     8041 gtaaaatcta gaggaattaa atttttgttg aaaaaattac atgttcacgt tagatttgag
     8101 caacgcttga gcggaagcca ggaatatttc caattttttg atcggagggg tagatggtca
     8161 aggatgtgaa agaggaattg aaggacctga atctcggtca gaaaatcaaa aacttgagac
     8221 agaaaaaggg gctcacctta caacaagtag ccgagaacgc aaatctctcc gaaccggtgc
     8281 tggtgcagat cgagggggaa gtcgtggctc caccagtcgc gaccttgctg aaaatcgcca
     8341 gggccatgaa cgtgaacatc gggtattttt tccaggacca ggatgccgaa aagagggcca
     8401 tcgtcgtccc caagagcgat cgcaagaggg tcttccggcg cattcatgag gacccctcga
     8461 aggtgggtta ttactacgaa tccctggctt atcccaaggc ggacaaacac atggagcctt
     8521 ttcatgtgac ctttgaggtg aagggaaaag aagacctcat cttcctcacc cacagaggtg
     8581 aggagttcat ctatgttctg gacgggcagc tggaattcac ctatgagaat gaaatctact
     8641 ccctgggggc gggggatagc ctttattttg actccagcct cccccacgcc ttccgagctg
     8701 tggggaaaaa acacgccacc gccatcgacg taatctatgc ctccgaatag aagaaccccg
     8761 cccagcagag cgcagggatg aaggaaaagc attctgcagt tcggagaggg tcccgtgtcg
     8821 ggtcctccca cctgggggcc ctagcacggc aaaaccccag ccccgcataa cctccatttc
     8881 cccctttcct ctttaatacg gtcttccttg ccccctcact tccgggccgg gcattgcccg
     8941 aaattcaata gggctcgagc ccacttgccg aaaagggaag ggggaagttc ggtcttcccc
     9001 ttgacgggtg atcggttata agaatagaat tcagatgaaa tagaagaggc atagaaaaag
     9061 acgggaaagc cgctcgctgt agcaaaaact ggcgccggct ctctcccgat gaaggaaatg
     9121 aggagaagaa tcaggtgtct tccgcaatga ccaaggattt cagcgatgaa gtctgcgcag
     9181 aaatgaagaa ggtctacccg gagaagtttg tttccgagga gaacatcttc agccgcatcc
     9241 atcgagggga tcgaatcttc atccataccg cctgcgccga accccagtat ctggtccaag
     9301 cgctgattaa gttcgtggaa tcccatccca aagccttctt cgacgccgag gtcgtccacg
     9361 tctggaccct gggggtggcg ccctacaccg atgagaaatt caagtacaat ttccgccaca
     9421 actccttttt cgtgggggac aactcccggg aagcggtgaa ccggggcatg gccgactaca
     9481 cccctctctt tctttccgat gtgccggaat tgttcggccg gggaatggtc cccatccagg
     9541 tggccttgat ccagacttcc cccccggacg agcatggaaa catgagcctg ggaatcagcg
     9601 tggacataag caaagcggcg gttgagaaag cctccgtggt catcgcccag gtgaaccgcc
     9661 acatgccccg ggtgcacggg gatgggttta ttcacatcaa ggatgtgaat tatatcctct
     9721 gccatgatga accccttctg gagtacgaag ccatcgcccc ggatgttgtg gcccagcaga
     9781 tcggcaagta cgtggcccgc attgtccggg acggagacac gattcaggtg gggtacggcg
     9841 cgctgcccaa cgcaattctc gccaatctgg gccaaaagaa caacttgggg gtgcactccg
     9901 agctactgag cgacgggatg gtggaattga tgaagaaggg ggttatcgat aaccgccgca
     9961 aatcgctcaa ccggggcaag acggtggcca ccttttgcct gggacacaaa tccacctatg
    10021 agtacctcca ggacaatccg gccttcgagt ttcggaccat cgactacacc aacagcgtcc
    10081 tggtgatcgc ccgccaggaa aacatgaccg ccatcaacag cgcgctggag atcgacctga
    10141 ccgggcagac gtcctccgaa tccctgggaa agactttcta cagcggaccg ggagggcaca
    10201 ccaatttcat gagaggggcc accctggccc ccggcggaag aaccattctg gccattcaat
    10261 ccacggctga atgggggaag gtatcccgca tcgttccctt cctgcgcgag gggacgggcg
    10321 tgaccctggt ccggggcgat gtccactacg tgatcaccga atacgggatc gcctacctcc
    10381 acggcaagaa tatccgcgaa cgggccatgg agctgatctc catcgcccat ccccaattcc
    10441 gcccctggct gatcgaaaag gccaagcagg ccaacctgat ctaccgggat caggccttca
    10501 tccccggaaa aaggggggag taccccgagc atctggaaag ctaccgcacc acgatcaaag
    10561 gtctggaagt tctgatgcgg ccggtccggc tcagcgatga gccgctgctc aaggacttct
    10621 tctactcgct ttccgaccag agcctgtacc ggcgattcat ctccatgcgc aaggacatgc
    10681 cccacgagcg gctgcaggag ttcgtggtca tcgactacac caaggagacg gtcatcctgg
    10741 ccacggtgcg ggagggggaa atggaaaagg tggtggggtt gggccagtac gggatcaacg
    10801 aggccaacca taccgccgag gtggccttgg tggtgcggga cgattaccag ggtaagggtg
    10861 tggggacgga ggttctttcc tacctgacct ttctggccaa acgggaggga ttatacggtt
    10921 tcaccgccga agtcctggtg gagaacaagc ccatgctcca tctgtttgaa aagatggggt
    10981 tcgatatcca gaagagaagc tcgcagggag tgtacgaact gcagatgggt ttcagaggag
    11041 cctgatggaa agcccaaatt tagatattca aaacctgttt gaacccaaga ccctggccgt
    11101 catcggagcg gcgcgggatg aaaataaaat aggctacaag atcttgaaga atattctgac
    11161 cggcggatac aaggggaaaa tctttcccgt gaatccccag gggggcgaaa tcttgggcct
    11221 gaaggcctac cgctccgtcg aggaagtgaa cgaaacggtg gatgtggcca gcatcgtcat
    11281 ccccgccaag ttcgtctatg aagcggtcca gagctgcgcc aggaagaagg tcaagtacct
    11341 gaccatcatc tcctccggct tctcggagat cgggaacaac gaggaagagc gccggatcgt
    11401 gtcctacgcc cgtgcccaca acatgagggt cctggggccc aacatcttcg gggtctactc
    11461 ggccgcggcc tccttgaatg ccaccttcgg cagctcgggc atcagccccg ggagggtggc
    11521 cattatcacc cagagcgggg ccttgggcat cgccatgatc ggcaagaccg cggtggagaa
    11581 catcggcctt tcggccatcg tctccgtggg aaacaaaacc gatgtggacg aggccgacct
    11641 tctggaatac ctggtgaccg atccccatac gaagatcgtt ttgatgtaca tcgaaggggt
    11701 gcgggacggg gagcggctca tccaggcggt taagaagacc acccggaaaa ttcccgtggt
    11761 ggtcatcaag tccgggcggt cagaacgggg ggccatcgcc gccgcctccc ataccggttc
    11821 cctggccgga tcggacgaga tcttcgacgc catcatgagg caatgcgggg ttctgcgggc
    11881 agagaacgtg gaggaagcct tcaactggtg taagttcctg gccaataccc cttcccccgc
    11941 cggggaaagc acggtcatcg tcaccaatgg gggcggaatc ggggtaatgg ccaccgacgc
    12001 ctgcgagaag tttggggtga ggctgtacga tgataccctg accctcaagg aaattttctc
    12061 cacggtcacg ccggagttcg gttccaccaa gaacccggta gacctcaccg ggggagccac
    12121 ctccactcat tacaactcgg ccctggaaga ggccctgaag agcgcccaga tcgactcggt
    12181 gatcgccttg tacggagaaa ccgccatttt cgacgcggaa aacctttctt ccatgatcgt
    12241 ggaaaatacg cagcggtaca aagaaaagaa aaagcccctg gtcttttcca tctttggggg
    12301 ggagaagatc gaaagctgcc tgctttccat gaagaaaggg gaagcggccg tatacgggga
    12361 cgtttaccaa gcggtctcct gcctgggctc gatctaccgg tattatcatt atttaagaga
    12421 atattccgaa gcagtggatg aggcggccat tgacccgcca accattaacc gggtgatcga
    12481 ccaggccctg aaagagggaa ggtccttttt acttgcgcac gaaggacagg cggtcatgcg
    12541 cgccgccgga atccccattc ctcagacccg gattgcccgc agtctggaag aggcggttcg
    12601 ggccgcggaa gccattcgct ttccggtggt catgaaggtg gtttcccggg acatcctgca
    12661 taagagcgac gccggcggag tagccctgga cctggaggac aaggaggagg tcattgacgc
    12721 ctaccaggcc attttgcgca acgtgcgggc ctacaaggct gatgccaaca tcgagggggt
    12781 ggaggtctcc gagatggtgg agaaggggat ggagacgatc atcggggcca ggcgggaccg
    12841 gaccttcggc cccctgatca tgttcggcct gggcggcatc tacgtggaag tgatgaaaga
    12901 cgtggccttt cgggctttgc ccatcaaccg gaaagaaatc atggccatgg tcaaggagat
    12961 tcgggcttac ccgctcctcc tgggggtgcg gggagaggaa aaaaaggacc tggacagcgt
    13021 ggtggatacg atcatcaaag tagggaccgt tctgcagcga tgcgagcgca tcacggacat
    13081 cgagatcaat cccctcatgg cttacgaaca ggggcggggg gtcaaggccg tggatgtgcg
    13141 gattctgcta accagcggga ggaagggagt ataaatggaa aaagtaatta tcgcctcgat
    13201 gcgcaggagc gcgggcaaaa ccagcgccat tgtggggatc accgctgcgg cgaagaagaa
    13261 gatcgcctat gtcaaaccct tcggggagag gatgctctac cggaagaagc ggctttggga
    13321 ctacgattcc tcgctgatca gcgccctctt cggactgaaa gaggaccccg tggacatgag
    13381 tatcggtttt gaccactcca agcttcgcta catgtacgat gaagagggga cccggaagaa
    13441 gctgatggag accatcgcca acctggccaa cgaccgggac gtgctcttca tcgagggagg
    13501 gagggacctg agctacggaa tttcggtcca cctggacact ctctccttgg ccaagtacat
    13561 cgggggaagg ctctacattg tggtcagcgg ggacgacgac tcgatcctcg acgatctcct
    13621 cttcttgaaa caacatgtga atctttccgg ggtcaacctg aacggggtca tcataaacaa
    13681 ggtgcagaac ctcgaagagt ttcagagcac ccaccttccg gccatccgcc agatgggtca
    13741 tcaggtaatc ggggtgatcc cctatcagag tgaactcacc cacttcacag tatcctatct
    13801 gtccgagcgt ctcttcgcca aagtcctttg cggcgaaggg ggactcaccc gggtggtgaa
    13861 gaacattctg atcggggcct ggtcggccaa tgtatttctc cagaatccca tcttcaagaa
    13921 agagaacaag ctggtcatca ctggcgggga ccgcaccgat atgatcctgg cttccctgga
    13981 aagcgatacc tcgggaatca ttctgacgaa taatattctt ccaccctcga acatcatcag
    14041 caaggcttcc gagcggaaca ttcctctttt aatggtcttt tccgatacct accagaccgc
    14101 caagcagatt gagagcctgg aaccgctgct caccaaagac gacggggaga aggttgaaat
    14161 gctgaaggcg ctgttccaga agcatgtcaa cgtgcaggaa atactgggta aatagaaaat
    14221 gggtcatgcg gtcataggtc agggatgaca acaaatccct gatgggtcgc tgctacggcc
    14281 tcgtccctca aaccttaaac ctctaacctg cttttgatta tggccaaggt gattagaaga
    14341 agttccaaga aagcggggct tcctcccggg acgctggtcc atatcggaga gaagaaaacg
    14401 gagaagaccc gcgttgcagt ggtggaatac gacggccaga gctttcagga aaaagagctg
    14461 gagactctcg agacctg
//
LOCUS       DASPRR010000433        15858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1395250, whole genome shotgun sequence.
ACCESSION   DASPRR010000433 DASPRR010000000
VERSION     DASPRR010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15858)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15858
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395250"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VEL68_15900"
     CDS             <1..325
                     /locus_tag="VEL68_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXZ37505.1"
                     /translation="VEGFAMLKELAQGLGGAVGATRVPVDEKWVPLSQEIGQTGKIVS
                     PELYIAVGISGAAQHITGILNSKCIVAINKDPDANIFKVANYGVAADFRQVLPTLIEK
                     IKSAR"
     gene            complement(468..1502)
                     /locus_tag="VEL68_15905"
     CDS             complement(468..1502)
                     /locus_tag="VEL68_15905"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HXZ37506.1"
                     /translation="MDLTEVRETAKEKMKGFCGVYKVCDGLPERLCQGIKYGKAIGMG
                     GIGKGESFAANVKALDRLRLKTRLITPHVEPNMRASFVGKEVAFPIFCTSMSGVKISM
                     GGGITELEFARAAIHGSKAAGTIGFIGDGAETFEDRPGLQAIKEAAGWGVQIFKPREQ
                     KVLFRLIQEAEKAGAIAVGVDVDGAGSVPMSMKGQPVFRKSVAELKELVRSTSLPFIF
                     KGITSEEDAYAALETGCKVVSISNHGGRTLDSMLGIAEVLPRLVPILKGKVTLTADGG
                     VRTGFDVIKMLALGADFVMVGREMIRAAIGGGVDGVRTEMEFLAKDLHKAMIMTSCNS
                     ISDINERIFA"
     gene            1857..3809
                     /locus_tag="VEL68_15910"
     CDS             1857..3809
                     /locus_tag="VEL68_15910"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXZ37507.1"
                     /translation="MAKNLTHKILEAHLVQGELIRGKEIAIRIDHTLLQDATGTMAML
                     EFEALGMERVKVELAAQYVDHNILQTDNRNADDHKYLQTACARYGIRFSPPGNGVSHQ
                     VHMERFGVPGKTLLGADSHTTGAAGVSMLGIGAGGLDVALAMAGYPYFLPCPIVVGVK
                     LKGELPDWVSAKDVILEMLRRFGVKRCVGKIVEYYGPGVKSLSATDRETIGNMGAELG
                     ATSSIFPSDENTRAYLEAQGRGNAWKELAPDEGAQYDEHEEIDLRALEPLIARPTSPG
                     NVVAVREVAGIKVDQVIVGSSVNSSFRDLMVVAKIMEGRHCHPETSLNINPGSRQVLE
                     NIVSQSGAMSLMHAGARIYEAGCLGCIGMGQAPGTGQVSLRTFPRNFPGRSGTKEDQV
                     YLCSPETAAAAALKGVITDPRELAKEMAYPRIQGPARYIIDESSVIFPSDDLRETIVL
                     RGPNIKPLPQLDPLPERLEAEVVLKVGDNISTDTIMPAGNKILPLRSNIEAISEFIFS
                     PIDPNFAKECREKGGGVVIGGENYGQGSSREHAALGPRYLGVRAKIVKSFARIHKANL
                     CNFGILPLTFKDLRDYELLQKGSKVLFPDLRRRVEKGDREIPLQVDGRTMISLLEVSD
                     RQRRHLLAGGTLNSVRKELQGGKPKR"
     gene            3849..5030
                     /locus_tag="VEL68_15915"
     CDS             3849..5030
                     /locus_tag="VEL68_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXZ37508.1"
                     /translation="MAEAVRVKNIGLRGVTVADTKISFIDGEQGILIYRGYRIEDLAR
                     NSSYLETAYLLLNGNLPDLQQLETFTRQVVEAREIPKFIYDSLKKWPRDAHPMDVLQA
                     SIPILAMADPDIHQETREAAVRQAIRLISRLPGVVAGWHRIRKGQRPLPANDRLSHAA
                     NFLRQLTGKKPDGEVAHDLDVCLVLHADHTFNASTFACREVVSTRAHMYAAVAAGMGA
                     LSGSLHGGANTQVMKMLQELSGEKDIAAWVKKQLDQGEKIMGMGHAVYKTTDPRANFL
                     KDMGLRLGKKLGQERLPEISRELEKAAVEEFEKRGNPTIKPNVDFFSAPVYHMMGIPA
                     DMMTPVFAIARIAGWCAHIIEEAFAEAQEKPALYRPKSEYVGNYCGLMGCSYEPLQAR
                     K"
     gene            complement(5073..6242)
                     /gene="sucC"
                     /locus_tag="VEL68_15920"
     CDS             complement(5073..6242)
                     /gene="sucC"
                     /locus_tag="VEL68_15920"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXZ37509.1"
                     /translation="MNIHEYQAKEILFRYGVKVPQGHVSFDLRDAEAAAQGIGMGPFV
                     VKAQVHAGGRGKAGGIRKAKDPAEVREIARSMFGTRLVTAQSGPQGKPVSRVLVEEAL
                     PIEKEFYLGVTIDRKQGKVVVIGCSEGGMEIEEVSRRTPEKVHKLAVESGCGFTNFQG
                     RRLALDMGLQGPAIGKGAAVTSALVRAFMDCDCSLTEINPLALTVRGDLVALDAKMNI
                     DDNALYRHPELMALRDIEQEDPRDVEASRLNLNYINLTGNIGCIVNGAGLGMATLDLL
                     KYYGGEAANFLDAGAGATKDMIRNAFGLLRSDPKVRGIFINMFGGITRCDSYAQGVVD
                     ALRERPLTIPLVVRMEGTNVELGRKILEDSNLNILAYKSMREAAQKIIELVKEKK"
     gene            complement(6335..7033)
                     /locus_tag="VEL68_15925"
     CDS             complement(6335..7033)
                     /locus_tag="VEL68_15925"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HXZ37510.1"
                     /translation="MMQIFKIYRFNPEEDKAPYYKTYEVDIQEGMTILDTLNEIKWKQ
                     DGSLTFRRSCRHGICGSCAMTINGLNKLACETQVKSLGGGTVEIEPLRHFKVIKDLAV
                     DMEDFFQKLEEVKPYLIAGTPPTDQERYQSIEDRKKIDGSYECILCGACTSSCPSYWA
                     NPNFLGPAALLKAYRYVFDSRDEGFAERVPVLNTRHGLWRCHTIFNCTICPKSLNPTW
                     AIGELKKKLVGERY"
     gene            complement(7186..8916)
                     /gene="sdhA"
                     /locus_tag="VEL68_15930"
     CDS             complement(7186..8916)
                     /gene="sdhA"
                     /locus_tag="VEL68_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HXZ37511.1"
                     /translation="MVHEHDVVIVGAGIAGLYAALESSKHLNTAVISKVYPTRSHSIS
                     AQGGTAAALGSLEEDHWEWHLYDTVRGADYLGDQDAQEVLVRDAIEVAYELEHMGCPF
                     SRTDQGKVAQREFGGHYSNFGKGGAIRRMCYAADRTGHAMLHTLYEQCIKHDVRFYSE
                     FFVVSLLMEKNVCSGVVAWDMRNGGLQVFHAKTVMFGTGGYARAWKVNTNALSNTGDG
                     MSLVLQAGLPLEDMEFVQFHPTGLYPSGVLVTEGARGEGGYLVNHEGKRFMEKYAKAK
                     MELAPRDVVSRSIQQEIDEGRGIDGKCYVHLDLRHLGREAIMEKLPQIHELILKFSGI
                     DAVTGFVPIQPTAHYAMGGIPTNVDGQVIMDAKNTPVTGFYAAGECACISVHGANRLG
                     CNSTMDCAVFGRRTGRTMVKYILGGAEKPDLPKDGLEKAKAKIDWYLSLNGKDSPAAI
                     RDELQQSMMLNCWIFREEKLLKKQLEIIKSLQERFKNISIGYKGKRFNTELLDAIELG
                     NILDFVETIVVGGQARTESRGAHFRRDFPKRDDGNWLKHTLAWKTDTGIRLDYKPVVI
                     TKYKPEERKF"
     gene            complement(9079..9408)
                     /locus_tag="VEL68_15935"
     CDS             complement(9079..9408)
                     /locus_tag="VEL68_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37512.1"
                     /translation="MALPASHQSGAFHWLFQRITGILIAVVLIIHLGAVHFGASPVDL
                     GSPFWKFFHVGFLIMLLYHILNGFWLMVEDYVQCNGLRVTLFGVAWILGVSFLILGFV
                     TLVPFGT"
     gene            complement(9421..9744)
                     /gene="sdhC"
                     /locus_tag="VEL68_15940"
     CDS             complement(9421..9744)
                     /gene="sdhC"
                     /locus_tag="VEL68_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HXZ37513.1"
                     /translation="MRYKWQTGATAWLIHRITGAAVGIYLLLHILAQFGMKYDQKGLA
                     GISNFLTHKVVVVILLAFLIYHALNGIRELIIDFGSGALYHKKLFWAMMVIGLVFFVA
                     GLRAI"
     gene            complement(9996..10550)
                     /locus_tag="VEL68_15945"
     CDS             complement(9996..10550)
                     /locus_tag="VEL68_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HXZ37514.1"
                     /translation="MSQAIRLKTPLSDADVEKLKAGDKVLITGVIYTGRDAAHKRLFD
                     LLKEGKPLPIDLKGQIIYYVGPAPTKPGHVYGSAGPTTSGRMDAYSPKLMEIGLKGMI
                     GKGMRKKEVIEAMKKYKAIYFAATGGAGALLAKSVKKAEIVAYEDLGPEAISRLEVVD
                     FPAIVVNDTKGNDLYQQGMAQYAR"
     gene            complement(10564..11403)
                     /locus_tag="VEL68_15950"
     CDS             complement(10564..11403)
                     /locus_tag="VEL68_15950"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXZ37515.1"
                     /translation="MREVNAKQITDVVKKMCIDSNYYLTEDVLKALDEGEKKEESPVG
                     KEVFQQIKENVRIAREEQVAMCQDTGLAVLFIDLGQEVHIVGGSFSEAIHEGVRQGYK
                     EGYLRKSSCHPFTRVNTKDNTPAIIHLNLVPGDKIKIVLAPKGGGAENMSTVIMLSPS
                     EGIEGVKNRVVEWVKQSGSNPCPPVVVGVGVGGTFEEVALTAKKALLRPLGQKNPDPQ
                     LAAMEAELLTRINNLGIGPQGLGGRITALAVNVELIPCHIASFPMAVNLNCHAHRHQE
                     AVI"
     gene            complement(11500..12834)
                     /locus_tag="VEL68_15955"
     CDS             complement(11500..12834)
                     /locus_tag="VEL68_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXZ37516.1"
                     /translation="MLTKEQLLEKSKKPSADALKMHPYYKGKIASAVKCCVRDFNDFA
                     IWYTPGVAAACRDIEAHPEKVFEHTNKGNMVAVVSDGTRVLGLGDIGPEAAIPVMEGK
                     SILFKYLGGVDAWPVCLDTKDAEEIIKTVKLISPCFGGINLEDLSQPKCFHILDTLRK
                     ESKIPVWHDDQQGTAAVTLAGLINAFKVVKKNIKKARITLIGSGAANIACARVMLSYG
                     VDPAKLIMVDSKGTLHKGRTDIPPDYAEKRRFSQITNAEQRKGNIAEAMEGADAVVAL
                     AKSGPDVIKKEWVKKMAKDSILFACANPIPEIYPWEAEEAGAKVIATGRSDFPNQVNN
                     SLCFPAIFRGTLDIMATTITDEMCIASAVELAKCAEDKGLKKDYLLPLMSEREVFPRQ
                     AVAVGLKGIEQKVARVKMSKEQLFEKASAMIDRARREVELMMNSGIIPECKI"
     gene            complement(12923..13324)
                     /gene="mce"
                     /locus_tag="VEL68_15960"
     CDS             complement(12923..13324)
                     /gene="mce"
                     /locus_tag="VEL68_15960"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXZ37517.1"
                     /translation="MKILKIDHIGIAAKSIEQVAPFWNVILGLPIEGRETVQEQKATT
                     AFLPVGESELEILESTAPDGAIAKFIESRGEGIQHIALRVDNIEEALKELKEKGVRLI
                     DEKPRRGAGGAKIAFIHPKSTNGVLLELSER"
     gene            complement(13375..15051)
                     /locus_tag="VEL68_15965"
     CDS             complement(13375..15051)
                     /locus_tag="VEL68_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXZ37518.1"
                     /translation="MFDPKKSEEIGKQAKEWNGKIEKTLAKNPERRKTFQTTSGIPVK
                     RIFTPEDVAGLDYEQDLNFPGEFPYTRGVQPTMYRGRFWTMRQYAGFGTAEDTNRRYR
                     YLLEQGQTGLSVAFDLPTQIGYDSDHPLAQGEVGKVGVAIDSVKDMEILFDQIPLDKV
                     STSMTINAPASILLAMYIAVGEKQGVAAAKLNGTIQNDILKEYSSRGTYIFPPRPSMR
                     IITNTFEYCTKEVPNWNTISISGYHMREAGCTAVQEVAFTLANGVSYVQAAIKAGLDV
                     DEFGPRISFFFNAHLDFLEEVAKYRAARRLWAKIMRDRFKAQNPRSWMIRFHTQTAGS
                     TLTAQQPYNNIIRVAWQALAAVLGGTQSLHTNSMDEALALPSEQSVQVALRTQQLIAH
                     ESGSADTIDPLGGSYYIEKLTDEIEKRAMDYIDKIDQLGGSVVAVEKGYIQQEIQDAA
                     YRYQKEIESGDRVIVGVNRFQSKEPPPKGLLKVDPKVREVQIKRIAELKTSRSSQGVK
                     TSLEALKKVARGEGNLMVPILSCVRTHCTLGEVCDALRSVFGEYESAVKV"
     gene            complement(15158..15799)
                     /locus_tag="VEL68_15970"
     CDS             complement(15158..15799)
                     /locus_tag="VEL68_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXZ37519.1"
                     /translation="MQVQARVKDQSLISGKRSQILEGAIQVFKKKGYHKATVREIARE
                     AGIGLGSIYDYVNSKDDILYLFFENYANTFFEKVRSRASMVADPLQRLEITYRAFLEV
                     AMELEDQVMLSYTQARYVQKDYLKIILKKESEIVEHFEKIIGELGPGPFDPFLEANFL
                     VYSGVFGVLRRWILKPRYSREEIIDYLLQSQVQEVIRRIRGKTVRGSEFGVRS"
BASE COUNT         3233 a         4624 c         4379 g         3622 t
ORIGIN      
        1 cgtcgaaggg ttcgccatgc tcaaggaact tgcccaggga ctgggcgggg cggtgggagc
       61 cactcgggtc ccggtcgatg aaaagtgggt tcccctcagt caggagatcg ggcagacggg
      121 aaagatcgtt agccccgaac tctacatcgc cgtggggatc tcgggggccg cccagcacat
      181 cactggcatt ctcaactcca aatgcatcgt ggccattaac aaggacccgg atgcgaatat
      241 cttcaaggtg gccaactacg gcgtagcggc cgatttccgg caagtccttc ccaccctgat
      301 cgaaaaaatt aaaagcgccc gttgaaggaa gaagcaagtc cctcccctct cgggggaggg
      361 actgaccttt aaccgaatcg cgaacaacgg acctctttgg cctcaaagta gggtgttccg
      421 gtggcccttc aaaaaaagcc gcaagcggct tacaggctcc gaaaaaccta ggcaaaaatc
      481 cgttcgttga tatccgagat ggaattgcag ctggtcatga tcatcgcttt gtgcaaatcc
      541 ttggccagga attccatctc ggtgcggact ccatcgaccc ccccgccgat ggctgctcga
      601 atcatttcgc gcccaaccat tacgaaatcc gcccccaggg ccagcatctt gatcacatca
      661 aatccagtcc gcaccccgcc atccgcggtg agagtgactt ttcccttcag gatgggtacc
      721 agccggggaa ggacttcggc aattcccaac atcgaatcga gggtgcggcc tccgtggttg
      781 gagatactga ctactttgca ccccgtttcc agggcggcgt aagcgtcctc ctcgctcgtg
      841 atccccttga agatgaaagg caggctggtg gaccggacaa gctccttgag ctcggccacc
      901 gatttgcgga aaaccggctg ccctttcata gacatgggta cggaccctgc accgtccaca
      961 tctaccccta ccgctattgc ccccgctttt tcggcttcct gaatcagtcg gaagagcacc
     1021 ttctgctccc ggggcttgaa tatctgcacg ccccaccccg ccgcctcttt gatcgcctgc
     1081 aggccgggcc ggtcctcaaa agtttcggct ccgtcgccga tgaacccgat cgtccccgct
     1141 gccttggagc catggatcgc ggcgcgggcg aattccaatt cggtgatccc tcctcccatg
     1201 ctgattttga ccccactcat ggaggtgcag aagatcggaa aagccacctc tttccccaca
     1261 aaagaggctc tcatgtttgg ctctacatga ggggtgatca ggcgggtttt caatcttagc
     1321 cggtccagag ctttcacgtt ggcagcgaag ctttcccctt tgccgattcc ccccatcccg
     1381 atggctttgc cgtacttgat cccctggcag agcctttccg gcaaaccgtc gcagacctta
     1441 tagacgccgc agaacccttt catcttttct tttgctgttt cccgcacctc ggtcaaatcc
     1501 atgggattct cctttcacag cccatcaagg attccaaaaa caatgggcgc caaagttaac
     1561 ctatatatat tattctatgg gccctcatcc caatgtcaac tcaagccctt accgaagaga
     1621 gcatttctga caaaaaagtc aagcctttgc cagattccta ttcctcgtac ccgatggaat
     1681 aaaagaccgc ggaaaaaatt caagcgattc caaaaaagga gactcgggac atgggtacat
     1741 gcaaaaaaat acagcttttc ataacgaaaa atacacagtt cccccccttt ttctcttctc
     1801 cgttccaaaa tataaagaat aaataatgat tccacctgcg aaggaaggga aaaaagatgg
     1861 cgaagaatct cacccataag attttggagg ctcaccttgt ccagggtgag ttgatccgcg
     1921 gtaaggagat tgccatccgc attgatcaca ctctccttca ggatgcgacc gggaccatgg
     1981 cgatgttgga attcgaagcc ctgggaatgg aaagggttaa ggtggagctt gcggcgcagt
     2041 atgtagacca taacatcctg caaaccgata accggaatgc ggatgatcac aaatacttgc
     2101 agaccgcctg tgctcgctac gggatccgct tcagcccccc gggaaatggg gtttctcacc
     2161 aggttcatat ggagagattc ggagtccccg ggaaaaccct gcttggagcc gacagccata
     2221 ccacgggcgc ggcaggggtt tccatgctgg ggatcggagc cggagggctc gacgtggcct
     2281 tggccatggc cggttatcct tattttcttc cctgtccgat cgtggtcggg gtgaaactca
     2341 aaggcgagct tccggactgg gtgagcgcca aggatgtcat cctggaaatg ctgcgccgtt
     2401 ttggggttaa aagatgtgtc ggaaaaatcg tagagtatta tgggccgggg gtgaaaagtc
     2461 tttcggccac cgatcgggaa acgatcggca atatgggagc agaattgggt gcgaccagtt
     2521 cgatcttccc ttccgacgaa aacacccgag cctaccttga ggctcaagga cggggaaacg
     2581 cctggaagga attggctccc gacgaggggg cccagtacga cgaacatgag gagatcgacc
     2641 ttcgcgccct cgagccgctc atcgcccgtc caacctctcc ggggaatgtg gttgccgttc
     2701 gagaagtggc tggaatcaag gtcgatcagg tgattgtcgg cagcagtgtg aattcttctt
     2761 ttcgtgatct tatggttgtg gccaaaatca tggagggtcg acactgccac cccgaaacct
     2821 ccctgaacat aaaccccggc agccggcagg ttttggaaaa tatcgtttcc cagagcggag
     2881 ccatgtcttt gatgcacgcc ggcgcccgca tatatgaagc agggtgtcta gggtgcatcg
     2941 gaatggggca ggccccggga acgggacagg tcagcctgcg cactttcccc cggaactttc
     3001 cgggccgctc ggggaccaaa gaggaccagg tctatctctg ttccccggag acggccgctg
     3061 cggcagccct taaaggggtt atcaccgatc cccgggagct ggctaaggag atggcctacc
     3121 ctcgaatcca ggggccggcg agatacatca tcgacgaatc ttcagtcatc tttccttccg
     3181 atgacctgcg cgagaccatc gtgctccggg ggcccaatat caagcctctg ccccagctgg
     3241 acccgctccc ggaaaggctg gaggctgaag tcgtgctcaa agtcggggat aacatctcca
     3301 ccgataccat catgcccgcc ggcaataaaa tcctgccttt gcgttccaac atcgaggcca
     3361 ttagcgaatt tatcttttca ccgatcgacc ccaattttgc caaggaatgc agggaaaaag
     3421 gagggggggt cgtgatcggc ggtgaaaatt atgggcaggg atccagccga gaacatgccg
     3481 cgttggggcc ccgctacctg ggggttcggg ccaaaatcgt aaaaagcttc gcccgcatcc
     3541 ataaggcaaa cttgtgtaat tttggcattc tccccctgac ttttaaagac ctcagggatt
     3601 atgagcttct gcagaaagga tcgaaggtct tatttcccga cttgagaagg cgcgtggaga
     3661 aaggggaccg ggagattccg ctccaggtgg atgggaggac catgatctcc ctgctggagg
     3721 tctccgaccg ccaacgccgc cacctgctgg ccggtgggac cttaaactcg gtaagaaagg
     3781 aactgcaggg agggaagccg aaaagataag ggcgacccga tggagaagat ttgcctaagg
     3841 agggaattat ggctgaagcc gttagggtaa agaatattgg tctgcgggga gtaacggtag
     3901 ccgacaccaa gattagcttt atcgacgggg agcaggggat tctcatttac cgcggctacc
     3961 ggattgagga tttggcccgg aattcgtctt acctggaaac cgcctatttg cttctgaatg
     4021 gaaatttgcc ggacctacaa cagctcgaaa ccttcactcg acaagttgtt gaggccaggg
     4081 aaattcctaa attcatctat gatagtttaa agaaatggcc ccgggatgcc catcccatgg
     4141 atgtcctcca ggcatccata ccgattctgg ccatggccga ccccgatata catcaagaga
     4201 caagggaggc agcagtccgt caggcaattc gtctcatttc ccgcctgccc ggggtggtgg
     4261 caggctggca caggattcgg aagggccaga ggccccttcc tgcgaatgac cgtctctccc
     4321 atgccgctaa ttttttaaga cagctcaccg ggaagaaacc cgatggagag gttgcccatg
     4381 atctggatgt ctgcctggtc ctccatgccg accatacctt taatgcctcc acctttgcct
     4441 gccgggaggt cgtttccacc cgagcgcaca tgtacgcagc ggttgccgcc ggcatggggg
     4501 ccctctccgg gagccttcat ggaggggcca atactcaggt catgaagatg ctccaggagc
     4561 tctccggaga gaaagatatt gctgcatggg tgaaaaagca attggaccaa ggcgagaaga
     4621 tcatgggaat gggccatgca gtctataaga cgaccgatcc ccgggcgaac tttttgaagg
     4681 atatgggtct ccgtttgggc aaaaaactcg gccaggaaag gctgcccgaa atttccaggg
     4741 aattggaaaa agcagcggtc gaagagtttg aaaaacgagg gaatccgacc attaaaccga
     4801 acgtagattt tttcagcgct cctgtctacc acatgatggg cataccggcg gacatgatga
     4861 cccccgtttt tgccatcgcc aggatcgccg gatggtgcgc ccatatcatt gaagaagcct
     4921 ttgccgaagc ccaagaaaaa ccggccctat acagaccaaa atctgaatac gtgggaaatt
     4981 actgcggcct catgggatgt tcctatgaac cgctccaggc ccggaaatag gggggaaaga
     5041 cgggcagccc aggagggatc gctgcggcgt actcactttt tttctttcac cagctcgatg
     5101 atcttctgcg ccgcctcgcg catggacttg taggcgagga tgtttagatt ggagtcttcc
     5161 aggattttcc ttcccagttc cacgttggtc ccttccatgc ggaccaccag agggatggtc
     5221 aagggcctct cccgcagggc atccacgact ccctgggcgt agctgtcgca gcgggtgatc
     5281 cccccgaaca tgttgatgaa gatccctctc actttcgggt ccgagcgaag caggccgaag
     5341 gcattccgaa tcatgtcctt ggtggcgccg gccccggcat ccaggaagtt ggcggcctct
     5401 cccccgtagt acttcagcag gtccagggtg gccattccca ggccggcccc gttcacgatg
     5461 cagccgatgt tccccgtgag gttgatgtaa tttaagttca accgggaagc ctccacgtcc
     5521 cgcgggtctt cctgctcgat gtcccgcagg gccatcagtt ccgggtgccg gtagagggcg
     5581 ttgtcatcga tgttcatctt ggcatccaag gccaccaggt ctccccgcac agtcaaggcc
     5641 agcgggttga tctccgtcag ggaacagtcg cagtccatga aagcccggac cagcgcgctg
     5701 gttacggccg ccccctttcc gatcgccggg ccctgcaggc ccatgtccag ggccagcctg
     5761 cgaccctgga aattggtaaa cccgcacccc gactccaccg cgagcttatg gactttctcc
     5821 ggagttctgc ggctgacctc ctcgatttcc attccccctt cggagcagcc gatgaccacc
     5881 acctttccct gcttgcggtc gatggtcacc cccaggtaaa attctttttc aatcggaagg
     5941 gcctcttcga ccaggacccg gctcaccggc ttcccctgcg ggccgctctg ggcggtcacc
     6001 agccgcgtac cgaacatgga ccgggcgatc tcccggactt ccgccgggtc tttggccttc
     6061 cggatccctc cggcctttcc ccgcccgccc gcgtggacct gggccttcac gacgaagggg
     6121 cccatgccga tcccttgggc cgctgcttcg gcatcccgga ggtcgaagga aacatgtccc
     6181 tgggggacct tcaccccgta gcgaaaaaga atctctttgg cctggtattc gtggatgttc
     6241 atgggctccc cttggtcata ggttgggaaa ggaggtccgt aggggcgggt ttgaaatcca
     6301 cccctacgga caaccgaaaa ccggcaacgg gcttttagta tctttccccg accagcttct
     6361 tctttagctc cccgatggcc caggtgggat tcagggactt ggggcagatg gtgcagttga
     6421 agatggtgtg gcagcgccag agcccgtgac gggtgttcag aaccggcacc cgttcggcaa
     6481 atccctcgtc tcgggaatcg aagacatatc ggtaggcctt gagcagcgcc gccggcccga
     6541 ggaaattagg gttggcccag taggagggac aggaggaagt gcaggccccg cagaggatgc
     6601 attcgtagga gccgtcgatc ttcttccgat cctcgatgct ctggtaccgc tcctggtccg
     6661 tgggcggggt gccggcgatg agatagggtt tcacctcttc cagtttctgg aaaaagtcct
     6721 ccatgtccac cgccaggtcc ttgatcacct tgaagtgccg gagaggctcg atctcgacgg
     6781 tcccgccgcc cagggatttg acctgggtct cgcaggccag tttattgagc ccgttgatgg
     6841 tcatggcgca ggacccgcag atcccgtggc gacaggagcg acggaaggtg aggctgccgt
     6901 cctgtttcca tttgatctcg ttcagggtgt ccagaatggt catcccctcc tgaatgtcga
     6961 cctcgtaggt cttatagtag ggagccttgt cttcctcggg gttgaaccgg taaattttaa
     7021 agatttgcat catggtttac tccttcgaat ggttttctgt gatcggtctc acccccccct
     7081 gccctccccc gtcgaggggg agggattctc ttttataccc cctccccccg tggtcgggtc
     7141 taatcgactg gcgggagggg ggtgggggga gggggaagat aagggctaga acttcctctc
     7201 ctcgggcttg tatttggtga tcaccacggg cttgtaatcc agcctgatac cggtatcggt
     7261 cttccaggcc agagtgtgtt tcagccagtt cccgtcgtcc cgcttcggga agtcccggcg
     7321 gaagtgggcg ccccggcttt cggtccgcgc ctgaccgccg accacaatgg tctccacaaa
     7381 gtccaggatg ttgcccaact caatggcatc cagaagctcc gtgttgaacc gcttgccctt
     7441 gtagccgatg gagatgttct tgaaacgctc ctgcaatgat ttgatgatct ccagctgctt
     7501 cttcaggagt ttctcttccc ggaaaatcca gcagttgagc atcatgctct gctgcagttc
     7561 atcgcggata gccgcggggc tatccttccc gttgaggctc aggtaccagt cgatcttggc
     7621 tttggccttt tccagcccgt ctttggggag gtccggtttt tccgcccccc cgaggatata
     7681 cttgaccatg gtccggccgg tcctcctgcc gaaaacggca cagtccatgg tggagttgca
     7741 gcccagccgg ttggcgccat gaacggagat gcaagcgcat tccccggcgg catagaaacc
     7801 ggtaacgggg gtattcttgg catccatgat cacctgaccg tccacattgg ttggaatccc
     7861 gcccatggcg tagtgggccg tgggctggat ggggacaaaa ccggttaccg cgtcgatgcc
     7921 ggagaacttg aggatgagct cgtgaatctg gggcagcttt tccatgatgg cctcgcgccc
     7981 caagtgccgt aagtcaagat ggacatagca tttcccgtcg attccccggc cttcgtcgat
     8041 ctcctgctgg atagagcggg agaccacgtc acggggggcc agctccatct tggccttggc
     8101 gtatttctcc atgaaccttt tgccctcatg attgaccagg tagccgccct cgccccgggc
     8161 tccttccgta accaaaactc cggaggggta gagccccgtg gggtggaact ggacgaactc
     8221 catgtcctcc agcggcagtc ccgcttgcag gaccagggac attccgtctc ccgtgttgga
     8281 aagggcgttg gtgttcacct tccaggcccg ggcgtatcct ccggtgccga acatgaccgt
     8341 cttggcatgg aacacctgca ggccgccgtt gcgcatatcc caggcgacca cgccggagca
     8401 cacgtttttc tccatcagga ggctgacgac gaagaactcc gagtagaaac gcacgtcgtg
     8461 cttgatgcac tgttcgtaga gcgtatgcag catggcatgg cccgtccggt cggctgcgta
     8521 gcacatccgg cggatggccc ctcccttgcc gaagttgctg tagtgccccc cgaattcccg
     8581 ctgggcgacc ttgccctggt ccgtccggct gaaagggcag cccatgtgtt ccagctcgta
     8641 agccacctca atggcatccc ggacgagcac ttcctgggca tcctggtccc ccaggtagtc
     8701 ggcccccctc accgtgtcgt ataggtgcca ctcccagtgg tcttcctcca ggctccccag
     8761 cgccgcagcc gtgcccccct gggcggaaat agagtgggac cgggtagggt agactttcga
     8821 tatgaccgcc gtattgagat gcttgctgga ttccagggcg gcatacaggc cagcgatccc
     8881 cgccccgaca atcaccacat cgtgttcatg aaccatctga tcctccagtt ttctaaaaac
     8941 attttcacca cggaggcaca gaggacacag aggaaaaaga acctaagaat gtaaaaaacc
     9001 aagctctttt tgcttttggg ggttattcac cttaattttc tcagtgttct ctgtgtctct
     9061 gtggtgaata gatgtctctt acgtgccgaa aggaaccaaa gtgacgaatc ccaggatcaa
     9121 gaaggagacc cccaggatcc aggccactcc aaagagggtc acccgaagcc cgttgcattg
     9181 cacatagtcc tccaccatca gccagaaccc gttcaggatg tggtagagga gcatgatcaa
     9241 gaaccccacg tggaagaatt tccagaaagg acttcccagg tccacggggg aggccccgaa
     9301 atggacggct cccaagtgga tgattaggac gacggcgatg agaattcccg tgatcctctg
     9361 aaaaagccag tgaaaagcac cggattgatg agatgcaggt aaggccattt ctttttcctc
     9421 ctagatcgct ctcaggccgg ccacaaagaa gaccagcccg atgaccatca tcgcccagaa
     9481 gagcttcttg tggtaaagag ccccgcttcc aaaatcgatg atcagttccc ggattccgtt
     9541 cagagcgtgg tagatcagga aggccaggag aatcaccacc accaccttgt gggtcaggaa
     9601 gttgctgatg cccgccagcc ccttctgatc gtacttcatc ccgaactggg ccaggatgtg
     9661 gagcaggaga tagatcccca cggcggcccc ggtgatgcgg tggatcaacc aggccgtagc
     9721 cccggtttgc catttgtatc gcatctattt tctccttcaa ttttactggg acgcagatct
     9781 acgcagaaag gccggataaa taatattgat gaaatcgtaa aaagctccta gaaccgtcac
     9841 cccggcgaaa gccggggtcc agaatgtgtt gaaataactg gattccggct ttcgccggaa
     9901 tgacgcatct tacggaattc cgccttttga cgagtacatc aatgttcatt tccaaaatcc
     9961 tgtaaatctg cgttcatctg cgtcccattc gttttttacc gcgcgtattg cgccatgccc
    10021 tgctggtaca ggtcattccc cttggtgtca ttgaccacga ttgccgggaa gtccaccact
    10081 tccagccggc tgatggcttc tggtccgagg tcctcgtagg ccacgatctc ggccttcttg
    10141 acggatttgg ccaagagcgc tcccgccccg ccggtggcgg cgaagtagat cgccttgtat
    10201 ttcttcatgg cctcgatgac ttccttcttg cgcatgccct tgccgatcat ccccttgagg
    10261 ccgatctcca tcagcttcgg cgaataggcg tccatgcgcc cgctggtcgt cggcccggcg
    10321 gacccgtaca catgccccgg tttcgtcggg gccggtccca cataatagat aatctgccct
    10381 ttcaggtcga tgggcagggg tttcccctct tttaacaggt cgaagaggcg tttgtgcgcg
    10441 gcgtccctcc cggtgtagat caccccggtg atcaggactt tatccccggc ttttaatttt
    10501 tcaacgtccg cgtcgctcag gggtgtcttc aatcgaatcg cttggctcat tctgcaacct
    10561 ccttcagatg acagcttctt ggtgccggtg ggcgtggcag ttcaagttta cggccatggg
    10621 aaagctggca atgtggcagg ggatcaattc cacatttacc gccagggcgg taatccggcc
    10681 tcccaatccc tgaggtccga tgcccaggtt gttgatccgg gtgaggagct ctgcttccat
    10741 ggccgccagc tgcgggtccg gattcttctg cccaaggggg cgcagaagtg ctttcttggc
    10801 cgtcagggcg acttcttcaa aagttccccc gacacctact cccacgacca ccggagggca
    10861 tggattggaa ccggactgtt tcacccattc caccacccgg ttcttgactc cctcaattcc
    10921 ctccgagggg ctaagcatga tgaccgtgga catattctcg gctcccccgc ccttcggggc
    10981 caggacgatc ttgatcttgt cgccggggac caggttcaaa tggatgatcg ccggagtgtt
    11041 gtccttggtg ttgacccggg tgaaaggatg gcaggaggat ttgcggagat acccttcctt
    11101 gtagccctgc cggacccctt cgtggatggc ttcactgaag ctcccgccga caatgtgcac
    11161 ctcctggccc aggtctatga acaggaccgc caggcccgta tcctgacaca tggccacctg
    11221 ctcttcccgg gcgatgcgga cattctcctt gatctgctgg aagacttcct tgcccacggg
    11281 ggattcttct ttcttctccc cctcgtccag cgccttgaga acatcctcgg tcaggtagta
    11341 attggaatcg atgcacatct ttttcacgac atcggtaatc tgtttggcat tgacttctct
    11401 catcccttcc tccttctgaa tccaagagag agccttcggg gacgctccgt ttttccctcg
    11461 gtccgggggc ctacggaagt ccccgaatgg ccctggggat cagatcttgc actccggaat
    11521 gatcccgctg ttcatcatca gctcgacctc tctccgcgcc cggtcgatca tggcagaggc
    11581 tttctcgaag agctgctcct tgctcatctt gacccgggcc accttctgct cgatgccctt
    11641 gaggcccacg gccacagcct gacgggggaa gacctcgcgc tcactcatta aggggagcag
    11701 gtaatccttc ttgagtccct tgtcttcggc gcacttggcc aattccaccg ccgaggcgat
    11761 gcacatttcg tcggtgatgg tggtggccat gatgtccagg gtgccgcgga aaatggccgg
    11821 gaagcacagg gagttattca cctggttggg aaaatcggaa cggcccgtgg cgatgacctt
    11881 ggccccggcc tcttccgcct cccaggggta gatctcggga atgggattgg cgcaggcgaa
    11941 gaggatggaa tctttagcca tcttcttgac ccattctttc ttgatcacgt cagggccgga
    12001 tttagccagg gccacgacgg cgtccgcccc ctccatggcc tcggcgatgt tccccttgcg
    12061 ctgctccgcg ttggtaatct gggagaaccg cctcttttcc gcgtagtccg gggggatatc
    12121 cgttcttccc ttgtgcagcg tccccttgga atccaccatg atgagcttgg cggggtccac
    12181 gccgtaggag agcatcactc gcgcgcaggc tatattcgct gcccccgatc cgatgagggt
    12241 gatgcgcgct tttttaatat tcttcttcac taccttgaag gcgtttatca accctgcaag
    12301 ggtcacggct gcggtgccct gctggtcatc gtgccagacc ggaatcttgg actctttacg
    12361 gagggtatcc aggatatgga agcacttggg ctgggacagg tcttcgaggt taatccctcc
    12421 gaagcagggg gagatgagct ttacggtttt gatgatctcc tccgcatcct tggtgtcgag
    12481 gcaaaccggc caggcatcca ctcctcccag gtatttaaag aggatggatt tcccttccat
    12541 gaccgggatg gccgcttccg gcccgatgtc tcccagcccc agcacgcggg tcccgtcgga
    12601 aaccaccgct accatgttgc ctttgttcgt gtgctcgaag actttttccg gatgggcttc
    12661 aatgtcccgg caggcagcag ccacgccggg agtgtaccag atggcaaaat cattgaaatc
    12721 ccgcacgcag catttcactg ccgaggcaat cttgccctta taataagggt gcatcttcaa
    12781 cgcgtctgct gaaggtttct tggatttttc caacagttgt tctttggtca gcatgtcttc
    12841 ctccttttga ttcttgccgc attctctgct gcaagggtat gagtttgaaa attcccccct
    12901 tcctttttcg gaaggcaatg ggttagcgtt cgctcaactc taaaagtacc ccgttggttg
    12961 atttgggatg gatgaaggca atcttcgctc ctccagcgcc tctcctcggt ttctcgtcga
    13021 tcagtcgcac ccccttttct ttcagttctt tcaaagcttc ttcgatattg tccactcgca
    13081 gggcgatgtg ctggattccc tctcctcggc tttcgatgaa cttggcgata gccccatccg
    13141 gcgccgtgga ctccagaatc tccagctcgc tctcccccac cggaaggaaa gcggtggtgg
    13201 ctttctgctc ctgcaccgtt tcccttcctt cgatgggcag ccccagaatg acgttccaga
    13261 aaggcgccac ctgctcgatg gacttggccg caatgccaat gtggtcaatc ttcaatattt
    13321 tcatccttcc tacctccaaa agaattttct acttcgatcc tcgaacctcg ggccttaaac
    13381 cttcaccgcc gattcgtact ctccgaacac gctccgcagg gcgtcgcaga cctcgcccag
    13441 agtgcagtgg gtccggacgc agctgaggat ggggaccatg aggttgcctt ctccccgggc
    13501 gacttttttc agcgcttcca gggaggtctt gaccccctgg gaactgcgcg aggttttgag
    13561 ttcggcgatg cgtttgatct ggacttcccg gaccttgggg tccaccttca gcagtccctt
    13621 gggcggaggt tccttggatt ggaaccgatt cacccccact atgacccggt ctccgctttc
    13681 gatctccttc tggtagcggt aggccgcgtc ctggatttcc tgctggatgt atcctttttc
    13741 gacggccacc accgacccgc cgagctggtc gatcttgtcg atgtaatcca tggcccgttt
    13801 ttcgatttcg tcggtcagtt tctcgatgta gtaggacccc cccaggggat cgatggtatc
    13861 cgccgaaccg gattcatggg cgatcagttg ctgcgtccgc agagcaacct gaaccgactg
    13921 ctccgacggg agagccaggg cttcatccat agagttggta tggagggatt gggttccccc
    13981 gagaacggcg gccagagcct gccacgccac gcggatgatg ttgttgtagg gctgctgggc
    14041 ggtgagggtg gacccggccg tctgggtatg gaaacgaatc atccaggacc gcgggttttg
    14101 ggccttgaac cggtccctca tgattttagc ccagagtcgg cgggcggcgc ggtatttagc
    14161 cacctcttcc agaaaatcca ggtgggcgtt gaagaaaaag gagatgcgcg ggccgaattc
    14221 atcgacgtcc agcccggctt tgattgccgc ctgaacgtag gagacgccgt tggccagggt
    14281 gaaagccact tcctgcacgg ccgtgcaccc ggcctcccgc atatggtacc cgctgatgct
    14341 gatggtgttc cagttgggga cctccttggt gcagtactca aaggtgttgg tgatgatgcg
    14401 catggaaggc cggggaggaa aaatataggt cccccgggag gaatactcct tcaggatgtc
    14461 gttctggatc gtcccgttga gcttggctgc ggcgacgccc tgcttctctc cgacagcgat
    14521 atacatggcc aggagaatcg aggccggggc gttgatggtc atggaggtag agaccttgtc
    14581 cagagggatt tggtcgaaga gaatctccat gtccttgacc gaatcgatgg ccacccccac
    14641 ctttcccact tcgccctggg ccaggggatg gtcggagtca tagccgattt gagtgggtaa
    14701 atcaaaggcg acggaaagcc cggtctggcc ctgttccagc agatatcggt aacgtcggtt
    14761 ggtgtcctca gccgtcccga atccagcgta ttggcgcatg gtccagaacc gtccccggta
    14821 catggtgggc tggacacccc gggtgtaggg gaactctccg ggaaagttca ggtcctgctc
    14881 gtaatccaat ccggccacat cttcaggagt gaatatccgc ttcacgggaa tgcccgaggt
    14941 ggtttgaaag gtcttcctcc gctccgggtt tttggccaga gtcttctcga tcttcccatt
    15001 ccattccttg gcctgcttgc caatctcctc ggactttttc ggatcgaaca tggaaacccc
    15061 cggtaagagc gcaaaaaatc gagaatccag catccaggag tcagaataaa agcctttctt
    15121 ctggattctg acttctgagt tctgaattct atcttttcta actccgaact ccgaactctg
    15181 aaccccgaac tgtttttcct cgaatcctcc ggatgacttc ctgaacttgg ctctggagca
    15241 gataatcgat gatttcctcc cggctgtatc gaggcttcaa gatccagcgg cgcagaacgc
    15301 caaagacgcc gctgtagacc agaaaattgg cttccaggaa agggtcgaac gggcctgggc
    15361 ccagctcgcc gatgatcttc tcaaaatgct cgacgatctc ggattctttt ttcaaaatga
    15421 ttttgaggta atccttctgt acatagcggg cctgggtgta cgaaagcatc acctgatctt
    15481 ccaattccat ggccacttcc aggaatgccc gataggtaat ctccagccgt tggagcgggt
    15541 cggcaaccat ggaagcccgg gacctcacct tttcgaaaaa agtattggca tagttttcaa
    15601 aaaaaagata gaggatatca tctttggaat tgacgtagtc gtagatgctc cccaggccga
    15661 ttcccgcttc tctggcgatc tcccgcaccg tagctttgtg gtagcccttc ttcttgaaga
    15721 cctggatggc cccttcgaga atctggctgc gcttcccgct gattaagctt tggtccttga
    15781 ctcgggcttg aacctgcatg acccctatct ggtaaggaag gtgatcaaag gtttagagcg
    15841 aacgaatgtt cattcatc
//
LOCUS       DASPRR010000434         4930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_249949, whole genome shotgun sequence.
ACCESSION   DASPRR010000434 DASPRR010000000
VERSION     DASPRR010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249949"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..494
                     /locus_tag="VEL68_15975"
     CDS             78..494
                     /locus_tag="VEL68_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXZ37520.1"
                     /translation="MAQEMPKEKRDSRTYSIIGAAMEVHRQLGCGFLESVYQEAMALE
                     MTGRGIPYGREVEIPVFYKNQRLNTSFRADFLCFDLVIVELKALGSLSGIEEAQLINY
                     LKATGYEVGLLFNFGAASLEHRRLAFSKPLPSIKSA"
     gene            complement(478..1659)
                     /locus_tag="VEL68_15980"
     CDS             complement(478..1659)
                     /locus_tag="VEL68_15980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ37521.1"
                     /translation="MIKINERIMITVVPFWMDLSASVFYLAAPLVVIELGGNPIELGL
                     IGTISASVHMVLAHLAGRLSDRFGRRALMVAGPFIFGISCLLMTQIGSVNAVLALAAL
                     NGFSMAVFWPAFQAWIAESQNGSGLARNIGSFNMSWTASHLIGPTLSGLLFGLHSRLP
                     FWLAGALCISLFLVMRVSLTERTSQPQKATESLPSESEDTNQGRTFLYAIWIANFASF
                     FILANARYQFPKLARELTMSPYMIGVLMSCIGLAQFLGFFFLRSSDRWHFQKIYLLGA
                     QLLATFGLVLIFFFNQQLVFASAFIMLGLCASLTYYSSIYYTVRLMKKKGKGAGLHES
                     ILGSGVVLGPLLGGILAHSAGLRAPYILCLAVLAIVVIIEVSLIKKNPHPQITQITQI
                     L"
     gene            1920..2576
                     /locus_tag="VEL68_15985"
     CDS             1920..2576
                     /locus_tag="VEL68_15985"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXZ37522.1"
                     /translation="MEILEVLKKGRIVELNKVLEPGKEGRRLEIRKFRIFAGEFMHDI
                     DTMSHIGTHAEAPSHFIPALPDGKDAPDIAEFPPDAWWGEAIFINLAKLPPKAKVTPE
                     LLKQKGIKKGDIVVIGNSPYKDDDKITMTDAAAKYLAGVGIKLLAMDFSYSIEENFSE
                     LGKMRVHVDLMSLGVPLIEGLAHLDQIKEERFFFMGLPIRVKGCDAWPIRAIAIEGVL
                     "
     gene            2655..3344
                     /locus_tag="VEL68_15990"
     CDS             2655..3344
                     /locus_tag="VEL68_15990"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXZ37523.1"
                     /translation="MNLKSISKKDPPRVALVSSTRAVFAPMEAAFREVFPEAQTLHLL
                     DETLIEDFRREGGLSSHSRHKALQMALTAQEAGVDGILVTCSTLSPSVDDFRPFVRIP
                     IIKIDEPVIEEVVQKADTIGLLATAETVLKSVEPLVMKKARDSGRKISVHPFVKGDVW
                     PLLQKDPSAFYRAIAEAATQAAQECQAVILTQVSIAPGRDYVEQKLQNKIYASPSYAV
                     QTLRKILSKSE"
     gene            complement(3538..4374)
                     /locus_tag="VEL68_15995"
     CDS             complement(3538..4374)
                     /locus_tag="VEL68_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ37524.1"
                     /translation="MVKMLRQVRLGVIAAVIILWTCLPIFHMVILSLTPQSAAFAGRI
                     WPDNPSLENYRSALKQGHPFLSHFWQQLLNSLLVAFATCALVLFIASITSFALGRLRP
                     RWAPVISNMALFTYLIPAAFLAIPLYRVMGQYRILNHPWSLILALVAFATPYAIWVMR
                     QYAESVPYELDESARVDGATVPQIFWHIYIPLIAPSLVAIGTYALLLAWNEYLYAFLF
                     LSSEAKLTVPVMLGHFTTSDDAPWTLMMASAVIYSLPPIALYYSVRRFMVTGLTAGSV
                     KS"
     gene            complement(4376..>4930)
                     /locus_tag="VEL68_16000"
     CDS             complement(4376..>4930)
                     /locus_tag="VEL68_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ37525.1"
                     /translation="IFLLPWAAPSLPAIFSIRWIFNSEWGMINGILFRLFGIDGPLWL
                     VKPDLAFGLVIATHIWKNLPFWTLILLAGRMAIPKDHYEAANVDGATRFQAFRFITFP
                     GIKNLYITSTLLSTIWSVGDFNSVYLLTGGGPWDATHVLSTLGIRYAFNIGDIPTGVA
                     TVITALPLVVPIVFVLIRRLGKGE"
BASE COUNT         1273 a         1243 c         1406 g         1008 t
ORIGIN      
        1 atccgcagat tacgcagatt tcacggatta atatgaggca aaaagacttt catcattcta
       61 aaaaagagga tagtctcatg gctcaagaaa tgccgaaaga aaagagggat tctcggactt
      121 attctatcat cggggcagct atggaagttc atcggcaatt gggctgcggg tttctagaat
      181 cggtttacca ggaagccatg gccttggaaa tgacgggacg ggggatccct tacggccggg
      241 aggtggagat tccggtattc tacaaaaatc agcgtttgaa tacatctttc cgggctgact
      301 ttctttgttt cgatttggtc atcgttgagc tcaaggccct agggagcctc agcggaatag
      361 aggaggctca actcatcaat tacttaaaag ccacgggata tgaagtcggc ttactgttca
      421 atttcggagc tgcatccctc gaacatcgcc gattggcctt ttctaagcct ttaccctcta
      481 taaaatctgc gtaatctgcg taatctgcgg atgcggattc tttttgatga gacttacctc
      541 aatgattacc acaatggcca ggaccgctag acagagtatg taaggagccc tcagcccggc
      601 ggaatgggcc aggatccccc cgagaagggg tcctaatact accccgctcc ccaggatgga
      661 ttcgtgcagg ccggccccct tccctttttt cttcataagg cggaccgtat agtaaatact
      721 ggagtagtac gtcagactgg cacagaggcc cagcatgata aaggcggagg caaagaccag
      781 ctgctgattg aagaaaaaga tcaacaccag tccgaaggtg gccagcagct gagcccccag
      841 gaggtaaatc ttttgaaagt gccagcgatc gctgctccgc agaaaaaaga acccgaggaa
      901 ctgggccagg ccgatacaac tcatgagtac cccgatcata taagggctca tggtcaattc
      961 ccgggccagc ttcggaaatt ggtatcgggc gtttgccaaa ataaaaaagg aagcgaagtt
     1021 ggcgatccat atggcgtaga ggaaagtcct tccctggttg gtgtcctcag actcagaagg
     1081 caaagattcg gttgcctttt gcggctggga ggtcctctcg gtcagagaaa cccgcatgac
     1141 caagaagaga gagatgcaaa gagctcccgc cagccagaaa ggaagacggg aatggaggcc
     1201 aaaaagaagc ccggacaggg tggggccgat gaggtgagag gccgtccagg acatattaaa
     1261 gcttccgatg ttccgggcaa gccccgaccc attctgcgat tcagcgatcc aagcttggaa
     1321 agccggccaa aaaacagcca tgctgaaccc gttgagggcc gccagggcca ggacggcgtt
     1381 aacactcccg atttgggtca tcaggaggca cgaaatacca aagataaagg gtccggcaac
     1441 catgagcgcg cgccttccaa accgatccga aaggcggccc gccagatgag ccaggaccat
     1501 gtggaccgag gctgaaatcg tcccgatcaa acccagctca atcggattcc ctccaagctc
     1561 aatcactacc aggggtgcag ccaggtaaaa aacactcgcg ctcaggtcca tccagaaggg
     1621 cacgacagtt atcattatcc gttcattgat cttgatcatg caaagcgaac atccttccac
     1681 gaggctcaaa agccccttgg ttcaaccttt cataatgaag cagtgatttt ccgatctggt
     1741 gggtccagga tacgttattt caagcctcct ttcaaataga aatagaattg atgccctaga
     1801 ggaattcgaa ctcgcttttt tagttgaaca taaatccatt cggctttagg atagaagaaa
     1861 aatatccctg gctagttcgc gaaattcaag aggataaaat ctaactgaaa ggatggagga
     1921 tggaaatttt agaggtactg aagaaaggaa gaatcgttga gctgaacaag gttttggaac
     1981 cggggaagga aggaaggagg ctggagattc gcaagttccg gatttttgcc ggtgagttca
     2041 tgcatgacat tgataccatg tcccatatcg ggacccatgc ggaggctccg tcgcatttta
     2101 tccccgccct gcccgacgga aaagacgctc cggatatcgc cgaatttccg ccggatgcct
     2161 ggtggggaga ggcgattttc atcaacttgg ccaaactccc ccccaaggcc aaggtcaccc
     2221 cggaactgct caagcagaag ggaatcaaaa agggcgatat cgtggtcatt gggaattccc
     2281 cttacaagga tgatgacaag attaccatga ccgatgcggc agccaaatac ctagctggtg
     2341 tgggaatcaa attactggcc atggacttct cgtatagcat agaggagaac ttctccgaac
     2401 ttggcaagat gagggtacac gtcgacctga tgtccctggg tgtcccttta atcgaagggc
     2461 ttgcccacct ggatcagata aaggaggaaa ggttcttctt catggggttg cctatccggg
     2521 tgaaaggctg cgatgcctgg cccatccggg cgattgcgat tgaaggagtt ctttaagaga
     2581 ctccggagaa tgtagtaaag ctattttctt aagtgacgtt tatatctctg ttctctgcgt
     2641 aatctgcgga taagatgaat ctcaaaagta tttctaaaaa agacccaccc cgggtggccc
     2701 tggtttcgag cacccgagcg gttttcgcgc ccatggaggc agcgttccgg gaggtgttcc
     2761 ccgaagccca gactcttcat ctcctcgacg agaccctgat cgaagacttc cggcgggaag
     2821 ggggattatc gtctcattcc cgccacaaag ctctgcaaat ggctttgacc gcccaggaag
     2881 ctggggtgga tggcatcctg gtgacctgtt ccaccctttc cccctcggtg gatgactttc
     2941 gtcctttcgt gagaatccct ataatcaaga tcgatgagcc ggtgatcgaa gaagtggttc
     3001 aaaaggcgga taccatcggc cttctggcca cggcggaaac ggtgttgaag tccgtggagc
     3061 cgctggtcat gaaaaaagcc agggattccg gccgaaaaat atccgtccac ccattcgtca
     3121 agggggatgt ctggcccctc ctgcaaaaag acccgtcagc tttctaccgg gccatagcgg
     3181 aagccgccac ccaggccgcc caggaatgtc aggccgtcat cctgacccag gtttccatcg
     3241 ctcccggcag ggattatgtg gaacagaagt tgcagaataa aatctacgcc tccccctcct
     3301 atgccgttca gaccctccga aaaatccttt caaagtcgga atgaaaatgc attaaatttt
     3361 ctctcgcaga ggccgcagag aacgcagagt ataaaaagat tcaggtccca ggtctcagga
     3421 ttcaggttcc taactctgat tcctggctcc tgtttcctgc cttattttct ctccggaccc
     3481 tgcgggcccg agcggagcag acgagagaag tcccacgctt attcgggaat tgtggtttca
     3541 ggacttcacg gaacccgcgg tgaggccggt caccatgaag cggcggacgg aatagtagag
     3601 agcaatcgga ggcagggaat agatcaccgc ggaagccatc atcagcgtcc agggcgcatc
     3661 gtcggaagtc gtgaaatgtc ccagcatgac cggaacggtg agcttggcct cgctggagag
     3721 gaacaggaac gcgtacaaat actcgttcca ggccaggagc aaagcatagg tcccgatggc
     3781 cacgagggag ggcgcgatca gcgggatgta gatatgccag aagatctggg gcaccgtggc
     3841 gccgtctact cgcgcgcttt cgtccagttc gtaggggaca ctttctgcgt actggcgcat
     3901 gacccagatg gcgtaaggag tggcaaaggc aaccagggcc aggatcaggg accagggatg
     3961 gtttagaatc ctgtattgcc ccataacccg atagagcggg atggccagga aggccgcggg
     4021 gatcaggtag gtgaaaaggg ccatgttgct gatcaccggg gcccatcgag gacgtaatcg
     4081 ccccagggca aaggaggtga tgctggcgat gaatagaact agggcgcagg tcgcgaaggc
     4141 caccaatagg ctgttgagca gctgctgcca gaagtgggac aggaaagggt ggccctgctt
     4201 caaggcggac cggtaattct ccagggaagg gttgtcgggc cagattcgcc cggcgaaagc
     4261 agccgattgc ggagtgaggg acagtatgac catgtggaag atgggcaggc acgtccataa
     4321 aataatgacc gcggctatga ctcccagacg gacctgacgc aacattttaa ccatttcact
     4381 cccccttccc cagccttcgg atcaggacga aaacaatggg aaccaccagg ggaagcgcgg
     4441 tgatcaccgt ggccacccct gtgggaatgt cgccgatgtt gaaggcataa cggataccga
     4501 gagtggagag gacgtgggtg gcgtcccagg gtccgccgcc ggtgagcagg tacacactgt
     4561 tgaagtcgcc cacactccag atcgttgata gaagggtact ggtgatatag aggtttttga
     4621 tccccggaaa ggtgatgaac cggaaggcct gaaaacgggt cgccccgtcc acgttggcgg
     4681 cttcatagtg gtctttggga atggccatgc gcccggccag gaggatgagc gtccagaagg
     4741 gcaggttttt ccagatgtgg gtggcgatca ccaggccgaa agccaggtcg ggcttcacca
     4801 gccacagcgg cccgtcaatg ccgaaaaggc gaaagaggat tccattgatc atgccccatt
     4861 cgctgttgaa gatccagcgg atggaaaaaa tggcaggcaa ggagggagcc gcccagggca
     4921 gaaggaaaat
//
LOCUS       DASPRR010000435         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_756679, whole genome shotgun sequence.
ACCESSION   DASPRR010000435 DASPRR010000000
VERSION     DASPRR010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756679"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VEL68_16005"
     CDS             complement(<1..302)
                     /locus_tag="VEL68_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37526.1"
                     /translation="MSLARQTGARIPVVELFALFHDARRMNDGWDREHGYRGAELAAQ
                     FRRTHFHLLDSDFDLLYEACRDHTLGSTTADITVQTCWDADRLDLGRVKIDLDRKYL"
     gene            complement(632..1681)
                     /locus_tag="VEL68_16010"
     CDS             complement(632..1681)
                     /locus_tag="VEL68_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXZ37527.1"
                     /translation="MMDDRLSKTLKVITGQDLGGDAQGRCEIKEAGKSDVDLLDAYSR
                     AVIHVAEAVGPAVVGISIGKEIGEEAFTKTGAGSGVVIAPDGYILTNDHVVHQADQLT
                     ATLADGSKLHAALVGTDPGTDLALIRAVASNLPYATLGDSSLLRVGQLVIAIGNPFGF
                     QSTVSTGVVSALGRALRSPQGRLIENIVQHTAPLNPGNSGGPLVDSRGRVVGVNTAII
                     AIAQGIAFSIPSNTAKWVVSQILSHGRVRRAYLGIAARQRSLNRRLVRFYKLAKEEAV
                     EVISVDPQGPAGRAGMKEGDFILAVNDQAVTSVDDLHRFLAEGFIGKMVTMSVFRGQD
                     KFQLEVIPGEASPNG"
     gene            complement(1736..>2476)
                     /locus_tag="VEL68_16015"
     CDS             complement(1736..>2476)
                     /locus_tag="VEL68_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HXZ37528.1"
                     /translation="GAEGFKAGDRVFLTGTVSGAYAEKTLCRDHQVHPLPEGVSFSQG
                     AAVNTPYSAAYHALFHRARAIPGEWVMVHGATGGVGIAAVQLARAAGMMIIGTGGTER
                     GRRLIAEQGAQHVLDHRAPDYLDLALKLTQERGVDVIVEVLANVNLGKDLPVLAHGGR
                     VVVVGSRGTVEINPREVMIRQAAILGMLVANASERETRSIHSALTAGLENGTLRPVIG
                     HELSLLEAAQAHRKVLEPGAFGKIVLIP"
BASE COUNT          502 a          787 c          660 g          527 t
ORIGIN      
        1 agatatttgc ggtccaaatc gatcttcacc cgccccaggt ccaggcggtc tgcgtcccag
       61 caggtctgaa cggtgatgtc tgccgtggta ctcccaaggg tgtggtcccg acacgcctcg
      121 tagagcaagt caaaatcgct atccaagaga tggaaatggg ttctgcggaa ctgggcagcc
      181 aactccgctc ctcgatatcc atgttcacgg tcccatccat cgttcatcct ccgggcatca
      241 tggaaaagcg caaaaagttc gaccactggg attctggctc cggtctgtcg ggctaaactc
      301 atcccatttt ccaggaccct cgcccagtgg ggcaagccgt ggatgccctt cggagaaagc
      361 gaatattgcc ggaggattgt ttcgatcaaa tctttagaaa tcataggcaa agtagctcaa
      421 tgccgattca tgaatcgggt tcgcaaggat aatcatggcc agtatacgtt aggctttttc
      481 actaggtcaa atgttcaaat tgcagggtct tcccttcccc tgaagcccga gacccagggg
      541 gaggccggtt ttcgttcttg ggtcagcgac aatagaggac ccgttctgaa gacactttca
      601 tccgcgaggc aggaggaggg aatgtcgggt tttacccgtt cggagaagct tctcccggga
      661 taacctccaa ttggaatttg tcttgtccgc ggaagacgct catggtaacc attttcccga
      721 tgaacccttc ggccaaaaag cggtggaggt catccacact ggtaacggcc tgatcgttta
      781 ccgcgaggat gaaatctccc tccttcatcc ccgccctccc cgcaggtccc tgcgggtcta
      841 cggagatcac ttctactgcc tcttctttgg ccagtttgta gaaccggacc agacggcgat
      901 tcaatgaccg ctggcgggcg gcaatcccca gataggccct ccgaacgcgg ccgtggctca
      961 agatttggga aaccacccac ttggcggtgt tggaggggat ggagaaggca attccctggg
     1021 cgatggcgat aatggccgta ttcactccca caacccgacc gcgggaatcg accaaggggc
     1081 ctcccgagtt ccccgggttc agcggggcgg tatgctggac aatgttctca atgagccttc
     1141 cctgcgggct tcgcaacgcc cgacccagag cgctcacgac tccggtcgaa accgtagatt
     1201 gaaatccgaa agggttgccg atggcgatca ccagttgtcc gacccgcagc agagaagaat
     1261 cccccagagt tgcataggga aggttggaag caacagcccg gatgagggcc aagtctgtcc
     1321 ccgggtccgt tcccaccagg gccgcatgaa gcttcgagcc atccgcgagg gtcgcggtta
     1381 actggtcagc ctgatgcacc acatgatcgt tggtcaggat atacccgtcg ggagcgatga
     1441 ccacccctga accggctccc gtcttggtga aagcttcttc cccgatctcc ttcccgatgg
     1501 aaatcccgac caccgccggt cccacagcct ccgccacatg gattaccgca cgggagtaag
     1561 cgtcgagaag gtccacatcc gattttcctg cttcctttat ctcgcagcgg ccctgcgcat
     1621 cgcctcccag gtcttggccg gtaatcacct tcagcgtctt gctcaatcga tcatccatca
     1681 tcgactcgct ccttttctcg cctcagcctc tctccccctg cttccagccg cctttttaag
     1741 ggatcagtac gatcttgcca aaggcccccg gctcaaggac ttttcgatgg gcctgggctg
     1801 cttccaggag agagagctca tgcccgatga ccggtcgcag cgtgccattc tcaagccccg
     1861 cggttagggc ggaatgaatg ctccgggttt ccctctcgga ggcattggcc accagcatcc
     1921 ccagaatagc cgcctgccgg atcatcacct cccgggggtt gatctccacc gtcccccggc
     1981 tgcccaccac aaccacccgc ccgccatggg ctagcaccgg aaggtccttt cccaggttca
     2041 cattggccag aacctccaca atgacgtcca cccccctctc ctgggtcaac ttcagggcta
     2101 aatccagata gtccggagcg cggtgatcga gcacatgttg ggccccttgt tcggcaatca
     2161 gacgtctgcc tctttcggtc cctccagttc cgatgatcat cattcctgcc gctctggcca
     2221 gttggactgc ggcgataccc acgcctccgg tagctccgtg gaccattacc cattctccgg
     2281 gaatcgcccg ggcccggtga aagagggcgt ggtaagctgc cgaataaggg gtgttcaccg
     2341 ctgctccctg ggaaaaggaa accccctcgg gcagcggatg aacctgatga tcccggcaaa
     2401 gagtcttttc ggcgtaagcc ccgctaactg ttccggttag aaagacccga tcgcccgcct
     2461 tgaatccctc tgcccc
//
LOCUS       DASPRR010000436         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2354631, whole genome shotgun sequence.
ACCESSION   DASPRR010000436 DASPRR010000000
VERSION     DASPRR010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354631"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="VEL68_16020"
     CDS             complement(<1..1617)
                     /locus_tag="VEL68_16020"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ37529.1"
                     /translation="MAEKKSKTRTPRGGLFPDVMYVNGTILTMDEKNSRAEAVATMGD
                     KIVAVGKKAFIQKLAGPATHVVDLAGKTLVPGFNEPHNHFAMYGVYALRLVNLQSPPL
                     GPVSTMKSLIEVLREKAGKTPKGEWICGRGYDDTLMEEKRHPTRHDLDQASTNHPIYI
                     THVSGHLTVANSKALVLAGVTKDTVDPPGGVIRKDPKTSEPDGVLEEMPAQALVFRHL
                     PPLTMDLRLETMARSSRDYLRTGVASCSDAGVVFPGVGNPAEIIAYQRATRQGLVPIR
                     MTMMMGVGFLLGPDGKSPSFVTGFGDCLLKIGPAKIIVDGSIQGYTGWLAEPYHVPFK
                     GNSSYRGYPVTAPETLNRLVLAAHKAGCQIAAHGNGDAAIDAILDAYQLAQKESPRPD
                     SRHRIEHCQMAREDQLDRMAKLGVSPSFFASHTYYWGDRHKAIFMGPERAKRISPLKS
                     ALKRGIKFTLHSDCPVTPVSPLFCVFAAVNRITRNGEILGPEFRLTPEEALRAVTIDA
                     AWQTFDENIKGSIEVGKLADFTILAENPLAV"
     gene            1861..3474
                     /locus_tag="VEL68_16025"
     CDS             1861..3474
                     /locus_tag="VEL68_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37530.1"
                     /translation="MKAGKMSRNQLIAVPLMVFLFVFAFAPGQALAQADKTPRGGTLK
                     MIYQEPTHLNMAIVSGTPTGVPALQIFAGLVQFDENFKPQPYLAKKWEVSADGKNYTF
                     YLEEGATFHDGKPITSEDVAFSLETVKANHPFGVAMFRAVDRIDITDPHKVIFRLNHP
                     YPAFMAATHPLLLPVIPKHIYGPGEIRKNPANQKPVGSGPFKFVEWKRGQHIILERNE
                     NFFRKGKPYLDRIILEFITDSNARTVAMETGATHIIPFSYLSGDDALRLAKLPQFAMT
                     QKGYEAIGARTWFAVNLRKPPLNDKRVRKAIAHALDRNFIVKEIFRDFGKPATAPFHH
                     AGPFYNGKLPQYELNLNKANQLLDEAGFKRKTDGMRFSLNVDWIPTPTNQAVCEYTRE
                     QLKKVGINANLRASPDFPGWAAKISAWDFDLNFDSVFDYPDPVIGIERMYISKNIKNL
                     IWTNTMGYNNPEVDRLFAEAQVEQNFAKRKNLYHKVQEILMDELPLIWLQEGGFYTFY
                     SKEFDGIPMDVWGVLNPYDTIYWRKGRTG"
BASE COUNT          814 a          984 c          974 g          804 t
ORIGIN      
        1 cacggcaagg ggattttcgg ccaggatggt gaaatccgcc agctttccca cctcaatcga
       61 gccctttata ttttcatcga aggtctgcca ggcggcatca atggtgacgg ctcgaagggc
      121 ctcttcgggg gtcaggcgaa attccggccc caggatttca ccgttgcggg tgattcggtt
      181 gaccgcggca aagacgcaga acagggggga tacgggggtc accgggcaat cggaatgaag
      241 ggtgaatttg attccccgtt tcagcgcgga tttgagaggg ctgattcgct ttgcccgttc
      301 cgggcccatg aagatggctt tgtgccgatc cccccaatag taggtgtggg aggcgaagaa
      361 agaggggctt acgcccagtt tggccatccg gtccagctga tcttcccggg ccatctggca
      421 gtgttcgatc ctgtggcggg aatcgggcct gggagattct ttttgggcaa gttggtaggc
      481 atccaggatg gcgtcgatgg ccgcgtcgcc gtttccatgg gcggcgatct ggcaacctgc
      541 cttgtgcgct gccaggacca ggcggttcag tgtttcaggg gccgtgaccg gatagccccg
      601 gtaagaggaa tttcctttaa acggaacatg ataaggttcg gccagccatc cggtgtatcc
      661 ctggatggac ccgtcgacaa taatctttgc cggcccgatt tttagaaggc agtccccaaa
      721 tccggtgaca aaagatgggc tcttcccatc aggcccgagc aaaaatccca cccccatcat
      781 catggtcata cgaattggga ccaggccctg cctggttgcc ctctggtagg ctatgatctc
      841 ggccggattc cccaccccgg gaaagacgac cccggcatcg ctgcaggatg ccactccggt
      901 ccgcaggtaa tctcttgacg aacgggccat ggtctccagc cttaaatcca tggttaaagg
      961 ggggagatgt cgaaatacca aggcctgggc gggcatttct tccaaaactc cgtcgggctc
     1021 tgaagtcttc gggtccttcc ggatcacgcc gcccggagga tcgacggtgt ccttggtaac
     1081 tccggcaaga accagggctt tggagttggc cacggtcaaa tggcctgaaa catgggtgat
     1141 gtagatgggg tgattggttg aggcttgatc caaatcatgc cgggtaggat gccttttttc
     1201 ttccatgagg gtatcgtcgt accctcgacc gcaaatccat tctcccttcg gggttttgcc
     1261 ggctttttcc cggaggactt caatcaggct tttcatggtt gaaacagggc ccaggggcgg
     1321 gctctgcagg ttgaccaagc ggagggcgta gacgccgtac atggcaaaat ggttgtgagg
     1381 ctcgttaaaa ccgggaacca gggtcttccc cgcaaggtcc acaacatggg tcgcaggccc
     1441 agccaacttc tggatgaagg cctttttacc cacggcgaca attttatccc ccattgtagc
     1501 cacggcttca gcccgggagt tcttttcatc catggtcagg atggtcccat tcacgtacat
     1561 tacgtcggga aaaaggcccc cccggggggt ccttgttttt gatttctttt ccgccatccc
     1621 ttttctccaa tccgctcccg cttagggaag caaaggactt ttttatttca aaaaaatatt
     1681 ccttcctggt ataaatggtt gatctatgaa tcgtggggag agtagaatga tccggttgtc
     1741 ggacgcctta tccgaaccaa gtctattatc agcgagaaga atgagagatg caaagggaaa
     1801 atcaaatctc ctctttgatt ccttcaggga gaagatgaaa gtccaaagaa aggagcaaat
     1861 atgaaagctg gaaaaatgag tcgaaaccaa ctcattgcgg tccccctgat ggttttcctg
     1921 ttcgttttcg ccttcgcccc cgggcaggcc ctggcccagg cggataaaac cccccgcggg
     1981 gggactctga agatgatcta tcaggagccg acccacttaa acatggcaat cgtttccggc
     2041 actcccaccg gggtccccgc cctgcagatt ttcgccggcc tggttcagtt tgatgagaat
     2101 tttaaacccc aaccctacct ggcgaaaaaa tgggaagtat cggccgacgg gaaaaactat
     2161 accttttacc tcgaggaagg ggccacgttc cacgatggga agccgattac ctccgaagat
     2221 gtggcctttt ccctggaaac cgtgaaagcc aaccatcctt tcggcgtagc catgttccgg
     2281 gccgtggacc ggatcgacat taccgacccc cacaaggtca tctttcgact gaatcacccc
     2341 tatccggctt tcatggccgc gactcatccc cttctccttc cggtcattcc taagcatatt
     2401 tacgggccgg gagaaatccg caagaatccg gccaaccaga agccggttgg ttcaggacct
     2461 ttcaagttcg tcgagtggaa aagaggacag cacatcatcc tggagcgcaa tgagaacttc
     2521 ttccgcaaag gcaagcccta cctggaccgt atcattcttg aatttattac cgactccaac
     2581 gcccggaccg tggccatgga gaccggtgcc acacacatca ttcccttctc ctatctttcc
     2641 ggggatgacg ccctgaggct cgccaagctt ccccagtttg ccatgaccca gaaaggctat
     2701 gaggccatcg gcgccaggac ctggttcgca gtcaatctgc gcaagcctcc tctgaatgat
     2761 aaaagggtgc ggaaggcgat cgcccacgcc ctcgaccgga actttatcgt taaggaaatc
     2821 ttcagggact ttgggaagcc ggccaccgca cccttccacc acgccggccc tttctataat
     2881 ggaaaacttc cccagtatga gctcaacctg aacaaggcca atcagctcct ggacgaggcc
     2941 gggttcaagc ggaagacgga cggcatgcgg ttcagcctga acgtcgattg gatacccacc
     3001 cccaccaacc aggcggtctg cgaatacacc cgggaacagc tcaagaaggt gggaatcaac
     3061 gccaacctcc gggccagccc ggatttcccc ggatgggccg ccaaaatatc ggcctgggat
     3121 tttgacctca attttgacag tgtcttcgat tacccggacc cggtcattgg gatcgagagg
     3181 atgtatatct ccaagaacat caagaatctc atctggacca acaccatggg ttacaacaac
     3241 cccgaagtag atcgtctctt tgccgaggcc caggtagaac agaattttgc gaagcgtaaa
     3301 aacctatatc ataaggtgca ggagatttta atggatgaac tcccgctcat ctggcttcag
     3361 gagggcggtt tctacacctt ttatagcaag gaattcgatg ggattcccat ggatgtctgg
     3421 ggagttttga acccctacga cacgatttac tggcgcaaag gaagaacggg ttaagcctga
     3481 aagggattga agggaaaggg attcagtggc ttctagctcc ttcggctggt atctcctgag
     3541 gagaatcttg caggcgattc cggtcgtagt cggtgt
//
LOCUS       DASPRR010000437         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_107406, whole genome shotgun sequence.
ACCESSION   DASPRR010000437 DASPRR010000000
VERSION     DASPRR010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107406"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="lipB"
                     /locus_tag="VEL68_16030"
     CDS             <1..540
                     /gene="lipB"
                     /locus_tag="VEL68_16030"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXZ37531.1"
                     /translation="VITMGKSGKVQNVLAPQAIQEKGIKVIFTDRGGDVTYHGPGQMV
                     IYPILNLRLHGLSVPGYVWKLEETVIRLLDRFGIKAKRIEKLRGVWVENEKVAALGVH
                     LSRWISKHGLALNVNTNLDPFDLINPCGTGRRATSMARILGRELPMEEIETLIIQCFA
                     DVFGVSISQEPMEKLEAYL"
     gene            537..1391
                     /gene="lipA"
                     /locus_tag="VEL68_16035"
     CDS             537..1391
                     /gene="lipA"
                     /locus_tag="VEL68_16035"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXZ37532.1"
                     /translation="MKPEWLRRKLPAPEALEKMRSLLQRHGLHTVCQGALCPNQGECF
                     GQGTATFLILGKTCTRNCTFCAIPTEERPPAPDPDEPRRIAQAAAELGLKHVVITSVT
                     RDDLVDGGASLFARTVEALRKMNPQITVEVLIPDFQGSVEALRKVTDSAPNILNHNLE
                     TVSRLYPEVRPQAVYPRSIELLKRAKAMAPEEFTKSGLMLGLGEEKEEILGVMADLRE
                     VSCDFLTLGQYLQPSGRHHPVIRFVSPEEFEELRVEGMKMGFRAVFSAPLVRSSFHAA
                     EVFEKISQ"
     gene            1484..2242
                     /locus_tag="VEL68_16040"
     CDS             1484..2242
                     /locus_tag="VEL68_16040"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXZ37533.1"
                     /translation="MRFQGKVVLITGAGVGIGRAAAIRFAKEGAQVAVNSLTPANGKE
                     TLRLLMEAGGQGIYIQGDVSRDADARRMVDETVKAYGRLNILVNNAGIVIPGRVDNMS
                     EEDWDKSMAVNLKGVFLVSKYAIHEMRKIGGGVIVHNASTVAIKGVKDRGAYTASKAG
                     VWALTKAMAADYLSENIRVNCICPGTTYTPSLERRIQAFPDPEKARADFIARQPMGRL
                     GKDEEIAAAILFAASDEVAFMNGATIAIDGGMTI"
     gene            2314..3477
                     /locus_tag="VEL68_16045"
     CDS             2314..3477
                     /locus_tag="VEL68_16045"
                     /inference="COORDINATES: protein motif:HMM:NF016083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HXZ37534.1"
                     /translation="MNLVYVLLAGALVVCLATGKDIFQTAGIFAQGIFDPSTLQLMGV
                     ITLIEMLTIFLQLTGSLQRILNALRKVFADSRVLIALVPTLLGLFPVPGGAMLSAPMV
                     GVYGDEIGFKPNRKSAINLFFRHIWDQVFPFKPHLILGAAVLNIPLFTLIGWHLPVTL
                     LSALVGYWYLIGRHSKPEAVSDSQEDSAGGRALWVEVMPLLLPLILGLGFKIDFVYAT
                     AIGLLFGFLTQAVSRDVLGKVFSQGFRIQLLLLIAAVMIFKVALESSGLLKTLSQALP
                     GYGIPLVFLALALPLIISTATGMELVAVGMAYPMLVGLVPEGSPALPYILIMMTANAI
                     GQTHSPVHICMVVGNEYFGAQLGKVVKMSIIPQGFRLMATFFFAWLLYLYLPR"
     gene            3560..>3665
                     /locus_tag="VEL68_16050"
     CDS             3560..>3665
                     /locus_tag="VEL68_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin deacetylase"
                     /protein_id="HXZ37535.1"
                     /translation="MEGMARDFIGYGGNPPKVEWPGGARIALNLVINYE"
BASE COUNT          789 a         1022 c         1039 g          815 t
ORIGIN      
        1 gtgattacga tgggcaagtc gggtaaggtc cagaacgtcc tcgcccccca ggccatccag
       61 gaaaagggaa tcaaggtcat ctttacggac cgtggaggag atgtgaccta ccacggaccc
      121 ggacagatgg tcatctatcc aattttaaac ctacgcctcc atggcctcag cgtacccggt
      181 tatgtttgga aactggagga gaccgtgatc cgtctccttg accggttcgg gataaaagcc
      241 aagcggatcg aaaagctgag gggggtctgg gtggagaatg aaaaagttgc cgccctggga
      301 gtccatttat cccgatggat cagcaagcac ggcctagcct taaacgtgaa taccaacctg
      361 gacccctttg acctcatcaa tccttgcggg accggacgga gggcgacctc catggccaga
      421 atcttgggcc gggagcttcc catggaggag atcgagaccc tgatcatcca atgcttcgcc
      481 gatgtcttcg gagtttccat ctctcaggag ccgatggaaa agctggaggc ctacctatga
      541 agccggaatg gctgaggaga aagctgccag ccccggaagc tctggaaaaa atgcgaagcc
      601 tgctccagcg tcacggactc cataccgtct gccagggagc cctttgtccc aaccaggggg
      661 aatgcttcgg ccagggcacg gccactttcc tgattctggg aaagacctgc acccggaact
      721 gcaccttctg cgccatcccc actgaagagc gccctccggc gccggaccca gacgaacctc
      781 ggagaattgc tcaggctgct gctgaactcg ggctaaaaca cgtggtcatt acctcggtga
      841 cccgggatga tcttgtagac ggcggggcct ctctctttgc ccgtactgtc gaggccttaa
      901 ggaaaatgaa cccgcaaatc accgtggagg ttttaatccc ggattttcag gggtcggtgg
      961 aagctctcag gaaggtcacg gacagcgccc ccaatatcct caatcacaac ctggaaacgg
     1021 tctcccggct ttaccctgaa gtccggccac aggcggtgta tccccgctcc atcgagcttt
     1081 tgaaaagggc caaggccatg gctccggagg aattcacgaa gtcaggcctc atgctcggtt
     1141 tgggagaaga aaaggaagag attttggggg tgatggccga tctgcgggaa gtatcctgcg
     1201 acttcctaac attaggtcag tatctccagc cctctggaag gcatcatccg gtgatccggt
     1261 ttgtctctcc ggaggaattt gaagagctgc gggtcgaagg gatgaagatg ggattccggg
     1321 cagtcttctc cgcccccctg gtccgcagtt ccttccatgc cgccgaagtc tttgagaaaa
     1381 tcagccaata gctttcagcg gtcagcaatc agcgaagaac gaagacgatg cattggctgg
     1441 ttgctgaatg ctgatcgctg atcgctttta gggggaggtt ttcatgcggt ttcaggggaa
     1501 agtggtattg attaccggag ccggagtcgg gatcggccgc gccgccgcca ttcgcttcgc
     1561 aaaggaagga gcccaagtcg cggtgaattc actcactccg gctaacggca aggagacgtt
     1621 gcgccttcta atggaggccg gaggccaggg aatttatatt caaggggatg tttccaggga
     1681 tgccgatgcc cgacggatgg ttgatgagac ggtgaaagcc tacggccggc tgaacatcct
     1741 ggtgaacaac gccgggatcg tcattcccgg aagggtggac aacatgtctg aggaagactg
     1801 ggacaagagc atggcggtga acctgaaagg agtcttcctg gtctccaaat acgccatcca
     1861 cgaaatgcga aagatcgggg gcggggtgat cgtccacaac gcctccactg tggccatcaa
     1921 gggagtgaaa gaccggggag cctacactgc ctccaaagcc ggagtttggg ccttgaccaa
     1981 agccatggcc gccgattatc tctcggaaaa tatccgggtg aactgcatct gccccggtac
     2041 cacttatacc ccttccctgg aaagaaggat ccaggccttc cccgatcccg agaaagcaag
     2101 ggcggatttc atcgcccgcc agcccatggg gcggctggga aaggatgaag aaatcgccgc
     2161 cgccattctc tttgctgcat cggatgaggt cgctttcatg aacggggcca ccatcgccat
     2221 cgatgggggg atgaccattt agttcacccg caggttcgcg cagattttag cagaaaagga
     2281 ctgactttag ggggaaatcc atattcgcaa gctatgaatc tagtctatgt attgctcgcc
     2341 ggagccctgg ttgtctgttt ggccaccggg aaggatattt ttcaaaccgc aggaatcttc
     2401 gcccagggaa ttttcgatcc ttccacgctt cagctgatgg gggtgattac tctcattgag
     2461 atgctcacca tcttccttca gcttaccgga agccttcaga gaatcctcaa cgctctgcga
     2521 aaggtttttg ccgactcccg ggtgctgatc gccctggtcc ccaccctttt gggtctattt
     2581 ccggtcccgg ggggagctat gctctccgct ccgatggtag gagtctatgg agatgagatc
     2641 ggcttcaagc ccaatcgaaa atcggccatc aacttattct tccgccatat ctgggaccag
     2701 gtcttcccct tcaagccgca tctgatcctg ggggccgctg tgctcaacat cccccttttc
     2761 acccttatcg gttggcatct tcccgttacc ctgctgagcg ctctcgtggg gtactggtat
     2821 ttgatcggcc gtcactcgaa accggaagcc gtttctgact cccaggaaga ctctgccgga
     2881 gggcgggccc tctgggtcga agtcatgccc cttcttctcc cgctcatcct ggggctggga
     2941 ttcaagatcg actttgttta cgccacggcc attggactgc tgttcggttt tctcacccag
     3001 gccgtctcca gggatgtcct cgggaaggtc ttcagtcagg gattccgaat ccaactcctt
     3061 ttgcttatcg ccgcggtgat gatctttaaa gtggccctgg agagttcggg cttgctgaaa
     3121 accctctccc aggcgctccc cggatacgga atcccgctgg tttttctggc ccttgctctg
     3181 cccctcatca tcagcacggc taccggcatg gaactggtgg cagtggggat ggcttacccc
     3241 atgctggtgg gattggtccc cgaagggtcc cccgcccttc cctatatcct gatcatgatg
     3301 actgccaacg ccatcgggca gacccattcg cctgttcata tctgtatggt cgtgggaaac
     3361 gaatatttcg gggctcagct gggaaaggtc gtgaagatga gcatcattcc ccaaggcttt
     3421 cggctgatgg ccactttttt cttcgcttgg cttttgtatt tgtacttgcc ccggtgaacg
     3481 gaaagaccta tttcctaaac ctaagccggc ccagccggta tcaagcaaga cagtgcttat
     3541 ccccaggagg gaacagaaaa tggaaggaat ggcacgtgac tttatcggct acggggggaa
     3601 tcccccaaaa gtggaatggc cggggggagc ccgtatcgcc ctgaatctgg tcatcaacta
     3661 cgagg
//
LOCUS       DASPRR010000438         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1069194, whole genome shotgun sequence.
ACCESSION   DASPRR010000438 DASPRR010000000
VERSION     DASPRR010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069194"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1075
                     /locus_tag="VEL68_16055"
     CDS             41..1075
                     /locus_tag="VEL68_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HXZ37536.1"
                     /translation="MAGLFDSLKIQEIELRNRIAFAPVVTNFGLRNEQTTRYYAERAR
                     GGAGLIIVHGTPVDLFLKLGWVQRLKPLIEAIQREGARVVIQLWHGNDLKGEPVAPSV
                     QGPCRAITREEIRVVVEKFAAAARHCREAGFDGAEVHGAHGYFINQFFSPLANQRQDD
                     YGGTPQKRMRLAAELVEGMRKAVGRGFLLLYRHSAVDGEPGGTSIEESIQLGKTLETR
                     GLDILDVSAGRGQKDNLSIPESSAPEGTHADLAGRIKAAVSIPIIAVGRIQRRAVADR
                     ILLEGKADLIALGRQLLADPFWPEKLRQGKDEDVVLCTYCDTCTREMRNGKPIFCPQN
                     PHLGKEAETG"
     gene            complement(1166..1627)
                     /locus_tag="VEL68_16060"
     CDS             complement(1166..1627)
                     /locus_tag="VEL68_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXZ37537.1"
                     /translation="MKDRLLSAVILFLFLLFSWAEEEIIYSQQREEGLFQLTIKEKKI
                     QVEVVRTEGEKARGLMFRDKLGADEGMLFVYEGEEFLTFWMKNTPLPLSIAFMDQKGR
                     IVDIQDMEPFSLRTHTSARPARYALEMNRNWFQKNGIKVGDLAKMPPSFEK"
     gene            1793..1966
                     /locus_tag="VEL68_16065"
     CDS             1793..1966
                     /locus_tag="VEL68_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37538.1"
                     /translation="MRTLLRRLRVILPVMGLVAGCGERPGSEGAKVFLALGVCLAAVF
                     LIFYIVIKSREKD"
     gene            2032..2520
                     /locus_tag="VEL68_16070"
     CDS             2032..2520
                     /locus_tag="VEL68_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXZ37539.1"
                     /translation="MKLNRVLTATDFSKQSGFALEWAAYLAQCMKADLILLHVISEEE
                     GKIIEEVIGEGAVVQIPKGIRQNVVEDRQKKMREQYDMVVSREIKASLKMEELIRIGV
                     PFLEIIRAAKEKDVDLIVLGTHGRSGLSHVLIGSVAEKVVHHAHCGVLTVKHPQYKFV
                     AP"
     gene            2537..3013
                     /locus_tag="VEL68_16075"
     CDS             2537..3013
                     /locus_tag="VEL68_16075"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXZ37540.1"
                     /translation="MNRKYPDHPIVGVGGIIFKGDQVLLIKRGKEPGLGQWSIPGGVV
                     HTGETLKEAVVREVFEETHLEVEVLALAKILERIFRDPDGRIAYHYVLVDFLCQVVKG
                     TLQPDSDAQEACFVSLNELASYKVVPITLEVIRRADWLRKNPETEILPPGVHGVYD"
     gene            3006..>3335
                     /locus_tag="VEL68_16080"
     CDS             3006..>3335
                     /locus_tag="VEL68_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37541.1"
                     /translation="MTKLPGKIGKKVSALKDTRSPIFWASLAVIFSVIICLLVFWGGG
                     KSQKPGRIPAEVLSRIDQEKAQAQQEFADFIQTPAGKLWQKHPYWSPEICRKIVAGEI
                     VAGMSKEH"
BASE COUNT          816 a          840 c          966 g          713 t
ORIGIN      
        1 tttggaatct ggttttagcg cttctctcta gaaagggatt atggcgggtc tcttcgattc
       61 tctaaaaatt caggaaattg aactccgcaa ccggatcgcc tttgctccgg tggtcacgaa
      121 tttcggcctt cgtaacgagc agacgacgag gtattatgcg gaacgcgccc ggggaggggc
      181 gggtttgatc atcgtccatg gaacgccggt ggaccttttt ctcaaattag gttgggtcca
      241 aaggctgaaa cctctgattg aagccataca acgagaaggg gcccgggttg tcattcagct
      301 ctggcatgga aacgatttga aaggggaacc ggtggcccct tcggtccagg ggccctgccg
      361 ggcaatcacc cgggaggaga tccgggtggt ggttgagaaa tttgccgcag ccgcccgtca
      421 ttgccgggag gcgggttttg acggggcgga ggtccacggc gcccatgggt attttatcaa
      481 ccagtttttc tcccccctgg ccaatcagcg ccaggatgat tatgggggaa ccccccagaa
      541 gcggatgcgg ttagcggcgg aactcgtcga gggcatgcgg aaggccgtgg gccggggctt
      601 ccttctgctg taccggcaca gcgcggtgga tggagaaccg gggggcacga gcatcgaaga
      661 gagcattcaa ctggggaaaa cccttgagac ccgcggactg gatatccttg atgtttcagc
      721 cggcagggga caaaaagaca acctttcgat ccccgaatct tccgctccgg aaggaaccca
      781 tgccgatctg gcgggtcgga taaaggcggc cgtttccatc ccgattattg ccgttgggcg
      841 aattcaaaga cgggctgtgg cagatcggat tctgctggaa ggaaaggccg acctgattgc
      901 cctgggacga cagctcctgg ctgacccctt ctggccggaa aagttgcggc agggaaagga
      961 cgaagatgtc gtcctttgca cctattgcga tacttgcacc cgcgagatga ggaacggaaa
     1021 gccgatcttc tgcccccaga atccccatct gggaaaagag gcggaaacag gatagaagcc
     1081 aaaaggagaa ggcgaagggg cgaggggacg aaatgggcaa aaagagaaaa gcaataacga
     1141 accatgagga acgagcaacc agcaactact tttcgaagga agggggcatt ttagccaagt
     1201 ctcccacttt gatcccattc ttttgaaacc aatttctatt catctccagc gcatacctgg
     1261 caggacgggc ggaggtgtgg gtccgcaggc taaagggctc catgtcctga atgtccacga
     1321 tccttccttt ttgatccatg aaggcgatgg acaagggaag aggggtattt ttcatccaga
     1381 aggtgaggaa ttcctctcct tcgtagacga agagcatgcc ttcgtctgcc cccaatttat
     1441 ccctgaacat caagccccga gccttttcgc cttctgtgcg gaccacctca acctggattt
     1501 tcttttcttt gatggtgagc tggaacaagc cctcttcccg ctgctgggaa taaattatct
     1561 cttcctccgc ccaggagaaa agaaggaaaa gaaaaagaat aactgcggaa agtaagcgat
     1621 ctttcatcga atgttctcca cccgccgatc ctgccttcaa accacagcct tgcccccggg
     1681 ccgcaagaaa aggtgtcaca aaactgaagc tgggcggggc tttaaaactg cttgacagcc
     1741 caggtccaat ataattaaaa tccgccataa actcaatggg atttcagagg atatgagaac
     1801 cctcctaaga aggttgcggg tgattctgcc ggtcatggga ctcgtcgccg gatgcgggga
     1861 acgaccgggg agcgaagggg ccaaagtctt tctggccctg ggggtctgcc tggctgctgt
     1921 atttctcatt ttttatattg tgatcaaaag ccgggaaaaa gattaggatt gcggaacggg
     1981 gggctctgtg gttagatttt tgtagaggga ctattttctt aaggaggggc tatgaaattg
     2041 aatcgggttt tgaccgcaac ggatttttct aaacagtccg ggtttgccct ggagtgggca
     2101 gcctatctgg cccagtgcat gaaagccgac cttatccttc tccatgtaat cagcgaagag
     2161 gaaggcaaaa tcatcgaaga ggtcatcggg gaaggcgcgg tagttcaaat ccccaagggc
     2221 atccggcaga acgtcgtcga agatcgccaa aaaaagatga gagagcagta cgacatggtg
     2281 gtttccaggg agatcaaggc ctccttaaaa atggaagaat tgatccgcat cggggttccg
     2341 ttcctcgaaa ttatccgcgc cgccaaagaa aaggacgtgg acctgattgt cctgggaacc
     2401 catgggcgca gcggcctttc ccacgtgctg atcggaagcg tggccgaaaa ggtcgtgcac
     2461 catgcccatt gcggggtcct gaccgtaaaa cacccccaat acaaattcgt cgccccttaa
     2521 acctccggct cccggcatga atcgcaagta tccggatcat ccgatcgttg gagtaggggg
     2581 aatcatcttc aagggtgatc aggtcctcct gatcaagcga ggaaaggaac cgggcctcgg
     2641 acaatggtcg atcccgggag gagtagtcca cacgggagag acgttgaaag aggccgtggt
     2701 ccgagaggtc tttgaggaga ctcatctgga ggtggaggtt ctggccttgg cgaaaatcct
     2761 ggagcgaatc ttccgcgacc ccgacggccg aatcgcctac cattatgtat tagtcgattt
     2821 tctctgccag gtggtgaagg gcaccctgca gccggattcc gatgcccagg aggcatgttt
     2881 tgtctccctg aatgaactgg cctcttacaa agtcgtcccc atcaccctcg aagtgatccg
     2941 ccgagccgac tggttgcgga agaacccaga aacagaaatc ctgccacccg gggtccatgg
     3001 cgtgtatgac taaactccca gggaaaattg ggaagaaggt ctcggccctc aaggataccc
     3061 gctctcccat cttttgggcc tccctggccg taattttctc ggtcattatt tgtttgctgg
     3121 tattttgggg cggaggaaag agccaaaaac cgggacgaat tcctgccgaa gtgctttcca
     3181 ggatcgatca agaaaaagcc caagcccagc aagagtttgc cgatttcatt cagacaccgg
     3241 caggaaaatt gtggcaaaag catccttact ggagtcccga aatatgccgg aaaatcgttg
     3301 cgggggaaat cgttgccgga atgagcaagg agcac
//
LOCUS       DASPRR010000439         9507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_913965, whole genome shotgun sequence.
ACCESSION   DASPRR010000439 DASPRR010000000
VERSION     DASPRR010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913965"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..1279
                     /locus_tag="VEL68_16085"
     CDS             455..1279
                     /locus_tag="VEL68_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HXZ37542.1"
                     /translation="MEKPFEEYFIQEKPYYIPIRDEEEIFTSAYKARLPVLLKGPTGC
                     GKTRFVEYMTYMLGREMGIPLPLITVACHEDLTGSDLVGRYLLKGEETIWVDGPLARA
                     VKNGAICYLDEIVEARKDTTVLIHPLADHRRILPVEKRGQILEAHENFLLVISYNPGY
                     QSVLKNLKHSTRQRFVAIDFDYPPAELEAKIIAHESGVNFEAAASLAKLAEKVRNLKE
                     HGLEEGVSTRLLIYAGQLMVQGVAPRRACEVAVAQAVTDDLDLARGIVEVIGAIFP"
     gene            1441..4134
                     /locus_tag="VEL68_16090"
     CDS             1441..4134
                     /locus_tag="VEL68_16090"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXZ37543.1"
                     /translation="MNEEEKQKWNEFAQELRRRVRWYMDKVDGALGTALEALPCLKRQ
                     EREDLLKIGWVVAERSSMLAVIFLRIGPKILGSLPLDIRPVVIRWAAILADHSRETLI
                     DFLERSQEVLAILPEEGSSLLLSQGLQLAGLDPSISYKFFLNLPKIGKEISRDRLSSW
                     FEEGLTLIPQRIPAAMAYFGLESRRSQDRVLEEQSSVALEEVSRPMKLFAQALTGRSL
                     ALRPIQELRNGIQNPAGPLPCTDGETIYLPGMVKNFPTREINSLAFKLATAHQAGYVE
                     FGTFKFRLSAIQDLFPSEFMAACLRGISEKGKEISPLEAFFHLFPRKELARDLFHVLE
                     GARVDHCLQREYQGLKKEMNRFLPLVMEARPAISSLPLQQAVLESILRWALLGEPMKP
                     RFLALLSQGDDLTPHLMALTRPGAGVRNSARAAVLLYRRLSRIPNRKPACGWEGEVLP
                     ALSLPLSPVGGMDPASRLVPGEEPYQNLAPLSHWGELRPELVQKKLRLREVQNLLNQM
                     EMGVPLSPEVLQALLESGMELEVSEGLGGDEFQGLFITDLKGLKKQMGMQEASQRAKE
                     RLEGEMRSLLSEVAEETGEDQHYYDEWDCQIADYRVRWCRLKEKDAEIGSTEFVDRTL
                     DSYSDLVTEVRRQFQMLKPERFKKIPNLERGEEIDLNAAVEASVDRRAGQSPSEKIYI
                     EKNRKDRDFSTLFLLDMSASTDERVDGKDRKPENPPSEKEKKIIDIEKEALVVMAEAL
                     KEIGDEYAIYGFSGYGRKEVDFYAIKDFQEEYGDRVKGRIEKIKPQRSTRMGTAIRHA
                     AEKMAGRESRIKNLILISDGYPQDYDYGDDRSNKEYALQDTLIALEEAARKQIHTFCI
                     TVDRAGHDYLRKMMPPSRYLIIEETAALPRELPKIYRKLTT"
     gene            4305..5279
                     /locus_tag="VEL68_16095"
     CDS             4305..5279
                     /locus_tag="VEL68_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXZ37544.1"
                     /translation="MLKTRITEMLGIQYPIIQGGMAWLGLAELVAAVSNAGGLGIIGS
                     VTFPTPEELRNEIRRTRRLTDKPFGVNITLLPAMRQLPNDGFVQVIVEEGISVVETTA
                     GRPEKYVDILKRANVKVMHKVGSVRHAKNAEKIGCDAVIAVGFEGAGHPLMDDVSLWN
                     LVPRMVDTVTIPVIAAGGTTDHRQLMAALALGAEAVLMGTVFMAAKESVAHPKFKQAL
                     INATECDTVLIQRSIKNQTRVFRNRTADEVLNLESRGASLEELMPMISGQRGKNALML
                     GDIDEGTMTCGQGVGLIKNELTVKEVIDGMVKGAETLLNRLNSLNRSQ"
     gene            5304..6458
                     /locus_tag="VEL68_16100"
     CDS             5304..6458
                     /locus_tag="VEL68_16100"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37545.1"
                     /translation="MKTKKGIPVIFCAWVIFFIFQSPAAGENVINIGFTGPLTGPAAA
                     IGIDSLHGNMIAAKEINDEGGVAVAGKKYKVTIQSYDDEGVAAKAVAGMQRLKDKYEV
                     PVINQNISGSLMAMLERNEKMGILLIGFFKIPDATKRGNKLILRHQQTIDEDAQGLAK
                     ETVRVLKAKTYAMISDVGDYGKTSVKAYQQAFEKLGVKIVANEWLDGRTQTDFRGQLT
                     KIKAANPDVIMVTAYDEATAGVIKQAHELGIKTPFAVSTGFAAAGEKLTGPDLIEGYL
                     RRVEFTNSRPMPPGVARYRTQLYPAMGYKEPIGGFGVNCYASVHIIVRAMQKAGTTTD
                     AFKIRQAVPLVVPLEEKYDSTGLKAFAEDGEGIMSAQIGRYQKGIVVPVK"
     gene            6612..7472
                     /locus_tag="VEL68_16105"
     CDS             6612..7472
                     /locus_tag="VEL68_16105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37546.1"
                     /translation="MAEFLQYTVNGLIAGSTYGLLALAMTLIYGILAIPDFSLGAIYA
                     LGAFISFYIIQWLGPEYYLFSLFPIIILAGLLGTCNERIVFKPLEKAPHAAGFIAALG
                     LYSILEGGWNVWFGSDWRNINSPYNDMAIALGPISLTVQRLILFAVCLLFALGTFLLI
                     FRTLTGKKIRAFSENREVAQLMGVNTRRVSALTFFIGYALAGIGGALVAPTALVGASM
                     GLTPIIKSFIVVALGGLGSVTGAIVGGFILGLGENFGAAYISSMYKDLFSFGLFLGVL
                     ILMPQGLYRR"
     gene            7566..8516
                     /locus_tag="VEL68_16110"
     CDS             7566..8516
                     /locus_tag="VEL68_16110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37547.1"
                     /translation="MRILTYLFLGLLALLLPVVIRSNYWLYMFTLVGIYIILVASLDL
                     IYGYTGLVSLGHAAFFGIGAYACGILQTKLGFSFLPALLLGAFFTCIASAVLGWPMLR
                     IKGPYFALGTLALGLMVTIVIHNWDSLTGGVSLSGIPLPPVIQLLGYTVDFSSKRTYY
                     YFVLLFAFLALFFIRQITHSRPGRAFQSIRENADLAEALGVDILRYKLIAYVMASTIA
                     GVAGGLYASYMGSIEPAIAGGHMSFHLLVMIVIGGARTITGEIVGPLLLWFVPEFLEA
                     TQVYRPLFFGFILLVVIIFMPMGIAGKLRSLHPRIAQWIP"
     gene            8504..9295
                     /locus_tag="VEL68_16115"
     CDS             8504..9295
                     /locus_tag="VEL68_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37548.1"
                     /translation="MDSLNLESRAPLLRVEKLSVSFGRLLAVHDLAFQIHSGEIFGLI
                     GPNGAGKTTVFNAITGTVPLSEGKIRFSETDITGLKTHQITTRGVVRAYQAATIFPQT
                     PVGEHVMTGLHCRTLTSVWGAFLHTPFSRKEEGQNRKKVKELLEFSNLADFREQPASS
                     LTWAQQKRLMLVTALATIPKLILLDEPVAGMNASEIQEMIELIATVRKEGVTVFIIEH
                     NMKVMMQICDRILVMNYGQKLSEGIPKEVARDPKVIEAYLGYRQN"
     gene            9292..>9507
                     /locus_tag="VEL68_16120"
     CDS             9292..>9507
                     /locus_tag="VEL68_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ37549.1"
                     /translation="MIRLRQIDVFYGKSQALHGVSLSVGAGEIVALIGANGAGKTTVL
                     KAISGLTPCTAGEIIFKEKSLKGLKPEE"
BASE COUNT         2218 a         2407 c         2691 g         2191 t
ORIGIN      
        1 aagatcctgg tggacaatcc agcgcggctt tacgggtttt aaactgctgc gggccgccac
       61 gcaggtggaa ccaagtcatg agggaagggg ggcacgttgc tcggaagctc aaaaatattg
      121 atttaccctc cgagccagga aatgcggttt aggtctctag gcaacgccct ctcttcccag
      181 cagtgtgccg aagatcgtgt cgtttctgag gcgacaagct gatcaaacac gatctcgccc
      241 agcagtctgt tcataaactc tttccaaaac ctgaaccggg ccaaaatttc gataccctag
      301 tctgattttg ccggcctaag ggaaaggtta cctctataat cagctagccg taatcttaat
      361 tccgcaacct gccatctgga atcggatatc gccctgccca atttccttgc cattctttcc
      421 acggatttgc taatcttgaa acatgaacag aaaaatggaa aaaccatttg aagagtattt
      481 catccaggag aagccttatt acattcctat ccgggatgag gaggaaatct tcacctcggc
      541 ttacaaagcc cgacttccag tcttgctcaa aggcccgacc ggatgcggga agacccggtt
      601 tgtggagtac atgacctaca tgctgggcag ggaaatgggg attccccttc ccttgatcac
      661 cgtggcctgc catgaagacc tgaccgggtc tgacttggtc ggaaggtacc tcttgaaggg
      721 ggaggagacc atctgggtcg atgggccgct ggcccgggcg gtgaagaacg gcgccatctg
      781 ctacctcgat gaaatcgtgg aggcccgcaa agataccacc gtcctcattc atcctctggc
      841 cgaccaccgg cgaatccttc cggtggaaaa gagaggacaa atcctggagg ctcatgaaaa
      901 tttcctcctt gtgatttcct acaatccagg ctaccaaagc gtcctaaaaa acctgaagca
      961 cagcacccgg cagcggtttg tggctattga tttcgattat ccccccgcag agctggaggc
     1021 caagatcatc gcccacgaaa gtggggtcaa cttcgaggcg gccgcttccc tggccaagct
     1081 ggcggagaaa gtccgcaacc tcaaagaaca cgggctggag gagggggttt ccacgcgcct
     1141 cctgatctac gccgggcagc ttatggtcca gggggtcgcc ccccggagag cctgcgaagt
     1201 ggccgtggcc caggcggtga ccgacgactt agacctggcg cggggaatcg tggaagtcat
     1261 cggtgctatt ttcccgtaaa gacattttta ccatagagac acagagaaca cggaggaaaa
     1321 aattagaaat atataggggc aaaaaaagta ttaggtttca aaacccttgc ccctatgctt
     1381 tgattttagg cctattataa ttctctgtgc gctctgtgcc tctgtggtga atatcgaatt
     1441 atgaatgaag aagaaaaaca gaaatggaat gaatttgccc aggagctgag gcggcgggtc
     1501 cgctggtaca tggacaaggt cgatggggcc ctgggaaccg cgctggaggc tcttccttgc
     1561 ttgaagagac aggagagaga agacctatta aagatcggat gggtagtggc tgaaaggtcc
     1621 agcatgctgg cggtaatctt cctgcggatc ggcccaaaaa ttctgggttc cctccccctg
     1681 gacatccggc cggtggtaat ccgatgggct gcgatcctgg ctgaccattc ccgcgagacg
     1741 ctcatcgatt ttttggaaag gagtcaggag gtcctggcga tccttccgga ggaaggaagt
     1801 tcccttcttc tttctcaagg gttgcaactg gccggcctgg acccgtcgat ttcctacaaa
     1861 ttctttctca acctccccaa gatcggtaaa gaaatttcaa gggaccgcct ctcctcctgg
     1921 ttcgaagaag gacttaccct tatcccccaa aggattcctg cggccatggc ctatttcggc
     1981 ctggagtcca ggcggtccca ggatcgggtt ctggaagaac aatcctccgt cgctctggaa
     2041 gaagtctccc gccccatgaa gctcttcgcc caagccctca ccggccggag cctggctctg
     2101 cggcccattc aggaattaag aaatgggatt caaaatccgg ccgggcccct accatgtacg
     2161 gatggggaga ccatttattt acctggaatg gttaagaatt tccccacccg ggagataaac
     2221 tccctggctt tcaagttggc aactgcccat caagccggtt acgtggaatt cgggaccttc
     2281 aaattccgtc tctccgccat tcaggacctt ttcccttcgg agtttatggc ggcctgcctt
     2341 cgggggattt cggaaaaagg aaaagagatt tctcccctcg aggctttttt ccatctcttc
     2401 ccgcgaaaag agcttgctcg ggaccttttc catgtcctcg aaggagcaag ggtggatcac
     2461 tgccttcagc gggaatacca ggggttgaag aaagagatga atcggttcct ccccctggtc
     2521 atggaggccc ggccggccat ttcctcacta cccttacagc aggcggttct ggaatccatt
     2581 ctccgctggg cgctcctcgg agagcccatg aaacctcgat ttctagctct tctttcccaa
     2641 ggggacgatt taaccccgca ccttatggct cttacccggc cgggggcagg cgtcaggaat
     2701 tccgcccggg cagcggtcct cctgtaccga aggttgagcc ggattcccaa cagaaagcct
     2761 gcctgcggtt gggagggaga ggtgctgccc gcgttaagcc tgccgctttc tccagtgggc
     2821 ggaatggacc cggcttcccg ccttgtcccg ggcgaagagc cctaccagaa tctcgcgccg
     2881 ctttcccatt ggggagagct gcggcccgag ctggtgcaga agaagcttcg cctgcgggaa
     2941 gtccaaaacc tgctcaatca gatggaaatg ggagttcccc tgtccccgga ggttttacag
     3001 gctctcctgg aaagcggaat ggagctggaa gtatccgagg gattgggggg ggatgaattc
     3061 caggggctct tcatcaccga tttgaaaggg ttgaaaaagc agatggggat gcaggaggcc
     3121 agccagcgag caaaagaaag gctcgagggg gagatgcgtt ctctcctgag tgaagtggcc
     3181 gaggaaacgg gggaggatca gcactattac gacgaatggg attgccagat cgcggattac
     3241 cgcgtgaggt ggtgccgcct gaaggagaaa gatgcggaaa tcggctctac ggaatttgtt
     3301 gatcggaccc tggactccta ttccgatctc gtaactgagg tgagacggca gttccagatg
     3361 ctcaagccgg agcggtttaa aaagattcca aacctggagc ggggggaaga gatcgacctc
     3421 aatgcggccg tggaggcttc cgtggaccgg agggcgggcc aatccccctc ggaaaagatc
     3481 tacatcgaaa aaaatcggaa ggaccgggat ttctccaccc tttttctgct ggatatgagt
     3541 gcttccacgg atgagcgggt ggacgggaag gatcgaaagc cggaaaatcc cccttcggaa
     3601 aaggagaaaa agataatcga catcgaaaag gaagccctgg tggtgatggc ggaggccctg
     3661 aaagagattg gggacgaata cgccatttac gggttttctg ggtacgggcg gaaagaggta
     3721 gacttttacg ccatcaaaga tttccaggaa gaatacgggg accgggtaaa aggccggatc
     3781 gagaaaatca aaccccagcg ctccacgcgg atggggacgg ccatccgcca cgcggcagaa
     3841 aaaatggccg gccgggagtc caggatcaag aacttgatcc tgatcagcga tgggtatcca
     3901 caggattatg attacgggga cgatcgctcc aacaaagaat atgccctcca ggacaccctg
     3961 attgccttgg aggaggcggc ccgcaagcag atccatacct tttgcatcac cgtggaccgg
     4021 gccgggcatg attaccttcg caaaatgatg cctccctccc gctatctgat catcgaagaa
     4081 accgcggccc tgccgcggga gctgcccaag atttaccgga agctgaccac gtgaagaaaa
     4141 aagcgtcgtc tctcagtagg gcgattgaag ctaaggatca cggcctttcc tatcattcgt
     4201 attggcattg ataagaaatc gagagacccc attatgagct tcctaaacga catccgcttt
     4261 gagttttccg ggcctattat agaactacat tcgggaggga aaacgtgctc aagaccagaa
     4321 ttacagagat gctgggcatt cagtatccaa ttattcaagg tggaatggct tggctcggtc
     4381 tggcagaatt agttgcagcg gtctctaatg ccggaggttt gggtatcatt ggttctgtga
     4441 ctttccccac gcccgaggaa ctgcggaatg agatacgcag gacaaggagg ctcaccgata
     4501 aaccattcgg ggtcaatatc actttgctgc ctgcaatgag gcagttgccc aacgatggtt
     4561 ttgttcaggt cattgtggag gaggggattt ccgtagtgga aacaaccgct ggtagaccgg
     4621 aaaagtacgt ggatattctt aagcgggcca atgtaaaagt catgcataag gtggggtctg
     4681 tgaggcatgc caaaaatgcg gagaaaatag gctgcgatgc ggtcattgcc gtgggttttg
     4741 agggggccgg acatccttta atggacgatg tctcgctgtg gaatctcgtc ccgagaatgg
     4801 ttgacacagt caccatcccg gtcatcgcag ccggtgggac aaccgaccat cgtcagctaa
     4861 tggctgcatt ggccctgggg gcggaagcgg ttctcatggg gaccgtattc atggccgcca
     4921 aggagtctgt tgctcatccg aagttcaagc aggccttaat caatgccacg gaatgcgaca
     4981 cggtcttgat ccagcgttcc atcaagaatc agacccgagt ctttaggaac agaaccgcgg
     5041 atgaggtttt aaacctggag tctcgggggg catctttgga agaacttatg cctatgatca
     5101 gcggccagcg agggaagaac gcattgatgc tcggggatat tgatgagggg accatgacct
     5161 gtggtcaggg ggttggtttg ataaagaatg aactaaccgt caaagaggtc atcgatggta
     5221 tggttaaggg agcagaaacg cttctgaata ggctcaactc gctaaaccgt tcccaataac
     5281 tctaaataag gaggaagagg aagatgaaaa ccaagaaagg aatcccagtt attttttgtg
     5341 catgggtcat atttttcatt ttccaaagtc cggctgcagg ggaaaatgtc atcaatattg
     5401 gtttcaccgg tccgttgacc ggtccggcgg ccgccatcgg cattgactcc ctccatggaa
     5461 acatgatcgc cgccaaggaa atcaacgacg aaggaggcgt ggcagtggcc gggaagaagt
     5521 acaaagttac gatccagtcc tacgatgatg aaggagtcgc ggccaaggct gtagccggga
     5581 tgcaacgttt gaaggataag tacgaggttc cggttatcaa tcaaaacatc agcgggtccc
     5641 tcatggccat gctggaaaga aatgagaaga tgggaatcct gctgatcggt tttttcaaga
     5701 ttcccgatgc caccaagcgg ggcaacaaac tgatcctgcg ccatcaacag accattgatg
     5761 aagatgccca aggcctggcc aaggagaccg tcagggtttt aaaagccaag acctatgcca
     5821 tgatctccga tgtgggagat tacgggaaaa cgtcggttaa agcataccag caagccttcg
     5881 aaaagctcgg ggtaaagata gtggcgaatg agtggttgga tgggcgaacc cagactgatt
     5941 ttcgggggca actgaccaag atcaaggccg ccaacccgga cgtgatcatg gttaccgcct
     6001 atgatgaggc tacggccggg gtcatcaagc aggcccatga actggggatc aagaccccgt
     6061 ttgctgtttc cacgggcttt gcggctgcgg gcgagaaact taccgggcca gatttgatcg
     6121 aaggttattt aagacgagtc gagtttacga attcaaggcc tatgccgcct ggggtggccc
     6181 gttatcggac ccaattgtat ccggccatgg ggtataagga accgataggc ggctttggcg
     6241 tgaattgcta tgcgagtgtc catatcatcg tgcgggctat gcaaaaagct ggtacaacca
     6301 cagacgcctt taagatccgg caggccgttc cattggtggt ccccctcgaa gagaaatatg
     6361 actctacggg cttaaaagcc tttgccgaag atggagaggg gatcatgagc gcccagatcg
     6421 ggaggtacca gaaaggaatc gtggtgccgg tgaaatagga ttcccaaaca gagcgccttc
     6481 cagaaggccc ccctctcccc gctattctaa aggcgggttg gagggatttc agggaatcgc
     6541 tgctcaaaga aatttttctt cacaattttt aaagagcctg gaggtcacta tttacagggg
     6601 agcctttttc cgtggctgaa tttctgcaat ataccgtcaa tggtttgatt gccggcagca
     6661 cctatggcct tttggccctg gccatgaccc tgatctatgg catcctcgcg attcccgatt
     6721 tttccttggg ggcgatctac gccctggggg cgttcatttc attctatatc attcaatggc
     6781 tgggaccgga atactatctg ttcagcctgt ttccgatcat catcctggcc ggacttctcg
     6841 ggacttgcaa cgaaaggatc gtatttaagc cgcttgaaaa agccccccat gcagcgggtt
     6901 ttattgccgc cttgggtctt tattccattc tggagggggg ctggaatgtc tggttcgggt
     6961 cggactggag gaacatcaac tccccataca acgacatggc catcgcccta gggccgatct
     7021 ctctcaccgt acagcggctc atcttatttg ccgtctgtct cctctttgcc ctggggacct
     7081 ttctcctcat cttccggacc ctgacgggca aaaagatacg ggccttctcg gaaaacaggg
     7141 aagtggccca gctgatgggg gtcaatacgc gccgcgtatc cgccctgact tttttcatcg
     7201 gttacgccct ggccgggatt ggaggtgcgc tggtggcccc caccgccctg gtaggcgcaa
     7261 gcatgggatt gacgccgatc atcaagtcgt ttatcgtcgt cgccctggga gggttgggca
     7321 gtgttaccgg ggccatcgtg ggaggattta tattggggct gggagagaat ttcggcgctg
     7381 cctacatcag ctccatgtat aaggatcttt tctctttcgg ccttttttta ggggttttga
     7441 tcttgatgcc ccagggacta taccgtcggt aacctaacga tggcatataa tcaggccgaa
     7501 aatatgaatt caaccgatcg attgtttgct ttttgagaag tttcaataag ccaaaggaaa
     7561 aaggaatgcg aatcctgacc tatcttttcc tagggcttct ggctttgctg ttgcccgtgg
     7621 tgattcggtc gaattactgg ctgtacatgt tcaccctggt ggggatttac atcatcctcg
     7681 ttgccagcct ggacctgatt tatggttaca cggggctggt gtctttgggt catgcggcct
     7741 tcttcgggat tggggcctat gcctgcggga ttcttcagac caagttagga ttttcctttt
     7801 taccggcgtt gcttttggga gcctttttta cctgcattgc ttcggcggtt ttgggctggc
     7861 ccatgctccg gatcaagggc ccctattttg ccttgggaac gctggccctg ggcctaatgg
     7921 taaccatcgt catccataac tgggatagcc tgaccggggg ggttagtttg tcggggatcc
     7981 cccttcctcc ggtgattcaa ttgctgggct atacagtaga tttttcctcc aagcgaacct
     8041 attattattt tgttttgctt tttgcttttc tggccctttt tttcatccgt caaatcaccc
     8101 attcacggcc cggccgggcc ttccaatcca tccgggaaaa tgcggacctg gccgaagccc
     8161 tgggggtgga catcctgcgg tacaagctta tcgcctatgt catggccagc acgatcgcag
     8221 gtgttgccgg gggactgtat gctagctaca tgggttccat cgaaccggcc attgctggag
     8281 ggcacatgtc tttccatctc ctggtcatga tcgttatcgg cggagcgcgg accatcacgg
     8341 gagagatcgt gggaccctta ctgctctggt tcgtccccga atttctggag gcaacccagg
     8401 tttatcgtcc gctgtttttc ggtttcatcc tgctggtagt gatcatcttc atgcccatgg
     8461 ggattgcggg taagctgcgc tctcttcatc caaggatagc ccaatggatt ccctgaacct
     8521 ggaaagtcgg gctcctctct tgcgggtgga gaaattatcc gtttctttcg gccgcttgtt
     8581 ggctgttcat gatcttgcct tccagattca ttccggagag atatttggtc tcatcggccc
     8641 caatggagcg gggaagacca cggtgttcaa tgccatcacc ggtacagtcc cgctcagtga
     8701 ggggaagata cgtttttccg aaaccgatat caccgggttg aaaacccatc aaatcacgac
     8761 gaggggggtc gtgcgggcct accaggcggc cacgattttt ccccaaaccc cagtggggga
     8821 gcatgtgatg accggccttc actgccgcac tttgacctcg gtatgggggg ccttcctcca
     8881 cacccccttc agccgaaaag aagagggtca gaaccggaag aaagtaaaag agttgctgga
     8941 attttcgaat cttgcggatt tccgggaaca accggcatcc agcctgacct gggcccagca
     9001 aaagcgctta atgctggtta cggccctggc caccatcccg aaactgattt tgctggacga
     9061 gccggtggca gggatgaacg ccagcgaaat acaagaaatg atcgaattga tcgccacggt
     9121 ccgaaaggaa ggggtaaccg tctttatcat cgagcacaac atgaaggtca tgatgcagat
     9181 ttgcgatcgg atcctggtca tgaattatgg ccagaaactc agcgaaggaa tcccgaagga
     9241 ggttgcccgg gacccaaagg tcatcgaagc gtatctgggg tataggcaaa attgattcga
     9301 ttaagacaaa tcgatgtttt ttacggcaaa tctcaggctc tgcatggagt ctcgttgtct
     9361 gtcggggctg gtgaaatcgt ggccctgatt ggagccaacg gggcagggaa gaccacagtg
     9421 ctgaaggcga tttccgggtt gaccccctgc actgcgggag agatcatttt taaggaaaaa
     9481 tcgctcaagg ggctaaagcc ggaggaa
//
LOCUS       DASPRR010000440         4371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_107985, whole genome shotgun sequence.
ACCESSION   DASPRR010000440 DASPRR010000000
VERSION     DASPRR010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107985"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /gene="rho"
                     /locus_tag="VEL68_16125"
     CDS             complement(<1..1205)
                     /gene="rho"
                     /locus_tag="VEL68_16125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXZ37550.1"
                     /translation="MNIVELKEKKINELNKLARQLNVEGASGMRKQDLIFAILQAQTE
                     KNGLIYGEGVLETLPDGFGFLRSPDFNYLPGPDDIYVSPSQIRRFNLRTGDTIAGQIR
                     PPKEGERYFALLKVEAVNYEEPDLAREKILFDNLTPLYPNSRLSLEVPGEDNRSMRVM
                     DLLTPIGKGQRGLIVSPPRAGKTMILQSIANSVAQNHKDTVLIVLLIDERPEEVTDMQ
                     RSVHGEVISSTFDEPAQRHVQVAEMVLEKAKRLVEHQKDVIILLDSITRLARAYNTVI
                     PPSGKILSGGVDSNALHKPKRFFGAARNVEEGGSLTIVSTALIDTGSRMDEVIFEEFK
                     GTGNMEIVLDRKLADRRIFPAMDVNRSGTRKEELLIDSDDLNRIWILRKLLSDFNTVD
                     AMEFTLEKM"
     gene            complement(1566..2348)
                     /gene="pgeF"
                     /locus_tag="VEL68_16130"
     CDS             complement(1566..2348)
                     /gene="pgeF"
                     /locus_tag="VEL68_16130"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HXZ37551.1"
                     /translation="MGDQPRFLQIGAWKGERRIAHGFGTRQEGGRKPSRTDWRGKAVQ
                     VEKDIYPLLSLRQVHGDRVVLIEGNRQKIDEVWQLEGDALLTRTPGIALGVFTADCLP
                     IFFYDPIQGVIGIVHAGWRGTAQGVAGKAVEKMGETFGCKKANILAALGPCIGPCCYE
                     VDGPVKNAFISGGFPWNLVSVPRAEEKWLLDLYQANLYILENHGISRENIQVLKICTS
                     CHGDTFYSYRNADKTGGRQLNFIALRKKLAAVPAGYRQGESP"
     gene            complement(2505..3464)
                     /locus_tag="VEL68_16135"
     CDS             complement(2505..3464)
                     /locus_tag="VEL68_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXZ37552.1"
                     /translation="MNSILSFLVHSQDSSKRLDVFLSQKGPALSRSQIKKLIETGKVQ
                     IDGRKAKAGLRLRENEVVTLTIPEPRRLEIEAEAIPLKVLYEDRHLIVVDKPAGLVVH
                     PGAGNYSGTLVNALLHRCPDLAGIGGVLRPGIVHRLDKDTSGVLVVAKDDLAHRSLSD
                     QFRKHTAERNYVGIVFGQLSEEGQVDAPVGRHPTQRKKMSARPWKGREARTYWRVLER
                     FRSFNLAEFRLQTGRTHQIRVHLSSIGHPILGDPLYGGRKRLASVESLPLRQGLRRLK
                     RQALHATSLGFVHPVTGDVLKFSSPLPEDIQEAIALLRRMERG"
     gene            complement(3475..>4371)
                     /locus_tag="VEL68_16140"
     CDS             complement(3475..>4371)
                     /locus_tag="VEL68_16140"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXZ37553.1"
                     /translation="GGGARGLAHLGVLCALEQEKISFDLLAGTSMGALVGGMYALTPR
                     CDFVVERFRHYLESKEFQKTNPDFLREPDQETKPTFKGIFHRFATFIKKGVFYSQSLT
                     RKAPISEEDFNQNISFLLDPVDMEKTQVPLAVVALDLKTGEEVVLREGSLQKAVCASC
                     AIPGILPPVMLGERYLVDGGWINRVPIGPVRKMGADFAIAVDVAEGVNESLDFETGLD
                     IVLRTNEITQHSLSQLQMKEADIIIRPDLAGIRWSDFHRLEDCLRAGEGATKEKADEI
                     RGRIRRGKMKKAFSFRSGRSSS"
BASE COUNT          876 a         1261 c         1180 g         1054 t
ORIGIN      
        1 ctcattttct ccagcgtgaa ttccatcgcg tccacggtgt tgaagtcgga cagaagcttg
       61 cggaggatcc agatgcggtt cagatcgtcc gagtctatga gcagctcctc cttcctcgtg
      121 ccggagcggt tgacgtccat cgccgggaag attctccggt cggccagctt ccggtccagg
      181 acgatttcca tgtttcccgt acccttgaac tcttcgaaga tcacttcgtc catccggctg
      241 ccggtatcga tcagcgcggt ggagacgatg gtcaggcttc ctccctcctc cacgttccgg
      301 gccgcgccga agaaccgctt gggcttgtgg agggcgttgg aatcgacccc gcccgaaagg
      361 atctttccgc ttggcggaat gaccgtattg taagcccggg ctagccgggt gatgctgtcc
      421 aggagaatga tcacgtcttt ctggtgttcc accaaccgtt tagctttctc cagcaccatc
      481 tccgccacct ggacatggcg ttgggccggc tcgtcgaagg tggagctgat cacctctccg
      541 tgaacggaac gctgcatgtc cgtgacctct tccggccttt catcgatcag aagaacaatc
      601 agaaccgtat ctttgtggtt ttgggcaacg ctgttggcga tggactggag gatcatggtc
      661 ttgccggccc gtggggggct gacgatcagt ccgcgctgtc ccttcccgat gggagttaaa
      721 aggtccatga cccgcatgga cctgttgtcc tcccccggaa cttccaagct taggcgggaa
      781 ttggggtaga ggggagtcag gttgtcgaag aggatcttct ctctggctag gtcgggctcc
      841 tcatagttga ccgcctcgac cttcaggagg gcgaagtacc gctccccttc tttcgggggg
      901 cgtatctggc ctgctatggt gtcgccggtg cgcaggttga agcggcgaat ctgcgaaggc
      961 gagacgtaga tgtcgtcggg gccggggaga tagttgaagt ccggtgaccg gagaaagccg
     1021 aagccgtcgg gcagggtttc cagaacccct tctccgtaaa tcaggccgtt cttctcagtc
     1081 tgcgcctgca ggatggcaaa gatgaggtct tgcttacgca tcccgctggc cccttcaacg
     1141 ttgagctgcc tggccaattt gttcagctca ttgatcttct tctctttcag ctcaacaata
     1201 ttcatttgca ctcctcaaaa aaattatgat gttcttaaaa aattgccggc cggtatcacg
     1261 gtgaagtggc gagagagact cttccagatt gatccattta tggatttctt ttcgggtccg
     1321 tgtgctggga gggggaaaag tctacggtct caaaggcctg atcgattttt gcaccaccgc
     1381 tttacaataa aatctactga ttcctaagct gagcatgaga accttttacg gattctcaga
     1441 aaggacttcg ctggatcaga tttcaaggca gatttttttg gccaatccct gtggaaggat
     1501 ttttctggtt tattttagaa acagcctgaa cctttgtcaa gcacaatttt tgggttttta
     1561 gggggttatg ggctttcccc ttgccggtac ccagccggaa ccgcggctag cttcttcctc
     1621 agggcaataa agttcaactg tctccctccg gtcttatcgg catttcggta agaataaaaa
     1681 gtatccccgt ggcaggaagt gcaaattttt aagacctgta tattttccct tgaaattcca
     1741 tgattttcca gaatatagag gttagcctgg tagaggtcca ggagccattt ctcttccgcc
     1801 cggggcaccg aaactaggtt ccacgggaac cctccggaga tgaacgcatt cttcaccggc
     1861 ccatccacct catagcagca ggggccgatg caaggcccca acgcggccag gatattcgcc
     1921 tttttgcacc cgaaggtttc ccccattttt tcgacggcct ttccagcaac tccttgggcc
     1981 gttccccgcc agcctgcatg tacgatccca atcaccccct gaatgggatc atagaaaaaa
     2041 atcggcaggc aatcggcggt aaagaccccc agggcaatcc ccggagtacg ggtgaggaga
     2101 gcatctccct ccagctgcca gacctcatcg atcttctgcc tgtttccctc aatgaggacg
     2161 actcgatctc cgtggacctg gcggagagac aggaggggat aaatatcttt ttctacttga
     2221 accgcctttc cccgccagtc tgtgcgggag ggttttctgc caccttcctg ccttgtcccg
     2281 aagccatggg caattcgtcg ttcccctttc caggctccaa tctgcaaaaa cctcggctgg
     2341 tcgcccattg aatccttttc cctaatctga tttccgactt aatactcttg attggcggac
     2401 tttcttgaat cttccatggt tcggagcagg gccatttttc acttttccct ccgaatgatc
     2461 atctcttctc cattccgcat tctgcattcc ccgttccgca ttttctagcc cctttccatt
     2521 ctccggagga gggcgatggc ttcctgaata tcctcaggga gtggggaaga aaacttcagg
     2581 acatctccgg ttaccgggtg gacgaaaccg agagatgtgg catgaagggc ctgacgcttg
     2641 agcctccgga gcccctgccg gaggggcaat gattcaaccg aggccagcct cttccgtccg
     2701 ccatacaggg ggtcccccaa gatggggtgc ccgatggaag agagatgaac tcgaatctga
     2761 tgggttcgcc ccgtctgcag gcggaactcg gccaaattga aggagcggaa gcgctccagg
     2821 acccgccagt aggttctggc ttcgcggcct ttccagggcc gagcggacat cttcttgcgt
     2881 tgtgtcgggt gcctgcccac gggagcgtcc acctgacctt cttcggacaa ctggccgaag
     2941 acgattccca cataattcct ctctgcggta tgcttccgga attggtcgga tagggaccgg
     3001 tgggccaaat cgtccttggc tacgacgaga accccggagg tgtctttgtc caatcgatga
     3061 acgattcctg gccggaggac accgccgatt ccggcaaggt ccgggcagcg atggagaagg
     3121 gcgttaacca gggtgcccga gtagtttccg gcccccggat gcacgaccaa tcctgcaggc
     3181 ttgtccacca cgatcagatg gcggtcttcg tacagaacct tcaggggaat ggcctccgct
     3241 tcaatttcca gcctccgggg ctcgggaatg gtgagggtca ccacctcgtt ttcccgcagc
     3301 ctcaacccgg ccttggcttt tctcccatcg atttggacct tccccgtttc aatgagcttc
     3361 ttgatctgcg accgggaaag agccgggccc ttctgggaga gaaaaacatc cagccgcttg
     3421 gaggaatctt gggaatgaac caggaaagat aagatggaat tcatcctcgc cggatcaaga
     3481 agaagaacgg ccacttcgaa aggagaaagc ttttttcatt ttccctctcc ggatgcgacc
     3541 ccggatttca tccgcctttt ccttcgttgc gccctcgccg gctctcaaac aatcttcgag
     3601 acggtggaaa tccgaccagc gaatccccgc caggtccgga cggatgatga tatccgcttc
     3661 tttcatctgc aattgactca ggctgtgctg ggtaatttcg ttggtccgaa ggacaatgtc
     3721 cagcccggtc tcaaaatcca gggattcgtt gaccccttcg gccacatcca cggcaatggc
     3781 gaaatccgct cccatcttgc gcaccgggcc gatcggtacc cggttgatcc agccgccgtc
     3841 caccagatac ctttctccca gcatcaccgg gggcaggatt ccggggatgg cgcagctggc
     3901 gcagaccgcc ttttgcaggg acccttcccg caggaccact tcttctccgg tcttcaagtc
     3961 caaagccacc acggccaggg ggacctgggt cttttccatg tccaccgggt ccaataaaaa
     4021 actgatgttc tggttgaagt cctcttcgga gatcggggct tttcgggtca aggactggct
     4081 gtagaagaca ccttttttga tgaaggtggc gaaccggtgg aaaatcccct tgaaggtcgg
     4141 tttggtctct tgatcgggtt cgcggagaaa atccgggttg gtcttttgga actctttgct
     4201 ctctaggtaa tgacggaacc gttcgaccac aaagtcacag cgtggggtca gcgcatacat
     4261 tccgccgacc agggctccca tggaggtgcc cgccaggaga tcgaaggaaa tcttctcctg
     4321 ctccagggcg cacagaaccc ccaggtgggc tagccctcta gccccccccc c
//
LOCUS       DASPRR010000441        10334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1070031, whole genome shotgun sequence.
ACCESSION   DASPRR010000441 DASPRR010000000
VERSION     DASPRR010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070031"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VEL68_16145"
     CDS             <1..138
                     /locus_tag="VEL68_16145"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701585.1"
                     /note="catalyzes the formation of L-aspartate
                     4-semialdehyde from L-homoserine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXZ37554.1"
                     /translation="SVRGTEKAISYLTDTMDRVTVSGGKSNPMGAAAAILKDIIRVYQ
                     K"
     gene            263..1135
                     /gene="panB"
                     /locus_tag="VEL68_16150"
     CDS             263..1135
                     /gene="panB"
                     /locus_tag="VEL68_16150"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXZ37555.1"
                     /translation="MKKKKSIQDFYAMKEKGEQITYITSYDYPTATFAERAGIDMILV
                     GDSLGMCVYGYEGTMPVTMDQMIWHTEAVRRGAPNTFVIGDMPFLSYQTTDEAAVENA
                     GRFYKEARVDCVKLEGGVRVASRLKAIVDAGMLVMGHIGLTPQSSSQLGGFKAQGRTL
                     DSARAQVEDALAVQEAGAALILLEAIPPEVSGYIRDTLTIPVLSIGAGPHCDGQLLIV
                     SDMLGVFEAFTPKFVKRYAEVAKVCTEALTNYVEDVRAKRFPEPKHIYPMIPEELAKF
                     KEWTASQKKGKKSK"
     gene            1253..2620
                     /locus_tag="VEL68_16155"
     CDS             1253..2620
                     /locus_tag="VEL68_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HXZ37556.1"
                     /translation="MAMKSKAAKRSKKAVKKTKAPKALKSSKAPKITKTQRTAKASNA
                     PKIKGTPWKTDKWFVSPWNYAPEVVKQFKFPPAKDIKIHDITLRDGEQQAGIAYTRED
                     KLRITKALAEAGVHRIETGMPAVSKEDEAAIKEIVQANFGPEIFAFSRCMAADVKLAK
                     DCGVKGVVVEIPSSEHIIKHAYGWELQKAIDLSIEATLLCKELGLYTVWFPIDASRAD
                     LNWFLDLIEEIATKGHMDALAVVDTFGGISPHAVPYLIKKIRERVKKPLEPHFHDDFG
                     MGVANTLIALGCGCTVAHTTVTGIGERAGNAAMEELVLALLAMYGIDIGIKTEKFYSL
                     SKLVRELSRTTIPSNRPIVGETLLDVESGIVASWVRRCRAENPLELGPFLPSLVGQKP
                     VEIVMGKSSGIDCVAEWLEIINADASPEQREAIVQKVKQFSIEKKGLLTEGEFKQIVD
                     SVVHG"
     gene            2722..3024
                     /locus_tag="VEL68_16160"
     CDS             2722..3024
                     /locus_tag="VEL68_16160"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXZ37557.1"
                     /translation="MFEFIKRTIYFGAGLASMTAEKIEEAVAEIVKKGEITEKQGREL
                     VQDLKERSRNARHELGQRVEKMMEEALQKLKIPTRKEIDELKARIERLEKERGSQI"
     gene            3048..4109
                     /locus_tag="VEL68_16165"
     CDS             3048..4109
                     /locus_tag="VEL68_16165"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ37558.1"
                     /translation="MRTPDFERFRTVLLRNGEPDYVPLGDISVHPILKEAILGRPLRT
                     LEDEVGFWGRAGYDHVPLEQGLQLADVIRKQSMHTIEAHYALDREETSARSWATEGKG
                     LITTLADFEKFQWPDPDALDYSAFEKIGSLLPPKVKVVAILGKIFTNVWWLMGLEGMS
                     LSLADEPELVPRMFEKVGKFQFRVFENMLRYDWVGAVWHADDIAFSTALMVSPQILRR
                     HLFPWYKEMNRITHEKGRLAVFHSDGALQQVLDDIVACGFDGLNPIEPKAMNINEVKK
                     KYGSRLSLLGNIDLIYTLTRGTPEEVRAEVRQRIHDLAPGGGYAVASANSIPEYVPLA
                     NFNAMREATFEYGRYPIPV"
     gene            4219..5523
                     /locus_tag="VEL68_16170"
     CDS             4219..5523
                     /locus_tag="VEL68_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37559.1"
                     /translation="MDAMIGILMLAIYILAVVLIMKGESPIISLLVLAILWAVLAGVP
                     WKGEAGKDILSGVLERGGTAYASAIVIIVFGAWFGQTLVKTHIAENIIRGAIELAGDR
                     PTVVAMVVSITTGLLFTSIYGVGAAISIGVIALPIMMSMGIPPWVAAPAFTMAIGAGN
                     YVNLVEFNIFVPMFPGISYKQPYITFFFTGWAVYILAACAMSFYQLKIRGVRKLSAIN
                     VAPPQAARVPIPWYAYISPAIPVLLVILFKWSMIPAFIAGVFYALVSTHIGKRTLRES
                     VDLFHRAFYDAFPDIATIAALWIICGMLIIGGQLPQVQKVLSPVFTPFLPQAPLTLSI
                     FFAALAPLAIYRGPFAAVGTGAALLAMFLNANLINPVYLYCVWRGALCLQGSQDPTNS
                     WTLWTIGYTKVTHRQFLQTALPWGWLMVAINAFLAYLMVPWS"
     gene            5641..7803
                     /locus_tag="VEL68_16175"
     CDS             5641..7803
                     /locus_tag="VEL68_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXZ37560.1"
                     /translation="MMGGFKVRIGIDVGGTFTKAVVIHNDTLQIIGKSSVLTTHAAAE
                     GVAKGVIHVFQRALADFHVDPRDVVFLAHSTTQATNALLEGDLAPVGIIGMGSGGVGG
                     LLAKNQTFIGDIPLVTGRFLKTCHAFLNTRKLTREEARRSIEDLLSRGAKVIVASDSF
                     SVDDPRNELVVMETAQQLGVPATGGHDISKLYGLTIRTRTAVINAGILPKMLETANMT
                     EDSVRKAGIEAPLMIMRGDGGVMDVTEMRKRPILTMLSGPSATVAGALMYLRVSDGVF
                     FEVGGTSTNIGVIRNGRPTIKSAEVGGHRTYVNSLDIRVLGIAGGSMVRVKGKEIIDV
                     GPRSAHIAGLPYSAFAQPDEIEDPELVFIQPKEEDPSDFVAVRVKNGKVYALTNTCAA
                     NALGIVEPEHYSYGNPESARRAIAPLAAQMGVSVEETARRILDVATDKVIPVINALIS
                     EYALDRDQVVLVGGGGGAAALIPHSAKRMGFEFRIPQDAEVMSSIGVALAMVRDVVER
                     VILNPSVEDIAAIKQEARQLVMKACANPESIEVFVEINPQTGRVRATAIGTMEMRSQN
                     MCQILSPEECRRIASQSMNLPEEKVSLKASTGLVHVYQGTLEEKRWKFFNSRRFPVRV
                     LDNQGFVKVQRSDGSVFQFSGAGAIAGLKETWGNLTIYNGDSIIYPDLFLIVGSHILD
                     LTGVQSVDQALGVVSTELEGIPPDQPVAVVGIRGQRGL"
     gene            7803..8468
                     /locus_tag="VEL68_16180"
     CDS             7803..8468
                     /locus_tag="VEL68_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37561.1"
                     /translation="MNESLASFMAEVIVEATERRCACLMLRDDPFFRRLPSAKEGEAI
                     EWAFAAGQAAAQKITTEYGRSTERIASSLQVRVTRAETAMVAGKAVLFSEYRDRPPTI
                     TIYSRSVEETNRLIRENGLAKALGLEDVTPVHVAHELYHHLEAKKLTEGTCGYRVPTL
                     TLGPIRLRTGLPSLGEIAADRFARVVLNLKVPPRAIQFITIHHHNQEYAWHLLEELQG
                     LPA"
     gene            8943..9992
                     /gene="dctP"
                     /locus_tag="VEL68_16185"
     CDS             8943..9992
                     /gene="dctP"
                     /locus_tag="VEL68_16185"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ37562.1"
                     /translation="MKKTRRDFLKNTGAAAIGAAAVLAGTRKAHAQGAPIKWKAQTLW
                     NPQETPQKTFEDFCKRVKVLTNGRLEIEPFSAGAIVPTNETLTALQGNVLQAIHVWPG
                     YASGKNPAFAALCDLIFAYEHPWELDIFMEYKGGLELLRELYKPFGAYTVGYMFWGVE
                     SWPSKKPIRNMQDFKGLKIREPQGMEAEFMAKAGASVVVLPGTEIFSALDKGVVDATN
                     WATASINDKTGFHQVAPYFTYPGFHSMPVGDFTVRQAEWEKLPADIKQILTTACREWC
                     WDSVERIATEDIKLVAEAKAKGKPIPIAWTEEEKAKARQIAQQIWQGWKSKNEQTKRA
                     IEMQEAWLRELGRIK"
     gene            10078..>10334
                     /locus_tag="VEL68_16190"
     CDS             10078..>10334
                     /locus_tag="VEL68_16190"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXZ37563.1"
                     /translation="MRKILDLIDATNTRIGKLVSILSLGVAFIITYEILMRQVFVKPT
                     VWVAEGTVFGCGLVYMLGGAWTLLQDGHVRVDMLYHGLSRRR"
BASE COUNT         2333 a         2853 c         3129 g         2019 t
ORIGIN      
        1 agcgtgcggg gaacggaaaa ggccatctcc taccttaccg acaccatgga ccgggttacc
       61 gtctccgggg gaaagtccaa tccgatgggc gccgccgctg ccatcctgaa ggatatcatc
      121 cgggtctatc agaaatagag cgagggatgg gaaaaagaac tgagccgctt tgagcggaaa
      181 tcgttaaatc cctcccttgc agggaaatag aagcagggtt agaatagatc atcccatatt
      241 ccttttttcg ataggaggag ccgtgaagaa gaaaaaaagc atacaagatt tttatgccat
      301 gaaggagaaa ggcgagcaga tcacctatat caccagctat gattatccca ccgcgacctt
      361 cgccgaaagg gcggggatcg acatgatctt agtaggggat tccctgggca tgtgcgtata
      421 cggctacgaa ggaaccatgc ccgtgaccat ggaccagatg atctggcaca ccgaggccgt
      481 ccgccgcgga gccccaaaca ccttcgtgat cggcgacatg cccttccttt cttaccagac
      541 gaccgacgag gcggcggtgg agaatgccgg acgcttttac aaagaggcca gagtggactg
      601 cgtcaaattg gagggcggcg tgcgcgtggc cagccggctt aaggcgatcg tggatgcggg
      661 catgctggtt atggggcata tcgggctgac ccctcagagt tcctctcagc tgggcgggtt
      721 caaagcccag gggcgtaccc tggacagcgc cagagcccag gtggaagacg ccctggcggt
      781 tcaggaggcg ggagcggccc tgattcttct ggaagccatc cccccggagg tttccggcta
      841 tatccgggac acccttacaa ttccggtcct gagcattgga gctgggcccc actgcgacgg
      901 ccagctgctg atcgtcagcg acatgctcgg ggttttcgag gctttcaccc cgaaattcgt
      961 caagcgctat gcggaagtgg ccaaggtctg tacggaagct ctgacgaatt atgttgagga
     1021 tgtgagagcc aagaggttcc cggagcccaa gcacatctac cccatgattc ccgaggagct
     1081 ggccaagttc aaggaatgga cggccagtca aaaaaagggg aaaaaatcga aataagcatc
     1141 aagcaataag cacagggcaa ggctaaagac ctgcagcccg tacctgaaag ctgaccgctg
     1201 attgctgaac atttaggact gaccactaca cgacatgagg aggatcaaag ggatggcaat
     1261 gaaaagtaag gcggccaaga gatcaaagaa agcagtaaag aaaaccaaag cgccgaaggc
     1321 cttgaaatct tccaaggctc cgaaaatcac caagactcaa agaacagcga aggcctcgaa
     1381 tgcgccgaaa atcaaaggaa ctccctggaa gacggacaaa tggtttgtca gcccctggaa
     1441 ctacgcgccg gaagtggtga agcagttcaa gtttcccccg gccaaggata tcaaaatcca
     1501 cgatattacc ctgcgggacg gggagcagca ggcgggcatt gcttacacga gggaagataa
     1561 attgaggatc acgaaggctc tggccgaggc gggagtccac cggatcgaaa cgggaatgcc
     1621 cgcggtctcc aaggaggacg aggccgccat caaagaaatc gtccaggcca atttcggccc
     1681 ggagatcttc gctttctccc gctgcatggc cgcggacgtg aaactggcca aggactgcgg
     1741 ggtcaaaggg gtggtggtgg agattccctc cagcgagcac atcatcaagc atgcctacgg
     1801 gtgggagctg cagaaggcca tcgacctgtc catcgaagcc accctgctgt gcaaggaatt
     1861 ggggctttac acggtctggt tccccatcga tgcctcccgg gcagacctga actggttcct
     1921 ggacttgatc gaagagatcg ccaccaaggg gcatatggac gccctggccg tggtggacac
     1981 ctttgggggg atttcccccc acgccgtccc ctacctgatc aaaaagatcc gcgagcgggt
     2041 gaagaagcct ctggaacccc atttccacga tgacttcggc atgggcgtgg ccaacaccct
     2101 gatcgccctg gggtgcgggt gcacggtcgc tcataccacg gtgaccggca tcggggagcg
     2161 ggctgggaac gcggccatgg aggaactggt cctggctctc ctggccatgt acgggatcga
     2221 catcgggatc aagaccgaaa aattctattc cctctccaag ttggtgcgag aactctcgcg
     2281 caccacgatt ccctccaacc gtcccatcgt gggggagacc ctgctggacg tggaatccgg
     2341 gatcgtggcc agctgggtcc ggcggtgccg ggcggagaat cccttggagc tggggccctt
     2401 ccttccttcc ctagtgggac aaaaaccggt tgagattgtc atgggaaaaa gcagtggaat
     2461 cgattgcgta gccgaatggc tggaaatcat aaacgcagat gcctccccgg aacaacgcga
     2521 ggcgatcgta cagaaggtga aacaattctc catcgaaaag aaggggctgc tcaccgaggg
     2581 ggaattcaag caaatcgtgg acagcgtcgt ccacgggtaa aaagcatttg aagacagtgg
     2641 gattgaggtt tcagaaaagg catgatccgc gagaaggatt atgccttttc ttgtaaagaa
     2701 cgggttctac gaggaggttc tatgttcgaa ttcatcaaaa ggacgattta cttcggagcc
     2761 ggcctggcgt ctatgaccgc ggagaagatc gaagaagcgg tagcggagat cgtaaagaag
     2821 ggggaaatta ccgaaaagca agggcgggaa cttgtccagg acctcaagga aaggtcgcgc
     2881 aacgcccgtc atgaactggg gcaaagggta gaaaagatga tggaagaggc tttgcagaag
     2941 ctgaagattc ccacccggaa agagatagac gagctgaagg ccagaatcga aagactggaa
     3001 aaagaaagag ggagccaaat ctgactttcc agctgaggag ggcgcccatg cgaacgccag
     3061 attttgaacg tttccggacg gtattgctcc gcaatggaga accggattat gtccccctag
     3121 gagatatcag cgtgcatccg attttgaagg aggccattct gggtcggccg cttcgcacgc
     3181 tggaggatga agttggcttc tgggggagag cgggttacga ccatgttccc ctggaacagg
     3241 gacttcagct ggccgatgtg atccgcaagc agtccatgca taccatcgaa gctcattacg
     3301 ccttagaccg agaagagacc tctgctcgat cctgggcgac tgaaggaaaa ggcctgatca
     3361 ccaccctagc ggacttcgag aaattccaat ggccggaccc ggatgcccta gactacagcg
     3421 cctttgaaaa gatcgggtcc ctattgcctc ctaaggtcaa ggtggtggcc atcctgggga
     3481 agattttcac aaacgtctgg tggctcatgg gcttggaagg gatgagtcta tcgttggccg
     3541 atgagccaga gctggtcccc cgcatgtttg aaaaagtggg aaagttccag ttccgggttt
     3601 ttgaaaacat gcttcgctat gattgggtcg gcgcggtctg gcacgccgat gacatcgcct
     3661 tttccaccgc cctcatggtt agtccccaaa tcctgcgccg ccatctcttc ccctggtaca
     3721 aggagatgaa tcggattacc catgaaaagg gccgtctggc ggtgtttcac agcgacgggg
     3781 cgctgcaaca ggtgctggat gacatcgtcg cctgcgggtt cgacggactg aatcccatcg
     3841 agcccaaggc catgaacatc aatgaggtca aaaagaagta tggttcccgg ctctccctgc
     3901 tggggaatat cgacctgatc tataccctga cccggggaac tcccgaggag gtgcgggcgg
     3961 aggtgcgcca gcgaatccac gacctggctc caggcggcgg gtatgccgtg gcctcggcaa
     4021 actcgattcc cgagtacgtt cctctcgcga atttcaacgc catgcgcgaa gcaacctttg
     4081 aatacggccg ctatcccatc ccagtgtaag cctgaagaaa gaaggaacct tccctccagg
     4141 agaggcagcc ggccctggtc tttctcccgg aacctatcct cgagggagtg agtcaacaat
     4201 gaaaggggga gacgagaaat ggatgcaatg atcggaatcc tgatgctggc catttatatt
     4261 ctggcggtgg tgctcatcat gaagggggag agcccgatca tctccctcct cgtgctcgcc
     4321 attttatggg ccgttctggc tggagtgccg tggaaagggg aggcggggaa agacatcctc
     4381 tcgggggtcc tggaaagggg cggcacggcg tatgcttcgg cgatcgtcat catcgtcttc
     4441 ggtgcctggt tcggtcaaac cctggttaaa acccacatcg cggagaacat cattcggggg
     4501 gcaattgaac tggccggcga tcggccgacg gtggtagcca tggtggtatc catcaccact
     4561 ggacttcttt tcacctcgat ttacggggtc ggggcggcca tctccatcgg agtgattgcc
     4621 ctgccgatca tgatgtctat gggcattccg ccgtgggttg ccgcgcccgc tttcaccatg
     4681 gctatcggag ccgggaatta cgtcaacctg gtggagttca acatcttcgt tcccatgttc
     4741 ccgggaattt cctacaagca gccctatatc acctttttct tcaccggatg ggcggtgtac
     4801 atcttggccg cctgcgccat gtccttctat caactgaaga ttcggggggt gcgaaagctc
     4861 tcggcgataa acgttgcccc cccgcaagcg gcgagggtcc ccatcccctg gtatgcctac
     4921 atttctccgg ccattccggt tttattggtg atcctcttca agtggtcgat gatcccggcc
     4981 ttcatcgcgg gcgtatttta tgccctggta tcaacgcaca tcggaaaacg gaccctgcgc
     5041 gagagcgtgg accttttcca ccgggccttc tatgatgcct ttccggacat cgccaccatt
     5101 gccgccctgt ggatcatctg tggaatgttg atcatcggcg gccagctgcc ccaggtgcaa
     5161 aaggtgctga gcccagtctt cacccccttc ctgccgcagg ccccgttaac cctctccatc
     5221 tttttcgccg ccctggcccc gctggccatc taccggggcc cctttgcggc ggtgggaacg
     5281 ggggcggcac ttctggccat gttcctaaac gcaaatttga tcaatcccgt ttacctctac
     5341 tgcgtatggc ggggggcgct gtgccttcaa ggcagccagg accctaccaa ttcctggact
     5401 ctatggacca tcggctacac caaagtgacc catcgccagt tcctccaaac ggcgctgccg
     5461 tggggatggc tcatggtggc tattaacgct ttcctggctt atctcatggt tccctggtct
     5521 taagatgggc aaccggcctg acccgaaccc tgaggggaga gccggaaggt gacgctgact
     5581 tcccctccca tggtccccgg gggaaaggcg ccggcaaatt cagtcacagc aaggataaaa
     5641 atgatgggcg gtttcaaagt tcgcatcggg attgacgtgg gcggcacttt tactaaagca
     5701 gtggtcatcc ataacgacac cctccagatc atcggcaaat cctctgttct tacgacccat
     5761 gccgccgccg aaggggtggc caaaggggtg attcacgttt ttcagcgggc gctggccgat
     5821 ttccacgtcg atccccgtga cgtggtcttc ctggcccaca gcacgactca ggctaccaat
     5881 gccctgctcg agggggacct ggccccggtg ggcatcatcg ggatgggaag cgggggggtg
     5941 ggcggcctgc tggccaagaa ccaaaccttc atcggcgata taccgctggt gaccggccgg
     6001 tttctcaaaa cctgccatgc ctttctgaac acccggaaat tgacccggga ggaggctcga
     6061 cggtcgatcg aggacctgct gagccgaggc gcaaaagtca ttgtggcctc cgactccttc
     6121 agcgtcgacg acccccgtaa cgaactcgtg gtaatggaaa cggcgcagca actgggggta
     6181 ccggcaacgg gagggcatga catttcgaag ctctacggcc tcaccatccg gacgcggacc
     6241 gcggtgatca acgccggcat tctgcccaaa atgctggaaa cggccaacat gaccgaggac
     6301 agcgtgcgca aagcgggcat tgaggccccg ctcatgatca tgcggggaga cggtggggtc
     6361 atggatgtga ccgagatgcg caagcgcccc atcctgacca tgctatcggg gccctcggcc
     6421 accgtggccg gggcgctgat gtatctgcgg gtgtcggacg gggtattctt tgaagtcggg
     6481 ggcacgagta ccaacatcgg cgtgatccgc aatggtcggc cgacgattaa atcggctgag
     6541 gtcggagggc accgtaccta cgtgaattcc ctcgacataa gggttctcgg cattgccggc
     6601 gggagcatgg tgcgggtgaa aggcaaagag attatcgacg tggggccgcg cagcgcccac
     6661 atcgcgggat tgccatactc ggcgttcgcc cagcccgacg agatcgagga ccctgaactg
     6721 gtcttcatcc aacccaaaga ggaagacccc tccgactttg tggcggtccg ggtgaagaac
     6781 ggaaaagtgt acgccctcac caatacctgc gccgccaatg ccctgggaat cgtcgagcct
     6841 gaacactact cctacgggaa tcccgagtcg gccagacgcg cgattgcgcc tttggccgca
     6901 cagatggggg tttcggtcga ggagacggcg cgacgcatcc tcgacgtggc cacggacaaa
     6961 gtcattccgg tcatcaatgc cctgatttcc gaatatgcgc tggaccgtga ccaggtcgtc
     7021 ttggtcgggg gcgggggcgg ggccgcggcc ctcatcccgc acagtgccaa gcgcatgggc
     7081 ttcgagttcc gcatacccca ggatgcggag gtgatgtctt ccatcggggt ggccctggct
     7141 atggtccggg acgtggtgga gcgggtgatc ctgaacccct cggtggaaga catcgcggcg
     7201 atcaagcagg aggctcgcca gttggtcatg aaagcctgcg ccaacccgga atcgatcgaa
     7261 gtgttcgtgg aaatcaatcc ccagaccgga agggtccggg cgacggccat cggcacgatg
     7321 gagatgcgat cgcagaatat gtgccaaatc ctgagcccgg aggaatgccg acgcattgct
     7381 tcccagtcga tgaacctacc cgaggaaaaa gtctcgctca aggccagcac tggtctggtt
     7441 catgtttacc aggggacgct ggaggaaaag cgatggaagt tctttaatag caggcgcttt
     7501 ccggttcgcg tgcttgataa ccagggcttc gtgaaagtgc aacggtccga cggttccgta
     7561 ttccagtttt cgggagccgg cgccatcgcg gggctgaagg aaacctgggg gaacttgacc
     7621 atctacaacg gggacagcat catctacccc gatctatttc tcatcgtggg cagccacatt
     7681 ttggacttga ccggggtgca gagcgtcgat caagccttgg gggtggtttc cacggagctg
     7741 gagggaatcc ccccggacca gcctgtggcc gtggtaggga ttcgcggcca gcggggcctg
     7801 tgatgaatga gtccctggca tccttcatgg cggaagtcat cgtcgaggcg accgagcgga
     7861 gatgtgcttg cctgatgctc agggacgacc ccttttttcg ccgcctcccc tcagccaaag
     7921 agggggaagc gatcgaatgg gcatttgccg ccggtcaggc cgcagcccaa aaaatcacaa
     7981 ctgagtatgg gcggtcaaca gaaaggatcg cttcttcact ccaggtccgg gtaacccgtg
     8041 ccgagacggc catggtagcc gggaaagcgg tcctcttctc ggagtacagg gaccggcctc
     8101 caacgattac catctacagc cgctccgtgg aggaaaccaa ccgcctgatc cgggagaacg
     8161 gtttggcgaa ggccttggga ttggaggatg ttacccccgt gcatgtggcg cacgagctct
     8221 atcatcatct ggaggccaaa aagctgaccg aagggacctg cggatatcgg gtcccgactc
     8281 tgactcttgg cccgattcgc ttgcgcacgg gtcttccatc cctgggcgaa attgccgccg
     8341 accgttttgc tcgggtcgtg ctcaatctca aggtgccccc ccgggccatt cagttcatta
     8401 ccattcacca tcacaaccag gagtacgctt ggcatctcct ggaagagcta caaggacttc
     8461 ccgcgtaaca aggaagtttg gctcggggcg atgcttctaa ggccggaaat gcccaatttc
     8521 cgtgaagttt ggacttaccc cggggataca attcggctct aacttgacac ccaccggaga
     8581 atagggaaca ggccgcggta caggcccgac aatctcgata aaggaggata tggggaatgg
     8641 acatgtcaat ggggatatta atgctcgtcc tctatgcggt gggtgaagat cgcggggaaa
     8701 ggggagaatc cttgatggcc aggatcgcgg cttaaaaata gcaggaataa atttccaggt
     8761 ggagaggagg atcagatttt gtctttgttt ccgggacgcg atacccggag gggcgagaaa
     8821 gactcttgac tagcgagcta tatccgttaa aaatggaaag tgctttttgg gcggaggtga
     8881 attttcaaga cgggggggag attccgcagc ccccctgatt aaattttaga aaggagctgg
     8941 atatgaaaaa gacgcgaaga gattttttga agaacacggg ggcggcggcc atcggtgcag
     9001 cagccgtcct ggctgggacc cggaaggccc atgcccaggg tgcgccgatt aaatggaagg
     9061 cccagaccct gtggaatcca caggaaaccc cgcagaagac cttcgaggat ttctgcaagc
     9121 gggtgaaggt tctgaccaat ggccgcctgg agatcgaacc cttctccgcg ggcgccatcg
     9181 tgcccaccaa cgaaacccta accgccttgc agggaaacgt gcttcaggcc atccatgtct
     9241 ggcccggcta tgccagcggc aaaaatcccg cgtttgccgc cctctgcgac ttgattttcg
     9301 cctatgagca cccctgggaa ctggatatct tcatggagta caaggggggg ttggaattgc
     9361 tccgggaact ttacaagccc ttcggcgctt ataccgtggg ctacatgttc tggggcgtgg
     9421 aatcctggcc ttccaagaag cccatccgca acatgcagga cttcaaggga ttgaagatcc
     9481 gggaacccca gggcatggag gccgaattca tggccaaggc gggtgcctcc gtagtggtcc
     9541 tcccagggac ggaaatcttc tccgctctgg acaagggcgt ggtggacgcc accaactggg
     9601 ccactgcctc cattaatgac aaaaccggct tccaccaggt agccccttat ttcacgtacc
     9661 ccggcttcca ttccatgccc gtcggcgact tcaccgtccg gcaggcggag tgggagaaat
     9721 tacccgcgga catcaagcag atccttacca ccgcctgccg cgagtggtgc tgggacagcg
     9781 tagagcgcat cgccacggag gacatcaaac tggtggctga ggccaaggcg aaaggcaagc
     9841 cgatccccat agcctggacg gaggaggaaa aggccaaggc ccgccagatc gcccagcaga
     9901 tctggcaggg ctggaagagc aagaatgagc agaccaagcg ggccatcgaa atgcaggaag
     9961 cctggctgag agagttgggg cgcattaagt aaatcagcct acttgtgatt tggttcgtaa
    10021 tgaattcggg atcgggaggg aaggcattcc cctcccgccc gcgtatgggt ggagaagatg
    10081 agaaaaatcc tggacctgat tgacgccacc aacacccgga tcggcaagct ggtgagcatc
    10141 ctttccctgg gggtggcctt tatcatcacc tacgaaatcc ttatgcgcca agtcttcgtc
    10201 aaaccgaccg tctgggtggc cgaaggcacg gttttcgggt gcggattggt ctacatgctc
    10261 ggcggtgcct ggaccctatt gcaggacggc catgtgcggg ttgacatgct ctaccacggg
    10321 ctttcccgcc ggcg
//
LOCUS       DASPRR010000442        18550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1232399, whole genome shotgun sequence.
ACCESSION   DASPRR010000442 DASPRR010000000
VERSION     DASPRR010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18550)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18550
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232399"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VEL68_16195"
     CDS             <1..394
                     /locus_tag="VEL68_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXZ37564.1"
                     /translation="DSHQKWEKVICANAAASGVFVFRVNRVGKEAKQNFYGKSFCATP
                     EGDLVDQPSGAIEGVVLAAIDLSEIERTRRVWTFLRDRRPELYGEILADGRVPPQKAP
                     MAQDEKEEKNGKEFPSASSAVEKVLMNR"
     gene            391..2103
                     /gene="argS"
                     /locus_tag="VEL68_16200"
     CDS             391..2103
                     /gene="argS"
                     /locus_tag="VEL68_16200"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXZ37565.1"
                     /translation="MNEVTRAISEFVSREIGLPAEEVEKGIEVPRDPRWGDYSYPCFS
                     LAKVKRKAPALIAQEVARNFHPDSLLKECSSESAYLNFRIRRETWAPVVLKRIFSGGE
                     DYGRSLEGKGKTVLVEFSSPNIAKPFHVGHLRSTIIGHSLGEIYERLGYRVVRLNHLG
                     DWGKQFGEVITGFRHWGSPKELEAHPLKHLFEVYSRFHSEAESQTELHEEARAWFRKL
                     EDGDPDARQLWGRFREISIKEFKRLYRFLGVSFDSYDGESFYQDRLGELVDRLRASGL
                     LVESQGAWIIPLEAYSLPPALLLKKDEATLYLTRDIAAAEYRFRRWAFDKLIYVVGAP
                     QKLHFQQLFKVLELMGYDWNRRMVHVEFGHVLGMSTRRGEVVFLQDVIDEAVQRAERV
                     IEERGEDKEARIGKIPEGERKTIARAVGLGAIVFNDLRTRRAKDVEFDWDRILGFEGA
                     TGPYCQNAHVRCCGILRKFSGTVADRVDYAILSSDEEFDLIKRLGELPEVTRRAGEEN
                     EPSGIANYMLDLAMSLNAFLARYRVLGEESHVTQARVLLIHGVKTVLSICQRMLGMEV
                     LERM"
     gene            2103..2798
                     /locus_tag="VEL68_16205"
     CDS             2103..2798
                     /locus_tag="VEL68_16205"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXZ37566.1"
                     /translation="MESRRERLRKAAARRRPKEFSRGAEASPPPPVQKSNQRAFYVAA
                     GALLLAMVAFVCGVQMGKALSDLRGVDETRPLIHDRKGEAPPFRFMEKGKDPPSAREA
                     NAQPSEGEQSLKEPSPSQQSLRGVSDRPGVTPKAASLPPKEEGKPIPAKTKFTLQVGA
                     YNNSQEAQDLVQQLKKKGYEAYIAPGTAAAKGMLHRVRVGHFQTLQEARQFALAFEKK
                     ENLKPIISSLQNP"
     gene            3120..3803
                     /locus_tag="VEL68_16210"
     CDS             3120..3803
                     /locus_tag="VEL68_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ37567.1"
                     /translation="MIEMVHVSKAYEGNPKALFDITLKVEKGQFVYLFGPNGAGKSTL
                     LKLLYAAERPTAGEVRVNAFDVVRMKPRKVPSLRRTLGLVFQDLKLLQRRTAQENIAF
                     ALEALRAERGEILKKAIQALRLVGLERKAECLPSELSAGEQQRLAVARAVVNDPVLLL
                     ADEPTGSLSLTAAEETIRLFEKINLRGTTVVLATHNEGIVNLWPKEKIVLEQGRLSEG
                     PHPLPVAHD"
     gene            3859..4746
                     /gene="ftsX"
                     /locus_tag="VEL68_16215"
     CDS             3859..4746
                     /gene="ftsX"
                     /locus_tag="VEL68_16215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXZ37568.1"
                     /translation="MSGSFIRQACQNLTQNPWLNAITLGTIALSFLVLGLFLVIFLNT
                     KGLLEEWGSRIRVTAYLSSTVDLSQALRLGQKVRGLEEVQEVKYRSKEEALRILEERM
                     PERKEWLRGLPRNPLPASLEVRLKGEYQNAEGVQSLVKKLRGTPGIEDLQYGSDWLEK
                     FSAFMALLKVLGLSLGGLLLLATIIVISNTIRLNIFARREEIEIMRSVGATGLFIRAP
                     FYMEGVLQGLLGVVLALGMLYAFFLLFLARVYEPLKGLLGNFPIQFLSWEQMVAMGLG
                     GVVLGLFGTQVSVGRYLRL"
     gene            4743..5927
                     /locus_tag="VEL68_16220"
     CDS             4743..5927
                     /locus_tag="VEL68_16220"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXZ37569.1"
                     /translation="MTFRGGVPIFLLLILTGSSWAQGDSSSPPKGVDQSKKELGGIKK
                     RIQEEKQRVQDISKKESSVISQLNTLDRSLAEREKELKGLKKKLTAVDRKVKKANQDL
                     GSLTENIDVQEVFLLNRMVSLYKFGEMEAPQLLFSSRSYEDFLSTRRYLTCILEQDRE
                     LIGDYRQRQAVVGNHQQQLMEDEQELRSVKKKAEQKKGEIQRDLLQKGRLLDGVRQEK
                     QVHLAAIRELEAASAQLQGLINRLERELQEKAREKTFVPSGRKFTALRGKLPYPVKGK
                     ILSTFGRNENSKFNTFTVQKGIEIEAPLGAGIRAVHDGRVLYSDWFKGYGKILIIDHG
                     EGYYTLSGHASSLLKAVGEDVRSGDVVALVGDTGSLKGPCLYFEIRQRGQPLDPMEWL
                     AN"
     gene            6168..7505
                     /locus_tag="VEL68_16225"
     CDS             6168..7505
                     /locus_tag="VEL68_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXZ37570.1"
                     /translation="MSGWLSKKKILVVALVFFSLGIYMGLTHARRVGADIESAYGKLK
                     VLADVFAIVERNYVEPVKANNLINGAINGMLETLDPHSNYMAPEVYKEMQTETRGSFG
                     GIGFEITIRDKVLTVVAPIEDTPASRAGIQSGDMILRIDGKSTKDMTLVEAVKLMRGP
                     QGTQVTITLMRQGFTEPKDFTLTRAIIPIRSVRFKTLEPGYGYIKVSQFIERTFSDLE
                     NALNKLESKEKPLKGLILDLRNNPGGLLEQAVKVADLFLESGMIVYTEGRVEGQKMKF
                     FVQKKEKVQEYPMIVLVNGGSASASEIVAGALQDQGRAVILGTQTFGKGSVQTIIPLE
                     DGSALRLTTARYYTPNGRSIQAQGIAPDIAVADLVPEGKRSVAPRFMREKDLEHHLQG
                     DKEQAVPEKNKEPDKTPEASATPQSTEDPPLDRALELLKTWQILNKVSAKKGP"
     gene            7532..8215
                     /locus_tag="VEL68_16230"
     CDS             7532..8215
                     /locus_tag="VEL68_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HXZ37571.1"
                     /translation="MGDLEKALRYPLSGEGWGTKFALGGFLYILGNLLGFIPWVGWIF
                     TILLALVPLGYAYLVFQNHLRGLESPLPPWGGWAELFARGLSVFFISLGYWIIPAILY
                     WMGKVLWESGGVAAFLGVLFLMLGIGVGLVAFFLLPMALAFYGREEESFWAAFRWSGI
                     VEKIWMVQREYFIGWLASLLFFLSLLLIRTYFLYLGWILYALGIFYLTLAAAYFFGRV
                     CRVSMEGRR"
     gene            8485..9702
                     /locus_tag="VEL68_16235"
     CDS             8485..9702
                     /locus_tag="VEL68_16235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXZ37572.1"
                     /translation="MKIPLLDLKAQYQRIRDEVNEEIQKVLDSQVFILGPMVEALEKE
                     IAADCGVPHAIGVASGTDALLLSLMALNVRPGDGVITVPFTFFATGGVISRLGAVPVF
                     IDIDPATYNLDLNRLEDYLRKNQGAADFPKVVMPVHLFGQMTDMESLTEVSRRFGLRV
                     VEDAAQALGARQKIHSPNEEEKRGQEWTAGSAGDLGCFSFFPSKNLGGFGDGGMIITR
                     DEALARKLRMLRVHGSKTKYEHDLIGINSRLDALQAAVLRVKRKHLSRWTEGRRRNAE
                     RYRALFREAKIEPSLVSLPQEQKGFYHIYNQFVIRGGERDALREHLRQEEIGTEIYYP
                     IPLHLQECYRPLRYYPGDLPESERASREVLALPIYPELTLIQQRRVVRAIADFYSPQK
                     TRRGRRGPVLPKS"
     gene            9764..10408
                     /gene="lepB"
                     /locus_tag="VEL68_16240"
     CDS             9764..10408
                     /gene="lepB"
                     /locus_tag="VEL68_16240"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXZ37573.1"
                     /translation="MRKENKGSENPQIKKKSTFREYAEAILVALALALVIRTFVVQAF
                     KIPSGSMEPTLEIGDHILVNKFIYGVKIPFTSISFWAWENPKRGDVIVFIYPLEPDKD
                     FIKRVIAVEGDTVKIVNKKLYLNGVEAFDPHAVYKEDTLLPGDSQKRDNFGPITVPPK
                     KLFVLGDNRDRSLDSRFWGFVPLEDVKGRAFIIYWSWDGKDTTVRWKRLGQLIH"
     gene            10627..11100
                     /gene="greA"
                     /locus_tag="VEL68_16245"
     CDS             10627..11100
                     /gene="greA"
                     /locus_tag="VEL68_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXZ37574.1"
                     /translation="MEKLPMTKEGFAKLQEELDRLRRVERPQVLQAMSEAAAHGDISD
                     NADYRVAKERRDYIESRIKELDDKLARAQVIDTRDLATDKVVFGCTVLLGEMESDRQV
                     KYRIVGAHEADIRAGRISINSPVAKALIGHRLGDAIQVTVPSGMKEYRILAISTD"
     gene            11166..12542
                     /locus_tag="VEL68_16250"
     CDS             11166..12542
                     /locus_tag="VEL68_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ37575.1"
                     /translation="MGKILVSDDELSMRQFLEILLKKEGHQVVCAGDGQEAWVRFQAE
                     PFDLVISDIKMPKMDGLELLQKVKEQRPSLAVIMVTAYASPEDAIAAMKAGAYDYLTK
                     PFKLEEIKSVIHNALELTEGQAPAEAPVGIFCNIVGHSTKMVQIYNLVRQVGPTRTNV
                     LISGESGTGKELAARAIHQHSSRNGKPFVTINCSAIPENLMESELFGHVKGAFTGAVA
                     NKKGLFEIAHEGTVFLDEIGDLSPLIQVKLLRVIQEREFMRVGETQTINVDVRIISAT
                     NKNLEQEVIQGRFREDLFYRLNVVHLHLPPLRERREDIPLLAQYFLEKYSKELGKNVR
                     SISSYVLDTLMNYTFPGNVRELENIIERSVALEGSNIILPESLLLSEHRKEAIPKGLP
                     SVHLTPAGMDLEKELNQLEKEVLQQALQLSNGVIKKAAELLQLSFRSMRWKIQKHGLR
                     DFVQEIKE"
     gene            12641..14416
                     /locus_tag="VEL68_16255"
     CDS             12641..14416
                     /locus_tag="VEL68_16255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ37576.1"
                     /translation="MGFRLDSSDGKMMKIQKAEEEGRDGLFSRIQWLMLFRAVLVTLL
                     LGATVIFQLRESPFFRYVSPYPFYLLISFTYALTLLYALLLRRIQKLKIFAYGQILGD
                     LFFITLLIYITGGIASIFFWLYFFSIFSAGAILYRRGGLWIASASSILYGTLLDLEYY
                     GILLPPGSRELYSLDYQSTYVLYLITVNMIAFYLVAILSSYLAEQVRRKEEELKKRII
                     DYRQLERLYKHIVQNVVSGLITVDGEGRITSFNRMAEEITGYKFEEVYQEEVDSLFPG
                     LSAWSRSVGGNPGEGGNRLRFLRWETRFARKDGAHLILGFSISPLKDSVDREMGNILI
                     FQDLTRLREMEEHVKRSDRLAAIGKMAAGIAHEIRNPLASISGSIEILKEELGGSTQN
                     QALMGIVLREVRRLNSLIADFLLFARPISPGRERIHLNRLIEEIFQMISHSPDFNVLI
                     HLDTEFEDELYIQGDPNQVRQVFWNLLINAAQAMPKGGILRVRLRKGSSRGLLPGGRS
                     YGEVSVIDSGSGIGEEEIGKIFDPFFTTKEKGTGLGLSIVHSIVESYGGKIAVQSQKG
                     QGSVFSVFIPLADPSPAVSAQMNPN"
     gene            14682..17732
                     /locus_tag="VEL68_16260"
     CDS             14682..17732
                     /locus_tag="VEL68_16260"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXZ37577.1"
                     /translation="MHPRLLQLLIYSCFFLSGATGLIYEVVWAKYLSLIIGSTTAATT
                     IVLATFMGGLALGNHLLGRRADQVQNRLKFYGWLEMGIGIYCALSLQIINWGSELYVN
                     ISRASFPPGPFTHLLRLFLAGIALLVPTALMGGTLPVLARLWVRSIKTAACGVATLYS
                     VNSFGAVLGCLLAGFFLVRVLGISLSMLLAAGVNVLVGLVAIGVSSCPAQENSLQEPE
                     PNFSSTSPGSIRIMRLALVGATLSGAAAMIYEVGWVRLTALVWGSSTYSFTVMLAAFI
                     MGIALGSLLVRPLARRKLDLFWLFGLCELFVAVSMLLSIPLYAWLPYAFASLGNYLVR
                     TPESFPFYQLLVFLCSMAVMILPTVFQGMTLPLVSQVASDHLSRLGRSVGRVFSLNTL
                     GTLLGALLAGLLLMPRIGTRHTLEIGVALTLLAAALVFAGDFRTPRPRRIAGFAAGLA
                     ALAAYYAFAPAWDQNALNSGAFRRKNLQATFEEYRQFVGKGKILFQKDGADCTVAVIK
                     VPRGKDGSQLILVVNGKTDASSEGDLSTQLLTAHIPLVLHGSAQDVLLVGLGSGITAG
                     GALQHDIRRLDVVEISKEVLEAATLFSPYNDQALEDPRLHLVVEDGKTFLKTVPRRYD
                     VIINEPSNPWISGIGALLTEEFFWEARNHLNPGGILVQWIQGYEFSDELFKTTLATIR
                     KAFPHVSGWNSLTGDYVFVCSVEPFNPDLKAAAKRMGHPRVGKSLMRLKISQLSTLLS
                     LEMMTPQTLAEVSDQALTNTDNFPILEYDAPRAFFLGRGVQGIVHLDDRLRAHRWEDL
                     LLKKWIEAKGPLASSEYEDIIHYYDRWGSGNPYFPQAVLREWVAAYPQRKEAWEAMGR
                     MSTGHDPTARIQAWERAASLAPQDFHANRMYAESLAIRFMSGVSLFGRQEAAPVIRSL
                     DRCLSLKDAHPDLVLETKGLLLSKMGYWEEAGDLLQEAARQRLKRTQGGNSSDIASLH
                     AQAAQFYRLGGNKEKALSSAREALKLTPLHPQGRFELSQVEKMP"
     gene            18176..18541
                     /locus_tag="VEL68_16265"
     CDS             18176..18541
                     /locus_tag="VEL68_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXZ37578.1"
                     /translation="MLLKQKKGFTLIELMIVIAIIGILAAIAIPQFAAYRVRGFNATA
                     QSDLRNMITAQEAYYADKQIYVGAGDVAVTLSAYGYTRSSNVTATIVSSNSFTYQMSA
                     SHGSGDRTWTILGPGGAIN"
BASE COUNT         4258 a         4875 c         5466 g         3951 t
ORIGIN      
        1 ggattcccac cagaaatggg agaaggtcat ctgcgccaac gcggctgcca gcggagtttt
       61 cgtcttccgg gtcaaccggg tgggtaagga agccaagcag aatttttatg gaaaaagctt
      121 ctgtgccact ccggaagggg acctggtgga ccagcccagc ggagcgatcg agggagtggt
      181 cttggcggcc atcgaccttt cggagatcga gcggacccgg cgggtttgga cctttctgag
      241 ggaccggcgg ccggagctct acggcgagat ccttgccgat ggaagagttc caccgcagaa
      301 agcgccgatg gcacaggacg aaaaggaaga aaagaacgga aaagagttcc cctccgcgag
      361 ttctgcggtt gaaaaggttt tgatgaatcg atgaacgaag taaccagggc catttccgaa
      421 tttgtatccc gggaaatcgg gttgcccgct gaagaggtgg agaaaggaat cgaggtcccc
      481 cgggatccca gatggggaga ctattcctac ccctgtttct ccctggccaa ggtaaaacgg
      541 aaagcccctg ccctcatcgc ccaggaggtg gcccggaact ttcatccgga ttcgcttcta
      601 aaggaatgca gctccgagag cgcctacctc aacttccgca tccgaaggga aacctgggcc
      661 cccgtcgttc tgaaaagaat cttctccggg ggagaggatt acggcaggag cctggaaggg
      721 aaggggaaga ccgtactggt ggagttttct tctcccaaca ttgccaagcc ttttcatgtg
      781 ggtcatctgc gctcgaccat catcgggcac tccctcgggg aaatctatga gcgactgggc
      841 taccgggtgg tccgactgaa ccatctgggc gattggggaa agcagttcgg agaagttatc
      901 actggtttca ggcactgggg aagcccgaag gaactggaag cccatcccct gaagcatctg
      961 ttcgaggtct acagccggtt tcattctgaa gctgagtctc agacggaact ccatgaagag
     1021 gcccgggcct ggttcagaaa gctggaagat ggagacccag atgcccggca gctctggggg
     1081 cgcttccggg agatcagcat caaagagttc aaaagactct atcgatttct gggggtctcc
     1141 ttcgattctt acgacggaga gagcttttat caggatcgcc ttggtgagct tgtagaccgg
     1201 ttgcgggcca gcgggctcct ggtagagagc cagggggcgt ggatcattcc tctggaggcc
     1261 tactccctgc cccccgccct cctcctgaaa aaggatgagg ccaccctgta cctcacccgg
     1321 gatattgctg ccgcagaata ccgcttccgt cgttgggcgt ttgataaatt gatttacgtg
     1381 gtgggagccc ctcagaaact ccattttcag caactcttca aggtccttga actcatgggg
     1441 tatgactgga accggcggat ggtccacgtg gagttcggcc atgtcctggg tatgagcacc
     1501 cgccgggggg aagtagtttt cctgcaggat gtgatcgatg aagcggtgca aagagccgag
     1561 cgagtaatcg aggaacgggg ggaagacaaa gaggcgagga tcgggaaaat ccccgaagga
     1621 gaacggaaaa cgatcgcccg ggccgtgggc ctgggggcca ttgtcttcaa tgacctgcgc
     1681 acccggcggg ccaaggatgt ggagtttgat tgggaccgaa tcctggggtt cgaaggagcg
     1741 acgggtccct actgccagaa tgcccatgtc cgctgctgcg ggatcctgag gaagttttcg
     1801 ggaaccgtag ccgatcgggt ggactacgct attctttcca gcgacgaaga atttgacctg
     1861 atcaaacggc tgggagaact tcccgaggtg acccggcgag caggggagga gaatgagcct
     1921 tcgggaatcg ccaattatat gctggacctg gccatgtccc tgaatgcatt tctggcgagg
     1981 taccgggtac tgggggagga atcccacgtt acccaggccc gggtcctttt aatccacgga
     2041 gtgaagaccg tcctttcgat ctgtcagcgg atgctaggga tggaagtcct ggagcggatg
     2101 tgatggaatc gcgaagggaa agactcagaa aagccgccgc acggcgccga cccaaggaat
     2161 tctcccgggg ggcggaagcc tctcccccgc cacccgtgca gaagagcaat caaagggcct
     2221 tctatgtcgc cgctggggcc ctgcttctgg ccatggtggc atttgtctgc ggggtgcaga
     2281 tgggaaaggc cctcagcgat cttcgcgggg tcgacgagac caggccgctc atccacgacc
     2341 ggaaagggga agcccctcct ttccggttca tggagaaggg aaaagaccct ccatctgccc
     2401 gggaagccaa tgcccaaccg tcggaaggcg aacaaagctt gaaagaaccc agtccttccc
     2461 agcagagtct gagaggggtc agcgatagac caggcgttac gcctaaggcc gcgagcctcc
     2521 ctccgaagga ggagggaaag ccgattcccg ccaaaaccaa attcaccctt caagtggggg
     2581 cttacaacaa ctcacaggaa gcccaggatt tggtccaaca attgaaaaag aagggctacg
     2641 aggcttacat cgcgccggga accgccgcgg ccaaaggtat gttgcaccgg gttcgggtcg
     2701 gccactttca aaccctccag gaggcccggc aattcgccct ggcctttgaa aagaaggaaa
     2761 acctgaagcc gataatttcc agcttgcaga acccctaatc aattcttttt ccgtccgaat
     2821 atttttctgg gattgatcga ggcggcccta ttgaccaaga agcggagaag ggacttttca
     2881 gggggaaaaa attgggttga agaaaaggtg attcccttct tgtggtcaac cgaattgtat
     2941 gctaacataa caaaaataac caggatttta agaaaagaca attcggcacc gataaagtag
     3001 ccaagaagta tctccctccg gaaatacgag ctgatatcca gaatccatcc ggagacaaga
     3061 agggatactt tccagaatcc agggataagg agatagagcc agaaaagggt ttgaccgaaa
     3121 tgatcgagat ggtccacgta agcaaggctt acgagggaaa tccgaaagcg ctctttgaca
     3181 tcactttgaa ggtggaaaaa ggacagttcg tttatctctt tgggccgaac ggcgcgggaa
     3241 agagcaccct tcttaaactt ctgtatgcag ccgaacggcc aaccgcagga gaggtcaggg
     3301 taaacgcctt tgacgtggtc cgaatgaaac cgcggaaggt tccttcccta cggaggacgc
     3361 tgggcctggt attccaggac ctaaaactcc tccaaaggcg gacggcccag gagaatatcg
     3421 cttttgcttt ggaggcactg agggcggaaa gaggggagat tctgaaaaaa gccattcagg
     3481 ccttgcgttt ggtggggctg gagcgaaagg ccgagtgtct gccctccgag ctttcagcgg
     3541 gggaacagca gcggctggcc gtggcccggg ccgttgtcaa cgaccccgtc ctccttctgg
     3601 cagatgaacc caccgggagc ctaagcctga ccgccgccga agaaaccatc cgcctcttcg
     3661 agaagatcaa cctccgcggc accacggtgg tcctggccac acacaacgag ggaatcgtca
     3721 acttgtggcc caaagagaag atcgttctgg agcagggaag gttaagcgag ggcccgcatc
     3781 ctctgcctgt ggcccacgac tgaagaccgt cggcggggag cgaggccgct tcgagcgggg
     3841 cgggttaagt taacggaaat gagtggatcc ttcatacggc aagcctgtca gaacttgacc
     3901 cagaatccat ggctaaatgc catcaccctg gggaccattg ctctctcctt cctggttctc
     3961 ggactcttcc tagtgatctt cctcaatacc aaggggctgc tggaagaatg ggggagccgg
     4021 atccgggtta ccgcctacct gagcagtacc gtagacctga gtcaggccct gcggctgggg
     4081 caaaaggtcc gcggtcttga agaagttcag gaggtaaagt accggtccaa agaagaggcc
     4141 ctgaggattc tggaagaaag gatgcccgag cgaaaggaat ggctcagggg gctgccgaga
     4201 aaccccctgc ccgcttcttt ggaggttcga cttaaggggg agtaccagaa tgccgaggga
     4261 gtccagagcc tggtgaaaaa attgcggggg acccccggga tcgaagacct gcagtacggg
     4321 tccgattggc tggaaaagtt ttctgccttc atggctctat tgaaggtttt gggtttgagt
     4381 ttgggagggc ttctccttct ggcgacgatc atcgtgattt ccaacaccat ccgtctgaat
     4441 attttcgcca ggagggaaga gattgaaatc atgcgctcgg tgggcgccac gggtctcttt
     4501 atccgagccc ctttttatat ggagggagtt ctccagggac tcctgggggt cgtccttgcg
     4561 ctggggatgc tctacgcctt ttttctcttg tttctggcgc gcgtctacga acccctgaag
     4621 ggtcttttgg ggaacttccc cattcagttt cttagctggg aacagatggt cgccatgggc
     4681 ttgggaggag tggttctggg tcttttcggg actcaggttt cagtcgggag gtatctacgg
     4741 ctttgacatt ccggggaggg gtcccgatct tcctgctcct aattctaacg ggcagttcgt
     4801 gggcccaggg agattcttcc agtcccccca aaggagtgga ccagagcaaa aaggagctag
     4861 ggggaatcaa gaagaggatc caggaggaaa aacaacgggt ccaggatatc agcaagaaag
     4921 aatcctcggt catctcccag cttaacaccc tggaccgcag cctggcagag agagagaagg
     4981 aactgaaggg cctgaagaaa aagctcactg ccgtggaccg gaaagtaaag aaagccaacc
     5041 aagacctggg ttctctgacg gaaaatatcg acgtccagga ggtcttcctt ttgaatcgga
     5101 tggtgtctct ttacaaattt ggtgaaatgg aagcacccca gctgctcttt tcctcccggt
     5161 cctacgaaga cttcctgagc actcgcaggt atctgacctg catccttgag caggaccgag
     5221 aattgatcgg cgactaccgc cagaggcagg cggtcgtggg aaatcaccag cagcagctca
     5281 tggaagatga acaggaactg cggtcggtca agaagaaggc cgagcagaaa aagggcgaaa
     5341 tccagagaga cctgctccag aaaggccggc tcctcgatgg ggtgcggcag gaaaaacagg
     5401 ttcatctagc ggccatccgg gaattggaag ctgcctcagc ccagttacag ggcctcatca
     5461 accgtctgga gagggaactc caagagaaag cccgggagaa gacctttgtc ccttcgggaa
     5521 gaaagtttac cgcccttcgg ggaaagcttc cttacccggt gaagggaaaa attctcagca
     5581 ccttcgggag aaacgagaat tcgaagttca acaccttcac cgtccaaaaa ggaatagaga
     5641 ttgaagcccc tctaggggca gggattcggg cggtgcatga cggccgggtt ctatattcgg
     5701 attggttcaa agggtatggg aagattttga ttattgacca tggggaaggg tattataccc
     5761 tctccggaca cgcctcttcg ctgctaaaag cggtggggga agatgtgcgc tccggagacg
     5821 tggtggccct cgtaggggat acgggttcgc ttaagggccc ctgcctttat tttgagattc
     5881 gccagagggg ccaacccctg gaccccatgg agtggctggc caattaatcg gggagggaat
     5941 cagtccgaga ggcgtccgaa tgggacggaa gggatacgga gcgaaaggaa gcccttccat
     6001 ccccgtaaag attggcgagg gaaaaccttt tttgtttcga tgtttggcca tgttcaatag
     6061 agagattttc ccccctattg gagagaaaca aagcccgagg gtttctttga aattcgctcc
     6121 gggctgtaaa tctgaccgtt gaaaccagcg ggcagtaagg agagtcgatg agcggttggc
     6181 tgagtaagaa aaaaattctg gtagtcgcct tggtcttctt ttccctggga atctacatgg
     6241 gcctgaccca tgcccgtcgc gtcggggcgg atattgaatc ggcctacggg aagctgaagg
     6301 tcttagcgga tgtcttcgcc atcgtggagc gcaattatgt ggagcccgtg aaggcgaaca
     6361 acctcatcaa cggggcgatc aacgggatgc tggagaccct ggacccccac agcaactata
     6421 tggccccgga agtgtacaag gaaatgcaga cggagacccg ggggagcttc ggggggatcg
     6481 gatttgagat caccatccgg gacaaagttc tcacggtggt ggcgcccatt gaggacactc
     6541 cggcgtcccg ggcagggatc cagagcggag acatgattct gcgcattgac ggaaaatcca
     6601 ccaaagacat gaccctggtg gaagcggtga aactcatgcg cgggccccag gggacccagg
     6661 tgaccatcac cctcatgcgc cagggtttca ccgagcccaa ggatttcacc ctgacccggg
     6721 ccatcatccc catccgcagt gttcgcttca agaccctgga gccgggatat ggatatatca
     6781 aggtctccca gttcattgaa aggactttct ccgacctgga gaatgccctg aacaagctcg
     6841 aatccaaaga gaagcccttg aaggggttga tcctggactt gcgcaacaac ccgggaggtc
     6901 tgctggaaca ggcggtaaag gtggcggacc tgtttttgga atcggggatg atcgtctaca
     6961 ccgaaggacg ggtggaaggg cagaagatga agtttttcgt ccagaaaaaa gaaaaggtgc
     7021 aggaatatcc catgatcgta ctggtcaacg gggggagcgc cagcgcctcg gagatcgtgg
     7081 ccggagccct ccaggatcaa ggaagggcgg taatcctggg aacccagacc ttcggcaagg
     7141 gctcggtcca gaccatcatt cccctcgagg acggttcggc ccttcgcctg accacggccc
     7201 gttactacac gcccaacggc cggtccatcc aggctcaggg gattgccccg gatattgccg
     7261 tggcggacct agtcccggag ggaaagcgga gcgtcgcccc ccggttcatg cgggaaaagg
     7321 acctggagca tcatctccag ggggacaaag agcaggccgt tcctgaaaag aacaaagaac
     7381 cggacaagac gccagaggcc tcggccaccc cccaatctac cgaggaccct ccgctggatc
     7441 gggctttgga gcttctgaaa acttggcaaa ttttaaacaa ggtatcggcc aagaagggtc
     7501 catgatgatg gtttggacaa agggataaaa gatgggggat ttggaaaaag ctctccggta
     7561 tcccttgagc ggcgagggat ggggaaccaa atttgctctg ggcgggttcc tatacatcct
     7621 cggaaacctt ctgggattca tcccctgggt aggatggatt ttcacgatcc ttctggcctt
     7681 ggttccccta ggatatgcct atctcgtctt ccaaaatcat ctccgggggc tggaaagccc
     7741 ccttccgccc tggggaggat gggcggagct atttgcccga ggcctttccg tttttttcat
     7801 ctccctgggg tattggatca ttcccgcaat cctgtactgg atgggaaagg ttctctggga
     7861 gagcgggggg gttgctgcct ttctgggggt tctctttctc atgctaggga tcggagtcgg
     7921 cctggtggct ttcttcctcc tgcctatggc cctggctttc tacggccggg aggaggagtc
     7981 tttttgggcg gctttccgct ggagcggaat tgtggagaag atatggatgg tccagaggga
     8041 atattttatc ggctggctgg ccagcctgct ctttttcctg agcctcctct tgatcaggac
     8101 ttattttctc tatttaggat ggattttgta tgccctgggg attttctacc tcactctggc
     8161 tgcagcctat ttttttgggc gggtctgccg ggtaagtatg gagggtcgac gctgagcggc
     8221 cagggtcgtg gcccggaaga aagccaggat gactcctggc tttctttttc attggatcat
     8281 caggtacaca aagacgcagg gggaagactg agaagtgaga aggatagaat atttgggcag
     8341 gccagtttcc cgagccatga atccattctc ttgcaccccc tatccgcccc ggtaagcaaa
     8401 atggatggtt gagaaagacg ccttgctgct ctgccatctt tgccttttga attttaaaac
     8461 atcctttttt caaggaggcg acgtttgaaa attcccctgc tggatttgaa ggcgcagtat
     8521 cagaggatcc gggatgaggt caacgaggag attcaaaagg tccttgatag tcaagtcttt
     8581 atcctggggc ccatggtgga ggcgttggag aaggaaatcg ccgccgattg tggggttcct
     8641 catgcgatcg gcgtcgcctc ggggaccgat gccctgcttc tctccctgat ggccctgaat
     8701 gtgaggcccg gcgacggggt gatcaccgtc cctttcactt ttttcgccac cggaggggta
     8761 atctcccgtc ttggggccgt acccgtcttc atcgatattg acccggctac ctacaacctg
     8821 gacctcaacc ggctggaaga ttacctgagg aaaaaccaag gggcggcgga tttcccaaaa
     8881 gtggtcatgc ccgttcatct tttcggccag atgacggaca tggagagtct gaccgaggtg
     8941 tcacggcggt tcggactgcg ggtagtggaa gatgcggccc aagccctggg ggcccgccag
     9001 aaaattcatt ctccgaatga ggaagagaag cgggggcagg aatggaccgc aggctccgcg
     9061 ggggatttgg ggtgcttttc cttctttccc agcaagaacc tgggaggctt cggggacggc
     9121 ggcatgatca tcacccggga cgaggcgctg gcccgcaagc tgcgtatgct ccgggttcac
     9181 ggttcgaaaa ccaaatacga acatgatttg atcgggatca acagccgtct cgacgccctg
     9241 caggccgcgg tcctgcgggt caagcggaaa cacctgagtc gatggaccga agggcgaagg
     9301 aggaacgcgg agcgttatcg ggcgctcttc cgggaggcga agatcgagcc ttccctggtt
     9361 tcccttcccc aggaacagaa gggtttttac cacatctaca accagttcgt catccgggga
     9421 ggggagaggg acgccctccg ggagcacctc cgacaagaag aaatcgggac cgaaatttac
     9481 taccccatcc cgttgcattt gcaggaatgc taccgacctt taaggtatta tcccggcgac
     9541 ttgccggaat ccgaacgggc gtcgcgggaa gtgctggctt tgccgattta cccggagctc
     9601 accctgatcc agcagcggcg ggttgtcagg gccatagcag acttctattc accccaaaaa
     9661 acgcggaggg ggcggagagg gccggtcttg ccgaagagtt gaagacagat cggagggaag
     9721 gcgaggatga gaaaaatatg aaaagaaaaa atgcagggat gaaatgagaa aagaaaataa
     9781 agggtcagag aacccgcaga tcaagaaaaa atccactttt cgcgagtatg ccgaggcgat
     9841 cctggtggcc ctcgcgctgg ccctggtgat ccgcactttc gtggtccaag ccttcaaaat
     9901 cccctctggg tccatggagc ccaccctgga gatcggagac catattctgg tgaataaatt
     9961 catctacgga gtcaagattc ctttcacctc catttccttc tgggcttggg aaaaccccaa
    10021 gagaggggat gtgatcgttt tcatatatcc cctggagccg gataaagatt ttatcaaacg
    10081 ggtgatcgcg gtggaaggcg atacggtgaa aattgtgaac aaaaaactct acctcaacgg
    10141 ggtggaagct ttcgaccccc atgcagttta caaggaggat accctccttc ctggggacag
    10201 ccagaaacgg gacaacttcg gtccgataac cgtccccccg aagaaactct tcgtcctggg
    10261 cgacaaccgg gaccggagcc tggacagccg cttctggggg tttgtcccct tggaggacgt
    10321 gaagggacgg gcgttcatca tctactggtc gtgggacggg aaagatacga ccgttcgctg
    10381 gaaacgcctc ggccaactga tccattgagg cagaaagaaa aaggaggcaa aggggcgagg
    10441 aagggataag gggccaatgg gccctggatc gggcgctccc ccgaatcgcc cagaggtcct
    10501 ggagagcaat cggcccccgt agagaatatc tggctgcatg aatgttggac cgatgaaaaa
    10561 aagccggaaa gggagcaaat cggaaattgc cccttggggg atctaggaag aggaggaaag
    10621 acatcgttgg aaaagttgcc catgaccaaa gagggattcg ccaaactgca ggaggaactc
    10681 gaccgattga gacgggtgga acgaccccag gttttgcagg ccatgagcga ggcggcggcc
    10741 cacggagaca tcagtgataa tgccgattac cgggtagcca aggagcggcg ggactatata
    10801 gagagccgca tcaaggagct ggatgacaaa ttggcccggg cccaggtcat cgatacgcgg
    10861 gacctggcca cagacaaggt ggtcttcggt tgcacggttc ttctgggaga gatggaatcg
    10921 gaccggcagg tgaagtacag aattgtgggg gcccacgagg cggatattcg cgctggccgg
    10981 atctcgatca actccccggt ggccaaggcc ctcatcggac accgtctcgg ggatgccatt
    11041 caggtgaccg tgccttcagg gatgaaggag tatcggattt tggcgatcag caccgattga
    11101 gggaaggaaa tcggcaataa gggcgaatga ggtccttaaa ggtcctttcc caaggatggt
    11161 gagagatggg gaaaatccta gtatccgacg atgaactgag catgcgtcag ttcctcgaaa
    11221 tcctgctgaa gaaagagggg catcaggtgg tctgcgccgg ggacgggcag gaagcctggg
    11281 tccgatttca ggcggagccc ttcgacctgg tcatctccga catcaagatg cccaagatgg
    11341 acggcctgga actgctgcag aaggttaagg agcagcggcc ctccctggcg gtgatcatgg
    11401 tgacggccta cgcttccccg gaagatgcca tcgccgccat gaaggccggg gcctacgatt
    11461 acctgaccaa gcccttcaaa ctggaggaga tcaaatcggt catccacaac gccctagaat
    11521 tgaccgaggg gcaggctccg gcggaggctc ctgtggggat attctgcaac atcgtgggcc
    11581 acagcacgaa gatggtccag atctacaatc tggtccggca ggtgggcccc acccgaacca
    11641 acgtgctcat ctccggagaa agcgggaccg gaaaggagtt agcggcccgg gccatccacc
    11701 agcatagctc tcgaaacggg aagcccttcg tgacaatcaa ctgcagcgcc attccggaga
    11761 atctcatgga gagtgagctc ttcggccatg tgaagggcgc cttcaccgga gccgtggcca
    11821 acaagaaggg cctttttgag atcgcccacg aagggacggt cttcctggac gaaattggag
    11881 acctctcccc cctgattcag gtgaagctcc tgcgggtgat tcaagagcgg gaatttatgc
    11941 gggtgggcga gacgcagacc atcaacgtgg acgtacgcat catctcggcc accaacaaga
    12001 acctggaaca ggaggtcatc caagggagat tccgggaaga ccttttctat cggctgaatg
    12061 tggtccacct gcatctgcca cccctgcggg aaaggcggga agacatccct cttctggccc
    12121 agtattttct ggaaaagtat tccaaggaac tgggcaaaaa cgtgcggtcc atttcctcct
    12181 atgtcctgga taccctgatg aactacacct ttcccgggaa cgtgcgggag ttggaaaaca
    12241 tcatcgaaag aagcgtcgcc ctcgagggtt ccaacatcat cctaccggaa agcctgcttc
    12301 tctccgaaca caggaaagag gcgattccca aggggctacc ctccgtccac ctgacccctg
    12361 ccggcatgga cctggaaaag gagctcaacc agctcgagaa ggaggtcctc cagcaggccc
    12421 tccagcttag caacggggtg atcaagaagg cggcggaact cctgcaactc agtttccggt
    12481 ccatgcgctg gaagatccag aaacacggcc tccgggactt tgtccaggag atcaaagaat
    12541 agacctatgc taggttcgat atccttagaa ggaaagcccc cgaagccttc tttcctttga
    12601 atcgggcggc gaggaatttt ccccgccgga ggggggaggg ttgggtttca gattggattc
    12661 ctccgatgga aaaatgatga aaatccagaa ggccgaagaa gaagggcgag acggcctttt
    12721 ttcgagaatc caatggctca tgttattccg ggccgtgctg gtcaccctgc tcctcggagc
    12781 cacagtcatc ttccaacttc gagagagtcc cttctttcgg tatgtttctc cctatccctt
    12841 ctatctcctc atcagcttca cctatgccct gaccctcctc tatgccctgc tcctgcggcg
    12901 gattcagaag ttgaaaatct ttgcctacgg acaaatcctg ggcgacctct tctttattac
    12961 cctgctgatc tacattaccg gagggatcgc ctccatcttt ttctggcttt acttcttttc
    13021 catcttcagc gccggggcga tcctctaccg gagggggggc ctgtggatcg cttcggcgag
    13081 cagcattctt tatggaaccc tgctggacct ggaatactac gggatccttc tcccaccggg
    13141 aagccgggag ctttattccc tcgattacca aagtacttac gttctctacc tgatcaccgt
    13201 gaacatgatc gccttctact tggtggccat cctcagttcc tacctggcgg agcaggtccg
    13261 caggaaagag gaagagctca agaaacggat catcgactac cgccagctgg aacgcctcta
    13321 caaacacatt gtccaaaacg tggtgagcgg attgatcacg gtggatggag aagggcgaat
    13381 tacctccttc aaccggatgg ccgaggagat tacggggtac aaattcgaag aggtttacca
    13441 ggaagaggtt gattccctct tccccgggct ttccgcctgg agccggtccg tgggaggcaa
    13501 ccccggagaa ggtgggaacc gtctccgctt cctccgctgg gagacccggt tcgcaaggaa
    13561 agacggggct catctcatat tggggttctc catctccccg ctgaaggact ccgttgatcg
    13621 ggagatggga aacattctca tcttccagga ccttacccgg ctgcgggaaa tggaggagca
    13681 tgtgaaacgc tcggaccgtt tggcggccat agggaagatg gccgcgggca tagcccatga
    13741 aatccgcaat cctctggcct ccattagcgg gtccattgaa atcctgaaag aagaattggg
    13801 gggttcgacc cagaaccagg cgctcatggg gattgttctg cgggaagtcc gccgcctcaa
    13861 ttcgctcatc gccgatttcc ttctctttgc ccgtcccatc tctccaggaa gggagagaat
    13921 ccatctgaac cggctcatcg aagagatttt ccagatgatc agccacagcc cggacttcaa
    13981 cgttctcatc catctggaca ccgagttcga agacgaattg tacatccagg gggaccccaa
    14041 ccaagttcgc caagtcttct ggaatctgct cattaacgcg gcccaggcga tgcccaaggg
    14101 gggaatattg agggtgagat tgcggaaagg ctcttcccgg ggccttctac cgggaggcag
    14161 gagctacggc gaggtatcgg ttatcgattc cgggtcgggg atcggggagg aggagatcgg
    14221 caagatcttc gatcccttct ttaccaccaa ggaaaaaggc acgggtctgg gcctttccat
    14281 cgtccacagt attgtggaga gctatggggg aaaaatcgca gttcagagcc agaagggcca
    14341 aggctcggtc ttttccgttt tcatccccct ggcggacccc tcacccgctg tttcggctca
    14401 gatgaacccc aactgaggtg ttggaaaaat gtcccccctc tccgagagaa tcgccccgtc
    14461 agggagagta gccgccaacc ccttccagtc tctttaatca gatttaagtc atgattatca
    14521 tatccttttt catcaatggg agggtttagc ctgggtgagc tagagggacg aagcggccta
    14581 ttctcgggct cctcgctcgt ggcatgaatt ttgtaagaag acgggatgga aatgccgatc
    14641 cagcaggaag ctgaaactag attccattcc agggagaagg aatgcatccg agattactgc
    14701 agctcttaat ttacagttgt ttctttcttt ccggagctac cggactcatc tatgaagtgg
    14761 tctgggccaa atatttgtct ttaatcatcg gcagtaccac ggctgccacc actattgttt
    14821 tggccacatt catgggaggt ctggctctgg gaaatcacct tctggggcgg cgagcggacc
    14881 aagtccagaa ccggctcaag ttctatggtt ggctcgaaat gggcattggc atctattgtg
    14941 ccctttctct ccagatcatc aattggggga gcgagctata tgtaaacatc tctcgcgcct
    15001 ctttcccgcc aggtcctttc acccatctcc ttcgcctatt tctggccggg atcgcgcttc
    15061 tggttcccac ggccttaatg ggtggcaccc tcccagtgct ggcccggctg tgggtacgct
    15121 ccatcaagac cgcagcctgc ggggtggcca ccctttattc cgtaaacagc ttcggagcgg
    15181 ttttaggatg ccttcttgcg ggttttttct tggtccgcgt gttgggcatc tctctttcta
    15241 tgcttttggc ggcgggggtt aatgtcttgg tcggcttggt ggccatcggc gtcagcagct
    15301 gtccggccca ggagaactct ctccaggagc ctgaacccaa cttctcttcg acgagccctg
    15361 ggtcgatcag aatcatgcgc ctggccttag tgggagcgac tctctcggga gcagcggcca
    15421 tgatctacga agttggttgg gtccgactga ccgccctggt ctgggggtct tccacgtatt
    15481 ctttcaccgt catgctggcc gccttcatca tgggaattgc cctggggagc ctactggtta
    15541 ggcctctcgc tcgaaggaag cttgacctct tctggctctt tggtttatgc gaacttttcg
    15601 tggctgtctc catgctactg tccatccccc tctatgcctg gctgccgtac gcttttgcca
    15661 gcctgggtaa ttacctagtt aggaccccgg agagttttcc tttctaccag cttctggtct
    15721 ttctctgcag catggcggtc atgatcctcc caaccgtctt ccaagggatg accctcccct
    15781 tggtcagcca ggtggccagt gatcatcttt ctcggctggg ccgatcggtg gggcgagtct
    15841 tttctttgaa caccctgggt actcttctgg gggccctgct agcagggctc ctgctcatgc
    15901 cccgcatcgg cacccgccat accctggaaa taggagtggc cctaacgttg ttggcagccg
    15961 cgctggtttt tgcaggcgat ttccggaccc cacgacccag gcggatcgca gggttcgccg
    16021 ccggattggc ggccctggca gcttattatg ccttcgcccc ggcttgggat cagaacgccc
    16081 tcaacagcgg ggccttccga cggaagaatc tccaagctac cttcgaagaa taccggcagt
    16141 tcgtgggcaa aggaaaaatc ctgttccaga aggatggggc cgattgtacg gtagcggtca
    16201 tcaaggtccc ccggggaaag gatggatccc agcttatcct ggtagtaaat gggaaaacgg
    16261 acgccagtag cgagggggac ctttccactc aactgctgac cgctcatatc cctctcgtcc
    16321 ttcatgggtc ggcccaggat gttttgctgg tgggcttggg aagcggcatt accgctgggg
    16381 gtgcgctgca acatgatatt cgccgcttgg atgttgtgga gatctcgaaa gaggtgctgg
    16441 aggcggcgac cttgttctcc ccttataacg atcaggctct tgaggacccc cgtctccatc
    16501 tggtggtgga ggatggcaaa acattcttga aaacggttcc ccgtcgctat gatgtcatca
    16561 tcaacgaacc gagcaacccc tggatcagcg gaatcggggc ccttctcacc gaggaattct
    16621 tctgggaggc ccggaaccac ctgaatccgg gggggatcct ggttcagtgg atccagggtt
    16681 atgaattttc tgatgaactc ttcaagacaa ccttggccac gatccgtaag gcctttcccc
    16741 atgtttccgg gtggaacagc ctgaccgggg actatgtttt tgtctgtagt gtggaaccct
    16801 ttaacccaga cctgaaggcg gccgcaaaaa gaatgggcca tccgcgcgtg ggaaagagcc
    16861 tcatgcgcct gaaaatctcc cagctctcca ccctcctttc tctggagatg atgaccccgc
    16921 agactctggc cgaagtctct gaccaggcgt tgacgaacac cgacaacttt cccattctgg
    16981 aatatgacgc tccccgggct tttttcctgg ggcgaggagt gcaggggatc gtccacctgg
    17041 atgatcgctt acgcgcgcac cgctgggagg atttgctttt gaagaaatgg atcgaagcca
    17101 aaggtccttt ggcctcatcg gagtacgaag acatcatcca ttattatgat cgctggggaa
    17161 gcgggaatcc ctacttcccg caagcagttc tgcgggagtg ggtagcggct tacccccaga
    17221 ggaaagaagc ctgggaggcc atgggacgaa tgtccacagg ccatgatccc acggcacgaa
    17281 ttcaggcctg ggagcgggcc gcctctctgg ccccccagga ttttcatgcg aaccgcatgt
    17341 atgccgagag cctggctatc cgattcatga gcggagtttc cctgttcggc cggcaggagg
    17401 ccgcgccggt aatcaggtcc ctggaccggt gcctctcctt gaaggatgcc catccagacc
    17461 tagttctgga gacgaaaggt cttctcctat caaaaatggg atattgggag gaggccggag
    17521 acctcctgca agaagcagcc cggcagcggt tgaagcgaac ccaaggagga aattcttccg
    17581 acatcgccag cctccatgct caagcggcgc agttctatcg cctggggggg aataaagaaa
    17641 aagccctttc ctcagctcgg gaggcgctga agctgacccc cctccatccc caaggccgct
    17701 ttgagctctc ccaggtcgaa aagatgccct gaaacttttc gcttgcctag ggtccctcgc
    17761 agaagatggg gggtggctgt acttttttaa agatgggcct agagccactt ccttttggca
    17821 tggaaagact aggcggtaat gactgcccct cttcacttcc gcacgatttt ttacagatgg
    17881 cacaaaaatg acaaaaaaga acagaagagt ggcagttttt ggcacttgat tatattcatc
    17941 aggagtaggt tctttaagga atatccggat gtttgaaaaa aggaatttgt tatcaaggtg
    18001 ttataaaaat tctgggttta ttttacaggt ggcaatattt ttgcatacaa aagttagcaa
    18061 aagaatgaat ggccaaaagg aggtgaggtg ctaaaaaagt tcggcttatt attcaataag
    18121 gaaaaaagat ttggaccaaa caactcaaat taaaagatga aggagggaga agaaaatgtt
    18181 gttaaaacag aaaaaagggt ttactttgat cgagctgatg atcgttatcg ccatcatcgg
    18241 catcctggca gccatcgcca ttccccagtt tgccgcctat cgggtcagag ggttcaatgc
    18301 cacggcccag tcggacctgc ggaatatgat aaccgcccag gaagcgtact acgccgataa
    18361 gcagatttac gttggtgccg gtgatgttgc cgtaactttg agcgcctacg gctatacccg
    18421 ctccagcaat gttaccgcaa ccattgtgtc gtccaactct ttcacttatc agatgtcggc
    18481 gtcacatggg tcgggggacc gaacctggac gatcctaggc cctggcggag ccatcaatta
    18541 ggaggtcact
//
LOCUS       DASPRR010000443         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1554415, whole genome shotgun sequence.
ACCESSION   DASPRR010000443 DASPRR010000000
VERSION     DASPRR010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554415"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VEL68_16270"
     CDS             <1..214
                     /locus_tag="VEL68_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004015956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ37579.1"
                     /translation="EETAVEEARRCFNCGVCNLCDNCYIFCPDVAIQKGKEGELNIID
                     YDHCKGCGICMEECPRDVIVMEVEGR"
     gene            211..1365
                     /locus_tag="VEL68_16275"
     CDS             211..1365
                     /locus_tag="VEL68_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ37580.1"
                     /translation="MKKIMVGNHAVSWGVMLARAEVISAYPITPQTTIVEELSVLCAD
                     GRLKARFIPVESEHSAMACCIGSSAAGVRTFTATSSQGLALMHEMLHWATGARLPIVM
                     ANVNRSLGSPFNIWGEQSDSLAQRDTGWLQIYCESNQEVLDTVVQSFKIVEQVLLPVM
                     LVLDAFILSHTAEAVDIPDQKLADEFLPPFRPDLRLNAKDPHIFGCLAPPEYLMEFRY
                     KIQQAMLQSKEVCHRVDEEFGQAFGRKYGLVEPYRCEGADLILVTAGSVTSTARVAVD
                     ALREKGRKVGLAKLRLFRPFPKEELVEIVREADKIAVLDRNLSAGIGGIFAHEIRAAF
                     CNEKDLPPIYSYIAGLGGRDTTLQTLNEIIYQTYERRKPEEQSVWVGLRS"
     gene            1383..>2048
                     /locus_tag="VEL68_16280"
     CDS             1383..>2048
                     /locus_tag="VEL68_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ37581.1"
                     /translation="MREGYESTELMYSGHLACAGCGATISMRYALKALGEDTVIVLPA
                     CCWSIIGGPFPYAAVKVPLIHTAFETGASTASGVKAGLDMRGDTDTTVAAWVGDGGTF
                     DIGIQALSGAAERNDDIIYFCYDNEAYMNTGIQRSSATPWGTWTTTTPSNYPKGQPKK
                     NMMEIMAAHRIPYAATCSVAFPEDMISKVQKAKSIRGTKFIHIFSPCPTGWKAPSSQA
                     VKIA"
BASE COUNT          422 a          598 c          633 g          395 t
ORIGIN      
        1 cgaagagact gccgtcgagg aggcccggcg ctgcttcaac tgcggagtct gcaatctatg
       61 cgacaactgc tatatcttct gcccggacgt ggccatccaa aaagggaaag aaggggagct
      121 gaacatcatc gattatgacc attgcaaggg ctgcggaatc tgcatggaag agtgcccccg
      181 ggatgtgatc gtcatggagg ttgagggaag atgaagaaga tcatggtggg caatcacgcg
      241 gtctcctggg gagtcatgct ggcccgggcg gaggtgatct cggcctatcc gattaccccc
      301 cagaccacga tcgtggagga gctttcagtc ctgtgcgccg acggaaggct taaggccagg
      361 ttcatcccgg tggaatccga gcactcggcc atggcctgct gcatcgggtc ttcggcggcg
      421 ggggtccgga cattcactgc cacctcctcc caggggctgg cgttgatgca cgagatgctc
      481 cactgggcca cgggggcgcg gcttcccatc gtcatggcca acgtcaaccg gtccctgggg
      541 tctcccttca acatctgggg agagcagagc gacagcctgg cccagcggga cacggggtgg
      601 ctgcaaatct attgcgaaag caaccaggag gtgctggata cggtcgtcca gtccttcaag
      661 atcgtcgaac aggttctcct cccggtgatg ctggtcctgg acgcttttat tctttcccac
      721 accgccgagg cggtggacat tcccgatcag aaactggcgg atgagtttct tcctcccttc
      781 cgtccggacc tgcgtctgaa tgccaaggac ccccatattt ttgggtgtct cgctcccccc
      841 gagtatttga tggaatttcg ctacaagatt cagcaggcca tgctccagag caaggaagtc
      901 tgccaccggg tggatgagga gttcggccag gccttcggcc ggaagtacgg gctggtggaa
      961 ccgtaccggt gcgagggggc ggacctgatc ctggtcaccg ccggctctgt caccagcacg
     1021 gcccgcgtgg ccgtggatgc ccttagggag aaggggcgga aggtggggtt ggccaaattg
     1081 cgcctcttcc ggccttttcc gaaggaggaa ctcgtggaaa tcgtccggga ggcggacaag
     1141 atcgccgtgc tcgaccggaa cctctccgcg ggaatcggag ggattttcgc ccatgaaatc
     1201 cgcgccgcct tctgcaatga aaaagacctg cccccgattt acagctacat cgccggcttg
     1261 ggaggaaggg atacgaccct tcaaaccctg aatgaaatca tctaccagac ctacgaacgc
     1321 cgcaaacctg aggagcagag cgtctgggtg ggattgagaa gctagaagga agggagtttt
     1381 ccatgcgcga gggatatgag tctaccgagt tgatgtattc ggggcacctg gcctgcgccg
     1441 gctgcggggc cacgatttcc atgcgctatg ccttaaaagc cctgggggag gacacggtca
     1501 tcgtcctgcc ggcttgctgc tggtcgatca tcgggggtcc tttcccctat gcagcggtca
     1561 aggtcccgct gatccacaca gccttcgaaa ccggggcttc caccgcctcg ggggtcaagg
     1621 ccgggctgga tatgagaggg gacacagaca ccaccgtcgc cgcctgggtt ggggatggag
     1681 ggacctttga catcggcatc caggccctct ccggtgccgc ggaaaggaac gatgacatca
     1741 tctatttctg ttacgacaac gaagcctaca tgaacaccgg catccagagg agttcggcga
     1801 ccccctgggg aacctggaca accaccaccc cctccaatta cccgaagggg cagccgaaaa
     1861 agaacatgat ggaaattatg gccgcccacc gcattcccta tgcggcgacc tgttccgtgg
     1921 cttttcccga ggacatgatc agcaaggtcc agaaggccaa gtcgatccgg ggaacaaagt
     1981 tcatccacat tttttcgccc tgtcccaccg ggtggaaggc tccctcctcc caggcggtga
     2041 agatcgcc
//
LOCUS       DASPRR010000444        19000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_915282, whole genome shotgun sequence.
ACCESSION   DASPRR010000444 DASPRR010000000
VERSION     DASPRR010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 19000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19000)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19000
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915282"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VEL68_16285"
     CDS             <1..482
                     /locus_tag="VEL68_16285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HXZ37582.1"
                     /translation="RPNPQSAGEVLKVWGSVKESEGKYAKRNSLLDGIPLALPALERA
                     RRMSERASRVGFDWPDLGAVWEKVEEELRELKKAQKSPSRRAAEEELGDLLFTLVNWA
                     RFKEISAEEALRKANRRFARRFFEVEKGLRQKGKRPQDSTLEEMDRLWKQAKKAIDH"
     gene            complement(525..1100)
                     /gene="pth"
                     /locus_tag="VEL68_16290"
     CDS             complement(525..1100)
                     /gene="pth"
                     /locus_tag="VEL68_16290"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXZ37583.1"
                     /translation="MKLILGLGNPGRSYGWTRHNVGFILLDCLAKRHGIEVSRRGMKS
                     LYGRGRIGSEEVILAKPQTFMNLSGEAAQRLLQFFKLKPEMVIVLHDDLDLPWGQVRV
                     RVRGGDGGHKGIRSIIEALGNGGFVRFKIGVGRPEIPGQDPADFVLEPLTGGEKEEFK
                     KMIEGQVEALEVLILEGPQKAMDRFHKKIDQ"
     gene            complement(1121..1876)
                     /locus_tag="VEL68_16295"
     CDS             complement(1121..1876)
                     /locus_tag="VEL68_16295"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HXZ37584.1"
                     /translation="MEQRELEVALRIEKGKGAARRIRREGQIPAVLYGRRRQTFHLAV
                     KPEELKKILTSGARENTLIGLKVNGPGSDQVGNPVVMLKDLQVHPLSQSYLHADFYAV
                     AMDEKIEVDIPVRLVGKAEGVKLGGIQQQAMREIRVRCLPTQIPEFLDVDVSALKIGD
                     SLHVRDLAPPEKFDIVTDQNFTVASVVPPISEAKYDEIVSAPEGEREVAQPERIGEKK
                     EEPEVAEPAAKGGKEAKEVKEAKEPKEAKEPKK"
     gene            complement(1887..2816)
                     /locus_tag="VEL68_16300"
     CDS             complement(1887..2816)
                     /locus_tag="VEL68_16300"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXZ37585.1"
                     /translation="MRVFSGNSNVPLAKEISHILGTPLGEADVTTFSDGEILVDIHES
                     VRGMDVFVVQSTCTPGNTNLMELLIMIDAMKRASANRITAVLPYYGYARQDRKVSPRA
                     PITAKLVADLITAAGAHRVLTMDLHAGQIQGFFNIPVDHLFAAPVLLDYIRTRFPGDM
                     VIVSPDAGGVERARAFAKRLKASLAIIDKRRDSPNVAKFMNLIGEVKGLHAVIMDDIV
                     DTAGTIVEAASALKTKGAQGVYACCVHPVLSGPALQRLDDSIIQEVVVTNTIPLRPEA
                     ASKRKFKVLSVAKLLADAIQRIHCNDSVSSLFV"
     gene            complement(2865..2939)
                     /locus_tag="VEL68_16305"
     tRNA            complement(2865..2939)
                     /locus_tag="VEL68_16305"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2905..2907),aa:Gln,seq:ttg)
     gene            complement(3119..3958)
                     /gene="ispE"
                     /locus_tag="VEL68_16310"
     CDS             complement(3119..3958)
                     /gene="ispE"
                     /locus_tag="VEL68_16310"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXZ37586.1"
                     /translation="MKVLSPAKVNLHLEVLERRADGYHEVQTLMMRIDLFDEMEVRPG
                     GQGIRLIAEGEAIPGGPENLACRAAQLFCREAGIPGNFEVRLRKNIPVAAGLGGGSGN
                     AAAVLLALNELYPKKLDQKLLPVLGGRLGADVPFFLYQKPALARGKGDRLTAAKMPEG
                     LGFLLLVPPFRISTPWAYDAFDQMTGGKKKDGTHLENSYSTPRDLLTVLKNDLEIPAL
                     SRYPEIGLRKEELLRLGACGALMSGSGPVVFGLFASKGEAEETGKRFVLPEGWKSFAA
                     QGI"
     gene            complement(3960..5483)
                     /locus_tag="VEL68_16315"
     CDS             complement(3960..5483)
                     /locus_tag="VEL68_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXZ37587.1"
                     /translation="MGFEHERLGPASRRFGLVTLILVALVGLAAGLGLASLFPTRSLS
                     SPVELMGAKKVNVAATPSMISPNLFVDLAKRVKPGVVNVSTKKVVKGGGRVFRQFNPP
                     SRERDLFRDFFGEEFFNRFFGETPQRDFVQRSLGSGFIIDKEGYIITNNHVIEGASEI
                     RVRLSTEKEYDAEIIGRDAKTDLALIKIKSWNDLPVVELGDSEKVEIGEWVMAIGNPF
                     GLSHTVTVGIVSAKGRIIGTGPYDDFIQTDASINPGNSGGPLFNINGEVVGINTAIVA
                     TGQGIGFAIPINVAKEIIPQLKKKGKVTRGGIGVYVQRMTPELAKSFGLEKGEGALVA
                     DVIPGGAAEGAGIRRGDVITKFDGKEIHEMNELPRVVAATPVGKDVEVEMLREGKPLK
                     LKLKVGELKEEAAAPSEEKAQVELGMSVQEITPEMARQLQLREPGGVIVSQVESGSPA
                     EEAGVQRGDIIQEINGQSIRKLGDYQNALGKVKKDEMVRLLVKRGERNLYLAFRSNK"
     gene            complement(5496..5906)
                     /locus_tag="VEL68_16320"
     CDS             complement(5496..5906)
                     /locus_tag="VEL68_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HXZ37588.1"
                     /translation="MESGEEKKGFTIRDRRVSTQGEERKPEGPPKGEAASSAEESKSM
                     RGSPEPMPELPEVSFSSFILSLSTSALVHLGEVPDPVTQKMEKHLPLAKQTIDLLAML
                     MEKTKGNLSGDEEKMMEHLLADLRWRYVREAKKE"
     gene            complement(5972..6577)
                     /locus_tag="VEL68_16325"
     CDS             complement(5972..6577)
                     /locus_tag="VEL68_16325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXZ37589.1"
                     /translation="MLKALIGDGLDPFLQGTSKIAQRLGLRPNTLTFIGLGVNLLAAW
                     AIAVGEWLQGCSLIVLAGFFDILDGAVARNCRQASAFGSFLDSVLDRYSDLSLLVGLL
                     IYYSRRGMILYQILLGAALMGTALTPYTRARAETLIPKCNVGVMERPERILLVFLGAA
                     IESIMPIVVWILAIFTNVTVIQRVHYTWRQMEEKDQSKRKK"
     gene            complement(6593..7687)
                     /locus_tag="VEL68_16330"
     CDS             complement(6593..7687)
                     /locus_tag="VEL68_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXZ37590.1"
                     /translation="MEKIRLAVVGVGNCASSLIQGIEYYQTHEGAEKLGLMHFELGGY
                     QPGDLEVVAAFDVDKRKVGRPLGEAIYAKPNCTWEIMPQVTNSKTIVQMGDPLDGISP
                     HMRDYPENQTFLLADEKPCDVEKVLRDSGAEILLNYLPVGSESATRFYATCCLNTGVS
                     LINCMPVFIVSSPEWGRKFAERKIPVVGDDVKAQLGATILHRALTQLFVDRGVKMDRT
                     YQLNVGGNTDFLNMLNHTRLQSKKVSKTEAVQSILDSPLEPGNIHIGPSDYIPWQKDN
                     KVCFLRIEGRGFGGVPLNVELRLSVEDSPNSAGVTIDAIRCCQLARKRKIGGPLSSVS
                     AYTMKHPPEQIPDQEARQKLEEFIQGRRDR"
     gene            complement(7779..10037)
                     /locus_tag="VEL68_16335"
     CDS             complement(7779..10037)
                     /locus_tag="VEL68_16335"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXZ37591.1"
                     /translation="MIDLSNYKDYLSEKAHKVLSSAIHESQKRQHHYLGVEHIFLAIT
                     EVESSLFAEIAEKLNFSPREVVLSLREYLNSSKQVWGGGMKVPPSTKAIFKLAWDSAI
                     NSGRKVIETTDLLKAIFQEGQNIPVRILRGMGVEPNTATRLISVETKVIDEKSEDFKK
                     RYDLPPYLNNFGVNLNKLARLGKLPPIIGRDGEIRQMMEILCHRDRPNSVMILGEPGV
                     GKTALVEGLATRLELSPDSVPKRLRNFQIVNLQMNTLVAGTMFRGMFEDRIENVIKEV
                     KRRHNIILFVDEAHTLIGAGTAMGVPTDAATIFKSSLARGEVQIIGATTASEYKTHIA
                     EDEALARRFRIVTVLEPSVNEAREILRGIRPRLEKNYSVQIADEAIETALSMAPRYQH
                     NLRLPDKAINWLDTASVRVEMEDLPRPVGPQDVIQIISQETRIPQEMVFRDTTHRFKD
                     LEEALGHRVVGQREAISAVARNLRLNKGPLKERFERPDGVLLFLGPTGVGKTELGKSL
                     AEFLFGEERKMVRLDMSEYRDSAISVDKLIGMPRGIVGSERGGVLTNQIRDNPYTVVL
                     LDEVEKAHPDVLNLFLQVFDEGFLTDGRGRKVYFSDTVLIMTSNLGSDEFKRFTKPMG
                     FLVDSHHLDTVKKAVMKEAESHFSPEFLNRIDDIVVFSPLTQEEVEEIARRYLAAVHR
                     SLVPYGKSLQFSETALKILAESGFSLKYGARFLKRRIDEQVKIPITLHWQENSRFTID
                     AREGNMAVTWDS"
     gene            complement(10060..11175)
                     /locus_tag="VEL68_16340"
     CDS             complement(10060..11175)
                     /locus_tag="VEL68_16340"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HXZ37592.1"
                     /translation="MASRKDYYDILGIRRGATEEEIEKAYQKLARTYQTAPHPGNKTT
                     DFRFKEILEAYETLSDKNRRERYDRLGLDVPFQEGFWEVGLDEGDEENSLEGFEDFWE
                     QGSKGGNPGVFQGVERGRDIHFTLEIDLASAVRGVVKKVQILKENPCSVCQGEGFFAG
                     GPQKLCSLCGGAGQIQVGLPPSAFCIGCDRCRGTGKVSLQRCEACSGKGRIQQRIWVP
                     IRVPPGVDDHCRLYFSRMGNKARRGGPRGDLVAEVRVKKHPVLERKGEDLHLELPLAV
                     WEAALGAEVEVPTLGGKIKVKVPAGTQSGEAIRVPEGGIPLLGGSGRGDLVISPKIWV
                     PRRVDKKSRELLEELRRHLLVDAANRRGRPRKPGREK"
     gene            complement(11389..13281)
                     /gene="dnaK"
                     /locus_tag="VEL68_16345"
     CDS             complement(11389..13281)
                     /gene="dnaK"
                     /locus_tag="VEL68_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXZ37593.1"
                     /translation="MAKIIGIDLGTTNSVVAVMEGGDPKVIINEEGSRLTPSVVAFTK
                     DGNRLVGQVAKRQAVTNPENTVYSIKRFMGRRYNEVNEEMKMVPYKVIQDGSGAARVK
                     IKDKEYSPPEISALILQKLRKSAEDYLGEKVTDAVITVPAYFNDSQRQATKDAGVIAG
                     LNVRRIINEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGDNVVEVKSTNGDT
                     HLGGDNLDQRIIDFLAAEFKKDQGIDLSKDLMALQRLKEGAEKAKIELSSTMETEINL
                     PFITADASGPKHMNIKLTRGKFESLVMDLLEKSLPPCKQALEDAGIKTSQIDEVVLVG
                     GSTRIPKVQELVRQFFGKEPHKGVNPDEVVAAGAAVQAGVLGGEVKDVLLLDVTPLSL
                     GIETLGGVMTRLIDRNTTIPTRKSEIFSTAADSQTSVEIHVLQGERDMARDNRTLGKF
                     HLMGIPSAPRGIPQIEVTFDIDANGILNVSAKDNATGKQQAITITASSGLNKDEVEKM
                     VKDAEAHTADDKRRRQEVELRNQADTLVYQTEKTLSEHRDKIPVNEVGAIEAALKEAK
                     EAIQGGETNRIREKMELLTKASHRLAEIMYQQTASKQKPGAGPTGPQAGKPDEDVVDA
                     EFEEKK"
     gene            complement(13475..16105)
                     /gene="clpB"
                     /locus_tag="VEL68_16350"
     CDS             complement(13475..16105)
                     /gene="clpB"
                     /locus_tag="VEL68_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HXZ37594.1"
                     /translation="MRFDKLTLKAQEALQEAQAITEKHEHQQVEPEHLLLALILQTEG
                     IIPQVFQKLGVSIPALTTQVEEALDKIAKVYGGGAGQVYISPRLKKVMDSSFEEAERL
                     KDAYVSTEHMLLAIIEERDGSASRILLGLGASKDAVYKVLMSIRGSQRVTDQSPEEKY
                     QALTRYTRDLTDLARKGKLDPVIGRDDEIRRVLQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPETLKNKKLVALDLGALIAGTKYRGEFEDRLKALLKEITEAEGEII
                     LFIDELHTLVGAGAAEGAMDASNMLKPALARGELRCVGATTINEYRKHVEKDAALERR
                     FQPVMVSEPTVEDTIAILRGLKERYEVHHGVRIQDSAIVAAATLSHRYISDRFLPDKA
                     IDLIDEAASRLRIEIDSMPVEVDEVERKITQVEIERQALLKEKDKSSLERLEKIDQEL
                     AELRSQSEGMKAHWQKEKEAIQKIRTLKERMEEAKRQEQQFEREGNLAKVAEIRYGKA
                     SEFQKNLERENQALAALQKERKMLKEEVDAEDVAEVVAKWTGIPVSKMIEGEIEKLLK
                     MESRLKNRVVGQEEAIRLVSNSVRRARADLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFLFDDEQAMVRLDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVVLFD
                     EVEKAHPEVFNALLQILDDGRLTDGKGRTVDFKNTVIIMTSNIGSQWIQDQEARDYEE
                     MRRRVLEALRAHFKPEFLNRVDEVIIFHALTLENIKEIVEIQLRNLRKRLAERKISMT
                     LTERAKEFLAKEGFDPIYGARPLKRAIQRFIQDPLALKILEGEFSEGDTVEVDATQKN
                     GLVFSKPSAKPRSRAAGTRA"
     gene            complement(16232..16681)
                     /locus_tag="VEL68_16355"
     CDS             complement(16232..16681)
                     /locus_tag="VEL68_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXZ37595.1"
                     /translation="MAMIRWDPFREMSALQERMNRLMSDFRTRTPWTEEEMAQGGWVP
                     AVDIYETKEAIVLNVELPGVTKEDLTLEVKDNTLTLKGEKKLEKDVKEESYHRMERTY
                     GSFMRAFTLPSTVQQEKVKARFRDGILEVTIPKAEEARPKQIKVDVG"
     gene            complement(16870..17847)
                     /locus_tag="VEL68_16360"
     CDS             complement(16870..17847)
                     /locus_tag="VEL68_16360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HXZ37596.1"
                     /translation="MSEPDLEKDLFVPLDLPPSEAKDLVVAEKSAAIEVFDPLKRYLL
                     EIRQFPLLTREEERALAQKWREEKDRSAALRLVVSNLRLVVKIAFEYQRAYSNLLDLI
                     QEGNLGLMQAVKKFDPYREVKLSSYASWWIRAYILKFLIDNWSLVKIGTTQAQRKLFF
                     RLKKEKGRLEAMGFDPGPKLLAGALNVTKEEVTQMDQRLEGGDLSLNTPLEDNGRQTY
                     MDVLPGGDTPEETVTGTQFNEILGKKIEEFTLRLKPKEAFLLKNRLMADEPMTLQEAG
                     TQLKVSRERARQIEKKVADKLRAFLQGQFPDLEDIQVWFKKLGQEKKEL"
     gene            complement(17844..>19000)
                     /locus_tag="VEL68_16365"
     CDS             complement(17844..>19000)
                     /locus_tag="VEL68_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXZ37597.1"
                     /translation="RREVRGIGTGMIIDGNGHIITNNHVVNGATKVQVKIASGEEMEA
                     KIVGVDPKTDLAVIQIKLPPNTPFLKFGDSDKLRVGEWVVAIGNPRGLEQTVTAGIIS
                     AKHRAGILDPSSYQDFIQTDAAINPGNSGGPLLNLWGEVIGVNAAIVSESGGFEGIGF
                     AIPSNMATAVAESLIKTGKVTRGWLGMSLQEVTPSLARSLNLKSPKGALVAEVTKGGP
                     AEKAGIRRGDVIVNLDGGAIENANELRNRLAGTRVGKRVEVVLLRKGERITVQPVVEA
                     YLPSPRMAALELESRLGMEVKEISILEARKRRLNSRDGVLVARVDPQGPAAQAGLEAG
                     DIVYQINQQTLRGLKDYHRIVEQLRPGQEALLLVRDWRTGETGYLTVGVQ"
BASE COUNT         3712 a         5751 c         5009 g         4528 t
ORIGIN      
        1 cccgtcctaa cccccagagt gcgggagagg ttttgaaggt ctggggttcg gttaaagagt
       61 cggaagggaa atacgccaag cggaactccc ttttggatgg aattcctttg gcccttccgg
      121 ccctggaacg agcccggagg atgtcggaga gggcttcccg ggtcggtttt gattggccgg
      181 acctgggcgc ggtatgggaa aaagtggagg aagagctccg ggaattgaaa aaagcccaaa
      241 agtccccttc gcggcgggcg gcagaagaag agctggggga cctccttttc actctggtca
      301 attgggcccg ctttaaggaa atttccgccg aagaagccct gcgaaaagcc aatcgccgtt
      361 ttgcccgcag gttctttgag gtggagaaag gcctgcgcca aaaaggaaaa cgcccccagg
      421 attcgactct ggaagagatg gaccgcctgt ggaaacaagc aaagaaggcg atcgatcatt
      481 gatcattggc cattgcgcat tgatcattat tcaatgggca atggtcattg atcaattttt
      541 ttgtgaaatc gatccatggc cttctgagga ccttcgagga tgaggacttc gagggcttcc
      601 acttgtccct cgatcatctt cttaaactct tccttttccc ctccggtaag cggctccaga
      661 acaaaatccg cgggatcctg cccggggatt tcaggccggc cgactcctat tttgaaacga
      721 acgaatccgc cgttgcccag ggcttcgatg atcgagcgga ttcccttgtg tccgccatcc
      781 cccccccgga cccggacccg tacttggccc caaggcaggt ccaggtcatc gtggagaacg
      841 atcaccatct ccggcttaag cttgaaaaac tggagaagcc gctgagcggc ttctccagat
      901 aggttcataa aggtttgggg tttggccagg atgacctctt cggacccgat ccttccccgg
      961 ccgtatagag acttcattcc ccggcgggag acttcgatcc cgtgtctctt ggccagacag
     1021 tcgaggagaa tgaaacccac gttgtgcctt gtccagccat aactgcgtcc cggatttccc
     1081 aggcccagaa tgagcttcac tccaccccct cagcagcagg ctatttcttg ggctccttgg
     1141 cctccttggg ctccttggcc tctttgactt ctttggcctc tttgccccct ttggccgccg
     1201 gttcggccac ttcgggttct tccttcttct cgccaattcg ctcgggctga gccacctccc
     1261 gttccccttc cggagccgaa acgatctcat cgtacttggc ctcggagatg ggcgggacga
     1321 cgctggccac ggtgaagttc tgatcggtca cgatgtcgaa cttctccggc ggggccaggt
     1381 cccgcacatg cagggaatcg ccgattttca gggcgcttac gtccacatcc agaaactcgg
     1441 ggatctgcgt cggcaggcag cggacccgga tttcccgcat ggcctgctgc tggattccgc
     1501 ccagcttaac cccttcggcc tttcccacca gccggacggg gatgtccacc tcaatctttt
     1561 cgtccatggc cacagcataa aaatccgcat gcaggtagga ttgactgagg ggatgaacct
     1621 gcaggtcttt cagcatgacc acagggttgc caacctggtc gcttcccggc ccgttgactt
     1681 tcaacccgat gagggtgttc tcccgggccc cactggtcag gatcttcttc aattcttcgg
     1741 gtttgaccgc cagatgaaag gtttgcctcc tccggccata caggaccgcc gggatctgcc
     1801 cctcccgtcg aatgcgacgg gccgctccct ttcctttttc gatccgcagg gcgacctcca
     1861 gttctctctg ttccatgact cctcccttac acgaataggg aactcactga atcgttgcag
     1921 tgaatccgct gaatggcatc cgcaagcagt ttggctacgg ataaaacctt gaatttccgc
     1981 ttcgaggccg cctccggcct gagcggaatg gtgttggtca cgacaacttc ctgaatgatc
     2041 gagtcatcca accgctgcaa tgccggcccg gaaaggaccg gatggacgca gcaggcatac
     2101 accccttgcg cccctttggt cttcaaggcc gaagccgcct ctacgatggt gcctgcagtg
     2161 tccacgatat catccatgat cacggcatgc aggcccttga cttccccgat aaggttcatg
     2221 aacttggcca cattcgggct gtcgcgacgt ttgtcaatga tagccagact ggctttcagt
     2281 cttttggcaa aggcccgggc ccgctccact ccccctgcgt cgggagaaac aatcaccata
     2341 tccccgggaa atcgagtccg gatatagtcc agcagaaccg gagcggcaaa aagatgatcc
     2401 accggaatgt tgaagaaccc ctggatctgc ccggcgtgca aatccatggt gagcacccgg
     2461 tgggccccgg cggcggtgat taaatccgcc accagcttgg cggtgatggg agcccgcggg
     2521 gacactttcc gatcttgccg ggcatacccg tagtagggaa gaacggccgt gatccggttg
     2581 gccgaggccc gtttcatggc atcgatcatg atcaggagtt ccatcaagtt ggtgttcccc
     2641 ggggtgcagg tggactggac tacaaagacg tccatccccc ggacgctctc gtggatgtct
     2701 actaggatct ccccgtcgct gaaagtcgtg acgtccgcct cacccagggg ggtccccagg
     2761 atgtggctta tctccttggc caggggaaca tttgaattgc ccgaaaatac cctcatgtcg
     2821 tccaagtctt ttccctccct gaaacgggat tccccgagaa aacctggctg gggcgggagg
     2881 attcgaacct ccgaatgcgg gttccaaaga cccgtgtctt accgcttgac gacgccccag
     2941 tttcagaccg tgcctaaatt gcccaaagtg aactaaaatg gctaaagtca aaaaccggaa
     3001 aaccctatcg agtgcagatt tgcggagatc cgcgcaaata ataatgaaac ttcaattaaa
     3061 attgaaagga tatgaatctt tatcctgcaa atctgagttt atctgcgccc aatctttctt
     3121 atattccttg ggcggcaaag gatttccacc cttccgggag aacgaagcgc tttccggttt
     3181 cttcggcttc cccctttgag gcgaagaggc cgaaaacgac cgggccactc cccgacatga
     3241 gcgctccaca ggctcccaga cgcaacaatt cctctttcct cagcccaatc tcaggatacc
     3301 tggaaagggc cggaatttct agatcgtttt ttaggaccgt caaaagatcc ctcggcgtgg
     3361 agtaggaatt ctccagatga gtcccgtctt tcttttttcc cccggtcatt tgatcgaagg
     3421 cgtcataagc ccatggggtg gaaatccgga aagggggaac caatagcaaa aaacccaacc
     3481 cctccggcat cttggccgca gtcaaacgat cccctttccc ccgggccaaa gcaggtttct
     3541 gatagagaaa aaagggaaca tccgccccca gcctgccccc cagaaccggg agcaattttt
     3601 gatccagttt cttagggtag agctcgttca gggccagaag aactgcagcc gcgttgccgc
     3661 tccccccccc caaaccggcg gccacaggaa tatttttccg aagtcggacc tcgaaattcc
     3721 ccggaatgcc ggcctcccgg cagaaaagct gagccgcccg gcaggcgagg ttctccggcc
     3781 caccgggaat cgcttctcct tccgcaatca ggcggatccc ttgacctccc ggacggacct
     3841 ccatctcgtc aaagaggtca atcctcatca tgagggtctg gacctcgtgg tagccgtccg
     3901 cccgcctctc cagtacctcc aggtggaggt tgacttttgc cggggagagg accttcattt
     3961 tatttattgg aacggaacgc caggtaaagg ttccgttccc cccgcttcac cagcaggcgg
     4021 accatttcgt cctttttcac tttcccaagg gcattctgat agtcgccgag tttgcggatg
     4081 gactggccgt tgatctcctg gatgatgtcc cctctctgga cccccgcttc ctccgccggg
     4141 cttccggatt ccacctggct cacgatcacc cctccgggct ctcggagctg tagctgccgg
     4201 gccatctccg gggtaatttc ctggacgctc atgccgagtt caacctgggc cttctcctca
     4261 ctgggagcgg cagcctcctc tttcagctcg cccactttga gcttcagctt cagcggcttt
     4321 ccctcccgca gcatctcgac ttccacatct ttccccactg gggtggcggc cactacccgg
     4381 ggtaattcgt tcatctcgtg gatctctttt ccgtcgaact tggtaatgac atcgccccgg
     4441 cgaattcctg ccccctcagc cgcgcccccc ggaataacat cggctaccag cgccccctcg
     4501 cccttttcca ggccgaagga cttggccagt tcaggggtca tccgctggac atacacccca
     4561 attccgccgc gggttacctt ccccttcttc ttcagctggg ggatgatctc cttggccaca
     4621 ttgatgggaa tggcaaagcc gattccctgg cccgtggcta cgatggcggt attgattcct
     4681 acgacttccc cgttgatgtt gaagagcggt cctccgctgt tcccggggtt gatggaggcg
     4741 tcggtctgga tgaaatcatc gtaaggaccg gtgccgatga tccggccttt cgcgctgacg
     4801 atgcccacgg ttaccgtgtg ggaaaggccg aaggggttcc caatggccat gacccactcg
     4861 ccgatctcca ccttttccga atcgcccaat tctaccaccg gaaggtcatt ccaggacttg
     4921 atctttatca gagccaagtc ggttttggca tcccgcccga taatttcggc gtcatactct
     4981 ttttccgtgg aaaggcggac ccggatctcg ctggctcctt cgatgacgtg attgttggtg
     5041 atgatatacc cttccttgtc gatgataaac cccgagccca gggagcgctg gacgaaatcc
     5101 ctctggggag tctccccgaa aaagcggttg aaaaactctt ctccgaagaa atcccggaat
     5161 aggtcccttt cccgggaagg cgggttgaac tggcggaaga ctctcccccc gcctttgacc
     5221 accttcttgg tactgacgtt caccaccccc ggtttgaccc gcttggccag gtccacgaag
     5281 aggttgggag agatcatgga aggagtggcc gccacattga ccttcttggc tcccatcaat
     5341 tccaccgggc tggataagga acgggtcggg aaaagggagg ccaatccgag cccggctgcc
     5401 agtcccacca gggctaccag gatcaaagtc accagtccga accgccggga ggctggcccc
     5461 aatcgttcat gttcgaaacc catgattcat ctcctctatt cttttttcgc ttcccgcaca
     5521 tacctccagc gaagatcggc cagaaggtgc tccatcatct tttcttcgtc gcccgaaaga
     5581 ttccctttgg tcttttccat gagcatggcc agcaggtcga tggtctgctt ggccaggggg
     5641 agatgttttt ccatcttctg ggtcaccgga tcagggacct ctcccaggtg gacaagcgcc
     5701 gaggtactca gcgacaggat gaaagaagag aaggagacct ccgggagctc aggcatcggt
     5761 tcagggcttc cccgcatgct cttcgactcc tccgctgaag aagcggcctc gcccttcggg
     5821 ggcccttccg gttttctttc ttccccttga gtggaaaccc ttcggtcccg gatggtgaat
     5881 cctttttttt cctccccgct ttccatggtg cctcctaata cagcctttac gcggtgcgtt
     5941 cggggaacgc accctacatc cttatggtac cctacttttt gcgtttggat tggtccttct
     6001 cctccatctg gcgccaggtg taatggaccc gctggatcac cgtgacgttg gtgaaaatgg
     6061 ccagaatcca cacgacgatg ggcatgatcg attcgatcgc cgcccccaaa aaaaccagca
     6121 agatccgctc aggcctttcc atgaccccca cgttgcactt tgggatcagg gtctcggccc
     6181 gggcccgggt gtaaggagtc agggccgtcc ccatgagcgc cgccccgaga aggatttggt
     6241 acaagatcat ccctcgacgc gaataataga tgagcaaacc gacaaggagg ctcaaatcgg
     6301 aataccggtc caggaccgag tccaggaaag aaccaaaggc actggcttgg cggcaattcc
     6361 gagcgaccgc cccatccaga atatcaaaga agccggccag gacgatgaga ctgcacccct
     6421 ggagccattc gcccacggca atggcccacg ccgcgagaag gttcacgccc agaccgataa
     6481 aagttagggt gttgggacgg agacccaagc gctgagcgat tttggaagtt ccctgcagaa
     6541 acggatcgag gccgtccccg atcagtgctt tgagcatagc cggatgcgct tcctacctgt
     6601 cacgacggcc ctggatgaat tcctccagct tctgcctagc ttcctggtct ggaatctgtt
     6661 cgggcgggtg cttcatggta taagctgaaa cagatgacag gggtcctccg atcttccgtt
     6721 tcctggccag ctggcagcag cgaatggcat cgatggtcac cccggcgctg ttgggagaat
     6781 cctccacgga gaggcggagt tccacgttca ggggaacccc cccgaatccc cggccttcga
     6841 tccgcaggaa gcagaccttg ttatccttct gccaggggat ataatcgctc gggccgatat
     6901 ggatattgcc gggttctaag ggagaatcca ggatggactg gaccgcctcg gttttggaaa
     6961 ccttcttgga ctgcagtcgg gtgtgattca gcatattgag aaaatccgtg tttcccccca
     7021 cgttcaactg gtacgtccgg tccatcttga ccccccgatc cacgaaaagc tgggtcaggg
     7081 cccggtggag aatggtggcg ccaagttggg cttttacatc atcgcctaca acgggaatct
     7141 tccgctcggc gaacttgcgg ccccattcgg gggaggagac aatgaagacc ggcatgcagt
     7201 tgatgaggct caccccggtg ttcaaacaac aggtcgcata gaatcgggtg gcactctccg
     7261 accccacggg aaggtagttc agaagaatct ccgcgccgga gtcccgcagg actttttcca
     7321 cgtcacacgg tttttcgtcg gccaggagga aggtttggtt ttccggataa tcccgcatgt
     7381 ggggagaaat cccgtccagg gggtctccca tttgaacgat ggttttggag ttggtgacct
     7441 ggggcatgat ctcccaggtg caattcggct tggcatagat ggcttccccc aggggccgtc
     7501 caaccttccg tttgtccaca tcgaatgccg ccaccacttc caggtccccg ggttggtatc
     7561 cccccagttc aaaatgcatc aaccccagtt tctccgcacc ctcgtgggtt tggtaatatt
     7621 caataccttg aatcagggag cttgcgcaat tgcccacgcc cacaaccgcc agcctgatct
     7681 tctccattgc ttcagccctt ctttgaaaat cgaactctca ccgtctcccg ttgggaaaaa
     7741 gtaacccccg ccacccttct cgacacgggt cccggaaatc aggaatccca ggttacggcc
     7801 atattgccct cccgagcatc gatggtaaac cgagaatttt cttgccagtg cagggtgatg
     7861 gggatcttca cctgttcatc aatccgcctt ttcaagaaac gggcgccgta tttgagactg
     7921 aacccgctct cggccagaat tttcagcgcc gtttcagaaa attgcaggga ttttccgtaa
     7981 gggacgaggc tcctgtggac cgccgcgaga tagcgccggg cgatctcttc cacttcttct
     8041 tgagtcaaag gagaaaagac tacaatatca tcgatgcggt tgagaaattc cggggagaaa
     8101 tgactctccg cctctttcat caccgccttc ttgaccgtgt ccagatggtg ggaatccacc
     8161 aggaagccca tgggcttggt gaaccgttta aactcatccg atcccaggtt ggaggtcatg
     8221 atgaggaccg tatcgctgaa atacaccttc cggccgcgcc cgtcggtcag aaagccctca
     8281 tcgaaaacct ggaggaaaag gttgagcacg tccgggtgag ccttctccac ctcatccaga
     8341 aggactaccg tgtaggggtt atcccggatc tggttggtca ggacccctcc cctctccgag
     8401 cctacaatcc cccggggcat gccgatgagc ttatccacgg agatggcgga atcccggtac
     8461 tcggacatgt ccagccggac catctttctc tcttcgccga agagaaactc agccagggac
     8521 ttgcccaatt ccgtctttcc cactcccgtc ggccccagga agagaagaac tccgtccggt
     8581 cgttcgaacc tttctttcaa agggccttta ttcaggcgca ggttccgggc cacagccgaa
     8641 atggcctccc tctgccccac cacccggtgg cccagggcct cctccaggtc tttgaagcga
     8701 tgggttgtgt cccggaagac catctcctgg ggaatccgcg tctcctggga gatgatctgg
     8761 atgacatcct gcgggcccac cggccgcggc aggtcttcca tctccacccg gaccgaggcg
     8821 gtgtccagcc agttgatggc tttatcagga aggcgcaggt tgtgctggta acggggagcc
     8881 atggacaggg cggtttcgat cgcttcatcg gcaatctgaa cggaatagtt cttttccagg
     8941 cggggccgga tcccccgcaa aatctcccgc gcttcattaa ccgagggctc cagtacggtt
     9001 acgatgcgga acctccgggc cagggcctca tcctcggcaa tatgggtttt atactcactg
     9061 gccgtagtgg cgccgatgat ctggacctct ccccgggcca aggaggactt gaagatggtg
     9121 gccgcgtccg tgggtacgcc catggccgtt ccggccccaa tgagggtatg ggcttcatct
     9181 acaaagagga tgatgttgtg ccgccgcttc acctctttga ttacgttctc gatccggtct
     9241 tcaaacatcc cccggaacat ggtcccggcc actaacgtgt tcatctggag gttgacgatt
     9301 tggaagttcc tcaaccgctt ggggacggaa tcgggagaga gttccagcct ggtggccagg
     9361 ccctccacga gggcggtctt tcccactcca ggctctccga ggatcatgac cgagttgggc
     9421 cgatcccggt ggcagaggat ctccatcatc tgccggattt ctccatcccg cccgataatc
     9481 ggaggaagtt tgcccaaacg ggccagtttg ttcaggttta cgccgaaatt gttcaggtaa
     9541 gggggaaggt cgtagcgctt tttgaaatcc tcgctctttt cgtcaatcac tttggtctct
     9601 acggagatca gacgtgtggc ggtattgggt tccaccccca ttccccgcag gatgcggacc
     9661 gggatgttct gcccttcctg gaagatggcc ttaaggaggt cggtggtttc gatcaccttg
     9721 cggccggagt tgatcgctga atcccaggct agcttgaaga tggctttggt ggaaggagga
     9781 accttcattc cccctcccca aacctgtttc gaggagttta ggtactcccg tagggagaga
     9841 accacttccc gggggctgaa attcagcttt tcggcgattt cggcgaagag gctgctttcc
     9901 acctcggtaa tggccaaaaa gatgtgttcc acccccaggt aatgatgctg tcgcttctgc
     9961 gattcatgaa tcgccgaaga cagaaccttg tgggcctttt ccgaaagata atccttataa
    10021 ttacttagat cgatcataaa aaattcggag atagaaactc tacttttccc ttccgggctt
    10081 ccgcggccgg ccccttcggt tggcggcatc gaccaatagg tgacgccgca attcttccaa
    10141 taattccctc gattttttat ccacccgccg gggaacccaa atttttgggg aaatcaccag
    10201 gtctccccgt ccacttccac cgagcaatgg gatgccccct tctgggactc gaatcgcctc
    10261 tccgctctgg gttccagccg gaaccttgac tttgattttt cctccgagag tgggaacctc
    10321 gacttctgct ccgagggcag cttcccacac ggccaaagga agctcgaggt gcaggtcctc
    10381 cccctttcgc tccaaaactg ggtgtttctt cacccggact tcggcaacca ggtctcccct
    10441 tggacccccg cgtctcgcct tatttcccat tcgggagaag taaaggcgac agtgatcatc
    10501 cactccgggc ggtacccgaa tggggaccca tatgcgctgt tggatccggc cttttccgga
    10561 gcaggcctcg caacgctgga gggagacttt cccggtcccc cggcaacggt cgcatccgat
    10621 gcaaaaagcc gagggaggaa ggcccacctg aatctgcccg gcaccgccgc aaaggctgca
    10681 taatttctgg ggccctcccg caaaaaatcc ctctccctgg cagacagaac agggattctc
    10741 ctttagaatt tgaaccttct tcaccactcc ccggacggcg ctggccaggt cgatttccag
    10801 ggtgaagtga atatccctcc ctctttctac accctggaaa acccccggat ttcccccttt
    10861 ggacccttgc tcccagaaat cctcaaaccc ttccaggctg ttctcttcgt ccccttcgtc
    10921 cagccccact tcccagaagc cttcctgaaa gggaacatca agtcctaacc gatcgtatct
    10981 ttcccttctg ttcttatcgg aaagtgtttc atacgcctcc aaaatctctt tgaagcgaaa
    11041 atcagtagtc ttgttgcccg gatggggagc ggtctggtag gtgcgggcta gcttttggta
    11101 tgctttctca atctcttctt cggttgcgcc tctccgaatt cccaaaatgt cgtaataatc
    11161 ttttctggat gccattgata aaaaacccgg tcattctacc ggagaatgac ttacccggtt
    11221 cttatatacc ataagccggc tcttcttttc aacacaaaag atggtagggg aaaaagaccc
    11281 gtagggggcc tctaaacaag caataagtat tcccgcaatg gaaagaggct ttggaaatga
    11341 ttcagaaaaa aaaggggggg atcctttcga tccccccctt aggaagggtt acttcttctc
    11401 ttcgaattct gcgtccacca cgtcctcatc tggcttgcca gcctggggac ctgtgggtcc
    11461 tgccccgggt ttctgcttgg aagcggtctg ctggtacatg atctcggcca ggcgatggga
    11521 ggctttggtc aagagctcca ttttctcccg aatccgattt gtctccccgc cctgaatggc
    11581 ttctttagct tccttgagtg ccgcttcgat ggcgcctacc tcattgaccg ggattttatc
    11641 ccggtgttcg ctgagagttt tctcggtctg atagaccagg gtgtcagcct gattcctcaa
    11701 ttccacttcc tgccggcgac gtttgtcatc ggcggtatgg gcctcagcgt cttttaccat
    11761 cttttcaact tcatccttgt tcaaaccgct ggatgcggta atggtgatgg cttgctgttt
    11821 ccccgtggca ttgtccttgg cggagacatt gaggatgccg ttggcatcga tgtcgaaagt
    11881 cacttcgatc tgagggatcc cccggggagc cgagggaatt cccatgagat ggaatttgcc
    11941 cagagtccgg ttgtcccggg ccatatcccg ctctccctgg agaacatgaa tctccacgct
    12001 ggtctggctg tccgccgcgg tggagaagat ttcgctcttc cgagtgggaa tcgtggtatt
    12061 gcgatcgatc agccgggtca tgaccccgcc cagagtctcg attcccagag aaaggggggt
    12121 cacatccagg agaagaacgt ccttcacctc tccccccaga actccggcct gaacagcagc
    12181 gcccgcggcc accacttcgt ccgggttcac gcccttgtgg ggctctttac cgaagaactg
    12241 gcggaccagc tcctggactt tgggaatccg ggtggagcca cccactagaa cgacctcgtc
    12301 gatctgactg gttttgatcc ccgcatcctc cagggcctgc ttgcagggag gaagggattt
    12361 ttccaggagg tccatgacca gggactcgaa cttcccccgg gtcagcttga tgttcatgtg
    12421 cttggggccg gatgcatcgg cggtgatgaa gggaagattg atctccgtct ccatggtgga
    12481 ggagagctcg atcttggcct tctccgctcc ctccttcagc cgctgcaggg ccatgaggtc
    12541 cttgctgagg tcgatcccct ggtctttctt gaattccgcc gccaggaagt cgatgatcct
    12601 ctgatccagg ttgtccccac ccaggtgggt gtccccgttg gtggatttga cctccaccac
    12661 gttgtccccg acttccagaa tggagatgtc gaatgttcct cccccgaagt cgtaaaccgc
    12721 tatggtctcg tctttcttct tgtccagacc ataggccagg gccgcggccg tgggctcatt
    12781 gatgatccgc cggacattca gcccggcgat gaccccggcg tccttagtgg cctggcgctg
    12841 gctgtcgttg aaataggcgg gaacggtgat gaccgcatcg gtcaccttct cacccaggta
    12901 atcctcggcc gacttcctca gcttctggag gatcagggcc gagatctccg gcggggagta
    12961 ttccttgtcc ttgatcttca cccgggccgc gccgctccca tcctggatta ccttgtaggg
    13021 aaccattttc atctcttcgt tcacttcatt gtagcgacgg cccatgaaac gcttgatgga
    13081 atagaccgta ttttccgggt tggtgaccgc ctgcctcttg gccacctgac ccaccagacg
    13141 gtttccgtcc ttggtaaagg ccaccaccga aggggtcaac cggcttccct cttcgttaat
    13201 gatgaccttg gggtcgcccc cttccatcac ggcaacgacg gaattcgtcg ttcctagatc
    13261 aattccgatg attttggcca ttttattttc ctcccccagt tatcctcttg tagggtatat
    13321 ttgaaaaatc aaaaatctaa gttttaccta aacttaatca ctggattggt tcctgtcaag
    13381 gaaacctcgg cccattttcc cccggaatcc agttccttgc ccgtttagcc ggcccggact
    13441 gttaaagcac ccccaattca gcggcgttca ggtctcaagc acgggtccct gcagcccggg
    13501 atcgcggttt ggccgaaggt ttggaaaaaa ccagtccatt tttctgggtt gcatcgactt
    13561 caacggtatc cccctcggag aattctccct ccagaatctt gagcgccagt gggtcctgga
    13621 tgaagcgctg aatcgcccgt ttaagaggcc gggctccgta aatcgggtcg aacccttcct
    13681 tggccaggaa ctctttggcc cgctccgtca gggtcataga gatcttccgc tccgctaggc
    13741 gtttcctcag gttccgtagc tggatctcga cgatttcttt aatattttca agagtgagcg
    13801 cgtggaaaat aataacctca tccactcggt tcaggaattc aggcttaaaa tgagcccgca
    13861 gcgcctcgag cacccttcgt ctcatctctt catagtcccg ggcctcctga tcctggatcc
    13921 actggcttcc aatgttggag gtcataatga tcaccgtgtt cttgaagtct accgtgcgcc
    13981 ctttcccgtc ggtaagccgt ccgtcgtcaa ggatttggag gagagcgttg aagacctccg
    14041 gatgggcctt ttccacctca tcgaagagga ctacggaata gggtttccgt cgcaccgcct
    14101 cagtcaggta tcccccttct tcgtacccca cataacccgg gggtgcaccg atcagccggg
    14161 caacggagtg tttctccatg aactcggaca tatccagacg aaccatggcc tgttcatcgt
    14221 caaatagaaa ttccgctaag gcccgggcca gttcggtttt tccgaccccg gtgggcccca
    14281 ggaagataaa ggatccgatg gggcgattgg ggtcctgcag gtcggcccgg gcccggcgga
    14341 cggagttgga aaccaggcga atcgcctctt cctggcccac tacacgattc ttcaagcggg
    14401 attccatctt gaggagtttt tcgatctccc cctctatcat cttggaaacg gggatccccg
    14461 tccacttggc caccacttcg gccacgtctt cggcatccac ctcctccttg agcatctttc
    14521 gctctttctg cagggccgcc agggcctgat tttccctctc caggtttttt tggaattcac
    14581 tggccttgcc gtatcgaatt tccgctacct tggccaggtt cccctccctt tcaaattgct
    14641 gctcctgccg cttggcttct tccatccttt ccttcagggt acgaatcttc tggatggcct
    14701 ctttctcctt ctgccagtgg gccttcatgc cttcgctctg gcttctgagc tcggccagct
    14761 cttggtcgat tttctccagc cgctccaggg aggacttgtc tttctccttc agcaaggcct
    14821 gtcgctcgat ctccacctgg gtgatcttcc gctccacctc gtccacctcc acgggcatgc
    14881 tgtcgatttc gatcctcagc cgggaagcgg cctcatcgat caggtcgatg gctttgtcgg
    14941 gaaggaaacg atcggatatg taccggtggg acagggtcgc cgcggccacg atggccgagt
    15001 cctgaatccg gactccatgg tgcacttcgt agcgctcctt cagcccgcga agaatggcga
    15061 tggtatcttc cactgtgggc tcggagacca tcaccggctg aaatcgccgc tccagggcgg
    15121 catccttttc cacatgctta cgatactcat tgatggtcgt tgccccgacg caccgcaatt
    15181 ccccccgggc caaggccggt ttcagcatat tggaggcgtc catggccccc tccgccgcac
    15241 ccgcccccac cagggtgtgc agctcatcga tgaagagaat gatttcccct tccgcctcgg
    15301 tgatctcctt gaggagggcc ttcaggcggt cttcgaactc tccccgatac ttagtcccgg
    15361 cgatcagggc tcccaaatcc agggccacga gttttttatt cttcagggtt tccggcacgt
    15421 ccccgttgac gatccgttgg gccaggcctt ccacaatggc cgttttcccc actccgggct
    15481 ctccgatcaa gaccgggttg tttttcgtcc tgcgggaaag gacttggagg accctgcgga
    15541 tttcgtcgtc ccggccgatg accgggtcga gtttgccctt gcgggccaag tcggtcaaat
    15601 cccgggtgta acgggtcagc gcctggtatt tctcttctgg gctctggtcg gttacccgct
    15661 gggaaccccg gatcgacatg aggactttat agacggcatc cttgctggct cccaggccga
    15721 gcaggatacg ggaggccgac ccatctcgct cttcaataat ggccagaagc atatgctccg
    15781 tgctcacgta tgcatctttg agccgctcgg cctcctcgaa ggagctgtcc atcacttttt
    15841 tcaggcgggg cgaaatatat acctgacccg ctcccccgcc atagactttg gcaatcttgt
    15901 ccaaggcttc ctcgacttgg gtggtaaggg cgggaatgga aactccaagc ttctgaaaga
    15961 cttgaggaat aattccttcg gtttggagaa tcagggccag caggaggtgc tcgggttcca
    16021 cttgctggtg ctcatgcttt tcggttatgg cctgggcctc ttgcaaggcc tcctgggctt
    16081 tcagggttaa tttgtcgaat ctcatcttct ttcctttctt cagtcaaaat aagggtctcc
    16141 aaaatcatgg gaggaggaat agatgatagc gaagtcaact tttccgtaga aaaaaaaggg
    16201 gggctttcgc cccccctcaa ggattgggtg tttagccaac gtccactttg atctgtttcg
    16261 gcctggcctc ttccgccttg ggaatcgtga cctccagaat gccgtctctg aagcgggctt
    16321 ttactttctc ctgttgcacc gtactgggca gggtgaaagc ccgcatgaag gagccgtagg
    16381 tccgctccat acgatggtag ctttcttcct tcacatcctt ttccagcttt ttctcgccct
    16441 tgagagtcag ggtgttatcc ttgacttcaa gggtcaaatc ttctttcgtc acccccggaa
    16501 gctccacgtt aagaacgatc gcttccttgg tttcgtaaat atccaccgct ggaacccaac
    16561 cgccctgagc catttcttcc tccgtccacg gagtacgggt ccggaagtca gacatcaaac
    16621 ggttcattct ttcctgcaat gccgacattt cccggaaggg atcccatcga atcatcgcca
    16681 tcgttttatc tcctttccta tggattgaaa cttttttatc gattcatcca aaaagtaagc
    16741 atggaattcc tccttgtcaa ccccgtggag gcacgagttg tttcgcgggg ttgaccgtgg
    16801 cctattcaag gaggcaaaga aggaatcgag ggggtgggga aggaaacgga ggggagagaa
    16861 agggaaccct cagagttctt ttttctcttg acccagcttc ttgaaccaga cctggatgtc
    16921 ctccaggtct gggaactgtc cctgcaagaa ggccctcagc ttgtccgcaa cttttttttc
    16981 aatctgacgg gcccgctccc gggagacctt tagctgggtt ccggcttcct gcaaggtcat
    17041 gggttcatcg gccatgagac ggtttttcag gaggaaggcc tctttgggtt taagcctgag
    17101 ggtgaactct tcgatctttt tcccaagaat ttcattgaac tgtgtaccgg taacggtctc
    17161 ctccggcgta tctcccccag ggaggacatc catgtaagtc tggcggccat tatcttccag
    17221 gggagtgttg agcgagaggt ccccaccttc cagccgctgg tccatttgcg tgacctcttc
    17281 cttggtcaca ttcaaggctc cggccaggag cttcggtccc gggtcaaacc ccatggcttc
    17341 caggcgacct ttctccttct tcaagcggaa gaagagcttc cgctgggcct gggtcgtccc
    17401 gatctttacc aggctccaat tgtcgatcag gaatttcagg atgtaggctc ggatccacca
    17461 ggaagcatag gaggagagct tcacctcccg gtagggatca aatttcttca ccgcctgcat
    17521 gaggcccagg tttccttcct ggatcaggtc caggaggttg ctgtaggctc gctggtattc
    17581 gaaggcaatt ttcaccacca gacgcaaatt ggaaaccacc agccgcaggg ctgcgctgcg
    17641 gtccttctcc tccctccact tctgggccag ggccctttcc tcctctcggg tgaggagggg
    17701 gaattggcga atttccagta agtacctctt taaggggtca aagacctcaa tggcggccga
    17761 tttttcggcc accaccaaat ctttcgcctc ggacggcggc aggtccaaag ggacaaagag
    17821 gtccttttcc agatcaggtt cgctcattgt actcccaccg tgaggtagcc tgtctctccc
    17881 gtccgccaat cccgcaccag gagcagggcc tcttgccccg gacggagttg ttccacgatt
    17941 cgatgatagt ccttgagccc ccggagagtt tgctgattaa tctgatagac gatatctcca
    18001 gcctccaggc cagcctgtgc agccgggccc tgcggatcga cccgggcgac cagaactccg
    18061 tcgcgggagt tcaaacgcct tttgcgcgcc tctaagatgg aaatctcttt cacttccatc
    18121 cccagtctgc tttccagttc caacgccgcc atacggggcg aaggcaggta ggcctccacc
    18181 acgggttgga cggtgattct ttcccccttg cgcaggagga cgacttccac cctcttcccc
    18241 acccgggtcc cggcaaggcg gttgcgcagc tcgttggcgt tttcaatggc ccccccgtcc
    18301 aggttcacga ttacgtctcc ccggcggata ccagcctttt cggctggccc acccttggtc
    18361 acctcggcca ccagcgctcc tttgggtgat ttcaggttca gactcctggc caaagaaggg
    18421 gtcacctctt ggagactcat tcccaaccag ccgcgggtca cttttccggt tttgatcaag
    18481 gactcggcca cggccgtagc catgttgctg gggatggcga acccgattcc ttcaaaccct
    18541 ccggattcgg agacgatggc agcattgact ccgatgacct ccccccatag gttgagtagc
    18601 ggaccaccgc tgtttcccgg gttgatggcc gcgtcggttt ggatgaaatc ctgatagctg
    18661 gaagggtcta atatccccgc gcggtgcttg gcgctgatga ttccggcagt aactgtctgt
    18721 tccaacccgc gcgggttgcc gatggccacc acccattctc ccactcgcag tttgtccgag
    18781 tcgccgaatt tcaggaaagg ggtattaggc ggaagcttga tctggatgac cgccaagtcg
    18841 gtcttggggt ccacccccac aatcttggcc tccatctctt cccccgaggc gatcttaacc
    18901 tggactttcg tggccccatt gaccacgtgg ttgttagtta taatatgtcc attgccgtcg
    18961 atgatcatcc ccgtcccgat tccccgcacc tctctccggt
//
LOCUS       DASPRR010000445        10668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_915579, whole genome shotgun sequence.
ACCESSION   DASPRR010000445 DASPRR010000000
VERSION     DASPRR010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915579"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VEL68_16370"
     CDS             <1..726
                     /locus_tag="VEL68_16370"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HXZ37598.1"
                     /translation="ERLLQQGRAQRFEEQIKEWLKEFEGKPETRQVLEEMEELLRGLR
                     PDSQEVMEQEQKGKFPPLSSRQEDLKERTRALKEKLDMIAQLFPGMDTEVLKNLEGAA
                     DSMGEASGKLKGEDAPGAIPPEQEVLRRLNQSQQAMRQMSQQMAMRGQINRWGRPWGY
                     DPRPGWYYGPWAPLPTLPQPEVKRPIERGYTGIDREEFETPSKDAYQVPKTFREKIME
                     ALKENPPSPYKREVEQYFRGLSE"
     gene            723..2288
                     /locus_tag="VEL68_16375"
     CDS             723..2288
                     /locus_tag="VEL68_16375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ37599.1"
                     /translation="MKKMGFTLILSVLLMMPSAGHPVSPQVLASLEEKVERWEVEEAW
                     KEAEGLLRTESRDPQVLETAAQIAFHRGEYGSARNLIQSALEIGGEDEKRRAFSLFVE
                     STLGVLSSFQKYESPHFIIHLDEKQDGILVDYLRDNLERTYRVMAERYGFQPKEKIRV
                     EVFPDTRAFYLCSTLSARDIEVAGAVGLAQFNKLLILSPRALVYGYRWMDSLCHEYLH
                     YLIMKLTANRAPIWFHEGLAKYEETRWRGGPSYLNLLSQTLLAQALSKGKLIEWEKME
                     NSFIKLETPEEVQLAYAQAASVIDFILSRAGDPGLREIMRRMGDSSSPEGAREAIQQV
                     LGWPFLEFSEKWKESLAKKDLKEMDGVHARRYKIKEGKSDEERVEMNEIKSMIARNRA
                     HLGDRLKERGRMEAAVLEYHRALAESRESVPVMNRLSDALIRMNRAPEALKVLKEAEA
                     LAPDHPTIYSYMGKAFLKIQDFPSARGALEESIQINPFNPEVHQNLALAAERMGDSDT
                     ALREREILRRLVR"
     gene            2297..3310
                     /locus_tag="VEL68_16380"
     CDS             2297..3310
                     /locus_tag="VEL68_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXZ37600.1"
                     /translation="METAPELQITDHDLSQVEEMGRKKADIVQEVHKVIIGQDKVIEE
                     VLIALFCRGHCLLVGVPGLAKTLLISTLAGIMELKFNRIQFTPDLMPSDITGTDILEE
                     EAVSKRRSFRFLKGPIFTNILLADEINRTPPKTQAALLQAMQEYRVTAGGVTHALDLP
                     FFVLATQNPIEQEGTYPLPEAQLDRFMFNISIEYPALEQEMEIVAATTSSYQPALQKV
                     ITGEKILEMQDLVRKVPASSHVIGYAVKLLRATRPREPEALPFVKDWVEWGAGPRASQ
                     HLILAAKAKAIMDGRYVASVEDIKSVIRPILKHRIITNFKAQAESVTSLEVIDHVLKE
                     VSP"
     gene            3307..4194
                     /locus_tag="VEL68_16385"
     CDS             3307..4194
                     /locus_tag="VEL68_16385"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXZ37601.1"
                     /translation="MNLFDPRVLTRIKNLSLRARSVVDGVMVGIHPSRAKGFSSEFEG
                     HREYAPGDEVQHIDWKTFGKFDRYFIKEYQETTHLRAYIVLDRSASMGYASDGYSKFD
                     YGATLAASLSYLLLKQQDAVGLILFSRRIERMIPPKAAPGHFWAILKALEGQKPEGET
                     SGGDVLQELAGSLRRRGLIILISDLLDDPLKVLRGLKQMRSRGSDVLVFHLLDRDELE
                     FPFDQPAVFKDLEEDLRLPADPQVIRSAYLQTLRSLMQTYEKTCASYQIDYHLFPTSV
                     GLDKALTKYLTWRKNFKPR"
     gene            4515..6494
                     /locus_tag="VEL68_16390"
     CDS             4515..6494
                     /locus_tag="VEL68_16390"
                     /inference="COORDINATES: protein motif:HMM:NF019220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HXZ37602.1"
                     /translation="MSITFLNPLFLFGLAAGVIPILIHRLVQRHGKVKKFSAVRLLLL
                     SQQNLVRPERLKEFILLALRVLAVMSLALLVARPVWLFPGVFSSGEEGAKVLILDNSL
                     SMGYREDRGERFELARTHAREILNTLKGRVLILPTASVPGKILDEHPVEWMNSEQALR
                     ELDTISLSFGKGDPGAALNRAFQAGKDLKAPLEVVILTDLARGEWEGLQQGQLKNVPS
                     NLTLTFVRFGGIERDPNLTVKEVRLSLGEAVVGAPSRLEVTVGNLSSQAASAQVQLIL
                     NSAKVEQKSIDLPSGEDGKLSFDLTPDKPGWVRGEVRLAGDRLPLDDRFYFPVKARER
                     VKVLVVDGDPRTAFKASESYYLVNALQPGRGEGTPFSVQVIQEGELERMDLKSYEAIF
                     LLNVPQPPPSPLAAYLQAGKPLFIFLGNRVNPEQYQRLPFFTWQLKEVKEGGVSARLL
                     PGPGSLSREGFTSLSGISGESLKKASFERYYRVVKTGKTLLMLENGDPLLLEGEWGRG
                     HIFLFASSADLDWNDLPLSAAFLPLIHGLLKEAVGLSQSPLPPTLRVGESFPGTAPPA
                     QIAGIPGGPGIYQFSAASGDEWRGLNPPVEESDLRKMTAEEMKKRLVSMEVKVIESRE
                     GSAGTPLAGRKDLWPYILGFLLAILGIEMGVAGRI"
     gene            6491..7252
                     /locus_tag="VEL68_16395"
     CDS             6491..7252
                     /locus_tag="VEL68_16395"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HXZ37603.1"
                     /translation="MISRREFLRSALALGGGVLFVPKHLYGENSFPHFFFTQIRYRGG
                     AWDPNPQFVEAMVEELELRTSIDGARERRAIELSDPDLFYSPFLFMAGKYEFEPLTPQ
                     EREILRRYLTFGGFLFAEDTLGVKGYGFDRAFRAEIKRVFPGQELKRLPLDHPVYQSF
                     YLINQIGGRQPISPYFEGVILDQWTPVIYSQNDLSGAWSRDKLGKWVYECTPGGEAQR
                     SLAFKAGINVIVYSLTSDYKKDLVHHPFIKKRLNP"
     gene            7249..9435
                     /locus_tag="VEL68_16400"
     CDS             7249..9435
                     /locus_tag="VEL68_16400"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HXZ37604.1"
                     /translation="MTWKDITLSPLLPLWLILALLGLGVLALILQYPRLRTRVGRSRC
                     LWLSALRLGALLLLIGCFLDPASTERVEKKSSPTLALFVDTSSTMALPGRGGKSRLEE
                     GIALLLGGEKPLLKSLSERFEVQVYALGESTRPVEAGEVSKLKPAGSRGDPAAAVEKL
                     SSRNVLPVLLSDGRVKWEGKGSLRLPFFTVPVGDPGDYRDIWIETLKAPAVAFRGREV
                     RVEAVVKSHGFRALTLPVSLRDGQKLLTAQSIRIQGDSAEIPLSLSFTLEDVGPHHLS
                     LSVPPQAGEGSALNNSFPFSLRVARDKVRILMISGHPSLNYRYLRMAFKSDPSIDLLS
                     FVILRTPTDVLNVPLQEQSLIPFPVETLFSKELPQFDLLVLDDFSFHLYVKPAYLEKV
                     REFVKAGGGLAMIGGPNLLDGGKYSGTPMEDVLPVSTAGKGEYQREIAAKVKLTRSGG
                     SHPITRLEATEKENLDLWKEMPALDGINRVQMKTSATALLEGGDGVPSPIVSVGKYGQ
                     GRVLVLATDYSWKWYMGMVGRGRGQWAYHRFVERTVRWLTRDPGLDPIQVLLPEDRFR
                     RGQKKEVRVRVQSQEESPETQGAVHFSVLDPMGVKIGAEFKISGRPGEYLASFAPEKE
                     GTYKLKVETAGGSVEESVEISEPIEERDGLPDHERLRMIAESTGGKFISSKEDLLPEL
                     LSAVGKRESRYLEERRTPLWAHFSLPALILALLSAEWYFRRRWGLI"
     gene            9599..10396
                     /locus_tag="VEL68_16405"
     CDS             9599..10396
                     /locus_tag="VEL68_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ37605.1"
                     /translation="MKKSLTLTLFLILFLLPLWVHSACRNPYLAGEGPLIEPAAASGK
                     VEIKWFGHSFFQITSSSGTVIITDPFGAMGFPLPEVWAHVVTVGREHGNHNNVGLAKG
                     KPVILRGLKEGKDEWDTVNYTFRDVLIYNIPVHQRGFADYSLSMKGSAFVFEMDGLCI
                     LHSGDISEPYNEDQLQLIGHVDILLQVIGGTFTIGPEGALQIIDKLKPKIVIPMHFWY
                     NMGQLEKFIEGPLPARRLDTNTITVSKETLPKETEILILKVSREGDL"
BASE COUNT         2304 a         3004 c         3166 g         2194 t
ORIGIN      
        1 gaacggcttt tgcagcaggg gcgggcccag aggttcgagg agcagatcaa agagtggttg
       61 aaggaatttg aggggaagcc cgagacgcgc caggtcctgg aagagatgga agaactgctc
      121 cggggtctgc gccccgattc gcaggaggtc atggagcagg agcaaaaagg aaaattcccc
      181 cccctctctt cccgccagga agacctgaaa gaaaggaccc gggccctgaa agagaagctg
      241 gacatgatcg ctcagctctt cccgggcatg gacacggagg tcttgaagaa cctcgaagga
      301 gcggcggact ccatgggtga ggcctcgggc aagctgaagg gcgaggatgc tcccggagcc
      361 atccctccgg agcaggaagt tctccgtcgg ttgaatcaat cccagcaggc catgcggcag
      421 atgtcccagc agatggccat gcgcggacag atcaaccgtt gggggcggcc ctggggatac
      481 gatccccgac ccggctggta ttatggtcca tgggccccgc tgcccacgct tccccagccg
      541 gaggtgaaac ggccgatcga gagaggatac acggggatcg accgggagga gtttgaaact
      601 ccctccaaag acgcctacca ggttcccaag acctttcgcg agaagatcat ggaagcgctg
      661 aaagagaatc ccccctctcc ctacaagcgc gaggtggagc aatatttcag gggtttgagc
      721 gaatgaaaaa aatgggcttt accctgattc tatcggtcct gctgatgatg ccctctgcgg
      781 ggcaccctgt atctccccag gttctcgctt cgctggagga gaaggtggaa cggtgggagg
      841 tggaagaagc ctggaaggaa gcggaagggc tcctgaggac cgagagccgg gatccccagg
      901 tcctggagac ggccgcccag atcgccttcc atcgggggga atacgggtcg gccaggaatc
      961 tcatccagtc ggctctggaa atcggagggg aagacgaaaa gcggagggcc ttttccctct
     1021 tcgtcgaatc gacgctgggg gtgctgtcgt cttttcaaaa atatgagagc ccccatttca
     1081 tcatccacct ggacgagaaa caggacggaa ttctggtgga ttatctccgg gacaacctgg
     1141 agcggaccta ccgggtcatg gccgaacggt acggcttcca gccgaaagaa aaaatccgcg
     1201 tggaggtctt cccggacacc agggccttct atctgtgctc cactctttcc gcgcgggaca
     1261 tcgaagtggc gggagcggta ggcctggccc agtttaacaa gctgttgatt ctctctcccc
     1321 gggctctggt gtacggatac cgctggatgg attctctctg ccacgagtac cttcattacc
     1381 tgatcatgaa gctcacggcc aacagagccc ccatctggtt tcatgaaggg ttggccaaat
     1441 acgaagagac gcggtggcgc ggaggaccct cctacttgaa tctcctctcg cagacccttc
     1501 tggctcaagc tctgtccaag gggaagctca tcgaatggga gaagatggag aactccttca
     1561 tcaagctgga gacccccgag gaagtgcagc tggcctacgc ccaggcggcc tcggtcattg
     1621 atttcattct ttcccgggcc ggagacccgg ggcttcggga gatcatgcgc cgaatggggg
     1681 acagcagttc tccggaaggg gcgagggaag ccatccagca ggtgctggga tggcccttcc
     1741 tcgagttcag cgaaaaatgg aaggagtcat tggcgaagaa ggacctgaag gagatggacg
     1801 gcgtccacgc gcggcgctac aagatcaaag aggggaagtc cgacgaagaa cgggtggaga
     1861 tgaacgagat caaatccatg atcgcccgga accgcgccca cctgggcgac cggctcaagg
     1921 agcgggggcg gatggaagca gcggtcttgg agtaccaccg ggccctggcg gaaagccggg
     1981 agtcggttcc ggtgatgaac cggctctcgg acgcgcttat ccgcatgaac cgggcaccgg
     2041 aagcgctgaa ggtgttaaag gaggccgagg ccctcgcgcc ggaccatccc acgatctaca
     2101 gctacatggg taaagctttt ctcaagatac aggattttcc ctcggcccgg ggggccctgg
     2161 aagaatccat ccagatcaat cccttcaacc cggaggtcca tcagaacctg gccctggcgg
     2221 cggaaaggat gggagattcc gacaccgccc tgcgggaaag ggagattttg cgcagactcg
     2281 ttcggtgagg aagagtatgg aaaccgcacc agagctccag ataaccgatc atgacctttc
     2341 ccaggtcgag gaaatgggcc gcaagaaggc cgatatcgtg caggaggtcc acaaggtgat
     2401 catcggccag gataaggtga tcgaagaggt cctgatcgcc ctcttctgcc gcggccactg
     2461 tctcctggtg ggagtgcccg gcctggccaa gaccctgctc atctctaccc tggccgggat
     2521 catggaactc aagttcaacc gaatccagtt caccccggac ctcatgccct cggacatcac
     2581 cgggacggat atcctggaag aagaggcggt cagcaaacgt cgttccttcc gctttctgaa
     2641 gggccccatt ttcaccaaca tcctcctggc cgatgagatc aaccgcactc ctcccaagac
     2701 ccaggccgcc cttctccagg ccatgcagga gtaccgggtt accgccggag gcgttaccca
     2761 tgccctggac ctccctttct ttgtcctggc cacccagaac cccatcgagc aggagggaac
     2821 ctatcccctg ccggaggctc agctcgaccg gttcatgttc aacatctcca tcgaatatcc
     2881 agccctggaa caagagatgg agatcgtggc cgccacaacc tcttcgtacc agccggccct
     2941 gcaaaaggtg atcaccgggg aaaagattct ggagatgcag gacctcgtcc ggaaggtccc
     3001 cgcttcttcc catgtgatcg gctacgcggt caagcttttg agggcgactc ggccccggga
     3061 acccgaagcc cttcctttcg taaaggactg ggtggagtgg ggggcagggc cccgggcttc
     3121 ccagcacctg atcctggcgg ccaaggccaa ggcgatcatg gacgggcgct acgtggcttc
     3181 ggtggaagac atcaagtcgg tcatccgccc catcctcaaa caccggatca tcaccaactt
     3241 caaggctcag gccgagagtg ttacctcgct ggaggtcatc gaccacgtgc tgaaagaggt
     3301 cagcccgtga accttttcga cccccgggtg ctcacccgaa tcaagaacct ttccctccgc
     3361 gcccgatcgg tggtggacgg ggtcatggtg ggcattcatc cttcgcgggc caaggggttc
     3421 agctcggagt tcgagggaca ccgggagtac gcgcccgggg atgaagtcca gcacatcgac
     3481 tggaagacct tcgggaaatt cgatcggtat ttcatcaagg agtatcaaga gaccacccac
     3541 ctgcgagcct acatcgtcct ggacaggagc gcgtccatgg gctacgcctc ggacgggtat
     3601 agcaaattcg attacggcgc taccctggcc gcgtcccttt cgtacctgct cctgaaacaa
     3661 caagatgcgg tggggctgat tctcttctcc cgcaggatcg agagaatgat cccgcccaag
     3721 gccgctcccg gacacttctg ggccatcctg aaggcgctgg agggacagaa gcccgagggt
     3781 gagacttcgg ggggggatgt gctgcaggag ctggccggct ctttgaggcg gaggggactg
     3841 attattctca tctccgacct cctggatgat ccgttaaagg ttttacgggg gttgaagcag
     3901 atgcgctccc gggggagcga tgttctggtc tttcacctgt tggaccggga cgagctggag
     3961 ttccccttcg accagccagc cgttttcaag gacctggaag aagacctccg cctgccggct
     4021 gatccccagg tgatccggtc ggcctatctg cagaccctcc gctccctcat gcagacctat
     4081 gaaaaaacct gcgcctctta ccagatcgac taccatctct tccccacttc ggtcggcctg
     4141 gacaaagccc tgacgaagta tctgacctgg aggaagaatt tcaaaccacg atagatagga
     4201 tgaatccgaa gaacgaaatt tgaaatccga aagaaatttg agtgagcgaa atttccattt
     4261 ttcgaaacga aaaccaaaga ggtttttaca catgtttcaa cccccgtcat tccggcgaaa
     4321 gccggaatcc agaattgccg taaaaacctg gattccgggt cgcgcttcgc ttgcccggaa
     4381 tgacgacatc ccctcccttc aagagcttgc aggaaattcc aaagggccgc aatttctgcg
     4441 attggaaaat ttaggggttt ggattttcct ttggtttcgg gtttcgatat ccggatttcg
     4501 gatttgattc ctttatgagc atcacttttt taaatcccct tttcctcttc ggcctggccg
     4561 ccggcgtgat ccccattctg atccaccgcc ttgttcagcg tcatgggaaa gtgaaaaagt
     4621 tttccgccgt ccgccttctc ctgctgtccc agcaaaacct ggtccggccc gagcggctga
     4681 aggagttcat tctcctggct ctgcgggtcc tggcggtgat gagcctggcc ctgctggttg
     4741 cccggccggt ctggctcttt cccggggtct tttcctccgg ggaagaaggg gccaaggttt
     4801 tgatcctgga taactccctg agcatggggt accgggagga ccggggagag cggttcgagc
     4861 tggccagaac ccatgcccgg gaaattttaa ataccctgaa aggccgggtg ctcatccttc
     4921 ccacggcgtc cgttccgggg aagattctcg atgaacatcc cgtggagtgg atgaattcgg
     4981 agcaggccct ccgggaactg gacacgatct ccctttcctt cggcaaggga gacccggggg
     5041 ccgctttgaa ccgtgccttt caggcgggga aggatttaaa agcgcccctg gaagtggtga
     5101 tcctcactga cctggcccgg ggagaatggg aaggactgca acaagggcag ttgaaaaatg
     5161 tcccctccaa cttgactctg acctttgtcc gcttcggagg tattgagagg gaccccaatc
     5221 tcacggtcaa agaggtgcgc ttgagcctgg gagaagcagt ggtgggggct cccagccgcc
     5281 tggaagtgac cgtaggaaat ctgtccagtc aagccgcttc ggctcaggtg caactgattt
     5341 tgaacagcgc caaagtggaa cagaaatcca tcgatctccc gtcgggagag gacgggaaac
     5401 tcagctttga tctcaccccg gacaagcccg gctgggtgag gggagaagtc cgcctggccg
     5461 gcgatcgcct tcctctcgac gaccggttct atttccccgt aaaagctagg gagagagtaa
     5521 aagtcctggt cgtggatggc gatccccgga cagccttcaa ggcgagcgag agctattacc
     5581 tggtaaacgc cctccagccg gggcggggag agggaacccc cttcagcgtc caggtgattc
     5641 aggaggggga actggagcgc atggacctga agtcctacga ggccatcttc ctcctgaatg
     5701 tcccccagcc ccctccttct ccgctggctg cttatctgca ggccggcaag cccctgttta
     5761 tcttccttgg gaaccgggtg aacccggaac aatatcagcg ccttcctttt tttacctggc
     5821 agctgaagga ggtaaaggaa ggaggggtgt cggcgcggct actcccggga ccaggaagtc
     5881 tgagccggga aggctttacg tccctttccg gaatttcggg ggagagccta aagaaagcct
     5941 ccttcgaacg ctactaccgc gtagtcaaga ccggtaagac tcttttgatg ttggagaacg
     6001 gagaccccct tctgttggag ggagagtggg ggcgggggca tatcttcttg tttgcctcca
     6061 gcgccgacct ggactggaat gaccttcccc taagcgccgc cttcctgcct cttatccacg
     6121 ggctgctgaa agaagccgtg ggtttgtccc agagccctct tcctccaacc ctgcgggtgg
     6181 gtgaatcttt tccggggaca gccccgcctg cacagattgc cggaatcccg ggagggccgg
     6241 gcatatacca gttttccgcg gcctctgggg atgagtggcg gggattgaac ccgccggtgg
     6301 aagaatccga ccttaggaag atgaccgcgg aagagatgaa aaaacggttg gtatccatgg
     6361 aagtcaaggt catcgaatcc agagaaggga gcgcgggaac tcccctggcc ggtcgaaagg
     6421 atctctggcc ttatatcttg ggcttcctgc tcgcgatcct gggaatcgag atgggggtgg
     6481 ccggaaggat atgatctcca ggagagagtt cctgcgatcg gcgctggccc tgggcggtgg
     6541 tgttctcttc gtccctaagc atctctatgg ggagaactcc ttcccccact ttttcttcac
     6601 ccaaatccgc taccggggcg gagcctggga ccccaacccc cagttcgtgg aagccatggt
     6661 ggaagagctg gagttgcgga ccagcatcga tggcgcccgg gaaaggcggg ccatagagct
     6721 gtccgacccc gatctcttct actccccctt cctcttcatg gccggaaaat atgaattcga
     6781 acccctcacc ccgcaggaga gggagattct gcggagatac ctgactttcg gtggatttct
     6841 cttcgcagaa gataccttag gggtgaaagg atatggcttt gaccgggctt tccgggcgga
     6901 aataaaaagg gttttcccgg gccaggaatt gaaacgcctg ccgctggatc atccggttta
     6961 ccagagtttt tatttgatca accagatcgg gggaaggcag ccgatcagcc cttatttcga
     7021 gggagttatc ctggatcaat ggacgccggt gatctattcg cagaacgacc tttccggggc
     7081 ttggtccagg gataagttgg gcaaatgggt ctatgagtgc actccgggcg gcgaggcgca
     7141 gaggtccctg gctttcaaag ccgggatcaa cgtgatcgtc tattcattga cctccgatta
     7201 caagaaggac ctggtccacc atccgttcat caagaagcgt ctgaacccat gacctggaag
     7261 gatatcactc tatctcccct gcttcctctc tggctgatcc tggccctcct gggcctgggg
     7321 gtcctggccc tgatcctcca gtatccccgc ctccggacaa gagtggggcg cagccggtgc
     7381 ctctggctct ccgccctccg cctgggagct ttgctcttgc tcatcggctg ctttctcgac
     7441 cccgcttcca cggaaagggt ggagaagaaa tcttccccca ccctggctct tttcgtggac
     7501 acctcttcca ccatggccct gccggggaga ggcggaaaga gccggctcga agagggaata
     7561 gcccttttgc tgggcggaga gaagcccctg ttgaaatccc tctcggaaag attcgaagtc
     7621 caggtctatg cgttgggaga atccaccaga cccgtggaag caggggaggt ctccaagctg
     7681 aagcccgcag gaagccgggg ggaccctgct gcggccgtcg aaaagctgtc ttccagaaat
     7741 gttctacccg tcctcttgag cgacggaagg gtgaagtggg aagggaaggg ttctcttagg
     7801 cttccctttt tcacggtccc ggtcggggac ccgggcgatt accgggacat ctggattgaa
     7861 acgctgaaag cgccggccgt cgccttccgc ggccgggagg tcagagtcga ggcagtggtg
     7921 aagagccacg gctttcgggc gttgaccctt cccgtatcgc tccgggacgg ccagaagctt
     7981 cttaccgccc agagcatccg catccagggg gactctgctg aaatccccct gtccctttct
     8041 ttcaccctgg aagatgtggg cccgcatcat ctgtcccttt ccgttccccc gcaggcaggg
     8101 gagggcagcg ccctcaataa ttctttcccc ttttctctcc gcgtggccag ggacaaggtc
     8161 cgcattctga tgatttcagg acaccccagc ctgaattacc gttacctgcg aatggccttc
     8221 aaaagcgatc catccatcga tctcctctct ttcgttatcc tgcggacgcc tacagatgtt
     8281 ctcaatgtcc cccttcagga acaaagcctg attccctttc cggtggagac tctcttttcc
     8341 aaggagcttc cccaatttga cctcctggtc ctggatgatt tttcctttca cctgtacgtc
     8401 aagcccgcat acctagagaa ggtgagggaa ttcgtaaagg cggggggggg gctggcgatg
     8461 atcggaggtc cgaaccttct tgacggagga aagtactccg gcacccccat ggaagatgtc
     8521 cttccggttt ccacggcggg gaagggagaa tatcagaggg agatagccgc gaaggtgaaa
     8581 ctgacccgct ccggagggag ccatcccatc acccgccttg aggcgaccga gaaagagaat
     8641 ctcgaccttt ggaaagagat gccggccctc gacgggatca accgggtgca gatgaaaaca
     8701 tcggcaacag cccttctgga gggcggggat ggagttcctt ccccgattgt ctccgtggga
     8761 aaatacggcc agggacgtgt tttggttctg gccaccgact attcctggaa atggtacatg
     8821 ggaatggtgg gaaggggaag gggccaatgg gcttaccacc gatttgtgga gagaacggtt
     8881 cgatggctaa ccagagaccc cggcctggac cccatccagg tcctccttcc cgaagatcgc
     8941 ttccgaagag ggcagaaaaa ggaagtccgg gtccgggtgc agtcccagga ggaatccccc
     9001 gagacccagg gggcggttca tttctccgta ttagacccga tgggggtgaa gattggggca
     9061 gagtttaaaa tctcgggtcg gccaggggaa tatttggcct ccttcgcccc ggagaaagag
     9121 gggacctata aattgaaggt ggaaaccgcc ggcgggtctg tggaagagtc ggttgagatt
     9181 tcggagccga tagaggaacg ggacggtctt ccggatcatg agcggctcag aatgatagcc
     9241 gagtccacgg ggggaaaatt catctcttcg aaagaagacc ttctcccgga actcctgagc
     9301 gcggtgggaa agagagagag ccgctacctg gaggagagac gaactcctct ctgggcccat
     9361 ttctccctgc cggcgctgat ccttgccctt ctttcggcgg aatggtattt ccgtcgcagg
     9421 tggggactga tctgatttcg gattgcggat tggggatttc ggattgaaag acgtatgggt
     9481 gactttggga cgcgaggtgg tccgagaaga aattgaaaac gataggtgaa ttgaatggca
     9541 gtgggaagtc tttgtttctt caatccgaaa tccgcaattt ttcagtcgga ggtttcccat
     9601 gaaaaaatca ctcaccctga ccctattcct tatcctgttc ctccttcccc tatgggtcca
     9661 ctccgcctgt cgcaatccct acctggccgg agaggggcct ctcattgagc cggcggccgc
     9721 atcggggaag gtggagatca aatggttcgg ccactctttc ttccagatca cctccagctc
     9781 gggtacggtg atcataaccg accccttcgg cgccatgggc tttccgctgc ccgaggtctg
     9841 ggcccatgtg gtgaccgtgg gacgggagca cgggaaccat aataatgtgg gactggcgaa
     9901 agggaagccg gtcattctcc ggggactcaa ggaggggaag gacgaatggg ataccgttaa
     9961 ttacaccttc cgggatgtgc tcatctacaa cattcccgtt catcagagag gttttgcgga
    10021 ttacagcctg agcatgaagg ggtcggcctt tgtcttcgag atggacggac tctgcatcct
    10081 ccactccggg gacatcagcg agccctacaa cgaagaccag ctccagctta tcgggcacgt
    10141 ggacattctc ctgcaggtga ttggggggac tttcaccata ggtcccgaag gggcgctgca
    10201 gatcatcgat aagctcaaac ccaagatcgt catccccatg cacttctggt acaacatggg
    10261 ccagctggaa aaattcatcg agggcccgct ccccgcccgt cgtctggaca ctaataccat
    10321 caccgtcagc aaggaaaccc ttcccaagga aacggaaatc ctgatcctga aggtttcaag
    10381 ggaaggagac ctttagaatg gggaatgcgg attagaagcg taggggcgac ccggtgggtc
    10441 gcccctaagg tttgttacca tgttgcgcac catttgactt tttagagaat aaaatcaatc
    10501 ctacccatcc tcttaccagc actgctaacg agagaaaaaa ggtttcgtga acaaaaggga
    10561 agaaatctga actttctttt gagctccctt tcgaattccc ttaaaacttg acaaatccgc
    10621 ctaattcccc taatttttaa ggaggcggga tgatccttat tgatagcc
//
LOCUS       DASPRR010000446         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_759072, whole genome shotgun sequence.
ACCESSION   DASPRR010000446 DASPRR010000000
VERSION     DASPRR010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759072"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /gene="feoB"
                     /locus_tag="VEL68_16410"
     CDS             <1..2025
                     /gene="feoB"
                     /locus_tag="VEL68_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HXZ37606.1"
                     /translation="QGPLGGPIAIEVHGFQLALGRGVASQVFVDATGGMTIALAGQPN
                     SGKSTLFNHIAVYKAMTANFPGTSVTFTETRTRIDGLTCNCIDLPGTYSLTSQEPAEL
                     EARKHLLFGEVDLIINVVDASLLSRSLELTLELLELEVPMILCLNMMDEAEKKGVQIE
                     IPKLEQVLGIPVVPVIATTGKGVRELFAQVSRVWEEKRKGRVQKFSKDVEEEIQPLVA
                     LLEPEKRVNERVPARFLAIKLLEKDESFVEEVKQAGDFYHQVVPFQEKLQESHGVPSD
                     EVISSERHALAMNIFEKVARIIRSPKRDLRTALDRWVMDRFFGYGILALVFFAFFNFI
                     FGIGGLIEGPLIQFFDRLNNNLESLFPTGSLSLFLAHGILHGISGGIAIVLPYLVPFL
                     IGLSLLEDVGYLPRVAFLMDTFMHRIGLHGKSVIPFILGYGCSVPAVMSTQILEIPQH
                     RFIVSVLTTMIPCAARITIIFGLVAFFIGANAALAIFALNLLIVAIFGKVLTHFSPES
                     NLGFILEMPSYQFPALQTTLKKSWCRIREFIVIAWPLLIIGSAVLSLIEYWKLDHLIN
                     YLLLPLTRILDLPFAVGTTLLFGLLRKELSLIMLTQALGTTQVNTVMTHTQILVFTIF
                     VTFYIPCLATVGVLWKEIGKRGALLAMGFTLGIATVLGLLTRLFGEIFLN"
     gene            complement(2112..3524)
                     /locus_tag="VEL68_16415"
     CDS             complement(2112..3524)
                     /locus_tag="VEL68_16415"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXZ37607.1"
                     /translation="MNLLNDENLTIAKMAPLIRKKKISPVEVTETFLGRIQQLQPKLN
                     AFITVTADLARKKARQAERDLLKGKYHGPLHGIPISLKDLFYTAGIRTTGGSKILRSF
                     VPRENAAVVDRLYAAGAILLGKTNLHEFAYGVTNNNPHYGPVRNPWDLKRVPGGSSGG
                     SAAAVSAGLGMASLGTDTGGSIRIPSAACGVVGLKPTRGLVSLHGVIPLAFSLDHVGP
                     ICRCVEDTALIMEAIAAPGGPNTGNWPQVGRLFTRSLRGGVKKTRIGIPKQYFFDRLQ
                     KEVREFVLTACATLSQLGAELREVEFKGMMEAAPLVGVITVGEALSYHWKWLQSRAKD
                     YGPDVRERMEANRNQRTIEYLQAQEKRRTYTEAFLKVFDSVDVVAAPTLPVLPPFIGQ
                     KEIFWGRTREDVRAALLRMTRPGNLTGLPAVSLPCGFTADSLPVGLQLFGRHYDESTL
                     LRVAYAYEQATPWHKMFPPL"
     gene            3752..>4078
                     /locus_tag="VEL68_16420"
     CDS             3752..>4078
                     /locus_tag="VEL68_16420"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXZ37608.1"
                     /translation="MERKKVTVPDILRMKKEGRKITKINLHDYPTAVLADRAGVDMVM
                     IGDSIGMVVLGYTNTLPVTMEEMIHHAKAVVRGAGSCLVVCDMPFMSYQTSVDEAIKN
                     AGRLMKE"
BASE COUNT          845 a         1166 c         1164 g          903 t
ORIGIN      
        1 cagggtcctc tgggaggacc cattgccatt gaggttcatg gtttccaatt ggccttgggg
       61 agaggggtcg cttctcaggt ctttgtggac gcaaccgggg gaatgaccat cgccctggcc
      121 ggccagccca acagcgggaa aagcactctt ttcaaccaca tcgccgtgta caaggcgatg
      181 accgccaact ttccgggaac cagcgtcacc ttcaccgaga cccgtacccg gatcgatggt
      241 ttgacctgca attgcatcga ccttcccggt acttactccc ttacttccca agaacctgcc
      301 gaactggaag cccgcaagca cctcctcttt ggggaggttg atttaatcat caatgtggtc
      361 gacgcttcgc tgctgagccg cagtctggag ctgacccttg agctcctgga actggaagtc
      421 cccatgattc tttgcctcaa catgatggac gaggcggaga agaaaggggt ccagattgaa
      481 attccgaagc tggagcaggt cctcggcatc cccgtggtcc cggtcatcgc caccaccggg
      541 aagggggtaa gagagctctt tgcccaggtc agccgggtat gggaagagaa gagaaagggg
      601 agagtccaga aattcagcaa ggacgtggaa gaagagatcc agccgctggt tgcccttctg
      661 gagccggaaa agcgggtgaa cgagagagtc cccgcccgtt ttctggcgat caagctgctg
      721 gagaaggacg agtccttcgt ggaggaggtc aagcaggcag gggactttta ccaccaggtg
      781 gttcccttcc aggaaaaact ccaggagagc cacggggtgc cctccgatga agtcatctcc
      841 tcggaacgcc acgctctggc catgaacatc tttgaaaagg tcgcgcggat catccgttcc
      901 cccaagaggg accttcgcac ggcccttgat cgttgggtca tggataggtt cttcggctac
      961 gggatcctag ccttggtttt cttcgctttt tttaatttca tcttcggaat cggggggctc
     1021 atcgaaggcc cgctcattca attctttgat cgactcaata ataatctgga gagtcttttt
     1081 cctacgggca gcctgtccct ctttctggcc cacgggatcc tgcacggaat ctcggggggg
     1141 atagccatcg tgcttcccta cctggtcccc tttctgatcg ggctttccct cctggaagat
     1201 gtgggctact tgcccagggt ggctttcctg atggatactt tcatgcaccg catcggcctc
     1261 cacgggaaat cggtcatccc cttcattctg ggttacggct gcagcgtccc ggcagtcatg
     1321 tccacccaga tcctggagat tccccagcat cgcttcatcg tatccgtcct taccactatg
     1381 atcccctgcg cggcgcggat tactatcatc ttcggactcg tcgccttttt tatcggggcc
     1441 aacgcggccc tggctatctt tgccctcaac ctcctgatcg tggccatttt tggcaaggtc
     1501 ctaacccact tttctccgga gagcaacctg gggttcatcc tggaaatgcc ctcttatcaa
     1561 tttcccgccc tccaaaccac gctgaaaaag tcgtggtgtc gcattcggga attcatcgtc
     1621 attgcctggc ctctactgat catcggaagc gcggtacttt ctctgatcga gtactggaag
     1681 ctggaccatt tgatcaatta ccttctgctc cccctgaccc ggattctcga ccttcccttc
     1741 gccgtgggaa ccactctcct cttcggcctc ctgcggaaag aactctccct gatcatgctc
     1801 acccaggccc tgggaacgac ccaggtcaac acggtgatga cccacactca gatcctggtc
     1861 tttaccatct ttgtgacctt ttacatcccc tgcttggcca cagtcggagt tctttggaag
     1921 gaaatcggga aaaggggagc ccttctggcc atgggattca ccctggggat cgccaccgtc
     1981 ttgggccttc tgacccgtct gttcggggaa atcttcctta actgaattgc ggattgcaga
     2041 ttgcggattg cggattggaa aagaggaagg gaaaaaattc cgaaatccga aatccgcgat
     2101 ccgaagttgt ctcatagggg cgggaacatc ttgtgccaag gggtggcctg ttcgtaagcg
     2161 taggccacgc gcaatagggt cgactcatcg tagtggcggc cgaagagctg caatcccaca
     2221 ggaaggctgt cggcggtgaa tccgcaagga agagagacag ccggaagtcc ggtcaagttt
     2281 cctgggcggg tcatgcgcaa aagggcggcc cggacatcct cccgggtccg cccccaaaaa
     2341 atttccttct gcccgatgaa aggcggaagg accgggaggg tgggggcggc taccacgtcg
     2401 acggagtcga aaactttcag gaaagcctcg gtgtaagtcc gccttttttc ctgcgcctgg
     2461 aggtattcga ttgtccgctg gtttcggttg gcctccatcc gttcccggac gtccggtcca
     2521 taatccttgg ccctcgactg gagccacttc cagtggtagg acagggcctc ccccacggtg
     2581 atgaccccca ccagtggggc cgcttccatc atccccttga attccacttc ccgcagctcc
     2641 gctcccagct ggctcagcgt tgcgcaagcg gtcaggacga attcgcggac ctctttctgc
     2701 aaacgatcga agaagtattg cttgggaatt ccgattcggg ttttcttgac ccctccgcgc
     2761 aaagaacggg tgaacagacg gccgacctga ggccagttgc cggtgtttgg cccacctggc
     2821 gcggcaatgg cttccattat aagagctgta tcttctacgc agcggcaaat cgggcctacg
     2881 tggtcgaggc tgaaggccag ggggatgact ccatggagcg agaccaggcc ccgggtgggc
     2941 ttcagcccta caaccccgca agctgcggaa gggatccgga tcgatcctcc cgtgtcggtt
     3001 cccagagaag ccattcccag acccgcgctc acggccgccg cgcttccgcc gctggagcct
     3061 ccaggtaccc tcttcaaatc ccagggattg cggaccgggc cataatgggg gttgttgttg
     3121 gtcaccccat aagcaaattc gtgcaggttg gtctttccta gaaggatcgc cccggccgca
     3181 tagagtcggt ccaccacggc ggcgttttcc cgagggacga aactgcggag gattttagat
     3241 ccccccgtcg tccggattcc ggcggtgtag aacaggtcct tcagggatat ggggatcccg
     3301 tggaggggac cgtggtattt ccccttcagg agatcccttt ccgcctgccg ggcctttttc
     3361 cgcgccaagt cggcggtgac ggttatgaag gcattcagct tcggctgaag ctgctggatt
     3421 cgcccgagga aggtctcggt cacctcgacg ggggagatct tcttcttgcg gatcaggggg
     3481 gccatcttgg caatagtcaa gttttcgtca ttcaagagat tcatgcggcc tccgagcgag
     3541 tcaaggcaat aataaaatca tcgatggagg ggcggttcac gaaccacccc gcttttctct
     3601 ctgatgaagg cgagttgatt atagaaagcg agaaacggcc tgtcaaacga aaaggctggt
     3661 tgcaaatcgc caaagcttgt gctaccagat aggcaggatc gatgaagcgg aaaaaaatga
     3721 cttcgaaagc gaaagcaggg gaggttttgc catggaaaga aagaaagtta ccgtgccgga
     3781 tattctgcgg atgaaaaaag agggccggaa gatcaccaag atcaaccttc atgactatcc
     3841 cacggcggtt ctggccgacc gggcgggagt cgatatggtc atgatcgggg attccatcgg
     3901 catggtggtc ctggggtaca ccaacaccct cccggtaacc atggaagaga tgatccacca
     3961 tgccaaagcc gtggtccgcg gggctgggag ttgcctggtg gtctgcgaca tgccgttcat
     4021 gtcctaccag accagcgtgg atgaggcgat aaagaacgca ggccggctca tgaaggag
//
LOCUS       DASPRR010000447        11155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2042521, whole genome shotgun sequence.
ACCESSION   DASPRR010000447 DASPRR010000000
VERSION     DASPRR010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11155)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11155
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042521"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VEL68_16425"
     CDS             <1..286
                     /locus_tag="VEL68_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37609.1"
                     /translation="TYRNGAWFLDLNGNGVWDGCGTDAYIASFGLPTDIPVTGDWPGS
                     GTTKIGTYRNGAWFLDLNGNGVWDGCGVDLCIPSFGGFPGDLPITGKPGR"
     gene            321..827
                     /locus_tag="VEL68_16430"
     CDS             321..827
                     /locus_tag="VEL68_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXZ37610.1"
                     /translation="MAGNERLIKIFEHALQQEFTGRDFFKNSLQRMGWGSAVTAFQRL
                     IQEEEKHIEMIEKILSGLRKGSSVDLPSPKGPEEAVSPFEERAKKEFLAQCLEGSMVP
                     EVTIFNTAWLIEKDLSEFYEKMARQTEGKAREALMYLSRWEQAHEKLFREFRDQFAEM
                     YSKIPWGG"
     gene            complement(1278..3218)
                     /locus_tag="VEL68_16435"
     CDS             complement(1278..3218)
                     /locus_tag="VEL68_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ37611.1"
                     /translation="MAPKSVHFPKIFEPAFLGPLEVRNRLIMPPMGTRLANENGGVSP
                     WQIDYYAERAKGGVGTIIVEITGVDSPHGVASPKTLTIHEDFYIGGHNELVEAVHAHG
                     ARIFLQLAHVGRNRRLAMGVQPVAPSPIPNRFFGVTPRELSCGEIEDLVKKFIAAALR
                     AQTAGYDGIELHGAHGYLIGEFISPLSNRRKDRYGGSLEKRMAFPVEIVQGIRRATGP
                     RYPILFRFSADEFEEGGTTAQDSKKAARILEEAGADALHVSAGTYDSMARTIEPMSYP
                     EGWKIYLAETIKRAVKIPVIGVGVIRSPEFAERTLQEGRADFIALGRALLADPHWPQK
                     AREGRVKEIIPCISCNECIGGRTFRNLHIRCAVNPATGREGWLQRLPSEKKKKVVVIG
                     GGPAGLMAALTAKRRGHRVTLYEKSDHLGGQLWLAAAPPGREKISWFSDYLRNQIRQE
                     KIKVLLGITATPEKIAREKPDDVILATGSVPLFPDLPGVESPQVCTAWDILGGKKIVR
                     DKVVLILGGGMVGCGTALYLAARGNKVIVVEMLEGAAQDTDFITQADILFRMKEARIK
                     ILAGRKAEKISDGEFFLRNSAGKGERIKGDIVVLALGSVPAGDLKKSLQRKVKEVHTI
                     GDAHQPGKIIDAVYEGFRLNGG"
     gene            3217..3435
                     /locus_tag="VEL68_16440"
     CDS             3217..3435
                     /locus_tag="VEL68_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37612.1"
                     /translation="MEEILLAGLGYDIIKAFLPIEGEKIGTKKAIASRRALRDRREEE
                     IQDSSPGFRIPDRGIVHQHDICTRAGPN"
     gene            3787..4812
                     /locus_tag="VEL68_16445"
     CDS             3787..4812
                     /locus_tag="VEL68_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005241914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HXZ37613.1"
                     /translation="MRQWVADKAVTQEEGEKLLESYEIAGFDWKRLAKYSFWISLVCI
                     VIAVGSILADRALMALLAKIFHAPDIIKCLFLAGIAAALFSAGLRRRRKKPEKVFSNE
                     AIFSLGVFATAGALAFFGKAIDTGRGHFSILVLLAAIIYAILGLWFPSKLIWVFALLS
                     LGSWFGAETGYVSGWGAYYLGMNYPLRFVIFGAVLLAAGSLFTVKLGRQDFGKPTRVM
                     GLLYLFVALWILSIFGNYGEIDSWSRAKQIELFHWSLLFGVVSLLVILHGIKFDDPMM
                     RGFGLTFLFINLYTRYFEYFWDAIHKAIFFALLAISFWFLGSRAERIWNLSILKRRTS
                     SQHSDKG"
     gene            complement(5140..6006)
                     /locus_tag="VEL68_16450"
     CDS             complement(5140..6006)
                     /locus_tag="VEL68_16450"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXZ37614.1"
                     /translation="MIEKRPFGRTGHMSTVAIFGAAALMGVGEKEADRALEILLEFGV
                     NHIDTAPRYGDSEVLIGPWMDRYRKDFFLATKTKKRTYREAREEIRRSLERLRVGQVD
                     LIQLHAIGHPDEWDAALGPGGALEAAVEAREQGLTRFIGVTGHGWMIAAMHKRSLLRF
                     DFDSVLMPYNYLMHQNEIYRKDCESVVSMCRERNVAVQTINSIARGPWGITPQNRNTW
                     YQPLEEQADIDKAVHWVLGNPDVFLNTAGDVGLLPKVLDAASRFQRRPAPGEMDELVK
                     KNKMSSLFGLGP"
     gene            complement(6163..7386)
                     /locus_tag="VEL68_16455"
     CDS             complement(6163..7386)
                     /locus_tag="VEL68_16455"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HXZ37615.1"
                     /translation="MTEVGVSDMAIYVPPFYLAHEDLGRARGVPPEKYHLGLGNYSIS
                     ILPNWEDGVTMAANAAAQLLEKTGTVPDEIGQLVVSTESGVDHSKPVASFVQGLLGIG
                     ERCRIYEIKHACYGGTAGLVNSIDWISRNCHAPKKALVIMSDIARYAFGSPGEPTQGA
                     GAVALLVERNPRLFSVDTRMNGIFSKDVFDFWRPTGHRVPIVDGKYSIDCYLEALQGA
                     YSHFRSNLGVERGKLLEKIDYLIYHMPFSNMARKAHRHLIDLEYDGLEPSVKERLFEE
                     TFARKVEPSLLGSRQVGNIYTGSVYMGLISLLETEKEKAEGKRVGIFSYGSGCGAEFF
                     LCQMKSGIEGIIRGLRFQELLERRKKITFEKYVQTYSKSWEETFYHPGEVEVFKDDFT
                     RFVFTGFKDHKRQYL"
     gene            7946..9226
                     /locus_tag="VEL68_16460"
     CDS             7946..9226
                     /locus_tag="VEL68_16460"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HXZ37616.1"
                     /translation="MHTIRDAKISGQQSITMAGKGQYDVAPGVKRRTFLSACLGIAIA
                     FVLGLDMSSVQAQVGGASAAAIASGKSGPVFAYVGCRTTKERNARGEGINVYRVDPSS
                     GSWTHVQLVGNLVNPSFLAFDRRQNFLYSVHGDFSEVSSFKVNRQTGELTLLNRQSTQ
                     GKNPVHLVVDPTNRFLVISNYATGTLALLPIQEDGTLGPLKDFAKLPGNPGPHKVEQT
                     ISHPHHNPLDPGERFIIVPDKGLDKIFTFRIDVENGKLIAGPSVETRETSGPRHIVFH
                     PGKAYAYVINELDSTVTTYRYNAERGELAPMQILTALPATFTGNNRSSEIAITTSGRF
                     VYASNRGHDSIAIFAVDQTTGMLSSVGWESTQGKNPRFFSLDPTGAFMYAANENSDTI
                     VTYWIDPVTGKLSPTGQIIKTGSPVCIIFSSGGP"
     gene            complement(9598..10557)
                     /locus_tag="VEL68_16465"
     CDS             complement(9598..10557)
                     /locus_tag="VEL68_16465"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXZ37617.1"
                     /translation="MFEGESDPRGVQPKVLSRLQSLGFEVVLVKKGQSILSQGSGFII
                     SRDGHILTCAHLFKDKKEATVWVGGKRYEADVMNKDARYDLAIVKVRSPSDVVHKPLA
                     LSRTDKFSMGQDVFTMGFPLSSILGNTPRLNKGLINATVGIRDDPDQLQVSVQIQPGN
                     SGSPLFNDQREVIGIIFSTLSSVDVLRQTRGVLPQNVNFALKAGPIRKFLEQSGIQQN
                     PSAEIVPPSSFDEAKESIVRIYSGIEPQDKPPELICSIFYQYIEYWNRFRAFRLQFYD
                     RETGKLLLEVGQYKDTELSTEDEVLNRALKEVGKQFFPPAKSN"
BASE COUNT         2536 a         3034 c         2879 g         2706 t
ORIGIN      
        1 gacctacagg aacggtgcgt ggttcctcga cttgaacggc aatggagtct gggatggctg
       61 cgggaccgac gcctacattg cttcctttgg tctgccgacc gacattccgg tgaccggcga
      121 ctggcccggt tccggcacca ctaagatcgg gacctacagg aacggtgcgt ggttcctcga
      181 cttgaacggc aatggagtct gggatggctg tggggttgac ttatgtattc cttcttttgg
      241 aggttttcca ggtgaccttc cgatcactgg aaagccagga aggtgagcgc ggaatcctgg
      301 caaggaaggg agatagaacg atggccggca atgagaggct gatcaagatt ttcgaacacg
      361 cccttcaaca ggagtttacg gggagagact tcttcaagaa ctcccttcaa aggatgggat
      421 gggggtccgc ggtgaccgcc tttcagcgtc tcatccaaga agaggagaaa cacattgaaa
      481 tgatcgagaa gatcctttcc ggcctcagga aaggatcctc cgtggacctc ccctccccaa
      541 aaggccccga ggaggcagtc agtccttttg aggagagggc caagaaagag ttcctggccc
      601 aatgcctgga gggctccatg gtccccgagg ttacgatctt caacaccgcc tggctcatcg
      661 agaaagacct gagcgaattc tacgaaaaga tggcccgcca gacggaaggg aaagccaggg
      721 aagctctgat gtatctttca cgctgggagc aagcgcacga aaagctgttc cgcgaattcc
      781 gcgatcaatt cgccgagatg tactccaaga ttccatgggg agggtaatga ccctgcccct
      841 aatccccttg ggctttccaa gaggcttttt ccccgcatcg aaagcaccgg tttaagataa
      901 gagggtcttc tcccaatcga gtctgttgaa agagaaccga aaatgttcta tcgctcaggc
      961 ccagggggtg tgtcccatcc atggcaggac agcattggcg ggaaaaacgc cggattccgg
     1021 agcggttgcc cgggtttcgc aaggcacctg tctccgaccc ccttggggaa aaccgaatcg
     1081 tggacctaat cggtccgacc taagtatgtt ccgacgcgaa cccatgaaag aaccaccgcc
     1141 tcatgatgtc ctgccatgga tgggatgcac cccgtgggcc cgggccaatc ccctcccggc
     1201 aggactatcc cggcccgaat gatgaggtct cccgaacttt ctcccggtcc cgtatgggaa
     1261 attacccccg cgccctttca cccgccattt aaccgaaaac cttcatagac tgcatcgata
     1321 atctttcccg gctgatgggc atccccgatt gtatggactt ccttgacttt cctctgaagg
     1381 ctctttttca ggtcccccgc gggcactgac cccaaagcca gaacgacgat atcccccttg
     1441 atcctttctc cctttcccgc tgaattcctc aggaaaaatt ccccgtcgct gattttctcc
     1501 gcctttctgc ctgccagaat ttttatccgg gcttccttca ttctgaacag aatatcggcc
     1561 tgggtgatga aatccgtatc ctgcgcggct ccttccagca tctcaacgac gataaccttg
     1621 ttgccccggg cagccaagta gagggcggtc ccgcacccca ccattccgcc ccccaggatt
     1681 aaaacgacct tatcccgtac tatctttttc cctccgagga tgtcccaggc cgtgcatacc
     1741 tgcgggcttt cgactcccgg caggtccgga aacaacggaa cggaacccgt ggccagaatt
     1801 acgtcgtcgg gcttctcacg cgcgatcttc tcaggagttg ctgtgattcc caggagaacc
     1861 ttaattttct cctgccggat ttgattccga agataatcgc tgaaccagga aattttctct
     1921 ctgcccggcg gcgcggccgc cagccaaagc tggcctccca gatgatcgct cttttcatac
     1981 agcgtaaccc ggtgcccccg tctcttggcc gtcagagcgg ccatgagacc tgccggcccg
     2041 ccgccgatta ccaccacctt ttttttcttt tccgaaggaa gcctttgcag ccacccttcc
     2101 cgccccgtcg cgggattcac ggcgcagcga atatgcagat tgcggaaagt gcggcctccg
     2161 atacactcgt tgcacgagat gcagggaatg atctctttca cccttccctc cctggccttc
     2221 tgcggccaat ggggatcggc cagcagagcc cttcccaggg caatgaagtc ggccctcccc
     2281 tcctgcaggg ttcgttcggc gaactcgggg ctgcggatca ccccgacgcc gatcaccggg
     2341 attttaaccg ccctcttgat ggtctccgcc aggtagattt tccacccttc cggataggac
     2401 atgggttcaa tggtcctggc catggagtca taggttcccg cgctaacgtg cagggcgtcg
     2461 gcaccggctt cctccaggat cctggccgct ttcttcgaat cctgggcggt tgttcctccc
     2521 tcttcgaatt catcggcgct gaagcggaag aggatgggat agcggggacc ggtcgcccgg
     2581 cgaatccctt ggacgatttc caccggaaag gccatccgct tttccaggct cccaccgtag
     2641 cggtctttcc ggcggttgct gaggggggaa atgaattctc cgatgaggta cccgtgggct
     2701 ccatgaagct cgattccgtc atagccggcg gtctgagcgc gcagggccgc agcgataaac
     2761 ttcttgacca aatcttcgat ttctccgcag ctcagctccc ggggggtgac cccgaaaaat
     2821 cgattgggaa tcggggaggg agccaccggc tggactccca tggcgagcct ccggttccgg
     2881 cccacatggg cgagttggag gaagatcctg gccccatggg catgaacggc ctccaccagc
     2941 tcgttatgcc cgccgatgta gaagtcctca tggatggtca gggttttcgg gctggccacc
     3001 ccgtgaggag agtcaacccc ggtgatttcc acgatgatcg taccaacccc ccctttggcc
     3061 ctctccgcgt agtaatcgat ctgccacgga gagacacccc cgttttcatt ggccaggcgg
     3121 gtccccatgg gcggcatgat gaggcggttg cggacttcca gcggccccaa aaaagcgggc
     3181 tcaaaaatct taggaaagtg aacggatttc ggcgccatgg aagaaatcct ccttgcaggc
     3241 ttaggttacg acattataaa agcttttctc ccaatagagg gggaaaaaat aggaacaaaa
     3301 aaagcaattg cctctcgcag agccctcagg gaccgcagag aagaagaaat acaggattct
     3361 agcccgggtt ttaggattcc cgaccgcggc attgttcacc agcatgatat atgcacccgg
     3421 gcaggaccaa attaactact tgatattgct tctaaagatt cgaaaaagga tgccgaaaaa
     3481 gggcataggg gtgcaatttt ccctttaact tgttttccag ctaactgagg tccaatgaat
     3541 cgccttctgg tccaactctg aattgagaaa tggacgcacc aaattgacct atggttttga
     3601 tgatgaagag gtaaccaaca gggggggttg atgaagacta ttttcatatt ggtcattttc
     3661 tgtttttgcc tcttttcctg gtagctatcg catgttcccg ctgcgcgccc gcatgttaaa
     3721 tgccagaatg gatagaggat cgaattgaaa ctgacaagaa gaaaggcaaa attggtggag
     3781 gcggtcattc ggcaatgggt agccgacaag gctgtaactc aagaagaagg cgaaaagctt
     3841 cttgagagtt atgaaattgc aggttttgat tggaaacgcc ttgccaaata ttccttttgg
     3901 atttcattgg tctgcattgt cattgctgtc ggatcgatac tcgccgacag ggcattgatg
     3961 gcattacttg ccaagatatt ccatgcccct gacatcatta agtgcttgtt tttggccggc
     4021 attgctgcag ccctgtttag tgcaggtcta aggagaagaa gaaagaaacc cgagaaagtc
     4081 tttagcaacg aggccatatt ctcgctcggc gtattcgcca ccgctggggc gcttgcattc
     4141 ttcggcaagg caattgatac gggaagaggc catttctcga ttctcgtcct tcttgctgcg
     4201 atcatatacg caatactggg gctttggttt ccgtcgaaac tcatctgggt ctttgctctt
     4261 ctttcgttgg ggagctggtt tggtgctgaa accggatatg tgtcggggtg gggagcttac
     4321 tatctcggga tgaattatcc cctcaggttc gttatcttcg gtgcagtttt gttggccgca
     4381 ggcagcttgt ttacggttaa acttggacgg caggattttg ggaaacctac tcgggttatg
     4441 ggcttgcttt atctttttgt agcactctgg atcttatcga tttttggaaa ttatggagag
     4501 attgactcgt ggtccagggc caaacagatc gaactgtttc attggtcctt gctctttggc
     4561 gtggtttctc ttctggtaat tctgcatgga atcaagttcg atgaccccat gatgagaggt
     4621 tttgggttaa cctttctgtt tatcaatctg tatacgcgat actttgagta tttttgggat
     4681 gccattcata aggcaatatt cttcgcattg ctggccataa gcttttggtt tctgggttca
     4741 agagcagaga ggatatggaa tctctctatc cttaaacgtc gaacctcatc ccaacatagc
     4801 gataagggat gaagtatggt atttaacaag gcaatccagc cggcggagaa gagtagtcgg
     4861 agcgagggac cttatcggtt acgcagcgat ttgtggatac ggggtgtctc tgacgcatga
     4921 ggccaaggtc ttgaatatat tcgtgcagag cgttcttcgt acggcggatg gaggggagca
     4981 ggaatttcaa aatgggggat gggcgattgc aggttcccta caataacgca gttatcggag
     5041 ctacgtcccc cctccttccg cgaatgctct gtccttatgg cctgaaagcc ggaggggata
     5101 gcggcttgca ggaagaggga gttcaaaaac tctgcaaatt taggggccga gtccgaacag
     5161 ggaagacatt ttatttttct tcaccaattc atccatttca ccgggtgccg gacgcctttg
     5221 aaagcgggag gctgcgtcca gaactttggg taatagaccc acatcgccag ccgtgttgag
     5281 aaatacatcc ggattcccca gtacccaatg aaccgccttg tcaatgtccg cctgctcttc
     5341 cagcggctgg taccacgtgt tccgattctg cggggtaatg ccccatggac cgcgagcgat
     5401 ggaattgatg gtttgaacgg ctacgttcct ttcccggcac atgcttacca cagactcgca
     5461 atccttccgg tatatttcat tctggtgcat taaatagttg taaggcatca gaaccgagtc
     5521 aaagtcaaag cgcagcaggc tgcgcttgtg catggcggcg atcatccatc catgacccgt
     5581 taccccgatg aatcgtgtca ggccctgctc ccgcgcctcg accgcagctt ccagggctcc
     5641 tccgggtcca agtgccgcat cccactcatc cggatgacca atggcatgga gttgaatcag
     5701 atcgacctgg ccgactctta gacgttcgag agaccggcgg atttcttcgc gggcctcgcg
     5761 gtaggtgcgc ttcttggtct tggtggccag gaaaaagtcc ttgcggtagc gatccatcca
     5821 gggcccgatg agtacttccg aatctccata gcgcggggcg gtatcgatat ggttcacccc
     5881 gaattccagg agaatctcca gtgcccggtc cgcttccttt tctccgacac ccatcaaggc
     5941 cgcagccccg aagatcgcca ccgtactcat gtgcccggtc cgtccaaagg gtcttttttc
     6001 gatcaaatca cagtctcctc cagaattgat ctcataccaa cgaaaaaatc ccagttcgtt
     6061 cgtggaatcc acctccattt gggcaggttt ctttatgcgt ttacattgtg ccctgctcgc
     6121 cttgcctaaa gcatctatcc aggggcgaag agaaggaagg agttatagat actgccgctt
     6181 gtggtccttg aagccggtaa agacaaatcg ggtaaaatca tccttgaaaa cctctacttc
     6241 cccagggtga tagaaagtct cctcccagct cttggagtaa gtctgcacgt acttttcgaa
     6301 ggtgatcttt tttctccttt ccaaaagctc ctggaagcgc aagccccgaa tgataccttc
     6361 gattccgctt ttcatctggc aaaggaagaa ctcggctccg cacccgcttc catagctgaa
     6421 aataccgacc cgttttccct cggctttttc cttttccgtt tccagaaggc tgatcagccc
     6481 catatagaca gaacccgtat agatgtttcc cacctgacgg gaccccaaaa gacttggttc
     6541 gacttttcgg gcaaaggtct cttcgaaaag cctctctttg actgaaggtt ccaaaccgtc
     6601 atactcgaga tcgatcagat gacgatgcgc tttcctggcc atgttgctga aaggcatgtg
     6661 atagatcagg tagtcgatct tttcaagtag ctttcctctc tcgactccca ggttggaacg
     6721 gaagtgggag taagcccctt ggagggcttc caggtaacaa tcgatcgagt atttcccgtc
     6781 tacgatggga acccggtggc cggtcgggcg ccagaaatcg aagacatcct tggaaaaaat
     6841 tccgttcatc ctggtgtcta ccgaaaagag gcgtggattt ctctccacaa gcagagccac
     6901 ggccccggcc ccttgagtcg gctcccccgg agaaccaaaa gcgtaccggg caatatcact
     6961 cataatcacc agggccttct tgggagcatg acagttccgc gaaatccagt cgatggaatt
     7021 taccaggcca gcagtccctc cgtagcaggc atgtttgatt tcataaatcc ggcacctttc
     7081 cccaattccg agcaatccct gtacaaagct ggccaccggt ttggagtgat ccaccccgct
     7141 ctccgtgctc accacgagct ggccgatttc gtcggggacg gtccccgtct tttccaaaag
     7201 ctgggcggcg gcattggccg ccatggttac tccgtcctcc cagttgggaa gaatcgatat
     7261 actgtaattt ccaaggccaa ggtggtactt ctccggcggg actccccggg ctctgccgag
     7321 atcttcatgg gcgaggtaaa agggggggac atagatcgcc atatcgctga ctccaacctc
     7381 cgtcatcctg cttcctcctg tatctggttt ttctgggcaa cactgagggg atttaaaaaa
     7441 tataacccgt gattatccta ctagtcaagt aaagaatagg ccgaagggat aggggaaaga
     7501 tagaggcggg gggtcatagg tttggagggc tatatagata tttacaacat ttacggtaca
     7561 ctgattcgtg cggtgcattt gatgggggag ggtgcattaa tgacccctgg gcttgaagaa
     7621 acttggaggc ttagatcaca ggtatgagaa gagatacacc gccatgcttt taatgagatt
     7681 cgcatgtagg cttattgaaa tccaggacac aacgtcccgc accccaaagc acgttcaaca
     7741 agaatgttcc ctcattcccc aaaaattaat gtgagccaaa agcagacttg cccccttcct
     7801 gacgggctca ctcttatctt tttgcaggag gtttcttttt tcatggattt caggtaacat
     7861 acccagaagg ttttcttcct tcaccctctg gagtgggttc tcccttgttt ttaatatatg
     7921 acgttatgga aaaggaggtg ctgtgatgca caccatccgt gacgcgaaaa tttccggcca
     7981 acaatcaata accatggcag gcaaaggcca gtacgatgtt gccccggggg tcaaacgaag
     8041 aacgttcctg tcagcctgtt taggtatcgc gattgcgttt gtactgggtc tcgacatgtc
     8101 atcggttcaa gcccaagttg ggggggcaag cgcagcagcg attgcgagtg gcaaatccgg
     8161 tccggtgttt gcctacgtcg gctgccgtac gacgaaggag cgaaatgccc ggggggaggg
     8221 aatcaatgtc tatcgcgtcg atccgtcctc cggctcctgg acgcatgttc agcttgtcgg
     8281 gaatctcgtg aacccttcct ttctggcctt tgaccgccgg cagaacttcc tctattcggt
     8341 ccacggcgac ttcagcgagg tcagcagctt caaggtcaac agacagaccg gtgaactgac
     8401 cttactgaac cggcagtcaa cccagggaaa gaatccggtc cacctggtgg tggacccgac
     8461 gaaccggttc ctggttatat cgaactacgc gaccgggacg ttagccctct taccgatcca
     8521 ggaggacggc actctggggc cactcaaaga ttttgccaaa ctgccgggca atcccggacc
     8581 gcacaaggtt gagcagacaa tctctcatcc gcaccacaac cccctggacc cgggagagcg
     8641 gttcatcatc gttcccgaca aagggctcga caagattttc acctttcgta tcgacgttga
     8701 aaacggcaag ctgatcgccg gtccctctgt cgaaacccgt gagacttcgg gaccgcggca
     8761 tattgttttc catcccggca aggcgtacgc ttacgtcatc aatgagctgg attcgaccgt
     8821 gaccacctat cgctacaacg ccgaacgcgg ggaactcgcc cctatgcaga tcctgacggc
     8881 cctgccggcc acctttaccg ggaacaaccg ttcctccgag atagccatca ccacctcggg
     8941 gcgtttcgtg tatgcttcca atcgcggtca tgattcgatt gccatctttg ccgtagatca
     9001 gacaaccggc atgctctcct ccgtcggttg ggaatcgacc caaggcaaaa atccccgctt
     9061 cttttcgctc gatccgacgg gtgcatttat gtacgcggcc aacgagaaca gcgatacgat
     9121 cgtcacatat tggatcgacc cagtaaccgg taaactatcg ccgaccgggc agatcattaa
     9181 aaccggaagc cccgtatgca tcattttttc atcgggtggg ccgtagtgac tgtgctcgcc
     9241 gttgcaggtg ctttgataaa ctccctaagt cataagtcaa ttcagaaagg acgtttcctt
     9301 ctacacaatt aatcggggga taaatttcat gttgagtgtt ttaaacctcc actttaaaaa
     9361 cctcaactcc ttattccgcc tggatgccgc cgatcatgcg atatcccgcc ttggagcggg
     9421 gccgtattac ctaagttttt cgcggacact aaagttcccc cctgctttgg gcaaagtaac
     9481 cttatatttc cattaagaat ttgtggcaga atacctcccc cggtttctca atcggccata
     9541 ggatgctcat ggaaaggact tggaagcggc ctgggaggct gaggattttg gtgattatca
     9601 gttggatttg gcgggcggaa agaattgttt cccaacttct ttaagggcgc gattcagtac
     9661 ttcatcttcg gtgctcaatt cagtgtcctt atattgtccg acttcgagca ggagctttcc
     9721 tgtttctcga tcataaaatt gaaggcgaaa cgctcggaag cggttccagt attctatgta
     9781 ctgataaaaa atggaacaaa tcaattccgg gggcttatcc tggggttcaa ttccactgta
     9841 aattcttacg atggattctt tggcttcatc aaaagaagaa ggcggaacaa tttcagccga
     9901 tgggttctgc tgtattccag actgctcaag aaatttgcgg atgggtcctg ctttaagggc
     9961 gaaatttaca ttctgaggca agacccctcg ggtttgcctc aatacatcca cggagctgag
    10021 ggtggaaaaa atgatgccaa taacctccct ctggtcattg aaaagagggc tcccgctgtt
    10081 gcccggctgt atttgtacag aaacctgcaa ttggtccgga tcatctctta tgcctacggt
    10141 cgcattgatg agtcctttat tcaacctcgg ggtatttccc aagatgctac tcagagggaa
    10201 ccccatcgtg aaaacatcct gccccatcga aaacttgtcc gtccgggaca gggccagcgg
    10261 cttatgaaca acatccgaag gcgaccttac tttcacaatc gccaggtcat aacgggcatc
    10321 tttgttcatt acatccgcct cgtatctttt accgccaacc cacacagttg cctctttttt
    10381 atctttgaac aaatgagcgc aagtgagaat gtggccatcc cggctgatga tgaagccgct
    10441 tccctgcgat aggatagact ggcccttctt gaccagaacc acttcaaagc ccagagattg
    10501 aagtcgagat aggactttgg gctgcacccc tcggggatca gattcacctt caaacaaata
    10561 cacctttttg tagttgggaa gaaattcgac gtgtttgact gtggacaggg gcgcaggggt
    10621 agcgcacccc aaaagaggga tccacgggag cactgcccaa aaagccgccc tcctcatccg
    10681 gagaagtatt tctttcatgg gattccccga cccaggagca aataccctca gcctactacc
    10741 accctttccg tagcctgctt aaagataacc atacggggtg gcagggatgt tcgtgcagag
    10801 cgtgtactcg ccatgccaat agtgcaaaga atgttcacca tgagcgtccc ctccctcctt
    10861 ggttcttcgg cattagcgtg tgcggaaaag gggacgtagg ttctgaagca acttaaacat
    10921 atttgggcta ctcccggggc tcaatcgaaa cagccacccg atttacagcg gaaatcgttg
    10981 ccgggacgat cggattgatc gcgatgcagg cgaaaatgtc gcgatcatgg ccgcagctta
    11041 cctcatcacc atccccaacc actgctgttc aagctcctgg acctcgcctt gtaggaacat
    11101 ttgctccagc cagaggttga gccaattcag gaaaatttgg tcgccctggc ggatc
//
LOCUS       DASPRR010000448         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1399372, whole genome shotgun sequence.
ACCESSION   DASPRR010000448 DASPRR010000000
VERSION     DASPRR010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399372"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VEL68_16470"
     CDS             <1..984
                     /locus_tag="VEL68_16470"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37618.1"
                     /translation="SLTGMEYTRRVNLIKDVNIPLLAITITKGGKMMDIFDEIVMEWS
                     CHDQTKANQAVAAGKGPLRIRVSHLPEEVIREIESILFSTERPVQERFFKGRGVDFRK
                     GDIELWPTENQLCGGHGMSGLVVNEKAETTVPGLYAAGDVACVSKGHLTGAFVFGEIA
                     AEQAVRFITSQPLPKIDPGQIAGMRSKIEKRGSRKGNGVDVRDLEFKVRRLINDYVIS
                     PKNAYKLSRWLEWAERFEREIEEDVSVSSGHELSKVMEVENIIQCATFSARAALERKE
                     SRWGNAHQRTDFPQRDDQNWLCHVDVRCGRDGEVLVSRRPLVRELPAGGAR"
     gene            981..>2565
                     /locus_tag="VEL68_16475"
     CDS             981..>2565
                     /locus_tag="VEL68_16475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ37619.1"
                     /translation="MRSDVNIFIHRDKCYVCGICIERCILDNLRMYLAPCRAACPIHM
                     NCQGYVRLIAQGKEEEAAKEARKDLPFVGIIGRVCHRPCEDQCERKKVDQEAVHIRAL
                     KRYLSDSRPDLIHEPAPMPKGSGRKVAVLGSGPAGLMAAYELGAKGHAVSVFDAAPEP
                     GGMLRWGIPAFRLPTAEINNAVSMLEKMKVRFQTGRSLGKELDLEKMEREWDALLLAY
                     GAGSAVALNIPGEDLPGVYQGLDLLRKVREGQTPDPGKSAIVIGGGNTAVDAALTCRR
                     LGAEEVQIICLEERSQMPAFAAELEEAVEEGIKIQNGWGPRKILQHGQDSLRLELSRC
                     LRIFDEEGRFSPQLDDFRGLSPSAESVIVAIGQQTDPSLLPPEIRPSPRSNQLVDPLT
                     LQSRREKIFAAGDLISGPRSVIESMAQGREAAVSIDRFLQGETLRWGRAYWDGTCITD
                     FPVDRSGAIVRPRASLPRLPVQSRNALNEIEKALGTKEAREEAERCLNCGRPGEVNQT
                     CWYCLPCEIECPVDALEVRL"
BASE COUNT          541 a          730 c          840 g          454 t
ORIGIN      
        1 agtctcaccg gcatggaata cacgcgccgg gtgaatctca tcaaagatgt caatattccc
       61 ctcctggcca tcaccatcac caagggcggg aagatgatgg acatcttcga cgagatcgtc
      121 atggagtgga gttgtcacga tcagaccaag gccaaccagg cggtggcggc cggtaagggg
      181 ccgctgcgga tccgggtgag ccacctcccg gaagaggtca tccgggagat cgaatccatt
      241 cttttcagca cggaacggcc cgtgcaggag cgcttcttca agggacgggg ggttgatttc
      301 cgcaaagggg acatcgaact ctggcccacg gagaaccagc tctgcggggg ccacgggatg
      361 tccggtctgg tggtcaacga aaaggccgag accacggttc ctggccttta tgccgcggga
      421 gacgtagcct gcgtttccaa agggcatctc accggagcct ttgttttcgg ggagatcgcc
      481 gccgagcagg cggtccgctt catcacctcc cagccgctcc ccaaaatcga cccgggtcag
      541 atcgccggca tgcggagcaa gatcgaaaag cggggaagcc ggaaggggaa cggggtggac
      601 gtgcgcgacc tggaattcaa ggtgcgacgg ctgatcaacg actacgtcat ctctcccaag
      661 aacgcttaca aactcagccg ctggctggag tgggcggagc ggtttgagag ggaaatcgaa
      721 gaggatgttt ccgtcagcag cggccacgag ttgtccaagg tcatggaagt ggaaaatatc
      781 atccagtgcg ccactttctc ggcccgggcc gccctggaga ggaaggagag ccggtggggg
      841 aacgcgcacc agcgcaccga tttcccccag agggacgatc agaactggct ctgccacgtg
      901 gatgtgcgct gcgggaggga tggggaggtt ctggtatccc gcaggccctt ggtccgcgag
      961 ctccctgccg gaggcgcgag gtgaggtccg acgtaaacat cttcatccat cgcgataaat
     1021 gctatgtgtg cggcatctgc attgaacgct gcatcctgga caacctgcgc atgtacctgg
     1081 ctccctgccg ggcggcctgc cccattcaca tgaactgcca gggctatgtc cgcttgattg
     1141 cccaggggaa ggaagaggaa gcggccaagg aagcgcggaa agacctcccc tttgtcggga
     1201 tcatcggaag ggtctgccat cgaccctgcg aagaccaatg cgaaagaaaa aaggtggacc
     1261 aggaggccgt ccacatccgc gccttgaagc ggtatctgtc cgactcgcgg ccggacctga
     1321 ttcatgaacc agccccgatg ccaaagggga gcggaaggaa ggttgccgtc ttggggtcgg
     1381 ggcctgcggg cctgatggcg gcctatgagc tgggggcgaa gggacatgca gtcagcgtct
     1441 ttgatgccgc ccccgaaccg ggagggatgc tccgctgggg aatccctgcc ttccgcctac
     1501 ccacggcgga aatcaataac gcagtttcga tgctggaaaa gatgaaggtt cgcttccaga
     1561 ccggccgaag cctgggcaaa gaactggacc tggaaaagat ggagcgtgaa tgggatgccc
     1621 ttctgctggc gtatggcgcg ggctcggccg tggctctgaa cattccgggg gaagacctcc
     1681 cgggagttta ccaggggctg gacctcctcc ggaaggtgcg ggagggccaa accccggacc
     1741 cgggaaagag cgcgatcgta atagggggag gcaacaccgc ggtcgatgcg gccctcacct
     1801 gccgaaggct gggagccgag gaagtgcaga tcatctgtct ggaagaaaga tcccagatgc
     1861 ccgcttttgc cgcggagctg gaagaagctg tggaggaggg catcaagatt cagaacggct
     1921 gggggccgcg aaaaatcctg cagcatggac aggacagcct gcggctggag ttatcccgct
     1981 gccttcggat tttcgacgag gagggccggt tctctcccca attggatgat ttccgcggac
     2041 tgtctccttc ggctgaatcg gtcatcgtgg cgatcggaca gcagacggac ccttcattgc
     2101 tgcccccgga gattcggcct tcccctcgat caaaccagct ggttgaccca ttgaccttgc
     2161 agagccgccg ggaaaagatc ttcgctgctg gggatttaat ctccggtccc cgttcggtca
     2221 tcgaatccat ggcccagggg agggaagcgg cagtctccat cgaccgtttc ctccagggag
     2281 aaacgctgag gtggggccgg gcctactggg acggcacctg cattaccgac tttcccgtcg
     2341 atcgatccgg agccatcgtg cgccccaggg cctcacttcc ccggcttccg gttcaatccc
     2401 gcaatgcctt gaacgagata gaaaaggcat tgggaacgaa ggaagcccgc gaagaagccg
     2461 aacggtgcct gaactgcggc cggccggggg aagtcaacca aacttgctgg tattgcctcc
     2521 cgtgcgagat cgaatgtccg gtggatgcac tagaggtccg tttgc
//
LOCUS       DASPRR010000449         4352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2200066, whole genome shotgun sequence.
ACCESSION   DASPRR010000449 DASPRR010000000
VERSION     DASPRR010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200066"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..620)
                     /locus_tag="VEL68_16480"
     CDS             complement(189..620)
                     /locus_tag="VEL68_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXZ37620.1"
                     /translation="MDAEIGPSIYWDGSAVLSALVKDSHSDAALNWIQKEGLHFVSTL
                     AYAEVFAVLDRMERERILTKVLVQSAVQAFIEGPWRFLTLGPAREQMDSLRGKYSLRG
                     ADFWHLSLAKTLKREIPDLIILSFDGKLKGVAEKEGFPSPK"
     gene            complement(620..907)
                     /locus_tag="VEL68_16485"
     CDS             complement(620..907)
                     /locus_tag="VEL68_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXZ37621.1"
                     /translation="MATSVGIREAKIHLSHLLKKVRRGMEIVITERGKPVGKIIPLRR
                     SPLSLAERITELEMRGLIEEKKSPSRPLPPPIPVEGDVAQRFLQQNRQGRL"
     gene            complement(1078..1992)
                     /locus_tag="VEL68_16490"
     CDS             complement(1078..1992)
                     /locus_tag="VEL68_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXZ37622.1"
                     /translation="MSKVRKIEKIWKSRATIEGAGVHLKRAFGFQEVPRLDPFLLLDD
                     FHSNKPAEYLAGFPWHPHRGIETITYVLHGEVEHGDSMGNKGIISPGDVQWMTAGSGI
                     IHQEMPKGAPSGLMWGFQLWANLPASQKMMDPRYRDVKQSQIPEIALDSGVKVKIICG
                     RVNKVSGPVKDIVIEPEYLDVHVPPKTAFTHPVKTGHTAFAYLIEGKGYFDQERDSFK
                     HDVVGANYFDFQRECLLGPENLALYERAGDQIRVTTEESSVRFLLVSGKSIGEPVAWY
                     GPIVMNTQKELRIAFDEYQKGTFIKHKG"
     gene            complement(2239..2616)
                     /locus_tag="VEL68_16495"
     CDS             complement(2239..2616)
                     /locus_tag="VEL68_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37623.1"
                     /translation="MRVQLADSDRLGCLLERLEIRPTQGCLLQIDPLWIEKEIRYLGE
                     PLKIIEIEKFQGKAILRSFPPRRENGTVHFFELVIDPREGLSFNRLSYDRTLGSRTQT
                     PVPLTRETLERLLADLVSLASKD"
     gene            complement(2911..>4352)
                     /locus_tag="VEL68_16500"
     CDS             complement(2911..>4352)
                     /locus_tag="VEL68_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HXZ37624.1"
                     /translation="GLSKIYEMVINNDPCYAYLLKCNNLIDQKLVMAHVYAHCDFFKN
                     NLYFAPTNRKMIDEMANHATRVQRYVDRFGSQAVEAFIDCCLSLENLVDPHAPFIKRR
                     EEEEKADRAFLKSLGSDHEHDPFHCGPKSSFSYSSTGCTPGAGMDSGSPVPEGGQKED
                     LFADDEEEERIGPRRLKSKGYMDRYVNPPEFLRSRAEELKAKRKEKKKFPESAEPDIL
                     LFLIENAPLKNWQRDILSIVREEAYYFIPQGQTKIMNEGWATYWHSRLLTEKVLNDSE
                     VVDYADHHAGTLGGSKFHLNPYKLGVELFRDIEERWNKGRFGKEYEECDELGAREKWD
                     KKLGLGKEKVFEVRRLYNDLTFIDTFLTEDFCRQQKLFVYGKDEAGQAWVIQNREFSQ
                     VKKALLESLTNLGQPIIRAVDGNFDNRGELLLSHQHEGRDLKWDFSRDTLVNLHRVWK
                     RPVHVETQREGRKTRLTFDGKEHRERHLD"
BASE COUNT          943 a         1145 c         1142 g         1122 t
ORIGIN      
        1 ccagagggag ggcgatgccc aaattctccg cttcgatggg cagattgccg tatagaaaag
       61 aatccccgag aatggagagg ccgatggtca acccgacgat gaataccagg cgttggggtt
      121 tttggggggg gatcgaaggg gtcattcggg gcaactgagc aaattttatt ccttgcgatg
      181 gggcgctttc atttcggtga aggaaagcct tctttttccg ccaccccttt caatttccca
      241 tcaaaagata aaatgattaa atccgggatt tcccttttta acgtctttgc cagagataag
      301 tgccagaagt cagcacccct caaagaatat ttcccccgca gggaatccat ctgctcccga
      361 gccgggccca gagtcaggaa acgccaagga ccttcgataa atgcctggac cgccgattga
      421 accaggacct tcgttaatat cctctccctt tccattcgat cgaggacggc aaagacttcg
      481 gcataggcca aggtagaaac aaaatgaagt ccttccttct gaatccagtt caacgccgca
      541 tcactgtggg agtctttcac cagagcggat agcaccgccg atccatccca ataaatggag
      601 ggtccgattt ctgcatccat cagagtctcc cctggcggtt ttgctgcagg aatctttgag
      661 cgacatcgcc ttccacaggg atcggcggcg gtaggggtct ggaaggcgat ttcttttctt
      721 caataagacc ccgcatttcc aactcagtga ttctttcggc caaagaaaga ggggaacgcc
      781 ggagaggaat gattttcccc accggtttgc ccctttcggt gatgacgatt tccatccctc
      841 tgcgaacctt tttcagcaaa tggctcaaat gaattttcgc ttcccggatt cccaccgatg
      901 tagccatatc tttcacctgc cgcgaatagc tattgtggtc tctttataat gtggttattg
      961 tagttctttt attcattttg ctcaataaaa ttcaaaaaaa tacccatggt aagaactaat
     1021 gaatatcctt cgcaggctaa tgcttgttcc aaggaatcct ttcgaagtta cttggctcta
     1081 tcctttgtgt tttatgaaag tccccttctg gtattcatcg aaagctattc gcaactcttt
     1141 ctgggtgttc atgacgatgg gcccgtacca ggcgacgggc tctccgatgg atttgccgga
     1201 taccaggagg aagcggaccg aggattcttc ggtggtgacc ctgatttgat ctccggcgcg
     1261 ctcatagaga gccagattct ccgggcccaa gaggcactct ctctggaagt caaaataatt
     1321 ggcccccacc acgtcatgct tgaaggaatc cctctcctgg tcgaaatacc cctttccttc
     1381 gatgaggtag gcgaaagccg tgtgcccggt tttcaccgga tgggtaaagg cggttttggg
     1441 gggtacatgg acatccaaat actccggttc gatgacgatg tctttcaccg ggcctgaaac
     1501 cttattcacc ctgccgcaga tgatcttcac cttcaccccc gagtctagtg cgatctccgg
     1561 aatctggctc tgtttcacgt cccggtagcg cgggtccatc atcttttggg aagccgggag
     1621 attggcccaa agctggaacc cccacatgag tcccgagggg gcccccttgg gcatctcctg
     1681 atggatgatg ccgctgccag cggtcatcca ctgaacatcc ccgggggaaa tgatcccttt
     1741 gttccccatg ctgtccccgt gctccacttc cccatggagg acataggtga tggtctcgat
     1801 cccccggtga ggatgccagg ggaatccggc taggtattcc gccggcttgt tcgaatggaa
     1861 gtcatccagg aggagaaagg ggtccagccg aggtacttcc tggaagccaa acgcccgttt
     1921 gagatgcacc cccgcccctt caatcgtcgc cctgcttttc cagatctttt caatttttct
     1981 cactttgctc atggccccct ccccttttta gagtttaccc tccaataggt catattttta
     2041 aaatactaga aagagatcga atagcaagtg atttagaggg agcagcgata taaaagagcg
     2101 ggatccaacg gtggacgcag agaggaatca gaaaggaaag cccgaaatga actggaactg
     2161 ggattgaagg atggcggttg aacaagtacc cccaaggggg gtctttgtta gaaggaaagg
     2221 aggtcctgct ttactgcttc agtctttcga agcgaggctc accaagtccg ccaagaggcg
     2281 ctccagagtc tcccgggtca ggggaaccgg ggtctgggtg cgagacccga gggtccggtc
     2341 ataggataac cgattgaatg agagaccttc cctagggtcg atgaccagtt cgaagaaatg
     2401 aacggtcccg ttttctcttc ggggggggaa actcctcaga atagcttttc cttggaactt
     2461 ttctatttcg atgattttca ggggctcacc gaggtaacga atttcctttt caatccagag
     2521 cgggtcgatc tgcaacaaac agccttgggt ggggcgaatc tccagccttt caagaagaca
     2581 tcccagccgg tccgaatcgg cgagttggac ccgcacttgg agactcccag ctgaagccag
     2641 gatttgttta ttggattccc atggaccttt tcgcacctgc tccattatat tttcagcgct
     2701 ggcgatcatc tttctattgt ttccttcccg tcttcccctt tttggagaaa accgtaattt
     2761 cataaatatt atcggcccat tcgaaggcat tctttaaccc tcctttctac atctttcttc
     2821 ttttcatgtt cagcgggaaa ccctctatat cctggcggga cagggcgagg gggaggggga
     2881 aaaggggggt tccaggtgtg tgcaggttaa tcaatccaga tgtctttccc tgtgctcctt
     2941 gccgtcgaag gtaaggcggg ttttccggcc ttctctttgg gtttccacat ggaccggtcg
     3001 tttccatacg cgatgaaggt tgactagggt atcccgagaa aaatcccatt tgaggtctct
     3061 gccctcgtgc tggtgggaga ggagaagttc tccgcgatta tcgaagttcc cgtccactgc
     3121 ccggataatg ggttgaccta ggttggtcag actctccagg agggcctttt taacttggga
     3181 gaactcccgg ttctggatca cccaggcttg gccggcttcg tctttgccgt atacgaacag
     3241 cttctgctga cggcaaaaat cctcggtaag gaaggtatcg atgaaggtca ggtcattgta
     3301 taaccggcgc acctcgaaga ccttttcctt tcccaacccc aactttttgt cccatttttc
     3361 ccgcgcccct aattcgtcgc attcctcata ctcttttccg aaccggccct tgttccagcg
     3421 ttcctcaatg tcccggaaga gttccacccc cagcttgtag gggttaagat ggaacttgga
     3481 ccctcccagg gtgcccgcgt ggtggtcggc gtaatctacg acctcggaat cgtttaggac
     3541 cttctcggtc aggagccggg agtgccagta ggtggcccat ccctcattca tgattttggt
     3601 ctgaccctgg gggatgaagt aataggcctc ttcccggacg atggagagta tgtcccgctg
     3661 ccagtttttc agtggggcgt tctcgatcaa gaacagaagg atatcgggct cggcggattc
     3721 gggaaatttt ttcttttctt tccgcttggc tttcaattcc tcggcccggg agcggaggaa
     3781 ttcgggggga ttgacgtagc ggtccatata tcccttgctt ttgagccggc ggggaccgat
     3841 ccgttcttct tcctcgtcat cggcaaaaag gtcttccttc tgacccccct ccggcaccgg
     3901 tgagcctgag tccatcccgg cccccggggt acaaccggtg gaggaataag aaaaggaaga
     3961 tttcggcccg caatggaatg gatcgtgttc atgatccgac cccagggatt tcaagaaggc
     4021 ccgatcggct ttttcttctt cttctcgcct ttttatgaag ggggcgtggg gatcgaccaa
     4081 gttctccagg gataggcaac agtcgatgaa ggcctccacc gcctggctgc cgaagcgatc
     4141 cacataccgc tggacccggg tggcgtggtt ggccatctcg tcaatcatct tccggttggt
     4201 gggggcgaaa tagaggttgt tcttgaaaaa atcgcagtgg gcataaacat gggccatcac
     4261 cagcttctgg tcgatcaggt tgttgcactt gaggaggtag gcgtagcagg ggtcgttgtt
     4321 gatcaccatt tcatagattt tggaaaggcc at
//
LOCUS       DASPRR010000450         8312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1721874, whole genome shotgun sequence.
ACCESSION   DASPRR010000450 DASPRR010000000
VERSION     DASPRR010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721874"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..608
                     /locus_tag="VEL68_16505"
     CDS             300..608
                     /locus_tag="VEL68_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ37625.1"
                     /translation="MREKVLSMDQAAGWVQDGTLLGFTSQSVKNSPMAFLRELVRRKI
                     RNLRMATLTGGGLYTDLLIGAGAVAEYETCHCSLGDYGPAPNFQRALRLRSVKMKDTT
                     "
     gene            615..1160
                     /locus_tag="VEL68_16510"
     CDS             615..1160
                     /locus_tag="VEL68_16510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ37626.1"
                     /translation="MYSMVQAGSMGLPYIAVRGLLGSDLLKYRADLKLQQNPFNSGEE
                     VAVAQPIRPEVAVFHALKADRWGNAVTPGLRDDLTMARASRRVVVTAEEIVDGELSPR
                     DALANTFLPALDVDGVVHAPFGSHPCSCGSSYPVDDRLVKEYLDAAKSEETFRAYLEK
                     YVFSVSNHQEYLQKVGLAAVA"
     gene            1175..1921
                     /locus_tag="VEL68_16515"
     CDS             1175..1921
                     /locus_tag="VEL68_16515"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXZ37627.1"
                     /translation="MTETVSAYTPEELMAVVISREVRDFETVGVGAASPIPASGCILA
                     EQIQAPHINLIILGSSEYYPFPGGGSELHFLAQRGDLDLFFLSGIQIDSLGNINLHVI
                     GDYRSPQMRLPGAYGSAMLYYMAHRVVLFRTEHTKRTFVEKVDFVTAPGITPANVHRE
                     GGPTKVVTPKALLAWDRKVGRWLLESVHPGSSLEEVKENTGFELGMLNSLSMTPPPTE
                     KELSTLRTVVREKLKKVYPDFAEKKIKGPN"
     gene            complement(2150..3505)
                     /locus_tag="VEL68_16520"
     CDS             complement(2150..3505)
                     /locus_tag="VEL68_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3371..3373),aa:Sec)
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ37628.1"
                     /translation="MAKERLIVIGGNAAGMSAASQAGRQKPTLEILAFERSPHTSYSA
                     UAVPYYIGRVVDDVKRLVVRSPEAFREKQGIDARVFQKVSEIDLRQKQVQGEDLKTGK
                     SWKEPFDQLLIATGAASIRPPIPGIDAKGVYELNTLQSGIEARRAVDEKNPRHIVIIG
                     GGYIGLEMAENFIHRGIGVTVVEKTPQVMNTLDPDMAALLIKPLEEAGVKLYLNESLK
                     ALESRGGEVREAVTDKRSLPADLVVLGLGVRPDSAIAREAGIPLGKTEGIKVNDRMQT
                     EVEGIWAAGNCVESFHLVSRRPFYIALGTVANKQGRVAGINIAGGRAVFPGVVGTAMV
                     KVFSLEVARTGLQEKEIQELGLSFVTAKIDAHTRARYYPGTGPLTVKILAEKGTGRLL
                     GGQIVGREGAGKRIDTLAAALHARLTVEDLINLDLGYSPPFSPVWDPVQIAARQAAGQ
                     V"
     gene            complement(3556..4068)
                     /locus_tag="VEL68_16525"
     CDS             complement(3556..4068)
                     /locus_tag="VEL68_16525"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXZ37629.1"
                     /translation="MTKPGKIKGEGFTRALRELSEAKVPHRVMTFKAEEKSAEEVTRE
                     TGFPLAQVVKTLLVRGASQKYYLALCPGDRQVNLKALSKAVNEKTVDMAKREEVPKIT
                     GYFIGGVSPIGTHRPLPVLMEESILAMPELAISAGQWGCQAVLRPSDLRQFLGSRVTI
                     VSFAQAERNG"
     gene            complement(4207..5373)
                     /locus_tag="VEL68_16530"
     CDS             complement(4207..5373)
                     /locus_tag="VEL68_16530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXZ37630.1"
                     /translation="MEGPENKELVRILRDRIRARGQVTFAEFIDLVLYHPAEGYYHSS
                     REKTGPGGDYYTSPHIHPIFGQLLARQVHQMWEILGHPAPFTLVEMGGGRGLLCSDIL
                     HYCRSQFGDFYRSLDYILAEISSPSVEKQRSLLSPFLNERKVEFLDPEELWNRPQGFI
                     GCFLSNELIDAFPVHLVQQKDGKLWEIYVAWGDESFKEVLGPPSNPALEEYFFLYGSP
                     LEEGQRAEVNLKALEWIERVSKVLRRGFILTVDYGYEAPELYQPTRRDGTLLCYFRHT
                     TSSNPYERIGSQDITAHVNFTALINKGEALGLNKVGYTEQYKFLVSLGLLQDLESLER
                     DSPSHSDPGFLKSKLAMRNFLIPGGMGTLFKVLCQSKGIGEVKLLGFQDPFRQL"
     gene            5519..6121
                     /locus_tag="VEL68_16535"
     CDS             5519..6121
                     /locus_tag="VEL68_16535"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXZ37631.1"
                     /translation="MRRKEFEVQDRERMEEVLKKADIGYLAFNGPDGWPRITALNFFY
                     DGRILWHGAVAGERFECLKKDPRATFMAVSLQMYLPSHLLSEENATSATSAFKSVHVR
                     GRCQIIEDPEEKCAVLNRLMEKYQPEGRFKEITPADPLYAKVLKATGVYALTVEDMSG
                     KFKFGQNKSKEDRQKIAGFLKERGTPLDLQVSLEIQKTLE"
     gene            6298..8253
                     /gene="ftsH"
                     /locus_tag="VEL68_16540"
     CDS             6298..8253
                     /gene="ftsH"
                     /locus_tag="VEL68_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXZ37632.1"
                     /translation="MSEDDERNPSEDFKKKLKSFFSPPDPQRQKQGLPPKAHFSIWYF
                     LIIFLLITVVQNYFLTPKVETIPYSKFKQELVQGHVNNLVIGPDRINGKLKGAGEKGE
                     QDFVTVRVEDPNLIKELDDRKVSYSGLYESKLLSNVLSWVIPLVIFFFIWRFAMKRMG
                     PGYGVMSFSKSKAKVFAEHETKVTFDDVAGIDEAKEELQEVVEFLKNPGKFQKLGGRI
                     PKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSISGSEFVEMFVGVGAARVRDLFSQASG
                     HAPCIIFIDELDALGKARGINVMGGHDEREQTLNQLLVEMDGFESNKGVIIMAATNRP
                     EILDPALLRPGRFDRQVLVDRPDINGREAILKIHSKNVILGPDVDLKKIAARTPGFVG
                     ADLANLVNEAALLAARKNKDQVGSGEFDEAIDRVVAGLEKKKRVMSPKEKEIVAFHES
                     GHAIVAESVEHADPVHKITIIPRGIAALGYTQQQPTEDRYLMTKSELLDRMAVLLGGR
                     VAEELIFGEISTGAQNDLQRATDMARSMVTEYGMSERLGLVTYERERRPMFLPESFGP
                     SKVYSEEKAAEIDEEVSKVVEDCHQRVRRILGEKREVLNRLARLLLEKEVVQGDEMRA
                     LMGKSGPVPPALPRGPETSLPPQQPAA"
BASE COUNT         1884 a         2317 c         2392 g         1719 t
ORIGIN      
        1 cgtcattccg gcgaaagccg gaatccagtg ttcgatttca ttcaattaat ttgaatcctg
       61 gatgccggtc ggagactcga ctctacgagc ttccgccggc atgacgaatt acgacacagt
      121 ctcttggagg gggagggagg gtgggggaaa aaattcacgg gcggcgaggc gcttgcaagg
      181 acacgatgcc tcgtgcccct acgaagctcc ccatctgatg catcttgaca taaaggcgaa
      241 taaaggctac agatgaagca taaaaagaaa tctactggtc tcaaggagat atgatcacca
      301 tgagagaaaa agtcctttct atggaccaag cagcggggtg ggtgcaggac ggaaccctcc
      361 tcggttttac ttcccagtcg gtaaaaaact cccccatggc ttttctccgt gaattggttc
      421 gccgcaaaat ccggaacctg aggatggcca cccttacggg aggcggcctc tacacggacc
      481 ttttgatcgg ggccggggct gtggcggagt atgagacctg tcactgctcc ctgggcgatt
      541 acggcccggc ccccaatttc cagagggcct tgcgcctccg gtcggtcaag atgaaagaca
      601 cgacctgagg ggtgatgtat tccatggtcc aggctgggtc catgggcctt ccctacatcg
      661 cggtccgcgg ccttctgggg agcgatctcc tgaagtaccg cgccgacctc aagttgcaac
      721 agaatccgtt caactccgga gaagaagtgg cggtggccca acccatccgg ccagaggtgg
      781 cggtatttca cgccctgaaa gccgaccgct ggggtaatgc cgtcactccg gggctgcggg
      841 atgacctgac catggcccgt gcttcccggc gggtggtcgt aacggcagaa gagatcgtgg
      901 acggggagct gtcccctcgc gatgccctcg ccaacacttt tctgccggcc ctcgacgtgg
      961 acggggtggt tcacgcgcct tttggttcgc acccctgcag ctgcggaagt tcctatccgg
     1021 tggatgaccg gctcgtaaag gaatacctgg atgcggccaa aagcgaagag accttccggg
     1081 cttacttgga aaagtacgta ttctccgtga gcaaccacca ggagtacctg cagaaggtgg
     1141 ggctggcggc ggtcgcttag gaggaagaac catcatgact gagacggttt ccgcttatac
     1201 cccggaggaa ttgatggccg tggtcatctc ccgggaagtg agggatttcg agactgtcgg
     1261 cgtaggggct gcctctccca ttccagcctc gggatgcatc ctggccgagc agatccaggc
     1321 tccgcatatc aacctgatca tcctgggaag cagcgaatac tatccttttc ccgggggcgg
     1381 gagcgagctg cacttcctcg ctcagcgggg ggacctggac ctcttcttcc taagtggaat
     1441 ccagatcgat agcctgggga acatcaacct tcatgtcatc ggagattacc ggtcgccgca
     1501 aatgagactt ccgggggcct atgggtccgc catgctttat tacatggccc accgggtcgt
     1561 cctcttccgc accgaacaca cgaagcgcac ctttgtagaa aaagtggatt tcgtcaccgc
     1621 tccggggatc acgccagcca acgtgcaccg ggagggaggc cccaccaaag tggtgacccc
     1681 aaaagcactt ttggcctggg atcgtaaggt aggaagatgg cttctggaat ccgttcaccc
     1741 cggatcgtcc ctggaggaag taaaggagaa cacagggttt gaattgggga tgttgaactc
     1801 ccttagcatg acccctcccc ccaccgaaaa ggaactttcc accctccgga ccgtggttcg
     1861 ggaaaagctc aagaaggtct atccggactt cgcggagaaa aaaatcaagg gcccaaatta
     1921 gccgcagaga acgcagaaga aagtttccac ccctgaaaaa cccctctccc cttggagact
     1981 gtgtcgtaat ccctcgttcg cccttcgaca ggctcagggc gaacggctaa gtgcctgaat
     2041 tttagtgtgc tcataatccg ttcgtggtga gcttgtcgaa ccatgaacgg atcatgacac
     2101 agtctccaag gggagagggt tggggtgagg gggataagaa tagaaatcgc tatacctgcc
     2161 cggcagcctg tctggcggca atctggaccg ggtcccagac cggggaaaag ggcggtgagt
     2221 accccaggtc caggttgatc aaatcctcca cggtaaggcg tgcgtgcagg gcggcggcaa
     2281 gcgtatcaat ccgcttgcca gctccttccc tccccacgat ttggccgccg agaagccttc
     2341 cggtcccttt ttccgcaaga attttaacgg tcagcggccc ggttccgggg taataacggg
     2401 cccgggtgtg agcatcaatt ttagcggtta cgaaggaaag gccgagctcc tgaatttcct
     2461 tctcctgcaa acccgttcgg gccacttcca gggagaagac cttgaccatg gccgttccca
     2521 cgactccagg aaagacagcc cggcccccgg caatattgat tcccgccacc cggccttgtt
     2581 tgttggccac ggttcccaga gcaatgtaga acgggcggcg gctgaccagg tggaaggatt
     2641 caacgcagtt ccccgctgcc cagattccct cgacctcggt ctgcatccgg tcgttcactt
     2701 taatcccctc ggtcttgccc aggggaatcc cggcttcccg ggctatggcg gaatccggcc
     2761 ggactcccag tcccaggacc accaaatcgg caggcaggct gcgcttgtcg gtgaccgcct
     2821 ctcggacctc ccccccgcgg ctctccaagg ccttcagcga ttcattcagg taaagtttca
     2881 ccccggcttc ctccaggggc ttgatcaaga gggcggccat gtccgggtcc agggtgttca
     2941 tgacctgggg ggttttctca acaacggtca caccgattcc tcggtggatg aagttttcgg
     3001 ccatctccag cccgatatag cctcctccga tgatcacgat atgacgaggg ttcttttcat
     3061 ccaccgcccg gcgagcctcg atcccgcttt gtagggtatt cagctcataa acccccttgg
     3121 catcgatgcc gggaatcggc ggtcgaatgg aggccgcacc cgtagcgata aggagctgat
     3181 caaagggctc tttccaggat tttccggttt tcaggtcttc tccctgcacc tgcttctgac
     3241 ggagatcgat ttccgaaacc ttctggaaaa cccgggcgtc gattccctgc ttttcccgaa
     3301 aagcttccgg ggatcggacc accaggcgct ttacgtcatc gacaaccctg ccgatgtagt
     3361 agggaaccgc tcaggcagag taagacgtat gagggctccg ttcaaaggca aggatctcga
     3421 gcgtgggttt ctgacggccg gcctgcgaag cagcactcat tccggctgcg ttgccgccga
     3481 ttacgatcag gcgttctttg gccataggta agcacctcta tttaagatat atcgggatcg
     3541 ttttacatcc cgattttagc cgttgcgttc cgcttgagca aaagagacaa tggtcacccg
     3601 actcccgaga aattgtcgca aatcggatgg tcgaaggaca gcctggcaac cccattgccc
     3661 ggcgctgatg gccaactcgg gcatggctaa gatggattcc tccataagca ccgggagggg
     3721 gcggtgggtt ccgatggggc tgactccgcc gatgaagtat cctgtaatct ttggcacctc
     3781 ttcccgtttg gccatatcca ccgtcttttc gtttacggcc ttggagagtg ctttcaagtt
     3841 cacctgccgg tcccccggac acagggccag atagtatttt tgggaagctc cccgaaccaa
     3901 aagggtctta accacttgcg ccaggggaaa accagtttcc cgggtgactt cctccgccga
     3961 tttttcctcc gccttaaagg tcatcacccg atgcgggacc ttcgcttcag acaattctct
     4021 cagggcccgg gtaaaaccct ctcctttgat cttacctggt ttggtcatgt ctctttcata
     4081 tcaatgtatc tttttcccat cacccggttc aaggtgataa taatagtttc aggttgcagg
     4141 ttttgagttt caggttagga atattcaaga tcgagacctt gaaacctgaa aaccgcaacc
     4201 tgaaactcag agttggcgga atggatcctg gaaacccaag agtttcactt ccccaatccc
     4261 tttgctctgg cacaaaactt tgaaaagggt ccccatcccc ccggggatca gaaagtttct
     4321 catggccagt ttgctcttca ggaagcctgg atcggaatga ctcggcgaat ccctctccag
     4381 gctttccagg tcctggagca atccgaggga cactaagaat ttgtattgtt ccgtatagcc
     4441 gactttattt agacctaggg cttccccttt attgatcaaa gcggtgaagt tcacatgggc
     4501 ggttatatct tggctcccga ttcgctcata gggattggaa gaggtagtgt ggcgaaaata
     4561 acaaagcaac gtcccatccc ttcgggtcgg ctggtaaagc tcaggagctt cgtacccata
     4621 atcgacggtc agaataaatc cccttcggag gaccttgctg accctctcga tccattccaa
     4681 ggccttcaag tttacctctg cccgctgacc ttcttccagc ggggacccgt agaggaaaaa
     4741 atattcttcc agggccggat tggaaggagg acccaggacc tctttaaagg actcatcccc
     4801 ccaggccaca taaatttccc ataactttcc atctttttgc tggacgaggt gaacaggaaa
     4861 ggcgtcaatc aattcgttgg agaggaagca accgatgaaa ccctggggtc tgttccaaag
     4921 ctcttccggg tccaggaatt ccaccttcct ttcgtttaga aaaggagata ggagggaccg
     4981 ctgcttttct accgagggcg aactgatttc cgccaggata taatccaggc ttcggtagaa
     5041 atctccaaac tgagagcgac aataatggag aatgtccgaa cagagaagac ccctccctcc
     5101 gcccatctcc acaagggtaa acggggcagg atgaccgagg atttcccaca tctggtggac
     5161 ctggcgggca agaagctgtc caaagatcgg atgaatgtgg ggactggtat aatagtcccc
     5221 ccctggcccg gtcttttccc gggaggaatg ataataaccc tcggctggat ggtaaagaac
     5281 caggtctata aactcagcaa aggtgacttg gccacgggcc cggatgcggt cccggagaat
     5341 cctgactaat tccttatttt ccggcccttc catgtgtggt attataaccc actttcatgg
     5401 gaacacgttt caagtttcag gttccgggtt ttcgcttcgg ggccttgcca gacagaagat
     5461 tttttgttct ggaaagggca cctggcatcg ggcaaacggg aacttttttg gaggagtcat
     5521 gaggagaaaa gaattcgagg tccaggatcg ggagcggatg gaagaggtcc tgaagaaggc
     5581 ggatatcggg tacctggctt tcaacgggcc ggacggctgg ccaaggatca ccgcgctgaa
     5641 ttttttctac gatggacgga ttctatggca cggagctgta gccggggagc gcttcgaatg
     5701 cctgaaaaaa gacccgcggg ccacttttat ggccgtttcc ctgcagatgt acctcccctc
     5761 ccacctcctc tcggaagaga atgccacctc ggccacctcg gccttcaaat cggtccacgt
     5821 gcggggtcgc tgccagatca tcgaagaccc ggaagagaaa tgcgccgtcc tcaaccggct
     5881 catggagaag tatcaacccg agggaaggtt caaggagatt actcccgcag accctctcta
     5941 tgccaaggtc ctgaaggcca ccggcgttta tgctttgacc gtggaagaca tgtcaggaaa
     6001 attcaaattc ggccagaaca aatccaagga agaccggcag aagatcgccg gttttctgaa
     6061 ggagcgcggg acccccttgg acctgcaagt ctccctggaa atccagaaaa cattggaata
     6121 gcagaagtat aattacaggt cggcgacaag ggggggacgg aagcggcggg aaatgaaagg
     6181 aaaccggcgg cgccgccggg gccccggaga tttgaatctc gcttccataa agggtaaaat
     6241 taagaatgag cttcttttat tgaaccggcc tgagcctttt cggccaagga ggcccgcatg
     6301 tctgaagacg acgagaggaa tccgagcgag gatttcaaga aaaaattgaa atccttcttt
     6361 tccccgcccg acccccaaag gcaaaagcag ggtctgcccc cgaaggccca cttcagcatc
     6421 tggtatttcc tgatcatctt cctgttaatc accgtggtgc agaattattt tctgaccccc
     6481 aaggtagaga ctatccccta cagcaagttc aagcaggaat tggtccaggg gcacgtcaac
     6541 aacctggtca tcggcccgga ccggatcaac ggaaagctga agggggcggg ggaaaaggga
     6601 gagcaggact ttgtcaccgt ccgggtggag gaccccaacc tgatcaaaga actggacgac
     6661 cgcaaagtca gctacagcgg cctctacgag agcaagttgc tgagcaatgt cctatcgtgg
     6721 gtgattccgc tggtcatctt cttcttcatc tggcggttcg ccatgaagcg gatggggccg
     6781 gggtacgggg tcatgtcttt cagcaagagc aaggccaaag tcttcgccga acatgagacc
     6841 aaggttacct tcgacgacgt ggccgggatt gacgaggcca aggaagagct ccaggaagtg
     6901 gtggagttcc tcaagaatcc ggggaaattc cagaagcttg gggggagaat ccccaagggt
     6961 gttctcctcg tcggcccccc cgggaccgga aaaacccttc tggccaaagc ggtcgccggg
     7021 gaagccggcg ttcccttctt cagcatcagc gggtccgaat tcgtggagat gttcgtgggt
     7081 gtcggggcgg cgagggtccg cgacctcttc tcccaagcca gcgggcatgc cccctgcatc
     7141 atcttcatcg acgaactgga cgccctgggg aaggcccggg ggatcaacgt catgggaggc
     7201 catgacgagc gggaacagac gctcaaccag cttctggtgg agatggacgg gttcgaatcg
     7261 aacaaagggg tcatcatcat ggccgccaca aaccggccag agatcctgga cccggcgctc
     7321 ctgcgcccgg gccgattcga ccgacaggtc ttggtggacc ggccggacat caacgggcgg
     7381 gaggcgattc tgaaaatcca ttccaagaat gtaatcctgg gtccggacgt ggacctaaaa
     7441 aaaatcgccg cccggacccc cggtttcgtg ggggccgacc tggccaactt ggtaaatgag
     7501 gcggcgctcc tggcggcgcg gaagaataaa gaccaggtgg gatcagggga gttcgacgaa
     7561 gccatcgacc gggtggtggc cgggttggag aagaaaaaac gggtcatgag ccccaaggag
     7621 aaggagatcg ttgccttcca tgaatccggc cacgccattg tagcggaatc cgtggagcac
     7681 gcggacccgg tgcacaagat caccatcatc ccgaggggca tcgcagccct ggggtacacc
     7741 cagcagcagc cgacggaaga ccgctacctg atgaccaaat cggagctgct cgaccgtatg
     7801 gcggttctct tggggggaag agtggccgaa gaactgatct ttggagagat ctcgaccggg
     7861 gcgcagaacg acctgcagcg ggctacggac atggcccgtt ccatggtcac ggaatatggg
     7921 atgagtgagc gcctgggcct ggtcacctac gagcgggaaa ggagacctat gttcctcccg
     7981 gagagtttcg gccccagcaa ggtctacagc gaggagaagg cggccgaaat cgacgaggag
     8041 gtctcgaagg tcgtcgagga ctgccatcaa agggtgcgac ggatcctggg cgagaagcgg
     8101 gaagtgctca atagactggc caggctcttg ctggaaaaag aagtcgtcca gggagacgag
     8161 atgagggccc tcatggggaa gtccggcccg gttcctccag ccttgcccag gggcccggaa
     8221 acaagcctac ctcctcagca gccagcggcc taatccctcc gccaggcggg agacgttttc
     8281 cggacttcgg agcaggagac ttaagtacga ta
//
LOCUS       DASPRR010000451        11750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1234583, whole genome shotgun sequence.
ACCESSION   DASPRR010000451 DASPRR010000000
VERSION     DASPRR010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234583"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..1801)
                     /locus_tag="VEL68_16545"
     CDS             complement(362..1801)
                     /locus_tag="VEL68_16545"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ37633.1"
                     /translation="MIKHPLHQIMHPKSVAVVGASNDLFKMGSIQLANLLGGKYPGKV
                     FPIHPQQDPILGLKAYRSGRELPEPVDLAIITIPPSAVPEVLKDLGERGVKRAIIISG
                     GFKELGEKGRALEEQALQIARQYGIRFLGPNCIGVINPAFHLNPTMYPYIHQPGAVAV
                     ASQSGTYITQVLPLLAKSQIGYSKAFSVGNGADLDLVDCLEYLGGDSETRAIALYIEG
                     IRRPRDFVRIASRVSEVKPVVALYVGGTEAGARSSASHTGSLSGPDFLYDALFKQSGV
                     IRAYSVEDLYEWAWALASLPLPKGKNMAILTHSGGPATSLADACNRWGLKVPVFSPEL
                     QAKVRQLIPETGSFSNPVDLTFFMDMSVLMEKLPQMILENPSIDGLLLHGIIGSSYVR
                     SVGQLAQEWVKLPSYDAMKGFFLSSIESFARLPEKFGKPVIASSFNEREDDAVAYVQD
                     RRLPCYRAPERGVRAMAALCRYAEVRSRI"
     gene            2025..2603
                     /locus_tag="VEL68_16550"
     CDS             2025..2603
                     /locus_tag="VEL68_16550"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ37634.1"
                     /translation="MKKENAFLFIAIAFVAGIVTGVIATVLYEEKMPVPLPSIKPSQS
                     SPAPANTADIQKQISMLQGILKEDPKNLKALVELGNLYFDAEQFDSAIQYYARALELD
                     PKNPDVRTDMGIMYRRKGDPDRALAEFKKAAQDDPKHVNSRYNMGVVLLHDKADIKGA
                     IKAWEEYLKVEPTGPRAENIRSQIEKMRNMVK"
     gene            complement(3092..4099)
                     /locus_tag="VEL68_16555"
     CDS             complement(3092..4099)
                     /locus_tag="VEL68_16555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXZ37635.1"
                     /translation="MISKASPNPGKTLIIHSGGIGDLLLALPAMRFFRHALRQFPLYL
                     MGHSERLALVAYDLKAETIHSVEQAGMAYFYLDDAFLPSPLCSFFSSFGLILSFSGKG
                     GQTLSKNLEKAGAARVLNLPSFPSEGPKVHVSDYLIELLREKGFEGEVADHPLQLSKE
                     ALNFSRDYLARSGLKENERILAIHPGSGNPAKNWSPSHFAGIADWAARRARVLLISGP
                     AQDGVREVKENLRKAAPLIAENLSLLQLAAILRRCTAYLGNDSGITHLASSLGLPTIA
                     LFGPTDPEVWGPRGTSVRIIFGARPDPPFFSGAPIHRCAPSPINLKPDEVIAVLAPLL
                     G"
     gene            complement(4141..4767)
                     /gene="plsY"
                     /locus_tag="VEL68_16560"
     CDS             complement(4141..4767)
                     /gene="plsY"
                     /locus_tag="VEL68_16560"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXZ37636.1"
                     /translation="MTLLLLIFAYLLGSVPTGVILTRAFSDVDPRTQGSKNIGATNIY
                     RTAGKKLGVLTLVGDILKGLIPVVIARAVLDSHFWIAAVALTAFLGHLYPIFLKFKGG
                     KGIATGLGAFLALATLPAFLSFLVFAAVVYKSRYISLGSLTAATAFPLFLALLNPHHI
                     YIPFAIVIGLFIFWRHRENIQRLMSGKENKFGAKKVTHGVPDADGRSI"
     gene            complement(4945..7179)
                     /locus_tag="VEL68_16565"
     CDS             complement(4945..7179)
                     /locus_tag="VEL68_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ37637.1"
                     /translation="MSEWKKTSCVLCFSNCGLEVTTEGSKILRVRADKENPRSQGYIC
                     RKGAHIAYFQNNPERLKYPLKRVGDRFERISWKQALDEIADKLKEVLERHGPRAVAYM
                     GGGGQGCHFEAAFGVRFLHGLGSKNHYSPLAQELTGYFWVQGKAFGRQYMHPVPDIRG
                     SDFLVFWGANPMVSHDIPAAPVVIRNKKKEPGFMMAVVDPRVTETAKLADIHLPIKPG
                     TDALLLKAMIKILLTEEGLLDREFISAKVNGFETIQNWFDDVNIEENCRVCGLDPGAV
                     REFTRLFATRKSAWRSDLGILMGRHSTLNSYLELILLALAGRIGGKGGNVFGGHLMPM
                     GSHTPEEDPGTWRTVATNIPLIMGVFPPNVMPEEIDNDHPQRVRAVIVSGSNPLRSYA
                     DTAAYERAFAKLDLLVTVEVAMTETAVLSHYVLPARSGYEKWDGTFFQWMYPEYYFDM
                     RRPVVEPDGEQVEEAQVYVELAERLGMIPEYPQKLRELAPDRKAYGIALMQYIQANPQ
                     AASWLPFILAKTLGEELGSKNLSALWGLLARFPLMHPDDVARAGYKPGPFTGEEIFGK
                     ILETPGGVKIGIVDTEKGLSALKTPDKKVLIHFPDMESWVKEVSPASEGAALVNKDYP
                     MILMAGNHMEMVANSIMRDPAWNEGRRYCTMRIHPEDARKLGIADKDLAVIETEAGSA
                     MVEAEVTESSHKGQVVIPHGFGLIHGGRAHGVNVNRLAPARHRDRFAGTPLHRYIPCR
                     VRKA"
     gene            complement(7300..8796)
                     /locus_tag="VEL68_16570"
     CDS             complement(7300..8796)
                     /locus_tag="VEL68_16570"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ37638.1"
                     /translation="MTGKLQYLRRTVQVISFLFLLYLIIQTAFPLEIRIPVDLYLRLD
                     PFIAIITFLTQGEVIMRMLPAFGILLLVIVFGNFFCGWFCPMGATLDFFDRVLFREKK
                     RSKPFDDQPLRRFRYGAFIFAVAAGIMGLQVLYLLDPISLITRTLVVAFYPPAVYIYN
                     HLLPEVQRFLPSNPYILPAMKLPVFKVNLLIFLFFTGVLALGVIRKRFWCRFLCPLGT
                     LFSLTSRLRVVRRSVSEACIQCLKCAKECSVGAIPAETPREYRQQDCISCFKCLDCPP
                     EATVFGFHFPRWKGAEAVSLSRRYVVGSLVLGAFSALAIKTNPLQTESGLKNNRLIRP
                     PGALPEQKFVSVCTGCGKCLKVCPNNALQSTFLEAGLSGLYTPRLVPRIGYCEEHCNF
                     CGRVCPTEAIRPLSVEEKQKVQMGVAKIDKSRCIAYEEGKVCLVCNEQCSYHAIVGDD
                     KKRPIVKEEICTGCGICENKCPVDGESAIIVYTSGVQKKVKPQQETKA"
     gene            complement(8793..9746)
                     /locus_tag="VEL68_16575"
     CDS             complement(8793..9746)
                     /locus_tag="VEL68_16575"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ37639.1"
                     /translation="MDWSSRLNLTRRDFMKGAAAAVALPKMFFLSSPLRPSVAYAAGD
                     ADLIIAQQGTPTQLLQAAMAPLGGMGRFVKKGQRVVIKANIAWARTPEQAATNNPELL
                     SALIKMCYQAGAKRVAVWDHTCDNYQFCFSRSGLKKATQDAGGDLYSGHGRNVYKKLE
                     IPKGKKLKEAEFIQDFLETDVLINFPLPKQHFATELTLALKNTMGVTWDMELLHKIDL
                     HQCIADINTIRKPDLVVVDAIRILTTNGPKGPGKTEDIGEVMAATDMVAVDAHCATYF
                     KHPKTGKPFRPADIQFIKNAYDLGLGEMNLSKIKIKKVKAA"
     gene            complement(9947..11002)
                     /locus_tag="VEL68_16580"
     CDS             complement(9947..11002)
                     /locus_tag="VEL68_16580"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXZ37640.1"
                     /translation="MRQAANVFGMIILSTFLLLSPGFPAEADFIKRPVSFQQLTVKEG
                     LSSDMIFAIAVQGDEVWFGTYAGGTTLYNRSKNTFQVFTTKGEPPEKDDGVAIKWKNH
                     PNYNDVTVILPDKDRLWFGTYFYGFGGGGISYYQPKRKNPWRPFNTNNGLAKKIVSLA
                     MDGDILWVGSERGISALDKKTEKWKVFYSSKDGLSGNFVNALLVQGDFLWAGTNGGVT
                     RLQKSQMKWKNYGQQEGLTDLEIKALAQIGDKIWAGSSQGALYEYDPPAEGWKRIEPT
                     DSLKNGGIYCLAAIQGKVFVCRDNGVSVYGISQNQWDSLTTADGLPSNNVFCATEDKG
                     GVWFGTDQGASLMKQPE"
     gene            11265..>11750
                     /locus_tag="VEL68_16585"
     CDS             11265..>11750
                     /locus_tag="VEL68_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXZ37641.1"
                     /translation="MSGIFGIVSNNNCASTLFYGTDYHSHMGTEYGGMAVLGTRFNRA
                     IHDISQSQFKSKFVDEYKTMQGPMGIGVISDRDPQPLIVGSRFGTFAIVNAGLIENLE
                     SLSTELLEQGHSFGEMTSGGINSVELVAKLINTGKDLVDGFDRMLDKIHGAATLLLLT
                     RE"
BASE COUNT         2730 a         3145 c         3186 g         2689 t
ORIGIN      
        1 tccaggccgt aaaaagcccg cattctttcc tttacctccg cagtcgcctt gggattcaag
       61 tccgtggcca tgtcgatagg ggagcctggg gctaggtgaa tgaccccaaa agaaatgatg
      121 gtgatcccaa tgaagagagg aatcatgagg agaagacgct tgatcagata aaatcccatg
      181 gccttttcac agagaggtct ttttattatc ataaacttgg acggcgaagc gaggcaagag
      241 aaacttccgt gttcaagtca aaaggttgat gacgaggttt tggctgagct gtaagagtta
      301 cgtaaggaag gtttgattct gggttatttc ttcttgctgg tgtgcggaaa tctgggtccc
      361 ctcaaatacg ggagcggacc tctgcatacc ggcacaaagc cgccatggcc cggacccccc
      421 tctccggcgc acggtagcag ggcagtctcc ggtcctggac ataagccacg gcgtcatcct
      481 cccgctcgtt gaaagaagaa gcaatgaccg gcttgccgaa tttctcgggc aatcgggcga
      541 aggattctat agaagaaagg aaaaatccct tcatcgcatc gtaggatggc agcttaaccc
      601 attcttgagc gagttgtcca accgagcgga catagctcga accgatgatt ccgtggagta
      661 acaatccgtc gatggacgga ttttcgagga tcatctgggg cagcttttcc atgagaacgg
      721 acatgtccat gaagaaagtc aggtcgaccg gattggaaaa ggatccggtt tccgggatga
      781 gctggcggac cttcgcctga agttcgggcg aaaagacagg aaccttcagc ccccaacgat
      841 tgcaggcgtc ggctagagag gtagcgggcc cgccggagtg ggtcaaaatg gccatgttct
      901 tccccttggg gaggggaagg gacgccaagg cccaggccca ttcataaagg tcctccacgg
      961 aatacgctcg aatcaccccg gactgcttga agagagcatc gtaaaggaag tccggcccgg
     1021 agagggaacc cgtgtggctg gctgaagagc gggcaccggc ctctgttcct cccacgtaaa
     1081 gggccactac cggctttact tcggataccc gggaagctat ccggacaaaa tcccgggggc
     1141 gcctaatccc ctcgatgtag agggcgatcg cccgggtctc tgaatcaccc cccaggtatt
     1201 cgaggcaatc caccaggtcc agatcggccc cattccccac gctgaaggcc ttgctgtatc
     1261 cgatctggga tttagccagt aaaggcagga cctgggtgat gtaagtcccg ctttgggaag
     1321 ccactgccac agcgcccggc tggtgaatgt agggatacat ggtgggattc aggtggaagg
     1381 ccgggtttat cacgccgatg cagttgggcc cgagaaatcg aatcccgtac tgccgggcaa
     1441 tctgaagggc ctgttcttcc aaggcccttc ccttctcccc caactccttg aatccgccgg
     1501 aaatgatgat cgccctcttt actcctcttt cgccaaggtc cttcaggacc tcaggaaccg
     1561 ctgacggagg aatggttatg atggccaggt caaccggttc cggcaactcc ctgccgctac
     1621 gataagcctt caggcctaga atcgggtcct gctggggatg gatgggaaaa acctttccgg
     1681 ggtatttccc ccccagaagg tttgccagct ggatgcttcc cattttaaat agatcattgg
     1741 aagctcccac cacggccacg gatttagggt gcataatctg gtgcaggggg tgtttaatca
     1801 tggttgtcgc tccccaaata aatgaaagac taagaaatcg tccaaatcct tgagttaccc
     1861 gaccataaca cagataaaat cgttttttca aacgcgcggg aggtttttct cattcgctcc
     1921 ctgccggaga gtgaaaattc ggctctctcc atccatgagc cgatcatttg gcctggatgg
     1981 gctttcgtgc taatatagga aaaattttga gccaggacca aatcatgaaa aaagagaacg
     2041 ccttcttgtt tatcgctatc gcctttgtgg ccgggattgt cacgggggtc atcgctacgg
     2101 ttctctacga agagaaaatg cctgttccgc tcccatccat caaaccgtct caatcatccc
     2161 ccgccccggc caatactgcg gatattcaaa aacagatatc catgctccag ggtatcctga
     2221 aagaagaccc caaaaacctt aaggccctgg tcgagctcgg gaacctttac tttgatgcgg
     2281 aacaatttga ttcagccatc caatactatg ccagggcttt agaactcgac cccaaaaatc
     2341 ccgatgttcg gacggatatg ggaattatgt accggagaaa gggagacccg gaccgggccc
     2401 ttgcggaatt caaaaaggct gcccaggatg accccaagca tgtgaatagc cgttacaata
     2461 tgggtgtggt cctcctacac gacaaggcgg atatcaaggg agcaatcaaa gcctgggagg
     2521 aatacctgaa agtggaaccc accgggccac gggctgagaa tattcgcagc cagatagaaa
     2581 agatgaggaa tatggttaaa tagacacaat tgttcaatcc ttgaggcgga aaatatacat
     2641 ttttgacctt ttacggcagt cgcgatccat atcgggggcc cggctcgagg gaaccaatcc
     2701 acctgtaata atttgaattt atttaataat ttataattaa tcgggttgtt ttggaaatct
     2761 cttccttttc gcttttcctt tggcacggga aatgctcatt tgaatgccct gaaaggatgg
     2821 gcttgggcca tgaagaggaa aaaattttca gaacggatta tcgttattct catttttcta
     2881 ttttttgcca ttcccgtatg cgccctggca ttaactgaat cggctacaac ttggccaagg
     2941 tgctcgagtt catttgttta taatctgata tttcgataat ctgcaagaaa aggttgcttt
     3001 ttctaaaccg ctggattggc tgtcgtttgc tggtccagcg ttttttttta ccttcaaaag
     3061 gacctatccg gcaagcaaaa tcagcgggag attatcctaa gagcggagca agcaccgcga
     3121 ttacttcatc gggtttaagg ttaatcgggg aaggggcgca tcgatggatc ggggctcctg
     3181 agaaaaatgg cggatcgggc ctcgcgccaa aaatgatccg tacggaagta cccctagggc
     3241 cccagacctc cgggtcggtt gggccgaaga gggctatcgt cggcaaaccc aaggaggaag
     3301 ccagatgggt aattcccgag tcattcccca agtaggccgt acaccttctc aaaatggcag
     3361 caagttgaag caaagacagg ttctcggcga tcaaaggtgc agcttttctc aaattttctt
     3421 tcacttcccg aactccatcc tgagctggcc cggagataag aaggaccctg gcccgccttg
     3481 cagcccaatc cgcaatcccg gcaaaatgac ttggtgacca gttcttggcc gggttgccgc
     3541 ttcccgggtg aatggccaaa atccgttcat tctccttcaa cccagagcgg gctaagtaat
     3601 ccctggaaaa atttaaggcc tccttggaca actggagcgg gtgatccgcg acctcccctt
     3661 cgaacccctt ttccctcagc aattcaatca gataatctga cacatgaacc ttcggtcctt
     3721 ccgaagggaa ggagggcagg ttgagcaccc gggcagcccc ggctttctcc aaattcttgg
     3781 acaaagtctg cccacctttg ccgctgaatg agagaatgag cccgaatgag gagaaaaagc
     3841 tgcacagagg agaaggcagg aaggcgtcgt ccagataaaa ataggccatt ccggcctgct
     3901 ctactgaatg aatggtttcg gccttcaggt cataagccac caaggccagt ctttccgaat
     3961 ggcccattag gtaaagggga aattggcgga gggcatggcg aaagaacctc atggccggga
     4021 gagccaggag caagtctccg attccgccgg agtggatgat gagggttttt cccggattcg
     4081 gtgatgcctt agaaatcata atcgagggcc gagttaagag tcctggggac taggatttct
     4141 ttagatactc cggccatcag catccggaac cccatgggta acttttttgg ccccgaattt
     4201 attctccttc ccggacatca gcctttgaat attctcccta tggcgccaga agatgaataa
     4261 gccgatgaca atggcaaaag gaatgtagat atggtgggga ttgagtaaag caaggaagag
     4321 gggaaatgca gtggcggcgg taagcgaccc cagggaaatg tagcgtgact tgtaaaccac
     4381 tgcggcaaac accaagaatg aaaggaatgc cggaagggtg gccaaggcca ggaaagcccc
     4441 caagccagtg gcaatccctt ttcccccttt aaactttaga aaaatgggat agaggtgccc
     4501 caaaaaagcg gttagggcta cggctgcgat ccagaagtgg gaatcaagca ccgccctggc
     4561 aatgactaca ggaatcagtc ctttcaggat gtcacccacg agggtgagaa cgcccaactt
     4621 ctttcccgcc gtacggtaaa tattggtggc cccgatattc ttgctcccct gggttcgcgg
     4681 gtctacatca ctgaaggccc gggtgagaat gaccccagtg ggcaccgatc ccaggaggta
     4741 ggcaaagata agaagcaata gggtcatggc tcctcccccc caaatgaacg acgatttcac
     4801 caccgcggca tagagaggat agaataaaat ccggaatttc gaatatcgaa attatttgtt
     4861 tcgggaatta agatattgag cctttgtgct ttggctttga atttttaact cagtgttctc
     4921 tgtatttcgg tggtgaaaag gtctttatgc ttttcggacc cggcaaggaa tgtatcggtg
     4981 caacggcgtg ccggcgaagc gatcccggtg cctggccggg gccagccggt tcacgttcac
     5041 cccgtgagcg cggcccccgt gtatgagccc gaagccatgg gggatgacta cctgtccctt
     5101 atgggaagac tcggtaactt ccgcctccac catagccgat ccggcttcgg tctcgatgac
     5161 cgccaggtcc ttatcggcaa tgcccagctt acgggcatct tccgggtgga tgcgcatggt
     5221 gcagtatcgc cggccttcat tccaggccgg gtcccgcata atagaattgg ccaccatctc
     5281 catgtggttt ccggccataa gaatcatagg gtagtctttg ttcaccaggg ccgcgccttc
     5341 ggaagccggg gacacttcct tcacccacga ctccatatcc ggaaagtgaa tgagaacctt
     5401 tttgtccggg gtcttcaggg cagataggcc cttctccgta tccacgatcc ctattttgac
     5461 tccacccggg gtttccagga tcttgccgaa gatttcttct ccagtgaagg gtcccggttt
     5521 gtacccggcc cgggccacat cgtccgggtg catcaggggg aaccgggcca ggagtcccca
     5581 gagggctgac aggttcttcg atcccaattc ctcccccagc gtcttggcca ggatgaaggg
     5641 cagccaggag gccgcttggg ggttggcctg aatgtactgc atgagggcaa tcccgtaggc
     5701 cttccggtcc ggggccagtt ccctcagctt ttgaggatac tcgggaatca tgcccagcct
     5761 ttccgccagt tcgacgtaga cttgggcttc ttccacctgc tctccatcgg gttccaccac
     5821 cgggcggcgc atgtcgaagt agtactccgg atacatccac tggaaaaagg tcccgtccca
     5881 tttttcataa ccgcttcggg ccggcagaac atagtgcgac aggaccgcgg tctcggtcat
     5941 ggccacctcg acagtgacca gcaggtcgag tttggcaaaa gccctttcgt aggctgcggt
     6001 gtcggcgtag gaacgcaagg ggttcgagcc gcttacgatg accgcccgga cccgctgagg
     6061 atggtcatta tctatttctt cgggcataac gttgggcgga aagactccca tgatgagagg
     6121 gatgttggtc gccaccgttc tccaggttcc ggggtcttcc tcgggagtgt gggaccccat
     6181 gggcatcagg tgtccgccga aaacattccc acctttcccc ccgatgcggc cggccagggc
     6241 caggaggatc aattccaaat aggaatttaa tgtgctgtgg cgacccatca gaatgccgag
     6301 gtcgctccgc caggcgctct tacgggtggc gaagaggcgg gtgaattctc gaaccgcccc
     6361 gggatccagg ccgcagaccc ggcagttttc ctcgatattc acatcatcga accaattttg
     6421 aatcgtctca aatccattga ccttagcgga aatgaactcc ctgtccagaa gcccttcctc
     6481 cgtcagcaga attttgatca tggccttcag caggagggca tcggttccgg gtttgatggg
     6541 aagatggata tccgccagtt ttgccgtctc ggtcacccgc ggatcgacca cagccatcat
     6601 gaatccgggc tcttttttct tgttgcggat tactacgggg gcagcaggga tgtcgtgact
     6661 gaccatggga ttggcccccc agaagaccag gaaatctgac ccccggatat cggggacagg
     6721 atgcatgtac tggcggccga aggccttgcc ctgcacccag aaatagccag tgagctcctg
     6781 agccagaggg ctgtagtggt tcttcgaccc cagcccgtgg agaaagcgaa ccccaaaggc
     6841 cgcctcaaaa tggcagccct gccctccgcc tcccatgtag gccacggcgc gcggcccatg
     6901 ccgctccagg acctctttta atttgtccgc gatctcatcc agggcctgct tccaggagat
     6961 tctttcgaat cgatccccca cgcgtttgag gggatatttc aacctctctg ggttgttctg
     7021 aaaataggcg atgtgggcgc ccttccggca gatatacccc tgcgagcgag gattctcctt
     7081 gtccgccctg actctgagga tcttgcttcc ttcggttgtc acttccaggc cgcagttact
     7141 gaaacagaga acgcacgagg tctttttcca ttcgctcatg gccggctcct ttggggattt
     7201 cccctgcagg gagaggatag aacaggataa gggacaaagt caattcggaa tgcaggattt
     7261 tctgttcgga attccgcatt cgcagtcccg tattaagtgt caggctttgg tttcctgctg
     7321 aggcttgact ttcttctgga cgccggaggt atagacgatg atggccgact ccccgtcgac
     7381 cgggcacttg ttttcgcaga ttccacagcc cgtgcatatt tcttccttca cgataggccg
     7441 cttcttatca tcgcccacaa tggcatggta ggaacactgt tcgttgcaga ccaggcagac
     7501 cttcccctct tcataggcga tgcatcgact tttatcgatc ttggccaccc ccatctggac
     7561 cttctgcttt tcttccacag aaagggggcg aatggcttcg gtcgggcaga cccgtccgca
     7621 gaaattgcag tgttcctcac agtagccgat gcgggggacc agtcgggggg tgtagaggcc
     7681 cgacagtcct gcttccagga aagtggattg gagggcgttg ttggggcaga ctttcaggca
     7741 ctttccgcag ccggtgcaga ccgagacgaa tttctgctcc ggcaaggctc ccggggggcg
     7801 aatgagccgg ttgttcttca gcccgctctc ggtttgcagg gggttggttt taatcgctag
     7861 ggcggagaac gcccccaaaa ccagcgaccc cactacatac ctgcgggaga gagaaaccgc
     7921 ttccgcgccc ttccacctgg gaaagtggaa accgaagacc gtggcctccg gcgggcaatc
     7981 caggcacttg aagcaagaga tgcaatcctg ctggcgatac tcccggggag tttcggcggg
     8041 gatggcacct acggagcact ctttggcgca cttcaggcac tggatgcagg cctcgctgac
     8101 cgaccgcctt acgacccgga ggcgagaagt gagcgaaaag agcgtcccca gggggcagag
     8161 gaagcggcac cagaacctct tgcgaatgac tcccaatgcc agaactcccg tgaagaagag
     8221 aaagatcagc aagttgactt tgaagacggg cagtttcatt gccggcagga tgtaaggatt
     8281 gctgggaaga aaccgctgga cctccggaag aagatgatta tagatgtaaa ccgcgggcgg
     8341 gtaaaaggcc acgaccagag tccgggtaat caggcttatg gggtcgagta ggtatagaac
     8401 ctggagtccc atgatccccg cggccacggc gaagatgaaa gccccgtagc ggaagcgcct
     8461 caggggctga tcgtcaaaag gcttggagcg cttcttctcc cggaagagga cccggtcgaa
     8521 aaaatcgagg gtcgctccca tggggcagaa ccatccgcag aagaaatttc cgaagacgat
     8581 taccaggagg aggatgccga aggccggcag catgcgcatg atcacctccc cttgcgtgag
     8641 aaaggtgatg atggcgatga aagggtccag ccggagatag aggtcgactg gaatccttat
     8701 ttccaaaggg aaggcagtct gaatgattaa ataaagaagg aataaaaagg agatgacctg
     8761 gacggttctc ctcagatact ggagctttcc ggtcatgccg ccttgacctt cttgatcttg
     8821 atcttggaca ggttcatctc ccccaggccc aagtcgtacg cgttcttgat gaactggata
     8881 tcggccggcc ggaagggctt tcccgtcttg ggatgcttaa aataggtcgc gcaatgagca
     8941 tccacggcca ccatatccgt ggcagccatc acttccccta tgtcctcggt cttgcccggc
     9001 cctttgggtc cgttggtagt gaggatccgg atggcgtcga ccacaaccag gtccggcttg
     9061 cggatggtgt tgatgtcggc aatgcactgg tgaaggtcga tcttgtgcag aagttccatg
     9121 tcccaggtga cccccatggt gttcttgagg gcgagggtga gttcggtggc gaaatgctgc
     9181 ttgggaaggg ggaaattgat gagcacatcc gtctccagga aatcctgtat aaactcggct
     9241 tccttgagct tcttgccctt gggaatctcc aacttcttat atacattccg tccgtggccg
     9301 gaataaaggt ctcccccggc atcctgagtg gccttcttca aacccgaccg ggagaagcaa
     9361 aactgataat tgtcacaggt atgatcccag accgccaccc gcttggcccc tgcctggtaa
     9421 cacatcttga tcaaagcgga gagcagctcg ggattgttgg tggcagcctg ttcgggcgtg
     9481 cgggcccagg cgatgttggc cttgatcacc actctttgtc ccttttttac aaaacgcccc
     9541 atgcctccca gcggggccat ggcggcctgc agaagctgtg tcggagtccc ctgctgggca
     9601 ataatcaggt cggcgtcgcc cgcggcatag gccacggagg ggcgcaacgg ggaagagagg
     9661 aagaacatct tgggcagggc aaccgctgcg gccgcccctt tcataaaatc ccggcgggtc
     9721 aaattcaatc tggaggacca atccataact gccctttctt tccgtattgc ttttcagctt
     9781 cggcttattc catatcatga ttgcccagga cgaaagaatg atttagaaca gccagaactt
     9841 tatttcgaaa tcgggttttt ggtctcggaa tcccaacttc ccctttaaaa tcgatctacc
     9901 ttctttccca ccttgccttc cgcattcgga gtttcacgtt tcccattcac tctggctgtt
     9961 tcatcaggga ggcaccctgg tcggtcccga accatacccc gcctttgtct tctgtcgcgc
    10021 agaacacatt gttgctgggc aaaccatcgg cggtcgtgag cgaatcccac tggttctggg
    10081 aaatcccata aacgctgacc ccattatcac ggcagacaaa aaccttcccc tgaatggcgg
    10141 ccaggcagta aattcctccg ttcttcaggg aatcggtggg ttcaattctt ttccaccctt
    10201 ctgcaggggg atcatattca tagagggccc cctggctgct tccggcccaa atcttatccc
    10261 caatctgggc tagggctttt atctccaagt ccgtcaagcc ttcctgctgg ccgtaattct
    10321 tccatttcat ctggcttttt tgcaggcggg tcacccctcc attggttccc gcccacaaaa
    10381 agtcgccctg aaccagaagg gcattaacga aattcccgga aaggccatcc ttggaggagt
    10441 aaaaaacctt ccatttttcg gtctttttgt ccaaggccga aatgcctctc tccgatccaa
    10501 cccaaagaat gtcgccgtcc atggccaaag aaacgatctt cttggctagc ccgttgttgg
    10561 tgttgaaggg tctccagggg ttcttcctct tgggttggta ataagagatc cctccccctc
    10621 cgaacccgta aaagtaagtg ccgaaccaga gccggtcttt atccggaaga ataaccgtga
    10681 cgtcgttgta attcgggtgg tttttccact tgatggccac cccgtcatct ttttccggag
    10741 gttctccctt ggtggtaaag acctggaagg tattcttgga ccgattgtat agagtcgttc
    10801 ctccggcata ggtgccgaac cagacttcat ctccctgcac ggcaatggcg aagatcatgt
    10861 cgctggacaa cccttccttg acggtgagct gctgaaagga aaccggtctt ttgatgaaat
    10921 cagcttctgc gggaaaacca gggcttaaaa gtagaaaagt cgaaaggata atcattccaa
    10981 atacgttggc agcttgcctc attcaccctc cttatccaaa tattatcgcg gattgaaggc
    11041 tgtttttcaa taaagaaatc acctgattag gctgattcgg cgtgccaatc aactcagaaa
    11101 aatgttttta ctggggattg gacattctat agcggtcggg aaatgggtcc gaagaaaaat
    11161 tcccaagcca aaaatttttg gtataataaa aatttttggg ggagcggacc cccttttccc
    11221 gggtggtttt tcaagaatct tccctctgaa ggaaagggca gggtatgagt ggaatttttg
    11281 ggatcgtatc gaataacaat tgtgcttcca ccctttttta cggcaccgat taccattctc
    11341 acatgggaac cgagtacggc ggcatggccg tactgggaac ccggtttaat cgggccatcc
    11401 atgacattag ccaatcccag ttcaaatcca aattcgtaga cgaatacaaa accatgcagg
    11461 ggcccatggg aatcggtgtg atcagcgacc gtgaccccca gcccctcatc gtaggctcca
    11521 ggttcggaac cttcgccatt gtcaacgccg gcctgatcga gaatctggag tccttgagca
    11581 ccgagctctt ggagcaagga cattccttcg gagaaatgac cagcgggggg atcaactcgg
    11641 ttgaactggt ggccaaactg atcaacaccg gcaaggattt ggtcgatggc ttcgatagga
    11701 tgttggacaa aatccatggg gccgcaactc tccttctttt gaccagggaa
//
LOCUS       DASPRR010000452         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_426435, whole genome shotgun sequence.
ACCESSION   DASPRR010000452 DASPRR010000000
VERSION     DASPRR010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426435"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VEL68_16590"
     CDS             <1..1012
                     /locus_tag="VEL68_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXZ37642.1"
                     /translation="RKTPMKTMFVNSGAEAVENGVKIAKYYTKRPGVICFENAFHGRK
                     AMTMSLTSKVKNYKFGYWPFMPEVHRIPYAYCYRCSYGLKYPSCKVYCADFIQEKFFD
                     LYAAAESIGAIIVEPVQGEGGFIVPPKEYLGKLQKTCKDNGIVLIVDEVQTGFARTGK
                     MFAYEYSIDEPDILITAKSLAAGLPLGGITGKAEIMDSVDAGGIGGTFGGNPVCCAAG
                     LAVLKILKEEKLADRSNEIGKKVMAKFRSFQKKYPFVGDVRGLGAMNGMEIVADKKNP
                     KPNADLTKSIVKRCYDKGLIILTAGGYGNVIRHLVPLVITDEQLDRGLKILDEAMAEK
                     VE"
     gene            1131..2393
                     /locus_tag="VEL68_16595"
     CDS             1131..2393
                     /locus_tag="VEL68_16595"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37643.1"
                     /translation="MSRRCRLVRFLLLFVTLALFASGPVLAAAPPKAIKIGITAPLTG
                     PAAEAGVALKQGFILAMEEWNANGGVMLKEFNKKVPLEVLVEDCQSKPEVGVAIAEKL
                     FTRDKVDVLLGDAFHSSVTMAIMELAPKYGKPIMSVEPVSEEIAKKIASNPKRYWNFW
                     KGDWGSTGYADSVFSTYRFMESKGLFKSKTNKIAFIVEDTDYGRSNADKTKELFAGIG
                     FQSVAMETVPLGYTDFYPQLGKLKNLGPDVLMSVFTPLSSGVALTKQFQEVGLKAYHH
                     AIYYPLRPEFIPQAGKSADFLLWSTLLVDPENNPKHKEFGEKIKKRWNVVVNSDHASA
                     YDGLHNILDSIQRAGSLDPKKIVDALSKLDLKGIMGRYVFEQSNHQIKYGEDFVPVPA
                     CQIQEGKHRVIWPASLAAGTFQLPPWLK"
     gene            2669..>2872
                     /locus_tag="VEL68_16600"
     CDS             2669..>2872
                     /locus_tag="VEL68_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ37644.1"
                     /translation="MEQKKNGLLLEGQGVTKYFGGLAAINDVSFFLNQGEILGLIGPN
                     GAGKTTLFNIIAGVYKPNQGKVFF"
BASE COUNT          722 a          739 c          811 g          600 t
ORIGIN      
        1 caggaagact cccatgaaaa ccatgttcgt caacagcgga gccgaggcgg tggagaacgg
       61 ggtcaagatc gccaagtatt acaccaaaag gccgggggtc atctgcttcg agaacgcctt
      121 ccacgggcga aaggccatga ccatgagcct aaccagcaag gtaaaaaact acaagttcgg
      181 gtactggccc ttcatgcccg aagtgcaccg gattccttac gcctactgtt accgttgttc
      241 ctacggcctg aagtatccct cctgcaaggt ctattgcgcc gacttcatcc aggagaagtt
      301 cttcgatctt tacgcggcgg cggaatccat cggggcgatt atcgtggagc cggttcaggg
      361 agaagggggt ttcatcgtcc ctcccaagga gtacctggga aagctgcaga agacctgcaa
      421 ggacaacgga atcgttctga tcgtagatga ggttcaaacc gggttcgccc ggacgggaaa
      481 gatgttcgcc tatgagtatt ccatcgacga accagacatc ctgattaccg ccaaatcctt
      541 ggctgcaggt ctccccctgg gcgggatcac ggggaaggcg gagatcatgg attcggtgga
      601 cgccgggggc atcggcggca ccttcggggg caacccggtg tgctgcgcgg cggggctggc
      661 ggtactgaaa atcctgaagg aagaaaagct ggcggaccgg tccaatgaaa tcgggaagaa
      721 ggtcatggcc aaattccgct ctttccagaa aaaatacccc ttcgtcgggg acgtgcgggg
      781 cctgggggcc atgaatggca tggaaatcgt ggccgacaag aagaatccca agcccaacgc
      841 ggacctgact aagtccatcg tcaagcgatg ctacgacaag ggcctcatca ttctcaccgc
      901 cggaggttac ggcaacgtga tccgccactt ggtgcccctg gtgatcaccg atgagcagct
      961 cgaccggggc ctcaaaattc tcgacgaagc catggcggaa aaggtggaat gaacgaagaa
     1021 aatcggaaag agcataaggc agagcgtata tagggcaatc tttgtaggga tagattcagc
     1081 ggcaggtttg gtcaagcctg gttgataaaa agaaaaaagg agggttcgcc atgagcagaa
     1141 gatgcaggtt ggtccggttt ttgttgcttt tcgtgaccct ggcccttttc gcttcggggc
     1201 cggttttggc cgcagcaccg cccaaagcca ttaaaatcgg cattaccgcc cctctgaccg
     1261 gcccggcggc agaggcgggg gtagccctta agcagggatt catcctggcc atggaagaat
     1321 ggaacgccaa cggcggggtc atgctcaagg agttcaacaa gaaggtccct ttggaagttc
     1381 tcgtcgaaga ttgccagtcg aaaccagaag tgggggtggc cattgccgaa aaattgttca
     1441 cccgcgataa agtggacgtc cttttggggg atgccttcca cagctcggtg accatggcca
     1501 tcatggaact ggcgcccaaa tacggaaagc cgatcatgag cgtggagccg gtcagcgagg
     1561 aaattgccaa gaagatcgcc agtaacccca agcgatactg gaacttctgg aagggagact
     1621 ggggcagcac gggctatgcc gactcggtgt tttccacgta ccggtttatg gaaagcaaag
     1681 ggcttttcaa atccaagacc aacaagatcg cctttatcgt tgaagacacg gattatggaa
     1741 ggtccaacgc cgataaaacc aaagaattat tcgcggggat cgggtttcag agcgtggcca
     1801 tggaaaccgt tcccctggga tatacggatt tttaccccca actcggaaaa ctaaaaaatc
     1861 tcgggccgga tgtattgatg tccgtcttca ctcccctttc ttccggcgta gccctgacca
     1921 aacagtttca agaggtgggc ttgaaagctt atcaccatgc gatttattac cccttgcgtc
     1981 cggagttcat cccccaggcc gggaaatccg cggacttcct cctctggtcc acgctgctgg
     2041 tggacccgga gaataatccc aagcacaaag aatttggcga gaaaatcaag aaaaggtgga
     2101 atgtggtcgt aaacagcgac cacgcttcgg cttatgacgg gctgcacaac attttggatt
     2161 cgatccagcg ggccgggtct ctggacccca agaagatcgt ggacgccctt tccaagctgg
     2221 acctgaaagg aatcatgggg cggtacgtct tcgagcagtc caaccaccag atcaaatatg
     2281 gcgaagattt cgttccggtc ccggcgtgcc aaatccaaga ggggaagcac cgtgtcatct
     2341 ggcctgcgag cctggccgcc ggcaccttcc agcttcctcc gtggctgaaa taaagaaact
     2401 ttcggggacg cagatttcgc ggattaataa aagaacctaa aatctaagaa ccatcagatg
     2461 cattttttaa gcatgacgac atgaatttca tcgcgggggt cattccgggc ttaacccgga
     2521 atccagtttt tttctggatt cccgctttcg cggaatgaca cctcttcctg gagttcaagt
     2581 cgctgtgaat tgaattggct ttaggtttga atttcctttt gggcttatct gcgtaagtct
     2641 gcgagatctg cgtcccaatg aaaaaaacat ggaacagaaa aagaacggtc tcctgctcga
     2701 agggcagggg gtgaccaaat acttcggcgg gctggccgcg atcaatgacg tctctttttt
     2761 tctgaaccag ggagaaatcc tgggcctgat cggaccgaac ggagccggca aaacaaccct
     2821 ctttaacatc atcgccggag tctacaaacc caatcaggga aaggtctttt tc
//
LOCUS       DASPRR010000453         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2359320, whole genome shotgun sequence.
ACCESSION   DASPRR010000453 DASPRR010000000
VERSION     DASPRR010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359320"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VEL68_16605"
     CDS             <1..316
                     /locus_tag="VEL68_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37645.1"
                     /translation="LMASSKILLLDEPSLGLAPVVVQEVFRVIKEINKMGTTILLVEQ
                     NARMALLLASRGFVMETGKVMLADSAANLLANEEMKMHYLGVHTHVKEARQRRRGKGL
                     PK"
     gene            complement(586..2115)
                     /locus_tag="VEL68_16610"
     CDS             complement(586..2115)
                     /locus_tag="VEL68_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXZ37646.1"
                     /translation="MLAKVLSGAILGIDAYMVEVEVDIAQGLPVFATVGLPDGAVKES
                     KERVKSAIKNSGYDFPPKRITVNLAPADVKKEGAGFDLPMAVGILSALEVISPPLLDE
                     YLLLGELSLDGRVKPIRGALSLAMSAKEKGKKGVLLPKENAEEAAVVQGIEVLAIEVL
                     SEAVDFLNGLKPIPPTGVDLQEIFRREKHYSDDFNEVKGQEHVKRALEIAAAGGHNLI
                     MIGPPGAGKTMLAKRIPTILPDLSFEESIEITRIYSVMGLLPPHSSLIAVRPFRSPHH
                     TISDAGLIGGGQIPKPGEVSLSHNGVLFLDELPEFKKNVLEVLRQPLEEGRVTISRAL
                     SSLTFPSSFMLIAAMNPCPCGFYTDPNRECTCTMPQILRYRSKISGPLMDRIDIHVEV
                     PAVKYRDLTGENSGEDSETIKARVNKAREVQRERFASKKIYSNAQMTSRHLKKYCQIG
                     ENSKALLEQAIDKLGLSARAYTRILKIARTIADLEGVQDIATHHISEAIQYRSLDRTL
                     L"
     gene            2233..2715
                     /gene="moaC"
                     /locus_tag="VEL68_16615"
     CDS             2233..2715
                     /gene="moaC"
                     /locus_tag="VEL68_16615"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXZ37647.1"
                     /translation="MGKLTHFDKAGRARMVDVGTKIPTRRIAVASGKVIMKPGTLRRI
                     LDKKIEKGDVLGVARVAGIMAAKRTGEIIPLCHPLSIDTAEINFQPDVKQSEVKIETT
                     VKSTGKTGVEMEALLATAAAALTIYDMCKAVDRGMKVGEIMLLKKSGGKSGTYIRRTK
                     "
     gene            2845..3219
                     /gene="dksA"
                     /locus_tag="VEL68_16620"
     CDS             2845..3219
                     /gene="dksA"
                     /locus_tag="VEL68_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HXZ37648.1"
                     /translation="MEKERLEYFRELLNERMKTLLEEAEKTVTGMTNERDTFPDPTDR
                     ANLETDRNFLLRIRDRERKLIGKIKEALARIDDGTFGICEECGDDISEERLKARPVTT
                     LCIDCKTKAEEEEKEKKKASLA"
     gene            3234..>3796
                     /locus_tag="VEL68_16625"
     CDS             3234..>3796
                     /locus_tag="VEL68_16625"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ37649.1"
                     /translation="MKVKWYGHAAFLLTSEQGTKIITDPYEPGAFGGAIAYGPIPDQA
                     DIVLVSHDHADHNYVQGLKGKPQIVKGTGSHKVNNLEFKGIAAYHDGRKGSERGQNTI
                     FCFSLDGLSVCHLGDLGHVLSDAEAKQIGPVDLLLMPVGGVYTIDPNQASQTAQKLSP
                     KIVIPMHYKTPHCGFPLAKAEDFTSGKA"
BASE COUNT          940 a          953 c         1129 g          774 t
ORIGIN      
        1 tctgatggcc agctcgaaga ttctgctttt ggacgagccc tctctggggc tggcgccggt
       61 cgtggtgcag gaggtgttca gggtcatcaa ggagatcaac aagatgggga cgaccatctt
      121 gctggtggag cagaacgccc gcatggctct cttattggcc agccgcgggt tcgtaatgga
      181 gacgggcaaa gtcatgttgg ccgatagcgc ggcgaatctc ctggccaacg aggagatgaa
      241 aatgcactac ctgggcgtcc acacccatgt caaggaagcg cgccaacgcc gacgtggtaa
      301 gggattgcct aaatagtgga aggcttcgaa agtggctagg gccctgccga atgagacagc
      361 aggccctttt tttgtgcaaa acgcgtcgct caacagttct gatgtcaccc atcagccccc
      421 tgaaccaata tggtacatgc ccaatgattg agattaggca gttttgataa ttttaatagc
      481 ttcgattctt atgcaggagt tgccggacct tccccatata ttggtcaata gtctcaatgc
      541 cggtcttagt acccaatatg ggcccaggga ttgataacgg ggttttcata acagcgttcg
      601 gtccaggctg cggtattgga tggcctcgga gatatggtgg gtggcgatgt cttggacacc
      661 ctcgaggtca gcaatggtgc gagcgatttt gaggatgcga gtgtaggctc gcgcggagag
      721 gcccagcttg tcgatggcct gctcgaggag agctttggag ttttccccga tctggcagta
      781 tttcttcagg tgccgggagg tcatctgggc gttggagtag atttttttgc tggcaaagcg
      841 ttcccgctgg acctcccggg ccttgttcac ccgggctttg atggtttccg aatcctcgcc
      901 tgaattctcc ccggtcaggt cccggtattt gactgccggg acttccacat ggatgtcgat
      961 ccggtccatc agggggcctg atatcttgga tcgatagcga aggatctggg gcatggtgca
     1021 agtgcattcc cggttggggt cggtataaaa cccgcagggg caaggattca tcgcggcgat
     1081 cagcataaaa gaggagggga aggtgagtga agatagggcc cgggagatgg tgacccttcc
     1141 ttcttccaag ggctggcgca gcacctccag gacgttcttt ttgaactcgg gaagctcgtc
     1201 caggaagaga accccgttgt gggagaggct gacctcgccg ggcttgggga tctgccctcc
     1261 tccaatgagg ccggcatccg agatcgtgtg atgaggagag cggaagggtc ggacggcgat
     1321 aagcgaactg tggggcggaa gaaggcccat gacgctgtag atacgggtga tctcgatgga
     1381 ctcctcaaag gagaggtccg ggaggatcgt gggaatccgt tttgcaagca tcgttttccc
     1441 tgcaccggga gggccgatca taatgaggtt gtggcctccg gccgcggcga tctccagcgc
     1501 ccgcttcacg tgttcctggc ctttgacctc gttgaaatca tccgaataat gtttttcccg
     1561 tcggaaaatc tcttgcagat caactccagt tgggggaata ggctttaacc cattcagaaa
     1621 atccacggct tcggagagaa cctcgatggc caggacttca attccctgaa cgaccgccgc
     1681 ctcttcggcg ttctccttgg ggagaaggac gccttttttc cccttttcct tggccgacat
     1741 ggccagggag agggcaccgc ggatgggttt aacccggccg tcgagggata gctcgcccag
     1801 gaggagatat tcatccaaaa gcggcgggga gataacttca agggcggaaa gaatgcccac
     1861 cgccatgggc aggtcgaacc cggccccttc cttcttaaca tcggcgggag cgagattcac
     1921 cgtgatacgc ttgggaggaa agtcatagcc ggaattcttg atggccgact tgacccgttc
     1981 tttggattct ttgaccgctc catcgggaag tcccacagtg gcgaaaaccg gaaggccctg
     2041 ggcaatatcg acttccactt caaccatata ggcatcaatc cctaaaatcg caccgcttag
     2101 gactttggcc agcatccggg tcctctctca aattcgacga gaagggcact aaagcgttga
     2161 agaggcggca aaaattccct atacttatag caaaaagggt tcgggcaaag caagggagaa
     2221 aagggggtgg ggatgggaaa gctgacccat ttcgacaagg cggggcgggc gaggatggtg
     2281 gatgtgggga cgaagattcc cacccggcga attgcggtag cttccggcaa ggtaatcatg
     2341 aagccaggga ccctgagaag gatcctggat aagaaaattg aaaaggggga cgtcttgggt
     2401 gtagcccggg tggctggaat catggccgcc aagcgcaccg gcgagatcat cccgctttgt
     2461 caccctctga gcatcgacac cgcggagatt aacttccaac cggatgtgaa gcagtcagaa
     2521 gtaaagattg aaactacggt gaagtccacc gggaaaaccg gcgtcgagat ggaggccctc
     2581 ttggccacgg cggctgcggc ccttaccatc tatgacatgt gcaaagccgt agaccggggg
     2641 atgaaggtgg gggagatcat gctgctgaag aaatccgggg ggaagagcgg aacgtacatc
     2701 cgaagaacta agtaaaatgg gaaatgggga attcggaggt ccaagaaaaa aagggggggg
     2761 ttcataagag aaagaaaata gttgacccct ggtaaaatgt gatagaatag caacttgtta
     2821 gggtgatagc ttgagggaaa gcagatggag aaggagaggc tcgaatactt ccgggagctc
     2881 ttgaacgaac gtatgaaaac cctcctggaa gaagcagaga aaacggtcac tggaatgacc
     2941 aatgaaaggg ataccttccc cgacccaacc gaccgggcca acctggaaac cgaccgcaat
     3001 tttctcctcc gcatccgcga ccgagaaaga aagctcatcg gaaagatcaa agaagcattg
     3061 gctaggatcg acgacggaac gttcggaatc tgcgaggaat gcggcgatga catctcggaa
     3121 gaacgcctca aggcccgtcc cgtaaccacc ctgtgcatcg actgcaagac aaaagcagaa
     3181 gaagaagaga aagaaaagaa gaaagcctcc ctcgcttaga aaggaacccc ctgatgaaag
     3241 tgaaatggta tgggcatgca gctttcctgc tcacttcaga acaagggacc aagatcatca
     3301 ccgaccccta cgagccggga gcattcggcg gggctatcgc ctacggcccg atccccgatc
     3361 aggccgatat cgtgctggta agccatgatc atgccgatca taattatgtg cagggtctca
     3421 aaggaaaacc ccaaatcgtt aaaggaacgg gctcccacaa agtaaataat ctggaattta
     3481 aagggatcgc tgcctaccac gacggacgca aaggctccga aaggggccaa aataccattt
     3541 tctgtttttc cttggatgga ctaagcgtct gccacctggg cgacctcggt cacgtgcttt
     3601 ccgatgcgga ggccaaacag atcggtccgg tagacctcct ccttatgccg gttggcggag
     3661 tatacaccat cgaccccaac caagccagcc agactgctca gaagttaagc cccaagattg
     3721 tcatccccat gcattacaag acccctcact gcggatttcc tttggccaaa gcggaggatt
     3781 tcacctcagg caaggc
//
LOCUS       DASPRR010000454         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1881544, whole genome shotgun sequence.
ACCESSION   DASPRR010000454 DASPRR010000000
VERSION     DASPRR010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881544"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..618
                     /locus_tag="VEL68_16630"
     CDS             175..618
                     /locus_tag="VEL68_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37650.1"
                     /translation="MRGPLFYRSLFNFRISELNPIRVICAVYILGFGYGTRNHIVDIF
                     ADGWLGYDYVPLPINLYWTLLTFFDPLAILLLLSFPFAGILLSVLIMASDIAINIGVA
                     VYFYYQTGMLTLDRLPLQIAFGTFVFLTSPIAWKTVKRSSRDRSA"
     gene            642..1112
                     /locus_tag="VEL68_16635"
     CDS             642..1112
                     /locus_tag="VEL68_16635"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HXZ37651.1"
                     /translation="MLKEVQAYLTKLSDLRNQAKSLLDGLPAEALDWRPIRGEGEMAT
                     NSLLVIAAHLAGSQTYWMKEIIGLTPIQRDREAEFATKGVSTADLKAKLDTAGKIAEE
                     ILSPLTESQMEEGRKFRDKPITVRGGILQVIDHMAQHIGHMQLTRQLWMAKSPK"
     gene            1178..2167
                     /locus_tag="VEL68_16640"
     CDS             1178..2167
                     /locus_tag="VEL68_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXZ37652.1"
                     /translation="MLPITLKTILDLSRNFMESRILLTGAELNLFTLLASSPLTAQEV
                     AARIGANLRALIIVLDALAAMELLAKKEEKYSCPPEISFLLSEKSPESVLPMVQHMAH
                     VWQRWSKLTEKVKGREEPEKPTESQEADQIPAFIGAMHAIAARLAPGIVAAVSPVTAR
                     NLLDVGGASGTYTLAFLRAVPQMRATLFDKPEVISIARKRLAEAGILDRVTLVGGDFY
                     LDEFPPGHDLVFISAIIHQNTPDQNLGLYQKGFRALLPGGRIVIRDHVMEPDRTQPRD
                     GAIFAVNMLVGTPGGNTYTYEEIHAGLEKAGFTRIRMLQKGEKMDGLVEGFKP"
     gene            2223..2435
                     /locus_tag="VEL68_16645"
     CDS             2223..2435
                     /locus_tag="VEL68_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37653.1"
                     /translation="MAERKNIKVKTFATELKEFKAMRELADLDGQVNKFIKEKKVKKV
                     VSVSDATTTDTTGASIGLIRVLVYES"
     gene            complement(2446..2979)
                     /locus_tag="VEL68_16650"
     CDS             complement(2446..2979)
                     /locus_tag="VEL68_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HXZ37654.1"
                     /translation="MRKIFAALMLATAILFSCVPLSKEVMRQVDETLTYQTVQKNPQD
                     FLGKTVLWGGVIVKTTNRQDETILEVRQSELDLEKRPKSPDSSAGRFIVRQAGFLDPV
                     IYDAGREITVAGEVAGREILPLAGIQYTYPVILAKEIYLWEKIDYPYPYWDYPPYWGP
                     YWYPYGYPYWRNRPYWW"
     gene            complement(2976..3467)
                     /locus_tag="VEL68_16655"
     CDS             complement(2976..3467)
                     /locus_tag="VEL68_16655"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HXZ37655.1"
                     /translation="MRLISIIVLICFLTACTQAIPEESLKTADPDLTFQKLIREPEQY
                     RGRIILTGGQILGTTAREGETWVEVLQKPLDWQHKPKDTDESFGRFLIRFESFADPAI
                     YAAGKKITVLGEVLGKKVQPLKEMDYAYPVLTPREHYLWKPEDSSSPSFHFGIGVGVG
                     VYR"
     gene            3673..4239
                     /locus_tag="VEL68_16660"
     CDS             3673..4239
                     /locus_tag="VEL68_16660"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXZ37656.1"
                     /translation="MADFFELLKTRRSIRDFQEKEVPIELIQAIIKDTCMAPSSGNGQ
                     PWKFIVVNNRNKIQKLSDESKKNLLFSIEKDPASPLKKYEAALRNKDFNVFYNAPCLV
                     YIVGEKEVRSLWVDCTLAACYFMFSAAARGLGTCWVDLGSQVRSPEILKEIGLPETCQ
                     IVAPMALGYPKSIPSPSERKAEILKIIS"
BASE COUNT         1056 a         1227 c         1148 g         1054 t
ORIGIN      
        1 aaagctatta tcgccaatat ggcctgaagg attgggccaa agaacaatag agggttcttg
       61 ccttaaatgc acaagcctgt tcggattgca gggcctgttt ggagcgatgc ccatacggag
      121 tttccatccc ctctggttta aagaaggccc ataacatcct tcagagccca taaaatgcgg
      181 ggccctttat tttacaggtc gttatttaat ttccgaatca gcgagttaaa tcccatccgg
      241 gtcatctgtg ccgtctacat cttgggtttt ggttacggga cccggaacca catcgtcgat
      301 atcttcgccg atgggtggct gggatacgat tatgtccctc tccccatcaa cctttactgg
      361 acgcttttga cttttttcga tcccctggcg atcttgcttc tcctctcctt tccttttgcc
      421 ggaatccttc tttcagtttt gatcatggcc tcggatattg ccatcaacat cggggttgct
      481 gtatatttct attaccagac gggcatgcta accctggatc ggttgccttt gcagatcgcc
      541 tttggaacgt ttgttttcct cacttcaccc atagcttgga agacggttaa aaggtcgagt
      601 cgggatagat cagcataatc ttgcccaagg aggaatggaa aatgcttaaa gaggtccaag
      661 cctatctaac gaaactcagc gacttgcgaa atcaggccaa atccctgctg gatggattgc
      721 cggcggaagc cctggactgg aggcccatcc ggggagaagg cgagatggcg accaattcac
      781 tgttggtcat tgccgcacac ctggccggat cgcagaccta ttggatgaag gaaatcatcg
      841 gcctcactcc cattcagcgg gaccgggagg cggaattcgc cacgaaagga gtcagcacag
      901 ccgatttgaa ggccaagctg gataccgccg gaaaaatcgc cgaagaaatt ttatcccccc
      961 tgaccgaaag ccagatggag gaaggacgga agtttcggga caagccgatc actgtccggg
     1021 gaggaatcct acaggtcatc gaccacatgg cccagcatat cgggcatatg cagctgaccc
     1081 ggcagctctg gatggcaaag tcgcccaagt agccaatttg ataaactgtt tgggggacaa
     1141 ttgaagaatt gcccccacct tgaaaagcga aggattcatg cttcccataa ccttgaaaac
     1201 catcctggac ttgtcccgga atttcatgga gagccggatt ctgctcaccg gagccgagct
     1261 caatcttttt accctcctgg cctccagtcc tctcaccgca caggaggttg ccgcccgaat
     1321 cggagcgaac ctccgggcgc tgatcatcgt cttggacgcc ctggcggcga tggaactcct
     1381 cgcgaaaaag gaagaaaagt actcctgccc tcccgaaatt tcttttctgc tctcggaaaa
     1441 aagccccgaa tcggttctgc ccatggtcca gcacatggcc catgtctggc agaggtggtc
     1501 gaaactgacc gaaaaggtca aaggacgaga agagccggag aaacccacag aatcccaaga
     1561 ggcagatcaa atccctgctt tcattggggc catgcacgcg atcgcggcca ggctggcccc
     1621 ggggattgtt gccgcagtca gcccagtgac ggcgaggaat ttgttggatg tgggcggggc
     1681 ctcggggacc tataccctgg cctttctccg ggccgtcccg cagatgagag ccaccctttt
     1741 cgataaaccc gaagtcatct ccatcgcccg gaagcgcttg gctgaagcag ggatcctgga
     1801 tcgggttact ttggtgggag gggattttta ccttgacgaa tttcctccag gccacgacct
     1861 cgtctttatt tcagccatca tccaccagaa tacccccgac caaaacctgg gcctttacca
     1921 aaaaggattc cgggctctac tgccgggagg aagaattgtt atccgggacc acgtgatgga
     1981 accagaccgg acccagccca gggatggagc catattcgcg gtaaacatgc ttgttgggac
     2041 tcccggggga aacacctata cctacgagga aattcatgcc ggtctggaaa aggcgggttt
     2101 cactcgcatc cgtatgctgc agaaagggga gaaaatggat gggctggttg aggggttcaa
     2161 gccgtagggg cgatccggcg ggttgccctt acacaaaccg ccaaaaagtg gaggaaaaga
     2221 acatggccga aagaaaaaac attaaggtaa agaccttcgc caccgaattg aaggaattca
     2281 aagccatgcg ggagctggcc gacctggatg ggcaggtcaa taagttcatc aaggagaaaa
     2341 aggtcaagaa ggtcgtatcg gtcagcgacg ccaccaccac cgataccact ggcgcctcga
     2401 tcgggttgat ccgagttttg gtttacgaga gttgacgccc aggctttacc accagtaggg
     2461 ccggtttctc cagtaggggt acccgtaggg ataccagtat ggcccccaat atggaggata
     2521 gtcccagtac gggtagggat agtctatttt ttcccagagg taaatttctt tggccagaat
     2581 aaccgggtag gtgtactgga tacccgcgag gggtagaatt tctctgcccg caacctcacc
     2641 tgcaacggtt atttcccgcc ctgcatcgta aatgaccggg tcgagaaatc cggcttggcg
     2701 aacgataaac cgtccggcgg aagagtccgg gctcttgggc cttttttcta aatccagctc
     2761 agactgtcgc acctctagaa tcgtttcatc ctgtctgttc gtagtcttta caatcactcc
     2821 gccccacaaa acggtcttgc ccagaaaatc ctgggggttt ttctggacag tctgataggt
     2881 cagggtctcg tccacttgcc gcatgacttc cttggaaagg ggtacgcagg aaaacaggat
     2941 cgcggtcgct agcatgagtg ccgcgaaaat ctttctcatc tgtatacccc cactcccacc
     3001 ccgatcccaa aatgaaaact gggactgctt gaatcttctg gcttccacag gtagtgctcc
     3061 cgaggagtca gcacgggata ggcataatcc atctctttca ggggctggac tttcttcccc
     3121 agcacttctc ccagaaccgt aatttttttc cccgcggcgt agatcgccgg atcggcaaag
     3181 ctttcaaacc ggatcaaaaa tcgcccgaaa gattcatccg tatctttggg cttgtgctgc
     3241 cagtccaggg gtttttgaag gacctcgacc caggtttctc cctcccgagc agttgttccc
     3301 aggatctgcc cgcccgttag gattattctc cccctgtatt gttcgggctc cctgatcagc
     3361 ttttgaaaag tcagatcggg gtcagccgtc tttagagatt cctccgggat cgcctgcgta
     3421 caagcggtca agaagcatat caggacgatg atactgatca gcctcatcta acttcgctcc
     3481 ttactccatg atttctcggg gggaaatggg ttcctttcta tccattttag tttattatac
     3541 caggtattta ggcctttgga aaaagtcagc tgcctccctt aagcctttcc cttccccgcc
     3601 attgtcggat aaaatgatag caacgatgga gggggtccgt gaatcgtccc cgccaaggaa
     3661 aggagccaaa agatggccga ctttttcgag cttttgaaga ctcggcgttc catccgggat
     3721 tttcaggaaa aggaagttcc gatcgaactg atccaggcga ttatcaagga cacctgcatg
     3781 gcccccagct cgggcaacgg gcagccctgg aagttcatcg tggtcaacaa ccggaataag
     3841 atccagaagc tttccgatga aagcaagaag aatcttctct tctctattga aaaggacccg
     3901 gcctctcccc tgaagaaata cgaagccgcc ctgcgcaaca aggacttcaa tgttttctac
     3961 aacgccccct gcctagtgta cattgtcggg gaaaaagagg ttcgttccct ctgggtcgat
     4021 tgtaccctgg ccgcctgcta ttttatgttt tcggcggcag ccagggggtt gggaacctgc
     4081 tgggtggacc tgggctcgca ggtcagaagc ccggaaatct tgaaggaaat cggcttgcca
     4141 gaaacatgcc aaatcgtggc ccctatggcc ctgggatacc ccaaatctat tcccagtcca
     4201 tccgaacgaa aggcggagat tctcaaaata atctcttaat cattggcctt tcatccactc
     4261 cccccttgat tccaacggga cgagggaagc tgtttcggcc tcctattatg aaagaacacg
     4321 accgtccttt ttctaggcaa ggacccggcc gcctcccggg gctcatgctc ttcttcacct
     4381 tcttcatcgg cttcatcctt taccttctcc tcttcgccac cgcccccatg gtatccatca
     4441 tcatggagga gatgaaccta tcccatgcgg aatttggtat ggttt
//
LOCUS       DASPRR010000455         6068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1236078, whole genome shotgun sequence.
ACCESSION   DASPRR010000455 DASPRR010000000
VERSION     DASPRR010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236078"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VEL68_16665"
     CDS             <1..303
                     /locus_tag="VEL68_16665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXZ37657.1"
                     /translation="KPGTGKKPAATDTVTTHYKGTLIDGTEFDSSYRRGKPATFPVSG
                     VIPGWTEALQLMEEGAKWQLFIPSNLAYAERGAGQVIGPHATLIFEVELISIQEKK"
     gene            300..749
                     /locus_tag="VEL68_16670"
     CDS             300..749
                     /locus_tag="VEL68_16670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ37658.1"
                     /translation="MSAPGPIPDYRFQPMEKGDLAEVIQFWQGMEGIGLNESDSYSAL
                     SFYLQRNPGLSWVARNKDRELIGTVLCGHDGRRGYLHHLAVAQDYRSKGIGRALVERC
                     LSSLRELKILKGNVFLFSHNPEGQGFWKAMGWKKREDLILMQRVLSL"
     gene            784..1812
                     /locus_tag="VEL68_16675"
     CDS             784..1812
                     /locus_tag="VEL68_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXZ37659.1"
                     /translation="MKAMVLKEIGSMEKNKTPLRLVDLPIPEPGEKEVLVRVSRCGVC
                     HTELDEIEGRTPPPRLPVILGHQVVGRVEKEGARVTHFKAGDRVGVGWIYSACGTCEF
                     CREGNENLCPEFRATGRDVNGGYAQYMTVSEDFAYRIPPAFSDSEAAPLLCAGAIGYR
                     SLRLTGLKDGQNLGLTGFGASAHLVLKMARHRFPNSRIFVFARSEKERTFAQELRAAW
                     AGDPSENPPEKLDCIIDTTPAWKPIVEALKNLRPAGRLVINAIRKEEGDKEVLRQMDY
                     PLHLWLEKEIKSVANVARRDVREFLDLAAQIPIKPEVQEFALEGANHALIELKERKVR
                     GAKVLRIG"
     gene            2122..3327
                     /locus_tag="VEL68_16680"
     CDS             2122..3327
                     /locus_tag="VEL68_16680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXZ37660.1"
                     /translation="MSKNGMGRRDFLKSSAAGLGGFIYLSSNEKSSPEKEQQKKDEKI
                     TYRTLGKTGIKLPVITMGVMNTSNPNLVRAALSNGLYFLDTAQTYQRGTNESMIGEVL
                     QGRPRDSFAIATKARLGIDQKTGLYTEDATEQAYAQKIDTSLKSLGLDYIDIYHHHGV
                     WVKETALYEPVLNALAKAKKAGKIRFIGITTHRNEPEIINAAVDSKVYDVVLPAYNFR
                     QKHAEEVKKAIARAAEAGLGVIAMKTIGGNVGGNYHNTQIDAPMALKWALQDPNVHTI
                     IAGFTTFDQLELDLKVLREPSLSKSEKETLRWAALQSPLYCQGCGKCLGQCVQQLPIP
                     DLMRAYMYLYGYRNLVEAQDLLFSLSLPAGLCGDCGSCPVKCLNGWNVSERIQNIVRL
                     QEVPSEFIA"
     gene            3349..4242
                     /locus_tag="VEL68_16685"
     CDS             3349..4242
                     /locus_tag="VEL68_16685"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HXZ37661.1"
                     /translation="MIRAKKRRSIHLLFFLSWALGCTIFGGGTMAEGKTLAFPEMSGW
                     KQDGKTQVFSPKTLYEYINGAADLYLAYEFQDLQVAEYKGEKKAGVTVEVYRHKDPTQ
                     AFGIYSQERLANARFLDIGAQGYHEPNVLNFVTGPYYVKINGFNTGEDDEKVLMPFAK
                     KVEEVLGEKTSLPRILSSFPQEGKKKNSEKFISKNFLGYSFFHSGYTADYEVSGKKFK
                     IFVIEGENQEDCRAMMEKYLKQTGNEGRKPSEGTYRIKDNYHGEVDLFWKGKFIWGIV
                     DLKDSDLRSKYLRAIEELGER"
     gene            4286..4747
                     /locus_tag="VEL68_16690"
     CDS             4286..4747
                     /locus_tag="VEL68_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HXZ37662.1"
                     /translation="MIIDRLEKASLYQGVHKRLAAAFDYLQKTDLDKVEPGGTHEIEG
                     RKVYVMVQQYETKPIEKGRWEAHRKYIDVQYVHQGAERFGYANLPELKPGNYDEAKDF
                     LSLEGEGKGDFFLVRQGTFVILFPQDAHMPGMAVSTPRTVKKFVVKVALGD"
     gene            4922..5713
                     /locus_tag="VEL68_16695"
     CDS             4922..5713
                     /locus_tag="VEL68_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ37663.1"
                     /translation="MADYSQIDRSPLLSFLFYPRRESKACPQGAFELLVPVEEGISVG
                     CRFYVGHRSWPWILFFHGNGEVASDYDEIAPFYHQGEVNLVVADYRGYGASGGSPNFT
                     HLIQDAHLLFTAVREELSHRNFDKGLWVMGRSMGSLSAVDLAYHYPDQMRGMLIESGF
                     ASVTRLIEHLNLPARGLKLEPIEQERRAMIRQIAVPTLILHGELDSLVPLQEAKDLYH
                     MLNSSKKRLVVIPHADHNTIMFAGLKQYFQEVQDFIKRTKGVKVS"
     gene            5933..>6068
                     /locus_tag="VEL68_16700"
     CDS             5933..>6068
                     /locus_tag="VEL68_16700"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXZ37664.1"
                     /translation="MIYVVATIELAEGKRDDFLKRVRQLVPEVRAEKGCVEYGPAIDT
                     S"
BASE COUNT         1522 a         1526 c         1780 g         1240 t
ORIGIN      
        1 aagccgggga ccggaaagaa gcccgcggca accgatacgg taacgaccca ttataaaggg
       61 actctgatcg atgggaccga gtttgacagt tcctaccgac ggggaaagcc ggccaccttt
      121 ccggtgagcg gagtgattcc gggatggacg gaagctttgc agctcatgga ggaaggggcg
      181 aaatggcagc tctttatccc ctcgaacctg gcctacgcgg aacgcggggc cggccaggtc
      241 atcggccccc atgccacttt gatttttgaa gtcgagttga tctctattca ggaaaagaaa
      301 tgagcgcccc cggaccgatt cccgattacc ggtttcagcc catggagaaa ggcgacctgg
      361 cggaggtaat ccaattctgg caggggatgg agggaatcgg attgaacgaa tcggattcct
      421 attccgccct ctccttttat ctgcagcgaa atcccggact gagttgggtt gcacgaaata
      481 aggaccggga gctgatcggc accgtcctct gcgggcatga cggccggagg gggtatcttc
      541 atcatctggc ggtggctcag gactaccgca gtaagggaat cggcagggct ttggtggaga
      601 gatgcctttc gtccctgcgg gagttgaaaa tcctgaaagg caatgttttc ctcttctccc
      661 acaatcccga agggcaagga ttctggaagg cgatgggctg gaagaagaga gaagacctga
      721 ttttgatgca gagggtcctg agcctttagg cagttcttgg gagaagcatc cctgggggcc
      781 ttcatgaaag ccatggtact gaaagaaatc ggcagcatgg agaagaataa gacccccctg
      841 cggctggtgg acctgccgat tccagaaccg ggggaaaaag aggtcctcgt ccgggtatcc
      901 aggtgcggag tctgccatac tgagctggat gagatcgagg ggcgcactcc tcctccccgc
      961 ttgcctgtga tccttggcca tcaggtcgtc ggaagggtcg agaaggaggg ggcgcgggta
     1021 acccatttca aagcgggaga cagggtggga gtaggctgga tttactcagc ctgtggaacc
     1081 tgcgagttct gccgcgaggg aaatgaaaac ctttgtccgg aatttcgggc taccggaaga
     1141 gatgtcaacg gcggctatgc ccagtacatg accgtatccg aagattttgc ctaccgaatt
     1201 cctcccgcct tctccgattc agaggcggcc cccctccttt gcgcaggggc gatcgggtac
     1261 cgctccttac gcctgaccgg cctcaaagac gggcagaacc tgggcctgac cggtttcgga
     1321 gcctcggccc acctggtttt aaaaatggcc cgccaccggt ttcccaactc cagaatcttc
     1381 gtcttcgcca ggagcgaaaa agagaggacc tttgcccaag aattaagggc ggcatgggct
     1441 ggagacccct cagaaaaccc accggaaaaa cttgactgca tcatcgatac aactccggcc
     1501 tggaagccga tcgtggaggc cctgaaaaat ctccgaccgg caggacggct ggtgatcaat
     1561 gccatccgga aggaggaggg ggacaaagaa gttctccgcc aaatggatta tcccctccac
     1621 ctatggttgg aaaaggaaat caaaagcgtg gccaacgtcg ctcggagaga cgtcagggaa
     1681 tttttggact tggcagccca aatccccatc aaaccggaag tgcaggagtt tgctctggaa
     1741 ggggccaacc atgctttgat tgaattgaag gaaaggaagg tcaggggagc gaaggtctta
     1801 agaatcggct gaacatctga agggggaatt ctcgaaggac cccccaattc ggtggcattt
     1861 ttcaaattca tattcccgcc caagttatcc cttggaggct ataccgaaat aaaatcttga
     1921 tagaaatggg ggggaatggt aaattgttcc gtagcccatt tttgaacccc tgcctgaact
     1981 tccggatttc aagaaatgat gagaaaacga aagggggatt gctctttttc cttgactgga
     2041 aatggggctt ctttttttaa gggcggaaag ctgtcggtcg gagagtacgg ttaagcgcct
     2101 aatagaaagg gagggtttgg gatgagcaag aacggaatgg ggagaaggga ttttttgaag
     2161 tcttcggcag cggggttggg gggcttcatt tatttaagct cgaatgaaaa aagttctcca
     2221 gagaaggagc agcagaagaa ggacgagaag atcacctatc ggactttagg caagaccggg
     2281 atcaaacttc cggtcatcac catgggggtc atgaacacca gcaatccgaa cctggtccgc
     2341 gcggccttga gcaacggcct gtactttctg gacacagctc agacctacca gcgggggacc
     2401 aatgaatcca tgatcggcga agtccttcag gggcgtcccc gcgattcttt cgccatcgcc
     2461 accaaggccc gcctgggtat cgaccagaag acgggcctct acacggaaga tgccacggaa
     2521 caggcctacg cccagaagat cgacaccagc ctaaagagcc tgggactgga ttacatagac
     2581 atttaccacc accacggcgt gtgggtgaag gagaccgccc tgtatgagcc cgttttgaat
     2641 gctctggcga aagccaagaa agccggaaaa atccgcttca tcggaattac gacccaccgc
     2701 aacgaacccg agatcatcaa cgctgcggtg gactccaagg tgtacgacgt ggttctcccg
     2761 gcttacaatt tccggcagaa gcatgccgag gaggtcaaga aggccattgc ccgggcggca
     2821 gaggcaggtt tgggggtcat cgccatgaaa accatcgggg ggaacgtggg agggaactac
     2881 cacaacactc agatcgacgc tccgatggcc ctgaagtggg ccttacagga ccccaatgtt
     2941 cataccatca tcgccgggtt caccaccttt gatcagctgg aattagacct aaaggtcctg
     3001 agagaacctt ccctttcaaa gtcagagaaa gaaaccctcc ggtgggcggc cttgcagtcg
     3061 cccctgtact gccagggatg cggaaaatgt ctaggccagt gcgtgcagca acttcccatt
     3121 cccgacctga tgcgagccta catgtatctt tacgggtacc gcaatctggt agaagcccag
     3181 gacctcctat tctcccttag cctgcccgcc ggcctctgcg gagactgcgg cagttgcccg
     3241 gtcaagtgcc tgaacgggtg gaatgtgtcc gagcggattc aaaacatcgt ccgcctccag
     3301 gaggtcccct cagaattcat tgcctgaaaa gaaggagaag ggatcaaaat gataagggct
     3361 aaaaaaagaa ggtctattca cctgcttttc ttcctatcct gggccctggg atgcacgatt
     3421 tttggcggcg gaacgatggc ggaggggaaa acccttgcct ttccggagat gagcggatgg
     3481 aaacaagacg ggaaaaccca ggttttctcg ccaaagacgc tctacgagta catcaatgga
     3541 gcagcggacc tctacctggc ctatgagttc caggatttgc aggtggccga atacaagggg
     3601 gagaagaaag ccggggtgac tgtggaagtg taccgccaca aggaccccac ccaggcgttc
     3661 gggatctaca gccaggagcg gctggccaac gccaggtttc tggacatcgg cgcccagggt
     3721 tatcacgaac ccaacgtgct gaattttgtg accggccctt attacgttaa gatcaacgga
     3781 tttaacaccg gtgaggatga tgagaaggtc ctgatgccct ttgcgaagaa agtggaggag
     3841 gtcctggggg aaaagacttc tctgccccga atcctttctt ctttccccca ggaaggaaag
     3901 aagaagaata gcgaaaaatt catttccaag aacttcctgg gttattcctt cttccactct
     3961 ggctacacgg ccgactatga ggtgtccggc aagaaattca agattttcgt catcgaggga
     4021 gaaaatcagg aagactgccg agcgatgatg gaaaagtacc tcaagcaaac ggggaatgaa
     4081 ggccggaagc cgtccgaagg aacctaccgg ataaaagaca actatcacgg cgaggtggac
     4141 cttttctgga agggaaaatt catctggggg atcgtggacc tgaaagattc ggacctccgg
     4201 tccaaatacc tcagagcgat cgaagagctg ggagagagat aagagaatcg gagggggaga
     4261 agaggaaggg aaagggagag aagagatgat cattgaccga ttggagaagg catctttata
     4321 ccagggggtt cacaagaggc tggccgcggc ttttgattat ctccagaaga ccgacctgga
     4381 caaagtcgaa ccggggggga cccatgaaat cgagggccga aaggtctacg tcatggtcca
     4441 gcagtatgag accaagccca tagaaaaggg gcgctgggaa gcccaccgga agtatatcga
     4501 tgttcaatat gttcaccagg gagcagagcg attcggctac gccaacttac cggagctgaa
     4561 accagggaat tacgatgaag ccaaagactt cctatcgctg gaaggcgaag ggaaggggga
     4621 tttcttcctg gtccgccaag ggacatttgt gattcttttt ccccaggacg ctcatatgcc
     4681 cggaatggcc gtctccactc ctcggacggt gaagaaattc gtggtgaagg tggccctggg
     4741 ggattaagag ggcctcaata gccttacata agataaagtg ctgatcttaa aatacggcgc
     4801 acgaatacag gcgcaaaaaa tcgatttgca caagaatgta gatgggttcc ccgaactcga
     4861 cgtttaaagg ctctccccgc aagactggac agcggcacgt ttccgcatga ggggagtact
     4921 gatggctgac tattctcaaa tcgaccggtc gcctctttta tcctttcttt tctatccccg
     4981 aagagaatcc aaggcctgcc cgcagggggc cttcgaactg ctggttccag tggaggaagg
     5041 aatctcggtc ggctgccgat tttatgtcgg gcaccggagc tggccgtgga ttctcttttt
     5101 ccatgggaat ggagaagtgg cgagcgacta tgatgaaatc gctcctttct accatcaggg
     5161 ggaagtcaac ctggtggtag cggactaccg tgggtatggg gccagcgggg gctctcccaa
     5221 cttcactcac ctaatccagg atgcccacct gttattcacg gccgtccggg aagagctttc
     5281 tcatagaaat ttcgacaagg gtctgtgggt aatggggagg tctatgggga gcctgtcggc
     5341 tgtggaccta gcctatcatt acccagacca gatgagaggg atgttgatcg aaagcggctt
     5401 tgccagcgtt acccgcctca tcgagcatct aaaccttccc gcccggggtc tcaagctgga
     5461 gcccattgag caggagcggc gggccatgat ccggcagatc gccgttccga ccctcatcct
     5521 ccatggagag ttggatagcc tggtccccct gcaggaagcc aaggaccttt accatatgct
     5581 aaattcatcg aagaaaagac tggtcgtcat tccccatgcc gatcacaaca ccatcatgtt
     5641 tgccggtttg aaacagtatt ttcaggaggt ccaggatttt atcaaacgaa ccaaaggggt
     5701 gaaggtttcc taagcggcag acctccgccc tgcaccaggg cagcataggg cccgcgccat
     5761 cgcagcctgc ctgccgccct ttttccaggg gagtgggggg atttcaacga ttcatttcca
     5821 attcggtttg actggggaca accggaaatc agattcccgg gggaaataaa tggagccttg
     5881 caatctttcc tcttgcggga atgactctgg acgatgaaag gagatggagg tcatgatcta
     5941 tgtggtcgcc acaatcgagc ttgctgaagg gaagcgcgac gatttcttaa agagagttcg
     6001 acagctggtc cctgaggtcc gggcggagaa aggctgcgtt gaatatggac cggcgatcga
     6061 tacttcga
//
LOCUS       DASPRR010000456         6343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_255085, whole genome shotgun sequence.
ACCESSION   DASPRR010000456 DASPRR010000000
VERSION     DASPRR010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255085"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VEL68_16705"
     CDS             <1..621
                     /locus_tag="VEL68_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37665.1"
                     /translation="FFRIRWLLRFPPGRERQTPSGPPDTSSLKGIVYSWANIAMPWSM
                     ESTRQEFFFYAQFMIFHLAVAANIIMSFVIPYAPFLMKPAIVMRGLQVLFALGCMIGC
                     YRLYRRLSNPFIRQISSPDDFFSLILLTVWMFVSIWAAPNRPEQGEGALMTYFIMTAF
                     FLVYVPFSKISHYLYYPFSRYYFGKTMGYRGVYPIKRGPEPKPTKI"
     gene            655..2412
                     /locus_tag="VEL68_16710"
     CDS             655..2412
                     /locus_tag="VEL68_16710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ37666.1"
                     /translation="MPAEEKTRSHKVKKVIERMGKKLNRQMVGSLVACVHCGMCTQSC
                     HYVLANPDDPTFAPAYKADQIRRIFKRHFDWTGKFFPWWVKAQDVFADEELEKLKDIV
                     FGKCTNCRRCSINCPMGVDFATLNRMARGLLTAVGVMPEGVAVVSKDQWEIGNQMGVL
                     PQDYLETLEWLSDELENEFKDPAARIPIDKKNADVVYAINPREIKYDPRTISDAARIF
                     YRAGENWTMASDGWDMTNFGLFSGDDELGASVVRKLFGKVIELGGKKLVLSECGHGYR
                     ATRCEGPNWAGMEVPFEMESSVLTMLRYIKEGRIQVGKTKNSLPVTFHDSCNNARSCG
                     FFEEPRELLNLLATDFREMYPNRAENYCCSGGGGAMSMSEYTPLRLKAGKVKADQLKA
                     TGAKIVVTTCHNCVDALTDVIRHYKLGMKVAQLVNLVANALVIEEKIIAVPEVLQPAV
                     LLQLKGYKILIVDDEPDILTFISTVLEDQGAVAIQASDADQALELALKEKPDLITLDL
                     SMPGKNGGFVFEELRKSPELSSTKVCIITGKPELRRLIYDRPVPPPEGYLDKPINEES
                     LLMNVRKILETPHDGKKTH"
     gene            2554..3987
                     /locus_tag="VEL68_16715"
     CDS             2554..3987
                     /locus_tag="VEL68_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXZ37667.1"
                     /translation="MDLNYKQFFEVMPAYLTILDRDLRIIQANQRFRKDFGDFEGRFC
                     YEINRHRTEPCQNCPARRTFEDGLRHGIEEQFKKVNGQEVALIVYTTAIRNEDGAITA
                     VMKMATDITEIKLLQNQLRESRERYRHLFEEVPCYITIQDRDLRIVEANRRFREDFGD
                     VVGNKCYQVYMHRSEECVPCAVKETFQDGQVHQSEEVVTARSGEQMNVLVSTAPIRST
                     DGQIKSVIEMSTDISPIRQLQSQLTSLGLVISSISHGIKGLLTSLDGGIYLVNSGMEK
                     NNPARLKQGWEMVERNIARIRTMVLDLLYYAKDREPALQWFPAQSVLEEVCGVVRGKA
                     KEVGVEVLTDIDPVAGDLLADPKAVRSLMVNLVENAIDACRVDKKKGSHQIRLGVRGY
                     PDSIEFEVSDDGIGMDQETREKAFSLFFSSKAGDGTGLGLFISNKIVQSHQGKIQLES
                     EPNKGTRVTIKLPRRGPMSRGASSKPS"
     gene            3999..4385
                     /locus_tag="VEL68_16720"
     CDS             3999..4385
                     /locus_tag="VEL68_16720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ37668.1"
                     /translation="MSSRKKILIVDDEEDILTYLSTLLADQGFETLKAKDGEEAWKQV
                     EANPPDLITLDISMPEKSGIKFYREMKTADRWKKIPIIIVTGVSEDMRKFLSSRHQIP
                     PPEGYLAKPVNREEILTLIQKLTTIP"
     gene            5562..6065
                     /locus_tag="VEL68_16725"
     CDS             5562..6065
                     /locus_tag="VEL68_16725"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXZ37669.1"
                     /translation="MKRIILIGLSLILAMALVAAVGLAQGPGFGRGFGGGLPISNLTP
                     EQSAKIQTLQQDHLKAISPLQQELLAKGTEMRSLSLNPNADPAVIGAKQKEIWEVRSK
                     LQEMTNNFRLQIRGVLTAEQQAQFDSFGPRRGMGPRMGLGPGAGFGTGGFGPGGGFGP
                     GVGLQNQ"
     gene            6126..>6343
                     /locus_tag="VEL68_16730"
     CDS             6126..>6343
                     /locus_tag="VEL68_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37670.1"
                     /translation="MKSLKIISFLLLASFFFFTGRCVKSSEAQPPFISPGSQPPQTEL
                     KGSCRKDPSITFTEEQAKEVVRLQQSFL"
BASE COUNT         1546 a         1721 c         1671 g         1405 t
ORIGIN      
        1 tttttccgta tccgctggct gctacgtttc ccccccgggc gagagaggca aacgcccagc
       61 gggccgccgg atacctcctc cttaaagggg atcgtctatt cctgggccaa catcgcgatg
      121 ccctggtcca tggagagcac ccggcaggaa ttcttcttct atgcccaatt catgattttc
      181 catctcgctg tggccgccaa tatcatcatg tcctttgtca ttccctatgc ccccttcctg
      241 atgaagcccg caattgtcat gcgggggctc caggtcctgt tcgctctggg atgcatgatc
      301 ggttgctacc gcctgtaccg gcggctgagc aatcccttca tccgccagat tagctctccg
      361 gacgatttct tctccctgat cctgctcacc gtgtggatgt ttgtttccat ctgggctgcc
      421 cccaaccgtc ccgagcaggg agagggagcg ctgatgacct atttcatcat gactgccttt
      481 ttcctggtgt atgttccctt cagcaaaatt tcccattatc tttactaccc ctttagccgg
      541 tactatttcg gcaagaccat gggctaccgc ggggtttatc cgatcaagcg gggacccgag
      601 cccaagccga ccaaaatata gccgaagaaa cggatgaatc gcgaggaaaa agaaatgcca
      661 gcggaagaga aaaccaggtc ccataaagtg aaaaaagtca tcgagcggat ggggaagaaa
      721 ctcaaccggc agatggtcgg ctcccttgtg gcctgtgtcc actgcgggat gtgcactcaa
      781 tcgtgccact atgtcctggc caaccctgat gatcccacct tcgccccagc ctacaaggcg
      841 gaccagattc ggagaatctt caaacgccat tttgactgga ccggaaaatt tttcccctgg
      901 tgggtcaagg cccaggacgt gttcgcggat gaagagctgg agaaattgaa ggatatcgtc
      961 ttcggcaaat gcaccaattg tcgaaggtgt tcgatcaact gccccatggg ggtggatttc
     1021 gccacgctca accgcatggc gcggggacta ttgaccgcgg tgggagtgat gcccgaaggc
     1081 gtggccgtgg tcagcaagga tcagtgggag atcggaaacc agatgggggt tctcccgcag
     1141 gactacctgg aaaccctgga atggctttct gatgaactgg agaatgaatt caaagacccg
     1201 gctgcccgga tccccatcga caaaaaaaat gccgatgtgg tttacgcaat caacccccgg
     1261 gagatcaagt acgacccccg aaccatctcg gatgcagcgc ggatcttcta ccgggcaggg
     1321 gagaattgga ccatggccag cgacggctgg gatatgacca atttcggcct tttttccgga
     1381 gacgatgaac tcggggcttc cgtcgtacgt aagctcttcg ggaaggtcat tgaactcggg
     1441 ggaaagaagc tggtattatc ggaatgcggc cacggatacc gggccacccg ctgtgaaggc
     1501 ccgaactggg cggggatgga agttcccttc gagatggaaa gctctgtcct caccatgctc
     1561 cggtacatca aggaaggaag gatccaggtt ggcaagacca agaacagcct accggtcacc
     1621 ttccatgact cgtgcaacaa cgccaggagc tgcggatttt tcgaggagcc ccgggaactt
     1681 ctaaacctgc tggcgaccga tttcagggag atgtacccca accgggcgga gaactactgc
     1741 tgttcgggtg ggggaggggc catgtccatg tccgaataca cgcccctgcg cctcaaggcg
     1801 gggaaagtta aggcggacca gctgaaggcc acaggggcca aaatcgtggt caccacctgc
     1861 cacaattgcg tggacgccct gaccgacgtg atccggcatt acaagctggg gatgaaagtc
     1921 gcccagctgg tcaacctggt ggccaacgca ttggtgatcg aagagaagat catcgccgtg
     1981 cccgaggtcc tccagccggc cgtgctcctt cagctgaaag gatacaagat tttgatcgta
     2041 gacgacgagc cggacatcct gaccttcatc tccacagtcc tggaagatca gggagctgtc
     2101 gccatccagg cttccgacgc ggaccaagcc ctggaactgg cgttgaagga aaagcctgat
     2161 ctgatcactc tggacctatc catgccgggg aaaaatgggg ggttcgtctt cgaggaactg
     2221 aggaaaagcc cggaactctc ctccaccaag gtctgcatca ttaccgggaa accggagctc
     2281 cggcgcctga tttatgaccg gcctgttccg cctccggaag gctacctgga caagccgatt
     2341 aacgaagaga gcctgctgat gaacgtgagg aagattctgg aaactcctca cgatgggaag
     2401 aaaacgcatt agctgccggg tgccacgaca gcggcaccca atcatgagaa tcaaattatt
     2461 gcccttcccc ttgatggggg gagagtgggg gtgaattggc ttcccccctc ccctttattg
     2521 cctcccgcca gggtggggga aaaggttggt cggatggacc tcaactacaa acaatttttc
     2581 gaagtcatgc ccgcttacct caccatcctg gaccgagacc tgcggatcat tcaggccaac
     2641 cagcgatttc ggaaggactt cggcgatttt gaagggcggt tttgctacga aatcaaccgg
     2701 caccggacgg aaccctgtca gaattgcccg gcccggagga ccttcgagga cggcctccgg
     2761 cacgggattg aggagcaatt caaaaaggtc aatggtcagg aggtggccct gatcgtctat
     2821 acaaccgcca ttcgcaacga agacggagcg atcactgcgg tcatgaagat ggccacggac
     2881 atcactgaga tcaaactcct ccagaatcaa ctgcgggaaa gccgcgagcg ctaccgccat
     2941 ctcttcgagg aagttccctg ctacatcacc atccaggacc gggaccttcg gatcgtcgaa
     3001 gccaaccgga ggttccggga ggatttcggt gacgtggtgg ggaacaaatg ctaccaggtc
     3061 tacatgcacc gttcggagga atgcgttccc tgcgccgtaa aggagacttt tcaagacggg
     3121 caggttcatc aaagcgaaga agtggtaact gcacggagcg gggagcagat gaatgtccta
     3181 gtctcgaccg cccccatccg cagcaccgac ggacagatca aaagcgtgat cgagatgagc
     3241 accgatattt ccccgatccg ccagctgcaa tctcagctta cttctctcgg cctagtgatc
     3301 agctccatct cccacggcat caaggggttg ctcaccagcc tggatggcgg catctaccta
     3361 gtcaactcag gcatggaaaa gaacaacccg gcgcggctca aacagggctg ggagatggtg
     3421 gaacggaata tcgctcgtat ccggaccatg gtattggacc tgctctacta cgccaaagac
     3481 cgggagccgg cattgcagtg gtttccggct caatccgtgc tggaagaggt ttgcggggtt
     3541 gtccggggaa aggcaaagga ggtcggcgtc gaggttctga ccgatatcga cccggtggcc
     3601 ggcgatctgt tggccgaccc gaaagcagtg cgctccctga tggtcaacct ggtggagaat
     3661 gccatcgacg cctgccgggt ggacaaaaag aagggcagcc atcagattcg actgggagtc
     3721 aggggatacc cggactccat tgagtttgaa gtgtccgacg acggaattgg aatggatcag
     3781 gaaacccggg aaaaagcctt cagcctattc ttttcttcca aagcgggaga tggaacgggg
     3841 ctggggctct ttatctccaa caaaatcgtc cagtcccacc aggggaagat tcagctggaa
     3901 tcggagccga ataaagggac ccgggtgacc ataaaattgc cccggagagg acccatgtcc
     3961 aggggtgctt cgtccaaacc ttcctgaggg agggaatcat gtcttccagg aaaaaaattc
     4021 tgatcgtcga tgatgaggaa gatatcctca cctatctttc caccctcctg gcagatcagg
     4081 gatttgaaac cctcaaagcc aaggatggag aagaggcctg gaagcaggtt gaggcaaatc
     4141 cccccgacct catcaccctg gatatctcca tgcccgaaaa atcggggatc aaattttacc
     4201 gggagatgaa gaccgccgac cgctggaaga agattcccat cattatcgtg accggagtct
     4261 cggaagacat gcgcaaattc ctctcctccc gccatcaaat ccctccccct gaaggatacc
     4321 ttgcgaaacc tgtgaaccgg gaagaaatcc taaccctcat tcaaaaattg acaactatac
     4381 cctgatttcc ggcgttcttc tcgtttttcc cttcttccgc gcccacccgg gcttattttc
     4441 ggctgctttg atgctcattg ataaaaggac ctggttggtg ccccgaaaat gcctaagatt
     4501 tgaagtcttt cagtcatctc aaatcaagtt gcctattttt aaatatatcc atagcaccat
     4561 tgactcttac cggttaccgg tcgccatgaa aaaaaatggc ctcagggccc ctttttaaag
     4621 gctccataca aatcctgaag agcctactat cataaaccaa caaagcgata ggttcatgcg
     4681 cttgttgatc ttgctcaaag gcttaatgat gggtcattta cttcttcctg ttgtttgacc
     4741 gattctgcca atttgtacag atttcaattg gtaatcgcgg agagaagagg ttatggcctc
     4801 cttctattta tgtacccata aatacaaggg ggaagtctcc atgcaaattc atgtgcttcg
     4861 acaaaattgt cgccctgatt ttcacttgtc gaaacatttt cttctataaa ccaatgaaat
     4921 tattaaaatc cattgataat acttcgacga aattgtcgaa ttagtaaaag ggcctctgtc
     4981 ctcggtgtca gtcccaaaat caacaaaaga ttccgaaatt caatgtcatt ttaccgctcc
     5041 tgaagccgcg ggcataagaa atgctttaaa agattggtaa gaaattaaag gatttgaata
     5101 ccatgttgct ccgcttgaga catcgctcgt tcttaatttt tctaattctc ctcgtcgcgc
     5161 tactccttgg cccacccctc tccatggcca agaacctgcc gacgatctgt aatattttcg
     5221 ataagggcaa agccgagaaa tcgggacatt gtggccaccg ggccatgtct tcaaagattc
     5281 aggataagtc tccagatttc gaagcagttc tctttttcaa tctagatctc cgaaacagcg
     5341 gccttttgat catccaaagt gactccctct ctgtcctttt cccgtccgga accaatttcc
     5401 aatccaatcc cctccgctgc tgattttcct ttcccttcgg gggcgtaagc cccgcttcat
     5461 ttcgaccgtt ccagaaaagc tttctgagaa attaaaatat attcctatcc cggcataatg
     5521 ccggcatagg caaaatccta aagcgatgga ggtgaacatc tatgaagagg atcatcctca
     5581 tcggtctgag cctgattttg gcaatggcct tggtagccgc cgttgggctc gcccagggcc
     5641 cgggatttgg ccgcggcttt ggcgggggac tgcccatcag caatctgaca cccgagcagt
     5701 ctgctaagat ccagaccttg cagcaagatc acttgaaagc gatctcgcca cttcaacagg
     5761 agcttctggc gaaaggaaca gagatgcgaa gcttgtcctt aaatccaaac gctgacccgg
     5821 cggtgatcgg ggccaagcag aaggagattt gggaggttcg atccaaactc caggaaatga
     5881 ctaacaactt caggctgcaa atccgcggag tattgacggc agagcagcag gcccaatttg
     5941 actccttcgg gcctcgccga ggaatgggtc cccgcatggg tcttggtcct ggtgcaggat
     6001 ttggaaccgg cggttttggt ccaggcggag ggtttggccc tggggttgga ctacaaaatc
     6061 agtaaacctt aaatcagggc ggattcattt ccgccctgct cacctatcaa tcaagaggtg
     6121 ggacaatgaa atcattaaag atcataagtt tcctattact ggcaagtttc tttttcttca
     6181 cggggcggtg cgtgaaatct tcagaagccc agccaccttt tatttcgccg ggcagccagc
     6241 ccccacaaac ggaactgaag gggtcgtgtc gaaaggatcc ctccattacg tttaccgagg
     6301 agcaggcaaa ggaagtcgtg cgccttcagc agtccttcct gga
//
LOCUS       DASPRR010000457        20253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1882107, whole genome shotgun sequence.
ACCESSION   DASPRR010000457 DASPRR010000000
VERSION     DASPRR010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 20253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20253)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20253
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882107"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VEL68_16735"
     CDS             complement(<1..488)
                     /locus_tag="VEL68_16735"
                     /inference="COORDINATES: protein motif:HMM:NF015050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37671.1"
                     /translation="MNPVDPGGREVPPCGYGLLGLCCFDCLRGPCRISPFEKTQALCG
                     AEPDLLVAGNLCRLTAKEFASVLKSFTQAAGEFRKRNETIGSFSMALTEKLARKYKIP
                     LEELIPFLEKETTKLLDPLADQPTLIFSSLFPERISPPGYSECRCGPLQGELLALLGW
                     KD"
     gene            complement(475..972)
                     /locus_tag="VEL68_16740"
     CDS             complement(475..972)
                     /locus_tag="VEL68_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ37672.1"
                     /translation="MTRQIFFRPERCLMCLSCVLSCQLNSLGTRDARCLPPGKPLKRI
                     SPVFSRGTPWMWKCQQCSQAPCAEACISGSLRPKEGGKGVEHRSETCVGCGSCLLACP
                     LSIPALDREGGRMSKCNLCPEEQIPPCVKACQSQALVFQEVSRFSWGKKRRYLLQSRG
                     TDEPG"
     gene            complement(969..2855)
                     /locus_tag="VEL68_16745"
     CDS             complement(969..2855)
                     /locus_tag="VEL68_16745"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ37673.1"
                     /translation="MGTFFGGYAGQILYVDLSDGSLQKKPLERDFALKYIGGRGFSSR
                     ILWDELKPGIDPFSSANIVIFAAGPLNGTPTPSASRLTVAAKSPLTGALGDANSGGYW
                     APELKYAGFDAIVVKGISPEPVYLWVEDGKAELRKAKHLWGLGVHTVGDRLKEEIDDD
                     TIHILAIGPGGENQVRYACVLTDGESAGGRCGIGAVMGSKRLKAVVVRGSRDVGIADP
                     KRFKAALDAYRETLEGEAWTETLRRLGTPNLVAHRQKLGIWGAKNFQRGTIEGWEKIS
                     GEAFREKFLHKVMGCMGCLVRCRRYSIISQGPFAPTYTKGPEYDTINALGAKTYITDP
                     AVVLRAQYLCDEYGIDAQTAGSSIAMAMELYERGILKKDQVDGVDLVFGNGEALLYFI
                     EQIPYRRGFGDLLAEGTKIMGERLNAGYYAIHVKGLEVDATDPRGYVTRALTYSVATR
                     GSCHLRGFPYIDEFIKPEEAEAFFGTPAVSRLEALEGKGKMVAWSEDWVTLADLAGVC
                     KFAWYRSRNFPMLIKRGLELMNEILMAVTGLPFTPEALLRCGERVYNVEKLFNLREGF
                     GRESDYPPQRFFQEETPDGPSKGAKLNLADYDRVLDDYYEARGWDRKTGQPTPEKLRN
                     LGLP"
     gene            complement(3115..3609)
                     /locus_tag="VEL68_16750"
     CDS             complement(3115..3609)
                     /locus_tag="VEL68_16750"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ37674.1"
                     /translation="MSRKRVGYFENRQDPENICSRCRMCELVCTLQHHGVGNPRRARI
                     QVIQLGKGEDIPVTCLNCEEPPCMNICPTGALQRPEPEGMVKVQEDVCIGCAMCVNAC
                     PIGAMTLDPVEGVAAKCDLCQGDPQCVLYCPAKVLKLTDAGQMARHRMKGFAKFLQSA
                     GEDH"
     gene            complement(3611..3907)
                     /locus_tag="VEL68_16755"
     CDS             complement(3611..3907)
                     /locus_tag="VEL68_16755"
                     /inference="COORDINATES: protein motif:HMM:NF020702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ37675.1"
                     /translation="MKIEVEFSPAFQSRLSLERQILTLEEGRPTVKDLFDRLAQEHGG
                     KIRSLLFGPKEDEILSGLMVMINDRTFTGNALNQKEIRLEEGDRVNLLYFMSGG"
     gene            complement(4094..5284)
                     /locus_tag="VEL68_16760"
     CDS             complement(4094..5284)
                     /locus_tag="VEL68_16760"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ37676.1"
                     /translation="MTPIPTLDSSHYRRSIFLSYFCTLLFWGAVYIYMPILAPYAEVV
                     SGSLQSVGLVMGAYGLSQLILRIPLGVWSDRWRRRKPFILLGFIFDGLAALGLLLSTS
                     TVMLFFSVLTAGIAASMWVPFTVLFSSYFPVGQVVYSMSLILFCTRISQIITNYTGAA
                     MAEIWGWGAAFYGGVVLSLVGVLMAASLAEHRPEKSSAVSFQQLSLACRNRTLLLPSM
                     ICTLFQFANFSINYGFAPIFAQAIGASKAALGILFLCHMVPNSLTTLLSGTYIRRRFS
                     ERSILFAGFFLIAGSIFFIPFINRLGTLYMIQAINGIGSGMVFPLLMGLSIQSIPREQ
                     QATAMGVFQSLYAVGMSLGPILSGFVAQQWGLPSVFALNGLLCLFSAFFSFIKIPSAR
                     WSDS"
     gene            complement(5310..6029)
                     /locus_tag="VEL68_16765"
     CDS             complement(5310..6029)
                     /locus_tag="VEL68_16765"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXZ37677.1"
                     /translation="MHPIIFIISILLSLNIQIPLVLSSEEASLVEARKRMVERDLKGR
                     DIRDPKVLEIMGKVPRHLFVEPSLRSKAYADYPLPIGEGQTISQPYIVALMTQILKIQ
                     PGERVLEIGTGSGYQAAVLAELTDEVYTIEIMEALTQQAAQHLKQLGYDKVKVKYGDG
                     YFGWEEAAPFDAIIVTCAANHIPPPLIKQLKEGGRLIIPLGSTTYFQTLTLLTKKMGK
                     PDIQHLLGVAFVPMTGEALKK"
     gene            complement(6214..10449)
                     /locus_tag="VEL68_16770"
     CDS             complement(6214..10449)
                     /locus_tag="VEL68_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXZ37678.1"
                     /translation="MDKDGLHLGLDIGSVSAKTVLIDEDMNVVEEHYTRTKGQPLETV
                     LRVLTEILTRVPSERIQSVSVTGTAAKLIAELLGAEFVNEVIAQAKSTSHFHPEVKTV
                     IEMGGEDSKLLLIDTTPGNGDYRIQDFSMNTICAAGTGSFLDQQANRLHYTIEEFSQL
                     ALKSKTPPRIAGRCSVFAKSDMIHLQQGATPDYDIVAGLCYALARNYKSNIAKGKNMV
                     TPVSFQGGVAANLGMRKAFFDVLELKEGEFIIPKHFASMGAIGAAFLTMENPAKRKKF
                     EGVERLRDYVSRPVTSDKTLEPLSLSEHHKKESQWEPLRGTPDGKKIPAYLGIDVGSI
                     STNVVVTDENIKVLSKRYLMTAGRPIEAIRQGLKEVGDEVGHLVEIKGVCTTGSGRYL
                     TGDYVGADVIRNEITAQATAAAFIDPEVDTIFEIGGQDSKYISLEHGAIVDFEMNKAC
                     AAGTGSFLEEQAEKIGISIKEEFGNLALGCKCPVSLGERCTVFMESDLVHYQQKGAKR
                     DDLVAGLSYSIVLNYLNKVVGDKRVGNRIFFQGGTAFNKGVVAAFEKVTGKKIIVPEH
                     NEVTGAIGCCILARDENKSGKTNFKGWDLSNRPYELTSFECKECPNRCEIRRVTIQGE
                     KPLFYGSRCEKYDVDRTRKRRDDLPDLFKEREEALMASYVEEKPVPEDAPVIGIPRML
                     NFLETMPFWKTFFTALGFRLVFSDTTNKRLIAKGVEKIVAETCFPIKVAHGHLLDLMD
                     KGVDTIFLPSIITMPLSHPSLDRSFACPYVQAFPYSVKSAIDFKARGVKVLSPVIHLG
                     AERKTLEQSLVSMGKELGKDGDEILRAIDKAEAAQRSFFASMKRRGREVLQNLKEGEK
                     AMVIVSRPYNGCDPGVNLGLPSKLRDLGVIAIPMDCLPLDEMDMLDDWKDMYWKYGQK
                     IFSAAHIIRDDPRLYAIYITNFACGPDSFILHFFKEKMRGKPYLQIEVDEHSADVGAI
                     TRLEAFLDSLKNIRRDLQVEERKPKTIVVTHDANHRKVYIPYMAEGAHAIVGAFQACG
                     IDAEVTPKSNQETIYWGRKYTSGKECYPCILTTGDMVRVVKSPDFDRKKVAFFMPSGN
                     GPCRFGQYHRLHRLILDDLGYPDVPIYSPTQDENLYKDLHMMGKNFTRLGWQGMVAVD
                     LLEKKLRETRPYEVHAGDSDRVFQAYLKKAYEAVRDSQDNPDFCMEGLVQALQDARKE
                     FDALEIVNKGSKPRIGIVGEIYIRSNEFSNEFIVRSLEELGGEVWLPPISEWFLYLNF
                     TSRRYSMQNRRYGNFWKTYITELVQKRDEHKMDHIFDGNLVNHPEPSIKETLAAAKPY
                     LDDSFEGEAVLSIGKCDDYLKKGCSGLVNVMPFTCMPGTIVGAVMKRYREDHNTIPFL
                     NMAYDGQEETNTLTRLEAFMHQARQYQRQYSNK"
     gene            10973..12007
                     /locus_tag="VEL68_16775"
     CDS             10973..12007
                     /locus_tag="VEL68_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ37679.1"
                     /translation="MNDLKFSGEICDACKTYDCLTRCQYLKMDLPTAREERQRLLRGE
                     KSRVLEECVTCYACEEYCPYGNHPFYHLVDQQEKLGVWPVPQPITKQQVLMMAPRGKI
                     TPKKVQPPVLNMCYFPMLTGSIRGKLFEGASTIVGSDIFCNVMWLHFGKNSVIRERLP
                     EVIANIWDHYLKDSGVKELICFHDECYGTYTHLAPAFGIPVPFKPIHIFDYLNKRLDE
                     LKSQIKPLGQKVAYQRSCSNRLVPETQPWVDEIFRKIGAERVDRKYDRENALCCGTVF
                     RAQQQDDLADDVQKRNLNDMAAAGAKFCVFSCPACLFTLGESVLKRGIMPILMNDLCQ
                     MALGEGMKVG"
     gene            12012..13391
                     /locus_tag="VEL68_16780"
     CDS             12012..13391
                     /locus_tag="VEL68_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXZ37680.1"
                     /translation="MEDVYKELAQIVGPSYVSNQPEERFIYSRDPGTMEPCEPDFVVL
                     PGTTDEVQKIVLLANRKKIPLVPMGGGLVLSGLSRALKGGIVLDLKRMNRILEVNEKS
                     RYALVEAGTSQGMLQAYLQKNHPTLKHSIPDSPPMATIGGNVLIHGSGHLSHAAGFHS
                     DMLNGLEVVLPTGEVVKIGSCSTSPYWFGRAPLPDLAGLFLGWFGTTGVVTKLAIKLY
                     PNRPYQDVGVFVTEDPDLVPEVFYQATGVQVAEDITAWMTPKPEWAKGFQHININYAA
                     DTPEELIWKRNLIRASLKGLISRKVGGFLPLPPPMKKNFLEAPQTTLAQFADVRKGGG
                     FEYVGGIMPIDLFPKAYRVGIEVAARQGTTYSTGARIIGLGHCMMFFFAYPFNRADEA
                     DVDRARRALEETNREVLEMGGIPWKAEVPAQKQIIGRMDAGTLNLMDRIRTMLDPKGI
                     MNPGNWEVR"
     gene            13388..14545
                     /locus_tag="VEL68_16785"
     CDS             13388..14545
                     /locus_tag="VEL68_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ37681.1"
                     /translation="MMEFADILHRCFRCGYCKFPNNYQDFNCPSYSKFRFETYSPGGR
                     MWLLRAWLNGEMKSSARLAEILFSCATCGNCVEHCVFPKFKDDILHAFLAAREEMVNQ
                     GTVPPAVRDYLKTLQVHGNPYKLPRLEREKWKHGLNLESYSGQEYLFHAGCVGSLDER
                     GIKMARSVAGLLKKLGVSLGTLGIEENCDGNEALALGEKGVFQSLVEKNLQSFQRLGV
                     KKVITLTPHAYHAFKNDYPKDAYPLEVFHYTQILSAQAKKLSALPTPSSFRVTYHDPC
                     YLGRHNKEYQAPRKILKSISGLQLLEMGRAKENAFCCGGGGGNFFTELLGSGAESPAR
                     ARVREAADTNASVLAVACPNCAKMLDDAVKSENLEEKIVVKEISEIIQGAA"
     gene            14647..15162
                     /locus_tag="VEL68_16790"
     CDS             14647..15162
                     /locus_tag="VEL68_16790"
                     /inference="COORDINATES: protein motif:HMM:NF039354.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HXZ37682.1"
                     /translation="MRTTAELSRKEITDLLHKGWMSHDGMWFYHCLKEFGIEKANKLN
                     KAAIRSLAPLEMNRLKKLLGIERVETFEEFKHLFSCGFDLLIADFMNARMTFPEKNVF
                     HWEFVPHQCFAYKGMQNIGVIEDYECGVVYRVAGWIDSLGIQYSVFPRVFKCLMLTQG
                     SCAGDFHLELK"
     gene            complement(15189..17600)
                     /locus_tag="VEL68_16795"
     CDS             complement(15189..17600)
                     /locus_tag="VEL68_16795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXZ37683.1"
                     /translation="MKASKTWFLVALFLLASLALSSCTHLGLNRYQAEGEGTLPGLKE
                     PVTVVRDEKGMAYIYAKSPEDAMVAFGFTTAQDRLFQMELIRLFASGRISEMIGEKGE
                     AIDTRMRTIGFHRHAKRHVSIMDPETKNLMQKYIDGVNAFIQTRSGEYPLEYKLAGIK
                     PKPWELAESMAVFYLMSWESAANIETEIIAQMLVEKIGPEKAKEIFPLNINPDDEPRG
                     KASIHFPTSESARLDFASDKKLLAFLEDRPLRVGSNNWVTGPHLSSGGKPILSNDPHL
                     DPRTLPGPWYPCGIITPDFRWVGTGIPGIPAMVIGRNEHIAVGVTNAYGDAQDLYVET
                     IDPNNSDRYLEGDQSLPFEVLTEKLMIKDKKAPGGKREKEIKIRVTRRGSVVSGVLSG
                     FKTDKVITLRWAPFETMGPFIGADQIMAAKSAEEVRKALSKFDIMMLNFVFADKEGNI
                     GWIASGKLPIRSQGDGTVPYVVKDGKDNWVAWIPFEKNPQIQNPQKGWLGTCNHNTVG
                     KDYPYYYSSHLSPSYRYRRLIQLMEQPGKKSVEDHWQFQRDPMNLMAKEITPVMARAL
                     MAHPDTKELGEILSKWDYLDHTDQAAPTIFQTVYREFALLVFQDELGNDLAKAMLSVW
                     YFWQERLQQMILEGNSPWFDNTLTKDVKETRDDLFYKAAQIASEKLRSSLGADPKEWK
                     WGKVHTIEFVSPIRREGFGKGLLGDGPYPFQGSGETLHRAIFAFNDPFKVTYSASLRM
                     VADLSDDDKILAVLPGGVAGRIFHPHAKDQIKAFINGEKVYWWFSDKAIQEHKRKTLI
                     LNPLK"
     gene            17782..18549
                     /locus_tag="VEL68_16800"
     CDS             17782..18549
                     /locus_tag="VEL68_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ37684.1"
                     /translation="MEIKGKTALVTGGASGLGAAAVRRIVAAGGNAVILDVQEEKGKT
                     LASELGKTAFFCRTDVTNEESANQTIAKAKENYGGVHFCVNAAGTGWAERTVTKTGPH
                     TLSNFEKIIRLNLIGTFNVASKAAFAMSTNPPDANGERGVIVNVSSTAAFDGQIGQAA
                     YSASKGAVVGMTLPMARDLSSLGIRVVTIAPGLFNTPLLGLLPQDYIKALNESVPFPK
                     RLGDPEEFALLVEQIILNPYLNGEVIRLDGALRMAPK"
     gene            18677..19135
                     /locus_tag="VEL68_16805"
     CDS             18677..19135
                     /locus_tag="VEL68_16805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXZ37685.1"
                     /translation="MSGENSKAIAQDLIRRVLENSPFFRHLQLEFLEAAEGIGRLALK
                     VQPFHLNTQGIVQGGVLSSLADTAALLAIGTKLSLGQRPRTVQMDIHFLAPAQKGTLT
                     AVGRIAKMGSLVSVSDVEIQDETGKTVCMARCTSVLVNVAGDEKFIPKIE"
     gene            complement(19212..>20253)
                     /locus_tag="VEL68_16810"
     CDS             complement(19212..>20253)
                     /locus_tag="VEL68_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXZ37686.1"
                     /translation="DLEIYLKIIDRTLAQKEVDGIVFLHTFNATFEGPRSRELFQKVM
                     ELSQKYDKPVAIYVSTEDQEVNYLKRNFNYPIFTQVVETVRALGINRRHYAESQRLRQ
                     RAEIPSFPADKEKVRPLLQKARKENRDLFIHEAAEVLRVYGIPMVRGVPVSDEKEAVQ
                     AARQLGFPVAMKVISQEISHKSDVGGVQLNLRSEAGVADAYQDMLRRIRQEYPQAKIE
                     GVLIQSMVTGGRELIVGGRQDEQFGSVVLVGLGGIFVEVFGEVSVRVAPISHREAMEM
                     IDELRGAPIFKGARGLKPSDIQSVVDVLLRLSQLLSDFPEIREMDINPLRVFSIREGC
                     MALDVRMILNKN"
BASE COUNT         4547 a         5474 c         5665 g         4567 t
ORIGIN      
        1 tggtctttcc agcctagtag ggccaaaagt tcaccctgca gcgggccgca ccgacattcc
       61 gaataacccg ggggggaaat tctttcagga aagagagacg aaaaaataag tgtgggttga
      121 tcggccaagg ggtccagaag cttggtagtc tccttttcga gaaagggaat gagctcttct
      181 aaaggaattt tgtatttacg agctaatttt tcggtcaagg ccatagagaa ggaccctatg
      241 gtttcattcc ttttcctgaa ttctcctgcc gcctgggtaa aggatttcag gaccgaagca
      301 aattcctttg ccgtaagtcg gcaaagattt cctgcgacca gcaggtcggg ttccgctccg
      361 cagagggctt gggttttttc aaaggggctg attcgacagg gaccccgaag gcaatcgaaa
      421 cagcagagcc ctaaaaggcc atacccgcag ggaggaacct cccgaccccc tggatcaacc
      481 gggttcatcg gtccccctgg actgaagcag gtaacgcctc ttttttcccc aggagaatct
      541 ggagacctct tggaagacca aggcctgact ctggcaggct ttaacgcaag gggggatttg
      601 ttcttccggg caaagattgc acttgctcat ccgtccccct tcccggtcta gggcggggat
      661 gctaagaggg caggccagca ggcaggagcc gcatcccaca caggtttccg accgatgttc
      721 cactcccttt ccgccctctt tggggcggag gctgccggaa atgcaggctt cggcgcaggg
      781 ggcttgggaa cactgttgac atttccacat ccagggggtg ccgcgggaaa agacgggcga
      841 aattctcttc agcggttttc ccggtggaag gcaccgagcg tctctggtgc ccaaagaatt
      901 taactggcaa gatagaacac aggagagaca catcaggcag cgttcggggc ggaaaaaaat
      961 ttgtcgagtc atggcaaccc caaattgcgc aatttctctg gggttggctg tccagttttc
     1021 ctgtcccacc cccgggcttc atagtaatca tccaacaccc gatcatagtc cgccaagttc
     1081 agcttcgctc ccttggaagg gccgtcggga gtttcctctt ggaagaatcg ctgcggcgga
     1141 tagtccgatt cccgaccgaa cccttccctt aggttgaaaa gcttttccac gttgtatacc
     1201 cgctccccac agcggagcaa agcctcgggg gtaaagggaa gtccggtcac cgccatcagg
     1261 atttcattca tcagctccag acccctttta ataagcatcg ggaaattgcg agaccggtac
     1321 caggcgaatt tgcatacccc ggccaggtca gcgagagtca cccagtcttc gctccaggcc
     1381 accatctttc ctttcccctc cagcgcctcc aaacgggaga cggccggagt tccgaaaaaa
     1441 gcttccgcct cctcgggttt tatgaattcg tcgatgtagg gaaacccgcg caggtgacag
     1501 gagccgcggg tggccacgga gtaagtgagc gccctcgtca cgtatccccg ggggtctgta
     1561 gcatccactt ccaggccctt gacgtgaatg gcgtaatacc cggcattcag ccgttctccc
     1621 atgatcttcg ttccctctgc cagaaggtct ccaaagcccc gccggtaggg aatttgctcg
     1681 ataaaataga ggagcgcttc gccgttgcca aagacgaggt ccaccccgtc cacttgatct
     1741 tttttcaaaa tcccccgttc gtaaagctcc atggccatgg cgatggaaga gccggcggtc
     1801 tgggcatcga ttccgtattc gtcacagaga tactgtgcgc ggagaacgac cgcgggatcg
     1861 gtaatatatg tcttagctcc cagggcattg atcgtgtcgt actccggtcc tttcgtataa
     1921 gtgggagcaa atggtccttg ggaaatgatc gagtacctcc ggcagcgcac caggcagccc
     1981 atgcagccca tcaccttgtg caggaacttt tctcggaagg cttcgccgct gattttttcc
     2041 caaccttcaa tggttcccct ttgaaaattt ttcgcccccc agattcccag cttctggcgg
     2101 tgggccacca ggttaggggt tcccagccga cgcaaagtct ccgtccaagc ttccccctcg
     2161 agggtttccc gatacgcatc cagagcggcc ttgaagcgct tcgggtcggc gatgcccacg
     2221 tcgcgcgagc cacgcacgac cacggcctta aggcgcttcg aacccatcac cgcaccgatg
     2281 ccgcacctgc cgccggcgct ctccccgtcg gtgaggacgc aggcatagcg cacttgattt
     2341 tcccctccgg gaccgatggc taagatgtgg atggtgtcat cgtcgatttc ttccttgagt
     2401 cggtcgccga ccgtatggac tcccaggccc cagaggtgtt tggccttgcg tagttcggct
     2461 tttccatctt caacccaaag gtatacgggt tcggggctaa ttcctttcac cacgatggca
     2521 tcaaacccgg cgtacttcag ttccggagcc cagtatcctc cggaattcgc gtctcccagc
     2581 gctccggtca ggggagattt agcggccacc gtcagacgac tggccgaagg ggtgggtgtg
     2641 ccgttcaatg ggccagccgc aaagatgacg atgttggcgg aagaaaacgg atcgattccc
     2701 ggcttcagtt catcccacaa aatccgcgag ctaaatcctc tgccgcctat gtatttcagg
     2761 gcgaaatccc gctccagggg tttcttttgg agcgagccgt cactcaggtc cacatagaga
     2821 atttgtccgg cataccctcc gaaaaacgta cccatcttca atccttttat tccgggagaa
     2881 cctgacctgg catagctgaa accgaacaag tttgacgaat tgagaaatcc gaatatcgaa
     2941 atccgaaaca aattcaaaat tcaaaggttc aaaaacgcca aaccaagaaa agacttttga
     3001 atttggaaaa ttcgggtttg tttcgaaatt cggatttcgt gcttcgaatt tcggaccggc
     3061 gctttccatt ggaagttttg ttaccatctt gcgcacaatt tgattttaaa atgcctaatg
     3121 atcctcaccg gcgctctgta aaaacttcgc aaatcccttc atccggtgcc gcgccatttg
     3181 gcccgcatcc gttagtttga gaaccttggc cgggcagtag aggacgcact gcgggtctcc
     3241 ctggcagagg tcgcatttag cggcaacccc ttcgacaggg tccaaggtca tggccccgat
     3301 gggacaagca tttacgcaca tggcacatcc gatacagaca tcctcctgaa ctttcaccat
     3361 cccctcgggc tcggggcgct ggagggcccc ggtgggacat atgttcatgc aggggggttc
     3421 ttcgcagttc aggcaggtga ctggtatatc ctcccccttg ccgagttgga tgacttgaat
     3481 ccgagcgcgc ctgggattcc ccaccccatg atgttgcagg gtacagacca gttcacacat
     3541 ccggcaacga ctgcaaatgt tctccgggtc ctgacggttt tcaaagtagc cgaccctctt
     3601 tctgctcaac tcatcctccg gacatgaaat acagtagatt taccctgtct ccttcctcga
     3661 gccggatttc cttctggttc agggcatttc ctgtgaaggt ccgatcgttg atcatgacca
     3721 tcagcccgct taggatttca tcctccttgg ggccgaagag gagagatctg atttttcctc
     3781 catgctcttg ggctaaccgg tcaaagaggt ccttaaccgt gggccggcct tcctctagcg
     3841 ttaaaatctg gcgttcaagg gaaagtctgg attgaaaggc agggctgaat tcaacttcga
     3901 tcttcataaa tcctgggtcc agaatccatc ggcgcaggac ctattcgata aattccttgg
     3961 aactcattcc gttcaagatc ggtttactcg tactataaca cacaagtcag cctgtggcaa
     4021 aactcacttc cagccgaggg tccttccgaa ttgccgattg cagattgcgg aatgcggatt
     4081 gaaaaacgac aactcatgaa tcgctccacc gggctgaagg aatttttata aaagagaaaa
     4141 aggcgctgaa gaggcaaaga aggccattga gggcaaaaac ggaaggaagg ccccattgct
     4201 gggcgacgaa cccagatagg atcggcccca ggctcatacc cactgcatag agggattgga
     4261 aaacacccat ggctgtggcc tgctgttccc tggggatgga ttgaatggac aagcccatca
     4321 gaagaggaaa gaccatgccg cttccgatcc cgttgatggc ctggatcata tagagggtac
     4381 ctaaacggtt gatgaaaggg atgaagaaga tggatcccgc gatcaagaaa aatcccgcga
     4441 aaaggatgga acgctccgaa aaccggcgac ggatgtaagt tccggaaagg agcgtagtca
     4501 gagaattcgg aaccatgtgg cataagaaaa gaatgccgag ggctgctttg gaagctccga
     4561 ttgcctgggc gaagatcggg gcaaatccat agttgatcga gaaattagcg aactggaaga
     4621 gggtgcaaat catcgaaggc aaaaggaggg tccgattgcg gcatgcaagg gaaagctgtt
     4681 ggaaagaaac cgccgaactc ttttccgggc ggtgttcggc gaggctggca gccataagaa
     4741 cgcctaccag ggagaggacc acgcccccat aaaaagcagc tccccacccc cagatttcgg
     4801 ccatagcagc gccagtataa ttggtaataa tctgagatat acgggtacaa aagagaatga
     4861 ggctcatgga ataaacgact tgccccacgg gaaaataact cgaaaataag accgtaaaag
     4921 gcacccacat gctggcggcg attccagcag tcaggacgga aaagaagagc atgactgtac
     4981 tggtggaaag aagcaggccc aaggcagcca gcccatcgaa gatgaaacca aggaggatga
     5041 aaggttttcg tcttctccaa cgatctgacc agacccccag aggaatgcgt aggatcaatt
     5101 gtgacagtcc ataagccccc atcaccaggc cgacggattg cagcgagccg ctcaccactt
     5161 cggcgtaggg ggcaaggatc ggcatataaa tgtaaactgc cccccagaag agcagagtgc
     5221 aaaaatagga gaggaaaatg gaacgacgat aatgggagga gtcaagagta ggaatcgggg
     5281 tcataacaga tgggagagaa aaattcggat cattttttca gcgcctcgcc ggtcatggga
     5341 acgaaggcaa ctcccagaag atgttggatg tcgggcttcc ccattttttt ggtaagcagg
     5401 gttaaggtct ggaaataggt cgtgcttccc aaaggaatga tcagccgtcc cccctctttc
     5461 agctgcttga tcaggggagg ggggatgtgg ttggcggcac aggtgacgat gatggcatcg
     5521 aagggggccg cctcctccca gccgaagtac ccgtccccgt attttacctt gaccttgtca
     5581 taccccagct gcttcaggtg ctgggccgcc tgttgggtca gggcttccat gatttcaatg
     5641 gtgtacacct catcggtgag ttcggccagc acggcagcct ggtaacccga accggttccg
     5701 atctccaaaa cccgctcgcc aggctgaatc ttcagaatct gagtcatcag ggctacgatg
     5761 taaggctggg agatggtctg tccctctccg ataggcaggg gatagtccgc gtaggccttg
     5821 cttctcaggg agggttcgac gaagaggtgg cgggggacct tgcccatgat ttccaggacc
     5881 ttggggtcgc ggatgtcccg ccccttgagg tcgcgctcca ccatcctttt gcgcgcctca
     5941 accaggctcg cttcctccga ggagaggacg agaggtatct ggatgtttag cgaaagcaaa
     6001 atggagataa tgaagatgat cgggtgcatg ggggaaagac agcgatcagc tgtcagctaa
     6061 aggaaaatag caactgaatg ttattctagc ctaatttgcg gatcactgga aggggaaagc
     6121 agctaagaaa aaaggaagcg atcagccatc agcattcagc aaaaacccaa aagatttcta
     6181 gcattagctg ataattgacc gctgatcgct tcttcactta ttactgtact gccgttgata
     6241 ctggcgggcc tggtgcatga aggcctccag gcgggtcaag gtattggtct cctcctgccc
     6301 gtcataggcc atgttgagaa aagggatggt gttgtggtct tcccggtacc gtttcattac
     6361 cgcccctacg atggtcccgg gcatgcaggt gaagggcatg acgttgacca ggccggagca
     6421 gcctttcttc aagtagtcgt cgcattttcc gatggagagg acggcttctc cctcgaagga
     6481 gtcgtccagg taaggcttgg ccgccgccag ggtttccttg atcgaaggct cggggtgatt
     6541 caccaggttg ccgtcgaaga tgtgatccat cttgtgttca tcccgttttt gaacgagttc
     6601 ggtaatgtag gtcttccaga aattcccgta tctgcggttc tgcatgctgt agcggcggga
     6661 ggtgaagttt aggtaaagga accattcgga gatgggtggc agccagactt ctccgcccag
     6721 ttcttccagc gaccggacga taaactcgtt gctgaactcg ttggagcgga tgtagatctc
     6781 ccccacgatg ccgatgcgcg gtttggaccc tttgttgaca atctccaggg catcgaattc
     6841 tttacgggcg tcctggagag cctgaaccag cccttccatg caaaagtcgg ggttatcctg
     6901 gctgtcccgc accgcttcgt aggctttctt caaataggct tggaaaacgc ggtccgaatc
     6961 tcccgcatga acctcatagg gccgggtctc gcgcagcttc ttttccagaa gatcgacggc
     7021 caccatgccc tgccagccca ggcgggtgaa attcttgccc atcatatgca ggtctttata
     7081 gagattttca tcctgagtgg gagagtagat gggcacgtcg gggtatccca ggtcgtccag
     7141 gatcaaacgg tggagccggt ggtactgccc gaaccggcag gggccgttgc cggagggcat
     7201 gaagaaggcc actttttttc ggtcgaagtc cgggctcttc accaccctta ccatgtcgcc
     7261 ggtggtcagg atacaaggat agcattcctt gccggaggtg tatttccttc cccaatagat
     7321 cgtctcctgg ttggatttgg gagtcacctc ggcatcgatc ccgcaggcct ggaaggcgcc
     7381 gacgatggca tgggccccct cggccatata ggggatataa actttccggt ggttggcatc
     7441 gtgggtgacg acgatggtct tgggtttccg ctcctccact tggaggtccc ggcggatgtt
     7501 cttgaggctg tccaggaacg cttccaggcg ggtgatggcc cccacgtctg cgctgtgctc
     7561 gtccacctcg atctgaaggt agggcttgcc ccgcatcttc tccttgaaga agtgcaagat
     7621 aaaagaatcc gggccgcagg cgaagttggt aatgtagatg gcgtaaagcc ggggatcgtc
     7681 gcggatgatg tgcgccgcgc tgaagatctt ctggccgtac ttccagtaca tgtctttcca
     7741 gtcgtccagc atgtccattt cgtccaaagg aaggcagtcc atgggaatgg cgataacccc
     7801 caggtcacgc agcttggagg gaagccccag gttgacgccc ggatcgcatc cgttgtaggg
     7861 acggctgacg atgaccatgg ccttttcgcc ctctttcagg ttctgcaaga cttcccggcc
     7921 gcgccgcttc atggaggcaa agaagctcct ctgagccgcc tcggccttgt caatggcccg
     7981 caggatctcg tcgccatctt tgcccagctc cttgcccatg ctgaccagcg actgctccag
     8041 ggtcttgcgc tccgccccca ggtggattac cggggagagg accttcaccc cgcgggcctt
     8101 gaaatcgatg gctgatttga ccgagtaggg gaaagcctga acgtaggggc aggcgaagga
     8161 gcggtccagg gacggatggg aaaggggcat ggtgatgatg ctggggagga agatagtgtc
     8221 cacgccttta tccatcaggt cgagaagatg gccgtgggcc accttgatgg ggaagcaggt
     8281 ctcggccacg atcttctcca ctcctttggc gatgagacgc ttgttggtcg tgtccgaaaa
     8341 gaccaaacgg aaccccaagg cggtgaagaa ggtcttccag aagggcatgg tctccaggaa
     8401 gttgagcatg cgggggatgc cgatcaccgg ggcatcctcc ggtacgggct tttcctccac
     8461 gtaagaggcc atgagggcct cctcgcgctc cttgaagagg tcgggcaggt cgtcgcgtcg
     8521 cttccgggtc cggtccacgt cgtacttttc gcaccggctg ccgtagaaga ggggtttctc
     8581 cccttggatc gtgaccctgc ggatctcgca gcggttgggg cactccttgc actcgaaaga
     8641 ggtcagctcg tagggacggt tgctcaggtc ccatcctttg aagttggtct tgccggattt
     8701 gttctcgtcc cgggccagga tgcagcagcc gatggccccg gtgacttcgt tgtgctcggg
     8761 aacgatgatc ttcttcccgg tcaccttctc gaaagcggcc accacccctt tgttgaaggc
     8821 cgtgccgccc tggaagaaaa tcctgttgcc tacccgtttg tcccccacca ccttgttcag
     8881 gtagttgaga acgatggaat aggagaggcc ggccacaaga tcgtcccgct tggccccttt
     8941 ctgctggtag tgtaccaggt ccgattccat gaagaccgtg catcgctccc ccagggagac
     9001 agggcatttg cagcccaggg ccagattgcc aaactcttct ttgatggata tgcctatttt
     9061 ttcggcctgt tcctccagga aggacccggt cccggcggcg caggctttgt tcatttcgaa
     9121 atccacgatg gcgccgtgtt ccagggatat gtacttggaa tcctgtcctc cgatttcaaa
     9181 gatggtgtcc acttccgggt cgataaaggc agcggctgtg gcctgggcag tgatctcatt
     9241 gcggatcaca tcggccccca cgtagtcgcc ggttagatag cggccggacc cggttgtgca
     9301 gacccctttg atttccacca ggtggcctac ttcgtcgccc acttccttca gaccctggcg
     9361 gatggcctcg atgggacggc ccgcggtcat gagatagcgt ttggaaagga ctttgatgtt
     9421 ctcatcagtg accaccacat tggtggagat ggaacccacg tcgattccca agtaggcggg
     9481 gatcttcttt ccgtccggag tgccgcggag tggctcccat tggctttcct tcttgtggtg
     9541 ctcggaaagg gagaggggtt cgagcgtttt atccgaagta accggccggc tcacgtagtc
     9601 gcgcagccgt tccacccctt caaatttctt ccgcttggcc ggattttcca tggtcaggaa
     9661 agcggccccg atggccccca tggaggcgaa gtgtttggga atgatgaact cgccctcttt
     9721 cagctccagc acgtcgaaga aagccttgcg cattcccaag ttggcagcca ccccgccctg
     9781 gaaggagacg ggggtgacca tattctttcc cttggcgatg ttggacttat agttccgggc
     9841 cagggcatag cagaggccgg ccacgatgtc atagtcgggc gtggccccct gctgcaggtg
     9901 aatcatgtcg ctcttggcga agacgctgca ccggccggcg atccggggag gggtcttgga
     9961 cttgagggcc agctggctga attcttcgat ggtgtagtgg aggcggttgg cctgctggtc
    10021 caggaaggag cccgtgccgg cggcgcagat ggtgttcatg gagaagtcct ggatccgata
    10081 atcgccgttg cccggggtgg tgtcgatcag gaggagcttg gaatcctccc cccccatctc
    10141 gatgaccgtc ttcacctccg ggtggaagtg gctggtggat ttggcctggg cgatgacttc
    10201 attgacgaat tccgccccca ggagctcggc gatcagtttg gcggcggttc cggttacgga
    10261 aacgctctgg attctctccg agggaactcg ggtcaggatc tcggtcagga cccggaggac
    10321 cgtctccagg ggctgaccct tggtccgggt gtaatgctct tcaacgacat tcatatcctc
    10381 atcgattaaa accgtcttgg cgcttaccga tcctatgtcc aatcccaagt gaagtccgtc
    10441 tttatccatt ccagcgcaaa gcctccgtcg cagagaattc gcttccaata ccgattcaag
    10501 ggaggaaagc ggtgagaaag attggaaatt cagaatagga acctttcccg ccagacccaa
    10561 tgatttatat cataaatggg tccccggaga caacccgatc tttctttcaa tgataaaaac
    10621 gatccgctct acattatgga ggagaatcag aagccgattt tgaattcatc ttttcggcaa
    10681 gattttccca atccatcccc actcccttcg gcaaaaggga aacgcagccg gtttggtctt
    10741 gacaaaatga atggttaaat ctattgttca attgactttt cccgaccgaa gtcgtttctt
    10801 tccaagaaat agccattggg gtccataagg tttcagaatt ctggttgtct cgtccccaaa
    10861 tcctctcctt ttctatcggg gaggaaggat ggcagcgacc gatggcgaac caggtccttt
    10921 ttcccgtccc catgacttat ccacatgata agccccaaaa ggagccgcac gcatgaatga
    10981 tttgaaattc tctggggaaa tctgcgacgc ctgcaagacc tatgactgcc tcacccggtg
    11041 ccagtacttg aaaatggacc tcccgacggc cagggaggag aggcaacgcc tcctgcgggg
    11101 ggagaagtcc cgggtcctgg aggagtgtgt aacctgctat gcctgcgagg agtattgtcc
    11161 ctatggaaac catccttttt accacctcgt ggaccagcag gaaaagttgg gggtctggcc
    11221 ggttccccag ccgattacca agcagcaagt cctcatgatg gcccccagag gcaagatcac
    11281 ccccaaaaaa gtccagcccc cggttttgaa catgtgctac ttccccatgc tgaccggaag
    11341 catccgggga aagctcttcg agggggcttc gacgatcgtg ggaagcgata tcttctgcaa
    11401 cgtcatgtgg cttcacttcg ggaagaactc ggtcatccgg gaaaggctcc cggaggtgat
    11461 cgccaacatc tgggatcatt acctcaagga cagtggggtg aaggaactca tctgcttcca
    11521 cgacgagtgc tacgggacct atacccatct ggccccggcc ttcgggattc cggttccctt
    11581 caagcccatc catatcttcg attacttgaa caaaaggctg gatgaattga agagccagat
    11641 caagcccctc ggccagaagg tggcctatca gcgttcctgt tccaatcgct tagttccgga
    11701 aacccagccg tgggtggatg agatcttcag gaagattggg gcagagcggg tggatagaaa
    11761 atatgaccgg gaaaacgccc tatgctgcgg gacagtcttc cgggcccagc agcaggacga
    11821 cctggcggac gatgtccaaa agagaaacct gaatgacatg gctgcggcag gggccaaatt
    11881 ctgcgtgttc agctgcccgg cctgcctttt cactctgggc gaatccgtgc tgaagagggg
    11941 aatcatgccc atcctgatga acgacctctg ccagatggcc ctgggcgaag ggatgaaggt
    12001 ggggtgagtt tatggaagac gtatacaaag aacttgcgca gattgtgggc ccttcctacg
    12061 tatccaatca gccggaggaa cggttcatat actcccgcga cccggggacg atggaaccct
    12121 gtgagccgga ttttgtagtt ctgccgggca caaccgacga agtgcagaaa atcgtccttc
    12181 tggccaaccg gaagaagatt cccctcgtcc ccatgggggg agggctggtc ctctcaggcc
    12241 tttcgcgtgc cctcaaaggg gggattgtcc tggacttgaa gcggatgaac cgcattctcg
    12301 aggtgaacga aaagagccgc tacgccctgg tggaggcggg aacctcccag gggatgcttc
    12361 aggcatacct gcagaagaac catccgaccc tgaagcactc tattcccgac tcccctccca
    12421 tggccaccat cggcggtaac gtgctgatcc acgggtccgg gcacctctcc cacgcggctg
    12481 ggttccattc ggacatgctc aacggcctgg aggtggtgct gcctacggga gaagttgtga
    12541 aaatcggctc ctgctccact tccccctatt ggttcggccg ggcgcccctt cccgacctgg
    12601 ccgggctctt tttggggtgg tttgggacga cgggtgtggt gaccaagctg gccatcaagc
    12661 tctatcccaa ccggccttat caggatgtgg gggtttttgt caccgaagac ccggatctgg
    12721 tccccgaggt tttttaccag gctacagggg tccaggtggc tgaagacatc accgcctgga
    12781 tgacccccaa accggaatgg gccaaggggt ttcagcatat caacatcaac tatgccgcgg
    12841 atacgccgga agaactgatc tggaagagaa acctgatccg agcttctttg aaggggttga
    12901 tcagccggaa ggtgggaggt ttcctgcccc tgccgccgcc catgaagaaa aatttcctgg
    12961 aggctccgca aacaacgctc gcccaattcg ccgatgtccg caaaggaggg ggattcgaat
    13021 atgtgggagg catcatgccc atcgacctct ttcctaaagc ctatcgggtg gggatcgagg
    13081 tcgctgcccg gcaggggacg acgtactcca ccggagcccg aatcatcggc ctgggccatt
    13141 gcatgatgtt ctttttcgcc taccccttca accgggcgga cgaagcggat gtggaccggg
    13201 cccggagggc gctcgaagaa accaaccggg aagtcctgga gatgggcggg atcccctgga
    13261 aagccgaagt cccggcccag aaacagatca tcggaaggat ggacgcaggg actttgaacc
    13321 tgatggaccg tatccggacg atgctggacc ccaaaggcat catgaacccc ggcaactggg
    13381 aggtgagatg atggaattcg ccgatatcct ccaccggtgc tttcgctgcg gctactgcaa
    13441 gtttccgaac aactaccagg attttaattg cccctcctat tccaaattcc gctttgaaac
    13501 ctattctccc gggggccgga tgtggctgct gcgggcctgg ctgaacgggg aaatgaaatc
    13561 cagcgcccgc ctggcggaga ttcttttttc ctgcgccacc tgcggtaact gcgtggaaca
    13621 ctgcgtcttc cctaaattca aggacgatat tctccacgct tttctcgcgg cccgggagga
    13681 gatggtcaac cagggaactg ttcccccggc ggtgcgggat tacctgaaaa ctctacaggt
    13741 ccatggaaat ccctacaagc ttccccgatt ggagagggag aagtggaagc atggtttaaa
    13801 cctggaatcc tattccggcc aggaatatct atttcatgca gggtgcgtag ggtccctcga
    13861 cgaaagaggg ataaaaatgg cccgttcggt ggcgggcctc ctgaaaaagt tgggagtctc
    13921 tttggggacc cttggtatag aagaaaattg cgacgggaac gaggcacttg cgcttggaga
    13981 aaaaggggtt tttcaatccc ttgtggaaaa aaatctccaa tcattccaga ggctgggggt
    14041 gaagaaggtg atcaccctta ccccccatgc ctatcatgcc ttcaaaaacg attatcccaa
    14101 ggacgcctat cctctcgagg tttttcacta cacccagatt ctgagcgccc aagccaagaa
    14161 attatccgca cttccgacac cttcttcttt tcgagtcact tatcacgacc cctgttacct
    14221 cggacggcac aacaaggaat accaggctcc ccgcaagatt ctgaaatcta tctctggttt
    14281 gcagctgctg gaaatgggcc gggccaagga gaatgccttc tgctgcggcg ggggaggggg
    14341 gaatttcttt accgaacttc tgggaagcgg cgcggaaagc ccggcccggg cgagagtccg
    14401 ggaagccgcg gataccaacg cttccgtcct cgccgttgcc tgccccaact gtgccaagat
    14461 gctggacgat gcggtcaaat ccgaaaacct ggaagaaaag atcgtggtaa aagaaatttc
    14521 cgagatcatc cagggagccg catagcgcag aaagcatagc gcatggggtg aacaattttg
    14581 ctctcctctt agtccccccc tttataaaag gggggatggc ggggatttct ggaaacgaga
    14641 ttccctatga gaacaactgc tgaacttagt cggaaagaaa tcaccgacct tctgcacaag
    14701 ggctggatga gccacgacgg catgtggttc taccattgcc taaaggagtt cgggatcgag
    14761 aaggccaaca aactaaataa ggcggcgatc cggtcgctgg ccccgctgga gatgaacagg
    14821 ctcaaaaagc tcctgggaat cgaaagagtt gaaaccttcg aagaattcaa acaccttttt
    14881 tcctgcgggt tcgatttgct catagccgat tttatgaacg cccgcatgac ttttccggag
    14941 aagaatgtct tccactggga gttcgtaccc caccagtgtt ttgcgtacaa gggcatgcaa
    15001 aatatcggag tgatcgaaga ctacgaatgc ggcgtggtct atcgggtggc aggctggatc
    15061 gacagcctgg gaattcagta cagtgttttc ccccgggtgt tcaaatgcct tatgttgacg
    15121 caaggctcgt gcgccggtga ttttcatttg gaactgaaat gatttttctc attcaattca
    15181 ttcctttttc attttaaagg attaagaata agagttttcc tcttgtgctc ctggatggct
    15241 ttgtcgctga accaccagta caccttctct ccattgataa aagccttgat ctgatcttta
    15301 gcgtgggggt ggaaaatacg gccagcgact ccgcccggca gtaccgccaa tatcttgtcg
    15361 tcgtcgctta aatccgccac cattctcaga gaggccgaat aggtcacctt aaaggggtcg
    15421 ttgaaggcga aaatggcccg gtggagggtt tctccggaac cctgaaaggg gtatggccca
    15481 tcgcccagca aacctttgcc gaagccttct cggcgtatgg gactgacaaa ctcgatggta
    15541 tgcaccttgc cccatttcca ttccttggga tcggcaccaa gggaggaacg cagcttctct
    15601 gaggcgatct gcgcggcttt atagaaaagg tcatccctcg tttctttcac atccttggtc
    15661 aaggtattgt caaaccaggg tgagttccct tcaagtatca tctgctgcag cctttcctgc
    15721 cagaaatacc agacactcaa catggccttg gccagatcat ttcccagctc atcttgaaaa
    15781 accaacaggg cgaattcccg gtagacagtt tggaagatcg tgggggcagc ctggtctgtg
    15841 tggtccaggt aatcccattt ggagaggatt tctcccagct ctttggtatc cgggtgggcc
    15901 attaaggccc tggccataac cggggtaatc tctttggcca tgagattcat gggatccctc
    15961 tgaaattgcc agtggtcttc cacagacttc ttacctggct gttccattag ctggataagc
    16021 ctgcggtaac gataagaagg ggataagtgg gaggagtaat aataaggata atccttaccc
    16081 acggtattgt ggttgcaggt tcccagccaa cccttttggg gattttgaat ttgaggattc
    16141 ttttcgaagg ggatccaggc gacccagttg tcctttccgt ctttcactac gtaggggacg
    16201 gttccgtctc cctgggagcg gatgggaagt ttcccgctgg cgatccagcc gatgtttcct
    16261 tctttgtcgg caaaaacgaa gttcaacatc atgatgtcaa atttgctcag agctttgcgg
    16321 acttcttccg ccgatttggc tgccatgatt tgatcggccc cgatgaaagg gcccatggtt
    16381 tcaaaagggg cccagcgcag agtgatgacc ttatcggtct taaagccgga cagcactccg
    16441 gaaaccaccg atcctcggcg ggtcaccctg atcttgatct ccttttctcg ttttcctcca
    16501 ggtgccttct tatctttgat catcaatttc tcggtcagga cttcaaatgg cagggattga
    16561 tctccttcca aatatcggtc ggaattattg ggatcgatcg tctccacata gagatcctgg
    16621 gcatctccgt aggcattggt cacccccacg gcgatatgct cattccgccc aataaccatg
    16681 gcggggattc caggaatgcc cgtccccacc cacctgaaat ccggagtgat gatgccgcag
    16741 ggataccacg gaccgggcag ggtccggggg tcgagatggg gatcattgga caagatgggc
    16801 tttccgcccg aggaaaggtg cggcccggtc acccagttgt tgcttccgac ccgcaggggc
    16861 ctgtcctcca gaaaagccaa caatttttta tcggaggcaa aatccaatcg ggctgattcc
    16921 gaagttggga aatgaatgga agcctttccc ctgggctcgt cgtccgggtt tatattgaga
    16981 ggaaagattt ccttagcttt ctccggccca atcttttcca ccagcatctg agcaatgatc
    17041 tcggtttcga tattggctgc cgattcccag ctcatgagat aaaaaaccgc catagattct
    17101 gccaattccc aaggtttagg cttgatgcct gccagtttgt attccagggg atattctcct
    17161 gagcgggttt gaatgaaggc gttgaccccg tcaatatact tctgcatgag gttcttcgtc
    17221 tccgggtcca tgatggatac atggcgcttg gcatggcggt ggaaaccgat cgtccgcatg
    17281 cgggtatcaa tggcctcgcc tttctccccg atcatttcgc tgattcttcc gctagcgaag
    17341 aggcggatca attccatctg gaagagccgg tcctgggctg tggtgaagcc aaaagccacc
    17401 atggcatctt ccggactctt ggcgtagatg taggccattc ctttttcatc ccgcaccacg
    17461 gtcaccggtt ctttcaaccc cggaagagtc ccttccccct cagcctgata tcggttcagg
    17521 cccaaatggg tgcaggaaga gagcgctaag gaagccagca agaaaagagc aaccaggaac
    17581 catgtttttg aggccttcat cgtcaccctc ccgatgtttg aattggtgct attttacgat
    17641 gctggataaa tttagcaaca gaaaagccct gtgccattga gagaaattcc tattggaaaa
    17701 caaggaaaat tctgtataat catcggcgaa agccgggtct cagagtcttt attcttcaat
    17761 ttttcccttc aggaggagac catggagata aaaggtaaga cggcattggt taccggagga
    17821 gcttcgggtt tgggggcggc tgccgtcaga aggatcgtag cagccggcgg aaatgcggtg
    17881 atcctcgatg tgcaggagga gaagggaaaa actctagcct ccgagctggg gaagacagcc
    17941 tttttttgcc ggaccgatgt gaccaacgaa gaaagcgcga atcagactat tgcaaaagcc
    18001 aaggagaatt atggaggcgt tcacttctgc gtaaatgctg caggaaccgg gtgggcggag
    18061 cgcacggtta cgaagaccgg gccccatact ctgtccaatt ttgagaagat tatccgcctg
    18121 aatctcatcg gaaccttcaa tgttgccagc aaggctgctt ttgccatgag caccaatcct
    18181 ccggatgcaa acggcgagag gggggttatc gtcaacgtat ccagcactgc agcctttgac
    18241 ggccagatcg ggcaggccgc ttattcggct tccaaggggg cggttgtggg gatgaccctc
    18301 cccatggccc gtgacctgtc cagtttggga atccgagttg tcaccatagc ccccggtctt
    18361 ttcaacaccc ctcttcttgg actcttgccg caggattata ttaaagctct gaacgagtcc
    18421 gtgccttttc ccaagaggtt gggagacccg gaagaattcg ccctgctggt ggaacagatc
    18481 atcctgaacc catatttgaa cggagaagtg atccggctgg atggcgccct tcgcatggca
    18541 ccgaagtagt ctaagtgata gtgtcagtgg aaaagcaacg aaaagaacct aaaatgggtc
    18601 gcggatgaaa atggttaagg gcggatgttt ttggaaaaga cgggaaaatc ctaaagaaga
    18661 gggggtttga gggaggatga gcggagagaa ctcaaaggcc attgcccagg atctgatccg
    18721 gagggtgctg gaaaattctc ccttcttccg gcatctccag ctggaatttc tggaggccgc
    18781 cgaaggcatc ggacggttgg ctttgaaggt ccagcctttt cacctgaata cccaggggat
    18841 cgttcaagga ggcgtcttat catccctagc ggatacagcc gcattgctgg ccatcgggac
    18901 caagctctcc ctagggcaga ggccgcggac cgttcagatg gacatccatt ttctggcacc
    18961 ggcgcagaag ggaaccttga ccgccgtcgg gcgcatcgct aagatgggga gcttggtctc
    19021 ggtgagcgat gtggagattc aggacgagac cgggaaaacg gtctgtatgg ctaggtgcac
    19081 gagtgtgcta gtcaatgttg cgggagacga aaaattcata ccgaaaatag agtaggcgcg
    19141 attcatgaat cgcccctaca agatttccaa atatggccac tgcaaaggcg gttcgcgaac
    19201 cgcctcctag ctcaattttt attcagaatc attcgcacat ccagggccat gcagccttcc
    19261 cggatggaga agacccgcag ggggttgatg tccatctccc ggatttcagg gaaatcgctg
    19321 agaagctggg agagccgcaa aagaacatcc accaccgatt ggatgtcaga tggttttaat
    19381 ccccgggctc ccttgaagat gggagcgccg cgcagctcat cgatcatctc catcgcttcc
    19441 cggtgggaga tgggggctac gcggacagaa acttcgccaa agacctccac aaaaatcccc
    19501 cctaacccga caaggacaac ggaaccgaac tgttcatcct gccgtccccc gacgatcagt
    19561 tcccttcccc cggtgaccat agactggatg aggacccctt cgattttagc ctgaggatat
    19621 tcctggcgga tccggcggag catatcctgg taagcgtcgg ctactcccgc ctcgctcctc
    19681 aggttcaatt gcacaccccc cacgtcggac ttgtgggaga tctcctggga gatcaccttc
    19741 atagccacgg ggaagcccaa ctgcctagcc gcttgaaccg cttctttctc gtcagaaaca
    19801 ggcactcccc ggaccatggg gattccgtag accctcagaa cttcggccgc ttcgtggata
    19861 aagaggtctc gattctcctt ccgggccttt tgcagcaagg gcctcacttt ctctttgtcc
    19921 gccgggaagg aaggaatctc cgcccgctgg cgcaagcgct ggctttcggc ataatggcgg
    19981 cgattgattc ccagggcccg aaccgtctcc accacttggg tgaagatggg atagttgaag
    20041 ttgcgcttca ggtagttcac ttcctggtct tccgtggaca cgtagatggc cacgggtttg
    20101 tcatactttt gggaaagctc catgaccttc tggaaaagct ccctggaccg cggaccttca
    20161 aaggtggcat tgaaggtgtg gaggaaaacg atcccatcca cctccttctg ggccagggtc
    20221 cgatcgatga tcttcaagta gatttccaga tcg
//
LOCUS       DASPRR010000458         5737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2202805, whole genome shotgun sequence.
ACCESSION   DASPRR010000458 DASPRR010000000
VERSION     DASPRR010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202805"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VEL68_16815"
     CDS             <1..1202
                     /locus_tag="VEL68_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ37687.1"
                     /translation="LWGAKGLFVAAAQAYGAMGKFTLLAIPLFVFMAMILERAGVAEN
                     LYTMMHRWMGPVRGGLAIGTVIICAIFAAMSGISATATVSMGIIALPAMLKRGYDKIL
                     AMGIISGGGALGILIPPSVPMILYATLTGESIGGLFAGGIFPGILLTLIFILYIGIRC
                     FFQPHMGPALPKEERATWEEKFVSLRAVALPIGIIIMVLGSIYTGVCTATEAAALGVL
                     GAFISAAVYGKLKRELIKEACYRTASLTAMIVWILIGAYCFTSVYTGTGAHELMEKLL
                     LSIPGGRWAIMGTIQVVFFILGCLLDPAGIIMICTPVFVPVIKSLGFDPLWFGVLFIM
                     NMEMGYLTPPFGFNLFYMKAIAPPGVTMGDIYRSIIPIVGLQAVALIIVMIFPEIALW
                     FPRSLTR"
     gene            complement(1314..3218)
                     /gene="dxs"
                     /locus_tag="VEL68_16820"
     CDS             complement(1314..3218)
                     /gene="dxs"
                     /locus_tag="VEL68_16820"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXZ37688.1"
                     /translation="MGDLLKQIQNPSDLRRLPSADLTPLAEEIREEIVQAVSKVGGHL
                     ASSLGVVELTLALHYVFDTPRDRIVWDVGHQTYAHKLITGRRENFRTLRQSGGICGFP
                     RREESPYDAFGTGHSGTSISAALGMAQARCLRGEDFKVIAVIGDGSMTAGEAFEGLNQ
                     AGAMEKDLIVILNDNELSISPNVGALSSYLNRLMTGQFATHFREEIKSFLRKIPGIGS
                     SALKWARYAEESLKGFFLPGLLFEELGFKYVGPLPGHKIDSLIENFRNIKQLPGPILV
                     HVLTTKGKGYPPAEKDPVMFHGVGPFIAETGEIRKAKGGCLSYTEAFGQTMVKLARQI
                     PCLVGITAAMPQGTGLEGFAAEFPDRFFDVGIAEQHGITFAAGLAVEGFIPVVAIYST
                     FMQRAYDQVLHDVCLQNLPVVLALDRGGIVGADGSTHQGLFDFSYLRHIPNLVVMAPK
                     DENELQHMLKTAISCGQPASLRYPRGNGWRGFLDEEAKELPVGKGEVLRQGKDLILLA
                     IGSTVKAALQAASRLQSRGVEASVVNARFLKPLDSELICSLAVDCPRLISVEENALQG
                     GFGSAVLELLEEKGMTGVEVKRLGIPDVFVEHGAQEFLRHKYGIDAEAIVQAAEALLK
                     VRNVECGMRS"
     gene            complement(3270..4160)
                     /locus_tag="VEL68_16825"
     CDS             complement(3270..4160)
                     /locus_tag="VEL68_16825"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXZ37689.1"
                     /translation="MKLADYLQRRQHWVEEALKRWIPGENEFPPPIHQAMRYSLFAGG
                     KRLRPILVLAATEAVGGSIREALPLACSFELIHTYSLIHDDLPSMDNDDMRRGKPTSH
                     KVFGEALAILAGDALLTEAFHLATRPEVMKKVPARRRIQTLWHLARAAGSLGMVGGQT
                     VDILTQGQPVDTRTLEYIHAHKTGALIAASVAVGAIVGGATARQFKSLQGYGEKLGLA
                     FQIIDDLLDEVGEEARLGKAVGKDQTKGKATYPGLYGIPESRRQAEELIAGALHHLQS
                     FDRRANPLREIARFILNRKN"
     gene            complement(4162..4398)
                     /gene="xseB"
                     /locus_tag="VEL68_16830"
     CDS             complement(4162..4398)
                     /gene="xseB"
                     /locus_tag="VEL68_16830"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXZ37690.1"
                     /translation="MAEQKFEDAFQKLESIVKKLEDGNLSLEESLKAFEEGVRLSRFC
                     SKKLDEAEKKVEVLLKDSDGRLLPKPFSLEEEGD"
     gene            complement(4490..>5737)
                     /gene="xseA"
                     /locus_tag="VEL68_16835"
     CDS             complement(4490..>5737)
                     /gene="xseA"
                     /locus_tag="VEL68_16835"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXZ37691.1"
                     /translation="YFTLKDESCQLRAVMFRMQNRLLKFDPEDGLQIVCYGRLTVYDP
                     RGEYQIVVDYMEPKGLGALQLAFEQLKEKLAREGLFDSARKRPLPTLPQKIGIVTSPT
                     GAAIRDILQIIDRRFANVHILLYPVRVQGTGAAQEIAQAIEELGRWPALEVMIVGRGG
                     GSLEDLWAFNEEEVARAIHRSPVPVISAVGHEIDFTIADFVADLRAPTPSAAAELVVR
                     NKVELVQNLENRVWRLNQVVRTTLESWQERLESLIHRLTDPRKRIADQRLRLDDFSFR
                     LATSIQQGLGRRSERLGLKRESLFLLHPGKRVADFSRRLSQAYHRLTISGRVALRLNR
                     QRVEVIAGRLRTLSPLSVLERGYSIVRRLPSQEIIREASRLKPHDRVNVKVHRGEFTA
                     RVEKVAGGEEDPQPSLTLGSKGV"
BASE COUNT         1160 a         1689 c         1652 g         1236 t
ORIGIN      
        1 ttctctgggg ggccaagggg cttttcgtgg cggccgccca ggcctacggg gcgatgggga
       61 agttcaccct tctggcgatc ccgctgttcg tcttcatggc catgatcctg gaacgtgcag
      121 gggtggcgga gaatctctac accatgatgc accgctggat gggaccggtc cggggcggtc
      181 tggccattgg cacggtgatc atctgcgcca tctttgcggc catgtcgggg atcagcgcca
      241 cggccacagt atccatgggt atcatcgccc ttccggccat gcttaagcgg gggtacgata
      301 agattctggc catggggatc atctccggcg ggggagcgct ggggatcctc atccctccca
      361 gcgttcccat gattctctac gcaaccctca ccggggaatc catcggcggg ttgtttgccg
      421 gtgggatctt cccggggatt ctcctgaccc tgattttcat cctttatatc ggaatccgtt
      481 gtttcttcca gccccacatg gggccggccc tgccaaaaga ggagagggct acctgggagg
      541 agaaattcgt ctccctaagg gccgtggccc ttcccatcgg gatcatcatt atggtcctgg
      601 ggtccattta tacgggggtc tgcacggcca cggaggcggc cgccctgggc gtcctggggg
      661 cgttcatatc cgcggccgtt tatggaaagc tgaaacggga actcatcaag gaggcctgct
      721 accgcacggc gagcctcacg gcaatgatcg tctggattct catcggggcc tactgcttta
      781 catccgtgta cactggcacc ggcgcccatg aattgatgga gaaactcctg ctttccattc
      841 ccggggggcg ctgggccatc atggggacga ttcaggtggt cttcttcatc ctgggatgcc
      901 tgctggaccc ggcggggatc atcatgatct gcacaccagt ctttgtcccg gtgatcaagt
      961 ccctgggctt cgaccccctt tggttcgggg tgctcttcat catgaacatg gagatgggct
     1021 acctcacccc gcccttcggt ttcaacctct tctacatgaa ggccatcgct cctccgggcg
     1081 tgaccatggg ggacatctac cggtcgatca ttcccatcgt gggccttcag gctgtggctc
     1141 tgatcattgt gatgatcttt ccggaaatcg ccctctggtt tccaagaagc ctgacccggt
     1201 gagagaggtc aaggtttaat gaaggcgttg ctggttgctg gtttacaaaa gacggcaagc
     1261 tccccaggga aaagataaca aggaactagg aacgagcaac caggaaccgc tttttaactc
     1321 cgcattccgc attccacatt ccgcactttg aggagcgctt ccgccgcctg cacgatggct
     1381 tccgcgtcga ttccgtattt gtgccgcaaa aattcctggg ccccgtgttc cacaaagaca
     1441 tccggaatcc ccaagcgctt cacctccacc ccagtcatgc ccttctcttc caggagttcc
     1501 aggaccgcgc tgccgaatcc tccctggagc gcattttctt caacgctgat cagtcgcggg
     1561 cagtcaacgg ccaatgagca gatcaactca ctgtccaagg gtttcaggaa gcgggcatta
     1621 accaccgaag cttcaacccc ccgcgattgg agccgggaag cggcctggag ggcagccttc
     1681 accgtggagc cgatggccaa aagaatcagg tccttcccct gcctcaagac ttcccccttc
     1741 ccgaccggga gttctttggc ctcttcatcc aggaatccgc gccagccgtt tccccgggga
     1801 tagcgcaggg aggccggctg gccgcaggag atcgccgtct tgagcatgtg ttggagttca
     1861 ttttcgtcct tgggggccat gaccaccagg ttgggaatgt ggcggaggta ggagaaatcg
     1921 aagaggcctt gatgggtcga gccgtcggcg ccgacgatgc ccccccggtc cagggccaga
     1981 accacgggca ggttctgaag gcagacatcg tgcaggacct gatcgtaggc ccgctgcatg
     2041 aaagttgagt aaatggccac cacagggatg aacccttcca ccgcaaggcc ggcggcaaag
     2101 gtgatgccat gctgttcggc aattcccacg tcgaagaagc ggtcggggaa ctcggcggca
     2161 aaaccttcca accctgttcc ctggggcatg gccgcggtaa tgcccaccag acaggggatc
     2221 tgccgcgcca gtttaaccat ggtctgcccg aaagcctcgg tataagagag acagcctcct
     2281 tttgctttgc ggatctcccc cgtttcagcg ataaagggcc cgaccccgtg gaacatcact
     2341 gggtccttct ctgcaggggg gtaacctttc cccttggtgg tgagcacatg aacgaggatg
     2401 gggccgggaa gctgcttgat gttgcggaag ttctcgatga gagaatcgat tttgtgcccg
     2461 ggcaagggtc cgacatactt gaatcccagt tcctcgaaga gcagaccggg caggaaaaag
     2521 cccttgaggg actcctcggc atacctggcc catttcaagg cggagctccc gatgccgggg
     2581 atctttctca aaaaactctt gatttcttcc cgaaaatggg tggcgaactg cccggtcatc
     2641 agccggttaa ggtatgaaga aagggctccc acattggggg agatggagag ttcgttatca
     2701 tttaaaataa cgatgaggtc cttttccatt gctccagcct ggttcaaccc ttcgaacgcc
     2761 tctcctgcgg tcatggaacc gtctccaatg acggcgatga ccttgaaatc ctcgccccgc
     2821 aagcagcggg cttgagccat tcccaaggcg gcagagatgg aggtgccgga atggccggtc
     2881 ccgaaagcgt cgtaagggct ctcctcccgt cgcgggaacc cacagatccc tccgctctgg
     2941 cgaagggtac gaaagttctc ccggcgcccg gtgatcagct tatgggcata ggtctggtgg
     3001 cctacgtccc aaacgattcg gtcgcggggg gtatcgaaga cataatgaag ggccaaagtc
     3061 aactctacca ctcccaggct ggaagccagg tgccccccca ccttggagac ggcctgaacg
     3121 atctcctccc ggatctcctc agccagcggg gtcaaatcgg ctgaaggcag ccggcgtagg
     3181 tctgaaggat tctggatttg tttgagtaag tctcccatgt cagtaaagaa ggaatagcga
     3241 aaagagcata gcccgtagcg caaaaacaat caattcttgc gatttaaaat gaacctggcg
     3301 atctccctca gcggattcgc ccgccggtcg aaggattgta gatgatggag cgcccccgca
     3361 atgagctctt ctgcctgccg ccgggattct ggaattccgt ataagcctgg atacgtggcc
     3421 ttccctttgg tttgatcttt gccgactgcc ttacccagtc ttgcttcctc ccccacctca
     3481 tccagaaggt catcgatgat ctgaaaagcc agtcccaact tttccccgta tccctgaagg
     3541 ctcttgaact ggcgtgctgt ggctccgccc acaatggccc ccacagccac ggaagcggca
     3601 atcagcgcac cggttttgtg ggcgtgaatg tactccagag tgcgggtatc caccggttgg
     3661 ccctgggtca ggatgtccac ggtttgaccc ccaaccatgc cgagggagcc ggctgcgcgg
     3721 gccagatgcc acagggtttg aattctcctg cgagccggaa cttttttcat cacctcgggc
     3781 cgggttgcca gatgaaaggc ttcggtcagc agggcatccc cggcaagaat ggccaaggcc
     3841 tcgccgaaaa ccttgtggct agtcggtttc cccctgcgca tatcgtcgtt atccatggag
     3901 gggaggtcat cgtggatcag ggaataggtg tggatgagtt cgaaagagca ggccagggga
     3961 agagcttccc ggatcgatcc tcccaccgct tcggtggcag ccaaaaccag gatgggccgg
     4021 agccgttttc ccccggcaaa gagactgtat cgcatggcct ggtgaattgg ggggggaaac
     4081 tcgttttcac ccggaatcca gcgtttcagc gcctcctcga cccaatgttg ccggcgctga
     4141 aggtaatctg ccagcttcaa ttcaatcccc ttcttcttcc aaggagaagg gtttgggaag
     4201 cagacgcccg tcgctgtctt tgaggaggac ttccactttc ttttcggctt catccagctt
     4261 cttggaacag aagcgggaca ggcggacccc ttcctcaaat gccttaaggg attcttccag
     4321 gcttaaattt ccgtcttcca gctttttgac tatggactcc aatttctgga aagcgtcctc
     4381 gaatttctgc tcggccacca aaccccctca caattgcgga ttgccgattt cggattgcga
     4441 atttaagaaa tagaaatccg caatctgcaa tccgaaattc gcaatattat tatactccct
     4501 tcgaccctag ggtcaaggag ggctgggggt cttcttcacc cccggcgacc ttttccaccc
     4561 gggccgtgaa ttctccccta tggactttga cattcacccg gtcatggggc ttcaagcggg
     4621 aagcctcgcg gatgatctcc tgtgagggca acctccggac gatactgtac ccccgctcca
     4681 gaacggacag aggactcagg gtccgaagcc tcccggcgat aacctcgact cgctgtcggt
     4741 taaggcgcag agccactcgg ccggatatcg tcagccgatg ataggcctgg ctgagacgcc
     4801 gggaaaagtc ggccacccgc tttccagggt gaaggagaaa aagactctcg cgcttcaatc
     4861 ctaagcgttc agaccgccgc ccgaggccct gctgtataga agttgccagg cggaaggaaa
     4921 aatcatccag gcgcagccgc tggtcggcga tccgttttcg ggggtcggtt aagcggtgga
     4981 tcagcgattc caggcgttcc tgccaagact ccaaggtcgt ccggaccacc tggttcaggc
     5041 gccagacccg gttctccagg ttttgaacca gttccacttt atttcggacc accagttcgg
     5101 ctgcggccga aggggtaggg gcccgcaggt cggcgacgaa atcggcgatc gtgaaatcaa
     5161 tctcatggcc cacggcggag attaccggca caggagaacg atggatcgcc cgggccacct
     5221 cttcttcgtt gaaagcccat aggtcttcca gggagccacc ccccctccca acgatcatga
     5281 cctccagggc gggccatcgg ccgagttctt cgatggcttg agcgatttct tgggcagccc
     5341 ccgtcccctg gacgcggacc ggatagagaa ggatatgaac gttggcgaaa cggcggtcga
     5401 tgatctggag gatgtctcga atggctgcgc ccgtggggga ggtgacgatg ccgatcttct
     5461 ggggaagggt tggaaggggc cttttgcggg ccgaatcaaa aagcccttcc cgggcaagct
     5521 tctctttcag ttgctcgaag gccaactgga gggctcccaa gcccttgggt tccatgtagt
     5581 cgacgacgat ctgatactcg ccccggggat cataaacggt cagccttcca tagcatacaa
     5641 tctggaggcc gtcttccgga tcgaatttca acagccggtt ctgcatccgg aacatgaccg
     5701 ctcgcagctg gcatgactca tccttcaggg tgaagta
//
LOCUS       DASPRR010000459         5559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1559122, whole genome shotgun sequence.
ACCESSION   DASPRR010000459 DASPRR010000000
VERSION     DASPRR010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559122"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VEL68_16840"
     CDS             complement(<1..417)
                     /locus_tag="VEL68_16840"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ37692.1"
                     /translation="MMKEVSLFIPCTVDLFAPRIGEAVVHILRRVGVNPIYHEEQTCC
                     GQPAMNAGYREEAKKVAKHFISVFEKDPVIVSPAGSCVYMVKDHYLEILEDEPDWFRR
                     AKDLAPRVYEFSQYLVDVLKVEDVGASFEGKVTYHES"
     gene            689..1660
                     /locus_tag="VEL68_16845"
     CDS             689..1660
                     /locus_tag="VEL68_16845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37693.1"
                     /translation="MKRFIWALLILASAWALGGLSWAAEKYPNKPITFLVPWGAGGMT
                     DVTSRLLAEKFKAELGQPVLVVNKPGANGAIGLKYALTQKADGYTVVIGPMTDGFAGP
                     YFLGGEPFDFKDFSFVAGYMPQQRVLFTTADKPYKSFQEFIDYAKKHPGEISAGSGGA
                     QWALEVVKSIAVKNDLKMKYVMFKSGGEASTAILGKHVDLCETGTGTPAFQAARDGKL
                     IMLVNLGSGSVAYFPKVKNMEELGYPFASIVEYGFTVRAGTPEEVRKKLEDTLNKVVN
                     DPEVKSKLESMGLTPRFIDGKQYGEIVMKAVKSVPELIQYSKKAQEG"
     gene            complement(1978..3513)
                     /locus_tag="VEL68_16850"
     CDS             complement(1978..3513)
                     /locus_tag="VEL68_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ37694.1"
                     /translation="MLEIFQSLLHGFSLALSPLYLYYALIGAILGTIVGVLPGLGPLG
                     AMAILLSFTLKLDATGAMILFAGIYYGAMYGGSTTSILLNIPGEAASVVTCIDGYQMA
                     QKGRAGAALTVSAVGSFIAGTISILGLMGVALFLADIALRFGPPEFFAIGVCGLALLV
                     RLSEGSPLQAVIMILLGMALATVGVDFLTGIERFTFGIQELGQGIDFLPVAMGIFGIA
                     EVLTTVEQKEFIRPAQKVKLRELLPTRQEWKRSFPPMFRGSFLGFIIGLVPGPSPVIA
                     TFVSYITEKRLSPTPEDFGKGAIEGVAGPESANNAAVGGAYVPLFALGIPFTPAMAIV
                     LGTLMLHGLTPGPNLIQTKPDLFWTVIASMYIGNFMLLVLNLPLVNVFASILKVPQNI
                     LLPVIVLICLIGVYTVNSSSLDLLMLAVFGFVGYALRGVGFHAAPLILAMVIGPMLET
                     SLRQSLNITGGNLSGMLLRPICLAMYIIVALTFVLPFVRKCIRRSTNKSQSPDKQTGN
                     SAP"
     gene            complement(3520..4017)
                     /locus_tag="VEL68_16855"
     CDS             complement(3520..4017)
                     /locus_tag="VEL68_16855"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ37695.1"
                     /translation="MEIHASRNQKITGFILFGFSLFYFFSSLRLKLGTFKNPGPGLIP
                     VAIGGFLLLCTGIYLIRVLRLKPPGEGNLAPSRRGPKNYPAIFGILGCTLAYPFLLEY
                     GKFILSTFAIAYLMLAFLKPSRTLFPFFLALGMAVGSFLVFSRFLGVALPSGFLEDFL
                     FRIGG"
     gene            complement(4431..>5559)
                     /locus_tag="VEL68_16860"
     CDS             complement(4431..>5559)
                     /locus_tag="VEL68_16860"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ37696.1"
                     /translation="NTGALPAFLPFIKDSLGLSYTLTASIILVFNVTSSVIQPVFGYL
                     SDRWSIRWLLPVGPFLASLGLGLLGLGPSYAWILLFAAVSGLGQASYHPEGFKTVSFL
                     SGTKKATIFSIFHFGGNLGFAVGPILATFFFVHIGLKGSLLFIFPGVVITAIFLLFPY
                     WNVKTSPSAHEAAPGPKTSLTQPNHTPMLLLILVVVLRSATRLGLMTFVPFYFIHLLN
                     RDPMVAGQYLSTFLFAGTVGILAGGPLADRYGYKKMVLITLGLSSIFLYLFYATQGTL
                     SLVFFTLAGLVLISSNSTTMAMAQSFMPRNLGMASGLILGFSMGIGGVSTTILGWVAD
                     HWGVPSALQITFVLPFLAFLACWPISYPPKPMRNADCGLRN"
BASE COUNT         1445 a         1414 c         1619 g         1081 t
ORIGIN      
        1 ggattcgtga taggtcacct ttccttcgaa ggaagccccc acgtcctcca ccttcagcac
       61 atccaccagg tactgggaga attcatacac ccgcggagca aggtccttgg cccgtcgaaa
      121 ccagtccggt tcgtcttcca aaatctcgag gtagtggtct ttcaccatat agacgcagga
      181 gcctgcggga ctcacgatca ccgggtcttt ctcaaagacg gagataaaat gcttggccac
      241 ctttttggcc tcttcccggt acccagcgtt catcgccggc tggccgcaac aggtctgctc
      301 ttcgtggtag atcggattca cccccacgcg gcggaggata tgtactaccg cctccccaat
      361 tcggggggcg aagaggtcga ccgtgcaggg aatgaaaaga gacacttctt tcatcatcga
      421 ctcctttcgt cttaatccgg agcgaatcaa ggggaaaacc atttagaaaa aaagaaagcg
      481 agccaccagg cccaccccta tttttagcag aatgaaaaga aggtggtcaa gggaaaagcg
      541 gcatgataaa atccccaaca atcagggcac cattaggccg agggaaaggg ttgaggtacc
      601 tcccgggaat ctgcgaccgt cttaccctag cgcgcggcag gagctcggta atcaatcatt
      661 ttctgcactc atttcaagga ggtttcctat gaaaagattt atttgggcct tgttgatttt
      721 ggcgtcggcc tgggctttag ggggattgtc gtgggccgct gaaaagtacc ccaataaacc
      781 cataaccttc ctggttcctt ggggagcagg aggcatgacc gatgtgacct cacggctgtt
      841 ggcggaaaaa ttcaaagcag aattggggca gcctgtgctg gtggttaaca aaccgggggc
      901 gaacggtgcc atcggcctga aatacgccct gacccagaag gctgatgggt ataccgtggt
      961 gatcggcccc atgaccgatg gcttcgcagg accttatttc ctgggcgggg agcccttcga
     1021 tttcaaggat ttttcctttg ttgccggcta catgcctcag cagagggttc ttttcacgac
     1081 agcggataag ccctataaat ccttccaaga gttcatcgat tatgccaaga aacaccccgg
     1141 tgagatatct gcggggagcg ggggcgccca gtgggccctg gaggtcgtga aatccatcgc
     1201 cgtgaagaac gatttgaaga tgaaatacgt gatgttcaaa agcggcgggg aagcttccac
     1261 ggccatcctg ggcaaacatg tggacctatg cgagacggga accggcacac cggcttttca
     1321 ggcggcccgc gatggcaaac tcatcatgct ggtaaatctg gggtctgggt cggtcgctta
     1381 tttccccaag gtcaagaata tggaagagct tgggtatccg tttgcctcca tcgttgaata
     1441 tgggtttacg gtgcgcgcgg gcacgccgga agaggtccgc aaaaagttag aggacaccct
     1501 caataaggtg gtgaatgacc ccgaagtcaa gtcaaaactc gaatccatgg gattgacccc
     1561 ccgattcatc gacgggaaac aatacgggga aatcgtgatg aaggcggtga aatcggttcc
     1621 ggagttgatc caatacagca aaaaggcaca ggaaggatag gtacggcata ttcggaatgc
     1681 ggagtgcgga atgcagaatt attaaggagt ccataattga gaaaacatgt tcttctgaaa
     1741 atctcctcta attccgatgt tctttacgta agctgtcacc gttcaccctg agcttgtcga
     1801 agggtgcgtc gctacgccgt tcatgcttcg acaagctcag cacgaacggc ttatttttca
     1861 gcgtccatac agtattggac ggattagtaa aatacaagca ccaaacccaa gcaggaatcc
     1921 taggaagcgc gggaacaaat aaatcccaat gaccaaaatc cagaatttcc aataccttta
     1981 gggggctgaa tttccggttt gtttatctgg ggattgggat ttattcgtgg accggcggat
     2041 gcatttccgt acaaagggaa gcacgaaggt cagggccaca ataatgtaca tggccagaca
     2101 gatcggccgc agaagcatcc cgctgagatt gcctccggta atattcaggg attgtcgcaa
     2161 agaagtttcg agcatgggac cgatcaccat ggccaggatc aggggggctg catgaaaccc
     2221 caccccccgc aaggcataac cgacgaagcc aaagaccgcc agcatgagaa ggtccaggga
     2281 actggaattc accgtataga ccccgatgag gcagatcagg acaatgaccg gcaggagaat
     2341 attctggggc accttcagaa tactcgcgaa tacattgacc aggggaaggt tcaacaccag
     2401 gagcataaaa ttcccgatgt acatgctggc gataaccgtc cagaaaagat cgggcttggt
     2461 ttgaatcagg ttcggccccg gagttagccc gtgaagcatc aatgttccca gaacgatggc
     2521 catggccggc gtaaagggga tccctagggc aaaaagagga acgtaagcgc cgcccacggc
     2581 agcgttgttc gcagattccg ggccggccac cccctcgatg gccccctttc cgaaatcctc
     2641 cggagtgggg gagagccttt tttcggtaat gtaagagacg aacgtggcaa tcaccggtga
     2701 aggcccagga accaggccga taatgaagcc caaaaaggaa cctcgaaaca tgggagggaa
     2761 ggatcgtttc cattcctgcc gggtgggcag gagttccctc agcttcactt tctgggcggg
     2821 ccggatgaat tccttctgct ccaccgtggt gagaacctcg gcaattccga agatccccat
     2881 ggccacgggc aaaaaatcga tcccctgtcc cagttcctga atgccgaagg tgaatcgttc
     2941 gatcccggtc aggaaatcta ctcccaccgt ggccagggcc attccgagaa ggatcatgat
     3001 gaccgcttga agaggggacc cttcgctcaa tcgcaccagg agagccagcc cgcaaacccc
     3061 gatggcaaag aattccggag gcccaaaccg cagggcaata tccgccagga agagggccac
     3121 tcccattaaa cccaggatgc tgatggttcc ggctatgaag gaccctaccg ccgagacggt
     3181 cagggctgcc ccggcccgtc ctttctgggc catctggtac ccatcgatgc aggtcactac
     3241 agaggcggct tccccgggta tgttgaggag gatggaggtg gtggagccgc cgtacatagc
     3301 gccgtaatag atgccggcaa aaaggatcat ggccccggtg gcatccagct ttagggtgaa
     3361 agaaagaagg atggccatgg cccccaaggg ccccagcccg ggaagaacgc ccacgatcgt
     3421 tcccaggatg gctccgatca gggcataata cagataaagg gggctcaggg cgaggctgaa
     3481 accatggaga agactctgga agatttctag catggggagt tatcctccga tccgaaagag
     3541 gaaatcttcc aggaaaccgc tgggcagagc cactcccaaa aagcgggaga agaccagaaa
     3601 ggaccccacc gccattccca gagcaaggaa aaaaggaaaa agggttctgg agggtttcag
     3661 aaatgccagc atgaggtaag cgatcgcaaa ggtggagagg atgaattttc cgtattccag
     3721 gaggaacggg taggccagcg tgcaacccag gatgccgaag atggccgggt aattctttgg
     3781 ccccctccgg ctcggtgcta ggtttccttc cccagggggt ttcaaccgca aaacgcggat
     3841 cagataaata cccgtgcaga gcagcaaaaa accgccgatc gccacgggga tcaaccccgg
     3901 tcccgggttc ttgaaggtcc ccagttttag gcgcaaagag ctgaaaaaat aaaaaaggga
     3961 aaatccgaaa agaatgaagc cggttatctt ttgatttctc gaagcatgga tttccatcgg
     4021 ctcaaccctt cgcccgtttt gggcgtcgct gcaagtcgga aaatatcatt ctttgaccga
     4081 ttgcgcaatg cgccggggat cattttccat tttccatcgg cccctaagct cgctgtcagg
     4141 gcgggggggt ggccaagccc agggggtgcg tcccgtccat ggcgggacat aatgaggcac
     4201 gaattctttc atcggttctg gtcggaaaat acttgggtta ccaggttcct gagccgggat
     4261 tcggttttgc gagagggagt cggggacagg tgctttacgg aatctaagca accgcttcga
     4321 aatctggttt tttcccgccg attctgcccc gccatgggcg ggacgccccc cctgggttgg
     4381 gaatattttc tgttacctct ccacccactc gatcgggata agacccattt ttaattccgc
     4441 aatccgcaat ccgcattccg cattggttta ggcgggtagg agatgggcca gcaggccagg
     4501 aaagcaagga aagggaggac aaaagtgatc tgcagggcgg agggaacgcc ccagtggtca
     4561 gcaacccaac ccaggatcgt ggtggaaacc cctccgatcc ccatggagaa tcccaggatt
     4621 agcccggagg ccattcccag gttccggggc atgaaactct gggccatggc catggttgtg
     4681 gagttggagg agatgagtac cagcccggcg agggtgaaga agaccagcga gagggtcccc
     4741 tgggtggcat agaaaaggta gagaaagatc gaactcaatc ctagggtgat gagaaccatc
     4801 tttttatagc catatcggtc agcgagagga cccccggcca ggatgcccac cgttccggcg
     4861 aaaagaaaag tagagaggta ttgcccggcg accatcgggt cccggttaag aagatggatg
     4921 aagtagaagg ggacgaaagt catcaaaccc agccgggtcg cggaccgtag aaccacgacc
     4981 aagattaaca gcaacatggg ggtatggttt ggctgtgtca aacttgtttt tgggccggga
     5041 gcggcctcat gggcggaagg actcgtcttg acattccaat acggaaagag aagaaagatg
     5101 gcggttatga ccactccggg gaagatgaac agaagagaac ccttgaggcc gatgtggaca
     5161 aagaaaaagg tcgccagaat gggccccacc gcaaatccca ggttgccacc gaaatgaaag
     5221 atggaaaaaa tggtcgcctt tttcgttccg cttaaaaagc ttacggtctt gaacccttcg
     5281 ggatggtaac tggcctggcc aaggccgctt acggcggcaa agagcaaaat ccacgcgtaa
     5341 gaaggcccca ggcccaggag ccccaagccc agggaggcga gaaaggggcc tacgggaagg
     5401 agccatcgaa tggaccagcg gtcggaaagg taaccgaata cgggctgaat gaccgatgaa
     5461 gtcacattaa aaaccagaat gatgctggcg gtcagggtgt aagagaggcc gagggaatct
     5521 ttgatgaaag ggagaaaggc gggaagggcg ccggtgttg
//
LOCUS       DASPRR010000460         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1559553, whole genome shotgun sequence.
ACCESSION   DASPRR010000460 DASPRR010000000
VERSION     DASPRR010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559553"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..322
                     /locus_tag="VEL68_16865"
     CDS             137..322
                     /locus_tag="VEL68_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37697.1"
                     /translation="MRKDLIAKIALSKEEIAHLYQGGVMSIKIKGKDTNASVEISCRE
                     VSERKGTRIRFKTIDAE"
     gene            552..2159
                     /locus_tag="VEL68_16870"
     CDS             552..2159
                     /locus_tag="VEL68_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37698.1"
                     /translation="MRKAFLVMAVILLLGFMGGEAAAARSGGTFNFCVPYGGDVLTLD
                     PHKGARVNDMIVALSLHRSLYGWDPENNRPKLDLGEKADVSPDGLVYRIYLKKNVKFH
                     NGRGMTADDVIWSYERIMSPKTASLSAGFVRVIKGAKDYEDGKADKISGLRKIDNFTL
                     EITMENPVDPAYTLYEIGTGILPREEVEKKGFDFGSEPVGCGPFKFVKWVKGSEIVLT
                     RNPDYYEKGKPYLDKLVYKIMPEGASRDLAFRAKELDATVVGSTQYPAYKADPQISKN
                     MVEVAELFTRIIGFNPKFEPFAKKQVRQAINYAIDSRLIIQKLLKEKAFPCVSYLPST
                     SPAFDPQAKGYEFNLPKAKALMKEAGYEKGFTFECIGMNNEAWGVVIVEAIMPFLKQI
                     NVTVKPQQLEGAALADRVRKMDYQGFIWSVASGAGGDPLQALNRWSSKNPPTAGNFVA
                     YNNPEFDKLLDAASQIRDVSKRIDLLRKADAIFREDAPVWFFNYNKAVVAHQPWVHGI
                     QTVAIEMMYQNMADIWVEESSPRAKEK"
     gene            complement(2418..>2620)
                     /locus_tag="VEL68_16875"
     CDS             complement(2418..>2620)
                     /locus_tag="VEL68_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37699.1"
                     /translation="SRSCLLASVASSHYAAMRVRVGPNTPINRTCYSHLRRPPQAGYR
                     QRYPLFQPVIPDLQSCVGLKTR"
BASE COUNT          663 a          695 c          738 g          524 t
ORIGIN      
        1 ccttgataag tatttccagg gagagcagga tgtaaggacg atcgatgtat tagagaagct
       61 caaggagaaa atgggtaaag aaaaagccta aagaggcggg ccgataaggc aagttaaggg
      121 gaggagggaa gatgaaatgc ggaaggacct gattgccaag atcgccctct ccaaggagga
      181 gatcgcccat ctttaccagg gaggtgttat gtctattaag atcaagggga aagacacaaa
      241 cgcctcggtg gaaatatcct gtagggaagt ttctgagcgg aagggcacga gaatccgttt
      301 caagaccatc gacgccgaat agaccgcctt ttgccaaaaa ttccttccat ttatccagaa
      361 tcccgggtgg ccggaagcag accttccatg aagagttggc gaagatttca gttttggccg
      421 gaatgacgcc tttgaacctc aaaagatttt ttcagcaatg tgctattgac agaaagaata
      481 ccttatagta gtaatcttca caattgtctg aagcaggcgg aatttgttga aaaaggaaaa
      541 ggaggggcat tatgagaaaa gcattcctgg ttatggccgt tattctcctt ctgggattca
      601 tgggtggaga ggccgcggcc gcaaggtcgg gaggaacctt caatttctgc gtcccctacg
      661 ggggagacgt tctgaccctc gacccccaca agggcgcacg cgtcaatgac atgattgtcg
      721 ccctgagcct ccaccgcagc ctctacggct gggacccgga aaacaaccgt cccaagctgg
      781 atctgggaga aaaagccgat gtctccccgg acggtctggt gtaccggatt tatctgaaga
      841 agaacgtcaa attccacaac gggcggggga tgaccgcgga tgatgtcatc tggagctacg
      901 agcggatcat gtcccccaag accgcttccc tttccgccgg ttttgtgcgg gtgatcaagg
      961 gggccaagga ttatgaagac ggaaaggccg ataagatctc tggcctgagg aagatcgaca
     1021 atttcaccct ggagattacc atggagaacc ccgtcgatcc cgcttacacc ctctacgaga
     1081 taggaacggg catattgccc cgcgaggagg tggagaagaa aggcttcgac ttcggttccg
     1141 aacccgtggg gtgcggtcct ttcaaattcg tcaaatgggt caagggaagc gaaatcgtgc
     1201 tgaccagaaa cccggattac tacgaaaagg ggaagcccta tctcgacaag ctggtctata
     1261 agatcatgcc cgagggagct tcgcgggatt tggccttccg ggccaaagag ctggatgcca
     1321 ccgtggtggg ttccacccag tacccggctt acaaagcaga cccccagatc tccaaaaaca
     1381 tggtggaggt ggccgagctt ttcacccgga ttataggctt caaccccaaa tttgaaccct
     1441 ttgcgaaaaa acaggtgcgc caggccatca attatgccat cgactcccgc ctgatcatcc
     1501 agaaacttct gaaggaaaaa gccttcccct gcgtgagcta cctgccgtcc acttcgccgg
     1561 ccttcgaccc ccaggccaaa gggtacgaat tcaacctgcc gaaggccaag gcgctcatga
     1621 aagaggcggg gtacgaaaaa ggcttcacct tcgagtgcat cggaatgaac aatgaagcct
     1681 ggggagtagt gatcgtggaa gcgatcatgc cctttctgaa acagatcaac gtgacagtga
     1741 aaccccagca gctcgaggga gccgcccttg ccgatcgggt ccgcaagatg gactaccagg
     1801 gtttcatctg gtctgtggcc agcggcgcgg gcggagaccc actccaggct ctgaaccgat
     1861 ggtcttccaa gaacccaccc acggccggca attttgtagc ctacaacaac cccgagttcg
     1921 acaagttgct ggacgctgcc tcccagatcc gggacgtttc caagcggatt gaccttctcc
     1981 gcaaagccga cgccatcttc cgcgaggatg cgccggtctg gttcttcaac tacaataagg
     2041 cggtggtcgc ccatcagcca tgggttcatg ggatccagac cgtggctatc gagatgatgt
     2101 atcagaacat ggccgatatc tgggtcgaag agtcctctcc ccgagccaag gaaaaataac
     2161 agtgcctgcc gcacctgccg gcaaataaga aagaaaccag cttgctcccc tcaaccccgc
     2221 tcgccgggcg ggattgaggg gggccgggct cacacctgtt cccggggcgg atggggcttt
     2281 ctcctctgag ggcccgcagc cctttatcgg tatgtttggc cgacaaggaa aggtgtaaaa
     2341 gggcggggct agactcatta ccttggcccc acccagcctt cgctctttga aaattgaata
     2401 tccctttttt gctggcttca ccgagtcttc agcccaacac agctctgaag atcaggaatt
     2461 actggttgaa agagtgggta acgctgccga taaccggcct gcggaggccg gcgaagatgg
     2521 ctgtagcagg tccggttgat tggcgtgtta ggccccacgc gaactcgcat tgccgcgtag
     2581 tgagaagaag ccaccgacgc caataagcac gaccgcgaca
//
LOCUS       DASPRR010000461        22684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1883004, whole genome shotgun sequence.
ACCESSION   DASPRR010000461 DASPRR010000000
VERSION     DASPRR010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 22684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22684)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22684
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883004"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(906..1763)
                     /locus_tag="VEL68_16880"
     CDS             complement(906..1763)
                     /locus_tag="VEL68_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXZ37700.1"
                     /translation="MRDKRPMGMLKRLRDGAIPISKAIINPIFVTIFLFCLAAAFPAT
                     AEESRLQSILQKKEIRVGTSGDYQPFSYLNPQNNQYEGMDIEMAHKLGEALKAKVVFV
                     RFKWPELAADLLADKFDIAMGGIGRNVERGKTFAYTNSFMTFGTCPLVRKGDENKFPD
                     FASINRAGVKVLLNQGGINDRHFTPLLTQATILRHNKNEEIAGKVKEGEADVWITDNV
                     EALFWAKQTPGLVAVNPAKTFTVGTKGYMIRQGDQIFLNWLNLWLEQMFLQGEVQELE
                     QQWLGMVMR"
     gene            complement(1844..2401)
                     /locus_tag="VEL68_16885"
     CDS             complement(1844..2401)
                     /locus_tag="VEL68_16885"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HXZ37701.1"
                     /translation="MFEELLVRIASLLSRGNLPYMIIGGQAVLLYGEPRMTKGIDITL
                     GIDVDRLDELLAIVKELSLTPLPEDILSFVQKAMVLPVLDKSTGIRVDFIFSFTTYES
                     QAIGRAKKIILSGQEVCFAAPEDLILHKMFAGRPRDLEDVSTLILKNPGIDVAYIRRW
                     LKEFDAAVQGKGFLEAFERILKGRG"
     gene            2909..3307
                     /locus_tag="VEL68_16890"
     CDS             2909..3307
                     /locus_tag="VEL68_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37702.1"
                     /translation="MIAIIALKEDFSMQCPECGRRIPDNSDSCLYWRVWIKGEASSDA
                     RTEPSTISVSSGKMGENITGIQVTKEEINFKKLEGLPLSIRARVEEILKKGEGQSEEI
                     ETSFRNIPESKERAGRKRKRMGSLDPSNSF"
     gene            complement(3430..4296)
                     /locus_tag="VEL68_16895"
     CDS             complement(3430..4296)
                     /locus_tag="VEL68_16895"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXZ37703.1"
                     /translation="MIEKRPFGRTGHMSTATIFGGAALIRATPKDADRVLEILLKYGV
                     NHIDTAPRYGDSEVLIGPWMARHRKDFFLATKTGKRTHQEAREEIHRSLDRLKTDRID
                     LIQLHGLQHPDEWDTALGPEGALEAAVEAREKGLVRFIGITGHGWMIAALHKRALKRF
                     HFDSVLMPYNYFMHSNERYRNEFEEVVGICRERNVAVQTIKSLARGPWAITPQNRNTW
                     YQPLEDQDDIDRAVHWVLGHPNVFLNTVGDVTLLPKVLDAASRFQRRPSPEEMDEMVK
                     RNGMSSLFGIGT"
     gene            complement(4323..5003)
                     /locus_tag="VEL68_16900"
     CDS             complement(4323..5003)
                     /locus_tag="VEL68_16900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ37704.1"
                     /translation="MKRALCIIALGVWGVLSAFSIPCYPQAGEKEKIIDPKVEKFLQK
                     MRNQWHDWNVPEADGKILYEIIVKNNYKKAVEIGTSTGHSGIWIAWALSKTGGKLITI
                     EIDEGRYRQALANFKEAGLSEYIDARLADAHQLAKELPGPFDFVFIDADKDGYSNYAK
                     ALIPKLEPGGCLTAHNVWETQTGPRGGYFSGTEEYYRFMKSIPDFETSIHPNSSAGVA
                     VSYKKKMK"
     gene            complement(5184..6071)
                     /locus_tag="VEL68_16905"
     CDS             complement(5184..6071)
                     /locus_tag="VEL68_16905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ37705.1"
                     /translation="MYDFAKGSVKFIRGGRYPHCNSVLVDDRVRAVIDAASDESRLLF
                     FEKQNRIDYLITSHAHEDHLVYNYLFSESTFWAHPLDAPHFEDVKSLADCYGDMPSEA
                     FQKWCDFFVTECHYVPRKVDRFLEDGRILDFGETRMEVIHTPGHTEGHCVFYFPQEKI
                     LFTADLDLTRVGPYYADRTSDIEKTIHSLERIKTYPVEIYLTAHGKGIYEGDPAHINR
                     YLSMIFFREERLIDFLRQGAKSLEEITAEGIIYGKNPAFLGAWDLTISERMMIAKHLA
                     RLIRLGGVRKEDDLYVLSE"
     gene            complement(6252..7439)
                     /locus_tag="VEL68_16910"
     CDS             complement(6252..7439)
                     /locus_tag="VEL68_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXZ37706.1"
                     /translation="MPGPLQGVRVLDMTWALAGPFCTMLLADLGAEVIKVENPDEGDP
                     SRKNFPFIQGVSSYFLSVNRGKKSITVNLRHPKGKEVILGLVRKSDVLVENFRPGVMD
                     RLGLGYKALREVNPRIIYASCSGFGQTSPYANRPAYDVIVQGMGGTLSITGEAGGGPI
                     RVGFSIGDIGGGIFTSLGILSALHERERSGEGQMVDVSMLDCQVAFLENAFARYFATG
                     EVPERIGTRHPVLTPFQAYPTRDGYLVVAAARDPFWQKFCKVIQREDLVSDARFKENP
                     IRTKNHKELEKEVTEALKSKTTAEWVDLMIQEDIPCGPVNSVPQLAKDPHTLAREMIA
                     EVKHSKVGNLKVVNNPIKLSRTAVKLEKANPELGENTEEILTGLLGYGLEEIAGLKSE
                     KAI"
     gene            complement(7551..8300)
                     /gene="fabG"
                     /locus_tag="VEL68_16915"
     CDS             complement(7551..8300)
                     /gene="fabG"
                     /locus_tag="VEL68_16915"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HXZ37707.1"
                     /translation="MRLKDKVAIVTGAGRGIGEGIALRFAEEGAKVIVNDVNEGDAKR
                     TVETIKGKGGKAVAVVGSVTSREVAQRMVDTAVKEFGTVDILVNNAGITRDAMLHKMT
                     DEQWDQVIEVNLKGVFLGIQCAGRVMREKGYGKIINISSTSWRGNPGQLNYSATKAGV
                     IGMTKTAAKELAPKGINVNVIAPGMIWTDMIKAMPPAIVERMEKGLAFIVPLNRKGNP
                     QDIANLALFLASDESSFITGQLIHCDGGMIM"
     gene            complement(8500..9972)
                     /locus_tag="VEL68_16920"
     CDS             complement(8500..9972)
                     /locus_tag="VEL68_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ37708.1"
                     /translation="MTKSIEPNFDLLFNPKSIAMIGASSNLGKWGAIVFLNMIMGGYQ
                     GKLFPVNPKGEMVFNRQVYPRVTEIPEPVDLAVIAIPAQFIKEAVQDCIQKGIRMAIV
                     ITSDFSETGEEGARLEREMVEIARSGGLRLVGPNTMGIFSASTSLTALMPPVRPRRGS
                     VSLVSQSGNIGTQMLGRGEKFAVGFNKYVSSGNEGDLRSEDYLDCLGRDPDTKVILLY
                     IEGLDDGREFLSKARNITPHKPIIAFKGGKTEAGTRAAKSHSGAMAGVKEIYEAAFRQ
                     AGILWASTTEEMLELAAAFSSLPLPRGNRVGILTRGGGWGVITADACNELGLEVPPLD
                     EAIIHRLNNLLPAFWSRGNPVDMVATMGMDPYIQCLEALISWEKVDAVISLSGDAGPL
                     AHLLPDIKKKAEGIIPTDKTEKMMQYVSESRSRIYDRVCELVERHRKPVFAVGANFPR
                     GKGTGKSDFTLAQFRSPERAAKAAGMMYRYYQYRKAIGME"
     gene            complement(10130..10756)
                     /locus_tag="VEL68_16925"
     CDS             complement(10130..10756)
                     /locus_tag="VEL68_16925"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ37709.1"
                     /translation="MIRRVDHVSIAVRDQTKAKAFFIDVLGGKNLFEAPVPWQKFRWT
                     TIELGTSCFIELIDPLEKDSFVQRFLDNRGEGPHHITIQVNDIQEVHRKLEENGIPTF
                     GLAEPFPNWKEMYIHPKHAFGTLIQFAEFDPLDWINPGYVPPSYAEFASPDRVGPKDG
                     GVEVDRVQGEKGPEVEIRQSGQKIRVPLSQLGYLIQTLKKQQPASESA"
     gene            10997..11620
                     /locus_tag="VEL68_16930"
     CDS             10997..11620
                     /locus_tag="VEL68_16930"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXZ37710.1"
                     /translation="MGNRMTAEDRRGQILRGAMELFAEKGFRGATTREMAQHLGISEA
                     LMFKYFPSKEALYRAIIQKRTDGAEDVFFPQEAVQAKDDRQVFQSIASNLIARNTEDP
                     TFMRLILYSALEGHELSRIFFETHVMGQTRLLSDYIRQRIKEKAFKPVHPLLAARAFM
                     GMIIHYVEFQEIYGMKGFLKFSQKKAVHTFVDMFLNGLTGNKKKNGA"
     gene            11661..12908
                     /locus_tag="VEL68_16935"
     CDS             11661..12908
                     /locus_tag="VEL68_16935"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXZ37711.1"
                     /translation="MILVGVVCLGIALYFGVQWLLFRWHYVSTDDAQVKGNLVNLSAK
                     VSGRIVQMRVEEGDPVQVGQVLVEIDPKDYQASQAQAQANLEMARHDLAKAITQLSLT
                     KERVIQGIGTAETSLREAREGLKFAEEDAALQTDRVNKDIDRARASLQAARARVVEAR
                     ATMENAKKEFERNHELFRQNYVAENTRDTAETAWQVGQSKYQAAVENEREALSQLELA
                     EANQRSIDLKKQSIRIAEQSLERAKINLALAQEEKQQITLQEKSIEFLKAKVQETEAA
                     LQLADLRLQETKIQSPISGTVSKRLADQGQVVQVGQPLLVVNNPGEKWVVANVEETKV
                     RRVHPGAKVTVEVDAFPGNAFEGQVEFIGSAASSEFALLPSENPSGNFIKVTHRIPVR
                     VSVKDPKNKLKPGMMVVVAIEAK"
     gene            13259..14824
                     /locus_tag="VEL68_16940"
     CDS             13259..14824
                     /locus_tag="VEL68_16940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXZ37712.1"
                     /translation="MNNFSHQGKDHKWGVLGVVVLGSFMAILDNNIVNVALPKMMANF
                     GATVDQIEWVVTGYMIAFAISMPTTNWLKEMIGLKKLFIFSLVFFCLGSALCGMAWDK
                     DSLIGFRVLQALGGGALMPTGLTLVSEAFPPQERGMAMGTWSIGAMVAPAAGPFLGGY
                     LVDEVSWRSIFYINLPVGAVAILMALWILPSVRPANPSRRLDLTGFISLSVFLAFFLI
                     ALAQGQREGWDSDYIIACLSLSVVGLVAFVASGFLVKDPIIDLHLFANHNFVMANIIN
                     LARAVAIFGAMFLLPLFLQNVLDYKALHTGIILAPTAISVAIVSPFSGLISDRIGPRI
                     PLFCGTVLVAFSLYMYKDLSLDSDYWFLFWPQVIRGAGMGLLNAPLMSAALNAVRREQ
                     TSIASSLLTVTIQVGGAIGVALLATTLERRQFFHYTHFLEHINNASTPMVIQAQSAME
                     ALLARAGYAPADFLGKGKTLLALWMQKQAAVGAFEDAFVLAGLLITVGFIPALLIRNR
                     HGSQGSKPLSIGE"
     gene            14938..16305
                     /locus_tag="VEL68_16945"
     CDS             14938..16305
                     /locus_tag="VEL68_16945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXZ37713.1"
                     /translation="MATKGMTVWALNLIFLLFWGGWALAQEGLIPPPGKPLTLDQCVA
                     IAVKIHPALAANQATVEAQKALVEQALAAYYPQLNYNTSYSAFTTNFSTSPISNPGGG
                     AIGVPTTGPNRYRWAYTDVVSTGPTATQTIWDFGRTPNNVRINRENVKASLEDLITTK
                     QTVILNVQTAYYGLLQTQYLIDVAQDTVNQTQLHLDQAQGFYQAGTQPKIYVTNAEVA
                     LANAQLALIQVKNNSSVARVTLNNAMGLRQDLNFEIEKKIDIAPKVTPLEEILQTAYE
                     KRPEILKLKAQQSSQEAAIKLAKDGYYPILSGNASLLYRSTALDLSQPFYWDSFFSAT
                     LSIPLFSGFLTPNQVSQQRAVLMNLKSQEESTRQNIRLEAEQAYLSLKVAIDQMAVTQ
                     KTVEQAKENYDLASGRYQVGVGSPLEITDAEVSLANARANYIQAVYTFKTAEARIEKA
                     MGINR"
     gene            16390..17637
                     /locus_tag="VEL68_16950"
     CDS             16390..17637
                     /locus_tag="VEL68_16950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ37714.1"
                     /translation="MKKTIIVLAILAVAGLGAFRAAQVISARKTEDPKRQGMGQVPLV
                     EVAPVTQGLIEEKMLRTGDIAPFAQVVIYSKVQGWVEKINFREGDRVRAGQVLATLDA
                     REAEATVAQSKASLEAAVARLKQVKATSEETIQSQIQQAKANLDFAAEDLKRFQELHQ
                     KDFIARQQLDDARTKYSVAKANYDLALNNVRQKTWENDIALAEAQVQQAKATLDLNQA
                     QLANLIILAPMNGKITKRYVDPGIMVKDTTPILAMMDLDEVKMLVNVIEKELVRLQKG
                     QEVKVTVTAFPDRTFQGRIAIITPALDLQSRTAEIQITIPNPGYILNPGMFGRVEALL
                     RADPKATLIPIQALITEEDKDFVFALKDGKVYRRPVQKGISKDTVVEIIQGLNPGDQV
                     VTAGQEFLRDGIPVRLAAKSEKK"
     gene            17655..20795
                     /locus_tag="VEL68_16955"
     CDS             17655..20795
                     /locus_tag="VEL68_16955"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXZ37715.1"
                     /translation="MNLARFALRNPITILMAVLMAIILGVISFSRMPVDMFPEITFPS
                     ITVATFYKGANPQDIERMVTYPVEKAVSTVNGVKYVQSISRQGASLVTIYFNWGTNLD
                     SAQSDVLQVVNLARADLPKEVENPIIRKFDVSQISVVFITLMGGGLNEGQLYDLAYNV
                     VEPEIEHVTNVASARVVGGMVREIYVHFYRERLQAFNLSPDDVIQAVQQSNLILPAGD
                     IKAGPYDYRLSTETQFSLVKPMENIIVSNFQKRPVYVRDVGEVKDYYQDQTNLIRVNG
                     QPAVALSVQKTSGTNTIQVVDDVRKALPRIKKLLPPGVVMLELFDQSLYIRNSIKSLQ
                     HEALMGAVLAILVILVFLRNLRSTMIVSLSIPISIVCAFILLYFNNLTLNTFTLGGLA
                     LGVGRLVDDAIVVLENIYRHRSAGESPQEASIKGSGEVGLAVLASTITTIVVFLPVIF
                     ITGIARLLFTPLALTVAFSLIASYFVSLTVIPVLSRKYLHPESEEVLPPTPTALERTK
                     WRFKVLFERIDSGYQSLLAWVLGHRKTVVIAVVVAFLGSLPLYLFIGTEFFPSMDESQ
                     FRFIVQLPVGTRLEEAGRMAARMEEVVRETIGKEITAVQVNFGIPTGVSAIWSQNTGP
                     HMGWIRVRLIDPGERGLSSDALMEKLRPRLLQEFPGVRIFFVAGGIVRMLISFGYDNP
                     IDVEILGYDFKAGQKLADQVGAIIRQTPGAKDVRISREPDYPQQNIVIDRERASLLGL
                     SVAQVARAIQTFINGYQASIFSDPGTGNQYYITVRAHGADRTSVSDLDQIFLFNRDGR
                     PISLDNIANISRGVGPIQIERKYQQRVIHVTANTFGRDLGSVAADIQASIDKLQLPPN
                     FKINMTGAVESQRESFIALLGALILAVVLVYMVLASQFKSLIDPFIIMFSVPLGLIGV
                     LWALFLTQTNLSVTSFMGVIMMAGIVVSNGILLVDYTNRLRGRGMSLEEAVTLGGRTR
                     LRPILMTTLCTILGLIPMAIGLGEGSESNAPMAIAVIGGLSVSTLLTLVFIPTLYTIF
                     ESRFKREIHTEEKEQP"
     gene            20792..22414
                     /locus_tag="VEL68_16960"
     CDS             20792..22414
                     /locus_tag="VEL68_16960"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ37716.1"
                     /translation="MKKKITFWIILILLLAGVALGYTYYYRGKDSAVKYRTARVERGN
                     LSAFVTANGTINPVITVTVGSQVSGTIQKLYADYNSQVKLGELVALIDPAIFQAQASQ
                     AKAKLENARATFLNAQADISTAKANVESSRANVIKAKVSVDDTKRSLGRSLELFSRNL
                     ISASDRDASQTAYDSAVALSEAAQAQLSASEAQLESARAKLEAARAQVKQSEAELELA
                     QVNLDHCKITAPVTGIVISRNVDVGQTVAASLQAPTLFTIAQDLTEMQVDTNVSEADI
                     GRVAIGQEATFTVDAFPNLTFLGKVTDIRNAPVTVQNVVTYDVVILVKNPELKLRPGM
                     TANASILVAQSENALKIPNSALRFRPDFARKEAEASPKGQVSPSPPPPSATSPEQILE
                     RLKAELKLTPQQEAGILSILKDAQGEILAARKAGGSEEAKNKAKELRAANRIKIRSLL
                     TEEQKKKYDQLDRRPDSSQGATPMYRVWLAVPEGRPVPVDITTGISDGSYTEVTSGAL
                     KEGQEIILEAMAGNGKSGGSSQAPQPSMKGFR"
     gene            22529..>22684
                     /locus_tag="VEL68_16965"
     CDS             22529..>22684
                     /locus_tag="VEL68_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017287753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ37717.1"
                     /translation="MSELIRVEHLTKIYYLGEVQVPALKGMDLTIEPGECVAIMGASG
                     SGKSTFMN"
BASE COUNT         5165 a         6219 c         5984 g         5316 t
ORIGIN      
        1 gggttaatct ttcggcatct ctatcgtctt ccgcgtcggg ccgccttaaa atctcaaccc
       61 ctcttttcac tacccgctca tgaacaatta acggccttcg acgattatct tgggtgaaac
      121 ggagctcata ctcccaatcc gccgtatttt tcaaatctcc gaggtttatc cgaacgataa
      181 tatccgaata tcgccgggcg gccagagcgc gaaggccgga tacccccccc cgctttcgga
      241 ccgcttccgc aaaacctccg ccgatggaaa cgatatcatg gagaaaccga aaaacgtcga
      301 gaaaatttga ttttcctgat gcgttggggc ccacgaggaa tacgcgacgt tgaagatcag
      361 catctgcccg aaggaaattt cgccaattct ccaaataaat atgggagaac tgcaatattt
      421 tttttctttc atgatttctt cttgccatgt tcatacgtta tgctcctgag aagagcaata
      481 agaagaggat tctaaactcc taccgaaatc gcttaccacc ggtttgaacg ctataaacaa
      541 aaccctaaat tcaatccctt ttcactcgcg cgcaccagat gatattgccc cgtttatgcc
      601 cctcattccg ccgtctcccc cagagctaca tagaatcagg gatgagaatc tgcgtaactg
      661 atgggattct atacaagaat tatcaatata tcaaggattg tttctggtga ctccctgaaa
      721 atcttctttc cagcgcctgc gcgaacctag aattcacgca acgcggttaa aaacttgatt
      781 cctgttctct gcccaagaca tggaggaacg agtttcacga gaacaacaaa aaagagatct
      841 tattgacttt ttagaggaat atctcctttg tcttattgtg gggcagtggc tgctgcagtc
      901 ccaatctacc tcatcaccat ccccaaccac tgctgttcaa gctcctggac ctcgccttgt
      961 aggaacattt gctccagcca gaggttgagc caattcagga aaatttggtc gccctggcgg
     1021 atcatgtagc ctttggtacc tacggtgaag gttttagctg ggttgaccgc gaccaatccg
     1081 ggggtctgct tggcccagaa cagggcttct acgttgtccg tgatccagac atcggcctcg
     1141 ccctctttca cctttccggc gatctcttca tttttgttgt ggcggagaat ggtagcctgg
     1201 gtgaggaggg gggtgaagtg gcggtcgttg attcccccct ggttgaggag gactttcact
     1261 ccggctcggt taatggaggc gaaatctggg aacttgttct cgtccccctt gcgaacgaga
     1321 gggcaggtcc cgaaagtcat gaaggaattg gtgtaggcga aggtcttgcc ccgctctacg
     1381 tttcggccga tcccgcccat ggcgatgtcg aatttgtcag ccaaaaggtc cgccgccagt
     1441 tccggccatt taaagcggac gaacacaacc ttggccttca gcgcttcgcc caatttatgg
     1501 gccatttcga tgtccatgcc ctcatactgg ttattttggg ggttcaaata agagaaaggc
     1561 tgatagtcgc cggaggtgcc gacccggatt tccttctttt gaaggatact ctgtaacctt
     1621 gattcttcgg cggtggcggg aaaggccgcc gccaggcaga agaggaagat cgttacgaag
     1681 attggattga tgatagcttt ggaaattgga atagcgccat cccggagcct ctttaacata
     1741 cccataggac gcttgtccct cattctttct ctgcagtcga acgatgcatt ctttcgagcg
     1801 aagaaataga ccctgccctt gcggccaagc ctcccttccg cttctacccc cttcccttaa
     1861 gaatcctttc gaatgcctcg agaaacccct tcccttgaac cgccgcatcg aattccttca
     1921 accagcgcct gatgtacgct acatcgattc ccggattctt caggatcagg gtgcttacat
     1981 cctcaaggtc tcggggtcgt cccgcaaaca tcttatgaag gataaggtcc tctggggcgg
     2041 cgaaacagac ttcctgtccg gagaggataa ttttctttgc ccttccgatg gcctgcgatt
     2101 cataggtagt gaatgagaag atgaaatcca cgcggattcc tgtgcttttg tccaggacgg
     2161 gtaacaccat tgctttttga acgaaggaga ggatgtcttc cgggaggggc gttaaagaaa
     2221 gctctttgac gatcgccagc agttcatcca ggcggtcgac atcgatcccc agggttatgt
     2281 cgataccttt ggtcatccgg ggttcgccat agaggagtac agcctgtcct ccgatgatca
     2341 tgtaaggcag gtttcctcgg gaaaggaggg aggctattcg gacaaggagt tcttcaaaca
     2401 atttagcacc cgtgcgagct ccaaatcagc ttcaattccg tccaggggat cggaaagagg
     2461 gaggaccccg agggccaccg cttctttcca catcgcttca tataacttca gggcttcctg
     2521 gaaagagcgc tgaggttccc ttctaataag gtcctcttcg aaacgcctaa gagtatttgc
     2581 gtcccggatc attgccttct ccacctcttg tgccaacaca atttcacccc ggcctggtac
     2641 ttttcccagg ccattcattt ctcatttaat caaaaaagga gggcgggtgc aatatctata
     2701 tccttaagaa tggacaactt ccctggtttt ataccaattt ttatcccctt ttttcctttc
     2761 ctttcgtcct cgggggcaaa ccaagaaaat ggaagagagc gacgacttta aggaacttat
     2821 tctaatctgc cgatatatct aatgttgggc aggtccgacc acgcgaaatt ttaggaatta
     2881 tcataactga tggaaaccgc tttaaatttt gatcgccata atagcattga aggaggactt
     2941 ctccatgcaa tgtccagaat gtggccgcag aataccggat aacagtgata gttgcctgta
     3001 ctggcgggtg tggatcaagg gggaagcctc ctccgatgcg aggacggaac cttcgaccat
     3061 atcggtctcc tccggaaaaa tgggagagaa tattactgga attcaggtaa ccaaggagga
     3121 gataaatttc aaaaaactgg agggacttcc tctatccatc agagccaggg tagaagaaat
     3181 actcaaaaag ggagaagggc agagtgagga aatagagact tccttccgca atattcctga
     3241 atcgaaggaa agggcaggaa gaaagagaaa aaggatgggt tccctggacc cctcaaattc
     3301 cttctgaaaa aagaataatc cttagagaaa aaaagactgc gggtacccaa cacgccaggc
     3361 ggcccgatga taaatccccg gtccgctcat ctacgccgga aatggggtcg ccgcttgaaa
     3421 acctggtttt taagtgccga ttccaaaaag cgatgacatt ccattccttt tgaccatttc
     3481 atccatttct tcgggtgacg gacgcctttg aaagcggctg gcggcgtcca gaactttggg
     3541 cagaagggtc acatctccca cggtgtttag gaatacattc ggatgaccta acacccagtg
     3601 aaccgccctg tcgatgtcgt cctgatcctc cagcggctgg taccaggtat tccggttctg
     3661 cggggtgatg gcccagggac cgcgggcgag ggatttgatg gtttggacgg ccacgtttcg
     3721 ttcccggcag atgcccacca cttcctcgaa ctcattccgg taccgttcat tcgagtgcat
     3781 gaaatagtta tagggcatca ggaccgaatc aaaatgaaag cgcttcaagg ctcgtttgtg
     3841 cagggccgcg atcatccatc cgtgtccggt gattccaatg aagcgcacca agcccttttc
     3901 ccgggcttcg accgccgctt cgagagctcc ttcgggtccc aaggccgtgt cccactcatc
     3961 ggggtgctgc agaccatgga gctggatcaa gtcgatcctg tctgtcttta ggcggtcgag
     4021 ggagcgatgg atctcttcgc gggcttcctg gtgggtcctt ttccccgtct tggttgccag
     4081 aaagaagtct ttgcgatgcc tggccatcca ggggccgatg aggacttccg aatcaccgta
     4141 acgcggcgcg gtatcgatgt ggttcacccc gtacttcaga aggatttcaa gaacccgatc
     4201 cgcgtctttg ggggtggccc ttatcagggc ggcccccccg aagatcgttg ccgtgctcat
     4261 atggccggtg cggccgaaag gtcttttttc gatcaatttg ctgtctcctg gtcgattgaa
     4321 ttctatttca ttttcttctt gtaactgacc gcaacgccgg cactgctgtt cggatggatg
     4381 ctggtttcaa aatcggggat gcttttcatg aaccggtaat attcctcggt accgctgaaa
     4441 tatcccccgc gggggcctgt ctgagtctcc cagacgttgt gcgccgtgag gcagcccccg
     4501 ggttcaagtt tggggatgag tgctttggcg taattgctgt acccgtcttt atccgcatcg
     4561 atgaagacaa aatcaaaggg cccgggcagc tcctttgcca actggtgggc atcggccagc
     4621 cgggcatcga tgtactcgct aaggccagcc tccttgaaat tagccaaggc ctgcctgtac
     4681 cgcccctcgt cgatttcgat ggtaattaat ttccccccgg tcttgctgag ggcccaggct
     4741 atccagattc ccgaatgccc cgtcgaggtg ccgatctcca ccgccttttt ataattattc
     4801 tttacgatga tctcatagag aatcttccca tccgcttccg gaacgttcca atcgtgccat
     4861 tgattcctca ttttctgaag gaatttttca acctttgggt cgatgatctt ttccttttcc
     4921 ccagcctgcg ggtaacaggg gatggagaaa gctgacagaa cgccccatac gcccaaagca
     4981 atgatgcaaa gcgccctttt catccgactc ccctccctta ttcagtataa caaaccagcg
     5041 gaggaaactc caaagtcttc tattaattgg gttgggaatg ggcgggagag cgaaaggggt
     5101 gggtgatttt ccttccaaat ggtttttgtt cggcccggct aggttaacat attgattttg
     5161 tgagcaaaaa agattgccct tgattattca ctcaggacat aaaggtcgtc ctcttttcgc
     5221 acgccgccca gtcggatcag gcgggcgagg tgtttggcga tcatcattct ttctgagatg
     5281 gtcaggtccc aagctccaag gaaggcaggg ttctttccat agatgattcc ctcggcggta
     5341 atctcttcca aagacttggc cccctggcgc agaaaatcga tcaacctttc ttcccggaag
     5401 aaaatcatgc tcaggtaccg gttaatatgg gccgggtctc cctcataaat accttttccg
     5461 tgagcggtaa ggtatatttc cacgggatag gtttttatgc gttccaggga gtggattgtc
     5521 ttttcaatgt ccgaagtccg gtcggcataa taggggccca cccgggtcaa gtccaaatcc
     5581 gcggtgaata gaattttctc ctggggaaaa taaaaaacgc agtgcccttc cgtatgtcca
     5641 ggggtgtgga taacttccat ccttgtttca ccgaagtcca agatcctccc atcctcaaga
     5701 aaacgatcca cttttcgggg aacgtagtgg cactcggtca cgaagaagtc gcaccatttc
     5761 tggaaggctt cggaaggcat atccccgtag cagtcggcca aggacttcac atcctcgaag
     5821 tggggggcat cgagagggtg ggcccaaaag gttgactccg agaagaggta attgtagacc
     5881 agatggtctt cgtgggcatg agaagtaatc aggtaatcga tccggttctg cttttcaaag
     5941 aaaagtagcc tgctttcatc gctggccgcg tcgatgaccg cgcggacccg gtcgtcgacc
     6001 aacaccgagt tacagtgggg ataacggccc ccgcggatga attttactga gcccttggca
     6061 aaatcgtaca atttgtgagg ctctcccgat attttcttgg agagggatac cccttaaatt
     6121 caaaatccca atatcgaaat ccgaaacaaa tccgagtgac caaaatccaa aacgagtggc
     6181 tttttttgaa catttcgttt tcggattttg aaattgtatc gagattcgtg tttagagatt
     6241 caaaattaac cttaaatcgc cttttccgac ttcaggcctg cgatttcctc aaggccgtag
     6301 ccgagcaacc cggttaaaat ctcctcagtg ttttctccta gttccgggtt ggccttttcc
     6361 agcttcaccg cagtccggga gagcttgatc ggattattca ccactttgag gttgcctacc
     6421 ttagagtgtt ttacctcggc gatcatttcc cgggccagag tgtgtgggtc tttggccagc
     6481 tggggaacgg aattcaccgg cccgcagggg atatcttcct ggatcatcag gtccacccat
     6541 tccgccgtgg ttttgctttt caaggcttcg gttacttcct tttccagttc cttgtggttt
     6601 ttcgtgcgga tcgggttttc tttgaaacgg gcatctgata ccaggtcttc tctctgaatc
     6661 accttgcaga atttctgcca gaatggatcg cgggctgcgg cgacaaccag atatccatcc
     6721 cgggtgggat aagcttgaaa gggagtcaac acggggtggc gcgttccgat tcgctctggg
     6781 acctcccccg tggcaaaata ccgggcaaag gcgttttcga ggaaggccac ctggcagtcc
     6841 agcatagaga catcgaccat ttgtccttcc ccactcctct cccgttcgtg gagggcggag
     6901 agaattccca gggaggtgaa aattccgcca ccaatgtccc cgatggaaaa tcccacccgg
     6961 atgggtcccc cgccggcctc cccggtgatc gacagggtcc ctcccatccc ctggacaatc
     7021 acgtcataag ccgggcggtt cgcatagggg ctcgtctggc cgaaccctga gcaggaggca
     7081 tagattatcc ggggattgac ttcccgtagc gccttgtatc ccaggcccag gcggtccatc
     7141 accccgggtc gaaaattttc caccaggaca tcggattttc ggaccagccc cagaatgacc
     7201 tccttaccct tggggtgccg cagattcacg gtgatgctct ttttgccccg gttgacgctc
     7261 agaaaataag aactgactcc ttgaatgaag ggaaaattct ttctcgaggg gtcaccctca
     7321 tctggatttt ccaccttgat gacctcggct cccagatcgg ccaggagcat ggtgcagaaa
     7381 gggcccgcca gagcccaggt catatccaga actcgaacac cctgcaatgg accaggcata
     7441 aaaacctcac caaaaaggaa tccagaagcc agaattcagg agtcagaaaa aaggccgaaa
     7501 ttctattctg gattctggct cctggctcct gaatcctgaa tcctgtttta ttacatgatc
     7561 attccgccgt cgcagtggat gagctggccg gtgatgaagg agctctcatc ggaggccagg
     7621 aaaagcgcca ggttggcgat atcctgggga ttccctttcc gatttagagg aacgataaaa
     7681 gccaaaccct tctccatacg ctccacgatt gccggaggca tggccttaat catatcggtc
     7741 cagatcattc ccggagcgat gacattgaca ttgatcccct tgggagccaa ttccttggcg
     7801 gcggtcttgg tcattccgat cactccggcc tttgtcgccg aataattcaa ctggccagga
     7861 ttccccctcc agctggtgga ggatatgttg atgatcttcc catatccttt ctcccgcatc
     7921 acccggccgg cgcactggat accgaggaag actcctttca gatttacctc gatgacctgg
     7981 tcccactgtt catcggtcat cttatggagc atggcatccc gggtaattcc ggcgttgttt
     8041 acgagaatat ccacggtccc gaattccttt accgccgtat ccaccatcct ctgggccact
     8101 tcccgggagg taacgcttcc gacgactgcc acagccttcc ctcctttgcc cttgatcgtc
     8161 tctaccgtgc gtttggcatc tccttcgttc acgtcattca cgatcacctt ggccccttcc
     8221 tcggcaaagc gcagggcgat cccttctccg atccctcttc cggccccggt cacgatcgct
     8281 accttgtcct tcagtctcat tttccttacc tccctttttt gttattccaa tctattttaa
     8341 taatccgacg ctgtttatcc gcagattgcg cggattacgc agaaaggaca gaggcataaa
     8401 tccaaggatt aggaatggca ttacgatcat cgaagcttaa accaacttgc cccaaccata
     8461 tctctgtcct tagctcttca tccgtgtcca attaagtttt tattccattc cgatcgcttt
     8521 ccgatactgg taataccgat acatcatccc ggctgctttg gccgcccgct ccggagatcg
     8581 gaattgggcc agagtgaaat ctgatttccc cgtcccttta cccctgggga aattcgcacc
     8641 cacggcaaag accggcttcc ggtgtctttc taccaactcg cagacccggt catagatccg
     8701 cgaccgagac tcggaaacat attgcatcat cttttcggtc ttgtccgtcg ggataatgcc
     8761 ctccgccttt tttttgatat ccggcaggag gtgggccaac ggcccggcat ccccgcttaa
     8821 ggaaatcacc gcatccacct tttcccagga aatcaaagct tccaggcact gaatgtaggg
     8881 gtccattccc atagtagcca ccatgtccac cggattcccc cggctccaga aagcggggag
     8941 aaggttgttc agcctgtgga tgatcgcctc atccagggga gggacctcaa gtccaagctc
     9001 attgcaggcg tccgcggtta tgactcccca tccgccccct cgggtgagga tgcccacccg
     9061 gtttcctcgc ggaagcggga gggaagaaaa ggctgcagcc agttccagca tttcttcagt
     9121 agtagaggcc cataatatgc ccgcttgccg aaaggccgct tcgtaaattt cttttacccc
     9181 ggccatggcc ccgctgtgag acttggctgc ccgagtaccg gcttcagttt ttcctccctt
     9241 gaaagcgatg atcggtttgt gaggcgtaat attcctggct ttgcttaaaa actcccttcc
     9301 gtcatcgagc ccttcgatat aaagcaggat gaccttggtg tccggatccc tgcccaggca
     9361 atccaaataa tcttcggacc tcaggtctcc ttcgttcccg ctgctcacat atttattgaa
     9421 gcccacggcg aacttctctc cccgtcccag catctgggtg ccgatattcc cgctctggga
     9481 tacgagagag accgatcccc ttctgggcct aaccgggggc ataagagcgg tcaggctggt
     9541 ggaagcgctg aaaataccca tggtgttcgg ccccaccagc cgcaaccccc cagagcgggc
     9601 gatttcgacc atctctcttt ccagccgggc gccctcttct cccgtttcgc taaaatccga
     9661 ggtgatcacg atggccatcc ggattccctt ctggatgcaa tcctggacag cctctttgat
     9721 gaattgggcc ggaatggcga tgaccgctaa atccacgggt tccggaatct cggtcaccct
     9781 gggatagacc tggcgattaa aaaccatctc tcccttgggg ttcacgggaa aaagctttcc
     9841 ctgataccct cccataatca tattgagaaa aacaatggcc ccccactttc cgaggttact
     9901 ggaggctccg atcatagcga ttgatttcgg gttgaagagc aggtcaaaat ttggttcgat
     9961 cgacttcgtc atttgaatcc cttttgaatt ttaggcgctg tcgtctcccg aaaaaacagt
    10021 aggggcgggt ttgaaacccg cccttacgga tttcggcctt tctgttcccg accctatatt
    10081 ggtcaaagag ataaagaatt gtcaagtagg ggcaattcat gaattccccc tatgccgact
    10141 ctgaagccgg ttgttgcttc tttagcgtct ggataagata tcccaactgg ctcagcggaa
    10201 cccggatctt ttgcccgctc tgccggattt ccacttccgg ccctttttcc ccctgaactc
    10261 tatcgacttc cactcctcca tccttcggcc ccacccggtc gggagaggcg aattccgcat
    10321 aggaaggcgg gacatacccc ggattgatcc agtccagggg atcaaactcg gcaaactgga
    10381 taagggtccc gaaggcgtgt ttcgggtgga tgtacatctc cttccaattg ggaaagggtt
    10441 ccgccagacc gaaggtgggg atcccgtttt cttccagctt gcggtgaacc tcctggatgt
    10501 cattcacttg aatggtaatg tggtgcggcc cttccccccg attgtcaagg aatcgttgca
    10561 caaaactatc cttttccagg ggatcgatga gctcgatgaa gcaagaggtc cccagttcga
    10621 tggtggtcca gcggaacttc tgccagggta ccggggcctc gaaaagattc ttccctccca
    10681 ggacatcgat gaaaaaagct ttggccttcg tctggtctcg aacggcaatg gaaacgtggt
    10741 cgaccctgcg aatcataatt tcctcctaat tcttggatat ccactccgcc ttttttacct
    10801 agcatggttt gataatgaag tgcaagttct tggttggtcg ttcctcgttc cttgttgcta
    10861 gttttcggtt cgcgggaaat ccctacaacc agtaatccaa accgattccc gcgaaaccta
    10921 ttttatggtt gacccccttc gggaattata ctatcttagt aagtgatcac ttaataacgg
    10981 gcgaaagggg aaaggcatgg gaaatcgaat gacggcagag gaccgcaggg ggcagatctt
    11041 gcgcggggcc atggagctct ttgccgaaaa aggtttccgc ggggccacta cccgggaaat
    11101 ggcccagcac ctgggaatca gcgaagccct catgttcaaa tactttccca gcaaggaggc
    11161 gctctaccgg gcgatcatcc aaaaaagaac cgatggggcg gaagatgtgt tcttccccca
    11221 agaagccgtc caggccaaag atgaccggca ggtgttccaa tcgattgcct ccaacctgat
    11281 tgccaggaac actgaggacc ccaccttcat gcggctcatt ctctacagcg ccctggaggg
    11341 ccacgaactt tcccgcatct ttttcgaaac ccatgtcatg ggacaaactc gtctattgtc
    11401 ggactatatc cgccagcgga tcaaggaaaa ggcgttcaag ccggtccacc cgcttctggc
    11461 ggccagggcc ttcatgggaa tgatcattca ttatgtggaa ttccaggaaa tttatggaat
    11521 gaaaggcttt ctcaaattct ctcagaaaaa agcggtgcat accttcgtgg acatgttcct
    11581 gaatggatta accggaaaca agaaaaaaaa tggagcctaa aagcgaaaag ccaactccta
    11641 accgccgtaa aaagagggtc atgatcctcg taggagtcgt ctgcctcggg atcgcgcttt
    11701 attttggggt ccagtggctt cttttccgct ggcattacgt ctccaccgat gatgcccagg
    11761 tgaaaggaaa tctggtcaac ctcagcgcca aagtatccgg gagaattgtg cagatgcggg
    11821 tggaagaagg ggacccggtc caagtcggcc aggttctcgt ggagatcgac cctaaagact
    11881 accaagcctc ccaggcacag gcccaggcta acctggaaat ggcgcgccat gatttggcta
    11941 aagcgattac ccagctctct ctaaccaaag aacgggtcat tcagggtatt ggcacggcgg
    12001 aaacatcttt acgggaggcg agagaaggcc tgaaatttgc cgaagaagat gctgctcttc
    12061 agacggaccg ggtcaataag gacattgacc gcgcccgggc gagccttcag gcagccaggg
    12121 ccagggtggt agaagccagg gccaccatgg agaatgccaa aaaggagttc gagcgcaatc
    12181 atgagctttt ccggcaaaat tatgtggccg aaaatacccg cgatacggcc gaaacagcct
    12241 ggcaggtcgg gcagagcaaa taccaggcag ctgtggaaaa cgagcgggaa gctctctccc
    12301 agttggagtt ggcagaagcc aatcagcgtt cgattgacct gaaaaaacag agtatccgca
    12361 tcgctgagca gtctctggag agggccaaga ttaatctggc tttggctcag gaggagaagc
    12421 agcagataac cctccaggaa aaaagtattg aattcttgaa agcgaaggtc caagagacag
    12481 aagcggctct tcaactggcc gaccttcgcc ttcaggagac caagatccag agcccgatct
    12541 ctgggacggt atccaagcgt ctggcggacc agggccaggt cgtgcaggtg ggccagccgc
    12601 ttctcgtcgt gaacaatccg ggggaaaaat gggttgtggc caatgtggaa gaaacgaagg
    12661 tccggagggt ccacccgggg gccaaagtaa ccgtagaggt ggatgctttt ccggggaatg
    12721 cctttgaagg gcaggttgaa ttcatcgggt ctgccgcttc ctctgaattt gccctgcttc
    12781 cgtcggagaa cccgtcgggg aatttcatta aagtcaccca tcgaattccc gtaagggttt
    12841 cggtaaaaga cccgaagaac aagctcaagc ccgggatgat ggtggtggtg gcaattgaag
    12901 ccaaataaac aagtttccgg tttcaagttg caggattcag gtaaaaatta gcctttatag
    12961 ctttcatccc tgaagataaa aaagccgtga tttgaaatcc ttgccacaat tgtcgttttg
    13021 ctaaaattct gtttccagga tcttttaacc aaagggaatt attataaaat taataacagg
    13081 ccttggcggg attgtcggaa aaggaaactt tgcttgccag cgaatcgggg cctaattgtt
    13141 caaaatttaa attaatttcc actgtttaga atcaattggc ataaaggttg ctttttgata
    13201 ggcaagttcc gcaaatttta gcgtaaattt ccagacttca ttcaaggaat cggtccgagt
    13261 gaacaacttt tcccaccaag gcaaggatca taagtgggga gtcctgggag ttgtggtcct
    13321 gggcagcttt atggcgatcc tggataataa catcgtcaat gttgctcttc ccaagatgat
    13381 ggcgaatttc ggggccacgg tcgatcagat cgaatgggtg gtcaccggct atatgatcgc
    13441 tttcgccatt tccatgccaa ccaccaactg gctgaaagaa atgattggtt tgaaaaaatt
    13501 gtttattttc agcctggtct ttttttgcct gggatcagct ctctgcggca tggcctggga
    13561 caaagattcc ctgattggct tccgggtcct tcaggccctc ggaggcggag cgttgatgcc
    13621 aacgggtttg actctggttt cggaagcctt tccgccccag gaacggggga tggccatggg
    13681 aacctggtct attggagcga tggtcgcccc cgcagcaggt ccttttttgg ggggctatct
    13741 ggtggacgag gtcagttggc ggtcgatctt ctacatcaac ctgccggtag gggctgtggc
    13801 catcctgatg gcactttgga tccttccctc ggtccggccg gccaacccat cccgtaggtt
    13861 ggacctgacc ggtttcatca gtttatccgt cttcctcgct ttcttcttga tcgccttggc
    13921 ccaaggccag cgtgaaggat gggattctga ctacattatc gcctgcctta gcttaagcgt
    13981 tgtggggttg gtggcattcg tcgcttccgg attcctggtg aaagacccga tcattgattt
    14041 gcatcttttc gccaaccata attttgtgat ggccaatatc attaacttag cccgggccgt
    14101 ggccatcttc ggggccatgt tcctactccc ccttttcctg caaaatgttt tggactacaa
    14161 agccctgcat acgggaatta ttttagcccc cactgccata agcgtagcca ttgtctctcc
    14221 cttctccgga ctcatatccg accgaattgg cccccggatc cccttgttct gcgggactgt
    14281 gctggtcgcc ttttcccttt acatgtataa agacctctca ttggactccg attactggtt
    14341 tctattctgg ccccaggtga tccggggagc ggggatggga ttgctgaatg cccccctcat
    14401 gagtgccgct ctcaatgcgg tccgccggga gcagaccagc atcgcctcaa gcctcttgac
    14461 cgtcaccata caggtcgggg gggccatagg ggtggccctt ttagcaacca ccctggaacg
    14521 gcgccagttt ttccattaca cccattttct ggagcatatc aacaatgcct caacccccat
    14581 ggtgatccag gcccaatccg ccatggaggc gttgcttgcc cgggcgggat atgccccggc
    14641 tgattttcta ggaaaaggaa aaaccctgct ggcgttgtgg atgcaaaaac aggctgcggt
    14701 gggcgcattt gaggatgcct ttgttctcgc aggactactc attacggttg gtttcattcc
    14761 agccttgctg atccggaatc gccatggttc ccaaggttca aagccactca gcataggcga
    14821 ataaggttga attttgggga attgtcgact aaattgggga tctattttgc gctggaaaaa
    14881 attgtccagc agaagaagcg agcttgcccg aggaaagggg attgaaggat gaaaaaaatg
    14941 gcgacgaagg gaatgacggt ctgggcactc aacctgatct ttcttttatt ctggggcggc
    15001 tgggctctgg ctcaagaagg tcttatcccg cccccgggaa agcctttgac cttggaccag
    15061 tgtgtggcca tcgcggtgaa gatccatccc gccctggccg ccaatcaggc cacggtggag
    15121 gcccaaaagg ccctggtcga gcaagccctg gccgcttact atccccagtt aaattacaat
    15181 accagctatt ccgcctttac caccaacttc agcacctccc ccattagcaa tcccggcgga
    15241 ggcgcgatcg gcgtacctac taccgggccc aaccggtacc gctgggccta cacggatgtc
    15301 gtttctaccg ggcccacggc aacccagacg atatgggatt tcggccgcac cccaaataac
    15361 gtgcggatca accgggagaa cgtcaaggcc agcctggagg acctcatcac caccaagcag
    15421 acggttatcc tgaatgttca gacggcgtat tatggtctgc tccagacgca atacctgatc
    15481 gatgtggccc aggataccgt caatcaaacc cagctgcacc tggaccaggc gcagggtttt
    15541 taccaggcgg ggacccagcc gaagatttat gtgaccaacg cggaggtggc tctggccaac
    15601 gcccagttgg ccttgatcca ggtcaagaat aattcctcgg tggcccgggt gacgttgaac
    15661 aacgccatgg ggctgcggca ggatttgaat tttgagattg agaagaagat cgatattgcc
    15721 cccaaggtta cgcctttgga agagatcctg caaacggcct atgaaaagcg gcccgagatt
    15781 ttgaaactga aagctcagca gagcagccag gaagcggcca tcaaattagc caaggacggt
    15841 tattatccca ttctttcggg caatgcgagc cttctgtacc ggagcacggc attggacttg
    15901 agccaacctt tctactggga ctcttttttc tcggccactc tgagtattcc cctttttagc
    15961 ggattcctga cgcccaacca ggtgtcgcag cagcgggctg ttctgatgaa tttgaagtcg
    16021 caggaggaga gcacgcggca gaatattcgg ctggaggcgg agcaggctta tctcagcctc
    16081 aaggtggcca tcgaccagat ggcggtgacg caaaagacgg tggagcaggc caaggagaat
    16141 tacgatttgg ccagcgggcg gtaccaggtt ggggtggggt ccccactgga aattaccgat
    16201 gccgaagttt ccctggccaa tgcccgggcc aattacatcc aggccgttta cacctttaaa
    16261 accgcggaag cgcggattga aaaagccatg ggcataaacc gataagccgg gtaaggcata
    16321 aattgtttga aggttctgag gggaaaatag tttctcccca aaccgtaacc attagaagat
    16381 gagaaggaaa tgaaaaagac aatcatcgtg ttagcgatct tggcggtggc ggggctggga
    16441 gccttccggg cggcccaggt catttccgcc cgaaagactg aggacccgaa gcgccagggc
    16501 atgggccagg tccctttagt ggaagtcgcc ccggtgaccc aagggttgat tgaggagaaa
    16561 atgcttcgaa ccggagatat cgctcccttc gcccaggtag tcatctattc caaagtacag
    16621 gggtgggtgg agaagattaa tttccgggaa ggggatcggg tgagggccgg gcaggttctg
    16681 gccaccctgg atgctcgaga agccgaggcc acggtggccc aatccaaggc cagcctggag
    16741 gcggcggtcg cccgtttgaa acaggtaaaa gcgacctcgg aggaaaccat ccaatctcag
    16801 atccagcaag ccaaagcgaa cctggatttc gccgcggagg acctgaaacg cttccaggag
    16861 ctccaccaaa aggatttcat tgcgcggcag cagctggacg atgcccggac caagtacagc
    16921 gtggccaaag caaattacga ccttgctctg aacaacgtcc ggcagaagac ctgggagaac
    16981 gacatcgccc tggcggaggc gcaagttcag caggccaaag ctaccctgga cctgaatcag
    17041 gcccagctcg ccaacttgat tattctggcg cccatgaatg gaaagataac caaacgctac
    17101 gtcgacccgg ggattatggt gaaagacacg acccccatcc tggccatgat ggacctggat
    17161 gaggttaaaa tgttggtgaa tgtgattgaa aaagaattgg ttcgtttgca aaaggggcag
    17221 gaagtcaagg taacggttac ggccttcccc gaccgtacgt tccaggggcg aattgcgatc
    17281 atcacccccg cgctcgacct gcaatcccgg accgcagaaa ttcagatcac catacccaac
    17341 cccggatata ttttgaaccc cggcatgttc gggagggtgg aggcgcttct ccgggctgat
    17401 ccgaaggcca cgttgatccc gatccaggct ttgattacgg aagaagacaa ggattttgta
    17461 ttcgccctga aggatggcaa agtctaccgc cgccccgttc agaaagggat ctccaaagac
    17521 accgtcgtgg aaatcatcca aggtctgaat cccggggatc aagtagttac agccgggcag
    17581 gagttcctgc gtgacggaat cccggtgcgc ctggcggcca aatctgagaa gaaatagatt
    17641 taaggataga aaacatgaac cttgcaagat tcgccctgcg caacccgatc actattttaa
    17701 tggcggtttt gatggccatc atcctgggcg tcatctcctt ttcgaggatg cccgtggaca
    17761 tgtttccgga gatcaccttt ccctccatca ccgtcgccac tttttacaag ggcgcgaacc
    17821 cacaggacat cgagcggatg gtcacctacc cggtggaaaa agccgtttcc accgtaaacg
    17881 gagttaagta cgtccagtcc atctcccgtc agggtgcctc cctggttacg atctatttca
    17941 actggggaac gaacctggac agcgctcagt ctgatgtgtt gcaagtggtc aacctggccc
    18001 gcgctgacct gcccaaggaa gttgaaaatc cgatcatccg aaaattcgac gtctcccaga
    18061 tctccgtcgt cttcatcacc ctcatgggcg ggggcctcaa cgagggccag ctctatgacc
    18121 tggcctacaa cgtcgtcgag ccggagatcg agcatgtaac caacgtcgcc tcggcccggg
    18181 tcgtcggggg aatggtccgg gaaatttatg tgcacttcta ccgggagcgc ctgcaggcgt
    18241 ttaacctgtc tcccgacgat gtcatccagg cggttcagca gtccaaccta attctacccg
    18301 cgggggatat caaagccgga ccttatgatt accgcctttc tacggagacg cagttcagtc
    18361 tggtaaagcc catggaaaat atcatcgtct ccaacttcca gaagcggccg gtctacgtcc
    18421 gggacgtggg agaagtgaag gactattacc aggatcagac caacctcatc cgggtaaacg
    18481 gccagccggc cgtggccttg tccgtgcaga agacgtccgg aaccaatacg atccaggtgg
    18541 tggatgacgt ccgcaaggct ctgccaagaa ttaagaaact gcttccgccg ggagtggtga
    18601 tgttggagct cttcgatcaa tccctctaca ttcggaactc catcaagagc ctgcagcacg
    18661 aagcgctcat gggagcagtc ctggccatcc tggtgatcct ggtttttctg cgcaacctgc
    18721 gcagtacgat gattgtctcc ctttctattc ccatctcgat cgtctgcgct ttcatcctcc
    18781 tctacttcaa caaccttact ttgaatactt ttaccctcgg gggcctcgcc ctgggagtcg
    18841 gtcgcctggt cgatgatgcc atcgtcgtgc tggagaacat ttaccgccat cgcagcgccg
    18901 gtgagtcgcc ccaggaggcg tccatcaaag gctcgggaga agtcggcctg gcggttctcg
    18961 cttctaccat caccaccatc gtcgtcttcc ttcccgtaat tttcatcacc ggcattgccc
    19021 ggctgctttt cacaccgctg gccttgacgg tggccttctc tctgatcgct tcctacttcg
    19081 tctccctgac ggtcattccg gttctctccc gcaagtacct gcatccggaa agtgaagagg
    19141 tgttgccccc caccccgact gcgctggaac ggacgaaatg gcggttcaag gttttattcg
    19201 agcggatcga ttcggggtat cagtccctcc tggcctgggt tcttggccac cggaaaactg
    19261 tggtcattgc ggtggtcgtg gctttcctgg gcagtttgcc gctttatctc tttatcggga
    19321 cggaattctt tccttccatg gatgagagcc aattccgctt catcgtccag ctccccgtgg
    19381 gcacacgcct cgaagaggcc ggccgcatgg cggcccgtat ggaagaggtc gtccgggaga
    19441 ccatcggcaa ggagattacg gcggtccagg taaatttcgg aattccgacg ggagtgtcgg
    19501 cgatctggtc acaaaacacc ggcccgcaca tggggtggat ccgggtcagg ctgatagacc
    19561 ctggagagag gggattatca tccgatgccc tgatggaaaa gctccgccca aggctcttgc
    19621 aggaattccc gggggtgcgc atcttcttcg tcgccggggg aatcgtgcgc atgctcattt
    19681 ccttcggcta tgacaacccg atcgatgtcg agatcctggg ctatgatttc aaggctggcc
    19741 agaagctggc ggatcaggtg ggggcgatca tccgccagac gcccggcgcc aaggacgtcc
    19801 ggatcagccg ggagccggac tatccccagc aaaacatcgt catcgaccgg gagcgggctt
    19861 ccctgctcgg gttgagcgtg gcccaagtgg cccgggcgat ccagactttc atcaatggct
    19921 atcaggcctc catcttctct gacccaggga ccgggaacca atattacatt acagtgcggg
    19981 cccatggggc cgaccgcacc tccgtcagtg acctcgacca gatttttctc tttaaccgtg
    20041 atggacggcc catctctctg gacaacatcg cgaacatttc ccgcggagtc ggacccatcc
    20101 agattgaacg caagtaccaa cagcgcgtca tccatgtgac cgccaatacc tttgggcgcg
    20161 acctggggag cgtggccgcg gacattcagg ccagtattga caaactccag ttacccccga
    20221 acttcaagat caacatgacc ggagctgtag aaagccagcg ggaatcgttc atcgccctgc
    20281 tgggagccct aatcctggcc gtggtgctcg tgtatatggt gctggcctcc cagttcaaat
    20341 cacttatcga ccccttcatc atcatgttct ccgtgcccct gggtttgatc ggggtgctct
    20401 gggcgctctt ccttacccag acaaaccttt cagtgacctc cttcatgggc gtcatcatga
    20461 tggccgggat cgtagtgagt aacggcattc ttctagtcga ctataccaac cggctgcgcg
    20521 gacggggtat gagcctggag gaggcggtca cgctcggggg gcggacgagg ctccgtccca
    20581 tcctgatgac taccctctgt acgatcctgg gcctcatccc catggccatt ggcctgggag
    20641 aaggttcgga atcaaacgcc cccatggcca tcgcggtgat cggggggctt tcggtttcca
    20701 ccctcctcac cctggtgttc atcccgaccc tgtacaccat cttcgaaagc cgttttaaga
    20761 gagaaatcca taccgaggaa aaagaacagc cgtgaaaaag aaaatcacct tttggataat
    20821 tctgatcctt ctcttggccg gcgttgccct tggttatacg tactactacc ggggaaagga
    20881 ttcggccgta aagtaccgca cggccagggt ggaaaggggg aacctctccg ctttcgtaac
    20941 ggccaacggg acgatcaacc cggtgattac ggtaacggtg ggctctcagg tttcgggcac
    21001 gatccagaag ttgtatgccg attacaattc ccaggtaaaa ttgggggaac tcgtcgccct
    21061 gatcgacccg gcgatctttc aggcccaggc ttcccaggcc aaggccaagc tggagaacgc
    21121 ccgggcaacc ttcctgaacg cgcaggccga tatttctacg gccaaagcca acgtggagtc
    21181 tagccgggcc aacgtgatta aggccaaggt ttcggtggat gatacgaaac ggagtctggg
    21241 ccgttccctg gagcttttca gccgcaacct catctctgcc agcgaccggg atgcttccca
    21301 aacggcctat gattcggccg tggccctgtc ggaggcagcg caggcccagc tgagcgccag
    21361 cgaagcgcag ttggaatcgg cccgggcgaa gctggaagcg gcccgggcgc aggtcaagca
    21421 gtcggaggcg gagctggagc tggcccaggt gaacctggat cactgcaaaa ttaccgcgcc
    21481 ggtgacgggg attgtcatct cgcgcaacgt ggacgtgggg cagaccgtgg ccgcgagttt
    21541 acaggcccca acccttttta ccatcgctca ggaccttact gagatgcaag tggatactaa
    21601 tgtaagcgag gcggacatcg ggcgggtggc catcggccag gaagcgacgt ttaccgtcga
    21661 tgccttcccc aacctaacct tcctgggaaa ggtaaccgac atccgcaacg cccctgtaac
    21721 cgttcagaac gtggtcactt atgacgtcgt gatcctggta aagaacccgg agctgaagct
    21781 gaggccggga atgacggcca acgcctcgat cctggtggcc cagagtgaga atgccctgaa
    21841 aatccccaac tcggccttgc gtttccggcc ggattttgcc agaaaggaag ccgaagcctc
    21901 cccgaaaggg caggtttcac cttccccccc tcccccttcg gcaactagcc cggaacagat
    21961 cttggagcgt ttgaaagccg agctcaagct aaccccgcaa caggaggcag gcattttaag
    22021 catcttgaag gatgcccagg gagagattct ggcggcgcga aaagccgggg gttcggaaga
    22081 ggccaaaaac aaggccaaag agctccgggc ggccaaccgg atcaagattc gttctttgct
    22141 tacggaagaa cagaagaaga aatatgatca gttggaccgg cgaccggatt cctcccaggg
    22201 tgcgacccca atgtataggg tttggctggc cgttccggag gggaggcctg tgccggttga
    22261 catcaccacg gggatatcgg atggttccta tacggaggtg acctccggtg ccctgaagga
    22321 ggggcaggaa atcatccttg aagccatggc cgggaacggc aagagcggag gctcctcgca
    22381 ggcccctcag ccctccatga aaggcttccg ataaacctgt tgcgggtttc aggtttcaag
    22441 tttcaggttc aaaaatgcaa ccctccatga atctccaacc ggtaatgaag aaatcaattg
    22501 ctgttaactc gaaacacgaa actatcttat gagtgaattg atccgcgtcg agcatctgac
    22561 caaaatctac tacctggggg aggtgcaggt gccagccctc aaggggatgg acctgaccat
    22621 tgagcccggt gagtgcgtag ccatcatggg ggcgtcggga tcgggtaagt ctacctttat
    22681 gaac
//
LOCUS       DASPRR010000462        12519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1560055, whole genome shotgun sequence.
ACCESSION   DASPRR010000462 DASPRR010000000
VERSION     DASPRR010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12519)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12519
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560055"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1427)
                     /locus_tag="VEL68_16970"
     CDS             complement(333..1427)
                     /locus_tag="VEL68_16970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ37718.1"
                     /translation="MPSAPLVSVVMAVFDGERYIRDAIDSILGQSFRDFEFIIINDGS
                     TDNTAGILQQYAQLDERLLVFRQENRGLIAALNRGCGLARGKYIARLDADDISVPDRF
                     EKQVDFLDRHPAVAVLGGAIKLINPKGALIGEWRYPVDDRQIKEELLRTNCLCHPAIM
                     MRKDAFDATEGYRRPFRHAEDYDLWLRMAERFELANLPDILLYYRVHAGQVSPKNLSQ
                     QVFSALAAEAAARIRRDTGQDPSLPLDGVNSDSLVSLAVSRDRLTQALITRHLSWAGQ
                     MREIGEYRLAEKLLRKALVLSRTEATRDWLADIHVEFARCQYLRRSPVGALISLAKAA
                     ALNPTLAPSFLRYAWLAVRRFGSQSPHPED"
     gene            1807..2427
                     /locus_tag="VEL68_16975"
     CDS             1807..2427
                     /locus_tag="VEL68_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXZ37719.1"
                     /translation="MGDILTTALVGISTLGMAIVTGTRNKEPRFPEIKRDQMNDAQKR
                     VYDEIATSRGGVRGPFGVLLRSPDLADRWQRLGEYVRFKTSLPPRLNEFAILITARFW
                     GSKYEWFAHRPLAIKAGLTESIAEDLAQNKRPANMRPDEELVYEFCTTLHRQHFVGDP
                     LFKSAMETLGERGVIDLVAVSGFYVAVSMVLNVAEIAIPPGEKSPW"
     gene            complement(2492..3829)
                     /gene="der"
                     /locus_tag="VEL68_16980"
     CDS             complement(2492..3829)
                     /gene="der"
                     /locus_tag="VEL68_16980"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXZ37720.1"
                     /translation="MKPIIAIVGRPNVGKSTFFNRLVGQRKAIVEDEPGVTRDRNYAE
                     ASYEDHPFILIDTGGFEPTSSDRIQQQIKEQVEVAIQEADLIVFLMDGKEGLNPTDEE
                     IAGYLRKVTKPTFYVVNKVDGEKQEDSVPDFYHLGVSPIYSISAEHGRGVGELMDEVI
                     RVLPPGSSEPERIEEGIRVAIVGRPNVGKSSLLNKLIGRPRAIVDSTPGTTRDTLDTP
                     FLREGRRYVFIDTAGIRRKSKVTERVEKFSAIKALRSLERCDVALVLIDGFEGLAEQD
                     ARIAEFAEESGRAIILVVNKWDLVQKDTSTMEEYKERIRREVKTLDYVPILFISALTG
                     QRASRIFQTIDQVTAEHRKRIPTGELNHWLREAVQSYPPPPFRNRPVKLLFISQVSTA
                     PPTFVLFANEPMGLGESYQRYLLRRLREKHGFTGTPLRLYLRKRKKEKAKSGG"
     gene            complement(3904..4443)
                     /locus_tag="VEL68_16985"
     CDS             complement(3904..4443)
                     /locus_tag="VEL68_16985"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXZ37721.1"
                     /translation="MKRFFALSAIGKDRPGIVAEVSGLIYECGGNLEDSRMTRLGEQF
                     ALLILLSGSGEEFQYRLSTGCKRLEWEKHLSIFLTPLEAFEGKPGEKGRIELYELSTT
                     GMDRMGIVYRVSRLLADQRINIADMQTKATPSPESGTPIFTMKVLLEIPADISAKELL
                     EKLNRLGEELAIDISLKKS"
     gene            complement(4576..5538)
                     /locus_tag="VEL68_16990"
     CDS             complement(4576..5538)
                     /locus_tag="VEL68_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HXZ37722.1"
                     /translation="MIEVLKPGLFTTIQDLGRWGYQSFGVGIAGALDPFALSAANLLV
                     GNPEGAAGLEITLQGLSLRFHRESAVAISGADLGPRLDGLSVPTWTSVLAPSGSTLSF
                     KGRNSGVRAYLAVSGGIDVPQVMGSRSTYLLGKFGGLEGRALKAQDLLPVAPQPVNFR
                     EWVGRSFPEEMRPLYLKNPTLRAVLGPFDEFFSEESKKAFLSTPYTITAQSDRMGYRL
                     QGTQITRQRKEELITCGLAFGTVQVPPNGQPILLLVDRQTIGGYPIIATMIHADLPLV
                     AQSAPGDTLRFSAVSPDEGRQAYIRLWGNLDKFKHSAISDQRSA"
     gene            complement(5602..6309)
                     /gene="pxpB"
                     /locus_tag="VEL68_16995"
     CDS             complement(5602..6309)
                     /gene="pxpB"
                     /locus_tag="VEL68_16995"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HXZ37723.1"
                     /translation="MGEKIRIRPAGDQALLVEFGTEISPEINLRVQEFSHRAAVQKIP
                     GIGEIIPSYCSVLVYYDPILLSFADTVSWAKEFLDSKPLETQASAPIKEVPVLYGGEY
                     GPDLSFVAAHNQTSEEEVIRLHTGQTYLVYVVGFSPGFAAMGVVPPKVQAPRLASPRT
                     KVPAGSVGIGGLQTGIYAVESPGGWRLIGRTPLNLFDLQKNPPSFFQAGDYARFYAID
                     GKEYKKRIQESGTRIQD"
     gene            complement(6313..7077)
                     /locus_tag="VEL68_17000"
     CDS             complement(6313..7077)
                     /locus_tag="VEL68_17000"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HXZ37724.1"
                     /translation="MPDRVDINSDMGESFGAYKLGRDAEVMEYISSANIACGWHAGDP
                     MVMEQTVRMAKERGVGVGAHPGYPDLMGFGRRRLDLSISEIENYMLYQMGALYAFART
                     HGLPLQHVKAHGALGNLAFVDLEVSKAIARAALRFSKDMIFVALTGTVMVKAAKEVGV
                     RYVEEVYADRVYNPDGTLQSRKIAGSVIHDPEKAAKQALTIMKEGYVLAHDGTKVKVK
                     PETLCVHGDTPTAISILQKIWEELKKASIAVKPMGL"
     gene            complement(7227..8210)
                     /locus_tag="VEL68_17005"
     CDS             complement(7227..8210)
                     /locus_tag="VEL68_17005"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HXZ37725.1"
                     /translation="MDANGNLVEVKDLKKFFPVRAHFLSRSKTFVQAVTGVNLTIRRG
                     ETLGLVGESGCGKSTLGRLILRLEEPTEGHIFFEGDDILEYDSERLRQLRRQMQIIFQ
                     DPYSSLNPRKTVGSIIEEPLIIHKIGTRKERQERVLKLIEVVGLRPEHINRYPHEFSG
                     GQRQRIGIARALALNPKLIIADEPVSALDVSIQAQVLNLLEDLQDEFHLTYLFIAHDL
                     HVVEHISDRVAVMHLGRIAELAMSDDLYRDPKHPYTQALLSANPIPDPTLERKRIILQ
                     GEVPSPINPPPGCNFHTRCPYKFEPCPVEIPPLLEIEPNHWVACHLHGPKK"
     gene            complement(8268..9257)
                     /locus_tag="VEL68_17010"
     CDS             complement(8268..9257)
                     /locus_tag="VEL68_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37726.1"
                     /translation="MAGANHFLLTIRNLHTYFFTDEGVAKAVDGVDLELKEGGTLGVV
                     GESGCGKSVTALSVMRLIPDPPGKITQGEILFAGTNLLDLSEAEMRKIRGRSISMIFQ
                     EPMTSLNPVFQIGDQISEVLRLHEGLSRREAWDRSIEMLKLVGIPSPERRVQEYPHQL
                     SGGMRQRAMIAMALACSPKLMIADEPTTALDVTIQAQILELINRLQREKGMSVILITH
                     NLGVIAETAQKVAVMYAGRIVEYAEVKPIFGNPMHPYTQGLLKSIPRLDEDHARKARL
                     EAIPGLVPSLLELPKGCKFSNRCKYVFEKCGEEPQLIEAEPGHLVRCWLHGKK"
     gene            complement(9474..10280)
                     /locus_tag="VEL68_17015"
     CDS             complement(9474..10280)
                     /locus_tag="VEL68_17015"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HXZ37727.1"
                     /translation="MEKLYLYQWTWAEVKAYLKKDSVIIVPFGSTEQHGLHLPLGNDA
                     LVAIRLAEDAARITKTIVAPPCWYGWSPHHMAYPGTISITPETLISLLYEVLRSLIYH
                     GFERLIVINGHRTANLPPMQIATSRIRNETGAYVTIADPFYIGDTMSRKIRESPPGGI
                     GHAEELETSHMMHIFPELVDIKKAVKNLPHMKKFHFADPYVIADRVFTPSSLESFREA
                     TKPSGVTGDPTLSTAEKGKRLHKALVDNLVELIKLARKEKVALKPVTPCF"
     gene            complement(10401..11294)
                     /locus_tag="VEL68_17020"
     CDS             complement(10401..11294)
                     /locus_tag="VEL68_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008227191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37728.1"
                     /translation="MSAKQPFNPFPQEEARRSSPVFIWRRHFKNKAGVLGLILLALVL
                     LCAFFGPLLYPMNPFELLGRKFIPPGETFFLGTDYLGRDVLAGIIQGSRTSLLVGIMA
                     AGMMMTIGIAFGALAGYYGGLIDEALMRLTEFFQVLPSFILAMVVVTLFKPTLWTIIL
                     AISISTWTGTARILRAEFMSIRERDFVTASRALGSKNKRIIFREILPNALPPMIVNAS
                     LMVGVAILFEAGLSFLGLADPNVMSWGYMIGSSRVYLRQAWWTVTFPGLAIFITVLSL
                     SLVGDWLNDFLNPKSKGRKQG"
     gene            complement(11308..12291)
                     /locus_tag="VEL68_17025"
     CDS             complement(11308..12291)
                     /locus_tag="VEL68_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37729.1"
                     /translation="MPSSSFGWYLLRRILQAIPVVVGVVILNFTLLHLAPGDPAMVFV
                     GEAGGATEEILKEVRRQFGLDKSLMEQLFIYGEKVLLGDLGFSYFQGKPVMDLILEKI
                     PATILLILTAIFLAVTLGVLTGVIVAQRPTSLGSSFITVLSLFGFAMPVFWLGIMLIL
                     AFSLYLPLFPGHGMITVGLTGSFWDLAKDVLHHLVLPAFTLAIIYIAQYSRLSRASML
                     EVIGSDYIRTARAKGLSERLVIYKHALRNAILPVLTVFGIQIGHLLAGAILVETVFTW
                     PGMGRLMFDGILRRDYPLIMGILIFSSVLVILANLITDLLYGVLDPRIRRR"
     gene            complement(12324..>12519)
                     /locus_tag="VEL68_17030"
     CDS             complement(12324..>12519)
                     /locus_tag="VEL68_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37730.1"
                     /translation="QNFAKRKNLYHKVQEILMDELPLIWIQEGGLYTFYNKDFDGIPM
                     DVWGVLNPYDTVYWRKGKTG"
BASE COUNT         2724 a         3441 c         3629 g         2725 t
ORIGIN      
        1 cgattcgtaa gttcgagatc gatcactctc ggcgcctgcg ttcgaaagcc gggcaaaatg
       61 ctggggagga gtcgaaccaa gagacggaga tcggcaaaga tggcggctac tgggtggggg
      121 gatttacgca aagccattag ggtccttccc aaaaacgccc gctcttagca cttcgcctcg
      181 gaggtatgcc ccagccgcct cgaaaactct atccgcgacc gagcggcaac cgaggagtgg
      241 agccttgtcg aagcaaggcg gggctgtcaa cccgaagccg gtttaaccag gcgcgctgga
      301 agaaggcgcc aggagatccc ggagttttac atctaatctt cgggatgtgg agattgagac
      361 ccgaatctac ggactgccag ccaggcatag cgtaggaagc tgggggccag ggtcgggttg
      421 agagccgccg ctttcgccaa agaaatcaag gcacccaccg ggctccgtcg caggtattgg
      481 catcgcgcga actcaacatg gatatcggca agccaatcac gagtggcttc cgtgcgggac
      541 agaaccaatg ccttgcgtag gagcttctct gcaagcctgt actctcctat ttctctcatc
      601 tgtcctgccc aactcaagtg ccgcgtgatc aacgcctgag tcaggcgatc gcgactcacg
      661 gcgaggctca cgagggagtc tgaattcacg ccatccaaag gcaaggacgg gtcctgtccc
      721 gtgtcccggc gaattcgagc agcggcctcc gcagccaagg cggagaatac ctgctggctg
      781 agatttttcg gcgacacctg acctgcatgg accctgtaat acaggaggat atccggcagg
      841 tttgccagct cgaagcgctc ggccatccgg agccagaggt catagtcttc agcgtgccgg
      901 aagggtcttc ggtagccctc tgtggcatcg aaggcgtcct tgcgcatcat gatcgcggga
      961 tggcagaggc agttggttcg gagcaactcc tctttgattt gccgatcgtc aaccgggtag
     1021 cgccattcac caattaacgc tcccttcggg ttgatcagct tgatcgctcc gcctaggacc
     1081 gccaccgcgg gatgacgatc gaggaagtcc acctgctttt cgaaacggtc gggaacacta
     1141 atatcgtccg catccaaccg cgcgatgtat ttcccccgag ccagtccaca tccccggtta
     1201 agagcggcaa tcaagccgcg gttctcttgc cgaaagacga ggagacgttc atcgagttgg
     1261 gcatattgct gaaggattcc ggctgtgtta tcggtggaac cgtcattgat aatgatgaac
     1321 tcaaagtccc gaaaactctg gccgaggata ctgtcaatcg cgtccctgat gtacctttcg
     1381 ccgtcaaaaa cggccataac gacactcacc agaggagccg acggcatttc gcgtacccca
     1441 cttttttttt gcagcttaca ccggcctccg agtggaagcc acggcaagga cgatcgctac
     1501 cccaagggtt gcgcaccagg aagatcagac gctactggtt cgtcgaggtg gtgataagta
     1561 ggcgggttgt ctctatccga gcgcattcgc atacggttac ggcccgccga cggctcgaac
     1621 acgtcaccga gccgattgat aaatactcgc ttggcgatgg gttgatcata caaaaaaggc
     1681 agtggtgtgt caaggctaat tgcccaccat cctgctagcc cttgacatgc aatctccttc
     1741 tctcgtacac tcgaacccaa cgaataaggg ctacatgaaa aatcgaagcg gagggagaaa
     1801 aggttcatgg gggacattct gaccactgca ttggtgggca tttctaccct gggaatggca
     1861 atcgtgactg gtactcgaaa caaggaacct cgctttccgg agatcaagcg ggaccagatg
     1921 aacgacgccc aaaagcgagt gtatgacgaa atcgccacct cgcgcggggg ggtgcggggc
     1981 cccttcggtg tcctgctgcg tagtccggat ttagccgatc gatggcaaag gctcggcgag
     2041 tatgtgcggt ttaaaacctc gctgccgccg cggctcaatg aattcgcgat cctgatcacc
     2101 gcccgttttt gggggtccaa gtacgagtgg tttgcccaca ggccgctggc gatcaaggcc
     2161 gggcttaccg aatccatcgc cgaggacctg gcgcagaaca agcggccggc gaacatgagg
     2221 ccggacgagg aactggtcta tgaattctgc acgaccctgc accggcagca tttcgtcggc
     2281 gacccgctgt tcaaaagcgc gatggagaca cttggggaga gaggcgtgat cgacttggtc
     2341 gcggtcagcg gattctacgt ggccgtttcc atggtgttaa atgtggccga aatcgcgatc
     2401 cccccgggcg agaaatcgcc ctggtgattc ttccccggcc gatttcggcg ccgctcgcgt
     2461 cggtcccccc tcgcttctta gcggggcgac gctagcctcc gcttttggcc ttttctttct
     2521 ttcgcttcct caaatagagc cgcagcgggg tgccggtgaa gccgtgcttt tcccggagtc
     2581 ggcggagaag gtagcgctgg taggattctc ccaggcccat tggttcgttc gcgaagagaa
     2641 cgaacgtcgg gggggctgtc gagacctggg aaatgaagag cagtttcacc gggcggttgc
     2701 ggaatggggg aggcgggtaa ctttgcaccg cttccctcag ccagtggttc aattctcccg
     2761 tgggaatccg cttgcggtgc tcggcggtca cttggtcaat ggtttggaag atgcggctcg
     2821 cgcgctgacc ggtcagggcg gagatgaata ggatgggcac gtaatccagg gttttcactt
     2881 cccgccggat cctctctttg tattcctcca tcgtcgaagt atccttctgg accaagtccc
     2941 acttgttgac caccaggata attgcccgcc cgctctcttc ggcgaattcc gcgatccggg
     3001 cgtcctgctc ggctaggccc tcaaagccgt cgatcaggac caaagccaca tcgcaacgct
     3061 ccaggcttct cagcgccttg atggccgaaa atttctccac ccgctcggtc accttgcttt
     3121 tccggcggat ccccgccgta tcgatgaaaa catacctgcg cccttcgcgg aggaaagggg
     3181 tgtccagggt atcccgcgtg gtcccgggag tcgaatccac gatggcccgg gggcggccga
     3241 tgagtttgtt cagcagggaa gatttcccaa cgttaggccg tcccacgatt gccacccgga
     3301 ttccttcttc aatcctctcc ggctctgacg agcccggggg gagaaccctg attacttcgt
     3361 ccatcagctc cccaacaccg cgcccgtgtt cggcggagat ggagtagata ggggaaactc
     3421 ccaggtggta gaaatccggg accgaatctt cctgcttctc cccgtcaacc ttgttcacga
     3481 cgtagaaggt gggtttggtg acttttcgca ggtagccggc gatttcttcg tcggtaggat
     3541 ttaatccttc tttcccgtcc atgaggaaga cgatcaggtc ggcctcctga atcgccactt
     3601 ccacctgttc cttgatctgc tgctggatac ggtcgctgga ggtgggttca aaaccgccgg
     3661 tgtcaatcaa gataaagggg tggtcttcat acgaggcttc cgcgtaattg cggtcccggg
     3721 ttaccccggg ttcgtcctcc acgatggctt tgcgctggcc gaccaaacgg ttgaagaaag
     3781 tcgatttgcc tacgttcggc cggcctacga tggcgataat cggtttcata tggaaaacgg
     3841 gtaaagggtg aggggtaagt ggttaggaac aaaacgcggc catacccgct gtcaacctac
     3901 gactcacgat ttcttcaggc taatatcgat ggccagttct tcccccaggc ggttgagttt
     3961 ttccaggagt tccttggcgg aaatatctgc aggaatttcc agtaggacct tcatggtgaa
     4021 gatgggggtt ccgctttccg gggaaggggt cgccttggtc tgcatgtcgg caatgttgat
     4081 cctttggtcg gccagaagcc gcgagacccg gtagacaatt cccatccggt ccattccggt
     4141 ggtggaaagc tcgtacaatt cgatgcgtcc cttttctccc ggctttccct cgaaggcttc
     4201 caggggggtg agaaagatgg agaggtgctt ttcccactcc agccgcttgc agcccgtgga
     4261 aaggcggtat tggaattctt cgccgctacc ggaaaggaga atgagcaaag caaactgttc
     4321 gcccaggcgg gtcattcggg aatcttccag gttcccgccg cattcataga tcaggccgga
     4381 aacctcggcc acgatcccgg gccggtcctt gccgatggcc gaaagagcga aaaatctttt
     4441 catgaagcct cctcgaaaaa acgcggtcag cgctcagctt tcagcggtca ggaaaaaaca
     4501 taaattatta tatggcaaaa aaggaaacgg gaataggggt atagaaggtg ggggccagaa
     4561 ttcggtttct cgcttctaag ctgatcgctg atcgctgatt gctgaatgct taaatttatc
     4621 cagatttccc cagagtcgga tatacgcctg cctcccctca tcgggggaga cagccgaaaa
     4681 ccgcagtgta tccccgggag cgctctgggc aacgagggga aggtcggcat ggatcatggt
     4741 ggcgatgatc ggatacccac cgatcgtttg ccggtccacg agcagaagga tgggctggcc
     4801 gttaggaggg acctgaacgg tgccgaaggc caagccgcag gtaatcaact cctctttcct
     4861 ttggcgggtg atctgagttc cttgaaggcg gtagcccatg cggtcggatt gagcggtgat
     4921 ggtgtaggga gttgaaagga aagccttttt gctctcttca gaaaaaaatt catcaaaagg
     4981 acccagaacg gcgcgcaggg ttgggttttt aagataaaga gggcgcattt cctccggaaa
     5041 agaccttcca acccattccc gaaaatttac cggctgggga gcgacaggaa gcaggtcctg
     5101 agccttaagt gcccttccct ccaggccacc aaatttcccc agaagataag tggaccgcga
     5161 tcccatcact tggggcacgt caatgccgcc cgagacggcc aggtatgccc gaactccgct
     5221 atttcttcct ttgaagctga gcgtggaccc cgagggggcc agaacgctag tccaggtggg
     5281 gacggatagg ccatctagcc tggggcccag atcggcgccg ctgattgcca ccgctgattc
     5341 ccggtgaaac cttaaagata ggccctgcag ggtgatctcc aggccggcgg ctccttccgg
     5401 atttcccacc aagagattcg ccgcggaaag ggcaaagggg tccagcgctc cggcgattcc
     5461 cacgccgaaa ctctgatatc cccaccgtcc caggtcctgg atggtggtga agaggccggg
     5521 tttcagcact tcaatcataa tggaaacgta gggatcagaa ttcaggcgtc aggcatcagg
     5581 ataaagaaag cagaggacct cttaatcctg aatcctggtt cctgactcct gaatcctttt
     5641 tttatattct ttcccgtcga tcgcataaaa ccgggcgtag tctccggcct ggaaaaagga
     5701 aggcggattc ttctgcagat caaaaaggtt aaggggggtt cggccgatca accgccaccc
     5761 cccgggtgat tcaacggcgt agatgccggt ttgcaagccc ccgatcccta ccgaccctgc
     5821 cgggacttta gtcctcgggc tggccagccg gggagcctga accttgggcg gtacgacccc
     5881 catggccgca aatccggggg agaacccgac tacgtacacg aggtacgttt gcccggtgtg
     5941 cagccggatg acttcttctt ccgaggtctg attatgcgcg gccacaaagg aaaggtctgg
     6001 gccgtactcc cccccgtaga gaaccggcac ttctttgata ggagcggagg cctgggtctc
     6061 caagggcttg gagtccaaga attcttttgc ccaggaaacc gtatccgcaa aagagaggag
     6121 aatcggatcg taatagacca ggaccgagca ataggagggg attatctctc cgatcccggg
     6181 gatcttttga accgctgcgc gatgggaaaa ttcctgaacc cgaaggttga tctccgggct
     6241 gatctcagtc ccgaattcta ccaggagcgc ctggtctccg gcgggccgaa tcctaatttt
     6301 ttcccccatc cctcacagcc ccatgggttt aacggcgatg gaggccttct tgagttcctc
     6361 ccagatcttc tggaggatgg agatggccgt gggagtatcc ccatgcacgc agagggtttc
     6421 gggctttact ttaaccttcg tgccatcgtg ggcgaggacg taaccttctt tcatgatggt
     6481 cagagcctgt tttgcggctt tttctgggtc gtggatgacg gaaccggcga ttttccggga
     6541 ctggagggtc ccgtccggat tgtacacgcg atcggcgtaa acttcttcca cgtatcggac
     6601 cccgacttcc ttggccgcct taaccatgac ggtccccgtg agggccacaa aaatcatgtc
     6661 tttgctgaag cggagggcgg cccgggcgat ggccttggaa acctccaggt ccacgaaggc
     6721 aaggtttccc agcgcgccat gggcttttac gtgctgcaag ggcagaccgt gggtcctggc
     6781 aaacgcgtaa agcgccccca tctggtagag catgtaattc tcgatttcgc tgatggaaag
     6841 gtccagcctc ctccttccga accccatgag gtccgggtac ccggggtggg cgcccacccc
     6901 cactcccctt tccttggcca tccgcacggt ctgctccatg accatggggt cccccgcatg
     6961 ccagccgcag gcgatgttgg ctgaggagat gtattccatg acttccgcgt cccggcctag
     7021 cttgtaggcc ccgaaacttt cacccatatc gctgttgatg tcgacccgat ctggcattat
     7081 gacctccttt attatcacca cggaggcaca gagaacacag ggaagcaaaa attacttgga
     7141 tataattcct aagccaaaaa ctattttctg atttggtttc tagcttctga tctccgtgtg
     7201 ctctgtgtct ctgcggcgaa aggtccttat tttttagggc cgtggagatg gcaggccacc
     7261 cagtgattag gctcgatttc taagagggga ggaatttcaa cgggacaggg ttcaaattta
     7321 tatggacagc gggtatgaaa attacacccc ggaggaggat tgatgggact gggcacttcc
     7381 ccctgcagaa tgatccgctt ccgttccagg gttgggtccg ggatggggtt ggccgagagc
     7441 agcgcctgcg tataaggatg cttggggtct cggtaaagat cgtcgctcat agccagctcg
     7501 gcaattcgcc ccaggtgcat tacggcgacc cggtcgctga tgtgttcgac cacatggagg
     7561 tcatgggcga tgaagaggta agtcaggtgg aactcatcct gcaggtcctc caggaggttg
     7621 agcacctggg cctggatgga gacgtccagg gccgacacgg gctcatcggc gatgatcagc
     7681 ttgggattca gggccagggc ccgcgcgatt ccgattcgct gcctctgccc accgctgaac
     7741 tcgtgggggt agcggttgat gtgctccggc cgtaagccca ccacctcgat cagcttgagg
     7801 accctttcct gtcgttcctt tctggtgccg attttatgaa tgatgagcgg ttcttcgatg
     7861 atggagccga cggtcttccg ggggttcagg gaagagtagg gatcctggaa gatgatctgc
     7921 atctggcggc ggagctggcg tagtctttcg gaatcgtact cgaggatatc gtccccttcg
     7981 aagaagatat gtccctcggt gggctcctcc agacgaagga tcaagcgccc cagggtggat
     8041 ttcccgcacc cggactcccc cacgagtccc agggtctctc cccgacggat ggtcaggttg
     8101 acccctgtca cggcctggac aaaggtcttc gaccgggaga ggaaatgggc ccgtaccggg
     8161 aaaaatttct tcaggtcttt aacttctaca aggttaccgt tggcgtccat cgagactctt
     8221 gctatttttt tgctgaccgc taaaagctga cagctgtttt tttaccgcta ttttttccca
     8281 tgcaaccagc accgcaccag gtgtccgggt tctgcctcga tcaactgcgg ttcctcgccg
     8341 catttttcaa aaacatattt gcagcgattg gaaaacttgc agcctttggg gagctccagg
     8401 agcgagggaa ccaaaccggg gatagcctcc aatcgggctt tgcgagcgtg gtcctcatcc
     8461 aggcggggga tcgacttcaa aagaccctgg gtgtagggat gcatcgggtt gccaaagatc
     8521 ggtttcacct cggcatactc cacgatccgg cccgcgtaca tgaccgccac tttttgtgcc
     8581 gtctcggcga tcactcccag gttgtgggtg atcaggataa cggacatccc cttctctctc
     8641 tggaggcggt ttatcagctc caagatctgc gcctggatgg ttacatccag agctgtggtg
     8701 ggctcgtcgg cgatcatcaa cttggggctg caggctaggg ccatggcgat catggcccgc
     8761 tggcgcatgc ctccgctcaa ttggtgggga tattcctgga cccggcgttc cggggaagga
     8821 attcccacca atttgagcat ctcgatggac cggtcccagg cctctctccg ggacaatcct
     8881 tcgtgcaacc gcaagacctc ggagatttgg tcgccgatct ggaagaccgg gttcagagag
     8941 gtcatgggct cctgaaagat catggaaatg gaacggccgc ggatcttccg catctccgcc
     9001 tcggagaggt ccagcaggtt cgttccggcg aaaaggatct ctccctgggt gatctttccc
     9061 gggggatcgg ggatgaggcg catgaccgaa agggcggtga cactcttgcc gcaacctgac
     9121 tctccgacga cccccagagt gcccccttct ttcagctcca ggtccacgcc gtcgacggcc
     9181 ttggccaccc cctcatcggt gaagaaatag gtgtggaggt tgcggatggt cagaagaaaa
     9241 tgattcgcgc cagccatgga atgttttgtg ttttcgtcac cttcacttgt attttgaagg
     9301 ctttaaaata ccccagaata ttttttttgt caaacaaaac gcaacagtta aataagagct
     9361 tgtcgcgggc agcgggaata cccaacaatc cgaaatcacc ccagcactgc gcgaagtgca
     9421 gagcagccct gcacttcgta ggggtcaagg cgcattctga ttccactttt tcattaaaaa
     9481 cagggagtta ccggcttaag ggccactttc tccttgcggg ccagcttgat taattccacc
     9541 aggttgtcga ccagggcctt gtggaggcgt ttcccttttt ccgccgtgga aagtgtcggg
     9601 tctccggtaa ccccactggg ttttgtggcc tcccgaaagc tttccagcga agaaggggta
     9661 aagacccggt ccgcgatcac atagggatcg gcgaaatgga acttcttcat gtggggaagg
     9721 tttttcaccg ctttttttat gtccaccagc tctgggaaga tgtgcatcat gtgggaagtc
     9781 tccagttcct cggcgtggcc gattccccct ggaggagatt cgcgaatctt ccgcgacatg
     9841 gtatccccga tgtagaaagg atccgcaatg gtgacgtagg ctccggtttc atttcgaatc
     9901 cgggaagtag cgatttgcat cgggggcagg ttggccgtcc ggtgcccgtt gatgacgatg
     9961 agccgttcga acccgtgata gatcaggctg cgcaggactt cataaagcag gctaatcaga
    10021 gtttccgggg taatggagat cgtccccggg taggccatgt gatgggggga ccaaccgtac
    10081 cagcagggag gggccacgat tgttttggtg atccgtgccg catcctcggc caggcgaatg
    10141 gccaccagag cgtcattccc caggggaaga tgaaggccgt gctgctccgt cgaaccgaaa
    10201 gggacgatga taacgctgtc tttctttaaa taagctttga cttccgccca agtccattga
    10261 taaagataca gtttttccat aaggacccct tccttggcct ggcgcgtttt agtttctcag
    10321 gaggattcta aataaaatcc ttgccgggat caagcaatat tactgcctaa cctttttccc
    10381 tccatcatcc gcattcaaaa tcatccttgc tttcttccct tagatttcgg gttcagaaaa
    10441 tcgttaagcc agtctcccac caaactgagg ctcagaacgg tgatgaaaat agccaggccg
    10501 ggaaaggtta cggtccacca agcctgccgc agataaactc ttgatgagcc gatcatatat
    10561 ccccagctca tgacgttggg atcggccagg ccgaggaaac tgagcccggc ctcgaagaga
    10621 atggccacgc cgaccatcag agaagcattc acaatcatgg gaggcaaagc gttggggagg
    10681 atctcgcgaa agatgattct cttgtttttg gagccgaggg cccgggaggc ggtgacgaaa
    10741 tcacgttccc ttatggacat gaattcggct cgcaagatcc gcgccgtgcc ggtccaggtg
    10801 gaaatgctga tggccaggat gatggtccac agagtgggtt tgaagagggt caccaccacc
    10861 atggccagga taaaggaggg gagaacctgg aaaaattccg tgaggcgcat caaggcttca
    10921 tcgatgaggc ccccgtaata gccggccagg gcgccaaaag cgatgccgat ggtcatcatc
    10981 attccggcgg ccatgattcc gaccaggaga gaggtccggg atccctggat gatcccggct
    11041 aagacgtccc ggcccaggta atccgttcca aggaaaaatg tctctccggg agggatgaac
    11101 ttccggccca aaagctcgaa agggttcatc gggtacagca ggggtccgaa gaaggcgcac
    11161 aagagaacca aggctaaaag gatgaggccc agaactccgg ccttgttctt aaaatgtctt
    11221 cgccagataa aaaccggaga agagcgccgg gcttcttctt gcggaaatgg gttgaagggc
    11281 tgtttagcgg acatcttctt tccctcccta gcgccggcgg attcttgggt ccaagacgcc
    11341 atacaggagg tcggtgatca gattggccag gatcacgaga accgaggaaa aaatcagaat
    11401 gcccatgatc agcgggtagt cccggcgcag aattccgtcg aacatgagac ggcccattcc
    11461 cggccaggtg aagaccgttt cgactaaaat ggcgcccgct aagaggtggc caatttgaat
    11521 cccgaagacc gtgagcacgg gaaggatcgc attcctaagt gcatgcttgt agatgaccag
    11581 gcgctccgag aggcctttgg cccgggctgt ccggatataa tcggagccga tgacctccag
    11641 catgctggct cggctcaacc ggctgtactg ggctatgtag atgatcgcca aggtgaaagc
    11701 gggcagcacc aagtggtgaa ggacatcttt ggccaaatcc cagaaactgc ccgtaaggcc
    11761 cacggtgatc atgccgtggc cgggaaagag ggggaggtaa agggaaaagg ccaggatgag
    11821 catgatcccc agccagaaga cgggcatggc gaaaccgaag agggaaagaa cggtgatgaa
    11881 agagcttccc agggaagttg ggcgctgggc tacgatcacc ccggtcaaga cgccaagggt
    11941 gaccgccagg aagatggccg tgaggatgag aaggatggtg gccggaatct tttccaggat
    12001 caaatccatg acgggtttcc cctgaaagta ggaaaatccc aggtccccca ggagaacttt
    12061 ttccccatag atgaagagct gctccatgag gctcttgtcc agcccgaatt gcctgcgcac
    12121 ctccttgagg atttcttcgg tggcgccccc ggcttccccc acaaaaacca tggccgggtc
    12181 tccgggggcc agatgtaaga gggtgaagtt caggatcacg acaccgacta cgaccggaat
    12241 cgcctgcaag attctcctca ggagatacca gccgaaggag ctagaaggca ctgaatccct
    12301 ttcccttcaa tcctttttca ggcttaaccc gttttccctt tgcgccagta aaccgtgtcg
    12361 taggggttca agactcccca gacgtccatg ggaatcccat cgaaatcctt gttataaaag
    12421 gtatagagcc cgccctcctg aatccagatg agcggaagtt catccattaa aatctcctgc
    12481 accttatgat agaggttttt acgcttggca aaattctgt
//
LOCUS       DASPRR010000463         6180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_763689, whole genome shotgun sequence.
ACCESSION   DASPRR010000463 DASPRR010000000
VERSION     DASPRR010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763689"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VEL68_17035"
     CDS             <1..750
                     /locus_tag="VEL68_17035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ37731.1"
                     /translation="PVMFQLMALVPEFSSADLSSIHHFLAGGAPCPKSLIEIYLKKGV
                     VFNQGYGLTETAPGVSALRSEEAERKLGSAGKPMFHLDLCVVDDRDQTLPPMEMGEVV
                     LRGPNVFKEYWNLPKETAEAKRGGWFHTGDMGYLDEEGYLWLVDRKKDMYISGGENVY
                     PAEVEDVIYKMPQVKEVGVIGMPDPKWGEVGMAVVVTKPGMSLTDQEVLTFCQGKLAK
                     YKIPKKVIFAEALPRTLTGKVLKKELRGKYL"
     gene            1042..2622
                     /locus_tag="VEL68_17040"
     CDS             1042..2622
                     /locus_tag="VEL68_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ37732.1"
                     /translation="MSTASSLEMNLLSRATIGDIPKRTAAKYPDRTAIIFQGKRISFR
                     ELNESCCRFAHAFEELGAKKGDRIAFLTHNCLQYIYAWLGMAKIGCAIVPLNFMLKPF
                     EIEFIINHAEPKYFFVEDGLAPPVSEVAGKLKSVEKFGVIPLTGGKRPEGWLNIDDFL
                     TSSRRMDEPESEIHGDEMTTLLYTSGTEALPKGVMTTHQNYYATMMSGAVDLNISKDD
                     ITLLTIPLYHVAGKYLLLESINIAAAVVLQYAPNPTEILQLTQNEKVTSWVYPPTLYQ
                     ILPAMPNFKEFNLSSLRKCVAFGALMPVALLQQWKQILPQAEWRNYYGQTESSPLGST
                     LQPENFERKISSIGTPHTGVEMKIFDENDHEVPTGQVGEIVLRGPCVMKGYYKNEQKT
                     AETLRNGWLHTGDLGRFDDEGFLYFIDRKKDMIKTGGENVSSQEVEGLLFKQGKIMQA
                     AVIGMPDTIWGEAVTAVVVPFPGVTLTAEDIIAYCKASMAGYKVPKKVIFVSSMPTTP
                     SGKVLKRLLKEQIVKQNP"
     gene            2787..3950
                     /locus_tag="VEL68_17045"
     CDS             2787..3950
                     /locus_tag="VEL68_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ37733.1"
                     /translation="MDFRFSDEQKAFKDQVLKFSQKELAPLAEEADWKAEFCWEAWKK
                     MGGFGLLGITFPEAYGGSGADVVTACLAGEAVAQGGAEGGLCLAWGAHTYLCGDTILR
                     HGTEEQKKKYVPDLASGEWVGAMGLTEPGAGSDAASVRTTAVRKGDYYYLNGTKMFIT
                     NGPIADVMVIIAVTDKEKKGAGISAFIVEKNYAGFSVGRELKKMGVRASTTGELVFDD
                     CPVPAENILRREGEGFLIALGTVEWDRSTLLAPSLGGLEYALEACVSYAKERRQFDRP
                     IASFQAIQHKLADMKVIIEAMRLLIYRVAWLKDQGKGINPVEAAVAKLYVGELGMKAA
                     HDAVQIHGGYGFMHEYPVERLYRDTRLGSIGGGTSEIMRLIIARSIFAAGKPI"
     gene            4100..5254
                     /locus_tag="VEL68_17050"
     CDS             4100..5254
                     /locus_tag="VEL68_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ37734.1"
                     /translation="MDYELSEEQKKFKADFRSFCETEIAPQAIKIEQEGAFSYENHHK
                     LAEIGFLGLPFPEPYGGGGKSLLACVLAWEEVARACPSTYLSSGIGCLLPGVAVNFFG
                     TGEQKEKYLRGLARGEKIAAWAVTEPQTGSDTAAIKTEARWGGTSYVLNGTKAFVTNG
                     PIADWAVLVAVTDAGAAPNQRMSAFLVEKGTPGFSAGQPLDKMGARGSPTSDLILQNC
                     NLPGSRVLGEKGKGYSLLEKVQQFGRLSSAAFSLGVSQACLEEAIRYAQKRQAFGRPI
                     ANFQEISFKVADMQMFVDTGRLLLHRTAWMMDEGMDVDSDLSIAKLFLSEAATWCAAN
                     AVQIHGGHGFLKGVKVEQLYRDSKLGELRDGTSEMQRRKIALNLLGEGFK"
     gene            5428..5976
                     /locus_tag="VEL68_17055"
     CDS             5428..5976
                     /locus_tag="VEL68_17055"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXZ37735.1"
                     /translation="MLLKGGLHIHTTCSDGRLTPQEVAGAYQEKGFDFIAFTDHDYLL
                     KPETDGIYEKVKSDMIIFSGVELTVFAKGYIHVSRVNGEKEVLHILNHLGDYNLSREQ
                     MMDRIRTLGGMFPLDTVEITSKGFRCQEWEILEIPYPKLAADDSHSREGIGRAWVELE
                     AERNKDSILRAIKRGDFWNCFR"
BASE COUNT         1506 a         1573 c         1829 g         1272 t
ORIGIN      
        1 ccggtcatgt tccagttaat ggccctggtc ccggagtttt catccgcgga cctctcctcc
       61 atccaccatt ttctcgctgg cggagcgccc tgccccaaat ccctgatcga aatctatctc
      121 aagaaaggag tagttttcaa ccaggggtac ggtttgacgg aaaccgcccc aggggtttca
      181 gcccttcgct cggaagaggc ggaacggaag ctggggtcgg caggaaaacc catgttccat
      241 ttagatctct gcgtcgtaga tgaccgcgac caaacgcttc cgcccatgga aatgggggag
      301 gtggtactga gggggcccaa tgtcttcaag gagtattgga acctgcccaa agagaccgca
      361 gaggccaaaa gaggagggtg gttccatacc ggagacatgg ggtacctgga tgaggagggc
      421 tacctctggc tggtggaccg caagaaagat atgtacatca gcgggggaga gaatgtctac
      481 ccggccgagg tggaagacgt gatttacaag atgccccaag tgaaggaagt gggggtcatt
      541 gggatgccgg atccaaaatg gggagaggtg gggatggctg tggtggtgac caaacccggg
      601 atgagtctaa cggaccagga ggttctgacc ttctgccagg ggaaactggc taagtataaa
      661 atccccaaga aagtgatctt tgccgaagcc cttccgcgaa cgctcaccgg caaagtgctg
      721 aagaaagaac tgaggggcaa gtacctctag aggagagcca tcatagggtg caggcccaaa
      781 agacattgat tctttgttat tgcctcaagg cccgcagggc tgatcctgga ggacgagcct
      841 ggttttcaag ctttgcctat ggcgggccga tcctcaagcg gggcggtcgt tttttcgcgg
      901 agttgacttt tcccccccct ttttgctaaa agagtgctga ataatttaag caatggagga
      961 gggtaagcca ggaagaaaga aatatcagcc ccggttcttt ccatctaaca gcagtagcga
     1021 ttcaccatgg agggtttcaa gatgtccacg gcatcctctc tggaaatgaa ccttctctcc
     1081 cgggcgacca tcggcgacat tcccaaacga acggccgcca aataccctga ccggactgcc
     1141 attatcttcc aaggcaaacg aatatccttc cgggaactga atgaaagctg ctgccgcttt
     1201 gcccatgcct tcgaggaact gggggcgaag aaaggagatc gaatcgcctt cctcacccac
     1261 aactgtctcc agtacatcta cgcctggctg ggaatggcca agatcggatg cgccattgtt
     1321 cccctgaatt tcatgctgaa gcccttcgag atcgagttca tcatcaacca tgcggaaccc
     1381 aaatacttct ttgtggaaga cggcctggcc cccccggtgt cggaggtagc tggaaagttg
     1441 aagagtgtcg agaaattcgg ggtcatccct ctcacgggtg ggaaaaggcc ggaggggtgg
     1501 ctgaacatcg acgatttttt gacttcctcg cgaaggatgg atgaacctga gagcgaaatc
     1561 catggcgatg agatgacgac ccttctatac accagcggaa cggaggccct tcccaaagga
     1621 gtcatgacca cccatcagaa ttactacgcg accatgatga gcggggccgt ggacctgaac
     1681 atcagtaaag atgacatcac tctcctgacc atccctctct accatgtcgc cgggaaatac
     1741 ctccttctgg aatcgatcaa tatcgcggcc gcggtggtgc tgcaatatgc ccccaacccc
     1801 accgagattc tccagttgac ccagaatgaa aaggtcacct cttgggtcta tccgccgact
     1861 ctgtaccaga ttctgcccgc catgccgaac ttcaaagaat tcaacctttc ttccttgcgc
     1921 aaatgcgtcg ccttcggagc tttgatgccg gtggccctgc tgcagcagtg gaagcagatc
     1981 cttccccagg cggaatggag aaattactac ggtcaaacgg agtcttcccc cctgggatcc
     2041 actttacagc cggagaattt tgaacggaag atcagcagca tcggcacacc ccacactggg
     2101 gtcgagatga aaatcttcga tgagaatgat catgaagttc caaccgggca agtgggggaa
     2161 atcgtcctgc ggggcccctg cgtgatgaag ggatactaca aaaacgagca gaagaccgcc
     2221 gagaccctgc gcaacggctg gctccatacg ggagacctgg gtcgcttcga cgacgagggc
     2281 tttctctatt tcatcgaccg caagaaggac atgatcaaga ccggcggaga gaatgtctcc
     2341 tcccaggaag tggagggact tctgttcaaa caggggaaga tcatgcaggc agcggtgatc
     2401 gggatgcccg acacgatctg gggagaggct gtgaccgctg tggtggttcc ttttccgggg
     2461 gtcactctga ccgcagaaga cattatcgcc tactgcaagg ccagcatggc cggatacaag
     2521 gtgcccaaaa aagtaatctt tgtctcctcc atgccgacga cccccagcgg gaaggtactc
     2581 aaacggctgc tcaaggagca gatcgtaaaa cagaaccctt gatgtttcga gttccgggtt
     2641 tccggttgct cctttctcgg tagttgccat cagccctgga accagtgacc cgcgacgagc
     2701 agccagggac aattttcaat ttctgactcc gcacgggtga ccgagcggtc gccgctaaga
     2761 gctttctatt agagaacgga ggaaagatgg atttccgctt ttcggatgaa cagaaggctt
     2821 ttaaggacca ggtgctgaag ttcagccaaa aagaactggc ccccctggcc gaggaggcgg
     2881 actggaaggc ggagttctgc tgggaggcct ggaagaagat ggggggcttc ggacttctgg
     2941 gcataacttt tccagaagcc tatgggggaa gcggggcaga tgtagtaacg gcctgcctgg
     3001 ccggggaggc ggtagcacag ggaggggcgg aaggggggct gtgcttggct tggggagccc
     3061 atacctacct ctgcggtgac acgattttgc gccatggcac cgaagagcag aagaaaaagt
     3121 atgtacccga cctggcttcc ggggaatggg tgggggccat gggtctcacc gagccgggag
     3181 ccgggtcgga tgccgcctcg gtccgcacca ccgccgtccg gaagggtgat tattactacc
     3241 tgaacgggac caagatgttc atcaccaatg gccccatagc ggacgtcatg gttataatcg
     3301 cggtcacgga caaggaaaag aaaggggccg ggatctcagc ctttattgtg gaaaaaaatt
     3361 atgccgggtt ttccgtcggc agggaactga aaaaaatggg agtaagggct tccactaccg
     3421 gcgagctggt ttttgacgac tgcccggtgc cggcggaaaa catcttgcgg agggaaggag
     3481 aggggttttt gatcgctctc ggaacagtgg agtgggaccg gtcaaccctc ttggctccct
     3541 cgctcggagg attggagtat gccctggaag cttgcgtgtc ctacgccaag gaacgccggc
     3601 aatttgaccg ccccattgcc tccttccagg ccatccagca caagctggcg gacatgaaag
     3661 taatcatcga agccatgcgc ctccttatct accgggtggc ctggctgaaa gaccagggaa
     3721 aagggataaa cccggtggaa gcggctgtgg ccaagcttta tgtaggggaa ttgggtatga
     3781 aggccgccca tgacgccgtg cagattcacg gcggttacgg attcatgcat gagtaccctg
     3841 tagagaggct ttaccgggat acccggctgg gttccatcgg cgggggaacg tcggagatca
     3901 tgcgtctcat catcgcgcga tccatctttg ccgcgggaaa accgatataa gccaggttgg
     3961 aggtaggaag tttgaggctg gaggcacgag aaagagactt gaaaggatac aggggcaggg
     4021 acggaaaaag aagcgtagag cgtaatagct tatggttaat agctgaaggc ttatagctcg
     4081 tagctgatgg gagttttgca tggattacga attgagcgag gagcaaaaaa aattcaaggc
     4141 cgactttcgt tccttctgcg agacagagat tgctccccag gccataaaaa tagaacaaga
     4201 aggggccttt tcttacgaaa accatcacaa attggcggag atcggattcc taggcttgcc
     4261 tttcccggag ccctacgggg gagggggaaa gtctctcttg gcctgcgttc tggcgtggga
     4321 ggaagtagcc cgggcctgtc catccactta cctgtccagc ggtatcggct gtcttttgcc
     4381 tggagtggct gttaactttt tcgggaccgg ggaacagaag gaaaaatacc tgcgcggatt
     4441 agcccgggga gagaaaatcg ctgcctgggc cgtgaccgaa ccccagaccg gctccgacac
     4501 ggcggccata aagaccgaag cccgctgggg gggaacttct tacgtgctca acgggacgaa
     4561 ggcatttgtg accaatggtc ccatcgccga ctgggcggtt ctggtggcag tcaccgatgc
     4621 gggcgctgct cccaaccaaa ggatgagtgc cttcctggta gaaaagggaa cccccggttt
     4681 ttctgccggg caacctctgg ataaaatggg cgcgagagga tcgcccacct ccgacctgat
     4741 cctgcaaaac tgtaacctcc cagggagccg ggtgctcggc gaaaaaggga aaggatactc
     4801 cctgctggaa aaagtccagc aattcggccg cctttcttcg gcggccttca gcctgggggt
     4861 aagccaggcc tgcctggaag aggcgatccg ctatgcccag aaaaggcagg ctttcggaag
     4921 acccatcgcc aactttcagg agataagctt caaagttgcg gacatgcaga tgtttgtgga
     4981 caccggccgc ctcctcctgc accggactgc gtggatgatg gatgagggga tggatgtgga
     5041 ctcggacctt tccatcgcca agctcttctt atccgaagcc gccacttggt gcgcggccaa
     5101 cgccgtccag attcatggcg gccatggatt cctaaaaggg gtcaaagtcg aacagcttta
     5161 ccgggactcc aaactgggag aactccgtga cgggacctcg gagatgcagc gccgaaagat
     5221 cgccctcaat cttctggggg aaggctttaa atgattgggc aattgcatat ttcgcattgc
     5281 gaattaaaac taagatattt tgcaataatg aaattcgcaa ttgccaattc gcaattcgct
     5341 attgaaaatc aaacccaggc atcctgcccc gtcatggcaa tgccgaggta aatataagat
     5401 gcccatgtcg gaggccgagt tttaaggatg ctgctcaaag gtggccttca tatccatacg
     5461 acctgctcgg acggcaggct gactcctcag gaagtcgctg gcgcatacca ggaaaagggg
     5521 tttgatttta tcgctttcac ggaccatgac tatctgttga aaccggagac cgatggaatt
     5581 tatgaaaagg tgaagtcgga catgatcatc ttctccggcg ttgaattgac ggtctttgca
     5641 aaaggataca tccatgtgag ccgggtaaac ggggagaagg aggtcctcca cattctgaat
     5701 catctgggag actacaacct gtcccgcgag cagatgatgg accggatccg gaccctgggc
     5761 ggcatgtttc ctctggacac ggtggagatc acctcgaagg ggttccgctg ccaggagtgg
     5821 gaaattctcg agatccccta ccccaagctt gccgccgatg actcccacag tcgggaagga
     5881 atcggccggg cctgggttga gctggaagcc gaacgaaaca aggactcgat tctgagagca
     5941 ataaagaggg gagatttctg gaactgcttt cgataatgcg gaatagaatt aaagacaaaa
     6001 atggagtttc ttaattccgc atctcgtact ccgcattcca aatttgaaag gggacgttat
     6061 gtcgagaagg gttgcgatct gcgcggtggc ccagatcaag aacgagccgg acatctggta
     6121 caagcgtttt cagggcatgg tcctggattg tgtagagcag attctgaagc agaccggagt
//
LOCUS       DASPRR010000464         3204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_597496, whole genome shotgun sequence.
ACCESSION   DASPRR010000464 DASPRR010000000
VERSION     DASPRR010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597496"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VEL68_17060"
     CDS             <1..533
                     /locus_tag="VEL68_17060"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXZ37736.1"
                     /translation="RILAELEKCLSGQGTEPFEPTPQGLWEWMAGEIPLLAGVKPFNS
                     PEGFRLLGERKGGDFSPFRVGAPASSFAEDGLNLFCVEWTFGTEELSSYSTFTQQAEK
                     SPCLFLHAGDAARLGLGDKDRIRIELERGYLEVEVSTAENMAPGVMILPRHRALSWQV
                     MPELPARVTADKIKKI"
     gene            633..1628
                     /gene="nuoH"
                     /locus_tag="VEL68_17065"
     CDS             633..1628
                     /gene="nuoH"
                     /locus_tag="VEL68_17065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXZ37737.1"
                     /translation="MGTWIAGLILLIIKLVVVLGVLLGIAMYLVLAERKLLARLQVRL
                     GPNRAGKFGILQPLADVIKLLTKEDTVPAEADRFIFLFAPAIIALTTLLLFAVVPFGE
                     SLTFRDRPIPLVVADLDVGLLFLFALFSLGVYSVALGGWSSNSKYSLIGGLRGAAQMI
                     SYELPLVLSLVPIIMLAGSFSLVDMVKAQARYPFILVQPVSFLLFFFSAAAEIKRIPF
                     DLPEAENELVAGYHTEYSGMRFGLFFLGEYISLIVLGSLVTVFFLGGWRGPFFPPLAW
                     FALKVLAVVFVMIWMRATLPRLRYDQLMKLGWKVLIPVALLNIVLTGMVMLAIKS"
     gene            1720..2253
                     /gene="nuoI"
                     /locus_tag="VEL68_17070"
     CDS             1720..2253
                     /gene="nuoI"
                     /locus_tag="VEL68_17070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HXZ37738.1"
                     /translation="MSELLEGLRGLIEGFGTVLHHLFRRPVTEQYPEYKRKLPERSRA
                     RIILTRDPDGGERCVACYLCSAVCPVSCISMESAEREGGRRYARWFRINFSRCIFCGF
                     CEEACPTLAIQLTPDFEISRDEILKLVYEKEDLLVDHGGKNREYNFYRHAGVAVVGDK
                     GTHINEEAPVDIKSNLP"
     gene            2348..2878
                     /locus_tag="VEL68_17075"
     CDS             2348..2878
                     /locus_tag="VEL68_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXZ37739.1"
                     /translation="MNLYAAIFYLLSVILLASTVVAVTRRDAIHAVVYLIISFIATGL
                     IFYLLGAPFLALLEIIVYAGAIMVLFIFIVMMLRLTPSRRTWKAALSQWAPAIVLGGL
                     SLILAAVTIPADPASSPALKTLTAGPRELGRFLFQKYWFPVEIASFLLLIALVGALFL
                     GRQEKKAGKTTRQEGS"
     gene            2875..3180
                     /gene="nuoK"
                     /locus_tag="VEL68_17080"
     CDS             2875..3180
                     /gene="nuoK"
                     /locus_tag="VEL68_17080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXZ37740.1"
                     /translation="MIVPFSHVLFLAAILFLLGAACAVARRNLIMILIGVEIMLNAAG
                     LVFVGASLRWEHLEGQAFVIFILTLAAAEVAVGLALIVTSYRRTGSVNADRYNLLKG"
BASE COUNT          581 a          902 c          918 g          803 t
ORIGIN      
        1 ggaggatttt ggccgagctg gaaaagtgcc tgtccggcca aggaaccgag ccattcgaac
       61 caactccgca aggtctatgg gaatggatgg ccggggaaat tccccttttg gccggggtca
      121 aacccttcaa ctctccggag ggattccgtc tccttgggga aagaaagggc ggggattttt
      181 cccctttccg ggttggcgcg cccgcatcct cttttgcgga ggatggtctt aacctcttct
      241 gcgtcgaatg gacctttgga acggaggagc tttccagtta ttcgaccttt acccagcagg
      301 cggaaaagag cccctgcctg tttctccatg cgggggacgc cgcccggctg gggttgggtg
      361 ataaggaccg gattcggatc gaattggaga gggggtattt ggaagtggaa gtctccaccg
      421 cggagaatat ggccccagga gtgatgatat tgccgcggca ccgggcgctt tcctggcagg
      481 tgatgcccga gttgccagcc agagtgacag cggacaagat taagaaaatc tagtcacttg
      541 ttgctggcca cggatttttt gctgtcccct aaaagaaggg gaagcgcccc gcaacgagca
      601 tcgagcaaca agcaaagagc gacgaattct aaatgggaac ctggatcgct ggactcatcc
      661 ttctgatcat caagttggtt gtggtgctgg gtgtcctttt ggggatcgcc atgtacctgg
      721 tccttgccga acggaaactc ctggcccggc tccaggtccg cctcggcccg aaccgagcgg
      781 gaaagttcgg aatcttgcag cccctggcgg atgtgatcaa gctccttact aaggaagata
      841 cggtgccggc agaagccgac cgattcattt ttctgttcgc cccggcgatc atcgctctaa
      901 ccacccttct cctgtttgct gtggttccct tcggagagag cctcactttc cgggaccggc
      961 cgattcccct ggtggttgct gacctggatg taggtcttct tttccttttc gcgctgttct
     1021 ctctcggggt ttacagcgtg gccctgggag ggtggtcctc caactcgaag tacagtttga
     1081 tcggaggact gcggggagcc gcccagatga tcagctatga acttcccctg gtcctttccc
     1141 tggtaccgat tatcatgctc gccggctcat tcagcttggt ggatatggtg aaggctcagg
     1201 cccgctatcc tttcatcctg gtgcagccgg tttctttcct tctctttttc ttcagtgccg
     1261 cggcggagat taaacgcatt cccttcgatc tgcctgaggc cgaaaatgaa ctggtggccg
     1321 gttaccacac ggagtacagc ggcatgcgct tcggtctctt ctttctcggg gaatatatca
     1381 gcctgatcgt tttgggctcc ctggtaaccg ttttcttttt gggcgggtgg cgagggcctt
     1441 tcttcccgcc cctggcctgg ttcgccctga aagtcctggc ggtggttttc gtcatgatct
     1501 ggatgcgggc gactctcccc cggctgcgct acgaccagct gatgaaactg ggctggaagg
     1561 tactaatccc tgtggccctg ctgaacatcg tccttaccgg gatggtgatg ttggcgatta
     1621 aaagctgata gtttcgagtt tcaagtttcg agtttcgggt taacctccta cctagaactc
     1681 gaaacccgaa actcgaaact gtaagaggtc aaagaataga tgtctgaact tcttgagggt
     1741 ctaagagggt taattgaggg cttcgggacc gtcctccatc accttttccg gcgcccggtc
     1801 accgagcagt acccggaata caagcggaaa cttccggagc gttcccgcgc caggatcatc
     1861 ctcacccggg acccggacgg gggcgagcgc tgcgttgcct gttacctatg ttccgccgtg
     1921 tgcccggtca gctgcatttc catggagtcc gccgaacggg aaggtgggcg gaggtacgcc
     1981 cgctggtttc gcatcaattt ttcccggtgc atattctgcg ggttctgcga ggaggcttgc
     2041 ccgaccctgg ccattcagct caccccggat tttgagatct ccagggatga gatcctgaag
     2101 ctggtgtatg agaaagagga cctcctggtc gatcacgggg gaaagaaccg ggaatacaac
     2161 ttttaccgcc acgcaggcgt ggccgtcgtc ggggacaaag gaacccatat caacgaagag
     2221 gcgccggtgg atataaagag taacttgccg taagaagttt cgggtttcga gttccaggtc
     2281 ttagcctatt ttttttatcg agtatttaac ttgaaacccg aaactcgaaa ccctttggag
     2341 attctccatg aatctatacg ccgccatatt ctatcttctt tcagtgatcc tcctggcatc
     2401 tacggtggtg gccgttaccc ggcgggatgc catccatgcg gtggtttacc tcatcatttc
     2461 ctttatcgcc accggcctga tcttttattt gctgggggcc ccgttcctcg cccttctgga
     2521 gatcatcgtc tatgccgggg ccatcatggt ccttttcatc tttatcgtga tgatgctgag
     2581 gttaacccct tcccggagga cctggaaggc cgccctgtcc cagtgggctc cggccatcgt
     2641 tctgggcgga ctctcgttga ttctggccgc ggtcacgatc ccagccgacc cggccagcag
     2701 cccggccttg aagaccctca cggccggacc ccgagagctc ggccggttcc ttttccagaa
     2761 gtactggttc ccggtggaga tcgcttcttt cctcctgctc attgccctgg tgggagctct
     2821 cttccttggt cggcaggaga agaaggccgg aaaaactacc cgccaggagg gatcatgatc
     2881 gtccccttca gccatgttct ttttctggcc gcgattcttt tccttctggg ggcggcctgc
     2941 gccgtcgccc ggcgcaacct tatcatgatc ctcatcgggg tggagattat gctgaacgcc
     3001 gctggcctgg ttttcgtagg ggcctccctc cgttgggaac acctggaggg ccaggctttc
     3061 gtcatcttca tcctgaccct cgccgccgcc gaggtggcag tgggtctggc gctgattgtt
     3121 acctcttacc gtcgtaccgg gtcggtgaac gcggaccggt acaacctcct gaagggatag
     3181 atgcaggcct atcttttctt aact
//
LOCUS       DASPRR010000465         7381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1885494, whole genome shotgun sequence.
ACCESSION   DASPRR010000465 DASPRR010000000
VERSION     DASPRR010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885494"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1045
                     /locus_tag="VEL68_17085"
     CDS             395..1045
                     /locus_tag="VEL68_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37741.1"
                     /translation="MKSNSWFLVILRSFEPIKTFRVERRLFLFLGIGGVLLLGLLGLG
                     IYQYYSLHRENARLRDETQQLRLQVSNLHQTLSRVAAENFPPLKIDELQVFWPPKAAG
                     FAVRFKLSQANPQGAPYFGTLVMVAKNEKLRPPAYRVIPEMPLEKGVPREPEKGKPFE
                     VKGQRFVEALFDNNPEDAFHALTVFIFSREGKLILQKTAEIPKREPESREGREMEE"
     gene            1042..1464
                     /locus_tag="VEL68_17090"
     CDS             1042..1464
                     /locus_tag="VEL68_17090"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXZ37742.1"
                     /translation="MKPKKKKLLGRFFSPQEQPLTILCEGISVVGSIQFGEGVVRLDG
                     RLEGKISGQGTLIIGEKGFLQGELEVNTLILNGRVEGQVSTTAITHITPTGRLFGKVQ
                     SSSLVIDPGGVLDGEGKTFRSGGIVPSPAQPSNPTSGH"
     gene            complement(1540..3108)
                     /locus_tag="VEL68_17095"
     CDS             complement(1540..3108)
                     /locus_tag="VEL68_17095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ37743.1"
                     /translation="MKILLIQPPYPFSEFPKPSSALMSLGTILRQEGKEVEVLDLLST
                     RYSTDKIDRCLARFQPDVIGVTSVTMNFPLAIQTLQYCKEKAPGAVAVMGGPHVTFTD
                     EDTLRKYPGVDVIVRGEGEETTRELIQALEKGQSLEKVRGLTFRQNGKVRKTENRPLI
                     PDLDVLPMPDRTLFPISRYLAMRVPVSVLSSRGCPMDCSFCVGHRMTGRRGRFRDPYQ
                     VVDEIEAAHRLGFEEVCIDDDLFTRKRSHVFTICDEIIRRGLKMDMYIFARVDTVDSE
                     LLRKLRAAGCKMICFGLESGNQKILDLADKRATPEKARRAVELCKEVGISPLGSFILG
                     LPGETRDTMEETISFAQSLGIPYGFHLLSPFPGTKIREKAAEYGLKILTDDWSLYDAD
                     HAVTETEGVSAREVQNFAQSFFGKLSANIEEMKRGTIAGTYQGPFREDMEKRVEVDFA
                     WKLLSGDLIEDKGMIPRSEVHAESGPLGPLAKRLAGSLSLPLSFVEIKLRKLADRKCL
                     LCREEDGMFRWEWR"
     gene            complement(3119..4555)
                     /locus_tag="VEL68_17100"
     CDS             complement(3119..4555)
                     /locus_tag="VEL68_17100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ37744.1"
                     /translation="MKRRISIFFLILFVYLAFIPGGARAQDDDLLRHPRDMKFPPLSF
                     QPPKADRTILSNGMVVYLLEDPELPLIRVSALIRTGSIQDPPDKSGLARVTAAVMRNG
                     GAEGRPFKEINDTLETLAAEIEFSMEMESGSGQLFARKQDFPKVLSIFADLLMWPAFD
                     SAQLDLAKKAEIEAIRRQNDNPEEIAYREFRRALYRGNPRGRIPTAESIESIRQEDLI
                     VFHRKYYHPTTLYLGISGDFKKEEMIPLLEKTFGSWNRSLIEIPSIDFPSPQEEKSIY
                     YAPKEIPSSTILLGHLSLPQIHPDYFPFKVLNFILGGGGFNSRLTQEVRSNRGLAYNV
                     GSFYSGRVGYGVFGALCQTQAKTTHQAISLLYEIIGGMKQSPPKAEEIEWAQATLINQ
                     FIFSFSSSAAVVGQQMRLEYDGLPDDYLKKYQERVAAVTAADLQRAAREYLHPEKSVL
                     VVVGKEEGFEKPLSSFGSVNRIELNPAP"
     gene            complement(4576..6084)
                     /locus_tag="VEL68_17105"
     CDS             complement(4576..6084)
                     /locus_tag="VEL68_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ37745.1"
                     /translation="MNLLYRSKSYLAWVLILFFLLPPAAFSFSLEGRVKEFTLENGMK
                     VLMLERHQSPTISFHIRFRVGAVDENVGATGTAHLLEHMLFKGTQTLGTRNYAEEEKV
                     LSRLDSVADSLEAEKAKGDKADKDFLKYLEESFRSLQAEHKKWVVKDEIERVYSQNGA
                     VGFNAFTSQDTTAYMVSLPSNRLELWARVESDRLLAPVLREFYSERDVVMEERRRSYD
                     SKPQRKMVENFLAASFMAHPYGRPIIGWASDIQSLNKRATEDFFRIFYSPANTTLAIV
                     GDVNPEAAMPVIKKYFERIPRQVLPPPLKTREPEQAGERRIQVETDANPELIMGFHKP
                     NSPHPDDAGCEIIEGILSYGRTSRLYRRLVEEEKVAVSASASNGFPGERYPNLFILSG
                     TPRHPHPLAELEKGLLEELERLKKEPVPDLELQKIKNQIQTSFLRKLDSNSSLAYWLS
                     YGQSLFGTWRYVTDRIQAYEKVTPEDVRRVAQKYFIPRNRTVAALVKPSETP"
     gene            complement(6250..6906)
                     /locus_tag="VEL68_17110"
     CDS             complement(6250..6906)
                     /locus_tag="VEL68_17110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXZ37746.1"
                     /translation="MLRLPKLLWFFFLLAFLACAPKKVHYPPYEPGKEIRYRETGIAS
                     WYGEDFHGRKTANGEVYDMNAMTAAHRTLPFNIRVRVTNLDNGQKTEVRVNDRGPFVP
                     GRIIDLSRSGAKELGMLGPGTARVLVEATGFSPGAAQSMEGTYAIQVGAFLEKENAYR
                     FREELARRHPKVRVVLWETNTKRFYRVRLGAFRTEEEAKRYFDQLRRENLSGFVVRED
                     "
BASE COUNT         1616 a         2030 c         2110 g         1625 t
ORIGIN      
        1 gtctggacac taataccatc accgtcagca aggaaaccct tcccaaggaa acggaaatcc
       61 tgatccttaa ggtttcaagg gaaggagacc tttagaatgg ggaatgcgga ttagaagcgt
      121 aggggcgacc cagtgggtcg cccctaaggt ttgttaccat cttgtttcca tcttgcgcac
      181 cttttgactt ttgagagaat aagatcaatt ctacccatcc tcttaccagc actgctaacg
      241 agagaaaaaa aggttttgtg aacaaaggga agaaatctga actttctttt gagctcccat
      301 tcgaattccc ttaaaacttg acaaatccgc ctaattcccc taatttttaa tgaggcggga
      361 tgatccttat tgatagccat taccaaccta gggtatgaaa tccaattcct ggtttttagt
      421 catcctccgt tccttcgaac cgatcaagac tttccgggtg gagaggcggt tgtttctctt
      481 cctgggaatc ggcggggtct tattgctcgg gcttctggga ttggggattt atcagtatta
      541 ttctcttcac cgggagaacg cccgcctgag ggatgaaacc cagcagttgc gcctgcaggt
      601 ttctaatctg caccagacct tgagccgggt ggcggcggaa aattttccac ccttgaagat
      661 tgatgaactc caggtatttt ggccgcccaa agccgcgggt tttgcggtcc gattcaagct
      721 gagtcaggcc aatcctcagg gggctcccta ctttggtacc ctggtcatgg tggccaagaa
      781 tgaaaaactc cgcccccccg cctaccgggt tatcccggag atgcccttgg agaaaggggt
      841 tccccgggag ccggaaaaag gaaaaccttt cgaagtaaag gggcagagat ttgtggaagc
      901 gctctttgac aacaacccgg aagacgcctt ccatgctcta acggtcttta tcttttcccg
      961 ggaaggaaaa ctcattttgc agaagaccgc cgagattccc aagcgtgagc cggagtcgag
     1021 ggaagggaga gaaatggagg aatgaaaccg aagaaaaaaa aactgctcgg ccgcttcttc
     1081 tccccccagg agcagccgct taccatactc tgtgaaggaa tctcggtcgt gggaagcata
     1141 caattcgggg aaggggtggt tcgcctggat ggaagactgg agggcaaaat atccgggcag
     1201 gggaccttga tcatcggaga aaaaggcttt cttcaaggtg agctggaagt taacaccctg
     1261 attctcaacg gccgggtgga aggccaggta tcgaccacgg caatcaccca tatcaccccc
     1321 accgggagac tcttcgggaa agtacaatct tcgagcttag tcatcgaccc ggggggggtc
     1381 ctcgatgggg aggggaagac cttcagaagc ggaggaatcg tcccttctcc cgcccaacct
     1441 tcaaatccga cctccggcca ttgattcctt tggccgaatc gccgtatggc aaaggcgcct
     1501 gccttacccc ggcccaagac cggagagaaa ttattcggcc tacctccact cccaccgaaa
     1561 catcccatcc tcttcccggc agagaaggca tttccggtcg gccagcttcc ggagcttgat
     1621 ttccacgaag gaaaggggga gagagagact accggcaagg cgcttggcaa gagggccgag
     1681 agggccgctt tcggcatgaa cttcactgcg gggaatcatg cccttatctt cgatcaagtc
     1741 tccagacagc agtttccagg caaagtccac ttccacccgc ttctccatat cctcccggaa
     1801 aggcccctgg taggtgccgg cgatcgttcc ccgcttcatc tcttcaatat tggcactcag
     1861 cttgccgaag aaagattggg cgaaattttg cacttctctc gccgagaccc cctcggtctc
     1921 ggttaccgcg tggtcggcat cataaagaga ccagtcgtcg gtaagaatct tgagcccata
     1981 ctctgcggcc ttttcccgaa tcttagtccc ggggaaaggg gacaagagat ggaacccgta
     2041 agggattccc aggctctggg cgaaagagat ggtctcctcc atggtgtcgc gtgtttctcc
     2101 cggcagcccc agaatgaagg agcccaaggg ggatatgccc acctccttgc aaagctccac
     2161 ggcccgccgg gccttttccg gagtggcccg cttgtcagcc aggtccagga ttttctgatt
     2221 gccgctctcc aggccgaaac agatcatctt gcagccggcc gctcttaatt ttcggagaag
     2281 ctctgagtct accgtatcca cccgggcgaa gatgtacata tccatcttca ggccacggcg
     2341 aatgatttca tcacagatgg taaagacgtg actcctcttg cgggtgaaga ggtcatcatc
     2401 gatgcagacc tcttcgaagc cgaggcggtg agcggcttcg atttcatcga ccacttggta
     2461 ggggtcccga aagcgccctc tccttccagt catgcgatgc cccacgcaaa aggagcaatc
     2521 catggggcag ccccggctgg agaggacgct gaccggtacg cgcatggcca agtagcgcga
     2581 gatggggaaa agggtccggt cgggcatggg aagaacatca aggtcgggga tcaatggccg
     2641 gttttcggtc tttcggactt tcccattctg ccggaaggtc agacccctca ctttttccaa
     2701 actctgcccc ttttcgaggg cctgaatcag ttcccgcgtc gtctcttcgc cctccccacg
     2761 gacgataacg tccaccccgg gatatttccg gagcgtgtct tcatcggtga aggtcacgtg
     2821 ggggcccccc atgacggcca cggcaccggg cgctttctcc ttgcagtact ggagggtctg
     2881 aatggccagg ggaaagttca tggtcacgct ggtgaccccg atcacatcgg gctggaagcg
     2941 ggccaaacac cggtcaatct tgtccgtaga ataacgggtg gagagcaggt ccagaacttc
     3001 aacctccttc ccctcctgtc tcaagatcgt gcccagggac atgagcgccg aggaaggctt
     3061 ggggaattcg gagaacggat agggaggctg gatcagaagg attttcatgt ttctcccttt
     3121 atggagccgg gtttaattca atgcggttga cggaaccgaa ggaggaaagg ggtttttcga
     3181 atccctcttc ctttcccacg accaccagga cggatttttc cggatgaagg tactcccggg
     3241 cggcccgctg caggtcagcc gcggtcacgg cggccacccg ttcctgatac tttttgagat
     3301 agtcgtcggg gaggccgtcg tattcgagcc tcatctgctg ccccacaacg gcggccgagg
     3361 aggaaaaaga aaaaatgaac tggttgatca gggtggcttg ggcccactcg atttcttccg
     3421 ccttgggcgg gctctgtttc atgcctccaa tgatttcata taggagggat atcgcctggt
     3481 gggtggtttt ggcctgggtc tgacagaggg ccccgaaaac cccgtatccc acgcggccgc
     3541 tgtaaaagct tcccacattg taggccagtc cccggttgga ccggacctcc tgggtcagcc
     3601 gggagttgaa cccgcccccc ccgaggatga agtttaaaac cttgaagggg aaataatccg
     3661 gatgaatctg gggcagggac aggtgtccca ggaggatggt cgacgaaggt atctccttgg
     3721 gagcgtagta aattgacttc tcctcttggg gggaaggaaa atcgatggat ggaatttcga
     3781 tgagcgatcg gttccagctt ccgaaggttt tttcgagaag ggggatcatt tcctccttct
     3841 tgaaatctcc ggagattccc aggtagaggg tcgtgggatg ataatacttg cggtgaaaga
     3901 cgatcaggtc ttcctgtcgg atgctctcga tggattctgc tgtgggtatc cggccgcgag
     3961 gattgccccg gtagagagcc cggcggaatt cccggtaggc aatctcctcc gggttgtcat
     4021 tctgcctgcg gatggcctcg atctccgcct ttttggctag gtcgagctga gcggaatcga
     4081 aggccggcca catgagcaaa tcggcgaaga tggacagaac cttgggaaaa tcctgtttcc
     4141 gggcaaagag ttggccgctt cccgattcca tttccatgga gaactcgatc tccgccgcca
     4201 aagtctccag ggtatcgttg atttccttga agggccggcc ctcggcccct ccgttgcgca
     4261 tgaccgcggc ggtcacccgg gccagcccac tcttatcggg ggggtcctga atcgagcccg
     4321 tgcggatcag ggcagagacc cggatcaggg ggagttccgg gtcttccagg aggtagacca
     4381 ccatgccgtt ggagagaatc gtgcggtcgg ctttgggtgg ctggaaagag agagggggaa
     4441 acttcatatc ccgggggtgt ctcaggagat catcgtcctg ggcgcgagcc cctccgggga
     4501 taaaagcaag atagacgaaa aggatgagaa aaaagatcga tatcctgcgc ttcatacttc
     4561 ctctgcccct ctgggtcaag gagtttcgga cggtttcacc agggcggcga cggttcgatt
     4621 tcgggggatg aagtattttt gggccacccg acgcacatct tccggggtca ctttctcgta
     4681 agcctgaatc cgatccgtga cgtagcgcca agtcccgaag agagactggc cgtaggagag
     4741 ccagtacgcc aggctggagt tggaatccag cttacggaga aaggaagtct gaatctggtt
     4801 ctttattttc tgcaactcca ggtccggaac cggctctttc ttcaggcgtt ccagttcctc
     4861 gagaagtccc ttttccagtt cggccaaggg gtggggatgg cgcggggttc cggagaggat
     4921 gaagaggttg ggataacgct ctcccggaaa gccgttggag gccgaagcgc ttaccgcgac
     4981 cttctcctct tccacgaggc ggcggtagag gcgggaggtc cggccgtagc tcagaatccc
     5041 ttcgatgatt tcacacccgg catcgtccgg gtgaggagag ttgggcttat gaaaccccat
     5101 gatcaactcg gggttagcgt cagtctccac ctggatgcgc cgctccccgg cctgttcggg
     5161 ctcccgggtt ttcagaggag gagggaggac ctgccggggg attctttcga aatacttctt
     5221 gatcacgggc attgccgctt ccggattcac gtcccccaca atggctaggg tcgtgttggc
     5281 cggagaatag aagatgcgga agaagtcctc cgtggcccgc ttgttcagac tctgaatgtc
     5341 cgaagcccat ccgatgatcg gccggccgta agggtgggcc atgaaggaag cggccaggaa
     5401 gttctccacc atctttcgct ggggcttgga atcgtaggaa cggcggcgct cctccatgac
     5461 cacgtcccgc tccgagtaaa attcccgaag aacaggggct agcagacggt ccgattcgac
     5521 ccgcgcccac agctcaagcc ggttggaagg gaggctgacc atataagctg tagtgtcctg
     5581 gctggtaaaa gcattgaaac ccacggctcc gttctgagag tagacccgct cgatttcatc
     5641 cttcacgacc catttcttat gctcggcctg gaggctccgg aaggactctt ccaagtattt
     5701 gagaaaatcc ttgtccgcct tgtctccctt ggctttttcg gcttcgaggg agtcggccac
     5761 agaatccagg cggctcaaga ctttttcttc ttcggcataa ttcctggtcc ccagcgtttg
     5821 ggtcccttta aagagcatat gctccagaag gtgagccgtg cccgtcgcgc ccacgttttc
     5881 atccaccgcg cccacccgga agcggatgtg gaaagaaatg gtcggggact ggtggcgctc
     5941 cagcatcaac accttcatcc cgttttccag ggtaaattcc ttgactcgcc cctccaggga
     6001 aaaagaaaac gccgccgggg gaaggaggaa aaaaagaatc aggacccagg ctaaataact
     6061 cttgcttcgg tacagaaggt tcactttgaa ctctccgaat tttttagaag caaaagaaat
     6121 ataacacaag aagcaggggg agaaaaggac ccacattaat tgcggattgg ggaatgcgga
     6181 ttggggattg aaaagagaaa acgagtctat tgaaattccg caatcggaag tccgcaatcc
     6241 tcaatcggat cagtcttccc gcactacaaa cccgctgagg ttttcccttc tcagctgatc
     6301 gaagtaccgc ttggcttcct cctcggtgcg gaaggcgccc aggcggacgc ggtaaaaacg
     6361 cttggtgttg gtctcccaaa ggaccacccg gactttggga tgcctccggg ccaactcctc
     6421 ccggaaccgg taggcattct ccttctccag gaaggcgccc acctggatgg cgtaggtccc
     6481 ttccatcgat tgggccgccc cgggggagaa acccgtggcc tccacgagga cccgggccgt
     6541 gcccggaccc agcattccca gctccttggc cccgctccgg gagaggtcga tgatccggcc
     6601 ggggacaaag ggcccgcggt cattgacccg aacctccgtt ttttgcccgt tatccaggtt
     6661 ggtgacccgc acgcggatat taaagggcag ggtccgatgg gcggcggtca tggcgttcat
     6721 gtcgtaaacc tcgccgttgg ccgtcttacg accgtgaaaa tcctctccgt accaggaagc
     6781 gatcccggtt tcccggtacc ggatttcctt cccgggttca taaggtgggt aatggacttt
     6841 tttcggggcg caggcgagga acgcgagaag aaaaaagaac catagaagct tcggaagtct
     6901 cagcatgggc ctctctgttt taaattcttc attctgcttt ccgctctgca ctgggcggag
     6961 tgcaaggcag ccctcaacct cacttaatac aaacgcaaaa ggtaatttgt ctttgcgaga
     7021 agcggagcga cgaagcatcc ccccaccctc tccctccccc tcgagagact gtgtcgtaat
     7081 tcgtcatgcc ggcagaagct cgtagagtcg agtctccgac cggcatccag gattcaaata
     7141 aattgaataa aaccgaacac tggattccgg ctttcgccgg aatgacgccc ttccccttag
     7201 ttgcgacaca gcctccgagg ggggagggtg gggagggggg gagattgccg cgcccttcgg
     7261 gctcctaatg acatgtgaca ttatttaatg cgtttgtact agttccgggc gctacggttc
     7321 agatttttcc cagttttttc tcctcccgtg tcgacagata gattcccagc agaaccaaag
     7381 c
//
LOCUS       DASPRR010000466         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_598048, whole genome shotgun sequence.
ACCESSION   DASPRR010000466 DASPRR010000000
VERSION     DASPRR010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598048"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VEL68_17115"
     CDS             complement(<1..816)
                     /locus_tag="VEL68_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37747.1"
                     /translation="MRKISSLGVILLLSLWAIFPFHDPEIRGKDPGRERIEDFIQRGV
                     EKGLNLDGPGAVAELMRAIEVDRENPLGYAFLAMAHLFLYETAFDEGEKKKCESSMLL
                     AVEDTQNRAEKRMGKDPKNAEACFSLAISRMVKNRWEIIRKNYFRAFREAQGVWDLLE
                     KTQELDPQNFDVYYPMGVLHYHLAQLSGIARWITSLFIASGDREKGLKEFDIAYQKGR
                     LMKDLAASSLLSAYSGYEKQPARALPLAKALKEKYPDNYNFSFALINILSDLGR"
     gene            complement(823..2070)
                     /locus_tag="VEL68_17120"
     CDS             complement(823..2070)
                     /locus_tag="VEL68_17120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ37748.1"
                     /translation="MTLLPQRYLILIILNAVYFFVYFHRISTGVLAPYLMEAFHASGA
                     SLGGMSSAYFYPYALSQPVVGILTDRFGARKVVTVCTLIEFLGALIFALAPTLLIAAL
                     GRGLIGLGAAGVFVPALKLFLPWFGTRSFGRMNVILLAVGNVGAIVATTPFAWFIQQA
                     GWRSSFFIISGILLLLAFLSRAYLQDNPPEYDPPSGAQMQSQSPKAGGFVDILKSRFF
                     WIMAALFFAYGGPLSTFQGLWGYPYLIDVFGYSRLEAGNLIMVIAFGVIAGGQVMGYL
                     TDKTFAGKKRQLLSACIALQALNWGLIAFVSPRFGSWVLGLVFFIMGMMLAGTLAAVW
                     AIVREESPPERLGTAMGLINPAPFLGVAALQPLTGYLMDRVGKTGGTFPVQAYQNAFT
                     LCLFSVLVAMVISFFLMKMKKAE"
     gene            complement(2212..3645)
                     /gene="gatB"
                     /locus_tag="VEL68_17125"
     CDS             complement(2212..3645)
                     /gene="gatB"
                     /locus_tag="VEL68_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXZ37749.1"
                     /translation="MEYEPVIGLEVHAQLLTRSKIFCGCSTLFGAEANTHTCPVCTGL
                     PGSLPVLNQKVVEFTVRAALATRCKIAPYSQFARKNYFYPDLPKGYQISQYELPLSTD
                     GYVEVQVDSQKKKIGLIRIHMEEDAGKLLHDLSSERGASSYVDFNRTGVPLIEIVSAP
                     DIRSPEEAAAYLRKLRAILMFLDICDGNMEEGSFRCDANISLRPKGQKAFGVKTELKN
                     MNSFRNVQKALDYEVRRQTALLDQGDRVIQETRLWDAARGVTASMRGKEEAHDYRYFP
                     DPDLVPLVIDPQWVEKIRAGLPELPDQKKGRFIRDYGIPEYDAEILTSSKALANYYEE
                     CLRTYNQPKIVSNWIMSELLRELKRDEREIEECPVPAGNLAEMLKMIEEGVISGKIAK
                     SVFEEMYRTGNRAQEIVKEKGLVQVSDTSTIEKAIDQVLAANPGEVEAYRKGKDKVFG
                     FFVGQVMKATQGKANPALVNELLKKKL"
     gene            complement(3658..>4431)
                     /locus_tag="VEL68_17130"
     CDS             complement(3658..>4431)
                     /locus_tag="VEL68_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXZ37750.1"
                     /translation="ISGHEPRDSTSVDLPVPDYTRVLGDDVRGMVLGVPQETFGKGID
                     PEVAGSVREAIEVLRKLGAKTVDVSLPHSDYALAVYYIIAPCEASSNLARYDGVKYGF
                     RAGGGEDLLDMYQETRWQGFGAEVKRRIMLGTYALSAGYYDAYYLKASRVRTLIRKDF
                     EEAFRRCDALILPTAPTPAFRLGEKVDDPLQMYLSDIFTIPCNLAGLPGLSLPCGFTG
                     EGLPIGLQILGNFFQEEKILRVAWAFEQNTEHHLRKPIL"
BASE COUNT         1001 a         1208 c         1281 g          941 t
ORIGIN      
        1 tcgtcccagg tccgaaagaa tattgatcaa ggcaaaggag aagttataat tgtccgggta
       61 tttttctttc agcgctttgg ccaggggcag agcgcgggca ggttgcttct cataaccgga
      121 gtaggcagac aacaggctgg atgcggctaa gtccttcatg agacggcctt tttgataggc
      181 aatgtcaaac tccttcaatc ctttctcccg gtcgccggag gcaatgaata aggaagtgat
      241 ccaccgggcg atcccgctga gctgggccag gtgatagtgg aggaccccca tggggtaata
      301 gacatcaaaa ttttgcgggt ccagctcctg ggttttttcc aacaagtccc agaccccttg
      361 ggcttcccgg aaggcgcgga aataattctt gcggatgatt tcccagcggt tcttgaccat
      421 ccgggatatg gccagggaga agcaagcctc ggcattttta gggtcttttc ccatccgctt
      481 ttcagcccgg ttctgggtat cctcgacggc cagcaacatg gaggattcgc acttcttctt
      541 ctccccctcg tcaaaagcgg tttcgtagag gaagagatga gccatggcca ggaacgcata
      601 gcccagggga ttttcccggt ccacctcgat ggccctcatc agctcggcaa cggctcccgg
      661 gccatcaagg ttgaggcctt tttccacacc gcgctggata aaatcctcga tccgttcccg
      721 gcccgggtct ttaccccgga tttccgggtc gtgaaaaggg aaaatcgccc aaagagacag
      781 aagaagaata acgcccaggg atgaaatttt tctcatggaa actcactccg ccttcttcat
      841 tttcataaga aaaaaggaga tcaccatggc gaccaaaacg gaaaacaggc acagggtgaa
      901 ggcattctgg taagcctgca ctggaaaggt cccaccggtt tttccgaccc gatccatgag
      961 gtacccggtg agtggctgga gggcggctac gcccaggaaa ggggcggggt tgataaggcc
     1021 catggcggta cccaatcttt ccggcggcga ctcctcccga acaatggccc agacggcggc
     1081 gagcgtcccc gccaacatca ttcccatgat aaagaaaacc agacccagaa cccatgatcc
     1141 aaatcggggg ctcacaaaag cgatcaatcc ccaattcagg gcttgtaggg cgatgcaggc
     1201 cgaaaggagt tgacgctttt tcccggcgaa ggtcttgtcc gtcaggtacc ccatcacctg
     1261 tccgccggcg atgaccccaa aagcgatgac catgatcagg ttgcccgctt ccagcctgct
     1321 gtagccgaag acatcgatca agtaagggta cccccataat ccctggaagg tgctgagggg
     1381 gccgccgtaa gcgaagaaga gggcggccat gatccagaag aagcggcttt tcaagatatc
     1441 tacaaacccg ccggcctttg gagattgcga ctgcatctgg gctccggaag gggggtcata
     1501 ttcagggggg ttatcctgga ggtacgcccg ggaaaggaag gccagaagaa ggaggatgcc
     1561 ggaaatgata aaaaaggaag accgccagcc cgcctgctgg atgaaccaag cgaagggagt
     1621 ggtcgccacg atggccccta cattgcccac agcgaggaga atcacgttca tccggccgaa
     1681 agaccgggta ccgaaccagg gaaggaagag tttcaacgcc ggaacgaaaa cccccgcggc
     1741 tcccagcccg atcaatcccc ttcccagagc ggcgatcagt agagtggggg ctagagcaaa
     1801 aatcagggcc cccagaaact cgataagggt gcagacggtt accacttttc gcgccccgaa
     1861 gcggtcggtg aggatcccca ccaccggctg gcttagggcg tagggataaa aataggctga
     1921 cgacattccg cccaggctgg cccccgaggc gtggaaggct tccatcaggt agggagccag
     1981 gactccggtt gaaatccggt ggaaatagac gaagaagtag acggcgttga ggatgatgag
     2041 tattagatag cgttggggga gaagggtcaa gggaagtgcc ttaagtgtgc ttaagtgtac
     2101 ttaagtgcct caagttagaa gtgctaaagt gatagagtct ggcgatttgt gtccgtgtct
     2161 ggagaaaaac cagaagaaac ttcatactaa cacgaaccac tgaccctgtt tttataactt
     2221 cttcttcagc agttcattga ccagggctgg gtttgccttg ccctgggttg ccttcatgac
     2281 ctgacccacg aaaaagccga aaaccttgtc cttccccttg cggtacgctt ctacttcccc
     2341 gggattggcc gccaggacct ggtcgatggc cttctcgatg gtcgatgtgt cgcttacttg
     2401 aaccagccct ttctctttga cgatctcttg agcccggttg ccggtccggt acatctcctc
     2461 gaagacggat ttggcgatct tcccgctgat caccccttct tcgatcatct tcaacatttc
     2521 ggcgagattc cccgccggga ccgggcattc ttcgatttct cgctcgtcgc gtttcagctc
     2581 ccggaggagc tcggacatga tccagttgct gacgatcttg ggctggttgt aggtgcgcag
     2641 gcattcctcg tagtaattcg ccagggcctt ggaggaagtg aggatttcgg cgtcgtactc
     2701 cgggattcca tagtcgcgaa tgaaacgtcc ctttttctgg tccgggagct cgggaaggcc
     2761 ggcccgaatt ttttccaccc actgggggtc gatgaccaga ggaaccagat cgggatcggg
     2821 gaagtaccga tagtcgtggg cctcttcctt ccctcgcatg gaagcggtca ccccccgtgc
     2881 ggcatcccag agacgggttt cctggatgac tcggtctccc tggtccaaaa gggcggtctg
     2941 tctccggacc tcataatcca atgccttctg gacattgcgg aaggaattca tgttcttcag
     3001 ttcggtcttc acaccgaagg ccttctgccc cttgggacgg agggaaatgt tggcgtcgca
     3061 ccggaaactt ccttcctcca tgttcccgtc gcagatgtcc aggaacatga ggatagcccg
     3121 aagcttcctc aggtaagcgg cagcctcctc cggggaccga atgtcgggag cgcttacgat
     3181 ttcgatgagg gggacgccgg tgcggttgaa atccacgtag ctggaggcgc cccgttccga
     3241 agaaaggtcg tggaggagtt tgccggcgtc ttcttccatg tgaatgcgga tgagcccgat
     3301 ctttttcttc tggctgtcca cctgaacctc cacgtacccg tccgtggaca ggggaagttc
     3361 atactgggaa atttggtagc ccttgggaag gtccgggtaa aaataatttt tcctggcgaa
     3421 ctggctgtag ggggcaatct tgcagcgcgt ggccagcgcg gcgcgcaccg taaattcgac
     3481 gaccttttga ttgaggaccg gaagacttcc ggggagcccg gtgcaaaccg ggcaggtatg
     3541 ggtattggcc tcggctccaa agagagtgga acaaccacag aaaatcttac ttcgggtcag
     3601 aagctgggcg tgaacttcca ggccgatgac cggttcatat tccatgaccg actccgttca
     3661 caagatgggt tttcgcagat ggtgttccgt gttctgttca aaagcccagg caacccgcag
     3721 aatcttttcc tcttggaaga aattgccgag aatctgaagg ccgatgggca gtccctcccc
     3781 ggtgaaaccg caaggaaggg acagaccggg aaggccggcc aggttgcagg gaatggtgaa
     3841 aatgtccgaa aggtacatct gcaaaggatc atccactttt tctccgagtc gaaaagccgg
     3901 cgtgggggcc gtgggaagga tcaaggcgtc gcacctccgg aaggcctctt cgaaatcctt
     3961 acggatcagg gttcgcacgc gggaggcttt gaggtaatag gcatcatagt agccggccga
     4021 aagggcatag gtgccgagca tgatccgccg cttgacctct gccccgaatc cctgccagcg
     4081 ggtctcttgg tacatatcca gaaggtcctc ccccccccct gcacgaaaac cgtatttcac
     4141 tccgtcgtag cgggccaggt tggagctggc ttcacaggga gcaatgatat aatacacggc
     4201 cagggcgtaa tcgctgtggg ggagagagac gtcaacggtc ttggcgccca attttctcaa
     4261 gacttcaatc gcttccctga ccgaaccggc cacttcgggg tcgattcctt tgccaaatgt
     4321 ctcctgggga actcccaaga ccattcctct tacatcatcc ccgagcactc gggtgtagtc
     4381 cggaaccgga agatcgacgg atgtggagtc ccggggttca tggccggaaa t
//
LOCUS       DASPRR010000467         5459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2364097, whole genome shotgun sequence.
ACCESSION   DASPRR010000467 DASPRR010000000
VERSION     DASPRR010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364097"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /locus_tag="VEL68_17135"
     CDS             <1..2284
                     /locus_tag="VEL68_17135"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXZ37751.1"
                     /translation="GTFTGAWLPGSRFEADGKARVSQAEFQWKRSMKPISILLNAAEA
                     DFAWRGESIQGNLNLNSADHGTLKGTFLIPLSARLSPSFIPEGPFKVSVQGQLRENGL
                     LSRLYPERVQKSRGEIGLDLNAGGTWSKPQWKGTVQISGAGFQITTPKGERKEEKNSS
                     GVFMEMPYAKAAVEWDSRGLVAVLVAMLNDNGRIEGTVASSDPPRTAFPRQGKIDFLW
                     AAFNLALLQPILPEGFHLEGKAEGKVKGSLLPDLHLDLSGGWKVSKGSLSWKGDKGQI
                     SAGINQADVDFLWRDERVQGNVSFALADFGSLKGNFRFPIPARPPFQADPAGSLQVTL
                     QGQAQEKGLLSAFFPGMVEETRGTIDLDFKAQGTWGQPDLQGSLQLTNAGASIPSLGI
                     RVEDLSSRWKLKNEQILVESLRVRSGPGSLEGAGTIWLKNWGMERFEGNLKGDKFQTL
                     YLPNMRIQSSPKLQFQGTPSRLSVRGEILLPEVNIYDVSAPGVAKTSSDLIMVDRPAE
                     PELPFSLDIQVRVVLGDRVEIRTETINAHLAGNMDLNIVGLKPEEMTARGEIRLTQGL
                     YGGYGLSLRIDRGRFTYSGGPVDNPALDILALRRSDDVEKLYNVKVGVAIFGTLKHPN
                     VKLYSQPVMKDEEILSYLILARPYDPQKGNLSLLVAGAGGLLSGDSLSVLDKLKSQLG
                     IDTVDIQSGSGSGGGDMTRSMVTVGKYLTPELYVSYGYSVFSEEYLLKLRYRLSKAWE
                     VETWRGNQTGINLYYRIDFF"
     gene            2486..2950
                     /locus_tag="VEL68_17140"
     CDS             2486..2950
                     /locus_tag="VEL68_17140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXZ37752.1"
                     /translation="MYNTILVPLDGSKLAECVLPHVETLVKGSQAKRVIFARVVEPFL
                     PPTSEYIFSDDQIKKVDKENRAFAEKYLKEIVGQAKYGSGVRIETTILSGNVPDSLAE
                     FANKNGADIIVIATHGRSGVSRWVWGGVADRILRSSCVPVLMVRAPGCVPGI"
     gene            3262..4788
                     /locus_tag="VEL68_17145"
     CDS             3262..4788
                     /locus_tag="VEL68_17145"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXZ37753.1"
                     /translation="MNVGSLLTMQANKFPDRTALIYENRRSTYREFNRRSNQFANALL
                     RFGLQKGDRVATLLFNSPELVEVFMGTAKTGGVFTPINFRLAAEEVIYLVNHSDARIF
                     VLGEDFLSLVEKILSQLPKVERFISVGKAPFPRAIEYEPLLQGSLEEEPGIAVSEEEE
                     CQMLYTSGTTGRPKGAVLTHGNILWNLVNTLLAREDKEGEIALVTGPLYHAAALNNHF
                     LIRLALAGASILMKHFDPRSFMEIIAREKVNVISGAPAMFHLLLNLPDVEKYDTRSIT
                     RCTLGSSTLPDDTKKKLLKLFPNAGGIYDVYGATEVSPTLSVLKAADSFRKTACVGPA
                     VPFLEVKVVDGQDQEVPRGKTGEIICRGPNVMKGYYKDPEGTAEALRGGWLHTGDIGR
                     MDEEGFIYIVDRKKDMIISGGENIYPREIEELLYHHPKIQEAAAVGMPDPLWGESVKV
                     FVALKRGMTMSQEEVIEYCKAHLAGYKKPKQVEFIDSLPRNPSGKVLKNLLRERQAES
                     "
     gene            complement(4919..>5459)
                     /locus_tag="VEL68_17150"
     CDS             complement(4919..>5459)
                     /locus_tag="VEL68_17150"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ37754.1"
                     /translation="SGRRPIPTGNASPFAYPVDIFPSADGYVSISAFNDKFWRNLCRA
                     LNLDSLAGDPRFNTPGKRLSNREELGSLLSKVLATRKTQEWLEILEEADVPCGPVHDY
                     DTIFSDPQVLHNQLVKTLPHSRLKEVRALGNPISFSLTPASEGRAAPLLGEDTDSILH
                     SLGFKPEEAKEFREKRIIL"
BASE COUNT         1271 a         1465 c         1541 g         1182 t
ORIGIN      
        1 aggaaccttc accggggcat ggcttcccgg ttcccgtttt gaagcagacg gaaaggcacg
       61 ggtctcccag gcggagtttc agtggaagag aagcatgaag cccatttcca tcctattgaa
      121 cgcagctgag gctgacttcg cgtggcgggg ggagtctatc caagggaacc tgaacttgaa
      181 ttccgccgat catgggactt tgaaagggac ctttcttata cccctttcgg cccgcctatc
      241 cccgtcgttc attccggaag gcccattcaa ggtttcggtc caggggcaac tccgggaaaa
      301 cgggcttctg tcccgtcttt atccggaacg ggttcagaaa agccggggag agatcggcct
      361 cgacctgaat gccgggggta cttggtcgaa acctcaatgg aagggcacgg tgcaaatttc
      421 cggagccggt tttcagatca ccacccccaa aggagaaagg aaagaggaaa agaattcttc
      481 cggggtcttt atggaaatgc cctacgccaa ggccgcggtc gaatgggatt cgcggggttt
      541 ggtggctgtc ttggtggcca tgctgaacga taacggaagg atcgagggaa ccgtcgcttc
      601 ttccgacccc cccagaacgg catttccccg gcaggggaaa atcgatttcc tctgggcggc
      661 cttcaatctc gccctcttgc agcccatcct gccggagggg ttccatctgg agggaaaggc
      721 cgaggggaaa gtgaaaggat ccttgttgcc cgatctccac ctcgacttgt ctggcggatg
      781 gaaagtgtcc aagggaagtc taagctggaa gggagacaag gggcagatca gcgcggggat
      841 caaccaggcc gatgtggatt tcctctggcg cgacgaaaga gtccagggaa atgtctcttt
      901 cgcgctggcc gatttcggct ccctcaaggg gaatttccgg tttcctatcc ccgcccgacc
      961 tcctttccag gccgacccgg cgggatccct gcaggtcacc cttcaagggc aggctcagga
     1021 aaaaggactc ctctcagctt tttttcccgg gatggtggaa gaaacccggg gaaccatcga
     1081 cctggacttc aaggcccagg gaacctgggg gcagccggac cttcaagggt ccctgcaact
     1141 gaccaacgcc ggggcctcca tcccgtctct ggggattcgg gtggaagatc tttcctcccg
     1201 gtggaaacta aagaatgagc agatcctggt cgaatcgctc cgggtccgct ccgggcctgg
     1261 atctctggaa ggcgcgggaa ccatctggct gaaaaactgg ggaatggagc gtttcgaagg
     1321 aaacctgaaa ggagataaat ttcaaaccct ctacctgccc aacatgagaa tccagagcag
     1381 cccgaagctc caattccagg ggactcctag ccgcctctcg gtgcgggggg agattctgct
     1441 gcccgaggtg aatatctacg acgtctctgc tcccggggta gctaaaacca gttccgacct
     1501 tatcatggtg gaccggccgg ccgaaccaga gctgcccttt tccctggata ttcaggtccg
     1561 ggtggtcctg ggcgatcggg tggaaataag aaccgagacg atcaatgccc acctggcggg
     1621 aaatatggat ttgaacattg tgggtttaaa gcccgaggag atgactgccc ggggggaaat
     1681 ccgtctaacc caaggccttt acggcggtta tggacttagc ctgcggatcg accgggggcg
     1741 tttcacctat tccggcgggc ccgtcgacaa ccccgccctc gacatcctgg ccttgcgccg
     1801 ctccgacgac gtggagaaac tgtacaacgt gaaagtgggg gtggctattt tcgggacttt
     1861 gaagcacccc aatgtgaagc tttattccca gccggtcatg aaggatgaag agattctctc
     1921 ctatctgatc ctggcccggc cttacgaccc gcagaaaggg aacctctccc tgctcgtggc
     1981 gggggctgga ggtctcctgt ccggagactc tctcagcgtg ctggacaagt tgaaaagtca
     2041 gctggggatc gacacggtag acatccaaag cgggagcgga agcggaggcg gggacatgac
     2101 gcggtcgatg gttaccgtgg gcaaatatct gaccccagag ctttacgtca gctacggcta
     2161 ttccgtgttc tccgaagaat accttttgaa actccgttac cggctctcca aggcctggga
     2221 ggtggaaacc tggcggggaa atcaaacggg gatcaatctg tactaccgga tcgatttttt
     2281 ttagaaataa cgagaggaca tccatccaac ccaggggcgg ttcgcgaacc gcccctagag
     2341 ccttatcatt tggtcatgaa tgcggaaaat acatagccct attcctctgc aagagcatct
     2401 aaatatctaa aaaggaagat attttggcgc ttgccttttg agctgcatcg aattttgaag
     2461 taattcttaa aaaagggagg gtgtcatgta taacacaatt ctcgttcccc tggatggatc
     2521 gaagctggcc gaatgcgttc ttccccatgt ggaaacgctg gtcaagggtt ctcaggccaa
     2581 aagggtgatt tttgcccggg tagtcgaacc cttcctcccg ccgacttctg aatatatttt
     2641 cagcgacgac cagatcaaga aggtggacaa agaaaatagg gccttcgctg aaaaatacct
     2701 caaggaaatc gtcggccagg ctaagtatgg gagtggggtt cggattgaaa ccacaattct
     2761 ctccggaaat gttcccgatt cccttgccga attcgccaac aagaatgggg ccgatatcat
     2821 cgtcatcgcc acccatggcc gctcgggagt cagcagatgg gtatggggcg gagtggctga
     2881 ccggatcctc cggtcttcgt gcgtgcccgt tctgatggtc cgcgccccgg gttgcgtgcc
     2941 gggaatctga actgatttcg gattgcggat tttcaaatcc gacactgtta atcgggatat
     3001 attcgtaggg gcacgatgtc cggccaaacg cggcatgccc ccgcgagctg ttgtatctta
     3061 cattctttat accccgctcg cttgaaatct gattccagcc ttgacaatcc tccccaattt
     3121 ttatagcttt ccaacagcga gtaggggcgg ttcgccaacc gggtaacggc tattccgatt
     3181 cataattttt atatctctga tctttctttg taatctgcgc aatctgcgga taaaaaacag
     3241 gtgcgaactc cggagggttt tatgaatgta ggttcactcc tgacgatgca ggcaaataaa
     3301 ttccccgatc ggacggccct catctacgag aataggaggt ccacttatcg ggaatttaat
     3361 cggcggagca accaatttgc caatgccctc ctgcgcttcg gcctgcagaa aggggaccgg
     3421 gtggcgacgt tgctctttaa ttcccctgag ctcgtcgagg tcttcatggg tacggcgaag
     3481 accggcgggg ttttcactcc catcaacttt cggctggctg ctgaagaagt aatctacctc
     3541 gtcaatcatt ccgacgcccg tatctttgtc ttgggagagg atttcctttc tctggtagaa
     3601 aaaatccttt cccagcttcc aaaggtggaa aggtttatct cggtcggaaa ggcacccttc
     3661 ccccgggcca tcgaatatga gcccctcttg caggggtctt tggaagaaga gcccggaatc
     3721 gccgtttcgg aagaagaaga gtgccagatg ctctacactt ccggaacaac gggaagaccc
     3781 aagggagccg ttctcaccca tgggaatatc ctctggaact tggtgaacac gctcctggca
     3841 agagaggaca aggaaggaga gattgccctg gttacaggtc ctctatacca cgctgccgcc
     3901 ctcaacaacc atttcctgat ccggttggcg ctggccggag ccagtattct gatgaaacac
     3961 tttgatcccc gcagcttcat ggaaatcatc gcgagagaga aagtcaatgt gatctccgga
     4021 gcgccggcca tgtttcacct cctgctcaac ctccccgacg tcgaaaaata cgacacccgc
     4081 tctattaccc ggtgcacttt ggggtcttcc accctgcccg acgataccaa gaagaagctc
     4141 ctcaagctct ttccaaatgc cggtggaatt tatgacgtct atggggccac ggaagtttcc
     4201 cccactctta gcgttctgaa agcagctgac tccttccgaa aaaccgcgtg tgtcggcccg
     4261 gcagttcctt tcctggaagt gaaagtcgtg gacggccagg accaggaggt tccacgcggc
     4321 aaaaccggtg aaatcatctg ccgcggccca aacgtcatga aaggatatta caaagacccc
     4381 gagggaaccg ccgaggccct caggggagga tggctccata cgggcgacat cggacgaatg
     4441 gatgaggagg ggttcatcta tatcgtggac cgcaagaagg acatgatcat cagcggcggg
     4501 gagaatatct acccccggga aattgaggaa ctcctctacc accaccccaa aatccaggag
     4561 gcggcggcag tgggaatgcc cgatcccctt tggggagaat cggtgaaagt cttcgtggct
     4621 ttaaagaggg ggatgaccat gtcccaagaa gaggtcatcg aatactgtaa agcccatctg
     4681 gccggctaca agaagcccaa acaggttgag ttcatcgact ccctcccccg aaatccctcg
     4741 gggaaggtcc ttaaaaacct tttaagagag cggcaggcag aatcttgaca tgaacgaacg
     4801 agagagcaac ggcacgcgtg aagcgaagcg ggcatgggat aaatccgacc gatcagacag
     4861 atcggacggg tccggtcgaa aatgccgccc agccggtacg ccttagccct tgcagcggct
     4921 ataaaatgat tcttttttcc cgaaactctt tcgcttcttc cggtttaaat cccaaagagt
     4981 gaagaatgga atcggtatct tccccaagta acggcgctgc tcgaccttca ctggccgggg
     5041 ttaggctgaa ggaaatgggg ttcccaagag cgcggacctc cttcaggcgg ctgtggggaa
     5101 gcgtcttgac caactggttg tgaaggacct ggggatcgga gaaaatcgta tcatagtcat
     5161 gaaccgggcc gcagggaacg tcggcttcct ccaggatttc aagccattct tgggttttcc
     5221 ttgtagccag cactttggag agaagggacc ccaattcttc tcggttagaa agtctttttc
     5281 ccggagtgtt aaagcgagga tcaccggcca gggaatccag gtttaacgcg cggcacagat
     5341 tccgccagaa cttgtcatta aaggcgctga tgctcacgta cccgtctgcg ctggggaaga
     5401 tatcgaccgg ataagcgaaa ggactggcat tccccgtggg gatggggcga cgtccgctg
//
LOCUS       DASPRR010000468         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_115978, whole genome shotgun sequence.
ACCESSION   DASPRR010000468 DASPRR010000000
VERSION     DASPRR010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115978"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VEL68_17155"
     CDS             complement(<1..185)
                     /locus_tag="VEL68_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HXZ37755.1"
                     /translation="MPNEGNPIRNPQSEIRNSDRGIIFNIQRFSIHDGPGIRTSVFFK
                     GCPLRCDWCSNPESQDPL"
     gene            complement(249..2645)
                     /locus_tag="VEL68_17160"
     CDS             complement(249..2645)
                     /locus_tag="VEL68_17160"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HXZ37756.1"
                     /translation="MKEKLEDIKSRLVIKAVRNVRRGYHEEEASRGMYRPGIKLDLQR
                     SRLLTESFKETDGQPMVLRRARGFAKVLNQMDLYIQDWEKIVGSNASTPQGLYFGIDM
                     NWRSVQRVVSGQEGQTLLDDAGRAELAGLIEYWRGKSMSDRQQELFTGDLLKYWQYEG
                     TFLWSHWSELGIPDYEKIFKVGVKGLIRQAQDRLQALDREVPLNYLEQKEFLQAAVIA
                     LEAVVNFAHRYARMAREMAARANQEEDRRRLEEIAAVCDWVPENPPRSLLEALQSFFF
                     IHLARYIEYSTLGIGVRFDKVFGPYYAKDLQNGKITREEALELLQLLWVKFHELGLIY
                     SPMLSGIYGGVESLQAMTIGGVGPDGRDVTNEMTYLVLETAETMRTLEPSLVLRYHDG
                     TPDRLLTRATEVIRTGVGYPSFFNDRALIPTLQGWGVPLEEARDYAITGCVYLEIPGK
                     NIARRAMGALVLPKCLWWALHQGVNPKTKEPWGAPTPDPATFRNVDDLIEAYLEQVRF
                     FFGRLNKIENTCRSLYQEYLPRPFYSAVLEGCIERGQDTRRWAYPSMVHDFCVIIGPT
                     NVADSLAAIQKVVFEDRKITMARLIEALDHNWEGFEEVRQMMLRAPKYGNDDDYADEI
                     AARVHHRTSEVLAEFTDRFGKPCRGDGSGVSGTYGLALHTPATPDGRRTGDPFSDATL
                     SPSQGRDTRGPTAVLKSAAKVDTRKTYNHLLNQKFLPSMLEGDMKPIFVSYLRTWGDL
                     GISHIQFNVIDRETLLAAQKNPERYSDLIVRVAGYSAYFVDLSKGLQDSIIARTEQRF
                     "
BASE COUNT          535 a          816 c          790 g          630 t
ORIGIN      
        1 agaggatctt gggattccgg gttggagcac caatcacagc gaagagggca ccctttgaag
       61 aagaccgagg tccggattcc cggcccgtca tggatgctga aacgctggat gttgaagatg
      121 attcctcgat ccgaattgcg gatttcggat tgcggatttc ggattggatt cccttcgttt
      181 ggcattttgc accctggagg cttatctctc gggtttgaat tcttgaaaat aagagagggg
      241 catgaagttc agaaccgctg ctccgtccgg gcgatgatgg aatcctgcag acccttgctc
      301 aggtccacaa aataggcgct gtagccggcg accctcacga tcaggtccga atacctctcc
      361 gggtttttct gggccgccag aagggtctct cgatcaatca cattgaattg gatgtggctg
      421 atccccaggt ccccccaggt cctgaggtag ctgacaaaga tcggcttcat atcgccttcc
      481 agcattgagg gaagaaattt ctggttaaga aggtgattat aggttttgcg cgtgtcaacc
      541 ttggccgctg atttcagcac cgctgtcggg cctcgcgtat ctctcccctg ggacggagag
      601 agggtggcgt cggagaaggg gtctccggtt cggcgcccgt ccggggtggc cggcgtgtgc
      661 aaagccaggc cgtaggtccc cgatactccg cttccatccc cccggcaggg tttcccgaaa
      721 cgatccgtga actcggccag gacctctgaa gtccggtggt gaacccgggc ggcgatctca
      781 tcggcgtagt catcgtcatt gccgtacttg ggcgcccgga gcatcatttg gcggacctct
      841 tcgaaacctt cccagttgtg gtccagggct tcgatgagcc gggccatggt gatcttccgg
      901 tcttcaaaga ccactttttg gattgccgcc agggagtcgg ccacgttggt cgggccgatg
      961 atcacgcaga aatcgtgcac catggacggg taggcccacc tcctggtgtc ctgccccctc
     1021 tcgatgcagc cttccagcac ggccgagtaa aagggccggg gaaggtactc ctggtagagt
     1081 gagcggcagg tgttttcaat tttgttgagc cgcccgaaga agaaccggac ctgctccagg
     1141 taggcttcta tcagatcatc aacatttcga aaggttgcgg gatccggcgt gggggcgccc
     1201 cacggttctt tcgttttcgg attgaccccc tgatggagcg cccaccacag gcatttggga
     1261 agaaccagcg ctcccatggc ccggcgggcg atgttcttcc cgggaatctc cagatagacg
     1321 cagccggtaa tcgcgtagtc ccgggcctcc tccaggggga ctccccatcc ctgcaaggtt
     1381 ggaatgaggg cccggtcatt aaagaaagaa ggatagccca cccccgttcg aatgacttcc
     1441 gtcgcccggg taagaagtcg atccggagtc ccgtcgtggt accgcagaac caggctgggc
     1501 tccagcgttc tcatggtttc ggcggtttcc agaaccaggt aggtcatctc gttggtcacg
     1561 tctctgccgt ccgggcccac gccgccgatg gtcatggcct gcaacgactc cacgcccccg
     1621 taaatccccg agagcatggg ggaatagatc agccccagct catggaattt cacccagagg
     1681 agctgaagga gctcaagggc ttcctcccgg gtgatctttc cgttctgcag gtccttcgcg
     1741 taatagggcc cgaagacttt gtcgaagcgc actccgatgc ccagggtgga gtattcgatg
     1801 tacctcgcca gatggataaa gaaaaaggat tggagggcct cgaggagtga ccggggagga
     1861 ttttccggca cccaatcgca gaccgccgcg atctcctcca gccttctccg atcttcttcc
     1921 tggtttgccc gggcagccat ctcccgggcc atccgggcgt agcgatgggc gaagttcacc
     1981 actgcttcca gggcgatgac cgccgcctgg agaaattcct tctgctccag gtaattgagg
     2041 ggaacctccc ggtcgagcgc ctgaaggcgg tcttgggcct gccggatgag tccttttacc
     2101 cccaccttaa aaattttttc ataatcgggg atccccagct ccgaccagtg ggaccagaga
     2161 aaggtcccct cgtactgcca gtacttcagg aggtccccgg tgaagagctc ctgctggcgg
     2221 tcgctcatcg atttccctct ccagtactcg atgagtccgg ctaattcagc ccgaccggca
     2281 tcatccagca aggtctggcc ttcctggccg ctgaccaccc gttggaccga cctccagttc
     2341 atatcgatgc cgaaataaag gccttgaggg gtggaggcat tactcccgac gattttttcc
     2401 caatcctgaa tgtacaggtc catctggttg aggaccttgg caaagcctct ggccctccgt
     2461 aggaccatgg gctggccgtc ggtttctttg aaggactcgg tcagcaggcg ggaccgctgc
     2521 aaatccagct tgatccccgg cctatacatt ccccgggaag cttcttcttc gtggtatccc
     2581 cgccggacat tccggaccgc cttgatgacc agcctgctct tgatgtcctc caatttctcc
     2641 ttcattcctg catctccttg aattctggct ttgttcgaaa gtgggtgatt cgtcgaaggt
     2701 atgattcata taataacttt gaaatctggc ggaaactcct gatttgccaa cccgtctctg
     2761 aaatcccccc c
//
LOCUS       DASPRR010000469        12993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_922963, whole genome shotgun sequence.
ACCESSION   DASPRR010000469 DASPRR010000000
VERSION     DASPRR010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922963"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VEL68_17165"
     CDS             <1..661
                     /locus_tag="VEL68_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37757.1"
                     /translation="DMSDGFRDFIRQAKPLRLKDPLAETLGAFTDEGAVLTYTFTDAV
                     KLAGHACPTVTGAYLACQKALERLYPTEIPVRGEIAITVYGEPDEGVYGVMAQVFNLL
                     TGAAPATGFRGLGPKFKRKDLLKFDPRKPDPRALCFEFKRTDTGRSVLVKFHPHQIPF
                     SEEKGRRMGELLEKVIWEAAREEEKREFQELWTEKVKDMLLEQKDIDRWLTVEERRNQ
                     V"
     gene            658..1887
                     /locus_tag="VEL68_17170"
     CDS             658..1887
                     /locus_tag="VEL68_17170"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HXZ37758.1"
                     /translation="MNAVKTIDVKGLAHVEREGLIFPGIESLQPQETARIVTEFNPVP
                     LVYLLNSREGLRVAYEKEGPDEWVLQVTRTAPNQAQALDKEQLRALLQELKEGAVSDR
                     TKEKARSLLQAVDAKTLGVLEQELIREGVPHETIRKSLCDIHLDMMKDSLVAKRQEVS
                     APHPVHTLMEEHKVIVKSLRELALLLERLKGVHSFAELGADEERLKEIAHHLVEAESH
                     HQREEDALFPALEKHDITEPPQIMKMDHVEFRKRKRALYQIAQNPPDTDFFAFKAKVT
                     ELGEYIARELESHIFKEDNILYQIALQVLTPEDWEKVKKECDKIGYCCFTPEDQKKEE
                     GIMVELDLRSIMPFERHELIFQKWDGLKPGETLKITNDHDPKPLRYQFEAEYKNQFEW
                     EYVQKGPKDWIVKIKRI"
     gene            2076..2219
                     /locus_tag="VEL68_17175"
     CDS             2076..2219
                     /locus_tag="VEL68_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37759.1"
                     /translation="MDKARSTLAEKLTPAALDDLAARYEASRRAGLSLLKWAETVRTR
                     RAA"
     gene            complement(2549..3220)
                     /locus_tag="VEL68_17180"
     CDS             complement(2549..3220)
                     /locus_tag="VEL68_17180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ37760.1"
                     /translation="MGKKDKALKVMALMTQMHPREADLKKEISGLEHLMKLKDREVGF
                     TTPRKAVNPEESFGEKRRETCFDLGAELEMVEPEGIGGPKEIEISKKINGLKEILRKL
                     KEDSGPDLADPNWNYNLGVACRELGFFEDAIEHLRVSYEKRQKPFDAAYLLGLCFKEK
                     VMWEEALQAFEKALSVDGISHGNILSVRYEMGLIFKEQGKTEEALELLRKILGSRSGI
                     SGSKR"
     gene            3982..5202
                     /locus_tag="VEL68_17185"
     CDS             3982..5202
                     /locus_tag="VEL68_17185"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HXZ37761.1"
                     /translation="MAEEFGKEQKKDIMKDIIRKLHEGLSLEQAKNRFEKEIGNVTST
                     EIAEIEQGLMNEGISPDEIKKFCNVHALLFQSALEKSMDKEESPAHPVYLMRQDNREI
                     ERITGALKELAQNGQKYRPAQFKQKVGELLGKLKNLDIHYTWKEQILFPYLEKYEFYG
                     PSKVMWGKDDEVRALLKQSLTGIETLKEEKELKEYVAKTLNPLIEEVNGMIFKEEKIL
                     FPASLEKLKVQDWVEVLKETENVGYGFIQKPKETYSLIEELRRAVTEEPKAEEQTITL
                     PTGSFKLEELLGVLNTLPVDITFIDRDDAVRYFSEGKERIFLRTKSIIGRRVQNCHPP
                     KSVDVVEKILQSFKEGKRSSVDFWILYQGKHVYIQYFAVRDRQGNYLGTLEVSQDIAG
                     IKKIEGEKRLLDFS"
     gene            5507..6211
                     /locus_tag="VEL68_17190"
     CDS             5507..6211
                     /locus_tag="VEL68_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXZ37762.1"
                     /translation="MALTKRAAFWIFSLGTLSSTVLFLALTIDTHRQVETLTHADKLS
                     DQVVAGKRVWEKYNCNDCHTILGFGGYYAPDMTKAYKRLGEVGIRERVTKPEVIFANS
                     WRKMPQQNLSPDEVEKLIHFFKWVGDIDTHGWPPQDSEKRSSSSVRRLIASVGMSPGA
                     ALFKEKGCLGCHRIGEAGSNVGPALDKVGSKFDKETLAQYAMDPKKVNPQSSMPAQDV
                     QPQEGRQIAEFLAGLK"
     gene            6226..7587
                     /locus_tag="VEL68_17195"
     CDS             6226..7587
                     /locus_tag="VEL68_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXZ37763.1"
                     /translation="MEYQSQRVALPYFALALLLFVLQVLMGLWLAINYFATLPQGLVN
                     IFPFATARAMHTNLLVLWLLLGFMGGTYYIIPVETKRELYSTKLAYVQLVILALTGVT
                     ALVGFLFGWNQGRPLLEIPTALDFVVVIGALMFLFNVGMTMWKAKNWTVIQGTLLGGL
                     TFLALLYLFGIPFYRNLTIDWYYWWWVIHLWVEGAWELVTGAIFAFVLMRITGVERQV
                     VEKWLYVEIGLFLFTGIAGTGHHYYWLGAPRYWLWIGGIFSALEPLPILLMVIDTMNH
                     VKHRKLEVKNSLVWTYAIGGAFYHLLGAGLWGFAHTLPQVNYYTHGSQVTVSHGHLAF
                     FGAYAMLNLMVFYFAMPQLKGIPNYADGRGKFGFWTMTSAMFCIGLGFGVAGILQAYL
                     ERVLGMGFMVAQEQMRLWFQVVFFCGIAFLVGVIVTVYDLLFLKPKQEASPARAAAAQ
                     PAS"
     gene            complement(7809..8360)
                     /locus_tag="VEL68_17200"
     CDS             complement(7809..8360)
                     /locus_tag="VEL68_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXZ37764.1"
                     /translation="MEALDILIDEHGIIRQFLDNLVLSLKKMEQGERPPKKFFEKAVS
                     FARDFTDKYHHFKEEHILFNRLAMKKKGKIDAEVESLKYQHERGRALIGEVAKALEGY
                     GQGREAQTLILLESLAAYISLLRQHIHKEDHVFYPMVEKEFPWDEYRGLLEVFQQEGK
                     KMGERFVEKSKRDLYEMGGLLIT"
     gene            complement(8481..8750)
                     /locus_tag="VEL68_17205"
     CDS             complement(8481..8750)
                     /locus_tag="VEL68_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37765.1"
                     /translation="MKNLTEVFLFGSLNGESSGTQALPIHCNLEQPILLPDLLDRLQI
                     PRGRVQLTMVNHRAVPPDHLILPGDRVALFPREYAIFADWRDLRL"
     gene            complement(8905..9378)
                     /locus_tag="VEL68_17210"
     CDS             complement(8905..9378)
                     /locus_tag="VEL68_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HXZ37766.1"
                     /translation="MAQFSGCPGSRMVDFREKSEHNKEEAKGKIQSQLRQWPIQLHLI
                     SPTASYYQGADVLLTADCVGYALGGFHPEFLKGKSLAIACPKLDEGQDIYQEKIKSWI
                     DDAKINTLTVLIMQVPCCMGLLNLAQQAAKGAKRKVPVKYVVAGIQGEILKEEWA"
     gene            complement(9382..10173)
                     /locus_tag="VEL68_17215"
     CDS             complement(9382..10173)
                     /locus_tag="VEL68_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXZ37767.1"
                     /translation="MEAQLNRSIKEVIKDFPPVEKILNEYGIGCGPCAVGLCLLKDIV
                     SIHNLPADQEQEMMGRISQVIHPGQKVQIPRVERKPEARPKELRYSPPMKKLVDEHVL
                     IKKWLALIPEILPRLDLESEEGRQLIRDGVDFIRSYADGFHHAKEEDILFKFFDENLD
                     MVKVMREDHKTARNHVKAIVEGLEGKDRQTVVHHLSAYRDLLSQHIKREDEIMLPWMD
                     NQLSVSQVGGIFSKFLEVEERMDSYQKKYEDFISRVERKYRKKEE"
     gene            complement(10254..11018)
                     /locus_tag="VEL68_17220"
     CDS             complement(10254..11018)
                     /locus_tag="VEL68_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ37768.1"
                     /translation="MKIKRKIIQIDEDKCDGCGLCVPSCAEGALQIVNGKAKIVADMF
                     CDGLGACLGECPNDALHVIERVADDFDEKAVEEHLKDSSAAHGAHPQAHGPAAAGCPS
                     ARLQTFSLASPCQQANQPASYAGSPSALTHWPVQIRLVPPTAPFLKGADLLVAADCTP
                     IAYPNFHRDFLNGKVVMMGCPKFDETDAYVEKFAEVFKVSGVKSVSVVTMEVPCCQGL
                     PMIVRKGLEISKKKIPLEQIILSTRGEVLKTQKLVA"
     gene            complement(11044..11796)
                     /locus_tag="VEL68_17225"
     CDS             complement(11044..11796)
                     /locus_tag="VEL68_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXZ37769.1"
                     /translation="MRCPGQDSRYWKPEAVYNVPCAKCGSLIEFFKDEPTRRCKKCGH
                     KMVNPKMDFGCAAYCKYAEQCLGDLPPELIAQKQELLKDRVAIEMKHFFKQDFKRIGH
                     ATKVARYAEQIVKEEKGEPAIVLTAAYLHDIGIKEAEARYGSNEAKYQEELGPPIARE
                     ILTKLGARPEMIEEVCDIVGHHHHPRPEETANFKILYDADMIMNLEETQQEKKIPREK
                     LVAMIERNFLTASGRELAKKTFLATEGTEKSV"
     gene            complement(12031..12813)
                     /locus_tag="VEL68_17230"
     CDS             complement(12031..12813)
                     /locus_tag="VEL68_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ37770.1"
                     /translation="MKWTKEAEEAVGKVPFFVRKRVRKRVEEEAARDGAGQVTLGHVK
                     TCQQRFLNRMEEEVMGFQVETCFGPSGCPNRAVAYNDLAKKVEGILAKRNLKDFLRSR
                     VGGPLKLHHEFRVSISDCPNACSRPQISDIGLIGARRPQISSEECIQCGACVEACREG
                     AIVLAGSWPVIDWPKCVFCGQCITACPTRTLQEEKRGFRIQVGGKLGRHPRLAEELPG
                     IHSPEETLETIDGCLDHYQKHCRHGARFGEILERTGTKGLVP"
BASE COUNT         3025 a         3456 c         3447 g         3065 t
ORIGIN      
        1 agacatgagc gatggattcc gcgatttcat ccggcaagcc aagcccctgc ggctcaagga
       61 ccccctggcc gaaaccctgg gggcctttac cgacgagggg gcggttttga cctatacgtt
      121 caccgatgcg gtcaagctgg ccggccatgc ctgcccgaca gtgacgggag cttacctggc
      181 ctgccaaaaa gccctggaga gactctatcc cacggaaatc cccgtacgag gggagattgc
      241 catcaccgtc tacggcgaac cggatgaagg agtgtacggg gttatggccc aggttttcaa
      301 tttgcttacc ggagccgctc cggccaccgg ctttcgcggc ctgggaccca agtttaaaag
      361 aaaggacctg ttgaagttcg atccccgcaa acccgatccc cgggccctgt gtttcgaatt
      421 caaaaggacg gacaccggca ggtcggtcct ggtcaaattc catccccacc agattccttt
      481 cagcgaagaa aaaggcaggc gcatgggaga gctcctcgaa aaggtaatct gggaagcggc
      541 ccgggaagaa gagaagaggg aattccagga actctggacg gaaaaagtaa aagatatgct
      601 cttggaacag aaggatatcg accggtggct gactgtcgaa gaaaggagga atcaggtatg
      661 aacgccgtaa aaacgatcga tgtaaaaggg ctcgcccatg tggaaaggga gggtttgatt
      721 ttcccgggca ttgaatccct ccagccccag gagaccgcga gaatcgtgac cgaattcaac
      781 cccgtgcccc tcgtgtatct cctgaattcc cgggaaggcc ttcgggtggc ctacgaaaag
      841 gagggtccgg atgaatgggt tctgcaggtg acccgcaccg cgcccaacca ggcccaggcg
      901 ctcgataagg aacagctaag agcgctgctt caggaactga aggagggcgc cgtctccgac
      961 cggaccaagg agaaagccag gtcccttctc caggcggtgg acgccaagac cctgggggtt
     1021 ctcgagcagg agctgatccg cgaaggggtt ccccatgaaa cgattcgcaa aagcctgtgt
     1081 gatatccacc tggacatgat gaaggacagc ctggtggcca agaggcagga agtatcggcc
     1141 ccccatccgg ttcataccct gatggaggag cacaaagtca tcgtgaaaag cctgagggaa
     1201 ttggcccttc tattggaaag gctcaagggg gtccacagtt ttgccgagct gggagcggac
     1261 gaggaaagac tgaaggagat tgcccaccac ctggtggagg ccgaaagcca ccatcagcgc
     1321 gaggaggatg cgctttttcc ggctttggaa aagcacgaca taaccgagcc cccccagatc
     1381 atgaagatgg atcacgtgga attccgcaaa aggaagcggg ctctctacca gattgcccag
     1441 aaccccccgg ataccgattt cttcgccttc aaggccaagg taaccgaact gggagaatac
     1501 attgcccggg agctggaaag ccacattttc aaggaagaca acatcctcta ccaaatcgcc
     1561 ctccaggtcc tgaccccgga ggactgggag aaggtcaaga aggaatgcga caaaatcggg
     1621 tactgctgct tcacccccga agatcaaaag aaggaggaag gaataatggt tgagctggac
     1681 ctgcgatcga tcatgccttt tgagaggcac gaactcatct ttcagaaatg ggacggcttg
     1741 aagccaggcg agacgctaaa gatcaccaac gaccacgacc ccaagccctt gcggtaccaa
     1801 ttcgaggcgg aatacaagaa tcaattcgaa tgggaatacg tgcagaaagg cccgaaggac
     1861 tggatcgtga agatcaagag gatttaaggg aaagagttct cttcgacatg gccatgcgga
     1921 gagaattgaa cgcggcatga tactttctga tccgagagga agaaaagagg agaaaacagg
     1981 gcgaccaaac tggatccgat tgcggagttc cggcaggatc accggaaagg gagagacctc
     2041 ttgctcagtc tggcgcaagc ctcgagaaag gacaattgga taaggcaagg agcaccctag
     2101 cggaaaaatt aacccccgcc gccctggacg atctggccgc gaggtatgaa gcctccaggc
     2161 gggccggact ctccctgctc aagtgggcgg agactgttcg tacccgaaga gcagcctgag
     2221 cagctcacct ctgaggccgt gtaatattga aaaaaggatt ctcattccct tgaagcgtgt
     2281 ccccgttgaa acgggagcga gaatccagaa caaccctcac cttcgcttcg ctcggcagaa
     2341 gcccaggagg ggagaattct actctgactc cttacgcttt cctcgttttt ccaagggaaa
     2401 ttcgcctccc tgaagaattc aacccttcca tctgcaaagt atgagaatta aactcctaaa
     2461 taagcttgcg gtttccgatt gaaagtgtgc ataacagccg gactgagcaa cggctccctt
     2521 cgctaatttc tttgtgacct tttttatctc atcttttgga tcccgaaatc cctgatcgac
     2581 tgcccaaaat ttttctcaag agttcaaggg cttcctccgt cttcccctgt tccttgaaaa
     2641 tgaggcccat ttcatacctc accgacaaga tatttccgtg agagattcca tctacgctca
     2701 aggctttctc gaacgcttgg agggcctctt cccacatgac cttctccttg aaacacaagc
     2761 ctaataaata cgctgcgtca aaaggtttct gtctcttttc ataggaaacc cgcaaatgtt
     2821 cgatcgcatc ttcaaagaac cccaattctc gacaggccac acccaagttg taattccaat
     2881 tggggtccgc caagtctggg ccactatcct ctttcagttt ccttaagatc tcttttaatc
     2941 cgttaatttt ttttgagatt tcgatttctt ttggaccgcc gattccttca ggttcgacca
     3001 tttccagctc agcacccaaa tcgaagcagg tttcccgcct tttctcgcca aaggattctt
     3061 ctggattaac ggcttttctt ggggtggtaa atccgacctc tctgtccttt aacttcatga
     3121 ggtgttctaa gcctgaaatc tcttttttca ggtcggcctc ccttgggtgc atctgggtca
     3181 ttaaggccat tactttgagg gccttatcct tcttccccaa ggatttatat tgctggaaaa
     3241 gaatcttata ctgagcgagg gcctcttgcc gaaagcccat cttctggtag atttccgcaa
     3301 gttcaagaga aacaggttcc gctgcgggat ctttcgtcgt gagcattttt tatgccccca
     3361 tgaatgtttg ctttttgctg gatttgattc tgtcgcggtc ttgctaattc cagacgaaac
     3421 cgattacaga aattagagcc acgaatgcca attttcgtac accctttatc tccgtatatt
     3481 aatttggaga tataatttgt tctttgctgg gtattggctg gttgtctgct tttccataat
     3541 tcttcgctga ttaggaatat cgccaaacga gaagtgggtt aggaggtacc cttgtccagt
     3601 cattctgcaa tcagtgtacc aaaggaaaag aactgctaag gagacaaaaa atgattaaaa
     3661 atcagaataa tacccccaaa aatggaaggc ggcttgtgcc agataagcaa ttttaaacga
     3721 acatttttgt gaagtaatcg ccgcggttgt acaaatgaag aggattgatt tggcggtccg
     3781 tcgattaatg gtgaaccaat cactagccca ttcccagtcc acaaaaggcc ttcctctttt
     3841 ctaaggagtt ttttataatt ttaagggtgg cttttttgcc cctggtttag gggagaggtc
     3901 cgattttcat cgaattttat ctggtgaggc taaagggcat tcttaaaggg ctaatataaa
     3961 atggagcgaa ggagggcgaa aatggctgaa gagttcggga aggaacagaa gaaggacatc
     4021 atgaaggata tcatccggaa gctccatgag gggctgagcc tcgaacaggc caagaaccgg
     4081 tttgagaaag agatcggcaa tgtcacctct acggagattg ccgagattga acagggtctc
     4141 atgaacgaag gaatatcgcc ggacgagatc aagaaattct gcaacgtcca cgcattgctt
     4201 tttcaatcgg ccctggaaaa gtcgatggac aaagaggagt ctccggcgca cccggtatac
     4261 ctcatgaggc aggataaccg ggagatcgag aggattaccg gggcactgaa agaattggct
     4321 cagaacggac aaaaatacag gccggcccag tttaaacaaa aagtcgggga attgctgggc
     4381 aagctgaaga acctggacat tcactacacc tggaaagaac agattctttt cccttacctg
     4441 gagaaatatg aattttacgg cccgtccaag gtcatgtggg ggaaggacga cgaggtccgt
     4501 gccttgttaa aacaaagcct gaccgggatc gaaaccttaa aagaagagaa ggaactgaaa
     4561 gagtatgtgg ccaagactct caaccccctg atcgaagagg tgaacgggat gattttcaag
     4621 gaagagaaaa tccttttccc cgcttccctg gaaaagctga aggtccagga ctgggtggaa
     4681 gtactgaagg agacggaaaa cgtggggtac gggttcatcc agaaacccaa ggaaacctat
     4741 tcgcttatcg aggaattgcg ccgagcggtg accgaggagc ccaaggccga ggagcagacc
     4801 atcaccctgc ctacgggttc gtttaagctg gaggagcttc tgggggtttt aaataccctg
     4861 cccgtggaca tcaccttcat cgacagggac gatgcagtgc ggtacttctc cgaagggaag
     4921 gaacggattt tcctccggac caaatcgatc atcggccgca gagttcagaa ctgccatcct
     4981 ccgaagagcg tcgacgtggt ggaaaaaatc ctccaatcct tcaaagaggg gaaaagaagt
     5041 tcggttgact tctggattct ttaccagggg aaacatgtct atatccagta cttcgccgtc
     5101 cgcgaccggc agggaaatta tttgggtacg ctggaggttt cccaggatat cgccgggatc
     5161 aaaaagatag agggggaaaa gaggcttctg gatttcagct gaaggccggc agaaaaagaa
     5221 aggatccccc cctcccatcc tttaatattt gctccgcttg acttaagtca attacctgaa
     5281 tatttgaacc cagggataat gggtcttaca ggtcttccct tatccttttc cccgcagccc
     5341 gcgggcagaa ggagaacagc gagaaagcgg aagaagggcg aagccttcca aagagtttag
     5401 ggatttttcg tgggaacaga aaaaaagaga gagggaggtg atgaaaggtc tcggctaatt
     5461 tttctttcgg gtatccataa aacagatcag aaaggaggtt caggatatgg ctttgacgaa
     5521 acgagcggcg ttctggattt tttcgctcgg aaccttgagc tcgacggttc tctttctggc
     5581 gctcaccatc gatactcatc gccaggtcga gaccctcacc catgcggaca agctctccga
     5641 ccaggtggtg gcggggaagc gggtgtggga gaaatacaat tgcaatgact gccatactat
     5701 tctagggttc ggcgggtatt acgccccgga catgacgaaa gcctataaac ggctggggga
     5761 agtgggcatt cgggaaaggg tgaccaagcc ggaggtcata tttgccaatt cgtggagaaa
     5821 gatgccccag cagaacctat cccccgatga ggtcgaaaag ttaattcatt tcttcaaatg
     5881 ggtcggtgac atcgatactc acggttggcc tccccaggac agtgagaaga ggtcctcttc
     5941 ctcggtgcgc aggctcatcg cctcggtggg gatgagtccg ggggccgccc tgttcaagga
     6001 aaaggggtgc ttgggatgcc accggatcgg cgaggcgggg agcaatgtgg ggccggcgct
     6061 ggacaaagta ggttcaaaat tcgataagga aaccctggcc cagtatgcca tggaccccaa
     6121 aaaggtcaat cctcaatcca gcatgccagc ccaggatgtt cagcctcagg aaggccgaca
     6181 gatcgccgaa ttcctggccg gattgaagtg aggaggagaa aaaaaatgga ataccaatcc
     6241 caaagagtcg ctctgccgta ttttgcccta gctttgttgc tgttcgtcct gcaggtgctg
     6301 atgggcctct ggctggccat caattatttt gctacccttc cccagggcct ggttaacatt
     6361 ttcccctttg ccacggccag ggccatgcat accaatctcc tggtgttgtg gctgctcctg
     6421 ggtttcatgg ggggcacgta ttatatcatc ccggtggaaa ccaagaggga gctttacagc
     6481 actaaactgg cctacgtcca gttggttatc ctggcgctca ccggggtgac cgccctggtg
     6541 ggattcctat tcgggtggaa ccaggggcgc ccccttctgg aaattcccac tgcgctcgac
     6601 ttcgtggtgg tgattggggc cctcatgttt ctgttcaacg tgggcatgac catgtggaaa
     6661 gccaaaaact ggaccgtcat ccagggaact ttgttgggag gactgacttt tctggccctg
     6721 ctgtatctct tcggcattcc cttctaccgc aacctgacca tcgattggta ttactggtgg
     6781 tgggtcatcc atctgtgggt agagggggcc tgggagctgg taactggcgc cattttcgct
     6841 ttcgtcctga tgcgcatcac cggggtggaa aggcaggtgg tggaaaagtg gctctacgtg
     6901 gaaatcggcc ttttcctttt cactgggatc gcgggaacgg gccatcacta ctactggctg
     6961 ggggctcccc gctactggct ctggatcggg gggattttca gcgccctcga accccttccc
     7021 atcctgctca tggtgatcga cacgatgaac catgtaaagc atcggaagct ggaggtcaaa
     7081 aattccctgg tctggaccta cgcgatcggg ggagccttct accacctgct cggggccggg
     7141 ctctgggggt ttgcccatac cctcccgcag gtcaattact acacccatgg aagccaggtg
     7201 acggtttccc acgggcatct ggcctttttc ggagcctacg ccatgctgaa cctcatggtc
     7261 ttttatttcg cgatgcccca gctgaaaggg attccgaatt acgccgacgg gcgggggaaa
     7321 tttggattct ggaccatgac gtcagcgatg ttctgcatcg gcctgggctt tggggtggcc
     7381 ggaatcctgc aggcctacct ggagagagtg ctgggaatgg gctttatggt cgcgcaggag
     7441 cagatgaggt tatggttcca ggtggtcttc ttttgcggga tcgcctttct ggtcggggta
     7501 atcgtgacgg tttatgacct tctcttttta aagccgaagc aagaagcctc tccggcaagg
     7561 gcggcggcag cccaaccggc ttcgtaatcc cttgcccttg aaatggtacg ctaaaggcaa
     7621 tagaagtttt aagggattta gatgtagggg cgaccagccg gtcgccccta catccaggat
     7681 aaacgcagac tcgtggagaa agaaaaggcg gtctccccgc agtagggtag gaggcattca
     7741 cgaatagccc gggcaatcgg gtccatctct caatcccgtg taggggcggg tttcaaaccc
     7801 gcccctactt aggtaattaa cagccctccc atctcatata agtcccgctt gctcttttcc
     7861 acgaatcttt cccccatctt cttcccttcc tgctgaaaga cctccagaag cccccggtat
     7921 tcatcccagg gaaattcctt ttccaccatg gggtagaaga catgatcttc cttgtgaata
     7981 tgctgccgca agagggatat gtaggcagcc agactctcca gaaggatcaa ggtttgggct
     8041 tcccgcccct gaccatatcc ctccagcgcc ttggccacct caccgatcag ggctctaccc
     8101 cgctcatgct ggtatttcag ggattccacc tcggcgtcga ttttcccctt ctttttcatg
     8161 gccaggcggt tgaagagaat atgctcttct ttgaagtgat ggtatttgtc ggtaaaatcc
     8221 cgggcgaatg aaaccgcttt ttcgaaaaat ttcttcgggg gccgttcccc ctgttccatt
     8281 ttcttcagcg aaagaaccag attatccaga aactgacgga tgatgccgtg ttcatcgatc
     8341 agaatatcca gggcttccat gagcgcactc ctttcttgaa tccagacctt ccgggatttc
     8401 ctgtccgttc cctgatccgc gccctgcctc cttccttccg tcctctcttc tcccttccgt
     8461 agcgcggacc catcacggcg ttagaggcgc aaatccctcc agtcggcaaa aatggcatat
     8521 tctctcggga aaagagccac ccgatccccg ggcaggatca aatgatccgg agggaccgca
     8581 cgatgattga ccatcgtcag ctgaacccgg ccccgtggaa tctgaagacg gtccaaaaga
     8641 tcgggaagaa gaatgggctg ctccagattg cagtggatcg gaagcgcttg agttccgctt
     8701 gactctccgt tcaaagaccc aaacaaaaag acttcggtca ggtttttcat aaaggatacc
     8761 ttgattgata atgagtagga ttacgaggaa gggcccgaag ccaaaaaaga ccctccccct
     8821 tcctgccttg ggatgccatg aagactcttc gagaggcccg gtcgggggcg gaccttctcc
     8881 cgggttgagg aaagaaggga aagcttaggc ccattcttcc ttcaggattt ctccctgaat
     8941 cccagccacc acatatttca ccgggacttt tcgcttggca cccttggccg cctgctgcgc
     9001 taggttcaag aggcccatgc aacaagggac ctgcataatc agaacagtta gggtgttgat
     9061 cttggcgtca tcgatccagg atttgatttt ctcctggtag atgtcctgcc cttcgtccag
     9121 cttggggcag gcgatggcca gggatttgcc ctttaaaaat tccgggtgaa acccccccag
     9181 agcatagcct acgcagtcgg cggttagcag cacgtccgcc ccctggtaat aagatgccgt
     9241 gggagagatt agatggagtt gaattggcca ctgccgcagc tgggactgga ttttcccctt
     9301 ggcttcttct ttattgtgtt cgcttttctc ccggaaatca accatcctgg accctgggca
     9361 accgctgaat tgagccatgg tttactcctc cttctttcga tattttctct ctacgcgaga
     9421 gatgaaatct tcatattttt tctggtagga atccatccgc tcctcaactt ccaagaattt
     9481 agagaaaatt cctcccacct gggaaacgga aagctggttg tccatccagg gcagcatgat
     9541 ctcgtcttcc cgcttgatgt gctgggacag aaggtctcgg taggcgctga gatggtgaac
     9601 cacggtctgg cggtctttgc cttccaggcc ctccacgatg gctttcacgt ggtttcgggc
     9661 cgtcttgtgg tcctcgcgca tgaccttcac catgtccagg ttctcgtcga aaaatttgaa
     9721 tagaatgtct tcttctttgg catggtggaa cccgtccgca taggagcgaa tgaaatcgac
     9781 cccgtcccgg atcagctgcc ggccttcctc ggattccagg tccagccggg ggagaatctc
     9841 ggggatcaga gccagccact tcttgatgag gacgtgttca tcgaccagct ttttcatggg
     9901 gggagagtat ctgagctcct tcggcctggc ctctggtttc ctctccaccc tgggaatctg
     9961 gaccttctgc ccggggtgga tcacctggga aatccgtccc atcatctcct gctcttgatc
    10021 agccggcagg ttgtggatgc tcacgatatc tttcaacagg cacaggccca ccgcacaggg
    10081 tccgcaacca attccatatt cattgaggat tttttccaca gggggaaaat ctttgatgac
    10141 ctctttgatg cttcggttca attgtgcttc catgtaacac ccctttccct gaagaccgaa
    10201 tatgggccga atcggcgtaa gggcgattca ggaacggccg acaaggaatt cctttaggct
    10261 acgagttttt gggtcttcaa gacctccccc cgggtgctca agatgatctg ctccagggga
    10321 atcttcttct tggagatctc caagcccttc cggacgatca taggcagccc ctggcagcag
    10381 gggacttcca tggttaccac tgagaccgac ttgaccccgg acaccttgaa gacctcggcg
    10441 aatttctcta cataggcatc ggtctcgtca aacttggggc agcccatcat cacgaccttc
    10501 ccgttcaaga aatcccgatg aaaattcggg taggcgatgg gggtacaatc ggccgccacc
    10561 aggaggtcgg ctcccttcag gaaaggcgct gtggggggaa ccaggcgaat ttgtaccggc
    10621 cagtgggtca aggccgaggg gctgccggca taggacgccg gctggttcgc ctgctggcag
    10681 ggagatgcca gggaaaaggt ctggagccgg gccgaagggc agcctgcggc cgcgggccca
    10741 tgggcctgag gatgagcccc atgggccgcg ctgctgtcct ttagatgctc ttccacggct
    10801 ttctcgtcaa agtcatccgc caccctctca attacatgga gggcgtcatt cgggcactct
    10861 cccaggcagg cccccaaacc gtcgcagaac atatcggcca caatcttggc ctttccgttc
    10921 acgatctgca gggcgccctc ggcgcaggag gggacgcaga gcccgcatcc gtcgcatttg
    10981 tcttcatcga tctgaatgat ctttcgtttg attttcattt ttttctcctt attatttgtc
    11041 tattcaaaca cttttctccg tgccctctgt ggctaaaaag gtttttttgg ccagctctcg
    11101 gccggaggcg gtcaggaaat tcctttcaat catggctact agcttttccc gggggatctt
    11161 cttctcttgc tgggtttcct ccaggttcat gatcatgtcg gcgtcataga ggattttgaa
    11221 attcgccgtc tcttcgggcc gtggatgatg gtggtgaccc acgatatcgc acacctcttc
    11281 gatcatttct ggcctggccc cgagcttagt cagaatttcc cgggcgatcg gcggccccag
    11341 ttcttcctgg tacttggcct cgttgctccc gtatctggct tccgcctctt taatcccgat
    11401 gtcatggagg tatgcagcgg ttaggacgat ggccggctct cctttttctt ccttcacgat
    11461 ctgctcggcg taacgggcaa ccttggtggc gtggccgatc cttttaaaat cctgcttgaa
    11521 aaaatgcttc atctcgatgg ccacccggtc cttgaggagt tcctgcttct gggcgattaa
    11581 ttccggggga aggtctccca agcattgctc ggcgtatttg cagtaggcgg cacaaccgaa
    11641 atccatcttg gggttgacca ttttgtggcc gcacttcttg cagcggcggg tgggctcgtc
    11701 tttgaagaac tcgatgagac tcccgcattt cgcgcaggga acgttgtaca ccgcttcggg
    11761 tttccagtat cggctgtctt gtcccgggca tctcatcttt tccctcctcc caaatgatta
    11821 accgtaaagc caaaatcttt atctgtcttt accttaaacc agaaagaaaa atgttacctt
    11881 gactcaagtc aagaaaataa taaatctata ttgggcgcgg atgaacaagg ataaacacag
    11941 aaaaacagtg ccaaaaatgc ctaaagtgca ctaaattgcc aaaagtcaaa aggctggttc
    12001 ttacctaccc tctcttctaa gtcgagacaa ttaaggcact aatcctttcg tcccagtcct
    12061 ctccagaatt tccccaaaac gcgccccatg ccggcagtgc ttctgataat ggtccaggca
    12121 gccgtcgatg gtctccaggg tttcctcggg ggaatggatc cccggcaatt cttcggccag
    12181 ccgggggtgg cgtccgagct ttcctccgac ctgaatccgg aaacctctct tttcttcctg
    12241 cagggtgcgg gtcgggcagg cagtgatgca ctggccgcag aacacgcatt tgggccaatc
    12301 gataaccggc caacttccag ccagaacgat ggcaccttcc cggcaggcct cgacgcatgc
    12361 cccgcactga atgcattctt ctgaagaaat ttggggtctg cgagccccga tcagcccgat
    12421 gtcggaaatc tggggacgcg agcaggcgtt ggggcagtcg gagatggaga cccggaactc
    12481 atggtgcaac tttaaaggtc ctccgacgcg ggacctcaag aaatccttca gattcctctt
    12541 tgccagaatc ccctcgacct tcttggccag atcgttgtag gctacagccc gattcgggca
    12601 tccactcggc ccgaagcagg tctccacctg gaatcccatc acttcctctt ccatccggtt
    12661 aaggaatcgc tgctggcagg tcttgacgtg tcccagggtg acttgcccag ccccatcgcg
    12721 ggcggcctcc tcttccactc tcttcctcac ccgcttacgg acgaagaacg ggactttgcc
    12781 taccgcctcc tccgcttcct tggtccattt catgggttac cccccaaaga attggcccaa
    12841 ggtcctagac gtcgggacca gggctccttt ttttccttta ctctttactt tataacctaa
    12901 ttttcggtca tgggccttga cataagtcaa aagatgaaga taattcccct tgaggaggca
    12961 ttccatgaaa ctcatggacc agattgctcg gat
//
LOCUS       DASPRR010000470         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2050353, whole genome shotgun sequence.
ACCESSION   DASPRR010000470 DASPRR010000000
VERSION     DASPRR010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050353"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /gene="hisD"
                     /locus_tag="VEL68_17235"
     CDS             complement(<1..1160)
                     /gene="hisD"
                     /locus_tag="VEL68_17235"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXZ37771.1"
                     /translation="MRLLGSGRGNFSRFLKSLSRRGESGFERVEVSVRKILEDLKKEG
                     DSALFRLTRKYDRWPPSSRSIRVTSVEIQKALQALSREERETLEFAARRIEGFHSLQL
                     QKSWSFAEEDGTILGQIVRPMERVGIYVPGGKAAYPSTVLMNAIPARVAGVREIIMAC
                     PAPKGYLDPAVLAAAHLAGVKAIFKMGGAQAIGAMAYGTKTVPKVDKIVGPGNIYVAA
                     AKQMVFGEVSIDSIAGPSEILIISDGSGDPSWLAADLISQAEHDEQAAAILLCTSGKF
                     AKEVRLEVGNQLARLPRKAIAEGSLKKFGAILRVKSLAEAARISNGLAPEHLELAVKD
                     PWDLLPKIENAGAVFLGHISPEPIGDYVAGPNHVLPTGGTARFSSPLGVYDFL"
     gene            complement(1165..2418)
                     /gene="murA"
                     /locus_tag="VEL68_17240"
                     /pseudo
     CDS             complement(1165..2418)
                     /gene="murA"
                     /locus_tag="VEL68_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
BASE COUNT          566 a          809 c          753 g          614 t
ORIGIN      
        1 aggaaatcgt aaactcccag gggagaagag aaccgggccg tgcccccagt tggcagaaca
       61 tggttggggc cggccacata gtcgccgatg ggctcggggg aaatatggcc caggaagacg
      121 gctccggcat tttcaatttt ggggagaagg tcccatgggt ccttcaccgc cagctccaag
      181 tgttcagggg ccagcccatt ggagattcgg gcggcttcgg ctaggctctt gaccctgagg
      241 atagctccga attttttgag ggagccttcg gcaatggctt ttcgaggcag cctagccagc
      301 tggttcccga cttccaggcg aacttctttg gcaaattttc ctgaagtgca caggaggatg
      361 gctgccgcct gttcatcatg ttcggcctga gaaatcaggt cagcagccag ccaagacggg
      421 tctccgcttc catcggaaat aatgagaatc tcgctgggac cggcgatgga gtcaatggat
      481 acttctccga agaccatttg tttggcggcg gctacataaa tatttcccgg gccgacgatc
      541 ttatccacct tgggaacggt cttggtcccg taagccatgg cgccaatcgc ctgggctccc
      601 cccatcttga aaatcgcctt cacccctgca aggtgagcgg cagcaaggac agcgggatct
      661 aaataacctt tgggcgccgg acaggccatg atgatttccc ggactcccgc caccctcgcg
      721 gggatggcgt tcatgagaac ggtggaaggg taggctgcct tcccgccagg cacatagatt
      781 cctacccgct ccatggggcg gacgatctgg cccaggatcg ttccatcctc ttcggcgaag
      841 gaccaggact tttgaagctg gagggaatga aacccttcaa tccgcctggc agcaaattcc
      901 agggtttccc tttcttctcg ggagagggcc tgcagcgcct tttggatttc tacggaagtg
      961 acccggatgc tccttgaaga aggcggccag cggtcgtatt tccgggtgag tcggaaaagg
     1021 gccgaatctc cttctttttt taaatcttcc agaatttttc tgaccgacac ctccactcgc
     1081 tcaaagcccg actctcctcg ccgggagagg cttttaagaa accgggagaa attccctctt
     1141 cccgaaccca gcagtctcat ggtcttattc ttgaaccctt cgaattctgg ccccgagctt
     1201 gttcaatttt tgatccagtt tctcatatcc gcggtccaga tggtagatgc gggaaacctc
     1261 ggttgtcccc tctgcggcca gcccggccag aaccagggag gcgctggccc gcaagtcggt
     1321 ggccatcacc ggagccccgg taagatgagc aaggccctgc acgatggcgt ttttcccttc
     1381 gatgcggatg tcggcgccca tccgccgcat ctcgctcaca tgcatgaaac ggttttcgaa
     1441 gaccgtctcg gtgattaccg aaagcccacc ggccaagcac aggagggcca tgatctgggc
     1501 ttgcatatcg gttgggaacc cgggataggg aagggttttc acgtccaccg gtttgatgtt
     1561 caccggtccg atgaccttca cgccgtcccc ctccgggaat atctccagtc ccgcttcctt
     1621 cagcttggcc accacggcct ctaggtgagc ggtatggcag ttctgcagct tcacattccc
     1681 ttgggttatg ccggcggcaa ccatgaaagt cccggcctcg atccggtccg ggatgatggt
     1741 gtgctcgatg gggcgcagct ccttgacccc ttcgatcacc accacgtccg ttcccactcc
     1801 ttcgatcttg gcgcccattt ttttcaaggc ttggcccagc tccacgacct ctggctcccg
     1861 ggcggcgttt ttcaaaactg tcctcccttc cgccagggct gccgccatca tgatgttttc
     1921 cgttccggtc accgtggaga tgtcgaaata aatctccgcg cccttcagcc ggtcggcaga
     1981 ggcttctaca tacccgtgct tcaattcgac tttggccccc atgctttcga ggcccttgag
     2041 atggaggttg atgggccggg cgccgatggc gcagcccccc gggagggaaa cccgggcccg
     2101 tttctacttg gccaccaggg gccccaggac gaggacggag gcccgcatgg ttttcaccat
     2161 attgtagggc gcctcaaagg agtgaatctc agaagcgctc actcggaccg ttccgctctc
     2221 tccttcggtc ttgaccccca tcatgcgcaa aaggcgggag atggttcgga tatcagccag
     2281 agccggcaca ttccggaggg taatccagtc ttctgctagg agggtggccg caaaaagggg
     2341 aagggcggcg tttttggccc cacttatcgg aacctccccc accagccggt acccgccttc
     2401 aatgatcatt ttttccatgg gacgactccg tcaaaacaaa taggacgcag atgaacgcag
     2461 acccacgccg ataatatcat aaaaaaggaa accgatcacg ggctaaggcg gcctcttcaa
     2521 ggtccaaaat ccaattagaa atcacaaaat ttttgtaaga tcgaacccgt aaggatgccc
     2581 gcccatgcgg acatgcagaa taccaacaca caccaccaaa ggcgcaatca attgcgaaat
     2641 ttcaaatatc tttagctgga atgcatttat tattggctgc ttgggtctgg ggattttgtc
     2701 gtctccgggc tttgaaaacg cgggggatgt cgctcaggtc tt
//
LOCUS       DASPRR010000471        10735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2207634, whole genome shotgun sequence.
ACCESSION   DASPRR010000471 DASPRR010000000
VERSION     DASPRR010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10735)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207634"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1110
                     /locus_tag="VEL68_17245"
     CDS             202..1110
                     /locus_tag="VEL68_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37772.1"
                     /translation="MEDFARATLPGSSFKRFLDSLPSFLAARDFREVVARIGKAHARG
                     RPVILGMGAHPIKVGLSPLIVHLMEKGVLQAVALNGAGIIHDFEIALAGRTSEDVASE
                     IGEGTFGMAEETGRMINEAINRGWEKGSGIGRSVGEMICRGRFPHRRLSITAAGAKLG
                     IPVTVHVALGTDIIHMHPQTRGAAIGEGSHLDFRLFAALVSRIEGGVYLNLGSAVILP
                     EVFLKALTLVRNQGYRVRHFTTVNMDFIPHYRPLTNVVRRPTLEGGQGFHLTGHHEIM
                     FPLLFAAILEEIQPGERGKRGRGEKG"
     gene            complement(1165..1977)
                     /locus_tag="VEL68_17250"
     CDS             complement(1165..1977)
                     /locus_tag="VEL68_17250"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXZ37773.1"
                     /translation="MREVQFSPPFPEEPLLRRVQVNLPFPLLLEQLKAVLASGLQPEV
                     YFSGFVLDHLASKDAEKTSRALQERKVPITFHAPFMDLNPGAADEKVREITTFRFGQV
                     LDLVPLFHPRAIVLHPGYDRWRYDDDVDLWLENSLLTWKPLVERAESASVPMALENVF
                     EEKPEILRRLLKEINSPFLGYCLDAGHRNLFSQVPLEEWVEVLGSRLMEIHLHDNHGE
                     ADDHLPLGQGNIDFSSLFSLLKKKKVQPIYTIEPHEIAHLQPSLKALEKYLG"
     gene            complement(1974..2831)
                     /locus_tag="VEL68_17255"
     CDS             complement(1974..2831)
                     /locus_tag="VEL68_17255"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HXZ37774.1"
                     /translation="MIITCPECLTKFRVDEEKIPEEGIKARCSRCRHIFAVAKPAPAD
                     ESFYARGESLVGIQGDEAYPKPSRRWFFHWKWVALILLAAVIGGGLFYLGKALTLPSY
                     STLGKYADEKAMALKKFTLNSPLLKRYLGIKDPSEGFLSLERVRGYYLENNNLNRVFV
                     IEGEAVNRWKESRSFIKVKGVLLDSKGNKVQEKEAYCGNLLSEKDLKEMNKAAIEKSL
                     SSQFGISFSNVNLQPNKAVPFMIVFMDIPSEKAPAKAASDPGGKAGDSISRLTDFTVE
                     IIGSQKGSK"
     gene            complement(2940..3446)
                     /gene="hpt"
                     /locus_tag="VEL68_17260"
     CDS             complement(2940..3446)
                     /gene="hpt"
                     /locus_tag="VEL68_17260"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXZ37775.1"
                     /translation="MKLLLREEEVQRKVRQLARKISRDLAGKEVLLVGVLKGAFIFMA
                     DLARRMRSPVKMDFVRLASYGSGTVSSGRVKITKDLETSLKGKEVLIVEDIIDTGVTL
                     QYLFRRLKARKPRSLRTVALLDKPSHRKVRFRPDYVGFTIEDLFVVGYGLDCNEEFRN
                     LPGIYVLK"
     gene            3650..4120
                     /locus_tag="VEL68_17265"
     CDS             3650..4120
                     /locus_tag="VEL68_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXZ37776.1"
                     /translation="MADIKKILFATDFSDNSKYALTFALSFAQKYEAKLYVLHVIQQP
                     SYPLGMYAEISFDAMDKFSQSISQAVDKEMQALREKDLKGIPSYECMIVHGTPFLEIL
                     RTAKEKAVDLIVVGTHGRTGLDHVLFGSTAEKVVRRAPCPVLSVRLPGKEFKMM"
     gene            4574..6184
                     /locus_tag="VEL68_17270"
     CDS             4574..6184
                     /locus_tag="VEL68_17270"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXZ37777.1"
                     /translation="MVVPWTLAQEPKAPPPAEQGRGPFKVAILPITIHSAESLEYLRE
                     GVYAMFSSRVELQGRISLIERAAVKKVVSQFSGEIDTEAAQKLGEDLGADFVIFGSLT
                     KLGDSASLDLKVLDVKGGKAPSPVYVQAGKLEEIIGQVDVLARKVDEKILGYPLSPAV
                     AVKPQEAPKQTAVAEKPAEAPKAMAAIPVLPQPSPPAPGPSRSGKETYEFSKSQSFPF
                     QIVGMSIADLDGDGKSELVLIDERNVYIYRWDKEFQLIKRIEGGRLSQNMAVDVGDIR
                     KNGKAEVFVTAMADQQFVSFVVAHQEGNFKVIAKDLKWFLRVIDWGERGKVLLGQGKG
                     KEEGLLWPIYEMAWDGKTYKDSGAAKLPRGLSLNGLCPFAHEGKTYFAYIDTDSRLKV
                     MNPAGKVIWRGKDTYGSDNSVRIKNMPTGTGPQDPDDLLFVNVRLLSQGNEVFLVKHM
                     GFVGEFFKRARTYSKSEVQRLAWTGAVLMETWKSKEIEGYMADIQFDGLNPEGDKTLV
                     LAVNLPREGLVGGERTSALMFSRLQAAN"
     gene            6357..7883
                     /locus_tag="VEL68_17275"
     CDS             6357..7883
                     /locus_tag="VEL68_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37778.1"
                     /translation="MKKYLVLFGAVLLVMALASPSLAQFKSYGHMEVGTYWLMNQGFF
                     DKDRGDDNKRGMMQRYRFYLEYGDVKTVRAVLGFEADSEAWGTASYNQGGYTGTPPSG
                     VSGNQFNFRPTRIGGPTAANGNNMGATNTDQLSVELKWAFIDFVIPNTPLSVTAGLQG
                     FFWGGARAQWLQSTDLPAVMLSANFAPHRIEAYWAKQYKGNLSTDNDSDFYGLRWLMK
                     QPGMNIEAFALYSNDRRVATETWTWAGTPGAAGVTNFTLTQSNIARDFEIPQYWIGAA
                     PSFTVGNFTIEPTGIYQGGKARKYFRNTPPSGDVDQQGYLLDLLLRYQLGPGLAFEVE
                     GFYASGDDTGKNDKKTLYTFTPGSESGWSFGNTKSIFYFGNGDFQYYGYRQNSVGGHW
                     FGRGQADYSPLAWLNLKLNYLYIGDTSKGSAAVNPLTGATNNAPYLARTDADKKEIGQ
                     EINLIGTLKIYQNLTYMVGFGYFITGDVFKGAVPGARLTGNNPGDNAWNLMTNLKYAF
                     "
     gene            complement(8282..8806)
                     /locus_tag="VEL68_17280"
     CDS             complement(8282..8806)
                     /locus_tag="VEL68_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37779.1"
                     /translation="MGKKILGGCLLLFFLTGCLAMEPIEVREKTYGKGAPVISQSFAS
                     PKIGPGLTWKVYLTASDPDGDMKNIVGIIDQPGVGTYPVSITRIKEEDRRELSGYVYL
                     NTPLDLDMLNLTLHIQIEDKAGHYGKPVSFPLTFSSRDRQEPPPQGLFKEKDLGPILI
                     QLHPILGGDSPGLF"
     gene            complement(9077..9604)
                     /locus_tag="VEL68_17285"
     CDS             complement(9077..9604)
                     /locus_tag="VEL68_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37780.1"
                     /translation="MKRKFFMGFLLFFFLTGCLAMEPIEVRERTYGKGVPVISQSFAS
                     PKIRTGLTWKVYLTASDPDGDMKNIIGEIDQPGVGTYPVSITRIKEEDRRELSGYVYL
                     NTPLDLDMLNLTLYIQIEDKAGHYSRPASFPLTFSSLARQEPPPQGLFKEKDLGPILV
                     QLHPILGGDNPGQVR"
     gene            10003..>10735
                     /locus_tag="VEL68_17290"
     CDS             10003..>10735
                     /locus_tag="VEL68_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXZ37781.1"
                     /translation="MKKHFLLAPGPTPIPPEVLQALARPIIHHRTPLYEGILQEVREG
                     LKYLFQTKNEVLIFASSGTGAMEGAVTNTLCAGDKAIVVEGGKFGERFSNICRAYGVN
                     PIVLPVEWGRAVDPAAIEKILKGDPSIKAVFAQATETSTGVLHPVKEIAAIVSRCPET
                     ILVVDGISHLGAVELPMDEWKLDVVLCGSQKALMLPPGLAFAALSDKAWRFVERSSLP
                     RFYFDFKKELKNLIKNQSAYTPAISL"
BASE COUNT         2526 a         2856 c         2915 g         2438 t
ORIGIN      
        1 gcccaaaatt cctaaaatta aaaagctgga agcatcgtct cggttattcg gatttttggc
       61 tttgggaatt attggggatt gggtgcttgg aatttggaat ttgaaatgcc cccccgacct
      121 attatgaaga gaaaaattca acccttgccc ctcgacggcg tgagaacata ccccctggcc
      181 tcccgaagga gcttggttcg ggtggaggac tttgcccgcg caacgctgcc cgggtcttcc
      241 ttcaaaagat tcctcgactc gctgccttcc tttctggcgg ctcgggattt ccgggaagtc
      301 gtcgcccgga tcggcaaagc ccatgcccgg ggaaggccgg taatcctcgg catgggagcc
      361 catcccatca aagtggggct cagccccctg atcgtccatc tgatggaaaa aggggttctt
      421 caggcggtgg ccctgaacgg ggccgggatc atccacgatt tcgagattgc cctggcgggc
      481 cggacatcgg aggacgtggc ctccgaaatc ggcgagggga cctttggaat ggctgaagaa
      541 accgggcgga tgatcaacga ggccatcaac agaggctggg agaagggttc gggcatcggc
      601 cgatcggtgg gcgaaatgat ttgcaggggc agattccccc accgccggct gagtatcacc
      661 gcggcagggg caaagctggg gattccggtg accgtgcatg tggccttggg cacggacatc
      721 atccacatgc acccccaaac ccggggggcg gccatcggtg aggggagcca cctggatttt
      781 cgcctcttcg ccgcgctggt ctccaggatc gagggaggag tttatttaaa cctcgggtcc
      841 gcggtgatcc tgcccgaggt tttcctgaag gccctgaccc tggttcgcaa ccagggctat
      901 cgagtaagac atttcaccac ggtaaacatg gattttatcc cccactaccg tcctctcacg
      961 aatgtggtcc gacggcccac ccttgaaggg ggccaagggt tccacctcac cgggcaccac
     1021 gaaatcatgt tccccctcct cttcgccgcc atcctggaag aaatccaacc gggcgaaagg
     1081 gggaaaaggg gaagaggcga aaagggataa ataccggacc agtctttccc ctcaacctct
     1141 ttggcccttt cgcctttttc ccttttaccc caagtatttt tccaaagctt tcaggctggg
     1201 ctggaggtgg gcgatctcgt gcggttcaat ggtgtagatg ggctggactt tctttttctt
     1261 caggagggaa aagagcgaag aaaaatcgat gttcccctgg ccgaggggaa gatgatcatc
     1321 cgcctcgccg tggttgtcat ggaggtgaat ttccatcagc ctcgacccga ggacttcgac
     1381 ccactcctcc agtggcactt gggaaaagag attccggtgg cccgcatcca gacaatatcc
     1441 cagaaagggg gagttgattt ccttcaggag cctcctcagg atttccggct tctcctcaaa
     1501 gacgttttcc agggccatcg ggaccgacgc cgattcggcc ctttccacca aaggtttcca
     1561 ggtcaggagg ctgttttcca gccagaggtc cacgtcgtcg tcatagcgcc agcggtcata
     1621 cccgggatga agaacaattg cccgggggtg gaaaagcgga actaggtcaa ggacctgacc
     1681 aaaccggaag gtagtgatct cccgtacctt ttcatccgcc gctcccgggt ttaggtccat
     1741 gaaaggggca tgaaaggtta tcgggacctt cctctcctgc aaggcccggg aggttttctc
     1801 cgcatctttg gaagcgaggt gatccaggac gaagccgctg aaatagacct ccggctgcaa
     1861 tccggaagcg aggaccgcct tcaactgttc cagcagcagg ggaaagggga gattgacttg
     1921 aaccctccgg agcaggggtt cctcgggaaa ggggggagag aattggactt ccctcactta
     1981 gagcctttct gggagccgat gatttccacg gtgaaatcgg taagccggga gatcgaatct
     2041 cctgcctttc ccccagggtc tgaagccgcc ttggctgggg ctttttccga ggggatgtcc
     2101 atgaatacga tcatgaaggg aaccgctttg ttgggctgga ggttgacgtt ggaaaaggag
     2161 attccgaact gggaggaaag gctcttttcg atcgccgctt tgttcatctc ctttaggtct
     2221 ttttccgaaa gaaggtttcc gcagtaagcc tctttttcct ggaccttgtt tcctttggag
     2281 tccagcagga cccccttcac cttgatgaag ctccgggact ctttccagcg gttgaccgcc
     2341 tccccctcga tgacgaagac ccggttcagg ttgttgtttt ccaggtaata cccccttacc
     2401 ctctccagag agaggaatcc ttcgctcggg tccttgattc ccaggtatct cttgaggaga
     2461 ggagaattca gggtgaactt cttcaaggcc atggccttct catcggcgta tttccccagg
     2521 gtggaataac tcgggagagt cagagccttc cccaggtaaa aaaggccccc cccgatgacc
     2581 gccgcaagga gaattaacgc cacccatttc cagtggaaga accatcggcg agagggcttg
     2641 gggtaggcct catccccctg aattccaaca agggactcgc cccgggcgta aaaggattca
     2701 tccgcaggcg ccggtttcgc cacggcgaag atatgccgac accgggagca cctcgctttg
     2761 attccctctt cggggatttt ctcttcgtcc actcggaatt tggtgaggca ttcaggacag
     2821 gtgataatca taaaggagct ttcagctgtc agctttcagc tctcagcggt cagctaacag
     2881 ccctcagccg tagttgaaaa aaagttttaa gctgaaagct gatcgctgaa aactgagagc
     2941 tattttagca cataaatccc gggaaggttg cgaaattctt cgttacaatc caacccgtac
     3001 ccgacgacaa aaaggtcctc gatggtgaat cccacgtagt cgggccggaa gcgaactttc
     3061 ctgtgggaag gtttatccag caaggctacc gtgcggagag aacggggctt gcgagccttg
     3121 agccgccgga acagatactg gagggtgacc cccgtgtcga tgatgtcctc gacgatgagg
     3181 acctccttcc ccttcaaaga ggtctctagg tccttggtta tcttcaccct ccccgaagat
     3241 acggttcctg aaccgtagct ggccagacgg acgaaatcca tctttaccgg agacctcatc
     3301 ctccgggcca agtctgccat gaagatgaag gctcccttta aaacgcccac cagcaggacc
     3361 tctttgcccg ccaagtccct ggagatcttc cgagccagct ggcggacttt tctttggacc
     3421 tcttcttccc tcaggagcag tttcatggca gggagagtat aggggatttt ttacccagtt
     3481 gtaaagccga aaattttctt tttctctttc attttcggtc ttgacaattt atgcagagcg
     3541 aataagatag aggcgggtta caggttgggg tgataccaga atccgatcct tccgggtaat
     3601 gtgcctcttt ccattccaaa atccgagcag agatgcagga ggaaagacca tggccgacat
     3661 caaaaaaatt ctgttcgcca ctgacttctc ggataactcc aagtatgccc tgaccttcgc
     3721 cctttccttc gctcaaaagt acgaagccaa gctctacgtt ctccacgtga tccagcagcc
     3781 gagttatccc ctggggatgt acgcggagat ctcctttgat gccatggata aattcagcca
     3841 aagcatctcc caggccgtag acaaagaaat gcaggctctg cgggaaaagg acctaaaggg
     3901 aattccgagc tatgaatgca tgatcgtcca cgggacccca ttcctggaga ttcttcggac
     3961 cgccaaagaa aaagcggtgg acctgatcgt ggttgggacc cacgggcgca ccgggctcga
     4021 tcacgtgctc ttcgggagta ccgcagagaa ggtcgtccgc cgggctccct gcccggttct
     4081 cagcgtaagg cttccgggga aggaatttaa aatgatgtag gctattacct atgacccatc
     4141 acatgctttc cctttgttgc ataattaaca accttcagga acttgttgtg gcctttttaa
     4201 tacaaaaata gtaggacagg ggctcgatag gaataagggg agtttggaag ggaaagcgga
     4261 taaatttctg aatatctcca gagatttttc cggttcttcc atctgctttc agttatctca
     4321 atatttccaa tgcttataag gaacaagggt tgtggattta tcgggcaaag gcgttcaata
     4381 ttaatataga tgaagtgagc agggaatttt ctccaatggc actaaaattg ctaattttta
     4441 acgagcgagt gagttcatat tggccttgaa agataaaccc tacaatcgaa aatacttgtt
     4501 tttaccttcc tcgggggtac ccaaaggaag gataagctct ctgaaaatat tcatatttct
     4561 tgtggcggga gtcttggttg ttccctggac cctggcccag gagccaaagg ctcccccgcc
     4621 agctgagcag gggcgtggtc ctttcaaagt cgccatcctc cccataacca ttcacagtgc
     4681 cgaaagtctg gagtatctga gggagggtgt ttatgccatg ttctcatccc gggtggagct
     4741 ccaaggccgg atttcgctca tagaaagagc ggcggtaaaa aaagtcgtat cccagttctc
     4801 gggggaaatc gataccgaag ccgcccagaa actgggggag gatctggggg ccgatttcgt
     4861 gattttcggc agtctgacca agctgggaga cagcgccagc ctcgacttaa aggtgttgga
     4921 cgtaaaagga ggaaaagccc cctctccggt ttacgtgcag gccggtaagc tggaagagat
     4981 catcgggcag gtggatgtcc tggcccgaaa ggtcgacgag aaaattctgg gctaccctct
     5041 tagtccggca gtggctgtaa aaccgcagga ggcgccgaag cagacagcgg ttgctgagaa
     5101 acctgctgag gcgcccaagg cgatggcggc cattccggtt cttccccagc cctccccacc
     5161 agccccgggg ccctcgcgat cagggaaaga gacctacgaa ttctcgaaga gtcagtcctt
     5221 tcccttccaa atcgtgggaa tgagcattgc agacctcgac ggggatggga aaagtgaatt
     5281 ggtcctgatc gacgagcgga atgtttacat ctaccggtgg gataaagagt ttcagctgat
     5341 caaaaggatt gaagggggca ggctcagcca gaacatggcc gtcgatgtcg gggatatccg
     5401 caagaatggg aaggcagagg tctttgtgac cgccatggcg gaccagcaat ttgtctcttt
     5461 tgtggtcgcc catcaggagg gaaattttaa ggttatcgcc aaagacctca aatggttcct
     5521 ccgagtcatc gattgggggg aaaggggaaa agtcctcttg ggccagggca agggaaaaga
     5581 agaagggttg ttgtggccca tctacgaaat ggcgtgggac ggaaagacct acaaagattc
     5641 gggcgcggcg aagcttcccc gggggttgag cctcaatggg ctttgcccct tcgctcacga
     5701 ggggaaaacg tattttgcct atatcgacac agattccagg ctgaaggtga tgaaccccgc
     5761 gggaaaggtc atctggagag gaaaagacac ctacggctcg gataacagcg tccggataaa
     5821 gaacatgccc acgggcaccg ggcctcaaga cccggatgat ctattattcg tcaacgtaag
     5881 gctcttgagc caagggaatg aagtcttcct tgtgaaacac atggggttcg tcggcgaatt
     5941 ctttaaacga gcccgcactt attccaagag tgaggtgcaa cggttggcct ggaccggggc
     6001 cgtgctcatg gaaacctgga aatcgaagga gattgagggg tatatggccg atattcaatt
     6061 cgacggtcta aacccggaag gggacaagac cctggtctta gccgtaaacc tccccaggga
     6121 ggggttggtt gggggggaaa ggaccagcgc cttgatgttc agtcgcctcc aggctgccaa
     6181 ttaatttttc tattgacaag ttttttaaag atcagtattt tgggacgaag aaaagtcctt
     6241 tgtcagcaca aattcccaaa gggaaggggg tgagggggga aaagaaatta cagaaacatc
     6301 gggttgagat tttaaagaat taagaactca gcatcaatcg aaaaggaggt tacagaatga
     6361 agaagtattt ggtacttttc ggggcagtgc ttctggtcat ggccttggcc tcgccatccc
     6421 tggcgcagtt caagagttat ggccatatgg aagtcggtac ttactggctc atgaatcagg
     6481 ggttcttcga caaggatcgc ggtgatgaca acaaacgcgg gatgatgcag cggtatcgtt
     6541 tctacctgga gtacggcgac gtcaagacgg tccgtgcggt cctcgggttt gaagcggaca
     6601 gcgaggcatg gggcaccgcc tcctacaacc aaggagggta caccggaaca cccccaagtg
     6661 gagtttccgg caaccaattc aacttcaggc ccaccaggat cgggggcccg actgcggcca
     6721 acggaaacaa catgggtgcc accaacacgg accaactctc cgtggagctc aagtgggcct
     6781 tcattgactt tgtaattccc aacacccccc tgtcggtaac agccggtctc cagggcttct
     6841 tctggggggg agccagggca cagtggctgc agagcaccga ccttcccgcc gtaatgctga
     6901 gcgccaattt tgccccccac cgcatcgaag cctattgggc gaaacaatac aaggggaatc
     6961 tgtccacgga taacgactcc gatttctacg gcctgagatg gctcatgaaa cagccgggca
     7021 tgaacattga agccttcgcc ctttacagca atgaccgccg tgtggcgact gaaacatgga
     7081 cgtgggctgg gactcctggt gccgcagggg taaccaattt tactttaacc cagtcgaata
     7141 ttgctcggga ttttgagata ccgcagtatt ggatcggcgc tgccccgagc ttcaccgtcg
     7201 gcaatttcac catcgagccc accgggatct accagggcgg aaaagcccgg aaatacttcc
     7261 ggaatacccc tccctcaggg gatgtggatc aacaaggcta tctgcttgac ctgctgctga
     7321 gataccagct tgggcccggc cttgcgttcg aggttgaagg gttctacgcc tccggagacg
     7381 acaccgggaa gaacgacaaa aagaccttgt acactttcac gcccgggtcg gaaagcggct
     7441 ggtccttcgg caacacgaag tcgattttct acttcggcaa cggcgatttc caatactacg
     7501 ggtacaggca gaactcagtc ggcggccact ggtttggtcg cggccaagct gattattccc
     7561 cgcttgcctg gctgaacctg aagcttaact acctttacat tggcgacacc tccaaaggta
     7621 gcgcggcagt caaccccctg acaggggcca caaataatgc cccctatttg gcccggacag
     7681 atgccgacaa aaaagagatc ggccaggaaa tcaacctgat tgggacgctt aagatttacc
     7741 agaatctcac ctacatggtc ggattcgggt acttcatcac tggggatgtc tttaagggcg
     7801 ccgtcccggg tgcccgtctc actggtaata accccgggga caacgcctgg aacctgatga
     7861 ccaacctgaa gtacgccttc taaatcccct gtcccagaaa acccccggca ggtctcctcc
     7921 gggggttttt tcatttccgg atttcacctt taagatattg gggcacgatg catcgtgctc
     7981 ccgcgcaccc ctatgaagga aatttccttc tcacaaaaat agttgtatta atgcaaacgc
     8041 attaaatgaa tttgtcctaa atgtagggtg cgttccccga acgcaccgat actttaggcc
     8101 tgatcgggga tcaggcccta cataatgtca aaagataaaa tgcgtctaca ttagtgaggc
     8161 aagcgcctga ccggcgggat gtgtccagga ttcccaaggc ccggctatgg aagcggttca
     8221 tggggaataa accgcctccg cagggtttca tcatcgggga cataagtgag aaaagaggga
     8281 attaaaagag acccgggcta tctccgccga ggattgggtg cagttgaatc aggatgggac
     8341 ccaggtcttt ttccttaaac aatccttggg gtggcggttc ttgccgatcc cgggaggaaa
     8401 aagtcagggg aaaagagacc ggtttgccgt aatgtccggc cttatcctcg atttgaatat
     8461 gaagggttaa attaagcata tcaaggtcca agggtgtatt gaggtaaaca tatcctgaca
     8521 gctcccggcg atcttcttcc ttaatccggg taatgctcac gggataagtt cccactccgg
     8581 gttgatcgat gatgccgacg atatttttca tatccccatc cgggtccgag gccgtcaggt
     8641 acaccttcca ggtcagcccc gggccgattt tgggggaggc aaaagactgg ctgatcacgg
     8701 gcgccccttt cccatacgtc ttctcccgca cctcgatggg ttccatggct aaacagcccg
     8761 tcagaaaaaa gagcaacaga cacccaccaa ggatcttttt tcccatttta ttcccctctc
     8821 gaacctctat tcatctgcct gattgttaaa cacccaaatt cttgaaaaag agccatgact
     8881 tctctcaaca ggtccaaaaa tttttcctga aaatcgtttt ctgccttgca tttaaagggc
     8941 cctaaggaag tttcaggcgg tcagaggatt tcactcttgg tgccctttgc cccccacccc
     9001 gaccctcccc ctcggagggg ggagggaagg gtgggggtga tcttttcaaa tatttttttg
     9061 caacgtttag gttctctcat cggacctgac ccggattatc gccaccgagg attgggtgca
     9121 gttgaaccag gatgggcccc aggtcttttt ccttaaacaa tccttggggc ggcggttctt
     9181 gccgagccag ggaagaaaag gtcaggggaa aagaggccgg tctgctgtaa tgtccggcct
     9241 tatcctcgat ttgaatataa agggttaaat taagcatatc aaggtccaag ggcgtattga
     9301 ggtaaacgta ccctgacagc tccctccgat cttcttcctt aatccgcgta atgctcacgg
     9361 gataagttcc cactccgggt tgatcgattt cgccgatgat atttttcata tccccatccg
     9421 ggtccgaggc cgtcaggtaa accttccagg tcagccccgt cctgattttt ggggaggcga
     9481 aagactggct gatcacgggc acccctttcc catacgtcct ctcccgcacc tcgatgggct
     9541 ccatggctaa acagcccgtc agaaaaaaga aaagcaggaa tcccataaag aactttcttt
     9601 tcatcttttt ctcctctcag acccctttcc ctccaattcc atgcacgaag aaaacagggc
     9661 cctccgacct taatcattat atacccaaat tttatggaaa gagccatgat ttctctcatg
     9721 acggctgaca gctcttttca tccaggaatc attctttcct gcaggatcga atacgccctg
     9781 cctttttcac cctctttcac cttgacatcc cgggtcgatt ttggtatgaa atgatgcccc
     9841 gccaaattag taattaggcg attagcaatt aaatggccag atggggcaaa ggctttaggg
     9901 gaaactcccg gtggttgcca ttctggcggg cggccacaga gggccgctcc tacaacatat
     9961 ctttatgatt ccactcagcc tgggaaggga tttgaacctt ctatgaagaa acattttctc
    10021 ctggcccccg ggccgacacc gattcccccg gaggttctcc aggccctggc caggcccatc
    10081 attcaccacc gcacgcctct ttatgaaggg attctgcagg aagtgcggga ggggttgaaa
    10141 tatctattcc aaacgaagaa tgaagttctc atcttcgcct cctccggaac cggggccatg
    10201 gagggagcgg tcaccaacac tttatgcgcc ggggacaagg ccatcgtcgt ggaagggggc
    10261 aagttcggag agcggttttc caacatctgc cgcgcctacg gagtcaaccc cattgtccta
    10321 cccgtggagt ggggacgagc agtcgatccg gcggcaatcg agaaaatctt gaaaggagac
    10381 ccttcgatca aggcggtctt tgcccaggcc acagaaacct cgactggggt tctccatccg
    10441 gtcaaggaaa tcgccgccat cgtctcccgc tgcccggaaa ccatcctggt ggtggacggg
    10501 atctcccacc tgggagcggt ggagcttccc atggacgaat ggaagctgga cgtggtcctc
    10561 tgcgggtcgc agaaagccct catgcttccg cccggcctgg cctttgcggc cctcagcgac
    10621 aaggcctggc ggtttgtgga acgttcctct cttccccggt tctatttcga tttcaaaaaa
    10681 gagttgaaga atctgatcaa gaaccagagc gcctacaccc ccgccatctc tctgg
//
LOCUS       DASPRR010000472        20317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1079618, whole genome shotgun sequence.
ACCESSION   DASPRR010000472 DASPRR010000000
VERSION     DASPRR010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 20317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20317)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20317
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079618"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VEL68_17295"
     CDS             <1..807
                     /locus_tag="VEL68_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ37782.1"
                     /translation="YSDLLDQKAAAQTDVDNWRFQRDSARANLLAAEARRNLAQLDLS
                     YTEVRAPFDGRIDRRLQDPGNLVGSGESTVLAEVNQVNPLYVYFNISDADLARLIGEA
                     HWRPGNAREVQWPVFMGLLSEKDYPHQGRLDFASISLAPTTGTLLLRGIFPNPDGRML
                     PGLYARVRVPVKEGPSLLVPQEAIGYDQRGTYVLIVDEKNGVQRAGIRTGALVDHLRV
                     IEEGLTGKEWVVVKGIQKAVPGRSVTPDKADLKNPVKTSVPAAGQRKAGT"
     gene            804..3980
                     /locus_tag="VEL68_17300"
     CDS             804..3980
                     /locus_tag="VEL68_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXZ37783.1"
                     /translation="MISRFFIERPILANVLAVVTVIIGVVCFTRLPQEQYPPIVPPTI
                     QVTSRYPGASADVVAATIGVPIEQAVNGVEGSIYMSSTSSSDGSYTLTITFDVGTDLN
                     TSLALVQNLVNTSLAQLPGGVVQQGVNVRKVSTNILLVVSIFSEDNRFDEAFLSNYGI
                     INMQNPLARLPGVGQVRIFGSGPYSMRVWLDPKRLQDFGLTTQDVLNAVGDQNIQVVA
                     GQLGAPPVRSDQVWQFTINSLGRLSDVQQFQDIIVKSSTGAAARIVRLRDIARVDLGQ
                     QNYSNFANIGGLSSAQVVVFSLPEANAIAVADEVYAAVAEMSKEFPEGMKYLIRYDTT
                     KFVRGAIRSVYETLFIAGILVLVVIMVFLQSFRALLVPATTVPVTIIGASIAMVALGF
                     SVNLMTLFALVLAIGIVVDDAIVIVENSSYYIEKGLSPKDAAIQAMAELTGPVMGITT
                     ALISVFLPAAFLPGITGQIFRQFALVIAATAVISAINALTLKPTQCALWLRPRPKKPP
                     NRFYRGFNRAYEALKHPYMGVVRWMVNRAWLMLGVFFVIIGLSAWQFAREPTGFLPTE
                     DQGFAVLFARLPDGASQPRAREVSEKIGAILKDTPGVAGWVTIGGYSFLDAANVSTIS
                     STFVVYDDWSKRGAHLSQEKIVARLNRDLSVIQEALVFVLIPPPIRGLGQAGGFQMMV
                     EDRKEVGLPELQRATYELIQEANSVPDLRGLASTFTVGSPQLYLDVDRTKAQSFQVPM
                     NNVFETLRAYLGSSFVNLFNKFNQVYQVYIQADDRYRSKPEDIKNLYTKNVEGSMVPL
                     GSLLEVKRSSGAELITRYNLYPAASIFGAAAPGVSSGQAIKIMEQLAEKMLPQGVGYE
                     WTTTSYQEKRVGYQAYFIYAISITLVFLVLAALYESWASPAAVILAVPLALVGVLVAL
                     IGRSFDNNLYTQIGLVLMIALASKNAILIVEFARDLQREGMSVVDAAVEATSRRFRPI
                     MMTSFAFILGVVPLLIAGGAGAASQQAIGTVVFGGMLASTLLAIPFVPVFYVVLQRLG
                     GRRKGGEADSPSKPEDLKIRSGEDEGAK"
     gene            complement(3999..5867)
                     /locus_tag="VEL68_17305"
     CDS             complement(3999..5867)
                     /locus_tag="VEL68_17305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HXZ37784.1"
                     /translation="MRELTGWVKHGLVIYGTAASLFHLYTSGYGTFEPRVQRGLHLLF
                     LLPMVFILFPATSKSPKDHPSVWDASASLLCFLATAYTVWDSERLNFRMVGMDEVLPV
                     EVFLGALLVLLVVEGARRALSRWMALTIGVSIFYLATAPYWPGLLKFKGYSVARMVEV
                     LFLAGDEGIFGFLTGISSNILYVYIIFASFMLTAGVGEFLIQFAVWAAGWARGGAAKI
                     AVISSALYGTVSGSTVANVYATGSFTIPLMKRYGFIPRQAAAIEAIAGTGGQIMPPIM
                     GAGAFIMSEVTGIPYFKIIQAAAIPAVLYYFGLFVVVHFIALRYGMQSIPKANRPSFR
                     PILRNSYFFLPFLLIIFFLGIGYSPSKSAFFVVLIIIGLSYLDRRTWLSPRKILDTLF
                     QASVNAAIIATALAGSGMIVGILTRTGAALAFGSVLMGFSFNHLILAMILVFLVVSVL
                     GTGIPTTPSYIIAVTVGAYALGKLGVPLLAAHLFIFYYAVLSDLTPPDAITAFAAANI
                     AGSEMMSTGIEAFKLGMAGFLIPFAFVFQPALLLEGNLLAILKGTGLTALGICCLAAA
                     LVGHIWTPLNWLHRLLFITAASLLVFPTIGYELLGIGLAVALFAWAKVKKVSDPGQ"
     gene            complement(6016..6987)
                     /locus_tag="VEL68_17310"
     CDS             complement(6016..6987)
                     /locus_tag="VEL68_17310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ37785.1"
                     /translation="MKKIYLLASSALFLLSASLVHAQTLTWTAGGVGGGWYTIGGGIA
                     NLINEKSGGITVKVIPGGGTVNPRLVDKGECELGWGLPFMNAAAWNGEDPYDKKHSNL
                     RAIAGGMSMNYFHFYVGAETPFKNMDEIFRQKKALRIAISPTGTSDEWVFRKVLAHYK
                     TNYRELEAAGFKFFRGSYAEQATQFKDKNVDSVFTFLALPAAAVTEATIGRSLRILNF
                     PPDLLESLGKYGITAGKIPAGTYPKAANGNEEITAAAAGSVIITTKTLADEVAYRLAK
                     TIHENLDQFRKIHGSLAPYELKDAVTGTGLPLHSGAEKYYKEKGILK"
     gene            complement(7180..8304)
                     /locus_tag="VEL68_17315"
     CDS             complement(7180..8304)
                     /locus_tag="VEL68_17315"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HXZ37786.1"
                     /translation="MGGILLRFLLITFFPFIFGANVISLAQTKDDWARVTVPRKWSFP
                     RDFGAHPEYRTEWWYFTGNLEDSQRKRYGYQLTFFRQGVRPQLPPSPNAWDIRDIYLA
                     HFAMTDVAGNHFRAADRLSRGGPGLAGASTEGMKTWVLNWSAKMGGSVIHLDAQDPKM
                     ELSLRLVPRKPPVIHGANGFSRKGPSPGQASYYVSLTRLETNGSIKLPGSRAAVDVRG
                     VSWFDQEFGSNQLTPDQKGWDWFSLHLSDGRDLMIYFLRLKDGSVEPASSGTLVAADG
                     SARHLKLADISIAVLDRWKSPRSRGEYPSRWRIQIPSAGIDLTLTPLVADQELQTEAS
                     TAITYWEGAVAGEGRSGEVRITCEGYAELTGYAGSLGGTF"
     gene            complement(8418..10904)
                     /locus_tag="VEL68_17320"
     CDS             complement(8418..10904)
                     /locus_tag="VEL68_17320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXZ37787.1"
                     /translation="MRTVTKAFLRYLPRRRGLSILQLLGIACGVAAAAGMALSSRAAL
                     SSFSLAVEFLKGKATHSIERPAGPMEETILADLMRDPAVTSFSPVIDRTVKLNVGETV
                     RLLGIDPFLDRPLRPELSRVRLGEKRDSPGEGLSFLLREGSVLLDSELAGHLKLRPGD
                     LLPTIRGDLRVVGTFPNPSSEPLILMDIAQAQRLFRIVGRVDRVDLIVSDDAGFRSRW
                     GNGFRLQSGRQRGETLREMLQAFRLNLQALSLLALFVGIFLVYNTGMFAVVSRRKDAG
                     ILRSLGANRKEIVLAFLSEILLLGALGGALGGMAGYFLSRFLTDLVAGTISNLYFFLR
                     PSPPEWSWWVPLIGILLGCGASLLGAFFPLVELVKVDPVLALQGRTVKREQKKIARRA
                     AVAGLAFLGISGILLAASSVHVYFGFASSFALLIGASLFTGSVLIRLDPGLRWLLARI
                     TGLSGKVAAGNIRQNLGRTAVAVAAFMVALSMSIGLSSMIGSFRQSLIWWMDTQLQAE
                     IYVGRVSVMEVPEDFYEELRAMSGLGGVDPFRHVQFTYRENPIILSAVDPSVLQKYTH
                     FGWLKGGNENWEPVKKGAVIISESFYRRFGVKAGDRIVLNGAHGPAELEVAAIFYDYT
                     SEHGVVMMARSTYLKVFGDHTLNSLGIFMDADAPRRQELLAEVRERAQSRGLPVYTRD
                     QLHGSILSVFDSTFAVTRSMRLMTIIVAFFGIAGALLTLFMERQKEFGIYRAIGFSTP
                     QVAGMTLMEGLGMGLVSFLLSLGVGTALAWILIRVINLRSFNWTIFFYWEGAPYLLAA
                     AIAVLASLGAAVYPIWKVYRTYPQLQIREE"
     gene            complement(10901..11596)
                     /locus_tag="VEL68_17325"
     CDS             complement(10901..11596)
                     /locus_tag="VEL68_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37788.1"
                     /translation="MDPVIRVIDVWKNYDRDGGENSAALRGTNLEIKAGEAVALYGKS
                     GSGKSTLLNIVAGIDRPSRGRVEVEGKDLDSLGEEGRTQLRRQRIGFIFQFFNLLPTL
                     TVLENVSLSLELAGKADLQPAHRALQSVGLEGKEDRFPSELSGGEQQRVAIARALVKE
                     PVIILADEPTGNLDTQTGNQVLTLLSGRCREFGTALLMATHSPLTCKYADRMLRMVDG
                     VIVEAAFLSELPA"
     gene            complement(11902..12552)
                     /locus_tag="VEL68_17330"
     CDS             complement(11902..12552)
                     /locus_tag="VEL68_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37789.1"
                     /translation="MNTKNQEEVRKRRGKASTSRFNVFQKAVLVAGILTLFLVIGLSP
                     GMAPILSAGVVGGTLLFLLLLKNLKSSKEEIEKADPVETLPQLEEKAVEQPEPFIDRG
                     EKEIFALQEAVPAEMAQLQQGPPDKDEAGEKLEEEAHVEKEIIKEPQVLSENGKWAEI
                     QERIGLLEEKAINLEDMLMQLEEKVADLRETYLKSEPKIDLQTILTNIEERAEKIA"
     gene            complement(13004..13861)
                     /locus_tag="VEL68_17335"
     CDS             complement(13004..13861)
                     /locus_tag="VEL68_17335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXZ37790.1"
                     /translation="MEKSIVDSAEFGNTGRRVSIIGLGGEGILRTHGHEEEAREVILE
                     AVRRGITYFDSARVYAGSESYYGLVWPPNPDFRSRIFQTSKSAKRDKKGALVDLETTL
                     RNMGVDHLDLWQIHDVRTEGDLEAISGPRGSLEAFASAKAAGKVRFIGVTGHHDPSIL
                     TRAVREWPVDSVLLPVNPVEASLGGFLDETLAEAHKKGLAIIGMKVLGAGHYVLPDLD
                     VTPELLIRFALSQKITLAIVGCSTPEEVRTLTRVGRNFKPLSKKEQSEVIDRFRPYAA
                     RLAYYRGTL"
     gene            complement(13901..14884)
                     /locus_tag="VEL68_17340"
     CDS             complement(13901..14884)
                     /locus_tag="VEL68_17340"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HXZ37791.1"
                     /translation="MAAAVKIRGIYTTAFTRLLLDSGCRIADPSPEIRQRFELGPIPK
                     APDILIKDRDDHQGVDIIGEADRISRLLKTIQETLMDAVLMRFEPLGEGEAELIDELQ
                     ASRELSHARLELGGASKEKLDQLRSSVVPTLTRHHRLRILCPATLERAEKKLAKRPEL
                     KTDLEKTVFRKAILLPLRKTEGVRLEHLKIREKPIRPREGIFWEAKGTRVLIKRSFSR
                     GRYDGLDLPIEGGDYGLTEVQEGAWVVKHSYFSKDGKLKGEYYNINTPVELYAYGARY
                     IDLEIDVVRRAGKTPFIVDREDLALLVKEGKISRQMEKKAMEEAGELMKRL"
     gene            complement(14909..15955)
                     /gene="rtcA"
                     /locus_tag="VEL68_17345"
     CDS             complement(14909..15955)
                     /gene="rtcA"
                     /locus_tag="VEL68_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HXZ37792.1"
                     /translation="MLEIDGSTHSGSGTILRYSVTLAALLGVSLHMTQIRAKREKPGL
                     RPQHLQALRACSSLCEGEIRGAHVGSTEIFFHPGKRVKGGDFHWDIGTAGSTTMLAFT
                     LIPLAFFAGSPSHFSLTGGLFQDFAPSAFHMQKVLFPILRGMGAEVRIEILQPGYVPK
                     GQGKLRVEGNPLSRSLKPVQMPERGNVSEIRGISLASHLENEKVSARMADECLRLLAK
                     EGYAANMETLDDRAAAQKGAALALWAMTDRGCLLGADRAGAPGRRSESIAQFVVHSLL
                     EDLATQATTDRHLADQLILFAALAKGQTRYRIPRMTDHILSNLWLVEKILGVKTEWKE
                     NILTLEGIGFERKH"
     gene            16216..16845
                     /locus_tag="VEL68_17350"
     CDS             16216..16845
                     /locus_tag="VEL68_17350"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HXZ37793.1"
                     /translation="MSDRSLKIHWLVLLLFLTMGVGCAGVKAKIPMDPKSVDPGTAVT
                     RGEARFPLLGSPISLEKTLPSVNLVDAMTMQDTDLSKEKGAVLFLSIVPSLDTPVCEE
                     QTHYLGEEGDKLPKEVRRITISRDTPFAQKRFAKEAKLMNLQYLSDYKEGEFGKATGL
                     LMDKIMLLARSVVLVDKKGIIRYIQVVPEFTRLPDMERAFQKAMELNRE"
     gene            16944..17216
                     /locus_tag="VEL68_17355"
     CDS             16944..17216
                     /locus_tag="VEL68_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37794.1"
                     /translation="MADLALLDRVFQIILQRMVQTGQAPHYTEIAKELGFSMEEGRGL
                     MRELFAAGVPGWLFPGTDIITSFAPFNSLPTQYRITVDGQQKWFGQ"
     gene            17418..17648
                     /locus_tag="VEL68_17360"
     CDS             17418..17648
                     /locus_tag="VEL68_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37795.1"
                     /translation="MNLFRSEEHIRSWARFDPATEEGILSLSDLVKLFSGSYFRKRLE
                     PGWVSHSREYGRELVQTLKEIGKTGPFWKRPK"
     gene            17767..17952
                     /locus_tag="VEL68_17365"
     CDS             17767..17952
                     /locus_tag="VEL68_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37796.1"
                     /translation="MTRWEYLYFTDAEKDTSTLHPPTHKERLNELGRQGWELVGLVAD
                     QSGRVQKLYFKRPLAEK"
     gene            18045..18290
                     /locus_tag="VEL68_17370"
     CDS             18045..18290
                     /locus_tag="VEL68_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37797.1"
                     /translation="MINIEGKTKLSPDEVMKRLKKYFGHGGQGLQVSEENPSCITFEG
                     GGGSVNATVCTEKGKTKVNLVAQEWEYQAKEFLSQLP"
     gene            complement(18467..19726)
                     /gene="ilvA"
                     /locus_tag="VEL68_17375"
     CDS             complement(18467..19726)
                     /gene="ilvA"
                     /locus_tag="VEL68_17375"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HXZ37798.1"
                     /translation="MDDEPRITTHCMPMISKADIEAAYRNIQKDIVSTPLVYSQKLSH
                     LCGCQVLLKLENFQMTGSFKDRGALNRLLSLSPEQRGKGVIAASAGNHAQAVAYHCQR
                     LGIRSKIVMPLGTPLVKVVSTQGYGAEVILQGEMVDDAGELAIQLSKEEGLTFIHPFA
                     DPLIIAGQGTIGIEILKDELAKDLAAVLCPIGGGGLISGIATYLKETNPAVRVIGVEA
                     YACPSMKASLLEGHPVRLGKTSSLADGIAIKMVGQINFEIAQKYVDEVVTVEENEIAN
                     AVLLLLEMEKIVAEGAGAVPLAALLNRKVALQSGKVLLVISGGNIDVNILGKIITRGL
                     AMDGRIAQMTVRLKDFPGSLTAALEIIKGLQANILEVAHHRFESLAPFGYVDVSLTLE
                     TKGHAHIHEIQAALQGAGFLCEDQCRF"
     gene            complement(19752..>20317)
                     /locus_tag="VEL68_17380"
     CDS             complement(19752..>20317)
                     /locus_tag="VEL68_17380"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF268 domain-containing protein"
                     /protein_id="HXZ37799.1"
                     /translation="NFVDHQCAFASFHIQRGNPGKILDIGSYRHFILGLLAHYSVTTI
                     DVRPRNPSVGNEAIVTCDAKRLNLPDDSFDWVVSLCALEHFGLGRYGDEFDPGADQKA
                     LSEMVRVLRPGGSLTFTTTITRAAPSIAFNAHRIYSRPMLQSFCAGLTLVEEKFYSHE
                     KKGFCSFQEVTDKPRWWDVYCGCWKKA"
BASE COUNT         4291 a         5844 c         5989 g         4193 t
ORIGIN      
        1 tactccgatc ttctggacca gaaggccgcc gcccagacgg atgtggacaa ctggagattc
       61 cagagggatt cggcccgggc gaacctgctc gcggccgagg ccagacggaa cctggcgcaa
      121 ctcgacctga gctacacgga agtccgggct ccgttcgacg gccggatcga ccgccggctt
      181 caggacccgg ggaacctggt cggctcgggg gagagcacgg tcctggcaga ggtcaaccag
      241 gttaatcccc tctacgtcta cttcaacatc agcgatgcgg acctggcgcg gctgatcgga
      301 gaagcccatt ggaggcccgg aaacgcccgg gaggttcagt ggcccgtctt catgggcctc
      361 ctgagcgaga aggactatcc ccaccagggc cggcttgact ttgcctccat tagcctcgcc
      421 cccaccactg gaactctgct gctccgggga atcttcccca accccgacgg caggatgctg
      481 cccgggctct atgcgcgggt gcgagtgccc gtcaaggaag gcccctctct cctggtcccc
      541 caggaagcga tcgggtatga ccaacggggc acctacgtgc tgatcgtgga cgagaagaac
      601 ggagtacagc gggccggaat ccggaccggc gcgttggtgg accacctgcg ggtcatcgag
      661 gaaggcctca cgggaaagga gtgggtggtc gtcaagggga tccagaaggc cgttcccggg
      721 cgttccgtga cgccggataa ggcggacctg aagaacccgg tcaaaacgtc cgtgcccgcg
      781 gccggacaaa ggaaggccgg gacatgatct cccgtttctt tatcgagcgg cccatcttgg
      841 cgaacgtgct cgccgtcgtg acggtcatca tcggggtggt gtgtttcacc cggctgcctc
      901 aggaacagta ccctcccatc gtcccgccca cgattcaggt caccagccgg tatccggggg
      961 cgagcgcgga tgtggtcgcc gccacgatcg gggtgccgat cgaacaggcg gtcaacggcg
     1021 tggaaggctc gatttacatg tcctccacga gcagcagcga cggctcctac acgctcacca
     1081 tcacctttga cgtcggcacc gacctgaaca cctccctcgc cctcgttcag aacctggtca
     1141 acacctccct ggcccagctc ccgggtggcg tggtgcagca gggcgtcaac gtgcggaagg
     1201 tctccacgaa catcctgctg gtggtcagta tattctccga agacaaccgt tttgatgaag
     1261 ccttcctctc caactacggc atcatcaaca tgcagaaccc gctggcccgt cttcccgggg
     1321 tggggcaggt caggattttc gggtccggtc cttacagcat gcgggtctgg ctggacccga
     1381 agaggctgca ggatttcggc ctcacgaccc aggatgtgct gaacgccgtg ggggaccaga
     1441 acatccaggt cgtggccggg cagctgggcg ccccgccggt caggtccgat caggtctggc
     1501 agtttaccat taacagcctg ggacgactct ccgacgtcca acagtttcaa gacatcatcg
     1561 tgaagagcag cacgggggcc gcggcgcgga tcgtgcggct gcgcgatatc gcccgcgtgg
     1621 acctgggcca gcagaattat tccaacttcg ccaatatcgg cgggctcagc tccgcccagg
     1681 tcgtcgtctt ctccctcccc gaagccaacg ccatcgccgt tgccgacgag gtttacgcgg
     1741 cggtggcgga gatgagcaag gaatttcccg aggggatgaa atacctcatc cgctacgaca
     1801 ccaccaagtt cgtccgcggg gcgatccggt ccgtctacga gaccctgttc attgccggca
     1861 tcctggtcct cgtcgtcatc atggtgttcc tgcagagttt ccgggccctg ctggttccgg
     1921 ccaccaccgt cccggtgacg atcatcggcg cctcgatcgc catggtggcg ctcgggtttt
     1981 ccgtgaacct catgactctc ttcgccctgg tcctggccat cggcatcgtg gtggacgacg
     2041 ccatcgtcat cgtggagaac agctcgtatt acatcgaaaa ggggttgtcg cccaaagacg
     2101 cggccatcca ggccatggcc gagctgaccg ggccggtcat gggcattacc acggcgctga
     2161 tctcggtctt ccttccggcg gccttcttgc ccggcatcac gggccagatc tttcgccaat
     2221 tcgccctggt catagcggcc acggcggtaa tcagcgccat caacgccctg accctgaagc
     2281 ccacgcagtg cgccctgtgg ctccgtcccc ggccgaagaa gccgccaaac cggttctacc
     2341 gcggattcaa ccgggcctat gaggccttga aacatcccta catgggggtg gtccgctgga
     2401 tggtaaaccg ggcgtggctc atgctcgggg tcttcttcgt catcatcggc ctctccgcct
     2461 ggcagtttgc ccgcgagccc accgggttcc tccccacgga ggaccagggt tttgccgtcc
     2521 ttttcgcccg cctgcctgac ggggcgtcac agccgagggc cagggaagta tcggagaaga
     2581 tcggggccat cctcaaggac acccccgggg tcgcgggatg ggtgacgatc ggcgggtatt
     2641 ctttcctgga tgccgcaaac gtgtccacga tctccagcac cttcgtggtg tatgacgact
     2701 ggagcaagcg cggcgcacac ctgagccagg agaagatcgt ggcccgcctc aaccgcgacc
     2761 tttccgtcat ccaggaggcg ctggtgtttg tgctgattcc tccccccatc cgggggctgg
     2821 gacaggcggg agggtttcag atgatggtgg aagaccggaa ggaggtcggc ctcccggaat
     2881 tgcagagggc gacttacgag cttatccagg aggccaactc cgtgccggac ctgcgcggcc
     2941 tggccagcac ctttaccgtc ggcagccccc agctctacct ggacgtcgac cggacgaagg
     3001 ctcagtcctt tcaggttccc atgaataacg ttttcgagac cctccgggcc tacctgggct
     3061 cctccttcgt caacctgttc aacaaattca accaggtata ccaggtctac atccaggccg
     3121 acgatcgtta ccgctcgaag ccggaggaca tcaagaacct ctacaccaaa aatgtcgaag
     3181 gttcgatggt gcccctggga agccttctgg aggtcaagcg ttcctcgggg gcggaactga
     3241 tcacccggta caatctctac cctgcggcga gcattttcgg ggccgctgcc ccgggggtca
     3301 gctcggggca ggcgatcaag atcatggagc aattggccga gaagatgttg ccccaggggg
     3361 ttggctacga gtggacgacg acgagctacc aggaaaaacg ggtcggctac caggcctatt
     3421 tcatctacgc catttcgatc actttggttt tcctcgtgct ggccgcgctg tatgaaagct
     3481 gggcatcgcc ggccgccgtc atcctggccg tgcccctggc actggtcggg gttctcgtcg
     3541 cgctcatcgg ccggagcttc gacaacaacc tctacaccca gatcggcctg gtgttgatga
     3601 tcgccctggc cagcaagaac gccatcctca tcgtggaatt cgcccgggac ctgcagcggg
     3661 aaggcatgtc cgtggtcgac gccgcggtgg aggccacgag ccgacggttt cgtcccatca
     3721 tgatgacctc cttcgccttc atcctggggg tggtccccct cctgatcgcc gggggcgccg
     3781 gcgccgccag ccagcaggcc atcggcaccg tggtcttcgg cggcatgctc gcctccaccc
     3841 tgctcgccat tccctttgtg ccggtcttct acgtggtcct gcagcgcctg ggaggaagga
     3901 gaaaaggcgg cgaagccgac agtccgtcca agccggagga cctgaagata agaagcgggg
     3961 aggacgaggg ggcgaaatag gaaaaggggt agggcaattt attgaccagg gtcggaaact
     4021 tttttcacct tggcccaggc aaagagggct acggccaggc cgattcccaa gagttcatag
     4081 ccgatagtgg gaaagaccag gaggctggcg gcggtgatga aaaggaggcg gtgcagccag
     4141 ttgaggggag tccagatatg ccccaccagc gccgccgcaa ggcagcatat gcccagggcg
     4201 gttagacctg ttcccttcaa gatggccagc aggtttcctt ccaggagcag ggcgggctga
     4261 aaaacgaagg caaaggggat caggaagccg gccatgccca gcttgaaagc ctcgataccc
     4321 gttgacatca tctccgaccc ggcgatgttc gcggcggcaa aggcagtgat ggcgtccgga
     4381 ggggtcaggt cggagaggac ggcataatag aaaatgaaga ggtgggcagc cagcagcgga
     4441 acgcccagct tgccgagggc ataggctccg acggtcacgg cgatgatgta ggaaggagtg
     4501 gtggggatcc ctgtcccgag gacgctgacc accagaaaga ccaggatcat ggccaggatc
     4561 aggtggttga aggagaaacc catgagcacg ctgccgaaag ccagggcggc gccagtccgg
     4621 gtcaggattc ccacgatcat gccggaaccg gccagggccg tggcgatgat cgccgcgttg
     4681 accgaggcct gaaaaagggt gtccaggatt tttcgggggg atagccaggt cctgcggtcc
     4741 aggtagctca ggccgatgat gatgaggacc acgaagaagg ctgatttact gggcgagtat
     4801 ccgatcccca agaagaagat gatgagaagg aagggaagga agaaatagga attccgcagg
     4861 atcggtcgga agctcgggcg attggctttg ggaatgctct gcatcccgta gcgcagggcg
     4921 atgaaatgaa ccaccacgaa gaggccgaaa tagtagagga ccgcgggaat ggcggcggct
     4981 tggataatct tgaagtaagg gattccggtc acctccgaca tgatgaaggc gccggctccc
     5041 ataatcgggg gcatgatctg cccccccgtg ccggcgatgg cctcgatcgc cgcggcctgt
     5101 cgggggatga agccgtagcg cttcatgagg ggaatggtaa agcttcccgt agcgtagaca
     5161 ttggccacgg tgctgccgct caccgtcccg tacagggcgc tggagatgac cgcgatcttg
     5221 gccgctcccc ccctggccca gcccgcggcc cagacagcaa actggatcag gaactctccg
     5281 acgccggcag tgagcatgaa cgaggcgaat atgatgtaga cgtaaaggat gttggaggag
     5341 attccggtga ggaatccgaa aattccctcg tcgccggcaa ggaagaggac ttccaccatc
     5401 cgcgccacgg aatatccctt gaacttcaga aggcccggcc agtaaggagc cgtggccagg
     5461 taaaagatcg agaccccgat ggtcagcgcc atccaccggg agagcgcccg gcgggctcct
     5521 tcgaccacca gaaggacaag cagggcgccc agaaagacct ctacggggag cacctcatcc
     5581 atccccacca tgcggaagtt gagccgctcc gaatcccaga ccgtgtaagc cgtcgccagg
     5641 aagcagagaa gggaggcaga ggcatcccag acgctgggat ggtccttggg tgatttggag
     5701 gtggcgggga agagaatgaa gaccatggga agcaggaaga ggagatggag accccgctgg
     5761 acccggggtt cgaacgttcc gtagcccgaa gtatagagat ggaacaggga ggcggcagtt
     5821 ccgtagatga ccagcccgtg tttgacccag ccggtgagct cgcgcaagga gatttccttt
     5881 taggttcaat agaccttttt gtaggggcga cccgccgagt cgcccctacc agtttgcggg
     5941 aggggaaagg attaaaaacg cccgcacggt cccggaaggg atagggcggg cgtttcggca
     6001 tacgatcagg gatggttatt tcaggatccc tttttccttg taatattttt ccgccccgct
     6061 atggagaggc aggccggtcc cggtgaccgc gtctttcaat tcataagggg ccaggctccc
     6121 gtggattttg cggaactggt ccaggttttc atggatcgtc ttggccaggc ggtaggccac
     6181 ctcatcagcc agcgtcttgg ttgtgataat cacgctaccc gcggccgcgg cggtaatctc
     6241 ttcgttgccg ttggcagctt tcgggtaggt tcccgcggga atcttgccgg ccgtgattcc
     6301 gtacttgccc aggctttcca ggaggtccgg aggaaaattc aggatccgca ggctgcggcc
     6361 gatcgtggcc tcggtcacgg cagcggccgg gagcgccagg aacgtgaaga ccgagtcgac
     6421 atttttatcc ttgaactggg tggcctgctc ggcataggag ccgcggaaga atttaaaccc
     6481 tgcggcctcc aattcgcggt agttggtttt ataatgggcc aggaccttcc ggaagaccca
     6541 ctcgtcggaa gtgccagtag gggagatggc gatgcggagg gccttcttct gtctgaagat
     6601 ttcgtccatg ttcttgaaag gggtctctgc ccctacgtaa aagtgaaaat agttcatgct
     6661 catgccccca gcgatggcgc ggaggttgga atgctttttg tcgtaggggt cctcgccgtt
     6721 ccaggcggcg gcgttcatga aggggagccc ccatcccaac tcgcattctc ccttgtccac
     6781 cagcctgggg ttcacagtcc cgccgccggg gatgaccttt acggttatgc cccctgactt
     6841 ttcgttgatc aggttggcga ttcccccgcc gatggtgtac catcctcctc ctaccccccc
     6901 cgcggtccag gtcagggtct gggcatgaac gagggaagct gagaggagga aaagggcgga
     6961 cgaagcgaga aggtatattt tcttcataaa ggtcctcctt tgaagtccta aaaatttggg
     7021 ccgaataaat tgattattct aatggacccg aggaaaatgt aaaggggaaa aatgtggggt
     7081 tccagttgcc ggttcccggt ttccagttct cgggtaaaga taaagaacag gcaacaggtg
     7141 accgtttcat tccgcaatcc aaaatgcgca atccgcaact caaaaggttc cccctaggct
     7201 ccccgcatat ccggtcaatt ccgcgtaccc ttcgcaggtg atccggacct ccccggatcg
     7261 gccttctccc gccaccgctc cttcccagta ggtgatcgcc gtggaggcct cggtctggag
     7321 ttcctggtct gccaccagag gagtcaacgt caaatcgatt cccgccgagg gaatttggat
     7381 tctccaacgg ctgggatatt ctcctcggct gcgggggctt ttccagcggt ccaggacggc
     7441 gatcgaaata tcagcgagct tcagatgccg ggcggaccca tccgccgcca ccagggttcc
     7501 ggaggatgcc ggctcgactg aaccatcctt gagccgcagg aaatagatca tcaaatcccg
     7561 gccatcggag agatggaggc tgaaccagtc ccagcccttc tggtcggggg tgagctggtt
     7621 ggagccgaac tcctggtcaa accagctaac gccccgcaca tcgacggccg ccctggaacc
     7681 gggaagcttt atcgacccgt tggtctccag gcgggtgagg gacacataat aggaagcctg
     7741 accgggggat ggccctttcc ggctgaaccc gtttgctccg tgaatgaccg ggggtttccg
     7801 gggaacgagc cgcagactga gttccatctt tgggtcctgg gcgtcgaggt gaatgaccga
     7861 tccccccatc ttcgctgacc agttgagaac ccaggtcttc atcccctccg tgctcgcgcc
     7921 cgccaatccg ggcccgcccc tggacagccg atccgcggcc cggaagtgat tgccggctac
     7981 atcggtcatg gcaaaatggg ccaggtaaat atcccgtatg tcccaggcgt tgggagatgg
     8041 tgggagttgc gggcggactc cctggcggaa gaaagtaagc tggtagccgt agcgctttcg
     8101 ctgcgagtct tccaggttgc ccgtgaaata ccaccattcg gtccggtact cgggatgggc
     8161 cccgaagtcc cgggggaagg accacttccg ggggacggta acccgggccc agtcgtcctt
     8221 tgtttgcgcc agagaaatca cattggcccc aaaaatgaaa gggaaaaaag taatcaaaag
     8281 gaatctcaga agaatccccc ccacccccgc cctccccctc gaagggggag ggtaagggag
     8341 ggggtgagtc ggcattggcc tgatcttatt tttcaggacg ccttttgccc tcatctccta
     8401 gcctccgacc tcaaacctca ttcttccctt atctgcagtt gcggataggt ccggtaaacc
     8461 ttccagatgg gataaaccgc ggcccccagg ctggcgagaa cagcgatggc cgcggccaga
     8521 aggtaggggg ctccttccca atagaaaaag atcgtccagt tgaagctcct aaggttgatc
     8581 acccggatca ggatccaggc cagcgccgtt cccactccca gactcagcaa gaagctgacc
     8641 agccccatcc ccagcccctc catgagcgtc atcccggcca cctggggcgt ggaaaacccg
     8701 atggcccggt agattccgaa ctccttctgc cgttccatga aaagggtcag cagggccccg
     8761 gcgatcccga agaaggcgac gatgatggtc attagcctca tggaacgggt gacggcaaaa
     8821 gtggaatcga agacggaaag gatgctcccg tggagctggt cgcgggtata gacgggcagc
     8881 ccccggcttt gggctctctc ccttacttcc gcgagaagct cctggcggcg aggggcatca
     8941 gcgtccataa atatgcccag gctgttgagg gtgtggtccc cgaagacctt caggtaggtg
     9001 gaccgagcca tcatcaccac tccgtgctcg gaggtgtagt catagaagat tgcggcgacc
     9061 tccagctcgg cgggtccgtg ggctccgttc aggacgatcc ggtctcccgc cttcaccccg
     9121 aagcgccggt aaaagctctc ggagataatg accgcccctt tcttgacggg ctcccagttt
     9181 tcattccccc ccttgagcca gccgaagtgg gtgtatttct gcaacacgga cgggtccacc
     9241 gcggatagaa taatcggatt ctcccggtag gtgaactgga cgtggcggaa agggtccacg
     9301 cccccgagtc cggacatggc ccgcagttcc tcatagaaat cttcgggcac ctccattacg
     9361 gagaccctgc cgacgtaaat ttccgcctgg agctgggtgt ccatccacca gatcagggac
     9421 tggcggaagc tcccgatcat ggaactcagc ccgatggaca tggagagagc caccatgaag
     9481 gcggccacgg ccaccgccgt cctccccagg ttctgccgga tgttgcccgc ggccaccttc
     9541 ccggagaggc cggtgatcct tgccaggagc cagcgcaggc cggggtcgag ccggatcaag
     9601 acgctcccgg tgaaaaggct tgccccgatc agcagggcga aggagctggc gaagccgaag
     9661 tagacatgga cggaagaagc ggccaggaga atcccgctga ttcccaggaa cgccaagccg
     9721 gctacggccg cccgccgggc gattttcttc tgctcccttt tgaccgtgcg gccctggagg
     9781 gccaggaccg ggtcgacctt caccagctcg accaggggaa agaacgctcc gagcaggctg
     9841 gccccgcaac ccagcaggat gccgatcagg ggaacccacc acgaccattc cggcggggaa
     9901 gggcggagga aaaagtacag gttgctgatc gtgcccgcca ccaggtcagt gagaaaacga
     9961 ctcaagaaat accccgccat ccccccgaga gctcccccca gggctcccag gaggagaatc
    10021 tcggagagga aagccaggac gatctctttt cggtttgccc cgaggcttcg cagaatcccc
    10081 gcgtccttcc ggcgggacac cactgcaaac atgccggtgt tgtagaccag gaagatcccc
    10141 acgaagagcg ccagcagcga gagagcctgt aaattcaggc ggaaggcctg gagcatctcc
    10201 cgcagggtct ccccgcgctg tctgcctgac tggaggcgga aaccgttgcc ccaacgggaa
    10261 cggaatccgg cgtcatcgga gacgatcagg tccactcggt ccaccctgcc gacaatgcgg
    10321 aacagacgct gggcctgggc gatatccatc aggatcaagg gttcgctgga aggattgggg
    10381 aaagtgccga cgacccgcag gtcccccctg atggtgggga ggaggtcgcc cggacgaagc
    10441 ttcagatggc cggccaattc ggaatccaga aggacggaac cctcccggag gagaaaggag
    10501 aggccttccc cggggctatc ccttttttcg cccagccgga ctcgcgagag ttccggccgc
    10561 agcggccggt ccagaaaagg gtcgattccc agcagccgga ccgtctctcc cacgttgagt
    10621 ttcacggtcc ggtcgatgac cggagagaag gatgtcaccg ccgggtcgcg catcaggtcg
    10681 gccaggatcg tctcttccat ggggccggcg gggcgttcga tagagtgagt ggctttccct
    10741 ttaaggaact cgacggccag gctaaagctc gaaagggccg cccgcgagga gagggccatg
    10801 cccgcggcgg ccgcgacccc gcaggcaata ccgagcagct ggaggatgct gagcccgcgc
    10861 cggcggggca ggtagcgtag aaaggccttg gtgacagtcc tcatgccggg agctccgaaa
    10921 ggaaggccgc ctcgacgatg acgccatcca ccatgcgcag cattcggtcc gcgtatttgc
    10981 aggtcaaggg gctatgtgtg gccatgagga gggcggtgcc gaactcccgg cagcggccgg
    11041 agaggagcgt cagcacctgg ttgccggtct gggtgtccaa gttgcccgta ggctcgtcgg
    11101 ccaggatgat caccggctct tttaccaacg cgcgggcgat ggccacccgc tgctgctcgc
    11161 caccggaaag ctcggagggg aatcggtctt cttttccctc cagccccacg gattgcaggg
    11221 ctcgatgggc gggctggagg tccgctttcc ccgccagctc caaagaaagg gagacgtttt
    11281 cgagtacggt cagcgtgggc aggaggttga agaactggaa gatgaacccg atcctttggc
    11341 ggcgcaattg cgtccggccc tcttccccga gggagtccag gtctttcccc tccacctcaa
    11401 cgcggccccg gctgggccgg tcgatcccgg ccacgatatt cagcagggtg ctctttcccg
    11461 atccgctctt cccgtaaagg gccaccgctt ccccggcctt gatttccagg ttggttcccc
    11521 gaagggcggc cgagttctcc ccgccatccc gatcgtagtt cttccaaaca tcgatcacgc
    11581 gaataacagg gtccaaaaga tgttcctccc cggttctgat ttccagccat ctttcatgaa
    11641 ctcgtaagaa gtcgtttgaa gcgtcacccc ggcgaaagcc ggggtccaga acatcttaaa
    11701 ataaatggat tccggctttc gccggaatga cagggaaagg agctttcaga cttattacga
    11761 gatcatcatt ttcaatgtac cattagacgc gagatctgtc atcagaggca aagaaggggc
    11821 gaagagccga gagcttctaa ctccattctt tttgggggag ggaagttctg gacaagtggg
    11881 gatttgaggt tatgggcagc tttaggctat tttttctgcc ctctcctcga tgttggtaag
    11941 aatagtctgg aggtcgattt tgggttctga ctttaggtaa gtttctcgta ggtcggcgac
    12001 cttttcctcc aattgcatca gcatatcttc caggttaatg gctttttcct ccagcaaacc
    12061 gatcctttct tgaatttcgg cccattttcc attttcggaa aggacctgcg gctcctttat
    12121 gatttctttt tctacatgag cctcctcttc aagcttttct cctgcttcat ctttatcggg
    12181 aggtccttgc tgcaattgag ccatttctgc ggggactgct tcctgtaagg caaaaatttc
    12241 cttttccccc cggtcgatga agggttcagg ctgctcgacc gctttttctt ccaactgcgg
    12301 gagcgtctcc accggatcag ctttttcaat ctcttccttc gagcttttca gattcttgag
    12361 caagagcagg aataataggg ttcctccaac aacccccgcg gatagaatag gggccattcc
    12421 agggctcaaa ccgataacca aaaacagggt gaggattccc gctaccagaa ccgccttttg
    12481 aaaaacgtta aaacggctgg tgctggcttt gcctcttctt ttccttacct cttcctggtt
    12541 tttagtattc aaagcgcacc gcctccctta aattcccccc atcctggaaa ttgcggctca
    12601 actcgccacg cccacagagt gcatcggaaa gggtctcaag gacttgcggc cctagatccc
    12661 ttgaccgtcg attgccttca attcccgctc cccgattgtt gaggaaaagc tttaggagaa
    12721 cactccttgg gaatgaagca ttttcttgcc ttttgacacc gcctccagga gaggtatccc
    12781 gcgaatttct ctgatgaaac tgaggattac ccaatatatc gacattcaat aggatttaat
    12841 taaatcgaaa cctaatttca ggggaggggg cgttgtcaaa attgaggcat tgaaatgggg
    12901 ggaaatccat tttctgattg tagggccgga tcgagtagga aagcatttct taggggagca
    12961 cggttgtact attcagaatt ctgggcccgc ccgctcgaat ttatcaaagg gttccgcggt
    13021 aataggccag acgggcggca taagggcgga atctgtcgat cacttcgctc tgctccttct
    13081 tggaaagagg cttgaaattt cgtcccaccc tggttaaggt ccggacctct tcaggggtcg
    13141 aacacccgac gatggccagg gtgatcttct gcgacagagc aaagcggatc aaaagctcgg
    13201 gggttacgtc caggtccggc aaaacataat ggcctgcccc cagaaccttc ataccgatga
    13261 tcgccagtcc ctttttgtgg gcttcggcca gagtttcatc caaaaaacca cccagagacg
    13321 cctccacagg attgaccggc agcagaacgg aatctaccgg ccattcccta accgcccggg
    13381 ttaagatgga agggtcgtgg tggccggtta ccccaatgaa ccggactttc ccggctgctt
    13441 tggcagaggc aaaggcttcc agcgagcccc ggggcccgga gatggcctcc aaatccccct
    13501 cggtgcgcac gtcgtggatt tgccataaat cgagatggtc aactcccatg ttgcgaagag
    13561 tggtctccag gtcaacgagg gcacccttct tatccctttt ggctgatttg ctggtttgaa
    13621 agattctgga ccggaaatcc gggtttgggg gccagaccaa cccgtagtag ctttcgctgc
    13681 cggcatagac ccgagcggag tcgaaatagg tgattccccg acgaactgcc tccagaatca
    13741 cctctcgggc ttcttcttca tgcccatggg tgcggagaat cccttcacct cccaatccga
    13801 tgatggaaac cctcctaccg gtattcccga actcagcgga atccacgatc gatttctcca
    13861 cgcggacctc ttggcactcc cctctctctt tctccccgca tcagagcctt ttcatcagtt
    13921 ctccggcttc ttccatcgct ttcttttcca tctgtctgct gatcttccct tcctttacga
    13981 gaagtgccag gtcctcccgg tccacgataa agggagtttt tcccgcacgg cgtaccacgt
    14041 cgatttccag gtcgatatag cgggctccgt aggcatagag ttccaccggg gtattgatgt
    14101 tgtaatattc ccccttcagc ttcccatctt tcgaaaaata ggaatgcttt accacccagg
    14161 ccccttcctg tacttcggta agaccgtaat ctcctccttc gatgggcagg tcgaggccat
    14221 cataacggcc gcgggagaaa gaccgtttga tgaggactcg ggttcccttg gcctcccaga
    14281 aaatcccctc tcggggacga atgggtttct ccctgatctt aaggtgttcc agcctgaccc
    14341 cttccgtttt tcttaagggc aagagaatcg ccttccggaa caccgttttc tccaggtcgg
    14401 ttttcagctc cggacgtttc gcaagttttt tctccgctcg ctccagggtt gcagggcaga
    14461 ggatccgaag tcggtggtgt cgggtcaggg ttggcacaac ggatgaacgg agttgatcca
    14521 acttttcttt cgatgcaccg cccagctcca atcgggcatg ggagagttcc cgggaggctt
    14581 gcagctcatc gatgagttct gcttctcctt cacccagggg ctcaaaacgc atgaggacgg
    14641 catccatgag cgtttcttga atcgtcttca gcagcctgct gattcggtcc gcttccccga
    14701 tgatgtcaac tccctgatgg tcgtcccggt ctttaattaa aatatcaggg gccttgggta
    14761 tcggacccag ttcgaaacgc tgccggattt cgggggaggg gtcggcgatc cggcaccctg
    14821 aatccagaag gagcctggta aatgctgtcg tgtaaatccc ccggattttt actgccgcgg
    14881 ccatgccatc ctccgacggg tcccttcttt agtgcttcct ttcaaacccg atcccttcaa
    14941 gggttagtat attctctttc cattccgttt tcacccccag aattttctcc accagccaga
    15001 ggttggatag aatgtgatcg gtcatccggg gaatccggta gcgggtctgc cccttagcca
    15061 gggcggcgaa aagaatcaat tggtccgcca gatgtcgatc tgtggtggct tgggtcgcca
    15121 ggtcttccaa gagggaatgg accacgaatt gggcaatcga ctcagaacgc ctaccgggag
    15181 cgcctgcccg gtccgccccg agcaggcatc ccctgtcggt catcgcccag agggctaagg
    15241 cggctccttt ctgagccgct gcccggtcat ccagggtttc catgtttgcg gcgtaacctt
    15301 cttttgccag aagccgcagg cattcatcgg ccatccttgc actcaccttt tcgttttcca
    15361 ggtgagaagc cagtgagatt ccccgaatct cactcacgtt ccctcgctcg ggcatctgaa
    15421 cgggtttcaa ggaacggctc agggggttcc cctcaactcg gagcttaccc tgtcccttcg
    15481 gcacgtagcc gggctgcagg atttcgatac gtacctccgc ccccattccc cgcagaatgg
    15541 ggaagagaac tttttgcatg tgaaaagcgc tgggcgcaaa gtcctggaaa aggccgccgg
    15601 tcagggagaa atgggaaggg ctcccggcga aaaaagccag ggggatcaaa gtaaaggcca
    15661 gcatagtcgt ggagccggcg gttcctatgt cccaatgaaa atctccgcct ttcacgcgtt
    15721 tccctgggtg aaagaaaatt tccgtcgagc ccacatgggc gccccggatt tccccttcac
    15781 acaaagagga gcatgcgcgc agggcctgga ggtgttgggg tcttaatccg ggcttttctc
    15841 gtttcgcccg gatttgggtc atatggaggg aaactcccag gagagcagcg agggttacgg
    15901 aataacgaag gatggtaccg cttccggaat gggttgagcc gtctatttca agcacgaatt
    15961 cctccccgca accttcccct ctctcccggg aaatggggta ggcaagaaag tcagcaaaaa
    16021 ttcatttttt ttcgttgtgg gaactcttat aatgtaacca tcggccagta aaaagccagt
    16081 cgttttttct tctccattta taagcaaatc caaagagcgg tggtccttcg tccgcagcgg
    16141 agacaggggg taaggaggaa tccaatccgg aaggtcgttc aacaattcga aatctctcga
    16201 agaaaggaga atcaaatgtc tgacaggagt ttgaaaatcc attggcttgt ccttctattg
    16261 tttctgacta tgggtgttgg ctgcgccggg gtcaaggcga aaattcccat ggatccgaaa
    16321 tctgttgacc ccggaaccgc agtgacccgg ggtgaagccc gcttcccact tctggggtct
    16381 cctatttctc tggagaaaac acttccttcg gtgaacctcg tggatgcaat gaccatgcaa
    16441 gatacggacc tttcgaaaga aaaaggggcg gttcttttcc tcagtattgt cccttccctt
    16501 gacacgccgg tctgcgaaga gcaaacccat tatctggggg aagaagggga taagctgccc
    16561 aaagaggtcc gccggatcac catcagccgg gacacccctt tcgcccagaa acgctttgcc
    16621 aaggaggcca agcttatgaa cctccaatac ctttcggatt acaaagaagg ggaattcggc
    16681 aaggccacgg gtctcttgat ggataagatt atgctcctgg cccggtccgt cgtcctggtg
    16741 gataaaaaag ggatcatccg ctacatccag gtcgtccctg agttcacccg gctgccggat
    16801 atggaaaggg cctttcagaa ggccatggaa ttaaatagag aatagaacca agcgatcagg
    16861 gatcaggaat caggaatcgg gaaaagcgcc taccaatgaa ctaccgacga ctttacactt
    16921 agggttttta aggagagggg gaaatggctg acttggccct tttagatcgg gttttccaaa
    16981 tcatcctgca acggatggtt caaaccgggc aggctccgca ttatacggag attgccaaag
    17041 agttggggtt ttccatggag gagggacggg gtctgatgcg ggagcttttt gccgccgggg
    17101 tcccgggctg gctctttccg gggaccgata tcattacctc cttcgcccct ttcaatagtc
    17161 tgcccactca gtaccggatc acggtagatg ggcagcagaa gtggtttggc cagtgaggct
    17221 ttgagtcgct ggcagtttgc tggctttttc ccggaaaggt cgtaaggatc gacgctccct
    17281 gcctggactg cgggcttccg atgcatgcag aaatcaaaga cggaaaaata ttgcgcactg
    17341 aacccgatgg attgatgggg tatgtggccg tgcctttctg gcggtgggcg gagaacatcc
    17401 cctatgcctg aagcaccatg aatctcttcc ggtcggaaga gcacatccgc agctgggcgc
    17461 ggttcgatcc ggctaccgag gagggaatcc tctccctctc cgatctggtt aaacttttct
    17521 ccggcagcta tttccggaag agactggaac ctggatgggt ctcgcacagc cgcgagtacg
    17581 gaagggagct cgtccagacc ctgaaggaga tcggcaagac cggcccattc tggaagcggc
    17641 cgaaataacc cgggccaagg ggtagagaaa tcaggattta tttattccta taaggcccgg
    17701 cggtccctaa tttgggaaat tcctgcatct aaatattaga gaaggttatt tacgaggagg
    17761 atgacgatga ccagatggga atatctttat tttacggatg cggaaaaaga tacctccacc
    17821 ctccatccgc ccactcacaa agaaaggctg aatgaacttg gcaggcaggg atgggaactg
    17881 gtgggactgg tggccgatca atcggggcgg gtgcagaaat tgtatttcaa gcggcccttg
    17941 gcagaaaaat aagatagggg tgcgaatgga agggttcgga gggttgggga cacccttctt
    18001 aaacccctcg accccgggct cccagccttt tcgaggaggg caccatgatc aatattgaag
    18061 gaaagaccaa actgtcgccc gatgaggtca tgaagcggct gaaaaaatat ttcggtcacg
    18121 gggggcaagg tctgcaagta tctgaagaga atccctcctg tattactttc gagggagggg
    18181 gcgggtcggt aaatgccacc gtttgcacgg agaaggggaa gaccaaagtc aacctggtgg
    18241 cccaggaatg ggaataccag gccaaagaat tcctttccca acttccgtaa aacaaatgga
    18301 acgcagatga cgatgatgaa atgaatcaaa gatgttcaaa aaagagtaac cgtcaagacc
    18361 aaaccttgac caaaccttgt tgagattcaa tcaaagtttt tggaattaaa attttctgcg
    18421 aaaatctgcg tctaaatcat ttatattttt ttattcccca ttcaactcag aatctgcact
    18481 gatcttcaca gaggaacccg gctccctgca aagccgcctg gatctcatga atgtgggcat
    18541 gccccttggt ctccagggtg agggaaacgt ccacatatcc aaagggagcc agggattcaa
    18601 agcggtgatg ggccacctcc agaatattgg cctggagacc cttgatgatt tccagtgcgg
    18661 cggtcaggga cccgggaaaa tccttgaggc ggacggtcat ctgggcaatc cggccgtcca
    18721 tggccaggcc acgggtaata atcttgccca ggatgttgac gtcgatattg ccgccagaga
    18781 tgacgagcag gactttcccg ctttggaggg ccaccttgcg gttcaagagg gccgccaggg
    18841 gaacagcgcc ggccccttcg gccacgatct tttccatctc caagagcagg aggacggcgt
    18901 tggcgatctc attttcttcg accgtgacga cttcatccac atacttctgg gcgatttcga
    18961 agttgatctg ccccaccatc ttgatggcga ttccatcggc cagggaggag gtcttcccca
    19021 gcctgaccgg atggccctct aggagggagg ccttcataga gggacaagcg taggcttcca
    19081 ccccaatgac ccggaccgcc gggttggttt ccttcaggta ggtggcgatc ccggagatca
    19141 atcccccgcc cccgatcggg cagagcaccg cggccaggtc cttggccaat tcatctttta
    19201 ggatttcgat accgatcgtt ccctggccgg ctatgatcag aggatcggca aaggggtgaa
    19261 tgaaagtcaa tccctcttcc ttggaaagct ggatggcgag ctcgccggca tcatccacca
    19321 tctccccctg aagaatcact tcggctccat agccttgagt ggaaaccacc ttgacgagcg
    19381 gagttcccag gggcatgacg atcttagacc ggatcccgag ccgctggcag tggtaagcca
    19441 cggcctgggc gtggttcccg gcggaggcgg caatgacccc ttttcccctt tgttcgggag
    19501 acaagctcag cagcctattc agggcccccc ggtctttgaa agacccggtc atctggaagt
    19561 tctccagctt gaggagaacc tggcagccgc agagatggct gagcttttgg gaatagacca
    19621 agggagtgga gacgatgtcc ttctgtatat tccgataagc cgcttcaatg tccgctttgg
    19681 atatcatggg catacaatgg gtggtaatcc ggggttcgtc gtccattgac cattgttcat
    19741 tgttctttgc cctaggcctt cttccagcat ccgcagtata catcccacca ccggggtttg
    19801 tcagtaacct cttggaagga gcagaatccc ttcttttcgt ggctgtagaa cttttcctcc
    19861 accagggtga gaccggcgca gaaggattgc agcatgggac ggctgtaaat ccggtgggca
    19921 ttgaaggcga tggaaggggc ggcgcgggtg atggtggtgg tgaaggtcaa gcttccgccc
    19981 ggccgcaaaa cccggaccat ctcggatagg gccttctggt cggcgccggg gtcaaactca
    20041 tccccgtatc ttcccaggcc gaaatgttcc agagcgcaga gggagacaac ccagtcaaag
    20101 gaatcgtcgg gcaggttgag ccttttggcg tcgcaggtca cgatggcttc atttcctact
    20161 gaagggttgc gggggcggac atcgatggtt gtgacggagt aatgagccag caaacccagg
    20221 ataaagtgtc gatacgaacc gatgtccaaa atctttcccg gatttccacg ctggatgtgg
    20281 aatgaggcaa aggcgcattg gtgatcgaca aaattct
//
LOCUS       DASPRR010000473         9312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_260890, whole genome shotgun sequence.
ACCESSION   DASPRR010000473 DASPRR010000000
VERSION     DASPRR010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260890"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..233
                     /locus_tag="VEL68_17385"
     tRNA            158..233
                     /locus_tag="VEL68_17385"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:191..193,aa:Ala,seq:cgc)
     gene            650..799
                     /locus_tag="VEL68_17390"
     CDS             650..799
                     /locus_tag="VEL68_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37800.1"
                     /translation="MKKKKFSELVIVILIILFFAIPLCSLVMNESVAHWPIYSTRFLY
                     NFLFK"
     gene            1285..2322
                     /locus_tag="VEL68_17395"
     CDS             1285..2322
                     /locus_tag="VEL68_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXZ37801.1"
                     /translation="MKGVVFLGERHLELRDFPEPVPGSSEVVIEMRASGLCGSDFRAF
                     RAPRSERGDPAQLKAVGHEPCGQVIKVGPGVQTLKVGDRVIIHHYLGCGRCRWCQIGY
                     SQMCIDPKAKKLYYGRTNHGGHAERIAVHESACVPMPEGLSYEEGAACACGTGTAYDA
                     LKRLDLSGRDTFAIYGQGPVGLSATLFGVATGARVIVVEPVAYRRELAKSLGCDVAID
                     PREGDPVEAIKGLTHGEGADATLDCTGIPEPRVNTVKSARIYGRVCFVGEGGETSFDV
                     SRDIMHKQLTIHGSWTMSTIGLGEVARYVAERKLPLKKLITHRFRLEQAAEAYKVFEA
                     GQTGKVVFTWV"
     gene            2603..2863
                     /locus_tag="VEL68_17400"
     CDS             2603..2863
                     /locus_tag="VEL68_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXZ37802.1"
                     /translation="MKTLSVSEAKMKLSGLIDTVKATDEEVMITKNGRPAAVLVSPEE
                     FESLKETVAVRSDSSLMKEIKKGLRALKAKKAKVFTLEDLFE"
     gene            2887..3180
                     /locus_tag="VEL68_17405"
     CDS             2887..3180
                     /locus_tag="VEL68_17405"
                     /inference="COORDINATES: protein motif:HMM:NF027117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXZ37803.1"
                     /translation="MNKLVVPREIHDLIRKMHPDLKKKIRACLKMILSEPNAGKVLMD
                     ELSGLRSLRVSSFRIIYKIKNPKEIELVAIGPRERIYEETFRLIKESGGEREE"
     gene            3331..3552
                     /locus_tag="VEL68_17410"
     CDS             3331..3552
                     /locus_tag="VEL68_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3367..3369,aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HXZ37804.1"
                     /translation="MVKSISIEYCNDUNYYPSAASLAEAIKKKSGVEAKLIKSGGGVF
                     EVVADGKLIFSKKKEHRFPEHQEILEKIK"
     gene            3592..4785
                     /locus_tag="VEL68_17415"
     CDS             3592..4785
                     /locus_tag="VEL68_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXZ37805.1"
                     /translation="MKITDVESFIVDAGWRPWVFVKVETDKGIAGYGECSVSRSPFAV
                     AGAIEDLKPLLLGTDPRAYEMRFWEMSRQAIAEPLGIRAKAIAGVELALLDIKARALG
                     ISVVELFGGPTRDKVRLYWSHCGTTRALYPEMGTPPIRTMADISALGKEVVRRGYTAL
                     KTNIVFPGDPARVHFDGFGRGQGRAVSTDQVASNSMLKHIETLIGTFRDAVGPEVDIC
                     LDLNFNFKPESCMRIAKILEPYNLLWLEIDMYNPEALRQIKDSTNTMICTGENQIYMQ
                     DYLPYFQARCADVFMVDVAWMGFSQAKKVSDLALVFEFNVAPHNYYSHLSTYISASLC
                     AVLPNVRIMEIDVDDVPLREALVSRQPEIQDGYMKIPTGPGWGTELKEDVARQNPWHK
                     PKARW"
     gene            complement(4850..5587)
                     /locus_tag="VEL68_17420"
     CDS             complement(4850..5587)
                     /locus_tag="VEL68_17420"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HXZ37806.1"
                     /translation="MEAEIEKTLSSLRSRHINGFFAENHAEANQKILALIPAEATLAI
                     GDSTSMRQLGILPALRKRGTKILNPFEPKGEGGSAEEFRKRNYQVRRDGTLCDGFLTG
                     TNALTQDGRLVNLDATGNRVAGMFWGHRLSIVVVGKNKIVRNLDEAFQRIRNIIAPTH
                     FRIRSGELGGRKRETPCVATGECTDCRAADRGCNIFTIIEGKPTQTNLNVILVDQDLG
                     LGWDPSWPQERISKILQSYKQFVWVPV"
     gene            5762..7345
                     /locus_tag="VEL68_17425"
     CDS             5762..7345
                     /locus_tag="VEL68_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXZ37807.1"
                     /translation="MGDEKDGKDQGWLPEVEELRHRESLASQMGGPENLARHRAAGRL
                     NVRERIDRLLDKGSFHETGAITGIPTFEGDRLVKLFPSNFIMGTGRINNRKVVVGCDD
                     FTIRGGAADAAIGRKAPYAERMALDLRLPMIRLVDGTGGGGSVKYLEKMDRTYIPANP
                     AMEIMEPLLSTVPVMSAALGSVAGLGAARVVFSHFSVMPQKTAQLFVAGPPVVEAAFG
                     KRVEKEELGGSRIHTTGFGAVDNEVASEEEAFEQIRRFLSYMPNSVYELPPVKEAKDP
                     RDRREEELIGIVPKNKRLPYSVRRILELVLDQGSFFEIGAKHGRSVVTGLARLLGRPI
                     GVMAHDPKFYSGSVTAEGADKMVRFIDLCDTFHLPVVYFVDNPGFMIGVEAEKQATIR
                     FGARYIFAVYQATVPWCSIILRRVFGVAGAAHGNHSRLNLRYAWPSGDWGSLPIEGGV
                     QAAYRRQIEAAQDPQALREEIEKRLTKVTSPFRTAEIFGVEEIIDPRDTRPLLCEWIE
                     TAYRLLPSELGPKKRGMRP"
     gene            complement(7513..7809)
                     /locus_tag="VEL68_17430"
     CDS             complement(7513..7809)
                     /locus_tag="VEL68_17430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXZ37808.1"
                     /translation="MISVIAKIPVKEGKMEEAIAAFKELMVQVAKEEGTLSYTINQGK
                     SIPNTLVVMERYRDKAALDAHSSSPHFKAFLPKSAGVMAGRPEITVMEEIHSIR"
     gene            8267..>9312
                     /locus_tag="VEL68_17435"
     CDS             8267..>9312
                     /locus_tag="VEL68_17435"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ37809.1"
                     /translation="MEKCDRLFQYIFNPNSVAIIGASPQDLATLAQMSTKIRDHLFLV
                     NPKYTEVRGMKCYPSILQVEPEIDYAMLIVSATVVPQVLEECIRKGVKVAQIYTAGFS
                     ETGIPERIELENRLKQVAEGRIRVIGPNCFGVYCPRSGLAIVPEGPEEEGHIGVVAQS
                     GSVAESFSYFAKTKNLRFSKVVSYGNAVDLDGCDFLEYLAEDADTKIIAFYIEGTRNG
                     ERLKRALALAAMKKPVVAIKGGMSEQGIRAAVSHTASLTGTPALWRSLFRQAGAIQVE
                     SFDEMLNAVMALDRSPLPSGRGLSIITNSGGFSVIQTDLCQKAGMEVPRFSAETLEAL
                     RSRVPLAGTSIGNPL"
BASE COUNT         2176 a         2409 c         2670 g         2057 t
ORIGIN      
        1 taataataca aatatcgaaa tgaaaatatt tttcaccttc gtagctcctc tttcaattag
       61 ggatagttgc ttatttttaa aaaattgaca attggaaatc ggcaatcgaa aatttcttta
      121 atccgcaatc cgaaatctgc aatctaaaat ttggtgtggg ggcatagctc agctgggaga
      181 gcacaacgtt cgcaacgttg gggtcgaggg ttcgaatccc tttgcctcca ccaataattg
      241 tttttccgcc acggaaaatc cttcccttcc atcctcccaa actttcaccc tcttatcccc
      301 gaaccgcgat gaaaaaagga ttggcagtgt aattccccga gacaaaaaga ggaggattcc
      361 gttataagaa tgccagaaag caaaaccgga gcttcatccg ccccccgaga ggcttacctt
      421 ttatacccta tcataaaaaa accggtgctg cttattatta tggtacatgc tccatagccc
      481 gtgttccatg gcccgtgctt tttcactttt cccaactcac tggcaaggta gttttctgtc
      541 tcgaaaaaaa aataagtaaa ttcccttgac aaaatacagt atttccccca tatcattcta
      601 tgcccggtct catccatgct aagatttagc gagaagaaag cgtgataaaa tgaagaaaaa
      661 gaaattttca gagctggtta ttgtcatcct gatcatactt ttcttcgcta ttccgctctg
      721 cagcctcgtc atgaatgagt cggtggccca ttggcccatt tattcgactc gttttctcta
      781 caatttcctg ttcaaatagc atccgaccgg ccacgtttct ttcttattcg gtgattaatc
      841 atatccgtcc cccaagttct catccactaa catatggtct gaaatccctt ctgcgaaaaa
      901 tctttccccg cccttatcct ttcccccttg tttccccagt gcaccgatat ctctcaagat
      961 tttctgggtc ggagaccagc tccttttccc ttccacccgg atcggtaaag aatctccgcc
     1021 ggatgggtca ggagaagggg gaggcacctt aaggggaatg ggggtacgtc acggttcaca
     1081 gggtgcatgt tctcagcatt ctttctgaat tggctttaca aaagcgaatg aattggataa
     1141 atgtgaatta gccccgggcc ttcgggaacc gatccccctg gaccttgcca tcttggaatc
     1201 cgtggaggga cttagggaaa gggggaagaa acatatttcg aagcaacctg ctcaattctg
     1261 caggatcgag gaaaggagac tctcatgaaa ggagttgtat ttctaggcga gcggcacctg
     1321 gaactaaggg atttccccga acccgtaccg ggttcctcgg aagtcgtcat tgaaatgcgc
     1381 gcttccgggc tctgcggaag cgacttccga gctttccggg cgccccggag cgagcgcgga
     1441 gacccggcgc aactcaaggc tgtggggcat gagccctgcg gccaggtaat caaggtgggc
     1501 ccaggggtgc aaaccctcaa ggtcggcgac cgggtaatca tccatcacta tcttgggtgc
     1561 ggacgttgtc gatggtgcca gatagggtat tcccagatgt gcatagaccc aaaggcgaag
     1621 aaactctact acggcagaac gaatcacgga gggcatgccg agcgaattgc tgtccacgag
     1681 tccgcctgcg tgccgatgcc cgagggcctc agctatgaag aaggagcggc ttgcgcgtgc
     1741 gggacgggca cagcctacga tgccttgaag aggctggacc tttccggaag ggataccttc
     1801 gccatttatg gccaggggcc tgtgggtctc agcgccaccc ttttcggcgt ggcgacaggc
     1861 gcccgagtca ttgtggtgga gccggtcgct tatcggcggg agctggcgaa gagtttggga
     1921 tgtgatgtgg ccatcgatcc ccgcgaagga gacccggttg aggccatcaa gggattgacc
     1981 cacggggagg gggcggatgc tactctcgat tgcaccggaa tccccgaacc ccgcgtaaat
     2041 accgtaaaga gcgccaggat ctatggccga gtctgcttcg tgggagaggg aggcgagacc
     2101 tcattcgacg tcagccggga catcatgcat aagcagctca ccatacacgg ttcctggacc
     2161 atgagcacga tcggcttggg agaagtggca agatacgtgg ccgaacgcaa gctccccttg
     2221 aagaaactga tcacccatcg tttccgtctc gagcaagcgg ccgaggccta caaggtcttt
     2281 gaggcagggc agacggggaa agtggtattt acctgggtct aagaccccgg tcccggaaca
     2341 agttcacgag ggaaagagaa tagccagaga caaggcgggt tcttctttaa acgggttgat
     2401 attaggtcca cgagtcctga ttggtttcaa atccttttcg tctgctctta ccaacaacct
     2461 attcctgcaa agggacgcag gaaaaaacgc gggccagaac ggcatagcag gggggccttg
     2521 gcgttccttt ttccttttgc tcctttcgtt cttttttggt aaactaggct gaattcaggt
     2581 caactagaat ggaggtcaaa aaatgaagac gctctctgtg tccgaggcca agatgaaatt
     2641 aagcggactg attgatacgg tcaaagccac tgacgaggag gttatgatca ccaagaatgg
     2701 ccgcccggca gcagttttgg tgagcccaga ggagtttgag agtctgaaag agacggttgc
     2761 cgtccgttcc gattccagcc tgatgaaaga aatcaaaaag gggcttcggg ccctcaaagc
     2821 gaagaaagcc aaggtgttca ctctcgaaga cctttttgag taaatatttc cggcgggggg
     2881 ccgtcgatga ataaactggt tgtccctaga gagattcatg acctgatccg gaaaatgcac
     2941 cccgatttga agaagaagat tcgggcctgc ttgaaaatga tcctatctga gccaaatgcg
     3001 ggcaaggtgc tgatggatga actttccgga ttgagaagtt tgcgggtgag ttccttccga
     3061 atcatataca agattaaaaa cccaaaggaa attgagctgg tggccatcgg accgagggag
     3121 cgtatatatg aggagacttt ccgcctcatc aaagaatcgg gaggagagag agaagaatag
     3181 cggtgcttgc ggcaaaggaa atccgggatg ttcatgcaga ttgcgctttg gagagaaaac
     3241 gtctggtttt gggttttaga aggaacgttt attgaaaggg ccccggggac atggtaagat
     3301 acgcctgtca ttcccaggaa aggaaaggtt atggtgaaga gcatttcgat cgaatactgc
     3361 aatgattgaa actactaccc ctcagctgcc agtttggcag aggcgatcaa gaaaaaatca
     3421 ggggtagagg cgaagttgat caagtccggg ggtggcgttt ttgaagtcgt ggccgacggg
     3481 aaactgattt tttccaagaa aaaggaacac cgattcccgg agcatcagga gatactggag
     3541 aaaataaagt aatcaaacca gccggagagg atttcgcgcg ggagggggaa aatgaagatc
     3601 accgacgtgg aaagcttcat cgtggacgcg ggctggcgac cctgggtctt cgtcaaagtc
     3661 gaaacggata aggggattgc cggctacggt gagtgcagtg tttccaggtc tccttttgcc
     3721 gtggccggtg ccatagagga cttgaaaccc ctgctcctgg gcacggaccc gagagcctac
     3781 gaaatgaggt tctgggagat gtcacgtcag gcgattgccg agcccctggg gatccgcgcc
     3841 aaggccattg ccggcgtcga actggcgctc ctggacatta aggccagggc tctgggaata
     3901 tcggtggtgg agttgttcgg gggacctacg cgggacaaag taagattgta ctggtctcac
     3961 tgcgggacga cgagggcgct ttacccggag atgggaactc ctcccatccg aaccatggcc
     4021 gatatctcgg cgctggggaa agaggtcgtc cgccggggat acaccgccct caagaccaac
     4081 atcgtctttc cgggggaccc cgcccgcgtg catttcgacg ggtttggaag agggcagggc
     4141 cgggcggttt cgacggatca ggtggcctcc aacagcatgc tgaagcatat tgaaacccta
     4201 atcggcacct tccgggacgc cgtagggccg gaggtggaca tctgcctcga cctgaacttc
     4261 aacttcaagc ccgagagctg catgcggatc gccaaaattc tggagcccta caacctgctc
     4321 tggctggaaa tcgacatgta caacccggaa gccctccggc agatcaagga ctccacgaac
     4381 acgatgatat gcaccggcga gaaccaaata tacatgcagg actatcttcc ctatttccag
     4441 gcccggtgtg ccgatgtgtt catggtggat gtggcctgga tgggcttcag ccaggcaaag
     4501 aaagtcagcg atctcgccct ggtctttgag ttcaacgtcg cgccccacaa ctattacagc
     4561 cacctatcca cgtacatcag cgcgagccta tgcgcggtgc ttcctaacgt ccggatcatg
     4621 gagatcgacg tggacgacgt ccccttgagg gaagcgctcg tcagccgcca gcccgagatt
     4681 caggatggct atatgaaaat tcctacgggg ccgggctggg gaaccgagct taaagaagat
     4741 gtggcccgcc aaaatccctg gcacaagccc aaagcccgct ggtaggtggg ggaagcaatt
     4801 tcggagaccc ggcacggaat ccggacggca gggtcatcct ctcccgggat taaaccggaa
     4861 cccaaacaaa ctgcttataa ctctgcagaa tttttgagat ccgctcctga ggccaggaag
     4921 ggtcccaacc taaccccagg tcctggtcaa ccaggatgac attcaggttg gtttgggttg
     4981 gcttgccttc gatgatggta aagatgttgc atcccctatc cgctgctctg cagtcggtgc
     5041 attctcccgt ggcaacacag ggcgtctctc tctttctgcc ccctagttcg ccggaccgaa
     5101 ttcggaaatg ggtaggagcg atgatgttcc ggatcctctg aaaggcttcg tccaggttgc
     5161 ggacaatctt gtttttcccg acgacaacga tggaaagacg atggccccaa aacatacctg
     5221 ccaccctgtt ccctgttgca tctagattga ccagcctgcc atcctgagtc agagcgttcg
     5281 tccctgtgag gaacccatca cacagggtcc cgtccctgcg gacctggtaa tttcgctttc
     5341 tgaattcctc ggcgctgccc ccctctcctt tgggttcgaa ggggttcaat atcttcgtac
     5401 ccctcttccg cagagccggc agaatgccca gctgccgcat ggaggtggaa tctccaatgg
     5461 ccaaggtggc ctccgcgggg atcagggcca aaatcttctg attggcctcc gcatgatttt
     5521 cggcgaaaaa tccgttgata tgccgggaac ggagtgagga aagggttttt tcaatttcgg
     5581 cttccatcct aagactcctc atttttttga agccattata gcaagaaaat cagaaaagtt
     5641 tctaacttcc aggaagagca atttattgac tttggtctgt cacctccatg tataatgggc
     5701 tggtttttag acctaaaaat gaattccttt tggggaaata cacttcccaa gggaggttgg
     5761 catgggcgat gaaaaggatg gcaaggatca aggttggctg cctgaggtgg aggagcttcg
     5821 acatcgtgag tccctggctt ctcagatggg gggcccagag aacctggcga ggcaccgggc
     5881 tgccggccgg ctgaatgtgc gggagcggat tgacagactc ctggacaaag gaagcttcca
     5941 cgagaccggg gccatcaccg ggatccccac ttttgagggt gaccgtctgg tcaagctctt
     6001 tccttcgaac ttcatcatgg ggacgggaag gatcaataac cgcaaagtgg tggtgggctg
     6061 cgacgacttc accatccggg gaggggccgc tgacgcggcc atcgggagga aggcgcccta
     6121 tgcggagcgc atggcccttg atctgcgcct gcccatgatc cgtttggtgg atggaaccgg
     6181 cgggggagga agcgtgaaat atctggaaaa gatggaccgt acctacatcc cggccaaccc
     6241 ggccatggag attatggaac ctctgctctc taccgtgccg gtcatgagtg cggccctggg
     6301 atcggtcgcc ggcctgggcg cggcccgggt ggttttttcc catttttcgg tcatgcccca
     6361 aaaaacggcc cagctcttcg tggccgggcc cccggtagtg gaagccgctt tcggaaagag
     6421 ggtggagaag gaagagctgg gaggctcccg aatccatacc accgggtttg gcgcggtgga
     6481 caacgaggtg gcttccgaag aagaggcgtt cgaacaaatc cgtcggttcc tctcttatat
     6541 gccgaattcg gtgtacgagc tccctccggt gaaggaggcc aaggaccccc gcgaccgacg
     6601 cgaggaagag cttattggca tcgtaccgaa aaacaaacgt ctcccttatt cggtgcgtcg
     6661 tatcctagaa ctggttcttg accaagggtc ctttttcgag atcggggcga agcatgggcg
     6721 gtcggtggtt accggcctgg cccgcctcct gggtcggccc atcggggtga tggcccacga
     6781 tccgaagttc tacagcggtt cggttaccgc cgagggggcg gacaagatgg ttcgtttcat
     6841 tgacctgtgc gacaccttcc atttgcccgt agtttatttt gtggataacc caggtttcat
     6901 gatcggagtg gaggcggaga aacaggcgac catccgtttc ggagcgcggt acatatttgc
     6961 ggtgtaccag gctacggttc cctggtgctc catcatcctg aggagggtct tcggagtggc
     7021 cggggcagcc catgggaatc actcccgact gaatttgcgc tatgcctggc cttcgggaga
     7081 ttggggatcg ctaccgattg agggaggggt tcaagcggct tataggcgac agatcgaggc
     7141 ggcgcaagac cctcaggctt tgagagaaga gatcgaaaag cgcctgacca aggtgacttc
     7201 gccctttcgc acggccgaga tattcggggt ggaggagatc atcgacccgc gagacacccg
     7261 gccgctgctc tgcgagtgga tcgagaccgc ctaccgactg ctcccgagcg agctgggacc
     7321 caaaaagagg ggaatgcgcc cctagctgtt tgctgccaaa caaatacaaa cgaaataaat
     7381 atttttacgc catcgcgagc ccgaagggaa cggcaatctc attcctgatt cggattgctt
     7441 cgtcgcttcg ctcctcgcaa tgccacgcta cttaaacccg ccattcctcg tttgcccggc
     7501 ccctcagggg ggttagcgga tcgagtggat ttcctccatc accgtgatct cgggccttcc
     7561 ggccatgacc ccggcgctct tgggcaaaaa ggccttgaaa tggggcgagg aggagtgagc
     7621 gtccaaggcg gccttgtccc tataacgctc catgactaca agagtattgg gaatggattt
     7681 gccctggttg atggtgtaac tcagggtgcc ttcttccttg gccacctgga ccatgagctc
     7741 cttgaaggcc gcgatggctt cctccatttt tccttctttc accgggatct tggcaataac
     7801 tgagatcatc ttagaccctc cattggttga atgatgcctt tcggataaaa ccattaacaa
     7861 ccagcctata attgtataga cgctgaagaa taagccgttc gtgccgagct tgtcgaagca
     7921 tgaacggcgt agcgacgcac ccttcgacaa gcatgtcccg agtgagccgt tcgcccttcg
     7981 acaagctcag ggcgaacggt gaatcaaagg gctcagagta aacggtgcca gccgaagaga
     8041 ggagcatagg attagaaggg atattcggaa gaatatgtct tttcaattat ggactcctta
     8101 gtaacacata gcccattttt agtcaacttt ttccaaccgc cacaacaagt gggtggatcg
     8161 atcgaaagaa cctgcctgag ggcttgcccg gaaggttttt ttattctatt gttgatccgt
     8221 tgtggcaata aagaaggcag gatcagggca gacggaggaa aactgaatgg agaaatgtga
     8281 ccggttgttt caatatatct tcaaccccaa ttccgtggcg atcatcgggg cgtcgcctca
     8341 ggacctggcc accctcgccc agatgagcac gaaaatcagg gatcatttgt tcctggtgaa
     8401 cccgaaatac acggaagttc gcggcatgaa atgctacccc agcattttgc aggtggagcc
     8461 cgagattgac tacgccatgc tcatcgtgtc ggccaccgtc gtgcctcagg ttttagaaga
     8521 atgcatccgg aaaggagtca aggtcgcgca gatttatacc gccgggttca gtgagaccgg
     8581 gattccggag aggatcgaac tggagaaccg cctgaagcaa gtggcggagg gcaggatcag
     8641 ggtcatcggc ccgaattgct tcggggtcta ttgtccccgg tccgggctgg cgattgttcc
     8701 ggagggaccg gaggaagagg ggcacatcgg agtcgtggcc cagagcggaa gcgtggccga
     8761 atccttctcc tatttcgcca agaccaagaa cctgcgattc agcaaagtgg tgagttacgg
     8821 caatgccgtg gacctggacg gttgtgactt cctggaatac ctggccgaag atgcggacac
     8881 aaaaatcata gccttctata tcgaaggcac ccggaatgga gaacggctga aaagggcgtt
     8941 ggccttagcg gcgatgaaaa agccggtcgt cgccattaag ggaggaatga gcgagcaggg
     9001 gatcagggcg gccgtttccc ataccgcttc cctcaccgga accccggcgc tatggaggtc
     9061 tctcttccgg caggcggggg ccattcaggt ggagagcttc gatgagatgc tgaatgcggt
     9121 catggctctg gaccggagtc ccctgccttc gggaaggggc ctttccatca tcaccaactc
     9181 cgggggtttc agcgtgatcc agacggacct gtgccagaag gcggggatgg aggttccccg
     9241 cttttccgcc gaaaccctgg aggctctccg gtcccgggtt ccccttgccg ggaccagcat
     9301 cggcaatccc ct
//
LOCUS       DASPRR010000474         7271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1241725, whole genome shotgun sequence.
ACCESSION   DASPRR010000474 DASPRR010000000
VERSION     DASPRR010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241725"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VEL68_17440"
     CDS             complement(<1..1266)
                     /locus_tag="VEL68_17440"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXZ37810.1"
                     /translation="MEIVESDVLIIGSGGAALRSAIEAKVTFPGGRITILTKGEMGKC
                     GVTAISCSDRMAFHATLPYTAPGGPDNWKHHARDIFQIGGFVSDADLAAILARESGEA
                     FEFLDGLGVPFAKTAEGKADQFITDGSEYPRACYTGPRTANHIEEALVRRVSSMDIQV
                     VEHCMACDLLLEEGRVIGAIGVDTQKGSKPLPGRLKIFLSKAVILATGGAGEMFAVHV
                     YPPGMTGDGYAMAYRAGAELVNMEFIQIGPASVKTQLNCSGSFMRANPRFVNNQGEEF
                     LPRYFLPDTPLSVIHNLCFEKGSTWPVSREKKTHRIDVGVSKETAQGRKVFLDYSRNP
                     SGFRFQDLEARWQERYRRETKEDGKEEERNQSPLIRLKEINPESIEWLKENGINLEGG
                     ELLEIAEAGQHFQGGVKIRAKGNATLQSLY"
     gene            complement(1403..2692)
                     /locus_tag="VEL68_17445"
     CDS             complement(1403..2692)
                     /locus_tag="VEL68_17445"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ37811.1"
                     /translation="MGTIFILLASFGVLLVINVPVAFSMTLACILALFWQGTIPISTV
                     TLKLYSGIDTFPFLAIPLFILAGGLMEQGGISQRLVRFAKDLVGHIKGGLGFVVVVSE
                     IFFSGISGSSIADASAIGSLLLPSMVRAGYEAPRAAAIVAAATGMGMMIPPCLNMVVL
                     GAMANISIAGLFMGGFLPGFLMALTLMVIIYIQSSRGILPGAEGMRASFGEILVAFKD
                     SIIPLMMPVIIFGGIFTGIFTATEAASVATVYAFIVSFFIYKEIKLNDLKKILVDTAV
                     MTGVVCLLVGAATMFSWILATHQVPRAIGDFIGSIGGGYTVFLLMTVAVFLFFGMILD
                     GLPAILIFFPILYPIAQSLGIHPLHYGVLVIAIVGISIVAPPIGLCLVIICSLAKIKL
                     TDTIKPLIPYTLILVGDLVVIAFWPWLILFLPTLFKL"
     gene            complement(2696..3169)
                     /locus_tag="VEL68_17450"
     CDS             complement(2696..3169)
                     /locus_tag="VEL68_17450"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ37812.1"
                     /translation="MLKRILANLVEGVCMVLVVALAVVVFLQVFNRFVLKAPLAWSED
                     LAMLLFQWVAFLGAAVGVKRVRHFGIELVVKRMSAKARHWIEILIPWIMGCVGLTMVT
                     EGYNLLLFNKNRIYSSMDLSYVWTYSAIPVSGVLIIIFLIQQEVGRLRGCKEGGK"
     gene            complement(3245..4246)
                     /locus_tag="VEL68_17455"
     CDS             complement(3245..4246)
                     /locus_tag="VEL68_17455"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ37813.1"
                     /translation="MKKIAMMLLIGVFALAALSGMAQAQEKKYVAKLADILSPDHPHT
                     RSWVYFADKVKEKTKGQVEIQVFHSGQLGQTKDLYLGMQMGNVEMAKLPVSFTGEWIP
                     EVYVFDLPYLFNNREEMWKVFKSPVGAKFFDEIYAKQNLVGMMWADDGGRSIYTVNKP
                     IRKPEDLKGLKIRVQPSAIHIQSINEMGGIATPTAWGEVYLALQQKVLDGAENSPVLF
                     WTSKHWEVCKAYSMTQQFWLPTPLLASKKWWDSIPKDLQAQVRAAAAEAEKYFVQIYT
                     ADEDKALGWAKEKGVQVITDVDKAAFVKRIQPVYETFVKKYAFGKELLSQVQAVNKK"
     gene            complement(4352..5548)
                     /locus_tag="VEL68_17460"
     CDS             complement(4352..5548)
                     /locus_tag="VEL68_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ37814.1"
                     /translation="MAPLRIGMAGAQFAARLHLENYFPLRGSKVEITAIASRTKGRAE
                     ELARKYNIANLADDYRALLDRKDVDAVDLCVPTDLHHEFIIQAAQAGKHVICEKPLTG
                     YFGKDRPEDLVGQTVDRKTMLREALANCEKVAQAIRKNKVQFCYAENWVYAPPVAKLK
                     KLMQVSRGTVLDVRAEESHSGSHAGYSRKWKTSGGGALLRLGAHPVSTVLHLKQYEGS
                     LREGKGIRLKSVTAEVGQHTRIESFKKEPQKWIVDKWEDVEDWATMVMNFEDGSQAVV
                     SASDGVLGGVRNTMTVYLSNAVVQVNINPSDTVFAYAPAPHIFGDEYISEKLETKAGW
                     NFASPDEDWVRGYPQELADFADCLLSGREPVSGLDLALETVKAIYAAYLSAEQGNRVE
                     LNKNII"
     gene            5842..7044
                     /locus_tag="VEL68_17465"
     CDS             5842..7044
                     /locus_tag="VEL68_17465"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HXZ37815.1"
                     /translation="MNLKAEKDYFDILLQDGEIIDPAQGIHLTGSVAIKNGLIAAVGK
                     DVAGKAEKVIPLQGKLITPGLIDVHCHPSLGFRTRGIPPDEVGLNSGVTLLGDGGTSG
                     PANFFAFRKLVVEPARTEILCFLNVAKAGLISQPEIYSLRDMDVDRGKEVIEANRDLI
                     RGVKIRVIQALAEGVGIKGVEMAKKLADDLHLPLMIHIGDPRERMAKDPMDDFSRQAV
                     SLLEKGDILSHYLTWEPGGLILRDGTIYPELEKARKRGVVLDSCHGLWHFSFTVARHA
                     LTRGFKPDVISTDMSIPNLLVTQSLAVTLSKFLCLGLSLEEVIAMTTINPARFLGEES
                     KRGSLKVGLSAHITVSELIKGDFLFGDGKGRETLHGNLLLEPRMVFKSGEMMPSYSRY
                     HIPPLFKD"
BASE COUNT         1619 a         2097 c         1941 g         1614 t
ORIGIN      
        1 atacaggctc tggagtgtgg cattcccctt cgcccgaatc tttactcctc cctggaagtg
       61 ctggcctgcc tcagcgattt ccaggagctc tcccccttcc aggttgatcc cgttctcctt
      121 caaccactcg atggactcgg ggttgatctc cttcagtcgg attaaaggag attgatttcg
      181 ctcttcctct ttcccatctt ccttggtctc ccgcctatat ctttcctgcc atctcgcttc
      241 caggtcctgg aagcggaagc ctgaaggatt tcgactgtaa tccaggaaca cttttcttcc
      301 ctgggctgtt tctttggaaa cgccaacatc gatccgatgg gtctttttct cccgggagac
      361 cggccaggtg gaaccctttt cgaagcatag attatggatg accgaaaggg gagtgtcggg
      421 gagaaagtat cggggcaaaa actcctctcc ctggttattg acaaacctcg gattcgctcg
      481 catgaagctc cccgagcagt tgagctgggt tttgactgaa gctgggccga tctggatgaa
      541 ttccatgttc accagctcgg cccccgcccg gtaagccatg gcatatccgt cgccggtcat
      601 tcccggggga tagacatgga cggcgaacat ctctcctgcc cccccggtag ccaggattac
      661 ggctttggat aaaaaaatct tcagcctccc gggcaagggc tttgatccct tctgggtatc
      721 cacccctatg gccccgatca cccttccctc ttccaggagc aaatcgcagg ccatgcagtg
      781 ctccacgacc tggatatcca tggaagagac ccgtcggacc aacgcctcct caatgtggtt
      841 ggcggtccgg ggaccggtgt agcaggcccg gggatactcc gatccgtcgg tgatgaactg
      901 atccgctttc ccttcggcgg tcttcgcaaa gggaacgccc agcccgtcca ggaattcaaa
      961 tgcctctcct gattctctgg ccaggatggc cgccaggtcg gcgtccgaga caaaccctcc
     1021 gatttgaaaa atgtcccggg cgtggtgctt ccagttgtcc ggtccgcctg gtgccgtgta
     1081 aggcagggtg gcatgaaagg ccatccggtc cgaacaggaa atagccgtga ccccacactt
     1141 acccatctcc cctttggtca ggatggtaat tcgccctccg ggaaaggtta ccttggcctc
     1201 gatggccgac cgcaaagcag ccccaccgct gccgatgatc aggacatcac tttcgacgat
     1261 ttccatgaat actccagcag gaagacccct tatctcaaaa aaagaaaacc tctatttctt
     1321 gatctcacaa cctcccggct catttcggcg cctcctcccc ctcacccaac cctctcccgc
     1381 tgggggaaag ggtaaaaatg gattaaagtt tgaagagggt gggaaggaat aaaatcagcc
     1441 acggccagaa agcgatgacc actaagtctc cgacaagaat aagcgtgtaa ggaatcaagg
     1501 gcttgatggt gtcagtcagc ttgatcttgg ccaggctgca gatgataacc aggcagaggc
     1561 caatgggcgg agccacgatc gatatgccga cgatggcaat gaccagaacg ccgtaatgga
     1621 gagggtggat cccgagagac tgggcgatgg gataaaggat ggggaaaaaa atcagaatcg
     1681 ccggcaggcc gtccaggatc atcccgaaga aaaggaagac cgctacggtc atgagaagga
     1741 agactgtgta tcctcccccg atggacccga tgaagtctcc gatcgccctg ggcacctggt
     1801 gggtggccag aatccaggaa aacatggtgg ccgcccccac caggaggcaa accacgccgg
     1861 tcatcaccgc tgtatccacc aggatctttt tcaggtcatt caacttgatc tctttataga
     1921 tgaagaacga gacgatgaag gcatagactg tagccacgga ggccgcctcg gtggccgtga
     1981 agattccggt gaagatcccc ccgaagatga tgaccggcat catcaggggt atgatgctgt
     2041 ctttgaaggc caccaggatt tccccgaagg aggccctcat cccttcagcg cccgggagaa
     2101 ttccccggga ggattgaatg tagatgatca ccatcaaagt cagggccatg aggaatccgg
     2161 ggagaaagcc gcccatgaaa agcccggcaa tggagatgtt agccattgcc ccgagaacca
     2221 ccatgttcag gcagggcggg atcatcatcc ccattccggt cgccgccgct acgatggccg
     2281 cagccctcgg ggcctcgtac ccggcccgca ccatggaagg caggaggagg gagccgatgg
     2341 ccgaagcgtc ggctatggaa gacccggaaa ttccggagaa gaagatctcc gagaccacca
     2401 ccacaaatcc cagcccgccc ttgatatggc cgaccaagtc tttggcgaag cggaccagtc
     2461 gttgggaaat ccccccctgc tccatcaaac ccccggccag gatgaaaaga ggtatggcca
     2521 aaaacggaaa ggtatcaatg ccggaataaa gctttaaggt gaccgtggaa atgggaatgg
     2581 ttccctgcca gaaaagggcc aggatgcacg ccagggtcat ggagaaggcc acggggacat
     2641 tgatgaccag caggacgccg aaggaggcca ggagaatgaa gatggtgccc atcgcctatt
     2701 tcccaccctc cttacagccc cgcaaccgcc caacttcctg ctggatcagg aagatgatga
     2761 tcaaaacccc gctcacgggg atcgccgaat aagtccagac gtaggataag tccatggagg
     2821 aatagatgcg gttcttgttg aacaagagca gattgtaacc ctcggtgacc atggtcagac
     2881 ctacgcatcc catgatccag gggatgagga tttcgatcca gtgccgcgcc ttggcggaca
     2941 ttctcttgac caccaactcg atgccgaaat gacggacccg cttcactccg accgcggctc
     3001 ccagaaaggc cacccattga aagaggagca tggccaggtc ctcggaccag gccagcgggg
     3061 ctttcagaac gaaccggttg aagacctgaa gaaagactac cacggccagg gccacaacca
     3121 gaaccatgca aaccccttcc accagatttg ccagaatcct tttcaacatc cactcccttg
     3181 ccaaatgatg ggaaaagggg aagccatggt ctcccggaac atggctcccc ttagagccgg
     3241 cttcctattt cttgttgacc gcctgaacct ggctcaacag ctctttgcca aaggcatact
     3301 tctttacaaa ggtttcgtag accggctgga tgcgcttcac aaaggccgct ttgtccacgt
     3361 cggtaatcac ctggactcct ttttccttcg cccatcccag ggccttgtct tcatccgctg
     3421 tgtaaatttg gacgaaatat ttttccgctt ccgccgcggc cgcccggacc tgggcctgaa
     3481 ggtccttggg gatcgaatcc caccacttct tgctcgccag aagaggagtg ggcagccaga
     3541 actgctgggt catggagtag gctttgcaga cttcccagtg tttggaggtc cagaagagga
     3601 cgggcgagtt ttcggcgccg tccagcacct tctgctgtag ggccaggtag acttcgcccc
     3661 atgccgtggg tgtggcgatt ccgcccatct cattgatgga ttggatgtga atggcgctgg
     3721 gctgtacccg gatcttcagc cccttcaggt cttcaggctt gcggatgggc ttattgaccg
     3781 tatagatgct ccggcccccg tcatccgccc acatcattcc caccagattc tgcttggcat
     3841 agatttcgtc gaaaaacttg gcccccaccg gacttttgaa tactttccac atctcttccc
     3901 ggttgttgaa gaggtagggc aggtcgaata catagacctc gggaatccat tctccggtga
     3961 aggaaacggg caacttggcc atttctacgt tacccatctg catacccagg tagaggtcct
     4021 tggtctgtcc cagctggccg ctgtggaaaa cctggatttc aacctggccc ttggtcttct
     4081 ccttcacctt gtcggcaaaa tacacccagg accgggtgtg gggatgatct ggagagagga
     4141 tatccgccag tttggctaca tacttttttt cttgcgcctg ggccatgccg gatagggcgg
     4201 ccagggcaaa aactccgatc aaaagcatca tggcgatttt cttcattgaa ttcctccttg
     4261 gttttatttt cgatattttc gctctatagt gaaaacgccc atttttatca aaacgcacga
     4321 tcgaattctc ctctctacct cctcctttct ctcaaatgat attcttattc aactctaccc
     4381 ggtttccctg ttccgccgaa aggtaggcgg cataaattgc cttcacggtc tccagggcca
     4441 ggtcgaggcc ggaaaccggc tctcggccgg agagcaggca gtccgcgaaa tccgccagtt
     4501 cctgggggta gcctctcacc cagtcctcgt ccgggctggc gaagttccag ccagccttgg
     4561 tctccagctt ttcggaaata tattcgtcgc cgaaaatgtg gggggcagga gcgtatgcga
     4621 aaaccgtatc gctgggattg atgttcacct gcaccacggc gttggacaga tagacggtca
     4681 tggtgtttcg caccccgccg aggactccgt ctgaagcgct gactacggcc tgggaaccgt
     4741 cttcgaaatt catgaccatg gtggcccagt cttccacgtc ttcccattta tcgacaatcc
     4801 acttctgcgg ctcttttttg aaagattcga tccgagtatg ctgtcccacc tcggcggtta
     4861 cggatttgag ccgaatcccc tttccttctc tcaggctccc ctcatactgt ttcaggtgga
     4921 gcacagtgga gaccggatgg gcgcccaagc gcagaagggc tccccctccc gaagtcttcc
     4981 acttgcggga gtaacctgca tgggagccgg aatggctctc ttcggcgcgc acgtccaaga
     5041 ccgtaccccg gctgacctgc atcagctttt tcagcttggc caccggcggg gcatagaccc
     5101 agttttcggc gtagcagaac tggaccttat tcttccggat ggcctgggcc actttttcgc
     5161 aattcgccag agcctcccgg agcatggtct tccggtccac ggtttgtccc acaaggtctt
     5221 ccggccggtc tttcccgaaa tagcccgtaa gaggtttttc gcagatgaca tgtttcccag
     5281 cctgggccgc ctggataata aattcgtgat gcaaatccgt gggcacacaa aggtccaccg
     5341 catccacatc tttgcggtcc agaagggccc ggtaatcgtc ggccaaattg gcaatattgt
     5401 attttcgggc cagctcttcc gccctccctt tcgtccggga ggcgatggcc gtaatttcta
     5461 ctttcgaacc gcgcaaggga aagtaattct ccaggtggag gcgcgccgca aactgcgctc
     5521 ccgccatccc gattcggagc ggagccatga ggttctcctt tttcagcgaa tagagccttt
     5581 cttgaaggga cagcctttct tcgcagctaa gagagggatt ccctttaaaa aatataatac
     5641 ggttccattt tattcaaccg cttttttggg ccgccccaag gctgtccacc ccctagggaa
     5701 gcaagaacgt ttgcttaagg actttccagc gggcagggga tttggtagaa tgcccgcggc
     5761 ttctcgtccg gataatcctc ctggcgattt cgctttttag aaggcatctc cgcctagggt
     5821 ctcaaaggag agggaacgga tatgaatctt aaggccgaaa aagattattt tgatatcctg
     5881 cttcaggacg gagaaatcat cgatccggcc caaggaattc atttaaccgg ttccgtggcc
     5941 attaagaacg ggctgattgc cgccgtggga aaggatgttg ccgggaaggc cgagaaagtg
     6001 atccccctgc aggggaaact gatcaccccc gggttaatcg atgtgcactg ccatccttcc
     6061 ttgggattca ggacacgcgg aattcctccc gacgaagtgg gtctgaattc cggggtcact
     6121 ctcctgggcg atggcgggac ctctggccca gccaatttct ttgccttccg caagctggtc
     6181 gtggagcccg cccggacgga aatcctctgc ttcctgaatg tggccaaggc gggtctcatc
     6241 agccagccgg aaatctactc cctgcgggac atggacgtgg accgcgggaa agaagtgatc
     6301 gaggccaacc gggacctgat ccggggagtc aagatccggg tcattcaagc tcttgccgag
     6361 ggagttggca tcaaaggtgt ggagatggcc aagaagctgg ccgatgacct tcacctgcct
     6421 ctcatgattc acatcggaga cccccgggaa cggatggcaa aagacccgat ggacgatttc
     6481 tcccgccagg ccgtttctct cttggagaag ggcgacattc tcagccatta cctaacctgg
     6541 gagccgggag ggctcattct gagagacggg accatctatc ccgaattgga gaaagcgcgg
     6601 aagaggggag tggttctgga ttcctgccat ggcctttggc acttcagctt caccgtcgcc
     6661 cgccacgccc tgacccgggg attcaagccc gacgtgatca gcaccgacat gtccatcccc
     6721 aaccttctgg tgacccagtc cctggccgtg accctgtcaa agtttctctg cctggggctc
     6781 agcctggaag aagtgatcgc catgaccacc atcaacccgg ccaggttcct cggggaagaa
     6841 agcaaacggg gaagcttgaa ggtgggcttg tctgcacaca tcaccgtttc ggagctcatc
     6901 aaaggagact tcttattcgg agacgggaag gggcgagaaa ccctgcacgg gaacttattg
     6961 ctcgagcccc gaatggtttt caagtccggg gagatgatgc cctcctattc ccggtatcat
     7021 atcccgcccc tcttcaaaga ctaactcggt gtcagtttca gtgtcatgtt catttcagca
     7081 gggtcttaaa ggccatccat gggaattaag aatttccagg gaaccctatc caagaatttc
     7141 tttgtatttt caatgattaa atcctccttt tctgcattct atgctctacc tgaatcccga
     7201 taattaaaga aaattcttcc gccaagattg ggagcagatc ggcaggctgg tgcaaaaact
     7261 ggggttgcaa a
//
LOCUS       DASPRR010000475        14789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_600396, whole genome shotgun sequence.
ACCESSION   DASPRR010000475 DASPRR010000000
VERSION     DASPRR010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14789)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14789
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600396"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1430
                     /locus_tag="VEL68_17470"
     CDS             246..1430
                     /locus_tag="VEL68_17470"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ37816.1"
                     /translation="MGSEEITRRKFIENVAKYCGAGIAAFGLMGPGKAHGVADLDSPA
                     HDGVWRMRDSAVMQSGEWRKNAPIPPDYFDRKPEHFINDGKAVEWSHLHENWYAEGDK
                     SLVGLIMATQLDKDLKGDIRKAVRLVGGFQKSLKKTDKILIKPNMNSVHEWPGGGTDA
                     AFLEALIFNLKDEGFENLTVADTCGPWGPTARVAKSLGIEAVCNSTKTTLRDWEKTKW
                     MNIRNAKAEFLGKFGGGDGTVAYPEDLRNYDKIIYTPVMKTHYLGGITMSLKLSVGML
                     YRPDRPAQLHTFNHLFVPQAAAEINLPLRPDLIIMDGRRSFISGGPSHGELVKPGMIV
                     VSGDQVACDVTGINILQEWYPNLAQNKITTYPWYQKQVRQAVKIGVGYAKKEEDVKLV
                     LG"
     gene            1566..1946
                     /locus_tag="VEL68_17475"
     CDS             1566..1946
                     /locus_tag="VEL68_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37817.1"
                     /translation="MMKTATLFAILIFTVFLIVPGESPSQSSTFAEFKVDALYRAAKV
                     SWKTAGDPKAEMSVQIMRADTFEEGPYQEVGVVKITPGKSSYEYIDKSMGAEAKYYYK
                     LIIKETNESMGPKPTRPFFSPPAT"
     gene            2053..2658
                     /locus_tag="VEL68_17480"
     CDS             2053..2658
                     /locus_tag="VEL68_17480"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ37818.1"
                     /translation="MPQEVNRRTVLKGITLGAFGLGSVVAVGNLLEVLTFPNGQRVAL
                     AKGVILVDKSLCSGCRVCEIVCANYNSGGRNSPSLSRIILEKEIMGGDYQPKVCHQCV
                     DPPCLKACPVTALQVDKQRGTYARVIDERVCIGCQKCVEACLQHFRPARPRYDAQRHR
                     SVKCHLCFGDPQCVKFCPLGVLRLERSEKGLMVGYPVIQED"
     gene            2813..4846
                     /locus_tag="VEL68_17485"
     CDS             2813..4846
                     /locus_tag="VEL68_17485"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ37819.1"
                     /translation="MNPKGGWVGKILRVDLTSGKTSEQSTYDYVPKFMGGRGLGAKVC
                     WDEVPPEVGAFDPENRLILATGPLQGTLAPTSGRFMVLGKAPQTGPIESFCRSGVGGH
                     FAPELKWAGYDAVIIQGKAPKPVYLWITDHKAEILDATRLWGLDTYQTQQMIWKLHGP
                     KTRVMVIGRAGETKSRIACILTDTGDASGQGGYGGVMGSKNLKAIAVRGTGTVNVARP
                     KDLMEITRHIHSLFSRKSLKSDPYQPEEKNFKYNIWGGGYGRGSLSLLPGELLDLCNS
                     PSSGYSQTPDGCFACPVSCRSRVKGPDITPGVAFCAQSYMYLESMVHQPEKGYSKVTW
                     QAAKLGDLYGINAYELMAIIPWLSDCFREKLIKEEEVGLPLKEIGSWDFISKLLYKMA
                     NREGIGDLLAEGGQRAAAKLGGKAEKLSHMYYPRAGKFAGYREHWVYLGGFPTGYAVP
                     QLALMWVLDNRDAMVSHSYISALWGAAFTVGQNALTAVPEKIIPVLKPVMKAAYGSAE
                     AAEFVRPDGKGLNWKWAAPVVKRYHEHSLLKDSYILCDILFPFLFDANTPDHVGDLTL
                     ESRIFSAVTGRELNLEESYKMGEMLSTLERSLAARDGRARKDDILHDIYFEKEDAGGR
                     KYKREDVERAKSEYYQLMGWDAKAGVPTAGTLEKLGLKEVAEGLEKRGLLGKK"
     gene            5032..5853
                     /locus_tag="VEL68_17490"
     CDS             5032..5853
                     /locus_tag="VEL68_17490"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HXZ37820.1"
                     /translation="MSETYSLPAFTMTDYNLYRVDDRIFYVFILAALAFCLIHAARRA
                     FRQPKPTEVPSLKSTSGNEEQMERHTLFQRIFHWANAIAIFILAGSGWMIYQPGRIPL
                     MEGPPSEWFLWHRWGVAFLLAGIAAHIIREAFLAQEANPMVFDRAEVRRILSIFKNFF
                     GLSKNYPLAGKYHTGQLIFHWAVAGNLFLLILTGMVLWKPLRGFLPLSLFGLGWDFIY
                     FTRILHGFFSATLFASLIGHIYFALFIKKNWPEAKSMVTGRMPLQEYLTSHSLPD"
     gene            6349..8274
                     /locus_tag="VEL68_17495"
     CDS             6349..8274
                     /locus_tag="VEL68_17495"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ37821.1"
                     /translation="MAEKYPHLFSPVTIHTMTLKNRVVMPPMCTDYATIGGAVNDRLI
                     EYYATRARGGVGLIDVEFSYVHPAGKVFEHMLGIYDDKLIPGLRRLTDAIHQGGAKVI
                     VQIAHGGRRGHSDITGATIVGPSSIPRLNGETPKELSPAGIEDLIQAFILAARRAKKA
                     GFDGVMIHMAHAYLLQQFLSPFSNVRTDRYGGDLEGRARFPLEVVKGVRREVGKDYPI
                     TCRLCGDEFLKEGFDLSQSIRVAKMLEANGVNAIEVSAGAHETGYVITAPPYYPMGFL
                     SPLSEAIRKEVNIPVGAVGRIHTPEVAEDLLRQGKADLIAVGRGLIADPDWPKKAQEG
                     RPETIRPCISCNQGCGDRMYLHRDISCTVNPAVGREGTFPVEPARKKKKVLVLGGGPA
                     GLEVALVAARRGHTVHLYEKEKELGGQLNIAAVPPAKEKIREFKEFLIREVKGLPIKV
                     VHAKPDARTLKKISPDLVVVAVGGKPKVMEGLGFDDRKVISAWDALSGKKPVGRRVVI
                     VGGGQVGLETAHFFLEKGKDVTVLEMLKSAGQDMSPRARKLILEKLVQGGAEVLTEAK
                     ALSVEKGDLTFERAGLREKIKGADSIIIAVGTASQDEEIPGLKRSRIPFRRVGDASAP
                     RKLIEAVHEGYQVGVEI"
     gene            8461..8835
                     /locus_tag="VEL68_17500"
     CDS             8461..8835
                     /locus_tag="VEL68_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37822.1"
                     /translation="MNMIEKLPELVNGNERLVWRGRFLTVDFLIEVGDVSYLVSIQAG
                     RIVSVEQGPFYMRPWRFAVRASATAWEKLWEPMPRPGYTDILGLMRRGEARIEGDLQP
                     LVANLRYVKEVLVTPRRLKGGC"
     gene            8837..9694
                     /locus_tag="VEL68_17505"
     CDS             8837..9694
                     /locus_tag="VEL68_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ37823.1"
                     /translation="MAAQIEPIVGRYIQLRLGGRACRIYFEETGKGIPLVCLHTAGSD
                     TRQYRHLMVDPAVTEHFRVLAFDLPWHGKSNPPPGWHEEEYRLTTEAYLEATMGFCGA
                     LELNRPVVMGCSMGGRIVIPLAAEYGKELRAVIGLEAGYQVQPWFDNTWLHHPDVHGG
                     EICAARVSGLMAPQSPEETRWETLWGYMQSGPGVFKGDLYFYFQRAESDFLNYMARID
                     TKACPVYLLAGEYDYSCTPEDTQRTGAYIPGSEVMIMKEMGHFPMSENPVAFRRYILP
                     VLEKIRNQK"
     gene            9839..10819
                     /locus_tag="VEL68_17510"
     CDS             9839..10819
                     /locus_tag="VEL68_17510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37824.1"
                     /translation="MKSSRMISLVVVLLACLAMGTQVRGADNFPAKPLELVVPYVAGG
                     STDVMCRTIVARALPFFNNQPVMVVNKPGGGTVIGSRYVLDNRNDGYTLYSTSTASMM
                     IAPVINKTNFSWRDFIGVAQTMLGSDALYVPGDASYNTLEKFIDYAMQNPGKIKYTTA
                     GTGGSSHLPMEGFAVAKGLSLKHIPTKGDAENIAALMGGHVTAGAGNPIAFQPHVASG
                     KFKCLVQFGAERDKAFLPNIPTFKEFGIDVAVDLWRWVVVPKGVPPERVKFLAEAFKK
                     IFQDKEVLASLEKIQCPASYLPPEDYEKAMKASEASILPLIKASKIMEAK"
     gene            10932..12797
                     /locus_tag="VEL68_17515"
     CDS             10932..12797
                     /locus_tag="VEL68_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ37825.1"
                     /translation="MKGYNGKILRVNLSTRQVSTEAPTEEYYKTYLGGRGFIVHTLLK
                     EVPRGADPLGPENKLIFALGPITGHPFPGSGRNSVGAKSPLSGGFGESEAGGFWGAEL
                     KRSGYDAIIVEGKADKPVYLWIKDGQTEIRDARNLWGLDVAETEKAIQEELQDGRVRT
                     ATIGVAGEKLVRFASICNDISHVAGRTGMGAVMGSKKLKAVAVRGNTPPEMGNREKVL
                     ELSRWMGQNFKTKTNFWQYGTGAMMDYYEASGNLPIRNFAAGRFPEVEDITSQSMYKK
                     GYVEKMESCYGCPVRCKKRVKLEQPWKVDPIYGGPEYETLGAFGSCCGIGQVEAIMKA
                     NELCCRYGMDTISAGVTISFAMECFEKGVLTLKDTDGLELTFGNASAMVEMVERIALR
                     KGLGNLLAEGTKRAAEVIGKGSAEWAMHVKGEEIPMHEPRYKQGMGLHYSVHATGADH
                     CSGPHDDVVNKKLPEWDRIGLAESIPVYEMSGRKARMVYEVGLWRHVANSIGLCLFVP
                     WSNEQVTQAVEAITGWPISFWKLMKTAERSITLARVFNLREGFTHRDDTLPRRFASSP
                     ADSNLKGVGVDPEKLSSAQKVYYQMMGWDENGVPTRARLIELNIEWASQYLPAGR"
     gene            13276..14535
                     /locus_tag="VEL68_17520"
     CDS             13276..14535
                     /locus_tag="VEL68_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37826.1"
                     /translation="MKRFFFLSFVLILTILPLQAMAQDNVKIGCVLPYTGALAWQGQE
                     TFRGTQIAADMQNKRGGVLGKKIVLVKGDSETLKSAVSETERLINEGIKLFCGEYSSS
                     RAKVATTVTEKYGAIFCVVIAVADDITERGYKYVFQPQGRATLWGELSAEFIGKAAAP
                     KLGKKPGDLRVAIAYEDSDFGTSISVAFARKAKEYQMNIVAQEPYSLSALDLSSVVLR
                     IKQANPDVLALTPYVRDGILFLQQAKDAGLSPKVLICPGGPLANDVFGEKLGDDMNYM
                     LVTTCASHDVRKEAFKPEAWEALQEFADRYNKEFKKPPTNDAYHGFQGAWTFFHFGLP
                     RAKSLNPDDVEKSMRSLVLKPGENVTTFGYQAAPQGHKNAGANLIGYPNVFQWQKGSV
                     GHLVYPPEFAKSELMLPMPLWGQRAMK"
BASE COUNT         3431 a         3919 c         4338 g         3101 t
ORIGIN      
        1 ggttaaatcc atcctgacgg aaatccccct tccgtgagga ggggggattt cccttccctc
       61 ttcccaaaaa ttctcagaaa gagcaagacg cctgattttc aaccttgaca aattcgaaag
      121 aaaagctgga aaatgcttga atctgcccag gattcaatga cgggtgcaat gtttatggag
      181 gtttacctaa ttggttgcca accctcgacc taggggggaa ttttgaaggc ggaggagaaa
      241 ggactatggg ttctgaagaa atcaccagaa gaaaattcat cgagaatgtg gccaagtact
      301 gcggagcggg tattgcggcc tttggcctaa tgggccccgg gaaagctcat ggggtcgcgg
      361 acttggactc cccggcccac gacggggttt ggcggatgcg ggattctgct gtcatgcaaa
      421 gcggcgagtg gagaaaaaat gcgcccatcc ccccggatta tttcgaccgg aagccggagc
      481 acttcatcaa cgacgggaag gcagttgaat ggtctcacct ccatgaaaat tggtatgcgg
      541 aaggggacaa atctttggtt ggcctcatca tggccaccca gctggacaag gacctcaagg
      601 gagacatccg caaggcggtc aggctcgtcg gaggcttcca gaaatccctg aaaaagaccg
      661 acaaaatcct catcaagccc aatatgaact cggtacacga atggccggga ggcgggactg
      721 atgcagcctt cctggaagcc ctgatcttca atctcaaaga tgagggattt gaaaacctta
      781 ccgtagccga cacctgcggg ccctggggac cgacggcccg ggtagcgaag tcactgggta
      841 tcgaggcggt ttgcaacagc acaaaaacga ccctgcgaga ctgggaaaag acgaaatgga
      901 tgaacattcg caacgccaag gctgagttcc tcgggaaatt cggcgggggc gatggcaccg
      961 tggcttatcc cgaagacctg agaaattacg acaagatcat ctacactccg gtaatgaaga
     1021 cccattacct tgggggtatc accatgagct tgaagctctc cgtggggatg ctgtatcgcc
     1081 cagaccgccc cgcccagctc catactttca accatctctt cgtcccccag gcggcggcag
     1141 agatcaacct gccgctccgg ccggacctga tcatcatgga tgggagaagg tctttcatca
     1201 gcggcggtcc atcccacggc gagctggtga aaccggggat gattgtcgtt tccggggacc
     1261 aggtggcctg cgatgtaacg ggtatcaaca tccttcagga gtggtatccg aatctggccc
     1321 agaacaagat taccacttac ccctggtacc agaagcaggt tcgccaggcg gtgaaaatcg
     1381 gtgtgggata tgcgaaaaag gaggaggacg tcaagctcgt cctgggatga aggcaagttt
     1441 gtaggggcga cttgtcgtgc gtttcgcggg ttcgccccta caaagttaat tgactgatcc
     1501 gagactggaa ttgaagattg caagacctct gacctttata tcaaagagaa aacggagatt
     1561 tcctgatgat gaaaacagct accctttttg ccatcctgat attcactgtt tttttgatcg
     1621 ttccggggga atccccctcc cagagttcaa cctttgccga atttaaagta gatgccctct
     1681 accgggcagc gaaagtatcc tggaagacag ccggagaccc gaaagctgaa atgtccgtgc
     1741 agatcatgcg ggcggatact ttcgaagagg gcccctacca ggaagtcggg gtggtcaaga
     1801 ttaccccggg aaagagcagc tacgaatata ttgacaagag catgggcgct gaagccaaat
     1861 attattacaa gctgattatc aaggagacga atgaatccat gggccccaaa cccacgaggc
     1921 cgttcttctc tcctccggca acctaaaaat gcggattggg caatgcggaa tccaagttca
     1981 tcctctttgt aggggcgacc ggccggtcgc cccttccctt ttgaaggctg agaaaatggg
     2041 agaatacggt caatgcccca agaagtaaac cggcgcacgg tcttgaaagg catcaccctc
     2101 ggcgctttcg gcctgggaag cgtggtggcc gtggggaacc tgctggaggt tctgaccttt
     2161 cccaacggtc agagggtggc ccttgctaag ggggtgatcc tagtggacaa atccctctgt
     2221 tccggatgtc gcgtctgcga gatcgtctgc gccaattaca acagcggagg ccgcaactcc
     2281 ccgtcccttt cccgcatcat cctggaaaag gaaatcatgg gcggtgatta tcagcctaaa
     2341 gtctgccacc agtgcgtgga cccgccctgc ctcaaggcat gtccggtcac tgccctccag
     2401 gtggacaagc agaggggaac ctacgcccgg gttatcgacg aacgtgtctg tatcggctgc
     2461 cagaagtgcg tggaagcctg cctgcagcac tttcggcccg cccgcccgag atatgatgcg
     2521 caacgccacc ggtcggtgaa gtgccatctt tgtttcggag acccccagtg cgtgaagttc
     2581 tgtcctctgg gggttctgcg gctggaaagg tcggaaaaag gtttgatggt cggatacccg
     2641 gtgatccagg aagactaaaa tctcattcac cacagaggca cagaggacac agaggaataa
     2701 aaccttgata aggtaagagc caaaatatac aaaagctttc ttttttattc attagccaac
     2761 tgaaattctt ttctctgtgt tctctgtgcc tctgtggtga aaacattttt ttatgaatcc
     2821 taaaggcgga tgggtcggaa aaattcttcg ggttgacctg acttcaggaa agacttctga
     2881 gcagtccacc tatgattacg tccccaagtt tatgggcggg cgcggcctgg gagccaaggt
     2941 ctgctgggat gaggtccctc ctgaggttgg agccttcgac ccggaaaacc ggcttatcct
     3001 ggccactggg ccactccagg gaaccctggc acccacctcc ggaaggttca tggtcctggg
     3061 caaagccccc cagaccggcc ccatcgaatc cttctgccgc tccggcgtgg gagggcactt
     3121 cgcccctgaa ctgaagtggg cggggtatga tgcggtcatc atccagggga aagccccgaa
     3181 accggtttac ctttggatca ccgaccacaa ggccgaaatc ctggatgcca cccgcctgtg
     3241 ggggctcgac acttaccaaa cccagcaaat gatctggaag ctccacggtc ccaagacccg
     3301 ggtaatggtc atcgggcggg ccggagaaac caaatcccgg attgcctgca ttctgaccga
     3361 caccggggac gcatcgggcc agggtggcta cggcggggtc atgggctcca agaacctgaa
     3421 ggccatagcg gttcgcggaa ccggtacggt gaatgtggcc cggcccaaag atttaatgga
     3481 aatcacccgc cacatccaca gcctcttttc ccgcaagtcc ctgaaaagcg acccttacca
     3541 gccggaagag aaaaatttca agtacaatat ctggggcggg gggtatgggc ggggaagtct
     3601 gtccctcctt cccggagaac tgctggacct ctgcaactcc ccttcttcgg gctactccca
     3661 aactccggac gggtgtttcg cctgcccggt ctcctgccgt tcccgggtga agggcccgga
     3721 catcaccccg ggcgtagcct tttgcgccca gtcctacatg tacctggagt ccatggtcca
     3781 tcagccggaa aagggctaca gcaaggtcac ctggcaggcg gccaagctgg gcgaccttta
     3841 cgggatcaac gcttacgaac tcatggccat tatcccctgg ctgtctgatt gcttccggga
     3901 gaaattgatc aaggaggaag aagttggact tccgttgaaa gagatcggct cctgggattt
     3961 tatctccaag ctcctgtaca agatggccaa ccgggaagga attggagacc ttctcgccga
     4021 aggaggccag cgggccgcgg ccaagctggg cggaaaggcg gagaagcttt cccacatgta
     4081 ttatccgcgg gcgggaaagt tcgctggata ccgggagcat tgggtttacc tggggggatt
     4141 cccgaccggt tatgcggtcc cccaattggc tctgatgtgg gtgctggaca accgggatgc
     4201 catggtatcc cacagctata tctctgcgct ttggggagcg gcctttaccg tcggccaaaa
     4261 cgcccttacg gctgttcccg aaaaaattat ccctgtcctg aaacccgtga tgaaagccgc
     4321 ctacggatcg gcggaagccg ctgaattcgt gcgaccggac gggaaaggcc tcaattggaa
     4381 atgggccgcc cccgtggtca agcggtatca cgagcattcc ctcctaaaag actcttacat
     4441 tctatgcgat atcctctttc ccttcctctt cgatgccaac acccccgacc atgtgggcga
     4501 cctgactctc gaatcccgca ttttctccgc cgttaccgga agggagctga acctagaaga
     4561 gtcctacaaa atgggagaga tgctcagcac gctggaaagg tcgctggcag cgcgggacgg
     4621 acgtgcccgt aaggatgata tcctgcacga catttatttc gagaaggaag atgccggggg
     4681 gcggaaatac aaacgggaag acgtggaacg ggccaagagc gagtattacc aacttatggg
     4741 gtgggatgcg aaagcggggg tccccacggc agggacgctc gaaaagctgg ggctgaaaga
     4801 agtagcggag gggctggaga aaaggggact cctggggaaa aaatgaaatt cccccccttt
     4861 gtaaaagggg ggcagggggg atttcctcaa gacggctcaa agcaaatccc cctatttccc
     4921 cctttttcaa agggggattt gaaccccaca aataatttaa ggatgagctc ttaatatcgg
     4981 aatggaaaat gtaggggcgc gcggcgcgcg cccttgtagg aaataccaca gatgagcgag
     5041 acttattctc ttccggcctt cactatgacc gactacaacc tgtaccgggt ggacgaccgg
     5101 attttctatg tgttcatcct tgccgctctg gccttctgcc ttatccatgc cgcccgccgg
     5161 gccttccgcc agcctaagcc cacggaggtc ccttccctca aatccacctc aggcaacgaa
     5221 gagcagatgg agcgacatac ccttttccag aggatttttc actgggccaa tgcgattgcc
     5281 atttttatcc tggccggatc aggatggatg atctatcaac ccggccgaat ccctttgatg
     5341 gaaggacccc catcggaatg gttcctctgg caccgttggg gagtcgcttt ccttctggcg
     5401 ggaatcgcgg cccatatcat ccgcgaggcg tttctcgccc aagaggcgaa ccccatggtg
     5461 ttcgaccgtg ccgaggtgcg aaggattcta tccatcttca aaaacttctt cggcctttcg
     5521 aagaactatc ccctggccgg aaagtatcat accggccaat tgatcttcca ctgggccgtg
     5581 gctgggaacc tgttcctcct aatcctgacc gggatggtcc tctggaagcc tctccggggt
     5641 tttcttcccc tatcgctctt cggcctgggg tgggatttca tctatttcac gcgcattctt
     5701 cacggtttct tctccgccac ccttttcgcc agcctgatcg gccacatcta tttcgccctc
     5761 ttcatcaaaa agaactggcc cgaagccaag tccatggtca ccggccgcat gcccctgcag
     5821 gagtatctca cctcccattc cctcccggac tagccaagaa tgtcagaagg tctttccctt
     5881 tcaacggaag cccgaggagc gaagaaaggg gactggcctg cccccacttc ccttttacga
     5941 tcctcttttc tgctaaaaaa ataggcttac ggcccgggag gaaggatgtt gcgcggaaaa
     6001 agcatcggcg ggaaaagtcc cgctcggagg ttctcccgtg cttccgcaga atggggcggg
     6061 cccatcaagc tcccattcgg acgcccctac atcggaatac ctctgttctt agggaaagaa
     6121 cggcttgccc gcccggtcca agaaagggag gcgtgcctca tggtttttcc gcgcaacgtc
     6181 cttccttccg ggccgggcaa tgcctgaaat tcaataggcc tacgcctatt cgtggaaaag
     6241 tggcgggggg ggcaagaggg gatatatact tgcaaatgtc ccttttcctg gcttaatatc
     6301 tcgattgaat ccccagtcat tgcattcttg gaaaggagtt aagaagaaat ggctgaaaaa
     6361 taccctcacc ttttttcgcc cgtaactatc cataccatga ccctgaagaa ccgggtcgtc
     6421 atgcccccca tgtgcaccga ctatgcgacc atcggcggag cggtgaacga tcgattgatc
     6481 gaatattatg cgaccagagc ccgcggaggg gttggattga tcgacgttga attctcctat
     6541 gtccatcccg ccggcaaggt cttcgagcac atgctgggaa tctacgatga caagttgatt
     6601 cccggcctcc gcaggttaac cgacgccatc catcaaggcg gagccaaggt tatcgttcaa
     6661 attgcccacg gcgggagaag gggtcattcc gacatcaccg gggcgaccat agtgggccct
     6721 tcctccatcc cccggctgaa cggggagacc cccaaggaat tgagcccggc cggaatcgag
     6781 gacctgatcc aggctttcat cctggctgcg agacgggcga agaaggcggg gtttgacggg
     6841 gtcatgatcc atatggccca cgcgtacctc ctccagcaat tcctttctcc cttcagtaac
     6901 gtccggacgg accgctacgg cggggacctg gaggggcgcg ccagatttcc cctcgaggtc
     6961 gtcaagggcg tccggcggga agtggggaag gattatccga ttacctgccg gctctgcggc
     7021 gatgaattcc tgaaagaggg gttcgacctg agccagagca tccgggtggc gaagatgctg
     7081 gaggccaacg gagtcaatgc cattgaagta tccgccgggg cccatgaaac cggctatgtc
     7141 ataaccgccc ctccttatta ccccatgggt tttctgtctc ccctatccga agccatccgg
     7201 aaagaggtga acattcccgt cggggccgtc gggaggattc acaccccgga ggtggcggaa
     7261 gaccttctgc ggcaggggaa ggccgacctg atcgccgtcg gccgcggtct catcgccgat
     7321 cccgactggc cgaaaaaggc acaggaaggc cggccggaaa cgatccgccc ctgcatctcc
     7381 tgcaatcagg ggtgcggcga ccggatgtat ctccaccggg atatcagctg tacggtgaac
     7441 ccggccgtag gccgggaagg gactttcccg gtggaaccgg cccggaagaa aaagaaggtt
     7501 ctcgtccttg gcgggggccc ggcgggattg gaggtggccc tggttgcggc ccggagggga
     7561 cacacggtcc atctttatga aaaggaaaaa gagctgggcg gccagctcaa catcgccgcg
     7621 gttcccccgg ccaaggagaa gatccgggaa ttcaaagaat tcctcatccg cgaagtaaaa
     7681 ggtctgccga tcaaagtggt gcatgcaaag ccggacgcca gaaccttgaa gaaaatctcc
     7741 cccgatctgg tggtggttgc ggtaggcggg aagcccaaag taatggaagg gctaggtttc
     7801 gatgaccgga aggtgatctc cgcatgggat gcgctatcgg gcaaaaagcc ggtcgggaga
     7861 cgagtcgtga ttgtcggcgg gggccaggtt ggcctggaga ccgcccactt tttcctggaa
     7921 aagggaaaag acgtcactgt cctggagatg ttaaaaagcg cggggcagga tatgagtccc
     7981 cgggcgagaa agctgatcct agaaaaattg gtccagggcg gggcggaggt cttgaccgag
     8041 gcaaaggccc tttcggtgga gaagggggac ctgaccttcg aacgggccgg cctgagggaa
     8101 aaaatcaaag gggcggattc catcatcatc gccgtgggaa cggcctccca ggatgaagag
     8161 attccgggat tgaaaaggtc acgaatcccc ttccggcggg tcggggatgc ttcggccccc
     8221 cgaaagctta tcgaagccgt ccacgaaggc tatcaggtgg gagtcgaaat ttaggcgctt
     8281 agcgactcat taatcaacct attggtaaga aggccttatc ctccatcacc cccgccctga
     8341 ccctctcccg tcgaagggag agggaaccgg aatgaaatct tacatatctt tggctaggtt
     8401 ttttctgtga agttcaggta gaatgcactg tgaagtgatt ctgaataggg agaggcgagc
     8461 atgaacatga tcgaaaagct gccggaattg gtcaacggca atgaacggct tgtatggcgg
     8521 gggcggtttc ttacggttga ctttctgatt gaggtaggcg atgtttctta cctggtctcc
     8581 atccaggcgg gacgtatcgt gtccgtggaa cagggtccct tttacatgcg gccatggcgt
     8641 tttgcagttc gggcatcggc cacagcctgg gaaaagctat gggagccgat gccccgtccc
     8701 ggatacacgg acattctagg cttaatgagg cgcggcgagg cgcgcatcga aggcgatttg
     8761 cagccgctcg tagccaatct tcgttacgtc aaagaagtcc tggttacacc gcgacggctg
     8821 aagggaggtt gttgacatgg ctgctcagat tgagccaatc gtcggtcgct atattcaact
     8881 aagattgggc gggagggcct gtcgaatcta ttttgaggag accggcaagg gaatccccct
     8941 ggtttgcctg cacaccgccg gttccgacac ccggcagtac cggcacctga tggtcgaccc
     9001 ggcggtgacc gaacatttcc gggtgctggc ctttgacttg ccctggcatg gcaaatcgaa
     9061 tccgccgccc ggttggcacg aggaggaata ccgcctgacc actgaggcct atctcgaagc
     9121 gaccatgggc ttttgtggag ctctggaact taatcgcccg gtggtgatgg gttgttcgat
     9181 gggggggcgc atcgtcattc cccttgcggc tgagtacggg aaggaattgc gcgcggtcat
     9241 cggccttgag gcaggctatc aagtgcagcc ctggttcgac aacacctggc tgcaccatcc
     9301 ggatgtacac ggcggtgaaa tttgtgctgc ccgggtctcc ggattaatgg ccccgcaaag
     9361 cccggaggaa acgcgctggg aaacgctctg ggggtacatg cagagcgggc cgggcgtttt
     9421 caagggagac ctctactttt atttccagcg ggccgaatcg gatttcctaa attatatggc
     9481 ccgtattgac acgaaagcct gtccggttta tcttttggcc ggcgagtacg actattcctg
     9541 tacgccggag gatacgcagc gcacaggagc ctatatcccg ggctccgaag tgatgatcat
     9601 gaaagagatg ggccactttc ccatgtccga gaatcccgtc gctttccgtc gttacatcct
     9661 tccggtcctg gaaaagatcc gcaaccagaa atgaacttgt tgagaaccta tcctgtttta
     9721 attctcttcg ggaaaaccgc cggcttaaag ctttgccgtc ggtcgcggga ctggtttttg
     9781 atgattcccg actagacggg gaaagggaat tgggaataat tttagaaagg agaggaacat
     9841 gaagagtagc agaatgattt ccttggttgt cgttctgctg gcttgcctgg ctatgggcac
     9901 acaagtccgg ggggcggata atttcccggc gaaacctctt gagctcgtgg ttccctacgt
     9961 cgctggaggg tcgacggacg tgatgtgccg caccattgtg gcccgggctc tccccttctt
    10021 taacaaccag ccggtcatgg tggtgaacaa acccggaggg gggaccgtga tcggttcccg
    10081 ttatgtcctg gataaccgaa atgacggcta caccctctac tccacctcca ccgcgagcat
    10141 gatgatcgcc ccggtgatca acaagaccaa cttcagttgg agggatttca ttggcgttgc
    10201 ccaaaccatg ctgggctcgg atgctttata cgtaccgggg gatgcttctt acaacaccct
    10261 tgagaagttc atcgattacg ccatgcagaa cccgggaaag atcaagtaca ccaccgccgg
    10321 cacggggggg tcttcccacc tgcccatgga ggggtttgcc gtggccaagg ggctctcgtt
    10381 aaagcatatt cccaccaagg gggatgccga aaacatcgcc gcccttatgg gggggcatgt
    10441 gaccgccggt gccgggaatc ccatcgcatt tcaaccccat gtcgcatccg ggaagtttaa
    10501 gtgtctggtc cagttcgggg cggagcggga caaggccttt ctcccgaata ttccgacctt
    10561 caaagagttt ggaatagatg tagctgtcga cctctggcgc tgggtcgtag tgcccaaagg
    10621 ggttcccccg gaacgagtca agttcctggc tgaggcgttc aaaaagatct ttcaagataa
    10681 ggaagtcctg gccagcctgg aaaagataca gtgcccggcc tcctatcttc ctccggaaga
    10741 ttatgaaaag gccatgaagg cgagtgaagc ctccatccta ccgcttatca aggcttccaa
    10801 aatcatggaa gcaaagtaag gaaagacggg aaatatttta ggtttgaaag atgcaaggtg
    10861 tatggtgtaa atggttctaa cgccaggcct gcattataag gagtaatcaa gtctgaaaaa
    10921 ggagagagtc catgaaaggg tacaacggaa aaattctgcg cgtaaacctg agcacccgcc
    10981 aggtttcaac ggaggcgccg actgaagagt actataaaac ctacctgggt gggcgtggat
    11041 tcatcgtcca tacccttttg aaagaagttc cccggggggc ggacccgctg gggccggaga
    11101 acaagctcat ttttgctctc ggtcccatta ccggccatcc ttttccgggg agcggcagga
    11161 acagcgtagg agccaaatcg cccctcagcg ggggattcgg ggaaagcgag gccgggggct
    11221 tctggggcgc ggaattgaaa aggtctggct acgacgccat tatcgtggaa gggaaggcgg
    11281 ataaaccggt ttacctttgg atcaaggacg gacagacgga aatacgcgat gcccgtaatc
    11341 tctgggggtt ggatgtggcc gagaccgaga aagccattca ggaagagctc caagacggta
    11401 gggtgcgtac ggccacgatc ggggtagcgg gggagaaact ggtgcggttt gcctccattt
    11461 gcaacgacat ctcccacgtg gccgggagga cgggaatggg ggcggtcatg ggctccaaga
    11521 agcttaaggc ggttgccgtc agggggaata ctcctccgga gatgggaaac cgggaaaagg
    11581 tcctggaact ctcgcggtgg atggggcaga acttcaaaac taagaccaat ttctggcagt
    11641 atggtaccgg agccatgatg gattattacg aagcctctgg aaatctgccc atccggaatt
    11701 ttgccgccgg ccggttcccg gaagtggaag acattacctc ccagagcatg tacaagaagg
    11761 gctacgtaga gaagatggaa agctgctatg gatgcccagt gcgctgtaag aagagggtaa
    11821 agctggagca gccttggaag gtggacccga tctatggagg ccccgagtac gagaccctgg
    11881 gggctttcgg gtcctgctgc gggatcggcc aggtcgaggc cattatgaag gccaatgagc
    11941 tttgctgccg gtatgggatg gacacaatct cggcgggggt cacgatttct ttcgccatgg
    12001 agtgctttga gaaaggagtc ctgactctga aggatacgga tggtctggag ctcaccttcg
    12061 gcaatgcctc ggccatggtg gagatggtgg agcggattgc gttgcggaaa ggcctgggaa
    12121 atctcctggc cgaagggacg aagagggccg ctgaggtcat cggcaaaggg tcggcggaat
    12181 gggccatgca cgtcaaaggc gaggaaatcc ccatgcatga gccgcggtat aagcagggga
    12241 tggggctgca ctacagcgtc catgccaccg gggccgacca ctgttcgggg cctcatgatg
    12301 atgtggtcaa caagaaactg ccggaatggg accggatcgg cctggccgag tccataccgg
    12361 tgtatgaaat gagcgggcgc aaagcccgga tggtttacga ggtgggcctg tggcgacatg
    12421 tggccaattc tatcggtctt tgtctctttg tgccctggag caacgagcag gttacccagg
    12481 cggtcgaagc gatcaccggc tggcccatta gcttctggaa gctcatgaag accgccgagc
    12541 ggtccatcac cctggcccgg gtcttcaacc tccgagaggg gttcacacac cgggatgata
    12601 cactgccaag gaggtttgcc tcttccccgg cggacagtaa tttgaaagga gtgggagtgg
    12661 acccggagaa gctttcctcg gcccagaagg tctattatca aatgatgggg tgggacgaga
    12721 acggtgtccc cactcgcgca aggctcatag aattgaatat cgaatgggcg tcccagtatt
    12781 tgccggcggg gcgataaccg cccgcactaa aggtggcctt ccaccgggtg ctaggcctgg
    12841 gggcaaggac cttctgggca aaaaccatgc ggcatctttg attccatgga acgggcgggc
    12901 ctcggaattg cggaatgtcg attgcggaat gcggattgaa aaagaaaacc ccaaaatccg
    12961 aaatccgcaa ttcgaaatcc gaaatgggaa ggccgatgct ttttgcccag aagctcctcg
    13021 cccttaggct acaacccaag cttcccggaa aaatgtcaaa ctttgggttc ctcggcagag
    13081 atgggggttt agcctgaatg aaattaaaag cctgtaccta aataatattt cctcctttgt
    13141 aaaagggggg gagggggcat ttccccaagg cgtctcaaag taaatcctcc tatttccccc
    13201 tttttcaaag gggggatttg aagcccgcag ctaatttaag gataagctct aaataaaacc
    13261 agaaaggaga agggtatgaa gcgattcttt tttctttcct tcgtgctgat cctgaccatc
    13321 cttccgttgc aggcgatggc ccaagacaac gtgaagatcg gatgcgtcct gccttataca
    13381 ggggccctag cttggcaagg gcaggagacg tttcgaggaa cccagatcgc cgcggatatg
    13441 cagaacaaaa gagggggagt tctggggaaa aaaatcgtat tggtcaaagg cgattcggag
    13501 acgttgaaat ccgcagtgtc ggagacggaa agattgatca atgaggggat caagctcttc
    13561 tgcggggaat actctagttc gcgggccaag gtggcgacga ccgtcacgga gaaatacggc
    13621 gccattttct gtgtggtcat cgccgtggcg gatgatatta ctgaaagagg ctataaatac
    13681 gtttttcagc cgcagggaag agccaccctc tggggcgagt tgtccgccga atttattggc
    13741 aaagcagcgg ctcccaaact gggtaagaaa cccggcgacc tccgagtcgc catcgcctac
    13801 gaagattccg actttggcac cagcatcagc gtcgcttttg cccggaaggc caaggagtat
    13861 cagatgaata tcgtggccca ggagccttat agcctttcgg ccctcgacct ttcttctgtg
    13921 gtgctaagga tcaagcaggc taaccccgat gtcttagcgc ttacgcccta tgttcgggat
    13981 ggaatcctct tcttgcagca ggctaaggat gccgggttaa gtccgaaggt tctcatctgc
    14041 cccgggggcc ctctggccaa cgatgtgttc ggggaaaagc tgggcgacga tatgaactac
    14101 atgctcgtta ccacgtgcgc ttcccatgac gtccgtaagg aagctttcaa gccggaggca
    14161 tgggaggccc tgcaggagtt cgccgatcga tataacaaag agttcaagaa acctcccacg
    14221 aacgatgcct atcacgggtt tcagggcgcc tggaccttct ttcactttgg tcttccccgg
    14281 gccaaatctc tcaatccgga tgatgtagag aaaagtatgc gcagcctggt cctcaaacca
    14341 ggagagaacg tgacaacctt tggctatcag gcggctcccc aaggacacaa gaatgcaggc
    14401 gcgaatctga tcggctaccc caatgtattt cagtggcaaa aaggaagcgt cggtcatctg
    14461 gtttatcccc cagaattcgc caaaagcgag ctgatgctcc ccatgccctt gtggggacaa
    14521 agggctatga agtaatttcc ttggctgttt gagtagggag ccattcttcc ccctgcacag
    14581 ggaagctgtg aaaaaattga aatattcccc ctcaccctac cctctcccgc gaggggagag
    14641 ggtaacctta ttgaaatagt ttttaagaat tccctccccc tcgacggggg agggcgaggg
    14701 tgggggtgat tttaggtaat ttttcacacc ttcaggcaag agggaagaat gaatccttcc
    14761 gtccattcca caaaagggtt ctatgatcg
//
LOCUS       DASPRR010000476         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_601507, whole genome shotgun sequence.
ACCESSION   DASPRR010000476 DASPRR010000000
VERSION     DASPRR010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601507"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VEL68_17525"
     CDS             <1..487
                     /locus_tag="VEL68_17525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37827.1"
                     /translation="VSSSATIGSFSFGTYEFIYVGAAILVLAGLLLFLKRTPTGQAAL
                     AVGQNPRGAKLVGIHVDRTYLLIFSLSIALIGALGAIFMTRHSIFPLVGAPFTMKSFC
                     LIAMAGIGNLTGILWCSLILGLAENFVLSFRGYAGWADILFFALIVLVIMVRSHQREV
                     K"
     gene            484..1410
                     /locus_tag="VEL68_17530"
     CDS             484..1410
                     /locus_tag="VEL68_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37828.1"
                     /translation="MKKSRPLLYLVVLAILFTLPFYTGVYVLNVMDIILIYMALALSW
                     DMLLRSGQISFGMAGFFGVGGYAAVLLFLNTGVHPLLSIVLGGLAAGVLAWLIGSAVL
                     QLRGMYFAIVTLALAEIFRVIVRNLPDLTGGPEGTVLSQAIFAGNSTYTYWLVLAAAA
                     LSVVVSEVFRHSRIHFALTSIRNDEIVAKSSGIDVFKFLVFLFAVTSAIQGITGGAYA
                     HIYGFVSPEGSFSVDFTLLPIAMALLGGMHSTWGPVVGAVVLGVVSEFLKLQIPYGHL
                     MVYGIIIVVAILFFPQGIVGTLGQKMNKTGSP"
     gene            1423..2139
                     /locus_tag="VEL68_17535"
     CDS             1423..2139
                     /locus_tag="VEL68_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37829.1"
                     /translation="MAILRTEKLARHFGGLAALSGVSIEFRDNEVLGIIGPNGAGKTT
                     FINVIAGIYLPTEGKVFFENQDVSELPAHQRCHLGIARTFQLVRPLPGLSLVENVMVG
                     GLFGKGMTQKEARRKAEELCGFLGLSDLGREITRMTALEVKKMEIARALATQPKLLFL
                     DEVMAGLNSEETMLMIDLVRRIHRQGITIGIVEHVMRVIRELTDRVVVLDWGELIAEG
                     PYGDVSNDPKVISAYLGEEA"
     gene            2139..2840
                     /locus_tag="VEL68_17540"
     CDS             2139..2840
                     /locus_tag="VEL68_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37830.1"
                     /translation="MLGVKNINIFYGKLHILWDLSLEVGKESVGLFGPNGAGKTTLIN
                     AVLGLVKPVSGEVQFNGEALLPLETFQRIRRGISVVPQERELFPLMTVLENLRSGATY
                     IPEAKEKVQENLDLVLGIFPALKGRLKQLAGTLSGGGQRMLAIGRALMAGPKLLILDE
                     PSGGLQPSLVSDLFHRLKEIKEKVSLLIAEQNVRQCLKAIDRGYVIENGRIVMEKNAR
                     DLAGNDHIRRSYLGI"
BASE COUNT          599 a          884 c          957 g          697 t
ORIGIN      
        1 tgtctcttct tcggccacga tcggttcttt cagcttcggg acatacgaat tcatttatgt
       61 gggcgctgcc atcctcgtcc tggccggact ccttctcttc ctgaagcgta cccccacagg
      121 ccaggccgcc ctggcggtgg gacagaaccc tcggggggcc aagctggtgg ggatccacgt
      181 ggaccggacg taccttttga tcttcagcct ttccatcgcc ctgatcggag ccctgggcgc
      241 gatcttcatg acccgccatt ccatcttccc cctggtgggg gcgcccttca ccatgaaatc
      301 cttctgtctc atcgccatgg cggggatcgg aaatctcacg gggatcctgt ggtgcagcct
      361 gattctgggg ctggcggaaa acttcgtcct ctccttccgg gggtacgcag gctgggcgga
      421 cattctcttc tttgccctca ttgttctagt gatcatggtc cgctcccacc agagggaggt
      481 gaaatgaaaa aatcgcgccc ccttctttac ctcgtcgttc tcgcgattct tttcaccctg
      541 cctttctaca caggggtcta tgtcctcaac gtcatggaca tcatcctgat ctatatggcc
      601 ctggccctga gctgggacat gctgctccgg tcggggcaga tctccttcgg catggccggc
      661 tttttcgggg tggggggata tgcggccgtg ctcctgtttc taaacacggg ggtgcatcct
      721 ctgctcagca tcgtcctggg cggtctggcc gccggagtcc ttgcctggct catcggttcg
      781 gcggtgctcc agctccgggg gatgtacttt gccatcgtga ccctggccct ggcggagatc
      841 ttccgggtga tcgtgaggaa ccttcctgac ctcacggggg ggccggaggg gaccgttctt
      901 tcccaggcga ttttcgccgg gaattcgacc tacacctact ggctggttct tgccgcagcc
      961 gcgctgagcg tagtcgtctc cgaagttttc cggcacagcc ggattcattt tgccctcacc
     1021 tctatccgca acgatgaaat cgtggccaag tcgagcggga tcgacgtttt caaattcctg
     1081 gtgttcctct ttgcggtgac ctccgccatc cagggaatca ccgggggagc ctatgcccac
     1141 atttacgggt tcgtttcgcc ggaggggagt ttttcggtgg atttcacgct tctgcccatt
     1201 gccatggccc tcctgggggg aatgcattcc acctggggcc cggtggtggg ggcggtggtg
     1261 ctgggggtgg tctccgagtt cctgaagctg cagattccct acggccatct catggtgtac
     1321 gggatcatca tcgtggtggc catcctgttt ttccctcagg gaatcgtggg aaccctggga
     1381 caaaaaatga ataaaacggg cagtccctag aaggtacatc ctatggccat tttaaggacc
     1441 gaaaagttag ccaggcattt cggcggcttg gccgctctgt ccggggttag tatcgagttc
     1501 cgggacaacg aggtcctggg gatcatcggc ccgaacgggg cggggaagac gaccttcatc
     1561 aacgtcatcg ccgggatcta ccttcccaca gaggggaaag tcttttttga aaaccaggat
     1621 gtgagcgagc ttccggccca ccaaagatgc cacctgggca tcgcgcggac cttccagctc
     1681 gtgaggccgc tgccagggct cagcttggtg gaaaacgtca tggtgggagg gctcttcggg
     1741 aaggggatga cccagaaaga agcccggcgg aaggcggaag agctgtgcgg gttcctgggg
     1801 ctttccgacc ttggccggga gatcacccgg atgaccgctt tggaagtcaa gaagatggag
     1861 atcgctcgtg ccctggccac ccagcccaag cttcttttcc tggatgaggt catggccggg
     1921 ctgaacagtg aggagaccat gctgatgatc gacctggtgc gaaggattca ccgccagggc
     1981 atcaccatcg ggatcgtgga gcacgtaatg agggtcatcc gggagttgac cgaccgggtg
     2041 gtcgtcctgg actggggaga gcttatcgcc gaaggaccct acggggacgt ctccaatgac
     2101 ccgaaggtga tctcggccta cctgggcgag gaagcgtgat gctgggggta aagaacatca
     2161 acatctttta cggcaaattg cacatcctgt gggatctttc cctggaggta gggaaagagt
     2221 ccgtcggcct gttcgggccg aacggggccg gtaagaccac cctgatcaat gccgtcctgg
     2281 ggctggtgaa accggtgagc ggggaggtac agtttaacgg tgaggccctt cttcccctgg
     2341 agaccttcca gaggatccgc cgggggatct ccgtggtccc ccaggagagg gagctctttc
     2401 cgctgatgac cgtcctggag aacttgcggt ccggggcgac ttatattccc gaagccaagg
     2461 agaaggtcca ggagaacctg gatttggtct tgggaatctt ccccgcgctg aagggccggc
     2521 tgaaacagct ggccgggacg ctaagcgggg gaggacagcg gatgctggcc atcggccggg
     2581 cgctgatggc cggcccaaag ctgctgatcc tcgatgaacc ctccggcggg ctgcagccgt
     2641 ccctggtttc cgacctcttt caccggctga aggagatcaa agagaaagtc tccctgctca
     2701 tcgccgagca gaatgtgcgc cagtgcctca aagccatcga ccggggctat gtcatcgaaa
     2761 acggccggat cgtcatggag aagaacgccc gggacctggc cgggaatgac cacattcgta
     2821 ggtcctatct gggaatctag cgaaatacca gatgctagat actggatact cgatgctgga
     2881 taaaaaaatt aaatatccag aatccagtat ctaatacaaa cgcaaaaagt aatttgtctt
     2941 tgcgaggaga ggaacgacgg agcaatcccc cccaccctct ccctccccct cgagagactg
     3001 tgtcgcaatt ggggaaaaga gtcgtcattc cggcgaaagc cggaatccag tttttgattt
     3061 tattcaattt ttcgatatcc tggatgccag ctttcgctgg catgacgaat tacgacacag
     3121 tctccgaggg gggaggg
//
LOCUS       DASPRR010000477         6559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1731149, whole genome shotgun sequence.
ACCESSION   DASPRR010000477 DASPRR010000000
VERSION     DASPRR010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731149"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VEL68_17545"
     CDS             complement(<1..1394)
                     /locus_tag="VEL68_17545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXZ37831.1"
                     /translation="MKVATAEIMRKLDRRAIEDFGIPGLVLMENAARGTVGAMFRHFP
                     GLLKMTVGILAGRGNNGGDAFAVARYLLNRGISCQVYLLAAREEVRGDAALNLEILIR
                     MGGTVSEVLNLQEWESHKEKIAAHDLLVDGILGTGLKGEVQGFFGAIIEFVNSLGTPV
                     VAIDIPSGLDADNGRVLGVCIRAALTVTFGLLKRGLLILPGARYCGKTVLVDISLPRP
                     LEGAEPLKDHLIEGAEFLPFLIPRQPEAHKGEFGHLFILSGSPGKTGAAAMVCQAALR
                     VGTGLVTLGIPESLNLILEEKLTEAMTEPLPETKGKTLGPSAFGRVMDLCNRKTALAL
                     GPGLSTHPETMKFVQKLVRSTPLPAVIDADGLNAFAEKKDGLRKSGRKLILTPHPGEM
                     ARMLGIPVEEVQQDRVHVAREFSRKNGVVLVLKGARSLVASPEGDLFINPTGNPGMAS
                     GGMGDILTGMIGGFL"
     gene            complement(1447..1854)
                     /gene="acpS"
                     /locus_tag="VEL68_17550"
     CDS             complement(1447..1854)
                     /gene="acpS"
                     /locus_tag="VEL68_17550"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXZ37832.1"
                     /translation="MIYGIGFDLVKVSRIEGALKRWGDRFQKKVFTPGEVRYCLRKRN
                     PSPNFGARFAAKEAFVKALGIGIRRGVHWRDVEVQRGPLGKPVLKIHGRAVEICMKEG
                     IEGLFLSLTHDGEYSGAMVVLEKSDQRSAVGKR"
     gene            complement(1936..2655)
                     /locus_tag="VEL68_17555"
     CDS             complement(1936..2655)
                     /locus_tag="VEL68_17555"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017323620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HXZ37833.1"
                     /translation="MPRLSVNVDHVATLRQARGGENPDPVAAALMAELAGADGIIVHL
                     REDRRHIQDRDLEVLRKVVKTKLNLEMANTEEIVQIALRIKPDMVTLVPERRQELTTE
                     GGLDVLGQQKSLKPNIDRLKKAGIRVSLFIDADPRQVSASKASGADLVEIHTGKYSDA
                     RDEEAQKKEFHRIARAISQAHKLGLRTNIGHGLDYQNVSPFSSIPQIEDYSIGHSIIA
                     RAVLVGMDRAVREMKELIKGK"
     gene            complement(2745..4097)
                     /gene="glmM"
                     /locus_tag="VEL68_17560"
     CDS             complement(2745..4097)
                     /gene="glmM"
                     /locus_tag="VEL68_17560"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXZ37834.1"
                     /translation="MGKLFGTDGIRGVANVHPMTTEMAVQVGRGTAFIFKNEKRRHRI
                     VIGKDTRLSGYMLENALVAGICSMGVDVLLVGPLPTPGIAFITSSMRADAGIVISASH
                     NPFQDNGIKIFSGDGFKLPDQLEDRIEGLIFSEQIASLRPTAQEVGKAFRIDDAVGRY
                     VVYLKNSFPKESTLEGMKIVLDCAHGAAYRVAPAVFEELGAKVIALGVDPDGENINHQ
                     CGSLYPQNVTRAVQEQGAHIGISLDGDADRAIFSDEKGNVVDGDQIMAICARDLHMAK
                     RLKKATVVATVMSNLGLDVALEEWGIRVVRTQVGDRYVVEEMARKDYNLGGEQSGHLI
                     FLDYNTTGDGVLSALQVLAVMKREEKMLSELAGILKPFPQVLLNVPVAKKKDPHQVFE
                     VASLVQKIQANLGQRGRVLVRPSGTENLIRVMVEGEKAPEISAYAEEIAECVRKNMAE
                     "
     gene            complement(4111..4920)
                     /gene="folP"
                     /locus_tag="VEL68_17565"
     CDS             complement(4111..4920)
                     /gene="folP"
                     /locus_tag="VEL68_17565"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXZ37835.1"
                     /translation="MGVLNVTPDSFSDGGRFFEWTKAVERGRQMAAEGADILDVGGES
                     TRPGSNPLPEEEEVRRVIPVIEELRGKIAIPISIDTRKARVAERALEAGAAMVNDVSA
                     LRFDEAMAGTVARWRVPLVLMHMRGQPETMQVDTHYKNLIGEILGFFEERIAYALSRG
                     VPREAIILDPGFGFGKSLEERHNLILLKQLRSFRALGQPVMVGTSRKAFIGRILNLPP
                     EEREEGTMATVAVAILNGANLVRVHEVKRVRRVVQVVDAILRSTPEQDSQF"
     gene            complement(5056..>6559)
                     /gene="ftsH"
                     /locus_tag="VEL68_17570"
     CDS             complement(5056..>6559)
                     /gene="ftsH"
                     /locus_tag="VEL68_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXZ37836.1"
                     /translation="LIAFWIFFMRQMQAGGTKAMSFGKAKVKLLSDKQQKVTFQDVAG
                     IEESKEELQEIIEFLRDPKKFTRLGGRIPKGVLLVGPPGTGKTLLARAIAGEANVPFF
                     SISGSDFVEMFVGVGASRVRDLFIQGKKHAPCIIFIDEIDAVGRHRGAGLGGGHDERE
                     QTLNQLLVEMDGFESNEGVIIIAATNRPDVLDPALLRPGRFDRQVVVPRPDVKGREAI
                     LKVHTRKTPLAENVNLQILSRGTPGFTGADLENLVNEAALLAARRNKAKVEMDDFEAS
                     KDKVLMGVERRTMIISEEEKRLTAFHEAGHTLVAKFLPVTDPIHKVSIIPRGHALGVT
                     QQLPAEDKHTLSKEYILNKIAIFLGGRAAEELVLHTQTTGAGNDLEMATEWARKMVCD
                     YGMSEKIGPLTFGKREEHIFLGREIGQPKDYSENTAVDIDNEIKRIVLENHERAKSII
                     LQQMDRLHHLATALLEKEVLAGEEIDQVLGLKPAPPESPPSNLIPEARAF"
BASE COUNT         1280 a         2025 c         1765 g         1489 t
ORIGIN      
        1 agaaatcctc cgatcattcc ggtgaggata tctcccattc caccagaggc catcccggga
       61 ttccccgtag ggttgatgaa caggtcccct tcggggctcg ccaccagaga gcgggccccc
      121 ttgagtacca ggaccacccc attcttccgg gagaactcac gggccacgtg gacccggtct
      181 tgttggactt cctccaccgg aattcccagc atccgggcca tctctcctgg gtgaggagtg
      241 agaatcaatt tccggccgga tttccgcaat ccatcttttt tttcggcaaa agcgttcagg
      301 ccatcggcat cgatgaccgc cgggagcggg gtggaccgaa ccagcttttg gacgaatttc
      361 atcgtttcgg gatgggtgga aagtcccggg cccagggcca gagcggtctt ccggttgcag
      421 aggtccatga ctctgccaaa ggcggaaggc cccaaggttt ttcctttggt ctcgggaagg
      481 ggctcggtca tggcctcggt aagtttctct tccagaatta agttgaggct ttcagggatg
      541 cccagggtaa ccagcccggt ccccactcgg agggcggcct ggcagaccat ggccgcggcc
      601 ccggtctttc cgggcgatcc ggacaagatg aagagatgtc cgaattcgcc tttatgggcc
      661 tcaggttggc gggggatcaa aaagggcaag aactcagccc cttcgatcaa gtggtccttt
      721 aggggctcgg ctccctccaa gggccgggga agggaaatat ctaccaaaac cgtcttgccg
      781 caatatcggg ccccgggaag gatgagaagc ccccttttga gcaagccgaa ggtaacggtt
      841 aaagccgctc ggatgcacac cccgagaacc cgaccgttgt ccgcatccag ccccgaggga
      901 atatcgatgg ctacgacggg agtccccagg gaattcacga attcgatgat cgccccaaag
      961 aatccttgca cctcgccttt caggccagtt ccgagaatcc cgtccacgag gagatcatgg
     1021 gcggcaattt tctccttatg cgactcccac tcctgcagat tgaggacttc ggataccgtc
     1081 ccccccatac ggatcagaat ctccaggttg agagcggcat ccccgcgcac ttcttctcgg
     1141 gcggccagaa gatacacttg gcaggaaatt ccccgattca gaaggtatcg ggccacggcg
     1201 aaagcgtccc ccccgttgtt tccccggccg gccagaattc ccaccgtcat tttcaaaagg
     1261 cctgggaaat ggcggaacat ggcccctacg gtcccccttg cggcgttttc catcaggacc
     1321 aggccgggga tgccgaaatc ttcgatggcc ctccggtcca atttcctcat aatttctgcg
     1381 gtggcaactt tcatatccca acctcgcagc agactactgt cagctatcag ctttcaataa
     1441 aaaacatcat cttttgccga ctgctgagcg ctgatcgctt ttttcaagta ccaccatcgc
     1501 cccgctgtac tccccgtcat gggtgaggct cagaaagagt ccctcgattc cttccttcat
     1561 gcatatttcc accgctcggc catgaatttt caagaccggc ttccccaggg gtcctcgttg
     1621 cacttccacg tctctccagt gaacccccct gcggattccg attcccagcg ctttcacaaa
     1681 agcttctttg gccgcaaagc gggcgccaaa attgggagag gggtttctct tcctgaggca
     1741 atagcggacc tctcccgggg taaaaacctt cttttgaaac cgatcccccc agcgcttcag
     1801 agccccctct atgcggctca ctttgaccag gtcgaacccg atgccgtaga tcataaaagc
     1861 agcgatcagc tttcagcctt tagcgatcag cggtcagttg aaacattttt tgctgaaagc
     1921 tgatcgctga aagccttatt ttccttttat cagttccttc atctcccgta cggcccggtc
     1981 catgccgacc agaaccgccc tggcaatgat gctgtgtccg atgctatagt cctcaatttg
     2041 ggggatcgaa gaaaagggag aaacgttttg gtagtccagg ccgtggccga tgttcgtcct
     2101 caggcccagc ttgtgggcct gggaaatggc ccgggcaatg cgatggaatt ccttcttctg
     2161 cgcctcctca tcgcgcgcat cactgtactt tccggtatga atctccacca ggtccgctcc
     2221 cgacgccttg ctggcgctga cctggcgggg gtccgcatcg atgaaaaggc tcacccggat
     2281 tcccgccttt ttcagccggt cgatgttggg cttcaaggat ttctgctgac ccaggacatc
     2341 caggcctccc tccgtggtca attcctgcct gcgctcgggg accagggtca ccatgtcggg
     2401 ttttatgcga agagcgatct gaacgatctc ctcggtgttg gccatctcca aattgagttt
     2461 ggttttgacc accttccgca ggacctccag gtcccggtcc tggatatggc gccggtcttc
     2521 cctcaggtgg acaataattc catccgctcc ggcaagctcg gccatgaggg ccgcggccac
     2581 cggatcgggg ttttctcctc cccgcgcttg ccgcaatgta gccacatgat ccacattcac
     2641 tgaaagtctg ggcacggata cctccctgga aacagcggtc tgctttccgc gttcggcgaa
     2701 caatcatttt ccggctgagg ggatcgttga ctgttgaggg ctccttattc tgccatgttc
     2761 ttccggacgc actcggcgat ttcctcggca taggcggaga tctccggggc tttctccccc
     2821 tccaccatga cccggatcag attttcggtc cccgaagggc gaaccaggac tcggcccctt
     2881 tgccccaggt ttgcctgaat cttctggacc aaggaagcga cctcgaaaac ctggtgaggg
     2941 tctttcttct tcgccacggg gacgttcaac aatacctggg gaaaaggctt caggatgccg
     3001 gccagttcgg agagcatctt ctcctctctt ttcatcaccg ccagaacctg cagggcagaa
     3061 agcaccccgt ctcccgtggt attgtagtcg agaaaaatca ggtgtcctga ttgttccccc
     3121 cccaggttgt agtctttgcg ggccatttct tccacgacgt aacggtctcc cacctgggtc
     3181 cgcacgaccc ggatccccca ttcctccagg gccacatcca gccccaagtt gctcatgacc
     3241 gtggccacca cggtagcttt tttcagccgt ttggccatgt gcaggtcgcg ggcgcagatg
     3301 gccataatct ggtcgccgtc caccacgttc cctttttcat cggaaaaaat ggcccggtcc
     3361 gcatctccat ccagggaaat cccgatgtgg gcgccctgct cttgcactgc ccgggtgacg
     3421 ttttgaggat aaagggaacc gcattggtga ttaatattct ctccgtccgg gtccaccccc
     3481 aaagcgatga ctttggcccc cagctcttcg aagaccgcag gggctacccg gtaggcggct
     3541 ccatgggcgc agtccagcac gatcttcatt ccttcgaggg tggattcctt gggaaaagag
     3601 ttcttcaggt agaccacgta tcttcccacg gcatcatcga ttcgaaaagc ctttcccacc
     3661 tcctgggccg ttggacgcag ggaggcgatt tgctcggaga agatcagccc ttcgattctg
     3721 tcctccagct ggtcgggaag tttaaatcca tccccggaaa aaatcttgat gccgttgtcc
     3781 tggaaggggt tatgggaagc ggagatgacg attcccgcat cggcccgcat ggaagaagtg
     3841 atgaaggcga tccccggtgt aggaaggggg cctaccagga gcacgtctac ccccatggaa
     3901 cagatgccgg ccacgagggc gttttccagc atataccccg aaaggcgagt gtccttgccg
     3961 atcacaatgc gatggcgccg cttctcgttt ttgaagatga aggcggtccc ccgacccacc
     4021 tgcacggcca tctccgtggt catcgggtgg acattggcca ccccgcgaat tccgtcggtt
     4081 ccgaaaagtt ttcccatgcc ctccactcct ttaaaattgc gaatcttgct ccggcgtgct
     4141 gcgcaaaatg gcgtccacca cctgaaccac ccgccgaacc cgctttacct cgtgaacccg
     4201 aacgaggttc gccccgttca ggatggctac ggccactgtg gccatggttc cttcctccct
     4261 ttcttccgga ggaaggttga gaatgcggcc aatgaaggcc ttgcgcgagg tccccaccat
     4321 gaccggctga cccaaggccc ggaagctgcg gagctgcttg agaagaatga ggttgtgtct
     4381 ctcctccaaa gattttccga acccgaatcc cgggtcgaga atgatcgctt cgcgcgggac
     4441 tccccgggag agggcgtagg cgatccgctc ctcgaaaaac cccagaatct ctccgatcag
     4501 atttttgtag tgggtatcga cttgcatggt ctcgggctgg ccccgcatat gcataaggac
     4561 caaaggcacc ctccaccggg ctacggttcc ggccatggct tcatcaaatc tcagggcgct
     4621 cacatcgttc accatcgccg cccccgcctc cagcgcccgc tccgccaccc gggccttgcg
     4681 ggtatcgatg gaaatgggga tggcgatctt gcccctaagt tcctcgatga ccgggatcac
     4741 ccgtcggacc tcttcctctt cggggagcgg attggacccg ggacgggtgg attcccctcc
     4801 cacatccagg atatcggctc cttctgcggc catttgtcgt ccccgctcca ctgctttggt
     4861 ccattcgaaa aaccttcccc cgtcggaaaa agagtcgggg gtaacgttca aaacccccat
     4921 gatcaaggtt cgttgggaaa ggtcatattc cgtttcgcgg aaacgaagaa tgggcgatcg
     4981 cataataaat aagggtcgaa ccccaaggat caagccggaa tccgcccccc ttgatccttg
     5041 acctacgacc cttttctaga aagctcttgc ctcgggaatc agattggaag gagggctttc
     5101 cggtggagcc ggcttcagtc ccaaaacctg gtcgatttct tcaccggcca gtacttcctt
     5161 ttccagaaga gccgtggcca aatgatgaag cctgtccatt tgctgaagaa tgatgctctt
     5221 ggcccgctca tggttttcca ggacgatccg tttgatctca ttgtcgatat ccaccgcagt
     5281 gttttcgctg taatccttgg gctggccaat ctcccggccg aggaagatgt gttcttctcg
     5341 cttgccgaaa gtgagggggc caatcttttc gctcatgccg tagtcgcaga ccatctttcg
     5401 ggcccactcg gtggccatct ccaggtcgtt gccggccccg gtggtctggg tatgcagcac
     5461 cagctcttcc gccgcccggc ctcctaggaa aatggctatt ttgttcagaa tgtattcctt
     5521 ggagagggta tgcttgtctt ccgcgggcag ctgctgggtg actcccagcg cgtgtccccg
     5581 gggaatgatg gaaaccttgt gaatggggtc ggtcaccggc aggaacttgg ccaccagggt
     5641 gtggccggct tcatggaagg cggtgaggcg tttctcttcc tcgctgatga tcatcgtccg
     5701 ccgttccacc cccatgagca ccttgtcttt ggaggcctca aagtcgtcca tctccacttt
     5761 ggccttgttg cggcgggcgg ccaagagtgc ggcctcgttg accaggttct ccaggtctgc
     5821 cccggtgaat cccggggttc cccgggagag gatctggagg ttcacgtttt cggccaacgg
     5881 ggttttccgg gtatgcactt tcagaatcgc ttccctaccc ttcacatctg gccggggaac
     5941 caccacctgc cgatcaaacc ggcccggccg cagtagagcg gggtcgagaa cgtccggacg
     6001 gttggtggcg gcgatgatga tgactccttc attggactcg aatccatcca tttccaccag
     6061 gagctgattc aaggtctgct cccgctcatc gtgccctccg ccgaggcccg ctccgcggtg
     6121 acgtccaacg gcgtcgatct catcgataaa aataatacag ggagcatgct ttttcccctg
     6181 gatgaaaagg tcccggaccc gggaggcccc cacccccacg aacatctcca caaagtccga
     6241 cccactgatg gagaaaaaag gaacattggc ttccccggcg atggctcgcg ccaagagggt
     6301 tttacccgtt cctggcggac cgactagcag gactcctttg ggaatccgtc cgcccagacg
     6361 ggtaaacttt ttggggtccc ggagaaactc gataatctcc tgcagctctt ccttggattc
     6421 ttcgatcccg gccacatcct ggaaggtgac cttctgctgc ttgtcggaga gcagtttcac
     6481 cttggctttt ccgaaggaca tggccttggt tcccccagct tgcatttggc gcatgaaaaa
     6541 gatccaaaag gcgatcagg
//
LOCUS       DASPRR010000478         6564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_434334, whole genome shotgun sequence.
ACCESSION   DASPRR010000478 DASPRR010000000
VERSION     DASPRR010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434334"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /gene="msrB"
                     /locus_tag="VEL68_17575"
     CDS             <1..322
                     /gene="msrB"
                     /locus_tag="VEL68_17575"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXZ37837.1"
                     /translation="SGEPLFSSLDKFESGTGWPSFTKPLEPGNIVEKEDHGFFMTRTE
                     VKSKYGHSHLGHVFPDGPAPTGLRYCMNSASLRFIPKENLGKEGYGEYVKLFQKQEKV
                     GQAH"
     gene            complement(500..1303)
                     /locus_tag="VEL68_17580"
     CDS             complement(500..1303)
                     /locus_tag="VEL68_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37838.1"
                     /translation="MYSSLLPNELIKTRAFIYKNWTMAKRNVFTLFEILFWPVVGFFS
                     VGLLTEFAGMQPQMKAFVLVGVVSMGTIQVCQLDVAYVLLYDVWSKAVKHGFIAPVGI
                     RHLLLGSLVVGMARGAAVFFLLMGASYAFFQFDFRVPGIGHLALFLFGLFLCASTVGI
                     LVCILVLIFGNRADVAAWSLVSLMLLVCGIYYPVSILPEWVVPVAALIPLTYMLEYFR
                     QFYGFQPIFSHPLAKGYMLLLLYLLLEISFMKVALRRAKKSGLLLRLSE"
     gene            complement(1278..2150)
                     /locus_tag="VEL68_17585"
     CDS             complement(1278..2150)
                     /locus_tag="VEL68_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37839.1"
                     /translation="MEALKGVNLTVPAGVFWGILGPNGAGKTTFLSILSNLLTPEEGE
                     VRILGRDVRTDSMELRRRINLSSGHANFLWSLTVRENLEYYSMLYGLSKTDRQKKLEE
                     LFHLFDLSPFARVRFEELSTGTKQKLSLAKALLNDPELLLLDEPTVGLDPDVARRIRE
                     LVQKLHREKGTTILMTTHNMKEAEALCEEVAFIRGGMIKARGRPQDLKRDLRLGDTIV
                     VTFRGTLPSSSLEGLDGIYGLQCSDSSCRILVDDHRERLSQILDRLTGAKVAIHDLRI
                     QESDLEDVFIAFAQ"
     gene            2342..3631
                     /gene="eno"
                     /locus_tag="VEL68_17590"
     CDS             2342..3631
                     /gene="eno"
                     /locus_tag="VEL68_17590"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXZ37840.1"
                     /translation="MAKSDAIAKIRAREVLNFRGNPTVEAEVFLQDGSYGKAAVPAGI
                     STGRSEAVQVLDGDPKRFDGKGALRAGENVRKVIAPALKGMKATRQKEIDEKLIELDG
                     TPDKSKLGANAILAVSLATAYAAADYKNLSLFRYLGGKGPFRLPVPVYDMLAGGSHAQ
                     STVDLQEYLVIPCGVPAFQDSIQAGFNIYRALREVLQAKGHKITQVGGPLAPSLKSNR
                     EGVEVVAEAVERAGYKLGEEVFIGIDAATSELYEGGKYVLKGEGRSLTSKEMIDLWAE
                     WVASYPIVSIEDPLAEEDWEGWQEVTKRIGSQVQLVGDDLFATNPARIRKGIELKAAN
                     AVLIKPNQIGTLTETLEAIRITKKAGWGAMLSSRSGETEDTTICDLSVLDGAGQIKTG
                     PPNLQYIVKFNRLLRIAEEFGRRAKYAGHGAIKALKK"
     gene            3756..5225
                     /locus_tag="VEL68_17595"
     CDS             3756..5225
                     /locus_tag="VEL68_17595"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HXZ37841.1"
                     /translation="METRIQNMPTLLSTGNIPGRKAMVQILEAGLQAANPYHNARRLI
                     RVEGGKLVVGNPEFEPTGSPRTGDEVYDLSRVRNIYVVGAGKGIQNVARAIEEVLGDR
                     LTGGHVIDKKGHPIILKKIGVTLGGHPAPDEDCLRGCEKILEFAKGLTDQDLVFTCVA
                     NGVSSLLTLPAHDLTLEDLRQTTLVTQIERGMTTRELSPIRNHLDRMKGGRVSRYFHP
                     AKMIHILAIDPGDYEQRMYHNYWLHTLPDSTTFQDAVESLRRYDAWDAIPEAVRRHLE
                     RADPGEETLKARDFARMSFRIFGLMPGDWQSGKLLPAMKKAEELGFKAFLLADQLIEI
                     EASQAAIYMASMANTIERSGRPFEPPCVLFSSGELVVTVGKEKGIGGRNQEFALSAAL
                     RIEGSERIVIASVDTDGTDGPGFQFSHGEKEIPCLSGGIVDGWTGDEAKRKGVDIIGE
                     LKHHNTTPALWKLNSGVVVSPNISVIDLTAALILGPSKK"
     gene            5550..5897
                     /locus_tag="VEL68_17600"
     CDS             5550..5897
                     /locus_tag="VEL68_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37842.1"
                     /translation="MKKFWLLGMMALPLAFWALMASGAMAQDESLYKGHESEEMGPAT
                     SSHEYEGEEMTPAVPGQDQWNDEEMTPSGSLPKEGSEEMTPAGSGFKEEEYDSFRFAP
                     YRIPANSQPGSPY"
     gene            6219..>6564
                     /locus_tag="VEL68_17605"
     CDS             6219..>6564
                     /locus_tag="VEL68_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37843.1"
                     /translation="MGRRFFVSIFVFFLLLGGLREGEGEEKESFYSKSNRLIIRLEHV
                     EKAAAEGPGKVKEQNKSDGTAFFVQWADELYVVTARHVTEKGYDLRATVRIKDLRTGA
                     IKVAFLNLPRKHW"
BASE COUNT         1640 a         1649 c         1907 g         1368 t
ORIGIN      
        1 ctccggagaa cctctcttca gttcgctgga caagtttgaa tccgggacgg ggtggcccag
       61 cttcacaaaa ccccttgagc cgggaaacat cgtggagaaa gaggaccacg gttttttcat
      121 gacccgcact gaagtgaaga gcaaatacgg gcattcccat ctcggccatg tatttcccga
      181 cggaccggcg ccgaccgggc tccggtattg catgaactcc gcttccctcc gtttcatccc
      241 taaggaaaac ttgggaaagg aagggtacgg ggagtacgtg aagctgttcc agaagcagga
      301 gaaagtcggg caagcccact gagctgaagt ctgaggaatc aacccccccg ctcttctctg
      361 accgagaggg gcggggtttt tttatcgccc aataagaata ttacaggcgg cacattttta
      421 acggcaccat gcatcgtgcc cctacgggta agggaaatcc atttttatct acatggcggt
      481 agaagtaggg ccataagcgc tattccgaaa gcctcaaaag caggccgctc ttcttggccc
      541 ttctaagggc cactttcatg aaggatattt ccaggaggag atacaagagg agcagcatgt
      601 atcctttagc caggggatgg gaaaagatgg gctggaagcc gtagaactgg cggaagtatt
      661 cgagcatata ggtcaggggg atgagcgctg ccaccggaac gacccattcg gggaggattg
      721 aaaccgggta ataaattccg caaaccagga gcataaggct gaccagggac caggcggcca
      781 catcggcccg gtttccgaag atcagtacca gaatacatac caggattccc actgtggagg
      841 cgcagagaaa gagtccgaaa aggaagaggg ccaggtgccc gatccccggc acccggaaat
      901 cgaattggaa gaaggcgtaa ctggctccca tgagcagaaa gaagaccgct gccccccggg
      961 ccatccccac gacgagagag cccaggagga ggtggcggat tccgaccggg gcgataaacc
     1021 cgtgcttgac cgctttgctc cacacgtcgt acaggagcac ataggccaca tcaagctggc
     1081 agacctggat ggtgcccatg gagacgacgc ccacgaggac gaaggccttc atctgcggct
     1141 gcatgcccgc aaactcggtg aggaggccca cggaaaagaa gccgaccacc ggccagaaga
     1201 ggatttcgaa cagagtgaaa acattcctct tggccatggt ccagttcttg taaataaatg
     1261 cccgggtctt gatcaactca ttgggcaaaa gcgatgaata catcttcaag gtccgactcc
     1321 tggatgcgaa ggtcatgaat ggccactttg gcgccggtca accgatcgag aatttgggaa
     1381 agccgttccc ggtggtcatc caccaggatc cggcaggaag agtcgctgca ttgcaggcca
     1441 tagatcccgt ccaaaccctc cagggaggaa gagggcaagg tcccgcgaaa ggtgactacg
     1501 atcgtatctc ccaggcgcag gtcccgcttc aagtcctggg gccttccccg ggccttgatc
     1561 attcctcccc ggataaaggc cacctcttcg cagagagctt cggcctcctt catgttatgg
     1621 gtggtcatta aaatggtcgt gcctttttcg cggtgcagct tctggaccag ctcgcggatg
     1681 cggcgggcca cgtccgggtc aaggcccacc gtgggctcgt ccaggagaag aagttcagga
     1741 tcattcaaaa gggctttggc cagagatagc ttttgtttgg ttccggtgga gagctcttca
     1801 aaccgcaccc gggcaaaggg agaaaggtcg aaaagatgga agagctcttc cagtttcttt
     1861 tgccgatcgg ttttggagag accgtagagc atggagtagt actccaggtt ctccctgacg
     1921 gttaaactcc agagaaagtt ggcatggccg gaggaaagat tgatcctcct gcggagttcc
     1981 atggagtcag tgcggacatc cctgcccagg atccgaacct ctccttcctc gggggtcagg
     2041 aggttggaga ggatggaaag gaaagtggtt ttgcccgcac cgttcgggcc caggatcccc
     2101 caaaagactc cggccgggac ggtgaggttc acacccttca gggcttccac ccgccttttc
     2161 cggaaccatc cggaaacaaa agttttccgc agccctttga cttcgacagc gaaattcaga
     2221 tgcgcacctt tccttttgaa tttgtaagcc aatttatagt aacatcattt aggttcgttt
     2281 ttgaaaaatt tatcgatctt atcaggcgtc gaaaatacta ttttccctaa ggagaccggc
     2341 agtggctaaa tcagatgcaa tcgcaaaaat tcgggcgcgg gaggtgctga acttccgggg
     2401 caaccccacg gtggaagctg aggtttttct gcaagacggc agctacggga aagctgccgt
     2461 gcctgcgggg atcagcaccg ggcgcagcga ggcggtgcaa gtgctggacg gcgaccccaa
     2521 gcgctttgat ggcaaggggg ctctccgtgc aggggagaat gttcgcaagg tgattgcgcc
     2581 ggcactgaaa gggatgaagg ccacccggca gaaggaaatc gatgaaaagc ttatcgagtt
     2641 ggatggaacc cccgacaaga gcaagctagg ggccaacgcg atcttggcgg tctccctggc
     2701 tacagcttat gccgcggcgg actacaagaa tctttccttg ttccgttatc tgggaggaaa
     2761 aggccctttc cgtctgcccg tgccagtcta tgacatgctt gccgggggat cccatgccca
     2821 gagcacggtg gacttgcagg aatacctggt gataccttgc ggggtccccg cttttcagga
     2881 ttcgatccag gcgggattca atatttaccg agccttgaga gaagtgcttc aggcgaaggg
     2941 ccacaaaatc acgcaagtgg gaggccctct ggccccgtct ctgaagtcca accgggaggg
     3001 ggtggaggtc gtcgccgaag ctgtcgaaag ggcgggctac aagctcggcg aagaagtgtt
     3061 tatcggaatc gacgccgcca cttccgagct ttatgaaggc gggaagtatg ttttaaaggg
     3121 cgaaggccga tccctcacct ccaaggaaat gatagacctg tgggcggaat gggtagcgtc
     3181 ttatcccatc gtgagcatcg aagaccccct ggccgaggaa gattgggaag gctggcagga
     3241 ggtgacgaag aggattggaa gccaagttca actggtcgga gacgacctct tcgccaccaa
     3301 cccggcccgc atccgcaagg ggatcgagtt gaaagcggcc aacgccgtgc tcatcaaacc
     3361 aaaccagatc ggaaccttga ccgaaacact cgaagccatt cggattacta aaaaggcggg
     3421 atggggagcc atgcttagct cccgttcagg ggagacggaa gatacgacta tttgcgatct
     3481 ttccgtactg gacggcgccg gacagatcaa gaccggccct cccaacctgc aatacatcgt
     3541 caagttcaac cgcctgcttc gcattgcgga agagttcgga agaagggcga aatacgcagg
     3601 gcacggagcg attaaagcct taaaaaaata gtttcgggtt ccgagtttcg ggtttcaggt
     3661 ttaaaggcca aaattgcttt ggagaggtag ctgctcttaa gctaaaactc gagacccgag
     3721 acccgaaact tgttgctttg gagttttgcg tattcatgga aaccagaatc caaaacatgc
     3781 ccactcttct ttccacggga aatatccccg gccggaaggc catggtccag attctggaag
     3841 cgggcctgca ggccgcgaac ccttaccaca acgcccgcag gctcatccgt gtcgaaggcg
     3901 gaaaactggt tgtcggcaac ccggaatttg aacccaccgg gtctccccga accggcgacg
     3961 aggtctatga cttatccaga gtccgaaata tctacgtggt aggagccggc aagggtatcc
     4021 agaatgtggc cagggccatc gaggaagtgt tgggggatcg gcttaccggc gggcacgtga
     4081 tcgacaagaa gggccatccc ataatattga aaaaaatcgg tgttaccctg ggagggcatc
     4141 ctgcgccgga tgaagattgt cttcgggggt gcgagaaaat tctggagttc gccaaaggcc
     4201 taaccgatca ggacctggtt ttcacctgcg tggccaacgg cgtctcttct cttctgacct
     4261 tgcccgccca tgatttaacc ctggaggacc ttcgccagac cacattggtc acccagatcg
     4321 agcgggggat gactacgaga gaactgagcc cgatccggaa tcacctggac cggatgaaag
     4381 gagggcgggt ctcccgctat ttccatccgg caaagatgat ccatattctg gccatcgatc
     4441 ccggcgatta tgaacagcgg atgtaccaca attactggct ccataccctg ccggacagca
     4501 cgaccttcca ggacgccgtt gagagtctga gaagatacga tgcctgggat gctatccccg
     4561 aagcggtccg cagacatctg gaaagagcgg acccggggga ggagactttg aaagccaggg
     4621 actttgcaag gatgtctttc cgcattttcg gcctcatgcc cggagactgg cagagcggga
     4681 agttactacc ggccatgaag aaggctgagg aactgggatt caaagctttt ttactggccg
     4741 atcaactgat agagatcgaa gccagccagg ctgccattta catggcttcc atggctaaca
     4801 ccatcgaaag aagcggtcgg cccttcgagc ctccctgtgt ccttttctcc agcggggagc
     4861 tagtcgtgac cgtaggaaag gaaaagggga tcggagggag aaaccaggaa ttcgccctct
     4921 cagcggctct aaggatcgaa gggagcgaaa gaatcgtcat cgcctcggtg gatacggatg
     4981 gtaccgacgg acccggcttt cagttttccc acggggaaaa agaaattccg tgcctttccg
     5041 gagggattgt ggatggctgg acaggagatg aagccaagag gaaaggtgtg gatataatcg
     5101 gagaattgaa acaccacaat acaactcctg ccttgtggaa attgaacagc ggggtcgtgg
     5161 tttccccaaa cataagcgtc atagatttaa ccgcggcttt gatcttggga ccaagcaaga
     5221 aataaatttc gaattccggg tttcaagttt aaggtcctgc ccacccacaa taccttcctt
     5281 cccgggtcat tttaataacc cggcgaaagt tattgggttt tattcctttt cttccagcca
     5341 gttgggtcag caactatttc tggaacctcc cggggtcttt gctcatctaa acaagcaaga
     5401 aagcagtatt tcctgccaaa gggattcggc aaaggaccag gcggagcaga aagcgcagtt
     5461 cggaatgggt gatcagacaa ttaggttatt tgggtgtaat gaaggtagat agaagcgaga
     5521 gagttcattg accaaagggg gtgagtagaa tgaaaaaatt ctggctattg ggaatgatgg
     5581 ctcttccgct ggccttttgg gctcttatgg cttcgggagc catggcgcaa gatgagtcgc
     5641 tatacaaagg acacgaatcc gaagagatgg gccccgcaac ttcttcgcat gaatacgagg
     5701 gcgaggagat gactccagcg gttccaggcc aagaccaatg gaacgatgag gagatgaccc
     5761 cttccggttc cttacccaag gaagggagtg aagagatgac cccggcgggt tcgggattca
     5821 aagaagagga gtatgacagt tttagatttg ctccctaccg gattccggca aacagccaac
     5881 ctggttcacc ttattaagaa tagggttggt tctctgggcg gacaagagga agcacacccg
     5941 agttgggtgt gcttcttttt tttttcgagg ctcctttttt gcggggggac atttgaagtt
     6001 ttcacggata gtgctaatat ctcattaaat ggctggggaa actccctggg gcacggtcac
     6061 taccgggaac ccacgaaatc cgtagaggtg ttctgttctc gctggagtca tcgccagaaa
     6121 ggaaagggtt ttgttggcaa actgctaaag ttcaacgaag ggtattcccg atttcttcgg
     6181 cgagttctta atcataatcg cagcggagaa aaatgcagat gggaagaaga ttttttgtat
     6241 ctatattcgt atttttttta ttgctgggag gcctcaggga aggagaaggc gaggagaagg
     6301 agagttttta ctccaaatcc aaccggctta tcatcaggtt agagcacgtc gaaaaagctg
     6361 ccgccgaagg ccccggcaag gttaaagagc aaaacaaatc cgatggaacg gccttcttcg
     6421 tccagtgggc ggatgagttg tatgtagtca ccgccaggca tgtgactgaa aagggatacg
     6481 atctgcgtgc cacggtccgg attaaggacc tcagaaccgg ggccatcaag gtggcttttc
     6541 tgaaccttcc ccggaaacat tggg
//
LOCUS       DASPRR010000479         4904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2210407, whole genome shotgun sequence.
ACCESSION   DASPRR010000479 DASPRR010000000
VERSION     DASPRR010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210407"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VEL68_17610"
     CDS             complement(<1..421)
                     /locus_tag="VEL68_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXZ37844.1"
                     /translation="MELAGKRIAILVEDMYQEHEFWYPFYRMKEAGAKVTVVGTGAKE
                     YHSKIGLPAPGGVPAESVGAPDFDAVIVPGGFAPDRMRRNAAMLKLVRDCFTQGKVVA
                     AICHAGWVLVSAGILKGKKATCVSAIKDDVVNAGATYV"
     gene            626..1762
                     /locus_tag="VEL68_17615"
     CDS             626..1762
                     /locus_tag="VEL68_17615"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HXZ37845.1"
                     /translation="MGINKKIMGQKELQKKVLETGLCTHCGACVNLCPYAVSYKDHTI
                     LLDPCGREEGRCYAFCPRTPTDLQTLRQRYDPHDLTPELGAVRGFHLTRAAEDKVRQS
                     SQHGGTVTTLVSLALEEGIIDTAILAEEGAGFLPEGIAVHRVSDVKKRGKSRFVVSPN
                     LAAFHKAAKSSSQKIGVVATPCQAVALAKMQQKPYPSKDSNIDKLRLVIGLFCGWALS
                     WRELKAFLEKRFGNNSILGLDIPPSKHNSMEIHTPGGIVEIPLDEVIPFVRKSCGYCW
                     DMTAEFSDLSVGSARLPEGWAVARGWNQVIVRTAAGQELLELARSRKVLEFREMPEGN
                     LERLKKASLNKKRAALQNLAQMSGNPEDLLYLDSQDPVLRTLIS"
     gene            1984..3897
                     /locus_tag="VEL68_17620"
     CDS             1984..3897
                     /locus_tag="VEL68_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ37846.1"
                     /translation="MSVITLNEPGKELLMMGNEAIARGALEAGVKVGAAYPGTPSTDI
                     IENLASVASQMGLYVEWSINEKVALEVAAAASFSGLRAICAMKQNGMNVASDYLMTLN
                     LVGVKTGLVLVACDDPQGHSSNNEQDSRLFAKMADLPLLEPSTFQESKEMTKWAFELS
                     EQIGNVVILRGVTRTSHARGNVVLGQLPKTEVKPRFDRSKQWLTQPLVPKHQQLHRNL
                     DRLEEIYAESPFNQYLGPGSPKVLIITSGTGWLYSQEAVEILGLEGSVGILKVGTTWP
                     LPTQWIVENLKRTDQVLLIEEVDSFLEGNVKEIAADHSRKIGPKDFFGKKSGHVPPMG
                     EMTPDRVVDALAKIFRVPYQPRTSEYETRAQEVAKTMLPSRALGFCAGCPHRASFWSI
                     KNALQLDNRQGFATYDIGCYALARGPAGYYQLRVGGAMGTGTGLASGFGRLSALGFEQ
                     PVIAMCGDSTFFHSAMPALVNAHYNKSNLVMIILDNSATAMTGFQPHPGVGRNAMGEM
                     VSPVDIEGVCRAFGAKVEIADPFDIPDTRNKILRALEDREGAKVLILRRRCQMLKGKD
                     EKPPYRVWVDPQKCIGEDCGCDRVCTRVFKCPGLIWDAKEGKSKLDEVICIGCGVCAE
                     ICPEGAIIKEETK"
     gene            3898..4509
                     /locus_tag="VEL68_17625"
     CDS             3898..4509
                     /locus_tag="VEL68_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXZ37847.1"
                     /translation="MASKKETYNLVITGVGGQGNVLSSQLIGQALVGKGYFVTIGETY
                     GASQRGGSVMSHIRISSEKQLSPLIPRGKADIVIGLEPVEALRVLTVYGNSQTVVLSN
                     TRPIYPVDVTSGDEKYPENEEIQQALKDLSQKVHYIPATEKAMEMGSSILGNMIMIGA
                     LLELDLLPLSPAEFRKTLGRNFKEARLETNLRALEEGKRAVRP"
     gene            4546..>4904
                     /locus_tag="VEL68_17630"
     CDS             4546..>4904
                     /locus_tag="VEL68_17630"
                     /inference="COORDINATES: protein motif:HMM:NF041086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAUR_1299 family protein"
                     /protein_id="HXZ37848.1"
                     /translation="MDPFRDLLFSKAFQVVPGSRYNQVSQDLRETWGILFYEVVLRED
                     TPWEHLRDQIYPVLARYLRYKSISPELPKGVVVSLFFHDRFYLLQASDFLLAYQEKEE
                     LDPDAFHARVLEWLAKP"
BASE COUNT         1132 a         1408 c         1389 g          975 t
ORIGIN      
        1 ccacataggt ggccccggca ttcactacgt cgtccttgat cgccgaaacg caggtggcct
       61 tcttgccctt gagaatgccc gcggaaacga gcacccaccc cgcatggcag atggccgcca
      121 cgactttccc ctgggtgaaa cagtcgcgaa ccagcttgag catggccgcg ttccggcgca
      181 tgcggtcggg cgcgaatccg ccgggaacga tcaccgcatc aaagtccggg gcgccgaccg
      241 attctgccgg gactcccccg ggcgccggca gtccgatctt gctatgatac tccttggctc
      301 cggtgccgac cacggtgacc ttcgcccccg cctctttcat ccggtagaag ggataccaga
      361 actcatgctc ctggtacatg tcttcgacca aaatggcaat ccgttttcct gccagctcca
      421 taaaaacctc ctttgtattt ctaatcctta tatttaatta taacaacccc atctgaggtc
      481 accaagagaa ttagtgaaga attttaactt gaaacccgaa acctaagaat tgtttttatg
      541 cctttgctcc ttgacttccc gccatttcct gggatatgaa ataaagagaa ggcgattatc
      601 gcaaggaagg acttgggtct taccgatggg aataaacaag aaaatcatgg ggcagaagga
      661 acttcagaaa aaggttctgg aaacgggtct ttgcactcat tgcggagcct gtgtcaacct
      721 ttgtccttac gcagtctcgt acaaggatca caccatcctc ctcgacccct gcggacggga
      781 agaagggcgc tgctatgcct tctgtccccg gacgccgacg gacctccaga ctttgcgcca
      841 aaggtatgac ccccatgacc tcaccccgga acttggggcg gtccgggggt tccacctcac
      901 ccgggcggcg gaagataagg tccgccaatc atcccagcac ggcgggacgg ttaccactct
      961 tgtatccctc gccctggagg aagggatcat cgacaccgca atcctcgccg aagaaggggc
     1021 gggcttcttg cccgagggga tcgccgtcca tagagtctcc gacgtaaaga agagggggaa
     1081 gagccggttc gtggtatctc caaacctggc tgccttccat aaggcggcaa aaagctcgtc
     1141 ccagaaaatc ggagtggtgg ctaccccttg ccaggccgtg gccctggcca aaatgcagca
     1201 gaaaccctat ccatccaaag acagcaacat cgacaagctt cggctggtaa tcgggttatt
     1261 ctgcggctgg gccctctcct ggcgggagct gaaagccttt ttggagaaaa gattcgggaa
     1321 caactccatt ctcgggctag acattccccc cagcaaacac aattccatgg aaattcatac
     1381 tcccggggga atcgtggaga ttccgctgga tgaggtgatc ccctttgtcc ggaaatcctg
     1441 cggttactgt tgggacatga ccgccgaatt cagcgatctg tccgtgggat cggcccgtct
     1501 cccggaagga tgggcagtag cccggggatg gaatcaggtg attgtcagga cggcggcagg
     1561 acaggaatta ttggagctgg cccgttcccg gaaagttctg gaattccgag agatgccgga
     1621 aggaaatctg gaaaggctga aaaaggcttc tctgaacaag aaacgggccg cccttcagaa
     1681 cctggcccag atgagcggga atcccgagga cctcctgtac ttagatagtc aggatccggt
     1741 cctgagaacc ttaatcagct gatgagagcc caaaccccat tttactctcc cttccaggga
     1801 gactgtgtcg taatgggaaa aacgccgtca ttcccgcgaa agcggaaagc caggtccttg
     1861 agattattct tttctccaac taactggatg tccgcctccg agggcatgac gaattacgat
     1921 acagtcgcaa gggagagaag gcaaggtgaa gggaaataat cagcctttga aagaaaggtg
     1981 atcatgtctg tcattaccct gaatgaaccg ggaaaagaac tcctcatgat gggcaacgaa
     2041 gccatcgccc ggggggctct ggaagccgga gtcaaggtgg gggccgccta cccgggaacc
     2101 ccttccaccg atatcatcga gaacctggcc agcgtagcct cgcagatggg actctatgtg
     2161 gagtggtcca tcaatgaaaa ggtggccctg gaagtggctg ccgcagcctc cttctccggg
     2221 cttcgggcga tctgcgccat gaagcagaac gggatgaatg tggcctccga ctacctgatg
     2281 accctaaacc tcgtgggagt caaaacgggc ctcgtcctgg tggcctgcga cgatccccag
     2341 ggccattcca gcaacaacga gcaggactcc cggctcttcg ccaagatggc cgatctcccc
     2401 ctcctggagc cttccacctt ccaggaatcc aaggagatga ccaagtgggc cttcgagctc
     2461 tccgagcaga ttggcaacgt ggtcatcctg cgcggggtca cccgaacctc ccatgcacgc
     2521 gggaacgtag ttttaggcca actccccaag accgaggtga aaccccgctt cgatcgttcc
     2581 aagcaatggc tcacccaacc cttggttccc aaacaccaac agctccaccg caacttggat
     2641 cgtctagaag agatttacgc ggaaagcccc ttcaaccagt acctgggtcc cgggtctccc
     2701 aaagtcctca tcatcaccag cggtaccgga tggctttaca gccaggaggc ggtggagatt
     2761 ctcgggctgg aaggctcagt ggggattctc aaggtcggga cgacctggcc ccttcccacc
     2821 caatggatcg tggagaattt gaagagaacc gaccaggtcc tgttgatcga agaagtggac
     2881 tctttcctgg aaggaaatgt caaagaaatt gcggcggacc attcccggaa gatcggaccc
     2941 aaagattttt tcgggaagaa atcgggacat gttccgccca tgggagaaat gacccctgac
     3001 cgggtcgtcg atgccctggc caagattttc agggtgccct accaacccag aacttccgag
     3061 tacgaaacgc gagcccagga agtggcaaag actatgcttc cctcccgggc cctggggttc
     3121 tgcgccggct gccctcaccg ggcctctttt tggtcgatca agaacgccct ccagctggac
     3181 aaccggcagg gatttgccac gtatgacatc ggatgttatg ccttggcgag gggcccggct
     3241 ggctactacc agttgagggt ggggggggcc atggggacgg gaacgggcct ggccagtggg
     3301 tttggaaggc tctccgcttt ggggttcgaa cagccggtga tcgccatgtg cggcgattcc
     3361 accttcttcc attcagccat gcccgctctg gtcaatgccc actacaacaa gtccaacctc
     3421 gtgatgataa tcctggataa cagcgccacg gccatgaccg ggttccagcc ccatcccggc
     3481 gtgggaagga acgccatggg agagatggtt tctccggtgg acatcgaagg ggtttgccgg
     3541 gccttcgggg caaaggtaga aattgccgat ccttttgata ttcccgacac aagaaacaag
     3601 atcctccgcg ccctggaaga ccgcgaggga gccaaagttc ttatcctgcg ccgtagatgc
     3661 cagatgctca agggaaagga cgaaaaacct ccctaccggg tctgggtgga cccccaaaaa
     3721 tgcatcggtg aagactgcgg ctgcgaccgg gtgtgcaccc gggtcttcaa gtgccccggg
     3781 ctgatctggg acgcaaaaga ggggaagtcc aagttggacg aagtgatctg catcgggtgc
     3841 ggggtgtgcg ccgaaatctg cccggaaggg gccatcatca aggaggagac gaagtaattg
     3901 gccagcaaga aagaaaccta taatctggtc atcaccggag ttggcgggca ggggaacgtt
     3961 ctctcctccc agctcatcgg ccaggccctg gtgggcaagg ggtattttgt gaccatcggg
     4021 gagacctacg gcgcctccca gaggggaggc tcggtgatga gtcacatccg catctcctcc
     4081 gagaagcagc tcagccccct tattcccagg gggaaagcgg acatcgtcat tgggttggag
     4141 ccggtggagg cccttcgggt tctgacggtt tacgggaatt cccaaaccgt agtcctttcc
     4201 aatacccggc ccatctatcc cgtggacgtg acctcagggg acgaaaagta tccggagaac
     4261 gaagagatcc aacaggccct gaaggacctg tcccagaaag ttcactatat tccggccacg
     4321 gaaaaagcca tggagatggg ttcgtccatc ctgggcaaca tgatcatgat aggggccctt
     4381 ctggaactgg atctcctgcc gctcagcccg gccgagttcc ggaagaccct gggcaggaat
     4441 ttcaaagagg caagactgga gaccaacctg cgggcgctgg aagaaggaaa gcgggctgtt
     4501 cgtccttaaa gaagcggttg ggctcttttc cgaggccgga aaaaaatgga cccatttcgg
     4561 gacctcctct tctccaaagc cttccaagtt gttccagggt cccggtacaa ccaagtttcc
     4621 caggacctcc gggagacgtg gggtatccta ttctatgaag tcgtcctccg ggaggacacc
     4681 ccctgggaac acctgcgaga ccagatttat cccgtcctgg cacgctatct aagatacaaa
     4741 tccataagcc ctgagctgcc aaaaggagtg gttgtctccc tgttcttcca tgaccggttt
     4801 taccttctcc aggcctcgga cttcctgctg gcttaccagg aaaaggagga gctggacccg
     4861 gatgcctttc atgcccgggt actggagtgg ctcgctaagc ccga
//
LOCUS       DASPRR010000480        15257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1566696, whole genome shotgun sequence.
ACCESSION   DASPRR010000480 DASPRR010000000
VERSION     DASPRR010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15257)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15257
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566696"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            599..1783
                     /locus_tag="VEL68_17635"
     CDS             599..1783
                     /locus_tag="VEL68_17635"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37849.1"
                     /translation="MMTKLRKASKMRVGILFGILLAFGVSTVFYGQTGFAQSSRLKDY
                     KFGMILALTGAGAWYGVTMGRGAELAMEEINGAGGASGYKLISVIEDHRSGDTSAGQA
                     SVRKLIDIDKVGFIAGSYGSVCTSIQPICAESHILLINGGGTAPSLLNKPYLYNTRAL
                     GNTCAIGVLKYLWDKKGSRKLATIYYNQESGVTINQAAVKEWKSWGGTVVKETMVATG
                     QTSFTGEISQIKAAGPDCIGIWSYGTDVGYTVKDIKRTGITAPVCGVEFTPDALAIAG
                     KSFEGFLIGLDFFDPKLSNPMTEKFVKHFYAKYGADAKLDYYCANYYDITYLLRDLIS
                     RVAAKGGNPFDGAQLLAALQQNPRFDTVYGGKMELNKDGSVNKPISIFEVKEGKQVRI
                     SQ"
     gene            1824..2699
                     /locus_tag="VEL68_17640"
     CDS             1824..2699
                     /locus_tag="VEL68_17640"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37850.1"
                     /translation="MLQLVVAGIIWGSLYALVGVSWGLIYSTTRTFHFAHGLTFTLAA
                     YGVILSHGFFHLPLFVSFGVGLLVAVSCGCAIEFLVYRPVRRRSSGQLGIFLSAMGTL
                     VLGESLIQIFFTPTPRTLEIPDISDIPFSYGTIFFTLADLLLVLVAWGIISILELYLS
                     RSKVGREIRAVRSNPELATSVGIRTEKAFLITFAIGSMLMGLGSFFVTIQSAATPHMG
                     LNYVLMAFIATFLGGVGKDWGIALAGLAMGLVENLPLVVLPAHYKYIVVFAMMFGFLL
                     MKPEGLLGARNRVRR"
     gene            2720..4438
                     /locus_tag="VEL68_17645"
     CDS             2720..4438
                     /locus_tag="VEL68_17645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXZ37851.1"
                     /translation="MDYLAHIINMVLIFTMLASSLNLILGYGGMATMCHAALFGIGGY
                     ASAILTMNLGCNFLVGTILAALLSGFIGVLLASPSLRIQDEYLILFTTAFQMVIWGLM
                     VSEVWITNGETGLSAIPRASVFGIQFLKPISYIPLIAALAALFFLICWKVSHSPFGRV
                     LRCIREDELSTRSIGKNVLRFKVLTFMVGGAIAGGAGSILAHYNAYINPVSFNLDASI
                     LMIAMVVLGGSTNMFGSVVGSLLLVGIPEMLRFVPGTATLIAPLRNAIFGSLLIFFIL
                     FRPQGLIPEHVGRLRVRRTPLPKLPEEEILTLSEQEGPAKSQGVSEPVIEVKGIRKSF
                     GGIQAVNSFSMSLSPGKITTLIGPNGCGKTTVFNLISGFISPDEGSIYLRGKDVTNRQ
                     PHELVQLGLVRSWQDVRIFRNMSVLDNVMAAIPDQIGERLFAIFFRPRRVRQEEWKNT
                     RMALAYLEFVGLAEKAYELAGELSFAEQKQLSLARLIATRGSILLFDEPASGMDVVST
                     KNIQKMILRLAGFGKTICVVEHNLDLVKELSDESLFMNQGDVIRKAKPEELMTDPQLA
                     LVYFGG"
     gene            4453..5145
                     /locus_tag="VEL68_17650"
     CDS             4453..5145
                     /locus_tag="VEL68_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37852.1"
                     /translation="MEMKAVSAGYYRKRVIHDISLSVDRSEVVSLIGHNGAGKTTTLK
                     TILGVIKSEQGEIRFRDRRITNQDPVENVTAGIRFIPQERFTFPDLTVWENIMLGAHL
                     VQQKEKIGKSIDEVYNQFPILKKRLSQKAGTMSGGEQRMLSMAMAMMAQPQFVMVDEP
                     SLGLAPLIIQEIGRIIGRMGDQGMAVLLVDQNIKQTLKISNRVYVMKNGQIVLEETSQ
                     KLLDRGEWWDLY"
     gene            5251..6399
                     /locus_tag="VEL68_17655"
     CDS             5251..6399
                     /locus_tag="VEL68_17655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37853.1"
                     /translation="MRKSIGVGAGTVLLGAALLMIVPTAVLGQTKAPVKIGVILPMTG
                     PAALYGEQANRGVQLAAKMVNGAGGILGGRQVSIILEDDQANPVEGVNAVKKLMNRDG
                     TKLITGGVNSSVSLAELQVTKEAGLLHIVTVSTAPAIREQGHPSLFALQPTNSMKDDF
                     ALPWIAQVLKPKTVVHLLENTDYGRVEVEELKKHWGASGPKVVATETFQLGETDFTIH
                     LNKFKAMKPDLFYLSTAAPSTIGTILKQAYEIGFKAPIFLAPGNLNNDVVRLAGPAAE
                     GVLCADYYVNILDTPANKKFVKDHEEMFKRPPEKMEMLGFESITILSKALDMAGTETD
                     VKKISDTLRQNKWQTPRGEIVFDAKGQAKGVGKDQALVVKDGKITIRK"
     gene            6449..7381
                     /locus_tag="VEL68_17660"
     CDS             6449..7381
                     /locus_tag="VEL68_17660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37854.1"
                     /translation="MDPRQNGVRSMSLPTIGSTSMIDLDLLVIQLANGLVIGMGYTLV
                     AIGLTMVFGILGVLNFAHGEFFMLGAFVAYVFGELMGWPYVVSSVVAIVLVMAISFLF
                     ELISVRPFMERDPEMTLLSTFAASLILMNLVEVIWGNTPLPLNTPFSKPLDLLFLVLT
                     AQRILILLVGAVGVILLGLFLKFTKMGKVIRATAQNKKASALVGINIKKVYRLTFAAG
                     TGLAAIAGVLIAPLSNLYASMGQGMVLKGFVVVILGGLASVPGAILGGLTLGITEAIA
                     AGYVSSAWKDVIGFSILIIILLLRPQGLMGRREA"
     gene            7378..8373
                     /locus_tag="VEL68_17665"
     CDS             7378..8373
                     /locus_tag="VEL68_17665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37855.1"
                     /translation="MNRAARNRRNWTRFLGLVVALLLPIVFQDAYLRNILVIFGIYCL
                     LALSLNIIVGYIGEVSLGHAAFFGLGGYASAILSKTVGFPVLLSIPSAVVISGILGLF
                     IGYLALRLRGPYFAITTLAFAEIIRLIVTNWVDLTRGPLGFTQIPPLVLGIPGWFEFK
                     VMSELRYYYFVLVLVGLVFLLIYNVVHSPTGRTFLAIREQEPLARSVGISPFRYRVLA
                     FVISTMIAGLAGAAYAHFFRIISPDINMVYYSAIPLMMVMVGGRGTLLGPFLGALLFA
                     VLPELFRTAGTIRMVFFASFLLATIVYMPEGIAVKIQTFIRDWETKKSPQELGDV"
     gene            8388..9176
                     /locus_tag="VEL68_17670"
     CDS             8388..9176
                     /locus_tag="VEL68_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37856.1"
                     /translation="MPILEGKNLTRAFGGLMALREVSLEVQPHEILGIIGPNGAGKTT
                     CFNILTGFYSATSGTVFFEGQEVSSMPMEKRALLGMVRTFQHTSLFPKLTVYENVLTA
                     NYKMNATNLLHHIFRSGAFKKTERELDKKAMEVLEFLGLREQRDMMARNLPYGSQRKL
                     EIANALATDPKLLLLDEPAAGMDPVESQDLMRLIKEIQKRGITIMLVEHNMKLVMGIC
                     DRLVVLNYGSKIAEGKPKDIAQNPDVINVYLGKKQDSFRLKNRG"
     gene            9187..9894
                     /locus_tag="VEL68_17675"
     CDS             9187..9894
                     /locus_tag="VEL68_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37857.1"
                     /translation="MLKLESVRVFYGNVQALKGISIAVEDHELVALIGGNGAGKSTTL
                     LTVSGLTRPRSGEISYRRQRIDHLPPFKILEMGIAHVPEGREIFPELTVLENLEMGAY
                     RSPDEESSQGQKLKEMYGYFPVLEERKKQLGRTLSGGEQQMLAIARGLMSSPKLLLLD
                     EPSLGLAPLVVDRLAEIILQLHEKGLGILLVEQNAQMALGMADRGYVLETGQIVLQGP
                     GRDLLNDENVQKSYLGL"
     gene            9995..11224
                     /locus_tag="VEL68_17680"
     CDS             9995..11224
                     /locus_tag="VEL68_17680"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37858.1"
                     /translation="MKKAGFLFVCMLVLIGWALPGNAQKPAGKPILIGYISHLTGPAA
                     KVSQQEIAAAQATADMINEKGGVLGRPLELITRDDHTDAGVATRVARELILDKRVNFL
                     AGCSNTKSSLAISEVAKQQKVIYIIATSQTAAITEELGHRYVFRSWSNSYSQIGPAAL
                     WMAKGKFKRFVTSAGDYEWGHNSVDTILSLVKKHRTDFEVLKQVWPPYGTGDYSPYLS
                     QLMAVKPDLIIAAHYGSETTNFIRQAKALGLVGKPDSPQLIIYMDQDNKEALGKEMPE
                     GIGGYCAFHTRSINNPWADWFDNVVHKRTGSWPGGIALGGYMCVSWLAKGVEKAGTTD
                     TEAVVKALEGLEMETYVGKIKIRECDHQATGVQPLGFTKASSDYPFCVMTDLYYPPGE
                     QFLHTCKEIEALRAPKK"
     gene            11395..12279
                     /locus_tag="VEL68_17685"
     CDS             11395..12279
                     /locus_tag="VEL68_17685"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37859.1"
                     /translation="MVDLIITQILTGIGAAGILLLMAVGLSLVFGVLHILDFVHGSIF
                     LFGGYIGYTFSILLARVEGGFWLSLVAGGLMAAALGTLIEILFLRRVYDRQLLLQFLI
                     LFGVVYIVSDVMKIFWGTGYLSISRPDFLSDSMSYAGGRVVVARYNIFVIVLGAATIA
                     LLWLLMNRTRFGLQVRALHQQKEMAALLGMNTPMLRTSVFAIGSGMAGLAGVAIGGLG
                     SLSPGMDVEAVILAFSIVTIGGLGSVKGAIVGALIVGLVQCLGLLILPQLNITFIYLV
                     MAVVLVVRPWGLFGRATA"
     gene            12332..13279
                     /locus_tag="VEL68_17690"
     CDS             12332..13279
                     /locus_tag="VEL68_17690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ37860.1"
                     /translation="MGGMSSKNGFTLVVILAALCLLPWVVSPMIILVLSEALIWVLFA
                     IATNIVLGYGGRITFGHAAFFGTGGYVCALLLKKLEAGLPAGIFCSAAVSTGMGLIIG
                     WFCVRLTHVYFAMLTMAFGQLVWAVVYRWRYAGADTGIIGVPGLGSPVEFYFLVLVLV
                     SLSVLVIYFIVHSPFGKVLVGTRESEVRPPFIGINVRRQQLVAFVISAFFSGISGGLF
                     VVLNGIAYPDILTWVYSGQVLVMCLLGGMTVLLGPAVGAVILIFLQHWITGYTQYWPL
                     VLGLIIVLLVLFLPNGILGSLLDRGRRGLELPVWLRPQK"
     gene            13455..14210
                     /locus_tag="VEL68_17695"
     CDS             13455..14210
                     /locus_tag="VEL68_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37861.1"
                     /translation="MALLEIQDVTKAFGGVQAVDHVTLSFEESGLTSVIGPNGAGKTT
                     LFNLITGHLTPSGGRIVFAGEDITRLPSFEIWYKGLCRSFQITSLFPSLTVFENIQLV
                     QLTRHRKQFNLFQRAAHLYQEETFEILKAVELADQANMTVSSMPYGDQRKLEIGIVLA
                     SNAKLMLLDEPTAGMARVERLAMAGFIERLFQERGLSIVFIEHDMDVVMSISQKIWVM
                     HQGRIIVEGTPDEIRRNDEVRRIYLGEQNLWSS"
     gene            14198..14902
                     /locus_tag="VEL68_17700"
     CDS             14198..14902
                     /locus_tag="VEL68_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37862.1"
                     /translation="MVLLEIEKINTFYGLSHILFDVSLKIEKGEVVCLLGRNGAGKTT
                     LMRSIMGLNPPRSGSILFQGEPIIGKPTFRISRAGIGMVPENRLIFADLTVRENLEAG
                     WRDRGENGWTVEKVWNLFPELKRLSSNRGGQLSGGEQQMLSIARTLLQNPALLLLDEP
                     TEGLSPLLVHRIADRVLEIKSGKVLTMLIAEQNYPIASKMGDRSYILEKGLIQFEGTM
                     TELRRNPEVTKKYLAI"
     gene            complement(14899..15102)
                     /locus_tag="VEL68_17705"
     CDS             complement(14899..15102)
                     /locus_tag="VEL68_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37863.1"
                     /translation="MALVCSLPASEPASGSVKANAGIISPEAIPGKNFLCILAEPAST
                     IGWLANPWVAMVAPARLYPLANS"
BASE COUNT         3380 a         3803 c         4374 g         3700 t
ORIGIN      
        1 ttatagaaaa aatccttgac atacaaccaa taaaaaatta ttttaaggca gacaaaggtt
       61 ccaggtctta actttttgta tacaataaga ttgcagacag aaccaaaatt gggtattccg
      121 gacctgcgtc cttgtatccc aagccagaga cgcgtataca ggttctcgca acggatgcaa
      181 agacttgatc ggctgtttta tttcatttga acggcttggg gtctagatta tgcctttaca
      241 tcggctaatg gccgattcca agaacactgc aatgtgcccc tcggcggttt ttccccggtc
      301 aaggccgcga atcaaagttc tgccaaatta agataatgca ctcaatgcga ttgtttgcaa
      361 tgcgggactg gggagatatt atttggctgt aatcagaatg ctttgttcct tgaagaaaat
      421 ggtctcgtca agagtcagaa aagggtgcca tcacgagcaa agcggtgcag cgtggttttt
      481 gaaaattccc gaaatataag aaaaccgcct ctgctccgac cgcgggaggc ttctcgcagt
      541 aaccaatggg ggattttatt gagctcatta aaggcggaca ataaacaagg aggggattat
      601 gatgaccaag ttaagaaaag ccagtaaaat gcgcgtaggt attctcttcg ggattcttct
      661 tgcctttggg gtttccacag ttttttacgg gcaaacgggg tttgcccaga gttccaggct
      721 aaaggattac aaattcggca tgatcctggc cttgacggga gcgggagcat ggtatggagt
      781 gacgatgggc cgcggagcgg aattagcgat ggaggaaatc aatggagcgg ggggggcaag
      841 tggatataaa cttatatcgg tcatagaaga ccaccgaagc ggagacactt ccgcaggcca
      901 ggccagcgtc cgcaagttga tcgatatcga taaagtaggg ttcatcgcgg gctcttacgg
      961 gagtgtatgc acttccattc agcccatatg cgccgagagc catatcctac tcattaatgg
     1021 cggcggaacg gcgcccagtc tgctgaacaa gccttatcta tacaacacgc gagcccttgg
     1081 caatacctgt gcaatcgggg tactgaagta tctttgggat aaaaagggga gccggaaact
     1141 ggcgactatt tattacaatc aggagtccgg ggttacgatc aaccaggcgg ctgtcaagga
     1201 gtggaaatcc tgggggggga cggtggtcaa ggaaacgatg gtcgcaacgg gccaaaccag
     1261 ctttacgggc gagatctccc aaattaaggc cgccgggcca gattgcatcg ggatctggtc
     1321 ttacggaacg gatgtcggtt ataccgtgaa ggacatcaag agaacgggga tcacggcgcc
     1381 ggtttgcggg gtggaattta ctccggatgc actcgccatc gccggaaaat ccttcgaggg
     1441 atttctgatc ggcttggatt tctttgaccc taaactgagt aacccgatga cggaaaaatt
     1501 cgtgaaacat ttttatgcca aatatggggc tgatgcaaaa ctggattact attgtgcgaa
     1561 ttattacgac atcacttacc tgctcaggga tttaatctcc cgggttgcgg caaaaggagg
     1621 caaccccttc gatggagctc aactcttagc ggccctgcag cagaatcctc gcttcgacac
     1681 ggtttacggc ggcaaaatgg aactgaacaa ggatggaagc gtcaacaaac caatctccat
     1741 ttttgaggtc aaggagggca aacaggtaag gatcagccag taaccaagga attgaatttg
     1801 ccaaatttta gggcaggtgg ggtatgctgc aattggttgt agccggcatc atctggggga
     1861 gcctttatgc cttagtggga gtgagctggg ggcttattta cagtaccacg cggactttcc
     1921 attttgctca tggtcttacg tttactctgg cggcctatgg ggtgatcctc tctcacggat
     1981 ttttccattt gccgttgttt gtttcgttcg gcgtggggtt actggtggcg gtatcgtgcg
     2041 gatgcgccat cgaatttctg gtttaccggc cggttcggcg gagaagctcc gggcagctag
     2101 ggatcttcct gtccgccatg ggcaccttgg ttctgggtga atccttgatt caaatttttt
     2161 tcacccccac gccccgaaca ctcgaaatcc cggatatttc ggatattccc ttttcctatg
     2221 ggaccatttt cttcaccctg gcggatttat tactagtttt ggtcgcctgg gggatcattt
     2281 ccatactcga actctacctg tccaggtcca aagtcggaag agagattcga gccgttagaa
     2341 gcaatccaga gctggccacg agcgtgggga tcagaaccga gaaagctttc ctgatcactt
     2401 ttgccatcgg ctccatgctg atgggactcg gatctttttt tgtcaccatc caatcagccg
     2461 ccacgcctca catgggcttg aattatgtcc tcatggcttt catcgccacc ttcctcgggg
     2521 gcgttggaaa agactgggga attgctcttg caggtttggc catggggtta gtcgaaaacc
     2581 tccctttggt ggtgctgccg gcccattata aatatatcgt ggtcttcgca atgatgttcg
     2641 gctttctgct gatgaagccg gaagggctcc tgggggcgag aaatcgcgtg aggagatgaa
     2701 caagaaagga gtttgacggt tggattattt ggcccacatt attaatatgg tccttatttt
     2761 tacgatgctg gcctcctctc tcaatctaat tctcggctac gggggaatgg caaccatgtg
     2821 ccatgccgcc ctttttggga tcggggggta tgcctccgcc atcctcacca tgaacttggg
     2881 gtgcaatttc ctggtaggaa cgattctcgc ggccctgctg tccgggttca tcggtgtcct
     2941 cttggcatcc ccctcgctgc gcatacagga cgagtacctg atcctgttta ccactgcttt
     3001 ccaaatggtg atttggggac tcatggtgag tgaggtttgg attaccaacg gcgagacggg
     3061 attgtccgcc atccctcggg ccagcgtctt cgggattcaa tttctaaagc cgatttccta
     3121 cattccgtta attgccgctt tggcagcgct tttcttcctg atctgctgga aggtaagcca
     3181 ttccccgttt ggacgcgtgt tgcgctgcat tcgggaagat gaactttcca ctcgctccat
     3241 cggaaagaat gtcctccgtt tcaaagtgct gactttcatg gtcggcgggg cgatcgccgg
     3301 gggagccgga agtatcctgg cccattataa tgcttatatc aatcctgtct ccttcaattt
     3361 ggatgcttcc attctgatga tcgccatggt cgttttggga ggttccacca atatgttcgg
     3421 ttctgtggtc ggaagcctgc tcctggtggg aatcccggaa atgctccgct tcgtcccggg
     3481 cacggccacg ctcattgcgc cgctacgcaa tgccatcttt ggcagcctgc tgattttttt
     3541 tatattattc cgtccccaag ggttaatccc tgaacacgtc ggccgcctgc gtgtccgccg
     3601 tacccccctg ccgaaattac ccgaggaaga gatccttacc ctttcggagc aagaaggtcc
     3661 ggcgaaatcc cagggggtga gcgaaccggt tatcgaggtc aaagggatcc ggaagagctt
     3721 cgggggcatt caggcggtga attcgttttc catgtccttg agcccgggca agatcaccac
     3781 gctcatcggc cccaatggtt gtgggaaaac cacggttttc aatttgatca gcggattcat
     3841 ttcccccgat gaggggtcca tatacttgcg cggaaaggac gtgaccaacc gccaacccca
     3901 tgaactggtc caattggggc tagtccgatc gtggcaggat gtgcggattt tccggaatat
     3961 gtccgtcctt gacaacgtca tggccgcgat tcccgatcaa atcggcgaac gtctatttgc
     4021 cattttcttt cggccccggc gggtaaggca agaagaatgg aaaaatacgc gtatggccct
     4081 agcctatctc gaatttgtcg gtttagcgga aaaggcctac gaactggcag gggagctttc
     4141 ctttgcggaa cagaagcagc tttcgctcgc tcgcctcatc gccacccggg gatccatctt
     4201 gctttttgat gaaccggctt cgggaatgga tgtggtgtct acgaaaaaca tacagaaaat
     4261 gatcctccgt ctggcgggat tcggtaagac catttgcgtc gttgaacata acctcgacct
     4321 agtcaaagaa ctgtcggacg aatctctctt catgaaccag ggagacgtaa tacggaaggc
     4381 taaaccggag gaactcatga ccgacccgca actggcgctc gtttattttg gaggttgacc
     4441 ggaaatatgc taatggaaat gaaggctgtt tccgcaggat attaccggaa acgagtcatc
     4501 catgacatct ccctgtctgt cgaccgatct gaggtcgtca gcctaatcgg acacaatggc
     4561 gccggaaaga ccacgactct gaaaaccatc ctgggggtca tcaagtcgga gcagggagaa
     4621 attcgtttcc gggacaggcg catcactaac caggatcccg tcgaaaatgt gaccgcggga
     4681 atacgtttta ttccccagga acggtttacg ttcccggacc tcacggtctg ggaaaatatt
     4741 atgttgggcg ctcacctcgt gcagcagaaa gagaagattg ggaaaagcat tgatgaggtc
     4801 tataaccagt tccccatcct gaaaaagagg ctgtcccaga aagcaggaac catgagcgga
     4861 ggggagcaga gaatgttgtc catggccatg gccatgatgg cacagcctca atttgtcatg
     4921 gtggatgaac cttccctggg tttagccccg ctaatcattc aggaaattgg aagaatcata
     4981 gggcgaatgg gggatcaggg catggcagtc ctcctggtgg accagaacat caagcagacc
     5041 ctgaaaatca gcaacagggt gtatgtcatg aagaacggtc aaatcgtact tgaagagacg
     5101 agccaaaaac tcctggatcg cggcgagtgg tgggaccttt attgaacgga aaggaagatg
     5161 tgggacattt gaattggatt gcttgggccg ggagtacctg gcccaagtgc ggtaggtagg
     5221 cgggttttaa taaaaacgga ggaggcaggt atgagaaaat cgattggtgt aggagctgga
     5281 acggttctat tgggggcagc cctattgatg atcgttccaa cggcggttct tgggcagacc
     5341 aaggccccgg taaagatcgg cgtgattctg cccatgaccg gtccggcggc gctttacggg
     5401 gaacaggcca accggggggt ccagcttgcg gccaagatgg tcaatggggc aggcggcatc
     5461 cttgggggga ggcaggtcag tattatcctg gaagacgacc aggccaatcc agtagagggc
     5521 gtcaacgcgg tcaagaaact catgaaccgg gatggaacga agctgatcac tggaggagtg
     5581 aacagttccg tatcgttggc ggagctccag gtgacgaagg aagccggctt gctccacatc
     5641 gtgacggttt ctacagcccc cgccatccgg gaacaggggc atccctccct ttttgccctg
     5701 cagccgacaa acagcatgaa agacgatttt gcgctcccgt ggatcgccca ggtattgaag
     5761 cccaagaccg tcgtccatct cctggagaat acggattacg ggagggttga agtggaggag
     5821 ttgaaaaagc actggggagc ttcggggccg aaagtcgtcg ccacggagac tttccagctg
     5881 ggagaaaccg atttcaccat ccacctgaac aaattcaaag ccatgaagcc ggaccttttc
     5941 tacctctcca ctgcggcccc gagcacgatc gggacgatct tgaagcaggc ctacgaaatc
     6001 gggttcaaag cccccatctt tctggctccg ggaaatttga acaatgatgt cgttcgcctt
     6061 gccgggccgg ccgcggaggg agtgttgtgc gccgactatt acgtcaacat cctggatacg
     6121 ccggcgaaca agaagtttgt gaaggaccac gaggagatgt tcaagaggcc ccccgaaaag
     6181 atggagatgc tgggcttcga atccattacc attctatcca aagctttgga catggccgga
     6241 acggaaaccg acgtcaagaa aatctccgat acgctgagac aaaacaagtg gcagactccg
     6301 cgtggcgaaa ttgtcttcga tgcgaaagga caggccaaag gggttggcaa ggaccaggct
     6361 ctggtggtga aggatgggaa aattacgata aggaaatgaa cctcaggtcc tgtaaaaacc
     6421 cttgagcctt cttggcttcc ggggaggcat ggacccgcgt cagaacggcg tccggtccat
     6481 gtccctcccc accatcgggt ctacctccat gatcgatctc gatctcctcg ttatccagct
     6541 ggccaatggc ctggtcatcg gaatggggta taccctcgtg gcgatcggcc tgacaatggt
     6601 cttcggcatc ctgggggtcc tgaatttcgc ccacggcgag tttttcatgc ttggggcttt
     6661 tgtcgcttac gttttcggcg agctcatggg ctggccttac gtcgtgtcgt cggtcgtcgc
     6721 catcgttctg gtcatggcga tttcgtttct ttttgagctt atcagcgtcc gtccctttat
     6781 ggaacgggac ccggaaatga ccctcctctc caccttcgcc gcgagcctca ttctgatgaa
     6841 cctggtggag gtcatctggg ggaatacacc gcttccgctc aacaccccgt tttcaaaacc
     6901 gttggatctc ctgttcctgg tcttgaccgc gcagcggatt ctcattctgc tggtcggggc
     6961 agtgggggtg atcctgctcg ggctgttttt gaaattcacc aaaatgggca aggtgattcg
     7021 agcgacggcc cagaataaga aggcctccgc cttagtgggc atcaacatca aaaaggtcta
     7081 tcggctcact ttcgccgccg gcacgggtct agccgcaatt gcaggggtac tgattgcgcc
     7141 cctgtccaac ctgtatgcat ccatgggcca ggggatggtg ctgaaagggt ttgtggtcgt
     7201 gattctggga ggacttgcca gcgtcccggg ggctattctt ggaggattga ctctgggaat
     7261 aacggaagca atagctgccg ggtacgtttc cagcgcgtgg aaggatgtga tcggattcag
     7321 catcctcatc atcatcctgc ttcttcgtcc gcaaggcctg atgggaagga gagaggcatg
     7381 aaccgggccg caaggaaccg gagaaactgg acgcgattcc tgggtttggt ggtggccttg
     7441 ctgctgccca tcgtctttca ggatgcctat ttgcggaata tcctggtcat attcgggatt
     7501 tactgcctat tggctctgag cctgaacatc atcgtcgggt acatcggcga agtaagcctc
     7561 ggtcatgccg ctttcttcgg actgggaggt tacgcttcgg caatcttatc aaagactgtg
     7621 gggttccccg ttctcctttc catacctagc gcggtcgtca tctccgggat tctgggcctg
     7681 ttcatcgggt atctcgcctt gcgcctgaga gggccatatt tcgccatcac cacgctggct
     7741 ttcgctgaga ttataaggct catcgtcacc aactgggtcg acctcacgcg ggggcccctc
     7801 ggtttcactc aaatccctcc cctggtcctc ggaattccag gctggttcga attcaaggtc
     7861 atgagcgaac tgcgctacta ctatttcgtc ctggttctgg tcggcctggt atttcttctg
     7921 atttacaacg tagttcactc tccgacgggc cggacgtttt tggccatccg cgagcaggaa
     7981 cccctggctc gttctgtggg gatcagccct tttcgctacc gggttctggc ttttgtcatc
     8041 tctaccatga ttgccggctt ggccggagcc gcttatgctc attttttcag gatcatcagt
     8101 ccggacatta atatggtcta ttattctgcg atcccgctga tgatggttat ggtcggcggc
     8161 cggggaaccc tgctggggcc tttcctgggg gctcttcttt ttgctgttct gcccgagctt
     8221 ttccggaccg ccggaactat ccggatggta ttcttcgcct cttttctgtt ggccaccatt
     8281 gtgtatatgc ccgagggcat tgcggtgaaa attcagacct ttattcggga ttgggagacg
     8341 aagaaatcgc cccaggaact cggggatgtt tagtagagaa gctactcatg cctattttgg
     8401 aaggtaagaa tctcaccaga gcgtttggcg ggttgatggc cctgagagag gtgagcctgg
     8461 aggttcagcc tcacgaaatc ctgggcatca tcggtcccaa cggggcgggg aaaacgacct
     8521 gtttcaacat ccttaccggc ttttattcag ccacgagcgg aaccgtcttt tttgaaggcc
     8581 aggaggtctc ctccatgccc atggaaaaga gggccctgct ggggatggtc cggacttttc
     8641 agcatacaag ccttttccca aaattgacgg tttatgagaa tgtactgacg gccaattata
     8701 aaatgaatgc gacgaatctc ttgcatcata tttttcggag cggggccttc aaaaaaacgg
     8761 aaagggaatt agacaagaag gccatggaag ttttggaatt cctgggcctg cgggaacaac
     8821 gggacatgat ggcccgcaac cttccctatg gatcgcagcg caagctggag atcgccaatg
     8881 ccctcgccac ggatcccaaa ctgttgctgc tcgacgaacc ggctgccggg atggatccgg
     8941 ttgaatccca ggacctcatg cggctgatta aggagatcca gaagcgagga atcacgatta
     9001 tgctggtaga gcacaatatg aaactggtca tgggaatctg cgaccgcctg gtggttttga
     9061 attacggctc aaagattgcc gagggaaagc ccaaggatat tgcccagaac ccggacgtga
     9121 ttaacgtata tttgggtaag aagcaggact cctttcgcct gaaaaataga ggttagctaa
     9181 tctttcatgt taaaacttga atctgtgcgg gtcttctatg ggaatgttca ggccctgaag
     9241 ggaatatcca ttgcggtgga agatcacgaa ctggtcgccc tcatcggcgg gaatggggcc
     9301 gggaagagta cgacgctgct gacggtctcc gggttaaccc gccccaggag tggcgaaatt
     9361 tcctaccgga gacaaaggat cgaccacctg ccccccttca agattctgga gatgggcatt
     9421 gctcatgtgc ccgagggccg ggagatcttc ccggaactga ccgtgctgga gaatctggaa
     9481 atgggggctt atcggtctcc cgacgaggaa tcctcccaag ggcagaagtt aaaggagatg
     9541 tacggctact tccctgtcct ggaggaaagg aaaaagcagt tgggtagaac cttgagcggc
     9601 ggggaacagc agatgctagc cattgcccga ggcctgatgt cctcccccaa gctcctcttg
     9661 cttgacgagc cttctttagg tttagccccg ctggttgtcg atcggttggc cgagattatt
     9721 ttgcagttgc acgaaaaagg gttgggaatc cttctggtcg aacaaaatgc gcagatggcc
     9781 ctcggtatgg ctgaccgggg atatgtactc gaaacagggc agatcgttct acaaggcccg
     9841 ggcagggatc tattgaacga cgaaaacgtt caaaagtcct acttggggct ttaggaggaa
     9901 ttgacatttt ctccaactcc gtgttaagtt ggggcggttt tttttggtca ttggccaaga
     9961 acttttgaag attccacggt aaaaggaggg aaagatgaaa aaggccggat ttttgtttgt
    10021 ctgtatgttg gttcttattg gctgggcttt accgggcaac gcccaaaaac ctgcggggaa
    10081 acccatactt attggataca tcagccatct tacgggcccg gcggcgaagg tctctcagca
    10141 ggaaattgca gcggctcaag ctacggctga catgatcaat gagaaagggg gcgttctggg
    10201 caggcccctc gaactgatca ctcgggatga ccataccgat gccggcgtcg ccacccgggt
    10261 ggcgcgcgaa ctcatcctgg acaaacgggt taatttcctt gccggatgca gcaataccaa
    10321 atcctcccta gcgatttccg aggtggcgaa acagcaaaaa gtgatctaca tcattgctac
    10381 ctctcaaacc gcagccatta ccgaggaact cgggcatcgc tacgtattcc gctcctggag
    10441 caactcgtat agtcagatcg ggccggcggc cctctggatg gccaagggaa aattcaagag
    10501 attcgtgacc agtgccgggg attatgagtg ggggcataac agtgtagaca cgattctatc
    10561 cctggtaaag aagcatcgaa cggattttga ggtcctgaaa caggtgtggc cgccctatgg
    10621 cacaggggac tacagccctt atctttctca actcatggcg gtcaaacccg acctgattat
    10681 tgccgctcac tatggatccg aaacgaccaa ctttatccgg caggcaaagg ccttggggct
    10741 ggtggggaaa ccggattcac cgcagctcat catttatatg gaccaggaca acaaggaagc
    10801 cctgggaaaa gaaatgcccg aggggatcgg cggttattgc gccttccata ctcgaagtat
    10861 aaataatccc tgggcggatt ggtttgacaa tgtcgtccat aagagaacgg gaagctggcc
    10921 cgggggaatt gccctgggag gatatatgtg tgtttcctgg ctggccaagg gtgttgaaaa
    10981 ggcaggaacc accgatacgg aagcagtcgt aaaggccttg gaagggctgg agatggaaac
    11041 ctacgtgggc aaaatcaaga tccgggaatg cgaccatcaa gccacgggcg tacagcctct
    11101 cggtttcacc aaagcgagct cggattaccc gttttgcgta atgaccgacc tatattaccc
    11161 tccgggagag cagtttttgc atacctgtaa ggagatcgaa gctctccgag ctcccaagaa
    11221 ataggacatc gaaagaagcg gcggattccg ctgggccgtt agggcttatg ctgcccgggt
    11281 cgtctgatca tggagagcgg acgacccgga tcgcctccgt aaatctcccg gtggcaagtg
    11341 gagttcaggg gaaaaagaac gcaggcaaat tttcggatcg aggtcgtggg aactatggta
    11401 gacctgatta taacccaaat ccttacaggc ataggggccg ccggcatatt gctgttgatg
    11461 gcggtgggat tgagcctggt ctttggggtg ctgcacattt tggattttgt ccatggaagc
    11521 atcttcctct tcggcggata catcggatac actttttcca tcctcctggc gagggtggag
    11581 gggggattct ggttgagcct tgtggccggg ggcctgatgg ccgccgccct ggggaccctt
    11641 atagaaattt tgtttctccg gcgggtctat gaccggcagc tcctgctcca gttcctgatt
    11701 ttattcggcg tggtttatat cgtctcagat gtcatgaaga tcttctgggg aacgggctac
    11761 ctatccattt cccggcccga cttcctctcc gattcgatga gctatgccgg agggagagtt
    11821 gtggtagcca ggtataatat ttttgtaatt gttttggggg ccgcgaccat cgcgctgctc
    11881 tggcttctta tgaaccgcac ccggtttggc cttcaggtga gggccctcca tcagcaaaag
    11941 gagatggccg cactcctggg gatgaatacc cccatgctgc gtacttccgt ctttgccatc
    12001 gggagcggca tggctggcct ggctggggtt gcgatcggag ggcttggcag cttgagccca
    12061 ggtatggatg tggaagcggt gatcctggcc ttttccatcg tcaccatcgg aggactgggg
    12121 agcgtaaaag gggccattgt gggggccctg attgtgggcc tggttcagtg cctggggctt
    12181 ctgatcctgc cccagctgaa catcactttc atctacctgg tcatggccgt ggttttggtg
    12241 gtccgtccct ggggtctttt cgggagggcg acggcttagc ttagaaaggc cgctttttgt
    12301 tttgttgccg gggcgctgga agggaaattt catgggcggg atgagtagca aaaacgggtt
    12361 caccttagtt gtgattctgg ctgcgctctg cctgttgccc tgggtggtct cccccatgat
    12421 cattctcgtt cttagcgaag ctttgatctg ggttctattt gccattgcca caaatatcgt
    12481 cttgggctat gggggtcgga ttaccttcgg tcatgccgct ttctttggca cgggcggata
    12541 cgtgtgtgcc ctcctgctca agaaattgga ggcgggactt cccgcgggaa tcttttgcag
    12601 cgccgcagtg tccacgggta tggggctgat catcggttgg ttctgcgtgc ggctgaccca
    12661 tgtttacttt gccatgctga ccatggcctt cggccaactg gtatgggcgg tggtctaccg
    12721 gtggcgttac gcgggagcgg atacgggcat catcggggtg ccgggcctcg gctcccccgt
    12781 ggaattttac tttctggttc tcgtcctcgt ttccctttcc gtcctggtca tctacttcat
    12841 cgtccattct cccttcggaa aggttttggt ggggacgcgg gaaagcgagg ttcggccccc
    12901 tttcatcggg atcaacgttc gacggcagca gctggtcgct tttgtgatct cggctttttt
    12961 ttcgggaatt tcgggcggcc tgttcgtagt cctgaacggc atcgcctatc cggatatttt
    13021 aacctgggtt tattccggcc aggtgctggt catgtgcctg ctggggggga tgaccgtgtt
    13081 gctgggcccg gcggtagggg cggttatttt gatctttctg cagcattgga ttactggcta
    13141 tacgcaatat tggccgctgg tgctgggttt gattatcgtc cttttggttt tgttcctgcc
    13201 taatggaatt ttgggtagcc tcctggaccg agggcggaga ggacttgaat tacccgtctg
    13261 gctccgtccg caaaaatgac gcccggccct gcggaaggtt cctcactttg ctccacccgt
    13321 ggtcgggtgt cggtacgaca gcgtaggtac gataatcgtt ctgatccgtt ccgcctctca
    13381 tcgggcctcg ctgtttcggg aagagcggtt caacgtactt tttcaccgag ctgctaagga
    13441 atggaaggca atccgtggct ctcctggaga ttcaagacgt aaccaaggca ttcggcgggg
    13501 tccaggccgt agaccatgta accctcagtt ttgaagagag cggccttacc tcagtgattg
    13561 gccccaacgg cgccggaaaa accacgcttt tcaacctcat cacggggcac ctcactccca
    13621 gcgggggaag gatcgtgttt gcgggggagg atattacgcg gcttccctcc tttgaaattt
    13681 ggtataaagg gttatgccgt tcctttcaga tcaccagcct tttccccagt ttgaccgtgt
    13741 ttgaaaacat tcagctagtc cagctgaccc gtcatcggaa acaattcaac ctctttcaac
    13801 gggccgccca tctctaccag gaagagacct tcgagatcct caaagccgtt gagctggcgg
    13861 accaggcgaa tatgacggtt agttccatgc cttacggcga tcagcgcaag ctggagatcg
    13921 gaatcgtgct ggcatccaac gccaagttga tgctgttgga tgagccgacc gccggcatgg
    13981 ctcgggtaga aaggctggcc atggcgggtt ttatcgagcg gctcttccag gaacggggcc
    14041 tgtccatcgt atttatcgaa catgatatgg atgtggtcat gtccatctcc caaaagattt
    14101 gggtcatgca tcagggaaga attatcgtcg agggtacccc cgatgagatt cggagaaacg
    14161 acgaggtcag gagaatctat ctgggcgagc aaaacctatg gtcctcttag aaatcgaaaa
    14221 aatcaatacc ttttatggcc tgagccatat tctctttgat gtctcattga agattgagaa
    14281 gggggaagtc gtctgccttc tcggacgcaa cggagcggga aaaacgaccc tgatgcggag
    14341 catcatgggt ttgaatcccc ccagatcggg aagcattcta tttcagggtg agcccatcat
    14401 aggaaagccg acctttcgca tcagccgggc ggggatcggg atggttcctg aaaacaggct
    14461 catttttgcc gacctcaccg tgcgggaaaa tctcgaggcc ggctggagag ataggggaga
    14521 aaacggatgg acggtggaga aggtctggaa tctattcccg gagttgaaaa gattatcctc
    14581 gaaccgcgga gggcagctga gcggcgggga gcagcagatg ctaagtattg cccgaaccct
    14641 gctgcagaat cccgctctcc tgctgctaga tgagccgaca gaggggctgt cgcctcttct
    14701 ggtccatcgg atcgcggatc gggtgttgga gattaaaagc ggtaaagtcc tgaccatgtt
    14761 aatcgccgag caaaattatc ccatcgccag caaaatgggg gatcgctcct atattttgga
    14821 gaagggcctg atccaattcg aaggcactat gaccgaacta aggcggaatc ccgaggtgac
    14881 gaaaaaatat ctggcgattt aggaattggc caggggatac aatctggccg gcgccaccat
    14941 cgcgacccag gggttggcca gccagccaat cgtgctggcc ggttcagcca agatgcacaa
    15001 gaagtttttg cccgggatcg cttcagggga aataattccg gcgttcgcct tgacggagcc
    15061 ggaagccggc tccgatgccg gcaggctaca gaccaaagcc atgcgggaag gtgagcattg
    15121 tcggatcagc ttgaagtccc cctttcgatc taatacaatc ctaataaagg cacggtttcc
    15181 ccatcaaaat ccttatccat caaccctagc ttttaccctt cccgggtagc caggaggtgg
    15241 catggtgtga aaggttg
//
LOCUS       DASPRR010000481         5354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2053538, whole genome shotgun sequence.
ACCESSION   DASPRR010000481 DASPRR010000000
VERSION     DASPRR010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053538"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VEL68_17710"
     CDS             complement(<1..2079)
                     /locus_tag="VEL68_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HXZ37864.1"
                     /translation="MKKRGGFPLYLLLSLPILLLPGLASAIPQTIQTERLEEICPIVE
                     EAIRGGQTPGAVIMVGHQGKTVFRQAFGCRAYEPRNEPTTPDTLFDIASLTKVVATTP
                     ALLQLVEKGTLSLDDPVVKHWPKFRGQRKEQITIRQLMTHYSGFRASLPLKPDWSGYD
                     AALKKITAEKVIHPPDSLFLYSDINFIILGELVRRISGQSLDAYCRRNVFEPLGMKDT
                     LFNPPPSLFGRLAPTKENQPGEVHDPTAHRMGGVAGHAGVFSTADDLAILAQALLDGG
                     RFPGGQILSPRTVEDMILPQSSPEKIPWRGLGWVIHSPSPFPQGSNWSEWLPPGSFGH
                     KGYTGTLIWIDPVSRTYLIVLTNRVYPGGKGDQENLRDQVFSIVTQALGRATPASLAG
                     QPLTPQSGGPSPEAPSRVRTGLEFLAAQNFAPLAGKRVGLITNQTGRDSSGRRTIDLL
                     YAAPKVKLKAIFTPEHGLSGQAEGKVPSGRDEVTKVRVYSLYGDDLRPTAKMLKGIDV
                     LIFDVQDAGARFYTYLSTLGYAMEAAAQNRIPFYVLDRPNPINASLVQGPVMDGDLKS
                     FTGYFPMPWRHGMTLGELARMFNEENKIGAQLKVVPMIGYQRMAWFDETGLPWVNPSP
                     NLRSLTQMALYPGVALVEGANVSVGRGTDKPFELLGAPWIKAQELADDLNSRNIPGVE
                     FRPATFTPDGN"
     gene            complement(2115..3428)
                     /locus_tag="VEL68_17715"
     CDS             complement(2115..3428)
                     /locus_tag="VEL68_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HXZ37865.1"
                     /translation="MGKRGRNIALFVFVWLFFPASGPAADYPGLKVVDHQTGQPIVNA
                     KVTLNHEVLLADSQNLFPLTKAGGMVRVRAHGYQRLEQVLPASFPASPYTLRLVPKNP
                     KALYLSFYGIGEKALRDPALKLIEETELNALVIDVKGDRGWIPYKSSVPLAAEVGATK
                     ITILKDMPGLIKSLKERGIYTIARIVVFKDHPLATTRPEWAVKKEGGEIWKDRENLAW
                     VDPFRKEVWEYNIQIAVEAAQQGFDEIQFDYVRFPDSSSPRFSQPSTEESRVKTVSGF
                     LQEASNRLLPYNVFVAADIFGYVCWNYNDTKIGQKLEAMVTPVDYLSPMLYPSGYQFG
                     IPGYRNPVANPYEIVYLSLKKAQERTNLPSIRFRPWLQSFRDYAFDRRVFNGKEIREQ
                     ISAADKFGSQGWMLWNPGNVYSSEALKVEKSPTWITSLASVPERL"
     gene            complement(3412..4236)
                     /locus_tag="VEL68_17720"
     CDS             complement(3412..4236)
                     /locus_tag="VEL68_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXZ37866.1"
                     /translation="MTKKTIFLMLFLAVLDFVPVLRLVNPSWAGEQGVPILAYHRFGP
                     VLADSMTVTTQVFESHLKHLQENGYRAILLRDLLEEYLKGGTPPSSRLVGLTADDAHI
                     SVYATALPLIKKYRIPMTLFVYPSAVSNASYAMTWDQLRELKATGLFDFQSHTYWHPN
                     FNKEKKRLAPAEFEKLVEMQLRKSKEKLEKELNVRVNMLAWPFGIHDPWLMDKAAEAG
                     YAAAFTIEGYASCKEDHPMAIPRYLMVDSDRGKAFERIIHGSILTSKKGNGNGKKG"
     gene            complement(4625..4903)
                     /locus_tag="VEL68_17725"
     CDS             complement(4625..4903)
                     /locus_tag="VEL68_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37867.1"
                     /translation="MKSRTVEELLLELDLTEEEKGKHAKLIQESLERQKRLIEYRKEG
                     EEGVRALEIYLRTLSRALLVIHQSVQQVNDRLAGVMLQNLPESKMPRA"
BASE COUNT         1255 a         1391 c         1433 g         1275 t
ORIGIN      
        1 gttaccatcg ggtgtaaagg tagcaggccg gaattccact cccgggatat tgcggctatt
       61 aagatcatcc gccaattcct gggctttgat ccatggagcc ccgagaagct caaagggctt
      121 atccgttcct cttcccacgc tcacgttggc cccttccacc aatgccacgc cgggatacag
      181 agccatttgg gttagggaac gcaaattggg cgatggattc acccagggaa ggccggtctc
      241 atcaaaccaa gccattcgct ggtacccaat catcggaacc accttcagct gcgccccaat
      301 tttattttcc tcgttgaaca ttcgcgccag ctcgcccaag gtcatgccgt gacgccaggg
      361 catagggaaa tatccggtga aagattttaa gtctccatcc atcaccggtc cctgcaccaa
      421 gctggcattg atcgggttcg gccgatctaa aacatagaag gggattctgt tctgggccgc
      481 agcctccatg gcgtatccca gggtggagag gtaagtataa aaacgggccc ctgcatcctg
      541 gacatcgaaa atcaggacat cgattccttt gagcattttg gccgtggggc gcaggtcatc
      601 tccatagagg ctgtacacgc gcaccttggt gacttcatcc cgcccggaag gaaccttgcc
      661 ttcggcttgg cccgaaagtc catgctcagg ggtgaagatg gctttcagct tgaccttggg
      721 agcggcatag agaaggtcta tggttcgtcg tccggaagaa tcccttcccg tctgattggt
      781 aatcaaccca acccgctttc cggccagagg ggcgaagttc tgggccgcca ggaactcgag
      841 cccggtcctg actctgcttg gggcttccgg actcggcccg cctgattgcg gggtcaaagg
      901 ctgtccggcc aggcttgccg gcgtggcccg gccgagtgcc tgcgtcacga tggaaaagac
      961 ctggtctcgc agattttcct gatccccttt tccgccgggg taaacacggt tggtcaggac
     1021 aatgagatag gttcgggaaa cagggtcaat ccagatcaaa gtcccggtat accctttgtg
     1081 gccgaatgaa cccgggggca gccattcact ccaattggac ccttgcggga aaggagaggg
     1141 agaatgaatg acccatccaa gacctctcca ggggattttt tcgggcgaag attgaggcag
     1201 gatcatgtcc tcaacggtcc ggggactcag aatctgacct ccgggaaaac gacctccgtc
     1261 gaggagggcc tgggccaaga tggccaggtc gtcggccgtg gaaaagaccc cggcatggcc
     1321 tgccacgccg cccattcgat gggccgtggg gtcatggacc tccccgggtt ggttttcctt
     1381 ggtcggagcc agccgtccaa agagagacgg aggcggattg aagagggtgt ccttcatccc
     1441 caggggctca aaaacattcc ggcgacaata ggcatccaac gattgtccag atattcggcg
     1501 gacgagttct cccagaatga tgaaattgat gtcgctatag agaaaaaggg agtccggggg
     1561 gtggatcacc ttctccgcgg tgattttttt cagggctgca tcgtaccccg accaatccgg
     1621 cttcagcggg agacttgccc ggaatcccga ataatgggtc atgagctggc gtatggtgat
     1681 ttgttccttt ctttggcccc taaacttggg ccagtgtttg accaccgggt catccaggct
     1741 caaggtcccc ttttccacaa gctgcagaag ggcaggggtc gtagctacta ccttggtgag
     1801 ggaagcaatg tcgaacaagg tatccggagt ggtgggttca ttccgcggtt catacgcccg
     1861 gcaaccgaag gcctgccgaa agaccgtttt cccctggtgg ccaaccatga tgaccgcacc
     1921 cggagtctgg cctccccgaa tcgcttcctc gacgattgga cagatctctt caagcctttc
     1981 ggtctggatg gtttggggaa tagctgaagc gagcccaggc agaaggagaa tcggcaggga
     2041 caataataga tacaggggga agccgcccct tttcttcacc ttcagtgatc cttctatctt
     2101 ctattggaat tattttataa tctttcggga acggaggcaa gactggtaat ccaggtggga
     2161 gatttttcca ccttcagagc ttccgaagaa tagacgttcc ccgggttcca gagcatccat
     2221 ccctgggacc cgaatttatc ggcggcgctg atttgctccc tgatttcttt tccgttgaag
     2281 accctgcggt cgaaggcata atctcggaaa gactgcagcc agggacgaaa gcggatcgat
     2341 ggaaggttag tcctctcctg ggctttcttc aaggataggt agacgatctc ataaggattg
     2401 gccacgggat tccgatatcc cggaatcccg aactgatacc ccgagggata aagcatggga
     2461 gaaagatagt ctacgggagt caccatggct tccagcttct gcccgatttt ggtgtcgtta
     2521 tagttccaac agacgtatcc aaaaatatcg gcagcgacaa aaacgttgta cggcaggagg
     2581 cggttggagg cttcctggag aaagccggag acggttttca cgcggctctc ttcggtactg
     2641 ggttgcgaaa aacgaggaga cgaggaatcc ggaaaccgta cgtaatcgaa ctgaatttcg
     2701 tcgaaaccct gctgagccgc ctcgacagca atttggatgt tgtactccca tacttccttc
     2761 ctgaaagggt ccacccaggc caagttttcc cggtctttcc aaatctctcc tccctccttc
     2821 ttcaccgccc attcggggcg ggtggtggcc agagggtggt ctttgaacac cacgattcgg
     2881 gcgatggtat agatcccccg ttccttcagc gatttgatca acccaggcat atcctttagg
     2941 atcgtgattt tggtagcgcc gacttcagcc gctaggggga cggaactctt gtaaggaatc
     3001 caaccccggt ctcctttcac gtcaatgacc agggcgttga gctccgtttc ttcgatcagc
     3061 ttcagagccg ggtcgcgcag agccttttcc ccaatcccgt aaaaagagag gtaaagcgcc
     3121 ttgggatttt tgggtaccag ccgaagcgtg taggggctgg caggaaagga ggcaggaagg
     3181 acttgctcga gtcgctgata tccgtgggcc cgcactctca ccattcctcc tgctttggtg
     3241 aggggaaaaa gattttgcga atcggccagc aaaacttcat ggttgagggt aactttggca
     3301 ttcacaatcg gctgaccggt ctgatggtca accaccttca gacctgggta atcggccgca
     3361 ggccctgacg cggggaaaaa taaccaaacg aatacaaaga gcgcaatgtt cctacccctt
     3421 tttcccattg ccgtttcctt tcttgctggt gagaatcgat ccatggatga ttctctcgaa
     3481 tgcctttccc cggtcggaat caaccatgag gtaccggggg atggccatcg gatggtcttc
     3541 tttgcaggag gcatacccct cgatggtgaa ggccgctgca tatcctgctt cagcggcttt
     3601 atccatcaac cagggatcat gtatgccaaa gggccaagcc agcatgttga ccctgacgtt
     3661 caattctttt tccagctttt ccttggattt cctgagctgc atttccacca gcttctcgaa
     3721 ttctgccggg gccaaccttt tcttctcttt attgaagttg ggatgccagt acgtatggga
     3781 ctggaaatcg aagaggccgg tggccttcag ttcgcgaagc tgatcccagg tcatggcgta
     3841 ggaagcattg gagacggccg aaggatagac aaaaagggtc atcgggatcc ggtacttctt
     3901 gatgagggga agagcggtgg cataaaccga aatgtgggca tcgtccgcag tcaatcctac
     3961 gaggcgggaa ctcggaggag ttccaccctt gaggtattcc tccaagaggt ctctaaggag
     4021 aatggcccgg tacccattct cctgcagatg tttcagatgg gattcaaaaa cttgggtggt
     4081 gacagtcatg ctgtcggcca gcacgggacc gaagcggtga taggcaagga tggggacccc
     4141 ctgctctccc gcccaggaag gatttaccaa tcgcagaacc gggacaaaat ccagcactgc
     4201 gaggaacagc ataaggaata ttgttttctt ggtcatgact attattcata tcgtcaagaa
     4261 tattttccta attcacccat ttgttaggat atggaagaat tattgggaat ttttttatta
     4321 attaaataaa atcaaactgt tagttctcat tcactggcag gaaatttagc tttatacccc
     4381 tatttcattg aagattttaa aaataacggc cggaattgag ggatcacccc ccactttgac
     4441 agatgacgtg cgaatgaaat aactcccggc tcaacccttt gcaggcctgc agaatgactt
     4501 gggggaacca caatatgaag atttttaagg tcaacaggat agagaggtgg gttgaagcgc
     4561 cacagataaa aagattgaag ggcaggaaat ctctgaaatt aggaaccgtt tgaggccgga
     4621 gcctctatgc tcggggcatc tttgattcgg gcaagttctg aagcatgacc cctgccaaac
     4681 ggtcattcac ctgttgaacg gattgatgga ttaccagaag ggcccgagaa agggtgcgca
     4741 gataaatttc caaggccctt accccttctt cgccttcttt gcgatattcg atgagccttt
     4801 tctgccgttc cagggactcc tgaatcagct tggcgtgttt ccccttttct tcttcagtca
     4861 ggtcaagttc aagcaacagc tcttcaacgg ttctgctttt cattggaggc tcctttcgcc
     4921 tcatagaaat tattctttcg gttctgctca ttcaaaaaaa agagcgccag tgaggccagc
     4981 ccgggatatt cgccccgggg tcaccttaaa ccctccttct cccctccata tgcccttaat
     5041 gggtgaaacg agactttaaa cctgtccacc cgctggctaa tatttaagga ggattccctt
     5101 gaagattcta ccggggaaga aagcgagggc cttcggagaa ccttttcaag ggatgaccgg
     5161 gcactccagg aaaaatggtt gatcaacaag atggacatca gccatagaaa tagaaacatg
     5221 atgatccaaa actttctcat ctgagtccct ctccatcccc ttcaatagtg ggaagtaata
     5281 ctccttcatt tagcaagact cattccaaag agggaagacc attcaatgat caatggtttc
     5341 catcggttga ggtt
//
LOCUS       DASPRR010000482        10103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1082880, whole genome shotgun sequence.
ACCESSION   DASPRR010000482 DASPRR010000000
VERSION     DASPRR010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082880"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VEL68_17730"
     CDS             <1..201
                     /locus_tag="VEL68_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37868.1"
                     /translation="VASFGGLPEDVPVVGDWTGDGIAKLGIYRQGQWFKDLNNNGTWD
                     GCGVDACIESFGGFALDIPFVK"
     gene            complement(269..919)
                     /locus_tag="VEL68_17735"
     CDS             complement(269..919)
                     /locus_tag="VEL68_17735"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ37869.1"
                     /translation="MHLHFHLPFFTMKLNWAERWAVNNPSRPIQQRLEIRWMKRRRPL
                     APGSVVLEVGCGRGVGAALIQKEFQPAVLQAMDLDPEMLRRGKTYLPEGERKEISFYV
                     ADVRRVPARVESLDAVFGFGVLHHVPDWESALMEIVRVLKPGGVYYLEELYPSLYQNF
                     ITKHILLHPADRRFRSEDLKTAIKGAGLRLDHLIEHPKVGILGIASKSSGQKGNGK"
     gene            complement(933..1667)
                     /gene="ubiE"
                     /locus_tag="VEL68_17740"
     CDS             complement(933..1667)
                     /gene="ubiE"
                     /locus_tag="VEL68_17740"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HXZ37870.1"
                     /translation="MSFFGFRRIPAREKGRQVLRHFNSIAGKYDFMNTVLSFGLHYFW
                     KRWAVEGLSLNPGDRVIDVCGGTADLSILAGRRVGERGRVFLYDINRAMMEGGKPKVS
                     RASLSERVCFIQGDAEGISCPQGVFNAALVGFGVRNLTHMDKGLAEMHRVLRPGGKFM
                     CLEFSLPTSPALRRLYDFYSFHLMPLAGKILAGTREAYLYLPESIRKFPPPEELSRVL
                     REIGFTRVMHRRLTNGIAVIYTGVKG"
     gene            complement(1928..3316)
                     /locus_tag="VEL68_17745"
     CDS             complement(1928..3316)
                     /locus_tag="VEL68_17745"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ37871.1"
                     /translation="MQGSILDSFFVPRSVALIGATNNPGFGHGIPLFWRKNGWLDRAF
                     LVNPKGGEMHGKRVIPSISDLPGGVDLAVVIVPSPAVREVLIELGKKGIRAVIIESAG
                     FAEIGPEGQKRQEEIAALARGLGIRLIGPNCVGVVNTENRFATIEIMDSSMEPGPVAI
                     MAQSGVFGNILLDHLPAAGLKISKVATLGNKIDLNEADFLEYFAADPNTRVILVYMEG
                     TRDGAGFLESLRRTSRKKPVVILKSGRTPFGREATLSHTASLSGEDRVFDAAFRQAGA
                     IRAENLQEMIEIARVFTTQPPMKGKQVGVLTTSGSMGAMVADAAFMEGMRLPSWELNT
                     AERIKAKAPAWMNIKNPLDIGPSGIFRPACRAIFSDPNPDGFILVPVIPYAAIEIWVR
                     MGVKLRDMLGDWPVYRETIPDKPVIAVLLGYKEYVEQIRDLCGTGVSTVSSPESAVRA
                     LSALHRITPFSQ"
     gene            4023..4922
                     /locus_tag="VEL68_17750"
     CDS             4023..4922
                     /locus_tag="VEL68_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37872.1"
                     /translation="MPAREDYLSVIGQQEVSNWEWKRKDWALAKRVGHALSANYALVI
                     ERDFNAGINFYQTLLMNLETGKQFKVAFRVPKGREHREEWQQIGRVAYTEIFRDAKND
                     MLATAMRKGRVSVSGIPAPIDRGTKDPVGSRPADPSKERTILPPAPPKVREVALEESM
                     KEIPASSSIRLAIYDIHANEPLKIAALILSEALREEFFRLGRFMLVNRENMAQILNEM
                     GVQQTGLVEEKQAVQAGKGLAARQIILGQFGSIGNTLLLQVKRIDVETQSTLSIGSAK
                     CTPGKEDEVLAGLPELARRLAGK"
     gene            complement(4926..5798)
                     /locus_tag="VEL68_17755"
     CDS             complement(4926..5798)
                     /locus_tag="VEL68_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ37873.1"
                     /translation="MQYEGIVIRPPSEAESLILQVTLGCSHNKCTFCPTYKGRRFRIK
                     DLDQIKEEIDEMAGYGPFRKVFLADGDALIIPQPKLIAIFDYLNQMFAGLRRVGIYGN
                     AKSILRKSVEDLKELRDRKLGIIYLGVETGDPALLEKVRKGATYSQMVEAGRRVKEAG
                     ISLSTTVLLGLAGAQGGRQHAEATAKVLSDIDPDFASALTVMVVPGTPLYEEMKAGRF
                     QLPSPFEMLEELGTIVAGSNFSRCYFTSNHASNYLPIKARMPKDKEAVVNLIRKVVSA
                     KDKALLRPEFLRGL"
     gene            6155..7426
                     /locus_tag="VEL68_17760"
     CDS             6155..7426
                     /locus_tag="VEL68_17760"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXZ37874.1"
                     /translation="MKKGPFHFGLVLGLMALGGCAGSSGKTVARQTQQGAEFRPKRIY
                     AVEMKQAWDLTLKALKREGIPLEIVNKEIGVIRTDYQNLSPWERSKCDIRFSQEPEQN
                     THIYVLCRYEGRKDISEPFRDFTYSLPRQAMKAEEEIYRKLEPHILSYERIPPRQEET
                     SVKAAIPSAPVPYSKNPGPSAKTAMPSNPPAAVISPAAAQAKIETVSPTTSPVAEAEK
                     PQPAVGAAPPPRSKGEAATLTAAVLPKKAEIKEESLSAPLMTVAPTNVRVDPSTQSKI
                     AAVLKKGEQVEKTGESGSWTRIRLSSGENAWVFTDFLQPATSEPSLTSPAPAVQPSLQ
                     TKPGQARQILLAKPVEPDKKGIEGGAKIIFMTRDITKMWAEPNSRSEVVLVLKKGRKV
                     ERLTESGEFTKIRLSWGSSGWVLTRFLQTVP"
     gene            complement(7567..8307)
                     /locus_tag="VEL68_17765"
     CDS             complement(7567..8307)
                     /locus_tag="VEL68_17765"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXZ37875.1"
                     /translation="MKRLRYLKDNQVHYGMLEGETVYRLGSSFEGPNVKGKAVGPLPA
                     VKVLPPCEPTKIICAAKNYPWGENPSKSPLPILFFKPPSSVIGHEENIVYPSSSQHLI
                     FESELVVVMGKKARNIPAEDALRHVLGYTCGNDVTAYDFILRDNATFHGKSFDTFCPL
                     GPFIETDLEPDDTTITCRVNGKVQVSGSTKNKYYSCAELISFISEIMTLYPGDIIMTG
                     TPNIGTINRGDIVEVDIDGIGVLKNRVV"
     gene            8545..9426
                     /locus_tag="VEL68_17770"
     CDS             8545..9426
                     /locus_tag="VEL68_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ37876.1"
                     /translation="MRRQDFSEEFLVYGHGACPGCAMPVVTRNTFHALGPDTISVITA
                     SCLGTILCTYPFSPIKISTYATPFASAASAASGIKAALDMRGDFHTTVVAIAGDGGTF
                     DIGIQALSAAAERDEDFIYLCYDNEAYMNTGIQRSSATPWGAWTTTTPPESLKNQPKK
                     DIVEIMAAHRVPYAATASIAYPEDFLRKIKRAKETRGFKFIYALSPCPVGWRFSSENT
                     IRLSRLAVQSKVFPLYEVENGDRYILNVEPDGIPVKEYLQLQGRFSFLREGDMERIQG
                     AVDHEWDRLMRRIEVGC"
     gene            9916..>10103
                     /locus_tag="VEL68_17775"
     CDS             9916..>10103
                     /locus_tag="VEL68_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37877.1"
                     /translation="MKRCWILILGVVLLATVFPAYAQEGSKENVKELLKAVVKVRAVV
                     PDYARSAKTLGTEREGSGV"
BASE COUNT         2338 a         2700 c         2754 g         2311 t
ORIGIN      
        1 gttgcttcct tcggtgggtt gccggaggat gttccggtgg tgggggactg gaccggggac
       61 ggcattgcca agctgggaat ctaccgccag ggccagtggt tcaaggacct gaacaacaac
      121 gggacctggg acggctgcgg agtggatgcc tgcatcgaat cctttggagg atttgccctc
      181 gatattcctt tcgtcaaata gccccctgat tcctaaaggt cgctgccccc gcggatgcga
      241 cgcctacttc cttgccccgt gcagggactc atttcccgtt tcccttttga ccactcgatt
      301 tgctcgcgat tcccaggatt cctaccttgg gatgctcgat tagatggtcc aagcgaagtc
      361 ccgcaccttt aatcgccgtc tttaaatcct cgctgcggaa gcgcctgtcg gcgggatgga
      421 ggaggatatg tttggtgatg aagttttggt ataaggaggg gtaaagctcc tctaggtaat
      481 agactccgcc gggtttaaga acccggacga tttccatcag cgcgctctcc caatccggca
      541 cgtgatgaag gacgccgaag ccaaagaccg cgtccaggga ttcaaccctg gccgggaccc
      601 gccggacgtc agccacatag aaagaaatct cctttctttc cccttcaggg agataagtct
      661 tgcctctgcg gagcatctcg gggtccaggt ccatggcctg gagcacggcg ggttggaatt
      721 ctttttggat gagggcagct cccacgcccc gaccgcatcc cacttccaga acaacgctcc
      781 ccggtgcgag aggcctccgt ctcttcatcc agcgaatctc caacctctgc tggataggac
      841 gggaagggtt attgaccgcc catcgttcag cccagttgag tttcatggta aagaagggaa
      901 ggtgaaagtg taagtgcaac tttcactttc ccttacccct tcactcccgt ataaataact
      961 gcgattccgt tcgtcagccg acgatgcatc acccgggtaa aaccgatctc tctgaggacc
     1021 cggctcaact cctccggagg aggaaatttg cggatggact cggggaggta aagataggct
     1081 tcccgggttc cagccaggat ctttcccgcc aggggcatga gatgaaagga atagaagtca
     1141 tagagccgtc gtaaggcggg ggaggttgga agggaaaact ctaggcacat gaatttcccc
     1201 ccgggcctga gcacccggtg catctccgcc agacccttat ccatgtgggt caagttgcgg
     1261 actccgaagc ccaccagagc cgcattgaaa accccctgag gacaggagat cccctcggca
     1321 tccccctgga tgaagcagac tctctcgctt aaagaagctc gagatacctt gggctttccc
     1381 ccctccatca tggcgcgatt gatgtcataa aggaaaaccc gccctctttc cccgaccctt
     1441 ctaccggcta ggatcgacag gtcagcagtt cctccacaaa catcgatgac tcggtctccc
     1501 ggattaagag acaaaccttc cacggcccaa cgcttccaga aataatgaag gccgaagctt
     1561 aggaccgtgt tcatgaaatc gtattttccg gcgatggagt tgaaatgccg gaggacctgc
     1621 cggcccttct ccctggctgg aattctccgg aagccaaaaa aggacatctc ttctggctgg
     1681 gcttgagggg ttgaactggg agtggagtgg tttttcgatt tcatagagga agcgataaaa
     1741 aattcttcat ctgtctccag aatataagaa ttcccgcccg gtttcaatgc tcggatcatc
     1801 agcgagggga gattttttta aagaattgta atggctaaag aaagctgatc ctgagttggg
     1861 gtgttataaa ggggagggga ccctgtttat tctcgggccc cctgtcattt ttggagaact
     1921 ccgatggtta ttgagaaaaa ggggtaattc ggtggagggc ggaaagagcg cgaactgcgc
     1981 tttccgggga tgatacggtg gacacacccg tgccacatag gtctctgatc tgctcaacgt
     2041 attctttata gcccagcaga acggctatga ccggcttatc tggaatcgtc tcccggtaaa
     2101 cgggccagtc gcccaacatg tcccgaagct tcacccccat ccggacccag atttcgatcg
     2161 ccgcgtaggg gataaccggg accaggataa agccatccgg gttggggtcg gagaatatgg
     2221 cccgacaggc gggacgaaag atgccgctgg gcccgatatc gaggggattc ttgatgttca
     2281 tccaagcggg agccttggct ttgatccttt ccgcggtatt taattcccag gagggaagcc
     2341 gcattccttc catgaaagct gcatcggcaa ccatggctcc catagacccg gaggtggtta
     2401 ggacgccgac ttgcttcccc ttcatcgggg gctgggtggt gaaaacccgg gcgatctcga
     2461 tcatctcctg aaggttttcg gcccggatgg ccccggcttg acggaaggcg gcgtcgaaga
     2521 cccgatcctc tccggagagg ctggcggtat gagaaagggt ggcttcccgc ccgaagggcg
     2581 tgcgcccgct tttcaggatc accacgggtt ttttcctgct ggtccggcgc agggattcca
     2641 aaaagccggc gccgtcccgg gtgccttcca tgtacaccag aatcacgcgg gtattcgggt
     2701 cggcggcaaa atattccaga aagtcggctt cattcaagtc gatcttgttt cccagggtgg
     2761 caaccttgct gattttcaac cccgccgctg gaaggtggtc gaggaggatg ttcccgaaga
     2821 ctccgctctg ggccataatg gctactggtc cgggttccat ggaggagtcc atgatttcga
     2881 tggtggcgaa gcggttttcc gtgttcacca cccccacaca gttcggcccg atcagtcgga
     2941 ttcccaaccc cctggccagc gcggcaatct cttcctgcct tttttggccc tcggggccga
     3001 tctcggcaaa gccggcactc tcgatgatga ccgcccgaat cccctttttc cccaactcga
     3061 ttaaaacctc ccgaaccgcc ggcgagggaa cgatcaccac ggccaaatca actccgccgg
     3121 gaaggtcgga gatggaaggg atgactcttt tcccgtgcat ctccccccct ttgggattca
     3181 ccagaaaagc cctgtccagc cagccgttct ttctccagaa caatgggatc ccgtgcccaa
     3241 acccgggatt gttggtggcc ccgataagag cgacagatcg gggaacaaaa aaagaatcca
     3301 ggatggaacc ctgcattcaa tctcctccga tgaaaagaat gcctctgctt ttttgcatgt
     3361 ctccataatt ttatagacaa agactcaggg aaatgtcaac tcggaagacc ggaacctcat
     3421 gctcctcaga accaaaaggg attgcccagt aagaaatcca cctttgtctg ccgcaagccc
     3481 tttgggatca tggacatcca ggctctgggc tataaagagg aaatgatcaa aaaactgacc
     3541 cagagttatt aaattcgatt ctcctttcgt ccctctatcc ggtgcctcca tcccgaaggg
     3601 ggatggggct cctctttccc ctatctaacc atcagaaagc atagaggaaa aattttggct
     3661 gacctttcgc tgacgaccgg agaaatggca agagatttgc atttttccat tctgattgat
     3721 tgggctcctt aactcggcaa ggcagaaagg cgatggccat gcgaaagaat tccatccggg
     3781 cgacctgcgt ctatttatta gcggcaacct ctatatccat cctatccgca ttccttgcag
     3841 gatgcgctga aattcaggtc cgtccccttc ctcctgcgtc gccgacggct aaattgcgga
     3901 ttttcgttct gccgctgaca gggtctcctc ctccgggggg atgggggaag cctcatggag
     3961 aatttgcccg aaaccaaatc cgggcaatca gaattatttt ggaggggaca ggaatctacg
     4021 aaatgcccgc aagggaggat tatctcagcg tcatcggcca gcaggaggtt tccaactggg
     4081 aatggaaaag gaaagactgg gctctggcta aacgggttgg acacgcactc tctgccaact
     4141 atgccttagt cattgagcgg gatttcaatg ccgggatcaa cttctatcag acattgctga
     4201 tgaatctgga aaccggaaaa cagttcaagg ttgcttttcg cgttcccaag ggccgggagc
     4261 acagggaaga atggcagcag attgggcggg tggcctatac cgaaattttt cgggatgcca
     4321 agaacgatat gctggcaacg gccatgcgca aaggccgggt gtcggtttcc gggattcccg
     4381 ctccgattga cagagggacc aaagaccccg tcggctccag gccagcagac ccttccaaag
     4441 aacggacaat tcttccacca gcgccaccca aagtacggga agtggctttg gaagaatcta
     4501 tgaaagaaat tccggcgtcc agtagtatcc gtctggccat ttatgatatt cacgccaacg
     4561 agcccctcaa gattgccgcc ttgattctct ccgaagctct gcgggaggag ttcttccgtt
     4621 tgggaagatt tatgctggtg aaccgggaga acatggccca gatcctgaac gagatggggg
     4681 tgcagcagac ggggctggtg gaggagaaac aggccgtaca agcaggaaag gggctggcgg
     4741 cccgtcagat tattttggga caattcggct ccatcggaaa tacccttctc ctccaggtca
     4801 aaaggatcga tgtggagact cagtccaccc tttccatcgg ctccgctaaa tgtactccgg
     4861 ggaaggaaga tgaggtactg gcaggcctgc cagaactcgc ccgaaggttg gcagggaaat
     4921 aaggattaca ggccgcggag aaactcggga cgcaacaggg ctttatcttt cgcggataca
     4981 accttacgga tcagattgac caccgcctct ttatccttgg gcatccgcgc tttgatgggc
     5041 aggtaattgg aagcatgatt ggaggtgaag tagcagcggc tgaagtttga gccggctacg
     5101 atcgttccca actcttcaag catttcgaag ggagagggaa gctggaaacg tcccgctttc
     5161 atctcttcat ataaaggggt tcccggaacg accataacgg tcagggcgct ggcaaaatct
     5221 ggatcgatgt cggagagcac cttggctgtg gcttcagcgt gctgccggcc tccctgcgcg
     5281 ccggccagtc ccagcaggac cgtcgtcgaa agactgattc ccgcctcctt cacccttcta
     5341 ccagcttcta ccatttggct gtaagtggcg cctttgcgga ccttctccag aagggccggg
     5401 tctcccgtct ccactcccag ataaatgatt ccgagcttgc gatccctcag ctccttgagg
     5461 tcttccacgc tcttgcgcag gatgctcttg gcatttccgt aaatccccac ccggcgcagc
     5521 ccggcgaaca tttggttcag ataatcgaag atggcgatga gcttcggctg gggaatgatc
     5581 agggcgtccc cgtcagccag aaagaccttc cggaagggtc cataaccggc catttcatcg
     5641 atctcttcct tgatctgatc caaatcctta atgcggaatc tgcggccctt gtaagtagga
     5701 cagaaggtgc atttattgtg ggaacacccc agagtaacct ggaggatgag actttcggct
     5761 tcgctgggcg gccggatgac gatgccctca tattgcataa tcatatttta cctttgctta
     5821 cctccgttga caagcgagga acgaaaatga ttccattctt cattgcggag ggacataaat
     5881 ggacaggaga gctgatcctt tttccctgac ccgggattgt tcacattcct ttatgaggga
     5941 aaaaccgttt gtctgctgcg gccaataagg gctgatacag agtgattttc gttatccctg
     6001 ggcaagggga agaaataggg gggaatgctg gtctggtggc attaaaattg cagtccatct
     6061 aatgggatta aagaaacttt ctattgcatt ttcttgaatg caaaccccct ggctctgtcg
     6121 gcggtcatga ttccgactca aggaaagggc agcgatgaag aaagggcctt ttcatttcgg
     6181 cctggttctg ggtttgatgg ctttgggagg gtgcgccgga agttctggaa agacggttgc
     6241 ccgacagacc caacaaggcg cagaattccg gcccaaaagg atttatgcgg tggaaatgaa
     6301 acaggcctgg gatttgaccc tcaaagcttt gaaacgggaa gggatccccc tggagatagt
     6361 caacaaggag atcggggtaa tccggaccga ttatcagaac ctttctccgt gggaaagaag
     6421 taaatgcgat atccgatttt cccaggaacc cgaacagaat acccatattt acgtcctatg
     6481 tcggtatgag ggtaggaagg acatcagcga acccttccgg gatttcacct acagcttacc
     6541 ccggcaggcg atgaaggccg aagaggaaat ctacagaaag ctcgagcctc acatcctttc
     6601 ctatgaacga attccccctc gccaggaaga aacctcggtc aaagccgcaa tcccttccgc
     6661 ccctgtacca tattcgaaaa atccaggtcc aagtgcaaag acagcgatgc cgtcaaaccc
     6721 cccggcggcg gtcatttctc cggcagcagc gcaagccaaa atcgagaccg tttccccgac
     6781 aacttcgccg gtggccgaag ccgagaaacc ccagcctgcg gtaggggctg ccccacctcc
     6841 taggtccaaa ggagaggcgg ctacgctcac tgccgcagtt ctcccaaaga aagccgaaat
     6901 caaagaagag agcctatccg ctcccttgat gaccgtagct cctactaacg tgcgggtgga
     6961 tccctcgacc cagagcaaaa tcgctgctgt cctgaagaag ggggagcagg ttgaaaaaac
     7021 cggggaatcg ggaagctgga ccagaatcag gctatcttcc ggagagaatg cgtgggtctt
     7081 caccgacttt ttacagccgg ctacttccga gccctctcta acgtctcctg caccggcagt
     7141 tcagccttct cttcaaacca aacccggaca ggcccgacag attcttttag ccaaaccagt
     7201 agaaccggac aagaagggaa ttgaaggggg ggctaaaatc attttcatga cccgagatat
     7261 taccaaaatg tgggctgaac ccaactcccg aagtgaggta gtcctggtcc tgaaaaaggg
     7321 caggaaggta gaaaggctca ccgaatccgg tgaattcacc aaaatcaggt tatcctgggg
     7381 gagtagcgga tgggtattga cccgtttttt gcagacggtg ccctaggatc gctgtgattt
     7441 cagcagtccg aagatttcct tcttttgcac ttatgaaaat cgttcaatgc gaatatcccc
     7501 gaggcatttt cctggcttct ggagcccgac cgttaacagc tgatcgctga aagctgacag
     7561 ctatttttat accacccgat tcttcaatac cccgatcccg tcaatatcca cctcgacgat
     7621 atcgccacgg ttgatcgtgc cgatgttggg ggtgccggtc atgatgatgt ctcctgggta
     7681 tagggtcatg atctcggaga tgaagctgat cagctcggcg cagctgtaat acttgttttt
     7741 tgtcgatccg ctgacctgca ccttcccgtt tacccggcag gtaatcgtgg tgtcgtccgg
     7801 ttccagatcg gtttcgataa aggggcccag ggggcagaag gtatcgaagc tctttccgtg
     7861 gaaagtggcg ttgtcccgca agatgaagtc ataagcggtc acgtcgttgc cgcaagtata
     7921 ccccagaaca tgccggagtg cgtcctctgc aggaatgttc cgggcctttt tccccatgac
     7981 cacgaccaat tccgattcaa agatgaggtg ctgggaagag gaaggataga cgatattctc
     8041 ttcgtgcccg atgaccgagc tggggggttt gaagaaaaga atcggaaggg gagattttga
     8101 gggattttcc ccccatggat agttcttggc ggcgcagatg atcttggtag gctcgcaggg
     8161 ggggaggact ttcactgctg gtagaggccc gaccgccttt cctttgacgt tcggaccctc
     8221 gaatgaagac ccgagccggt aaaccgtctc cccttccaac attccgtaat gaacctggtt
     8281 atccttaagg taacgaagtc gtttcatcgt tcctccttta ctttccagtt taaatcccct
     8341 cctgatggaa aggttggaga ttcttgacaa aatatcctac catccggcaa ggctcggcgc
     8401 aatccgaaat caccccagtg caccccagtg cagaataggg agattatgat tttgccattc
     8461 tggattgggt gttggaagga tcggcttgat ttgctctttg acctcagata tgattcaaaa
     8521 gagattcgag gaggggttgc aggaatgaga aggcaagatt tctctgagga attcctggtt
     8581 tatggccatg gcgcctgccc cggatgcgcc atgcccgtag tcacacgaaa tacctttcac
     8641 gccctggggc cagatacaat ctcggtgatc accgcgagct gcctcggtac gattctctgc
     8701 acctacccct tttccccgat caaaatcagc acctatgcga ctccattcgc atcggcggcc
     8761 tcggcagcct ccgggataaa ggccgccctg gatatgcgcg gagatttcca tacgactgtg
     8821 gtggcaattg ccggcgacgg gggaactttc gacatcggca ttcaggccct ttccgcggca
     8881 gccgagaggg acgaagattt catctatctt tgctacgata atgaggccta tatgaatacg
     8941 gggatccagc gctcctcggc cactccctgg ggagcctgga ccactacgac cccccccgag
     9001 agcttgaaaa atcagcccaa aaaggacatc gtggaaatca tggccgccca ccgggtgccc
     9061 tatgcagcga ctgcttctat tgcctacccc gaggatttcc tgcggaaaat caagagggcc
     9121 aaagaaaccc gggggttcaa attcatttat gcgctctctc cttgccctgt ggggtggagg
     9181 ttttcctcgg aaaataccat ccgactttcc cgtctggcgg ttcagagcaa ggtcttcccc
     9241 ttgtatgaag tggagaatgg ggatcgttac attctcaatg tggagcctga tgggattccg
     9301 gtgaaggagt acctccagct tcaggggaga ttctctttct tgcgggaagg ggatatggag
     9361 cgaatccagg gggcggtgga ccatgaatgg gatcgtttga tgagacggat agaggtgggc
     9421 tgttgaagaa aatagggcct ttcctttttg gatgtattat tttgctgatg gcggtccagg
     9481 cttatgcagt tttttatgat cccccggttt gcgccattcc cgtccccctc ggccaggggg
     9541 aagggccttt catcggagaa atcttgattc aggttcctga acctggcgtc ctgttattgc
     9601 tgggagcagg tctcattttg ctcgtcatct tgggtattag gaaaaggaaa agaacagctg
     9661 cgaaagggaa gaacaatccc ctatagaccc agatgcccgc ggattccatc tttacgattc
     9721 tcagaacaga agtgccattt cctgttttaa ccgaagcccc tccttcctcg ttgtaactat
     9781 cccttttgac tttcggaaga aagttttata ataaccgaga gcccccgcgt cctctctgcc
     9841 agaaaaaagg atgtccaaaa ttttcgagca gtcttctgga tcagtagaac ccgcacctgt
     9901 ccttcggagg tatccatgaa aagatgctgg atcttgattc tcggggtggt cttgctggcc
     9961 acggttttcc ccgcctacgc ccaggagggc tcgaaggaga atgtcaaaga gcttctcaaa
    10021 gcggtggtga aggtccgtgc cgtggttccg gattatgccc gttcggccaa gactctcggg
    10081 accgaaagag aagggagcgg ggt
//
LOCUS       DASPRR010000483         6056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_263603, whole genome shotgun sequence.
ACCESSION   DASPRR010000483 DASPRR010000000
VERSION     DASPRR010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263603"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VEL68_17780"
     CDS             complement(<1..1084)
                     /locus_tag="VEL68_17780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ37878.1"
                     /translation="MQLNVPFMLLRNVHENPDKLAVVFGDKRYTYRTFNERVNRLANA
                     LLNHKIQKGDKVAYLMNNCSEFAEVSFALSKIGVLSVPLNFRLKDDEIGYVIQNSDSS
                     FIFFGSEFKKTIAGLIPRLPLVKKTIQVSGSAEYEDLLRSSSGEEPPVQVLEEDEHSI
                     MYTSGTTGFPKGAAHTHRSRVWNSLNLLVDTGLQGSDVFAITSPMFHIAAGHTMLFSP
                     IFIGATVVILPSFSLPEFFGVIQKEKVSAFFAVPTMFVRMVEHPNFKDYDLSSVRLLF
                     TGGAVTSVELKEKIMKAFPRATLDDLMGLTEGGPLTTFLPHRDALRKPGSVGRAHFSQ
                     MVRVVNDRGKDVHGEEVGEIIVKGPAV"
     gene            complement(1158..1955)
                     /locus_tag="VEL68_17785"
     CDS             complement(1158..1955)
                     /locus_tag="VEL68_17785"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXZ37879.1"
                     /translation="MLAKDMRRLIIKGKVYDLGQPYHPGMPHHPNHPPFAFTLTKKHG
                     DMVYPGGVSSANCLFTTGGHTGTHLDSLGHISLEGVLYGKLKAEKVQDYSTGIQLRGI
                     HTTPPVVRRGVLLDIAGALGKKALPHAFPIGPEELEKAAKKEKVSIKAGDAVLVRTGW
                     ARYWKDPMKFVAHEKGAPGVNLGGAKWLARHKIAFTGSDTTAYEKTPTQDLPVHVYLL
                     AQNGIQLMEMLTLEDLAKDRVYEFLFVALPLKIIGGTGSPIRPIAIH"
     gene            complement(2057..3211)
                     /locus_tag="VEL68_17790"
     CDS             complement(2057..3211)
                     /locus_tag="VEL68_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ37880.1"
                     /translation="MDFELPAELKKIQEETRQFVDREIRPLVPDIEKRKKNPIEIVRR
                     MAKQGVYRFLVPKEYGGMYERVRSLPICVIREELGRGHNFAAASIATNGLGSYPLVLA
                     GSPEMRNRFLPKVARGEVIPAFALTEPEAGSDPSGMTTIASKEGDSYRINGTKCFISN
                     AGIAHYIILFCKTDPALGTKGISAILVETGTPGYTVSRQMEILAIDVVNELQFKDCLV
                     PRKNLLGEEGKGFVVAMQTLDLLRCSVGAHAVGVAQEAFDLALAYAKKRVQFGKPIAQ
                     HQAIQMKLANMAVEINCGRLLVYQAALAKDSGQPDVTLKSSMAKYYCTEMAQRVVDHA
                     VQIHGGYGVLMDDFSMERLYREVRAPRIYEGTSEIQQIIIANHFVREKKK"
     gene            complement(3450..4544)
                     /locus_tag="VEL68_17795"
     CDS             complement(3450..4544)
                     /locus_tag="VEL68_17795"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXZ37881.1"
                     /translation="MKITRLEAIPFRIPYKTPLKWGLSGYLEAAEHVLVKIHTDAGVT
                     GVAEATPRPTIYGESQASILYAIRNWFAPMVVGMDPGHTEKVWAKFEGIHWNPTAKGA
                     IDLALYDAVARARGVPLWEMLGGNSDRLPVSWMLGIRSVSEMVQEALEMRARGFKAFK
                     VKVGIDSEKDVQVIKALRENLGSDVLLYADANMAYSVSTAVRTLRRMEEFGLAFVEEP
                     LPVWDQKGRLKVSRSISIPIMGDESVFTPQEVAREIELGAIGIISIKTPRTGYTLSLK
                     IINMAETAGIPCLMGTQAETGAGTLASAHFGAARRNVSYPSEISFFLSLKDDLLAEPI
                     SLQDGMIELPRRPGNGAILDEEKLKRYRMD"
     gene            complement(4763..5971)
                     /locus_tag="VEL68_17800"
     CDS             complement(4763..5971)
                     /locus_tag="VEL68_17800"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ37882.1"
                     /translation="MDEKKSNPLSGLRVLELATNVAGPLGATLFAEFGAEVIKVEIPG
                     TGDPARHFAPFYKEKSLTYAVLGRNKKSVTLDLRKPRGQELLKRLVKISDVLTENFRP
                     GTMEKWGLGYEEIKKVNPRIIMVRVSGFGQDGPYKDRAAFDRIASAMGGLTYLTGYPE
                     SPPVRVGLNLCDEIAGLFSAFGTMVALYHRDVSDRGKGQWIDVSLCESIIRLLEGVIA
                     EYGKLGKIRERSGNLNEVVAPADNFLSRDGKWIVFVVTSDALFQRFAMTIGRGDLAQD
                     PRLLKNLDRLKNRDALHEIIRPWFQEKTVSEIDKIFGENGVPYGLVYNAKDICEDPQN
                     QFRENIVEVQDPEIGPVQMQSVTPRLSETPGKVRSSSPGLGEHNQEVWGELLGLTEEE
                     LEDLGKEGVI"
BASE COUNT         1276 a         1691 c         1588 g         1501 t
ORIGIN      
        1 taaccgcggg gcccttgacg atgatctccc ccacctcctc gccgtgtacg tcttttcctc
       61 gatcgtttac cactcggacc atctggctga aatgggcccg acccacggac ccgggcttgc
      121 gcaaagcatc ccgatgcggc aggaaggtgg tcagcggccc cccttcggtc aaccccatca
      181 ggtcgtccag ggtggcacgg ggaaaagcct tcatgatctt ctctttcaat tcaacggagg
      241 tgacggctcc ccctgtgaac aggaggcgga ccgagctcag gtcataatcc ttgaaattcg
      301 ggtgctccac catgcggacg aacatggtgg ggacagcgaa gaaggcgctt accttttcct
      361 tctggattac cccgaagaat tcgggcaggg aaaaactcgg gaggatcact accgtcgccc
      421 cgatgaagat gggggaaaaa agcatggtat gcccggcggc gatatgaaac atggggctgg
      481 tgatggcgaa cacatcgctt ccctgaaggc ccgtgtcaac cagcaggttc aggctgttcc
      541 agacccggct ccggtgggta tgcgctgctc ctttgggaaa acccgtggtg cctgaggtgt
      601 acatgatgga gtgctcgtcc tcttccaaca cctggaccgg cggttcctct cccgaggacg
      661 atcggagaag gtcttcatat tccgcggacc cgctcacctg gattgtcttt ttgaccaggg
      721 gcagccgggg aatcaatcca gcgatggtct ttttgaattc cgacccgaaa aaaatgaagg
      781 aagaatccga attctggatg acatatccga tctcgtcatc cttgagccga aagttgaggg
      841 gaaccgagag gaccccgatc ttgcttaagg cgaaggagac ctcggcaaat tcgctgcagt
      901 tgttcatcag gtaggcgacc ttgtcccctt tttggatttt atgattcaag agggcattgg
      961 ccaggcggtt cacccgctca ttgaaggtcc tataggtgta ccgcttgtcc ccgaagacaa
     1021 ccgcaagctt atcggggttc tcatggacat tccgcaggag catgaaggga acattcaatt
     1081 gcatggccga ttataccaag atggcagatg ctggatgctt gatccaggac ccagtatccg
     1141 atatccagta tctgaattta gtgtatggcg ataggacgga tgggcgatcc ggttccgccg
     1201 atgatcttca ggggcaaagc cacaaacagg aattcgtata ccctgtcttt ggccaggtct
     1261 tccagggtga gcatctccat cagctgaatt ccgttttggg ccaggagata tacatgcacc
     1321 ggaaggtcct gggtcggcgt tttttcatag gcggtcgtgt ccgaacccgt aaaggcaatc
     1381 ttatgccggg ccagccattt ggcccctccc aggttgactc ccggcgcgcc cttttcatgg
     1441 gccacgaatt tcatagggtc tttccagtac cgggcccagc ctgtccggac aagcacggca
     1501 tcgcccgcct tgatggaaac tttctctttc ttggcggcct tctccaactc ctcaggtccg
     1561 attggaaagg catgggggag tgctttcttg ccaagagcgc cggctatgtc caacaaaacc
     1621 cctcggcgca cgaccggggg agtggtatgg atccccctta gctgaatgcc ggtgctatag
     1681 tcctgcacct tctcggcttt caattttccg tagaggactc cctcaagaga aatatgtccc
     1741 agagaatcca ggtgcgtgcc cgtatgcccg ccggtggtga aaaggcagtt ggctgaactc
     1801 actccgccgg ggtacaccat gtctccatgc ttcttggtca gggtgaaggc aaagggaggg
     1861 tggttgggat gatggggcat gccaggatgg tagggctgtc ccaggtcgta gacttttcct
     1921 ttgatgatca atcttctcat gtctttggca agcataagac ctcccgggaa gaagtgttag
     1981 tgctagtgtc agtgtcaatg tttagtgtca gcgaaaaacg cgaattcatt ttctgtcact
     2041 gacacttgca ctgacactat ttctttttct cgcgcacgaa atgattagcg atgatgatct
     2101 gctggatctc ggaagtccct tcatagatcc ggggggcgcg cacttcgcgg taaagccgct
     2161 ccatggagaa gtcgtccatc agcactccgt atcctccatg gatttggacg gcatgatcga
     2221 ccactctctg ggccatctcg gtgcagtagt atttggccat ggaagatttc aaagttacat
     2281 cgggctggcc gctgtccttg gctagggcgg cctggtagac caggagcctg ccgcagttga
     2341 tctccacggc catgttggcc agtttcatct gaatcgcctg gtgctgggca atgggtttgc
     2401 cgaactggac ccgctttttg gcataagcca gggccaggtc aaaagcctcc tgggcgacgc
     2461 ccacggcgtg ggcgcccacg gaacagcgca aaaggtccag ggtctgcatg gccaccacaa
     2521 agcctttccc ctcttccccc aggagattct tccggggaac caggcaatct ttgaactgca
     2581 gctcattgac cacgtcgatc gccaggattt ccatctgcct gctgacggta taacccgggg
     2641 tcccggtctc caccaggatg gcggaaatcc ccttcgttcc cagggcgggg tcggtcttac
     2701 agaagaggat aatataatgc gcaatcccgg cgttggagat aaagcacttg gtcccgttga
     2761 tccggtagga gtctccttcc ttcgaagcaa tggtggtcat ccccgatggg tcggatccgg
     2821 cctcgggttc ggtcagggca aaggccggga tcacctcgcc cctggccacc ttgggtagga
     2881 aacgattgcg catttctggg gacccggcca ggaccagggg gtagctcccc agtccattgg
     2941 tggcaatact cgccgccgca aagttgtgcc cccgtccgag ttcttccctt attacacaga
     3001 tcggaaggga gcggactctt tcatacattc cgccgtattc cttggggacg agaaaacggt
     3061 acactccctg tttggccatg cggcgcacga tctcgatggg attcttcttc cttttctcga
     3121 tatccggaac cagggggcgt atctcccgat ccacgaactg gcgggtttcc tcttggattt
     3181 tcttcaattc cgcaggaagt tcaaagtcca tatcttttcc ctcttaaata attgttttcc
     3241 caaggatggg atgcaaagtc gaaatccata aactttgtca ttccggcgaa agccggaatc
     3301 tagctatttc aaaatgttct ggaccccggc tttcgccggg gtgacaattc aaaagacttt
     3361 ttacgagacc actaagtagc tctatttcgt attttggatt ttagtctttt gaatatgttt
     3421 tccagttccg atattcgaat tttattcaat cagtccatcc tgtacctctt taacttctct
     3481 tcatccaata tcgcaccgtt tcctgggcgc cggggaagct cgatcatccc atcctggagg
     3541 ctgatcggct ccgccaggag atcgtcttta agggataaga agaaggaaat ctcgctggga
     3601 taggagacat tacgccgcgc cgcgccgaaa tgggcgctgg ccagggttcc tgcgccggtt
     3661 tccgcctggg ttcccatgag gcaggggatg ccggccgttt cagccatgtt aatgatcttg
     3721 agggaaagag tataaccggt gcggggagtc ttgatgctga tgattccgat ggctcccagt
     3781 tcgatttccc gggccacttc ctgaggggtg aatacgcttt catctcccat gataggaatg
     3841 gagatggacc gagatacttt taaccttccc ttctggtccc aaaccggtag cggctcctct
     3901 acgaaagcca ggccgaattc ttccatccgc ctgagggtcc ggacggcggt ggaaacggag
     3961 taggccatgt tggcgtccgc atacaggaga acgtcagacc ccaggttctc ccgcagggct
     4021 ttgatgacct ggacgtcttt ctccgaatcg attcccactt tcactttgaa agctttgaat
     4081 ccccttgccc gcatttctag ggcttcctgc accatctcgg aaacggagcg tatccccagc
     4141 atccagctta ccgggaggcg gtcggaattc ccccccagca tctcccagag gggaaccccc
     4201 cgggccctgg ccacggcatc atacagagca aggtcaatgg cgcctttggc tgtaggattc
     4261 cagtggattc cctcgaattt agcccagacc ttctccgtat gacccgggtc catgcccacg
     4321 accatggggg cgaaccagtt tcggatggca taaaggatgg aagcctggga ttccccgtat
     4381 atcgtagggc ggggcgtggc ctcggccaca ccggtcaccc ctgcatcggt gtggattttg
     4441 accaggacat gttccgccgc ttccaggtat cccgaaagcc cccacttcag gggagttttg
     4501 tagggaatcc gaaagggtat cgcttccaaa cgggtgattt tcaccaacac ctccgatgag
     4561 gggtttttaa gctggatgtc cgtcgttcgc tatctgcact tacaacccat gacctattac
     4621 cttggccttt accccccgtt taaaaagtat ttccacaatt cccttttatc tactcttttt
     4681 tgaaaatatc cggcgtccga tttcgctgga ttttattctc cccaaccttc aatgtcaaga
     4741 cgggaatagg ataaaaaatg acctatataa ccccctcttt cccgaggtcc tccagttcct
     4801 cttccgtcag ccccaaaagt tctccccaga cctcttggtt atgttcccca agccccgggg
     4861 agctggacct tacttttccg ggagtttcgg agagtctcgg ggttacgctc tgcatttgaa
     4921 cgggcccgat ttccgggtct tgaacctcca cgatattttc ccgaaattga ttctgggggt
     4981 cttcgcaaat gtccttggcg ttgtacacca acccgtaagg aacgccattt tcgccgaaga
     5041 ttttatcgat ttcggaaacg gttttttcct gaaaccaggg acggatgatt tcgtgaaggg
     5101 catccctgtt ttttagccgg tcgaggttct tcaaaagacg agggtcctga gcaaggtctc
     5161 cccgtccgat ggtcatcgca aatcgctgga aaagtgcgtc actcgtaact acgaatacga
     5221 tccattttcc atcccggctt agaaaattat ccgcgggagc aaccacttca tttaaatttc
     5281 ccgatctctc gcgaatcttc ccgagcttgc cgtactcggc gatgacccct tccagcaagc
     5341 ggatgatgga ttcacaaagg ctgacgtcga tccattgtcc tttcccacga tcgcttacat
     5401 cccggtggta taaagccacc atcgttccga acgcgctgaa gagacccgcg atctcatcac
     5461 agagattgag gccgacgcgg accggaggag attcagggta tccggtcagg tacgtcaatc
     5521 cccccatggc gctggcaatt cggtcaaacg ccgcccgatc cttatagggc ccatcctgcc
     5581 cgaagccgct cacccggacc atgattattc tggggttaac cttcttaatt tcttcgtacc
     5641 ccagccccca tttttccatc gtgccgggtc tgaaattttc ggtcaaaaca tccgatattt
     5701 tcaccaacct cttcagcagt tcctgccccc ggggttttct taaatcgagg gtcacggatt
     5761 tcttgtttcg tccgaggaca gcataggtca acgacttttc cttataaaaa ggagcgaaat
     5821 gccgggccgg gtccccggtc ccaggtattt ctactttgat cacctcggcc ccgaattccg
     5881 caaataaggt tgctcccaag gggcccgcta catttgttgc cagttccaga actctcagtc
     5941 cggataaggg gtttgatttt ttctcatcca tgttgattcg tataaggtct ttatccgatc
     6001 atgggctttt ggcggagttt gtactattaa gggttcccct agcggaatgg gaaaat
//
LOCUS       DASPRR010000484         9499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2211467, whole genome shotgun sequence.
ACCESSION   DASPRR010000484 DASPRR010000000
VERSION     DASPRR010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211467"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VEL68_17805"
     CDS             complement(<1..370)
                     /locus_tag="VEL68_17805"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ37883.1"
                     /translation="MGPTMALAEFITRLKFEDIPSKTIEQVKAAFLDSVGCGLFGSTL
                     EWSQKVNSLVKEMGGKEEATLWAHGFRGPAANVALGLGTMIHSFDFDDYHNAKLHPGA
                     AVVPAALTLAEREGISGKDFL"
     gene            complement(446..1327)
                     /locus_tag="VEL68_17810"
     CDS             complement(446..1327)
                     /locus_tag="VEL68_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXZ37884.1"
                     /translation="MTSFPSPGAVLRKILQEPEILVAPGAHNAFTARIIEQTGFRAVY
                     LTGSGASMDLLGMPDLGFLTMTEMVAHAGNIVQATRLPVIADADTGYGNALNVMRTIR
                     EYERTGVAGVHIEDQVAPKRCGHFAGKEVIPREEMIGKIKAALDARRDPDFLIIARTD
                     ARAVLGLEEAIERGKAYRQAGADTIFVDAPESVEELRLIPRSIPGPLMVNMSEGGKTP
                     LISARELQEMGYRLVIYPRSAAGAAAKAIQELLAVLKKEGTTKNCLDRILSFEGRNQI
                     TGLAHYQELEKKYLRKD"
     gene            complement(1431..3401)
                     /locus_tag="VEL68_17815"
     CDS             complement(1431..3401)
                     /locus_tag="VEL68_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HXZ37885.1"
                     /translation="MNNKVDQKAKAILEKEDEKASQRQMSGRVGQIITILAISMSLFH
                     LYTAGYMVYTAMVQRSIHLCFALALIFLMYPGGSRSPRDRVPLTDWALLLISVISCLY
                     ITINWEDLSEAVRIADPNTVDMALGILATLLVLEATRRTAGMAMPLVAIGFILYGFLG
                     PYMPGVLNHPGIPLNQFIGMNYLFSEGIFGVPLGVSATFVIIFIIFGGFLEVSGGGKF
                     FIDLACALFGAVRGGPAKIAIISSGFFGTISGSAVANVVGTGTFTIPMMKRIGYRPHF
                     AGAVEAVASTGGMIMPPVMGAAAFIMAEIVGVPYVSVCIAAALPAVLYYFSLFVFIDL
                     EAAKTGLKGIRKEEKPSIIQVFREGGHLLIPPIVLVYLLAFVQWSAIKAGFYSIVATV
                     LCAMARKSTRLDLKKLITALRKGATGALQIAAVCACAGIVISIVSITGLGLTFSSVLI
                     DLAGGNLFFLLVLTMIASLILGMGLPATPCYIILAVLSAPAIIQMGVSPMAAHLFVFY
                     FGCISAITPPVAVAAYAGAAIAQANPMRTGYTAWRLGLAAFIVPFMFIYGPPMIMVGP
                     ADEIVLAAVTSLIGVAFLAASIQGFALTHLGWVERVALFAAAVLLIKPGWITDLIGIV
                     IGIIIGLIHISKYRKERAPGKISEVVVPPIGI"
     gene            complement(3590..4549)
                     /locus_tag="VEL68_17820"
     CDS             complement(3590..4549)
                     /locus_tag="VEL68_17820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ37886.1"
                     /translation="MKNRIFIAVLLAAFLFTLTTSPGFAADKKYKFMLGTAGAGGEVY
                     IWGGGAAKVVSQFAKDVQLTAQLTAGSGENLIRLQKNTLKIGLASNEWNWELYNGKGG
                     LPKYEHRALLAMYKGDWHWGCRKTFPGNTIYDFKGKKVSFGPKGGGSYGMIKYVLDAL
                     GLKFTDFDAKYLSVAESVDALKDGVIEGYFVGIGTPSSAFMDLATMPSGLKLISLSPA
                     DIKKCNEKYNFLSETVIPANTYKGVDYESRGPGRWHFMTCRHDFPEEAAYQIVKALTE
                     HHKELVAVMTAAKLSTPENTIQYNILPLHPGAEKYFREKGYLK"
     gene            complement(4669..5082)
                     /locus_tag="VEL68_17825"
     CDS             complement(4669..5082)
                     /locus_tag="VEL68_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37887.1"
                     /translation="MKKIFCLATLAAIFLLGSSAGLLAAEKKHSISLETPPQAQVLQI
                     TSYLKFLKEFGEGKPAMHVEVKIKNLADKPERFSITVSTPDGDSAAAFIPEKAKKEGS
                     LPVLEPREEGKITLPLLVEKLVDSFTVTVELIPAE"
     gene            complement(5093..6280)
                     /locus_tag="VEL68_17830"
     CDS             complement(5093..6280)
                     /locus_tag="VEL68_17830"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ37888.1"
                     /translation="MNQPLSGVRILDLSQLLAGPYGSMILADLGAEVIKIEKPGEGEI
                     GRGMPPHFFHGESVYFISINRNKKSMTLDLKAPEGLEVFYRLVGVSDVVYDNFRPGTL
                     EKLRIDYESLRKINPRIISCSISGYGQTGPFKDSPAFDLIIQARGGIMSYTGEPGQMP
                     VRMGAPMGDLAGGMFAVQAVEAALYQREKTGQGQRIDLSLLDCQIALLIYRGLYYLYG
                     GEIAQPVGSGHVSAIPIRAFKTKTFDIVIDANTDKFFQELCGAMGLQELLSDPRFPSR
                     PYRLKNKEALYKILEETFLKKTGEEWLKLLEGRVPVGPINTVDMALNDPQILSRNMVV
                     EATHRTGEKMKLLGTPIKMSQAEEREFTPPPALGEHTDQVLADLLKFSPKEVQRLREK
                     KIV"
     gene            complement(6299..6718)
                     /locus_tag="VEL68_17835"
     CDS             complement(6299..6718)
                     /locus_tag="VEL68_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXZ37889.1"
                     /translation="MTDRRPIRVLIAKPGLDGHDVGAKVIVQRLRNAGMEVIYTGLRQ
                     STEKIISAAIQEDVDVLGLSILSGSHVELCQELMEGLRGKGIKDLLVIVGGIIPKEDI
                     AVLKSHGVDGVFPVHSTLEEIETFIREQVLRRRGELA"
     gene            complement(6893..8554)
                     /locus_tag="VEL68_17840"
     CDS             complement(6893..8554)
                     /locus_tag="VEL68_17840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXZ37890.1"
                     /translation="MGASDAAAKEKKAAGSTEKKKGPREAYTSFGEPVKVSYGPEDLA
                     GFDYSRDVGDPGAYPFTRGIYPTMYRSQLWTMRPYAGMATAEETNERFKFLLKSGQTG
                     LSLAFDLPTQLGMDSDDPLARYDVGKLGVAVDTLDDMERLFEGIPLDKMSTSFTINST
                     AAIILAMYVLVGRKQGVSPEALRGTVQNDILKEYVARGTWIFSPKPSMRLIADTIEYC
                     INHIPRFNPISVSATHLCEYGAKAEQSVSLPFLNALAYIDEVLKRGYSVDQVAPQIVF
                     HMAVGGRNFHLFEDVAKLRAARRLWARLMKERYGAKNPESMRLKFSTGALGGGMTAAE
                     PLNNITRGALFGLAAVLAGTRSLNMACFDEAYAIPSDLAIRTALRVQQILAYEIGVTD
                     TVDPLAGSYYVESLTNQMEERMRKTMADYEGRGGIVKAIEEGTLQRDLARQSYELQEK
                     VSRGEKVLVGVNRFTSKEEEKPIEVYRANPKTRDAQIARLNAVKAKRDSKKVQKNLAA
                     LRQAAENGENIFPALFPAVEERATVGEIISTLKQVFGEYQEPRTI"
     gene            8785..>9499
                     /locus_tag="VEL68_17845"
     CDS             8785..>9499
                     /locus_tag="VEL68_17845"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HXZ37891.1"
                     /translation="MKSAKWCSAREAMRQAIQPGDRIFFSIASGQPQTLLQALAEDFE
                     FYRDVEVINGILLAEHPLAKKGLQSSFRCISFQNSASVRQDWEEGRIDFLPVRYSDVP
                     RVFSRGGSKPLGAVLIQVSPPDSQGRFSLGVSASHAYPLALEARRIVAEVNDQAPRTG
                     GPCFFTEEQIDFLVECSAPLVPYREIQFGEAEKRIAEFVIDLIPDGATIQIGIGNLPA
                     AILKLLEQKKDLGIHSGMLS"
BASE COUNT         1978 a         2733 c         2589 g         2199 t
ORIGIN      
        1 tgagaaaatc ctttcctgaa attccctccc gctccgccag ggttaaggct gcgggcacga
       61 ccgctgcacc cgggtgcagc ttggcgttgt ggtagtcgtc aaaatcaaag ctgtggatca
      121 tggtccccag ccccagcgcc acgttggctg ccggtccacg aaaaccatgg gcccagagag
      181 tggcctcttc ctttcctccc atttccttca cgagggagtt caccttctgg ctccattcga
      241 gcgtggaccc gaatagcccg caccccacgg aatccaggaa agcggccttc acttgctcga
      301 tggtttttga aggtatatct tcgaatttta gccgggtaat aaactcggcc agagccatag
      361 tcggtcccat aaccgttcct cctaagttcg agattcgggt tgcattcttc atctagctta
      421 cacctctgcc tggcccgtat cggccttaat ccttccgcag gtactttttc tccagctcct
      481 gatagtgagc gaggccggta atctggttcc ttccctcaaa gctcaggatc cggtccaggc
      541 aattcttcgt cgttccctcc tttttcaaga cggccaagag ctcctggatg gccttggctg
      601 cagctccggc cgccgatcgg ggataaatga ccagacggta tcccatttcc tggagctccc
      661 gggcgctgat caggggggtc tttccccctt cgctcatgtt gaccatcaag ggaccgggga
      721 tggaccgggg aattaaccgc agttcttcca cagactctgg agcatccaca aagatcgtgt
      781 cggccccggc ctgccggtag gccttgcccc gctcgatcgc ttcttccagc cccaggaccg
      841 cccgggcatc ggtccgagca atgatcaaaa agtcagggtc ccttcgagca tccagggccg
      901 ctttgatctt cccgatcatc tcctcccggg ggatgacctc tttccccgca aaatgcccac
      961 accgtttcgg agccacttga tcttcgatat gcaccccggc aacgcctgtt ctctcatatt
     1021 ctctgattgt ccgcatgaca ttgagggcat tgccgtatcc cgtgtcggca tcggcgatca
     1081 ccggaaggcg ggtggcctgg acgatattac cggcgtgggc caccatctcg gtcatggtga
     1141 ggaaacccag gtccggcatg cccaataggt ccatggatgc gcccgaacca gtcaggtata
     1201 cggctctaaa tccggtctgc tcgatgatcc tggcggtaaa ggcgttgtgt gcgccggggg
     1261 cgactagaat ttctggttct tgaagaattt ttcggagaac tgcgcccgga cttgggaagg
     1321 aagtcatttt aatccctctt tctgacctgt ttcttacggt gccttcggga aatgcacccg
     1381 atatcgcttt ccggatattc tgtagggccc ggcctccggc caagccgttc tcatatgccg
     1441 ataggaggta cgacaacttc gctgattttg cccggggcgc gctcttttcg gtatttcgag
     1501 atgtggatca gcccgatgat gatcccgatc acgattccga ttaggtccgt gatccacccg
     1561 ggtttgatca ggagcacggc cgcagcgaag agcgcgactc tctccaccca accgagatgg
     1621 gtaagtgcaa aaccctggat ggaagcggcc aggaaagcca ccccgatcag tgaagtgact
     1681 gcggccagga cgatttcatc cgccggtcca accatgatca tgggcgggcc gtagatgaac
     1741 atgaagggga cgatgaaagc ggccagcccc aggcgccagg ccgtgtagcc tgtgcgcatg
     1801 gggtttgcct gggcaatcgc cgccccggcg tatgccgcca cggccaccgg aggggtaatg
     1861 gcggaaatgc aaccaaaata aaagacgaag aggtgggcgg ccatcgggga gacccccatc
     1921 tgtatgatgg ctggagcgga aaggactgcc aggatgatgt agcagggggt cgcggggagt
     1981 cccatgccca gtatgaggga agcaatcata gtgaggacga gaagaaagaa aagatttccc
     2041 ccggccagat cgatcaaaac cgaggagaag gtcaggccga gcccggttat ggagacaatg
     2101 ctgatgacaa ttccggcgca ggcgcaaacc gccgcgatct gcagagctcc ggtggctccc
     2161 ttgcggaggg cggtgatcaa tttcttcagg tctaaccgtg tgcttttccg cgccatcgcg
     2221 cagagcacgg tggccacgat ggaatagaaa ccggctttaa tcgccgacca ctggacgaaa
     2281 gccagtaaat ataccaggac gatgggaggg atgagcagat gtcccccctc ccgaaacact
     2341 tgaatgatcg agggcttctc ttctttgcga attcccttca gcccggtctt ggccgcctcc
     2401 aggtcaataa aaacaaaaag ggagaaataa tagagcacgg ccggaagagc ggcggcgata
     2461 caaacgctca cataggggac tcccacgatt tcggccatga taaaggcggc ggcgcccatg
     2521 accggaggca tgatcatgcc tccggtggag gccaccgctt ccaccgctcc ggcaaaatga
     2581 ggccggtaac caatccgctt catcatggga atcgtgaagg tcccggtgcc gaccacgttg
     2641 gccacggcgc tcccggagat ggttccgaag aatccgctgg agatgatggc gattttggcc
     2701 ggacccccgc gcaccgcacc gaaaagggcg caggccaagt cgatgaaaaa tttccctccc
     2761 ccggacacct ccaggaatcc cccgaaaata atgaaaatga tcacgaaggt ggccgagacc
     2821 ccgaggggaa ccccgaagat cccttcgctg aataaatagt tcattccgat gaactggttc
     2881 aggggaatac ccgggtgatt gagaaccccc ggcatgtaag gcccgaggaa tccgtagagg
     2941 atgaacccga tggctaccag aggcatggcc attcctgccg tccgccgggt cgcctccaaa
     3001 accagcaggg ttgcaaggat tcccaaggcc atgtctactg tgttgggatc cgcgatccgg
     3061 acggcctcgg agaggtcttc ccagttgatc gttatgtaaa ggcaggagat tacggagatc
     3121 aggaggaggg cccaatccgt caggggtacc cgatctctgg gagatcggct cccacccgga
     3181 tacatgagaa agatgagggc cagggcaaag cagagatgga tcgaccgctg caccatcgcc
     3241 gtgtagacca tgtatcctgc ggtgtagagg tggaagaggg acatggagat ggcgagaatg
     3301 gtgatgatct ggcccacccg cccgctcatc tggcgttgac tggctttttc atcctccttt
     3361 tccagtatgg cctttgcttt ttggtctacc ttattgttca ttgaccccct gcttcaagga
     3421 ttggttagaa atagatttcc ctcactctca ctactttctt tttgcccttc ccgcgcaggc
     3481 gggagccgag agctgcttga tgtaaccgga ttcctgcttt cgcgggaggg acctcttgat
     3541 gaaagtggag ttttcatcgc gcccagttca ccacaaagcc attgagcatc tatttcaaat
     3601 agcccttttc ccggaaatat ttttccgctc cggggtgaag gggaaggatg ttgtactgaa
     3661 tggtattttc cggggtggat aatttcgcgg cggtcatgac cgcgaccagt tctttgtggt
     3721 gttcggtgag ggctttgaca atctggtagg ccgcttcctc tgggaaatcg tgccggcagg
     3781 tcatgaaatg ccaccggcct gggccccggc tttcataatc gacccccttg taggtattcg
     3841 ccgggatcac ggtttcgctc agaaaattat atttctcgtt gcacttcttg atatcggcgg
     3901 gggaaaggga aatcagcttc agcccgctgg gcatggtggc caagtccatg aaagcagagg
     3961 aaggggtgcc gatccccacg aaatagcctt cgatcacccc atccttcagg gcgtccacac
     4021 tctccgccac agaaagatat ttggcgtcaa agtccgtgaa cttcaggcca agggcatcca
     4081 ggacgtattt gatcatcccg taggagcctc cccctttggg accaaaagaa accttctttc
     4141 ccttaaaatc atagatggtg ttccccggaa aggtcttccg gcatccccag tgccagtcgc
     4201 ctttgtacat ggccaagagg gctcgatgtt catatttggg caatccgccc ttgccattat
     4261 aaagttccca gttccactcg ttcgaagcga gcccgatttt aagcgtattt ttttgaaggc
     4321 ggatcaggtt ttctcccgac cccgcggtga gctgggcggt aagctggaca tccttcgcaa
     4381 actgactcac caccttggcc gctccccctc cccagatata aacctcccct ccagccccgg
     4441 ctgtgcccag cataaattta tactttttgt ccgcagcgaa accaggggaa gtggttaagg
     4501 tgaacaggaa tgcggcaagc agaacggcga tgaagattct gttcttcatt tcaatcccct
     4561 ttcttaaaag cagaaagatg ctgaagggtt ctatttctct gctttcgagt acccagaggt
     4621 tacagcgaat ggatagaaag acttgaaact cgaaggcttt ttctccgatc attccgccgg
     4681 gatcaattcc accgttacgg tgaaactgtc aaccaatttt tccaccaaca aaggaagcgt
     4741 gattttgcct tcttccctgg gttccagaac tgggagggac ccttcctttt tggctttctc
     4801 ggggatgaaa gcagcggccg aatctccgtc cggagtgctg accgtaatcg aaaaccgctc
     4861 gggtttatca gcaagattct tgattttcac ttccacatgc atcgccggtt ttccttcacc
     4921 gaactctttg aggaatttca aataggaagt tatctgcagg acctgggcct gcggcggtgt
     4981 ttccagggag atggagtgct tcttttccgc ggccaacagc ccagcactgc tccctaagag
     5041 aaagattgcc gccagagtcg ccagacaaaa tatttttttc atctttccgc tcctaaacaa
     5101 tctttttctc tcgcagcctt tggacctctt tcgggctgaa tttcagcaga tcagccaata
     5161 cctgatccgt gtgttccccc agggctgggg ggggcgtgaa ttctctctcc tcggcctgag
     5221 acatcttgat gggggttccc agaagtttca tcttctctcc ggtccggtgg gtggcctcca
     5281 ccaccatatt gcgggaaagg atttgcgggt cattcaaggc catatccacg gtattgatgg
     5341 gccccacggg aacacgccct tccagcagtt ttaaccactc ttcccctgtc tttttcaaga
     5401 aagtctcttc cagaatcttg tagagggctt ctttattctt aaggcgatag gggcggctag
     5461 ggaaacgggg gtccgagagc agctcctgaa gtcccatggc cccgcataat tcttgaaaaa
     5521 acttgtccgt attggcgtcg atgacgatat caaaagtctt ggtcttgaac gcccggatgg
     5581 ggatggccga tacatgcccg gaccccaccg gctgggcgat ttctcctcca taaagatagt
     5641 aaagaccccg ataaattaag agggcgatct ggcagtcgag taggctcagg tcaatgcgct
     5701 gcccctggcc ggttttctcc cgttggtaga gggcggcctc cactgcttga acggcaaaca
     5761 tccctccggc caggtcgccc atcggagcac ccatacggac gggcatctgg cccggctctc
     5821 cggtgtagct catgatgccg ccccgggcct ggatgatcaa gtcgaaggcc gggctatctt
     5881 tgaacgggcc ggtctgtcca tagccggaaa tagaacaaga gataatccgg gggttgattt
     5941 ttcttaggct ctcataatca atacgtaact tttccagggt tccaggtcga aagttgtcat
     6001 agacaacatc ggacacccca acaagtcgat agaagacctc cagcccttcg ggggccttga
     6061 ggtccaaggt catggatttc ttgttgcggt tgatgctgat gaaataaacg ctttccccgt
     6121 ggaaaaaatg cgggggcatc ccccgcccga tttctccttc accgggcttt tcaatcttga
     6181 taacctctgc tcccaagtca gccaggatca tcgatccgta aggcccagcc agaagttggg
     6241 aaaggtccaa tatgcgtacc ccggaaagtg gttgattcat ggtgccgatc ctttctcctc
     6301 aggccaattc ccccctcctg cggaggacct gctcacggat gaatgtttcg atctcctcca
     6361 gcgtggaatg gacggggaag actccgtcca ccccatgcga cttgagaacg gcgatatcct
     6421 ctttgggaat gatccccccg acgatgacca ggaggtcttt gattcctttg cccctcaacc
     6481 cctccatcag ctcctggcac agctccacgt gggacccgga taggatggag aggcctaaca
     6541 catccacgtc ctcctggatg gcggcggaga ttattttctc cgtgctctgc cgcaggccgg
     6601 tataaatgac ttccattccg gcattgcgta gcctctgcac gatcaccttt gcccctacgt
     6661 catggccgtc caaacccggc ttggcgatca aaactcggat gggtctgcga tctgtcatga
     6721 ataactcctt ctgcaatcat gacggattta tgaaaatttt attccctgct tatgcaaaac
     6781 ctctgaatct cgtacagaat ttgtaccttg caaacggttg tccgaaatcc cccccaaccc
     6841 cctttacaaa aggggggcga aggggggttt ttgctggttt agcccccttg agtcaaatgg
     6901 tcctcggctc ctggtattcc ccgaaaacct gtttcaaggt cgaaatgatt tctccaacag
     6961 tcgcccgttc ctccactgcc gggaagaggg cggggaagat gttctcgcca ttttcggcgg
     7021 cctgtcgaag ggcagcaagg tttttttgga cttttttgga atctctcttg gctttcaccg
     7081 cattcagcct ggcaatctgg gcatcccggg tcttcggatt ggcccggtac acctcgatgg
     7141 gcttttcttc ttccttggaa gtgaagcggt tgacccccac caggactttt tctcccctgg
     7201 agaccttctc ctgtagttca tagctctgtc gggccaggtc tctttgcagg gtcccttctt
     7261 cgatggcctt gacgatgcct ccccggcctt catagtcggc catggtcttg cgcatcctct
     7321 cttccatctg gttggtcagg gactccacat aataagaccc ggccaagggg tccaccgtat
     7381 ccgtgacccc gatctcgtag gccagaatct gctgcacccg cagggccgtg cggatggcta
     7441 aatcggaggg gatggcatag gcttcatcga agcaagccat gttcaaggac cgggtcccgg
     7501 ccaggaccgc ggcgagcccg aatagtgccc cccgggtgat gttgtttaaa ggttcggctg
     7561 cagtcatgcc tccccccagg gccccggtgg agaatttcag gcgcatggat tcgggattct
     7621 tcgccccata ccgctctttc atgagccggg cccagagccg ccgggcggcc cgcagtttgg
     7681 ccacatcctc gaagagatga aaatttcgtc cccccacggc catgtggaaa acgatctggg
     7741 gagccacttg gtcgaccgaa tatccgcgct tcagcacctc gtcgatgtaa gccagggcat
     7801 tgagaaaggg gagagagacg gattgctcgg ccttggcccc gtattcgcac agatgggtag
     7861 ccgacacgct gatggggttg aaccggggga tatgattaat acagtattcg atggtatcgg
     7921 cgatcaagcg catggagggc ttgggggaaa agatccaggt tccccgggcc acgtactctt
     7981 ttaggatgtc gttctgcacg gtcccccgca gggcctcggg ggatacgccc tgctttctcc
     8041 ccaccagaac gtacatggcc aggatgatgg ccgcggtgga gttgatggtg aaggaagtgc
     8101 tcatcttgtc cagcgggatc ccctcgaaaa gcctctccat gtcatccagc gtgtccacgg
     8161 ccacgcccag ctttcccacg tcgtaccgtg ccaggggatc gtccgaatcc atgcccagct
     8221 gggtgggaag gtcgaaggcc aggctcaacc ccgtctgccc gctcttgagc aggaacttga
     8281 accgctcgtt ggtctcttcc gccgtggcca tgccggcgta gggacgcatg gtccagagtt
     8341 ggctgcggta catggtaggg tagatgcccc gggtgaaagg gtaggcccct gggtctccca
     8401 cgtctcgcga atagtcaaaa ccggccaaat cctccggtcc gtaagaaacc ttgaccggtt
     8461 ccccgaaact ggtgtaagcc tcccgcggtc ctttcttctt ttccgtactc ccagccgcct
     8521 ttttctcctt ggcggcggca tccgaggcac ccatcgtctt tcctccttta ctccgcgagc
     8581 gtcagtcctc catctgcaac gctcggcaga aattcccacc cgtttcaatc agcaaaggaa
     8641 tatcttcttt tataatttta acagggtctt tgtcaacccg gttctctctc ggcagaaagt
     8701 tgttttcaat ttttggttgt gctaaattct gggtgtgtcc ttcaggcggg gctgattctt
     8761 tttgagggtt cgggaggaga agggatgaaa tcggcaaaat ggtgcagcgc tcgggaagcc
     8821 atgcgccagg ccatccagcc aggggaccgt atttttttct ccattgcctc cgggcaaccc
     8881 cagaccctgc tgcaagcctt ggctgaggat ttcgaatttt accgggatgt ggaggtcatc
     8941 aacggcattc tgctggccga gcaccccctg gccaaaaagg gcctccagtc ttctttccgg
     9001 tgcatcagtt tccagaacag cgcttccgtc cgccaagact gggaagaggg acggatcgat
     9061 tttcttcctg tccgttactc cgacgttccc cgggtctttt cccgcggggg ttcgaaaccc
     9121 ctcggtgcgg ttctgatcca ggtttccccc cctgacagcc agggccggtt cagcctgggg
     9181 gtttctgcga gtcatgccta tcccctggcc ctggaagccc ggaggatcgt cgccgaagtg
     9241 aacgaccagg cgccccgcac cggcgggcct tgttttttta ccgaggaaca aattgatttt
     9301 cttgtggagt gctccgcccc gctggtcccc taccgggaga tccaattcgg ggaggccgag
     9361 aaacggattg ccgaatttgt gatcgacctc atcccggacg gggccacgat tcagatcggc
     9421 atcgggaatc ttcctgcggc gatcctcaag cttctggaac agaagaaaga cctcggcatt
     9481 cattccggaa tgctatccg
//
LOCUS       DASPRR010000485         5608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_603551, whole genome shotgun sequence.
ACCESSION   DASPRR010000485 DASPRR010000000
VERSION     DASPRR010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603551"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VEL68_17850"
     CDS             <1..261
                     /locus_tag="VEL68_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37892.1"
                     /translation="SSHAALDPFGTRDEPFLFGLIMQGGQLGQVIGPPVFALMVSMGE
                     GGKSASWLLGVSAAAGVGFSLGLAVLKKRRLPIGPRNRPIVI"
     gene            complement(276..908)
                     /locus_tag="VEL68_17855"
     CDS             complement(276..908)
                     /locus_tag="VEL68_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HXZ37893.1"
                     /translation="MPIVTIRGPLGSGAPEIGKAIAEFLHADYVDREIIAGVAARLHR
                     QEHDVAAKEMPPSTLVGRIAEALDRGLPFGVGFEGTYLPTWEIPLDDKRYLQALESVI
                     KELARAPSLVIQGRGSQFILKNFPRTLHILTVAPFEVRLKRVREELKVGEEAAKQEMA
                     RFDNGSREFIKRYYHAGWEDPVHYDLSLNTERLSFKAAVSIVIQALSVKD"
     gene            complement(1037..1954)
                     /locus_tag="VEL68_17860"
     CDS             complement(1037..1954)
                     /locus_tag="VEL68_17860"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXZ37894.1"
                     /translation="MKRIGNLSNAGNELSGAKKILRHPYVLGMILLSLGIGLLPAVPS
                     AAQELKFGFTPVLSEADMKAEFEPLMAYLSNAVGQKVVLYVAKSYGDLRTQMEAGIVD
                     IGSFSPFAYVDAARGGKIRIIAQSILDGAATYRGLIVARKDSGLKTLDDLQGKRFAFV
                     DPKSASGYVYPRAMLIEKGFTPERYFRETIFAGGHDKVIAAVLEGRADAGAIYDGALG
                     VAKAMGMSTENLTTLASTDPIPHDAIAVRISMDEALSKKIQAALVDLDKSEAGRQAVA
                     KSKKKLTGHVIAEDSLFDVVRRTAKIAGM"
     gene            complement(2300..3295)
                     /locus_tag="VEL68_17865"
     CDS             complement(2300..3295)
                     /locus_tag="VEL68_17865"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXZ37895.1"
                     /translation="MYKVMVLTKKFSEFSREPFQVMKQAGLEVVEKDYDRASPAQEDE
                     LCQAIQGADAIIVTAMFPATRKFIMSADRLKMIAIRSAGFEGSDLKAATEKGVVVTNN
                     PGANAESVADMAIGLMLSVSKQITQKDREMRKGLYPRGSGSEDLFRKTVGIIGLGNIG
                     KRVAKRLQGFEAKVIANDIAEYPDFQKQYNIPYFSKEELLQRADFVTLHVPLDPATRR
                     MINEERLRLMKKTAFLINTARGAIVDEKALYKALKEGWIAGAGLDVFETEPPVFRDHI
                     LLDNVVSTPHSAGLSNQATHAMAMETVKKVIILLEGRVPENVLNPEVLKKLRLSA"
     gene            complement(3300..4592)
                     /gene="larA"
                     /locus_tag="VEL68_17870"
     CDS             complement(3300..4592)
                     /gene="larA"
                     /locus_tag="VEL68_17870"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HXZ37896.1"
                     /translation="MRENNSPHLEKIPYGSGELEVKIPRKNYVATLMPHFKPGLKDEA
                     GEVKKALENPIGTKRLKEIAKGRKSAVIVVNDITRPTATYKLLPPLIQELKNGGMKED
                     QIQLLVATGTHRDNTREELEAMLGKDVVQRFKVVNHHCQDDKIMVDLGKTSGGIPVVI
                     NRLFWEAEVKITTATIAPHQGAGFSGGRKSVLPGIASLSTLKQHHGFSMRSDKPAMGW
                     VEGNKFHENALEAAKMAKTDFILNTVQNHLKKITQVVAGDVEKAWLEGVKASREVFEV
                     RVPQPADIVITTPGGFPKDIDLYQSQKSMASAELVVKEGGTVILLAECKDGVGARGFY
                     EWMSAATCPQEVIDRFIREGYSIGASKAWLFSRCLKRSDLIVVSKHMDDKTLKEMFSQ
                     RADTFDQAIQMALAKHGENAKVILLKNGSDMIPRFGGK"
     gene            4874..>5608
                     /locus_tag="VEL68_17875"
     CDS             4874..>5608
                     /locus_tag="VEL68_17875"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXZ37897.1"
                     /translation="MPEYEIYALKYAGPFTSSGAFLMWRKDWEKQVQRNYYLWCVRGE
                     KDMVIVDAGVPPDLAKQKNLNGYVSPVVVLSRIGVKAEEVRHLLLTHIHWDHSSGVGL
                     FPNATVYVQEEEFNFAIKNPIANRPVFAATADPKSKAYIASLEGTKRLVLIRGDREIL
                     PGIECLLAPGHTVALQAVAVKTSKGTAIVGSDCAHTFQNYREDWPSCLIVDLVGWMKS
                     YDKLRKKASSPDLLFPGHDPLMSSNFP"
BASE COUNT         1124 a         1611 c         1478 g         1395 t
ORIGIN      
        1 tcctcccatg ctgctctcga tccgttcgga actcgggatg agccttttct gttcggtttg
       61 atcatgcagg gaggccaact agggcaggtc atcggtccgc cggtcttcgc ccttatggtt
      121 tccatgggtg aaggcgggaa atcagcttcc tggcttttgg gggtctcggc agccgccggg
      181 gttgggtttt ctttgggctt ggccgtcctg aaaaaaaggc ggcttcccat tgggccaagg
      241 aatcggccaa tcgtaatctg accagcttct gggaatcaat ccttcaccga gagagcttga
      301 atgacgatgg aaaccgcggc cttaaagctg agacgctctg tattcaggga caggtcatag
      361 tgtaccgggt cttcccaccc tgcatgataa tatcttttga tgaactcgcg actgccgttg
      421 tcaaagcgcg ccatctcctg cttggccgct tcctcaccca ctttgagctc ctcccggacg
      481 cgtttcaacc gcacttcgaa aggtgcgacc gtcagaatgt gaagggtcct gggaaagttt
      541 tttaaaatga actggctgcc ccgtccttga atcacaaggg agggggctcg agccagctcc
      601 ttaatcaccg actcaagggc ttgcaagtag cgtttatcat ccagggggat ttcccaggtg
      661 ggtagatagg taccctcaaa ccctacgccg aagggtaaac cccgatccaa tgcttcggca
      721 atccgcccaa cgagggtgct ggggggcatc tctttcgccg ctacgtcatg ctcttgacga
      781 tgaagtcgcg ctgccactcc ggcaatgatc tcccggtcga cgtagtcggc gtgaagaaat
      841 tcggcgattg ccttcccgat ttccggcgcc ccgcttccca agggtcctcg aatcgtcaca
      901 atcggcatcc gctacctcct tctttgagta tttatgctcc cctgcattca ttccatgatc
      961 cttcccgatt ctccggttga atcaagcaaa gagattggtg tacgtgatca ttcgacggcc
     1021 attccttccc ctttttctac atgccggcga tcttcgccgt gcgtcggacc acgtcgaaca
     1081 ggctgtcctc agcgattaca tgtcccgtca gcttcttttt gctcttggcg acggcctggc
     1141 gcccggcctc ggatttatcg aggtccacca gggcagcctg aattttcttg cttaaggcct
     1201 cgtccatgct gatccggaca gcgatggcgt cgtgggggat tggatcggtg ctggccaggg
     1261 tggtcagatt ctcggtggac atgcccatgg ccttcgcgac accgagggca ccgtcataaa
     1321 tcgccccggc atctgcccgc ccctccagga cagcggcgat cactttgtcg tggccgcctg
     1381 caaagatcgt ctccctgaaa tatcgctccg gggtgaaacc cttctcgatg agcatggcgc
     1441 gcgggtatac ataaccggag gccgatttgg ggtccacgaa ggcgaaacgt ttgccctgca
     1501 ggtcgtccag agtctttagc ccgctgtcct tgcgggccac gatgaggcca cgataggtgg
     1561 ccgccccatc gaggatggac tgggcgatga tccgaatctt tccgccccgc gccgcgtcca
     1621 cataggcaaa gggagagaag ctcccgatgt ctacgattcc cgcttccatt tgggtacgga
     1681 ggtctccata gctcttggcg acgtagagga caaccttctg cccaacggcg ttggacaaat
     1741 aagccatgag cggttcgaac tcggctttca tatccgcctc gctcaggact ggggtaaacc
     1801 cgaacttcag ctcttgggcg gcagagggca cggccgggag aagcccgata cccagggata
     1861 gaagaatcat ccccaggacg tatggatgac ggaggatttt tttcgctccg gataactcat
     1921 taccggcgtt ggataaattt ccaattcttt tcatttccgg ccctcctttt tttaaatatg
     1981 cttaccgata aaagaaatgc aaaaagaccc catctccctt caagaaatga ggtctttcat
     2041 cccgcttaac cacataactt tcgcctctgg ctgcaaggaa gggcatgggt ttgaccggaa
     2101 gggcccatag gcctgcacct ggaattggta agggaactat attaaatttt gcaaaacctg
     2161 tcaataaaac gagaaaaatg tattttgagg gataaagggt ggagaatggc aactcgtttt
     2221 gagtcacaac ttgtgggttg taggggcgat tcagccctcg acctgagctc gggccgaagg
     2281 tgaatcgccc ctacaacccc tatgcgctta gccttagctt tttgagcacc tccggattca
     2341 gcacgttctc tggaaccctt ccttcgagga ggatgataac cttcttcacc gtctccatgg
     2401 ccatagcatg ggtcgcttgg ttgctcaaac cggcgctatg gggggtggag accacgttgt
     2461 ccagcaggat gtggtcccgg aagaccggtg gttcggtctc aaaaacgtcg agtcccgccc
     2521 cggcgatcca gccttctttc aaagccttgt agagggcttt ctcatccacg atggcccccc
     2581 gggcagtgtt gatcagaaag gcggttttct tcatgagccg caagcgctct tcattgatca
     2641 tccttcgggt ggcagggtcc aaagggacgt ggagggtcac aaaatcggcc cgctgtagaa
     2701 gctcttcctt ggaaaaatag gggatattgt attgcttctg gaaatccggg tattccgcga
     2761 tgtcgttagc gattaccttg gcttcaaagc cctgaaggcg tttggccacc cgtttgccga
     2821 tgtttcccag gccgatgatc cccacggtct tccggaagag gtcctcgctt ccgctgccgc
     2881 gggggtataa tcccttgcgc atttcccggt ctttttgggt gatctgcttg gaaacggata
     2941 gcatcaatcc aatcgccatg tcggcgacgg actccgcatt ggcccccggg ttgttggtca
     3001 ccaccacccc tttctcggtt gccgctttca gatcgctccc ttcaaacccg gcgctgcgga
     3061 tagcgatcat cttcaggcgg tcggcgctca tgatgaactt gcgggtcgcg ggaaacatgg
     3121 cggtcacgat gatggcatcc gccccctgga tcgcctggca gagctcgtct tcttgggccg
     3181 gggatgcccg gtcgtagtcc ttttccacca cctccaatcc cgcctgtttc atgacttgaa
     3241 agggttcccg tgagaactcc gaaaatttct tggtcaatac cattacttta tacatttcat
     3301 cattttcctc caaaccgagg gatcatatcg gacccatttt tcaggagaat gaccttggca
     3361 ttctctccgt gcttggccag ggccatctgg atggcctggt caaaggtatc ggctctctgg
     3421 ctgaacattt ctttcagggt cttatcatcc atatgcttgc tcaccacgat caagtccgac
     3481 cttttcaggc agcgggaaaa caaccaggcc ttggaagccc caatgctgta cccttcccgg
     3541 atgaatcggt cgatcacctc ttgggggcag gtggccgcgg acatccattc atagaacccc
     3601 cgcgccccca ctccgtcctt gcactccgcc aggaggatga ccgttccccc ctccttgacc
     3661 accagttcgg cggaggccat ggacttctgg gattggtaca ggtcgatgtc tttggggaac
     3721 ccccccgggg tggtgatgac gatatcggcc ggttggggga ctcggacttc gaaaacctcc
     3781 cgactggcct tcaccccttc cagccaggct ttttccacgt ccccggccac gacctgggtg
     3841 atcttcttga ggtggttctg gaccgtgttg agaatgaagt ccgtcttggc catcttggcg
     3901 gcttccaggg cgttctcgtg gaacttattc ccctccaccc atcccatggc tggcttgtcc
     3961 gaacgcatgc tgaagccgtg gtgctgtttc agggtggaca ggctggcgat tccaggaaga
     4021 acgctctttc tccccccgga aaatcccgct ccctggtgag gagcaatggt cgccgtggtg
     4081 attttcactt ccgcttccca gaagagacgg ttgatcacca ccgggatccc accgctggtc
     4141 ttgcccaaat ctaccatgat cttgtcgtcc tgacagtgat ggttcaccac cttgaaccgc
     4201 tgcaccacgt cctttcccag catcgcttct agctcttccc gggtgttatc gcgatgggtg
     4261 ccggtggcca ccagtaactg gatttggtcc tctttcatcc ccccattctt caattcctgg
     4321 atgagaggag gcagaagctt gtaagtggcc gtgggacggg taatatcgtt gaccacgatg
     4381 acggcgcttt ttctaccctt ggctatttcc ttgagccttt tcgtcccgat cggattttcc
     4441 agggctttct ttacctcccc ggcttcgtct tttagtcccg gcttgaaatg gggcatcaag
     4501 gtggctacat aatttttccg gggaatcttg acctctagtt ctccactgcc ataggggatt
     4561 ttttctagat gcggcgaatt attttcacgc atgctcactc cttctagctt tagctccgaa
     4621 tcatttcaaa gatttcgggc acggagaaaa ggccaatcca cacgggcacc cgtttgacgg
     4681 cgatcctctt tccctttcat gaattttttc ttttttcatc ctccgagatg aatcatataa
     4741 ttggccggaa cagggtgtca agaagaatgc ccttttggga aagttgtagc ttctcccttt
     4801 ttctcatagt ataaaaagga aacggatttg aattccgtag ccgtctctga atcggacgga
     4861 atgaggagga ttcatgccgg aatatgaaat ctatgccctg aagtatgccg gaccgttcac
     4921 cagttccggt gctttcctaa tgtggaggaa agactgggaa aagcaggtcc agaggaacta
     4981 ttatctctgg tgcgtccggg gggaaaagga catggtgatc gtggatgccg gggttcctcc
     5041 cgatttggcc aaacagaaga atctaaacgg atacgtgagc ccggtggtag ttctttcccg
     5101 aatcggggtg aaggccgagg aggtccgtca tctcctcctt acccacattc actgggatca
     5161 ttccagcgga gtcgggcttt ttcccaacgc aactgtgtat gtccaggaag aagagttcaa
     5221 cttcgccatc aagaacccca tcgccaaccg tcccgttttc gccgccaccg ctgacccaaa
     5281 gtccaaggct tatatcgctt ccctcgaagg gacgaaacgc ctagtcctca taaggggaga
     5341 ccgggaaatc cttcccggga ttgaatgcct tctggctccc ggtcatacgg tcgctctcca
     5401 ggccgtggcc gtaaaaacct ccaagggtac ggcgatcgtc ggctccgact gtgcccatac
     5461 ttttcagaac taccgggaag actggcccag ttgcctcatt gtggacctgg tgggctggat
     5521 gaagtcctac gacaaactcc gcaaaaaggc gtcttcccct gacctccttt tccccggaca
     5581 cgacccgctg atgagctcca atttccca
//
LOCUS       DASPRR010000486         3859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_927755, whole genome shotgun sequence.
ACCESSION   DASPRR010000486 DASPRR010000000
VERSION     DASPRR010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927755"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VEL68_17880"
     CDS             complement(<1..1042)
                     /locus_tag="VEL68_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ37898.1"
                     /translation="MIIPEKMKAAVLVSPNVLAVREVETPKPGPADVLIRVASCAICS
                     SDLSLMAYPWPSQPPYGEFIPGHEYSGVIAAKGETVDEVHVGDRVSVEAHYGCGRCRN
                     CRLGDYTSCLNYGNPEKGHRANGFTTNGGYAQYVVNHVNTVYPIPDSIGFEEASLITN
                     LGCVLYGFETVGGYVVGDRVAVIGPGPLGLISVEVAKALCAGKVYLIGTRASRLQVGA
                     SLGADRLIDVNQEDPVQIIRKESGGVGADLVIESSGSAQGLQMAIAMTKRMGKILLLG
                     FPEEPVAADFASLARDNKSIYTVRGEGWGNVGRAVSLLASGRVDLKPLVTHSFSLEKI
                     EEGFKTFRERIGG"
     gene            complement(1164..2021)
                     /locus_tag="VEL68_17885"
     CDS             complement(1164..2021)
                     /locus_tag="VEL68_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HXZ37899.1"
                     /translation="MAKRKSFYLHDMNYTEVAAYLKSCDVVMVPMGSLERHGAHIPLG
                     CDALTTWEVATRAAKKAQVIYTPIIPYGYSPHHMHEMGNGTGTITLSGETYRRILYDI
                     GKSLIYHGFNKVVFVTHHGSNSKVVDEVLRRIRYESGAFTCWYKTPTEREYNVLKGII
                     EGPPEETPGWHSGEIETATCLAYDESLVDMSKAVQDRTHAPEWMGKAFSKKDGSGMVE
                     FMESEQAWVPMEHHEYSTTSTIGNPFRGTKEKGLKYFERSAEHLAAFLEEVKKFPIRA
                     TSREFNNRA"
     gene            complement(2435..3679)
                     /locus_tag="VEL68_17890"
     CDS             complement(2435..3679)
                     /locus_tag="VEL68_17890"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ37900.1"
                     /translation="MATQETKDMVDRCNKCGLCIPSCPVYQQVLTEAASPRGRVQLVK
                     NFLEGNVSLSKRAKEIILTCLLCESCVVNCPSGVRLDNLFTDFREELVKQYGLAWKKR
                     LLYTLLTNEKLLRSSILFAKLGRNWILETFAKGMRIGNIPAGKLPRVNPKPFRDQFEG
                     VIRPEGKAEGRVFYFTGCFTNYFAGDVGQAVVNVLKKLRLEIEMPKGQDCCGIAAILS
                     GEGDLPLKNVEKNISLLARAEVDAVLVDCATCGAAFKKEYIALLKRKGMDTTGAEALK
                     KKTLDVMEYVAARMEKLPLRKNGSRKKVRVTYHDPCHLVRAQGVSDAPRKILKALPQV
                     EYVEMEEANACCGGGGAFQFDFPAVSKGITEKKIKSIRQTGAAVIVTGCPGCRLTIGG
                     NMDEGDPISVLHPLQLLDEALR"
     gene            complement(3698..>3859)
                     /locus_tag="VEL68_17895"
     CDS             complement(3698..>3859)
                     /locus_tag="VEL68_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXZ37901.1"
                     /translation="LGGTLTGEHGIGIAKREILPLELGEAAMEVTRNIKKAFDPLNIL
                     NPDKVVRVL"
BASE COUNT          774 a         1139 c         1046 g          900 t
ORIGIN      
        1 ctcctccaat cctctccctg aaggttttga acccttcttc gattttctcc aaagagaagg
       61 aatgagtgac gagcggtttc aggtccactc ggccagaggc gagcaaagag accgcccttc
      121 ccacgttgcc ccatccttcg ccccgcaccg tgtagattga cttgttgtcc ctggccagcg
      181 aggcaaaatc cgcagccacc ggttcttccg ggaatcccag aagcaatatc ttgcccatcc
      241 gcttggtcat ggcaatggcc atttgcaatc cttgggcgct ccccgaggat tcaatgacca
      301 ggtcggcgcc gacgcccccg gactccttcc ggattatctg gacagggtcc tcctgattca
      361 cgtcgatgag gcgatcggct cccaaggacg cccccacctg aagtcgggaa gcccgggtgc
      421 cgatcaggta aaccttcccc gcacacaagg ccttggccac ttccacggag atcaggccca
      481 agggtcctgg gccgattacc gccacacgat cgccgacgac gtagccaccg acagtttcaa
      541 acccgtacag gacgcatcct agattggtga tcagggacgc ttcttcgaaa ccgatggaat
      601 ccggaatcgg gtagaccgta ttcacgtggt tgaccacgta ttgggcgtac ccgccgttcg
      661 tggtgaatcc gttcgcgcgg tgtccttttt ctgggttccc gtagttgagg caggaggtgt
      721 aatcccccag gcggcaattg cggcacctcc cacagccata gtgagcttcc actgagaccc
      781 ggtcaccgac gtgaacctca tccacggtct cccctttcgc cgcaatgacc ccggagtatt
      841 catgaccggg aatgaattct ccgtaggggg gctgactggg ccaggggtag gccatcaggc
      901 tcaaatcgct gctgcagatg gcgcaggaag cgacccggat gaggacgtcg gccggtccgg
      961 gtttgggagt ttccacttcc cggaccgcca aaacattggg agagaccaaa acggcggctt
     1021 tcattttctc cggaatgatc attctatttc cttcgtattt catgtagggt gcgttccccg
     1081 aacgcaccgc catgtcaggc ccgatcgggg ttcaggccct acaaatctca aacttctgac
     1141 cccgcacccc gaacctgttc tactcaggcc cggttattga actcccggct ggttgcccgg
     1201 atggggaatt ttttcacttc ctccaggaag gcggccagat gctcggcgct gcgttcgaag
     1261 tatttcaatc ccttttcctt cgtgccccgg aaggggtttc cgatggtcga agtcgtggag
     1321 tactcgtggt gctccatggg gacccaggcc tgctcggact ccatgaattc caccatcccg
     1381 gagccgtcct ttttggagaa ggccttgccc atccattccg gggcatgggt gcggtcttga
     1441 accgctttgg acatgtccac cagggattca tcgtaagcca ggcaggtcgc ggtttcgatt
     1501 tccccggaat gccagccggg agtctcctcc ggagggcctt cgatgatccc tttcaggacg
     1561 ttatactccc tttccgtcgg ggttttgtac caacaggtaa aggctccgct ctcgtaacga
     1621 atgcggcgta gcacctcgtc caccaccttg gagttggacc catggtgggt gacgaaaacg
     1681 accttgttga acccgtggta gatcaaggat ttgccgatgt cgtacaggat ccgacggtag
     1741 gtctcccccg agagggtgat cgtcccggtt ccgtttccca tctcatgcat gtggtggggc
     1801 gagtagccgt agggaatgat gggggtatag atcacctggg ccttcttagc agcgcgggtg
     1861 gccacttccc aggtggtcaa agcatcgcag cccagcggaa tgtgcgcccc gtgtctctcc
     1921 aggcttccca tgggaaccat gacgacgtca caggatttta ggtatgcagc aacctccgta
     1981 tagttcatgt catgcagata aaacgatttt ctcttggcca tattttcctc cggtgatttt
     2041 gataaaattg ggttttgttc gaaaagtaca tgattcgtct tgcgagaggt caaaatgaaa
     2101 aaacttgaaa tttgtcaaaa aatcttgttt ggtcaaccgt ttctgaaatc ccccccaacc
     2161 ccccctttga taaagggggg caggggggat ttctggcagg gccgtggttc ttgggaaaat
     2221 cctggatgct ggttgcggtt ctgccaatct acgaattact cctgaaaagt gatccatcaa
     2281 gtatttttcg aacaaagcct aaaattggtt tctctccggc gtccccggtg cgcttttagg
     2341 ggcaagaggc atcgcgcccc tacggcctcg attaaggata tatctttctt accaacatat
     2401 taatattggt tgggcgctaa gcgcctaagg gcctttatcg gagcgcctca tcgagcaact
     2461 gcaggggatg aaggacggag atcgggtccc cctcatccat attcccgccg atggtcagac
     2521 ggcatcccgg acatcccgtg acgataactg cggcacctgt ctggcggatg cttttaatct
     2581 tcttctcggt gattcccttg gagaccgcgg gaaaatcgaa ctgaaaagcc ccgcccccgc
     2641 cgcagcaggc gttcgcctcc tccatttcca cgtattcaac ctgggggagc gccttgagga
     2701 tttttctcgg agcatcgctc accccctgag cgcgaaccag gtggcaaggg tcatggtagg
     2761 tgacgcgaac ctttttcctg gacccgttct tgcgcagcgg cagtttctcc attctcgcgg
     2821 caacatattc catgacgtcc agggtctttt tcttcagggc ctccgcccct gtggtgtcca
     2881 tccccttgcg cttcagcagg gcgatatact cttttttgaa ggcggccccg caggtggcgc
     2941 agtccaccag gacggcatcc acttccgccc gggcaagcag ggagatgttc ttctccacat
     3001 ttttcagggg caagtctccc tcgccggaga ggatggcggc gatgccgcag caatcctgcc
     3061 ccttgggcat ttcgatctcc aggcgcagct tcttcaaaac gttgaccacc gcctgaccca
     3121 cgtcccccgc gaagtaattc gtgaaacagc cggtaaaata gaaaacccgg ccctctgctt
     3181 tcccttcggg gcggatcact ccctcaaact gatcccggaa aggcttggga ttgacccgcg
     3241 gcagctttcc cgccgggatg tttccgatcc gcatcccttt ggcgaaggtt tccaggatcc
     3301 agttgcggcc cagcttggcg aagagaatgg aggagcgaag aagtttttcg ttggtcagca
     3361 gagtgtagag cagacgtttc ttccaggcca gtccgtattg cttgaccaat tcctctcgaa
     3421 aatcggtgaa caaattatcg aggcggacgc cgctgggaca attgacgaca caggattcgc
     3481 agaggaggca ggtcaggatg atctccttgg cccgcttgga gagggagacg tttccctcca
     3541 gaaaattttt caccagctga accctgcccc gggggcttgc cgcctcggtc aaaacctgct
     3601 gataaacggg gcacgagggg atgcagagcc cgcatttgtt acagcgatcg accatatcct
     3661 tggtttcttg cgtagccatc cattcgctcc aagccgccta caggacccgg acgaccttat
     3721 ccgggtttag gatattcagg ggatcaaagg cctttttgat attccgggta acctccatcg
     3781 ccgcttctcc caactccagg ggaaggatct cccgtttggc gattccgatt ccatgttctc
     3841 cggtcagggt gccccccag
//
LOCUS       DASPRR010000487         6206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1410447, whole genome shotgun sequence.
ACCESSION   DASPRR010000487 DASPRR010000000
VERSION     DASPRR010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410447"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VEL68_17900"
     CDS             <1..337
                     /locus_tag="VEL68_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37902.1"
                     /translation="QSYQLGPDNTPNTYSAGSDLLSWEFEYKMDGPEITFTFIPETYS
                     LEYLEGPNKGLKLLPPNNAVFDKPWTGRISPDGRNLFVFYGGPAKLEIPEFGIELICN
                     AVHQGFKTH"
     gene            639..1937
                     /locus_tag="VEL68_17905"
     CDS             639..1937
                     /locus_tag="VEL68_17905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ37903.1"
                     /translation="MRVALIAPAPASYIWRKKRAAFTLPPMALPLLAALSPRGVEVRL
                     IDEAVEDVGLNLEADLVGLSVMTATAPRAYRLGDHFRSRGIQVVMGGVHPSSLPEEAL
                     QHSDAVVIGEGEFLWLRILKDADQGSLKRTYQNPSTFPLDRLPAPRWDLLPAQRYFVP
                     RTVQVSRGCPMSCSFCSVSSFFGRSYRQRSTSQVIEEIRAHKKRLLIFADDNIAGNPE
                     AAKELFAAMIPLKKKWVSQCSLSVADDPELLDLAARSGCIGLLIGFESISPEVLKSIG
                     KRVNLHRQYQEAIRKIHSRGIHIQGSFIFGFDEDTPESIPATVRFVKENRLTGVNYCR
                     LTPFPGTKLYADLEREGRIIDRDWSNYDRQNVVFQPRNFTPQDLQEKIFWAYSQTYNL
                     RSLWQRRPFSFQHFSLYLLLNFGYMKGLKKMEREVMRRTA"
     gene            complement(2341..4071)
                     /locus_tag="VEL68_17910"
     CDS             complement(2341..4071)
                     /locus_tag="VEL68_17910"
                     /inference="COORDINATES: protein motif:HMM:NF036774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HXZ37904.1"
                     /translation="MQAKRRIGTVALILLLGLFFPGLFPAESRAASKTRVAVIDFEQK
                     AFQELQGKQIGEIVAEWLITSMANTGRFEVVERAQLQKILREQQLGVSGMINQETAAK
                     MGELLGVKVIVTGSVIQIGNSYDVNARLISVQDGTIIKAERIRGVGLDGVERMMDSLA
                     DSFKKEFPLEGYVVMVSGKRAMIDLGRINGVEPGMRFFALRKGTPVRHPVTGKMLAGE
                     DIKIGEMIVQTAEREASWADIVQQEPGISIAAGNLAKQALGETMAVTTTPLAPLPPPA
                     PSVTVPPPSPPTPSPAAPVVPPPKMQPTIPGRTMGNRILVEWAGMHDLLLGDRDGYVW
                     LFLNIGGKESPRYDAGTKVKAGGKEIKVRGPSYPYLVDWNEDGKVDLLVGNGGGYLHL
                     FLNQGDGNFASGVMVKAGGKDLDVGSRASPCVVDWNEDGKKDLVMGNGSGEIEVFLNE
                     GTNQQPAFGKGIKLNNGKLDVGSDSSPDVVDWNGDGKKDLVVGNDNGEIFIFINQGTN
                     REPQFNNDGEKLPLKLGPSAIPQVLSRGRGLNDLVVADRNGEVTYCSNAGSAAAPSFP
                     EKRIVKAGKR"
     gene            complement(4095..4790)
                     /locus_tag="VEL68_17915"
     CDS             complement(4095..4790)
                     /locus_tag="VEL68_17915"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ37905.1"
                     /translation="MVEEKTIWAIIPAYNAESSVGEIIERTKKFIPRVIVVNDGSTDR
                     TEDVARNRGTEVVSLPSNRGKGFALRQGFGLALSNGCKAILTLGADGQHDPADIPNFL
                     AAHEQDTQAILIGSRMAQADRFPTQRYYSNRTAAFFISKALGQYLEDTQCGFRLYPAD
                     TLRSIPLTTSHFQTETEVLLRAVQRGIRLGSVPVKNIYHSNGNAPQSNFRPVIDTFYI
                     CMVVLQSYLKLLN"
     gene            4997..>6206
                     /locus_tag="VEL68_17920"
     CDS             4997..>6206
                     /locus_tag="VEL68_17920"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXZ37906.1"
                     /translation="MILAVLSGFLITRLHFQSDVLNLLPGNAPRTQDLVKFLKEFGSG
                     DSLYIVLDRKSGGEVESLIPFVEALADRLMATGEFTEIIGRMEPEVKEKMARLFLRKA
                     ILYLPKEDLKTIEWKLSDRGIEEQIRLLKTKVSSLFASDLATYDPLELFPLFQKNIPL
                     PPHMTDFDSKGYLISTDKKIILLIAKPRGSAPDVDYDEMLVNKVRGAEIAARQAFSQT
                     PGNFSSGLESDLRMGLTGGFIHALEDSRMIKSDLVWNFSLSLVGVLSLIFLAFRIGAL
                     FLYALFPLLVSPLLTLGLLSPFLGRLNESTGAFSAIILGLSIDFIILLYSRYQEEKSA
                     GTEVSGALNRSLITVGPGIFTGAITTTAAYYALLVSDFRGVRDLGLLTGTGILVSLAC
                     AFLLFPALVAW"
BASE COUNT         1285 a         1814 c         1589 g         1518 t
ORIGIN      
        1 gcagtcttat cagttagggc cggacaatac gccgaacact tacagcgcag ggagcgacct
       61 tctatcgtgg gagtttgaat ataaaatgga tggacctgag attaccttca cgtttatacc
      121 tgagacctac tctttggagt atcttgaggg cccgaataaa ggccttaaac tgctcccgcc
      181 gaacaatgct gtatttgaca aaccatggac gggacgtata tcaccggatg gcagaaacct
      241 attcgtattc tatggagggc cagctaaatt agaaattcca gaatttggta ttgagttaat
      301 ttgtaacgca gttcaccagg gattcaaaac gcattagtaa tcaggcgaga agtctagtat
      361 cgcagaggtg aaggattcaa acactcttga agggcagtcg gtttttcggg cgcattggtt
      421 gggtgccact ccccaagcta ccctgaacta agctaattag tcatcgaaaa caaggaggca
      481 gggtcaggaa tgatcctgcc ttttttgtgc ctacatatag tagattcggg cgtctggggt
      541 aaaatgaagg gggattcatc cttgacagtt tccacccttc ggccccataa ttctttccac
      601 tggaagaagc gaattcttat catcgcgggg atgaagatat gcgtgtcgcg ctgatcgctc
      661 cggccccggc ttcttatatt tggaggaaga aacgggcggc tttcacttta ccgcccatgg
      721 ccctgcccct tctggctgcg ctttccccgc ggggggtgga ggtccgactc attgacgaag
      781 cggtggagga tgtgggcctt aacctagagg ccgaccttgt ggggctgtcc gtcatgaccg
      841 ccacggcccc aagggcttac cggttgggcg atcactttcg gtcacgggga atccaggtcg
      901 tcatgggcgg ggtgcatcca tccagccttc cggaagaggc cctgcagcac agcgacgcag
      961 tggttatcgg ggaaggagaa tttctctggc tccggatttt aaaggatgcg gaccaaggaa
     1021 gtttgaagag gacctaccaa aacccctcca cttttccctt agaccggctg cccgctccca
     1081 ggtgggacct tcttcctgcc caaaggtatt ttgttccccg gacggttcaa gtgagccgcg
     1141 gttgccccat gtcctgttct ttttgttccg tgagctcctt tttcggccgc tcctaccggc
     1201 agcgctccac ttctcaggtc atcgaagaaa tccgggccca caagaaaagg cttctgatct
     1261 ttgcggacga taatatcgcc ggaaatccgg aagccgccaa ggaactcttt gccgccatga
     1321 tccccctgaa gaagaaatgg gtctcccagt gttccctgtc cgtcgccgac gatccggagc
     1381 tcctggacct cgcggcccga agcggatgca tcgggctgct catcggtttc gaatcgatct
     1441 cccccgaggt tctaaaatct atcggcaaac gggtcaatct ccaccggcag taccaggagg
     1501 ccatccgaaa gattcattcc cggggcattc acatccaggg ttctttcatc ttcggattcg
     1561 atgaggatac cccggagagc atcccggcca cggtccgatt cgtaaaggaa aaccggctca
     1621 ccggtgtgaa ttactgccgg ctgaccccct tcccggggac caagctctac gccgatctgg
     1681 aacgggaagg aaggatcatc gaccgggact ggtccaatta cgaccgacag aacgtcgtct
     1741 tccagccgcg caactttacc ccccaagacc tgcaagagaa aatcttctgg gcctacagtc
     1801 agacctacaa cctgcgttcc ctctggcagc gacgcccttt ctcgtttcaa cacttcagcc
     1861 tatatctttt actgaacttc ggctacatga aggggctgaa aaagatggaa agagaagtaa
     1921 tgagaagaac cgcctgaatg tggattgtga aatgcggaat cctggtcgaa atttttttta
     1981 catcttcttc acgtccgcct gcccgatctt gaggtagagt ttttccttct ttggcttcag
     2041 gttgatccct tggcgagaag ctccgccgcc ctcggtgctt cccccttagc agccagtaac
     2101 gcgacggcgg agatgaatcc tttcttcata aggccttctc ccaagaaagt ttcaagtctc
     2161 taatttcggg tttcatttcc tatctctcga tgtcctctgt ggccctaagg tccttaccgg
     2221 atttttgcct gcaggtcctt taccgccagt tgcacggttt catccgccac ctggctcaaa
     2281 ggacgtatga cgctccccat ttgccggtca tctgctgttt ccagagaatc atcattcccc
     2341 ctagcgcttt ccggccttca cgattctctt ttccgggaag ctcggcgccg cagcgctccc
     2401 cgcattggag caataggtca cttccccgtt tcggtcggcc accaccaagt cgttcagtcc
     2461 cctccccctg ctcagcacct ggggaatggc actcggaccc agcttgaggg gtagtttttc
     2521 cccgtcgttg ttaaactggg gttctcgatt ggtcccctgg ttgatgaaga tgaagatctc
     2581 tccgttgtca tttccaacca ctaaatcctt cttgccgtcc ccgttccaat ccaccacgtc
     2641 gggggaggaa tcggacccta catccagctt tccgttgttg agtttgatgc ccttcccgaa
     2701 ggccggctgt tgattggtcc cttcattgag gaagacctcg atctccccgc tcccgttgcc
     2761 catcaccagg tcctttttcc cgtcctcgtt ccaatccacc acgcagggag aagcacggct
     2821 tcccacgtcc aagtcttttc cccctgcttt caccattact ccggaggcaa agttcccgtc
     2881 tccctggttc aggaaaagat ggaggtatcc acccccgttc cccacgagga ggtccacctt
     2941 tccgtcctca ttccagtcca cgagataagg gtaggacggc ccccgcacct tgatctcctt
     3001 gcctcctgct ttcaccttgg tgccggcatc atagcggggg gattccttac cgccaatgtt
     3061 caggaagagc cagacgtatc cgtcacggtc acccaggaga aggtcatgca tgccagccca
     3121 ttccaccagg attctgtttc ccatggttct tcccgggatc gttggctgca tcttaggggg
     3181 tggaaccacc ggcgcagccg gggacggtgt aggaggggac ggaggcggta cggtcaccga
     3241 aggagcgggg ggcgggagag gcgccagcgg cgtggtcgtt accgccattg tttctcccaa
     3301 agcctgtttg gccaggttcc cggcggcaat ggaaatcccc ggttcctgct ggacgatatc
     3361 ggcccaggac gcttcccttt ctgccgtctg aacgatcatc tctccgatct taatgtcctc
     3421 tccggccagc atcttgccgg tgaccggatg gcgcaccggg gtccctttgc gcagggcgaa
     3481 aaacctcatc cccggttcaa ctccgttgat ccttcccagg tcgatcatgg cccgttttcc
     3541 gctgaccatg accacatagc cttccagggg gaattccttc ttgaaggaat cggccagaga
     3601 gtccatcatc cgctcgaccc cgtccagacc cacgccccgg attctttccg ccttgataat
     3661 cgtgccatcc tgcacgctga ttaggcgggc attgacatcg taggagttcc cgatctgaat
     3721 gaccgacccc gtaacaatga ccttgacccc cagcagttcc cccattttgg ccgcggtctc
     3781 ctggttgatc atcccgctga cccccaactg ctgctccctc aatatctttt gcagctgggc
     3841 gcgctccacc acctcgaacc tccccgtgtt ggccatggag gtgatcaacc attcggcgac
     3901 gatctccccg atctgtttcc cctggagttc ctggaaggct ttttgctcga agtcgatcac
     3961 cgccacgcgg gttttcgaag cagcccggga ttctgcgggg aaaagcccgg ggaagaacag
     4021 acccagcaga aggataaggg ccacggttcc aattcttctc ttcgcttgca tagaatccct
     4081 cctggatgag gaagctaatt cagtagcttc aggtagctct gaaggacgac catacaaatg
     4141 tagaaggtgt cgatcaccgg gcggaagttg ctctggggag cgtttccgtt cgaatggtag
     4201 atattcttta cgggaacgct gcccaggcga attccccgct gaacggcgcg cagcaggact
     4261 tcggtttcgg tctggaaatg agaggtggtc aatggaatcg accggagggt atccgcggga
     4321 tagagacgaa agccgcactg ggtatcttcc aggtattggc caagggcttt ggagatgaag
     4381 aaggccgcgg tccgattgga atagtaacgt tgggtgggaa agcggtcagc ctgagccatg
     4441 cgcgatccaa tcagaatggc ctgagtgtcc tgttcgtggg cggctaagaa attggggata
     4501 tccgccggat catgttgtcc atcagcaccc agcgtcaaga tggccttgca accgttgctc
     4561 aaggccagtc caaatccctg tcgaagagca aaaccctttc ctcggttgga agggagggag
     4621 acgacttctg tgccccggtt gcgggctacg tcctccgttc gatctgtgga cccgtcgttc
     4681 accacgatca cccggggtat gaatttcttt gttctttcaa taatttcccc taccgaactc
     4741 tcggcgttgt aggcaggaat gatagcccag atagtctttt cctccaccat ctctcccttg
     4801 catcttgaca gcccaaattt caggctccta ctggtcaaag cagcggggcc cgcttgaaga
     4861 ctctaagaat ttaatatttt taccaaatat gccttataat tagcaatagc aaaatatgaa
     4921 caccatccga aatcaaatat tcgccaggat cgctcaattt tccttttttc gctggcgacc
     4981 cctgatttgg ggcgctttga ttctagcagt tctttccggg ttcctgatta cccgtcttca
     5041 ttttcaaagc gatgtgctca acctccttcc ggggaatgct ccccggacgc aggatttggt
     5101 aaagtttctt aaagaattcg ggtccggaga ttctctgtat atcgttctgg accgcaaaag
     5161 cggaggggag gtcgaatcgc tcatcccctt tgtcgaagcc ctagcggacc ggctcatggc
     5221 tacgggggaa ttcaccgaga ttatcggccg gatggaaccg gaggtgaagg agaaaatggc
     5281 ccgcctgttc ctccgcaagg ccatcctcta tttacccaaa gaagacttga agacgataga
     5341 atggaaactt tcggaccgcg gaattgagga acaaattcgg ctcctgaaga ccaaggtttc
     5401 ttccctgttt gcttccgatc tggctactta tgatccgctc gagcttttcc ctctatttca
     5461 aaaaaacatt ccccttcctc ctcatatgac ggacttcgac tctaaagggt acctaatttc
     5521 taccgacaaa aagataatcc tcctgatcgc caaacccaga gggtcggctc cggatgtgga
     5581 ttacgacgag atgctggtga ataaagtccg tggcgcggaa atcgccgccc gccaggcctt
     5641 cagccagacc ccaggcaact tctcctccgg gctagagagt gatctccgaa tgggcctcac
     5701 cgggggtttc attcacgccc tggaagacag ccgcatgatc aagagcgacc tggtttggaa
     5761 cttttccctt tccctggtag gggttctttc ccttatcttt ctggccttca gaatcggggc
     5821 gttattcctt tacgccctct ttcccctcct ggtcagccct ctgcttaccc tcggccttct
     5881 ttcccccttc ctggggcggc tgaatgaatc caccggagcc ttttcagcca tcatcctggg
     5941 cctatccatt gacttcatta ttctccttta cagccgctac caggaagaga aaagcgcggg
     6001 cacggaggtt tccggcgccc tgaaccgaag tctaatcacc gtgggaccgg ggattttcac
     6061 tggggccatc accaccaccg ctgcttacta tgcccttctc gtatcggact ttcggggtgt
     6121 gagagacctg ggccttctca ccgggaccgg cattctggtc agcctagcct gcgcttttct
     6181 tctctttccc gccctggtgg cctgga
//
LOCUS       DASPRR010000488         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1568181, whole genome shotgun sequence.
ACCESSION   DASPRR010000488 DASPRR010000000
VERSION     DASPRR010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568181"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VEL68_17925"
     CDS             <1..401
                     /locus_tag="VEL68_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate synthase subunit beta"
                     /protein_id="HXZ37907.1"
                     /translation="IPYVGVVNGADPQDVFQTFQKIKNIQGPRFVHLFSPCPPGWRFD
                     PSEAVEIARLGVKSGMWILWEQENGIFSLKKGSRRYLDKANRIPVDKYLKSQGRFTLA
                     SKEKIRQLQDWVDQNWEEVRRRLTCEGEGR"
     gene            complement(499..1854)
                     /locus_tag="VEL68_17930"
     CDS             complement(499..1854)
                     /locus_tag="VEL68_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXZ37908.1"
                     /translation="MLQKLASASTRWSTRWVPDAWVIAVILTIVAYFLGIFFTKSNPY
                     QLVQFWGRGFWVLLSFGMQMCLIIMTGYILATTPVFSRILNALAGWPKGPKGSIALMA
                     LVSLVLAWINWGLSIVGSAVFVRFMARKQKGVDYRLLVATAYLGLGTMWHSGLSASAP
                     LLVATPKHFLEKEIGIIPITQTIFHPFNLLLALAVLIVLTIIGPLMHPKKGEEVEAKP
                     ELVKESGFEAPPKPKPEEMSPALSIENSPVINLIVGISGVVWLCWYFGQRGFAGINLD
                     VVNFAFLMVGVLLHWKPTSFLKAAQEGGSFIWGVVVQFPFYAGIFGIIQFSGLAEVMA
                     GWFTAIATKETYPFIVVWYSGILNYIVPSGGSKWAIEAPYIMQAAKNLGVSGPMTVIA
                     YAWGDMVTDVIQPFWAIPLLGVAKLSFRDIMGYCMVYFLIYMVLISIGFLLLPVFFAV
                     A"
     gene            complement(1976..>2324)
                     /locus_tag="VEL68_17935"
     CDS             complement(1976..>2324)
                     /locus_tag="VEL68_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37909.1"
                     /translation="LKNKIRKTLREKASPRHVPAIVMEMPEAPYTLNMKKVESAVTNI
                     IHGRPVLNRDALMNPDVLDYFEKILPELKKEEGRDCYFQGNPLRIFQGNHEEISKKPA
                     FIQRSKGGEGFIE"
BASE COUNT          554 a          657 c          643 g          470 t
ORIGIN      
        1 aaatccccta cgtcggggtc gtgaacgggg ccgatcccca ggacgtcttc caaaccttcc
       61 agaagatcaa gaatatccag ggacccaggt ttgtccacct cttctccccc tgccccccgg
      121 ggtggcgatt cgatccctca gaggccgtcg agatagccag gctgggcgtg aaatcaggca
      181 tgtggatcct gtgggagcaa gagaacggaa tcttcagcct gaagaagggt tcccggagat
      241 atctggataa agcgaaccgg atccccgtgg ataaatacct taagagccag ggaagattta
      301 ccctcgcctc caaagaaaag atccgccagc tccaggattg ggtcgatcaa aactgggaag
      361 aggtgcgccg gcgattgact tgcgagggag agggcagata gaaatgcaat accggtaggg
      421 tcccaacgtt gtaatggcgc ccgacccccg gggctcgagg gccgggcggc ggtatgctcc
      481 tactctttct tcaggccttc aagcaacagc gaagaagact ggaaggagga ggaagccaat
      541 ggagatgagc accatgtata tgaggaaata aaccatgcag taccccatga tatcgcggaa
      601 actcagcttg gccacgccca aaagaggaat cgcccagaag ggctggatta cgtctgtgac
      661 catatccccc caggcatagg cgatgacggt catgggcccg ctcacgccga ggttcttggc
      721 ggcctgcatg atgtagggag cctcgatcgc ccacttcgac ccacccgaag gcacaatgta
      781 attgaggatc ccggagtacc agaccacgat gaaaggatag gtctccttgg tggcgatggc
      841 ggtgaaccat ccggccatca cctcggccaa cccggagaat tggatgatcc cgaaaattcc
      901 cgcatagaag gggaattgga ccaccacacc ccagatgaac gacccgccct cctgggcggc
      961 cttcaggaag gaggtgggtt tccagtgcag gagaaccccg accatcagga aggcaaagtt
     1021 caccacgtcg agattgatcc ccgcaaaacc tctctgcccg aagtaccagc aaagccagac
     1081 caccccggag atgcccacga tcaggttaat gacgggggaa ttttcaatgg acaatgccgg
     1141 gctcatctct tcgggcttgg gcttcggcgg cgcttcaaag ccagactctt taaccagctc
     1201 cggtttcgcc tcgacctcct cccctttttt gggatgcatc aggggcccga tgatggtcag
     1261 gacgatcaag acggccaggg ccagcaggag gttaaaggga tggaagatgg tttgggtgat
     1321 ggggatgatc ccgatctcct tctccaaaaa atgcttgggc gtggccacca gaagaggagc
     1381 cgaggcggag agtcccgagt gccacatcgt gcccaggccc agataggccg tggccaccag
     1441 cagccggtag tcaactccct tctgcttccg cgccatgaac cgcacgaaaa ccgccgagcc
     1501 tacgatgctc agaccccagt taatccaggc caggaccagg gaaacaaggg ccatcagggc
     1561 gatggaacct ttggggccct tcggccagcc tgccagggcg ttcaggatcc ggctgaaaac
     1621 cggagtcgtg gccaggatat agcccgtcat gatgatcagg cacatctgca tgccgaaaga
     1681 gagcaacacc cagaagcctc ttccccagaa ctgaaccagc tgataggggt tcgactttgt
     1741 aaagaagatc cccaggaagt aggcgactat ggtgagaata accgcaatca cccacgcatc
     1801 gggtacccag cgggtgctcc aacgggtgct ggccgacgca agtttctgta gcatgttagc
     1861 ctcctttgca gaacggtgga ttatttctca gatctgaccg ctatgttctc cttctgttaa
     1921 agcatctggc ctttggatat tcaccccact cttcaaagga atctttcttc ccccttcatt
     1981 ctataaaacc ttcacctcct ttcgatcttt gaatgaaggc aggcttcttc gagatttctt
     2041 catggttccc ctggaaaatc cgcagggggt tgccttgaaa atagcagtcc ctcccttctt
     2101 cttttttcaa ctccggtaga attttttcga agtaatccag cacatcgggg ttcatcaggg
     2161 catcccggtt cagaaccggc cgaccatgaa tgatgttggt tacggcactc tcaaccttct
     2221 tcatattcaa cgtgtaggga gcttcgggca tttccatgac gatggcgggg acatgccggg
     2281 gggaggcctt ctcccgcagg gtcttgcgga tcttattctt cagt
//
LOCUS       DASPRR010000489        14815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1568851, whole genome shotgun sequence.
ACCESSION   DASPRR010000489 DASPRR010000000
VERSION     DASPRR010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568851"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VEL68_17940"
     CDS             <1..219
                     /locus_tag="VEL68_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ37910.1"
                     /translation="RDPQVVQRKMVTEFVDPRKGKMKLLSSPIKMSETPPDIRSAPAV
                     FGEHTEEILKELGFREEEIRHMKKEGVV"
     gene            485..1351
                     /locus_tag="VEL68_17945"
     CDS             485..1351
                     /locus_tag="VEL68_17945"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37911.1"
                     /translation="MNIREQRIRNLFNRETGKAVIIPMDHGIQGVREGLEDPLGSFQK
                     FAGLCPEAVLMNFGIVKLVQNIISGATDPPGIIMTTDFNHRWHSWKAPIEEKGLLGHC
                     ISADVEQAARYNVDAVKVLFDLGLNPKTQLSVFKNISELVRQCDRYDMPIMIEPLADG
                     LFIPSEKKNHPKIIADGCRIALELGADIIKAAYPGEGPESKEQFAAICKNAPVPVVIL
                     GGAKKDSIREIFQIARDGIDAGAKGVIFGRNVWQRPTHAMERVVKGLQDIVHRNAGVQ
                     ESLSKYNLPGEK"
     gene            1403..2467
                     /locus_tag="VEL68_17950"
     CDS             1403..2467
                     /locus_tag="VEL68_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXZ37912.1"
                     /translation="MPEKTRFGVIVSDRLAEVHEHKIPSLLSTEVLIENKSCNICTTD
                     YQQWMGLRSQQPTPMAFGHENCGIVIEVGSEVENARVGDHAVINNYRPCMECGDCRKG
                     RNFVYCKFRKSARTGPADAYGYFGNYGCSRYQIAKSKHIFKVNKDVPFELAGFAEPLS
                     TVVYGIKRLRVKAGEKLLVIGAGTMGNLNAQVARYFGADVTLSDVSEKKLKTAQRLRF
                     ERTLNARDTDYLKKARALTDGAGFDSIIIAVGLTEAYNHAIEMVADGGKLLIFAAGYP
                     KPGWNIDPNTVHYKLWEIIGTFGCEAADFQEAVGLLSDKRIDLAPLIEDRIPLKEIQH
                     AFEKASAPDSYRVAVLLGDS"
     gene            2684..4276
                     /locus_tag="VEL68_17955"
     CDS             2684..4276
                     /locus_tag="VEL68_17955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXZ37913.1"
                     /translation="MRYVLGVDIGTQGIKGVLLDEALNVAAKAYREHDYIQPRPDWFE
                     HDAEKTWWGGFKEIVHQLLKKAPISSSEIIGAGYSAIAPCMLPVDEDGRPLRNAILYG
                     VDTRSKKEIIEITQMLGEEKILETAKRPLGTDLIPPKMLWYKRNEPDKYSKTKRIFTA
                     SNYITYKLTGRFMLDYTQAAGFGPIYDFGKKQWNGEMCELLGFSSDLLPELKHCYDIA
                     GTVTPRASAETGLMQGTPVVVGSGDYATELISAGGFGPGEVTLIYGTTAIISMMTDRA
                     PSVKGLSVTPHPILENCFFVNGGTATMGALTKWFRDNFGDLEKIMQDRTGINAYTLLS
                     CQAEKIPVGAEGLVILPYFSGERAPIHDPLAKGVILGLTTYHKRAHLYRALLEGTAYS
                     LRHNFEVFDKCGFDVSRVIACGGGANSNLWVQIVGDVIGYDQSIPNIPIGSDIGSAYL
                     AAKGTGLIADLASFITNRRQKDARTVRADPQNHARYTDYYRIYRRLYESVKSDMHALA
                     ELAENHMGKMAEGSFRLGEADR"
     gene            4585..6003
                     /locus_tag="VEL68_17960"
     CDS             4585..6003
                     /locus_tag="VEL68_17960"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXZ37914.1"
                     /translation="MKHRMRTRRWMIAAAFLILGGWICWAAVEALAAEPDLTYFRQAK
                     INWRQFEGQKLTIGLNKHPYTESLLPLIPEFEALTGIKVEYLILPENEYFAKLVADLS
                     NARGEFTVIMTGPMRNWTYAAPGWILPLEPFLNDPKLTDKAWYKPEDFFPGLIAANRW
                     NGKIGGGTGEGALWSIPIMCESYILAYRKDIFDKYKIKVPTTYEELAEAARLVKKNAG
                     IDGVMARGTPNTPTISTGYISGAKSYTDGKWAEADQNMIARLSDPRHVNFSKIWIDMV
                     RESGPANWANMTWYDGMETFAAGQAGMYPDCDFFAQTYEDPKKSKVAGKVGYALLPPG
                     PGGRTYSGLWTWALGMSKATKNKEAAWYFIQWATSQRTLLNATVEFRNYNPSRASVMN
                     DPRTQKVLGSWGNGAYVKTVQENLKTARVAWVPNPERGRLGDIWARALHEIYFKRLSP
                     EEAMKRADEEINKIFKDIGLQK"
     gene            6083..6985
                     /locus_tag="VEL68_17965"
     CDS             6083..6985
                     /locus_tag="VEL68_17965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ37915.1"
                     /translation="MSRPHAPARQRDAILPYLLLSPGLLLLLGMLYPFFLGVYYSLTN
                     YYLQYPHLFRFIWLRNYLKLLEDPLFSYSLRFTLGYALVAVVVQVGLGLAVALLLNSR
                     IIGRGVIRGMMLMPLMMPPVITALMWKIMMASTKAGILNYLLSFLGLGPVNWLGAMNS
                     AIVSVFIIDTWGNLPFVAIILLGGLQSLPTEPYEAARVDGAAGWGVFRYITLPLLKPF
                     IILAATFRVMDSLRIFDVIYAATVGGPADATMNLHMRAFFYAFQWYQMGMGMAYAMVL
                     LILVFLASYLLMGYWRRAAEQTSL"
     gene            6996..7871
                     /locus_tag="VEL68_17970"
     CDS             6996..7871
                     /locus_tag="VEL68_17970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ37916.1"
                     /translation="MKTLSFGLTFWRWTRRVLLAVAVMALVAWTIFPFFWILLTSLKQ
                     PLDVIAAPPKVFFEPTTQNYQAIFIGQQRGMYASARPDFPNFFLNSIIISCGAVGLSF
                     LAGIPASYALARYSFRLKEQLAFLFLSFRFAPFIAFLIPLYVLFQQLNLYNTYTGLIL
                     AYQLISLPFTVWMLRSFFMEIPPEVEEAAKIDGCSWWGVLTKVILPLSLPGIAVTIIL
                     GFMFCWNAFNYPLMLAGRQTFPVTVGAIQFISYEQVLWGQMAAASLVAVVPQLILSLF
                     VQKYIVRGLTMGAIR"
     gene            8025..9005
                     /locus_tag="VEL68_17975"
     CDS             8025..9005
                     /locus_tag="VEL68_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ37917.1"
                     /translation="MSRTQITRRTFIKGAGLVAVAVAGRMGIPGTAAEASSAEHKVPH
                     SSGTEAPKLKAPAKACDCHLHIYDPRFPPPGPASRLMTNAGVEQYRLLQNRIGTTRAV
                     IVTPSAYATANEVTTDGISQLGPAHARGIAVVRSEVSDAELKKLADGGIRGIRFTLFD
                     PKTAVTTFEMIEPLAKRANDRGWHVQLHFRGDQIVEKEDLLKRIVAPVVFDHMGRIPQ
                     PAGVKHPAFQIICRLIDRGRTWVKLSGVYQDTKVGPPTYADATEVARAYLKFAPERMV
                     WGSDWPHPTEREHKPNDAVLFDLLTEWAPDEALRKKLLVDNPAALYGFGK"
     gene            complement(9186..10223)
                     /gene="argC"
                     /locus_tag="VEL68_17980"
     CDS             complement(9186..10223)
                     /gene="argC"
                     /locus_tag="VEL68_17980"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXZ37918.1"
                     /translation="MTRVGVVGATGYTGIELVRLLSRHPKVRLTALTAEKNVGQPIWK
                     LFPSVLKEVDLICQPLEVEPLSKTCDFVFTALPHKAVMEVAPGFLEKGIKVVDLSADF
                     RLADPKVYEQWYETHTAPELLKEAVYGIPELHREEIKKARLVANPGCYPTGVILALAP
                     VLKHKAIDIRTIIADSKSGVSGAGRSAVLSSMFAEVSENFKAYKVTEHRHTPEMEQDL
                     SRVAGEKVIITFTPHLVPMKRGILSTIYATLAKLRSEKEIYEMYLDFYGKEKFVRVRP
                     SDLLPSTADVFGSNYCDIAVKVDKRINRLIIISAIDNLVKGASGQAVQNMNVMLGLEE
                     SLGLDIIPVYP"
     gene            complement(10299..10691)
                     /gene="rpsI"
                     /locus_tag="VEL68_17985"
     CDS             complement(10299..10691)
                     /gene="rpsI"
                     /locus_tag="VEL68_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXZ37919.1"
                     /translation="MPEKVYYATGKRKTSIARVWMKPGDGKVTVNEQDLNQYFKLDTA
                     KMIIFQPLELTGTLGQFDIAITVQGGGMKGQAGAIRHGITRGLLAIKNEFRESLKKAG
                     LITRDPRKKERKKYGQKGARKRFQYSKR"
     gene            complement(10703..11131)
                     /gene="rplM"
                     /locus_tag="VEL68_17990"
     CDS             complement(10703..11131)
                     /gene="rplM"
                     /locus_tag="VEL68_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXZ37920.1"
                     /translation="MKTPLAKKENINPKWHLVDADGKTLGRLATRVAILLRGKNKPIF
                     APHQDTGDFVVVINAKKVALTGKKWKEKVYVHHSGYPGGLKMASAEKIAEKKPERLIT
                     MAVQGMLPKTKLGKKLIKKLKVYAGNAHPHEAQQPEAYTL"
     gene            11308..13779
                     /locus_tag="VEL68_17995"
     CDS             11308..13779
                     /locus_tag="VEL68_17995"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXZ37921.1"
                     /translation="MSDKGINQPKKLSLPIQGMTCASCAARIEKSLNALKGVREVNVN
                     LATEKANILYEPGLVNDRTLEKAVEDLGYQVLSDAGKEEKITVAIGGMTCAACVNRIE
                     KALRKIEGVSEAQVNLATEKATLLYRPDKVGKTEFRKTIEDLGYEIRGFEAEISADRE
                     AEARSLEVRIQKRKFLISAVLSVAIFFGSMPGWFPWWPKFLQNHFTLFLLTTPVQFWA
                     GWQFYRGFWLALKHRTSDMNTLIAVGTSAAYIYSAVVTFLPQWFRARGLDLGVYFDTS
                     AMIITLILLGKLLEAIAKGHTSSAIKRLMGLQAKTARVVRNGQEMDIPVEEVRKGDGV
                     IVRPGEKIAVDGIVREGNSAVDESMLTGESLPVEKHAGDSVMGGTINKTGSFKFEATK
                     VGKDTALAQIIKLVEEAQGSKAPIQRLADRVASVFVPAVISIALVTFAVWMIFGPKPG
                     FTLALLNFVAVLIIACPCALGLATPTAIMVGTGRGAENGILIKGGESLETIHRVNTIL
                     FDKTGTLTQGKPAVTDIVAEKGFDQSQVLEWAASAEKGSEHPLGEAIVRAAEENGLKL
                     KPVDAFEAIPGQGIKAKINGEAILLGNLKLMEENGVELDGIEGDLERLAGEGKTPMLV
                     ARNHSAAGIIAVADTLKPYSKEAVEALQRMGLEAVLVTGDNERTGKAVAAQMGINRVL
                     AEVLPWEKSQQVKRLQEEGKVVAMVGDGINDAPALAQADVGIAIGTGTDVAMESSDIT
                     LITGDLRAVVTAIQLSKRTIRTIRQNLFWAFFYNVLGIPIAAGILYPIWGILLNPMIA
                     SAAMAMSSVSVVSNSLRLRRFRPHI"
     gene            13976..>14815
                     /locus_tag="VEL68_18000"
     CDS             13976..>14815
                     /locus_tag="VEL68_18000"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ37922.1"
                     /translation="MEILFRNGNILTMEPGLPKAEALAVQFGKVLGIGRNEDMERLAG
                     PGTTVVDLQGQTALPGFIDTHSHFCLYALLTDQADCRPAAGCRKGEDVVEAIRAQAKK
                     TKPGKWIMGWGYAPYLLDDQKDLTREDLDRASTKHPICLVHVSVHGCVVNSLALKELG
                     FTRKTPNPPGGVINRDEKGNPNGILSESAFMGPLFFAAPSIYSKMMAEYDRKGRVEMI
                     SRCASRFHRLGIVGAHDPFVDAATLRTYQDAEMAGEFAFRLYAFILNQWADPLLAAGI
                     KRGF"
BASE COUNT         3416 a         3906 c         4247 g         3246 t
ORIGIN      
        1 agagaccccc aggtcgttca gcgcaagatg gtcaccgagt tcgtcgatcc gaggaaagga
       61 aagatgaagc tcctctcttc cccgatcaaa atgtccgaga ctcctcccga tattcgcagc
      121 gctcctgctg ttttcgggga gcacaccgag gaaattctga aggaactggg cttccgagag
      181 gaagagatcc ggcatatgaa aaaggaaggg gtggtttaac ggcagttcag gaattgccca
      241 gctgtggctt ttggtttttc tttcacagaa agggcggtga atatagtacg aatcatcagc
      301 ccgcgccggg cggtgaatca aaagcgggaa gattgttaaa aaaccaatgg ccctaagagg
      361 ggagccgcaa cgataaagga actgcagctc ccttgacaca ccattgcctt tctcatatat
      421 ttaaccgacc cgaaagcgag ttcttatcaa ttcaattctt gagaagaaga aaggagtaga
      481 gaaattgaac attcgagaac agagaatacg gaatctattc aatcgagaaa cgggaaaagc
      541 ggtgatcatt ccaatggatc acggcatcca gggggtcagg gaaggtctgg aagatcccct
      601 cggatcgttt cagaagttcg cgggattatg cccggaggcg gtgctgatga actttggcat
      661 cgttaaatta gtgcagaata ttatcagcgg ggccaccgac cccccgggga tcattatgac
      721 cacggatttt aaccaccgat ggcatagttg gaaggcgcca atcgaagaaa agggattatt
      781 gggtcattgc attagcgccg atgtcgaaca ggcggcccga tacaatgtag acgcggtaaa
      841 ggtattattt gatctcgggt tgaatcccaa gacccaactg tcggtgttta aaaatatcag
      901 cgaactcgtg cgtcaatgtg atcggtacga tatgcccatc atgattgagc ctctggcgga
      961 cggcctcttt atccccagcg aaaagaagaa tcatccaaag attatcgccg atggctgcag
     1021 gattgcgcta gagcttggag cggatataat taaggcggct tatcccggtg aaggacccga
     1081 atccaaggaa cagtttgcgg ccatttgcaa aaatgcccct gtgccggtgg tcatcctggg
     1141 tggcgccaag aaagacagca tccgtgaaat ctttcagatc gctcgagacg gtatcgacgc
     1201 gggggcgaaa ggggtaattt ttggaagaaa cgtatggcag cggccgaccc atgcgatgga
     1261 acgcgtcgta aagggattgc aggatatcgt tcaccgaaat gccggggtac aggaatcctt
     1321 atccaaatac aaccttcccg gggagaaatg aaatgaagag atcatagcgg aagtcagttt
     1381 acttcggaaa gtaggaggtt ttatgccaga aaagaccagg ttcggggtaa ttgtctctga
     1441 tcgactagca gaagttcatg agcacaaaat cccttcgctt ctttctacag aagtcctcat
     1501 cgagaataaa tcctgtaaca tttgtacaac ggattatcag caatggatgg ggcttcggtc
     1561 tcagcagccc acgcccatgg ccttcggcca tgaaaattgc ggcatcgtga tagaagtggg
     1621 aagtgaagtt gaaaatgcca gggtgggaga tcatgcggtg ataaataatt atcgtccctg
     1681 catggaatgt ggggactgtc gaaaaggaag aaatttcgtt tattgtaaat ttcgaaaaag
     1741 cgccagaacc ggcccggccg atgcgtatgg atattttgga aactatggct gctcgagata
     1801 ccaaattgcg aaatccaagc atatctttaa ggtaaataag gatgtgcctt ttgaactcgc
     1861 agggtttgcc gaaccgctat cgaccgttgt ctatggcata aaaaggcttc gggtgaaagc
     1921 aggggaaaag ctcctggtta ttggcgcagg caccatgggg aatctcaacg cccaggttgc
     1981 aagatatttc ggagctgacg tgaccctatc cgatgtatcg gaaaagaagt tgaagaccgc
     2041 tcaacggttg cgttttgaaa gaactttaaa tgcacgggat acagactatc ttaagaaagc
     2101 gagggcgctt acggacggcg caggatttga ttccatcatc atcgctgttg ggctcaccga
     2161 agcatacaat cacgccatcg aaatggtcgc cgacgggggg aagctgctta tttttgcggc
     2221 aggatacccg aagccgggat ggaatatcga tccgaatacg gtccattata aattgtggga
     2281 aatcatcggg acgtttggct gcgaggccgc ggattttcaa gaggctgtgg gtctgttgag
     2341 cgacaaaagg atcgacttgg cccctttaat tgaggaccgg atacctttga aggaaatcca
     2401 gcacgccttc gaaaaggcct cggcacctga tagctaccgg gtcgctgtct tactgggaga
     2461 ttcctaacag ggtgcgtaaa aactcgattt aggcagtcat tgcgagccac cgaaaggagc
     2521 gcggcaatct aatcatgcat gaatttatta tggatgagat tgcttcgctt cgctcgcaat
     2581 gacgctcaaa ggagttcttc agcatcctcc cagactgcgg gggtattgaa gttacctgcg
     2641 agaagtttca ttatatcctt ggcccagaag ggatgcttcc aagatgcgtt acgtgttggg
     2701 tgtagacata gggacgcaag gtataaaagg cgtgttattg gacgaggcgc ttaacgtcgc
     2761 ggcaaaggct taccgggaac atgattatat ccaaccgagg cccgactggt ttgaacatga
     2821 tgccgagaaa acctggtggg gaggctttaa agagattgtg catcaactgc tcaaaaaagc
     2881 gccgatttca tcaagcgaga ttatcggcgc cggttatagc gcgatcgccc cctgcatgct
     2941 gcccgtcgat gaagacggcc gcccgttgcg aaatgccatt ctttatggcg tggacacgcg
     3001 aagcaaaaaa gaaatcatag agatcacgca gatgctggga gaagaaaaaa tcctcgaaac
     3061 cgcgaaaaga ccactcggta ccgatctcat tcctccgaaa atgctctggt ataaaagaaa
     3121 cgaaccggac aaatattcga aaacgaagcg aatatttacc gcttctaatt atattaccta
     3181 caagctcacc ggccgcttta tgctcgacta tacgcaagcg gccggattcg gtccgattta
     3241 tgatttcggc aaaaagcagt ggaacgggga gatgtgtgaa ctgctcggct tttcttcgga
     3301 tctgctgccc gaactgaaac actgctatga catcgccggc acggtcacgc ctcgtgcatc
     3361 ggcggaaacc ggattgatgc aggggacacc ggtcgtcgtc ggttcgggag attatgctac
     3421 ggagctgatt agtgccggag gatttggccc aggggaagta acgttaattt acggcacaac
     3481 cgctattatt tccatgatga cggatagagc tccctccgta aaagggctgt ccgttacacc
     3541 ccatccgata ttggaaaact gcttttttgt aaatggcgga acggccacga tgggtgcctt
     3601 gacaaaatgg tttagggata atttcggcga tctggagaaa atcatgcagg atagaaccgg
     3661 gatcaatgcc tacaccctgc tgagctgcca ggcggaaaaa attccggtag gtgcggaggg
     3721 actggttatc ttgccgtatt ttagcggaga aagagccccc atacacgacc cattggccaa
     3781 aggggtcata ttagggctta ccacgtacca caaacgcgcc catttgtatc gggcgttgtt
     3841 ggaagggacc gcttacagcc ttcggcataa ttttgaggtc ttcgataaat gcgggtttga
     3901 cgtgtctcga gtgatcgcct gcggaggagg ggcaaacagc aacctgtggg tccagatcgt
     3961 cggtgatgtc atcggctatg accagtcgat cccgaacata cccattgggt cggatatcgg
     4021 gtctgcctat cttgctgcca aaggaacggg acttatcgca gacctcgcct ctttcatcac
     4081 caatcgtcgg caaaaggatg ctcgaaccgt gcgggccgac ccgcagaacc atgctcgata
     4141 tacggattat taccggattt atcgcaggct ttacgagagc gtgaaatccg acatgcacgc
     4201 gctggccgag ctggcggaaa atcatatggg caaaatggcg gaagggagtt tccggctggg
     4261 tgaggccgac cggtgaggtg actcggctcg gtagatgagc agataggctc caataaccaa
     4321 acagaaaggg gtagggaaag gaaaaaattg ctgcagttcc attgccatga acaagcaccc
     4381 tttcacggaa gcgttgttgc ccctcgcccc gcagttcgag cagctgacgg gcatgaaggt
     4441 gaagtatctg atcttaccgg aggaagaata cttccaaaaa atcgaggtgg ataggctgca
     4501 aatagaatgt ccttgacttt agttaaggcg cggatataat tcaggcgaat taaggaggat
     4561 gaatccctga aaggaggtaa ggcgatgaag caccggatgc gaacgaggcg gtggatgata
     4621 gcggcggctt tccttatcct ggggggatgg atctgctggg cagcggtgga ggcgttggcc
     4681 gcggaacctg atctgaccta tttccgccag gccaagatca actggcgtca attcgagggc
     4741 cagaagctga ccatcgggct gaacaagcac ccctataccg agtcactgct ccccctcatt
     4801 cccgaattcg aggccctcac gggaatcaaa gtcgaatacc tgatcctccc ggagaacgaa
     4861 tattttgcca agctcgtcgc cgacctgtcc aacgcacgcg gggagttcac cgtgatcatg
     4921 acgggcccca tgcgcaattg gacgtacgcc gctccgggct ggattctccc gctggaacca
     4981 tttctgaatg accccaagct gacggacaag gcctggtaca agccggaaga ctttttcccc
     5041 ggccttatcg cggctaaccg gtggaacggg aagatcggcg gtgggaccgg cgaaggggcg
     5101 ctctggtcga tcccgatcat gtgcgaaagc tatatcctgg cctaccggaa ggacatcttc
     5161 gataagtaca agatcaaggt gcccacgacc tatgaggagt tggccgaggc ggcccgtctg
     5221 gtaaagaaga acgcggggat cgacggagtc atggctcgcg gtacaccgaa cacgccaacc
     5281 atctccaccg ggtacatcag cggggcgaaa tcgtacaccg atgggaagtg ggccgaggcg
     5341 gaccaaaaca tgatcgcccg cctatccgac ccgcggcatg tcaatttcag caagatatgg
     5401 atcgatatgg tccgcgagag cgggccggcc aactgggcca acatgacctg gtacgacggt
     5461 atggagacct ttgccgcggg ccaggccggg atgtacccgg actgtgactt ctttgcccag
     5521 acctacgagg accccaaaaa gtcgaaggtg gcaggaaagg taggctatgc cctcctgccc
     5581 ccgggtcccg gcggtcgcac gtactcgggc ctctggacct gggcgctcgg catgagcaaa
     5641 gccaccaaga acaaggaggc cgcctggtac ttcatccagt gggccacctc tcagcggacc
     5701 ctcctgaacg ccacggtgga gtttcggaat tacaacccct cccgggcctc ggtcatgaat
     5761 gacccgcgga cgcaaaaggt tttggggagc tggggcaacg gggcctacgt gaagacggtc
     5821 caggaaaatc tcaagaccgc gcgggtggcc tgggttccca acccggaacg cggcaggcta
     5881 ggagacattt gggcccgggc cctgcacgag atctacttca agcggctctc cccggaggag
     5941 gctatgaagc gggccgatga agagatcaac aaaatcttca aagacatcgg gttgcagaag
     6001 tagcctctta ggcgatgaac catgccgctt cgaccgccgg ggcggcatgg ggttttttga
     6061 ataaaggtga agcacgaacc gtatgtcgag gccccatgcg ccagcgcgcc agcgcgatgc
     6121 catcctgccg tacctcctgc tttcgcccgg gttgctcttg ctcctgggaa tgttgtatcc
     6181 cttcttcctg ggggtctact atagccttac gaactactac ctacagtacc cgcacctctt
     6241 ccgcttcatt tggctgcgaa actacctaaa gcttttggag gatcccctct tttcgtactc
     6301 cttgaggttc accctgggct acgcgctcgt cgcggtggtc gtgcaggtcg gtctgggctt
     6361 ggcagtggcg ctcctgctca acagccgaat catcggccgg ggggtgatcc gcggcatgat
     6421 gctcatgccg ctcatgatgc cgccggtcat caccgcgctc atgtggaaga tcatgatggc
     6481 ctccaccaag gcggggatcc tcaattacct tctctcgttt ctggggctgg gaccggtcaa
     6541 ctggctcggc gccatgaatt ccgccatcgt ctccgtcttc atcattgaca cctggggaaa
     6601 cctgcccttc gtggccatca tcttgctggg ggggttgcag tcgctgccca cggagccgta
     6661 cgaggcagcg cgggtggatg gggccgccgg ctggggggtc ttccggtata tcacccttcc
     6721 cctcctcaag ccattcatca tcctggcggc gacctttcga gtcatggact cgttgcgcat
     6781 cttcgacgtc atctatgcgg ccacggttgg agggcccgcc gacgccacga tgaatttaca
     6841 catgcgcgcg tttttctacg ccttccagtg gtaccagatg gggatgggaa tggcttacgc
     6901 gatggtgcta ctcatcctgg ttttcctggc gagttacctt ctcatggggt actggaggcg
     6961 ggccgcagaa cagacatccc tatgaggaac cgatcatgaa aactctttca ttcggtttaa
     7021 ctttctggcg atggactcgg cgcgtactct tggccgtggc ggtgatggct ttagtggcct
     7081 ggactatttt ccctttcttt tggatcctgc tgacttccct gaagcagccc ctggatgtga
     7141 tcgccgcgcc gcccaaggtg ttcttcgagc caacgactca gaactaccag gccattttca
     7201 tcggtcagca gcgcggaatg tacgcctctg cccgaccgga tttcccgaat ttcttcctga
     7261 atagcattat catttcctgc ggggcggtgg gcctctcctt cctggccggg atcccggctt
     7321 cgtacgccct ggcgcgttat agcttccgat tgaaggagca gctggccttt ctcttcttga
     7381 gcttccggtt tgccccgttc attgcctttc tgattccgct ctacgtcctc tttcagcagt
     7441 tgaatctcta caacacctac accggcctga tccttgccta ccagctgatc tcgctgcctt
     7501 tcaccgtctg gatgctccgc agcttcttca tggaaatccc cccggaggtg gaggaggcgg
     7561 ctaagattga cggatgttcc tggtgggggg tcctaaccaa ggtgatcctg cctctcagcc
     7621 tgccgggcat agccgtaacg atcattttgg gtttcatgtt ttgctggaac gccttcaact
     7681 atcccctcat gctggcggga cgacagacgt tcccggtcac cgtgggggcc atccagttca
     7741 tctcctacga gcaggtgctg tgggggcaga tggccgccgc ctcgctggta gcggtcgtcc
     7801 cgcagctgat tctcagcctc ttcgtacaga agtacatcgt ccgcgggctg accatgggcg
     7861 cgattcgctg aagcaggagt gcagggaagg gaaagcagct agcagttcac cgaatgtata
     7921 gttgaggcta atatactcga ggatagacaa gaataaacta tagataaaat tcgggaacag
     7981 gaccttaaaa aaacgagctc ggaaactata agaggaggta tgtcatgagt aggacccaga
     8041 tcacacgtcg tacttttatc aaaggagctg ggttggtggc cgtggcggta gcgggcagaa
     8101 tgggcattcc cgggaccgcc gcagaagctt cttccgccga gcacaaggtt ccccattcct
     8161 ccggcaccga agcgccgaag ctgaaagcgc cggcgaaggc gtgcgactgt caccttcaca
     8221 tctatgatcc gcgctttccc ccgccgggac cggcctcgcg gctcatgacc aacgcagggg
     8281 tggagcagta ccggcttctc cagaaccgga tcgggacgac gcgggcggtc attgtcacgc
     8341 ccagcgcgta cgccacggca aacgaggtca ccaccgacgg catttcccag ctgggcccgg
     8401 ctcatgcgcg gggaatcgcg gtcgtccgct cggaagtcag cgatgccgag ctgaagaaat
     8461 tggcggacgg ggggatccgc gggattcgat tcactctctt tgatcccaag accgcggtca
     8521 ccactttcga gatgatcgaa cccctggcca agcgggcgaa cgatcgcggc tggcacgtgc
     8581 agctccattt ccggggagac cagattgttg aaaaagaaga cctgctgaag cgcatcgttg
     8641 cgccggtggt gttcgatcac atggggcgca tcccccagcc agcgggggtg aaacaccccg
     8701 ccttccagat catttgccgt ctcatcgacc ggggacgaac ctgggtcaag ctttcgggcg
     8761 tctatcagga caccaaggtc gggccgccca cctacgcgga cgcgaccgag gtcgcccgcg
     8821 cctacctgaa gtttgcaccg gagaggatgg tatggggaag cgattggccg cacccgaccg
     8881 agagggaaca caagccaaac gacgccgtgc tcttcgatct gctgaccgag tgggccccgg
     8941 atgaagcctt gcgcaagaaa ctcctcgtcg acaatccggc agcactgtac gggttcggga
     9001 agtaggcgaa atcgacgttg ctggttacga gtgcctgctg gaggcggatg gccgatggcg
     9061 aaattagaaa cgggcgagga ataaccaata ccgggaggca tgtttcatgc tcggaaatcc
     9121 ttaggggcga ccggccggtt gcccctacaa accccgaact ctgaactaaa ttactccgga
     9181 ctctattacg gatataccgg tataatatcc agccccaggg attcctcgag gccgagcatc
     9241 acgttcatgt tctgcacggc ctggcccgag gctcccttga ccaggttgtc gatggccgaa
     9301 atgatgatga ggcggttaat ccgcttgtcc accttcaccg cgatatcgca atagttggag
     9361 ccgaacacat ccgcggtgct ggggagaagg tcggagggac gcacccggac gaacttctcc
     9421 ttcccgtaga aatccaggta catctcgtag atttcctttt ctgaccggag tttggccagg
     9481 gtggcataga tggtggagag gattccccgc ttcatgggca ccagatgagg ggtaaaggtg
     9541 ataatgacct tttccccggc cacccggctg aggtcctgtt ccatctccgg ggtgtgccgg
     9601 tgttcggtca ccttgtaggc cttgaagttc tcgctcacct cggcaaacat ggaagagaga
     9661 acggccgacc ggccggcgcc gctgaccccc gatttagagt cagcgatgat cgtgcggatg
     9721 tcgatggcct tgtgtttaag gactggggcc agggcaagaa tcacgcccgt gggatagcag
     9781 ccggggttgg cgaccagcct ggccttttta atttcttccc ggtgcagttc gggaatgccg
     9841 taaaccgcct ccttcaaaag ctccggtgcg gtgtgggttt cataccactg ttcatagacc
     9901 ttcggatcgg ccaggcggaa atccgcgctc aagtccacca ccttgatccc cttttccaga
     9961 aagcccggcg ccacctccat gactgcctta tggggcaaag cagtgaagac gaaatcgcag
    10021 gtcttggata agggctcgac ttccaggggc tgacagatca ggtccacttc tttcaaaacg
    10081 gacgggaaga gcttccagat cggctggcct acgtttttct cggcagtcag ggcggtcagc
    10141 cgcaccttgg ggtgtctgga aagcagccgc accagctcga tgccggtata tcctgtcgct
    10201 ccgactacgc caactctcgt catggtcctt ctccatgaaa aaggcagtaa aaaagggaag
    10261 gccggaagac cttcccttgg tttttactcc aagccggatt accgtttgga gtattggaat
    10321 ctcttccggg cgcctttctg gccgtacttt ttacgctctt tcttccgggg gtcccgggta
    10381 atgaggccgg ccttcttcag ggattctcga aattcgtttt tgatagcgag gagcccgcgg
    10441 gtaatcccgt ggcggatggc tccggcctgt cctttcatcc ccccgccttg aaccgtgatg
    10501 gcaatgtcaa actggcccag tgtcccagtc aactccaggg gctggaagat gatcatcttg
    10561 gccgtgtcca gcttgaagta ctggttcagg tcttgctcat tcactgtgac ctttccgtca
    10621 cccggcttca tccagactcg agcgatggag gtttttctct tcccagtggc ataataaact
    10681 ttttcgggca taggttcttc ctttacaaag tatatgcttc aggttgttgg gcttcgtggg
    10741 gatgagcatt tccggcatag actttaagct ttttgatcag ttttttcccc agtttggtct
    10801 tgggcagcat tccctggacg gccatggtga tgaggcgctc gggcttcttt tccgcgattt
    10861 tttctgcgct ggccatcttc agcccgccgg ggtatccgga atgatgaacg tagaccttct
    10921 ctttccattt tttcccggta agggccactt tcttggcatt gatgacaacc acgaaatcac
    10981 cggtatcttg atggggggca aagattggct tgttctttcc ccgcagcagg attgccaccc
    11041 gcgtggccag ccgccccaaa gtcttgccat cggcatcgac caggtgccat ttggggttta
    11101 tattctcttt ttttgccaga ggggttttca tatacggacc tcagttgtaa attaaattta
    11161 aattatatag aattaaatct accatttcaa cggaaaaatg cagggggaga aatcgttctt
    11221 gcattcagcc acgcctactt tgcattttac cctaattttc aaaaatttat gttgaatcaa
    11281 agggttctaa gccgcatcta aaatattatg agtgataagg gtataaatca gccgaaaaaa
    11341 ctatctttac ccatccaagg tatgacctgc gcctcctgcg cggcgcgaat tgagaaatcc
    11401 cttaacgcgc tgaaaggggt ccgggaggtg aatgtcaatt tggccacgga aaaagccaat
    11461 attttgtatg aacccggctt ggtgaatgac cggaccctgg aaaaagccgt cgaagacctc
    11521 ggctaccagg tcctttcgga cgcggggaag gaagaaaaaa taaccgtagc catcgggggc
    11581 atgacttgcg ccgcctgcgt gaatcgaatc gagaaagccc tacgcaaaat cgaaggggtg
    11641 tcggaggctc aagtcaatct ggcaacggaa aaagccactc tcctttaccg gccagacaag
    11701 gttgggaaaa ccgagttccg aaaaaccatc gaagacctgg ggtatgaaat ccggggtttc
    11761 gaggcagaaa tttcggccga ccgggaagcg gaagccagaa gccttgaggt tcgcatccaa
    11821 aaaaggaagt tcctcatcag cgccgtcctt tctgtggcca tctttttcgg cagcatgccc
    11881 gggtggttcc cctggtggcc taaattcctg caaaatcatt ttaccctctt cctgctcaca
    11941 actccggtcc aattctgggc cggctggcag ttttaccggg gtttctggct agccctgaag
    12001 caccggactt cggacatgaa caccctcatc gcggtgggaa cctccgccgc ctacatctac
    12061 agcgcagtgg tgaccttcct cccccaatgg ttccgggctc ggggattgga cttgggggtt
    12121 tattttgata cttcagccat gatcatcacc ctgatccttt tggggaagct tctggaagct
    12181 atcgccaagg gtcacacatc ctcggcgata aaacggctca tgggtcttca ggccaagact
    12241 gccagggtgg tccggaacgg gcaggagatg gatatcccgg tggaagaggt acgcaaaggg
    12301 gatggggtga ttgttcgtcc cggggaaaaa atcgccgtcg atgggattgt ccgggaggga
    12361 aattcggcgg tggacgaatc catgctgacc ggagagagcc tccccgtgga gaagcatgcc
    12421 ggggactcgg tcatgggggg gacgatcaac aaaaccggtt cctttaaatt tgaagcgacc
    12481 aaagtgggca aggacacggc tctggcccag atcatcaaac tggtggaaga agctcaggga
    12541 tcgaaagctc cgatccagag gctggcggac cgagtggcga gcgtctttgt gcccgcggtc
    12601 atatccatcg ccctggttac ttttgctgtc tggatgattt tcgggcctaa gccgggtttc
    12661 actctggctc tgctcaattt cgtggcggtc ttgataattg cctgtccctg tgctctgggg
    12721 ctggcgaccc ccacggccat catggtggga actggccggg gagcagagaa cggaatcctt
    12781 atcaaaggag gagagagctt ggagaccatc caccgggtga acaccattct cttcgacaaa
    12841 accggaacgc ttacccaagg caaacctgca gttaccgaca ttgtggccga aaaaggtttt
    12901 gaccagagcc aggtccttga atgggccgct tctgcggaaa aagggtcgga acatcccctg
    12961 ggggaggcca ttgtccgcgc tgccgaagag aacggcctga agctcaagcc tgtggatgcc
    13021 tttgaagcca ttcccgggca gggaataaag gcgaaaataa acggggaagc gatcctcctc
    13081 ggaaacctga agctcatgga agagaacggc gtcgagctgg atgggattga gggagatttg
    13141 gagcgtttgg cgggggaggg gaagaccccc atgctcgtgg cccgaaatca ttctgcggcc
    13201 ggaatcatcg ccgtggccga taccctcaag ccttattcca aggaggcggt tgaggccctg
    13261 cagcggatgg gcctggaagc ggtccttgtt accggcgaca atgaacgcac gggcaaagcc
    13321 gtggctgctc aaatgggaat caaccgggtt ctggccgaag tccttccctg ggaaaaaagc
    13381 cagcaggtta agcgattgca ggaagagggg aaagtggtgg ctatggtcgg cgacgggatc
    13441 aatgacgcgc cggccctggc ccaagccgat gtgggaatag ctatcggtac ggggaccgat
    13501 gtggccatgg aatcttccga cattaccctc atcacgggag acctgcgggc ggtggtgact
    13561 gctatccagc tcagcaaaag gacgatccgt acgatccgac agaacctctt ctgggctttc
    13621 ttctacaacg tgttggggat ccccatcgcc gccgggatcc tctatcctat ctggggaatc
    13681 ctcctcaatc ctatgatcgc ctccgcggcc atggccatga gttctgtgtc cgtggtcagc
    13741 aattccttga gactgcggcg gttccggccc catatttaac ggcagattct ggataccgga
    13801 tactggatgc cagatcatta aatcatttct ttctatgaac cggcagctag agtccaggat
    13861 cagatttttt tctccaacct tgaccgcccc gcctatttct gatagaagaa ttactcatca
    13921 tcggatatcc agtagcaagt atccaatatc cagaatctag aaaggggagg ggatcatgga
    13981 gattcttttc aggaacggaa acatcctgac catggaaccg ggcctgccaa aggcagaagc
    14041 cctggcggtc caattcggga aagtcctcgg aatcggtaga aatgaggaca tggagaggct
    14101 ggccggtccg gggacaaccg tggtcgacct gcagggccag accgcgcttc ccggcttcat
    14161 cgacacccat agtcatttct gcctttacgc cctcctgacc gatcaggcgg actgccgccc
    14221 ggcggccggg tgccggaagg gggaagacgt ggttgaagcc atccgagctc aggccaagaa
    14281 gaccaagcct ggaaaatgga tcatggggtg ggggtacgcc ccctaccttc tcgacgacca
    14341 gaaagatttg acccgagaag acctggaccg ggcttcgaca aaacacccca tttgcctggt
    14401 acatgtgtcc gttcacggat gcgtggtcaa ttcccttgcg ttgaaggaac ttggttttac
    14461 cagaaagacc ccgaatcctc ccggcggggt gataaaccgg gacgagaagg ggaatcccaa
    14521 cgggatcctg agcgagtcgg cgttcatggg gccccttttc tttgctgctc catccattta
    14581 ttccaagatg atggcggagt acgaccgcaa ggggagggtg gaaatgatat cccgctgcgc
    14641 ctcccggttc catcgcctgg gaatcgtagg cgcccacgac ccctttgtcg atgcggcaac
    14701 cctgcgaact taccaggatg ccgagatggc aggagaattc gccttccgcc tctacgcttt
    14761 catcctgaat caatgggcgg accccctcct ggccgcgggc atcaagcggg gattc
//
LOCUS       DASPRR010000490         9637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_604975, whole genome shotgun sequence.
ACCESSION   DASPRR010000490 DASPRR010000000
VERSION     DASPRR010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604975"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..903
                     /locus_tag="VEL68_18005"
     CDS             478..903
                     /locus_tag="VEL68_18005"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXZ37923.1"
                     /translation="MPIYVETFRATVAPADCDHLGHMNVQHYFRAVGDGMFAAMARIG
                     LTPEAIRRRKLSFAVVRAETEFHHELYAGDVIALESTILKVGKKSATFHHRLKNAATD
                     DIVMSTEFKCVLLNLENRQAALVPDDIRNAAVHSFSSGM"
     gene            1180..1596
                     /locus_tag="VEL68_18010"
     CDS             1180..1596
                     /locus_tag="VEL68_18010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003207338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ37924.1"
                     /translation="MTIEHYEEVLALMGRTPGVTIRDADSREATRRYLERNPSLSFVA
                     TENGRVIGCAMCGHDGRRGYLQHVIVEPAFRGQGIAHQLVTRCLDQLEAIGICKTHID
                     VFRTNDIANAYWARRGWKKRDDIYRYSYNRSRNENA"
     gene            complement(1821..2318)
                     /locus_tag="VEL68_18015"
     CDS             complement(1821..2318)
                     /locus_tag="VEL68_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HXZ37925.1"
                     /translation="MNNIRGRVWKVGDQINTDLITPGQYLTAPIEEVKKHVLEAVNPL
                     LAKEVRQGDILVAGKNFGCGSSRESAPRALKALGISAVVAESFARIFFRNAVAIGLAV
                     VPCPGVSQSLEDGEMLELDLESARVKNLNRKIQLLGNPLPKEMLDVLNKGGITPLLKE
                     MFGKS"
     gene            complement(2534..3790)
                     /locus_tag="VEL68_18020"
     CDS             complement(2534..3790)
                     /locus_tag="VEL68_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXZ37926.1"
                     /translation="MGMTLAEKILARASGRSTVKAGDYVVASIDLAMIHEGMRGVYPI
                     LKEAGVKKLWDPEKVVSIIDHWVPAPTVEAAVHHQTVRKAVEEYGIRHAYGEKAGICH
                     QVLPEKGHVVPGDLIVGTDSHTVTYGAFGAAATGIGYSEMAYVLATGKLWFRVPETLR
                     FQMDGALPKGVMSKDLLLYIAGRYSAEVAQYKAIEFTGSTAREMSVASRMTMSNMSVE
                     LGAKFGFFEPDDKVVDFLNGRAKKAYSLIQPDLDAIYESVHRLDASILEPQVAFPHNI
                     DNVKSIYGVERVKIDQAFIGSCTNGRIEDLRVAAQILKGKKVHPQVRLIVVPASWEVY
                     REALGTGILETLVDAGALICNSTCGPCFGGHMGLLAPGERCIASINRNFQGRMGSPES
                     EVYLGSPATVAASAVEGVIADPRNYL"
     gene            complement(3866..5239)
                     /locus_tag="VEL68_18025"
     CDS             complement(3866..5239)
                     /locus_tag="VEL68_18025"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXZ37927.1"
                     /translation="MEVTKKLSEFIINTDLGVIPPEAREMGKRVILDLLGVALAGSRD
                     PMARIMTDYIKDTGGRPQASVWGKKFKTSSSLAALANGTFGHALDYDDINRNMRGHPT
                     VPVLPAAMAAGEEGKTSGKEVLEAFIIGLEVETKLGSGMNPYLFENGWHPTAILGAMG
                     AAAAAAKLFKLPGDKVCMALGIAGSLASGLRQNFGTMTKPLHAGRAAQNGVTAAQLAR
                     RGYTADPGIVEAKLGYANAFSGAGKFDLNKIIANLGRPFDIVSPGVGLKRYPSCARTH
                     PAIDAMLDLAVQNDLRPEDVESVACAGTYTTPTMLIHSRPRTALEGKFSMEFCMALAL
                     MERKVTLPDFRDQKVQDPGIQDLIRKVTFSIRPDLNTIEHSGNPSTTVKVTLKDGREF
                     AKTVDEARGTPGNPLTAGEVKDKYRQCVKGIQSKKDMEKTIELVEDLENLKKTSTLAD
                     LLRGQKK"
     gene            complement(5398..6621)
                     /locus_tag="VEL68_18030"
     CDS             complement(5398..6621)
                     /locus_tag="VEL68_18030"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ37928.1"
                     /translation="MADGKKESSGPLKGVRIIDLSMMLAGPSGSMLMGDLGAEIIKVE
                     PLDGDETRVGPPYFYAENMSAYYWSINRNKKSIVINLKSPEGRQVLYDLVAKSDVVYD
                     NYRPGVLERLQIDYETLKKYNPKIICCSISTYGYNGPYRDRPGYDLIVQAVSGGMSIT
                     GEPGGSPVRAGIPLGDLVGGLLGVHGVLAAYIHRQKTGEGQRLEVSLLDGQVYLLTYI
                     AQYYFHSGRVPGPIGAGHQSLVVYQAFKTKDIQIVIVAHQDHHFQRLCKAIGKPEWAE
                     DPRFNTRTKRLENKSQLIPMMEAHLMTRPGDEWLKSIHQAGVPAGPINTLDRVLSDPQ
                     VLSRDMIQETDGPGKEKIKILGNPLKFDKTPVDTFTRPPRLGENTRGILSTILGYSAE
                     KIVGLEKKEAIKSGD"
     gene            complement(6730..8478)
                     /locus_tag="VEL68_18035"
     CDS             complement(6730..8478)
                     /locus_tag="VEL68_18035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXZ37929.1"
                     /translation="MSQNDSLKKVREEKEKWEGKTLKPALQRFKLKESPTRFYSPLDT
                     GEDFKFLEKVGFPGQYPFTAGTFPTFPYRTGERGSGSIAQAPGLARAGRYSGYGSAED
                     TRDYYLHMKKLGQKAGPNIAFDLPTQIGYDSDNPLAAGEVGKVGVAVDTLRDMEIIFE
                     AFSGETDLDRTASNFTINAPANIIMAMYLALADQRGISWDKLRATPQNEILKEYVARA
                     TYIFPPRHAMRMFRDSLVFFVKHVPNVNITSMGGFHIREAGATREQDLAYSMAIGAAY
                     LQEGVNAGLDVDDFAPRFSFNAFGGSMDFFKEIAFHRAARRMWAKVVKEKFGAKNERS
                     MLLRMASTAHCGRVNCSIQRPLNNLTRSIVGGIAAALSGGPPNCNPPFDEPLGLGWSL
                     EAIQLSEDAARILQNEARLTEVMDPLAGSYYVESLTDQVENAAWAEFDKIQSMGGVVK
                     AIESGYLQREVARSAYDRQKRLEEGQDLIIGVNCYTGESELEVQTTRLVAHPYDPDRR
                     ENAEKVQIERLKEVKRTRDSKKVTELLKKLKGEAAREEVNLFPTLIECVKAYATLQEM
                     CDVLREIFGEYQPAAL"
     gene            complement(8670..>9637)
                     /locus_tag="VEL68_18040"
     CDS             complement(8670..>9637)
                     /locus_tag="VEL68_18040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37930.1"
                     /translation="FRFFIFAFLVSLVTLVLPSPTSAQDYPSKPITIYCGYEAGATTD
                     LTARALGAGLEKLLGVPVVVENKAGGGATVAAGLVASRKPDGYTLGVVSTGVITVRPH
                     IFKVSYDPFKDFTLIAQYSRYIGGLCVLNDSPLKTIDDFIAYAKSKLGLSYGSSGAYT
                     QQHLAVELFSQCKGLNLKHVPYKGGAPANTALMGKHTDFVAGAGQHMQYVRQGVFRML
                     MVYNTDKRDPDYPDIPTLKELGCPDAPALGYILVAPKGIPEPVYEKLLTATRKIVEGA
                     EFQNLLKHLDLPYDFKDGKSMEKDVAAESAWYKEFLPKMGAKVIN"
BASE COUNT         2054 a         2730 c         2652 g         2201 t
ORIGIN      
        1 cggcaaataa cacggcttta agggggaaaa ccatgctaaa acccggattt ccttgcccac
       61 ccccagttct taaaaaagag tcctccgaaa ataagatctc cggaaaggag gggggatctc
      121 ttggggttca gggtggagaa atttggatca tcatcctgag gcggtggttc ttccatggga
      181 tcgggcctgg cctgggggga ttgaaacgga aaaatgcttt ggctctcttt tctgcttgga
      241 ggtatcccgt ctcaggcctc tgcctgcgga acatggggag gagtttcatg gaaggataag
      301 cacccgccga gcctgatgct ccaaattcct ccaccctgaa ccccgacaag catgaaaaac
      361 tgggggtggg caagcccggg tttctccttg attcgcccca tgcctcttga taaaatcacg
      421 gagaaaacag tcgctgcata aattattatt atgttagatt ccacgaaggg gacggccatg
      481 ccgatctacg tggagacctt ccgagccact gtcgccccag ccgactgcga ccatcttggc
      541 cacatgaacg ttcaacacta ctttcgtgcc gtaggagacg gcatgttcgc tgcgatggcg
      601 cgaatagggc tgactccgga ggcgatccgt cgccgtaagc tgtcattcgc agtcgtgcga
      661 gcggaaacgg aatttcatca tgaactctac gctggcgacg taatcgcatt ggaatccacg
      721 atcttgaaag tcggtaagaa gtcagccacc tttcatcaca ggctcaagaa cgcagcgacc
      781 gatgacattg taatgagcac ggagttcaaa tgtgtcctac ttaatcttga gaataggcaa
      841 gcagcgcttg ttccagacga tattcggaac gcggccgtcc actccttctc cagtggaatg
      901 taacttccag gtggagcggc ttgagctacc agaggaacag aatgaagggc ctgcgagcag
      961 taatcccatc gcaacattga acattcttaa ggcgcgctcc gtgtaaagaa ggcgctggaa
     1021 ggcagcgttg aacgctagct atacaaggca actcgcgagg cggccacgac aaacagcagg
     1081 ccaaaggaca gcgactgctt gaccgaaatt aattgtctct gcggttttat gaaaactgta
     1141 taatccttgt tgggcgaatg aactatgaac atcgtagaaa tgactattga gcattacgaa
     1201 gaggttctag cgcttatggg tcgaaccccg ggagtgacga tccgagatgc tgactcgcgc
     1261 gaagctacac ggagatatct ggagcgcaat ccttcgctga gttttgtcgc gacagagaat
     1321 ggaagggtta ttggatgcgc catgtgtggc cacgatgggc ggagaggata cttacagcac
     1381 gtgatagtgg aacccgcgtt tcgaggtcaa ggaattgctc atcaactagt tactcggtgt
     1441 ttggaccaac tggaggcgat aggcatttgc aagacgcata ttgacgtttt ccgcacaaac
     1501 gatatcgcca acgcttactg ggcgcggcgt ggatggaaga aacgcgacga tatctatcgc
     1561 tactcgtaca accgatcaag gaacgaaaat gcctaactgg gcggtggaac cctacgctgc
     1621 taacgcggcg cggttcaccg ccatcgttga cgccttcaat cgataatatt caccttttca
     1681 tcacggttcc gcgtatcgag ttggcggagt cgttattttt ggaagccgga tacaggtcct
     1741 atgaatgaaa attttcctgg agcaggatgg atatttgaga ttatttgcgt atattcactt
     1801 tgggctactc cgacccatga ttagcttttc ccaaacatct ctttcaatag gggagtgatc
     1861 cctcctttat tcaggacatc gagcatctcc ttcgggagcg gattccccag aagttggatt
     1921 ttccggttca aatttttcac ccgggcgctt tcgaggtcca actccagcat ctctccatct
     1981 tccaaagatt gggaaactcc gggacagggg actaccgcca ggccgatggc cacggcattg
     2041 cggaagaaga tgcgggcgaa ggattcggct accaccgcgg agattccaag ggccttcagt
     2101 gcccgcggag cagattcccg gctggagccg cagccgaaat ttttcccggc taccaggata
     2161 tctccctgcc ggacttcttt ggccaagagg gggtttacag cttccagaac gtgcttcttc
     2221 acttcctcga tgggggcggt aaggtattgt ccgggggtaa tcaagtcggt gttgatctga
     2281 tcccccactt tccaaacccg acctcggata ttattcattc tttccctttt tttttcacca
     2341 caaaggcgca gggaacacag tgccgcgcgg ccgcaaacaa atcgtagggg caaccccctg
     2401 tggttgcact tcctgcgggc ggccacaggg ggccgccccc acaatattct tttttggttc
     2461 catcttcccc ggatcaggac gatttccctt ttcgcgggct tggatttcta ttccgcaatc
     2521 cgcaattttt ttctcacagg tagttccgag gatcggctat caccccttcg accgccgaag
     2581 cggcgaccgt ggctggagag cccaggtaga cctcggattc ggggctgccc atccggccct
     2641 ggaagttccg gttgattgag gcgatgcacc tctctcccgg ggcgaggagc cccatgtgcc
     2701 ccccaaagca agggccgcaa gtggaattgc agatgagcgc gccggcatcc accagcgttt
     2761 ccaggattcc cgtcccaagc gcctcccggt aaacctccca cgaggccggg acgacgatta
     2821 ggcggacctg cgggtggact ttcttacctt tcaaaatttg agccgcaacc cggaggtctt
     2881 cgatcctccc attggtgcag gagccgatga aggcctggtc gatcttcact cgctcaacac
     2941 cgtagatgga cttcacgttg tcgatgttgt gaggaaaggc cacttgcggt tccaggatgg
     3001 aggcatccaa ccgatgtacg ctttcgtaga tcgcatccag gtctggctgg atcagggaat
     3061 acgctttttt cgcccgtcca ttgaggaagt ctacgacctt gtcatccggc tcgaaaaacc
     3121 cgaacttggc ccccagttcc acggacatgt tactcatggt catccgggag gcgacagaca
     3181 tctcccgggc tgtcgagccg gtaaactcga tggccttgta ctgggcaacc tccgcggaat
     3241 accgcccggc gatgtagagg aggaggtctt tggacatgac tcctttcggc agcgctccat
     3301 ccatctggaa ccggagggtc tcgggaaccc ggaaccagag tttgccggtg gccaggacat
     3361 aagccatctc cgaataaccg atgcccgtgg ccgcggctcc gaatgccccg taggtcacgg
     3421 tgtgggaatc cgttccaaca atgaggtcac cggggactac atggcctttc tccggaagaa
     3481 cctggtggca gattccggcc ttctccccgt aggcgtgacg aatgccgtat tcctcaactg
     3541 ccttccggac cgtctggtgg tgaaccgccg cctccacggt gggggccgga acccagtggt
     3601 cgatgatgct gaccaccttc tcagggtccc agagtttttt gactccggcc tctttcagga
     3661 tagggtaaac cccccgcatc ccttcgtgaa tcatggccag gtcgattgag gccacgacat
     3721 aatccccggc cttaaccgtt gaacggcccg atgcccgggc gagaattttt tcggccaaag
     3781 tcatgcccat aacatctaaa aaagtttcaa gttgcaggtt gcaagtttca ggtttaagac
     3841 gaaaccccaa acccgaaacc cgaaactact ttttctgtcc ccttaaaagg tcagccaggg
     3901 tggaggtctt tttcaggttc tccaggtcct ccaccagctc gatggtcttt tccatatcct
     3961 ttttggactg gatccccttc acacactgcc ggtatttatc cttcacctcc ccggcagtca
     4021 agggattccc cggggtgccc cgggcttcgt ccactgtctt ggcgaattcc cttccatctt
     4081 tcagggtgac cttcacagtg gtggagggat tgccggagtg ttctatggtg ttcaagtccg
     4141 gacggataga aaaggtaact ttccggatca ggtcctgaat cccggggtcc tggactttct
     4201 ggtccctgaa atcgggaaga gtgactttcc tttccatcag ggccaaagcc atgcagaatt
     4261 ccatgctgaa ttttccttcc agcgcggtgc gagggcggga gtggatgagc atcgtgggag
     4321 ttgtataggt tccggcgcag gcaacggact ccacgtcttc ggggcggagg tcattctgta
     4381 ccgccaggtc cagcatggcg tcgatggccg gatgggtccg ggcacagctg ggatatcgct
     4441 tgagccccac gccgggagat acgatgtcaa aggggcgccc cagattggct atgattttgt
     4501 tcaaatcaaa ttttcccgcg ccggagaaag cattggcgta ccccaacttg gcctccacga
     4561 ttcccgggtc tgcggtgtat ccccgccgag ccagttgcgc agccgtcact ccgttctgag
     4621 ccgctcggcc ggcatggagg ggcttggtca tggtcccgaa attctgccgc agcccggagg
     4681 ccagagagcc ggcaatgccc agggccatgc aaactttatc cccgggcagc ttgaaaagct
     4741 tggccgcggc ggcagccgcc cccatagccc ccaggatggc ggtgggatgc cacccgttct
     4801 cgaagagata gggattcatg cccgatccaa gcttggtttc cacctccagg ccgatgatga
     4861 aggcctccaa cacctctttc ccggaggttt tcccttcttc gccggcggcc atagccgcgg
     4921 gaaggacggg gacggtgggg tggccccgca tgttccggtt gatatcatcg tagtccaggg
     4981 catggccgaa ggtgccgttg gccagagcgg caagggagga ggaggtctta aactttttcc
     5041 cccagacact ggcctgcggg cggccgcccg tatccttaat ataatcggtc ataatccggg
     5101 ccatggggtc ccgagagccg gccagggcca cccccaacag atcgaggatc actcgtttcc
     5161 ccatttccct tgcctctgga ggaataacgc ccaggtcggt atttatgatg aattcagaga
     5221 gctttttggt cacttccata gctgcacccc actaaagaga atcaaatccc aaatcccaag
     5281 cacccaatcc ccaatcccaa ataccaaata ccaaatacca aataccaaat accaaataca
     5341 ttccaaaccc taatgttcaa agaccaaaac aatccttctt acagggggat ttggtgctta
     5401 atcacccgat ttaatcgctt ctttcttttc caaccctaca atcttttccg ccgagtatcc
     5461 caggatggtc gagagaatcc cgcgcgtatt ttctcccagc ctcggcgggc gggtgaaggt
     5521 atccaccggg gttttatcaa atttcagcgg atttcccaga attttgattt tttcctttcc
     5581 aggaccatcc gtctcttgga tcatgtcccg gctcaaaacc tgagggtccg acagaacccg
     5641 gtctaaggta ttgatagggc cggcgggaac cccggcttga tggatcgatt ttagccactc
     5701 gtctccgggc cgggtcatca gatgagcctc catcatgggg atcagctggg atttattctc
     5761 caggcgtttg gtgcgggtgt tgaaccgcgg gtcctcggcc cattcgggct tcccgatggc
     5821 cttgcagagc ctctggaaat ggtggtcctg gtgggctacg ataacgatct ggatatcctt
     5881 ggtcttaaag gcctgataga cgacgagcga ctgatggccg gctccaatgg ggccggggac
     5941 ccggccagag tgaaaatagt actgggcgat ataggtcaat agatacacct gaccgtccag
     6001 gagagagacc tccagacgct ggccctcccc cgtcttttgc cggtgtatgt aagcagccaa
     6061 aaccccgtgg acgcccagca gacctcctac caggtcgcca agggggattc cggcgcggac
     6121 cggcgagcct cccggctctc cggtaatgga cattcctccg ctcactgcct ggacgatgag
     6181 gtcatacccc gggcggtcgc gataaggacc attgtaccca taggtggaaa tggaacagca
     6241 gataatcttc ggattgtatt ttttcagggt ttcatagtcg atctgaaggc gctccaaaac
     6301 ccccgggcgg tagttgtcat agaccacgtc ggacttggcc accagatcat aaagaacctg
     6361 acgtccttcc ggggacttca ggttgatcac gatgcttttc ttattgcggt tgatggacca
     6421 gtaataagca ctcatattct ccgcataaaa ataaggaggg cccacccgcg tctcgtcccc
     6481 gtccaggggt tcaactttta tgatctcagc ccccaggtcg cccatcaaca tggacccgga
     6541 aggcccggcc agcatcatgc tcaggtcaat aatccggacc cctttcaagg ggccgctgct
     6601 ctcttttttc ccatccgcca tgttcgctct ttctcctctc ccgtattatg agtttcaagt
     6661 ttcaagtttc gggtttcgct gacttaaagc tattaacctg caacctgaaa cttgaaacct
     6721 gtgacctgtt tataaggctg ccggctgata ttctccgaaa atttcccgca ggacatcgca
     6781 catttcctgc agggttgcat aagccttcac gcattcgatt agagtgggaa aaaggttgac
     6841 ttcctcccta gccgcttcgc cctttagctt cttgagcagt tctgtcacct ttttggaatc
     6901 ccgggtcctt ttgacctcct tcaacctctc gatctgcact ttctccgcgt tctcccgccg
     6961 gtccgggtca taggggtggg cgaccaggcg ggtggtctgc acctccagct cgctctctcc
     7021 cgtatagcag ttgaccccga taatgaggtc ttgcccctct tccagcctct tctggcgatc
     7081 gtaggcgctg cgggccactt cccgctgcag gtatccgctc tcgatggctt tgaccacccc
     7141 tcccatggac tggatcttgt cgaattccgc ccaggcggcg ttctcgacct ggtcggtcag
     7201 cgattcgaca tagtaagatc ctgccagagg gtccatgacc tcggtaaggc gagcctcgtt
     7261 ctggaggata cgggccgcgt cttcactcag ttgaatcgct tccaagctcc agcccaagcc
     7321 caggggctca tcgaagggag gattgcaatt tggaggtcct ccggacaggg cggcggcgat
     7381 tcccccaacg atggagcggg tcaggttgtt gagggggcgc tggatggagc agttgacccg
     7441 gccacagtga gcggtggagg ccatgcggag aagcatggac cgttcgttct tggccccgaa
     7501 tttttccttc accaccttgg cccacatcct ccgggccgcc cggtggaagg cgatttcttt
     7561 gaagaaatcc atggacccgc cgaaggcatt gaagctgaat cggggggcga agtcgtccac
     7621 atccagtccc gcgttcaccc cttcctggag ataggctgcg ccaatggcca tgctgtaagc
     7681 aaggtcctgc tcccgggtgg cgccggcttc gcggatgtgg aacccgccca tgctggtgat
     7741 gttcacgttg ggaacatgtt tcacaaagaa caccagactg tcccggaaca tgcgcatggc
     7801 atggcggggg ggaaagatgt aagtcgcccg ggcgacgtac tctttcagga tttcattctg
     7861 gggcgtggcc cggagcttgt cccaggagat gccccgctgg tcggccaggg ccaggtacat
     7921 ggccatgatg atgttggccg gggcgttgat ggtgaagttg gaagcagtcc ggtcaaggtc
     7981 agtttccccg gagaaggcct cgaagatgat ctccatgtcc cggagagtgt ccacggccac
     8041 cccaaccttt cccacctctc cggcagccag ggggttgtcc gagtcgtagc cgatctgggt
     8101 gggaaggtcg aaggcgatgt tggggccggc tttctggccc agttttttca tatgcagata
     8161 gtaatcccgc gtatcctccg cagagccgta tcctgaataa cggcctgcgc gggccagccc
     8221 cggagcctgg gcaatggaac ccgaacccct ctcccccgtc cggtagggaa aggtgggaaa
     8281 agtcccggcg gtgaaagggt actgacccgg aaacccaacc ttctccagga atttgaaatc
     8341 ttcccctgta tcgaggggag agtaaaaacg ggtgggggac tctttcagct tgaaccgctg
     8401 gagggccggt ttgagggttt tcccctccca tttttccttt tcctctcgga ccttcttcag
     8461 ggaatcattc tgactcatga cccaaaatct cctttccagt tctgatgaat tcgtaaaaag
     8521 tcggaattcc atagaattcg tcattccggc gaaagccgga atctagttat ttcaagatgt
     8581 tctggacccc ggctttcgcc ggggtgacga ccaaacagac ttcttacgag accatcaatt
     8641 tcccccaggg taaaagaatt ccctcttcat caattgatca ccttggcccc catcttgggg
     8701 aggaattctt tataccatgc agattcagcg gctacatctt tttccatgga cttcccgtct
     8761 ttaaaatcat agggaaggtc caaatgtttc agcagattct gaaattccgc cccctcgact
     8821 attttccggg tagccgtcag gagtttttca tagacgggtt ccggaatgcc tttgggcgcg
     8881 accagaatat accccagggc cggagcatcc gggcagccca gctccttcag cgtggggata
     8941 tcgggatagt cggggtcccg tttgtccgtg ttgtaaacca tgagcatgcg gaagaccccc
     9001 tgtctgacgt actgcatgtg ctgccctgcc ccggcgacaa aatccgtgtg cttgcccatc
     9061 agggccgtgt tggccggagc cccgcccttg tagggcacgt gcttgaggtt caatcccttg
     9121 cactgagaga agagctccac ggctaaatgc tgctgggtgt aggctccgga agacccgtag
     9181 gaaagtccca acttggactt ggcgtaagcg atgaaatcgt cgatggtctt gagcggagaa
     9241 tcattcagga cgcagaggcc gccgatgtag cggctgtact gggcgatcaa agtaaaatcc
     9301 ttgaaggggt cgtaggacac tttgaaaata tgcggccgga cggtaatgac tcctgtggag
     9361 acaactccca gggtataccc gtcgggcttc ctgctggcca ccaagccggc cgccaccgtg
     9421 gctccccccc cggccttgtt ttccaccacc accgggacac cgaggagttt ctccagaccg
     9481 gcgcccagtg cccgggccgt caaatcggtg gtagcccctg cctcgtaccc gcagtagatg
     9541 gtgatgggct tggacggata atcctgggcc gaggtcgggc tgggcaggac cagggttacg
     9601 agtgaaacca ggaaggcgaa aataaagaat cggaaaa
//
LOCUS       DASPRR010000491         8634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1246371, whole genome shotgun sequence.
ACCESSION   DASPRR010000491 DASPRR010000000
VERSION     DASPRR010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246371"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1513)
                     /locus_tag="VEL68_18045"
     CDS             complement(179..1513)
                     /locus_tag="VEL68_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HXZ37931.1"
                     /translation="MMKDQPKITVGIMDRQAEINGCLHGDFSGEGFGPVSGRVSAKVM
                     SGMIVLTDEARLEIARHPSIRLAATGDSAFELFQVTIGTRFHWERKENQTFLGNLILW
                     PRQDGTITAINEIPLEDYLKSVISSEMNATAPVEFLKVHAILSRSWLLAALDRKKKTN
                     DTSRSAHTIPEREGEVIRWYDREDHDLFDVCADDHCQRYQGTQKIVSAQAEEAVRNTL
                     GTVLTHHNEICDARYSKACGGLTEDFDTAWEDRRVPYLTSISDAPVPHPPIGTEEEAA
                     RWILSSPVAYCHTEDEGLLEKILPVFDRETKNFFRWTIEYPREELEEILREKSGFDFG
                     DLQEVVPLRRGPSGRIRRLKIVGSKRSLAVGKELEIRRWLSQSHLYSSAFLVKVKKDP
                     RGKAERFIFHGAGWGHGVGLCQIGAAVMAARGFSAVEILKHYLRGVEIKKIY"
     gene            1810..5982
                     /locus_tag="VEL68_18050"
     CDS             1810..5982
                     /locus_tag="VEL68_18050"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXZ37932.1"
                     /translation="MILGLDFGAETCKAVLLDESLQVMNRWETFSRGNPAGALQTITA
                     SLFGNTGDLQLKIGVTGSGKDAFEFPDKVKILNELISLALGIHHLYPTARSVIEIGAE
                     NSRWLLFGPAPTTEAHPEILDFAMNERCAAGSGAFLRQQAARLKLSLSEFSALAASAQ
                     KGASIAGRCSVFAKSDMIHHQQKGTPLAEIAYGVCLALARNFVATISRGKECFPPICV
                     TGGGALNKGLLRAFRDILGLQENEWVISDWPLFTCALGAALSAAHSGNAIPFENPEDM
                     LRLLKPKKSIPKSKYSSLGALGNQTSTEPSLADREIAQGFLGVDVGSVSTNLALVDGR
                     GRVLAGVYVPTSGRPVEAIREGYGVLKSRCRGELEVSGIGTTGSGRYLAGKLLRADVI
                     HNEITCQMESTVHYFPETDTIFEIGGQDSKYISVRDGKLRDFTMNKVCAAGTGSFLEE
                     QAGPLGVQIEAEFSALAAESQTPYDLGSRCTVFMDTELTQAVGRGVSLPDLTAGLAYS
                     IARNYLEKVVSGRPVGQKIVLQGGVASNASVVRAFSLLLGRTVQVHPFNRISGAIGAA
                     LMAKEKARKDARPSPSHQELEERILQEYSVSSFECPQCSNRCQVNKIQLGSETLFFGD
                     TCERYTSQQTHLPSRTKPLLPDLFKERDDLVKSLIHNPSSPALKIGLPRASFMFEYLP
                     FWVTFFNRLGCAVTLSPDSSGDVLGKGLKELPAETCFPIKLAFGHVKSLLKTDVDWIF
                     FPSLVDLHQNPDESIDLCPYCEHVPFMVRSVLEKQLLTPSILFNSGVGDFVKSMGPIK
                     RILGKDDHSPESAYLEARKAQKDFSRKLRERGEEVVGQFMRKGFQLWAVLGKPYNVHD
                     AFLNLNLGKHLQKLGILPLPIDFIPSNGDQLRDWESRPVWQYNQEIIRASLWCAENPS
                     IFPLILSNFGCGPDALSAKHVEKILDQIPHLLLEFDEHRAEAGLITRLEAFLDEVTST
                     PQDSPKPLLFITQKKGEAKPIDEYRDRPFVLPYFSDHVFAFAGALRGVGISARILPPP
                     DDETLALGEKFSSGKECHAFSFLAGDLAKLARSPREGGEVFFFLGARYTCLLAQFEAV
                     LNYLLQDLGITDLRVFTPSMEPLSKLLRIRGLTLLWRGLVTTDLLIKAACERRPYEIH
                     KGMTDLIHHQNLKDIENGLENGSFGTAMERCAARLESIPIRKEQRPIVGIAGDLYTRQ
                     HPVANHSLYLKLENMGCEVWPPPFLIDDVDFSLKRSFGASLAKLRLHEAAATGLLILR
                     KEVERWKVRKNFGEALPRLGEPSSKDILEFVAPYIGWDNNQTLLLNIAKMVDFARRGA
                     DGVVNAICFNCMLGTVSEALAVQVRRDYKNIPLPTLIYGTTELSSEKSKLEAFVYQVH
                     QFAKKKTVGREQ"
     gene            complement(6102..6740)
                     /locus_tag="VEL68_18055"
     CDS             complement(6102..6740)
                     /locus_tag="VEL68_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37933.1"
                     /translation="MKTRKLYLILLLLVFAVGCAIPVREAVKVDMKAPVGKIEGNQFT
                     GIRYPFNVAAPPGWQVTTQYPKFMIDLGYDKEGLEASAVFLFNPATQSNLQIDFEAAD
                     RYSTFSQKSIEALTTAATGSFQEELEKDLGRGAKAEIGPTEPVTLKGVPYAAKKYATY
                     TAKGVKREQGWIYAFAEPYQIFILYLILDKEGKNDHEAFKAILDSFEYIPKK"
     gene            complement(6986..7444)
                     /locus_tag="VEL68_18060"
     CDS             complement(6986..7444)
                     /locus_tag="VEL68_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXZ37934.1"
                     /translation="MSKKVIDLLNAARSRELGAITQYMAQHYELEDQDYGKLAKKLKE
                     IGIQEMKHAEALAERILFLKGEPTSKPDGVAKKGQSIADMLKTDMALEIAAIKMYNES
                     AVICAAEKDQISKQLFEKLLGEEEGHFNDFENVKDHLEKMGDAYLATLAG"
     gene            complement(7560..8528)
                     /locus_tag="VEL68_18065"
     CDS             complement(7560..8528)
                     /locus_tag="VEL68_18065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ37935.1"
                     /translation="MKRLFYGLVLGVFLLCPVLGMAAEKFPAKAIELDVPFAAGGSTD
                     VLARLVSKFAPKYFDKPLIVVNKPGGGGITGTEGVVRSKPDGYTIYVGYGSGHDLVTP
                     HFQKMPYDTFNDLVSVCRLSIHSIVMIIRGDAPYKTMKEFVEWGKKKDQVTASVSTKA
                     GSVDITFQAVGKATGLKMVTVPFRGGAESVTAIVGGQTDCGGNHPSEIISHIKAKRLI
                     PIAVALENRDPAIPDVPTFKELGYNVVAVGSVKGVAAPKGTSPEVVRYLEERFKKVCD
                     DPEFIKSMKDIGQPVMYQGSQEFAKYMKDGFAQYGQMIKEFNIKLE"
BASE COUNT         1933 a         2433 c         2230 g         2038 t
ORIGIN      
        1 gccggcgccc aggatgaagg cggtcttgaa tcgccgtcct ttcaaagaca cctcgcgtct
       61 ttttcaattc tcaaatccgg gcaaatttgc acgagagccc accaaacccg ttctgggtca
      121 aaaccctatt tcccccttgc cccccaaaac atccgctccc accggctatt tgcctgccct
      181 aataaatctt ctttatttcc actccccgga ggtaatgctt caggatttcc accgccgaaa
      241 atccccgggc agccatgacc gcggcgccga tctgacagag ccccaccccg tgtccccagc
      301 ccgccccgtg aaagataaat cgctcggctt tcccgcgggg gtcctttttc actttcacga
      361 ggaaggcact gctgtaaagg tgactctggg aaagccaccg acggatttcc agttcctttc
      421 ccacggccag gcttctcttc gaacccacga ttttcaacct ccggatcctc cccgaagggc
      481 cgcggcgaag gggtacgacc tcctgcagat cgccgaaatc aaaacctgat ttttcccgca
      541 ggatctcttc gagttcttcc cggggatatt cgatcgtcca ccggaaaaaa tttttggtct
      601 cgcggtcaaa gacgggaagg attttttcga gaagaccttc atcttctgta tggcagtacg
      661 ccaccgggga ggagagaatc cagcgggccg cttcctcctc tgtcccgatc gggggatgcg
      721 gaaccggtgc atccgaaatg cttgtcagat aaggaacgcg cctatcctcc caggccgtgt
      781 cgaagtcctc cgtcagtcct ccgcaggcct tagaatatcg ggcatcgcag atttcattat
      841 ggtgagtgag taccgtcccg agggtattcc gcaccgcctc ttcggcctgt gcagatacga
      901 tcttctgggt cccctggtaa cgctggcagt ggtcatcggc gcagacatcg aaaagatcgt
      961 ggtcctcccg gtcataccag cggatgactt ccccttccct ttcggggatc gtgtgcgccg
     1021 agcgggatgt atcgtttgtt ttcttcttcc ggtcgagggc ggccagaagc cagcttcggg
     1081 aaaggatggc gtggactttc agaaattcaa ccggagctgt ggcgttcatc tccgaggaaa
     1141 tgacactttt caggtaatcc tctaaaggaa tttcattgat ggccgtaatc gttccatctt
     1201 ggcgcggcca aagaatcaga ttcccaagaa aggtctgatt ttctttcctc tcccaatgga
     1261 accgggttcc gatggtcacc tgaaacagtt caaaggcgga gtctcccgtg gctgcgaggc
     1321 ggatcgatgg atggcgagcg atttcaaggc gggcttcatc ggtaagcaca atcatccccg
     1381 acattacctt tgcggaaacc cggccggaca cagggccgaa cccttccccg ctgaaatctc
     1441 catgcagaca gccgttaatt tcggcctgcc ggtccatgat ccccaccgtg attttcggtt
     1501 ggtctttcat catgtggtta aacccttgaa aaataggtcg gtccaagtta aattattccc
     1561 cactttacca caaaaacaga cgattttccc ctcgcccatt cccttcttct gatcgtggag
     1621 gataatggag caggctctga aaggggcgag acagagacct aaatccgatg atttttgttt
     1681 gagtaggaga cccggacccg tataatactc ttatgagtaa acaaatgaac aggcgtctct
     1741 gacgacaggg ctcagaaaat tggttagcat cattgaggtt aactccttgc agaaggggaa
     1801 aaaaacgaga tgatcctggg actcgacttt ggggctgaga cctgcaaggc cgttcttttg
     1861 gatgagtctc ttcaggttat gaacaggtgg gaaaccttca gccgcggcaa tccggccggc
     1921 gctctgcaaa cgattaccgc cagtcttttc gggaacaccg gggacctcca gctcaagatc
     1981 ggcgtgacag gctcaggaaa agatgcgttt gaattccctg acaaagtcaa gattctcaac
     2041 gagcttatct ctctcgccct cggcatccac catttgtacc caaccgcccg ttcggtaatc
     2101 gagatcgggg ccgagaactc ccgttggcta ctctttggcc cggctcctac gacagaagca
     2161 cacccggaaa tcctggactt tgccatgaac gaaagatgcg ccgccggatc aggggccttc
     2221 ctgcgccaac aggctgcaag gctaaaactc agcctttccg agttttcggc gcttgccgcg
     2281 tcggcccaga aaggggcatc gattgccggc cgctgctcgg tctttgccaa gtccgatatg
     2341 atccatcatc agcagaaagg aacccctctc gccgaaatcg cctacggagt ctgtctcgct
     2401 cttgccagga actttgtggc tacgatatcc cgcggcaaag agtgttttcc tccgatctgt
     2461 gtgactggag gcggcgccct gaacaaaggg cttctaaggg catttcgaga cattctcggg
     2521 ctccaagaaa acgagtgggt catctccgat tggccgcttt tcacctgcgc cttgggggcg
     2581 gccctgagtg ccgctcactc aggaaatgcc atcccctttg agaaccccga ggatatgctg
     2641 cgcctcttga agccaaagaa atccattccc aaaagcaaat attcgagttt gggcgcattg
     2701 ggcaaccaga ccagcacgga gccatccctc gccgataggg agatcgccca aggttttttg
     2761 ggagtcgacg tgggctcagt cagcaccaac ctagccctcg tcgacggacg aggacgagtt
     2821 ctagccgggg tctacgtccc gaccagcggg aggccggtcg aagcgatccg tgagggatac
     2881 ggggtcttga agtcaagatg ccgaggggaa ttggaggttt cggggatcgg aacgaccggg
     2941 agcggacgct acttggcagg aaagctcctg cgggcggatg tcatccacaa tgaaatcacc
     3001 tgccagatgg aaagcaccgt ccattatttt ccggagacag atacgatttt cgaaatcgga
     3061 ggccaggatt ccaaatacat cagcgtcagg gatggaaagc tccgtgattt caccatgaac
     3121 aaggtctgcg cggccgggac tgggagcttt ctcgaggaac aggcagggcc cttgggggtt
     3181 cagatcgagg cggaattctc ggccttggcg gccgaatccc aaactcccta tgacctggga
     3241 tcgaggtgca cggtcttcat ggacaccgag ctcacccagg ccgtgggccg gggtgtctcc
     3301 cttcccgacc tgacggccgg cctagcctat tccatcgctc ggaactacct ggagaaagtc
     3361 gtctccggcc gcccggttgg ccagaaaatc gtcttgcagg ggggagttgc ttccaacgcc
     3421 tcggtggtta gggcgttttc gcttctcctg gggcgtaccg tacaggttca tccattcaac
     3481 cggatttccg gagcgatcgg ggcggcgctg atggccaaag aaaaagcccg gaaggatgcc
     3541 aggccttcgc cgagtcatca ggagctggag gagagaatcc tgcaggaata ctctgtttcc
     3601 agcttcgaat gtccccaatg ttccaaccgt tgccaagtca acaaaattca gctgggcagc
     3661 gagacgctct tctttggcga tacctgcgag cgttatacct cacaacagac ccatctcccg
     3721 tcccggacca aaccgctgct tcccgacctt tttaaagaac gggatgacct ggtcaaatcg
     3781 ctgatccaca atccttcttc ccccgccctg aagatcggcc ttcccagggc ctcattcatg
     3841 ttcgagtatc taccgttctg ggtgaccttt tttaatagat tgggatgcgc tgtcaccctc
     3901 tcccccgatt ccagcgggga cgtgctggga aaagggctca aggagcttcc ggcggaaaca
     3961 tgctttccga tcaagttggc cttcggtcac gtcaaatctc tcttaaaaac ggatgtggac
     4021 tggatttttt tcccctcgct ggtcgatctg caccagaatc cggatgaatc gatcgatctc
     4081 tgtccctact gcgaacatgt tcctttcatg gtccgatccg tcctggaaaa acagctcttg
     4141 actccatcca tcctttttaa ttccggggtc ggcgattttg tgaaatcaat ggggcccatt
     4201 aagagaatcc tgggcaaaga tgaccattcg cccgaatccg cttaccttga agcccggaaa
     4261 gctcaaaagg acttcagccg caagctgagg gaacgggggg aggaggtcgt cggccaattt
     4321 atgagaaaag gcttccaact ctgggctgtt ctcgggaaac cctacaatgt tcatgatgcc
     4381 ttcctcaatt tgaacctcgg caaacatctt cagaagctgg gaattcttcc cctgccgatc
     4441 gattttattc cgtccaatgg ggaccaactc cgcgattggg aatcccgccc ggtctggcaa
     4501 tacaatcagg agatcatccg ggcgtctctt tggtgcgccg agaatccgtc tatctttccg
     4561 ctcatccttt caaatttcgg gtgtgggccg gatgccctat cggctaagca cgtcgaaaaa
     4621 atcctggatc agatccccca ccttctactc gagtttgacg aacacagggc agaagcagga
     4681 ctcatcaccc ggctcgaggc cttcctggac gaggtgacat ctacacccca agactccccc
     4741 aagcccttgc tttttatcac ccagaaaaaa ggagaagcca agccgatcga tgaataccga
     4801 gatcgcccct tcgtccttcc ctatttttca gaccacgtct ttgcctttgc aggagccctc
     4861 aggggcgtgg gcatctccgc tcgcatcctg cccccgcccg acgatgagac gctcgctttg
     4921 ggagagaaat tcagctcggg aaaggagtgc catgcctttt ccttcctggc cggcgacctc
     4981 gccaaattag cccggtctcc acgggagggt ggtgaggtct tttttttctt gggcgcccgg
     5041 tacacctgcc ttctggccca gttcgaagcc gttctgaact atctcctcca ggatttggga
     5101 atcacggacc tgagggtctt cacgccctcg atggaacctc tttccaagtt attgagaatc
     5161 cgcgggttga ccctgctgtg gagaggactt gttaccacag accttttaat caaggcggcc
     5221 tgcgaacgaa ggccatacga aattcacaag ggcatgaccg acttgattca tcatcagaac
     5281 ctcaaggata ttgagaacgg gctggagaat ggctcttttg ggacggcgat ggagaggtgc
     5341 gccgctcgcc tggaatccat tccgatccgt aaagagcaac gtcccatcgt cggcatcgcc
     5401 ggggaccttt acactcgcca gcatccagtg gccaatcaca gcctctatct gaaactggaa
     5461 aacatgggct gcgaagtctg gcccccgccg tttcttatcg acgacgttga tttcagcctc
     5521 aagcggagct tcggcgcaag tctcgccaaa ttgaggcttc acgaagccgc cgccaccggt
     5581 ctcctcatct tgagaaagga agtcgagcgt tggaaggtga gaaaaaactt cggggaggct
     5641 ctacctcgcc tgggagagcc ctcttccaag gacatcctag aattcgtcgc tccttatatc
     5701 gggtgggaca acaatcagac tcttctcctt aatatcgcca agatggttga ctttgccaga
     5761 cggggtgcag acggggttgt caacgcaatc tgcttcaact gcatgctggg cacggtctcg
     5821 gaggctttgg ccgtacaggt ccgcagggat tataagaata ttccattgcc cactttgatt
     5881 tacggcacga ccgagctttc ttctgaaaaa tcaaagctcg aggccttcgt ctatcaggtc
     5941 caccagtttg ctaagaaaaa gaccgtaggc cgtgagcagt gaatttatcc ataagctgct
     6001 agaaaaacaa ggtcaggaaa caaaggggca aatttccctg aatctattcc tccttacgag
     6061 atgcttggaa gccgtcaagc gataaaactc cgccattctt gctatttctt tggaatatac
     6121 tcgaacgaat ccaggattgc cttgaaggcc tcatggtcgt tcttcccttc cttgtctaaa
     6181 atcaaataaa ggataaaaat ctggtagggc tcggcgaagg catagatcca accctgttcc
     6241 cgcttgaccc ccttggccgt gtaggtggcg tatttctttg cggcgtaggg aacccccttt
     6301 agggtgacgg gttcggtggg gccgatctcc gccttcgctc ccctgcccaa atctttttcc
     6361 aattcctcct ggaaacttcc cgtggccgcg gtggtcagcg cttcgatcga tttctggcta
     6421 aaagtggaat agcggtcggc ggcctcaaaa tcgatctgca ggtttgattg ggtcgcgggg
     6481 ttgaaaagaa agaccgcgga cgcctccaag ccttccttgt cataccccaa atcgatcatg
     6541 aatttagggt attgggtggt gacctgccag ccgggaggag ctgcaacgtt gaaggggtac
     6601 cggattccgg taaattgatt cccttcgatc ttccccactg gggctttcat gtctaccttg
     6661 accgcttccc ggaccgggat ggcgcagccc acggcgaata ctagaaggag aagaatcagg
     6721 tacaatttcc tcgttttcat attaccctcc gaaaagtctg atatacgaag gccggaagcc
     6781 atgagcaatg tatcaagacc ttatgaatat tgtaagccca aggttccaga acctgtcaag
     6841 gaccctactg agctaataaa gtggatttcc ccgaagggaa gatggaagaa gaaggtgaaa
     6901 aaaggggtat atcccgcaac ttttccctat ggaatatacc ccgccgaaat caataaagat
     6961 tgtttacccg taaaaggctg gggaatcagc cggccagggt ggccaggtaa gcgtcgccca
     7021 tcttttccag atggtccttc acattctcga aatcattgaa atgcccttct tcctctccga
     7081 gaagtttttc gaagagctgc ttggagattt ggtctttctc cgcggcgcag atcaccgcgg
     7141 actcattgta catcttgatg gccgcgatct ccagagccat atcggtcttc agcatatcgg
     7201 cgatggactg cccctttttg gctaccccgt caggcttgct ggtgggttca cccttgagaa
     7261 acaagattct ttcagccagc gcctcggcgt gcttcatctc ctggatgccg atctctttca
     7321 acttcttggc caatttaccg taatcctgat cctccagttc ataatgctgg gccatatact
     7381 gggtgatagc ccccagttct ctggaccggg cggcattcaa aagatcaatc actttcttgc
     7441 tcataatttc ccccctttga attttggtta taataatcct ccatttttta gaataaatgt
     7501 accctcctcg cttgtaaagg agaaaaacaa cccttgccgg gtggccggcc gcaacgggcc
     7561 tattccagct tgatgttgaa ttctttgatc atctgtccgt actgggcgaa cccgtccttc
     7621 atgtacttgg caaattcctg ggacccctgg tacatgaccg gttgtccaat gtccttcatc
     7681 gatttgatga actccggatc gtcacacacc tttttgaagc gctcttccag gtaacggacc
     7741 acttcggggg aagttccctt gggagcggcc acgcccttca ccgatccgac cgccaccacg
     7801 ttgtagccca gctccttaaa ggtaggaaca tcgggaatcg ccgggtcgcg gttctccaag
     7861 gccacggcga taggaatcag ccgtttggcc ttgatgtggc tgatgatctc cgagggatgg
     7921 ttgcctccgc agtcggtctg cccgcccacg atggccgtca ccgattccgc cccgccgcgg
     7981 aaggggacgg ttaccatttt taagcccgtg gcttttccca cggcctggaa ggtgatatcc
     8041 acagagccgg ccttggtgga gaccgaagcc gtaacctgat ccttcttctt tccccactcg
     8101 acgaactctt tcatggtttt gtaaggagca tcgccacgga tgatcatgac aatggaatgg
     8161 atcgacaagc ggcagacgga gaccaggtca ttgaaggtgt cgtacggcat cttctggaag
     8221 tggggggtga ccaggtcgtg tccggaacca tatcccacat aaatggtata gccatcgggc
     8281 ttggaccgga cgaccccttc cgttcccgtg atccctccgc caccgggttt gtttacgacg
     8341 atcaggggtt tatcgaagta tttgggagcg aatttcgata ccagacgggc taaaacatct
     8401 gtggaccctc cggctgcaaa ggggacgtcc aattcgatag ctttggcagg aaatttttcc
     8461 gccgccatcc caaggaccgg gcagagcagg aaaacaccga gaaccaatcc atagaataat
     8521 cttttcatct ttggtttcct ccatggtgag agattttttt tcaggtgtgg ttgctagtct
     8581 ttttctttcg caagacgata aatacttgaa gcacgataaa aagaatcaaa agcc
//
LOCUS       DASPRR010000492         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1569364, whole genome shotgun sequence.
ACCESSION   DASPRR010000492 DASPRR010000000
VERSION     DASPRR010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569364"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            991..2895
                     /locus_tag="VEL68_18070"
     CDS             991..2895
                     /locus_tag="VEL68_18070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ37936.1"
                     /translation="MRIGTKLLLGFIPLAILTAMVAEVLYVNSQRISESSHRVREAYQ
                     NYSNILDMRRHEKNFAFYREKFYLQRIQEASSRAAESLSQLRVLGDENESLFKFTQAQ
                     AVLRDYQRVIGLLLSAEGRPEADQKIAELEALGHQLEKMAEQAIGLSWLPIQVATRKA
                     QTYAWVFAVQAGLIGVVLAFYFSQLLVKPIRQLLRGTQKVAEGDFSQGVSLKSRDEIG
                     ALAESFNKMVASLEQGRTQLQQSAQELRKTKDTLENIVQSSVDAIVATDPKGRITFAN
                     RSMQELILGQAGQEEKLLGVPMSQLYSGGLGEARKIMSVLRKRGQLMNYETTMVSNGR
                     VIPILTSASLLKEERGAVIGTLGVIKDLTEKKKLEEDLKKAQAELVQTEKLVAIGRLA
                     SGVAHEMNDPLTSILTFGSLLREDTPEGEPIRESLDIILKEANRAKRIVSDLLSFSRE
                     SKSSMEWVDLNDVLNMSLLLLEKQGVMEGIEVRMDLAKELPLVRADSGQMHQVFTNLL
                     LNSVQAMNPDPSAQEISEFPHGGRKLFLRTSFVETPEVDFSVSPSLAGPFIRAVFQDT
                     GPGIPPENLSRVFDPFFTTKGTGEGTGLGLYIVSGILKNYGAQYRLESTVGRGTTFTI
                     DFPLSTAQKI"
     gene            2897..>4119
                     /locus_tag="VEL68_18075"
     CDS             2897..>4119
                     /locus_tag="VEL68_18075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ37937.1"
                     /translation="MEKATQILIVDDQVNTCKSLQAILKKSGYPSDYTLNGEEALKKA
                     REKHYDIVISDIRMPGMDGMQLLEELKKTQPSLVVIMITGYATIKSAVEAIQKGAYDY
                     LPKPFTPDEVRVIIERAAEHSRLEAENILLREHFQARRPFENIIGNSPALQRVFEMVQ
                     KVADTDSNILLTGETGTGKELVAKAIHDRSRRREKLFLPLNCGSIAEGVLESELFGHM
                     KGAFTGAVATRKGLIEAADGGTLFLDEISETASSFQVKVLRVIQEGEFIRVGDTHPLR
                     VNIRIIAATNRDLEKAVERREFRQDLYYRLKGIAIHLPPLRERLDDIPSLAYHFLAKY
                     RGDRKAERIDPQALKALQAYKWPGNIRELEHIIEYALSMAEGETIKITDLPEEFINRI
                     ADRAAAQESWANAPF"
BASE COUNT          930 a         1175 c         1125 g          889 t
ORIGIN      
        1 tcgtgcttgt tggggttcag ggtggaggaa tttggatcat caggctcggc gggtgcttat
       61 ctttccatga aacccctccc tatgttccgc agggagaggc ctgagaggag attcctccaa
      121 gcagaaaaga gaaccaaagc atgtttccgt ttcaatcccc ccccaggaca ggcccgatcc
      181 aatggaagaa ccaccgcctc aggatgatga tccaaatttc tccgccctga accccatgag
      241 gtcccccctc ctttccagag atcttagttt cgaaagactc ttttttaaga actgggggtg
      301 ggcaagtcct gcggcgcttg cctccttcgc tttttttctt ttctgtgaaa aagaaaggtc
      361 gtggcccgga agcaaaggca ttgtgcggga aaagcatcgg cccttccatt tcggatttcg
      421 atttgcggat ttcagatttt gggcttttct ttttcaatcc gcattccgca atcaacaatt
      481 cgtaatttcg aaggcccgcc cggtccaagg aatgaaaagt atctcatgat ttttgcgcgc
      541 aatggccttg tttccggaac aggcaattcc tgaaattcaa cagagcttac gtctatttgc
      601 cgaaaagtga cggggccagt ccaacggcct tgattccgtt ttcatgcctg ttggcataag
      661 accccggatt tcgagcgcat tggatggcga gtgcgagtac cgccgggttt cccctcatta
      721 aggttttccc ctcctctttc cgcagacttg ccggagaatg ggtttccgaa cggcggcctc
      781 gccgagccgc agcccggcag ctctttttcc tggttctctt ttcacttgct tcttttttca
      841 atttaaccct ataatttcaa tagaatatga gtcgttcgga aatcatggaa acggtagcgt
      901 tccgagatat gttcttaacc caggggtgcg ccgagacgca cgcatccgag acttttttcc
      961 cctttagccc tcacttgatt tactagaaac atgcggatcg ggaccaaact ccttctcggt
     1021 tttatccccc tggccattct cacggccatg gtcgccgagg tattatacgt caactcccaa
     1081 cgaatcagcg agagctctca ccgggtgagg gaggcctacc agaattacag taacattctg
     1141 gacatgcgcc ggcacgaaaa gaacttcgct ttttaccggg aaaaattcta cctccagcgc
     1201 atccaggaag cttcctctcg cgcagctgag tctctttccc aactgcgggt cctgggggat
     1261 gagaatgaat ccctctttaa attcacccag gcccaagcag tcctgaggga ctatcagcgg
     1321 gtcattggtc tcctcctttc cgcggagggc cgccccgagg ctgaccaaaa gatcgctgaa
     1381 ctggaagcct tgggccacca gttggagaaa atggccgaac aggccatagg cctctcctgg
     1441 ctgccgatcc aggtggccac ccggaaggcc cagacctatg cttgggtctt tgccgttcaa
     1501 gccgggctga tcggcgtggt tctggctttc tacttcagcc agctcctggt gaagcccatt
     1561 cgacagctcc tgcgggggac ccagaaggtg gccgaaggag atttttccca gggggtctcc
     1621 ctgaagtcca gggacgaaat cggggcgctc gccgaatcct tcaacaagat ggtggcctcc
     1681 ctggagcagg gccgaaccca gttgcagcag tcggcccagg aactgcgcaa aaccaaggat
     1741 accctcgaga acattgtcca gagctcggtc gatgccatcg tggccaccga tcccaagggc
     1801 cggatcacct ttgccaaccg gtccatgcag gagcttattc tgggccaggc cgggcaggaa
     1861 gagaagcttc tcggggtgcc catgagccag ttgtattcgg gggggctggg ggaggctcgt
     1921 aaaatcatgt cggttctccg gaagcggggc cagttgatga attatgaaac cacgatggtt
     1981 agcaacgggc gggtgatccc catcctgacc tcggcttccc tgctcaagga ggagcgcgga
     2041 gcggtcatcg gaaccctggg ggtgatcaag gacttgaccg aaaagaagaa actggaggag
     2101 gacttgaaga aggcccaggc cgagttggtc cagaccgaga aactggtggc catcggccgg
     2161 ctggcctccg gggtggccca cgagatgaac gaccctctca cctccatcct gaccttcggc
     2221 agtctcctgc gggaagacac cccggagggg gaaccgattc gggaaagttt ggacattatc
     2281 ctcaaagagg cgaaccgagc caagagaatc gtctctgacc ttctctcctt ctctcgggaa
     2341 tccaaatcct ctatggagtg ggtggacctc aatgatgtgt tgaacatgag tcttctcctc
     2401 ctggaaaaac agggggtcat ggagggaatt gaagtccgga tggacctggc caaggaactc
     2461 cctctggtgc gtgccgattc cgggcagatg catcaggttt ttaccaatct tcttcttaat
     2521 tcggttcagg ctatgaaccc cgatccttca gctcaagaaa tatccgaatt cccccacggg
     2581 ggaaggaaac tctttctccg aactagtttt gtcgaaactc ccgaggtaga tttttccgtt
     2641 tccccttccc tggccgggcc tttcatccgg gcggtctttc aggacaccgg gcccgggatc
     2701 ccacccgaaa acttgagccg ggtcttcgat ccctttttta ccactaaggg caccggggaa
     2761 ggcaccggct tggggcttta tattgtctcg ggaattctga aaaactacgg agcccagtat
     2821 cgcctggaga gcacggtggg tcggggaaca acttttacca tcgatttccc tttgtccact
     2881 gcgcagaaaa tctgatatgg aaaaagcgac ccaaattctt atcgtcgacg accaggtcaa
     2941 cacctgcaaa tcccttcagg ccatactcaa aaagagcgga tacccatcgg actataccct
     3001 gaatggggag gaagctctga agaaggctcg agaaaaacac tacgatatcg tcatcagcga
     3061 tattcgcatg cccggcatgg acgggatgca gctcctggag gaattgaaga agacccagcc
     3121 ctccctggtg gtcatcatga tcaccggata tgccaccatc aagagcgccg tggaagccat
     3181 ccagaagggg gcctatgatt acctgcccaa gcccttcacc cccgacgaag tccgggtgat
     3241 catcgagcgg gccgcggagc acagccggct ggaggccgag aatattctct tacgcgagca
     3301 tttccaggct cggcgcccct ttgagaacat catcggcaac agtccggccc tgcagcgggt
     3361 cttcgagatg gtccagaaag tagccgacac cgacagcaac atcctcctca cgggagaaac
     3421 gggaacggga aaagagctgg tggccaaggc catccacgac cggagccgga gacgggagaa
     3481 gctgttcctt cctctcaact gcggctccat cgccgaaggg gtattggaaa gcgaactctt
     3541 cggccatatg aaaggcgcct ttaccggggc cgtcgccact cgaaaaggcc tgattgaggc
     3601 cgccgatggg gggacgctct ttctcgacga gatctccgaa accgcctcct ccttccaggt
     3661 aaaggttctc cgggtcattc aggaggggga gttcatacgc gtgggggaca cgcaccctct
     3721 ccgggtaaac atccgcatca tcgccgccac caatcgtgac ctggaaaagg cggtggagag
     3781 gcgggagttc cgccaggacc tctactaccg gctcaaaggg attgccattc acctgcctcc
     3841 attgcgggaa cgcctggacg acattccctc cctggcctat catttcttgg ccaagtaccg
     3901 cggggaccgg aaagcggagc gcattgatcc ccaagccctg aaagcccttc aggcttataa
     3961 atggccggga aacatacgcg agctggaaca tatcatcgaa tacgccctct ccatggcgga
     4021 aggagagacg atcaagatca ccgacctccc ggaagagttc atcaatcgga tagcggaccg
     4081 ggcggcagcc caggagagtt gggccaatgc ccctttcag
//
LOCUS       DASPRR010000493         6487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_773343, whole genome shotgun sequence.
ACCESSION   DASPRR010000493 DASPRR010000000
VERSION     DASPRR010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773343"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..416
                     /locus_tag="VEL68_18080"
     CDS             126..416
                     /locus_tag="VEL68_18080"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXZ37938.1"
                     /translation="MRFFGPLEKKYLVFLALLILGVLLFAVFGSRGLMQIYQLREERD
                     RIQMSNTRLEDENKKLSEQIQRLRTDKDEIERVAREEMKLVGKGEIVYQFEK"
     gene            884..1084
                     /locus_tag="VEL68_18085"
     CDS             884..1084
                     /locus_tag="VEL68_18085"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXZ37939.1"
                     /translation="MNSLRKYRERLLMSKAELARKAGLSALTIDRIEKGKSCRLETKR
                     KIILALGLQLADRGKIFTEIKT"
     gene            1129..2358
                     /locus_tag="VEL68_18090"
     CDS             1129..2358
                     /locus_tag="VEL68_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37940.1"
                     /translation="MRTKFFPDVGIRAVGRKGTPKPILGVAALLEWVLLPIFLFSFLL
                     GGGATAAEKPALPPSPTEQEVATEKFLSQLEATLAQKFFDFRIRPVIRVAILDFTDGA
                     GNVVKGGVDWADRIARRLYPQPQFDLVSRDRVSQYLSWNSLGTVGKLDAAGLLLLQRR
                     INTMDPGNGIHVLLTGEITKGTGRTMQVQAYLTNFEQRLGERELEKNLIDLLTVSGEV
                     PLPTEQALQEVSEVVVRGEKHAFTEGRLLILASTRGYPLVETEYLNQFSKDQPFAWDQ
                     IPYALVVGKEEYAMPKQVQIGAGKVILHPLQVPMNSPKRLEHSFLHGKCATNEVYFDE
                     MIPAMKYQLTGSFIDFKNGQTFSETAEVEVYPGATTVVILSFFVPSEKERIRNRTFPG
                     ISIYQLNGKGLEILPRG"
     gene            2365..3420
                     /gene="pilM"
                     /locus_tag="VEL68_18095"
     CDS             2365..3420
                     /gene="pilM"
                     /locus_tag="VEL68_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXZ37941.1"
                     /translation="MGLFSKNELLGLDIGSNSIKAVELDWRKKSPRLKIFGMIHLPPE
                     AIVDGAFMDSASIVDSIRSLVEGLKVKTKNVAVSISGHSVYIKTINVPNMSKEQLEES
                     IKWEAEQQIPFDIEDVNIDFQILDAQTTPDQMPVLLVAAKKDMINDYTAVIEEAGLAP
                     TIVDVDSFAIENAFNLTNDSPPDEVLALVNIGASVMNINILQGGTSAFTRDISIGGRQ
                     ITEEIQKRLKMTYEEAEALKVKERDAGAQSAEVDKIIQGTAEQLATEVRRSLEFFAAS
                     SSFGEIKKIHLSGGCAKIPMLPALIEERIGTPVEVFNPFAKIEYNTDEFSPEYIREVG
                     PLAAVAVGLALRREDLK"
     gene            3417..3989
                     /locus_tag="VEL68_18100"
     CDS             3417..3989
                     /locus_tag="VEL68_18100"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXZ37942.1"
                     /translation="MIRINLLPVREKKKKESTRKMVSLLILSLALVAVVITFFHFSLY
                     SQINEVQRQITAYNDEIKRLKIDTKDVDKFKAEKEDLQRRINIIYTLQRAKTGPVRVL
                     DDLVTSLPGRLWLVALREKSGKMEIKGIAMDNQDIAKFMTNLEKSGVIRNVELVVSQQ
                     LERKELKLMEFTLTCQVDYANLPVGIVKGG"
     gene            3993..4634
                     /locus_tag="VEL68_18105"
     CDS             3993..4634
                     /locus_tag="VEL68_18105"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXZ37943.1"
                     /translation="MAITLESIVKLPTSRKILILAGIVAAMIGLYIYLIYWPNLDLLK
                     KKKAEMGSLENQVRELRIVAANMKRFQAEAAKLREELQVAITQLPTSKEIPTLLSNVS
                     QLGKDAGLEFLLFRPTAEVNREFYAEIPVEIRVKGTYHNVALFFDRVGRLPRIVNISE
                     VSMENAKETLGRWEISTACTATTFKFIEKSPAELAKEKEKKTGESKGTPPKKP"
     gene            4645..5217
                     /locus_tag="VEL68_18110"
     CDS             4645..5217
                     /locus_tag="VEL68_18110"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HXZ37944.1"
                     /translation="MMGKTSILLLLGVFLVSVGCGGGEAPKPTKVPIPPTAPKPVAKV
                     SGKEVPQPAAEVPVEPPLATSYAYNPQGKANPFQPLVVEKPSGPPPKKKVEQASKREK
                     EEPGTPLERLDLPALKLVAVVWNIPHPRAMVEDSTGKGYILSLGTRIGKNHGQVTQIT
                     SAGVVITEKVEGADGKLRTLETPLRLYADQ"
     gene            5228..>6487
                     /locus_tag="VEL68_18115"
     CDS             5228..>6487
                     /locus_tag="VEL68_18115"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HXZ37945.1"
                     /translation="MEGNRWKIIFCLISLGAWVFLSLASPSFAQGPPGPAEGYAIQRI
                     EVTKTESGSRVSVEGSKSFEYTVFRLANPLRVVIDLPQAQLGKLAGPMEVRDGTISVI
                     QNRQVGDPQKRGARIEIGLEQLVEYDVNPEGKFLYINFGRPAIPLPQVAKEAVKKEEP
                     AKEFPPPPAKPLMKARSLRGVDISAKADWVEVDLRGDGLIPEYNSFQLARPTRLAVDI
                     PKMTNASGKANINVGNRLLKEIRIGQHADKIRVVFTFPDAKIPQYQLAKEGAELKLIL
                     GEVKKEAPMTEKTPPAEPPKTEQVAEAPSKAKEDVAPAAPVPEKPAIKEEKAPPPESP
                     KPVMVAEALPKAKEEAAPTPPAPEKPSIKEEKALNAETKPPEPERAPVGGYRGSKISL
                     DFKDADIHNILRLIAEVSNLNIITSEDV"
BASE COUNT         1677 a         1676 c         1801 g         1333 t
ORIGIN      
        1 aagactttgc cctcccctca gaagcaaaca tggaagaagg aatcggggtt tgaaaaatcc
       61 actttacttt tgaaatggag aaggcatatg atttcaaaag gcttgaaagg agatgccttc
      121 actcggtgcg attcttcggg ccgcttgaga agaaatacct ggtctttctt gcccttttga
      181 ttttgggggt gctcctcttt gcggtcttcg ggagccgggg cctgatgcag atttatcaac
      241 tgcgggaaga gagggaccga atccagatga gcaacacccg gctcgaggat gaaaataaaa
      301 aattgagcga gcaaatccaa cggctccgaa cggataagga cgaaattgaa agggtcgccc
      361 gggaggagat gaagcttgtc ggcaagggag agattgttta tcagttcgag aagtgagaga
      421 caagccgcca aagcggaacg ctttttttct gaaaccggaa cgcaaatcaa ctgatttccc
      481 ttttcatcct tctcaattgt tatctttgct agccacgcct tgcgtcatcc atcctcacgt
      541 ttttgtgtca aaattgactc ggggggacaa gggtttttgg cacaaaaaag tacagatatg
      601 aacttccatg gacacccagg aaatatgaaa tagcgcgatc ttcaacaagc agcgatcaga
      661 gtcggggtcc ggccttcaaa aattgaattt ttccctcaac cataaaaagc tataaaaatt
      721 agatatttgg tcagtaataa agatatataa ttattaataa ttttattaaa attatttaaa
      781 atttaaaaat attgacaaat gtgatggctt atggaattat ttttgcactt aggttttctg
      841 tctcttgtgt cgaagagggt tttagacccc caaaagatcg aagatgaact ccttacggaa
      901 ataccgtgaa agactattga tgagcaaggc cgaactggcc cgtaaggcag gcctttcggc
      961 gctgaccata gaccggattg aaaaagggaa gagctgccgc ctggagacca aacggaagat
     1021 tattttggcc ttgggattgc agctggcgga ccgggggaag atcttcactg aaatcaaaac
     1081 ataatcccgg ccatttcatt taccgattca aagattaggg gaagacctat gagaacgaag
     1141 ttctttccag acgtgggaat cagggctgtc gggagaaagg gaacgccgaa gcccatcctc
     1201 ggggtagcag cgctcctgga gtgggtgctt cttcccatct tccttttttc ctttctcctg
     1261 ggaggtggag ccacggctgc agaaaagccc gctcttcccc cttcgcctac ggaacaggaa
     1321 gtggccacgg aaaaatttct ttcccaactc gaggcgactc tggcccaaaa attcttcgat
     1381 tttcgaatcc gtccggtcat ccgggtggcc atattggatt ttaccgacgg agctggcaac
     1441 gtggtgaaag ggggagtgga ctgggccgat cggatcgccc gccggcttta cccccagccg
     1501 cagttcgatt tggtcagccg tgatcgggtg agccaatacc taagctggaa cagcctgggt
     1561 acagtgggga aactggacgc cgccggcttg ctcttgctgc aacgacggat caacaccatg
     1621 gacccgggaa atggcattca tgttttgttg accggggaaa tcaccaaggg caccgggcga
     1681 accatgcagg tccaggccta cttgactaac tttgagcaac gcctggggga acgggagctg
     1741 gagaagaacc tcattgacct gctgacggtg agcggggaag tccccttgcc cacggaacag
     1801 gccttgcaag aggtcagcga agtcgtggtg cgcggagaaa agcatgcttt taccgaaggg
     1861 aggcttttaa ttctggctag tacccgggga tatcctcttg tggagaccga atacctcaac
     1921 cagttcagca aggatcagcc ctttgcctgg gaccaaatcc cctatgccct ggtcgtggga
     1981 aaggaagaat acgcgatgcc caaacaggtc caaatcgggg ctggcaaggt cattcttcat
     2041 ccattgcagg ttccgatgaa ttcgcccaag cgtctggagc attccttcct ccacggcaag
     2101 tgcgccacca atgaggtcta cttcgatgaa atgattcctg ccatgaagta ccaattgacc
     2161 ggctcgttca tcgattttaa gaatggccaa actttttcag agacggccga agtcgaagtc
     2221 tacccgggag ccacaaccgt ggtcatcctg tcgttctttg tccccagcga aaaagagagg
     2281 atccggaacc gaaccttccc cgggatcagc atctaccagc taaacggcaa aggtctggaa
     2341 attttgccga gagggtaaga agacatgggc cttttttcca aaaatgagct gctcggatta
     2401 gatatcgggt cgaactccat taaggccgtt gagctggact ggcgcaagaa aagcccccgg
     2461 cttaaaatct tcgggatgat ccatcttcca cccgaggcca tcgtggatgg agccttcatg
     2521 gattcggcga gcatcgtgga ttccatccgc agcttggtgg agggcctgaa ggttaagacc
     2581 aagaacgtgg ccgtatcgat ctctggccat tcggtgtaca tcaaaaccat caacgttcca
     2641 aacatgtcca aggaacaact cgaggagagc atcaagtggg aggccgagca gcagatcccc
     2701 ttcgacattg aagacgtaaa cattgatttt cagatcctgg acgcccagac cacgccggac
     2761 cagatgcccg tccttctggt ggccgccaag aaggatatga tcaacgatta cacggcggtg
     2821 atcgaagaag ccgggttggc cccaaccatc gtggatgtgg attctttcgc catcgaaaac
     2881 gccttcaatc taaccaacga ttcccctccg gatgaagtgt tggccctggt caacatcggg
     2941 gctagcgtga tgaatatcaa catccttcaa ggggggactt ccgccttcac ccgggacatt
     3001 tccattggag ggcggcagat taccgaagag atccagaaac gtttgaaaat gacctacgag
     3061 gaggccgaag ccctaaaagt caaggaacga gatgcgggag ctcagagcgc ggaagtggat
     3121 aaaatcatcc agggcacggc cgaacagttg gccacggaag tgcggcggtc cctggagttt
     3181 ttcgccgcct cctcttcctt cggggagatc aagaaaatcc atctcagcgg aggctgtgcc
     3241 aagatcccca tgctcccggc cctcatcgaa gagcggatcg gcaccccggt ggaggtcttc
     3301 aaccccttcg ccaagattga atacaacacg gatgagttca gccccgaata catcagagag
     3361 gtgggccctc tggctgccgt ggctgtcggg ctggcccttc ggagggagga cctgaaatga
     3421 tccggatcaa cctcctgccg gtacgcgaga agaagaagaa ggaaagtacc cggaagatgg
     3481 tttcccttct cattctttcc ctggccctgg tcgccgtggt catcaccttc ttccattttt
     3541 ccttgtacag ccaaatcaac gaggtccagc gacaaatcac cgcctacaac gatgagatca
     3601 agcggctgaa gatcgacacc aaggatgtcg acaaattcaa ggccgaaaaa gaagacctcc
     3661 agaggcgaat aaacatcatt tataccctgc agcgggccaa gacggggccg gttcgagtgc
     3721 tggacgatct ggtcacgtcc cttcccggga ggctttggct ggtagccctg cgggagaaga
     3781 gcggcaagat ggaaatcaag ggaatcgcta tggacaacca ggacattgcc aagtttatga
     3841 ccaatctgga gaaatccgga gtcatccgca acgtggaact ggtggtgtcc cagcagttgg
     3901 aaagaaaaga gctgaaactc atggaattca ccctgacctg ccaggttgat tacgccaacc
     3961 tgccagtcgg cattgttaag ggggggtgag agatggcgat caccttggaa agcatcgtca
     4021 aactgcccac ctcccggaag attctcatcc tggcggggat tgtcgcagcc atgatcgggc
     4081 tctatattta cttgatttac tggcccaact tggacttgct gaagaagaag aaagcggaaa
     4141 tgggttctct ggaaaatcag gtgcgggagt tgcgaattgt ggccgccaac atgaaacggt
     4201 tccaggccga ggccgccaaa cttcgggagg aactgcaggt ggcgatcacc caattgccta
     4261 ccagtaagga gatcccgact ctgctgtcca acgtctccca gctggggaaa gacgcggggc
     4321 tggaattctt gctcttcaga cccacggccg aggtgaaccg cgaattctat gctgaaatcc
     4381 cggtagaaat cagggtgaag gggacctacc ataacgtggc cctctttttt gacagggtgg
     4441 ggaggctccc gcgcatcgtg aacatttcag aggtctccat ggagaatgcc aaagagaccc
     4501 ttgggcgctg ggagattagc accgcctgca cggcaaccac tttcaagttc atcgagaaga
     4561 gtcccgctga actggccaaa gaaaaggaaa aaaagaccgg tgaatccaag ggaactccac
     4621 cgaagaagcc ctagagagca cgctatgatg ggaaaaacaa gcatcctttt attgctgggg
     4681 gttttcctgg tcagtgtcgg ttgtggtggg ggagaagccc cgaaaccgac gaaagttccc
     4741 attcctccga cggcgcccaa gccggtggcc aaggtttcgg gcaaagaggt ccctcaaccg
     4801 gctgccgagg ttcccgtcga gccgcccctg gcaacgtcgt atgcttacaa cccgcagggg
     4861 aaagccaacc ccttccagcc cctggtcgtg gagaaaccgt caggcccccc gcccaagaag
     4921 aaagttgaac aggcttccaa aagggagaag gaagagccgg gtacccccct ggagaggctg
     4981 gacctacccg ctctgaagct ggtggctgtg gtttggaata tcccccatcc ccgcgccatg
     5041 gtggaagact ctacggggaa agggtacatt ctctctttgg gaacccggat aggaaagaac
     5101 cacgggcaag tgactcagat tacctcagcc ggagtagtca tcaccgaaaa ggtcgaaggc
     5161 gcggatggga aattgcggac cctagagact ccccttcgct tgtacgcgga tcaatgaagg
     5221 aggaagtatg gaaggaaatc gctggaagat aattttttgt ctcatctctt tgggggcttg
     5281 ggtttttctt tcccttgcct ccccctcctt cgcccagggt ccgcccggac cagcggaggg
     5341 atatgccatt caacggatag aggttaccaa aacggaatcc ggttccagag tatccgttga
     5401 aggctccaag tcattcgagt ataccgtgtt tcgcctggcg aatcctctcc gggtggtgat
     5461 tgatttgccc caggcccagc tgggaaaact ggccggcccg atggaggttc gcgacggcac
     5521 gatcagcgtc atccagaacc ggcaggtggg ggacccccaa aagcgcgggg cccggattga
     5581 gatcggcctg gaacaactgg tggaatatga tgtaaatccc gaggggaagt tcctctacat
     5641 caacttcgga agaccagcca tccccctgcc tcaagtggca aaggaagcgg tgaagaaaga
     5701 ggaaccggcc aaagaatttc ctcccccccc ggccaagccc ttgatgaagg cccggtcttt
     5761 gcggggagtg gatatttcgg cgaaggcgga ttgggtggaa gtggacttga ggggggatgg
     5821 gttgatcccc gaatataata gtttccagct tgcccggcct actcggctcg ctgtagacat
     5881 tcccaagatg accaatgcct cgggcaaggc aaacatcaat gtgggcaacc ggctgctgaa
     5941 agagattcgc atcggacagc acgccgacaa gattcgggtg gtctttacct tcccggacgc
     6001 caaaattccg cagtatcaac tggccaagga gggggccgag ctgaaattga ttttgggaga
     6061 ggtcaagaaa gaagcgccga tgaccgagaa aactccgccc gcagagcctc ctaaaacaga
     6121 gcaggtggca gaagctcctt ccaaagccaa ggaagatgtc gctcctgcgg ccccggtacc
     6181 ggaaaaaccg gcgataaaag aggagaaggc tcctcccccg gaatccccca aacctgtaat
     6241 ggtggcagag gctcttccca aggccaagga ggaagccgct cccacacccc ccgccccgga
     6301 aaaaccctcg atcaaggaag agaaagccct gaacgcggag accaaacctc ccgaaccgga
     6361 gagagctccg gtcggaggat atagaggaag caagatctcc ctggatttca aggatgcgga
     6421 cattcacaac atcctccgcc tcatcgccga agtgagcaat ctgaacatta ttaccagcga
     6481 agatgtg
//
LOCUS       DASPRR010000494         4639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_122592, whole genome shotgun sequence.
ACCESSION   DASPRR010000494 DASPRR010000000
VERSION     DASPRR010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122592"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1135)
                     /locus_tag="VEL68_18120"
     CDS             complement(131..1135)
                     /locus_tag="VEL68_18120"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXZ37946.1"
                     /translation="MKKRGIGISCNTHPAGLKGGGDPSQAQIRLKPDGTLDLLVGAVD
                     IGQGSKTILRQIAADELGVPLEAITVNNFDTDIGPMCTGTFASRVTFMDGNAVIRAAA
                     DFKKQIKEWAAEALEANPADIEMADNKVFVKGAPAKAKTMAEVGGAVNWGGQFIVGTG
                     AFIPGAGQAFDPATGKMTAIASLAFGACVAEVEVDTETGLVEVLKLIQVYEIGKAINP
                     LLCRGQINGGAMMGIGFALTEDFTPYYPSMEHAPDNLADYTIATAADMPREMVWDIVE
                     VPHPNGPRGAKGFSEMTASAAPPAILSAIHNAIGVWVTDYPATPERILRALEEKKAKG
                     "
     gene            complement(1141..2436)
                     /locus_tag="VEL68_18125"
     CDS             complement(1141..2436)
                     /locus_tag="VEL68_18125"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXZ37947.1"
                     /translation="MTEFVGKPMTRYDGIGHVTARTTYVDDIQKPGMLYIKVLTSPVH
                     KGRIRRLDVSAAEKRAGVAGVITAKDVPGANVYGLIADQPVFTPENIRFKGERIAAVA
                     AVDEDEAMEAVEKIKLEIEEQTPVFDPYEAMKPGAPLVRPEGNVFQFDSGPARKIRLG
                     DVEKGFREADYIVEGKYTNGMNDHAPMEPQVSVAYIDETDRLAIHTVSQDLYFHLGML
                     AAVFNLPMNKIRYIGGTVGGGFGAKNDIHCDHVAGLMALKVRKPVKYALTRAEETLYT
                     TKRGAWYFTFKDGVKKDGRIVARQITTLHDTGGYSGMGPYVVDKNSILIGGPFFIPNI
                     WIDGACVYTNKPPASSMRGFGIINGTSAEQLQMDRIAETIGMDPWEIRFINAWRDGDL
                     GATQFQVVAAGLIEAMKRAAELAGVKLPAHLQGMSSRGR"
     gene            complement(2442..2915)
                     /locus_tag="VEL68_18130"
     CDS             complement(2442..2915)
                     /locus_tag="VEL68_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ37948.1"
                     /translation="MKYPLSFILNGLSVDALVKPTDTLLDVLREKLGVTSPKRGCDTG
                     ECGSCTVLLDGEPIRSCLTIALTVAGRKVESVEGLTKQGKLHPLQESFHDHYAAQCGF
                     CTPGMLMSAKGLLDKKAKPSREEIVEAISGNLCRCGCYNEIVEAVEAAAQGPRKG"
     gene            complement(2931..3782)
                     /locus_tag="VEL68_18135"
     CDS             complement(2931..3782)
                     /locus_tag="VEL68_18135"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXZ37949.1"
                     /translation="MKEFLYFAAANLKEAVKLLGNYKKRAVILAGGTDLVPQINTYHF
                     KPEVLVYIGKAGLDYIKEEKGKLIIGAATPMAKIAASKLLARKASALVQAAQQAGTAA
                     VRTAATLGGNLANASPAADLATPLLAMDAELSLVGGKKRVVALKDFFTGPGKTVLKPG
                     EMILEVRFPTSKGKTVFLKLGKRKALSLSVVNTAVRLEMKGQQCREARIAIGSVAPTP
                     LRCPKAEEMLQGKGMDQEIIAACAAEAMAASSPIDDQRASAWYRKKAGVALVARALTQ
                     AAGLQAE"
BASE COUNT          889 a         1411 c         1253 g         1086 t
ORIGIN      
        1 aagtcgataa ttttctttcc cttgaaaagc atttctcgat ccccctcgaa tttcttggag
       61 gggcggggaa ttcccccgcc cccgatgaag tcatgacccc cggcagagcc ggggatttgc
      121 cttaaggata ttagcctttg gctttctttt cctctaaagc cctcaggata cgttcgggcg
      181 tggccggata atcagtgacc caaaccccga tggcattgtg gatggccgac aggatggcgg
      241 gcggggctgc gctcgcagtc atctccgaaa atcctttggc tccccgcggt ccgttggggt
      301 ggggaacctc gacgatatcc cagaccatct cccggggcat gtccgcagcg gtggcgatgg
      361 tataatcggc aagattgtcg ggcgcatgtt ccatgctggg atagtaaggg gtgaagtctt
      421 cggtcaaggc aaacccgatc cccatcatgg cccctccgtt gatctggccc cggcaaagaa
      481 gcgggttgat tgctttgccg atctcataca cctggatcag cttgagaacc tccaccaacc
      541 cggtttccgt atccacttcc acttcggcta cgcaggcgcc gaaagccagc gaggcgatgg
      601 cggtcatctt cccggttgcg gggtcaaagg cctgtccagc cccagggatg aaagctcccg
      661 ttcccacgat gaactgccct ccccagttga ccgcccctcc cacttccgcc atggttttgg
      721 ccttggccgg ggcccctttg acaaagacct tgttatccgc catctcgata tccgccgggt
      781 tggcttccaa ggcctctgcc gcccattcct tgatctgctt tttaaaatcg gctgcggccc
      841 ggatgaccgc gttcccgtcc atgaaggtca cccggctggc aaaggtcccc gtgcacatcg
      901 ggcctatgtc ggtgtcgaaa ttattgacgg tgatcgcttc tagggggact cccagttcgt
      961 ccgcggcaat ctgccgaagg atggttttgg agccctggcc gatgtccacc gctccgacca
     1021 gaaggtccag ggttccatcg ggttttagcc ggatctgggc ctggctgggg tctccgccgc
     1081 ctttcaatcc cgcgggatgg gtattgcaag aaattccgat tcctcttttt ttcatcgctc
     1141 tcaccttcct ctcgagctca tgccctggag atgggcaggc agtttcactc ccgccagttc
     1201 cgccgcccgt ttcatcgctt cgatcagccc ggcagccaca acctggaatt gggttgcgcc
     1261 caggtcgccg tcccgccagg cgttaatgaa gcgaatttcc cacgggtcca tcccaatggt
     1321 ctcggcgatc cgatccatct gcagctgttc agccgaggtt ccattaatga ttccgaaacc
     1381 gcgcatggag ctggccggag gcttgttggt ataaacacag gctccatcga tccagatatt
     1441 tggtatgaaa aaggggccgc caatgaggat cgaattttta tccaccacgt agggccccat
     1501 cccggagtag ccaccggtat catggagagt ggtgatctgg cgggcgacaa ttcttccatc
     1561 ttttttcact ccatctttga aagtaaagta ccacgccccc cgtttcgtgg tatagagagt
     1621 ctcttctgcc cgggtcaggg catacttgac cggtttgcgg accttcaggg ccataagccc
     1681 ggccacatgg tcgcaatgaa tgtcgttctt ggcgccgaat ccgcctccca ccgtccctcc
     1741 gatgtaacgg attttgttca tggggaggtt gaaaacggcc gccagcattc ccagatggaa
     1801 gtagaggtcc tggctcaccg tgtggatggc cagccggtcg gtttcatcga tataggccac
     1861 cgagacctgg ggttccatgg gggcatggtc gttcatcccg ttggtgtact tgccctccac
     1921 gatgtaatcg gcttcccgga accctttttc cacatccccg agacggatct tccgggcggg
     1981 gccgctgtcg aactgaaaaa cgtttccttc cgggcggacc aggggagccc cgggcttcat
     2041 cgcctcatag ggatcgaaga ccggagtctg ctcctcgatt tccagcttga tcttctcgac
     2101 ggcctccatg gcctcgtcct catccaccgc tgccacggcg gcaattcttt cccctttgaa
     2161 acgtatattt tccggggtga acactggctg atcggcaatc agcccataaa cgttcgctcc
     2221 cggaacatcc ttggccgtga tgacccccgc gaccccggcc cttttctcgg ccgcggaaac
     2281 atccaagcgc cggatccttc ccttgtgaac cgggctggtc aggactttga tgtagagcat
     2341 tccgggtttc tgaatatcat ccacataggt cgtccgggcg gtcacatgcc cgattccatc
     2401 ataccgcgtc atgggttttc ctacgaattc ggtcatggct ctcacccctt cctcgggccc
     2461 tgggcagcgg cctcgacggc ttcaacgatc tcgttgtagc atccgcaacg gcacaggttt
     2521 ccggaaatgg cctcaacgat ctcttcccgg ctcggcttgg cctttttgtc gaggaggcct
     2581 ttggccgaca tcaacatgcc cggggtgcag aatccgcact gggccgcgta gtggtcgtgg
     2641 aaagattcct ggagcggatg aagcttcccc tgtttcgtca acccctcgac gctttccact
     2701 ttccgcccgg ccacggtcag agcaatggtc aagcaggaac ggatgggctc tccgtccagc
     2761 aggaccgtgc aggacccgca ttctcccgta tcgcaacccc gcttcgggct ggtgactccc
     2821 agtttttccc gcagcacatc caggagtgtg tcggtcggct tgaccaaagc atccacggac
     2881 aaaccattta gaatgaatga caatggatat ttcataattg ggttccctcc tcactccgcc
     2941 tggaggccgg cagcctgggt caaagcccgg gccaccaggg ctactccggc ttttttccgg
     3001 taccaggccg atgcccgctg atcgtcgatc gggctgctgg cggccatggc ttccgcggcg
     3061 catgcggcga tgatctcttg atccattccc tttccttgga gcatctcttc agccttgggg
     3121 catcgcaggg gggtcggggc aaccgaaccg atggcgatcc gggcttccct gcactgctgg
     3181 cccttcattt ccaaccggac ggcggtattc acaacggaaa gactcaacgc ttttcgcttc
     3241 ccgagtttga gaaatacggt cttccctttg gaggtgggga accgaacctc aaggatcatc
     3301 tctccaggct tcaggaccgt cttcccgggt ccggtaaaga aatccttcag ggcaaccacc
     3361 cgcttcttcc cgccgaccaa actaagctcc gcatccatgg ccagaagcgg agtggccaaa
     3421 tcggccgccg gggaagcatt ggccaggttg ccgccgaggg tcgcagccgt tcgcacggcc
     3481 gccgtccccg cctgctgagc ggcctgaacc agggcggagg cttttctggc caggagcttg
     3541 ctggcggcaa tcttggccat gggcgtggcc gctccgatga tcaatttccc tttttcctct
     3601 ttgatgtaat ccagaccggc ttttccgatg tagaccagga cttccggctt gaaatgatag
     3661 gtgttgattt gagggaccag gtccgttccc ccggcgagaa tcactgcccg tttcttgtag
     3721 ttccccaata gcttcactgc ttctttcaag ttggctgcgg caaaatacaa aaattctttc
     3781 atatccggcc ccctttcggt cgttcagaaa aactccccta acatccctcc aatgaacgaa
     3841 tcttggcttg cttacctccc ttctttttga attccggcgg aagaaccgtt tggccgtttc
     3901 cggccgcccc ccaaatcagt cgaggccacg ggtcatcggc cttacgtcaa gagaaacctc
     3961 cgctcgtggc catattccga ggtcccatga atcctttcat tccttcccgc accggagggg
     4021 catttttcgt tttctagctc ccctttttgc caaggtatca ggtcgtgctc tgtggaggca
     4081 gaaaaaattc cccgccggac ggaatgctgg tagcattcta atacgctggg ccaaccaggt
     4141 caagtgtcct gtcttccttg cccaccccag gtcttaaaaa aaagtccttc gaaaataaga
     4201 cctctggaaa ggagggggga tctcatgggg ttcagggtgg agaaatttgg atcatcatcc
     4261 tgaggcggtg gttcttccat gggatcgggc ctgtcctggg gggattgaaa cggaaaaatg
     4321 ctttggctct ctattctgct tggaggtatc tcctctcagg cctctgcctg cggaacatgg
     4381 ggaggagttt catggaagga taagcacccg gcgagcctga tgatccaaat ttctccaccc
     4441 tgaaccccaa caagcacgaa aaactggggg tgggcaagga agacaggaca aggaagacag
     4501 gacacttgac ctggttgcct cagcgtatta gaatgctacc agtattccgt ccggcgggga
     4561 attttttctg cctccacaga gcacgacctg ataccttggc aaaaagggga gctagaaaac
     4621 gaaaaatgcc cctccggtg
//
LOCUS       DASPRR010000495         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_930019, whole genome shotgun sequence.
ACCESSION   DASPRR010000495 DASPRR010000000
VERSION     DASPRR010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930019"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VEL68_18140"
     CDS             complement(<1..1257)
                     /locus_tag="VEL68_18140"
                     /inference="COORDINATES: protein motif:HMM:NF028510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXZ37950.1"
                     /translation="MWEKIKSWGKWPFRTLRAKLVFSFLLVVLVGGTASIIIGIRMVG
                     NTIIAEAHKKVRHDLASAGIIYQEKLNRIRDVLNLTAKRDLIIREVMGNQVELLKREL
                     ERVRMDYGLDVLTVVDRKGVVLTRTRSPYCSQDFKGNDEMVRRALRKESVASTEIIPQ
                     DELVQEGLDLARQAYMEFIPTPMAKGRPEDKETAGMMMMAAVPILDINNEVWGVIYGG
                     NLLNRNYEIVDKIKDAVFRGERYQGKDTGTATIMQWDLRISTNVRDKNGLRAIGTRVS
                     KEVYDQVLENGRSFIDRAFVVSEWYIAAYEPIRNVKGEVIGILYVGTLEAPYTDLRKN
                     VIYSFLAVAFFGLVLVLFLAYFLTRSITRPIDNLMKATEGIAGGDFSHEVPVESRDEV
                     GHLAASFNRMVRTLRATMEELYVVNTK"
     gene            complement(1384..2250)
                     /locus_tag="VEL68_18145"
     CDS             complement(1384..2250)
                     /locus_tag="VEL68_18145"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXZ37951.1"
                     /translation="MGFQESLAAGKFVITSEIGPPKGTHIQEMMADAELLRGRVDAIN
                     VTDLQSSVMRLGSLAVCRLLQEKGLEPIFQVTCRDRNRLALQADLLSACVLGIKNVLA
                     LTGDYASLGDHPQAKPVFDLDSVSLLGVIKKLEAGTDMVGNALQGAPKFFSGAVVNPG
                     GNPVEAQVIKMEKKIQAGASFFQTQAVYDVGAFEKFMKRVIPFKVPVLAGIILLKSAG
                     MARFMNKNVAGVFVPEPLIQKMAKAEDRVKTSIEIGAELIRELKGMCQGVHIMPIGWE
                     NKVPALLDAAKL"
     gene            complement(2266..2940)
                     /locus_tag="VEL68_18150"
     CDS             complement(2266..2940)
                     /locus_tag="VEL68_18150"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ37952.1"
                     /translation="MLLSQQKSLEEILRSLEGDTKVFLLGCGGCAESSGTGGKAQVLE
                     MKGKLEQAGKKITGHTVIDFLCQKALVQSRLRGKVKEVKEADSLLVMTCGVGVQAVAA
                     VSEKITHPACNTLPLGGSRGEWKGTERCRECGDCVLDSTAGICPLTACSKYLLNGACG
                     GTTKEGKCEVDQEMECGWKLIYERLEKLGRLDKLRRVYEPKDHSKMIPPKGLRLSDRW
                     ALEQGK"
     gene            complement(2962..>4360)
                     /locus_tag="VEL68_18155"
     CDS             complement(2962..>4360)
                     /locus_tag="VEL68_18155"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXZ37953.1"
                     /translation="ITQRICGVCNLCHAMASTLNLDRAFGLADKIPDNGRILRNLAAG
                     LHVVQDHILHFYHLAALDYVDVTKVAKYEGSDPDLKSIKAFIGRGSLGPFVPRYEGDY
                     RLPDDLDRAAVAHYVKALEIRRKGHEAVALLTGRIPHTASIVPGGMTEVPTVDKMASI
                     LWRLNDLKDFVDNVYIPDVLAVAGVYSDYFQVGAGCKNYLSYGAYDLDGKSPDYTTRK
                     RFFQQGTVSSDLKLQPLDLSKITEQVKHSWYDGGTSGLHPSSGKTSPEGTKSGAYSWL
                     KAPRYDGKVYEVGPLAQMLTTYAGGNPQAKSLVDSTLAHFKASPAALFSVLGRHAARA
                     LSAKYLADSMGEWLLQLKPGAPTFVPYEIPDQAQGFGIIDAARGALGHWVEIKDKKIA
                     NYQFVVPTTWNASPRDDGGAPGAIEQALTGTRVKDESNPFEIVRIVRSFDPCIACAVH
                     LVTPRGSEMGRVRIY"
BASE COUNT          775 a         1374 c         1285 g          926 t
ORIGIN      
        1 cttggtgttt accacataga gttcctccat ggtggcccgc agggtccgaa ccatgcggtt
       61 gaaggaggcc gccaaatgac ccacttcgtc ccgggactcc accgggactt cgtgggagaa
      121 atcccccccg gcgatcccct ctgtggcttt catgagattg tcgatgggcc gggtgatgga
      181 acgggtgagg aagtaggcca ggaagaggac cagcaccagc ccgaaaaaag cgaccgccaa
      241 aaagctgtag atcacgtttt tccgcaggtc cgtgtacggg gcctcgagcg tgcccacgta
      301 gaggatgccg atcacttctc ccttgacatt ccggatgggc tcgtaagcgg cgatgtacca
      361 ttcgctcacc acaaaagccc tgtcgatgaa tgacctgcca ttttccaaaa cctgatcata
      421 aacttctttg gagactctcg tcccgatggc cctcagccca tttttgtctc gtacgttcgt
      481 ggaaatgcgc aggtcccatt gcataatagt ggcggtgccc gtgtctttcc cctggtaccg
      541 ctctccccgg aagaccgcat ccttgatctt gtccacgatc tcgtagttgc ggttgaggag
      601 atttcctccg taaatcaccc cccagacttc gttgttgata tccaggatgg gaaccgccgc
      661 catcatcatc atcccggcgg tctccttgtc ctccggccgg cctttggcca tgggggtcgg
      721 gatgaactcc atgtaggcct ggcgagccag gtccagcccc tcctggacca gttcatcctg
      781 ggggatgatc tccgtggagg ccacgctctc tttgcggagc gccctcctca ccatttcgtc
      841 gttgcccttg aaatcctggg agcaataggg ggagcgcgtg cgggtgagga cgaccccctt
      901 gcggtccacc acggtcagca catccagccc gtagtccatg cggacccgct ccagctcccg
      961 cttcaggagt tcgacctggt tccccatgac ttcgcggatg atgaggtccc ttttggccgt
     1021 gaggttgagc acatcccgaa tgcggttgag tttttcctgg tagatgatcc ccgccgaggc
     1081 gaggtcgtgg cggacttttt tatgggcctc cgcgatgatc gtattcccga ccatgcgaat
     1141 gccgatgatg atcgacgcgg tccctcccac caggacgacc aaaaggaaac tgaagaccag
     1201 ttttgcccgc agggtccgga aaggccattt tccccaggat ttaatcttct cccacacggt
     1261 gtactctggc cccccttctt aaactgagaa tagaggggcg gttcaagaag tgccccccgc
     1321 agaaatgata ttttatccct ttgcggggct gctttgcaat tcctcccgag ccgatccggc
     1381 cgatcacaat ttggccgcgt ccagaagggc cgggaccttg ttctcccacc cgatgggcat
     1441 gatgtgcact ccctggcaca tccccttcaa ctcccggata agctcggcgc cgatttcgat
     1501 gctggtcttg acccggtcct cggccttggc catcttctgg atgaggggct cggggacgaa
     1561 gaccccggct acgtttttgt tcatgaaccg ggccataccg gcggacttga ggagaatgat
     1621 tccagccagg accgggacct tgaaagggat caccctcttc atgaattttt cgaaggcccc
     1681 cacgtcgtaa accgcctggg tctggaagaa ggaggctccg gcttgaatct tcttctccat
     1741 cttgatcacc tgggcctcca ccggattgcc tccggggttg accaccgctc cggagaagaa
     1801 tttgggcgcc ccctgcaggg cgtttcccac catgtccgtc ccggcctcca gctttttgat
     1861 gacccccagc aggcttaccg aatccaggtc aaagaccggc ttggcctggg gatggtcgcc
     1921 cagggaggcg tagtccccgg taagggccag cacgttcttg atccccagga cgcaggcgct
     1981 aaggaggtcg gcctgcaggg ccaggcggtt acggtcccgg caggtcacct gaaagatggg
     2041 ctccagcccc ttttcctgca ggaggcgaca gaccgccagg gagcccagcc gcatgaccga
     2101 actctgcagg tcggtcacgt tgatggcgtc cacccgcccc cggagcagtt ctgcgtcggc
     2161 catcatctcc tggatatggg tacccttggg aggtccgatc tcggaagtga tcacgaattt
     2221 tcccgctgcc aagctttctt gaaatcccat gctcaccctc ctcctctact ttccctgctc
     2281 cagggcccaa cgatcggaca gccgcaggcc cttcgggggg atcatcttgc tgtggtcttt
     2341 gggctcgtag acccggcgga gcttgtccag ccttcccagc ttctccagcc gctcatagat
     2401 cagcttccag ccgcactcca tctcctggtc cacttcgcat ttcccttctt tggtcgtccc
     2461 accgcacgcc ccgttgagga ggtatttgga gcaggccgtg aggggacaga tgcccgcggt
     2521 ggaatccagg acacaatccc cgcactccct gcatcgctcc gtgcccttcc actccccccg
     2581 gcttcctccc aggggaaggg tgttgcaggc cggatgggtg attttttcgc tcaccgcggc
     2641 caccgcctgg actcccacgc cgcaggtcat gaccaggagg gaatccgcct cctttacctc
     2701 tttgaccttg ccccggaggc gggactggac cagagccttc tgacagagga aatcgatcac
     2761 cgtgtgaccg gtgattttct tgccggcctg ttccagcttc cccttcattt ccaggacctg
     2821 cgccttcccc ccggtgccgc tcgactcggc gcacccgcca catccgagaa ggaagacttt
     2881 cgtatctcct tccagggagc gaagaatttc ttccagcgac ttttgctgtg aaagtaacac
     2941 gggtttttct cctttccggt cctaatagat gcgaaccctg cccatctcgc ttccccgggg
     3001 ggtgacgagg tggacagcgc atgcgatgca ggggtcaaaa gagcgtacga tccggacgat
     3061 ctcgaagggg ttggattcgt ccttcacccg ggtgccggtc agcgcctgct cgatggcccc
     3121 cggggcgccc ccgtcgtccc tcggagaggc gttccaggtg gtgggaacca caaactggta
     3181 gttggcgatc ttcttgtcct tgatctccac ccagtgcccc agcgcgcccc gggccgcgtc
     3241 gatgattccg aacccctggg cctggtcggg aatttcgtag gggacgaagg tcggcgcccc
     3301 cggtttcagc tggagcagcc actcgcccat ggagtcggcc aggtacttgg ccgaaagggc
     3361 ccgtgccgcg tgccgcccca ggacggagaa gagggccgcg ggggaagcct tgaaatgggc
     3421 cagcgtcgaa tcgaccaggg atttggcctg gggatttcct ccggcgtagg tcgtgagcat
     3481 ctgcgccaag ggtcccactt cataaacctt gccatcgtac cgcggagcct tgagccagga
     3541 ataggccccc gatttcgtcc cctcgggaga ggtctttccc gaggagggat ggaggcccga
     3601 ggttcccccg tcataccagg aatgcttgac ctgctcggtg atcttggaca ggtccagcgg
     3661 ctggagtttc aggtccgaag agacggtccc ctgctggaag aagcgcttcc gggtggtgta
     3721 gtcggggctc ttgccgtcca ggtcgtaagc cccgtaggag aggtagttct tgcagccggc
     3781 tccgacctgg aaatagtcgg agtaaacccc ggccacggcc aggacatcgg ggatgtacac
     3841 gttgtccacg aagtccttga ggtcgttgag ccgccagagg atcgaggcca tcttgtccac
     3901 cgtggggact tcggtcatgc ccccggggac gatggacgcg gtatggggaa tccggccggt
     3961 gaggagggcc accgcttcat gccccttgcg gcggatttcc agggccttca cgtagtgggc
     4021 cacagctgcc cggtccaggt catcgggcag acggtaatct ccctcgtaac ggggaacgaa
     4081 ggggcccagg ctgccccgcc cgataaaggc tttgatggac ttcaggtccg ggtcgctccc
     4141 ctcatacttg gccactttgg tcacatccac gtaatccagg gctgccaggt ggtagaagtg
     4201 gagaatatga tcctggacca catgaagtcc cgccgccagg ttgcgcagga tccggccgtt
     4261 gtcggggatt ttgtccgcca ggccgaaggc cctgtccagg ttgagggtcg aggccatggc
     4321 atggcatagg ttgcagaccc cgcagatccg ctgggtgatg
//
LOCUS       DASPRR010000496         5413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1086524, whole genome shotgun sequence.
ACCESSION   DASPRR010000496 DASPRR010000000
VERSION     DASPRR010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086524"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..527
                     /locus_tag="VEL68_18160"
     CDS             324..527
                     /locus_tag="VEL68_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ37954.1"
                     /translation="MGKIEVNRERCKACGLCISVCPKKLIVFSPNSNSQGFYPAEQSS
                     DGECTGCALCAEVCPDVVIEVWR"
     gene            536..1582
                     /locus_tag="VEL68_18165"
     CDS             536..1582
                     /locus_tag="VEL68_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HXZ37955.1"
                     /translation="MEKRLFMKGNEALACGAIKAGCRYYFGYPITPQNDIPEYFSKHM
                     AKVEGCFIQAESEVASINMILGASAAGARAMSSSSSPGISLMQEGISYLAGSELPAVI
                     VNVTRSGPGLGGISASQGDYFQATRGGGHGDYRTIVLAPHSVQEMHDLAMLAFDLADL
                     YRNPVVILADAVLGQMNETMEDRPYKPMGLPPKDWTLTGASGRKPNLVKSLYLKEGEM
                     ERHNWKLFEKYQSMKKEIRHEVSRVEDAQMIVAAFGTSARVAKSAIQIARSQGLKVGL
                     FRPITLYPFPSQALLDLSQRTKKFISVEMNTGQMVEDVKLSVARDADVHFYGHPPGSP
                     PVPEEVFEAIKNVY"
     gene            1639..2391
                     /locus_tag="VEL68_18170"
     CDS             1639..2391
                     /locus_tag="VEL68_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ37956.1"
                     /translation="MKQVFQRPQSLRDIPFHFCPGCHHGTIHRLVAEAIDHYHLRERT
                     IGVAGVGCAVFIYEYFDIDAAEAPHGRAPAVATGLKRVLRDRIIFTYQGDGDLASIGM
                     SEIVHTANRGENITVIFVNNTVYGMTGGQMAPTTLLGQQTTTSPYGRDFGQDGYPIRM
                     AEMLATLEGTAFSARVAVNTPANLMKAKKAVRQAFEMQMNDLGFTFVELLSSCPTNWG
                     LNPMKANERVERELIPYFPLGVFKERKGSDHF"
     gene            2488..3072
                     /locus_tag="VEL68_18175"
     CDS             2488..3072
                     /locus_tag="VEL68_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ37957.1"
                     /translation="MHQEIIMGGVGGQGIMVIGNLLAQAAFLENLNVTYLPIYGVEKR
                     GGQADCTVVISSDEIGSPVVGYPMSCIIMSRPSLQKYGPRVKPHGFLLLNSSLVDPKE
                     VSRNDLDLLPVPANDLAQEMKNDRLANMIMIGAFVEKTRVVSLDTLISTLPRIFDERH
                     HPLIPANTEAIRKGAEYAWMRLSKYPGKPKTMKE"
     gene            3077..3664
                     /locus_tag="VEL68_18180"
     CDS             3077..3664
                     /locus_tag="VEL68_18180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ37958.1"
                     /translation="MEEEKKSQELRVGERIRELRENKGLSLQDMANRTGYSSALLSQV
                     ENHFISPPLGGLIKIAKALEVKVGTFFGDEPRESYAIVRKDERKHISRFASKEGVSYG
                     YSYESLGFDKKDRQMEPFLVTLEPATVKSEKLSSHDGEEFIFVLEGEMEAILGDHKDV
                     LHPGDCIYYDSTIPHKVQCHREVPTKILAVLWTPQ"
     gene            3679..4251
                     /locus_tag="VEL68_18185"
     CDS             3679..4251
                     /locus_tag="VEL68_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HXZ37959.1"
                     /translation="MSILTSIQAEQEAVLTAGRLMMAAVTTSPKTRGVSTLSSVLIQG
                     EEKDRLARAMEEQFSKKGGRLESFPRDAQNIRRSAAVLLIGVKGILPKKPENPLNCGA
                     CGYLSCAQFIRAEKKRGEDFAGPLCAFQVMDLGIALGAAAKMAAEFNIDNRIMYTAGA
                     AAMTLGLLEADLIVGMPLSISEKNIFFDRK"
     gene            4382..5233
                     /locus_tag="VEL68_18190"
     CDS             4382..5233
                     /locus_tag="VEL68_18190"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HXZ37960.1"
                     /translation="MNLQDQILKCARSCQAPNQKTLATISIQDRDSLASTLGVSKRAV
                     EIAALDLEVWPQRYLRNMGSLGISGQKKLLQARSLLIGVGGLGGTIAILLARMGLGSL
                     VIADGDSFSEDNLNRQALSLEPNVGAKKVRAARMEIARINAATEVEVFEGFVAEKELS
                     NLIQGAQVAVDGLDNMPSRFRLEKACKDAGVPLVHGAVAGFTGQVSTIYPEDPGFRAF
                     YGDPQNAPEKGVEVELGNLAGIVSTIASLQVQEAIKVLTGLGRPLRNRLLFIDSLNGV
                     SETISLK"
BASE COUNT         1227 a         1559 c         1498 g         1129 t
ORIGIN      
        1 ttccgctgtt cgtggtctag attaaacacc aattccctta aaatcacaag agttccgttt
       61 attagttgct ggttgcgggt tgctggtttt ctcctttaac cagtaacgag taaccagcaa
      121 cggtctttca ttccgcattc cacattccga attccgcatt tgtaatgatc ccactttcct
      181 cttgctcaat cgatgttcgc tgtgttaatt taatactaag tggagaattc ggaagggatg
      241 ggagctttga gtagtcgatc ttctctcagg tgtcccccca aaacctccct ttttcaattt
      301 aggtcaacct ggggtccagc aaaatgggaa aaatcgaggt caaccgcgag cgatgcaagg
      361 cttgcggcct gtgtatttct gtttgtccga agaaactgat cgtcttcagt ccaaactcca
      421 acagccaggg cttttacccg gccgagcaaa gttcggatgg ggaatgcacc ggttgtgccc
      481 tctgcgccga ggtctgcccg gatgtcgtga ttgaggtctg gaggtaggga tcacggtgga
      541 aaagcgtctg ttcatgaaag gcaacgaagc cctggcctgc ggggccatca aggccggctg
      601 tcgatactat ttcggctacc cgattacccc ccagaatgat atccccgaat acttttccaa
      661 gcacatggcc aaggtggaag ggtgcttcat ccaggcggaa agtgaagtgg cctccattaa
      721 catgatcctg ggcgcttccg ccgccggggc aagggccatg agctcttctt ccagcccggg
      781 gatctccctg atgcaggagg ggatctccta cctggccggc agcgagctgc ctgcggtgat
      841 cgtgaacgtc acccgcagcg gaccgggcct gggaggaatc tctgcttccc agggagatta
      901 tttccaggcc acccggggcg gaggccatgg ggattatcgg accatcgtcc tggctcccca
      961 ctcggtgcag gagatgcatg acctggccat gctggccttt gacctggcgg acctttaccg
     1021 caacccggtg gtgatcctgg ctgacgcagt tttgggacag atgaacgaga ccatggaaga
     1081 tcgtccttac aagcctatgg gactcccccc taaggactgg accctgaccg gagcttccgg
     1141 aagaaagccc aacctcgtca aatccctcta cctcaaggaa ggagagatgg agagacataa
     1201 ctggaagctc ttcgagaagt accagagcat gaaaaaagaa attcgccatg aggtttcccg
     1261 ggtggaggat gcccaaatga tcgtcgccgc tttcggaact tctgcccggg ttgccaaaag
     1321 cgccatccag atcgcccggt cccaggggct caaagtgggg cttttccggc cgatcaccct
     1381 ctaccctttt cccagccaag cccttctgga cctctcccag cggaccaaaa aattcatctc
     1441 cgtcgagatg aacaccggcc agatggtgga ggacgtaaaa ctatccgtgg cccgggatgc
     1501 ggatgttcat ttctacggtc accccccggg ctctcccccg gtcccggaag aggtcttcga
     1561 ggccatcaaa aacgtctatt gagggagcct cagaatgagg ggggagaact cccccgtgga
     1621 aaggtgttaa gacccgatat gaagcaggta tttcaaagac cccaatcgct gagggatatt
     1681 ccctttcatt tctgcccggg atgccaccat ggcacaatcc atcgcctcgt ggccgaagcc
     1741 atcgaccatt accatctccg cgagcggacc atcggggttg cgggggtggg ctgcgcggta
     1801 ttcatttacg aatattttga cattgacgcg gccgaagctc cccacgggcg ggctccggcg
     1861 gtggctaccg gcttgaagcg ggttctccgc gaccggatca tcttcaccta ccagggagac
     1921 ggagacctgg cctccatcgg aatgtcggag atcgtccaca ccgccaaccg gggagagaac
     1981 atcaccgtca tcttcgtgaa caacacggtc tacggaatga ccgggggcca gatggcccca
     2041 acaacccttt tagggcagca gaccacgacc tccccgtatg gaagggattt cgggcaggac
     2101 ggatacccga ttcgcatggc ggaaatgctg gccaccctgg aagggacggc cttttccgcc
     2161 cgggtggcgg tgaacacccc ggccaatctg atgaaggcta aaaaggcggt ccgccaagcc
     2221 tttgaaatgc agatgaacga tttgggtttc acctttgtgg aacttctctc ttcctgtccc
     2281 accaactggg gcctaaaccc gatgaaagcc aacgagcggg tggagagaga gctcatccct
     2341 tacttccctt tgggggtctt caaggagcgc aaagggagcg accacttcta aaattgcgga
     2401 ttgtggattg cggattgcgg attttgaaac ctgccttttc aatccgcatt ccgaaatccg
     2461 caatccgaaa tcatgagcgg agcggatatg catcaggaaa tcatcatggg gggggtcggg
     2521 ggccagggca tcatggttat cggaaatctc ctagcccagg cggccttcct ggagaatttg
     2581 aacgtgacct accttccgat ttacggagtg gagaagcgcg gggggcaggc cgactgcacc
     2641 gtcgtcattt cctccgacga aatcgggtct cctgtggtcg gttaccctat gtcctgtatc
     2701 ataatgagcc ggccttccct gcagaagtac ggtccccggg tcaaacccca cggttttctt
     2761 cttctaaata gctccctggt ggaccccaag gaggtgtccc gcaacgatct ggacctcctt
     2821 ccggttccgg ccaacgacct ggcccaggag atgaagaacg accggctggc caacatgatc
     2881 atgatcggcg ccttcgtgga aaagacccgc gtcgtcagcc tggacaccct gatttccact
     2941 cttccccgga tcttcgatga acgacaccac cccctcatcc cggccaacac cgaagctatc
     3001 cgcaagggag ccgaatatgc ctggatgcgt ctttccaaat accccgggaa acccaaaacc
     3061 atgaaggagt agtccgatgg aagaggagaa gaagtcccaa gagcttcgag tgggagaaag
     3121 gatccgcgag ctcagggaga ataagggcct ctctttgcag gacatggcca atcgcacggg
     3181 gtactcctcc gccctccttt cccaagtgga aaatcacttc atctcgcccc ccctgggagg
     3241 tctgatcaag atcgccaagg cgctcgaagt caaagtaggg accttcttcg gggatgagcc
     3301 ccgcgaatcc tacgccatcg tgcgaaagga cgagcgcaag cacatctccc ggtttgcttc
     3361 caaagaaggg gtcagctacg ggtattctta cgaaagcctg ggctttgaca aaaaggaccg
     3421 gcagatggag cctttcctgg tcaccctgga gccagccacg gtgaagagcg aaaaactttc
     3481 ctcccacgac ggggaggaat tcatctttgt cctggaagga gagatggaag ccatcctggg
     3541 ggaccataag gatgttcttc atccgggaga ctgcatctat tacgattcca ccattcccca
     3601 caaggtccag tgccaccgag aggttcccac caaaattctg gccgtcctct ggacccctca
     3661 gtgaaggagg gtaaattatt gtccattcta acctcaattc aggctgaaca agaagctgtt
     3721 cttacggccg gccggctgat gatggccgcc gtcactacct cgccgaaaac ccggggagtc
     3781 agcactcttt cctcggtcct gattcagggg gaagaaaagg atcggttagc ccgggccatg
     3841 gaggagcagt tttccaagaa gggaggccga ctggaatctt ttccacggga cgcccagaac
     3901 atccggcggt ctgccgcggt cctgctcatc ggggttaaag ggatcctgcc caagaagcct
     3961 gaaaacccgc ttaattgcgg agcctgcggt tacctcagtt gcgcccaatt catccgcgcg
     4021 gaaaaaaaac ggggcgaaga tttcgccggg cccctctgtg ccttccaggt catggacttg
     4081 ggcattgcct tgggcgcagc agcaaagatg gccgccgaat tcaatatcga caatcggatc
     4141 atgtacaccg ccggggccgc ggccatgacc ctggggcttc tggaagccga tctgatcgtc
     4201 ggtatgcccc tctccatcag cgagaagaat attttcttcg accggaaata aaaaaagctg
     4261 tcggtgatac ggacaccgca ataaaaacag gttccgagtt tcgggttccg cgtgaagaac
     4321 cggaattggg ggaagaggtt ttaacccgaa acttgaaatc cgaaacccga aactggtttt
     4381 catgaatctc caagatcaaa tcctgaaatg cgcgcgatcc tgccaggctc ctaaccagaa
     4441 gacccttgcc accatctcca tccaggaccg ggactcactc gcttcaactc tcggggtgtc
     4501 caagcgggcc gtggaaatcg ccgccctgga cctggaggtt tggccccagc gttatttgcg
     4561 caatatgggc tccctgggga tttccggcca gaagaagctc ctccaggcca gatccctgct
     4621 gatcggggtc ggagggttag ggggtaccat cgccatcctc ctcgcccgca tgggtttggg
     4681 tagtctggtg attgccgacg gggattcatt cagcgaggac aacctaaacc gccaggccct
     4741 ctctctggaa cccaatgtgg gcgccaaaaa ggttcgcgcc gcccggatgg aaatcgcccg
     4801 aatcaacgcg gccacggaag tggaagtctt cgaaggattc gtggccgaaa aagaactttc
     4861 caacctcatc cagggagctc aagtcgccgt cgacggcctg gacaacatgc cttcccgttt
     4921 ccgtttggaa aaggcgtgca aggacgcagg ggtccccctg gttcacgggg cggtagccgg
     4981 gtttaccggg caggtgagca cgatctaccc agaagacccc ggttttcggg ccttctacgg
     5041 ggacccccag aacgccccgg aaaaaggagt cgaggtagaa ttgggaaacc tggccgggat
     5101 tgtctccacc atcgcttccc tccaggtcca ggaagccatc aaggttctta ccggcctggg
     5161 ccgcccgctg cgcaaccgcc tcctcttcat cgactcgctg aacggcgttt ccgaaacaat
     5221 ctcccttaag tagtgtccat tcgcaaatcc tgattccccc ctgcccggca atgatagctc
     5281 tgtttctttg aacggaaacc ccatcaagcg tgaagcggtg gagagaaatt ttgtcattga
     5341 atttccagct tccatcttcc agtatttgaa aagagcgttc tccaaatcga gcggatggaa
     5401 agggggccag gcg
//
LOCUS       DASPRR010000497        39909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_774339, whole genome shotgun sequence.
ACCESSION   DASPRR010000497 DASPRR010000000
VERSION     DASPRR010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 39909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39909)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39909
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774339"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VEL68_18195"
     CDS             complement(<1..585)
                     /locus_tag="VEL68_18195"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HXZ37961.1"
                     /translation="MKVSFLRAILFLFLLGTGGHSLASSDFPWQKVGEGFDYKFFRVE
                     GLPYQSLVKLTILRIGWDQFQVRILDSRSHGADRMEIKALARKAQALAGINGGFFLPG
                     YRPLGLLIVDGKEANPLRKADWGIFLVQDDLPRIIHTTEYQNGWNISQALQVGPRLVV
                     NGRELQMKKQVARRSALGVTMKNQVILLTSEDTDV"
     gene            1005..1775
                     /locus_tag="VEL68_18200"
     CDS             1005..1775
                     /locus_tag="VEL68_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXZ37962.1"
                     /translation="MLLFAKDRNARERGNQTLNPLIQKGPAKRLSVVIPVFNEAGTIG
                     EVIDRVQAAALPEGLEREIIVVDDFSTDGTREILRQYGSTDSFRLFLHDRNLGKGAAL
                     RTGFAQTTGDIVLVQDADLEYDPREYGKLLEPILEGKADAVYGSRFMGGDPKRTLLFW
                     HYLGNRFLTTLSNLFTNLNLSDMETCFKVFRGDLIRKIKTHENRFGFEPEVTSKIAKS
                     GARVYEVGISYSGRTYEEGKKITWKDGVRAIWTILRYH"
     gene            1944..2798
                     /locus_tag="VEL68_18205"
     CDS             1944..2798
                     /locus_tag="VEL68_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXZ37963.1"
                     /translation="MARVNGNKDYYRILGVSREAAPEEIKKAYRQLALKFHPDRNPGD
                     GQAEEQFKEISEAYGVLMDPDKRKQYDLLSRFPFEAGRRPDFSYTQEEIFRDIFNNPQ
                     ASDVFSELGREFARMGFRFDERFFDHLFFGGQGFFFILGGPGGFRYSSSGGSQEKTRN
                     AAEVFSPILENVLVPRGGTLTGRIASWVFRKGIGFFLSRLMPNPSPSGPKGDNFLDLT
                     YTLPLSRTEVSTATTREFSFQRNGKQEKLLVKIPAGIRDGTLLRLRGRGKNQGGKVGD
                     LYLRIRLT"
     gene            complement(3221..3757)
                     /locus_tag="VEL68_18210"
     CDS             complement(3221..3757)
                     /locus_tag="VEL68_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37964.1"
                     /translation="MGKFFMISLILVTMVLSFYSLSFADEEAKGIFPTNSLNELIYGK
                     WLLQRKGQDPFSSSPKAGLGSTLNGAKPGKAIKNREHPLGPEITDPMHYPAATEIQER
                     ELFHSENSQPSKEKSGYHFGKIHLTKNNLDPSLDFISRLSVAQFKEESPPSWTLEKFK
                     ENDIFKSLVIFLELKLNF"
     gene            4423..4854
                     /locus_tag="VEL68_18215"
     CDS             4423..4854
                     /locus_tag="VEL68_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXZ37965.1"
                     /translation="MKAKKFVLGAIATIVVGWIAFGADVSVGQEKKTRVYELRTYTVL
                     PGRLPALHKRFAEHTMKLFEKHGIRNEMYWVPIDDARKDNTLIYVVSHESMEAADRSW
                     KAFYADPEWIKVRDASEADGKILAKPPERVFMKLTDYSPSR"
     gene            complement(5164..6537)
                     /locus_tag="VEL68_18220"
     CDS             complement(5164..6537)
                     /locus_tag="VEL68_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HXZ37966.1"
                     /translation="MLSSEEIIRTVEMLKNENLDVRTVTLGISLRDCASPSADEVCGR
                     VREKVRRHARNLVPTCERVATKYGIPIVNKRLAVTPIASVAESLRGEDFLMVARTLDQ
                     AAQEVEVNFIGGYSALVQKGFTRGDRTLLDTIPEVLSSTERVCASVNVASTRAGINMD
                     AVSLMGQAIQETSQRTADRDGFGCAKLVVFANMPEDNPFMAGAYLGYGEPECVINVGV
                     SGPGVVKRELERAVQSGKKLTLGDLSEIIKKTSFRVTRVGELIAREVAREMDVSFGIV
                     DLSLAPTPTVGDSVGEILQCLGLESIGVPGSTAALALLNDAVKKGGAFASSSVGGLSG
                     AFIPVSEDLNISRAVQQGYLTLEKLEAMTAVCSVGLDMIALPGKVDADTIAALLADEM
                     AIGVINHKTTGVRLIPVPGKDVGDKAVFGGLFGEAYILEVRNINRSGNFVRFGGRIPA
                     PLMSLNN"
     gene            complement(6631..7206)
                     /locus_tag="VEL68_18225"
     CDS             complement(6631..7206)
                     /locus_tag="VEL68_18225"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXZ37967.1"
                     /translation="MNDDPRMEWVAITVIGRDRPGIVANVSGVLYRHRSSIEELSQTV
                     IRGQFAMILIASLGEGASVPALKGELRDLSRDLDLDIDLRRLKPEDLIPYQSGETEPF
                     IITARGKDRPGLVYGITELLAERGINITNLGAQVARLGQELEYIQVFEVDIPKNLDYG
                     LLQEKIHQRGQEMGVSVDLQHKDIFRAINQI"
     gene            complement(7253..8167)
                     /locus_tag="VEL68_18230"
     CDS             complement(7253..8167)
                     /locus_tag="VEL68_18230"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HXZ37968.1"
                     /translation="MMIPEFPQFRLIQLEDREVIGEIIWRYQPQTSEWTFSNLFIWRS
                     YYGFQWSLYRDWLLVLSTAGRQGAFFLPPIGPPSRLEVVQESLKWLKEEKGKKNPRIE
                     RADSRLVDEIQDSPDLVAEPTREQFDYLYRSQDLIQLAGRKYHGKRNHINKFLQTYAF
                     TYSPLEERHLPECLELGGFWCEVRRCEGDMNLMGEWEAVREALTHFTKLRIQGGVLLI
                     QDKVQAFSLAELLNRETAVIHLEKANPEIPGLYPMINQQFCEKSWSRVLWINREQDLG
                     EPGLRKAKESYFPDHLVDKFRIRMVERA"
     gene            complement(8319..8924)
                     /locus_tag="VEL68_18235"
     CDS             complement(8319..8924)
                     /locus_tag="VEL68_18235"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXZ37969.1"
                     /translation="MRSRAAAAVLAREGFEEVYSMEGGIHAWKGLRATGAPDAGMAYF
                     EPARKPEELIALAWMLEEGSRKFYKEMGTKEKIPGAVDLFQELSVDEEKHKSSLFKIY
                     QRISRKASDPGFPRSLVSLETGDDYLEGGMGLKEVLEWSRGKELKDILEFSISLEANA
                     IDLYIKMERRIEGTEAKEVFLALSNQERNHLKRLSAALENS"
     gene            complement(8934..9155)
                     /locus_tag="VEL68_18240"
     CDS             complement(8934..9155)
                     /locus_tag="VEL68_18240"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXZ37970.1"
                     /translation="MGILDFFKPVSTWPPDRVRQFLDEKSPQEYNLVDVRTPKEYEQE
                     HLPGARLIPVAELETRWKELDRNKPTITY"
     gene            complement(9217..9738)
                     /locus_tag="VEL68_18245"
     CDS             complement(9217..9738)
                     /locus_tag="VEL68_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37971.1"
                     /translation="MGKHLVLVGGGHAHLTVLFNTSHFVQRGHRVTLIGPTPYYNYSG
                     MGPGMLSGIYQPREVRFHGKKMMEDRGGIFVEDRVIRINSNRRTLNLRSGKQIDYEVA
                     SFNTGSEVPLKFLVSASRENIVPVKPVINYGQRAGDSGEEEFCVGRANGFFAEGLHRS
                     KVHGKISGVRGKG"
     gene            complement(9824..10849)
                     /locus_tag="VEL68_18250"
     CDS             complement(9824..10849)
                     /locus_tag="VEL68_18250"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HXZ37972.1"
                     /translation="MKVHVLLLFFLWGLLEMGGFRVAMGSDAWPGRVKEVEDHRWEID
                     PTESEAYGPDFFQNVYLEWNKPAFFGAQIFSWLRSAEDPSQPLFFAQASLGKSEHRYT
                     LVDEGGAPLSESSQKESSKPQRDWKGIGIDTAFFLGYQVVFAGVLWFLPESVTSWTED
                     QKKATFAKWKVNVQNPVWDHDKFWINYIAHPYFGATYYTRARERGFGEFGSFWYSALL
                     SACYEFGIEAFFEPPSKNDLIVTPVGGYIVGKYVFEPIRDVIKAKPELKWYDHAGLIL
                     TDPLGALNSVFYWLFGIQSDIRVQFHSPVLARQVGVDPSEERSFMGQEVKFSRPQGVN
                     LQLNLDW"
     gene            11027..12040
                     /locus_tag="VEL68_18255"
     CDS             11027..12040
                     /locus_tag="VEL68_18255"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HXZ37973.1"
                     /translation="MHKGVPFCVGVAIGWGFLFLSPYLHAVEMTPFYTQNQSPLVQIF
                     GLPSIGEASVVPSQKADLRLIVDLANNFVNDKTSNESIVLDGQSTRFSLDARYGIAKN
                     FEVGLVIPYIIEGGGFTDGFIDWYHNTFGFPAGGRDHAPKNRLLYQYQKDQQVRLNVD
                     QSSSGIGDIQLTGAFQLYRNPDKPSQAISLRTSLKLPTGDSQQLHGSGSADLSLWATA
                     GDDYKISVGHFTLYGAAGIMGMTNGDVLKEQQRNWVGFGCLGGGWSPARWIAFKLQAN
                     GHTSFYKDSELRELNMNSIQLTIGGTLAFSERISLDVGVTEDVVTLTSPDVVFHLALR
                     GTF"
     gene            complement(12210..12857)
                     /gene="eda"
                     /locus_tag="VEL68_18260"
     CDS             complement(12210..12857)
                     /gene="eda"
                     /locus_tag="VEL68_18260"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXZ37974.1"
                     /translation="MNEVLSKIVDEGVVPVIRVASAGEAFEVAKAIRDGGISVIEITF
                     SVPGALDVMKEVTQKFGKEVLLGAGTVLDPETARAALLSGAKFIVTPTLNLDVIKMCK
                     RYSAVVIPGALTPTEILTAWEAGSDQVKVFPIAQVGGPAYIRALKGPLPHIPLVPTGG
                     VNLQNAGEFIKAGAAAIAAGGELVDKKAVAEKKFSVIAENAQRFLGEVKKARGKG"
     gene            complement(12906..13892)
                     /locus_tag="VEL68_18265"
     CDS             complement(12906..13892)
                     /locus_tag="VEL68_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXZ37975.1"
                     /translation="MMYDVVTFGEAMLRLSPPHFARLEQTHSLDVEIGGSELNVAVGV
                     ARLGMASAWVSKLPNNALGKMVRNRGRELGVDTSHVVFSDQGRQGLYFVEYGAAPRAS
                     SVLYDRANSAISLIQSNEVDWKKILSKARLFHVSGITPALSPSAAETTATAMKAAKEA
                     GCLVSYDLNYRKKLWSPADAKKCQEPLMKLVDIITTTEEDTGVVFGIREQNYQKAAET
                     LSRTFGFKAVAITLREDLSVWKNNWTAIAYADGKVYSDRTYAVEIVDRVGAGDSFTAG
                     FIYGYLTGGAEKGVKYGNALSALKHSMPGDFNWSTLEEVEAQIKGAGLRISR"
     gene            complement(14076..15083)
                     /locus_tag="VEL68_18270"
     CDS             complement(14076..15083)
                     /locus_tag="VEL68_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ37976.1"
                     /translation="MNGETILKVEGLKKFFPIRRGVLSSVVGHVRAVDGVDLEIKGGE
                     ALGLVGESGCGKTTIGKTLIRLYQADAGRAVYDGLDVFALKKQELKELWKRMQIIFQD
                     PYSSLNPRMTIQRIVGEPLRRFHLVPASRIKEVVAEIIARIGLNTDHLERYPHEFSGG
                     QRQRIGIARALVGRTVTDYPKMIICDEPVSALDVSIRAQILNLLKHLQKEFHLTLLFI
                     SHDLSVVEHVCDRVAVMYLGRIVEVAGRDDLYQNPLHPYTQAFMSAIPTPDPNAEGLQ
                     KRIILKGDIPSIIHPPNGCYFHTRCTCAFERCRKEYPELRRAAEGHRVACHLMKESKQ
                     S"
     gene            complement(15213..16199)
                     /locus_tag="VEL68_18275"
     CDS             complement(15213..16199)
                     /locus_tag="VEL68_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ37977.1"
                     /translation="METLLRIRDLRTYFFTLSGIVRAVDGISFDLAPGETLGLVGESG
                     CGKSVTALSIMRILPEPAGRIAEGSILFRGKELTGLPEKEMEKIRGEKIAMVYQEPMT
                     ALNPAFTIGDQLGEVFRFHQGLGKREALEKAAHLLDQVGMPSPASRIKDYPHQLSGGM
                     RQRAVIAMAMACNPEILIADEPVTALDVTIQAQILDLILKLRSRMGMSLILISHDLGV
                     VANMVKRVIVMYAGRIVESAGVGEVFRSPLHPYTRGLFQSIPRIGRRVDTLPEIPGSV
                     PNLLNLPAGCKFANRCPEAMDRCRMEEPHLFEPGRDHFSRCWLNECGIGNAP"
     gene            complement(16351..17175)
                     /locus_tag="VEL68_18280"
     CDS             complement(16351..17175)
                     /locus_tag="VEL68_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37978.1"
                     /translation="MFLRRLIRNKLAVLGAVLLLFLVAMAVLAPWVATHNPFKLNIKE
                     RLQPPSSSHVFGTDGYGRDLFSRVVYGAAIALRVGFLSIIVAMTAGILLGLISGYYRG
                     WIDTVVMRIMDAILSFPIILLAIGIMAVLGAGFANLMIALGVVYTPRFARLVRSSVLS
                     IREKEYIEAARVTGCSDIHILFVHILPNCWAPVIIQATISFGYAILWEAALSFLGLGA
                     PPPSPSWGSLLADGKEVLSRAPWLTYFPGAAIALAVLSLNLFGDGLRDVLDPRFKE"
     gene            complement(17181..18128)
                     /locus_tag="VEL68_18285"
     CDS             complement(17181..18128)
                     /locus_tag="VEL68_18285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ37979.1"
                     /translation="MKTYVIKRLLQVIPVLFLIAGIVFFLLRMIPGDPAAIYVGTNAE
                     PGVVESIRREMGLDQPIYVQFFIWLSRIIRGDFGASIFYHLDVIQLILQRLPVTLTLG
                     FLSMVFALLLAVPSGIMASVNQNNYKDYLFTLLAVMGISIPGFWLALMLVIFFSVKLG
                     WFPATGYVSPFENFLKGMHYLILPSISQALLASAIIARMTRSTMLEVLRQEYMTTARG
                     KGLPKVWVVGKHALKNAFAPTLTVIGFQLGNILGGAVITETIFSLPGIGQLIYTAIGQ
                     RDYPIIQGCILLTAFGFVIINLIVDLLYSYFDPRIVFEG"
     gene            complement(18232..19797)
                     /locus_tag="VEL68_18290"
     CDS             complement(18232..19797)
                     /locus_tag="VEL68_18290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ37980.1"
                     /translation="MIRFEKWGVLWVVFIGAMIVGGLSFAQQPKYGGTLVVGTVTDIV
                     GIDPHKVTGEISMIVLNNIYERLVDLDEKNMPMPGLASKWSVSPDGLVYTFHLRQGVK
                     FHNGREMNADDVAYSYSRLMDPKTKYPFVTQVQNIKDIKVVDKYTVQFTLKAPTITLM
                     VYMSNPYGTHAIVPKEEVEKQGGTLTRPVGTGPYKFVEYIPDKHVIIERNKDYQGSNL
                     PTSGTGGMKPAYADRIKFVPIKDAAVRSMALKSGDIDFTLRVAWEEIDEFKKDPNIVT
                     YVSPGLAYVMFMFGVNTSTNKFIRMADFRRAVGHCLDLQELVDGSVFGYSLVNPSLVN
                     VVDPFYSDVHKKGYGKDLKKAKELLKKVGYDGTPVKIAVTKVYLNTYKTAILAEQMLG
                     EAGIKVQLDVKEWPALLQDYNAGKHDILSFIASGMTDPCLNMVRVHSKRNFVGYKNEK
                     VDALVEKAEATSDFATRKKLYEQIHEIMLEDVPLLKLYDHSVAPATRKYVKGFKPMPY
                     TADARLWHVWLDK"
     gene            complement(19966..21873)
                     /locus_tag="VEL68_18295"
     CDS             complement(19966..21873)
                     /locus_tag="VEL68_18295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HXZ37981.1"
                     /translation="MGSRFEKIGQFWHVIFVVFSSLGILLAVNQLFRLELFGFNPLDT
                     SYLYYNLTLYLSTAFLLYPAAKSAGDKVPWYDVVLFSVVVIINIYFGIHGIDIIERGW
                     EYRSPLPATIFSVLLWVLVMEAVRRSTDIYMTLMCLIFSAYPLFAVYLPGFLQGQSHD
                     FLTSARLYAMGSSGILGIPLDVVCTLLIGFMVFGVVLQNTGGGTFFFRLAQSLLGHTR
                     GGAAKISILASAFFGMISGSTTSNTLTIGAMTISAMKSTGFPPHLAAAIEACASTGGP
                     IMPPVMGAAAFIMASFLNVPYAVVAIGAFIPACLYYLGLFVQVDGYAAKANLKGLPKG
                     ELPSFWGTLKEGWFYTLAIVLLVYFLLTLRLEARAPYYASAALVALSMIKKESRLDWK
                     KFIAMIANIGKVVTQLVAILAAAGLIIGGLSVTGVALAFSRELVGAVGDNIFLILLMG
                     AFSSFVLGLGMTITACYIFLAIVMVPALISLGIDPLAAHLFVFYWGVLSEITPPVALC
                     VSAACGLAGSNFMQTGWAAMRLGAVKYILPFFFAYNPALLTHGVWWEVLLVTFFAVVG
                     IYYLGSALEGYLWGIGQINWPIRISLITGGLLIAFPEIYTTLIGLAIVGCVNLICYFT
                     SKGRPKFGVSP"
     gene            complement(22030..23178)
                     /locus_tag="VEL68_18300"
     CDS             complement(22030..23178)
                     /locus_tag="VEL68_18300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ37982.1"
                     /translation="MKKAKARWVVLGLFLGGLLFVAGLSPAAEKGSGLPGMMTWTAFD
                     VGSRGYVQGAAISNALTKKYATKVRILPSGTSVGRLIPIKTGAATYGLLADETYFAAE
                     ALYEFSFPNWGPQDLRVLLAHPAPIGMAATAKSGIKTLKDCKGKKIGWIPGASTNNVK
                     TEAFLAFAGLTWKDVQRIDLPSYAAAGKGLIEGKIDAISYGITAPLLYELEASPQGIS
                     WPEFPASDVEGWKRLHQLTPWLSPGKYDIGPGLKKGQPKEIPSYTYPQLVCYAKQDAN
                     EVYALVKGFDETFDMYKDADPELPEWAIARSGRVPAGAPFHEGAIRYLKEKGIWTAQS
                     DQWNRKFLDRLKKVQGAWKTAMQEATAKGMPEKDFTEFWLKKRGEITE"
     gene            complement(23291..24124)
                     /locus_tag="VEL68_18305"
     CDS             complement(23291..24124)
                     /locus_tag="VEL68_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXZ37983.1"
                     /translation="MDKLIITAAVTGSGPTRKNNPNIPYTPEEIANEIVRSFEAGASI
                     AHVHVRDPKTGAPSFELDYFREVRDRVRSRCNILLNFTTSAANLTGDNLIERRLGTTT
                     LGPELCTLDVGSMNYQNRVFINPPEWGPIGAKVARERGVKPELECFDAGHVRIAVSLI
                     EKGVVDPPYLFQICLGVEGGMDAGIRQFLFMKDLLPTRDVVWTTLGIGRHEFSTVTLS
                     ILNGGHARVGFEDNVYLSKGVLAKSNAELVEKVVRLARELGRDIAQPDEVRQMLGIGK
                     K"
     gene            24580..24744
                     /locus_tag="VEL68_18310"
     CDS             24580..24744
                     /locus_tag="VEL68_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37984.1"
                     /translation="MPKHRGAYKSEKRRKELTRQKKQEERRQRKFSKGDHPPSDAEKV
                     EQEENQPANP"
     gene            25068..25400
                     /locus_tag="VEL68_18315"
     CDS             25068..25400
                     /locus_tag="VEL68_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXZ37985.1"
                     /translation="MAKKLFVGGLSWDTTDDGLKNAFAAHGEITEAKVITDRDTGRSR
                     GFGFVTFTQDEDAKTAIAKMDGTSLDGKTIKVNEAQEKSPRGGGRPGGGRGGGFGGGG
                     RGGGRDRW"
     gene            complement(25637..27472)
                     /gene="typA"
                     /locus_tag="VEL68_18320"
     CDS             complement(25637..27472)
                     /gene="typA"
                     /locus_tag="VEL68_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXZ37986.1"
                     /translation="MKRSAQNATLRNIAIIAHVDHGKTTLVDQMFKQSGVFRPGQEVA
                     ERVMDSLDLERERGITIAAKNCSVSWQDVKINILDTPGHADFGGEVERALMMVDGALL
                     LVDASEGPLPQTRFVLKKALEAGLKIIVVVNKIDRKDARPKEVLDEIYSLFFDLDATE
                     EQIDFPVLYAVGREGIAQKSAEEQGRDLTPLFQAILDKIPPPVYDPDEPFQMLVADLD
                     YSDYLGRLAVGKVFHGTVGCNEEMVCLDREEKAIPLRVSKLQVYDGINLKEVESAQPG
                     DIIILAGIEDVAIGDTICTREAPKALPRVSVDEPTISMLFTSNTSPLSGKEGKYVQSG
                     KIRERLFKESLRNVAIRVEDAEETDNFIVKGRGEFQMAILIETMRREGFELMVGRPQV
                     IYKQKSGKTLEPIEHLFIDCDENFIGAVSEKLSRRKGRMVNMVNHGTGRVRMEFTIPS
                     RSLIGYRNEFLTDTKGTGIMNSYLKGYEEYRGSFPLRLTGSLVSDRQGEAVSYALFNL
                     EPRGSLFLTPGEPVYEGMIIGEHNRESDINVNPCKMKKLTNMRASNKDDSIQLTPVIP
                     LTLEKAIEFIKDDEMVEVTPRSIRLRKAFLSAETRHRLRAEKLNA"
     gene            complement(27777..28787)
                     /gene="amrS"
                     /locus_tag="VEL68_18325"
     CDS             complement(27777..28787)
                     /gene="amrS"
                     /locus_tag="VEL68_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXZ37987.1"
                     /translation="MKEAMLYEKLSEQRVRCNLCNHRCLIGEGKKGICAVRENREGTL
                     YTLVYRRLISRNVDPIEKKPMFHFAPGSRSFSIATVGCNFRCDFCQNYEISQMPRDEN
                     QITGQDASPEEIVAMAKKTGCKTIAYTYTEPTIYFEYAFEIAQRASKEGLKNIFVTNG
                     YMTEEALRTLHPHLHGANVDLKAFREEFYQKRCGAKLEGVLKSLKVMRELGVWVEITT
                     LIIPGLNDSEEELRDLAQFIVSLGKEVPWHISRFHPMYKMLDRSPTSVKVLDRARKIG
                     LDAGLRYVYTGNVPGDPGEDTFCFRCGQLLVDRLGFQILKYQIQEKKCFKCGMEMDGM
                     DL"
     gene            complement(28800..29549)
                     /gene="tatC"
                     /locus_tag="VEL68_18330"
     CDS             complement(28800..29549)
                     /gene="tatC"
                     /locus_tag="VEL68_18330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXZ37988.1"
                     /translation="MGWRDKVKDLDRNEVFDKLEGLRGALIRMFLITMALMAVSYFFW
                     KDALHLLQKPLGLPLIMYSLPEAFITSLRLALFMGIFLAAPFIFYGFWRALYPLLASY
                     FPNRYSLVVVLSATALFYGGCFFCYFLVLPVGINFLVTYGSENLEPMIGLAKYLTFSI
                     GLIFAFGLIFELPLIMLILGRAGLIGYQVLAKNRKYALLINSILSAVLTPTPDAYTML
                     LMMIPMQILYELSIWLVKIFGKKKAPEPEPT"
     gene            complement(29701..30921)
                     /gene="glgC"
                     /locus_tag="VEL68_18335"
     CDS             complement(29701..30921)
                     /gene="glgC"
                     /locus_tag="VEL68_18335"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009098308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXZ37989.1"
                     /translation="MDERILAMVLAGGKGERLYPLTQDRAKPAVPFGGIYRIIDFTLS
                     NCLNSRVRRINLLTQYKSLSLNRHIFQGWNIFNPQLNEFINLIPAQQRVGEDWYLGTA
                     DAIYQNIYVLQQDRPDLILILSGDHIYKMNYREMIAHHLEKKADLTAAVIEMDKSLSR
                     QFGVLQVDEEFRIIGFEEKPGVPKTIPGYPALILANMGVYVFNTEILARRVIEDAKDP
                     DSQHDFGKNVIPAMVGQDRVYAYSFRDENKKQAKYWRDIGTLDAYYEASMDLVAVDPQ
                     FNLYDSNWPIRTYYPPLPPAKTVFEEHGSNRVGVSFSSIVSSGCIISGGRVYRSILSP
                     NVRIHSYSEVQDSILMDGVEVGRGARVRRAIIDKRIRIPAGYQIGFNQEEDARKFTVT
                     QSGVVVISKGTILA"
     gene            complement(30934..31314)
                     /locus_tag="VEL68_18340"
     CDS             complement(30934..31314)
                     /locus_tag="VEL68_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ37990.1"
                     /translation="MEKVLCIDDDLSLLRLYQEELAEEGYKVVLARDGKEGLAKFEKE
                     KPQVVVMDIRMPVMDGIETLTAMLGKDRQVPVILNTAYPQYKENFMTWGAEAYVVKSS
                     DLTELKQKIRDILDRRKKMGSQKE"
     gene            complement(31583..31819)
                     /locus_tag="VEL68_18345"
     CDS             complement(31583..31819)
                     /locus_tag="VEL68_18345"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HXZ37991.1"
                     /translation="MNAKLIGIIVLLVLLVVLAIQNYQPLTLKFLFWSLETSLVLVLL
                     VSFLIGALVGGMVLWIGRGKKKDLPPISAEKTEP"
     gene            complement(32099..35170)
                     /gene="treY"
                     /locus_tag="VEL68_18350"
     CDS             complement(32099..35170)
                     /gene="treY"
                     /locus_tag="VEL68_18350"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HXZ37992.1"
                     /translation="MEMKKETAPEPASTLPRLAEKTSLQARIPRATYRVQLGKSFTFR
                     QSLALVPYWDELGISDLYASPFFKPCDVEGAGYDVCDHKEINPAVGTPEDFETLSASL
                     RERSMGLLMDVVPNHMGISRGNPWWMDVLENGPSSVFSCYFDIELRPVKPEMQNKVLL
                     PILEDQYGKVLEEGKFQLKLEEGAFFISYYEHKLPVSPRTYGQVLEHPLEELIRTLGK
                     ENEDLQELRSILTALGYLPSLTESAPEKLEELKREKEVIKRRIASLCQKSPEVRTALE
                     SEIQAFNGQPGDPSSFDRMDSLISAQVYRPAFWRVAADEINYRRFFDINHLAAIRMEL
                     PQVFEEAHRLVFRLLGEGKVTGLRIDHPDGLWNPRHYFLQLQKNYLIHHLRSRLAPQG
                     ETPASRFEEEAEALLGRIRERTPAPPWPLYVVAEKILAEGEPLPGDWAVYGTTGYDFL
                     NQVNGLFVDGGNAGAMDGVYVHFVGVQIPFGSLVNTWKKMIMLVSLASEVAALSHQLE
                     RISEKNRGYRDFTLNSLTFVLREVIACLPVYRTYITGPGNVPERDVAYIEAAVEEAKR
                     RNPRTAESIFDFVRDTLLLRNLQHFSGEDWEGLTQFVMKFQQITGPVMAKGLEDTAFY
                     IYNRLVSLNEVGGHPEKFGGSPDEFHLRNIERSDKWPHSLLATSTHDTKRSEDVRARM
                     HVLSEIPGEWRAAVNRWGRWNASKKTPVDRQPAPDRNEEYLLYQTLVGAWPVEAPEDP
                     LAFDSEEFTQFRERIAAYMRKATLEAKVHTSWINPNDEYDGAVKNFVLNLLEPRGKNR
                     FLSDLQAFQRRVAYFGRWNSLSQVLLKLTCPGVPDIYQGTELWDLSLVDPDNRRPVDF
                     GLRQSLLSGLKERTEKSGENLVPLARELLENSWDGRIKLYLIYRTLQARRRAPKIFSE
                     GSYLPLEGEGEKEDHLCAFARIFKEDSIVVGVPRLVVDLLGGREEPPLGEAAWKDTWV
                     RVPGEWAGRKYRNLFTGEELDVRDGGRGAGFSLGDLFRNFPVALLESMEPS"
     gene            complement(35241..37085)
                     /gene="treZ"
                     /locus_tag="VEL68_18355"
     CDS             complement(35241..37085)
                     /gene="treZ"
                     /locus_tag="VEL68_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXZ37993.1"
                     /translation="MKPEITLGAVYLGGGRSRFTVWAPRARRVEVLLLSPEERVADLE
                     EKDGYHQATLEGVEPGSLYLYSLDGRIERPDPASRFQPKGVHGPSQVVDPAFPWADSC
                     WSGIPLKDYVIYEIHVGTFTAQGTFEAVIAHLDELKDLGITAVELMPVAQFPGDRNWG
                     YDGVYPFAPQDSYGGPAGLKKLINACHQKGLAATLHVVYNHLGPEGNYLRDFGPYFTD
                     RYRTPWGEAVNFDGPQSDEVRRFFIENALSWVTDYHLDALRLDAIHAILDFSAQPFLE
                     DLALVVKEQADHLNRRVHLIAESDLNDTRPIRSRERGGFGLDAQWNDDFHHAVHTLLT
                     GERAGYYRDFGRVEGLAKAWREGFIYSGEYSVHRRRRHGNSSRPIPARRFVVFAQNHD
                     QVGNRMRGERLSFLVPREGLKLAAGAVLLSPFIPLLFMGEEYGETAPFQYFISHSDPD
                     LVEAVRRGRRQEFSAFEWQGETPDPQSEETFRRCKLNHDLRREEGHRILREFYRELIH
                     LRKRLSPLAFLSKEHLEALVLEKEKVLWVRRWRREEKVFMAFNFGQSRVLVNLPVGEG
                     SWRKEIDSEDEKWKGRGSLNPSLIHSQGQASLALHPWSFILFSRTEEV"
     gene            complement(37082..37216)
                     /locus_tag="VEL68_18360"
     CDS             complement(37082..37216)
                     /locus_tag="VEL68_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37994.1"
                     /translation="MAESVRYKVTLLTEDELYLFNQGTYFRLYENLSPVFLKSEGGRR
                     "
     gene            37674..38648
                     /locus_tag="VEL68_18365"
     CDS             37674..38648
                     /locus_tag="VEL68_18365"
                     /inference="COORDINATES: protein motif:HMM:NF025226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXZ37995.1"
                     /translation="MGLKIIPALQIWGALFLGMVAFAAEPSEPATVGVGILPVYAGPS
                     VTSGIVKFLKKEEGIEVQSELFNPEGKWCAIVEPSQKTHLGYVNCEGLIFPGPQKQTS
                     ARGEESRPLASLKPEPIRPLARSTSTESREEKQPAPAFGEFLQALWQEDLARVENMLK
                     AGIDPNTQTTMGNRPLLIAAKKNNPRLLEILIAKGANVDAADLNGLTPLMAAASMGLD
                     QNVRVLISAGAKVNARDEKGSTPLIWAAISGHPQVVEILLAHGADGKVKNKDGHTALS
                     LSKRINASRKRSLAEAEEANRKEELAELRKGLARHEAVLLLLEKPEER"
     gene            38645..38962
                     /locus_tag="VEL68_18370"
     CDS             38645..38962
                     /locus_tag="VEL68_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ37996.1"
                     /translation="MTLSGIQDRHPNFPSGILLHFPKRPKKSFPELAGKAFTALCSRD
                     ADILQETGTDLILASFAHAAREAAETLMAETCIEATYNQASESLVEKNGWITSPKKPG
                     ENP"
     gene            39219..39770
                     /locus_tag="VEL68_18375"
     CDS             39219..39770
                     /locus_tag="VEL68_18375"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXZ37997.1"
                     /translation="MEEDVIMTPVGVLMVEHLLIARMMNLMERKLAGIKPETKPDMVF
                     IDGAIGFAKTYADACHHGKEESIFFDKLAMKNLLPEHKKLMDELVLEHIQTRKIVANL
                     EMAREDFMKGDSGAVGRILIICKSLWQIYPAHLEKEEKAFFPPSLEYFSKREQEEMVK
                     QFWEFDRNLLLERYIKFFDLYDR"
BASE COUNT         9070 a        11097 c        10643 g         9099 t
ORIGIN      
        1 gacatctgtg tcctccgagg tgaggaggat gacttggttc ttcatggtca cgcccaaggc
       61 cgaccgcctg gcgacctgct ttttcatctg gagttcccgt ccattaacca ccagccgggg
      121 gcccacctgt aaagcctggg aaatattcca tccattttgg tactccgtgg tgtggatgat
      181 ccggggcaga tcatcctgaa ccaagaaaat tccccaatca gccttccgca gcgggttggc
      241 ttcctttccg tcaacgatca acaatccgag cggtcgatac ccaggaagga agaacccccc
      301 attgatcccc gccaaggcct gggcttttcg ggccagggcc ttgatctcca tccggtccgc
      361 accatgactc cggctatcca ggatcctgac ctggaattgg tcccaaccga tcctcaggat
      421 tgtcagtttc acgagcgatt ggtagggcag accctccacc cggaagaatt tgtaatcaaa
      481 tccttcgccg accttctgcc aagggaagtc cgatgaggcc aggctatgtc ctccagtccc
      541 cagaagaaag aggaatagaa tcgcccttag gaatgatact ttcattatcg acagtccttc
      601 cctataatga atacattata attccaaatg gatatacaaa gcaatccctg tctcatcttg
      661 cccccccctt cattttccat aaatcgctta aatatcataa acttggaggc cttgcccgac
      721 ccgggttcaa ggtccagcct ctggcgggac aggatcggcg ggggtcagag acccgatgga
      781 aggtcgtttt ccttccgggg ggagggacgg gaaaggcata gccatctgcg cctcacaggc
      841 ccttaccgcc cgtaccgatg aatgaaacca cgcctcatca tgtcccgcca gaggctggac
      901 cttgaacccg ggccaagaaa aaagcgatct taaaagtgaa tgtgggaaag gctcccaatc
      961 catttgactc ccttccggtc cccgattata attgggcttc ctgaatgctg ctgtttgcca
     1021 aagatagaaa tgcccgggaa aggggaaatc agacgctgaa ccctttgatt caaaagggcc
     1081 cggctaagcg gctatcggtc gtgattcctg tattcaacga agccgggacc ataggagaag
     1141 tcatcgaccg ggttcaagcg gccgccctgc ctgaagggct cgaacgagag atcattgtag
     1201 tggatgattt ttccaccgac ggcacgcgcg agattctccg ccaatatggc tcgacggatt
     1261 cctttaggct attcctgcat gaccgtaact tggggaaggg ggctgccctt cgaacgggct
     1321 ttgcccaaac taccggagat atcgttttgg tccaggatgc ggacttggag tatgacccgc
     1381 gggaatatgg caaattgctt gaacccatcc tggaaggcaa agcggacgcg gtatatgggt
     1441 cccggttcat ggggggagat cccaaacgca ctcttctctt ctggcattat ttgggtaacc
     1501 gattcctcac caccctctcc aatctcttca ccaacctgaa tttatccgac atggagacct
     1561 gctttaaagt ttttcggggg gaccttatcc gcaaaatcaa aacccatgag aaccggtttg
     1621 ggtttgaacc ggaggtcacc tccaagatcg ccaaatcggg agcccgggta tatgaagtcg
     1681 ggatcagcta ttcgggtcgc acctatgagg aggggaaaaa gatcacctgg aaggatgggg
     1741 ttcgagccat ctggacaatc ctaagatacc attgaatatc tcctccccct ccgattctca
     1801 tctccctttc ccgtcctcat ccgcaccctt tgttttgctc ggctgcttct ccctcttccg
     1861 ggttcggatg gaaactgccc ctgctcaggt caaacccaaa ggaaggattc cctaaacctc
     1921 ttttttctga taagtttatt tatatggcta gagtaaacgg gaacaaggat tattacagaa
     1981 ttctgggggt atcacgggag gccgcgcccg aggaaatcaa gaaggcttac cgccagctgg
     2041 ccctgaaatt ccacccggac cgaaatccgg gggacgggca agccgaagag cagttcaagg
     2101 agatcagcga ggcctacggg gttctcatgg acccggataa gcggaaacag tacgacctcc
     2161 tctcccggtt ccccttcgaa gccggcaggc ggcctgattt ctcctacact caggaagaga
     2221 tctttcggga tattttcaac aacccccagg cgagcgacgt cttttccgaa ctggggcggg
     2281 agttcgcccg catgggtttc cgtttcgacg aacgtttctt cgaccacctc tttttcgggg
     2341 ggcaggggtt tttcttcatc ctcggcggcc ccggagggtt ccggtattca tcctcgggag
     2401 ggtcgcagga aaagacccgg aacgctgcgg aggtcttctc ccccattctg gaaaatgttc
     2461 tcgtcccccg aggaggtacc ctgacgggaa gaatcgcttc ctgggtcttt cggaaaggga
     2521 tcgggttctt cctaagcaga ctcatgccca atccctcccc gtccggacct aagggagaca
     2581 atttcctgga cctcacctat acccttcctc tttcccgtac ggaggtttcc acggcgacca
     2641 caagggaatt ttcttttcag cggaatggaa aacaggaaaa gctgttagtc aagatccccg
     2701 cgggaatcag ggacggcacc ttgcttcggt tgcgggggag agggaaaaat cagggcggga
     2761 aagtcggtga cctctactta agaatccgcc ttacctaatt ttttttcttg gttgtcctct
     2821 ctcgggaaat ggatggtctt cattctccac cataccctcc tttctcttaa gaaatattca
     2881 gagaagggat ggggcatcta ttctcaatta tctccaatag caagaacaga atcttttcca
     2941 ttggcgcctt tctccattgt aaattcggat catagtttct tcctgaaaac gctaggaacc
     3001 cgctttttga aaaaaaggta gattataggt gaaaattccc agaaagcttt ggcttggctt
     3061 atttctttag gattccagcg gcttgctgaa aaacttcacg gggatgtcat cgcgagtgaa
     3121 gcgaagcaat tccaatcaca taaaagtcaa atacttggag attcccgcgc cgctaacgct
     3181 ccttgcaata accggcagaa ttgagtcttt tcgcaaactg ctagaagttc aattttaact
     3241 ccaaaaagat tacgagggat ttgaagatat cgttctcttt aaacttctct aatgtccagg
     3301 atggagggct ttcttcttta aattgagcca cagacaacct agagatgaaa tccaatgaag
     3361 ggtcaagatt gtttttggtt agatggattt tcccgaaatg atagccggat ttttctttcg
     3421 atggctggct gttctcggaa tggaacagtt ctctttcttg aatttcagtt gcagcaggat
     3481 agtgcatggg atctgtaatt tccggaccca agggatgctc tcggtttttg atggccttgc
     3541 ctggcttagc gccatttaga gtacttccca atcctgcttt tggggacgat gaaaaaggat
     3601 cctgcccttt ccgctgcaat aaccattttc catagatcag ctcatttaaa gagttcgtag
     3661 ggaatatccc ttttgcttct tcgtcagcga agctaaggct atagaaggac agaaccattg
     3721 tgaccagaat gaggctgatc ataaaaaatt tgcccatctc atgtcctcct tttgggagaa
     3781 agccttattt cgtattgatg cgaattgaat gccaaaccta cgcaaataat tatccaataa
     3841 tttcaaaggg taatagcttg gaacccttta tcctggtgac atttttgaac accgaatgga
     3901 ttaatacaag gctgcaaaag aggtaaagta gggtagggta aaagctggat gaggagtact
     3961 tcctcaatac atcgggcgac agagtctggc aaccggtgca ggtttcaatc aataattgtt
     4021 gagaaaggca taataaaaat cccctaacga aattttgtgg gaaatgcgaa gattggcact
     4081 ctctccgcga ccccctctcg ttattgattc tggttgggaa ttctgatatc ttggcatcgt
     4141 cgttgcccta tcatcagatg attaaccttt caacctgact ttatacctcc gggggattca
     4201 tgaagtgggt ttcccagaaa atgcctatag gataggctcc aagggattca gaaattccct
     4261 ttggggcctt ttttatgctc aagacgtgtg ttcttacatg gaatcctaaa acccccggca
     4321 tgatggaaaa tgcaggtgtc caatttccgc cagccgtccc ttcgagggac gtcgaatcgc
     4381 ctggaacagc actcaacgaa aagtaacgag aggaggtcga cggtgaaagc gaaaaagttc
     4441 gtcttgggtg cgatcgccac gatcgtagtg ggatggatag cttttggggc tgacgtgtcg
     4501 gtaggccagg agaagaaaac cagagtctac gagctacgga cgtacacggt gctaccagga
     4561 cggctgccgg cccttcacaa gcggttcgcc gagcacacga tgaagctgtt cgagaagcat
     4621 ggcatacgga acgaaatgta ttgggtgccc atcgatgacg cgcgcaagga caacacgctt
     4681 atctatgttg tgtctcatga aagcatggaa gcagcggacc gaagctggaa ggccttctat
     4741 gccgatcctg aatggatcaa ggtgcgcgac gccagcgagg ctgacggcaa gatcttggcg
     4801 aaaccgcccg agcgtgtatt catgaagctg accgattatt ctccatcgcg ttaggtgctt
     4861 tgaagaggtt gcacggtata aaggcagttg atgtcggtcc cccacaatat aaaaggcagg
     4921 gccatttctg accttgcctt tttgtctccc aacctacaaa ggttcaggca tttaggcgaa
     4981 aagttctact cactgctttc agaccatcta agaagttttc gaatatgatc ttcatacact
     5041 caattgggag atttgatttc tgcccctaac cattgatctc cagatttatt ttaaggtgaa
     5101 ggcgagaatc ggatcggggc ctaatttgag gctgtaagcc cgactaaata ttcgtcggga
     5161 gatttaattg ttcaggctca tgagcggggc ggggatccgg ccgccgaagc ggacaaaatt
     5221 gcccgagcgg ttgatgttcc tcacctcgag gatgtaggct tctccaaaaa gccctccaaa
     5281 gaccgcctta tctcctacat ccttccccgg aaccggaatt aaacggaccc ccgtagtttt
     5341 atgattgatc actccgatgg ccatttcatc ggccaggagt gcggcaatcg tgtccgcatc
     5401 cacctttccc ggaagggcga tcatgtccag gccgacggag cagacggcgg tcatagcctc
     5461 cagtttctcc agggtgaggt aaccctgctg gacggcccgg gagatattca agtcttcgct
     5521 cactgggatg aaggccccgg acaacccgcc caccgaggag gaggcaaagg ctcctccttt
     5581 tttcaccgca tcattcaaca gggcaagggc tgcggttgag ccagggaccc cgatggattc
     5641 gaggcccagg cattgaagga tctcccccac gctatctccc accgttggag tgggagccag
     5701 cgataggtct acgatgccga agctgacgtc catctctcgg gctacttccc gggcgatgag
     5761 ctctcctacc cgggtcactc taaagctggt cttcttgatg atctccgaca ggtcccccag
     5821 ggtgagcttt ttgcccgatt gaaccgcccg ttccagttct ctcttcacca ctcccggtcc
     5881 actaaccccc acattaatca cacactctgg ctcaccatac cccaggtaag ccccggccat
     5941 gaaggggttg tcctcgggca tattggcaaa gaccactagc ttggcacagc cgaacccgtc
     6001 gcggtcggct gtgcgctggg aagtttcctg gatcgcttgt cccatgaggg atacggcatc
     6061 catgttaatc cccgcacggg tggaggccac gttcaccgag gcgcacaccc tttccgtact
     6121 ggagaggact tcgggaatcg tatccagcag ggttcggtct cccctcgtaa agcctttctg
     6181 caccaacgcc gaatacccgc cgatgaaatt gacctccact tcttgagcgg cctgatccag
     6241 ggtccgggcg accatcaaaa aatcctcgcc cctgaggctt tcagccaccg acgcgatggg
     6301 agtgaccgcc agccgtttgt ttacgatggg aatcccgtac ttggtggcga cccgttcaca
     6361 ggtgggaact aggttccgag catgtctccg gaccttctcc cggacccgac cgcagacttc
     6421 gtccgcggaa gggctggcgc agtctcttag gctaattccc agggtaactg ttcggacatc
     6481 caggttttcg ttcttcagca tctccacggt gcggatgatt tcttcggaac tgagcatata
     6541 aacctcttgt ttcgggtttc aagtttcagg ttttgggtta agtagccgtt tcaactcgaa
     6601 acccgaaacc ccaaactcga aactgatttt tcagatttga ttgatggccc gaaaaatatc
     6661 tttgtgctgc aagtccacgg aaacgcccat ctcttgcccc cgctggtgga tcttttcctg
     6721 aagaaggccg tagtctaagt ttttgggaat atctacttcg aagacctgga tgtattcgag
     6781 ttcctgcccc agcctggcta cttgagcacc caggttggta atgttgatcc cccgttcagc
     6841 cagcaactcg gtgatgccat agaccagacc tggccgatcc ttaccgcgtg cggttatgat
     6901 gaaaggttcg gtctccccgg attggtaggg aatcaggtcc tccggtttga gccgccgtag
     6961 gtcgatatcc aggtccaggt ccctggacag gtcacggagt tcgcccttta gggccgggac
     7021 cgatgctcct tctcccagag aggcgatcag aatcatggcg aactgtcccc ggatgacagt
     7081 ttgagagagc tcttcaatac tggagcgatg gcggtagagg actcccgaaa cgttggccac
     7141 gatccccggc cggtcccggc caatgaccgt aatggccacc cattccattc gaggatcatc
     7201 attcatcctt tcgccccccc tttcctttat cagctcctcc ccaattttat tcctatgctc
     7261 tctcgaccat ccgaatccgg aatttatcca caagatgatc cgggaagtag gactccttcg
     7321 ccttgcgaag tccgggttcc cccaagtcct gttctcggtt gatccaaaga acacgggacc
     7381 aggatttttc gcagaactgc tggttgatca tgggatagag tccgggaatt tctgggtttg
     7441 ccttttccag gtggatgacc gctgtctccc ggttcagcaa ctcagccagg gagaaggctt
     7501 gcactttatc ctggatcaga agaactcctc cttgaatccg taatttcgtg aaatgggtaa
     7561 gggcttcccg taccgcctcc cactccccca tcaggttcat atccccctca cacctgcgaa
     7621 cctcgcacca gaatccccca agttccaggc attccggcaa atggcgttct tccaggggag
     7681 agtaggtaaa ggcataggtc tggaggaatt tattgatgtg gtttcgtttc ccgtggtatt
     7741 tacgcccggc cagctggatg aggtcttggc ttcggtagag gtagtcgaat tgttcacgag
     7801 tgggttcggc gaccaggtcc gggctatcct gaatctcatc caccaaacgg ctgtcggctc
     7861 gttcgatgcg ggggttcttc ttccccttct cttccttaag ccatttcaga gattcttgga
     7921 ccacctccag gcgcgaagga gggccgatgg gggggagaaa aaaggctcct tggcgaccag
     7981 cggtactcag gaccagcagc caatcccgat acagggacca ctggaacccg taataggacc
     8041 gccagatgaa gaggttggaa aaagtccact ccgaggtctg gggttgatat ctccagatga
     8101 tctcccctat gacctcgcga tcttccagtt gaatgagtcg gaactgggga aattccggga
     8161 tcatcatggt ttctgcttgc tcgttcccgg ttgctagttg gcctttactc gttgctggtt
     8221 actggtccct cgtcaatggc tacttaggct tgggtgagtc agggtgaatg aaaaggggtc
     8281 gtaactggaa acaaggaacg agtagccggc aacgattctc aactattttc cagcgctgca
     8341 ctgagacgct tcaggtgatt cctctcttgg ttggacaggg ccagaaaaac ttctttggcc
     8401 tctgttccct ctattctgcg ttccatcttg atgtagaggt cgatggcgtt ggcctcgagg
     8461 gagatggaga actccaggat atctttcagt tcttttcccc gggaccactc caggacttcc
     8521 ttcaacccca ttcccccctc cagataatcg tcgccggttt ctagggaaac cagcgaacgg
     8581 ggaaaacccg ggtctgaagc tttccgggag attctctggt agattttgaa gagggaagac
     8641 ttgtgtttct cttcatcaac cgatagttct tggaaaaggt caaccgcccc cggaatcttc
     8701 tcctttgtgc ccatctcttt gtaaaacttg cgggacccct cttccagcat ccaggccaag
     8761 gcgatcaact cttccggttt tctggccggc tcaaagtagg ccatcccggc atctggagca
     8821 ccggttgccc gcaggccttt ccaggcatgg atgcccccct ccatgctgta cacttcttcg
     8881 aagccctccc gcgcgagaac tgcggcagcg gcccggctgc gcaccccggc tgctcagtag
     8941 gtaatcgtgg gtttgttccg gtccagctcc ttccaacggg tttccaattc tgccacggga
     9001 atcaggcgcg ccccggggag atgctcctgt tcgtactctt taggggtccg aacatccacc
     9061 aggttgtact cctgcgggct cttctcatcc agaaactgtc ggacccgatc cggcggccaa
     9121 gtggacaccg gtttgaaaaa atccaaaatt cccatctttc acctccgtaa cgagaaacca
     9181 ggaacaatca accaggaacg gtttttttac tccgtttcat ccctttcccc tgacacctga
     9241 aatttttcca tgaactttcg atcgatgcaa tccttcagca aaaaagccgt tcgcccttcc
     9301 cacacaaaat tcttcttccc cagaatcccc cgcccgttgc ccataattaa taaccggttt
     9361 taccgggacg atattttccc gggaggcgga gaccagaaat ttcaggggta cctcactccc
     9421 tgtattgaag gatgcgacct cataatcgat ttgtttgccc gatcggagat tcagggttct
     9481 tcggttcgaa ttgatcctga tcactcggtc ttcgacaaaa atacccccac ggtcttccat
     9541 cattttcttt ccgtggaaac gaacttcccg cggctggtag attcccgaga gcatccccgg
     9601 tcccataccg gaataattat agtagggagt aggcccgatc agagtgactc gatgcccgcg
     9661 ctggacgaaa tgggaggtgt taaaaagaac cgtgaggtga gcatgcccac ccccaactaa
     9721 tacaagatgc ttgcccatct ctcccttgtt cggcctacac acccgactta tcttcgggtc
     9781 tgctgattct tgggggcaat gcccaggtca aattttcatt tgtctaccag tcgagattta
     9841 actgaaggtt gactccctga ggacgggaaa attttacctc ctgccccatg aaggaccttt
     9901 cctcagaagg gtctacgccc acttgccgcg caaggacggg agaatggaat tgtactcgaa
     9961 tgtccgattg gatcccaaac aaccaataga atacgctgtt caatgcccca aggggatcgg
    10021 tcagaattaa acctgcatga tcataccatt tcagctcggg tttggccttg atcacatctc
    10081 ggatgggttc gaagacatat ttccccacaa tgtaacctcc cacgggggtg acaatcaggt
    10141 cattcttgga ggggggttcg aaaaaggcct cgattccgaa ttcataacac gcagaaagca
    10201 aagccgagta ccagaaggaa ccaaattctc cgaacccacg ttcccgagcc cgcgtatagt
    10261 aggtagcccc aaaatagggg tgggcaatgt agttgatcca aaatttatca tgatcccaga
    10321 ccggattttg aacgtttacc ttccattttg caaaggttgc ttttttctga tcttcggtcc
    10381 aagaagtcac actttccggt agaaaccaga gaactccggc gaaaaccact tggtaaccca
    10441 gaaaaaaagc ggtatcgatt ccaatccctt tccaatctcg ctgaggtttg ctggactctt
    10501 tctggctcga ttcggataaa ggtgcccccc cttcatctac caaggtatac ctgtgctcgc
    10561 tctttcccaa agaagcttgg gcgaaaaaga gtggttgact cgggtcttcg gcagatctca
    10621 gccaactgaa aatttgcgca ccgaagaagg cgggtttatt ccactctaaa tagacattct
    10681 ggaagaaatc tggcccataa gcctcgcttt ccgttgggtc tatttcccag cgatgatctt
    10741 caacttcttt caccctcccc ggccatgcat cggagcccat ggccacccga aaaccaccca
    10801 tctccagcag cccccaaagg aagaagagga gaagaacatg aactttcaaa agtttttcct
    10861 ccggctggaa gcagtccaaa aacaaaaaag atttaaaatt ctaacatgga ttgcttatga
    10921 aaaggcaatt tctttctagg ccttattttg ccgatcattt ccctggacaa gacaaaagct
    10981 agttgataat attttctcag aaaattttaa taattaggat aaaccgatgc ataagggtgt
    11041 tccattttgt gttggtgtgg caataggctg gggatttctc tttctttctc cctatcttca
    11101 cgcggttgaa atgacccctt tctacactca gaatcagagt cctttggttc agattttcgg
    11161 tttgccctcc atcggtgagg cctccgtagt ccccagccaa aaggccgatc ttaggttgat
    11221 cgtagactta gccaataatt ttgtgaacga taagacctca aacgaaagca tcgtcctgga
    11281 tggacaaagc acccggttca gcctggatgc ccgttatgga atcgccaaga attttgaagt
    11341 cgggctggtg attccctaca taatcgaggg gggcggcttt acggatggct ttattgactg
    11401 gtatcacaat accttcgggt ttcctgcggg aggccgagac cacgccccga agaatcgctt
    11461 gctttaccag taccaaaaag accagcaggt gcgcctcaac gtcgatcagt ccagcagtgg
    11521 gatcggagat atccaattga cgggagcctt ccaactttat cgaaatcccg ataaaccctc
    11581 ccaggccata tccctccgga cctccctcaa gctgcctacc ggagatagcc aacaactcca
    11641 tggaagcggg agtgccgatc tttctctatg ggcgaccgcc ggagacgact ataaaatatc
    11701 cgtcggccat ttcacccttt acggggcggc ggggatcatg ggtatgacca atggggatgt
    11761 cctgaaagag cagcaacgga attgggtggg atttggatgc ttggggggtg ggtggagtcc
    11821 ggcccgatgg attgccttta agctccaggc caacgggcat acctcctttt ataaagacag
    11881 tgagctccgc gaacttaaca tgaattccat ccaattgacg atcggcggga ccctggcttt
    11941 ctccgagaga atctccttgg atgttggggt tacggaagac gtggtcaccc tgacctctcc
    12001 cgacgtggtc tttcatcttg ccctgcgtgg aaccttttga aacaatgtga ataggggcat
    12061 taattaagaa ccacagtcat cgccgcaagg agagcaaaca gcaagtcgct caaggttcag
    12121 acgagaaatc tttgtcttgg aaacggatga agcagaaggg aattccgccc gcagcctctt
    12181 taccctgttc ccttaccttg ccccttgatt caacccttcc ctcgggcttt tttcacctcg
    12241 cccaggaacc tttgggcgtt ttcggcgatc accgagaatt ttttctcggc caccgctttt
    12301 ttatccacca attctccgcc ggcggcgatg gccgcggctc cggccttgat gaactccccg
    12361 gcattctgaa gattcacccc tcctgtgggg accaggggaa tgtggggaag agggcctttg
    12421 agggcccgga tgtaggccgg gccccccacc tgagcgatgg gaaaaacttt gacctggtcg
    12481 gaccccgcct cccaggcggt caggatctcg gtgggggtca gcgctccggg gatgaccacc
    12541 gcgctgtagc gtttgcacat cttgatcaca tccaggttca gggttggagt gacgatgaat
    12601 ttggctccgc tgaggagggc cgcccgggcc gtctccggat cgaggactgt tccggccccc
    12661 aatagaacct ctttgccgaa tttctgggtg acctctttca tcacgtccag ggcaccggga
    12721 accgaaaacg ttatttcaat aaccgaaatt ccgccgtcgc gaatcgcctt ggccacctcg
    12781 aaggcttccc cagccgacgc cacccggatg accgggacca ctccttcatc gactattttg
    12841 gacagcactt cattcataga acctcccact caaatgcgga atgaaaaaaa ttccgcattc
    12901 cgcatttatc ttgaaattct gagtcctgcc cccttgatct gtgcttccac ttcttccagg
    12961 gtggaccagt tgaaatcgcc cggcatgctg tgtttcagcg ccgaaagggc atttccgtat
    13021 tttacgcctt tttcggctcc accggtaagg tagccgtaga tgaacccggc ggtaaaagaa
    13081 tcacccgccc ccacccggtc cacgatttca accgcgtagg ttcggtcaga gtagaccttc
    13141 ccgtcggcgt aggcgatggc cgtccagttg ttcttccaga cggacaagtc ctccctcagg
    13201 gtgatggcca cggctttgaa tccgaaggtc cgggacaggg tctcggccgc tttctgataa
    13261 ttttgctccc ggatcccaaa aaccaccccc gtgtcctctt ccgtggtggt gatgatgtcc
    13321 accagcttca tcagcggctc ctggcatttc ttggcgtcag cgggagacca gagctttttc
    13381 cggtagttca ggtcatagct gactaggcat cccgcttctt tggccgcctt catggccgtg
    13441 gccgtagtct cggcggccga ggggctcagt gccggggtga ttccgctcac gtggaagagt
    13501 ctggccttgg aaagaatctt cttccagtcc acttcgttgg actggatcag gctgatggcg
    13561 gaattggccc ggtcataaag aaccgagctg gcccgcggcg cagccccata ctccacgaaa
    13621 taaagaccct ggcgtccctg atcgctgaaa accacgtggg aagtatctac gcccaactct
    13681 cggccgcggt tgcggaccat cttgcccagg gcgttattgg ggagcttgga aacccaggcc
    13741 gatgccattc ccagccgggc cacgccaacc gctacgttta attcgctccc gccaatttcc
    13801 acatccaaag aatgggtctg ctccagcctg gcaaaatgcg gcggcgacag ccgcagcatg
    13861 gcttcaccga aagttaccac atcgtacatc atggcagcac ctctcattaa cgaaataata
    13921 gtgatgttaa ctgaaaaatc ccttctaggc aatagcgcat gaaacagttc ggagagtata
    13981 ggggcaccca cggcatgccc gggggaggct tattcctaaa ccttctccga atcctgcaat
    14041 ccgcaatttt aaaattcttg tctagaaata actcactagc tctgcttact ctccttcatc
    14101 agatgacacg ccacccggtg gccttctgcc gccctccgca gctctggata ttcctttcgg
    14161 caccgttcaa aggcacaggt gcagcgggtg tgaaaatagc agccattggg gggatggatg
    14221 atgctgggga tatccccctt aaggatgatc cgtttttgga gcccttcggc attagggtcc
    14281 ggagtcggaa tggcggacat gaaggcttgg gtgtagggat gaaggggatt ctgatagaga
    14341 tcatccctcc ctgccacttc tacgatccgg cccaggtaca tgaccgccac ccggtcgcaa
    14401 acgtgctcca ctacgctgag atcatgggat atgaaaagga gagtgagatg gaactctttc
    14461 tggaggtgtt tcagaaggtt cagaatctgg gcgcggatgg acacatccag ggcggatacc
    14521 ggctcgtcgc agatgatcat cttgggataa tccgtgaccg tgcgtcccac cagagcccgg
    14581 gcgatgccga ttctctggcg ctggcccccg ctgaattcgt ggggataccg ttccagatgg
    14641 tccgtattca gcccgatcct ggcgatgatt tccgcgacca cctccttgat ccggctggcg
    14701 ggaacgagat ggaaccgtct cagcggctca cccacgatcc tttgaatcgt catcctgggg
    14761 ttgagggagc tgtaggggtc ctggaagatg atctgcatgc gcttccaaag ctccttgagt
    14821 tcctgcttct taagggcaaa aacatcaagg ccgtcataaa ctgcccggcc ggcatcggcc
    14881 tggtagaggc ggatgagggt tttgccgatg gtggtcttgc cgcagccgct ttctccaacc
    14941 agccccaggg cttccccccc cttgatttca aggtccacgc cgtccaccgc ccggacgtga
    15001 ccgacaacgc tggagaggac acctcggcga atgggaaaga acttttttaa gccttcaact
    15061 tttagaattg tctcgccgtt tatgtccaat agatgcgtcc gcctgcaggg tcggttcgcg
    15121 aaccacccca cttcaatgtt acaaaagaat ggtttttccg aattccgcca tggactacgt
    15181 tgggtttaag gcagccttga tccctgcttg gatcagggcg cattcccgat tccgcattcg
    15241 ttcaaccagc accggctgaa atggtcccgt cccggctcaa aaagatgagg ttcctccatg
    15301 cggcagcggt ccattgcctc cgggcagcgg ttggcgaact tgcatcctgc ggggaggttc
    15361 aggagattgg gaacactccc cggaatttcc ggcagggtgt ctaccctgcg cccgatccgc
    15421 gggatggact gaaataggcc gcgggtatag ggatggagcg gagagcggaa gacctccccc
    15481 accccggctg actccacgat ccgccccgca tacatgacga tcaccctctt caccatgttc
    15541 gccaccaccc ccaggtcatg gctgatgagg atgagggaca tgcccatacg cgagcgcagc
    15601 ttcaggatca ggtccaggat ctgcgcctga atggttacgt ctagggcggt caccggctcg
    15661 tcggcgatga gaatttccgg gttgcaggcc atggccatgg caatgaccgc ccgctgccgc
    15721 atgcccccgc ttagctggtg gggatagtcc ttgatgcggc ttgcgggcga aggcattccc
    15781 acctgatcaa gaaggtgggc ggctttctcg agcgcttccc gtttacccag cccctggtgg
    15841 aagcggaaca cctcccccag ctgatccccg atggtgaacg ccgggttgag agcggtcatg
    15901 ggttcctgat aaaccatggc gatcttttca ccccggattt tttccatctc cttttccggt
    15961 agaccagtta attcctttcc ccggaaaaga atggagccct cagcgatccg gcccgcgggt
    16021 tcgggaagga tccgcatgat ggaaagggcg gtcacgcttt tcccgcaccc ggactctcct
    16081 accaggccca gcgtttctcc aggggcgaga tcgaagctga tcccgtccac ggcccgcacg
    16141 atcccgctca gggtgaaaaa gtaggtccgc aaatctcgaa tccggagtag cgtttccaag
    16201 gttcttatct cttaaaaatt tttttacaaa tatttctttt tagccgtgtt gatccgcgct
    16261 ccacccgcgc cccctgaata attttgtctt taagcttttt ccgcattaaa cttaacgttc
    16321 atctgcaatt atctgtgtcc catcaggttt ttactctttg aaccgggggt ccaagacgtc
    16381 ccgcagccca tccccgaaaa gattcagcga aagaaccgca agggcgatgg ccgcccccgg
    16441 gaaataagtc agccagggcg cccggctcag gacttctttc ccatccgcca gaaggcttcc
    16501 ccagctgggg gaaggcgggg gagctcccaa tcctaggaag gaaagagcgg cctcccagag
    16561 aatcgcgtag ccgaaggaga tcgttgcctg gatgatcacc ggggcccagc agttaggcaa
    16621 aatgtggaca aagagaatgt gaatgtcgct gcacccggtc acccgggccg cttcaatgta
    16681 ctctttctcc cggatggaga gaaccgagct tcgaaccagc cgcgcaaagc gcggcgtgta
    16741 gaccaccccc agggcgatca tcaggttggc aaacccggct cccaggacgg ccatgatgcc
    16801 gatggccaga agaatgatcg gaaaggagag gatcgcgtcc atgattcgca tgaccactgt
    16861 gtcaatccac ccccggtaat agccgctgat caatcccagg aggatgcccg cggtcatggc
    16921 cacgatgatg gagaggaacc ctacccgcag ggcaatggcc gccccgtaaa ccacccggct
    16981 gaaaaggtcg cgcccgtagc cgtcagtgcc gaagacatgg gatgagctgg gcggctgcag
    17041 ccgctctttg atgttcaatt taaaaggatt gtgggtggcc acccacgggg cgagtaccgc
    17101 catcgccact aagaagagga gaagcaccgc cccgaggacc gctagtttgt tccggatcag
    17161 gcgcctgaga aacatttccc tcagccctcg aagacgatgc ggggatcaaa ataggagtag
    17221 aggaggtcca cgatcaggtt gatgatcacg aagccgaagg cggtgagcag gatgcaaccc
    17281 tggatgatag ggtaatcccg ctggccgatg gccgtgtaga tgagctggcc gatgccggga
    17341 aggctgaaga tggtctcggt gatcaccgcc cctcccagga tattccccag ctggaagccg
    17401 atcaccgtta gagtaggagc gaaggcattc ttgagggcgt gcttgcccac tacccagact
    17461 ttgggcaatc ctttgccacg ggccgtagtc atgtactcct gccgaagcac ctccagcatg
    17521 gtggagcggg tcatgcgggc gatgatggct gaggccagga gggcctggga aatgctgggc
    17581 agaatgaggt agtgcatccc cttgagaaaa ttctcgaagg gggaaacgta acccgtcgcc
    17641 ggaaaccagc ccagcttgac cgagaagaag atgaccagca tcagagccag ccagaagccg
    17701 ggaatggaga ttcccatgac cgccagaagg gtgaataggt agtctttgta gttgttctgg
    17761 ttgaccgagg ccatgatccc cgagggtacg gcaagaagaa gggcaaagac catggagagg
    17821 aatcccaggg tgagggtcac cggcagccgc tgcaggatga gctggatgac atccaggtgg
    17881 tagaagatcg aggcgccgaa atccccgcgg atgatccggg agagccagat gaaaaactgg
    17941 acatagatgg gctggtcgag ccccatttcc cggcggatgg attcgaccac cccgggctcc
    18001 gcgttggtgc ccacgtagat tgccgccgga tctcccggaa tcatgcgcag gaggaagaag
    18061 acgatcccag caatgaggaa gaggaccggg atcacctgca agaggcgctt gattacatag
    18121 gtcttcatta tccttgctta agccgtcata ggggcaatta atgaattgcc cgtaccgaat
    18181 gctcctcttt cggggtccga atcggctggc ggccacccca tgaaaaaaag ctcatttatc
    18241 cagccacaca tgccagaggc gggcgtcggc cgtgtagggc atgggcttga accccttgac
    18301 atactttcgg gtcgcgggag caaccgagtg gtcataaagc ttgaggagcg gaacgtcctc
    18361 tagcatgatc tcatgaatct gttcataaag cttcttgcgg gtggcaaaat cgctggtggc
    18421 ctcggccttt tccaccaggg cgtcgacctt ctcgttcttg tatcccacga agttccgctt
    18481 ggagtggacc cgcaccatgt ttaggcaggg gtccgtcatg ccggaggcga tgaaggaaag
    18541 gatatcgtgt ttccccgcgt tgtaatcctg cagcagggcc ggccattcct tcacatccag
    18601 ttgcaccttg atccccgctt ccccgagcat ctgctccgcc aggatggcgg ttttgtaggt
    18661 attcagataa accttggtca ccgctatttt gaccggcgtg ccgtcgtatc ccactttttt
    18721 cagcaactcc tttgctttct tcaggtcttt cccgtatccc tttttatgca cgtcggagta
    18781 aaagggatcg accacattga ccagcgacgg gttgaccagg gaatagccga acaccgaccc
    18841 gtccaccagc tcttggaggt ccaggcagtg gcccacggct cggcggaaat cggccatgcg
    18901 gatgaattta ttagtgctgg tgttgacccc gaacatgaac atcacgtagg caaggcccgg
    18961 gctcacgtag gttacgatgt tggggtcttt cttgaactca tcgatctcct cccaggccac
    19021 gcggagggtg aagtcgatat cgccgctctt caaggccatg gaccgcactg ccgcgtcctt
    19081 gatgggaaca aacttaatcc ggtcggcata ggcgggcttc attccacccg ttccggaggt
    19141 gggcaagttc gatccctggt agtctttgtt ccgctcgatg atcacatgct tatcgggaat
    19201 atactccacg aatttatagg gcccagtacc gaccggccgg gtcagggtgc cgccttgttt
    19261 ttccacttct tctttgggaa cgatggcatg agttccatag ggattggaca tgtacaccat
    19321 taaggtgatc gtcggggcct tcagcgtgaa ctggacagta tatttgtcga ccaccttgat
    19381 gtctttgatg ttctggacct gggtcacgaa gggatatttc gtcttggggt ccatgagccg
    19441 actgtaggag taagccacat catcggcgtt catctcgcgc ccgttgtgaa acttcacccc
    19501 ctgacgcagg tggaaggtgt aaaccagccc gtcggggctg actgaccact tggaggctag
    19561 gccgggcatg ggcatgtttt tttcgtcgag gtcgaccagt ctttcataaa tgttgttgag
    19621 cacgatcatc gatatctctc cggtcacttt atggggatcg atccccacga tatccgtgac
    19681 cgtccccacc accagcgtgc caccgtattt gggctgctgg gcgaaggata agcctcctac
    19741 gatcatagcc ccgataaaaa caacccaaag aacgccccat ttttcaaacc gaatcatgag
    19801 cgaacctcct tttcgagttc cagccgatca atcgattcat ttgtctcccc tccatcggtt
    19861 attcatcatt gcaggagcca ccttacaaaa aagggaagac tctagcgctt taaaaaggcc
    19921 ttcccagaaa tccctccccc ctttgggtcg ctctgtaaag ttatcttagg gcgagacccc
    19981 aaatttaggt cttcccttcg aagtaaaata acaaattaaa ttcacacaac ctacgatagc
    20041 gaggccaatc aaggtggtat aaatttccgg gaaggcgatt aatagacccc cggtaatcag
    20101 ggaaatacgg atgggccaat tgatttggcc gattccccag agatagcctt ccaaagcgga
    20161 gcccaagtaa taaatgccaa ccaccgcaaa gaaggtgact agcaaaacct cccaccagac
    20221 tccgtgagtc aacaaagcgg ggttataggc aaagaagaaa ggaaggatgt attttaccgc
    20281 cccgagcctc atggcggccc atcccgtctg cataaaattc gagccggcca gtccacaggc
    20341 ggcgctgaca cagagagcta ccggcggagt tatctcggat aaaacccccc agtaaaatac
    20401 gaacaagtgg gcggccagag gatcaatccc cagggagatg agtgcgggca ccatgacgat
    20461 ggccaggaaa atgtaacagg ccgtgatggt catacccaat cccaatacaa aactggaaaa
    20521 ggcccccatc agaagaatca ggaaaatatt atctcccacc gccccgacta gctcacggga
    20581 aaaggccaaa gccacaccgg taaccgaaag gccgccgatg atgagcccgg cagcggccaa
    20641 gatggccacc agctgggtca cgactttccc gatattcgct atcatggcta tgaacttctt
    20701 ccaatctagg cggctttcct ttttgatcat ggataaggcg actaaagcag cgcttgcata
    20761 gtaaggggcc ctggcttcca atcgcaaggt cagcagaaaa tagaccaaca atacaatggc
    20821 taaagtatag aaccagccct ctttcagggt cccccagaag gagggtagtt cacccttagg
    20881 taagcccttc agattggctt tggccgcata cccatccacc tgaacaaaca ggcccaaata
    20941 gtacaggcag gcgggaataa acgccccgat tgcgaccacc gcgtaaggga cattcaagaa
    21001 agaggccatg ataaaagctg ccgctcccat caccggaggc atgatcggcc cccccgtaga
    21061 agcgcaggct tcaatggccg cagctaagtg gggcggaaaa ccggtgcttt tcatggccga
    21121 gatagtcatc gccccgatcg ttagcgtatt ggaagtcgtg ctcccgctga tcatgccgaa
    21181 aaaggcactt gccaaaatgc tgatcttggc cgctcccccg cgagtatggc ccagcaggga
    21241 ctgggccagc ctgaagaaga aggtgcctcc gccggtattc tgtaggacga cgccgaatac
    21301 catgaatccg atcagcagcg tacagactac atctaggggg attcccagaa taccgctcga
    21361 acccatggca tacagccgag ctgaagtcaa aaagtcgtgg gactgcccct gcaaaaaccc
    21421 gggaagataa acggcaaata gaggataggc ggaaaatatc aggcacataa gggtcatata
    21481 gatatccgtc gatcgcctga cggcttccat gaccagaacc cataaaagta cgctaaaaat
    21541 ggtcgccggc aggggagatc gatattccca gccccgttca ataatgtcga ttccatgaat
    21601 cccgaaatat atattgatta ttaccacgac cgagaagaga accacgtcat accagggcac
    21661 cttgtctcca gcggattttg ccgcaggata aagaagaaag gcggttgaaa ggtaaagggt
    21721 aagattataa taaagataag aggtgtccag gggattgaaa ccgaacaact ccaagcggaa
    21781 aagctgattt acggccagca atatgcccag cgaagaaaag acgacgaaaa taacgtgcca
    21841 aaactggcca atcttctcaa accggctgcc cataaaattc agtatcccct tcgatagcta
    21901 ggcttccacc aagaaaatcc caaacgctgc aaaaaaacac caagaacatc acccccaccc
    21961 tccccccctc taagggagga aggtcagggt ggggggtcga agaatccctt ttgttaaccc
    22021 aagaaaatgc tattctgtaa tttcacctct cttcttcaac cagaattcag tgaagtcctt
    22081 ttcaggcatt cctttcgcgg tcgcttcttg catggcggtt ttccaggctc cctggacttt
    22141 ctttaagcgg tccaaaaact tcctattcca ttggtcggat tgtgcagtcc agattccctt
    22201 ttctttaagg taccgaatcg ctccttcatg aaagggcgcc ccagccggga ctcttccaga
    22261 ccttgcaata gcccattcag gcagctctgg gtcggcatct ttatacatat cgaaggtttc
    22321 atcaaagcct tttaccaggg catagacttc attggcatcc tgtttggcat agcagaccag
    22381 ctggggatag gtataagagg gaatctcttt gggctggccc tttttaaggc ccggacctat
    22441 atcgtatttt cctgggctca gccaaggggt tagttggtgg agccttttcc acccttctac
    22501 atcggatgcc gggaactccg gccaggagat cccctgggga gaagcttcca gttcatacag
    22561 taaaggggcg gtgatcccgt aactgatggc atcgattttc ccttcgatca aacctttccc
    22621 cgcggccgca tagctgggca ggtcaattct ctggacatcc ttccaggtca gtccagcgaa
    22681 ggccagaaag gcttcggtct ttacattgtt cgtggaagcc cccgggatcc accctatctt
    22741 tttccccttg caatccttaa gggtcttgat tccgctctta gcggtagctg ccatgcctat
    22801 gggcgcaggg tgggccaata aaactctcaa atcctgcggt ccccagttcg gaaaagagaa
    22861 ctcataaagc gcttcagctg caaaataggt ctcatccgcc agcagcccgt aagtggcggc
    22921 ccctgtcttt atgggaatga gccttccaac cgatgtgccc gaaggtagaa ttctgacctt
    22981 ggttgcatat ttcttcgtta gagcattgga tatggctgcg ccttgcacat agccccgcga
    23041 acccacgtca aaggcagtcc aggtcatcat tcccggcaaa ccggaacctt tttcggcagc
    23101 cggcgatagg ccggccacaa acaacaagcc ccccagaaag agccctaaaa cgacccacct
    23161 tgcctttgcc ttcttcatct tgccgacctc ctctcttctt ttctaggaaa tcttctttat
    23221 cgataggtct gaggaagaaa tctcccttca ctcctcgatc cctatgtttt ccggggatta
    23281 ttcgcatgcc tcattttttc cctatcccga gcatctggcg aacttcgtcg ggttgggcaa
    23341 tgtctcgacc caactctcgg gccagtcgga ccaccttctc caccaactcg gcattacttt
    23401 tggccaggac ccccttggag aggtagacgt tgtcttcaaa gcccacccgc gcatgccccc
    23461 cattcaggat ggagagggta accgtggaga actcgtgccg gccaatgccc agggtcgtcc
    23521 agacgacatc tctcgtcggc aggaggtctt tcatgaacaa gaactgccgt atcccggcat
    23581 ccatccctcc ttccaccccc aggcaaatct ggaataaata aggaggatcg accaccccct
    23641 tttcgatcag gctcacggca atacggacgt ggccggcatc gaaacattcc agctctggtt
    23701 tcactcccct ttcccgggcc actttggccc cgatcggccc ccattccggg gggttgatga
    23761 agacacggtt ctggtaattc atgctgccaa catccagggt gcagagctcc gggccaaggg
    23821 tcgtggtccc gaggcgtctt tcgatgaggt tgtcgcccgt caggttggcg gcactggtcg
    23881 tgaaattgag caggatgttg cagcgggaac ggacccggtc gcgaacctcc cggaaataat
    23941 cgagctcgaa tgaaggggct cctgtcttgg ggtcccgcac gtggacgtgg gcgatggagg
    24001 cccccgcctc gaaggagcgt acgatctcgt tggcaatctc ctcgggggta tagggaatgt
    24061 tgggattatt ttttcgggta ggccctgacc ctgtcacggc cgcagtaatg atcagcttat
    24121 ccatattttt cctcctttga gcatcagttc tggaattggt ttattgaaac ttcggagaca
    24181 taaaattaag cgatcgatca ccgccagatt cttttaggca aacgcatagc tttgcggggg
    24241 gccagtggaa tagagaaaac gaacatgggc cataccgcct aggataactt taattttctt
    24301 gcggcatctt ctcggaatat tttccctgga agaaatcgca ccggtagtgt aggttgaatt
    24361 ttgaccctaa gtcaagcaaa agttcccgaa attgagaatg atccatgttt ttgtcagggt
    24421 ttatggttta gagaagaggt cggtatggaa tagagaccga ctttgcggcg gtatcgctgc
    24481 cggggtggac ggaaccgggc attcgaaaaa attgataaat tttgttggaa ttcttttcct
    24541 tttcgtgata atgatatatc ctaggctaag gagggtacat tgccaaaaca ccggggagct
    24601 tataaaagcg aaaagcggcg aaaagaactg acccgccaga aaaagcagga agagagaagg
    24661 cagcgcaagt ttagcaaagg agatcacccc ccgtcggacg ctgaaaaggt tgagcaggaa
    24721 gagaaccagc cggccaatcc ttgacttgac acgcctttcc ggggaaaaag acttcctaat
    24781 ccatatttac cacgaacacg acagagccaa cccctcctgt cagggcgggt ttgcttttat
    24841 tataaatggg taaggctgac gaaatcggtc gaaagggggc cgcaggtaag ggcttcccat
    24901 catggccggg gtttcttgcg gagtggaatg agggatccta ctagcccatt ctccccaacg
    24961 actcggacat tcgacaaaga acaggaaacg gagcgaacgc tgcaaagcgg tcgctgccca
    25021 tcaaagtacg tcgcccgaag cgacgcagaa aaggagcggt agagagtatg gctaagaagt
    25081 tattcgtagg ggggttgagc tgggacacga cggacgacgg tctgaaaaac gccttcgcgg
    25141 cccatgggga aattaccgaa gccaaggtca tcaccgaccg ggatacgggg cgctcccggg
    25201 gctttggatt cgtaaccttc acccaggatg aggatgccaa aacggctata gccaagatgg
    25261 atggaacgag cttggacggc aagaccatca aggtcaacga ggcccaggaa aagagccccc
    25321 gcgggggcgg ccggcccggc ggcgggcgcg gcggcggttt tggtggcggc gggcgcggcg
    25381 gcgggcgtga ccgctggtag cctctcaatc aacggggaat atttttttcc tcaagctttt
    25441 taacggtttg acaaaaattc cggggatcca tcgctggttt taggaaaaga agcggtgtgt
    25501 ctccggaatg cccttcaaga ataaaatctg ggctgtaaga agcctttatt ccctttcctt
    25561 gcccacccgg agaacgccat aacgatccaa cctgcccccc cctgctccag ggtgagcggg
    25621 gttttctact taaaccttag gcatttagct tctccgcccg cagtcggtgc cgggtttcgg
    25681 cggagaggaa ggccttgcgc aggcggatgc tgcggggggt gacctccacc atctcgtcgt
    25741 ccttgatgaa ctcgatggct ttctccagcg tcagggggat caccggggtc agctggatgc
    25801 tgtcgtcttt gttggaggcg cgcatgttgg tcaatttctt catcttacag ggattgacat
    25861 tgatgtcgct ctcccggtta tgttccccga tgatcattcc ctcataaacc ggctctccag
    25921 gggttaaaaa gagggaaccc cggggctcca ggttgaagag ggcgtaggac acggcctctc
    25981 cctgccggtc ggagaccagc gatccggtca gccgcagcgg aaagctgccg cggtattcct
    26041 cgtagccctt caggtaggag ttcatgatcc cggttccctt ggtgtcggtc aggaattcgt
    26101 tgcggtagcc gatgagggac cgcgagggga tggtgaattc catgcggacc cggccggtcc
    26161 cgtggttcac catattcacc atgcgcccct ttcgccggga gagtttttcc gagaccgccc
    26221 cgatgaagtt ctcgtcgcag tcgatgaaga ggtgctcgat gggttccagg gtcttcccgg
    26281 acttctgttt atagatgacc tgggggcggc ccaccatgag ctcaaacccc tcccgccgca
    26341 tggtctcgat caggatggcc atctgaaact ccccgcggcc tttgacgatg aagttgtccg
    26401 tctcctcggc gtcttccacg cggatggcca cgttgcggag ggactcctta aagaggcgct
    26461 cgcggatctt gcccgactgc acgtatttcc cctcttttcc ggaaaggggg gaggtgttgc
    26521 tggtgaagag catggaaatg gtcggctcgt cgacggagac ccgcggcagg gccttgggcg
    26581 cttcgcgcgt gcagatggta tcgccgatcg ccacgtcctc gatccccgcc aggatgataa
    26641 tgtcccccgg ctgggccgat tccacttctt tcaggttgat cccgtcgtac acctggagtt
    26701 tggagacccg gagggggatg gccttttcct ccctgtccag gcataccatc tcctcgttgc
    26761 atcccactgt gccgtgaaag accttgccca cggccagccg tccgaggtag tcggagtagt
    26821 ccaggtcggc caccagcatc tggaaaggtt catcggggtc atacaccggc ggcgggatct
    26881 tgtcgaggat ggcctggaac aggggagtga gatcccgtcc ttgctcctcc gcggacttct
    26941 gcgcaatgcc ctcccgcccg acagcgtaga ggacgggaaa atcgatctgc tcttccgtcg
    27001 cgtccaggtc gaagaagagg ctgtagattt cgtccagcac ttccttcggc cgggcatcct
    27061 tgcggtcaat cttgttgacc accacgatga tcttcaaccc ggcttccagg gccttcttga
    27121 ggacgaaccg cgtctggggc agggggcctt ccgaggcatc caccaggagg agggcgccgt
    27181 ccaccatcat gagggcccgc tccacctccc cgccgaagtc ggcgtgcccc ggggtgtcca
    27241 ggatgttgat cttgacatcc tgccaggaaa ccgaacagtt cttggcggcg atggtgatgc
    27301 cccgttcccg ctccaggtcc agagaatcca tgactctttc cgccacctcc tggccggggc
    27361 ggaatacgcc gctttgtttg aacatctggt ccaccaaggt ggtcttgccg tgatcgacgt
    27421 gggcgataat ggcgatgttc ctcaatgttg cattttgcgc gcttcttttc attgttttcc
    27481 caatcatggc ttttatcgga taccgaaatc aggatttgag aaatctatgc gggactgcaa
    27541 gggagggaag gcagcaggaa aaactcttat tgccgaggag gtaaaaccgc ccctttgatc
    27601 tttttgattt ccttttttta tcctctactt ctggtcccag tcgttccttt ccggtttcaa
    27661 ctaccctttt caatatatca taccctgtcc aaatgtacag gctccccgga aataaaacgc
    27721 cccttaaata gcccatgcgg gcgcacggca gcgcgttcct accactctta attctattac
    27781 agatccattc catccatctc catgccgcac ttgaagcact tcttctcttg aatctggtat
    27841 ttgagaatct ggaagcccag acgatccacc aggagctggc cgcaacgaaa acaaaaagta
    27901 tcttctcccg ggtcccctgg aacgtttccc gtgtagacat agcgcagccc ggcgtccagt
    27961 ccgatctttc gggcccggtc caggaccttc accgaagtgg gcgagcggtc gagcatcttg
    28021 tacatgggat ggaaacggct gatgtgccag gggacttctt tccccaggga aacgatgaac
    28081 tgcgccaggt cccgaagttc ttcttccgaa tcgttcagcc cggggatgat cagggtggtg
    28141 atctccaccc agacccccaa ttctctcata accttcaggg actttagcac tccttccagt
    28201 ttggccccgc atcgtttctg gtagaactct tcccggaagg cttttaagtc cacattggcc
    28261 ccgtgaagat gggggtggag ggtcctcaag gcctcctccg tcatatagcc gttggttacg
    28321 aaaatattct tgagtccctc tttgctggct ctctgggcga tttcgaaggc atactcgaaa
    28381 tagatggtcg gctccgtgta ggtgtaggca atggttttac accctgtttt cttcgccatt
    28441 gccacgatct cctccgggct cgcatcttgg ccagtgattt ggttctcgtc cctcggcatc
    28501 tgggagatct cataattctg gcagaagtca caccggaaat tacagcccac cgtggcaatg
    28561 gagaaagacc ggctccccgg ggcgaagtgg aacatgggtt tcttttcaat ggggtccacg
    28621 tttcgggaga tgagccggcg atacaccagg gtgtaaaggg ttccttcccg gttctcccgc
    28681 accgcgcaga ttcccttttt cccctccccg atcaggcacc ggtgattgca gagattacac
    28741 ctaaccctct gctcggacag cttctcataa agcatggctt ctttcatggg gttaccctct
    28801 caggtgggtt cgggttccgg agccttcttt ttgccgaaga tttttaccag ccagatcgaa
    28861 agctcgtaga ggatttgcat gggaatcatc atcaggagca tggtgtaagc atccggagtc
    28921 ggagtcaaga cagcggagag aatggagttg atcaaaaggg catattttcg attcttggcc
    28981 agtacctggt acccgatcag tcccgctctc cccaagatca gcatgatcag aggaagttcg
    29041 aaaatcagac cgaaggcaaa gatcaggccg atggaaaaag tgagatactt ggccagtccg
    29101 atcatgggct caagattctc gctgccgtag gtgaccagga agttgattcc taccggcaaa
    29161 accaaaaagt aacaaaaaaa acagccaccg taaaaaagag ctgtcgccga aagaaccacc
    29221 accagggaat aacggttcgg aaaataggaa gccaaaaggg gatataaagc tctccagaag
    29281 ccataaaaga taaaaggcgc cgccaggaat attcccatga agagggccag gcgcagggag
    29341 gtgatgaacg cctccgggag gctgtacatg atgagcggaa gccccagggg cttctggagg
    29401 aggtgaaggg catccttcca gaaaaaatag gagaccgcca tgagggccat ggtgatgaga
    29461 aacatacgga tgagggcccc gcgcaggcct tccagtttgt cgaagacctc gtttcgatcc
    29521 aggtctttta ccttgtcacg ccagcccata ggataaaatt atacgccgca ggggacccaa
    29581 aattcaaatc ccaaactcag aagaaggagg gataaaatcg ttttggtatt tggtgattga
    29641 aatttaggat ttgtttggtg cctggggttt ggcagttgga gtttgattct tcttcagaat
    29701 tcaggccaga atggttcctt tggaaatgac tacaaccccc gactgggtga cggtaaattt
    29761 tcgggcgtct tcttcctggt tgaacccgat ctggtatccc gccgggatcc gaattcgttt
    29821 gtcgatgatc gcccgcctca cccgggcgcc tcttcccact tccactccgt ccatcaggat
    29881 cgaatcttgg acttccgagt aactatgaat tcttacgttg ggagatagga tagagcgata
    29941 taccctcccc ccgctgataa tgcacccact ggagacgatg gagctgaagg atacccctac
    30001 ccggttcgaa ccgtgttctt cgaagaccgt cttggcgggg gggagggggg gataataggt
    30061 acggatgggc cagttggaat cataaaggtt gaactggggg tccacggcga cgaggtccat
    30121 gctggcctca taataggcat ccagagtccc gatgtccctc cagtacttgg cctgcttctt
    30181 attctcatcg cgaaagctgt aggcatagac ccgatcctga ccgaccatgg cggggatcac
    30241 attctttccg aaatcgtgct gggaatccgg gtccttggcg tcctcgatga cccggcgggc
    30301 caggatttcg gtgttgaaga cgtaaacccc catattggcc aggatgagcg cgggatagcc
    30361 ggggatcgtt ttgggcactc caggcttttc ttcgaagccg atgatccgga attcttcgtc
    30421 gacctgcagg acgccaaact gccgagagag gcttttgtcc atctcgatga ccgccgcggt
    30481 gagatcggct tttttctcca ggtgatgggc gatcatctcc cggtagttca tcttgtagat
    30541 gtggtctccc gagaggatga gaatcaggtc ggggcggtcc tgctgcagga cgtaaatatt
    30601 ctggtagatg gcgtcggcgg tccccaggta ccagtcctcc cccacccgct gctgagccgg
    30661 gatcaggttg atgaactcat tcagctgcgg gttgaagatg ttccatccct ggaagatatg
    30721 gcggttaagg gaaagagatt tgtattgggt gagcaggttg atccgccgga cccgggaatt
    30781 caggcagttg ctcagggtga agtcgatgat gcggtaaatc ccgccgaagg ggacggccgg
    30841 cttggcccga tcctgggtga gaggatagag ccgttcccct ttgcctcccg ccaggaccat
    30901 ggccaggatt cgttcatcca acgggagacc tcctcattcc ttctgacttc ccatcttctt
    30961 tcgtctatcc aggatgtctc ggatcttttg cttgagctcg gtgaggtccg aggatttgac
    31021 cacgtaagcc tcggctcccc aggtcatgaa attttccttg tactgagggt aggccgtgtt
    31081 gagaatgacc ggaacctgac ggtccttccc cagcattgca gtcagggttt caattccgtc
    31141 catgaccggc atgcgaatgt ccatcaccac cacttgaggt ttctcttttt cgaacttcgc
    31201 caggccctcc ttaccatccc tggccagaac gactttgtag ccctcctccg caagttcctc
    31261 ctggtacagg cgaagaagac tcaaatcatc gtctatacaa aggacctttt ccacggcgac
    31321 ctcctttcga gaaagaggga aatgtggcgg gacttcgaca cggcctattc tatcacaact
    31381 tcccccgggc ggagggataa aaatcaggaa cttcccctgc gcaaatgaac cgttcgccgg
    31441 ggggaagggg atttcgcttt cctttttgca gaggggctcc agaatccgat ggccaagctc
    31501 gtgctgcacg gaatacgggt ccgggaattc catcacctgg caagtcatag aggaactgga
    31561 agggaatatc cgaagactcg cctcagggtt cggtcttctc tgccgatatt gggggaaggt
    31621 ccttcttctt tccccgccca atccacagga ccattccccc caccaatgcc cctatcagaa
    31681 aactgaccag gaggaccaga acaaggctcg tctcgaggga ccagaagagg aatttaagag
    31741 tcaacggctg gtagttctgg atggccaaaa cgaccagtaa gacaagaaga acgatgatcc
    31801 cgatgagctt ggcattcatc tttgtttcct ttctcggatg gctttattgc tctattataa
    31861 cccatggaag agccttggaa aaaagataat ggaacccaag ggccatagac ccctttggcg
    31921 gccgcccact tcttttcgcc cgtgtacagg ttatggagtc cgcagccgac cccgccccaa
    31981 acgccgccag aatcaatatg attacgatca gaaccacctt gaaacccatc tcccttctcc
    32041 gctccttctc tcaacttccg cactcggccc ccgccagggc gagaaggggc aaaagacctc
    32101 aggaaggctc catggattcc aggagagcaa ccggaaaatt gcgaaatagg tcccccaggg
    32161 aaaatccagc gcctctaccc ccgtctcgga catccaattc ttcccccgta aaaagatttc
    32221 gatacttccg gccagcccat tccccaggaa ctcgtaccca ggtatccttc caagctgctt
    32281 cccccagggg aggctcttcc cttcccccca ggaggtcgac tactaatcgg ggaaccccca
    32341 ccacgatgga atcctctttg aagattcggg cgaaggcaca aaggtgatct tctttttctc
    32401 cctccccctc caaggggaga taggatcctt cggaaaagat tttcggagcc cggcgacggg
    32461 cctgtagggt tcggtagatc aggtagagct tgatccggcc gtcccagctg ttttccaaaa
    32521 gctcccgcgc caagggaacc agattctccc ctgacttttc agtcctttct ttcagcccgg
    32581 acagcaagct ttggcggagc ccaaaatcca cgggccttcg gttgtccggg tccaccaggc
    32641 tgaggtccca gagctcggtc ccttgataaa tgtcggggac gccggggcag gtgagtttca
    32701 ggagaacctg ggacagggag ttccatcggc caaagtaggc cactcgcctc tgaaaggcct
    32761 ggaggtcgct gagaaagcga ttcttccccc ggggctccag caggttcaac acaaaattct
    32821 tgaccgcccc gtcgtattca tcattggggt tgatccagct ggtgtggacc ttggcctcca
    32881 gtgtggcctt gcgcatgtag gccgcgatcc tctcccgaaa ttgggtgaat tcttcggaat
    32941 cgaaggccaa ggggtcttcc ggcgcctcga ccggccaagc ccccaccagg gtctggtaga
    33001 gcagatactc ttcgttgcgg tccggggccg gctgcctgtc cacgggggtc tttttcgagg
    33061 cgttccaccg gccccagcgg ttcacagcag ccctccactc cccgggaatt tctgaaagaa
    33121 catgcatccg ggcgcgcaca tcctcgctcc gtttcgtgtc gtgagtggag gtggcgagca
    33181 gagaatgggg ccatttatcc gaccgctcta tattccggag gtggaattca tccggtgagc
    33241 ccccaaattt ttccgggtgt ccacctacct cattcagaga aaccaggcgg ttgtaaatgt
    33301 agaacgccgt atcctccagc cctttggcca tcaccggtcc ggtgatctgc tggaacttca
    33361 tgacgaattg ggtcaaacct tcccagtcct ccccggaaaa gtgttgcagg tttcgaagca
    33421 gcagagtatc ccggacgaag tcgaagatgg attcggcggt gcgtgggttc cgcctcttgg
    33481 cttcttccac cgccgcttct atgtaggcga cgtcgcgctc cggaacgttt cccgggccgg
    33541 taatgtaagt tcggtaaacg ggcagacagg caatgacttc cctcaggaca aaggtgaggc
    33601 tgttcagggt aaaatcccgg tacccacggt tcttttccga tatccgctcc agttggtggc
    33661 tcaatgccgc tacttcactg gccagagaca ccagcatgat catttttttc catgtattga
    33721 ccaggctgcc aaaggggatt tggacgccca cgaaatggac gtagaccccg tccatcgccc
    33781 cggcgtttcc cccgtccaca aaaaggccgt tcacctggtt gagaaagtca taccccgtcg
    33841 tcccatagac cgcccaatct cctggcaggg gctctccctc tgcgagaatc ttctcggcca
    33901 ccacgtagag gggccagggg ggggctgggg ttctttcccg gattcgcccc agcaaggcct
    33961 ccgcctcctc ttcgaatcga gaggccggtg tctccccctg gggggctaag cgggagcgga
    34021 ggtgatggat caagtagttc ttctgaagct ggagaaagta gtgcctgggg ttccataggc
    34081 cgtcggggtg atcgatccgc aggccggtaa cctttccctc gcccaaaagc cggaaaacca
    34141 ggcgatgggc ctcctcaaag acctgcggga gttccatgcg gatggccgcc agatgattga
    34201 tgtcgaagaa acgccggtag ttgatctcgt cggccgccac ccgccagaaa gctggccggt
    34261 acacctgtgc gctgatcaag gagtccatcc ggtcgaagct gctcgggtcc ccaggctgtc
    34321 cgttaaaagc ctggatctcc gactccaggg cggttcttac ctccggactc ttctgacaaa
    34381 ggctggcaat gcgccgtttg atgacttcct tttccctctt cagctcctcc agtttttccg
    34441 gggccgattc ggtcagggac ggcaggtagc ccagcgcggt cagaatgctc cgaagctcct
    34501 ggaggtcttc attttctttg cccagagttc ggatgagttc ctccagcgga tgctcgagaa
    34561 cctggccgta ggtgcgggga gagaccggga gcttgtgctc ataataagaa atgaagaagg
    34621 cgccttcctc gagcttgagc tggaacttgc cctcctctag gaccttgccg tactgatcct
    34681 cgaggatggg aaggagcacc ttgttctgca tttcgggttt caccggccgg agctcgatgt
    34741 cgaaataaca ggaaaacaca gaactgggcc cgttttccag cacgtccatc caccatgggt
    34801 ttccccggct gatgcccata tggttgggga ccacatccat gaggagtccc atggagcgct
    34861 cgcgcaggga agcggagaga gtctcaaagt cctccggggt tccgacggcc gggttaattt
    34921 ctttatggtc gcacacgtcg tagccagcgc cctccacgtc gcagggcttg aaaaaaggcg
    34981 aagcatagag atcgctgatt ccgagttcat cccaataagg aaccagcgcc agggactggc
    35041 ggaaagtaaa agattttccg agctggactc ggtatgtggc ccgtgggatc ctggcttgga
    35101 gggaggtttt ctcggccaac cggggtaagg tcgaagcagg ttctggcgcc gtctctttct
    35161 tcatttccat ctttttctcg tttctattcc acccggaagt aaagcttgtc gcccagcgaa
    35221 gtgaagtggg ccatccaggt ttaaacctcc tcggtccggg aaaacaggat gaagctccag
    35281 ggatgcaggg ccagggaggc ttgtccttgg gaatgaatca ggctgggatt cagactgccc
    35341 cttcccttcc atttttcatc ctccgagtca atctctttcc gccaactccc ctccccgacg
    35401 ggcaggttca ccaatacccg ggattgtccg aagttgaagg ccatgaaaac tttttcttcc
    35461 cttctccagc gcctgaccca gaggactttt tctttttcca acaccaacgc ttccagatgc
    35521 tccttactta aaaaggccag gggcgaaagg cgcttccgca agtggatcag ctcccggtag
    35581 aattctcgca gaatccggtg gccttcctcc cggcgaaggt cgtggttcaa tttgcagcgc
    35641 cgaaaggtct cttcgctctg gggatcgggc gtttccccct gccactcaaa agcgctgaat
    35701 tcctgccgcc tcccccgccg caccgcttcc accaggtccg ggtcagagtg gctgatgaaa
    35761 tactgaaagg gggcggtctc cccgtattct tctcccatga agaggagggg gatgaagggc
    35821 gagaggagga cggcgccggc agcgagcttg agcccttccc ggggaacgag gaaactcagc
    35881 cgctcgcctc gcatgcggtt tcccacctgg tcgtggtttt gggcgaacac cacgaaacga
    35941 cgggccggga tgggccggga ggagttcccg tggcgccgcc ggcggtggac ggaatattct
    36001 ccggagtaga tgaaaccctc acgccaggcc ttggccaggc cctcgaccct gccgaagtcg
    36061 cggtaatacc cggcccgttc tccggtgaga agcgtatgaa ccgcatggtg gaagtcgtcg
    36121 ttccactggg cgtccagccc gaatcctccc cgttcccgcg accggatggg gcgcgtatcg
    36181 ttgaggtcgc tctcggcgat gagatgaacc cgccggttca gatgatccgc ctgctccttc
    36241 actacaaggg cgaggtcttc caggaacggt tgggcggaga aatccaggat ggcgtggatg
    36301 gcgtcgaggc gcagggcatc caggtgatag tcggtgaccc aggaaagggc gttctcgatg
    36361 aaaaagcgcc ggacttcatc gctctgggga ccgtcgaagt tgaccgcctc cccccacgga
    36421 gtcctgtagc gatccgtgaa atacggtccg aaatcccgca ggtaattgcc ctccggcccg
    36481 aggtggttat agaccacgtg gagagtggca gccaggccct tctggtggca ggcgttgatc
    36541 agttttttca atccagccgg ccccccataa gaatcttggg gggcgaaagg atagacccca
    36601 tcataccccc aattccggtc cccgggaaac tgggccaccg gcatgagctc cacggcggtg
    36661 atgcccaagt ccttcagctc atccagatga gcgatgacgg cttcgaaggt cccctgggcg
    36721 gtgaaggtgc ccacgtggat ttcgtagatg acataatctt tgagaggaat tccggaccag
    36781 caagaatcag cccacgggaa ggccgggtcg actacctggg aagggccgtg aacgcccttg
    36841 ggctggaaac gggaagccgg gtcgggacgt tcgatccggc cgtccaggct gtatagataa
    36901 aggcttcccg gctccacccc ttccagggtc gcctggtggt agccgtcctt ttcttccaaa
    36961 tccgccaccc gctcctcggg ggaaaggagg agaacctcca ccctgcgggc gcggggtgcc
    37021 cagactgtga accgagaccg gcctccgccg agataaacgg cgcccagggt gatctccggc
    37081 ttcaccttcg ccccccttcg ctcttcaaaa agacgggtga cagattttca tacaggcgaa
    37141 aataagttcc ctggttgaac aagtagagtt catcctccgt aagcagggtg actttatacc
    37201 ttaccgattc ggccatttca gacccttcga gtcataagag ctttacgatt ggaaagcgaa
    37261 catcgattcc tctaggtcga aagagtgcgt acaattgcat tttattcatt tttcaaaaag
    37321 tttgcaaaac cccatatctt gcccttgacc tctaatcaaa tgtattcaca aaaaaagaaa
    37381 cccctaccag gatagcatgc acacgaggtg aaaagagggt caagccaacg cctttgcttt
    37441 acccaacaga tgcagagaga atatggcaca aggggccttg ccctccagct gattgccatt
    37501 cgcaaaaagg ttgactctct ttttcggttc cgtatattct tatacagtgg caaccttttc
    37561 aaaatcataa cccattaact ctcgcattcg atccaacggt aagagcggaa agatattcct
    37621 ttgtttcccg tcccaggcga acatcgatgg gaggatcttt taggatgaac ccaatgggtt
    37681 taaagatcat tcccgccctc caaatctggg gggccctctt tctgggcatg gtggcttttg
    37741 cggctgagcc ctccgagcct gccacagtag gagtcgggat tctgccggtg tacgccggac
    37801 cttcggtgac cagcggcatc gtaaagttct tgaaaaagga ggaagggatc gaggttcaat
    37861 cggagctttt caatccggag ggaaaatggt gcgccatcgt ggagccgtcc caaaaaaccc
    37921 atttgggcta cgtgaactgc gaaggcttga tttttcccgg gccacaaaag cagacgtcgg
    37981 ctcggggtga agaaagccgc cctctggcct ctctcaagcc cgaacccatc cggcctttgg
    38041 cccgttctac atccactgaa tcccgggaag aaaagcaacc cgctcccgcc tttggggaat
    38101 tcctgcaggc cctttggcag gaagacctgg ccagggtcga aaatatgctg aaggcgggga
    38161 tcgatccaaa cacccaaacc accatgggaa accggccttt gctgatcgcc gccaagaaga
    38221 ataacccgag attgctggaa atattgattg ccaaaggcgc caatgtggac gcagcggacc
    38281 taaatggatt gaccccttta atggccgcgg cttccatggg gttggatcaa aacgtgcgag
    38341 tcttgatttc ggcgggtgcc aaggtgaatg cccgggatga aaaggggtca acccctctca
    38401 tctgggcggc catctcgggg cacccccagg tggtggaaat ccttctggcc catggcgcgg
    38461 atgggaaggt aaaaaacaaa gacggccata ccgccctgag tttgtccaag agaatcaacg
    38521 ccagccgcaa gaggtccctc gccgaggcgg aggaggcaaa ccggaaagag gagttggccg
    38581 agcttcggaa aggcctggca agacacgaag ccgtgcttct tctgctggaa aaacctgagg
    38641 aaagatgacc ttatctggaa tccaggaccg ccatccaaat tttccttccg gcatccttct
    38701 ccattttcca aagcgcccca aaaagtcttt ccccgaactt gccgggaagg cattcaccgc
    38761 gctttgcagc cgggatgctg atatcctcca ggaaacggga acggacctga tcctcgcttc
    38821 cttcgcccat gccgcccggg aagcggcgga gaccctcatg gcagaaacct gcatcgaagc
    38881 aacctacaac caagcgtcgg aatcacttgt agagaaaaac ggctggataa ccagcccaaa
    38941 aaagccgggg gagaacccct agactcacca tccaggtgcc tttcccttcg cctcttcccg
    39001 ccttcaagct ttggaagggc ctgcgatcgg tctggttgcc tgttttcctg gaagagccag
    39061 gtctggaaat gcgcccaccc ttttttccca tgctttggtg gaagacggaa aggttcataa
    39121 ttgggtttca gaggggactc gcttttgtta taatctatgg ggggacagga tctcaggctc
    39181 atcgctcaat cacaccaatt cgttcaggag gatttgctat ggaagaggat gtgatcatga
    39241 ctcccgtcgg agttctcatg gtggagcatc tgctcatcgc ccgaatgatg aacttgatgg
    39301 agaggaagct ggcgggaatc aaaccggaga ccaagccgga catggtcttc atcgacgggg
    39361 ccatcggttt tgccaaaacc tatgccgatg cctgtcatca cgggaaagag gaaagcatct
    39421 tttttgataa gctggccatg aagaaccttc ttcctgagca taaaaagctc atggacgaac
    39481 tggtcctgga gcatattcag acccgaaaga tcgtcgccaa cttggagatg gccagagaag
    39541 atttcatgaa gggggactcc ggcgccgtgg gaaggattct gatcatatgt aaaagcctct
    39601 ggcaaatcta tccggcgcac ctcgagaagg aggagaaagc ctttttccct ccctccctgg
    39661 aatactttag caagcgggag caggaggaga tggtgaagca gttctgggaa ttcgacagga
    39721 accttctcct ggaaaggtac attaaatttt tcgacctgta cgacaggtag gagcaaaaag
    39781 gacccaccgg ggtctcctcc cttccatatc cctaccctcc ttgttgaaac ttaaggctcc
    39841 ttggggaaaa tatcaatccg gcacgttgcg gataaaagcc gcggtctcct cggggagagc
    39901 gacgttgat
//
LOCUS       DASPRR010000498         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1087504, whole genome shotgun sequence.
ACCESSION   DASPRR010000498 DASPRR010000000
VERSION     DASPRR010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087504"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VEL68_18380"
     CDS             complement(<1..268)
                     /locus_tag="VEL68_18380"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HXZ37998.1"
                     /translation="MSPNEKSNRDDRVVTSTCSFDCGSRCLLKVHVSEGRAQKITTDD
                     QPGPGLKACARGLAQRDVLYAGDRLKTPLRRVGERGSGEFEPISW"
     gene            complement(265..840)
                     /locus_tag="VEL68_18385"
     CDS             complement(265..840)
                     /locus_tag="VEL68_18385"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXZ37999.1"
                     /translation="MPSAFTHAFIALPLGKTFFEREMPLRFWLLAIFCSILPDMDVYS
                     FYFRTGYGGLFWHRGLTHSLPFAFLLSLAAVWLAFREGNALYLPRWKLWLFFFLLACS
                     HGALDALTGRGYGVAFFSPFDTNRYSFPWAPIRISPIGLKSFFSPWGREVILSEVRWV
                     WVPAVLLLLVVRAFRLMRSRGLLRMSRGFEE"
     gene            complement(1005..1526)
                     /locus_tag="VEL68_18390"
     CDS             complement(1005..1526)
                     /locus_tag="VEL68_18390"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXZ38000.1"
                     /translation="MGALEFFRPSLKTLHGSLMDAVKDLTDEQLNFRPLGKGNHIAFI
                     FWHSVRTEDSVMNFLLQKKPPVWNAEAWDKKFGMDSRAQGTGMSSEQAAALRLRNFAD
                     FLKYAEKVFKASEAYLENLKEEELDRVEEFQFLGKRNFGSVIGGVVLNHGANHLGEIW
                     YVKGLQGLKGSPI"
     gene            1802..2923
                     /locus_tag="VEL68_18395"
     CDS             1802..2923
                     /locus_tag="VEL68_18395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXZ38001.1"
                     /translation="MISPNQDRLIKQFVDFVQIDSPSFEEVRFVSVLAEKLMNLGLAV
                     ENDRTGQNGAGNLLAVFPGTNRNLRPILLSMHTDTVEPGRGIQPRLENGQLRSGGPTI
                     LGADNKSAIAATLEVIRWLQSARPQHGDIELLFTWGEERGHRGAKAFNTSRLRSRIGF
                     VPDGGGPLGTIITAAPYYNSIRAAFIGRAAHAGISPEKGISAIVMASKAILRMKLGRI
                     DEETTANLGMISGGSGRNTVPELVEVEGEARSLILEKLEEQVTNMRSAMEDAAREAGG
                     KVELVNRREYDGFSIDREAAPIPIALAASKDVGLTPSITSTNGGSDANEFNAKGIQTI
                     VLGMGGQGYHSTQESITVEELVKLAEWIGALVIQAGTTR"
     gene            complement(3060..4046)
                     /locus_tag="VEL68_18400"
     CDS             complement(3060..4046)
                     /locus_tag="VEL68_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ38002.1"
                     /translation="MNNGPLLSVQGLKKHFLLKRTRLLGPRERVYAVDGVSFDIQRGQ
                     TLGLVGESGCGKSTLGRAILHLIEPTAGKIFFDGRDITDLTSSELKTLRSQMQIIFQD
                     PYSSLNPRLNVEHIVGDALRTHGLASGREVPDRVAGMLDKVGIHREKMRCFPHEFSGG
                     QRQRIGIARALILQPKLIVCDEPVSALDVSIQAQVINLLGDLKKEFQLSYILIAHDVS
                     VVNHVCNRIAVMYLGKIIELASNKKLLRRPLHPYTQALMSAVPVLDPDQKKRRIILKG
                     DVPSPVNPPSGCRFHPRCFKAMEVCSRVEPLWAEVEPEHFVMCHLYGACHPQ"
     gene            complement(4043..>4430)
                     /locus_tag="VEL68_18405"
     CDS             complement(4043..>4430)
                     /locus_tag="VEL68_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ38003.1"
                     /translation="MLNLQKKINMSILMITHNLGVIAEAANRVIVMYCGKIVESAHVR
                     SIFHNPMHPYTRQLLASVPHLGVDQTRLQEIPGVVPSLYNLPPGCEFHPRCAEATEGC
                     KFRRPEIKEMEPGHWVACDRAVGRGA"
BASE COUNT         1032 a         1264 c         1203 g          931 t
ORIGIN      
        1 cccaggagat gggttcgaat tccccgcttc ccctctcccc caccctcctc aggggcgttt
       61 tcagccggtc cccggcatat aagacatcgc gctgggcaag gcctctcgcg caggccttga
      121 gcccgggacc cggttgatca tcggtggtga ttttttgggc ccgcccctcg gaaacatgaa
      181 ccttgagaag acagcgcgac ccacaatcaa aggaacacgt ggaggtgaca acccgatcat
      241 cccggtttga cttttcgttg gggctcattc ttcaaatcct ctggacatcc ggagaaggcc
      301 tcgggacctc atcaacctga acgcccggac cacaagcaac agcagcacag cgggaaccca
      361 aacccatcgg acctcgctca ggatcacttc tcttccccag ggactgaaaa aggatttcaa
      421 gccgatgggt gagattctta tgggggccca gggaaaggaa tagcggttcg tatcgaaggg
      481 cgagaaaaag gcgaccccat agccccttcc ggttaaggca tccaacgcgc cgtgggaaca
      541 ggccagcagg aagaaaaaga gccacaactt ccaacgggga agatagaggg cgttgccttc
      601 tctaaaagcc aaccaaactg cagccaaact caagagaaag gcaaatggca aggaatgagt
      661 gagcccccgg tgccagaaaa gccccccgta cccggtccta aaataaaagc tgtacacatc
      721 catatccggc aggatggaac agaagatcgc cagcagccaa aagcgcaggg gcatctccct
      781 ttcgaaaaaa gtcttcccca aaggcagcgc gataaaagca tgggtgaatg cggaaggcat
      841 aaaaacaggt tagaggcaca aggattgagg ctcgagaaag aatgaattcc tttctacctc
      901 gagtctcgtt cctcgatcat ttttgttagg tttcttgatg cttcaagcga agcggtcctc
      961 gagtcacgct tcagtccaga cgatcctcaa gcgatgcgat cgttttagat tgggctgccc
     1021 tttaatccct gcaacccctt cacgtaccaa atctctccca ggtggttggc cccgtggttc
     1081 aaaacgaccc ctccgataac cgatccaaag ttccgcttgc ccaggaattg aaactcctcc
     1141 acccggtcca gttcctcttc cttcaggttt tccagatagg cttcgctggc cttaaatacc
     1201 ttctccgcat atttcagaaa atcagcaaag ttgcggaggc ggagggcggc cgcctgctcg
     1261 gaactcatcc ccgttccttg ggcacgggag tccatgccga acttcttatc ccatgcctcc
     1321 gcgttccaca cgggaggctt tttctggagc aaaaagttca taaccgaatc ctcggtgcgg
     1381 acggaatgcc agaagataaa ggcgatatga ttgccttttc ctaggggccg aaaattaagc
     1441 tgttcatcgg tcaggtcctt caccgcatcc atcaggcttc cgtgcagagt cttcagggac
     1501 ggacgaaaaa attccaacgc tcccatggct tttctccttt catggattca agagagactt
     1561 tttaatccta ttcagaattt gaagaaaaaa caactctttt aagggctatg aagacttgag
     1621 gaattcgcaa aacgacgaaa ttaaatagaa ttcgtcactc cggcgaaaac cggaatccag
     1681 ctatttaaag atattcaggc cccaggcact ttttacgaga tcatcgatat tgacctattc
     1741 ccctttttct tttacgattt cctcatgaaa tacctgcctt ttcccggaga aaggattttt
     1801 tatgatcagc ccaaatcagg accgtctgat aaaacaattc gtggatttcg ttcagattga
     1861 ctcaccttcc ttcgaggagg tgcgtttcgt atcggttctg gccgaaaaat taatgaactt
     1921 ggggctggct gtcgagaacg atcgaaccgg gcagaacggc gcgggaaacc tccttgccgt
     1981 ctttccggga acgaaccgaa acctccgccc gattcttctc tccatgcaca cggacacggt
     2041 ggagcccggg cgggggatcc agcctcgtct ggaaaatggt cagctgcgaa gcggcggacc
     2101 caccatcttg ggagcagata ataaatcagc cattgcggcg actctggagg ttatccggtg
     2161 gttgcagtct gcccgccccc agcatggcga tatagagctg ctcttcacct ggggagagga
     2221 acgagggcac cggggagcca aagccttcaa cacctcgcgc ctccggtccc gcataggctt
     2281 tgtcccggat ggcggaggcc cgctgggcac gatcatcacc gccgccccct actacaactc
     2341 catccgcgct gcctttatcg gcagggcggc ccatgccggg atttccccgg agaagggaat
     2401 cagcgccatt gtcatggctt cgaaggcgat cctccggatg aaactagggc ggatcgatga
     2461 agaaaccacg gccaacctgg ggatgatctc cggaggttcg ggaaggaata ctgtgccgga
     2521 actggtggaa gtcgaagggg aggcaagaag cctgattctc gaaaaactgg aagagcaggt
     2581 aacaaacatg cgctcggcca tggaagacgc agcccgcgag gcgggaggca aggtcgagct
     2641 ggtaaatagg cgggagtacg atggtttttc catcgatcgg gaagcggcac ccatcccgat
     2701 cgccctggct gcctccaaag atgtgggatt aaccccttcg atcaccagca cgaacggggg
     2761 cagcgatgcc aacgagttca acgccaaagg aattcaaacc atcgtcctgg gcatgggcgg
     2821 ccagggttac cacagcaccc aggagtccat caccgtggag gagctggtta agctggcgga
     2881 gtggatcggg gctttggtga ttcaagccgg aacgactcgg taggaaagtt ttgagtttta
     2941 ggtttcgagt ctcgagttga aagctaaccc ccgagctcag cgccccccta ctctccacac
     3001 cctacacctt atccctgtat ttttaactcg aaacccgaaa ctcgcaacta gaaactgttt
     3061 tattgggggt ggcacgcccc gtacaggtgg cacatgacga agtgttccgg ttccacctcg
     3121 gcccagaggg gttctacccg agagcagacc tccatggcct taaagcagcg gggatggaaa
     3181 cggcagccgg agggtggatt gaccgggctg ggaacatctc ccttcaggat gatccgcctc
     3241 ttcttttgat ccgggtccag gaccggcacg gcggacatga gcgcctgggt ataagggtgg
     3301 agcggtcttc tgagaagctt tttgttgctt gccagctcga tgatcttccc taaatacatc
     3361 accgcgatcc ggttgcacac gtggttcacc accgagacgt catgagcgat caggatgtaa
     3421 gacagctgga actccttctt caggtccccc agcaggttga tcacctgggc ctggatggaa
     3481 acatccaggg cgcttaccgg ctcgtcgcag acgatcagct tgggttgcag aattaaagcc
     3541 cgggcaattc cgatgcgctg gcgctggccc ccggaaaact catgggggaa gcacctcatc
     3601 ttttcccgat gaatgcccac cttatccagc attcccgcaa cccggtccgg cacctccctc
     3661 ccagacgcca gaccgtgggt tctcaaagcg tcccccacga tatgctccac gttcaggcgg
     3721 ggattcaagg acgagtaggg gtcctggaag atgatctgca tctgggaccg gagggtcttc
     3781 aattcgctcg atgtcaggtc ggtaatgtcc cggccgtcaa agaagatctt tccggccgtg
     3841 ggctcaatta gatggaggat ggccctcccc agggttgact taccgcaacc cgactcgcca
     3901 accagaccga gagtctgccc tctttgaatg tcaaagctaa ccccgtccac cgcgtagacc
     3961 ctttctctcg ggcccaagag cctggtgcgt ttgagcagga aatgtttctt caggccctga
     4021 acgctcagga ggggtccgtt gttcatgctc ccctcccgac ggcccggtcg caggctaccc
     4081 agtggccggg ttccatttcc tttatttccg gtcgccggaa tttgcagccc tctgtggcct
     4141 ccgcacaccg gggatgaaat tcgcaccccg gggggagatt gtagaggctg ggcaccaccc
     4201 cggggatctc ctggagccgg gtctggtcta cccccagatg gggaacggag gccagaagtt
     4261 gccgggtgta agggtgcatg gggttatgaa agatggaacg tacgtgcgcc gactccacga
     4321 tcttcccaca gtacatcacg atcacccggt tggccgcttc ggcgatgacc cccaggttat
     4381 gggtgatcat caggatactc atgttgattt tcttctggag gttgagcatc
//
LOCUS       DASPRR010000499         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_930989, whole genome shotgun sequence.
ACCESSION   DASPRR010000499 DASPRR010000000
VERSION     DASPRR010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930989"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1451)
                     /gene="larC"
                     /locus_tag="VEL68_18410"
     CDS             complement(297..1451)
                     /gene="larC"
                     /locus_tag="VEL68_18410"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HXZ38004.1"
                     /translation="MKILYFDCFSGISGDMTLAALLDLGLPQSKLVEELKKLGLENYS
                     LEIRAGNRSGIGALSLEVRVGPHEEHHRHFSDIQAMIRKSTLDSRVKETSLAIFQRLA
                     EAEARVHKKKVDEVHFHEVGAIDSIVDIVGTAIGLHYFQPDRVYASELPMGRGFVESQ
                     HGRLPLPAPATLEILKGYPVKSVEVEGELVTPTGAAIVAALSSPVTSFPSLAVQKIGY
                     GMGKKEFPDRPNLLRLVLGESSDAYQVDQAVVLESNIDDMNPEFYEHLMDRLFQKGAL
                     DVSLAPLQMKKNRPGTLLRVIAEEKDAEPLAELILQESTTLGVRSYPVKRKKLPREVR
                     EVETRYGKVRVKVSGEVRFQPEYDDCRRIALEKGVPIQEVYWEAMKKRKE"
     gene            complement(1710..2459)
                     /gene="larB"
                     /locus_tag="VEL68_18415"
     CDS             complement(1710..2459)
                     /gene="larB"
                     /locus_tag="VEL68_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HXZ38005.1"
                     /translation="MNVDHLKKLLERVQGGRVPVEKALQELKHLPYEQIDCATIDHHR
                     HLRQGAPETIFGEGKTAAQVLSIMKKMKDKRNNILVTRLDREKVPSVRRVFPKARYYP
                     SPRVLTLIHHPIEIVGKGTILVVCAGTSDIPVAEEAILTARMLGSRVEHLYDVGVAGI
                     HRLMSQRERLMAASVLIVIAGMEGALPSVVGGLVDRPVIAVPTSVGYGSHFGGITALF
                     AMLNSCASGVTVVNIDNGYGAAFAASQINRL"
     gene            complement(2546..2869)
                     /locus_tag="VEL68_18420"
     CDS             complement(2546..2869)
                     /locus_tag="VEL68_18420"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HXZ38006.1"
                     /translation="MGIVTVEDILEEVVGEIEDEYDINQPFYRKMGPRKLWINARMEI
                     DHINETLKLGLPRGDYETLGGFVLERLGRVPLAGEVFRYKKMLFEVLKSNEKSVQEVR
                     VTLKE"
     gene            complement(2857..>3498)
                     /locus_tag="VEL68_18425"
     CDS             complement(2857..>3498)
                     /locus_tag="VEL68_18425"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HXZ38007.1"
                     /translation="ILILGEILPKALFQQKADSWVRRLAPAVWAASILFYPFVWFMSK
                     ATLAVSHFLKQEGEVRPLVTREELQLLLREEGERSDINPLEQAMIRRIFRFPTTKVRE
                     AMIPLVEVVALEETCTVEEAIASVRRENYSRYPVYRNRVDNIVGMWTISWGFCIVLIS
                     LERKGRGKTSGLISAPSPFFRRRNRSMNCWWRCSEARKPWPQWWTNTEGPWAL"
BASE COUNT          748 a         1049 c          883 g          818 t
ORIGIN      
        1 tggcgccggc catttccttc ccgggcggat tcgtcgtatc ggcctgcaag tattggctga
       61 gattctgtac ggcttcttcc ttcacctttt cccagtcaaa attcatcgat tcatccgctc
      121 aaatacattt caccacagag gcatagagaa cacggagaaa cagaattctt ggcaataaac
      181 catgccacga aaataaatca taaccattat tgcttccgga cctaaagcct gcacaggatc
      241 ggagcatttg ttttttgcca taaccctctg tgttctctgt gtctctgtgg tgaatattat
      301 tccttccgtt ttttcatcgc ttcccagtac acttcctgaa tgggaacgcc tttttccaga
      361 gcgatcctcc ggcaatcatc atattccggt tgaaaacgaa cttctccgga cacttttacc
      421 ctcactttcc cgtaacgggt ctccacctcc cgcacctccc ggggcagctt ctttcgcttc
      481 accgggtaac ttcgcacgcc cagcgtagtg gattcctgca aaatcaactc ggccagcggt
      541 tccgcgtcct tttcctcggc aatcacccgc aaaagcgtgc cggggcggtt ctttttcatt
      601 tgcagggggg ccaaggagac atccagcgcc cccttctgga aaagacggtc cattagatgt
      661 tcgtagaact ccgggttcat gtcgtcgata ttcgattcca acacgaccgc ctgatctacc
      721 tggtaggcat cggaagattc gcccaggact aggcgaagaa ggttcgggcg gtccggaaac
      781 tccttcttcc ccatcccgta accgatcttc tggactgcaa gggacgggaa agaggtcacc
      841 ggggagctca gagcggctac gatcgctgct ccggtggggg tcaccagttc tccttccacc
      901 tccacgctct tgaccggata tcccttcaag atttccagcg tagccggagc cggcagggga
      961 agtcggccgt gctgggattc gacgaagccc cttcccatgg gcaattccga agcatagacc
     1021 cggtccggtt gaaaataatg gagcccgatg gctgtgccca cgatgtccac gatggaatca
     1081 atggctccca cttcgtgaaa atgaacctca tccaccttct tcttgtggac cctggcttcc
     1141 gcctctgcca ggcgctggaa gatggcaagg ctggtctctt tcaccctgga gtccagggta
     1201 ctcttgcgga tcatcgcttg aatgtcggag aaatggcggt ggtgctcttc atgcggtccc
     1261 acccggacct ccagggacag cgctccaatt ccgctccggt tcccggcacg aatttccaaa
     1321 gaataatttt caaggcccag ctttttgagc tcttccacga gtttcgattg ggggagcccc
     1381 aaatcgagga gggccgcgag agtcatatcc ccgctgatgc ctgagaaaca gtcaaagtaa
     1441 agaattttca tggaaacccc taaaaatagc tatccgctct ccgtagagct tctagtagat
     1501 gtcactcact ccgtcattcc ggcgaaagcc ggaatccaga atcctttgaa tcccgccttg
     1561 ggcgggactg gattccgggt cgcccccggc tttcgccggg gttgcccgga atgacgactt
     1621 ccgctcgctt tcaagaattc tgcaagaggc tccttattaa gggaaaaaat ctttcgctga
     1681 aagctgaccg ctgaacgctg accgcttctt tatagcctgt taatctggct ggcagcgaag
     1741 gccgcgccgt agccgttgtc gatgtttacc accgtcaccc ccgaagcgca ggaattgagc
     1801 atggcaaaaa gggcggtgat gcccccgaag tgggacccgt accccacgct ggtgggaacg
     1861 gcgatgaccg ggcgatccac caacccgccc accacgcttg gcaaagcacc ttccatcccg
     1921 gcgatgacga tgagaacgct ggcagccatc aaacgttctc tctggctcat gagccggtga
     1981 attcccgcga cgcccacatc gtagagatgc tccacccggc ttcccagcat ccgggcggtc
     2041 agaatggctt cttcagccac gggaatgtcc gaagtcccgg cgcagaccac cagaatcgtt
     2101 ccttttccca cgatctcgat gggatggtgg atgagggtca gaacccgggg tgaaggatag
     2161 tatcgcgctt tggggaaaac ccgcctaacg gaagggactt tctcccggtc caggcgagtg
     2221 accaggatat tgtttctttt atccttcatc ttcttcatga tggagaggac ttgggcggcc
     2281 gtctttccct cgccgaagat ggtctcggga gctccctggc gcagatgacg atgatgatcg
     2341 atggtggcac agtcaatctg ctcgtagggc agatgtttga gctcctggag ggctttttcc
     2401 accgggaccc tcccaccctg aactctttcc aataactttt ttaggtgatc aacgttcatc
     2461 ttaccctcgc cctataatga cttccttctc cctttgaaag aacgggagat gagaaaaatg
     2521 tattgcatga aagagacaga accatctatt ccttcagggt caccctcact tcctggactg
     2581 acttttcatt ggatttcagg acctcaaaga gcatcttttt gtaccggaaa acctcgccgg
     2641 ccaggggaac cctccccagc ctctccagaa cgaatccccc cagggtctcg taatcccccc
     2701 tgggcaggcc aagcttcagg gtttcattga tgtgatcaat ctccatgcgg gcgttgatcc
     2761 atagctttct gggccccatt tttcggtaaa agggctggtt gatgtcatac tcgtcttcaa
     2821 tctccccgac tacctcttcc aggatgtcct ccaccgtcac aatgcccacg gcccctccgt
     2881 attcgtccac cactgcggcc agggtttcct ggcttcgctg catctccacc agcagttcat
     2941 cgaccggttt cgtctccgga aaaaaggaga tggggcggat ataaggccgg atgtttttcc
     3001 cctccccttc cgctccaagg agatcaagac tatacaaaat ccccacgata ttgtccacat
     3061 ccccacgata ttgtccaccc ggttgcgata gaccggatac cgggaatagt tctcccggcg
     3121 gaccgaagca atggcctcct caacggtgca ggtttcctcc agggccacca cctccaccag
     3181 gggaatcatg gcttccctta ccttggtggt ggggaaccga aagatccggc ggatcatggc
     3241 ctgttccagg gggttgatgt cgctccgctc cccctcttcc ctgaggagca gctgaagttc
     3301 ttcccgagtg accaggggcc gcacttcccc ctcctgcttg aggaaatggg agacggccag
     3361 ggtggctttg gacatgaacc atacaaaggg gtagaaaagg atcgaagccg cccagactgc
     3421 gggggccaat cttctcaccc aggaatcggc cttctgctgg aaaagggctt tgggcaaaat
     3481 ctcccccagg atgaggat
//
LOCUS       DASPRR010000500         9999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_123486, whole genome shotgun sequence.
ACCESSION   DASPRR010000500 DASPRR010000000
VERSION     DASPRR010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123486"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VEL68_18430"
     CDS             complement(<1..989)
                     /locus_tag="VEL68_18430"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXZ38008.1"
                     /translation="MKEKVKKVVLAYSGGLDTSVILKWLIETYDCEVIAFAADLGQAE
                     ELTGLKEKGKRTGASKVYVEDLREEFVRDFIFPMVRANAIYEGGYMMGTSIARPLIAK
                     HQMLIARKERADAVSHGATGKGNDQVRFELTYLALNPHIKIIAPWKIWNFKSRTDLIR
                     YAKMHGIPVPVTAAKPYSSDRNLFHISFEGGILEDPWNAPPEDMFLLSVSPEKAPNRP
                     TLVEIEFEKGNPVSLDGKRMSPAKLLAELNRLGGKNGIGRVDIVENRYVGMKSRGVYE
                     TPGGTILHAAHRAVESITMDREVMHIRDSLIPRYSELIYYGYWFSPERELIQK"
     gene            complement(1336..2214)
                     /gene="argB"
                     /locus_tag="VEL68_18435"
     CDS             complement(1336..2214)
                     /gene="argB"
                     /locus_tag="VEL68_18435"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXZ38009.1"
                     /translation="MEKYIEKADILLEALPYIQRFANKTMVIKYGGHAMEDEELKNGF
                     VRDVILLHYIGLNPVIVHGGGPQIDGMLNRIGKKSTFVEGMRVTDEETMDVVEMVLVG
                     QINKEIVSRMNSHGGHAVGLSGADGTLIRARKMWVHKPGKEGQEEERIDLGLVGEVES
                     INPDILRTFIANKWIPVIAPVGVGPGAEAYNINADLVAGKVASALQAEKFILLTDVEG
                     VLNNQKQLISTMDAKMAARFIQEGTISGGMIPKVRCCLDALQEGVKKAHVIDGRVKHA
                     VLLEIFTDEGIGTQIF"
     gene            complement(2306..3730)
                     /gene="hslU"
                     /locus_tag="VEL68_18440"
     CDS             complement(2306..3730)
                     /gene="hslU"
                     /locus_tag="VEL68_18440"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HXZ38010.1"
                     /translation="MSEKGIEREELQPYTPREIVAELDKYVIGQKNAKRSVAIALRNR
                     WRRQRVDKDLRDEIAPKNIIMIGPTGVGKTEIARRLSKLAQSPFLKIEASKFTEVGYV
                     GRDVESMVRDLTELAVNMVRAEEMEKVMVKAREMAEERMLDLLLPTPRRGSPGTDPKE
                     NPSEGAEVGASPELVLANSAKPSQTRERLRKMLREGKLDNRMVELELTQRQIPVVEIF
                     SNAGMEDIDFNLKEMFSSLLPKKTKKRRVKVPEGLELLTEEESQKLVDMDRVSSLAVE
                     KVEQAGIIFLDEIDKIAGRESTHGPDVSREGVQRDLLPIVEGTTVNTRYGMVKTDHIL
                     FIAAGAFHVSKPSDLIPELQGRFPIRVELESLTKADFVRILTEPQNALIKQYKALLET
                     EGIDLSFTEDAVAEIAEIASLVNQRMENIGARRLHTVLERLLDEISFDAPDRSERKIG
                     VDAGYVRKKLEEIIQDEDLSRYIL"
     gene            complement(3988..4554)
                     /gene="hslV"
                     /locus_tag="VEL68_18445"
     CDS             complement(3988..4554)
                     /gene="hslV"
                     /locus_tag="VEL68_18445"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXZ38011.1"
                     /translation="MNERVMGTTIIAVRHKGQIAIAGDGQVTVGNTVLKHGARKVRRL
                     YKDQIVAGFAGSTADAFTLFEKFESKLEQYHGNLSRAAVELAKDWRTDRMLRRLEALL
                     IVADRDHILVLSGTGDVIEPDDGVAAIGSGGPYALAAARALIQYSNLEAKSIVQEAMR
                     IASQICIFTNDQIVMEELGDADPGVQRA"
     gene            complement(4706..5626)
                     /gene="xerC"
                     /locus_tag="VEL68_18450"
     CDS             complement(4706..5626)
                     /gene="xerC"
                     /locus_tag="VEL68_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXZ38012.1"
                     /translation="MKELIEKFLEHLRVERNASAHTIRSYASDLEQFRSFLCSFGVKQ
                     EGEKGDVAVEKIDHLAIRAFLSHLYRGNKKSSLARKLASQRSFFRYLVNEGVLKQSPA
                     EIVATPKLEKSLPSFLPVDEVFALVEAPDPSTLWGARDRAILETLYSCGIRVSELVGL
                     SDGDIDFHLGILRVYGKGGKERVVPVGSKAVEALQAYLPRRDEVLAQRKRTGPGTPLF
                     INPRGTRLTARSVGRILLKHILRGGFLKKVSPHGLRHTFATHLLDAGADLRVIQELLG
                     HVSLSTTQRYTHVSVDKLMEVYDKAHPRAK"
     gene            5813..7903
                     /gene="fusA"
                     /locus_tag="VEL68_18455"
     CDS             5813..7903
                     /gene="fusA"
                     /locus_tag="VEL68_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXZ38013.1"
                     /translation="MKKYEVNRLRNTAIIAHGKAGKTSLSEAMLFDGGSTDRLGRVDD
                     GSSVMDFEPEEIKRKLTISSSFNHLEWDKHKINIIDTPGDANFIIETKNSLQSADAAV
                     VVLDAVSGVEVQTEKVWEYANQFGLPRLIFISKMDRERADFFATLGDIQKVLSPKAIP
                     LNLPIGAEESFSGVVDLLSGKAYQFENNLSGKFTTGDIPANLKDQAKTARDKLIEAIA
                     ESDDALLEKYLEGQELSSEDLANGLRKGVLNKTIFPVLCGSGLKNMGVQPFLDAIVQL
                     LPSPLDRGPAKGTHPSTKAEEARNPKEEEPFSAFIFKTVADPYAGKLSIFRVWSGTLQ
                     ADSTLYNANKGVRERFGQILQMEGKNQKPVESVGPGDIVAVAKLKETTTWETLCDEKK
                     PIVYPTLSLPVPPVSMAVEPKSKGDEEKITTSLFRLCEEDPTVKLQRDEQTKELILFG
                     MGQVHMEVTVEKLKRKFGVDVNLKTPKIPYKETIKSTKSGIIYRHKKQSGGRGQFAEV
                     HFELSPLPRGAGFEFKNALVGMNVPRNFVPAVEKGVNEAMQSGVLAGYPVVDLKVKFY
                     DGKSHEVDSSEIAFKIASSMALRKGVMETNPVLLEPIMNMEVFVPDEYMGDVIGDLNG
                     RRGRVLSVEPRAKGQVIKSQVPLAEVLKYAPDLTSMTSGRGSFSMEFSHYEEVPAHLA
                     EKVIAASKAEQKEA"
     gene            7977..8438
                     /locus_tag="VEL68_18460"
     CDS             7977..8438
                     /locus_tag="VEL68_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXZ38014.1"
                     /translation="MNPRGKVVAVNISARKGTRKKNVGTSCLLPGLGLKEDAHAAPGN
                     RQVSLLAQESIDKMVRLGLKVGPGDFAENITTQGLDLLSLPLGTRFRIGDRTLLEVTQ
                     IGKVCHTRCAIYYQAGDCVMPKEGIFAQVLEGGEIKVGDEVRVLGGNSDHQ"
     gene            8407..8910
                     /gene="mog"
                     /locus_tag="VEL68_18465"
     CDS             8407..8910
                     /gene="mog"
                     /locus_tag="VEL68_18465"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HXZ38015.1"
                     /translation="MFSAGILTISDKGSRGEREDLSGPEIRQVLSSLPARVEAYEVIP
                     DEEEIIARKLIEYADRKGLNLILTTGGTGLSPRDVTPEATRKVLDKEVPGIAEAMRAE
                     GLKVTPTAMLSRAAAGIRGRSLIINLPGSPRAVRENLMVVLPALKHALEKAQGDPADC
                     GNLPRKE"
     gene            complement(8950..9282)
                     /locus_tag="VEL68_18470"
     CDS             complement(8950..9282)
                     /locus_tag="VEL68_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38016.1"
                     /translation="MTLWELIKKGAEDGLEVMKDGLSIFMAEAGKTSRILKKRVELTS
                     VQGNVRKAFIRLGSVAYELQSRGESDIYGQEEVEELIAEIDGYKVRVREIEREIETIH
                     TEEQKKHS"
     gene            complement(9357..9821)
                     /locus_tag="VEL68_18475"
     CDS             complement(9357..9821)
                     /locus_tag="VEL68_18475"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HXZ38017.1"
                     /translation="MKFLADRTTGKLAKKLRALGFDAASWGGGPIQEAARVAVIEGRM
                     FLTRSRRISAAPKDLQVTILEANDPREQVREVLQKLHLKPSDNQYFSRCLMCNEELQP
                     VTKGEVEGRVPDFIYRYYNSFYLCPRCCRVYWPGTHLDHMKEEVDQILSDKD"
BASE COUNT         2076 a         2971 c         2633 g         2319 t
ORIGIN      
        1 aatttttgaa taagctccct ttccggagaa aaccagtatc cataatagat cagttccgaa
       61 taccggggga tcaatgaatc ccggatgtgc atcacttccc ggtccatggt gatggactcc
      121 accgcccggt gggccgcatg aaggatcgtc cctccggggg tctcatacac tccccgggac
      181 ttcatcccca cgtagcggtt ttccacaata tcaacccttc cgatcccgtt cttgccgccc
      241 aggcggttta actcggccag cagtttggcc ggggacatgc gctttccgtc gagcgaaacc
      301 gggttgcctt tttcaaattc gatctccacg agggtgggcc tgttcggggc cttctccggc
      361 gagacgctga ggaggaacat gtcctcgggg ggcgcattcc aggggtcttc caggatgcct
      421 ccctcaaagc tgatatggaa gaggttgcgg tcgctgctgt aaggctttgc cgccgtcact
      481 ggaaccggga tgccatgcat cttggcatag cgaatcaggt ctgtccggga cttgaagttc
      541 cagattttcc agggagcgat gatcttgatg tgcgggttga gagccaggta ggtcagctcg
      601 aagcggacct ggtcgttccc cttccccgta gctccgtggg acacggcgtc ggctctttct
      661 ttccgggcga tcaacatctg gtgcttggca atcagggggc gagcgatgga ggtccccatc
      721 atatacccgc cctcgtaaat cgcgttggcc cggaccatgg ggaagatgaa atcccggacg
      781 aactcctcgc gcaggtcttc cacatagacc ttgctggctc cggtcctctt ccctttttcc
      841 ttcaggccgg tcagctcttc cgcctggccc aggtctgcgg caaaggcgat cacctcgcaa
      901 tcgtaagtct cgatcagcca cttcaggatc accgaagtgt ccaatccgcc ggaataggct
      961 aaaacgactt tctttacttt ttctttcatc tttctcccca tccttctttc ttttttggct
     1021 ttgttcgaaa agtacgtgat tcgttgcgca gcggtcaaaa aaagtgtgaa atttgccaag
     1081 aaatcttgtt tggtcaatcc tctctgaaat cccccccacc cccctttgat aaaggggggc
     1141 aggggggatt tctggcaggg ccatggccct agggaaaatc ctggctgctg agtgcggctc
     1201 tgccaatgtg cgaatatccc caaaagtgat tgattacgca tttttcagac aaaatacttt
     1261 tctcccactg caaagattgc cgagatggca aagtctgggc tacgcttcct aaggctaatg
     1321 gccttttttt gcttttcaaa aaatttgcgt cccaatcccc tcgtcggtga aaatttccag
     1381 cagcacggcg tgtttcaccc gcccgtcgat gacatgggcc tttttcaccc cttcctgaag
     1441 ggcatccagg cagcacctca ctttggggat catccctccg ctgatggttc cttcctgaat
     1501 gaaccgggcg gccatcttgg catccatggt ggagatcaat tgcttctggt tgttcagaac
     1561 cccttccaca tcggtcagga ggataaactt ctccgcctgc agcgcggagg ccactttccc
     1621 ggccaccagg tccgcattga tgttgtaggc ttcggccccc ggcccaaccc cgacaggggc
     1681 tatgaccgga atccatttgt tggcgataaa ggttcggaga atatcgggat tgatggattc
     1741 cacttccccc accaacccca aatcaatccg ttcctcttcc tgcccttcct tgcccggttt
     1801 gtgaacccac attttgcggg cccggatcag ggttccgtcc gcgccggaga gccctaccgc
     1861 atgccctcca tgggaattca tccgggatac gatttcttta ttgatctgtc ccaccaggac
     1921 catctccacc acgtccatgg tttcttcgtc ggtcaccctc attccttcga cgaaggttga
     1981 ctttttgccg atccggttca gcatgccgtc gatctgcggc ccccccccgt gaacgatgac
     2041 cgggttcagc ccgatgtaat ggaggaggat gacgtcgcgg acgaagccat ttttcagctc
     2101 ctcgtcctcc atggcgtggc ctccgtactt gatgaccatg gtcttgttgg caaaccgctg
     2161 gatgtagggc agtgcctcca acaggatgtc cgctttttcg atgtactttt ccatcttaac
     2221 ttctcccaca ttgccgcccc ttcccctcat cccccccgag cgagagaagg aagggaaaag
     2281 gggccctcgt gcatcgagcc tgctcttaca gaatgtaccg gctgaggtcc tcgtcctgga
     2341 tgatctcctc cagttttttc cggacatacc cggcatcgac cccgattttc ctctctgacc
     2401 gatccggagc atcgaaggaa atttcgtcca acaacctctc caagacggta tgaagccttc
     2461 gggcgccgat attttccatc ctttggttga ccagggaggc aatctcggcg atctcggcca
     2521 cggcgtcctc ggtgaaggag agatcgatcc cttcggtctc cagcagggct ttgtactgct
     2581 tgatcagggc gttttggggt tcggtcagga tacggacgaa atccgccttc gtcagggact
     2641 ccagctccac ccggatggga aaacggccct gcaattccgg aatcaggtcc gagggtttgg
     2701 atacatggaa agcccctgca gcgatgaaga ggatatggtc cgtcttcacc attccataac
     2761 gggtgttcac cgtggtcccc tcaacgatgg gaagaaggtc tcgttgcacc ccttcccggg
     2821 aaacatccgg gccatgggtt gattcccggc cggcaatttt gtcgatctca tccaggaaga
     2881 tgatccccgc ctgctccacc ttctcgacgg ccaggctgct cacccggtcc atatccacga
     2941 gcttctgaga ctcttcctca gtgagcaatt ccagcccctc gggaactttc acccttctct
     3001 tcttggtctt cttggggagg aggctgctga acatctcctt caggttgaaa tcgatatctt
     3061 ccattcccgc gttggagaag atctctacga cgggaatctg ccgctgggtc agttccagct
     3121 ctaccatccg gttgtccagt tttccctccc ggagcatctt tctcaatctc tcccgggttt
     3181 gagagggttt ggctgaattg gcgaggacca actccggtga agcgcccacc tccgcccctt
     3241 ctgagggatt ctctttcggg tccgtccctg gggagccccg ccggggggta ggaagcagca
     3301 agtccagcat ccgctcttcg gccatctccc gggctttgac catcaccttt tccatctctt
     3361 ccgcccggac catgttgacc gccagctcgg tgaggtcccg gaccatggat tccacatccc
     3421 ttcctacata tcccacttcc gtaaatttcg aagcttcgat ttttaaaaaa ggggactggg
     3481 ccagtttgga taagcgccgg gcaatctcgg ttttgcccac gcccgtcggg ccgatcatga
     3541 tgatgttctt aggagcaatc tcgtcccgca ggtctttatc cacccgctgc ctccgccagc
     3601 ggttgcgcag ggcaatggcc acggaccgct tggcgttctt ctggccaatc acgtacttgt
     3661 ccagctcggc gacgatttcc cggggggtgt agggctggag ttcttctctc tctatccctt
     3721 tttcactcat atctctctat cgcttttctc ccctaatttg gagactgggt caaaattcgc
     3781 catccgcatt ttgagacgcc cgccctgagc ggactgttca cccttcgaca agcgttcgac
     3841 tgagctcacg ccgaaggctc agggcgaacg gctatgttat tgatttctaa tgtcccccgg
     3901 tccgttcgtg gtgagtctgt cgaaccatgg acggacttac gacacagccc cctaaaaagg
     3961 ggggattctt tatttcttca ctatacccta tgctctttgg acgcctgggt cggcatctcc
     4021 cagctcttcc atcacgatct gatcgttggt aaagatacag atctgggaag caatgcgcat
     4081 ggcttcctgg acgatggatt tggcttccag gttggaatac tggatcaggg cccgggctgc
     4141 agctagggca taagggcccc cactgccgat ggccgccact ccgtcatctg gttcgatcac
     4201 atccccggtc ccagagagaa ccaggatgtg atcccggtcg gcaacgatca ggagcgcttc
     4261 cagccggcga agcatacggt ccgtcctcca atccttggcc agctccaccg ccgcccggga
     4321 gaggttgcca tggtactgtt cgagcttgga ttcgaatttt tcaaaaagag taaaggcatc
     4381 ggccgtcgat cccgcgaatc cggcaacgat ctgatctttg tagagtctac gcaccttacg
     4441 ggcgccgtgc ttcaacaccg tgttccccac cgtcacctgc ccatcccccg ctatggcgat
     4501 ttgtccctta tggcggacag caatgatggt cgttcccatc actctttcgt tcatcgatcg
     4561 atccttattc tctcaccaca gaggcacaga gagcaccgag atatgagatt aatgatgaaa
     4621 ggccaaaaag ccttcactgg gttcgggctt catcttcatg ctaattcttt ctccgtgtcc
     4681 tctgtgcctc tgtagtgaat gttttttact tggccctcgg gtgcgcttta tcgtacacct
     4741 ccatgagctt atccacgctc acgtgggtgt accgctgggt cgtcgatagg ctcacatgac
     4801 ctagcagctc ctggattacc ctcaggtccg ccccggcatc gaggagatgg gtggcaaaag
     4861 tatggcgaag tccgtggggg ctgacttttt ttagaaagcc tccccgcaaa atatgcttaa
     4921 ggagaatgcg cccgacgctc cgggcggtga gccgtgtccc ccgggggttg atgaaaaggg
     4981 gagtgccggg tcccgttcgc tttctttggg ccagcacctc gtctcttcgg ggaagatagg
     5041 cttgcagtgc ctccaccgcc ttgctcccca cgggcacgac cctctctttg cccccttttc
     5101 cataaacccg aaggattccc aggtggaaat ctatgtctcc gtcgctcaaa ccgaccagct
     5161 cgctaacccg gattccgcag gagtaaaggg tctccaggat ggcccggtct ctggctcccc
     5221 agagggttga cgggtccggg gcttccacca gcgcaaagac ctcgtccacg ggcaagaacg
     5281 aggggaggct cttctccagc ttcggagtag ctacgatttc cgccgggctc tgctttaaaa
     5341 ctccctcgtt gaccagatag cgaaaaaagc tccgttgaga ggccagcttt cgggccaaag
     5401 aggacttctt gttcccccga tagaggtggc tcaaaaaggc tcggatggcc agatgatcga
     5461 tcttttctac cgccacatct cccttttccc cttcctgctt gactccaaag gaacataaaa
     5521 agctgcggaa ctgctccagg tcggaggcat agcttcgaat cgtatgcgcg gaagcattcc
     5581 tttccacccg gagatgctca agaaattttt cgatgagctc tttcaccctc gcccctgggg
     5641 actgaaaaga aaagcatctt aaccgaacca agcattcccc ccccatggca aaaaagatac
     5701 cctcgggcct ttccttcagc ccagtcgcac aggttcaact cagggggatt ttagcttgat
     5761 ttttatctta atataaatta ttttaaagat tcaataaaaa taaggagtct ttatgaagaa
     5821 atacgaagta aatcgtttgc ggaatacagc catcatagcc catgggaaag cgggaaaaac
     5881 ctctctttct gaagccatgc tctttgacgg agggtccacc gaccgtctgg gccgggtaga
     5941 cgacggctcc tcggtcatgg acttcgagcc cgaggagatc aagcggaagt tgaccatcag
     6001 ttcctccttc aaccatctgg agtgggacaa acataaaatt aatatcatcg acactccggg
     6061 ggacgccaat tttatcatcg aaaccaaaaa cagcctgcaa tcggccgatg cagccgtggt
     6121 cgttctggat gccgtctccg gagtggaagt tcagaccgag aaggtttggg aatatgccaa
     6181 ccagttcggc ctcccccgct tgatcttcat ttccaagatg gaccgggagc gggccgattt
     6241 cttcgccacc ctgggggaca tccagaaggt cctcagtccc aaggctatcc cgctgaattt
     6301 gcccatcggg gccgaggaat ctttttccgg cgtggtggac ctgctatccg gtaaagccta
     6361 tcagtttgag aacaacctga gcggcaaatt taccacggga gacattccgg ccaacctgaa
     6421 ggatcaggcc aagaccgccc gggacaagct catcgaagcc atcgccgagt cggacgatgc
     6481 ccttctggaa aagtacctcg aggggcagga actttcatcg gaagacctgg ccaacggcct
     6541 gcgcaagggc gtcttgaata aaaccatctt ccccgtcctc tgtggatcgg ggttgaagaa
     6601 catgggggtc cagccctttc tcgatgctat cgttcaatta ttgccttctc ccctggaccg
     6661 tggcccggct aaaggcaccc atccctccac caaggcggag gaggcccgaa atcccaagga
     6721 ggaggaacct ttctcggcct tcatcttcaa aaccgtggcc gatccctatg ccggaaagct
     6781 gagtatcttc cgggtgtggt ccgggaccct tcaggcggat tcgactctct ataacgccaa
     6841 caaaggggtc cgggagagat tcggtcagat cctgcagatg gaagggaaga atcagaagcc
     6901 ggtggaatcg gtaggacccg gcgacattgt cgcggtggcc aaactgaaag agaccaccac
     6961 ctgggagacc ctttgcgacg agaagaagcc catcgtctat cccaccctct ccctccccgt
     7021 tcccccggtg tctatggccg tagagcccaa atccaagggg gacgaggaaa agatcactac
     7081 ctccctcttc cgcctctgtg aagaagaccc gaccgtcaaa ctccagcggg acgagcagac
     7141 caaagagctg attctcttcg gaatggggca ggtccacatg gaagtgaccg tggagaaact
     7201 caagcgcaaa ttcggggtgg atgttaacct gaagaccccc aagatccctt acaaggaaac
     7261 catcaagagc accaagagcg gcattatcta ccgccacaag aaacaaagcg gcggccgcgg
     7321 gcagtttgcc gaggtccact tcgagctctc ccccctgccc cggggcgccg gcttcgaatt
     7381 caagaacgct ctggtgggga tgaacgtgcc ccggaacttc gtccccgcag tggagaaagg
     7441 cgtcaacgaa gccatgcaga gcggggttct ggccggatac ccggtggtcg atctgaaggt
     7501 caagttctac gacgggaagt cccacgaggt ggactcctcg gaaatcgcct tcaaaatcgc
     7561 ctcctccatg gccttgagga agggagttat ggagaccaat cccgttcttc tggaaccgat
     7621 catgaacatg gaggtctttg ttcccgacga atacatggga gatgtgatcg gagacctgaa
     7681 tggccggcgg ggccgggttc tttccgtgga gcccagggcc aaagggcagg tcatcaaatc
     7741 ccaggtccct ctggccgagg ttttgaaata tgccccggac ctcacttcca tgaccagcgg
     7801 ccgagggtct ttctccatgg agttctctca ttacgaagaa gtccccgccc acctggccga
     7861 aaaagtcatt gccgcttcca aggcggagca gaaggaggcc tagctttcgg attccagagg
     7921 gaccgattcg aggtcccagt ctgccgctgc ctcctcttca aacgaaaagg ctgaggatga
     7981 accctcgggg gaaagtcgta gccgtaaaca tcagcgcccg aaagggaacc cggaagaaaa
     8041 atgtgggcac ctcctgcctg cttcccgggt tgggtttgaa ggaggatgcc catgccgctc
     8101 cgggaaaccg gcaggtgagc ctcctcgccc aggagtccat cgacaagatg gtccgcctgg
     8161 ggctgaaggt agggccgggc gatttcgccg aaaacatcac cacccaggga ttggacctac
     8221 tctcccttcc cctgggaacc cggttccgca tcggagaccg gaccctcctg gaagtgaccc
     8281 agatcggaaa agtctgccat acccgctgcg ccatctatta tcaggcaggt gactgcgtca
     8341 tgcccaaaga ggggatcttt gcccaggtcc tggaaggcgg agagatcaaa gtgggggacg
     8401 aggtccgtgt tctcggcggg aattctgacc atcagtgaca agggctcccg cggggaaagg
     8461 gaggacctga gcggacccga aatccgccag gtcctctctt ctcttcccgc ccgggtggag
     8521 gcttacgagg tcatcccgga tgaggaggaa atcatcgccc ggaagcttat tgaatacgct
     8581 gaccggaagg gcctgaattt gatcctcact acagggggaa caggcctgag cccgcgggac
     8641 gtcaccccgg aggccactcg caaggttctg gataaggaag ttcccggaat cgccgaggct
     8701 atgcgcgccg aggggttgaa ggtcaccccc acggccatgc tttcccgggc tgcggccggg
     8761 attcgtggcc ggtccctgat cattaatcta cccggaagtc cgcgagcggt gcgggagaac
     8821 ctaatggtgg tcctgccagc cctaaaacac gccctggaaa aggctcaagg agacccggcc
     8881 gactgtggga atctgccgag aaaggaatga atcggccgcc ttgcatggag ggaaaggcag
     8941 agggcagcct taggaatgtt ttttttgttc ttccgtatgg atggtttcaa tctctctttc
     9001 aatctcgcgt actcttacct tgtagccgtc gatctcagct attaactctt ctacttcctc
     9061 ttgaccgtaa atgtctgatt ccccgcggga ttgaagctcg taggccacac tgcccagacg
     9121 gatgaaggcc ttgcgaacat tcccctgaac cgaggtcagt tccacccttt ttttcaatat
     9181 gcggctggtt ttcccggctt ccgccatgaa gatcgacagc ccgtccttca tgacctccaa
     9241 tccatcctcc gcgcccttct tgatcaattc ccagagggtc attcctaccc tccctccttt
     9301 ctgctctaag gtccctcttg ccgggttttc ctcgccggct ttccaacctc gggggttcaa
     9361 tctttgtcgg agaggatctg gtctacttcc tctttcatgt gatccaggtg cgtgccgggc
     9421 caatagacgc gacagcagcg cggacaaaga taaaaggaat tataataccg gtaaatgaaa
     9481 tccggcaccc ttccctccac ctctccttta gtcactggtt gaagctcttc attgcacatc
     9541 agacaacggg aaaaatactg gttatccgac ggtttcaggt ggagtttttg gagtacttcg
     9601 cgcacctgct cccggggatc gttggcttcc aggatggtga cctggaggtc tttgggcgcc
     9661 gccgaaatcc gccggctccg agtcaggaac attcggccct caatcacggc cacccgggca
     9721 gcctcttgga ttgggccgcc tccccaggaa gccgcatcaa accccaaggc ccggagcttt
     9781 ttggccaatt tccctgtggt gcggtcggcc aagaacttca tatttttatt ttattctttt
     9841 cgcctgggag agtcaaagcc cccgttcccc ttacccacct ccacttttgc gatcctcttt
     9901 tctgttgaga aacaagcttg tggtccggca atgcctgaga ttcaatcgat ctctattctg
     9961 cttggcgaaa agtcaagacg ggcaaggcca gtttcagat
//
LOCUS       DASPRR010000501         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2374335, whole genome shotgun sequence.
ACCESSION   DASPRR010000501 DASPRR010000000
VERSION     DASPRR010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374335"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VEL68_18480"
     CDS             <1..1006
                     /locus_tag="VEL68_18480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXZ38018.1"
                     /translation="SVTQRVPYPGKRGLRSEMSQKEAEAALSDYEEAKNKVSREMKMA
                     YFDLFYANKAIEVTERNREVLKLLNQIAETRYSVGEGIHTDVLKAQVELSKMIDELIM
                     LNQSKRALKARINTLLHQPPFAPLGDPAEVMPEKFSADPEELVKAAAQNRPLLQSMKR
                     TVERNRAGLRLAEKDYYPDFDFKLAYGQRDDGPNGRRSDMITAMVGINLPLWYKSKQE
                     RKVAESHKDIQSAKDQLAAMTNEIRFMISDKMTEIERAEKQVDLLKTGIIPQATLSLD
                     SAMSSYRVSKVDFMTVLDSLMTLFKYEIQYYRLLTDSRKSIAEIETAVGKSSVEEKKG
                     "
     gene            1003..2256
                     /locus_tag="VEL68_18485"
     CDS             1003..2256
                     /locus_tag="VEL68_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ38019.1"
                     /translation="MRRFLSFLVMTLFILALAGCSEQREKIADLFRWGEKEMKHEIKK
                     VDPKAAEELDMSKMTLETLKKQGLKMTEIPSEAVFVSPERQQVSGVRFGRAEYRNLDR
                     VIRTVGRIEFDEKKISMVNPKIGGWIEELYVDYTGKMVRKGQPLLSIYSPDLVAAQEE
                     VLLALKAKKILGASPIAEVAEGGDRLLQGARRRLLLWDITPKQLETLEQTGEIKKSMV
                     LYSPADGFVLEKMAYKGMALMPGAALYKIGDLSSIWVIGDIYEYELPFVKIGDKAQIT
                     LAYFPGETFEGTATYIYPMLDPKTRTAKVRFDLPNPEFKLKPEMWANVELKIALGRKL
                     VIPEDAVMDSGTMQMVFVDRGQGYFESRHIQVGSKVQGYYEVLSGLKEGEKVVTSANF
                     LIDSESRLSGAMGGMAGMEGMGHQH"
     gene            2340..>4731
                     /locus_tag="VEL68_18490"
     CDS             2340..>4731
                     /locus_tag="VEL68_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXZ38020.1"
                     /translation="MITKLIEYSARNKFIIFIFTFFAIAAGIWALWNTPLDAIPDLSD
                     VQVIVYTEWAERSPTLIEDQVTYPIVTALLSTPYVKVVRGFSFFGVSFVYVIFEDGTD
                     IYWARTRVLEYMQGIQGKLPPGVTPTLGPDATGVGWGFEYALVDTTGKYDLSQLRTLQ
                     DWYVRYWLQSVPGVAEVASIGGFVKQYQIHINPDTLNAYKMPIMTVAENIRKGNRDTG
                     ARVLEFTGREYMVRGRGYIQSIPDIEKIVVGTDERGTPILVKNIGHVTLGPDIRRGST
                     ELDGTGEVVGGIVVVRFGVNSHEVIQRVKQKIKEVTPSLPQGVKIVTTYDRSDLIERS
                     ITTLKEKLIEESIIVSLVIVLFLFHFRSALVPILTLPIAILLSFLAMYGIKLGSNIMS
                     LGGIAIAIGAMVDAAIIMVENAHTRLEEWELMGKPTPRNEVLIQAAKEVGRPLFFSLL
                     IITVSFLPIFTLQEQEGRLFKPLAFTKTFAMFFAAFLSVTLAPVLMTLLIRGKILPEE
                     KNPINRFLIWVYQPVVHFALRWKKSILIAAILVMAATIPVFLKLGSEFMPPLYEGTLL
                     YMPTTLPGASIGTANQIMQVQDKIIKSFPEVESVFGKAGRANSATDPAPLEMIETVIN
                     LKPEKDWRPGMTPEKLINELDKAVRMPGVSNAWTMPIKARTDMLSTGIRTPVGIKVLG
                     PKLEEIQRIGEHLEMVLKDVPGTRNIFAERVTGGYYLDFTIRREEIARYGLTVEDVEA
                     VLETAIGGMTITTTVEGRERFPINIRYYRDFRSDLGPLKRVLVPTMGGAQIPLGQLV"
BASE COUNT         1134 a         1229 c         1367 g         1001 t
ORIGIN      
        1 ttcggtcacc cagcgggtgc cttacccggg gaagagaggc cttcgcagcg agatgtccca
       61 gaaggaagcc gaggcggcgc tcagcgatta tgaggaggcc aaaaacaaag tgtcccggga
      121 gatgaagatg gcctacttcg acctcttcta cgccaacaag gcgatcgaag taacggaaag
      181 gaaccgggag gtactgaaac tcttgaacca gatcgccgag acccggtact ccgttggaga
      241 gggaattcat accgacgttt tgaaggccca ggtggaactt tccaagatga tcgacgagct
      301 gatcatgctc aaccagagta aaagggcctt aaaggccagg atcaacaccc ttctccatca
      361 gcctcccttc gcccccctcg gcgaccctgc ggaagtcatg cccgaaaaat tctccgccga
      421 tccggaggaa ctggtgaaag ccgccgccca gaatcgcccc ctgctccagt ccatgaaaag
      481 aacggttgag cggaaccggg ccgggctgcg gctagcggaa aaggattatt atccggactt
      541 cgatttcaag ctggcctacg ggcagaggga cgacggcccg aatggccgga ggtcggacat
      601 gatcacggct atggtgggca tcaaccttcc cctttggtat aagagcaagc aggaacgaaa
      661 agtcgctgaa tcccataagg acatccagtc ggcaaaggac cagctcgccg ccatgaccaa
      721 tgaaatccgc tttatgatca gcgataaaat gaccgagatc gaaagggccg agaaacaggt
      781 cgatcttttg aaaacgggga tcatccccca ggcgaccctt tccctggatt cagctatgag
      841 ctcgtaccgg gtgagcaaag tggatttcat gaccgtcctg gacagcctga tgacgctgtt
      901 caaatatgag atccaatatt accgcctgct aaccgattcg agaaagagca tcgccgagat
      961 cgagaccgct gtgggtaagt catccgtaga ggagaagaag ggatgagacg gttcctgagc
     1021 tttttggtca tgaccctgtt catcttggcc ctggcgggct gcagcgagca aagggagaag
     1081 atcgccgatc ttttccgttg gggcgagaaa gagatgaagc atgaaattaa aaaggtggac
     1141 cccaaggccg cggaagaatt ggacatgagc aagatgacct tggaaacctt gaaaaagcaa
     1201 gggctgaaga tgaccgagat cccctccgag gccgttttcg tatcgccgga gcgtcagcag
     1261 gtgagcgggg ttcgcttcgg ccgggcggaa tatcgaaacc tggatcgggt catccggaca
     1321 gtgggccgga tagaatttga cgagaaaaag atatccatgg tcaatccaaa aatcgggggc
     1381 tggatcgagg aactctacgt ggattacacc ggaaagatgg tccggaaggg gcagccctta
     1441 ctctctattt acagccccga cctggtggcc gcgcaggaag aagtgctgct ggccctgaag
     1501 gccaagaaaa ttctcggcgc cagccccatc gccgaagtcg cggaaggggg ggatagactc
     1561 ctccagggag cacgcagacg gctgctttta tgggatatca cccctaaaca gttggagacc
     1621 ctggagcaaa ccggggagat caagaagagc atggtcctct attccccagc ggatggtttt
     1681 gttttggaga agatggctta caagggaatg gcgctcatgc cgggcgcggc cctatacaag
     1741 atcggagacc tgtcttccat ctgggtcatc ggcgacattt atgaatatga attgcccttc
     1801 gtcaagatcg gagataaggc ccagatcact ctggcctatt ttccggggga gaccttcgaa
     1861 gggacggcca cgtacatcta tcccatgctg gacccgaaga cccgaacggc caaggtccgt
     1921 ttcgaccttc ccaacccgga gttcaaacta aagccggaga tgtgggccaa tgtggagttg
     1981 aagatagccc tggggaggaa gctggttatc ccggaggatg cggtcatgga ttcggggacc
     2041 atgcagatgg tctttgtgga tcgggggcag ggatacttcg aatcccggca tattcaggtg
     2101 ggctcgaaag tgcaagggta ttacgaagtt ttgtccggat tgaaagaagg ggaaaaggta
     2161 gttacttccg ccaatttcct catcgactcc gagagccgcc tctccggagc catggggggg
     2221 atggcgggca tggaaggtat gggacatcaa cattagagat acagttcgga gttagaagaa
     2281 aggtcgttgg tttttagcta tagacactcc gaactctagg cactctagac actcttctta
     2341 tgatcaccaa actcattgaa tatagcgcca ggaataaatt tattatcttc atctttacct
     2401 tttttgcgat cgccgccggg atttgggccc tttggaatac tcccctggat gcgattccgg
     2461 acctctccga cgtccaggtg atcgtctaca cggaatgggc cgagcgaagc ccgaccctga
     2521 tcgaagacca ggttacctat cccatcgtca ccgctcttct ttccactccc tatgtgaaag
     2581 tggtgcgggg gttctccttt tttggcgttt ccttcgttta tgtaattttc gaggatggaa
     2641 cggatattta ctgggccagg acccgggtcc tggaatatat gcaaggcatc cagggaaagc
     2701 ttccgccggg cgtcacccct accctgggcc cggacgctac gggcgtaggc tgggggttcg
     2761 aatacgccct ggtggacacg acggggaaat atgacctctc tcaactgcga accctgcagg
     2821 attggtacgt gcgttactgg ctccaatccg taccgggcgt ggcggaggtg gcttccatcg
     2881 gcgggttcgt aaagcagtat cagattcata tcaatcccga taccctcaac gcctacaaga
     2941 tgccgatcat gacggttgcg gaaaacatcc gcaagggaaa tcgggatacg ggggcccggg
     3001 ttcttgaatt caccggccgg gaatacatgg tccgggggcg tgggtacatc cagagcatcc
     3061 cggacataga aaagattgtg gtgggaacgg atgagcgggg tacgccgatc ctggtaaaaa
     3121 atatcggcca tgtcacgctg ggcccggata ttcgtcgcgg ttcgaccgaa ctggatggga
     3181 ccggcgaggt cgtgggcggg atcgtggtgg tccgtttcgg ggtcaactcc catgaagtta
     3241 tccagcgggt gaaacagaag atcaaagaag taaccccttc cttgccccaa ggcgtaaaga
     3301 ttgtgaccac ctatgatcgt tctgatttga tcgagcgctc cattaccacg ctgaaggaaa
     3361 agctcattga ggagtcgatc atcgtcagtt tagtgatcgt tctgttcctt ttccattttc
     3421 ggagcgcact ggtacccatt ctcaccctgc ccattgccat ccttctgtcc ttcctggcca
     3481 tgtacggcat caaactgggc tcaaacatca tgtccttagg gggaattgcc atcgccatcg
     3541 gtgccatggt ggacgcagcc attatcatgg tggagaacgc tcacacgcgt ctggaagagt
     3601 gggaactgat ggggaaaccc actcctcgaa acgaggtgtt gattcaggcg gccaaagaag
     3661 tggggaggcc cctgttcttc tccctgctga tcatcaccgt ctctttcctc cccattttca
     3721 ccctccaaga gcaggaagga aggctgttta agcctctggc cttcaccaag acattcgcga
     3781 tgttcttcgc cgcttttctc tccgtaaccc tggcgccggt tcttatgacg ctcctgatcc
     3841 gggggaagat cctgcctgag gagaagaacc cgatcaatcg ttttctgatc tgggtttatc
     3901 agccggttgt ccatttcgct ctgcgatgga aaaaaagcat tttgattgcc gccatcctgg
     3961 tcatggcggc gacgatcccg gtattcctga agctcgggtc agagttcatg cctcctctct
     4021 atgagggaac cctcctttac atgcccacaa ctcttccggg ggcttccatc ggcacggcca
     4081 atcagattat gcaggttcaa gataaaatca tcaagtcctt ccccgaggtg gaatcggtct
     4141 ttggcaaagc agggcgggcc aacagcgcga ctgatcccgc ccccctggag atgatcgaga
     4201 ccgtgatcaa cctcaagccg gagaaagact ggcggccagg gatgaccccg gagaaactca
     4261 taaacgagct ggataaagca gtgaggatgc ccggtgtctc caatgcctgg accatgccca
     4321 tcaaggcccg aactgatatg ctgtccacgg gaatccggac cccggtaggg atcaaagttt
     4381 tggggccgaa gctggaagaa atccagcgga tcggggaaca tttggaaatg gtcctcaaag
     4441 atgtgcctgg aacgagaaat atttttgctg aaagggtaac cggggggtat tacctggatt
     4501 tcactatccg ccgggaggag atcgcccgct atgggctaac agtggaagac gtggaagcgg
     4561 tcctggagac agctatcgga gggatgacca taaccaccac cgtggagggg cgcgagcgct
     4621 tcccgataaa catccggtat taccgggatt ttcgaagtga cctgggacct ttgaaacggg
     4681 tcctggtccc aaccatgggc ggagcccaaa ttcccctggg gcaattggtg g
//
LOCUS       DASPRR010000502         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_775848, whole genome shotgun sequence.
ACCESSION   DASPRR010000502 DASPRR010000000
VERSION     DASPRR010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775848"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VEL68_18495"
     CDS             complement(<1..191)
                     /locus_tag="VEL68_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HXZ38021.1"
                     /translation="MKKVLLDGERLSLEDVQEVAEGGAGVKIHPSAGEKVKRSRDFVE
                     KTLRQGEKIYGVTTGFGLL"
     gene            complement(271..483)
                     /locus_tag="VEL68_18500"
     CDS             complement(271..483)
                     /locus_tag="VEL68_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38022.1"
                     /translation="MKKVEAFVKSHKLAGVAMALQKMKSFSRNEALDALALRETPEIQ
                     STLRLHGEKRRDPSGLEVLFLLFPLC"
     gene            complement(499..3603)
                     /locus_tag="VEL68_18505"
     CDS             complement(499..3603)
                     /locus_tag="VEL68_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXZ38023.1"
                     /translation="MQGIIHFVLRNRLLMGIFGFLLLAGGYYSYTRLPVDAFPDVTPV
                     LVQVFTETQGLAPEEVEKYITYPTEVAMNGLPNLKELRSTSNFGLSVVNIYFHDRTDI
                     YFARQLVNERLQRARENIPEGFGRPVMGPIATGMGQVLFYFLEDQSGMRSPEEMREIQ
                     DWLIKFNVQTVPGVTEVLSLGGEVKQFQVRVRPRDLLRFDLSLKEVLQAVKENNANVG
                     AQFIVKNNAEYIVRSVGLAEKITDLESIVVKTEKGTPVFLSQVADVAIGGEVRRGLAT
                     MDGKGEAVVGMVLKLIGMNTSEVIGGVKAKLEEINKILPPGVRIIPYYDQATLVKKCI
                     QTVTRALLEAGILVVLIQLIMLGGIRPSVVVLAAIPFSLALAFLFMNYFGISANLMSL
                     GGLAIALGLLVDGSVVMVENIDRRLREADREEPLVHVVAQACIEVARPIIFSLLIIVI
                     VFLPLFTLQGVEGKTFRPLAQTMSLAMFGSLIFAVLLVPVLGSLLMKRPRDMTLGHQG
                     VFALRALLGVYRPAVTFFVYHPKMAILLAVTLLLIGGTLFTRLGSEFIPRLQEGDLLV
                     RATMAPSISLDEARETMLRFERRIMSQYPEVARVVTRIGRGEVGAHADPVNSAEAFIA
                     LKPRESWKKAKTSEELIGKMSEAFEKFPGVQFNFTQPIAAAVDELITGIKGDLAVKIF
                     GPDMEVLKNKAEEVEAILQKVPGAADVLKDQITGTPQLRIRVDRLAISRYGVNVQDVQ
                     DLIQTAVGGTAAGQLFEGIKRFDITVRYSPESRVTAEAIARILVPAPGGLRIPLADFA
                     KIEEIVGPRQITREKNQRFITVQCNVHQRDIGSFVQEAQREIDSRLKLPSGYLITWGG
                     QFQLQQEANRRMALVIPATLTVVFLLLYSTFNSIKNCLLILMNIPLALVGGVVGMWMT
                     GQNLSVPASVGFIALFGIALANGMVLVTYLNQLMREGIPVVEASIRGACLRLRPVLMT
                     ATAAALGLVPVVLSGGTGSEVQRPLATVMIGGLVTSTLLTLLVIPALYRWFAINPEED
                     AVMK"
     gene            3602..>3786
                     /locus_tag="VEL68_18510"
     CDS             3602..>3786
                     /locus_tag="VEL68_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38024.1"
                     /translation="MLHRLSVGVPVAKSMPGQLCLQSESPLGDVSLAGLNPRYDFDMG
                     LVAAADCHGSGFEPVLGL"
BASE COUNT          835 a         1109 c         1016 g          826 t
ORIGIN      
        1 caaagaagtc caaatccggt ggtcaccccg taaattttct ccccctgacg gagagtcttt
       61 tccacaaaat ccctggaccg tttcaccttc tcgccagccg aagggtgaat ctttaccccg
      121 gctccccctt cagccacctc ctggacatct tccaggctta atctttctcc gtcgagaagt
      181 actttcttca cgtgctctct tcctttttat gaatggccat aaatttattc ccttggtgtg
      241 gccgacctcg gcatacaccg atagttcatt ttagcagagg ggaaaaagga gaaacaagac
      301 ctccaggccg cttgggtccc ttcgtttttc cccatgtagg cgcaaggtgg attgaatttc
      361 aggtgtttcc cgcaatgcaa gggcatcgag ggcctcattc ctgctgaagc tcttcatctt
      421 ttggagggcc atggcgaccc ccgccaattt atgggatttg acaaacgctt caactttttt
      481 catcgaacaa ccctcttttc atttcatcac ggcatcttct tccgggttga tggcaaacca
      541 cctgtagaga gcgggtatga ctaacaaggt taacagggta gaggtgacca ggcccccgat
      601 cattacggtc gccagggggc gctgcacttc gctccctgtc cccccggaaa gcaccaccgg
      661 aacgagcccc agggccgcag cagtcgccgt catcaaaacg ggccgcagcc tcaagcaggc
      721 ccctcgaata ctagcctcga ccacggggat gccctcccgc atgagctggt tcaaataggt
      781 caccagaacc atgccgttgg ccagagcgat cccgaaaaga gcgatgaatc ccactgatgc
      841 aggaacggag agattttgac cggtcatcca catgccgacg accccaccta cgagtgccag
      901 tggaatattc ataagaatca ataaacaatt ctttatggag ttgaaggtgg aatacaacag
      961 caggaagaca acggtgaggg tggccggaat caccagggcc atgcgccggt ttgcttcctg
     1021 ctgcagctga aattgaccgc cccaggtaat cagatacccg gaggggagtt tgagccgtga
     1081 atcgatctcc ctttgggcct cttgaacgaa ggacccgata tccctttggt ggacgttgca
     1141 ctggaccgta atgaacctct ggttcttttc ccgggtgatc tgccgggggc cgacgatctc
     1201 ctcgattttg gcgaagtcag cgagcgggat ccgtaatcct cctggcgcag ggacaaggat
     1261 acgggcgatg gcttccgctg tcaccctgct ttccggggag tagcggacag tgatatcgaa
     1321 tcgcttaatt ccttcaaata attgacccgc agcggttcct ccgacggccg tttggatcaa
     1381 atcctgaacg tcctggacat tgaccccata gcgggaaatc gccaggcggt ctacccgaat
     1441 ccgcaactga ggggttcccg taatttgatc tttgagtacg tcggctgcgc ccggaacctt
     1501 ctggagaatg gcctcaacct cctccgcttt gttcttcagc acttccatat ccgggccaaa
     1561 gatctttacg gccaggtcgc ctttgatccc cgtgatcagt tcatccacgg cggcggcgat
     1621 cggctgggtg aaattgaatt gcactccggg aaatttttca aaagcctcgc tcattttgcc
     1681 gatcagttcc tccgaggtct tggctttttt ccaggattcc ctgggcttca gggcaatgaa
     1741 tgcctcggcg ctgttcaccg ggtccgcgtg cgcccctacc tcccctcgtc cgatgcgcgt
     1801 taccaccctg gcgacttcgg ggtattgaga catgattcgc cgttcgaaac gcaacatggt
     1861 ctcccgggct tcgtcgaggg agatggacgg agccatggtg gctcggacca gcaaatcgcc
     1921 ctcctggagc cggggaatga attccgagcc cagccgggtg aacagagttc caccgatcag
     1981 aagaagcgta acggcgagta gaatcgccat tttgggatgg tacacaaaaa aggtaaccgc
     2041 cggccggtaa accccgagga gggcccgcag ggcgaatact ccttgatgtc ccaaagtcat
     2101 atctctcggc cttttcatca acaggctgcc cagcaccggg accaaaagga ccgcaaagat
     2161 cagggaccca aacatggcta aggacatggt ctgagcgagg ggacgaaaag tctttccttc
     2221 gactccctga agagtaaaca gggggagaaa cacgatgacg ataatcaaca gggaaaagat
     2281 gatcggccgg gccacttcga tacatgcttg agcgacaaca tgaaccaggg gttcttcccg
     2341 gtctgcttcc cgcaggcgcc ggtcgatatt ctccaccatc accactgatc cgtccactag
     2401 aagacccagg gcgatggcca ggccgcccag ggacatcagg ttggcggaga tcccgaaata
     2461 attcatgaac agaaaggcga gagccaggga aaagggaatt gcggccagaa cgaccacgct
     2521 cgggcgaatg ccgcccagca tgatcaattg gatcaaaacg acgagaattc ccgcttccag
     2581 gagcgctcga gtgacggtct ggatgcactt tttcacgagt gtcgcctgat cgtagtaggg
     2641 gatgatcctg accccggggg ggaggatttt gttgatctcc tccaacttgg cttttacccc
     2701 gccgatcacc tcggaagtat tcatgccgat cagcttcaag accatgccga ccacggcttc
     2761 gcccttgccg tccatggtgg ccaacccgcg gcggacctcc ccgccgatgg ccacgtcggc
     2821 gacttggctc aggaaaacgg gggtcccttt ctccgtctta accacgatgg attcaaggtc
     2881 ggtgatcttc tccgccagtc cgacagaacg cacgatgtat tccgcgttgt tctttacgat
     2941 gaactgcgca cccacattgg cattgttctc tttcacggcc tgcagaactt ccttcagcga
     3001 cagatcgaag cgaagcaggt cgcgtgggcg gaccctgact tggaattgtt tgacctcccc
     3061 tccgagggaa aggacctcgg tcacccccgg cacggtctgc acattgaact taatcagcca
     3121 gtcctggatc tcccgcattt cttcggggct ccgcattcca ctttgatctt caagaaaata
     3181 gaacagaacc tgccccatgc ccgtcgcgat gggccccatc accggccggc cgaagccctc
     3241 ggggatgttc tcccgggcgc gctgcagtcg ttcattcacc agttgccggg caaaatagat
     3301 gtcggtcctg tcgtggaaat agatattcac gacggatagc ccgaagttgg aggtggaacg
     3361 gagttccttc aggttgggga ggccgttcat ggcgacttcg gttggatagg ttatgtattt
     3421 ctcgacttcc tccggggcca agccctgcgt ctccgtgaaa acctggacca ggaccggggt
     3481 gacatcgggg aaggcatcca ctggaagcct ggtatagcta taatatcccc ctgccaagag
     3541 aagaaaacca aagatcccca tcaggagtcg attccgtaat acgaagtgaa tgatgccttg
     3601 catgttgcat cgcctttcag tgggcgtgcc cgtcgccaaa tccatgccgg gacaactgtg
     3661 cctgcagagt gaatcccccc ttggagacgt atcgctggcc ggccttaatc cccgatacga
     3721 cttcgacatg ggtctcgtcg ctgcggccga ctgccacggg tcggggttcg aacccgtcct
     3781 cggtct
//
LOCUS       DASPRR010000503         6686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_775885, whole genome shotgun sequence.
ACCESSION   DASPRR010000503 DASPRR010000000
VERSION     DASPRR010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775885"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VEL68_18515"
     CDS             <1..865
                     /locus_tag="VEL68_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38025.1"
                     /translation="VGYTGQISLGHAGFFAIGAFTTVMFVGQMEFPLFIALPLGALIS
                     AGAGFILGLPSLRLEGPYLAIATMGFGMAVTTIIKHMEFFGGRMGLQAPKLYFFGTPM
                     KDIHFYYVICAVGAILTVAAVKLFKTKVGRAFIAIRDSDVAAETMGVNLNYYKTLSFA
                     VSAFYTGIAGGLYAFILGFINPEGFHIIMSITFLAMVVIGGVGSIMGSIAGAILMTYL
                     DVRLQAIQDIPGIGALLVSFSEQYMSMGGISNVAIIVYGLIMVLIILFEPLGIFGIWI
                     RTKKYWKTYPF"
     gene            967..1839
                     /locus_tag="VEL68_18520"
     CDS             967..1839
                     /locus_tag="VEL68_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38026.1"
                     /translation="MLGQLIVSGLAVGFCYALLALAMVIIYKTSDVLNFAQGEMAMIS
                     SFIAFVLLDSYQISFPIALALTLLFAAALGALLEFAFLRPAKNPTVIGMIIITLGFEM
                     ILMGIAGWKWGPDQRSLPFPIPSFETYNFAGLVVSKINFWTIITCMVLIVILFLFFQY
                     SKLGTAMRATQQNRLVARLMGIRTKRIFSFTWALSSLIGAVSGMFIAALTVLDPPMMM
                     DPLMKAFASAVLGGMTSLPGAAVGGAILGVVENLFGGYISLPFKSVVAFAVIVLMLCI
                     RPSGLLGKHFVKKV"
     gene            1943..3127
                     /locus_tag="VEL68_18525"
     CDS             1943..3127
                     /locus_tag="VEL68_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38027.1"
                     /translation="MKKSILLLSASLLVFAVLCIPTWGGAQAVRGVTDTEVLVGQTGP
                     QTGPAALWGAVARGTGLYFKGINDEGGIHGRKIKYFLRDDSYQPAKTKAIGKEFVEQI
                     GIFSTVGCVGVGPGMSVRDFYSENKVVMVSMGCSGVTNWIKPLQKYIFPIYPLYIDEA
                     FALTRYFHETMKLTKIAMFYQNDDYGKQGLEGVEHYVKAKNLQMVATVSAEVTDRDLS
                     SHALKLKDSGAQAVIMWAMPTHGALLLGECAKIQYKPKWGTSSTLSDGVAMISISKGL
                     WAGMISSNFGELPDSNHPLMVKYRSWHQKYEPKERWSTFYIAGITFAEPFVEGLRRAG
                     KNLTPETMVKAMESMKDWQGIGPPITFTPTDHQGGKHIFFMEVLPDGNAKRLTDWMRI
                     TY"
     gene            3503..4681
                     /locus_tag="VEL68_18530"
     CDS             3503..4681
                     /locus_tag="VEL68_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38028.1"
                     /translation="MKRGPIIFVALLILIGCSFPTSIPAQTVRGVTDTEILIGQWGPE
                     TGPAAPWGAVARGTGLFFKGINDEGGIHGRKIRYFLRDDAYQPAKTKAIGKEFLEEIG
                     VFAVVGGVGVAGGMTNRDALMEKKVSWVGPATGVRNWIEPFQRYLFAVYPLFEDEAYA
                     LASFLHDKMGFKKVGVFYQNDDYGKQGMNGVEKYFKEKNVELVAKVTGEVTERDLSTH
                     AMKLKNSGAEAVVIWTNPTHSVLILAESAKIGFKPQWATTNGLADGPFMLQLSKGLWA
                     GMINSQFLEPIESNHPLLVKYRSWWQKYEPKERWGINYAAGILFAEPLVEGLRRAGKN
                     LTPDSFVSAMETLKDWRGIGLPVTFSPAERQGGKHVYFSQVLPDASYKKLSDWILITK
                     "
     gene            4908..5678
                     /locus_tag="VEL68_18535"
     CDS             4908..5678
                     /locus_tag="VEL68_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38029.1"
                     /translation="MAILTVEDLSISFGGLKALNGVNFSVEKGEIYSIIGPNGAGKTT
                     VFNCISGIYKPTGGKILFKDQDITPFKPHQVAQVGIARTFQNIELFAHMTTMDNLMLG
                     RHTKLKTGLWAGATFLTPNSRAAREEIQNREKVEDIIDFLELQSSRNQMVMNLPYGKQ
                     KLVELARALALEPEVLLLDEPSAGMNLEEKEDLIFSIRDIRDDFGITILLVEHDMNLV
                     MDISDRILALNYGMVLAEGVPQMIQTHPEVLKAYLGEG"
     gene            5683..6462
                     /locus_tag="VEL68_18540"
     CDS             5683..6462
                     /locus_tag="VEL68_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38030.1"
                     /translation="MGSILQVKNIETLYYDIIKALRGISLDIQEGTISVVLGGNGAGK
                     TTTLKTIMGLLEDQPDKGTIEFMGRRIDGMDAEDVVNLGIAMVPEGREVFPELTVLEN
                     LKMGAYIRKDKKGVQEDQDRVIRYFPILKERASQQAGYLSGGEQQMLAMGRALMTRPK
                     LLMLDEPSLGLSPILVREIFEIIKTINKEGTTILLVEQNAKMALSVAHFGYVLETGRI
                     VLANTPQNLMEDEDVKEFYLGIKSEVSGKGYQRWRRKKRWR"
BASE COUNT         1572 a         1712 c         1940 g         1462 t
ORIGIN      
        1 cgtgggatac accggccaga tctccctggg ccatgcgggt tttttcgcca tcggagcctt
       61 taccacggtc atgtttgtcg gccagatgga atttccctta ttcatcgctc tgcccctggg
      121 ggctctcatt tccgccgggg cggggttcat cctgggcctc ccctcgctgc gcctggaagg
      181 cccctacctg gcgattgcca ctatgggttt cggcatggcc gtcaccacga tcatcaaaca
      241 catggaattc ttcggcggga ggatggggct gcaggccccc aagctgtact tcttcgggac
      301 tcccatgaag gacatccatt tctactacgt gatttgcgcc gtcggagcga tcttaaccgt
      361 cgcggcggtg aaattgttca agaccaaagt ggggcgggcc ttcatcgcca tccgcgacag
      421 cgacgtggct gccgaaacga tgggggtgaa ccttaactat tataaaaccc tttcctttgc
      481 cgtcagtgct ttctataccg gcatcgctgg cgggctctac gcctttatcc tgggattcat
      541 caacccggaa ggattccata tcatcatgtc cattaccttt ctggccatgg tggtgatcgg
      601 gggggtaggc tccatcatgg gctccattgc cggggccatc cttatgactt acctggacgt
      661 caggttacaa gccatccaag acatcccagg catcggggcg ttgctggtga gtttttccga
      721 gcagtatatg agcatgggcg gcatttccaa tgtggccatc atcgtttacg ggctgatcat
      781 ggtcctgatc atcctcttcg agcccctggg tattttcggc atctggattc ggaccaagaa
      841 gtactggaag acgtatccgt tttaaagaca gtgtcagtgt cagtggacag tgtcagtagc
      901 atcgataaaa atctttgttt tcctgacact aatcactgac tctgacactg gctgttagga
      961 ggcggaatgc tcggacagct tattgtcagc ggcctggccg tgggtttttg ctacgccctt
     1021 ctggctctgg ccatggtcat catttacaag acctcggatg tgttgaactt tgcccagggg
     1081 gagatggcca tgatcagttc cttcattgcc ttcgtactcc tggacagcta tcagatttcc
     1141 ttccctatcg ccctagcttt gacgcttctc tttgccgccg cgctgggtgc cctcctggag
     1201 ttcgcctttc tccggccggc caagaatccc acggtgatcg ggatgatcat tatcaccctg
     1261 ggattcgaga tgatccttat gggaatcgct ggatggaagt gggggccgga ccagcgctcc
     1321 ctgccgttcc ccattcccag cttcgagaca tacaacttcg ctggcctggt ggtgagcaag
     1381 atcaacttct ggaccatcat cacctgcatg gtcctcatcg ttatcctctt cctcttcttc
     1441 cagtattcca agttgggaac ggccatgcgg gccacccagc agaacaggct ggtcgcccgg
     1501 cttatgggca tccgcaccaa acggatcttt tccttcacct gggcgctgag ttccttgatc
     1561 ggggcggtgt cgggaatgtt tatcgccgcc ctcaccgtcc tagaccctcc catgatgatg
     1621 gatcccctga tgaaagcctt cgcctctgcc gttctggggg gcatgaccag cctgccggga
     1681 gcggccgtgg ggggagcgat cctcggggtg gtggaaaacc tgtttggggg atatatctcc
     1741 ttgcccttca aatcggttgt ggcctttgcg gtcatcgtgc tgatgctctg catacggccc
     1801 agcgggctgt tgggaaaaca ttttgtgaag aaggtttaat agcgaataga gaaacggaag
     1861 gaggtggtcg ggggaaagaa ggggtgaatg tggccgagag gaaataaccc gaggcggaaa
     1921 aactcaaaag gagggaggga gcatgaaaaa gagcattctt cttctttcag ccagtctatt
     1981 ggtttttgca gtgttgtgca ttcccacctg ggggggagcg caggcggttc gaggggtgac
     2041 cgatacggag gtcctcgtgg gtcaaaccgg tcctcagaca gggcctgccg ctctctgggg
     2101 cgccgtcgcc cggggaacag ggctgtattt caaggggatc aatgatgagg gcgggattca
     2161 tggccggaag atcaaatatt tcctgcgcga cgactcctac cagccggcca agaccaaggc
     2221 catcggcaag gagttcgtgg agcagatcgg aatcttttcc acggtagggt gcgtgggggt
     2281 cgggccgggg atgagcgtcc gggattttta ctcggaaaac aaagtcgtca tggtatccat
     2341 gggatgcagc ggggtgacca actggatcaa gcctctccaa aaatacatct ttcccattta
     2401 ccctctatac atcgacgagg cctttgccct gacccggtat ttccatgaaa ccatgaaatt
     2461 gaccaaaatc gccatgttct accagaatga cgactatgga aaacaaggcc tggagggtgt
     2521 ggaacactat gtaaaagcga agaacctgca aatggtggcc acggtctcag cggaggtgac
     2581 ggaccgggat ttaagttctc acgcactaaa attgaaggat tctggagccc aggcagtgat
     2641 catgtgggcg atgcccaccc atggagccct gcttttgggg gaatgcgcca aaatccagta
     2701 caaacccaaa tgggggacca gcagcaccct ctccgacgga gtggcgatga tttcaatctc
     2761 caaaggcctc tgggctggaa tgatcagttc caattttgga gaactcccgg attcgaatca
     2821 tcccctgatg gtcaaatacc gctcctggca tcaaaaatat gagcccaagg agcgatggtc
     2881 gaccttctat attgccggaa ttacttttgc tgaacccttc gtcgaaggat tgaggcgcgc
     2941 ggggaaaaat ttaacgcccg agacgatggt caaagcgatg gaaagtatga aagactggca
     3001 agggattgga ccgcccatca cctttacccc aacagaccac cagggcggga agcatatttt
     3061 cttcatggag gttctgccgg acggaaatgc caagagactg acggactgga tgcggattac
     3121 ctactaatag gggatgcgag ggaaagaaag acgcggggac agaagcggag aaacggagat
     3181 aaaacggaaa aactctgatg atctcgtaag aagtttcttg aagcgtcacc ccggcgaaag
     3241 ccggggtcca gacatcgtcc agacgaaagt cgggaaccat gtcaaagact ggatcccagt
     3301 tttcaccggg gaccctggat tccggctttc gccggaatga caaattttat gacatttcaa
     3361 ctttctacga gttcatcaac tctgattgtt tgaattgagg gtataggaaa gaggggcgat
     3421 aggacagttg ttcggaggat catccatcgc ccctgcgggt atttggacgg catgagcttc
     3481 aggtcaaaca aggggaggtc gtatgaaaag gggccctatc atatttgtgg ctttattgat
     3541 tctgatcggc tgcagttttc ctaccagtat cccagctcag accgtaaggg gagtcaccga
     3601 tacagagata ttaatcggtc agtgggggcc tgagaccggg ccggccgctc cttggggagc
     3661 cgtagcccgg ggaacgggcc tcttcttcaa ggggatcaat gatgagggcg ggattcatgg
     3721 ccggaagatc agatatttcc tgcgcgacga tgcctaccag ccggccaaga ccaaggctat
     3781 cggcaaggag ttcctggagg agatcggggt tttcgcggtg gttggcggag tgggagttgc
     3841 cggggggatg accaaccgag acgccctcat ggaaaagaag gtcagttggg ttggacccgc
     3901 gaccggagtt cgaaactgga tcgagccatt tcaaagatac ctctttgcgg tttatccgct
     3961 ctttgaggac gaggcctatg ccctggcgag tttcctgcac gacaaaatgg gattcaaaaa
     4021 agtgggggtc ttctaccaga atgacgatta tggaaagcag gggatgaacg gggtggagaa
     4081 gtacttcaaa gagaaaaacg tggagctcgt ggccaaggtt acgggtgagg taacagaaag
     4141 ggacctctcc acccatgcca tgaagctgaa gaattcgggg gcggaagccg tcgtcatttg
     4201 gaccaacccc acccacagcg tcctaattct ggccgagtcg gctaaaatcg gttttaaacc
     4261 ccagtgggcc acgaccaacg gcctggccga cgggcccttc atgcttcaat tatccaaagg
     4321 cctctgggcg ggaatgatca actcccagtt cctggaaccc atcgaatcga atcatccctt
     4381 gctggtgaag taccggtcct ggtggcagaa gtatgaaccc aaggaacggt gggggatcaa
     4441 ttacgcggcc gggattctct tcgccgagcc gctggtggaa ggactccggc gagcgggaaa
     4501 aaacctgacc ccggattcct ttgtttccgc catggaaacc ctcaaggact ggcgggggat
     4561 cggattgccg gtgaccttct cccccgcgga aaggcagggg ggcaaacacg tctatttctc
     4621 ccaggtgctg ccggacgcca gctacaagaa gctatcggat tggattctga taaccaaata
     4681 aaagatggga agacgcgggg acgcggagac acggaaagaa aagaaattcc gaaccccaaa
     4741 atccaaattc caatcaggaa tataaatatt ccaattcatc aattttgatt ttggctatta
     4801 tttggaattt ggtgcttggg atttgggatt tcatccgacg atccttttgg aagggcagag
     4861 cgaccgaagg ggctaagaat aagtagaagt ggaaagcagg aagggctatg gcgattctga
     4921 cggttgaaga cttatcgatc agcttcgggg gattgaaagc cttgaacgga gtgaactttt
     4981 cggtggagaa gggcgagatc tactccatca tcggccccaa cggagcggga aagaccacgg
     5041 ttttcaactg catcagcggg atctacaaac ccacgggggg aaagatcctc ttcaaagacc
     5101 aggatatcac ccccttcaag ccccaccagg tggcccaggt gggaattgcc cgcactttcc
     5161 agaacatcga actctttgcc cacatgacca ccatggacaa cctgatgctg gggcggcaca
     5221 ccaagttgaa gaccggcctc tgggcagggg ccaccttttt gaccccgaat tccagggccg
     5281 cccgggagga aattcagaac cgggagaagg tggaggacat catcgacttc ctagaactcc
     5341 agtcctcccg gaaccagatg gtgatgaacc tcccttacgg aaagcagaag ctcgtggaac
     5401 tggcccgcgc cctggccctg gagccggagg tcctcctcct ggacgagcct tcggcgggaa
     5461 tgaacttgga ggagaaagaa gacctgatct tttccatccg ggatattcgg gacgattttg
     5521 ggattaccat tcttctggta gaacatgaca tgaacctggt catggatatc tccgaccgga
     5581 ttctggccct gaactatggc atggtcctgg ctgagggggt tccccagatg attcagacgc
     5641 atccggaagt attaaaggcc tatttaggag aggggtaaac ccgtgggaag catcctccag
     5701 gtgaaaaaca tcgagacttt atattacgac atcattaagg cccttcgggg gatctccctg
     5761 gatattcagg aggggaccat atccgtggtt ctgggaggga acggggcggg gaagaccacc
     5821 accctgaaga ccatcatggg gcttctcgag gaccagcccg acaaagggac catcgagttc
     5881 atgggccggc gcatcgacgg tatggacgcc gaggacgttg tgaatctggg gatcgccatg
     5941 gtacctgaag ggagggaagt cttcccggaa ctgacggtat tggaaaacct caagatggga
     6001 gcctacatcc gtaaggacaa aaaaggggtc caggaagatc aggaccgggt aattcggtac
     6061 ttccccatcc tcaaagagag ggcctcccag caggccgggt acctgagcgg aggcgagcag
     6121 caaatgttgg ccatggggag ggcgcttatg acccgaccca agctcctcat gctcgatgaa
     6181 ccttccctgg gattgtcccc cattctggtg agggagatct ttgagatcat caaaaccatc
     6241 aataaggaag ggacaaccat cctcctggtc gaacagaacg ccaagatggc cctttccgtg
     6301 gctcatttcg gatatgttct ggagacgggt cgaattgtcc tggccaacac cccccagaat
     6361 ctcatggaag acgaggacgt caaggaattt tacctgggaa tcaaatcgga ggtttcggga
     6421 aagggctacc agcgctggcg gcggaaaaaa agatggagat agatcgggtg aattcctctc
     6481 atccatcccc gtacccacca ctcccataat cgggcagata acttttttag aggaggtagg
     6541 acaatgaagc aggagagtac cttttcacgg aggatgcttt ttcaaattgc cggttccacc
     6601 ctggccgccg ccctgttaac cccttccgca ccgctgtggg cccagcaacg gaagctctcc
     6661 atcgccaccg gggggacggg aggcgt
//
LOCUS       DASPRR010000504         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_608512, whole genome shotgun sequence.
ACCESSION   DASPRR010000504 DASPRR010000000
VERSION     DASPRR010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608512"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VEL68_18545"
     CDS             complement(<1..565)
                     /locus_tag="VEL68_18545"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXZ38031.1"
                     /translation="MDTAHFLDPIFKPQSIAIIGASTQVEKWGHRMVARPFNTGFRGN
                     LYPVNPGAKEISGVECFPSISDLPPNIDLAVVTLPAHQVPEALRQCIRQGIRGAVIIS
                     AGFAEAGPDGKKLQDELLSIAREGRIPLVGPNCMGLWSEPVSLNLAFEEAPMRGSLTF
                     ISQSGTLGNYLMLLAKGKGYGFRGFVSG"
     gene            complement(707..1765)
                     /locus_tag="VEL68_18550"
     CDS             complement(707..1765)
                     /locus_tag="VEL68_18550"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXZ38032.1"
                     /translation="MAYQIAVIPGDGTGPEVVNEGLKVLKAVMQKENFKMDFTSYDLG
                     RDRYLKTGEILPDSVLAEFRKFHAIFLGAIGHPDVKPGLLEQGIILKTRFALDQYINL
                     RPVKLYPGVDSPLKDKGPEHIDFVIVRENTEGLYTGAGGVLKRGTADEVAVQESINTR
                     KGVERCLRYAFQFCMKRNRARKLTMAAKTNVLNYASDLWMRVFQELAKEFPQVKTDYT
                     HIDALCMWMVKNPEWYDVVVTDNLFGDIVTDLGAMIQGGLGIAAGGNINPDPGGVSMF
                     EPMGGSAPKYTGKNVINPMAAIAACMMMLDHLGEKKAAERVERAMMQVLAKHLKGMAA
                     GEMGHTTSEVGDLVVKYL"
     gene            complement(1993..2490)
                     /locus_tag="VEL68_18555"
     CDS             complement(1993..2490)
                     /locus_tag="VEL68_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXZ38033.1"
                     /translation="MIFKGKAWKFGANLDTDAIVAARYLNTWDPKELASHLMEDVDPK
                     FREKVQPGDIIVAEDNFGCGSSREHAPMAIKYGGISCVVAKNFARIFYRNSFNMGLPI
                     FESPEAVDGIHPGDELEVDSQTGTIKNITTGQTYHAQPIPPFMQQLIEAGGLMNYVKN
                     LKMKG"
     gene            complement(2569..3843)
                     /gene="leuC"
                     /locus_tag="VEL68_18560"
     CDS             complement(2569..3843)
                     /gene="leuC"
                     /locus_tag="VEL68_18560"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXZ38034.1"
                     /translation="MIRPMTITEQILAEHAGQHEVEPGQLINAKVDLVLANDITAPIA
                     IEEMRKIGATQVFDPAKIVLIPDHFVPNKDIKSAQQCQLMREFARELRLPNYFEVGEA
                     GIEHAFLPEQGLVLPGDLVVGADSHTCTYGGLGAFATGVGSTDIAAAMVTGEAWFRVP
                     ETVKIIYHGKRRPWVDGKDLILYTIGQIGVDGARYKAMEIIGEAIANLPMDSRLTMCN
                     MAIEAGGKNGIIPPDRITKEFVEKRAKRPYKFFHSDSEAKYSRTLDIDVAKIEPQVAF
                     PHLPSNVRPISAVGDVPIDQAVIGSCTNGRLDDLRIAAKVLKGRKRAPFVRLIIIPAT
                     QEIYRTAMKEGLFDIFMDANAVISTPTCGPCLGGYMGILAKGERAVSTTNRNFVGRMG
                     HPQSEVYLANPAVAAASAVLGRLGGPEELGIV"
     gene            complement(3840..>4535)
                     /locus_tag="VEL68_18565"
     CDS             complement(3840..>4535)
                     /locus_tag="VEL68_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HXZ38035.1"
                     /translation="IQVPPNKAIVGANAFAHESGIHQDGVLKEKITYEIMKPETVGIS
                     QSTLILGKHSGRHAFRDWVQSLGYELSEAHLNQAFQRFKEVADKKKQVFDEDLEAIIA
                     DEILRIPDFYRLVNLNVVSGSTCVPTATVQIEIGGKLQQDAAFGDGPVDAAYKTISKI
                     TRTKSHLVKFSVNAITSGTDAQGEVMVILAEGGIRVTGQGAHTDIIMAAAKAYINALN
                     KLEYQKRLKGVSS"
BASE COUNT          910 a         1307 c         1266 g         1052 t
ORIGIN      
        1 caccactgac gaaccccctg aacccgtatc ctttcccctt ggccagaagc atgaggtaat
       61 ttcccagggt gccgctctgg gagatgaaag tgagggaacc gcgcatgggg gcctcttcga
      121 aggccaaatt caagctcacc ggctcgctcc aaaggcccat gcaattcggc cccaccaggg
      181 ggatccggcc ttcccgggcg atgctcaaca attcgtcctg aagcttcttt ccatccggac
      241 cggcttcggc aaacccggcg gaaatgatca ctgccccccg gattccctgc cggatacact
      301 ggcgcaaagc ttcagggacc tggtgggcgg ggagggtaac caccgccagg tcgatattcg
      361 gtggaaggtc ggaaatggag gggaaacact ccactccact gatttctttt gccccgggat
      421 tgacgggata caggtttccc cgaaagccgg tgttgaaagg ccgggccacc atgcgatggc
      481 cccacttttc cacctgggtg gaagctccaa tgatggcgat ggattggggt ttgaagatag
      541 ggtctaaaaa atgagccgta tccataaatc tgtatagaca atgaagggca cgcggcagcg
      601 tgcccccact cctagtttaa accactgata gccgggaacc gccaattgac aatgggcaac
      661 cggcacccag gaacttattt tttaactcga aacctggcac ccgaaactat aagtatttta
      721 ctactaggtc tcccacttcg ctggtagtgt gccccatttc tcccgcagcc atacccttga
      781 ggtgtttggc caaaacctgc atcatggccc tctccacccg ctcggccgcc ttcttttctc
      841 ccaagtggtc gagcatcatc atgcaggcgg caatggcggc catgggattg atgacgttct
      901 tgcccgtgta tttaggggcc gagccgccca tgggctcaaa catggagacc ccccccgggt
      961 ccgggttgat gttcccgcct gcggcgatgc ccagccctcc ctggatcatc gctcccaggt
     1021 cggtgacgat atcgccgaag aggttgtcgg tcaccaccac gtcgtaccat tccgggttct
     1081 tgaccatcca catgcacagg gcatcaatat gggtgtaatc ggtcttcacc tgggggaatt
     1141 ctttggccaa ttcttggaag accctcatcc agaggtcgga ggcatagttc aggacgttgg
     1201 tcttggccgc catggtgagc ttgcgggccc ggttgcgttt catgcagaac tggaatgcgt
     1261 accggaggca cctttccacc cctttacggg tattgatcga ttcctggacc gccacttcat
     1321 cggcagtccc ccttttcagg actcctccgg cgccggtgta gagcccttcg gtgttctccc
     1381 ggacaattac gaagtcaatg tgctccgggc ccttgtcctt cagcggagag tccacgccgg
     1441 ggtagagctt gaccggtcgg aggttgatgt actgatcgag agcaaaacgg gtcttcagga
     1501 tgatcccctg ttcaagaagt ccgggtttca catcgggatg gccgatagcc ccgaggaaaa
     1561 tggcatgaaa ttttcgaaat tcggccagga ccgaatcggg cagaatctct ccggtcttga
     1621 gatatcggtc ccttcccagg tcgtaggagg taaagtccat cttgaaattc tctttttgca
     1681 tgaccgcctt taagactttg aggccttcgt tgacaacttc cgggccggtg ccgtcccccg
     1741 ggatgaccgc gatctggtaa gccataaacc ctccattcct aatttccgat tgcagattgc
     1801 agattttcaa atcctttgaa gctgatgatc ctgcaaaaag tctgtttgat cgtcaccccg
     1861 gcgaaagccg gggtccagaa aatcttaaaa tacctggatt ccggctttcg ccggaatgac
     1921 gggaaagaat atttcttgac tttccacaag accctcaagc ctgaaacctg cgatctgcta
     1981 tctggaatgg ttttacccct tcatcttgag gtttttgacg taattcatca gcccccccgc
     2041 ctcgatcagc tgctgcataa agggcgggat gggttgggca tggtaggttt gcccggtggt
     2101 tatgtttttg atggttcccg tttgactgtc cacttccaat tcgtcgcccg gatggattcc
     2161 gtccaccgct tccgggctct cgaagatcgg caggcccatg ttgaaggaat tccggtagaa
     2221 gatccgagca aaattcttgg ccaccacgca agagatgccg ccgtatttga tggccatggg
     2281 agcgtgctcc cgggaagagc cgcagccaaa gttgtcttcg gcaacgatga tatcgcccgg
     2341 ctggaccttt tcccggaatt tagggtccac gtcctccatg agatgagagg ccagctcttt
     2401 cgggtcccag gtgttcagat accgggcggc gacgatggcg tcggtgtcca ggttggcccc
     2461 aaatttccag gcctttcctt taaagatcat aaaacctcct taaaaacagt tcggagttcg
     2521 aagttcggag tgaaaaaaat atatttcgca tttcgcaacg cgcattggtt atacgattcc
     2581 cagctcttcc gggcccccca gccttcccag gacagcggag gcagcggcca cggcaggatt
     2641 ggcgaggtag acctcgcttt gcggatggcc catccggccc acgaagttcc ggttcgtggt
     2701 ggatacggcc ctttccccct tggccaggat tcccatgtac ccgcccaggc agggaccgca
     2761 ggtgggagtg ctgatgaccg cgttggcatc catgaagatg tcaaataaac cctccttcat
     2821 ggctgtccgg tagatttcct gggtggctgg gatgatgatc aggcggacaa agggagcccg
     2881 cttccttccc tttaggacct tggcagcaat gcgcaggtca tccagacggc cgttggtaca
     2941 ggaaccgata accgcttggt caatgggaac gtctccgacc gcactgatgg gtctgacgtt
     3001 cgaaggcaaa tggggaaagg ccacctgggg ctcgatcttg gccacatcga tgtccagagt
     3061 ccggctgtat ttcgcttcgc tatcgctgtg gaagaattta tagggacgtt tcgccctctt
     3121 ctccacaaac tccttggtga tcctgtccgg ggggatgatt ccgtttttcc cgccggcctc
     3181 gatggccatg ttgcacatgg tgaggcgact gtccatgggg aggttggcga tggcctctcc
     3241 gataatttcc atggccttgt accgggctcc atccacccca atctgaccga tggtgtaaag
     3301 gatcaggtct ttcccgtcta cccagggccg ccgtttccca tggtagatga tcttgaccgt
     3361 ctcgggcaca cggaaccagg cttctccagt gaccatggcg gcagcgatat ccgtggaccc
     3421 caccccggtg gcaaaagccc ccaaaccccc ataggtgcag gtatggctgt cggccccaac
     3481 gaccaggtca ccgggaagga ccaacccctg ttcgggtaga aatgcatgtt cgatccctgc
     3541 ctcccccacc tcgaaataat tgggcagccg cagctccctg gcgaattccc gcataagctg
     3601 gcactgctgg gcggacttga tgtccttgtt gggaacaaag tggtccggga tcaggacgat
     3661 cttggcgggg tcaaaaactt gggtggcgcc aatcttgcgc atctcctcga tggcaatcgg
     3721 ggcggtaatg tcgttcgcga gaaccaagtc caccttggca ttgatcagtt ggcccggctc
     3781 cacttcgtgc tgcccggcat gttcggccag aatctgttcg gtaatggtca tagggcgtat
     3841 catgaggaaa ctcctttaag gcgtttctgg tactccagtt tgttcaaggc attgatgtaa
     3901 gctttggctg cggccataat gatgtcggtg tgggccccct ggcccgtgac ccggattccg
     3961 ccctcggcca ggatcaccat gacttccccc tgggcgtcgg tgccgctggt aatggcattg
     4021 accgagaact tgaccagatg gctcttggtc cgggtgatct tggagatggt cttgtaggcg
     4081 gcgtccaccg ggccatcgcc gaaagccgca tcctgctgaa gcttcccgcc gatctcgatc
     4141 tgaaccgtgg ccgtgggaac gcatgtggag ccgctcacca cattcagatt gaccagcctg
     4201 taaaaatcgg ggatccgcag gatctcgtcg gcgatgatgg cctccaggtc ctcatcgaaa
     4261 acctgtttct ttttatcggc cacctctttg aaccgctgga aggcctggtt gagatgggcc
     4321 tcgctgagct cgtatcccag gctctgcacc cagtcccgga aggcgtgccg gccggagtgc
     4381 ttgcccaaaa tcagagtgga ctgggagatc cccacggtct cgggcttcat gatctcgtag
     4441 gtgatctttt ctttcaatac gccgtcctgg tggatgcccg attcatgggc gaaggcgttg
     4501 gccccgacaa tggccttgtt gggcggcacc tgaat
//
LOCUS       DASPRR010000505         5570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_932961, whole genome shotgun sequence.
ACCESSION   DASPRR010000505 DASPRR010000000
VERSION     DASPRR010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932961"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(446..652)
                     /locus_tag="VEL68_18570"
     CDS             complement(446..652)
                     /locus_tag="VEL68_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HXZ38036.1"
                     /translation="MNVDRLVLTFAGIFILGSLILSQIHSPYWLLFTAFVGLNLFQSS
                     FTGFCPLAKILNAMGVKAGVVFKV"
     gene            797..883
                     /locus_tag="VEL68_18575"
     tRNA            797..883
                     /locus_tag="VEL68_18575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:831..833,aa:Leu,seq:cag)
     gene            1447..2037
                     /locus_tag="VEL68_18580"
     CDS             1447..2037
                     /locus_tag="VEL68_18580"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ38037.1"
                     /translation="MSLVFLFAKGFFSLTFPPFFRYVLPQIEFVSFHFLRLDSTLNAG
                     IVMWLMDRISSAVLFAFVIFYTAAGWGLETWAKKAPGPGFMPRLLALALGALAILIWV
                     QAKPSLESRQEVESYSKREPFIIFLLVLAYVFVMPQLGFPLSNFLLVIALRRLVEPGS
                     WRADLAGAIGTVAIIHLVFVQILALQFPVFPVWWGE"
     gene            2042..3559
                     /locus_tag="VEL68_18585"
     CDS             2042..3559
                     /locus_tag="VEL68_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ38038.1"
                     /translation="MDSIHGLLYGLSRAVEPQILILAFLGCFWGTMVGVLPGIGPVAG
                     ITILMPFTFGIDLTSAMVMMAGIYYGAMYGGSTTSILVAVPGETSSIVTIFDGHQMAK
                     QGRAGAALALSAIGSFVAGTVGILGLTFLAPPLAELAIGFGPPEYFALALVGLSMSSF
                     LSNASLAKGLAMAVVGLITGTVGLDIISGAPRFTFNSMELAGGLEIVSILIGLYGISE
                     VMEMMASKVRPQIQHEKLWNLHRLLPNREEWRLSIWPIIRGSFMGFFVGALPGAGGTV
                     PSFISYAMEKRISKNPERFGKGAPEGVAGPESANNSGAVGAMVVLLTLGLPYNVVTAV
                     MLSAMIAHGIFPSPLLMARSPDFFWTVVASMYIGNFMLLILNLPLIGAWASLLRLPYR
                     WMTVFIVLFCIIGVYSIEYRSFDLLLMALFGGIGYLMKRVDLPAAPLALAMVLGPIME
                     KSLIQSLIMSDGSPFIFVTRPFAAGSMALFFLLMFLPLIQKKLGRRIAPVGEVEV"
     gene            3658..4635
                     /locus_tag="VEL68_18590"
     CDS             3658..4635
                     /locus_tag="VEL68_18590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ38039.1"
                     /translation="MKENQLRVLLGILALVFIVAFGSQSQAASYPARDVTMIITWGAG
                     GTSDLSCRQLADPLGKILGKSIIPLNVVGGSGAIGMNQGAHARPDGYTLTFCTSGPTV
                     IQPWMQKVTYDPVKDFIPVAQVFGEYLVVVAKADAPYKNLKEMVDYFKKAGKAPKFAT
                     PGAGSNNHLSMVLMSKATGLAMTHVPFSSSAEGVNAVIGGNVDFASTEPPSAVGPYKE
                     GRANILAILSAQRLDLIKDIPTVKEQGIDLVFGGWNGILAPKGTPKEIVDKLAEAAKK
                     ALENETLIKNSATVGRPIEFLSGPEFGERIKKELARNGEIIKAEGLAPK"
     gene            5048..5560
                     /locus_tag="VEL68_18595"
     CDS             5048..5560
                     /locus_tag="VEL68_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38040.1"
                     /translation="MVNRAPVLTLWAAVVAEVLGFEHDEALTLGRAVAGLNAYLKGVS
                     LGLFQPTPTDVKEKRRKMRKDETVTIDLLHRAVPAKHTDEGLRALSGESPIRPESVQK
                     YLEGKFGDALEDVSNAMWELAKSLPPSQLAEKAYGLYEKFRPEIPPGKKGWGASGKLD
                     LDLIRKMASA"
BASE COUNT         1181 a         1514 c         1552 g         1323 t
ORIGIN      
        1 tgccttttat tataagcgat ccagggaaat cgaggaatgc cgattacgtg ttgaacctcc
       61 ccgcccacag ggcggaaaga ttcactatcc caggaaagga aacaagtcaa tcgttttgct
      121 aatgtcgcct cccctaggtt cagtgaataa atttaccggg aggggggcgg aaaaatgatg
      181 cgtgccacca aaatcggcgg ggaaatggcc tttctccgag cggtgacctg gtatccgcaa
      241 acgacgcttc cggcctccgg gaaattaggc taagagcgca aaaatagaca atttccgaaa
      301 cctttagtaa gaatcaactt tttcaaagag gaacgagctt cttattttgg caagcgcagc
      361 ggttcttcgc cccgctctct gctcgggagc agaattaggc agcttatttc tgagacgcga
      421 cactaggcta aggaggttgg aaggatcaaa ccttaaagac gacgccagct tttactccca
      481 tcgcgtttag gattttggcc agggggcaga agccagtaaa cgaagactgg aacaggttga
      541 ggcctacaaa agctgtgaaa agcagccagt aaggagaatg aatctgggac aaaatcaaac
      601 ttccaaggat gaatatccct gcgaatgtaa gaaccaatcg atccacgttc atttccagca
      661 cctcccttta tttattaaga tgtatttcat ctccagcagg attcttccat tttttcttcc
      721 ttttgggatg gcgggaaaaa atccttgaca atgatttgtt ttaattgtag gtttttacta
      781 tccaattgaa ccatgtgccg aagtggcgga attggtagac gcgctaggtt cagggtctag
      841 tgtgggttgc cacgtagggg ttcgagtccc ctcttcggca ccaaggttta gaaaaggccg
      901 caattctaat gggttgcggc cttttcattt ttgcagtgtg cgcctggggt gcaaaagagc
      961 ccagattttg cgggctctct gaatgggtct ttcctcagca acttcttact ctgtatcaat
     1021 tgacaggaat aggttcattt ccttgaacac ccttggattt atgtgtgaca aaaggctgta
     1081 gaatcggctg atattttttg cagaaattgg tattctgtac ccggaatacc cgcaaaaggc
     1141 agataaagac tccctcctgt agtcaccgcg tgacagattt tgacagatgg tgtagatttc
     1201 tatccgtaat ggaggtttgg atatactttt aatgtgagaa attattacga agtgcggaga
     1261 tttgcacgct gtacgggaca aattttgttt tttctaatac aaacgaaatt attattttac
     1321 cccccaccct ccccctcccc ttgactccgg tctatacacc cctcaagggg ggggtaaggg
     1381 tgggggtgat gtcatttttt ttaatgcgtt tgtattagta tgatcgctcg attagatgtt
     1441 attagcatga gtctggtttt cctttttgcc aagggctttt tttcattgac atttcccccc
     1501 tttttccgat atgttctccc ccaaattgag tttgtatcat ttcatttcct caggctggat
     1561 tccactttaa atgctgggat agtcatgtgg ctgatggacc gtatttccag cgccgtcctc
     1621 tttgctttcg tcattttcta taccgccgcc ggttggggct tggagacatg ggccaagaaa
     1681 gcgcccggcc cgggctttat gccccgcctc cttgctctcg ccctcggcgc gctcgccata
     1741 ttaatatggg tgcaggctaa accttccttg gagagccggc aggaggtcga atcctactcg
     1801 aaacgcgagc cgtttattat ctttttgctc gtcctcgctt acgttttcgt gatgccgcaa
     1861 ttgggattcc ctctctctaa tttcttattg gtcatcgcct tgagacgcct ggtcgaacca
     1921 ggctcgtggc gggctgatct ggcaggagcg ataggaaccg tcgcgatcat tcacctggtc
     1981 ttcgtccaga tactggcact tcaattccca gtgtttccgg tgtggtgggg agaataatcg
     2041 aatggactca attcatggcc tgctttatgg actgagccgg gccgtggagc cgcagatcct
     2101 cattttggct tttctcggct gcttctgggg aaccatggtg ggggtcctgc ccggcatcgg
     2161 tccggtggcc gggataacca tcctgatgcc cttcaccttc ggcatagacc tcacctccgc
     2221 tatggtgatg atggcgggta tctattacgg ggccatgtac ggggggtcca ccacgtccat
     2281 tctcgtcgcc gttcccgggg aaacgtccag catcgtgacc atcttcgacg gtcaccagat
     2341 ggccaagcag ggacgggcag gggcggcgct ggccctgtcg gccatcggtt ccttcgtcgc
     2401 cggaaccgtc ggcatcctcg gcctgacctt cctggcccca cccctcgccg agctggccat
     2461 cggcttcggc ccccccgagt atttcgccct ggccctggtg gggctctcca tgtcttcttt
     2521 cctctccaat gcctccctgg ccaagggact ggcgatggcg gtcgtcgggc tgatcaccgg
     2581 caccgtgggg ctggatatta tctccggtgc gccgcgcttt accttcaact ccatggaact
     2641 ggccggcgga ctcgagatcg tctccatcct gatcggcctc tacggcatct ccgaagttat
     2701 ggagatgatg gcgagcaagg tccggcccca gatccagcac gagaagctgt ggaacctcca
     2761 ccgtctcctc cccaaccggg aggagtggag gctatcgatc tggccgatca tccgtggaag
     2821 cttcatgggc ttcttcgtcg gtgcgctccc cggcgcagga ggcactgtcc cctccttcat
     2881 ctcctacgcc atggagaagc ggatctccaa gaaccccgag aggttcggaa agggggcccc
     2941 cgagggggtg gcgggacccg agtcggcgaa caactcaggg gccgtgggcg ccatggtcgt
     3001 cttgctcacc ctggggctgc cctacaacgt cgtgaccgcg gtcatgctaa gcgccatgat
     3061 tgcccatggc atcttcccga gcccgctgct aatggcccgg agtccggatt ttttctggac
     3121 ggtggtggcc agcatgtaca tcggaaactt catgctcctc atcctgaacc tgccccttat
     3181 cggggcatgg gcttcgctgt tgcggctccc ctatcggtgg atgaccgtgt tcatcgtact
     3241 cttctgcatc atcggcgtct attccatcga ataccgttcc ttcgaccttc tgctgatggc
     3301 cctcttcggg ggcatcggct acctcatgaa gcgggtggac ctgcccgctg ctccgctggc
     3361 tctggccatg gtcctcggtc ccatcatgga gaaatcgcta attcagtccc tcatcatgtc
     3421 cgacggcagt cccttcatct tcgtgacccg tccctttgcc gcaggcagca tggccctctt
     3481 tttcttgctc atgttcttgc cgctcatcca gaagaagctg gggaggagaa tcgcccccgt
     3541 gggagaggtg gaagtgtaga ccgcgccttg ggggagtcct tttccccacc tggccatccg
     3601 acacttgggc gcgcttacct gggtcttagg gaaccaactt tggaaaggag agagagtatg
     3661 aaagaaaatc aacttcgagt tctgctggga attctggctc tggtgttcat cgtcgccttc
     3721 gggagccaat ctcaagccgc ctcctacccc gcccgggatg tcaccatgat catcacctgg
     3781 ggagcggggg ggaccagtga cctgtcctgc cgtcagctgg ccgatccctt ggggaagatc
     3841 ctcggcaagt ccatcatccc gttaaacgta gtgggggggt ccggggccat cggcatgaat
     3901 cagggcgccc atgccaggcc tgacggctac accctcacct tctgtacctc cggtcccacg
     3961 gtaattcaac cctggatgca gaaggtgacc tacgacccag tcaaggattt cattcccgtg
     4021 gcgcaagtct tcggcgaata cttggtcgtg gtggctaaag ccgacgcgcc ttacaagaat
     4081 ttgaaggaga tggtggatta cttcaagaaa gccggcaagg cccccaagtt cgctaccccc
     4141 ggcgccggca gcaataacca tctatccatg gtcctgatga gcaaggcgac cggtctggcg
     4201 atgacccacg tgcctttcag cagctcggcc gagggtgtga acgcggtgat cggcgggaat
     4261 gtggattttg ccagcaccga gccccccagc gccgtcggcc cctataagga aggccgcgcc
     4321 aacattttgg cgatcctgag cgcccagcgg ctcgacttga tcaaggacat cccgacggtt
     4381 aaggaacagg gcatcgacct ggttttcggc ggctggaatg gcatcctggc gcccaaagga
     4441 acccccaagg agatcgtcga caagctagcc gaggcggcaa aaaaggcgct cgagaatgag
     4501 accctgatca agaactcggc cacggtagga cgaccgatcg agttcctctc cgggccagaa
     4561 ttcggcgagc ggatcaagaa ggaactggcc cgcaatggag agatcatcaa ggcagagggg
     4621 ctggccccta agtagcgatg gatgattcag gagcctgagt ccgtagttgg tttccttact
     4681 cttccactta tttctgggct caggggtggg gccgaagact ggggtaccct ttctggcgga
     4741 aaggattggg gctaaaatgt cgaattgtct tatacagaaa gaagccgtct gcctttgcag
     4801 ggacatacag actgcataat tattgttggg ctcaagagat gacccgaaac cagacgatag
     4861 tgcctgagga gatatggggt caggtagatg cccggcgtag gcctactgtc tttgccctcc
     4921 ggatgacaaa cggcacgatc ctcagggatg tcgccatcaa aaaatcggaa cgggagatcg
     4981 aacacggcat ctgatccctc gatgaaatga atccatgcac gagaaaagga taaaaagaag
     5041 ttgggtaatg gtgaatcggg cgccggtgtt gacgctttgg gcagcagtgg tggctgaagt
     5101 cctggggttc gagcatgatg aagctctgac cctagggcgg gcggtggctg ggcttaacgc
     5161 ttacttaaaa ggggtttccc tgggactctt ccagccgact cccacggacg tcaaggaaaa
     5221 gcgccggaag atgcgcaaag atgaaacggt gaccattgac ctgctacacc gtgctgtacc
     5281 ggccaaacat actgacgaag ggttgcgagc actcagcgga gaaagcccca tccgtccaga
     5341 gagcgtacag aagtacctgg agggcaagtt cggtgacgct ctcgaagacg tttctaatgc
     5401 tatgtgggag ctggcaaagt ctttaccccc ctcccagctg gcggaaaagg cctacggcct
     5461 gtacgagaaa ttcaggcccg agattcctcc cggcaagaaa ggatgggggg cctccggcaa
     5521 acttgacctg gacctcattc gcaagatggc ttcggcctga ccctgaggat
//
LOCUS       DASPRR010000506        14480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_438649, whole genome shotgun sequence.
ACCESSION   DASPRR010000506 DASPRR010000000
VERSION     DASPRR010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438649"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1079
                     /locus_tag="VEL68_18600"
     CDS             198..1079
                     /locus_tag="VEL68_18600"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38041.1"
                     /translation="MHIDLPALIVSAILLAGLYATMSYGLALIYGVMKIVNLAHAGVM
                     MWGAYLAFIFIVNLGMNPFLAPVLIVPTFFFLGILLQRYIVSRIMSAPPISSLLLLFG
                     VWLIMQNISYLIFSGDTRAITTPYTLSTFLVGGISISVNRLVVFGVGILALVSLRQFL
                     GHTYLGKAIRATAQDGEASKLVGINTQWVMEMAFGIGIALASLAGSLMALIFAFDPDF
                     GRSHLLKGFCIVVLGGLESMVGVALGSLVLAIAEALSVLWIMPAMQDFVSFAILVIVL
                     VVMPGGMLGLLQKFLKT"
     gene            1108..2169
                     /locus_tag="VEL68_18605"
     CDS             1108..2169
                     /locus_tag="VEL68_18605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38042.1"
                     /translation="MSRAWKMGMSLLGLAFLVWIPFSGIGGGYLQTLGFLTFLYVTLS
                     TAWNIMGGYTGQISFGHATFYGLGAYTTSILTLRGLNPFLTIPLAGIVASLYGLLWGY
                     PCLRLRGQYFAIATIGVGEATRLLMLNMDDLIKNNDWLKGFLHDGVLTGGATGLMLPT
                     PPDIRSYALGFYYGAMGIMFAALLVSWKVKTSKFGLGLLAINMDIDAAETLGVRTAYY
                     KNLALMLSALMVGICGSIYSQYVFYIDPQTVFGFPMSIAMVLMPTIGGIGTLLGPVLG
                     AVVYVVVQDQILTANLEFGDFKLSLSSLHLLIYGGLLVVIVLFEPKGILGLQKRLARK
                     WAKRKEDSRERDDNAARVR"
     gene            2182..2922
                     /locus_tag="VEL68_18610"
     CDS             2182..2922
                     /locus_tag="VEL68_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38043.1"
                     /translation="MAVIIEGKNVSKYFGGLIAVNDVSFQVHQGEILGLIGPNGAGKT
                     TLFRCLTGVYPVTNGRISFRGEEITKLSPHAICVRGITNTHQVVKPFPDMTVLDNVRV
                     GAFFGRGWVRGEVKDAAREASRVLEFTGLLPKANFQARNLTLPDRKRLEVARALATRP
                     EVLLLDEVVAGLNPTETDMMIRLIRQMRDNGITILMVEHVMRAVLGVSDRVMVLNFGQ
                     KIAEGTPQEVSANPQVIEAYLGKGFGAT"
     gene            2992..3696
                     /locus_tag="VEL68_18615"
     CDS             2992..3696
                     /locus_tag="VEL68_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38044.1"
                     /translation="MLRVERINVFYDDAQALWDVSFAVNRGEIVTLVGSNGAGKSTTL
                     KALSGLVPPISGEILFDGSRIDRLPAHRIVEMGIAHIPEGRRLWPGLTVRENLELGAY
                     SRVARAARGDTMAGVFQLFPRLDERKNQLAGTLSGGEQQMLAIGRGLLSKPQLLVLDE
                     PSLGLAPLLVNDIFGTIQKINRQGVTVLLVEQNVNYALVISHRGYVLETGRIVLHGPG
                     KELLANEHVKTAYLGL"
     gene            complement(3983..4267)
                     /locus_tag="VEL68_18620"
     CDS             complement(3983..4267)
                     /locus_tag="VEL68_18620"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXZ38045.1"
                     /translation="MATFLMFGKYSAEAVKKISAKRTKDAANLVKKYGGRVEAMYALL
                     GEKDIVFILNFPGVGQAMKASVALSKMTGIGFSTYPAVSVEEFDRLMARK"
     gene            complement(4376..5164)
                     /locus_tag="VEL68_18625"
     CDS             complement(4376..5164)
                     /locus_tag="VEL68_18625"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HXZ38046.1"
                     /translation="MGYVKTSDELAKVYRETYDFYDAEMLTVVWETKPEVVKRLLPPP
                     LKPGKRPIALAFVANYPRTNFGVSYLESALFLRAEFSGEEGNYCLAMPVTNDMALVLG
                     REVFGYPKKMANIQFRRQGKEIEGLTERHGQIFFEVKARMTGQLNFPEAQNVLMETFR
                     FGASLVVITFNFKYFMAPEGDGFDYPPRLMREEVEFRPRAIEIGEVELKLHRSEFDPW
                     AEVEPVRVLGAFYTIGHNSMRKGKIVAETDPKGFIPFSFMKTDF"
     gene            complement(5328..6590)
                     /locus_tag="VEL68_18630"
     CDS             complement(5328..6590)
                     /locus_tag="VEL68_18630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ38047.1"
                     /translation="MGIRELNVDLTEEHIALRDAARKFLEGVWRPASIALDKLSDPQE
                     VIAHNSILWDVFRRTQELGYHKMPFPKAVGGLELDILSMSLISEEMGWAAPDLAVSLA
                     VSVTPFAYAQLSPESEVRDLTRKFCEDTDARMIGCWAITEPDHGSDWILLDVEEGKNP
                     KIVPQVRAVPDGDHYLINGQKSSWVSNGSIATHAALWVSLNPSIGMEGGGIAVVPLNL
                     PGVFRGKPLNKLGQRALNQGEIFFDNVRVPKSMMIAQEPMGFKFMSHAQLALANGLMG
                     LCFAGTAQAALEEAIKYARTRVQGGQPICKHQAVKLKLFDMFVSVEAARSLARRVAVY
                     NGGLLKKLQLPAVHYAMAAKVLSTETAFRVASEAIQVYGGYGLCKDYYVEKIFRDTRA
                     AMIEDGTNEVLSLGGADRLMSAKVRGEV"
     gene            complement(6972..7412)
                     /locus_tag="VEL68_18635"
     CDS             complement(6972..7412)
                     /locus_tag="VEL68_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008079132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXZ38048.1"
                     /translation="MRKATFLLFFLFLSNFAFGQGAEIHQAAYHGDIDKVRELLQKGV
                     KPDERDSFGGTALHAAMFQKNMEIVKLLLQHHFDINAQGTQNGYTPLHDAVWANNMEA
                     IKLLLDEGAKTNLKGRDGLTPYVKAKKEGKTEIVKLFESRKIKE"
     gene            complement(7481..8146)
                     /locus_tag="VEL68_18640"
     CDS             complement(7481..8146)
                     /locus_tag="VEL68_18640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ38049.1"
                     /translation="MKVDSDLYAYTWKSSMENNCNSYVIGGDIPIIIDPGHQHLVKNL
                     IKQMEKDGNRFEDIRLIIATHGHPDHFEGTQTFARGGVQVAIHEEEEKFLNEIGSQFY
                     RAMGGEMPELKISFYLKEGELKVGSKTFQILHTPGHSPGSISIYWPERKALFTGDVVF
                     PMGVGRTDFPGGDGMLLRESIERLAKLDAEWLLAGHGEVIKGKKNIQRNFAYIRTNYY
                     DYL"
     gene            complement(8239..8943)
                     /locus_tag="VEL68_18645"
     CDS             complement(8239..8943)
                     /locus_tag="VEL68_18645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXZ38050.1"
                     /translation="MTKGVTLYRISQAYKVPIARLMEANKLSSPSAVKVGQKLFIPGA
                     KAVLPVEPYIPLSPSEKKKLEESLDTGEPPAPLPPLSEYNKATQDSEKPPWWGKELDI
                     IWPIQGKITSPFGPRGKRFHGGIDIRSPSYQEVKAAMDGEVILARNTQTGYGKVVFLR
                     HDQGFLTIYGHLNVIMANEGEAVRQGQSIGGVGSTGRSTGPHLHFELRHNSRPLNPLP
                     LLPPTIEELLEKATKR"
     gene            complement(9168..9875)
                     /locus_tag="VEL68_18650"
     CDS             complement(9168..9875)
                     /locus_tag="VEL68_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38051.1"
                     /translation="MLNVRDLVVRIGRIEALRGASLEVVEGTIVALLGANGAGKSTTL
                     KTISGLLRPLSGEVLFQGEPIHRSTPREVVALGIVQVPEGRKLFPRMSVLENLEVGAY
                     LRSDSRAALQQDLERIFEHFPVLRERRRQEAGSMSGGEQQMLAIGRGLMARPKLILLD
                     EPSLGLAPLMVEEIAKIIGEINHSGVTILLVEQNAEMALSIAHHGFVLETGRITLHGS
                     ASELLSSETIRLAYLGT"
     gene            complement(9859..10665)
                     /locus_tag="VEL68_18655"
     CDS             complement(9859..10665)
                     /locus_tag="VEL68_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38052.1"
                     /translation="MFEEKGKVFNHILEVQGLTRHFGGLAAISDFNLAVRPGEFFGLI
                     GPNGAGKTTVFNLISGVLPPTRGKVHFSGADITGMPSYALPRLGLARTFQITALFQSF
                     TVLENVLLGTYGVFRINPLATLWNTRRAGETERAQRERALDLLRFLGIDRLRDEVARN
                     LPLGHQRILELAVALASSPKMLLLDEPFSGLSAEESREMMARIQQVREEKITIMLVEH
                     NVRIVMELCEKIAVLNFGRKIAEGTFAEVSRHPEVIKAYLGTKRYAAQRS"
     gene            complement(10658..11599)
                     /locus_tag="VEL68_18660"
     CDS             complement(10658..11599)
                     /locus_tag="VEL68_18660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38053.1"
                     /translation="MSLPPFLIVAAVLAGLPILIGDPYWLHVYIMTCLNIILAASLRV
                     LWNVGLLSCGHAAFMGIGAYTSALLAKEIGLSPWLGMLAGGGLSVVVSVVLGYPALRL
                     TGIYFVLVTFAFNEVFFLIATRWREFTGGPSGLLGIPRPEGIPAGKLAYAYFASILLW
                     LSLIILFRFEQSRVGRIWLAIRDTELRSRCVGIQTTHYKLFAFAVSSFFAGVAGAVYA
                     HYIGFISPTDFTIWQSIYTQIYMIVGGVAAFAGPILGSVVLTVLSEFIRAVGPLQSVV
                     YGTMLAVVMLFSPGGLVSLGPYFSGWFKKRKEASARV"
     gene            complement(11601..12467)
                     /locus_tag="VEL68_18665"
     CDS             complement(11601..12467)
                     /locus_tag="VEL68_18665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38054.1"
                     /translation="MDSFPFYMQVFTNGVVLSCYYILVALGLNLIMGIMHIVNFAQGT
                     VYMLSAMLLYILCEELGLDYFSSGALAIAGTAGLGILMERFLFRRLLGQFLPSLVMSL
                     GLLLAIEGAVWLRFGIISKAVSSPFRGLLKFWGASISYERLVVIVGGLLLIVGLFIFI
                     RHTKAGKAIRAVEQDREAAILHGIEITKICRLVFVVSFALSAAAAFLVSPMLVIDPNI
                     GHTPLLKGLVIIVLGGMGSVTGTLAGGFIIGFIESIAGSIFDPTIASIFSFVILVGIL
                     LVRPSGLLPYRM"
     gene            complement(12810..13991)
                     /locus_tag="VEL68_18670"
     CDS             complement(12810..13991)
                     /locus_tag="VEL68_18670"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38055.1"
                     /translation="MGKLFRPSILALSISLVVIPFAFNPGLAKDLVIGINTPLSGAAA
                     PTGLGLLRAVELAAEDINGAGGIKVGKETYTFKLITYDNKYDAKEAVASVNKLIYNDK
                     VKYIVTTGGTCVIAVNPLINENKVLHIGYAYGGKKATNPSTPYTFRSIMEPIQGHSAL
                     LPYIVEKFKVKTIALTSTDDETGLIQAEDAEIVAKKLGLAITDKSFAPRGTADFTPML
                     TKLIAKKPQAIDFGAWAGSDGPLACKQAKELGYTGVMIFSYSQSIPTFEKVAAGFMDG
                     VLFYGIYATDPSPIATKVAKQYEGKYKQKFDPIVWRNSDVGYVLKKAIEMAGSVDSTA
                     VRDAMPKVQIDGVFGRTRIGGKSYYGIDCQFLFPVPLTTYDTKQKKLVELHRGGMPEG
                     Y"
     gene            complement(14052..>14480)
                     /locus_tag="VEL68_18675"
     CDS             complement(14052..>14480)
                     /locus_tag="VEL68_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38056.1"
                     /translation="FDVTLAPGEITEAEKEFRQSYHRMYDNDEWFFQKSTRRRFAQLP
                     PRAKFSQFVYKVSGGPILRVNLAVLEDQVLDVLITGNMMPSRREIPEEIEQALRSAPA
                     MEAEIEKIIRQKWEKEKMIVAGARVEDFITAVSGALRALS"
BASE COUNT         3209 a         4006 c         3929 g         3336 t
ORIGIN      
        1 ccctcgtggt cggaggacgc gtttggttta aacccaatgg gcaaatcgac aacgactacg
       61 tgatgatgca aaacctcccg ggagcaaaag tttccttaat ctatcccccg gaggtggcca
      121 cggcgaaagc catcgtgccc attcccaaga aatagaaaga cccgtccatc ctcttgggag
      181 aattcgagcg agggaaaatg cacatagacc tccccgccct aatagtcagc gcgattctac
      241 tggccgggtt gtatgccacc atgtcttacg gcctggcctt gatctacggc gtcatgaaaa
      301 tcgtaaacct ggcccatgcc ggggtgatga tgtggggcgc ctacctcgct ttcatcttca
      361 tcgtaaatct gggaatgaat cccttcttag cccccgttct gattgttccc accttctttt
      421 tcctgggaat cctactgcag cgttatatcg tcagccggat catgtcggct ccccccatct
      481 cttctctcct ccttctcttc ggagtctggc tcatcatgca gaatatctct tacctcatct
      541 tctccgggga tacgagggcg attaccaccc catacaccct ttccaccttc ctggtcggag
      601 ggatttccat ctccgtcaac cgtctggtgg tatttggcgt ggggatttta gccttggtca
      661 gcctccggca attcctaggg cacacctacc tggggaaagc catccgggct acagcccagg
      721 atggggaggc gagcaaactg gtgggaatca acactcaatg ggtcatggag atggccttcg
      781 ggatcgggat tgctctagcc agcctggccg ggtccctcat ggccctgatt tttgccttcg
      841 atcccgactt cggaaggtcc catctcttga agggattctg tatcgtcgtc ctaggcggcc
      901 tggaaagcat ggttggagtt gctctgggtt cattggtctt ggcgatcgcc gaggctctaa
      961 gcgtactctg gattatgccg gcgatgcaag acttcgtcag tttcgccatc ctggtgatcg
     1021 ttctggtagt gatgcccggc gggatgctgg gcctgctgca aaaatttttg aaaacctgaa
     1081 tttccaagga gccccgggag aggaaaattg tcccgcgcct ggaagatggg gatgagttta
     1141 ttgggtttgg ccttccttgt ctggatccct ttctccggga tcgggggggg ataccttcag
     1201 actctcgggt ttttgacttt cctctacgta actctgagta ccgcctggaa tatcatgggg
     1261 ggctatacag gtcaaatctc cttcggccat gcgacttttt acggtctggg agcttatacc
     1321 acaagcatcc tgactctccg gggcctaaac ccgttcctga ccattccctt ggccggcatc
     1381 gtggcttcct tgtacgggtt gttatggggg tatccctgtt tgcgcctgag gggccaatac
     1441 ttcgccatcg ccaccatcgg cgtgggggag gcgacccggt tgttaatgct caacatggat
     1501 gacctgatca aaaacaacga ctggcttaaa ggattcttgc acgatggcgt cctcaccgga
     1561 ggagccaccg ggctcatgtt gcccacaccc ccggatatcc gttcttatgc cttgggcttt
     1621 tattatggag ccatgggcat tatgtttgcc gccctgctgg tctcctggaa agtgaagacc
     1681 agcaagtttg gcttgggact cctggccatc aacatggata tcgacgccgc ggaaacattg
     1741 ggggtcagga ccgcttacta taagaatcta gccctcatgc tgagcgctct catggtgggc
     1801 atctgcggga gcatctattc ccagtatgta ttctacatcg atccgcagac ggtattcggc
     1861 tttcccatga gcattgccat ggtcctgatg cccactatcg gcggcatagg gactctatta
     1921 ggtccggtat tgggagcggt cgtttacgtt gtggtccagg accagatcct aaccgccaac
     1981 ttggaattcg gggatttcaa actctctctg agcagcttgc acctcctgat ctatggaggt
     2041 ctattggtgg tcatcgtcct ctttgagccc aagggaattc tgggcctgca aaagcgactg
     2101 gccaggaaat gggccaaacg gaaagaagat agccgagaaa gggatgataa tgcagcccgg
     2161 gtcagatgag gtttatcgct aatggcggtg atcatcgaag gaaagaatgt cagtaaatat
     2221 ttcggcggac tgatagcggt gaatgatgtt tctttccagg ttcatcaagg ggaaatcttg
     2281 ggtctcatcg gccccaatgg tgccggcaag acaactcttt ttcgctgtct taccggggtc
     2341 tatcctgtta ccaacgggag gatttccttt cgcggcgagg agatcaccaa attaagccct
     2401 catgccatct gcgtacgggg catcaccaat acgcaccagg tggtgaaacc ttttcccgac
     2461 atgaccgtcc tggacaacgt tcgcgtggga gctttcttcg gccggggctg ggtgcgggga
     2521 gaagtgaaag atgcagctcg agaggcctct cgcgttctgg agttcaccgg gctgctgccc
     2581 aaagcgaatt ttcaggcccg caacctgact ttgccggacc ggaaacgtct ggaggtggcg
     2641 cgagcgctgg cgacccgtcc tgaggtatta ctgctggatg aagtggttgc cgggctcaat
     2701 cccacggaga cggacatgat gattcgcctc atccggcaga tgcgggacaa cggaattacc
     2761 attctgatgg tggaacacgt catgcgggca gtcctgggag tgagcgatcg ggtcatggtc
     2821 ctcaattttg gccaaaaaat agccgagggg accccccagg aggtatcggc caatcctcag
     2881 gtgatcgaag cttacctggg aaaggggttc ggcgcgacct gacggtccgg gagaaggtcc
     2941 gccatgggat cacccgggat cgagtgagat ccttgcgggg aatgaaacag tatgctccga
     3001 gtagaaagaa tcaacgtatt ttacgatgac gctcaggcct tatgggatgt ctctttcgct
     3061 gtaaaccgag gagaaatcgt taccctggtg ggttcgaatg gggccggcaa gagcacgacc
     3121 ctgaaagccc tctccgggct ggtacccccc atttccggcg agattctttt cgatgggagc
     3181 cgcatcgatc gtctgccggc tcaccgcatc gttgagatgg gtatagccca catcccggaa
     3241 gggcggcgct tatggcccgg cctgacggta cgggagaatt tagaactagg agcttactcc
     3301 cgcgtggctc gcgctgctag gggagacaca atggccggag tatttcagct cttcccccgt
     3361 ttggatgagc ggaaaaacca attggcgggg actctttccg gaggagagca gcagatgttg
     3421 gccatcggga gggggctcct ttccaaaccc caattgctcg ttctggatga gccctcttta
     3481 ggcctggccc ccttattggt caacgatatc tttggaacca tccagaagat caaccggcaa
     3541 ggtgtaaccg tcctgctggt tgaacagaat gtgaactatg ctctagtcat ttcccatcgg
     3601 ggatacgttc tcgagacggg ccggattgtg ctccatggac cggggaaaga attactcgcc
     3661 aacgaacacg taaagaccgc ttacctgggc ctctaactct aaccccttac ggctgcctct
     3721 tgcgggcgtt cctcagagga ttcagccttt ccactgcccc tttttttcag ggttgggcaa
     3781 tttcccggct caacttcctg gtcccgaata agaagggaat tgcccgtatc gctgggggca
     3841 atacagcact gggaagaagc ggataagagt atccccagag tgggggttgt ggccttccgt
     3901 ccggcggaat aaggattcat cccccgggaa ccgttcgggc ggaaggcctc tcaggagttt
     3961 ctggccgggc aaagtctcgg cgtcacttcc tggccatcag cctgtcgaat tcttccacgc
     4021 ttactgccgg ataggtggag aagccgattc cggtcatttt actcagggcc accgaagcct
     4081 tcatggcttg tcctaccccc ggaaaattca ggataaaaac aatatccttt tctccgagaa
     4141 gggcatacat cgcctcgacc ctgcccccat atttcttgac caggttggca gcatccttgg
     4201 tgcgcttggc gctgatcttc ttcaccgcct ccgctgaata cttaccgaac atcaaaaacg
     4261 tagccatggt aattcctcct ttcgaggttt tcttttgccc cccttcataa atggggggat
     4321 ttctggattt gcccagattt gaggaataac accttcttga cgaccttccc gtaatttaga
     4381 aatccgtctt cataaaagaa aatgggatga atcccttcgg atcagtttca gccacgattt
     4441 tgcccttccg catggagttg tggccgatag tgtaaaatgc ccccaggact cttaccggct
     4501 ccacttcagc ccagggatca aattcggacc ggtgtaactt cagctccacc tctccaatct
     4561 cgatggcccg tggacggaat tccacctcct cccgcatgag cctcggagga taatcaaatc
     4621 cgtctccttc gggagccata aaatatttga aattaaaggt aatcaccacc aggctggcgc
     4681 cgaagcgaaa cgtctccatg aggacattct gcgcctcagg aaaattgagt tgtccggtca
     4741 tcctggcttt cacttcaaaa aagatttgtc catgccgttc ggtcaaccct tcgatttcct
     4801 tcccttggcg gcggaactga atattcgcca ttttcttggg atacccgaag acttcccgcc
     4861 cgaggaccag ggccatatca ttggtcacgg gcatggccag gcaatagttt ccctcctccc
     4921 cgctgaattc tgcccttaga aaaagggcgc tctccaggta gctgacgccg aagttagttc
     4981 ggggatagtt ggcgacaaag gccagggcaa tgggtctctt tcctggtttg agcggagggg
     5041 gaaggagccg tttgacgact tccggctttg tctcccagac cacagtcagc atctccgcat
     5101 cgtagaaatc ataggtttcc cggtagacct tggcaagttc atcggaagtc ttcacgtatc
     5161 ccatttttac tcctccttct ttctttccca aaatacgact aaactgcaaa gcccgcagag
     5221 acccaaggga ttcatcttgc aaaaaaggaa aaatgagaaa aacggtttat tccccagtag
     5281 tctctgtagt ttctgcggtt tatatttttt ctttaatccg caatttttca gacctctccc
     5341 ctcaccttcg cgctcatcaa tcgatccgct ccccccaagg ataacacttc gttagtcccg
     5401 tcttcaatca tcgccgcccg ggtatcccgg aaaatctttt ccacatagta atccttgcag
     5461 aggccgtatc ccccgtagac ctggatcgcc tcgctggcca cccgaaaagc cgtctcggtg
     5521 gaaaggacct tggccgccat ggcgtagtgg accgccggaa gctgcagttt cttcaggaga
     5581 cctccattgt aaacggctac ccgccgggcg agtgagcgtg ctgcttcaac cgatacaaac
     5641 atgtcaaaga gcttcagttt cacagcctga tgcttgcaga tcggctgccc gccctgcacc
     5701 ctcgtcctgg catattttat ggcctcttcg agagcagcct gggccgttcc ggcaaagcaa
     5761 agccccatca gtccgttggc cagcgccagc tgggcatggg acatgaactt gaagcccatg
     5821 ggttcctggg caatcatcat gcttttggga acgcgcacgt tgtcgaaaaa gatttctccc
     5881 tggttcaacg cccgctgccc gagcttattc agaggttttc cccggaagac ccccggcagg
     5941 ttgaggggaa cgaccgcgat tcctccccct tccatgccga tggatggatt tagggatacc
     6001 cagagggccg catgagtggc aatggagccg ttgctaaccc acgaagactt ctggccattg
     6061 atgaggtagt ggtcgccgtc cgggacggcc ctgacttggg ggacgatctt ggggtttttt
     6121 ccttcttcaa cgtccaggag aatccagtcc gacccatggt ccggttcggt tatcgcccag
     6181 cacccaatca tcctcgcatc tgtatcttcg cagaattttc gggtcaggtc gcgcacttca
     6241 gactccggag aaagctgggc ataggcaaaa ggagtgaccg atacagccag gctcacggcc
     6301 aggtcaggag cggcccagcc catctcctcg ctgatcaggg acatactcag gatatccagc
     6361 tccagccccc cgacggcttt ggggaaaggc attttgtggt agcccagctc ttgggttctg
     6421 cggaatacgt cccagaggat ggaattatgg gcaatcactt cttggggatc ggaaagctta
     6481 tccagcgcga tggaggcggg cctccagact ccctccaaaa atttccgcgc tgcatcccgc
     6541 agggcgatat gctcctcggt caggtccaca ttcaattccc gaatccccat gactttctcc
     6601 tatcgggatc tttatcggac tttctcataa atcctgggca tgtttcggaa acccacttct
     6661 ttgggtggat gacatcatcg atggtcggca ttttcctctc ctctctgatg ctgggaggag
     6721 gaaaaccaaa aagctccaag gaaatgcaaa atcctccttg gagccttcac ctggataccc
     6781 ctctgcaaat tgattttcat gacctacgtc agagagttaa gggttgggct gttgggctga
     6841 gtcagaacgg gtggtgaata cgatgaatga aaattaaagg aaaaaagaga acttgtcaag
     6901 cagaaagaat tcaactcccg gacttgttgc aggggaagac tccttttgac tcccgtctgt
     6961 tgccgacgag ttcattcctt gattttcctg gattcgaata atttgacgat ttccgtcttt
     7021 ccctcctttt ttgctttgac ataaggagtc agcccgtctc tccctttgag gttcgttttc
     7081 gctccttcat ccagaagtag ttttatggct tccatattat tggcccagac ggcatcgtga
     7141 aggggggtgt aaccattttg ggtcccctgg gcattgatat cgaagtgatg ctgcaaaagg
     7201 agtttcacaa tctccatgtt tttctggaac atggcggcgt ggagcgccgt ccctccgaag
     7261 gaatctcgct catccggttt tacccctttt tggagaagtt ccctcacttt gtctatatcc
     7321 ccgtgatagg ctgcctggtg aatttccgcg ccttgaccaa aggcgaaatt agacaggaaa
     7381 agaaagaata aaaggaacgt cgctttcctc atatttctaa cccctttcct cccaagaaga
     7441 ggtgcgagaa atgtttttgg ccggggaaag gatgagaagt ttacagataa tcatagtaat
     7501 tggtgcggat gtaggcgaaa ttgcgctgga tatttttctt tcccttgatg acctctccat
     7561 gccccgccaa gagccattcg gcgtccagct tcgccaagcg ttcgatggac tcccgcagca
     7621 gcatcccatc ccctcccgga aaatcggtcc ggccgacccc catggggaat accacatcac
     7681 cggtgaatag agcctttctc tccggccagt agatgctaat ggacccaggg gaatggccgg
     7741 gggtgtgaag gatttggaag gtcttggagc ccacttttag ctccccttcc ttgaggtaaa
     7801 agcttatctt cagctcgggc atctccccgc ccatggcgcg gtaaaattga ctgccgattt
     7861 cattcaggaa tttctcttcc tcctcgtgga tggcaacctg aaccccgccg cgagcgaaag
     7921 tttgggtacc ctcaaaatgg tccggatggc cgtgggtggc gatgatgagg cggatgtctt
     7981 caaaccggtt cccgtctttc tccatctgtt tgatgagatt cttcaccaga tgctgatggc
     8041 ccgggtcgat gattatcggg atgtctcccc cgatgacgta gctgttacag ttgttctcca
     8101 tggagctctt ccaggtatag gcatagaggt cggaatcgac tttcataacc atcccttcct
     8161 tagaggtgaa tcctcaatca gttggtctgt ggatcctgcc cttttggttc tttcattccg
     8221 aattccgatt ttatattatt atcttttcgt cgctttttcc agaagttcct cgatggtcgg
     8281 gggcaagagg ggaaggggat tcaaggggcg gctattgtga cgcagttcga aatgcaagtg
     8341 cggtccggtg gaccttcccg tgctgcctac ccccccgatc gattgcccct ggcgcacggc
     8401 ctccccttca ttggccatga tcacgttgag atggccatag atggttaaaa agccctgatc
     8461 atgccggagg aaaacgacct tgccgtagcc cgtctgggta ttcctggcca gaatcacctc
     8521 cccgtccatg gccgctttca cctcctggta ggagggagaa cggatgtcaa tcccgccgtg
     8581 gaatcttttt cccctgggcc cgaagggaga ggtaattttc ccctggatgg gccagataat
     8641 gtccagctct tttccccacc agggaggctt ttccgagtct tgcgtcgcct tgttgtactc
     8701 tgaaaggggc ggaagaggag cgggtggttc ccctgtatcc aaggattctt ccaacttctt
     8761 tttctccgat ggggaaaggg gaatataggg ttccaccggc agaaccgcct tggcccccgg
     8821 gatgaacaat ttctggccga ccttgaccgc cgacggagaa gagagcttgt tggcctccat
     8881 cagcctggca atggggactt tatacgcctg gctgatcctg tacagggtga ctcccttggt
     8941 cacaatgtgg taaacgccct cctttttccg ggcgattcct ggcgggctgg agagaaacgt
     9001 gaaaagagag aagaaaaaca cgaagacaac aaaccctctg ccgcagcgga atattctggg
     9061 gggagtgaaa atgatctccg gaaacttttt ctccatcttg agaaaataaa ataacagggg
     9121 aaagagagga atgcaactcc ataaaaagat cgacaaaggg caggaggtca ggttcccaga
     9181 taggccaggc gtatggtctc gctggaaagc agctcagaag ccgagccgtg cagggttatc
     9241 cgacctgtct ctagaacaaa accatgatgg gcgatgctca gggccatttc cgcattttgt
     9301 tccaccagga gaatggtcac ccccgaatgg ttgatctccc cgatgatctt ggcaatctcc
     9361 tcgaccatga gcggagcgag cccgagggaa ggctcgtcca gaagaatgag cttgggccga
     9421 gccatgaggc cccgcccaat ggccagcatc tgctgctccc ctccgctcat gctcccggct
     9481 tcctgcctcc tgcgctcgcg gaggacgggg aaatgctcaa agattctttc caggtcctgc
     9541 tgcaaagcag ctctggagtc tgaacggagg taagcgccca cttccaggtt ttccaggacg
     9601 ctcatacggg gaaaaagctt ccggccctcc gggacctgga caatccccaa ggcgactacc
     9661 tcccggggag tgctccgatg gatgggctct ccctggaaga ggacctcccc cgataagggg
     9721 cgaagcagtc cggatatggt ctttaaggtg gtggacttgc cggcaccgtt ggctcctagg
     9781 agagcgacga tagtcccttc cactacttcc aggctggctc cccggagagc ttcgatcctc
     9841 ccaatgcgta ccactaaatc acgaacgttg agcagcatag cgtttagttc caaggtaagc
     9901 cttgatcact tccgggtgcc ggctcacttc ggcaaaggtt ccttccgcga tcttgcggcc
     9961 gaagttgagc accgcgatct tttcacagag ttccatcaca attcggacat tgtgttccac
    10021 cagcatgatg gtgatctttt cctcccggac ctgctggatc cgggccatca tctcacggct
    10081 ttcctccgcg gagagcccgg aaaagggctc gtccagcaga agcatcttcg ggctgctggc
    10141 cagggcaaca gccagttcca ggattcgctg gtgtccgagg ggaagattcc gggctacctc
    10201 atccctcagc cgatcgatcc ccaggaaacg caaaaggtcc aaagcccgtt cccgctgggc
    10261 cctctcggtc tctccggccc gccgggtatt ccatagcgtc gccaggggat tgatccggaa
    10321 aactccgtag gtgcccagaa gaacattctc caagacagtg aaagactgga agagggcggt
    10381 aatctgaaaa gtccgagcca gtcccaggcg ggggagggca taagaaggca ttccggtgat
    10441 atcggccccg gaaaaatgga cttttccacg ggtggggggc aggaccccgg aaatcaggtt
    10501 gaacaccgta gtctttcccg ccccgttggg gccgatcaac ccaaaaaact cccccggccg
    10561 caccgcaagg ttgaaatcag agatggcggc caacccccca aagtgacggg tcaatccttg
    10621 gacctccaga atgtgattaa agactttccc cttttcctca aacacgagcg gaagcttcct
    10681 tccttttctt gaaccacccc gaaaaatacg ggcccagaga aaccaaccct cctggggaaa
    10741 aaagcatcac caccgccagc atcgtcccat aaacaaccga ctgcaggggg ccgacggccc
    10801 ggatgaactc ggaaaggaca gtcaggacca cgcttcccag gatcggcccg gcaaaagcgg
    10861 ccaccccgcc cacaatcatg taaatctggg tgtagatgga ttgccagatg gtgaagtccg
    10921 tcggagagat aaacccgatg taatgggcat acaccgctcc cgccaccccg gcaaagaatg
    10981 aactgacggc gaaagcgaag agcttgtaat gggtggtctg gattcccacg cacctggacc
    11041 tcagctccgt atcccggatg gccagccaga ttctccccac tcgggattgc tcgaagcgga
    11101 agagaatgat caagctgagc cataataaga tcgaagcaaa ataggcatag gccaatttcc
    11161 cggcgggaat tccttcggga cggggaattc ccaggagtcc gcttggccct cccgtgaact
    11221 cgcgccagcg ggtcgcaatg aggaagaaga cctcgttgaa agcaaaagtg accaagacga
    11281 aatagatccc cgtaagccgc aaggccgggt atcctaagac cacggaaaca accaccgaca
    11341 gcccaccccc ggccagcatt cccagccacg gactcaggcc gatttccttg gccaggaggg
    11401 cggaggtata ggctccgatc cccatgaagg ccgcatgtcc gcaggagaga aggcccacgt
    11461 tccacagcac ccgcaggctc gccgccagaa tgatgttaag gcaggtcatg atgtaaacat
    11521 gaagccagta ggggtctccg atcaggatcg ggagaccggc aaggaccgcg gcgacaataa
    11581 ggaagggagg aagactcatt tcacatccga tacggcaaga ggcccgatgg tctcaccagg
    11641 aggataccga cgaggattac aaaactgaag atgctggcga tcgtaggatc aaagatgctt
    11701 ccggcaatgc tttcgatgaa cccgatgatg aacccccctg ccagggttcc ggtcacgctc
    11761 cccatgcctc ccaggacgat aatgaccagt cctttgagca agggggtgtg gccgatgttg
    11821 gggtcgatca ccagcatggg agaaaccaag aaggcggcgg cggcgcttaa ggcaaagctg
    11881 accacgaaga ccagacggca tatcttggtg atctcgatcc cgtggaggat cgccgcctcc
    11941 cggtcctgct ccaccgcccg gatggctttc cccgctttcg tgtgacggat aaagataaaa
    12001 aggcccacga tcaataaaag gcccccgacg atgaccacca ggcgttcgta agatatggag
    12061 gcgccccaga atttcaacag cccccggaag ggcgaggaga ccgccttgga gatgatgcca
    12121 aagcgcagcc agacggctcc ctcgatggcc agaagaagtc ccaaagacat gaccaggctg
    12181 ggaaggaact gcccgaggag acggcggaag agaaatcgtt ccatcaaaat cccgaggccg
    12241 gccgtgccgg cgatggccag agcccccgaa gaaaaatagt ccaggcccag ttcctcgcag
    12301 aggatgtaga gtagcatggc cgaaagcatg tacaccgttc cctgggcgaa gttcacgatg
    12361 tgcatgatcc ccatgatgag gttcaatccc agtgccacga ggatgtagta acaggagaga
    12421 accactccgt tggtgaagac ctgcatgtaa aaaggaaagg agtccattac cattctctga
    12481 aatcccccca ctcctctttg cagggagact gtatcgtaag tccgttcatg gttcgacagg
    12541 cctgtcctga gcgaagtcga agggctcacc acgaacggac cacggcacat ttgaagtcaa
    12601 ttgcttagcc gttcgtcgtg agtccgtcga ttcctcattt gtcctgagcc catcgactta
    12661 ccgttccccc tgagccttcg gcgtgagctc agtcgaacgc ttgtcgaagg gtgaggggtt
    12721 cgccaaggac aggcgtgccg aaggacgaat ggcgaattgc cgcacagtct tgagggtcag
    12781 aggggattta tacaaggtct tttcagcttt taataacctt ccggcatgcc cccccggtga
    12841 agttccacta gctttttctg cttggtgtca taggtggtca gaggcaccgg gaagaggaat
    12901 tggcagtcaa tgccgtaata agattttccg ccgatcctcg tgcgcccaaa tactccgtcg
    12961 atttgcacct tgggcatggc atcccttacc gccgtggagt ccacacttcc agccatctcg
    13021 atggcctttt tgagtacata gcctacatcc gaatttctcc agacaatcgg gtcgaatttc
    13081 tgtttatatt ttccctcata ctgtttggcc accttggtgg cgatcgggct aggatcggtg
    13141 gcataaatgc cgtagaagag gaccccgtcc atgaacccgg ccgccacttt ttcaaaggtg
    13201 gggatggatt gggagtagct gaagatcatc accccggtat accccagctc tttggcttgt
    13261 ttgcaggcca ggggtccgtc cgaaccggcc caggctccga agtcgatggc ctgaggtttc
    13321 ttggcgatca gtttggtcag catgggggtg aaatcggcgg ttccgcgggg agcgaaggat
    13381 ttgtcggtaa tggccaggcc gagcttcttg gccacaattt ccgcatcctc ggcctggatc
    13441 aatccggtct cgtcgtccgt cgaggtgagg gcgatagtct tcaccttaaa tttttccaca
    13501 atatagggga ggagggcgga gtggccctgg atcggctcca tgatggaccg gaaggtatag
    13561 ggggtgctgg gattggtggc ctttttcccc ccataggcgt agccgatgtg gaggactttg
    13621 ttctcattta tcagcgggtt gacggcaata acacaagtcc ctccggtggt gacgatatac
    13681 ttcaccttat cattatagat aagcttattg accgaagcga ccgcttcttt ggcgtcgtat
    13741 ttgttgtcat aggtgatgag cttgaaggta taggtttcct tcccgacctt gatcccaccg
    13801 gcgccgttta tgtcctcggc ggcaagttcc accgcccgca gaagcccgag ccctgtgggg
    13861 gcagcggctc cggacaaagg ggtgttgatc ccgatcacca ggtctttcgc cagcccgggg
    13921 ttgaaagcaa aaggaatgac caccagggag atgctcaaag cgaggataga gggacgaaac
    13981 aattttccca tggcacaacc tccttttgat gaatgaaatc cttggaaaag cctcacgggg
    14041 tataaagagc ctcaggaaag ggcacgcaag gctcccgaaa ccgcagtgat aaagtcctcc
    14101 acccgggccc cggccacgat cattttctct ttttcccatt tttggcgaat gatcttctcg
    14161 atttcggcct ccatggcggg tgcggaacgg agggcttgtt cgatctcctc cggaatctcc
    14221 cttcgggaag gcatcatgtt tccggtaatc aggacgtcga gaacctggtc ttcaaggacg
    14281 gcgaggttga cccgcaagat gggcccaccg gagactttgt aaacaaactg cgaaaatttc
    14341 gccctcggag gaagctgggc aaagcggcgc cgggttgatt tctggaagaa ccattcatcg
    14401 ttatcgtaca tccggtggta ggactgccgg aattctttct ccgcctccgt gatctccccg
    14461 ggtgctaggg tcacatcaaa
//
LOCUS       DASPRR010000507         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1896830, whole genome shotgun sequence.
ACCESSION   DASPRR010000507 DASPRR010000000
VERSION     DASPRR010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896830"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VEL68_18680"
     CDS             complement(<1..916)
                     /locus_tag="VEL68_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HXZ38057.1"
                     /translation="MPLMTTKPMFDLAYDGGFAVGAFNVNNMEITQGIITACAAEKAP
                     LILQISKGARKYANMRFLKHIIDAAVEENPDLPIVIHLDHGDTVDLVDACIKDGFTSV
                     MIDGSHSPFEENVKLSAAAARAGHQKGVVVEAELGMLGGIEEHVVGLSAEEYEKNLGK
                     FLTDPKQAADFWKQTGVDSLAIAIGTSHGAYKFKHEAKLAIGRIEQIMKTCPGLPLVM
                     HGSSSVPQEYVDLINKYGGNMPNARGVPEDQINLAVTKYGVCKVNIDTDLRLALTAKI
                     REVFATKPSEFDPRNYLGPGRDAIVKTVK"
     gene            1180..2400
                     /locus_tag="VEL68_18685"
     CDS             1180..2400
                     /locus_tag="VEL68_18685"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ38058.1"
                     /translation="MDIEKRKIPTGPPPLKGTRVIDLTRIIAGPYCAMVLGDLGAEVI
                     KVEQPKIGDESRAWGPPWVKEVSAYFIAINRNKKSLTLNFKHPQGVEIFKNLVRHSDV
                     LLENFRPGTMDELGIGYEVLREINPKLIYCDMTGYGTEGPYRDKPGVDVIVSAIGGLM
                     SITGPPEGEPVKVGVPLTDVLTGLYSFGAVSSALYQREHTGKGQRISINLLAMQLATL
                     INIGSNYLVGEVIPRRWGSAHPNIVPYEAHRAKDAYLIFGVVNERMWKSFCTLLGMEE
                     MKDDPRFAENAARIKNRPALMEIIDRKLAEKTVDEWVPLFDRAGIPCGPINTFDRVFS
                     DPQVQHLGLVQEVDHPHYGKVKIVGPPATFSESAVGIQCPPPLLGEHNQEILTRLLGY
                     SEDQAQTFKDQGVI"
     gene            2675..>3941
                     /locus_tag="VEL68_18690"
     CDS             2675..>3941
                     /locus_tag="VEL68_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXZ38059.1"
                     /translation="MIYTFKYFSLFLVVIFLAGACTKVGPNFARPETEVSLRWMEAED
                     KRLIHDPADYRNWWRVFNDPVLNGLIERAYRDNLSLRIAGVRVLEARAQLGIAVGEFY
                     PQTQQVSGSLQYNRVSERSVAGLALSQFNYAQGQIGVGASWELDFWGKFRRAIESANA
                     AWLATVADYDSVLVSLTADVANTYVLIRTLEKRTEIARENVEAQKEALKIAEARFEHG
                     TTSRLDVEQARTVLNNTLASIPALESQLQQAKNALCLLLGLPPSDLSELLAREKGIPI
                     SPLQVAVGIPADLLRRRPDIRSAERQAEAQSALIGVSKADLYPAFSLSGFFGFLSTNV
                     GNFSLGDIFQWKSRTFQIGPSVQWNFLNYGQITNNVRVQDARLEQLLIAYQNAVLKAQ
                     QEVEDALVAFLRAQERAEFLAWSTASAKNS"
BASE COUNT          823 a         1106 c         1184 g          828 t
ORIGIN      
        1 gcttgaccgt tttgacgatg gcgtcgcggc cgggccccag gtagttgcga gggtcgaatt
       61 ccgaaggttt ggtggcaaag acctcacgga tcttggcggt gagagccagg cgcaggtcgg
      121 tgtcgatgtt gaccttgcag actccgtatt tggtcaccgc caggttgatc tgatcctccg
      181 gcacgcccct ggcgttgggc atattcccgc cgtatttgtt gatcaggtcc acatactcct
      241 ggggcacgct ggaggagccg tgcatgacca ggggaaggcc cgggcaggtc ttcatgatct
      301 gttcgatccg gccgatggcc agtttggctt cgtgcttgaa tttgtaggct ccgtggctgg
      361 ttccgatggc gatggccagg ctgtccacgc cggtctgctt ccagaagtcg gcggcctgtt
      421 tcgggtcggt caggaatttt cccaggtttt tttcgtactc ttcggcgctg agccccacga
      481 cgtgctcttc gattcctccg agcatcccca attcggcctc caccaccacg cccttctgat
      541 gccccgccct ggcggcggcg gcgctcagct tcacattctc ctcgaagggg gagtgagacc
      601 cgtcgatcat cacgctggtg aagccgtcct tgatgcaggc atccaccagg tccaccgtgt
      661 cgccgtggtc caggtggatg acaatgggga ggtccgggtt ttcctccacc gccgcgtcga
      721 tgatgtgttt caggaaccgc atgttggcat atttccgggc ccctttggag atctgcagga
      781 tcaggggggc tttttccgcc gcgcaggcgg tgatgatccc ctgggtgatc tccatgttgt
      841 tcacgttgaa ggccccgacg gcaaatccgc cgtcataggc caagtcgaac atgggtttgg
      901 tggtcatgag aggcatagaa ggactccttg gggtggaatt aggacaattc tagctcagta
      961 tagtgcggtg ggggaaaaaa gtcaaaaaag gggagggcca tcatagtttg aggagataag
     1021 gaacgaagag atgagggaaa tgaaaaaggc tgggtcgatg atgggatggg gagattagag
     1081 gaggcgcgcg gtgaaagtct tttcggttga tagccaagag tggatatgct aataaaaatt
     1141 atttggaatt tggccagagc attgagagaa ggtgaattta tggatataga aaaaagaaag
     1201 attccgaccg gtcctccacc ccttaaaggg acccgggtca tcgatttaac gaggatcatc
     1261 gccggccctt actgtgccat ggtcctgggg gatttgggag cggaggtcat caaggtcgaa
     1321 cagcccaaaa tcggggacga gagccgcgcc tggggcccgc cctgggtgaa ggaggtcagc
     1381 gcttatttca tcgccatcaa ccgaaacaag aagagcctta ccctgaattt caagcatccc
     1441 cagggggttg aaatcttcaa aaacctcgtc cggcattccg acgttctcct ggagaatttt
     1501 cgtcctggca ccatggatga actgggaatt gggtatgagg ttctgcggga aatcaacccc
     1561 aagctgatct attgcgacat gaccggatac ggcacggagg gcccttaccg ggacaagccg
     1621 ggggtcgatg tgattgtctc agccatcggg ggtctcatgt ccatcaccgg cccgccagag
     1681 ggcgagccgg tgaaggtcgg ggtcccgctt acggacgttc tgacgggatt gtattctttc
     1741 ggagctgtat cttcggctct ttatcaacgg gagcatacgg gaaaaggcca gagaatctcc
     1801 atcaaccttt tggccatgca gctggccacg ctcatcaaca tcgggtccaa ctacctggtg
     1861 ggggaagtga tcccccgccg ctgggggagc gcccatccca acatcgtccc ctatgaagcc
     1921 caccgggcga aggacgccta ccttatcttc ggggtggtca acgagaggat gtggaagtcc
     1981 ttctgcaccc tcctgggaat ggaagagatg aaggacgacc cccggtttgc ggagaatgcc
     2041 gcccggataa aaaaccggcc cgccctgatg gaaatcatcg accgcaaact ggccgaaaag
     2101 accgtggacg aatgggttcc cctcttcgac cgggcaggga ttccctgcgg acccatcaac
     2161 accttcgacc gggttttcag cgaccctcag gtccaacacc tggggctcgt gcaggaggtg
     2221 gatcatcctc actacggaaa ggtaaagata gtgggtcctc cggccacctt ttccgagagc
     2281 gcggtgggaa tccaatgccc cccgcccctg ctgggagaac acaaccagga aatattgacc
     2341 cgccttctgg gatactcgga agatcaagcc cagacattca aagatcaggg ggtcatttaa
     2401 cccccgcctt tcccgtccgg ttgccggacg gcccgtgggg acacgatgcc tggtgccctc
     2461 actgcagttt tatatactgt cttttctccc gcaaaatact gtgtttccct cattccaaag
     2521 accactcatt ccaaaagaga attaagtcat cgggaccggt tcttctcaac cttcgatttc
     2581 cgtgggtaaa accgaaggag ggggagtgac tgggtgcgat tccttctgag ccctgcctca
     2641 ggaatggggg cgcgggctgc acaaaaggcc tcctatgatc tacacgttta aatatttttc
     2701 tctcttcctc gtggttattt ttctggcagg cgcttgcacg aaggtagggc cgaacttcgc
     2761 tcgccccgag actgaagtct ctttgagatg gatggaggcg gaagacaaaa gattaatcca
     2821 tgacccggcg gactaccgca actggtggcg ggtattcaat gatccggtcc tgaacgggct
     2881 catcgaaagg gcgtaccggg ataacctctc cctccggatt gccggcgtgc gggtgttgga
     2941 ggcccgggcc caactgggca tcgcggtggg agagttctac ccccagacgc agcaggtgtc
     3001 aggctctctt cagtacaacc gggtcagcga gcggtctgtc gcaggcctcg ccttatcgca
     3061 gttcaactac gcccagggcc aaatcggagt gggcgccagc tgggaactcg acttctgggg
     3121 gaagttccgg cgggcgatcg agtcggccaa tgcggcctgg ctggcaacgg tcgccgacta
     3181 cgacagcgtc ctggtgagcc ttacggcgga cgtggccaat acctacgttc tgatccggac
     3241 gctggaaaag cggaccgaaa ttgccaggga gaacgtggaa gcccagaaag aagcgctgaa
     3301 aatcgccgag gcccggtttg aacatgggac cacctcgagg ctggatgtgg aacaagcccg
     3361 gacagttctc aacaacaccc tggcttccat cccggccctg gaaagccagc tgcagcaggc
     3421 caagaacgcc ctctgcctgt tgctcgggtt gccacccagc gatctaagcg agctgctggc
     3481 cagggagaag ggtatcccca tctccccgct ccaagtggcg gtcggtatcc cggccgatct
     3541 gcttcgccgg cgtccggata tccgcagcgc cgagcggcaa gccgaggccc aaagcgccct
     3601 gatcggcgtg tccaaagcgg acctctaccc ggccttttcc ctcagcggct ttttcggctt
     3661 tctctcgacc aatgtgggga atttcagcct cggcgacatc tttcaatgga agagccgcac
     3721 cttccagatc gggccttccg tccagtggaa cttcctcaat tatggccaga tcacgaacaa
     3781 cgtccgggtg caagatgccc ggctggagca gctgctgatc gcctaccaga atgcagttct
     3841 caaagcccag caggaagttg aggatgccct ggtggctttt ctgcgggccc aggaacgggc
     3901 cgagttcctt gcctggagca cagcctccgc caagaactct t
//
LOCUS       DASPRR010000508         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2060735, whole genome shotgun sequence.
ACCESSION   DASPRR010000508 DASPRR010000000
VERSION     DASPRR010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060735"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1120)
                     /locus_tag="VEL68_18695"
     CDS             complement(170..1120)
                     /locus_tag="VEL68_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HXZ38060.1"
                     /translation="MAVRMGTGKFTYEAHPDWAKLPKGWRFLEVVDAAVDSRDRVYVF
                     SRGEHPLMIFDREGNFLTSWGEGRFKRPHGITVGPNDTLYLADDGGHAIYQFTVEGEL
                     LMTIGTPGKAAEFMGGEPFNQPTKAARDPKTGELFISDGYGNARVHRYTSQGKYLSSW
                     GGPGIEPGQFNLPHSVALDKDGRVFVADRENLRIQVFDQQGKFLTAWNNIHRPCGMHI
                     AGVEEQLVYVGELAPAYPFNQKYPNLGACISIYDLKGNRLARLGDSRPGEEPHQFLAP
                     HGMTVDSRGDIYVGEVSWSFKGKTLTPPRELPSFRKLVKV"
     gene            complement(1309..1908)
                     /locus_tag="VEL68_18700"
     CDS             complement(1309..1908)
                     /locus_tag="VEL68_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38061.1"
                     /translation="MKKVLALCAVLLVGLILGAGCSQDKEPAEAALKAAEAALSAAKA
                     EAMKYVPDQVKGVEDALKTAKEAFEKKEYTQALNAAKDLPAKAKELADAAAAKKAELT
                     KSWEEMAAGLPKMVEAIKSRVDILSKSKKLPKGMDKPKLEAAKAGLGEITQGWTDAEN
                     AFKGGNIADALAKGNAVKAKATEIMTTLGMQPPAAAKAS"
     gene            complement(1928..2125)
                     /locus_tag="VEL68_18705"
     CDS             complement(1928..2125)
                     /locus_tag="VEL68_18705"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXZ38062.1"
                     /translation="MAEEKTGGDAWDSTQWHVVKKGESLWKIAEQYYGDGSLYKKIFE
                     ANQDILSDPNLIKVGQKLKIP"
BASE COUNT          513 a          708 c          627 g          640 t
ORIGIN      
        1 ggtatcaatc acacctcact cttgcctagc ccccttgaaa atgcccacga ccgcacgggc
       61 gattcatgaa tctacccctg ttacaagcct tgcggacaat tttcaatcct caatatcatt
      121 aaattctacc gcatctaggc cgggccgaca tgagcaaagt cattcatttt tagaccttga
      181 ccagcttacg gaagctgggc agttctcggg gcggggtgag ggtcttgcct ttaaaactcc
      241 aggagacctc tcccacgtaa atatctcctc gtgaatcaac ggtcatgcca tggggggcca
      301 gaaactggtg gggttcttct cccgggcggg aatcccccag ccgggccagg cggttaccct
      361 taaggtcata aatactgatg caggctccga gattggggta tttctggtta aaggggtagg
      421 cgggcgcaag ctctcccaca taaaccaatt gttcttcgac tccggcgata tgcatgccgc
      481 agggtcggtg aatgttgttc cacgcagtca gaaatttacc ctgctggtca aagacctgga
      541 tacggaggtt ctcccggtcg gccacgaaaa ctcgcccatc cttatccagg gctacgctat
      601 gcggtaggtt gaattgcccg ggttctatcc cgggtcctcc ccaggaggac agatatttac
      661 cctgcgaagt atatctgtga accctggcgt tgccgtatcc gtcggaaata aaaagctcac
      721 cggttttcgg gtcccgggca gccttagtcg gctggttgaa gggctctcct cccatgaatt
      781 cggctgcctt cccgggagtc ccgatggtca ttaacaattc tccctccacg gtgaactgat
      841 agatcgcatg gccgccgtca tcggccagat agagggtatc gttcgggccg acggtgatcc
      901 cgtggggcct tttgaaccgg ccttcccccc aggatgtgag aaaatttccc tcccggtcaa
      961 agatcatgag cgggtgttct cctcggctga aaacatagac ccggtcccgg gagtccaccg
     1021 ccgcgtccac aacctccaga aacctccacc ccttgggaag cttggcccaa tcgggatggg
     1081 cctcataagt aaattttccg gtacccattc tgacagccat tttttctcct tctcatcgct
     1141 atgagaattc ctgttccgtc atggcaggat atcgcggctt tctgattaga cagcaggact
     1201 tgagattcta ccggaaaaag aaagggggat cttgcgatcc ccccgtcatt tttcggttgt
     1261 tcgctcaggg attcacacca ctttggaaag cggattccga ggataccctt aggaggcctt
     1321 ggcagcggcc gggggctgca tgcccaatgt cgtcatgatc tcggtggctt tagccttcac
     1381 tgcgttgccc ttggctagag cgtcggcgat gttcccgccc ttaaaggcgt tttccgcatc
     1441 cgtccagccc tgggtgattt ctcccaaacc agccttggcg gcctccaatt tgggtttgtc
     1501 catccccttg gggagtttct tgctcttgga gaggatgtcc actcggctct tgatggcctc
     1561 gaccatcttg ggaaggccgg ccgccatctc ttcccaactc ttggtcaact ccgctttttt
     1621 ggctgcggcc gcatcggcca attctttggc cttggcggga aggtctttgg ccgcattcag
     1681 agcctgggtg tattctttct tctcgaaagc ctctttggcg gtcttcaggg cgtcttcaac
     1741 acccttgacc tgatcgggca catacttcat cgcctcagcc ttggctgcac tcaaggccgc
     1801 ctccgcggct ttaagtgccg cttccgccgg ctccttgtct tgggagcacc cagctcccaa
     1861 aatcagtccc acaagcaaga ccgcacataa cgcaagaacc ttcttcatga tcgctcctcc
     1921 tttttattta tggaatttta agtttctgac ccaccttgat caggttgggg tcggaaagaa
     1981 tatcctggtt ggcttcaaaa atttttttgt aaaggctccc atccccataa tattgctcgg
     2041 caatcttcca aagggactcc cctttcttca ccacgtgcca ctgggtcgaa tcccacgcat
     2101 cgccccctgt tttttcctcg gccatatttt gcctcccttt gaagatggat atcaagaatc
     2161 ccccgtactt ctttcgggct atggggaaac cgtttcgacg aacatgggag aaagagattt
     2221 tgtttcttcc tcagcaccaa gggacgagat cgtcaccctg gaaagcaaag agggaattaa
     2281 agtctcagct taataagatt ctattggacc caatgaattc cgtcaagggg atttcatctc
     2341 ttgcccaccc ccggttctta aaaaagagtc cttcgaaaat aagatctctg gaaaggaggg
     2401 gggatctcat ggggttcagg gcggagaaat ttggattatc atcctgaggc ggtggttctt
     2461 ccatgagatc gggcctgtcc tgggggga
//
LOCUS       DASPRR010000509         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1738865, whole genome shotgun sequence.
ACCESSION   DASPRR010000509 DASPRR010000000
VERSION     DASPRR010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738865"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VEL68_18710"
     CDS             complement(<1..513)
                     /locus_tag="VEL68_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38063.1"
                     /translation="MSAKRLHRWLLGWFAVLMVVQPQISLAEPLDHWQLKAPLTQWHS
                     IRISYGNGIFVAVGEPGALYTSSDGETWTWRSSGTNQSLRDVSYGGGTFVAVGKGGTI
                     LSSPDGVIWTGCNSGIGHDLNGVSYGENTFVAVGDGGAILASSDGLTWAARDSGTHQG
                     LNKITYGKDTF"
     gene            complement(621..2591)
                     /locus_tag="VEL68_18715"
     CDS             complement(621..2591)
                     /locus_tag="VEL68_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HXZ38064.1"
                     /translation="MTEERNPSAPEGVSPSALEKAGEIVDRYEGLTRKLAGLSGWIIT
                     AVAVGTSVFYLYSATATLVTQMLRGLFVMLTLFLTMTAFPATRGTRHRIPWYDWVLAF
                     LSCAPILYMLWDFEEFVYRAVTPTQMDVIMGVILIVLVLEAMRRTTGWILSVIVLGFL
                     VYAYMGEYLPSPWNHRGYDIERIVGHMYMALEGIFGVPIDVAATFIILFTIYGAFLDK
                     SGAGQFFIDFSFAAMGGKPTGAGRTAILASFLLGGPAGSGVATTVTIGSVAWPMLRKA
                     GYDRESAGGFVSAGGLGAVLSPPVMGAAAFLICEILKISYLEVMAMACMPTILYYWSI
                     FLMVEFDAKKFGAKEVIVERKGSLKELTFRYGYHFISLITIVLFMLLGFTAILAVFWS
                     IVIVFALSFIRPESALGPARLVEALRKGTMDVLSVAATCAGAGIIVGIVTLTGLGLQF
                     SGIVIDLAGGNLFVTILFTAFALWVIGLAVPVTGTYIIAVVITAPAMIKLGVPEYAAH
                     MFIFYYAVLAEVTPPTALSAFAAAAITGGDPYKTTMMSWKYTLPAFLVPFMFTIHPDG
                     VGLLLKGSLGNIILTNLTALIGVGALAAGMSGWLIRKTDWVERPLLLAGGLILVYPSP
                     AADILGVGLVAAVALRQWRTKKKDLGAKPPGI"
     gene            complement(2723..3682)
                     /locus_tag="VEL68_18720"
     CDS             complement(2723..3682)
                     /locus_tag="VEL68_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ38065.1"
                     /translation="MFLQIAGSTLAAALLTPSAPLCAQQRKLSIATGGTGGVFYVMGG
                     GIANLLTKTLPNAKVTAEATAAAVDNCKLIDVRKADLGFSPGDVLYDSYVGQDKFKTK
                     VPVRTLLNPYSNFIHFVASESSGIKSMKDLRGKRVSLGAPGSGTEVKAARILESLGID
                     PTKDIKRDRLSVAESAGGIKDRKLDAFCWSGGLPTAAIMDLAATPGVHVRLLDMAEIV
                     PKLSDKFGPVYFKGLIPKGTYPGVDYDVVTAAMVVSIFCHEKMEEELAYQITKIIIEK
                     KSELVLVHKEAEHISLQDSAVGSPVPYHPGAIRYFKEKGVTPK"
     gene            complement(3794..>4003)
                     /locus_tag="VEL68_18725"
     CDS             complement(3794..>4003)
                     /locus_tag="VEL68_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38066.1"
                     /translation="TTILLVEQNAKMALSVAHFGYVLETGRIVLANTPQNLMEDEDVK
                     EFYLGIKSEVSGKGYQRWRRKKRWR"
BASE COUNT          911 a         1164 c         1134 g          794 t
ORIGIN      
        1 aaaggtgtcc tttccgtagg taattttatt gagcccctga tgggtcccgg aatccctcgc
       61 cgcccaggtc aagccatcgg aagaggcgag gatggctcct ccgtctccca cggccacaaa
      121 agtgttctct ccataactca ccccgttgag atcatgaccg atccccgagt tgcaccccgt
      181 ccaaattact ccatccggag aggaaaggat cgtcccccct ttccccacgg cgacgaaggt
      241 gccaccgccg taggatacat ctctcaggga ttgattggtc cccgaacttc tccatgtcca
      301 ggtttcccca tcggaagagg tatagagcgc cccgggttcc cccacagcca cgaagattcc
      361 attcccataa gatattcgga tggaatgcca ttgggttaga ggagctttca gttgccagtg
      421 atcgagaggc tctgcgaggc taatttgcgg ctgaaccacc atcaggacgg cgaaccatcc
      481 cagtaaccat ctgtgaagcc ttttggcact caaaatagca ccctctcgtc cgggctcccc
      541 cccatggctt tctcatgacc tttttgaagc tatcatcctg gctgggacct taaggaaagg
      601 ggagaaaagg gaccatcggg ttaaatcccc ggcggcttgg cacccaaatc cttttttttc
      661 gtgcgccact gccggagggc aacggcagcc accagcccga cccccaggat atctgcggcc
      721 ggactgggat agacgagaat gaggccgccg gccaggagga gcggacgctc gacccagtcg
      781 gtcttgcgga tcagccagcc gctcataccg gcagccaagg ctcccacccc gatcagggcg
      841 gtcagattgg tcaggatgat gttgcctaat gagcctttca gcaataggcc cactccgtcg
      901 gggtgaatgg tgaacatgaa cgggaccagg aaggcaggta gagtatactt ccacgacatc
      961 atggtggtct tgtaagggtc ccccccggtg atggccgcgg ccgcgaaagc cgaaagtgcg
     1021 gtcggaggag tcacctcggc caggaccgcg taatagaaga tgaacatgtg ggcggcatac
     1081 tcaggaacgc cgagtttgat catggccgga gcagtaataa ccacggcaat gatgtaagta
     1141 ccggtgaccg gaaccgccag tccgatgacc cacagggcaa aggcggtgaa caaaatcgtg
     1201 acgaagagat tccccccagc caggtcgatg acgatcccgg aaaattgcag ccctaatccg
     1261 gtcagggtca cgatgccgac gataatccca gccccggcgc aggtcgcggc aaccgagagc
     1321 acatccatgg tccctttgcg gagggcctcg accagccggg ccgggcccaa ggcgctctcc
     1381 ggccggatga aactcagggc gaagacgatc acgatggacc agaagaccgc cagaatggcg
     1441 gtaaagccca aaagcatgaa tagaacaatc gtaatcagag aaataaagtg atacccgtag
     1501 cggaaggtga gttccttcaa gcttcctttt ctctccacga tcacttcctt ggccccgaac
     1561 ttcttggcgt cgaactccac catgaggaag atggaccagt agtagaggat cgtgggcatg
     1621 caggccatgg ccatgacttc caggtaggaa atcttcagga tttcgcagat gagaaaggcc
     1681 gccgcgccca tgaccggggg agagaggacc gcacccagcc ctcccgccga tacgaaccct
     1741 ccggcggact ccctgtcgta gccggccttg cggagcatgg gccaggccac cgagccgatg
     1801 gtcacggtgg tggccacgcc gctgcccgcc ggcccgccca gaaggaagga ggccagaatg
     1861 gcggtccggc ccgcaccggt gggctttccg cccatggccg caaaggaaaa atcgatgaag
     1921 aactggccag ccccggactt atccagaaac gcgccgtaga tcgtgaatag aataataaag
     1981 gtggcggcca cgtcgatggg aactccgaag ataccctcca gggccatgta catgtggccc
     2041 acgattcgct cgatgtcgta cccccggtgg ttccaggggg aaggaagata ctcccccatg
     2101 taggcataga ccagaaaccc caggacaata accgaaagga tccatccggt ggtccgccgc
     2161 atggcttcca ggaccaggac gatgagaatg actcccatga ttacatccat ctgggtggga
     2221 gtaaccgcgc ggtagacgaa ttcctcaaaa tcccagagca tgtaaaggat cggcgcgcag
     2281 ctcaggaagg ccagaaccca gtcataccag ggaatccgat ggcgggtccc ccgggtggcc
     2341 ggaaaggcag tcatggtaag gaagagggtg agcatgacga agaggccgcg gagcatctgg
     2401 gtgacgaggg tggcggtcgc cgaatacaaa taaaaaacgg aggtgcccac cgcgactgcg
     2461 gtgatgatcc atccggaaag ccctgcgagc ttccgggtca ggccttcgta ccggtctacg
     2521 atctcccctg ctttttccaa tgcgctgggg ctgaccccct cgggcgcgga aggattcctc
     2581 tcttcagtca tggttaagat ttttctcccg ggtgatagca ggaatgaaaa actgacattt
     2641 ctttcagaac gatctcaggg agcggccgga ttcatgcttc cctccccgcc cctctgcccc
     2701 ctcggcctaa atcctgtgag acttacttgg gggttacccc tttttctttg aaatagcgaa
     2761 tggctcccgg gtggtaagga accggagagc cgaccgcgga gtcctgcaag ctgatgtgtt
     2821 cggcttcctt atgaaccaga acgagctcgc tcttcttttc gatgatgatc ttggtgatct
     2881 ggtaggccag ctcttcttcc atcttctcgt ggcagaaaat ggagacgacc atggccgccg
     2941 taactacgtc ataatccacg ccgggatacg tacccttggg aatcaagccc ttgaaatata
     3001 ccggcccgaa tttatcgctc agtttgggaa cgatctccgc catatccagg aggcgaacgt
     3061 ggactccagg cgtcgccgcc aggtccatga tggcggccgt gggcagaccg ccggaccagc
     3121 aaaaggcgtc gagcttccgg tctttaatgc cgcctgccga ttccgccacg ctcaagcggt
     3181 cccgtttgat gtctttggtc gggtcgatgc ccagggattc caaaatccgg gctgccttga
     3241 cctctgttcc gctccctggg gccccgaggg agactctttt cccccggagg tccttcatgg
     3301 atttgatccc ggaactctcc gaggccacga agtgaatgaa attcgaatag ggattcaaaa
     3361 gggtgcgcac cgggaccttg gtcttgaatt tatcctgtcc cacataagaa tcatagagca
     3421 catctccggg gctgaaaccc aagtccgcct tgcgaacgtc gattaacttg cagttatcga
     3481 ccgccgctgc tgtggcttca gccgttacct tggcattggg cagggttttg gtgagcagat
     3541 tggcaatgcc cccacccatg acgtagaaca cgcctcccgt ccctccagtg gcgatggaga
     3601 gcttccgttg ctgggcacac agcggagcgg aaggggttaa cagggcggcg gccagggtgg
     3661 aaccggcaat ttgaagaaac atcctccgtg aaaaagcact ctcctgtatc attgtcctgc
     3721 ctcctctaaa aaagtaatct gcccgatcat gggagggatg tgtatgggga tggatgagag
     3781 gaattcactt tatctatctc cacctttttt ttcgccgcca gcgctggtaa ccctttcccg
     3841 aaacctccga tttgattccc aggtaaaatt ccttaacgtc ctcgtcttcc atgagattct
     3901 ggggggtgtt ggccaggaca attcgacccg tctccagaac atatccgaaa tgagccacgg
     3961 aaagggccat cttggcgttc tgttctacca ggaggatggt tgt
//
LOCUS       DASPRR010000510         9685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1898064, whole genome shotgun sequence.
ACCESSION   DASPRR010000510 DASPRR010000000
VERSION     DASPRR010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898064"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VEL68_18730"
     CDS             <1..230
                     /locus_tag="VEL68_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ38067.1"
                     /translation="EFGNRLSFWGGIDTHRVLPFGTPGEVRREVRKMIDCLGKGGGYL
                     LASVHNIQAEVPPENIAAMFDEAREYGRYQH"
     gene            complement(318..734)
                     /locus_tag="VEL68_18735"
     CDS             complement(318..734)
                     /locus_tag="VEL68_18735"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXZ38068.1"
                     /translation="MLSRNEILKALAQWNRAWDDHDLDGVMALFHENIVFENWTGGRA
                     EGKEALRKAWEGWFKNHGNFRFIGEDTFVDGETQKVLYEWTLDWPSIEKGYQGKREKR
                     RGLDVMHFKDGKIIRKSTYCKTTLEIEGKRVKLAAE"
     gene            840..2195
                     /locus_tag="VEL68_18740"
     CDS             840..2195
                     /locus_tag="VEL68_18740"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXZ38069.1"
                     /translation="MASSQKILTNLKKLVEQSPADQTEALLMTEDSSLTRFTPASVHQ
                     HVAERNQTVILRVVLGKKVAVVTTNLLSPSSLRTALDKAVSLARSLPANPDFRSLPQA
                     RPIPEVNTFFESIRRLTPGKKIKAIQEIMGLVKEKGASVSGAFSHGTVEVAVLNSLGV
                     EAFQQYSDFSLHLIAQNNHSSGYASFVSRDPDRLEIESLARSAVSKISMERPVEISPG
                     EYEVILEPDAAHEFLSFMGFLGFHALAVQEGRSFLGNHFGEKLLDERVTIYDDGLDPH
                     GLQVPFDFEGVPKQKVLFFDKGVARNVTYDSLTAGREKKESTGHALTPPNTEGPIPVN
                     LFMEGGHSSLEGMIRSIRRGIYVTRFHYTNVVEPMKAVITGMTRDGTFWIEDGEVRNP
                     VKNLRFTESILKALSQVKAISRERKICTSGAVYGRRFVTGTVAPALQIDGFYFSGVSS
                     L"
     gene            2294..3736
                     /locus_tag="VEL68_18745"
     CDS             2294..3736
                     /locus_tag="VEL68_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXZ38070.1"
                     /translation="MKGLIYHLLDMAKVRKATYADIRIVHRQMEEISVKNGKVDAVNQ
                     NEDQGFGVRVLFQGVWGFAASSIINKAEMEKTLARAWRIAQVSSRVKEKDVLFSRQAA
                     IMENYRTPASIDPFSISPDRIIDLLLESEKILRRNPKVKVSEAFMGAYKTEKTFASTE
                     GSFIEQEIVECGAGISATAIEGSEVQVRSYPNSFRGNFATRGYEWIEGLKIKDHAERI
                     AEEAVQLLSAKPCPSGTTTLILEGSQLALQVHESIGHPIELDRILGTEASYAGTSFLS
                     LDKVGKFPYASEVVNVTADATIPGGLGTFGFDDEGIRAQRVPILSHGVLSNVLTSRET
                     APLLKKESNGTMRADGWNRIPLIRMTNINLEPGEWTLEGMIADTDEGLFLSTNRSWSI
                     DDKRINFQFGTEIGWEIKKGKLGDMVKNPTYTGITPQFWNSCDAVANRDYWLLWGTPN
                     CGKGEPGQVAHVGHGTAPARFRNVRVGLMK"
     gene            3984..5159
                     /locus_tag="VEL68_18750"
     CDS             3984..5159
                     /locus_tag="VEL68_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXZ38071.1"
                     /translation="MKLPLDGVRVLEMGHTVMGPTCGLVLADMGAEVIKIERAPKGDD
                     TRRLIGFGQGFFLYFNRNKKSLAVDLKSEKGAGLVKRLATSSDVFIENFGPGAVDRLG
                     LGYEDLSKVNPRLIYCSLKGFLPGPYEHRPALDEVVQMMGGLAYMTGPLGRPLRAGSS
                     VVDIMGGSYGVIGILTALYEREKTGRGQFVIASLYESTAFLMGQHMALSAVSGKPVPP
                     MPSRVSTWGIYDIFKTQDGEIFIGITSDKQWKAFGEAFTCPDLASDPRVQTNNDRILQ
                     RGWLIPELQKVFGSKSKEEIIRVCEKAGIPFAPIAHPEDLFEDPQLNEGGGLVEVPLP
                     QGGRTKLPRIPVRVGSHEFGLRSSAPSIGEGAREILKAAGISDQEVEQLKKEGIVAF"
     gene            5430..6902
                     /locus_tag="VEL68_18755"
     CDS             5430..6902
                     /locus_tag="VEL68_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ38072.1"
                     /translation="MITGKEFRERLAKLTPNVYMGGKPVDRFDPRLVGGMNVMAATYD
                     FAHDPEFQGVGVATSHLTGEKINRYCHIHQNTEDLINKQKMTRLYSQEVGGCIQRCMG
                     IDALNGISIVSHEADHQYGTRYYRNFVEFMKKVQKEDLSLNCAQTDVKGNRPWRPHQQ
                     KDPDLYLRVVEKKSDGIIVNGAKAHNSCSVYVDEIVAIPTRSLTPEEKDWAVAFSIPS
                     DWEGVTIINASYSPGKRKRLDCPYVNFGASHSITVFDHVFVPWERVFLCGETELAGKL
                     ALLFALYHRHSYTGCKAALSEIFTGATALAAEYNGIDKAQHVRHKLAEMIQIVDLIYS
                     SGIAAAVHSVKTPSGTYVPDSNFCNAGRMLAGENIYDEYESLAAIAGGLVATLPSEDD
                     FYNEKTGPYLEKYIMRDPNISAEHQHRAFRLFQDMMASAWGGHKLVDALHGGGSPVIE
                     KVAIYRDHNIERSKNIAKKLAGIPYDGSTKKLERKFPWLT"
     gene            complement(7513..7935)
                     /locus_tag="VEL68_18760"
     CDS             complement(7513..7935)
                     /locus_tag="VEL68_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38073.1"
                     /translation="MSVVFPVPTSPVMVIMPFFSLIPDLKEDRASPWLMPVKKKLGSG
                     VSLNGSSEKPKNSLYMGFPGPPRPDGNISGHPANIIRPLFPGILLYFLFPTMNGWPKN
                     IKYTNSLRGIFGTLEEFKNRILFSTLSPSKPLPERIFK"
     gene            complement(8108..8245)
                     /locus_tag="VEL68_18765"
     CDS             complement(8108..8245)
                     /locus_tag="VEL68_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38074.1"
                     /translation="MPDEKSQEEVCPRTLLLEKPVQVLQYFAAQVLSFINDNYHILMV
                     G"
     gene            8420..9643
                     /locus_tag="VEL68_18770"
     CDS             8420..9643
                     /locus_tag="VEL68_18770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ38075.1"
                     /translation="MICEFAKGIPRLVNKVCDQALFAGYSSSIQRIDAMIAKAVIREE
                     ENTTQEEPLEERETTVEKDSFGMINYSMPQEPVRFLEEDFLGLRGESMKKKDTMGVDR
                     DRKGSEPQPTSEPKEKAYWLYSGSFLGLYKSLKEKYIDLEVAKNQLQKERTELAAQIA
                     KKESELKEVQESLKVEGEKRRHAEEILGPMEERVRFLSSDFLDLQEKDKKFIARDLLD
                     VITSLILSLKGGAEDILHIMKGESNAALFNFEQILHNLQNGIQKTEEILAWLYPPTLD
                     ESGILSTIDWYCRKFQKYHTQFQISQEIDIQEDEISAPLKKQVFRILQETLDILAEYG
                     RGDRIGVSVIKKDEKVHLTVEENGQGFDLHKLFSNSKGIRLTRIKQRTELSGGSFSIE
                     SAEAVGTVIRSSWSI"
BASE COUNT         2403 a         2500 c         2579 g         2203 t
ORIGIN      
        1 aagagttcgg aaaccgcctg tcattctggg gagggatcga cacccatcgg gtcttgccat
       61 tcgggacccc gggcgaagta cgccgagaag tcagaaaaat gatcgattgc ctggggaagg
      121 gcggaggata ccttttagcg agcgtccaca acatccaggc cgaggttccc cccgagaaca
      181 tcgcggccat gttcgacgaa gcaagagaat acgggagata ccagcattag gttaaaggtt
      241 tgaggcccaa agccgggggc ggccggagag cggaaaatca gcccaggatc ctggagctgg
      301 cactccgaaa gttgttttta ttccgccgcc agcttcaccc tcttcccctc gatctccagg
      361 gtcgttttgc agtaggtgct tttccggata atcttcccat ctttgaaatg catcacatcc
      421 aaaccccttc tcttctcccg ttttccctgg tatcctttct caatcgacgg ccagtccagg
      481 gtccattcgt agaggacttt ttgggtttcc ccgtccacga aggtgtcttc accgataaaa
      541 cggaaattgc cgtggttttt gaaccaccct tcccaggctt tgcgcagcgc ttcctttcct
      601 tcggccctgc cgccggtcca gttttcaaaa acaatattct catggaagag cgccatgacc
      661 ccatcgaggt catggtcatc ccaggcccga ttccactggg ccagggcttt caggatctcg
      721 tttctcgata gcatggaaag agcctccttc ctcctgtcaa ggtgacatga cttgcgaatt
      781 tcattataat caaaaaagag aggttatgaa aggcccgcaa cccggcccaa ggagacatca
      841 tggcttcttc ccaaaaaatc ctgacgaacc tgaagaaact ggtcgagcaa tctcctgccg
      901 accagaccga agccctgctg atgaccgagg actcatccct tacgcgcttc acccctgcat
      961 cggttcatca gcacgtggcc gagcgcaatc agaccgtgat tctgagggtc gttttgggaa
     1021 aaaaggtcgc tgtggtgacc acgaatcttc tttcgccttc ttctttaaga accgcgctgg
     1081 ataaagcggt gtccttggcc cggtccttac ccgcgaatcc ggactttcga tctcttcctc
     1141 aggccaggcc gatccccgaa gtcaatacct tctttgagag catccgccga ctcactcccg
     1201 ggaagaaaat aaaggccatc caggagatca tgggcctggt gaaagaaaaa ggggcctccg
     1261 tttcaggagc tttctctcat gggaccgtgg aggtagccgt ccttaactcc ctgggggttg
     1321 aggcctttca gcaatactcc gacttctctc tccatttgat cgctcaaaat aaccatagct
     1381 cggggtatgc gagctttgtc tcccgggacc ctgatagact ggagatagaa tcccttgccc
     1441 gatctgcggt ctccaaaata tccatggaaa gacccgtcga aatttcgccg ggggagtacg
     1501 aagtgatcct ggaacccgat gcagcccatg aatttttgtc ctttatggga ttcctggggt
     1561 ttcacgccct ggcggtccag gaaggacgtt catttctggg caaccatttt ggagaaaagc
     1621 tcctggatga gagagtcacc atctatgacg acggcctaga tcctcacggg cttcaggtcc
     1681 ccttcgattt cgaaggggtg cccaagcaga aggtgttgtt tttcgacaag ggggtggccc
     1741 ggaacgtcac ctatgattct ttaaccgcgg gccgtgagaa gaaggaatcc accggacatg
     1801 ccctgacgcc gcccaacacg gaaggtccca ttccggtgaa tctttttatg gaagggggcc
     1861 attccagcct cgaaggaatg atccgaagca tccgaagggg aatttacgtg acccgttttc
     1921 attacaccaa cgtggtggaa cccatgaaag cagtgattac cggcatgacc cgggatggaa
     1981 ccttctggat cgaggacggg gaggtccgca atcccgtcaa gaacctgcgt ttcaccgaat
     2041 ccattttgaa agccctctcg caggtaaaag ccatttcccg cgaaaggaaa atctgcacct
     2101 ccggggcggt gtacggccgg aggttcgtga ccggaaccgt cgctcctgcg ctgcaaatcg
     2161 atgggttcta tttctccgga gtatcttcct tgtaagagca aggacgaaaa cttatttctc
     2221 tcgcagagcc cgcagggatc ggaaagagat attttttggt ttttcatttt gggcattttc
     2281 aaaggagttt tcgatgaagg gtctcatcta ccacctcctc gacatggcaa aggtgaggaa
     2341 ggcgacctat gccgacatcc ggatcgttca tcggcagatg gaggagattt cggtcaaaaa
     2401 cgggaaagtc gatgccgtga accagaatga agatcaaggg ttcggggtcc gggttctgtt
     2461 ccagggggtc tggggttttg ccgccagctc aataatcaac aaggcggaga tggaaaaaac
     2521 cctggcccgg gcctggagga tcgctcaggt aagttcgagg gttaaagaaa aggacgttct
     2581 gttttcccgg caggcagcga tcatggaaaa ttatcggacc cccgcgtcca tcgacccctt
     2641 ttccatctcc ccggaccgga ttatcgacct cctccttgaa tcggagaaaa tcctccgccg
     2701 gaatcctaag gtgaaggtct ccgaagcctt catgggagct tacaagactg aaaaaacctt
     2761 cgcctcgacc gaggggtctt tcatagagca ggagattgtg gagtgcgggg ctggaatctc
     2821 ggccacagcc atcgaaggat cggaagtgca ggtccgttcc tatcccaact cctttcgcgg
     2881 gaactttgcc acccgcggat atgaatggat cgagggattg aagataaagg atcatgccga
     2941 gaggattgcc gaagaggcgg tgcaactcct ctccgctaaa ccctgccctt ccgggaccac
     3001 gaccctcatc ctggaggggt cccagcttgc ccttcaggtc catgaatcca tcggccaccc
     3061 catagagctg gaccgaatct tgggaaccga ggccagctat gcagggacaa gcttcttgag
     3121 tctggacaaa gtcggtaaat ttccgtacgc atccgaggta gtgaacgtta ccgccgatgc
     3181 caccattccg ggcggattgg gaaccttcgg ttttgacgac gaaggaatca gggcccaaag
     3241 agtgcccatc ctctcccatg gggtgttgtc caacgtcctt acctcccggg aaaccgcccc
     3301 cctcctgaag aaagaaagca atggcaccat gcgcgccgac ggatggaacc ggattccact
     3361 catccggatg accaacatca acctggagcc cggcgagtgg acgctggagg ggatgatcgc
     3421 cgacacggat gaaggtctgt tcctgagcac caaccgcagc tggtccatcg atgataagcg
     3481 gatcaatttt caattcggca ctgagattgg atgggagatc aagaagggaa agctgggaga
     3541 tatggttaag aaccccacct acacggggat cactccccaa ttttggaact cctgcgacgc
     3601 cgtggccaac cgggactact ggctgctctg gggaacgccc aactgcggca aaggagagcc
     3661 cggccaggta gctcacgtgg gccatgggac cgcaccggcc aggttcagga acgttcgggt
     3721 gggtctaatg aaataagttt ttccgccact tgctggggaa tcagggggca tcttcccggg
     3781 cggcccaccc gactcatttt tgcttgacaa gatattaatt tctgatattc atggacaaaa
     3841 agggatactg attttccctc tttctcccta aaaactctga aggatatcgg ggtgttttcc
     3901 acctgcgaac gccttggggc gctggagaaa aggaaatctt ctgtttctga tccggtaata
     3961 actaatgacg gcgggagtga gcgatgaaac taccattgga tggagtcagg gtcctggaga
     4021 tgggacacac ggtgatgggc ccgacctgcg gactggttct ggcagatatg ggcgccgagg
     4081 tgatcaagat cgaacgggcc cccaagggag acgatacccg aagactcata ggctttgggc
     4141 agggtttctt tctctatttc aaccgaaata agaagagtct cgccgtggac ttgaaatcgg
     4201 agaaaggagc ggggctggtc aagaggcttg caacttcctc ggatgtattc atcgaaaatt
     4261 tcgggcccgg ggcggtggac cggctgggac ttgggtatga agacttgtcc aaggtgaacc
     4321 ctcgcctgat ctattgttcg ctgaaaggat ttctgcccgg cccttacgag caccgcccgg
     4381 ccctagacga agtggtccaa atgatgggcg ggctcgccta catgactggg ccccttggcc
     4441 gtcccctccg cgcggggtct tcggtcgtgg atatcatggg aggaagctac ggggtcatcg
     4501 ggatcttaac cgctctctat gagcgggaga agaccggcag gggccagttt gtgatcgctt
     4561 ccctgtatga atccaccgct ttcctgatgg gccagcacat ggcgctttcg gccgtctccg
     4621 ggaaaccggt cccgcccatg cccagtcgtg tctccacctg gggcatctat gacatcttca
     4681 agacccaaga cggggagatt ttcattggga tcaccagcga caaacagtgg aaagctttcg
     4741 gtgaggcctt cacctgtcct gatctggcca gtgaccccag ggtccagacg aacaacgacc
     4801 gaattctcca gcgcggctgg ctgattccgg aactacaaaa ggttttcgga agtaaatcca
     4861 aggaagaaat catccgggtc tgcgagaagg cgggaatccc ctttgccccc atcgcccacc
     4921 ccgaagacct cttcgaagat ccccagttga acgagggcgg aggcctcgtg gaggttcccc
     4981 tgccccaggg gggccgaacc aaacttcctc ggattccagt acgagtcggt tcccacgaat
     5041 tcgggctgcg ctccagcgct ccctccatcg gagagggggc ccgggagatc ctgaaggccg
     5101 ccggcatttc cgatcaagag gtcgagcaac tgaaaaaaga aggcatcgtc gctttttgaa
     5161 aacaatctcg ggcaagattc tctttcgccc tcgatgatct ggaagcggct attaccattc
     5221 gtcatgccca cgggggcagg tattcagaga gattacctta ttgacaaacc actcctgtga
     5281 ggccactctg gaagacactg gattgcatct tcctgatgtc acggggagag gttctgcggg
     5341 caggatggct ttcctatctc tttgccaaac ggtctctttg gaaaaaaggc gaaggagggg
     5401 tagggtttca gcttgaaagg aggcaggaaa tgatcacggg aaaggaattc cgggagagac
     5461 ttgccaaact cacacccaac gtctacatgg gaggaaagcc ggtggaccgg ttcgatcccc
     5521 ggcttgtggg ggggatgaat gtcatggctg cgacctacga cttcgcccat gacccggagt
     5581 ttcaaggagt tggtgtggcc acatcgcacc tgaccggcga gaaaatcaac cggtattgcc
     5641 acatccacca gaatacggaa gacctgatca acaagcagaa gatgacccgc ctgtacagcc
     5701 aggaggtggg gggctgcatc cagcgctgca tgggcatcga cgcgctcaac ggcatctcca
     5761 tcgtttccca cgaggcggac catcaatatg gcacccgcta ttatcggaat ttcgtcgagt
     5821 tcatgaaaaa ggtgcagaag gaagaccttt ccctcaactg cgcccagacc gatgtcaagg
     5881 gcaaccgccc ctggaggccg caccaacaga aagaccccga cctttacctg cgggtggtgg
     5941 aaaagaagtc ggacgggatc atcgtgaacg gggctaaggc ccacaattca tgctccgtgt
     6001 acgttgacga aatcgtggcc attcccaccc ggtccctgac tcccgaagaa aaagactggg
     6061 ccgtggcttt ttccatcccc tccgactggg agggagtcac catcatcaat gcctcctact
     6121 ctccgggtaa gcgaaagaga ctcgactgcc cctacgtgaa ttttggggcc agccattcca
     6181 ttactgtctt cgatcatgtt ttcgttccct gggaacgggt tttcctttgc ggagagaccg
     6241 agctggccgg gaagctggcc ctgctcttcg ccctgtacca ccggcacagt tataccggct
     6301 gcaaggccgc cctttcagag attttcactg gggcgacggc cttggcggcg gagtataacg
     6361 ggatcgacaa agcccagcac gtgcggcaca agctggcgga gatgatccag atcgtggacc
     6421 tcatctattc ttcagggatt gccgccgccg tccattcggt caagacccct tctggaacct
     6481 acgttcccga ttcgaacttc tgcaacgcgg ggcggatgct ggccggggaa aatatctacg
     6541 acgagtatga atccctggcg gccattgccg gcgggctggt ggccaccctt ccttccgaag
     6601 acgatttcta caatgagaag accggtccct acctggaaaa gtacatcatg cgtgacccga
     6661 atatctcggc cgagcaccag catcgggcct tccgcctttt ccaggacatg atggcttcgg
     6721 cctggggcgg gcataagctc gtcgacgccc ttcacggggg cgggtcgccg gtcatcgaaa
     6781 aagtggccat ttatcgggac cacaacatcg agcggtcgaa gaacatcgcc aaaaagctcg
     6841 cgggaatccc ctacgatggc tccacgaaga aattggagcg caaatttccc tggctgactt
     6901 gaatgaagct cataggatag agagcccatc tgggatgaac ctattcaaag gagagatcag
     6961 ggtaaaggac atctaagcgg tcctgtcttt tgacgaccac cccgtggtaa tcaagcctac
     7021 cggaaatgga tcgccgaagc cctggagcat ggagtagctg gcattaaggc ggagaaaaga
     7081 tgatttccgc cccctactat tcagcctgga cttttctttc gttccccctg tccgggagac
     7141 catccggtac tgtttttttt aatagaccca cgcccaaacg gaaatggtcc tagccgaagc
     7201 agatattttt ttccaacctt gggaaagcgc cacttactcg atttttacga aaattctttt
     7261 gataaatttg tgtaaagaat taaaaaaatt aaataaatcg taatttttta caatgccatg
     7321 caagtatgca aataccctta ttttcattgt gcattttcca gagaagaagc cattattcca
     7381 aaaatcgtta attctattta cgtatttctg gcccaacgac ggaataattt ttttataacc
     7441 aattaatatt attattattt tataaatttc ttgaaaggaa tgagaattgc tgagaaaatt
     7501 caacagcacg ttctacttga atattctttc cggtagtggt ttggagggcg agagggtact
     7561 aaatagaatt ctattcttaa attcttccag ggtcccaaaa attcccctta aggaattagt
     7621 atatttgata ttttttggcc agccgttcat ggtcggaaat aggaagtaaa gtaggatccc
     7681 tggaaacagc ggccggatga tattggccgg atgaccggag atgttaccat cgggcctggg
     7741 aggtcctgga aatcccatgt acaatgaatt ttttggtttc tcggaagaac catttaggct
     7801 tactcctgat cccagtttct ttttcacggg catgagccat ggggaggctc tgtcctcttt
     7861 gaggtccggg atcaaggaaa agaaaggcat tattaccatt acgggcgatg tgggaacggg
     7921 gaagaccacg ctcatctacg ccctgttgaa agacctgagc gacaatataa aaaccgcctt
     7981 tatctctaat cctcggctgc cctttaaaca actgttgaaa gccgttcttt gggatttgaa
     8041 ggtgccggtc atagggaaca gcacctttag tcttttgcac aaattcgacc tctatctcca
     8101 ggagagatca gccgaccatg agaatgtggt aattatcatt gatgaagctc agaacttgag
     8161 cggcaaagta ttggaggacc tggacaggct tttccagaag gagagtcctg gggcaaacct
     8221 cctcctgact cttctcgtcg ggcaactgga gctggaggct aacctggatt ctcaggaact
     8281 gcggcagctc aagcagagaa tcaccctcca gcgaaggcta agcccattaa attgggaaga
     8341 atccaaggcc tacatcgacc accgcctgaa aattgtcggg agcagcattt ccaaggtctt
     8401 tactccggag gcggttgatt tgatctgtga atttgccaag ggaattcccc ggttggtcaa
     8461 taaggtttgc gaccaggccc tttttgcggg gtacagttcc tccatccaaa gaatcgatgc
     8521 gatgattgcc aaagcggtca tccgtgagga agagaatact acccaagaag aacctcttga
     8581 ggaaagagaa acaacggtcg agaaagactc tttcgggatg ataaactatt ctatgcctca
     8641 ggagccagtt aggtttttgg aggaggattt tttagggtta cggggggaga gcatgaagaa
     8701 gaaggatacc atgggtgtgg atcgagaccg aaaaggatca gagccccagc ctacctcgga
     8761 gccaaaggag aaagcctact ggttgtatag cggaagtttt ctggggctct acaaatctct
     8821 caaagagaaa tatatcgacc tcgaagttgc taaaaaccaa ttgcagaaag agcgtacgga
     8881 actggcagcc caaatcgcaa aaaaagaatc tgagttaaag gaagtccagg aatctttaaa
     8941 ggttgaagga gaaaaaagaa ggcatgcaga agaaattctg gggcccatgg aggaacgggt
     9001 tcggttttta tcctccgact ttcttgatct tcaggaaaaa gacaagaagt tcattgcccg
     9061 ggacctcctc gacgttatta cgagcctaat cctttcccta aagggcggtg cagaagatat
     9121 tctccatatt atgaagggag aatcgaatgc cgcgttattc aacttcgagc aaatccttca
     9181 caacctccaa aatgggattc aaaagacaga ggaaatcctg gcgtggctat atccccccac
     9241 cttggatgaa tccggaatat tgtccacgat tgattggtat tgccgtaaat tccagaaata
     9301 tcatacccaa tttcaaattt cacaagaaat tgatatccaa gaagatgaaa tcagtgctcc
     9361 tttaaaaaaa caggtattta gaatcttgca ggaaactctg gatattcttg ccgagtatgg
     9421 tcgaggcgac cggatcggcg tttcagtaat aaaaaaggat gaaaaggtcc atttaacggt
     9481 agaagaaaat gggcagggct tcgaccttca taagctgttt tccaattcaa aaggaatccg
     9541 gctcacgcgc atcaaacaac gcaccgagct ctctggcggg tcattttcca ttgaatctgc
     9601 ggaagcagtc gggactgtta ttcggtcatc ctggtcgata tgactcctta aacggatggg
     9661 aattttcctt gcattcattt ttttt
//
LOCUS       DASPRR010000511         4456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_271597, whole genome shotgun sequence.
ACCESSION   DASPRR010000511 DASPRR010000000
VERSION     DASPRR010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271597"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..182)
                     /locus_tag="VEL68_18775"
     CDS             complement(42..182)
                     /locus_tag="VEL68_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38076.1"
                     /translation="MEGKAPAEPDDPNSSTLNPNKHEKLGVGKLAGWWAISLDTPLPF
                     FV"
     gene            561..2039
                     /locus_tag="VEL68_18780"
     CDS             561..2039
                     /locus_tag="VEL68_18780"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HXZ38077.1"
                     /translation="MKSWKWIGLWVGMGVLILVGFMVFRAVNVRSLQPAGQPQPTYSV
                     DSNLVCQHLSEAVRIPTINTGEGPNSPDQRDLLLFHQFLKNTFPLFHHFLKWEAIGGK
                     SLLYKWPGQSPDRPPLLFAAHMDVVPIDPKSEGQWKFHPFSGTIAEGFIWGRGTLDDK
                     HSLLALLEAAELLLKKGVRPAQTIYFAFGHDEESGGSYGARQIAETLKGRGVRLEAVI
                     DEGMVILEGGIPGINSPVALIGIAEKGSVSFNLTVQVPGGHSSSPAPENAVGILARAV
                     TRIGDNPFPAEISAPVRLMFDYLAPEARYPEKLVFTNLWFFEPLLKRMLLRRPESAAM
                     LRTTSVATMFQAGVKENVIPEQASAVVNLRLAPGDSVKAVQERLSRIIHDSRVQILPR
                     GERLKGDPSKVSSPASRMFPILGRTARSLFPGTVVAPALMIAGTDTRHYETLTDNIFR
                     FIPMVLKPEDIRRTHGVNERLGVENYSQIIKFYASVMEEASR"
     gene            complement(2069..>4456)
                     /locus_tag="VEL68_18785"
     CDS             complement(2069..>4456)
                     /locus_tag="VEL68_18785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HXZ38078.1"
                     /translation="SYLYLPPSWTLVGILGVGMGLVVGFAAGKRRTGLLLSVAVFVLF
                     GFLFIGRILHPDFSPNHLIHYAGDAKFNIEGLIYRPPEPLEDRVRVHVRAEKIHQGGN
                     QFPVEGNLLLTVKDKQVDFRYGDRVRFISRIYVPRQATNPGSFDYRRFLAYQGIWITA
                     YANSSAEIVRTEEKKGNPFFHFVESGREKIRKFFDENAPPETAGIIKALVLGDRGDIS
                     KEVNERFIISGVNHILSISGLHVALVAAFFFGATRFILRFFPFLLLRLNLNKTSALLA
                     IIPVIFYTFIAGLGVAAVRSTIMVLSFLLALLLDREKDLYDALFVAAFFILIFNPAAL
                     FDISFQLSFLSVLALLYFIPRFKEFFSFLKTWPYKSWLEEQPPWRRIFVFYLAGTLLT
                     STAAILGTGPLVGYYFNRISLVGLLSNLILVPLMGFANTLLSLLAAVMVFLCQPLAKI
                     LTELNSFILEISLALVDLFSRVPMASHRVVTPTIPEVLLIYGILVLAANLKRWKRSLH
                     ALILLAAVYGGIQIHGYVSIHHSQELKVTFLDVGQGDAAVIQLPRGKVMVIDGGGTPD
                     GSFDPGERIVAPYLWKEKRKAVDYMVNSHSHPDHLQGLLFLLENFEVKKVWDNGEREA
                     ESPLVQKMLEMAWDRVEARGQGSSVQEVEEVKIEFLHPPLEKAKRFPFWGNDASLVLR
                     LTYGEVSFLFCGDVESLGEQEILKAGGNLKSTVLKVPHHGSKSSSTPDFLEAVRPRFA
                     VFTVRGGARPQLPNLGVLGRYEAMGVKVLRSDRDGAITFTTDGKDLRVRTFLGKD"
BASE COUNT         1027 a         1258 c         1245 g          926 t
ORIGIN      
        1 caccgagccg attgataaaa actcgcttta tgatggtttc atcatacaaa aaaaggcagt
       61 ggtgtgtcaa ggctgattgc ccaccatccc gctagcttgc ccacccccag tttttcatgt
      121 ttgttggggt tcagggtgga ggaattcgga tcatcaggct cggcgggtgc ttttccttcc
      181 atgaaactcc tccccatgtt ccgcaggcag aggcctgaga ggagatccct ccaagcagaa
      241 aagagagcca aagcatgttt ccgttccaat cccccgtagg acaggcccga tccaatggaa
      301 gaaccaccgc ctcaggatga tgatccaaat ttctccgccc tgaaccccag gagatccccc
      361 ctcctttaca gaggtcttat tttcgaagga ctcttcttta agaactgggg gtagacaatg
      421 atcaaaccca cttgacacaa tttccgcgct tgatatcatc cgactgacgc cagactgcct
      481 cttctccaaa acggttcttg gtcttcgggg ctgtgaacaa cttgccgcgc aagattctct
      541 tatagcaagg ggacaaagag atgaaatcgt ggaaatggat cgggttgtgg gtcggtatgg
      601 gagttctaat tctcgtcggt tttatggttt ttcgcgccgt aaatgtccgc tcgctccagc
      661 cggccggaca accgcaaccc acttattctg tcgattccaa cttggtctgc cagcatctct
      721 ccgaggcggt ccgcatcccg accatcaata ccggagaggg tcccaattca cctgaccagc
      781 gggatctgct tctctttcac caatttttga aaaacacgtt ccctcttttc caccatttcc
      841 tcaagtggga agcgatcgga ggcaagagtc tcctgtacaa atggccgggg cagagtccgg
      901 accggcctcc ccttctcttt gcggcgcata tggacgtagt cccgatcgat ccaaaaagtg
      961 agggacagtg gaagttccat ccgttctcgg gaacgatcgc ggaagggttt atctggggaa
     1021 gggggactct ggatgacaaa cattccctcc ttgcccttct ggaagccgca gagcttctcc
     1081 tgaaaaaggg ggttcggccg gcgcagacaa tctactttgc ttttggccac gatgaggaat
     1141 caggcgggtc ttacggtgca cggcagatcg ccgagacgct gaagggccgg ggagtccggc
     1201 tcgaagcggt catcgatgaa ggcatggtca ttctggaagg cggaatcccg ggcatcaaca
     1261 gccccgtggc cctcatagga attgcggaga aagggtccgt cagtttcaat ctcaccgtcc
     1321 aggtccccgg cgggcactct tcgagcccgg cccctgagaa tgcggtcggt attttggccc
     1381 gggcggttac tcgtatcggg gataaccctt tccccgcgga aatatctgcc ccggttcggc
     1441 tgatgtttga ttaccttgcc cccgaggccc gctatcccga aaagctcgtc ttcaccaacc
     1501 tatggttttt tgaacccctc ctgaagcgaa tgcttttgcg ccgcccggag agcgccgcaa
     1561 tgctgagaac gacttcggta gccaccatgt ttcaggccgg ggtgaaggag aatgtgatcc
     1621 cggagcaggc ctcagccgtg gtgaaccttc ggcttgcccc gggggactcg gtgaaggcag
     1681 tccaggagcg actctcccgg atcatccacg actcccgcgt ccaaatcctt ccacgggggg
     1741 aaagattgaa gggtgacccc agcaaggtct caagcccggc ttcccggatg tttccgatcc
     1801 tcgggcgcac cgctcgctcc ctcttcccgg gaacggtggt tgcgccggcg ctgatgatcg
     1861 ccgggacaga tactcgccac tatgaaactc tcactgataa catcttccga ttcatcccca
     1921 tggtcctgaa accggaggat atccggagaa cgcacggggt gaacgaacgg cttggggtcg
     1981 agaactatag ccagatcatc aagttttacg ccagcgtgat ggaagaggcg agccggtaag
     2041 agcccttacc cggcggagga tttcgccttt aatctttgcc caggaaggtc cgaactcgca
     2101 ggtccttccc atccgtagta aaggtgatcg ctccgtcacg gtccgagcgc aggaccttta
     2161 cccccatcgc ttcatacctt ccaaggaccc ccagattcgg aagctgggga cgcgctccgc
     2221 cccggaccgt gaagaccgcg aaccggggac gaacagcttc caggaagtcc ggagtgcttg
     2281 aactcttgct gccgtggtgg gggactttta gcaccgtgct tttcaggttc cccccggcct
     2341 tcagaatttc ctgctccccg agggattcca catccccgca gaagagaaaa ctcacctctc
     2401 cgtaggtcaa ccgcaggacc agggaggcgt cgtttcccca gaagggaaat cgtttggcct
     2461 tctccaaggg gggatggagg aattcaatct tcacttcctc cacctcttgc accgaactcc
     2521 cttgccccct ggcttccact cggtcccagg ccatctccaa cattttctga acgagaggag
     2581 attcagcctc ccgctcccca ttgtcccaca ccttcttgac ctcgaaattt tccagaagga
     2641 agaggagtcc ctggaggtga tccgggtggg aatgggaatt gaccatataa tcgacggctt
     2701 tccgtttctc tttccaaaga tacggggcga cgattcgctc tcccgggtca aagctgccat
     2761 cgggggttcc ccccccgtcg atgaccatca cctttccccg gggcagttgg atcaccgcgg
     2821 cgtctccctg gcccacatcc aggaaggtaa ctttgagctc ctggctgtga tggatggaga
     2881 catacccgtg gatttgaatt cctccgtata ccgccgccag caggatcaag gcatgaaggg
     2941 agcgcttcca gcgcttcagg ttggcggcca ggacgaggat tccatagatc agcagcactt
     3001 ccggaatggt cggagtgacg actctatggg aggccatggg gacccggctg aaaaggtcga
     3061 ccagggccag ggaaatttcc agaataaaag agttcaactc ggtcaggatt ttggccaggg
     3121 gctggcagag aaaaaccata accgcagcca gaaggctcag gagggtgttg gcaaacccca
     3181 tcaggggaac gagaatcagg ttggagagga gacccacgag agaaatccgg ttgaaataat
     3241 atcccaccag cggccccgtg cccaatatgg cggccgtcga ggtgagaagg gtgccggcga
     3301 ggtagaatac gaatattctt ctccagggag gttgctcttc cagccaactc ttgtaaggcc
     3361 aggtctttaa gaaactgaaa aattccttga aacgagggat gaaataaagg agagccaata
     3421 ccgagagaaa cgagagctgg aaggaaatgt caaagagggc cgccgggttg aagatgagga
     3481 tgaagaaggc cgcaacgaag agggcgtcgt agaggtcctt ctcccggtcc aggagcaggg
     3541 ccaggaggaa agagaggacc atgatcgtag accgcaccgc tgcgactccc agcccggcga
     3601 tgaaggtgta gaagatcacc ggaattatgg ccaacagggc cgaggttttg ttcagattca
     3661 ggcgaaggag cagaaagggg aagaatcgga gaatgaaccg ggtggctccg aaaaagaagg
     3721 ccgccaccaa agccacgtga aggcccgaga tggaaaggat gtggttcacc ccggaaatga
     3781 tgaaacgctc attgacctcc ttggatatgt ccccccgatc ccccagcacc aaagctttga
     3841 tgatgccagc ggtttccggg ggagcgtttt catcgaagaa tttccggatt ttctcccgtc
     3901 cactttcaac aaaatggaag aaggggtttc cttttttctc ttccgtgcgc acgatttcgg
     3961 cagagctgtt ggcataagcc gtaatccaga tcccctgata ggccaggaac ctccggtagt
     4021 cgaacgaccc cgggttggtg gcctgcctgg gaacataaat ccgggagatg aaccgaaccc
     4081 ggtctccata acgaaaatcg acctgtttgt ccttgaccgt caggaggaga ttcccctcca
     4141 ccgggaactg gttaccccct tgatggattt tttccgctcg tacatgcacc cgcactctat
     4201 cttccagggg ttcagggggg cggtagatca ggccctcgat gttgaatttc gcgtctcccg
     4261 cgtagtggat gagatggttc ggggaaaagt cgggatggag aatacggccg atgaacagga
     4321 atccgaagag gacaaaaacc gcgaccgaaa ggagcagccc cgttcttctc ttccccgcgg
     4381 caaaaccaac caccaggccc attcccacgc ccaggatccc aaccaaggtc cacgaaggag
     4441 ggagataaag gtagct
//
LOCUS       DASPRR010000512         6191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_440639, whole genome shotgun sequence.
ACCESSION   DASPRR010000512 DASPRR010000000
VERSION     DASPRR010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440639"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2088)
                     /locus_tag="VEL68_18790"
     CDS             complement(<1..2088)
                     /locus_tag="VEL68_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HXZ38079.1"
                     /translation="MAPDSPSLDSDRVRRGSDPSQDLFDSDALRINLEETAVDQVAVD
                     PKYKVLQETVSGYRGILKSLDTLLYELNHPYKNWEIILPELRGFALKYFSSYSRHPKG
                     AQAVSSMIEIFLDALLNSQREDLEAKAIDHLLSYLEKVLNEMNPANQESFLPTLQACF
                     ERLSSMPEKKFFLLASSHIPLKRIGQVLLRKVSDGSGLSSFNRLLAQSLRVAYGYWLK
                     EEDPQAWFVPGAGDLPRPWEKEPDILDGISHQRLREYQTRLDQLSVRDGSAETLQSLL
                     ALPGYLEIVRAYKEIPHRLGADSDGGGDPLTQGWKILSLFKIMEIPGLKDIREETLRE
                     INHTLVALIKKESPERVGEILIKTFSLLKSNVENYPQTALQCIQAIGAEVFNREYSPL
                     VETFLEQVLRFGFQYPGYEGVNSNWQILCNPCHVVNVRVWLDIISRNPKWCSTLLSAL
                     IINLQLGGTCIKDTDLFQKEITKLLNSDTEPVYNLVKQLTKALPVYFNEIGAEGQLRD
                     VTTDIDEIRNRKDILIHFLRKQSHVESSNLIEDFIREIFRFWRLKDKSGLRRFLPAEV
                     HHPLKTEGPFIDEVCGILRELFLQKGFQKEEDLLRLSESEVESILAELPGFSDPEKKR
                     VDLLIKVYRLINQKYHLGFQEMRYHLQQASRWGFEGLDELQKVLDRNDTLECLEAILT
                     YLEGLKEITTSAER"
     gene            complement(2106..3110)
                     /gene="gap"
                     /locus_tag="VEL68_18795"
     CDS             complement(2106..3110)
                     /gene="gap"
                     /locus_tag="VEL68_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXZ38080.1"
                     /translation="MAIKIGINGFGRIGRSVFRAALQGKEFSNAEIVAINDLTDNATL
                     SHLLKYDSIMGRLNAEVKVTEKGLVVDGKTVVITARKDPAQIPWRDLGVEYVIESTGL
                     FTDSGRARAHLDAGARKVIITAPAKNEDLTVVMGVNEPDYNPYQHHIVSNASCTTNCM
                     APIAKVILEKFGIRRGLMTTIHSYTNDQRILDFPHSDLRRARAAALSMIPTKTGAAQA
                     VALVIPELKGKFDGLAVRVPTPNVSLVDLVIELEQEATVQDVNQALKEAANRYLGYTE
                     EPLVSIDFQGDAHSSVVDGSSTKVTGTMAKVLSWYDNEWGYSNRVLDLILYMDSQKPV
                     "
     gene            3586..4770
                     /locus_tag="VEL68_18800"
     CDS             3586..4770
                     /locus_tag="VEL68_18800"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXZ38081.1"
                     /translation="MAFRSIDQVDIAGKRVFMRVDFNVPLDKNRNIGDDTRIQAVLPT
                     LKYALSAKAKIILASHLGRPKGKPEPEFSLAPVARRLGDLLKKEVPLAPDCIGPEVER
                     MVEALKPGDVLLLENLRFHKEEEKNDPGFAQSLAKLADVYVNDAFAVSHRAHASVEGM
                     TKFFKDKAAGFLMRDELHYLGKVMEDPARPLVAVIGGAKVSGKLEVLQNLAPRVNKLI
                     IGGGMAFTFLKAVGMNVGKSLVEENLVKTAQGILEEARKKGVKVFLPVDCVAAEKMDP
                     SAKAKVVPVDKIPGDSMGLDIGPETLNRFGEALKDARTILWNGPMGVFEMEPFSKGTL
                     GMIRHIISSGAMSVVGGGDTDTAVHKSGQADKFSYISTAGGAFLELLEGKKLPGVEAL
                     KN"
     gene            4922..5683
                     /gene="tpiA"
                     /locus_tag="VEL68_18805"
     CDS             4922..5683
                     /gene="tpiA"
                     /locus_tag="VEL68_18805"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXZ38082.1"
                     /translation="MPRIPLIAGNWKMFKTIPETLDFCQRLKGEVVALQGKQIEVAVA
                     PPFTALAAAAHALQGTSIAIAAQDVFWAESGAYTGEVAPRMLKDAGCRMAIVGHSERR
                     QYFHETDESVNKKTAALMKEGLTPIVCVGETLAERKERVTFRIIERQITQGLKGVSLK
                     DPLLLVIAYEPVWAIGTGQTATPQQAQEVQAYIRKLLGEVFSSSIAQGMRILYGGSVK
                     PENTAELMAMEDVDGALVGGASLQIDSFCQIIKRA"
     gene            5836..>6191
                     /gene="secG"
                     /locus_tag="VEL68_18810"
     CDS             5836..>6191
                     /gene="secG"
                     /locus_tag="VEL68_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXZ38083.1"
                     /translation="MGLALAIVHILVCLLLIMIVLLQTGKGADMGAAFGGGSSQTLFG
                     SAGPAGFFTKITTGVAIIFMVTSIGLAYMYSHRSGVSSVRETRPAKSAPVQPPTPVEK
                     GIPIQPAPAQPAPQPGT"
BASE COUNT         1342 a         1633 c         1825 g         1391 t
ORIGIN      
        1 ccgctccgca gaggtggtga tttccttcaa cccttccagg taggtcagga tcgcttccag
       61 gcattccagg gtgtcgttgc ggtccagaac cttctggagt tcgtccagcc cctcaaagcc
      121 ccaccgggag gcctgctgca ggtggtagcg catctcctga aaccccaggt gatatttttg
      181 gttgatcagg cgatagactt tgatcaaaag gtccacccgc ttcttttccg ggtccgagaa
      241 tccagggagc tcggccagga tcgactccac ctccgattcg ctcaggcgca ggaggtcttc
      301 ctccttctgg aagcccttct gcagaaaaag ttcccgcagg attccgcaca cttcgtcgat
      361 gaaagggccc tcggtcttca ggggatgatg gacttccgcg ggcaggaacc gacggagccc
      421 acttttgtcc ttcaaccgcc agaaacggaa aatttcccgg atgaaatctt caatcaggtt
      481 gctgctttcc acatgactct gcttcctcag gaaatggatg agaatgtctt tccggttgcg
      541 gatttcatcg atatcggtgg tcacatcccg cagctggccc tcggccccga tctcgttaaa
      601 atagaccggc agggctttgg tcagctgctt gaccaaattg tagaccggct cggtgtcgga
      661 attcagaagt ttggtgattt ctttttggaa aaggtcggta tccttgatac aggtcccgcc
      721 cagctgcaag ttgatgatca gggcggatag gagggtgctg caccatttgg ggttccggga
      781 aatgatgtcc agccaaaccc ggacgttgac cacgtggcag gggttgcaaa ggatctgcca
      841 gttggaattc accccctcgt accccgggta ctggaagcca aagcggagga cctgctccag
      901 gaaagtttcg accagcgggc tgtactcccg gttgaatacc tcggccccga tggcttggat
      961 acactggagg gcggtctggg gataattctc cacattcgat ttgaggaggg aaaaagtctt
     1021 gatcagaatc tctcccaccc gctcggggga ttccttcttg atcagggcca ccagggtatg
     1081 gttgatttcc ctcagggtct cctcgcggat gtccttgagc cccgggatct ccatgatctt
     1141 gaagagggat aagattttcc agccctgggt taaagggtcc ccgccgccgt ccgaatcggc
     1201 tcccaggcga tggggaatct ctttgtaagc ccggacgatc tccagataac ccggcagagc
     1261 cagaaggctc tgcagggttt ccgccgaccc atcccggacg gaaagctgat ccaaccgggt
     1321 ttggtattcc cggaggcgct ggtgggaaat tccgtctaag atgtccggtt ccttctccca
     1381 aggtcggggg aggtcgcccg ccccgggaac aaaccaggcc tgggggtctt cttccttcag
     1441 ccaataccca taggccaccc gcaggctctg ggccagaagc cggttgaagg aactcaggcc
     1501 ggacccatcg gaaactttac ggagcaggac ttgaccgatt cttttcaagg ggatatggct
     1561 ggaagcgaga aggaaaaact tcttctccgg catggaggag agcctctcaa agcaggcctg
     1621 gagcgtgggc aggaaactct cctggttcgc cgggttcatc tcgttgagga ctttttcgag
     1681 gtagcttagg aggtgatcaa tggccttggc ttccaagtcc tcccgctggg agttcagcag
     1741 ggcatccagg aaaatctcga tcatcgacga aacggcctgg gctcccttgg ggtggcggga
     1801 gtaggaagaa aaatacttca aggcgaatcc ccgaagctcc ggaaggatga tctcccaatt
     1861 cttgtaggga tgattgagct cgtagaggag agtgtcgagg gatttgagga tcccccggta
     1921 tccggacacg gtctcctgga gaactttgta cttggggtct acggcaacct ggtccacggc
     1981 cgtctcttcc aggttgatgc ggagggcgtc cgaatcgaag aggtcctggg acgggtcgct
     2041 gccccggcgc acccggtcag agtccaggga gggggaatca ggggccatgg aaaagactcc
     2101 gttctctaga caggcttctg tgaatccatg taaagaataa ggtccagtac ccggttggaa
     2161 taaccccatt cgttgtcata ccaagacagg actttggcca tggtgccggt gactttggtg
     2221 gaagagccgt cgaccaccga agaatgagcg tccccctgaa agtcgatgga gaccagaggc
     2281 tcctcggtgt atcccaggta gcggttggcg gcttctttca aagcttggtt cacgtcctga
     2341 acggtggcct cctgctccag ctcgatcacc agatcgacga gggagacgtt cggggtcggc
     2401 acccgtacgg ccagtccgtc gaacttcccc tttaactccg gaattaccag ggccaccgcc
     2461 tgggccgctc ccgtcttggt tggaatcatg gagagagccg cggcccgggc ccgccgcaga
     2521 tcgctgtggg gaaagtccag gatgcgctgg tcgttggtgt aggaatggat ggtggtcatc
     2581 agtccgcggc ggatgccgaa tttttccagg atgaccttgg cgatgggggc catgcagttg
     2641 gtcgtacagg aagcgttcga tacgatgtga tgctggtagg gattgtagtc gggctcgttc
     2701 actcccatga ccacggtcag gtcttcattc ttggccgggg cggtgatgat caccttgcgg
     2761 gctcccgcat ccaggtgggc gcgggctcgg ccggagtcgg tgaagaggcc ggtggactcg
     2821 atcacatact ccacgcccag gtctctccag ggaatttgag cggggtcctt gcgggcggtg
     2881 atgactacgg tttttccatc gactaccagg cctttttcgg ttaccttcac ttccgcgttc
     2941 aggcggccca tgatggaatc atattttaat aaatgggaca gggtagcatt atcggtcaga
     3001 tcgttgatgg ccacgatctc ggcgttggag aattcctttc cctgcagggc ggcgcggaaa
     3061 acacttcgcc cgatcctgcc aaagccatta attcctatct tgattgccat ggccgactcc
     3121 tttctgccgg gcaagattga acgggagaat ctcttcccct taaggatgaa aaattaattc
     3181 cttttactat aaatatcatt aacatgggcc aaagtcaaaa gctttaaacc cgcggtttcg
     3241 ttcggagtaa tgtaattcgg agttcggatc aaagatctct ctatcagctc ttcaacgcct
     3301 tcggtcggtt catgtatctc cggacttcga gctaaacaca gcatcctata tcggcagcag
     3361 gaatcagggc taaagccctg agttacaagc atccctgaag ctgtaactca gtcctttagg
     3421 gctgattgca cgatatgaag gcagatgatg ctgtattctt tatgccgctg ggtataaatg
     3481 accccgaact gtttatcatc aaagcattga ctttaggcaa tgaaaactcc tattatgatg
     3541 ctaagctccc tttgattcca tatctttggg gaaggaggat tgtttatggc cttcaggagc
     3601 atcgatcagg tcgatattgc cgggaaaaga gtattcatgc gggtggattt caacgtcccc
     3661 ctcgacaaga atcgaaacat cggcgatgac accaggatcc aggccgtcct tcccaccctg
     3721 aaatacgccc tttcggcgaa ggccaagatc atcctggctt cccacctggg aaggcccaag
     3781 ggaaagcctg agcctgagtt cagtcttgcc ccggtggccc gcaggctggg agatctcctc
     3841 aagaaagagg tccccctagc cccggattgc atcggcccgg aagtggaaag aatggtggaa
     3901 gccttgaagc caggagatgt cctcctcctg gagaacttgc ggttccacaa ggaggaggag
     3961 aagaatgacc cgggttttgc ccaaagcctg gccaagctgg ccgacgtata cgtgaatgat
     4021 gccttcgccg tctcccaccg cgcccacgct tcggtggagg gaatgaccaa gtttttcaaa
     4081 gacaaagccg ccgggtttct gatgagggat gagcttcact atctggggaa ggtcatggaa
     4141 gacccggccc ggcctttggt ggcggtgatc ggcggggcca aggtttcggg gaagttggaa
     4201 gttctccaga accttgctcc ccgggtgaat aagctgatca tcggaggggg aatggctttc
     4261 accttcctga aagctgtggg aatgaacgtg ggcaagtcat tggtggagga gaacttggtc
     4321 aaaaccgcgc agggcatttt ggaggaagcc aggaaaaaag gagtgaaggt ctttctcccg
     4381 gtggactgcg tggcagccga aaaaatggac ccctcagcca aggcgaaggt agtgccggtg
     4441 gacaaaattc ccggcgatag catgggtctg gacatcggtc ctgagactct gaaccgcttc
     4501 ggcgaagccc taaaggatgc aaggacgatc ctctggaacg ggcccatggg agtttttgaa
     4561 atggaaccct tcagcaaagg gaccctgggc atgatccgcc acatcataag ctccggggcc
     4621 atgagcgtag tggggggagg agatacggac acggccgtgc acaagtcggg gcaggcggat
     4681 aaattctcct atatctccac ggccgggggc gcctttctgg agttgctcga gggtaagaaa
     4741 ctccccggcg tggaagcttt aaaaaactaa tgttcaccac agagacagag agatcacgga
     4801 gagagaacca aattttgaat gaggcaataa acctaaaacc ctattttctg ttttggaccc
     4861 aaaaattctc tgtgtcctct gtgcctctat ggtgaaggct ttttgaaagg agttggaatt
     4921 tatgccccga attccgttga tcgccgggaa ctggaagatg ttcaagacca tcccggagac
     4981 cctggatttc tgccagcggc ttaaaggcga ggtcgttgcc ctccagggca aacagataga
     5041 agtggctgta gccccaccct ttaccgccct ggctgcggct gcccatgccc ttcagggcac
     5101 ttccatagcg atagctgcac aggatgtctt ctgggcggag agcggagcct ataccggaga
     5161 ggttgctccc cggatgctga aagatgcagg ctgccgtatg gccattgtcg gccactcgga
     5221 gcggaggcag tatttccatg aaaccgacga gtcggtcaac aagaagacgg ctgccctgat
     5281 gaaggaaggt ttgactccca ttgtttgtgt tggcgaaact ctggctgaac ggaaggaaag
     5341 ggtgactttc cggatcatcg agcgccagat cacgcagggg ttaaaggggg tgtcgctgaa
     5401 agaccccctt ctcctggtga ttgcctatga gccggtttgg gccatcggga ctgggcagac
     5461 ggcaactccc cagcaggccc aggaggtcca ggcttacatt cggaagcttc tgggagaggt
     5521 tttttcttcc tccatcgccc aggggatgcg aatcctctac ggggggagtg tgaagccgga
     5581 aaacaccgcc gaactcatgg ccatggagga cgtggatgga gccctggtcg ggggagcgag
     5641 cctacagata gattcctttt gccaaatcat aaagagggcg taaaccaaga aatccagtcg
     5701 ccttctggtc gcaccgctgc ttgggctggg ggacgcagtt tcccagcgtg agcctttaac
     5761 ctcctttttt atctggattt tttcccgggg gtgtgctata agaattttta cctcgggtgg
     5821 gaggcttttt cgattatggg cttggctctg gcaattgttc atatcctggt atgtttgctt
     5881 ttgatcatga tcgttcttct ccagaccggc aaaggggcgg acatgggggc ggccttcggc
     5941 ggagggtcca gccaaaccct tttcgggtct gcgggcccgg ccggcttttt caccaagatc
     6001 accaccggcg tagcgatcat tttcatggtc acttccatcg gtttggcata catgtacagc
     6061 caccggtcgg gcgtttcctc ggtgcgggag acaagacccg ccaagagcgc cccggttcaa
     6121 ccaccaactc cggtggaaaa gggtatcccc attcaacctg cgcctgctca gccggcgccc
     6181 cagcccggaa c
//
LOCUS       DASPRR010000513        20833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1416257, whole genome shotgun sequence.
ACCESSION   DASPRR010000513 DASPRR010000000
VERSION     DASPRR010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 20833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20833)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20833
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416257"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..545
                     /locus_tag="VEL68_18815"
     CDS             3..545
                     /locus_tag="VEL68_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HXZ38084.1"
                     /translation="MVRKGWKERAKKVRLLLLDVDGVMTDGRLAFDGEGKELKFFYAR
                     DGIGIKLLQEAGIRVGILSGRRAKVVALRAKELGISLVRQKTRDKAQALEEILEAENL
                     RPEQVCYVGDDLVDLSVLRRVGLPVAVADAVPEVKFMARLVTRNPGGRGAVREVCEKI
                     LKIQGKWEKGMRKFRSSPAG"
     gene            680..1273
                     /gene="lptC"
                     /locus_tag="VEL68_18820"
     CDS             680..1273
                     /gene="lptC"
                     /locus_tag="VEL68_18820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HXZ38085.1"
                     /translation="MRRSITLRSLIGVIILGSLGLVGVLVWQALTLRSTEKISPSKPD
                     ASADLRLDRVHYTETREGVKEWEMEATSAVYRKEGDTMVLEKIRATFYGKNQEIYHLV
                     GEKGKFNTQTKVIEVYDGVTIDSDDGYHMRTRSLKYQADQKELHTSDPVELEGPSMLV
                     EGVGMVVDLNRERVKILRDVTTTLPSLDWKKSVRSAR"
     gene            1275..1823
                     /gene="lptA"
                     /locus_tag="VEL68_18825"
     CDS             1275..1823
                     /gene="lptA"
                     /locus_tag="VEL68_18825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HXZ38086.1"
                     /translation="MKSKGIVWAMGVFFLLLAPLLPGWAAEKKPALLGKATQEGSAQP
                     LRITSQQLEADNKSQVITFTGNIIARQGDMVIYADVARVYYEKREEGNEVREIVAEGN
                     VKIHQGDRVATGERAQFLNGEQKIILTGQPRVWQGKDMVSGEKITVLLEEDKSLVESG
                     PDRRVEVILYPKGEAGKAKGKP"
     gene            1820..2551
                     /gene="lptB"
                     /locus_tag="VEL68_18830"
     CDS             1820..2551
                     /gene="lptB"
                     /locus_tag="VEL68_18830"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXZ38087.1"
                     /translation="MSSVQTLLARGLKKQYNSKWAVDSIDLEFRSGEVIGLLGPNGAG
                     KTTTFYIIVGLIRPDGGEIFLSGEEITHDPMYLRARKGVTYLPQEPSVFRKLTVEENL
                     MAILESLDLTQEERQKRLKTLLEDLRISGLAKRKAYSLSGGERRRVEISRSLVTSPRF
                     ILFDEPFAGIDPLAVVDIQNIIVQLKSRGIGVVISDHNVRETLGVCDQAYILNEGRIL
                     EKGTPEEIAGSRKAREIYLGENFQF"
     gene            2645..4069
                     /gene="rpoN"
                     /locus_tag="VEL68_18835"
     CDS             2645..4069
                     /gene="rpoN"
                     /locus_tag="VEL68_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXZ38088.1"
                     /translation="MALEIRQSLKLAQQPIMTPQLQQAIKLLQLSRMELMDLIRQEQE
                     ENPVIEESVEPAAEAEPAEGGYETQGPSSSDSKEIRQQEIGPPEADWREEPYWRTKPT
                     EDEEDRPTFENFLTKKTSLSDHLLWQLRLNHFSEDERLIGTLIIGNLNEDGLLQVSLE
                     EIGAQSGFDLETVQKVMQKVQTFDPPGVAARDLRECLLLQAQQLFPQELLMQRIISSH
                     LNILAKKSYQSLTRELGVPLEEIIRSTRLISELDPRPGRRFSDDNIPYITPDVYVFKM
                     GDEYVVMLNEDGLPRLRINSYYRNILREQAKFSGEAKDYINDKIRSALWLIKSIHQRQ
                     RTISRVTKSIVKFQREFLEKGVAYLKPMVLKDVALDVEMHESTISRVTSNKYVHTPQG
                     IFELKYFFNSSINTSRGENVASESVKEKIRLILAQEDPQKPFSDQELVEVLKKQDILI
                     ARRTVTKYREMLGVLPSTQRKQII"
     gene            4092..4637
                     /gene="raiA"
                     /locus_tag="VEL68_18840"
     CDS             4092..4637
                     /gene="raiA"
                     /locus_tag="VEL68_18840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXZ38089.1"
                     /translation="MQVSVTFRNMESKEIAREYVQEKIAKLKKYLDTPLEANVILTTA
                     KHRQIAEVNLLANRLTINAREETEDMFAAIDGVMDKLERQLSKYKEKIKRRKVNSNLP
                     ESSWQMNVFSSESFEEGASPKLIKSKRLLIQPMSVEEAALQLELLNNEFYIFTNVESK
                     SLNIIYRMKDGHYGLIEPQGG"
     gene            4769..5230
                     /locus_tag="VEL68_18845"
     CDS             4769..5230
                     /locus_tag="VEL68_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXZ38090.1"
                     /translation="MKISDILGRDRIVPQLSSQTKEGILRELVHALAKGAQQVNEGRM
                     IEVLLERESLGSTGIGEGVAIPHGKSKEVKEIMATFGRSLSGMDFQSLDGKLTHLFFL
                     LIAPENSAGTHLKALAQVSRLMKDPSFRKRLMEARTSDDIYAIFSEGDEKG"
     gene            5329..6213
                     /gene="rapZ"
                     /locus_tag="VEL68_18850"
     CDS             5329..6213
                     /gene="rapZ"
                     /locus_tag="VEL68_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXZ38091.1"
                     /translation="MGSPSDLKHLRIVIITGLSGSGKSTASRALEDMDFFCVDNLPIA
                     LLPKFLDLSSASMGEYSKIALVMDLREKEFLRTYQEIFSRLGAEGYQLEILFLEATDE
                     VLVRRYSQTRRTHPLADGLGLVEGIQKEREMLAPLRSMATLVMDTSTFNVHQLQLAIR
                     ELFGQRPPGRRMALTFLSFGYSQGIPQEADLVMDVRFLPNPFFVEGLKDLPGSDSRIY
                     DFLMSFEGSREWFERFEHFLAYLLPLYEREGKAYLTVAIGCTGGKHRSVAVAERMKAI
                     FQGEFPVRVRHRDLKNSP"
     gene            6252..6656
                     /locus_tag="VEL68_18855"
     CDS             6252..6656
                     /locus_tag="VEL68_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXZ38092.1"
                     /translation="MVGVVLITHPNLGGEFIRSAETICGKLPFLASVSIDTRKGVEEL
                     REEITRAIKTVDSGKGVLILTDMFGGTPSNMSLAFLSEGRVEVVTGLNLPMLIKISNC
                     REGRTLQELAKMVKEAGQRNINLASEILKKKS"
     gene            6728..7207
                     /gene="rimI"
                     /locus_tag="VEL68_18860"
     CDS             6728..7207
                     /gene="rimI"
                     /locus_tag="VEL68_18860"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXZ38093.1"
                     /translation="MTERDLDEVLEIERESFPTPWSKGLFAKELAIPFARAFVAREKD
                     SGKIVGYLCFWLVDQETHILNLAVHPRIRRQGIGTRLLAFGISYCQRQGVQQITLEVR
                     RSNLKAISLYKNFRFQPQGVRRRYYTDSGEDAIIMGLDLEKEKSLAPYEPPSQRSEG"
     gene            7176..8153
                     /locus_tag="VEL68_18865"
     CDS             7176..8153
                     /locus_tag="VEL68_18865"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HXZ38094.1"
                     /translation="MNPRPREVKAKILLHQRIGSDYFRLQLACPEIARLARPGQFIML
                     RITDLKDPFLRRPFSFSRIFPPLEVKKNPLDEGGVEICYQTVGRGTRLMTQLQEGQRI
                     DLLGPLGNGFWPEDRCTRVILVGGGIGVPPLLSWAQGLRKKGSGKKRSGKASEGSPEM
                     IFLMGAKSKEKIMGVGECRKWGIEVQVATEDGSLGAKGMVTDLLERELMTGQHGSTAL
                     YACGPNPMLVQIAQVAEQFDRPCQVLLESRMACGVGACLGCTVKYREEREPIQFSAHS
                     SQDSPAGEEGEEKENREGAMPMISETPPFRYARVCKEGPVFDARKIFWD"
     gene            8162..9118
                     /locus_tag="VEL68_18870"
     CDS             8162..9118
                     /locus_tag="VEL68_18870"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HXZ38095.1"
                     /translation="MGSKKEGPKISVELGPLKLKNPVMAASGTFGYGEEYSGIISLRN
                     LGALITKGLSLKPRRGNPPPRLAETPAGMLNAIGLENVGIKAFIGEKMPFLRKAGCPV
                     LVNIFGATDEEYAEIARRLSEVEGVAGLEVNISCPNLKAGGCLFAAEPKAVFQVVSRV
                     RQASPCFLMVKLSPNVGDIVPVALAAEEAGADALSLINTLTGMAIDIRTRTPILGNIT
                     GGLSGPAIKPIGLRMVWQAAQAVRIPVVGVGGIGCAEDALEYMIAGASAVQVGSAHFI
                     DPRAGLKIVDGIHDYLKTQGIPDLSSLVGSLKAGQDNRKTDR"
     gene            9242..9727
                     /locus_tag="VEL68_18875"
     CDS             9242..9727
                     /locus_tag="VEL68_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HXZ38096.1"
                     /translation="MELRSVTLEIPEGTNIIFGQTHFIKTVEDLYEIMAGSVPQARFG
                     IAFCEASGACLIRVEGNDQELRKVATRNAQAVAAGHTFVLLLRDAYPINVLNAIKGCA
                     EVCSIYCATANPVEVILAESSQGRGVLGVIDGFSPKGVEGPADLQWRKDLLRKIGYKI
                     S"
     gene            9934..11925
                     /locus_tag="VEL68_18880"
     CDS             9934..11925
                     /locus_tag="VEL68_18880"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HXZ38097.1"
                     /translation="MEPGKVVVFFEQKKVLLAVCLEVKGTKAHLLSEENREVTLGFNR
                     IVHTSASKLNLVSPRETLLEQLKAAEARQRKLMESLAVRDLWDLVWEERREFSLPELA
                     ELVFHPPVPFDEEMALFRALFDDRLYFKQKGGSYEAREPEKVEEIARQIEREAEQERE
                     LRESSEWLARVWAGEAALPFAGKEKVIGLLKDYVLFGSESPDLTRAKALLEAAQITSS
                     EAPFLLLVRLGVWAEDENLFLHRHQISQVFPERVIVEAERILTFSSKEIPPQPQDCDL
                     TFLHPFTIDSEFTRDIDDALSLERVGDEYQVGVHITDVATFLNGSGEIFQEAMARAIS
                     IYLPDQRIPMIPPALSEGACSLIVGEKRRALSFLVRVDRGGKVLDSKIVPSIIQVERR
                     LSYEQVDQLLEEMEEELSLLKQVSEHLGRRRMELGAFFLPRPERVIRVTRDREIIMYK
                     RDRESPSQKMVAEFMILANMLAALFLRERGIPAVYRGQMEPRERIPPMEHFDVLQAYR
                     LRRVMNRVEIGTRPLRHAGIGAEAYLTLTSPIRRFYDLLVEHQILGSLRGTPILSQEE
                     VEEIITRVGPILSKVGLVEELTEQYWIKRYLEKKIGSTTTAVVLDRWPNRYLIYLEEY
                     LLEVDMPAIPGREFIPGDQVLVRIEKSNARQGILKIVPV"
     gene            complement(12315..13751)
                     /locus_tag="VEL68_18885"
     CDS             complement(12315..13751)
                     /locus_tag="VEL68_18885"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HXZ38098.1"
                     /translation="MKLNMTLLTDLYQLTMVGAYYLLGKSQQKANFDYFFRKIPEDGG
                     FCVAAGLEQLTEYIENLHFEPEDLDYLRSLNLFPPEVLTFFQNLRFTGDLYAVPEGTL
                     VFPQEPIIRVTAPLPEAQFIETALLNILNYQTLIATKAARVCIVASEDPVIEFGVRRA
                     QGPDGGLSASRAAFIGGARATSNLMAGKVFGIPVRGTVAHSWIESFPSELESFQSYAK
                     VYPKNVVLLVDTYDTLLSGVPNAIKVGNELRARKDGDLLGIRLDSGDLTFLSREARKL
                     LDEAGFDRTRILGSSDLDEWLIESIKKQGAEIDLWGVGTRMVTSYSCPALGGVYKLSA
                     ILEDGEMKPKLKVSDDPEKTTNPGIKKVVRFFDEKNFMRGDVLFFEDEILPENRPIQA
                     FHPMLAHVHKTYPSRYKREEILVPIFQGGKRVYSLPPLADIQTRTLQNLHYLRPEHKR
                     LQNPHIYHVSLGERLFKQKQDLIQAAAG"
     gene            complement(13892..15067)
                     /locus_tag="VEL68_18890"
     CDS             complement(13892..15067)
                     /locus_tag="VEL68_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ38099.1"
                     /translation="MDSLIEMGPIRPPSEAQSLLIRVTRNCPWNQCEFCHTYKGETFS
                     IRSLEEVQKDIDQAKEIADQIWQLSWRMGQAGKIDQTLLHHFLSQPKAQNPAWQTVAG
                     WLYFGGSQVFLQDGNSLMRKTGDLVAILRYLREKFPSIQRITSYGRARTLAKKSVKEL
                     KELREAGLTRIHIGMETGYEPLLQYIKKGVTAEEQITAGRNVVASGISLSEYIMPGLG
                     GERWWREHAVESARVLNQIDPDFIRLRTLYVRKNMPLYQKVEKGEFVRPSDDQIVIEI
                     RLFLETLDGIHSTVVSDHILNLLEEIQGRLPEDKEKMLALIDRYLAWPKKKRFQFRVG
                     RRLGYLRNLDDLDDQNLLLRVDQIIQKVQRSIREEQGKDVPEQEIEKEIHALMENYI"
     gene            15281..16522
                     /gene="amrB"
                     /locus_tag="VEL68_18895"
     CDS             15281..16522
                     /gene="amrB"
                     /locus_tag="VEL68_18895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXZ38100.1"
                     /translation="MADALDYPRIRSVDAFPAEVSGQKVVCLRDPLGLSGKVLFFPYP
                     TFFIISLFDGSHSLVDIQAEFMRHFGELLYREKIQELIAQLDEHFLLDSERFRDAEQK
                     IIDGFQSSPLRSLTLTEDSYGGGAEKLKETIESYFLDPEGPGPPAKKRDSRFLVGAIA
                     PHIDYRRGGPCYAWAHRAILESSPADLFVILGTAHSAMKQPFALTRKHFQTPWGTVET
                     DQEFMDTLDREVSGDFYQDEFAHKGEHSIELQLVFLKALWPKTDPFRIVPILCGSFHE
                     AILQDESPMELPGVASLIQALKKAMAGAKRRVCALASADLAHVGPRFGDPIVPDRISL
                     QNLAEEDRRLLGYAEGVDGEGFFRILAREKDRRKVCGLSPIYVLLHLLEGARGKFLKY
                     SQSLDSTTQSVVTFGSVAYYS"
     gene            16653..16943
                     /locus_tag="VEL68_18900"
     CDS             16653..16943
                     /locus_tag="VEL68_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38101.1"
                     /translation="MKRKVVLKSPVYWVLGGLAAGIFLTGFFWLREPTRAFGGNFAPM
                     AQQQFARFQISGRDANSAWVIDTAAGDVFLIFSNGKWKEVGSIFDEKSRLKN"
     gene            17107..18192
                     /locus_tag="VEL68_18905"
     CDS             17107..18192
                     /locus_tag="VEL68_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38102.1"
                     /translation="MAKEWRLLDLYVDLNENSAFLNPAIQRARMEGLVPDTVIFCSIK
                     KIGISLGFYPDPEKDINLEYCRTHDIGVSRRLQPYYVNIGYPGFIVVYIIAGKNFMPP
                     TVAEIFKTVISQMAEEFNGRWGTAAQYRPFNDMEVGGRKISWMGGSFFGDVWSGASVL
                     LVKPSPIDKINPALNIPPEKFADKDAKTAGERAVSLEELAGRPIPLEEVREAYRQSLE
                     TTFGVKLVLGCLSEMEMKYEQENREKYSSEILLARTEGKKFGPVAPGVKRGEAIWKVP
                     RGGLVRAVARVKEGTIENLLFSGNIYCSPVTALEEVEGLLKGVPLEEDQIRERVKVIY
                     GKPNYQLTGTTPEDLARLVLEAGRKAI"
     gene            18220..18516
                     /locus_tag="VEL68_18910"
     CDS             18220..18516
                     /locus_tag="VEL68_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38103.1"
                     /translation="MSEESKGTISEEEFVYRAIKRLRKPPYRGIHSVFSGFNQAFKEH
                     FGKNPIETTQKMAGEGKIVTRPVKGGVMLYLPGDAPQPQKGVLQKILGPEEESA"
     gene            18663..19943
                     /locus_tag="VEL68_18915"
     CDS             18663..19943
                     /locus_tag="VEL68_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38104.1"
                     /translation="MAKKENLQARLRISGKQLDAINDLLLDPKSRVVKDFLAVVAKYG
                     TPEEINAKAAEAGKLENLMDRLEKEKSPYLADVKWMIAQRDAKAFVSVAEFRKSLLGE
                     KAARMKFKEKFAVTLEISAAQYFPWVIEEARQAIAKRELMPGRFIRVRKMKEQEGDNG
                     DILAFAAAMQVMGASFVETLDTKGTDGSNVHLGGPDTITGYFGGIGQPNEYPLKWVDE
                     FLYYFTNYGIRQVLNINPGTVFLGYMLGKLGIQNEFKISVFMGNDNPYAALWTLIGGK
                     LFSAKDGSCPLIGFNLSNSVNNQTIERVAEVRNKLGFEDIVRIEHHITETWKSIVRQP
                     YNRRDELVPLAGHVANIAAKHEGSEIETEKGRQHPSDIFDYFREKKEIEASGDMPALL
                     QNYLDKHDSVNLTARALTEKGLSFKAASNLHHRK"
     gene            20144..>20833
                     /locus_tag="VEL68_18920"
     CDS             20144..>20833
                     /locus_tag="VEL68_18920"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXZ38105.1"
                     /translation="MEAEKGRAERFALAAEKYVPDAITSAVIMVIILFLYAWSIGVPA
                     DKTTDAFYRGFWMLLAFTMQMTLIITLSAVFGQSPIFRKAIMALAKVPNSPFQVVILA
                     ALATGVLGYFYWGLAITLGPVIAVYFCGEAERKGIKVDFMLTLAVVFAAHSVWQYGLS
                     SSAPLLSATPGHFLEKLTGKMPLSSTIWTPAAIFQIVAFMVILLIVARYLVPRVPKTI
                     SEYPEAFKLIEA"
BASE COUNT         4821 a         5433 c         6102 g         4477 t
ORIGIN      
        1 gaatggtacg gaaaggctgg aaagaaaggg cgaaaaaagt ccgcctcctg cttttggatg
       61 tagacggcgt catgaccgat gggcgtctgg cgtttgatgg ggaggggaaa gaacttaaat
      121 ttttctatgc ccgggatggc atcgggatta aactccttca ggaagccggg attcgggtgg
      181 ggatcctttc gggaaggagg gcaaaagtgg tggccctccg ggccaaggag ttgggaattt
      241 ccctggtccg gcaaaagaca agagataagg cccaggcctt ggaggaaata ttggaggccg
      301 agaacctgcg gccagaacag gtctgctacg tgggcgatga cttggtggac ctttccgtcc
      361 tccggcgggt gggattgccg gtggcggtgg ccgatgcggt gccagaagtg aaattcatgg
      421 cccgtctggt tacccgaaat ccggggggcc ggggggcggt gcgggaggtt tgcgagaaga
      481 ttttgaaaat acagggaaaa tgggagaaag gaatgcgaaa attccgctcc tccccggcgg
      541 ggtgaaacgg ttatatccgc cccctggatg attccagccg ggagaggtgc cgatggcccg
      601 ccggcgaatc cattcagccc aaaatgaccc tttacaccct cccaagggcg atgttagaat
      661 gaccttggag atgggaggga tgagaagaag catcactctt cggtcactga taggggtgat
      721 cattcttggg agtttgggcc tcgtgggggt tttggtctgg caagccttga ctctccgctc
      781 cacggaaaaa atctctcctt ccaagccgga cgcttccgcc gacctgaggc tggacagggt
      841 tcattacacc gagacccggg agggagtgaa agaatgggaa atggaggcca cctccgcggt
      901 ttaccgcaag gagggggata cgatggtttt ggagaaaatt cgggccacct tttacgggaa
      961 gaatcaggag atttaccatc tggtcggaga gaagggaaag ttcaacaccc agaccaaggt
     1021 gattgaagtc tacgatggag tcacgatcga ttccgacgac ggctaccata tgaggactcg
     1081 aagcttgaaa taccaggccg atcagaaaga actccacacc tccgacccgg tggaactgga
     1141 ggggccatcg atgcttgtgg aaggggtggg gatggtcgtg gatttgaacc gcgagcgcgt
     1201 gaaaatcctg agggatgtga caaccaccct tccttccctg gattggaaga agagcgtaag
     1261 gtcggccagg tgatatgaaa agcaagggca tcgtatgggc catgggggtt ttcttcttgc
     1321 tcctggcccc gcttcttccc gggtgggctg cagagaaaaa gccggccctc cttggaaaag
     1381 ccacccagga agggtctgct caacccctcc gtatcacctc ccagcagctc gaggccgaca
     1441 acaaaagcca ggtgatcacc tttaccggaa acatcatcgc ccggcagggg gacatggtca
     1501 tttatgcgga cgtcgcccgg gtctattatg agaaacggga agaggggaat gaagtccggg
     1561 agatcgtagc cgaggggaac gtgaagattc atcaggggga tcgggtggcc acgggcgagc
     1621 gggcgcagtt cctgaacgga gagcagaaga tcattctaac cggacaaccc cgggtctggc
     1681 agggaaagga tatggtcagc ggggaaaaga tcacggtttt gctggaagaa gataaaagcc
     1741 ttgtggaaag cggcccggac cggcgagtgg aggtaatttt gtatcccaag ggggaggcag
     1801 gcaaggcaaa aggaaaacca tgagttcggt ccagaccctt cttgcccggg gattgaagaa
     1861 gcagtataac agcaaatggg cggtggactc catcgacctg gaattccggt cgggggaggt
     1921 gatcggcctt ttggggccga acggagccgg taagactaca accttttaca tcatcgtggg
     1981 gctcattcgg ccggatgggg gggagatttt cctttccgga gaagagatca cccatgatcc
     2041 catgtacctg agggcccgga agggggtcac ctatctcccc caggagccgt cggttttccg
     2101 taaactgacc gtagaggaaa atctgatggc cattttggag tcgttggatt taacccaaga
     2161 agaaaggcag aagcgcctga agacattact ggaagacctg aggatctccg gtctggccaa
     2221 acgaaaagcg tactcccttt ccggggggga gcggagaagg gtagagatca gccgcagcct
     2281 ggtgacttcc ccccgtttta ttctttttga tgaacctttc gccgggatcg accccctggc
     2341 ggtggtagac atccagaata ttatcgtcca gctcaagtcc agaggaatcg gtgtagtcat
     2401 atcggaccac aatgtccggg agaccctggg agtatgtgac caggcctata ttctgaatga
     2461 gggacggatt ctagaaaagg ggacccccga ggagattgcc gggagccgga aagcccggga
     2521 gatttacctg ggagagaact ttcaattcta aaagattgaa ggattccgtc caaaaggtcg
     2581 ataaaaaaag agaaaagggt aaaaactcaa aggcttcctg ccaccccaag gacatgattt
     2641 tcttatggca ttagaaattc gacagagcct caaactggct cagcagccca ttatgacccc
     2701 tcaacttcag caggcgatca agctgctgca gctttcccgg atggagctga tggacctgat
     2761 ccgacaggag caggaagaaa atccggtgat tgaagaatcc gtagagccag cggcggaggc
     2821 agaaccagca gagggcggtt acgagactca aggtccttct tcttcagaca gcaaggagat
     2881 tcggcaacag gaaatcggtc cgccggaagc cgattggagg gaggagccct actggaggac
     2941 caaacccacc gaggatgaag aggaccgtcc cactttcgag aacttcctga ccaagaagac
     3001 cagcctctcc gaccaccttc tctggcagct tcggctgaat cacttcagcg aagatgagcg
     3061 gctgatcggg accctgatca tcggcaacct gaacgaggac ggcctcctgc aggtttccct
     3121 ggaagaaatc ggcgctcaga gcggttttga cctggagacc gtccaaaaag tgatgcaaaa
     3181 ggtccagacc tttgacccgc ccggagtggc cgcccgggac ttgcgggaat gcctgctcct
     3241 gcaggcccag cagctctttc cccaagagct gctcatgcag cggatcatta gctcccattt
     3301 gaacatcctg gccaagaaaa gctatcaatc gctgacccgc gagcttgggg ttcccctgga
     3361 agaaatcatc cggtccaccc ggctcatcag cgaactcgac ccccgcccgg ggcgacggtt
     3421 cagcgacgac aacattccct acatcacccc cgatgtgtat gtttttaaga tgggggacga
     3481 atatgtagtc atgctgaatg aggatggcct tcccaggctg cggatcaact cctattaccg
     3541 gaatatcctt cgcgagcagg ccaagttttc cggagaggcg aaggactaca tcaacgataa
     3601 gattcgctcc gccctctggc tgatcaaatc cattcaccag cggcagcgca ccatttcccg
     3661 ggtgaccaag tccatcgtga aattccagcg agaatttctg gaaaaaggag tggcctactt
     3721 aaaacccatg gtcctcaagg acgtggctct ggacgtggag atgcacgagt ccaccatcag
     3781 ccgggtcact tccaacaagt atgtccatac gccgcagggc atcttcgagc tcaagtattt
     3841 cttcaacagc agcatcaata cctcccgggg ggagaacgtg gcctccgaga gcgtcaagga
     3901 aaagatccgt ctgatcctcg cccaggagga cccccaaaag cccttcagtg atcaggagtt
     3961 ggtggaggtc ctgaaaaagc aggacatcct catcgcccgc cgaacggtga ccaaataccg
     4021 ggagatgctg ggggttcttc cttccactca aaggaaacaa atcatataat tctcggatgg
     4081 aggcgccggg catgcaagtt tcggtcacgt tccgtaacat ggaatcgaaa gagatcgcgc
     4141 gcgaatacgt acaggagaag attgccaaac tgaaaaaata cttggacacc cccctggagg
     4201 ccaacgtcat cctaaccacg gcgaaacacc gccagatcgc agaggtcaac ctgttggcca
     4261 accgcttaac catcaacgcc cgggaagaaa ccgaagacat gttcgcggcc atcgacgggg
     4321 tgatggacaa actggagcgg cagctctcga agtacaagga gaagattaag cgtcgcaagg
     4381 tgaactccaa cctgccggaa tccagctggc agatgaacgt cttttcttca gagagttttg
     4441 aggaaggggc ttctccgaag ctgatcaaga gcaagcggct gctgatccag cccatgtccg
     4501 tggaggaagc ggctctccag ctggagctct tgaacaatga attttacatc tttaccaatg
     4561 tggaatccaa aagtctgaat atcatctacc ggatgaaaga cggccattac ggcctgatcg
     4621 aaccccaggg cggatgagtt tgcctccggc ctccaagccg aatcggggga acgaaaggac
     4681 ggtgccaagc cgagggtcaa gggaaatcca cggggcactt ctccccaaga agggggggag
     4741 ggaggattcc tccaaactct cggagctcat gaaaatttcg gacatcttgg gtcgggatag
     4801 gatcgttccc cagctttcct cccagaccaa ggagggaatc ctgcgggagt tggtccacgc
     4861 gctggccaaa ggggcccaac aggtcaacga ggggcgcatg atcgaggtcc tgctggaacg
     4921 agaaagcctg gggagcacgg ggattgggga aggggtggcc attccccacg gaaagagcaa
     4981 agaggtcaag gaaatcatgg ccactttcgg gagaagcctg tcgggcatgg acttccagtc
     5041 cctggacggg aaactgaccc atctgttttt tcttctcata gcgccggaga attcggccgg
     5101 aacccatctg aaagccctgg cccaggtctc ccgtttgatg aaggacccat ccttccgtaa
     5161 gcgcctcatg gaagcccgca cctcggatga tatttacgcc attttttccg agggagatga
     5221 aaaggggtaa aggaagtctc tgaggcaggc cgaccttgaa cggtttcggg tttcttgcga
     5281 tcctccccgg cgcattaccg aagaaacgaa aggggaagct tcctggccat gggaagccca
     5341 tccgacttga agcatttgcg gatcgtgatc atcaccggat tatccggttc gggcaagagc
     5401 acggccagcc gggccttgga ggacatggat tttttctgtg ttgacaacct gcccatcgcc
     5461 ctgcttccca agtttctcga cctttcctcg gcttccatgg gagagtattc caaaatcgcc
     5521 ctggtgatgg acctgcggga gaaggaattt cttcggacct atcaagaaat tttttcccgc
     5581 ttgggggcgg aggggtatca gctcgagatc ctttttttgg aagccaccga tgaagtcctc
     5641 gtccgccgat acagccagac gcgacgcacc catcccctgg cggatggact tgggctcgtg
     5701 gaaggaatcc agaaggagcg cgagatgctc gctcccctgc ggtccatggc aaccctggtc
     5761 atggatacct ccaccttcaa tgttcatcag cttcagctgg ccattcgcga gctctttggg
     5821 cagagacccc cgggacgaag gatggccctg accttcctat ctttcgggta cagccagggg
     5881 attccgcagg aagccgatct ggtgatggac gttcgcttct tgcccaatcc ctttttcgtg
     5941 gaagggttga aggatttgcc ggggtccgat tcgcgaattt atgatttcct catgagcttt
     6001 gagggttcgc gggaatggtt cgagcgattc gagcattttc tggcttatct tctccccctg
     6061 tatgaacggg aaggaaaagc ctacctgacg gtggccatcg gttgcacggg gggaaagcat
     6121 cgatcggtgg ccgtggccga aaggatgaaa gccatctttc agggcgagtt tccggttcgg
     6181 gtcaggcacc gcgacctgaa gaattctccc tgacggatga tttcaaaaga agggatagaa
     6241 gtgggggggc catggttggc gtggttttga ttacccaccc gaatttggga ggagaattca
     6301 tccgttccgc agagacgatt tgcgggaaac tcccatttct ggcgagcgtt tccatcgata
     6361 cgcgaaaagg ggttgaagaa ctgagggagg aaatcacccg ggccatcaag accgtggatt
     6421 ccggcaaagg ggttttaatt ctgaccgaca tgttcggggg cactccttcg aacatgagcc
     6481 tggcgttttt gagcgagggg cgcgtcgagg tggtcaccgg cctcaacctt cccatgctca
     6541 tcaaaatatc caactgccgc gaaggacgga cccttcagga gttggccaag atggtaaaag
     6601 aggcaggcca acgcaatatt aatctggcca gcgagatttt gaagaagaag tcctgacctc
     6661 aggcagaatc cctacgaacc caaaacacga tcccgtggaa gggcaccagc cgatcgaaat
     6721 ccaggaaatg accgagaggg acctggatga ggttttggag attgagcggg agtcatttcc
     6781 aaccccctgg tcgaaggggc ttttcgccaa ggagctagcg atcccttttg cccgggcttt
     6841 cgtggcccgg gaaaaggatt ctggaaagat tgtggggtat ctctgcttct ggttggtgga
     6901 tcaggagacc catattttga acctggcggt ccatccccgg atccgccggc aagggatcgg
     6961 aacccgcttg ctggcctttg gaataagcta ttgccaaaga caaggagtac agcagattac
     7021 cctagaagtc cggcgatcga acctgaaagc gatttccctg tacaaaaatt tccggtttca
     7081 accccaaggg gtccgtcgca gatactacac cgacagcgga gaggatgcga tcatcatggg
     7141 attggacctg gagaaggaga aatccctggc cccctatgaa cccccgtccc agagaagtga
     7201 aggctaaaat tctcctccat caaaggatcg gttcggatta tttccggctt cagctggcct
     7261 gcccggaaat cgcccgcctg gcccgcccgg gacagtttat catgttgcgc atcacggatt
     7321 taaaagaccc cttcttgcgc cgtcctttca gcttctcccg gattttcccg cccctagagg
     7381 tgaagaagaa ccccctggac gagggagggg tggaaatctg ctaccagacg gtgggtcgag
     7441 gcacccgtct catgacccag ctccaggagg ggcagaggat cgatctgctc ggccccttgg
     7501 ggaatgggtt ctggcccgag gacagatgca cccgggtgat cctggtggga ggagggatcg
     7561 gagtgcctcc tcttctctcc tgggcccagg gattaagaaa gaaggggtcc ggaaagaaaa
     7621 gatcgggcaa agcctcggaa ggctccccgg agatgatctt tttgatgggt gcgaagagta
     7681 aagaaaaaat catgggggta ggggagtgcc ggaagtgggg aatcgaggtc caggtggcca
     7741 cggaggacgg gagcctgggg gcgaaaggga tggtcaccga ccttctggag cgagaactga
     7801 tgaccggaca gcatggttcc accgcccttt acgcttgcgg tcccaatccc atgctggttc
     7861 agatcgccca ggtggccgag cagttcgacc ggccgtgcca ggttctgctg gagtctcgga
     7921 tggcctgcgg agtgggagcc tgtctgggct gtacagtaaa atatcgggaa gagagagagc
     7981 caatccagtt ctcagcccac tccagccagg actcccctgc cggggaagaa ggggaagaaa
     8041 aagaaaaccg ggagggggcg atgccgatga tttccgagac tccccccttt cggtatgcgc
     8101 gggtctgcaa ggaaggcccg gtttttgatg cccgtaaaat tttttgggat tgaggaaatc
     8161 catgggttcc aaaaaagaag ggcccaaaat atcggtcgaa ctcggccctc tcaagctgaa
     8221 aaacccggtc atggcagcat ccggaacgtt cggatatggg gaagagtact cgggaatcat
     8281 ttccctgaga aacctgggag ccttgattac caaaggcttg tcgctgaaac cacggagagg
     8341 aaacccccct ccgcgtcttg ccgaaacccc tgcaggaatg ctgaacgcca tcggtttgga
     8401 gaatgtcggg atcaaagctt ttatcgggga gaaaatgcct tttctgcgca aagccggctg
     8461 cccggtcctg gtcaacatct ttggcgccac cgacgaggaa tatgccgaaa tagcccgaag
     8521 gttgtcggag gtggaaggag tcgcaggtct ggaggtgaac atttcctgcc ccaacctgaa
     8581 ggccggggga tgcctattcg ccgctgaacc caaggcggtc tttcaggtcg tctccagggt
     8641 tcgtcaagcc tccccctgtt ttctgatggt caagctctct cccaatgtgg gtgatatcgt
     8701 gccggtggcc ctggctgctg aagaagcggg ggccgatgcc ctctccttga tcaataccct
     8761 gaccggcatg gccatcgata tcaggacccg gactccaatc ctgggaaaca tcaccggggg
     8821 gctgtccggc ccggccatca aacctattgg cctgcgcatg gtctggcagg cggctcaggc
     8881 ggttcgcatt ccggtggtgg gggtgggagg gattggttgc gccgaggacg cgctggagta
     8941 tatgattgcc ggagccagcg ccgtccaggt cgggtcggct cacttcatcg atccccgagc
     9001 tggtttgaaa attgtcgatg ggattcacga ctatctcaag acccaaggaa tcccagacct
     9061 ttcttctttg gtgggaagcc tgaaggctgg gcaagataac cgcaaaactg atcgttgatc
     9121 ctggaccctg ggtcccggat atcggatagc agatccttga tcctaagccg gggatttttt
     9181 tatccagcac cgtgtatccg gaatctagaa tttgtatccg aaatcaggaa gggggctatt
     9241 catggagctt aggtcggtta ccctggaaat tcccgaaggc acaaatatca ttttcggcca
     9301 gacgcatttc atcaaaacag tggaggacct ttatgaaatc atggccggct ccgtgcccca
     9361 agctcggttc ggtatcgcct tctgcgaagc ctccggcgca tgcctgatcc gggtggaagg
     9421 aaatgaccag gagttaagga aggtggcaac gcgaaacgcc caagcagtgg ccgcggggca
     9481 taccttcgtc ctgctcctgc gcgacgcgta tccgatcaat gtattgaatg ccatcaaggg
     9541 ctgtgccgag gtctgttcca tctattgcgc aacggccaat ccggtggagg ttatcctggc
     9601 cgaatcttcc caaggtcgag gggttttggg ggtgatcgat ggcttctctc ccaaaggagt
     9661 ggaaggacct gccgaccttc agtggagaaa agacctcctg cggaagatcg ggtataaaat
     9721 ctcctaaatg ccggtcgcta gttgctcatc actggttact cgttacccgt tattgtctaa
     9781 gaagacttcc tctaacatgg aaccagaaac gagcaaccgg caaccaatcc tgcctcttga
     9841 tttccttctc gcgcggatgt tatgttggtc aagatgccga atcatagccc cttctctttt
     9901 cccttctcgg gggaaggtta gaaatgagta tggatggagc caggaaaggt cgttgtattc
     9961 tttgaacaaa aaaaggttct cctcgccgtc tgcttagagg tgaaggggac caaagcccac
    10021 ctcttgagcg aagaaaaccg agaggtgacc ctgggcttca accgtatcgt ccacacctca
    10081 gcttccaaac tgaaccttgt ctctccccgt gaaacgttgc tggaacaatt gaaagccgcc
    10141 gaagcccgac agagaaaact catggagtct ctggccgtcc gggacctctg ggatttggtt
    10201 tgggaggaaa gaagggagtt cagcctgcct gaactggcag agctggtctt tcacccgccg
    10261 gttccgtttg acgaggaaat ggccctcttc cgggcgctct tcgatgaccg cctctacttc
    10321 aagcagaagg ggggaagcta cgaggcccga gaaccggaga aagtcgagga aattgcccgt
    10381 cagatcgagc gagaggcaga gcaggaaagg gaactccggg agagcagcga gtggctggcc
    10441 agagtatggg cgggagaggc agccctcccc tttgccggca aagaaaaggt cattggcctc
    10501 ctgaaggatt atgtcctttt tggttccgag tccccggacc taaccagggc caaagcactt
    10561 ctggaagcag ctcagatcac ctcctccgaa gctcctttcc tgctgctggt ccgtttggga
    10621 gtctgggcgg aggacgagaa tctcttcctc catcgccatc aaatttccca ggtcttcccc
    10681 gagagggtga tcgtggaggc cgaacggatt ttaaccttct cctcgaaaga aattcctccg
    10741 caaccccagg actgcgactt gactttcctc catcccttca ccatcgacag cgagttcacc
    10801 cgggacattg acgatgcttt gagcctggag cgagtggggg acgagtacca agtgggggtc
    10861 catatcaccg atgtagccac cttcctgaat ggaagcgggg agatttttca ggaagccatg
    10921 gcccgggcca tttccatcta ccttccagac cagcggatcc ccatgattcc cccggctctc
    10981 tctgaagggg cctgcagcct gatcgtgggc gaaaaacggc gcgctctgag cttcttggtt
    11041 cgggtcgacc gaggggggaa ggtcctggat tccaaaatcg ttcccagtat tattcaagtg
    11101 gagcggagac tttcctatga acaggtcgat caactcctgg aggagatgga ggaagagttg
    11161 agccttctaa agcaggtctc cgaacacctc ggacgtcggc ggatggagtt gggcgccttt
    11221 ttcctccccc gtccggagcg ggtaatccgg gtcacgcgcg accgggaaat tatcatgtat
    11281 aaacgggacc gggaaagccc ctcacagaag atggtcgcgg agttcatgat cctggccaat
    11341 atgctggccg cgctttttct ccgagaaagg ggcattccgg ccgtttaccg ggggcagatg
    11401 gagccgcggg agagaatccc tcccatggag catttcgatg tccttcaggc ctaccgcctg
    11461 cgacgggtga tgaaccgggt ggagatcggc accaggccct tacgccatgc aggcatcggg
    11521 gccgaagcct atctcaccct gacttccccg attcggcggt tctatgatct cctcgtggaa
    11581 caccagatcc tgggaagcct gcgagggact ccgatcctga gccaggaaga ggtggaagaa
    11641 atcatcaccc gggtggggcc cattctttcc aaagtgggct tggtggaaga gctcaccgaa
    11701 caatactgga tcaagcgcta cctggagaaa aagatcggat cgacgaccac tgccgtggta
    11761 ctggaccgtt ggcccaatcg gtacctcatt tacctggaag agtatctcct ggaagtggat
    11821 atgcccgcca ttccggggag agaattcatc cccggggatc aggtattggt ccgcatcgaa
    11881 aaatccaatg cccgccaggg aatcctaaag atcgtgccgg tctgacctag tgtcgcgtct
    11941 cagaaataag ctgcataatt ctgccgccga gcagggaggg gggcgaagaa ccgctgcgct
    12001 taccaaaata agaagctcgt tcctctttga aaaagttgat tcttactaaa ggtttcggaa
    12061 gttgtccatt tttgcgctct tggcctaatt tcccggaggc cggcagcgtc gctttgcgga
    12121 taccaggcca ccgctcggag acaggccatt tcccggccga ttttggcggc gcgcatcgtt
    12181 tctccgcccc cctcctggta aatttattca ctgaacttag gggacgcgac actagttccc
    12241 agttggtcgg tcctggtttg tggtttaagg gaatttttct ttgacaagca acgagcaacc
    12301 aacaagagtc tttcttatcc cgccgccgct tgaatcaggt cctgcttctg tttgaaaagt
    12361 ctttcaccca gactgacgtg atagatatga gggttttgga gtcgcttgtg ctcggggcga
    12421 agataatgaa ggttctgcag ggtcctggtc tgaatgtccg ccaggggagg aagagagtaa
    12481 acccgcttcc ctccctggaa aatgggcacc agaatctctt cccgcttata gcgggagggg
    12541 taggttttgt gaacgtgggc cagcatggga tggaaggcct ggatgggccg gttttccgga
    12601 aggatttcgt cctcgaagaa gaggacatcg ccgcgcatga agttcttttc atcgaagaag
    12661 cggaccacct ttttgattcc cgggttggtg gtcttctcgg ggtcatcgga gacctttaac
    12721 ttgggcttca tctcgccatc ctcgaggatg gccgaaagct tgtacacccc tcccagggcc
    12781 ggacaggagt aagaggtcac catcctcgtc ccgacgcccc aaagatcgat ttccgccccc
    12841 tgcttcttga tggactcgat cagccactca tccaggtcgc tggagccgag aattctggtt
    12901 cgatcgaaac cggcttcgtc gaggagcttg cgcgcttccc ggctgagaaa agtcaggtct
    12961 ccgctgtcca gacggatccc caataggtcc ccgtccttcc tggcgcgaag ctcgttgccg
    13021 actttgatcg cgttggggac gccgctgaga agagtatcgt aggtatctac caggaggacc
    13081 acattcttgg gatagacctt ggcgtaggac tggaaggatt ccagctccga ggggaaggac
    13141 tcgatccagc tatgggcaac cgtcccccgg acgggaattc caaaaacctt tccggccatg
    13201 agattggagg tggcccgggc ccctccgatg aaagccgccc gggaagcgct caggccgccg
    13261 tccggcccct gggcccggcg gactccgaat tcgatcaccg ggtcctcact ggcaacgata
    13321 cagacccggg cagccttggt ggcgatgagc gtctgatagt tgagaatatt cagcagggcg
    13381 gtctcgatga attgcgcttc cggcagggga gcggttaccc ggatgatggg ctcctggggg
    13441 aagaccaagg tgccttcggg aaccgcatag aggtctccgg tgaaacgcag attctgaaaa
    13501 aacgtcagca cttcgggggg gaaaagattc aagcttcgca agtaatcgag gtcttcaggc
    13561 tcaaagtgga ggttttcaat gtattcggtc agctgctcca ggccggctgc gacgcagaaa
    13621 ccgccgtctt ccgggatctt acggaaaaag taatcgaagt tcgctttctg ctggcttttc
    13681 cccaaaaggt agtaagcccc gaccatggtc aattgatata gatcggtcag cagcgtcatg
    13741 ttcagtttca tctgtccatc ctcagttgtt ggttactcga ttctgcttgc tggttgctcg
    13801 ttacaggtta ttggccagtt agctcgttcc tttgctttat attttttaac caggaacaag
    13861 taaccaggaa ggatcaaccg gtcgtccagg atcagatgta gttctccatc agggcatgga
    13921 tttccttttc gatttcctgt tccggtacat cctttccctg ttcctcccga atcgatcgtt
    13981 ggaccttctg gatgatctgg tccacccgca ggagcaggtt ctgatcatcc agatcatcca
    14041 ggttacggag gtaaccaagc cttcggccca cgcgaaactg aaaacgcttt ttcttaggcc
    14101 aggccaggta gcgatcgatg agggccagca tcttttcctt atcctcgggc agccttccct
    14161 ggatttcttc aagaaggttt agaatgtggt cgctcaccac cgtgctgtgg atgccatcca
    14221 aggtttccag gaaaagacga atctcgatta cgatctggtc gtccgaaggc cggacgaatt
    14281 ctcctttttc cactttctgg tacaggggca tgtttttgcg gacgtacagg gtccgcaggc
    14341 ggatgaagtc gggatcgatc tggttcaaaa cccgggcgga ctcgacggcg tgttctcgcc
    14401 accatcgttc tccccccagg ccgggcatga tgtattccga aagagagatg cccgaggcta
    14461 cgacgtttcg gccagcggtg atctgttctt cggccgttac tccctttttg atgtattgca
    14521 ggagaggctc gtacccggtc tccatcccga tatggatgcg ggtaagccct gcctcccgga
    14581 gttctttcag ctccttcaca gattttttgg ccagggtccg cgcccggccg taactggtaa
    14641 tccgttggat ggagggaaat ttttctctga gatagcgcaa aatcgccacg aggtcgccgg
    14701 tcttcctcat gagtgagttg ccatcctgca aaaaaacctg gctcccccca aaatagagcc
    14761 atccggctac ggtctgccaa gccgggttct gggctttggg ctgagaaaga aaatggtgaa
    14821 ggagcgtttg atcaattttt cctgcctgcc ccatcctcca ggagagctgc cagatttggt
    14881 cggcgatctc cttggcctgg tctatgtcct tttgaacttc ttccaaggat cggatggaaa
    14941 aggtttcccc tttgtaggtg tggcaaaatt cacactggtt ccaggggcag ttgcgggtga
    15001 cccggatcag gagactttgg gcttcgctcg gcgggcggat gggccccatt tcaattaggg
    15061 aatccatggc ctcttcctct ttaagtaaaa tctaacatga ttttcgggtt ggatcaatgg
    15121 ccgctgccag ggtgaaatgg ccaatttcca gaggaatgat ctgctccatt cggtctttga
    15181 ttggtttcac atttatcttg attttccccc cgaaactcta tataaacatc tgggtcggaa
    15241 aggaaattga aatccttaag aagccgaaaa ggggaatccg atggctgatg ctctcgatta
    15301 cccgcgaatt cgcagcgtcg atgcctttcc ggcggaggtt tccggacaga aggtcgtctg
    15361 ccttcgggat cctctaggcc tctcggggaa ggtccttttt ttcccctacc caaccttttt
    15421 catcatcagc ctcttcgacg gaagtcattc gctggtggat atccaagccg agttcatgcg
    15481 tcatttcggg gaactcctct accgggagaa gattcaggaa ctcattgccc agcttgacga
    15541 gcatttcctt ctggacagcg agcgcttccg ggatgcggag cagaaaatca tcgatggctt
    15601 tcaaagctct cccctgcggt ccctaaccct gaccgaggac tcttatgggg gaggggcaga
    15661 aaaactcaag gagaccatcg aatcctactt cctggacccg gaagggcccg ggcctccggc
    15721 gaagaaaagg gattcgcgct tcttggtcgg agccatcgcc ccccatatcg actaccggag
    15781 ggggggacct tgttacgcct gggcgcaccg ggcaattctg gaatcctccc ccgcggactt
    15841 gttcgtcatc ctgggaaccg cccattcagc catgaaacaa ccttttgccc tcacccggaa
    15901 gcattttcaa actccctggg gcacggtgga aaccgatcag gaattcatgg acactttgga
    15961 tcgggaggtc tctggggatt tttatcagga tgagtttgcc cacaaggggg aacactccat
    16021 tgaactccaa ctggtctttt taaaagccct ttggccgaag accgacccct ttcggatcgt
    16081 tcccatcctc tgcggttctt ttcacgaggc tattctccag gatgaaagcc ccatggaact
    16141 gccgggggtg gcttccctta tccaagccct gaagaaggcc atggccgggg cgaagaggag
    16201 ggtctgcgct ctagccagtg cagacctggc ccatgtgggc cctcgttttg gagaccccat
    16261 tgtccccgac cgaatttccc tgcagaactt ggccgaggaa gaccggcgcc tgctgggtta
    16321 tgccgaaggg gtcgatggcg aagggttctt cagaattttg gcccgggaga aggaccggag
    16381 aaaagtgtgc ggcctttcgc cgatctatgt tctcctccat ctcttggaag gggcccgggg
    16441 aaaattcctg aaatacagcc aatctctgga ctccacaacc cagtcggtag tcacatttgg
    16501 cagtgtagcc tattattctt gaagggcccc cgcaaccgca gaaattgaac actaggccgc
    16561 gggaaaagac gtaaaaaaca gggtatcctc cggttcatcc cctggggtgg gaggagaacc
    16621 agatggggaa tccccttaca ggaggagaaa gaatgaaaag gaaggtcgtc ttgaaatctc
    16681 cggtttattg ggtcctggga ggcttggccg caggtatttt cctgactggt tttttctggc
    16741 tccgggaacc cacccgagcc tttggcggaa actttgcgcc catggcccaa cagcagttcg
    16801 cccgttttca gatttccggg cgggatgcca attccgcctg ggtgatcgac acggctgcgg
    16861 gggatgtttt tctgattttt tccaacggga aatggaaaga ggtgggaagt atctttgatg
    16921 aaaaaagccg gctcaagaac tgatccgcag attacgaaga ttgcgcagat caccaaaagg
    16981 gctaaggcct gaaaactccg cgatctctgt gcatcctgtg gctcattgct ttttggtttt
    17041 ggttccgtcg cgtcatgctc tctggggttt ggttcttcaa atctcatcga tagaaaggag
    17101 aactgaatgg ccaaggaatg gagactgctc gacctctacg tggacttgaa tgagaactcg
    17161 gccttcttga accccgccat ccagagggcc cggatggaag gcctggtgcc ggatacggtg
    17221 attttctgca gcatcaagaa aatcggaatt tctctgggtt tttatcctga tccggaaaag
    17281 gacataaacc tggaatactg ccggacccat gacatcgggg tgagccggcg tttgcagccg
    17341 tattatgtga acatcggcta tccgggattc atcgtggttt atatcatcgc gggcaaaaat
    17401 tttatgcctc ccacggtggc cgagattttc aagaccgtca tttcccagat ggccgaagaa
    17461 ttcaacggcc ggtggggcac tgcagcccag taccggccgt tcaacgacat ggaagtgggc
    17521 ggaaggaaga tatcctggat gggaggaagc ttttttgggg atgtctggag cggggccagc
    17581 gttctgctgg tcaaaccgtc tcccatcgat aagatcaacc cggccctgaa cattcccccg
    17641 gaaaagttcg ccgataaaga cgccaagact gcgggagaaa gggccgtatc cctggaagaa
    17701 ctggctggcc ggccgatccc cctggaagaa gtcagggaag cgtatcgcca gtctttggaa
    17761 acgaccttcg gggtcaagct ggtcctgggt tgcctcagcg aaatggagat gaaatacgag
    17821 caggaaaatc gggagaagta ttcttcagaa atccttctcg cccggacgga agggaagaaa
    17881 ttcggtcccg tggcaccggg ggtcaaacgg ggggaggcga tctggaaggt tccccggggg
    17941 gggctggtcc gggcggtcgc ccgggtcaaa gaggggacca ttgagaatct gctcttttcc
    18001 gggaatattt actgttcccc tgtgaccgct ctggaagagg ttgaaggatt gctgaaagga
    18061 gttccactgg aggaggatca aattcgggaa agagtaaagg tcatctatgg gaagccaaat
    18121 tatcagctta ccggaaccac accggaggat ttggcgcgcc tggtgctgga ggcggggcga
    18181 aaggcgatct aaaggaaaat gaggaaggga ggaaacgaaa tgagcgagga gagcaagggc
    18241 actatcagcg aggaggagtt tgtgtaccga gcgatcaaaa ggctgcggaa gccgccgtac
    18301 cggggaatcc acagcgtatt tagcgggttc aaccaggcgt tcaaggagca cttcggtaag
    18361 aacccgatcg aaacgaccca gaagatggcc ggggaaggaa agatcgtcac ccggccggtg
    18421 aagggagggg tcatgctcta ccttcccggc gatgcacccc agccgcagaa gggtgtcctg
    18481 cagaagattc tggggccaga ggaagagtcg gcataagatt atcgtccccc agaaggagag
    18541 ggttcgatga accaaccttt ttgggggacc ccattcattt gcttccagga tcgcttaagc
    18601 tgcatgatga ttttgggatc gtcgactacc tgatgaagaa atttgactaa aggagtgacg
    18661 gcatggccaa gaaggaaaat cttcaggcga ggttaaggat ttcgggaaaa cagctggacg
    18721 ccatcaacga tctcttgctg gaccccaaga gccgggtagt aaaggacttt ctggccgtgg
    18781 tggccaagta cggaacccca gaggaaatca acgccaaggc ggccgaggct gggaagctag
    18841 aaaacctcat ggatcggctg gagaaggaga aatcccccta tttagcagat gtgaaatgga
    18901 tgatagctca gagggacgca aaagctttcg tttcggttgc cgaatttcga aagtcgcttc
    18961 tgggggagaa ggccgcgcgg atgaagttca aggaaaaatt cgcggtgacc ctggagatca
    19021 gcgcagctca gtatttcccc tgggtgatcg aggaggccag gcaagccatc gccaaaagag
    19081 agctcatgcc tggacggttc atccgggttc ggaagatgaa agaacaggaa ggagacaacg
    19141 gggacattct agcctttgcc gcggccatgc aggttatggg agctagtttt gtggaaacat
    19201 tggacacgaa ggggaccgac gggtccaatg ttcacctggg ggggccggat acgatcaccg
    19261 ggtatttcgg ggggatcggg cagcccaacg aatatcctct aaaatgggtg gatgaattcc
    19321 tctactactt tacgaactat gggatccgcc aggttttgaa cattaacccg ggaaccgtct
    19381 ttctgggata catgctcggg aagctgggga tccagaacga gttcaagatt tcggtgttca
    19441 tgggaaacga taatccctat gccgctctgt ggaccctgat cgggggcaag ctcttctccg
    19501 ctaaagacgg ctcctgcccg ctcatcggct ttaacttgag caactcggtc aacaaccaga
    19561 ccatcgagcg ggtagccgaa gttcgcaaca agctgggctt cgaggacatc gttcgaatcg
    19621 aacatcacat caccgaaacc tggaagagca tcgtccgcca gccttacaac cggagggatg
    19681 aattggttcc gctcgccggc cacgtggcca acatcgccgc caagcacgaa ggaagcgaga
    19741 tcgaaaccga aaaaggacga cagcatcctt ccgatatctt cgactatttc cgggagaaaa
    19801 aggaaatcga agcctccggg gatatgcccg ctttgctcca gaactacctg gacaaacacg
    19861 attcggttaa cctcacggcc cgggctttga ccgagaaagg gctgagcttc aaggcggcat
    19921 cgaatttaca tcaccggaaa taattttctg attggaccat cgttttgttt tctcccttga
    19981 ccgaggccct tatgccggga agagggctgc ctaaccaaaa cgggaaagac tttctttgac
    20041 gaaattgaag gcgggatatt tttactccgg acgctcacgg ggttccaccc aagcaccgaa
    20101 gcggtaattt ccagtgcttc aatttaacga aaaggaggat tctatggaag cggaaaaggg
    20161 ccgtgcagag cgttttgcct tagcggcgga aaaatatgtc cccgatgcca ttacctccgc
    20221 cgtgatcatg gtgatcattc tttttctcta cgcctggtcc atcggggtgc ctgcggacaa
    20281 gaccacggat gctttctacc gggggttctg gatgctgctg gccttcacca tgcagatgac
    20341 cctgatcatc accctgagcg ccgtcttcgg gcaatcgccc atctttcgga aggccatcat
    20401 ggccctggcc aaggtgccca actcgccctt ccaggtggtc atccttgctg ccctggccac
    20461 gggggtgctg gggtattttt actggggatt ggcaatcacc ttggggccgg tcattgccgt
    20521 ctatttctgc ggggaagcgg agcgcaaggg cattaaagtg gacttcatgc taaccctagc
    20581 cgtcgtcttt gctgcccatt ccgtgtggca gtacggttta tcctccagcg cgcccttgct
    20641 ttccgccacc ccggggcact ttctggagaa attgactggc aaaatgcctt tgagcagcac
    20701 catctggacg cccgcggcga tcttccaaat cgtcgccttc atggtcattt tactcatcgt
    20761 cgcccggtac ctcgtgccca gagtgcccaa gaccatctcc gaatatcccg aggcctttaa
    20821 gcttatcgag gcg
//
LOCUS       DASPRR010000514        44387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1574101, whole genome shotgun sequence.
ACCESSION   DASPRR010000514 DASPRR010000000
VERSION     DASPRR010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 44387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44387)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44387
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574101"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VEL68_18925"
     CDS             complement(<1..1296)
                     /locus_tag="VEL68_18925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ38106.1"
                     /translation="MKTRIRLTDWPPNTLSVCMIVKNEEKNVEECLETVKDFADQVVV
                     VDTGSTDRTVEIAEKYGIAVIRSDWRGDFSYSRNISLDHATSQWVLWLDADDRVPLSE
                     AEKFKRLKTAPPDRAFYLKVRNVRQGGFGEQWYQLRLFPNHPEIRFERKVHEQVGFAV
                     KKLKMPVFRVDVRIDHIGYDNPEVKRKKALRNREILLAEFPRYRRDPAYVSSIGDSYF
                     LTEDFEAAIQWYQRVLEIPDGERKQPEIFRQMPTSIALSYERLGDWGNAHQWIEKEIQ
                     ASPQKIDSLFLAAGLREKMGDLAGAAALYERITQIPANYTSYAADLDGLKAKSLISLG
                     NIYTKRERFDEAEKAFRRCLERYPQVLNSYSELGELLLKRGKLNEAADLFIGSIKRYR
                     GADSKAYFGLAKALALMGQLEEAARVLDEAKTLYTSYSQI"
     gene            1574..2002
                     /locus_tag="VEL68_18930"
     CDS             1574..2002
                     /locus_tag="VEL68_18930"
                     /inference="COORDINATES: protein motif:HMM:NF041863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVU0524 family FlgM-associated protein"
                     /protein_id="HXZ38107.1"
                     /translation="MVIHPFYTQQVLKAYNVQLSMRPRISKGQTNSPASKDVIAISPE
                     SRKKSMVAKISQEIVNNLAKNSPRNETAEEALNQLSQTYGQLFEVEEKAGEGLAFRVV
                     SEKRGDPPRSLTKEENEKIKQKLLDITQTIVYRDLSKGGI"
     gene            2003..2317
                     /gene="flgM"
                     /locus_tag="VEL68_18935"
     CDS             2003..2317
                     /gene="flgM"
                     /locus_tag="VEL68_18935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HXZ38108.1"
                     /translation="MKIQDMNNDGQSIRQITQGMKPPQSEKNPPASSVTSPDAGSDRV
                     ELSPQSRDMKKIHEILATTPEMRTEKVAELKKAIADGSYRVNTEDIADKMLKEMVFES
                     NR"
     gene            complement(2358..3248)
                     /gene="flgL"
                     /locus_tag="VEL68_18940"
     CDS             complement(2358..3248)
                     /gene="flgL"
                     /locus_tag="VEL68_18940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HXZ38109.1"
                     /translation="MRVATPTLYRGIQQNILRLFSDLNKIQEKVSSGKNFNRPSDDPV
                     AMVNSLGLSNTLERIGQYQKNMDTAKGWLDINETVLDQVSNLAASAQNIALQLSGGNQ
                     TAEVRTMAVSQIDHLLDEAIGLGNTQLNGKYIFGGYQTATPPFARSVSGGTETVNYQG
                     DGNDLKIQIDPNQTITAGKNGATVFMDSNLFSSLIALKQAIANNDQTAIQQESTNLQA
                     ATDYLNAQVSDVGIRQDHLKIQGEIFSQVNLDLQNQLGDLENVDNNKAIVEMQQKQTA
                     YNVALETAAQISKVSLLDYL"
     gene            complement(3259..4710)
                     /gene="flgK"
                     /locus_tag="VEL68_18945"
     CDS             complement(3259..4710)
                     /gene="flgK"
                     /locus_tag="VEL68_18945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HXZ38110.1"
                     /translation="MSGINAIMNIAAGALQADQIAMDVASHNIANVNTPGYTRQSAVF
                     ESGSPVSMNQLRIGTGVQIASVAQAFDPYTTRTIQQNTSSLNEYQTEASVLSQLESLF
                     NETRDSSLSNAMSDFWNAWQGVANNPGGTVERTALLEKAKILTQKINSLSNDLTGTKK
                     DMDNNLQMGIEKVNELTKQIADLNGQIVSAEAAQTPANDLRDQRNSLLEELSSWVGNV
                     SVEQGNGSVTVFTQGGILLVDGNRNWKFSTQGDSIYYNNVPSDVSKSLTGGKIGGWLD
                     IRDEIVPQYQANLDELAGTLIQQVNTLHTAGYTLSGGTGTYFFQDFLTSPAVPNATDF
                     KGAAAFISLSSDVLGHPENIAAGGVSGAPGDNENALNIAAIQTDGSLSIRKWTISNRG
                     GSTSSSVETTSMDDYYRTLVGEIGITTEAANENQSFVQSKLDDLRDLRDSVSGVNLDE
                     EMTQLLKLQRAYEASSKIISIADEMLQSLLQSV"
     gene            complement(4713..5225)
                     /gene="flgN"
                     /locus_tag="VEL68_18950"
     CDS             complement(4713..5225)
                     /gene="flgN"
                     /locus_tag="VEL68_18950"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HXZ38111.1"
                     /translation="MTPLREGLLEEFCQRMEEEILHYHLLGEELKKESGFLRQGSVDL
                     LTESLHSVEMHVEEIRRVHGSIARDIEKMVSTLRPEEKEKTLGTLLILLPAKDAQRIK
                     GYQKTLASLKKRVSQVNTRNKTFVQGSLAYWRELFSLLTQPLVESPVYIQNGKTQSST
                     LLPISLNRKV"
     gene            complement(5346..5768)
                     /locus_tag="VEL68_18955"
     CDS             complement(5346..5768)
                     /locus_tag="VEL68_18955"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HXZ38112.1"
                     /translation="MESMPINKMGKAPEAGSQGNDPGSRKIDREKLKKACSDFEALFL
                     ARMLKVMRQSVPSSGLWGNGPGKEIYDSLMDQELSKKMAQRGGIGLGKKIYNQVLKRE
                     EKSEPLSAEGQLRGAGRKEEVNERAGRSSAAGLSDDRR"
     gene            complement(5781..6977)
                     /locus_tag="VEL68_18960"
     CDS             complement(5781..6977)
                     /locus_tag="VEL68_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HXZ38113.1"
                     /translation="MKKRSVLIGLAVITGLMMSLPSFAWGARLKDIAFFKGVRSNQLA
                     GYGLVVGLNGTGDNTTSVDFTIRSIANMLERMGIKIPPDRILVTRLKNVAAVMVTATL
                     PPFGGVGNRIDAHVSSMGDATNLGGGTLLMTPLLGPDRQVYAVGQGAIVTGGFSVSGA
                     SGSSITKNHPTAGLISKGAVIEKEVPVTLDGKKELALTLFHPDFTTAEKVKEVINLSM
                     GGAYSRCVDSATIKIAVPDSHQDRVAEWMASLERLEVTPDAVAKVILNEKTGTVVLGE
                     KVRISTVAVAHGNLSIQIKERYNVSQPLPFAPGSPPGTPSIQIPPREGTGPIMTPGGA
                     TVVTPESSLAVKEEGKPMIILPSGADLGEVVKALNAIGATPRDLISILQSIKAAGALQ
                     ADLEVY"
     gene            complement(7004..7690)
                     /locus_tag="VEL68_18965"
     CDS             complement(7004..7690)
                     /locus_tag="VEL68_18965"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HXZ38114.1"
                     /translation="MKKLSSLPAALILVSIWGTGCLIPQASSKSEYSLPLAAAQHYSS
                     PYAPLSEGSLWVSARGGNLFADVKARNVGDIVTINVVESATASNNAATKTTRASNLEA
                     SWSGVLSKITGDWVGSDQKVDFANNFDGKGDTTRSSQLSTYITAQVFQVLPNGYLAIQ
                     GNRQVRVNNENQVINVQGVIRTEDISASNIILSTYISDARIELIGQGAVSDKQRPGWL
                     ARILDWVWPF"
     gene            complement(7707..8690)
                     /gene="flgA"
                     /locus_tag="VEL68_18970"
     CDS             complement(7707..8690)
                     /gene="flgA"
                     /locus_tag="VEL68_18970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HXZ38115.1"
                     /translation="MSRSRFLRELFFLGLFLISACWTGEASPSPLSVNFRTQASVQKE
                     VVYLEDIASIQGSSPSLVEKVGRTKIQASPLPGDVLILSRASLSAALQRNGISSLVAQ
                     SQIPDKIEVTRPGRSMERAELSQILEDHFQRALGDEKKSVKVREIQGIDPFMVPPGVL
                     SWEVRAPAQFYQGGNIPVFLILWAEGEKVREVRAQARVEVFAEVVVAKNSLRRHQIVE
                     EKDVQLVNRNITQQAADVITDVGEVLGKRLTLSINTQEVLRKSMVEVPPLVKKGDRVT
                     LLVENDHFKITCMGEVKEEGRAGDRIKVTNISSNREVYGQVLDGHTLLVDF"
     gene            complement(8702..9484)
                     /gene="flgG"
                     /locus_tag="VEL68_18975"
     CDS             complement(8702..9484)
                     /gene="flgG"
                     /locus_tag="VEL68_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HXZ38116.1"
                     /translation="MMRGLWTAATGMNCQQTNLNTVANNLANVNTTGFKKSRVNFQDL
                     MYQNLRMPGASTSTGGKLPAGIQVGMGAKVVAVEKVHLQGDYTQTKNQLDLAIEGKGF
                     FKLLSNGKEVYTRAGSFKQDKDGYVCDTYGNRLQPEFAVPPKTLTVTIEPGGKIVASG
                     SDGKELGSAQIQLYNFPNPAGLMSLGQNLMAPSDASGDTIPGNPGTDDFGTLLQGSLE
                     MSNVDVVEEMVNMITTQRAYEINSKVIQTGDEMLQLASNLKR"
     gene            complement(9518..10225)
                     /gene="flgF"
                     /locus_tag="VEL68_18980"
     CDS             complement(9518..10225)
                     /gene="flgF"
                     /locus_tag="VEL68_18980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HXZ38117.1"
                     /translation="MGIFQNLAVTGLFRQERRHELIANNLSNAQTAGFKKDVPVFHKI
                     FSEAFSSNPLGEFETSVTSFQQGELQATQNPFDLAIEGEGFFKVKTPQGIRYTRSGHF
                     RLNREGTLVQDSGYPVWGRTGEINLRGNQFSVDKDGTVNVDGSIRDKIALVTFPDLQG
                     LRKEGQALFKLEGEQEEKEASQSQLHQGALELSNINIMEEMIRMIDALRSFQACYKIV
                     QVQDDMDAKAVNELAKV"
     gene            complement(10478..10774)
                     /locus_tag="VEL68_18985"
     CDS             complement(10478..10774)
                     /locus_tag="VEL68_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HXZ38118.1"
                     /translation="MRNQEKGEKLPKAVALRYIPKSDRAPKVTAKGSGRVAEKIIALA
                     KEHGIPIQEDPALIQVLSQLDFYEEIPPSVYGVVAEILAFIYSLNQKYGSMTQG"
     gene            complement(10781..11782)
                     /locus_tag="VEL68_18990"
     CDS             complement(10781..11782)
                     /locus_tag="VEL68_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38119.1"
                     /translation="MEPHRFLLQLGDQKFWVESRVPLPPSGDFPLRVEEIEPKVILKL
                     LSPQAPGELKIDSLLKRYLSADVPLENLADRLSLLVKAGPQGMPSSVQRALEEVRSLL
                     RSLPDLSALRQDTLRRLVENSGFFWENKVKNWMEGARGEPLSQVLQEDLKGLGMKLLA
                     QLQGVGREGMQEGDPSRVENLKQDLESFLQKVEFYQVLNSRSPERTDGLFLFFPFWLG
                     SHLQLVELNLSFPKRESAQGGEDGFSLLFLLNLPSWGKVRIEVQMREKTLFCRFLVED
                     RQVAESIHFGFSTLRAHLEKLGFASHLQASVEGEENMKETVVQELGKEAPSLLSIII"
     gene            complement(11924..12400)
                     /locus_tag="VEL68_18995"
     CDS             complement(11924..12400)
                     /locus_tag="VEL68_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38120.1"
                     /translation="MSLNDTWGLLVVEGTILASFLTLFFFLRGTPKGISSKRGKALAG
                     GRKLEGNLEKINQLLKESESLSGDLAQTLVEKRELVKSLVESLDGKIQNLSQLLEKIE
                     EKIPAFPPKASAQEGKGQILEMATAGCGVADIAKRLGLSQEEVQLILDLRKVATHG"
     gene            complement(12423..12566)
                     /locus_tag="VEL68_19000"
     CDS             complement(12423..12566)
                     /locus_tag="VEL68_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38121.1"
                     /translation="MDKDSRRRSKGKKRERGRPAVEESALEDREEGQNEKPASGKVVD
                     ILV"
     gene            complement(12673..13056)
                     /locus_tag="VEL68_19005"
     CDS             complement(12673..13056)
                     /locus_tag="VEL68_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ38122.1"
                     /translation="MSESNMKFLVVDDFATMRRIVKNILRQIGHTNILEAENGQMALD
                     ILRREKVDFIVSDWNMPQMPGIELLKAVRSQEEWKDLPFLMVTAEGQKENVLEAVKFK
                     VSNYIVKPFTPETFIEKVNKILEKK"
     gene            complement(13132..13923)
                     /locus_tag="VEL68_19010"
     CDS             complement(13132..13923)
                     /locus_tag="VEL68_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HXZ38123.1"
                     /translation="MKSRAFNHKVYSTSPKTSGPPPPEANNREEMILKYTPLIKYIAH
                     RLAMRLPPHVSVEDLMSAGVIGLMDALNKFDPEKKVQFKTYAEFRIKGAMLDELRSLD
                     WVSRSVRQKAAQLEKACQTLEKKRGRSVEDEEVAEEMGLSLESYYDLVNEVNGVFLLD
                     IENVRHKIPKLPEEDLINLLIDDQEKQPLHLLGLEELKKVLAEAIEELSPKEKTVISL
                     YYYEELTLKEIGEVMGFTESRICQIHAKAILKLKTKVRSYMRDFT"
     gene            complement(13920..14858)
                     /locus_tag="VEL68_19015"
     CDS             complement(13920..14858)
                     /locus_tag="VEL68_19015"
                     /inference="COORDINATES: protein motif:HMM:NF012369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HXZ38124.1"
                     /translation="MDQAEGLRTLMDSQKERSEPLGKPPKKSKEAYTARWPRVISVGS
                     GKGGVGKTNVVANLAFAFTRLDRKVLVFDADLGLANIDILLGLTPQYNIQHLLNREKG
                     VFEILTRGPGGMAVLPASSGVLELVDLNESQKLFLLNELDLVAGTVDILLIDTAAGIS
                     SNVLYFNLAAEESIVVVTPEPTSITDAYALIKVLATKYKKERFTLLINSAQNGQEAKE
                     TFVKISRAVDRFLGGISVDYLGFVPFDDKIPQAVKKQRPVLEAFPQALSSRSFMEMAR
                     ELIERPPTPRTQGQVQFFWQHLFQCQQRTEQDGRAD"
     gene            complement(14873..15913)
                     /locus_tag="VEL68_19020"
     CDS             complement(14873..15913)
                     /locus_tag="VEL68_19020"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38125.1"
                     /translation="MQIKRFEAPDMQQALRRVKEALGPEAIILSTKSLKKLNGRTRAF
                     SPMAVEVVAAIDHAPAHGARPARKVAPLWLPPTAKKKECFSPSEDPFLERMLSIGLAP
                     DFVHRLGEELKIWLKESRTERNSETYRNYLQWKFMDEVEVTGADLGIGKVWSFIGPTG
                     VGKTTTLAKLAAHFSLELKKKVTLVTIDTYRIGAIDQLSTYSRVLGLPLEIASGREEL
                     GEILQRNRQQDLVLIDTAGRNPYQLEQLEELKDILSVDRRMENHLVLSATTKDYDLAQ
                     IGERFSLLPIRSYIFTKIDETEEFTSLFNQLLRFRRPLSYLTNGQRVPEDIELATKGR
                     VAHLVLNRIQWN"
     gene            complement(15903..18008)
                     /gene="flhA"
                     /locus_tag="VEL68_19025"
     CDS             complement(15903..18008)
                     /gene="flhA"
                     /locus_tag="VEL68_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HXZ38126.1"
                     /translation="MTEKVIQSPVLSRESFRLERSEVCMAGAVIAILLVMIIPLPTLL
                     LDILLSCSFTISILVLLTSLYITHPLEFSTFPSVLLLTTLFRLSLNIASTRLILLYGN
                     EGTSAAGVVIKSFGQFVVGGNYVVGFIIFIILVIINFVVITKGSGRIAEVAARFTLDA
                     MPGKQMSIDADLNAGLIDEQEARRRRAQIAREADFYGAMDGASKFVRGEAIAGILVIL
                     VNIGGGFVIGVFQQDLPLLTAAQTYTILTIGDALVSQIPALIISTAAGIIVSRAASEF
                     SIGKELSRQFRLQPQAIGLSAAILLVMGLIPGLPHIPFITLSLMVGGVAYLTLQENRR
                     QAQRKEEETKKAAPPPSAEGAESLLQLDRLELEVGYGLIPLVDEEQHGDLLSRIRSLR
                     RQFASDLGIIIPPLRIRDNLQLHPGEYRVLIKGNEVARAEMMMGYLMALNPGEVKKTI
                     EGIPTKEPVFGLPALWVPEKRKEEAHFSGYTVVDISTIMATHLTEILKGHADELLGRQ
                     DVQKLLDRLAQNYPKAVEELTPNNLALGVVQRVLQNLLRERVSIRDLLTIVETLADYG
                     PVTKDPEVLTEYVRQKLSRSIVKQFETPEGTLPVIALDPRIEDLLREKLVRGEQGSYL
                     ALEPGVAQKILTSIHQTLDRVAHLNYQPVILCSPGVRRHLKKLLDRFLPQVAVLSHSE
                     LTSQTKIQSLGTVSVAHAD"
     gene            complement(18015..19082)
                     /gene="flhB"
                     /locus_tag="VEL68_19030"
     CDS             complement(18015..19082)
                     /gene="flhB"
                     /locus_tag="VEL68_19030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HXZ38127.1"
                     /translation="MADESFGERTEKATGKRRAEARKKGQVARSREIPSVAVLLVALS
                     VIYVLSTYLLQRLSWVMVQSFQKIGTFSLSPDNILAFRGEVVWALFQILTPILLAVLA
                     MGVLSNYVQIGSLFTLEVIQPKLSRVDPIQGFRRLFSKESLVEVVKSIFKFLIIGWVA
                     YSTVQKELPEILTLSDQEVGGVFRFVGSVSARIFFKTVLVMVALAVLDYLFQRWSYEK
                     SLRMTKREVQEEFKQTEGDPLVKSRIRSIQREMARRRMMAEVPKADVVITNPTHLAVA
                     LGYEKDEMAAPKVLAKGAGVIAEKIKETARAHQIPLVENKALAQILYKTVELGRVIPS
                     TLYQVVADILAHVYRMKNRTL"
     gene            complement(19085..19876)
                     /gene="fliR"
                     /locus_tag="VEL68_19035"
     CDS             complement(19085..19876)
                     /gene="fliR"
                     /locus_tag="VEL68_19035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HXZ38128.1"
                     /translation="MPAVLVNWSLLQFQALVLILMRVSPILFMMPVLNSRSIPSLVKA
                     GLTLTVGLILLPLVKVDPRLLPSEPIQFGLLMAAELMIGLVLALSVNIVFAGIQFSGE
                     LVGFQMGLSMASVVDPQSEVDTPLLSQFLDLVSLIVFLTVDGHHWFFRALFQSFLLLA
                     PGEIHLQEGLYRHFLGLLGSLFVVAIKIAAPVMAVLIFTQIGLGILAKAVPQINILIT
                     SFPLTLGIGFLFLALSIELFLPYFRNLFQETGRALVFTLLPLMQR"
     gene            complement(19888..20157)
                     /gene="fliQ"
                     /locus_tag="VEL68_19040"
     CDS             complement(19888..20157)
                     /gene="fliQ"
                     /locus_tag="VEL68_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HXZ38129.1"
                     /translation="MNPDLVGQIAREALETTLYLSLPILGIGLIVGLLVSLFQAVTQI
                     QEATLVFVPKIVVVFVALLVLGPWMMRKMMFYTEQLIVNLPNFVK"
     gene            complement(20301..21065)
                     /gene="fliP"
                     /locus_tag="VEL68_19045"
     CDS             complement(20301..21065)
                     /gene="fliP"
                     /locus_tag="VEL68_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514551.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HXZ38130.1"
                     /translation="MKGKLLPWLLLLSVAGVIGMTYSSASAQAWPIPTVKIGVGEAKG
                     PGEVSVLIQILILLTVLSLAPAILVMMTSFTRLIVVFSFLRHALGTNQMPPNQLLIGL
                     ALFLTFFIMTPVWQTIDEKALTPYKEKKISQAQALEEAVKPVRKFMFKQTREKDLALF
                     IQMAKMSRPKNQEEIPTTVLLPAFMISELKTAFQIGCILYIPFLIVDMVVSSVLLSMG
                     MMMLPPVMISLPFKVLLFVLVDGWNLLVGSLVKSFY"
     gene            complement(21055..21432)
                     /locus_tag="VEL68_19050"
     CDS             complement(21055..21432)
                     /locus_tag="VEL68_19050"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HXZ38131.1"
                     /translation="MLWATLKMVLALGVVIALLVLLVRLFKRWQRVQGILGPDNGIRV
                     LTTKPIAPQKYVSLVEIGGEVLALGVTPQQITFLTKVENKEMIQKSLTRPGFKPEAIP
                     WLKSLALFPRKERGEMAEMGNER"
     gene            complement(21440..21787)
                     /gene="fliN"
                     /locus_tag="VEL68_19055"
     CDS             complement(21440..21787)
                     /gene="fliN"
                     /locus_tag="VEL68_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HXZ38132.1"
                     /translation="MELENLGNTKEPGIKPGAEPKAEGSPKEVPNLDFILDLPLEVSV
                     ELGRARMLVSELLQLGQGSVIELNKLAGEPVEIFVNQKLIARGEVVVVNDKFGIKMTD
                     ILSPMERVKQLGS"
     gene            complement(21803..22777)
                     /gene="fliM"
                     /locus_tag="VEL68_19060"
     CDS             complement(21803..22777)
                     /gene="fliM"
                     /locus_tag="VEL68_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HXZ38133.1"
                     /translation="MDKILNQDEIDALLKGIEGGKVEVTPEPKEESKVAAYDFTNQDR
                     IIGGRMPTLEVINDSFARLLRTPLSVTLRKNVDIVPGKSLLTKYGELTKTLPLPCSIQ
                     VFKMNPLRGHALLILDPKLVFSFVDIFLGGTGRTNFRIEGRDFTHIESKLIQKVVNII
                     FVELEKAWNSVHPLSFQHVRSEVNPQFASIVPPGELVLTISFNLEVEQITGFITLCIP
                     YFSLEPIKGKLYSGYQSEHMELDQNWIARLSEELKFTEVEVVVEFAKAVVTSEKLLGL
                     KAGDILPLGKEVSEPLTAKIQGVPKFLGRAGVYGSSKAFQIEGTLKPA"
     gene            complement(22785..23135)
                     /locus_tag="VEL68_19065"
     CDS             complement(22785..23135)
                     /locus_tag="VEL68_19065"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HXZ38134.1"
                     /translation="MFFKGKPSPPPATAAANGHIYKMEPFLVNLADPGQPRYLKVTLH
                     IESNQEKPSEEYEKRQPQLRDAILTILSSRQYREIMTSEGKAALREEIKGKVNQLLVS
                     LKAQNIFFTEFVIQ"
     gene            complement(23575..24837)
                     /locus_tag="VEL68_19070"
     CDS             complement(23575..24837)
                     /locus_tag="VEL68_19070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HXZ38135.1"
                     /translation="MSITSSFYSALSGMDSNSVAMQVISDNISNVNTVGFKGSSVQFE
                     DLLGMSLEGISGVTRVGVGAKVSSMACSFTQGTLETTGVGTDLAINGKGFFIVKNTTT
                     DEDFYTRAGSFHLDKDGYFVNANGLRVQGYLYDPTGTNLIESLSDIVVDQTNMIAPAA
                     TSNVQMVLNLNSAETPTTFNIADPGGTSHYSTALPVYDSLGQTHTVTVYFTQTAAQTW
                     SWNALIDGADVSGGTAGTPQLFGTGTIAFDSSGALTTAMPVALYTGAITYADGVAASA
                     VSADFTGTTQYGAPSAIQSLLQNGYGAGTISGISITNEGNIVGNYTNGQVKNIARLAL
                     ADFPSLYGLERAGSLLFRSMPDSGDPLINKAGEGGVGNVSASMLEESNVDLAGEFIKM
                     IITQRAYQADSKIITTTDEMLAQLINIK"
     gene            complement(24861..25520)
                     /locus_tag="VEL68_19075"
     CDS             complement(24861..25520)
                     /locus_tag="VEL68_19075"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HXZ38136.1"
                     /translation="MSVTAVGTSSSSNSQGVEKASKNTLTQEDFLKLFTTQLQFQNPL
                     EPLDNFQMATQMAQFNTVDALTKMNQTLTQLATEQTSMNNLQVASLIGKKVKTQGNGL
                     AIQQGTVGEGFYQLANPGKVFVQVFNSQGTLVRQIDAGFKDASLQKIGWDGKNQAGVT
                     LPDGTYTFKVLAVDTQGKAVSVTTYQVGTVDGVSLDNGSVALQVNGGKVQFSDILSIM
                     N"
     gene            complement(25541..26965)
                     /locus_tag="VEL68_19080"
     CDS             complement(25541..26965)
                     /locus_tag="VEL68_19080"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HXZ38137.1"
                     /translation="MSPSGDKSPENPVPAIYSFISSISSGVQEAGPTLQESPIPLDSS
                     ISQTSLEEPLQRPLAGNLEMALEKGRKTAHPVDPSTPAISLEEVLLKESTGKKDMPTE
                     SGKKIVPFSDSPNTPVSLEEVLLRESTGKMDMPADSGKKIVPFSDSPIAPVSWEESLR
                     KESAEKMELSAGGEKNFIRPLIQEESNGSERRPVQESKDFFPSFEPFLKMEANPRTGE
                     GQVLKKQGPLFGAAKEAAGPEIPQNLQERGPLVGNQAYILDSPDKESGTPFSADAGKG
                     QEKFLWVGPDIRPLAAREVQNPPSFLAALTDPLNRHVEYAGEIKEASPPPSERPDPQA
                     VFQQVGQRVLWLVRNNEERIRITLEPPELGQVFLEIDRHKEHIKTTLWTDNPTTKANL
                     ETSQGEIQRIIESEGFKLEKFDVFVQQDPGWFQGRKEDTGNPDSWKARMPVEEKASSA
                     NSMESIPGRTPAPDLASRYLDLLV"
     gene            complement(28044..28499)
                     /gene="fliJ"
                     /locus_tag="VEL68_19085"
     CDS             complement(28044..28499)
                     /gene="fliJ"
                     /locus_tag="VEL68_19085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HXZ38138.1"
                     /translation="MPFHFSLQPLLNWKKSLEEQSQMRLAGMMTRLKAKEEEIEKLTL
                     QRLACEQKLKEKSEQGMPAGEYVLFKQFGEESRKELISKEDEKRRILREMDQERGKLL
                     ALTKEKKTLERLKEKQLKEFLSRLQKAEQKRADETTTLKYQPRPRGDIS"
     gene            complement(28581..29906)
                     /locus_tag="VEL68_19090"
     CDS             complement(28581..29906)
                     /locus_tag="VEL68_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HXZ38139.1"
                     /translation="MTPVPDSLWASLHQAIQKGETIRRYGRVNQLIGLVIEATGPMAA
                     IGDMCQILSEGKEGAIPAEVVGFRNQKILLMPLGEVRGVEPGCRIVSLGFPAQAPVGQ
                     ALLGRVLDAMGNPTDHGGPISKEVEAPLYAPPSSAVLRKRIHEPIDVGVKAINALLTI
                     GKGQRMAIFSGSGVGKSTLLGMMARHTTAEVSVIALIGERGREVREFIEKDLGEEGLK
                     RSVVVVATSDQHPLLRMRGAYLATALAEYFRDQGRDVLLMMDSITRFAMSSREVGLAI
                     GEPPTAKGYTPSVLSQLPKLLERAGTTPDRGSITGIYTVLVEGDDLMDPVADAIRSIA
                     DGHIVLSRNLANQGHYPAIDVLESISRVMNDIVPPEQTDARTKLVSVMATYRRSEDLI
                     NIGAYVTGTNPQIDYAIKKMPEINAFLRQGVAEKVGLAESLARLKAIVG"
     gene            complement(29903..30652)
                     /locus_tag="VEL68_19095"
     CDS             complement(29903..30652)
                     /locus_tag="VEL68_19095"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HXZ38140.1"
                     /translation="MSKVFIAQFSSPVEPSREFMAEGLGPEAGQPRVVPVEWPCLQTS
                     GENLQWSQGMKEKEEKILRQARERALEIEKDAYEKGFVQGEKDGAELGRKRWETVFDS
                     FRQLADELGRIQQDLPRRYEREMVLLVFAIAKRILRLDLPLREETLLKTLQAAFQHVV
                     DPRNVIIHLHPKDHQHLITHPEGLPWDPKGGESHNIRFISDPTVTRGGCFLETSFGEI
                     DATLETQLEQILSQVGSSFDPPLTPSDLSRP"
     gene            complement(30645..31688)
                     /gene="fliG"
                     /locus_tag="VEL68_19100"
     CDS             complement(30645..31688)
                     /gene="fliG"
                     /locus_tag="VEL68_19100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HXZ38141.1"
                     /translation="MKEKTPNQAPAQEKISGPQKAAVLLFSLGEDLAAEIVKNLDENE
                     LRKLGGSMSRVFSLAPEVFNSVFDEFHQLAASPQSLALSPGQRSAFIKNVFSKAIRGE
                     KAQALIEEIQQKERLNLFQKVRKLDARTLASFIHNEHPQTIAVVLAHLDPGHAAAILE
                     ELPERVQNEVLYRIAQLEKVPPAILAEIDQILQQEISMFENVEGGQVGGVRSAAEILN
                     QMDPSAETQVLQAMEEQQQGLADEIRRWMFVFEDFINVDDRSIMAILKEVEADSLKVA
                     LKTASDELKERIFKNMSERAVTMMKEDLEVMGPVRLKDVEVAQQAIIKIGKKLEAEGK
                     VILTGKGKEDVFV"
     gene            complement(31669..33324)
                     /gene="fliF"
                     /locus_tag="VEL68_19105"
     CDS             complement(31669..33324)
                     /gene="fliF"
                     /locus_tag="VEL68_19105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HXZ38142.1"
                     /translation="MANPLFNPISQWIGSPAWKRNLSIALVLVFSLIGFGSLIYWNSK
                     PEYQVLFSNLSTEDAGEMVNKLKEKKIPFQLSSNGTSILVAREQVYDTRLTLAAEGLP
                     KGGGVGFEVFDRSNLGATDFVQKLNYQRALQGELSRTIRQIKEVEQARVHIVTPKESL
                     FLEDQKKPSASVLIKTRSGMKLDAGQVEGIVHLVASAVEGLDSGNITMVDTSGKILFK
                     RNETTLLGQMTSHQLDYQRNIEEGLKKKAQGMLEEVLGFNKAIARVSADIDFQQVDIT
                     EERFDPNSVVRSEQKNSEKSSMISNARAPAGGKPENLPAVKTPEEKGNPAPEEKGKAA
                     PETKARTAEPVPVQSNHSERQNEVRNYEISRVNKRIKSPVGVLKKISAAVIVDGTYKE
                     VADAKGKKGREYQARSPEEMKSIEAMVKKAIGYDEGRGDQVEVINMSFSWSVAEEESK
                     AVAGEWWKEYILISYKPLVSLILAVLFIFFVVRPLLRRKASLPQEEAAYLPKTSPLPA
                     LPPDLSPQPKTLDLKTQTVQLIQENPSKAVGIIKEWINEGKDA"
     gene            complement(33344..33643)
                     /gene="fliE"
                     /locus_tag="VEL68_19110"
     CDS             complement(33344..33643)
                     /gene="fliE"
                     /locus_tag="VEL68_19110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HXZ38143.1"
                     /translation="MDDLRIRKISSVPLEKAGADRSEVLDPISDFKKILGRSIGEVNG
                     LLQEADQSVQEMVAGKIDIHQAMTALEQANLSFRLMVQVRNKMITAYEEIMRMQF"
     gene            complement(33664..34104)
                     /gene="flgC"
                     /locus_tag="VEL68_19115"
     CDS             complement(33664..34104)
                     /gene="flgC"
                     /locus_tag="VEL68_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HXZ38144.1"
                     /translation="MNLFLSMQISASGLSSQRTVLRVIAENMANVQTTRTEEGGPYLR
                     KRAILSPTPVPSSFPELLFSRMRPEALGVQVTDIVEDPYGLKSVFDPSHPDADETGLV
                     LLPNVNLMEEMVALMGASRVYEANVAAFNAAKTMALRALEIGSK"
     gene            complement(34108..34494)
                     /gene="flgB"
                     /locus_tag="VEL68_19120"
     CDS             complement(34108..34494)
                     /gene="flgB"
                     /locus_tag="VEL68_19120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HXZ38145.1"
                     /translation="MFDKTMSLIQRSMDIRASRHRVLSSNVANIETPEYVSRDLPFQK
                     VFEGCLENSSILPLGKTHPAHLPATEGPLSQDFSSHIEMDPGPGGVDIDQQMSRLAEN
                     NLMFQAGVQALIKKFEALKVGIEESR"
     gene            complement(34583..35950)
                     /locus_tag="VEL68_19125"
     CDS             complement(34583..35950)
                     /locus_tag="VEL68_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ38146.1"
                     /translation="MNRRPILIVEDEPELGRVLLESLSQNGYSAHLAESAEKALNLIG
                     QKAFDLIVSDIRLPHMNGLQLLDHVRGYSHPTPVILMTGFGSVQNAVEAMRKGAFDYL
                     LKPFSLETLEQGIENALRQSFSGQMGEAESPCPPSRVPIITQDRKMKEVLALCGKIAP
                     SKATVLIQGESGTGKELLARYIHEQSQRNQGPFVAVNCASLPETLLESELFGHEKGAF
                     TGAMARKIGKFELSHRGTILLDEISEMNTFLQAKILRVLQENEVDRIGGNRPIPIDLR
                     VIATTNRDLESWVKKGELREDLYYRLKVIEVILPPLRERPGDIEVLTYHYLRKFADEY
                     ARPGRSISEAALTWMKEQEWRGNVRELKNVIERAVLISSRKTLEIQDFREDTGGEVEK
                     NRGAEGAESVSFCLRHMEQRMISKALEKTQGNRTHAAKMLGISIRTLRNKLNEYKKEL
                     PAGLS"
     gene            complement(36296..37681)
                     /locus_tag="VEL68_19130"
     CDS             complement(36296..37681)
                     /locus_tag="VEL68_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ38147.1"
                     /translation="MSNEKILVIDDSPEILTLFSEYLRAEGYEVDTSADGASGIEMVE
                     KKSYDLIISDLKMPGMDGMKVLEFAMEHSPDSICIILTGYGTVKNAVEAIKMGAFDYL
                     TKPVKMDEMLVTLRRALEYRSLKVENINLKNQLKKKYKFENIIGDHEKMQKVFEIVEK
                     VADTDSTILILGESGTGKELIAKAIHYNSYRRERPFVPVNCAAIPSELLESELFGHEK
                     GAFTNAIRTRIGRFELANGGTVFLDEIGDMNPLLQSKLLRVLQEKQFERVGGIKTIKT
                     DIRIIAATHQDLKQAVQQKKFREDLYYRLNVIPLRVPPLRERRTDIPLLAHYFLDHFN
                     RSKKKKIRAIEDGAMEGLIGYEWPGNVRELENTIERVVILVDHDVIAAPDLPEKFHSL
                     PQPEPPQLLEIPEEGISLDTAVNEFEKNLILQALMKTGWVKNKAARLLNLNRTTLIEK
                     IKRQNLQRPDN"
     gene            complement(37888..38217)
                     /locus_tag="VEL68_19135"
     CDS             complement(37888..38217)
                     /locus_tag="VEL68_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38148.1"
                     /translation="MRSHSAINRRPAFLIVLLGFLLSFFSGGERTACAQPGKVPPGTE
                     PERISPEPKEGIGAKEVPREDGGKDKIYYSIITPEEERKAKQEEKEKEERSWDILKNI
                     IIDNHRR"
     gene            complement(38288..38767)
                     /locus_tag="VEL68_19140"
     CDS             complement(38288..38767)
                     /locus_tag="VEL68_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38149.1"
                     /translation="MDVKEIGGPPLAFGKKGKREEMEGADFQKALQEAHSHLKESPSP
                     STSTGGAGGAEWLEPVTFQLLPSNLAVGAPSPQSQGTEVTERTLNLLERYQAALSDPR
                     VSLKEVYPLVQSLRQEVQELDRWSDQLPPSDPLRKMMGEIGILSNVEIEKFNRGDYV"
     gene            complement(39076..39558)
                     /locus_tag="VEL68_19145"
     CDS             complement(39076..39558)
                     /locus_tag="VEL68_19145"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HXZ38150.1"
                     /translation="MSKRGIKRESLKSGGLLFLGMTAAFLFLAPWMEMALFSDPGIGR
                     AWAGEKREEHPPSAMDVGPMIKLSSLVVNLNEASCRHYIRTTIVLEVAQKEWVDEVQK
                     RIPLLTDMIIVTLGDKKLTDLKGPQGREELRKELLVKAEKTLASPRIRQIYFDEFLLQ
                     "
     gene            complement(39662..40711)
                     /locus_tag="VEL68_19150"
     CDS             complement(39662..40711)
                     /locus_tag="VEL68_19150"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXZ38151.1"
                     /translation="MERQRIKVLIVDDSAIVRKVFTEALSKEPDIEVVGSAPDPFVAR
                     DKIVQLKPDVITLDIEMPRMDGLTFLKKLMQYYPLPVIVVSSLTPQGSKMAVEALESG
                     AIEVLAKADSAYSVGDMSLQLIEKIRLAARANIHRTRSKPAPPPLMRGAENGALSQTT
                     QKVIAIGASTGGTEAIKEILMQMPPNIPGIVIVQHMPAYFTQSFAERLDSLCGIRVRE
                     AKDGDGVMNGQALIAPGNFHMVLKRSGARYFVNITEGPTVHHQRPAVDVLFNSVAQHA
                     GANAVGVILTGMGSDGAEGMLRMKEAGARTIAQDEASCVVFGMPKEAIRLGAVEWIGS
                     LEKIPQEIVRRAQST"
     gene            complement(40770..41261)
                     /locus_tag="VEL68_19155"
     CDS             complement(40770..41261)
                     /locus_tag="VEL68_19155"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HXZ38152.1"
                     /translation="MFEEIRQSAVGAVSKVFEEMFFIFPEEVGESCDTRGGEAQGGGE
                     ENPSAASIRAEIEFDGVPSGKIILILPDGLARKMTGNLMGMNEAEVGDPEILDMIGEL
                     NNMISGNLFFLLKRKGEYRLTKTKAERVSETQTQTEPQDGGMVIHFSADGERVKLMIQ
                     LEP"
     gene            complement(41254..41640)
                     /locus_tag="VEL68_19160"
     CDS             complement(41254..41640)
                     /locus_tag="VEL68_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ38153.1"
                     /translation="MGYNVLIVDDSQTMRKVIRKSVDLSGLPLGECWEAGNGVEALDL
                     LGSHPIDVILTDFNMPGMNGLEMIRELQKNEKHCRIPVVLITTHSQDWVIQEALSLGI
                     KGFIRKPFHPEAIRDLLSQVMEEKSV"
     gene            complement(41689..42537)
                     /locus_tag="VEL68_19165"
     CDS             complement(41689..42537)
                     /locus_tag="VEL68_19165"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXZ38154.1"
                     /translation="MNKIDLILQSITHMPSFPAVVHRVLELVENPKTSSKDVVEVIQY
                     DQATTANVLRVCNSAYFGPRKPVHSLNEALVRIGFNQLMEIVLSKGTASFFTRACQGY
                     HLEAGELRRHSVASAILSQILAQQIQKETSPLQFTTALLHDIGKLALSEFVRESFEEI
                     RTLVRERKISFLEAEKEILGIDHAELGARIAEQWEFAPGIVAGIRFHHTPFQAPDFQD
                     LVSMVHLCDVVALLTGCGGGADGLSYHGYKEIMQRFGLQEKHLERVISELDDRLKEAE
                     MLLNSP"
     gene            complement(42534..43013)
                     /locus_tag="VEL68_19170"
     CDS             complement(42534..43013)
                     /locus_tag="VEL68_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HXZ38155.1"
                     /translation="MNLVVGMGDMKVSDDPQAMIVTFALGSCIGITIYDPLVKAGGLL
                     HFMLPESSTNPEKALRNPFMFADTGIPRLFQEIYKIGGDKRRLRVKIAGGAQLLDESG
                     FFNIGKRNYAALRKILWTGNLLIHAEDVGGQVNRTVRLDLSTGKVSVKTSGEGEKEI"
     gene            complement(43010..43864)
                     /locus_tag="VEL68_19175"
     CDS             complement(43010..43864)
                     /locus_tag="VEL68_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HXZ38156.1"
                     /translation="MNPGGYPWSNPELTDGEFEKISRLVYEACGINLTEGKKELLKAR
                     LGKVIRAGSFGSFREYYEHVVQDSSGQELVHLLDSVSTNFTSFFREPQHFEYLKSEFL
                     PEVLGDKGRNGRKLRFWSAGCSSGEEPYSIIISLLESLESPRGWDLQVLSTDLSSKVL
                     KVAAAGIYPEGRIQTISPDLVKKYFLRGENRWKGYVRVKDSLKKYLVFRRLNLMEPFS
                     FAEPFDGIFCRNVMIYFDKPTQASLLNRFFQSLGRGGVLFIGHSESLAGIEHPFRYVQ
                     PATYRKEL"
     gene            complement(43895..>44387)
                     /locus_tag="VEL68_19180"
     CDS             complement(43895..>44387)
                     /locus_tag="VEL68_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HXZ38157.1"
                     /translation="KFNERGKKPMSMKAELINPFLEATLSVLKTMASAEAVPGKPYIK
                     KGSAACGDISGVVGITGQAEGSLCLTFSREAILQVMTSMLGEEQKEITEEVKDAVGEL
                     TNMISGDSRRRLQEKGYTFHGAIPSVISGPGHEVKHITKGPVLSIPFSLNGGNFVVEV
                     CFK"
BASE COUNT         9358 a        12385 c        11719 g        10925 t
ORIGIN      
        1 tatctggcta taagaggtat acaatgtctt tgcttcgtca agaacccttg cggcttcttc
       61 taattggccc atcaaggcta aggctttggc taatccaaaa tacgccttcg agtcggctcc
      121 acgatatctc tttatcgagc cgatgaaaag atcggccgct tcattcagct ttccccgctt
      181 caagaggagc tcccccaact cggaataaga atttagaacc tggggatacc tttctaaaca
      241 ccttcggaag gctttttccg cctcgtcgaa tctttccctc ttagtgtaaa tatttcccag
      301 agaaatcaag gacttggcct tgagcccgtc caagtcggct gcatacgagg tatagttggc
      361 agggatttgg gtaatccgct cataaagggc tgctgcccct gcgaggtcgc ccattttctc
      421 ccgtaatccc gcggccaaaa aaaggctgtc aatcttctgc ggtgaagcct gaatctcctt
      481 ctcgatccat tgatgggcat tcccccaatc ccccagccgt tcatagctca aggcgatgga
      541 agtaggcatc tgccggaaaa tttccggctg tttgcgctct ccatctggga tctccagcac
      601 cctttggtac cattgaatgg ccgcttcgaa atcttccgtc aggaagtacg aatctccgat
      661 ggaactaaca tacgcaggat ctcgccggta tcggggaaat tcagccagga ggatttcgcg
      721 attgcgcaag gcttttttac gcttgacctc ggggttgtca tatccgatat gatctatccg
      781 aacatccacc cggaacaccg gcatttttaa ttttttgacc gcgaagccga cctgctcatg
      841 gacctttcgt tcaaagcgaa tttctggatg attgggaaac aggcgcagtt ggtaccattg
      901 ttctccaaac cctccctgcc gcacatttcg aaccttcaag taaaaagccc ggtcaggagg
      961 ggctgttttc aacctcttaa acttctccgc ctcggaaaga ggtacgcggt catcggcatc
     1021 cagccaaaga acccactgcg aagtggcatg atccagggat atatttcgtg agtaactaaa
     1081 atcaccgcgc caatcagaac ggatgactgc gatcccatat ttctcggcta tttccaccgt
     1141 gcggtcggtg gaccctgtgt cgaccaccac gacctggtcc gcgaaatcct ttaccgtttc
     1201 caaacattcc tcaacattct tttcctcgtt tttgacgatc atacaaacag aaagggtatt
     1261 tggaggccaa tcggttagcc gtattcttgt cttcatttgt actggttttt cgtcatattt
     1321 ttgacatctg aggccttctc tattttttca gtgcacggaa catgccagat ttggcctctt
     1381 tttctcttgt tcccgtggta attgggaggg cctaatttta tccaaacgaa tccatcagat
     1441 aggcggtagg tttccatctt ttcctcccag caaataaatt caagaattct gtcaattaaa
     1501 atttcttccc cccagcggaa aaaaactcaa gcttttgcaa gaggttgacg acaagactta
     1561 taggtaaagg actatggtta tacacccgtt ttacacacag caagtattga aggcttataa
     1621 cgtgcaacta tcaatgaggc cacgaatatc gaaaggccag acgaatagtc ctgcttcgaa
     1681 agacgtgatt gcgatctcac cggaaagcag aaaaaaatcc atggttgcca agatttcaca
     1741 ggaaatcgtc aataatttgg ccaaaaattc accccggaac gaaacggctg aagaggccct
     1801 gaatcagctc agtcagacat acggccagct ttttgaggtg gaggaaaaag ccggagaagg
     1861 attggccttt agggtcgttt cagaaaagag gggggaccct cctcggtccc taacgaagga
     1921 agaaaacgaa aaaatcaaac agaaactgct tgacatcact caaactattg tttatcgtga
     1981 cttatctaaa gggggaattt gaatgaaaat tcaagatatg aataacgatg ggcagagcat
     2041 ccggcaaatc acgcagggaa tgaagccccc ccaatcggaa aaaaaccctc cggcttcgag
     2101 tgttaccagc ccggatgcag gttcggatag agtggaactc tctccccaat cccgggatat
     2161 gaagaaaatt catgaaatct tagccactac tccggaaatg cgcacagaaa aagtggcgga
     2221 gttgaaaaaa gccatcgcgg atggaagtta tagggtaaac accgaggata ttgctgataa
     2281 gatgctcaag gaaatggtct tcgagtccaa ccgttgattc ttctcctgtt tatgcagggt
     2341 ctcgctctcc tcgcccatca caggtagtcc aataagctga ccttcgaaat ttgggcggcc
     2401 gtctccaagg caacgttata ggccgtctgc ttttgttgca tctccacaat ggctttgttg
     2461 ttatccacat tttcgagatc acccaattgg ttctgcaggt cgagattgac ctggctgaag
     2521 atttccccct ggattttcag gtgatcctgc cgaatgccca cgtccgagac ctgggcgttg
     2581 agataatccg tcgccgcctg aaggttcgtc gactcctgct gaatggctgt ttgatcgttg
     2641 ttcgcaatcg cctgtttcag ggcgatcaag gaggaaaaca gattggagtc cataaatact
     2701 gtagctccat tctttccggc cgtaatcgtt tggttggggt cgatttgaat cttcaaatcg
     2761 ttcccgtctc cctgataatt aaccgtttca gtcccgccac tgacgctgcg ggcaaaggga
     2821 ggagtggcgg tctgatatcc tccaaagatg tatttcccgt ttaactgggt atttcccagg
     2881 ccaatcgcct catccagaag atggtcaatc tgcgacactg ccatagtccg gacctcggcg
     2941 gtctggtttc cgccgcttag ctggagggcg atattctgag cgctggcggc caggttggaa
     3001 acctggtcga gaacggtctc attgatgtcc agccatcctt ttgcagtatc catatttttt
     3061 tggtactgtc cgatccgttc cagcgtgttg ctgaggccca gcgaattgac catggccacc
     3121 gggtcgtcgg aggggcggtt gaaatttttc cccgaggaaa ccttctcttg aattttattc
     3181 aaatcagaga aaaggcgcaa aatgttctgc tggattccgc gatataaggt tggagttgcg
     3241 acacgcatcg tttttctcct ataccgattg cagcagggat tggagcatct catccgcgat
     3301 gctgattatt tttgagctgg cctcataagc ccgctggagc ttcagcaatt gggtcatctc
     3361 ctcgtccaga ttgacgccgg acacggaatc ccgcaagtcc cgaaggtcat ccagcttact
     3421 ctgaacgaaa ctttggtttt cgttagctgc ctcagtggta attcctatct ccccgaccag
     3481 agttcggtaa taatcatcca tggacgtggt ttctacgctg ctcgaagtgc ttcctccccg
     3541 gttgctaatc gtccatttcc gaatcgatag gctcccgtcg gtctggatgg cggcgatgtt
     3601 cagggcattt tcgttatccc ccggcgcccc ggaaacccct cctgcggcga tattctcagg
     3661 atgccccaga acatcggagc tcagggaaat aaaggcggct gcgcccttga agtccgttgc
     3721 gttgggaacc gccggagagg ttaagaaatc ctggaagaag tacgttcctg tgcctccgga
     3781 aagggtgtat cccgcagtat gaagggtgtt gacttgctga atgagggtcc cggccaattc
     3841 atccaaatta gcctgatatt ggggaacaat ctcatcccga atatccagcc agcccccgat
     3901 ctttcctccg gtcaagcttt tgcttacatc acttggaaca ttgttgtagt agatcgaatc
     3961 cccttgggta ctgaatttcc aattcctatt tccatcgacc agcagaattc ccccttgagt
     4021 aaagacggtc accgagccat tcccctgctc tacagaaaca ttgcctaccc aactggatag
     4081 ctcctccagg agactatttc tttgatctct caaatcattc gccggggttt gggcagcttc
     4141 agcggacaca atctggccat tcaggtctgc gatctgttta gttagctcat tgaccttctc
     4201 aattcccatc tggagattgt tgtccatatc cttcttggtc ccggtcagat cgttgctcag
     4261 agaattaatt ttttgcgtca gaatttttgc tttttccagc agggccgtcc gctctacagt
     4321 tccccccggg ttattggcca caccctgcca ggcattccag aaatcgctca tagcattact
     4381 tagggaggaa tctcgcgtct cattgaagag ggactccaat tgggacaaaa ccgacgcttc
     4441 ggtttgatat tcattcaagg aggatgtatt ttgctgaatc gtccgggttg tataaggatc
     4501 gaaagcttgg gctactgaag ctatttgtac ccccgtccca attctgagct gattcattga
     4561 aacgggagat cccgattcga ataccgcact ctggcgtgta taccccggag tattgacatt
     4621 ggcaatatta tggctcgcca catccatcgc aatttggtcg gcctggaggg cacctgcggc
     4681 aatattcata atggcattga tcccggacat aactacacct ttcggtttaa agaaataggt
     4741 agaagagtcg aggattgcgt cttcccattc tggatataaa ccggcgattc caccaggggc
     4801 tgggttagaa gagagaaaag ctctctccag tatgccaggg atccctggac gaaggtttta
     4861 ttccgggtat tgacctggga gacccttttc tttagactgg ccagagtttt ctggtagcct
     4921 ttgatccgct gcgcatcctt tgccggcaag aggatcagga gggttccaag ggttttctct
     4981 ttttcttccg gtcgtaaggt acttaccatc ttttcaatgt ctctagcgat ggacccgtgg
     5041 accctgcgga tctcctcaac atgcatctca acagagtgga gggattcggt aaggaggtcc
     5101 acggacccct ggcgcaggaa tcccgactct tttttcagct cttctcccaa aagatggtag
     5161 tggaggatct cctcctccat ccgctgacag aactcttcca gaagcccctc tcgcaaaggg
     5221 gtcacggttt cttctcctct tcctgctctt tttcctcgcc cgtttccagc agacctgggg
     5281 ggaacaggct caggcattcc gttcgcttcc tgatttcctg aaggaaggag tgaaagtggc
     5341 aatgttcatc ttcgatcatc tgacagacca gctgcagatg atcttccagc tctttcattg
     5401 acttcctctt tccttcccgc ccccctgagc tggccttctg cggaaagggg ctcgctcttt
     5461 tcttccctct taaggacctg gttatagatt ttctttccca ggccgatccc gcccctttgg
     5521 gccatttttt tgctcaactc ctggtccatg aggctgtcgt aaatctcttt accagggcca
     5581 ttcccccaga gcccgctgga aggaacggat tgtcgcatga ctttcagcat ccgggccagg
     5641 aacagggcct caaagtctga gcaggccttt ttcagtttct cccgatcgat cttgcggctt
     5701 cccgggtcgt ttccctggga gcctgcttcc ggggctttac ccatcttgtt tatcggcatg
     5761 ctctccattt tcttgaattc ctagtagacc tccaggtcgg cctgcagggc cccggcagcc
     5821 ttgatgctct ggaggatgga gatcaggtcc cggggggttg cgccgatggc attcagggcc
     5881 ttgacgacct ctcccaggtc cgccccgctg ggtaggatga tcatgggctt tccttcctcc
     5941 ttgaccgcca aactcgattc gggagtgacc accgtggctc ctccgggggt catgatgggt
     6001 ccggttcctt cccgcggagg aatttggatg gaaggggtcc cggggggaga cccgggggca
     6061 aagggcaagg gttgggatac gttgtaccgt tccttaatct gtatgctcag gttgccgtgg
     6121 gccacggcaa cggtggagat ccggaccttt tctcccagaa ccaccgttcc agtcttttca
     6181 ttcaggatga ccttggccac tgcatcgggg gtgacttcca acctttccag ggacgccatc
     6241 cattctgcca cccggtcttg atgggagtcg gggacggcga tcttgatggt ggctgaatcc
     6301 acacatcgag agtaggcgcc gcccatagag aggttgatca cctcctttac cttttccgcc
     6361 gtggtgaaat caggatggaa gagggtcagg gccaactcct tcttcccgtc taaagtgacg
     6421 gggacctctt tttcgatcac ggcccctttg gagatgagtc cggctgtagg atggtttttc
     6481 gtaatgctgc ttcccgaggc cccggaaacg gaaaaccctc cggtcacgat cgcgccttgt
     6541 cctacggcgt acacctgacg gtcaggaccc aggagagggg tcatgagcag ggttccccca
     6601 cccaggttgg tggcatcccc catggaggaa acatgggcat cgatccggtt gccaacccct
     6661 ccaaaaggag ggagggtggc ggtgaccatg accgcggcta cgtttttgag gcgggttacc
     6721 aggattcgat ccggggggat tttgattccc atcctctcca acatgttggc aatggaacgg
     6781 atggtgaagt ccacactggt ggtgttgtcg ccggttccat tgaggcccac gacgagtccg
     6841 tatcccgcca gttggttact ccgaaccccc ttgaagaagg cgatatcctt cagccgggct
     6901 ccccaggcaa acgaaggaag ggacatcatc agcccggtga tgacagccaa accaatcagg
     6961 accgatctct ttttcatggc ggcgctcctg cccgaaacct ttcctagaac ggccataccc
     7021 aatccagaat cctcgccagc cagcccggac gctgcttgtc gctgaccgct ccctggccga
     7081 tcagttcgat cctggcatca gaaatataag tggaaaggat gatgttgctg gcgctgatgt
     7141 cttcggtccg gatcacccct tgcacattga tgacctggtt ctcgttgttc acccggactt
     7201 ggcggtttcc ctggatggcc agatacccgt tgggcaaaac ctgaaaaacc tgcgcggtga
     7261 tgtaagtgct caattgagag cttcgggtcg tatccccctt gccgtcaaaa ttattggcga
     7321 agtctacttt ctggtcgctt cctacccagt cgcccgttat cttggacagg accccgctcc
     7381 agctggcttc caggttcgag gcacgggtag tcttggtcgc ggcgttattg gaggctgtgg
     7441 ccgattccac tacgttgatg gtcacaatat ctccaacatt tcgggctttc acatcggcaa
     7501 acagattgcc cccccttgcg gaaacccaca gagagccctc ggacaggggt gcatagggag
     7561 aagaataatg ctgggctgct gcaaggggta gagagtattc gctcttcgag gaagcctggg
     7621 ggatcaaaca tcctgttccc cagattgata ccagaatcag ggctgcggga agggaagaaa
     7681 gctttttcat caagcttggc tcctttttaa aaatccacca acaacgtgtg gccgtccaag
     7741 acctgtccat agacttctct gttgctggag atattggtca ccttgattcg atctccagcc
     7801 cggccttctt ctttcacttc gcccatgcag gtaatcttga aatggtcatt ctccaccaac
     7861 aaggtcaccc gatccccttt tttgaccaaa ggaggaacct ccaccatgga tttgcgcagg
     7921 acctcctggg tgttgattga aagggttaac cttttcccaa gcacttctcc cacgtccgtg
     7981 attacatccg cggcttgctg ggtgatgttt cggttcacaa gctggacatc cttttcttcc
     8041 acaatctggt gccttcggag ggaatttttg gcaaccacca cctcggcaaa gacctccacc
     8101 cgcgcctggg cgcggacctc ccgaaccttc tctccctcgg cccaaaggat caggaatacg
     8161 gggatattgc ccccctggta aaactgggcc ggagctctga cctcccagga gaggaccccg
     8221 gggggaacca taaacgggtc gattccctgt atctccctaa ctttcacgct ctttttctca
     8281 tcccccaggg ctctctggaa gtggtcttcc agaatctggc ttaactcggc tcgctccatg
     8341 gatcggcctg ggcgagtgac ttcaatctta tccggaatct ggctttgggc caccagggaa
     8401 gaaattccgt ttctttgcag ggctgcggaa agggaggccc gggagaggat taaaacgtca
     8461 cccggaaggg gcgaggcttg aattttcgtc ctgcccactt tttccaccag cgaaggggaa
     8521 gagccttgaa tggaggcgat atcctcaagg tacaccactt ccttctggac tgaagcttgg
     8581 gtcctaaaat tcaccgaaag gggagaagga gacgcctctc ccgtccagca ggcggaaatg
     8641 agaaaaagac ccaggaaaaa caactctctg agaaatctgc tccggctcat gggcttggtt
     8701 cctatctctt caaattggac gccaattgga gcatttcgtc cccggtctgg atgaccttgg
     8761 agttgatctc gtaagccctc tgggtggtaa tcatattgac catttcttct acaacatcca
     8821 cattggacat ttccagggag ccctggagga gggtgccgaa atcatctgtg ccggggttcc
     8881 ccgggatggt gtctccggag gcatcgctgg gggccatgag attctggccc aaggacatca
     8941 agccggccgg gttgggaaaa ttgtacaact gaatctgagc gctccccagc tctttcccgt
     9001 ccgatccgga ggccacgatt ttccccccgg gttcgatagt gacggtaagg gtcttcggag
     9061 ggaccgcaaa ttccggctga aggcggttgc cgtaggtatc gcacacgtac ccatccttgt
     9121 cctgcttgaa agacccagcg cgggtgtata cttctttgcc gttgctcaag agcttgaaga
     9181 acccttttcc ctcaatggcc aggtccagct gattcttggt ctgggtataa tccccctgga
     9241 ggtggacctt ttccacggcc acgaccttgg cccccatgcc cacctggatt ccggcgggca
     9301 actttccgcc cgtggaagtg gaagcgccgg gcatgcgcag gttctggtac atgaggtcct
     9361 ggaaattgac ccggcttttc ttgaacccgg tggtattcac gttggccagg ttgttggcca
     9421 ccgtattgag gttggtctgc tggcaattca tccccgtggc tgctgtccaa agacctcgca
     9481 tcatcttcga tctcctcctt caggttcctt gggatggtta aactttggcc agttcgttga
     9541 ccgccttggc gtccatgtcg tcctggacct gcacgatctt gtagcaagcc tgaaagctac
     9601 gcaaggcgtc gatcatccgg atcatctctt ccatgatatt gatattggag agctccagtg
     9661 ctccctgatg gagctggctc tgggaagctt ccttttcttc ctgctcgcct tccagtttaa
     9721 agagagcctg tccttctttt ctcaatcctt gcaggtcggg gaaggtcact agggcgatct
     9781 tgtcccgaat ggacccgtcc acattgacgg ttccatcctt gtccacggaa aattggttcc
     9841 cccgcaggtt gatctccccc gtccttcccc ataccggata tccactatcc tggaccaggg
     9901 ttccctcccg attcagacgg aaatggccgc tacgagtgta gcggattccc tggggagtct
     9961 tgaccttgaa aaatccttcc ccctcgatgg ccaggtcgaa ggggttctga gtggcctgca
    10021 gctccccctg ctgaaaggag gtgaccgagg tttcaaactc acccagcgga tttgaggaga
    10081 aggcctcgga aaagatctta tggaagacag gcacgtcctt cttgaatcct gccgtctggg
    10141 cgttactcag gttgttggcg ataagctcgt gccttctctc ctgtctaaag agcccggtga
    10201 ccgccaagtt ctgaaatatt cccacttcga tcctccttca gattctgtct tcaaaaaagc
    10261 aaatagtatg ccactgcggc aaggaaagct gtttctcccc tttctcattt ctgtcccctt
    10321 ggaacatttt tttattagta aaataagcac cttatgacta tctttcggac aggggacggc
    10381 ggacttctgg aaagagattt cctttcccgg aaaacctctt gtttccccat gagaccaagg
    10441 aaaaaaatac cctaaaggaa gggaatttct tgccccctta cccttgggtc atggagccgt
    10501 atttctggtt gagggaataa atgaaggcca agatctcggc gacgacccca taaaccgacg
    10561 gagggatttc ctcatagaaa tccagctggg aaaggacctg gattagggcc gggtcttcct
    10621 ggatgggaat gccgtgctcc ttggccaagg cgatgatctt ttcggccacc cggcccgaac
    10681 ccttggcggt caccttggga gcacggtccg atttggggat gtaccgaagg gctaccgctt
    10741 tggggagttt ctcccctttc tcctgatttc tcatattgac tcagatgata atgctgagaa
    10801 gagaaggagc ttctttcccc agctcctgca caacagtttc tttcatgttc tcttcaccct
    10861 ccaccgatgc ctggaggtgg gaggcgaatc ccaacttctc caaatgagcc cgcagggtcg
    10921 aaaacccaaa gtggatggac tcggccactt gcctatcctc caccagaaac cggcagaaga
    10981 gagtcttctc cctcatctgg acttcgatcc taaccttgcc ccaggaagga aggttcagca
    11041 aaaagagaag tgagaacccg tcttccccgc cctgcgccga ctcccttttg gggaaagaaa
    11101 ggtttaattc cactagttgc aggtgagacc ccagccagaa gggaaaaaag agaaaaagtc
    11161 catccgtccg ttcagggctt cgcgagttca ggacctggta gaattcaact ttctggagaa
    11221 aggactccag gtcctgcttc aggttctcca cccgggaagg gtccccttct tgcattccct
    11281 cccttcccac cccttgcaat tgagccagga gcttcattcc caaccccttc aggtcttcct
    11341 ggagaacctg agatagaggt tcccctctgg ccccttccat ccaattcttc actttgttct
    11401 cccagaaaaa acccgaattc tctaccagcc tccgcagagt gtcttgacga agcgcagaga
    11461 gatcggggag ggatcgcagg agagatcgga cttcctccaa ggccctctga accgatgagg
    11521 gcattccctg gggtcctgct ttgaccagaa gggaaagccg gtcggccagg ttctccaggg
    11581 gcacatcagc actcaggtat cttttcagga gggaatcaat cttcagctct ccgggggcct
    11641 gcggggacag gagtttcaga atcaccttag gctcgatctc ctccactcgt agcggaaagt
    11701 ctccagaagg gggcagggga acacggcttt ccacccagaa tttctgatcc cccagttgga
    11761 gcagaaaccg gtgcggctcc atctgcccga gaattctccc ttgaagaatt tcacccgggc
    11821 ggaagaaagg tacattcagg ttaagcgggg tttcggccgg aaggggggaa ggtggaaagg
    11881 aagagagcag tttgatctcg ttcaaggggt gaaagcgagc cttttatccg tgggtggcaa
    11941 cttttctcag gtccaagatc aactggacct cttcctggga aagccccagt cgtttagcga
    12001 tatctgccac cccacagcca gcagtggcca tttccaggat ttgacctttt ccctcctggg
    12061 cgctggcctt cggcgggaaa gctggaatct tttcctcgat cttctccaaa agctggctca
    12121 aattctgaat cttcccgtct agggattcga ccaaactctt aaccagttcc cgcttctcca
    12181 ctagggtttg ggcaaggtcc ccagagaggg actctgattc cttcagcaac tggtttatct
    12241 tctccagatt gccttcaagc tttctccccc cagccagggc tttgcccctc ttcgaggaga
    12301 ttcccttcgg agtccccctt aagaaaaaga ataaggtcag aaaggaggct agaatggttc
    12361 cctctaccac taataatccc catgtgtcat tcaagctcat ccttgttcct ttgctttccg
    12421 ccctagacca gaatgtccac cactttgccc gatgccggct tttcgttctg gccctcttct
    12481 cggtcttcca gggccgattc ctcgacagcc ggcctgcccc gttccctctt ctttcccttg
    12541 gacctgcggc gggaatcttt gtccacgggc acctctttag agtttcgggt ctgacccgaa
    12601 gggtttagcc gactcaactg accaaagagg ggagactcat cccccatagg aatttttccc
    12661 gtcgcccgaa gcttacttct tctccaggat tttgttgact ttctcgatga acgtctcggg
    12721 agtaaatggc ttgacgatgt agttgctcac tttgaatttc accgcttcca ggacgttttc
    12781 cttctgtcct tccgcagtta ccatcaagaa gggcaggtcc ttccattctt cctggctgcg
    12841 tactgccttc aggagttcga ttcccggcat ctggggcata ttccagtcgg aaacgatgaa
    12901 atcgaccttc tcccggcgta gaatatccag ggccatctgt ccgttctcgg cctctaaaat
    12961 gttggtgtgg ccgatctggc gcaggatatt cttgacgatc cgccgcatgg tggcaaaatc
    13021 atccacgacc aagaacttca tatttgattc gctcatttct ttctccctgg ctgatggggg
    13081 ttgcaggact ttcgtttttc ctccggaagg gaagtttcag gaccttccgg atcaagtgaa
    13141 atccctcata taacttcgga ccttggtctt cagcttcaga attgccttcg catggatctg
    13201 gcagatccgg gattcggtaa accccatgac ttccccgatc tccttcaaag tgagttcctc
    13261 gtaataatag agggaaatca ccgtcttttc cttgggggaa agttcctcga tagcctcggc
    13321 cagcactttc tttaactcct ccagtccgag aagatggagg ggttgtttct cctggtcatc
    13381 gatgagcagg ttgatcaggt cctcctcggg tagcttgggg attttatggc gcacattctc
    13441 gatatccagg aggaagactc cgttaacttc gttcaccaag tcatagtagc tttccaggga
    13501 aaggcccatt tcttccgcaa cctcctcatc ttccaccgac ctccctcttt tcttctccaa
    13561 ggtctggcag gctttctcca gttgggccgc cttctgcctc accgagcggg aaacccagtc
    13621 cagggagcgc agttcgtcca gcatggctcc tttgatccgg aactcggcgt acgtcttgaa
    13681 ctgcaccttc ttctccggat cgaacttatt cagagcatcc attaagccga tgacccctgc
    13741 actcatcaaa tcttccaccg aaacatgagg aggcagacgc atggccagtc gatgggctat
    13801 gtacttgatc aggggagtat acttcaggat catctcctcc cggttgtttg cctccggagg
    13861 gggggggcct gaagtcttcg gactcgtcga atagaccttg tgattgaacg ctctggattt
    13921 caatcggctc tcccatcctg ttcggtccgt tgctgacatt gaaacagatg ctgccagaag
    13981 aattggacct ggccttgtgt tctgggggtt gggggcctct cgatgagctc tcgggccatc
    14041 tccatgaaag aacggctgga gagggcctgg ggaaaggcct ccaggaccgg ccgctgcttc
    14101 tttaccgcct gggggatttt gtcatcgaag ggcacgaagc ccagataatc aaccgatatg
    14161 cctcccagga aacgatccac ggccctactg atcttgacga aagtctcctt ggcctcctga
    14221 ccgttctggg ccgaattgat cagcagggta aaccgttctt tcttgtattt ggtggccaaa
    14281 actttaatca gcgcataggc atcggtgatg gaggtcggtt ccggagtcac taccacgatc
    14341 gactcttccg ccgccaggtt aaagtaaagt acattggaag agatcccggc cgccgtgtca
    14401 atgagcagga tgtctacagt tccggccaca aggtccaatt cattcagcag gaatagtttt
    14461 tggctctcgt tcaggtccac cagttctaga accccggagc tcgccgggag aaccgccatt
    14521 ccccccggtc cccgggtgag gatttcgaaa acccccttct cccggtttag aaggtgctga
    14581 atgttgtact gaggggtcag ccccaggaga atatcgatgt tggccagacc caggtctgcg
    14641 tcgaagacca ggaccttccg gtccagacgg gtaaaggcaa aggccaagtt ggccaccaca
    14701 ttggtcttcc caacccctcc cttgccgctt cccacggaga tcacccgcgg ccagcgcgcg
    14761 gtgtatgcct ctttactctt cttcgggggt ttgcccaaag gttcacttct ttccttttgg
    14821 ctatccatga gggtccgcaa tccctctgct tgatccattc ccgtagacct cgtcagttcc
    14881 attgaatccg gttcaaaacc aaatgggcta cccggccctt cgtggccagt tcgatgtcct
    14941 caggaactct ctgaccattg gtcaggtagg acaaaggccg tctaaaacgc agcagctggt
    15001 tgaagaggga ggtgaactct tcggtctcat cgattttggt gaagatgtaa ctccgaatgg
    15061 gaagcaggct gaagcgctcc ccaatctggg ccaagtcata atccttggtg gtggcactga
    15121 ggaccaggtg gttctccatc cggcggtcaa cggacaggat gtccttcaat tcctccaact
    15181 gctccagctg atagggattc ctaccggccg tgtcgatgag caccaggtcc tgctggcggt
    15241 ttctctggag aatctctccc aactcttccc gtccggaggc gatctccaat ggcaggccta
    15301 gaacccggga ataagtgctc agctggtcga tggccccgat gcgataggtg tcgatggtga
    15361 ccagggtcac cttctttttc agctccaggc tgaagtgggc ggccagtttg gcgagagtgg
    15421 tggttttccc caccccggtc gggccgatga aagaccacac tttgccaatc cctaggtccg
    15481 ccccggtcac ttccacctcg tccatgaact tccactggag gtagttgcgg taggtttctg
    15541 aattcctctc cgtccgggat tccttgagcc atatcttaag ttcttccccc agccgatgga
    15601 caaaatccgg ggctaagccg atcgagagca ttcgctccaa gaaggggtcc tcagagggag
    15661 aaaagcattc ttttttcttg gccgtcgggg gaagccacag aggggccacc ttcctcgccg
    15721 gcctggctcc atgcgccggt gcatggtcaa tcgcggccac cacttccacg gccattggag
    15781 aaaaagcccg ggtcctgcca ttgagtttct tcaaggattt ggtggagagg atgatggcct
    15841 cgggccccag ggcctccttc acccggcgaa gggcttgctg catgtccggg gcttcaaatc
    15901 gtttaatctg catgggcgac actcaccgtt cctagagact gaattttcgt ctgggaagtc
    15961 agctcgctgt gggacaggac cgccacctgg ggcaaaaatc gatccagcag ctttttcaga
    16021 tgacgccgaa cccctggaga gcataggatc accggctgat agtttagatg ggccacccgg
    16081 tctaaggttt ggtggatgct ggtcaggatt ttctgggcca cccccggctc cagagccagg
    16141 taagagcctt gttcaccccg gaccagcttt tcccgcagga ggtcttcgat tcttgggtcc
    16201 agggcaatga ccggcagggt tccctccggg gtctcaaact gcttgacaat cgaccgggaa
    16261 agcttctgcc ggacatattc ggtaaggacc tctgggtcct tcgtcaccgg gccatagtcg
    16321 gccagggtct ccacgatggt cagaaggtcc cggatagaca cccgttcccg caggaggttc
    16381 tgaaggaccc tctgaacgac ccccagggca aggttgttgg gtgtgagttc ttccaccgcc
    16441 ttggggtaat tctgggctaa ccggtccagc agcttctgca cgtcctggcg tcccagcagc
    16501 tcgtccgcat gtcccttgag gatttccgtt agatgggtgg ccatgatggt ggagatgtcc
    16561 accaccgtgt agccggagaa atgggcctct tccttccgct tctcggggac ccacaaggcc
    16621 ggcaggccga agaccggttc tttggtggga attccttcga tggttttctt cacctccccg
    16681 gggttcaggg ccatcaggta ccccatcatc atctccgccc gggctacctc attgcccttg
    16741 atgagaaccc ggtattcgcc cggatggagt tgcaggttat cccggatgcg caaggggggg
    16801 atgatgattc ccaggtcaga ggcaaactga cggcggaggg aacggatccg gctcagtaaa
    16861 tccccatgct gttcttcatc caccagggga atcaggccgt agcccacctc cagttccagg
    16921 cggtcgagct ggaggaggga ttcagccccc tctgccgagg gcggtggagc agcttttttg
    16981 gtttcttctt ccttcctctg agcctgtcgc cggttctcct ggagggtaag gtaggcaact
    17041 cccccaacca tcagggaaag ggtgataaag gggatatggg gcagcccggg gatgagcccc
    17101 atgaccaata ggattgcggc ggaaaggccg atggcctggg gctgaagccg gaattggcgg
    17161 ctgagctctt ttcctatgct gaattcggaa gcagcccggc tcacgatgat acccgccgcc
    17221 gtggagatga tgagggcggg gatttgggaa accagggcgt cgccgatggt cagaatcgtg
    17281 taggtctggg cggcggtcag gaggggaagg tcctgctgga aaaccccgat cacgaatccc
    17341 ccgccgatgt tgaccaggat gactaagatt ccggcaatgg cctcgccccg cacgaacttg
    17401 ctggccccgt ccatggctcc gtagaagtcg gcttcccggg cgatctgggc tcggcgcctg
    17461 cgggcttcct gttcgtcgat gaggccggca ttcaggtccg cgtcgatgga catctgcttg
    17521 ccaggcatgg catccaaagt gaagcgggcg gccacctccg cgatgcggcc gctgcctttg
    17581 gtgatgacca cgaaattaat gatcaccagg atgatgaaga tgataaagcc caccacatag
    17641 ttccctccca ctacaaactg gccgaaggac ttgatgacca ctccggcggc cgacgttccc
    17701 tcatttccgt agaggaggat gaggcgggtg gaggcgatgt ttagggacag ccggaaaaga
    17761 gtggtgagga ggaggaccga cgggaaggtg gagaactcca ggggatgggt gatgtacagc
    17821 gaggtcagaa gcaccagtat agagatggta aagctgcacg acaaaagaat atccaagagc
    17881 agcgtgggca ggggaatgat catgaccagc aggatggcga tcactgcccc ggccatgcag
    17941 acctcgctcc gctccaggcg gaagctttca cgggacaaga cgggggactg gatgactttt
    18001 tcggtcatac tcgtttatag ggttcggttc ttcatccggt acacatgggc caggatgtcg
    18061 gccactactt gatagagggt cgaaggaatg acccggccca gctccacggt tttgtacaga
    18121 atctgggcca gcgctttgtt ctccaccagg ggaatttgat gggcccgggc ggtttccttg
    18181 atcttctcgg caatgactcc cgcccccttg gccagaactt tgggagccgc catctcatct
    18241 ttttcatatc ccagggccac ggccagatgg gtggggttgg tgattaccac gtcggccttg
    18301 ggcacttcag ccatcattcg cctgcgggcc atctcccgct ggatggaacg gatgcgggac
    18361 ttcaccaggg ggtctccctc ggtctgtttg aactcctcct gaacctctct tttggtcatg
    18421 cgcaggctct tttcatagct ccatcgttga aagaggtaat cgaggacggc cagggccacc
    18481 ataaccagaa ccgtcttgaa gaagatgcgg gccgacacag agcctacgaa acggaagacc
    18541 ccgcctactt cctgatcgga gagagtcagg atttccggca attccttctg gaccgtcgag
    18601 taggccaccc atccgatgat cagaaatttg aagatggatt tgaccacctc caccaaggac
    18661 tccttggaaa aaaggcgacg aaacccctgg atcgggtcca cccgggagag cttgggttga
    18721 atgacttcca gagtgaacag gctcccgatc tgaacatagt tgctcaggac acccatggcc
    18781 aacacggcca ggagaatggg ggtcaggatt tggaagaggg cccagactac ctctccccgg
    18841 aaggctagga tattgtcagg gctcagggaa aaggtcccga tcttctggaa gctttggacc
    18901 ataacccaag atagacgctg caagagatac gtgctaagaa cgtagatgac cgataaggcc
    18961 accaggagga cagctacgga agggatttcc cggcttcggg ccacctgtcc tttttttctt
    19021 gcttcggccc ggcgctttcc ggttgccttt tcggttcgtt cgccaaacga ctcgtcggcc
    19081 attcctacct ctgcatcaat ggaagaaggg tgaaaaccaa agctcttccc gtttcttgaa
    19141 acagattcct aaagtaggga agaaagagct cgatggacaa ggccagaaag agaaacccga
    19201 ttccaagggt caggggaaaa ctggtgatga ggatattaat ttggggaacc gctttggcta
    19261 gaatccccag tccgatttgg gtaaagatta ggacggccat caccggagcc gcgattttga
    19321 tggccaccac aaaaagactc cccaggagtc cgagaaaatg gcgatagaga ccttcctgca
    19381 gatggatttc cccaggggcc aggaggagga aactctggaa aagagcccgg aagaaccagt
    19441 ggtggccatc caccgtcagg aagacgatca agctcaccaa gtcgagaaat tgggatagga
    19501 gtggggtgtc gacctccgac tgggggtcca ccacgctggc catgctcaac cccatttgga
    19561 agcccaccaa ttccccactg aactggatac cggcaaagac gatgttcacc gaaagagcta
    19621 aaaccagtcc gatcatcagt tcggcggcca tgaggaggcc gaactggatt ggctcggagg
    19681 gcaggagccg ggggtccacc ttaacgagag gcaggaggat gaggcccacg gtcagagtca
    19741 atcctgcctt taccaggctg gggatgctgc gtgaattcag caccggcatc atgaagagga
    19801 tgggtgagac cctcatgagg atcagaacca aagcctgaaa ttggaggagc gaccaattca
    19861 ccaaaaccgc cggcatctat cccttcccta cttgacgaaa ttgggcagat tcacgatcaa
    19921 ctgttccgtg taaaacatca tcttccgcat catccagggc ccaagaacca ggagagccac
    19981 aaaaacaacg acgatcttgg gaacaaagac caaagtggct tcctggatct gggtgaccgc
    20041 ctggaaaagg ctgaccaaca gccctacgat taacccgatt cccaggatgg gcaaagagag
    20101 atacagggtg gtttcgaggg cttcccgggc aatctgaccg acaaggtctg ggttcatttc
    20161 aagctctcca attcatgcgc gacgcctggg acctaggcga atgccgaccc caaaaactct
    20221 tccttccctt ggggtctgtg cccatggacg tctccttttc tattccacaa cccgatcttt
    20281 acttcttggg ccgtgcgccc ttaataaaag ctcttcacca gggacccgac cagcaggttc
    20341 cacccgtcca ccaatacaaa cagcagcact ttgaacggca aagagatcat gaccggggga
    20401 agcatcatca tccccatcga cagcagcacg ctggaaacca ccatgtccac gatcaaaaat
    20461 gggatgtata gaatacagcc aatctggaag gcggttttga gttcgctgat catgaaggcc
    20521 ggaagaagaa ccgttgtggg gatctcttcc tgattcttcg gccgggacat cttggccatt
    20581 tgaatgaaaa gggccaagtc cttttcccgg gtctgtttga acatgaattt gcgcaccggt
    20641 ttcaccgctt cttccagggc ctgagcctgg gagattttct tctccttgta aggggtgagg
    20701 gctttttcgt cgatggtctg ccacacaggg gtcatgatga agaaagtcaa aaacagagcc
    20761 agcccaatca gcagctggtt agggggcatc tggttggtcc ccagggcatg gcggaggaaa
    20821 gaaaagacca cgataagacg ggtaaaactg gtcatcatga ccaggatcgc cggggccagg
    20881 gaaagaaccg tcaggagaat cagaatctgg atgaggaccg aaacctctcc cgggcccttg
    20941 gcctctccta cgccgatttt cactgtgggg atgggccagg cttgggctga ggctgaggaa
    21001 taggtcatcc caatgacccc agccacgctc agcaggagga gccagggaag gagcttacct
    21061 ttcattcccc atctccgcca tttctcccct ctccttcctc ggaaagaggg ccagagattt
    21121 cagccagggg atcgcctccg gcttgaagcc aggcctggtc aagctcttct ggatcatctc
    21181 cttgttctcg accttggtca aaaaggtgat ctgctggggg gtaaccccca gggccaggac
    21241 ctctcctccg atttccacca gggaaacgta cttctgagga gcgatcggtt tggtggttaa
    21301 cacccggatt ccattgtccg gtcccaaaat cccctggact ctctgccagc gcttgaaaag
    21361 gcggaccagg agaaccaaga gggcgatcac cacccccagg gccaggacca ttttcagggt
    21421 tgcccaaagc atcacttcct cacgatccta gttgtttcac cctttccatc gggctgagga
    21481 tatcggtcat cttgattcca aacttgtcgt tcaccaccac cacctctccc cgggcgatca
    21541 gtttctggtt cacgaagatc tccactggct ccccggccag cttgttcagt tcgatcaccg
    21601 atccctgtcc cagttggagc agttcgctga ccagcatcct cgcccgcccc aattccacag
    21661 agacctccag ggggaggtcg aggatgaaat ccagattggg gacttctttg gggcttcctt
    21721 cagccttcgg ctcggccccc ggtttgatcc ccggttcttt tgtgtttccc aggttttcaa
    21781 gctccatggc gcggctcctc attcacgcag gtttgagggt tccttcgatc tggaaggctt
    21841 tgctgctccc gtaaacgccg gcccttccca gaaatttggg aaccccctgg attttagccg
    21901 tcaggggttc ggagacttcc tttcctaggg gcaggatgtc ccccgctttc aaccctagaa
    21961 gcttctcgga agtgaccacc gccttggcaa attccaccac cacttcgact tcggtgaact
    22021 tcagctcctc cgacaacctg gcaatccagt tttgatccaa ttccatatgt tcgctctggt
    22081 atccggaata gagcttgcct ttaattggtt ccaggctgaa atagggaatg cacagggtga
    22141 tgaacccggt aatctgttcc acctccaggt tgaaggagat ggtcaggacc agctcgcccg
    22201 gaggaacaat ggaggcaaat tggggattga cctcactgcg gacatgctga aacgagaggg
    22261 gatggaccga gttccaggct ttttccagtt ccacgaagat gatgttgacc actttctgga
    22321 taagctttga ttcgatgtgg gtaaaatccc tcccctcgat gcgaaaattc gtccgacccg
    22381 tcccacccag aaaaatatcc acgaagctga agaccagctt ggggtccagg atgagcaggg
    22441 cgtggccccg caacgggttc atcttgaaga cctgaatgct gcagggcaga ggaagagtct
    22501 ttgtgagttc gccatatttg gtcagaaggc ttttcccggg gacgatatcc acatttttcc
    22561 gcagggtgac cgataggggt gtccgcagga gccgggcaaa ggaatcattg atcacctcca
    22621 gggtgggcat tcggccgccg ataatccggt cctgattggt aaaatcataa gccgcgacct
    22681 tggattcctc cttcggctcg ggggtcacct ccacctttcc gccttcaatt cctttgagca
    22741 gggcgtcgat ctcgtcctgg tttaggattt tatccatgat cgttctactg gattacgaac
    22801 tccgtaaaga aaatattctg cgccttaagg ctgaccagca actggttcac ctttcccttg
    22861 atctcttccc gcagggctgc cttgccttcc gaagtcatta tttccctata ttgtctgctg
    22921 gaaagaatgg tgaggatcgc atcccggagc tggggctgcc ttttttcata ctcctcgctg
    22981 ggtttttctt gattgctctc aatatgtaaa gtgaccttga gatatcgggg ttgcccgggg
    23041 tctgccaggt tcaccaggaa gggctccatc ttatagatat gcccgtttgc ggcagcggtt
    23101 gcggggggag gagaaggttt tcctttgaaa aacaagaacc atgctcctcc tcccactgcc
    23161 cccaggagaa cgatccctat ccctatgatt ataaataatt ttaaagactt aggttttttc
    23221 tctgacttgg gattatcggc catttgccac cattcaggaa tggtttttat gattccaggc
    23281 ccgccagctc agaacctatg tccatttttg caatgggcat gccaagctgg agtaatccgg
    23341 gttatagaaa caacatctca atttaattta aattttcagt aaaagggagg atatctgaaa
    23401 gtttgaattt atgtcaattt cttctagctg gtcaatttgt tgacgtacct gaaaagtgcg
    23461 aggtcagcgc gggagggatt ggaaaattat caattttttt aatgaattat ttgacttagg
    23521 gggaggggag cataattact gcctatgctc ccccattcct gcaggagaag aaccctactt
    23581 gatattgatg agctgagcca gcatctcatc ggtggtggtg atgatctttg aatcggcctg
    23641 gtaggccctc tgggttatga tcattttgat gaactccccc gccaggtcca cattcgattc
    23701 ctccagcata gaggcggaaa cgttccccac gcccccttcc cccgccttat tgatcaaggg
    23761 gtccccggag tcgggcatgg acctgaagag aagagacccc gctctttcca acccatacag
    23821 actgggaaaa tctgccaacg ccaggcgagc gatgtttttc acttgcccat tggtatagtt
    23881 ccccacaatg ttcccctcat tagtgatact gattccggaa attgttccgg ccccgtaacc
    23941 attctgaagg agactctgaa tcgcggaagg agccccgtac tgggtggtgc cggtaaaatc
    24001 agccgaaacg gccgaagcag cgaccccatc ggcgtaggta atggctcccg tgtagagggc
    24061 cacgggcatg gccgtggtaa gggcaccgga gctgtcgaac gcgatcgttc ccgtgccaaa
    24121 gagttggggg gtgcccgccg ttcctccgct cacatcggcc ccatcgatga gggcattcca
    24181 actccaggtc tgagcggcag tctgggtaaa atacacggtg acggtatggg tctggccgag
    24241 actgtcatag accggcaagg cggtggaata gtgagaagtc cctcccgggt cggcgatatt
    24301 gaaagtggtt ggggtctccg ccgaattcag gtttaagacc atttgcacgt tgctggtggc
    24361 cgcaggggcg atcatattcg tctggtccac gacgatgtct gacaggcttt cgatcaggtt
    24421 tgttcccgta ggatcataaa ggtacccctg cactcgaagg ccgttggcat ttacaaagta
    24481 gccatccttg tccagatgga agctgcccgc ccgggtatag aaatcttcat ctgtagttgt
    24541 attcttcacg atgaagaagc ctttcccgtt gatggccagg tcggttccta cgcctgtggt
    24601 ttcaagggtt ccttgggtga agctgcaggc catactggaa actttggctc ccacgcccac
    24661 ccgagtaacc ccgctgatcc cctccaggga catccccaaa aggtcttcga attggacgct
    24721 agaccccttg aaacctaccg tattgacatt ggagatgttg tccgaaatga cctgcatggc
    24781 cacgctgttg gagtccatcc cgctcagggc ggagtaaaag gaagaggtaa tcgacataaa
    24841 atactccttt cttcaaaagt ttagttcatg attgacaaga tgtcgctaaa ttggactttg
    24901 ccgccgttca cttggagagc gaccgagcca ttatccagag aaaccccatc caccgtgccg
    24961 acctggtagg tggtaaccga aaccgctttc ccctgggtat caaccgccag aaccttgaag
    25021 gtgtaggttc catcggggag ggttacccct gcctggttct ttccgtccca cccgatcttc
    25081 tgaagggaag cgtctttgaa tcccgcatct atctgccgga ccagagtccc ctgagaatta
    25141 aagacctgga cgaatacctt tcccgggttg gccaactgat aaaacccttc tccaaccgtt
    25201 ccttgctgaa tggccagacc atttccctga gtcttcactt tctttccgat taggctggca
    25261 acctgtaaat tgttcatcga agtctgttcc gtcgccagct gggtcaaggt ctggttcatc
    25321 ttggtcaagg catctacggt attgaactgg gccatctggg tggccatctg gaagttatcc
    25381 agaggctcca gggggttttg gaactgcaac tgggtggtga agagcttcaa aaagtcttcc
    25441 tgagttaggg tgttttttga ggccttttcc actccctggg aatttgagct cgaacttgtg
    25501 ccaactgcag tcacgctcat ttttcatcct ccttccatag ttaaaccaat aaatccagat
    25561 atcggctcgc cagatcgggt gccggagttc gacctggaat ggactccatg gagtttgctg
    25621 atgaagcctt ttcttcaaca ggcatccggg ctttccagga atccgggttt ccggtgtctt
    25681 ctttccgccc ctgaaaccat cccgggtcct gctgaacgaa gacatcaaac ttttccagtt
    25741 taaagccttc actctcgatg atcctctgaa tctcgccctg gctggtttcc aggttggctt
    25801 tagtcgtggg attgtccgtc cagagggtgg tctttatgtg ctctttgtgc cggtcaatct
    25861 ccaggaagac ctgcccgagt tctgggggct ccaaggtgat ccgaattctc tcttcattgt
    25921 tccggaccaa ccacaggacc ctctggccca cttgctgaaa aaccgcttga ggatcgggtc
    25981 tctccgaagg gggtggggag gcttccttga tctcccctgc atattcaaca tggcggttca
    26041 ggggatcggt caacgccgcc aagaaggacg gggggttctg aacctctctt gcggcaaggg
    26101 gacggatatc gggtccgacc caaagaaact tttcctgccc ttttccggca tctgcggaga
    26161 aaggagtccc cgactcttta tctggtgagt ccaggatgta agcctggttc ccgaccaggg
    26221 gacctctttc ctgcaggttc tgtggaatct ctggccccgc tgcctccttc gctgccccaa
    26281 agaggggtcc ctgttttttt agaacctgtc cttcaccggt cctggggttt gcctccattt
    26341 tcaagaaagg ctcgaaactg gggaagaaat ctttcgattc ttggacaggt cttctttcac
    26401 tcccgttgct ttcttcctgg atcaaaggac ggatgaaatt cttttcccct cccgcggata
    26461 actccatttt ctcggccgat tctttccgaa gagactcttc ccaagagact ggggcgattg
    26521 gagaatcaga gaaggggacg atcttctttc cgctatccgc gggcatatcc attttcccag
    26581 tcgattccct tagaaggacc tcttccaaag agactggggt gtttggagaa tcggagaagg
    26641 ggacgatctt ctttccgctc tccgtgggca tatccttttt cccggtcgat tctttcaaaa
    26701 ggacttcttc caaagagatt gcgggggttg atggatcgac tggatgggct gtcttccttc
    26761 ccttctccag tgccatttcc aggttcccgg ccaatggtct ctgaaggggc tcttccagtg
    26821 aggtctggga gattgacgaa tcgagaggaa tgggtgattc ctgcaaggtc ggacccgctt
    26881 cttgaacccc ggaagagatg gaggatataa aagaatagat ggccggaact ggattctctg
    26941 ggctcttgtc gccactgggg ctcatttcct gatttgtttc tccatttgcc ggatgagacg
    27001 aaacgggtgg ggcaaaggct cctttatctt gatcaggggg aacggacagg gaaggcattt
    27061 cattcggaag gggaactggg ttccccccat tcatactagc cgctattgcc tccaactcag
    27121 acccgaggga agatttggag aagatgaaaa ggggagagat ggccatctgg gaaggagccg
    27181 taaatccttc tttcttggaa agagaggaaa caggtgggct aaaggctcct ttctcttgat
    27241 taggggaagc ggacggggaa agcttgtcac caggaagggg tactgggttc cccattttca
    27301 tactagccgc tattgccccc ggctcagacc cgagggaagg ttgggagaaa atgagcaggg
    27361 gagagatcgc cgtctgggaa ggagccgtaa atccttcttt ctcagaaaaa gcggcaaaac
    27421 cttgatcaga gatcggtttc tctgggggga aagcgacccc attggggacc aggattgcag
    27481 gcaggatatc attggccgtc gtcgaagacg ctgggtctcc aagggagctt ttgtggtcct
    27541 ccgaaggtgg attcccttcc ttgaaatatt gttttgccag gactttggcg aaattcccaa
    27601 agccctgggg ggcatcttga gcgggaaagc cctcggggag atcagaggaa gaagcaatgg
    27661 gtaaaagcag ggtttccatg gtttggactt ccttctaagc aaccccagtg ccatagaatc
    27721 aaaaacccag gtgtaaaaaa ttttcttttt atttcaatag gtttaattga gtagccaaga
    27781 gaagttttct tctctctctc cgtccccggc aagaatttcc ttttcaattt ataaaaagga
    27841 agaaaaattt tccggatgga aaggaaaagc cgaatttcaa ggtttctacc cggttgagtg
    27901 agcaggtgcc aaacgttcct acccgaataa actccgagag ggaaggtcaa ctaccgaatt
    27961 agccctttga attattggaa accggggacc tgcttggaat ccgttctttg ctctggggag
    28021 ccgaaggcga atgaagactg agattaggag atatcccctc tgggccgggg ctgatatttc
    28081 aaggttgtgg tttcatcggc tcgcttctgc tcggcttttt gcaggcggga gaggaattct
    28141 ttgagctgtt tctctttcaa cctttccagg gttttctttt ctttggttaa agccagcagt
    28201 tttcccctct cctgatccat ctcccgaagg atcctcctct tctcatcctc cttggagatt
    28261 aattcttttc ggctctcttc accaaactgt ttaaagagaa cgtactctcc cgccggcatt
    28321 ccttgttcgc tcttctcctt caatttctgc tcgcaggcca ggcgttgcag ggtgagtttt
    28381 tcgatctctt cttccttagc cttcaggcga gtcatcattc cggccagcct catctgggac
    28441 tgttcttcca ggcttttttt ccaattcagg agaggttgga gggaaaagtg gaagggcatc
    28501 gtttatccaa gaactaaatg gattccccgc cccatcggag cgggttgaaa tgcgggtcca
    28561 agtcgccccg gactcccgaa tcaaccgacg atggctttga gacgggccag ggattctgct
    28621 agaccaacct tttccgccac cccctggcgc agaaaagcgt tgatttcagg catcttcttg
    28681 atcgcgtaat caatctgggg attcgtgcca gtcacgtagg ccccaatgtt gattaagtcc
    28741 tcggaccttc ggtaggtagc catgaccgaa accagcttgg tccgagcgtc cgtctgctca
    28801 gggggcacga tgtcattcat cactcgacta atgctctcca gcacatcgat cgccgggtaa
    28861 tggccctggt tagccaagtt ccgggagagg acgatgtgcc cgtcggcaat ggatcgtatg
    28921 gcatcggcca ccgggtccat caggtcgtcc ccttccacca gcacagtata aattccggtg
    28981 atgctccccc ggtcgggggt cgttcccgcc cgttccagca gtttcggcag ttgggataaa
    29041 acgctcggtg tgtacccttt ggccgtgggc ggctcaccga tagccagacc cacctcccgg
    29101 gaggacatgg caaaccgggt gatggaatcc atcatgagaa gaacatcccg gccttggtcc
    29161 cggaagtact cggccagggc agtggctaag taggcccccc tcatgcgcag caacgggtgc
    29221 tgatcggagg tcgccaccac caccacggaa cgcttaaggc cttcctctcc aaggtccttc
    29281 tcgatgaatt cccgcacttc cctcccccgc tccccgatca gggcgatcac gcttacttcc
    29341 gcggtcgtgt gccgggccat catccccaga agggtgctct tgccaactcc ggagccggag
    29401 aagatcgcca tccgctgtcc ctttccgatg gtgagaaggg cattgatggc tttcactcct
    29461 acatcgatgg gctcgtggat tctcttcctc aggacggcgc tggagggcgg cgcgtaaaga
    29521 ggggcctcca cttctttgga gatgggtccc ccatggtccg tcgggttgcc catggcgtcc
    29581 agtacccgcc ccaggagggc ttggcccact ggcgcctgag ccgggaaacc cagggaaacg
    29641 attctgcagc ccggttcaac ccctcgaacc tcccccaagg gcatgagcag gattttctga
    29701 ttccgaaatc caaccacctc ggccggaatt gctccctctt tgccttccga tagaatttga
    29761 cacatgtctc caatagccgc catcggtccg gttgcttcaa tcaccaggcc gatcaactgg
    29821 ttgacccgcc catagcgccg gatggtctcc cccttttgga tggcctgatg aagggaagcc
    29881 cagagagaat cgggaacagg ggtcatggcc gggaaaggtc cgaaggggtg aggggtgggt
    29941 caaatgaaga acccacctgc gagagaatct gctccagctg ggtctccaga gtggcatcaa
    30001 tctctccaaa ggaagtctcc aggaagcacc cgccccgggt gactgtagga tcggagatga
    30061 atcggatgtt gtggctctct cctcccttcg ggtcccaggg caagccctcc gggtgggtga
    30121 tcaagtgctg gtggtcttta gggtgcaggt ggatgattac gtttcggggg tccaccacgt
    30181 gttggaaagc ggcctgaagg gtctttagaa gggtctcctc ccggaggggc aaatccaggc
    30241 gcaagattct cttggcaatg gcgaagacca ggagcaccat ttcccgctca taacgtcggg
    30301 gaagatcctg ttggatacgc cccaattcat ctgcgagctg gcggaaggaa tcgaagaccg
    30361 tttcccatct cttcctcccc agctcggccc cgtccttttc cccctggaca aatccctttt
    30421 cgtaggcatc cttttcaatc tccagggctc tctcccgggc ctgccttagg attttctctt
    30481 ctttttcctt catgccctgg gaccattgca ggttctcccc ggaagtctgc aggcagggcc
    30541 attctacagg cacaacccga ggttgtccgg cttcaggccc caggccctcg gccatgaatt
    30601 cccgggaggg ttctacaggg gaagagaatt gagcgatgaa gaccttagac aaagacatcc
    30661 tccttgccct tcccggtcag aatcactttt ccttccgctt ccagcttctt cccgatcttt
    30721 atgatggcct gctgggccac ttccacatct ttgagacgca ccgggcccat gacttccaag
    30781 tcttccttca tcatcgtgac cgcccgttcg gacatgttct tgaaaatcct ttccttgagt
    30841 tcatcggaag ccgttttcag agccaccttg agggaatcgg cttccacttc tttcagaatg
    30901 gccatgatac tgcggtcatc cacattgatg aagtcttcga agacgaacat ccaccgacgg
    30961 atctcgtcag ccaatccctg ctgctgctcc tccatggcct gaaggacctg agtctcggcg
    31021 gaggggtcca tttggttcag aatctccgcc gccgaacgaa cccctcccac ctgccccccc
    31081 tccacgtttt cgaacatgga gatttcctgc tgcaggatct ggtcgatttc cgccagaatg
    31141 gctgggggaa ctttttccag ctgggcgatt cggtagagga cctcattttg gaccctttcg
    31201 ggtaactctt ctagaatcgc ggcggcatgg ccggggtcca ggtgggcgag gacaacggca
    31261 atcgtctggg ggtgttcatt gtggataaaa ctggccaggg ttctcgcgtc caatttgcgg
    31321 accttctgga aaagattcaa tctttccttc tgctgaatct cttcgatcag tgcctgagct
    31381 ttctcccccc ggatggcctt ggagaagaca tttttgatga aggcgcttct ctgccccggg
    31441 ctcagcgcca ggctctgagg agaggccgcc agttgatgga attcgtcaaa caccgagttg
    31501 aagacctccg gcgccaggga gaagacccgg gacatggacc cccccagctt ccgcagctca
    31561 ttttcatcga ggtttttgac gatctcggcc gctaaatctt ctcccaagct gaagagaaga
    31621 accgccgcct tctgagggcc ggaaattttc tcctgagccg gggcttgatt aggcgtcttt
    31681 tccttcattg atccattcct tgatgattcc aaccgccttg gaggggtttt cctggatcag
    31741 ctgcaccgtc tgggttttaa ggtctaacgt tttgggctgc ggggagaggt ccgggggtag
    31801 ggcgggcagg ggagaagtct tgggaagata ggccgcctcc tcctggggca gggaagcctt
    31861 cctccggagg agcggccgga ccacgaagaa aatgaagagg accgcaagga ttaggctgac
    31921 caggggtttg taagaaataa ggatgtattc tttccaccat tctccggcca ccgccttgct
    31981 ctcctcttcg gccacagacc agctgaaaga catgttgatc acttccacct gatcgccccg
    32041 tccttcgtca tagccgatgg cttttttcac catggcttcg atgctcttca tctcctccgg
    32101 ggagcgggcc tgatactccc ggcccttctt tcctttggca tcggccactt ccttgtaggt
    32161 tccgtcgacg atgaccgctg ccgagatctt tttcaagacc cccacgggag acttgatccg
    32221 cttgttgact cgggagattt cgtaattccg gacctcgttc tgccgctccg aatgattgga
    32281 ttggacggga acgggttcgg cggtccgcgc ctttgtctcc ggagccgctt tccctttctc
    32341 ctccggggcc gggttccctt tctcctccgg ggtcttgacc gccggcaggt tctcgggctt
    32401 tccgccggcc ggggcccggg cgttggatat catggaggat ttctctgaat tcttttgctc
    32461 actcctcacc accgaattgg ggtcgaaccg ctcttcagtg atatccacct gctgaaaatc
    32521 gatgtccgct gacacccggg caatggcctt gttgaagccc agcacttcct ccaacatgcc
    32581 ctgggctttc ttcttcaggc cttcctcgat atttctctga taatccagct gatgactggt
    32641 catctgcccg agaagggtcg tttcgttccg tttgaatagg atctttcccg aggtgtccac
    32701 catggtgatg ttgcccgaat ccaacccttc aaccgcgctg gctaccaggt gaacgattcc
    32761 ctccacctgc ccggcgtcca gcttcattcc cgagcgggtc ttgatcagca ccgacgcgct
    32821 gggctttttt tggtcttcca ggaagagaga ttccttgggg gtgacgatat ggacccgggc
    32881 ttgctctact tccttgatct ggcggatcgt gcggctgagc tctccctgga gggcccgttg
    32941 gtagtttagt ttctgcacga agtccgtggc tcccaggttg gaccggtcga aaacttcgaa
    33001 gcccacccct ccccccttgg gcagcccttc ggcggccagg gtgaggcggg tgtcgtagac
    33061 ctgttccctg gcgaccagga tggaagtccc attggaggag agctggaagg gaattttctt
    33121 ctccttcagt ttgttcacca tctccccggc gtcttccgtg gagaggttgc tgaagagcac
    33181 ctggtattca ggcttgctgt tccagtagat cagggaaccg aatccgatga gggaaaaaac
    33241 caacaccaga gcgatggaca ggttccgctt ccaagcggga gagccgatcc attgggagat
    33301 tgggttgaac aggggattgg ccatggacct ctatagggta agattagaat tgcatgcgca
    33361 tgatctcttc atacgcggtg atcatcttgt tccgcacctg gaccatcagg cggaaagaaa
    33421 ggttggcctg ctccagggca gtcatggcct gatggatgtc aatctttccg gcgaccatct
    33481 cctggacgct ctggtccgct tcctggagaa ggccattgac ttccccgatg gatcgaccca
    33541 gaatcttctt gaagtccgag atggggtcga gaacctcgga ccgatccgcc ccggcctttt
    33601 ccaggggaac cgaggaaatc tttcgaatgc gcagatcatc catttccatc ctcgctcagg
    33661 actttacttg cttccgatct ccagagcccg cagggccatg gtcttggccg cattgaacgc
    33721 ggccacgttg gcctcgtaca cccgggaggc gcccatgaga gccaccatct cttccatgag
    33781 gttcacgttg ggcaggagaa ccagcccggt ttcgtccgca tcggggtggg aaggatcgaa
    33841 gacgctcttc aacccatagg gatcttcaac gatatccgtg acttggactc ccagggcctc
    33901 cggcctcatc cgggagaaca ggagttcggg aaaagaagag ggtacggggg tgggcgacag
    33961 gatggcccgc ttgcgcaggt acggtcctcc ctcttcggtc cgggtggtct ggacgttggc
    34021 catgttctcg gcgatcaccc ggagcacggt ccgctgggag ctcagtccgc tggcgctgat
    34081 ctgcatggat aggaaaaggt tcattcttta cctcgattct tcgatcccga ctttcaaggc
    34141 ttcgaacttc ttaatcaaag cctggacccc ggcctggaac atgagattat tctccgctag
    34201 tcgggacatc tgctgatcta tgtccacccc tcccggcccg gggtccatct cgatgtggga
    34261 agagaagtcc tgggaaagag gtccttcggt cgccggcaga tgggccgggt gggtcttgcc
    34321 cagaggaagg atggaagaat tttccaggca gccctcgaag accttttgaa agggaaggtc
    34381 ccgggaaacg tactccggag tttcgatatt ggccacattg ctcgagagaa cccggtggcg
    34441 ggatgcccga atgtccatgg acctctggat cagggacatc gtcttgtcga acaaattttt
    34501 tccgattaag aggtggttca tagccgattc agtttagcaa ttcttgtgcc aaggggtttt
    34561 caaccgggga aaattgaacc tttcaggaaa gccctgctgg aagttctttt ttgtattcgt
    34621 tcagtttgtt cctcagggtt cggatgctga tgcccaacat tttagcggca tgagtccggt
    34681 ttccctgggt tttctccagg gctttggaga tcatccgctg ttccatatgc cttaagcaaa
    34741 aagaaaccga ttccgccccc tccgctcccc tgttcttttc cacctctccc ccggtgtctt
    34801 cccgaaaatc ttgtatctcc agtgtcttcc gggatgagat caggaccgcc cgttcgatga
    34861 cgttcttcag ctccctcacg tttccccgcc actcctgctc cttcatccag gtcagggcgg
    34921 cctcggaaat agaacggccc ggccgggcat attcgtcggc gaattttctc aaataatgat
    34981 aggttagcac ttctatatcc cccgggcgct cccgcaaggg gggtaggatg acctcgatca
    35041 ccttgagccg gtaatatagg tcctctcgca attctccttt tttcacccag gattccaaat
    35101 cccggttggt ggtggcaatg acccgaaggt cgatgggaat gggacggttt cccccaatcc
    35161 ggtccacctc gttttcttga aggaccctta ggattttggc ctgcagaaag gtattcatct
    35221 cgctgatttc atccaggaga atcgtacccc ggtgggacag ttcgaatttt ccaatcttgc
    35281 gggccatggc tccggtgaaa gccccttttt catgtccgaa aagttcactt tccagaaggg
    35341 tttcgggcaa ggaggcgcag tttacagcca cgaaagggcc ctgattccgc tggctctgtt
    35401 catggatgta gcgggccagg agctcctttc ccgtcccgct ttccccctgg atgagtaccg
    35461 tggccttgct cggggcgatt ttcccgcaaa gggcgaggac ctctttcatt tttcggtctt
    35521 gggtgatgat gggaactcgg gatggagggc agggggattc tgcttcaccc atttgccccg
    35581 agaagctctg acgcagagca ttctcgatgc cttgctccag ggtctctaag gagaagggct
    35641 tcaggagata atcaaaagcg cccttcctca tggcttccac cgcgttctgt acgcttccga
    35701 aaccggtcat caggatgacc ggcgtggggt gggaatagcc cctgacatgg tccaggagct
    35761 gaaggccgtt catgtggggt aggcggatat ccgaaacaat caggtcaaat gctttctggc
    35821 cgattaggtt cagggctttt tcggcactct ccgccagatg ggccgaatat ccattctgcg
    35881 aaaggctttc caggagaact ctccccaatt ccggctcgtc ttcgacgatc aatatgggtc
    35941 tgcgattcat gaagaatctt ttcgaaggat tttcgccaaa agctacgggg gcttccccct
    36001 ccgtgcatgg aggacctttc agcaagacct atgccattga ggatcttaaa aaaatgaagt
    36061 cattataatc ggttaaaatg aattggaaga atcaggaagg tcaaaaaaac aatgatcgtc
    36121 acatttttga cagtagctaa gattacttga agaatagatg aaatgaaaaa agggaagcag
    36181 aggagactgc agaggggtcg attggcgaga ggaaaggata ttagagtttt ctcctgcctc
    36241 gcccagagtg caagccctga ccttcaaatg ggccaggcac cctccgcatc caatattaat
    36301 tgtccgggcg ctgcaggttc tgtcgtttga tcttctcgat gagcgtagtc cggttcagat
    36361 tcaacagacg ggcggccttg ttcttcaccc aacccgtctt catcagggcc tggaggatca
    36421 aattcttctc aaattcgtta accgcagtat ccagggagat tccttcctcg gggatctcca
    36481 gcaactgggg gggctcgggt tggggcaagg aatggaactt ttccggcagg tctggggcgg
    36541 cgatcacgtc atggtccacg agaatgacca cccgctcgat ggtgttttcc agctcacgaa
    36601 cgtttcccgg ccattcatac ccgatcaagc cttccatggc tccgtcttcg atggcccgga
    36661 tcttcttctt cttgctccgg ttgaaatgat ccaggaaata gtgggccagc agcgggatgt
    36721 cggtccttcg ttcccggagc gggggaaccc ggagggggat tacattcagc cggtaataga
    36781 ggtcttcccg gaattttttc tgctgcaccg cctgtttcag gtcttggtgg gtggcggcga
    36841 tgatgcggat gtcggtcttg atcgttttga tgccgccgac ccgttcgaac tgcttttctt
    36901 ggaggaccct cagcaattta ctctgtagca gggggttcat atcgccgatc tcatccagga
    36961 aaaccgttcc cccattggcc agttcgaacc gcccgatgcg ggtgcggatg gcgttggtga
    37021 aggccccttt ttcgtggccg aagagttcac tttccagaag ctcgctgggg atggcggcac
    37081 aattgaccgg cacaaagggc cgctctctcc ggtagctgtt gtagtggatg gctttggcga
    37141 tgagttcctt tcccgtcccg ctctccccca gaatcaggat ggtactatcc gtatcggcca
    37201 ccttctccac gatctcgaag accttctgca tcttctcgtg atcgccgatg atgttttcga
    37261 atttatattt tttcttcagc tggttcttga gattgatgtt ttccaccttc aggctccggt
    37321 attccagtgc cctccgcagg gtgaccagca tttcatccat ctttactggt ttggtaaggt
    37381 agtcgaaggc ccccatcttg atggcttcca cggcattttt gaccgtcccg tacccggtca
    37441 ggatgatgca gatggaatca ggagagtgct ccatggcaaa ctccagaact ttcatcccgt
    37501 ccattccggg catcttgagg tcggagataa tcaggtcata ggacttcttc tcgaccatct
    37561 cgatcccgct ggccccatcg gccgaggtgt ccacttcgta gccttcggct cttaggtatt
    37621 cggaaaaaag agtaagaatt tccggtgagt cgtcgatgac cagaattttt tcgttactca
    37681 tttcctctcc ctttgcctcc atttgaaagc catcttacga tatgtacaat tttatgtcaa
    37741 ttcattattt taggaggaat tgaagttaat ttgaatactt aactgaagag cctgtcggat
    37801 tttttggcag ggacaatttc ggcgtctgat tgggggttga acgattgaca gttgtgaaaa
    37861 ggctgggcaa cagaactgat gaacggccta gcgccggtga ttatcgatga tgatattttt
    37921 caggatatcc caggacctct cttctttctc tttttcttct tgcttggctt ttctctcctc
    37981 ttccggagtg atgatggagt aatatatctt gtcttttcct ccatcctcgc gcgggacctc
    38041 tttcgctccg attccttcct ttggctccgg ggaaatcctc tccggttctg ttcctggcgg
    38101 gactttgccg ggttgggcac aggccgtcct ttcccccccg gagaagaaac taaggaggaa
    38161 ccctaggagg acaatgagaa aagccggcct ccgatttatt gctgaatgag acctcaaccc
    38221 agaggctctt gtggatttct cagccaaaga tggcaccctg tggtgacgcc cgatggagag
    38281 tagaccctta gacgtagtct ccccggttga acttttcaat ctccacgttg gaaaggattc
    38341 cgatctcccc catcattttc cgcaggggat cggagggggg caattgatcg ctccatcgat
    38401 ccaactcctg aacttcctgc cgcagcgact ggaccagagg atacacctct ttcagagaca
    38461 cccgaggatc ggacagggcc gcttggtacc gctcgagcag gttcagggtc ctctccgtca
    38521 cctccgtccc ctgggactga ggcgaagggg ctcccactgc caaatttgag gggagcaatt
    38581 ggaaggttac cggttcaagc cattcggccc ctcccgcacc tcctgtggaa gtagagggcg
    38641 atggagactc ttttagatgg gaatgggctt cttgcagagc cttttgaaaa tccgctcctt
    38701 ccatctcttc cctcttcccc tttttgccaa aagccaaagg gggaccgccg atctctttca
    38761 catccatggg cactttcctc ctgatgattc taccttgggt agaatctttg caagttcaat
    38821 gccgggcatt tctctgcatc ctcttaatct gcccggtcgg atggcagatg gaaagtaacg
    38881 gaatatttcg agaaataaaa attgcggggg gaaagaccta tctgattcac acccttgaga
    38941 ggcaagaaac gttgaaccca tggcgccgct gggcaatttt tgcccggtgg gagggatctt
    39001 tctatcgggg ctccctctcc ctgcccctac cggtctatca gccttttcat ttcggcgcgg
    39061 cccggctatc ttgccttact ggagtaaaaa ttcatcgaaa tagatttgcc tgatcctcgg
    39121 cgaggccaga gtcttttccg cctttaccaa tagctccttt ctcagttcct ctctcccctg
    39181 gggacctttc agatcggtca gtttcttatc gccaagggtc acgattatca tgtccgtgag
    39241 caagggtatt cttttctgaa cttcgtccac ccactctttc tgagccacct ctaggacgat
    39301 cgtggtcctg atgtaatggc ggcagctggc ttcgttcaaa ttcacgacca gcgagcttag
    39361 cttgatcatg ggccctacat ccatggccga ggggggatgt tcttcccttt tctcccccgc
    39421 ccatgcccgg ccaatccctg ggtcggaaaa aagggccatc tccatccagg gggccaggaa
    39481 aagaaacgcg gccgtcatcc ccaaaaagag aagccccccg ctcttcagac tttctctttt
    39541 tattcctctc ttggacaatc ttcattccct cgttgggcag gcgagagcga ttcaagttct
    39601 gagccaagga atcccgggca ttcattaggc tgctaccgtc gttccttccc cctgggccgg
    39661 ttcaggttga ctgagcccta cggacgattt cctgggggat cttttccagg gacccgatcc
    39721 actcaaccgc tcccagcctg atggcttcct tcggcatccc gaacaccacg cagctggcct
    39781 catcctgggc aatcgttcga gctccggcct ctttcatccg gagcattccc tcggctccgt
    39841 cggaccccat tccggtcagg atgaccccga cggcgttagc ccctgcatgt tgggccaccg
    39901 agttgaaaag gacgtccact gctggcctct ggtgatggac cgtgggacct tccgtgatgt
    39961 tcacgaaata cctcgcccca cttctcttca ggaccatatg gaagttccct ggggcgatca
    40021 aggcctggcc gttcatcacc ccatctccat ccttcgcctc ccggacccgg atgccgcaga
    40081 ggctgtccag ccgctcggcg aaggattggg tgaagtaggc cggcatgtgc tggacgatta
    40141 cgattccagg gatgttcggg ggcatctgca tcaggatttc tttgatcgcc tcggtccctc
    40201 cggtggaggc tccgatggcg atcacttttt gggtcgtctg gctgagcgcc ccgttctccg
    40261 ccccgcgcat gagcggagga ggagccggtt tggatcgcgt ccggtggata ttggccctgg
    40321 ccgccaagcg gatcttctcg atcagctgaa ggctcatatc tcccaccgaa taggccgaat
    40381 cggctttggc caggacttca atcgctccgc tctccagggc ctccaccgcc attttgcttc
    40441 cctgaggagt cagcgaactg accacaataa ccggaagggg gtaatactgc atgagcttct
    40501 tcagaaaggt cagcccatcc atccggggca tttcgatatc cagggtgatc acatcgggct
    40561 tcagctgaac gatcttgtcc cgggccacaa acgggtccgg tgcgctgccg accacttcta
    40621 tatccggctc tttggacagg gcttcggtga agacctttcg tacaatggcc gaatcatcca
    40681 cgatgagaac cttgattcgt tgtctttcca tgtctttgtt cccgatcccc gactctgctg
    40741 gttcttccgg ctttctctgc gaaggaccgt cagggttcca gttggatcat caatttgacc
    40801 cgttccccat cggcggaaaa atgaattacc atccccccgt cctgaggctc tgtctgggtc
    40861 tgggtctccg agaccctctc ggccttggtc ttggtgagcc ggtactcccc cttccttttc
    40921 aggaggaaaa agagatttcc ggagatcatg ttgttgagct ctcctatcat gtccagaatc
    40981 tccgggtcgc ccacttctgc ttcattcatt cccatgaggt tcccggtcat cttccgcgcc
    41041 aatccatcag ggaggattag gatgatcttt cccgagggaa ctccgtcgaa ctcgatctcc
    41101 gcccggatgg aagcggcaga ggggttctcc tccccccctc cctgagcttc ccctcctctc
    41161 gtatcgcaag actccccgac ctcttcaggg aagatgaaga acatctcctc gaaaaccttg
    41221 gaaacggccc ccacggcgct ctgacgaatc tcctcaaaca cttttctcct ccatgacctg
    41281 gctcaataaa tcccggatcg cctccgggtg gaagggtttg cggatgaagc ccttgatccc
    41341 cagactcaaa gcctcctgga tgacccaatc ttggctatgg gtggtgatca aaaccaccgg
    41401 gattcggcaa tgtttctcgt ttttctgaag ctcccggatc atctccaggc cgttcattcc
    41461 cggcatgtta aagtcagtga ggatcacgtc gatgggatgg gaccccagga gatccagggc
    41521 ctccactccg ttgcccgctt cccagcattc tcccaaagga aggcccgaaa ggtccacaga
    41581 cttgcgaatc accttccgca tggtctggga atcatctacg atgagaacgt tgtaacccat
    41641 ggttctctcc tcatccccgc aaaaggatat tccctccctt ggcccctttt aggggctgtt
    41701 caggagcatt tcggcctctt tgaggcggtc gtccaattcg ctgatgaccc gctccaggtg
    41761 tttctcctgt aaaccaaagc gctgcatgat ttccttatag ccatggtagg acaacccatc
    41821 ggctccccct ccgcagccgg tgaggagggc taccacatcg cacaggtgga ccatcgacac
    41881 caggtcctgg aaatcgggtg cctggaaagg agtatggtga aaccggatgc cggccacgat
    41941 cccgggagcg aattcccact gctcggcaat cctcgccccc agctcggcat gatcgatccc
    42001 caggatttct ttctccgctt ccaggaaaga gattttcctc tccctgacca gggtccggat
    42061 ttcctcgaaa ctttccctca caaactcact gagagccaac tttccaatgt cgtggaggag
    42121 ggcggtagtg aattggagcg gcgatgtttc cttctgaatc tgctgggcca gaatctgcga
    42181 caggatcgcg ctggccaccg agtgtcgccg cagttcccct gcctcgaggt ggtacccctg
    42241 acaggcccgg gtgaagaaag aggcggttcc cttgctgaga acgatctcca tcaactggtt
    42301 gaatccgatc cggactaagg cttcgttcag ggaatgcacc ggcttgcggg gcccgaaata
    42361 ggccgagttg cagacccgga gcacgttggc cgtggttgcc tggtcatact gaatgacctc
    42421 caccacatcc ttggaggagg ttttggggtt ttccaccagt tccagaaccc gatggaccac
    42481 cgcggggaaa gacggcatgt gggtgatgga ctggaggatg aggtcgattt tattcatatc
    42541 tctttctccc cctccccgga agttttcacc gacaccttgc cggtggaaag gtccaggcgc
    42601 acggtacgat tgacctgacc tcccacatct tcggcgtgga tgagaaggtt gcccgtccac
    42661 agaatcttcc gcagggccgc gtagttccgt ttgccgatgt taaaaaaacc ggattcatcc
    42721 agcagctgcg ccccccctgc gatcttgact cggagcctcc gcttgtcccc tccaatcttg
    42781 tagatttcct ggaaaaggcg cgggatgccg gtgtcggcaa acatgaaggg gtttctcaac
    42841 gccttctccg ggttcgtgga agattcgggg agcatgaaat ggagcagtcc tcccgctttg
    42901 accaaggggt cgtagatggt gattcctata caggacccta aagcgaaggt gacaatcatc
    42961 gcctggggat cgtccgagac cttcatgtct cccatcccga caactagatt cataattctt
    43021 ttcgataggt cgcgggttgc acataccgga aggggtgctc gattccagcc agactctccg
    43081 aatgtccgat gaacagaacc cctcctcggc ccaggctttg gaagaaccgg ttcaacaggg
    43141 acgcctgagt cggtttgtcg aaatagatca tcacattgcg gcagaagatc ccgtcaaaag
    43201 gctcggcgaa agaaaagggc tccatcaggt tcaaccgccg gaaaacaaga tatttcttca
    43261 aagaatcctt tacccgcaca tagcccttcc atcggttttc cccacgcaaa aaatattttt
    43321 ttaccaaatc cggggaaatg gtttgaatcc ggccctcggg gtaaattccc gcggcagcca
    43381 ctttcagaac tttgctggaa aggtccgtgg atagcacttg caggtcccag cccctggggc
    43441 tttccagact ctccagcagg gaaatgatga tggaataggg ttcttcgccc gaggaacagc
    43501 cggcgctcca gaaacggagt tttctcccat tccttccttt gtccccgaga acctccggaa
    43561 ggaattcgga tttcaaatat tcgaagtgct gcggctcccg gaagaagctg gtgaaattgg
    43621 tcgacacgga gtccagcaaa tggaccagct cctggcccga agaatcctgt acgacgtgct
    43681 cgtagtactc gcggaacgag ccgaaactcc cggcgcggat gacttttccc aggcgggcct
    43741 tgagcagctc cttctttccc tcggtaagat tgattccgca agcctcgtac accagacggc
    43801 tgatcttttc gaattccccg tcggtcagtt ctgggtttga ccagggatat ccgcccggat
    43861 tcatctgagc ctttcaacgc ggagccgctc gcccctattt aaaacagact tccaccacga
    43921 aatttccacc gttgaggctg aagggaatgg aaaggaccgg ccccttggtg atgtgtttca
    43981 cttcatgtcc ggggcctgat atgaccgaag ggatcgcccc gtgaaaggta tagcccttct
    44041 cctgcaatct cctccgcgaa tccccggaga tcatgttggt cagctctccc acggcatcct
    44101 tcacctcttc ggtgatctct ttctgctctt cccccagcat actggtcatc acctggagga
    44161 tcgcttcccg ggaaaaagtg aggcataggg atccttcggc ctgccccgtg attcccacca
    44221 ctccggatat gtccccgcag gcggcggaac ccttcttgat atagggtttt ccgggcactg
    44281 cctcggcaga ggccatggtc ttcaaaaccg aaagggtggc ttccaaaaag ggattaatga
    44341 gttccgcctt catactcatt ggcttttttc ctcgctcatt gaatttt
//
LOCUS       DASPRR010000515         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1899447, whole genome shotgun sequence.
ACCESSION   DASPRR010000515 DASPRR010000000
VERSION     DASPRR010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899447"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VEL68_19185"
     CDS             <1..1504
                     /locus_tag="VEL68_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HXZ38158.1"
                     /translation="FSEYQVNVLVDNSETKGAPVIIETSPTYRNLFGTIERNVDRMGG
                     MWKTDFTKIKSGSFLRANGGYLVLNALDVFIEPGVWIALKRTLRNRSMEMQSFDPFYL
                     FASSALKPEPIEARVKVVMIGDAYIYETLYYMDEDFKKIFKIKADFDTVMNRNEDSQF
                     QYSTFIRKICQDETLLPFDRTGVAAVIEYGVRLAGRQKKLSTEFHRITDVLREANYWA
                     KKDGSEQVTEKHVDQAIAERIDRLSMIEDKIQEMIDEGIILIDSEGAKVGQVNGLSVF
                     DMGEYAFGKPSRITAKTSMGKAGIINIEREADLSGRTHNKGVLILGGYLRGKYAQDKP
                     LTLTASLAFEQSYSGVDGDSASSTEVYAIISSLANLPLRQDVAITGSVNQNGEIQPIG
                     GVNQKIEGFFDVCRSRGLTGKQGVIIPRQNVGDLMLRKDVVAAVQEGKFHVYPVETID
                     RGIEILTDMPAGEKNPDGTYPPGTVNGLVDQRLRDLAKKMKEFEGGEEKK"
     gene            1627..2025
                     /locus_tag="VEL68_19190"
     CDS             1627..2025
                     /locus_tag="VEL68_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXZ38159.1"
                     /translation="MSDPIDDLKEKTQKTAELYFKGVTGERPFEVWRSFDKELAREIS
                     LFYTGKLYAREKIPHKTRQLVTVAALTVLERTEELKMHLHGAINVGCTPQEVAEVIFQ
                     MATYGGVPVMNSALKAFREVLKEKELWPLK"
     gene            complement(2124..>2421)
                     /locus_tag="VEL68_19195"
     CDS             complement(2124..>2421)
                     /locus_tag="VEL68_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008203565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ38160.1"
                     /translation="KHQFVGDVRGRGLFAGIEFVKDKRTKEPFDPKLKLNGLIGSGAF
                     EKGLITYPGGGGADGIRGDHILLAPPLIISKEQIDELVRILDQTFTEVGKDIAK"
BASE COUNT          569 a          642 c          728 g          482 t
ORIGIN      
        1 cttcagcgaa taccaggtga acgtcctggt ggacaattcg gagaccaagg gggcgccggt
       61 catcatcgag acctccccca cctaccgtaa cctcttcggg accatcgagc ggaatgtgga
      121 ccggatggga gggatgtgga agacggactt tacaaagatc aagagcggct ccttcctccg
      181 ggccaacggg gggtacctgg tcctcaacgc tctggatgtt ttcatcgagc ccggggtctg
      241 gatcgccctg aagcggaccc tgcgcaatcg ctccatggag atgcagtcct tcgacccctt
      301 ctacctcttc gccagctcgg ccctcaaacc ggagcccatc gaggccaggg tcaaagtggt
      361 catgatcggc gatgcctata tttacgagac cctctactac atggatgagg acttcaagaa
      421 gatcttcaaa atcaaagccg acttcgacac ggtgatgaac cgaaacgagg attcccagtt
      481 ccagtactcc accttcatcc gcaagatctg ccaggacgaa accctcctcc ccttcgaccg
      541 aaccggggtg gccgcggtca tcgaatatgg ggtgcggctg gcgggtcggc agaagaaact
      601 ttcaaccgaa ttccaccgaa tcaccgacgt cctgcgggag gccaactatt gggccaagaa
      661 ggacggcagc gagcaagtga cggaaaaaca cgtggatcag gctattgctg agaggatcga
      721 tcgtttgagc atgattgagg acaaaatcca ggagatgatc gacgaaggaa tcatcctgat
      781 cgattccgag ggagccaagg tgggacaggt gaacggcctc tcggttttcg acatggggga
      841 atacgccttc ggcaagcctt ctcggatcac ggccaagacc tccatgggca aggcggggat
      901 catcaacatc gaaagggaag ccgacctttc cgggaggacc cacaacaagg gggtgctgat
      961 cctgggggga tacctgcggg gaaagtacgc tcaggataag cccctgaccc ttaccgccag
     1021 cctggccttc gagcagtctt acagcggggt ggatggggac agcgcatcct ccacggaagt
     1081 ctatgccatc atctccagcc tggccaattt acccttgcgt caggatgtgg ccatcactgg
     1141 gtcggttaat cagaatggcg aaattcagcc catcggcggg gtgaaccaaa agatcgaggg
     1201 gttcttcgat gtctgcaggt cccgcggttt gaccgggaag cagggagtca tcattccccg
     1261 ccagaacgtg ggcgacctga tgttgcgcaa ggacgtcgtg gcggcggtgc aggaagggaa
     1321 gtttcatgtc tacccggttg agaccatcga ccgggggatt gaaattctga ccgatatgcc
     1381 cgccggggaa aagaaccccg atgggactta tcctccaggg acggtgaacg gcctcgtgga
     1441 tcagaggctg cgcgacctgg ccaagaagat gaaggagttt gaggggggag aggagaaaaa
     1501 atagcgttca gccatgagcg ttcagcaaaa gcttttggaa tcaaatactg accgttgaag
     1561 gctggccgtt tgataacatc gtccgggcgc ggggcatcgc gcccacgctg atcgaaaggt
     1621 gggaatatga gcgatccgat agatgacctg aaagaaaaaa ctcagaaaac ggcggagctg
     1681 tattttaaag gcgttaccgg ggaacgccct tttgaagtgt ggcggtcttt tgacaaggaa
     1741 ctggcccggg agatttctct gttctatacg gggaagctct atgcccggga gaagatcccc
     1801 cacaaaaccc ggcaactggt aactgtggct gccctgaccg tgctggagag gacggaggag
     1861 ctgaaaatgc acctccacgg ggcgataaac gtgggctgca cgccccagga agttgccgag
     1921 gtcatctttc agatggctac ttacgggggc gtaccggtga tgaactcggc cctgaaggcc
     1981 ttccgggaag tgctaaagga gaaggagctg tggccgttga aatagacgaa tgaagttgga
     2041 ggttagaggt tggagggtag aggcaaagac tccatctata caggacggta tttccgccca
     2101 taattctcca tggccgcctt ttactatttg gcgatatcct ttcccacctc ggtgaacgtc
     2161 tgatccagga ttctcaccag ctcgtcgatc tgctccttgc tgatgatgag aggcggggcc
     2221 aggagaatgt ggtctccccg gattccatcc gcgccacccc cgccagggta ggtgatcagc
     2281 cctttttcaa aagccccgct cccgatgagg ccgttgagct ttaacttggg gtcgaagggt
     2341 tccttggtcc ttttatcctt tacgaattcg atcccggcga agaggcctcg gccgcgcaca
     2401 tcccctacaa attgatgctt g
//
LOCUS       DASPRR010000516         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2221216, whole genome shotgun sequence.
ACCESSION   DASPRR010000516 DASPRR010000000
VERSION     DASPRR010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221216"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..344)
                     /locus_tag="VEL68_19200"
     CDS             complement(63..344)
                     /locus_tag="VEL68_19200"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ38161.1"
                     /translation="MKNADQWSGLVLLILSGLICWGSLPLPYGNIHNPGPGFYPLWLG
                     ILLGVMSGALILKSVRQKGEQRSLRDILAEKVRWDKVLFVLLALALYGY"
     gene            complement(379..1356)
                     /locus_tag="VEL68_19205"
     CDS             complement(379..1356)
                     /locus_tag="VEL68_19205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ38162.1"
                     /translation="MRGKIFLVLLSLALGISLAPGGVLGAEYPTREIEFIVGFPPGST
                     VDHVGRLISKYAEKYVGKPIIVANKPGGGGSRGFSSLAAAKPDGYTIGLVSQSAILQP
                     YLMKGVTFNYKRSFRIFSEIAYTAEGIYTKKGGPFDIPFPEIVKRAKENPNTIKAGVG
                     GTWTGSDVVRFILEDATGAKFIRIPHPAGGQDLVPGALGGHLDIAIGEGAQWAPLYTA
                     GKFNVLAVSTEKRDPRFPNIPTLKEMGYDAVLAVTFWVGAPAGASDSIVNFWAEVLKK
                     ATAEEGYKQASDQAFNTDSWAGPADSLKAMEKLDDTFQKLIKKYDLKPE"
     gene            complement(1405..2913)
                     /locus_tag="VEL68_19210"
     CDS             complement(1405..2913)
                     /locus_tag="VEL68_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ38163.1"
                     /translation="METLHLIFYGFSVVLQPMNLFCAFLGAVAGTLVGVLPGLGPVGA
                     MSMLLPVTFGMSPTGAMIMLAGIYYGAQYGGSTTSILVNIPGESSSVVTCLDGYQMAR
                     QGSAGKALGIAAFGSFIAGTIGVLGLSFLAPPLVEAALTFGAPEYFALMCLGLTVLTF
                     LAQGSMLKALIMAVLGLFVGTIGIDITTGRQRFTFGSQDLMEGVGLVQIAMGLFGISE
                     ILLNIEKTLKVSVFETRLKGLFPDLQDWRRSMGAILRGTGIGFFLGILPGGGAVIASF
                     ASYAIEKRLSRHPEKFGTGVIEGVAAPESANNAAAQGAFIPLMTLGIPSNVVMALLLG
                     SLMIHGLTPGPLLMTRNPDLFWGVITSMYIGNIMLLILNLPLIPMWVKLLRVPYPLLF
                     PFIILFCLIGVYTINNNAFDIYLMTFFGGVGYLARKFEFETAPFVLAVVLGPMMEKAF
                     RQSLIMFRGDFSVFITRPISGVIFSIAILLLILPLIAKGSKRERIASLKDED"
BASE COUNT          915 a          923 c          917 g          743 t
ORIGIN      
        1 gataaatcga agaaggaaaa ccatgagaag gatggtgatc aagagaaaac caaggatgtt
       61 catcaatatc cgtaaagggc caaagctagg agtacaaaaa ggactttgtc ccagcggacc
      121 ttttcagcca gaatatcccg caaactcctc tgctcccctt tttggcgaac agatttcaga
      181 atgagcgcac ccgacatgac tcccagaagg atcccgagcc atagaggata gaatcctggc
      241 cccggattat ggatattccc ataagggaga ggaagggatc cccagcaaat caacccggat
      301 aggatgagta gaacgagacc gctccactgg tcagcgtttt tcatgaatcc ttttttattc
      361 agcgcgccgt atctgccgct attccggctt taaatcatac ttcttaatca atttctggaa
      421 ggtatcgtct aatttctcca tggctttcag agaatccgcc ggcccggccc aagaatccgt
      481 gttgaaggcc tggtcggatg cctgcttata accctcttcg gcagtcgctt tcttaagtac
      541 ctcggcccaa aaattgacaa tggaatccga cgcccctgcc ggcgccccca cccaaaaggt
      601 gaccgcgaga accgcatcgt atcccatttc tttgagggta ggaatattcg ggaatcgggg
      661 atcgcgcttt tccgtgctca ccgccagaac attgaacttg ccggccgtat agaggggggc
      721 ccattgggct ccttcaccga tggcgatatc caaatgccca cccagagccc cgggcaccaa
      781 atcctgacct cccgcggggt gggggattcg gatgaacttg gcacccgtcg catcctcaag
      841 aatgaaccgg acgacatcgg acccggtcca cgtgcctccc actccggctt tgatggtatt
      901 ggggttttct tttgcccgct ttacgatttc cggaaaagga atatcgaaag gccccccctt
      961 cttagtgtaa atcccctcgg cagtataagc gatttcggag aagatccgaa agcttcgctt
     1021 gtaattgaag gtcacccctt tcatgagata gggttgaaga atggccgatt gggatacaag
     1081 tccgatggtg tatccgtcgg gtttggccgc agccagagaa gagaacccac ggcttcctcc
     1141 ccctccgggc ttgttggcca cgatgatggg tttccccaca tacttttccg catacttgga
     1201 aatgagtcgt cccacatggt caaccgtgct gcccggagga aatcccacga taaattcgat
     1261 ttccctggtg gggtattccg cgcccaagac cccccccgga gccaaagaaa tgcccagagc
     1321 cagcgataac aggacgagaa atatctttcc cctcataccc tttcctcctc tcaatgcttt
     1381 taggagattc ccttacagcc tatcttaatc ttcatccttc agactggcga tcctttcccg
     1441 tttggagcct ttggcaatca agggaaggat cagaagaagg atggcgatac tgaaaatcac
     1501 tcccgaaatg ggccgggtga taaatacaga gaaatcaccc cggaacatga tcagggactg
     1561 acggaacgct ttctccatca tcgggccgag gactacggcc aggacgaagg gggcggtttc
     1621 gaattcaaat ttcctagcca ggtaccccac acctccaaag aaggtcatta aataaatgtc
     1681 gaatgcatta ttgttgatgg tatacacccc gatcaggcag aaaagaatga taaaggggaa
     1741 aagaagggga taggggaccc gcaacaactt cacccacatg gggatcaggg gcaggttcag
     1801 gattaacagc atgatgttcc cgatatacat gctggtaatc acgccccaga agaggtccgg
     1861 attcctggtc atgagcaaag ggcccggagt gagtccatga atcattaagg accccaggag
     1921 caaggccatc acgacattgg aaggaattcc cagggtcatc aggggaataa aagctccctg
     1981 ggccgcggcg ttgttggctg actcgggggc ggccactcct tcgataaccc cggttccgaa
     2041 tttctccggg tgtctggaga ggcgcttttc aatggcataa gaagcaaaag aagcgatgac
     2101 cgctccccct ccgggtagga tacccaaaaa gaatccaatc ccggttccgc gcaggatagc
     2161 gcccatgctg cgcctccaat cttgaaggtc gggaaagagc cctttgagcc tggtttcaaa
     2221 gacgctgacc ttcagggtct tctcaatatt cagaagaatc tcggagatcc caaacagccc
     2281 catggcgatt tggactaatc cgaccccttc catgaggtcc tggcttccga aggtgaagcg
     2341 ctgtcttccc gtagtaatgt cgatgccgat ggtccccacg aagaggccca aaacggccat
     2401 gatcaaagcc ttgagcatgg aaccttgggc cagaaaggtc aggaccgtga ggccgaggca
     2461 catgagggca aagtactccg gagcgccgaa agtgagggcg gcctcgacca gaggaggagc
     2521 taagaaggaa aggcccaaga ctccgatggt cccagcgata aaggaaccga aggccgcaat
     2581 gccgagggcc ttaccggcac ttccctgccg ggccatctgg tagccatcca aacaagtgac
     2641 cactgaagag gactctccag ggatgttcac taagatcgaa gtcgttgagc ctccgtattg
     2701 agccccataa taaatcccgg cgagcatgat catggctccg gtgggggaca tgccgaaggt
     2761 gaccggaagg agcatggaca tggctcccac agggcccaat cccggaagaa ccccgaccag
     2821 cgtgcccgca accgctccca gaaaggcaca aaacaggttc atcggttgaa gaaccacact
     2881 gaagccgtag aagatgagat gaagggtttc cattctcttg ctccaggaat tcccttcggg
     2941 cccccgcgtt cgatacagcc aatttcagga tcggcggggt ctttccagga gttcaaattg
     3001 tataaaatga aacccgcaat ttcaagatga ttcagatgaa tctggacaga gaggtagcaa
     3061 atggttcgtt gggggggatt tgagcattgg aatcattttc ccataaggcc aaaattatct
     3121 ggatatatgg aagctattat tcaatcataa tggattccgg ccttcgccgg aatgacgaca
     3181 aagcgacagg cctctctagc attcgtctct ccggcgaaag ccggagtcca gttcaaggtt
     3241 tataaaaaag aggcttcaac cattgatgtt tagactgacg cggccgcatt ggtaagacca
     3301 aatctgcgcc gcttgaggct ttctcccttg atacacccct cgttatctgc tagactcccc
     3361 tcaatcaaat gctaagagag tcaaaaggca acggcggttt tcaaaaaacc ggtggattgg
     3421 gccgaatatc ggggattttt tgttggggag gggaaaggga atgaaaagct ggaataccgg
     3481 gttagggagg ttatgggt
//
LOCUS       DASPRR010000517         7404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_610829, whole genome shotgun sequence.
ACCESSION   DASPRR010000517 DASPRR010000000
VERSION     DASPRR010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610829"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VEL68_19215"
     CDS             complement(<1..1258)
                     /locus_tag="VEL68_19215"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXZ38164.1"
                     /translation="MKDLNLLKTDILIVGGGGAGMRAAIEAAAHGARVLVADKGPVGR
                     SGTTPMAMEAFQAVGFPGDAEEIHFRDTIEGGYHLGDENLVSILVGDAARRAKDLEAY
                     GVRFKKRPDGAYDPMHHPGQTFPRALFIQGGGFGMLAGLIQEARKHPEVRILNDVFVV
                     QVSLDKGGAASGAIILDLKEGRLKTIQSKAVILATGGYEELWAFNDASCTACGDGIHL
                     AYEAGAELIDLEMLQFYPTVVIHPPSIRGTLFQYELITDPEMLAGRLINGGKEPFFQG
                     KLLRDAVVRAIWREIRDGRGTPHGGVYIDLIHSVKSREDLTRALEKWQPNQFHYLRDM
                     GFDLRETLVEVAPHAHYSLGGVAIDEEARTTVPGLFAAGETTGNLHGANRVSGNALAE
                     TQVFGALAGASAAGWAKGREFDAMPEY"
     gene            complement(1410..2603)
                     /locus_tag="VEL68_19220"
     CDS             complement(1410..2603)
                     /locus_tag="VEL68_19220"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HXZ38165.1"
                     /translation="MRAPLAGLLLLLLGSCVPAVPREDLRGKEPLSFREQEIQGPLGD
                     DASRDSLLASVERSMAYLGKKDIGGGADTISGAKFSPGRISRSLSLFRDILSAARNEA
                     ELDRKVRESFAFYELGRGNPAPSILLTGYFEPLLDGSLVADGEYRYPLYGRPDDLLEQ
                     TQEEGPGEKGTRRIAVRVEGGQAVPYYSRKEIDTDGALQGRGLELVWLRDPWDRFVLH
                     IQGSGQVRLPDGRTIRVGYATSNGRPYRSIGGYLLEQGLLSENEVSMGRIREFLQSNP
                     GREEETLNINERYIFFRILADAEGPSGALAVPLTPGRSVATDLTLFPPGALAYLVSRQ
                     PDLDESGRIIGWKTLRRFVLNQDAGGAIKGPGRVDLFFGSGERAGAAAGEMKEEGKIF
                     FLLAK"
     gene            2838..4019
                     /locus_tag="VEL68_19225"
     CDS             2838..4019
                     /locus_tag="VEL68_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ38166.1"
                     /translation="MKRKKLIPLFIILIAGGALLYYYFFLRPSDGEGTDIAVSGNIEV
                     TTVDVSFKIPGKIEKLAVDEGDPVKQGQVIATLEHRDLVAQKVRAEATLEATQTRIPS
                     LQKNIELQDRSTRDEISQAEAAVQAAQARLQQLLAGSRPQEIQAAKAGVDQAESDLVK
                     RKADRERAEKLFKSNFIAAQDLDAAINAHDVAAANLKKARETYALVVEGPRKEEIDAA
                     RAQYEQAQAALQLARAHRLQLEVLRKDLLTAQAQVKEASTAIAVIDTQIGYTQLLAPL
                     SGVVLVKNAEAGEYVVPGGALVSLGDLERPWLKAFINENDLGRVKLGQKVSVTTDSYP
                     GKIYPGKITFISSEAEFTPKNVQTAKERVKLVYRIKVALENPHGELKPGMPADGRIHT
                     E"
     gene            4126..4434
                     /locus_tag="VEL68_19230"
     CDS             4126..4434
                     /locus_tag="VEL68_19230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXZ38167.1"
                     /translation="MVKEIYLLTKRPEFSKDFAFRDQLRRATISIPTNIAEGYERASR
                     KEYLQFLNIAKGSAGKVRSLLRLAVEIGYIGSPSYENLRNSTLELSRYLSNLMRSLRE
                     "
     gene            4501..5427
                     /locus_tag="VEL68_19235"
     CDS             4501..5427
                     /locus_tag="VEL68_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38168.1"
                     /translation="MLAIQAENLTRTFGPVTAIDRLNLGVNPGEIFGLVGPDGSGKTT
                     LMRLFSGILRPSSGEARILGHPVTSEAERLKERIGYMAQRFALYGDLTVMENIHFYAD
                     LYEVPRKERLPRIDRLLAFSNLAPFQDRLAQNLSGGMKQKLGLACALVHTPEVLLLDE
                     PTNGVDPVSRRDFWRILYDLLQGGVTILISTAYLDEAERCHRVGLMYRGRLLSVGSPR
                     EVKDLMRGELWEVRCPQKMKVLEALKRLPGVKGGGIFGDRIHVLFGEGEKARGEMERL
                     LKESGNEILSLRQIPPTLEDVFISTVPEDTRS"
     gene            5458..6450
                     /locus_tag="VEL68_19240"
     CDS             5458..6450
                     /locus_tag="VEL68_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38169.1"
                     /translation="MESSDSWAVEIQDLVKVFGKFPAVDRVSLRVRKGEIFGFLGPNG
                     AGKSTTIRMLCGILAPTSGQGRVGGFDIYREPEKIKENIGYMSQKFSLYEDLTVEENL
                     DFYSGIYRLPRDTGRQRKEWALQMAGLQDQRKSLTNTLAGGWKQRLALGCAILHEPSI
                     LFLDEPTSGVDPLSRRRFWSLIYEMAGRGITIFVTTHYMDEAEYCDRLALIYRGKVIA
                     LGTPGELKKEHMPEAVWELETEQLVDALNLLKEHVGATTEWPLREVAVFGNTLHLVAP
                     KGAELGTSLPSLLAGKGIPIRRLEQIDPSLEDVFVSLVEEKDREEVRSLGKKDE"
     gene            6578..6982
                     /locus_tag="VEL68_19245"
     CDS             6578..6982
                     /locus_tag="VEL68_19245"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXZ38170.1"
                     /translation="MHLSAAGIVVRSIGETLPQRYPHTRIDAQIIMPNHFHGIITIDV
                     GAIHESPLRNPLVAGIRRKMLLSKMLGYFKMNSAKQINELRGTPGKAVWQRNYYEHVL
                     RNEINQQEIREYIQNNPLKWLEDENHPAKMKS"
     gene            7018..>7404
                     /locus_tag="VEL68_19250"
     CDS             7018..>7404
                     /locus_tag="VEL68_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ38171.1"
                     /translation="MKLIRLKAIARKEFFQIIRDPRSLALAISIPILLLLLFGFALTL
                     DVDRIPTVVYDQDRSPRSRDLIDRFLSSRYFQLEGAVQSYGGLQEMLDTGRALMGLVI
                     PADFSRNLELDRQTTVQVLMDGTDANT"
BASE COUNT         1685 a         2195 c         2056 g         1468 t
ORIGIN      
        1 tgtattcggg cattgcgtca aactctcggc cttttgccca cccggccgcc gatgccccgg
       61 ccagggctcc aaagacctgg gtctcggcca gagcgtttcc cgagacgcgg tttgcgccgt
      121 ggaggttgcc ggtcgtttcc ccggcggcaa aaagcccggg gaccgtggtc cgggcctctt
      181 catcaatagc cacccctccc aggctgtaat gggcgtgggg cgcaacttcc accaaggttt
      241 cccgcaagtc gaaacccatg tctctaagat aatggaattg gttgggctgc cacttttcca
      301 gggctcgggt caaatcttcc cggcttttga ccgaatggat caggtcgatg tacactcccc
      361 catggggcgt tcctcgccca tcccgaattt ccctccagat agcccggact acagcgtccc
      421 ggaggagttt tccttggaag aagggttcct tcccgccgtt tataagccgt ccagccagca
      481 tctcggggtc ggtaatcaat tcatactgaa acagggtccc gcggatggaa gggggatgga
      541 tgactactgt ggggtagaac tggagcatct ccaaatcgat cagttcagcc cctgcttcat
      601 aggccagatg gatcccgtcc ccgcaagccg tgcaggaagc atcattgaag gcccagagct
      661 cttcataccc ccccgttgcc aggatgacgg ctttgctctg gatggtcttc agacggcctt
      721 ctttgaggtc caggattatg gccccggagg cagctcctcc cttgtccaaa ctcacctgaa
      781 ccacaaaaac atcgttcagg attcggacct ccggatgttt ccgggcttcc tgtatcagcc
      841 cggccagcat cccaaaccca cccccctgaa tgaaaagagc gcggggaaaa gtctgtcccg
      901 ggtggtgcat gggatcatag gcgccgtcag gccttttctt aaaccggact ccataggcct
      961 ccaggtcctt tgctctgcgc gccgcatccc ccactaaaat ggagaccagg ttttcatcgc
     1021 ccagatgata acctccctca atcgtgtccc gaaaatgaat ttcttctgca tctccaggaa
     1081 accccaccgc ctgaaaggct tccatggcca tgggagtggt tccggacctc cccacgggtc
     1141 ctttgtcggc cacaaggact cgagcgccat gagcggcagc ttcgatggcc gcccgcattc
     1201 cggccccgcc acctccaaca atcaggatat ctgtcttcag taaattgaga tctttcatgc
     1261 tgcttctttg ccctcagtca aagttatgga aaaatccgaa attcgaagca cgaaatccgg
     1321 acccattcaa aatttcgaat ttcgaaatta ggattttgga tttagaccgg cctcagcttc
     1381 tcaaggagag gcattctgcc aatctgagat tatttggcca acaagaaaaa aatcttccct
     1441 tcttccttca tctcccccgc tgcagctccc gccctttcac cgctgccgaa gaaaaggtcc
     1501 acccgcccgg gacctttgat tgcccctccg gcatcctggt tgagaacgaa acgacgcagc
     1561 gtcttccagc cgatgatcct ccccgattcg tccagatcag gctggcggga tacgaggtac
     1621 gccaaggccc caggcggaaa gagggtcagg tcggtggcga cggaccttcc cggagtcagg
     1681 ggaaccgcca gggcgcccga cggcccttcg gcgtcggcca ggatgcggaa gaagatgtac
     1741 ctttcattga tattgagggt ctcctcctcc cgtccggggt tgctttgcag gaattcccgg
     1801 attcgtccca tggagacctc attctcggac aaaagaccct gttccagcag ataccccccg
     1861 atggaccggt agggtcgccc gttagaggtc gcgtatccta cgcggatcgt cctcccgtca
     1921 ggcaggcgca cctgtcccga cccctggatg tgcagcacga agcggtccca ggggtcgcgg
     1981 agccagacca gttccagtcc cctcccctgc aaggctccat ccgtatcgat ctctttccgg
     2041 gagtaataag ggacggcttg cccgccttcc acccggacgg cgatccggcg tgtgcccttc
     2101 tccccgggac cttcttcctg ggtctgttcc aggaggtcat cgggacggcc gtagagggga
     2161 tagcgatatt ctccgtcagc caccaggctg ccgtccagta gcggctcgaa atatccggtg
     2221 aggaggatgg aaggggccgg atttccgcgg ccaagttcgt aaaaagcaaa actctcccgc
     2281 acctttcggt ccaattcggc ctcattccgc gccgctgaca ggatatcccg aaagaggctc
     2341 agggagcggg aaatgcgccc gggagagaac ttcgctccgg aaattgtatc cgcgcctccg
     2401 ccgatgtcct tcttccccag gtaggccatg ctccgttcca ctgaggccag cagggaatct
     2461 ctggacgcat catctccgag cgggccctgg atctcttgtt cccggaagga gagaggctcc
     2521 tttccccgga ggtcttcccg cgggaccgcg ggaacgcagc ttcccaggag cagcagcaat
     2581 aggccggcta acggcgcgcg caaaccttcc ctcctttttt ccgatattac cattcttggc
     2641 cccaggagga caaacctaaa cccacaggac gcaagtcgcg agaccgcaag gggttaggga
     2701 aagaatccat cacgctctct cgcctttcgc cttgcgacct gcaaccctca aaaaaggctt
     2761 gacataaaaa ggaggaaatt gatcttatag agcagatgcg gttgtcgatg ccgacatccg
     2821 gtagaagccc aaagattatg aaaaggaaaa aactgattcc cctatttatc atcttgatcg
     2881 caggaggagc cctcctctat tactactttt tcctccgccc gtcggacggc gaaggaaccg
     2941 acatcgcagt ctccggcaat atcgaggtca ccaccgtgga cgtttccttc aagatccccg
     3001 ggaagatcga aaagctcgcc gtggacgagg gggacccggt gaaacaggga caggtcatcg
     3061 ccacgctcga acaccgggat ttggtggccc agaaagtcag ggccgaggcc actctcgaag
     3121 cgacccaaac caggattccc tcgctgcaga agaatatcga actccaagac cgatcgaccc
     3181 gcgacgaaat ttcccaggca gaggccgcgg tccaggccgc ccaggcccgg ctccagcagc
     3241 ttctggccgg ctcccggccc caggagattc aagccgccaa ggccggggtg gaccaagccg
     3301 aatccgacct agtgaagaga aaagcggacc gggaacgggc ggagaaactc ttcaagagca
     3361 attttatcgc cgcccaggat ttggacgcgg ccatcaacgc tcatgatgtg gccgcggcca
     3421 acctgaagaa agcccgggag acgtatgcct tggtggtcga ggggccgcgg aaggaagaaa
     3481 tcgacgcggc ccgggcccag tatgaacagg cgcaggcggc cttgcagttg gccagggccc
     3541 accgccttca gctggaggtc ctgcggaaag acctgctcac cgcccaggct caggtcaagg
     3601 aggcttccac ggccattgcg gtgatcgata cccagattgg atacacccaa ttgttagcgc
     3661 ccctgtcggg cgtcgttctg gtcaagaacg cggaggcggg cgagtatgtt gtcccgggcg
     3721 gcgccttggt gagtctgggc gacctggaaa ggccctggct caaggccttt atcaatgaaa
     3781 acgacctcgg ccgggtcaag ctggggcaga aggtctccgt gaccacggac agctatcctg
     3841 gaaagatcta ccccggaaag atcaccttca tctcctccga agccgaattc acccccaaga
     3901 acgtgcagac ggccaaggaa agagtcaaac tggtctaccg catcaaagtg gccctggaaa
     3961 acccccacgg agaactgaag cccggaatgc cggcggatgg acgaatccat acagaatagg
     4021 gcgaaaaggg gaaaggtcga aaaggcgaaa aggaaaaaag aaataagcaa tggaaaaaac
     4081 aatcaagaca tttgaggacc ttctggtttg gcagaagggg atcaattggt aaaggagatt
     4141 tacctattaa cgaaaagacc agaattcagc aaagattttg cttttcgaga tcaactccgc
     4201 agggcgacga tatctatacc gacaaatata gcagaaggat acgagcgggc ctccaggaaa
     4261 gaatatctcc agtttctaaa catcgccaag ggctcagcgg ggaaggtcag aagtcttctc
     4321 cgtctggcag ttgaaattgg ctatataggt tctccaagtt atgaaaatct tcgaaattct
     4381 actctcgaat taagccgata cctatcaaac ctcatgagat ctttgagaga ataagccaac
     4441 aaacatgttt ttgcttcccc tttcgccttt tcgccttttc gccttttcgc ccctgaaatt
     4501 atgttagcca ttcaagcaga gaatctgacc cgaacattcg gtccggtcac cgccatcgac
     4561 cgattgaatc tcggcgtgaa cccgggggag attttcggcc tggtgggccc ggacggatcg
     4621 ggtaagacta ccctgatgcg cctcttcagc ggcatcctcc ggcccagctc cggcgaagcc
     4681 cggatcctgg ggcatccggt taccagcgag gcggaacggc tgaaggaaag aatcggctac
     4741 atggcccagc gcttcgccct ctacggcgac ctgacggtca tggaaaacat ccatttctat
     4801 gccgaccttt acgaggtccc ccgcaaggag cgcctcccgc ggatcgatag actcctggcc
     4861 ttcagcaatt tggccccttt ccaggaccgg ctggcccaaa acctctcggg gggaatgaaa
     4921 cagaaactgg gtctggcctg cgccctggtg cacacccccg aggtcctgtt gctcgacgaa
     4981 cccaccaacg gagtggaccc ggtctcccgg agagacttct ggcggattct ctacgacctc
     5041 ctccagggag gggtcaccat cctgatctcc accgcctacc tggacgaggc ggagcgttgc
     5101 caccgggttg gactcatgta ccggggacgg cttctttcgg tcggctcccc ccgggaagtc
     5161 aaggacctga tgcgggggga gctctgggaa gtgcgttgcc cgcagaagat gaaagttttg
     5221 gaggcgctga aacgccttcc gggagtaaag ggagggggga ttttcggcga ccggattcat
     5281 gtgctctttg gagagggaga gaaggcccgg ggagaaatgg aaaggctgct gaaggaatcg
     5341 gggaacgaga tcctctctct gcgccagatt cctccgaccc tggaagatgt ttttatctcc
     5401 accgtgcccg aagatacgag gtcctaggct taaggcgcag ggctcaggga acgaagaatg
     5461 gaaagcagcg acagttgggc ggtggaaatt caggacctgg taaaggtctt cgggaaattc
     5521 cccgccgtgg accgggtatc cctccgggtc cggaaagggg agatattcgg cttcctcggg
     5581 cccaacgggg cggggaagtc caccaccatc cgcatgctct gcggcatcct cgcccccact
     5641 tctggacagg gacgcgtggg ggggttcgac atctaccggg agccggagaa gatcaaggaa
     5701 aacatcggtt acatgtccca gaaattctcc ctctacgaag acctgaccgt ggaggagaac
     5761 ctggactttt actccggtat ctaccgcctc cccagggaca ccggcagaca gaggaaagaa
     5821 tgggccctgc agatggccgg cctacaggac cagcgcaaaa gcctgacgaa taccctagcc
     5881 gggggttgga aacagcggct ggccctcggt tgcgccatcc ttcacgaacc atccattctc
     5941 ttcctcgacg aaccgacctc gggcgtcgac cccctgtcgc gccgaagatt ctggagcctg
     6001 atctatgaaa tggccggaag ggggatcact attttcgtca cgactcatta catggacgaa
     6061 gccgagtact gcgaccgcct cgccctcatt taccggggca aggtgatcgc cctgggaacg
     6121 cccggagaac tcaaaaagga acacatgccg gaggcggttt gggaattgga gacggaacag
     6181 ttagttgacg cccttaactt actcaaggag catgtagggg cgactactga atggcccctg
     6241 cgggaagtcg ccgttttcgg caacactctt cacctggtag cccccaaggg agccgagctg
     6301 gggacctccc tcccttctct cctcgccggg aagggtattc ccattcggag gctggagcaa
     6361 attgaccctt cgctggaaga tgttttcgtt tctttagtgg aggagaaaga ccgggaagag
     6421 gtgcgatcat tgggcaagaa agatgaatga gcccccaaat catcatcgcc ggtctgttcg
     6481 gttgcggcat tacgattatg gccaaccagg ggcgtatttt ctgacggttt gcacgcatgg
     6541 gcgcgattgt ttgttcgggg agattgtgga gggggaaatg catttaagcg cggcggggat
     6601 agtagtgcgg tccatagggg aaacattgcc gcaacgatac ccccatacaa gaattgatgc
     6661 ccagataatc atgcccaatc atttccatgg gatcatcacg atcgatgtag gggcgattca
     6721 tgaatcgccc ctacgaaatc ccttggtcgc cggtattcgt cggaaaatgc tcctatccaa
     6781 gatgctcggc tatttcaaaa tgaattctgc caaacaaatc aacgagcttc gcggcacccc
     6841 gggcaaagcc gtctggcaac ggaactacta cgaacacgtc ctccgcaatg aaatcaacca
     6901 gcaagaaata agggaataca ttcaaaacaa ccctctgaag tggctggaag atgaaaacca
     6961 tccagccaag atgaaatcct aggggcgatt catgaatcgc ccctactccg cgacaacatg
     7021 aaactcattc gtttaaaagc catcgcccgc aaggaattct tccagatcat ccgcgacccg
     7081 cggtctctgg ccctggcgat ttccattccc attctccttc tcctcctctt cggctttgcc
     7141 ctcaccctcg acgtggaccg gatccccacc gtggtctatg accaggaccg ttcgcccagg
     7201 tcccgggacc tcatagaccg tttcctgagc tcccggtatt ttcagctcga aggcgcagtg
     7261 cagagctacg gcggccttca ggagatgctg gataccggcc gggcgctcat ggggctggta
     7321 atcccggcag acttttcccg gaacctggaa ttggaccggc aaaccaccgt ccaggtgctc
     7381 atggacggga cggacgccaa cacc
//
LOCUS       DASPRR010000518         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_272575, whole genome shotgun sequence.
ACCESSION   DASPRR010000518 DASPRR010000000
VERSION     DASPRR010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272575"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VEL68_19255"
     CDS             complement(<1..192)
                     /locus_tag="VEL68_19255"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXZ38172.1"
                     /translation="MFTGLIQDVGKVQAVEPAGGGVRLTVSTRLDIGLFKVGDSISVD
                     GVCLTVVRVSGTTFTVEVSP"
     gene            complement(308..1411)
                     /gene="ribD"
                     /locus_tag="VEL68_19260"
     CDS             complement(308..1411)
                     /gene="ribD"
                     /locus_tag="VEL68_19260"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXZ38173.1"
                     /translation="MDRDEKYMRMALGLARKGRGRTTPNPMVGAVVVRGKAVVGKGYH
                     RRAGEPHAEVLALRQAGKKAKGATLYLNLEPCDHFGKTPPCTQAILKAGIQRVVAGMR
                     DPNPLVSGRGFRRLKRAGVRVDVGLLEEECRELNAPFGKFISTGNPFVILKAAVSLDG
                     KVATKSGDSRWISGGASRKYVHRLRSDVDAVMVGVGTVRRDDPLLNVRLAGRKNPRHP
                     LRIIVDSRLRIPADSRIVRTAAEFPTLVAATPSAPLKRVDWLKKKRVEVLVVRRNRQG
                     RVSLLALLKELGSRGIQSILLEGGPTLNASAWEEKIVDRVLLFIAPKVIGGRETSGMI
                     GGEGVGKVKNARRVEILRVRRMGPDLLIEAKPL"
     gene            complement(1496..1966)
                     /gene="nrdR"
                     /locus_tag="VEL68_19265"
     CDS             complement(1496..1966)
                     /gene="nrdR"
                     /locus_tag="VEL68_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXZ38174.1"
                     /translation="MRCPFCQHMDDKVIDSRLSKEGEMIRRRRECTHCLRRFTTYERV
                     EETLPLVIKKDGRRENFDRTKILAGLHKACEKRPISVEVLEKLVDRIEQKLQESGERE
                     VHSRVVGEQIMQELQSLDEVAYVRFASVYRSFKDVNEFMSEVKELLEKGQKKKE"
     gene            complement(2272..2775)
                     /locus_tag="VEL68_19270"
     CDS             complement(2272..2775)
                     /locus_tag="VEL68_19270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXZ38175.1"
                     /translation="MTVGRFYPTKREAGLPGQGHPVMSEKGKESKSQYGIVNFERRRS
                     PRFSVDLPIEYSRVDSSRKNNGHTGNASGGGLMLFLGEPMEVGAGLRIKVFFSSEPKL
                     DSIEVQAQVVWTEIPFGKEKDHRCGVRFTDIASEDLTKLKNFLDGLSTIRPPFRIPSS
                     SQQDFKP"
     gene            complement(2857..3306)
                     /locus_tag="VEL68_19275"
     CDS             complement(2857..3306)
                     /locus_tag="VEL68_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HXZ38176.1"
                     /translation="MNRPTWEEYFMEITRLVAKRATCLRRQVGAVLVMDKRLLATGYN
                     GAPSGLAHCLEVGCLREKRQIPSGERHELCRGLHAEQNVIIQAAFHGIRIQGSTLYCT
                     NLPCAICTKMLINAGIQEIIYEGGYGDDLSKEMLLEARIPIRQLSLK"
     gene            complement(3303..>3457)
                     /locus_tag="VEL68_19280"
     CDS             complement(3303..>3457)
                     /locus_tag="VEL68_19280"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HXZ38177.1"
                     /translation="ANLLALGGRLIGKGLAREILKVWLETEFQGGRHQKRLDKITGLE
                     EKAGKK"
BASE COUNT          679 a         1081 c          902 g          795 t
ORIGIN      
        1 gggggatact tccacggtga atgttgtgcc ggacactctg accacggtca ggcatactcc
       61 gtccacggag atactgtctc cgaccttaaa taggccaatg tccagccgag tggaaacggt
      121 cagccggacc cctccgcccg caggttcaac agcctggacc ttgcccacat cctggatcaa
      181 accggtgaac atattaactc gaccattgat tcagctataa agaaagggcg agtcgtcacg
      241 cctttggcgg aatcacccct acggtcaaat tttatcacgc tcctgatttc cttggcaaag
      301 tctatcccta aaggggcttt gcctcgatca ataggtccgg tcccatcctc cgaactctca
      361 ggatctccac tcttcgggca ttcttcactt tccccactcc ttctccccct atcattcccg
      421 atgtctccct cccgccgatt accttgggcg cgataaagag caagacccgg tccacgattt
      481 tttcttccca cgcgctggcg ttgagggtag ggcccccctc caggaggatc gactgaatcc
      541 cccgggaacc cagttccttc agcaaggcta gcaagctcac ccgcccctgc cgattcctcc
      601 ggacgaccag gacctccacc cgttttttct tcaaccaatc aacccttttc aggggggccg
      661 atggagtggc tgccaccagg gtggggaatt cagcggcggt tcgaacgatg cgggaatccg
      721 cagggattcg cagccggctg tccacaatga tccgcagggg atgacgggga ttctttcgtc
      781 ccgcaaggcg gacattcagc agagggtcgt ccctgcgcac cgtcccaacc cccaccatca
      841 ccgcatccac atccgaccgc aaacggtgta cgtactttcg cgaggctccg ccggagatcc
      901 agcgcgaatc gccggacttg gtggccactt ttccgtccag gctgaccgcc gccttcagaa
      961 tgacgaaagg gttcccggtg ctgataaact tgccgaaggg ggcatttaac tcccggcact
     1021 cctcttcgag aagccccaca tccaccctca cccccgccct tttcagccgg cggaatccgc
     1081 gccccgacac caggggattg gggtccctca tgcccgctac aacccgctgg atgcccgctt
     1141 tcagtatggc ttgggtgcag ggcggcgttt tcccgaagtg gtcgcaaggc tcaaggttga
     1201 ggtacagggt tgcgcctttc gccttcttcc cggcctggcg cagggccagg acttcggcgt
     1261 ggggctcacc cgcgcggcga tgataccctt ttcccacgac ggccttgcct cgaaccacca
     1321 ctgccccgac catcggattc ggggttgtcc ttcccctccc cttgcgggcg agacccaggg
     1381 ccatccgcat gtatttttca tctctgtcca taaaaaaatg gaggggaaag ggaagaccct
     1441 ccgcgtttcc ccttcccctt ttcgtccttt caccccgcgg atttttctcc ggcggtcatt
     1501 ccttcttctt ctgtcccttc tccagcagtt ccttcacttc gctcatgaat tcgttcacgt
     1561 ctttgaacga acggtaaacc gaagcaaacc gcacataggc aacctcgtcc agactctgca
     1621 gctcttgcat gatctgctcc ccgaccaccc ggctgtggac ttccctttcc ccgctttcct
     1681 ggagtttctg ctcgatgcga tcgactagct tttccagaac ctcgacgctg atggggcgct
     1741 tctcgcaggc tttgtggaga ccggccaaga tctttgtgcg gtcgaaattc tcccggcggc
     1801 catccttctt gatcaccagg gggagggttt cttccacccg ttcgtaggtc gtgaaccggc
     1861 gcaggcaatg ggtgcattcc ctccggcgtc ggatcatttc cccctccttg ctcaggcggg
     1921 aatcgatgac tttatcgtcc atgtgctggc agaatgggca gcgcaatttt tacctcttta
     1981 aagagaaagg ttgtttccgg tgacaggtaa tcaataccat aagccatcgt ttccttcaag
     2041 aacccattcc tgggaaatga ccgaggaatc ctgttcccgg gcctgcagaa cggactccag
     2101 tagggatcgg ctctccccgg atgccagggc tgggactttt gactcatttc ccaggaaggg
     2161 gcgccacctc ggatttccct cccctcaatc cgtgtccaaa gaagagaagc atggctccgg
     2221 ttgagcggca attttactct aaggaaatta cggggcagga gaaaagggga gctaaggctt
     2281 gaaatcttgc tgcgaactcg acggaatccg gaaagggggc cggatcgtgg agagaccatc
     2341 cagaaaattt ttcaacttgg ttaagtcctc ggaagcgata tccgtgaacc tgaccccgca
     2401 ccgatgatcc ttttcttttc cgaaggggat ctcggtccag acaacctggg cctggacttc
     2461 gatggagtcc aatttgggtt cggaagaaaa aaagacctta atgcgcaggc ccgcccccac
     2521 ctccatcggt tcgcccagga agagcatgag gcccccgccg ctggcgtttc ctgtatgacc
     2581 gttattcttg cgcgaggaat cgactcggga atactcgatg ggcaggtcca ccgagaatcg
     2641 ggggctccgc ctcctttcga aattaacgat cccgtattgg gacttggatt cttttccttt
     2701 ttcgctcatg accggatggc cttggccggg aagcccggcc tccctcttgg ttggataaaa
     2761 cctccccacg gtcattaaac ctccaatgtt aggaaaagtc aagccttgac aaaaggcgtg
     2821 attattcctc aaggaggaag gttcttctgg aagggcctat ttcagggata gctggcggat
     2881 ggggatgcgg gcttccagga gcatctcttt ggacagatcg tctccatagc cgccttcgta
     2941 gatgatctct tggatccctg cgttaatcag cattttggtg cagatggcac agggcaggtt
     3001 ggtgcaatag agggtcgaac cctgaatgcg gattccatga aaggccgctt ggataatgac
     3061 gttctgctcg gcatgcaagc cccggcagag ttcatggcgc tcaccggagg ggatctgtct
     3121 cttctcccgc aggcacccca cctcgaggca atgggccagg cctgagggag ccccgttgta
     3181 tccagtagcc aataggcgct tgtccatgac caggaccgcc cctacttggc gccgtagaca
     3241 ggtagcccgc ttggccacca accgggtaat ttccatgaaa tattcttccc aggtgggtcg
     3301 gttcatttct ttccggcctt ctcttccagg ccggtgatct tgtcgagccg cttctggtgc
     3361 cggccgccct ggaattcagt ctccagccag accttcagaa tctccctcgc caatcctttc
     3421 ccgatcagcc gtccccccaa agccaggagg ttggcgt
//
LOCUS       DASPRR010000519        18546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2221335, whole genome shotgun sequence.
ACCESSION   DASPRR010000519 DASPRR010000000
VERSION     DASPRR010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 18546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18546)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18546
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221335"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..86)
                     /locus_tag="VEL68_19285"
     CDS             complement(<1..86)
                     /locus_tag="VEL68_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXZ38178.1"
                     /translation="MSTVTMKQLLEAGVHFGHQTKRWNPKMK"
     gene            complement(282..1487)
                     /gene="argJ"
                     /locus_tag="VEL68_19290"
     CDS             complement(282..1487)
                     /gene="argJ"
                     /locus_tag="VEL68_19290"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXZ38179.1"
                     /translation="MTEHIPPDFHVPGFLAAGIAAGIKKTQMKDFALIYSEVPAVAAG
                     VFTTNRVKAAPVLISRERVRSGKGRAILVNSGCANACTGKKGIGDGQHLSRLLASSLK
                     IDPREVFLASTGVIGKPLPLDIMEERLPSLVASLSYEGLADAAEAIMTTDTKPKAVIS
                     RGRVNGEEVTLAGIAKGAGMISPRMATLLVFILTDGDISAGALRKALKEGVGESFNRI
                     TVDGDMSTNDTLLILANGQAGNRRILPATPGFKRFSALLRELLLALAKKTVADGEGVT
                     KVVRILVERARTASEAEKVARAVANSPLVKTAFFGEDANWGRILCAAGYSGALLDPDR
                     VDIFFDDVHVVRQGQGMGPEKEDPATAVMKKGEYTVRINLHRGKKGTFLFTTDFSFDY
                     VKINASYRS"
     gene            complement(1573..4545)
                     /gene="secA"
                     /locus_tag="VEL68_19295"
     CDS             complement(1573..4545)
                     /gene="secA"
                     /locus_tag="VEL68_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXZ38180.1"
                     /translation="MLFEALRKVFGSKNEREINRLYPLVQAINELEPEVLKLTDAQLK
                     AKTPEFRERLAKGEALETLLPEAFAVVREASRRTLGQRHFDVQLIGGMVLHQGKIAEM
                     KTGEGKTLAATLPVYLNALTGQGVHVVTVNDYLAKRDAAWMGSIYHFLGMSVGVIVHE
                     LDDAQRQQAYRCDITYGTNNEFGFDYLRDNMKFRLEDMVQRELNYAIVDEVDSILIDE
                     ARTPLIISGPVERVLEDGYYEELKPLVTRLRQSQSRLMDGVLQEALKVLKEEPESEEA
                     IEKLLLVKRGDPKHPGFLDLIAREPALKTKIDRLESILSGQKTLSQFLGKLYCSIDER
                     SNAVEMTEKGMELLSKGQPDAFVVPSLDEGYVEIDSNPTLSPSEKVQAKEALETRYRQ
                     SSERIHTVHQLVKAYWLFEKDVNYVIKDGQVIIVDEFTGRMMPGRRWSDGLHQAIESK
                     EGVKVAEENQTLATITFQNYFRMFKKLAGMTGTADTEAAEFQKIYKLDVVVIPTNMPM
                     IREDFSDVIYRTQREKYQAVVEEIKDCYRRGQPVLMGTITIENSEKLSQMLRADKVPH
                     QVLNAKYHEMEANIIAQAGRYKSVTISTNMAGRGTDIMLGGNPEGLAREEARKRKIDP
                     AADPAGWQKILEGMRGLTSQEYQKVIEAGGLHVLGTERHESRRIDNQLRGRSGRQGDP
                     GTSRFYLSLEDDLLRIFGSQRISGVMSRLGMEEGEPIEHSLITRAIENAQKRVEAHNF
                     EIRKHLLEYDDVMNKQREVIYGLRKQVLAREGLPEEVQETIEELAESLVDSIADAKSY
                     AEEWDYRRLSESVMRLFAFVPEIRSEETAEMTREKLLEKVLARAKEVYRKKEVEFGAE
                     TLRYLEQVIYLQTIDTLWKEHLLAMDHLKEGIGLRGYGQRNPLQEYQKEGYEMFTELV
                     SSIKEETIQKLFRVQIARPEEVTRFEKVQKKPMVLSHGAQEVEEKAQPVKRESKKIGR
                     NDPCPCGSGKKYKKCHGR"
     gene            complement(4806..5786)
                     /locus_tag="VEL68_19300"
     CDS             complement(4806..5786)
                     /locus_tag="VEL68_19300"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXZ38181.1"
                     /translation="MAKKKYTVLVFSQQASRVKRFIFSPLTLKIGAAVLTLLLVVSAY
                     LFYDYITYQKNIFDLKELRNELNSRQAEIQSFLEKISLLEEQLTRLKLVEEQVKKDLK
                     EVQELKNEKKVKKLTPLPSVPQKTSENKQDLPPHQKALLLRQDEVSILERERPKLVSR
                     LYMELMDLSKEAFQRERTLKDLKEFLQAQKSVLLSIPSLWPVYGRITSSFGEVRFSPS
                     SGGTRPHMGIDISAPTGTPIVAPADGVVLSAGREAEYGLLICVDHGHGFTTMYGHLKE
                     IRVKPGDRVKTGQLLGTVGTTGNTTGPHLHYEVRIHGRPANPFSYLTQTS"
     gene            complement(6051..6509)
                     /locus_tag="VEL68_19305"
     CDS             complement(6051..6509)
                     /locus_tag="VEL68_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXZ38182.1"
                     /translation="MPIRKAKISDVRDIQKLIERSAKKGEMLPRSLSELYDNLRDHYV
                     YVEDGLEGIIGTCAMHICWEDLAEVRSLVVQEEYQHQGIGSKLIEACLSEAVSLGLYR
                     IFALTYRPDFFKNHGFKVVDKAALPHKIWADCIKCVKFPECDEIAVLLEV"
     gene            complement(6658..8349)
                     /gene="recN"
                     /locus_tag="VEL68_19310"
     CDS             complement(6658..8349)
                     /gene="recN"
                     /locus_tag="VEL68_19310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HXZ38183.1"
                     /translation="MLSDLTIRNFAIIEQIHARFGPGLNTLTGETGAGKSILVDALNL
                     LLGTRASSEMIRTGREEASVEAFFDLEEGPKVTLVKNLGLKESEGLQVRRLIHHSGKS
                     RAFLNGNPITLHMLEGLGEELIHIYGQHEHQHFLDPLRHIDILDSTGGLLDQRARFQE
                     VYARWAKAASELSDLTSRQKQRLERIEFLAFQSGEITRANPKPGEDEELAGERSRLVH
                     SEKLHSIAHFGTEALYGESGSVAERLKSTLQRLKEGAKVDSTLGPLVTSVESILFQSE
                     DVASALRAYREKVYFDPKRLEAIESRLDELNRLKKKYGPSLADVSAYKEKIDGERKSL
                     GSLEESISQWERTAAQELARAAELARELSGKRKGIARDLGARLEKELSTLGMKKVRFR
                     IEVHPNEGNEADGGPRPPLNEKGMDQVEFLLSPNPGEELRPLAKIASGGELSRIMLAM
                     KRIFAEESSVKTLIFDEVDAGIGGGIAEIVGRKLKEISRQHQVFCITHLPQIACFADS
                     HYRVSKKESGGRTFVEVKELSDEERLEEIARMLGGVRITDKTLEHAREMLKNAKK"
     gene            complement(8372..9229)
                     /locus_tag="VEL68_19315"
     CDS             complement(8372..9229)
                     /locus_tag="VEL68_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXZ38184.1"
                     /translation="MLNRIGIVVKPDKPEAISVARELTGWLGRKNIRVALDPSVASSL
                     NPALACPREKIGLEVDLLVVLGGDGTLLSAARHLGGEKVPILGVNLGGLGFLTEITLE
                     ELYPVLERTLEGKVETERRMRLNVAVIRQGEKVGEYTILNDAVISKTVLARIIHLRNS
                     VDGAYVTTYRGDGVIICTPTGSTAYSLAAGGPIVYPSLDSVLITPICPHTLTNRPLLI
                     PDRATVEFTLETEESDVRLTLDGQIGCNLLPYDRICITKAQNHVLFVKSPYRDYFEIL
                     RTKLKWGER"
     gene            9898..11235
                     /locus_tag="VEL68_19320"
     CDS             9898..11235
                     /locus_tag="VEL68_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXZ38185.1"
                     /translation="MELFEASRPKLEERRDLAPLADRVRPERLEDFVGQEHLLGPKRF
                     LRSLIEKDQLQSLIFWGPPGTGKTTLASIIARSTRSHFVAFSAVLSGVKEIRQVVQEA
                     EEHKARGRRTIFFVDEIHRFNKAQQDAFLPHVERGTIILIGATTENPSFEVNSALLSR
                     CKVLTLRSLTEEEIGRILTRVLSDEDKGLGKLRVELAPEAVRFLTEMAHGDARIALNA
                     LEAAALTATPDARGLRALTLPMVEEAMQRKALLYDKEGEEHYNVISAFHKSLRGSDPD
                     AALYWLARMIEAGEDPLYVARRMVRFASEDVGMADPQALGVALWSMEAFHFVGLPEGE
                     LALAEAAVYLATAPKSNAIYTAYESAKGDVAKKGALPVPFPIRNAPTALMKELGYGRG
                     YRYAHDYPEGLVEQEYFPKELGNRIYYRPMERGFEKIIGQRLEAWRKKMGRKL"
     gene            11289..11387
                     /locus_tag="VEL68_19325"
     CDS             11289..11387
                     /locus_tag="VEL68_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aurora kinase A-interacting protein"
                     /protein_id="HXZ38186.1"
                     /translation="MGSVVKKRRKKISKHKHRKLLKRTRVQRRKRK"
     gene            complement(11423..11728)
                     /locus_tag="VEL68_19330"
     CDS             complement(11423..11728)
                     /locus_tag="VEL68_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXZ38187.1"
                     /translation="MTKFQLIQKLMEKSNNLAQQDAKTIVNTIFSSMIDALVKGERIE
                     IRGFGNFTVRNYQSYLGRNPRTGTKVDVSPKRLPFFKVGKELKERVDILPPPAPTPE"
     gene            complement(11881..12132)
                     /locus_tag="VEL68_19335"
     CDS             complement(11881..12132)
                     /locus_tag="VEL68_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38188.1"
                     /translation="MVKQIERQIRDFQRELAEIKKEQSGLRLQPCKGDAEIRAKEGKL
                     EDLDKRARSLRESIRELERKNQEQISEPCRKEGYESPFV"
     gene            12418..13131
                     /locus_tag="VEL68_19340"
     CDS             12418..13131
                     /locus_tag="VEL68_19340"
                     /inference="COORDINATES: protein motif:HMM:NF017459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNA1162 family protein"
                     /protein_id="HXZ38189.1"
                     /translation="MNQSIPKMLFWASIFLLLAGCATPLSDVSQDFKSQRPTRIAVLP
                     AKNETADMDGPIVFRLLAGAELADKGYALVDFARIDEALREKGIEEAGQIESLTSQEI
                     GESVGADGLLYVTVLTYGRQVGVHLKMEGSFILVDAKTGQKLWFSELSVSDDVVLEGG
                     TAVLIGELFGGQDKRKSREKAMETYLAMRQARIAQAVNRFRAHPIKREVFRVITTDMD
                     KIYLLEIFFRRNFRSLPRP"
     gene            13362..13985
                     /locus_tag="VEL68_19345"
     CDS             13362..13985
                     /locus_tag="VEL68_19345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXZ38190.1"
                     /translation="MINLSDQSLIEKVRGGDYQAFEFLVTRYEAKVYRLAIRMLRNPQ
                     DAEDALQETFLQVFRGLSGFEGRSQFSTWLFRLATNVCLMRIRHRETEPSKLLPLEDY
                     LPKLEEGEGPPMVDWADRPEDALLSKESREQMMGALDKLPPEYRAVFILRDIEGFSNA
                     ETGESLGISTAAVKSRLHRARLALRGMLSEYFQRKQDFGGLETRLED"
     gene            13989..14267
                     /locus_tag="VEL68_19350"
     CDS             13989..14267
                     /locus_tag="VEL68_19350"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXZ38191.1"
                     /translation="MEKDEKTTQVFSCKGMECKEMLEKLSEYIDGELDPKICQDLEKH
                     MADCNPCLIFVNTLKKTISLFKYASSEPLPKEVHIRLHDYLKKNCQGC"
     gene            14436..14780
                     /locus_tag="VEL68_19355"
     CDS             14436..14780
                     /locus_tag="VEL68_19355"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXZ38192.1"
                     /translation="MKIQKMKARSKVWIEVGGEVVAGDGKVDLLERIAETGSIQKAAQ
                     EIGMSYRHAWGFLQKMEKRGGIKLVETQVGGREGGGAKLTPPGKEFLRRYSAFREGLD
                     KYIEDKFKRAFK"
     gene            14872..16002
                     /locus_tag="VEL68_19360"
     CDS             14872..16002
                     /locus_tag="VEL68_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HXZ38193.1"
                     /translation="MRFLGNEYNRNELAGAFGDLGTFIPFVAAYITINKMDPLGILVA
                     FGVFKIFVGQYFKTPMPVQPMKAIGGMAIAHPESITQGMIWGSGLFTAVFWLILGLSG
                     AVSWLYKITAKPITRGIMLGLGLSFVLQGVGMMKEQPWVALIAAAMTFFLLSQEKIPA
                     MLLLLAFGMGVAFMANPSLWQDLSQISVSFRFPELSLGRMSWSDLVAGTLILGLPQAP
                     LTLGNAIIGTAEENNELFPDRPAKVKTIAVDHGIMNVFSTAIGGIPLCHGAGGMAGHV
                     RFGARTGGALIILGIIVLLLGLFFSDSVATIFKMFPPSILGVILFFTGLELASITRDI
                     GNQRSDVYIMLVVAGLAMWNMGAAYLAGLVLYYGVEKKVLKF"
     gene            complement(16010..16744)
                     /locus_tag="VEL68_19365"
     CDS             complement(16010..16744)
                     /locus_tag="VEL68_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXZ38194.1"
                     /translation="MKRFVEILSTRREFIKKGGILMASTAILQTDGASTLAASKSKDK
                     KYEGKKGEEISPAEDLMREHGVLSRILLIYDDISRRLEKDIKYPPEVLTGAAVLIRRF
                     VEDYHEKLEEDHVFPRFVKVGKLVDLVNVLLEQHQAGRRLTEKIKNLGSLSTLNPDEK
                     RGLLKNLGLFARMYRPHKSREDTVLFPAFQSVVSAKEYDSLGEKFEEQEDKLFGEHGF
                     EKVVEEVAGLEKSLGIYELSHFTAKG"
     gene            17014..17379
                     /locus_tag="VEL68_19370"
     CDS             17014..17379
                     /locus_tag="VEL68_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38195.1"
                     /translation="MKCDRCDKTLESGNERNHLGRNLCEDCYMDALSPSRACDPWAVH
                     SAKSFERQSGGTGALTPVQSEILCLLRETGGMEPPVLLRKLGGRLTLKELEREFATLR
                     HMEKARGEKRGDRIVWRLW"
     gene            17597..18046
                     /locus_tag="VEL68_19375"
     CDS             17597..18046
                     /locus_tag="VEL68_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXZ38196.1"
                     /translation="MKEEEKIAQLKKMVNQLIKVEEEVIALTDKTAGQAANSTVKYLL
                     RAIQHDSRKHADLGRAALEILEYKQVAGAEERKEVWDLLKKHAELERKHQDFLSEMKK
                     LTDTPALKYIFEQVWNDEKIHHAMLETMMTKRFEKDPAKYWGALLGS"
     gene            complement(18158..>18546)
                     /locus_tag="VEL68_19380"
     CDS             complement(18158..>18546)
                     /locus_tag="VEL68_19380"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXZ38197.1"
                     /translation="GIAMGQVTPGPIMITATFLGYKLSGFWGAAMTTLAIFSPAFYIV
                     TLLIPHYDKVRGIAAVRIFEVGILASFVGMLGLVLYNFGRATFVDIPSILFTIGAFIA
                     LLKKIDLSYILLTGTILSLLLFGFLL"
BASE COUNT         4059 a         4964 c         5128 g         4395 t
ORIGIN      
        1 tccttcatct tgggattcca cctcttggtt tggtggccaa aatgaactcc ggcttccagc
       61 aactgcttca tggtgacggt ggacatagat ttttcctcct gggtttttcc tccacttccc
      121 ttttcccggc cgttgatccg agaaagcagg acacccaagg ccggtcggga agtgtgtgtt
      181 ttttaaatct ctattatacc aattctttcg atgaccgcaa ccagaaaagc taatttagtt
      241 cggagcaacg gccgctccga actcatttac tccgaactgc ttcaactccg gtacgaagcg
      301 ttgatcttga catagtcaaa agaaaaatca gtggtgaaga ggaaagtccc tttctttccc
      361 cggtggaggt tgatgcggac cgtatactct cctttcttca tgactgctgt ggccgggtct
      421 tctttctcgg gacccattcc ctgtccctgg cggaccacat gaacatcatc gaaaaagata
      481 tccaccctat ccggatcaag gagggctccc gaataaccgg cggcacagag gatccggccc
      541 caattggcgt cttccccgaa gaacgcggtt ttgaccaggg gggagttggc aacggccctg
      601 gccacctttt cagcctcgga tgccgtgcgg gccctttcca ccagaatccg gaccactttg
      661 gtcaccccct cgccgtcagc aacagtcttt ttggccagcg ccaaaaggag ttcacgcaga
      721 agggcggaga acctcttgaa cccgggagtg gcaggaagaa tccggcggtt tcccgcctgc
      781 ccgttggcca ggatcaggag agtgtcgttg gtgctcatgt ccccgtccac ggtgatccgg
      841 ttgaaggact ccccgacccc ttccttcaga gctttccgca gcgctccggc tgagatgtcg
      901 ccgtcggtaa ggataaaaac cagcagggta gccattcggg gagagatcat cccggctccc
      961 ttggcgatcc cggccagagt cacctcctcc ccattgaccc taccccggct gatcaccgct
     1021 ttcggcttcg tgtccgtagt catgatagcc tcggctgcat cagcgaggcc ctcgtaggaa
     1081 agggaagcca ccaaggaggg gaggcgctcc tccatgatat ccaggggcaa gggttttccg
     1141 atcaccccgg tggaggccag gaaaacctcc cgggggtcga tcttcaagga agatgcgagg
     1201 aggcgggaga gatgctggcc atcgccaatc cctttctttc ctgtacaggc gttggcgcag
     1261 ccggagttga ccagaatggc ccgtcccttc ccggacctga cccgttcccg ggagatgagt
     1321 accggagcgg cttttacccg gttggtggta aagaccccgg cggcgaccgc cggaacctcc
     1381 gaatagataa gggcgaaatc cttcatctgg gttttcttga tccctgcggc gatgccggca
     1441 gccagaaatc cggggacgtg gaagtcgggg gggatgtgct cggtcatgac cactccttaa
     1501 cggcgcaaag agcagagagt ataaagaatc gagttgctgg ctctacgctc caggccctat
     1561 gcgctatgcg ttttatctgc cgtggcactt tttgtatttc ttcccgctgc cgcaggggca
     1621 ggggtcattg cgtccgatct ttttgctctc ccttttcacg ggctgggcct tctcttccac
     1681 ctcttgggcc ccgtgactca ggaccatggg cttcttctga accttttcaa agcgggtcac
     1741 ctcttcgggg cgggcgatct gtacccggaa gagtttctgg atcgtctcct ccttgatgct
     1801 ggagacgagc tcggtgaaca tctcgtaccc ttccttttgg tattcttgga ggggattgcg
     1861 ttgcccgtag ccccgcaggc cgattccctc cttgagatgg tccatggcca gcaggtgctc
     1921 tttccacagg gtatcgatgg tctggaggta gatcacctgc tccaagtagc ggagggtctc
     1981 ggcgccgaac tcaacctcct ttttccgata tacctccttt gctcgggcca ggaccttctc
     2041 caggagcttt tcccgggtca tctccgcggt ctcctccgaa cgaatctcgg gaacgaaagc
     2101 gaagagacgc ataaccgatt cactcagccg gcgatagtcc cattcttcgg cgtaggactt
     2161 ggcgtcggcg atagaatcca cgaggctctc ggccagctcc tcgatggttt cctggacctc
     2221 ctcgggaagc ccctcccgcg ccaggacctg tttcctcagg ccgtagatca cctcccgctg
     2281 tttgttcatc acatcgtcgt attccaggag gtgcttgcgg atctcgaagt tatgggcctc
     2341 gacccgtttc tgggcgtttt cgatggcccg ggtgatgagg gaatgctcga tgggctcccc
     2401 ctcctccata cccaggcgac tcatgacccc agagatgcgc tgggacccga agattcggag
     2461 caggtcgtct tccagagaga gatagaatcg ggaagtcccc gggtctccct ggcgtccgga
     2521 acggccccgc agctggttgt cgatccgccg ggactcgtgc ctttcggtgc ccaggacgtg
     2581 aaggccgccg gcttcgatga ccttctggta ctcctgggag gtgaggcctc tcatcccttc
     2641 caggatcttc tgccagcccg cgggatcggc ggccgggtcg attttgcgct tccgggcttc
     2701 ttcccgggcc aggccttcgg ggtttccgcc cagcatgatg tccgttcccc ggccagccat
     2761 gttggtggag atggtgaccg acttgtatct ccccgcctgg gcgatgatgt tggcctccat
     2821 ctcgtggtac ttggcgttga ggacctggtg ggggacctta tccgccctga gcatctggga
     2881 gagcttctcg gaattctcga tggtgatggt tcccatgagc accggctgcc cccggcggta
     2941 gcagtccttg atttcttcca ccacggcttg gtacttttcc cgctgggtcc ggtagatgac
     3001 gtctgagaag tcctcccgga tcatcggcat attcgtggga atgaccacca cgtccagttt
     3061 gtagatcttc tggaactcgg cggcttcggt gtcggccgtg ccggtcatcc cggccagctt
     3121 cttgaacata cggaagtagt tctggaaggt gatggtggcc aaggtctgat tctcttccgc
     3181 caccttcacc ccttccttgg actcgatggc ctgatggagg ccgtcgctcc agcggcggcc
     3241 gggcatcatc cgccccgtga attcatcgac gatgatcacc tgcccgtctt tgatgacgta
     3301 gttcacgtct ttctcgaaga gccagtaagc cttcaccagc tggtggacag tgtgaatccg
     3361 ctcggaggac tggcggtacc gggtttccaa ggcttccttg gcctggacct tttctgaagg
     3421 cgaaagggtc gggttggaat cgatctccac atacccttcg tccagggagg ggaccacgaa
     3481 ggcgtcgggc tgccctttgg aaaggagctc catccccttt tcagtcatct ctacggcatt
     3541 ggatcgctcg tcgatagagc agtagagttt tcccagaaac tgagagaggg ttttttgccc
     3601 ggaaaggatg gattccagcc ggtcgatttt ggttttaaga gcgggctccc gggcgatgag
     3661 gtccaaaaac ccggggtgct tggggtcccc ccgcttcacc aataggagtt tttcgatggc
     3721 ttcttcgctc tccggctctt ctttgaggac cttcagcgcc tcttggagga ccccatccat
     3781 caaccggctc tgactctggc gcagccgggt gaccaaaggt ttcagctcct cgtaataccc
     3841 atcttccagc accctttcca ccgggccgga gatgatgagc ggagtgcggg cttcatcgat
     3901 gaggatggag tccacctcgt ccacgatggc gtagttcaac tcccgctgga ccatgtcctc
     3961 caggcggaac ttcatgttgt cccgcaggta gtcgaaaccg aattcgttgt tggtaccgta
     4021 ggtgatgtcg cagcggtagg cctgctggcg ctgggcgtcg tccagctcgt ggacgatgac
     4081 ccccacggac atgcccagga agtggtagat ggaacccatc catgccgcgt cccgcttggc
     4141 gaggtaatcg ttcacggtaa ccacatggac gccctgaccg gtcaaggcgt tgaggtatac
     4201 agggagggtc gccgccaggg ttttcccctc gccggtcttc atctccgcga tcttcccctg
     4261 gtggagcacc atccctccga tgagttgcac gtcgaaatga cgctgtccca aggtccgccg
     4321 ggaggcttcc cggacaacgg caaaggcctc gggcaggagg gtttcgaggg cttcaccctt
     4381 ggcgagtcgc tcacggaact ccggggtctt ggctttaagc tgggcgtcgg tcagcttcag
     4441 gacctccggc tccagctcgt ttatggcctg aaccaaggga tacagccggt tgatctcccg
     4501 ttcgtttttg ctgccgaata ctttccggag ggcttcgaag agcatggctt tttggttaca
     4561 gagattagag acaaatggaa atgaacaacc acaagggcaa cggatgggtg agtctcctcc
     4621 ccatcgtcgg tccaactcgg atctcgggtt ttaacctttt cccctcttgg ctctcttggg
     4681 cctttttggc ttgaagtaaa gggaatagca aagaaaaacc ccgataaagc attttccctt
     4741 atcggggtcc aaaattcaaa cccattataa aaatagcata agcatgggct gaagaaaagc
     4801 gaaatttatg aggtctgggt gaggtaggag aagggattgg cgggtcggcc gtggatcctc
     4861 acttcgtaat gcaagtgggg gcccgtggtg ttccccgtgg tcccgacggt gccgagcaat
     4921 tggcctgtct tgactcgatc cccgggtttt acccgaatct ccttgaggtg gccgtacatg
     4981 gtggtaaaac catggccgtg gtccacacag atcaggagtc catattcggc ctcccggcca
     5041 gccgataata caaccccatc cgccggggcc acaatcgggg ttccggtggg agccgaaata
     5101 tcaattccca tgtgaggccg ggttcctccg gaggaggggc tgaagcgaac ttcaccaaag
     5161 gaggaagtaa ttctcccgta gaccggccat agggaaggga tggaaagaag gactgacttc
     5221 tgggcctgaa ggaactcctt caagtctttg agggtacgct cccgttggaa ggcttccttg
     5281 gaaaggtcca tcagctccat atacagccgg ctgaccagct tgggtctttc cctttccagg
     5341 atcgaaacct catcctgacg caaaaggagc gccttctggt ggggcggcaa atcttgttta
     5401 ttttcggaag ttttctgggg aacgctgggg agtggggtaa gcttttttac ctttttttcg
     5461 tttttcaatt cctggacttc tttcaggtct ttcttgacct gctcttccac cagcttgagg
     5521 cgggtcagct gctcttccag gaggctaatt ttttccagga aggattgaat ttctgcctgc
     5581 cgagaattca attcgttgcg gagttctttt aaatcgaaga tgttcttctg ataggtgata
     5641 tagtcgtaga aaaggtatgc ggatacaacc aagaggagag ttaagaccgc cgccccgatt
     5701 tttagggtta aaggtgagaa aataaatctt ttaaccctgg aggcctgctg ggagaacact
     5761 aaaacggtgt actttttttt agccaaacct tagctccaag agatcgacgt ccttgaaccc
     5821 gtaggtctaa agggggccag ggtttcagac tcaacctaaa aaccagccac cgttttcctt
     5881 ttgccccact gagggtctcg tttaacatag tcaaaggacc cttgtcaagc ccttttttta
     5941 caaaaaaatt ataactatac ggatttattt atttttgtat tttcgactga cccatgttcc
     6001 catttcgtat ccctggctaa tgttccatac agagtgtaat taacctatgc ttatacctcc
     6061 aggagcacag caatctcatc acattctggg aatttaacgc attttatgca gtcggcccag
     6121 attttatggg gaagtgcggc cttgtccaca accttgaaac catggttctt gaagaaatct
     6181 ggccgatagg tgagggcgaa aatgcggtaa aggccaaggc taactgcttc tgaaaggcag
     6241 gcttcaatca gcttgcttcc gattccctgg tgctggtatt cctcctgcac gaccagtgag
     6301 cgaacctcgg ccaggtcctc ccaacagatg tgcatggcgc aggtaccaat aatcccttcc
     6361 agcccatcct ctacatatac ataatgatcc ctcaggttat cataaagttc gctcaaagac
     6421 cggggcagca tctctccctt tttagccgaa cgttcgatga gcttctgaat atcccggaca
     6481 tcgctgattt tggctttgcg aatgggcatt tcctgaaacc tctcgatcct ggatacaaga
     6541 tactggggat actggacacc agatgctgga taaggcattt ttattagcag aaagcttttt
     6601 tcgacatgcc tcctgaattc tcgttccagc atccaggatc aagcatccag catcatttta
     6661 ttttttagcg ttttttagca tctcccgggc atgttccagg gtcttatcgg taattcggac
     6721 ccctcccagc atacgagcga tctcttccaa acgctcctcg tcgctgagtt ccttgacttc
     6781 cacaaaggtc ctgcccccgg attccttctt ggacacgcgg tagtgagagt cggcaaagca
     6841 ggcgatctgg gggaggtgag ttatgcagaa cacctggtgt tggcgggaga tctctttcaa
     6901 tttccgaccc acgatttcag cgatccctcc accgatgccg gcatccactt cgtcaaaaat
     6961 gagggtcttg accgaagact cttccgcgaa gatgcgcttc atggccaaca tgattcggga
     7021 aagctcccct ccggaggcga tcttcgccaa gggcctcagt tcctctcccg ggttgggcga
     7081 gaggaggaat tccacctgat ccatcccctt ttcgttcaac ggagggcgcg gacccccatc
     7141 ggcctcattc ccttcgttcg ggtggacttc tattcggaaa cggaccttct tcattcccag
     7201 ggtggaaagc tctttttcca gcctggctcc gaggtctcgg gcgatgccct ttctcttgcc
     7261 agaaagctcc cgggctagct ccgccgcccg ggcgagctct tgagcggcag tcctttccca
     7321 ttgggagatg ctttcctcca agctccccaa gctttttctt tccccgtcga ttttttcctt
     7381 gtaggccgag acgtcggcca gggagggccc atattttttc tttagtcgat tgagttcatc
     7441 caatcgggac tctatggcct ccaggcgttt ggggtcgaaa tagacctttt cccggtaggc
     7501 cctgagggcg gaggcgacgt cttcggactg aaagaggatc gattcgacgg aagtcaccag
     7561 cggacccagg gtggaatcga ccttggcccc ttccttcagc cgctgcaggg tggatttcag
     7621 gcgctccgcc acagaaccgc tctctccgta aagggcttcg gtcccgaaat gagcgatgga
     7681 atggagtttt tccgaatgga ccaggcgact tcgttccccc gccagttctt catcctctcc
     7741 cggcttcggg ttggcccggg tgatttcccc cgattggaag gcgagaaact cgatccgttc
     7801 caatcgctgt ttctgccggg aggtcaaatc gctgagttcg gaagccgcct tcgcccaacg
     7861 ggcatagact tcttggaacc gggctctctg gtcgaggagc cccccggtgc tgtccaaaat
     7921 atcgatatgc cgcagggggt ccaggaagtg ttgatgctca tgctggccgt agatgtgaat
     7981 caactcttcc cccagccctt ccagcatgtg cagggtgatg gggtttccgt ttagaaaagc
     8041 ccgggatttt ccggaatggt ggatgagccg ccggacctgg agaccctccg actccttgag
     8101 gcccagattc ttcaccaggg tgaccttggg tccttcctct agatcaaaaa aagcctccac
     8161 cgaagcctcc tcccggcccg tccggatcat ctcggaagag gcgcgggtcc ccagaaggag
     8221 gttaagggca tcgaccagga tggatttccc ggcccctgtc tctccggtga gggtgttgag
     8281 gcccggcccg aaacgggcat gaatctgttc gatgatggcg aagttcctga tggtcaggtc
     8341 cgagagcatg atgcctgctt ttccgatctt gtcaccgctc tccccacttc aacttggtgc
     8401 gcaggatctc aaaatagtct cggtagggag atttaacgaa aaggacatgg ttctgtgcct
     8461 tggtgatgca gatgcgatcg tacgggagga ggttacaccc gatctggcca tccagggtga
     8521 gccggacgtc gctttcctcg gtctccaggg tgaattccac cgtcgcccgg tccgggatga
     8581 ggagaggccg gttggtcaac gtatgagggc agatgggggt gatgaggact gaatccaggg
     8641 aaggatagac gataggacct ccggcggcaa gggaataggc agtcgaaccg gtgggagtgc
     8701 agatgatgac cccgtcccct cggtatgtgg tgacataggc cccgtccacg ctgttccgga
     8761 ggtgaatgat ccgggccaag acggtcttgc tgatgaccgc gtcattcaag atggtgtact
     8821 cccccacctt ctccccctgg cggatgacgg ccacgttgag cctcattcgt ctttcggttt
     8881 ccacctttcc ttccagggtc ctttccagga ctgggtagag ttcctccagg gtgatctcgg
     8941 tgaggaaccc gaggccgccc agattaacgc ccaggatggg aaccttttcc ccccccaggt
     9001 gacgggcggc actcaacagg gttccatccc cccccaggac aacgagcaag tccacctcca
     9061 ggccaatctt ctcccggggg caggcaagtg cgggattaag ggaggaagca acggatgggt
     9121 ccaaagccac ccggatgttt ttccgcccca gccatccggt gagctcccgg gcgacggaaa
     9181 tcgcctccgg cttgtcgggt ttgaccacga ttccaatacg gttcagcaaa gcaggctcct
     9241 caagatattt ccagggcgaa ggaatttggg ttttgagtca cgaggattgg gtcggagacc
     9301 tcttgaactc cccttcgtgg ctctaatctc acaccccgga ttcgaagcga tctgttccag
     9361 gagtgctgat tttgtttttg gggggatttt attacaaaaa aagggggctg tccataaaga
     9421 attctacctt gcccacctta acttttttga atcccttttc tgctcgaaag caagcttgtg
     9481 gcccggaagc aagggtgttg cgcgcaaaaa gtatcggcgg gaaaagaatt ccgcccggag
     9541 atcctaccgt gcatccgcag aatggggcgg gcccatcggg cgcccggcgg ctcgactgag
     9601 ctcgccgaag tccgggcgcc cctaatcgga atcagctagg tcttgggcaa agaacggctt
     9661 gctcgcccgg cccaagaaac gaggtgggcc ttatggtttt tgcgcgcaac acccttgttt
     9721 ccgggccagg caaggcttga gatttaatcg cccacctact tcgggaaaag tgaaggtagg
     9781 aagaattccg ctgggtgttt ttctatttta tccaaggtaa gaagaaccag gaatggggat
     9841 tgaatggggg gaacatctat ggtagtcttg ggcttaattt gggaggccgt gaaccgcatg
     9901 gagctatttg aggcttcaag gcccaagctg gaagagagac gggaccttgc tcctctggcc
     9961 gaccgggtcc gaccggaacg gctggaagac tttgtaggcc aggagcacct gctcggccca
    10021 aagagatttc tccgctcctt gatcgaaaag gatcagctcc aatcgctcat cttctggggg
    10081 cccccgggga cgggaaaaac caccctggca tccatcatcg cccgttccac ccgttcccac
    10141 ttcgtggctt tttccgccgt actctccgga gtgaaggaga tccgccaggt cgtccaggaa
    10201 gcggaagaac ataaagcgcg cggccgaagg accattttct tcgtggatga aatccaccgc
    10261 tttaataaag cgcagcagga tgcctttctc ccacacgtag aacgggggac cattatcctg
    10321 atcggggcga cgacggagaa cccatccttc gaggtaaact ccgcgctcct ttcgcgctgc
    10381 aaagtcctga ccctgcgcag cctgacagag gaggagatcg gacgaatcct gacccgggtc
    10441 ctcagcgatg aggacaaggg cctgggaaaa ttgagggtgg aattagcccc ggaggcggta
    10501 aggttcttga ccgagatggc tcacggagac gctcgaattg ccctgaacgc cctggaggcg
    10561 gcggcgttga ccgccacgcc cgatgcccgg ggtctccgag cccttaccct ccccatggtg
    10621 gaggaggcca tgcagcgcaa ggcgctcctc tatgacaagg agggagagga gcactataat
    10681 gttatctccg cctttcataa gagccttcgg ggaagcgacc cggatgcggc cctctactgg
    10741 ctggcgcgga tgatcgaagc cggggaagac cctttatacg tggcccgccg gatggttcgc
    10801 tttgccagcg aggatgtcgg tatggccgac ccccaggctc tgggcgtggc cctctggtcc
    10861 atggaggcct ttcatttcgt ggggctcccg gagggagaac tggccctggc agaggccgcg
    10921 gtgtatttag ccaccgcccc caagagcaat gccatttaca cggcttacga aagcgcaaag
    10981 ggggatgtgg ccaagaaagg agcccttccg gttcctttcc ccattcgaaa cgcccccact
    11041 gccctgatga aggaattggg gtatgggcgg ggataccgat atgcccatga ctacccggag
    11101 gggttggtgg agcaggaata ttttcccaag gagctgggaa accggattta ctatcgtccc
    11161 atggaaaggg gcttcgaaaa aatcatcgga cagcgcctgg aagcgtggcg taaaaagatg
    11221 gggcgaaagc tctgaggggg tttggtaagg accctctcag gcttccattc aaagagagga
    11281 gtagaaacat gggtagtgtg gtgaagaaga gaaggaaaaa gatctccaaa cacaaacacc
    11341 gcaaacttct aaagagaacc cgggtccaaa ggagaaaaag aaagtaacca gcccttttcg
    11401 agaaggaaaa aaaggaaaaa ggttactccg gggtgggggc ggggggcggc aggatgtcca
    11461 cccgctcctt caattctttc ccaactttga aaaagggcag ccttttgggg gagacatcca
    11521 ctttggtccc cgtccgggga tttcttccta gataggactg gtagttccgg acggtgaagt
    11581 tgccaaatcc gcggatttcg attctttccc ctttgaccag agcgtcgatc attgaggaaa
    11641 agatcgtgtt tacgatggtc ttagcgtcct gctgggccaa gttgttcgac ttttccatga
    11701 gtttctggat gagctggaac ttggtcattg cttccttctc cttatgaaag gtgggttctt
    11761 ggggtttgtt agaaagtata taccatgaag ccttttggaa tgtaaaccta aaaatttaaa
    11821 aggaaatttt gccgaaacag ctcccctggc aaagggagct tcatccacct ttcatccgaa
    11881 tcatacaaaa ggggattcgt acccttcttt tctgcaaggc tcggatatct gttcttggtt
    11941 cttgcgctcc agttcgcgga tcgattctct aagggagcgg gcccgcttgt ccaggtcctc
    12001 cagttttcct tctttggcgc gaatttctgc gtctcctttg cagggctgga ggcggaggcc
    12061 tgattgctct ttttttatct cggccaattc cctctgaaag tcccgaatct ggcgttcaat
    12121 ctgttttacc atttcttccc tcttaaaggg cgatagggat tttataaagc ccgtggggga
    12181 gattccttga tttccacgaa tggtagcgat tttataaaat ttttgagaat caatcaagcg
    12241 caaaatgaac cttttttata ttatccggga acggccgaga atggaaggct tctcctttca
    12301 attagggcaa agcggatggc caggggtagg ttcccttgat tttcccgggt catcggaagt
    12361 agaataagtc cccaaaacga accgatggat tttggctgag tgaccatgaa ggggcgcgtg
    12421 aatcagtcca ttcccaaaat gcttttttgg gcgtcgatct ttcttctctt ggcgggttgc
    12481 gctacccccc tgagcgacgt ttcccaagac tttaagtccc aacgccccac gcggattgcc
    12541 gttcttccgg ccaagaacga aacggccgat atggacgggc ccattgtttt tcgccttctg
    12601 gcaggggcgg aattagcgga caagggttat gccctggtag acttcgcccg gatcgatgaa
    12661 gcccttcggg aaaaagggat cgaggaggcc gggcagattg aatccttaac ctcccaggag
    12721 atcggggaat ccgtaggggc tgacggtctt ctttacgtta cggttttgac ctatggccgc
    12781 caagtcggag ttcacctgaa gatggaggga agtttcatcc ttgtggacgc gaagaccggt
    12841 cagaagctct ggttttcgga gctctccgta tccgacgacg tcgtcctgga agggggaacg
    12901 gctgttttga taggagaact gtttgggggg caagataaaa gaaagagccg agagaaggcg
    12961 atggaaacct atctggccat gcgccaagcc aggattgctc aagctgtcaa ccggttccgc
    13021 gcccacccca tcaagcgaga agtgtttcgg gtgattacta cggacatgga taaaatctac
    13081 ctcctggaaa tattcttccg cagaaatttt cggagtctgc ctcgcccttg aggcgcctaa
    13141 gcagaaaagg taatttagtt cggagaaata aggttcggag gggaggataa taagtagagg
    13201 tgggtttcaa acctgcccct actccgacct ttttctataa aattcccttg aaaaaacccc
    13261 cgctcttggc tatattcgga ttgttccgat ggcggccttt acccgtgaat gagcggagac
    13321 cctgagttcc cagaaccttt ttctgttgaa aggcatccta attgataaac ttatccgatc
    13381 aatcccttat tgaaaaggtc cggggtgggg attaccaggc ttttgaattt ttagtcactc
    13441 gctatgaggc gaaggtttat cggctggcaa tccgcatgct gcgcaacccg caagacgctg
    13501 aagacgctct ccaggagact tttttgcagg tctttcgggg attgtcgggg tttgaaggca
    13561 ggtctcagtt ttctacctgg ttgttccgcc tggcaaccaa tgtttgcctg atgaggattc
    13621 gccaccggga aactgagcct tccaaactgt tacccctgga ggactatctt cctaagctgg
    13681 aggaagggga gggcccgccg atggtggatt gggcagaccg gccggaggac gccctcttga
    13741 gtaaggaatc ccgggaacag atgatgggag ccttggataa gcttcccccc gaatacagag
    13801 ccgttttcat ccttcgcgac atcgaaggtt tttccaatgc cgagaccggg gagtccctgg
    13861 gaatttccac agccgccgtg aagtctcggc ttcaccgggc tcggctggcc ctccggggga
    13921 tgctatctga atatttccaa cggaagcagg atttcggagg gctcgaaacc aggttggagg
    13981 attgaattat ggagaaagat gaaaaaacta cccaggtctt ttcctgcaaa ggaatggaat
    14041 gcaaggaaat gctggaaaaa ctctccgaat acatcgatgg ggaattggac ccgaaaatct
    14101 gccaggacct ggaaaagcac atggccgact gcaatccttg cctgatattc gtgaataccc
    14161 taaaaaagac cattagcctt ttcaaatatg ccagttccga acccctaccc aaggaagtcc
    14221 atatccgcct gcatgactac ttgaagaaaa attgccaggg ctgctgaaat cccgccttgg
    14281 cctaaatcct cccaacatcc ccccggatcg tcccgataga ttaatacctg aaggccagaa
    14341 tcgaaagaca gcgtagccct gttttttccc ttgacgaccg gaacccgttc ggtttataaa
    14401 aacatagcgc cgattggcaa gggagggttg aaaaggtgaa aatccagaag atgaaggcca
    14461 ggtcgaaagt ctggatcgaa gttggagggg aggtcgtggc cggggacgga aaggtcgacc
    14521 tgctggaaag gattgccgag acgggttcca tccagaaagc cgcgcaggaa atcgggatgt
    14581 cctaccgcca cgcctggggg ttcctccaga aaatggagaa gcggggaggg atcaaactcg
    14641 tggagaccca ggtgggcgga agggaagggg gaggagcgaa gctgacccct ccgggaaagg
    14701 aatttttgag gcggtattcg gccttccgcg aaggcctgga caaatatatc gaggataaat
    14761 tcaagagagc atttaaatga accatcagcg gtcagctatc agcttcagca aataaacatc
    14821 ggatggcttt tagctgatcg ctgaatgctg attgctgaga gctgcttttc tatgcgtttt
    14881 ctgggcaacg aatataaccg taatgaactg gccggagcct tcggagacct gggcaccttc
    14941 atcccctttg tggccgccta catcaccata aataaaatgg accccttggg catcctggtg
    15001 gccttcggcg tgttcaaaat cttcgtgggc cagtatttta aaacccccat gccggtccag
    15061 cccatgaagg ccatcggggg tatggccatc gcccatccgg agagcatcac ccaagggatg
    15121 atctggggct cgggattatt caccgcggtc ttctggctga tcttggggct ctcaggggcg
    15181 gtctcatggc tatacaagat caccgccaaa cccattaccc gcggcatcat gctggggttg
    15241 ggcctttcct ttgtcttgca aggggttggg atgatgaaag aacagccgtg ggtagccctg
    15301 attgcagcgg ccatgacttt ttttcttctt tcccaggaga aaatcccggc catgctcctc
    15361 cttctggctt tcggcatggg ggtggccttc atggccaatc cctcgctatg gcaggaccta
    15421 agccagattt cggtcagttt ccgatttccc gaattatctc tgggcaggat gagctggtcc
    15481 gacctggtcg cgggaactct gatcctggga ttgccccaag ccccgcttac cctggggaac
    15541 gccattatcg ggacggccga ggaaaataac gaactcttcc cggatcgtcc ggcaaaggtc
    15601 aaaaccatcg cggttgacca tggcatcatg aacgtcttca gcaccgccat cggggggatt
    15661 cccctctgcc atggcgccgg gggaatggcc ggccacgtcc gcttcggcgc ccggacgggt
    15721 ggagccttga tcattctggg aatcatcgtc ctgcttctgg gccttttctt cagcgattcc
    15781 gttgccacga tcttcaagat gtttcctccc tccatcctgg gggtcattct cttcttcacc
    15841 ggcctggaac tggcttccat cacccgggat atcggaaacc agcgctcgga cgtttatatc
    15901 atgctggtcg ttgccggcct ggctatgtgg aacatggggg cggcttatct cgccggcctt
    15961 gtcctttatt atggggtgga aaagaaggtc ctgaagttct aggtcttctt taccccttgg
    16021 ccgtgaaatg tgatagctcg taaatgccca atgatttttc cagcccggct acttcttcta
    16081 ccaccttctc aaatccatgc tccccaaaga gcttatcttc ttgttcctca aatttctccc
    16141 ccaacgaatc atattccttt gccgaaacga ccgactgaaa ggcagggaag agaaccgtat
    16201 cttccctcga tttatggggg cgatacatcc gggcgaagag gcccaggttt tttaacaatc
    16261 ctctcttctc atccggatta agagttgata gggaaccaag attttttatt ttttccgtta
    16321 gccggcgccc tgcttgatgc tgttccagca agacattgac gagatcgacg agtttcccca
    16381 cctttacaaa tcgagggaat acgtgatcct cctcgagttt ctcatggtag tcttcaacaa
    16441 atcggcgaat caggaccgcc gccccggtaa gcacttcagg agggtatttt atgtccttct
    16501 ccaggcgcct ggaaatatca tcgtagatga gcaaaattcg tgagagaacg ccgtgttcgc
    16561 gcatcaggtc ctcagctgga gaaatttctt cccctttttt cccctcgtac ttcttgtcct
    16621 tgcttttgga agctgccagg gttgaagcac catctgtttg taggatggcg gtcgaagcca
    16681 ttaaaattcc gccctttttg ataaactctc tgcgagtgga aaggatttcg acaaacctct
    16741 tcataagcca cctcctgctc gaatccggga atttattcat cgcggggaag attatcttat
    16801 ttatctgcct gaaaagagag tgaaaaaatg gggtgaaata tgtattttaa aaagggaaat
    16861 cccgtatttt ggggttattg ggccgcaaat gaaaagactt gggtcaagat aaatctgcag
    16921 ccgcaaaaag gtggaggatt tgcagactcc tccaaaaaac gaggtgatgg atttcatcgc
    16981 tgatcgggca ttagaataag gaggtgaatc aatatgaaat gcgatcgttg cgacaagacc
    17041 ctcgaatccg gaaatgaacg aaatcatctg ggccggaacc tttgtgaaga ctgctacatg
    17101 gacgccctgt caccctcgcg agcctgcgac ccctgggcgg ttcacagcgc gaagtctttt
    17161 gaaagacagt cgggtggcac cggggccctg accccggtcc aatccgagat tctgtgcctc
    17221 ctgagagaaa ccggcggcat ggaacctccc gttttgcttc ggaaacttgg cggcagactg
    17281 acgctgaaag aactggaacg ggaatttgcc accctgagac acatggaaaa ggccagagga
    17341 gagaaacgag gggatcgaat tgtctggcgg ttatggtagc gctttaaggg ccggacttga
    17401 tattttggat tcttcttctt gcgttggtcc atgcctctta aatcccctac ccttcacatc
    17461 ttgaagggac ccggcactct ctcatcctgc cttcaacctg cggtcttgta tcccatgttt
    17521 ccccttgaca gcctcatcct tattacccat actcaacgta agggagttca ttattctagg
    17581 aaaggggggt gttcatatga aggaggagga aaagatcgcc cagcttaaga agatggtgaa
    17641 tcagctgatt aaagttgagg aggaagtgat tgctctgacc gacaaaacgg ccggtcaagc
    17701 cgcgaattca accgtaaagt atttgcttcg ggccatccag cacgattccc gaaaacatgc
    17761 cgatttgggc cgggccgccc tggaaatcct cgagtacaaa caagtagcgg gagcagaaga
    17821 gagaaaagaa gtctgggacc tgttaaaaaa acatgcggaa ttggaaagaa aacaccagga
    17881 ttttctttcc gagatgaaga aacttaccga tacccccgcc ctgaaataca tttttgaaca
    17941 ggtctggaat gacgagaaaa tccaccatgc catgcttgaa acgatgatga ccaaacgatt
    18001 cgagaaagac ccggcgaagt attggggggc cctgctaggc tcgtaaaagg agtggcataa
    18061 accttgctcg ggaagagcgc ttaaagaaat ggaaggccga ggaacaatct cggccttctt
    18121 ttttaaaatc ttcgaagcga ttcatgatgt ggggaaccta caaaaggaat ccaaaaagta
    18181 aaagggaaag gattgtccct gtcaaaagga tgtaggaaag atcgattttt tttagaagag
    18241 caatgaaggc cccaatggtg aaaaggatac tgggaatatc cacaaaggtg gcccggccga
    18301 aattatagag caccaggccg agcatgccga cgaaggatgc caggatcccc acttcaaaaa
    18361 ttcggactgc ggctattccc cgcactttgt cataatgagg aatcaacagc gttacgatat
    18421 agaaggccgg agaaaaaatg gccagggtag tcatggccgc cccccagaag ccggaaagtt
    18481 tgtatcccag aaaggtggcc gtgatcatga tcgggcccgg cgtcacttgg cccatggcga
    18541 tcccgt
//
LOCUS       DASPRR010000520         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_127494, whole genome shotgun sequence.
ACCESSION   DASPRR010000520 DASPRR010000000
VERSION     DASPRR010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127494"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VEL68_19385"
     CDS             complement(<1..188)
                     /locus_tag="VEL68_19385"
                     /inference="COORDINATES: protein motif:HMM:NF013577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HXZ38198.1"
                     /translation="MILLHREIKGRGNQLSQAQRKLLEYVLAHEEEAVFLKIGDLARQ
                     GKVSLATVSRLSRALGFKG"
     gene            437..1333
                     /locus_tag="VEL68_19390"
     CDS             437..1333
                     /locus_tag="VEL68_19390"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ38199.1"
                     /translation="MKPGIGFIGLGTMGKGMAFNLIKAGFPLKFFARKKAVIEETIRQ
                     GGQMIPSPRDLALQVTWMFLCLPGTETVEEVLFGREGIAEVMKSGQVLVDCSTIHPLA
                     ARRIHRTLQEREIPFLDAPISGMEARAKAGTLTIMVGGEKLAFEEVRPALEAMGNTIL
                     YLGASGNGQIMKMINNVLFDIHCAALAEILPMAVKIGLDPEQVVTVARKGTGQSFALD
                     NFGPLILEGNFGPGYPLADAYKDMVHAVEISSAHRIPLPVTAATMATYQMALDEGLGR
                     ENKGAMIKVWEKVLAVQVRRKA"
     gene            complement(1448..>2437)
                     /locus_tag="VEL68_19395"
     CDS             complement(1448..>2437)
                     /locus_tag="VEL68_19395"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXZ38200.1"
                     /translation="KDLDIPKPGPRDVLIRVKVGAICGSDVHIFEWDPWAQSVVPKLP
                     QILGHEFAGEVVEVGREIHTLKPGDHVAGETHIPCDNCYFCLTGQRHICQYMKVYGVH
                     SHGIFSEYTSLPEPCAVKIPDEISWSSAAMMEPCGVAVHGLSKGNVSGKTLAIFGCGP
                     IGLFAIQAALSYGAFRVYAADVSANRLKMALSFGATKVFQAGKDPLEKVILEETRGIG
                     VDMVLEISGAQQAYDQGLKILRKAGTFVIIGVAAKPIQLEIPTNIMYKEAKLFGITGR
                     EMYETWHQVINLVTSSKINLEKVATHKFPPEKFEEGFKIAKEAQHGKVEFMFG"
BASE COUNT          557 a          664 c          695 g          521 t
ORIGIN      
        1 cccttgaatc ccaatgctct ggatagccgg gaaacggtgg ctaggctcac ctttccctgt
       61 cgcgctaaat ctccgatttt gaggaagacc gcctcttcct catgggccaa cacatactcc
      121 aggagtttgc gctgggcctg gctcagttgg tttcctcgcc cttttatttc ccgatgcagg
      181 agaatcatag ctgaaagtat tttttctcaa agagtggaaa gtgaaaattt tatatcctga
      241 gggagggaaa atgtcaacaa aaatcgaaga gtgtgttcat gattcgattc cttcgcggcg
      301 gcggctgggc taaaggtgac cggttcgccc cccagatagg cagtctgccg ctgcggttgc
      361 aaattcggag ggggcatatt atgctcagag gaaaaaaagg aaggtctgag gatgcggctt
      421 acctgaagga ggcagaatga aaccggggat agggttcatc gggctgggaa cgatgggaaa
      481 ggggatggcc tttaacctga tcaaggccgg gttccctttg aaattcttcg cgcggaagaa
      541 agcggtcata gaagaaacca tccgccaggg agggcagatg attccttccc cccgagacct
      601 ggcattgcag gtgacctgga tgttcctgtg ccttccgggc accgagacgg tggaagaggt
      661 gctcttcggc cgggagggga tcgccgaggt gatgaaaagc gggcaggtcc ttgtcgactg
      721 cagtacgatc caccccctgg cggctcgcag gattcaccgg accttacagg agcgggaaat
      781 ccctttcctg gatgcgccca tttccggcat ggaggctcgg gccaaagcgg ggactctaac
      841 catcatggtg ggaggggaga aattggcctt cgaggaggtg cggccggctc tggaggccat
      901 gggaaacacg attctctacc tgggggcttc cgggaacggg cagatcatga agatgatcaa
      961 caatgtcctc ttcgacatcc actgcgcggc cctcgccgaa atcctcccca tggcggtgaa
     1021 aataggccta gacccggagc aggtggtcac cgtggcccgg aaagggaccg gccagtcttt
     1081 tgccctggac aacttcggtc cattaatcct agagggaaat ttcggccccg gctacccgct
     1141 ggccgacgcc tacaaagaca tggtccatgc tgtggagatc agcagcgccc accgcattcc
     1201 cctcccggtg accgcagcca ccatggccac ctaccagatg gccctagacg agggcctggg
     1261 aagggagaac aagggagcca tgatcaaggt gtgggaaaaa gtcctggcgg tgcaggtaag
     1321 aaggaaggcg tgaaaaagtc gaatagtcgg gtacgttcct aggtgaatag attcatcgct
     1381 attcaccagc aaccctgaac tggagattca gaaaaaaatt agctgatccg accctctgac
     1441 tattcgacta cccgaacata aactccactt tgccgtgctg ggcctctttg gcgatcttga
     1501 agccctcttc gaatttctcg ggggggaact tatgagtggc caccttctcc aggttgattt
     1561 tgctggaagt aactaagttt atcacctgat gccaggtttc gtacatctcc ctcccggtga
     1621 tcccgaagag cttggcctcc ttgtacatga tattggtggg aatttccaat tggatcggct
     1681 tggcggccac gccgatgatc acaaatgtcc cggccttccg cagaatcttt aaaccctgat
     1741 cgtaggcctg ttgggccccg gaaatttcca ataccatgtc cacgccgatc ccccgggtct
     1801 cttccaggat gactttttcc aaggggtcct tccccgcctg aaagaccttg gtcgctccga
     1861 aggaaagcgc cattttcaga cggtttgcac tcacgtccgc ggcatatacg cggaaagccc
     1921 cgtaggagag ggccgcctgg atggcaaaca gcccaatagg cccacagcca aagatggcca
     1981 aggtttttcc ggagacattt cccttggaaa gcccatggac cgccaccccg cagggttcca
     2041 tcatggcggc agaggaccag gaaatttcat cgggaatctt cactgcgcag ggttcgggga
     2101 gagaagtgta ctcggaaaat atgccgtggc tatgaacccc gtagactttc atgtactggc
     2161 agatatggcg ctggccggtc aggcagaagt agcagttgtc gcaggggata tgagtttccc
     2221 cggccacgtg atccccgggt ttcaaggtgt gtatctctct cccgacttcc accacctctc
     2281 cagcgaattc atgccccaga atctgcggaa gtttggggac cactgattgg gcccacggat
     2341 cccactcaaa aatgtggacg tcggagccgc agatcgctcc caccttcacc cggattaaaa
     2401 cgtcccgggg acccggctta ggaatatcca aatcctt
//
LOCUS       DASPRR010000521         5503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_935752, whole genome shotgun sequence.
ACCESSION   DASPRR010000521 DASPRR010000000
VERSION     DASPRR010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935752"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2150)
                     /locus_tag="VEL68_19400"
     CDS             complement(<1..2150)
                     /locus_tag="VEL68_19400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXZ38201.1"
                     /translation="MILLDMRTLVFSNVVTDILCLLVVFLLWRQSRNRFAGMGFWVFD
                     YTFQTAAMLLIILRGSIPDWASMVLSNTLVIGGALLGYMGLGRFVGKKVPQGHNYVLL
                     AAFASFHAYFTHVQPALAARNLNVSVALLIICFQCMWFLVYGVKPGMRRLTFGVAMVF
                     GGYCLVSLVRIAGFFTGTSGATDYFRSGAFESLILISYQMLFILLTYSLVLMVNKRLF
                     VDVQTEEEKFSKAFRSSPYAITLTRLSDGQIVDVNDGFLKISGYSYPEAIGKTTTDLH
                     LWDRPEDRGAAVAELGRSGKVEEREYQFRKKLGETLIGLFSAHIIPVGNDSYVLSSIN
                     DITERKKAEQALRESEAKANALIKYAPTGIYEVDYRTAGFINVNDAMCQILGYTREEL
                     FAMGPAALLDDKGRMLFANRIRRQLAGEKVEEAVEYRVRKKGGKFIDAILNVSFIAGS
                     EPGRALVVAHDITARKRAEQELEQARNTLAEAQKIAHLGSFEYVAATRTTMWSEEEYR
                     IYGLDPAGPSPAYELMLAKCIHPDDAALLHETFTQAMQTRSIYELEHRIVRPDGSVRW
                     VYDLAHPYFDEKGNLLRYVGATLDITERKRAEERLRRQQGEIQTLLENTPAGLVLFEA
                     NPPYKVLAHNRYYQELFAEPFRSQGMVGLNVYEYAPAVEAEGVVAVFDEVVRTRQPKS
                     FLDFPYKSDPPNQTWFNWHMSPLILDGEVVALVS"
     gene            2625..3011
                     /locus_tag="VEL68_19405"
     CDS             2625..3011
                     /locus_tag="VEL68_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ38202.1"
                     /translation="MPKYSFNHIHLLTRDPKSTAQYYHKMFGAKIIESTQSDGKPRID
                     LELAGVSIFILRVPPEQETPASPPGPYLGLDHFGLRVNNLDEAVAELKAKGAEFAVEP
                     KTIRPGVRIAYIRGPENARIELVEHA"
     gene            3192..3611
                     /locus_tag="VEL68_19410"
     CDS             3192..3611
                     /locus_tag="VEL68_19410"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXZ38203.1"
                     /translation="MPRGDREKRVKKLLAEMEKERGYVSLAKNYVATVDPDFMEAYNN
                     LYNNGLRAGRALPVKYRELVAIAILSYRTREDAVYLHMKRALRHGATVQELLEAVETS
                     IIPGGGPTFDTGTQALMRILEEEKEKKKKGGPGKNKR"
     gene            complement(3672..4430)
                     /locus_tag="VEL68_19415"
     CDS             complement(3672..4430)
                     /locus_tag="VEL68_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXZ38204.1"
                     /translation="MDIRVGCCGFPKARKEYYKVFRVVEVQQTFYQPPALKTAEKWRE
                     EAPADFEFTLKAWQLITHESKSPTYRRLKLSCPGNQLDKCGSFKPTEEVHWAWEKTRE
                     IAQALRSHYIIFQCPASFHPSAENKRNLETFFRKVKRENFRFIWEPRGTWTPEEIRDL
                     CRELDLIHGVDPFKADPQWGQLRYFRLHGITGYRYRFSGEDLRELQGKCRGMTYCLFN
                     NVNMWTDALAFQNRIQETGFRSQDSGTRNKKPET"
     gene            complement(4549..>5503)
                     /locus_tag="VEL68_19420"
     CDS             complement(4549..>5503)
                     /locus_tag="VEL68_19420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXZ38205.1"
                     /translation="VFPAYAQEGSKENVKELLKAVVKVRAVVPDYARSAKTLGTEREG
                     SGVLIDTQGHILTIGYLILEAETLEIVAPDGKSISAQSIAYDHITGFGILKAEKPMDA
                     KPMQLGDSSEVTEGDPILVATYGGEEGAMGARIISRKEFAGYWEYLLDDAIFTAPPHA
                     NFGGAALIDHNGKLVGIGSLFTQISIPGLGMIPGNMFVPINYLKPILSDLINKGRSSA
                     PPRPWLGVNVEEVHGRVFVTRVTSESPAEKGGLKVGDIILTVNKKEVNGLADFYRKVW
                     ALGKPGVEVPLTLLQGIQVQDIKVKSGDRYQYYPLKPQKKI"
BASE COUNT         1253 a         1553 c         1471 g         1226 t
ORIGIN      
        1 atgctcacga gcgcaacgac ctctccgtcg aggatgagcg gcgacatgtg ccagttgaac
       61 caggtctggt tgggcggatc gctcttgtag gggaagtcga gaaagctctt tggctgccgg
      121 gtgcggacca cctcgtcgaa tacggccacg accccctctg cctcgaccgc gggcgcgtat
      181 tcatagacgt tgagtcctac cattccctgg ctacggaacg gctcggcgaa cagctcctgg
      241 tagtagcggt tgtgcgccag gactttgtag ggtggattgg cctcgaacaa gaccagcccg
      301 gccggggtgt tctcgagcag ggtctgaatc tccccctgct gtcgccgcag tcgctcctcg
      361 gcccgcttgc gctcggtaat gtccagcgtc gcgccgacat accgcagtag gttccccttc
      421 tcgtcgaagt acgggtgcgc gaggtcgtac acccaccgca cgctgccgtc gggcctcacg
      481 atccggtgct caagttcata gatcgaacgg gtctgcatcg cctgggtgaa ggtctcgtgg
      541 agcagggccg catcgtcggg atgaatgcac ttggcgagca tgagctcata cgccggcgac
      601 ggaccggccg ggtccaggcc gtagatgcga tactcctcct cggaccacat cgtcgtgcgg
      661 gttgcagcga cgtactcgaa gctgcccaga tgcgcgatct tctgggcttc cgccagcgtg
      721 ttgcgcgcct gctccaattc ctgctcagcc cgtttgcgcg cggtaatgtc gtgcgcaacc
      781 actagcgccc tgcctggttc gctgccggcg atgaacgaaa cgttcaggat cgcatcgata
      841 aacttgcccc ccttcttccg gacccggtat tccaccgctt cttccacctt ctcgccggcc
      901 aattgccgcc tgatccgatt ggcgaaaagc atacgtccct tatcgtcgag caatgccgcc
      961 ggacccatcg cgaacaactc ctcccgggta taacccagaa tctggcacat ggcgtcattg
     1021 acgttgatga agccagccgt ccggtaatct acttcataaa tgccggtcgg ggcgtatttg
     1081 atcagggcgt tggctttcgc ctccgactcc cggagggcct gctcggcttt cttacgttcg
     1141 gtgatgtcat tgatgctcga cagaacataa ctatcgtttc cgacggggat gatatgggcc
     1201 gaaaagagcc cgatgagcgt ctctcccaac tttttccgaa attgatattc ccgctcttcc
     1261 accttgccgc tcctgcccaa ttcggcgact gcggctccac ggtcttccgg ccgatcccag
     1321 agatgcagat cggtcgtggt ttttccgatc gcctccgggt acgagtaccc cgagatcttc
     1381 agaaaaccat cgttgacatc gacaatctgt ccgtcggaga gccgagtaag ggtgattgca
     1441 taaggagagg aacggaaggc cttggagaat ttctcttcct cggtttggac atcaacaaac
     1501 aggcgcttat tcaccatcag caccaggctg tatgtgagga gaatgaacag catttgataa
     1561 gagatcaaaa taagggattc aaaggccccg gatcggaaat aatcggttgc tccgcttgta
     1621 ccggtgaaaa atccggctat ccggaccaga ctgaccaggc agtacccccc gaaaaccata
     1681 gccacaccaa aggtcaagcg gcgcataccc ggcttgactc cgtaaaccag gaaccacatg
     1741 cattggaagc agattatcag caaagccact gacacattga gatttctcgc agccagggcg
     1801 ggctggacat gggtgaagta agcgtggaaa ctggcaaaag ccgccagcag aacataattg
     1861 tgcccttggg gaaccttctt tccgacaaag cgtcccaagc ccatatagcc gaggagggct
     1921 cccccgatca ccagggtatt cgagagaacc atggaagccc aatcaggtat gctgccccgc
     1981 aggatgatca agagcatcgc cgccgtttga aaggtatagt cgaagaccca aaagcccatc
     2041 cccgcaaacc tgttacggct ctgccgccac aataaaaaga cgaccagcag gcagagaata
     2101 tcggtgacca cattgctgaa gacgagtgtc cgcatgtcaa gcaagatcat ggatttcctc
     2161 cggacgttca gcggaaaaaa aacggttata cccgggcctt catcgggaac ttctcccccc
     2221 aattctgatc gtccccttgc ttccttcctc cctaatcccc cctacccctc ttaacttaag
     2281 aggaggagtt ttctcctccc cttaggttaa ggggaggcgg gggggttacc ctccaatttc
     2341 tcccttcccc ataaccccac cgaaaacagg gaaatgaccg gttggggata gctatctcta
     2401 gactctacca tttccacaac ttcgcaataa aactttccta ttttactacg aaaatcccct
     2461 gttcgtacca tcccctcgaa aagaattctg gccgtgcagc cggccaaaag ctcgtgagtt
     2521 gggagttgaa ggagtgagag tgccgatctt ttccctggac ggggatactc aataactggt
     2581 aaatttcgtc atggtatttg ccagcccaag aaaaaggagg tctcatgccg aaatactcgt
     2641 tcaaccacat ccatctactg acccgcgacc ccaagtccac ggcccagtac taccataaga
     2701 tgttcggggc caagatcatc gagtccaccc agtccgacgg gaagccgagg atcgacctgg
     2761 aactagcggg cgtctccatt tttatcctgc gggtcccccc ggagcaggag acgccggcca
     2821 gtccccccgg cccttacctg ggcctggacc acttcgggct ccgggtgaat aacctggacg
     2881 aagcggtcgc cgagctgaaa gccaaagggg cggaattcgc ggtggaacct aaaaccatcc
     2941 ggccgggagt gaggatcgcc tatatccgcg gcccggaaaa tgcgcggatt gaactcgtgg
     3001 agcatgcttg aaaaatcagg aatcaaagat gacgatttcg taaaaagtct cttgaaacgt
     3061 caccctggcg aaggcggggg gcatcccgcc aaggtcggga ttgaaataac tggactccgg
     3121 ttttcgccgg aataacgaat tctttggaat ttcgactttt tgcggaatca gcaaagatag
     3181 gaggatgaag aatgccccgg ggagatagag aaaagagggt gaagaagctt ctcgccgaga
     3241 tggagaagga aaggggttat gtatccctcg ccaagaacta cgtggccacc gtggaccctg
     3301 atttcatgga ggcttacaat aatctatata acaacgggct gcgggcagga agagcccttc
     3361 cggtcaagta ccgggagctt gtggccattg ccatcctgtc ctaccggact cgggaagacg
     3421 ccgtgtacct gcatatgaag agggccctgc ggcatggtgc gaccgtacaa gaactcttgg
     3481 aggccgtcga aaccagcatc attccaggcg gaggccccac tttcgatacc gggacccagg
     3541 cactcatgag gattctggaa gaagagaaag aaaagaaaaa gaaaggcggt cccgggaaaa
     3601 ataaaagata aaaaaatttt ctctcgcagg gttcgcagag aagaaaaaag ttaggtatca
     3661 ggaatcgggc ttcaggtttc aggctttttg ttcctggttc ctgaatcctg gctcctgaat
     3721 cctgtttctt gaatccggtt ctggaaggcc aaggcatccg tccacatatt gacgttgtta
     3781 aaaaggcagt aggtcattcc ccggcatttt ccctgcagct cccttaggtc ttcgccggaa
     3841 aaacggtagc ggtaccccgt gatcccgtgg aggcggaaat accggagctg gccccattgt
     3901 ggatcggcct tgaacgggtc aactccatgg atcaggtcca gctcccggca taagtcacga
     3961 atctcttcgg gtgtccaggt ccccctcggc tcccagatga atcgaaaatt ttcccttttc
     4021 accttgcgga aaaaggtctc caggtttctt ttattctccg ccgatgggtg gaagcttgca
     4081 gggcattgga agatgatgta atgggagcga agtgcctggg cgatttcccg cgttttctcc
     4141 caagcccagt gaacctcttc cgtgggcttg aaagaaccgc acttgtcaag ctgatttccg
     4201 gggcaggata gttttagtcg ccggtaggtg ggacttttcg attcatgggt gatcaactgc
     4261 caggccttca gagtgaactc aaagtccgct ggcgcctctt cccgccattt ttcagcagtt
     4321 ttcaaggcgg gtggctggta gaaagtctgc tggacctcta caaccctgaa gaccttataa
     4381 tattctttcc tggccttggg aaacccgcaa caaccaaccc gaatgtccat gaatgggttt
     4441 cgagctaaga ggttctgggt aatagggaat aggttacaag ttagaggttg gagtagggcc
     4501 tttgcctatc acctattacc tatctcctat gccccatgac cttatttttt atattttttt
     4561 ctgcggcttc agcggatagt attgataccg gtcccctgac ttcaccttga tatcctggac
     4621 ctgaattccc tggagcaggg tgaggggaac ctcgactcca ggctttccca gggcccagac
     4681 tttccggtag aaatccgcaa gcccattgac ctctttcttg tttacagtaa ggataatgtc
     4741 tccgactttg aggccgcctt tttccgcggg gctttccgaa gtgacccgag tcacaaaaac
     4801 ccgtccatga acctcttcta cgttcacccc taaccagggc ctcggagggg cagacgaccg
     4861 gcctttattg atcaagtcgg aaaggatggg tttcaggtaa ttgatgggca cgaacatgtt
     4921 ccctggaatc attcccagac cggggatgga gatctgggtg aaaagcgacc cgatccccac
     4981 cagttttcca ttgtggtcaa tcagggccgc ccctccgaag ttggcgtggg ggggagccgt
     5041 aaaaatggcg tcgtcaagga gatattccca gtagccggca aactcctttc gcgagataat
     5101 ccgggcgccc atggcccctt cttctccccc ataagtcgcc accaaaatcg ggtctccttc
     5161 tgtgacctcc gaagaatcgc ccagttgcat cggcttcgca tccatgggct tctccgcttt
     5221 caggattcca aatccggtta tatggtcgta agcaatcgat tgggcgctga tcgattttcc
     5281 atcgggggcg acaatttcaa gggtttcagc ttccaggatc aggtagccaa tggtgaggat
     5341 gtgtccctgg gtatctatga ggaccccgct cccttctctt tcggtcccga gagtcttggc
     5401 cgaacgggca taatccggaa ccacggcacg gaccttcacc accgctttga gaagctcttt
     5461 gacattctcc ttcgagccct cctgggcgta ggcggggaaa acc
//
LOCUS       DASPRR010000522        36899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1574547, whole genome shotgun sequence.
ACCESSION   DASPRR010000522 DASPRR010000000
VERSION     DASPRR010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 36899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36899)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36899
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574547"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VEL68_19425"
     CDS             <1..1428
                     /locus_tag="VEL68_19425"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXZ38206.1"
                     /translation="APSRFVPPGPFGIIEGDGCLNCAKCVKKSCAYQVYQKRSYDDRQ
                     LTDTADSLCKNCFRCVQECYGRVLSKSANPEYWSMGDEYWTPEIIGSNQRQAEAGKIP
                     VSGGGYGGPFAGPGFDSMWTDMSEIVRPTRDGIHGREYISTAVEFGPVSQPAAFMPNR
                     GSEERRAVSARLRVEVSVPILFDLLPFQEFPQPVLAALARAAKETNTLMILPAESIAG
                     ELSSWRAHLVPLLPPGNLGPFTPLIQQCRMVQITQDEKTGERIGEIKRANPAVIVSVR
                     VPLTPLAEEKIELLARAGVEVIHILADGQGREKEASRPRFIKDRIRDLHRALVEQGRR
                     DRVTLIFGGGIALAEHVAKAIICGADAVSIERPLLISLECRLCGQCSPKERCPVEINK
                     VETSWGRQRVVNLVAAWHGQLLEVLGAMGMRDVRRLRGEAGRAIFREDIEKETFADIF
                     GEKMQTRLVKAQPVQQGEIACAPNT"
     gene            1412..2944
                     /locus_tag="VEL68_19430"
     CDS             1412..2944
                     /locus_tag="VEL68_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXZ38207.1"
                     /translation="MPPTPEIKRAPSRYRNPIGKFKVQRDKGCTHCGLCAELCPRGVH
                     QRLGKKMLSPRDEWCIGPSCQKNEYYCIRRCPQGALRMAVNPNLQALRDHRWPADLLL
                     SNWQQAETGGVPYSDLEYRIGRSGGGFDRLRLRFPRIPPGGLPAEEEVSTALLLNRRE
                     DGRPRVRIGVPFYGGGMSFGSVSNHTMIARARAARAWDTFTCTGEGGYPESLKPYDSH
                     MITQVATGLFGVGEETIQRVRIVEFKYAQGAKPGLGGHLLGDKVTPAVARMREAVVGN
                     ALFSPFPFHSVYSVEDHKKHVDWIKEVNPRCLVSVKVSTPTDVDMVAVGSYYAGAHII
                     HLDGSYGGTGAAPDIAKKNIAMPIEYAIPKVHRFLVQEGIRNKVTLIASGGVRTAFDM
                     AKAIALGADGVVVGTADMVALECIRCRNCESGRGCPRAIATTDPEYSEQMNVEWAAQR
                     IINLYGSWVKQLVTILRLLGMRSITELVGRTDCLVHLDYQPEETDDDIKPYLMGSNGK
                     SA"
     gene            3019..5655
                     /locus_tag="VEL68_19435"
     CDS             3019..5655
                     /locus_tag="VEL68_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HXZ38208.1"
                     /translation="MKDQWENILKVLSSRAPLIQGLAEPPRWKAEEEGGCGVTGFACS
                     IPVGGRHIIEPSRQMNNRGNGKGGGIAAVGLCPESLGVSEEILEEDYLLQIAFLDPEA
                     RASVEGSFLSPFLEVHHSQRVPTVGDFRLVEGLEVKPPDAYRYFVRVKKEVLREFGAR
                     NQFNGMDPRKLEDEFIFQNSFQLNRHFYASLGEKKAFVLSHGRNMMILKIVGYAEQAA
                     QYYRLEDFPAHIWIAHQRYPTKGRVWHPGGAHPFIGLNEALVHNGDFANYHSVCEYLR
                     QHNLVPLFLTDTEVSVLLFDLLNRVMGYPLEYIIEALAPTTELDFDRLPPEKQKTYRE
                     VQAAHIHGSPDGPWFFIIARNDPVQRCHQLIGITDTAMLRPQVFALSDGEVRVGLICS
                     EKQAIDATLHSLSSEDSRFHRIADKYWNARGGSHTDGGAFIFTIREGGNGRPGKEMIC
                     TDKFGRPIAAPEYQRAWNHQPQPLAEQAREKLRREVREKFRGGDGLFLFENFKIEVPL
                     WDYDVFWEFGEELVQAANQGDSNRSTAIAALTYFLDRRVDPGEKRRSCLLEIALESLE
                     KVFSSVPGLDSNLGGIYRKIDWASQKSLRKPEGGEKTLILDAAQFPPEGDYGAARILT
                     QAYQLGWRHFIVYGCRGQRFIGCGLGPGTQGIRMDVYGSSGDYLGSGMDGMEIHVHGN
                     AQDQLGQVLKSGKLVIHGDVGQTFMYGAKGGTVYVLGNAAGRPLINAVGKPRVVINGT
                     ALDYLAESFMAGDPHNGGGFVVLNGMEFDERGMLREQSMPFPGSNLFSLASGGALYVR
                     DPHGRLEKEQLNGGEFSPLEEKDWKLILPHLQENERLFGIELKRDLLTVDGERKNPQE
                     VYRKVRAVKLSVLSATMPEDEV"
     gene            5878..6897
                     /locus_tag="VEL68_19440"
     CDS             5878..6897
                     /locus_tag="VEL68_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HXZ38209.1"
                     /translation="MNREGKAFSGEVIIRAITSLHERSNGLGGGFAGYGIYPDHKEDY
                     AFHVLYETEESRKLTKPALEGNYQLIQEEPIPTRQIDAVQNPPLVWRYFLRPRLERRI
                     SSVESEEDFVVRTVMEINQKKEGVFVASSGKNMGIFKGVGYPEEIADFYRLEEYRGYL
                     WTAHGRFPTNTPGWWGGAHPFGLLDWSVVHNGEISSYGTNRRYLKNFGYDCAFYTDTE
                     VVTYLFDLLHRKHQMAFPLVANILAAPLWDEIERMEDKEFYQFLRAVYGSALLNGPFA
                     VIAAHNQAMVGLNDRIKLRPLVAAEWQENLYIASEESAIREVCPDPDRVWAPEAGEPV
                     IGYLR"
     gene            6902..8425
                     /locus_tag="VEL68_19445"
     CDS             6902..8425
                     /locus_tag="VEL68_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXZ38210.1"
                     /translation="MAKGKITPEFKILRDMEKCVSCQVCHRVCSNDAHLYDRETGVVT
                     SEDSRCVGCHFCENLCPTGALGIRRNPSEIKLNALWSGRAVEDIARQSATGGVLLTGM
                     GNDRPFPVYFDHLLLNASQVTNPSIDPLREPMELKTYLGGKPDRLEVKEGQTREKVLK
                     SFLSSQIPLETPILFSAMSYGSISLNAVKSLAMAARESGTLWNTGEGGLHPDLYPFGP
                     NLVVQVASGRFGVHKDYLEAGAAIEIKIGQGAKPGIGGHLPGEKVTEEISRTRMIPEG
                     TDALSPAPHHDIYSIEDLKQLIYALKEAVRYRKPVGVKIAAVHNVAPIASGIVRAGAD
                     FVVIDGLRGGTGAAPRVIRDHVGIPIELAISVVDQRLREEGQRNRASLIAAGGFRNSA
                     DVVKAVALGADAVYVGTAALVALGCHVCQKCYTGKCNWGIATQDPYLTKRLNPQIGAR
                     RLVNLLRAWSLEIKEMLGGMGINALESLRGNREQLRAVGLGETDMRILGVKPAGEAW"
     gene            8427..8870
                     /locus_tag="VEL68_19450"
     CDS             8427..8870
                     /locus_tag="VEL68_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ38211.1"
                     /translation="MKKIFIHEEYCMNCRLCEVHCIVQNSKTRNIIKAYKGESPKPIS
                     RVLVEEQGYLSFALQCRHCEDAPCLDACITGAMHRDPDRGAVLADEERCVGCWMCIMV
                     CPFGVISRNVEGKRVISKCNLCQGEEIPVCVKNCPNEAIVFEEEK"
     gene            8867..10150
                     /locus_tag="VEL68_19455"
     CDS             8867..10150
                     /locus_tag="VEL68_19455"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ38212.1"
                     /translation="MRYVIVGNGPAGTAAVEGIREADPKGRITVISDEGVMNYSKPLL
                     SYLLARKVKKNQLAHRPGDFYARNGVELLLKKKAVKLETEQNRVVLSDGQSIPYDRLL
                     IATGGAPIPLKIPGKKTQGVFTFTRLEEAEELIHYLGDHSVREALVVGGGLIGLKATE
                     GLLGRGISVTLVELADRVLSATFDRRASEIIEGALKKEGCRVFLKNSVAKIAGDRSGR
                     VKEAVLRNQRKVACQVVIEAVGVLPEVEWLKGTEVRCDRGILVNEYLQTSAERVFAAG
                     DCCQTSGFFGGPPRVFPIWPSAVRQGKVAGFNMAGGKRKYDGPLVMNSVELCGIPTIS
                     VGLTDPQEGNGYEIVESVNEKKGVYKKLVLREDRIVGALFVGDISRAGVYTGLIKEAV
                     KTGDLKPHLLKENFGLIHLPKDYRKHYVTGEGIEV"
     gene            10147..10884
                     /locus_tag="VEL68_19460"
     CDS             10147..10884
                     /locus_tag="VEL68_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38213.1"
                     /translation="MKISAQGLHHRALNEKIHQAIEAGEREIFLTQVNGQRYIGDGIQ
                     NAGLQITIQGTPGNDLAAFMDGPSLFIYGNAQDGVGNTMNGGKIVIHGNAGDIAGYAM
                     RGGKIFIRGDAGYRVGIHMKAYNEFFPVIVIGGRAGNFLGEYMAGGLIVVLGLKVEMD
                     KSPVGTYVGTGMHAGKIYVRGSVRNYQLGKEIMHFDIDKNDREELEPLLHEYCENLNL
                     PWEEIRKGPFVKLSPYSHRPYGKLYSY"
     gene            11723..13135
                     /locus_tag="VEL68_19465"
     CDS             11723..13135
                     /locus_tag="VEL68_19465"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXZ38214.1"
                     /translation="MPPKGYNLDIEEAKTLKEIECLEKKDSRLWLLTIFMTLVLILFI
                     LAKDLWTLYLSPREFLEGFTSFRRSALIFPAALVLLSFCGYLIVQNRRIRRLRWKIFI
                     HKTKMERAMGSMEEMRALFQIASATADRKELPEILEIIARESLNCLKAHRSTVFSLDE
                     KSGILKPHFTFTSDPLNEQVGLFEEKEMARKSLRQRKGFLLREPADFTEYLKYLERER
                     KITSLLCIPLVSQDRSLGVLSVVMIDDDRKFNERDLQFLMVMGIQAAMADEKSSLDAE
                     LRRGASYRKEYEQYLDHILNQLQTLSDVERRRIEDHIKRLLPTPAGEERVPLSEPAEE
                     ARNGSPPETEPIALAQEVEDRVTKMLQVDIEGEPSSLAPDLGNGGVFIRTPNPLELGE
                     EFVLKLHMDEGETPVGVTCRVIWTNKYGKESRHLRRGMGVKFLDLSPQLRNRVESYIQ
                     SHRDNQFSLAEDQHHLSFGD"
     gene            13158..14312
                     /locus_tag="VEL68_19470"
     CDS             13158..14312
                     /locus_tag="VEL68_19470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ38215.1"
                     /translation="MGIFSFRAAGEITFGVGSVNRLGAAVRKAGGKKVLVVVDPGFAK
                     TGSFTRVTQSLEREGLSFVLFDQVEPEPRTVIADQGGEVARKERCDFVLGVGGGSAMD
                     TAKAAAVLATNGGQARDYQGLDKVPRPGLLKGMVPTTAGTGSEVTFTAVFINEEEKKK
                     AGINSPHLYPEMSILDPELTLSLPPAVTAFTGMDALAHAVESYTSKAAHPLSEMFSLE
                     GIHLIGKSLRRAVEKGSNLEARSDMLLGSLLAGIGLANAGVTAVHSLSYPLGGRFRVP
                     HGVGNGLLLPAVMEYNALSLPEKFARIAGAMGEKTEGLSAKNAALLAVESVKGLARDI
                     QVPMRLSDLGIPVSAFPGMAEEALKVTRPLENNPRPVSFEDAIQIYRSVF"
     gene            14461..15618
                     /locus_tag="VEL68_19475"
     CDS             14461..15618
                     /locus_tag="VEL68_19475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXZ38216.1"
                     /translation="MGGAELIGREEQEAVSEVIGRGVLFRYGFNEKRKNIFKVLEFEN
                     QFSKYMGCSYALGVSSGSAAVRVAVAALGIKRGDEVITQSFTFVATVEGVLEAGATPV
                     LTEVDASLNMDPKDLEAKITDKTRAIMPVHMLGVPARMDEILAIARKRNIPVIEDSCQ
                     ACGSGYKGKKTGTLGSLGTYSFDYVKTLTTGEGGMVVTNDKKVYELACEYHDHGHVHN
                     PEVPRGEDPHRITGFNYRMTELQGAFGLVQLNRLDSVLADQRKNKGEIKKAIAAIPQV
                     KFRDLPDPAGDGGDTLAFFLPDAKVAKAFNDHLAKSKVDTKILPSAMNWHFSGSWKHM
                     IQHLPPYRTDAWPKSEALLKRAIALPISVRMSDEQKARVVEAVHKAAKEVL"
     gene            15744..16484
                     /gene="kdsB"
                     /locus_tag="VEL68_19480"
     CDS             15744..16484
                     /gene="kdsB"
                     /locus_tag="VEL68_19480"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXZ38217.1"
                     /translation="MKVYGIIPARYGSTRLPGKVLADIGGKPMIQHVYERARRSPSLD
                     RLAVATDDDRVYQKVLDFGGEAVKTSPGHPSGTDRAAEAARVLGAQEKDLIVNIQGDQ
                     PLFEPGMVDEIVGPFRKDPDLLMGALVYPIRSREELLNPSVVKVVVDKLGFALYFSRS
                     AMPYVIESSMTPRYFKHIGPYAYRTGFLIQFTKIERGELEQAESLEQLRALENGYRIK
                     IVVTQYDSQEVDTPEDLERVRIRIAGVK"
     gene            16594..17886
                     /locus_tag="VEL68_19485"
     CDS             16594..17886
                     /locus_tag="VEL68_19485"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HXZ38218.1"
                     /translation="MFIFYNLLLLVFSVGAFPIFITKLVFSSQKRRVYAQKLGFLPWR
                     TLEKVKGQPRVWIHAVSLGEVGAVHPLIRELRRVYPDACLMLSTGTESGQKMARERLT
                     EANGTFFFPWDYPWVVRKVIRRLRPDLFVISETELWPNFLKIAKEEGAKTLLVNGRIS
                     DRSFRRYRKARFFFTAVLDNFDAMSMIRVQDGERIISMGANPVHVSVNGNCKFDQAAS
                     WALPGVQDEVRRILGVNPQDRVLVAGSTHEGEEEAVIKAFLRLRQSDPETLLILVPRH
                     IDRSDRVARLLERHGIREWVRRSQLNGEGRKGAKVVLWDTFGELFKVYSLATLVFCGG
                     SLVPKRGQNILEPAAWGKVVLYGPSMEDFLDAHQFLSSVGAGIMVQDEEELTDRCLYF
                     WGHPRELKERGEAGKEALWAQRGATQRNVELVRRLLGR"
     gene            17883..19094
                     /gene="carA"
                     /locus_tag="VEL68_19490"
     CDS             17883..19094
                     /gene="carA"
                     /locus_tag="VEL68_19490"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXZ38219.1"
                     /translation="MNRKKAVLALEDGKVFEGWSFGAPGEHAGEVVFNTSMTGYQEVL
                     TDPSYKGQIVTMTYPLIGNYGVNEEDLESTHPHVEGFVIKENCPFPSNWRSQGSLSDY
                     LDRFGIVGIEGVDTRALTKHLRTAGAMRGIISTEDLEPCSLIDKARTSPPMVGSDWVK
                     AVTCEAPYEWGRGEEGKKRVRGTGLGLWEWGRGQAILPSTGKKYRVVAMDFGMKHNII
                     RHLASRGTQVLVVPAKTQAEEILDLDPHGIFLSNGPGDPAALPAIIDTVRQLLGKRPI
                     FGICLGHQLLGLAMGGKTYKLKFGHRGANQPVKNLINGKVEITVQNHGFCVDLDSLPP
                     GQVQITHINLNDQSLEGMRHRWLPIFSVQYHPEASAGPHDAAYLFDEFISLMDRHSKE
                     SCPNEPTSKKS"
     gene            19061..22342
                     /gene="carB"
                     /locus_tag="VEL68_19495"
     CDS             19061..22342
                     /gene="carB"
                     /locus_tag="VEL68_19495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXZ38220.1"
                     /translation="MPKRTDIQKILIIGSGPIIIGQACEFDYSGTQACKALREEGFEV
                     VLVNSNPATIMTDPQMADRTYMEPVTPEVVAKIVERERPQALLPTLGGQTGLNTAIQV
                     DEMGILDRFGVEMIGANQEAIRKAEDRELFKEAMRRIGLEVPRSGFAHNLGEARDVVR
                     EIGLPVVIRPSFTLGGTGGGIAFNLEEFERLALQGIELSMIHEIMIEESVIGWKEYEL
                     EVMRDLKDNVVIICSIENFDPMGVHTGDSITVAPAQTLSDKEYQRMRDAAIAIIREIG
                     VETGGSNIQFALHPRDGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IPNDITRETMASFEPSIDYCVVKFPRWAFEKFRGADPTLTTSMKSVGEAMAVGRTFKE
                     ALQKAIRSLEIGRFGLGMDGKGEPDPDRMKEKLAHPTPERVFWLRAALKSGISPAEIA
                     ETTRIDQWFLENMKQVCDLEEEIQKIRLDGRGLESLPTYLLRQAKEWGYSDVQIAHLL
                     GSDEMEVRKRRKALGICPTFKLVDTCAAEFEAYTPYYYSTNESEDEVIPSDKKKVMIL
                     GGGPNRIGQGIEFDYCCVHASLALREEGFETIMVNSNPETVSTDYDTSDRLYFEPLTR
                     EDVLNIVEKEKPKGVIVQFGGQTPLNLAVPLEKAGVKILGTTPDSIDRAEDRERFKEL
                     IQKLGLVQPQNGTATSFEEARAVAREIGYPVVVRPSYVLGGRAMEIVYDEESLERYMR
                     EAVMASPERPILIDKFLEDAIEVDVDAISDGNRVVIGGIMEHIEEAGIHSGDSACVLP
                     PYTLSDDLMEVIKQNTYALAKELRVEGLMNIQYAIRDDRVYILEVNPRASRTIPFVSK
                     ATGVPLAKLAAKVMIGRTLEELGFTQEVEVSHVSVKEVVFPFNRFPGVDTVLGPEMKS
                     TGEVMGIDHAFGIAFAKAELAAGQVLPRTGKVFISVKNRDKRAIIFIAKKLSDFGFRI
                     VATRGTAKALSQNGIPVEVIHKVHEGRPNVVDAIKNRDINLIINTPTGRHPKKDQVSI
                     RAVAVAHNIPLITTISGAAASVHGIESLLQEEVRVKSLQEYHQKESPRVAAAKGAQTQ
                     PTTGD"
     gene            22348..23745
                     /gene="purF"
                     /locus_tag="VEL68_19500"
     CDS             22348..23745
                     /gene="purF"
                     /locus_tag="VEL68_19500"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXZ38221.1"
                     /translation="MKTIDCPKEECGIFGIFDHPEASHLTYLGLYSLQHRGQESAGIV
                     TGYEGEPKTHKGMGLVADVFDQNTLKALCGNSAIGHVRYSTTGSSLLKNAQPFLAQRQ
                     KSFMAVAHNGNLVNARQLKDRLEAQGSIFQSTMDSEIFVHLIARSRRKGYQERTIQAL
                     SQVQGAFSLILLTENGLIGARDPHGFRPLCLGKLDGSFVLASETCALDLVQAKYISDI
                     HPGEVVMIDKHGLHSEKPFPRVKPSFCIFEYIYFARPDSNIFGENVYLVRKKLGARLA
                     EEAPAEADFVMPIPDSGNYAALGFAERLRLPFEMGMIRNHYVGRTFIQPSQSVRDLGV
                     KVKLNPVKDLLRGKRVVVVEDSIVRGTTSRTRIRALRDAGVKEVHMRVSCPPHRFSCH
                     YGIDFPTREELIGSSRSVEEIRKFIGLDSLAYLSVEGMVGAMPQSRDSFCLACFTGKY
                     PVPFSPRTTKLALEQ"
     gene            24093..24707
                     /locus_tag="VEL68_19505"
     CDS             24093..24707
                     /locus_tag="VEL68_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXZ38222.1"
                     /translation="MKDESKHQKILQAAVKVFSEKGFYNSRVSEIAKEANVADGTIYL
                     YFKNKDDILISLFEEEFGKIVQNMRAALEKDKDALQKIKRFAITHLSIVTKQQELAEV
                     MGVEVRQSSKFMKEYVNKPFIEYLNIIRSVVIEGQEKGLIRKDLTPGIMKRAFFGALD
                     EMARYWVLSTQKKHSTDDAALQISDVFIRGMMSEEARRNYTWPS"
     gene            24947..25738
                     /locus_tag="VEL68_19510"
     CDS             24947..25738
                     /locus_tag="VEL68_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXZ38223.1"
                     /translation="MNVIVCMKQVPDTEALIKVKPDGSGIVTDDIKYVMNPYCEFAVE
                     ESLRIKEKQGGASILVTLGPARAVEALRTGLAMGSDRSVHLNDPAFEGGDGFATAKVL
                     AEAIKKEPYDVILCGKQAVDDDMAQVGQSLAELLNLPHITLITKLEFSADKKKAKVER
                     EVDGGKEVIEVNLPAVFTCQKGLNEPRYASLPGIMKAKKKEVKAINLAALGIPADQTG
                     AAGSKIKVLKYSSPPSRPPGKIVSGELLEAAQKLVKLLREEAKVI"
     gene            25818..26810
                     /locus_tag="VEL68_19515"
     CDS             25818..26810
                     /locus_tag="VEL68_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXZ38224.1"
                     /translation="MAKGIWVFSEVKDNNIRKVTFELLSQGRKMAGKLGEEVVAVLLG
                     SGVEGLTGRLAEFADKVFWVDAPALAQYTTDAYSSVLTRLLKEHQPSIFLCGATVIGK
                     DLSPRLATRLQTGLATDCISLSIGDDGAMVAKRPVYAGKAYIEVTYPVARPQMASVRP
                     NVLEMVQPDGTKKGSIIKVEAQIDAGSLRTTVLEVVKSAAAKVDLTEAEIIVSGGRGM
                     KGSENFKILEELAEVIKATVGASRAAVDSGWREHGDQVGQTGKVVTPNLYIACGISGA
                     IQHLAGMGSSKVIVAINKDPDAPIFQKADYGIVEDLFKAVPVLTEEFKKLLSES"
     gene            26970..28934
                     /locus_tag="VEL68_19520"
     CDS             26970..28934
                     /locus_tag="VEL68_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HXZ38225.1"
                     /translation="MLSSFDLFLLFIVAVVFAYGLYRRYRLWMIGKPEKRIDRPKERL
                     ISLWAYVAGHKRMLRDVYPGWMHLLIFYGFLVPFVVVAITQANFSLPRALSLALSLFF
                     DLVGTLGIVGLLMAAYRRYVQKPDNLTYDTYWGNAVALIFLLAIFGLGFCVEGLRIAR
                     TQPDWVAWSPVGWLFSQFFSGLTDIHQVLLHRILWRLHLFLVLGFIAFIPYWRMLHIV
                     TGPANVYMRSLTPKGELPPIRDFETAETFGASKISELTRKQLFDLDACTRCGRCLDHC
                     PGSVSGKPLAPKKNWDTLRAHLEEKAALMRKGVDPYAEGQTKLIGSGTGDGVSEDVIW
                     ACTNCLACAMVCPVFIPCVDKFLEMRRYLVLMESNFPQEAQLVFRNMENNSNPWGVGS
                     GLRADWMKGLEVKTMAENGDIDLLFFVGCAGSFDDRNKKVATSLVKILNACGVKFSVL
                     GTEEGCCGDSARRIGNEYLYQTLVQTNIEVFKNYNIRKIMTMCPHCFNTLKNEYPQFG
                     GNYEVIHYTHFLAEALGSGRLKLKKSLEKVITYHDSCYLGRANEVYEEPRRIIQAIPG
                     LKLVEMERNHIRSFCCGAGGGRMWMEEKLGTRINQLRTDQALQTKANWVGTACPYCLT
                     MLGDGIKEKGLEESMAAHDLSELVVQAMDS"
     gene            29028..29912
                     /locus_tag="VEL68_19525"
     CDS             29028..29912
                     /locus_tag="VEL68_19525"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXZ38226.1"
                     /translation="MVRNDKRCIGECIRTLFPFLAFFLFLACAAHAAEDAECLKCHKN
                     PRLSKGNKDGSLLSLYVKEEAFKASVHGAAGMGCTDCHQEAKPNVHPAEGFPEVGCAN
                     CHQDSAEAYKQTAHGMMLESGMERAPKCQDCHTSHYTRKIADPQSPVKASRLPQACAK
                     CHEAAQVPKGFLMALATYRIMGHPKTSLEYSYDTQGCANCHPDNAGHAQKKGPPPSCV
                     KCHDPSFSKPLLLGPIHFKVSLGDQPVPFLLRFLYGAGLILVVLACIAFFGYRTYTRR
                     KARKPAGEKPTEGQGTTS"
     gene            30127..31269
                     /locus_tag="VEL68_19530"
     CDS             30127..31269
                     /locus_tag="VEL68_19530"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ38227.1"
                     /translation="MDFQLSPQQELLRKSVREFAEGAVAPKVTHMEETDEVPWDLYKE
                     MSKQLYMGVLIPKEFGGTAFGHLARMIMLEEVGRVSAATAMALQIFHLGTVPIVDFGN
                     EAQKKKYLPGMAKGERLSTIAVTEATGGSDPTGIQTTAKLQGDSYIFNGRKCFITHGH
                     VADVLTIMAKTGEGPKGFSAFIVEKSFPGFKLGRKEKKFGLHGCNTGEIAMENCAVPR
                     GNLLGSEGDGLKVSMAAISEVGRAGMAGCGLGVINACLEASVKFANERILGGKPISQH
                     QAIQWMISDIYMDLEASRLLAYRASWLKDQKVRCDTDMAMAKYYATEAAVRCAKKAVD
                     IHGGYGYMMEYPVQRYYRDAEILIASAGTSEIQRIVMARKALTSFK"
     gene            31444..32235
                     /locus_tag="VEL68_19535"
     CDS             31444..32235
                     /locus_tag="VEL68_19535"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXZ38228.1"
                     /translation="MNIVVCVKPAPDPQRWDSIQLDPKTKALHRDGIPSVLGPLDRRA
                     LEEGLRIREKLGGKVVAMAMAPPSARDNLVEALAMGADEAVILSDRAFAGSDTWATSF
                     VLARGVQRSGAFDLVLCGEWSLDGSTGHVGTQLAEYLGIPNASRVAEVESLEGGTLRT
                     RTMIEGGYRILETPLPALITVTRDINTPRFTSLLGVMEAEMKPLVIWSAAELGIRPEE
                     VGFSGSPTQTGDVFMPEMKRKGEVLQGEPEEMVKEIIRKVRQALG"
     gene            32366..33373
                     /locus_tag="VEL68_19540"
     CDS             32366..33373
                     /locus_tag="VEL68_19540"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXZ38229.1"
                     /translation="MKEWKGVWVFVQQEEGCVAEVSFELLTAARELSSKRGNPEVVAL
                     LLGYEVKGLAKDLLSYGADRVLCADHPSLGKYLTLPYTRVIGRLISERKPDIFLLPAT
                     SVGADLAGRVAIRVNTGLSAHCTHLDINAGGELMQVVPAFGGKVMATILCPKHRPQMA
                     TIRPGVYRRGELRKGRGEVEQVSPDIQPEDLGQRVVETHWEKKPAQAVEEAEVVVAGG
                     AGIGNAEDWKWVERLAQALHAAVGGTRPPLDEGWIKEGQMIGQSGKTIRPKLYIGVGI
                     SGVIQHVVGIQDSKVIIAINNDPHAAIFQSADLGAVTDFRRIVPLLVQELQKKNEPLY
                     R"
     gene            33450..34070
                     /locus_tag="VEL68_19545"
     CDS             33450..34070
                     /locus_tag="VEL68_19545"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXZ38230.1"
                     /translation="MKIDPANLDRKSAHEIMVGSALPRPIAFVSTIGPDGVYNLAPFS
                     FFIPMSVKPAIVGVGIGRKGDGKKKDTLVNIEFSKEFVINLVTEEIAEAMNQASGEYP
                     SHVDEFKEAGLTPVKADLLKSPRVAESPVNMECRLLQALEFGEFPRINTFVIGEVIRI
                     HVKDEFWRNGEILSSKLKAIGRMGGDLYCRTREVFEMKRPVIEERW"
     gene            complement(34164..34742)
                     /locus_tag="VEL68_19550"
     CDS             complement(34164..34742)
                     /locus_tag="VEL68_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HXZ38231.1"
                     /translation="MPYAAKDYGKLIGMEGFSETLLKNHFTLYQGYVTNTNKLMDTLG
                     QMLKEGKTATPEFAELKRRFGWEFNGMRLHEYYFENLGGKGAIDKGGKLFKKIAESFG
                     NYEEWEKDFRGVGTMRGIGWAILYQDSVSGALCNCWINEHDVGHLAGCNPILILDVFE
                     HAFMIDYGLKRPDYIASFFKNVEWKAAEARVK"
     gene            complement(35000..35599)
                     /locus_tag="VEL68_19555"
     CDS             complement(35000..35599)
                     /locus_tag="VEL68_19555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012186381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXZ38232.1"
                     /translation="MQVSVPDSRVYERLPLPAKKTALLLSDMLVDFVSPKGKLFVPDT
                     RKTIAPIRSLLGKARKSRVMIVYIQDWHRPDDPEFSIWGPHAIQETTGGRIIPELSPA
                     PKDFLVRKRTYDPFFSTDLDLLLRQKGIKNLVITGTVANICVLHAAGSATLRGYQVIV
                     PIDTISALNPFDFQAALRQVSFVYQGKLTKSEGISFQKK"
     gene            complement(35850..36740)
                     /locus_tag="VEL68_19560"
     CDS             complement(35850..36740)
                     /locus_tag="VEL68_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HXZ38233.1"
                     /translation="MKSQKRVIFAKDPEKFIEKAIVHFIQTSPANRRKEGRGRYWGRP
                     LVGFASGEDPLFKAYKKIIGQFHFSPQEIFRLTFGNKKPPQPLSIIAWILPHVEEIRL
                     SNRKETKYPSRLWAHARDFGEQFNVKLRKHVVSFLKKKGYQAVAPMISPHWRRVRSPR
                     IGLASSWSERHTAYACGLGSFGLSDGFITTRGKAIRIGSVVTNLPLKPSAKKYPHHRA
                     NCLYFFNRTCKVCALRCPAGAITSRGHDKDKCQEYSYNTIGPLKRGEYGVKITGCGLC
                     QTKVPCEYEIPKRIQKSYKR"
BASE COUNT         8452 a         9838 c        10945 g         7664 t
ORIGIN      
        1 gccccctcgc gattcgtccc tccggggccc tttgggatca tcgaagggga tggctgccta
       61 aactgtgcca agtgtgtcaa aaaatcttgc gcctatcagg tctatcaaaa gaggtcgtac
      121 gatgaccggc agttgacgga cacggcggac tcgctgtgca aaaactgctt ccgttgtgtt
      181 caagaatgct acggccgggt cctctccaaa agtgcaaacc cagaatactg gtccatgggg
      241 gacgaatact ggacgccgga gatcatcgga agcaaccaac gccaggcgga ggcgggcaag
      301 attccggttt cagggggagg atacggaggc ccctttgccg gacccgggtt cgacagcatg
      361 tggaccgata tgtccgagat cgtccgcccc acccgcgatg ggatccacgg ccgggaatac
      421 atcagtacgg cagtggagtt cggccccgtt tcccagcccg cagcctttat gcccaatcga
      481 gggtccgagg agcggcgagc cgtctccgcc cgcctgcggg tggaggtctc cgttcctatc
      541 cttttcgatc ttctgccttt ccaggaattc ccccagcccg tcttggcggc gctggcccgg
      601 gcagccaaag agacgaacac gctcatgatt ctcccggctg agtcgattgc aggagagctg
      661 tcctcctgga gggctcacct ggtccccttg ctccccccgg gaaacctagg cccgtttact
      721 cctctgattc agcaatgccg aatggttcaa atcacccagg acgaaaaaac cggggaaagg
      781 attggcgaga tcaagagagc caatcccgcg gtgattgtgt cagtccgggt tcccctgacg
      841 cctctggcgg aggagaaaat cgaacttctg gcccgggcag gcgtcgaagt gatccatatt
      901 ctggccgatg ggcagggacg ggagaaggag gcctcgcgac cgaggttcat caaggaccgc
      961 atacgagacc ttcaccgcgc cttggtcgaa caagggaggc gggaccgggt caccctcatt
     1021 tttgggggcg ggatcgccct ggccgagcat gtggccaagg cgatcatctg cggggcggac
     1081 gcagtgtcca ttgaaaggcc gcttctcata tccttggaat gccggctatg cggtcaatgc
     1141 tcgccgaagg agcgttgccc ggtagagatc aataaggttg agaccagctg gggaaggcag
     1201 agggtggtca atctggtggc tgcctggcat ggtcaactcc tggaagttct gggagccatg
     1261 ggcatgaggg acgtgcgcag gctccggggg gaagcgggcc gcgcgatctt tcgggaggac
     1321 atcgaaaaag agaccttcgc cgatatcttc ggagagaaaa tgcagacccg cctggtgaag
     1381 gctcaacccg tccagcaagg agaaatcgcc tgtgccccca acacctgaaa tcaagcgggc
     1441 cccctcccgg tatcgaaacc ccattgggaa gttcaaggtc caaagggata agggatgcac
     1501 ccattgtggc ctctgcgccg aattatgccc ccggggggtc catcagcgct tgggcaagaa
     1561 gatgctctcc ccgcgggacg aatggtgcat cgggccttcc tgccagaaga atgaatatta
     1621 ctgtatccgc cgctgccccc agggagccct tcggatggcg gtcaacccca acctgcaggc
     1681 cctgcgggac catcgctggc ccgcagacct gctcctttcc aattggcagc aggccgagac
     1741 cggaggggtt ccttactccg acttggagta ccggatcggg aggtccggag gagggttcga
     1801 ccggttgcgg ctgcggttcc cccggattcc cccgggggga ttgccggccg aagaagaagt
     1861 ctccacagct cttctcctaa accgccggga agacggaagg ccccgagtac ggatcggggt
     1921 tcccttctac ggagggggga tgtcttttgg ttcggtgagc aatcacacca tgattgcccg
     1981 agccagagcg gcaagggctt gggatacctt cacctgcacg ggagagggtg gatatccgga
     2041 atcgctgaag ccttatgact cccacatgat tacccaggtc gccaccggac tcttcggggt
     2101 gggggaagag accatccagc gggtaagaat cgtggaattc aagtatgccc agggagccaa
     2161 acccggcctg ggaggtcatc tcctgggaga caaggtcacc ccagccgtgg cccggatgcg
     2221 cgaggcggtg gtgggaaacg ccctgttctc tccctttccc ttccacagcg tctattcggt
     2281 ggaggaccat aagaagcacg tagactggat caaggaagtg aatccacgct gtctggtgtc
     2341 ggtgaaagtt tccaccccca ccgacgtgga catggtggcc gtgggcagtt actatgcggg
     2401 ggcccacatc atccatcttg acggaagcta cggggggacg ggggccgcgc cggatatcgc
     2461 caagaagaac atcgccatgc ccatcgaata cgcgatcccc aaagtgcacc gctttctggt
     2521 gcaggaaggg atccgcaaca aggtcaccct gattgccagc gggggggtgc gtacggcctt
     2581 tgacatggcc aaggccatcg ccctgggcgc ggacggggtg gtggtgggaa cggccgatat
     2641 ggtggccctg gaatgcatcc gctgccgaaa ctgtgaaagc ggccggggat gcccccgggc
     2701 cattgccacg accgatccgg aatattccga acagatgaat gtggaatggg cggcccagcg
     2761 gatcatcaat ctctacgggt cgtgggtgaa acagctggtg accatcctgc ggctgctggg
     2821 catgcgttcc attaccgaac tggtgggacg gaccgactgc ctcgtccact tggactatca
     2881 gccggaagaa accgatgatg acattaaacc ctacctgatg ggaagcaacg gaaagtccgc
     2941 atagagaaaa gcgctacact tttaggcggt ctggccttgg aatcatgtcc tgaacgaacg
     3001 catgaacagg gtccattcat gaaagatcaa tgggaaaaca tcctgaaagt cctttcttcc
     3061 agggcgccgt tgattcaggg gcttgctgaa ccccctcgtt ggaaagctga agaagagggc
     3121 ggctgcggag taaccggctt tgcctgttcc attccggtcg gcggcaggca catcatcgag
     3181 ccctcccggc agatgaacaa ccgggggaat ggaaagggag gaggaatcgc tgccgtcggc
     3241 ctttgcccgg aaagtctggg agtctccgag gaaattttgg aagaagatta tctgctccaa
     3301 atcgccttcc tcgacccgga ggctcgagcc tcagtggagg gaagtttcct ctctcctttc
     3361 ttggaagtcc accattccca gcgggtgccc accgtcggcg actttcggct ggtggagggt
     3421 ctggaagtga agcccccaga tgcctaccgc tactttgtcc gggtcaagaa ggaggtcctc
     3481 cgggaattcg gggcccgcaa ccagtttaac ggaatggacc cgcgaaagtt ggaggatgaa
     3541 ttcatcttcc agaacagctt tcaattgaac cgccatttct acgcctccct gggggagaag
     3601 aaagcctttg tcctctccca cggcagaaac atgatgatcc taaaaatcgt gggctacgcg
     3661 gaacaggccg ctcagtatta tcggctggaa gatttcccgg cccacatctg gatcgcccac
     3721 cagcgctacc cgaccaaagg tcgtgtctgg cacccagggg gagcccaccc attcatcgga
     3781 ctaaacgaag ccctggtgca caacggggat ttcgcgaact atcactcggt ttgcgaatac
     3841 cttcgacagc acaacctggt gcccctgttc cttacggata cggaagtctc ggtcttgctc
     3901 ttcgacctgc tcaaccgggt catgggctat cccctggaat atattatcga ggccctggcg
     3961 cccaccaccg agctggactt tgatcgcttg cccccggaaa agcaaaagac ctaccgcgag
     4021 gtccaggccg cccacatcca tgggtctccc gatggcccct ggtttttcat cattgcccga
     4081 aatgaccctg ttcagaggtg ccatcaactc atcgggataa ccgacaccgc catgttgcgg
     4141 ccccaggtgt ttgccctctc ggacggggag gtccgggtgg gattgatctg ctcggagaaa
     4201 caggccattg acgccaccct ccacagcctg agttccgagg attcccgctt ccaccggatt
     4261 gccgacaaat actggaatgc caggggagga agccatacgg acggaggggc cttcatcttt
     4321 acgatccggg aggggggaaa cggccgtccg ggaaaagaaa tgatttgtac cgacaagttc
     4381 gggaggccga ttgcggcccc tgaatatcaa agagcctgga accaccaacc gcaacctctc
     4441 gcggaacagg cacgcgaaaa gctgcgcagg gaagtcaggg agaaattccg gggcggggat
     4501 ggcctttttc tcttcgaaaa ttttaagatt gaggtccccc tgtgggatta cgatgttttc
     4561 tgggaattcg gggaagagct ggttcaggcc gccaaccagg gcgattccaa ccggtccacg
     4621 gccatagccg cgctgaccta ttttctggac cgtcgggtcg atcccgggga aaagcggaga
     4681 agctgccttc tcgaaatcgc gctggagagc ctggagaagg tcttttcttc ggtccccggg
     4741 ctggactcaa acctcggggg aatctatcgg aaaatcgact gggcttccca gaagtcccta
     4801 cgaaagcccg aggggggaga gaagaccctc atcctcgacg ccgcccagtt tcccccggaa
     4861 ggcgattacg gggccgcccg aatcctcacc caggcttatc agctcggctg gcgtcatttc
     4921 attgtttatg ggtgccgggg ccagcgcttc atcgggtgcg gcttggggcc tggaacccag
     4981 ggaatccgga tggatgtgta tggaagctcg ggggactacc tgggatcggg gatggatggc
     5041 atggaaatac acgtccatgg aaacgcccag gatcaattgg gccaggtctt gaagtcgggg
     5101 aagctggtca tccatggaga tgtgggacag acctttatgt atggggctaa aggtggaacg
     5161 gtctatgtgt tgggaaacgc ggccggaagg cccttgatca atgccgtagg gaagccgcgg
     5221 gtggttatca acggaacggc cctggattat ctggccgagt ccttcatggc cggggacccc
     5281 cacaacgggg gcggttttgt ggtcctgaac ggaatggagt ttgacgaaag aggcatgctg
     5341 cgcgagcaat ccatgccctt cccggggtcc aacctttttt ccctcgcttc cgggggagcc
     5401 ctttatgtgc gggaccctca tgggcggctg gaaaaggaac aattgaacgg cggcgagttt
     5461 tccccattgg aggagaagga ttggaaactc atccttcccc atctccagga gaatgaaagg
     5521 ctctttggca ttgaattaaa gagggacctg ctgaccgtcg acggggagag gaaaaaccct
     5581 caagaggtat accgcaaggt ccgggcggta aaattgtccg tgctgtcggc gacaatgccc
     5641 gaagacgagg tttagccgca aattcgaagg gtcccatgca cgctgctggc cttcctgagt
     5701 tgcaagaacg acatgatcca ccgcattccc tttcctaaga ataggctata atcatatagt
     5761 agtaaaacct gatgaatggt agctaccttc ggggatcaaa gggtctgaaa ggagaaagcc
     5821 attttagtcg ccgatcgaga ttaccggatt ccctccgcct gcggcctagt ggggatcatg
     5881 aaccgggagg gtaaggcgtt ttccggcgaa gtgatcatcc gggctatcac tagcctgcac
     5941 gagcggtcca atgggctcgg gggaggcttt gccggctacg ggatttaccc ggaccacaag
     6001 gaagattacg ccttccatgt cctgtacgag actgaggaat cccgaaaact gaccaaaccg
     6061 gctctggagg ggaactatca actgatccag gaagagccca ttccaacccg gcagattgac
     6121 gcggtccaga atcctcccct cgtgtggcgg tatttcctgc ggccccgatt ggagcgtcgc
     6181 atctccagcg tggaatcgga ggaggacttt gtcgtgcgca cggtcatgga gatcaaccag
     6241 aaaaaagaag gagtcttcgt cgcttcttcc gggaagaaca tggggatctt caagggagtg
     6301 ggatacccag aggagatcgc ggacttttac cggctggagg agtaccgggg gtacctgtgg
     6361 accgctcacg ggcggtttcc caccaacacc cccgggtggt ggggaggagc ccatcccttc
     6421 gggctcctgg attggtcggt ggttcacaac ggggaaattt cctcctacgg caccaaccgt
     6481 cggtacctca agaatttcgg gtacgactgc gccttctaca ccgacacaga ggtggtcacg
     6541 tacctcttcg acctgctgca ccgcaaacac cagatggctt tccccctggt agccaatatc
     6601 ttggccgctc cgctctggga tgagatcgaa aggatggagg ataaggaatt ttaccagttt
     6661 cttagggcgg tttacgggag tgccctgctg aacgggccct tcgcggtgat tgccgcccac
     6721 aaccaggcca tggtggggct gaatgaccgc atcaaattgc ggccgctggt ggccgccgaa
     6781 tggcaggaga atctgtatat cgcttcggaa gaatcggcca tccgggaagt ctgtccggac
     6841 cccgaccggg tctgggcccc cgaggcgggg gagccggtca tcggctactt gaggtgaaaa
     6901 attggccaaa ggaaagatca ccccagaatt taagattctg cgggatatgg aaaagtgcgt
     6961 aagctgccag gtctgccacc gggtatgctc caatgacgcc cacctttacg accgggaaac
     7021 cggggtggtg acctcggagg acagccgctg cgtaggctgc catttctgcg aaaacctctg
     7081 cccaacgggg gccctgggca tccgccgcaa tccttcggag atcaagctga acgccctctg
     7141 gtccggaagg gccgttgagg atatcgcccg ccagtccgcg accggagggg ttctcttgac
     7201 cgggatgggc aacgaccgac cgttcccggt ttatttcgac cacctcctgc tgaatgcttc
     7261 gcaggtcacc aatccatcca tcgacccgct gcgggagccc atggaactga agacctacct
     7321 gggggggaaa ccagatcgcc tggaagtgaa ggagggacag acgcgggaga aagtcttgaa
     7381 atccttcctc tcctcgcaga tccccctgga gaccccgatc cttttctccg ccatgtccta
     7441 cgggtccatc agcctcaatg cggtcaagtc cctagccatg gcggccaggg aatcggggac
     7501 cctctggaac acgggggaag gagggttgca cccggacctc tatcccttcg gcccgaacct
     7561 ggtagtgcaa gtggcttccg ggcgcttcgg ggtgcataag gattacctgg aggcgggggc
     7621 ggccatcgag atcaaaatcg ggcagggggc gaagcctgga atcgggggcc accttccggg
     7681 ggaaaaagtc accgaagaga tctcgcgcac ccgtatgatc cccgagggga ccgatgccct
     7741 ttctccggcc ccccatcatg acatctactc tatcgaggac ttgaaacagc tgatttacgc
     7801 gctgaaagaa gcggtgcgct accgcaaacc cgtgggggtc aagatcgccg ccgtccataa
     7861 tgttgcgccc atcgcctccg ggattgtccg ggccggcgcg gatttcgtgg tcatcgacgg
     7921 cctgcgcgga ggaaccggcg cggccccccg ggtcattcgc gaccatgtgg gaattcccat
     7981 cgaactggcc atttcggtgg tcgatcaacg gctgagggaa gagggccagc ggaaccgggc
     8041 ctccctgatc gccgccgggg ggttccgcaa cagcgccgat gtggtcaagg ccgtcgccct
     8101 gggcgcggac gcggtctacg tggggaccgc cgcgcttgtg gccctggggt gccacgtctg
     8161 ccagaaatgc tatacgggaa agtgcaactg ggggatcgcc actcaggacc cctatctgac
     8221 caagcgcctg aacccgcaga tcggagcccg gcggctggtc aatctgctgc gggcctggtc
     8281 cctggagatc aaggagatgc tggggggaat ggggatcaac gcactggaga gcctgcgggg
     8341 caaccgggaa caattgcgcg ccgtgggcct gggggagacg gatatgagga tcctgggggt
     8401 caaacccgcg ggggaggctt ggtaggatga agaagatctt tatccatgag gaatactgca
     8461 tgaactgccg cctctgcgaa gtgcactgca tcgtccagaa ttccaagact cgcaacatca
     8521 tcaaggctta taagggagaa tcccccaagc ccatttctcg ggtgctggtg gaagagcaag
     8581 ggtacctgtc cttcgccctc cagtgccgcc attgcgaaga cgctccctgc ctggatgcat
     8641 gcatcaccgg ggccatgcat cgggacccgg atcgtggagc ggtcctggcc gacgaggaac
     8701 ggtgcgtggg gtgctggatg tgcatcatgg tctgtccttt cggagtcatc tcccgaaacg
     8761 tggaaggcaa acgggtgatc tccaaatgca acctttgcca gggggaagaa atcccggtgt
     8821 gcgtgaagaa ctgccccaac gaagccatcg tctttgagga agaaaaatga gatacgtcat
     8881 cgttggaaac ggccctgccg ggaccgccgc cgtggaggga atccgggaag cggacccgaa
     8941 ggggcgaatt acggtgattt ccgatgaagg ggtgatgaat tattccaaac cccttctctc
     9001 ctacctgctg gcccgaaagg tgaaaaagaa tcaactggcc catcgtccag gagattttta
     9061 tgcccggaac ggagtggagc ttttgctgaa aaaaaaggcg gtgaagctgg agaccgagca
     9121 gaaccgggtg gttctgtccg acggccagtc cataccctat gaccggctcc taattgccac
     9181 cggcggtgca cccatccccc ttaaaattcc cggaaagaag acccaggggg tctttacctt
     9241 cacccgcctg gaagaagccg aagaactcat ccactacctg ggggaccatt cagttaggga
     9301 ggcgctcgtg gtgggagggg gattgatcgg cctgaaagcc acggaggggc ttttggggag
     9361 agggatctcg gtgaccctgg tggagctcgc cgaccgggtc ttatccgcta catttgaccg
     9421 ccgggcatcg gaaatcatcg aaggggctct caagaaggaa ggctgccggg tattcttgaa
     9481 gaacagcgtg gccaagatcg cgggggatcg gtcgggcagg gtgaaagaag cggttttacg
     9541 gaatcagcgc aaggtcgcct gtcaagtggt catagaggct gtgggggtcc tgccggaggt
     9601 ggaatggctc aaaggaacgg aggtccgctg cgaccgggga attctggtga acgaatacct
     9661 ccaaacctcg gccgaaaggg tttttgccgc cggggactgt tgccagacat ccggtttttt
     9721 cggagggcct ccccgggtgt tccccatatg gcctagcgcc gtccgtcagg gaaaggtggc
     9781 cggtttcaac atggccgggg ggaagagaaa atatgacggg cccctcgtca tgaactcggt
     9841 cgaactctgc ggcatcccca cgatctcagt gggcctcacc gacccccagg aggggaacgg
     9901 gtatgagatc gtcgaatcgg tgaatgagaa aaaaggggtc tacaaaaagc tggtcctgcg
     9961 ggaggaccgg attgtgggag cccttttcgt gggggatata agccgggccg gtgtttacac
    10021 cggattgatc aaggaggcgg tgaagaccgg ggacctgaag ccccacctgt tgaaggagaa
    10081 ctttggcctc attcaccttc ccaaagacta tcgcaagcat tacgtcaccg gagaaggaat
    10141 cgaggtatga agatttcagc ccaaggcctg caccaccggg ccctgaacga aaaaatccat
    10201 caggcaatcg aggccgggga aagggaaatt tttctcactc aggtcaacgg tcaacgttac
    10261 attggagacg gaattcagaa tgcaggcctt cagatcacca tccaggggac tccgggaaat
    10321 gacctggccg cctttatgga tgggccttcc ctgtttattt atgggaacgc gcaggatggg
    10381 gttggaaaca caatgaacgg gggcaaaatt gtcattcacg ggaatgcggg cgatattgcc
    10441 gggtacgcca tgcggggggg caaaatcttc atccgcggtg atgccggata ccgggttgga
    10501 attcacatga aggcctacaa tgaatttttt ccagtcatcg tgatcggcgg ccgggcggga
    10561 aatttcctgg gagaatacat ggccggagga ctgatcgtag tcttggggct caaggtagag
    10621 atggataaat cgccggtcgg cacctatgtg gggaccggga tgcatgcggg caagatctat
    10681 gtccggggga gcgtgcgtaa ctaccagctg ggtaaggaga tcatgcattt cgacatcgat
    10741 aaaaacgacc gggaagagct cgaacccctc ctccacgaat actgtgagaa ccttaacctg
    10801 ccgtgggaag aaatccggaa ggggcctttc gtgaaactaa gcccttactc ccatcgcccc
    10861 tacgggaaat tgtactccta ctaaaagaag gggggatgaa caataaccat taaccagtga
    10921 tcattggcca ttgaaaacgg attcttagct gaacccgccc ctatccgata aggcgacaaa
    10981 cctgccggta cattttccgc ctttctccct ttgccttcca ttttttttga cactgctcct
    11041 cagttcaatt atattaagat atgaaggacc tcttgaatat cgggaaacgc tggtgatgaa
    11101 gttgagcggt aggatcatcc gctagattcg agagtgggta aagcaagatg gagcggaaaa
    11161 aggagtccag agtttcccaa agtagagtga accatgggaa agatgatgag gatgacccaa
    11221 agccggcata aacgctccat tccaccgatt ttccgtttaa aatcatctag atttccagct
    11281 aaagggaaaa gaaagagaag ccgataaaag gatagaggga aaaggaactt ttccccgaac
    11341 ggctccccat gcggttcgag gaatagcggc agggaaaaag caggttacct gagcggctgg
    11401 agagaaaagg gactccatcg ttttttcccc ttgcccgaaa gagatggttt aaagcacact
    11461 cacagttttc acggggaaga aacccctctg ccggaacctc cggtcctatc atgcaaacct
    11521 ccaatcgacc caggttttac aactgggata cgcttgacgt tccgtcaggc ggataatatt
    11581 cataaggggg accctttacc tcccttgggg attcatgact ggtcgaattt tacccattcc
    11641 tgataaagaa agaagctccg gcaaggggga cgagaaacca caggtctcca aaaaggctga
    11701 gaggcaaatc ctccaacctg agatgccccc caagggttac aaccttgata tcgaagaagc
    11761 caagacctta aaggaaatcg agtgtctaga aaaaaaggac tcccgcctct ggcttctaac
    11821 gatcttcatg accctggtgt tgatcctatt catcctggcc aaagaccttt ggaccctgta
    11881 cctatccccc cgggaattcc tagagggatt cacttctttt cgcagatcag cgctgatctt
    11941 ccctgcggcc cttgtgcttc tgtctttctg cggttatcta atcgttcaga accggaggat
    12001 tcgccgcctc cgatggaaaa tcttcatcca caagaccaag atggagcggg ccatgggcag
    12061 catggaagag atgagggccc tattccagat tgcttcggcg actgccgacc ggaaggagct
    12121 tcccgaaatc ctggagatca tcgccaggga atctttgaac tgcttaaaag ctcatcgttc
    12181 cacggtcttc tccctggatg aaaagagcgg gattctcaag ccccacttca cctttacctc
    12241 cgaccccttg aatgaacaag tggggctatt tgaagaaaaa gagatggccc gcaagtccct
    12301 ccgccagaga aagggctttc ttctccggga gcctgcggat tttacggaat atctgaagta
    12361 ccttgagcga gaacggaaga tcacctcgct cctttgtatt cccctggttt cccaggacag
    12421 atcgctcggg gttctcagtg tagtcatgat cgatgacgac cggaagttta acgaaaggga
    12481 cctccagttt ctcatggtca tgggaattca agccgccatg gccgatgaaa aatcttcgct
    12541 ggatgcggag cttcgtaggg gggccagtta ccggaaagaa tacgagcagt acttagacca
    12601 catcctgaac cagttgcaga ccctctccga cgtggaaagg agacgcatcg aagatcacat
    12661 caaaaggctt ctccccacac ctgccggcga ggaaagggtc cccctcagcg aaccggccga
    12721 agaggcgaga aatggaagcc ctccggagac ggagccgatc gcccttgccc aagaggttga
    12781 agaccgggtc accaagatgc tgcaggtaga tatagaggga gaaccttcat ctcttgctcc
    12841 cgatctgggc aacggaggag ttttcatccg aactccgaac cccctagagc tgggggagga
    12901 gttcgtttta aaacttcata tggacgaagg ggaaacaccc gtcggagtaa cctgccgggt
    12961 catttggacg aacaaatacg ggaaggaaag ccgacacctc cggcggggaa tgggagtgaa
    13021 atttctggac ctctctcccc aactccggaa ccgggtggaa tcctacatcc aatctcacag
    13081 ggacaatcaa ttttctctgg ctgaagatca acaccatcta tcctttggag actaaataag
    13141 aaaaggggcg tcgccttatg ggaatctttt cctttcgtgc cgcaggagaa attacctttg
    13201 gagtcgggtc tgtaaatcga ctgggagccg ccgttcgcaa agcgggaggg aaaaaagtct
    13261 tggtggtggt agacccggga tttgccaaga ccggttcgtt caccagggtt acccaatccc
    13321 tggagcgaga aggcctgtct tttgttctct ttgaccaggt tgaacccgag cctcgcacgg
    13381 tcatcgccga ccagggtggg gaggtcgctc ggaaggagag atgcgacttc gtcctcgggg
    13441 tgggcggggg cagcgccatg gacacggcga aggcagcggc ggttctggcc accaacgggg
    13501 ggcaggcccg ggactatcag gggctggaca aggttccccg gccgggcctc ctgaaaggga
    13561 tggtccccac cacggcgggg accggaagcg aggtcacctt caccgcggtc ttcatcaatg
    13621 aagaggagaa aaaaaaggcg ggaatcaaca gcccccacct gtatccggag atgtccattc
    13681 tggaccccga attgactctg tcccttcccc cggcggtgac ggcctttacc gggatggacg
    13741 ctctggctca cgccgttgaa tcgtacactt ccaaggcggc ccatcccctt tccgagatgt
    13801 tcagcctgga gggcatccat ctcattggga agagtctgcg cagagcagtg gaaaaaggat
    13861 cgaacctgga ggcccgttcg gacatgctcc tggggagcct gctggccgga atcggcctgg
    13921 ccaacgccgg ggtgaccgct gttcattccc tctcctatcc cttgggagga cgcttccgag
    13981 tccctcacgg agtgggcaac gggctcctgc tccccgcggt catggaatac aacgccctat
    14041 cccttccgga gaaattcgcc aggatcgccg gggccatggg cgagaagacc gaggggttgt
    14101 cagccaagaa tgcggccctt ttggccgttg aatcggtaaa gggcctggcc cgggatattc
    14161 aagtgcccat gcgcctgagc gacctaggaa ttcccgtatc ggccttcccc gggatggcgg
    14221 aagaggccct gaaagtcacc cgccccctgg agaacaaccc caggccggtt tccttcgaag
    14281 acgccatcca gatctaccgc agcgtgttct aatttccatc ttgtccccgt ggcccgtttc
    14341 ggggtaagat tcgcttcaga tgaaccaaat gcggaatgcg gaattcataa aaagcgcaga
    14401 cttccgtcgc gtcttgcatt ccgcattcca tattccgaat tcttctaagg aggtcggcag
    14461 atgggtggag cagagttgat tggcagggaa gagcaggaag ccgtatctga ggtcatcggc
    14521 cggggcgttc ttttccgtta cggtttcaac gagaaaagaa agaacatctt caaggtcctg
    14581 gaatttgaaa accaattttc caaatacatg ggctgctcct atgccttggg ggtttcctcc
    14641 ggctcggcgg ccgtacgcgt ggcggtggcc gccctgggga tcaaaagggg cgatgaggtt
    14701 attacccaga gtttcacctt tgttgccacc gtggaaggag tcctggaagc gggggcaact
    14761 cccgtgctga ccgaagtgga cgcttccttg aacatggatc ccaaggacct ggaagccaag
    14821 atcaccgaca agacccgggc cattatgcct gttcacatgc tgggtgtccc cgcccggatg
    14881 gacgagatcc tggccatcgc ccgcaagaga aacatcccgg tgatcgagga ctcctgccag
    14941 gcctgcggtt cgggttacaa gggaaagaaa acagggaccc tcgggtccct ggggacctac
    15001 agcttcgact atgtaaagac tctgaccacc ggagagggag ggatggtggt gaccaacgat
    15061 aaaaaggttt acgagctggc ctgcgaatac cacgaccacg gccatgttca taacccggag
    15121 gtcccgcggg gagaggaccc gcaccgcatc accgggttca actaccggat gaccgagctc
    15181 cagggggcct tcggcctggt tcagttgaac cggctggatt cggtgctggc cgaccagcgg
    15241 aaaaacaagg gagagatcaa aaaggccatt gccgcgatcc cccaggtgaa attccgcgac
    15301 cttcccgatc cggcagggga cggaggggac accctggcct tttttctccc cgatgccaag
    15361 gtggccaagg cattcaatga ccatctggct aaatcaaagg tggataccaa aatcctcccc
    15421 tcggccatga attggcattt ttccggcagc tggaagcata tgattcaaca tttgcccccc
    15481 tatcgaacgg acgcttggcc aaaatcggag gctctgttga aacgagcgat tgccctgccc
    15541 atttccgtgc ggatgagcga tgagcagaag gcaagggtgg tcgaagccgt tcacaaggcg
    15601 gcgaaggaag tactttaaga cagttcgtag tttggagttc ggggtaagaa acgagcctgg
    15661 atgggtattg gcgccggact ccgaactcat tcctccgaag ggatcggggc ttttccgcgg
    15721 aatcagggat tgaaggattc aagatgaagg tttacgggat tattcctgca aggtatgggt
    15781 ccacccggtt gcctggaaag gtcctggccg acatcggcgg aaagcccatg atccagcatg
    15841 tgtatgaacg agcccgtcgg tccccttccc tggatcgtct ggctgtggcc acagacgacg
    15901 accgggttta tcagaaggtc ctcgattttg ggggggaagc ggttaagaca tctcctggcc
    15961 atccttctgg gaccgaccgg gccgctgaag cggcacgggt cttgggggcg caggagaaag
    16021 acctgatcgt caacatccaa ggggaccagc cgctctttga gcccgggatg gtggatgaga
    16081 tcgtgggtcc cttccggaag gatccggacc tcctcatggg agcattggtc tatcccatcc
    16141 gcagccggga agagctgctc aatccgagtg tggttaaagt agtggtagac aagcttggat
    16201 ttgctctata tttttcccgt tccgctatgc cctatgtcat agaatcttcc atgactccgc
    16261 gctattttaa gcatataggg ccttacgctt accgaaccgg ctttttgatc caatttacga
    16321 agatcgaacg gggggaactg gagcaggccg agtccctgga acagctgagg gctttggaaa
    16381 acgggtatcg gatcaagatc gtagtgactc aatatgactc ccaggaggtg gatactccgg
    16441 aagacttgga aagagtgagg ataaggatcg ccggggtaaa atagactctt cttcccgagt
    16501 gaccagagga gcggaaagaa gaggtctttt agaggaggga agaaagagaa attgagggaa
    16561 agttcttcct aaggggcggg ggcagatcac ttcgtgttta ttttttataa tcttctgctg
    16621 ttggtcttct cagtcggggc gtttcctata tttataacta agctggtttt ctcatcccaa
    16681 aagcgcaggg tctatgccca gaagctcggg ttccttccat ggagaacgct tgagaaagta
    16741 aaaggccaac cccgggtttg gattcacgcg gtttccctgg gggaagtcgg ggccgttcat
    16801 ccgctgatac gggaacttag gagggtttat ccggacgcct gtttgatgct ttccaccggg
    16861 actgagagcg ggcagaaaat ggcccgcgag cgcctgaccg aagcgaatgg aactttcttc
    16921 ttcccctggg attatccctg ggtggtgcgt aaggtgattc gccgattgag accggacctc
    16981 ttcgtaattt cggaaacaga actctggcct aacttcctca agatcgccaa agaggaaggg
    17041 gcgaaaactc tactggtgaa cggtcggatc tccgaccggt ctttccgaag ataccgcaag
    17101 gcccgatttt tctttaccgc ggttctggac aactttgacg ccatgtccat gatccgggtc
    17161 caggatggag aacgcataat cagcatgggg gccaatccgg tccatgtctc ggttaatgga
    17221 aactgcaagt tcgaccaggc cgcctcctgg gcccttcccg gcgttcagga cgaggttcgg
    17281 agaatcttgg gagtcaatcc acaggatcgg gtcctggtcg ccggaagcac ccacgaagga
    17341 gaggaagagg ccgtcataaa ggccttcctc aggctccggc agagcgatcc ggagaccctg
    17401 ctgatcctgg tcccccgaca catcgatcgt tcggatcggg tggccagact tctggaacgg
    17461 catggaatcc gagaatgggt ccgacgcagc cagctgaacg gggaggggcg aaagggggcc
    17521 aaggtggtcc tgtgggatac ctttggggag ctcttcaaag tttatagctt ggcgaccctg
    17581 gtcttttgcg ggggaagcct ggttcccaag cggggtcaaa acattctgga gcccgcagcc
    17641 tggggcaagg ttgtccttta cggcccatca atggaggatt ttctcgacgc tcatcagttc
    17701 ctgtccagcg ttggggccgg aatcatggtc caggatgagg aggagctcac ggatcgctgc
    17761 ctgtattttt ggggtcaccc ccgggagctg aaagagaggg gagaagcggg gaaagaggcc
    17821 ctttgggctc agcggggtgc cacccaacga aatgtagagc tggttagaag gctgctggga
    17881 agatgaatcg gaaaaaagcc gttctggccc tggaagacgg aaaagttttc gaaggctggt
    17941 ccttcggtgc tccgggagaa cacgcgggag aagtggtttt caacacttcc atgaccgggt
    18001 accaggaggt cctcaccgac ccttcttaca aggggcagat tgtgaccatg acctaccccc
    18061 tcatcgggaa ttacggggtg aatgaggagg acctggaatc cacccatcct catgtggaag
    18121 gtttcgtgat caaagagaac tgccccttcc ccagcaattg gcggtcccaa ggaagcctga
    18181 gtgattattt ggaccgtttt gggattgtgg gaatcgaagg agtggatacc agggcgctga
    18241 ccaaacatct gcgcacggcg ggagccatgc ggggaatcat ctccacggag gacctggaac
    18301 cctgttcctt gattgataag gcgcggacct ctcctcctat ggtagggagc gactgggtca
    18361 aggcggtgac ctgcgaggct ccctatgaat ggggccgcgg ggaagaggga aagaaaaggg
    18421 ttagggggac agggctgggt ctatgggaat ggggaagggg gcaggccatc cttccctcca
    18481 caggaaagaa gtaccgggtt gtggctatgg atttcgggat gaaacacaat atcatccgcc
    18541 atctggccag ccgggggact caggttctgg tcgtcccggc caagacccag gcggaggaaa
    18601 tcctggacct ggaccctcat gggattttcc tttccaacgg tccgggggac ccggcggccc
    18661 tgcccgcgat tattgacact gtccgtcaac tcctgggaaa gaggccgatc ttcggcatct
    18721 gtttgggcca ccagcttttg ggcctggcca tggggggaaa gacctataaa ttaaaattcg
    18781 ggcaccgggg agccaatcag ccggtgaaaa atcttatcaa tgggaaagtg gaaattaccg
    18841 tccagaacca tggtttttgc gtggacctgg acagcctgcc cccgggccag gtgcagatta
    18901 cccatatcaa tttgaacgat caaagcctgg aagggatgcg ccatcgctgg cttcccatct
    18961 tttcggtaca gtaccacccg gaagcttcgg ccgggcccca cgatgccgcc tatctctttg
    19021 atgagttcat atctttgatg gacagacact cgaaagaatc atgcccaaac gaaccgacat
    19081 ccaaaaaatc ctgatcatcg ggtccggtcc gatcattatc ggccaggcct gcgagttcga
    19141 ctattcgggc acccaggcct gcaaagccct gagagaggaa ggattcgagg tcgtcttggt
    19201 gaattccaac ccggccacca tcatgaccga cccgcaaatg gcggaccgga cctacatgga
    19261 accggtgacc cccgaggtag tggccaagat tgtggaacgg gagcgccccc aggcgctcct
    19321 ccccaccctg ggggggcaga ccggtttgaa cacggccatc caggtggacg agatgggtat
    19381 cctggacagg ttcggggtag agatgattgg agccaatcag gaagccattc gcaaggccga
    19441 ggaccgggag ctcttcaagg aagccatgcg ccgcatcggg ttggaggtgc cgcgaagcgg
    19501 ttttgcccac aacctggggg aagccaggga tgtggttcga gaaattgggc tgccggtggt
    19561 cattcgtccg agcttcaccc tgggaggaac gggcgggggg atcgctttca acctggagga
    19621 gttcgaacgc ctggccctgc agggaatcga gctcagcatg atccacgaga tcatgatcga
    19681 ggaatcggtg atcgggtgga aggaatacga gctcgaggtt atgagggacc ttaaggataa
    19741 cgtggtcatc atctgttcca tcgagaactt tgaccccatg ggggtgcaca ccggggacag
    19801 catcaccgtg gccccggccc aaaccctgag cgacaaagag taccagcgca tgcgcgacgc
    19861 ggccatcgcc attatccggg agatcggagt ggaaaccggg ggctccaaca tccagtttgc
    19921 cctccatccc cgggacggcc gcatggtggt catcgagatg aacccccgcg tttctcggag
    19981 ctcagcgctg gcctccaagg ccaccggttt ccccattgcc aaggtggcgg ccaaactggc
    20041 ggtcggctat accctcgatg agattcccaa cgacatcacc cgcgagacca tggcttcctt
    20101 cgaaccttcc atcgattact gcgtggtcaa gttcccccgg tgggctttcg agaaattccg
    20161 gggggccgac cccactttga ccacctccat gaagtcggtg ggagaagcca tggctgtggg
    20221 acgaaccttc aaggaggcgc tgcaaaaggc catccgttcc ctggaaatcg gccgctttgg
    20281 cctgggaatg gacggcaaag gggaacccga tcctgaccgg atgaaggaaa aactggccca
    20341 tcctacccca gaaagggtct tttggctgcg cgccgcgctc aagagtggaa tctcacctgc
    20401 ggagatcgca gaaacaaccc ggatcgacca gtggttcctg gagaatatga aacaggtctg
    20461 cgacctggag gaggagattc agaaaatccg cctcgacggc cgagggctgg agagtctccc
    20521 aacttacctc cttcggcagg ccaaagagtg gggatactcg gatgtccaga tcgcccacct
    20581 cctgggttca gatgagatgg aggtgcgtaa gcggcgcaag gccttgggaa tctgccccac
    20641 cttcaagctg gtggacactt gcgccgccga gttcgaagcc tataccccct attactattc
    20701 taccaatgaa tcggaagatg aagttatccc ttccgacaag aagaaagtca tgatcctcgg
    20761 gggaggcccc aaccggatcg gccaggggat cgagtttgac tactgctgcg ttcatgcctc
    20821 cctcgctctg cgcgaagagg gattcgaaac catcatggta aacagcaatc ccgagacggt
    20881 ctccaccgat tatgacacct ctgaccgcct ctattttgag cccctcaccc gagaggatgt
    20941 gctgaacatc gtggagaagg aaaaacccaa gggagtcatc gttcaattcg gcgggcagac
    21001 tcccttaaac ctggcagtgc ctctggagaa agcgggagta aagattctag gtaccacccc
    21061 agatagtatc gaccgggccg aggaccggga gcgtttcaag gagctcattc agaaattagg
    21121 gttggtccag ccccaaaatg ggaccgccac ctccttcgaa gaagcccggg ccgtggcccg
    21181 ggaaatcggg taccccgtgg tggttcgacc ctcctatgtc ttaggcgggc gggccatgga
    21241 gatcgtctac gacgaggaga gcctggagcg gtatatgcgg gaggcggtga tggcctcccc
    21301 ggagcgcccc atcctgatcg acaagttcct ggaggacgct atcgaagtgg atgtggatgc
    21361 catctccgac gggaaccggg tggttatcgg cggaatcatg gagcatatcg aggaggcggg
    21421 gattcattcc ggagacagcg cctgcgttct tcctccctat accctgagcg atgatttaat
    21481 ggaagtgatt aagcagaata cgtacgcttt ggccaaggag cttcgagtcg aggggttgat
    21541 gaacattcag tatgccattc gcgatgaccg ggtttacatc ctggaggtca acccccgggc
    21601 ctcccggacg atcccctttg tctctaaggc gaccggggtg ccgctggcca aactggcggc
    21661 caaagtgatg atcggccgga ccctggaaga gctgggcttt acccaggagg tcgaggtatc
    21721 ccatgtgtcg gtgaaggagg tggtctttcc cttcaaccgc ttcccgggag tggacaccgt
    21781 gctgggcccg gagatgaagt ccaccggcga ggtcatggga atcgatcatg ctttcggcat
    21841 tgccttcgcc aaggctgagc tggcggctgg gcaggtcctc ccgcgaacgg gaaaagtatt
    21901 tatcagcgtg aagaaccggg acaagcgggc catcatcttc attgccaaga agctttctga
    21961 ttttggcttt cggatcgtgg ccacccgggg gacggccaag gcgctctcgc agaacggaat
    22021 cccggtggag gtaatccata aggtccacga aggaaggccg aatgtcgtgg atgccatcaa
    22081 gaaccgggat atcaacctga tcatcaacac ccccaccggc cgacatccca agaaagatca
    22141 ggtttccatc cgggctgtag ccgtggctca taatatccct ttgatcacca ccatctccgg
    22201 ggcagcggca tcggttcatg gcatcgaatc tctgctccag gaagaagtcc gagtgaagtc
    22261 cttgcaggag taccatcaaa aggaaagtcc aagagtcgcg gccgccaaag gcgcacagac
    22321 ccaaccaacc acaggggatt gaaacccatg aaaacgatag attgtccaaa agaagaatgc
    22381 ggaattttcg gaatcttcga tcatcccgag gcctctcact tgacctacct gggactctac
    22441 tcgctgcagc accggggaca ggaaagcgct ggcatcgtga ccggctatga gggggagccc
    22501 aagacccaca aggggatggg gctggtggcc gatgttttcg atcaaaacac cttgaaagct
    22561 ctctgcggaa attccgccat cggtcatgtc cgctactcta ccacggggtc ctccctcctg
    22621 aagaatgccc agcctttcct ggcccagcgg cagaagagtt tcatggctgt ggcccacaac
    22681 ggcaacctgg tgaatgcccg gcaattgaaa gaccgcctgg aggcccaggg atcaatcttt
    22741 cagtcgacca tggacagcga aatcttcgtc cacctgattg ccaggtccag gagaaaggga
    22801 tatcaggaga ggaccattca ggccctctcc caggttcagg gggctttttc ccttatcctc
    22861 ctcacggaga acggattgat cggcgcccgt gatccccatg gctttcggcc cctttgcctg
    22921 ggaaaactgg atggaagttt cgttctcgcc tcggagacct gcgccctgga cctcgtgcaa
    22981 gccaagtaca tcagcgacat ccaccccggt gaagtggtga tgatcgataa gcacggtctc
    23041 cactcggaga aaccatttcc cagggtgaag cctagtttct gcatcttcga gtacatctat
    23101 tttgcccggc cggacagcaa catctttggg gagaatgtgt atctggtgcg caagaaactg
    23161 ggggctcgtt tggcggaaga agctccggca gaggccgatt tcgtcatgcc cattcctgac
    23221 tcgggaaact acgctgccct tgggtttgcg gagaggttga gacttccttt tgagatgggg
    23281 atgatccgca atcactatgt ggggcggacc tttattcagc cctcgcaaag cgttcgcgat
    23341 ttgggggtaa aggtcaaact caatccggtt aaggacctgc tccggggcaa gcgggtggtg
    23401 gtcgtcgagg attcgatcgt ccgcgggacc accagccgta caagaattcg ggctttgcgg
    23461 gatgccgggg tcaaagaggt ccacatgagg gtcagctgcc ctccccatcg cttttcctgc
    23521 cattacggaa ttgactttcc cactcgagaa gagctcatcg gttcttctcg gtcggtggag
    23581 gagattcgca aatttatcgg gcttgacagt ctagcctact tgagtgtgga gggaatggtg
    23641 ggagccatgc cgcaatccag ggacagcttt tgcctggcct gtttcactgg gaaataccct
    23701 gtccccttct ctccacggac gaccaaattg gccctcgagc aataaggaaa gctgcggaat
    23761 caattcaaaa ttccaccctt tttccccagg acccgaaaaa ggaaaaccgc tctttcagct
    23821 ctattatccc tccttttcct gccttgaaaa ggttttgatt ttgtgaattc aaaaattcat
    23881 ttaaaaatca attggttgtt taaaataaat tctttccaag cccaattcta aaaaaaagaa
    23941 aaaaaaactt gacaggcagg ggggtctgtg ctaaaaatcc gttgaatgaa taatcattca
    24001 gtggcttttt ttgttttccc catttgggca taaccgaggg ggctgcaaga acccaaggta
    24061 tataagtgct ggcaaaaatt caactagctt ttatgaaaga cgaaagcaaa caccagaaga
    24121 tccttcaagc ggcagttaag gttttttcgg aaaaaggatt ctacaactcc cgagtttccg
    24181 aaatcgccaa agaagcaaat gtggctgatg ggaccatcta cctctatttc aaaaataagg
    24241 acgacatcct tatctcgcta tttgaagaag aattcggaaa gattgtccag aacatgcggg
    24301 ccgcgctgga aaaggacaag gatgctctgc agaagatcaa gcgctttgcc atcacccact
    24361 tatccatcgt tacgaaacag caggaactcg ccgaagttat gggtgtagag gtccgccaga
    24421 gcagtaagtt catgaaagag tacgtcaaca agcccttcat cgaatacctg aatatcattc
    24481 gctcggtggt gatcgaaggg caggaaaagg gcctcatccg gaaggacctc actccgggga
    24541 tcatgaaaag agcctttttt ggagcactgg atgagatggc gcgctactgg gtcctatcga
    24601 ctcaaaaaaa acacagcacc gacgatgctg cattgcagat cagcgacgtg ttcatccggg
    24661 ggatgatgag cgaagaagcc cggagaaact acacctggcc ttcttgattt tttgattggg
    24721 acgcagaaga acgcggatca aaaagggatt gattccataa agcgttaacc ttggttttcg
    24781 ggttccaaag gccaggatat tcgtttccag ccattttttt cgggcatcga tcgggggccg
    24841 tgatttcaag ttcgggagca aaagaattcg gccattttga aaaactttgg ttacattcgt
    24901 ttccttctgc gttccattaa ccacgttcga gaaaaggagg ttcaaggtga atgtcattgt
    24961 ttgcatgaaa caggtgccgg ataccgaggc tctgatcaag gtgaaacccg atgggtccgg
    25021 gattgtgacc gatgatatca agtacgtcat gaatccctat tgtgaattcg ccgtcgaaga
    25081 atccttgcga atcaaggaaa aacaaggggg cgcaagcatc ctggtcactc tgggaccggc
    25141 gagggcggtg gaagccctgc gcacggggct ggccatgggt tccgaccgct cagtccacct
    25201 caatgatccg gctttcgaag ggggagatgg gtttgccacc gctaaggtct tggctgaagc
    25261 catcaaaaaa gagccgtacg atgtaatcct gtgcggcaaa caggcggtcg acgacgatat
    25321 ggcccaggtg ggacagtctt tggccgagct gctgaatctc ccgcatatca ccctgatcac
    25381 caaactggag ttcagcgcgg ataagaaaaa agccaaggtc gaacgtgagg tagacggggg
    25441 caaggaggtc atcgaggtta acttaccggc cgtcttcacc tgccagaagg gcttaaacga
    25501 gcctcgctac gcctcccttc cgggcatcat gaaggccaag aagaaggaag tcaaggccat
    25561 caacctggcg gctttgggga tccctgcgga tcagaccgga gcggcaggct ctaagatcaa
    25621 agtcctaaag tacagctctc ctccttcccg cccgcccgga aaaatcgtca gtggggaatt
    25681 gcttgaagca gctcagaagc tggtcaaact tctccgggag gaagcgaagg tcatttgaga
    25741 ccttgccggg agggacagat tcagggctag gataaatcat tcattctatt cgggatagcg
    25801 taaaaaggag atgatccatg gctaaaggaa tttgggtatt ttcggaagtc aaggataaca
    25861 acatccgcaa ggtcactttc gagcttctca gccagggaag aaagatggcc gggaaactgg
    25921 gagaagaggt cgtagccgta cttctgggat cgggggtgga ggggctgacc ggccgcctgg
    25981 ccgaatttgc cgacaaggtc ttttgggtcg atgctccagc tctggctcag tacactacgg
    26041 atgcttattc atcggttctg acccgtctct taaaggagca tcagccctcc atctttttat
    26101 gcggggccac ggtcatcggg aaagaccttt ctccgcgatt ggccacccgt cttcaaaccg
    26161 gattagcgac agactgcatc agcctctcca tcggggatga cggagcgatg gtcgccaagc
    26221 ggccggttta cgccgggaaa gcttacatcg aagtgaccta cccggtggcc aggcctcaaa
    26281 tggcatccgt gcgtcccaat gtgctggaga tggtgcagcc cgatggaacg aaaaaaggat
    26341 cgatcatcaa ggtcgaagcc cagatcgatg ccggcagcct tcggacgacc gtgttggagg
    26401 tggtcaaatc cgccgcggcc aaggtggatt tgactgaagc ggagatcatc gtatcggggg
    26461 gccggggaat gaaggggtcg gagaacttca aaatcctgga agaactggct gaggtcatca
    26521 aggctaccgt gggggcctcc cgcgcggcag tggactccgg ctggcgcgaa cacggcgacc
    26581 aagtggggca gaccggaaag gtggtgaccc cgaatctata catcgcctgc ggtatctccg
    26641 gcgccatcca gcacctggcc gggatggggt cttccaaagt catcgtagct atcaacaaag
    26701 acccggacgc gccgattttc cagaaggcgg attacgggat cgtggaagac ctcttcaaag
    26761 cagtccccgt gctgaccgaa gagttcaaaa aacttctttc ggagagctga tttcccaagg
    26821 ggggactctc cttccgcggg agccccccat ttttttgctc cgcccaattt ctttcccatc
    26881 gtctcttcaa gtgaaggaag gcggactctg gaccgctagc cctagattca gccggtggcc
    26941 tcaccggttg tggggaatcg aggacctata tgctcagctc attcgacctt ttcctgctat
    27001 tcatcgtggc ggtggtattt gcttatggcc tgtatcgaag gtaccgccta tggatgatag
    27061 gcaaacctga aaagagaatc gatcgtccca aggagcgact gatctctttg tgggcttatg
    27121 tggcgggaca caaacgcatg cttcgggacg tttatccggg ctggatgcat ctgctcatct
    27181 tctacggttt tctggttcct ttcgtggtgg tggccattac ccaggcgaac ttctccctgc
    27241 ccagagctct gtcccttgcc ctatccttgt tcttcgatct ggtggggacc ctgggaatcg
    27301 ttgggctgct catggccgct taccgccggt atgtgcagaa gccggataac ctgacctatg
    27361 atacctactg gggcaacgcg gtcgccctca tctttctcct ggccattttc ggcctgggtt
    27421 tctgcgtaga gggtttgcgg atcgcccgga cccaacccga ttgggtcgcc tggtcgccgg
    27481 ttgggtggct cttctcccaa tttttcagcg gactaaccga catccaccag gtccttttgc
    27541 atcggattct ctggcgcctt catctattct tggtcctcgg tttcatcgct ttcattcctt
    27601 attggcgcat gctgcacatc gtaaccggac cggccaatgt ttacatgcgt tccctgaccc
    27661 cgaaggggga attgcctccg atccgggatt ttgagaccgc cgaaaccttc ggggcatcca
    27721 aaatttccga attgacccgc aagcagctct ttgacttgga tgcctgcacc cggtgcggcc
    27781 gctgcctgga ccactgcccg gggtcggtca gtggcaaacc cctggctcct aagaagaatt
    27841 gggataccct gagagcccac ctggaggaaa aggcggccct gatgcggaag ggagtcgacc
    27901 cgtatgcgga agggcagacg aaactgatcg gcagtgggac aggggacggg gtttcggagg
    27961 atgtgatctg ggcgtgcacc aactgcctgg cctgcgccat ggtgtgtccg gttttcatcc
    28021 cctgcgtgga taaatttttg gaaatgcggc ggtatctggt cctcatggaa agcaacttcc
    28081 cccaggaagc gcagctggtc ttccgcaaca tggagaacaa cagcaatcct tggggcgtag
    28141 ggtctggact tcgggccgat tggatgaaag gcctggaagt caagaccatg gctgaaaacg
    28201 gcgacattga ccttctcttc ttcgtcggtt gtgcgggcag cttcgatgat cgaaataaaa
    28261 aggtggctac ttctttggtg aaaatcctga acgcctgcgg ggtgaagttc tccgtcctgg
    28321 gcaccgaaga gggatgctgc ggggattccg cccggcgcat cgggaacgaa tacctctacc
    28381 aaaccctggt ccagaccaac atcgaagtgt tcaagaatta caacatcagg aagatcatga
    28441 ccatgtgccc ccattgcttc aacaccctca agaacgagta tcctcagttc ggggggaatt
    28501 acgaagtgat ccattacacc catttcctag cggaggcgtt ggggagcggg cgcctgaagc
    28561 tgaagaaatc tttggaaaaa gtgatcacct accatgactc gtgctacctg ggtcgggcca
    28621 atgaagtcta tgaggagcct cggcgcatca ttcaggcgat tcctgggctc aaattggtgg
    28681 aaatggagcg gaatcatatc cgcagtttct gctgcggggc cgggggcggc cggatgtgga
    28741 tggaggagaa gctgggaact cggattaacc agctccggac cgatcaggcg cttcagacca
    28801 aagccaattg ggtggggacc gcatgccctt actgcctgac catgctcgga gacggaatca
    28861 aggagaaagg cctggaagag tccatggccg cccatgacct gagcgagctc gtggtacaag
    28921 ccatggattc atgaagcccc ccgacaaaag gagagccgaa agcggcaaga taggagtttc
    28981 tttccttcac atttacccgt ccctggaccg aaaaagagga aaaggtaatg gtcagaaacg
    29041 ataagcgctg cattggggaa tgcattcgaa ccctatttcc atttttggct ttcttcttat
    29101 tcttggcttg tgccgcgcat gcggcggagg acgccgaatg cctgaaatgt cataagaatc
    29161 cccgcctcag caaggggaac aaagatgggt ctctcctttc cttgtatgta aaggaggagg
    29221 cctttaaagc ttccgtacac ggagcggctg gcatggggtg cacagactgc catcaggagg
    29281 ccaaaccgaa cgtccatccg gcggaaggct ttcctgaagt cgggtgtgcg aactgccacc
    29341 aggactcggc ggaggcctat aagcagaccg cccatggaat gatgctcgaa agcgggatgg
    29401 aaagggcccc caaatgccag gattgccata cttcccatta cacgcgaaaa attgccgacc
    29461 cgcaatcacc ggtaaaggct tcccgccttc cccaagcctg cgccaagtgt catgaggcgg
    29521 cccaggtgcc gaaagggttc ctaatggcgc tcgctactta ccgaatcatg gggcatccca
    29581 aaaccagttt ggaatacagc tatgataccc aaggttgcgc caactgccat ccggacaatg
    29641 caggacatgc gcagaagaag ggccctcctc cctcctgtgt caaatgtcat gacccctcct
    29701 tttccaaacc gctcctcctg gggccgatcc atttcaaagt atccttgggg gaccaaccgg
    29761 ttcctttcct cctgcgcttc ctttacgggg ccggcctgat cctcgtcgtg ttggcctgca
    29821 ttgccttctt cggctatcgc acctataccc ggagaaaggc acggaagccg gcgggggaga
    29881 agcccaccga agggcaagga acgacttcct aaaaaggaac gattccgaaa atcggcccga
    29941 gaggccggga atctcgatct gctttcggga agaggcaagg agaaagggac gggcgatttc
    30001 gtttctgctt gccttttttt cagggttctt gccaggcccc aggtaaagtg atataaaagt
    30061 cattgctcta tttttctcga atccaaaaac cagatgcagg accatatctt ttaaggaggt
    30121 tccgaaatgg attttcagct ttcaccccag caggagctgc tcagaaaaag cgtgagagaa
    30181 ttcgccgaag gggccgtcgc tcccaaggtc acgcacatgg aggagacgga tgaggttccc
    30241 tgggaccttt acaaggagat gtccaaacag ctgtacatgg gcgtgctcat ccccaaggag
    30301 ttcggcggga ccgccttcgg ccatttggcc cggatgatca tgctcgagga ggtcgggcgc
    30361 gtttcggcgg ccacggccat ggctttgcag atcttccacc tggggacggt gcccattgtg
    30421 gatttcggca atgaagccca gaagaaaaaa tacctcccag gcatggccaa aggggaacgt
    30481 ctgtccacca tcgccgtaac cgaagccact ggcggttccg atccgaccgg aatccagacg
    30541 acagctaagc tgcagggtga cagctacatt ttcaatggaa ggaagtgctt catcacccat
    30601 ggtcatgtag ctgatgtcct gaccatcatg gccaagaccg gcgaagggcc gaaggggttc
    30661 agcgctttca tcgtggagaa gtcctttccc gggttcaagc tcggccggaa ggaaaagaaa
    30721 ttcgggctcc acgggtgcaa cacgggcgag attgccatgg agaactgcgc cgtgccccga
    30781 gggaatcttc tggggagcga aggagacggc cttaaagtct ccatggccgc catcagcgaa
    30841 gtgggacggg ccgggatggc aggatgtggc ctgggggtca tcaacgcctg cctggaagcc
    30901 tcggtgaagt tcgccaacga aagaattctg gggggtaagc ccatcagcca gcatcaggcc
    30961 attcagtgga tgatttcgga catctacatg gacctggaag ccagccgcct gctggcttac
    31021 cgggcctcct ggctcaagga tcagaaagtc cggtgcgata cggacatggc catggccaag
    31081 tactacgcca cggaggccgc ggtgcgctgc gccaagaagg ccgtggacat ccatggcggg
    31141 tacggataca tgatggaata cccggtgcag cggtactacc gggacgcgga gatcctcatc
    31201 gcttcggcag gtacctcgga gatccagcgc atcgtcatgg cccgcaaagc cctgacgtct
    31261 tttaaataaa ttttttcacc accgagggat agagaacgca gaggttgata aataacgaaa
    31321 ttcaaggtcc aaaatcccga aatcataatt caaaagttag cttaagagtt tatccttatt
    31381 tgggatttgg tgtttgaggc ttgttatttt ccgctgtgtc gcggtggtga aaggtttttg
    31441 ggtatgaata ttgttgtctg cgtcaagccg gctccggacc cccagcgctg ggacagcatc
    31501 cagctggacc ccaagactaa ggccttgcac cgggatggga ttcccagcgt cctggggccc
    31561 ctggacaggc gggccctgga agagggcctg cggatcaggg agaagctcgg ggggaaggtg
    31621 gttgctatgg ccatggcccc tccctccgct cgggacaacc tggtggaagc cctggccatg
    31681 ggggcggacg aggctgtcat cctgagcgac cgggcgtttg ccgggtccga cacctgggcc
    31741 acttccttcg tcctagccag gggcgttcag agatcgggtg cctttgatct ggtcctttgc
    31801 ggggaatgga gtctggacgg gtccaccggc cacgtgggaa cccagctggc cgagtatctg
    31861 gggattccca atgcgagccg ggtcgcggag gtggagagcc tggaaggcgg gaccctgcgg
    31921 actcgaacca tgatcgaagg ggggtaccgg attctggaga cgcccctccc ggccttgatc
    31981 accgtgaccc gcgacatcaa tacccctcgc ttcacgtctc tgctgggggt gatggaggcg
    32041 gaaatgaagc ccctggtcat ctggtccgca gctgaactgg gaatccgacc ggaggaggtc
    32101 ggattcagcg ggtcccccac ccaaaccggc gatgtcttca tgccggagat gaaacggaaa
    32161 ggggaggtgc ttcaggggga gcctgaggag atggtcaaag agatcatccg gaaggtcaga
    32221 caagcgctgg ggtaaaagga aagaggacga ggagcgaggt acgaaggcat aaagaactcc
    32281 aagccttttc ttgggacctc ggtcctcgcg cttggacctg tcttcttaac gatcacaccc
    32341 aacaatcaaa atggaccaga gtcaattgaa agagtggaaa ggggtctggg ttttcgtcca
    32401 acaggaagaa ggctgtgtgg ctgaggtctc ctttgagctt ttgactgccg ccagagaact
    32461 gtcctccaag agggggaacc ccgaagttgt ggccctcctt ctggggtacg aagttaaggg
    32521 gctggctaaa gacctccttt cctatggggc ggatcgagtc ctctgcgcgg atcatccctc
    32581 actgggaaaa tatttaaccc ttccctacac ccgggttatc ggccgtctga tctccgagag
    32641 aaaacccgat atctttttgc ttccagccac ttcggtgggg gccgacctgg ccggtcgggt
    32701 ggccatccgg gtgaatactg ggctctctgc ccattgcacc cacttggata tcaatgccgg
    32761 gggagaactc atgcaagttg ttcctgcttt cggcggaaag gtgatggcca ccatcctctg
    32821 tccgaagcat cgccctcaga tggccactat ccgcccgggt gtttaccgaa gaggagagct
    32881 tcgaaaggga cgaggagagg tggagcaggt ctcccccgac attcaaccgg aagacctagg
    32941 gcagagagtg gtcgaaaccc attgggagaa gaagcctgcg caggccgtcg aggaagccga
    33001 ggtagtggta gccggcggag cggggatcgg gaatgccgaa gactggaaat gggtggaaag
    33061 gctggcccag gcccttcatg ccgcagtggg ggggacgcgc cctcccctgg atgaaggatg
    33121 gatcaaagaa ggccagatga ttggccagag cggaaagacc attcgaccca agctatacat
    33181 cggagtcggc atctccggag tcatccagca cgtggtgggg atccaggact ccaaagtgat
    33241 catcgccatc aacaacgatc cccatgcggc catcttccaa tccgcggacc tgggggcagt
    33301 gacggatttc cgcaggatcg ttcccctcct agtccaagaa ctgcagaaaa aaaacgagcc
    33361 gctttaccgg tgagggcccg tcccttcctt tttgcaggga aagggctttt cttctttaga
    33421 tggagaaact tgattgggga gggagatgga tgaaaatcga tccagcaaat ttagaccgaa
    33481 aaagtgcgca tgaaataatg gtagggtcgg cattgccgag gccgatcgct tttgtttcca
    33541 ccatcggccc ggacggggtt tataacctag cccctttcag tttcttcatc cccatgtcgg
    33601 tgaagccagc catcgtgggc gtcgggattg gccggaaggg agacggaaaa aagaaggata
    33661 ccctggtcaa tattgaattt tccaaggaat ttgtcataaa tctggttacg gaagaaatag
    33721 ccgaagccat gaatcaggct tcgggagagt acccgagcca tgtggacgag tttaaggagg
    33781 caggcctgac gccggttaaa gccgatttgc tcaaatcccc cagggtggcc gagtctcccg
    33841 taaatatgga atgccgcctg ctgcaggccc tggagtttgg agaatttccg aggattaata
    33901 cctttgtgat cggggaagtg atccgcatcc acgtgaaaga tgaattttgg agaaatggcg
    33961 aaattctatc ctcaaagctg aaggctattg gtcgtatggg gggagacctc tattgccgga
    34021 caagagaggt attcgagatg aaaaggccag tcatcgaaga gaggtggtga ggaagtctct
    34081 ccttttctgg agagagacat gaattttttc ggaatgtagg aagatccccc tcccctacaa
    34141 aaagggggag ggggcaaagg gatttactta acccttgctt ccgcagcctt ccactccaca
    34201 ttcttgaaaa atgaggcgat gtaatcgggt ctcttcaagc catagtcaat catgaaggca
    34261 tgttcgaaga catcgagaat caggatggga ttgcagccag ccaggtggcc tacatcgtgc
    34321 tcattgatcc agcagttgca tagagccccg gagacgctat cctggtagag gatggcccag
    34381 ccgattcccc gcatggttcc cactcctctg aagtcttttt cccactcctc gtaatttcca
    34441 aaagattcgg cgattttctt aaagagcttt ccgcctttgt cgatcgcccc cttgccgccg
    34501 aggttttcga agtagtactc atgaagcctc atcccgttga actcccaacc gaagcgtctt
    34561 ttcaactcgg cgaattcggg ggtcgcggtc ttaccctctt tcagcatctg tcccagggtg
    34621 tccatcagtt tattggtgtt ggtcacgtac ccctggtaga gggtgaagtg gttcttcaaa
    34681 agggtctcgc taaatccctc catcccgatg agttttccgt aatctttggc tgcgtaaggc
    34741 attctgttct cctttctcgc ctgagcgaaa gtaatgttgc ttcttccagt taagaagtaa
    34801 aaccctccca ttgcggaaag ggccaaaaac tggcgtcgga gtaaggatac aggtctcata
    34861 tcggggcagg actccttccc ctgattctga aatcaaaaat actagatcag cgagggaatg
    34921 agaatagggt gaatcctgta tttttggtcg ggaaacaagg tattttatgg ggggaaggag
    34981 tccctccctg cacgttgaat catttcttct gaaaagagat tccttccgat ttggtaagct
    35041 ttccctggta gacaaaagag acctggcgca gcgccgcttg gaaatcgaaa gggttcaaag
    35101 cggaaatggt gtcgatgggg acgatgacct gataccctcg cagagtggca gagccggcgg
    35161 catgaaggac gcagatgttg gccacggttc cggtgatcac caggttcttg atccccttct
    35221 ggcgcaggag caaatccagg tcggtggaaa aaaaaggatc ataggttctt ttccggacca
    35281 agaaatcttt cggggcaggc gataattccg ggatgattcg gccgcccgtg gtttcctgga
    35341 tggcatgagg gccccagatg gaaaattccg gatcatccgg gcgatgccag tcttgaatgt
    35401 agacgatcat aactcgagac ttacgggctt tccccaggag gctgcggatg ggagcgatcg
    35461 ttttccgggt gtcgggaacg aagagctttc ccttggggct gacgaaatca accagcatgt
    35521 cggaaaggag cagagcagtc ttcttggccg gaagggggag cctctcatag accctggaat
    35581 caggaacgga gacttgcata tttccctcct cttgagatag accaatcagg tcacaacggg
    35641 gcaggcaccg actaagagac aggttttacc ctgagcaccc tcctcccgga aatctggccc
    35701 atcctttttg caggaggctg ggagggattt cagagatgag cctttcgtct catctcactc
    35761 cagcaaaaac ttagttcctg gtatgtgtcc ccatttcgaa tagggcgctt gaaattttga
    35821 attttttccc tatttcttct gcggccgtgt cagcgtttgt aggatttctg gattcgcttg
    35881 ggaatttcgt actcacaggg aactttggtc tggcacagac cgcaaccggt gatctttact
    35941 ccatattcgc cccgcttgag aggcccgatg gtgttgtagc tgtattcctg gcatttgtct
    36001 ttgtcatggc ccctggaggt aatggccccg gcagggcacc gcagggcaca gaccttacaa
    36061 gtccggttga aaaaatagag gcagttggcc cggtgatggg ggtacttctt ggctgaaggc
    36121 ttcaagggca gattggtaac cacactaccg atccggatgg cttttcctct ggtggtgatg
    36181 aacccgtcgc tcaatccaaa actccccagg ccacaggcgt aggccgtatg cctttcggac
    36241 caactggaag ccagtcctat ccggggagac cttacccttc tccagtgagg ggaaatcatg
    36301 ggtgctacgg cctgataccc tttcttcttc agaaagctga cgacgtgttt tcgcaatttg
    36361 acattgaact gctccccaaa atcacgggcg tgggcccaca gccgggaggg gtatttggtt
    36421 tccttgcggt tgcttagccg aatctcctcg acgtggggca aaatccaagc gatgatagac
    36481 aatggctggg ggggcttctt gttcccgaag gtcaggcgga aaatctcctg gggggaaaag
    36541 tgaaattggc caatgatttt tttataagcc ttaaatagag ggtcctcccc cgaagcgaat
    36601 cccaccaggg gccggcccca gtatcttccc cggccttcct ttctccggtt tgccgggctc
    36661 gtctggatga aatggacaat ggccttttcg atgaacttct ctgggtcctt agcgaagata
    36721 actcgtttct ggcttttcat tcctattctc ccagcaagaa ctggatttaa tccagatttt
    36781 attctttacc atgaaaccaa tcccggggca aggacctttc caatatgaaa ttcgaatcaa
    36841 ggaagacgag cttcctgcaa atgtctcctt ttccgtcatt ccggcgtaag ccggaatcc
//
LOCUS       DASPRR010000523         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2063898, whole genome shotgun sequence.
ACCESSION   DASPRR010000523 DASPRR010000000
VERSION     DASPRR010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063898"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VEL68_19565"
     CDS             complement(<1..1043)
                     /locus_tag="VEL68_19565"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXZ38234.1"
                     /translation="MLMPNVTPLRAEPPLIVSLDIGTSSIRASLFDRMGRAVEGLKAR
                     KAHEIQTSSGGAAEAVPDSILQSVFDCLDQLTQKIRPLKSGIGGVASCTFVNNVLGID
                     GTDRAVTPLTTYADTRAEKEVAGLQSDFDEASVHDRTGCRFHPSYLPARFRWMARVHP
                     EWLEKVDRWVSIGEYLELKLFGVTAVSHSVASWTGLQDRHRLVWDAPLLAGLPVGVEG
                     LSSLTDVNRPRHGLRSEFAGRWPDLAEVPWFPAVGDGAAANVGSGCISSGQVAVTMGT
                     TSALRVVLSDAPEHLPSGLWCYRVDGKRSLLGGALSEGGSVFAWMKDTLNLGAYPDLE
                     KALAAVKADFHGLT"
     gene            complement(1251..1667)
                     /locus_tag="VEL68_19570"
     CDS             complement(1251..1667)
                     /locus_tag="VEL68_19570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXZ38235.1"
                     /translation="MRFFRTLIVEDNPLFRQTILEILSHQFPSMVLEAVPDGKTALEK
                     IEDSLPDLIFMDIRLSGESGLHLTEHIKRRHPQIIVTILTSYDWPEYRDAAYKFGANY
                     FLMKGSSTKREIVDLIESILNKLGFGPNGILKNSLS"
     gene            1872..2528
                     /locus_tag="VEL68_19575"
     CDS             1872..2528
                     /locus_tag="VEL68_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXZ38236.1"
                     /translation="MGEKQRIVIAEDHTILREGLRALLSSDPDFEIAGEAEDGQEAIR
                     CVEKLKPRLVLMDLSMPRMNGMDAIREIKKRVPDTKVLVLTVHKTDEYIVATLQAGAD
                     GYVLKDSTNVELRMAIKSVLEGKFFISPGISAKVVEGYLETKRPQKASTPWESLTARE
                     RGILKLIAEGYKNKEIADFLCISVKTVEKHRANLMQKLDLHSVSALTALAIEKGLIAK
                     "
     gene            complement(2554..3507)
                     /locus_tag="VEL68_19580"
     CDS             complement(2554..3507)
                     /locus_tag="VEL68_19580"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HXZ38237.1"
                     /translation="MQKSDFSNSRLIMIGGKGGVGKTTISAALAFHFAASGEKTLLIS
                     SDPTPSLSDIFETEIGSKEKRIRGVEGLYGLEISSEVILQRWKEKFGPEIYEVISAFS
                     NLDYDFVDYIGGAPGIEEEYMLHTILELVESGTYRRVIWDTAPAGHTLRLLHLPQLFL
                     NHLEAAARFYMNLYGTLEKIASTVRLKKSKRTILQIIEGWKELSQKVLDFIRDPEEAA
                     YVIVTIAEALGVRLTERLLKDFDDFRLPVGHLIVNNLVQEADCDFHRQRQAMQRKYLK
                     ALSAQYGKRMTLIEVPLFPYEVRGVKRIAQVAKVLFPSGPG"
     gene            complement(3628..4569)
                     /locus_tag="VEL68_19585"
     CDS             complement(3628..4569)
                     /locus_tag="VEL68_19585"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXZ38238.1"
                     /translation="MNYPWTELLHLGLVVQAFFPEARERKSQALEITRKLAADPFFQA
                     LEISGVEEQSLQRELAGIVRASGKSLVFSGGGYCRAGGHNLHDLEEGRREKAVQQVKK
                     IIDEASDYGCSILYVMGFEAPAPPARDRAREKFAMSLADLSDYARGKNPAAPLALSVE
                     NFYLLTDTPFLIGPTLDFARMLRELRPKHPNLGLTFDTSHILQLKEDLAATFARVKDV
                     IAHIHLSNCLLRDRSSPFYGDKHPPYGFLGSEIGIPELAGFLGTLKAHGYFLRTSSRE
                     KPILSLEVITPPGQTPEAALGEAKEAFQKAWAEFEKN"
     gene            complement(4712..5341)
                     /locus_tag="VEL68_19590"
     CDS             complement(4712..5341)
                     /locus_tag="VEL68_19590"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXZ38239.1"
                     /translation="MKRIEFSCGEITLEGMIHIPQGSGPFPLVVVCHPHPLYGGSMHN
                     NVVDAVCSSLGEKEIAWLKFNFRGVGRSGGSFGDGIGEKEDAKAALSFGERQAGIDAE
                     AIGLCGYSFGSMVAFPLAVEDDRVRVVAGIAPFVQPEHLLNQYTRPKLLACGTQDEFI
                     PSRALEKVVQKMPEPKELALFPGVDHFWWGKEGTMAERVAQFFGKYLKK"
     gene            complement(5431..5793)
                     /locus_tag="VEL68_19595"
                     /pseudo
     CDS             complement(5431..5793)
                     /locus_tag="VEL68_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            complement(5906..>6533)
                     /locus_tag="VEL68_19600"
     CDS             complement(5906..>6533)
                     /locus_tag="VEL68_19600"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ38240.1"
                     /translation="GAGSNFSAGIDFNFLAQVGRDDGRAPGIRLREEIDLIHSILNRI
                     EQVEKPVVAKIHGFCGGLGMELALTADFRIAAESATLGVPEIILGLIPDCGGTTRLTR
                     MAGPVWTKELIMTGDMFPARRIYEMGLLNEVVLDDQLEDRTRVFLNKLLQRPPLALGL
                     AKKAIDWGVGMDKNSHMEIEAYVQSLLITAKDFPQTLQKGFAQLLKKK"
BASE COUNT         1517 a         1840 c         1713 g         1463 t
ORIGIN      
        1 gtcaaaccat ggaagtccgc ctttacggcg gccagagcct tttccaggtc cggataagct
       61 cccaagttca aggtatcctt catccacgca aaaacgctcc ccccttcact caaggctcct
      121 cccagcagag accttttccc atccacgcgg taacaccaca ggccggatgg gaggtgttcg
      181 ggagcatcgg agagaaccac ccgcagagcg ctcgtcgtcc ccatggtcac agcgacctgc
      241 ccagaggata tgcatccgct tcccacattg gcagcggccc cgtcgcccac cgcaggaaac
      301 caggggactt cggccaaatc aggccagcgc ccggcaaact cagaacgcaa tccgtgccga
      361 ggacgattca catcggtcag gctggaaagc ccttccaccc ctacgggaag accggccagg
      421 agaggggcat cccagaccag ccggtggcga tcctgcagcc cggtccagga agccacggaa
      481 tgactcaccg cagtcacccc gaagagcttc aattccagat attcaccgat ggaaacccag
      541 cggtcaactt tttccagcca ctctggatga acccgggcca tccagcggaa gcgagccggc
      601 aggtaactcg gatggaagcg gcagcccgtc cggtcatgca ccgaagcttc atcaaaatcg
      661 gactgcagcc cggccacttc tttttccgcc cgggtgtcgg catacgtggt aagaggagtg
      721 acggctcggt cggttccgtc gattcccagg acgttgttga caaaggtgca tgaggccact
      781 ccgccgatcc cggattttag aggccgaatc ttttgcgtca gttgatccag acaatcaaag
      841 acggattgga ggatggaatc ggggactgct tcggccgctc cccctgaact cgtctgaatt
      901 tcgtgggcct ttcgagcctt caacccctcc acggcccttc ccatacggtc gaaaagggag
      961 gcccgaatag aggacgtgcc gatgtccagg gaaacaatga gagggggttc cgcccgaagg
     1021 ggggttacgt tgggcataag catgatctct tcctgaggcc tgattttctt cctcctttca
     1081 tccccctctt cttcccccgg tctagcccga gggggcgggt ggaaaagacc tttccgccct
     1141 gcgccgaaag gccattgatg caggacgaac cctggcagtg cgaatattat atcactttaa
     1201 agggaggacc caggtgggaa aagaatgggg aaaggaatta gagccttctt ctaggaaaga
     1261 gaattcttta gaatcccgtt ggggccaaac cccagtttgt tgaggatgga ttcgatcaaa
     1321 tccacgattt ctcttttggt ggaggagccc ttcatgagga aatagttggc cccgaactta
     1381 taagccgcgt cccggtattc cggccaatcg tagctggtca gaatggtcac gatgatttga
     1441 ggatgtctcc ttttgatgtg ctcggtcaag tggaggccac tctctccaga cagtcggatg
     1501 tccatgaaga taagatcagg aagggagtcc tcgattttct ccagagccgt tttgccgtct
     1561 ggcaccgcct ccaaaaccat ggaagggaac tgatggctca gaatctccag gatcgtctgg
     1621 cggaagagag gattgtcctc gacgattaaa gtcctgaaaa agcgcatatt ttggctattt
     1681 tcctgctcgc ggaggaattc gcctgataaa atactttact aaccgttcag ctcatggtaa
     1741 atagggtgag aacagtattt aaaacccacg gacacagtat ttctggtgtg aagtcaggta
     1801 gggcttaaag ggcctgctca ggcggcaatg agatatttga ggaggttctt ctagccggag
     1861 gtaagctgac catgggagaa aagcaaagaa tcgtgatcgc cgaggaccat accatccttc
     1921 gagaaggttt gagagctctt ttatcctcgg acccggattt tgagatcgcg ggggaagcgg
     1981 aggatggaca ggaagccatc cgttgcgtgg aaaagttgaa accccgtttg gtcctgatgg
     2041 acctttccat gccccggatg aacggcatgg acgccattcg ggagatcaaa aaaagagtgc
     2101 ccgatacgaa agttctggtc ctcacggtcc ataagaccga tgaatacatc gtcgccactc
     2161 ttcaggccgg ggcggacggg tatgttctga aggattctac gaacgtcgaa ctacggatgg
     2221 ccataaagag cgtgctggag ggaaagtttt ttatcagccc gggaatttcg gccaaagtcg
     2281 ttgaagggta tctggaaacc aaaaggcccc aaaaggcatc caccccctgg gagagcctga
     2341 ccgcccgaga acgggggatc ttaaagctga ttgccgaggg gtataagaat aaggagattg
     2401 ctgatttcct ctgcattagc gtaaagaccg tcgagaagca ccgtgccaac ctcatgcaga
     2461 agttggacct gcacagcgta tccgccttga ccgccctggc catagagaaa ggactgattg
     2521 ccaagtaacg acaaaggcta aatcgcaacc tccctacccc gggcccgaag gaaagaggac
     2581 cttcgccact tgagcgattc ttttcacccc ccggacctcg taggggaaga gtgggacctc
     2641 gatcagagtc atccgctttc cgtactgggc gctcagggcc ttcaggtact tccgttgcat
     2701 ggcttgccgc tgacggtgaa aatcgcagtc ggcttcttgg accaggttgt tcacaatcag
     2761 atgtccgacg gggaggcgaa agtcgtcaaa atctttcagc agccgctcgg tcaatcgaac
     2821 tcccagggcc tcggcgatgg tcacgattac ataagcggct tcctccgggt cccggatgaa
     2881 atcgagaacc ttctgggaca gctccttcca cccttcaatg atctggagga tggttcgttt
     2941 gcttttcttc aaacggaccg tgctggcaat cttttccaag gtcccgtaca gattcatgta
     3001 gaaccgggcg gccgcctcca gatggttgag gaagagttgg ggaagatgga ggaggcgcag
     3061 cgtatggccg gccggggcgg tgtcccagat gacccttcgg tacgttccgc tctccaccag
     3121 ttccaggatc gtgtggagca tgtattcttc ctcgatcccg ggcgcccctc caatgtagtc
     3181 cacaaaatca tagtccaggt tggaaaaagc cgagatgacc tcgtagattt cgggcccgaa
     3241 cttttccttc catcgctgca aaatcacttc ggagctgatc tccagaccgt agagaccctc
     3301 caccccccgg atgcgctttt ccttgctccc aatctcggtt tcgaagatat ccgacagcga
     3361 tggggtggga tcggaggaaa tcagtagagt cttctccccg gaagcggcaa aatggaaggc
     3421 cagcgcggct gaaatggtcg ttttgcccac tcctcctttt ccgccaatca tgatcagccg
     3481 ggaattagaa aaatcgctct tctgcatggt caacctgcta caagtttcga ggtccgggtt
     3541 cgggtttcga gtcagcagaa ggttcccatt ccgcttctct tttcgcagtt ccaactcgaa
     3601 tcgaaacctg aaacccgaaa caataagtta attcttttca aattccgccc aggctttctg
     3661 gaaggcctct ttggcttctc ccaaagccgc ctctggagtt tgccccggag gagttatgac
     3721 ttccaggctg aggattggct tttccctaga agaggtccgg agaaaatacc catgggcctt
     3781 cagcgtcccc aaaaagccgg ccagttctgg aataccgatt tcgcttccca aaaagccgta
     3841 ggggggatgc ttgtccccgt aaaaagggga cgaccggtct cggagcaagc agttgctcag
     3901 atggatgtgg gcgatcacgt ccttgacccg ggcgaaggtc gcagccaaat cctctttcag
     3961 ctggaggata tggctggtgt caaaagtcag gccgaggttg gggtgttttg ggcggagttc
     4021 cctcagcatc cgggcaaaat ccagggtggg cccgatcaga aaaggcgtgt cggtcaggag
     4081 gtaaaagttt tctactgaca gggcaagcgg cgcggcaggg ttttttcccc gggcgtagtc
     4141 gctgaggtcg gccaaagaca tggcgaattt ctcgcgcgcc cggtcacggg ccgggggggc
     4201 cggggcttcg aagcccatga cgtaaagaat cgagcatccg taatcagagg cttcatcgat
     4261 gatcttcttg acctgctgga cggccttttc tcttcggccc tcctcgaggt cgtggaggtt
     4321 gtgcccgccc gcccggcagt agcctccccc tgaaaaaacg aggctctttc cgctcgcccg
     4381 tacgattcca gccagttctc tctgtaaaga ttgctcttcc actcccgaaa tctccagagc
     4441 ctggaaaaaa gggtcggcag ccagcttccg ggtaatctcc aaagcctggc ttttccgctc
     4501 tcttgcttcg gggaagaaag cctggaccac aaggcccagg tgaagaagtt cagtccacgg
     4561 ataattcata ttctcctcca gtgtggaggt ttctagtcgc tggctgctag tcactaatca
     4621 tggtaccaaa cgcccgtcct aaacgatcca cgcagaaccg gaaacaaaga ccagaaacga
     4681 gtaatcagca accggccacg agcaacgtcc tttatttttt cagatatttt ccaaaaaact
     4741 gtgccactct ttcggccatc gttccttctt ttccccacca gaaatgatcg acccccggaa
     4801 agagggccag ttccttgggt tcgggcatct tttgaacgac cttctccaag gccctggaag
     4861 gtataaactc atcctgtgtc ccgcaggcca agagcttcgg tcgggtatat tggttgagga
     4921 ggtgttcggg ctggacaaaa ggagcaatgc cggcaaccac ccggacccgg tcgtcttcca
     4981 cggccaacgg aaaagcgacc atggacccga aggagtagcc gcataacccg atcgcctcgg
     5041 catcaatccc agcctgcctc tccccgaagg agagggccgc cttggcatcc tccttttccc
     5101 cgatcccgtc tccaaaactc cccccgctcc tccccactcc ccggaaattg aatttcagcc
     5161 aggcgatttc tttctctccc agggaagaac agacagcatc caccacgttg ttgtgcatgc
     5221 tgcccccgta tagcgggtgg ggatgacaca ccacgactag ggggaatggt cccgaccctt
     5281 gggggatatg gatcattcct tcaagagtaa tctcgccaca agaaaattct attcttttca
     5341 tttcttcacc acagaggcac agaggacaag gagtaatata ttttgaaaat aagccaaggg
     5401 aaaaacaaaa cttctgcccg gccagttcga aattcgcttt aatccatctt ttaagaccgg
     5461 cacgttgaaa ttaatcaaca ggccgacttt cataccggtc attttcagat aggtcaaaag
     5521 ctgggcttgg tgtatgggca acaagctctc acaagctttt aattcaagta tcaatcggct
     5581 ttctacgatt atgtcgagac gatatccgca atccaaccta aggcctttgt attcaatcgg
     5641 cagttccttt tgcctttcaa actggatttt cctcaaaccc agctcgcggc acaaacattc
     5701 ttcataagtc gattccaaaa gccccggccc gagtatctta tgcacttcaa tcgccgccgc
     5761 aatcaccttg cctgtacggt tatttatatc catcctaccc ttcctatttt caattcccta
     5821 aaaaggaaaa tttaccaagg ggtgcggagg atttatatcc ctaaatacct ctgtgctctc
     5881 tgtgtctctg tggtgatcga atttctcatt ttttcttcag gagctgggca aagcccttct
     5941 gcaatgtctg cgggaaatct ttggccgtga tcaggaggct ctggacgtag gcttcgatct
     6001 ccatgtggga atttttgtcc atccccactc cccagtctat cgctttcttg gccaggccga
     6061 gggccagggg gggccgttgg agcaatttat tcaggaagac ccgagttcgg tcctccagct
     6121 ggtcatcgag aaccacctcg ttcagtaatc ccatttcata aattcttcgg gcgggaaaca
     6181 tatccccggt catgattaac tctttcgtcc agaccggccc ggccatgcga gtcagacggg
     6241 tcgttccgcc gcaatccggt atcaagccca ggatgatctc cggcaccccc agggtggcgc
     6301 tttccgcggc gattcgaaaa tccgcagtga gagccagctc catccccaat cccccgcaaa
     6361 aaccgtgaat cttggcgacc accggctttt cgacctgttc gatccggttc aaaatggaat
     6421 ggatcaggtc gatttcttcc cgcagccgaa tccccggggc ccgcccgtcg tcgcgaccca
     6481 cctgggcaag gaaattaaaa tcaatccctg cagagaaatt gctgccagct ccc
//
LOCUS       DASPRR010000524         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_128370, whole genome shotgun sequence.
ACCESSION   DASPRR010000524 DASPRR010000000
VERSION     DASPRR010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128370"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VEL68_19605"
     CDS             complement(<1..347)
                     /locus_tag="VEL68_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38241.1"
                     /translation="MSLTQLIKEKALELGFVAVGVTTPEPFTLYAEEIASRPEMYAWG
                     RGLGQRASVRDLDLTRFVDPVRYLPEVKSLIVVTDSYFEEDFPPSLAGKIGRCYLKGL
                     FCPEETSHSRRRR"
     gene            complement(429..2081)
                     /locus_tag="VEL68_19610"
     CDS             complement(429..2081)
                     /locus_tag="VEL68_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HXZ38242.1"
                     /translation="MKFGIRCSIFFLLLTFSLGCGPEKGEGPSSEKEPSLPEKALPAF
                     GDLLIQGSIGDASNLIPMLASDSSSHGIAGLIFNGLVKYDKDLNLVGDLAESWEVSPD
                     GLTITFKLRRGVKWQDGKEFTAEDIRFGYQTIINPNTRTAYSGDYREVKEARLIDSHT
                     FQVTYQRAFAPGLASWGPLVVLPKHLLEGQDINTTPFSRKPVGTGPYKFKEWKTGEKI
                     VLEANPGYFEGRPYIDGFVYRIIPDPATMFLEMQAGGIDFMGLTPLQYKRQTESPRMR
                     RDFRKYQYLSFGYTYLGYNLKDWKFQDRRVRQAITHAIDKKEIIEGVLLGLGLIATGP
                     YKPDTQWYNPRVKEYPYDVEKAKKLLAEAGWKNSGNEGVLEKDGKPFEFTILTNQGNE
                     SRARCAEIIQRRLAAAGIKVKIRTVEWAAFINDFIDKKNFEAVILGWTLGQDPDIYDI
                     WHSSKVGQKELNFISYKNPEVDSLLEKGRYTFDYKIRKACYDRIQEILAEDQPYTFLF
                     VPNALPILHARFHGVEPAPAGISYNMDKWYVPKAEQRYVLKP"
     gene            2271..3212
                     /locus_tag="VEL68_19615"
     CDS             2271..3212
                     /locus_tag="VEL68_19615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXZ38243.1"
                     /translation="MKSGKEPRRTFRGVRALLEEIPRKIKIVVNPNAGGGKGRRIFPL
                     LRQKLLDRRVSFHLQFAESADHVTHLCRQALGEGYNYIISCGGDGTHHQALKALAGSR
                     YILGFIPAGTGNDFPANLGIPEDLDSACDLLLKGKVRKIDVIQVNDGEYMAGVGGIGF
                     DSEVNAIANRISRFVGGRTAYVLPVLIKTLTYRPKEISLHMDDEQTQGRALMVAFGNI
                     KSYGKGMQITPLAEPDDGLLDICWVDPVNTFRLYRFFPTVFRGEHIEMPEVHYYRATT
                     VQVETSVPMDLYGDGEYICKTPFTLRVLPQSLRVLVP"
     gene            3327..>3599
                     /locus_tag="VEL68_19620"
     CDS             3327..>3599
                     /locus_tag="VEL68_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXZ38244.1"
                     /translation="MSSRPIYTVSQLTGEIKTLLEKNFENIWVEGEISNFRHPTSGHL
                     YFTLKDESCQLRAVMFRMQNRLLKFDPEDGLQIVCYGRLTVYDPRGE"
BASE COUNT          803 a          973 c          976 g          847 t
ORIGIN      
        1 tccctcctcc gccggctgtg ggaggtctcc tccgggcaga agagcccctt caggtaacaa
       61 cggccgattt tgccggccag ggaggggggg aaatcttctt cgaaatagct gtcggtcacc
      121 acaattaggg atttgacctc agggagatac cggaccgggt ccacgaaccg ggtcaggtcc
      181 aggtctcgga ccgaggcccg ctggcccagt cctcggcccc aggcgtacat ctccggccgg
      241 ctggcaattt cttccgcata gagagtaaat ggttcgggcg tagtcacgcc gacagctacg
      301 aaacccaact cgagggcttt ctcttttatg agttgagtca gggacataaa acaactacct
      361 cccagagcaa agaaaccggg gcatggcgta aactgctctt tttctttgct ctttgcgctc
      421 cgcgtttttc agggcttcag cacgtacctc tgctccgcct tcggtacata ccatttgtcc
      481 atgttgtagg aaatccccgc cggtgccggc tccacgccat gaaatctggc gtggagaatg
      541 ggcagggcat tggggacgaa cagaaaagtg tagggttggt cctcggccag gatttcctgg
      601 atccggtcat aacaggcctt gcgaattttg tagtcgaagg tatatcttcc cttctccaga
      661 agggaatcta cctcggggtt cttgtaggag ataaaattca actctttctg gcccaccttg
      721 ctggagtgcc agatgtcata gatgtccggg tcctgcccca gggtccaccc cagaatgaca
      781 gcctcaaaat tcttcttgtc aatgaagtcg ttgatgaagg ctgcccattc caccgtccgg
      841 atcttcacct tgatccccgc cgcggccagc cgtcgctgga tgatctccgc acaccgagcc
      901 cgggattcat ttccttggtt ggtcaggatg gtgaattcga agggtttgcc atccttttcc
      961 aggactccct cattccctga gttcttccac ccggcttcag ccagaagctt cttcgctttc
     1021 tccacatcgt aaggatactc tttgaccctg gggttatacc attgggtatc gggcttgtag
     1081 gggccggtgg cgatgagccc caggcctaat aggactcctt caatgatctc tttcttgtca
     1141 atggcatggg taatggcctg acgcacccgc cggtcctgaa atttccagtc cttgaggttg
     1201 tatcccaggt aggtgtagcc gaacgagagg tattgatatt tgcggaagtc tcgtcgcatt
     1261 cttgggcttt ccgtctggcg cttgtattgg aggggggtga gacccataaa atcgattccc
     1321 ccggcctgca tctccaggaa catggtagcc gggtcaggaa taattcggta cacaaaacca
     1381 tcgatgtagg gacggccttc aaaataaccg gggttggcct ccagcacgat cttttcgccc
     1441 gtcttccact ccttgaactt atacggcccg gtaccgaccg gtttgcggct gaaaggggtg
     1501 gtgttgatgt cctgtccttc caggaggtgt ttcgggagca caaccaatgg gccccagctg
     1561 gcgagtcccg gagcaaaagc tcgttgatag gtaacctgga aggtgtggga atcgatcagc
     1621 ctggcttctt tgacttcccg gtaatctccg gagtaagccg tccgggtgtt ggggttgatg
     1681 atcgtctggt agccgaatcg aatatcttcg gcagtgaact ccttgccgtc ctgccatttc
     1741 acccctcggc gcagcttgaa ggtgatggtc agcccgtccg gggaaacctc ccaggattct
     1801 gccaggtctc ccaccaggtt caggtcttta tcgtacttca ccaaaccatt aaagatcaga
     1861 ccggcaattc catgggagga gctgtccgaa gccaacatgg ggatcagatt gctggcgtcc
     1921 ccgatcgacc cctggatcaa aaggtcccca aaggccggca atgctttttc gggcaagctc
     1981 ggctcttttt cagaagaggg accttccccc ttctccggac cgcagcccag ggaaaaagtc
     2041 agcaaaagaa aaaaaatgga acacctgatt ccaaatttca taagacggag cagccgaaag
     2101 cggaaggatt gtggaacaaa atccgaaagt atccggtttt attttctttt ttggcttgag
     2161 aggaacaagt cggagccctc tttaaaaggt accctacccc cccctctatt tcaagacaaa
     2221 aagattcggg ctaaaaaatc ttgtaaaagg ctcgataatt tgataggaag atgaagtccg
     2281 ggaaagaacc ccgccggacc ttcaggggag tgagagcttt gttggaagaa attccgcgaa
     2341 agatcaaaat tgtcgtcaat ccaaacgccg gggggggcaa aggtcgaaga atcttccctt
     2401 tgctccggca aaaacttctg gaccggaggg tgtctttcca tctccaattt gcggagagcg
     2461 cggatcacgt cactcatcta tgccgccagg ccctcggaga aggatacaac tacatcatct
     2521 cctgcggggg ggacggtact catcaccagg ccctcaaagc cttggcgggc agccggtata
     2581 tcctgggttt tatccccgca ggaaccggga atgatttccc tgccaacctg gggatcccgg
     2641 aggacctgga ttctgcctgc gaccttctcc tgaaaggcaa ggtccggaag atcgatgtga
     2701 ttcaggtcaa cgacggagaa tatatggccg gggtgggggg tatcgggttt gattcggagg
     2761 tcaacgccat cgccaatcgg atcagtcgtt tcgtgggagg ccggacggcc tacgttctgc
     2821 ccgtattgat caaaacgctc acctaccgcc cgaaggaaat ctctctgcac atggatgatg
     2881 agcaaaccca ggggagggcg ctcatggttg ctttcggcaa tatcaaaagc tatgggaagg
     2941 gcatgcagat aacccccctg gccgaacccg atgacgggct cctggatatc tgctgggtcg
     3001 acccggtgaa caccttccgc ctctaccggt ttttccccac ggtcttcagg ggggagcata
     3061 tcgaaatgcc ggaagtgcat tactaccggg ccacaaccgt tcaagtggag acctcggttc
     3121 ccatggatct ctatggagac ggagagtaca tttgcaaaac ccccttcacc cttcgtgttc
     3181 ttccccagtc cctgcgggtg ttggttcctt aaaagcagga tacaggagtc agggatcggg
     3241 attcgggaga aagatttaga tattatcaag gtaatattcc attttccatt ttgcattgcg
     3301 catcctgaat cccggggcct attgctatgt cttcccgtcc aatttatacc gtctcccagc
     3361 tgaccgggga aatcaaaacc ctcctggaaa agaactttga aaacatctgg gtggaggggg
     3421 aaatttccaa cttccggcat ccaacctcgg gtcatctgta cttcaccctg aaggatgagt
     3481 catgccagct gcgagcggtc atgttccgga tgcagaaccg gctgttgaaa ttcgatccgg
     3541 aagacggcct ccagattgta tgctatggaa ggctgaccgt ttatgatccc cggggcgag
//
LOCUS       DASPRR010000525        22886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_273344, whole genome shotgun sequence.
ACCESSION   DASPRR010000525 DASPRR010000000
VERSION     DASPRR010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 22886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22886)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22886
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273344"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..860)
                     /locus_tag="VEL68_19625"
     CDS             complement(240..860)
                     /locus_tag="VEL68_19625"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HXZ38245.1"
                     /translation="MKSLLVLISVLLLATGLAAQERRESWNFDADETAKIAKGFSNEV
                     GEWKVVADSTAPSPPNVLAQLAKNSGSTFNVALVSDTNYKDLDISVKMKAVAGKEDQG
                     GGLIWRAKDAKNYYVARYNPLEDNYRLYKVEKGRRSQLKSADIKRTEGWHTLRVTMKG
                     DLIQCHYDGQKYLEAKDNAFPGEGKIGLWTKADAKSHFDDLAISGQ"
     gene            complement(1049..1876)
                     /locus_tag="VEL68_19630"
     CDS             complement(1049..1876)
                     /locus_tag="VEL68_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ38246.1"
                     /translation="MKQPLKNVNLVIGGTLVLAILVTASLSFFYTPYDPNRMNMAKRF
                     QSPGEEYLLGTDQYGRDILSRVMKGAVNSLIVGWVAVGIGMGFGILFGSLAAFWRGWK
                     DEGVMRLMDVLYGFPAVLSAILITSILGPGMVNSMLAIGVFNIPIFTRLTRGTSLSVW
                     QRDFVTSARAVGQRNGRIIWNTVLPNILSPLIVQGTVQFAIAILAEAALSYLGLGTQP
                     PHASWGRMLNEAQTFMEISPWLAIFPGLAIAWAVLGFNLLGDGLRDLLDPKLSRTRR"
     gene            complement(1881..2837)
                     /locus_tag="VEL68_19635"
     CDS             complement(1881..2837)
                     /locus_tag="VEL68_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ38247.1"
                     /translation="MAYQFIKRLLILLATLLLVSGVIFLVLQVIPGDPAQIILGIQAT
                     PENLQALRHKLGLDLSLPVQYWNWMSGVFRGDLGVSITYDVSISELILSRLAVTFPLA
                     LLSIFFAVVFSIPLGVYAALHRNRPGDYGVMVFSQIGLAVPAFWAGILLILFFAVYLQ
                     WFSAGGFKSWMESPLGALKSLLLPALSLGLIRAAVLARLTRSCMLEALGEDFIRTARA
                     KGLAERVVVYKHALRNALIPVVTIVGLQMGELLAGAIVIENVFNLPGLGRLIFLAIGQ
                     RDLPVVQGVGLLIAFFIVIVNFAVDVIYGVVDPRIRSGSEEI"
     gene            complement(3017..4543)
                     /locus_tag="VEL68_19640"
     CDS             complement(3017..4543)
                     /locus_tag="VEL68_19640"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38248.1"
                     /translation="MAIHSRQGKWVGLLLIFFCFVIWGAFSSEAIAQRPVPGGTLTIA
                     LAAEPSGLDPTTNPSAAIKRVVHYNLLEGLLKVDRNGRVAPALAKGYSVSKDGKEYTF
                     VLHSGIKFHDGTPCTAEDVKFSLERLLDPKTAAPYRMYYESIESIQVVDSTTVKFKMK
                     KYDSNFLFNMARGDAVIVSKQSVDRLKSAPVGTGAFKFVEWKRGDSVIMAKNPDYYVK
                     GLPYLDKVTFKFIPDPSAQLAALRAGDVDVISYDLAPENVPALEKDGRFKVLKGNTTT
                     DVIMAMNHSRTPFSDPKVRQAITLAIDRKAVIQGAVAGYGTPIGSHMDPTNPYYVDLS
                     GLYPYNPEKAKQLLAEAGYPTGFGAVLKLPEPYAYARRSGEIIADQLSKVDIKLIIEV
                     IPMGQWVDRVFKNADYDLTVMGHAEPFDIEIYSRPNYYFRYNNPMFQDLIKKAEGEMN
                     EQARKKTYEQAQRMLADDFVNVFLFIYPALPAMKKEVMNWWKDYPTIVVDASEVYIQK
                     "
     gene            complement(4649..6253)
                     /locus_tag="VEL68_19645"
     CDS             complement(4649..6253)
                     /locus_tag="VEL68_19645"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ38249.1"
                     /translation="MILWGGNIITVDARRPAARAVAIKDGRILKVGSDEEVKALAGNK
                     TRRVALRGRTVTPGFIDSHQHLSQYGTDLLQIDCSPKRCRTIEDIQKAVLLEAKRKPA
                     GEWVRGVGYDDTKTPAGRTLSRWDLDEVAPDHPVLIQQISGHWGVANSKALEIGGVRE
                     DAGDPKGGAYGRDAGSGRLNGILYEQAEFAFVFEGTTGRPPIIPPFSLKDRLRGMKLA
                     ADRYLASGITSVHDALVSAKTLETYQEARKKGDLKLRVYMLISIEYLPQLRGLNLKTG
                     FGNEWLKIGGVKILADGGIAGRTAYLSEPYVGSEDRGILALESEQVLHELIRQGHSAG
                     FQVCVHANGDRVIEMALDGFKKALDEFPRKDHRHRLEHCTVVNPEILKRIKKLRILVT
                     PFGSYIYHHGEKMIPHYGARRVEMMFAHRSFLDYGIAVSGASDSPCGPYEPLLAIQSC
                     VTRKSAMGELLGDRQRISVEEAISLYTKASSYASFEEDLKGSITPGKLADMVVLGKDP
                     RKVDPDEIKDIPVEMTIVGGEILHRA"
     gene            complement(6417..7745)
                     /locus_tag="VEL68_19650"
     CDS             complement(6417..7745)
                     /locus_tag="VEL68_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ38250.1"
                     /translation="MSQSDVKRYDKEYVVHSWSVQSALDPLVITKAQGVHMWDENGKR
                     YIDMTSQLMNQNIGHQHPKVVQAIKDQAEKLCFVAPGVAYESRSFLGKALAEVTPGDL
                     KRFFFTLGGADANENAVKIARMYTKKWKIIARYRSYHGATYGAIALTGDPRRPAVEPV
                     MPGVVHVFDPYCYRCSFGLTYPACNLRCADSVEEVIGYETPETVAAVLVESVVGSNGL
                     IVPPDGYMQRLREICTRNKVLLIADEVMAGFGRCGKWFAIDNWNVVPDMITMAKGLTS
                     GYVPLGAVAISRKIMEVLDKQMLWAGLTYNAHPLACGAAIATLKVYREDKLMDNAIAM
                     GEIIRKETEAMKARHKCLGDARGIGLFWALELVKNKKTREPLVKWNAMGPDGAVAKEI
                     NKRLLEGGVYTTVRWNFLFVVPPLCIKENDLREGLAVIDKVLDYADTLAE"
     gene            complement(8025..8762)
                     /gene="truA"
                     /locus_tag="VEL68_19655"
     CDS             complement(8025..8762)
                     /gene="truA"
                     /locus_tag="VEL68_19655"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXZ38251.1"
                     /translation="MRNIKLVIEYDGTNYHGWQVQPNGLTIQEVIEEKIQTMTRQRVR
                     LTGSGRTDAGVHALGQVANFRTDSTIPVEGFHRGLNSLLPPDIAIQSVEEVDLQFHAQ
                     YAAKRKTYRYVILHREVPSALFRNYSWRVPGALDVPAMEEASRLLTGRQDFTSFRGAD
                     ADTEDPVREVFQCGWSKKDSDFLHFTMEADGFLKHMVRNIVGTLVEVGKGKRSPEQFG
                     QVLRARDRRQAGMTAPPQGLFLVEVKY"
     gene            complement(8842..10023)
                     /locus_tag="VEL68_19660"
     CDS             complement(8842..10023)
                     /locus_tag="VEL68_19660"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXZ38252.1"
                     /translation="MRDVVIASAVRTAVGTFGGALRDIPAVELGKIAVQEALKRAKMK
                     PEQVEEVILGNVLTAGQGQNPARQVLIRSGIPKEIPATTINKVCASGLKSVMMAAQVI
                     KAGDADIVMAGGIESMSMAPFLLSGARWGYRMNDGALIDGMIHDGLLDIFNRYHMGIT
                     AENVAEKFGVSRQEQDALAFRSQQNAGRAIKEGRFKEEIVPVMIPQKKGDPVAFQVDE
                     HPRPNTTMEALAGLRPAFKKDGTVTAGNASGINDGAAAIVVMSAEKAKALGVEPLAKI
                     KAYATAGVAPDIMGTGPVPSSQKALAKAGLKVKDLDLIEANEAFAAQAVYVNRTMGWD
                     IEKVNVNGGAVALGHPIGASGARILVTLLYEMKKRKAHYGLATLCVGGGMGGALIAEM
                     P"
     gene            complement(10188..11633)
                     /locus_tag="VEL68_19665"
     CDS             complement(10188..11633)
                     /locus_tag="VEL68_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HXZ38253.1"
                     /translation="MIKTKEDYIESLKKLKHVVYYRGKRVEDVTAHPAFVPHINAASK
                     TYELALKPEHEDLMTATSHLTGKRINRFTHVHQSRDDLIKKVQMLRLISHETGSCYQR
                     CVGFDAMNSLYSVTYEIDEKHGTSYHDRLKKFVAYIQENNFMVVGSMTDPKGDRSKGP
                     SQQADPDLFTHVVKKTEEGVVIRGAKAHQTGAVNSHVMLIMPTQALRPEDQDYAIACA
                     VPVNAPGVTMIFGRQTNEERKLEQGMDAGNPEFGLVGGEALVVLEDVFVPWERVFMCG
                     EVEFAGLLVERFASLHRQNYGGCKGGLTDVLVGATALAADYQGTSQASHVRDKLAEMT
                     HLAETIYTGSIACSAMGHKTKAGNWEPDTLLANTTKYNVTKNIYEVARLAHDVAGGII
                     ATLPFEDDLKNPEVSKYVEKYLKGVKDIPTEDRIRLLRLIENMTGGTALVESMHGAGS
                     PQAQKVMYMRAGNIEQKKKLAKKLAKIADKG"
     gene            complement(11717..12862)
                     /locus_tag="VEL68_19670"
     CDS             complement(11717..12862)
                     /locus_tag="VEL68_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXZ38254.1"
                     /translation="MDFELTEEQKMVQAMARDFADQEIKPNAQELDQTERHPAEIIQK
                     MAELSLMGIAIPDTYGGGGADIVSYVVAMEEISRGCASVGVIMSVNNSLVCDPIHTFG
                     TEAQKQKFLTPLASGKKLGCFGLTEPEAGSDAAAQKTTAVLQGDQWVINGKKNFITNG
                     NVADYCVLMAMTDKKMGYKGISSFIMDCKAPGFSVGAVEKKLGIKASGTAELIMEDCR
                     IPKENLLGQVGQGFLVAMNTLDGGRIGIASQALGIARAALEAAVEYSKTRVQFGAPIS
                     KLQAIQWMIADMATDLDAARLLTLRAAFLKDHKQRYEKEAAMAKLFASEAASRIATKA
                     IQVHGGYGYIKEYNVERHFRDARITEIYEGTSEIMRLVISSNILKGK"
     gene            13376..14173
                     /locus_tag="VEL68_19675"
     CDS             13376..14173
                     /locus_tag="VEL68_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38255.1"
                     /translation="MGHKIRTLTAALLVLSCLGIWGTVAKRNAQAGQTLDLSNVMARD
                     QIRACGFIAPKMILSLGSIVGSQDASGNLSEGDLVYIKLEPGKQVKAGDRLAITRVVK
                     EIEHPLTRVKLGYQVSFPGRAVILDGRGQIVPAKIEKSFHPIRYGDLVTALPSSSPSE
                     VAVRMPDKVKGTVVAAAQEEENISERGVVFIDRGSQDGVIMGDLFTVYQFPYYTDEAL
                     EKEPKLPLMKVGEGVVIYLEKETSTLLLTHSSQSVYVGDTVVLGKGK"
     gene            14283..15377
                     /gene="dprA"
                     /locus_tag="VEL68_19680"
     CDS             14283..15377
                     /gene="dprA"
                     /locus_tag="VEL68_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXZ38256.1"
                     /translation="MESKGLYHWVALSMAPGVGSILFKRLTEAFGGPEGVFQARAKDL
                     ERVEGIGPKVIEALKRFHWKEQVDKELRCLEGIGARLLTWSDEEYPPGLKQIYDPPPL
                     LYVRGCIKAQDQRAVAVVGSRYPTTYGKMFAERISLGLSQRGVTVVSGLARGVDSDAH
                     RGALAAGGRTIGVLGCGIDLIYPPENEKLFGEVAGQGAILSEFPLGTPPEKDHFPIRN
                     RVISGISMGVVVVEATLRSGSLITARFALEQGREVFAVPGNIDSARSAGTNRLIREGA
                     KLVARVEDILEEISLPALPVQEETTPKPKLTEEEARVFSALGPQAAHIDQVIAQSALP
                     SARVSVILLSLELAGHVKQLPGMRFVKTDF"
     gene            15700..18141
                     /gene="topA"
                     /locus_tag="VEL68_19685"
     CDS             15700..18141
                     /gene="topA"
                     /locus_tag="VEL68_19685"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXZ38257.1"
                     /translation="MSKPLVIVESPAKARTISNYLKNEYIIKASVGHIKDLPESRLGV
                     DIEKGFAPEYQVIKGKVKVLKEIKTASQKAKEIYIATDPDREGEAIAWHIAEEIHQPA
                     KEIHRVLFNEITARSVREAMLKPERLNQDKFEAQQARRILDRLVGYLISPLLWDKVRR
                     GLSAGRVQTVAVRLICEREEEIRKFVPQEYWSIEASLEGKNPPPFKAGLFRIDSEKAE
                     IKTGEQAQSILKDLSSLPFVVKQIERKERRKNPAPPYITSQLQQEAWRKLRFTAKKTM
                     SIAQRLYEGVELGQEGPVGLITYMRTDSVRIAPEALTAVRQFIQEKFGADYLPEKPNF
                     YKSRKTAQEAHEAIRPTSMQYEPERVKSFLTKDQLSLYDLIWNRFVACQMNPAVYDQT
                     TVDIEAGKYTFRATGSVLLFPGFMALYIEAVEENGNGEEKEGKLPELAEGEILKLLEL
                     TPEQHFTQPPPRYSEATLIRELEEKGIGRPSTYATILDNIQEREYVAKEKGRLFPTEL
                     GCLVTDLLVMNFQDIFDVQFTAQMEDELDKVEEGELRWTQALEDFYHPFQKDLEKAKV
                     EMEDIKWKGIATDLTCEKCGAPMVIKLGRHGQFLACSNYPECKNTKEFQRNEAGEIQV
                     IEAEPMQESCEKCGAPMVIKTGRFGKFLACTNYPKCKNTKKMMVNGEGKLEVAQEEVS
                     SEVCENCGAPMVIKAGRFGKFLACSKYPECKTTRRIGAEGKKGEASKAEPTGEDCEKC
                     GAPLVYRKGRFGPFIACSNYPRCRYTQKIAKGEKAVGLPAEAASAANPEQKKPEAAKE
                     EKKEGAEKKTRKKKA"
     gene            complement(18224..18640)
                     /locus_tag="VEL68_19690"
     CDS             complement(18224..18640)
                     /locus_tag="VEL68_19690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXZ38258.1"
                     /translation="MKLMLFMFLTTFFWGMAAIFDKLALGKTSPLTGMMIRQFILTGI
                     LLVVGVGSGRLGSLAALDWRSTIYFGLSGICGGALGLWTYYHALRLGGASLVVPITAT
                     YPLITVLLSWLILQEGLTVSRVIGTALIVVGVWLVK"
     gene            complement(18826..20685)
                     /locus_tag="VEL68_19695"
     CDS             complement(18826..20685)
                     /locus_tag="VEL68_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXZ38259.1"
                     /translation="MSNGAFPISMRDMKFILYEWLGIERLCQFDKFKDYSRETFDMVL
                     DQAAQLSAEVIAPLNAFADKEGCTYEKGKVKVPEAFHDAFKKYCEGGWIAASVDAEAG
                     GQGLPDSVALAAAEMFVGACCSFTTYPGLTRGCANLIESFGTPEQKKTYLEKMYAGEW
                     TGTMCLTEPQAGSAVGDVKCAAKKVGDHYLIQGTKIFITAGDHNLTPNIIHAVLARAE
                     NAPPGIKGLSLFVVPKVRINADGSLGEPNDVNCGGIEHKMGLKGSATCTLNFGDDGKC
                     HGYILGQEGQGIQLMFQMMNEARLGVGLQGFALGNLAYLYALKYAKERVQGVDIRKMK
                     DPNAPRVTIINHPDVRRMLITMKAYAEGLRALIYKTAYYADLAKVATDPKEKEICENT
                     IDLLIPVVKAYSTDMAFRMAEWAIQIHGGYGYCGEYPVEQLCRDVKITSIYEGTNGIQ
                     AMDLVGRKLSLKKGALVMGWMKEMNEFIEKHKGHPTLGSFVTQLEQAKNSLVNTAMHF
                     AKTAATGDPLYPMLQCVPFLEMFGEVQLAYLLLDQSLVAKEKLQALYDKAGAKTPEAQ
                     AKVIEEQADAAYYSGKVYGTEFFVTNILPQVQAKAATVLGGNRSALAIQEAAF"
     gene            21359..21955
                     /locus_tag="VEL68_19700"
     CDS             21359..21955
                     /locus_tag="VEL68_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38260.1"
                     /translation="MSRLTTPSKIQAFLDDLEYSTEEIYRCPLRVLRERVAHCYDGAL
                     FAAAMLRRIGYLPFILEMLPNSRDDDHLLALFKKEMHWGAVGKSNFVGLRFREPVYRT
                     VRELVMSYFEQFYNLEREKTLRAYTVPLNLQSFDRHGWMIKDEPLYWIAEKLDRTRRV
                     RVLTPKMVRNLSLLDPRAYQAGLMGADQKGLFHPRRKG"
BASE COUNT         4928 a         6454 c         6265 g         5239 t
ORIGIN      
        1 ggatgggcta agtacttcat aggagccttg agaacgtgaa acggtcagca gcccaagctc
       61 gatctcattt tctcgttccg agacgaccgc cgcatccgtg gagacgaaag ggcgataggc
      121 atgggcggga tatcgggcga aaatgagaac aaccccaaga ccgaccaata gccgtgttat
      181 aaaccttgcc tttcttgtgg ctttctgccg tatcaacccc acccctctgg tgaaaagctt
      241 cactgaccac tgatcgccag atcatcaaaa tgacttttcg catcagcctt ggtccacagg
      301 ccgatcttcc cctcacccgg gaaggcattg tccttggctt ccaaatattt ctgcccgtca
      361 tagtggcatt ggatcaggtc gcctttcatg gtcacccgca aggtgtgcca tccctccgtg
      421 cgcttgatat cagcactctt caactgcgat cgtcgtcctt tttcaacttt gtaaaggcgg
      481 taattatctt ccagggggtt gtagcgagcg acgtagtagt tcttggcgtc tttcgcccgc
      541 cagatcagac caccaccctg atcctctttc ccggcaacgg ctttcatctt gaccgagatg
      601 tcaaggtctt tatagttcgt atcgctcacc agggcgacat tgaatgttga tcctgaattt
      661 ttggccagct gagccaacac gtttggtggg gacggcgcgg tcgaatccgc aacaactttc
      721 cattcgccaa cttcattgct gaaaccttta gcaattttcg ccgtttcgtc ggcatcgaag
      781 ttccacgact ccctcctctc ctgagcggcg agtccggttg ccagcaggag tacgctgatt
      841 aaaaccagaa gactcttcat ttatttattc ctcctctcta aaaattatgt ctttgatgcg
      901 tagagcatgt cgaatatggc catacccttt tcaaggactt ctttgtcact ccttccactg
      961 cctgtctggg taatccctat tcttttcagc ccagctcgtt ggattgatta gtaaacgacc
     1021 attcccggtg cctcaggaag ttcttgaatt accgcctcgt ccgggagagc ttaggatcga
     1081 gcagatcgcg gaggccatcg cccaaaaggt taaagcccaa aaccgcccag gcgatggcca
     1141 ggccggggaa gatggccagc cagggggaga tttccatgaa ggtctgggct tcgttgagca
     1201 tccggcccca gctggcgtgg gggggttggg tgcccaatcc aagataactc agcgccgcct
     1261 cggctaggat ggcgatggca aactggaccg tgccctgcac gatgagggga gacaggatgt
     1321 tggggagaac cgtgttccaa atgattcttc cgttcctctg tccgacggcc cgggccgagg
     1381 ttacaaaatc cctctgccag actgaaaggg aagtcccccg ggtgagacgg gtaaagatgg
     1441 gtatgttgaa gacaccgatg gccagcatgc tgttcaccat gccaggcccg aggatcgagg
     1501 tgatgaggat ggcgctgagc actgcgggga agccgtaaag cacatccatc agccgcatga
     1561 ctccttcatc tttccatcct ctccaaaaag ccgccaggga accgaaaaga atcccgaacc
     1621 ccatcccgat tcccaccgcc acccagccca caatgagtga attcaccgcc cccttcatga
     1681 cccgactcag gatatctcgc ccgtactggt cggtccccag caggtactcc tctccggggg
     1741 attggaagcg cttggccatg ttcatgcggt tggggtcata aggggtatag aaaaagctga
     1801 ggctggcagt gaccaggatg gccaggacca gagtccctcc gatgaccagg tttacatttt
     1861 tgagcggctg cttcatgccc tcaaatttcc tcgcttcccg accgaatgcg cgggtccacg
     1921 accccgtaga ttacatccac agcaaaattc acgatcacaa tgaaaaaagc gatcagcagg
     1981 ccgaccccct ggaccaccgg caggtcccgc tgaccgatgg ccaggaagat caggcggccc
     2041 agaccgggca ggttgaagac gttctcgata acgatggccc cggccagaag ctctcccatc
     2101 tggagcccca cgatggtgac caccgggatg agcgcattgc gcagcgcgtg tttgtagacg
     2161 acgactctct ccgcaagccc cttggcccgg gcggttcgga tgaagtcctc ccccagagcc
     2221 tccagcatgc aggagcgggt gagccgggcg agcacggcag ctcgaattag tcccagagac
     2281 agggcaggga ggagaaggga tttgagagcc cccagggggc tttccatcca cgatttaaat
     2341 ccccccgccg aaaaccattg gaggtaaacc gcgaaaaaca ggatgagaag gatcccggcc
     2401 cagaaggcgg ggaccgccag gccgatttgg gaaaagacca tcaccccgta gtctccgggg
     2461 cggttgcggt gaagagcggc ataaaccccc agaggaatgg aaaagactac agcaaagaag
     2521 atggaaagaa gagccagggg aaaagtcacc gccaggcggg acaagatcag ttcgctgatg
     2581 gaaacgtcat aggtgatgga aacacccagg tccccccgga agaccccgct catccaattc
     2641 caatactgca ccggcaggga aaggtccaat cccagcttgt gccgaagagc ctgcaggttt
     2701 tccggagtgg cctgaattcc caggatgatc tgggccgggt ctccaggaat cacctggagg
     2761 acgagaaaga tcactcccga gacaaggaga agggtggcaa gaagaatgag gaggcgcttt
     2821 atgaattggt aggccacggg agtaaacaaa aacgttggag gacgcccttc gggcttgagg
     2881 agcattcgct tcgctcactc gaggatggag gcaaaaaaaa cgcttttgcc tcaagcgaag
     2941 cgctcctcaa gttacgcccc aggcgtgacg gtcctcaggg cccgtagggc cggtcctcaa
     3001 gccccttggg ggcggtctat ttttgaatgt acacctcgct ggcatccacg acgatggtgg
     3061 gatagtcctt ccaccagttc atgacttcct ttttcatcgc cgggagcgcc ggatagatga
     3121 aaaggaagac gttgacgaaa tcgtccgcca acatcctctg ggcctgttcg taggtctttt
     3181 tccgggcctg ctcgttcatc tctccttccg ctttcttgat caggtcctgg aacatgggat
     3241 tgttgtagcg gaaatagtaa ttgggcctgc tgtagatttc gatgtcaaag ggctcggcgt
     3301 ggcccatgac ggtcagatcg tagtcggcat ttttgaagac ccggtcgacc cactggccca
     3361 tgggaatcac ttcgataatc agcttgatat ccactttgga aagctggtcg gcaataatct
     3421 ctcctgacct tcgggcataa gcataaggtt cgggcagttt cagtacggca ccgaatccgg
     3481 tggggtatcc ggcctccgcc aaaagctgct tggccttctc ggggttatag gggtagagcc
     3541 cgctcaggtc cacataataa gggttcgtcg ggtccatgtg actgccgatg ggggttccgt
     3601 aaccagccac cgccccctgg ataaccgcct tacggtcgat tgccagggtg atggcctggc
     3661 ggaccttggg atcgctgaag ggcgtgcggg aatgattcat ggccatgatc acgtcggtgg
     3721 tggtgtttcc cttcagaacc ttgaaacgcc catccttctc cagggcgggg acattttcgg
     3781 gggcgaggtc ataggagatg acatccacat ctcctgcccg gagggccgcc agctgcgcgc
     3841 tggggtcggg gatgaatttg aaggtcacct tgtccaggta cggaaggcct ttcacataat
     3901 aatcggggtt cttggccatg atcacgctat ccccgcgctt ccactcgacg aatttaaaag
     3961 cccccgtccc caccggcgca ctcttcagcc ggtccacgga ttgtttagaa acgatgaccg
     4021 cgtcgcccct agccatgttg aaaaggaagt tggaatcgta ctttttcatc ttgaatttga
     4081 ccgtcgtaga atccaccacc tggatggatt cgatgctttc ataatacatc cggtatgggg
     4141 cggcggtctt ggggtccagg agccgttcta agctgaattt cacatcctct gccgtgcagg
     4201 gggtcccgtc gtggaatttg atcccggaat ggagaacgaa ggtatactcc ttgccgtctt
     4261 tggagacgct gtaacccttg gccaaagccg gggccaccct cccgttccgg tctaccttga
     4321 gcagcccctc cagcagatta taatggacga cccgtttgat cgccgccgat gggttggtcg
     4381 tggggtccag tcctgagggt tctgcggcca gggcgatggt gagcgtcccg ccgggaaccg
     4441 gtctctgagc aatggcttcg gaggaaaagg ctccccaaat aacgaaacag aaaaagatca
     4501 acaagagacc cacccacttt ccttgtcgcg aatgaattgc cattcttaaa attccctcct
     4561 cctatctcgt tgggcataat ataattcagt ttattccttt ccttgctatc tcccatcccg
     4621 ggcgacctgc cggtcccctt acttttctct atgccctatg caatatttct ccccccacga
     4681 tggtcatctc caccgggatg tctttgatct catccgggtc aaccttcctg gggtcctttc
     4741 ccaagacgac catgtctgcc aacttccccg gagttatcga gcccttgagg tcttcttcaa
     4801 aggaggcata ggacgaggct ttggtgtaca gggaaatggc ttcttcaacg gaaatcctct
     4861 gccgatcacc caacagttcc cccatggcgc tcttgcgggt cacacagctc tggatggcca
     4921 ggagcggctc gtagggcccg caggggctgt cagaggcgcc cgatacggca atcccataat
     4981 ccagaaaaga ccggtgggcg aacatcatct ccacccgccg ggccccgtaa tggggaatca
     5041 ttttctcccc gtggtggtaa atgtaggacc cgaaaggagt caccaatatt ctgagctttt
     5101 tgatccgttt taatatttcc gggttcacca ccgtgcagtg ctccagccgg tgtcggtgat
     5161 cctttcgtgg aaactcgtcc agggctttct tgaaaccgtc cagggccatc tcgatcactc
     5221 ggtcgccgtt agcgtggacg caaacctgga aacccgcaga gtgtccctga cggatgagtt
     5281 cgtggagaac ctgttccgat tccagcgcca ggattcctcg atcttcagac cccacgtagg
     5341 gctcggaaag gtaggcggtc cgtccggcga ttcctccatc ggccaggatc tttacccccc
     5401 cgatcttcag ccattcgttc ccgaaaccgg ttttcaagtt caagccccgg agctggggca
     5461 gatactcgat gctgatgagc atgtagaccc gcagtttcaa gtctcctttt ttcctggctt
     5521 cctgataggt ctccagggtc ttcgcggaaa cgagcgcatc gtgcacgctg gtaattcccg
     5581 atgccagata gcggtccgcg gccagcttca tgcccctcag tctatccttc agggaaaagg
     5641 gcggaatgat cggcgggcgg ccggtggttc cctcaaagac gaaggcgaat tcagcctgct
     5701 cataaaggat accattcagc cttccgctgc ccgcgtcgcg cccgtaagcg cctcccttgg
     5761 ggtcgcctgc atcttctcga accccgccga tttccagggc tttgctgttc gccactcccc
     5821 aatggccgct gatctgctgg atcaaaaccg ggtggtctgg agcgacttcg tcaaggtccc
     5881 accggcttaa cgttcttccg gctggggttt tagtgtcgtc gtaccccact ccccggaccc
     5941 actccccggc gggttttcgc tttgcctcca gcagcacggc cttttgaata tcctcgatgg
     6001 tcctgcaacg cttggggctg caatcgatct gcaggagatc ggtcccgtac tgggacaaat
     6061 gctggtgcga gtcgatgaaa cccggcgtta ccgtccgccc ccgcagggcg accctccggg
     6121 tcttgttgcc cgccagggct ttcacttcct catcgctgcc caccttcagg atgcgtccat
     6181 ccttgatcgc cacggcccgg gctgcgggtc ttcgggcgtc cacagtgatg atatttccgc
     6241 cccagagaat catatccgcc gaagcctctt gcggtaatga acaggttcta ctcttctgta
     6301 ccttcatgcg actccttggg aaatagcttt cagcggtcag ctatcagcgt tcagctagaa
     6361 ccaaagaatc tttgtgcttt tgctgactgc tggaagctga acgcagaagg cttcttttat
     6421 tctgccaacg tatccgcgta atccagcact ttgtcgatta ccgccaatcc ttcccggagg
     6481 tcattttcct tgatgcagag aggggggacc acgaagagga aattccaacg caccgtggtg
     6541 tagactcccc cttcgagaag ccttttgttg atctccttgg cgacggcccc gtcgggtccc
     6601 atggcattcc atttcaccag cggttccctg gtctttttgt tcttcaccag ctcaagggcc
     6661 cagaagagcc cgatcccccg ggcatccccc aggcacttat gcctcgcctt catggcttct
     6721 gtctctttcc ggatgatctc gcccatggct atggcgttat ccatcagctt atcttcgcga
     6781 tagactttta acgtagcgat ggccgccccg caagccaggg gatgggcgtt gtaggtcagg
     6841 cccgcccaga gcatctgctt gtccaggact tccatgatct ttcgggagat ggccaccgcc
     6901 cccaggggga catatcctga agtaagccct ttagccatcg tgatcatgtc gggcaccaca
     6961 ttccagttgt cgatggcgaa ccacttgccg catcgtccga acccggccat gacctcgtcg
     7021 gcgatcagca gaaccttgtt ccgagtgcag atttctctta gcctctgcat atagccgtct
     7081 ggagggacga tcaggccgtt gctgcccacc acggactcca cgagcacggc cgccaccgtc
     7141 tcgggagtct catacccaat gacttcctct accgaatcgg cgcagcgcag gttgcaggcc
     7201 gggtaggtca ggccgaaaga gcagcggtaa cagtagggat cgaagacgtg cacgactccg
     7261 ggcatgaccg gctccaccgc aggacgcctc gggtctccgg tcagggcgat ggccccgtag
     7321 gtggcgccgt gataagaacg gtaacgggcg ataatcttcc attttttagt atacatgcgg
     7381 gcgatcttca ccgcattctc gttggcgtcc gccccgccga gggtgaagaa gaatctcttc
     7441 aggtctcccg gggtcacctc cgctaaggcc tttcccagga aggagcggga ttcgtaggcc
     7501 acccccgggg cgacgaagca cagcttctcc gcctggtcct tgatggcctg gaccactttg
     7561 gggtgctggt gaccgatgtt ctggttcatg agctgggaag tcatgtcgat gtaccgcttc
     7621 ccgttctcgt cccacatgtg cacgccctgg gctttggtga tcaccagcgg gtccagggcc
     7681 gactgaacgc tccaggagtg gaccacgtat tccttgtcat accttttgac gtcgctttga
     7741 ctcatgcctt ttctcctctt ggactaagag cttatcctta aataagattc tgttttttaa
     7801 tccccctttg aaaaaggggg acataggggg atttgcttta gatcgctttt gaaaaatccc
     7861 cccaaccccc ctttacaaag gggggaatat tatttgcgga caagctctaa gcgatattga
     7921 aactttcctc ttcccttcac tccagaaaaa tatttttgtt tccattatgt tatatataaa
     7981 atatttcggc aaggcaaaaa gggaagggaa gcgggttcaa gcggctaata ctttacttcc
     8041 accaggaaga gcccctgggg cggggcggtc atgccggcct ggcgccggtc ccgcgcgcgc
     8101 aggacctgcc caaactgttc cggggaccgc tttccctttc ccacctctac cagcgtaccc
     8161 acgatgtttc ggaccatatg cttcaggaag ccgtcggctt ccatggtgaa gtgaagaaaa
     8221 tcggagtcct tcttcgacca tccgcactgg aacacttctc ggaccgggtc ttcggtgtcc
     8281 gcgtccgccc cccggaaaga ggtgaaatcc tgcctcccgg taagaaggcg ggaggcttcc
     8341 tccatagccg ggacatccag ggccccagga acacgccagg aataattccg gaacagggcg
     8401 gagggaactt cgcgatggag aatgacatag cggtaggttt tccgcttggc tgcatactgg
     8461 gcgtgaaact ggaggtcgac ttcttcgacg gactgaatgg cgatgtcggg tggaaggagg
     8521 ctgttcagcc cccgatgaaa cccttcgacc ggaatagtgg aatcggtccg gaagttggcc
     8581 acctgcccca gcgcgtgcac ccccgcatcg gtcctcccgg agccggtcag gcggacgcgc
     8641 tgccgagtca tggtctgaat tttctcttcg atgacttcct ggatggtcag gccgttgggc
     8701 tgaacctgcc acccgtggta attcgtcccg tcatactcga tgaccaactt gatattgcgc
     8761 atgaatgagt ttcgggtttc gagtttcggg tttcgggttt aaactcgaaa ctttaaactc
     8821 gaaacccgaa acttaagatg gtcagggcat ttccgcgatc agcgcgccgc ccattcctcc
     8881 accgacgcag agggtggcca gcccgtaatg ggctttccgt ttcttcatct cgtaaagcag
     8941 cgtaaccagg atgcgggccc cgctggctcc tatgggatgg cccagggcca cggcgccgcc
     9001 attgacattg accttttcta tatcccagcc catggtccga tttacgtaaa ccgcttgtgc
     9061 cgcgaacgct tcgttggctt cgatcaagtc caggtctttc accttgagcc cggctttggc
     9121 cagggctttc tggctggagg gaaccggccc ggttcccata atgtccgggg caaccccggc
     9181 cgtggcgtag gccttgattt tagccagggg ttcgactccc agcgctttgg ctttttccgc
     9241 cgacatgacg acgatggctg cggccccgtc attgattccc gaggcgttgc ccgcggtcac
     9301 cgttccgtct ttcttgaagg ccggcctcag cccggccaag gcttccatcg tggtgttggg
     9361 gcggggatgt tcatccacct ggaaggccac cgggtcccct ttcttctggg gaatcataac
     9421 cggaacgatc tcctctttaa accgcccttc cttgatcgcc cttccggcgt tctgctgact
     9481 ccggaacgcc agggcatcct gttcctgcct ggaaacgcca aatttttcgg ccacgttttc
     9541 cgcagtgatc cccatgtggt agcggttgaa gatgtccaag agcccgtcgt ggatcatccc
     9601 gtcgatcagc gccccgtcat tcatgcggta gccccaccgc gctccggaga gcaggaaagg
     9661 ggccatgctc atggactcga tccccccggc catcacaatg tccgcgtccc ccgccttgat
     9721 cacctgcgcc gccatcatga ctgatttcaa gcccgaggcg cacaccttgt tgatagtggt
     9781 ggcggggatt tccttgggga tccccgaccg gatgagtacc tgccgcgccg ggttctgccc
     9841 ctgacccgcg gtgagcacgt ttcccagaat aacttcttcc acctgttctg gcttcatctt
     9901 cgccctcttg agcgcttcct gaacggcaat ctttcccagc tcaacggccg ggatgtcccg
     9961 caaggcacca ccgaaggttc ccaccgcggt acgaaccgca ctggcaataa ccacgtctct
    10021 catgtttttc ctccattttt tcattcagcg cagagaacat gggaattaac caaaaatcca
    10081 atttgccgga aataagtccc caatagctac aaatcgccaa agactccaac cgactttttt
    10141 tatgcctcgg cgaattctgc actctctgcg gtgaatctgg ttttgttcta tcccttgtca
    10201 gcaatcttgg ccagtttttt cgccagcttc ttcttctgtt cgatgttccc ggcgcgcatg
    10261 tacatcacct tctgggcctg gggggagccg gcgccgtgca tggactccac cagggccgtg
    10321 cccccggtca tgttttcgat gaggcgaaga aggcgaatcc ggtcttcggt gggtatgtct
    10381 ttcaccccct tcaggtactt ctccacgtat ttgctcactt ccgggttctt caggtcatcc
    10441 tcgaatggca gggtggcgat gattccgccg gccacgtcat gagccaggcg cgccacttcg
    10501 taaatgttct tggtcacatt gtacttggtg gtgttggcca gaagggtgtc gggttcccag
    10561 tttccggcct tggtcttgtg gcccatggcc gagcaggcaa tgctgccggt gtagatggtc
    10621 tcggccagat gggtcatctc cgccagcttg tcccgcacgt gagaggcctg ggacgttccc
    10681 tggtaatcgg cagccagtgc ggtggctccc accaggacgt cggtcaaccc ccctttgcat
    10741 cccccgtagt tctgccgatg aagggaagcg aacctttcca ccaggaggcc ggcgaattca
    10801 acctccccgc acatgaagac ccgctcccag gggacgaaga cgtcttccag gaccaccagg
    10861 gcttctcctc ccaccagccc aaattccggg tttcccgcgt ccatcccctg ttccagtttc
    10921 cgctcttcat tggtctggcg cccgaagatc atggttacac ccggggcgtt gaccgggacc
    10981 gcgcaggcga tggcgtagtc ttggtcctcg ggccgcaggg cttgggtggg catgatgagc
    11041 attacgtggg aattcaccgc cccggtctgg tgggccttgg ccccccggat gaccactcct
    11101 tcctcggtct tcttgactac gtgggtgaag aggtccgggt ccgcctgctg gctggggccc
    11161 ttggagcggt cgcccttggg gtcggtcatg gaaccgacca ccatgaaatt attttcctgg
    11221 atgtaggcga cgaacttttt cagccggtca tggtaagagg tcccgtgctt ttcgtcgatc
    11281 tcataggtga cgctgtaaag ggaattcatg gcgtcaaacc ccacgcagcg ctggtagcaa
    11341 ctcccggttt cgtgggagat caggcgaagc atctggactt tttttatgag gtcgtcccgg
    11401 ctctggtgga cgtgggtgaa gcggttgatc ctcttcccag tgaggtggga tgtcgcggtc
    11461 atgaggtctt catgctccgg cttcagcgcc agttcgtagg tcttggacgc ggcgttgata
    11521 tggggaacga acgccgggtg cgcggtcaca tcctccaccc gtttcccccg ataatagacc
    11581 acgtgcttca gctttttcag actctcgatg taatcttctt tagtcttgat catccttaaa
    11641 tcccttccct ggttgctggt tcgcaattct tcgttcctgg ttctcggcct taaccagaaa
    11701 cgagcaactc attttattat ttccccttca ggatattgct cgatatgacc aatctcatga
    11761 tctcggaagt cccctcgtaa atctccgtaa tccgggcgtc ccggaagtgg cgctccacgt
    11821 tgtattcctt tatgtagccg taaccgccgt gaacctgaat ggccttggtg gcgatccggc
    11881 tggccgcctc cgaggcaaag agtttggcca tggccgcctc cttctcgtag cgttgtttat
    11941 ggtctttgag aaaagcggcc cgcagggtca gcaatcgggc ggcatccagg tccgtggcca
    12001 tgtcggcgat catccactgg attgcctgga gtttggagat gggggcgccg aactgcactc
    12061 tcgtcttgga gtattcaacg gccgcctcca aggccgctcg ggcgatgccc aacgcctggg
    12121 aggcgatccc gatccgcccg ccgtccaggg tgttcatggc caccaaaaat ccctggccca
    12181 cttgtccaag gaggttttcc ttgggaatgc ggcaatcttc catgatcagc tccgcggttc
    12241 ccgaagcctt gattcccagc tttttctcca ctgctcccac ggaaaatccc ggggccttgc
    12301 aatccatgat gaaggagctg atccctttgt atcccatttt cttgtcggtc atggccatga
    12361 gcacgcaata atcggccacg tttccgttgg tgatgaagtt cttttttccg ttgatgaccc
    12421 actggtctcc ctggaggacg gccgttgtct tctgggcggc ggcatccgag ccggcctctg
    12481 gttcagtaag gccgaaacag cccagtttct ttcccgaggc gagaggagtc aggaactttt
    12541 gtttctgtgc ttccgtgcca aacgtatgga tcgggtcgca gaccagggaa ttgttcaccg
    12601 acatgatgac ccccacactg gcgcagcccc gggaaatctc ctccatggcc accacgtaag
    12661 aaacgatgtc ggctcctccg ccgccgtagg tgtccggaat ggcaattccc atcaggctca
    12721 gttcggccat tttctgaata atttccgcag ggtgacgctc ggtttggtcc agctcctggg
    12781 cgttgggctt gatttcctgg tcggcaaagt cgcgggccat ggcttgaacc atcttttgtt
    12841 cttccgtgag ttcgaaatcc atccccgact cctttcctcg tctgtcgttt tggaatagca
    12901 gaggtcttaa gatttccaag agttccagga aacgaatgtt tctcagtcgg cgatattgta
    12961 ataacttcgt ccgggaaatg tcaaaggcaa attctttttc tgggtaagag ctcaggggtt
    13021 tcgggattcg gaggtttggg acaaagattc aggtggggtt aaaaaaggaa ccaaatgcgc
    13081 attattctcc attttacggc ttgaataccc aaaatccccc tcctccctct ttgggccttt
    13141 tacaagctct acattttttt tttattttca tatctccccc gaccggcata gataaatgaa
    13201 ttatttattt tttttgacga taaggtacaa gaaagagccc atttgaaaag gccaacgccc
    13261 cgccagacct taaacatttt ccttgacaaa cacaggaaac gaaaggtatt ttttgtaaag
    13321 gctggctagg catggacctt gcataaaatt taataaaaaa ttatgggggt ctgcaatggg
    13381 acacaagatt aggactttga ccgccgcgct gttagttctg agctgcctgg ggatatgggg
    13441 gacggttgcc aagaggaatg cccaggctgg ccaaaccctg gacctttcca atgtgatggc
    13501 ccgggatcaa attcgggcct gcggtttcat tgcccccaaa atgatactct ctttgggatc
    13561 gattgtggga agccaggacg ccagtggcaa cctgagcgaa ggggacttgg tctacataaa
    13621 gctggaaccc ggaaagcagg tgaaggccgg ggatagactg gccatcaccc gggtagtcaa
    13681 ggaaatagag caccccctta cccgggtaaa gctaggctat caggtgagtt ttcccggacg
    13741 ggcggtcatc ctggacggga gagggcaaat cgttcccgca aagatcgaaa aatctttcca
    13801 tcccattcgc tacggggatt tagtcaccgc tctgccttcc tcttccccct cggaggttgc
    13861 ggttcgtatg ccagataagg taaaagggac cgttgtggcc gcggcgcaag aagaggaaaa
    13921 catatccgaa aggggagttg tttttatcga tcgagggtct caagacgggg tgatcatggg
    13981 ggaccttttt accgtctatc aattccccta ctataccgat gaggccctcg aaaaagagcc
    14041 aaagctccct ctgatgaaag tgggggaagg ggtggtcatt tacctggaga aggaaacctc
    14101 aacccttctg cttactcatt cttcacagtc tgtgtatgtc ggagacaccg tcgtcttggg
    14161 gaaggggaag taagctccta agttctcaaa ataaccggca agggaaacag tctgggatca
    14221 tccaggaata gggattgtgg cctgaatcat ccacgatccc ttttttattc ggtttccttc
    14281 ccatggaatc aaaaggactt taccactggg tagcgctctc catggccccc ggagtgggga
    14341 gcatcctttt taagcgtctt accgaggcct tcggcggccc ggagggcgtc tttcaggccc
    14401 gggccaagga cttggaacgg gttgagggga tcgggcccaa ggtcatcgaa gctctgaagc
    14461 ggttccattg gaaggagcag gtggataagg agcttcgctg cctggaagga atcggggctc
    14521 gcctcctgac ctggtcggac gaggaatacc cccctgggct gaaacagatc tacgatcccc
    14581 cgcctttgct ctatgtccgg ggctgcatca aagcccagga tcagagagcg gtggcagtgg
    14641 tgggttcccg atatcccact acttatggga agatgtttgc cgaacgaatc tccctgggac
    14701 tgagccagcg gggagtcacg gtggtcagcg ggttggcccg cggggtcgat tcagacgccc
    14761 accggggggc attggccgcc ggggggcgaa ccatcggggt cttgggttgc ggcatagatc
    14821 tcatttaccc cccggaaaat gaaaagttgt tcggggaagt ggctgggcag ggggcgattc
    14881 tctccgagtt tcccctggga actccgccgg agaaggatca cttccccatt cgcaacagag
    14941 tgattagcgg catctccatg ggtgtggtcg tggtggaggc gacgctgcgc agcgggtcct
    15001 tgattaccgc gcgctttgcc cttgagcagg ggcgcgaggt ttttgccgtc ccgggaaaca
    15061 tagattcggc ccggagcgcg ggaacaaacc ggcttatccg ggaaggagcg aagctggtcg
    15121 cccgggtgga ggatatcttg gaagagattt ctcttcctgc cttgccagtt caggaggaaa
    15181 cgacgccgaa acccaagttg accgaagaag aggcgagggt tttttccgca ttaggacccc
    15241 aggccgcgca tatcgaccaa gtcattgccc aaagcgccct tcccagcgcg cgggtctcgg
    15301 tgatccttct ttccctcgag ctggccgggc atgtcaagca gctcccggga atgcgctttg
    15361 taaaaacgga tttttagcca cggaggtccg aggaccgctc aggcgcaacc aaaatagaaa
    15421 aggcgcgttt taccatgatc caagttctta aagaaaattg tgggaccgtc gcgccaagcg
    15481 cacccagttc cccatttgcg gaccgtgccg cccaaggcgg ggcgctcccc tcgcgagaga
    15541 aaagaacgtg aacccttttc tggaaaagat gtcaggattt caaacgttgc acccggagcg
    15601 tgctgcaaga agataattga cattgaggaa ttatgtatta tgtttatttt tttaggtttc
    15661 tttccccctc cggggttgag aatactttga ggagatgaaa tgtccaagcc gttagtcatc
    15721 gtcgaatcgc cggccaaagc ccggacgata tccaattacc tgaagaatga atatatcatc
    15781 aaagcttcag tgggccatat caaagacctt cccgagagcc gcctgggggt ggatatcgaa
    15841 aagggatttg cccccgaata tcaggtcatc aaggggaagg tcaaagtcct gaaagaaatt
    15901 aaaaccgcct cgcaaaaggc caaagagatt tacatcgcca cagacccgga ccgggagggg
    15961 gaggccattg cctggcacat cgccgaggaa atccatcagc cggcaaagga aatccaccgg
    16021 gtcctcttca atgagattac ggctcgaagc gttcgggagg ctatgctgaa acccgagcgc
    16081 ctgaaccagg acaagtttga ggcccagcag gcccgtcgca tcctggaccg tttggtgggc
    16141 tacctgataa gtcccctcct ctgggacaag gtccgcagag gattaagcgc gggccgggta
    16201 cagaccgtgg ccgtgcgcct catttgcgag cgggaagaag aaatccgtaa attcgtaccc
    16261 caagaatatt ggtctatcga agcctccctg gaggggaaaa accccccgcc tttcaaagcc
    16321 gggctcttcc ggatcgattc ggagaaggcc gagatcaaaa ccggggagca agcccaatcg
    16381 attctgaagg acctgagttc gcttcctttt gtcgtcaagc agatcgaaag aaaagagcgg
    16441 cgcaagaatc ctgcccctcc gtacataacc agccagctcc agcaggaagc ctggcgaaag
    16501 ctacgcttta cggccaagaa gaccatgtcc atcgctcagc ggctttatga aggggtcgaa
    16561 ctcggtcagg aagggcccgt gggtctcatt acttacatgc gcacggactc ggtccggatc
    16621 gccccggaag cgctgaccgc ggttcgccaa ttcatccaag agaaattcgg tgcggattac
    16681 ctgccggaga agcccaactt ctataaaagc cggaaaacgg cccaggaagc ccacgaagcc
    16741 atccgcccca cttccatgca atatgagccc gaaagggtga aatctttcct gaccaaggat
    16801 caactgtctc tgtatgacct catctggaac cggttcgtag cctgccagat gaacccggcg
    16861 gtctacgacc agaccacggt ggacatcgaa gccggaaagt acaccttccg ggcaacggga
    16921 tcggtccttc tcttcccggg gttcatggcc ctttacatcg aggccgtcga agagaacgga
    16981 aatggagaag aaaaggaagg gaaacttccc gaactggccg agggcgagat cctgaagctc
    17041 ctggagctta cccccgagca gcacttcacc cagcccccgc cccggtacag cgaagccacc
    17101 ctaattcggg aactcgagga aaagggaatc ggccgcccct ccacctatgc caccattctc
    17161 gacaacatcc aggaaaggga gtatgtagcc aaagagaagg gccgtctgtt tcccaccgag
    17221 ctgggctgtc tggtcaccga tcttttggtg atgaatttcc aggatatctt cgacgtgcag
    17281 ttcacggccc agatggaaga tgaattggac aaggtggaag aaggggaact ccgctggacg
    17341 caggccctgg aagatttcta ccaccctttt caaaaagacc tggaaaaggc caaggtggag
    17401 atggaagaca tcaaatggaa gggaattgcc accgacctga cttgtgagaa gtgcggcgct
    17461 cccatggtga tcaagctggg acggcatggg cagttcctgg cctgctccaa ctacccggaa
    17521 tgcaagaaca ccaaggagtt ccaacgcaac gaggccggtg aaattcaggt gatcgaggca
    17581 gaacctatgc aagaaagctg cgagaagtgt ggcgccccca tggtgattaa aaccgggcgg
    17641 ttcggaaagt tcctggcttg caccaactac cccaaatgca aaaataccaa gaagatgatg
    17701 gtcaacgggg agggcaagct ggaggtcgcc caagaggaag tgagctccga agtctgcgag
    17761 aattgcgggg ctcccatggt gatcaaagcc gggcggttcg gtaaattcct ggcctgctcc
    17821 aaatatccgg agtgcaagac cacccggagg atcggcgcgg aaggcaagaa gggggaggct
    17881 tccaaagcgg agcccacggg ggaagactgc gagaagtgcg gcgcgcccct ggtctaccgg
    17941 aaggggcgct tcgggccttt catcgcctgt agcaactacc cgcgctgccg ctatacccaa
    18001 aaaatcgcca agggggagaa ggcggtgggg ctcccggcag aagcggcgtc ggcggccaac
    18061 cctgaacaga agaagccgga ggctgccaag gaggagaaaa aggaaggcgc cgagaagaag
    18121 acccggaaga aaaaggccta ggcgggcttt ccagtgccct acaccttgcc ctctgtgttt
    18181 ttcgggtgct catattccgc attccgcact ccgaattccg catttacttg accagccaaa
    18241 ctcccaccac gatcagcgct gtcccgatga ctcgggaaac cgtgaggccc tcttgcagaa
    18301 tgagccagct gagcaggacg gtaatcaaag gatacgttgc cgtgatgggt acgactaaag
    18361 aagctccccc caacctcagg gcatggtaat aagtccacag gcccagggcc cctccgcaga
    18421 ttccgctcag cccaaaataa atagttgaac gccaatccag ggctgccaga ctccccaacc
    18481 gtccgcttcc cacccctacc accaggagaa ttccggtgag gatgaattga cggatcatca
    18541 tgccggttag aggactggtc tttcctaaag ccagcttgtc aaagatggcc gccattcccc
    18601 agaagaaagt cgtcaggaac atgaaaagca tgagtttcat gggagggctc ctgttttttt
    18661 ctcttcaccc attaaggaat aaatttcccc ctgatgcaag cctcaaattg aggataaaaa
    18721 aaggaggtgg ccccgttgga accacctcct tcaattgtct gcagtattat ctccccatgg
    18781 accgtagggg caggtttgag acctgcccct acgccgtttc atggattaga acgccgcttc
    18841 ctggatggcc aaggcgctcc gatttcctcc gaggaccgtc gcagccttgg cctggacttg
    18901 cggcaagatg ttggtcacaa agaactcggt cccatagacc ttgccgctgt aataggcggc
    18961 gtcggcctgt tcttcaatga ccttggcctg ggcttcgggc gtttttgcgc cggctttgtc
    19021 gtaaagggcc tggagttttt ccttggccac cagggactgg tccaggagaa ggtaagccaa
    19081 ttgtacctcc ccaaacatct ccaaaaacgg aacacactga agcatagggt agagcgggtc
    19141 tcccgtggcg gccgtcttgg cgaagtgcat ggccgtgtta accaaggaat ttttggcctg
    19201 ctccagctgg gtcacgaaag accccagcgt cggatgccct ttgtgtttct cgataaactc
    19261 attcatttcc ttcatccagc ccatgaccag ggcgcctttc ttgagggaga gcttccttcc
    19321 caccaagtcc atggcctgga tgccgttggt cccttcgtag atggaggtga tcttcacatc
    19381 ccggcagagt tgctccacgg gatattcgcc gcagtaccca tacccgccat gaatctggat
    19441 ggcccattca gccatgcgga aggccatgtc ggtggaatag gctttcacca ccgggatcag
    19501 cagatcgatg gtgttttcgc agatttcctt ctccttcggg tccgtggcca ccttggccag
    19561 gtctgcgtag taggccgtct tgtagatgag cgcccgcagc ccttcggcat acgctttcat
    19621 ggtgatgagc attcgccgca catcgggatg gtttatgatc gttactcggg gggcattggg
    19681 gtctttcatc ttccggatat ccaccccctg gactcgttcc ttggcgtatt tgagggcata
    19741 taggtaagcc aggtttccca gagcgaatcc ttgcaagccc acgcccaggc gggcttcgtt
    19801 catcatctgg aacatgagct gaatcccctg cccttcctgg ccgaggatgt agccatggca
    19861 tttcccgtca tccccgaaat tcagggtgca ggtcgccgag cctttcaggc ccattttgtg
    19921 ctcgatgccg ccgcaattga catcgttggg ctcccccagg gagccgtcgg cattaattcg
    19981 gaccttgggc acgacgaaga ggctcaatcc tttgatcccc ggaggggcgt tttccgcccg
    20041 ggccaggacg gcgtggatga tgttgggcgt caggttgtgg tcgccggcgg tgatgaagat
    20101 cttcgttccc tggattaaat aatgatctcc caccttcttg gccgcgcatt tcacgtcccc
    20161 cactgcgctt ccggcctggg gttcggtcag gcacatggtc ccggtccact ccccggcgta
    20221 catcttttca agataggtct ttttctgttc gggggtcccg aagctctcga tcagattggc
    20281 gcagccccgg gttagcccgg ggtaggtggt gaaagagcag cacgctccga cgaacatttc
    20341 tgcggcggcg agagccactg aatctggcag gccctgtcct cctgcttccg cgtctaccga
    20401 tgcggcgatc caaccgccct cgcagtattt cttgaatgca tcatggaagg cttccggaac
    20461 cttgactttc cctttttcgt acgtgcaccc ttccttgtcc gcaaaggcat tcaggggggc
    20521 gatgacttcc gcggaaagct gggcggcctg gtccaggacc atgtcaaagg tctcccggga
    20581 gtagtcctta aatttatcga attgacacag cctctcgatt ccaagccatt cgtaaaggat
    20641 gaatttcatg tctctcatgc tgatcgggaa agctccattg ctcatggcca cctccttgtg
    20701 cgggttgatc tgcctccaat cgggtgcttc tgcccgcatt ctaaaaaaaa ttgcaggcgg
    20761 ttgtcaagaa aattcgcatt caaactattc gctcccgtaa taaattcttc attggcacgg
    20821 ccagtaaata aaattaaata attataggaa gttggagaat aaaattcatt ttctccttgt
    20881 cagatggctg gccaaaagat tataaatgac tccgatatgg tcgcgtaaaa aatacaaaaa
    20941 gggtcattgc tggcgaagcg aaccaatctt atagtctggc tcttggattc catgagattg
    21001 cttcgtcgct gcggtccccg caatgacgcg aactaagcgt ttcataaaat ctttatggaa
    21061 taggttcgtc attcacgcga aagcgggaat ccagaaagaa atcctggatt ccgggtcaag
    21121 cccggaatga cggatgctaa ggttctccga aagtgatatc aaggtacagc atgacaatcc
    21181 tcatgagatg caatcctgca aacggttctc attatgcgtc agcatgaatt tatgcacctg
    21241 atgggtcgac tctggacttt ttactggttc aacaataatg attggacgga taaagggaat
    21301 taggatggag tttgaatact ggctcgatca acatttaacg aaatctgagc gccgaatctt
    21361 gagccgtttg accactccga gtaaaatcca ggcttttcta gatgacctgg aatattcaac
    21421 ggaagaaatt tatcgttgcc cccttcgagt tctgcgggag cgggtggccc actgctacga
    21481 cggtgccctc tttgcagcgg caatgctccg ccgcatcggg tacctcccct tcatcctgga
    21541 aatgcttccc aatagccgcg acgacgatca tctcttggcc cttttcaaga aggaaatgca
    21601 ttggggcgct gtgggtaagt ccaactttgt gggacttcga tttcgggaac cggtctatcg
    21661 gacggtacgc gagttggtca tgtcctactt tgaacaattc tacaacctgg agagggaaaa
    21721 aactctgcgt gcatacacgg tacccttgaa tttgcagtcc ttcgaccggc atggctggat
    21781 gattaaggac gagcccctgt attggattgc cgagaaactg gaccggaccc gaagagtccg
    21841 ggtactcacc ccgaagatgg tcaggaacct ttcgctcttg gacccccgag cctaccaggc
    21901 gggccttatg ggagccgacc aaaaaggact ctttcatccc cgtcgaaagg gttaactccc
    21961 tgactccctc cagccaggac agagaagagc gggtcgtagg ataggaatga aatttagcag
    22021 aggctgtggc ctgggaaaaa ggagggtccc taggcccctt taagggtgtg tggatgaggg
    22081 agggccaaag gggccggtgg acttcattct ttcagggatt attcaaaaag gcgtcatccg
    22141 gatattcaat ttttataatt tcgctgagtc taaattcctg ggtcctggca aatcgttagc
    22201 acaaataggc cccgaaccct aaagctgtca atccccacca gatccaataa tccatctctc
    22261 ggcccttctg aggcgtaatt tggatgtggg cattctttga taaagctgaa gaggagaaag
    22321 aaaatttcag gggttggctt gttggtgaat tgcgagaatg cagttaggaa gcctcctaac
    22381 cgttccgatg gtttcttgtt ttatttagtt caccaagtcg tcaccttgag atattccaag
    22441 aggatgagga ttattggcag aaatggcgca ccccaattca atagatgaag ggggcttctg
    22501 cgtgaaatat gctctataag ggcaccaagg gaatggaaaa gagccacatt ataatcaaaa
    22561 gcaaagctcc tattaccatt gcttgcaaaa ttatttgcat tttcccccct ttgatctagt
    22621 gtcgcgtctc agaaataagc tgcataattc tacccccgag cagggagcgg ggcgaagaac
    22681 cgccgcgctt gccaaaataa ggcgctcgtt cctctttgaa aaagttgatt tttactgaag
    22741 gtttcggaaa ttgtatattt ttgcgctcct agcctaattc ccggaggctg gcagcgtcgc
    22801 tttgcggata ccaggcaacc gcctggagac aggccatttc ccggtcgatt ttggcggcgc
    22861 gcatcgtttc tccacccccc ctcctg
//
LOCUS       DASPRR010000526         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2378860, whole genome shotgun sequence.
ACCESSION   DASPRR010000526 DASPRR010000000
VERSION     DASPRR010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378860"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VEL68_19705"
     CDS             <1..471
                     /locus_tag="VEL68_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ38261.1"
                     /translation="RVMNKWNEPEEDLVYQGRIKVPYTWSVGEVGSRFFIELRDNKKI
                     FGKRCPACKKVLVPARKVCGKCSQQTEEWVEVANQGTVQTFTVVRYPSDVQPLNPPFG
                     YGIIKLDGADTGMAHLVYGSDPAKWKIGMKVKAVFKERREGNILDIAYFQPLVP"
     gene            536..1675
                     /locus_tag="VEL68_19710"
     CDS             536..1675
                     /locus_tag="VEL68_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ38262.1"
                     /translation="MDKVGIVGVGQTKYERQKKGDSFADMVFEATTSALQDAQMTIQE
                     IENVITVSNDFWDGRTISSMAVMDACGAYEKNVSTVEGDGTFGVLYGMMRILSGSFST
                     TLVVAHCKGSESVSSLITNGMFDPIYQRALGLDAITSSALQARAYMNKYGISEEECAR
                     VSVKNHGNAKNNPYAQLPMNISVKDVLASRMLADPIKLLDASPISDGAAAVILAREGR
                     AKRIARKPVWIKGIGHCADAYFLGDRDLAECPSLETAARTAYGMAGITSPLKEIDVAE
                     IYDAFSYMELLWSEGLGFCGRGEGGKFIQSGKSQMKGELPINPSGGVLSAHPVLVAGL
                     ARVSECVLQLREDAGARQVEGAKTAVAHGINGPCGQSHCVIVLGK"
     gene            1805..2962
                     /locus_tag="VEL68_19715"
     CDS             1805..2962
                     /locus_tag="VEL68_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ38263.1"
                     /translation="MGKRVAVVGSGQTYHKSARLDVNGIELIHEAVTRALEDTQLTLK
                     DIDAVIIGNMDHFEGINYVDTWSVDGSGAYLKPIFKVTTGGTTGTTVAMAGYYTAASG
                     LFDTVLAIGWEKNSESDTTGAIITAFDPIWDRPAFAGAVAGLAVEASVYMHQYGITEK
                     DAARVSVRDRRHATKNPHAHLRGEITIEDVMKSPMLSYPVKLLDMCPRTDGACAVIFA
                     TEGRAEKITDKPAWVWATASRHNYVYIGDWEPTAGLKTLESASREVYQKVGIRDPLKE
                     IDVMELYLPYSFAGLSWIESLGLCKKGGAPRLLWDGVTDMGGELPINLSGGVISCNPI
                     GATGLIRVAEAAMQVQGKAGQHQAPDVKVAVATGFGGCFWSDIMILGKEKK"
     gene            3066..3533
                     /locus_tag="VEL68_19720"
     CDS             3066..3533
                     /locus_tag="VEL68_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ38264.1"
                     /translation="MEKKDFLTISSGEQAQPFNWSVGRHGSKFLTALRDHKKILGIKC
                     PSCQRVFVPPRRVCGRCFVEMSDFVELPPRGKLTAFTILMFSFVDPDTGKERPVPYGY
                     GYVQFDGSDNTFPHFLEETDWKKIKIGQRVEAVFEEKRRGHLLDIKHFRLVKE"
BASE COUNT          924 a          972 c         1108 g          825 t
ORIGIN      
        1 agggttatga acaagtggaa tgaaccagag gaagacttgg tctatcaggg aaggatcaag
       61 gttccctaca cttggtcggt gggcgaagtg ggctccaggt tcttcataga acttcgggac
      121 aacaaaaaaa tcttcggaaa acgctgcccg gcctgcaaaa aagttctggt cccggcgcgg
      181 aaagtttgcg gcaagtgctc ccagcagacg gaggaatggg tagaagtggc gaaccaaggt
      241 acggtacaga ccttcaccgt ggtccgctac ccctccgatg tccagcccct gaatcccccc
      301 ttcggttacg gaatcatcaa gctggatggg gcggacaccg gaatggccca tcttgtctat
      361 ggatcagacc cggcgaagtg gaaaatcggg atgaaggtaa aggcggtatt caaggagaga
      421 agggagggaa acatattgga cattgcttat tttcagcctc tagtccctta atttgtttcg
      481 gattttgata ttcggatttc gaatttaaag cttcagtcag cttcaggggg atcatatgga
      541 taaagttggt atcgtgggcg tgggtcagac caaatacgag cgtcagaaaa agggagacag
      601 cttcgccgac atggtcttcg aagcgaccac cagcgctttg caggacgctc aaatgaccat
      661 ccaggagatt gaaaacgtca tcaccgtttc caatgacttc tgggacggcc ggacgatctc
      721 cagcatggcg gtcatggatg cctgcggggc ctatgaaaag aacgtttcca ctgtggaagg
      781 ggacgggacc ttcggagtcc tgtacggaat gatgcggatt ctttccggct ctttcagcac
      841 aactctggtt gtagcccatt gcaaagggtc tgaaagcgtc tccagcctca tcaccaacgg
      901 catgtttgac ccgatctatc aaagggccct gggtctggat gccatcacat cttcggccct
      961 gcaggctagg gcttacatga acaagtacgg gatcagcgag gaagagtgcg cccgggtctc
     1021 ggtgaaaaac cacgggaatg ccaaaaacaa cccctatgcc caacttccca tgaacatctc
     1081 ggtgaaagac gttctggctt cccgcatgct cgctgatccc atcaaactcc tggacgcctc
     1141 gccgatttcc gacggggcgg cggcagtcat cctggcccgc gagggcagag cgaaaaggat
     1201 tgcccggaaa ccggtgtgga ttaagggcat cgggcactgt gccgatgcct atttcctcgg
     1261 agaccgagac ctggccgaat gcccttccct ggaaaccgcg gccaggaccg cgtatgggat
     1321 ggctgggatc accagtcccc tgaaagaaat cgatgtggcg gaaatttatg acgccttcag
     1381 ttatatggaa ctcctctgga gcgagggact gggattctgc ggcagagggg aaggggggaa
     1441 gttcatccaa agcgggaaaa gccagatgaa aggggagctt cccattaacc cctccggggg
     1501 agttctctcc gcccatccgg ttttagttgc cggacttgcc cgcgtttccg aatgtgttct
     1561 tcaactcaga gaagatgctg gggctcgcca agtggaaggg gcgaagacgg ctgtcgccca
     1621 tgggatcaac ggtccctgcg gacaaagtca ttgtgtgatt gtgttaggaa aataagctat
     1681 cagcgatcag ctgtcagctt aaagcatttg ttctttccgc tctcccgata aaggaaaagg
     1741 attagggtga gggggggagc tgaatgctga tagctgatta ctgaaagctg cctttggagg
     1801 atttatgggc aaacgagtcg ccgtcgttgg gtccggtcaa acttatcata agagcgcccg
     1861 cctggatgtg aacggtatcg aattaatcca tgaagccgtc acccgggctt tggaagacac
     1921 ccagcttacc ctgaaagaca tcgacgcggt catcatcgga aacatggacc actttgaagg
     1981 gatcaactac gtggatacat ggtccgtgga tggctcgggc gcctacctga agcccatttt
     2041 taaagtaacc accggaggga ccaccggaac gacggtggcc atggccggct attatacagc
     2101 ggcctccggg cttttcgata cggtgctggc cattggctgg gaaaagaatt ccgagtcgga
     2161 caccacgggg gccatcatca ccgccttcga tcccatctgg gaccggcccg cttttgccgg
     2221 ggccgttgcc gggctggcgg tcgaggcttc ggtatatatg catcaatacg gtataaccga
     2281 gaaggatgcg gcccgcgtat cggtccgtga ccggagacac gccaccaaga atcctcacgc
     2341 ccacctgcgc ggagaaatca ccatcgaaga cgtcatgaag tcccccatgc tctcttatcc
     2401 cgtcaagctt ctggatatgt gcccgagaac ggacggggcc tgcgcagtta tttttgccac
     2461 cgaagggcgg gcggagaaga tcacggataa accggcatgg gtttgggcca ctgcctcccg
     2521 gcataactat gtttacatcg gcgactggga accgacggcc ggcctgaaaa ccctggagtc
     2581 ggcctccagg gaggtatatc agaaggtggg catccgggat cccttgaagg aaatcgatgt
     2641 gatggagctg tatctacctt actcttttgc cggcttaagc tggatcgaat cgctgggcct
     2701 gtgcaaaaaa gggggagccc cacgcctcct ctgggacggt gtgaccgaca tgggcggtga
     2761 gctgcccatc aatctctcgg gcggagtcat ttcctgcaat cccatcggag cgacggggct
     2821 gatccgagtg gccgaggcgg ccatgcaggt acagggcaag gccggacagc accaagcccc
     2881 ggatgtaaaa gtagccgtgg ccacaggttt tggcggctgc ttctggtcgg atattatgat
     2941 tttgggaaaa gaaaaaaaat agctgtcagc ggccagctat ctgctaaaaa ccgttcgcta
     3001 tttccctctc cccctgggca gaggggaggg ggagggcggc tgatcgctga attaaggaga
     3061 ttcaaatgga aaagaaggat ttcctaacca tttcttcagg agagcaggcc cagcctttca
     3121 actggtccgt aggtcgccat gggagcaagt tcctgactgc actgcgagat cacaagaaaa
     3181 tcctggggat caagtgtcct tcctgccaaa gggttttcgt tcctccccgg agggtctgcg
     3241 gaaggtgttt cgtagaaatg tccgactttg tggagctgcc ccccagagga aaacttaccg
     3301 ccttcaccat cctaatgttc tccttcgtgg acccggacac cggcaaagag cgtcccgtgc
     3361 cttacggata tggctacgtc cagttcgacg ggtccgacaa caccttcccc cactttctgg
     3421 aagagaccga ctggaagaag attaaaatcg gccagagagt ggaggcggtc ttcgaggaaa
     3481 aaagacgagg ccacttatta gacatcaaac acttcaggtt ggtaaaagaa tagatgccaa
     3541 gtaatacggg aggatcataa cccggaagat ggataaaaat tgctccattc ctgataattg
     3601 taattaagcc ttcataaacc aatataacat tcaggattga tttcctccgg tatttcaatc
     3661 gttgacagtc tctggcaaga gatgaagatt ttaatgggta ttggagggca gaggttttga
     3721 tatcccgtcc atatccttaa tcgggtgatc tcttctcaag gtgcggaggt tggcacgaag
     3781 tcgcaactct tggctggtgg gagcctcccc cgataggttg tgccccctt
//
LOCUS       DASPRR010000527         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_128963, whole genome shotgun sequence.
ACCESSION   DASPRR010000527 DASPRR010000000
VERSION     DASPRR010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128963"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VEL68_19725"
     CDS             complement(<1..1125)
                     /locus_tag="VEL68_19725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXZ38265.1"
                     /translation="MSNTVREKIAEMKDWLIETRRIIHMNPELGFEEVETSKLVAGYL
                     GKFGLQVKTGMAKTGVIGLLKGGKPGKTVALRADMDALPMEEANQVPYRSKVKGKMHA
                     CGHDAHVTILLGVARLFSSMGNQVQGNIKWVFQPAEEGGGGGRVLTEEGVLENPKVDA
                     IFGAHLFPDLKIGKVGIHEREGLAATDRFVIRMIGRGAHGAYPHLSKDPILAAGHLIT
                     QVHSIVSRSINPLDSAVITIGKVSGGTAFNIIPDEAEILGTVRSLNQKVREDLKSRME
                     QVTQGVAKSFGVDFHFDFQYGYPALVNDPEMSRLVAAACGKGIGKENVEFIRPSMGGE
                     DFAYYLEKVPGSFFRLGCRNEAKGIVNPFHSSRFDIDEDVL"
     gene            1245..1997
                     /gene="mtgA"
                     /locus_tag="VEL68_19730"
     CDS             1245..1997
                     /gene="mtgA"
                     /locus_tag="VEL68_19730"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HXZ38266.1"
                     /translation="MGKGPLLKICMLFLLLPALFGFAYLVLMPDISQLKKKNPPKTAF
                     MEYREKEWKEKGRRGEIRQVWVPLSQISPFLVKAVLIAEDDKFWSHEGFDYEGIMKAV
                     ERDLQAGRLKFGGSTITQQLARNLYLSPGKSFWRKVREALITWRMERVLSKKRILELY
                     LNAVEWGDGIFGAEAASRHYFGKPASELSPRDAARLAVVLPSPRKLNPAGSQRYVESQ
                     AEVIYDIMQKRGTLPPEFETGADEPGRESFSR"
     gene            2213..2812
                     /locus_tag="VEL68_19735"
     CDS             2213..2812
                     /locus_tag="VEL68_19735"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXZ38267.1"
                     /translation="MGEPIAMERVALQILKKTVDLALSGAETEEPGLFLSSPLIFSKP
                     LVSHDPSSFPEPSELEVESKASNYLPIVQDFESIIQEAGQRYRVDPSLIRAVIQAESG
                     GNPLAVSRAGARGLMQLMPETAAELGVTNPFDPTQNIMGGTSYLSRLLDRYRGDVKLA
                     LAAYNWGMGNLERHPEAMPRETKNFIAAVEKNQRNPIKA"
     gene            complement(3013..>3621)
                     /locus_tag="VEL68_19740"
     CDS             complement(3013..>3621)
                     /locus_tag="VEL68_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HXZ38268.1"
                     /translation="SKDGKATATSLGKPFPSRKVKIVDPETLKEVNTGQAGELIVSSP
                     YILPYLNNEKETEKSFVEVDEKKWYRMGDFVRMDGNGELHFVERTADIIKYSGYRVSA
                     SEIEAVLQNHSSVIGACVVGIPDPMVGERIKATVVLKEDARGVGAEDLIAFCKERLAP
                     YKVPQYIDFRDMLPKSKVGKLLRREMREEERRKIEKEFSEKG"
BASE COUNT          837 a         1055 c          845 g          884 t
ORIGIN      
        1 aagcacgtct tcatcgatgt cgaaacggct gctatggaag ggattgacga tcccctttgc
       61 ttcgttgcgg catcccagcc ggaagaagga cccaggcacc ttctccaggt agtaagcgaa
      121 atcctcccct cccatggagg ggcggatgaa ctccacgttc tccttgccga tcccttttcc
      181 acaggccgcc gccaccaggc gcgacatttc tgggtcattc accagcgcgg ggtatccgta
      241 ttgaaaatcg aagtggaaat ccaccccgaa ggacttggcc acgccttggg tcacctgctc
      301 catcctggac tttaggtctt ccctgacctt ttgattcaaa gaacgcactg ttcccaggat
      361 ctccgcttca tcggggatga tgttgaaggc cgtccccccg cttaccttgc cgatggtgat
      421 gaccgcgctg tccaaaggat tgatgctccg gctgacgatg gagtggacct gggtgatcag
      481 atgccctgcc gccaaaatgg ggtccttgga aagatgggga taggccccgt gggctcccct
      541 gccgatcatt ctaatgacaa agcggtccgt ggccgcaagc ccttcccttt catgaattcc
      601 cacttttcca atcttcaggt ccggaaatag atgggcgccg aagatcgcat ccactttggg
      661 attttccaat accccctctt ccgtcaagac ccgccctccc ccgccccctt cttcggcggg
      721 ctggaagacc catttgatat ttccctgaac ctgattgccc atggatgaaa acagccgggc
      781 cactcccaaa aggatggtca cgtgggcatc gtggccgcag gcgtgcatct ttcctttcac
      841 tttcgaacga taaggaacct gattggcctc ttccatgggc agggcgtcca tatccgcccg
      901 caaagccacg gtcttgcccg gcttgccccc tttcagcagg ccgatgaccc cggtcttggc
      961 catcccggtc ttcacctgga gcccgaattt cccgaggtac ccggcaacca acttggaggt
     1021 ctccacctcc tcgaacccca gctcgggatt catgtgaatg attcgacgag tttcgatcag
     1081 ccagtctttc atttccgcga ttttctctct caccgtatta ctcataacca ccccttatcc
     1141 atggttttat aaattccgtt ttctccgcca aaacttttcc tttttattag gatatgttaa
     1201 aaaaaagaaa ctgtcatcca cctttttgaa aagaagacct gactatgggc aaaggaccct
     1261 tattgaaaat ctgcatgctc ttcctgctgc tcccagccct ctttggcttc gcctatctgg
     1321 tcctcatgcc ggatatttcc cagctgaaga agaagaatcc tcccaagacc gccttcatgg
     1381 aataccggga gaaagaatgg aaggagaagg gccggagagg tgaaatacgc caggtgtggg
     1441 tccccctctc ccagatatcc cccttcttgg tcaaggccgt cttgatcgcc gaggacgaca
     1501 agttctggag ccacgagggg tttgattatg aagggatcat gaaggccgtc gaaagagatt
     1561 tgcaggctgg cagattgaag tttggaggca gcaccatcac tcagcagctg gccaggaatc
     1621 tttacctctc ccccggcaag agtttttggc gcaaagtccg ggaagccctg attacctgga
     1681 gaatggagcg ggtcctttcc aaaaagcgga tcctggagct gtatttgaat gccgtcgaat
     1741 ggggcgacgg gattttcggc gccgaggccg cctcccgcca ttactttgga aagcccgcct
     1801 ccgagcttag tccccgggac gcggcccgcc tggccgtggt cctccccagc ccgagaaaat
     1861 taaacccagc gggaagtcag cgatacgtgg aaagccaggc ggaggtgatc tacgacatca
     1921 tgcaaaaacg ggggacctta ccgccggaat tcgaaaccgg ggccgatgaa ccgggaagag
     1981 aatcattctc caggtaggcc ctccccttcc ttacagggga atccctccgg aattccactc
     2041 ctttgaatcc tcttcatttt tcactccaat cccatggcat ggaccttgct tttttaggta
     2101 tacgatgatc ggcaaaattg atccgcaaga tttcctcggc cgtaacccgt tttctacccc
     2161 gacaaatcaa aaacgggatc gagctgattt tctttctttc cttgaagaga aaatgggcga
     2221 gcccatcgcc atggaaaggg ttgccctgca aatcttgaag aaaaccgttg acttagctct
     2281 ctcgggggca gagaccgaag agcccggtct tttcctttct tcacccctga tcttctcaaa
     2341 gcctttggtc tcccatgatc cttcctcttt ccctgaaccc tcggaacttg aggttgaatc
     2401 aaaagcgtca aattatttac cgatagtcca ggattttgaa tcgattatcc aagaggccgg
     2461 acaaaggtac agggtcgacc cctcgttgat tcgggcggtc atacaggccg agagcggggg
     2521 aaacccgctg gccgtctcca gagcgggagc tcgggggttg atgcaactaa tgcccgaaac
     2581 cgcagcagaa ttgggagtga ccaacccttt tgacccgact cagaacatca tgggcgggac
     2641 gagttatctt agcagactct tggaccgcta ccggggagac gtgaagctgg ccctggcagc
     2701 ctacaactgg ggaatgggaa acctggaaag gcaccccgaa gccatgcccc gggaaacgaa
     2761 gaacttcatc gccgccgtcg aaaagaacca gcggaaccct atcaaagctt aaagaccgta
     2821 tctccaagag cccgcaaaga ccccaggaaa ataaaaccct gcaattccaa atcttaaccc
     2881 aaatcctttt tgtagacccc ccctctttct ttttcatcta atcacagcta cttttaggca
     2941 ctcttaacgg aggagtttag gctcgattca tgtttcgggc cgatgaaatg atgtctgtga
     3001 attccacaaa ttttacccct tttcagaaaa ttctttctct attttccggc gttcttcttc
     3061 tctcatttcc cgcctcagca gcttcccaac tttagattta ggaagcatat ctctaaaatc
     3121 aatatactgg ggaaccttgt agggggctag cctttccttg caaaaggcaa taaggtcctc
     3181 tgcgcccact ccccgggcat cctcttttaa cactacagtg gcttttatgc gctccccaac
     3241 catcgggtcc ggaatcccca caacacaagc cccaatgacc gaagaatgat tttgcagcac
     3301 cgcttcaatc tccgatgcgg aaactcgata gccgctatat tttatgatat ccgctgtcct
     3361 ctctacaaaa tgcaattccc catttccatc catccggaca aagtccccca ttctatacca
     3421 tttcttctca tcaacttcca cgaacgattt ttccgtttcc ttctcattat ttaaataggg
     3481 aaggatataa ggggaggaga cgatcaattc gccggcttgg cccgtattca cctcttttag
     3541 agtttccggg tcaacaattt taacttttct tgaagggaac ggcttcccta agcttgttgc
     3601 cgttgccttg ccatctttag a
//
LOCUS       DASPRR010000528         6301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_779555, whole genome shotgun sequence.
ACCESSION   DASPRR010000528 DASPRR010000000
VERSION     DASPRR010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779555"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VEL68_19745"
     CDS             <1..382
                     /locus_tag="VEL68_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXZ38269.1"
                     /translation="YGGFADQRETLAAIRWAFEKQGYLMDTHTAVGYSVLQEYREETG
                     DGTPALLASTASPFKFNQAVLEALGRDTSGKDEFTLLEELSAISGQPVPVKLAELKSL
                     LELHGGVCEREGMAQALFQFLKMT"
     gene            505..1602
                     /locus_tag="VEL68_19750"
     CDS             505..1602
                     /locus_tag="VEL68_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXZ38270.1"
                     /translation="MNAQAMVLEKFKHPLTRKNFPLPKLREGDILVKIEVAGVCGSDV
                     HMWEGRDPRTQLPMILGHEGAGEIAEMKGEKRDVNGVPLKPGDKVLWSRGVTCGHCYF
                     CKVKMEPSLCPHRFVHGIHTSCADPPYLTGNYAQYLLLDQRSDFFRVEKNLDLATLVP
                     ASCSGATTAHAFDLSGMESGDSVLVQGVGPLGIFAVAFARSWGASQVIVIGSTEERLK
                     MCQTFGASLLLNRHRLSPEERRKAILEATGGRGVDVAFEMAGEPEALKECILSLRNGG
                     ACMSAGFGEPHGKMEVDCFQDIGRKNLRLQGIWVSETRHTHMALQLVLSRLRDFAGLV
                     THRFPLNKANEALLAMRSREAVKAVLLPFSP"
     gene            1886..3043
                     /locus_tag="VEL68_19755"
     CDS             1886..3043
                     /locus_tag="VEL68_19755"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ38271.1"
                     /translation="MKLSPAVRERITRLLKGEADTVPILAQINEHVFKLCGGDMREAY
                     TNAEKFVEMNLAVFEYYQLDMPGFYYDIYNIEAEALGQRLNWEADRMPDIDRQNPLIR
                     EHSNLDRLRPPDFKKSGRMPYVLEVMRRCYDLGLPVRVRFCSPFSLAVNVRGIENLLL
                     DILTTPLFAHRLLAFLTDEVLIPWVQAQREATGQPKVSGNGADAAASPPIVNVEILEE
                     FVMPYVARMNQRIGNVTSMGYWGYSYLYKHPEKFYRMLGLMASVSPGALMCLDPDVAR
                     TGPEPYAEFARGKKMSLMLGLDTLLLQDGPLSKIVEQCRRYVLAGAKAERLVIFFNDV
                     SIHTPPQNVHTAIAAVRHFGKLPIKDRPLESFRHSEVESFESFLKKYRQRT"
     gene            3283..4452
                     /locus_tag="VEL68_19760"
     CDS             3283..4452
                     /locus_tag="VEL68_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXZ38272.1"
                     /translation="MAVQRGRHFLQIPGPTNVPDRVLRALSQPTIDHRGPEFSRLTKE
                     VLEGLQKLIKTSGKVIVYPASGTGSWEAALVNTLSPGDRVLMFETGQFATLWKNVAVK
                     LGLQADFIPGDWRHGADPAQIEAKLAEDPQHSIKAVAVVHSETSTGAATRIAEVRKSL
                     DRAKHPALLLVDTVSSLGVMEYRQDEWGVDVTVSCSQKGLMLPPGMGINAVSEKALVA
                     YKSARLPKSYWDWQAMIGPNQTGFFPYTPPTNLLFGLLEALQMIQEEGLEKAIARHAR
                     LGEATRRAVRAWGLEINCLNPLEHTNGLTAVRVPEGHDADALRKKILEKFNMSLGNGL
                     GKVQGKLFRIGHMGDFNDLMLAGTLSGVEMGLALAGVPFKKGGVQAAIDYLAGNE"
     gene            4445..5608
                     /locus_tag="VEL68_19765"
     CDS             4445..5608
                     /locus_tag="VEL68_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38273.1"
                     /translation="MSEPFVGAVREPLLQPDGRSTDPMKILYYLTAHGYGHAVRTSAI
                     CNELSGNLRLIFRTTIPENFFHEEVRKPFEYLPGKFDCGCVQIDSVTVDKKETLQAYM
                     ALAEQNAVCLEEEVKFCREQRIDGIVSDITPLAFEVARKAGLPSVAVANFTWFDIYGP
                     YVREYPSFAPFLEKIRQQYQMADLLLELMPSMEMPYFGNRVRIPPVAKQGRNRSGTLK
                     EHLGLEKDLRIALIYVGELGMEEIRWGELGKFRGWHFLGIYPLPGASANYHLMDKKTF
                     SYEDLVASAELMVTKIGYGVVSQALINGTPLIYLPREDFAEFPVLEKAIQEWGHGYRL
                     SKEDYYAVKWERVLEEVSRRERPKPVPSEGGRICARQIENLIRRSNPGKFPGK"
     gene            5613..>6301
                     /locus_tag="VEL68_19770"
     CDS             5613..>6301
                     /locus_tag="VEL68_19770"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXZ38274.1"
                     /translation="MQFGKKTAAWMVISLLLGWSWDCAGMQPADSIGDFFTGEVLRYE
                     VGFWLIDPVGGGVADFRNLGNGRYMVYHVGKAEGFVGWLTSYRREIYRSTMGTINNGK
                     RLIPLRFEEYSVIGEWFRKKTTVYDYQAHKVFMELEKVGETKSREEIELPLGGLYDDP
                     VTAFYNLRFGVYGKMEQGKEFLLNTLPSKRPEIIRIKVASREETERRRAEEKIKTGKD
                     LLIQILIDREM"
BASE COUNT         1415 a         1706 c         1879 g         1301 t
ORIGIN      
        1 ctatggcggc tttgccgacc aaagggaaac gctcgcggcg atccggtggg ccttcgagaa
       61 gcaggggtat ttgatggata cccacacggc cgtgggatac agcgttctgc aggagtaccg
      121 ggaagagacg ggagacggca ctccggccct gttggcctcc acggccagcc ccttcaagtt
      181 caaccaggcg gtgctggagg ctctgggccg ggacacttcg ggaaaggacg agttcaccct
      241 gctggaggag ctttcggcca tcagcggcca gccggtcccg gtcaagctgg ccgagctgaa
      301 gtctctgctc gagcttcacg gcggggtgtg cgagagagaa gggatggccc aggccctctt
      361 ccagtttctt aagatgacct gacccggatg gtgggcttgt agggtcggcc ctatgtggcc
      421 gcccgcgcga tgggcaacta tgggcaacta ctgagggttg cccctacgct cttaaccgtt
      481 tggcttgtgg atagcgatgg aacgatgaac gctcaggcaa tggtcctgga aaaattcaag
      541 caccccctga cccggaagaa cttccccctg cccaagctcc gggaggggga tatcctggtc
      601 aaaatcgaag tggccggggt ttgcggctca gacgtccaca tgtgggaagg gagagacccg
      661 cgcacccagc ttcccatgat tctggggcac gaaggggcag gggaaattgc agagatgaaa
      721 ggggagaaaa gggatgttaa cggggtcccc ttgaagccag gggacaaggt tctatggtcc
      781 aggggggtca cctgcggcca ctgctatttc tgcaaggtga agatggaacc ttccctctgc
      841 ccccatcgct tcgtccacgg gatccacacc tcctgtgccg atccccctta cctgaccggg
      901 aattatgcgc agtacctcct tcttgatcag cgctcggatt ttttccgggt tgagaagaat
      961 ttggacctgg cgacactggt cccggcctcc tgctcaggag ccacaacggc ccatgctttc
     1021 gacctctccg gcatggagtc gggcgattcg gtcctggtcc agggggttgg cccgctgggg
     1081 atttttgcgg tcgccttcgc ccgctcctgg ggggcctccc aggttatagt catcgggagt
     1141 acggaggaaa ggttgaagat gtgccaaacc ttcggggcct cgcttctcct caaccgccac
     1201 cgcctatctc cggaagaacg gagaaaggcc attctggagg cgacaggggg aaggggagtg
     1261 gatgtggcat ttgagatggc cggagaaccc gaggccctca aagaatgcat cctttccctg
     1321 cggaacggag gagcctgcat gagcgccggc ttcggtgaac cccacggaaa gatggaagtc
     1381 gactgtttcc aggacatcgg gaggaaaaat cttcgcctcc agggaatatg ggtgagtgaa
     1441 acccgccaca cccacatggc cctacagctg gtcttgagtc ggctccgcga ttttgccggg
     1501 ctcgtcaccc accggttccc attaaacaag gccaatgaag ccctcctggc tatgaggtcc
     1561 cgggaggccg taaaggcagt ccttttaccc ttttctccat gaccaagaat aaagccaagg
     1621 aaaactcatt tttccccgat attcccttca tcgtctgccg gcgcaggaag aatcagccta
     1681 agatttctcc gctgatctgc gaaaagcgct gcccgcgcat caaaaattgc caggaatact
     1741 tcgattacgt ccaaccggcc atgtttgcgc gatatgaccg gagggaagcc agagaaaaaa
     1801 tccgagaaga ttcccttatg ataggggaag gcctacccgg ttcatccctt tctgccgttg
     1861 aggaaggttt cagaaagagg cgcccatgaa gctgtccccc gctgtgcgag agaggatcac
     1921 ccggcttctt aagggagagg ccgacacggt tcccatccta gcccagatca acgagcatgt
     1981 gttcaagctc tgcggcgggg acatgcggga agcctatacc aacgcggaga agtttgtgga
     2041 gatgaacttg gccgtcttcg agtactacca gctggatatg cctggttttt attacgacat
     2101 ttacaacatc gaagccgagg ccctgggcca gcgcctgaac tgggaagcgg accgcatgcc
     2161 ggacatcgac cgccagaacc ccctgatccg ggaacattca aacctggacc gtctgcgtcc
     2221 tccggatttt aaaaaatcgg gccgcatgcc ttacgtgctc gaggtcatgc gacgctgcta
     2281 cgatctgggg ctccccgtgc gcgttcgctt ctgctctcct ttttccctgg cagtgaacgt
     2341 tcgggggatc gagaacctgc tcctggacat cctgactacc cccctattcg cccatcggct
     2401 cctggccttc ctaaccgatg aagtcctgat tccctgggtc caggcccagc gggaggccac
     2461 cggtcagccc aaagtttccg gaaacggagc cgatgccgcg gcttctcctc ccatcgtgaa
     2521 cgtggaaatc ctggaagagt tcgtcatgcc ctatgtggcc cggatgaacc aaaggatcgg
     2581 caacgtgacc agcatgggat actgggggta cagctatctc tacaagcacc cggagaagtt
     2641 ttacaggatg ctaggcctga tggcctcggt gagcccgggg gccttgatgt gcctggaccc
     2701 ggatgtggcc aggactggac ccgagcccta cgcggagttc gcccggggga aaaagatgtc
     2761 tctcatgctg ggacttgaca cccttcttct ccaggatggg cctttgtcga agattgtgga
     2821 gcagtgccgc cggtatgtcc tggccggcgc caaagcggaa cgtttggtta tattttttaa
     2881 tgatgtgtcg attcacactc ctccgcaaaa tgtccatacc gcgatcgccg cggtccggca
     2941 ctttggaaag ctgcccatca aagaccggcc attagagtct tttcgacatt cagaggtgga
     3001 atcgttcgaa tctttcctga agaaataccg gcagcgaacg taaaggattg gaagaagggt
     3061 ttagaaaaag atggcttaga ttgtcaccgc ggtgaaagcc ggggtccaga cgtcgtcccg
     3121 acgaaagtcg ggaaccctat caaagggtgg atcccggttt tcaccgggaa tcctggattc
     3181 cggctttcgg aatgacaagt cttgcgcagt tccaactttt tacgagttca ttccgggtta
     3241 gaaagaaact tgctctgaga aaataatgaa gggggtttaa atatggcagt tcagcgagga
     3301 cgtcattttc ttcagattcc gggacctacg aatgttcccg accgagttct gagggctctt
     3361 agccagccga cgattgacca ccgaggcccg gagttttccc gcctgaccaa agaggtcctt
     3421 gaagggcttc agaaattgat caagacttcg ggaaaggtga tcgtctatcc tgcctccgga
     3481 accggctcgt gggaagcggc gctggtaaac accctttccc cgggcgatcg agttctcatg
     3541 ttcgagaccg gccaatttgc caccttatgg aagaatgtgg ccgtgaaatt ggggctccaa
     3601 gcggatttca tcccgggcga ctggcgccac ggtgcggacc cggctcagat cgaagcgaaa
     3661 ctggcggaag acccccagca ttcgatcaag gcggtcgctg tggtgcacag cgaaacctcc
     3721 acgggcgcgg ccacccggat tgccgaggtg cgaaaatcct tggatcgagc gaaacatcct
     3781 gcccttttgc tagtggatac cgtatcttct ctgggagtga tggagtaccg gcaggatgaa
     3841 tggggggtcg acgtgaccgt gagctgctcc cagaaagggc tcatgctgcc gccgggcatg
     3901 ggcatcaatg cggtcagcga gaaagcgctg gtagcctata aatcggcccg cttgcccaag
     3961 tcctactggg actggcaggc catgatcggc cccaaccaga cgggtttttt cccctacacc
     4021 ccgccgacca acctgctctt cggcctgctt gaagccctcc agatgatcca ggaagagggc
     4081 ctggagaaag ccattgcccg gcacgcccgc ctcggggaag ctacccgccg ggcggtacgg
     4141 gcctgggggt tggaaatcaa ctgcctgaac cccctggagc acactaacgg tctcaccgcc
     4201 gtgcgcgttc ccgaaggcca cgacgccgat gccctgcgca agaaaatcct ggaaaagttc
     4261 aacatgtcat tgggcaatgg tctggggaag gtgcagggca agctcttccg catcggccac
     4321 atgggggact tcaacgattt aatgcttgcc ggaaccctca gcggggtgga gatggggctg
     4381 gctctggcag gcgttccctt caagaaaggg ggagtccagg cagccataga ctatctggcc
     4441 ggaaatgagt gaacctttcg taggggcggt ccgcgaaccg ctcctgcagc cggatggtcg
     4501 atcaacagac ccgatgaaga ttctttatta cctcaccgct cacggctacg ggcatgccgt
     4561 tcgaacttcc gccatctgca atgagctttc cgggaattta cgcctgatct ttcgaaccac
     4621 catccccgag aatttttttc atgaagaagt gaggaaaccc tttgagtatc ttcccgggaa
     4681 attcgactgc ggctgtgttc agattgacag cgtcacggtc gacaaaaaag agacccttca
     4741 ggcctacatg gcgctggccg aacagaacgc cgtctgcctg gaggaggagg taaagttctg
     4801 cagggaacag aggatcgatg gaatcgtgag cgacatcact cccttggcct tcgaagtggc
     4861 ccgcaaggct ggacttcctt cggtggcagt ggccaatttt acctggtttg acatctacgg
     4921 accctacgtg cgggaatatc cgtcttttgc ccccttcctg gaaaaaatcc gccaacaata
     4981 ccagatggcc gatctgctcc tagaactaat gccctccatg gaaatgccct attttgggaa
     5041 tagagtaaga atccctccgg tagccaaaca gggacggaac aggagcggaa cgctcaagga
     5101 acacctgggt ttggagaagg acctacgcat tgctttgatc tatgtggggg aattaggaat
     5161 ggaagaaatc cgctgggggg agctaggcaa gttccgcgga tggcattttc tggggattta
     5221 ccctctcccg ggtgcatctg ccaactacca cctcatggac aaaaagacct tctcctacga
     5281 agacctggtg gcctctgccg aattgatggt taccaaaatc gggtatgggg tagtctccca
     5341 ggccctgatc aacgggactc ctctgatcta cctgcccagg gaggacttcg ccgaatttcc
     5401 cgtgctggaa aaggcaattc aggagtgggg gcatggctat cgcctttcca aagaagatta
     5461 ctatgccgtg aaatgggaaa gggtgctcga agaggtttcc agaagggaga gaccgaaacc
     5521 cgtgccttct gaagggggaa ggatatgcgc caggcaaatc gagaatttga tccgccgttc
     5581 caatccggga aaattccctg ggaaataaaa tcatgcagtt tgggaagaaa acagcggcct
     5641 ggatggtgat ttccctgttg ttgggatggt cctgggactg tgccgggatg caaccggcgg
     5701 attccatcgg ggattttttc accggggagg tgctgcggta cgaggttggc ttctggctca
     5761 tagacccggt aggcggtgga gtggcggatt tccggaacct gggtaatgga aggtacatgg
     5821 tttaccatgt gggaaaggcg gagggatttg tgggatggct gaccagctac cgccgggaaa
     5881 tctaccgttc caccatgggc acgatcaaca acggaaagag gctcatcccc ctgcgttttg
     5941 aggaatactc agtcatcggc gaatggtttc ggaagaagac gacggtttat gactatcagg
     6001 ctcacaaggt attcatggag ctggagaaag tgggggagac taagagccgg gaagagatcg
     6061 aacttccgct gggaggtctc tacgatgacc cggtgaccgc tttctataac ctccgcttcg
     6121 gagtgtacgg aaagatggaa cagggaaagg agttcctctt gaacaccctt cccagcaaac
     6181 ggccggaaat catccggatc aaggtggcct ccagagaaga gacggaaagg aggcgcgcgg
     6241 aggaaaagat aaagaccggc aaagacctcc tgattcagat tctgatcgac cgggagatgt
     6301 g
//
LOCUS       DASPRR010000529         6135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2379164, whole genome shotgun sequence.
ACCESSION   DASPRR010000529 DASPRR010000000
VERSION     DASPRR010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379164"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VEL68_19775"
     CDS             <1..805
                     /locus_tag="VEL68_19775"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXZ38275.1"
                     /translation="AAQLIEQELVDGVSLTGSVEAGSQIASLAGRHIKKVVLELGGSD
                     PFIVLEDTDIAKTARMALHARMINSGQSCIAAKRFIVMAAIARPFMEALEREVKEVKI
                     GNPMESSTTIGPLAKQDFVEVLAGQLEDAKRKGARVIYGPSPPPGEGFFFQPAIVSQV
                     EKDMRILTEEVFGPIMPVLVAQGEEDLVQMANDTPYGLGASIWTRDLDRGERIARTLE
                     AGFVAINDIVKSDPRLPFGGIKKSGIGRELSEYGLKEFVNIKSVVVKGA"
     gene            809..2443
                     /locus_tag="VEL68_19780"
     CDS             809..2443
                     /locus_tag="VEL68_19780"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HXZ38276.1"
                     /translation="MKAAELFVRQLEEEGVTCLFGLPGEENLHLLEAVRKANLKVVIT
                     RHEQAAAFMAATFGRLTGRAGVCFSTLGPGATNLVTGVVHSQLIGAPMVSISAQKPRK
                     ENWQAKFQLVDVMSLMRPITKKAFSIIDPNTIPTVLRNAFKTAEAERPGAVHIELPED
                     VAEEEASARVQERGEARRPAPDPKAIDSAVELIERAQNPLLIVSSGANRKRITPSLLS
                     FLDKTGIYVVHTQMGKGVISDECEYSLFTTGIHKRDYVNCGIDQADLIITVGYHIAEY
                     PPFLWNGKQDKTILNIDFVESELDPYFNPTVEVIGDISHSLRKIGERISGKRPFPAFA
                     KTRSLIEKKIHEYQESRYPLLPQEIVRSVREVMSPEDIVALDNGIYKLWFSRLYPTFR
                     ANTFLLDNALATMGAGLPAGIVAKMLSPQRKVLVVVGDGGFMMNSQELETAIRIQVPI
                     VILLLNDNAYGFVKWKQRNMNLENFGMDLSNPDFVKYAESFGARGIRLERGRKLTQAL
                     AQAFELNQVVLVECPIDYSVNYDVFSSELGKLVCPE"
     gene            complement(2509..3411)
                     /locus_tag="VEL68_19785"
     CDS             complement(2509..3411)
                     /locus_tag="VEL68_19785"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HXZ38277.1"
                     /translation="MHLINKLLLVALRRVPKELVRPIAMRYIAGEGLEEAVRVVRGLN
                     ARNMLATIDVLGEDVSTREEAEQAREVCARVLKTVQEEGLNSNLSIKLTQFGLKIDER
                     LCHENVKKILQVARGYGNFVRIDMEDSSITDSTLDLYERLCAEGFENVGVVIQAYLRR
                     SGEDVRKLVQSRTNTRLVKGIYVEPESIALKDREAIRGNYLKLLKMLLEARCYVAIAT
                     HDDFLVKGSFQFIQEMDLPQNAYEFQMLYGVKAKLRDQILARGHRMRIYVPFGAHWYS
                     YSMRRFQENPQMVRYILQALLVKN"
     gene            complement(3564..5435)
                     /locus_tag="VEL68_19790"
     CDS             complement(3564..5435)
                     /locus_tag="VEL68_19790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HXZ38278.1"
                     /translation="MEKKESPFFGIDPAGKSPEEIELEWFENHYHGDIPQLTVRAVLV
                     GAILGGFMSLSNLYVGLKTGWGLGVTITACILSFSIGAGLKKIGIFRTNLTLLENNCM
                     QSTASSAGYSTGSTMVGAVSALLMIQGAHLPFWTLLLWTIFLAILGVLLAIPMKRQMI
                     NREQLKFPSGIAAAQTLRALYATEREAAKQARSLGISAVLGAVMGFARANPLTWYPQV
                     LKIPAMLEIPGRITGQKLLQWTISWDMSLIMIGAGAIIGIRVAWSMLVGGLINYACLS
                     PIMYGKGVIKELGYQGIVSWSLWGGTAIMLTSGLTAFAFQWRTIGRVFSGLARIVIPR
                     ESRVRDRKLEALQNIEVPGTWVLLGMAVATVGVVSIQVISFSIHWWMGIASVVMTFFL
                     ALVACRATGETDITPIGAMGKITQLFFGVIAPARILTNLMTASVTAGAAASSADLLTD
                     LKSGYLLGANPRKQFIAQFLGIFTGAFVIVPAFYLIVPTPDVLGGDRFPAPAAQVWKA
                     VAELLAHGLSSLDITEIWALAVGGLIGILIPVLERLLPPGTRPFIPSAMGLGLAFVIP
                     FWNTLSMFTGAFIAWILTKVAKEWADRYTIPIASGMIAGESLMAVMAALLGTLGFMK"
     gene            5729..>6135
                     /locus_tag="VEL68_19795"
     CDS             5729..>6135
                     /locus_tag="VEL68_19795"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXZ38279.1"
                     /translation="MARYKWPKSKILHLKFIDEREGKTMVKIRLTALLAALSLALFVP
                     GANAAEGVAGGLGRAFTAGEIIGVHVKNPQGEMLGRITDLVMDEQGRVALVILSHGGF
                     LHINEKETAIPLNALRYDQPDNRIILDISREKLA"
BASE COUNT         1477 a         1643 c         1670 g         1345 t
ORIGIN      
        1 ggccgctcag ctcatcgaac aagaattggt ggatggggta tctcttaccg ggagtgtcga
       61 ggcaggctcc caaattgcct ctcttgccgg gcgacatatc aagaaagttg tgctggaact
      121 gggaggaagc gatcctttca tcgttctgga agatacggac atcgccaaga cggctcgcat
      181 ggctctccac gcgagaatga tcaactccgg tcaaagttgc attgccgcaa aacggttcat
      241 cgtcatggca gccatcgcga ggcctttcat ggaggccctg gaacgagagg ttaaggaggt
      301 caagatcggg aaccccatgg aatcgtcaac gacgatcggt cccctcgcca agcaggactt
      361 tgtggaggtt cttgccggtc aactggagga tgccaaacgg aagggggccc gggtcattta
      421 tggcccctct ccgcctcctg gcgaggggtt ctttttccag ccggccattg tcagccaggt
      481 ggaaaaagac atgcgaatcc taaccgaaga ggtcttcggg cccatcatgc cggtgctggt
      541 tgcccagggt gaagaagacc tagtccagat ggctaacgac actccctacg gcctcggagc
      601 ctccatctgg acccgggacc tggatagagg ggaaagaatc gccagaacgc tggaggctgg
      661 ctttgtcgcc atcaacgaca tcgttaaatc cgatccgcgg cttcctttcg ggggaatcaa
      721 aaaatcgggg atcggtcgcg agctttccga atacgggttg aaggagttcg tgaatatcaa
      781 gagcgtggtc gtcaaagggg catagttcat gaaggctgcg gagctattcg tgagacagct
      841 ggaagaagag ggcgtgacct gtctgttcgg gctcccgggg gaagaaaacc ttcaccttct
      901 ggaggctgtc aggaaggcta acctaaaggt cgtaattacc cggcacgaac aggccgcagc
      961 cttcatggcg gccacctttg gcaggctcac gggaagagcg ggggtgtgtt tttccaccct
     1021 cggtcctgga gctaccaacc tggttaccgg ggttgtgcat tcgcagctta tcggggcccc
     1081 catggtttcc atttcggccc agaagccgag aaaggagaac tggcaggcga agttccagtt
     1141 ggtggacgtg atgagcttga tgaggcccat caccaaaaag gccttctcca tcatcgaccc
     1201 gaacaccatc cccaccgtgc ttcgcaatgc cttcaagact gccgaggccg aacgacccgg
     1261 cgctgttcac atcgaattgc cggaggacgt agccgaggaa gaggcaagcg caagagtgca
     1321 ggagcggggt gaggccagaa ggccggcccc ggaccccaaa gccatcgaca gcgccgttga
     1381 gctcatcgag cgggcccaga acccgctcct catcgtttcg tcgggggcca atcgaaaacg
     1441 gatcacccca agcctgctct cttttctgga caagaccggt atctacgtgg ttcatacaca
     1501 gatgggaaag ggagtcatat ccgacgaatg tgaatacagc ctctttacca ccgggattca
     1561 caagcgtgac tatgtgaatt gtggaattga ccaggcggac ctgatcatca ccgttgggta
     1621 tcatatcgcg gagtatccgc cgttcctctg gaacggaaaa caggacaaga cgatcttgaa
     1681 catcgatttt gtggagtcag agcttgatcc gtactttaat cccaccgtgg aagtgatcgg
     1741 cgacatctcc cattccctcc gaaaaatcgg ggagagaatc tccggcaaga ggccctttcc
     1801 tgccttcgcc aaaacccgat ccttgatcga gaagaagatt cacgaatacc aggaatccag
     1861 gtaccccctc ctgccgcagg aaattgtaag gagtgtgagg gaggtcatgt ctcctgagga
     1921 catcgtggcc ctggataacg gaatctacaa gctttggttc tccaggctct accctacctt
     1981 cagggcaaac acatttctct tggacaacgc cctggccacg atgggagcag gccttcctgc
     2041 ggggatcgtg gccaagatgc tctctcctca gcgaaaggtg ctcgttgtgg tcggggatgg
     2101 cgggttcatg atgaattctc aggaactgga gacagccata cgcatccagg tgccgatcgt
     2161 cattttgctg ttgaacgaca acgcctacgg cttcgtgaaa tggaagcaaa gaaatatgaa
     2221 tttggaaaat ttcgggatgg acctttcgaa cccggatttt gtgaaatacg cggagagctt
     2281 tggcgcaagg ggaatacgct tggagcgggg acggaagctg acccaagccc ttgctcaggc
     2341 attcgaatta aatcaggttg tgcttgtcga gtgccccatt gactactcgg tcaattacga
     2401 cgttttttct tcggaactcg gaaagctcgt ctgtccggaa taaacgggga aaggctcgat
     2461 ccccggtcca ccgttgcagg aaagcctttt acatcggacg cctcccgtct aattcttaac
     2521 gagcaaagcc tgaaggatgt aacggaccat ttgcggattt tcctgaaatc gccgcatgga
     2581 ataggaatac cagtgggccc caaaggggac gtaaatgcgc atgcggtgcc ccctggccag
     2641 gatctgatcc cgaagtttcg cctttacccc atagagcatt tgaaattcat aagcgttttg
     2701 aggaaggtcc atttcctgga tgaattggaa actgcctttt acaagaaaat catcatgggt
     2761 ggcgatcgcc acgtagcatc gggcctccag cagcattttc agaagcttca aataatttcc
     2821 gcggatcgcc tcccggtcct taagggcaat ggattccggt tccacataga ttcctttgac
     2881 aagacgggta ttcgtccggg actggaccag ttttctgaca tcctctccgc ttcgccgcag
     2941 ataagcctga atgaccaccc ctacattttc aaatccctcg gcgcaaagcc gttcataaag
     3001 gtccaaagtg gagtcggtga tggaggagtc ctccatgtca atgcgtacaa agtttccata
     3061 gccgcgggct acctgcaaga ttttcttgac attttcatgg cagagccgct catcaatttt
     3121 cagaccgaac tgagttagtt tgatggagag attggagttc agcccttcct cctgaaccgt
     3181 ctttaggact ctggcacaga cttcccttgc ctgctccgct tcctcccttg tggaaacatc
     3241 ttcgcccaac acatcgatag tcgccagcat attcctggca ttgaggccgc ggacgacccg
     3301 cactgcctct tctagccctt ccccggcgat ataacgcatg gcgatgggtc tcaccagctc
     3361 tttcggcacc cgtcgcagcg cgaccagcaa gagcttgtta atcagatgca tcaagggccc
     3421 ttcacctttt gaggggttta aaaataccga aacttattat cgaatcaagc ggtggcctgc
     3481 ctgttttttc ccccgctgca tgtgctaaat gaaaagaggt tgctcttatt ttccctttag
     3541 gataggtcta aagaattttt tttctatttc ataaatccta gagttccaag aagggccgcc
     3601 attactgcca tgaggctttc ccccgcgatc atgccagaag caatcgggat ggtatatcta
     3661 tcggcccatt ccttggccac cttagtaaga atccaggcta tgaatgctcc cgtaaacata
     3721 gacaatgtgt tccagaaggg aatgacaaaa gccagcccaa ggcccatggc tgaggggatg
     3781 aatggtcggg tcccaggggg caggaggcgt tccaggacgg ggatgagaat gcctatcaac
     3841 ccaccaaccg caagtgccca gatctctgta atgtccaggc tagacagtcc atgagccaga
     3901 agctctgcca ctgccttcca tacctgcgct gccggcgcgg ggaacctgtc accccccagg
     3961 acatccggtg tgggaacgat cagatagaag gcgggaacaa tgacgaaagc accggtgaag
     4021 attcccagaa actgggcgat gaactgcttg cggggattgg ctcccagcag gtagcctgat
     4081 ttcaggtccg ttaaaaggtc cgccgaagaa gctgcggccc ccgccgttac ggaggcggtc
     4141 atcagattgg tgaggatgcg ggcaggagcg atgacaccga agaaaagctg cgtgattttg
     4201 cccatggctc caatgggggt gatatctgtt tcgccggtcg cccggcaggc tacaagggcc
     4261 aagaagaaag tcataaccac cgaggcgatc cccatccacc aatggatgga gaaactgatt
     4321 acttggatgg ataccacgcc gaccgtcgcc accgccatgc ccaagaggac ccatgttcct
     4381 gggacttcaa tattttgaag ggcctcaagt tttctatccc ggacccggct ctctcggggg
     4441 ataacaatcc tggccaggcc agaaaagacg cggccaatgg tccgccactg gaaggcaaag
     4501 gccgtcaagc cggaggtcaa catgatagcg gtgcctcccc agagactcca cgagacgatt
     4561 ccctgatatc cgagctcttt gatcacaccc ttcccgtaca ttatggggct gagacaggcg
     4621 tagttgatca atccccccac taacatgctc cacgcaacgc ggatgccgat gatggccccc
     4681 gcgccgatca taatcagact catgtcccag gagatggtcc actggagcaa tttctggccg
     4741 gtgatcctgc cgggaatctc cagcatggcc ggaatcttca ggacttgggg ataccaggtt
     4801 aagggattgg cgcgtgcaaa gcccatcacc gcccccagga cagcggagat ccccaggctc
     4861 cttgcctgct tcgccgcctc cctctctgta gcatagaggg cacgcagagt ctgcgcggct
     4921 gcgatcccgc tggggaattt gagctgttcc ctattgatca tttgccgctt catgggaatg
     4981 gccaagagga ctcccaggat ggccaggaag attgtccata ggagaagagt ccagaatggc
     5041 aaatgtgcac cctgaatcat caggagagcg gaaaccgcgc ccaccatggt actgcctgtg
     5101 gaatagcctg ctgagctggc ggtggactgc atgcaattgt tttctaagag ggtgagattc
     5161 gttcggaaga tcccgatctt cttcaatcca gcgccgatgg aaaaggacaa gatacaggcg
     5221 gtgatggtaa cacctaggcc ccatccggtt ttcaggccaa cataaaggtt ggaaaggctc
     5281 atgaatccgc ccaggatggc tccgacgagg acggccctca ccgtgagctg ggggatgtcg
     5341 ccgtgatagt ggttttcaaa ccactcaagt tcaatctctt cagggctctt cccagcagga
     5401 tcaattccga aaaacgggga ctcctttttt tccatcctct tttttctcag caaacccgcg
     5461 gcaaagtgcc ttttcaccac cttgctcgct cgtaatttcg gacggggcat tacccttgat
     5521 ctgtaggatg tttcacccca ttggaaaccc aggctctggc agcccttgcc aagctacggg
     5581 gcataaaatg tatgcatcaa aacattattt taatgcctct ttaatatact actcgaaatt
     5641 ctaccctttc ggcaatcagc ccgaaaatac gatccttcgc ggcgtaaaat gggggcttca
     5701 ccctaagcgg caccggcaag tacattggat ggcaaggtac aagtggccaa aatcaaaaat
     5761 tttgcatcta aaatttatag atgaaaggga gggaaaaaca atggtgaaaa taagattgac
     5821 agcgctgctt gcggccctca gcctggcctt atttgttcct ggagcaaatg ccgcagaagg
     5881 agtggccggc gggctgggca gggcctttac ggctggtgag attatcggag tccacgttaa
     5941 gaatcctcag ggagaaatgc tgggtaggat cactgactta gtcatggacg agcaaggaag
     6001 ggttgccttg gttatccttt cccatggggg gttcttgcat atcaatgaaa aagagaccgc
     6061 tatcccttta aatgcgctga gatacgatca gcccgataac cgtataattc tggacatcag
     6121 tagggaaaaa ctggc
//
LOCUS       DASPRR010000530         6279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1575444, whole genome shotgun sequence.
ACCESSION   DASPRR010000530 DASPRR010000000
VERSION     DASPRR010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575444"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1139
                     /locus_tag="VEL68_19800"
     CDS             120..1139
                     /locus_tag="VEL68_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HXZ38280.1"
                     /translation="MPLMTTKPMFDLAYDGGFAVGAFNVNNMEITQGIVTACAAEKAP
                     LILQISKGARKYANMRFLKHIIDAAVEENPDLPIVIHLDHGDTVDLVDACIKDGFTSV
                     MIDGSHSSFEDNVNLSAAAARAGHQKGVVVEAELGMLGGIEEHVVGLSAEEYEKNVEK
                     YLTDPKQANDFWKQTGVDSLAIAIGTSHGAYKFKHEAKLAIGRIEQIMKTCPGLPLVM
                     HGSSSVPQEYVDLVNKYGGNMPNARGVPEDQIHLAVTKYGVCKVNIDTDLRLALTAKI
                     REVFATKPSEFDPRNYLGPGRDAIVKTVKHKLQVLNCVGKAEAVIKQWKKLGEPLPAF
                     YTKSR"
     gene            1235..2248
                     /gene="fbp"
                     /locus_tag="VEL68_19805"
     CDS             1235..2248
                     /gene="fbp"
                     /locus_tag="VEL68_19805"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HXZ38281.1"
                     /translation="MQRITTVTEHLLIHQKESPMATGRFTHLLNELILAAKIISREVN
                     KAGLVDILGFTGEINVQGEEVVKLDDFANRILIHRLERAGVLCAMASEENADMIEIPA
                     AFPMGDYILIFDPLDGSSNVDSNVSIGTIFSIYRRKSPPGRTVPLSDLLQKGSEQVAA
                     GYFIYGSSTMMVYTTGEGVHGFTLDPSVGEFLLSHPDIKTPPEGKFYSVNESYWNFWD
                     EPTRKAVSYFKTPDNHRGQAYNLRYIGSLVADFHRNLLAGGIFMYPADFRDPKKPSGK
                     LRLMCEAIPLAMVVEQAGGMATDGKRRILDIDPQELHQRVPLFIGSKNDVGKVMEIFK
                     AAP"
     gene            2380..3066
                     /locus_tag="VEL68_19810"
     CDS             2380..3066
                     /locus_tag="VEL68_19810"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HXZ38282.1"
                     /translation="MSGVIVLMGGNEFRPDCEPMDRWILAGIGPKPRVVILPTAAARE
                     NPALAAENGVRYFNRLAARAEAAMIVDSATARDGKWLGLIQNADLTYLAGGDPVYLLD
                     TLRDSPAWEAILGVWMNGRTLAGSSAGAMVLGGQMWAPGQGWREGLGLLPGIAVIPHH
                     ARLAEVWKARDMLASLPEKVTLVGIEEATALLGPPWRAVGEGKVLVYEPHRVTVFTHG
                     ELINLASGFN"
     gene            3151..4422
                     /locus_tag="VEL68_19815"
     CDS             3151..4422
                     /locus_tag="VEL68_19815"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXZ38283.1"
                     /translation="MVQFNLLLSAYLAFFLLRSLTQVILNRLNVSFLRQQGGKVPDVF
                     QDVIDQEKRKKISAYTIDSANFGIFSTLASQVFFLLLLLSGFLPWLVQAIQFQGWGEI
                     VQALIFFAVLSVITNLFQIPFDLYDTFVIENRYGFNTKTLNLWVMDLLKSLALSAILG
                     GLLLWFFLSLAIHGGNLWWLWAWVLVGAFELLMIWLYPVILAPLFNKFIPVENQELTR
                     RIEALMQKAGLRSKGVFQMDASKRSRHTNAYFTGLGKSKRIVLFDTLVASHTPEEIEA
                     VLAHEIGHWKKRHVLKQLVLVETLSLVGFYLLSLLLPWPFVYQTFGLPGPVLYAGLLL
                     IGALFSPLGFFAHPLEAALSRRMERQADDYALESIPSAKPLANAFKRLAADNLSNLTP
                     HPVYAWFYYSHPPLAERILRLQNTPPKRGKL"
     gene            4891..>6279
                     /locus_tag="VEL68_19820"
     CDS             4891..>6279
                     /locus_tag="VEL68_19820"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38284.1"
                     /translation="MKSFHRTAFIGTLIFGLVFFVLPGQVLAAPQGKITVALSSDVGT
                     LDPQNHNVRISYIAGWHLYDNLVYRDQKTMKIGPHLAESWKLIDDNTWEFKLRKGIKF
                     DNGEPFNAECVKFTIERGLDPKCPQRPSVSWVKEVKVLDDYTVRIITEKPYPVALERL
                     GNYQILPAKWAKEKGADFLSTNANGTGPYRLKEWKRGVHMILEAKDDYWKGASPVKTI
                     IFRPIKEISTQISELLTGGIDIVRDVPPDQIPLIENSPMARVSKAPTLRVVYLVMDAD
                     GRAGKSQVQNLKVRQAINYAIDVDTIIKTVMTGNATRTAAVLNPQHFGYDKRLENPYP
                     YNPEKARQLLKEANFNFDQPLKLTTYTGSIQNPRALIEAVAGYLAKAGIKTNLNVYSD
                     IGMWDRLSREGKHNDMSIHSWGGGGLYDADAVYYSYFRSGEAYCFGSSPEMDKWLDEA
                     RTTMNPELRKKFY"
BASE COUNT         1378 a         1857 c         1690 g         1354 t
ORIGIN      
        1 cttcgtctct tcatccctta tctcctcaac tatgatggcc ctctcctttt ttgacttttc
       61 cccccgaccg cactatactg acttataatc attcaaatcc cacccgaagg agtccttcta
      121 tgcctctcat gaccaccaaa cccatgttcg acttggccta tgacggcgga tttgccgtcg
      181 gggccttcaa tgtgaacaac atggagatca cccaggggat cgtcaccgcc tgcgcggcgg
      241 aaaaagcccc cctgatcctg cagatttcca aaggggcccg gaaatatgcc aacatgcggt
      301 tcctgaaaca catcatcgac gcggcggtgg aagaaaaccc ggacctcccc atcgtcatcc
      361 acctggacca cggcgacacg gtggatctgg tggatgcctg catcaaggac ggcttcacca
      421 gcgtgatgat cgacgggtct cactcatcct tcgaggataa tgtgaatctg agcgccgccg
      481 ctgccagggc ggggcatcag aagggcgtgg tggtggaggc cgaactgggg atgctcggag
      541 gaatcgaaga gcacgtcgtg gggctcagcg ccgaagaata cgaaaagaac gtggagaaat
      601 acctcaccga ccccaaacag gccaacgatt tctggaagca gaccggcgta gacagcctgg
      661 ccatcgccat cgggaccagc cacggggctt acaaattcaa gcacgaagcc aagctggcca
      721 tcggccggat cgagcagatc atgaagacct gcccggggct tcccctggtc atgcacggct
      781 cctccagcgt gccccaggag tatgtggacc tggtcaacaa atacggcggg aacatgccca
      841 acgcccgggg agtgcccgag gaccagatcc acctggcggt gaccaaatac ggggtctgca
      901 aggtcaatat cgacaccgac ctgcgcctgg ccctcaccgc caagatccgt gaggtctttg
      961 ccaccaaacc ttcggaattc gaccctcgca actacctggg gcccggccgc gacgccatcg
     1021 tcaaaacggt caagcataag ctccaggtcc taaactgcgt ggggaaggcg gaagcggtca
     1081 ttaaacagtg gaaaaagctc ggagagcctc tgccggcctt ctacaccaaa tctcggtaaa
     1141 tcccggaagg ggcaggcttc aaaactgccc ctctgatcca cttcttcaag acctttttct
     1201 gatttagccc ggctttggag agaagaggtt tctcatgcag agaatcacca ccgtgacgga
     1261 acatctcctg attcaccaga aggaatcccc catggctacc gggcgattca ctcatctgct
     1321 caatgaactc atcctggcgg ccaagattat ctcccgggag gtcaataagg ccgggctggt
     1381 agacattctc ggtttcaccg gggaaatcaa cgttcagggc gaagaggtag ttaaactgga
     1441 tgatttcgcc aaccggattc tcattcaccg cttggaacga gccggagtcc tctgcgccat
     1501 ggcttctgaa gagaatgccg atatgatcga aatccctgct gcctttccca tgggagatta
     1561 tatcctgatc ttcgatcccc tcgacgggtc ctccaacgtg gacagcaatg tctccatcgg
     1621 tacgattttt tccatttacc ggcgcaaaag cccgccggga aggaccgtgc ccttgagcga
     1681 tctcctccag aaaggttcgg aacaggtggc cgccgggtat tttatctacg ggtcttctac
     1741 catgatggtg tacaccacgg gcgagggagt ccacgggttc accctggacc cgagcgtggg
     1801 ggaattcctc ctttctcacc cggacatcaa aacccctccc gaggggaaat tctattcggt
     1861 caatgaatct tattggaatt tctgggatga gcccacccgc aaggcggtct cttatttcaa
     1921 aacccccgat aatcaccgag gtcaggccta caatttacgc tacatcgggt ccttggtggc
     1981 cgatttccat cggaatctgc tggccggagg catcttcatg tatcccgctg acttccggga
     2041 ccccaagaag cccagcggga agcttcggct catgtgcgag gccattcccc ttgcgatggt
     2101 tgtggaacag gcgggtggga tggcgaccga cgggaagagg cggattctgg acatcgatcc
     2161 ccaggaactc caccagcggg ttcctctttt catcggctcg aagaatgatg tgggaaaagt
     2221 gatggagatt tttaaagcag ctccgtagtt cgtaggggcg attctcgtag agttgagtct
     2281 gcgacatgat tcgtccctgc agagttcgaa gtgaaaaacc tgtaattttt tgctcttgct
     2341 cgaaccaaat tgctccgaag gccttcagga attcgcccca tgtccggtgt cattgtcctc
     2401 atgggcggca acgagttccg ccctgattgc gagcccatgg acaggtggat cctggccggg
     2461 attggcccga agcccagagt cgtaatcctt cccacggcag ccgcccgcga gaatcccgct
     2521 ttggcggcag aaaacggagt ccgttatttc aaccgtctgg cggctagggc agaagcagcc
     2581 atgatcgtcg attccgcaac cgcccgagac gggaagtggc tcggtctgat tcaaaatgcg
     2641 gacctgactt atttggccgg cggcgatccc gtgtatctgc tggatacgct gcgtgattct
     2701 ccggcctggg aggcaatcct tggagtttgg atgaacgggc ggactctggc cggctccagc
     2761 gcgggagcca tggttttagg cgggcagatg tgggctcccg gccagggatg gagggaggga
     2821 ttgggtctcc ttcccggcat cgcggtcatc ccccaccatg cacggctggc ggaagtatgg
     2881 aaggcgaggg atatgctagc atctcttccc gagaaagtta ctctggtggg gatcgaagag
     2941 gccaccgcct tgttagggcc tccctggagg gcggtcggag agggaaaggt cctggtatat
     3001 gaaccccatc gggttaccgt tttcacccat ggggagctta taaaccttgc atcggggttc
     3061 aattaataag gagaaaaatg ttgccggttg cagggcatcc gtttttgtct ttaacccgaa
     3121 acctgaaacc cgcgaccgga aactcgcttt atggttcaat tcaacctttt actctcagct
     3181 tacctggcct ttttccttct ccgttcctta actcaggtca ttctcaaccg gctgaatgtt
     3241 tccttcctgc gacagcaggg agggaaggta ccggatgtct ttcaggacgt aatcgatcag
     3301 gaaaagcgga aaaaaatcag cgcttatacc atcgattccg ccaattttgg gatcttttct
     3361 acccttgctt cccaggtatt tttcctcctt cttctccttt cgggctttct cccctggctg
     3421 gtccaggcga tccaattcca ggggtgggga gaaatcgtcc aggccttaat tttcttcgcg
     3481 gtcctatccg ttatcactaa cctctttcag attcctttcg acctttacga tactttcgtc
     3541 attgagaacc ggtatgggtt caacaccaaa accttaaacc tctgggtgat ggacctcctg
     3601 aagagcctgg ccttgtcggc gattctggga gggttgctcc tctggttctt tctctctctg
     3661 gccatccacg gaggcaatct ttggtggctt tgggcctggg tcctggttgg agccttcgaa
     3721 cttctgatga tctggctgta cccggtgatt ctggcgcccc tcttcaataa attcatcccg
     3781 gtggagaacc aagagctaac ccggcggatc gaggccttga tgcagaaagc ggggctccga
     3841 tccaaggggg tgttccagat ggatgccagc aaacgcagcc gccacaccaa cgcctacttt
     3901 accggcctgg gaaaaagcaa acgaattgtc ctttttgata cccttgtggc atctcacacc
     3961 ccggaggaga tcgaagccgt cttggcccac gagatcggcc attggaaaaa aaggcatgtc
     4021 ctgaaacagc tggtcctggt cgaaactcta tccctggtcg gtttctatct attgtccctt
     4081 ttattgccct ggcccttcgt ctatcaaacc ttcggactcc cgggccccgt cctctatgca
     4141 ggacttctgc tgatcggggc ccttttcagc cctctgggct ttttcgccca tcccctggag
     4201 gccgccctct cccggagaat ggaacggcag gcggatgact acgcgctgga atcgatcccc
     4261 tcggcgaagc cgctggcgaa cgccttcaag agactggcgg cggacaacct ctccaatctg
     4321 acccctcacc ctgtctatgc ctggttttac tactcccacc ctcccctggc ggagcggatc
     4381 ctcagactgc aaaacacccc gccgaagaga gggaagctgt gaagaaatta ttcgaaagga
     4441 tgtgagcacg tctttcgttg cccggggaag gggaaaattc ttgccgccaa aacccggcgg
     4501 gaggcgcagg gccaagaagc acttccctgg cttccgcaga gcgacccggg ctacttgcga
     4561 aaacctttgt gaatccgaac gggtgatctt ttcggcttcc gcggattaaa taccaagcgg
     4621 tccacggaga aaccagcccc tgttttggtg gcaggcattt tctccttccc cgggcatccc
     4681 caggctacca atttcccgct gcctgaattt tttcacagct tcaggccctt cggagggatg
     4741 acctcgggac aagaaatttc ttcccctccc ccgagaatca tgttatttct ttactcgacc
     4801 ggcccccccc ggaaggcctc tactgggggt ccggaagcgc gccgctcctc gcccacgccg
     4861 aagggggaga attcctgcca ggaggtgtgt atgaaatcat tccaccgaac cgcctttatc
     4921 ggaaccctga ttttcggatt ggtcttcttc gtccttcccg gccaagtgct tgccgccccc
     4981 caggggaaga tcaccgtcgc cctgtccagc gacgtgggaa ccctggaccc ccagaaccac
     5041 aatgtccgga tcagttacat cgccggctgg catctctacg ataacctcgt ctaccgggac
     5101 cagaaaacta tgaaaatcgg cccccacctg gccgagtcct ggaagctgat tgacgacaac
     5161 acctgggaat tcaagctgcg caaggggatc aagttcgaca acggcgagcc ctttaacgcc
     5221 gagtgcgtga agttcaccat cgaacgcggt ttggacccca agtgccccca gcgaccctcg
     5281 gtctcctggg tcaaagaggt caaggtgctg gatgactaca cggtccgcat catcacggag
     5341 aaaccttatc ccgtggccct cgagcggctg ggcaattatc agatccttcc cgcaaagtgg
     5401 gccaaagaga aaggagccga tttcctgtcc accaacgcca atgggaccgg tccctaccgg
     5461 ctaaaggaat ggaagcgggg cgtccatatg atcctggaag ccaaggacga ctactggaag
     5521 ggagcctctc cggtcaagac catcatcttc cgcccgatca aggagatctc cacccagatc
     5581 tccgagcttc tcaccggggg catcgacatc gtccgggacg taccgccgga ccagatcccc
     5641 ctgattgaaa attcccccat ggccagagtc tccaaggccc ctaccctgcg ggtcgtctac
     5701 ctggtcatgg atgccgacgg acgggcggga aaatcccagg tccagaactt gaaggtccgc
     5761 caggcgatca actacgccat cgacgtggac acgatcatca aaaccgtcat gaccgggaat
     5821 gccacgcgca cggccgccgt cctgaacccc cagcatttcg gttatgacaa gcgtttggag
     5881 aatccctacc cctacaatcc ggaaaaagcc cggcagcttc tcaaggaagc caatttcaac
     5941 tttgatcagc cgctcaagtt gaccacctat acgggaagca tccaaaaccc gagggcgctg
     6001 atcgaggcgg tagccggata tctcgccaaa gcgggaatca agacgaactt gaatgtctac
     6061 tcggatatcg gaatgtggga ccgcctgagc cgggaaggaa agcataatga catgagcatt
     6121 catagctggg gcggcggcgg cctgtacgat gccgacgccg tttactacag ctatttccgc
     6181 agcggggagg cttactgctt cgggtcctcg cccgagatgg acaaatggct ggatgaagcg
     6241 cggacgacca tgaaccccga gttgcgcaaa aagttttat
//
LOCUS       DASPRR010000531         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_129844, whole genome shotgun sequence.
ACCESSION   DASPRR010000531 DASPRR010000000
VERSION     DASPRR010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129844"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VEL68_19825"
     CDS             complement(<1..1292)
                     /locus_tag="VEL68_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ38285.1"
                     /translation="MDFFQSILMGFQVALLPGNLFFCFVGVLIGTLVGVLPGLGPVAA
                     ISLLLPTTFHITPVSAVIMLAGIYYGAMYGGSTTSILVNIPGEAASVVTCLDGYQMAR
                     QGRAGPALGIAAFGSFIAGTLAVVGLMLVAPPLAEMALKFGPPEYFSLVIVGLTILTF
                     LASGPMWKALLMACFGLFLGCMGMDSIMGTTRFTFDIVELADGFGLVPVAMGLFGVSE
                     VLLNVEQTLERSIFETKIKNLLPTGKDWRDSIGAILRGSVIGFFLGILPGGGATISAF
                     TAYAVEKKVSRHSEKFGSGAIAGVAAPESANNAATGGAFIPLLTLGIPTTATMAILLG
                     ALVIYGTQPGPMLIQEHPDLFWGVVASMYVGNVMLLVLNLPLIGLWVKVLRVPYPILF
                     PLILLFCLIGAYSLNNNIAQILFMIIFGLLGYLFKKFR"
     gene            complement(1303..1767)
                     /locus_tag="VEL68_19830"
     CDS             complement(1303..1767)
                     /locus_tag="VEL68_19830"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ38286.1"
                     /translation="MPGLNNDQVSTIIWFACGGFIAWASAGYGLGTLASPGSGFITFL
                     AGLGISFFSFIGLVDATLRGRKGARWQPVMKDLDWGKAFRVLGALTAYALLLPRLGFI
                     ICTALFVGFMLRVVKPQGWPVVVAGGILAALGTYGIFELWLKAQLPRGPLGF"
     gene            complement(1778..>2280)
                     /locus_tag="VEL68_19835"
     CDS             complement(1778..>2280)
                     /locus_tag="VEL68_19835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXZ38287.1"
                     /translation="TSGVGGAQHLVMERLALKEKIKWTLIPYESGEEAVTALLGSHVE
                     ASSQTTTWKKYVDAGRFRLLVFYGEKRMTNYPSVPTLLELGYDITAEPSLMSLVGPRG
                     LSPEVVETLHQAFKKAMEDPNFIRVSNQVHQPPFYRSPQELAKHLVQIHEQLAPLIRN
                     LGLRKE"
BASE COUNT          551 a          659 c          672 g          398 t
ORIGIN      
        1 taccggaatt ttttgaacag gtatcctagc aatccaaaaa taatcatgaa gagaatctgg
       61 gcgatgttat tgttcaggct gtaggctccg atcaagcaga aaagaaggat caagggaaag
      121 aggatgggat agggcacccg gaggactttc acccagagcc cgatcagggg aaggttgaga
      181 accaacagca tcacattccc aacgtacatg ctcgccacca ctccccagaa aaggtcgggg
      241 tgttcctgga tcagcatggg accgggctgt gttccgtaga tcaccagcgc ccccaggagg
      301 atcgccatgg tggcggtggt ggggatgccg agggtaagca aaggaatgaa agcccccccg
      361 gttgcggcgt tattggcaga ctccggggcg gctacgcccg cgatggcccc gctgccgaat
      421 ttttccgaat gccgggagac cttcttttct accgcatagg cggtaaaggc cgaaatggtg
      481 gccccgccgc cgggcagaat tcccagaaaa aaaccgatga ccgaaccgcg gagaatcgcg
      541 ccgatcgagt cccgccaatc ttttcccgtg ggaagaaggt ttttgatctt cgtctcaaag
      601 atggagcgtt ccagggtttg ctccacgttc agcaagactt ccgaaacgcc gaagagcccc
      661 atggccacag ggacgagtcc aaatccgtcg gccagttcga cgatgtcaaa ggtgaagcga
      721 gtcgtcccca tgatggagtc catccccata catcccagga aaaggccgaa acaggccatg
      781 agcagggcct tccacatcgg cccgctggcc aggaaggtga ggatggttaa tcccacgatc
      841 accagcgaga aatattccgg cgggccgaac ttcagagcca tttcagccag ggggggagcc
      901 acgagcatga ggccgacgac cgcgagggtt cccgcaatga aggagccgaa ggcggcgatg
      961 cccagggccg ggccggctcg cccctggcgg gccatctggt agccgtccag gcaggtgact
     1021 acggaagccg cttcacccgg gatgtttacc aggatcgaag tggtggaacc gccgtacatg
     1081 gccccgtagt aaatcccggc cagcatgatc acggccgata cgggggtgat atgaaaagtc
     1141 gtcggtaaaa gaagggagat ggcggcaacc ggacccaggc ctgggagtac accgactaaa
     1201 gtcccgatca aaactccaac gaagcagaag aataggttgc ccggcaaaag ggcgacctgg
     1261 aagcccatca ggatgctctg gaaaaaatcc aactttcccc cgctagaagc cgagaggacc
     1321 cctcgggagt tgggccttga gccatagctc aaagatcccg taggtgccca gggcagccag
     1381 gatccctccg gcaacgacca ccggccaccc ctgcggcttt acgacccgca acatgaatcc
     1441 cacaaagaga gcggtgcaaa tgataaaccc caggcgagga agcaggaggg cataggcggt
     1501 cagggcgccc aggacccgga aagcttttcc ccaatccagg tccttcatga ccggctgcca
     1561 ccgagcccct ttccgtcccc tgagggtcgc atccaccaat ccgatgaagg aaaaaaaaga
     1621 gatgccaagg ccggccagga aggtgatgaa gcccgatccg ggagaggcga gggtccccag
     1681 accatagccc gccgaagccc aggcaatgaa ccccccgcaa gcaaaccaga tgatcgtact
     1741 tacctgatca ttattgagcc cgggcatggg tgttccatca ttcctttcgc agaccgaggt
     1801 tgcggattaa cggagccagt tgctcatgga tttgcaccag gtgcttggcg agttcctgcg
     1861 ggctccgata gaagggaggc tggtgcacct gattactcac ccggatgaaa ttgggatctt
     1921 ccatcgcttt tttgaaggcc tggtggaggg tttcaacaac ttcgggggaa agtccccggg
     1981 ggcccaccaa agacatgagg ctgggttcgg ccgtaatatc gtaccccagc tctaacaggg
     2041 ttggaacgct gggataattc gtcatccgct tctccccgta aaacaccaag agcctgaagc
     2101 gtccggcatc cacatacttt ttccaggtgg tcgtctgaga ggacgcttcc acatgactgc
     2161 ccaggagggc cgtgacggcc tcctctccgc tttcgtaagg gatgagtgtc cacttgatct
     2221 tttccttcaa ggccagccgt tccatgacca ggtgctgagc ccctcccacc ccggaggtgc
//
LOCUS       DASPRR010000532         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1419000, whole genome shotgun sequence.
ACCESSION   DASPRR010000532 DASPRR010000000
VERSION     DASPRR010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419000"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1141)
                     /locus_tag="VEL68_19840"
     CDS             complement(179..1141)
                     /locus_tag="VEL68_19840"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ38288.1"
                     /translation="MQIKKAAVLGSGVMGPGIALGFAMGGVQANLMDVDSKALERGLS
                     YVDQSLDLFIRKGMVKAADKKRIKSRISSTNNLDEGVDGCNLIIEAVTENPEVKKKAL
                     ESLDRILPPGVVIGSNTSTFPVPEMFPNIRPGDLIIIHYFNPPHIMPLVEIVRSKGTS
                     DEVIDSLRKFYRSIGKYVIVLNKFMPGFLVNRMQIGIMRELFYLYEMGIADADEIDTA
                     FKCCSALRGIVNGPFEHLDMIGLDTVKMATAFLFPGLSNMATVPKTIDKMVGEGKLGF
                     KSGEGFYKYPPHEKDAIRLRRDTALLEEAKLFHKLVEEEQIRVR"
     gene            complement(1258..2688)
                     /locus_tag="VEL68_19845"
     CDS             complement(1258..2688)
                     /locus_tag="VEL68_19845"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXZ38289.1"
                     /translation="MSQKMNIFCGNAAVEVNFPDRTRIIKAPPHQEVLAQPKEAIREA
                     LNNPIRHQPVRKLVGKGARVLIAFDDLAVPVPPMLPPDNREQVITVLLEELYAAGVQK
                     KDITLVCANGIHRMWKRTEVKTILGEAILQSFGPGQLIGHDAEDRARLSHLGFTDNGY
                     EVEVNQRVLDFDQTFYVNVNWVPFNGGWKSVMIGLGTYRSIRYIHNPEIYLQEFPASC
                     MEPDRNLLHSRIREMGTHLEKVLAQAGKKIFQIETTLNSTLPPKMSAVFCGNTPDVHE
                     KTLDHLNRNKTLAVEGQSDVLVFGLPDFMPYSMGTIINPILIARMGLGYLFSNYRNKP
                     LVKKGGMLVLSNPLIDQVDPVHHPSYKTFWEEGFARTRDAHLLYDLFADEYAHRPEFV
                     HRYRFGYGFHGAHPVQAYTTTITPKKYLGKIMAAGCKFPRVAEKLEWTPVENVEKAIE
                     VAEAEYGKDCSITYVALPPLFLPVVS"
     gene            complement(2828..>3549)
                     /locus_tag="VEL68_19850"
     CDS             complement(2828..>3549)
                     /locus_tag="VEL68_19850"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXZ38290.1"
                     /translation="ISEQDKARIEGRISFFSELSPALAGADLVIEAVPEVLELKRKVF
                     SELDLLAPPEAILATNSSTMPVSRMEDATRRPEKVLNIHFYPPIEQKNIVDLMGGSRT
                     SREVLEIAEGWIRSLHCLPLKVRKEMLGFCFNRVWHAARLEALKMWAGASVDFMDVDR
                     AWMIFTGMPLGPFAMMDGVGLDVVLQVHRFYYEEYKDPFYKPPEALEKMVERGELGMK
                     TGKGFYTYPGPAFSKADFLKP"
BASE COUNT          787 a         1014 c          884 g          864 t
ORIGIN      
        1 tttcttgtca ccaacttcgt cggccggttt tgcctgatgc gaagtggatt cagccatcca
       61 gcaatccttc tttaatcgcc taagagaagg ggcggcgtgt atccgcgcct catcgtgtcg
      121 agaaatcccc ctccccccca ttaaaatggg agggataaag gtagatgaat gcctttttct
      181 aacggacgcg gatctgttct tcctcaacca gtttatgaaa gagttttgcc tcttccagta
      241 gggccgtgtc ccgccgcaaa cggatggcgt ccttctcatg cgggggatat ttgtagaacc
      301 cctctccgct cttgaatccc agcttcccct ctcccaccat tttatcgatc gtcttcggga
      361 ccgtggccat gttggaaagt ccgggaaaca ggaaggcggt cgccatcttg accgtgtcca
      421 acccgatcat gtccagatgc tcgaagggac cgttcacgat tccgcgtaag gcggagcagc
      481 acttgaacgc cgtgtcgatc tcatccgcat cggcgattcc catctcatac aggtagaaaa
      541 gctcccgcat gatcccgatc tgcatccggt tcaccaggaa gccgggcatg aatttgttga
      601 ggacgattac atactttccg atggagcggt agaattttcg aagagaatcg atcacctcat
      661 ccgaagtccc cttgcttcgc acaatctcta ccagaggcat aatgtgaggc ggattgaaat
      721 agtgaatgat gattaagtcc cccggccgga tgttgggaaa catttcaggc accggaaaag
      781 tggatgtgtt gctgccgatg accacccccg gaggcaggat ccggtccagg gattccaggg
      841 cttttttctt gacctccgga ttctccgtga ccgcttcgat gatcaggtta catccgtcca
      901 ccccctcatc caaattgttc gtgctggaga tccggctttt gatcctcttc ttatccgccg
      961 ctttcaccat gccttttcgg atgaacaggt ccagactttg atccacatag cttaggcccc
     1021 gctccagggc tttggaatcc acatccatca ggttcgcctg cactcccccc attgcgaatc
     1081 ccaaagcaat ccccggtccc atcaccccgg aacccaacac tgccgctttt tttatctgca
     1141 tcttttcccc ctaaaatgcc agccgcaggg cctcggccca aagtgactga agctgaatag
     1201 aaaaaggggc cactctcttg tctcaacctg caatctgaca cctgcaacca aggactttta
     1261 gctgaccact ggtaagaaca ggggcggcag ggccacatag gtgatggagc aatctttccc
     1321 gtactccgcc tccgctacct cgatcgcctt ctccacattc tccaccggcg tccattcaag
     1381 cttttccgcc actctcggaa atttgcaccc tgcagccatg attttgccca ggtatttttt
     1441 gggggtgatg gtcgtggtgt aagcttgcac cgggtgggcc ccatggaacc cgtagccgaa
     1501 tctgtagcga tgaacaaact ccgggcggtg ggcatactcg tcggcaaaca gatcataaag
     1561 gagatgggcg tcccgagtcc gggcgaatcc ttcttcccag aaggtcttat aagaaggatg
     1621 gtggactggg tcgacctgat cgatcaaagg attggaaaga accagcatcc ctcctttctt
     1681 aaccaaaggc ttgtttcgat agttggagaa tagatagcct aggcccatcc gggcgattag
     1741 aatgggatta atgatggtcc ccatggaata gggcataaag tcgggaaggc caaaaaccag
     1801 gacatcgctc tgaccttcca ctgcgagcgt cttatttcgg ttcaaatggt ccagggtttt
     1861 ctcatgaaca tccggtgtat tgccgcagaa gactgcgctc atcttcgggg gcagagtgct
     1921 gtttagggtg gtctcgatct gaaagatttt ctttcctgct tgggcaagaa ccttctccag
     1981 atgggttccc atctcgcgaa tccgggagtg aagcaggttt cgatccggct ccatgcagga
     2041 tgccggaaat tcctgaagat aaatctcagg attgtgaata tagcggatgg atcggtaggt
     2101 ccccagtccg atcataaccg acttccaccc gccgttgaag ggaacccagt tgacgttcac
     2161 gtaaaaggtc tggtcgaaat ccagcacccg ttggtttact tcaacctcat acccgttatc
     2221 cgtgaatccc aggtggctca accgagcgcg gtcctcggca tcatggccga tcagctgtcc
     2281 cggcccgaag gactgcagga tcgcctcgcc caggatcgtt ttcacttcgg tccgtttcca
     2341 catccggtga atgccgttgg cgcaaaccag ggtgatatcc tttttctgta ctcctgcggc
     2401 ataaagctcc tcgagaagga cggtgataac ctgctcccga ttgtccggag gcagcatagg
     2461 agggacgggc acggcaagat catcgaaggc gatcagaacc ctggccccct tccccaccag
     2521 ttttctcacc ggctggtgac ggattggatt gttcaaagcc tcccggatcg cctctttggg
     2581 ctgggccagg acttcctgat ggggcggcgc cttgatgatt ctcgtccggt ctggaaaatt
     2641 gacctccacc gccgcattgc cgcaaaaaat attcattttt tggctcattt ttacctccca
     2701 aggcacaaac catttcaccg caaagaatac aaagtataca gagaaatgat atggaaaaag
     2761 aaacttgatg attttttaga aattatggct attccctgtg ctctcttcgc gttctgcagt
     2821 tcttccttta cggtttcaag aaatccgcct tgctgaaagc aggccctgga taagtgtaga
     2881 accctttccc ggttttcatc cccaactctc ctctttcgac catcttctcc aaagcctcgg
     2941 gtggcttgta gaaagggtcc ttgtattctt cataatagaa tctgtgcacc tgcaggacca
     3001 catcaagtcc gacgccgtcc atcatggcaa aggggcccag gggcatgccg gtgaagatca
     3061 tccaggcccg gtcgacatcc atgaagtcca cagatgcccc cgcccacatc ttgagcgctt
     3121 ccagtcgggc cgcatgccag acccggttga agcagaaccc aagcatttcc ttgcggactt
     3181 tcaagggcaa acagtgcaga ctcctgatcc agccctctgc aatctccagc acttcccggg
     3241 atgtccgact cccgcccatc aggtcgacga tgtttttctg ctcgatgggc ggataaaaat
     3301 gaatgtttaa tactttttcc gggcgtcggg tcgcgtcttc cattcgcgat accggcatgg
     3361 ttgaggaatt ggtggccagg atggcttcgg gcggggccag caggtccaac tcgctaaaga
     3421 ccttcctttt cagttccaaa acctccggga ccgcttcgat gaccagatcg gctccggcca
     3481 aagcggggga caattcggaa aagaaggaga tcctcccctc gatcctggct ttatcctgct
     3541 cggagattt
//
LOCUS       DASPRR010000533         5842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1576167, whole genome shotgun sequence.
ACCESSION   DASPRR010000533 DASPRR010000000
VERSION     DASPRR010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576167"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1458
                     /locus_tag="VEL68_19855"
     CDS             25..1458
                     /locus_tag="VEL68_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HXZ38291.1"
                     /translation="MAAQKITIEPITRIEGHGRVTIHLNEQGKVDQTFFHVDEFRGLE
                     KFSEGRPYFEMTQITQRICGICPVSHHLASAKACDGVARTEPTRPAKLLRELMHMGQM
                     VQSHGMHFFHLAAPDLVLGFDADPAERNVFGIIKADPQLALKAVNLRRYGQQIIERLG
                     GKRVHPNFAVPGGVNAPLSLKDRDAIAAELMPAVEIAKVAVGIGKGWLEANKDLAASF
                     ASFPSNYMGLVDMEGGLQLYDGEIRVKDAQGKFVAQFKPEHYLSHVAEHVEPWSFLKF
                     PYYRKLGWPKGMYRVGPLGRMNVVDKIGTPLANEEFKLFKQINGGKPVEGSLYYHYAR
                     LIELVYALERIGQLLDDSDILSSDIRAYPPSTRVPGQGVGVIEAPRGTLFHDYSTDEN
                     GQLTRVNLIVATGNNNWAMHTASGLVAKAFVDGKKLTEGVLNRVEAAIRCYDPCLSCA
                     THAIGKMPLEITLVAADGTVLDRISRS"
     gene            1541..2041
                     /locus_tag="VEL68_19860"
     CDS             1541..2041
                     /locus_tag="VEL68_19860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXZ38292.1"
                     /translation="MDLNRMLRTLVIGFGNIDRADDGVAFFVVKALRRRLGQKALNED
                     ETGLEELGAEIDSVFLSQLTPELLETLAGYQQIIFVDAHVYENVDALHCEPVSPEYTP
                     STFTHHLTPAAFLALLKALYHQEPTGHLVSLRGYDFDFHRRISAETEALVEPAVKYIL
                     QLVQDR"
     gene            2503..4809
                     /gene="hypF"
                     /locus_tag="VEL68_19865"
     CDS             2503..4809
                     /gene="hypF"
                     /locus_tag="VEL68_19865"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HXZ38293.1"
                     /translation="MENSSRENSKLRRAFELKGVVQGVGFRPFVYSLAHKYSLQGWVR
                     NSSAGVSLEVEGPPQAIEQFTEQLPLQAPPRSRIESLKFVDLPPAGLHEFEIQESQEE
                     EGQYQLISPDIATCKACQEEIRNPSDRRYRYPFTNCTNCGPRFTIIEDIPYDRPKTTM
                     ARFHMCPECRREYEDPTDRRFHAQPNACPVCGPKLQLTDPEGKPIPTADPISSAIRLL
                     KEGRILAIKGLGGFLLACDARDASAVETLRQRKHRPDKPFAIMLPDLSSIKEHCRVQP
                     EEEKLLLTPESPIVLLLWRRDSSIAQAVAPRQKYLGVMLPYTPLHHLLMKESGMALVM
                     TSGNRSEEPIAKDNEEARIRLQGIADAYLLHDRDIYVQYDDSVTAVVKGQTYVLRRAR
                     GLAPFPVRLSFSTRPILACGGELKNTFCLTRDDYAFVSQHIGDMENLETLEHFQRTVE
                     IYKKLFRIQPEVVAYDLHPEYLSTKFALAMPGKKVGVQHHFAHLAGCLAENGEEGPAI
                     GLSFDGLGYGTDGALWGGEFLVGDFRSFRRKAHFEYLPVPGGEAAIRHPWRMALSYVY
                     CLLGKERLMKVLPLFIRAAQSSRPGEEGIRIALQQIDRKMNCPLTSSCGRLFDGVSAL
                     LGLCPSISFEGQAAMELEMIADEQEKGIYEFFTEREGEKEVIHLRPLMDGVLQDLESG
                     VPPPQISGKFHNTLVKIGVTLCRKIRAEGGPEKVALSGGVFQNRLLLERMNAALESSG
                     FKVLVHRQVPCNDGGLSLGQAVIANFIN"
     gene            5011..>5842
                     /gene="hypD"
                     /locus_tag="VEL68_19870"
     CDS             5011..>5842
                     /gene="hypD"
                     /locus_tag="VEL68_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HXZ38294.1"
                     /translation="MKYIDEYRDGEKARPVIEEIHRLADQPIRLMEVCGTHTVAIARY
                     GLRNVLPPTLELISGPGCPVCVTSQGDVDVMISLSRVPEAIIATFGDMVRVPGSDSSL
                     ARERAQGRDIRVVYSPLDGMKIAIAEPQKKVIFLGVGFETTAPTIAATVLEARRQGLK
                     NFFVFSAHKTVPRALKALLELGEVKLNGFLLPGHVSAIIGSRPYEFLAQDYGLPCVIS
                     GFEPLDVLQSVLMLVRQVKNRTPRVEIQYRRGVDEGGNRKAQEMMARAFQPSDVEWRG
                     I"
BASE COUNT         1230 a         1704 c         1677 g         1231 t
ORIGIN      
        1 gtctacgatt aaaggagaaa gaccatggcg gctcaaaaga taaccatcga accgattacc
       61 cgcatcgaag gccacgggcg cgtcacgatc cacctgaacg aacaggggaa agtggatcaa
      121 accttcttcc acgtggatga attccgcggc ctggagaaat tcagcgaagg ccgtccctac
      181 ttcgagatga cccagatcac ccagcggatc tgcgggatct gtcccgtgag ccatcacctg
      241 gcctcggcca aggcctgcga cggggtcgcc cggacagagc ccacccggcc ggcgaaactg
      301 ctgagggagc tcatgcacat gggccagatg gtgcagtccc acgggatgca ctttttccat
      361 ttggccgcgc ccgacctggt gttgggtttc gatgccgacc cggcggagcg gaacgtgttt
      421 ggaatcatca aggcggaccc gcagttggct ttgaaggccg tgaaccttcg ccgctacggc
      481 cagcagatca tcgagcggct gggcggaaag cgggttcatc ccaatttcgc cgttccgggc
      541 ggagttaacg cccccctctc cctgaaggat cgcgacgcca tcgccgcaga attgatgccg
      601 gcggtcgaaa tcgccaaggt ggcggtagga atcggcaagg gctggctgga agccaacaag
      661 gacctggctg cctctttcgc ctcatttccc tcgaattata tggggctggt ggacatggaa
      721 ggcgggctgc agctctacga cggggagatc cgggtgaagg acgcccaggg caaattcgtg
      781 gcccagttca agccggagca ttatctctcc catgtggccg aacatgtgga accctggtcc
      841 ttccttaaat ttccctatta ccggaagctc ggctggccca agggaatgta tcgagttggc
      901 cccctgggac ggatgaacgt ggtggacaag atcggaacgc ccctggccaa cgaggagttc
      961 aagctcttca agcagatcaa cggcggcaag ccggtggaag ggtccctcta ttatcactac
     1021 gcgcggttga tcgaactggt ctacgccctg gaacgcatcg gccagctttt ggatgattcg
     1081 gatatcctct cctccgatat ccgcgcctat cccccctcca cccgggtccc gggccaggga
     1141 gtgggggtga tcgaggctcc tcgggggact ctctttcacg attacagcac ggatgaaaac
     1201 gggcagctca cccgggtgaa cctgatcgtg gccaccggca acaacaattg ggccatgcac
     1261 accgcttccg gattggtggc caaggccttc gtggacggga aaaagctcac cgaaggggtg
     1321 ttgaaccgcg tggaggcggc gatccgctgc tacgatccct gcctctcctg cgccacccat
     1381 gccatcggca aaatgcccct ggagatcacc ctggtggcgg cggacggcac ggtcttggac
     1441 cgcatttccc ggtcctgaac aaatttagct caatcaacgg ccggcgggag aagataagca
     1501 cttcgtccgc cggccgtttt ttcttcccgg accgctgggg gtggacctga accggatgct
     1561 gcgtaccctg gtcatcggtt tcggcaatat cgaccgcgca gacgatgggg tggccttttt
     1621 cgtggtgaag gctctgcgcc ggcgcctggg tcaaaaggct ttgaacgagg acgaaaccgg
     1681 tctggaagaa ttgggcgcgg agatcgattc cgtcttccta tcccaattga cccccgagct
     1741 gctggagact ttggccggat accagcaaat catcttcgtg gacgcccatg tttatgaaaa
     1801 cgtggatgcg ctccactgcg aacccgtatc tccggagtac accccctcta ccttcaccca
     1861 ccacctgacc ccggccgcct ttctggccct gctcaaggcc ctctaccatc aagaacctac
     1921 cgggcacctc gtgtccctcc gcggttacga cttcgacttt caccgccgca tctccgcgga
     1981 aacggaggcc ttggtggaac cggcggtaaa atatatcctc caattggtcc aagatagata
     2041 gaatcgctat acccagtccc acttttaggg gcgattcacc ttcggcccga gctcaggtcg
     2101 agggctgaat cgcccctgcg ttcttttaag accgcctttc ttgtggcccg ggggcaaggt
     2161 ctcgcctctg gcggggcagg atcggcgggc agcgaagagc ccatgaaaga tcatttcgct
     2221 tccgcagagg ggggcggtaa aagcagggcc atctgcgcac cacagggccg actccgccgg
     2281 accgatgaaa gaatgcgtgc ctcatcctgt cccgccagag acgagacctt gcccccgggc
     2341 cgggtatggt ctcctaatga tcgacattta tcagatttct ggaacgtggg ggaagggggg
     2401 acggatcatt gacggcctcg gtagtaaatg ggtatgatga tttaaacctt tgaagggaag
     2461 gtttaccttt ccccgttgga agtttagaca gcagggttga ttttggaaaa ttcttccaga
     2521 gaaaattcaa agctgcgccg ggccttcgag ttgaaggggg tcgtccaggg agtcgggttt
     2581 cgacccttcg tatatagcct tgcccataag tattccttgc agggctgggt tagaaactca
     2641 tccgcaggag tttccctcga ggtcgaaggt cctccccaag cgatcgaaca attcaccgaa
     2701 cagctccctc ttcaggctcc tcctcggtcc cgcattgaat ctctgaagtt cgtggacctt
     2761 ccccccgcag gtcttcatga gtttgaaatc caggagagcc aggaagagga gggccaatac
     2821 cagctgattt cgcccgacat tgcaacctgt aaagcctgtc aggaagagat tcgaaaccct
     2881 tccgaccgcc gctaccggta tcctttcacc aactgcacga attgcggccc gcgctttacc
     2941 atcatcgagg acattcccta cgatcggccc aagacgacca tggcccgatt ccacatgtgt
     3001 ccggagtgcc ggagggaata cgaggacccg acggaccgca ggttccatgc tcagcccaat
     3061 gcctgcccgg tgtgtggacc caaactccag cttaccgacc cagaggggaa accgattccc
     3121 accgcggacc cgatttcctc cgcgatccgt cttctgaagg aaggaaggat cctggccatt
     3181 aagggtctgg gcggatttct tctggcttgc gatgcccggg atgcatcggc cgtcgaaacc
     3241 ctccggcaga ggaagcaccg gccggacaag ccctttgcga tcatgcttcc tgacctttct
     3301 tccataaagg agcactgccg cgtccaaccg gaggaggaga aactcctcct aaccccggag
     3361 agtcccattg tccttcttct ctggaggaga gattcttcca ttgcccaagc agtcgcacca
     3421 aggcaaaaat atttgggggt tatgcttcct tataccccct tgcatcactt gctgatgaaa
     3481 gagtcgggga tggccctggt catgaccagc gggaaccgga gtgaagagcc cattgccaaa
     3541 gacaacgaag aagcccgcat tcggcttcaa ggcattgccg atgcttatct cctccacgac
     3601 cgggacatct acgtgcagta cgacgacagc gtaactgcgg tggtcaaggg ccagacctac
     3661 gtgctccgtc gcgcccgggg attggccccc ttccccgtcc gcctttcttt ctccacccgg
     3721 ccgattctgg cctgtggggg ggagttaaag aataccttct gcctgacccg ggatgattat
     3781 gccttcgtga gccagcacat cggggacatg gagaatctgg agaccctgga acattttcaa
     3841 cgtaccgtgg agatttacaa aaagctcttt cggattcagc ctgaagtcgt ggcttacgat
     3901 ctccatcccg aatatctctc caccaaattt gccctggcca tgccgggaaa gaaagttgga
     3961 gtacagcacc actttgccca tctggccggc tgcctggccg agaacggcga ggaaggcccg
     4021 gctatcggtc tctctttcga cggcctgggg tatggaaccg acggggctct ctggggaggg
     4081 gagtttctgg tgggggattt tcgttccttc cgccggaagg cccatttcga gtacttgccc
     4141 gtgcccggag gagaagccgc catccgccat ccctggagga tggccctcag ttatgtctat
     4201 tgcctattgg ggaaagagcg tctgatgaag gtccttcccc tgttcatccg ggcggcccaa
     4261 tcgagcagac ccggagagga gggcatccgg atcgctctcc aacagattga ccggaagatg
     4321 aactgccctc tcacctcctc ctgcggacgt ctcttcgatg gagtttcggc ccttttgggg
     4381 ctgtgccctt ccatctcttt tgaagggcag gcggccatgg agctggagat gatcgccgat
     4441 gaacaggaga aagggattta tgagttcttc accgagcggg aaggggagaa ggaagtgatt
     4501 cacctccgcc ccctgatgga cggggttctc caggacttgg aaagcggagt tcctcccccc
     4561 cagatttcag gaaaatttca caacaccttg gtgaaaatcg gagtcaccct gtgccggaaa
     4621 atccgcgccg agggagggcc ggaaaaagtc gccctgagcg ggggggtctt tcaaaaccgc
     4681 ctccttctgg agagaatgaa tgcggccctg gaaagctcgg gcttcaaagt tctggtccac
     4741 cgtcaggttc cctgcaacga cggcggtctc tccctcggcc aggcggttat cgccaatttc
     4801 atcaactgaa aatttcaccg cagagatcgc ggagaacgca gagaaatatc aactcaagaa
     4861 aacaaagaag gagtttttat cagaaagcca gccctgaacc gaaaatttcg ctttgttttt
     4921 ggagtttttt tcctctattc tgaaatgtct ctgcgatctc tgtgctctct gtggtgaatt
     4981 tttgcttatt tttttgcatt caagatggct atgaaataca tcgacgaata ccgggacggg
     5041 gagaaggccc ggcccgtcat cgaggaaatc caccgcttgg ccgatcaacc gatccggctg
     5101 atggaggtct gcgggactca caccgtagcc attgcccggt atggattgcg gaacgttctt
     5161 cccccgacgc tggaactcat ctccggcccc ggctgcccgg tgtgtgtgac ctcgcaaggc
     5221 gatgtggacg ttatgatctc cctttccagg gtcccggagg caattattgc tacctttggc
     5281 gatatggtta gggttcccgg gtcggattcc tccctcgccc gggaaagagc ccagggccgg
     5341 gatatccggg tggtctactc tccgctggat gggatgaaaa tagccatcgc agaaccgcaa
     5401 aagaaggtca tctttctcgg agtcgggttt gaaaccaccg ccccgacgat cgcggctacg
     5461 gtcctggaag ccaggaggca agggctcaag aatttcttcg tcttttccgc ccacaagacg
     5521 gtgccgaggg cgctcaaagc cctccttgaa ctgggagaag tcaagctgaa cggtttcctc
     5581 ctgccgggac atgtgagcgc tatcatcggc agccgcccct atgagtttct cgcgcaagat
     5641 tacggccttc cctgcgtcat ttcgggtttt gagcctctgg atgttctcca atccgttctg
     5701 atgctcgtcc gacaggtcaa gaaccgaacc ccccgggtgg agattcaata ccgccgcggg
     5761 gtggatgagg ggggaaaccg gaaagcgcaa gaaatgatgg cccgggcttt tcaaccatcg
     5821 gatgtggaat ggcgcggaat cg
//
LOCUS       DASPRR010000534         6121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_131306, whole genome shotgun sequence.
ACCESSION   DASPRR010000534 DASPRR010000000
VERSION     DASPRR010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131306"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1856
                     /locus_tag="VEL68_19875"
     CDS             198..1856
                     /locus_tag="VEL68_19875"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ38295.1"
                     /translation="MKVQKSQTCSLPQEASADMILWGGNVLTMDPQKSGARAVAIRNG
                     RFQKAGSDEEIRSLAGRNTKMIALRERTLIPGFIDSHQHLSLYGTDLLQIDCSPVRCP
                     TIGHIKKAVVREVKRRAPGEWIRGIGYDDTKTRDGKVLTRLDLDETAPDRPVFIRHVS
                     GHWAVVNSKALEIGGVREDSLDPIGGAYGRDPSGRLNGILYEQAQFAYVLEGTTGHPP
                     IIPPFPLKDRMRGLRLACDRYLASGITSVHDALVSAQTLETYQEAFKSGDCKLRVYML
                     IAIEYLPHLRALNFRTGFGNEWLRIGGIKILADGAIAGRTAYLSEPYVGSDNKGILAV
                     ESEEVLHDQIRQGHEAGFQVCVHANGDRVIEMTLSGFEKALKGLPRKNHRHRLEHCTV
                     VTPGILGRIRRLKLLVTPFGSYIHHHGEKMIPAYGAKRVEMMFAHRSFLDSGIGVSGS
                     SDHPCGPYEPLLAIQSCVTRKSSAGEILGARQRITVEEALHLYTMRSAYASFEEDLKG
                     SITPGKVADLVVLGEDPRRVDPDEIKDIPVDMTIVGGEVRYQKG"
     gene            complement(1972..2676)
                     /locus_tag="VEL68_19880"
     CDS             complement(1972..2676)
                     /locus_tag="VEL68_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38296.1"
                     /translation="MILEVDNIYTAYGLSQILFGVSLKVQDREVISLLGRNGVGKTTT
                     LRSIMGLTPPISGSIRWRGEEIVGKPTFQISRLGIGFVPEDRRIFSDLTVWENLDVAI
                     RPGGKKENAWTLERVFDLFPALRPLQKRKGGYLSGGEQQMLTIARTLMSNPDLLLLDE
                     PSEGLAPIVVHQLGEQIAKLREEGMTILLCEQNTRFSLDLSDRLYILEKGAVKYEGRV
                     QDFMKDEKIGRAYLAV"
     gene            complement(2673..3413)
                     /locus_tag="VEL68_19885"
     CDS             complement(2673..3413)
                     /locus_tag="VEL68_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38297.1"
                     /translation="MILGVEKARKSFDGFVAINGVSFSLQKGEICSIIGPNGAGKTTL
                     FNLITGHLRIDEGKLIFKDQDITNRRPYQICRLGVGRSFQRTNIFPRLTVYQNIQAAV
                     LVHRGRSFTFFQPVDSFFRKETEEILGRVGLKEYGETVSGSLSYGFQKQLELGIALAS
                     EPELLLLDEPTAGMSTQETHQTMELIGKITREKGLTLLFTEHDMEVVFSISQRIMVLH
                     QGRLIAEGTPEEVRRNPEVQKVYLGEGR"
     gene            complement(3410..4336)
                     /locus_tag="VEL68_19890"
     CDS             complement(3410..4336)
                     /locus_tag="VEL68_19890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38298.1"
                     /translation="MSKENWVPLLFIFLLLCIPLTESRFYVFLGTDILIFGLFAVSLN
                     LLLGYTGLVSFGHATYFGIGAYTCAILMKKASVPFPLAFAAAGALGGISALIIGFFCV
                     RLTKVYFAMLTLAFSQIVWAMAFKWNSLTGGDNGFVGVPFPQFLDSKVLFYYFTLGVV
                     AGSLFLLRKVVNSPFGRILTTIRENPERTEFIGIHVKLFQLVAFMISGVFAAMAGALF
                     GIFNHSVFPDILFWPASAEVLIMTLLGGIYSFFGPIVGAAVLLFVRMQVTSYTEHWPV
                     ILGFTLAFLLFFFPGGIMGFLQARIARPGGNR"
     gene            complement(4344..5231)
                     /locus_tag="VEL68_19895"
     CDS             complement(4344..5231)
                     /locus_tag="VEL68_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014514501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38299.1"
                     /translation="MPDPSFLLLQSLNGLTYAMFLFLIASGLSLIFGILGILNFAHGS
                     LYMLGAYFTQQFVSLFIDAPGQFWWAVLFASLTIAAIGAVIERFFLSHLYAREELYQL
                     LFTYALVLILADFAKIIWGTGQFSVSRPALLKGAVPILDQFFPTYNVVIIFLGPCIAF
                     TFWFTLLRTRWGRKIRAAALDREMLSSLGVHVNRLFTVVFFIGAWMAGLGGALVSPVS
                     AIVPGMDVEVIINAFIVVVIGGLGSFWGTFLGSLIVGQVYALGILVFPRLSLAFIYVL
                     MALVLIIRPWGLLGRPLQR"
     gene            complement(5308..>6121)
                     /locus_tag="VEL68_19900"
     CDS             complement(5308..>6121)
                     /locus_tag="VEL68_19900"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38300.1"
                     /translation="ISKSTAMTEPGKLHPYVFRAAANTNTEGRSAAVFMAKNPWTKIA
                     TLGPDYEFGQMVTKAFVEWMKKIKPEAQIVHQGWPKLGETDYTPFIAPLLAAKPDAAF
                     LSLWGGHFVTFAKQAKPYGFFEKVKVVAAGEGGSPETGMALKDDLPLGITTNAYDVFY
                     YPDTPEHKSYVERLKAYTKQEYAPSWAITGYIAVQFLAEAIQKAKSTDKMEVIKALEG
                     LTINTPIGKQTMRAKDHQATRGQVWGTTAKVPEYPFPILRPVEYIPADNLMD"
BASE COUNT         1462 a         1615 c         1798 g         1246 t
ORIGIN      
        1 tccactggac ccaaataccg cgttcgatac atcctttgac gaatgagatt tgaattctct
       61 ctgaatttat gtttaggagc tttgaaaatg ctcaccatcc taggggtgat taggttcacc
      121 gatggagaag ggaccaaaga acttgtattt gttttagccg aaagctgatc gctgatagct
      181 attcataaag gagagccatg aaggtacaaa agagccaaac ctgttcgtta ccgcaagagg
      241 cctcggccga tatgatcctc tggggtggga atgtcctgac tatggaccct caaaaatccg
      301 gggccagggc agtggccatc aggaacggcc ggtttcaaaa agcagggagt gatgaagaga
      361 tcaggtctct ggcgggaaga aacacgaaaa tgattgccct ccgggagagg accctgattc
      421 cgggttttat tgattctcat cagcacttgt ccctgtacgg aacggacctt ttacagatcg
      481 attgcagccc cgtccgatgc ccaacgatcg gccatattaa gaaagcggtt gtccgcgagg
      541 tcaagcgcag ggctcccggg gaatggattc ggggaattgg ttatgatgat accaagactc
      601 gggatggaaa ggtcttgacc cgcctggacc tggatgaaac ggcgccggac cgtcccgttt
      661 tcatccggca tgtgagcggt cattgggcgg ttgtcaacag caaggctctg gaaattggag
      721 gagtccggga ggattccctg gatcccattg gaggggctta cgggcgggac ccctcaggca
      781 ggttaaacgg cattctctac gaacaggcgc aatttgccta tgtcctggaa ggaaccaccg
      841 gacatccgcc tattattccc ccttttcccc tgaaagaccg gatgagggga ttacgtctgg
      901 cttgtgaccg ctatctcgcc tctggaatca ccagcgtgca tgacgctctg gtttcggccc
      961 agacgctgga aacctatcaa gaggctttca aaagcgggga ttgcaagctg cgggtgtaca
     1021 tgctgattgc catcgagtac ttgccccatc ttcgggccct gaacttccgc acgggcttcg
     1081 gaaacgaatg gctgagaatc ggcggaatca aaattctggc cgacggggcc attgctggaa
     1141 ggaccgctta cctttccgaa ccgtacgttg ggtcggataa taaaggaatt ctggccgtag
     1201 aatcggaaga ggtgctccat gaccaaatcc ggcagggcca cgaggcaggg ttccaggtgt
     1261 gcgtacacgc caatggagac cgggttatcg agatgacttt gagtggcttt gaaaaagccc
     1321 tgaagggact tccccgcaag aaccatcgcc accgcttaga gcattgcacc gtggttaccc
     1381 ccggcattct gggtcggatc cggagactca agctgctggt aacccccttc gggtcttaca
     1441 tccatcatca tggggagaag atgatccccg cttacggagc gaaacgggtc gaaatgatgt
     1501 ttgcccaccg ctcctttctc gattccggta ttggggtatc ggggtcgtca gaccatccct
     1561 gtggccctta tgagcccctc ctggcgattc aaagttgcgt cactcggaag agttcggccg
     1621 gagagatcct gggtgcgcgg caacgaatca ccgtggaaga ggctttacac ctctacacga
     1681 tgcgttctgc ttatgcgtca tttgaggagg acttgaaagg ttcgatcacc cccggcaaag
     1741 tggcagattt agtggtcttg ggcgaagatc cccggcgcgt cgacccggat gaaatcaagg
     1801 acatccccgt ggacatgacc attgtaggcg gagaggtccg gtatcaaaag gggtaggggg
     1861 tcccatggat atttcgaaaa gaccttgtga gtttttggtc gagacaaaat tcaaagatat
     1921 ctctgaggga acgatctttt tcggtcagcc ggctgaggcg ggtacggaaa atcatacggc
     1981 aaggtaggcg cggccgattt tctcatcttt catgaaatcc tgaaccctgc cttcatattt
     2041 tacagctcct ttttccagaa tatacagacg atcgctcagg tccagggaga accttgtatt
     2101 ttgctcgcag agcagaatcg tcatcccctc ttcacgcaat ttggcaatct gctcgccgag
     2161 ttgatgaacc acgatgggag ccagcccttc ggagggctcg tccagcagga ggaggtcggg
     2221 attgctcatg agagtccggg cgatagtaag catctgctgt tccccgccgc tcaaatatcc
     2281 ccccttccgc ttctgaaggg gccgcaaggc cgggaagaga tcaaaaaccc tttccagagt
     2341 ccaggcgttc tcttttttgc cgcccgggcg gatagccaca tccaggtttt cccaaacggt
     2401 caggtccgaa aagatcctcc gatcctccgg gacgaacccg attcccaacc tggaaatctg
     2461 gaaggtcggc ttcccgacaa tttcttcccc cctccatcgg atggaaccgg atatcggggg
     2521 tgtcaggccc atgatggacc tgagcgtcgt ggtcttccct actccgtttc gtcccaggag
     2581 ggaaataacc tccctatctt ggacctttag ggaaacgccg aacaggatct ggctcaagcc
     2641 gtacgccgtg tatatgttgt cgacctcaag gatcatcgcc cttcccccag gtaaactttt
     2701 tggacctcgg ggttcctgcg gacctcttcg ggagtccctt cggcgatcag ccgcccctga
     2761 tgcaggacca tgatccgctg ggagatggag aagaccactt ccatatcgtg ctccgtgaag
     2821 agaagggtaa gacctttctc ccgggtgatc ttgccgatca attccatggt ttggtgcgtc
     2881 tcctgggtgg acattcccgc cgtgggttca tccagaagga gaagctcagg ctcactcgcc
     2941 aaggcgattc ccagttctag ctgcttttga aagccataag agagtgaccc gctcaccgtc
     3001 tccccatatt ctttgaggcc gactcggccc agaatctcct cggtctcctt ccgaaagaac
     3061 gaatccacgg gctggaagaa ggtgaagctc cttccccggt ggacgagcac ggcagcctgg
     3121 atattctggt aaacggtcag ccgggggaaa atattagtac gttgaaagga gcggccgacg
     3181 cccaaacggc agatttggta gggacggcgg ttggtgatgt cctgatcttt gaagattaac
     3241 tttccttcat cgatccgcag gtggccggta atcaagttaa aaagggtagt cttacccgca
     3301 ccgttgggac caatgatgga gcagatttcc cccttctgga gggagaagct taccccgttg
     3361 atggccacaa atccatcgaa ggattttctg gctttttcca cccctagaat caccggttcc
     3421 ctcccgggcg ggcaatgcga gcctggagga accccatgat tcccccaggg aagaagaaga
     3481 ggagaaaagc cagggtgaat cccaggatca ccggccagtg ttcggtgtac gaggtaacct
     3541 gcatcctgac gaagagcagg acggccgctc ccacgatggg gccgaagaag ctgtaaatgc
     3601 cccccaggag ggtcatgatg agcacctccg cagaggctgg ccagaagaga atatccggaa
     3661 agacgctgtg gttgaaaatc ccgaatagag cccccgccat ggctgcgaag accccggaga
     3721 tcatgaaagc gaccagctgg aaaagcttta cgtggatccc gatgaattcg gtgcgctcgg
     3781 gattttcgcg gatggtggtg agaatccgcc cgaaggggga gttcaccacc ttgcggagga
     3841 gaaacaggga tccagccaca acccccaggg tgaaatagta aaataggacc ttggaatcca
     3901 ggaactgggg gaatggaact ccgacgaaac cgttgtcccc gccggtcagg gaattccact
     3961 tgaaggccat cgcccagacg atctgcgaga aggccagggt cagcatggca aagtacacct
     4021 tggtcaggcg cacacaaaag aagccgatga taagagcgga gattcctccc agggcgcctg
     4081 ccgcagcaaa ggccaacggg aaggggaccg aagccttctt catcaggata gcgcaggtat
     4141 aggcgccgat tccgaagtaa gtcgcgtggc cgaaggaaac caacccggtg taccccagca
     4201 aaaggttgag gctcaccgca aagagaccga agatgaggat gtctgtcccc aagaaaacgt
     4261 agaaccggga ttcagtaagg gggatgcaga gcaggagaaa gataaagaga agcgggaccc
     4321 agttttcttt ggacatgatt cgattaccgc tgcaaaggtc ttcctaagag accccagggc
     4381 cggatgatca ggaccagagc catcaggacg tagatgaaag ccagggagag ccggggaaaa
     4441 accagaattc ccaaggcata gacctggccc acgattaggg acccgagaaa ggtcccccaa
     4501 aagcttccca accctccgat gaccaccacg atgaaggcgt tgatgatgac ctccacatcc
     4561 atccccggga cgatggccga aaccggagaa accagggcgc cccccagccc ggccatccag
     4621 gcgccgataa agaaaacgac ggtgaacaag cggttcacgt ggacccccag ggagctcaac
     4681 atctcccggt ccagagcggc cgcccggatc ttcctccccc accgggtgcg cagcaaggta
     4741 aaccaaaagg tgaaagcgat gcagggtccc agaaagatga tgaccacgtt gtaggtggga
     4801 aaaaattggt ccagaatggg gactgccccc ttaagaaggg ccggccggga aacggaaaac
     4861 tggccggtgc cccagatgat tttggcaaag tcggccagga tcagaacgag ggcataggta
     4921 aaaagaagct ggtagagttc ttcccgggca tagaggtggc tcaggaagaa tcgttcgatg
     4981 accgcgccga tggcggcgat ggtcagagaa gcgaacagca ccgcccacca gaactgcccc
     5041 ggggcatcga tgaagaggga aacgaactgc tgggtgaaat aggcccccag catgtaaagg
     5101 gagccgtggg cgaagttcag aatccctagg atgccgaaga tcagcgagag gccggaggca
     5161 atcagaaaaa gaaacatcgc ataggtgagg ccgttcagtg actggagcag cagaaatgaa
     5221 gggtctggca tggctttatt tccccggaaa agagagaggc gcaggggcaa agcctgaagc
     5281 tctgccccat gcgtgttccg acgaaggcta atccatcagg ttatcggcgg gaatgtactc
     5341 caccgggcga aggatgggga agggatactc cgggactttg gccgtcgtgc cccagacctg
     5401 ccctcgggtg gcctggtgat ccttggcccg catggtctgt ttgccgatgg gcgtgttgat
     5461 ggtcaggcct tccaaggcct tgatgacttc catcttatcc gtggatttgg ccttctggat
     5521 cgcctcggcc aggaactgga cggcaatata accggttatc gcccagctgg gggcatattc
     5581 ctgcttggta taggctttca atcgctctac ataggattta tgctccggcg tgtcgggata
     5641 atagaacacg tcgtaggcat tggtggtaat gcccaggggg agatcgtctt tgagagccat
     5701 gccggtttcc ggagagcctc cctcgccagc ggcaacgacc ttcacctttt caaagaagcc
     5761 gtagggtttc gcttgcttag caaaagttac aaaatggccg ccccagaggg aaaggaaagc
     5821 ggcatccggt ttggcggcca gaaggggggc gatgaaaggg gtatagtccg tctctcccag
     5881 tttcggccag ccttgatgga cgatctgggc ttcgggcttg atttttttca tccactccac
     5941 gaaggccttg gtgaccattt gaccgaattc ataatccggc ccgagggtcg cgatcttggt
     6001 ccagggattt ttggccatga acaccgcggc gcttcggcct tcggtgttcg tgttggcggc
     6061 ggcccggaaa acataggggt gcagcttacc gggctcggtc atggccgtgg acttggagat
     6121 g
//
LOCUS       DASPRR010000535         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1744792, whole genome shotgun sequence.
ACCESSION   DASPRR010000535 DASPRR010000000
VERSION     DASPRR010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744792"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VEL68_19905"
     CDS             complement(<1..623)
                     /locus_tag="VEL68_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ38301.1"
                     /translation="MIKSRRPAELYQENRVLIWLCGVIFLTQLGFGSIVPVVPLYARE
                     FGVSQWAIGLTIAVYGLARFLVSVPTGQLADWVGRRWSLVLGEIVTAVGNLLCGLSTS
                     YEQFLAFRFIAGAGSAMVLTSGQISLADVATSANRGRVMGIYQGVFLFAVGFGPLPGG
                     VLAERFGLNAPFFVFAVLGALAGVVAFTQVPETRGFREKQMGDAAGPA"
     gene            921..2648
                     /locus_tag="VEL68_19910"
     CDS             921..2648
                     /locus_tag="VEL68_19910"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HXZ38302.1"
                     /translation="MGMLSKDQESLLKEERGLMNDLRTALVKFEAEPEDEGILLESIR
                     QLDELFLLVVVGEFNSGKTAVINALLGQPLLEEGVTPTTTRIYLIRHGEQGRNVLSED
                     QILVTFPVEFLSDMTIVDTPGTNAVIRRHEAITSHFVPRSDLVLFVTSADRPFTESER
                     GFLERIRDWGKKVVFVINKIDILEKEEEITKVKDFVLENARLLLGVVPEAFALSARGA
                     LRAKQGEARLWEKSRFAPFEEYLHNTLDESSRLQLKFLNPLGVGARLTEKYLGKVDSR
                     TASLRTDLDLLAAVESQLKFYQEDMQRNFGFRMADIDNILLGMEKRGQVFFDETFRLG
                     RVFDLLSKPRIQQEFERQVVADLPRQVEDKVTQLIDWLVESDLRQWQAIHQHLSGRRR
                     EQSERIIGDMGPGKFSYDRDRLVEAVAREARRVVDGYDRDREAKTVADHARTAVAASA
                     ALEAGALGLGALVTTLATTAAADVTGVLLAGLVAAVGLIIIPARRRQGKAKLNEKIAA
                     LRAQLSGSLRGQFAKEVQHSLERIQEAIAPYTRFVRSERERMEEMGKKLKDLKTGIER
                     LSKKIESLS"
     gene            complement(2667..>2865)
                     /locus_tag="VEL68_19915"
     CDS             complement(2667..>2865)
                     /locus_tag="VEL68_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38303.1"
                     /translation="KGAGLHESILGSGVVLGPLLGGILAHSTGLRAPYILCLAVLAIV
                     VIIEVSLIKKNPQISQIAQIR"
BASE COUNT          665 a          842 c          818 g          540 t
ORIGIN      
        1 gcaggcccgg ctgcgtcccc catctgcttc tccctgaagc cccgggtttc cggcacctgg
       61 gtgaaagcca cgaccccggc aagtgccccc agaacggcga agacgaagaa gggggcgttg
      121 agcccaaaac gctcggccag caccccgccg ggcaaagggc cgaaccccac cgcgaaaaga
      181 aacacccctt ggtagatgcc catgacccgt ccccggttgg ccgacgtggc cacatctgcc
      241 agggaaatct gccccgacgt gagcaccata gcagagcccg ctccggcgat gaagcgaaac
      301 gccaggaatt gttcgtagct cgtggaaaga ccgcagagga gattccctac cgcggtgacg
      361 atctccccca acaccagcga ccagcgccgt cccacccagt cggccagctg gcctgtgggc
      421 acactcacca ggaaacgggc caggccatag accgcgatag tcaacccgat ggcccactgg
      481 gaaacgccga actcccgggc gtacaatgga accacgggca cgatgctccc gaagcccagc
      541 tgggtcagga agatcacccc gcacaaccag atcaggaccc ggttttcttg gtataactcg
      601 gctggcctcc tgctctttat caacggattt tccgtcccct ctcacctctc aggtttggga
      661 ccctgcaaga tgtcccttgc atggatgcgc atacttgagt aaagatagca tgaaacttca
      721 ttccgcaaga atggaaatac cggggagaag cgatgaaaaa tctgaactgg caaaatatta
      781 tcgtagtttc cttggtcgtc ctttcattat cttctatgtc ctttctgcat aatctgcgta
      841 atctgcggat aatctgcgga taaacgctgg gaaaaccccc tctccctgta tataatggaa
      901 ccggaaagag aggccattcc atgggtatgt tgagcaaaga tcaggagagt ctcttaaagg
      961 aagagcgggg gttgatgaac gacctgcgaa ccgccctggt caagtttgaa gccgaaccgg
     1021 aagatgaggg aattctgctc gaatcgattc gccagctcga cgaactcttt ctcctggtag
     1081 tggtcggcga attcaattcg ggaaaaaccg cggtcatcaa cgccctcctg ggacagccgc
     1141 tgctggaaga aggcgtgacc ccgaccacaa cccgaattta cctgatccgc cacggcgagc
     1201 aggggaggaa cgttctcagc gaagatcaaa ttctggtcac ctttcccgtg gaattccttt
     1261 ccgacatgac cattgtggac actccaggca ccaacgccgt catccgccgg cacgaggcga
     1321 tcaccagcca tttcgtccct cgatcggacc tggtactctt cgtcacctcc gccgaccgtc
     1381 ctttcactga gagcgagaga ggcttcctgg agcgcattcg ggattggggg aaaaaggtgg
     1441 tcttcgtcat caacaagatt gatatcctcg aaaaagaaga agaaataaca aaagtcaagg
     1501 atttcgtcct ggaaaacgcc cgcctcctcc tgggagtggt ccccgaggcc tttgccctca
     1561 gcgcccgtgg ggctctgcgg gccaagcagg gggaggcgcg gctttgggaa aagagccgct
     1621 tcgccccttt cgaggaatat ctccacaata ccctggatga aagcagccgc ctgcaactga
     1681 aatttctcaa ccccctgggg gtgggggccc gcctaactga aaaatatctg ggcaaggtcg
     1741 attcccggac ggcttccctc agaaccgacc ttgatcttct ggccgccgtg gaatcccagc
     1801 taaaattcta ccaggaggat atgcagcgca acttcgggtt tcgcatggcg gacattgaca
     1861 atatcctcct gggaatggaa aagcgagggc aggtgttttt cgatgagact ttccggctgg
     1921 ggcgggtttt cgacctcctc agcaagcccc gcatccagca ggagttcgaa cgccaggtcg
     1981 tggcggattt gccgcggcag gtcgaagata aggtgaccca attgatcgac tggttggtgg
     2041 aatcggattt acgccagtgg caggccatac atcagcacct ttccggcaga cgccgagagc
     2101 aaagcgaaag aatcatcggg gacatgggcc cgggaaagtt ctcttacgac cgcgaccggt
     2161 tggtggaggc agtggcccgg gaagcccgcc gggtggtaga cgggtacgac cgggaccggg
     2221 aggcaaaaac cgttgcggat catgcccgga ccgccgtcgc ggcatcggcg gcgttggaag
     2281 cgggcgccct cggactgggg gcgctggtca cgacgctagc cacgacagcg gcagcggacg
     2341 tgaccggtgt tctcctggcc ggcctggtgg ccgccgttgg cctcatcatc attcccgccc
     2401 gccgccggca ggggaaggcg aagttgaacg aaaagatcgc cgcccttcga gctcagctca
     2461 gtggctcctt gcggggccag ttcgcaaagg aggtccaaca cagcctggag cgcatccagg
     2521 aagctatcgc cccctatacc cgtttcgtgc gctcggaacg ggaaaggatg gaagagatgg
     2581 ggaaaaagct caaagatttg aaaaccggaa tcgaaaggtt gagcaaaaag atcgaatcgc
     2641 tatcctgacc tttctgtttt gcgcctttac ctaatctgtg caatctgcga aatctgagga
     2701 ttctttttaa tgagacttac ctcaatgatt accacaatgg ccaggaccgc tagacagagt
     2761 atgtagggag ccctcagccc ggtggaatgg gccaggatcc ccccgagaag gggccctaat
     2821 actactccgc tccccaggat ggattcgtgc aggccagctc ccttc
//
LOCUS       DASPRR010000536         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1422049, whole genome shotgun sequence.
ACCESSION   DASPRR010000536 DASPRR010000000
VERSION     DASPRR010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422049"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VEL68_19920"
     CDS             <1..1059
                     /locus_tag="VEL68_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38304.1"
                     /translation="GGLLGRQVELIIVDDEMKAEKGAAAVEKLVTVDKVDILVGGMAS
                     GVHLGQIPIMKKYEKITVWIGAASSRCEQAMGPVDWYFHLHPWDYQQGASYQEGWSEM
                     AAKRPEVKIVKWFNANEEGAFGTASFKAGQAAFKTWKTPKGEQVTLEGEPFKSAALGG
                     GDYRTVLRHAKEAKPDIFLWTGYDADALPIMEQAKEVGFAPPIFIGSPPGWPIGFGKS
                     PLAEGVTLYGMWAPSIKEVSPVSKHFWEAYNKEYNQEPATYFAPLAYTNAYFVAEGIK
                     KAKTLEKAALIKALQETKYVSPVGETLAIKPSNVIKNQGFTKQKILQWQKGEQQVIWP
                     FEFSTAKLAYPFPAWDKR"
     gene            1331..>2048
                     /locus_tag="VEL68_19925"
     CDS             1331..>2048
                     /locus_tag="VEL68_19925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38305.1"
                     /translation="MDFLSKLKQSNYQIVVITAVLVVVLAFWKPHVLVEGLQRGCLYA
                     VIALPMALILGIVGIINLAHGEYMMLGAYFAYWLNVYTGVDPLVAILPALAAFFLIGI
                     ASYRAIIKYVLGTPELNQLLLTFGLAMILEQLANLLWTSRPIKLPLPYVSSSATIGSF
                     SFGTYEFIYVGAAILVLAGLLLFLKRTPTGQAALAVGQNPRGAKLVGIHVDRTYLLIF
                     SLSIALIGALGAIFMTRHSIF"
BASE COUNT          435 a          605 c          595 g          413 t
ORIGIN      
        1 ggcgggctgc tgggccgcca ggtggagttg atcatcgtcg atgacgagat gaaggcggaa
       61 aagggcgcgg ccgccgtgga gaagctggtc accgtggaca aggtggatat cctggtgggc
      121 ggcatggcca gcggcgttca cctgggtcag atccccatca tgaagaaata cgagaagatc
      181 acggtatgga tcggggccgc ctcttcccgc tgcgaacagg ccatggggcc ggtggactgg
      241 tacttccacc tccacccctg ggattaccag cagggggcca gctaccagga ggggtggtcg
      301 gaaatggcgg ccaagcggcc ggaagtgaaa atcgtaaaat ggttcaacgc caatgaggaa
      361 ggagccttcg gcactgcatc cttcaaagcg ggccaggcgg catttaagac ctggaaaacc
      421 ccgaagggag agcaggtgac gctggaagga gaacccttca agagcgcggc cctgggcgga
      481 ggcgactacc gcactgtgct ccggcacgcg aaagaggcca aaccggacat cttcctctgg
      541 accggttacg atgccgatgc cttgccgatc atggagcagg ccaaggaagt cggtttcgca
      601 ccccccatct tcatcgggtc ccctcccggc tggcccatcg gcttcggaaa gtctcccctg
      661 gcggaaggtg tgaccctcta tgggatgtgg gccccctcca tcaaggaagt cagcccggtg
      721 agcaagcatt tctgggaggc ctacaataag gagtacaatc aggaacccgc cacctatttc
      781 gcccccctcg cctacaccaa cgcttatttt gtcgccgagg gcatcaaaaa agccaagacc
      841 ctggagaagg ctgccttgat caaggcgctc caggaaacga agtatgtctc cccggtggga
      901 gagacgctgg ccatcaagcc gagcaacgtg attaagaacc agggattcac caagcagaag
      961 atcctccaat ggcagaaagg ggagcagcag gtcatctggc cctttgaatt ttccacggcc
     1021 aagctcgctt atcccttccc agcgtgggat aaaagataag aaacgcatag ggcatagcgc
     1081 agagagcata gcgaacatcc agacaatatt ggaatccccc tcaccctatc ctctcccgcg
     1141 agagactgtg tcgcaattgg gggaaagagt cgtcattccg gcgaaagccg gaatccagtg
     1201 ttttcaaagc ctaacgaact aactggaccc cggttttcac cggggtgacg aataaaattc
     1261 gattctttca cgccttcagg gagggaaggg cgggggatgt gagggcttag gcgcattttc
     1321 ggagtcggca atggatttcc tcagcaagct gaagcaaagc aattatcaga ttgtagtcat
     1381 tacagccgtc ctggttgttg tccttgcctt ctggaaaccc catgtgctgg tcgagggtct
     1441 gcagagaggc tgtctttacg cggtaatcgc tctgcccatg gccctcatcc tggggattgt
     1501 ggggatcatc aatctggccc atggggaata catgatgctg ggggcttact tcgcctactg
     1561 gctgaacgtt tacaccgggg tggatcccct cgtggccatc cttcccgccc tggccgcttt
     1621 cttcctgatc ggtatcgcca gctaccgggc catcatcaag tatgtcctgg gcaccccgga
     1681 gcttaatcag ctcctgctga ccttcggcct ggccatgatc ctggagcagc tggccaatct
     1741 cctgtggacc tcccgtccca tcaagctgcc cctgccctat gtctcttctt cggccacgat
     1801 cggttctttc agcttcggga catacgaatt catttatgtg ggcgctgcca tcctcgtcct
     1861 ggccggactc cttctcttcc tgaagcgcac ccccacaggc caggccgccc tggccgtggg
     1921 acagaacccc cggggggcca agctggtggg gatccacgtg gaccggacgt acctcctgat
     1981 cttcagcctt tccatcgccc tgatcggggc cctgggtgcg atcttcatga cccgccattc
     2041 catcttcc
//
LOCUS       DASPRR010000537         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_134049, whole genome shotgun sequence.
ACCESSION   DASPRR010000537 DASPRR010000000
VERSION     DASPRR010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134049"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1153)
                     /locus_tag="VEL68_19930"
     CDS             complement(245..1153)
                     /locus_tag="VEL68_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXZ38306.1"
                     /translation="MLEGKVAVVTGAGRGIGREIATLLAKEGAKVVVNDFGGREDGTG
                     GESKVADDVVKDIKAAGGAAVGNYDAVGSMGAGKRIIQTALDSFGKLDILVNNAGILR
                     DRMIFNMTEEEWDGVIQVHLKGTFACTRAAVPLMREQKSGRIINFTSTSGLVGNVGQA
                     NYAAAKLGIVGFTKVVALDMAKYNVTANCISPFAWTRLIGTIPTETEVQKKRVEKLKK
                     MSPADIAPLVAYLASDEAQYVSGQVFGVRGKEIFIFSQPRPVRSIHHSEGWTPDRLSE
                     MFKGSLGMHLVPLETSGQVFSYDPLV"
     gene            1388..>2406
                     /locus_tag="VEL68_19935"
     CDS             1388..>2406
                     /locus_tag="VEL68_19935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HXZ38307.1"
                     /translation="MRDDRPFLVLRKHRGNRFTLPVLLGCAEEKGLSKDFRVLLANSK
                     DEIAQKIMGAAALIGYSFMTPNLEEVREEVKWLRGNLPGAPIFMAGGSHATGDPEGTL
                     ALGFDFVFAGEAEETFPGFLHRFLGGENPSGKILEADHRKDLDLVHPPHSLGHRFFAP
                     IEITRGCPYGCTYCQTPRIFGHRLRHRKPENVQFYLRQAIPHGYLQTKFISPNAFSYG
                     SKTPGEPDLEKLGNLLALCQGTGLEGIHFGCFPSEVRPDWVTPGVLELVKKYCRNRTI
                     VLGAQSGSDLLLEKLKRGHTADQALNAARWIRQAGFRPHVDFVFGFPGETMEDRKLTL
                     QLIDK"
BASE COUNT          540 a          679 c          633 g          554 t
ORIGIN      
        1 ttaaaaattc ggcatctttg ggggaaaggt tgaaacgcac gcaggcctgt tccaccaaag
       61 tcggaagagg ggtctttcct tcctctctct ccttggatat ccattgaacg gctttccgca
      121 tgcttgtccc ttcaggcaaa atggatgaca tccgatacct cctcaagaaa agttctgcaa
      181 ggggcaaaga aagatcgtct cgtaaaaagt ctgaaccccc ttttttcggt tatggactca
      241 caactcaaac cagaggatcg tagctaaaga cctgtccgga cgtttccaga ggaaccagat
      301 gcatgcccag ggatcctttg aacatctcgg aaagacgatc cggggtccac ccttcggagt
      361 gatggatgga gcggaccggc ctgggctggg agaagatgaa aatctctttg ccgcgcacac
      421 cgaagacctg accggacacg tactgagcct cgtcgctggc caggtatgcc accaagggtg
      481 cgatatccgc ggggctcatc ttcttcaatt tttccacccg tttcttttgg acttccgttt
      541 ccgtggggat cgttccaatg agccgagtcc aggcaaaggg ggagatgcag ttagcagtca
      601 cgttgtattt ggccatgtct agggctacaa ccttggtgaa gcccacgatt cccagcttgg
      661 ccgcggcgta gttggcctga cccacattgc caaccagacc cgaggtggag gtaaaattga
      721 tgatccgccc gctcttctgt tcccgcatca ggggcacggc cgcccgggtg caggcaaaag
      781 tccccttaag atgaacctgg atcaccccat cccattcctc ctcagtcatg ttaaagatca
      841 tccggtcgcg gaggatcccg gcattgttga ccaggatgtc cagtttaccg aaactgtcca
      901 gggccgtttg gatgatcctt ttgccagccc ccatggagcc cacggcatca tagttcccca
      961 cggccgcgcc cccggcggct ttaatatcct ttaccacatc atcggctacc ttggactccc
     1021 cgccggttcc gtcttcccgt ccgccgaaat catttaccac cactttcgcc ccctctttag
     1081 cgagaagggt tgcaatttcc cggccgattc cccttccggc cccggtcacg actgccacct
     1141 tgccttcgag catcattgat aggtcctccc tgtggtttgg aataaaatga taaagacccc
     1201 cgggtatctg actttccggg acgaggttta tatagcagaa aatggggagg gatacaagac
     1261 tgctaaaatg cggattaccc gttcctggtt ctccgttgtc cggtcaaaaa gaagaaccgg
     1321 cagctgggaa caggccactc agattttttc ttttgacagg gcgaattcaa cggggctata
     1381 aaaaaatatg cgtgatgatc ggcccttttt ggttcttcga aaacaccggg ggaatcggtt
     1441 cacccttccc gttcttctgg ggtgcgcgga agaaaaaggt ctttcgaaag atttccgggt
     1501 gctcctggcg aactcaaagg acgaaatagc ccagaagatc atgggggcag cggccctaat
     1561 cgggtattcc ttcatgactc cgaatttgga agaagtccgc gaagaggtga aatggctgag
     1621 aggaaacctc cccggagcgc ctatttttat ggctgggggg tcccacgcca ccggtgaccc
     1681 cgaaggaacg ctagccctgg gatttgattt cgtttttgca ggggaagcgg aggagacctt
     1741 tcccggtttt cttcatcgct tcctgggagg agaaaatcct tccgggaaaa tcctggaagc
     1801 ggaccatcgg aaagaccttg atctggttca tcctccccat tcgctgggac accgcttctt
     1861 cgcccccatt gaaatcaccc ggggctgccc atacggatgc acgtattgtc aaaccccccg
     1921 aatttttggc caccggctcc gtcatcggaa accggaaaac gtgcaattct atctaaggca
     1981 ggccattccc cacgggtacc tccagaccaa gttcatctct cccaacgcct tttcctatgg
     2041 ctcgaagact cccggcgaac ccgaccttga aaaattggga aacctgttgg ccctctgtca
     2101 gggaacgggc ctggaaggga ttcatttcgg ttgtttcccc tccgaagtcc ggcccgattg
     2161 ggtcactccg ggggtcctgg aattggtgaa gaaatattgc cggaacagaa ccatcgtcct
     2221 gggcgctcaa tcgggcagtg atctcctgct ggaaaagctg aaacggggtc atacggcaga
     2281 tcaggccctg aatgccgccc gctggattag gcaggccggc ttcaggcccc acgtggactt
     2341 cgtatttggg tttcccgggg agacgatgga agatagaaag ctcacgcttc aattgatcga
     2401 caaaat
//
LOCUS       DASPRR010000538         7142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1422295, whole genome shotgun sequence.
ACCESSION   DASPRR010000538 DASPRR010000000
VERSION     DASPRR010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422295"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VEL68_19940"
     CDS             <1..822
                     /locus_tag="VEL68_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXZ38308.1"
                     /translation="LPETLVESILFGHEKGAYTGADRPRDGLILQADGGTLFLDEVGE
                     LPLSVQRSFLRVLQEKKFRPVGGKNEIQSDFRLIAASNRDLDQMVGQNRFREDLLFRL
                     RAFMIEIPPLRERKEDIRDLLSHYTVELCDRYRIEGKDFSPEFMDLLLGYPWPGNVRE
                     LIHALERTLISARNERVLFPKHLPTHIRVQVARSSVGEKEPESSSLAPSPPAFPTIQE
                     MRDRVVDEAERKYLHDLLSFTRGNIQEACRISGLSRSRFYLLLKKYKISKSFSPA"
     gene            1334..1558
                     /locus_tag="VEL68_19945"
     CDS             1334..1558
                     /locus_tag="VEL68_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38309.1"
                     /translation="MKYQRDGRVFEIAKITRDFLVLQALDDSTQVLTGKASLAFLFAE
                     LPQSGVPDVPMLPGRIGPLLAPMNRLEAPR"
     gene            1720..2142
                     /locus_tag="VEL68_19950"
     CDS             1720..2142
                     /locus_tag="VEL68_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38310.1"
                     /translation="MKVLIHTMEAAESLVKMKKAIETALPNIKGETFSTLESLAQRLK
                     EPYDGEEKVALLAATDQKNLEDFLSLQRLFLNVPLILLVPNRSTETMALAHRLRPRFL
                     NDVQGDFTAVIEVLRKMVAVQGERGAADSPPKSRLGLL"
     gene            2186..2650
                     /locus_tag="VEL68_19955"
     CDS             2186..2650
                     /locus_tag="VEL68_19955"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXZ38311.1"
                     /translation="MYNKILVPLDGSALAECIFPHLETLAGGCQVKEVVFIRVVEPFR
                     QPTPSEYIIKPEEIIRINQEAKAEAEKYISQASAKIKRDGFRVKTEVVVGSPAELVAD
                     YATKNGVDLIVIATHGRSGVSRWVWGSVADRILRSACVPVLMVRAPGCVPGI"
     gene            3066..3254
                     /locus_tag="VEL68_19960"
     CDS             3066..3254
                     /locus_tag="VEL68_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HXZ38312.1"
                     /translation="MGVERISPEEARQGLESGRLLLVCAYEDENRFRQMQLEGAISFR
                     EFQTKLPTWGKGQEIVFY"
     gene            complement(3676..4659)
                     /locus_tag="VEL68_19965"
     CDS             complement(3676..4659)
                     /locus_tag="VEL68_19965"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXZ38313.1"
                     /translation="MNSFEHIETQDLASALTILQEPRTAALAGGTDLLGELKRGIQKP
                     DRLIDLKRLSSLKGVSMRPDLRLGALTPLSMVEEHPAVISQWPLLHQALKSTATLQLR
                     NMGTVGGNLCQRPRCWYYRNHLSLCWLKGGEFCWAARGENHYHTILGRAICHASHPSD
                     LAPALMALQSRIKIASPTGMREIALEELYQEPRPERRQMTILEPGELITEVAVPLPGR
                     GSRGIYLKAMERKAWSFALVSVAAILTFDGDTITEGRLVLGGMATIPWRAKEAESILG
                     GKKFSAGLCREVAESALQEARPLRDNHYKVPLAKALIRRALTQLADQHGTL"
     gene            complement(4652..6985)
                     /locus_tag="VEL68_19970"
     CDS             complement(4652..6985)
                     /locus_tag="VEL68_19970"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXZ38314.1"
                     /translation="MMSQVIKVKSEFEGHLYEEYIVVEGEGLSAWEAGRSLKFVGQGK
                     TRIDGRERVTGQARFTSDIQLPGMLYGKVLRSPLPHGRIKAIETQKAEGVPGVRAVLT
                     YQNIPPLPFFGGQTYILDPTVRYAGDEVACVIAEEEEICADALERIAAEYDPLPFVLD
                     AEEALRPDAPRLHPGGNLWKGAPDLYERGNLETGFREAEVIVEEVFKTQTALHNCLET
                     HGSVALWEGDRLTLWDSTQHIFGVRSQAAALLNLPLDKVRIIKKYMGGGFGSKNFMGK
                     YSILAAIAARRTGRAVKILLDRHEENLAAGNRPASTQYLKAGAKKDGTLTAFHLRAYT
                     AAGAYALGAPAVGGPVRQLYACPHVKTEQYLVFTNTGPLSAFRGPGYVEGTFTLESIM
                     DELAEKLGMDPLSLRLKNYTDLNQTTAKPYSTKGLREAYTTGAQKIHWEDRPPEGQGN
                     KRRGRGMASQVWGGSGGPPAYALVKINPDGTVTVISGTQDLGTGTKTVLAQIAAEELG
                     LEMEKVSVELGDTQLAPYAPISAGSMTLPSVGPAVRVAAHDAKRQLLDVAAQVLEIPR
                     EPLNIQEGFLRSPALQKPAAIRDVLSNVRNFMIIGQGARQPNPEKANVNTFGAQFVEL
                     EVDTDTGEVKIEKVVAVHDSGRVINPLTLSSQITGGVLQGLGFALTERRFIDRRAGIV
                     VNGNLEDYKIPTCLDVPPLEQDMVDRPDLQANNLGSKGVGEPPIIPTAAAVANALADA
                     LKVRIKELPITREKVLEALGQGKDSGVRQGQKETAHE"
BASE COUNT         1496 a         2042 c         2068 g         1536 t
ORIGIN      
        1 ctgccagaga ccctggtcga atccatcctt ttcggccacg aaaagggagc ttataccgga
       61 gccgaccggc cccgggacgg cttgatcctc caggccgatg gcggaactct gttcctggat
      121 gaagtggggg aactcccgct ctccgtccag agaagctttc tccgcgtact gcaggaaaag
      181 aaattccgcc cggtgggcgg gaaaaacgaa atccagagcg atttccggct catcgccgct
      241 tccaaccgcg acctcgatca aatggttggg cagaaccgct tccgggaaga cctccttttc
      301 cgccttcggg ccttcatgat tgagattcct cctctacggg aacgaaagga ggatatccgg
      361 gatttgctat cccattacac ggtcgaatta tgcgaccggt acagaattga ggggaaggat
      421 ttctctcccg aattcatgga cctcctctta ggctatccct ggccgggtaa cgtccgggaa
      481 ttaattcatg ccctggaacg gacgctcatt tccgcaagga acgagcgagt cctttttccc
      541 aagcatctcc ccacccacat tcgcgtgcag gtggcccggt cgtcggtggg tgaaaaagag
      601 ccggaaagct catccctcgc tccctctccc ccggcctttc ccacgattca agaaatgcgg
      661 gatagagtgg tcgatgaggc agagaggaaa tacctgcacg atttgctctc ctttacccgg
      721 ggcaacatcc aggaagcctg ccggatttcc ggcctctccc gatcccgttt ctatctgctc
      781 ctgaaaaaat acaaaatctc caaatccttt tctcccgcct gatccgcctt ttgtcctctg
      841 gagttcgaag gattcctctc ctggaagact ttttcctctt tttcaacacc gatttacccc
      901 ccccttaagt cagcagacca agagattcct tttaaaatcg agggattttg ctcttcggtt
      961 ctttggcatg ctttttggaa tgtgaagaca aggagggaaa tccccgtcgc ttatctaaat
     1021 gcggtccaag ccaagagagg ggaaacaaag ccggtcgcat cctttgcttt catcggattc
     1081 aaagagaccg ttccaggagg taatcatgaa gtgtcaacgc tgccaggggt ggatggttga
     1141 ggaaagatac tacgggccgg gaatgcccta ttggggattc aggtgtctgt cctgcggcga
     1201 gattctcgat cccgtgattt gggagaatag aaaaaatttc cttggagcgg tgcgtgccaa
     1261 aaatctggga gcttccatcg gaaacagcgg gagggggtga agggaaatga tcgggctgaa
     1321 agaaggggat aggatgaaat accaaaggga cgggagagtt ttcgaaatag ccaaaatcac
     1381 ccgcgacttt ttggttctgc aagccctgga cgattcgact caggtcctga ccggcaaagc
     1441 gagccttgcc tttctgtttg cggagcttcc ccagtcaggg gtcccggacg tacctatgct
     1501 tccgggaaga atcggtccgc tattggcccc gatgaatagg ttggaggcac cccgctgaac
     1561 gaggtgattt gaccttggtg actcccttga aaaaaggaac tctagggtta tctcatggcc
     1621 aacgaaatcc ggccaagaaa cgattaaccc tgagggtgga caaggtcatc ggctttcacc
     1681 gagccgctta taccgacgaa cctctttaat ggtgggatca tgaaagttct tatccacacg
     1741 atggaagcag cggaatccct ggtcaagatg aagaaggcaa tcgagaccgc cctgccaaat
     1801 ataaaagggg aaaccttttc taccctggaa agcctggcac aaagactcaa ggagccctat
     1861 gacggagagg aaaaagtcgc gcttctggcg gccaccgatc agaagaatct ggaagacttt
     1921 ctttcccttc agcgcctgtt cttgaatgtt cccctgattc tcctggttcc caaccgcagc
     1981 actgagacca tggccttggc ccaccggctt cgcccccgct ttttgaacga tgtccagggc
     2041 gatttcacgg cggtgattga agtgctgcgg aagatggtcg ccgtccaagg agaaaggggg
     2101 gcggcggatt ccccccccaa atcccgcctg gggcttctct gagacagtaa agcctgtttc
     2161 tttgtttcgc ggaatggagg atatcgtgta caacaaaatt ttggttcccc tggatggatc
     2221 ggcgttggcg gagtgtatct ttccccacct tgagaccctg gccggaggat gtcaggtaaa
     2281 agaagtcgtg tttatccggg tggtcgagcc tttccggcag cccacccctt ccgaatacat
     2341 tatcaagcct gaagagataa tccggatcaa ccaggaagca aaggctgagg cggaaaaata
     2401 catcagccag gcaagcgcca aaatcaaacg ggatgggttc cgggtgaaga ccgaagtcgt
     2461 cgtcgggagt ccggccgaac tcgtggcgga ttacgccacg aagaacgggg tagacttgat
     2521 cgtcatcgcc actcacggtc gttcaggggt cagccgatgg gtctggggga gcgtggccga
     2581 ccgcatcctc cgctcggcct gcgtccccgt cctgatggtc cgggctccgg ggtgcgtgcc
     2641 cggaatctaa aagaggggcc ccaccctggt cggcgcgctt tcacaccttc atgaccttta
     2701 cgattcctcc aggaaattcc caggcaccgt cccttccatt ttcgcaatta cttacttact
     2761 cgcacgccta tcatcttgac agagagccac acaaaggata tggcccgatg gcccaactcg
     2821 ggtgcccggt gaatccggac ccgaggaaag gagaaacaag agatgaaaga gctggatccc
     2881 gcaccagggg gttggaaatc ttctcaagaa accagggtaa ccaaactgaa cggaaccttg
     2941 aagacggctt ggatggctga tttaaaaaaa gcgggagaac gggtcgaatg tctttggtcc
     3001 aaaagcaatg ccctccggca gagccggagg gctgaaaaat catgatgcgc tcaatggggt
     3061 gaaaagtggg tgtcgagcgt atcagtccgg aggaggcccg ccaggggctg gaatcaggaa
     3121 ggcttctgct ggtctgcgct tatgaggatg aaaaccgatt caggcagatg cagctggagg
     3181 gcgcgatttc cttcagggaa ttccaaacga aacttcccac ttggggcaaa ggccaggaga
     3241 tcgtctttta ctgaacctga tcccaggagg gaagcgccgc cggtcaggca gccaggtata
     3301 tcgaaatggg gtacaagaac gccaaggccc tcaaaggagg ggtgacagcc tggaaagaag
     3361 ccgggtaccc catcaagaaa gaaggtaatc catgaccccg ggttggataa ttgggtacat
     3421 cacccccatc cgacctctcc cccgtcagga gggaatttct tttctatatc aatatcttta
     3481 ccctcccccc aacaagagac tgtgtcataa tcgcccggtc gcccttcgac gggctcaggg
     3541 cgaatggcta agtggctgat aagtggctga attgtaatga gctcataatc cgttcgtggt
     3601 gagcttgtcg aaccgtgaac ggattacgac acagtctcac aaggggggag gggagagcgg
     3661 ggtagatttt tcggcctaga gggtcccatg ttggtcagca agttgggtca gcgcccgccg
     3721 aatcagggcc ttggccaacg gaactttgta atggttgtcc cgaagcggcc gggcctcctg
     3781 aagggccgat tccgcaacct cccggcagag acctgcggag aattttttcc ctcccagaat
     3841 cgattccgct tccttcgccc tccagggaat ggtcgccatt cctccgagca ccaatcgacc
     3901 ctcggtaatg gtatccccat cgaatgtcag aattgcagcc acactgacca gagcgaagga
     3961 ccaggctttc cgctccatgg ccttgagata aatcccccgc gagccccggc ctggaagagg
     4021 gactgccacc tcggtgatga gttctccggg ttcaaggatc gtcatctggc ggcgctccgg
     4081 ccgcggctct tgatacagtt cctcgagggc gatctcccgc atccctgtcg ggctggcgat
     4141 cttgattcgg ctctggaggg ccatcaaggc gggggctaga tcggaggggt gggaagcatg
     4201 gcatatggcc cttcccagga tggtgtgata atgattctcc ccccgcgcag cccagcaaaa
     4261 ttccccgcct ttgagccagc aaagggaaag gtgatttcga taataccagc aacggggacg
     4321 ctggcagagg tttccgccaa ccgtacccat gttgcgcagc tggagggttg ccgtggactt
     4381 cagggcttga tgcaggagag gccactggga aatgactgcc gggtgttctt ccaccatgga
     4441 gagcggggtt aaggccccca ggcgaaggtc cggcctcatg gagaccccct tcaaagaact
     4501 gagccgctta agatcaatca agcgatcggg tttttggatt cccctcttta actcccccag
     4561 caaatcggtc ccgccggcca gagcggcggt ccggggttcc tgcaagatcg tgagggcgga
     4621 cgccaaatcc tgagtttcga tgtgttcaaa actattcatg ggcggtctcc ttttggccct
     4681 gccgcacccc ggaatccttc ccttgtccca atgcttccag aaccttttcc cgggtgatcg
     4741 gaagctcttt gattctcact ttcaacgcat cggcaagggc attggccacg gctgccgccg
     4801 tcgggatgat cgggggttcg cccacccctt tggaccccag gttgttcgcc tggaggtcgg
     4861 gccggtcgac catatcctgc tccaatgggg gaacatcgag gcaggtgggg attttataat
     4921 cttccaggtt gccgttgacc acgatcccgg ctcgccggtc gatgaatctc ctctccgtca
     4981 gggcaaagcc aagtccctgc aagacgcctc cggtgatttg gctggagagg gtgagcggat
     5041 tgatcacccg gcccgagtcg tggacggcca cgaccttttc gatctttacc tcgccggtat
     5101 cggtgtccac ctccagctcc acaaactggg cgccgaaggt gttcacgttg gccttctcgg
     5161 gattgggctg ccgcgctccc tggccgatga tcatgaaatt cctaacgttg gagagaacat
     5221 cccggatggc cgccggcttt tgcagggcgg ggctgcgcag gaatccttcc tggatattca
     5281 ggggctcccg ggggatttcc aatacttggg cggccacatc caaaagctgg cgcttcgcgt
     5341 catgagcagc cacccggacc gccgggccga cgctgggcag ggtcatggag ccggcgctga
     5401 tcggggcata gggagccaac tgggtatccc cgagctctac cgagaccttt tccatttcca
     5461 atcccagctc ttcggcggca atctgggcca ggacggtttt ggtccccgtg cccaggtcct
     5521 gggtgccgct gatcaccgtt acggttccat ccgggttgat tttgaccagg gcataagcgg
     5581 gcggcccgcc gctccctccc cagacctggg aagccatgcc ccgccccctg cgtttgttgc
     5641 cctgcccttc cggcgggcga tcctcccagt ggatcttttg agcgcccgtg gtgtaagcct
     5701 ctcgaaggcc cttggtcgag taaggcttcg cggtcgtctg gttgagatcg gtataattct
     5761 tcagccgaag ggaaaggggg tccatcccca acttttccgc cagttcatcc atgatcgatt
     5821 ccagggtgaa ggttccctcc acatagcccg gaccccggaa ggcggagagc ggcccggtgt
     5881 tggtgaagac gaggtactgc tccgtcttca catgcgggca ggcgtaaagc tgccgcaccg
     5941 ggcccccgac cgccggagcc cccagggcat acgctccagc tgcggtgtag gcccgaagat
     6001 ggaaggctgt taatgtcccg tccttcttgg ccccggcctt taaatattgg gtgcttgccg
     6061 ggcggttccc ggcggccaga ttttcctcat gccggtcaag gagaattttg accgcccttc
     6121 ccgtccggcg ggctgcgatg gcggccagaa tcgagtattt gcccatgaag ttcttgctgc
     6181 cgaacccgcc gcccatgtat ttttttatga tccggacttt atccaggggg agattcaaga
     6241 gggctgcagc ctgcgaccga accccgaaga tatgctgggt cgaatcccag agggtcaatc
     6301 gatccccttc ccagagggct acgcttccat gggtctccag acaattatgc agggccgtct
     6361 gggtcttgaa gacctcctcg acgatgacct cggcttcccg gaaacccgtt tctaggtttc
     6421 cccgctcgta aaggtccggc gctcccttcc agaggtttcc gccgggatgg aggcgggggg
     6481 catcgggccg aagggcttcc tcggcgtcga ggacgaaagg aaggggatca tactctgcgg
     6541 caatcctctc cagggcgtcc gcacatattt cctcctcttc cgcaatgacg caggcgacct
     6601 cgtcgccggc gtaacggacg gtcgggtcga gaatataggt ttgccctccg aaaaaaggga
     6661 gcggaggaat gttttggtag gtgagcacag cccggacccc gggaaccccc tcggccttct
     6721 gggtttcaat ggccttgatt ctcccgtggg gaagggggct gcggaggact ttcccgtaga
     6781 gcatccccgg cagctgaata tcggaggtga accgggcctg gccggtcact cgttctcgcc
     6841 catcgatgcg cgttttgcct tgtcccacga acttcagtga tcttccggct tcccaggcgg
     6901 agagtccttc tccttccact acaatgtact cttcgtacaa gtgcccttcg aactcgctct
     6961 tgactttgat gacctgagac atcatgaccc tccttggcgc tgaagcttgg aagccgcctg
     7021 gacggcctgg agaatcttgg gataggcccc gcagcggcag aggttgcccg ccagcgcctc
     7081 ttcgatctcg cctggctggg ggttcgggtg ggcttccagc agggccttga cggacataat
     7141 ct
//
LOCUS       DASPRR010000539         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2067987, whole genome shotgun sequence.
ACCESSION   DASPRR010000539 DASPRR010000000
VERSION     DASPRR010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067987"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VEL68_19975"
     CDS             <1..934
                     /locus_tag="VEL68_19975"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HXZ38315.1"
                     /translation="KKKVIIDCDVGVDDALASILAFHSPELEVMGVTGVHGNVSLDKV
                     MKNIEKVLTLLRPSPRPSIARGAQRPLQGKPVHAESFHGEDGLGGAKIPVDEEEEWWE
                     VFTGSADELIAQTARRYPGEVTLIAIAPLTNLALALQRDPGSMKKLKEVVIMGGAVRT
                     KGNITPQAEFNFYVDPLAAKQVLDSGLRFTLVPLDATHQVPLTPEIMEKRVKPLRNSL
                     SRFVVEATGYESSERHFRGGRKVSYLHDPLAVGAVIDPDLVRKEKLSVSVEAKEGEFY
                     GKSYEVPQGEKNMEVCLEVNREKFLDLFLTRLKG"
     gene            939..1862
                     /gene="rbsK"
                     /locus_tag="VEL68_19980"
     CDS             939..1862
                     /gene="rbsK"
                     /locus_tag="VEL68_19980"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HXZ38316.1"
                     /translation="MQGRILVLGSSNIDLILRVPRFHGPGETISGENLVTAFGGKGAN
                     QAIAAKRLGGEVNFLTKLGRDSFGESYRKYLTDKGLPAGFLLQDKKLPTGVAVIELIP
                     KGENRIIVSPGANASLSVKDLLAHPDVWRAAGVFVAQLETPLNTVKKGLNLGRKMGCL
                     TILNPAPAAPLSPEILSLVDFLVPNESEAQSLSGIRIRGKKDLPRIAKTLLEKGVKNV
                     VVTRGAQGLYFKNQDKEIWMDAFRVKAVDTTAAGDAFMGALACGLAGGKPTREALRQA
                     NAAGALATTKLGAQPSLPHRREVERFLERTP"
     gene            2052..>2805
                     /locus_tag="VEL68_19985"
     CDS             2052..>2805
                     /locus_tag="VEL68_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXZ38317.1"
                     /translation="MAKTGEPQGDTARAMEEKAEGRLRELFEKMPYPASIFLFTDEGQ
                     NEPFNQAAREVLHILHRVSPKIQIKEYDLGHEQARKWKIQSSPTLLFDPDRCKARWLG
                     APIGEETKTLIIALQMLGYRTTGLSEDSRKILKKITSPRHIKVFVSPTCPYCPQQAVN
                     ALNAAFERPDLISLEIIDIQVNPDLAQKYGAQSVPQVFANEKLIAMGAQPEELFMSSL
                     EKMEEQRIYIPESDAAELETDLVIVGGGPAGLT"
BASE COUNT          680 a          757 c          794 g          574 t
ORIGIN      
        1 gaagaagaaa gttatcattg actgtgatgt gggggtggat gatgccctgg catcgatcct
       61 tgcttttcat tcacccgagc tggaggtgat gggggtcacc ggggtccatg ggaatgtttc
      121 cctggacaag gtaatgaaaa atatcgaaaa ggttctgact ctcctcaggc cttccccacg
      181 gccctcgata gcccgggggg cccaacgccc tttacagggg aaaccggtgc acgccgaatc
      241 ctttcatggg gaagacggat tggggggcgc aaaaattcca gtggatgaag aggaagaatg
      301 gtgggaggtt ttcaccgggt cggcggatga actcatcgct caaacggccc gccggtatcc
      361 cggagaggtc accttgattg ccatcgcccc cctaaccaat ttggccctcg ccctgcagag
      421 ggatcccggc agcatgaaaa aattgaagga agtggtgatc atggggggag cggtccggac
      481 gaaggggaat atcacccctc aggcggagtt caacttctac gtcgaccccc tggccgccaa
      541 acaagtgctg gattccgggc tgcgctttac cctcgttccc ctggatgcta cccaccaggt
      601 tcccttgacc cccgagatca tggaaaagag agtgaaacct ctccggaatt cgctttcccg
      661 attcgtggtc gaagccacgg gttacgagtc cagcgaaagg cattttcggg gcgggaggaa
      721 agtgtcctat cttcacgacc cccttgccgt aggagcagta atcgatccgg acctagtgag
      781 aaaagaaaag ctttcggtat ctgttgaagc gaaagagggc gaattctacg ggaaaagcta
      841 tgaagtcccc caaggagaga aaaatatgga ggtttgcctg gaagtgaacc gggaaaaatt
      901 tctggatttg tttttaaccc ggctgaaagg ctgagcccat gcaaggtcgg atccttgtcc
      961 taggcagttc gaatatcgat ctcatcctcc gtgtcccccg cttccatggc cccggggaga
     1021 cgatcagcgg agaaaacctg gtgactgcct tcggcgggaa aggggccaac caggctattg
     1081 ccgccaagag gctgggagga gaggtcaatt ttctcaccaa actggggagg gattcattcg
     1141 gcgaatcata ccggaaatat ttaaccgaca aaggcctacc cgccggtttt ctccttcaag
     1201 acaagaaact ccccacggga gttgcggtca tcgagttgat ccccaaggga gaaaaccgaa
     1261 tcatcgtctc gccgggagcc aatgcatctc tatcggtaaa agacctgcta gcgcatcctg
     1321 atgtgtggag ggcagccggt gtctttgtcg ctcagctgga aacccctctg aatacagtga
     1381 agaaaggcct caatctgggc agaaaaatgg gctgtctcac gatcctgaac ccggctccgg
     1441 cagcccccct ttccccggaa attctctccc tggttgactt tttagttccc aacgaatcgg
     1501 aggctcagtc cctgagcggg atcagaataa ggggaaagaa agacctgccc cgaatcgcca
     1561 agaccctgct ggaaaagggc gtcaagaatg tggtggtcac gcggggagcg cagggtctgt
     1621 atttcaagaa tcaggacaaa gaaatctgga tggatgcctt ccgggtgaag gccgtggata
     1681 ccacggcggc aggagacgct ttcatgggcg ctctggcctg cggccttgcg ggggggaagc
     1741 cgacccgcga ggcgttgagg caggccaatg ccgcgggagc cctggccacc accaaactgg
     1801 gcgctcaacc ttcgctccct catcgaagag aggtagagag gtttttggaa agaacccctt
     1861 gaatcgtctg atcgattacg attttcatct tggccgattg tccttccgcg accccctgaa
     1921 tcttttgagc ggcctccttg gccgcttttt ctttctaacc ttctagagga tggatcaccg
     1981 cacaggaaag aagtcttttt ctatcaggtc gctggtataa taatagagga taacaagcca
     2041 gggaggagaa gatggccaag acaggggaac cccagggaga taccgccaga gcgatggagg
     2101 aaaaggccga aggccgcctt cgggagcttt ttgaaaagat gccctacccg gcatcgattt
     2161 ttcttttcac cgacgagggt cagaacgaac ccttcaatca ggctgcgcgg gaggtcctcc
     2221 acatcctgca ccgggtatcc ccgaaaattc aaatcaagga atatgacctg ggccacgaac
     2281 aggcccgtaa atggaagata cagagttcac cgactcttct cttcgatccc gatcgatgca
     2341 aagcccgctg gctgggagct cctatcggtg aggaaaccaa gaccctgatc atcgcccttc
     2401 agatgctggg ttatcgaacc acgggactca gcgaagattc ccgcaagatt ttgaaaaaga
     2461 tcacttcccc ccggcacatc aaggttttcg tgagccctac ctgcccttac tgcccccagc
     2521 aggccgtgaa tgctctgaat gccgcctttg aaagaccgga tttgatctcc ctggagatta
     2581 tcgacattca ggtcaacccc gacctggccc agaaatacgg cgctcagagc gttccccagg
     2641 tctttgccaa tgaaaagctc atcgccatgg gggcccagcc ggaagagctc ttcatgtctt
     2701 cgctggaaaa aatggaagag cagagaatct acatccccga gagcgacgcc gcggaactag
     2761 aaacggattt ggtcatcgtt gggggaggac ccgccggtct caccg
//
LOCUS       DASPRR010000540         4534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_277810, whole genome shotgun sequence.
ACCESSION   DASPRR010000540 DASPRR010000000
VERSION     DASPRR010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277810"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VEL68_19990"
     CDS             <1..415
                     /locus_tag="VEL68_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ38318.1"
                     /translation="DGKCVVKADRANEYIRKMREAEGILLGSPTYVADITANLKAILE
                     RATIVCRANGGEMFKRKPGAGIVAVRRAGAIQAINVLNAFFLINQMIVVGSSYWNVGI
                     GRNLGEVNSDLEGIQTMKALGQNMAWLLKKIHSQG"
     gene            complement(485..1123)
                     /locus_tag="VEL68_19995"
     CDS             complement(485..1123)
                     /locus_tag="VEL68_19995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXZ38319.1"
                     /translation="MNKTVTIILGVLVAILVLAGVILYPEYTKQKGALQTCEKKLADI
                     EKKGPALSEAQLKSACDLLVSSLNDQIKKQEITIKEFQGGISLSLVDRILFRLGEATL
                     TPQGQKVLAKVGEGLKKIEGKMIQVVGHTDTIPIRREFLDKFPSNWELSAARASTVVR
                     YFQDKSGLDPKGMEAVGRSFYQPAAANDTDKNRALNRRVEILIAPVMELKEK"
     gene            complement(1363..4215)
                     /locus_tag="VEL68_20000"
     CDS             complement(1363..4215)
                     /locus_tag="VEL68_20000"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HXZ38320.1"
                     /translation="MMAEGKIEAIATDLKRIVEGNILSDELSRTIYSSGACIYSLRPM
                     VIVQPRHKEDVVQCVRYAAKNGIPLTARGGGTGRAGQGIGEGIILDFIKYMNQVLEVD
                     PEKKWVRVQPGLILGRLNAFLKPMKKFFPIDPSTTDYCALGGMIANNSSGPHAIKYGA
                     ARDWVESLEVVLSSGEIIKTGPRNVGALESSGRTDGLEEKLYPSVLELLGRYQGPLQE
                     EKPDTMKNSCGYELWQVRKNGSLDLTPLLVGSEGTLGIFTEAKLRLADLGGKTHTALI
                     YFNTLDTVGKATEEILKLSPTMLEIMEKQIIDLARGKYKEMKPYLPEGIEATLFVEFE
                     GRSEEELKGKMRQVEQRLIQEEKLAVSLKAAQDTRDKEMLTKVRSVSGPILNKVKGPK
                     KPIAFIEDAAVHPRRLGEYIAGLRGILKRNGAEASIYGHAGDGNLHVMVFLDLKKTED
                     VARMLRITDESCDLVLRLKGTISGEHGDGLLRTAFVPRQYPKLYPAFVELKKLFDPEN
                     VLNPGRVVGNDPDLMGKHLKFGSEYCHVPTGSAFDGEALGAEVEACHGCGKCRSYCPL
                     AMATNDESYLGRAKASLLRELMSGNLDPDTFLASDELKRVMDGCVNCKRCLTECPTGV
                     DIPWVSLMARAHHVKNRGQSFKDRFIADTHLACRVGNTLAPLVNLAFSVGPARALMES
                     AIGLDRRRKMPPFSSKTFREMYKGSEKGNGKAAYFLSCYANYNDPEGEGRALVEVLER
                     NGYGVEVPDFECCGIARISSGGLKPALKGVGENLEKISSLVEKGIPVVFSEPSCALAV
                     KQEYPRILNSEKSAKAAAGCYEVHGFLSNLKQEGKLNTRFGEIKMKVGYHNPCHLRAL
                     GISKEVVDLLKLIPGLEVQAFSDRCCGLAGTFGMKKQNYDLSMEIGQPLFEEIKKSGV
                     DRVVTGCGSCAMQIAQGTGLKVAHPLTLLAEAYRKG"
     gene            complement(4303..>4534)
                     /locus_tag="VEL68_20005"
     CDS             complement(4303..>4534)
                     /locus_tag="VEL68_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXZ38321.1"
                     /translation="KVRVFNDRGRMVRKAKVTDRIMPGVVSLEAGSWYDPDEKGVDRG
                     GCVNVLTVDKKSPAGAFPCNSCLVEVEGEENP"
BASE COUNT          893 a         1376 c         1219 g         1046 t
ORIGIN      
        1 agacggaaag tgcgtcgtca aagctgatcg ggcgaacgaa tacatccgga agatgcggga
       61 ggctgagggg atccttttgg gctctcccac ttacgtcgcc gacatcaccg cgaacctcaa
      121 ggctatcctg gagagggcca ccattgtatg ccgggccaac ggcggggaga tgttcaaaag
      181 gaaaccagga gcgggcattg tggccgtccg ccgggccggc gccattcagg cgatcaacgt
      241 tctgaatgct ttctttctca tcaaccagat gatcgttgtg ggttcgagtt actggaacgt
      301 gggaattggc cggaacctgg gggaagtcaa cagcgatctg gaaggcattc aaaccatgaa
      361 ggccctgggt cagaacatgg cctggctgct caaaaaaatc cacagccaag gctgagtctt
      421 gaaggtcacg gcccccccat cccgcaccct atggcgcaag acggaggggt gtgcggtatg
      481 cctcttattt ttccttcaat tccatcacgg gggcgatgag gatctccacc cgccggttca
      541 aggccctgtt cttgtcggtg tcgttggcag ccgccggctg gtagaaggag cgccccaccg
      601 cttccatccc tttcgggtct aacccgctct tatcctgaaa ataccgcacc acggtggacg
      661 cccgggcggc cgaaagctcc cagttcgagg ggaacttgtc caggaattcc cggcggatgg
      721 gaatggtgtc tgtgtgtccg acgacctgga tcatcttccc ttcgatcttt ttcagcccct
      781 ccccgacttt ggccagtacc ttttgcccct ggggggtaag cgtcgcctcc cccagtctga
      841 agaggatccg atcgaccaga gatagagaaa tgccgccctg aaattccttg atggtgatct
      901 cctgcttctt gatctggtca tttaggctgg aaacgaggag gtcgcaggcg ctcttcagtt
      961 gggcttcaga gagcgccggc cctttcttct ctatgtcggc aagtttcttt tcgcaggtct
     1021 ggagggcccc tttctgtttg gtgtattcgg gatagagaat cactccggcg aggaccagga
     1081 tcgctaccag aacgcccaga ataatcgtaa cagtcttatt cattttctcc tcccttttga
     1141 aaaagtggaa tcaatcatga atccggtctt tcgacactca ggatggagcg ggaaaagtat
     1201 aacgcctata gtatatcagc ttgacgccgg gagtttcaac ctccaaatca tgacctccgg
     1261 cagagccggg ggtttatcat atcaaaatta ggcataaggg cgacgtgtcg agctgaaggg
     1321 agcagccttc tcagccgaaa ccaaatgaaa cccaaaagaa gcttagccct tgcgatacgc
     1381 ttcagccaga agagtcaatg gatgagctac cttcagcccc gtcccttggg cgatctgcat
     1441 ggcgcaggag ccgcagccgg tgactacccg gtctacgccg cttttcttga tttcctcaaa
     1501 caggggttgg ccgatctcca tggagaggtc gtagttctgc ttcttcatgc cgaaggtgcc
     1561 ggccaggccg cagcatcggt cgctgaaagc ctgtacctca agacccggaa tcagctttag
     1621 gaggtccacc acttctttgg aaattcccag cgcccgcagg tgacaggggt tgtggtagcc
     1681 cactttcatt tttatttccc cgaatctggt attgagcttc ccctcctgtt tcaggttgga
     1741 cagaaatccg tggacttcat agcagccggc agccgccttg gcggattttt cagagttgag
     1801 gattcgagga tattcctgct tcactgctag ggcgcagctc ggctcgctga agaccacggg
     1861 aatccccttc tccaccaggg aggaaatctt ctccaggttc tcccctactc ctttgagggc
     1921 cggcttcagt ccccccgaag aaatccgggc aatcccgcag cactcgaaat ctggaacctc
     1981 caccccgtag ccgttccgct ccagcacctc caccaaggcc ctgccttctc cttccggatc
     2041 gttgtagttg gcatagcagc ttaggaagta agccgctttt ccgtttccct tttccgatcc
     2101 tttgtacatc tcccgaaaag tcttggagga aaagggaggc atctttcttc gtcggtccaa
     2161 gccgatggcg gattccatca gagcccgcgc gggccccacg gaaaaggcaa ggttgaccaa
     2221 gggggcgagc gtgtttccga cccggcaggc cagatgggtg tcggcgatga accggtcctt
     2281 gaaagactgc cccctgtttt tcacatgatg ggcccgggcc atgagcgaaa cccaggggat
     2341 atctacgccg gtggggcact cggtgaggca ccgtttgcag ttcacgcacc catccatcac
     2401 ccgtttcaat tcgtcggagg ccaggaaggt gtcggggtcc aggtttcccg acatcagctc
     2461 ccgcagaagg ctggctttgg cccggcccaa ataggattca tcgttcgtgg ccatggccag
     2521 cgggcaatag gaccggcact tgccgcagcc gtggcaggct tccacctccg cgcccagggc
     2581 ctccccgtcg aaagcactgc cggtgggtac atggcagtat tcggagccga acttcagatg
     2641 tttgcccatc aggtcggggt catttcccac cacccttccc ggattcaaaa cattctccgg
     2701 gtcgaagagc ttcttcagct ccacaaaggc ggggtacagc ttggggtact gccgcggcac
     2761 gaaggccgtt cgcaggagcc catccccgtg ttccccgctg atggttcctt tgaggcgcag
     2821 caccagatcg cagctttcat cggtaattct cagcatccgc gccacgtcct cggttttttt
     2881 caggtccaga aagaccatga cgtgaagatt cccgtccccg gcgtggccgt aaatactggc
     2941 ctccgctcca ttgcgtttta aaatcccccg cagcccggcg atatactcgc ccaaacggcg
     3001 gggatggacc gcagcatcct cgatgaaggc aatgggtttc ttgggccctt tcactttgtt
     3061 gaggatgggg ccggagacgc ttctcacctt ggtgagcatc tccttgtccc tggtgtcctg
     3121 ggcggccttg agggagacgg ccagcttttc ctcctggatc agccgctgct ccacctggcg
     3181 catcttcccc ttgagttctt cctccgaccg cccttcaaac tccacgaaga gggtcgcctc
     3241 gatcccttcg ggcaggtacg gtttcatctc tttgtatttg cccctggcca ggtcgatgat
     3301 ctgcttttcc atgatctcca gcatggtggg agaaagcttc agtatctcct ccgttgcctt
     3361 ccccacggtg tccagggtat tgaaatagat cagcgccgta tgggtcttgc cgcccaggtc
     3421 ggccaggcgg agtttggctt cggtgaagat tcccagcgtt ccttcggagc ccaccagaag
     3481 gggggtgagg tccagggaac cgtttttgcg aacctgccaa agttcgtagc cgcaggaatt
     3541 cttcatggtg tcgggcttct cttcctggag gggcccctga taacgtccca gaagctccaa
     3601 gacgcttggg tagagtttct cctccaaccc atcggtcctg ccggaagact caagggcacc
     3661 cacgtttcga ggcccggtct tgatgatctc tccgctcgag aggaccacct ccagggactc
     3721 cacccaatca cgggccgccc cgtacttgat ggcatggggg ccgctggagt tgttggcgat
     3781 cataccgccg agagcgcagt aatcggtggt cgaagggtcg atgggaaaga atttcttcat
     3841 gggtttcaag aaggcgttca gccttcccag aatgaggccg ggctgcaccc gcacccactt
     3901 cttctccggg tcgacctcca ggacctgatt catgtatttt atgaaatcca gtatgatccc
     3961 ctcaccgatc ccctgccctg cccggcccgt gcccccacca cgggcagtta gagggatccc
     4021 gttcttggcc gcgtacctaa cacactggac cacatcttct ttatgtcggg gctgaacaat
     4081 caccatgggg cgcagggaat agatgcaggc cccactgctg tatatcgtcc ggctcaactc
     4141 atcggagaga atgtttcctt ccacaattct cttcaaatcg gtagcgatcg cttcaatctt
     4201 gccttcggcc atcatcgatc tccttcgacc cttattcgtc ccggcaatcc atcctactat
     4261 tttccccctg ttcccattca tgatttaggt cagtggagaa aatcaaggat tttcttcgcc
     4321 ctccacctcc accagacagc tgttgcaggg gaacgctcca gcgggggatt tcttatccac
     4381 tgtgagaacg ttgacgcagc cgccccggtc cactcccttc tcgtcagggt cataccaaga
     4441 gccggcctcc aggctcacca ccccgggcat gatcctgtcc gtcactttcg ctttcctcac
     4501 catccgaccc cgatcgttga ataccctaac cttc
//
LOCUS       DASPRR010000541         5704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_134724, whole genome shotgun sequence.
ACCESSION   DASPRR010000541 DASPRR010000000
VERSION     DASPRR010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134724"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VEL68_20010"
     CDS             complement(<1..713)
                     /locus_tag="VEL68_20010"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HXZ38322.1"
                     /translation="MIVDLAFTPHELEKKELTGKSVVVIDALRATTTMTVGFENGAAA
                     FIPVETVEEARDLVAKNPGFLLAGERGARPLEGFHLGNSPRDYRPQKVKGKTIVMTTT
                     NGTRALVVARGGAEVLIGSFLNLSALVDRLIEAERDVIIACSGEKDIFCLEDTVCGGA
                     IVERLEKTGLPLVKTDAAGAAKILYEQCEGNVYGMLYTCEWGVYLEGLGLGKDVRVCA
                     QVDSSKIVPVYRDGRIILDR"
     gene            complement(802..3477)
                     /gene="polA"
                     /locus_tag="VEL68_20015"
     CDS             complement(802..3477)
                     /gene="polA"
                     /locus_tag="VEL68_20015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXZ38323.1"
                     /translation="MSAKMPKVFLIDGSSYIYRAFFAIGHLSNSKGFPTNAIYGFTNM
                     LLKVLRDRGAEYAALAFDAPGPTFRHEVFGEYKANRPSMPEDLRIQIPFIKEIVSALR
                     LPILEKEGYEADDLIATLARKLEREGTETVIVSGDKDLMQLISSHVTMYDPMKDKTFQ
                     VPEVRERFGVSPEKVVEVMGLMGDTSDNIPGVPGIGPKTAGQLIEEFGSIAELLKNID
                     RVKNAKVRASLDRYAEQARLSRELATLDSDAPLDWTLENLRRGEPDRKKLQEIFKEME
                     FSKLLKDFSLRPDHRDDDYRPISSQRELEGLVRNLRKAGAFALDLESTSIEPMRAEIV
                     GLSFSFQPHQAFYLPVGHTYEGAPEQLSRDEVLRILRPLLEDPGLKKYGQNIKYDCIL
                     LAKSGVRLKGIAGDNMIASYLLNPSKHRHSLEELSREYLDRQIITYEDVAGAGAKAIP
                     FNQVEVEKACRYSCEDADLTLILASLLIPKIETDGFAELFGNVELPLIEVLAVMEMNG
                     VKLDLPLLAVMSDEFEKQMEKISGEVYDLASGEFNINSPQQLGKVLFEKLKLPGGRRT
                     KTGYSTDVDVLTELAKDFPLPAKVLEYRSLSKLKSTYVDALPQLVNPATGRVHTSFNQ
                     TVTATGRLSSSDPNLQNIPIRSPEGRRIREAFIPEAGSLILSADYSQIELRILAHLSC
                     DTFLIETFQRGEDIHAATASEIFHVPPEQVTPEMRRLAKVINFGIIYGMSAFGLAKEL
                     GIPPSVAQAYIANYFQKYHEVRDYIDKSLEQARERGYVTTLMNRRRYLPDIQNSNRSI
                     RQFAERIAINAPIQGTAADLIKVAMINIHRRFLEERRKTKLILQIHDELVFEVPEGEL
                     EEIKALVREEMEGVVKLYIPLKVDIGVGKNWAEAH"
     gene            complement(3702..4205)
                     /locus_tag="VEL68_20020"
     CDS             complement(3702..4205)
                     /locus_tag="VEL68_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HXZ38324.1"
                     /translation="MKKGHSSSVLRCAPKKISLDREDRLILNQIQRNFPVTHRPFLAL
                     ARRTGMKEKEVLERVQRLKDLGIIRRIGASFSAGAVGFSSTLCAARVPADKIEEFVSV
                     VNSYPGVTHNYEREGEYNLWFTLIAPSRGKIGEILEEISRKTGVREILNLPATKTFKI
                     AVDFNFD"
     gene            complement(4227..4958)
                     /locus_tag="VEL68_20025"
     CDS             complement(4227..4958)
                     /locus_tag="VEL68_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38325.1"
                     /translation="MKKILVVFLLLWALPVAASDISFDRTYTQQNFRDFSEEIGSVLL
                     YRSIGPAVSLGITGFNLGVEIMASDISENKDFWKKAVSDGNPPSYLVNSRISFTKGLP
                     LDFDVGVVLGRILDPDVPYFGGEVRYAIVKDRGLIPGVAIRGSYSQTFNVDQLDLRTY
                     GLDLSVSKGFGVGIKIIPYAGLGASWVSSKASHLTSGLTLNKEEFYKTHGFLGARLEM
                     GLVAITAEVDYGVIPSYNLQVGIAF"
     gene            complement(5013..>5704)
                     /locus_tag="VEL68_20030"
     CDS             complement(5013..>5704)
                     /locus_tag="VEL68_20030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ38326.1"
                     /translation="PENVQFYLRQAIPHGYFQTKFISPNAFSYGSKTPGEPDLEKLEN
                     LLAHCQGTGLEGIHFGCFPSEVRPDWVIPGVLELVKKYCRNRTIVLGAQSGSDPLLEK
                     LKRGHTADQALNAARWIRQAGFRPHVDFVFGFPGETMEDRKLTLQLIDKMVQGLGAKV
                     HAHTYMPLPGTPLFRERPSVLDRATKETLLDWEKKKKLDGWWKEQENIAWKIVEWKDR
                     GIIQGRDEPSP"
BASE COUNT         1242 a         1647 c         1499 g         1316 t
ORIGIN      
        1 cgatcaagga tgattctccc atcccggtaa acggggacga tcttggaaga atcgacttgg
       61 gcgcaaaccc gcacatcctt acccagcccg agcccttcca gatatacccc ccactcgcag
      121 gtgtagagca tcccatacac attcccttcg cactgctcgt acaaaatctt ggccgcgccg
      181 gctgcgtccg ttttgaccaa gggaaggccg gtcttttcca gcctttccac gatggccccg
      241 ccgcagaccg tatcctccag gcagaagatg tctttctccc ccgagcaggc gatgatcaca
      301 tcccgttccg cctcgatcaa acgatcgaca agggcgctta agttcaaaaa ggacccaatg
      361 agaacctccg ctcccccccg ggcaacgacc aacgcccgtg tcccattggt agtggtcatg
      421 acaatggtct ttccctttac tttctggggc cgatagtcgc ggggggaatt ccctaaatga
      481 aaaccttcca ggggtcgggc tcccctctcc ccggctagaa gaaagccagg attcttggcc
      541 accaggtcgc gagcctcttc gaccgtttct acggggataa aggccgctgc cccgttctca
      601 aaaccgaccg tcatggtcgt ggtcgcccga agggcgtcga tcaccacaac ggacttgcca
      661 gtcagctctt tcttttccaa ttcatgcgga gtaaatgcca aatcaacaat cataaggagg
      721 ctttcagctt tcagcgctca gctttcagct tcaagcggtc cccttcttgc tgaccgctga
      781 aagctgattg ttgatcgctg cttagtgggc ttccgcccag ttcttcccca cgccgatgtc
      841 caccttcaaa ggtatgtaaa gcttcacgac cccttccatc tcctctctca ccagggcctt
      901 aatttcctcc agctctcctt cggggacttc gaagacaagc tcgtcatgga tctgaaggat
      961 gagtttggtc ttgcgccgtt cttccaaaaa tctccgatga atgttgatca tggctacttt
     1021 gatcaagtcc gccgccgttc cctggatggg agcgttgatg gcgatgcgct cggcaaactg
     1081 gcggatactg cggttactgt tctggatgtc cggcaggtat ctccgccgat tcatcagggt
     1141 ggtcacatat ccccgttccc tggcctgttc cagggattta tcgatgtaat cccgcacctc
     1201 gtggtacttc tggaagtagt tggcgatgta agcctgggcc acgctcgggg gaatgcccag
     1261 ctccttcgcc agtccaaaag cactcatccc gtaaataatg ccgaaattga tgaccttggc
     1321 caggcggcgc atctccggcg ttacctgctc cggcggaaca tgaaaaatct ccgaggcggt
     1381 ggcggcatga atgtcttccc ccctctgaaa ggtctcgatc aaaaaagtgt cgcaggagag
     1441 atgggcaagg atccgcagct cgatctggga atagtccgcg gaaaggatca aagagcctgc
     1501 ttcgggtatg aaggcctccc gaattcgccg gccttcgggg gaacggatgg gaatgttctg
     1561 caggttggga tcgctgctgg agagtcgccc cgtggcggtg acagtttggt tgaaagaggt
     1621 atggacccgc ccggttgcgg gattgaccag ttggggaagg gcatccacat aggtcgattt
     1681 gagcttagac aggctccggt attcgagaac tttggccggc aggggaaaat ctttcgccag
     1741 ttcggtgaga acatccacat cggtggagta accggtcttg gtacgtctcc cccccggaag
     1801 tttgagtttt tcgaaaagga cctttcccag ctgctgcgga gaattgatat taaactcccc
     1861 gctggccagg tcatagactt cccccgagat tttctccatt tgtttctcga attcgtcgga
     1921 catcactgcc aggaggggca aatcaagctt gaccccgttc atttccatca ccgctaggac
     1981 ttcgatcaaa ggaagctcga cattcccaaa gagctccgcg aacccgtccg tctcgatttt
     2041 ggggatcagc aggcttgcca ggatcagggt taggtcggca tcctcgcacg aataccggca
     2101 agccttctcc acctctacct gattgaaggg gatcgccttg gctccggctc cggccacgtc
     2161 ttcataggtg atgatctgcc ggtccaggta ttcgcgggac agctcttcca ggctatggcg
     2221 gtgtttggag gggttcaaaa gataagaggc gatcatgttg tcgccggcga ttcccttgag
     2281 gcggaccccc gatttagcca ggaggatgca atcgtacttg atattctgcc cgtatttctt
     2341 cagccccggg tcttccagca gggggcggag aatccggagc acctcgtccc gggagagctg
     2401 ctcgggagcc ccctcatacg tatggccaac cggaaggtaa aaagcctgat gaggctgaaa
     2461 agaaaaagaa agacccacga tctccgccct catgggttca atggaggtgg actccaggtc
     2521 cagggcaaat gcccccgcct ttcgcaagtt ccggaccaac ccttccaatt cccgctggct
     2581 gctgatcggc cgatagtcgt cgtcccgatg gtcgggccgc agggaaaagt cttttaacag
     2641 cttggaaaac tccatttcct taaagatctc ttggagcttc ttccggtccg gctcccccct
     2701 cctcaagttt tccagggtcc aatccagcgg ggcatccgag tccagagtgg ccagttcccg
     2761 gctcagcctg gcttgctcgg cataccggtc caggctggcc cggaccttgg cgttcttgac
     2821 tctgtctata ttcttcaaga gctccgcgat ggacccgaac tcttcgatga gctggcccgc
     2881 ggtcttgggc ccgatccccg gaacccctgg gatgttgtcc gaggtatccc ccatgagccc
     2941 cataacctcg accacctttt ccggcgaaac cccaaagcgc tcccttactt cgggaacctg
     3001 aaaggtcttg tctttcatgg ggtcgtacat ggtcacgtgg gaactgatga gctgcatcag
     3061 gtccttatct ccggatacga tcaccgtttc cgtcccttcc ctctccagct tcctggccag
     3121 ggtggcgatg aggtcatcgg cctcgtaccc ttctttttcc agaatcggca gccgcagggc
     3181 ggaaacaatc tccttgatga agggaatttg aatccgaagg tcctcgggca tggaaggccg
     3241 gttggctttg tattccccga agacttcgtg gcggaaggtg ggacccgggg cgtcaaaggc
     3301 aagggccgcg tattcggctc ccctgtctct taggaccttc agcagcatgt tcgtgaaacc
     3361 atagatggca ttcgtgggaa agcccttgga gttggataaa tggccgatgg caaaaaaggc
     3421 ccggtagata taggagctgc catcgatcag gaaaaccttt ggcatcttcg cggacatggt
     3481 atgagtatgc cataagcccc ggggaaagga aaggtatgag gcgtgagaca tgaggttgga
     3541 ggcttggacc ttggggcatg aggttcaagg atcagacttc gaacagccaa aaccagcaaa
     3601 gattgaccag caacagaatc ttaccaccgt attatgggtg cctacctcgc ggcatttgct
     3661 taagctgagc gctgaaggct gatcgctgat tgcagccttt atcaatcgaa attaaaatcc
     3721 accgcaatct tgaaggtctt ggtcgcgggc aggttcagaa tctcccgaac tccggtcttg
     3781 cgggagattt cctccaggat ctccccgatc ttcccccggg aaggagcgat gagcgtaaac
     3841 cagaggttgt actccccctc cctttcataa ttgtgggtca ctccggggta ggagttcacc
     3901 acggatacaa actcctctat tttatccgca ggcaccctgg cggcgcacaa ggtggagctg
     3961 aaacccacgg cgccggcgct gaagctggcg ccgatgcgcc ggataatccc caggtccttg
     4021 agcctttgaa ctcgctccag aacctctttc tctttcattc ccgttcttcg tgccagagcc
     4081 agaaagggcc gatgggtcac gggaaaattg cgctggatct ggttaagaat caatcgatcc
     4141 tcccgatcca gagaaatctt tttcggagcg cagcgcagaa cagagctcga gtggcctttc
     4201 ttcactgtaa gacaacccct gcttccttaa aaagcaatcc caacctgcaa attgtaggag
     4261 ggaatgaccc cataatccac ttcggcggtg atcgccacaa gtcccatctc cagcctcgct
     4321 cccaggaatc catgggtctt gtagaattct tctttgttca gggtgaggcc tgatgtgaga
     4381 tggctggcct tgctcgaaac ccatgaagcg cccagaccag cataagggat aatcttaatc
     4441 cctaccccga atcccttgct gaccgaaagg tccagtccat aggtccgcag atcaagttga
     4501 tccacattga aggtctggga atagctgccc cgaatggcta cgccgggaat gaggcctcga
     4561 tctttcacga tggcataccg tacctcgccc ccaaagtagg gaacatccgg gtccaggatt
     4621 cttcctagca caactcccac gtcaaaatcc aagggaagtc ctttggtaaa gctgatccgg
     4681 gagttgacga gatacgaagg agggtttccg tcgctaaccg ctttcttcca gaaatcttta
     4741 ttttcgctta tatcggaagc catgatttcc acccccaggt taaaccccgt gatccccaaa
     4801 gaaacggccg gcccgatgga ccgatagaga aggacagacc cgatttcctc gctgaaatcg
     4861 cgaaagttct gctgggtata agttcggtca aaagaaatgt cggaagccgc taccggaagc
     4921 gcccatagga gcaggaaaac cactaagatt ttcttcatag cttcctcctt tggccaaaga
     4981 ctgtcctttt ctagcaaatc gccttccgga attcaagggg aaggctcgtc tcttccttga
     5041 atgatccccc tatccttcca ctccacgatc ttccaggcta tattctcctg ttccttccac
     5101 cacccatcca actttttctt cttttcccaa tccaggaggg tttctttggt agcccgatcc
     5161 agaacagatg ggcgctcccg gaaaaggggg gttcccggaa gaggcatgta ggtgtgggcg
     5221 tggaccttgg cccccagccc ctgaaccatt ttgtcgatca attgaagcgt gagctttcta
     5281 tcttccatcg tctccccggg aaacccaaat acgaagtcca cgtgaggcct gaagccggcc
     5341 tggcgaatcc agcgggcggc attcagggcc tgatctgccg tatggccccg tttcagcttt
     5401 tccagaaggg gatcactgcc cgattgagcg cccagaacga tggttctgtt ccggcaatat
     5461 ttcttcacca attccaggac ccccggaatg acccaatctg gccggacttc ggaggggaaa
     5521 caaccgaaat gaatcccttc cagccccgtt ccctgacagt gggctaacag gttttccaat
     5581 ttttcaaggt cgggttcacc gggagtcttc gagccgtagg aaaaggcgtt gggagagatg
     5641 aacttggtct ggaagtaccc atggggaatg gcctgccgta gatagaattg cacgttttcc
     5701 ggtt
//
LOCUS       DASPRR010000542         6680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_448171, whole genome shotgun sequence.
ACCESSION   DASPRR010000542 DASPRR010000000
VERSION     DASPRR010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448171"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..2329
                     /locus_tag="VEL68_20035"
     CDS             377..2329
                     /locus_tag="VEL68_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ38327.1"
                     /translation="MSTSKLRYLFDPLRIGAMHVKNRLVMAPMGTNLASLEGKVTPSL
                     VSYYQARARGGVGLIITDDTTITSSAIYHPHGLRLDQDPLIQSWKELTQAVHVYGAKI
                     APQLVHPSFNAPSVFSGVQPVAASPIPSRRFREIPRELTVEEIKEIVRQFGEASRRAQ
                     EAECDAVQIHCAHMHHLLGGFLSPLYNKRTDTYGGSLEGRLRLPLEVIQHIRSKVGPG
                     FPILVRISGDEFLPGGQSLEESKQIASLLVEGGVDAIQVSAGTGNNFWVVVPPTGSPE
                     APNAFLAEQIKKEVNVPIICVGRIHHPWAAENLLKSGKADMVAMGRALVADPELPNKA
                     ARGDWDEIAPCVGDSMCLTYVQMEERIRCLVNPMAGREEEDPIPPAAIAKNVLVIGGG
                     PAGMEAARVAAMRGHRVTLIERASKLGGQLLVASFPPMKQEYTCLIQYLSHQVKKAGV
                     KVELNREADLALVSSLRPETIIVATGAVPFIPRDIPGFNRECVLTAWEVLAGRSLVGP
                     KVVVVGGGKVGCETADFIAHPADDLTPKGNQVTIIELQENIALDEKSMARSLLIRRLK
                     EKGVGIILGGKVIEILPDGVKYVKEGREEALRGMNHVVLAIGAVSENTWAQRITGIPT
                     FVIGDAKHPRNALESIAEGWEIGRKI"
     gene            2448..3683
                     /locus_tag="VEL68_20040"
     CDS             2448..3683
                     /locus_tag="VEL68_20040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38328.1"
                     /translation="MKGRGKVFVWGMVLTLVFTLLATQSIVFGFTDPVRIGTIFPRSG
                     FLADLGIESWRGAEIARLERNEKGGIKGQKVIYVDTDAPNVDAAVSNAESLINREKVK
                     VICGTYWSSGGYAASAVAEKNKTLYFEFTSVSDELTQRGFKYLLRTAMLTSGMGGAIV
                     DFIVDSAKSGKIGVKLQDLKVAVIHEDSLMGTSISQFIMDDCKKKGLNVVHYEAYSAK
                     ATDLSPLVLKLKDKQTDILISACYLTDAILLSRQMKELGLKVKAQFGSSGGHGLLDFV
                     NAVKEDAIGVYVTVFPYYGMNRKYTPGIENFETRYFKTFKEKPHAGHSVVCYMGAKVL
                     FDSIEKAGKMDPLAIRQAAMTIDIPLGGTEAGWGVKFDPETGQNTRSPAVLFMWQKDL
                     TLGAVYPPGAAITEGVFRK"
     gene            3797..4684
                     /locus_tag="VEL68_20045"
     CDS             3797..4684
                     /locus_tag="VEL68_20045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38329.1"
                     /translation="MLDKFLAILASGVLLGGIYALIALGLTVILGVVRIMNLAQGAAV
                     MLAMYLAFWIFKVFEIHPYLSAPLIILISFFLGVFVQRFIVQPIPGAIMDMVLIATLG
                     FSIFLENLALFLWGADYRTVHTAINNMILRIGPINVSYPRLVAFIASILSALVLYIFM
                     KRTMIGKALRAVAQDREAAELMGIDSKRMYIYALGVGTALAGLAGVILIPMYYAFPGV
                     GGYFMLAAVVVIFLGGLGNMIGCILGGFIIGILEVFSGYFLTTALKETIYFSAFILIL
                     LIKPTGILGLGKGSERIGM"
     gene            4696..5670
                     /locus_tag="VEL68_20050"
     CDS             4696..5670
                     /locus_tag="VEL68_20050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38330.1"
                     /translation="MSGKTGWAIAAALLLGGIPLFISNPYFLHVLIMIFLYGAISGAW
                     NIIGGYAGQFSLGHAAFFGIGAYTSTLLFLKFGVTPWLGMVAGGVLSALVSVLIFYPC
                     FKLKGIFFALATLAFGEVIMQVSIYWRGLTRGSLGLMIPFRPSFYNLMFEGKLGYYYI
                     TLIFLLGIIFISYSIEQSKIGFYLKAIKDDEDAAEAMAIDTAKCKLMALLISSFFTSV
                     GGSIYAQYITLIEPEVAFSLFGISIQIAIMSIIGGIGTFLGPAFGAFVLVPLGEFIRD
                     LLGGKTPGIHAMIYGIMVILIVILLPKGVMRWISDLVQKKPKGAPAKG"
     gene            5667..6392
                     /locus_tag="VEL68_20055"
     CDS             5667..6392
                     /locus_tag="VEL68_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38331.1"
                     /translation="MILEVENLNKNFGGLRAVRDISFHVSPGEIVGLIGPNGAGKTTL
                     FNLLSGFIEPTSGRILFNQEDISRLRPPHKIIRRRMGRTFQIPKPIRSATVLQNVMIG
                     AFARTHDKQEAQDQAARILEFLKLADLRDTLAESLSLPYLKRLEIARALATQPIFLML
                     DEVMAGLNETEIQEMIATLRECNQRGVTLFIVEHIMAAIMQISQRIIVINFGEKIAEG
                     TPQEIMNNEKVIESYLGEDFSLA"
     gene            6385..>6680
                     /locus_tag="VEL68_20060"
     CDS             6385..>6680
                     /locus_tag="VEL68_20060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ38332.1"
                     /translation="MLKVQTLEVFYGAIQCIRGASLGVEKGEFVSLVGSNGAGKTTLL
                     KTIAGLVKNSSGAVFFQEERIDRLSGHEIAQRGLILIPEGRRLFPYMTVLENLEL"
BASE COUNT         1566 a         1598 c         1847 g         1669 t
ORIGIN      
        1 gaatcgattt tctcaaaaaa gggcctccaa aaaggtcttg acaaggagaa tgggaagcga
       61 tagagtctgg tcctacattt tgtggaataa atgaaatttt ttctacattc caaatgaatc
      121 tctttttcca cgaacaagtc caaagaaaac attccaagct gacccgaagc cagatcgccc
      181 tgacgcgata tattttacac cacgaccgga agcgcctttc ctttgttaaa tttttagaaa
      241 aaccggccaa acgattcttg ctcttccttc agaactgtga ccctattttg agcagcagat
      301 tccttccctt tcttttttcg gctcaggatt ttttcagaat caaaaatcag gcccggaaag
      361 tttcgtaggt taagcgatgt ctaccagcaa actacggtat ctgtttgatc cgctcagaat
      421 cggagccatg catgtcaaga atcggttggt catggcgccc atgggcacca acttggcgag
      481 cctggaaggg aaagttaccc cctccctagt cagctactat caggcccgag cccgaggcgg
      541 cgtaggtttg atcatcaccg acgataccac gatcacctcc tccgcgatct atcatccaca
      601 cggcctgcgt cttgaccagg atcccctcat tcagagctgg aaggaattga cccaggcggt
      661 tcacgtctat ggggccaaga tcgcgcccca actcgttcat cctagcttca atgccccttc
      721 cgtcttttcg ggcgtgcagc cggtcgctgc ttcccccatt ccttcccgcc ggttcaggga
      781 aattccccgg gagctgacgg tcgaggaaat caaggaaatc gtccggcaat tcggggaggc
      841 ttcccgccgg gcccaagaag cggaatgcga tgcggtgcaa attcattgcg cgcatatgca
      901 ccatcttttg ggcggctttc tttcccccct ttataataaa cggacggaca cctatggagg
      961 ttccctggag ggaaggttga gactcccctt ggaagtgatt cagcatatcc ggtccaaagt
     1021 gggccccggg tttcccatac tggtccgcat ttccggggac gaattcctgc ccggaggcca
     1081 gagcctggag gaaagcaaac agatcgcctc gctcctggtg gagggggggg tggatgccat
     1141 tcaagtttcc gcggggacag ggaacaattt ttgggtggta gtccccccga ccggatcacc
     1201 cgaagccccc aacgcgttct tagccgaaca aatcaaaaaa gaagtgaatg tccccatcat
     1261 ttgcgtaggc cggattcacc atccctgggc agctgagaac cttttgaaaa gtggaaaggc
     1321 cgacatggtg gccatgggga gggctctggt ggcggacccg gaacttccca acaaggccgc
     1381 ccggggggat tgggacgaaa tcgcgccgtg tgtgggagat tccatgtgtc tgacctacgt
     1441 gcagatggag gaaagaatcc gctgcttagt caacccgatg gcgggacgcg aggaggaaga
     1501 tccaattccg cccgccgcca ttgccaaaaa cgtgctggtc atcgggggcg gccccgctgg
     1561 aatggaagcc gcgcgagtgg cagccatgag gggccatcgc gtcaccctga tagaaagggc
     1621 ctctaaactg ggaggacagc tgctagtggc atcctttcct cccatgaaac aggaatacac
     1681 ttgcctcatc cagtatctgt cccatcaggt aaaaaaagcc ggagtgaagg tggagttgaa
     1741 cagggaagcc gaccttgccc tcgtgagttc cctccggccg gaaacgatta tcgtagccac
     1801 aggcgcggtt cctttcatcc cgagggatat ccctggattc aaccgagaat gcgtcctcac
     1861 cgcgtgggag gtgctagcgg gtcgaagcct ggtgggtcca aaagtggtcg tcgtgggtgg
     1921 gggaaaagtg gggtgtgaaa ctgccgattt catcgcccat ccggccgatg acctgacccc
     1981 caaaggcaat caggtaacca tcatcgagct gcaggaaaac atcgccctcg acgaaaagag
     2041 catggcgagg agtctgttga tccggcgttt gaaggaaaag ggggtcggga ttattctggg
     2101 cggaaaggtg atcgaaattt tgccggatgg tgtgaaatac gtcaaagagg gccgggagga
     2161 agccttacgg gggatgaacc atgtcgtcct agctatcgga gccgtctcgg aaaatacatg
     2221 ggcccaacgt atcaccggga ttcccacttt tgtgatcggg gatgccaagc atccaaggaa
     2281 tgcccttgaa tccattgccg aaggttggga gattggtcga aaaatataga atgcccaaaa
     2341 agattgtcat cctgggaacc ctggacacta gaagttaata tctggctatg cgctggaaag
     2401 acgcaaatcc ctggccagat gaattggatg aaaggaggaa agaaaagatg aaagggagag
     2461 ggaaagtttt tgtatgggga atggttctga cattagtctt cacccttttg gcgacccaat
     2521 cgatcgtttt tgggtttact gacccggtgc gcatcgggac catatttccc cggagtggct
     2581 tcttggcaga cctcggaatc gaaagctgga gaggggctga gatcgccagg ctggaaagga
     2641 atgaaaaagg gggcatcaag ggtcaaaaag tcatttatgt ggataccgat gctcccaatg
     2701 tggatgccgc agtttccaat gccgagagtt taatcaatcg ggagaaggtg aaagttattt
     2761 gtggaaccta ctggagttcc ggaggatatg ccgccagcgc ggtggcggag aaaaacaaaa
     2821 ccttatattt cgaattcacg tcagtttccg atgagctcac ccagcgggga tttaagtatt
     2881 tgctccgtac cgcaatgctt acctccggga tgggaggggc gattgtcgac tttatcgtcg
     2941 atagcgccaa atccggaaag atcggcgtga agctgcagga cctcaaggtt gcggtcatcc
     3001 acgaagattc gctcatgggc acctccatat ctcagttcat tatggatgac tgtaaaaaga
     3061 aaggcctcaa tgtggtccat tacgaagcct atagcgccaa ggcgaccgat ttatccccct
     3121 tggttttgaa gctgaaggat aaacagaccg atatcttgat ttcggcttgt tatctgacgg
     3181 acgcaatatt attatcccgg cagatgaaag aattagggtt aaaagtgaaa gcgcagtttg
     3241 gttcgagcgg cgggcatgga ttactggatt tcgttaacgc agtcaaagaa gatgctatcg
     3301 gggtgtatgt gacggtcttc ccctattacg ggatgaaccg gaagtatact ccgggaattg
     3361 aaaactttga aacccgctat ttcaagactt ttaaagaaaa accccacgcg gggcattcgg
     3421 tcgtttgcta catgggggcc aaagtattgt tcgattccat tgaaaaggcc gggaaaatgg
     3481 accccttagc cattcgccag gcggccatga cgatcgacat cccgcttggg ggtacagaag
     3541 ccggttgggg cgtcaaattt gacccggaaa ccggccagaa tacgagatcc cccgccgtat
     3601 tgtttatgtg gcagaaagat ttgaccctgg gcgccgttta ccctccaggg gcggccatca
     3661 ccgaaggggt ttttagaaag taaacgaaac cccggttctt ttcggagggc gggcagagga
     3721 ttttctggag aatctccgct cgcccagctt caacctaaaa attttccatc cccaatccca
     3781 aggagatcga accgagatgt tggataaatt cctggcaatt ctggcctcag gggtattgct
     3841 gggaggaatc tacgcgctga tcgctctggg cctgaccgtt atcctggggg tcgttcgcat
     3901 catgaacttg gcccagggcg ctgccgtaat gctcgctatg tacctggcct tttggatctt
     3961 taaggtcttt gagattcatc cttatctctc ggcgcccctg atcattctca tctccttctt
     4021 tctgggcgtt ttcgttcaaa gatttattgt ccagcccatc ccgggggcca tcatggatat
     4081 ggttttgatc gccacgctgg ggttctccat cttcttggaa aatttagctc tttttctctg
     4141 gggggcagat tatagaacgg ttcatacggc gattaacaac atgattctca gaatcgggcc
     4201 gattaatgtt tcctatccaa gattggtagc ctttatcgct tctattttga gcgcgctggt
     4261 tttgtatatt tttatgaaaa ggacgatgat cggaaaggcc ctacgggccg tcgcccagga
     4321 ccgagaggca gcggaattga tgggcataga ctctaagcgt atgtatatct atgccctggg
     4381 ggttggcacg gcgctggcag ggcttgcggg ggttatattg atccccatgt attacgcctt
     4441 tcccggtgtg gggggctatt ttatgttggc tgccgttgta gtgatcttct tgggcgggtt
     4501 gggcaatatg atcgggtgta tattgggcgg gtttattatt ggcattctcg aagtctttag
     4561 tggttatttt ttgacgactg ccttgaagga aacaatctat ttctccgctt ttatcctgat
     4621 tctgctgatt aagcctacgg ggattctggg gttgggaaag ggttcagaac gtatcggaat
     4681 gtgagaaggg gaaacatgtc cgggaaaact ggctgggcca ttgccgcggc tctcttgttg
     4741 gggggaattc ccctttttat ttccaacccc tactttttgc atgtccttat tatgatcttt
     4801 ctctacgggg cgatcagcgg agcctggaat attattggag ggtatgccgg gcagttctcg
     4861 ctgggacacg cggcgttttt cgggattggc gcatatacct ccacgcttct gttcctaaaa
     4921 tttggggtga ctccctggct ggggatggtg gccggggggg ttctctccgc ccttgtttcg
     4981 gttcttatct tctatccgtg ttttaaatta aaggggattt tctttgctct cgccacctta
     5041 gcctttggcg aggtcatcat gcaggtttcc atctattgga gagggctcac gagagggtcc
     5101 ctgggactca tgattccctt tcgcccttct ttttacaatc tgatgttcga aggcaagctg
     5161 ggctattact atattaccct gatctttctg ctgggaatca ttttcatctc ttattctatc
     5221 gaacaatcca agatcggatt ttatttgaaa gcaatcaaag atgatgaaga tgcggccgag
     5281 gccatggcga tcgatacggc caaatgtaag ctcatggcgc tcctcatcag tagctttttc
     5341 acgagcgtgg ggggaagcat ctatgcccaa tacataaccc tgatcgagcc cgaggtggcg
     5401 ttctctcttt ttgggatttc cattcaaatc gccataatgt cgatcatcgg aggaatcggg
     5461 acttttttgg gcccagcttt tggcgccttt gtattggtcc cgctggggga attcatccgg
     5521 gacctattgg gggggaaaac gcccgggatc catgccatga tttatggaat catggtgatc
     5581 ttgatcgtga tcctgcttcc caaaggggtt atgcgctgga tttcagattt ggttcaaaaa
     5641 aaacctaaag gagctccagc aaagggatga ttctggaagt cgaaaacctc aacaagaatt
     5701 ttggggggct tcgggcagtc agggatatca gttttcatgt ctccccggga gagatcgtgg
     5761 gcctcatcgg ccccaatggg gcgggaaaga cgaccctttt taatctcctt tcaggtttta
     5821 ttgagcccac ttcggggcgg atcctcttca accaggaaga tatcagtcgc ttgcggcccc
     5881 cccacaaaat tatccgcaga aggatgggcc ggactttcca gatccccaaa ccgatccggt
     5941 cggcaaccgt tctacagaat gtgatgatcg gcgcttttgc ccgcacccat gacaaacagg
     6001 aagcccaaga ccaggctgct agaatcttgg agtttctaaa attggccgac ctgcgagaca
     6061 ccttggccga aagcttgtct ttgccgtatt tgaaaaggct ggagatcgcc cgggccttgg
     6121 ctacccagcc gatttttttg atgctggatg aggtgatggc cggcttgaat gaaaccgaaa
     6181 tccaggagat gatcgcgact ctaagggagt gcaatcagag aggggtgacc ttattcattg
     6241 tggaacatat catggccgcc attatgcaaa tttctcaaag aatcatcgtt atcaattttg
     6301 gggagaagat cgccgaaggg accccccagg aaatcatgaa caacgaaaag gtcatagaat
     6361 cctatctggg ggaggatttt tcccttgctt aaagttcaaa cgttggaagt tttttacgga
     6421 gccatccaat gcattcgggg ggcttcttta ggggtggaga agggagaatt tgtctccctc
     6481 gtgggatcga atggggccgg gaaaacgact ctgctgaaga ccatcgctgg cctggtgaaa
     6541 aattcctcgg gggccgtctt tttccaggag gaaaggatcg atcggctcag cggacatgaa
     6601 atcgctcaac gagggttgat tttgatcccg gaggggagga ggcttttccc ttacatgacg
     6661 gttttagaga atctggaact
//
LOCUS       DASPRR010000543         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1748539, whole genome shotgun sequence.
ACCESSION   DASPRR010000543 DASPRR010000000
VERSION     DASPRR010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748539"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VEL68_20065"
     CDS             <1..211
                     /locus_tag="VEL68_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38333.1"
                     /translation="KDFIGDFREQGIRDIGLMARKQAVSGACVEFMVLGGLAQGQPFT
                     EMDVIIDLAKNRQKEIGRFTCTGEV"
     gene            216..704
                     /gene="mlaD"
                     /locus_tag="VEL68_20070"
     CDS             216..704
                     /gene="mlaD"
                     /locus_tag="VEL68_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HXZ38334.1"
                     /translation="MKKYAMETAAGVFVVIGLACIAYMTVKLGHVSFLGEATYPLFAK
                     FTSVSGLRPGSPVDMLGIEVGRVERITLDQKAQLAVVEMKIREGIQVYGDAIASIKTE
                     GLIGDKYLRIDPGGAESPLKPGGIITETQPAVDVADLIGKYAFGEVKKPEGGDTAPRS
                     KP"
     gene            701..1294
                     /locus_tag="VEL68_20075"
     CDS             701..1294
                     /locus_tag="VEL68_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38335.1"
                     /translation="MTRLLKVAIILVLFLLALSVFAGEPLDAVKANVNKVLDVLRDPN
                     LKTDSAKEIKKEKLRAIYSQMFDEVELARRTLARNWGKLNPSQQEEFIRLFRQVLEKA
                     YIDKILAYTNEKIVFSREVELSSDQAEVQTKIVTSSREIPIFYRVIRKDSVWKVYDVI
                     VENVSLVQNYRSQFNSILANKTPDQLLEILRKKVQEA"
     gene            1291..>2155
                     /locus_tag="VEL68_20080"
     CDS             1291..>2155
                     /locus_tag="VEL68_20080"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HXZ38336.1"
                     /translation="MSRLLFTALFLTFLTVACVHSPSRISSLASPAAPFVSPLSGNAS
                     GSEQDVPTSGGISADSESGNQDSRKSLQEPEQGAKVAAKSQDESPRSQENLRDQEKWG
                     EEEEATIADPLEPFNRAMFHFNDKLYFWALKPVAQGYNWVVPEKARISVKNFFTNLAF
                     PIRFVSCLLRADFKCAGSEFGRFTVNTVWGVGGLLDPSSSTQLKLPKGDADLGETLGV
                     YGLGQGFYFVWPVLGPSSARDSVGLVGGFFLYPVSYIRPWPAWLGVRTYQEVNDASLR
                     IGDYESLKEAAI"
BASE COUNT          488 a          564 c          630 g          473 t
ORIGIN      
        1 gaaggatttt atcggggatt tccgggagca aggaatccgg gacatcgggc ttatggcccg
       61 caagcaagcg gtttctggcg cttgcgtcga gttcatggtc ctgggcggac ttgcccaggg
      121 ccaacccttt acagaaatgg atgtgatcat agatttggcc aagaaccgac aaaaggaaat
      181 cgggcggttc acctgcaccg gggaggttta aaagggtgaa aaagtatgcc atggaaacag
      241 ccgcaggcgt ttttgtcgtc atcgggcttg cctgcattgc ttacatgacc gtaaaattgg
      301 gtcatgtgtc ctttctgggc gaagccacct accctctttt cgccaaattc accagcgtga
      361 gcgggctgcg gcccggcagt cctgtggaca tgctgggaat tgaggtcgga cgggtggaac
      421 ggattaccct ggaccagaaa gcccagttgg cggtggtgga aatgaagatc agagaaggca
      481 tccaggttta tggcgacgcc attgcttcga tcaaaacgga agggctgatt ggtgacaagt
      541 atctccgcat agacccgggg ggcgccgaat cgccccttaa accgggtggg atcatcaccg
      601 aaacccagcc ggcggtggac gttgccgacc tgatcggtaa gtatgccttc ggcgaggtga
      661 agaagccgga ggggggcgat acggccccga ggagtaagcc atgaccagac tgcttaaggt
      721 ggcaattatc ttggtgctct ttcttttggc cctttccgta tttgccggcg agcctctcga
      781 cgcggtcaag gcgaatgtca acaaggtgct cgacgtgctg cgcgatccta acctgaaaac
      841 cgattcggct aaagagatca agaaagagaa actccgagca atttattccc agatgttcga
      901 tgaagtcgaa ctcgccaggc ggaccctggc ccggaattgg gggaagctga atccctccca
      961 gcaggaggag ttcatccgac tctttcgaca ggtattggaa aaggcttaca tcgataagat
     1021 ccttgcctac accaacgaaa aaattgtctt ctcccgagaa gttgagcttt cgagcgatca
     1081 agccgaggtt caaacgaaaa tcgtcacctc gtcgagggaa atccccatct tctaccgggt
     1141 catccggaag gacagcgttt ggaaagtata cgacgttatc gtggagaatg tgagcctggt
     1201 ccaaaactat cgcagccagt tcaattccat cctggccaac aaaactcccg accaactgct
     1261 tgagattctg cggaaaaagg tgcaggaggc atgagccgcc ttttatttac ggcgttgttt
     1321 ttgacctttc ttaccgtggc ctgcgtgcat agcccttccc gtatttcttc cctggcatca
     1381 cccgcagcac cttttgtttc ccctttgtcc gggaatgctt ccgggtccga gcaggacgtt
     1441 ccaacttccg ggggcatctc agccgattcc gaaagcggga accaggatag ccggaagagc
     1501 cttcaggagc cggagcaggg cgcgaaggtg gccgctaaat cccaagacga aagtccccgt
     1561 agccaagaaa atctccggga ccaagagaaa tggggggagg aagaggaggc aacgatagcc
     1621 gacccgttgg agccctttaa ccgggccatg ttccacttca acgacaagct gtacttctgg
     1681 gcgctgaaac ccgtcgccca gggatataac tgggtcgtgc ccgagaaggc ccggataagc
     1741 gtgaaaaatt tcttcaccaa tctcgctttc cccatccgct ttgtcagttg tctcctccgg
     1801 gctgacttca agtgcgcggg ttcggaattc gggcgtttca cggtgaacac cgtctggggc
     1861 gtcggggggc tattggaccc ttcctcaagc acgcagttga aactccccaa aggagatgcg
     1921 gaccttggcg aaaccctggg ggtttatgga ttaggacagg gtttctactt tgtttggccc
     1981 gtactggggc cctccagtgc tcgggattcg gtggggttag tcggcggctt tttcctgtat
     2041 cccgtcagct atattcgtcc ctggcccgct tggctgggag tgaggaccta ccaggaggtg
     2101 aatgatgcct ccttgaggat cggggactac gaatctctca aggaagccgc catcg
//
LOCUS       DASPRR010000544         4261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_135554, whole genome shotgun sequence.
ACCESSION   DASPRR010000544 DASPRR010000000
VERSION     DASPRR010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135554"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VEL68_20085"
     CDS             complement(<1..220)
                     /locus_tag="VEL68_20085"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXZ38337.1"
                     /translation="MKISEYFKTARGRGVIATADGKGKVDVAIYASPHVIDETTVAFL
                     MPDKLTHANLQSNPHAAYLFTEEEEKFKG"
     gene            complement(522..1904)
                     /locus_tag="VEL68_20090"
     CDS             complement(522..1904)
                     /locus_tag="VEL68_20090"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HXZ38338.1"
                     /translation="MDEQIVFGDRMIPVKLPERTKFLPQGLSTSLPPAADLKTAIQEA
                     LRRPLDLRPLRDLAKPGWKVTVAFDDPTVPCYGPVWETAIRSVLEDLEHAGVKKRDVH
                     LLCANALHRKFTRRELAKVIGKGLVEEFGYRLLCHDAEDRENLVHLGTTPNGYDVEVN
                     RLVTDSDLTVYINTVVWRGFNGGWKSICVGLSTYRSIRWHHSPEGMSMSVERNPMHEM
                     LDEMGALLEEKIGKGRIFKMETVLANPLQVARLWTGTVGETRRAALEVLKKQYPPRRE
                     MAEEKADILIYGIPDWSPYASFSVMNPLLTLVSTGLGYLGGVIEALGKPGCSVILATP
                     CPDQWDDAHHPPYREVWREVLGRSRDPYEIRDLFEEDFAHRPEYLYKYQFCYGFHPIH
                     GILATYPLKRLKHAARVLVAGAQNPSLVTHLGFEPTETVEAAIARAEQFHGKDSTIIF
                     IRNPLVRSRQ"
     gene            complement(2076..2807)
                     /gene="gspN"
                     /locus_tag="VEL68_20095"
     CDS             complement(2076..2807)
                     /gene="gspN"
                     /locus_tag="VEL68_20095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HXZ38339.1"
                     /translation="MRIAKYILGYILLGGFFFYIFFFFGQFPYERLKEGLIQSFEESV
                     PLSLSIGRIAPSFPLNLGMENIRVGRDGFSLQLPDLIVHPNLVEILLGNPSFEFSDGK
                     NSSRLTGKFQQGKNQTRLNLRLNVLEIQASIGKGQTLRIKASGEASFQWEGEDLNRAR
                     GQLWSLLERGELQGSAASQIPLPLTMFDTLRAEVQVQDGIARVKRLEASGKETRFSLP
                     QPIQVPLKGGGLPPELFLLFQMPPR"
     gene            complement(2818..3327)
                     /gene="gspM"
                     /locus_tag="VEL68_20100"
     CDS             complement(2818..3327)
                     /gene="gspM"
                     /locus_tag="VEL68_20100"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HXZ38340.1"
                     /translation="MRISGREKVFLRGGAFLLAALLVFLLVVDPALKRAKDLKRLIPQ
                     KEQELKQLRLLHKELESLKETKAALLQKVPAEEKALPPLSRLDGWIERSGLRPSVKSI
                     KPSPSPGGGGMVVDVLMEKAELPLLTRFLYEAQSSPGGFRVARIALKPRYTTPRYLDV
                     SLQLILYQG"
     gene            complement(3324..>4261)
                     /locus_tag="VEL68_20105"
     CDS             complement(3324..>4261)
                     /locus_tag="VEL68_20105"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXZ38341.1"
                     /translation="GALLDLGQEKATLILWQGNRMTLTRSIPISGGGPTAAANPNIVR
                     LAEEVKRTFLSAGFSAGGQSVEKVYLTGGGSLAPGVDQSLSQSLGNPVSALDLGEAAG
                     EAPKEHLPVLGAALGAAWGEPSADWVNLRREEFAPLQKAQRARGRLKILISYAIILGI
                     LGIASLFLNLYLQERKYRDLKAQIRKEFFQALPEVKKVVNELQQMKARVQEEKAKLGS
                     LGGGPGGGSPLDVLRELSLMVDPGAKVRVTELLVDPESIEVNGEADSFDTVNQLKARL
                     DRSGNFKDVQLKTARASSLENVIEFKFQMKQGAGG"
BASE COUNT          885 a         1278 c         1161 g          937 t
ORIGIN      
        1 cccccttgaa tttctcttcc tcttcggtga aaagataggc ggcgtggggg ttggattgaa
       61 ggttggcatg ggtcaacttg tcgggcatga ggaacgcaac cgtagtttcg tcgatcacat
      121 ggggggaagc ataaatagcc acgtccactt tccctttccc atccgcagtg gcaatgacgc
      181 cgcggcccct cgccgttttg aaatattcac tgatcttcat ggttcttcct cctggtacca
      241 attggatttt tccccggccg atgcttttgc ccagaagctc ctccccccgg gccacaaccg
      301 aggcatttca gaaatgtcaa tccccctcag ccggaccccg gcagagccgg gggttcacca
      361 tactgaaatt acgatccatt ctacccaaaa aaactgccct ttgcatctgc ccctttcgat
      421 gtccctcccg gctccaggat ttcaggttcc aatgccaaac ccccgggttt cggaaaggtt
      481 gaatattact tggaacctga accctggccc cgggtcccct gctattgtct gcttcgtacg
      541 agtggattcc ggataaagat aatcgtggaa tcctttccat gaaattgctc tgccctggct
      601 atagccgcct ccacagtctc cgtgggttca aagcccaaat gggtcaccag ggaggggttc
      661 tgcgcaccgg ccaccaacac ccgggctgca tgctttagcc ttttcaaggg ataggtggcc
      721 aggatcccgt ggatcgggtg gaaaccatag caaaattgat atttgtacag gtactcgggc
      781 cggtgggcga agtcttcttc gaagaggtcc cggatctcgt aagggtccct ggaccggcca
      841 aggacctcgc gccagacttc ccggtaggga ggatggtggg catcatccca ttgatccggg
      901 cagggggtgg ccaggatcac cgagcaaccg ggctttccca gggcttcgat cactccgccc
      961 aggtatccaa gaccggtgga gaccaaagtg agaagggggt tcatcactga aaaggaggca
     1021 tagggactcc agtctggaat cccatagatc aggatatccg ctttttcctc ggccatttcc
     1081 ctgcggggcg gatactgctt tttcaggact tccagggcgg ccctccgggt ctcccctacg
     1141 gtgcccgtcc acaaccgggc cacttgaagg ggattggcga gaaccgtttc catcttgaag
     1201 attcgccctt tcccgatttt ctcctccagg agcgctccca tttcatccag catttcatgc
     1261 atcgggttcc gctcgacgga catggacatg ccctcaggag agtggtgcca gcggatggat
     1321 cggtacgtgg aaaggcccac gcagatcgat ttccacccgc cgttgaaccc tctccacacc
     1381 acggtattga tataaacggt caggtcacta tcggtaacga gccggttcac ttccacatcg
     1441 tacccgttgg gtgtagtccc caggtggacc aggttttccc ggtcctcggc gtcatgacag
     1501 agcagccggt atccaaactc ctcgaccaag cctttgccga tcaccttggc caactccctg
     1561 cgggtgaatt tccggtgaag ggcattggcg cacagaagat ggacatccct tttcttcacc
     1621 ccggcgtgtt ccaggtcttc cagaaccgaa cgaatggccg tttcccatac aggcccatag
     1681 cagggaaccg tgggatcgtc aaaagccacc gtgactttcc agcccggttt agccaggtcg
     1741 cgaaggggcc gaaggtcgag gggccttctc aaagcctcct ggatggcggt cttgaggtca
     1801 gccgcaggcg gaagagaagt ggaaaggccc tggggcagga acttcgtccg ctctggaagc
     1861 ttgaccggaa tcatccggtc gccgaacacg atctgttcat ccataatggt ccccaaaaac
     1921 cacgaatttt ctctcgctcg cttcgctaga gacgcagagc tcacagagga gattaatata
     1981 ttttcaaaat acaacccaaa attcaacagg ttattttttc gtatttttct ctgcgatccc
     2041 tgtgggctct gcgagagaca aacgtctttc cgtccttacc tcggaggcat ttggaagaga
     2101 aggaacagtt cggggggcag gcccccgccc ttcaggggga cctggatggg ctgggggagg
     2161 gaaaatcggg tttctttgcc agaggcctcc aggcgcttca cccgggctat cccgtcttgg
     2221 acttggacct ccgcccgcag ggtatcaaac atggtcaagg gtaggggaat ttgagaggcc
     2281 gcgcttccct gaagctcacc cctctccagc agactccaga gctgccccct cgcccggttt
     2341 aagtcctccc cttcccattg gaaagaggct tctccgctcg ctttaatccg cagggtttgc
     2401 ccttttccga tggaagcttg aatctccagc acgttgagcc gcaggttcag tcgagtctga
     2461 ttctttccct gctggaactt tcccgtgagg cgggaggaat ttttcccgtc gctgaattcg
     2521 aaactgggat ttcccaggag gatttccacc aggttgggat gaacaatcaa atctgggagc
     2581 tgaagcgaga aaccatccct tcccacccgg atgttctcca tccccagatt caacgggaag
     2641 gaaggagcga ttcgcccgat ggaaagggac aggggaacgg actcctcgaa actctgaatc
     2701 aacccttcct tcaatcgttc ataaggaaac tgcccgaaga aaaaaaagat gtagaaaaaa
     2761 aaacctccca ggaggatata tcccaaaata tacttggcga ttctcatggc cgctctctca
     2821 tccctggtag aggatcagct gcagggagac gtcaaggtat cggggcgtgg tgtaccgggg
     2881 cttaagggca attctggcca cccggaaacc gccgggagag gattgggcct cgtagaggaa
     2941 gcgggtgagg agggggagtt cggctttttc catcagcaca tccaccacca tcccccctcc
     3001 ccccggggaa ggggagggct tgatggactt cacgctcggg cgcaggcccg accgttcgat
     3061 ccacccgtcc agccgggaaa ggggcggcag ggccttctct tccgcgggaa ccttctggag
     3121 aagggccgcc ttggtctctt tcagcgactc gagctccttg tgcagaaggc ggagttgctt
     3181 gagttcctgc tccttctggg ggatgaggcg cttcaggtcc ttggccctct tcaacgccgg
     3241 gtccacgacc agcagaaaga ccagcagggc agccagaaga aaagcccctc cccggaggaa
     3301 gaccttctcc cttcccgaga ttctcatccc ccggccccct gcttcatctg aaacttgaac
     3361 tcaatcacgt tttccaggct gctggcccgg gctgttttca actggacgtc cttgaaattg
     3421 cccgaccggt ccagacgggc ctttaactgg ttcacggtgt caaaggagtc cgcctctcca
     3481 ttcacttcga tcgactccgg gtcgacgagt aattccgtca cccttacctt ggcccccggg
     3541 tctaccatca ggcttaactc ccgaaggaca tccagcgggg acccgccacc cggtcctcct
     3601 ccaagagagc ccagcttggc tttctcttct tgaacccgcg ccttcatctg ctggagttca
     3661 ttgaccacct tcttcacctc gggcagagcc tggaagaact ctttccggat ttgggctttc
     3721 aggtcccgat atttcctttc ttgaaggtaa aggttcagaa aaagggatgc aatccccagg
     3781 attcccagaa tgatggcata ggaaatcaga atcttgagcc ggcccctggc tctctgagct
     3841 ttctgcaaag gagcgaattc ctcgcgccga agattgaccc aatcggctga aggctcaccc
     3901 cacgcggcac ccagggccgc acccagaacg gggaggtgct ccttgggggc ttcacccgct
     3961 gcctccccca ggtccaatgc ggaaacgggg tttcccaggg actggctcaa gctctggtca
     4021 acgccgggag ccaatgaacc gccccccgtg agatagactt tctctacgga ttggcctcct
     4081 gcactgaatc ccgcggagag gaaggtccgt ttaacctctt cagccaggcg gactatgttg
     4141 ggattggccg cggcggttgg tccccccccg gagatgggaa tagacctggt cagggtcatt
     4201 cggttccctt gccataggat cagcgtggcc ttttcctgcc ccaggtccag gagggccccc
     4261 g
//
LOCUS       DASPRR010000545        13576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2069661, whole genome shotgun sequence.
ACCESSION   DASPRR010000545 DASPRR010000000
VERSION     DASPRR010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13576)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13576
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069661"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..862
                     /gene="rpsB"
                     /locus_tag="VEL68_20110"
     CDS             56..862
                     /gene="rpsB"
                     /locus_tag="VEL68_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXZ38342.1"
                     /translation="MSTVTMKQLLEAGVHFGHQTKRWNPKMKEYIFGSRNGIYIIDLQ
                     KTVRLFKEAYKFIVDTCSKGGTILFIGTKKQAQDSILEEATRAGMFFVNQRWLGGTLT
                     NFRTIKKNIDRMKKLEKMKEDGTAEALPKKEVLQMDKLHAKMEKTLGGIKDMTKLPDG
                     IFIVDPRKERIAVHEARKLGIPIVAIVDTNCDPDEIDLIIPGNDDAIRAIRLISSKVA
                     DACLEGKQLREAQLSSEGAAAPPVEGIPPQPGGAGSDGDQKEPEGEEDQE"
     gene            877..1485
                     /gene="tsf"
                     /locus_tag="VEL68_20115"
     CDS             877..1485
                     /gene="tsf"
                     /locus_tag="VEL68_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXZ38343.1"
                     /translation="MEVSANMVKELREKTGAGMMDCKKALAETGGDFQKALDYLRQKG
                     LATAAKRAGRVASEGRIGSYIHAGGKIGVMVEVNCETDFVAKTDDFQAFAKDIAMHIA
                     ASSPSYIRREEVTVEVLAREKEIYRAQARDAKKPEKVIDKIVEGKLEKFYGETCLLEQ
                     PFVKDPDLTIQDLLNGLIGKLGEKIEIRRFTRYQVGEGIAKS"
     gene            1482..2243
                     /gene="pyrH"
                     /locus_tag="VEL68_20120"
     CDS             1482..2243
                     /gene="pyrH"
                     /locus_tag="VEL68_20120"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXZ38344.1"
                     /translation="MKTSAKPVYRRILLKLSGEAMMGDQEYGISPAVVQAVAQEVKEI
                     RGLGVEVGIVIGGGNLYRGVAASAEGMDRTSADHMGMLATVINSLALQDALEKIGIFT
                     RVLSAIEMNRIAEPYIRRRAVRHLEKGRVVIFAGGTGNPFFTTDTAAALRAMEVKAQV
                     ILKATKVDGVYDRDPLIHKKARKFEELSYIQVLNLGLRIMDATAISLCMENRLPIIVF
                     NLTQKGNIKRVVLGRKIGTRIGGGEPHVKKGSVPQ"
     gene            2215..2778
                     /gene="frr"
                     /locus_tag="VEL68_20125"
     CDS             2215..2778
                     /gene="frr"
                     /locus_tag="VEL68_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXZ38345.1"
                     /translation="MLKKEAFLSDLKSRSEKSLEALRKEMGRVRTGRASQAILDGVRV
                     NYYGAPTPLAQVASLSVPDARTIMIQPWDSKVLGEIEKAIQKSDLGLTPLNDGKVVRI
                     NIPPLTEERRKELVKVIKRMEEECKVALRNLRREANEQLKTAKKDKQISEDDQFKSQD
                     EVQKLIDKLIQKAEEIVKAKEKEVLEI"
     gene            3011..3754
                     /locus_tag="VEL68_20130"
     CDS             3011..3754
                     /locus_tag="VEL68_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXZ38346.1"
                     /translation="MHRLLKEKLPHHLAIIMDGNGRWAEKRSLDRVEGHRQGAESVRT
                     IVRASREIGLPYLTLFAFSSENWSRPRAEVDALMLLLKNYLSSELQEMLDRQIRLLAV
                     GELSRLPKEVQTTLYDTMKKTGRATGMTLTLALSYGGRDDILQAIRRLMFHCQDRSLT
                     PQEITEEVFSKFLWTANLPDPDLLIRTSGEMRISNFFLWQLAYAEIYFTPTLWPDFSK
                     EEFIQALLSYQNRERRFGLTSEQIRAEGG"
     gene            3759..4562
                     /locus_tag="VEL68_20135"
     CDS             3759..4562
                     /locus_tag="VEL68_20135"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXZ38347.1"
                     /translation="MHLQRWISGLILGPSLILLIVFSPPWLFLLFILLVIFLALREFY
                     ALCLPEVSPGEKGAGFILGFLVAVSLYSREPRGFLFVILLAFFFLSIRALFRPEAFPA
                     RIETVSKHLLGLFYLPFLLSHFVLMRNLERGRLWVLFTLVAVYFGDTLAFYVGRAWGK
                     KKMAPQISPGKTVEGGLGAVAGSVAGALVSKLIFFPSIPLLHALILGASVGVIGQLGD
                     LWESLLKRSVQVKDSGALIPGHGGLLDRIDSVLFAAPLVYYYAWRMGAG"
     gene            4559..5719
                     /locus_tag="VEL68_20140"
     CDS             4559..5719
                     /locus_tag="VEL68_20140"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXZ38348.1"
                     /translation="MKTIAILGSTGSIGVNSLEIIAKFPEEFRVVALAGGKNYRKMEE
                     QVRLFRPDVASVMDPEVARRLKQSLSSIPTKILWGNEGLNAVATHPQSQMVVSALVGS
                     VGLLPTLAAIRAGKSIALANKEALVMAGEVIMKEIRETRGNLLPIDSEHSAIFQALAG
                     HRKEEIKRIILTASGGPFLDTPPEELKRVTPREALNHPQWRMGKKVTVDSASLMNKGL
                     EVIEAHWLFGVPASRIEVHIHPQSIVHSMVEYIDGSVIAQMALPDMRGPIAYALSYPQ
                     RLDLRLPSLNLFEAGPLSFKPVDRERFPALALAYQALETGGTMPAVLNAANEVAVEAF
                     LEGRLEFLKIPSVIRKTMKNHQPLRLNSVEDVLEAHSWAREEARETLNRGNL"
     gene            5716..6783
                     /gene="rseP"
                     /locus_tag="VEL68_20145"
     CDS             5716..6783
                     /gene="rseP"
                     /locus_tag="VEL68_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXZ38349.1"
                     /translation="MTITIIATIIVLGVLIFVHELGHFLAAKGFRVGVLTFSLGFGPK
                     LFGKKVGETQYQVSMIPLGGYVKMVGEEPGEKIPPEMIAKSFSHQRVSRRLGIVFAGP
                     FFNFLFAVVAFAAVFVVGMPTLLPEVGEVKPDLPAYQAGLQKGDRILEVNGSPAKRWE
                     DLAKVIHESADRPLVLKVEREQKTFQVTVIPQGSLQKNIFGDDVQVGLIGISPSGNTF
                     IERVDPFSALYRAVLQSWRVTELTVISIYKIIEGKISAKTIGGPILIAQLAGQQAKAG
                     ALSLVIFMAVISINLVILNILPIPVLDGWHLLIFLIESVVGRPVSLKLRERAQQVGIF
                     IIILIMLLVFYNDITRIIGPE"
     gene            6788..7534
                     /gene="tsaB"
                     /locus_tag="VEL68_20150"
     CDS             6788..7534
                     /gene="tsaB"
                     /locus_tag="VEL68_20150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXZ38350.1"
                     /translation="MLILAVETSTRTGSIALVEALGEDPGAPLLKIIGETTLNLAETH
                     SSRLMPAIDRLLRETSQPIQTIQAIAVGLGPGSFTGLRIAISTVKGLAFALRVPVMGA
                     PTLDVLAQNLSFASIQVCPVLDARKKEVYAALFQGKGEGQLTKMSEDWVLSPEDLCSR
                     ITERTILVGNGGEVYGDIFRDRLGPRVLFAHPEDSFPRASNVVRLSLPAWRKGKTLDL
                     FGFTPIYVRRSEAEIHWDQKARGNPLSEGR"
     gene            complement(7630..8754)
                     /locus_tag="VEL68_20155"
     CDS             complement(7630..8754)
                     /locus_tag="VEL68_20155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ38351.1"
                     /translation="MADMLKGKPKVLDSIPASFILTDTHTRILYVNQQAEVFFGYTQE
                     ELEGQHLRILFLEDDLLYFLPNIVFLTRYKNGFEGEVLLKQKDGTRIFVHLSTSCFQE
                     GGEAFLTFYFQEIQRLKVLEKERLEMERWAGLGRMVAEIAHQFRNPIASIGGYTRRLS
                     KNWPRSSKNLPYLKQILKQTGRLENILQRVEEYVQIPRPAFRREKIQKLVEDVLPRIS
                     HRAQEKEASFRLDTRGMEGNGRLFIDADLIGKALTHLLENSLQAIPPKSKGGGKKVVD
                     LALFDDGDNVGVSIADRGVGIPKKNLSLIFEPFFSTHPGRVGLGLTFVRRVMEEHRGR
                     IRVDSRLKKGTTVTLYFPKDRRRKVRREFVSPEAMEKEEG"
     gene            8912..9628
                     /locus_tag="VEL68_20160"
     CDS             8912..9628
                     /locus_tag="VEL68_20160"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXZ38352.1"
                     /translation="MLDYLLVSRGGSSRCGVEKGEYPEYNRHAMMEDRPKIRCVIYDC
                     DGVLIDSLNANRWFYSFLCAATGRFPLAEEELQYAHTHTVHEAIHHIFRDDPGLEEKA
                     LGVFSRMDPEESMSRLKLEPHLIPTLEALKAKGILRAISTSRTTNVEVLLRRFSLERL
                     FDLVVTSRDVLNPKPHPESIEKVLRALVIRNDEALFVGDSETDGRAARSAGVKFIAYR
                     NSGIAADGFIEDHPDLLKFL"
     gene            complement(9984..10343)
                     /locus_tag="VEL68_20165"
     CDS             complement(9984..10343)
                     /locus_tag="VEL68_20165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXZ38353.1"
                     /translation="MIKVKEYNHIGIVVRNLEKCKWFYGTFLGLQTIARPPFNFPGHW
                     YQVGPNCQLHLMGYEEDIPDTMRHIALEVANFEETVRELRAKGIEIIDGPAKRMDGSD
                     YLFCKDPDGNRVEITHH"
     gene            complement(10645..11538)
                     /locus_tag="VEL68_20170"
     CDS             complement(10645..11538)
                     /locus_tag="VEL68_20170"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ38354.1"
                     /translation="MIIESKGKITEEFYALGNSALPSYLLMGGSPVLFDSGMTVMGPL
                     YLQDLKKTLGDPNRLSYLFLTHSHFDHAGSAPFLKRNIPGLKVAAGKLAADIFKRPNA
                     IQLIQSLSRPMEEKFKSQVGEEDVAFRGLDLDRVLEDGEEIELERGTAIRVIATPGHT
                     RDAVCYYIPKLKALVGGEAVGTFDRQFNVRPVFLSSYDDYLSSLEKLRTLKIDLLLLG
                     HHFALTEDAQAMIPKAIEATQAYGKRIEEELKANGGNQDAVVKKVFQEDYVERKLINQ
                     EERPFLINLTAQVKAMGERKK"
     gene            complement(11585..12217)
                     /locus_tag="VEL68_20175"
     CDS             complement(11585..12217)
                     /locus_tag="VEL68_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38355.1"
                     /translation="MTNTLHRKGTPSSLKNDYVIFCTMAKGINSQGSGPKIQEFIRIC
                     LKYNPVNMGDGYLGNILQEDVGIQKFLSERNDGEGAAAVFTDLDTLRKVIDELIQADL
                     RISINISGLLDEVRECCRRAGIERHSVEHSLGVWGAKDRLPEREILEVNTLCGHGMVS
                     FNLIRKMVEYVKLRRLTSQKAANLMAKCCECGAFNPVRAEQLLEKMVKRE"
     gene            complement(12221..13030)
                     /locus_tag="VEL68_20180"
     CDS             complement(12221..13030)
                     /locus_tag="VEL68_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ38356.1"
                     /translation="MEIDKKKCVGCGNCHTICTMGAIYLAEDGKSEVNQEECVECNTC
                     YRVLRNEGYSPWFVRVIRKVLSTFRLGYLAEVDVCPTGALTPPELEWPRNLRAAFSDP
                     TTVHPGTGLGGRGTEEIKSNDVTGRIRKGEAGIVVELGRPGIGAHFRDVEKIAMTMAR
                     LEPFFEPQNPVTQLIVDPRAGKMKEEVLDEKVMSAIIEMKVKLEKIPEILHALERGGR
                     EVDTVISVGVSSKCLPDGSAPHEEWVRKAGYSLSPNGKTNLGLGRPFFKED"
BASE COUNT         2993 a         3812 c         3675 g         3096 t
ORIGIN      
        1 tcggatcaac ggccgggaaa agggaagtgg aggaaaaacc caggaggaaa aatctatgtc
       61 caccgtcacc atgaagcagt tgctggaagc cggagttcat tttggccacc aaaccaagag
      121 gtggaatccc aagatgaagg aatacatttt cgggtcccga aacggaattt acatcatcga
      181 tcttcaaaag accgtccggc tctttaagga ggcctacaag ttcatagtcg acacttgcag
      241 caaggggggg acgatcctct tcataggaac gaagaagcag gcccaggatt ccatcctgga
      301 agaggccacg cgcgccggaa tgttcttcgt caaccagaga tggctggggg ggacgctgac
      361 taatttccgg accatcaaga agaatatcga ccgcatgaag aaactggaaa aaatgaagga
      421 ggacgggacc gcggaggccc ttcccaagaa ggaagtcctc cagatggaca agctgcacgc
      481 caagatggaa aagaccctgg gcgggatcaa ggacatgacc aagctgcccg acggaatctt
      541 catcgtcgac ccccgcaagg agaggattgc ggttcatgag gcccggaaac tgggaatccc
      601 catcgtggcc atcgtggata ccaattgcga tcctgacgag atcgacctga tcatccccgg
      661 caacgacgac gccatccggg cgatccgcct gatctcctcc aaggtggccg atgcctgtct
      721 ggaaggcaag cagcttcgcg aggcccagct ctcctccgaa ggagccgccg ccccgccggt
      781 cgaggggatt ccgccgcagc ccggaggggc cgggtcggac ggggaccaaa aagaacccga
      841 gggtgaagaa gaccaagaat aaggaggacg gacttcgtgg aagtatcagc caatatggta
      901 aaagaactcc gggagaaaac cggcgcagga atgatggatt gtaaaaaggc cctggccgaa
      961 acgggcgggg actttcagaa agctctggat tatctgcggc agaagggtct ggccacggcg
     1021 gccaagcggg ccggccgggt ggcctcggag ggaaggatcg ggtcctacat tcacgccggc
     1081 gggaagatcg gggtgatggt ggaagtcaac tgcgagacgg acttcgtggc caagacagac
     1141 gatttccagg cctttgccaa agacatcgcc atgcacattg ccgccagcag tccctcctat
     1201 atccgcaggg aggaggtcac cgtcgaggtt ctggcacgcg aaaaagagat ttatcgggcc
     1261 caggcccgag acgccaagaa gcccgaaaag gtgatcgata aaatcgtaga agggaagctg
     1321 gagaagttct acggggaaac ctgtcttctg gagcagccct tcgtgaaaga cccggacctt
     1381 accatccagg acctgctgaa cgggttgatc ggaaaattgg gggaaaagat cgaaatccgc
     1441 cgtttcaccc gctatcaggt aggagagggg atcgccaaga gttgaaaacc tcggccaagc
     1501 cggtttaccg gcggatcctt ctcaagctca gcggcgaagc catgatgggc gaccaggagt
     1561 acggcatcag tccggccgta gtccaggccg tggcccagga agtcaaagag atcaggggcc
     1621 tcggtgtgga agtgggtatt gtcatcggcg gcggcaacct ctaccgcggc gtcgcggcca
     1681 gcgcggaggg aatggaccgg acctccgccg accacatggg aatgctggcc acggtgatca
     1741 acagcctggc cctgcaggat gctttagaaa aaatcggcat cttcacccgg gtcctgtcgg
     1801 ccatcgagat gaatcggatt gccgagccat acatccgccg ccgggccgtt cggcatctgg
     1861 agaagggccg ggtggtgatt tttgccggcg ggacgggtaa ccccttcttc accactgaca
     1921 cagcggccgc tctgcgggcc atggaagtga aggcccaggt aatcctgaag gccaccaagg
     1981 tggacggggt ctatgatcgg gaccccttga tccacaagaa agcaaggaag ttcgaagagc
     2041 tttcttacat ccaagtcttg aatcttgggc tccgcatcat ggatgccacg gcgatttccc
     2101 tgtgcatgga aaatcgtctt cccattatcg tcttcaacct gacccagaaa ggaaacatca
     2161 aacgagtagt gctgggtcgg aagattggaa ccagaatcgg ggggggagag ccgcatgtta
     2221 aaaaaggaag cgttcctcag tgatttaaag tccaggtccg aaaagtccct ggaggccctg
     2281 cggaaggaga tgggccgggt gaggaccggc cgggcctctc aggcgatcct agacggcgtc
     2341 cgagtgaatt actacggagc tccgactccc ttggcccagg tggcttctct ttcggtcccc
     2401 gacgcccgaa cgatcatgat tcagccctgg gattctaaag tcctgggaga gatcgagaag
     2461 gctatccaga aatccgattt gggcctgacc cctctcaatg atggaaaggt tgtgcggatt
     2521 aacattcccc cgctgaccga ggagaggcgc aaagagctgg tcaaagtcat caaacggatg
     2581 gaagaggaat gcaaggtggc cttgcggaac ctccggcggg aggccaacga acaactgaag
     2641 acggccaaaa aggacaagca gatctccgaa gacgaccagt tcaaatccca ggacgaagtt
     2701 cagaagctaa tcgacaagct tatccaaaaa gccgaggaga tcgtcaaggc caaggaaaaa
     2761 gaagtactgg aaatttaaat ctgcattctt gggaggatct tgcaaaattc acttacccgg
     2821 tcattccggc gaaagccgga atccagaatt catttaaaat cctggattcc gggtcgcgct
     2881 ccgcttgccc ggaatgacga tttcctcttg ataggagagt tttgcaagag gctcttgaaa
     2941 attcgacttt ttacgagttc aacaactttc aatcaccaaa acgtcgcgca cccccatata
     3001 atctcttaaa atgcatcgtc tcctcaagga aaaactcccc caccatctgg ccattatcat
     3061 ggacgggaac ggacgctggg cggaaaagcg ctccctggac cgggtggaag gacatcggca
     3121 gggcgctgag tctgtgcgga cgatcgtccg tgcttcgcgg gagatcgggc tcccctatct
     3181 taccctcttt gccttttcct ctgagaattg gagccgcccc cgggcggaag tggacgcctt
     3241 gatgcttctc cttaagaatt acctatcctc cgaacttcag gagatgctgg ataggcaaat
     3301 ccggcttctg gccgtggggg agctttcccg tcttcccaaa gaggtacaga cgacccttta
     3361 tgacaccatg aagaaaacgg gtcgcgccac gggaatgacc cttactctcg ccctgagcta
     3421 cggagggcgg gacgacatcc tccaggccat ccgccggctg atgttccatt gccaggatcg
     3481 aagcctgact cctcaggaga tcaccgaaga ggttttctct aagtttttat ggacggccaa
     3541 ccttccggac ccagacctgc tcatccgcac cagcggagag atgcgcatca gcaatttttt
     3601 tctctggcaa ctggcctacg cggaaatcta tttcacccca accctttggc ccgatttttc
     3661 caaggaggag ttcatccaag ctcttctgag ctaccagaat cgagagcggc ggttcggttt
     3721 gaccagcgaa caaatccggg ccgaaggcgg gtaatggcat gcatctccaa cgctggatat
     3781 ccgggctgat tctcggcccg agtttgatcc tgttgattgt cttttccccg ccctggctat
     3841 tcctcctttt catcctcctg gtgattttcc tcgccttgcg ggagttctat gccctttgtt
     3901 taccagaggt ctctcccgga gaaaaagggg ccggattcat cctgggcttt cttgtggccg
     3961 tttcgcttta ctcccgggaa ccacgtggtt tcctttttgt gatccttttg gccttctttt
     4021 ttctctccat ccgggccctc ttccggccgg aagcgttccc ggcaagaatc gagacggtga
     4081 gcaagcacct cctgggtctt ttctaccttc ccttcctcct gagccatttc gtgctgatga
     4141 ggaatctgga gaggggaagg ctgtgggtcc tcttcactct ggtggctgtc tattttggcg
     4201 atacgctggc cttttatgtg ggccgggcct ggggaaagaa aaaaatggct ccccagatca
     4261 gcccggggaa gaccgtggaa ggagggttgg gggcggtcgc cgggtccgtc gctggagctt
     4321 tggtctccaa actgatcttt ttcccttcga tcccccttct tcatgccttg atcctgggag
     4381 cctccgtagg ggttatcgga caactggggg acctatggga atcccttctg aagcggagcg
     4441 tccaggtcaa ggactcggga gccttgatcc ccgggcacgg cggattgttg gaccggattg
     4501 acagcgtcct cttcgccgcc ccgctggttt attactatgc ctggcggatg ggagccggat
     4561 gaaaacaatt gccattctcg gctccacagg ctccatcggt gtcaactccc tggagatcat
     4621 tgccaagttt ccggaggaat tccgggtggt ggccctggcg ggcgggaaaa attaccggaa
     4681 gatggaagag caggtccgcc tctttcgccc cgatgtggcc tcggtcatgg acccagaggt
     4741 ggcccgccgg ctaaagcaga gtctatcttc cattcccacc aagattctct ggggaaacga
     4801 aggactcaat gccgtggcaa cccatcccca atcccaaatg gtcgtctccg ccctggtggg
     4861 aagcgtgggc ctcctgccca ccctggcggc gattcgggcg ggaaaatcga tcgccctggc
     4921 caacaaggaa gccctggtca tggccggaga ggtcataatg aaggaaatcc gggaaacccg
     4981 ggggaatttg cttcccatcg acagcgagca tagcgccatt ttccaggccc tggccggcca
     5041 tcgaaaagaa gagatcaaga ggatcatcct gaccgcttcc ggaggccctt tcctggacac
     5101 cccgccggag gagttgaaaa gggtcactcc ccgggaggct ttgaatcacc cccaatggcg
     5161 catgggcaag aaagtgaccg tggacagcgc ttccctgatg aacaaggggc tggaggtcat
     5221 cgaagctcac tggctgttcg gagttccggc ctcccggatc gaagttcata ttcatcctca
     5281 atccatcgtg cattctatgg tagaatatat cgacggttcg gtgatcgccc aaatggccct
     5341 tccggacatg agggggccca ttgcctatgc cctctcctat cctcagaggc tggacctccg
     5401 gcttccttcc cttaacctct tcgaggcagg gcccctttcc ttcaagccgg tggaccggga
     5461 gcgatttccg gccctggccc tggcgtacca ggcgctggaa acgggcggca ccatgcccgc
     5521 cgttctgaat gcggccaatg aggtagcagt agaagctttt ctggaagggc ggctggagtt
     5581 cctgaagatc cccagcgtga tccggaaaac catgaagaac catcaacccc tgcggctgaa
     5641 ttccgtggaa gatgtccttg aggcccattc ctgggcccgg gaggaagccc gggagactct
     5701 aaaccgaggt aacttgtgac gatcaccatt attgccacta tcattgtttt gggagtcctg
     5761 attttcgtcc acgaactggg ccattttctg gcggccaaag ggttccgggt gggggtgctc
     5821 actttctccc tgggatttgg tcccaaactt ttcggcaaaa aggtgggcga aacccaatac
     5881 caggtttcca tgatccccct ggggggctac gtgaagatgg tcggtgagga gccgggagag
     5941 aagattcccc cggaaatgat cgccaagtct ttttcccacc agcgagtcag ccggcgcttg
     6001 gggatcgttt ttgccggacc tttcttcaac tttctcttcg ctgtagtggc ctttgccgct
     6061 gttttcgtgg tgggaatgcc caccctactc cctgaggtgg gggaggttaa gcccgacctg
     6121 ccggcttacc aagcggggtt gcagaaagga gaccggatcc tggaagtcaa cgggtccccg
     6181 gccaagcgat gggaagacct ggccaaggtc attcatgaaa gcgcggatcg ccctctggtg
     6241 ttgaaggtgg aacgggagca aaaaaccttc caggtcaccg tgatccctca ggggtctctc
     6301 caaaagaata ttttcggcga tgatgttcag gtcggtttga tcggcatctc tccctcggga
     6361 aacaccttta tcgaacgggt ggaccctttc agcgcccttt accgggcggt ccttcaatcc
     6421 tggagagtga ccgaactgac ggtgatcagc atctataaaa tcatcgaggg gaagatttcc
     6481 gccaaaacca tcggcggccc catcttgatc gcccagttag ccggacagca ggccaaggcc
     6541 ggcgccctca gtctggtcat ttttatggcc gtcatcagta tcaatctggt catcctgaac
     6601 attctcccca ttcccgtcct ggatggatgg cacctcctca tttttctaat cgaatccgtg
     6661 gtgggcaggc cggtcagcct gaaactgcgg gagagggccc agcaggtggg cattttcatc
     6721 atcatcctaa tcatgctcct ggttttctac aacgacatta cccggattat cgggccagaa
     6781 tagagagatg ctcatcctag ccgttgaaac ctccacccgc acgggcagta tagctttagt
     6841 ggaagccctg ggggaggacc ctggggctcc ccttttgaag atcatcgggg agacgaccct
     6901 aaacctggcc gaaacccatt cttccaggtt gatgccggcc atcgaccgtc tccttcggga
     6961 aacttcacaa cccatccaaa caatccaagc catagctgtg ggtctcgggc ccggctcctt
     7021 caccggactg cgcatcgcca tctccacggt caaaggtctg gcttttgctc tccgggtccc
     7081 ggtcatggga gccccaaccc tcgacgtcct ggcgcaaaat ctctctttcg cttccatcca
     7141 ggtctgcccg gtcctcgacg cccgcaaaaa ggaggtctac gcggcccttt tccagggaaa
     7201 gggagaaggg cagttgacca aaatgagtga agactgggtc ttgagtccgg aagacctctg
     7261 ctcccggatt acggaaagaa ccattttggt gggaaatgga ggggaggttt acggggatat
     7321 tttcagggac cggctgggcc cccgggtcct tttcgcccac ccggaggatt ccttcccgcg
     7381 ggccagcaat gtggtgcgcc tcagcctgcc cgcctggcgg aaaggaaaga ctctggacct
     7441 cttcggtttc actcccatct acgtccgccg ctccgaggcc gagattcatt gggaccagaa
     7501 agcgcgcgga aaccctttat ccgagggtcg gtaaaagagg cttcgtccct tccgagaatt
     7561 ctttcgactg agcttggttc aaattctccg acgcggaatt cgaattttga tctttggtgg
     7621 ttcgctcgat cagccttctt ctttttccat ggcctcggga gaaacgaact cgcgtcgtac
     7681 cttccgcctt cgatccttcg ggaaatagag agtgaccgtg gttccctttt tcaggcgact
     7741 gtccacccgg attctccccc gatgctcttc catgacccgc cggacgaatg tcagtcccag
     7801 gccgacccgt ccaggatggg tcgagaagaa aggttcaaaa atcaggctga gattcttctt
     7861 cgggattccc accccccggt cagcgatgga tacccccaca ttatccccat catcaaagag
     7921 ggctaagtcc acgactttct tcccccctcc cttggacttg ggggggatcg cctggaggct
     7981 gttttccagc aggtgggtga gggctttgcc gatcaaatcc gcatcgatga agagccgtcc
     8041 attcccttcc atcccgcggg tgtccagtcg gaaggaagct tctttttctt gagccctatg
     8101 ggaaattctg gggagtacgt cttctaccag tttctgaatc ttctcccgac ggaaagcggg
     8161 ccgggggatt tgaacgtatt cctccacccg ttgaaggata ttttccagcc ggcccgtctg
     8221 tttcaaaatc tgcttgaggt acggcaggtt ttttgaggag cgcggccagt ttttggagag
     8281 acgtcgggta taccctccga tggaagcgat gggattgcgg aattggtggg cgatttctgc
     8341 aaccatcctc cccagtccgg cccatctttc catctccagc ctttccttct ccaagacctt
     8401 caaccgctgg atttcctgga agtagaaggt caggaaggct tctcccccct cctgaaagca
     8461 ggaggtggaa agatggacaa agattctcgt tccgtccttc tgcttgagaa ggacttcgcc
     8521 ttcaaatccg ttcttatacc gggtgagaaa aacgatattg ggaagaaagt aaagcaggtc
     8581 atcttccagg aagaggatcc tcaggtgttg accttccaac tcctcctgag tatagccgaa
     8641 gaatacctcc gcctgctgat tgacatagag aatccgggta tgagtatccg tcaggatgaa
     8701 ggaagccgga atggaatcaa gaactttcgg tttgcccttc aacatatccg ccatgaccgc
     8761 cccctcccgc tcttggttga ttgatcccag gataccctcg gcaaagcctg atcattgagg
     8821 ggcatcggtc ttattctacc cgggaccccg agaattggaa agaacatttt catgccttgc
     8881 ctgatttgag gtccaacttg cagctaaggg tatgctcgac tatttgcttg taagccgggg
     8941 cggttcttcc cgctgcggag tggaaaaagg agagtatccg gaatataata ggcacgccat
     9001 gatggaggat cgcccgaaaa tccgctgcgt catctacgac tgcgacgggg ttctgattga
     9061 ttccctgaat gccaaccgtt ggttttattc atttctctgt gcagccacag ggagatttcc
     9121 tttggccgaa gaggagctcc aatatgccca cacccacacg gtccacgaag cgatccatca
     9181 tatcttccgc gatgatcctg ggttggaaga gaaggccctg ggggttttct cgcggatgga
     9241 ccccgaggaa tccatgagcc ggttgaaatt ggagccccat cttattccca ccttggaagc
     9301 cctaaaagcc aaggggattc tccgggccat cagcacaagc cgaaccacga acgtggaggt
     9361 actcttaaga aggttctctc tggagcgtct cttcgacctc gtggttactt cccgggacgt
     9421 cctgaatccc aagcctcatc ccgaatctat cgaaaaggtc ctgcgggccc ttgtgattag
     9481 aaatgatgaa gcgctcttcg tgggggattc agagaccgac ggcagggcgg cgcgatccgc
     9541 cggggtgaaa ttcatcgcct acagaaactc ggggattgcg gctgatggct ttatcgagga
     9601 tcacccggac ctcttgaagt ttttgtgaaa aatcgcaaaa tcccccttcc tgcttctttt
     9661 gagactgtgt cgtaatcgga aaaatgggtt gtcattcccg ccaaagaggg aatccggtcg
     9721 cgattttatc caggtttgaa ccccctggat gcccgctttc gcgggcatga cgaattatga
     9781 cccagtctct ttccaaaggg cgaaaaagtt gaattttcct ccgtggatac cctcaaatcc
     9841 ctcctccctc tgaaaaaggg agggcgggag ggatttctgg gaaagccttt tcaagacgcc
     9901 aaagccttac gaaaaatcct ggttgccgaa aacgcctcgg ccaggaggtt tcttctcttt
     9961 ctaatccctc agacatagaa ggatcagtgg tgcgtaatct cgacccggtt gccatccggg
    10021 tctttgcaga agagataatc cgatccatcc atccgcttgg ccgggccatc gatgatttct
    10081 atccccttcg cccgcaattc ccggaccgtt tcctcgaagt tcgccacttc cagggctata
    10141 tggcgcatgg tatcgggaat atcttcctcg tatcccatga gatgcagctg gcaattcggc
    10201 cccacctggt accagtggcc cgggaagttg aagggcggcc gggcaatggt ctgcaaaccc
    10261 aagaacgttc cgtaaaacca tttgcatttt tccaggtttc tgaccacgat cccgatatga
    10321 ttgtattcct tcactttgat catcacgaac ctcctttttc atcagatagg attcttacca
    10381 aggagcccaa aactgagtgg acatattctc cggaaaatct cctctaatcc cgatgctttt
    10441 tacttattct atcaccgttc atcctgagcc ctttgattca ccgttcgacc tgagcttgtc
    10501 gaagggcgaa cgggacactc aggagatgct tgtcgaaggg tgcatcgcta caccgttcat
    10561 gcttcgacaa gctcagcatg aacggcttag tcctcagctt ccacgtgatc aaagaactga
    10621 ttagtaagga gaccccagat tcctctactt tttcctctcc cccatggctt tgacctgggc
    10681 ggtgagattg atgaggaagg gtctttcttc ctggttgatc aattttctct cgacgtagtc
    10741 ctcctggaag accttcttga ccaccgcatc ctggtttccc ccgttagctt tcagttcttc
    10801 ctctatcctt ttcccatacg cttgggtggc ctcgatggcc ttagggatca tcgcctgagc
    10861 atcctcggtg agcgcaaaat gatgccccag gagcagcagg tcgatcttca gggtcctgag
    10921 cttttctagg gatgaaaggt aatcatcgta gctggacagg aagaccggcc gtacgttgaa
    10981 ctgtcgatcg aaggttccta ccgcttcccc gcccaccagg gctttcagct tcgggatgta
    11041 gtaacaaacg gcatcccggg tgtgacctgg agtcgcaatg acccgaatgg cggttccccg
    11101 ttccagttct atttcttccc catcttccag gactcgatcc aggtccagtc cgcgaaaagc
    11161 cacatcttct tccccgacct gggacttgaa cttttcttcc atgggtctgc tcaggctttg
    11221 aatcagctgg atggcattcg gtctcttgaa tatgtcggcg gccagctttc cggccgctac
    11281 tttcaacccc gggatattcc ttttcaggaa aggggcggac ccggcatggt cgaaatgcga
    11341 atgggtgaga aaaaggtagc tgagccggtt gggatcaccc aaggttttct tcaggtcttg
    11401 gagataaaga gggcccatga cggtcattcc cgaatcgaag aggaccggcg atcctcccat
    11461 gaggaggtac gagggaagcg ccgaatttcc tagggcgtag aattcctcgg tgatttttcc
    11521 tttactctct atgatcatgt ttctttcctt gtcggattat tgggggtgac ccgccgggtt
    11581 gcccctactc cctctttacc atcttctcca acagctgctc ggcccggacc gggttgaagg
    11641 ccccgcactc gcagcacttg gccatgaggt ttgccgcctt ttgggaggtc aacctgcgca
    11701 gcttcacgta ctccaccatc ttgcggatga ggttgaagga gaccatccca tggccgcaga
    11761 gggtgttcac ttccagaatc tcccgctccg gcagccggtc cttggccccc caaaccccca
    11821 gagagtgctc cacgctatgg cgctcgatcc ctgccctgcg gcagcactcc cggacctcgt
    11881 ccagaagccc gctgatattg atggagattc ttaggtcggc ctggatcagc tcatcgatga
    11941 ctttccgcag ggtatccagg tcggtgaaga cggccgccgc cccctcgccg tcatttcgct
    12001 cggaaagaaa tttctggatc cccacatcct cctggagaat atttccaagg tagccgtcgc
    12061 ccatgttgac cggattatat ttcaaacaga tgcggatgaa ttcctggatc ttgggccctg
    12121 atccttggga gttgatccct ttggccatgg tgcagaaaat cacatagtca ttcttcaggc
    12181 tgctcggcgt tcccttccta tgcagggtgt tggtcatagg ttaatcctcc ttgaagaagg
    12241 gccggcccaa ccccaggttg gtctttccgt tgggagaaag agagtagccg gcctttctca
    12301 cccactcttc gtgaggagcg gaaccatccg gcaagcattt ggaggatacc cccacgctga
    12361 tcaccgtatc gacctccctc cctccccttt ccagagcgtg gaggatttcc gggatctttt
    12421 ccagttttac tttcatctcg attatcgcgg acataacttt ttcatccagg acctcttctt
    12481 tcatctttcc ggctcgggga tcgacaatca gctgggtgac gggattttgc ggctcaaaga
    12541 aaggctccag gcgggccatg gtcatggcta ttttctccac atcccggaaa tgagccccga
    12601 ttcccggccg gcccagctcc accacaattc ccgcctcccc cttgcggatt cttccggtca
    12661 cgtcattact tttgatctcc tcggtgcccc ggcccccgag gcccgttccc gggtggaccg
    12721 tggtcgggtc gctgaaggcc gcccggagat ttctcggcca ttccagttcc ggcggcgtca
    12781 gggccccggt ggggcaaacg tccacctccg ccaggtatcc caggcggaag gtcgaaagaa
    12841 ccttgcggat gactcgcaca aaccagggcg aataaccttc attccgcaaa acccggtaac
    12901 aggtattgca ctccacgcat tcctcctgat ttacctcgga ctttccatcc tccgccaggt
    12961 agatggctcc catagtgcag atcgtatggc agtttccaca accgacgcat ttctttttgt
    13021 caatttccat agaaacgaca acctcattca aatgcggaat ttggaatggg gaacgcgcaa
    13081 taaagcatac aggtcttccc caaaagggat gaatgcgggc taagaaccgc ggttctcccc
    13141 tgctaaagat ttttccccgg ctggattgat ttgccctgat aacctatcgg tcggataagg
    13201 taggttattt gaagcgaacc agcaattttc aaaattatat cctgaagggt ctttttcttc
    13261 aatttcgaaa tgcggatcaa tttgggctgg gcggaagggc cgagtgtgcc cggatgggtc
    13321 tcaaaaacgc ctgacgagct tcccccaatc gttgaaagcg cccgtcctcg cctctacccc
    13381 caaaatcccc cctacttccc cctgaaaagg cctgcaaaca aacccaagat tttgtactaa
    13441 attttcctaa atatgtgtta attaaaaaaa agtgttaaat atgtaatata tgttaaatgt
    13501 gtaaaatttg tgtaaaattt ttcttgacat acaaatgtct cttttgtaaa cttaaatccc
    13561 ccaaagcgag ttcttg
//
LOCUS       DASPRR010000546         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_135623, whole genome shotgun sequence.
ACCESSION   DASPRR010000546 DASPRR010000000
VERSION     DASPRR010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135623"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1073)
                     /locus_tag="VEL68_20185"
     CDS             complement(168..1073)
                     /locus_tag="VEL68_20185"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXZ38357.1"
                     /translation="MVPWKETAVSLWNNRLRFQLRAAWDILWEARGAFIQDGCMNLSA
                     ALAFYTILSLIPFLFLIVSAAGYLLGSSDEAQQMVIVFMDRFFPQASALILKEVQAIS
                     QRAGVLGWVGILSMIWTASVIFSSLEFAMGLVFRVEQRREFWKSKLLALSMIPASSVI
                     FFLSLSVTAFTGVRGSYSLQILGWDIGRSAFFSFLVGYLFPYLVLALTFTAVYRIIPN
                     TSITFRHAMAGGASCAFLFELAKHFFTWYIGRSSQYNVIYGSLEAIVILVIWVFYSSI
                     ILLFCAEVVSAYRRRDITLLEKAFL"
     gene            complement(1073..1711)
                     /locus_tag="VEL68_20190"
     CDS             complement(1073..1711)
                     /locus_tag="VEL68_20190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXZ38358.1"
                     /translation="MTRIYLIRHGTTEWNREEVFRGRVECPLNDTGQAEARAVAAYFQ
                     GIKIDKIYSSPLARAAETAAAIALGQGFEVLLDPAFTDLDFGEWQGHALKEVREKYPD
                     LYQTWRERPQDVTFPKGENLDQVRSRAWAGLLKVAQGNPDRTVLIVSHRVISKVLLCA
                     ALGLDNSHFWQIKQDTAAINCLEYSKGVFTVSLLNDTCHLKSLHLPGGRKDF"
     gene            1905..2324
                     /locus_tag="VEL68_20195"
     CDS             1905..2324
                     /locus_tag="VEL68_20195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXZ38359.1"
                     /translation="MFNKILVPLDGSKLAEKILPRVKKLVGPGQGEIHFIRVVVSYRI
                     DPKEEKEERERLMQEAWNYLEKVVSRLRKKRMRAYATVVYGKDAVQICDFARKNKFDL
                     IAMATHGRSGISRWALGSVADKVLGCSVVPVMLIRVL"
     gene            2343..2534
                     /locus_tag="VEL68_20200"
     CDS             2343..2534
                     /locus_tag="VEL68_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXZ38360.1"
                     /translation="MPKIPVVKQEECISCGLCAEVCPEVFRLNEKDLSEVYNPTGAPE
                     EKVQEAIDSCPVQCIVWEG"
     gene            2536..>3240
                     /locus_tag="VEL68_20205"
     CDS             2536..>3240
                     /locus_tag="VEL68_20205"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXZ38361.1"
                     /translation="MKGIPDFSEYRLPNGLQILVGPVHSLPMISFQVHFAAGSRYERP
                     GICGITHLFEHMMFKGTQQVGAEEFSRIIQANGGSLNAFTTTDNTSYYENLPADRLEL
                     VARLEADRLQNLLLTEENLETEREVVRSERKLRSVNSPFGLIIEQMVALAYEQHPYRW
                     PVIGWDSDLRSLTLEDCREYYRAHYAPNNAVVVIVGDVRVDEALKAVEKYFGPIPSQP
                     PPRLTITPEKPQRGEKR"
BASE COUNT          768 a          873 c          910 g          689 t
ORIGIN      
        1 atcggcgaat ccgtcgtcga taaagtgata ggtgcagaat gagaaaaggg agcggaagcg
       61 gttttggctt ttttggcgat tcaaaggttc ggcttagcct tcagcgtttg atccttggcc
      121 cttcatgatc cttgatccgt tcaccctatt ttccaactca tctttcccta aagaaaagct
      181 ttctccaaga gggtaatgtc tctccgccgg taagccgaga cgacctcggc gcagaaaaga
      241 aggataatcg atgaataaaa cacccagatt acgaggatga caatggcctc cagcgaccca
      301 tagatcacgt tgtactggga tgaccttccg atataccagg tgaaaaaatg cttggccagt
      361 tcgaagagaa aggcacaact cgcaccgccc gccatcgcat gccggaaggt gatggaggta
      421 ttgggaatga tccggtagac ggcggtaaag gtcagggcca gcaccaggta agggaaaagg
      481 taacccacca agaaactgaa gaaagcggag cggccgatgt cccaccccaa gatttggagg
      541 ctgtagctcc cccggacccc ggtgaaagcg gtcacggaaa gggaaagaaa aaaaatgacc
      601 gaggaggcgg ggatcatgga aagggccagc agtttggatt tccagaattc cctcctctgc
      661 tccacacgga agaccaatcc catggcaaac tccaaggagg aaaatatgac gctggcagtc
      721 cagatcatac tcaggatgcc cacccacccc aggactccgg cccgctggga gatggcctgg
      781 acttctttga ggatgagagc gctggcctgg gggaaaaacc ggtccatgaa aacaataacc
      841 atttgttggg cttcatcgga ggaaccgagc agataaccgg ctgctgagac gatcaggaaa
      901 agaaagggga tcagcgagag aatggtgtag aaggccagcg cggcggagag gttcatacac
      961 ccgtcttgaa tgaaggcccc gcgggcttcc cagaggatat cccatgctgc ccgcaactgg
     1021 aaacgcaatc gattattcca aagagaaacg gccgtctctt tccaggggac catcagaaat
     1081 ctttcctccc cccggggaga tgaagggact tcaaatggca ggtgtcgttc aagagggaaa
     1141 ccgtgaagac ccctttcgaa tattccaggc aattgatggc tgcggtgtcc tgcttgatct
     1201 gccagaaatg ggagttgtcc aggcccaggg ccgcgcagag cagaaccttg gagatgaccc
     1261 ggtgggagac gatcaagacg gttcggtccg ggttcccctg cgcgactttg agaagccccg
     1321 cccaagcccg ggaccggacc tggtccaggt tttctccttt cggaaaggtc acatcttggg
     1381 gtctctccct ccaggtttgg taaagatcgg gatacttctc cctcacctct ttcaaggcat
     1441 gtccctgcca ttctccaaag tccaggtcgg taaacgccgg atcaaggagg acttcgaacc
     1501 cctgcccgag ggcgatcgcc gctgcggttt ccgccgccct ggccagaggg ctgctataaa
     1561 ttttatcaat ctttatgcct tggaaatatg cagccacagc ccgagcttcg gcctgaccgg
     1621 tatcattcaa agggcattcc acccgtcccc ggaagacttc ttcccggttc cattccgttg
     1681 ttccgtggcg aatcaggtaa attcgggtca cccttcttct gctttcatct aacgttcctg
     1741 ggctgtcttt ctctcctgga gaagcattat tgcccaaggc agtgggactg tcaaccctaa
     1801 ctcttgccct tttacctcta tttgaagtga cattcatttt tcccgatgtt ataatttttt
     1861 atcctttcaa agaaggtttc ctcaacctag aaggaggagg gaatatgttc aacaaaattc
     1921 tcgttcccct ggatgggtcc aaattggccg aaaagattct cccccgggtg aagaaattag
     1981 tcgggcccgg tcagggggaa attcatttca tccgtgtggt cgtgagctac cggatcgatc
     2041 ccaaagaaga gaaagaggag cgtgagaggt tgatgcagga agcctggaac tacctggaga
     2101 aggtcgtttc ccgccttcgc aagaagcgaa tgagggctta cgccacagtg gtttacggca
     2161 aagacgcagt tcagatctgc gactttgccc gcaagaacaa gttcgacctc atagccatgg
     2221 ctacgcacgg ccgtagcgga atcagccgct gggccttggg gagtgtggcc gacaaggttc
     2281 tcggctgctc ggtggtcccg gtcatgctga tccgcgttct gtgatattgg aaaggaggga
     2341 gtatgcccaa gattcctgta gtcaaacagg aggaatgcat ttcctgcgga ctctgcgccg
     2401 aggtctgtcc ggaagtgttt cggttgaacg aaaaagacct ttccgaggtt tacaatccta
     2461 ccggcgctcc ggaagaaaag gtccaggagg ccatcgacag ttgtccggtc cagtgcatcg
     2521 tttgggaagg gtgaattgaa agggattccg gatttttccg aataccgtct gcccaacggc
     2581 ttgcagattc tggtcggccc ggttcattcc ctgcccatga tctccttcca ggttcatttc
     2641 gccgccggct cccgttatga gcggccgggg atctgcggga ttactcacct cttcgagcac
     2701 atgatgttca aggggaccca acaggtgggg gcggaagaat tctcccggat catccaggcg
     2761 aacgggggct ctctcaacgc cttcaccacc acggataaca cctcctacta cgagaatctg
     2821 ccggcggacc gtctggagct ggtcgcccgt ttggaagcgg atcggctaca gaatctcctg
     2881 ctgaccgagg agaacctgga gaccgaacgg gaagtcgtgc gcagcgaacg taaactccgt
     2941 tcggtcaaca gccctttcgg cctgatcatc gaacagatgg tcgcactggc ttatgaacag
     3001 cacccctacc gctggccggt catcggctgg gattcggacc tgagaagtct gaccctggag
     3061 gactgccggg aatactaccg ggcccattat gcccccaaca acgcggtggt ggtgatcgtg
     3121 ggagatgtcc gcgtggatga ggctctgaaa gctgtggaga aatactttgg ccccatccct
     3181 tcccaacctc ccccccgctt gaccatcact cccgagaagc cgcagcgggg ggagaagcgg
//
LOCUS       DASPRR010000547         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_942897, whole genome shotgun sequence.
ACCESSION   DASPRR010000547 DASPRR010000000
VERSION     DASPRR010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942897"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VEL68_20210"
     CDS             complement(<1..1257)
                     /locus_tag="VEL68_20210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXZ38362.1"
                     /translation="MRYKDLLQNRNFRWLLAALAIVAPLEVLSLFGLHLPLWAQIPLL
                     TALALVFGRGLLLSGVRSLFRVDFSNMNLLMTIAIVGAMYLGHWEEAGVIIVLFALGE
                     TLEDFGITKSQAALQDLVEKAPKTAQLKGKEGKVPVENVQVGEIIAVKPGDQIPLDGM
                     VTEGSSLVDEAAITGEPLPRSKYPGDPVFAGTINGSGYMEIRVTRQARDTTLSKIIQL
                     TYQSAERKSHSQRFIEKFARYYTPSVVSTSSLLVLIPVVLLGKPFDPWFIQALTLLII
                     SCPCALVISTPVTIFSAIGNATQRGALIKGGRFIEEMGRLKAIAFDKTRTLTRGEPVV
                     SDVFTFDNLSPKDVLACAAGIETFSEHPIARSILDQAEAENVEPHPFENFEAISGKGL
                     KGECMVCFDSHQCLGNIRFVSEEHQAG"
     gene            complement(1366..1779)
                     /locus_tag="VEL68_20215"
     CDS             complement(1366..1779)
                     /locus_tag="VEL68_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38363.1"
                     /translation="MNKSFSVLFLFTLILSLAGSAFSQTVYTGLIFDAQTLTFTPSAS
                     VKILDEDGREVYGSAYVSKDWADKYGIVSYVKDLAQAKANERVAGNPLVIKAIKVTGP
                     NNKDLVISNDDARRIRDLAKNLNFLDAGKVVIIVP"
     gene            2095..3327
                     /locus_tag="VEL68_20220"
     CDS             2095..3327
                     /locus_tag="VEL68_20220"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ38364.1"
                     /translation="MGKSLVSVVRDRGNVESAVREAVQLAGGLEGLISSKSKVLIKPN
                     IASRDRSGTGKITDVRVTEAVAKIVLERKPARMVIGEGSAVGYDFPDLQDTMMALEES
                     GTKALAEKLGVPVVDLNRDSHGEVKIPEALVMKTVKIARTVLESDVIVSIPVMKTHIR
                     SAVTLSLKNMKGVMPGAEKRKTHQLGLEQAIADLNAVVKPHFAVMDGLVGMEGLWEYP
                     DDCVRPGLVGASRDPVALDSVFARIMGVEVQEVMHLQYCQDRGLGMADPKEIEVIGIP
                     ILEVRRPFRPAFEVVKSRYPGLTVLAEKACTGCINEFISTLIYIRLAQQVDRLDGLTV
                     VLGEAPEALSAEKAVVIGKCARKMEGRFPFVPGCPPGVDEITQRVCEVCEIDVQLIFQ
                     KREELHRTIAGKAMQNSI"
     gene            complement(3345..>3672)
                     /locus_tag="VEL68_20225"
     CDS             complement(3345..>3672)
                     /locus_tag="VEL68_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38365.1"
                     /translation="LVYSAALLLEMLALFKLRLTRPDLPRPFRIPGGWPALLLVTLSM
                     GVFISACFIFSLLGENGGWIQAVIAVGMLLTGLLVYWISENFRKDSLPPAEWCRALGS
                     PERPPG"
BASE COUNT          867 a          935 c         1127 g          743 t
ORIGIN      
        1 tccggcttgg tgctcctcgg agacaaaacg aatgttcccc aggcattggt ggctgtcgaa
       61 gcagaccata cattcaccct tgagcccctt tcccgatatg gcctcaaaat tctcaaaagg
      121 atggggctcg acattctccg cttccgcctg gtccaggatg ctccgggcga tgggatgctc
      181 ggagaaggtt tctatcccag cggcgcaggc cagcacatct ttcggagaaa gattatcgaa
      241 ggtaaagaca tcggatacca cgggctcgcc ccgggtcagg gttctggtct tgtcgaaggc
      301 gatggctttc aaccggccca tctcttcgat aaaccgtcct cccttgatca gggcccccct
      361 ctgggtggcg ttgccgatgg ccgaaaatat ggtaaccggg gtagagatca ccagggcgca
      421 ggggcaggaa atgatcagga gggtcaacgc ctggatgaac cagggatcaa acggtttccc
      481 cagcaggaca acggggatta ggactaggag agaagaagtg ctcaccaccg agggggtgta
      541 gtaccgggcg aatttttcga tgaaacgctg ggagtgagat ttgcgctccg ccgactggta
      601 ggtcaactgg atgattttgg agagcgtcgt gtccctcgcc tgacgggtca cccgaatttc
      661 catatacccg gacccgttga tcgttccggc aaataccggg tctcccgggt acttgctgcg
      721 gggaagaggt tccccggtga tggccgcttc atccaccagg gagctgcctt cggtcaccat
      781 tccatccaga ggaatctggt cgccgggttt aacggcgatg atttccccga cctgcacgtt
      841 ctcgaccgga acctttccct cctttccctt cagttgggcg gttttggggg ccttctccac
      901 taggtcctgc aaggcagcct ggcttttggt aatgccgaaa tcctccagtg tctcccccaa
      961 ggcgaagagg acgatgatca ccccggcttc ctcccaatgt ccgagataca ttgcccccac
     1021 gatggcaatg gtcatgagga ggttcatgtt ggaaaaatcc acccggaata gactccgcac
     1081 cccgctgagg agcagacccc gcccgaaaac caaggctagg gcagtcagta gaggaatctg
     1141 agcccatagg ggcagatgga ggccaaagag agatagaacc tccagagggg caacgatcgc
     1201 cagagcggcc aaaagccatc ggaagttgcg gttttggaga aggtctttgt atctcataat
     1261 ttattataga cacagacggg gtctttttaa atatgagaaa aagcggtcgg agagacgggt
     1321 tccaggccgt ccttccgagg atcgaaccaa ggccaaagag cgaaattacg ggacgatgat
     1381 cacgaccttg ccggcatcca gaaaattgag gtttttggcg aggtcccgaa tcctgcgggc
     1441 atcatcgttg gagatgacca agtccttgtt gttcggccct gtcactttga tggccttaat
     1501 gaccagtgga ttcccggcaa cccgctcatt ggcctttgcc tgggccaagt ctttcacgta
     1561 gcttacgatt ccatacttgt ccgcccaatc cttggacaca tacgccgagc cgtacacctc
     1621 acgaccatcc tcatcgagga ttttcacgga ggccgagggg gtaaaagtga gggtttgggc
     1681 atcgaagatc aaccccgtgt agacggtctg cgaaaaagct gagccggcta aggaaaggat
     1741 gagggtgaaa aggaataaga ccgagaatga cttgttcatg tgcgtcctcc cttgattttt
     1801 ttgttcgggt tgttagggct ctccattttc ccgcgcagga atcataaaca atttttcgcc
     1861 ggtcgtctag gggaattttg gataacgaag agacgaaaga aaaggtaatg ggttaggggt
     1921 cataggaaga ggtcaatggc cagagaatag aaaaaaaggt acaggcccaa gaagccaggg
     1981 aattttttct atagataaat cagacgcttt tgggttacgc tacattgtcc catgtgcggg
     2041 tcgaataaga ttttaagatc aaggtgacgc ggttttcgaa gtggggggtt gacgatggga
     2101 aagtcgctgg tctcagtggt gagggataga ggaaatgtag aatcggccgt ccgtgaggca
     2161 gtccaactgg ccggcggcct ggaaggcctg atcagctcga aatcgaaagt cctgatcaag
     2221 ccgaacattg catcccgcga ccgatcgggg acggggaaaa taaccgacgt tcgggtcact
     2281 gaggcggttg cgaaaatcgt gctggaaaga aagccagctc ggatggtgat cggggaagga
     2341 tcggctgtcg ggtacgattt tcccgacctc caggacacga tgatggccct ggaggagtcg
     2401 ggcaccaagg cgctggcgga gaaactgggg gtgccggtgg tggacttgaa tcgggactct
     2461 catggggagg ttaaaatccc ggaggcgctg gtcatgaaaa cggtcaagat cgcccggacc
     2521 gttctggaaa gcgatgtgat cgtgagcatc ccggtgatga aaacccacat tcgctccgcg
     2581 gtgaccctaa gcctcaagaa catgaagggg gtcatgcccg gggcggagaa gcggaaaacc
     2641 caccaattgg gcctggaaca ggccatcgcc gacttgaatg ccgtggtcaa accccacttt
     2701 gcggtgatgg acgggctcgt cggcatggaa ggactctggg aatatcctga tgactgcgtc
     2761 cgcccgggtt tggtgggggc cagccgggac cctgtggctc tggacagcgt ctttgcccgg
     2821 atcatggggg ttgaagttca agaggtcatg catctccaat attgtcagga tagggggttg
     2881 gggatggccg atcccaagga gatcgaagtg atcggaattc ccattctgga ggtgaggcgc
     2941 cccttccggc cggccttcga ggtggtaaaa agccgttatc cgggccttac ggtgctggcg
     3001 gagaaagcct gcaccggatg catcaatgag ttcatcagca ccctcatcta tatccgtctg
     3061 gcgcagcaag tggacaggct ggacggatta accgttgtcc taggggaagc accggaagcc
     3121 ctttcggcgg agaaggccgt ggtcatcgga aagtgcgcgc ggaaaatgga agggcggttt
     3181 cctttcgtcc ccggttgtcc cccgggggtc gatgagatca cccagagggt ctgcgaggtc
     3241 tgcgaaatcg atgtccagct gatcttccag aaaagggaag agctgcaccg gacgatcgcc
     3301 ggaaaggcga tgcagaattc catctaagga cagccggtcg actttcaccc gggagggcgc
     3361 tccggcgatc ccagagcccg gcaccattct gcgggcggca gagagtcttt gcggaaattt
     3421 tccgaaatcc aataaaccag gagaccggta agcagcatcc ctaccgcaat gaccgcctga
     3481 atccagccgc cgttttctcc cagcagcgag aagataaaac aggccgatat gaaaacgccc
     3541 atggaaagcg tgaccagtag aagggccggc cagccgcctg gaatacggaa aggacggggg
     3601 agatcgggtc tcgtgaggcg cagcttgaac aaagccagca tttccagcag cagggcggcg
     3661 gaatagacca ag
//
LOCUS       DASPRR010000548         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_943264, whole genome shotgun sequence.
ACCESSION   DASPRR010000548 DASPRR010000000
VERSION     DASPRR010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943264"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VEL68_20230"
     CDS             complement(<1..238)
                     /locus_tag="VEL68_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HXZ38366.1"
                     /translation="MKKNRTKLVVIGGVAAGPKAAAKAQRCDPDMEIVIYQEEGEVSY
                     SGCGLPYYVGGVIPERKKLLIKTAEQFSQDGIQVL"
     gene            complement(307..777)
                     /locus_tag="VEL68_20235"
     CDS             complement(307..777)
                     /locus_tag="VEL68_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38367.1"
                     /translation="MISIEIPGWGNLEIENIIFDLNGTLAKDGELISGVKEKIELLSQ
                     LARIYILTADIHESAGWILQGVKAEWVRVKGEDSKSGKRNFLQSLKPEATVAVGNGNN
                     DQSILKECALGIVVLGGEGLSLAAMKDADLMVKDISDALDLLLKPKRLIATLRG"
     gene            complement(785..1624)
                     /locus_tag="VEL68_20240"
     CDS             complement(785..1624)
                     /locus_tag="VEL68_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ38368.1"
                     /translation="MKVRVTILSENLIGRAMGAGEHGFSAFIETDEGNYLFDTGGGRT
                     VVENSLALNKDLKSVKRIFLSHGHNDHTGGLPQVLNLTGPVDVHVHPDVFLDRMAIRK
                     EGGRESKRFAGMIYKRSYLEFLGARFILNSDFYEVERGMFLTGQVPRETAFEKNDPKL
                     FREVGGKTIPDTLPDDQSLILDTEKGLLLIFGCAHSGMINIINHVTQRMKKDHFLAIL
                     GGTHLDFLTPEQLEESIRSLKQMKVDRIGVSHCTGFRGACRLQQEFGERFFYGWVGSG
                     LEV"
     gene            complement(1706..2233)
                     /locus_tag="VEL68_20245"
     CDS             complement(1706..2233)
                     /locus_tag="VEL68_20245"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HXZ38369.1"
                     /translation="MKKASIEESAKKAYEKGKEYEKTYRGCSQCVIAALQDVLGVGSD
                     DVFKAATGLAGGTGLTCDSGCGAYMGGVLFLGSILGRERKNFSDPEGVRFKTHKLIRK
                     FRDQFIQEYGSVVCRDIQNRVLGRYYYLPDPQEFEKFHNAGAHDIHCPEVVGKGARWM
                     TEIILEEGLLSQQKT"
     gene            complement(2318..>2928)
                     /locus_tag="VEL68_20250"
     CDS             complement(2318..>2928)
                     /locus_tag="VEL68_20250"
                     /inference="COORDINATES: protein motif:HMM:NF015949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HXZ38370.1"
                     /translation="STFRDEGHPHKKGVKDIGHLTEKTALQLAQYALSDYLMEASIGM
                     AAINSLIEVEDRACVEKNAAEILLEKGEGKDIAVVGHFPFIPRLRERARNLWVFEQRP
                     QDGDLRPEEASKILPRCEVIGITGTSFINHTLEELLRWSQGKFVLLIGPTTPMTPFLF
                     DYGIHGLSGTMVVDPQEAFRFISQGATYQEIRGVRRVTMLRE"
BASE COUNT          616 a          879 c          696 g          737 t
ORIGIN      
        1 tcaggacctg gattccatcc tgggaaaatt gttccgcggt cttgattaaa agtttttttc
       61 tctccgggat gaccccgccg acatagtaag gaagaccgca gcccgaatac gagacctccc
      121 cctcttcctg ataaataacg atttccatat cgggatcgca ccgctgggcc ttcgccgccg
      181 ctttggggcc ggccgcaacc cccccgataa cgacaagctt ggtcctattc tttttcactc
      241 atttcctcct tcgatttatc atgtgcaaaa ccactctttc cctcctgaac ttgatagctc
      301 ggcgggttac cctcttaaag tggcgattaa cctcttgggc ttcaataaaa ggtcgagggc
      361 gtcggaaata tctttcacca tcaggtcagc atccttcatc gccgccaaag ataatccttc
      421 cccccctagg acaacgattc ccaaagcgca ctccttcagg atagattgat cgttgttgcc
      481 gtttcctaca gccacggtgg cttctggttt cagggattga agaaaattcc gtttcccgct
      541 tttactgtcc tctcccttaa ccctgaccca ctcggctttc acaccttgaa ggatccatcc
      601 ggccgactca tgaatatcgg ctgtgagaat gtatattctc gccaattgcg aaaggagttc
      661 aatcttctcc ttcactccgg aaatcagttc cccgtccttg gccagggttc cgttcaggtc
      721 gaaaattatg ttctcgattt ccaggttacc ccatccggga atctcgatgg agatcatttt
      781 ggcctcaaac ctccagtccg ctgcccaccc atccgtagaa aaagcgctcc ccgaattcct
      841 gctgcagccg gcaggctcca cggaagcccg tgcaatggga aactccgatc cggtccacct
      901 tcatctgttt cagactgcgg atcgattcct ccagctgttc cggggtcagg aaatccaggt
      961 gggttccccc gagaatggcg aggaaatgat cctttttcat gcgctgggtt acgtggttga
     1021 tgatgttgat cattcccgaa tgggcgcagc cgaagatcag gagcagccct ttctccgtat
     1081 ccaggatgag ggattgatca tccggcaggg tatcgggtat ggttttcccg ccgacctccc
     1141 gaaaaagctt cgggtcattc ttctcaaaag ccgtctcccg ggggacctgg ccggtcagga
     1201 acattcctct ctcgacttcg taaaaatcag aattcaggat gaaacgggcc ccgaggaatt
     1261 cgaggtaact ccttttgtag atcatcccgg caaagcgctt ggactcccgg cctccctctt
     1321 tgcggatggc catgcggtcc aggaaaacgt ccgggtggac atggacgtcc accgggccag
     1381 tcaggttcag gacctgaggg agaccccccg tgtggtcgtt gtgcccgtgg ctgagaaaga
     1441 tccttttcac gctttttaaa tctttgttca gagccaggga attctccacc accgtccgac
     1501 cacctcccgt gtcgaaaagg taatttccct cgtcggtttc gataaaagcc gagaacccgt
     1561 gctcccccgc acccatcgcc cgtccgatca aattctcact caggatggta acccgtacct
     1621 tcattccaaa atcctccact ttccagaata tttttaattg cttttatatc ttcatacccg
     1681 tgtatccgcg ttcatctgcg gcccattagg ttttttgttg ggaaagtagc ccttcctcca
     1741 ggataatctc ggtcatccat cgggcgcctt tgcccaccac ttcgggacaa tggatatcat
     1801 gggcccccgc gttatggaac ttttcaaact cctggggatc gggaaggtaa taatagcgtc
     1861 ccagaacccg attctggatg tcccggcaga ccacggaccc gtattcctgg atgaactgat
     1921 cgcgaaattt cctgatcagc ttgtgggttt taaagcggac tccctcggga tcagagaaat
     1981 ttttcctttc ccttcccagg atggaaccga ggaacaaaac cccgcccata taggcgccgc
     2041 agccgctgtc acaggtcagg ccggttcccc ccgccaggcc cgtggccgcc ttgaaaacat
     2101 cgtcagagcc cacccccagg acgtcctgca gagcggcgat cacgcactgg ctgcatcccc
     2161 ggtaggtttt ttcatattcc tttcccttct cgtaagcctt ttttgccgat tcttcaatgc
     2221 ttgccttttt cattcatttt ctcctttggt catgaatcta tttccccact gtcgggccca
     2281 ctttcattta caagttggat cggaaacgga agactactca ttcccgcagc atcgtcaccc
     2341 gacgcacgcc cctgatttcc tggtaagtag ccccctggga aatgaagcga aaagcttcct
     2401 gcggatcgac caccattgta cccgatagcc catggattcc gtagtcgaaa agaaaggggg
     2461 tcatcggcgt cgtggggccg atgaggagga cgaacttccc ttgggaccac cggagcaatt
     2521 cttccagagt atggttaatg aaggaagtac cggtgatccc gatgacctcg cagcgaggaa
     2581 gaattttgga ggcctcttcc ggccggaggt ctccgtcctg gggcctctgt tcgaacaccc
     2641 aaagatttcg cgccctttcg cgcaaccggg gaataaaggg gaaatggccc accaccgcta
     2701 tatccttgcc ttcccctttt tccaggagaa tctccgccgc atttttctcc acgcatgccc
     2761 ggtcctcaac ctcgatgaga gagttgatcg ctgccatccc gatggatgct tccatgagat
     2821 aatccgaaag tgcatactgg gccaattgca gggcggtctt ctccgtcagg tggccgatgt
     2881 ccttcacccc ctttttatgg gggtgaccct cgtcccggaa ggtggagg
//
LOCUS       DASPRR010000549         5321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1750679, whole genome shotgun sequence.
ACCESSION   DASPRR010000549 DASPRR010000000
VERSION     DASPRR010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750679"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..591)
                     /locus_tag="VEL68_20255"
     CDS             complement(67..591)
                     /locus_tag="VEL68_20255"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXZ38371.1"
                     /translation="MNRFIKTIEQISEWSGKGISFLIIPLTGVILYEILVRYLFKAPT
                     IWAHEVAQMIYGAYVILLGAYAMRKGIHVNVELLYGKFTPRTKAAVDLFTWILFYFFC
                     GLILVKGWEMAWESYQLGETEPTSFAPLVWPIKMTIPLGALLILLQGAVKSIRDVLTL
                     LWGAAPEKEQGHEH"
     gene            complement(632..1666)
                     /gene="dctP"
                     /locus_tag="VEL68_20260"
     CDS             complement(632..1666)
                     /gene="dctP"
                     /locus_tag="VEL68_20260"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ38372.1"
                     /translation="MKRLASVILVGVIAFCLAGTAFAQQPAAQKVIKWKFQSHWPSGS
                     TSFKPLKDFFDKEITRLTDGRLQITLYPASALVPTKEIFDSTRKGTIDGATASPIYWM
                     GIIPITAIASNCPMTFQEPWEGLYFHYIMGFEDMMKEAHAKFNILYYTEKIYPTALIS
                     KKPIRKIEDFKGMKIRSSGAIADFLRDLGAATNLIPGEELFLALQTGVVEAAHWGAAG
                     GALSMKFCEVAKYYIQPNLAMAGTDVIVINKEAFNALPKDIQTTLDLALRERAWRRTH
                     EYIMDEVDALNTMKRDYKVTVSTLSKEDQKKMVNVAMKQWDKAAAKDAASGKAAGMMK
                     DFLKKLGYID"
     gene            complement(1995..2828)
                     /locus_tag="VEL68_20265"
     CDS             complement(1995..2828)
                     /locus_tag="VEL68_20265"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXZ38373.1"
                     /translation="MITSKSNSKIKDIRLLKQVKHRQARGEYFIEGVRLVEEAFGSAQ
                     AVRQIVYSPRLENTPRGVSLLTLGRKRYPSIEWFYVSDEVLDSLIDTQTHQGILAVLA
                     KKEHSWEDLRRREGLILLLCELQDPGNLGTIFRVAEAGRAAGLVLNRGTLDPYNPKAV
                     RASMGSLFRLPFLRDRDAMESLRVFRSLGYRIWAAATGKGTCLWEADLTLPAVVLFGQ
                     EGAGLPEVLVEEADGILTIPMNAGVNSLNVAVSAGLVVYEAMRQQKVRSSLVQSKGPA
                     E"
     gene            complement(2906..3508)
                     /locus_tag="VEL68_20270"
     CDS             complement(2906..3508)
                     /locus_tag="VEL68_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXZ38374.1"
                     /translation="MVNSQVLVLNKSFLPVHVTTVKRAFCLLYAGIARAVNAQYETFD
                     YESWSRITIERNDETIGLVDRVIKIPRVILLLAYDRVPKRRVRFSRYNIFARDRNSCQ
                     YCGRRFSRNELNLDHVIPRSQGGTSTWENVVCSCHDCNRKKGGRTPAEARMRLIGQPR
                     KPAWTPPFNPSLQDVRFKDWVPFIDFMVDVSYWNTELLEE"
     gene            complement(3589..5037)
                     /gene="selA"
                     /locus_tag="VEL68_20275"
     CDS             complement(3589..5037)
                     /gene="selA"
                     /locus_tag="VEL68_20275"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HXZ38375.1"
                     /translation="MEPELRQKYLRALPAVDELLHHPQIQTLLKIHSRPLVVDSIRRV
                     LSEKRRAILQGVEEQAAAAASLTPDQWTAAVHETVGDSFRSSLRPVINATGVVLHTNL
                     GRAPLSEEALRNLVEIAGGYSNLEFNLKTGERGSRYEHVEEILCRVSGADSALVVNNN
                     AGAVLLALNSLAEGKEVIVSRGQLVEIGGSFRIPDVMRKSGARLVEVGTTNRTHLRDY
                     EQAITGETALLLKVHASNFRILGFTAEVSLKELAALGRERGLPVMEDLGSGCFVDLSP
                     HGIEHEPTVQETIQAGADVVTFSGDKLLGGPQAGILLGKQRFIDLLKKNPLNRALRID
                     KLTLAGLESTLRVYLDPERVMKVLPALSMLTCPLEDLERRARLFQRKLSRDLSSVCRV
                     RLKEENSQVGGGALPLQELPTRVVALRPLKLSATSLEERMRRNSPPVIVRVKEEEILL
                     DLRTVAKEEEAILLEAVKTALGNSELGRRNAE"
     gene            complement(5049..>5321)
                     /locus_tag="VEL68_20280"
     CDS             complement(5049..>5321)
                     /locus_tag="VEL68_20280"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ38376.1"
                     /translation="VSAPRYRQVAEAFGLDLKGKTDLQIGEMTVQAVEEFTKKTGLPQ
                     KLSQVSIPRENMLEVAKLALNDPGMKSNLRRVTDPQEIVPVLLAAW"
BASE COUNT         1099 a         1547 c         1477 g         1198 t
ORIGIN      
        1 aaggctagag gaaggcccag ggccagaaag aggagcagcg cgccgaaaag aagaagggta
       61 atcagctcaa tgctcatggc cttgctcctt ttccggagcc gccccccaca gaagcgtcag
      121 gacatcccgg atcgacttca cagctccctg gaggaggatg agcagggctc ccaggggaat
      181 ggtcatcttg atgggccaaa cgagaggagc gaaggaggtc ggttcggtct cccccagttg
      241 ataagattcc caggccatct cccagccttt caccaggatg agaccgcaga agaaatagaa
      301 gagaatccag gtgaagagat cgaccgccgc cttggtgcgg ggggtgaact tcccgtaaag
      361 gagctccacg tttacgtgga ttcccttcct catggcataa gcccccagca ggataacgta
      421 ggccccgtag atcatctggg ccacctcatg agcccaaatg gtcggggcct tgaacaggta
      481 ccggaccagg atctcataaa gaatgacccc cgtgaggggg atgatcaaaa aactgatccc
      541 cttcccggac cactcgctga tctgttcaat ggttttgata aagcggttca tggtcctctt
      601 tgacccttca aggtaagggg tatctccccc tctaatcgat ataccccaat tttttcaaga
      661 aatccttcat catcccggcg gccttccccg acgccgcatc cttggcagcc gccttgtccc
      721 actgcttcat ggccacattg accattttct tctgatcttc cttcgacagg gtgctcaccg
      781 tcaccttata gtcccgcttc atggtgttca gagcgtccac ctcgtccatg atgtattcat
      841 gggtgcggcg ccacgccctc tcccgcaacg ctaaatccag cgtcgtctgg atatccttgg
      901 gcagagcgtt gaaggcctcc ttgttgatca cgattacatc cgtcccggcc atggccaggt
      961 tcggctgaat gtaatatttg gccacctcgc agaacttcat ggaaagggca cccccggcgg
     1021 ccccccaatg cgccgcttct accactccgg tttgcagggc caggaacagc tcctccccgg
     1081 ggatcaggtt ggtcgccgct cccaggtctc tcaggaaatc agcgatggct ccggaagagc
     1141 ggatcttcat gcccttgaaa tcctcgatct tgcggatggg tttcttgctg atgagggctg
     1201 tgggataaat cttctccgtg tagtacagaa tattgaactt cgcgtgggcc tccttcatca
     1261 tgtcctcgaa gcccatgatg tagtgaaagt aaagcccttc ccagggctcc tggaaggtca
     1321 tggggcagtt ggaggcgatg gcagtgatgg gaatgatccc catccagtag atgggagagg
     1381 ccgtggcccc gtcgatcgta cctttgcggg tggagtcaaa aatctccttg gtggggacca
     1441 gggcggacgc cgggtagagg gtgatctgca gccgtccatc ggtaagccgg gtaatctctt
     1501 tatcaaaaaa atcttttagg ggtttaaacg acgtgctccc cgaaggccaa tgggactgga
     1561 atttccactt gatgaccttc tgggccgcgg gctgctgggc gaatgcggtt ccggctagac
     1621 agaaagctat gactcccacc aaaatcacgc tggccagtct tttcatgtct tacctccctt
     1681 ttctcggcct ttcatcaggc cggaatgtcc ttgaaacccc tgcagggcaa aacccggtca
     1741 agcccgtggg gtcccgcgct tcgcgggaca tcctgcccct gcaaccgcac cgggattacc
     1801 ggtggagcta ctttctccac acaaggctgc ggaaaaagcg taggaccttt ttatttcgaa
     1861 tcggggggaa tgtcaaggga aaacggcccg gaaaacagtt cggagaaact tagtccggag
     1921 ttcgagagaa aacaagggct gggggcttag gattatgcct acaggttcag ttatccgcgc
     1981 ttgtttttcg ccattcattc cgcagggcct ttactctgaa ctaaactact ccgaactttt
     2041 tgttgccgca tcgcctcgta caccacgagc cccgcggaaa cggcgacatt gagggaattc
     2101 accccggcgt tcatggggat ggtgaggatt ccgtctgcct cttcgacgag tacttctgga
     2161 agtcctgccc cttcttggcc gaacaaaaca acggcgggca gagtgaggtc tgcctcccac
     2221 aggcatgtgc ctttccccgt agcggcggcc catattcggt atcccaggga tcgaaaaacc
     2281 cgcagggatt ccatggcgtc tcggtcccgc aaaaagggaa gacggaaaag acttcccatg
     2341 gaggcccgga ccgctttcgg gttgtagggg tcgagggttc cccggttaag gaccagcccg
     2401 gcagccctcc ccgcttcggc gacccggaag atcgttccca ggttccccgg gtcctgcagc
     2461 tcacaaagaa gaaggatcag cccttccctc ctccggaggt cctcccagct atgctccttt
     2521 tttgccagga cggccagaat cccctgatgg gtctgggtgt cgatgagcga atccaggact
     2581 tcgtcgctga cataaaacca ttcgatggag ggatatcttt ttcgccccag ggtgagaagg
     2641 gagaccccgc ggggggtatt ttccaggcgg gggctgtaga cgatctggcg aactgcctgg
     2701 gcggaaccga aggcctcttc caccaggcgc actccttcga tgaaatactc tcctcgggcc
     2761 tgacggtgtt tgacttgctt gagaagacgg atatccttga tcttggagtt ggatttggag
     2821 gtaatcatag agacagtttc gagttccggg ttccgggttt cgagttcttt cttttaaccc
     2881 gaaactcgaa acttgaaacc cgaaactatt cttctagcag ctcggtattc cagtacgaca
     2941 cgtccaccat gaagtcgata aagggaaccc aatccttgaa gcggacatcc tgcagggatg
     3001 gattgaaggg aggggtccaa gccggtttgc gcggttgacc gatcagcctc atcctggctt
     3061 ctgcaggcgt tctccctcct tttttgcggt tgcaatcatg gcagctgcac actacattct
     3121 cccaggtgga ggttcctccc tgagagcggg gaatcacgtg atccaggttg agctcgttgc
     3181 gggagaatcg ccgcccgcaa tactggcagc tgtttcgatc gcgagcaaag atgttatagc
     3241 ggctgaaccg gacccggcgt ttagggaccc ggtcgtaagc caacaggagg atcactctcg
     3301 gaatcttgat tacccggtcg accaggccga tggtttcgtc gttgcgctcg atggtgatcc
     3361 ggctccagct ctcgtaatca aaggtttcat actgggcgtt caccgctctg gcgatacccg
     3421 cataaagcag gcagaatgcc cgcttgaccg tggtcacatg gacaggcagg aaggatttgt
     3481 tgaggaccaa tacctgggaa ttaaccataa ccaccccttc caatgcggat tgcagaattc
     3541 ggaatgcgga attcggtact cacaaaagag cctactatat gcattttttt attccgcgtt
     3601 ccgccttccc aattccgaat ttcctagggc cgtcttaacc gcctccagga ggatggcttc
     3661 ttcctccttc gcgacggtcc tcaaatccag cagaatctcc tcttccttta ctcggacgat
     3721 caccggcgga ctgttcctcc gcattctctc ctccagagag gtggcggaaa gtttcagcgg
     3781 gcggagggct actactcggg tcggcaactc ctgcaagggt aaggctcctc cgcccacctg
     3841 ggaattttcc tcctttaaac ggaccctgca caccgaggaa aggtccctgg aaagctttcg
     3901 ctgaaagagc ctggctctcc gctccaggtc ttccagaggg caggtcagca tgctgagcgc
     3961 tggcagaact ttcataaccc gctctgggtc caggtatacc cggagggtgg attccaaccc
     4021 cgccagggtc agtttatcga tccgcaacgc ccggttcaag ggatttttct tgagcaggtc
     4081 gatgaacctt tgctttccca ggaggatgcc tgcctgaggt cctcccagga gcttatcccc
     4141 gctgaaggta accacgtccg cccctgcctg gatcgtttcc tggaccgtag gctcatgttc
     4201 gatcccgtgg ggggagaggt ccacaaagca tccgctcccc aggtcctcca tcaccggcaa
     4261 tcctcgctcc cttcccagag ccgccagctc tttcagagaa acttcggcgg tgaaccccag
     4321 gatgcggaaa ttgctggcgt ggactttcag caggagggcc gtttctccgg tgatggcctg
     4381 ctcataatcg cgaagatggg tgcggttggt cgtgccaacc tccaccaggc gggcgccgct
     4441 tttcctcatc acgtcgggga tgcggaagga ccccccgatt tctaccagct gtccccggga
     4501 gacgatgact tctttccctt cggccaggct attcaatgcc aggaggacgg ccccggcatt
     4561 gttgttgacc accaacgcgg aatcagctcc cgagacccgg caaagaatct cctctacgtg
     4621 ctcgtagcgg gacccccgct ccccagtttt tagattgaac tccagattag agtagccgcc
     4681 ggcgatctcc accaagttcc ggagggcctc ctcggacagc ggagctcttc ccaggttggt
     4741 atggaggacc actccggtgg cgttgatcac ggggcgcaag gaggaccgaa acgagtcccc
     4801 cacggtttcg tgcacggcgg ccgtccattg atccggcgtt aggcttgccg ctgcggccgc
     4861 ttgttcttcg acgccttgca ggatagcccg gcgtttttcg cttaaaaccc tgcggatgga
     4921 atcgaccacc aggggccggg aatgaatttt tagaagagtc tgaatctgag ggtgatggag
     4981 aagctcatca accgccggga gggctcgcag gtatttttgt ctaagttcag gctccatatc
     5041 cccccgtgtc accatgcggc aagaaggaca ggaacaatct cctggggatc ggtaaccctg
     5101 cggaggttgg atttcatgcc cgggtcgttc agcgccaatt tggccacttc cagcatattc
     5161 tcccggggaa tgctgacctg ggatagcttc tgcgggaggc cggttttttt cgtaaattct
     5221 tccacggctt ggacggtcat ctccccgatc tgcagatcgg tcttcccttt caggtccagg
     5281 ccgaaagctt cggctacttg cctataacgg ggagcgctga c
//
LOCUS       DASPRR010000550        10547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_136333, whole genome shotgun sequence.
ACCESSION   DASPRR010000550 DASPRR010000000
VERSION     DASPRR010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136333"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VEL68_20285"
     CDS             complement(<1..408)
                     /locus_tag="VEL68_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ38377.1"
                     /translation="MALMNGEQYRNSLRKMKPLVYFLGERLTDPVDHPMVIPSQNAVA
                     MTYDLAHDPQYEDLMTVTSPLTGGKINRFGHIYQSIDDMVKKVKMARLVGQKTCTCFQ
                     RCPTMDTANSLFIVTKEMDKKLGTPYHERFLKFM"
     gene            complement(560..1471)
                     /locus_tag="VEL68_20290"
     CDS             complement(560..1471)
                     /locus_tag="VEL68_20290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ38378.1"
                     /translation="MPRIIDVHGHVFDSEGLKTIIHYRVQLSKYLYRKETTPEQLLKE
                     APTVEMMVKDFRANNMVGMPVAWDAETHLGGPKTSNDYIAGIVKKYPDVFLTGWGSVD
                     PWKGKLALQEAERCIKELKLIGLKFQQPTQQFHVNDPQFYPLWDLCQDLGAVIQLHTG
                     YSGLGTGLPGGGGIKLRYTRPIPDFDDLAADFPKLKIIAMHVGDPWVEELNAVTMHKG
                     NVYRETSGMYPRYFPENMKYEMNRRLQDKYMFGSEYNLFPLDQLIKQHQENGYREGFL
                     EKLFYKNAINILGENLERIGVNLKEWK"
     gene            complement(1486..2250)
                     /locus_tag="VEL68_20295"
     CDS             complement(1486..2250)
                     /locus_tag="VEL68_20295"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXZ38379.1"
                     /translation="MPVKGEGGIVTQAAKNLDAASLGKRTSQGQAKRRLILKVATEVF
                     AEKGFNESTISLIAKKAKIAEGGIYVYFKNKEDLLFSIPEERMENFLSGLHEHLEGIN
                     GALPRLRKIIWYHLNFYEKNKDYVLILLQNIQQNPRFNSTRAYRLIREFAKLVAQTIE
                     EGKKEGVIRPDIDSRLLRDAILGSIEHITIRGSILGRFPRITDAAGALYDFFVSGAQS
                     QTGMVTLHLREFLRMKKAILGEINPRKEKNLSSFKS"
     gene            complement(2576..3325)
                     /gene="fabG"
                     /locus_tag="VEL68_20300"
     CDS             complement(2576..3325)
                     /gene="fabG"
                     /locus_tag="VEL68_20300"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HXZ38380.1"
                     /translation="MRLKDKVAIVTGAGRGIGEAIALLFAEEGANVVVNDVNEADVKS
                     TAEEIKGRGRQARSVIGSVTVQGVVQRMVDTAVQEFGTLDILVNNAGIIRDARLHKMT
                     EEQWDRVIEVNLKGAFLCTQCAARVMREKRYGKIINLSSAMWKGNPGQLNYSAAKAGI
                     IGLTRTAAKELAPKGINVNAIAPGLIWTDMLKSTPPAILEQMQNNLATMVPLNRLGLP
                     RDVANVALFLASDESSYVTGEVISCDGGLII"
     gene            complement(3821..5758)
                     /locus_tag="VEL68_20305"
     CDS             complement(3821..5758)
                     /locus_tag="VEL68_20305"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ38381.1"
                     /translation="MEPFRNLFQPIRIGKMELKNRIVMLAMTTGYGESDETVSDRLVN
                     FFAERAKGGAGFIIVPFSPVRAGSPVEPGLYEDRFIPGATRLTERIHAFGAKIASQLI
                     TSYHVILGSGIPEVVGPSPVVNLMMRCTPRPLTVDEIHGIVEEYAKSARRVREAGFDA
                     VEILVGAGYLLNRFLSPVSNKRQDQYGGSLENRMRITLEIIASVRKAVGEGFPISVRL
                     NVEEQMGGGHTIEDSKGVAQILEKAGIQVINMYTGWHESPVPTVQPSLPKGAFAHLAE
                     KIKSGVRIPVVATNRINDPWVAEKILAEGKADLIGMGRALLADPEFPKKAREGRVEEI
                     IPCIACSNCLTEVMGAYRQWGKPVATFCTVNPVAGKEGHYIPHPAPKPKKVMVIGGGP
                     AGLEAATIAAMRGHRVTLYDRGEELGGRLLLAAIPPYKSEMKALAQSLSARARKAGVR
                     IKLHSEPGRESIQEEKPDALVLALGSTPRLPDIPGSRGQNVVFAEEVLAGSKSVSGSV
                     LVVGGGVVGCETAEFLIEQGKGVTQVTVLEMLDRMANDMSPTYRPFFLARLKRAGIKM
                     MTGARVEEITEKGVRVKQGDGSTWVEGNAVVLAGGSMANRQLVQELEGIVPEVHVAGD
                     CISPRMVKEAMEEGFLAGWKI"
     gene            complement(6100..7488)
                     /locus_tag="VEL68_20310"
     CDS             complement(6100..7488)
                     /locus_tag="VEL68_20310"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXZ38382.1"
                     /translation="MKGFIRNWAEKWHFGMEKVMPDSFIFAALLTFIVFIMAFVVVGA
                     SPVQIVECWYRGFWAYLLFSMQMVILLIFGYSLAITRIGIKAIDAVTGIAKTPPQAVA
                     LVCAAAALLSVVNWGLGFIGGIFLCIGAARRVKGVHWPLLVASAYIGAISTDTWGVGL
                     TEPLLLNSPGWGWAPDMVQSIEKEIGQKLAPMSFDKTIWAPASLVGLVLTPILATIVC
                     YLMHPTPERTKTISPQALEKLSAQSQFKLERPKEMVLADKLNWSRTIWILLCIMTFAA
                     ILLWFQGKKFAQVDLNMYNFIFIGIAMLLHENLARFVESIKVAAGAAFGIIMQFPFYA
                     GIFGIMAYTGLLKVIAGWFVAISSPFTYPLVTMISSGIINMFIPSSGGIWMVQGPIMV
                     LAAHKLGVGFNRVVMAFSAGEVLTNVIQPFWALPLLGATGTEMRTIMGYCMLACLFEF
                     ILVALIYLFLPM"
     gene            complement(7643..8569)
                     /locus_tag="VEL68_20315"
     CDS             complement(7643..8569)
                     /locus_tag="VEL68_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXZ38383.1"
                     /translation="MSKVIITAAITGSIHTPTMSPYLPITPDQIAENAVQAREAGAAV
                     VHIHVRNSETGQPTPDLELFRKVLGKIKSKSDLVICTTTGGGFGQTPEQRVAVVPTFK
                     PELASFNMGTMNFGLFPVLEKMKEFKFPWEKQFLEATEDVIAFNSFKSMKVFFEKFNE
                     NQTKPELEVYDAGMINSVAYFIEKGLLKTPVYIQFVLGILGGMAATVQNLVILYESAC
                     RQIGEKNFLWSVCAAGISQMPMCTTSLLMGGNARVGLEDALRVSKGKLAKNNAELVEK
                     IVRIAGEFGLEPATPAEARKMLGLKGLDKVAY"
     gene            complement(8828..10006)
                     /locus_tag="VEL68_20320"
     CDS             complement(8828..10006)
                     /locus_tag="VEL68_20320"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXZ38384.1"
                     /translation="MAALKGVRILDLTLTLPGPYCTHLLADFGAEVIKVENPEVGGDW
                     FRTENPSIKGWGIRFLDLNRNKKSITLNLKSPKGKEIFFALARTSQVIVEGFRPGVVE
                     RLGIDYRRMKEINSRIVYCSISGYGQDGPYRLLAGHDLNYMGYSGILDPQGYADRPAA
                     LPSVFVADYSGGGLMAAVGILVALMAAQREGKGQWVDISMFDGVVGLQHAALAELLST
                     GRSPKRGEFWGAGNIPCYSLYETKDGKSISLGPLEPHFWANLCRALGREDFIPHQWTE
                     GEKRQEIYAFLRKTFRQKDRDEWVALFQGQDVCLGPVKDMGETLRDPQVLHRQMIVDV
                     EHPQAGRVKQIGIPIKLSETPGEINHPAPALGQHTEELLKELGYDRQAIEGLRREKAI
                     "
     gene            complement(10135..>10547)
                     /locus_tag="VEL68_20325"
     CDS             complement(10135..>10547)
                     /locus_tag="VEL68_20325"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ38385.1"
                     /translation="SGVEGGETEAGSYFVNQLLGNVCKLNVGTLPFEMARLAVDVTGG
                     ILGTMVSEKDLKSPEIGKYVEKYMRGLPEVPTEHKMRLIYLIQNILFGINSVGYVVES
                     MHGAGPPAAQKSSLNRLVDFEEKKDLAKEISGIP"
BASE COUNT         2259 a         3080 c         2649 g         2559 t
ORIGIN      
        1 catgaacttc aaaaagcgtt catgataggg ggtccccagc tttttgtcca tctctttggt
       61 gacgatgaaa agggaattgg ccgtgtccat ggtggggcag cgctgaaagc aggtgcacgt
      121 tttttgcccc accagcctgg ccatcttcac ttttttcacc atatcatcga tgctctggta
      181 gatatgaccg aaccggttga ttttccctcc ggtcaggggg gaggtaacgg tcatgaggtc
      241 ctcatattga gggtcgtggg ccagatcgta ggtcatggct acagcattct gggaggggat
      301 caccatagga tggtccacag ggtcggtaag acgttctccc aagaagtaga ccaagggttt
      361 catctttcgt aaactgttcc ggtactgctc tccgttcatc aaagccatac aatcctcccc
      421 tgcacgatta aattcaatcc cccgggaggg ggatgttcct aggtttcctt tccgttcgag
      481 tttctttcat ttttcccggc atccctcctg cgctgaggat ttgagaccgg ggctcctgcg
      541 gccggatgag aaggcccggc tacttccatt ctttcagatt gaccccgatc cgctcgagat
      601 tctctcccaa gatgttgatg gcatttttat agaaaagctt ttccagaaat ccctctcggt
      661 agccattctc ttgatgctgc ttgatcaact gatccagggg gaagaggtta tactcggacc
      721 caaacatata tttgtcctgg aggcgccggt tcatttcgta tttcatgttc tcaggaaagt
      781 aacgagggta catgcccgag gtctcccggt agacgtttcc tttgtgcatg gtgacggcgt
      841 tgagttcttc cacccaggga tctcctacgt gcatggcgat aatcttcaat ttcggaaagt
      901 cggccgccaa atcgtcaaaa tctggaatgg gcctggtata gcgcagcttg atccccccgc
      961 cccccggaag gccggtgccc aacccggaat agccggtgtg aagctggatc accgcgccca
     1021 ggtcctgaca gaggtcccag agaggataaa actgggggtc gttgacgtgg aactgctggg
     1081 tgggctgctg gaatttcagg ccgatgagtt ttagctcctt gatgcatcgt tccgcctcct
     1141 gcagcgccag cttccctttc cagggatcta ccgagcccca cccggtgagg aacacgtccg
     1201 ggtatttctt gacgatgccg gcaatgtaat cattgctggt cttgggaccc cccaaatggg
     1261 tctccgcgtc ccaggcgacc ggcatgccca ccatattatt cgcgcggaaa tcttttacca
     1321 tcatttccac ggtgggggct tctttgagga gttgttcagg agtagtttcc ttccggtaaa
     1381 gatacttgga aagctgaacc cggtagtgga tgatggtctt taatccttca ctgtcgaata
     1441 catgtccgtg gacgtcgata attcttggca ttttccccct ccttgttagg atttaaatga
     1501 cgacaggttt ttttctttcc tggggttgat ttcgcccagg atcgctttct tcattctgag
     1561 aaattcgcgc agatgcaggg tgaccatgcc cgtctgagat tgggcccccg agacaaagaa
     1621 atcatagagc gccccggcgg catcggttat cctcgggaac ctgcccaata tagacccccg
     1681 gatagtgatg tgttcaatgg accccaggat ggcatcccgg agcagcctgg agtcaatgtc
     1741 cggccggatg actccctcct tttttccttc ctcgatggtt tgagccacca gcttggcgaa
     1801 ctcgcggatc agccggtacg cacgggtgga attgaaccgg gggttttgct ggatgttttg
     1861 aagaagaatc agcacgtaat ctttattctt ttcatagaag ttgagatgat accagatgat
     1921 cttacgaagt ctgggcaggg ccccgttgat tccctctaaa tgctcgtgga ggccagagag
     1981 aaaattttcc atccgctcct cgggaatgga gaagaggagg tcttctttgt tcttgaaata
     2041 gacatagata cctccttcgg caatcttggc tttcttggcg attagggaga tcgtggattc
     2101 gttgaagccc ttttcggcaa agacctccgt cgccactttc aagatcagtc gtcttttcgc
     2161 ttgtccctgg ctggtcctct tcccgagaga ggcggcatcc aaattcttcg cggcttgcgt
     2221 aacgatccct ccttctcctt tcactggcat caggtgccca ggggtctggc tgagccaagg
     2281 caaaattgaa cttgaatatt actcaaattt aaaatcatgt caagaataat ttttctccgc
     2341 caagaaaata ttttgatcgg attgatatta aatatatcat aaaatatcaa ttacttacga
     2401 attattttct ccttgaccat attggtcatc gattctactg agtgaaattc atgttttttg
     2461 tatctggatt ggcccttccg attaaggagc gggtcccttc ggttgaggtt tccaccgcgg
     2521 cccccctcct ccttcttgcc ggcaataagc gaaggtaacc ttcttgagag ctttcctaaa
     2581 tgatcaaacc gccgtcgcag gaaatcactt cgccggtcac ataggagctt tcgtctgagg
     2641 ccaggaagag agctacattg gccacatcgc gcggcaagcc cagccggttc agggggacca
     2701 tagtcgccag gttgttctgc atctgttcga gtatggccgg aggggtggat ttgagcatgt
     2761 cggtccagat cagtcccggg gcgatggcat tgacgttgat ccctttgggc gccaattctt
     2821 tggctgccgt tctggtcaat ccgatgatgc ccgccttggc agccgaataa tttagctgcc
     2881 cagggtttcc cttccacatc gccgaggaga ggtttatgat cttcccgtag cgtttctccc
     2941 tcatcacccg ggccgcgcac tgggtgcaga gaaatgcgcc ttttaaattc acctcgatga
     3001 cccgatccca ttgctcctcg gtcatcttat gcagcctggc atcgcgaatg attcctgcgt
     3061 tgttaaccag gatatccagg gttccgaatt cctggactgc cgtgtcgacc attctctgaa
     3121 cgacgccctg aacggtaaca ctgccgatga cggatctagc ctggcggccc cggcctttta
     3181 tttcttccgc cgtggatttt acatcggcct cattcacatc attgaccact acgttagctc
     3241 cttcttcggc aaagagcaga gcgatcgctt ccccgattcc tcgcccggcc ccggtcacaa
     3301 tcgccacttt gtctttcaat ctcattttcc ccattctcct tttcttccat cttcccgcaa
     3361 aacatcccta aagaaagacg acggaaacct cactgcaaac taagagctcg aacccccgcc
     3421 gctctgccgg gggtccgtct caagcggatt gacatttccg gtatgcctcg gttgtggccc
     3481 gggggcaagg gccttctggg caaaaagcat cggccgggtt ggccctcttc ttataggctt
     3541 ggaccggctt ccgcaggatg cggcgggctc aacgggcgcc cggccggtcg cctctacaac
     3601 ggaataatct tgcccttggg caaagaaggg ttggcccgcc cggtccatga aataaaagct
     3661 gcctcatgat ttttgcccag aaggtccttg cccccgggcc tggcacccgg gtgggccacc
     3721 tttaggtgac gctcgtttca atcggttttg agcggttccc tgtttttcat accttcggtt
     3781 gtgccggagg tcatgatttc cagatgatcc aaaaaattga tcaaatcttc catcccgcca
     3841 ggaatccctc ttccatcgcc tccttgacca tgcgggggct gatgcaatcc cctgccacgt
     3901 gaacctccgg gacgatccct tccagttcct ggacaagctg acggttcgcc atcgaacccc
     3961 ctgctaggac cacggcattc ccctcgaccc atgtagaccc gtcgccctgt ttgaccctta
     4021 cccccttttc cgtgatttct tccacccttg cgccagtcat catttttatt cctgcccttt
     4081 tcaacctggc caggaaaaag ggccggtacg taggagacat atcattcgcc atacggtcca
     4141 gcatttcaag aacggtgacc tgggtgacgc cttttccctg ctcgatgagg aactcggccg
     4201 tttcacagcc cacaactccc ccgccaacca ccaaaacgga cccgctcacc gactttgatc
     4261 ccgcaaggac ctcctcggca aaaacgacat tctgtccccg ggacccaggt atatccggga
     4321 gccggggggt tgaccccaaa gccaagacca aggcatccgg tttttcttcc tggatgctct
     4381 ctcgcccagg ttcgctatga agcttgattc tcaccccggc ctttcttgcc ctcgcggata
     4441 ggctctgcgc cagggccttc atctcacttt tataaggtgg tatggcagct aagagaagcc
     4501 gtccccccaa ttcttcccct cggtcataga gggtcacccg gtgacctctc atggccgcga
     4561 tggtcgcagc ctccaagcct gccggaccgc cgccaatcac catgaccttc ttgggcttag
     4621 gcgccgggtg cgggatataa tggccctctt ttccggcgac gggatttact gtgcagaaag
     4681 tcgccaccgg tttgccccat tgtcggtagg cccccatgac ctcggtaaga caatttgaac
     4741 aggcaatgca cggtataatt tcctcgaccc ttccctccct agccttcttg ggaaattcgg
     4801 gatcggcaag taatgccctg cccatgccaa tcaggtcggc cttgccttct gccaatattt
     4861 tttccgccac ccaagggtca tttatgcgat tggtagcgac gaccgggata cgaaccccgc
     4921 tcttgatttt ctcggcgagg tgcgcaaaag ctcccttggg aagagaaggc tggacagtag
     4981 gcacgggaga ctcatgccag cccgtgtaca tattgatcac ctgaataccg gctttctcca
     5041 ggatttgcgc gacccccttc gaatcctcga ttgtgtgtcc tcctcccatt tgttcttcca
     5101 cattgagacg cacgctgatc gggaaccctt cgccgaccgc ttttcttacg cttgcgatga
     5161 tctccagggt gatcctcatc cggttttcaa ggctgccccc atattgatcc tgacgcttat
     5221 tgctaaccgg agagaggaat cggtttagga ggtaacctgc gccgacgagt atttctactg
     5281 cgtcgaagcc cgcctcccgg accctccgcg ctgatttggc gtattcttcg acaatcccgt
     5341 gtatctcatc gacggtcagg ggccggggcg tacagcgcat catcagattc acgaccggcg
     5401 atgggccaac cacttcgggt atgcctgaac ccaatatcac atgataggag gtaataagct
     5461 ggctggcgat tttggctccg aaagcatgaa tcctctcggt caaccgcgtg gcccccggaa
     5521 tgaagcggtc ctcgtataaa cccggttcaa cgggcgagcc tgcgcggacc ggcgaaaaag
     5581 gcacgataat aaacccggct cctcccttgg ccctttcggc aaaaaagttc accagccggt
     5641 cggagacagt ctcatctgat tccccatacc cggtggtcat ggccagcatc acgatccgat
     5701 tcttcagttc cattttgccg atccgaatcg gctggaataa atttctgaac ggctccatac
     5761 ccggtcctcc ggatttctta agcctaaaac tttaccaaga tatcttagaa cttgtccgca
     5821 aataatattc ccccctttgc aaaggggggt tggggggatt ttcctaaagc gatctaaagc
     5881 aaatccccct atgtccccct ttttcaaagg gggatttaaa cgcagacctt tatttaagga
     5941 taagctctta ggcgttcagt gccaaactgc tcgccatccc gcgtctcgcg ggaataggaa
     6001 gagaatatgc ttcatctggg ctgtaggggc atcgcgctcc cgcgagacat cgtgccccta
     6061 caagcgcagc gtatcgcctt tgaggcctcc gaagtaaccc tacatgggca agaacaggta
     6121 gatcagggcg accaaaatga actcgaacag acaagccagc atacagtatc ccatgatcgt
     6181 tctcatctcc gttcccgtag ctcccaagag cgggagagcc cagaagggtt gaatcacatt
     6241 ggtcaaaact tctccggcgg aaaaggccat caccactcgg ttgaaaccca ctcccaattt
     6301 gtgggccgcc agaaccataa tcggtccctg gaccatccaa atcccacccg aggaagggat
     6361 aaacatgttg atgattcccg agctgatcat cgtcacgagc ggataggtga agggactgga
     6421 aatggccacg aaccatccgg caataacctt gagcagaccc gtgtaggcca tgatgccaaa
     6481 gatcccagca taaaagggaa attgcatgat aatcccaaat gccgcgccag cagccacctt
     6541 tatggattcc acaaagcggg ccagattttc atgcagcagc atggcgatgc cgatgaaaat
     6601 gaaattgtac atgttaaggt ccacttgcgc gaacttcttc ccttgaaacc aaagaaggat
     6661 agcagcaaaa gtcatgatgc acagcaggat ccaaatggtc cggctccaat tcagcttgtc
     6721 agccagaacc atctccttgg gcctttccag cttgaactgc gattgagcgg ataacttttc
     6781 cagggcttgc ggagagatgg tcttcgtccg ctctggagtc gggtgcataa ggtagcatac
     6841 gatcgtggca agaatggggg tcaggactag gccaacgagg ctggctggag cccaaatcgt
     6901 cttgtcgaaa gacatgggag ctagtttttg cccgatctct ttctcgatgg actggaccat
     6961 atcgggtgcc caaccccacc cgggactatt gaggagcagg ggttccgtaa gccccacgcc
     7021 ccaggtatcg gtggagatcg ccccgatgta cgcgctggct accagcaaag gccagtggac
     7081 cccttttact ctccgggcgg ccccgatgca caaaaagatc cctccgatga accccagccc
     7141 ccagttaacg accgaaagaa gggccgccgc cgcgcaaacc agggccacag cctggggggg
     7201 ggttttggcg attccggtga ccgcatcgat ggctttaatt ccgatgcggg taatggccag
     7261 agaataacca aagatcaaaa ggatgaccat ttgcatggaa aagagcaggt acgcccagaa
     7321 tcctctgtac cagcattcaa cgatttggac tggagatgcc cctacaacga cgaaggccat
     7381 tatgaaaacg atgaaggtga gcagggctgc aaagataaag gagtctggca tcaccttctc
     7441 cattccaaaa tgccactttt ccgcccaatt ccggataaat cctttcatta caagcctcct
     7501 ttcagagatt tattctcagc agggtgccaa aatgcacgga aaggcatctt tcctcggacc
     7561 atggaacgat agcgcttgaa ggtcctcctc ctgtcctcct tttctccctc cttaccagtt
     7621 cccccttttc ctctccttcc cttcaataag cgactttgtc caggcccttc agcccgagca
     7681 tcttccgcgc ctccgccggc gtagcgggct caagcccgaa ctccccggcg atgcgcacga
     7741 tcttttccac taattcggcg ttgttcttgg ccagtttgcc cttgctcacc cgcagcgcat
     7801 cctccaatcc cacgcgggcg ttgcccccca tgagcaggga cgtggtgcac atgggcatct
     7861 ggcttattcc cgcggcgcag accgaccaga ggaaattctt ttccccgatc tgcctgcagg
     7921 ccgattcgta gaggatgacc aaattttgaa cggtggcggc catgcctccc aggatgccga
     7981 ggacgaattg gatgtaaacc ggggttttca aaaggccctt ttcgatgaaa taagcgacgc
     8041 tgttgatcat cccggcatcg taaacttcca gctccggctt ggtttggttc tcgttaaatt
     8101 tttcgaaaaa tactttcatt gatttgaagc tgttgaaggc aatcacgtct tcggtggctt
     8161 ccaggaactg tttttcccag ggaaacttga attccttcat cttctccagg acggggaaga
     8221 ggccaaaatt catggtgccc atattgaagg aggccagctc cggcttgaaa gtgggaacca
     8281 cggccaccct ttgctctggg gtctggccaa accctcctcc cgtggtggtg cagatgacca
     8341 agtcgctctt gctcttgatc ttgcccagca ctttcctgaa aagctccaaa tccggggtag
     8401 gttgcccggt ttccgagttc cgcacgtgga tatgaaccac cgccgccccc gcctccctgg
     8461 cctggacagc gttttccgct atctgatccg gagtgatggg aaggtaagga ctcatcgtcg
     8521 gcgtgtggat cgatccggta atggccgctg tgataatcac cttgctcatg aatgcattcc
     8581 tcctttggta atcattcttc caagattttt atttcagcag taggctttga cgatcttttg
     8641 gatacatccg gggtcgagac tcccgggctc cgccgcatgc ccgcggggga ggacggcaat
     8701 caggatatcc ccattccttc gggtagatgg ttaaaaggac aggttggagc attgatccaa
     8761 tcagttgtgg agctgtgatt ttgttgtctg gaaagccgag ccctggccgc tacactttga
     8821 tgacgattca aatcgccttt tcccgccgga gtccttcgat ggcctgccgg tcatatccta
     8881 actctttcag aagctcctcc gtatgctggc ccaaggcagg ggcgggatgg tttatttccc
     8941 cgggggtctc ggaaagcttg atgggaatgc cgatctgctt cacccggccc gcctgaggat
     9001 gttccacatc gacgatcatc tgccggtgga gaacctgcgg gtcccgcaaa gtctccccca
     9061 tgtctttcac cggccccagg caaacatcct gcccctggaa taaggccacc cattcatccc
     9121 ggtctttctg gcggaaggtc ttccggagaa aggcataaat ctcctgacgc ttctcccctt
     9181 ccgtccactg atggggaatg aagtcctccc tccccaaagc ccggcaaaga ttggcccaga
     9241 aatgaggctc cagggggccc agggaaatgg actttccatc cttggtttca tagagagaat
     9301 agcaggggat gttcccagcg ccccaaaatt ctcctctctt gggagacctt ccggtggaga
     9361 gaagctccgc cagggcggca tgctgcaatc ccaccactcc gtcgaacatg gaaatatcca
     9421 cccattgccc ttttccctct ctctgggcag ccatgagggc caccagaatc cccactgcgg
     9481 ccatcaggcc ccctccgctg taatcggcaa caaaaaccga aggcagggcc gccggccgat
     9541 ctgcatatcc ttgcgggtcc aggatcccgc tgtagcccat atagttgagg tcatgacctg
     9601 ccagcaggcg atagggacca tcctgtccgt atccggagat ggaacaataa acgatccggg
     9661 agttaatttc tttcatccgc cgataatcga tccccaaccg ctccacgacc cccgggcgaa
     9721 atccttccac gatcacctgg gaggtgcgcg ccagagcgaa gaaaatctcc ttccccttgg
     9781 gggatttcag attcagggtt atgcttttct tgttccggtt gaggtccaga aaacgaattc
     9841 cccatccctt gatggaaggg ttttcggtcc ggaaccagtc tcctcccact tccgggtttt
     9901 ccactttaat cacctcggcc ccaaaatcgg ccaggaggtg ggtgcaatag ggccccggca
     9961 gagtgagggt caaatccaga atgcgaaccc cttttaatgc cgccatcgga tgcctccgtt
    10021 catttccttt ccccatgaaa cgaccagcgc tttaccgccg ggagcggagc gagcaaatac
    10081 cccccggttc ttcgggctga caaaggcctg ccaggattcc agatcccgtc tggatcaggg
    10141 aatccccgag atttccttgg ccaggtcctt tttctcctcg aagtcaacca accggttgag
    10201 gctgctcttc tgggccgccg gcggccccgc cccatgcatt gattccacca catagcccac
    10261 ggaattgatc ccgaagagga tgttctggat caggtaaatg agccgcatct tatgctccgt
    10321 gggaacctcg ggcaaccctc tcatgtattt ttccacatac ttgccgatct ccgggctttt
    10381 caaatctttc tcggacacca tggtgcccag gatccctccc gtgacgtcca ccgccagccg
    10441 ggccatctca aagggcaggg tgccaacatt cagcttgcac acattgccca gaagttgatt
    10501 gacaaagtaa gaacccgctt ctgtctcgcc tccttcaacc cccgagg
//
LOCUS       DASPRR010000551         8956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_944350, whole genome shotgun sequence.
ACCESSION   DASPRR010000551 DASPRR010000000
VERSION     DASPRR010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944350"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1458
                     /locus_tag="VEL68_20330"
     CDS             328..1458
                     /locus_tag="VEL68_20330"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXZ38386.1"
                     /translation="MHRLVCTRCARAFSPGEKLWNCTCGGLLNLEFDPAFPIEKIKKR
                     KPTLWRYREAIPIAKDDSILSFDEGFTPLVPLIFENKRVWVKQDHLFPTGSYKDRGAS
                     VLMSKVKELGISRVVEDSSGNAGCAIAAYGARGGIRSEIYVPENTSPGKLAQIESYGA
                     ILKKIPGSREDTARAVWQAAQKDYYASHSWNVYFFHGTKTFAFEVWEQMEWKAPDTLI
                     LPVGNGTLLLGAFIGFNELLEAGMIREIPRLVGVQSAHCAPLAKAFREGLKDIPKVQP
                     QDTLAEGIAIAEPVRGKQTLEAVKKSGGSFLAVNDGEVKRSLRLISQKGFYIEPTAAA
                     TLAGVLQYLRNSDPEEIIVSVFTGHGLKATEKILKILGEAAF"
     gene            1622..3238
                     /locus_tag="VEL68_20335"
     CDS             1622..3238
                     /locus_tag="VEL68_20335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXZ38387.1"
                     /translation="MGRPLADVGFLKALLTFGRYDTYYFFCPDLHHLKNFRKRLPELI
                     PDAKLQERVQTTLQVALAEILREQEFEVFHQGDFTYYMPQLIGLRNRLARVPFPVTGV
                     THSIDPIFMNSRYLELILAGPAPYDGIICSSPGARTAVEKGLGWVGKQLRERTGVTFP
                     TPLRLELIPLGIEESLFQEENRREARAYFHFPPDAIVVLSVGRISLRHKADWAPPLEL
                     FARMESQRELGKIILLIAGGGQSSDVTLLESMVNQMGLKKRILLFPNFAPEIKQKLYQ
                     ASDFYLSLIDNFQETFGLTVLEAMASGLPVVISDFSGYRDFISHGREGFLIPTTWAES
                     MPEFLKENLGILDPSMARLYLSQMVAVDLEKLKAAILRLARDARLRFRMGGAAKSKAL
                     TYRWSNLIPAYEKFWGELKEESRHYALNPTEPPNILAGDFSSTFSHYPSRRLSGKQEI
                     RLTDYGSQILKDSANPVRYEDVQVCLFPDLERFLLDSLLLQPRSVADLRELSLKSLDA
                     SKGQVDFILLWLLKHGAVKIESPWLNLEEG"
     gene            complement(3301..4386)
                     /locus_tag="VEL68_20340"
     CDS             complement(3301..4386)
                     /locus_tag="VEL68_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38388.1"
                     /translation="MERQWRFLKMGETYADCSISTSPALERGVMEGISPNTVLLDIFP
                     ADSFTIGVLDDPEKAIDLDFCRRRGIVVLRRNTTGGTIYAGKGSAMICYYLRLSEFGV
                     PKTIGDAFPKLLGDFARGARDLFGIPAQYRPLNDVEVEGRKLMPTSCKIEQDAMVLRV
                     VLNVKPQNVEITSQAILLPPEKVQDKALKTMEARFTYLEREAGREISPQDLETYIRRV
                     VRLSFGDVILTAGEMTDPERKYCREFSEKFSQETWFLANSESLRFKTIPPGVRRGEKR
                     VKAPAGLIRAVVLKKEGQIHDLILTGDFHPRPTSILADMEASLRGAPARAEEVSRRIQ
                     AFYGLPGVEISGTTPEDFISAVIGALE"
     gene            complement(4602..5246)
                     /locus_tag="VEL68_20345"
     CDS             complement(4602..5246)
                     /locus_tag="VEL68_20345"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXZ38389.1"
                     /translation="MAKIRILGQRFLFFTNLGLWIALFFVLTIAFTPLTQFMLRPLTI
                     QEEIKDADLVVVLGGGVDQGRYLTLISSHRMVRGIQLYFEGRAQKILFSGGIVGRATV
                     SEASVMNQEARRLKVPPEDILLEKKSKNTHDQAIEVKKIAEPLQMKSILLVTSFCSMK
                     RALLAFEQLGFKVYPAFADPHEKYTDEPLRRLSLFPKLVHEYGGILYYKLRGWI"
     gene            5584..6375
                     /locus_tag="VEL68_20350"
     CDS             5584..6375
                     /locus_tag="VEL68_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012274988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ38390.1"
                     /translation="MPVIFQKKEKIAIITINRPEAMNAIDPETSEELGKAWLEFRDNP
                     DLWVAILTGAGDKAFSAGADLKKMIPFLASLGPFDRKEREDKFPGFGGITRNLNIWKP
                     VIAAINGFCLAGGLEMALSCDLRVAAEHATFGLLEVSRGIIPGAGGTQRLPRMIPVAK
                     AMEIILMAQRIDAQEALRLGLVNRVVPAAEVMPTALKMAEQIQQNGPLAVRAAKEAII
                     RGMSLPLEEGLRLESVLQSYLLRTEDAVEGPKAFAEKRKPNFKAK"
     gene            6513..7154
                     /locus_tag="VEL68_20355"
     CDS             6513..7154
                     /locus_tag="VEL68_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HXZ38391.1"
                     /translation="MLEPQEFYQALRGVEVAPIEGLVRKALADGHSAAEVLNQGLIGG
                     MSIIGKEFKARDLWVPDVLLAARNMKQGVEILRPLFSREGQKSKGRIILGTVKGDIHD
                     IGKNLVAIMMSGSGFEVIDLGIDIPVEKFLSAIEEHRPQVVGLSALLTTTMLEMQKVI
                     EAVRKSPTLDKPFVIIGGAPVTEAYAREIGADGYGADAVAAVEVVLRLLEGGE"
     gene            7218..8243
                     /locus_tag="VEL68_20360"
     CDS             7218..8243
                     /locus_tag="VEL68_20360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HXZ38392.1"
                     /translation="MAQSLSPKRRFFSALFGGRKGDRPPVANPTSIVTLELMEKTGIF
                     FPQAHLDPKQMAELAAAGHEILGFDAIMPEFSVQQEAAALGCEMDWGSPSMMPDAKTH
                     PFQKVEDLKIPDHILEKPSMKIVLEALGILRRQYGDEVAIVGKVMGPWTISYHMAGTE
                     NFLLWSAIEPDKVRRFLEALKEVTVLFAKAQFQAGADVVVLADHATGDLVSPKTYQQF
                     LLPVHKELVQRIGGPLILHICGNCADRLRLFVEAGFDGYHFEWQVDAREAVKAVNHEM
                     ALVGNINNPTALYRGTPEDVRKQVRYSVEAGVDIIAPECAVPLQTPLRNLRAIVETAQ
                     EGYAEIN"
     gene            8489..8956
                     /locus_tag="VEL68_20365"
     CDS             8489..8956
                     /locus_tag="VEL68_20365"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HXZ38393.1"
                     /translation="MPDISIKFFMPMKVASVEKKGPREEMGKVLEQISQVLKEKKVKI
                     AGTPMALLDEGVKGIDLQKAHFEVCLPISGKVKGEGEVKSKELEKGAFACITHSGPSE
                     KLTEAYQELLKWIEENGYRIVGPSREVHHKGMGEGGGQPQDCMVELQFPVRKG"
BASE COUNT         2060 a         2472 c         2449 g         1975 t
ORIGIN      
        1 ttcctatttt tttccccgga ctcttgcaga tgagccggct gtcctaatgg attggcagga
       61 agcgatccag tccacccagg cgaagcgatc ttcagataca tctcgggatc gttacccgtc
      121 aaatgtacaa tttggtctgg aaatgaaatg ggaatggcta ggacacgaat cctcttgaac
      181 tcccggggat gatttaataa aatgtagagc tgtaaagccc atggggaagc tgtttggggc
      241 cttgggcaga cctgcgggcc tcggccacgg tattgggaat tctcgccttc cggctgagta
      301 gaaaaccagg ttcaagaggt gaatgctttg caccgactgg tttgtacccg atgtgcgcga
      361 gcgttctccc cgggggagaa attgtggaac tgcacctgtg ggggcctgct gaaccttgag
      421 ttcgatcctg ctttccccat cgagaagatc aaaaaaagga aacccacctt atggcggtac
      481 cgggaagcca ttcccatcgc gaaggatgac tccatccttt cttttgatga gggctttacc
      541 cccctcgtgc ccctaatctt tgaaaataaa agggtatggg tgaaacaaga tcatcttttt
      601 cccaccggtt cttacaaaga ccggggcgcc tccgttctca tgagcaaagt gaaggaactg
      661 ggaatctcca gggtggtgga ggattcctca gggaacgccg gttgtgccat tgccgcttac
      721 ggtgccagag gggggattcg gagtgaaatc tatgttccgg agaatacttc ccccggcaaa
      781 ctggcccaaa tcgaatccta tggtgcgata ttgaagaaga tcccgggctc ccgggaagat
      841 acggcccggg ccgtttggca agcggcccag aaggattact atgccagcca ctcctggaac
      901 gtctacttct tccatggaac gaaaaccttt gcctttgaag tgtgggaaca gatggaatgg
      961 aaagccccgg acaccctcat cctcccagtg ggaaacggaa cccttctact gggggctttc
     1021 atcggattta acgaattgtt agaggccgga atgatccggg aaatccccag gctcgtcggg
     1081 gttcaatcgg cgcactgtgc ccctctggct aaggccttcc gggaagggtt aaaggatatt
     1141 cccaaggtcc agcctcagga taccctggcc gaagggattg ccattgcgga acccgtgcgg
     1201 ggaaaacaga ccttggaagc agtgaaaaag agcgggggct ctttccttgc ggtaaacgat
     1261 ggggaggtga aaaggtccct gcggctcatc agccagaagg gtttttacat cgagccaacc
     1321 gcagccgcca ctctggcagg ggttctccag tacctgcgga attccgatcc ggaggagatc
     1381 atcgtctctg tcttcaccgg ccatgggctg aaggcgacgg aaaaaatatt gaaaatcctg
     1441 ggagaagccg ccttttaatc ccttttccga aagacccccg ggggatgctt ccttcttcct
     1501 cgccgccagc gctgggaaca ggctgggggg agaagaagac gcctgagcat aggaagaagg
     1561 tccgaggatg cgttttggca gcgttgatct tttcatggat ttcggggact ggaagttccg
     1621 aatgggacgc cccttggccg atgtgggttt tctgaaagcc ttgctgacct tcggccgata
     1681 tgatacttat tattttttct gccctgatct gcaccatttg aagaatttcc ggaaacgcct
     1741 gccagaactc attcctgacg ccaaactcca ggaacgggtt caaaccaccc tccaggtggc
     1801 cctggccgaa atccttcgcg aacaggagtt cgaggtattc caccaggggg actttaccta
     1861 ctacatgccc cagcttatcg ggctacgaaa ccggctggcc cgggttcctt ttcccgttac
     1921 cggagtgact cattccatcg atcccatctt catgaactcg cgctacctgg aattgattct
     1981 ggccggtccg gccccatacg acgggattat ttgctccagc cctggggccc ggacggctgt
     2041 cgagaaaggg ctcggctggg tgggcaaaca gctgcgggag aggacggggg tgaccttccc
     2101 caccccctta aggctggagt tgattccctt ggggattgaa gagtccctct tccaggagga
     2161 gaaccggagg gaagcccgag cttacttcca ctttcccccc gatgccatcg tggtcctttc
     2221 cgtcggccgc atttccctcc gccacaaggc tgactgggca ccgccccttg aactcttcgc
     2281 ccgaatggaa tctcagcgag agctggggaa aatcatcctc cttatcgccg ggggaggcca
     2341 gtcctcggac gtgaccctct tagagtcgat ggtaaaccaa atgggattga agaagagaat
     2401 acttctcttc cctaattttg ccccggaaat caagcaaaag ctgtatcagg cttccgactt
     2461 ttacctctcc ctcatcgaca atttccagga aaccttcggc cttaccgtct tggaggccat
     2521 ggcctcgggg cttccggtgg tgatttccga tttcagcggc tatcgggatt ttatatccca
     2581 tggccgggag ggttttctca ttcccaccac ctgggcggaa tcgatgcccg agtttctcaa
     2641 agagaattta ggaattctgg acccgtccat ggcccgcctc tatctttccc agatggtagc
     2701 cgtcgatctg gaaaagttga aggcggccat ccttcggctg gcaagggatg cgaggctccg
     2761 attcaggatg ggaggagcgg caaaatcgaa agccttgact tatcgctggt cgaatctcat
     2821 tcccgcctac gaaaaatttt ggggagagct gaaagaggaa tcccgccatt acgcccttaa
     2881 tcccaccgaa ccccccaata ttctggcggg ggacttctct tcaacctttt cccattatcc
     2941 ttcccggcgc ttatccggga agcaagagat ccgcttgacc gactacggca gccaaatcct
     3001 gaaagactcc gccaacccgg tccgttacga ggatgtgcaa gtctgcctct tccccgacct
     3061 ggagagattc ctcctggata gcctcctcct gcagccgcgg tccgtggccg acctgcggga
     3121 gctgagtcta aagtctctcg acgccagcaa ggggcaggtc gattttatcc tcctctggct
     3181 cctgaaacac ggggccgtga aaattgaatc tccctggctg aatttagaag aaggctaagc
     3241 tgcctccgcg ggttagaagc agtccgttct ggatgaagga tgcaccgccc gacccccctg
     3301 ctattccaag gcgccgatca ccgccgagat aaaatcttcg ggggttgtgc ccgagatttc
     3361 gactcccggc aggccataga aagcctggat tctccgggag acctcctctg cccgggccgg
     3421 cgccccccgg agagaggctt ccatgtccgc gagaatggag gtggggcggg gatgaaaatc
     3481 tccggtcaga atgaggtcgt ggatctgccc ttccttcttt agcaccacag cgcggatgag
     3541 cccggctggg gctttgactc tcttctctcc cctccggact cccggaggga tcgtcttaaa
     3601 tctcagcgat tcggaattcg ccaaaaacca ggtttcttgc gaaaactttt cgctgaattc
     3661 ccggcagtac ttcctttcgg ggtcggtcat ttctcccgcg gtcagaatca cgtccccgaa
     3721 ggacagccga acgactctgc ggatatatgt ctccaggtcc tgcggtgata tttcccgccc
     3781 tgcttctcgc tccaggtacg taaaccgcgc ctccatggtc ttcaaagctt tgtcctggac
     3841 tttctccggg ggcaagagga tggcctggga ggtaatttcc acattttgcg gtttcacatt
     3901 gagaaccacc cgcaagacca ttgcgtcctg ctcgatcttg caggaggtgg gcatgagttt
     3961 ccgcccttcc acctccacat cgttcaaagg gcggtattgg gccggaattc caaagaggtc
     4021 ccgggctccc cgggcaaagt cccccagaag cttggggaag gcatccccga tcgtcttcgg
     4081 caccccgaat tcggacaatc gcaggtagta gcagatcatc gccgatccct tgccggcgta
     4141 aatcgttccc ccggtggtgt tccgccgcag gacgacgatc cctcgccggc ggcagaaatc
     4201 caagtcgatt gccttctccg ggtcatccag gactccgatg gtaaaactgt ccgctggaaa
     4261 tatatccaga agaaccgtat tgggagagat tccctccatg accccccgct ccaatgcagg
     4321 agaggtgctg atcgaacaat ccgcataggt ctctcccatc ttcaagaatc gccactgccg
     4381 ttccatattc ctcctcccaa agaaaggtaa gaggtgagag gttggacgtt agaggctgaa
     4441 agatggagga gtaggattca tcacccaatc cgtgcgcctc cggcctctga cttcaaacca
     4501 attttccccc tcttcgttct tttttgcctc caatcttaaa cctccaacca aatctccttg
     4561 tctttgcctc caacctcaaa cctccgacct ccaacctttt cttatatcca tcctctaagc
     4621 ttgtagtaaa ggatccctcc atattcatga accagcttgg ggaaaaggct cagacggcga
     4681 agaggttcat cggtgtactt ttcatgggga tcggcaaagg ccggatagac tttgaacccg
     4741 agttgttcaa aggccaacag cgctcgtttc atactgcaga aggaggtaac cagaaggatg
     4801 ctcttcatct ggagaggctc cgcgattttc tttacctcaa tggcctggtc atgggtgttt
     4861 ttcgatttct tctccagaag gatatcctcc ggcgggacct tcaaccgtct tgcctcctga
     4921 ttcataactg atgcctcgga gacggttgcc cggcccacga ttcccccgga gaaaaggatc
     4981 ttttgggctc taccctcgaa atagagttgg atgccccgga ccattcgatg ggaagatatt
     5041 agggtaagat agcggccctg atccacgcct cccccgagga ccaccacgag gtccgcgtcc
     5101 ttaatctcct cctgaatggt tagtggtctc agcatgaatt gagtcaaggg ggtaaaggcg
     5161 atcgtcagga cgaaaaatag ggcaatccac aggccgagat tggtgaagaa taaaaacctt
     5221 tgccccagta tgcggatttt cgccatcttc ttctcccaag cgctttttcc ctggggtcat
     5281 tatatgagag gggatttctt aaaggcaaga aaaacaagag aatcgttccg agttgctggt
     5341 tgctccttgc tggttgctgg tttcaggttt gtagatcttt ggatactgac tcctgccttc
     5401 tcatctcgta gcttaaattc atccccaacc ccccatccgg aaacgaacga gcaccgagta
     5461 tccaacagcg agcacccagc aactgggaat aacaaccagc atcaaattct tttcttgaca
     5521 gggtaaaccc attcccttat aaaaagcata gatttccgat gaacctggaa aaggaggaaa
     5581 accatgccgg ttatttttca aaagaaagaa aagatcgcca tcatcaccat caaccgcccg
     5641 gaggcgatga acgccatcga ccctgagacc agtgaagagc tgggcaaggc ctggctggag
     5701 ttccgcgaca atccggacct ctgggtggcc atcctcaccg gagctggcga caaggctttt
     5761 tccgccggcg ccgacctgaa aaagatgatc cctttcctgg cttccctggg ccccttcgat
     5821 cgaaaggagc gggaggacaa attcccaggg ttcggcggca ttacccggaa cctcaacatc
     5881 tggaagcccg taatcgccgc aatcaatggc ttctgcctgg cgggtggcct ggagatggcc
     5941 ctgtcctgtg acctccgtgt ggccgccgaa catgccacct tcggcctgct ggaagtgagc
     6001 cggggcatca tccccggagc tgggggaacc cagcggttgc ctcggatgat tccggtggcc
     6061 aaggccatgg aaatcatctt gatggcccaa cgcatcgacg cccaggaagc cttgcggttg
     6121 gggctggtca accgggtagt gcccgcggcc gaggtcatgc ccacggccct gaagatggct
     6181 gagcagatcc agcaaaatgg acctttggcg gtgagggccg ccaaagaggc catcatccgg
     6241 ggaatgtccc ttcccctgga agaagggctc cgactggaat cggtcctcca atcctatctc
     6301 ctccggaccg aagacgccgt ggaaggcccc aaggcctttg ccgagaagag aaagccgaat
     6361 tttaaggcga agtgaagaca gagaaagtga aagtgcaagg gtaagggcaa ggacgaaacg
     6421 ggcataaaat tgcgaaggcc tgtactttca cttccactta cactttcact tgcccttaca
     6481 ctttccttca tagccgttgc ggagaagaca tcatgctcga gccccaggaa ttctatcagg
     6541 ccctacgagg ggtggaagtt gcaccgattg aaggtctggt cagaaaagcg ctggctgacg
     6601 gccattcagc cgcagaggtc ctcaatcaag gattgatcgg cgggatgtcg atcatcggga
     6661 aggaattcaa agcccgggac ctctgggttc cggatgtcct cctggccgcc cgcaacatga
     6721 agcagggtgt cgaaatcctt cgcccccttt tctcccggga aggccaaaag tccaagggaa
     6781 gaatcattct agggacggta aagggagaca ttcacgatat cggcaaaaac ctggtggcca
     6841 tcatgatgtc ggggtccggc ttcgaggtca tcgacttagg gatcgatatt cctgtagaaa
     6901 aattcctttc cgccatcgag gagcacaggc cgcaggttgt cgggctttcc gccctcctta
     6961 ctactaccat gctggaaatg cagaaagtca tcgaagctgt aagaaaaagc ccaaccctcg
     7021 acaagccttt cgtcatcatc gggggcgccc cggtgaccga ggcctatgcc cgggagatcg
     7081 gcgcggacgg gtacggggcg gatgcggtgg cggcggtgga agtggtgttg agactattag
     7141 aagggggaga gtaggcgaag gggcgaagag gggaaaaggc gaaaagggaa aaatcataag
     7201 gaaagaggag aatccagatg gctcagagtc tatcaccaaa gcgacggttc ttttctgctc
     7261 tcttcggggg gcggaaagga gatcgtcccc ctgtcgccaa tccaacttct atcgtcaccc
     7321 tggagttgat ggaaaagacg ggaattttct ttccccaggc ccacctggac ccaaaacaga
     7381 tggccgagct ggcggcggcg ggccacgaga ttcttgggtt tgatgccatt atgcccgaat
     7441 tcagtgtcca gcaggaggcc gccgccctgg gttgcgaaat ggactgggga agcccttcca
     7501 tgatgcccga cgccaagacc caccccttcc aaaaagttga agacctgaaa atccccgatc
     7561 atatcctgga aaagccttcg atgaagatcg tcctggaagc cctgggcatc ctgcgccgcc
     7621 aatacgggga tgaagtggcc atcgtcggga aagtcatggg gccctggacg atatcctacc
     7681 acatggccgg gacggaaaat tttctccttt ggtcggccat agagcctgac aaagtgaggc
     7741 gctttttgga agcgctgaaa gaggtcactg tcctctttgc caaagctcaa ttccaagccg
     7801 gggccgatgt agtcgtcctg gctgatcatg ctaccgggga tctggttagc cccaaaacct
     7861 accagcagtt tctccttccg gtgcacaaag aactcgtcca gcgcatcggc ggccctttga
     7921 ttctccatat ctgcgggaac tgcgcggacc ggctgcggtt gttcgtagaa gccggttttg
     7981 acggctatca ttttgaatgg caggtggacg cgcgggaagc ggtcaaagcg gtgaatcacg
     8041 agatggccct ggtaggcaat attaacaatc ccacggccct ttaccgcgga acgcccgagg
     8101 atgtccggaa acaggtgcgc tatagtgttg aagcgggcgt ggacatcatc gctcccgaat
     8161 gcgccgtccc cctccagacc cctttgcgga acctccgggc cattgtggaa accgcccagg
     8221 aagggtatgc agaaatcaat tgacacgatt tcgctttctt tgtagaatac gcccggaatc
     8281 gcgcgaaccc ttgtcgcttc tcccttaatt agaaacctag aactcccaag aatggcctga
     8341 cctttttctt tcctcactgc ggaaagaaaa agcctccttc tgtgattggc agggaaccct
     8401 tctgaattag gcttttgggc ctaccatatt caggatttgt tcactccccc ccggggagtg
     8461 tattttaggg tgaaaggagg tcattctcat gccggatatt tccataaaat tcttcatgcc
     8521 catgaaggtg gcgtccgtgg agaaaaaagg gccccgcgag gaaatgggta aagtccttga
     8581 gcaaatctcc caagtcttga aagaaaaaaa ggttaagatt gctggaacgc ctatggcctt
     8641 gctcgatgag ggcgtcaaag ggattgatct ccagaaggcc catttcgaag tctgcctgcc
     8701 catttcagga aaagtcaaag gggaagggga ggtcaaaagc aaagagctgg aaaaaggggc
     8761 ctttgcctgc atcacccatt ccggcccctc ggagaaattg acggaagcct atcaggagct
     8821 cctaaaatgg atcgaagaaa atggctaccg catcgtcggc cctagtcgcg aagtacacca
     8881 taaaggaatg ggcgaaggtg gggggcaacc ccaagattgc atggtggagc ttcaatttcc
     8941 cgtgaggaaa ggatag
//
LOCUS       DASPRR010000552         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_616856, whole genome shotgun sequence.
ACCESSION   DASPRR010000552 DASPRR010000000
VERSION     DASPRR010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616856"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VEL68_20370"
     CDS             complement(<1..175)
                     /locus_tag="VEL68_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ38394.1"
                     /translation="MLQVKGISVFYGDVQALREVSLSVSTGEIVSLVGGNGAGKTTAL
                     KTISGLLRPASGSI"
     gene            complement(162..905)
                     /locus_tag="VEL68_20375"
     CDS             complement(162..905)
                     /locus_tag="VEL68_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38395.1"
                     /translation="MVLLECTGVTKYFGGLAALHQVDLQVSAGEIVGLIGPNGSGKTT
                     LFNVITGLYPLSKGSIRFKGEEIGGLRPFRVCQKGIGRTFQTSKPFTRMTVLENVMVG
                     ALYGKGAAGEDLEKARTSSEVILEQAGLRAKKNARISDLTLEDKKRLELSKALATRPE
                     LLLLDEVMAGLNPTEIKEIMGLIGKIRQEGMTIIVVEHVMHAVMNLADRVCVLHHGEK
                     IADAPPEEVSRDKRVIESYLGEEFLLASS"
     gene            complement(968..1930)
                     /locus_tag="VEL68_20380"
     CDS             complement(968..1930)
                     /locus_tag="VEL68_20380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38396.1"
                     /translation="MLNRGYIQFLALILLLGSLALLPLFLSQYSLHLMILVMVFVVIS
                     TSWNILVWTHQISLGHAAFFGIGAYTAAVVYNAWQLPPYFDMLLGGVLSSVGAVIIGV
                     ICLRMAPWALAIATLAFAEALKILVIMLPGLTEGAEGVALPPLYGGGESAKVYAYYLV
                     SGIAFLTVLTAFLLKRSRLYFAFTAVHDDQDAASMLGINPTKYKVMAFTISAFFVGIV
                     GSFYAHYISYIDPHAAFNIHISVEAQIMPLMGGLYTVAGPVVGAFILTLLGEYLRITV
                     KTGYLIIYGIILVLLIFYLPKGLVGLMENWTRKEGRALSSEKLR"
     gene            complement(1920..>2186)
                     /locus_tag="VEL68_20385"
     CDS             complement(1920..>2186)
                     /locus_tag="VEL68_20385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38397.1"
                     /translation="GAAGMVTSMNMYIFPLVGMLFILKAFAIVVLGGFGSIAGAVVGS
                     LILGLTESLVTNYLPNGSGWAEGVSFLILIVILIIRPKGLFYVE"
BASE COUNT          522 a          628 c          627 g          409 t
ORIGIN      
        1 gaatggaccc ggaagccggc cggagcagcc cggagatggt tttgagcgca gtggtcttgc
       61 cggcgccgtt tcctcccacc agggagacga tttctccagt ggaaaccgag agggaaactt
      121 cccgcagggc ctggacgtcg ccgtaaaaga cactgatccc tttaacttga agcaaggaga
      181 aactcctccc ccaggtagga ttcgataacc cgcttgtccc tggagacctc ctccggcggg
      241 gcgtcggcga tcttctcccc atgatggaga acgcagacgc ggtcggccag gttcatgacc
      301 gcgtgcatga catgttccac cacgatgatc gtcatccctt cctgccggat tttaccgatg
      361 agccccatga tctctttgat ctccgtgggg ttgagccctg ccatcacttc gtcgaggaga
      421 agaagttcgg gccgggtggc cagggctttg cttaactcca ggcgtttctt gtcctccagg
      481 gtcaggtcgg agattcgagc gttctttttg gccctgaggc ctgcctgttc cagaatcacc
      541 tccgacgagg tcctggcttt ttcaaggtct tccccggccg ctcctttccc ataaagagcg
      601 cccaccatca cattttccag gacggtcatg cgggtaaaag gcttggaggt ctggaaagtc
      661 cggccgatcc ccttttggca aacccggaag ggccggaggc cgccgatctc ctcccccttg
      721 aagcggatag accccttgga gagaggatag aggccggtga tcacattaaa gagcgtcgtc
      781 ttcccggagc cattgggtcc gatgaggccg acgatctccc ctgcggaaac ctggaggtcg
      841 acctggtgca aagccgccag gccgccgaag tatttcgtaa cccctgtgca ttccaaaaga
      901 accatagccc ttcgccaatg cggaatttgg attgcggatt gcggaatgaa aattcaaaaa
      961 cccgaaatta acgcaatttc tccgatgaca gcgcccttcc ttcctttcga gtccaattct
     1021 ccatcagccc cactaaccct ttgggcaggt agaagatgag cagaaccagg atgatcccgt
     1081 aaatgatcaa gtacccggtt ttgaccgtaa tccgaagata ctctcccagg agagtgagaa
     1141 tgaaggcccc caccaccggg ccggccacgg tgtagaggcc tcccatcagg ggcataatct
     1201 gggcttctac cgagatgtgg atgttgaaag cagcatgggg gtcgatgtac gagatgtaat
     1261 gggcgtaaaa gcttcccacg atccccacaa agaaagcgct gatggtaaag gccatgactt
     1321 tatactttgt ggggttgatc cccagcatgc tggcggcgtc ctggtcgtcg tggaccgcgg
     1381 taaaagcgaa atagagcctg gaccgcttca gcaggaaagc ggtgaggacg gtcagaaaag
     1441 caatcccgga taccaggtag taagcgtata ccttggcgct ttctcccccg ccgtaaagag
     1501 gaggaagagc caccccctcg gctccctcgg taagccccgg gagcatgatc accaagattt
     1561 tcagggcctc ggcaaaggcc agggtggcga tggccagggc ccagggagcc atgcgcaggc
     1621 agatgactcc gatgatcacc gcccccacgg aggaaaggac ccctccgagg agcatatcga
     1681 aataaggggg aagctgccag gcattgtaga ccaccgcggc cgtgtaggcg ccgatcccga
     1741 aaaaagccgc atgccccagg gaaatctgat gggtccagac caggatgttc cagcttgtgg
     1801 agatgaccac aaagaccatc accaggatca tgagatgtag agagtactgg ctaaggaaaa
     1861 ggggcagcag ggccagcgac cccaggagaa ggatcagggc aaggaactgg atgtaccctc
     1921 tattcaacat agaagagccc cttagggcgg atgatcagaa tgacgatgag gatgaggaaa
     1981 gacacccctt cggcccaccc ggacccgttg gggagatagt tggtaaccag agactcggtc
     2041 agccccagga tcagacttcc aaccaccgct ccggcaatgg acccgaaccc ccccaggacc
     2101 acgatggcaa aagccttcag gatgaagagc atgcccacca gagggaaaat gtacatgttc
     2161 atgctggtaa ccatgcccgc ggcccc
//
LOCUS       DASPRR010000553         6153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_451309, whole genome shotgun sequence.
ACCESSION   DASPRR010000553 DASPRR010000000
VERSION     DASPRR010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451309"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VEL68_20390"
     CDS             complement(<1..858)
                     /locus_tag="VEL68_20390"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXZ38398.1"
                     /translation="MNVIAGVDIGGTAIKIGLVSDEGTLQAKASIDYDPQKTFDRLVN
                     SVCDSLRLLRSRTNADLKAIGISTPGYADPNTGILIDGANNVPALQGQSLPGALGSRF
                     NLPAFIDNDGTCAATGELHFGAGRSFKNFALITLGTGIGGGVVINRKPVTGRDGQPPE
                     IGAICLDPDGPVNYSGVPGSFERLACASGFVDLYRKFGGREEAGMTPQEIFERAAQGD
                     RAAESAIDQEGKYIAQAFGVMINLLNLEACIIGGGISAAGPPLVDAVRKHLPSFTWPN
                     LFRKSQVLLA"
     gene            complement(855..1898)
                     /locus_tag="VEL68_20395"
     CDS             complement(855..1898)
                     /locus_tag="VEL68_20395"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXZ38399.1"
                     /translation="MDILSYIYEQPQVVERVLKEVPRDLAAIPKVSPAREIFLVGSGT
                     SMNTLVAVEPLLASSVNSRVRIQGPLSFLSEPGREHDQDSLVVILSQSGTSTTTIQAV
                     ENARRLGMRTITVTAERESPIARISSEILKIPAGPEPVGPKTKGYTGGVLTLLLMILV
                     QTGRVLEAPRFPAELSSLIDHCKIACKELARTFRGTDFVMVMGQGRHYGTALEGSLKI
                     TEMSGTPAAAFETEEAFHGRFHGLGRKSLALFVTASPDQQEMAASGAEVLSGLGVGVH
                     ILNLARGPSSPFDFHMPWPATNALPELDLLSAIVPFQFLAWHLAGEKGIVPEKMKYPG
                     LSQKLGIKTKVNL"
     gene            complement(1904..2746)
                     /locus_tag="VEL68_20400"
     CDS             complement(1904..2746)
                     /locus_tag="VEL68_20400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ38400.1"
                     /translation="MASKRARRKLTQGFIYSFLVIASAVVLFPYLWMALTSFKPIEEF
                     FTYPLQWFSKNWTLAQYAGVLEDPKFMACLWNSLLVSSIVTAISLVLSIPAAYGLTRL
                     RAPGGRSILVGILSSQFFPPMIFFVPFYIILSKFRLLNTIPGLVFAYLSVTLPICTWM
                     VATTFRNIPRELEEAAKVDGCGDIKTIFEVVLRMSLPGILTAGLFSFVLAWQEYIFAL
                     LYTTTTAAQTAPVLLFFYLGQHQIDYGRLMAAAILLSLPVIFPFAFMQRYFRQGRGEG
                     SIKG"
     gene            complement(2757..3629)
                     /locus_tag="VEL68_20405"
     CDS             complement(2757..3629)
                     /locus_tag="VEL68_20405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ38401.1"
                     /translation="MLKGRTKFIKGYLFAAPLLVVLAVAVLFPALYNLGIAFSDYNVT
                     TNSWRFAGMQNFAKLWNSPFFWNSFKVTLLWVAGNVSLQLFVGLAIALGLNSIIRFRG
                     PFTGILLIPWISSFVVVGILFIWIYDPQMGVLNDILLRLGLIEKPVAWLASPHLAQTS
                     LIAANTWKFFPLVMITLLAGLQDVSPELVEATQVDGANWLGTFRMVIFPTILPSISTG
                     VLLSTIWAYNSFTLPIVMTGGGPLRATEIFGLYIFKLAFDAFDFGAAAAASLILFTQI
                     LIVIVLYLKVVERD"
     gene            complement(3782..4816)
                     /locus_tag="VEL68_20410"
     CDS             complement(3782..4816)
                     /locus_tag="VEL68_20410"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ38402.1"
                     /translation="MDRHVRVAMLGTGFIAGFRTQVYQRLQGVKVASVVGRDPARTRK
                     FAEANHIGFAATSFEELLKGPDFDAVDLCLPNHLHLAAGIQTAKAGKHILCEKPLGRT
                     AEEAEAMLEAAEAARIIHCYGENLLFTPDMQEILSIVQRGTIGAPLWMRGREGHFGPH
                     SPWFYEHAFSGGGALLDMGCHVIGAFNFISKQKAKAVFAHTPTLHHSTDCEDNALAII
                     RYGEKLVGQAEASWTQRGGMAVVFEAWGDEGMITYDRSGLSQPIKVFSKKQTDTYFSE
                     KVEHKQGWLFPTVQEYWRYGYYDEISHFVECVREHKKPMLTFRDGLEVNRIIDRAYAS
                     GRSGKWEALD"
     gene            complement(5007..>6153)
                     /locus_tag="VEL68_20415"
     CDS             complement(5007..>6153)
                     /locus_tag="VEL68_20415"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXZ38403.1"
                     /translation="KIAYPGWDSKDQEKEVTSIFAAYEKQVPGMKVEIISIPFHMMKQ
                     KLVVSLRSGDAPDVGYVDGRWLQEMQAAGFLTDLTDYSNTLDKKDFFELPWKTATVEG
                     KIYAIPDRIDPWMFYYNTDMFKAAGIKEFPKTMDELVEVCKKITVAGKQYGYGMVGIN
                     DATFIGRFLNILYAFHGDFLTPDGKKAAINNEAGVAAFQFYGDLLNRYKVAQPSAIAN
                     SNADVRKLLLTSQVGMMIDGPWATGTLRQQNPNLNWYVGQIPRVKDMKPRFTMSSWYY
                     VIFSQSKVKDESKKFATYILRPENQARSVVTIPGRKSAAAMPRFQTRDWKPWVEAAPY
                     GEPLPITDKFSQIADIVGNASQQVLSQKATAKQAADQAAEQINNLLK"
BASE COUNT         1321 a         1752 c         1797 g         1283 t
ORIGIN      
        1 ggccaggagg acctgggatt tgcggaagag gttgggccag gtgaaggacg gcaagtgttt
       61 gcggacggca tccactaacg gtggaccggc ggcggaaatt cccccaccga taatgcaggc
      121 ttccaggttg aggaggttaa tcatgacccc aaaggcctga gcgatgtatt tcccttcctg
      181 gtcaatcgcc gattcggcgg cccgatctcc ctgagcggcc cgctcaaaga tttcctgggg
      241 ggtcatgccg gcctcttccc ttcctccgaa tttcctgtag agatccacga accccgaagc
      301 gcaggcaagg cgttcaaaag acccggggac tccgctgtag ttcaccgggc cgtccgggtc
      361 caggcagatg gccccgatct ccggcggctg cccgtcccgg ccggtcaccg gcttgcggtt
      421 gatcacgacg ccgccgccga tacccgtgcc aagggtaatt agggcaaagt ttttgaagct
      481 tcgcccggcg ccgaaatgaa gctcaccggt ggctgcgcag gtcccgtcgt tgtcaatgaa
      541 ggcgggtagg ttgaaccggc ttccaagcgc gccggggagc gattgtccct ggagggcagg
      601 aacattgttc gccccatcga taagaatgcc cgtgttgggg tcagcgtaac ccggggtgga
      661 aatcccaatg gccttcaaat cggcatttgt ccgggagcgg agcaatcgta gggaatcgca
      721 gaccgagttc accagccgat caaaggtctt ttgggggtca tagtctatgc tcgccttcgc
      781 ctgcaaggtc ccctcgtcgg agaccaatcc aatcttgatc gctgtcccgc cgatgtcgac
      841 tccggcaatt acattcatag gtttaccttt gtcttaatcc ccagcttctg cgaaagacct
      901 ggatatttca tcttttccgg gacgatccct ttttccccgg ccagatgcca ggccaggaat
      961 tggaaaggga caatggcgct taaaaggtcc agttcgggta gggcattggt tgccggccag
     1021 ggcatgtgga aatcgaaggg ggatgaaggt cccctcgcta ggttcaagat gtgcacccca
     1081 acccccagcc cggagaggac ctcggcgccg ctggcggcca tctcctgttg atccggcgaa
     1141 gcggttacaa acagggccag gcttttcctg cccaatccgt ggaagcggcc gtgaaaagcc
     1201 tcctcggttt cgaaggcggc tgccggtgtc ccgctcattt cggtgatttt cagagagccc
     1261 tcaagggccg tgccgtaatg ccttccttgc cccatgacca tgacgaaatc cgtcccgcga
     1321 aaggttctgg ctaactcttt gcaggctatt ttgcaatggt caattaagct ggaaagctcc
     1381 gccggaaagc ggggggcctc gagcacccgg ccggtctgca cgagaatcat taagaggagg
     1441 gtgagcactc cgcctgtata tcctttcgtc ttggggccca cgggttccgg cccggccggg
     1501 attttcagta tttccgaaga tatgcgggcg atcgggctct cccgctccgc agtcaccgtg
     1561 atcgtcctca tgcccaggcg gcgggcattt tcaaccgcct ggatcgtggt ggtgctagtt
     1621 ccgctttgag agaggatgac cactagagaa tcctggtcat gctcccttcc gggttcagac
     1681 agaaaagaca ggggcccctg gattcgaacc ctggaattca ccgatgaagc cagcagtggt
     1741 tctacagcca ccagggtatt catcgatgtg ccggaaccca caagaaaaat ctctcgggcc
     1801 ggcgagactt tgggaattgc cgcaaggtcc cggggaactt ctttaagaac ccgttctacg
     1861 acctgcggtt gttcataaat atacgagaga atatccattc cccttatcct ttgatgctcc
     1921 cttcccccct cccctgccgg aaatacctct gcatgaaagc gaaggggaaa atgactggga
     1981 ggctgagcaa tatggccgca gccatgagcc ggccgtagtc gatctggtgt tgtcccaggt
     2041 agaagaagag gaggaccggc gcggtctggg cggctgttgt ggtggtgtac aggagggcaa
     2101 atatgtactc ctgccaggcc aggacgaatg aaaagaggcc ggcggtaagg atcccgggca
     2161 aggacatacg cagcacgacc tcgaaaatcg tcttgatatc cccgcatccg tccactttcg
     2221 ccgcctcttc cagctcccgg gggatgtttc ggaaggtggt ggccaccatc caggtacaga
     2281 tgggcagggt gaccgaaagg tatgcgaaga cgagaccggg gatcgtattc agcagcctga
     2341 atttagaaag gatgatgtaa aagggaacga aaaagatcat gggcgggaag aactgggaac
     2401 tcaggattcc caccaaaatg gatcttcccc cgggggcccg caaccgggtc aggccgtaag
     2461 ccgcggggat ggagaggacc aggctgatgg cagtgacgat ggaagagacc aggaggctgt
     2521 tccacaggca ggccatgaac ttcgggtctt ccaggacccc cgcatactgg gcgagagtcc
     2581 agttcttgct aaaccactgg agggggtagg tgaaaaactc ctcgatgggt ttgaagctgg
     2641 tcagggccat ccaaagatac gggaaaagga ctaccgcgga ggcgatgacc aggaaagaat
     2701 agatgaaccc ctgggtcagt ttccttcgcg cgcgcttcga agccatgagg gacatatcaa
     2761 tctcgctcca ccaccttcag gtagaggacg atgacgatca gaatctgggt gaagagaatc
     2821 agcgaggcag ccgccgccgc gccgaaatca aaagcgtcaa aggccagctt gaaaatatac
     2881 agcccgaaga tctccgtggc ccgcagaggg cctcccccgg tcatgacgat cggcagggtg
     2941 aaggagttat aggcccagat ggtggatagc aggaccccgg tggagatact cgggagaatg
     3001 gttgggaaaa tgaccattcg gaaagtcccc agccagttgg ccccatccac ctgggtggcc
     3061 tctacaagtt ccggggatac gtcctgcagg ccggccagaa gagtgatcat gaccaggggg
     3121 aaaaatttcc aggtattggc cgcgatcagg gaggtctggg cgagatgcgg agaggccagc
     3181 caggcaacgg gcttttcgat caagccgagg cgcagtagga tgtcattcag cacccccatc
     3241 tggggatcgt aaatccagat gaaaaggatc cccaccacca cgaaagagga gatccagggg
     3301 atgaggagaa tcccggtaaa gggcccgcgg aaccggatga tggagttcag ccccagggcg
     3361 atggccaggc cgacaaacag ctgcagggaa acgttgcccg ccacccacag aagggtgacc
     3421 ttgaaggaat tccagaagaa gggggaattc catagcttgg caaaattctg catccccgcg
     3481 aaccgccagg aattggtcgt cacgttgtaa tcagagaaag caatccccag gttgtagaga
     3541 gcgggaaata gcactgccac agcgaggacc accagcagag gggcggcaaa gaggtagcct
     3601 ttgatgaatt tggtgcgacc ttttaacaat ccttcagcct gctcctacat ccgcatttga
     3661 ttcaccgcgg cgcgatcggg gatcgcgccc tacaaaatcc gcttggataa accataaggc
     3721 tcgcgaatct ccaggcatca aaaatgtagg gtgcgttccc cgaacgcacc ggtctcccgg
     3781 attaatccag agcttcccac ttccctgacc gcccggaggc gtaggccctg tctatgatcc
     3841 ggttcacttc caatccgtcc cggaaggtca acatcggttt cttgtgctct cgcacgcact
     3901 ccacaaaatg gctgatttca tcgtagtagc cgtacctcca atactcctgg accgtgggga
     3961 atagccaccc ctgtttgtgc tccacctttt cgctgaaata ggtgtcggtc tgcttcttgg
     4021 aaaagacttt gatgggttgc gagagtccgg aacgatcata ggtgatcatt ccctcgtccc
     4081 cccaggcctc aaaaaccacg gccattcccc cccgctgcgt ccagctggcc tcggcctggc
     4141 ccaccagctt ttccccatac cggatgattg ccagggcgtt gtcttcgcaa tctgtgctgt
     4201 ggtgaagggt cggggtgtga gcgaagaccg ctttcgcttt ctgcttgctt atgaaattga
     4261 aagccccgat cacatggcag cccatatcca gaagggcccc gcccccggaa aaggcatgct
     4321 cgtaaaacca gggggaatgg ggcccgaagt gaccttcccg cccccgcatc cacaagggag
     4381 caccgatcgt ccctcgctgg acgattgaga gaatctcctg catgtccgga gtgaagagaa
     4441 gattttctcc gtagcaatga atgatccggg ccgcctccgc cgcttccagc atggcctcgg
     4501 cttcctccgc ggtgcggccc aggggcttct cgcagagaat atgcttgcct gctttggccg
     4561 tttggattcc tgctgccagg tgaaggtggt tgggcaggca aaggtccacg gcatcgaaat
     4621 ccggcccctt caggagttcc tcaaaggagg tcgccgcaaa accaatgtgg ttagcctcgg
     4681 caaattttcg cgtgcgggcg ggatcccgtc ccacgacgga ggccactttc accccctgga
     4741 gtcgctggta cacctgggtg cggaatccgg ctatgaaccc ggtccccagc atggctaccc
     4801 gaacatgcct atccattcat ccgctccgga atatgggagg gtgtgcaaaa ttggatttca
     4861 ttcgccaccc cggcgaaaac cggggtccag tcagttcgta agaccctgaa aacactggat
     4921 tccggctttc gccggaatga cataaaaaaa ccaaatcgat tttttcgcac cttctggctc
     4981 cctaagagcg ggtttgaagc ccgcccctac tttagaaggt tgttgatctg ctctgccgcc
     5041 tgatcggccg cctgcttggc cgtggctttt tgcgaaagaa cctgctgcga ggcattgccg
     5101 acgatatccg caatctggct gaatttgtcg gtaatgggca ggggctcccc gtagggggcg
     5161 gcttccaccc acggtttcca atctctggtc tggaacctgg gcatggccgc ggcggatttc
     5221 cgcccgggga tcgtgaccac gcttcgggcc tgattctccg gccgaaggat gtaggtggcg
     5281 aatttcttgc tctcgtcctt gaccttgctc tgcgaaaaga tcacgtagta ccaggaactc
     5341 atggtaaacc gtggcttcat gtccttcacc cgcggaatct gccccacgta ccagttcagg
     5401 ttggggttct gctgacgcag ggtgccagtc gcccacgggc cgtcgatcat cattcccacc
     5461 tggctggtca ggaggagttt tcgcacgtcc gcgttgctgt tggcgatggc cgaaggctgg
     5521 gccaccttat atctattcaa caggtctccg tagaactgga aggccgccac tcccgcctca
     5581 ttgttgatag ccgccttttt cccgtccggg gtcaggaaat cgccgtggaa ggcgtacagg
     5641 atgttgagaa agcggccgat gaacgtggcg tcattgatac ctaccatgcc gtatccgtac
     5701 tgtttgccgg ccacagtgat ctttttgcac acctccacca gttcatccat ggtcttggga
     5761 aactccttga tcccggcggc cttgaacata tccgtattgt agtagaacat ccacgggtcg
     5821 atccggtcag ggatggcgta aatcttgcct tccaccgtgg ccgttttcca gggcagttcg
     5881 aagaagtctt tcttgtccag ggtgttggag tagtccgtca ggtcggtcag gaacccggcg
     5941 gcctgcatct cctgcagcca gcggccgtcc acgtaaccca cgtccggggc atcgccggag
     6001 cgcagagata caaccagttt ctgtttcatc atatggaagg ggatgctgat gatttccacc
     6061 ttcataccgg ggacttgttt ctcataggcc gcaaaaatgc tggtgacctc tttctcctgg
     6121 tctttgctgt cccatcccgg gtaggcaatc ttg
//
LOCUS       DASPRR010000554         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_451702, whole genome shotgun sequence.
ACCESSION   DASPRR010000554 DASPRR010000000
VERSION     DASPRR010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451702"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VEL68_20420"
     CDS             <1..363
                     /locus_tag="VEL68_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157212186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HXZ38404.1"
                     /translation="GQHVNKTESAVRITHIPTGIVVQCQNERSQHKNRAMAMKVLKSR
                     LYEMKLREQEEKAEALHSAKKDIAWGSQIRSYVLQPYRLVKDHRTNFEMGNVDAALDG
                     DLDEFIQAYLLQKAAKSA"
     gene            664..1416
                     /locus_tag="VEL68_20425"
     CDS             664..1416
                     /locus_tag="VEL68_20425"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ38405.1"
                     /translation="MELTVLGSGTCIPAVDRGPSGLALTFGSHLILFDGGGGSLRPMP
                     RLGLDFRKVDFHCITHFHPDHCVDLIPLLFAMNYTVDFRRSEPLQVVGPAGLRDFYER
                     MRGIFGHWIEARTYPLAFHEAGESRIDFQDFSVETLPMAHAERSIGFRVEAEGRSLVY
                     SGDTDDCLNIVRLGKEADLLILECSFPDERKAPGHLTPALAGRIGREAQCKELLLTHF
                     YPVFQGHDIREECRKEYSGRILLAEDGMKITL"
     gene            complement(1449..2210)
                     /locus_tag="VEL68_20430"
     CDS             complement(1449..2210)
                     /locus_tag="VEL68_20430"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ38406.1"
                     /translation="MKLKGKVAIITGASKGIGKGIAIRYSQEGAAVVLASRSLDLLSA
                     IVDQVRNEGGQAMALDLDVTRYESVEEVVNQTMERFGRLDIMVNNAGISMAKPSEDLS
                     PQEWARALETDLYGVFYGCQCAAKRMIPLGGGTIINITSVYGLVAAPGRAAYCSSKAA
                     ANMLTKVLAIEWAKKNIRVNAIAPGYFRTELVQGVIDSGMLPLGAIERRTPIGRIGEV
                     PELLGLAVYLASDESAYMTGSVINIDGGWEAYGYL"
     gene            complement(2305..3663)
                     /locus_tag="VEL68_20435"
     CDS             complement(2305..3663)
                     /locus_tag="VEL68_20435"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXZ38407.1"
                     /translation="MEAEKGRAERFALAAEKYVPDAITSAVIMVIILFLYAWSIGVPA
                     DKTTDAFYRGLWMLLAFTMQMTLIITLSAVFGQSPIFRKAIMALAKVPNSPFQVVILA
                     ALATAVLGYFYWGLAITLGPVIAVYFCGEAERKGMKVDFMLTLAVVFAAHSVWQYGLS
                     SSAPLLSATPGHFLEKLTGKMPLSTTIWTPAAIFQIVAFMVILLIVARYLVPRVPKTI
                     SEYPEAFKLIEANQAAPAAAEEIKNPTFSQRLERSRLVGFLLVIGILIWLYHHFSTKN
                     LGLDLNSLNAIFLLLCFLLHKNVRNFTLALQEGIKSSWSVVVIYHLYATAAGILQFTP
                     AGDTIAKFMSSISTTYTYPFWMALSGTIIAIFVPSSGGQWVIQGFITSKAAETIGVSF
                     QRGMLSLGVGDQMGNLCSPFWYVIVAGIARVDFRKFFGYGMIFAAIWFILGVLVFTFL
                     PC"
BASE COUNT          866 a         1041 c         1024 g          795 t
ORIGIN      
        1 ggccaacacg tcaacaagac cgagtcagcg gttcgcatca cccacatccc tacgggcatc
       61 gtcgttcaat gccagaatga acggtcccag cacaagaacc gggccatggc catgaaggtg
      121 ctgaaatcac gcctctacga gatgaaatta cgggagcagg aagaaaaggc cgaagccctt
      181 cacagcgcca aaaaggacat cgcctggggc agccagattc gttcctatgt ccttcagccc
      241 taccggctgg tcaaggacca ccgcaccaac tttgagatgg ggaacgtgga cgcggccctg
      301 gacggagact tggatgagtt catccaggcc tatcttctgc agaaggcagc caaaagcgcc
      361 tgaaatcgaa ttcccaaatc ccaagcaccc tataaccaac gggcaccaaa ttccgaatca
      421 gcaaacaacc aagtttcgga cattgaagat tgatggtctc gtaaaaatac aaagatgagt
      481 catttcgagc gaagcgaaac aatcccattg cctttaactc ttggacctca tgagattgat
      541 ttgtcgctgc gctccttaca atgacgcgaa tctgtatttt ttacaatatc atcaagattg
      601 atgcttggaa gttgtttgga atttgggatt tggattttga aatttgtaat ttaggcactc
      661 caagtggaac tcactgtttt gggctcaggt acctgtatcc ccgcggtcga ccgcggccct
      721 tcgggtcttg ctcttacctt cgggtcccac ctgatcctct tcgacggcgg agggggcagc
      781 ctgcgcccga tgccccggct gggcctggac ttccgcaagg tggatttcca ctgcatcacc
      841 cacttccatc ctgaccactg tgttgacctc atccccctgc tcttcgccat gaattacacc
      901 gtggatttcc ggcgttccga gcccctccag gtcgtaggcc ccgcagggct gagggatttt
      961 tatgaaagga tgcgggggat ctttggccac tggattgaag cccgcacgta tcccctcgct
     1021 ttccatgagg cgggggaatc aagaatcgac ttccaggatt tttccgtcga aaccctgccc
     1081 atggcccacg cggagcgcag catcggattt cgggtggagg cggaaggccg ttcgctggtc
     1141 tattccgggg atacggatga ttgtctgaac atcgtaaggc tggggaaaga ggcggacctc
     1201 ctgatcctgg agtgctcttt tcccgacgaa aggaaggcac ccggtcacct caccccggcg
     1261 ctggccggcc gaatcggccg ggaggcccaa tgcaaagagc tcctccttac ccacttctat
     1321 cccgtattcc agggtcacga catccgggag gaatgcagga aggagtattc tggaaggatt
     1381 ctcctggctg aagatgggat gaaaatcacc ctataaggac ggtcctcctt cccgatcggc
     1441 gaagttcatc aaagataccc gtacgcttcc catcccccat cgatgttgat caccgacccg
     1501 gtcatgtagg cagattcgtc gctggccagg tatacggcca agccgaggag ctctggcacc
     1561 tcgccgatcc ttccgatagg ggtgcgtctt tcgatcgccc ccaggggcag cattccgctg
     1621 tcaatcaccc cctgcaccag ttccgtgcgg aagtagcccg gggcaatggc gttgacccga
     1681 atgttcttct tcgcccattc gatggccaga accttcgtga gcatattggc tgccgccttg
     1741 ctggagcagt aggccgccct tccaggggcg gccaccagtc cgtaaaccga agtgatgttg
     1801 atgatcgttc cccctccaag cgggatcatc cgtttggcgg cgcactgaca cccgtaaaaa
     1861 accccgtata ggtccgtttc cagcgcccga gcccattcct gcggggagag gtcctcggag
     1921 ggcttggcca tggagattcc ggcgttgttt accatgatgt cgagacgccc gaatcgctcc
     1981 atcgtttgat tcacgacttc ttccacactc tcataacggg ttacgtccag gtccagggcc
     2041 atcgcttggc ccccttcgtt ccggacctga tcaacaatgg ccgaaaggag gtccaaggaa
     2101 cgactggcca gaactactgc cgccccttcc tggctatagc ggatggcgat ccctttccca
     2161 atccctttgc tggctccagt tatgatggct acttttcctt ttaatttcat tctctttttc
     2221 cttcttctta agaatgtttc taatttcttc cggaagccaa atttccttcc ggcccccgcg
     2281 gaaaaaagaa ggtgatgatt tatcctagca gggaaggaag gtgaagacca aaactcccaa
     2341 aataaaccaa atagcggcga aaatcatccc atagccgaag aactttcgaa aatcgacccg
     2401 ggcaattccg gcgacgataa cataccagaa gggagagcag aggttgccca tctgatcgcc
     2461 cacccccagg gagagcattc cccgctggaa ggagaccccg atcgtttccg cggctttcga
     2521 ggtgatgaac ccttgaatca cccactggcc gccgctggag gggacgaaaa tggcgatgat
     2581 ggttccggaa agggccatcc aaaatggata ggtgtaagtc gttgatatac tggacatgaa
     2641 cttggcaatg gtgtctcccg caggagtgaa ttgcagaatc ccggcggccg ttgcgtagag
     2701 atggtaaatc accaccacgg accaggagga cttgatgcct tcctggaggg ccagggtgaa
     2761 gttgcgcacg ttcttgtgaa gaagaaagca gaggaggaga aagatggcat tcagggagtt
     2821 caggtcgagt cctaaattct tggtggaaaa atgatggtac agccagatca ggatgccgat
     2881 gaccaggagg aaaccgacca gcctgctcct ctccagccgt tgggaaaagg tggggttttt
     2941 gatctcttcg gccgccgccg gagcagcctg attcgcctcg ataagcttaa aggcctcggg
     3001 atattcggag atggtcttgg gcactctggg cacgaggtac cgggcgacga tgagtaaaat
     3061 gaccatgaag gcgacgattt ggaagatcgc tgcgggcgtc cagatggtgg tgctcaaagg
     3121 cattttgcca gtcaattttt ccagaaagtg ccccggggtg gcggaaagca acggcgcgct
     3181 ggaggataaa ccgtactgcc acacggaatg ggcagcaaag acaacggcca gggtgagcat
     3241 gaagtccacc ttcatgccct tgcgctccgc ttccccgcag aaatagacgg cgatgaccgg
     3301 ccccaaggtg attgccaacc cccagtaaaa atatcctagc accgccgtgg ccagggcggc
     3361 aaggatgacc acctggaagg gcgagttggg caccttggcc agggccatga tggccttcct
     3421 aaagatgggc gattgcccga agacggcgct cagggtgatg atcagggtca tctgcatggt
     3481 gaaggccagg agcatccaga gcccccggta aaaggcatcc gtggtcttat ccgcaggcac
     3541 cccgatggac caggcgtaaa gaaagagaat gatcaccatg atcaccgcgg aggtgatggc
     3601 atcggggaca tatttttctg ccgctaaggc aaaacgctct gcacggccct tttccgcttc
     3661 catagaatcc tccttttcgt taaattgaag cactggaaat taccgcttcg gtgcttgggt
     3721 ggaacc
//
LOCUS       DASPRR010000555         9228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1101477, whole genome shotgun sequence.
ACCESSION   DASPRR010000555 DASPRR010000000
VERSION     DASPRR010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101477"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VEL68_20440"
     CDS             <1..1019
                     /locus_tag="VEL68_20440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ38408.1"
                     /translation="SEASRASYLDFGLHLGLFQVERQYQQIPDAFRFGVRTFKMFMTY
                     SKLGRFTSDYYLTASFDLIGKHGGMAMVHAENGLATDYLEDKFNSQGVSAIDAFTRMR
                     PAALEGEAIHRAIAIAEICQCPIYIPHVSCNRGLEPIAQARARGQKVYAETCPQYLEL
                     TEKDLYRWKALAKIGPPLRTPEDNEALWMGISKGIIEVVASDHAPKAKKVDEDFFNAA
                     YGSPQAETLLNVTYDAGCNRNRISLPRLVQVLSENPARVFGLYPRKGCLQEGADADLV
                     VYDPSARHTLTGKSLHSKAGYTLFEGRECLGKPWITFQRGRQIFDRMQVVARPGDGKY
                     LRLA"
     gene            1201..1893
                     /gene="pyrE"
                     /locus_tag="VEL68_20445"
     CDS             1201..1893
                     /gene="pyrE"
                     /locus_tag="VEL68_20445"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXZ38409.1"
                     /translation="MILRLEEIERKVWEEEKLFLMKQVGILLIKNNAVKFGDYILTSG
                     KKSPYYIDLRQTISSPITMDWIANSLTRIVVNEIGISRIDKILGVPTAGVPFATVVSQ
                     KLGIPLIYYRQARKEHGVRKKVEGILERNDRVLIVDDLITTGESVIEAAEVVRDQGGV
                     VNEMVVLLDREQGGEENLRKSRIEPHLLFKISAAMNWLHSVRLISDDIYETLASYLEE
                     EKKASVKAAAGG"
     gene            2130..3614
                     /gene="lysS"
                     /locus_tag="VEL68_20450"
     CDS             2130..3614
                     /gene="lysS"
                     /locus_tag="VEL68_20450"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXZ38410.1"
                     /translation="MEELKDLFLQRKKKFEELIQEGFPPYPNTFRISHSAEEIARLYG
                     DRSEEEFKTLTERFSVAGRLVRLNHFGKAAFGHIQDRTGRIQGYFRRDQMSPGSFEVF
                     SRLDIGDIVGIQGKLFRTRTGELTLMVEEAELLAKSFRPLPEKWHGLTDLETRYRQRY
                     VDLIMNPHSRDVFIIRSRTVDFLRRFLTQRGFLEVETPMMQPIPGGATARPFKTHHNT
                     LDMDLYLRVAPELYLKRLVVGGLERVFEVNRNFRNEGISTQHNPEFTMLEFYQAYATY
                     RDLMDLTENMFSLLVKEIKGGTSLDYQGTSIDFRSPWRRLSIQEGLTAYGELKEEELK
                     DREKVLAYGRKAGLELKGDEPLGKVWVDLFEKIAEPNLIQPTFVTEYPIEDSPLARRN
                     DQKPEFVDRFELYIFGREIANAFSELNDPQDQKARFLEQAKAKARGDEGAGLMDEDYV
                     RALEYGMPPAAGEGIGVDRLVMLLTDSPSIRDVILFPQMRPEKE"
     gene            3710..4942
                     /locus_tag="VEL68_20455"
     CDS             3710..4942
                     /locus_tag="VEL68_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HXZ38411.1"
                     /translation="MRYELFISLRYLKAKRKQVFISVITFLSMAGVGLGVTALVVVLS
                     VMRGFEDDLKSKILGTNAHLVILQHGTPIRDYREIIQKVKGVEGVVAATPFIFTQAMI
                     TSESNAYGIVLRGIDPATAGQVINIETTLKKGTLDSLKEKEDSSAHPGIFIGKELAQN
                     LGVMLDDTVVVVSPTGALAPMGPGPPMKKFRIKGIFDSGMYEYDTSLAYISLKSAQKF
                     LGMEDAVSGVEVKVQDLYGVKEIARKVQKELGFPFWTKDWMQMNRSLFAALKLERTVM
                     FIILVLIVLVAAFGIVSTLIMVVMEKNKDIAILKSMGATARSIMRVFILEGVIIGVFG
                     TALGLIGGYGICRILATYQFISLPRDVYYISHLPVKMSGIDFFLIALSAMGISFLATL
                     YPSWQASKLDPAEALRYE"
     gene            5015..6220
                     /locus_tag="VEL68_20460"
     CDS             5015..6220
                     /locus_tag="VEL68_20460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXZ38412.1"
                     /translation="MAGFWEGLFSGGKYEQELKEVQEKAAREPKNLRLRLRMGDLLEK
                     LGKRAEAVETYRTVAEEYAKKGLLIQAIAVHKLILRLDPTKSELQEQIADLYAQWGRA
                     GEEMPGRVEEKGEIPPPKAEKLPLIPLFSDLKKEELSRVMEKIQAKWFGKGKPICREG
                     DPGNSIFIISLGKVAVLRQQPGRGKTKLAELKEGDFFGEFGYFSNSRRQATVEALEDT
                     EVLELTKEELQKIVREFPMVSQVLFKFYKERVLDNLLATSALFQDFPPEERRQVLDHL
                     SLKEIPAGTRLIQEGAPGDCMYIIKSGEVEVSTSDPKGQKLVLATLQEGDFFGEISLI
                     TGHARTASVTTLRATELVCLGKEDFDRFIPRHPETLKVLEESLQTRLENKLRMLGVFR
                     KSPAKEGMI"
     gene            6250..6924
                     /locus_tag="VEL68_20465"
     CDS             6250..6924
                     /locus_tag="VEL68_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38413.1"
                     /translation="MIQAQNLKKSFLFGASGKVIDVLRGIDLTVRAGEMLAVEGASGA
                     GKSTLLHILGTLDRPTEGKVLFEGQDLFDRTEGELAELRNRHIGFVFQLHHLLPEFSA
                     LENALMPGLIQGWALKKAGAEAERILVQLGLKDRLHHKPGQLSGGEQQRVAVARALML
                     NPKMILADEPTGNLDTQIGEEVHNLLVRINREQGITIIIVTHNPFLAARMTRRVRLSD
                     GRVQES"
     gene            7032..>9228
                     /gene="bamA"
                     /locus_tag="VEL68_20470"
     CDS             7032..>9228
                     /gene="bamA"
                     /locus_tag="VEL68_20470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXZ38414.1"
                     /translation="MNKGLLLRFFLLFTLSWAWIPGKTQAQEAVRVGILPFRIYAADQ
                     EKGLAWSSRVAGTLGAELKKDEKILLVGEEKIRAALQEAGAAEVDESLAREIGQKVEA
                     DYMVLGSVTQIDGSISLDIRIVDVFEREALTSIFRVGKSSEELDTITRQASQDVKWKV
                     LKKEILAKVLIEGNRAIEESAIRAQIKLKEGDAFSPPMVREDLKALYQMGFFQDVRVE
                     KRDWERGKALVFVVEEKPIVKEVVFTGNKELKTSDLLEVVDIKPRSVLNLNAVKESVN
                     KILKKYREEAYFAAEVLYELESATRKGEVIVHFRIKENEKVRIGKISFSGNAHYSDEI
                     LKKVLPETKEEGWFSWVTKSGTYKEDILDRDLDAIGAFYLQKGFMQVKLGKPKVTIDK
                     KGIEITIPVEEGQQFKIGKVDIQGDLILPKEELLKRVPIYVGEILNRDHVREALSNLT
                     DLYADKGYAFVDVDPMTVPYKDQPLVDLTFQIHQGSKVYFERINILGNTKTRDRVIRR
                     DLQTVEGDLYSLTAVKKSREALNRLGYFKEVNVNTKKGSAEDRMVVNVQVEESPTGSF
                     SGGVGYSSIDKLVTVFSISQNNLFGRGQRIMGQAQIGSISKLYNISFTEPRLFDTQIM
                     TGADIYNIYRDYTDYSVKRKGGVVRFGFPIVEYLRGVLQYKYEQADTYNVKANSSQII
                     LDAQGLSTTSSITGVLRRDARDNYIDPTQGSDNTLSMEYAGGFLGGSNEF"
BASE COUNT         2245 a         2408 c         2675 g         1900 t
ORIGIN      
        1 tttccgaagc aagccgggct tcctacctgg atttcgggct gcacctgggc ctctttcagg
       61 tggagaggca ataccagcag attcccgatg cctttcgctt cggggtgcgg accttcaaga
      121 tgttcatgac ctattccaag ctcggacggt tcacctccga ttattatttg actgcctcct
      181 ttgacctgat cggcaaacac ggaggcatgg ccatggttca cgccgaaaac ggcctcgcca
      241 ccgactattt ggaagacaag ttcaacagcc agggagtttc agcgatcgat gccttcactc
      301 gtatgcgtcc ggcagccctg gaaggggaag ccatccatcg ggccattgcc atcgcggaaa
      361 tatgccaatg cccgatttac atcccacatg tcagctgcaa ccgggggctg gaacccatcg
      421 ctcaggcccg ggcccggggt cagaaggtgt atgctgagac ctgcccgcaa tatctggagc
      481 ttaccgaaaa agatttatat cgctggaaag cgttggctaa aatcggtcct cctctacgga
      541 caccggagga taacgaagcc ctctggatgg ggatttccaa agggattata gaagtagtgg
      601 ccagtgatca tgcgcccaag gccaagaagg tcgatgaaga cttcttcaac gctgcatatg
      661 gttcccctca ggcggaaacc cttctgaacg taacctatga tgcgggatgc aaccgtaacc
      721 gcatcagcct tcccaggctg gttcaggttc tttccgaaaa tccagcccgg gtcttcggtc
      781 tctatccacg caagggatgc ctgcaggaag gggcagatgc tgatttggtc gtttacgatc
      841 cctcagcccg gcacaccctg accggaaaat ccctgcactc caaggccggc tacacgctat
      901 ttgaagggcg tgaatgcctg ggaaagccct ggataacatt tcagcggggc cgccagattt
      961 tcgaccggat gcaggttgtg gcccggccgg gggacggaaa atatctgcgc ctggcctagg
     1021 attccctccc gccgaacaga aaaaatttcg cttgaagcca ggggcgggcc ggtccggcaa
     1081 gcggagtttc gcccccggaa aaaaataaat atttttgcgt tccggcaacg gctggaaatt
     1141 ttgttgactt ttctcccccg ctcggctaaa aaagaaagca aaaattaggg gggctagaat
     1201 atgatcctga ggctcgaaga aatcgaaagg aaggtttggg aagaagaaaa actcttcctc
     1261 atgaaacagg tcggcatcct tctaattaaa aacaacgcgg tcaaattcgg agattacatt
     1321 ttgacctccg gaaagaaaag cccctattac atcgacctgc ggcagaccat ttccagcccg
     1381 atcaccatgg actggattgc caattccctg acccggattg tggtgaatga aatcggcata
     1441 agccgcattg ataagattct cggggttcca acagccgggg ttccttttgc cacggtggtc
     1501 agccagaaat tggggattcc tctgatctat taccggcagg ccaggaaaga acacggggtt
     1561 cggaaaaagg tggaagggat cctggagcga aatgatcgag tgctgatcgt cgatgatctg
     1621 atcaccaccg gggaaagcgt catcgaggcc gccgaagtcg tccgggatca ggggggcgtg
     1681 gtgaatgaaa tggtggtcct cctcgaccgg gaacaggggg gagaggaaaa tttgcgcaaa
     1741 tcccggattg aacctcatct cttgttcaaa atttccgctg ccatgaattg gctccattcc
     1801 gtccgtctga tcagcgacga catttatgag acgctcgcca gttacttgga ggaagaaaaa
     1861 aaagccagcg tcaaagccgc cgccggaggg tagagaaatc agccggccaa ttcgaacccg
     1921 gcctctccct ggggggccga catagatccc gtgcctgcca gaaacactat tcctttcaag
     1981 gaattttgcc ccaagtccgc caacatccgg gcttgactcc agggattttt taagctattt
     2041 ttaggactcg ataccttcgg gtaaatacgc caagaatcaa aggttccgag ggatacccct
     2101 gggccttttt tcttttagaa gagtggaacg tggaggagtt aaaggacctt tttttacagc
     2161 gaaaaaagaa gttcgaggaa ttaatccaag aagggtttcc cccttatcct aacacttttc
     2221 ggatcagcca ttccgcagag gagatcgccc ggctctacgg ggatcgaagt gaagaggaat
     2281 tcaagaccct aaccgagcgt ttctctgtgg ccgggcgtct ggtgcgcttg aatcattttg
     2341 gcaaggcggc tttcggccac atccaggatc ggaccggaag gatccagggt tatttccggc
     2401 gggatcagat gagcccgggg agctttgagg tgttctctcg cctggatatc ggggatatcg
     2461 tgggtattca gggaaaactc ttccgcaccc gcaccgggga actcaccctg atggtggagg
     2521 aagcggagct tctggccaag tccttccgcc cccttccaga aaaatggcat ggcctcaccg
     2581 acctggaaac ccggtaccgg caacgttatg tggacctcat catgaatccg cattcccggg
     2641 atgtttttat catccgcagc cggacggtgg atttcctccg aaggtttctc acccagcggg
     2701 gctttctcga agtggagacg cccatgatgc agcccatccc cgggggagcc acggcacgtc
     2761 ctttcaagac ccaccacaat acgctggaca tggacttata cctccgggtg gccccggagc
     2821 tttacctgaa acgcctagtc gtgggcggat tggaaagggt cttcgaggtc aaccggaact
     2881 tccgcaacga agggatttcc acccagcaca accccgaatt caccatgctc gaattctacc
     2941 aggcctatgc cacttaccgc gacctgatgg acttgaccga gaacatgttc tcattactgg
     3001 ttaaagagat caaagggggg acgtccctgg attaccaggg aacgagcatc gacttccggt
     3061 ccccctggag aaggctcagc atccaggagg gcctgactgc ttatggggaa ttgaaggaag
     3121 aggagctgaa ggaccgggag aaagtcctgg cttatggaag aaaggcggga ctggagctga
     3181 agggggatga accgctgggg aaggtatggg tggacctttt cgagaagatc gccgagccca
     3241 acctgattca gcccactttt gtcaccgagt atccgattga ggattcgccc ctggcgcgga
     3301 ggaacgacca aaagccggag ttcgtggacc ggttcgagct gtacatcttc gggagagaaa
     3361 tcgccaacgc cttctcggag ctcaacgatc cccaggacca gaaggcccga ttcctggaac
     3421 aggccaaggc taaagcccgg ggggatgaag gagccgggtt aatggatgag gactacgtgc
     3481 gggccctgga atacgggatg ccgcccgccg ccggggaggg gatcggcgtc gaccgcctgg
     3541 tcatgctcct gaccgattca ccctcgatcc gggacgtcat cctctttccc caaatgcggc
     3601 cggagaaaga atagctttca gcggtcagca atcagctttc agctcaaaac aaaacccggc
     3661 tgatggctga aagctgatgg ctgaaagctg atcgctgaaa gctgttttta tgcgctatga
     3721 actttttatc agtcttcgct acctgaaggc caaaaggaaa caggtcttta tatccgtgat
     3781 caccttcctg tccatggccg gggtcggctt gggggtcacg gccctggtgg tggttctgtc
     3841 ggtcatgcgg gggtttgagg acgacctaaa aagcaagatc ctgggcacca acgcccatct
     3901 ggtgatcctc cagcatggca ccccgattcg agactatcgg gaaatcattc aaaaggtaaa
     3961 gggcgttgag ggggtggtgg cagccactcc ctttatcttc actcaggcga tgattacttc
     4021 ggaaagcaac gcctatggga tcgtcctgcg gggaatcgac ccggctacgg cagggcaggt
     4081 cattaatatc gagacgaccc tcaaaaaggg caccctcgac agcctgaagg aaaaggagga
     4141 ttcttcagcc catccaggga tcttcatcgg aaaggaactt gctcagaacc tgggggtcat
     4201 gctggacgat acggtggtcg tggtttcccc caccggggct ctggctccca tgggcccggg
     4261 accgccgatg aaaaaattca ggatcaaggg gatcttcgat tcaggcatgt atgagtatga
     4321 cacttccctg gcttatatct ctttgaagag cgcccagaaa ttcctgggca tggaggacgc
     4381 cgtctccgga gtggaggtga aggttcagga cctctacggg gtgaaggaga tcgcccggaa
     4441 ggtccagaag gagctgggct ttcccttctg gaccaaagac tggatgcaga tgaaccggag
     4501 cctcttcgcc gccctgaagc tggagcggac ggtcatgttc atcattcttg tgctcatcgt
     4561 cctggtagcg gccttcggca tcgtaagcac cctgatcatg gtggtcatgg agaagaacaa
     4621 agatatcgcc atcctgaagt ccatgggagc cactgcccgt tcgatcatgc gggtttttat
     4681 ccttgaggga gtgatcatcg gggtcttcgg gacggctctc ggcctgatcg ggggttatgg
     4741 gatctgccgg attctggcca cctatcagtt tatctctctg ccccgggacg tttactacat
     4801 ctcccacctt ccggtgaaaa tgagcggaat cgattttttc ctcatcgccc tgtcggccat
     4861 gggaatcagc ttcctggcga ctctataccc ttcctggcaa gcctccaagc tcgacccggc
     4921 ggaggccttg cggtatgaat aaaaagggag ggcggtgaag acttctttct cagcccaaag
     4981 gtccggcgtt cgtttcccac caaaggaggt ttagatggct ggattctggg aaggattgtt
     5041 ttccggggga aaatacgaac aggagctgaa agaggtccag gaaaaggcgg ctcgggagcc
     5101 gaaaaacctt cgcctccggc tccgcatggg ggacctgctg gaaaagctgg ggaaaagggc
     5161 ggaagccgtc gagacctacc gaaccgttgc cgaggagtac gccaagaaag ggctcttgat
     5221 ccaggccatt gccgtccata agcttatcct acgcttggac ccgacaaaat ctgagcttca
     5281 agagcagatc gctgacctct acgctcaatg gggaagggcg ggggaggaaa tgccgggaag
     5341 ggtagaggag aagggtgaga ttccccctcc caaagccgag aaacttccgc tgattcctct
     5401 cttttcggac ctgaaaaaag aagagctttc ccgagtcatg gaaaaaattc aagccaagtg
     5461 gttcggcaaa gggaaaccaa tttgtagaga aggggacccg ggtaattcaa ttttcatcat
     5521 aagccttgga aaggtggccg tcttacggca acaacccggc agaggaaaga ccaaacttgc
     5581 cgagctcaaa gaaggggact tctttgggga attcggctat ttctccaatt cccgaaggca
     5641 ggccaccgtg gaggccctgg aagacaccga ggtcctggag ttgaccaaag aggaattgca
     5701 gaagatcgtc cgggagttcc ccatggtctc tcaggttctc tttaaattct acaaagagag
     5761 ggttctggac aatctactgg ccacttccgc cctcttccaa gactttcctc ctgaagagag
     5821 aaggcaggtc ctggaccatc tgagcctgaa agagattcct gcgggcaccc ggctgatcca
     5881 agaaggcgcc cccggagact gcatgtacat tattaaaagc ggggaggtgg aggtctccac
     5941 ttccgacccc aaggggcaga agctggtttt agcgaccctc caggaggggg atttcttcgg
     6001 ggagatctcc ctgattacgg gacatgcgcg gaccgcttcg gtgactacct tgcgggctac
     6061 tgagctggtc tgcctgggga aagaggattt cgaccggttc atcccccgac accccgagac
     6121 gttgaaggtc ctggaagaat ctcttcaaac caggctggag aataagcttc gcatgctggg
     6181 agtcttccgc aaaagcccag ccaaggaggg aatgatctga acccgtcaag ccctgccatt
     6241 aaccttcccc tgattcaagc ccaaaatctg aagaaatcct ttctcttcgg ggcaagtggt
     6301 aaagtcatcg atgtcctgag ggggatcgat ttgaccgtcc gggccggaga gatgctggca
     6361 gtggaagggg cttccggcgc aggcaagagc accctcctgc acatcctggg gaccttggat
     6421 cggcccaccg aaggaaaagt gcttttcgaa gggcaagacc ttttcgatcg gacggaaggt
     6481 gaactagcgg agttgcgcaa ccgccacata ggctttgtct tccagctcca ccatctcctt
     6541 ccagaattca gcgccctgga aaatgccctg atgcccgggt tgattcaggg gtgggcattg
     6601 aaaaaggcgg gagccgaggc ggaaaggatt ttggtccagt tggggcttaa agaccggctc
     6661 caccataaac ccggccaact ctccgggggc gagcagcaga gggtggcagt ggcccgggca
     6721 ctgatgttga atccgaaaat gatcctggcc gatgaaccca cgggaaacct ggatacccag
     6781 atcggggaag aggtgcacaa tctcctggtc cggatcaacc gggaacaggg gattaccatc
     6841 attatcgtga cccataaccc ctttttggca gcccgcatga ctcggcgggt ccgtctgagc
     6901 gacggccggg tgcaggaaag ctgatctgcg aatccggagc ctgcaggcag ggaaggagat
     6961 gaggctccca aattttcaag gagaggtgaa ggggcttcga acccgagggt cagaagccga
     7021 tgagaccgcc catgaacaaa ggccttctgc tccgattctt tctccttttc actttgtcct
     7081 gggcttggat acccggcaaa acccaggcgc aagaggcggt cagagtggga atccttccct
     7141 tccgcatata cgcggcggac caggaaaaag gcttggcctg gtcaagccgg gtggcaggga
     7201 ctctgggcgc tgagttgaag aaagatgaga agattcttct ggtgggagag gaaaaaattc
     7261 gggcggctct gcaggaggcc ggggcagccg aggtggatga gtccctggcc cgggagatcg
     7321 gacaaaaggt ggaagccgac tacatggtgc ttggttcggt gacccagatc gacgggtcca
     7381 tcagtctgga catccggatc gtggacgtct ttgagcggga agctttaacc tcgatcttcc
     7441 gggtgggtaa atcatcggaa gaactggata ccataacccg ccaagccagc caggacgtaa
     7501 aatggaaagt cctcaagaaa gaaatcttgg ccaaggtcct catcgaaggc aaccgggcca
     7561 tcgaagagag cgccatccgg gcccagatca agctcaagga aggggacgcc ttttctccgc
     7621 caatggttcg ggaggacttg aaggccctct accagatggg ttttttccag gacgtaaggg
     7681 tggagaaacg cgattgggag cgggggaaag ccctggtttt cgtggtggag gagaagccga
     7741 tcgtcaagga agtcgtcttt accgggaata aggagctcaa gaccagtgac cttctggaag
     7801 tagtggacat caagcccagg tcggtgctga atctcaacgc ggtcaaggaa agcgtaaaca
     7861 agatcctcaa gaaataccgg gaggaggcct acttcgctgc ggaagtcctc tatgagctgg
     7921 agtcggccac ccgcaaaggg gaagtcattg tccatttccg aatcaaggag aatgaaaagg
     7981 tccgcattgg caagatctcc ttttccggca atgcccacta cagcgacgaa attctgaaga
     8041 aagttctgcc ggagaccaag gaggagggtt ggttttcctg ggttaccaaa tccggaacct
     8101 acaaggaaga cattctggat cgggaccttg acgctatcgg cgccttttac ctccagaagg
     8161 gttttatgca ggtcaagctg ggaaagccta aggtgacgat cgacaagaaa ggaatcgaga
     8221 tcaccatccc ggtggaagaa ggtcaacagt ttaaaatcgg gaaggtggac atccaggggg
     8281 acctgatctt gcccaaggag gagttgttga agcgggttcc gatctacgtg ggggaaatct
     8341 taaaccggga ccatgtccgc gaagccctgt ccaaccttac ggacctgtac gccgacaagg
     8401 gatacgcctt tgtcgatgtg gaccccatga ccgttcctta taaagaccag cccctggtgg
     8461 acctgacttt ccaaatccat cagggctcca aagtgtattt cgagcggatc aacattctcg
     8521 gaaacacgaa aacccgcgac cgggtcatcc gtcgagacct ccaaaccgtg gaaggagacc
     8581 tctatagttt gaccgccgtg aaaaaaagcc gggaggcact gaacaggctg gggtatttca
     8641 aggaagtgaa tgtaaacacc aaaaagggga gcgccgaaga tcgaatggtg gtcaatgttc
     8701 aggtggaaga atctcccacg gggtccttca gcggcggggt ggggtacagc tccatcgaca
     8761 agctggtgac cgttttctcc atttcccaga ataacctctt cggccgggga caacggatta
     8821 tgggccaggc ccagatcggt tccatttcca agttgtacaa catcagtttc accgagcccc
     8881 ggctcttcga cacccagatc atgactggag ccgatatcta caacatctac cgcgactaca
     8941 ccgactattc ggtcaagaga aaaggcgggg tggttcggtt tggctttccc atcgtcgagt
     9001 acctacgggg agtcctccag tacaaatacg agcaggcgga cacctacaac gtcaaagcga
     9061 attcctccca aattatttta gacgcgcaag ggcttagcac gaccagcagt attaccgggg
     9121 ttctccggcg ggatgcaagg gataactaca ttgatcccac ccagggatcg gataacactc
     9181 tttccatgga gtacgccggc gggtttctcg ggggaagcaa cgaattca
//
LOCUS       DASPRR010000556         5818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_789320, whole genome shotgun sequence.
ACCESSION   DASPRR010000556 DASPRR010000000
VERSION     DASPRR010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789320"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..516)
                     /locus_tag="VEL68_20475"
     CDS             complement(223..516)
                     /locus_tag="VEL68_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HXZ38415.1"
                     /translation="MAKTKTRPWDAAENLETEEDMALYLEAALEDGDPAVVAAALGDI
                     ARAKGMSEIARETGLGRESLYKALSPEGNPEFATVLKVIRALGLRLHATAEHA"
     gene            complement(526..816)
                     /locus_tag="VEL68_20480"
     CDS             complement(526..816)
                     /locus_tag="VEL68_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXZ38416.1"
                     /translation="MIEIRQTEVYARWFNGLRDRQARARINVRIRRLSLGNPGDVKPV
                     GEGISELRIDYGPGYRVYYVQRGQTLVILLAGGEKHTQDKDIKTALELAREL"
     gene            complement(1323..2747)
                     /locus_tag="VEL68_20485"
     CDS             complement(1323..2747)
                     /locus_tag="VEL68_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014113016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HXZ38417.1"
                     /translation="MNDQGKNVSGRLIRGILVILVGVIIWNLPIPAGVKPDAWHLLAI
                     FVATIFGLILSPLPMGAVVIIGVMMTTLTGILKIGPALAGFANSTVWLIVAAFLVARG
                     FIITGLGTRIAYMFIRSFGKKTLGLAYSIAASDLVLSPATPSNTARAGGIIYHIVRSL
                     CSTFGSEPGETARKIGAYLLMVEFQATIITSVLFMTAMAPNPLMVELAQKTANVTLSW
                     GRWTLISCVPTLLSIFFIPYFLYKIFPPEIKATPQAAEMAHRELTKMGPWKRTEKTML
                     IVFLLILVLWATSEWNKIDATIVALIGIGVMLVTKVITWDDILAERGAWDALIWFGGL
                     VTMAGQLNTLGLIKWFATVMGGVVAGWGWLPALIVLLLVYVYAHYGFASTTAHVTAMF
                     PPFLAVMIGAGAPPYLAAMSLAFFSGIMNGITHYGTGPAPIYFNAGYMDQKTWWLLGL
                     VISLINIGIFLTAGFAWWKFLGLW"
     gene            complement(2946..4379)
                     /locus_tag="VEL68_20490"
     CDS             complement(2946..4379)
                     /locus_tag="VEL68_20490"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HXZ38418.1"
                     /translation="MIFASRRIIPLISLWVLFFVWAPGAAGAETSTLKDQQSVALTIY
                     NSNVGVVKETRLINLPQGIQTLRFTDVPGRIDPATVNLKSLAEAGSLRVLEQNFEYDL
                     VSQQKLLEKFVGQKVDLVWLNPDSKKEETVEATLLSTQGGNIFQIGDRVSLGHPGRIV
                     LSKIPEGLSLQPSLFWLLENRLGGPQRLEVTYLTSGINWKADYLALLNPADTHLDLTG
                     SVTIENRSGAGYRNAILRLVAGEVQRVREEKISDRAAPRIAAAAQEAAPQFKEESFFE
                     YHLYTLDRGTTLRDNQTKQLLLFSAPQVPVSKLYLLQGQPHYYWSRYDLRGQKPRVGV
                     YLEMVNRQENRLGMPLPKGTVRVYKADQDGTLQFAGEDRIDHTPRNEKFKVKIGEVFD
                     VAAERVQTDFKQLEPRVSESSFEVTLRNQKNEDIRVLVEEPIPGAWEIISNTHPYEKI
                     QANLIRFNVPVTKGQEVKVRYKVRMRY"
     gene            complement(4418..5176)
                     /locus_tag="VEL68_20495"
     CDS             complement(4418..5176)
                     /locus_tag="VEL68_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HXZ38419.1"
                     /translation="MRALPISLGGLAAFGVGTFILLCPASPQQGLILEAGKFSAAEAG
                     GSFPAGWKPLTFKNIDKHTLYSLVKDQNVVVVKAESQASASGLIREIKIDPREFPILQ
                     WRWKVENVLKKGDVRRKEGDDYPARIYITFEYDPQKLSFLDRTKYGLVKAFYGQYPPL
                     AALNYIWESQAPPGTLVPNPFTDRVMMIVVESGAEKLNQWVTEERNVLEDYRKAFGED
                     PPLINGVAIMTDTDNTGESAVAYYGDIWFKKKEP"
     gene            complement(5294..5635)
                     /locus_tag="VEL68_20500"
     CDS             complement(5294..5635)
                     /locus_tag="VEL68_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXZ38420.1"
                     /translation="MKLIIRLLINMVAILAIAYLFPSLMRVETIWAAVGAAILLGIVN
                     AIVRPIFVLLTLPLTILTLGLFLLVINALLLWMVSGLVGGFQVNGFWGSFFGSLLISF
                     ISWVLSRLIVN"
BASE COUNT         1344 a         1619 c         1578 g         1277 t
ORIGIN      
        1 ccattctcaa aacctggacc tcaatccaag gaaaggttat aggggaaatc cagaacttac
       61 cgaatcataa cagaaaatta cattcaggtc aaaaatactg taaggcgaga gttgtgagga
      121 gtgagtggtg ggaaaaaagg aatgccgacc aaccggcgca agacgaaagg gtaaatcgca
      181 ggggcatagc ggaaaagggt ccacggattt ggggggtaaa gcttatgcgt gttcagcggt
      241 agcgtgcaag cggagaccaa gcgccctgat gaccttaagc accgtggcga attccggatt
      301 gccttcagga gacaatgcct tataaaggct ctcgcgcccg agacccgttt cgcgggcaat
      361 ctccgacatc cccttggcgc gggcgatgtc ccccaacgca gccgccacca ccgcagggtc
      421 gccgtcttcg agcgcagctt ccagatagag ggccatgtcc tcttcggttt ccagattttc
      481 cgctgcgtcc cagggtctgg ttttagtctt tgccatgaca gtctcctata gttctcgcgc
      541 cagctccagc gccgtcttga tgtccttgtc ttgagtatgc ttttcgccgc cggcaagcaa
      601 gatcaccagc gtctgccccc gctgtacgta ataaacccgg taacctgggc cgtagtcaat
      661 gcgaagttcc gagatccctt ctcccacagg cttcacgtcg ccagggttgc ccagtgacag
      721 gcgacggatg cgaacattga tgcgcgcccg ggcttgccga tcacgcaagc cgttgaacca
      781 ccgagcgtac acctcggtct ggcggatctc tatcatgatt taatagtatc tcataggata
      841 cataaaagca agaagaattt cttgtccatc ctccgttcgc ccccccccac tggcatgccc
      901 ccgcgtcggg ccaggacgag aggagatctg cctccactag ctcccgccaa tcccgatcaa
      961 tgtcgatgat ctttgtgggc tgtcgccagt ggtacgtccg ctagaacaag attcattccc
     1021 ccccaaacac gcctcctaac cctccccatc agaagttgaa aaagacattc gcaaggtacc
     1081 ggtgccccgg tgttcaatca gccgcgcccg gtagagcgcg tgtgcaagat agggatggat
     1141 aggatgtagg agaacaaaaa taccgctgtc catacgcccg caggccagac agggggaaga
     1201 gggttcgtgg cctctatttc tctgcgcaat ctgcgtaatc tgcggataaa taggatgtcc
     1261 taagaaaaaa aggaggcgga atgtggattc cgcctccctg cctctgatcg ttgccttccc
     1321 ttctaccaca atccgaggaa cttccaccag gcaaatcccg ccgtcaaaaa gatgccgata
     1381 ttgatgaggg agatgaccag acccaggagc caccaggttt tttgatccat gtatccggcg
     1441 ttgaaataga tgggagcggg gccggtgccg tagtgggtga tcccgttcat gatcccggag
     1501 aaaaatgcca ggctcatggc cgccaggtag ggcggtgccc cggctccaat catgaccgct
     1561 aggaacggtg gaaacatggc ggttacatgg gccgtggtac ttgcgaagcc gtaatgggca
     1621 taaacataga ccaacaggag cacaatcagg gccgggagcc aaccccaacc ggccactact
     1681 cctcccatga ccgtagcaaa ccatttgatc aacccgaggg tgttcaactg tccggccatg
     1741 gtcaccaacc ctccgaacca gatcaaagca tcccaggctc ctctctcggc caggatgtca
     1801 tcccaggtaa tcactttggt gaccagcatc actcctatgc cgatcagggc cacgatggtc
     1861 gcgtcgatct tgttccattc cgaggttgcc cagaggacca ggatcaagag gaagacgatg
     1921 agcatggtct tctccgtccg tttccaaggc cccattttcg tcagctcccg gtgagccatc
     1981 tctgccgcct ggggcgtggc cttgatctcg gggggaaaaa tcttgtaaag aaaataggga
     2041 atgaagaaga tcgacaacaa tgtaggtaca caggagatca gcgtccatcg gccccaggac
     2101 agggtgacat tggccgtctt ctgggcaagc tcgaccatca gcggattggg cgccatggcg
     2161 gtcataaaga ggacggaggt gataatggta gcctgaaact cgaccatcaa gaggtaggct
     2221 ccgatcttcc tcgccgtctc ccccggctcg gagccgaagg tgctgcagag cgaccggacg
     2281 atgtgataga tgatcccccc ggccctcgcc gtattcgagg gggtggccgg cgaaaggacc
     2341 aggtcgctgg ccgcgatgga ataggctagc cccagggtct ttttgccgaa ggagcggatg
     2401 aacatgtagg cgatccgggt gcccaagccc gtaatgataa atccccgggc caccaggaag
     2461 gcggccacga tgagccagac cgtgctattg gcaaatcccg ccagcgccgg gcctattttc
     2521 aggatcccgg tcaaggtggt catcatgact ccgatgatga ctacggcccc catgggcagc
     2581 gggctgagaa tcaacccgaa gatggtggcc acaaagatgg ccagcaaatg ccaggcgtcc
     2641 ggcttgactc ccgcaggtat gggcaggttc cagatgatca ctcccacgag gatgacgaga
     2701 atcccccgta tgagtcgacc actcacgttt ttcccttgat cattcattct tttctccctg
     2761 tcgtgctatt tgtgtatctc gattccaaaa ccaagcctac ctttttaatt aaaagggagt
     2821 tccctcccat gacaactgtt atattaatac aaacgcatta aataaaatga catcaccccc
     2881 accctaaccc tccccccttg agggggaggg gagggtgggg ggtaaaataa tatatttcgt
     2941 ttgtattagt accgcatgcg tactttgtac cggaccttga cttcctgtcc cttggtcaca
     3001 ggcacattaa agcggatcaa gttcgcctga atcttttcgt aaggatgggt gttggagatt
     3061 atctcccagg caccgggaat gggctcttct accaagactc ggatgtcttc attcttctgg
     3121 ttccttaggg tgacttcgaa gctcgattca gagacccggg gttccagctg tttgaagtcg
     3181 gtctgcaccc tctcggcagc cacatcgaag acctccccga tcttgacctt gaatttttca
     3241 tttctggggg tgtgatcgat ccggtcttcc ccggcgaact gcagcgttcc gtcttggtcg
     3301 gctttgtaga cccgaaccgt acccttgggc aggggcattc ccaggcggtt ctcttgccgg
     3361 ttcaccattt ccagataaac tcctactcgt ggcttctggc cccgcaggtc ataacgggac
     3421 cagtagtaat ggggctgccc ctgaaggaga tacaatttac tcacagggac ctggggagcg
     3481 ctgaaaagaa gaagctgttt ggtctgattg tcgcggaggg tggtcccccg gtccagggta
     3541 tagaggtggt attcgaagaa agactcctct ttgaactggg gagcggcctc ctgggcggct
     3601 gcggctatcc ggggagcggc cctgtccgat atcttctctt cccggacgcg ttggacttcc
     3661 ccggccacca gtctcaggat tgcatttcga tagccggccc cgcttcggtt ctcaatggtc
     3721 actgatccgg tcaaatccag gtgcgtatcc gctggattca gaagggccag ataatcggcc
     3781 ttccagttga ttcccgacgt caggtaggtg acctccagcc tttgaggccc gcccagtctg
     3841 ttctccagca gccagaacaa ggacggctgg aggctcagtc cttcgggaat tttggaaaga
     3901 acgattcgcc cggggtgtcc cagggagacc cggtccccga tctgaaagat gttcccaccc
     3961 tgggtggaga gaagggtggc ttccacggtt tcttcctttt tgctgtctgg gttcagccac
     4021 accagatcaa ccttctggcc cacaaatttc tccagcagtt tttgctggct gaccaggtcg
     4081 tactcgaaat tctgttccag aaccctcaag ctgccggctt cagccaaaga cttaaggttg
     4141 accgtggccg gatcgattct tccaggtaca tcggtaaatc tcagggtctg gattccctgg
     4201 ggcagattaa taaggcgggt ctctttcact acccccacgt ttgaattata aatcgtgaga
     4261 gccacacttt gctggtcctt gagggtactg gtctcagctc ctgccgctcc cggggcccac
     4321 acaaaaaata agacccagag ggaaatcaag ggaatgatcc ttctagatgc gaagatcatg
     4381 ataacctcct ttttctccgg tggcccggcc cagagcctca aggttctttc tttttaaacc
     4441 aaatgtctcc gtaataggcg acggccgact caccggtgtt gtccgtatcg gtcataatcg
     4501 ccactccgtt aatcaagggg ggatcctccc cgaaggcttt ccgataatct tcgaggacgt
     4561 tcctctcttc ggtcacccac tggttgagct tctcggctcc gctctccacc acgatcatca
     4621 tgacccggtc ggtaaaaggg ttgggaacca gggttcctgg gggggcttgg ctctcccaga
     4681 tatagttgag ggcggccaag ggcggatatt ggccgtagaa agctttgacc aggccgtatt
     4741 tggttcgatc cagaaaagac agcttttggg ggtcatattc aaacgtaata taaatacgag
     4801 cgggataatc atctccttct ttgcggcgca catcgccctt tttcaggacg ttctccactt
     4861 tccagcgcca ttggaggatg gggaactctc gggggtcgat cttgatttcc ctaattaggc
     4921 cggaagccga ggcctgggat tcggctttca ccacgaccac attctggtcc ttgaccaggg
     4981 aataaagcgt gtgtttgtcg atattcttga aggtcagggg tttccacccg gcgggaaaac
     5041 tgcctccggc ttcggcggcc gagaacttcc ctgcttcaag gattagaccc tgctgggggc
     5101 tggcaggaca aagcaaaata aaggtaccca cgccaaaggc tgccaagccc ccaagagaga
     5161 ttggcaaggc tctcatattc cctctttgaa ttcaaccgaa tgtcctattg taaccgagac
     5221 gaataaaaag gggaagacgc cgggatggag ctttttcgcc gcccttccag gggtgctgcc
     5281 tgaaaccctg attttagttg acgatcaagc gtgacaggac ccagctgatg aaactgatga
     5341 gcagggaacc gaaaaaggac ccccagaaac cattcacctg gaagcctccg accaggcccg
     5401 ataccatcca aagaaggagg gcgttgatca ccaggaggaa gagccccaga gtgaggatcg
     5461 tgaggggaag agtaagcagg acaaagatgg gtcggacaat ggcattgacg atccccagga
     5521 gaatcgcagc acccacggcg gcccagattg tttccacccg cattaagctg gggaaaaggt
     5581 aagcaatggc caggatagcc accatgttga tcaacaatcg aattataagc ttcataagcg
     5641 gttcctatct aaaaaaaggg atgactgcaa agcgttgata cccgagcagg cgatcggtgc
     5701 ctttcctcgc gctcctccgc acggcatatt ttctgatgcc ctccggcttc tatttcagat
     5761 aaaatgcata tcataaccag ccaggggaag gcaatggcga ttccgcggca gggcagag
//
LOCUS       DASPRR010000557         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2386169, whole genome shotgun sequence.
ACCESSION   DASPRR010000557 DASPRR010000000
VERSION     DASPRR010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386169"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..1502
                     /locus_tag="VEL68_20505"
     CDS             405..1502
                     /locus_tag="VEL68_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38421.1"
                     /translation="MIDDLLQKIELSDKSGVAELIGTEIVSQGRAWPIHLSLYPVAQR
                     VLNPPFINPHLPKMYRVCREFIPYLSEEDIPLLVRLEISEYTRRPKLEKVFPPAQTSS
                     SGPFEDLEAAIREGKREKTAALMAAFQEQRGKAELARRLLLLGSGYLDQTLGHSLSCT
                     AFILLEMMERPEQDSWPALVTLADYFCKGGFSSTPRLRNSALESEQALDHHLLRATTG
                     RGIVNLHHTITRYAVERVRHLLSDAEYSHLISCWIDFLGGKDAASPAIEPSIKINDYP
                     EFYRFFSKREERPVLASFAGLVPTEEGRKRLARYLVRAVCDSYQGNYDPHYLTGLGSA
                     LWVVNRHWNQASLALNALRQYLNYFFTHMIY"
     gene            1785..2639
                     /locus_tag="VEL68_20510"
     CDS             1785..2639
                     /locus_tag="VEL68_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXZ38422.1"
                     /translation="MNGKAEGMRFTGASKYVLDPELARIVNISMALEMPLLLKGEPGT
                     GKTMLAYAIAEALDMPLIVLNVKSSMKLVDALYQYDTLTRLNDSRFGDSKRDVSNIEE
                     YIKMGKIGQAFVSAIRVVLLIDEVDKADTDFQDDMLDVLDQMSFDIVEVDKTIKAKNR
                     PVIVITSNAKKDLSDPFLGRCNFHHIAFPAPDLMREIIVVHFPGLEQDLSKACIDTFY
                     RLREIPGIEKKPATRELINWIRALRCDPDFKPARLNKGEVPYLGVLYKKSMDFTVATQ
                     HLARGIRS"
     gene            2848..4041
                     /locus_tag="VEL68_20515"
     CDS             2848..4041
                     /locus_tag="VEL68_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38423.1"
                     /translation="MFVNFFYKLREVGIPATPTSFLRLHKAMSLGLITSLEDFYTSAR
                     AILVKSERYFDLYDQVFAHYFKGMDLPDQEGIDLDEAARMLLEEWLRDPEGVAQALGV
                     NPEELLNLSPQELIEYFLERLKDQTEAHHGGNKWIGTRGTSPVGHSGHHPGGMRVGGA
                     SMNKSAVKVAMDRRYRDYSMQGLLTQSQIGEALKRLRHMVPFGPKDRVNIDETIYETM
                     RNAGEIEIVFDRSLRDRLKVVLMIDNGGWSMDPYVDVVQVLFDYSRNQFKDLKTYYFH
                     NTIYDYVWADPPRRRKPQKVEEFARLDPETRLVILGDASMAPYELMATDGSIHIEERN
                     LIPSIDRLRFLAKNFRHTAWLNPRVEMEWHYVRTLQTIRMIFPMFELTLDGLEKAVRH
                     LMKNN"
BASE COUNT         1020 a         1257 c         1105 g          993 t
ORIGIN      
        1 atacacggga tcggaaatcc ttctcttctt cactcagcag ggaatcgatc tggtagaaat
       61 ccacgggtcc ccctttcatc atctttcagc ctcgtgcttc cttccttttt tctgctccca
      121 aaactctggt ttttgaacct cgcgcacctt gagcctagcc ggttgtttgc cccttccgct
      181 gacaaccctc cctttcatga ctcttccatt ccttttatta ttataagcgc aatttattcg
      241 cttttatctc tctctttcct ctgctttccc ttatctttcc tggaaaggag cactcttttt
      301 cactgggtca tccgaaaagc agaccatggt aaaatgaaaa agtagaacct ttgaccgttc
      361 gagggagctc ggggaatgaa tggacagcca agagggggtg gaccttgatc gatgatcttc
      421 tgcagaaaat cgaactttcg gataagtccg gtgtcgcgga actgatcggc accgaaatag
      481 tctctcaagg tcgggcatgg cccattcacc tatccctgta ccccgttgcc cagcgggtct
      541 tgaacccgcc cttcatcaac ccgcatctcc ccaagatgta ccgggtctgc cgggaattca
      601 tcccctatct ttcggaagaa gacattcctc tcctggttcg gctagagata tccgagtata
      661 cccggaggcc caaattggag aaggtctttc cacccgccca gacatcctcc tcgggtccct
      721 tcgaggatct cgaagcggcc atccgcgagg gaaagcggga aaagaccgcc gccctgatgg
      781 ccgctttcca ggagcaacgg ggaaaagcag aattggcccg caggcttctg ctcctgggaa
      841 gcggatacct ggaccagacc ctcgggcact ccttatcctg cacggccttc atccttctgg
      901 agatgatgga acgacccgaa caggactcct ggcctgctct agtaaccctg gcggattatt
      961 tctgcaaagg aggattctcc tccacacccc gcctgcggaa ttcagccctg gagtcggagc
     1021 aggccctgga ccaccatctc ctcagggcca ccactgggcg cggtattgtg aatctgcacc
     1081 acacgatcac ccgttatgcg gtggagcggg tacgccatct cctgagcgat gctgaatact
     1141 cccacctgat ctcctgctgg atcgatttct tgggcgggaa ggatgcggca agccccgcga
     1201 ttgaaccgtc tataaaaatc aacgactatc ctgaatttta tcgttttttt tccaaacggg
     1261 aagaaagacc ggtcctagct tccttcgccg gattggtgcc cacagaagaa ggacggaagc
     1321 ggctggcgcg ttacttagtt cgggcggtgt gcgactcgta ccaggggaat tacgaccccc
     1381 attacctgac cggactcggg tcagccctat gggtggtgaa tcgccattgg aaccaggcct
     1441 ctctggcctt gaatgccctg aggcaatatc taaactactt cttcacccac atgatttatt
     1501 gagcggttcg aattttgcct cgaccctccg caactccttc ccgaaccaag tctcaaccat
     1561 ctccgttccg ggccctccac aattggtgga ggtcttttct ccgtgctcat tgccccttgc
     1621 tctaggctct atgcccttac gtctgtttct cattatcaat caaattccct agattgagcc
     1681 ttctcgccaa agttaaagtt gaacttcctc ggcggagaag gtaaaatgaa ccaatgcatt
     1741 tggaaattct caaatctgct gcggaaaggg gaaggaacag acggatgaac ggaaaagcgg
     1801 aaggaatgcg gttcacgggc gcttccaaat acgtcctcga cccggaactg gctaggatcg
     1861 tcaacatttc catggccctg gaaatgcctc tcctgctgaa aggggagccc ggaactggca
     1921 aaaccatgct ggcctatgcc attgccgagg ccctggatat gcctctcatc gttctcaacg
     1981 tgaagtccag catgaagctg gtggacgccc tttatcagta tgacaccctg acccgactga
     2041 acgacagccg cttcggcgac tccaagcggg acgtgagcaa tattgaagaa tatattaaga
     2101 tgggtaaaat cggccaggcc tttgtttcgg ccatccgggt agttctgctc atcgacgaag
     2161 tcgacaaggc cgacaccgac ttccaggacg acatgctcga cgtcctggac cagatgagct
     2221 tcgacattgt ggaggtcgac aagaccatca aggctaaaaa tcgcccggtc attgtcatca
     2281 ccagcaacgc caagaaggac ctctccgacc ccttcctggg gcgctgcaac ttccaccaca
     2341 tcgcctttcc cgctccggac ctcatgcggg agatcatcgt ggtgcacttc cccggcctgg
     2401 aacaggacct gtcgaaggcc tgtattgata ccttttaccg cttgagggaa atcccgggaa
     2461 tcgagaagaa gccggccacc cgggagctga tcaactggat ccgggcgctg cgctgcgacc
     2521 cggatttcaa accagcaaga ttaaacaaag gcgaagttcc ttatcttgga gtgctctaca
     2581 aaaagagcat ggatttcaca gtagcaactc agcatctggc taggggaatc cgtagctgat
     2641 acctctataa caagagaggg gcgaattggt ttaggcccct cagctgtgat tcagtcaggc
     2701 gaattcgaac cacgaaatcc tcgataaaga agtgtcaatg tgagtgcttg gtgccagtcc
     2761 gaattctttt tactaaacaa tgacgctgac actttccaca gttttcggat tctggtattc
     2821 ggatctcgga tttattgagg ttttccattg tttgtaaact tcttctataa acttcgcgaa
     2881 gtgggcattc ccgcgacgcc tacttctttc ctccgcctcc acaaagccat gagtctgggg
     2941 ctgatcacct cgttggagga tttttatacc tccgcccggg caatcctggt caagagcgaa
     3001 cgctactttg acctctacga ccaggtcttc gctcattatt ttaagggaat ggaccttccg
     3061 gaccaggaag ggatcgacct ggacgaagcg gccaggatgc ttctggagga atggcttcga
     3121 gaccccgaag gggtcgccca ggctttgggt gtgaacccgg aggagctgct gaatttaagc
     3181 ccccaggagc tcatcgaata tttcctagaa cggctgaaag accagaccga agcccaccac
     3241 ggcggcaaca agtggatcgg gacccgcggc acgtctccgg taggccactc cggacaccat
     3301 cccgggggaa tgcgagtcgg tggagcatcc atgaacaaga gcgcggtcaa ggtggccatg
     3361 gaccggcgct accgcgacta ttccatgcag ggcctcctga ctcagtcgca aatcggggag
     3421 gctttgaaac gcctacgcca catggttccc ttcggcccca aggatcgggt caacatcgat
     3481 gaaaccattt acgagaccat gcgtaacgcg ggggagatcg agattgtttt cgaccggagc
     3541 ttgagagacc gtctcaaagt cgtcctgatg atcgacaacg gcggctggtc catggatcct
     3601 tacgtggacg tggttcaggt tctgttcgat tactcccgga accagttcaa ggacctcaag
     3661 acctattact tccataacac catctacgat tatgtctggg ccgatccgcc gcgccgccgc
     3721 aagccccaga aagtggaaga gttcgcccgt ctggaccccg aaacccgcct agttatcttg
     3781 ggggacgcca gcatggcccc ttacgagctc atggccacgg acgggtccat ccatatcgaa
     3841 gagcggaacc tcatcccttc catcgaccgc ctgcggtttt tggccaagaa cttccgccac
     3901 accgcctggc tcaacccgcg ggtggagatg gagtggcact acgtccgcac cctccaaacc
     3961 atccgcatga tctttcccat gttcgagctc accctggacg gcctggaaaa agccgtccgg
     4021 cacctcatga aaaacaacta atctctcagc tacatgaaag gttgaaccga atcgaagagg
     4081 gacggatcgg ttcgaatcga ctgattccct ccctcccgca accggattgc ggccaaaggc
     4141 tcgagatcgg ggatgggaga atcgatgtac tcaggaaata aggcaacccg gcggccaagg
     4201 aaaaaagaga gatttacgcg tcaccatctc ctcgacccag gaggaaaatg tctttgccga
     4261 ccgagtttta caagtggata ttgaagaatg gaattacaac ttcggaccga cacattttct
     4321 attgttcatc agaattcaaa gcggcagggt taccaatacg gaattcggtg attgg
//
LOCUS       DASPRR010000558        13205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1102139, whole genome shotgun sequence.
ACCESSION   DASPRR010000558 DASPRR010000000
VERSION     DASPRR010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13205)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13205
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102139"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1508)
                     /locus_tag="VEL68_20520"
     CDS             complement(111..1508)
                     /locus_tag="VEL68_20520"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXZ38424.1"
                     /translation="MMEFIYGKILYLWTRKAGCQDVLSKWGRAHSGFLVLFFLFPLLN
                     PFPSQSQETPQSWNIHGQTTAITQAHGGFHSPYEGPNSFRSKWELHTVSVSTLFLGKR
                     LWPGGEFYVNPELAMGRGLSDVLGIAGFPNGEATRVTKENPTLYLARLFLRQTWNLGG
                     GEEVITDSPNFLAGKRSKRRLALTAGNFAATDLFDDNLYSPHDPSTHFFNWGLMNAGA
                     WDFPADTRGYTWGFALDFTWDRWSARWGSFLVSTSANGLNFDMQITQARGDVAEIEHR
                     HLWGEREGAVRFLLFMNQAFMGSYRESLNLKPQGPNIEDTRQPGRKKYGWTISAQQEV
                     IRDLGTFLRVGWNDGKTESWMFTEIDRSLAWGVSLGGRSWDRPLDRLGLAIMANGLSQ
                     DHREYLAAGGLGFMLGDGRLNYAPEGIVEGYYAFTFFNEATNISLDVQRVWNPGYNHD
                     RGPVTIFGLRLNLAI"
     gene            complement(2119..2349)
                     /locus_tag="VEL68_20525"
     CDS             complement(2119..2349)
                     /locus_tag="VEL68_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38425.1"
                     /translation="MTPFFRIKHYLINLDRLAYVRIEENFIDFGFSFPVEKSSGHHFI
                     RLERGSNLNPAEFDQVKEFVLQLPDPDRVIVV"
     gene            complement(2374..5436)
                     /locus_tag="VEL68_20530"
     CDS             complement(2374..5436)
                     /locus_tag="VEL68_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXZ38426.1"
                     /translation="MRRWIIFVTEKNIVFLLLGSCAFLISLFLVKDLNVEAFPDPSAP
                     QIEAVVIYEGKSAEEVEKRITLPMEVGLASMRGLQRLTTTSLYGLCDIKCKFSYDIPY
                     REARQEVINRFSNIVLPDGAQPTIIASDMGEVMQYVLVGSNNLMELRTLQEWVAARYI
                     KTAQGVQDVASYGGFIKAYVVTVRPEDLIKYGITLSQVIEGLSKSNINVGGRVVELGD
                     QYYMVRGLGLIRSLPDIENSVIAYKGGKAILIKNVAQVKLGNIPRTGIIIFNKNDDAV
                     MGNVIIRRGEKSIPTIRSINEKVADLNGKILPKGIKAVPYYERWGLITTVIKKVVETA
                     SSGVFLVAVGLFLFLGNLRAAIMTALVIPFSLAITLAVMAMRGDSANLLSIGAIDFGI
                     IADVALVLTENYIRVARQYALGRGPLTRTTGERALIKAAGEVGTPIILLVLIILLAFI
                     PIFTMKGAEKQIFSPMAETYCYVLLFTLILTLTFLAASIHTFLEGHEGTEFRFFEILQ
                     QSYLKLISLLLRKPRIVILLTSLILLTGFGVGFKVIGTQFLPKLDEGNIYIRITFPYS
                     ISLTKTHENAKRVRDILIELPEAKSVAVRIGRPEDGTEATGPSNTEYYVNLHPYNKWQ
                     RKITKEQLEEEVRQKLTQLFPRVNISLSQYMEDNLNEVTSGVKGENVVKVFGENLQEL
                     DRIAQEIEKKIGKVPGIEGVGLFKELGQPNLLIEVDRENASAVGLTVQEVLDMVSAAL
                     GGKVVSQVVEGDKNFALQVSFPYDYRREPDKIGKIPVVLPTGGIIPLARVAKIRYDTG
                     ASFIFRENHKRLIPVKFSVVSKDLGGTVMKAQKETEQIKLPEGYFLDWAGQFNEMLEA
                     FRRFYLSIPFAIFLISILLYLFYGNMRNVALTAVAPICSVFGGVVCLLLTGESLSISA
                     VVGFVSVIGISIFNTCIWITHYIEIYREKKDEEKATLETTQDKFRPVLMGGLIASLGL
                     FPASIAHGVGSQVQKPLALVVVGGMFIGTLIILLVMPLLFKFVKVGKK"
     gene            complement(5433..6482)
                     /locus_tag="VEL68_20535"
     CDS             complement(5433..6482)
                     /locus_tag="VEL68_20535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ38427.1"
                     /translation="MKRLMMFLVSILLFSGCTGDPEPKEKAPGMERKIETYRVSCQEI
                     PSSIEATGTIQPDIEGSAKIVSGLAGTVENIFVKVGDRVRKGDPLCAIRSPDVSDTYS
                     NYLANLSQVKQAERIFNLNKQLFEVGAVTKNDLLNSEANYEQTKAQSEALKKKLEIYG
                     VKPESGFSDRYTIHAPLEGAVVEITAHVGDRVDTTNGLMTIADPSKVVVVANIYDTDI
                     AHVQKGKTVTFYTDISPNTPFKGLIKYISDTSDPDTKTVKTYIRIVSGENPFRQNMFL
                     KIKITNAKKKLPVVPKTALLYKDGKFSVYLRVGGKDEVKEVKPVLDAGEKFMAVEGLK
                     EGDEVVLSAIEMEKT"
     gene            complement(6479..7639)
                     /locus_tag="VEL68_20540"
     CDS             complement(6479..7639)
                     /locus_tag="VEL68_20540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXZ38428.1"
                     /translation="MTLDQALALFYQNNYDLLIHRYEMDKAYADYVGAKLRPNPTLSF
                     NANGLDYYSGYLKRDENTQITVRIDQLIELGGKRELRTNAALAVQQAATLTHKDVIRN
                     LLLGFYTVYYNLELDRLNMEFGRDELTRFDRILDIAERRFNAGFLSQLDYTKIKLAKI
                     DLENNLTNSQAQFRKDLENFNVLLGAVTTLEPSRLGIREEFPAYQEESLIEVAYQNRY
                     DLLAAQKQVEAARHNMALARALRIPDLSVGAEHDSFGPDGASRIGGGVSIGIPVFSRA
                     QGAILKSNAEFKQLEEQVRKIKRVIVSDVRQGLISYRASLAILGSYKERKASMEDLLN
                     KSEAAFSLGGITVLDLLDTRKTYRDFVTKYNQNLIQTLLNQELLKVFTGEVK"
     gene            complement(8400..9107)
                     /locus_tag="VEL68_20545"
     CDS             complement(8400..9107)
                     /locus_tag="VEL68_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38429.1"
                     /translation="MLTLGQIDVYYGNIQALWKVSLGVEEGEIITIVGSNGAGKSTIL
                     RSITGLIKPRRGSITFDSRRIDALSPDEIVRRGVSMVPEGRELFPRMTVLENLELGAA
                     YIDRAYGQTAGSRQWVLALFPVLQERSKQQAGTLSGGEQQMLAIGRALMSRPKLLMLD
                     EPSLGLAPLLVAGVFRTVQQINQEGVTVLLVEQNVRQSLTLAHRAYVLENGRMVMEGE
                     GKDLIADKHVKEAYLGL"
     gene            complement(9101..9850)
                     /locus_tag="VEL68_20550"
     CDS             complement(9101..9850)
                     /locus_tag="VEL68_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38430.1"
                     /translation="MSLLEVSGLTKHFGGLAALQKVSFAIDRGEIVSLIGPNGAGKTT
                     IFNLFSGVYPPDAGEIRLDGDDLVGHRPFEICRRGVGRTFQIMQPFPHMTVLENVITS
                     GLFGRVPRVSMKRVEEEAMELCRWVGLGEKMGVYPRNLPVADQKRIEIARALAIKPKL
                     LLLDEVMSGLTPTETTEAIKLVRQLRERGITIFLIEHVMKVVMGISDRVIVLHHGEKI
                     AEGPPQRVAHDQKVIEAYLGEQIDNSEESSC"
     gene            complement(9923..10861)
                     /locus_tag="VEL68_20555"
     CDS             complement(9923..10861)
                     /locus_tag="VEL68_20555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38431.1"
                     /translation="MNRSFSWIKTLFPAFLILLALWFPRLVDAYFLHLAVTFFLAMIL
                     ASSWDILARTGQVSVGQAGLFGIGAYTAALLFKWIHLSPFLGMLAGAVISLIVAALLG
                     YLTLRLRGIYFSITTIAFAEALSVIWLMTPGVTGGAMGLSTPPLFGGDRQQAYYLILG
                     ILIFCLLIVWMIEATRLNFAFTAIRENEEVANVMGINPTRYKVLAFMISAFFTGFAGG
                     FYTHYNTYIIPYEVFGLGISIACLVMPIFGGLYTITGPVIGTVIVKVIEEYLRVTVSY
                     GHQIAYGLILVLVVLFMPDGVMGIWRNKILPLFKAR"
     gene            complement(10867..11745)
                     /locus_tag="VEL68_20560"
     CDS             complement(10867..11745)
                     /locus_tag="VEL68_20560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38432.1"
                     /translation="MEWADFLQSFANGLLIAGLYAAVTLGLTLVLGVMGIVNFAHGEM
                     VMLGAYNTFWVFTLLGIDPLLSIALSGLLLFFIGLFIYRFTIRPILNDPPLNQLLLTL
                     GISIFLQNLAMILWKTDSRSVITSYSGMSLSLGVVNVGMTRLLTFLIALGLTLFLLFF
                     LYKARPGKAMRAVSENNTASWLIGINVQKTYLLAFGVACALAGASGALVSTVMYTYPM
                     VGFKLSLKAFCILVLGGLGNIPGALFGSLILGLTESFVGTYVPEGSGWAEGISFILIM
                     VILIIKPTGLVGTRRE"
     gene            complement(11861..13036)
                     /locus_tag="VEL68_20565"
     CDS             complement(11861..13036)
                     /locus_tag="VEL68_20565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ38433.1"
                     /translation="MILFIVAVQAGTAIAQDTIKIGAVLPLSKAAFAQAGEEQRRGLL
                     MALEEVNQAGGVLGKKVELTIEDDTGEPSVGIAAAEKLIARDKVVALIGGYSSTITYA
                     QLNAIQRYEPFVAWVGASSTKVEHEFGPKRWFFHYHPWDYHRQSTVRDFLLSISPQPK
                     AVAVAYEDGIYGTTSRDYVVKYLKEKGFNVVFDERFKSGSSDFTPMLTKIKRTNPEVF
                     YWVAYAGDTTLIMKQAKEIDFNPKLFLSVAVNFPQYKPSLGATGDYVSGVDVWVPGMK
                     LPETVKWMEKFKKAYPDRTPEYWVPLAYTNLMTVVEAIKGAGSTDKEKLIAAMEKTDY
                     NSPMGKMTFKKSEEGGLHQAIDEQIVTQWQAGVSHVVYPASKATSKLIYPTPNWKSR"
     gene            complement(12978..>13205)
                     /locus_tag="VEL68_20570"
     CDS             complement(12978..>13205)
                     /locus_tag="VEL68_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38434.1"
                     /translation="LLVSLEICGNNLPPFSFRETARADNAKDAKMDHKIFSRRFSNGR
                     KETIMVLGASGIFDSFYRRRTGGNRDRPGHD"
BASE COUNT         3122 a         3604 c         3405 g         3074 t
ORIGIN      
        1 tggcttacta cgaaacgctg actgagtacg ccaaagcccg ggttgcgttg gagcaagcgg
       61 cgggcatatg ggaggtgaat ttttaattgt tcatcctgca ttaccctcgt ttaaatagcc
      121 aggttcagcc ggaggccaaa aatcgtcacg ggtccccggt catgattgta gccggggttc
      181 cagactcgtt ggacatccaa agagatgttg gtggcttcgt tgaaaaaggt gaaagcgtaa
      241 tacccttcca cgattccttc cggggcgtaa ttgagccgcc cgtctcccag cataaacccc
      301 aagccgccgg cggccaggta ctcgcgatga tcctggctta agccgttcgc catgatggcc
      361 aagccgagtc ggtccagggg tcggtcccac gaccgccccc cgagggaaac cccccaggcc
      421 aaactacggt cgatttccgt gaacatccag ctttccgtct ttccatcgtt ccagccgacc
      481 cgcagaaagg tgcctaaatc ccggatcact tcctgctgcg cgctgatggt ccacccgtat
      541 ttcttccggc cgggctgccg cgtatcctcg atattagggc cctgcggctt gaggttcaag
      601 ctctcccggt agctacccat aaaggcctga ttcataaaaa gaagaaagcg gaccgcgccc
      661 tcccgctccc cccaaaggtg gcggtgctcg atttcggcta cgtctccacg ggcctgagtg
      721 atttgcatgt caaaattgag gccgttggcc gaagtggaaa ccagaaaact cccccagcgg
      781 gcactccagc ggtcccaggt aaaatccagg gcgaaccccc aagtatagcc tctcgtatcc
      841 gcggggaaat cccaggcccc tgcgttcatc agtccccagt taaagaaatg ggtgctcggg
      901 tcatgggggg aatagagatt gtcatcgaaa aggtccgtgg cggcgaaatt tcccgccgtg
      961 agagccaggc ggcgcttgga cctcttaccc gccaaaaaat tggggctatc ggtgatcact
     1021 tcttctccgc ctccaagatt ccaggtctgg cgcaggaaca accgggccag gtaaagggtg
     1081 gggttttcct tggttactcg ggtcgcctca ccgtttggaa aaccggcaat gcccaggaca
     1141 tcgctaagcc cccgacccat tgcgagctcc ggattcacgt aaaattctcc gcccggccaa
     1201 agccgcttgc ccaggaaaag ggtggagacg ctgaccgtat gcagctccca cttagatcga
     1261 aaactattgg gtccctcgta gggggagtga aatcctccgt gggcctgggt aatagccgtc
     1321 gtttgaccgt gaatattcca agactgaggg gtttcttgcg actgagaagg gaaagggttc
     1381 agcaaaggga aaaggaaaaa aaggaccaga aaaccgctat gcgccctccc ccacttgcta
     1441 agcacatcct ggcaacccgc cttcctcgtc cacagatata agattttgcc ataaatgaat
     1501 tccatcataa ctctgccccg ggattttccc tctccattct taaacctttt gccatatgaa
     1561 tgcttggggc cgggatagac atccaccgtt ctatctcaaa ataaggaatc gctccgaggc
     1621 cgatattacc gcgagcaggg tttcggattc aacccatttc ctctacccat cacgcgaaaa
     1681 ataatctact tttccgggtt cacccagatg atttccacga agaagttcac cgatgacccg
     1741 agaaaacacg ggtccaacaa cgcaggggga ggggattttt cgaatgagaa aagaatcgga
     1801 aatttccggg gcgaggaaac gcctggcggg gccttctccg aggggaagat catgccagga
     1861 tcactcgcct tcatggaatc tcattttcct ttcgagctgg acagaaaccc atccgaaaaa
     1921 ggattctcca gatgcgggct ggtgcataaa aaatcagcct cggccaaatt ataaaaatgg
     1981 acataggggg aaaaaaggcc cagggggaga aaatggcagc gaagaggact ttgcctctct
     2041 gccttcccgg cctcataatc caatcatgcc ttgagagcga gtacagaata tgccggcgcg
     2101 ggtctcatgc ccccaacgtt ataccacaat cactctatcc gggtcgggga gttgcagcac
     2161 aaattctttc acctggtcga attctgcagg gttcaaatta gaacctcttt ctaggcggat
     2221 gaaatggtgt ccgctgcttt tctccacggg aaaagaaaac ccaaaatcga tgaagttctc
     2281 ctctatccgc acataggcca accgatctaa gttaataagg taatgcttga ttcgaaagaa
     2341 aggggtcatc gagcacctcc gttgggaatt cctttatttt ttccccacct tgacaaactt
     2401 gaagagcagc ggcatcacca ggagaatgat gagagtcccg ataaacattc ctcccaccac
     2461 tacaagggcc agcggtttct ggacttgact gcccactcca tgggcgatgg aggcgggaaa
     2521 gagcccgaga gaagcgatga gcccccccat aagaaccggg cggaatttat cctgggtcgt
     2581 ttcgagggtt gctttttctt cgtctttttt ctcccggtag atttctatgt agtgcgtaat
     2641 ccagatacag gtgttaaaga ttgaaatccc tatgacagat acaaatccga ctacggctga
     2701 gatgctaaga gattcgccgg tcaatagcag gcagacaact cccccaaaaa ccgaacaaat
     2761 tggggcaact gccgtcaggg ccacattgcg catatttcca tagaagaggt aaaggagaat
     2821 ggagataagg aaaatggcaa acggaataga caggtagaac ctgcgaaaag cctccaacat
     2881 ctcgttgaat tggccagccc aatctaaaaa gtatccttcc ggaagtttga tctgttccgt
     2941 ttccttctgg gccttcatga ccgttccacc caggtccttg gagaccacgc tgaattttac
     3001 ggggattaac ctcttgtgat tttcccggaa aataaaggag gctcccgtat cataacggat
     3061 cttggctacc cgggcgaggg gaataatccc gccggtcgga aggaccacag gaatttttcc
     3121 gatcttgtcc ggctccctcc ggtaatcgta gggaaagctc acctgcaggg caaaattttt
     3181 atccccctct actacctggc ttactacttt accccccaaa gcagcagaaa ccatatccaa
     3241 aacttcctgg acggtgaggc ccactgcgga ggcgttttcc cggtccactt cgatcagaag
     3301 gttgggctgg ccaagttctt taaaaagccc caccccttca attcccggaa cctttccaat
     3361 tttcttttct atttcttggg caatgcggtc gagttcttgc aggttctcac caaaaacctt
     3421 gaccacgttt tcccccttga ccccggaggt cacctcgtta agattatctt ccatatattg
     3481 ggataagctg atattcactc tggggaaaag ctgagtcagc ttttgtctta cctcttcctc
     3541 caattgctcc ttcgtgatct tccgctgcca tttattgtat gggtgcaggt ttacataata
     3601 ttccgtgttc gaagggcccg tggcctcagt gccatcttcc ggccttccaa ttcggactgc
     3661 gacggacttc gcctcgggca gctcaatgag aatgtcccga accctcttgg cattttcatg
     3721 agtcttggtc agcgatatgg agtaaggaaa ggtgattcgg atataaatat tcccctcatc
     3781 cagcttggga agaaactggg ttccaatgac cttaaaacca acaccgaacc cagtgagaag
     3841 gatgagagag gtaagaagaa tcactatacg cggctttcgg aggagcaaag aaatcagctt
     3901 caaataggat tgctgcagga tctcgaaaaa tctaaactcc gttccctcgt gcccctccag
     3961 aaacgtgtga atggaggccg cgagaaaagt gagggttagg atgagggtaa agaggagaac
     4021 atagcaatag gtctcggcca taggagaaaa aatttgtttt tctgcccctt tcatggtgaa
     4081 gatgggaata aaagcgagga ggatgatgag taccagaaga atgatcggtg tccccacctc
     4141 ccccgcagcc tttattaaag ccctttctcc cgtggtcctg gtcagaggtc ctctcccgag
     4201 cgcatactgt cgagccacgc gtatgtaatt ctcggtcaag accagggcaa catcggcaat
     4261 gatgccgaaa tcaatggccc cgatggatag caagttggcg gagtctcccc gcatagccat
     4321 gaccgcaagg gtaatcgcca gcgaaaaggg gatcaccagg gctgtcataa tggccgctct
     4381 caagttgccc aggaagagaa aaaggccgac ggcaacgagg aacaccccgg aggaggcggt
     4441 ttccactacc ttcttaatca cggtggtgat gagcccccac cgctcataat aaggtactgc
     4501 ttttatccct ttgggaagga ttttgccgtt caggtccgct actttttcat taatagatcg
     4561 gatggtaggg atggacttct cccctctgcg gatgatcaca tttcccatga ctgcatcatc
     4621 atttttattg aaaataataa ttccagtccg gggaatgttc cccagcttca cttgggctac
     4681 gttctttatg agaatcgctt ttccgccctt ataagctatc acgctgttct ctatgtctgg
     4741 cagactcctg atgagtccga gtccccttac catgtaatac tgatctccca attcaacaac
     4801 tcttcctccc acattgatat tagacttgga gaggccttca atcacttggg agagggtgat
     4861 cccatactta atgaggtcct cgggacgaac ggttaccaca taagccttta taaagccacc
     4921 gtagctggct acatcctgga caccctgggc ggtcttgata tacctggcgg caacccattc
     4981 ctggagagtc cgcagttcca ttaaattatt ggaaccgacc agaacgtact gcattacctc
     5041 ccccatatca ctggcaatta tggttggttg ggcgccatca ggcaatacaa tattgctgaa
     5101 ccgattaatc acctcctgtc tggcttctcg ataaggaatg tcataagaaa atttgcattt
     5161 gatgtcgcag agcccgtaaa gggatgttgt agtcaacctt tgcagcccgc gcatactggc
     5221 taggccaact tccatgggga gggtaattcg tttttccacc tcttcggcgg attttccttc
     5281 gtagatgaca acggcctcaa tttggggggc ggaaggatcc gggaaggcct cgacgttgag
     5341 atccttgacc aaaaaaaggg agatcagaaa ggcgcatgat ccaaggagca aaaaaactat
     5401 atttttttcg gttacaaaaa taatccatct tctcatgttt tttccatctc aatggccgat
     5461 aatacgactt catccccttc ttttaaaccc tccacggcca taaatttctc tcccgcatct
     5521 agtaccggct taacctcctt tacctcatcc ttacctccca ccctcaagta aacactgaat
     5581 tttccatctt tgtacaaaag ggcagtcttg ggcactacgg gaagtttctt tttcgcattg
     5641 gtaattttta tctttaaaaa catattttgg cgaaacgggt tctccccgct tacgatccgg
     5701 atgtaggttt taacggtttt tgtgtccggg tccgaggtat cactgatata tttgatcagt
     5761 cctttgaaag gggtgttggg ggaaatgtcg gtgtaaaaag tcaccgtctt tcccttttgc
     5821 acatgggcga tatcggtgtc gtatatgttg gccacgacca caactttact cgggtcggcg
     5881 atcgtcatta aaccgtttgt cgtatctacc cggtccccga catgcgcggt gatctctacg
     5941 acggccccct ccaggggcgc atggatggtg tagcggtcgg agaagccgga ttccggcttc
     6001 actccgtata tttccagttt tttcttcaag gcttcggact gtgccttggt ctgttcataa
     6061 ttggcttcgc tgttaaggag gtcattcttg gttaccgccc ccacttcgaa aagctgcttg
     6121 ttcaggttga agattctttc cgcctgtttc acctgcgaaa ggttcgccag gtaattggag
     6181 tacgtatcgc tgacgtccgg gctccggata gcgcacaggg ggtctccttt tcttacccgg
     6241 tcaccgacct taacaaaaat attctccacg gtcccggcaa ggccggagac gattttggcg
     6301 cttccttcga tatccggttg aatggtgccg gtggcctcga tggaactggg gatttcttga
     6361 caagacaccc gataagtctc gatctttctt tccattcccg gcgccttctc cttcggttcc
     6421 gggtcccccg tgcatcccga gaaaagcagt atggagacga ggaacatcat cagcctcttc
     6481 attttacttc tcctgtaaag actttcagaa gctcctggtt caagagcgtc tggataagat
     6541 tctggttata tttggtgaca aaatcgcgat aggtcttccg cgtatccagc aagtccagaa
     6601 ccgtaatgcc ccccagcgaa aaagccgctt cgctcttatt gagcaggtct tccatggatg
     6661 ctttccgttc cttataggag cccaagatcg ccagggatgc ccggtaactg atcagcccct
     6721 gtctcacatc ggatacgatc accctcttga tttttctcac ttgttcttcg agctgtttga
     6781 attccgcgtt gcttttcagg atcgccccct gagctcgact gaacactgga atcccgatgc
     6841 tgactccccc gccgatccgg gaggctccat ccggtccaaa cgaatcgtgc tccgctccca
     6901 ccgagaggtc cggtatccgc aaggccctgg ccagggccat attgtggcgt gccgcctcga
     6961 cctgcttttg agcggcgaga aggtcgtatc ggttttggta ggccacctcg atcaggctct
     7021 cttcctgata ggccgggaat tcttcccgga tgcctaacct cgagggttcg agcgtcgtga
     7081 ctgccccgag gaggacgttg aagttctcca ggtctttccg gaactgggct tgagaattgg
     7141 ttagattatt ctccaggtct attttggcca atttgatctt cgtataatcc agctgggaca
     7201 agaaccccgc gttgaaccga cgctcggcga tatccaagat ccggtcaaac cgggtcaatt
     7261 catccctgcc gaattccatg tttaaccggt ccaattcgag gttgtaatac accgtataaa
     7321 atccgaggag caggttccgg atgacgtctt tatgggtaag cgtagccgct tgttgaacgg
     7381 ccaaggcggc gttcgtcctc agttcccttt tcccgcccag ttcgatcagt tggtcgatcc
     7441 gcacggttat ttgcgtgttt tcgtcccgct tcaggtatcc gctgtaatag tcaagtccgt
     7501 tagcattgaa cgacagggtc gggttcggcc tcagcttggc tcccacataa tccgcatagg
     7561 ccttatccat ctcataccga tggatcaaaa ggtcataatt attctgataa aagagagcca
     7621 gagcctgatc caaagtcacc tcttcccctc tccccaaagt gggtggggaa aagaataagg
     7681 cccccaggta aaggaacaaa ataagcctga acctatttac cagcctggaa tggtaaggcc
     7741 cattcccaaa ttcacctttt ggaatgtatc tccgaaacat cctttccata gttggcctag
     7801 ccccgaagag cttatctttt ccctgccccg aagcagcccg gggaacggta ggttttggtg
     7861 atgaacgccc tattgttata accagactga aagagcgcgt aaatacacag gaatgaatcg
     7921 ccggcattcg ctgagggatc gagaggagat caacaccgaa gattgggcgg gaaagattcc
     7981 caatggcaga gaatggatga gaagctcttg agctccgaga aaagtgtttc gtctaaaaag
     8041 aaaaaagcct gtaatgaggt gggtttgagg tctaaaatat tctgtttgtc gacgtaaaga
     8101 tcatctaagt cgcttgactc aaattttaat cccttgtata aaaaatctgc ttcgatcaag
     8161 acgtagtaat ggaagtaagt agaagaggcg agcaaaatca gggagcccgt gatgataaat
     8221 agcaaaaggc ccttgggttt tttcatcgtg tgaaagctcg atgatacttt ctttattgaa
     8281 atcaccaacc gatcattagc ttaatatttt tttatccttt tttcctcgta attgcaagga
     8341 aaaatttcag ctccttctgt ccacccgatc acctgaatac cttgaattct taagaaattt
     8401 caaagtccta gatacgcttc cttcacatgc ttgtcggcga tcaggtcttt cccctccccc
     8461 tccatgacca tccgcccgtt ctccaggacg taggcccggt gcgccaaggt caggctttgg
     8521 cggacattct gctccaccag aagaacggtc acgccttcct ggttgatctg ctgcaccgtg
     8581 cgaaaaacgc cggctaccag caggggggca aggcccaggg acggctcgtc gagcatgagg
     8641 agcttgggcc gcgacatgag ggcccggccg atggccagca tctgctgttc cccaccgctc
     8701 agcgttcccg cctgctgttt ggaacgctcc tggagaaccg gaaagagggc gaggacccac
     8761 tgccgggatc ccgcggtctg gccgtaggcc cgatcgatgt aggctgctcc caactccagg
     8821 ttttccagaa ccgtcatccg gggaaagagc tcccgccctt ccgggaccat ggataccccc
     8881 cggcgtacga tttcatccgg gctgagggca tcgattcggc gcgagtcaaa ggtgatggag
     8941 cctctccggg gtttaatcaa tcccgtgatg ctgcgcagga tggtcgattt gccggcgccg
     9001 ttggacccaa cgatggtaat gatctctcct tcctcgacgc cgaggctcac cttccacaga
     9061 gcctggatgt tgccgtaata gacgtcgatc tgaccgagcg ttaacacgat gactcctcgg
     9121 aattatcgat ctgctccccc aggtatgctt cgatcacctt ctggtcatgg gctacccgct
     9181 ggggaggtcc ttccgcaatc ttctccccgt ggtgcagaac aatgacccgg tcggagatcc
     9241 ccatgacaac cttcatcacg tgttcgatca ggaagatggt gatgccccgc tcccgcaact
     9301 ggcggaccag cttgatggct tcggtggttt ccgtgggggt cagcccggac atcacttcat
     9361 ccaggaggag gagcttcggt ttgatggcca gggcgcgggc gatctcgatc cttttttgat
     9421 ccgccaccgg taggtttctc ggatacaccc ccattttttc ccccagaccc acccaccggc
     9481 acagttccat ggcctcctct tccaccctct tcatggaaac cctggggacc cgcccgaata
     9541 ggccgctggt gatcacgttt tcaaggacgg tcatgtgggg gaaaggctgc atgatctgga
     9601 aggttcttcc cacccccctc cggcagatct cgaagggccg gtgacccacc aggtcgtccc
     9661 cgtccagccg gatttccccc gcgtctggag ggtagactcc cgaaaaaaga ttgaagatag
     9721 tggttttgcc ggctccgttg ggaccgatca ggctgacgat ttccccgcga tcaatcgcaa
     9781 agcttacctt ctgaagggct gccagaccgc caaaatgttt ggtcaatccg ctaacttcaa
     9841 gaagcgacat aataaagacc ctaaagagtg agtcgtgagc ccctgtaggg gcgggtttca
     9901 aacccgccta tacaaatccg tctcatctgg ccttaaataa gggcagaatc ttgttccgcc
     9961 agatccccat gaccccgtcg ggcatgaaaa gaaccaccag caccaggatc aagccgtagg
    10021 cgatctggtg gccgtaggaa acagtaaccc ggagatattc ttcaataacc ttgacgatca
    10081 ccgtgccgat gaccggtccg gtaatggtgt acaacccccc gaagatgggc atgaccaggc
    10141 aggcgatgga aatgcccagg ccgaaaactt cataaggaat gatgtaggtg ttgtagtggg
    10201 tgtaaaaccc cccggcgaaa ccggtaaaaa aggcgctgat cataaacgcc aggaccttgt
    10261 accgggtggg attgatgccc atcacgttgg ccacctcttc attctcccgg attgcggtga
    10321 aagcaaaatt gagccgggta gcctcaatca tccagactat taataggcaa aaaatgagaa
    10381 ttcccagaat aaggtagtat gcctgctgtc ggtcccctcc gaagagagga ggggtggaga
    10441 gacccatggc tcccccggtg actccaggag tcatcagcca gatgaccgaa agggcttcgg
    10501 caaaggcgat ggtggtgatg gaaaaataga tcccccggag gcgaagggtc aggtacccta
    10561 gcagagcggc gacgatgagc gagatcactg ctcccgccag catccccaga aacggagaca
    10621 ggtgaatcca tttgaagagg agggccgccg tgtaggctcc gattccaaag agcccggcct
    10681 gccctaccga tacctgtccg gtccgggcca ggatgtccca gctgctggcc aggatcatgg
    10741 ccaggaagaa ggtgacggcc agatggagaa aataggcgtc caccaggcgt ggaaaccaca
    10801 gggccaagag gatcagaaaa gcaggaaata gggtttttat ccaggaaaag gaacggttca
    10861 ttctctttac tccctgcgag tccccaccaa tcccgtcggc tttataatga gaatgaccat
    10921 gatcaggatg aaggaaatcc cttccgccca ccccgacccc tcgggaacat aggtccccac
    10981 aaaggattcg gtgaggccta ggatcaggct gccgaaaaga gcacccggaa tattccccag
    11041 ccctcccaga accaggatgc agaaggcctt caggcttaac ttaaagccca ccatgggata
    11101 ggtatacatg acggtgctga ccagagcccc ggaagcgcct gccagggcgc aggccacccc
    11161 aaaagccagg aggtaggtct tctgcacgtt gattccgatc agccaggagg cggtgttgtt
    11221 ctcgcttacc gcgcgcatgg ccttccccgg ccgggccttg tagagaaaaa agaggagaaa
    11281 gagggtgagc cccagggcga tcaggaaggt gagcaggcgg gtcatcccca cgtttaccac
    11341 tcccagggaa agggacattc ccgagtagga agtaatcacc gaccgagagt cggtcttcca
    11401 caggatcatg gctaggttct ggaggaagat ggaaattccc agggtcagaa ggagctggtt
    11461 caggggagga tcgttgagga tcgggcggat ggtgaagcgg tagataaaga gcccgatgaa
    11521 aaaaaggaga agcccgctca gggcgatgga aaggagggga tcgatcccca gcagggtgaa
    11581 tacccagaag gtgttgtaag cccccagcat gaccatttcg ccgtgagcga agttgacgat
    11641 ccccatcacc ccgagcacca gggtgagccc cagggtgact gccgcataaa gcccggcgat
    11701 cagcagtccg ttggcaaagc tctgcaggaa atcggcccat tccataaaag gctcgcaacc
    11761 gtgcaggggc catcacgcgc ggggtcatga attgctcctg cacctatcaa gggcattcta
    11821 ggccgtaggg gcgggtttga aacccgcccc cagactccag ttatcggctt ttccagttgg
    11881 gggtaggata gatcaatttg ctggtggctt tggaggccgg gtagacgacg tgagaaactc
    11941 ccgcctgcca ttgggtgacg atctgctcgt cgatggcctg atgaagtccc ccttcctcgc
    12001 tcttcttgaa ggtcatcttc cccatgggag agttgtagtc cgtcttctcc atggcggcga
    12061 tgagcttctc tttgtccgtg gacccggccc ccttgatggc ctccaccacg gtcatcaggt
    12121 tggtgtaggc caggggcacc cagtattcgg gggtccggtc ggggtaggcc tttttgaatt
    12181 tctccatcca cttcaccgtc tccggaagct tcattcctgg tacccataca tctactccgg
    12241 agacatagtc cccggtagcc cccagagagg gcttgtactg ggggaaatta accgccaccg
    12301 agaggaacag cttggggttg aagtcgattt ctttggcctg cttcatgatg agagtcgtgt
    12361 ctccggcata ggccacccag tagaagacct ccgggttggt tcgtttgatc ttggtgagca
    12421 tgggggtgaa atccgaggag ccgctcttga accgctcgtc gaagaccaca ttgaatccct
    12481 tctccttcag gtatttcacc acataatccc ggctggtggt tccgtaaatc ccgtcttcgt
    12541 aggcaacggc cacggccttt ggctggggac tgatggagag caggaaatca cggaccgttg
    12601 actgacggtg ataatcccag ggatggtaat ggaaaaacca cctcttgggc ccgaattcat
    12661 gctccacttt ggtggaggaa gcccccaccc aggccacaaa gggttcgtac cgctgaatgg
    12721 cattgagctg ggcataggtg atggtgctgg agtagccgcc gatcagggcc accactttat
    12781 ccctggcgat gagcttctcc gcagcggcaa tgcccacgga gggctccccg gtatcgtctt
    12841 cgatagtcag ctccaccttt ttccccagga ctcctccggc ctggttaacc tcctccaggg
    12901 ccatcaggag gcctcgcctc tgctcttctc ccgcctgggc gaaggcggcc ttgctcaatg
    12961 gaaggaccgc cccgatttta atcgtgtcct gggcgatcgc ggttcccgcc tgtacggcga
    13021 cgataaaaag aatcaaaaat gccgctggcc cccaacacca taatcgtttc tttcctgcca
    13081 ttggaaaacc tccttgaaaa gattttatga tccatctttg catccttggc gttgtcggcc
    13141 cttgccgttt cgcggaagga aaatggaggc aaattgtttc cacaaatctc caaggaaacc
    13201 aataa
//
LOCUS       DASPRR010000559        15826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1102156, whole genome shotgun sequence.
ACCESSION   DASPRR010000559 DASPRR010000000
VERSION     DASPRR010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15826)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15826
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102156"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VEL68_20575"
     CDS             complement(<1..675)
                     /locus_tag="VEL68_20575"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HXZ38435.1"
                     /translation="MGIQIERIARDLDILNSFTTTPGKGITRFTFSEPYMRARAYVNE
                     ELRKMGARVSTTVGGNLCGRLEGSIRGIPSVMTGSHIDSVLQGGRFDGAAGVVAALEV
                     ARVMAEEKTPHRHPIDVVVFAEEEGSRFGSVMIGSRAWIGKLSSEDLHRFKDADGTSY
                     FAAMEKAGLNPGDGPLLRSGMVKGMIELHIEQSLVLENKGLPIGVVEGINGIKQFLVT
                     ITGVPNH"
     gene            complement(698..1228)
                     /locus_tag="VEL68_20580"
     CDS             complement(698..1228)
                     /locus_tag="VEL68_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38436.1"
                     /translation="MEVEFTLEEADLVALARHHMEHSPAIRRRYRIRWIGVSLAMVLM
                     GVVLYAFLSLRAPALYLGAFAAFFLVFYPYYYRWLVGRTMGKIVNARLNPKAFAPRTL
                     RATSEGLELVSAGSKMAKGWDRVSGVEVKMDRAFVAVDGEYTIVLPRRRLGDVPFQNL
                     IETIRSFAKLSSGKGA"
     gene            complement(1275..2612)
                     /locus_tag="VEL68_20585"
     CDS             complement(1275..2612)
                     /locus_tag="VEL68_20585"
                     /inference="COORDINATES: protein motif:HMM:NF022743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HXZ38437.1"
                     /translation="MAETAKSPPQASPRIVSPQMVKVYVVALVLVVLSEWIGFVRWTP
                     IEKITILLVPFVHTIWLAVIFAPQAAGRWVKVYTISDSSWAAGIILTATYPLMVRYGT
                     LVGPNVPKLLQAGVALVLQEAGNNWGSIIMAMPIAILLGLRREVIGACYSVAREPNLS
                     LIADIYGLDTPEGRGVVGTYITGTVLGTAFFSILGSVFALTAPALFHPLALAMAMGIG
                     SASMMTAGAATLSEALPQWKDQILAFAVASNLITGVTGLYASWLIGLPFAEWIYRLMG
                     KKKEPGAKVFSLTQKQERPKINLGWTFFHVMLICILTLIGNWVATKVDPVTALPGILF
                     IFIICVVGVLLARFVPIYIPTIAYIGTISLVLTMPGMPYSKEILAAVGKVNFLALATP
                     IIAFTSLSIAKDLDTFRQQGWRIVVAALITLFSVFFSAVIIAEVCLRIQGFPR"
     gene            complement(2773..4080)
                     /locus_tag="VEL68_20590"
     CDS             complement(2773..4080)
                     /locus_tag="VEL68_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXZ38438.1"
                     /translation="MNTEELKKKVCEQIDQRREEMIRVAQRMLKQPELGYREVKTAAL
                     VKEELQKLNLPTREGLAVTGVEGSLSGREPGPAVVVMGELDSVMEKKSPLADPVTGAA
                     HLCGHHIQVASMLGAAMGLVGAGASPYLSGRVKFLGCPAEEYLEIEYRLGLMAEGKIS
                     FLGGKQELIKLGYFDDVDAALMVHSFADTSEPAFLMNAAGNGFMAKFIRYEGRPSHAG
                     AAPHRGINALNAACIGIMAVHAQRETFQDDDAIRVHPIITKGGDVVNVVPSDVRLESY
                     VRGKNLDAILDASAKVDRAFKAGGDAVGAATKITNIPGYLPIFQDRNLTEIASRNGRA
                     LLGDRGALEAGFMGGSFDVGDLSHLIPVVHPFVTGTQGHLHTAEFRVTDYEAAVVLPA
                     KLMALCVIDLLANGGAEARRIKREFQPRLTKGQYLELMQRLAQ"
     gene            complement(4095..5282)
                     /locus_tag="VEL68_20595"
     CDS             complement(4095..5282)
                     /locus_tag="VEL68_20595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXZ38439.1"
                     /translation="MAGDLFKEVHKAIDREWMVGILRELILIRSENPFDDPPKEGYRE
                     KEMADYLAGHLSALGLKVETRQVRAGRPNVFGFLNGKKGQVTLMLAGHMDTARTTGYP
                     GAYDVKVAGGKMHGRGACDMKAALAAYLAVVRALKETGTRLNGNLIVCGIIDEEYQML
                     GSKAIGQSGRMADQGIIGEPTNLKICPSNKGRVSTKIITRGRAAHSSVPEKGINAIAQ
                     MSKVIQAFGDYNQELLGRRPHPLCGHGRFTPGVIHGGVQVNMVPDYCELEVDRRTLPG
                     ESRESVYAEFHERIRSAAGSDPDFVYEITEPTWLIAPNDVSPEEPVVAALRTAHQGLF
                     GKDPGVEAFVAASDAPYMGFPTVLCGPGSIAQAHTTSEFVDLQEVADAARLYLYAALR
                     LLE"
     gene            complement(5285..6532)
                     /gene="glyA"
                     /locus_tag="VEL68_20600"
     CDS             complement(5285..6532)
                     /gene="glyA"
                     /locus_tag="VEL68_20600"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXZ38440.1"
                     /translation="MTYLAETDPAVAALIQKERHRQETTLNLIAAENTAPMAVLEALG
                     SSFNNKTAEGYPGHRYHTGCQVTDEMEQLAVARAKALFGAGHANLQPHSGVNANLAVY
                     GAVLAPGDRILSMKLSHGGHLSHGDAASLTGRVFQPSHYGVNPETERLDYDVIRDMAL
                     EIRPKMLVAGASSYPRLIDYPILRSIADEVSAYLLVDMAHIAGLVAAKVIPGPVPHAH
                     FVTFTTYKTLMGPHGGVILCTEDFAKRIDRSVFPGTQGTPTLSLVVAKAVCFHLAATP
                     EFRTVQERTLRTAAFLAGELDARGYRSVSRGTDNHMVLADLRGKGLKGNKAEAALEAA
                     GILCNRNVIPFDPESILVTSGLRFGTPGISSRGMGVEEVKMIAGWIDQILSKPDDPPL
                     QQRVREKVKDLCRRFPLRDRLEG"
     gene            6652..6819
                     /locus_tag="VEL68_20605"
     CDS             6652..6819
                     /locus_tag="VEL68_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38441.1"
                     /translation="MAQDVGFIGKPLQREVWSFHGQASPLTAEDASTLQFLSLPEGIY
                     GIEREVIKKKA"
     gene            complement(7068..7604)
                     /locus_tag="VEL68_20610"
     CDS             complement(7068..7604)
                     /locus_tag="VEL68_20610"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HXZ38442.1"
                     /translation="MKAAIVYWSKSGNTEKVARAIWEGLEASGVQVTFREADGAADLD
                     WFDFDLVCVGFPSYQFQPPEPVVAYLKNKFGEYRQQGRVKLGCPRIPGKHALIFCTYS
                     GPHTGINEAIPAGKYATQFFDHLGFEVEAEWYVLGEFHGKADNSTKGRLGDIRGKPTP
                     EELRKVKEDTIRLINWLK"
     gene            complement(7747..9036)
                     /gene="tyrS"
                     /locus_tag="VEL68_20615"
     CDS             complement(7747..9036)
                     /gene="tyrS"
                     /locus_tag="VEL68_20615"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXZ38443.1"
                     /translation="MSRNVVDVLEERGFIEQITDTGLREAASTQTLKCYVGFDPTARS
                     LHLGHIIPVMGLAHFQRNGHIPIALVGGGTGMIGDPSGKTVERQLLSKEDVEANGQRL
                     KAQLSRFFSFEGPQAALLLNNAEWLLPLNLVDFLRDIGKHFSINAMIAKESVRWRLEE
                     RGQGISFTEFSYMLLQAYDFYHLYEHHGCAVQAGGKDQWGNITAGIDLIRRMSGGSAH
                     GLTFPLLTTASGVKIGKTEGASGSEMVWLDPEMTSPYRMYQYWVNTDDRDVVKFLKLF
                     TFLDLPEITELEESLKRDPGKREPHRRLAFEFTRLAHGEEAGRAAQEASEVLFGSEVQ
                     TLSQEVFAALAGEVPATKVSKARVRNGVPLVDLLLETNLVKSKRAARDLIGQGGAYVN
                     NKTWKDLEGKVGFEQALSGRGILLRVGKKNYHLLMIE"
     gene            complement(9223..10800)
                     /locus_tag="VEL68_20620"
     CDS             complement(9223..10800)
                     /locus_tag="VEL68_20620"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXZ38444.1"
                     /translation="MIIPLTPVRFFEYVEKIFQDKEGVVCEGERFTYGEFCRRTRKVS
                     NALAKIGVQRGDRVAYLGYNCHRLLELFYAPPIMGAILEPLNIRLAAKDFDYIINEST
                     PGVLFLDKDFIPIIDSIRAKIPSVKHFLALEKTPGLPGWIQGNYEELLQGASAERTYP
                     LGKYPFEEDDPAEIFYTSGTTGNPKGVMMTHRNLYLHALCAMSMMAVRETDIQIHLIP
                     LFHVNGWGTPHYLLAKGGKHIMVKRFDPVKTLEVFEKEKVTRAFVIPTMVNALLAVPN
                     FKSFNLSRMEEILIGGAPPPRGMSERVEKAFGCICHGGFGMTETCPLIVLPELSEELD
                     AEEYRHRRHDTWGRPLPGVEFRLVDGNGKDLPWNGKSVGELLVRGDMVMKGYYRQPEE
                     TARTLEGGWYHTGDLCTLEPDGQLIVKDRKKDIIISGGENISSLEIEQVLYGHPAVLE
                     CAVIGKNDEKWGEVPLAFIVLREGYQVTPEEFIKYARDNMAHFKAPREVRILKEFPKG
                     GTGKILKSVLREKYRNT"
     gene            11149..11610
                     /locus_tag="VEL68_20625"
     CDS             11149..11610
                     /locus_tag="VEL68_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HXZ38445.1"
                     /translation="MEKIISGGQTGVDRAALDAAIDLGIPCGGWCPQGRRAEDGRIPD
                     RYPLQEASSPDYQVRTRLNVEDSDGTLVITAGSPKGGTALTLKLARKFRKPFLLVDLT
                     RKTDPSAVWQWINETHVRILNVAGPREGESAGIFEKALAFLLEVLTSPSAK"
     gene            complement(11691..13037)
                     /locus_tag="VEL68_20630"
     CDS             complement(11691..13037)
                     /locus_tag="VEL68_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXZ38446.1"
                     /translation="MADDLLAKELKEYYRKTPKARKYYQKACRYLPGGTTRTLSFFQP
                     YPLFIEKGKGCKIYDVDGNERIDFFNNATSLILGHANPAVVKAVRERSKLGTAFHAPT
                     MHEVELARLLCERIPSVERVRFMNSGTEAAMMAIRAAKAFTGKNKIGKMEGGYHGSAD
                     HAYISVHPDLAQAGPTTSPLSLSDSMGISACTMGEVVVMPFNDSEAIDQIVKRDKDNL
                     ACIIVEPMMGAAGIIPARNGFLQDLRRITRENDVLLIFDEVQTLRHSWGGAQEMHGVL
                     PDLTALAKIIGGGFPSGAVGGKAEIMGVFDSSGGRAKVPHGGTFNGNPVTMAAGLATM
                     KQLTRKSFGHLSRLGEELRHGLTGLFDRYGYRARVTGETSFFKVHFTDQEVFDYRSAQ
                     RGVNKEEERKIFFHLLNRGVFVESQIRGCLSVPMGSAEVHALLEAMEDYLRKRKSL"
     gene            13393..14730
                     /locus_tag="VEL68_20635"
     CDS             13393..14730
                     /locus_tag="VEL68_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXZ38447.1"
                     /translation="MIFSRVNHLLFSAGALLHSGAQVQAKWEMEAGRTILSCRKRVLL
                     GASLPALIFMGLNLAIAEGIPERIPILGGSRAYIPVFVTPAMFYGSILIGLLAGLITG
                     VIGAGGGYILTPALMSFGVKGIMAVGTDQFHLFAKGIMATTIHHKLGNVNVRLAVWFV
                     VGSFFGVTLGGKVNRAVYLLNPLLSDALISSVYVVVLGLLGIYSLADWLRLRRRESNA
                     RSATDATTQFARWLQGLPLKPRVRFDEDMVPGGRSISVYPVMLCGFVVGFVAAIMGVG
                     GGFLTFPMFVYGLGVSTFTTVGTDVLQIIFTTFYSSIFQYAIYGFVFYTVAMGMLLGS
                     LIGVQIGAMVTKVVTGAAIRAFYAITILAGFSNRVCALLRKLSDMGYLALPRETAALI
                     ETVGTVIFFGFLSIFGVWVLAVFFKNLDAFRNPGVVGAGFKPAPTDTTPEPQR"
     gene            14800..15513
                     /locus_tag="VEL68_20640"
     CDS             14800..15513
                     /locus_tag="VEL68_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38448.1"
                     /translation="MMIQNRKKFWVGASGLIFFSMILGFWLSPWGPHRSGLERVDQLF
                     NQLAKNSTYFIPEAVNLAPKFEGKSVDFGITSRWPGDDTRMAQIIQTNLFSAVLLGDG
                     RVRIKGDLGKMGLAASVDAGLLFKGQESDLRVKYGVNGKEVVYYWWVAFDGLTRRFVQ
                     ENRSSEADYTRFLTTRVLEPAYNFAGIETRPIGENIWTVALFLGFYLLYTLLYGFSIL
                     FLFEGLGIQATKSKEKKEA"
     gene            complement(15645..>15826)
                     /locus_tag="VEL68_20645"
     CDS             complement(15645..>15826)
                     /locus_tag="VEL68_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferridoxin"
                     /protein_id="HXZ38449.1"
                     /translation="CGACAQNCPAGAIVVKSGVGCANAVINSMLGRKSDSCCCVVEEK
                     GTASEACADSKVCCG"
BASE COUNT         3113 a         4598 c         4546 g         3569 t
ORIGIN      
        1 gtggttggga acaccggtta tagtaaccag aaactgcttg atcccgttga tcccctccac
       61 cacaccaatc gggagcccct tattctcaag cacaaggctc tgctcgatat ggagttcgat
      121 cattcctttt accatgccgg acctcagcaa agggccatcc ccggggttga ggccggcctt
      181 ttccatggct gcaaagtagc tggtcccgtc cgcatcctta aaccgatgca ggtcctccga
      241 agagagtttc ccgatccagg ccctgctgcc gatcatcacc gagccaaacc gcgatccttc
      301 ttcctccgca aagacgacca catcgatggg gtgccggtgg ggggtctttt cctccgccat
      361 caccctggcc acctccaagg cggctaccac ccctgctgca ccgtcaaacc gccccccttg
      421 aaggacgctg tcaatgtgag agcccgtcat caccgaggga attcctcgga tgcttccttc
      481 gaggcggccg caaaggtttc ctcccacggt ggtcgagacc cgcgccccca tttttctcaa
      541 ttcctcgttg acatacgccc gggcccgcat gtatggctct gagaaagtaa aacgggtgat
      601 ccctttgccc ggggttgtag tgaatgaatt gaggatgtcg aggtccctgg cgatgcgttc
      661 gatctgaatt cccatggctt gctccaagac ggatgtctca ggctcctttt cccgaggaca
      721 gcttggcaaa gctccgaata gtctcgatca ggttttggaa cggtacatct cccaggcgcc
      781 gcctgggcag cacgatggtg tattcaccgt caacggcgac aaaggcgcga tccattttca
      841 cctcgacccc gctgacgcgg tcccatcctt tggccatctt cgaaccggcg ctgaccagct
      901 ccagcccctc cgatgtcgcc ctgagtgttc gaggggcaaa ggccttcggg ttcaggcgcg
      961 cgttcacgat cttgcccatg gtccgtccca ccagccagcg gtagtaatac gggtagaata
     1021 cgagaaaaaa ggcggcaaac gcgcccagat aaagggccgg cgctctgagc gagaggaagg
     1081 catagagtac tacccccatg aggaccatgg ccagcgaaac cccgatccag cggatgcggt
     1141 agcgccggcg gatggccggg gaatgctcca tatggtgtct ggccagggcc accaggtcgg
     1201 cttcttccag cgtgaactcg acttccattc tcaaactcct gggaaacgct ctctccaccg
     1261 gtggcgctag gagatcatcg cgggaagccc tggatgcgca aacacacctc ggcaatgatg
     1321 accgcgctga agaacaccga aaacagggtg attagggccg ccaccacaat acgccagccc
     1381 tgctggcgga acgtgtccag gtccttggcg attgaaagac tggtgaaggc gatgatgggg
     1441 gtggccagcg ccaggaagtt gaccttgccc accgccgcca ggatctcctt ggagtaaggc
     1501 atgcccggca tagtcagcac cagcgaaata gtgccgatgt aggcgatggt cgggatgtag
     1561 atcggcacga aacgcgccaa cagcacaccg acgacgcaga taataaaaat gaagaggatg
     1621 ccgggcagcg ccgtcacggg gtcgaccttg gtggccaccc agttgccgat cagggtcagg
     1681 atgcagatca gcattacatg gaagaaggtc catcccaggt tgatcttggg gcgctcctgc
     1741 ttctgggtca gcgaaaagac cttggcccca ggctccttct tcttgcccat gaggcggtag
     1801 atccattccg caaagggcag gccgatcagc caggaggcgt aaagcccggt gaccccggtg
     1861 atcaggttac tggcgacggc gaaggccagg atctggtcct tccactgggg cagggcttca
     1921 ctgagggtcg ccgcgccggc ggtcatcatg ctggcgctgc cgattcccat cgccatggcc
     1981 aaggccagcg ggtggaagag ggccggcgct gtgagggcga agacgctgcc gaggatgctg
     2041 aagaaggcgg tgcccaggac ggtgccggtg atataggtcc cgaccactcc gcggccctcg
     2101 ggcgtatcga ggccgtagat gtcggcgatg aggctcaagt tcggttcacg ggcaaccgaa
     2161 tagcaggccc cgatgacttc ccgtctcaag cccagcagga tggcgatggg catggccatg
     2221 atgatggagc cccagttgtt accggcttcc tgcaggacga gggcaacgcc ggcctgcaga
     2281 agcttgggga cattgggccc gaccagcgtg ccgtaccgga ccattaaggg ataggtggcc
     2341 gtcaggatga tcccggctgc ccaggaggaa tcgctgatgg tgtatacttt cacccagcgc
     2401 cctgccgcct ggggtgcaaa gataaccgcc agccagatgg tgtgcacgaa ggggaccaag
     2461 aggatggtga tcttctcgat aggcgtccaa cgcacaaagc cgatccattc gctcaggaca
     2521 accaggacca gagcaacgac atacactttt accatctggg gggagacaat cctgggggat
     2581 gcctgaggtg gagatttcgc tgtttcggcc ataaagaacc tcctttgagt ttagggtatg
     2641 ttggggagtt taatgattgg tattaactga aaacccattg tatccaacag cgccgtgtcc
     2701 ttattagcat ttatttgact caaagaaaat ctcccatctc cttgcgcaca ttggctaacg
     2761 cctatcggcc gtctactggg ccaggcgttg catgagttca agatactgcc ccttggtcag
     2821 acgcggctgg aattctcttt tgatgcgccg ggcctcagcg ccgccgttgg ccaagaggtc
     2881 gatgacgcac agggccatca gtttggccgg cagcaccacc gcggcctcgt agtcggtcac
     2941 acggaactct gcggtgtgca ggtgcccctg ggtgccggtc acaaacgggt ggacgacagg
     3001 gatgaggtgg gacagatcgc ccacatcaaa ggaccccccc atgaagcctg cctccagggc
     3061 gccccggtcc cccaacaggg cccggccgtt tcggctggcg atctctgtga ggttccggtc
     3121 ctggaagatc ggcaggtacc cggggatgtt ggtgatcttc gtagccgcgc caaccgcgtc
     3181 ccccccggcc ttgaacgccc ggtccacctt agccgaggcg tcaaggatgg catcgaggtt
     3241 cttgccgcgc acatagctct cgaggcggac atccgagggc acgacgttga ccacgtcccc
     3301 gcccttggtg atgatcgggt gcacacggat ggcgtcatcg tcctggaagg tctcgcgctg
     3361 ggcgtggacg gccatgatgc cgatgcaggc cgcattaagt gcgttaatgc cccggtgagg
     3421 cgccgccccg gcatgcgagg gtctgccctc gtagcggata aatttggcca tgaatccgtt
     3481 gccggcggcg ttcatcagaa aagcgggttc ggacgtgtcg gcaaaggagt ggaccatcag
     3541 ggctgcatcc acatcgtcga aatagccgag cttgatcagc tcctgcttac cgcccaggaa
     3601 gctgatcttg ccctcggcca tgagccccag ccggtattcg atctccaggt attcttccgc
     3661 cgggcagccc aggaatttca cccttccgga caggtaaggg gaggctcccg cgccgaccag
     3721 ccccatggcg gcgcccagca tgctcgccac ctggatgtga tggccgcaca ggtgcgcggc
     3781 gcccgtgacc gggtcggcca ggggggactt tttctccatg actgaatcga gctcgcccat
     3841 aaccaccact gccggccccg gttccctccc ggagagcgat ccttcgaccc cggtgacggc
     3901 gagcccctcc cgggtgggca ggttcagctt ctgcaattcc tccttgacca gcgcggcggt
     3961 cttgacttcg cggtacccga gctcgggctg cttcagcatc cgctgggcca cccggatcat
     4021 ttcctcccgg cgttggtcga tttgctcgca gaccttcttt ttcagctctt cggtgttcat
     4081 gacggttcct ctcctcattc gagcagccgc agggccgcgt acaggtaaag ccgggcggca
     4141 tccgcgactt cctggaggtc gacaaactcg gaggtggtgt gggcctgggc gatggacccc
     4201 ggcccgcaga gcacggtggg gaagcccatg tagggcgcat ccgaagcggc cacgaaggcc
     4261 tccacccccg ggtctttgcc gaagagccct tggtgagccg tccgcagggc ggccaccacc
     4321 ggctcttcgg gggagacgtc gttgggcgcg atcaaccagg tgggctcggt gatctcgtag
     4381 acgaaatcgg ggtcgctgcc ggccgcactg cggatgcgct catggaattc cgcatagacg
     4441 ctctcgcggg actcccccgg cagggtgcgg cggtccacct cgagctcgca gtagtcgggt
     4501 accatgttta cctggacgcc tccgtggatc acccccggtg taaagcggcc gtggccgcag
     4561 aggggatgtg gcctgcggcc gagcagctcc tggttgtagt cgccaaaggc ctggatcacc
     4621 ttcgacattt gggcaatggc gttgatcccc ttttcgggca cacttgaatg cgcggcccgg
     4681 ccgcgggtga tgatcttggt gctaacccga cccttgttgg aggggcagat ttttaggttg
     4741 gtgggctcgc cgatgatgcc ttgatcggcc atgcgtcccg actggccgat agccttggag
     4801 ccgagcatct ggtattcctc gtcgatgatg ccacagacga tcaggttgcc gttcaggcgt
     4861 gtgccggtct ccttgagcgc tctgacgacg gcaagatagg cggcgagagc ggccttcatg
     4921 tcgcaggctc cccggccgtg catcttgcct cccgccacct tcacgtcgta ggcccccggg
     4981 tatcccgtcg tgcgagctgt atccatgtgg ccggcgagca taagggtaac ctgacctttt
     5041 ttcccgttga ggaacccgaa cacattcggc cggccggcgc gcacctgccg ggtctcgacc
     5101 ttcagcccca gggcggacag atgcccggcc agatagtcgg ccatctcctt ttcgcgatag
     5161 ccttccttcg gtgggtcgtc gaaggggttc tcgctgcgga tcaggatcaa ctcccgcaga
     5221 atgccgacca tccattcccg gtcgatggcc ttatgcacct ccttgaaaag atctcctgcc
     5281 atggtcagcc ctccaatcgg tcgcgcaggg ggaaccggcg gcagaggtct ttaaccttct
     5341 ctctcacccg ctgttgtaga ggtggatcat ccggcttgga gaggatctgg tcgatccagc
     5401 cggctatcat tttcacctcc tccactccca taccgcggct ggagatgccg ggtgtgccga
     5461 agcgcaggcc gctcgtcacc aggatggatt ccgggtcgaa ggggatgacg ttgcggttgc
     5521 aaaggatgcc ggcagcctcc agcgccgcct cggccttgtt ccctttgagc cctttgcccc
     5581 gcaggtccgc gagcaccatg tggttgtcgg tgcccctgct caccgatcgg tatcccctgg
     5641 cgtccagttc gccggcgagg aaggcggctg tgcgcaaggt acgttcctgg accgtgcgaa
     5701 actccggcgt cgcggctagg tgaaagcaca ccgccttggc gactacaagc gatagcgttg
     5761 gcgtgccttg ggtgccaggg aagacggagc ggtcgatcct tttggcgaaa tcctcggtgc
     5821 acaggatcac cccgccatgg ggccccatca gggtcttgta ggtggtgaag gtcacgaagt
     5881 gggcgtgggg cactgggccg gggatcacct tagccgccac cagcccggcg atgtgggcca
     5941 tgtccaccag gaggtaagcg gagacctcat ccgcgatgga acgcaaaatc gggtaatcga
     6001 tcaggcgcgg gtaggagctt gcgccggcca cgagcatttt gggacggatt tcaagcgcca
     6061 tgtcgcggat gacatcatag tccagccgct cggtctcagg gtttacgcca taatgcgatg
     6121 gctggaaaac ccggccggtg agggaagccg cgtctccatg ggagaggtgg ccgccgtgac
     6181 tcaacttcat gctgaggatc ctgtcacccg gggccagcac agccccatag accgccaagt
     6241 tggcgttgac cccggaatgc ggctggaggt tggcgtggcc cgcgccgaaa agcgccttgg
     6301 cccgtgctac ggccagctgc tccatttcat ccgtaacctg gcagcccgtg tgatagcggt
     6361 gccccgggta gccctcggcg gtcttgttgt tgaagctcga acccagagct tccaggaccg
     6421 ccatgggcgc tgtgttctcg gcggcgatga ggttgagggt ggtttcctgg cggtggcgct
     6481 ctttctggat cagggcggca actgcggggt cagtttcagc gaggtatgtc atcctgccct
     6541 cagcgctgga ggctgtcgac taattgatga aaacaagccg ggacgaagtg caggcctgaa
     6601 tcttttttgt acgagagtat actgatttgg aaattttgcg tcaaggaaat tatggcccaa
     6661 gatgttgggt ttattggaaa gccgcttcag cgggaagtct ggagtttcca cgggcaggcg
     6721 tccccgctga ccgcggagga tgccagtacg ctgcagttcc tttccctgcc tgagggaatc
     6781 tatggaatcg aacgggaggt aatcaagaaa aaggcttaag actcgttgtt ggttactggt
     6841 tggtcgttgc cggctgctag ttaccaatac aaacgcatta aatatcttac catgtcattg
     6901 cgagcccgaa gggcgtggca atctcatttt tattcgggat tgcttcgtcg ctccgctcct
     6961 tgcaatgacc agttatttat tgcgtttgta ttaggcagcg gttctttgtt actcctttct
     7021 tgttttttat acatcggtaa cgagtaaccg caataagtaa caagcaacta cttcagccag
     7081 ttaatcaacc ggatggtatc ctccttcacc ttccgcagtt cttccggggt gggttttccg
     7141 cggatgtctc ccaatcggcc cttggtgctg ttgtctgcct tgccgtggaa ttctccgagc
     7201 acataccatt ctgcttccac ctcgaatccc aggtgatcga agaactgggt tgcatatttt
     7261 cccgcaggga tggcttcgtt gatcccggta tgcgggcctg aataggtgca gaagatcaga
     7321 gcatgtttcc cggggatgcg cggacaccca agtttcaccc tcccctgctg tcggtattcc
     7381 ccgaatttgt tcttcaagta ggcgacaacc ggttcggggg gttgaaactg gtaggagggg
     7441 aaccccacgc acaccaggtc aaaatcgaac cagtccaggt ccgccgctcc atcagcctcc
     7501 cggaaagtca cttgaacccc tgaggcctcc aacccttccc agatggccct ggccaccttt
     7561 tccgtgtttc cgctcttgga ccaataaaca atagccgcct tcataggtca aaacctcgtc
     7621 gctggtttct tgatgccggt tgatggttgc tcgttattag ttcctgttta aatgtacctg
     7681 tttgccggcc gctttttcgc cttcttcagt tttatcagcc ggcaacgagc aatgagaaac
     7741 tggaagctat tcaatcatca agagatgata attcttctta cccacccgca ggagaatccc
     7801 ccggccggat aacgcctgtt cgaacccgac cttgccttcc aggtccttcc aggtcttgtt
     7861 attcacatag gcgccgccct gaccgatgag gtctcgggcc gcccgtttgg atttgaccag
     7921 gttggtttcc aggagaaggt ctaccagcgg gacaccattc cggaccctcg ctttggagac
     7981 cttcgtcgcc ggcacttccc cggccagagc ggcaaaaacc tcctgggaga gggtctgaac
     8041 ctcgctcccg aagagaactt ccgaggcctc ctgcgcggcc cgccccgctt cttccccgtg
     8101 agccagcctg gtgaattcga aggccagacg gcggtggggt tcccgcttcc cggggtcccg
     8161 tttcagcgat tcttcgagtt ccgtgatttc gggcaaatcc aggaaggtga agagcttgag
     8221 gaacttgacg acgtcgcggt cgtccgtgtt aacccagtac tggtacatcc ggtaaggaga
     8281 ggtcatctcc gggtctagcc agaccatttc gcttccggat gcaccttcgg tcttgccgat
     8341 cttcaccccc gaggcagtgg ttaaaagggg aaaggtgagc ccgtgggcgc tccccccgct
     8401 catccggcga atgaggtcga tgcccgcggt gatgtttccc cactggtcct tccctccagc
     8461 ctgcaccgcg cagccgtgat gctcatacag gtggtagaag tcataggcct ggagaagcat
     8521 atagctgaac tcggtgaaag agatgccctg ccccctttct tccagacgcc agcggaccga
     8581 ctccttggcg atcatggcat tgatcgaaaa gtgtttcccg atatcccgca agaaatcaac
     8641 caggttaagg ggcaagagcc actcggcgtt gttcagcagg agggccgctt gaggcccctc
     8701 aaaactgaaa aaacgggaaa gctgcgcttt caggcgctgg ccattggctt ccacgtcctc
     8761 tttagaaagg agttgtcttt ccaccgtctt cccgctggga tccccgatca tccccgttcc
     8821 ccctcccacc agggcgatgg gaatgtgccc gttgcgctgg aaatgagcca gccccatgac
     8881 cgggatgatg tgacccaggt gaagggacct tgcggtagga tcaaagccta catagcactt
     8941 taaggtctga gttgaagccg cttcccgcaa accggtatcg gtaatctgtt cgatgaaacc
     9001 tctctcttcc aaaacatcga cgacgttcct ggacaaaacc gcctccttaa gtgcctatag
     9061 ttcggagtgc ctagagtgcc tggaatcaag aatccaggac ccagaattca ggagaaatga
     9121 tgaacgaaat tattcttctc ttcttctggc ttctggattc tggtttctga attctgtttc
     9181 tcccattccg cattcctcaa tggacaattc gcaatgcgca attcaagtgt tcctgtactt
     9241 ttccctcagc accgatttca gaatcttccc cgttcccccc ttgggaaact cctttaggat
     9301 cctcacttcc cgaggagcct tgaagtgggc catgttgtcc cgggcatact tgatgaactc
     9361 ctccggagtc acctggtagc cttcccgcaa aacaataaag gccaggggta cctcgcccca
     9421 cttttcgtcg ttcttgccga tgaccgcgca ctccagcacc gcaggatggc cgtagagaac
     9481 ctgctcgatt tccagggagg agatgttttc tcccccgctg atgatgatat ctttctttcg
     9541 gtctttgacg atcagctgcc cgtccggctc cagggtgcaa aggtcgccgg tgtggtacca
     9601 cccgccctcc agggtccggg ccgtctcctc tggttggcgg taataccctt tcatcaccat
     9661 gtctccccga accagcagct ctcccacgct cttaccattc caggggaggt ctttcccatt
     9721 cccatccacc aggcggaatt ccacccccgg aaggggcctt ccccaggtat cgtggcggcg
     9781 gtgccgatat tcctccgcgt ccagttcttc ggacagttcc ggcagaacga tcaaggggca
     9841 ggtctcggtc atcccgaacc cgccatggca gatacagccg aaagccttct ccacccgttc
     9901 ggacattccc cgcggcggcg gcgcgcctcc gatgaggatt tcttccatcc gggaaaggtt
     9961 aaaagattta aagttcggta ccgccagcag cgcattgacc atggtgggaa tgacgaaggc
    10021 ccgggttact ttctcctttt caaaaacctc cagggttttg acgggatcga aacgcttcac
    10081 catgatgtgc ttgcctccct tcgccaggag gtaatggggc gttccccagc cgttcacgtg
    10141 gaaaaggggg atcaggtgaa tttggatatc cgtctccctg acggccatca tggacatggc
    10201 gcacagggcg tggagataga gattgcggtg ggtcatcatc acccctttgg gatttccggt
    10261 cgtcccgctg gtataaaaga tttccgccgg gtcatcctct tcgaaaggat acttccccaa
    10321 aggatacgtt cgttcggcgg aagcaccctg caagagctct tcgtaattcc cctgaatcca
    10381 acccggcagc ccgggggtct tttccaaagc gaggaaatgt ttcactgaag gaatctttgc
    10441 ccgaatggag tcaatgatcg ggataaagtc cttatccagg aaaagcaccc cgggagtcga
    10501 ttcattaata atgtaatcga agtccttggc cgccagccga atgttcagcg gttccaggat
    10561 ggctcccatg atcgggggcg catagaaaag ctccaggaga cggtggcagt tgtatcccag
    10621 gtaagcgacc cggtcccccc tttgaactcc tattttcgcg agggcgttgc tgactttcct
    10681 tgtccgccgg cagaactccc cgtaggtaaa tctctcccct tcgcacacca ctccttcttt
    10741 atcctggaaa atcttctcca cgtattcgaa aaagcggact ggcgttagag gtatgatcat
    10801 ctgcgccccc tttatctcgg cctggatcgg tatgaaaaga atcctagagg acaaaataat
    10861 gtcctattat cctcaaaaag attggattaa tcaagatttt cggaggcttt atgcggcgaa
    10921 gaaagaaaaa tcttttgtta aagattggga aatatgctag ggagaaagca gcaataaaaa
    10981 aggaaaacgg gaggcggata aaccgctgag cgctagtaat cacctctggg aaggtttgtt
    11041 tgagctttga gggttgtaaa acccttctga ttcgtaagaa cgatatgtac gggttctctg
    11101 cataatttta gcggtcggcg gattaacttc ctgataatga aaaatgtatt ggaaaaaatt
    11161 atctccggcg ggcagaccgg ggtggaccgg gcggcactgg atgcagccat tgacctgggg
    11221 attccctgcg ggggttggtg cccccagggc cggagagcag aggatggaag gattccggac
    11281 cgctacccct tgcaggaggc ctcctcaccg gactaccagg ttcggacgcg gctgaacgtg
    11341 gaggattcgg acggcaccct ggttatcacg gcagggtctc cgaaaggggg aaccgccttg
    11401 accctgaaac ttgccaggaa gttccgcaaa ccctttcttc tggtcgacct gacccggaag
    11461 acagacccat ccgcagtttg gcagtggatc aacgagaccc atgtgcggat tctgaatgtg
    11521 gccgggccac gagaagggga atctgcgggg atcttcgaga aggctttagc ctttttactg
    11581 gaggttttga cctccccttc cgcgaagtaa ggatttcggg ggaaatcggt ggcctggcac
    11641 tcctcttttt gaaacgaagt cgaaaggcca ggttgattca actctgagtt ttaaagactt
    11701 ttccttttcc tcaggtaatc ttccatcgcc tccaagagag catggacctc ggcgcttccc
    11761 atgggaaccg agaggcatcc tctgatctga gactcgacaa agaccccccg gttcaaaagg
    11821 tggaagaaga tctttctctc ttcttctttg ttgacccctc tctgcgcgct ccggtaatca
    11881 aatacttcct gatccgtaaa gtgaactttg aaaaatgagg tttctccagt cacccgggct
    11941 ctgtatccgt aacggtcgaa gaggccggtt aaaccgtggc ggagttcttc ccccagcctg
    12001 gagagatggc caaaggattt ccgggtgagc tgcttcatgg tggccaatcc agcggccatg
    12061 gtgacggggt ttccattaaa ggtccccccg tggggaacct tggcccgtcc tccgctggaa
    12121 tcaaagaccc ccatgatttc ggccttgccc cccacagccc cgctgggaaa ccccccaccg
    12181 atgatcttgg caagggctgt cagatcggga aggaccccat gcatctcctg ggccccgccc
    12241 caggaatggc gcagggtctg gacctcgtca aaaatgagca ggacatcgtt ttcccgggtg
    12301 atccgccgca aatcctgaag gaaaccgttc ctggccggga tgatgcccgc cgctcccatc
    12361 atgggttcga cgatgatgca ggccaggtta tctttgtctc tttttacgat ctggtcgatg
    12421 gcctcggaat cgttgaaggg catgactacc acttcgccca tggtgcaggc cgaaattccc
    12481 atggaatcgg aaagggatag gggcgaggtg gtgggcccgg cctgagccaa atccgggtgc
    12541 acgcttatgt aagcgtggtc cgccgatccg tggtaccccc cttccatctt gccgatttta
    12601 ttctttccgg taaaggcctt ggccgcccgg atggccatca tggccgcttc cgttccgctg
    12661 ttcatgaacc tcacccgctc caccgaggga atccgctcgc agagcagccg ggccagttcg
    12721 acctcatgca tggttggagc atgaaaggcc gttcccaatt tcgacctctc ccgcaccgcc
    12781 ttcaccaccg ccgggttggc gtgtcccaga atgagggagg tggcgttgtt gaaaaagtcg
    12841 atccgctcgt tgccgtccac gtcgtatatc ttgcagccct tacccttctc gataaacaag
    12901 ggatagggct ggaagaaaga aagcgtccgc gtcgtccccc cggggagata ccggcaggcc
    12961 ttctggtagt acttcctggc cttgggagtc tttcggtaat actctttcag ctccttcgcc
    13021 aaaagatcat ctgccatctt ggaacctccg tccttccgga gccactcacg aatggcccgc
    13081 cggatggatc aatatactct cttccctgct ggtcgtagag gtatgttcct tctccccttg
    13141 gtcctgagtt ttctccttgg tcttaatcgc tggggtaatt cgcggacagt ttatttgcgg
    13201 aaatttttat atggctaatg gtgagcaaga ttatttatgc tctggacctt aatgtcaaga
    13261 agtttttggg tcaaattccg ggaggcaaga gagaaatgag acccttgaaa ttcaccgggg
    13321 cgcaaagtag tatggcaaga gttaggttca cgtttgaaag attttttaaa ctcgaatttt
    13381 gaaggtcttt ttatgatttt cagcagggtc aaccatcttc ttttctcggc cggagcactc
    13441 ctccattcag gggctcaggt gcaagccaag tgggagatgg aagctggccg taccattctt
    13501 tcctgccgca aaagagttct gttgggggct tccctccccg ccctgatctt catgggcttg
    13561 aacctggcta tcgccgaagg catccccgag cggattccga ttctcggcgg gtcgagggcc
    13621 tatattccgg tcttcgtgac cccagccatg ttttacgggt ccatcctgat cggtctcttg
    13681 gccggtttga tcaccggggt gattggtgcg ggagggggat acattctgac ccctgccctc
    13741 atgagcttcg gggtgaaggg gatcatggca gtgggcacgg accagttcca cctttttgcc
    13801 aaggggatca tggccacgac catccaccac aaactgggaa atgtgaatgt ccgactcgcc
    13861 gtctggttcg tggtcgggtc cttcttcgga gtcactctgg ggggaaaggt caaccgagcg
    13921 gtctacctgc tcaatccgct tctcagcgat gccctgatca gcagtgtcta cgtggtggtc
    13981 ctgggtcttt tgggaattta ttctctggcc gactggctcc ggctgagaag gcgggagtcg
    14041 aatgcccgtt cagccaccga tgcgacgact caatttgccc ggtggctcca ggggcttcct
    14101 ttgaaaccca gggttcgctt cgatgaagac atggtccccg gcgggcgcag catctctgtt
    14161 tatccggtca tgctttgcgg attcgtggtc ggcttcgtgg ccgccatcat gggggtcggc
    14221 ggcggattcc taactttccc catgttcgtg tatggccttg gggtctccac cttcaccacc
    14281 gtgggaacgg atgttttgca gatcattttc accactttct actcttccat cttccagtac
    14341 gccatctatg gatttgtttt ttacaccgtg gccatgggga tgctcctggg ttcgttgatc
    14401 ggggtgcaga tcggcgcaat ggtgaccaag gtcgttaccg gggcggccat tcgggctttt
    14461 tatgcgatca cgattttggc gggtttttct aaccgcgtct gtgccctcct ccggaagctc
    14521 tcggatatgg gctacctcgc acttccaaga gagacggccg ctttgatcga gaccgtgggg
    14581 acggtcattt ttttcggttt cttgagcatt ttcggggtct gggtccttgc cgtgttcttc
    14641 aagaacctgg acgctttccg caatcccggg gtcgtggggg cgggttttaa acccgccccc
    14701 acggatacta ccccggagcc gcagagatag ccttgttcaa aagccccatc tagcgaggtt
    14761 cccggagaca gtgggcacag gacaacaggc attcgaagga tgatgatcca aaaccggaaa
    14821 aaattctggg tcggcgcgag cggcctgatc ttcttttcca tgatccttgg cttttggctt
    14881 tctccctggg gacctcatcg gtcggggctg gaacgggttg atcagctgtt caaccagctg
    14941 gccaagaatt ccacttattt cattcccgag gccgtaaatt tggcccccaa gttcgagggc
    15001 aaaagcgtcg atttcggaat cacttcaaga tggccgggag atgatacgcg gatggcccag
    15061 attattcaga cgaacctttt ctcggctgtc cttctgggtg acggaagagt gcggatcaaa
    15121 ggggacctgg ggaaaatggg gctggccgca tcagtggatg cgggattgct tttcaagggg
    15181 caggaatcgg acctccgggt caagtatgga gtcaacggga aagaggtggt ctattactgg
    15241 tgggtggcct tcgatggcct gacccgccgg ttcgtccagg agaaccggtc ttcggaggcg
    15301 gactataccc gattcctgac tactcgggta ctcgagccgg cctacaactt tgcgggaatt
    15361 gaaacccggc caattgggga aaatatctgg acggtcgccc tcttcctggg tttttatctc
    15421 ctttacactc tcctgtatgg attctccatt cttttcctct ttgaggggct cggaatccag
    15481 gccaccaaat cgaaggagaa gaaggaagcc taaagttcgt tactcgttgc ttgttgctgg
    15541 ttgctcgttc ctggatgctg attgtccgat gtcctttaag ggtgcccttc catctaccgt
    15601 caaatatctg cgattccata cccagaatct accatccggc ccggtcatcc gcagcacact
    15661 ttgctatccg cgcaggcttc tgaagccgtc cccttttcct cgacgacgca gcagcaggaa
    15721 tcgcttttgc gtcccagcat ggaattgatt accgcgttgg cgcatcccac cccggatttg
    15781 accacaatcg ctccggcggg acaattctgg gcgcaggccc cgcatt
//
LOCUS       DASPRR010000560         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_789828, whole genome shotgun sequence.
ACCESSION   DASPRR010000560 DASPRR010000000
VERSION     DASPRR010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789828"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VEL68_20650"
     CDS             <1..715
                     /locus_tag="VEL68_20650"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ38450.1"
                     /translation="DRTPFTSVQGKHQLVRPPGALPEEEFLRTCIRCGECMKSCVTNT
                     LQPSLWQSGLSGLWTPRMDLRFAACEQNCNACGKVCPTQAIRSVSLEEKTHAKVGTAI
                     LLKERCLVWSQDKLCLICDEICPYNAIVFRTIDSYRRPVVIASKCNGCGFCETRCPVG
                     GTSAIVVVPDGEIRLKQGSYIKEAQKLQLEFKPDPGDDKFVLESSGFKVGEKQSPAKP
                     EKPSAAPEKAPGRKPAGFL"
     gene            complement(791..1660)
                     /locus_tag="VEL68_20655"
     CDS             complement(791..1660)
                     /locus_tag="VEL68_20655"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXZ38451.1"
                     /translation="MNSASKREEENKETVKFKALYGISRVLGGALDLRESYPKVLTVL
                     AQHLGMKRGGLLLMNAESNDWEMGGSHGLSPEEMKRRKEYFGSGVIARILEKGQATAI
                     VEGGENLWVYDGKSRSVPKRGAIYFLCAPVKVQGTIAAILGADHFFEDPVPLTEDLNL
                     LGEICTLIGDAMTMRRAIAAENRALLEENWSYRKELETLGKTLPKARKRISLTEILEE
                     RISRMIAEMKVDPRSNGCLYNEVLHVVEKTLLKSSLDKTKHVQLKTARFLGINRNTLR
                     RKIKELGICAQEK"
     gene            complement(2025..>2240)
                     /locus_tag="VEL68_20660"
     CDS             complement(2025..>2240)
                     /locus_tag="VEL68_20660"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXZ38452.1"
                     /translation="CKYHGSARYDDVLIIETSVDFLKRASIQFSYRILRKSDGNQLVT
                     GSTLHAFVDRDGKIVKVPAIFRAKLNT"
BASE COUNT          511 a          635 c          560 g          534 t
ORIGIN      
        1 cgaccgaacc ccctttacct cagtccaggg aaagcaccag ctggtgcggc cccccggagc
       61 cttgcccgaa gaagaattcc tgcgcacgtg catccgctgc ggggaatgca tgaagtcctg
      121 cgtcactaac actctccagc cctccttatg gcaatcaggc ctatccggac tatggactcc
      181 tcgaatggac cttcgttttg ccgcctgcga gcagaactgt aacgcctgcg ggaaggtctg
      241 ccccacccag gccatccgct cggtctccct ggaagaaaaa actcatgcca aggtcggcac
      301 cgccatcctc ctcaaggaaa ggtgcctggt ctggtcccag gacaagctct gcctgatctg
      361 cgacgaaatc tgcccctaca acgccattgt cttccgtacc atcgacagct atcgccgtcc
      421 tgtggttatc gcttctaaat gcaatggctg cggtttttgt gaaacgaggt gccctgtggg
      481 agggacttca gccatcgtcg tagtgccaga tggagagatt cgcctgaagc aggggtctta
      541 catcaaagag gcacagaagc ttcagctcga gttcaagccc gaccctgggg acgacaagtt
      601 cgtcctggaa agttccggct tcaaggtggg agaaaagcaa tccccagcaa aacccgaaaa
      661 accttcggcc gccccggaga aggctcccgg gagaaaaccc gcggggttcc tgtaagaagc
      721 aaatcccctt gcgcgtttct agttgctcgt tgaaaaaaag aatacctaac cagtaacaag
      781 gaaccagaaa ctatttttct tgggcgcaaa ttcccaattc tttaatcttt cggcgtaggg
      841 tgttccggtt gatgcccagg aagcgggcgg tcttgagctg gacatgtttg gttttatcca
      901 gggaggattt gaggagggtc ttttccacga cgtgtaaaac ctcgttgtag aggcagccat
      961 tggaccgggg gtccaccttc atctcagcga tcatccggga aattctttcc tccaaaatct
     1021 cggttagtga aatccttttc cgcgccttgg gcagcgtctt acccagggtc tccagttctt
     1081 tccggtagct ccagttttct tccagcaagg cgcggttctc cgcggcgatg gccctccgca
     1141 tggtcatggc gtccccgatg agggtgcaaa tctcccccag aaggttcagg tcttcggtca
     1201 gcgggacagg gtcttcaaag aagtggtccg ctcctagaat cgccgcaatg gttccctgga
     1261 ctttcaccgg tgcgcataag aagtaaatcg ctcccctttt gggcacgctt cgggatttgc
     1321 cgtcatacac ccacaggttt tctccgcctt cgacaatggc cgtcgcttgt cccttctcca
     1381 ggatccgggc aattactccc gagccgaaat attctttcct tcgtttcatc tcttcgggcg
     1441 aaagaccgtg cgagcctccc atctcccaat cgttggactc ggcgttcatg agaagcaggc
     1501 cccctcgctt cattcccaaa tgttgagcga gaacggttaa gaccttcgga taactctccc
     1561 tcaggtccag ggctccgccc aggactcggg aaatcccata aagggcctta aattttacag
     1621 tttccttatt ctcttcttcc cttttggagg cggaattcat ggatattccc ttcgagagag
     1681 caaaaaaggc cccggcaccg ctcgcttgat tgatttagat tcagctcatg aatttagaga
     1741 tttcctgcaa ccccggaatc gggattggga ggagagataa aaattcaccc tcgccgattg
     1801 accccaaccc aacctataag aaataaagtc cggtgtcaag accttctttc agagaatcgg
     1861 gaaaaaattc ctccgggttt cccgcataaa aaggtcagga ctcgggtttt accctctcct
     1921 tcccgctttg gccttgatca cgtctttccg agaatttctt attgcacaag ccccgtaact
     1981 tttgcaacag aaaaaacgga aaataaaatt tttggggggg atttttaagt gttcagtttt
     2041 gctcggaaga tagccggaac tttgacgatt ttcccatccc gatccacaaa ggcgtggaga
     2101 gtggagccgg tgaccaattg gttcccgtcc gacttccgaa gaatccggta ggagaactgg
     2161 atactggccc gcttgagaaa atccacggac gtctcgatga tcagaacatc gtcgtagcgg
     2221 gccgaaccgt gatacttgca
//
LOCUS       DASPRR010000561         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_280777, whole genome shotgun sequence.
ACCESSION   DASPRR010000561 DASPRR010000000
VERSION     DASPRR010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280777"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VEL68_20665"
     CDS             complement(<1..234)
                     /locus_tag="VEL68_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ38453.1"
                     /translation="MIVPPPLLTVKDLRVRRGGVRVLEIPSLVVEEGSILSLIGPNGG
                     GKTTLLQTLACLIQAEEGEFFFKGEKVSSSHGLL"
     gene            complement(231..929)
                     /locus_tag="VEL68_20670"
     CDS             complement(231..929)
                     /locus_tag="VEL68_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ38454.1"
                     /translation="MDLIFEGILKAFYLLFTLDPEVLGITLLSLQVSGTATLISLFIG
                     ISIGTLVALTEFPGRRLVVSLINTGMGLPPVVVGLFVTIFLWRNGPLGFLEILYTPAA
                     MIVAQAVIATPIVMGITLAAIQALPKKLRLQILALGATRLQMVWILVKEARLPLLAGV
                     MAGFGGVISEVGASIMVGGNIKGYSRVLTTATVMETSRGNFDVAIALGIILLLLAYFI
                     NLVLTHIQQRERPR"
     gene            complement(930..1787)
                     /locus_tag="VEL68_20675"
     CDS             complement(930..1787)
                     /locus_tag="VEL68_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXZ38455.1"
                     /translation="MNQKKTIYAVLTALLVILGLVAWSPVQAQQKNIILSTTTSTQDS
                     GLLDVLIPIFEKKTGYFVKTIAVGSGQAMAMGQKGEADVLLVHSPEAERKFVAEGYGI
                     NRRLVMHNDFIVVGPAEDPAKIKGMKATTEVFKKIASAGSLFLSRSDKSGTHAKEMSI
                     WKAAGIKPEGQKWYQETGLGMGETLNVTSEKKGYTLADRGTYLALKKNLSLDILAEGD
                     AILLNIYHVIEVNPGKWPKLNAAGGKAFADFMVAKETQEIIKTFGVDKFGSPLFFPDA
                     GKKEEELGK"
     gene            complement(1810..2676)
                     /locus_tag="VEL68_20680"
     CDS             complement(1810..2676)
                     /locus_tag="VEL68_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXZ38456.1"
                     /translation="MTRIKRVLVFGAICLVLLAPVWCGAQSSGFVLLSSTIGPIDSGI
                     VGLLEDQFEKETGIRVRHVGAGTGAALEIAKKGNVDLVMVHAKSLEEKFVQEGFGTER
                     IGLMYNDFVIVGPANDPAGIKGMKSAQEALKRISEKEVVFVTRGDKSGTHVAEMELWQ
                     KAGIKPQGSWYYTCPRGAEGNVPTLRHTDKMEAYTFIDRATVLAMQKEIKLEVLVEKD
                     EALLNFISLIPVNPQKFPKVNYTDTMKFVKWLTSPEKGQKIIADFGKDKYGAPLFFAD
                     SKEWREKQGLKR"
     gene            complement(2689..3609)
                     /locus_tag="VEL68_20685"
     CDS             complement(2689..3609)
                     /locus_tag="VEL68_20685"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXZ38457.1"
                     /translation="MPEELMNTKEAAKHLGIHEKQVYALIKAKRIPCTRVTGKWVFPR
                     RLIDEWIENNARSGMAEARKKTKRIEGALLAAGSNDPILDILKTYIRRTYPEFYIFSA
                     NTGSTDGLKALNQGFTDIAWSHLLDPKSGQYNIPFLSTYLPNLKTVVVNLFNRDLGFL
                     VAHGNPLHVRGFKDLTRPKIRFVNRQKGSGTRVLLDHHLKDLKIPSSSIKGYENEVFT
                     HFEIGLSILAKEADVGLATGSVSKLLGLSFVPLTQESFDMLLDQSTFFERGVQAFIEI
                     LNSREFRNRVEYLGSYDFKNSGKILYSKAA"
     gene            3777..3986
                     /locus_tag="VEL68_20690"
     CDS             3777..3986
                     /locus_tag="VEL68_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXZ38458.1"
                     /translation="MKISARNVLKGKVKKLVHGAVNSEVTLEMPGGMQVVSIITKSSA
                     RNLGLKKGKTVYAVIKASNVMIAID"
     gene            complement(4059..>4405)
                     /locus_tag="VEL68_20695"
     CDS             complement(4059..>4405)
                     /locus_tag="VEL68_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38459.1"
                     /translation="GSLRIAKLIPPHYVPWVIKGTSLGAWYDIREDRYRILCAQCYCL
                     ATYKTIEIAYHETVLSEGKCCQGCRTRISFEKNHRLIGVFLDFWGRTGHFPESGSWLD
                     AIPSKQLNLWAK"
BASE COUNT          966 a         1232 c         1141 g         1066 t
ORIGIN      
        1 caggaggccg tggctggaag agaccttttc tcccttgaag aagaattctc cctcttctgc
       61 ctggatcagg caggccaggg tttgcaggag ggtggtcttg cctcccccgt ttggcccgat
      121 gagggagagg atcgacccct cctccacgac cagagaagga atttccagga cccgcacacc
      181 tcccctgcgg acacgcaaat ctttgactgt caggagcggg ggggggacga tcacctcggc
      241 ctctccctct gctggatgtg agtcagaacg aggttgataa agtaggcgag cagcaggaga
      301 atgatcccca gggcaatggc tacatcgaag tttccccggc tggtttccat gaccgtggcc
      361 gtggtcagaa cccggctgta ccccttgatg ttgccgccca ccatgatcga tgcccccacc
      421 tcggagatga ctcccccgaa cccggccatc actccggcca gaagcggcag tcgcgcctct
      481 ttgaccaata tccagaccat ctgcaggcgc gtggctccca gggctagaat ttggaggcgg
      541 agtttcttgg gaagggcctg gatcgccgcc agggtgattc ccatgacaat gggagtagca
      601 atcaccgcct gggcgacaat catggccgcc ggggtataga gaatctccaa aaaccccagc
      661 ggcccgtttc tccagagaaa aatggtgaca aagaggccca ccaccaccgg cggcaggccc
      721 attcccgtgt tgatcaggct tacgaccagc cttcttcccg gaaattccgt cagggccacc
      781 agcgtcccaa tggagattcc gatgaaaagg ctgatcaggg ttgccgttcc cgagacctga
      841 agggaaagca gggtgatccc caacacctcc gggtcgaggg tgaataggag atagaaagct
      901 ttcaggattc cttcaaaaat gaggtccatc tactttccca gttcctcttc tttctttccg
      961 gcatcgggga agaacaaggg cgacccgaac ttatccaccc cgaaggtctt gatgatctct
     1021 tgggtttcct tcgctaccat gaaatcggca aaagccttgc ccccggcagc gttgagtttg
     1081 ggccatttcc ccgggttgac ttcaatgaca tggtagatgt tgaggaggat ggcatcgccc
     1141 tcggcaagaa tatcaaggct caggtttttt ttcagggcca aataagttcc ccggtcggcc
     1201 agcgtatagc ccttcttctc cgaggtcacg ttgagggtct cccccattcc cagcccggtc
     1261 tcctggtacc atttttggcc ttccggtttg attcccgccg ccttccagat ggacatttcc
     1321 ttggcatggg ttccggattt atcactccgg gagaggaata gagaccccgc agaggcgatc
     1381 tttttgaaca cctccgtggt ggccttcatt cccttgatct tggccgggtc ttccgccggc
     1441 cccacaacaa taaagtcgtt atgcatgacc agcctccggt tgatcccgta cccttcggcc
     1501 acgaacttcc tctccgcctc cggggaatga accagaagca cgtccgcctc ccccttctgc
     1561 cccatggcca tggcctggcc ggaccccacg gcgatggtct taacaaagta gccggtcttt
     1621 ttttcaaaga tcgggatgag cacatccagc aggccggagt cctgggtgct ggtggtggtc
     1681 gacagaataa tatttttctg ctgggcctga accgggctcc aagccaccag accgagaatc
     1741 acaagcagcg ctgtcaaaac tgcgtaaatt gtctttttct ggttcatcct ttcctcctta
     1801 aagggtttct tacctcttca atccctgttt ctcccgccac tccttggagt cggcgaagaa
     1861 gaggggggct ccgtatttat ccttgccgaa atcggcaatg atcttctgcc ccttttccgg
     1921 cgaggtaagc cattttacaa atttcattgt gtcggtgtag ttcactttgg ggaatttttg
     1981 aggattcacg gggatcagag atatgaagtt taaaagagcc tcatcctttt ccaccagaac
     2041 ttctagtttg atttccttct gcatggccag gaccgtggcc ctgtcgataa aggtataagc
     2101 ctccatctta tccgtatgac gcagggtggg cacgttgccc tcggcgcctc ttgggcaggt
     2161 gtaataccag gacccttgag gtttgatccc cgccttctgc cagagttcca tttcagccac
     2221 atgggttccg gacttgtctc cccgggtgac aaaaacgact tctttctccg aaattctttt
     2281 gagggcttcc tgggcgctct tcatcccctt tattcccgcc ggatcattcg ccggacccac
     2341 aatgacaaag tcgttgtaca tgagaccgat tctttccgtc ccaaatccct cttgaacgaa
     2401 tttttcttcc agagacttgg catggaccat gaccaggtcc acgttcccct tcttggcgat
     2461 ctccagggct gcccccgtgc ccgcacccac gtggcggaca cggatgcccg tctccttttc
     2521 aaactgatcc tccagaaggc ccacgattcc ggaatcaatc ggaccgatgg tgctggacaa
     2581 aaggacaaac ccactgctct gggcgccgca ccagacgggg gcgaggagca ccagacagat
     2641 ggccccaaaa accaatactc gttttatcct tgtcattctc cactcctttt atgctgcctt
     2701 ggaatacagg atttttcccg aattcttgaa gtcgtaactc cccaggtatt ccacccggtt
     2761 ccggaattcc cgggagttaa ggatttcgat gaacgcctgg actccccgtt caaagaaggt
     2821 agattggtcc aggagcatgt cgaagctctc ctgggtcagg ggaacaaagg acaggcccag
     2881 gagtttggac accgagccgg tggccaatcc cacgtctgct tctttagcca aaatggaaag
     2941 gcctatttca aagtgagtaa agacctcatt ttcatacccc ttgatcgatg aggagggaat
     3001 cttgaggtct ttcaggtgat ggtccaggag caccctcgtg ccagaaccct tctgccggtt
     3061 gacaaaccga attttgggcc gggtgaggtc cttgaaaccg cggacgtgca ggggattccc
     3121 gtgagccacc aggaaaccca ggtcccggtt gaatagattg accaccacgg tcttcaggtt
     3181 gggaagatag gtggacagaa aagggatgtt gtactggccg ctcttggggt ccaggagatg
     3241 ggaccaggcg atgtcggtga agccctggtt cagggctttc agcccgtcgg tgcttccggt
     3301 gttagccgag aagatgtaga actcgggata tgtcctgcgg atgtaggtct tcagaatatc
     3361 gaggatcgga tcgttactcc ccgctgccag aagggctcct tcaatgcgct tggttttttt
     3421 tcgggcctcg gccatccccg accgggcatt gttctcaatc cattcatcga tcaacctgcg
     3481 gggaaagacc catttccccg taacccgggt gcagggaatt cgtttggcct taatgagggc
     3541 gtacacctgc ttttcatgga ttcccagatg cttggcggcc tctttggtat tcatgagctc
     3601 ttcaggcatg gtcttccccc ttttatggct tgggttttta attatctttt ttaccgaatg
     3661 agtgaattct actacaaaat ataacttttt attaccaaaa aatttcccct tccgccaaga
     3721 taaaaatcga aaatgtgcta taatttttaa aagaattaca aatgaggagg taagcaatga
     3781 agatcagcgc tcggaatgtg ttgaagggaa aagtgaaaaa actggttcac ggagcggtaa
     3841 actctgaggt caccctcgaa atgcccggcg gaatgcaagt tgtctcgatc atcaccaaga
     3901 gttccgcccg aaatctgggg ctgaaaaagg ggaaaacggt ttatgcggtt atcaaagcct
     3961 ccaacgtgat gatcgccata gattgattcg gggcttgccc cggattgggg atccacgttg
     4021 cacccgttag aaaagacctc ccccagaacg gtcggaacct actttgccca taaattgagc
     4081 tgcttcgagg gaatcgcatc tagccagctt ccgctctccg gaaaatggcc tgttctgccc
     4141 caaaagtcca ggaatacccc gatgagccgg tgatttttct caaaggaaat tctggtccgg
     4201 catccctgac aacacttccc ctcgcttaac accgtctcgt ggtaggcgat ttcgatcgtt
     4261 ttgtaggtcg ccagacaata gcattgggcg caaaggattc tgtaccgatc ttcccggatg
     4321 tcataccatg cgcccaggga agttcccttg atgacccacg gcacgtaatg ggggggaatc
     4381 agttttgcga ttctgaggct acctt
//
LOCUS       DASPRR010000562         5315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_280981, whole genome shotgun sequence.
ACCESSION   DASPRR010000562 DASPRR010000000
VERSION     DASPRR010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280981"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1258
                     /locus_tag="VEL68_20700"
     CDS             341..1258
                     /locus_tag="VEL68_20700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ38460.1"
                     /translation="MARYLLRNFIQLILVCLGVATLVFVMVFLSGDPVMLMVPMTATP
                     EEIQNLRVAMGLDKPLWMQYGLYMSRAIQGDFGNSIKAELPAMRLFLERVPATLELGV
                     AGMVLATVVGVVVGILSAIRRDSWIDALGRILALGSQSMPVFWLALMLIILFSVQLKI
                     LPPFGRGGIKELIMPAITLATYNIPLILRLTRSAFFDVIHQDYIRTARAKGLPDRVII
                     YQHILRNAAIPILTVTGMNFGRILGGAIITETVFAWPGVGLLTVDAIFTADYPVIMAA
                     TVILVVSVVVVNFLTDLLYAVLDPRIRYD"
     gene            1333..2250
                     /locus_tag="VEL68_20705"
     CDS             1333..2250
                     /locus_tag="VEL68_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014278255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ38461.1"
                     /translation="MAAISGKDTKELKLLWWNLEGVRLPRSVRAIIRSKTAILGIIFV
                     LIALAAAIFAPYLSPHDPFKQNVTMRLKPPAWAEGGNSKFFLGTDQLGRCVLSRLIYG
                     ARISLIVAAVAVLIAAVLGVALGLIAGFFGGWVDQGIMGVTNFFLSFPYVLLAIAIMG
                     VIGPGFSTLILVLGLTSWPVYTRIIRSEVLSVKELEFVDAARALGVREDRILIRHILP
                     NVMNSVMVTASLETARMIINEAFFSFLGLGVQPPIPSWGNMLNDGKAYIFVTWWLVTF
                     PGVAIFLTTLGTNLIGDWLRDLFDPHMVF"
     gene            2601..3629
                     /locus_tag="VEL68_20710"
     CDS             2601..3629
                     /locus_tag="VEL68_20710"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXZ38462.1"
                     /translation="MEKLRGDLLELVQVPGPSGFEGPVAECIQKKIEPHVSRVEIDGM
                     GNLLAVKKGPPGSSVFVLMAHMDEVSMVVTYSGDGFIRFDFVGHINPAVVVGQPVKVL
                     TQSGPIPGVGCSPSVHLAQSVGDLWMDVGPRISQVEPGDPIIFNTTPYWLDEEEKVLA
                     SKAVDDRTGCAVLIETARRLENLDLGITLIFAFTVQEEVGARGAEYVARQIHPTWAVA
                     LDNAYASSPGLGPDKAVPLGSGPVIRRFESAKPGRGMAISFSDPDLVRALRAAGKAAN
                     LPIHVDTRFNLFTDASGAYEAFSDIKCTSLTIPRRYSHSPYEVTHLDSLQNTVALLCK
                     YFESNWKR"
     gene            3643..4647
                     /locus_tag="VEL68_20715"
     CDS             3643..4647
                     /locus_tag="VEL68_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38463.1"
                     /translation="MTGTLLKISNLKTQFFTGEGIIRAVDGVDLTISQGETLGVVGES
                     GSGKSVTAYSALNLISYPGRVVAGEVFFRGENLLEKPPEEMKEIQGNKISMVFQEPMT
                     SLNPVFTIGRQVMEPLIYHKNYSSADAHQEAVKLLKLVGIPSAETRIDEYPHQLSGGM
                     RQRVMIAMALACRPELLIADEPTTALDVTIQAQILTLMKRLQKEFGMAIMLITHNLGV
                     VAQMVDRVVVMYAGQVVETGGVERIFDQPAHPYTVGLLGSIPKLNERRERLQVIEGTV
                     PSSSQMPEGCRFHPRCSRVTEICRQELPSLAETEPGHWVRCWLYEKEIPSTKFQIPTI
                     "
     gene            4770..>5315
                     /locus_tag="VEL68_20720"
     CDS             4770..>5315
                     /locus_tag="VEL68_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ38464.1"
                     /translation="MSNYRPLLEVKNLVKHFPQKAGFLSRRSGIVRAVDGVSFTLDSG
                     ETLGLVGESGCGKTTTGRIVLSLLRPTSGEVIFQGRNVHFLKKEELRRLRKEMQIIFQ
                     DPYASLNPRMTVGQILGEALGIHHNLRGKAAKERMRELLEAVGMSAEHIPRFPHEFSG
                     GQRQRIGVARALAVQPKLIICD"
BASE COUNT         1132 a         1430 c         1569 g         1184 t
ORIGIN      
        1 gctttggttc tgctgggaat ctatctgtcg acacgggagg agaaaaaact gggaaaaatc
       61 tgaaccgtag cgcccggaac tagtacaaac gcattaaata atgtcacatg tcattaggag
      121 cccgaagggc gcggcaatct caccccctcc ccgccctccc ccctcggagg ctgtgtcgca
      181 actaagggga agggcgtcat tccggcgaaa gccggaatcc agtgttcggt tttattcaat
      241 ttatttgaat cctggatgcc ggcttttgcc ggcatgacga attacgacac agtctctcga
      301 gggggagggt ggggatgggg gggaaagcga aaagaagcct ctggcccggt accttcttcg
      361 aaatttcata cagctcatcc tggtctgtct cggggtagcg accctggtct tcgtcatggt
      421 ctttctgagc ggggacccgg tcatgctcat ggtccccatg accgccaccc cggaagaaat
      481 ccagaacctg cgtgtggcca tggggctgga taaacccctc tggatgcagt acggattgta
      541 catgtctcgg gcgatccagg gggatttcgg caactccatc aaggcggagc tgccggccat
      601 gcgcctcttc cttgaaaggg tgccggcaac cttggagctg ggggtcgccg gaatggtcct
      661 ggccacggtg gtcggggttg tagtggggat tctctccgcc atacggaggg atagctggat
      721 cgatgccctg ggccgcattc tggccctggg aagtcaatcc atgccggttt tttggctcgc
      781 tctcatgctc atcatccttt tttcggtcca attgaaaatc ctgccacctt tcggcagggg
      841 gggaatcaag gaattgatca tgccggccat caccctggct acctataata ttcccctgat
      901 cctgcgcctg acccgttccg ctttttttga tgtcatccat caggactaca tccggaccgc
      961 ccgggccaag gggctgccgg accgggtcat catttatcag cacattttgc gtaatgcggc
     1021 gatccccatt ctcacggtca caggcatgaa tttcggccgg atcctggggg gagcgatcat
     1081 caccgaaacg gtcttcgcct ggcccggtgt gggtctcctg accgtggacg ccattttcac
     1141 cgccgattac ccggtcatca tggcggctac ggtcatcctg gtggtgagcg tggtggtggt
     1201 gaattttttg accgatctcc tgtacgcggt cctggacccg cgaatacggt acgattgagc
     1261 aggacgtcat ccgtgtccac gcttccctga acggcgcgtt cgggaaacac gccctacagt
     1321 atagaaattt gcatggcagc catttccggt aaagatacaa aagagctgaa actcctctgg
     1381 tggaacttgg agggggtccg tctgccccgc tcggtgcggg ctataatccg gagcaagacc
     1441 gccatcctgg ggatcatctt cgtactcatt gcgctggcgg cagccatctt cgctccctac
     1501 ctatctcccc atgacccctt caagcaaaac gtaaccatga ggctgaaacc tccggcctgg
     1561 gctgaggggg gaaattcgaa attttttctg gggacagacc agctgggaag gtgcgtcctt
     1621 tcccgcttga tctacggggc gcgcatctct ctcatcgtgg cagcggtggc cgtgctgatt
     1681 gccgcagtgc tgggagtggc tctggggctc attgccggtt ttttcggcgg ttgggtcgac
     1741 cagggaatca tgggggtcac taactttttc ctgagctttc cctacgtctt gctggccatc
     1801 gccatcatgg gggtcatcgg gccgggattc agcaccctga tcctggtcct ggggctgacc
     1861 tcctggcctg tctatacccg gatcatccga tccgaagttc tgagcgtgaa agagctggaa
     1921 tttgtcgatg cggcccgggc gctcggggtg cgggaagacc ggattctcat ccgccacatc
     1981 ctgcccaacg tgatgaactc agtcatggtc acggccagcc tggaaacggc ccggatgatc
     2041 ataaatgaag ccttcttcag cttcctgggc ctgggggtcc aaccccccat tccctcctgg
     2101 ggaaatatgc tcaacgacgg gaaggcttac atcttcgtga cctggtggct ggtcaccttt
     2161 cccggagtgg ccatcttcct gacgacgctg ggaacgaacc tgattgggga ctggctgagg
     2221 gacctgtttg atccacatat ggtcttttaa aatgcggaat gcgaaattca gaatgcggaa
     2281 tggaagcggg gaagaatgga ttggaaaata agtcatcgaa acggagaata ttcaacagag
     2341 gaattttgaa aaaggaattt gattaagagc ttatctttga ataagggtct gtctttaaat
     2401 ccccctttga aaaaggggga cagaggggga tttgctttag atcgatttga aaaaatcccc
     2461 caaacccccc ctttacaaag gggggggatt attatttacg gacaagttct aagaagggat
     2521 tgcgaatgga aaatgcgaat tgccgccacc agctatcttt attccgcatt tcgaattccg
     2581 cattctaagg ggggaatggc atggaaaaac ttcgcggtga tttgttggaa ctcgttcagg
     2641 tccccggtcc ttctggcttc gaagggccgg tggccgaatg catccagaaa aagattgagc
     2701 cccatgtttc ccgggtggag atagacggga tgggaaacct cctggccgtc aagaaggggc
     2761 ctccgggatc atccgtattt gtcctcatgg cccacatgga tgaagtgagc atggtggtga
     2821 cttacagcgg cgacgggttt atccgcttcg attttgtggg tcacattaat ccggccgtag
     2881 tggtcggcca gccggtgaaa gtattgaccc aatccggccc catcccgggg gtgggctgct
     2941 ctcccagcgt gcatttggct caaagcgtgg gagatctatg gatggatgta ggtccgcgca
     3001 tcagccaggt ggagcctgga gaccccatta ttttcaacac cactccgtac tggttggatg
     3061 aggaagaaaa agtcctggcc agtaaagcag ttgacgaccg gacgggctgc gcggttctca
     3121 tcgaaacggc ccggcggctg gaaaatctgg acctggggat caccctcatc tttgccttta
     3181 ccgttcagga ggaggtaggc gcacgagggg cggagtatgt ggcccgccag attcacccta
     3241 cctgggcagt ggccctggat aacgcgtacg cttccagccc tggtttgggg ccggataaag
     3301 ccgtgccttt ggggagcggg ccggtcatcc gacgttttga gtcggccaaa cccggaaggg
     3361 gaatggccat cagctttagc gatccggacc tagtgcgggc gctgcgtgcc gcagggaagg
     3421 cggcgaacct gcccatccac gtggataccc ggttcaacct cttcaccgat gcttccggcg
     3481 cctatgaagc tttctcggac atcaaatgca cttcccttac catcccgcgg cgctactccc
     3541 attcgcctta cgaggtgact catctggatt cgctgcagaa cacggtagcc ctgctgtgca
     3601 agtactttga gtcgaactgg aagagatagg aaagaaaaga ctttgaccgg aacgctgctc
     3661 aagatcagca acctgaagac ccaatttttc accggagagg ggatcatccg cgcggtggac
     3721 ggcgtggacc tcaccataag ccagggagaa acccttgggg tggtcggaga gtccggttcg
     3781 ggcaagagcg tgaccgccta ctcggccctc aacctcatct cttatccggg ccgggtggtc
     3841 gcgggagagg ttttcttccg gggggagaac ctgctggaaa agccaccgga ggagatgaag
     3901 gagattcagg ggaacaagat ctcaatggtt ttccaggagc ccatgaccag cctgaatccg
     3961 gtcttcacca tcgggcggca ggtcatggag cctttgatct accataagaa ttactccagt
     4021 gccgatgcgc accaggaggc tgtcaaactc ctgaaactgg tgggaatccc ctcggccgag
     4081 acccgcatcg atgaatatcc tcatcaactc tccgggggga tgcgccagcg ggtcatgatc
     4141 gccatggccc tggcctgccg gcctgaactg ctgatcgccg acgaacccac aaccgctctg
     4201 gacgtgacca tccaagccca aatcctgacc ctcatgaaga ggctgcagaa ggagttcggg
     4261 atggccatca tgctcatcac tcataacctg ggagtggttg cccagatggt cgaccgggtg
     4321 gtggtcatgt acgccggtca ggtagtggaa acaggtggag tggaaagaat ctttgatcag
     4381 ccggctcacc cttacactgt tggcttgctc gggtcgatcc ccaaactgaa cgagaggaga
     4441 gagcggcttc aggtaatcga gggaacggtc cccagttcaa gtcagatgcc cgaaggctgc
     4501 cggttccatc cgcggtgttc ccgggtgacg gaaatatgcc gccaggaact cccttccctg
     4561 gccgagaccg agccgggaca ttgggtgcga tgctggctgt acgaaaagga aatcccaagc
     4621 accaaattcc aaattccaac gatctaaacc ttcttgtttg gaatttgcga tttggaaatt
     4681 ggggtttatt tgggatttgg tgcttgagat ttgggttttg aatctcatca atggatgggt
     4741 taaatttcac ccaagggaat ccggtagctg tgagtaatta tcggcccctt ctggaagtca
     4801 agaacttagt caagcatttt ccccagaagg cgggctttct ttcccgccgg tcggggatcg
     4861 tccgtgcggt ggacggggtg agcttcaccc tggactccgg cgaaacgctg ggcttggtgg
     4921 gagagtccgg ttgcgggaaa actaccaccg gccgaattgt tctctccctt cttaggccca
     4981 cttccggcga agtgattttc cagggccgga atgtgcactt cctaaagaaa gaggagcttc
     5041 gacggctgcg caaagaaatg cagatcatct tccaggaccc ctatgcttcc ttgaaccccc
     5101 gcatgacggt cgggcagatt ctgggagaag ccctgggaat tcatcacaac ctcagaggaa
     5161 aggcggccaa agagcgaatg agggaactgc tggaagccgt gggaatgagc gccgagcaca
     5221 tcccccgttt tccccatgag ttcagcggag gacagcgaca gcggatcggg gtggctcggg
     5281 cccttgccgt gcagcccaag ctgatcatct gcgac
//
LOCUS       DASPRR010000563        13440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1264250, whole genome shotgun sequence.
ACCESSION   DASPRR010000563 DASPRR010000000
VERSION     DASPRR010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264250"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VEL68_20725"
     CDS             complement(<1..383)
                     /locus_tag="VEL68_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXZ38465.1"
                     /translation="MEIKKICVLGAGVMGSGIAQVSAETGYQVSMRDVEDRFVRGGIS
                     TIKKNLERAISKGKMTKENAEGILSKINGLVDLPAAAKDADIVIEAITENMDMKKQVY
                     GDIDLLCPKETILASNTSGLSITEIA"
     gene            complement(820..1506)
                     /locus_tag="VEL68_20730"
     CDS             complement(820..1506)
                     /locus_tag="VEL68_20730"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HXZ38466.1"
                     /translation="MITAKTFRVGLLVPSSNTVMEVDLYRNLPSSITIHPARMYLEET
                     TPQGEERMISEELPRAAQLLRTLHPHRVVFGCTSAGALHGASFPQEINRTIGPIVGCP
                     VLDILSPVAEELERFGAKKLAVLTPYASALNQSIQAGLEALGFQVLSIDGMGITANFE
                     LATPTPEEIFAFVQNSKIPQNAEALFISCTNFRALEARSLMREHFRLPIVTSNQAIIE
                     KIKRIYLEGA"
     gene            complement(1571..2443)
                     /locus_tag="VEL68_20735"
     CDS             complement(1571..2443)
                     /locus_tag="VEL68_20735"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HXZ38467.1"
                     /translation="MDKRMEVLEGAIDLHFHATPDLHDRLLDEVEIAKEAREVGMRAV
                     LSKAHYGINASRMYYVSRAVPGIHTFGGVVLNPAVGGLNPSAVEAAIAFGGKEVWMPS
                     IFSAAHIKHFKGTYGTMPGRVKWPEKGISILGSEGKLLPAVKEILDMIAQANIILGTS
                     HCSAEESRILIDEALRRGVKKILVTHPHNEVPSLSLEVQLEFARKGAYLEHCFLPTTP
                     MFFNARFQEVIEVIRKAGPQQCVLATDFGQVFMPSPVEGLRMFILSLMNQGITREEID
                     IMVRKNPAKLLGLD"
     gene            complement(2448..3593)
                     /locus_tag="VEL68_20740"
     CDS             complement(2448..3593)
                     /locus_tag="VEL68_20740"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXZ38468.1"
                     /translation="MTDTFNFDEIVNPQLKKIVPYLPGPLTKDIAARYEMDPARVIKL
                     SSNENPLGVPPLARAKIIEMAGEASLYPDAEARELRGALAQYVNMRPENIVVGPGSSS
                     LMMHIIEVFSGPGDEIVFTSPAFGSFYEEIALARGRTPVKVHLEENFDLDVDKLKKAI
                     TRRSRVVFITRPNNPTSKMVPLKDLREIMGAAAAVKAVVVSDEAYVEFADPHTSALEF
                     FYEMGEGINLLVTRTFSKVFGLADVRLGYCVGSVEAISFLTKAKPKWGTGMLAQIAGV
                     AALKDKEHFQRTLEVVRKGREFLHRELGRLGLKMVDAPQGNYVTAKVSEFGYRAEDFT
                     EELCRRGGIMIRGDFHPEYVRISIGTMPQNEALIKTVAEMIKDRKRR"
     gene            complement(3815..4528)
                     /locus_tag="VEL68_20745"
     CDS             complement(3815..4528)
                     /locus_tag="VEL68_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38469.1"
                     /translation="MPQVEIQDLRVAYGKAKILEGISMEVEPRELVSVIGPNGAGKTT
                     LLRAISRVAESQGSIYFEGDRVDHLSPVEVVRRGIIHCPERRRLFPDLSVQANLEMGA
                     FLRHKDKEGVQKDMGKMFNIFPILQERRTQDARTLSGGEQQMLAIARSLMSQPKFLML
                     DEPSMGLAVLVKRFLAETIKGIQKNGVTVLLVEQDADLAFSVADRVYVLEHGKVALQG
                     LPDNLMKNPHVKEIYLGIA"
     gene            complement(4515..5267)
                     /locus_tag="VEL68_20750"
     CDS             complement(4515..5267)
                     /locus_tag="VEL68_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38470.1"
                     /translation="MGIILETQELTKYFGGLGAVRKVSFTLIQGEMLGVIGPNGAGKT
                     TLFNLITGFLKPTEGNISFRGERITGLKPHEIVRKGIARTFQIVRPFHQLTALENVII
                     SCLSPREGGQGKKDGRVEEKAAKLMESVGLSGKEGMLAKDLPHGDLKRLDLARALATE
                     PEVLLLDEPFAGLGLMEISSLSPLIQQLSKGGHSIIIIEHKLREMMKLVQRVITLNFG
                     EKIADGTPEEISRDEKVIQAYLGKGGMTLAAG"
     gene            complement(5275..6231)
                     /locus_tag="VEL68_20755"
     CDS             complement(5275..6231)
                     /locus_tag="VEL68_20755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38471.1"
                     /translation="MPEQNRRSTFFGERVGLLIALAVLSAVPLFVKNRAIVEIIILAN
                     IYAVYVASWDILCGYTGQVSFGHALFIGGGAYTAALLNFYFKFPPWVTIFLGGGVAAL
                     FGLIIGTPCLRLRGPYFALATFAAAAFPYGLTDVFWEVTGGLDGLYGIAPIWPTLEGK
                     YYLSVLLTAICCGLVYLGGRSKKGLILKSIREDEVAAMASGVNTTVYKLTTFVISGFL
                     AGVAGAFYSHNQLHVGPETLSINLSVLVIIMSVVGGIGTITGPIVGAYLLTIFNEMMR
                     EWEELRLLIYTASVVLFLLFLPKGVLPTLTDGIKQWLKRKGG"
     gene            complement(6246..7112)
                     /locus_tag="VEL68_20760"
     CDS             complement(6246..7112)
                     /locus_tag="VEL68_20760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38472.1"
                     /translation="MNIFLEILVYGAVQSSIYALLAMGFCLIFGVANIVNLAHTALYM
                     IGAYLIYSFFSVLRLNLAMAILLSVVSTGLLATGMYRYCIRPVILSEWSVMMITISLA
                     MFFQQLMIFIYGPADRNVQGFIEEKLALFGMVDIDAQRLLTLVVSVILLVLLWLFIYR
                     TRMGKAILAVAQDREAALFMGINSERVYMAVIAISAVLAAVSGAFIAPTIGARPEMWA
                     PTLGKIFAVVVLGGIGSLEGTILAALLIGYLEVIISFSISSYFGEIVSVIVILLMLTF
                     RPSGLLGKRIEA"
     gene            complement(7263..8477)
                     /locus_tag="VEL68_20765"
     CDS             complement(7263..8477)
                     /locus_tag="VEL68_20765"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ38473.1"
                     /translation="MGRGLKLSEEKTIRVGIAKGKDVVKAVRQAVSLAGGLDQRISPD
                     SRVLIKPNLSVALPARSGVTTDPRVVIALIHLVREASAREILVGESAVVGFDAGEIFD
                     ALKVRSLFEKAGARVVNLDQDQPVEVKVPRGEVLKKVKIWRTAYESDVIISVPKMKTH
                     FQTGVTLSLKNMKGTVTDESKRIIHRIGVPVKKQEEYGLDQGIVDLNTVISADLAVID
                     ATVSLEGFVPGPRLVGNPVRMDTIIAGFDPVAADAVGCRVIGLDPREVRHIRLAYERK
                     MGRMLPQEIEVKGRSIQSVLHPLKTEVPEVAQVHKNITIIEGRGCSGCTVTNCMALSF
                     YSPEAAESLGRVTLVVGDTTSKDYRGEGQCFFIGNCAIRSNKGKDGVRIGGCPPPGVW
                     IRRSINEAGKEK"
     gene            complement(8667..9056)
                     /locus_tag="VEL68_20770"
     CDS             complement(8667..9056)
                     /locus_tag="VEL68_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXZ38474.1"
                     /translation="MKKEMIHSEKIPKPVGPYSQGIKVGNLVFTAGTGPFDLKTGQLL
                     LGDIRHQTRQVLENIKAILDAAGARMEDIVKTTIFLTDLKDWSAMNEVYREYFRQDPP
                     VRSTVQVAALWGGMAVEIEAIAALSER"
     gene            complement(9170..10387)
                     /locus_tag="VEL68_20775"
     CDS             complement(9170..10387)
                     /locus_tag="VEL68_20775"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38475.1"
                     /translation="MGRKVFIFCAVGFLVLGVSAQIYAQGKVIKMGVTAALQKEAGIS
                     VKYAAEMAAEEINAAGGILGNKVELSFADDDGIAEKGVTAIKKLLFTDKADFISGGWM
                     SGVGLAQAPHIFEAKKLWLSGGPATPKLAKMVEENYNQGKYFFRVGCVNSDLFAYDMA
                     IFAEELFKKELKITKVALLPESSVWAREMAAFLEKELPKRGLKIVYTDVFDPKRTDFS
                     PQFAQIRAKGAEILLTVQALSPGVPITNQWTDTKLPVHQAGYSLNSQVSDFWDKTGGK
                     CQYESTQLINGARAPISPKTISFYDKYVSTYKLSPTYTGFAEYDALYLLKAAAEEAKS
                     LDTEVLIKTLEKMRYVGVAGVIAFDKNHELKYGPEGKQPVWAQWQEGKQVVLFPTQWA
                     TGKYISPPWIKKK"
     gene            complement(10490..12421)
                     /locus_tag="VEL68_20780"
     CDS             complement(10490..12421)
                     /locus_tag="VEL68_20780"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ38476.1"
                     /translation="MARYETLFSSFIQGGLKLKNRIVMAALNNNFSNEDGTANDRIAA
                     FLKERAEGGAGLIISEACPVSSKGRHRLRCICAHEDAFLPSLQRLARVVHDCGSTLAL
                     QLHHAGRIASPEIIGGPPLAPSPIPRGPGYPIPREITREEIQETVVDFGAAARRAKEA
                     GFDAVEVHGAHGYLIHQFLSPTTNQRKDAYGGSSENRLRFSLEIVREVRKQVGRTYPV
                     LFRLSAQEYIPGGYELEEVLDWVAEWERAGVSVIDVSGGTNETLRSSLHAMPPMFFPR
                     GYHVPLAAAIKKMVRLPVVAVGRLDSPEFMEQVLKEGQADLVAVGRPFLSDPHWPAKA
                     ARGEEDRIRPCVYCNHCIWELFQQKDITCFQNAAVGREKECQIHPAKKVKDVFVFGGG
                     PGGMEAARVAKKRGHRVTLFEKSPRLGGQMILAAALPRKQNYFKSMDWLIGEIQREGV
                     DVRVSSEGNLAVIDKEKPDAVIVATGASPAVRLPRASSSILTAWEVLAGKEVGKRVLI
                     SGGGMVGIETAEFLSERGCQVTVITSREGPEQLATDMEGTTRALLLERLPASKISFLF
                     AAQVLELGQGRVLVTQGGKRQWLEAETIISAQGSRSEQDLLRALGGKIPQILPIGDCV
                     EPRSAKEAIHEGFLAGLKV"
     gene            complement(12574..13320)
                     /locus_tag="VEL68_20785"
     CDS             complement(12574..13320)
                     /locus_tag="VEL68_20785"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HXZ38477.1"
                     /translation="MKKAGRSSKKKGGERSAIAEKVIGIIGGMGPEATIDLFQKIVKA
                     TPARTDQEHLRILIDCNPKIPDRVKAIFEQAESPVGGLIAAAQNLERSGAGLLLIPCN
                     AAHYYHGEVAKKVAVPVLHMIEETARYCRRKFPRLETFGLLAGSSTVVLGLYSKAFAK
                     VRKKILNPRLETQDKIMNCIYEIKAGNLGAQVRAELLRAAQELAEAGAQAILLGCTEI
                     PLVLQDGDLPLPFIDPAQVVAEISIALARQ"
BASE COUNT         2955 a         3939 c         3413 g         3133 t
ORIGIN      
        1 gcgatctcgg taatggaaag gcccgaagta ttggaggcca agattgtctc tttggggcaa
       61 aggaggtcga tgtcaccata gacctgcttc ttcatgtcca tgttctctgt gatggcctcg
      121 attacgatat ccgcgtcttt agcggcggca ggcaggtcca caagcccatt aattttggag
      181 agaattcctt cggcattctc cttcgtcatc ttccccttgc tgatcgccct ttccaaattc
      241 tttttgatcg tgcttattcc acctcgtacg aagcggtcct cgacatcccg catggagact
      301 tgatacccgg tctcggccga cacctgggca attcctgaac ccatcactcc tgcccccagg
      361 acgcatatct ttttgatctc cacgatttct ccctcccaaa tttcagaatc cgattcgttg
      421 caccgaatgc aaaagaaata aataagttgt aattgcgaga agcacagcga cgaaacaatc
      481 tcaaacaaag gcgagattga cgcgccctcc gggctcgcaa tgactttaaa agtggagagg
      541 cccttgcaaa agtcacttta tccgtcattc cggcgaaagc cggaatccag aatggcttga
      601 aaatcctgga tcccgggtcg cgcttcgctt gcccggaatg acgatttcct cttgatttaa
      661 gagttttgcg agaggctcag tattcactcc gtttgtttga aataaatctt ccgcaaagca
      721 tggataaatt tttcatctaa actcccgcat gaaaagtcca gttaagactc cgggcagctg
      781 atgacctgag caacctcgtt tttttgaagc ttaaaacgct taggctcctt ccagatagat
      841 tctctttatt ttttcgatga tggcctggtt actggtaacg atgggaaggc ggaagtgttc
      901 ccgcattagg gacctggcct ccaaagccct aaaattggtg caggagatga acaaggcctc
      961 agcgttctgg gggattttgc tgttttggac gaaagcaaag atttcttcgg gagtgggagt
     1021 ggccagttca aaattcgctg tgatgcccat tccgtcgatg gaaagaacct gaaacccgag
     1081 ggcttccagg ccagcctgga tggactgatt cagcgcggaa gcataagggg tcagaacagc
     1141 cagttttttg gccccgaatc gctccaattc ctctgccacg ggtgacagaa tatccaggac
     1201 agggcagccg acaataggtc cgatcgtacg gttgatctcc tgtggaaagc ttgccccatg
     1261 aagagctccg gctgaagtgc aaccgaagac gacccggtga ggatggagag tccgaagcaa
     1321 ctgagcggcc cgcgggagtt cctcagaaat catccgttcc tctccctggg gagtagtttc
     1381 ttccagatac atccgggctg gatggatggt aatcgaagag ggaaggtttc gataaaggtc
     1441 cacctccatg accgtgttgg aagagggcac caacaggccc acccggaagg ttttggcggt
     1501 gatcatcttg tatctcctcg ggcgcaggaa gcctccaagg cagaaatcct cccccaaatg
     1561 cccgtttatt tcaatccagg cccaacagtt tggctggatt tttgcggacc atgatatcga
     1621 tctcttcccg ggtaatcccc tggttcatca aggagaggat gaacatccgc aggccttcca
     1681 ccggagaggg catgaagact tggccgaaat ccgtggccag cacgcactgc tgcggcccag
     1741 cctttcggat gacctcgatt acctcttgga accgggcatt gaaaaacata ggggtggtag
     1801 gcaggaagca atgctccagg taagctcctt tccgggcaaa ttccagttgc acttccaaag
     1861 aaaggctggg gacttcatta tgcggatggg tcaccaggat tttcttcacg cccctccgga
     1921 gggcttcgtc gatcagaatc cggctctctt cagccgaaca gtgagaagtt cccaggatga
     1981 tattggcttg ggcgatcata tccaggatct cttttaccgc gggcagaagc tttccttcgg
     2041 agcccagtat ggagatccct ttttctggcc acttcacccg gcccggcatg gtgccatagg
     2101 tccccttgaa atgcttgata tgggcggccg aaaagatcga aggcatccac acctcttttc
     2161 ccccgaaggc gatggccgcc tccactgcgc tgggattgag tccccccacg gcgggattca
     2221 ggactacccc cccgaaggta tgaattccgg gtacggctct gcttacgtaa tacatccggc
     2281 tggcattgat cccgtaatgg gctttggaaa gcaccgccct catcccgacc tccctggcct
     2341 ccttggcgat ctccacttca tccagcaacc gatcgtggag gtcgggggtg gcatggaaat
     2401 gaaggtcgat cgccccttcc aaaacttcca ttcgtttatc cattttttta cctccttttt
     2461 cgatccttga tcatttcagc tacggtcttg atcagcgctt cgttctgggg catcgttccg
     2521 atactgatcc gaacgtattc ggggtggaaa tcaccccgaa tcatgatgcc tccccggcgg
     2581 cagagctctt cggtgaaatc ctccgctcta tagccaaact cggagacttt ggcagtgaca
     2641 tagtttccct ggggtgcgtc caccatcttc aagcccaggc ggccaagttc gcggtgcagg
     2701 aattccctcc cttttcgcac cacctccaag gttcgctgga aatgctcttt atctttcaag
     2761 gccgctaccc ctgcaatttg agccagcatc cccgttcccc acttgggttt ggccttggtc
     2821 aggaaagaaa tggcctccac agaacctacg cagtaaccca accggacatc ggccaggcca
     2881 aagactttgg aaaaggtccg ggtcaccagc aggttgatcc cctcgcccat ttcgtagaaa
     2941 aattccagcg ccgaagtgtg aggatcggca aattcgacgt aggcctcatc agaaaccacc
     3001 acggccttca ccgcggccgc cgcccccata atctcccgca ggtccttcag tgggaccatc
     3061 ttgctggtgg gattgttggg acgagtgatg aagacgaccc ggctcctccg ggtgatggct
     3121 tttttcagct tgtccacatc cagatcaaag ttctcctcca gatgtacctt aacgggggtt
     3181 cgtccccggg ccaaggcgat ctcttcgtag aagctcccga aggccggaga ggtaaaaacg
     3241 atctcatctc ccgggccgga aaagacttcg ataatgtgca tcatcagaga agaagacccc
     3301 gggccgacca caatattctc cggccgcatg ttcacgtatt gtgccaaagc tcccctcaac
     3361 tcgcgggcct ccgcatccgg atagaggctg gcttcaccgg ccatctcgat gatcttggct
     3421 ctggccagag gtggaacgcc caaggggttc tcgttggagg agagcttgat cacccgggcc
     3481 gggtccattt cataccttgc cgcaatgtcc ttagtcagcg ggcctgggag gtaggggaca
     3541 atttttttca actgggggtt gacgatttca tcaaagttga atgtatcggt catcgattcc
     3601 ctcctttaat taatatgttc catttttttc gattcgcccc ttttttggtt ttccttcggc
     3661 tttctccgcg gcttcgttac taccaacgtt caatatcccc cccaccctaa ccgttcggct
     3721 gagctcacga cgaagccctc ccccctcgga gggaggggcg gaagggggtg atttaattgc
     3781 ggcgcagccg cgctgtgatg aatcgatcca ctttttaggc aatccccaag tagatttctt
     3841 taacgtgggg attcttcatc aaattatcgg gtaaaccttg gagagccact ttcccatgtt
     3901 ccagaacata gactcggtcc gccacgctga aagccaaatc agcgtcctgt tccaccagaa
     3961 ggacagtcac tccgtttttt tgaatccctt tgatcgtttc tgctagaaat ctcttgacca
     4021 agaccgctag tcccatggag ggctcgtcta gcatgaggaa cttgggctgg gacatgaggg
     4081 atcgggcgat ggccagcatt tgctgctctc cgccgctcaa cgttctggca tcctgggtgc
     4141 gcctctcctg gaggatgggg aaaatgttga acatctttcc catgtctttc tgaacccctt
     4201 ctttgtcttt atggcgcaga aaggccccca tctccaggtt ggcttggacg ctcaggtcgg
     4261 ggaacaggcg cctgcgttcc gggcagtgga tgattccccg cctcaccact tccaccgggg
     4321 aaaggtgatc aaccctatcc ccttcaaaat atatggaacc ttgcgactcg gccaccctgg
     4381 atatagccct cagcaaggtg gtctttcccg caccattggg gccgatgacc gacacgagct
     4441 cccgtggctc cacttccatg gagatccctt ccaatatctt ggctttcccg taagcgaccc
     4501 gaaggtcctg aatttcaacc tgcggcaaga gtcatccccc cctttcccaa ataggcttgg
     4561 attacttttt cgtcgcggga tatttcctcc ggggttccat cggcaatctt ctccccgaaa
     4621 ttcagagtaa tcaccctttg gaccaatttc atcatctccc gaagcttgtg ctcgatgatg
     4681 atgatggagt gtcccccctt ggataattgt tgaatgaggg gagaaaggga ggagatctcc
     4741 attaatccca acccggcaaa gggttcatcc aacaagagga cctccggctc cgtcgccaga
     4801 gcccgggcta aatccaggcg tttcaggtcc ccatggggca aatccttggc cagcatgccc
     4861 tccttgcccg acaagcccac gctctccatg agcttcgcgg ctttttcctc gacccttcca
     4921 tcctttttcc cctggcctcc ttcccggggt gaaaggcagg agatgatgac attctccagg
     4981 gcggtgagct ggtgaaaggg cctcacgatc tggaaggttc gagcaatccc tttgcgcacg
     5041 atttcatgcg gtttcaggcc cgtaatcctt tctccccgga aggaaatatt tccctccgtt
     5101 ggtttgagaa agccggtgat caaattgaag agggtagtct tcccggctcc gttaggccca
     5161 atgaccccca gcatttcccc ctggatgagg gtgaagctca ccttgcggac cgctcccaac
     5221 cccccaaagt acttggtcaa ttcctgggtc tccaaaatga tgcccataca ggcttcaccc
     5281 cccctttctt ttgagccact gtttgatccc atccgtgagg gtgggcaata cccctttggg
     5341 aaggaacagg agaaaaagca ccaccgacgc agtgtaaatc agaagtcgca actcttccca
     5401 ttcccgcatc atctcgttga agatggtcaa gagataagct ccaacgatgg gcccggtgat
     5461 cgttccgatt ccccccacca cgctcatgat gatgaccaag accgacaggt tgatggacaa
     5521 ggtctccggc cccacgtgga gctggttgtg ggaatagaag gctcccgcca ccccggccaa
     5581 gaacccgctg atcacgaagg tggtcagctt atacacggtc gtattgacgc ccgaggccat
     5641 ggcggcaacc tcgtcttccc gaatcgattt taagatcaac ccctttttcg accggcctcc
     5701 caggtacacg agaccgcagc atatcgcggt cagaaggacc gaaaggtaat actttccctc
     5761 caaggttggc cagatgggag cgattccata gagaccgtcg aggccgccgg tgacttccca
     5821 aaatacatcg gtcagcccgt agggaaaggc ggccgcggca aaggtggcca gggcaaaata
     5881 gggtccccgg aggcgaaggc agggcgttcc gatgatgagc ccaaaaagag cggccactcc
     5941 tcccccgaga aagatggtca cccagggggg gaatttgaaa tagaaattca aaagagctgc
     6001 ggtgtaagcc cccccaccga tgaaaagggc gtggccaaag ctgacctgcc ccgtgtaccc
     6061 gcagaggata tcccaactgg ccacgtaaac cgcataaatg ttggctagaa tgatgatttc
     6121 cacgatggcc cggttcttga cgaaaagggg cacagcggaa agaaccgcca gggcaatgag
     6181 caatcccact cgttcgccaa aaaaagtgga gcgcctattt tgttcaggca tattttttcc
     6241 cctcttcaag cctcaatccg tttgcccaat aaaccagagg gacggaaagt aagcattagg
     6301 agaatgacga tcaccgacac gatttcgccg aaatagctgg agatggaaaa ggagataatc
     6361 acttccaaat agccgatcaa aagagcggcc aggatggtcc cttccaggct gccgattcct
     6421 cccaggacca ctacggcaaa gattttcccc aacgtgggag cccacatttc cgggcgagcg
     6481 cctatggtgg gggcgatgaa ggctccggag acggcggcca ggaccgcgga aatggcaatg
     6541 accgccatat agaccctctc gctgttgatg cccatgaaga gggccgcttc ccggtcctga
     6601 gccacggcca ggatcgcctt ccccatccgg gtccggtaga tgaacagcca gagaagaacc
     6661 agaagaatga ccgaaacgac gagagttaaa agcctctggg cgtcaatatc caccatcccg
     6721 aaaagggcca gcttttcctc gatgaaaccc tggacattcc ggtccgcagg gccatagatg
     6781 aagatcatca actgctggaa gaacatggct aaagagatgg tgatcatcat caccgaccat
     6841 tcggataaaa tgaccgggcg gatacagtaa cgatacatcc ccgtggccaa tagcccggta
     6901 ctcaccacgg acaggaggat ggccatggcc agattcaatc gaagaacaga gaaaaacgaa
     6961 taaatcaggt aggctccgat catgtacaag gcggtatggg ccaggttcac aatattagcc
     7021 accccaaaga tcagacagaa acccatggcc agaagggcgt agatgctgct ttgaacggct
     7081 ccataaacca aaatctctaa gaagatattc attgcttcga cctctcctgg aaaaagaatt
     7141 tatggctcat tctattcccc agatggattc ttacaccttt cacccccacc ctcaccctcc
     7201 cccgtcaaag ggggagggaa ccagggggga attttcaaat atctttggtc aggattttcc
     7261 ttctattttt cctttcctgc ctcgtttatg gaacgacgaa tccagacccc gggaggggga
     7321 catcccccga tcctcacccc atcctttccc ttgttcgatc ggatggcgca gtttccgatg
     7381 aagaagcact ggccttcccc tcggtagtcc ttggaggtcg tatcccctac tacgagggta
     7441 acccgtccca ggctctccgc agcctccggt gaatagaaag aaagggccat gcagttggta
     7501 accgtgcatc cgctgcatcc cctcccttcg atgatggtga tgtttttatg gacttgggcg
     7561 acctccggga cctccgtctt gaggggatgg agaacggatt gaatggatcg gcctttcacc
     7621 tcgatctcct ggggcagcat ccgccccatt tttcgctcat aggccaagcg aatgtgccgc
     7681 acctcccggg ggtctaatcc aatgactcga caacctaccg catcggccgc aacgggatca
     7741 aagccggcaa tgatggtatc catgcggacc gggtttccca ccaggcgggg gccgggaaca
     7801 aaaccttcca gggagaccgt ggcatcgatc accgctaggt ctgccgagat gaccgtgttc
     7861 aagtcgacaa tcccttggtc caaaccgtac tcttcctgtt tcttcaccgg aacaccgatg
     7921 cgatggatga tccgcttgct ctcatcggta accgtaccct tcatgttttt caggctcagc
     7981 gtgaccccgg tctgaaaatg ggtcttcatc ttggggacgc tgataatcac gtcgctttca
     8041 taggccgtgc gccatatctt gacctttttg agaacctcac cccggggaac cttcacctca
     8101 acgggttggt cctggtccaa gttgactacc cgggctccgg ccttctcgaa aaggcttcgc
     8161 acctttaagg catcgaatat ctctccggca tcgaagccga ccaccgcgct ctcccccacc
     8221 aggatttccc tggcgctggc ttcgcggacc agatggatca atgcgatcac cacccggggg
     8281 tcggtggtga ccccggatcg ggccgggagg gctacactca ggttgggttt gatgagaaca
     8341 cgggaatcgg gagagatgcg ctgatccaac ccgccggcaa gggagacggc ttggcggacc
     8401 gctttgacca cgtcttttcc tttggcaatt cccacccgga tggtcttttc ttcggataat
     8461 tttaaccccc gacccatggt cctgcctgag tctcctttga ataagagcct gtccgtaaat
     8521 aatacttccc cccttttgta aaggggggta ggggggattt tcccaggcca tctttaatca
     8581 aatcccccta tgtccccctt tttcaaaggg ggatttttaa agagcaatat atattaagga
     8641 taagctctaa gcaaatgcac cgatttttac ctctcggaaa gcgccgcgat ggcctcgatc
     8701 tccaccgcca tccctcccca gagcgcggcg acctgaacgg tggaacggac gggcgggtcc
     8761 tggcggaaat actcccggta aacctcattc atggccgacc aatccttcaa gtcggtgaga
     8821 aagatcgtgg ttttaacgat gtcctccatc cgcgctcccg cggcatcgag gatggccttg
     8881 atgttctcca aaacttgtcg ggtctgatgg cgaatgtctc cgaggaggag ctggcctgtt
     8941 ttcaaatcga aaggtcctgt gccggctgta aaaaccaggt tccccacctt gatgccctgg
     9001 gaataggggc ccacgggctt gggaattttt tccgaatgga tcatttcttt cttcatgacc
     9061 ggggcccctc cttttttcca tccatcgatt gtttcgattt cttacctccc ccggggccag
     9121 aaagagggaa aatcctcctc ctggcctttt gcctcccctt tccggattct tatttcttct
     9181 tgatccaggg gggagaaatg tatttgccgg tggcccattg cgtgggaaag agcaccacct
     9241 gcttgccttc ctgccactga gcccagacgg gttgcttacc ctccggtcca tatttcaact
     9301 catggttttt gtcgaaggcg atgactccgg cgactcccac ataccgcatc ttctccaggg
     9361 tcttaatcag gacctcagta tccagcgatt tggcctcctc tgccgctgcc ttcagcaggt
     9421 agagggcatc atattccgcg aagccggtat aggtaggaga cagtttgtag gtgctgacat
     9481 acttgtcata aaaggaaatc gtcttcggag agatgggggc tctggctccg ttgattaatt
     9541 gagtactttc atattgacat ttgccgcctg tcttgtccca gaaatccgaa acctgactat
     9601 tcaggctgta gcccgcctga tgcacgggaa gcttggtgtc ggtccattgg ttcgtaatgg
     9661 gaaccccggg agaaagcgcc tggaccgtga gcaggatctc cgctcccttt gcccgaatct
     9721 gggcaaactg gggggagaag tcggtcctct tgggatcgaa gacatccgta tatacgatct
     9781 tcaacccccg cttgggcagc tccttttcta aaaaggcagc catctcccgg gcccatacag
     9841 aactctctgg gagaagagct actttggtta ttttcagctc cttcttgaag agctcttccg
     9901 caaatatcgc catgtcatag gcgaagaggt cggaatttac gcaaccgaca cggaagaagt
     9961 acttgccctg gttatagttc tcctctacca tcttggccaa tttgggggtt gcgggtccgc
    10021 cgctcagcca gagctttttg gcttcgaaaa tatggggcgc ctgggccaac cctactccgc
    10081 tcatccagcc accgctgatg aaatctgcct tgtcggtgaa gagaagcttc ttgatggccg
    10141 tgacgccctt ctccgcaatt ccgtcatcat cggcaaaaga caactcaact ttattgccta
    10201 agatcccccc cgcggcatta atctcttcgg cagccatctc cgcagcatac tttaccgaga
    10261 ttcctgcttc cttttgcagg gcggccgtga cccccatttt gattaccttt ccctgggcat
    10321 aaatttgggc cgataccccc aaaaccagga aacccacagc gcaaaagata aagaccttgc
    10381 gacccataac cctcctcctt tcatttttgg tatgattttc tttatatccg gtgccttaat
    10441 tttctctgat cacccccttt cttctgggct cctgcaaaaa ataaccccct cagaccttca
    10501 agcccgccaa gaaaccttca tgaatggcct ctttggcgct tcttggctcc acgcaatccc
    10561 cgatgggaag aatctgaggg attttccctc ccagggctcg aagcaggtct tgttcggatc
    10621 gagagccctg ggctgaaatg atggtctccg cctccagcca ctgccttttt cctccctggg
    10681 tgacaagaac acgcccttga ccgagttcca gtacttgggc tgcaaacagg aaggaaattt
    10741 ttgaggccgg cagacgttct aatagaagag cccgggtggt cccttccatg tcggtggcca
    10801 attgctctgg cccctcccgg gaggtaatca cggtaacctg gcagcctctc tcggataaga
    10861 actcagcggt ttcgatcccc accattcctc cgcctgaaat caacacccgc tttcctacct
    10921 ctttcccggc cagcacttcc caggcagtga ggatgctcga actcgcccgg ggaagacgaa
    10981 ctgcggggct ggcccccgtg gccacaatga ccgcgtccgg tttctcctta tcgatgactg
    11041 ccaggtttcc ttcggagcta acccggacat ccaccccttc tctttggatt tccccgatga
    11101 gccaatccat ggatttgaag taattctgct tccgcgggag agccgcggct aagatcatct
    11161 ggccccccag acgcgggctc ttttcgaaaa gggttacccg atgacctcgc ttcttggcca
    11221 cccgggccgc ttccatacct ccaggtcctc cgccgaacac gaaaacatct ttgacctttt
    11281 tcgccgggtg aatctggcat tccttttccc ggcccaccgc agcattctgg aagcaggtaa
    11341 tgtctttctg ctgaaagagc tcccagatac aatggttgca atagacgcaa gggcggatcc
    11401 ggtcctcttc cccgcgggcg gccttggccg gccaatgggg atcactcaga aaaggcctgc
    11461 cgaccgcaac caaatccgct tgtccctctt tcaacacctg ttccatgaac tctggagaat
    11521 ccaaacgccc cacggccacg accgggagcc gcaccatttt cttgatagcc gccgccagag
    11581 ggacatggta gccccgggga aaaaacatgg ggggcatagc gtgcagggaa ctccgcaggg
    11641 tttcattggt tcctcccgag acgtcgatga ccgacacccc cgctcgctcc cattccgcga
    11701 cccaatccaa gacctcctcg agttcatacc cgcccgggat gtattcttgg gcgctgagtc
    11761 gaaagaggac ggggtatgtt ctccccacct gcttccgcac ctcccggaca atctccaagg
    11821 aaaatcgcag ccggttttcc gaacttcctc cgtaggcatc ttttctttgg ttggtcgtcg
    11881 gggacaggaa ttgatggatt aagtatccgt gggccccgtg gacctccacg gcatcgaacc
    11941 ctgcctcctt ggctcgtcgg gcggcggcgc cgaaatcaac gacggtctcc tgaatctctt
    12001 cccgggtgat ctcccggggg atagggtatc caggtccccg ggggatcgga gaaggggcca
    12061 ggggagggcc tccgatgatc tccggactgg cgatccttcc cgcatggtga agctgcaggg
    12121 ccaaggtgga cccgcagtca tgaaccacac gggccaggcg ctgaaggctg gggaggaaag
    12181 catcctcatg ggcgcagatg caccttaacc gatgcctccc cttggaactg accgggcagg
    12241 cttcggagat gatcaagccc gctcctccct cagctctctc cttcaaaaag gcggcaatcc
    12301 gatcgttggc cgttccatcc tcattggaga aattattatt cagggcggcc atgacaattc
    12361 ggttttttag cttcaacccg ccctggataa aggaagagaa aagagtttca taccgggcca
    12421 tcaaccttca tctcctgtgg ccattttggg ccgacgatct tctggaagta aaagacctcc
    12481 tttttcccca cgccttcatt atcatatctc cagaagagag ggaagccctt ttaagggcca
    12541 gaaatttttc caaggaacct ggtcttgtta aaatcactgc cgggccaggg ctatgctgat
    12601 ttcggccaca acttgagcgg ggtcgataaa aggaagaggc aggtccccat cttgcaagac
    12661 gagggggatc tccgtgcatc ccaataatat cgcctgcgct cctgcttcag cgagttcttg
    12721 ggccgcccgc aagagctctg ctctcacctg cgcgcccagg tttccggcct ttatttcgta
    12781 aatgcaattc atgatcttgt cttgggtctc aagccttggg ttcaggattt ttttccggac
    12841 cttcgcaaac gccttggaat agagacccag gaccacggtg gaactacccg ccaaaagccc
    12901 aaaggtttcc aaacggggga atttccgccg gcagtaacgg gctgtctctt cgatcatatg
    12961 caacacgggg accgctacct tcttggccac ctccccgtgg tagtaatggg cggcgttgca
    13021 ggggatcaag agcaaaccgg cccccgaccg ctccagattc tgggcggcgg cgatgagacc
    13081 tcctacaggg ctttccgcct gctcaaagat cgctttgacc cggtcaggaa tcttggggtt
    13141 gcagtcgatg aggatcctca gatgctcctg gtccgtccgg gccggggtgg ctttcacgat
    13201 cttctggaag agatcgatcg tggcttctgg gcccatgccg ccgatgattc cgatcacctt
    13261 ctcggctatg gccgatctct ctccaccttt tttctttgaa cttcgacccg ccttcttcaa
    13321 aaaattcctc tccctcaaat cttcatcgcc gcggcaaaac cctcatgaat cgcttccctg
    13381 gccctccgag gttcaaggca atcaccggca gcaagaagtt gagaaacctt tccttcaaga
//
LOCUS       DASPRR010000564         5767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2074674, whole genome shotgun sequence.
ACCESSION   DASPRR010000564 DASPRR010000000
VERSION     DASPRR010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074674"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VEL68_20790"
     CDS             <1..276
                     /locus_tag="VEL68_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002797141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ38478.1"
                     /translation="SRTIWATLRTANVALGWVFGGALALLILVLYVPFLRELFHFTFL
                     HPDDLLLCLVAGGFSILWFEGLKLFKKRANSSLPVHPSPKRGDSNTA"
     gene            579..746
                     /locus_tag="VEL68_20795"
     CDS             579..746
                     /locus_tag="VEL68_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38479.1"
                     /translation="MKPNAVAFLKEKVILYALMGLILWFFAIPLVTYCFFDSKPYIPK
                     KVASVLLFFFR"
     gene            840..1865
                     /locus_tag="VEL68_20800"
     CDS             840..1865
                     /locus_tag="VEL68_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXZ38480.1"
                     /translation="MPRKTILFGFVFLFTLSFLPPLASAAYKPEYKLSVVVAQNNPWG
                     GAAQQFADLVRERTGGKINIKCYFAGQLFAGMQTNEFLITRQGVGDFAVGSTINWSPT
                     VKELNLFSLPFFFPDYKALDAVEKGDPGKKLFQIIEQRGVLGLAWGENGYRELTNSKR
                     PVRKPEDLEGLKIRVVGSPIFIDTFRAMGGNPLSINWSEALTALQQGTVDGQENPVSS
                     IIIPYKLWEMNKNITIWHYTIDPLILGVSKLTWDSFDPKDREIVAQAAQEAMAWNKKE
                     ARKGLEGSTEAIDMLKSKGMEVVILSPKDMAAFKAKTQSVYDKWIKEIGADLTSAAEK
                     MVKAVTP"
     gene            2066..2548
                     /locus_tag="VEL68_20805"
     CDS             2066..2548
                     /locus_tag="VEL68_20805"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ38481.1"
                     /translation="MMKKSSWFPSRFEEVLCAILFAVMAVITFINIVSRYLLKYSFAF
                     TEELVVSFFVWLTLLGTSIAFREQSHLGFTFLTDRLPPKFQKLLLWISAGLGATLFIF
                     LIYFSIRQVGEEILLGITSSGIGVPQWWYTIGVPLWSILIVLRVFQGAHRVSRKMEKG
                     "
     gene            2548..3831
                     /locus_tag="VEL68_20810"
     CDS             2548..3831
                     /locus_tag="VEL68_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ38482.1"
                     /translation="MEPGIIFFGLFFLLLFIGVPIAVSVGITALVFLWGYNLGIQVMS
                     TNFFANIAKFPLLAIPFFILAGFLMDRVGLSRRLVNLLHLLVGPIPGGLGIVAVLGCV
                     LFGAISGTGPADTAAIGAVMIPAMVKRGYDIGFSSALVASAATTDVLIPPSVAFVVYG
                     VITETSIGGLFAAGVVPGLIMGAALILPVYLISKKHGWGGDRWGTREEIWQALKEAFW
                     GLMAPIIILGGIYGGIFTPTEAAVVAVFYGFFVGAFIYRNLSLQMLYEVLRDSALSTA
                     VVMIVVAFAGLFAWTGSTMGIMDRAAKALLGLTENPVFLLLLLNGLLLIAGMLLDAIS
                     IYYVFLPILIPVIRHFGWDPIWFGVMMTVNLAIGTVTPPVALNLYVAANLASISMEKI
                     SRAAIPFILALIVSLMFVTYIPSLSLWLPKVFGLR"
     gene            complement(3893..5494)
                     /locus_tag="VEL68_20815"
     CDS             complement(3893..5494)
                     /locus_tag="VEL68_20815"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HXZ38483.1"
                     /translation="MPVIFSLMLHSHIPYCRKSGVWPAGEEWLFEAMNETYIPLLTVL
                     RQFKFDDIRPRLMIGVVPVLAEQLADEYMKNRFGQYMEDKIERAGRDIKRFHGDEARR
                     LVAEYWLNRFQRHYHAYRNDFYQDILGTLKWLQDEGVIEVLTSAATHGFLPLMQRDSS
                     VFAQVRVGIDTYRKYFGRSPRGFWLPECAYRPAQWSQEEKRTRKGIDEWLATEGIEYF
                     FVENVGLRRSEFVENLHSESSPTTDCGYHLPSGVAVFGRNDATGRQVWSPEAGYPGDP
                     NYLEFHRKDADSGLHYWRVTGRAEKEVYNPDWALERVENHAVHFVSLLNREGARTQDQ
                     SHKTPPIIVSPYDCELFGHWWHEGPLWIDRLFRKLAHEQGIQCLSLGDFIDRYKTGFS
                     SIKMGPSSWGLNADFTVWQNPDHGWIWPYINTCSREMEQLLLLLEIQGKPTDERGRRI
                     VRQSALELLLLQGSDWPFLLFTTQAKEYANQRFHHHHQRFQKLLWAAKDLSDRNRLKD
                     QELQDMEEVDSLWPDIDPFLFRPRN"
     gene            complement(5534..>5767)
                     /locus_tag="VEL68_20820"
     CDS             complement(5534..>5767)
                     /locus_tag="VEL68_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38484.1"
                     /translation="HWAFLRRLIGGEILERYGSVLPGSGRSAAEELAKSLALPLEMTP
                     GEVEKHLDALVRGGNIRPKHYQNRKTQWEWAEN"
BASE COUNT         1258 a         1615 c         1450 g         1444 t
ORIGIN      
        1 tcgaggacca tctgggctac cctgcgaacc gccaacgtcg ccttgggatg ggtcttcggg
       61 ggagccctag ctctcctcat tctggttcta tacgttccct ttcttcgcga gctcttccat
      121 ttcaccttcc ttcaccccga tgatctcctc ctctgccttg ttgccggagg attcagcatc
      181 ctctggtttg aagggctcaa actcttcaag aaacgcgcca attcgagcct ccccgttcat
      241 ccatccccca aaaggggtga ttccaataca gcatagaaac tgcggggcat acctaatcgg
      301 cggccgatct tttctgctcc tttccctttt gaaattttcc tttgttttgg gaaaaaacct
      361 gagattcgct taattccccc cactttggga aagcggaagg caggcaaaca ccggaaaggc
      421 ccctctccgg ccgtcgatcc ccaaaacccc cgggtgacaa gagtaaaatt tttccttgat
      481 aaaaaaagtt aaaaaggtta ttatcgcacc ctgactcgcc acaggtattt cggtttgttc
      541 cccggcccga aaccttgccc aaggattaag ggaagaaaat gaagcctaat gcggttgctt
      601 tcctgaaaga aaaagttatc ctctacgctc tgatgggcct tattctctgg ttcttcgcga
      661 tccccctggt aacctactgt ttcttcgaca gtaagccata catccccaaa aaggtcgctt
      721 ctgttctgct cttcttcttc cggtaagtca tggaaactga aaggggattc agccagcttc
      781 cgattcgggg agaagatact tcattttagg gaagccaact tttctcggag gaggtaaaaa
      841 tgcccagaaa gacaatcctt ttcggattcg tattcctttt cactctctct ttcctcccgc
      901 ccctggcctc cgctgcctac aagccggaat ataagcttag cgtcgtagtc gcccagaaca
      961 atccctgggg cggtgcggcc caacaattcg cggaccttgt gagggagcgg accgggggga
     1021 agatcaacat caagtgttat tttgcagggc aactcttcgc cggcatgcag accaacgaat
     1081 tcctcatcac ccgccaggga gtcggcgatt ttgccgtggg ttccaccatc aactggtccc
     1141 ctacagtgaa ggagcttaac cttttctccc tgcccttctt tttcccagac tataaggcac
     1201 tcgacgctgt ggagaagggt gacccgggga aaaaactctt ccagatcatc gagcagcgag
     1261 gagtattagg gctggcctgg ggagagaatg gctaccggga gctgaccaat tcaaagcgcc
     1321 ccgtgcggaa acccgaggac ctggaagggc tgaaaattcg ggtcgtgggc tcgcccatct
     1381 tcatcgatac cttccgggcc atggggggaa atcccctttc cattaactgg tccgaagccc
     1441 tgactgcttt gcagcagggg acggtggacg gccaggaaaa cccggtctct tccatcatca
     1501 ttccctacaa actctgggag atgaacaaga acatcaccat ctggcattac accatcgacc
     1561 ccctgatcct gggcgtcagc aaactgacct gggattcctt cgaccccaaa gacagggaaa
     1621 tcgtcgccca ggcggcccag gaggccatgg cttggaacaa gaaagaggcc cgcaaaggtt
     1681 tagagggaag caccgaggcc atcgatatgc tgaagagcaa agggatggaa gtggtgattc
     1741 tttctcccaa ggatatggcc gctttcaagg ccaagaccca gtcggtttac gacaagtgga
     1801 tcaaagagat cggggcggac ctaacttcgg ccgcggaaaa gatggttaaa gcggtcaccc
     1861 cttgaggaga ttcaaaacgg agcgaacctt gaaacgcccc cccgggggcg tttctgcgtg
     1921 agaatccaaa agaaaagcgg tggcatctgg agcctgcccg aaggaaaaat ttctcccctg
     1981 ccgtggcggg agggttgaag gagaggggag aaagattcat gcctttgact agattggctg
     2041 atatccaccc cggacacact gaaggatgat gaaaaaatcc agttggtttc cttcccgttt
     2101 tgaagaggtt ctttgtgcga ttcttttcgc ggtgatggct gtcatcacct ttatcaacat
     2161 cgtctcgcgt tacctcctga aatattcttt tgcctttacg gaggagcttg tcgtcagttt
     2221 cttcgtctgg ctgactcttc ttggcacatc catcgccttc cgggaacaat ctcacctggg
     2281 attcaccttt ctgactgatc gtttaccgcc aaagtttcag aaactccttc tctggatcag
     2341 cgcgggattg ggcgcgactc ttttcatctt tttaatctat ttttccatcc gtcaggttgg
     2401 tgaggagatc ctcctgggaa tcacctccag cggaatcggc gttccccagt ggtggtacac
     2461 gatcggagtg ccgctatgga gcattctgat cgtcctgcgt gtttttcagg gagcccatcg
     2521 ggtcagccgg aagatggaga aggggtaatg gaacccggga tcatattttt tggcctgttc
     2581 ttcctcctcc tttttatcgg ggttcccatc gccgtcagcg tggggatcac tgccctggta
     2641 tttctctggg gatacaacct gggaatccag gtcatgtcca ccaatttttt tgccaatatc
     2701 gccaagtttc ccctgctggc cattcccttt ttcattctgg cgggattcct catggaccgc
     2761 gtgggccttt cccgtcgcct ggtcaatctc ctgcatctcc tcgtcgggcc gattcccgga
     2821 ggattgggaa ttgtcgccgt gctggggtgc gtccttttcg gggccatttc gggcacggga
     2881 cctgccgata cggcggccat cggggcggtg atgattccgg ccatggtgaa gaggggatac
     2941 gatatcggct tctcctcggc cttggtcgcg tcggcagcca ccaccgatgt cttgatccct
     3001 cccagcgtgg cctttgtcgt ctacggggtc attaccgaaa cttccatcgg aggtctattt
     3061 gcagctgggg tcgtacccgg tctgatcatg ggagcggccc tgattctccc cgtctacctg
     3121 atctccaaaa aacatggctg gggaggagac cgctggggca ctcgagaaga aatctggcag
     3181 gcattgaaag aggccttctg gggtcttatg gcccccatca ttattctcgg gggaatctac
     3241 ggaggtattt ttacccccac ggaagccgcg gtggtagccg ttttctacgg cttttttgtg
     3301 ggggcgttca tctatcggaa cctgtctctt cagatgcttt atgaagtctt gcgggattcg
     3361 gcattgtcca cggctgtggt aatgatcgtg gtggcctttg cgggtctgtt cgcctggact
     3421 ggttctacca tgggaatcat ggaccgggcg gccaaggcgc tcttgggcct aaccgagaac
     3481 ccggtctttc ttctcttatt gctaaacggt ctcctactga tcgccgggat gctgctggac
     3541 gccatttcca tctactacgt ctttcttccc atcctgatcc ctgtcatccg gcatttcggc
     3601 tgggatccca tctggttcgg cgtgatgatg accgtcaacc tggccatcgg caccgtcacc
     3661 cctccggtcg ccttgaacct ctatgttgcg gccaacctgg ccagcatctc catggagaag
     3721 atatcccggg ccgcaattcc cttcattctg gcactcatcg tctccctgat gtttgtaacc
     3781 tatatcccct ccctctccct ctggctcccc aaagtgtttg gccttcgcta aaatcgagtc
     3841 ttgttttttg ggccacggtt ttaggggtta agaatatctt atttcgtttg tattaattgc
     3901 gcggacggaa gagaaagggg tcaatatccg gccaaagaga atcgacttct tccatatctt
     3961 ggagctcttg atccttcagg cgattccggt cagaaaggtc tttggctgcc cagagaagtt
     4021 tttgaaaccg ctggtggtga tgatgaaacc gctgattcgc gtactccttg gcctgggtcg
     4081 taaacaagag aaagggccaa tcactccctt gcagcaaaag gagttcgagg gccgattgac
     4141 gaacaatgcg gcgcccccgc tcatcagtag gtttaccctg aatttcgagg agaaggagta
     4201 attgttccat ttctctcgag caggtgttga tgtaaggcca aatccagccg tggtcagggt
     4261 tctgccaaac ggtgaaatcc gcgttcaatc cccatgagct tggacccatt ttaatggacg
     4321 agaagccagt tttgtaacgg tcgataaagt cacctaaact caaacactga atgccttgct
     4381 catgggccaa tttgcgaaag agacggtcaa tccataaggg accctcgtgc caccaatgtc
     4441 cgaacaactc gcaatcgtaa gggctcacaa taatgggagg agtcttgtgg ctctgatcct
     4501 gagtccgagc cccttctcgg ttgagcaagg aaacaaaatg gaccgcatga ttctcgactc
     4561 gttcaagcgc ccaatccggg ttataaacct ccttttcggc ccgcccggtc acccgccagt
     4621 aatgcagccc tgagtctgca tcttttctgt ggaattccag gtaattcggg tctccgggat
     4681 aacccgcttc aggggaccag acctgccttc cggtggcatc attccttcca aataccgcca
     4741 cccccgaggg cagatgataa ccgcaatccg tggtcgggga agattcggaa tggagattct
     4801 cgacaaattc actgcgtctc aggccgacat tttccacaaa aaagtactca atcccctcag
     4861 ttgccaacca ttcatcaatc cctttcctgg tgcgcttttc ctcctgggac cactgagccg
     4921 gacggtaggc gcactcagga agccagaacc cccgcgggct gcgcccaaag tatttccgat
     4981 aagtgtcgat accgacgcgc acttgggcga acacggaaga atctcgctgc ataagcggga
     5041 gaaaaccatg ggttgccgca gaggttaata cctcaatgac cccctcatcc tgtaaccact
     5101 tcagagtgcc caggatgtcc tggtaaaaat cattccggta agcatgatag tggcgttgga
     5161 atcgattcag ccaatattcg gccacaaggc ggcgggcctc atccccgtgg aatctcttga
     5221 tgtctcgccc agcccgctcg atcttatctt ccatatattg accaaagcga tttttcatgt
     5281 attcatccgc cagttgttcc gcgaggacag ggacgacacc aatcatcagg cgcggccgga
     5341 tatcatcgaa tttgaattgc cttagcaccg taagcaaggg aatataggtc tcgttcatgg
     5401 cctcaaacag ccactcttcc cccgccggcc acaccccgga tttccgacag taggggatat
     5461 ggctatgcaa cattaacgaa aaaataactg gcatctattt accccttttt tcttgcatga
     5521 acctatttaa aattcaattc tcagcccact cccattgggt ctttctattt tgatagtgct
     5581 tcgggcggat atttcctccg cgaaccaggg catctaaatg tttttcaacc tctcctgggg
     5641 tcatttccag aggaagggct agagatttgg ccagttcctc cgcagcactc ctgcccgatc
     5701 ccggaaggac actcccataa cgctccagaa tctctccccc gatcaaccgc cgtaaaaagg
     5761 cccagtg
//
LOCUS       DASPRR010000565         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1908603, whole genome shotgun sequence.
ACCESSION   DASPRR010000565 DASPRR010000000
VERSION     DASPRR010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908603"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..679
                     /locus_tag="VEL68_20825"
     CDS             251..679
                     /locus_tag="VEL68_20825"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ38485.1"
                     /translation="MSGYVLLTIGVITAWSSTSLSMGKASRPGPGFLPFGLAIILIAL
                     SLALILGSWKKESDSVPFWPKRGWLRPLVGVGIFLLYALILGPLGFIFTTFLFLILWM
                     WIIERIRWVTILTISVGVTGVLYFIFEYFLEVPLPAGFLG"
     gene            694..2205
                     /locus_tag="VEL68_20830"
     CDS             694..2205
                     /locus_tag="VEL68_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ38486.1"
                     /translation="MDEILGMFQGLYSGFGIAFSLINLTFALIGAVLGTVVGILPGLG
                     PAATISLLLPVSFKIGSPVTSIIMMAGIFYGAMYGGSTTSILVNIPGEAASVVTCIDG
                     YQMALKGRAGPALGIAAIGSFVAGTIGVLGLTFVAPPLAELALKFGPPEYFSLTLFGL
                     LMATLLGEGSILKGLIMVVMGLLLGSVGLDPISGAIRFTWGLHMLQEGIDFVTLAMGM
                     FGMGEIFYNLEKELKTELVTRKVAHLWPTLKDWADSKWAVIRGSFIGFFIGILPGGGA
                     VISSLVSYAVEKKVSKTPEEFGKGAIQGVAGPESANNSAASASFIPLLTLGIPGNAAI
                     AMIFAALLIQGVQPGPFLITERPDIFWGVVASMYIGNVMLLILNLPMVGLWVQLLRVP
                     YAILAPIIVLFCCVGVYSIRNTVFDIYVMGIFGVIGYILRKVNLEPGPMILAFVLGPI
                     MERAIRQALLISAGSPLIFFTRPISGTLMGLLILFIVLQVFIVLRKKKTSNHT"
     gene            2238..>2472
                     /locus_tag="VEL68_20835"
     CDS             2238..>2472
                     /locus_tag="VEL68_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ38487.1"
                     /translation="MKRLFYGMVLGVFLLCPVLGMAAEKFPAKAIELDVPFAAGGSTD
                     VLARLVSKFAPKYFDKPLIVVNKPGGGGITGTEG"
BASE COUNT          478 a          697 c          661 g          636 t
ORIGIN      
        1 gcttggagga atctcctctc aggcctctcc ctgcggaaca tggggaggag tttcatggaa
       61 ggataagcac ccgccgagtc tgatgatcca aattcctcca ccctgaaccc caacaagcac
      121 gaaaaactgg gggtgggcaa gaacaatgat tccccttgac aacttgcagg gctgaagggt
      181 actttttatc cattgtcgaa aaattaattt tttgacttaa aggtgtggag ccatcgaaaa
      241 gtgggatcga atatcggggt atgtccttct gaccatcggg gtgatcacgg cctggagttc
      301 cacttccctg tccatgggca aggccagccg acccggacca gggttccttc ccttcgggct
      361 ggccattatc ctgattgctc tttctttggc cctcatcctg ggaagctgga aaaaagagag
      421 cgattctgtc cccttctggc ccaaacgcgg gtggctccgt cctctggtgg gagtgggaat
      481 attccttctc tatgccctta tcctggggcc gctgggcttc atttttacca cttttctttt
      541 tctcatcctc tggatgtgga tcatcgaacg gatccgctgg gtaaccatcc tgacgatctc
      601 cgtaggggta accggagttc tctacttcat ctttgaatat tttcttgaag tgcctctccc
      661 tgcgggattt ctgggctgac caagggattt aacatggatg aaattttggg aatgttccaa
      721 gggctgtaca gcgggttcgg cattgccttc agcttaatca acctcacttt tgccctcatc
      781 ggggcagtac tgggcaccgt tgtagggatt ctgcccggcc tggggccggc agccacgatc
      841 tctcttctcc ttcccgttag ctttaaaatc ggctctcccg tcacttccat tatcatgatg
      901 gccggaatct tttatggggc catgtacggc gggtcgacca cctccattct ggttaatatc
      961 cctggggagg cagcctcggt cgtaacttgc atcgacggat atcaaatggc gctgaaaggc
     1021 cgggcaggtc ctgccctggg catcgcggct atcggctcct ttgtcgccgg gaccatcggg
     1081 gttctcggcc ttaccttcgt ggctcctccc ctggcagagt tagccctgaa gttcggcccg
     1141 ccggaatact tttccctgac cctcttcggg ctcctcatgg ccacccttct gggagaaggc
     1201 tccattctga aggggctgat catggtggtc atgggcctcc tccttggctc cgtgggtttg
     1261 gaccccatct ccggcgctat ccggttcact tggggtctcc acatgctcca ggaggggatc
     1321 gatttcgtca ccctggccat gggcatgttc ggcatggggg agattttcta caacctggaa
     1381 aaggaattga agaccgagct ggtgacccgc aaggtcgctc atctctggcc tacactcaag
     1441 gactgggccg attccaagtg ggccgtgatc cgcgggtctt tcatcggctt tttcataggg
     1501 attcttccag gtgggggcgc agtgatttct tccctagtct cctacgcggt ggagaaaaag
     1561 gtttccaaaa ccccagaaga attcggaaag ggcgccatcc agggggtagc gggcccggag
     1621 tcggccaaca actctgcggc cagcgcctcc tttattcccc tcctgactct gggaatcccg
     1681 ggaaacgcgg ccatcgccat gatctttgcc gctctcctca tccagggggt tcagcccggg
     1741 ccgttcctga tcaccgagcg gccggatatt ttctggggag tcgtagcttc catgtacatc
     1801 gggaacgtaa tgctcctgat cctcaacctc cccatggtcg ggctttgggt tcagctcctc
     1861 cgagtccctt acgccatcct ggcgccgatt atcgtcctct tctgctgcgt gggagtctac
     1921 agcattcgga ataccgtctt tgacatctat gtgatgggga tcttcggggt catcgggtat
     1981 atcctccgaa aagtgaacct cgagcccggc cccatgatcc tggcctttgt cctaggcccg
     2041 atcatggaga gggccatccg ccaggcgctc ctcatctcag cgggaagccc gctcattttc
     2101 ttcacgcgtc ccatttccgg gaccttaatg gggcttttga ttctttttat cgtgcttcaa
     2161 gtatttatcg tcttgcgaaa gaaaaagact agcaaccaca cctgaaaaaa aatctctcac
     2221 catggaggaa accaaagatg aaaagattat tctatggaat ggttctcggt gttttcctgc
     2281 tctgcccggt ccttgggatg gcggcggaaa aatttcctgc caaagctatc gagttggatg
     2341 tcccttttgc agccggaggg tccacagatg tcttagcccg tctggtatcg aaattcgctc
     2401 ccaaatactt cgataaaccc ctgatcgtcg taaacaaacc cggtggcgga gggatcacgg
     2461 gaacggaagg gg
//
LOCUS       DASPRR010000566         6027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1104962, whole genome shotgun sequence.
ACCESSION   DASPRR010000566 DASPRR010000000
VERSION     DASPRR010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104962"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VEL68_20840"
     CDS             complement(<1..513)
                     /locus_tag="VEL68_20840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ38488.1"
                     /translation="MLKLEGVETWYGAIQAIKGISLDVIKGEIVALLGANGAGKSTTI
                     KTITGILKATKGRIEFLGKDITGMEAEDIAEIGIACVPEGRRIFPGLTVTDNLMLGAT
                     PRKRASKEEIQRDLARVFQIFPQLEKFKGSLGWQLSGGQLQMLAIGRGLMASSKILLL
                     DEPSLGLAPVV"
     gene            complement(506..2338)
                     /locus_tag="VEL68_20845"
     CDS             complement(506..2338)
                     /locus_tag="VEL68_20845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXZ38489.1"
                     /translation="MNTRTKSLLVPAALAVVVLIFPRLVTSNYWMNLINLAISFSIAC
                     LGLNIVLGYAGQLSLAQAAFWGVGAYTSAILTTQLGLPVWAGMFAAFFVAAFFGILLG
                     IPTLKLSGHYLAMATIGFGVILQLILVNAIWLTNGSDGIPKIPSPWIGSLELKDPGTI
                     FYVAAVALVLLTWGAIHLKDSRMGRAFMAIRENEMAAGTAGVDTTYYKIMAFSLSAGY
                     AGFGGWLFAHSGSHYISPDTFSFEQSVIMLVMAVLGGNGSAIGSIVGAILLTLLPEVL
                     RFLKDSYMMVYAAGIILIMIFMPSGIAGLVKNLPVSARLREWWRDGSTAARRVAAATS
                     TLKSPDAALPATFSNCSGSNEVLLTVKGLAKHFGALRAVDGVDMEVRRGMIHAVIGPN
                     GSGKTTILNMLSGLYVPTAGEILLEGKAISGQKSHHITSQGMARTFQNIRLFGELSVL
                     ENVLIGQHYHTRSGLLTSVLQPPSQKAEEASMRKKALEMLAFVGLKGKEFAQANSLPY
                     GQQRLLELARALASDPKLLLLDEPAAGLNAAETDALVELLFQICDRGITILLVEHDMS
                     LVMNVSDHITVLNFGKKIAEGSAEEVEHNQEVIDAYLGTEVSNA"
     gene            complement(2350..3231)
                     /locus_tag="VEL68_20850"
     CDS             complement(2350..3231)
                     /locus_tag="VEL68_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38490.1"
                     /translation="MDWSTNLQMVAAGLAMGSIYALVALGFVLIFNAVNVVNFAQGEF
                     AMVPAFVAVWLLSSLKIPLALTCVITVVFMGAFGIVFQRIAYYPLRNRSFLPVVISTI
                     GVGIFLKNGAQMIFGAEPLSMPRPTSATAVNMLGVLVDPQYIVIIICTLALLGFQYFF
                     FEKTLLGKKMLATAQDKQTARLLGIRVTTMIAITFAYSSILGAAAGILVGPIFFVTKD
                     MGAMIGLKAFCSTIVGGFGSVPGAILGGIFLGVIEVFAAYYVSTAYRDAFAFIILILV
                     LLLRPQGFFGEKIAEKA"
     gene            complement(3326..4465)
                     /locus_tag="VEL68_20855"
     CDS             complement(3326..4465)
                     /locus_tag="VEL68_20855"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38491.1"
                     /translation="MSHFKVFGVALLSISLILSFSSIAMAADEIRIGFTPPITGQHAA
                     SGSLQMKAIKLALKEINAAGGVNGKKIDLRVVDNQSTNPGALASLQKAVDQEKVLALM
                     GFLYSTQVFATSDAIKNYGVPTFIGATNVSLTRQGNPWIFRVRPDDSIAAGAMVKYIK
                     EDTKFTKIGLLHDSDAFGTGGADLVEKSAKEAGLTVVKREKSQTAAKDFTAELLAMKN
                     AGAEILIFYGHQEATAVIQRQMRQLGSPFKFLGSPSAASRDALNLAKEAAEGIWAVVD
                     FVPGVTEENKRFAAAYKKEYNNEEYDNVSVWAYDGLNILVNAIKKAGEDRDKIRQAIL
                     ATQGYKGVQGNFSFTPNGDGLSEVSIVQIEKGQQKLLKIINLGKK"
     gene            4934..>6027
                     /locus_tag="VEL68_20860"
     CDS             4934..>6027
                     /locus_tag="VEL68_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38492.1"
                     /translation="MRGSEMNHRERMRLAIRGELPDQLAYVPRIDLWYNANSRAGTLP
                     EKHKGRTQDEISRAEGWALHKVLADLLDQRGPEDMLHRDLGIYATRQAAFRYRFSHAV
                     DIRVTREGFETRIEYHTPVGTVRTRQVYTEDLKKAGSTIPFLAEHAIKEKGDYRVLAY
                     LFENLELIPDYGDWEKWAAGVGEDGVCAACITPAASPMHHIQREFLDPTDFYFHYHDN
                     YKEMTLLAESMKAHFEQLLKIAVECPAELIFWGGNFDDMITYPPFFEKEILPWMQRVS
                     HVLHDRGKLVHSHCDGENQGLLDLIRNSGIDMAEAICPYPMTKVPIEEYYRRWGDRLT
                     LFGGIPSNMLLADMTPEEEFERYLDHLFKA"
BASE COUNT         1344 a         1763 c         1677 g         1243 t
ORIGIN      
        1 cacgaccggc gccagcccca gagagggctc gtccaaaagc agaatcttcg agctggccat
       61 cagaccgcgc ccgatggcca gcatctgaag ctggccgccg gacaactgcc agcccagact
      121 tcccttgaat ttctcgagct ggggaaagat ttggaagacg cgggcaaggt ccctttgaat
      181 ctcctccttg gacgcccgct tcctcggcgt cgcgcccagc atcaggttgt ccgtcacggt
      241 gagccccgga aagatgcgcc gcccctccgg cacacaggcg atcccgatct ccgcgatgtc
      301 ttcagcttcc atccccgtga tgtccttccc caggaactca atccggccct tagttgcctt
      361 caagatgccg gtgatggtct tgatggtcgt gctcttgccg gcaccatttg cgccgagcag
      421 ggccacgatc tcgcctttaa taacgtccaa cgaaatgccc ttgattgcct gaatggcccc
      481 ataccaggtc tcgactcctt cgagcttaag cattgctcac ctccgtgccc agataagcgt
      541 caatcacttc ctggttatgt tcgacttcct cggcggagcc ctcggcgatc ttcttgccga
      601 agttcaagac cgtgatatgg tcggacacat tcatcaccag gctcatgtcg tgttcgacca
      661 gcaaaatggt gatgccccgg tcgcagatct gaaagaggag ttccacgagc gcatcggtct
      721 cagcagcgtt caatccagcc gcaggctcgt ccagcaggag cagtttcggg tccgaggcca
      781 gggcccgcgc cagctcaagc agccgctgct gtccataggg caggctgttg gcctgggcaa
      841 actccttccc cttcaggccc acgaaggcca acatctccag cgccttcttg cgcatgctcg
      901 cttcttcggc cttctgggag ggcggctgca gaacgctggt gagcagaccc gaccgggtgt
      961 gataatgctg gccgataagg acgttttcca aaacgctgag ctcgccgaaa aggcggatgt
     1021 tctggaacgt ccgggccatg ccctgtgagg tgatgtgatg cgacttctgc ccgctgatgg
     1081 ccttcccctc caggaggatt tctccggccg tcgggacata caggccactg agcatattga
     1141 ggatcgtcgt cttcccggaa ccgttcggcc cgatcacggc atggatcatc ccccttcgga
     1201 cctccatgtc cactccgtct actgccctga gagccccgaa gtgcttggcc agccctttga
     1261 ccgtgagcag cacctcgttc gatccagaac agttagaaaa tgtggccgga agcgcggcat
     1321 ccgggctctt cagggtggag gtggctgccg caacccgccg ggccgccgtc gaaccgtccc
     1381 tccaccactc ccgcagccgc gcactgacgg gcaggttctt caccaggccg gcaatcccgc
     1441 tcggcatgaa gatcatgatg agtatgatcc ccgcggcata aaccatcata taggaatcct
     1501 ttagaaaccg cagaacctcc ggcaggagcg taagcaggat ggcccccacg atggagccga
     1561 tcgccgagcc atttcccccc aatacggcca tgaccagcat gatcaccgac tgctcaaagg
     1621 aaaaggtgtc cggactgatg taatgcgaac cactatgggc gaacagccag cccccgaatc
     1681 cggcgtagcc cgccgaaagc gaaaaggcca taatcttgta gtaggtagta tccaccccgg
     1741 ctgtcccggc cgccatctcg ttctcccgga tggccatgaa ggcgcggccc atacgggaat
     1801 ctttgagatg gatggcgccc caggtcagaa gaaccaaagc aaccgccgcc acatagaaaa
     1861 tggtgccggg gtctttcaac tccaaggacc cgatccaggg cgaaggaatc ttggggatgc
     1921 cgtcggaacc attggtcagc cagatcgcat tgactaggat gagctggagg atgaccccga
     1981 acccgatcgt cgccatggcc aggtagtggc cagacagttt gagggtggga atccccagca
     2041 ggatcccgaa gaaggccgcc acgaagaagg cggcgaacat gcccgcccat acgggcaaac
     2101 ccaactgggt ggtgagaatg gccgaggtgt aggcgcccac tccccagaac gccgcctggg
     2161 ccagcgacag ttgacccgcg tatcccagta cgatgttcaa tcccaggcag gcaatcgaaa
     2221 agctgatggc caggttgatc aggttcatcc agtagttact cgttaccaga cgggggaaaa
     2281 tcaaaacgac cacggccaga gcggcaggaa cgagcaggct ttttgtgcgt gtgttcatcg
     2341 gttcaccctc tatgccttct cggcgatctt ctcgccgaaa aacccttgcg gacgcaaaag
     2401 cagaaccagg atcaggatga tgaaggcgaa ggcgtcacga taggcagtgg acacgtaata
     2461 ggccgcgaat acctcgatca cgcccagaaa aatccccccc aggatcgccc cggggacact
     2521 cccgaatcct cccacaatgg tcgagcagaa tgccttgagc ccgatcatcg cgcccatatc
     2581 cttggtcacg aaaaaaatgg gcccgaccag gattccggcc gccgccccca gaatcgaact
     2641 gtaggcaaag gtaatggcga tcatcgtcgt cacccggatg cccaggagcc gcgccgtctg
     2701 tttgtcctgc gccgtggcca gcatcttttt gcccagcaga gtcttttcga aaaagaagta
     2761 ctggaagccg agcagggcca gggtgcagat gatgatcaca atgtactggg ggtccacgag
     2821 cactcccagc atgtttactg cggtcgcgga ggttggccgc ggcatcgaca acggctccgc
     2881 accaaagatc atctgagccc cgttcttcag aaagatgccc acgccgatgg tgctgatcac
     2941 gaccggcagg aagctacggt tccgcagcgg atagtaggcg atacgctgga agacaatgcc
     3001 gaaggcaccc atgaagacga cggtgatcac gcaggtcagg gcgaggggga ttttcaggct
     3061 gctcaacagc cacacggcta caaaggccgg gaccatcgca aactccccct gcgcaaagtt
     3121 gacgacattg actgcattga aaatcaggac aaatcccagg gcaaccaagg catagatcga
     3181 acccatggcc agacctgcgg ctaccatttg aagatttgtg gaccaatcca ttcaatgcct
     3241 ccttaaaaag acacgtccag tcccggcatg tcgccatgcc gctggactgg acgtctctct
     3301 tctcgatacg ggcgccggga ggtgattatt tcttacccag gttgatgatc ttcaggagtt
     3361 tctgctggcc tttctcgatc tggacaatgc ttacctcgct caggccgtcg ccgttcgggg
     3421 taaaggaaaa attgccttga acacccttat atccctgggt ggccaggatg gcttgacgga
     3481 ttttgtcgcg atcttctcca gccttcttga tggcgttgac caggatgttc agaccgtcat
     3541 aggcccaaac gctgacattg tcgtactcct cgttgttgta ctctttcttg taagccgcgg
     3601 cgaagcgctt gttctcctcg gtcacgccag gcacgaaatc gaccacggcc caaatcccct
     3661 cggcggcctc cttggcgagg ttcagggcgt ctctcgatgc ggcgctgggc gaaccgagaa
     3721 acttgaaggg agagccgagc tggcgcattt ggcgctggat caccgccgtg gcttcctggt
     3781 gcccgtagaa aatcaggatc tccgctcctg cgttcttcat ggccaggagc tcagcggtaa
     3841 agtccttcgc cgctgtctgg gacttctccc tttttaccac ggtcagaccc gcctccttgg
     3901 cgcttttttc caccaggtcg gcgccgccgg tgccgaaggc gtccgaatcg tgcaagagtc
     3961 cgatcttggt gaacttggtg tcttccttga tgtacttcac catggctccg gcggcgatgg
     4021 agtcatcggg ccgcacgcgg aatatccatg ggtttccctg cctggtaagg ctgacattgg
     4081 tggcgccgat aaaagtgggg acgccgtaat tcttgatggc atccgacgtg gcaaagacct
     4141 gggtgctgta aaggaagccc atcagggcca gcaccttctc ctgatccacc gccttctgca
     4201 gggacgccag cgcgccgggg ttagtggatt ggttgtcgac aacccggaga tcgatcttct
     4261 tgccgttcac cccgccggcg gcattgatct ccttgagggc cagtttaatc gccttcattt
     4321 ggagagatcc cgatgccgcg tgttgaccgg tgatgggggg agtaaatccg atccgaatct
     4381 cgtcggcagc catcgcaatg ctgctgaatg ataaaatcag cgaaatcgac agcaaagcaa
     4441 ctccaaagac cttgaaatgt gacatgcact ttctcctttt gttggttagt gctccccacc
     4501 ctgtggggaa agcaagaaga tattagatta cctcttccaa aaatgtcaag aatatagagt
     4561 aaagaaaatg gtcaggtttc aacccatgac aaatcggggt gatccttgat ccggtacgag
     4621 agaaattctt cagggggtat ttattggccc ggcctctccg aatcaaattc ccttctcttg
     4681 gaaaccccgc gaggcaattt atcccaagtc ctttgccctc taaacggggc ttcggaagac
     4741 gcttctcctt cctcgtctct cggagctatc aattcttttt taaactgaaa taaggtaaaa
     4801 tggcttttct attatcacca tcgacgcgcg ccttctgtag gattcaggag gcaaaacaga
     4861 aaatggggaa gtctatcggg cctcatgagg aatggtaaaa ggctaaagcg tcagcggttc
     4921 caatgagaag ccaatgagag ggagcgaaat gaaccatcga gagagaatgc gcctggcgat
     4981 tcgaggagaa ctcccggatc agcttgccta tgttcccaga atcgacttgt ggtacaacgc
     5041 caattcccgg gcgggaacgc tgccggaaaa acacaaggga cggacccaag acgagatttc
     5101 ccgggccgaa gggtgggctc tgcataaggt tttggcggat ctcctcgatc aacggggacc
     5161 cgaagatatg ctccatcgcg atttggggat ctatgccacc cggcaagcag ccttccgtta
     5221 ccgattttcc cacgccgtgg atatccgggt cacccgggag ggatttgaaa cgcggattga
     5281 ataccatact cccgtgggaa cggtgaggac acggcaggtc tacacggaag acctcaagaa
     5341 ggccggatcg accattccct ttctggccga gcatgccata aaggaaaagg gggactaccg
     5401 ggttctggct tatctctttg aaaatctgga gctgatcccc gattatgggg attgggaaaa
     5461 gtgggccgcc ggggtgggcg aagacggggt ctgtgcggct tgcatcacgc cggcagcttc
     5521 cccgatgcac catatccaac gggagttcct ggaccccacg gacttttact tccattatca
     5581 cgacaactac aaagagatga ccctgctggc cgaatctatg aaagcgcatt ttgagcagct
     5641 tttaaaaatc gctgtcgagt gcccggcgga attgattttc tgggggggca atttcgacga
     5701 catgatcacc tatcccccat tcttcgaaaa agagattctt ccgtggatgc agagggtatc
     5761 tcacgtcctc catgatcggg ggaagctggt gcattcccac tgcgacggag agaatcaggg
     5821 gctcttggac ctgatccgta actccgggat cgacatggcg gaggccatct gtccctatcc
     5881 catgaccaaa gtccccatag aggaatacta ccggaggtgg ggagaccgct tgaccctttt
     5941 cggcggcatc ccctccaata tgttgctggc cgatatgact ccggaggagg aatttgagcg
     6001 gtatctggac catctgttca aggcgat
//
LOCUS       DASPRR010000567         8590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_792623, whole genome shotgun sequence.
ACCESSION   DASPRR010000567 DASPRR010000000
VERSION     DASPRR010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792623"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="VEL68_20865"
     CDS             complement(<1..1815)
                     /locus_tag="VEL68_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38493.1"
                     /translation="MKKSRKYLLIISISLVLILGGLASVGFWALNSAEGTRVLLKTIS
                     LLTPLRIEAREISGRVKDELKMRGLRIRWPQGELQADSFHLRWEPGDLWNRKLLIREL
                     SLEGVCVADNRPETGPISFRGWPVTPFWLSRLQGQVDTLRVERGFYRRLQENPIPFDA
                     LSARLEWDGEVLKVQDFILEGPSVKAEGSMKMGFSTPSFSLDLQAALADEIAGLDSFQ
                     VQLGLEPVRAQEEAKGFFSLSARRKAVQEFHLQGNLGLTRSVLQFQNLRLLQPGTKGK
                     IQGEGEIAFGEKPTFSLKADFSNITLVPELDLLSNLTGTIEIKGPLDTYRGHLTVANL
                     AKGWQKAQASAVFRGNLEYLEVTTLMGRWLDGSVKGPLRISWANGFSLQGSLQGRKLN
                     PALLTPDISGEINLNLDGRLLWPKTGKPGAAFDANLLESRFLDKAVTGALEGRWEGSL
                     LNIAHLRLRGRGFDLRGKGILQERITLEGKVTDLSQFITQAKGQISGAGWFRYQEDRL
                     SGAMVAESKELWTQEARAGDLRAEIQLKEYGPKISPVLSLEARAGNIKAEAVDLSSVR
                     FQADGSPSFHRAQFAASVNRVELQGQVTGAYQDRSWKGS"
     gene            complement(1812..3506)
                     /locus_tag="VEL68_20870"
     CDS             complement(1812..3506)
                     /locus_tag="VEL68_20870"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HXZ38494.1"
                     /translation="MAAEALKVVIEGIQGDALKNAQAALALPPGMVREGVIDRALLEV
                     FRHQISEKVQKALEPFGYYEAQVSSAQEKGEEGQEFLRVKVIPGSPIRVASMIVRISG
                     PGETNPDLKQLVAAFPLKAGDVLNQPKYEKAKEELRTKALNQGYLGSEYTSHVIRVDR
                     GEHKAEIELILQTGSQYLFGEVIWEGTQLYPVSYLERFLDFKPGQPYSYSKIYQTQLN
                     LINSDRFSSVNIRADKEEAHEGRVPVRIHIEPSAPKRLRPGVGYSTDYGAQVSLHYQD
                     VNAFKRGHEFNADATVAQRRQAISSYYSIPGSGHVDNRTNLKVGLQRELLKPYDNLLF
                     TTEVEQARSFGYGTVGSAYLQFREERFSEAGVEGSSNLFMPGLRFSQRRVDDLIRPGK
                     GFRYALETRGSTQALGAETNFLQFLGNADFLIPLGAGFSLIPRSQVGVTWQKDPLTDL
                     PPSLRFYAGGDRSVRGYTYQSLGPKDSSGNVIGGKNLWVGSLELEYAITRKWSVAGFY
                     DVGNAFNNLDNLQLAQGAGLGFRYYTPAGPVRVDLARQINVEHPGFQLHISIGFAL"
     gene            complement(3803..4603)
                     /locus_tag="VEL68_20875"
     CDS             complement(3803..4603)
                     /locus_tag="VEL68_20875"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXZ38495.1"
                     /translation="MSEIERIVNLAHSTLNRFRLDQRAAMVVGGSQGLGQGMALALAA
                     AGADVCVVGLGAKNLEETASAISKLGRKSIWIEADMTLEENVKRTVSQALEGFRRIDI
                     LVNSQGISHLQPSAEFDVEAWQRVMDVNLKSLFLSCKHVGRVMLTQGKGKIINISSVR
                     SFQGRAEDLAYAPSKGAVNQLTRSLAIEWGVKGINVNAIAPVFTLTPMNRSILDDPEK
                     RDWVLGRIPLRRLGELEDLFGPVVFLASEASNFVNGHILIVDGGWIAA"
     gene            complement(4658..5749)
                     /gene="ugpC"
                     /locus_tag="VEL68_20880"
     CDS             complement(4658..5749)
                     /gene="ugpC"
                     /locus_tag="VEL68_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXZ38496.1"
                     /translation="MAKVLMEKLNKHFGEVKAVRDFDLEIPDKEFVVLVGPSGCGKTT
                     TLRMVAGLEDITSGDILIDDKVVNNLPPKDRDIAMVFQNYALYPHMTVYQNMAFGLTL
                     RKFPKEEIDQRVKDAAEILNIKELLDRKPKALSGGQRQRVAVGRAIVRKPKVFLFDEP
                     LSNLDAKLRVQMRVELKKLHERLQTTIIYVTHDQVEAMTMGDRIVVMKDGLKQQVGPP
                     LELYFHPANKFVAGFIGSPAMNFIEGELFSEGGAVHFQGPGMKLKIPGDKASKVKGYS
                     EKRVIFGVRPEDLPEAPCAAPGETFDVTVEVMEPLGSEVYLDVKAAEQSLIARAEPNT
                     TAKPHARLCLQPNMENIHLFDIRDEKTLV"
     gene            complement(5799..6941)
                     /locus_tag="VEL68_20885"
     CDS             complement(5799..6941)
                     /locus_tag="VEL68_20885"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ38497.1"
                     /translation="MNSRERLLVALNHREPDRVPIDLGGTPTSTISARAHENLKAHLG
                     IQSPTRMMSPIFLTAYPDEEIVRRFGVDVKMLTANPPAEFELHSTPEGKIVDEWGVVY
                     QWNEEAQTHFVVEREAPLHRVTTPKEVEKHPWPDPTDPSRYRGLKEIAKRYQEEGYGV
                     MASAPLMVMTQTQGMRGLEQFMMDSVLNPSLLEYMMDKILGIQLEMTRHLLEEIDPHM
                     DVIVIGDDLSHQRGITYSPDKYRQLFKPRHRKIMRVLKEEFGDAKVLYHCCGGVEPLL
                     PDLIDLGIDAINPVQVSAKGMGDTKRLKAKYGRHLTFWGGIDTQWILPFGTPLEVQNE
                     VRRRIEDLAQDGGFVLGAVHNLRPEVKPENICALYAAALEYGKYSK"
     gene            complement(7050..7943)
                     /locus_tag="VEL68_20890"
     CDS             complement(7050..7943)
                     /locus_tag="VEL68_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008232114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ38498.1"
                     /translation="MMNSESFKTPPGKGSPAGKFQAGSSGREFWKAGKSLLIFILAFL
                     YLVPVIVLFLTSFKTQIQIMEKGFGWFFMPTLMNYSNVLLEQGFLRYMTNSLIVGLAS
                     TFVTLIIGGFASYALVRFTFLGKGTISFGTLLLRMIPPAVLVVPIYVLWSYFGLADTR
                     LGLVIIYVGLNLPFTIWVLISFIADVPVELEEAAVVDGCGPWKIFFTVILPLIKPGLA
                     AAAIFTFRIAWNEFILALVLTNRMTRTLPVATTIYLTDTGVEWGNITAMGTLVALPAI
                     IFTFLAAKQLIAGLTAGAVKG"
     gene            complement(7968..>8590)
                     /locus_tag="VEL68_20895"
     CDS             complement(7968..>8590)
                     /locus_tag="VEL68_20895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ38499.1"
                     /translation="GLLIAFLLEKGIKGIVLLRTVFIVPIMIAPVVVGLLWRYLFDAN
                     FGLINYTVSLLGFEPKVWLGTPGLALPAVIITDIWQWTPFMFILFLAGLQSLPKDPVE
                     AAQVDGATTWQVIRQVKLPLLAPVIWVAVILRVIDAFRSLEVMFIMTFGGPGRETEVL
                     SLNIYKTAFMSQRLGLAAVNAIFLLAFILLLSMAFLIIARPALSKK"
BASE COUNT         1940 a         2458 c         2343 g         1849 t
ORIGIN      
        1 ggaccctttc catgagcgat cctggtaggc acctgtcacc tgcccctgta actccactcg
       61 attcacggat gcggcgaatt gagcccggtg aaaggaagga cttccatccg cctggaaccg
      121 tacagaagac aggtccacgg cctcggcctt gatattcccg gctcgcgcct ccagggacaa
      181 aacgggtgaa atcttggggc cgtattcttt gagctgaatt tcagcacgga ggtctccggc
      241 ccttgcttcc tgagtccata gctctttgct ctcggccacc atcgccccgc tcaggcggtc
      301 ttcctggtag cggaaccaac ctgccccgga tatctgacct ttggcctggg tgatgaactg
      361 ggaaaggtcg gtcactttcc cctccagagt gattctttcc tggagaatcc ctttccctcg
      421 gagatcgaaa ccccgcccgc gcaggcgaag gtgagcgatg ttcaggaggc ttccttccca
      481 gcggccctcc aatgcaccgg tcacggcctt atccagaaac cggctttcca ggaggttcgc
      541 gtcgaaggcg gcccccggct tcccggtttt gggccagaga agacggccgt ccaggttcaa
      601 attgatttct cctgatatat ccggagtcaa taacgcgggg ttcagctttc gcccctggag
      661 actcccctgc aaagagaatc cgttggccca ggagatcctc aggggacctt tcactgaacc
      721 gtcaagccat cttcccatga gggttgtcac ttccaaatat tccaggttcc cgcggaagac
      781 ggccgatgcc tgggctttct gccagccttt tgccagattg gctaccgtga ggtgccctcg
      841 gtatgtatca aggggacctt tgatctcgat ggttccggtg agattggaaa ggaggtccaa
      901 ttccggaacc agggtgatat ttgagaaatc ggctttcaag gagaaggtcg gcttctcccc
      961 aaaggcgatt tctccttccc cttgaatctt ccctttcgtc cctggctgaa ggagacggag
     1021 gttttggaac tgaagtacgg accgggtcaa ccccagattt ccttgcaggt ggaactcttg
     1081 cacagccttt cgccgcgcgg aaagggaaaa aaaccccttt gcctcttcct gggcccgaac
     1141 cggctccagc ccgagttgga cctggaaaga atcgagaccg gcaatttcgt cggccagggc
     1201 ggcctgcaaa tccagactga aggaaggtgt ggagaaaccc attttcatgg acccctcggc
     1261 cttaaccgaa ggaccttcca ggatgaagtc ttgaaccttc agaacttctc cgtcccattc
     1321 cagccgggcg gagagcgcat caaaaggtat ggggttttct tggagccgcc ggtagaaccc
     1381 cctttctact cgcagagtat ccacctgccc ctgcagccgg gaaagccaga aaggagttac
     1441 cggccagcca cgaaaggaaa tcgggccggt ctccgggcgg ttatctgcga cgcaaacccc
     1501 ctcgagggac agttcgcgaa taaggagttt tcggttccag agatccccgg gttcccagcg
     1561 gagatggaaa gagtcggcct gaagctcgcc ctggggccag cggattcgca accccctcat
     1621 cttcagttca tccttcactc gcccggaaat ttcccgggcc tcgatccgca ggggagtcaa
     1681 gagagaaatg gtcttcagga gaacccgggt gccttccgcg gagttcagag cccagaatcc
     1741 cacagaggcc aggcccccca gaatcagcac gagggagatg gaaatgatca ggagatattt
     1801 ccgactcttt ttcacagggc gaacccgatg gatatgtgga gctggaagcc ggggtgttcc
     1861 acgttgattt ggcgcgcgag gtccacccgg accggaccgg caggggtgta ataacggaac
     1921 cccaaccctg cgccctgggc cagctgcagg ttgtcaagat tgttgaaggc atttcccacg
     1981 tcgtagaagc ccgcgacact ccatttcctg gtgatagcgt actccagttc caggctcccc
     2041 acccagagat tttttccccc gatcacgttt ccgctcgaat ccttggggcc caaggactgg
     2101 tacgtgtacc cacggacgct gcggtcgccg ccggcataga agcggaggga ggggggcaga
     2161 tcggtgagag ggtccttttg ccaggtaacc cccacttggg agcggggaat cagggagaag
     2221 cccgctccca ggggaatgag aaaatcggca tttcccagga actgcagaaa attggtctcc
     2281 gcccccaaag cctgggtaga ccccctggtt tccagggcat aacgaaaacc cttgccgggc
     2341 cggattagat cgtccacccg cctttgcgaa aaacgcagcc cgggcatgaa aaggttggag
     2401 ctaccctcca ccccggcctc ggaaaaacgc tcctcccgga attggaggta ggccgaaccc
     2461 acggtcccgt acccgaagct gcgggcctgt tctacctcgg tggtgaagag aagattgtcg
     2521 tagggtttca aaagttcccg ctgcagtccc accttgaggt tggtccggtt gtcgacatgg
     2581 ccgctgccgg gaatggagta gtaggaggag atcgcctggc gtctttgggc gacggtcgca
     2641 tcggcgttga attcatggcc ccgcttgaaa gcgttcacgt cctgatagtg gagggatacc
     2701 tgagcaccgt aatcagtgga atatccgact ccaggacgaa gacgcttggg ggcggaaggc
     2761 tcgatatgaa ttcttaccgg aaccctaccc tcatgggctt cttccttatc cgcccggatg
     2821 ttcaccgaag agaaccggtc cgaattgatg agattcaact gggtctggta aattttggag
     2881 tatgaatagg gctggccggg cttgaagtcc agaaatcttt ccaaatagga gaccggatat
     2941 aattgggtcc cttcccagat cacttctccg aacagatact gggacccagt ctgcaggatc
     3001 agctcaatct cggctttgtg ctccccccgg tccacacgga tgacatggga agtatattcc
     3061 gaacccaggt atccctggtt caaggccttg gtccttaatt cctccttggc tttttcatac
     3121 ttcggctggt ttaacacgtc tccggctttc aggggaaagg cagctaccag ctgcttaagg
     3181 tcgggatttg tttccccagg cccggaaatg cggacaatca tggaagcgac ccgaatcggg
     3241 cttccgggaa tcaccttcac ccgcaggaac tcttggcctt cctccccttt ttcctgggcg
     3301 gatgaaacct gagcctcata gtagccgaag ggctcgaggg ctttctggac tttttctgaa
     3361 atctgatggc ggaagacctc cagtagcgcc cgatcgatga ccccctctcg gaccattccg
     3421 ggcgggaggg cgagggcggc ctgggcgttc ttcagggcat ccccttgaat tccttcgatg
     3481 accaccttga gagcctctgc agccatggca ttccagacga ggcatgcccc taaaatgaaa
     3541 aataagagag gaaatagtga tcgaaatctg tccggcaatc tttttatccg agcttcagac
     3601 ggttgagata ttttagcccg cccatcttca gcctaaatca ggccttgttt aaggatttta
     3661 ccgctttttc cgggggacag ataggctttt tgtgatttag aaaaggcagc aaaaagagcg
     3721 gatggccatc tgagaggcag gaggggaggg ctggggacaa ccccccccga gcccccagca
     3781 gtctgaggag agtttcgatt ttttaggccg cgatccagcc accatctacg atgagtatat
     3841 ggccatttac aaaattggaa gcctcggagg ccaggaagac gacgggaccg aataggtcct
     3901 ctaattcccc caaacgtctc aggggaattc ggccgaggac ccagtctctc ttttccggat
     3961 catccaaaat ggaccggttc atgggagtca gggtaaatac tggagcaatg gcattgacgt
     4021 tgatcccttt caccccccat tcgattgcca gggaacgggt gagttgattc acggcccctt
     4081 tgctcggtgc ataagctagg tcctctgccc ggccctggaa gctccgaacc gaggaaatat
     4141 tgatgatctt cccttttccc tgggtcagca tcacccgtcc cacatgcttg caggacagga
     4201 aaagcgattt gagattgaca tccatgaccc gctgccaggc ttcgacgtcg aactccgccg
     4261 agggctgcag gtgggagatc ccctgactgt tcaccagaat atcgatcctg cggaaacctt
     4321 cgagggcctg ggaaacagtt ctcttcacat tttcttccag ggtcatatcc gcttcgatcc
     4381 aaatgctttt tcgccccagt ttggagatgg ccgaagcggt ttcctcaagg tttttggctc
     4441 ccagcccgac cacgcagaca tctgctccgg cggcagcaag ggccaaagcc attccctgac
     4501 cgaggccctg acttccaccc actaccatgg ctgctctttg atccaaacga aaccggttca
     4561 aggtggaatg agccagattc acaattcgtt cgatttcaga cattatcatt cctcagctta
     4621 ggttacaaaa tgcaagtcag cgcgatgcgc tttgagctta cacgagagtc ttttcgtccc
     4681 ggatatcgaa gaggtggatg ttttccatgt tgggctgcag gcaaagcctc gcgtgcggct
     4741 tcgccgtagt gttgggctcg gcccgggcga tgagcgattg ctcggcggct ttcacatcca
     4801 ggtaaacctc gctccccagg ggctccatca cctccacagt cacatcgaag gtctccccgg
     4861 gagccgcaca aggggcctct ggcaaatcct cgggtcgaac cccaaagatc acccgcttct
     4921 ccgaataacc cttcaccttg gacgctttgt ccccggggat cttcaatttc atcccgggcc
     4981 cctgaaagtg aaccgctccc ccctcggaaa acaattcccc ttcgatgaag ttcatggccg
     5041 gactgccgat aaagccagcc acaaatttat tggccgggtg gaagtacaac tccaagggag
     5101 gccccacctg ctgcttcaac ccgtccttca tcaccacgat ccggtccccc atggtcatgg
     5161 cttccacctg gtcgtgggtg acgtagatga tcgtggtctg caaccgctca tggagcttct
     5221 tcaactccac ccgcatctgc acccgcaact tggcatccag gttggaaagc ggctcgtcga
     5281 agaggaaaac cttgggcttg cgcacaatgg cccgccccac ggctacccgc tgacgctgcc
     5341 cacccgaaag agccttcggc ttgcggtcca aaagctcctt gatgttcagg atttccgcag
     5401 catccttcac ccgctggtcg atctcctcct tggggaactt gcgcaacgtc aacccgaagg
     5461 ccatgttctg gtaaaccgtc atgtgcgggt acaaggcata gttctggaag accatggcaa
     5521 tgtcccggtc cttggggggc aggttattga ccaccttgtc gtcgataagg atatcccccg
     5581 aggtgatgtc ctccagaccc gccaccatgc gcagggtggt ggtcttcccg cagccgctgg
     5641 gaccgaccag gacgacaaac tccttgtccg gaatctccag gtcgaaatcc ctcacggctt
     5701 tgacttctcc aaaatgcttg ttcagtttct ccatcagaac tttagccatg ccttctctcc
     5761 tcctgtcaaa agtggttcat ggtcgaaata agcacttcct atttcgaata ttttccatat
     5821 tccagagccg ctgcatacaa agcacagata ttttccggct tgacctccgg gcggaggttg
     5881 tgcacggcgc ctaggacaaa tccgccatct tgagcgaggt cttcaatgcg acggcgcact
     5941 tcgttttgca cctcgagcgg agttccaaag ggaagaatcc actgagtatc aattcccccc
     6001 cagaaggtta agtgccggcc atattttgct tttagtctct tcgtatctcc catccccttg
     6061 gccgagacct ggaccgggtt aatggcgtca attcccaagt cgatcaaatc cgggaggagg
     6121 ggctccacgc ccccgcagca gtggtacagc actttggcat ccccgaattc ctctttgagg
     6181 acccgcatga ttttccgatg acggggtttg aagagctggc ggtacttgtc cggagagtag
     6241 gttatccccc tctgatggga gaggtcgtcg ccgatcacga tcacatccat gtgagggtcg
     6301 atttcttcca gaagatggcg ggtcatctcg agctggatcc ccaggatttt atccatcatg
     6361 tattcaagga gagaggggtt caggacgctg tccatcatga attgctccaa accccgcatc
     6421 ccctgagttt gggtcatgac catcaggggg gcgcttgcca tgactccata tccctcttcc
     6481 tgataacgct tggcgatctc cttaaggccc cggtaccggc tggggtccgt gggatcgggc
     6541 cagggatgtt tctcgacctc cttcggggtg gtgacccggt gtaaaggcgc ttcgcgctcc
     6601 acgacgaaat gcgtctgggc ttcctcattc cactgataga caacgcccca ctcatctaca
     6661 attttcccct cgggcgtcga gtgaagctcg aattcagcgg gtggattagc ggtcagcatc
     6721 ttcacgtcga ccccgaacct ccggacgatc tcttcatccg ggtaagcggt aagaaagatg
     6781 gggctcatca tccgggttgg agactgaata cccagatggg ctttgaggtt ttcatgggcc
     6841 cgagcagaaa tagtgctggt gggcgtaccg cccaggtcga tgggcacccg atcaggctcc
     6901 cggtggttta aggccactaa aagcctctct ctggagttca atgagaagct tctttctcct
     6961 gcaaaatcca gaaagccgaa cgcaggatgg gaaaaaacca aattaaaaag agcattccgc
     7021 aatccgaatt ccggaattga ttggccgacc taccccttga ccgctccggc agtaaggccg
     7081 gcaattaact gcttggccgc gaggaaagtg aagatgatcg ccggcaaagc cacaagggtt
     7141 cccatggccg taatattacc ccattctacc ccggtgtccg tcaggtaaat ggttgtggca
     7201 accgggaggg tacgagtcat gcggttggtc aaaaccagag caagaatgaa ctcgttccac
     7261 gcgattcgga aggtgaaaat ggcagctgct gccaagccgg gtttgatcag gggtagaatg
     7321 accgtaaaga agatcttcca gggaccgcag ccatccacga ccgccgcctc ctccagctcc
     7381 accggcacat cggcaatgaa actgatgagc acccagatcg taaaggggag attcagaccg
     7441 acatagatga tgaccaatcc cagccgggtg tcggccaggc caaaataaga ccagagaacg
     7501 tagatcggaa ccaccagcac cgcgggggga atcatccgca gaagcaaggt accgaaggaa
     7561 atagtgcctt ttccgagaaa ggtgaagcga accagggcgt acgatgcgaa tcccccgatg
     7621 atcaaggtga cgaaagtcga cgccaaccct acgatcaggc tgttggtcat gtaccggagg
     7681 aagccctgtt ccagaaggac gttcgaatag ttcatcaggg tgggcatgaa aaaccagccg
     7741 aatcccttct ccatgatttg aatctgggtc ttaaaagagg tcaggaataa gacgatgacc
     7801 ggaacgagat agaggaaagc aaggatgaag atcaggagac tttttccggc tttccagaat
     7861 tcacggccag acgaaccggc ctggaatttc ccggccggcg agccttttcc cggcggggtt
     7921 ttgaaagatt cactgttcat catttcgcac tccctctcct cttcccgcta tttcttggat
     7981 aaggcaggcc gggcaatgat caaaaaggcc atgctcaaga gtaagataaa ggccaggaga
     8041 aatatggcgt tgaccgcggc caggcccaaa cgctggctca tgaaggcggt tttataaata
     8101 tttagggaca gaacttcggt ttccctccct gggcccccga aggtcatgat gaacatgact
     8161 tccaagctgc ggaaggcatc gatgacgcgg agaatcaccg ccacccaaat caccggggct
     8221 aagagaggaa gcttgacctg gcggataacc tgccaggtcg tcgcgccgtc gacctgggcg
     8281 gcttcaaccg ggtctttcgg caaactctgc aacccggcca gaaataggat aaacataaag
     8341 ggagtccact gccagatgtc ggtgatgata accgcaggga gagccagacc cggagttccc
     8401 agccagactt tcggttcgaa tcccaaaagg gagacggtat agttgatcaa accgaaattg
     8461 gcatcgaaca agtatcgcca gagcaaaccg accacgaccg gggcaatcat gatgggaacg
     8521 ataaaaacgg tccggaggag gacgatgccc tttatgcctt tctcgaggag gaaggcgatc
     8581 aagaggccaa
//
LOCUS       DASPRR010000568         8688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2388203, whole genome shotgun sequence.
ACCESSION   DASPRR010000568 DASPRR010000000
VERSION     DASPRR010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388203"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VEL68_20900"
     CDS             complement(<1..230)
                     /locus_tag="VEL68_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38500.1"
                     /translation="MMSWKYTLPAFVVPLFIGVGILAAGICGRLMRKTSGVERILLIV
                     AGFILVYPNPIADLLGLSLAGGVAFRQWQTRP"
     gene            complement(538..711)
                     /locus_tag="VEL68_20905"
     CDS             complement(538..711)
                     /locus_tag="VEL68_20905"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXZ38501.1"
                     /translation="MKFLERIFNNQKKADKKESLGRNDLCWCGSGKKYKRCHLDSDAH
                     KQRMRNGLGKKPK"
     gene            complement(793..1137)
                     /locus_tag="VEL68_20910"
     CDS             complement(793..1137)
                     /locus_tag="VEL68_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXZ38502.1"
                     /translation="MNKNLYIGNLSMKVTEEDLKANFSEVGKVVSVNIIKDRYTGISR
                     GFGFVEMETEEAAQEAIRRFSGGQLLGNTITVSEARPKKEGGGGGPRREGGRGGGGFR
                     GGRGGGGGGRRY"
     gene            1461..1841
                     /locus_tag="VEL68_20915"
     CDS             1461..1841
                     /locus_tag="VEL68_20915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ38503.1"
                     /translation="MHTRFVIQQHHASHLHFDFRLEMGGILRSWAVPKGPSMDPTEKR
                     LAVEVEDHPLEYIDFEGTIPEGEYGAGRVLIWDRGTYRLLERRNDKIIFFLEGEKLRG
                     NFALVRLKRSKTGRDWLLIKQKDS"
     gene            complement(1985..3481)
                     /locus_tag="VEL68_20920"
     CDS             complement(1985..3481)
                     /locus_tag="VEL68_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ38504.1"
                     /translation="MEIIGPLWTGLATTLAPGNLLVVFLGVVIGTVIGVLPGLGPTAS
                     IALLLPATVGMDMISSLIFLAGIYYGAMYGGSTTSILVNIPGEAASVITCIDGYQMAR
                     QGRAGAALGIAAFGSFIGGTLGVVAVMLISPFLAEAALKFGAPEMFALLFFAFTCIGT
                     LVGKSVARGLLMAALGLLLGVVGTDDIHGVDRFTFGTLYLRDGIGLVPLAMGLFGLGE
                     IFYNIEQREKTGSVLAVPKNLLPTRKDWKESFGSILRGSGLGFGIGVLPGGMPAVASL
                     MSYAVEKRVSKRPERFGTGAIEGVAGPETANNAATAGGFVPLLTLGIPTNSVLALLLA
                     ALMLHGVTPGPLLLLRWPHVFWGVIASMYVGNIMLLILNLPLIGIFVQLLRIPYAILS
                     PLIIMFVLLGAYALNNSIADVFMTLFFGVLGYLLRRFAFEPAPLVLAFVLGPMMEVNL
                     SKALIMFGTGNPLYFFTRPISGVLLTLALALLSFPLVRGLAKKLSPKA"
     gene            complement(3492..3947)
                     /locus_tag="VEL68_20925"
     CDS             complement(3492..3947)
                     /locus_tag="VEL68_20925"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ38505.1"
                     /translation="MNRVDRGVGVAALLGGVLLAIAGWRLDPGTSKLPGPGFFPLLIA
                     CTMSGLGVWLLLRPGSEEKSVSPESSRWSAFAIALASILGYTMVLNELGYLVSTFALL
                     VVQLRWVETQRWSTSFWTAAIASAVSLVVFRVLLKVPLPAGFFPLPKGW"
     gene            complement(3959..4924)
                     /locus_tag="VEL68_20930"
     CDS             complement(3959..4924)
                     /locus_tag="VEL68_20930"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ38506.1"
                     /translation="MERKGFRSWTGLFFLPALFLAVGYAQAQDYPSKPIEVIVGYPPG
                     GGTDMIARAVADVAHKYVGQPLVVINKTGATGTIAAQSVAVAKPDGYMLLVAGGSETI
                     SVPHFKSLPYDAINDFVPIIRLMIERVGFYVRTDSPWKTMPEFVADAKQNPEKYTYAT
                     SGIGGLHHSTVLVTEKRTGMRLRHVPHKGGAETLAALAGGHVNVAMASPNEAYALVQG
                     GRIRPLAHSSLARSPVEPNTPTLRELGYDVYIENQKGFVFPKNTPKPIVQKLHDSLKK
                     VFDDPQFKSSAQKLQVELAYLSGEDFHKSMKAMYEQVGASLKKQP"
     gene            5206..5925
                     /locus_tag="VEL68_20935"
     CDS             5206..5925
                     /locus_tag="VEL68_20935"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXZ38507.1"
                     /translation="MIPAVQSTRVVQEMEAAILSGALKPRERLVEMDLISRYGVSRTV
                     IREALKRLESRGLIRTAPFRGAAVADLTVEEIEELYFARAAIEKTAAGLVLDHIRPEE
                     VQRLKTLLRDLESHLRRKTPQMIEKDLEFHRAVYQTCRNRYLCEVIDFLRTKAHIVGY
                     NAWSLPQRIEQSILEHREILRAIEEGHRPRLEKLVVKHITFSKRSYQTQLKGMDRTNP
                     GVKKPLPMGMRKKTRPEKSKE"
     gene            5946..6185
                     /locus_tag="VEL68_20940"
     CDS             5946..6185
                     /locus_tag="VEL68_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXZ38508.1"
                     /translation="MAKVRIYRELCKGVEECGICVSVCPKKLFQPSPALNPKGYRPPE
                     MKNEAVCTGCENCMIYCPDLAIAVKRDKTAKGAKK"
     gene            6182..7336
                     /locus_tag="VEL68_20945"
     CDS             6182..7336
                     /locus_tag="VEL68_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ38509.1"
                     /translation="MKGTRVQTGKYFMMGNIACAEGALAAGCEFAAGYPITPATEIAN
                     RLAERLPQVGGMFLQMEDEISSIAAIIGASWTGKKVLTATSGPGISLMLENIGFAIGV
                     ETPCVIVNVQRGAPTTGMPTAGVPADMVQPKRGSHGDYEIIALCPASPQEMFEHTILA
                     FNFAEKYRVPVFILSDAFVGHMREEVVIPEVEKIEIVNRKVPEIGTDPQKVKGFLDED
                     VAPMFIFGKGFRSHVTSSCHDEYGKRNLSDLTALDNFIRKLSDKILKHKEDIVRVERD
                     YVGAEVVLVSYGAVSRAAKMAAMRAREQGLPVGTLRLITPWPFPGEEIEEMARKAKKV
                     IVLENNLGQLYPFIKAEAACHADVSFLPPRVIGEIQDPEAILSRIREVMK"
     gene            7333..8166
                     /locus_tag="VEL68_20950"
     CDS             7333..8166
                     /locus_tag="VEL68_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ38510.1"
                     /translation="MNEIVHPLHRFIRPYVTSTTNCPGCGNGIAAQAILRAVEELGMT
                     LDDFIFVTGIGCSAWIPSPFFNVDVLHTTHGRPIAFALGIKMGLPEKKVMVTSGDGDL
                     AAIGGNHLIHNARRNVEMVVICLNNGIYGMTGGQAAPTTPMGLKTATTPYGTFENPFD
                     ISELVIAAGATFVARWTTHHARQLTASIKKAIQKKGFSFIEVITQCPVQYGKKTGSGS
                     AVQMMKEYKEKSILVKEAAKLPKDELHGRIVVGELVDKEAPEMLEEINRMKKKVAGEK
                     A"
     gene            8163..>8688
                     /locus_tag="VEL68_20955"
     CDS             8163..>8688
                     /locus_tag="VEL68_20955"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ38511.1"
                     /translation="MNRTEILVGGVGGQGVILAGILLGAAATLFDGKKAVQTQAYSSE
                     QRGGMARAEVIISDEPVTDPQVRRPDVLMALAQDAVNRHLKKIKPGGLLVVDSDLAQE
                     VEKGNFEVLSIPATSIADKEMGSVVVANLILLGGLLKKTAVLSVGAMEKAIEANVPPK
                     AKEMNLKAFRRGLEF"
BASE COUNT         2056 a         2466 c         2510 g         1656 t
ORIGIN      
        1 ctcggtcgag tctgccattg cctgaaagcc actccgccgg cgagagacag gcccagcaaa
       61 tccgctatcg gattagggta caccagaata aaccctgcga caatgagcaa aattcgctcg
      121 actcccgatg tcttccgcat cagccggccg caaatccccg cagccagaat cccgactccg
      181 atgaacaagg gcacgacgaa agcgggaagg gtgtacttcc aggacatcat ggtggtcttg
      241 tagggatcgc ccccggtaag ggcggccgcg gcaaaggcat tgagagccgt gggaagggta
      301 acctcggcca ggggcgattc atgaattacc cctactcatc cggggacgat ctcaggattt
      361 cccgggaggg gtctcaattc tctcagggtc atcgaaggac agaggggttt tgatcagggg
      421 agaaaacaaa aaagcacagc ccccaagccc agatgcaatg aaaacccaga aaatgattgc
      481 tgtcagtcgc ctgggggcca tgccctaaga gaagaagatg gagtttttct gccgttccta
      541 cttaggcttc ttgcccagcc catttctcat acgctgttta tgggcatcgg aatccagatg
      601 gcagcgtttg tatttctttc cgctaccgca ccagcaaagg tcgttccgac ctaagctttc
      661 cttcttgtcg gctttttttt gattattgaa aattcgttcc aaaaatttca tctctacctt
      721 cacccgattt cacttgaatc cctggatcgc aatcggcatt ccgcaatccg cttccggatt
      781 tccgcgattg aactaatacc tgcgaccccc gcctccgcca ccccggccgc ctcggaaccc
      841 gccgccgccc cgcccgcctt cccgcctggg cccgccgcca ccgccttctt ttttgggccg
      901 ggcttcgcta actgttatgg tgttgcctag aagctgtcct ccgctgaacc ttcggatggc
      961 ctcctgggcc gcttcttcgg tctccatttc cacgaatccg aatcccctgg aaatcccagt
     1021 gtacctatct ttgatgatgt ttaccgagac gactttaccc acttcggaga aattcgcttt
     1081 caaatcctct tcggtgacct tcatggacaa atttccgata taaagattct tgttcatacc
     1141 ggccttccct cctttttcgt tatccctctt tcggatttga agcgaaaaat cttcaaggga
     1201 tttcatcctc cgaaggagat cgctctcgga atccctcggc ctcaaaaaaa aagccgccct
     1261 tggcttaaag agccaggcgg cttgcctttt cgatacctaa gctctttaca agacttatta
     1321 ataaggatag attttacagg aatataagtc aagctctatt tcgaaaacag ctggtttgag
     1381 atcgaaattc cctttgaaaa ttccctttct agcctatctc atcggcaagt tgcagaggcc
     1441 agaccatcat tgaggtgatt ttgcacactc gattcgtcat tcaacaacac catgcctccc
     1501 acctccattt cgacttccgc ctggagatgg ggggaattct gcgttcatgg gcagtcccca
     1561 aggggccatc catggacccg acggaaaaaa gactggcggt ggaagtggag gaccaccccc
     1621 tggagtacat agattttgaa ggaacgattc cggaagggga gtatggggcc ggtcgggtct
     1681 tgatctggga ccgggggacg taccgactcc tggaaaggag gaatgataaa atcatattct
     1741 ttctggaagg agagaaactc cgaggaaatt ttgccctggt ccgacttaaa aggagcaaga
     1801 cgggaaggga ttggctgctg attaaacaga aggatagtta gggggatccc tcctcgcttc
     1861 gattttaatt tttcccacct ccatcatcgc cttgaagtaa acaggttgat tttcaagaag
     1921 aaagaggcga aagatcgacg gaacccatga ccgagcggat aagtcccaag cctcttggga
     1981 gtattcatgc tttcggggaa agttttttcg ccagcccgcg cacaagaggg aaagaaagga
     2041 gtgctagggc cagggtcaga agaacccccg aaatcgggcg ggtgaagaag tacagcggat
     2101 ttcccgtccc gaacatgatc agggccttac tgagattgac ttccatcatg ggtcccagca
     2161 caaaagccaa gacgaggggg gcgggttcaa aagcgaaccg cctcaggagg taacccaaaa
     2221 ctccgaagaa gagggtcatg aagacatcgg cgatactgtt gttcagggca tacgccccca
     2281 ggaggacgaa catgatgatg aggggggaga gaatcgcata aggaatacgc agcagctgga
     2341 cgaaaatccc gatgagcggc aaattcagga tcaagagcat gatgttcccg acgtacatgc
     2401 tggcgatgac tccccagaaa acatggggcc acctgagaag aagcagcgga cccggcgtca
     2461 cgccatggag catgagggcc gcaagcaaga gcgccaggac cgaattggtg gggatgccca
     2521 gggtgagcaa aggaacgaag ccgcctgcgg tagcggcatt gttggccgtt tccggacccg
     2581 ccaccccttc gatggccccg gtaccgaacc gctccgggcg cttggaaacc cgtttctcga
     2641 cggcatagct catgagggaa gcaacggctg gcattccgcc cgggagcacc ccgatgccga
     2701 atccgaggcc gctgccccgg aggatcgacc cgaaagactc cttccaatcc ttccgcgtgg
     2761 gaaggagatt cttcgggacc gccaagaccg aaccggtttt ctcccgctgc tcgatgttgt
     2821 aaaaaatctc ccctaggccg aagaggccca tggccagcgg gaccaggccg atgccgtcgc
     2881 gcaggtaaag ggttccgaag gtaaagcggt ccacgccatg gatatcatcc gttcccacca
     2941 cgcccaggag caggcccagg gcggccatga gaagtccccg ggcgaccgat ttgcccacca
     3001 gggtgccgat gcaggtaaag gcgaaaaata gaagcgcgaa catctccgga gcgccaaact
     3061 ttagagccgc ctctgcaagg aaaggggaaa tgagcatgac cgccaccact cccaaggtcc
     3121 ctccaatgaa agacccgaag gcggcaatgc cgagggcggc cccggctcgg ccctggcgcg
     3181 ccatctggta gccgtcgata caggtgatca ccgaggcggc ttcgcccggg atgttcacca
     3241 gaatggaagt tgttgatccc ccgtacattg ccccgtaata aatcccggcc aggaaaatga
     3301 gcgaagaaat catatccatc cccaccgttg ccgggaggag cagggctatg gaggccgtgg
     3361 ggccgagccc gggcagaacg ccgatcacgg tcccgatcac cactcccaga aatacgacca
     3421 ataaatttcc cggggccaaa gtggtggcca aaccggtcca tagagggccg ataatttcca
     3481 tccgttgtct ttcaccaccc cttgggcaag gggaaaaatc ccgccggcaa gggcactttt
     3541 agcagcaccc ggaatacgac cagggagacc gccgaggcaa tcgccgccgt ccagaaagag
     3601 gtcgaccagc gctgtgtttc cacccagcga agctggacga caagaagcgc gaaggtggaa
     3661 acgaggtagc ccagctcgtt taggaccatt gtgtaaccta agatggaagc cagagcgatg
     3721 gcgaaggcgc tccatcggga cgactcgggg gaaacagatt tttcctctga tcccggacga
     3781 aggagaagcc acacccccag gccgctcatg gtgcaggcga tgagcagggg aaagaacccc
     3841 ggtcccggaa gtttggaagt ccccgggtct agccgccacc ctgcgatcgc cagcaacact
     3901 ccaccgagaa gggcggcgac gccgactcct ctatccaccc ggttcatcgt ttctcctcct
     3961 aaggctgctt tttcagggag gcccccacct gctcatacat ggccttcatg ctcttgtgga
     4021 aatcctcccc gctgaggtaa gccagttcta cctgaagctt ctgggcgctg gacttaaact
     4081 gagggtcatc gaaaactttt ttcaggctgt cgtggagttt ctggacgatc ggcttcgggg
     4141 tatttttggg aaacacaaag cccttctggt tctcgatgta gacgtcgtaa cccaactccc
     4201 gcagggtggg agtattcggc tccaccggag aacgggccag ggaggaatgg gccaaaggac
     4261 gaatccttcc cccctggacc agggcatagg cctcgttggg cgaggccatg gccacattca
     4321 catgacctcc agccagagcg gcgagggttt ctgctcctcc cttgtggggg acatggcgga
     4381 ggcgcatccc cgtccgcttt tccgtcacca atacggtgga gtgatgcaag ccgccgatcc
     4441 ccgaagtggc gtaagtatac ttctcgggat tctgtttggc gtcagccacg aactccggca
     4501 tggttttcca ggggctgtcg gtccggacgt aaaagcccac gcgttcgatc ataagccgaa
     4561 tgatggggac gaaatcgttg atggcatcgt agggaaggct cttgaagtgg ggcacggaga
     4621 tggtttcgct ccctccggcc acgagcagca tataaccgtc cggtttcgcg acagcgacgg
     4681 attgagccgc aatggttccg gtggcccccg ttttattgat gaccacaagg ggctggccca
     4741 catatttgtg ggccacatca gccaccgccc gggcgatcat gtccgtcccc ccgccgggag
     4801 ggtacccgac gatcacttca atgggtttgg aaggataatc ctgggcctga gcataaccga
     4861 cggccagaaa gagagccgga agaaaaaaca atccagtcca gctgcgaaat cctttccttt
     4921 ccatgccttt tctcctttct cggctggatt tctttaccta gatcaagatt aagaaggaaa
     4981 aattccttcg ctgcagggaa ggatgcagga tggcatcccc aaggcaaaac gtgcgcccgt
     5041 gtcaaggccg ctcgcgaaat tccttgaaca gacgaaaaat ataataggaa acggagggat
     5101 gtcaaggaaa aggaagacct cccaaaaaag aaaaactgtt tgacaccctc ctccggaaat
     5161 tgtatacatg attgtattta aaaaacgcac aggagcccgg agcccttgat tcccgcagtt
     5221 caatccaccc gagtcgtgca ggagatggag gcggccatcc tttccggggc gttgaagccc
     5281 cgggagcgac tggtggagat ggacctcatc tcccgttatg gcgtcagccg gacagtcatc
     5341 cgagaggctc tgaagcgcct ggaatcccgg ggtctcatcc ggacggcgcc tttccgcggg
     5401 gctgcggtag ccgacctgac ggtggaggag atcgaagagc tctatttcgc ccgggcggcc
     5461 atcgagaaga ccgccgccgg gctggtcctg gatcacatcc gtccggaaga ggtccagagg
     5521 ctgaaaactc tgctccgcga tttggaaagc cacctgcggc gcaagacccc ccagatgatc
     5581 gaaaaggacc tggagttcca ccgggccgtc tatcagacct gccggaaccg gtacctctgc
     5641 gaggtcatcg actttctgcg aaccaaggcg catatcgtgg ggtacaacgc ctggtccctg
     5701 ccccagcgca tcgagcagtc gatcctggag caccgggaga ttttgcgggc catcgaagaa
     5761 ggtcaccgcc cccggctgga gaaactggtg gtgaaacata tcaccttctc caagagaagc
     5821 tatcagaccc agctgaaagg gatggatcgg acgaatcctg gagtgaagaa acctctcccg
     5881 atgggaatga ggaaaaagac gaggccggaa aaatcgaagg aataaatggt aagggaggaa
     5941 acacgttggc caaggttcga atctaccgcg aactctgcaa aggggtggag gagtgcggca
     6001 tctgcgtctc cgtctgcccc aagaagcttt ttcaaccgtc ccccgctttg aaccccaaag
     6061 ggtaccggcc tccggaaatg aagaacgagg ccgtctgcac gggctgcgag aactgcatga
     6121 tctactgccc ggacctggcc attgcggtga aaagggataa gaccgcgaag ggggccaaga
     6181 aatgaagggg acacgggttc aaacgggaaa atatttcatg atgggaaaca tagcctgtgc
     6241 cgaaggagcc ctcgccgccg ggtgcgaatt cgccgcgggg tatccgatca ccccggccac
     6301 ggagatcgcc aaccgtttgg ccgagaggct cccccaggtg gggggcatgt ttctgcagat
     6361 ggaggacgag atcagctcca tcgccgccat catcggcgcc tcctggaccg gaaagaaagt
     6421 cctgaccgcc acctcaggtc cgggaatcag cctcatgctg gaaaatatcg gctttgccat
     6481 cggggtggag acaccctgcg tgatcgtgaa tgtgcagcgc ggagcgccga ccacgggcat
     6541 gcccaccgcc ggggttccgg cggacatggt ccagcccaag cgggggtccc atggggacta
     6601 cgagattatc gccctctgtc cggcatcccc ccaggagatg ttcgaacata cgattctggc
     6661 cttcaacttt gccgaaaaat accgcgtgcc cgtatttatc ctttcggatg cttttgtggg
     6721 acacatgcgg gaagaggtgg tcatccccga ggtcgagaag atcgagattg tcaaccgcaa
     6781 ggtccccgag atcggaaccg acccccagaa ggtcaaaggg ttcctggatg aagatgtggc
     6841 ccccatgttc atcttcggga aaggcttccg ttcccacgta actagttcgt gccacgatga
     6901 gtatggaaag cggaacctga gcgacctcac cgccctcgac aacttcatac ggaaactttc
     6961 cgataagatt ctgaagcaca aggaagacat cgtgagggtc gagcgggact acgtgggagc
     7021 cgaggtggtc ctggtctcct acggcgccgt gtcccgggcg gccaaaatgg cggcaatgcg
     7081 ggctcgggaa caggggttgc cggtggggac tctccggttg attaccccct ggccctttcc
     7141 cggtgaagag atcgaagaaa tggcccggaa ggcaaagaag gtgatcgtcc tggaaaacaa
     7201 cctggggcag ctttatccct tcatcaaagc cgaggccgca tgccacgcgg acgtttcttt
     7261 cctaccgccc cgggtcatcg gagaaatcca ggacccggag gccatcctga gtcgaattcg
     7321 agaggtaatg aaatgaacga aatcgtccat cccctccatc gcttcattcg tccctacgtg
     7381 acttccacca ccaactgccc gggctgtggg aacgggattg ccgcccaggc catcctgcgg
     7441 gcggtcgaag agctgggcat gaccctggac gatttcatct tcgtaaccgg gatcgggtgc
     7501 tcggcctgga tccccagccc ctttttcaac gtggatgtcc tccataccac ccacggccgc
     7561 cctatcgctt tcgccctggg gatcaagatg ggcctgccgg agaagaaggt catggtcacc
     7621 agcggggacg gagacctggc ggccatcggc ggcaaccacc tgatccacaa cgcccggcgg
     7681 aatgtggaaa tggtggtcat ctgcctcaac aacgggatct atggcatgac cggagggcaa
     7741 gccgccccga ccacgcccat gggattgaag accgccacca ctccttacgg gaccttcgag
     7801 aatcccttcg acatttccga gctggtcatc gccgcgggag ccaccttcgt ggcccggtgg
     7861 acgacgcatc acgcccggca gctgaccgct tcgattaaaa aggcgattca gaagaaaggc
     7921 ttttccttca tcgaagtcat cacccagtgc ccggtccagt acggaaagaa gaccgggtcc
     7981 ggcagcgcgg tgcagatgat gaaggagtat aaggaaaaat ccatcctggt gaaagaggcg
     8041 gccaagcttc caaaggatga gttgcatggg aggatcgtcg tcggggagct ggtggacaaa
     8101 gaggcgccgg aaatgctcga ggaaatcaat aggatgaaga aaaaggttgc gggagagaag
     8161 gcatgaaccg gaccgaaatt ctggtgggcg gggtaggggg acaaggggtt attctggccg
     8221 ggattttgct cggcgcggca gccacccttt ttgacgggaa gaaagccgtg cagacgcagg
     8281 cctacagctc ggagcagaga ggcgggatgg cccgagccga ggttatcata tccgacgaac
     8341 cggtcaccga cccccaggtc cgcagaccgg atgttctcat ggccctggcc caagacgcgg
     8401 tcaaccggca tctgaagaag atcaagcccg gcgggcttct ggtcgtggac tcggacctgg
     8461 cgcaggaggt ggaaaagggg aatttcgagg tcctatccat tcccgccacc agcattgccg
     8521 acaaggaaat gggcagcgtg gttgtggcca acctgatcct gctgggcgga ctgctcaaaa
     8581 agacggcggt cctttcggtc ggggccatgg aaaaagccat cgaggccaac gtcccgccca
     8641 aggccaagga gatgaacctc aaagctttcc gaagaggact ggaatttt
//
LOCUS       DASPRR010000569         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_620232, whole genome shotgun sequence.
ACCESSION   DASPRR010000569 DASPRR010000000
VERSION     DASPRR010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620232"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..475)
                     /locus_tag="VEL68_20960"
     CDS             complement(344..475)
                     /locus_tag="VEL68_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38512.1"
                     /translation="MQKGRGGSRSAPARFRASRPSPTYKNFVNRCLCEKGYDPIGWE"
     gene            complement(524..1876)
                     /locus_tag="VEL68_20965"
     CDS             complement(524..1876)
                     /locus_tag="VEL68_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HXZ38513.1"
                     /translation="MEDMKVYLTEKEMPRQWYNVQADLPKPLPPPLHPGTLKPLGPQD
                     LAPIFPMGLIEQEVSQQRWIDIPEDVISIYKLWRPTPLCRAKRLEVALKTPARIYYKN
                     EGVSAAGSHKPNTAVAQAYYNKKEGVKRLSTETGAGQWGSALAFGCNVFGLECKVYMV
                     KVSYQQKPYRRMMMETWGASVTPSPSDQTDAGRMILAKDPNSPGSLGIAISEAVNDAV
                     KREDTRYSLGSVLNHVILHQTVVGLETKAQLKKIGVEPDVLIGCVGGGSNFSGFCFPF
                     FPEKKQGKKVRFVAVEPTACPTLTKGLYRYDFGDTAQMAPIVKMYTLGHTFVPPGIHA
                     GGLRYHGDAPLLCLLYDEGFVEAVAYPQNPVFEAAMLFARTEGTIPAPETAHAIRAAI
                     DEAIQCREKKEEKVIVFNFSGHGHFDLAAYDMFLSKKLEDYEYPEEKIKEALKDLPKV
                     "
     gene            complement(2093..>2276)
                     /locus_tag="VEL68_20970"
     CDS             complement(2093..>2276)
                     /locus_tag="VEL68_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38514.1"
                     /translation="ANAMKKAGTDNEKLRDAIEATKGYVGISGTYNLTAEDHNGLGTD
                     SMVMVQISGGQWKKLD"
BASE COUNT          450 a          662 c          597 g          567 t
ORIGIN      
        1 gagggatgac cgctcctctg ctaggaaagg ttgcggacat ttatggaatc tggactgctt
       61 tagcagccat tctttttgtt cccttattaa gcgccggatt tggcctcacc ttgaaacccg
      121 ccaaaaccag gtaggaacat ccaacacgag atcatgttct caatcactta gatcctttca
      181 ggcctcaccc cattccaaat atttaatact tatccaattt accaacagat tactaaaaga
      241 agtcatcaca atttttggga atttatcgct aaacagtttc aggaagtaat gatttcctct
      301 ccgtccttac ggatgctgcc aagaaaagtt ccttctacgg tgcttactcc cagccgatgg
      361 ggtcgtagcc cttctcgcac aggcagcggt tgacgaagtt cttgtaggtg ggagacgggc
      421 gggaggcccg aaagcgcgca ggggcgcttc gcgagccgcc cctgcctttt tgcacctctc
      481 tttaaacctc gttcctcaga cctcgaacct cgggcctttc tttttatacc ttcggcagat
      541 ccttcagcgc ctccttgatc ttttcctccg gatattcgta gtcttccagc tttttcgaca
      601 ggaacatgtc ataagcggcg aggtcgaagt ggccgtggcc gctgaagttg aagacaatga
      661 ctttctcctc cttcttctcc cggcattgga tggcctcgtc gatggccgcc cggatggcat
      721 gagccgtttc cggagccggg atggttcctt ccgtccgggc aaagagcatg gcggcttcga
      781 agaccgggtt ctgcgggtag gctacggctt ccacgaaccc ctcatcgtaa agaagacaga
      841 gcaagggagc atctccgtgg taacggagtc ccccggcatg aatcccgggg ggaacgaagg
      901 tatgtcccaa ggtgtacatc ttcacgatgg gggccatctg ggccgtgtcc ccgaagtcgt
      961 agcgatacaa ccccttggtg agggtggggc aggccgtcgg ttccacggct acaaaacgga
     1021 cctttttccc ctgctttttt tcggggaaga agggaaagca gaatccggag aaattgcttc
     1081 cccctcccac gcacccgatg aggacatcgg gttccacgcc gatcttcttc agctgagctt
     1141 tggtctccag cccgacaacc gtctgatgga gaatgacatg gttgagcacg ctgcccaggg
     1201 aataccgtgt atcttcccgt ttgaccgcgt cattcaccgc ttcactgatg gcgattccca
     1261 ggctccccgg cgagtttggg tccttggcca ggatcatccg gcctgcgtcc gtttgatcgc
     1321 tcgggctggg ggtcaccgag gcgccccagg tttccatcat catccggcgg tagggtttct
     1381 gctggtagct aaccttgacc atgtaaacct tgcactccag gccgaagaca ttgcacccga
     1441 aggccagggc actgccccat tggccggcgc cggtttccgt gctaaggcgt ttcacccctt
     1501 ccttcttgtt gtagtatgcc tgggctacgg cagtattcgg cttgtggctc cccgccgcgc
     1561 tcactccttc attcttgtaa tagattcgtg ccggggtctt cagcgctacc tccagccttt
     1621 tagcccggca gagaggggtg ggtctccaca atttgtaaat ggaaattaca tcctcgggga
     1681 tatcgatcca gcgctgctgg ctcacttctt gttcgatcag gcccatggga aagatgggag
     1741 ccaggtcctg cggccccagg ggtttcaaag tgcccggatg gagcgggggc ggcaggggct
     1801 tgggcaggtc cgcctggacg ttgtaccatt gccggggcat ctctttttca gtcaaataaa
     1861 ccttcatgtc ttccattcaa ccctccatat tttgttttga acgcagatga acgcagattt
     1921 tcaggatgtt aaaaacaact atgggacaca gatgaacaca gcaccgcaca cccgcaacca
     1981 aaatcacccc ctccctaccc tcccccctcg agggggaggg ttagggtggg ggagagcctt
     2041 aataccttga atcttatctg cgttgatctg cgttcatctg cgtcccaaat aatcaatcaa
     2101 gcttcttcca ctgcccgccc gagatttgca ccataaccat ggagtcggtt cccaacccgt
     2161 tgtggtcttc ggcggtaagg ttgtaggtcc cgctgatccc cacgtagcct ttggtcgcct
     2221 cgatggcgtc gcgtagcttt tcattgtccg tcccggcctt cttcatggcg ttggcc
//
LOCUS       DASPRR010000570         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1427290, whole genome shotgun sequence.
ACCESSION   DASPRR010000570 DASPRR010000000
VERSION     DASPRR010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427290"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VEL68_20975"
     CDS             <1..397
                     /locus_tag="VEL68_20975"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXZ38515.1"
                     /translation="KPFSPRELVARVKAILRRGRLGREEEGKVLSHEDLVVDLEKHRV
                     TLGGRLIILTPHEFKLLQTLMASPGRVFSRDQLVNHLYPQQEAVVIDRVVDVHIGKLR
                     QKIEKDPTNPRFILTVRGIGYQFADRESG"
     gene            411..1484
                     /locus_tag="VEL68_20980"
     CDS             411..1484
                     /locus_tag="VEL68_20980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ38516.1"
                     /translation="MLTQSLLGKLLGINILIIGFVILIVWVAVDYLAAGYFMTLMEKY
                     KISPTSSHQMFLSAVHRYLIWASLGAVLLAVALSFLLMRRVLGPLRQMTEITRKISAG
                     DYSGKVPMASRDEVGQLAAAFNRMAEGLQRIEQLRKTMVIDVAHELRTPLTNIRGYLE
                     AIGDGVVHPSKETFDLLQEETLRLGRLVEDILNLAKADAARMDLRKINLQVSRTILQG
                     LDTFRPQFSIKEIKVTTDFSEDAVPLPADPEKMGQVVTNLLQNAWQYTPQGETVRIFA
                     RRTSEEIRFTFANPGGGLSQKDLPLIFERFYRGEKSRSREHGGAGIGLAIVKGLIEAH
                     GGRVGAELSDSEVRIWFSLPTTP"
BASE COUNT          538 a          607 c          599 g          505 t
ORIGIN      
        1 gaagcccttc agcccccggg aattagtggc ccgtgtcaag gccattctcc gcagggggcg
       61 tttgggaagg gaagaagagg gaaaggttct ctcccatgaa gacctggtcg tcgatttgga
      121 aaagcataga gtgaccttgg gcgggcgtct cattatcctg acccctcatg aatttaaact
      181 ccttcaaacg ctcatggcct ccccgggaag agtcttcagc cgagaccagc tggtcaacca
      241 tctttatccc cagcaggaag cggtcgtgat cgaccgggtg gtggatgttc atatcggaaa
      301 gctccggcag aaaatcgaaa aggacccgac aaatccccgc ttcatcctca cggtgagggg
      361 gattggatat cagtttgccg accgggaatc aggatgaagg aaagcgacag atgttgaccc
      421 aaagcctgct cgggaagctc ctgggaatca atattttgat catcgggttc gtcatcctta
      481 ttgtgtgggt ggccgtcgac tacctggcgg ccggctattt tatgacccta atggaaaaat
      541 ataaaatctc ccccacttcc agccatcaaa tgtttttgag cgccgttcat cggtatctca
      601 tctgggcgag tttgggggcc gtcctgttgg cggtggcctt gagcttcttg ctgatgcgca
      661 gggtattagg ccccctccgg cagatgaccg agattacccg gaagatctcg gcgggggact
      721 actcgggaaa agtccccatg gcctcccgcg acgaggtggg ccagctggct gcagctttca
      781 accgcatggc cgaaggcctt cagcggatcg agcagctgcg caagaccatg gtgattgatg
      841 tggcccatga gctgcggacc cccctgacca acattcgagg gtatctggag gcgatcggcg
      901 acggggtggt gcatccttcg aaggagacct tcgatctgct tcaggaggaa acgctgcggc
      961 tgggacgctt ggtggaggat attttgaacc tggccaaggc cgatgcggcc aggatggacc
     1021 tccgtaaaat aaacctccag gtttcccgca cgatcctcca gggcctagat actttccgac
     1081 ctcaatttag catcaaagaa attaaagtga cgacggattt ttccgaggat gccgtccccc
     1141 ttccggccga tcctgaaaag atgggtcagg tagtcaccaa cctccttcag aatgcctggc
     1201 agtatactcc gcaaggtgaa accgtcagaa ttttcgccag gcgtacttcg gaagagattc
     1261 gattcacctt tgccaatccc ggcggcgggc tttcccagaa ggaccttccc ctgatcttcg
     1321 aacgcttcta ccggggcgag aagtctaggt cccgagaaca tggaggcgca ggcatcgggc
     1381 tcgccattgt caagggatta atcgaagccc atgggggccg cgtgggggcc gaactttccg
     1441 acagcgaagt ccgtatctgg ttttccctcc caaccactcc ctgatccccc tccgatctct
     1501 acagattctt tcatatcccc tttgattcga tccttacttc tcagattatt attcaagcgg
     1561 agggaaagaa ggtaaaggaa aggattccac tttggggggc atggccaact ctcccaaggg
     1621 atgaaattcg ggcaacttgt cagaagagga cggaggcttt aggcctcaaa gattgaatgg
     1681 aggtaaagag aaatgagaaa cttatggttg attttcacag cggtaatttt cctcttgggg
     1741 tttgccgcct tctctatttt tcgattccct gaaatgtccc cgctcgcttt aagacagctt
     1801 ccatctcctg gaccgtgatc agggcaggat cataataaac tgtgtttatc tccctcccgc
     1861 ccttgaagcc cctttcgacc ttctcgacac ccctcagccc ctccagggct gcttttccaa
     1921 catcgtatca atttacgaaa aagacgacct tagaaagacc ggctgactcg ccatgaacag
     1981 gggaaagggc agtcaggagg atagaaatca aagcgaagat aagaaacaat cccttcattc
     2041 ttccaaccat cagtcgacga gccctcctct tctccgtttt tcaaaaaaag aatgatgaac
     2101 tccagcagac ccgggggtta aaaacagtga accgttcaaa acagatcgaa aagggcacca
     2161 cctaaaggtg gccatggcct ggacaccaat gccaggcccg ggggcaaggg cctggggttc
     2221 aaaaaccatg aggcgtcctt catttcatg
//
LOCUS       DASPRR010000571         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1107382, whole genome shotgun sequence.
ACCESSION   DASPRR010000571 DASPRR010000000
VERSION     DASPRR010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107382"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VEL68_20985"
     CDS             complement(<1..385)
                     /locus_tag="VEL68_20985"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXZ38517.1"
                     /translation="MTEPYRLTLTEMIRQIHRRKLSPVALAESLLKRIDALEPSIQAW
                     VTLDRQMVLEEARRCEKEIARGRKCGPLHGIPLGVKDIFYTAGVKTTGGSKVLADFVP
                     SFDSTAVLRLKQAGAIVLGKTATTEF"
     gene            complement(473..652)
                     /locus_tag="VEL68_20990"
     CDS             complement(473..652)
                     /locus_tag="VEL68_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38518.1"
                     /translation="MPAPTKEETLLMILKDSAIKKYGEERAKVLEASLRDLARALVVI
                     ENYPLEMEEEPSFAR"
     gene            complement(684..1832)
                     /locus_tag="VEL68_20995"
     CDS             complement(684..1832)
                     /locus_tag="VEL68_20995"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ38519.1"
                     /translation="MKALTSRERVILALDHKEPDRVPIDLGSIASTIRTVDAYDRLKE
                     YLGLARDKRIRHFADEHIIPDEEIIQALHVDTVYLRVNVPKSWKRERVDAYTVIDEWG
                     VPWTKAPGSLYTFPGAHPMETAEMAEIEKFPWPDPNEPSRYEGLKEKAQKLFRETDYA
                     LIAEGLTGVGVFDMTWHLRGMENIFMDMILHPEFTEALFRKLTDFYVGVYRNYLKAVG
                     EYIQMVIYYEDLCGQDGPLISPDLYRRYVKPCHRRIFQAIRENTQAKVCVHTCGSVYA
                     FLDDYVELGVEVINPVQTSARDMDPHRLKEKYGKVLSFHGAIDTQRFLPRATPAEVEE
                     EVRRMIRTLGRGGGYLFTSCHSIQPDVSPEKIVALFHSAAKHGKYPLV"
     gene            complement(1965..2471)
                     /locus_tag="VEL68_21000"
     CDS             complement(1965..2471)
                     /locus_tag="VEL68_21000"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXZ38520.1"
                     /translation="MEPEDVLDFICRRRSVRRFQKKTIPEETLTLLLKAAMAAPSGNN
                     AQPWEFVVVRDPKVKQDLSRVHTWVYMVADAPAAIVVMGDQGSEWWAEDCAAATENLL
                     LSAANLGLGTVWCGIKEHDAKTVRKVLGAPERLGVLCIIAVGYPAESPSPHTKYRKEK
                     VHWDRFGK"
     gene            complement(2475..3230)
                     /locus_tag="VEL68_21005"
     CDS             complement(2475..3230)
                     /locus_tag="VEL68_21005"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXZ38521.1"
                     /translation="MAQKNFWSVTDLVRVPPFPPKIVSPVDSAEMVLIPAGEFTQGIT
                     ENELNQVFILDGSQNPVFMTEVPARRVNLEAYYIDIHPVTNYQYEKFIKETGHRTPVL
                     WGKEGWNEPLQPVVGLGWEDARTYAAWAGKGLPTESQWEKAARGKDQRWWPWGNDFYP
                     GYCNCSELGINRTTDVTRFHQGVSPYGCYDMSGNVWEMCEGAWIEGHLPMRGGCFLGS
                     ATFVRTTVRWSAEDQVNGAHWLGFRCVKNLSEG"
BASE COUNT          650 a          976 c          918 g          746 t
ORIGIN      
        1 cgaattccgt ggtcgccgtc tttcccagta cgatggcccc ggcctgcttg agccgcagga
       61 cggcagtgga atcaaaagag gggacaaaat cggctaggac cttcgacccg cccgtggtct
      121 tcacccctgc cgtatagaaa atatctttga cccccagggg aattccatgg agggggccgc
      181 attttcttcc tcgggcgatt tccttttcgc aacgccgggc ttcttccagg accatctgac
      241 ggtccagggt cacccaggcc tggatggaag gctccagggc atcgatgcgt ttcaggagcg
      301 attcagccag ggccaccgga gagagtttcc tccgatgaat ctgccggatc atttcggtta
      361 aggtgagacg gtacggctct gtcatttcca cctccgaccc aacaagcata gggcatagag
      421 catagagaaa gaaagatacg ccatgcgcta tgccctatgc tcttggcctt tttcaccgtg
      481 cgaaagaagg ttcttcctcc atctccagag ggtaattctc gatcacgacg agggcccggg
      541 cgaggtcccg aagcgaagct tctaggacct tggccctttc ctctccgtat ttttttatcg
      601 ccgaatcttt caagatcatg aggagggttt cttctttcgt gggggcaggc attctcatcc
      661 tccttttctc tcaaagcgcc atttcaaacc aggggatatt ttccatgctt cgccgccgaa
      721 tggaagaggg ccacgatttt ttccggggag acgtccggtt ggatgctgtg gcaagaggtg
      781 aatagatatc cgcctcctcg gcccagggtc cgaatcatcc gccggacttc ttcctcgacc
      841 tccgccgggg tagcccgggg aagaaacctc tgggtgtcaa tggctccgtg gaaggaaaga
      901 accttcccgt acttttcttt gaggcgatgc gggtccatgt ccctggcgct ggtctgaacg
      961 gggttgatca cctccacccc caactccaca taatcgtcca ggaaggcgta caccgacccg
     1021 caggtgtgga cgcagacctt ggcctgcgtg ttttccctga tggcctgaaa aatccgccgg
     1081 tggcagggtt tcacgtacct gcggtagagg tcgggggaga tcagggggcc gtcctgcccg
     1141 cagaggtctt catagtaaat gaccatctgg atgtattcgc ccaccgcctt caggtagttg
     1201 cggtagaccc ccacgtagaa atcggtcaat ttccggaaaa gggcttcggt gaattccgga
     1261 tggaggatca tatccatgaa aatattttcc atccccctca ggtgccaggt catgtcgaag
     1321 actccgacgc ccgttagccc ctcggctatc agggcataat ccgtctcccg gaaaagcttc
     1381 tgggccttct ccttcaggcc ctcataacgg ctgggctcgt tggggtccgg ccaggggaac
     1441 ttttcgattt ccgccatttc ggcggtttcc atggggtgag cgccgggaaa cgtatacagg
     1501 gacccgggcg ctttggtcca gggaacgccc cattcatcga tcaccgtata tgcgtccacc
     1561 cgttcccttt tccagctctt ggggacattc acccggagat acactgtatc cacgtgcaat
     1621 gcctggatga tctcctcgtc gggaatgatg tgctcgtcgg caaaatgacg gattcgttta
     1681 tcccgggcca ggcccaggta ttccttcagc cggtcataag catccacggt tcggatcgtt
     1741 gaagcaatgg agcccagatc gatgggcacc cggtcgggct ccttgtggtc gagggccagg
     1801 atgacccgct cccgggaagt gagagccttc attcaattcc tccgttttgc gaaaagactg
     1861 gcgttcgtga gccgtccgtc ctcattcgct tgatggacct tcccacttgc tccgaacccc
     1921 gtaggggcgg gtttcaaacc cgcccctact tggaataatc cccattattt gccgaatcgg
     1981 tcccagtgga ctttttcctt gcggtacttg gtgtgaggag agggagactc ggccggatac
     2041 ccaacggcga tgatacagag gactcccagc ctttccggag cccccaggac cttgcggacg
     2101 gtcttggcgt cgtgctcttt aatcccacac cagaccgttc ccaagccgag gttggccgcc
     2161 gacagcagga ggttttcggt cgctgcagcg cagtcctccg cccaccattc cgagccttgg
     2221 tcccccatca ccacgatggc cgcgggggcg tcggctacca tgtagaccca agtgtgaacg
     2281 cgggagaggt cctgtttgac cttgggatcg cggaccacga caaactccca aggctgggca
     2341 ttgttgccgc tgggggcggc catggccgct ttcaggagca gggttaaggt ctcttcagga
     2401 atcgtctttt tctggaagcg gcggacactc ctccgccgac agataaaatc caggacatct
     2461 tcgggttcca taatctaccc ctcggacaga tttttcacgc aacggaagcc cagccagtgg
     2521 gccccgttca cctggtcttc ggcggaccac cggaccgtag tacgcacgaa agtggcactt
     2581 cccaaaaagc accctccgcg catggggaga tgaccttcga tccaggctcc ctcgcacatc
     2641 tcccaaacat ttcccgacat gtcgtagcaa ccgtaagggc tgactccttg atggaaccgg
     2701 gttacgtccg tagttcggtt gatccccaac tcgctgcagt tgcagtagcc ggggtaaaaa
     2761 tcgtttcccc atggccacca ccgctggtcc ttcccccgag ccgccttttc ccattgagat
     2821 tccgtgggga ggcccttccc cgcccaggcc gcatacgtgc gggcatcctc ccaccccaat
     2881 cccactaccg gctgaagggg ctcattccat ccctctttcc cccacagaac cggggtgcga
     2941 tgtccggttt ccttaatgaa tttctcgtac tgatagttgg taaccggatg gatgtcgatg
     3001 taataagcct ccaggttgac tctgcgggcc ggaacttcgg tcatgaagac gggattctgg
     3061 gaaccgtcga ggatgaagac ctggttgagc tcattctccg tgattccctg ggtgaattcg
     3121 cccgcgggga tcaggaccat ttcggcggag tcgacggggg agacgatctt agggggaaaa
     3181 ggcgggaccc ggaccaaatc cgtgacggac cagaaattct tttgggccac tgaagtcccc
     3241 tccggaattt tttggggcta attatagctt atttgaggaa gagggcaaga
//
LOCUS       DASPRR010000572         4777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_285679, whole genome shotgun sequence.
ACCESSION   DASPRR010000572 DASPRR010000000
VERSION     DASPRR010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285679"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VEL68_21010"
     CDS             <1..993
                     /locus_tag="VEL68_21010"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ38522.1"
                     /translation="IAGVPFAIFSRVVIEPTQDNVEEGVKAYKEAGVDFLIAVGGGSS
                     MDTAKAIGALATNTGKISDFMGANKIPKPAAPLIAVPTTAGTGSEVTPFTIITDTAKD
                     VKMLIASPNILPRVALVDPLMTLTMPQGITAATGLDALTHAIEAYVSIKAQPVTDTLA
                     LQAIRLISANLRQAWSNGDHLEARTNTLIGSLHAGLAFANSSVALVHGMARPIGAYFH
                     VPHGVSNAALLPTVIEFSILGNPKKYADIAEAMGEVTEGLSLLDAAYLAADATRRLND
                     DLKVPTLSGLGVEEKKFNTVVKQMAADAIASGSPGNNPRKATQEEIVELYKKAF"
     gene            1142..1522
                     /locus_tag="VEL68_21015"
     CDS             1142..1522
                     /locus_tag="VEL68_21015"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ38523.1"
                     /translation="MGKYIVNLNEVPVGKIMGRETRDLISGKTVGSKGISLRITDVLP
                     GALCTPGHAHTECEEVIFILSGRGEIKIGEETFPMKVGDAVHLPTGVPHLIRNTGKEV
                     MRMACSFSSDDISRDLKNDETMRF"
     gene            complement(1665..2243)
                     /locus_tag="VEL68_21020"
     CDS             complement(1665..2243)
                     /locus_tag="VEL68_21020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXZ38524.1"
                     /translation="MKEDRKRWDWKYRDREFDPSHGASPILKKYLRFFPRGKALDLAA
                     GEGRNAVFLAEHGFEAEAVDISPVAISRARKSARARGVTIRAFAADLDTYSILRGHYD
                     VILDFYFLDRHLIPEIKKGLKKGGMVVFETYTAEQKKLGTEGPGQDKYLLKPNELLRL
                     FRGFHVLFYREGVFREGGKRRAIASLIAQKNK"
     gene            2569..3129
                     /locus_tag="VEL68_21025"
     CDS             2569..3129
                     /locus_tag="VEL68_21025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXZ38525.1"
                     /translation="MAKKISILVFGGSLRKGSYNKALLRAALEMVPADAQMEAFDIAA
                     IPAFNQDVEKDPPQVVKDFKAKIKAADAILIVTPEYNYSIPGFLKNAIDSASRPYGDN
                     AFDGKPVAFMGASIGMLGTARAQYHLRQACVWLNMYPMNLPEVMVPFAQEKVDESGKL
                     TDGKTREKIKELLQALVIWARRFKKD"
     gene            3213..4454
                     /locus_tag="VEL68_21030"
     CDS             3213..4454
                     /locus_tag="VEL68_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ38526.1"
                     /translation="MDQPRISLYGAHWCPDCRRSKQFLGEHQIPYRWVDIEEDPEAEK
                     YVLRKNSGRRIIPTIEFPDGSILAEPTNAELAAKLGLKTKARRSHYDLLIVGGGPAGL
                     TAALYASREGIEALVIERAAMGGQAATTEYLDNVPGFDQGIEGSLFGQKLRTQAERFG
                     AELLQAQEVVRVSSHDNYHCTDTADGSQYSGCALLLATGSRYRRLGIPGEESYVGSGV
                     HFCATCDGPFYRGKSVAVVGGGNSAAEESLFLVKYVERVTLLVRGEELKGSQVIREKA
                     LNHPRIDVRFHTEVAEFHGEKGKLTGITLRNNQTEARETIHPAAVFVFIGQSPNTAFL
                     KKSRVLLDRWSYILTGHDLLHAQDRPVGYENREPSLLETSIPGIFAAGDARAGSTKQV
                     ASASGEGATAALLIREYLKTT"
BASE COUNT         1124 a         1286 c         1332 g         1035 t
ORIGIN      
        1 attgccgggg ttcccttcgc cattttcagc agggttgtaa tcgagcccac ccaggacaat
       61 gtcgaagaag gtgtgaaggc ctacaaggag gccggggttg acttcttaat cgccgtaggg
      121 ggcggaagct cgatggatac ggccaaagcg attggagctc tggcaaccaa tactgggaaa
      181 atcagcgatt tcatgggggc gaacaagatt cccaaacctg cagcgcccct cattgcggtc
      241 cccacaaccg cgggaacagg cagtgaagtg actcctttca ccatcatcac ggacaccgcg
      301 aaggacgtga agatgctcat cgccagcccc aacattctcc ctcgggtggc cctcgttgat
      361 cccttgatga ctctaaccat gccccagggt atcaccgccg ccaccggcct ggatgccttg
      421 acccacgcca ttgaagccta tgtctcgatc aaagcccagc cggtcacaga tactctggcc
      481 ctccaggcca tccggctcat ttccgccaac ctccggcaag cctggtccaa tggggatcat
      541 ctggaggccc gaactaacac cctgatcggg tcgcttcacg cggggctggc cttcgccaac
      601 tcctccgtgg ccttggttca cgggatggcc cggcccatcg gggcctattt ccatgtgcct
      661 cacggggtct ccaatgcggc cctcctgccc acggtcatcg aattcagcat cctcggcaac
      721 ccgaagaaat atgcggatat tgccgaggcc atgggagaag tcaccgaggg gctttccctc
      781 ctggacgccg cctatctcgc ggctgatgcg acccggcggc tgaatgatga tctgaaagtc
      841 cccaccctca gcgggctcgg cgtggaggag aagaaattca ataccgtggt aaagcagatg
      901 gccgccgacg ccatcgccag cggaagcccg gggaacaacc cccggaaggc cacgcaggaa
      961 gaaattgtgg aactctacaa aaaggccttt taattgggaa ttgcggattg ggaattggaa
     1021 aaacagaatt caggagtcag aagcgggaag agaaaaggaa ctattttgtt cgacgcttct
     1081 tctgaattct gactccgggc tcctgggttg ttgatttatt aaggcggaag gagatcttta
     1141 aatgggcaaa tacatcgtca acctgaatga ggttccggtc gggaaaatca tgggaaggga
     1201 aacgcgcgat ttaatcagcg gaaaaacagt ggggtcaaag gggatctctc tgcgaattac
     1261 agacgtactt ccgggagccc tctgtacacc tgggcacgcg cacacggaat gtgaagaggt
     1321 aatcttcatt ctctccgggc ggggagaaat caagatcgga gaagagactt ttcccatgaa
     1381 agtgggggat gcggttcatc tccccaccgg cgtcccacat ctcatccgga acacggggaa
     1441 agaggtcatg cgcatggctt gttccttttc ctcggatgac atttcgaggg acctgaagaa
     1501 tgatgaaacg atgaggtttt gataagacag gtcatgggta ataggtcaga ggtggtgggt
     1561 catagattat gggtaataga taatagcata tagatcatag gttataaagt ccaaatcatg
     1621 ggtttaatgt tgaatgcccg agtattcgat cgccctccaa cctcttactt atttttttgg
     1681 gctatcaggc tggcaatcgc ccttcgtttg cccccttccc tgaatactcc ctcccggtaa
     1741 aaaagaacat gaaatccccg gaagagcctt aggagctcgt tgggcttcag aaggtatttg
     1801 tcttggccgg gaccttctgt acctagcttt ttttgctctg ccgtataggt ttcgaaaacc
     1861 accattcctc cctttttcag gcccttttta atctcgggaa tcaggtgccg gtcaaggaaa
     1921 tagaaatcga gaataacgtc ataatgcccg cggaggatag aataggtgtc caggtctgcg
     1981 gcaaaagctc ttattgtaac cccgcgggcc ctggctgatt ttcgggcccg gcttatcgca
     2041 accggggaga tatcgacggc ttcagcctca aagccatgtt ccgccaggaa gacggcattc
     2101 ctcccttccc cggcggccag gtcaagggcc ttgccccggg ggaaaaagcg aaggtatttt
     2161 tttaaaatcg ggctggcccc atgactggga tcaaattctc tatccctgta tttccagtcc
     2221 cagcgcttac ggtcttcttt cattttttta tcctggtgca agtgactata ttctaccaat
     2281 cgtcttctct ttttgccatc gggttgggag gtgcgaagtt aggtgcaggc acggagggaa
     2341 accctggagc ccaataccgc atctagattg cgaagagggg taaaaacttt cagaaatggc
     2401 cggaggccgt ctccgattct tatggaatgg tgcaaccccg agggggaaaa tacaattctt
     2461 ttcgccgcaa aatgggggaa tcaccccatt ccgtccctgg ccagagtagc ggaaaataaa
     2521 taattagact aagccgctca acccccttca tccagaaagg agtcagaaat ggccaagaaa
     2581 atttcgattc tggtttttgg aggaagcctg cgaaagggtt catacaacaa ggctttgcta
     2641 cgtgccgcgc tagagatggt ccctgccgat gcccagatgg aggccttcga tatcgcggcg
     2701 atccccgctt tcaatcagga cgtggagaaa gacccgcccc aggtcgtcaa ggatttcaag
     2761 gccaagatca aggccgccga cgccatcctg atcgtgaccc cggaatacaa ttactccatc
     2821 cccggatttc tgaagaacgc catcgattca gcgtcccggc cttacggaga caacgctttc
     2881 gacggaaagc ccgtggcttt catgggggcc tccatcggca tgctgggaac ggcccgcgcc
     2941 cagtaccacc tgcgtcaggc ttgtgtgtgg ttaaacatgt accccatgaa tttgcccgag
     3001 gtcatggtcc cctttgccca ggagaaggtc gacgagagcg gcaagctcac cgacggaaag
     3061 acccgggaaa aaataaagga gcttctccaa gccctggtga tctgggcccg tagattcaaa
     3121 aaggattgaa gattgcaaat cgaaatagtt cactcccgca gtccgagatt ctgaaacctg
     3181 aaccgaattt cttcgaactt ctcgggaggc ttatggacca gccccgcatc tctctttacg
     3241 gtgcccattg gtgtccggat tgccgccgga gcaagcaatt tctgggagag catcagattc
     3301 cgtaccgctg ggtggatatt gaagaggacc cggaggcgga gaaatatgtc ctccggaaga
     3361 atagcggccg gcggatcatc cccaccatcg aattcccaga tggttccatc ctggcggaac
     3421 ccaccaacgc cgagctggca gccaaactcg ggctcaagac gaaggcccgg cgcagccatt
     3481 atgatcttct catcgtcggg ggcgggcccg ccggtttgac ggcggcgctc tatgcctccc
     3541 gggaaggaat cgaagccctg gtcatcgagc gggcggccat gggaggtcag gcggccacga
     3601 ccgaatacct ggataacgtt cccggctttg accaggggat tgaagggagc ctcttcggtc
     3661 agaagctcag gacccaagct gagcgattcg gcgcggaatt gctccaggcc caagaggtcg
     3721 ttcgggtgtc cagtcatgat aattaccact gtaccgacac cgccgacgga agccagtaca
     3781 gcggatgcgc tctcttactg gccacgggaa gccgttaccg gcggctgggt atccccgggg
     3841 aagagagtta cgtgggctcg ggggtccact tttgcgcgac ctgcgatggc cctttttacc
     3901 gggggaagtc ggtggccgtg gtgggagggg gaaacagcgc cgccgaggaa agcctgtttc
     3961 tggtcaaata tgtggagcgg gtgaccctgt tggtccgcgg tgaagagctg aagggttccc
     4021 aggtgattcg cgaaaaggca ttgaatcacc cccggattga cgttcgtttc catactgaag
     4081 tggcggagtt tcatggggaa aaggggaagt tgacggggat aaccctacgc aacaatcaaa
     4141 ccgaggcaag ggaaacgatc catcctgccg cggtgttcgt cttcatcggc cagtccccca
     4201 acacggcatt cttgaaaaag agccgagtcc tgctggaccg gtggagctac atcctcaccg
     4261 gccatgatct tctgcatgcc caagatcggc ctgtcggtta tgaaaaccgt gaaccctccc
     4321 tgctggaaac gagtatccca ggaatctttg ctgcgggaga tgcacgcgca ggaagcacca
     4381 aacaggtcgc ttcggcctcc ggtgaggggg cgaccgccgc tttgctcatc cgtgaatatc
     4441 tgaagaccac ctgaaaggat ctccttcccc caggttctca gcttcggtag gcagcataga
     4501 tgaaatgagc cacgctggcg cattttttct tttaggaaag ctgcagaccc gattctccga
     4561 ttcctgatcg ggctttttcc gccagtttga aaaaatcttt cgggttttca aaagcctccg
     4621 gcgaaaaacg gcttagaatg atgtaagcag ccaacagaag gccccgatgg tcataacggc
     4681 tagtgcggga agagggcgaa attcgtgtct ctcagacttt aggggtttcc taagattctt
     4741 ctacccacta agggcctatt tgaagtattg ataaatt
//
LOCUS       DASPRR010000573        12824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_141951, whole genome shotgun sequence.
ACCESSION   DASPRR010000573 DASPRR010000000
VERSION     DASPRR010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12824)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141951"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            659..1243
                     /locus_tag="VEL68_21035"
     CDS             659..1243
                     /locus_tag="VEL68_21035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXZ38527.1"
                     /translation="MEAAVAVFSQKGYPGGRVADIARQAGISYGQVYTYFKNKEHILL
                     LLFRERWQRFIDYIEKTRKQDIPPTLKLQKVGNFLIRSYEREPDLMKVLTIYVVPNSP
                     FFIKNREAIEQAFRLIRKIFEEGQRTGDFDLSLNPQIAAHGFYGGVLQILLGWIQGII
                     PNSPSNVSKAYQFIQYSSEVSKAARVATQRRPTS"
     gene            1334..2119
                     /locus_tag="VEL68_21040"
     CDS             1334..2119
                     /locus_tag="VEL68_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38528.1"
                     /translation="MANASAPLLLEVNDLHLSFGKVKAIDGVSFGVRRNEILAIIGPN
                     GAGKTCIFNCINGFYQAQRGAIAFEGRPITNLAPHQIAIRGISRVFQHIELFQGLSTV
                     DNLMAARHIHMKRGVFPAALYFGWTRREEIRNRKVVEGIIDFLEIQPIRDRLVGTLPY
                     GMRKRVDLARALAAEPRLLLLDEPMAGMNLEEKEDIARFILDIQEEHGTTIILVEHDI
                     QAIMDIAERVMVIDFGHKVAEGPPEAIVNNPEVIQAYLGKKKA"
     gene            2132..4036
                     /locus_tag="VEL68_21045"
     CDS             2132..4036
                     /locus_tag="VEL68_21045"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ38529.1"
                     /translation="MDAADTFPKLLLRNRGKYGNRRVAMRKKKYGIWNEYTWEACYQN
                     IKTLSLGLIQLGLQRGEKVCICGDNDPEWYWAELAIQSAGGISIGIFIDATPPEIEYI
                     VNHSEAVLVFAKDQEQCDKILQIRQVVPKVRRVVYWDPKGMSAYKDPYLVSLAQVQEL
                     GREREQTQPGLFEENINRGKGSDLAVFCYTSATTGAHPKGAMLTYDYFRSSLDRGAVI
                     DGMTERDEYLSFAPGAWITEQGLGIGWWLREGMKVNFPEEPETVQENIREIGANFLLF
                     GSRQWESLHSAVQMKINDAWVVKRALYNLCMRVGHRTAKYRLNERRHPPFFWVLLNTL
                     CDWVIFRPVRNYLGLLKLRLGITAGTLLGPDIFTWFHAIGVPIKDMYGLTEALYLTSV
                     PPEQVKIGTSGRPLPGVELRISEEGELLLKAENMCGGYYNNPEATRKAFDSEGWFHTG
                     DAAVIDPEGYLIILDRLGDMMVLEDRTRYSPTYLENSLKFSPYIKDAIAIGSGRDFVS
                     AIITIDFENVGKWAEKKRVPYTTFVSLSQRPEVYGLIQKDIQRVNQALPPNLQIKKFA
                     LLHKEFDPDEGEMTRTRKLRRWFLEDKYKDLSSAIYAGEKEIVSEAAVKYRDGRKGRI
                     TTAIRIQDVA"
     gene            4047..4934
                     /locus_tag="VEL68_21050"
     CDS             4047..4934
                     /locus_tag="VEL68_21050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38530.1"
                     /translation="MLYFGQLMITGIMMGGVYGLMAVGVIIIVKASGVFNFAHGAIVV
                     FGAFIFWAFLVQFGLPLWISIPLLFLWAAMLGFLIERLILRPLIGQPLLAAAMATIAL
                     GEILNGIVVLIWPGPGRVYKPPFLPRGAFHLEGLVFPYNQLVCFLACMIALGIFVYFF
                     NYTKQGLAMRGASEDHQLARSVGISVTRVFMISWFIAVLTAAAGGIISASFMGVDPSL
                     GALGLYAFPAVIFGGLESVGGGVVGGISVGILQNLGGGYLDKYVGGGIKEIAPYIIML
                     VVLIFKPHGLFGYKKIERV"
     gene            4968..6002
                     /locus_tag="VEL68_21055"
     CDS             4968..6002
                     /locus_tag="VEL68_21055"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38531.1"
                     /translation="MRYEDTISMCILRTHFHKMLMAIFLLSLYTLPPIFFSDYLLSIL
                     NTTCITIIVVHGLNILTGYCGQINLGQAAFMMAGGYTSAILSTKLGFSFWACLPLAAI
                     SSGVIGFAFGLPALRIKGFYIAMSTLAAQYVLPWLIIHLPTITNGIFGCRANSPTLSG
                     ITFNTEQKWFFLIVSFTILMSFFARNFLRTKIGRAFIAIRDNDVAAEIMGINIFRYKV
                     LAFGLSSIFAGVGGSLMVHYLGVAQYEHFTLLNSIWYLGMLIVGGMGSTTGVIFGVVF
                     IKLLTELTMASTPALVSTFPAIADTVAAAIGPITFGTVIILFLIFEPRGLAHSWATLK
                     ASMRIWPFPY"
     gene            6041..7294
                     /locus_tag="VEL68_21060"
     CDS             6041..7294
                     /locus_tag="VEL68_21060"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38532.1"
                     /translation="MKAKAKENRRGKALGILLVFFFLMGIFLGGPSMAQEPTKVRVFF
                     LGNMTGSYAATETPAFHGWVTTWKWLNKNNYIPGVEVVADWGDAGSDVSKAMSLYKTA
                     MSGATKPVCFQSMNSAQEIALRPWFERDQIPDITPGGAPGTLWPPSYIFSYIAPYVNQ
                     VGAFVDYAKKSWKKDRKPKFGFLTWDNAFGRCIVTDDVINYIKAQGFEYVGAEFFPGA
                     VTEVTTQLLALKNRGADFVYTNTLSAQYAVVLRDFASLGLKMSEMQMGACVWVDPRRT
                     AEIAGEAAEGFLQMQNFEPPSVWSKMPQVMNAFKFGNHPESEFTSEWMSCWIQAHITA
                     EAIKIAAKEVGAKNITGKAVYDALGKVNTDCEGVAKIQYSKDIHWAQTLKIFRVTTKK
                     VGKTKFDSVYAEAATDWFTPPMLMK"
     gene            7493..8284
                     /locus_tag="VEL68_21065"
     CDS             7493..8284
                     /locus_tag="VEL68_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38533.1"
                     /translation="MLKMNNVEVVYSTVILVLRGVSLEVPVSGIVCLLGANGAGKSTT
                     LKAISGLLHTELGEVTHGDITFQERVIDKEPPESIAAMGITQVMEGRRLFPHLTTEEN
                     LLIGGHLTPDRFQLRKRLERVYGYFPKLKELRSQTSGYLSGGEQQMLVTGRALMGEPK
                     VMLLDEPSLGLAPLLVEEIFKILQAINQAEKVAILLVEQNAAIALEFANYGYIMETGR
                     VVLDGPTEKLRENEDIKEFYLGLSQVGQRRGYREVKHYRRRKRWL"
     gene            8397..8879
                     /locus_tag="VEL68_21070"
     CDS             8397..8879
                     /locus_tag="VEL68_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ38534.1"
                     /translation="MKDEWRKKTKPLTLEGGIHMPYSWTVGRVGSRFFIELRDHGRIL
                     GNRCPQCQVVWVPPRLRCPECYVEIDDRSWTEVGPQGTLRHFTIVRYEHPAQPLKPPF
                     AYGLIHLDGATRAITHLISGADMESLKPGIRVKPVMKPVRQGNILDISFFTPVGGPNP
                     "
     gene            8876..10018
                     /locus_tag="VEL68_21075"
     CDS             8876..10018
                     /locus_tag="VEL68_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ38535.1"
                     /translation="MRRVAVVGVSQTCFLPNHREKNYADLVYEVTTSALADAGMEISQ
                     IDNVVTLSNDFWDGRTISSMAVMDACGSYGKNVSTVEGDGNFGALYGAMRIWSGSFGT
                     TLVVAHSKGSEGDPFLITNAMFDPFYHRPLGLDAISSAALQAQTYIDRRGARPEDWAQ
                     VSVKNHRNAKGNPFAQLPMDLTVEQVMASPLKATPLKRLDCSPVSDGAAAVILAAEDR
                     VGELRQKPVWLKGVGYAAELHYLGDRDLSHPRALELAAQKAYAMAGIKDPKGEIDFLE
                     VYDAFSYMEPLWLEGLGLCAPGEGGKWTREGITEMKGRLPVNASGGVLSAHAVLVAGL
                     VRFIEVALQIRGTAGAHQLDRARVGLAHGINGPCGQSHCVWLLGNR"
     gene            10096..11250
                     /locus_tag="VEL68_21080"
     CDS             10096..11250
                     /locus_tag="VEL68_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ38536.1"
                     /translation="MGKRVAVTGFGQTHHSARRWDVNGLELIQEAVTRALADAQLNLK
                     DIDAVVIGNMDHFEGINYVDTWSVEGSGTFLKPIFKVTTGGTTGSTVGIAAYYHVASG
                     LFDHVLAIGWEKNSESDTTGAIITCSDAIWDRFSYSGAIPSLAVEASAYMKRYGITQQ
                     DAARVTVRDRGNAVNNPYAHLRTAVTVEDVMHSPMLTEPIKLLDICPRSDGAAAVIFS
                     SEDKAGKTCSRPAWVHSVSVRHDTTWFGDIDYVGIPSLASAAKEAFHLAGIREPWKEL
                     DVCELYMPYSFAGLKWIESIGFCPPGQAPRLLWDGETDMGGNLPINPSGGVLSTNCIG
                     ATALLRVGEVAQQIMGKSDKRQVPDVRLGMATGFGGCFWSDVMILGADKP"
     gene            11332..11823
                     /locus_tag="VEL68_21085"
     CDS             11332..11823
                     /locus_tag="VEL68_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ38537.1"
                     /translation="METRASERNPSLLAMEEIAHMPYHWAIGLDGSKFFQEIKEREVL
                     LGVRCPKCRKVYVPPRLVCGPCFVKMDELVELGKEGVVEAVTLVNYPFIDPDTGDRRP
                     VPYIYGYIKLGGADNLFSHIIKTPPGISVQVGDRVRAVFSREKKGRIQDIAFFEPLPK
                     RKP"
     gene            11869..>12824
                     /locus_tag="VEL68_21090"
     CDS             11869..>12824
                     /locus_tag="VEL68_21090"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ38538.1"
                     /translation="MEFTKEQILLRETVARFAREQVAPLAAEIDQKERFPRETFSKMS
                     ALGLLGLGIPEEYGGSGGGIVESCIVAEELARYCVSTSASWGAHVDLCAANICRNGTT
                     EQKQRFLPDLASGRKLGGMAMTEPEAGSDVLAMKTRAVEKGDFFILNGSKTFITNGPI
                     GDLFLVYATTDPKSRGKGVTAFVVEKGFPGFSCGKEFEKLGWHGSPTGELIFEDCPVP
                     RENLIGTVNQGVKVLLSGLNSERLVISANCLGLARASLEDSVKYSRERKQFGKAISEF
                     QMIGEKLARMVAKVEAVRCMLWSQAFRMSREGPAAMAFETAC"
BASE COUNT         2849 a         3402 c         3754 g         2819 t
ORIGIN      
        1 caggattcat aactatcgcg gtcttatgga aaagctcaga atgaacagcc cgtacaaaat
       61 ggataaagaa gtgccggatc aagcgcctta cccaaaataa atgccactgt tccttccgat
      121 cagtatctga ttcccaatcc aaatagaagt gaataaaaac ctctgagttt tttaagacca
      181 acctttagcg actcttccgg ttagatacga ttcccatttt ttcagcccat gaaatccctt
      241 ccgacaataa atatttctcc ggtgaattga tgaagattat gaaaagagct gggtaaagaa
      301 gaagtagtgg gaaatagggt gttggtttat gtggtcttcg taaacatccc ccttccactc
      361 gaagtgaacc cgtcagggct gaccgccagt cccgtggtat gggccacgag accgttgttg
      421 aagttgttac ccgtcccatg caagtctttg ggtgagcttc ctaggcaaat tttgaatttt
      481 gaatttttct cttgacaaaa aaattggcaa atgatagacg ctgattgata tgtcaatcaa
      541 tttctaatgc ggggtggaat tgacacggct aaaccgtaag gctttttccc ggaatcgttt
      601 ccctcgggag cggactgggg ggagaagtgc tcgaaatacg gaaaaaagag ctctgatcat
      661 ggaagcggcg gttgctgttt tttctcaaaa gggatacccg ggaggtcgag tcgcggacat
      721 tgccaggcaa gccggaattt cttatggcca ggtttatacc tatttcaaga acaaagagca
      781 tatcctcctg ctgctctttc gggaacgctg gcagcggttc attgattata tcgaaaaaac
      841 caggaaacag gacatccccc ccaccctgaa attgcagaag gtggggaatt ttctgatccg
      901 cagttatgaa cgcgaacctg acctcatgaa agtcctcacc atttacgttg tccccaattc
      961 tccttttttc ataaagaatc gggaggcgat agagcaggcc ttccggctga ttcggaaaat
     1021 atttgaagaa ggacagcgga caggggattt tgatctgtcc ctgaacccac agatcgccgc
     1081 tcatggattc tacggcggcg ttctccaaat cttattggga tggatccagg ggattattcc
     1141 caattccccc tcgaatgtat ccaaggccta ccagttcatt caatattcga gcgaggtctc
     1201 gaaggcagca agagtagcga cccaaagacg ccctacttcc taacggggat cctgcgattt
     1261 cccttgcgat cgcggggatc gacggttcta ggccccttct ctgccggctt tgtctgagcg
     1321 gaggtttcag gaaatggcga atgccagtgc cccccttctc ctcgaagtca acgacttgca
     1381 tctcagtttc ggaaaagtga aagcgatcga tggggttagc tttggggtca ggagaaatga
     1441 aatcctggcc atcatcggcc ccaacggagc ggggaaaacg tgcatcttta actgcatcaa
     1501 cggcttctat caggctcaac ggggggcgat tgccttcgaa gggcgtccca tcaccaacct
     1561 ggcgccgcac caaatcgcca tccgggggat ctcccgcgtc ttccagcata tcgaactgtt
     1621 ccaaggcctg agcacggtgg ataatctcat ggctgcccgc catatccaca tgaagcgggg
     1681 ggtctttccg gcggccctct atttcggctg gacccgtagg gaagagattc gaaaccggaa
     1741 agtggtggag gggattatcg acttcctgga gatccagccc atccgggaca ggctggtagg
     1801 aacccttccc tacgggatgc ggaaacgggt cgatctggcc agggccctgg ccgcagagcc
     1861 tcggctgttg ctcctggacg agcccatggc cgggatgaat ctggaggaga aagaggacat
     1921 cgcccgtttc atccttgata tccaggaaga gcacggaacc acgatcatcc tggtcgagca
     1981 cgacatccag gccatcatgg acatcgccga acgggttatg gtcatcgatt tcgggcataa
     2041 ggtggccgag gggcccccgg aagcgatcgt gaacaacccc gaggtcatcc aggcctacct
     2101 gggcaaaaaa aaggcataag ggccaaagag aatggacgcc gccgatacct tccccaaact
     2161 tctgctccgc aatcggggaa aatacgggaa ccgccgcgtg gcgatgcgca agaaaaaata
     2221 cggcatctgg aatgagtaca cctgggaggc ctgttatcag aatatcaaaa ccctctccct
     2281 ggggctcatc cagctgggtc tgcagcgggg cgaaaaggtc tgcatctgcg gagacaacga
     2341 cccggaatgg tattgggcgg aactggccat ccaatcggcg gggggcatct ccatcggcat
     2401 cttcatcgac gccactcccc cggaaatcga gtacattgtc aatcacagcg aagcggtcct
     2461 ggtcttcgcc aaggaccagg agcagtgcga taaaattctc cagatccggc aggtggtgcc
     2521 caaggtccgc agagtggttt actgggaccc caaagggatg tccgcttaca aggaccccta
     2581 cctggtcagc ctggcccagg tccaggagtt gggacgggag cgggagcaaa cccagccagg
     2641 cctgttcgag gagaatatca accgggggaa gggaagcgac ttggcggttt tctgctacac
     2701 ctcggccaca acgggagctc atcccaaagg ggccatgctc acctacgatt atttccgctc
     2761 ttccctggat cgcggggcgg tcatcgacgg gatgaccgaa agggatgaat acctctcctt
     2821 cgctcccggg gcctggatca cggagcaggg attggggatc ggctggtggc tccgggaagg
     2881 aatgaaggtg aattttcccg aggagccgga gaccgtgcag gagaatatcc gggagatcgg
     2941 agcgaacttc ctgctttttg ggtcccggca gtgggagagc ctgcactccg cggtgcagat
     3001 gaagatcaac gacgcttggg tggtgaagag agccttgtac aacctttgca tgcgggtggg
     3061 ccaccggacc gcgaagtacc gtctgaacga gcgccggcat cctccgtttt tctgggttct
     3121 tctcaatacc ctttgcgact gggtcatttt ccgcccggtc cgcaattatc tgggcctttt
     3181 aaaactgagg ctggggatca cagccggaac gctcctgggc ccggatatct tcacctggtt
     3241 ccatgccatc ggggtgccca tcaaagatat gtacggcctt accgaagcgc tttacctgac
     3301 ttccgttccc ccggagcagg tgaagatcgg cacctcgggc cgccctttgc cgggagtgga
     3361 gctccggatc tccgaagaag gtgaactgtt attaaaagcc gaaaacatgt gcggggggta
     3421 ctacaacaac cccgaagcca cccgtaaagc cttcgacagc gaaggatggt tccataccgg
     3481 cgatgccgcg gtcatcgacc ccgaagggta cctcatcatc ctcgaccgcc tgggggacat
     3541 gatggttcta gaggaccgca cccggtattc ccccacgtac ctggagaaca gtctcaagtt
     3601 cagcccgtac atcaaggatg ccatcgccat cggcagcggg agggatttcg tgtcggccat
     3661 cattaccatc gattttgaaa atgtgggcaa gtgggccgag aagaaaaggg ttccctacac
     3721 cacctttgta agcctttcgc aaaggcccga agtgtacggt ctgatccaga aagacataca
     3781 aagggtcaat caggccctac ccccgaactt gcagatcaaa aagttcgccc tgctccataa
     3841 ggagttcgat ccggatgaag gggagatgac ccgcactcgg aaattacgcc ggtggttcct
     3901 ggaggacaaa tataaggatt tgtccagtgc catctacgct ggggaaaagg aaatcgtctc
     3961 cgaagcggcg gtcaaatacc gggacggccg caagggaagg atcaccaccg ccatccgtat
     4021 ccaggatgta gcctgagggg gaggagatgt tgtacttcgg ccagttgatg attaccggga
     4081 ttatgatggg tggggtttac gggctgatgg cggtgggggt catcattatc gtcaaggcct
     4141 ccggagtctt caacttcgcc catggggcca tcgtcgtctt tggggctttc atcttctggg
     4201 cctttctcgt ccagttcggc cttcccctgt ggatcagtat tcccctctta ttcctctggg
     4261 cggccatgct aggattcttg atcgagcgcc tgatcctccg tcccctgatc gggcagccct
     4321 tgctggccgc ggccatggct acgatcgccc tgggggagat cctgaacggc atcgtcgtcc
     4381 tcatctggcc cggccccggt cgggtataca aacccccctt cctcccccga ggggccttcc
     4441 atctggaagg tcttgttttt ccttacaatc aactcgtctg ttttcttgcc tgtatgatcg
     4501 cgctgggcat tttcgtctac ttcttcaatt atacgaagca gggcctggcc atgcgcggag
     4561 ccagtgagga tcatcaacta gcccgttcgg tcgggatcag cgtcacccgg gtcttcatga
     4621 tctcctggtt catcgccgtc ctgacggcag ccgccggagg gattatctca gcttctttta
     4681 tgggcgtcga cccctcgctg ggagccctgg gcctgtacgc gttcccggcg gtcatcttcg
     4741 ggggcctgga atcggtgggc ggtggggtgg tcggcggaat ctccgtggga atcttgcaga
     4801 acctgggcgg gggttatttg gacaaatacg tggggggagg gatcaaagag atcgctccct
     4861 atatcatcat gctagtggtc ctcatcttta aaccccatgg ccttttcggc tataaaaaga
     4921 tcgagcgggt ctgaaccatg cacaatcgcc cttgtggaac ttttgatgtg cggtatgaag
     4981 ataccatcag tatgtgtatc ctgcgcaccc attttcacaa gatgctcatg gccatttttc
     5041 tcctatcgct gtacaccctt ccgccgatct tcttttccga ttacctgttg agtatcctga
     5101 acacgacctg tatcactatc atcgtggttc acggattgaa tatcctgact ggatactgcg
     5161 ggcagatcaa cttgggccaa gccgccttta tgatggccgg cggctatacc agcgccatcc
     5221 tgtcaacgaa gctgggattt tctttctggg cctgcctccc cctggcggcc atctcctccg
     5281 gggtgatcgg gtttgccttc gggctgcctg ccctcagaat caagggtttt tatatcgcca
     5341 tgtcaacgct ggccgcccag tatgtacttc cgtggctcat catccatctg ccgacgatca
     5401 ccaacgggat cttcgggtgc agggccaact ctccgaccct gagcgggatt actttcaaca
     5461 cggagcaaaa atggttcttt ctaattgtgt ctttcaccat cctcatgagt ttctttgcca
     5521 ggaattttct gcgcaccaag atcggaaggg catttatcgc catccgcgac aacgacgtag
     5581 ccgccgagat catgggcatc aacatctttc gctataaggt tctggcgttt ggactttctt
     5641 cgatctttgc cggtgtgggg ggctcactca tggttcatta cctgggggtg gcccagtatg
     5701 aacacttcac cctgctaaat tcgatctggt acttagggat gctgatcgtg ggcgggatgg
     5761 gtagtactac cggggtcatc tttggcgtcg tctttataaa actcctgacc gagctcacca
     5821 tggccagtac cccggctctg gtcagtacat ttccggcgat cgccgatacg gtagcggccg
     5881 ccatcggtcc tatcaccttc ggaacggtga ttatcctctt cctgatcttc gaaccccggg
     5941 gactggccca cagctgggca acgctcaaag cttccatgcg catatggccc ttcccttact
     6001 aaaaaagggg ggaggagaaa cagaaaggag ggaaaaacgg atgaaggcga aagcaaagga
     6061 gaacagaaga ggaaaagccc taggcatcct gctcgtattc ttctttttga tgggtatctt
     6121 tttggggggt ccttccatgg cccaggagcc caccaaagtg cgggtttttt tcctaggaaa
     6181 catgaccggg tcttacgccg ccacagagac gccggctttc cacggctggg tgaccacctg
     6241 gaagtggctg aataagaata attacatccc gggcgtggag gtcgttgccg actggggcga
     6301 tgcgggctcc gacgtctcca aagcgatgag cctttataaa acggccatgt ccggggctac
     6361 caagcctgtc tgcttccaat cgatgaactc ggcccaggag atcgctctgc gcccctggtt
     6421 cgaaagagat cagattcccg acatcactcc gggcggagct cctggaacgc tctggccgcc
     6481 gagctacatt ttcagctaca ttgcgcccta tgtgaaccag gtcggggctt ttgtggatta
     6541 cgccaaaaaa agctggaaga aggaccggaa accgaaattt ggcttcctca cctgggataa
     6601 cgcctttggg cgctgcattg tcactgacga tgtgatcaac tacatcaagg cccaggggtt
     6661 tgaatacgtg ggggcagaat ttttcccagg ggcggtaacc gaggtgacca cccagctcct
     6721 cgccctgaaa aaccggggag cggatttcgt ttacaccaat accctatcag cccagtatgc
     6781 cgttgtcctg cgagattttg ccagcctggg actaaagatg agcgagatgc agatgggcgc
     6841 ttgtgtgtgg gtggaccccc gccggaccgc cgaaattgcc ggagaggctg cggaaggatt
     6901 tctccagatg cagaacttcg agcccccttc ggtatggtcg aaaatgcccc aggtcatgaa
     6961 tgccttcaag ttcggcaatc atccagaatc ggaatttact tcggagtgga tgagctgctg
     7021 gatccaggcc cacattaccg ccgaggcgat caagattgcc gctaaagagg tgggagctaa
     7081 gaacatcacc gggaaggcgg tctatgacgc cttgggaaag gtaaacaccg attgcgaggg
     7141 cgtggccaag atccagtatt ccaaggatat acactgggcc cagaccctga agatcttccg
     7201 ggtcaccacc aagaaggtgg gaaagacgaa attcgattcg gtctatgcgg aggcggcaac
     7261 cgattggttt acccctccca tgctgatgaa atagtgggaa ttgaaaaatc gggagacgtt
     7321 ccggggcgtg ggccatcccg gaaatttcct tgaccggatt tcccttccct ttcgccccct
     7381 cactatcctc ccccccgagg gggagggtaa gggtgggggg tggcgaaaga catctttccc
     7441 tgaacctcgc caggcctata gataacgggc tgaagcagat ggaggtgggc gggtgctcaa
     7501 aatgaataat gtggaggtcg tgtacagcac ggtcatcctg gttctccggg gagtctccct
     7561 ggaggtcccc gtcagcggca ttgtctgcct gctgggcgcc aatggcgccg ggaagagcac
     7621 caccctgaag gccatctccg ggctgctgca taccgagctg ggagaagtca cccatgggga
     7681 tatcaccttt caggagcggg tgatcgacaa ggaacctccc gaaagcatcg ctgccatggg
     7741 catcacccag gtcatggaag ggcgaagact tttccctcat ctgaccacgg aagagaatct
     7801 attgatcggt gggcatctaa cgccggatcg tttccaattg cgcaagcgcc tggagcgggt
     7861 ctacggatac tttccaaaat tgaaagagct gaggtcccag acgagtggct atctatccgg
     7921 cggggaacag cagatgctgg tcacgggaag ggcgctcatg ggtgaaccga aggtgatgct
     7981 tcttgacgag ccttccctgg ggcttgctcc cttgctggtc gaggagatct tcaaaatcct
     8041 ccaggccatc aatcaggcgg aaaaggtggc gatccttctg gtagagcaga atgcggccat
     8101 cgccctggaa tttgccaact acggatatat catggaaacg ggccgggttg ttctcgacgg
     8161 tccgacggag aagctcaggg agaatgagga catcaaggag ttctacctgg gtctcagcca
     8221 ggtggggcaa aggaggggct accgagaagt aaagcactac cgaagaagga agagatggct
     8281 ttaaatacgc cgttggataa aatcgtgcgg ggcccggagt aaaggccgtc ttgccagcta
     8341 tcgtcgaatt cccgaatcgt gaaccggaat cacggaaaaa aatagaggag aaggccatga
     8401 aggatgagtg gcgtaagaaa accaagccgc tgactttgga gggcgggatt cacatgccct
     8461 attcctggac ggtaggccgg gtgggaagcc gattctttat cgagctgcgg gatcacgggc
     8521 gtatcctggg caatcggtgt ccgcaatgcc aagtggtatg ggttcccccg cggttgcgtt
     8581 gcccggagtg ttacgttgag attgacgacc ggtcctggac cgaggtgggg cctcagggca
     8641 cgttgcggca cttcaccatc gttcgatacg aacatccagc tcagcccctg aagccgcctt
     8701 ttgcttacgg cttaatacat ctggatggag ccacccgggc gatcactcat cttatttcgg
     8761 gggcggacat ggaaagttta aagcccggca tccgggttaa accggtgatg aagccggtgc
     8821 gacagggtaa tatcctggat atttctttct ttaccccagt gggaggaccc aatccatgag
     8881 acgggtagcc gtggtggggg tttcgcagac atgttttctg cccaaccatc gagaaaaaaa
     8941 ttatgccgac ctggtctatg aagtgaccac cagcgctctg gccgatgccg ggatggaaat
     9001 cagccagatc gacaacgtgg tcacgttatc caatgacttt tgggacggac gcacgatctc
     9061 cagtatggcc gtgatggacg cctgcggttc gtacgggaag aacgtttcca ccgtggaggg
     9121 ggacggaaat ttcggagccc tttatggcgc gatgcggatc tggtccggct ctttcggaac
     9181 cacgctggtg gtggcccact cgaaagggtc ggagggggat ccttttttga tcaccaacgc
     9241 catgtttgat cctttttacc atcgtccctt gggattggat gccatcagct ctgcggccct
     9301 gcaggcccag acttatatcg atcggcgggg agccaggccg gaagattggg cgcaggtatc
     9361 ggtgaaaaat caccggaatg ccaaagggaa tccctttgcc cagctgccca tggatttgac
     9421 cgtggagcag gtcatggctt cccccctcaa agccaccccg ctgaaaaggc tcgactgctc
     9481 tccagtttca gacggggctg ctgcggtgat cctggcggca gaagaccggg taggcgaact
     9541 ccgccaaaag ccggtctggc tgaaaggggt aggatacgcg gccgagttgc actacctcgg
     9601 ggaccgggat ttatcccatc cacgggccct ggagctggcc gcccaaaagg cttatgccat
     9661 ggccgggatc aaggatccca aaggagaaat cgatttcctg gaagtttacg atgccttctc
     9721 gtatatggag ccgctctggc tggagggctt gggcctttgc gctccgggcg aaggtggaaa
     9781 atggacccgg gaggggatca ccgaaatgaa ggggagactc cccgtgaatg cctccggtgg
     9841 ggttctttcc gctcatgcgg tgttggttgc gggcctggtt cgtttcatag aagtggcgct
     9901 gcagattcga ggaaccgcag gagcgcatca gcttgaccgt gcgcgagtgg gcctagccca
     9961 tgggatcaac ggcccttgcg gccagtccca ttgcgtgtgg ttactgggaa atcgttagtt
    10021 gagagctggg agtcgagagg gggagaatag cccaatttca agagtccgat tttagagaaa
    10081 cgagagggca agcctatggg caagagagtg gcggttaccg gatttgggca gacccatcat
    10141 tctgccaggc gatgggatgt caacgggctg gagctcattc aggaggcggt cacccgggcg
    10201 ctggcggatg cccaattgaa tctcaaggat atcgatgcgg tggtcatcgg taacatggat
    10261 catttcgagg ggatcaatta tgtcgatacc tggagcgtcg aaggttccgg cactttttta
    10321 aagcccatct tcaaggtgac gaccggcgga accaccggca gtacggtggg gattgccgcc
    10381 tattaccatg tggcttccgg cctctttgac cacgtcctgg ccattgggtg ggagaaaaac
    10441 tcggaatccg atacgaccgg agcgatcatc acctgctccg acgccatctg ggaccgtttc
    10501 tcctattcgg gagccatccc gagcctggca gtagaggctt cggcgtatat gaagaggtat
    10561 ggaatcaccc agcaggatgc cgcccgggtg accgtaaggg atagggggaa tgcggtgaac
    10621 aatccctatg ctcacctgag aacggcagtg acggttgagg atgtcatgca ttcccccatg
    10681 ctgacggagc cgatcaagct gctggacatc tgcccccgtt ccgacggggc ggcagcggtc
    10741 atattcagct cggaggataa ggccgggaag acctgttccc gacccgcctg ggttcattcg
    10801 gtttcggtcc gccatgacac cacgtggttc ggggacattg attacgtggg gataccttcc
    10861 ctggcatctg ccgccaagga agcattccat ctggccggga tccgggaacc ctggaaggaa
    10921 ttggacgtct gtgagctcta tatgccttac tcctttgccg gactgaaatg gatcgagtcg
    10981 atcggttttt gcccccccgg acaggcaccg cgccttcttt gggacggaga gaccgatatg
    11041 ggagggaacc tacccataaa cccgtcaggg ggggttctat cgaccaactg catcggggca
    11101 actgctctct tacgggttgg ggaagtagcc caacaaatca tgggaaaatc ggataagcgg
    11161 caggttccgg acgtccgcct gggaatggcc accggattcg gtggatgttt ctggtccgat
    11221 gtgatgattt tgggcgctga taagccatag agcggcaagg cgcagtcctt attccggggg
    11281 attcttttta aaaattctaa gcaactcagt ttgagaacta tagggggaat catggagact
    11341 cgagcttcgg aacgaaaccc ttcactcttg gccatggagg aaatcgccca tatgccatac
    11401 cactgggcga tcgggctgga cgggagcaaa tttttccagg agatcaagga aagggaggtc
    11461 ctgttggggg tccgttgtcc gaaatgccgc aaagtgtacg ttcctccccg gctggtctgc
    11521 ggcccctgtt ttgttaaaat ggatgaattg gtggagttgg ggaaagaggg ggtggttgaa
    11581 gcagtaaccc tggtgaacta tcccttcatc gacccggaca cgggagatcg aaggcctgtt
    11641 ccttacatct acggctacat caagcttggc ggcgccgaca atctcttttc ccatattatc
    11701 aaaacccctc ccggaatttc ggttcaggtg ggggataggg tccgtgcggt tttttcccgg
    11761 gagaagaaag ggcggataca ggacatcgct ttttttgaac ccctgccgaa acgcaaacct
    11821 tgatcccgcg cttcccggga ttgaatcaaa accgctaatc ggagaaacat ggaattcacc
    11881 aaggaacaaa tcctgctccg cgaaacggtg gcccgttttg cccgggaaca ggtcgctcct
    11941 ctggccgcgg aaatagacca aaaggagcgt ttcccccggg aaactttctc caagatgtcc
    12001 gccctggggc ttctgggcct gggcattcct gaggagtacg gcggctcggg cggcgggatt
    12061 gtggagtctt gcatcgttgc cgaagagctg gcccggtatt gtgtctcgac ctccgcaagc
    12121 tggggggccc atgtggacct ctgcgccgcg aatatctgcc ggaacggcac taccgagcag
    12181 aagcagagat ttttgcccga cctggccagc ggccgaaagc tgggcggcat ggccatgacc
    12241 gaacctgaag ccggctctga tgtcctggcc atgaaaaccc gggcggtcga gaaaggggat
    12301 ttctttattc tcaacgggag caagaccttc atcaccaatg gaccgatcgg agaccttttt
    12361 ctcgtatatg caaccaccga ccccaaatca agaggtaagg gggttaccgc ctttgtggtg
    12421 gagaaggggt ttccgggttt ctcctgcggc aaggagtttg agaaactcgg ctggcacggc
    12481 tctcccaccg gagagttaat cttcgaagac tgcccggttc cccgcgagaa cctcatcgga
    12541 accgtcaacc agggggttaa agttctcctc tcggggttga actccgagag gctggtgatc
    12601 tcggccaatt gcctgggtct ggcccgggcc agcctggaag attcggtgaa atattcccgg
    12661 gaaagaaagc aatttggaaa agccatttcc gagtttcaga tgatcggcga aaaactggcc
    12721 cggatggtcg ccaaagtgga agcggtgcgg tgcatgctct ggtcccaggc ttttcggatg
    12781 tcccgggaag ggcctgcggc catggccttc gagaccgcct gcat
//
LOCUS       DASPRR010000574        12704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2237471, whole genome shotgun sequence.
ACCESSION   DASPRR010000574 DASPRR010000000
VERSION     DASPRR010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12704)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237471"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VEL68_21095"
     CDS             <1..1085
                     /locus_tag="VEL68_21095"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ38539.1"
                     /translation="EGRRAILKIHSRRMPLSPDVNLDRLAQITHAFVGADLEALCKEA
                     GMVALRRYLAMEGENDLASKLANSEQLQIGMEDFLLALREIEPTATREIYTERSAVRW
                     HHVGGLRKVKETLLSIVEWPARYPDLYSAGRVLPPRGILFSGPSGTGKTLMAKALAGE
                     TGLNFISISGPILFSKWLGESERALHHIFKKAKQSAPCILFFDEIEALGMTRGTGSDS
                     GAVERMASQFFHEMDNLSDLSQVIVLGATNRKDLLDPALTRAGRLEFVLPFPLPDESE
                     RLEIFQIHTAERPLAPGVDLSDLARRTEGMSGAQIAHTCRTAALAAITEIVKGPEEAR
                     SQKLSLRASHFHGAIEEWGKKESPPC"
     gene            1079..1783
                     /locus_tag="VEL68_21100"
     CDS             1079..1783
                     /locus_tag="VEL68_21100"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXZ38540.1"
                     /translation="MLREVRKKKAHEDIVKQIRSLVEKKMLKQGEKLPPERDLSETFK
                     VSRATVREAIFSLETMKLVRRRQGDGTYITAARGEDLVQPLALSLVHEKDDLIDIFSL
                     RKLIEPEVARLASDNAHPEDIQHLERILREQEREVNRKRSALRIDSEFHQALAQTSRN
                     QIFERLLMAVMDLLQKTREQYFQTEERKRISLMGHRDILGAIRARDGRKARLAMRRHL
                     ERVERIAFKRGGEEKS"
     gene            1872..2510
                     /locus_tag="VEL68_21105"
     CDS             1872..2510
                     /locus_tag="VEL68_21105"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ38541.1"
                     /translation="MAKVTEITVWTRGVVMDKEARDVANAFAQAAKTEGKFIQSFDNY
                     EDLPDRVLVTTRKYARISDEEIAMKYEYANHHPDVVVIVEPTIVKGINVLKGMPEGGL
                     LVINTSRPIDYMLKFIPNADVLGAIATVDADGISGVRTVDFSGSEGGVDAAGIGSGIA
                     APILGAMARVTNMVKKESLTKIVKDVASLERGFKEVKLQKFRKSRAEYFWGS"
     gene            2554..2883
                     /locus_tag="VEL68_21110"
     CDS             2554..2883
                     /locus_tag="VEL68_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXZ38542.1"
                     /translation="MPIKVPKDREVPFGAITPAPVKQQELFITSNWRIVRPVIDHEKC
                     TRCFTCYASCPDACWSFNEKEEKMEWNWKFCKGCQICIYECPADAISAAPELDFEDGV
                     VRLEKPF"
     gene            2895..4085
                     /locus_tag="VEL68_21115"
     CDS             2895..4085
                     /locus_tag="VEL68_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HXZ38543.1"
                     /translation="MMAQERMLSGCAAAAQGAKLAEVEVICSFPIRPYTAIMMELARM
                     VANGELDAEFIHGEGEHAQVSVVLGASAAGARAYTGSSGVGVTYAFEVYSPAAGGRYP
                     LQMAIADRTLDPPGDFGSEHTDAMSARDQGWLMGWASTPQEVLDNTLINFRVGEDPEI
                     MLPQFVCQDGYFLSHIPDKVVLPEMSQVREFLPPYKAPHPLSTECPVSHGPQIRPDQG
                     APMDAQRAATFLEVPRVIEKAVDDFNRIFKRNYDPYLEKYKMEDAEVAYFIQGAHANT
                     CKAAINQLRSKGLKAGMVRPRWVRPWPTQQIAEAFSKVKAIASVETSTSYGGAMRGGN
                     LVHELRASLYDLEKRPLVTSFMAGLGGDVILLEDFDYMAKILTQMIQEKKTRQHVYWI
                     GFEE"
     gene            4117..5106
                     /locus_tag="VEL68_21120"
     CDS             4117..5106
                     /locus_tag="VEL68_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ38544.1"
                     /translation="MAEGFDYKKQILEPIKSIRSAPAEEMYLPGHRTCAGCGPALNYR
                     LTAKAAGKDTIFIGPTGCMYVANSSYLCTPYAVSWIHAQITNGGAVASGIEAAYKIMA
                     RKKKYTGAFPNVIVMAGDGGSVDIGLQAMSAMMYRAHDILFICYDNESYANTGIQTSP
                     TTPYGANTTFTPPGKFIPEGKTLFPKDPPQLVIGGHPAVKYVATTCVSYPVDLMNKVR
                     KALNVDGPTFLHMLAPCPKGWKFDEKLTREMGRLAVETGMWTLFEWENGAYKYSHIPK
                     EYKPVGEYMKFQGRFSHLKPEHIAKMQAFVDEKIQRVKAIAVEAAVPTPKARA"
     gene            5231..6574
                     /gene="oxlT"
                     /locus_tag="VEL68_21125"
     CDS             5231..6574
                     /gene="oxlT"
                     /locus_tag="VEL68_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HXZ38545.1"
                     /translation="MPNEGMVQTDIAKQLYGPIAGNRWVQLIAGVIAMIVISNYQYAF
                     TLFTPGMRQQFTGVPYAQIALIFSMFILFETWPVPVAGYFVDRFGIRKLMILGSIGIL
                     VGWFLGGTVAKSVSDLYIYYGVIAGTGAGIIYISTVANAVKWFPDRRGLAAGLTAAGF
                     GGGAAITIMPIAATISSMGWAGAMATWGLAQGIVAFIMALILRHPPKGWAPPGWDQKA
                     LKVTKAVAQSKVEYTWTQTIRRPEFYLLYAMFLFVCSGGLMTTGNLSQIAKSLNVADA
                     KIWGMAIIPFTATLTGITNAFSRIMWGSISDKMGREYTMTLAFALEGFLIFMMTQIAG
                     NSIAFVILLPFVFLSWGEIYALFSAITGDIFGPKNASANYGILYTAKGAASILAGYGA
                     ALLAAYFAGSFTVPYYIAAAFDITAAILAFFALRPLVRGRIAKEVPKAAEALKPA"
     gene            6705..7634
                     /locus_tag="VEL68_21130"
     CDS             6705..7634
                     /locus_tag="VEL68_21130"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ38546.1"
                     /translation="MRILIAGGAGDVGRHLTEDFSRRGDEIRVLDRAPRRGEIEGPIQ
                     YVQGDLSDRECVQDALKGCEAVIHLAWSFSDDPQVVMGEDIRGHLNLLEAASAGGIKS
                     FVYASTATVYGRAAQHPVTENHPCLIVEARKPIYALGKYVSEELCRVYHRERKLPITI
                     FRFWWAFGESIGGRNLRDLIGKTLKNQPVELVRGAGGAFLSMGDLAKAVKMAMANPAA
                     SGQTYNLGTFFLTWEEIATRLIALAGSKSNLQFIPSEQWRGPAFLNEVWDLSWEKAGE
                     NLQYEPTEVSTALESQFMNALRDCLNLVKQGKA"
     gene            complement(7801..8463)
                     /locus_tag="VEL68_21135"
     CDS             complement(7801..8463)
                     /locus_tag="VEL68_21135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXZ38547.1"
                     /translation="MSDPYGRSSRFYNPIVEPWLRSIKERIVQECLHLSLSRVLDVGS
                     GTGTLVGMLQKEGIRAVGLDPSPGMLQVSRGNSQGSFALVRGRGERLPFSSGSFQGVV
                     FSMVIHENPPSRRRELLEDALRVLDPRGTLFILDYHRPSTVSGQAARMIEYLVEWVVG
                     GEHFRNYRQFMEEGALSGFLAHIPKERIHWQSAFHGSMSIACYRPGGFSPGGKDWVTT
                     SL"
     gene            complement(8694..9254)
                     /locus_tag="VEL68_21140"
     CDS             complement(8694..9254)
                     /locus_tag="VEL68_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38548.1"
                     /translation="MKKIIVDIDNTLWDLSPVLWEQLKTFNPSMPPPSRWNHWDFWEG
                     HLSLRDLFRALKIVHDHQDIYPPYPEAKPFLDALKGRGYYILIASHREKGTYDPTVRW
                     LDKHQLPYDEVHLMSDKSLLFDSCLAIVDDSPVTLDKAAHAGILRAGLLEPWNAGTGH
                     PLFSSLYEILDYLDSHISAGLCLKNR"
     gene            complement(9625..10323)
                     /locus_tag="VEL68_21145"
     CDS             complement(9625..10323)
                     /locus_tag="VEL68_21145"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXZ38549.1"
                     /translation="MTIDDVAKYLVRGHIVDLSKKVSPGKAEGPLDTGKRKYEIKMFT
                     FPPGELMHYIDMESHISTHIEAPSHFVPVRHPGKKGEDISELALTKFFGMGVLVDCKN
                     LPAKTPIGKEVLEKFEIKENDIVLIGRCRYQGADRCYMVKEGVEYLLQKKIKMVGVDD
                     TVFPENPLINRPPDLNKYFAHDLMLSNGIPIIEGLANLGELKKSRFLFFGFPAKMGGL
                     ESFPIRALAIEDKE"
     gene            10698..12011
                     /locus_tag="VEL68_21150"
     CDS             10698..12011
                     /locus_tag="VEL68_21150"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXZ38550.1"
                     /translation="MNRRDFLKAGSAILGAAAVGKALINPKEVKAAMGRTEKIKITDV
                     RTKMVAVPFARFGEFRPVTMWYMTRHASIHCITYIDTDAGITGVGTQGDQQVIMNVIR
                     PKLIGKDPFDIEKIENEELGGPGLGGRWRLFSTDTMAAIDGALWDIIGRTCNQPLYRL
                     WGGKVNEPIHLRYWLDCQSPEKQVEEAKKAVARGWKAFKVKLGTDPETDIERMKALRE
                     GLGDKIELCFDINGGYPLNVAINTLKKMAKYNPAAIEDPVPCIWPYDAGALDNMADIR
                     RITGIPIEAHSHGPNCEEFVIELVKKRAADTLHLNTSFVGSILECRRLCAIAEAGGLT
                     VTGQSSASELGPRNALLLHLYTAERSFQGTNDNSTHFLEPPSGDIIKNEFRVENGTLK
                     VPEGPGLGVELDEEKVAKYNEVYRSSEKYRKHEPGLGRKNSYLWH"
     gene            12298..>12704
                     /locus_tag="VEL68_21155"
     CDS             12298..>12704
                     /locus_tag="VEL68_21155"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXZ38551.1"
                     /translation="MGKLESTIKSEIQRLAKREIRSTFIPLRREVGAIRLKLSGLSRG
                     ITSLNRMTKELHLEEARPKLEATPEEVKVSRLTPDRIRGLRKKLGISMRELGTLTGTS
                     LSAILSWEKGKFKPRGEKKGALVALRKLRKREV"
BASE COUNT         3019 a         3393 c         3615 g         2677 t
ORIGIN      
        1 cggaggggcg tcgggcgatc ctgaaaatcc acagccgccg catgcctctc agccccgatg
       61 tgaacctgga tcgactcgcc cagatcaccc atgcctttgt cggggccgat ctggaggcct
      121 tgtgcaaaga ggccgggatg gtggccctgc gccggtacct agcgatggaa ggggagaacg
      181 acctggccag caaactggcg aattccgagc agctgcagat cgggatggaa gactttttgt
      241 tggccctgag ggaaatcgaa cccacggcca cccgggagat ctacacggag cgctccgcgg
      301 tgagatggca ccatgtgggg gggctgcgga aggtcaaaga gacgcttctt tccatcgtgg
      361 agtggccggc gcgctatccc gacctctatt ccgccggccg ggtcctgccg cccaggggga
      421 ttctcttctc cggcccctcc ggcaccggca agacgctcat ggccaaggct ttggccggcg
      481 aaaccggact caacttcatc agcatcagcg ggccgattct cttctccaaa tggctgggcg
      541 aatcggaaag ggcactccac cacatcttca agaaggccaa gcagtcggcg ccctgcatcc
      601 tcttcttcga cgagattgag gccctgggca tgacccgggg cacgggcagc gatagcgggg
      661 ccgtggagcg catggccagt caatttttcc acgaaatgga taacctgagc gacctttccc
      721 aggtcattgt cctgggggcc acgaaccgga aggacctcct cgacccggcc ctgacccggg
      781 cggggcgcct ggaattcgtt ctgcccttcc ccctgcccga cgaaagcgag cgcctggaaa
      841 tcttccagat tcatacggcc gaaaggcccc tggccccggg cgtggacctc agcgatctgg
      901 ccaggaggac ggaagggatg agcggagccc aaatcgctca tacctgccgg acggcagccc
      961 tggccgccat cacggagatc gtgaagggac cggaggaggc ccggtcgcag aaactctccc
     1021 ttcgagcgtc ccacttccac ggggcgatcg aagaatgggg gaagaaggag tctccgccat
     1081 gctgagggaa gtgagaaaaa agaaggccca tgaggatatc gtcaaacaga tccgttctct
     1141 ggtggaaaag aagatgttga aacaggggga gaagcttcct cccgaaagag acctctcgga
     1201 gaccttcaaa gtctcccggg ccacggtgag ggaagccatt ttttccctgg agaccatgaa
     1261 gctcgtgaga cggcgtcagg gggacgggac ctacatcacc gccgcccgcg gggaagacct
     1321 ggtgcagccg ctggctctct ccctggtcca tgagaaggac gacctcatcg acattttttc
     1381 tttgcgcaag ctgatcgagc cggaagtagc ccgtctggcc tcggataacg cccacccgga
     1441 agacatccag cacctggaaa gaatcctccg tgagcaggag cgggaggtga accggaagag
     1501 gagcgcgctg cggatcgact cggaattcca tcaggccctt gcccagacgt cccgcaacca
     1561 gatttttgaa cgcctgctca tggcggtcat ggatcttcta cagaagaccc gggagcaata
     1621 tttccagacc gaggaaagga aacgaatttc tctgatgggt caccgggata ttctgggcgc
     1681 catccgggcg cgcgacggga gaaaagcccg gctggccatg cgccggcatt tggaaagagt
     1741 ggaaagaatt gcattcaaga ggggaggtga agagaagtca taagctctca cccgggtaca
     1801 gggtgagaat ggatcgtccg ggcctgccgg atcgaggaac ggattaactc atgtcaagga
     1861 ggaaggaagg tatggcaaaa gttacggaaa taaccgtatg gacgcggggc gtggtcatgg
     1921 acaaggaggc ccgcgatgtg gccaacgcct ttgcccaagc cgccaaaacg gaaggaaagt
     1981 tcattcagtc cttcgacaat tacgaggacc tgcccgaccg ggtcctggtc accacccgga
     2041 aatacgcccg catcagcgat gaagagatcg ccatgaagta cgagtacgcg aaccaccatc
     2101 ccgacgtggt ggtgatcgtg gaacccacga tcgtcaaagg aatcaacgtg ctgaaaggca
     2161 tgcccgaagg ggggcttttg gttatcaata ccagccgccc gatcgattac atgctgaaat
     2221 tcatccccaa cgccgacgtg ttgggagcga tcgccacggt agacgccgat ggcatctccg
     2281 gagtccgcac cgtggatttt tccggatccg aaggcggggt ggacgcagcg ggaatcggat
     2341 cgggtatcgc cgcccccatc ctgggcgcca tggcccgggt cacgaacatg gtgaaaaagg
     2401 agagcctgac caagatcgta aaagatgtgg cctccctgga gcgcgggttc aaggaagtca
     2461 aactccagaa attccgcaag tcccgcgctg aatatttctg ggggagctga gaacatttcc
     2521 attttcaagg gatccaatgg aaaggagaaa agaatgccca ttaaagttcc caaagatcga
     2581 gaagtcccct tcggagccat cactccggct ccggtcaagc agcaggaact cttcatcact
     2641 tcgaactggc gtatcgtccg gccggtcatc gaccatgaaa aatgcacccg ctgcttcacc
     2701 tgttacgctt cctgtccgga tgcttgctgg tcgttcaatg agaaggaaga aaaaatggaa
     2761 tggaactgga agttctgcaa gggttgtcag atatgtatct atgagtgccc agcagatgcc
     2821 ataagcgccg ccccggagct tgatttcgaa gacggggtgg tccgcttgga aaaacctttt
     2881 taagaaggag gaagatgatg gctcaagaaa gaatgctcag cggctgcgcg gcggcagctc
     2941 aaggggcgaa gttggccgaa gtcgaggtca tctgctcatt tcccatccgc ccttacacgg
     3001 cgatcatgat ggaactggcc cggatggtgg ccaatggaga gttggatgcc gagttcatcc
     3061 acggggaagg agaacacgcc caggtatcgg tcgtcctggg ggcctccgcc gccggtgccc
     3121 gagcttacac gggcagctcg ggcgtggggg taacctatgc gtttgaagtc tacagcccgg
     3181 cggcgggcgg ccggtatccc ctgcagatgg ccatcgccga ccggaccctg gatcctcccg
     3241 gagatttcgg ctcggaacac acggatgcca tgtccgcccg ggaccagggg tggctgatgg
     3301 ggtgggcctc cactccgcag gaagtgctgg acaataccct gatcaatttc cgcgtggggg
     3361 aagatccgga gatcatgctg ccccagttcg tgtgccagga cgggtacttc ctctctcata
     3421 tcccggataa agttgtcctt ccggaaatgt cccaggtgcg ggagttcctg cccccgtaca
     3481 aggctccgca ccccttgagc acggagtgcc cggtttccca tggcccgcag attcgtccgg
     3541 accagggggc gcccatggat gcccaacggg cggccacgtt cctggaagtc ccgcgggtga
     3601 tcgaaaaagc ggtggatgat ttcaaccgga tcttcaagcg taattacgac ccctacctgg
     3661 aaaaatacaa gatggaagac gccgaggtgg cctatttcat ccagggggcc catgccaata
     3721 cctgcaaagc agcgatcaac cagttgcgga gcaaggggtt gaaggccggg atggtccggc
     3781 cgcgctgggt ccggccctgg ccgacccagc agattgccga agctttcagc aaggtcaagg
     3841 ccatagcttc ggtggagacc agcacctcgt acggcggggc gatgcggggc ggaaacctgg
     3901 ttcacgaatt gcgggcctcc ctctacgacc tggagaagcg gcccctggtc acctctttca
     3961 tggccggtct gggcggagac gtcatcctcc tggaggattt tgactacatg gccaagatcc
     4021 tgacccagat gatccaggaa aagaagaccc gccagcacgt gtactggatc ggtttcgaag
     4081 aataataacc cctttccaac tcaggaggta tttcctatgg ctgaaggatt tgattataaa
     4141 aaacagattt tggagccgat caagagcatc cgcagcgccc cggcggaaga gatgtatctt
     4201 ccgggccacc gaacctgcgc cggatgcggg ccggccctga actaccggct gacggccaag
     4261 gccgccggca aggacacgat tttcatcggg ccaacgggct gcatgtacgt ggccaactcc
     4321 agctacctct gcacccccta tgccgtttcc tggatccatg cccagatcac caacggcggg
     4381 gccgtggctt ccggaatcga ggccgcctat aagattatgg cccgaaagaa gaaatacacc
     4441 ggcgccttcc cgaatgtgat cgtcatggcg ggcgacggag ggtccgtgga tatcggcctg
     4501 caggccatgt cggcgatgat gtaccgagcg cacgatatcc tcttcatctg ctacgacaac
     4561 gaatcctacg ccaatacggg gattcagact tcgccaacca ctccgtacgg ggccaatact
     4621 acctttacgc ccccgggcaa gttcattccg gaggggaaga ctctcttccc caaagatcct
     4681 ccgcagttgg tcatcggggg acatccggcc gtcaagtatg tagccacgac ctgcgtttcc
     4741 taccctgtgg acctgatgaa caaggtccga aaggccctga acgtggatgg ccccactttt
     4801 ctccacatgc ttgccccctg cccgaaaggg tggaagtttg atgaaaaact gacccgggaa
     4861 atgggccgcc tagcagtgga gaccgggatg tggaccttgt tcgagtggga gaacggggcg
     4921 tacaaatact cccatatccc caaggagtac aagccggtcg gcgagtacat gaaatttcag
     4981 gggcgcttct cgcacctcaa accggagcat atcgccaaga tgcaggcctt cgtcgacgag
     5041 aagatccagc gggtcaaggc gatcgcggtc gaagcggcgg ttccgacccc gaaggcaaga
     5101 gcctgaattt cggcgaaaac agaagaagat ggttggaatg ctggcccgga cggaaatccc
     5161 ccgccaacag ggaaggggat ttccctgggc ctacgggttt ggcactttaa ccttctgaaa
     5221 ggagagcggt atgcccaacg aaggcatggt tcaaacagac atcgccaaac aattatacgg
     5281 gccgatcgcc ggcaatcgat gggttcagct gatcgccggc gtcatcgcca tgatcgtcat
     5341 atcgaattat cagtacgcct tcaccctctt caccccgggg atgagacagc agttcacggg
     5401 ggtgccctac gcccagatcg ctctgatctt ctccatgttc atcctgttcg aaacctggcc
     5461 ggtgccggtg gccggttact tcgtcgaccg gttcgggatc cgcaagctca tgatcctggg
     5521 ctctatcggc atcctggtgg gatggttcct ggggggcacg gtggcgaaat ccgtctctga
     5581 cctgtacatc tattacggcg tcatcgccgg gacgggggcg gggatcatct acatctccac
     5641 ggtggccaac gcggtcaagt ggttcccgga ccggaggggg ctggccgccg gactgacggc
     5701 cgccgggttc gggggcgggg cggccatcac catcatgccc atcgcggcca cgatcagctc
     5761 catgggctgg gccggcgcca tggctacttg gggactcgct caggggatcg tggccttcat
     5821 catggctttg atattacgtc acccgcccaa aggttgggcg cctccggggt gggatcaaaa
     5881 agccctgaaa gtcaccaagg cagtcgctca gtccaaggtg gagtacactt ggacccagac
     5941 gattcggagg cctgagtttt accttttgta cgccatgttc ctctttgttt gcagcggggg
     6001 gttgatgacc acagggaatc tgtctcaaat cgccaagtct ctcaatgtgg ctgacgcgaa
     6061 gatatgggga atggccatca ttcctttcac cgcgaccctt acaggaatca cgaacgcctt
     6121 ctccaggatc atgtggggat caatctccga caagatggga agagaatata ccatgaccct
     6181 ggcctttgcc cttgaaggat tcctcatctt catgatgact caaatcgccg gcaactcgat
     6241 cgccttcgtt atcctgttgc cctttgtgtt tctctcctgg ggcgaaattt acgccctctt
     6301 ctcggccatt acgggtgaca ttttcggacc caagaacgcc tccgccaatt atgggatctt
     6361 gtatacggcc aaaggggcgg cctccatcct ggcaggatac ggggccgcgc tgctggccgc
     6421 gtatttcgcc gggtctttca cggttcctta ttacatcgcc gcggccttcg acataaccgc
     6481 cgcgatcctg gctttcttcg ccctgcggcc cctggtaagg ggcagaatcg cgaaggaagt
     6541 tcccaaggca gcggaagcct taaaaccggc ctaaggagag ttgaagcccc gggcagagac
     6601 aatgaatcca ttgcctctgc ccgggcagtt ttagggggtt cttcctgggt gagacggctg
     6661 atgccgttcg ggatagcctc ccctcttctt ctttggagca agccatgcgt atcctgattg
     6721 ccggtggtgc aggcgatgtc ggccgtcacc tgaccgaaga tttttccagg cggggagatg
     6781 aaatcagggt cctcgaccgg gcgcctagga ggggagagat cgaaggaccc attcaatatg
     6841 tgcaaggtga cctaagcgat cgagaatgcg ttcaggatgc gctcaaggga tgcgaagcag
     6901 tcatccattt ggcctggagc ttttccgatg atccccaggt ggtaatgggc gaagatattc
     6961 ggggccattt gaacctcctg gaggccgctt ctgccggggg aatcaaaagc tttgtctatg
     7021 cgagcacggc cactgtttat gggagggccg cgcagcaccc ggtgaccgag aaccaccctt
     7081 gtttgattgt ggaggcccgg aagccgatct atgcgctggg caaatacgtg tccgaagagc
     7141 tatgccgggt ttatcaccgg gagcgcaagc tcccgataac cattttccgc ttctggtggg
     7201 cttttggaga gagcatcggg ggccggaact tgcgggattt gatcggcaag actttgaaaa
     7261 atcaaccagt ggaattagtt cgaggcgccg ggggagcttt cttaagcatg ggagacctgg
     7321 caaaagccgt aaagatggcg atggccaatc ctgcggcttc aggtcagact tacaacttag
     7381 gtactttctt cttgacctgg gaggaaatcg ccacaaggtt gattgccctg gcgggttcga
     7441 agtcaaacct tcagttcatc ccttccgaac agtggcgagg cccggccttc ctgaacgagg
     7501 tctgggactt gagctgggaa aaagcgggag aaaacctgca gtatgagccg acggaggtgt
     7561 caacggccct tgagagccaa tttatgaatg ctcttcggga ttgcttgaac ctagtcaagc
     7621 aaggaaaagc ttgaacctcc aacttccgaa gtgcggatcc cgagacggtt attattctct
     7681 ccctcaattc gactgtttgt cgttcttttt tgccattgcg agcgatcccg ccatggcggg
     7741 agagcgtggc aatctcattg atttccaaat tttatgagtt gcttcgatcg ttcccctctc
     7801 tcacaatgat gttgtgaccc agtctttccc gccaggggag aaacctcccg gacgataaca
     7861 ggctatcgac atggacccgt gaaacgcgga ttgccagtga attctttcct tgggaatatg
     7921 ggccaggaaa ccggataggg ccccctcctc catgaattgc cggtagttgc gaaagtgctc
     7981 ccctcctaca acccattcga ccaggtattc gatcatcctc gcggcctgcc cggaaaccgt
     8041 cgagggcctg tggtagtcaa ggatgaagag cgtcccccgg gggtccaaaa cccgcagggc
     8101 gtcttccagg agctcccttc ttcgggaagg cgggttttca tggatgacca tcgaaaaaac
     8161 caccccctga aaagaccccg aagaaaaggg aaggcgctcc ccccggccgc gaaccagggc
     8221 gaatgatccc tgagaattcc cgcgggatac ctgaagcatt cccggggatg ggtccaggcc
     8281 tacagcccga atcccctcct tctgcagcat tcccaccagt gtccccgtgc cggaacccac
     8341 gtccagtacg cgagaaaggc tgaggtggag gcattcctgc acgattcgtt cttttatgga
     8401 ccgaagccag ggttcgacga tgggattgta gaaccgggag gatcgtccgt aggggtcgct
     8461 catggggaat gattcctttc gaattttcga aagacccgcg cgggcggtca aaataagaaa
     8521 acttcaaatt ttccaaggaa tcttgtttgg attacccgtc tctgaaatcc cccccaaccc
     8581 ccctttgata aagggggggc aggggggatt tctggcaggg ccgtggtact agggaaaatc
     8641 ctggctgcta ggtccggctt gccaatctgc gaaaattcgc cagaagtgat tgatcacctg
     8701 tttttcaaac aaagcccagc tgaaatatgg gaatcgaggt aatccaggat ttcgtaaagg
     8761 ctcgaaaaca aggggtgccc ggtgccggcg ttccagggct ccaggagacc tgcgcggagg
     8821 attccggcgt gggccgcctt gtccagggtg accggactat catcaacgat ggccaggcag
     8881 ctgtcaaaga gcagggattt gtcgctcatt aggtgtactt catcataggg gagctggtgc
     8941 ttgtccagcc atcggacggt ggggtcgtac gtccctttct cccggtggct ggcgataagg
     9001 atataatatc ccctgccctt cagtgcatcc aaaaagggtt ttgcttccgg gtaaggagga
     9061 tagatgtcct gatggtcgtg gacgattttt agcgcccgga acaggtccct cagggaaaga
     9121 tgtccctccc agaaatccca gtggttccat cgcgacggag ggggcatgct cgggttgaag
     9181 gttttcagct gttcccatag cacgggggac aggtcccaga gggtgttgtc tatatcgacg
     9241 atgatttttt tcatttctct cccaaccaga ttgagccgga atcaaaaaga aaatgtatgg
     9301 gcggccccct gtggccgccc acaaaaaaag caaccgcaga tgattgcccc tgcacgatag
     9361 ttcccatagt atgcagtagg ggcgggtttc aaacccgccc ctatcagctt tatccaattc
     9421 tctcactggg gaaaaaaagg agttatgatt ctccgcatcc ctatcaaaat cattgaaata
     9481 ttttagccgc tcgataatgg tctggatttt ctgtaagtag ataaaaatac tgggcaaaag
     9541 ccaccattgt ctactccagc taattctatc tgttccggga tccgacttct gaacaagctt
     9601 cctcggcgtt caaaggatac actcctattc cttatcctca attgccagag cccgaatagg
     9661 aaaagattcc aggccgccca ttttcgccgg gaaaccgaag aacaggaatc tcgacttctt
     9721 cagctctccc agattggcca acccctcaat gatgggaatg ccgttagaaa gcatcagatc
     9781 atgagcgaag tatttattca aatccggagg acgattgatg agcggatttt cagggaacac
     9841 cgtgtcatcc actcccacca tcttgatctt cttttgcagg agatattcca caccttcttt
     9901 taccatataa caacgatccg ctccctgata ccggcatctt ccgatcagaa caatgtcatt
     9961 ttctttaatc tcaaattttt ccaggacctc ttttccaatc ggagttttgg cgggcaggtt
    10021 cttgcagtcg accaaaaccc ccattccgaa aaatttggtt agggctaact cgctaatgtc
    10081 ttcccctttc ttccctggat gccgcacggg gacaaagtga gatggagcct ctatatgggt
    10141 ggagatatga ctctccatat cgatgtagtg catcagctct cccggcggaa aggtaaacat
    10201 tttgatttca tacttgcgtt ttcccgtatc gaggggaccc tccgcttttc ccggtgaaac
    10261 cttcttggaa aggtcaacga tgtgccctcg gaccaaatat ttcgccacgt catcaatggt
    10321 catgaaagcc tcctttattt gtcatttctc ctcgatagcg ggagtatatt ggaaaaggga
    10381 tggggaggca aggggaatca tgggagaacc cttcagatca gggtgttttg catgcctagt
    10441 ccgctttcca taaggcatta ggaaatggtc aaaaatttct tgaccttgct ccctcaaatt
    10501 catataatcc caaccggatc aactagtcgg cattcctgtc cttatccatc aaccctagcc
    10561 ttgactcttt cccggtggcc aggaggttga atggtttggt aacttggata gaccccattt
    10621 ctgaggaaga gatggggttt tttatttgga aaagaacaaa acgggacccg caaatccata
    10681 gaaaaggaga cgaaagaatg aatcgtcgag actttcttaa agctggttcg gcaatattgg
    10741 gagcggcagc agtaggcaag gcgttgatta atccgaagga ggtaaaggca gcaatgggac
    10801 gtaccgagaa aattaaaatt accgatgttc gcaccaagat ggttgcggtt cccttcgccc
    10861 gatttgggga gtttagacct gtaacgatgt ggtatatgac ccgccatgca agcattcact
    10921 gtatcaccta catcgatact gacgccggga tcaccggggt cgggactcag ggcgatcagc
    10981 aggtaatcat gaatgttata aggcccaaac tcatcggcaa ggatcctttt gatatcgaaa
    11041 agattgagaa tgaggaattg ggaggaccgg gcctcggcgg gagatggcgg ttgttctcga
    11101 ccgatactat ggcggcaata gacggggccc tatgggatat catcgggaga acctgtaatc
    11161 aacccctcta tagattgtgg ggaggaaagg tgaacgagcc cattcatctc cggtattggc
    11221 ttgattgcca gagtcctgaa aagcaggtgg aagaagctaa gaaagccgtt gcccgaggct
    11281 ggaaggcctt taaagtaaag ctggggacag acccggaaac ggacattgag aggatgaaag
    11341 cgttgagaga gggactagga gataaaattg aactttgttt tgacattaac gggggttacc
    11401 ccctcaatgt agccattaat acgctaaaga aaatggctaa atataaccca gcggccattg
    11461 aagacccagt cccctgcatt tggccttatg acgctggtgc attggataat atggctgata
    11521 ttaggaggat aacgggaatt ccgattgagg cccacagcca tggccctaac tgtgaagagt
    11581 tcgtaattga actcgtgaag aagcgagcgg cagataccct ccacctgaac acttctttcg
    11641 taggctccat tttagagtgt cgccgtctct gtgccatagc tgaagcagga ggccttaccg
    11701 taaccggtca aagcagtgcg tccgagctgg gccccagaaa cgccctatta ctccatcttt
    11761 atacggccga aagatctttt cagggtacaa atgacaactc cactcatttc cttgaacccc
    11821 catccggcga catcattaag aatgagttcc gggttgagaa tgggactttg aaggttcctg
    11881 agggaccagg cttaggggtc gaactggatg aggaaaaggt agccaaatat aacgaggttt
    11941 acaggtcttc ggaaaaatat cgaaaacacg agccaggttt aggaagaaaa aattcctatc
    12001 tctggcatta aagcaatcag ggttttggtc attaataagc atcgccaaaa tgccgtaagc
    12061 acatttgaaa catttgtgaa agccactata gagaacgaaa ctaaaagcgc tgtgcttctg
    12121 caggcaactc agtgtatatt ctcgcaacag agcagtggat acattgcaga ggaaagcgat
    12181 tcatcaatca accctcaatg gatggagatt atcaagggaa ttttcaacat tccaaaatac
    12241 aaaagactac attgagaggg aaatagccgg tccaaatttc agactccgga ggcctccatg
    12301 ggcaaacttg aatccaccat caaatctgaa atccaacgct tggccaaaag agaaatccga
    12361 tctaccttta ttcctctccg cagagaagtt ggagccataa gactcaagct atctgggctc
    12421 tccagaggta tcacttcttt gaacaggatg accaaagagc tgcatctgga ggaagccagg
    12481 ccgaagctgg aagccacccc agaggaagtc aaagtatctc gccttacccc tgaccggatt
    12541 cggggactca ggaaaaagct gggcatatcc atgagagagt tgggcaccct tacggggaca
    12601 agcttaagtg cgatcctatc ctgggagaaa gggaaattta agcccagagg agagaagaaa
    12661 ggtgctctgg tggctctgag gaagttgaga aagcgggaag ttag
//
LOCUS       DASPRR010000575         5201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2078536, whole genome shotgun sequence.
ACCESSION   DASPRR010000575 DASPRR010000000
VERSION     DASPRR010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078536"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VEL68_21160"
     CDS             <1..599
                     /locus_tag="VEL68_21160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38552.1"
                     /translation="SEGYVPPDALTWTDADNNVNFNNKTVVMTPNPSISIPGAHFFTN
                     KDNYYNKIGSILQPTSPVDGKGYPYLVSVKPIIIPMDSKQQALAKKFISYVLETKNFT
                     SYVTASNGRWFPSFKDVAADPFFTNPKDPHVPVATDQHLNHETRPFYYSYHPAYSQVY
                     TENVWGKAMSRVVTEKWTNEKAADEAIDRIQTIFKSWK"
     gene            667..1536
                     /locus_tag="VEL68_21165"
     CDS             667..1536
                     /locus_tag="VEL68_21165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ38553.1"
                     /translation="MSQATPVPVASPSTLHPEQWGRKTVLGIAFCSPLIMIFLCLVIY
                     PMLYEIYLGLDWDIYTSIFSDPIFIQTLWNTVIFVGLAVNIKMVLALTLSSLFSTDHR
                     FTKILGGIFLLPWAIPSLPGILGFRWMLNGQWGIVNKILNDFGFEGYPWLVRRPTALA
                     AAITYHIWKYLPFWTLIFVAGRRGIPKELYEAGDIDGTSPLQKFRYITFPMLRNLYLI
                     CILLSMVFTLGDFVIIKIMTGGAPGDSTHVLSTLAYRYTFQMGKIDWGVGTFLTALPI
                     TLLFIFILIRKVK"
     gene            1545..2387
                     /locus_tag="VEL68_21170"
     CDS             1545..2387
                     /locus_tag="VEL68_21170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ38554.1"
                     /translation="MRLRGWRKLAFYTVCSLLLVWTIFPVYYMITLSLVPEEDLFKPR
                     LYVAQPTLDNYRQTTQQSNYLVSEFWIQLYNSVIIALLTTAAVVIVGTLGSFALARIR
                     FRGSQIISSMTLFTYIIPASFLSIPFFKLMADYDLLGTKLSVILAMITFTSPYAVWIL
                     WDYGKTVPPEIDEAAAIDGAGMLQTFFRIYLPLILPPMVAIATYAFLFSWNEYLYVVL
                     FLSGETNITIPVAMGNFLATDNPPWNLLMAESVLYSIPPVILYYCFRRHLLSGLVTGS
                     VTGT"
     gene            complement(2557..3111)
                     /locus_tag="VEL68_21175"
     CDS             complement(2557..3111)
                     /locus_tag="VEL68_21175"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ38555.1"
                     /translation="MSGTESKPKGIIGRLEAGSGFLGQLGLIFMVVSITYDVVLRYVF
                     VAPTNWALEVNTFLLAFLCVIPAGEVLRVGGQIRITFLLDRLSPAVKDRLDILRAVVG
                     LFFCGIMIWKGADMAWTAWQHNDRMSTSLGTPMVIPYLFLPVGFCLLALQYVALLAAR
                     RPKPPRLPGEGTSLGKPPEVEQQI"
     gene            complement(3108..4148)
                     /locus_tag="VEL68_21180"
     CDS             complement(3108..4148)
                     /locus_tag="VEL68_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018403859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXZ38556.1"
                     /translation="MRGFTRRQFLKAAGVGAGTALLSGAGIRGTAQGAPPTFTGVTYL
                     TPAYENSFPIIMGFVDQLKKHPDLFKIDFFDSGTLIKADEQTAALRAGTIHFMFHTTS
                     YITRTFPVLGITGLPSLCGQLAVHGERLDMESPLWKQINDVLAKDNTFMLTAGGGVEE
                     PEYIWSGANKVSSLADLKGKRVRIVSYEAEEVLKAYGVAGARIPSSELYLAVQRGTVE
                     ATVANISTVVGRKLYEQVKNCYQLPVTAFAIAIFFMKDRWDKLPDNIKAAFWDAGKWF
                     DKNYARVLNTKFYPENYWPKVQKAGVKIYKPTETELKDFEVKSQPVWAWWKKQVGEDA
                     GQKAINFALGRT"
     gene            complement(4177..>5201)
                     /locus_tag="VEL68_21185"
     CDS             complement(4177..>5201)
                     /locus_tag="VEL68_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ38557.1"
                     /translation="GGLAIASIGACTVFGAVSGVSVAGVAAIGSMAVPEMLKRGYSTK
                     IAAGSVVTAGALAMLIPPSVPFIIYSAITGESVAKLFIGGIVPGLVLAAMLSIYVFFR
                     VLITPELAPRSAELKFSWRERWTSLARVWHTGILIFLVLGSIYLGVATPTEASALGAI
                     GAFLIAGLVYRNMNFRRIVQIIRESMMISVAILLIMGCAKIFGDYLNMVRVPEMVTSF
                     FMGLNMPKLVMVLLIQGILIIGGMFVDAGSLMVITTPILLPLIKAMGYDPLWYGIVLV
                     INLELAVVTPPVGLNLYTLRSCCPVLTLEEIIKSSLPFLVIQLICLILFSVYPPLSLW
                     LPNLIN"
BASE COUNT         1105 a         1552 c         1383 g         1161 t
ORIGIN      
        1 acagcgaagg ttacgttcct ccggatgcgc tgacctggac cgatgcggac aacaacgtca
       61 atttcaacaa caagacggtg gtgatgactc ccaacccgtc tatttccatc ccgggggccc
      121 atttcttcac aaacaaagat aactattaca acaagatcgg gtccatcctc cagcccactt
      181 ccccggtgga cggaaaaggg tatccctacc tggtatcggt gaaaccgatc attatcccca
      241 tggattccaa gcaacaggcc ctggccaaga agttcatctc gtatgtactg gagaccaaga
      301 acttcacctc ctacgtgacc gcttccaatg gccgctggtt cccatccttt aaggacgttg
      361 ccgcggaccc cttcttcacc aatcctaaag acccccatgt gcccgtggct accgaccagc
      421 atctgaacca tgagacccgc cccttctact acagctacca cccggcctat tcccaggtgt
      481 acaccgaaaa tgtctgggga aaggccatgt ctcgggtagt gaccgaaaag tggaccaacg
      541 aaaaggccgc ggatgaagcc atagaccgca tccagacgat tttcaagagc tggaaatagc
      601 cgttccttaa aaaaatcgaa cctgtttggg gggctccgag ccccccaact ttcatgagtt
      661 attctaatgt cccaagctac acctgtccct gtcgcatcgc catcgaccct tcacccggaa
      721 caatgggggc ggaagaccgt tctggggatc gccttctgtt ccccgctcat catgatcttc
      781 ctgtgcctag tcatttaccc gatgctctac gagatctacc tgggcttgga ttgggacatc
      841 tacacctcca tcttctcgga cccaatcttc attcaaaccc tttggaatac ggtgatcttt
      901 gtggggctgg cggtgaacat caaaatggtc ctggcgctga ccctctcatc cctattcagc
      961 accgaccacc ggttcaccaa gattctggga gggatcttcc tgcttccctg ggccattccc
     1021 tctcttcccg ggattttagg ctttcgttgg atgctgaacg gccagtgggg aattgtgaac
     1081 aagatcttga acgatttcgg tttcgaaggc tacccctggc tggtgcgccg gcccacggcg
     1141 ctggctgcgg ccattaccta ccacatctgg aaatatcttc ccttctggac cttgatcttt
     1201 gtagccggac ggcgtggaat tcccaaagag ctgtacgagg cgggcgacat tgacgggacg
     1261 tcgccgctcc agaaattccg ctacattacc tttcccatgc tccggaacct ctacttgatt
     1321 tgcatccttt tatccatggt tttcaccctg ggagacttcg tcatcatcaa gatcatgacc
     1381 gggggagcac ccggtgattc gacccatgtc ttgtctacgc tggcttatcg gtatactttt
     1441 caaatgggca agattgactg gggagtgggg actttcctca ccgcgctgcc cataaccctg
     1501 ttgttcatct ttatcttgat ccgcaaggtc aagtaggagt ttttatgcgg ctccgcggct
     1561 ggcgaaagct cgctttttac actgtttgca gtctcctcct ggtgtggacg atcttcccgg
     1621 tgtattacat gatcaccctt tccctggtgc ccgaggagga ccttttcaag ccccgccttt
     1681 acgtggccca gccaaccttg gataactacc gccaaacaac ccagcaaagt aactacctgg
     1741 tcagtgagtt ctggattcag ctctataaca gcgtgatcat tgccttgctg actactgcgg
     1801 ccgtagtcat cgtgggtacc ctgggcagct ttgccctggc ccggatccgc ttccggggca
     1861 gccagatcat atccagcatg acgcttttca cctacattat tcccgcctcc tttttatcca
     1921 tcccgttctt caagctcatg gccgactatg atctgctggg aaccaagctg tccgtcatcc
     1981 tggcgatgat cacttttacc tcgccctatg cggtctggat cctctgggac tatggcaaga
     2041 ccgttccgcc ggagatcgat gaagccgcag ccatcgatgg agcgggaatg ctgcagactt
     2101 tttttcgcat ctaccttccc cttattcttc cccctatggt tgcgattgcc acttatgcct
     2161 ttctgttttc ctggaatgaa tatctatacg tcgtcttgtt tctttcgggt gaaaccaata
     2221 tcaccatccc ggtggccatg ggtaacttcc tggccacaga caaccccccc tggaacctcc
     2281 tcatggccga atcggtcctc tactcgattc ccccggtcat cctctattac tgcttccgcc
     2341 gccatctcct gagcgggttg gtgaccggct ccgttaccgg gacctaaatg ccctgccctc
     2401 cgaagggggc tcatcgaatc attccctttg gagcgttgaa aaccaccgaa agcaaaaagc
     2461 tcggaggaag tcccccgagc ctttttcccg gcagagagtt tcctccctta ccgtgggccg
     2521 acatcagcga ccgaattgaa ttcttccttg cgtctcctag atctgctgct cgacctccgg
     2581 gggcttaccg agagaagtgc cttcgccggg aaggcgtggg ggtttcggcc ttctggctgc
     2641 caaaagggca acatactgaa gggccagaag acaaaaaccc acggggagaa aaagataagg
     2701 gatcaccatg ggcgttccca gggacgtgga catgcggtcg ttgtgctgcc aggccgtcca
     2761 ggccatgtcc gcccccttcc agatcatgat cccgcagaaa aagaggccga ccacggcccg
     2821 caggatatcc aggcgatcct tcacggccgg gctaaggcgg tcgagaagaa aggtgatccg
     2881 aatctgcccg ccgacgcgca ggacttctcc tgcgggaatg acgcacaaga aggcgaggag
     2941 aaaggtgttc acttccaggg cccagttggt gggagcgaca aagacatagc gcaggaccac
     3001 gtcataggta atgctcacca ccatgaagat caggcccaat tggcctagga agccgctgcc
     3061 tgcttccagc cggccgatga tcccctttgg tttgctttcc gttccgctca tgtccgcccc
     3121 agggcaaagt tgatcgcctt ttggcccgca tcctcgccga cctgcttctt ccaccacgcc
     3181 cacacgggct ggctttttac ctcgaagtct ttgagctcgg tttcggtagg cttgtagatc
     3241 ttgacccccg ccttttggac cttgggccag taattttccg ggtagaattt cgtattcagc
     3301 acgcgggcgt agttcttatc aaaccacttg cccgcatccc agaaggcggc tttgatattg
     3361 tccggcagct tgtcccaccg gtccttcatg aagaagatgg cgatggcgaa agcggtgacg
     3421 ggcaactgat agcaattttt gacttgttcg tagagcttcc ggcccactac cgtgctgatg
     3481 ttcgccaccg tggcctccac ggtgccccgc tggacggcca ggtaaagctc ggaggaggga
     3541 atccgggccc cggccacgcc ataggccttg aggacttctt ccgcctcata gctgacgatc
     3601 cggacccgct tgcccttcag atcggccagg ctggagactt tgttggctcc gctccagatg
     3661 tattcgggtt cttccacccc ccctccggcc gtgagcatga aagtgttgtc tttggcgagg
     3721 acatcgttga tctgcttcca cagcggcgat tccatgtcca gcctctcccc atggacagcc
     3781 agttgaccgc acaggctggg aaggccggtg atgcccagga cgggaaaggt ccgggtgatg
     3841 taagaggtgg tgtgaaacat gaaatggatc gttccggcgc ggagggccgc ggtttgttca
     3901 tcagctttga tcagggttcc ggaatcgaaa aagtcgattt taaacaggtc tgggtgcttt
     3961 ttgagctggt ccacaaaacc catgataatg gggaacgaat tttcgtaggc gggagtcagg
     4021 taggtcaccc cagtgaaggt ggggggagct ccctgggcgg tgcctcttat gccggccccg
     4081 ctcaacagag cggtcccggc gcctactcct gcggccttga gaaactgacg tctggtgaat
     4141 cctctcatgg ataacctcct tcgggcgcat ccgcccttaa tttatcaaat ttggcagcca
     4201 cagactcaaa ggcgggtaga ccgaaaaaag gatcaaacag atcagctgga tgaccaagaa
     4261 tggcaggctg ctcttgataa tctcctccag cgttagaacc gggcagcaac tgcggagggt
     4321 gtacaggttc aggcccacgg gcggtgtgac caccgccagt tccaggttga ttaccaggac
     4381 gatcccgtac cagaggggat cgtagcccat ggctttgatc aggggaagaa ggatgggggt
     4441 cgtgatcacc atcaaagaac cggcatccac aaacatcccg cctataatca aaataccctg
     4501 gatgagcagg accatgacca gcttgggcat gttcaggccc atgaagaaac tggtcaccat
     4561 ttcggggacc cgaaccatgt tcaggtagtc tccgaagatc ttggcgcagc ccatgatcag
     4621 caggatggcc acactgatca tcatgctttc ccggatgatc tggacgatcc gccggaaatt
     4681 catgttgcgg tagaccaggc cggcgatgag aaaggccccg atggcgccca gagccgaagc
     4741 ctccgtgggt gtggccaccc ccagatagat ggaaccgagc acgaggaaaa tcaggatgcc
     4801 cgtatgccag accctcgcca gactagtcca gcgttcccgc caggagaatt tgagctcggc
     4861 cgaacggggg gccagttcgg gagtgatcag aacgcggaag aagacgtaga tactgagcat
     4921 ggcggccagg accaggccgg gcacgatccc cccgatgaag agcttagcca cggattcgcc
     4981 ggtgatcgcg ctgtagataa tgaaggggac gctgggaggg atgagcatgg ccagggcccc
     5041 ggccgtgacc acggagcccg cggcgatttt ggtggaatat cccctcttca gcatctccgg
     5101 gacggccatg gacccgattg cggccacgcc ggccacactg accccgctca ctgcgccgaa
     5161 gaccgtgcag gcgccgatcg aagcgatggc caaccccccg g
//
LOCUS       DASPRR010000576         5103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1758398, whole genome shotgun sequence.
ACCESSION   DASPRR010000576 DASPRR010000000
VERSION     DASPRR010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758398"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1329
                     /locus_tag="VEL68_21190"
     CDS             400..1329
                     /locus_tag="VEL68_21190"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXZ38558.1"
                     /translation="MEVIWKAKDFGRSFKNPVLTLGNFDGVHLGHQRIFEQVRRKAKE
                     ISGEAIAYTFAPHPVQLLKPGQEPFLLLPIAERIRLIEEAGIDVTICAPFTPEFASLT
                     AEEFVRDILHRQIRVRQVYVGQNTSFGRGRTGSVSLLKEFGKRYGFVVEAMEAVEVKG
                     TLISSSRVRRLIRQGDMPGATELLGRPPLLVGEVIHGFGRGSKKLGFPTANLKIDEVL
                     VPRPGIYAVWVVYEGQRLPGVANLGWNPTFQDRKFSIEVYILNFDKDIYGQPLRVEFV
                     ERQRDEVTFRGPEELIAQIKKDVEQAKRILGID"
     gene            1504..2505
                     /locus_tag="VEL68_21195"
     CDS             1504..2505
                     /locus_tag="VEL68_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ38559.1"
                     /translation="MPLVVEAHRKSRSAYHLPEAAPKADQGIPCNLCVNECRIPEGGL
                     GYCGLRRNQGGKLAGVSAEEGKLSWYYDPLPTNCVADWVCPGGTGAGFPQYAHCTGPE
                     RGYKNLAVFFHACTFNCLYCQNWHFREETLKARTIPVSRLVSDVDERTSCICYFGGDP
                     AAQLPFSLTASRKALAAKQKRILRICWETNGSMHPGLLDEMIQIALESGGCIKFDLKA
                     WDESLHKVLTGTTNRRTLENFRRAGERSRTRPVPPPLIASTLLVPGYVEDEEVRSLAR
                     FIASVNPEIPYSLLAFHPHFYMSDLPFILRSTAERCSQVAREEGLENVRLGNIGLLR"
     gene            2643..3332
                     /gene="radC"
                     /locus_tag="VEL68_21200"
     CDS             2643..3332
                     /gene="radC"
                     /locus_tag="VEL68_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXZ38560.1"
                     /translation="MDDKQGYLGHRKRLRDRFHKGGPDGLQDYELLELLLTYAIPRKD
                     VKPYAKALIKRFGSLAGVLDASQKEIEEVEKIGPISSTLIRLIKEICGAYLAEKIKGR
                     EVLSSPQAVLDFARMKLSGLPHEAFMVIFLDSKNRVLDFKVLLEGTVDRAVIYPRRIV
                     EEALSRHAAGIIFVHNHPSGDAQPSPEDKNLTRSLMEAARTIDLRVLDHLIVGKEGYC
                     SFVESRLMPGS"
     gene            complement(3550..4635)
                     /locus_tag="VEL68_21205"
     CDS             complement(3550..4635)
                     /locus_tag="VEL68_21205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXZ38561.1"
                     /translation="MGTLSNYPRGIILALVFLFLMANLAFGETKVFVEEYTYQASEVD
                     SKLSSGVIALEQVKRLLLEKLGTYLESETEVKNFHLSKDQIVILTAGIVSTEIIDEKW
                     DGKTYSLKAKITADPKDAAKAINKLRQDRQKSKELEEARKKADEALGEVEKLKKEWAI
                     AKAEKLDLRGYKEAINRLSATDWVDRGYALVTSGKNQEAVEAFTRAIELDPKNANTYY
                     HRGGVYGRLEDYRRAIRDFDRAIELNPRLEEVYTDRGVAYGMLGDYQQAIREYDKAVE
                     LNPKDTRAYVNRGIAYEELKEHQQAIRNYNKAIELNPKDARAYLNRGFAYAELGDHQQ
                     ALEDYKTAAQFGSRTAQHYLSSKGISW"
     gene            complement(4696..>5103)
                     /locus_tag="VEL68_21210"
     CDS             complement(4696..>5103)
                     /locus_tag="VEL68_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ38562.1"
                     /translation="LSDRLGRGRVMLVLAATSMACSFLFGWSIGWPLALVMGIGMIYA
                     FSALGDSPIFSAALTEVVDTAYLGSAFGLRSLMGFGAGAVAPLAFGAVLDWSNALPAG
                     QRLYANWGWAFGILGLGGLGAVWATRQFGKVHK"
BASE COUNT         1152 a         1412 c         1309 g         1230 t
ORIGIN      
        1 gttccggttg ggggaccggg tgcgcgtgcg cgtagaggcc gccagcctgg agcgccgcca
       61 ggttgatttc ggcctggtcc aatgataaaa aatatttatc cgcagattac gcagatttcg
      121 cagagagtgc gaaggcatcg attgctatac catttacctt caagcttatt aatggaggcg
      181 cattttattt tttgacgtta tgtagggcct gatccccgat caggcctaaa gtatcggtgc
      241 gttcggggaa cgcaccctac atttagggca aattcattta atgcgtttgt attaaattct
      301 gttattactt ctcgactttt ttacttaagg cacttgaggc acctaagtgc acttaagcac
      361 acttttagtt ggtcacggct ctcctgcgaa aattaaatta tggaagtcat ctggaaagcg
      421 aaagatttcg ggcggtcctt caaaaacccg gtgctcactc tcggaaattt cgacggggtg
      481 caccttggtc accagcgcat tttcgagcag gttcgccgaa aagctaaaga gatcagtgga
      541 gaggctatcg cctatacctt tgctccccac cctgtgcagc ttctgaagcc ggggcaagaa
      601 ccgtttctcc ttctgcccat agcagaaaga atccggctga tcgaggaggc gggaatagac
      661 gtgaccatct gtgccccatt cacccccgaa tttgcgtctc tgaccgccga ggaattcgtg
      721 agggacatcc tgcatcgaca gattagagtc cgccaggttt acgtgggcca gaatacctct
      781 ttcggccggg gccgcacagg aagcgtctcc ctgctgaagg aattcgggaa gcgctacggc
      841 tttgtcgttg aggccatgga agcggtggag gtaaagggaa ccctgatttc cagcagtcgg
      901 gtccgcaggc tcattcgcca gggggatatg cccggggcta cggaactctt ggggcgtccc
      961 ccgctcctgg ttggagaggt catccacggc tttggacggg gcagcaagaa gctggggttc
     1021 cccacggcga atctgaagat cgatgaagtg ctcgttcccc gaccgggaat ctacgcagtt
     1081 tgggtcgttt acgagggtca aaggttgcca ggagtggcca acctaggctg gaaccccacc
     1141 ttccaggatc ggaaattttc tatcgaggtt tatatcctca acttcgacaa agacatctat
     1201 gggcagcctt tgcgggtgga attcgtggaa cgccagcgcg acgaagtcac tttccggggc
     1261 cccgaagagc tgatcgccca gatcaaaaag gacgttgagc aggccaagag aatcttaggg
     1321 atcgattgaa tctcatttcc aacagaagcc tcctttatat atttccccct ttataaaagg
     1381 ggggcggggg ggatttctga gggaaccatg ccccgctgcc gcctctgtaa cctctcctca
     1441 aatccggttt ccaaagaaat cggtgtctgc gcgaattgtt tgaaatcgga acccgatgag
     1501 gccatgcctc tcgtcgtgga ggcccatcgg aaaagcagat ccgcctatca cctacccgaa
     1561 gcagccccga aagccgatca ggggattccc tgtaatctct gcgtaaatga atgccggatt
     1621 cccgaaggcg gattggggta ttgcgggttg aggcggaacc agggaggaaa actcgccggg
     1681 gtttcagccg aagagggaaa gctctcctgg tattacgatc ccctgcccac caactgcgtg
     1741 gccgactggg tatgccccgg cggcacaggg gccgggttcc cccaatatgc ccattgcaca
     1801 ggcccggaaa gagggtacaa aaacctggcc gttttctttc acgcctgcac cttcaactgc
     1861 ctctactgcc aaaactggca cttccgcgag gagacactca aggcccgcac gattcctgtg
     1921 agccgcttgg tttccgatgt ggatgagaga acctcctgca tctgctattt cggaggggac
     1981 ccggcggccc agctcccctt ttccctgacc gcctcacgga aggctttggc ggcgaagcaa
     2041 aaaagaatcc tccgcatctg ctgggagacc aacgggtcca tgcatcccgg cttgctcgac
     2101 gagatgatcc agatcgctct ggaatcgggc ggttgtatca aatttgattt gaaggcgtgg
     2161 gacgagagcc tgcataaggt tcttacgggg acaaccaacc gaaggacgct ggagaatttc
     2221 cggagggcag gagagagatc caggacaagg cccgttcctc ccccgctcat tgccagcacg
     2281 ctcctggtgc ccggctatgt ggaagatgaa gaagtgaggt cactcgcccg gttcatcgcc
     2341 tccgtcaacc ccgaaatccc ctacagcctc ctggcctttc acccccattt ttacatgtcc
     2401 gatttgcctt ttatcctgag atcaaccgcc gaaagatgct cccaggttgc ccgggaagag
     2461 gggcttgaaa atgtgagatt gggcaacatt ggcttacttc gatagccttg ttgaaagagt
     2521 gtatgagacc cgagaagaat ttgctttttt tgaaaaattc cataaaatga gcctcatact
     2581 aggcaatctt cttctttatt tttcatcaac cttgcctttg atgattgaag ggggacggaa
     2641 gcatggatga caagcagggt tatctcggcc accggaagag gttgagggat cgattccata
     2701 aggggggacc cgacgggctg caagactatg aattgctgga gctcctcctg acctatgcca
     2761 tcccccgaaa ggatgtcaag ccctacgcca aagctttgat caagcgcttc gggagccttg
     2821 ccggggtcct ggatgcgagc cagaaagaaa tagaggaggt ggagaagatc ggccccatct
     2881 cttccactct catccgcctg atcaaagaaa tctgcggtgc ataccttgcc gagaagatca
     2941 aaggaagaga ggtcctctca tcgccacagg cggtcctgga tttcgcccgg atgaaattgt
     3001 ccggcctccc ccatgaagcc ttcatggtca tttttttgga ttccaagaac cgggtcctcg
     3061 atttcaaggt cctgctggaa ggaactgtgg atcgggcggt catctatccg cgccggattg
     3121 tagaagaagc cctttcccgc catgccgccg ggatcatctt cgtccacaac caccccagcg
     3181 gcgacgccca gccgtccccg gaggataaga acctcacccg gtccctgatg gaggccgccc
     3241 gtaccatcga cctccgggtc ctcgatcatt taatcgtcgg gaaggaaggg tattgcagct
     3301 tcgtcgagtc acgcctgatg ccgggttctt aggggaaaag gctttcgaga tttgctcggg
     3361 aggcagagaa ttccgctaga gtctaggctc ttggataaga tgtggataaa atgcagggtg
     3421 ggtgggttcc ccgaatggac cgatgtactt cccgatatgt cacaatattt caaaaattcg
     3481 ccatagagaa ataagaaagg cagaatttga taaccctgcc tttcaacaca cccaaggact
     3541 ttgagaaaat taccaactaa taccctttga gcttaaataa tgctgtgccg ttctagaccc
     3601 gaattgggca gccgttttat aatcctcaag ggcctgctgg tggtctccaa gttctgcata
     3661 ggcaaatcca cggttaagat aggcccttgc atctttcggg ttcagctcga tggccttgtt
     3721 ataattcctg attgcctgtt ggtgctcttt aagttcttcg taggcaattc cgcggttaac
     3781 ataggccctt gtatccttcg ggttcagctc gacggccttg tcatactctc tgattgcctg
     3841 ctgatagtcc ccaagcatcc cgtaggcaac tccacggtcc gtatacacct cctctagcct
     3901 cggattcagc tcaatcgccc tgtcgaaatc ccggattgcc cgccggtagt cctccagtct
     3961 tccgtagaca cctccgcggt ggtaataagt atttgcattt ttcgggtcca gttcgatggc
     4021 cctggtaaat gcctccaccg cttcttggtt ctttccagag gttactaatg catatcccct
     4081 atccacccag tctgtagcac ttaatctatt tattgcttcc ttgtatcccc tcaaatccaa
     4141 tttctctgct ttcgctatgg cccattcttt ttttagcttt tcaacctctc ctaacgcctc
     4201 atctgctttc tttctcgctt cctccagctc ttttgacttc tggcggtctt ggcgcaattt
     4261 gtttatcgct ttggccgcat cctttgggtc tgcggttatc ttcgccttca aagaataagt
     4321 cttcccatcc cacttttcat caataatctc agtgctgact atcccggcgg tgaggataac
     4381 aatctggtct ttgcttaggt ggaaattttt gacttcggtt tcgctttcga ggtaagtccc
     4441 caatttctct aaaaggagtc tcttgacttg ttccaaggcg attactccac tcgatagttt
     4501 gctatctact tcacttgcct gataggtata ttcttcgaca aagacttttg tttccccaaa
     4561 agccaaattt gccataagaa aaaggaacac cagggcaagg atgatccccc tgggatagtt
     4621 actcagagtc cccatttcta tgcctccgat gtccgtgtct catccgcata atcgaaaccc
     4681 gacaataagc tttgcttact tgtggacctt gccgaattgg cgagtggccc acaccgcgcc
     4741 cagccctccc agccctagta tgccgaaagc ccatccccaa ttggcgtata atcgctgccc
     4801 agcaggcagg gcgttgctcc agtccaggac tgctccgaaa gccaaaggtg ccacggctcc
     4861 ggcaccgaac cccattaggg acctgaggcc gaaagcactt cccagatagg cagtgtctac
     4921 cacctcggtc aaggccgctg agaaaatggg agagtctccc agcgccgaaa acgcatagat
     4981 catcccaatc cccatgacca gcgccagagg ccacccgatg gaccacccga agaggaagga
     5041 gcaggccatg ctggtagccg ccagaaccag catcacccgg cctcggccca gccggtccga
     5101 tag
//
LOCUS       DASPRR010000577         3675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1588444, whole genome shotgun sequence.
ACCESSION   DASPRR010000577 DASPRR010000000
VERSION     DASPRR010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588444"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="VEL68_21215"
     CDS             <1..1714
                     /locus_tag="VEL68_21215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ38563.1"
                     /translation="PSPAGIPGEKLLGFTLSLKHGGFLMKEWTEGSDREAIRRRRLRI
                     IIPAFPAFNIYSRVARVTTALGPVTVATAAREMEGWDVEVIDENNYQRSGPVTADGLP
                     DHGALQQLRPADLVGLYGGLTSTIPRLYELARFYRERSVPTIAGGQHFVDENIGEALQ
                     NGVDVVVRGEGEETVKELLQAFEGRLSREKIAGIAYLDGDRLVLTPERTPLSEFDSFP
                     LPDFSLVRYARIRLYPVGRVRGCGMDCEFCTVKGKPRYARVERLMEQFASLYETRGAK
                     HFFIVDDLFGQDRRETFRLCQLLRDYQRRIKTRFKITVQIRLDKGKDAELLQSMREAG
                     VQNVAIGFESPIGEELKAMDKRLRPEDMIDLSHRFHKAGFFIHGMFIFGYPAAEGIEF
                     SMPASERIQRFRKFIRNARLDSVQVLLPAPLPGTELTHRLRKQNRIFPKEWVGWEYYD
                     GNFPLFIPDAPMTPEEMQTSSHRIMGRFYRFKHMFHIGLHILWFPILFFYLPNLQRGW
                     EKWHRAWANQVTRFGGWLIMRRWFAAFRKDPFSRKLAEAKRILSRSLPKPGHPSLSVK
                     RQNQ"
     gene            complement(1725..2747)
                     /locus_tag="VEL68_21220"
     CDS             complement(1725..2747)
                     /locus_tag="VEL68_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXZ38564.1"
                     /translation="MGNGILNPSNSPPLLRVQELSKYFALSVPWLSRVFGRQSPPILR
                     AVDGVSFEIQRGKTFALVGESGCGKSTVARVIVGLYHPTAGDIYFQGKSLEDPSVKSS
                     PESHTRMQMIFQDPYASLDPRWRVRDIIAEPIRAFHLARNRSALGERVSELLLQVGLS
                     PSDGEKYPHEFSGGQRQRISIARALSSHPDFLVCDEPTSALDVSVQAQILNLMKELQG
                     RLGLTYLFISHNLAVVNHISDHVGVMYLGRLCEVAPKRTLFNQPQHPYTRLLLDTIPD
                     LDLSGRRRQPVTGEVPNPIHPPSGCAFHPRCPSAEARCRTEKPVPRPSKDSTVACHAV
                     EEGRLQ"
     gene            complement(2740..>3675)
                     /locus_tag="VEL68_21225"
     CDS             complement(2740..>3675)
                     /locus_tag="VEL68_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38565.1"
                     /translation="RRGVLVAVDDVSFSIEEGEVLGVVGESGAGKSVTGAGVIGLLEP
                     PGRIARGNIRFEGRQLNGLPQEAMRRIRGAKIGAIFQDPLTSLNPLYSIGRQIIETLT
                     THTDLSPSQAWSRAIDLLNEVGIPAAERRVDSYPHEFSGGMRQRVVIALAICANPRLI
                     IADEPTTALDVSIQAQIIALLRRLCHEHRTAIMLITHDMGVIAEMSDRVAVMYAGRIA
                     EIGALRDVVKSPKHPYTAGLMDSIPKVGQGKDQLSHIRGAMPRLQEIPPGCSFHPRCP
                     EAFDRCQVERPDLMDTGHPKQIASCWKFAGKTWNG"
BASE COUNT          754 a         1081 c         1124 g          716 t
ORIGIN      
        1 cccgagtccg gcggggattc cgggggagaa gcttctaggt tttactctct ctttaaagca
       61 cgggggattt ctcatgaaag agtggaccga gggctcggat cgagaggcca tcagaaggcg
      121 caggctgagg atcatcatcc cggcctttcc cgcattcaac atctattccc gggtcgcccg
      181 ggtcactacc gctctgggac cggtgaccgt ggccactgct gcccgggaga tggaagggtg
      241 ggacgtcgag gtcatcgatg agaataacta ccagaggtcc ggcccggtaa ccgctgatgg
      301 cctgccagac catggagccc tccagcaatt gagacccgct gacctggtcg gcctttacgg
      361 agggttgacc agcaccattc cccgcttgta cgagctggcc cggttttacc gggagcggtc
      421 ggtacccacc atcgccggcg ggcagcactt cgtcgatgaa aacatcggcg aggccctgca
      481 aaacggagtt gacgtcgtgg tcagaggaga gggggaagag acggtcaagg aactcctgca
      541 ggctttcgaa ggccggcttt ccagggagaa gatcgcgggc atcgcctacc tggatggaga
      601 ccgtttggtc ctgactccgg aaagaacgcc cctgtcagaa ttcgattcct tcccgcttcc
      661 cgatttttct ttggtccgtt atgcccggat ccggctctat cctgtcgggc gggtgcgggg
      721 atgtggaatg gattgtgagt tctgcacggt caaagggaaa ccccgctatg cccgggtgga
      781 gcggctgatg gagcagttcg cctctctcta cgaaacccgc ggcgctaagc acttcttcat
      841 tgtggatgac cttttcggcc aggaccggag ggagacgttc cggctctgcc agcttctccg
      901 ggactatcag aggaggatca aaaccaggtt taaaattacc gttcagatcc gtctggacaa
      961 aggcaaagac gcggaactcc tccaaagcat gcgcgaagcc ggggtccaga acgtagccat
     1021 cggttttgaa tcccccatcg gggaagaatt aaaggccatg gacaaacggc tcaggcccga
     1081 agacatgatc gacctttccc acaggttcca caaggccggt ttttttatcc acggaatgtt
     1141 catttttggg tatcccgcag cagagggaat cgagttttcc atgccggcct cggaaaggat
     1201 ccaaagattt cgaaagttca ttcgcaatgc gcggctggat tcggtccagg ttttgctacc
     1261 tgccccgctg ccggggacag agttgaccca ccgactgagg aaacagaaca gaatcttccc
     1321 caaggaatgg gtggggtggg agtactacga cgggaatttt cccctattca tccccgatgc
     1381 gcctatgacc ccggaagaaa tgcagacctc ctcccaccgg atcatgggac gattctaccg
     1441 cttcaagcat atgttccaca tcgggctgca catcctctgg tttcccattc ttttctttta
     1501 tctccccaat ctgcaacggg gctgggaaaa atggcaccgg gcctgggcaa atcaagtgac
     1561 ccggttcggc ggctggctga tcatgcgccg atggtttgcc gcattcagga aagacccttt
     1621 ctcccgcaag ctggccgagg ccaaaagaat cctctcccgc tctctgccga aacctggcca
     1681 tcccagtctt agcgtaaaga gacaaaatca ataaagggtc cccattactg cagacgccct
     1741 tcttcaacgg cgtggcaggc cacagtactg tctttcgagg gtctaggcac gggcttctcc
     1801 gtccggcatc gggcctccgc cgatggacag cggggatgga aagcgcaccc ggagggggga
     1861 tggatggggt tgggcacttc cccggtcacc ggctggcgcc tgcgcccgga gaggtcgaga
     1921 tcgggaatgg tgtcgaggag cagccgcgta tagggatgct gcggctggtt gaagagggtt
     1981 cgcttggggg ccacctcgca gagccggccc aggtacatga cccctacatg atccgagata
     2041 tgattcacca ccgccaggtt atggctgatg aataaatagg tcagtcccag gcggccttgg
     2101 agctctttca tcaggttcag gatctgggcc tggactgaaa catccaaggc cgaagtgggc
     2161 tcgtcgcaga ccagaaaatc gggatggctg gagagggccc gggcgatgga aatacgctga
     2221 cgctgccccc cgctgaactc atgggggtat ttttccccgt cgctggggga gagacccacc
     2281 tgcagaagca gctcggagac ccgttccccc agcgcggatc gattcctcgc cagatgaaag
     2341 gcccggatcg gctcggcgat gatgtcgcgg acccgccagc gcggatccag actggcatag
     2401 ggatcctgga agatcatctg catccgggtg tgagactccg gcgaggactt taccgaagga
     2461 tcctccaaac tctttccctg gaaataaata tccccggctg tggggtgata aagacccacg
     2521 atcacccggg ctacggtcga tttcccgcac cccgactccc ccaccagggc gaaggtcttt
     2581 ccccgctgga tttcaaagct gaccccgtca acggctctca ggatcggggg ggactggcgg
     2641 ccgaagaccc ggctgagcca ggggaccgag agagcaaaat acttcgagag ttcctgcacc
     2701 cgcaggagcg gaggggaatt tgaggggttc aagatgccgt tacccattcc aggtcttccc
     2761 cgcaaatttc cagcaggagg cgatctgttt tggatggccg gtatccatca aatcgggtcg
     2821 ctccacctga cagcgatcga aggcctccgg acagcgggga tggaaagaac agcccggcgg
     2881 aatttcctga agccggggca tggccccgcg gatgtgcgag agctgatctt ttccctgtcc
     2941 gaccttgggg atggagtcca tgaggcccgc ggtgtaaggg tgcttggggc tcttcactac
     3001 gtcccgaaga gcaccaatct ctgcaatccg gccggcgtac atgaccgcca cccggtcgga
     3061 catctcggcg atgaccccca tatcgtgggt aatcagcatg atcgccgtgc ggtgctcgtg
     3121 gcacaggcgc cgcaggaggg cgatgatctg cgcctggatc gacacgtcaa gagccgttgt
     3181 cggttcgtcg gctatgatga gccgggggtt agcgcagatg gccaaggcga tcaccacccg
     3241 ctgtcgcatt cccccggaaa actcgtgggg gtaagagtcc acccggcgct ccgcggcagg
     3301 gattcccacc tcattcaata aatcgatggc ccgggaccac gcctgagagg gagaaaggtc
     3361 cgtgtgggtg gtgagggttt cgataatctg gcggccgatg gagtaaagtg ggttcagact
     3421 ggtcaggggg tcttgaaaaa tcgccccgat cttcgcgccc cggatccggc gcatcgcctc
     3481 ctggggcaag ccgttgagct gccgcccttc gaaccggatg ttgccccttg cgatccgccc
     3541 cggcggctcc aggaggccga tgaccccggc gccggtgacc gatttccccg cgcccgattc
     3601 gcccacgact cccaggactt ctccctcctc tatggagaaa gagacgtcat ccacggccac
     3661 cagaactccg cgccg
//
LOCUS       DASPRR010000578         5411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1269794, whole genome shotgun sequence.
ACCESSION   DASPRR010000578 DASPRR010000000
VERSION     DASPRR010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269794"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VEL68_21230"
     CDS             complement(<1..205)
                     /locus_tag="VEL68_21230"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HXZ38566.1"
                     /translation="MFLQKILEHKKQEMLERKALLPLSELKRRLEEEPTPLDFERAIR
                     SRPEALIAEVKRSSPSRGRIREDF"
     gene            complement(397..1407)
                     /gene="trpD"
                     /locus_tag="VEL68_21235"
     CDS             complement(397..1407)
                     /gene="trpD"
                     /locus_tag="VEL68_21235"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXZ38567.1"
                     /translation="MIQEALAKVVQKTDLQESEMAQAMEEIMEGKVTPAQLGAFLTAL
                     RMKGETVEEVTGAARIMRQKATRIDVRSPVIVDTCGTGGDGQKTFNISTTTAFVVAAA
                     GLTVAKHGNRAVSSRCGSADVLEALGVRIDVSPDVVEECLHQIGIGFLFAPKLHGAMK
                     YTAGPRREIGLRTIFNMLGPLTNPASATSQLIGVYDPKLTEMFAGVLRNLGTRRAFVV
                     HGSDGLDEATVTGETRVSELKEGRIITYNIHPMEFFGEAFGPEELNGGDASTNAAITR
                     AILAGKDGACRKTVLLNAALAIVAGEKAETIQEGIRVAEDCLDSGIAGKKLEALVELT
                     HR"
     gene            complement(1404..2000)
                     /locus_tag="VEL68_21240"
     CDS             complement(1404..2000)
                     /locus_tag="VEL68_21240"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HXZ38568.1"
                     /translation="MVLMIDNYDSFTYNLVQYLGQLGAEVAVHRNDEVSIGEIERMDP
                     EAIFLSPGPCTPQKAGITVEVIRHFHKTLPILGVCLGHQAIGYAFGAEVVRADRLMHG
                     KTSPVFNDGRTIFQGLPNPFPAGRYHSLLVRRETLPSFLEISAETKEGEIMALRHCDY
                     PLEGVQFHPESVLTPSGKRILRNFLELNLRDKERSLER"
     gene            complement(2015..3523)
                     /gene="trpE"
                     /locus_tag="VEL68_21245"
     CDS             complement(2015..3523)
                     /gene="trpE"
                     /locus_tag="VEL68_21245"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HXZ38569.1"
                     /translation="MYCPSFDEFKTLAQKGNLVPVYREILADEETPVTALMEISRRPY
                     AFLLESVEGGEKWGRYTFLGADPQAVFRIRAGEVEVREDGGVQRVRPEGNPLACLKDL
                     MGRYRPVFPAGLPRFFGGAVGYLSYDMVRHFEKKLTRPPANAPEMDDAVFLLTDSLLV
                     FDNVRHTIKIVLCVRIPEGSLDLEALYRKSTERIEGIIDGLRRHAARSARTFIPGKNN
                     PSFQANMTDEAFKDIVRRAKEYIQAGDIIQVVLSQRFRGETCLDPVDLYRALRFVNPS
                     PYLFFLKLEDSYLIGSSPEVMVRLEDRIAELRPIAGTRRRGRTEQEDRSFADELLNDP
                     KERAEHVMLVDLGRNDLGRIARVGSVQVNQYMTVERYSHVMHLVSNVHAQLAEGRDCF
                     DVLKATFPAGTLSGAPKVRAMEIIDELEPCCRGPYGGAMGYFSFSGNMDFGITIRTLV
                     LQGNRLSLQVGAGIVADSNPDREYEETLNKAAGLMKAVRLAASGFEIMEEEP"
     gene            complement(3792..4640)
                     /locus_tag="VEL68_21250"
     CDS             complement(3792..4640)
                     /locus_tag="VEL68_21250"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HXZ38570.1"
                     /translation="MNGIESTEKFKVGIIGGTGGMGRWFARFFTQEGHPVFVSGRSRG
                     PSLSEMAGACSVVIVSVPIGVTCEVIRKVGPFMKEDSLLMDLTSLKAEPMRAMLESSV
                     SEVIGLHPLFGPSQPSLAGQNIALCPGRGKNWLPRVRNLLGKKGARMVETTPERHDEI
                     MATVQGINHLDTIILGLFLQEVGLDPAILERFSTPILRTKMEIIQKVFSHPALYAEII
                     ALNPYLRRMIDLYQRTFAEMKIVTEKGNAEDLKRLIEQRKTGPWASATPAGGSFSVRG
                     TPLAKP"
     gene            complement(4680..>5411)
                     /gene="aroC"
                     /locus_tag="VEL68_21255"
     CDS             complement(4680..>5411)
                     /gene="aroC"
                     /locus_tag="VEL68_21255"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXZ38571.1"
                     /translation="DWTYFKKYGIRDHRGGGRASGRETAARVAAGAVARKVIARAGIE
                     VFAYTLELGGIAVDGFSRTEISRNRFLCPDSRAAEKMGERIEAAMVEGDSLGGVVEVR
                     VLGCPPGLGEPVFDKIDADLAKALMSLGTVKGVEIGDGFEAARMKGSTSNDPIAPGGF
                     MANHAGGILAGITNGEEIVIRAACKPIPSIRKEQDTIDIHGNPRRVSIPGRHDVCVIP
                     RIIPVCEAMVCMVLADHLLRQKSIS"
BASE COUNT         1080 a         1636 c         1522 g         1173 t
ORIGIN      
        1 cgaagtcctc ccggattctc cctctggagg gggagcttct cttcacctcg gctattagcg
       61 cctctgggcg ggagcggatg gccctctcaa agtcaagagg ggtaggctcc tcctccaggc
      121 gtcgcttcag ctcggaaaga gggaggaggg ccttgcgctc caacatttcc tgttttttat
      181 gctccagtat tttttgcaga aacatgcttt cctcaactgt ggtgattctg taaaaagtcg
      241 aaattcaaag aatctgtcat accggcgaaa gccggaatcc aggcttaccg gtgaaaaccg
      301 ggacccaatc tttgagatgg ttcccaactt tcgtcgggac gacatctgga ccccggcctt
      361 cgccggggtg acggcgcaag acttcttacg aaatcatcaa cgatgggtaa gctcgacgag
      421 ggcctcgagc ttttttccgg caatgccgct gtcgagacaa tcctctgcaa cccggatccc
      481 ttcctgaata gtctcagcct tctccccggc cacgatggcc agggcggcat tcagaagaac
      541 ggttttccgg caggccccgt ctttcccggc gaggatcgcc cgggtgatgg cggcgttggt
      601 ggaggcgtct ccgccgttca gttcttcggg tccgaaggcc tcgccgaaaa attccatggg
      661 gtggatgttg taggtgatga tccttccttc tttcagttcc gagaccctcg tttctccggt
      721 caccgtggcc tcatccaatc cgtcagaccc atggacgaca aaggctctcc gggtccccaa
      781 attccggagg accccagcaa acatttcggt gagcttcggg tcgtaaactc caatgagctg
      841 cgacgtggct gaagcgggat tggtcagggg ccccagcatg ttaaagatgg tcctcaggcc
      901 gatctcccgg cgcggtccgg cggtgtattt catggccccg tgcagcttgg gggcgaagag
      961 gaagccgatt ccgatctggt gcaggcattc ctcgactaca tccggagaaa catcgattcg
     1021 gacccccagc gcctcgagga catcggcgct tccgcacctg ctggagactg cgcgattgcc
     1081 atgcttggcc acggtcaacc ccgctgcggc cacgacaaag gccgtggtag tggagatgtt
     1141 aaaagtcttc tgcccgtctc ctccggtgcc gcaggtatcc acgatgactg gggatcggac
     1201 atcgatacgg gtggcctttt gcctcatgat tcgagcggcg ccggtcacct cctccaccgt
     1261 ttctcccttc atccgcaggg cggtaagaaa ggcaccgagc tgggccggcg tgaccttccc
     1321 ctccatgatc tcctccatgg cctgagccat ttcgctttcc tgaaggtcgg ttttttgaac
     1381 gaccttggcc agagcttcct gaatcatctc tctaggctcc tttctttatc ccgcaggttg
     1441 agctccagga aatttctcaa gattctcttc ccgctcgggg tgagcaccga ttcgggatga
     1501 aactgaaccc cttccagcgg atagtcgcaa tgacggaggg ccatgatctc tccttctttg
     1561 gtttctgcgc tgatctccag gaaggaggga agcgtctctc tcctgaccag gagggaatga
     1621 taacgcccgg ccgggaaggg attggggagg ccctggaaga tcgtccgccc gtcattaaaa
     1681 accggagagg tcttgccgtg catcaggcgg tcggcccgga ccacttcggc gccaaaggca
     1741 taaccgatgg cctgatgccc gaggcagacc cccaggatgg gaagggtctt atggaaatgc
     1801 cggatcacct ccacggtgat gccggctttt tggggtgtac aaggcccggg ggagagaaaa
     1861 atggcttccg ggtccatcct ttcgatctcc ccgatggaaa cttcatcatt ccggtggacg
     1921 gccacctctg ccccgagctg gcccaagtat tgaaccaggt tgtaggtgaa ggaatcgtaa
     1981 ttgtcgatca ttaagaccat atcctttctc ctttctatgg ctcttcttcc atgatttcaa
     2041 accccgaagc ggccaggcgg actgccttca tcaatcccgc agccttgttc aaagtctcct
     2101 cgtattcccg gtccgggttg gagtcggcca cgatgccggc acccacctgc agcgataggc
     2161 gattcccctg cagcaccagg gtccggatgg taattccgaa atccatgttc cccgaaaagc
     2221 tgaagtaacc catggccccg ccgtaggggc cccgacagca aggctccagc tcgtcgatga
     2281 tctccatggc ccgcaccttg ggtgcgcccg agagcgtacc ggcggggaag gtggccttca
     2341 ggacatcgaa gcagtcccgg ccttcggcaa gctgggcgtg cacgttggag accagatgca
     2401 tcacgtggga ataccgctcc acggtcatat attggttgac ctggacgctg cccacccggg
     2461 cgatccttcc caagtcgttg cgtcccaggt caacgagcat gacatgctct gccctctctt
     2521 tgggatcgtt gaggagttcg tcggcgaatg atcggtcttc ctgttcggtc cttccccttc
     2581 ttctagtgcc ggcgatggga cggagctccg cgatccggtc ttcgagacgg accatgactt
     2641 cgggggaaga gccgatgaga taagagtcct ccagcttgag gaaaaagagg taaggggaag
     2701 ggttgacaaa gcgtaaggcc cggtagaggt cgaccgggtc caggcaggtt tcaccccgga
     2761 agcgctggga gaggacgacc tgaataatgt ccccagcctg gatatattct ttggcccgcc
     2821 ggacaatgtc cttgaaagcc tcgtcggtca tgttggcctg gaaggaaggg ttattttttc
     2881 cgggaatgaa ggttctggct gagcgggcag catgtcgccg caaaccgtcg atgatcccct
     2941 cgatcctttc cgtcgatttc cggtaaaggg cctccagatc aagtgaccct tcggggattc
     3001 ggacgcagag cacgatctta atcgtatgcc gtacattgtc gaataccagg aggctgtcgg
     3061 tgaggagaaa gacagcgtcg tccatctccg gggcatttgc cggaggccgg gtcaattttt
     3121 tttcaaaatg acgcaccatg tcgtaggaaa gataccccac ggccccgccg aaaaaacgcg
     3181 ggagtccggc cgggaataca gggcggtacc ggcccatgag gtccttcagg caggcaagcg
     3241 gatttccctc cggccttacc cgctggacgc ctccatcttc ccggacctca acctccccgg
     3301 cccttatccg gaagacggcc tgcgggtcgg cgcccagaaa ggtatatcgc ccccacttct
     3361 ctcccccttc cacgctttcc agcagaaaag catagggccg gcggctgatc tccataaggg
     3421 cggtgaccgg ggtttcctca tcggccagaa tctcccgata cacgggaacc agatttccct
     3481 tttgggcaag tgttttgaat tcgtcgaaag atgggcagta cataaatcct cctgaaacaa
     3541 aaaagccgtg tcttggggca cggccttcaa aatggaatga aagaatgagc cggggagccc
     3601 cttcaaggct ccccatggcc cagtttccgg atgcagcctc atcgaccggg cagcgggtaa
     3661 gccttgcgta tgtagcctcg ccagcaccag ttagaaatcg gcgatcctgg gttgatcatt
     3721 ctcttctctc ctggataaat acctgcccaa tgcattggtg gttcactttc tttttcctat
     3781 aaagaaactt gctacggttt tgccaagggg gttcccctga cggaaaaaga gcctcccgcc
     3841 ggagtggccg aggcccaagg gccggtcttc ctctgttcaa tcagcctctt caggtcctcg
     3901 gcattccctt tctcggtcac aattttcatc tcggcgaatg tcctctggta caagtcgatc
     3961 atccgccgga ggtacggatt cagagcgatg atctccgcat aaagagccgg gtgggagaaa
     4021 accttttgga tgatttccat cttggtccgc aggatggggg tggagaacct ttcgaggatg
     4081 gccgggtcca agccaacctc ctgcaaaaag agtcccagga tgatcgtgtc caaatggttg
     4141 attccctgga ccgtagccat gatttcatca tgccgctccg gcgtcgtttc caccatgcgc
     4201 gccccttttt ttcccaaaag gttccgcacc cgcggcagcc aattttttcc ccggcccggg
     4261 cagagggcga tgttttgtcc ggcaagggaa ggctggctcg gcccgaagag aggatgaagg
     4321 ccgatgactt cggaaaccga tgattccaac atggcccgca tgggttccgc cttcaaagaa
     4381 gtcaggtcca tgagcaggga gtcttccttc atgaagggac ccaccttgcg gatgacttcg
     4441 caggtcaccc cgatgggtac gctcacgata accacggaac acgcgccggc catttcggag
     4501 agacttggcc cacggcttct cccggaaaca aaaaccgggt ggccctcctg ggtgaagaac
     4561 cgggcgaacc accggcccat tccgcccgtg ccgccgatga tgcccacctt gaatttctct
     4621 gtggattcaa tcccgttcat ttatcaccat tcttccaacc tcaaactgag ccgtgggcct
     4681 caggagatcg atttctgccg caacagatgg tcggccagca ccatgcaaac catcgcctcg
     4741 caaaccggaa tgatccgggg gataacgcag acgtcgtgcc gtccagggat ggagacccgg
     4801 cgcgggtttc cgtggatatc gatcgtgtcc tgttccttcc ggatggaggg gataggcttg
     4861 caggcggccc ggatcacgat ctcctctccg ttggtaatcc ccgctagaat cccccccgcg
     4921 tggttagcca tgaacccacc cggggcaatg gggtcgttgg acgtcgagcc tttcatcctg
     4981 gcggcctcaa atccgtcgcc gatttctact cctttgacgg tgcccaggct catgagcgcc
     5041 ttagccaggt cggcatcaat cttatcgaag acgggttccc ccaggcccgg aggacaaccc
     5101 aagacgcgga cctccacgac cccgcccaga gaatccccct cgaccatcgc tgcctcgatc
     5161 ctttctccca tcttttcagc cgcccgggaa tcggggcaga gaaatctgtt cctcgaaatc
     5221 tctgtccggg aaaagccatc gacggcgatc ccccccagtt ccaaggtgta agcgaagacc
     5281 tcgattcctg cccgggcgat gactttgcgg gccacggctc ccgcagccac gcgggcggcc
     5341 gtttcccgcc ctgaggcccg cccgccgccg cggtgatccc ggatgccgta tttcttgaag
     5401 taagtccagt c
//
LOCUS       DASPRR010000579         6487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_623090, whole genome shotgun sequence.
ACCESSION   DASPRR010000579 DASPRR010000000
VERSION     DASPRR010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623090"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..668
                     /locus_tag="VEL68_21260"
     CDS             360..668
                     /locus_tag="VEL68_21260"
                     /inference="COORDINATES: protein motif:HMM:NF019150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HXZ38572.1"
                     /translation="MAVLIAIAACFLLILYSYFVADTVQTIITDAQMTKVDGKYMIAT
                     EYRPFVNYDAWYRFKFDSGTIQNQAVRLRGKTVKIKKYGWRIPFFSEYENVVKIEEVK
                     "
     gene            786..1535
                     /locus_tag="VEL68_21265"
     CDS             786..1535
                     /locus_tag="VEL68_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXZ38573.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGKTFTKLIKEITVAARTGGGDPG
                     GNPRLRSAITAAKAENMPKDNIDRAIKKGTGELPGVSYEEATFEGYGPGGVAILVEIL
                     SDNRKRTVADIRHIFSKNAGNLGEAGCVSWMFGKKGLIVFEKEKVDEEKLMNVALDAG
                     AQDVRETDKEFEVVCDPSDFEKVKKALEQTQLKPLLAEITMVPQSTIKLAGKEAQQML
                     RLMESLEDSDDVQHVYANFDIPDAEMEQLSA"
     gene            1588..2151
                     /gene="ruvC"
                     /locus_tag="VEL68_21270"
     CDS             1588..2151
                     /gene="ruvC"
                     /locus_tag="VEL68_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXZ38574.1"
                     /translation="MRILGVDPGMVSTGFGLIEFEKQGSLLYRESGVLRPRGTSVPPR
                     LQQIFRGLTLLIETAKPRFMAVERPFFGKNVKSAMLLGQARGTALLAAAEAGLEVHEI
                     SPLEVKQAVVGYGRADKGQIQAMVRSILGIRQTLTADAADALAVALCLAHSLPLKTSL
                     QEALLKDSRGERRRSTRWTEKDLGDFR"
     gene            2148..2750
                     /gene="ruvA"
                     /locus_tag="VEL68_21275"
     CDS             2148..2750
                     /gene="ruvA"
                     /locus_tag="VEL68_21275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXZ38575.1"
                     /translation="MIAQIRGRLIHKEPGSVVVEANGLGYRVFVSLTTFYELPDLEVA
                     VTLHTYTHVREDALHLFGFSTPLEKELFQILIGVTGIGPKLALNILSGITPGELLQSL
                     DQRDMNRLQSVPGIGRKMAERLVVDLQEKARKVRTRMALQPAVARPTDGLAEDVVSAL
                     LNLGYKKGQAEKAVEIVFRQSPDLTLEKALKESLKILASI"
     gene            2755..3831
                     /gene="ruvB"
                     /locus_tag="VEL68_21280"
     CDS             2755..3831
                     /gene="ruvB"
                     /locus_tag="VEL68_21280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXZ38576.1"
                     /translation="MATRKKSSKFILDEEEKNSPAVRRVQPEVSEEDLRNEASLRPRT
                     LEEYVGQEEVKGNLRVFVEAAKIRGEALDHVLFSGPPGLGKTTLAYIIASELRVNVKA
                     TSGPVIERPGDLAAILSNLKEHDILFIDEIHRLNHVVEEILYPAMEDFQIDILIGQGP
                     SARSIKLNIPPFTLVGATTRAGLLTFPLRDRFGISFRLEFYSPIELEKILMRSARILK
                     VELNPSGAQEIAKRSRGTPRVANRLLRRVRDFAQVKGGGKIDQAISDQALKLLEVDEM
                     GLDKMDRRILLTILEKFNGGPVGLDTLAVAVNEEKETLEDVYEPYLIQVGFLDRTPRG
                     RRVTPLAAGHMGKQTQHFPQERLL"
     gene            3858..5510
                     /locus_tag="VEL68_21285"
     CDS             3858..5510
                     /locus_tag="VEL68_21285"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXZ38577.1"
                     /translation="MVEIKESKNPAGRTKYIFVTGGVLSSLGKGLTAASTGALLECRG
                     LKVTLQKLDPYINVDPGTMNPFQHGEVFVLDDGAETDLDLGHYERYTSARLTRRHNLT
                     TGQIYYSVITKERRGDYLGATVQVIPHITDEIKRNILAISNDADVAIIEVGGTVGDIE
                     SLPFLEAIRQLKSDLGRQNSINIHVTLVPYIKTSGELKTKPTQHSVMKLREIGIQPDI
                     LICRTEQILPPEIKAKISLFCNVDRDAVVTAKDVESIYEVPLLFHQEGLDEKVVQLLN
                     IWTRAPVLEKWEEIVESIKNPRGSVEIAIVGKYVHLKDSYKSLNEALVHGGIANRLRV
                     NLHFVDSEVVEKEGPEKLLKGADGILVPGGFGNRGIEGKILAAKYAREMGVPYFGICL
                     GMQLALVEFGRHVCGLERANSTEFDFESPHPIIALMEEQKAIQDKGGTMRLGAYPAVL
                     EEGSFAHQAYGEAQISERHRHRYEVNNEYRAQLEAKGILFSGLSPDGKLVEICELPGN
                     PWFLGCQFHPELKSRPHAPHPLFREFISASYKEKRKKRRKSS"
     gene            5507..6358
                     /gene="kdsA"
                     /locus_tag="VEL68_21290"
     CDS             5507..6358
                     /gene="kdsA"
                     /locus_tag="VEL68_21290"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXZ38578.1"
                     /translation="MTSISLPSVKEVRIGPVTLGGRRPLALIAGPCVIESEGACLEVA
                     AWLKELTRSLGIPLIFKSSYDKANRSSLRSYRGPGLDQGLKVLSRVKRELEIPVLSDV
                     HRFEEVKEAAQVLDVLQVPAFLCRQTDFVMAIAQAGKVVNVKKGQFLAPWDLQNIVEK
                     IESAGNQSILLTERGVSFGYNNLVADMRSLVILRQTGYPVIYDVTHSLQLPGGLGTSS
                     GGQREYIPPLARAGVAAGIDGLFMEVHPNPSEALCDGPNSQPLKEVGPLLEQLVALDR
                     IVKTQRA"
BASE COUNT         1531 a         1767 c         1928 g         1261 t
ORIGIN      
        1 gcctcatttt gcggaagcaa ggggggatct ccgaatggat ttcttttccc gccgatgctt
       61 tttccgcgca acgcccttgc ttccgggcca caagcttgct ttttagcaga aaagagactc
      121 gcaaaagtga aggtgggcaa gtgagcaagg tgaattgaca tccttctgcc tttccgataa
      181 actttgatgt aaattctaag agatggcctg ttaagagttg aattttgaaa atatttgaaa
      241 cccccatcga aatgggctat ccgatttccc ggttttgagg cggggtcgat gaggttaggc
      301 tacattcatg ggggtggaga ggagacacta tgttgaattt ttttaagagg ccgaaggtca
      361 tggcggttct gatcgcgatc gccgcctgtt ttctcctgat cctgtatagc tatttcgtgg
      421 cggacacggt ccagacgata attactgacg cccagatgac caaggtggac gggaagtaca
      481 tgatcgccac cgaataccgg cctttcgtca actatgacgc ctggtaccgg ttcaagttcg
      541 attccggaac catccagaac caggcagtgc ggctaagggg gaaaacggtc aagataaaga
      601 aatacgggtg gaggatcccc ttcttttccg agtatgagaa tgtggtgaaa atcgaagagg
      661 tgaagtgagt caaggccttt tggtttcagc ctttcaagcc tattgaatcc ggtctttttt
      721 gcttttgcaa agcctctcgc cttatgctat aaaagatgaa ttaaaaaaga aggggagata
      781 gcctcatgtc cggccactca aaatggagca cgatcaagca caagaagggc gcggccgatg
      841 ctaagcgggg aaaaaccttc accaagctga tcaaggaaat cacggtggcc gcgcgcacgg
      901 gcggagggga cccaggcggg aaccctcgcc tgcgaagtgc gatcaccgcc gcgaaggctg
      961 agaacatgcc caaggacaat atcgaccggg ccatcaagaa aggcacgggc gagcttcccg
     1021 gagtatccta cgaagaggcg accttcgaag ggtacgggcc cgggggagtg gcgattctgg
     1081 tggaaatcct ttcggataac cgaaaacgga cggtggcgga cattcggcat atcttctcga
     1141 agaatgccgg caacctgggg gaggccggct gcgtttcctg gatgttcgga aagaaggggc
     1201 tgatcgtctt cgaaaaagag aaggtggatg aagagaagct gatgaacgtc gccctcgacg
     1261 ccggggcgca ggacgtgcgg gaaacggaca aagaattcga agtggtctgt gatccttccg
     1321 attttgaaaa ggtaaagaaa gccctggaac aaacccagct caaacccctg ctcgcggaaa
     1381 tcaccatggt tccccagtcg acgatcaagc tggcggggaa agaagcccag cagatgcttc
     1441 gtctgatgga aagcctggag gattcggacg acgtccagca cgtctacgcc aatttcgaca
     1501 ttcccgatgc cgagatggag cagctcagtg cgtaagggag gtgcccccgg ttcatggccc
     1561 ctccttccgg ggagccagag cgaaggcatc cgtatcttag gcgtagaccc ggggatggtg
     1621 agcacgggtt tcggcctgat cgaattcgaa aaacaaggtt ccctcctgta ccgcgagagc
     1681 ggggttcttc gcccccgggg aacttctgtc cctcctcgtc tgcagcagat cttccgcggc
     1741 ctcaccctcc tgattgaaac cgcgaagcct cgcttcatgg cggtggaacg cccctttttc
     1801 ggcaagaacg tgaagagcgc catgctcctc gggcaggccc gggggaccgc gctcctggca
     1861 gcggcggaag ccggcctgga ggtccatgaa atcagccccc tggaggtcaa acaggcggtg
     1921 gtaggatacg gccgggcgga caaaggacag atccaggcca tggtccggtc catcctggga
     1981 atccgccaga ctctgaccgc ggacgcagcc gacgcgctgg ccgtagccct ctgcctggcc
     2041 cattctctgc ctttaaagac ctccctgcag gaggccctgt tgaaggattc tcggggggaa
     2101 cggcgccggt ctacgaggtg gacggaaaag gatttgggag actttcggtg attgctcaga
     2161 ttcggggacg gctcattcac aaagaacccg gctcggtggt ggtggaggcc aatggtttag
     2221 gttaccgggt ctttgtctcc ctgaccacct tctatgaact ccccgacctg gaggtggccg
     2281 tgaccctaca tacctacacg catgtccggg aagacgccct ccatctcttc gggttttcca
     2341 cccccttgga aaaagagctc ttccagattt tgatcggagt cacgggcatc ggtcccaagc
     2401 tggccctgaa cattctttcg ggtattactc ccggggaact cctccaatcc ctggaccagc
     2461 gggacatgaa ccgccttcag tccgttcccg gcatcggacg gaagatggcc gagagattgg
     2521 tggtggacct ccaggagaaa gcccgcaagg tccgcacccg catggccctg cagcccgccg
     2581 tagcacggcc aacggatgga ctggcggaag atgtcgtctc cgctctgttg aatttgggat
     2641 ataaaaaggg gcaggcggaa aaagcggtgg aaatcgtgtt ccgccagtcc ccggatttaa
     2701 ccctggaaaa ggctttaaag gaaagtctga aaattctcgc ctccatttga aaatatggcc
     2761 acccggaaaa agtcctccaa attcatcctc gacgaagaag aaaaaaattc ccccgccgtg
     2821 cgacgggtcc agcccgaagt gagcgaggaa gacctgcgca acgaggccag cctgcggcct
     2881 cgtacgctgg aagagtatgt ggggcaggaa gaagtgaagg gcaatctgcg ggtctttgtg
     2941 gaagcagcca agatccgcgg ggaggcgctg gaccacgtcc tcttcagcgg tccccccggc
     3001 cttgggaaga ccaccctggc ctacatcatc gcctccgaac tgagggtcaa cgtcaaggcc
     3061 acttccggac cggtcatcga acggccggga gatttggcag ccatcctcag caacctgaag
     3121 gaacacgaca tcctcttcat cgatgagatc catcggctga accacgtggt ggaggagatt
     3181 ctctacccgg ctatggagga ttttcagatc gacatcctca tcggccaggg gcccagcgct
     3241 cgctccatca agttgaacat tcctccgttc accctggtgg gcgcgaccac ccgggccggt
     3301 ctccttacct tccccctgcg cgaccggttc ggaatcagct tccggctgga gttctactcc
     3361 ccgatcgagc tggagaagat cctcatgcgc tctgcccgga ttctgaaggt ggaactgaac
     3421 ccttccggcg cccaggagat cgccaagagg tcgcgtggaa cccctagggt ggccaaccga
     3481 ttgctgcgac gggtgcggga ttttgctcag gtgaagggag gagggaaaat cgaccaggct
     3541 atttccgatc aggccctgaa actcttggaa gtagatgaaa tggggctaga caagatggac
     3601 aggaggatcc ttctcactat cctggaaaaa ttcaacgggg ggccggtggg actggatacc
     3661 ctggccgtgg cggtgaatga ggaaaaggag accctcgagg acgtgtacga accgtacctg
     3721 atccaggtgg gatttctgga ccggactccc cgggggagga gggtgactcc cctggcggcc
     3781 ggtcatatgg gaaagcaaac ccaacacttt ccgcaggaac gcctgctcta aatcgactca
     3841 ctggggatgg gaaaacaatg gtagagatca aagaatccaa gaatccagcc ggacgaacaa
     3901 aatacatctt tgtcaccgga ggggtccttt cctccctggg caagggtctg accgccgcat
     3961 ccaccggggc tctcctggaa tgtcgggggc ttaaagtgac cctgcagaag ctcgaccctt
     4021 atatcaacgt ggaccccggg accatgaacc ccttccagca cggggaggtc tttgtcctgg
     4081 acgacggggc ggagactgac ttggacctag ggcattacga gcgttatacg tcggcccgct
     4141 tgacccggcg gcacaatctg accaccggcc aaatctatta ctcggtgatc accaaagaga
     4201 gaaggggaga ctacctgggg gccacggtcc aggtcattcc ccacatcacc gatgagatca
     4261 agcgcaacat cctggccatt tccaatgatg ccgacgtggc gatcatcgag gtggggggga
     4321 ccgtgggaga tatcgaatcg ctcccgtttt tggaagcgat tcgacagctg aaatccgatc
     4381 tgggacgtca gaactcaatc aacatccacg tgactctggt cccttacatc aagacctcgg
     4441 gggagttaaa aaccaagcct acgcagcata gcgtaatgaa gctccgggag atcggaatcc
     4501 aaccggacat cctgatctgc aggaccgaac agatccttcc tccggaaatc aaagccaaga
     4561 tctccctttt ctgcaatgtg gaccgggacg cggtggtcac cgccaaagac gtggaatcca
     4621 tctatgaggt tcccctcctt ttccatcagg agggactcga cgaaaaggtc gttcagctgt
     4681 taaacatctg gacccgggcc cccgtcctgg aaaagtggga agagatcgtg gagtccatca
     4741 aaaaccctag gggcagcgtg gagattgcca tcgtgggcaa atacgtgcac ctcaaggatt
     4801 cctataaatc cctgaacgaa gccctggtcc acggcggaat tgccaaccgg cttcgggtca
     4861 atcttcactt tgtggactcc gaagtggtgg aaaaggaagg gccggagaag ttgctaaagg
     4921 gagcggacgg aatcctcgtg cccggggggt tcgggaaccg gggaatcgag gggaagatcc
     4981 tggccgcgaa atacgccagg gaaatggggg tcccctattt tggaatctgc ctgggaatgc
     5041 aactggcctt agtggaattc ggccgccatg tctgcgggct ggaaagagcc aacagcaccg
     5101 agttcgactt cgagtccccg catcctatca tcgccctcat ggaggaacaa aaagcgattc
     5161 aggataaggg gggcacaatg cgactgggtg cctacccggc ggtcctggag gaagggtctt
     5221 tcgcccatca agcctacgga gaagcgcaga tttccgaacg ccaccggcat cgctatgaag
     5281 tcaacaatga gtaccgggcg caactggagg ccaaagggat cctattcagc ggcctctccc
     5341 cagacggcaa gttggtggaa atttgcgaat tgcccggtaa cccttggttc ttgggctgcc
     5401 aatttcatcc ggagttgaaa tcccggcccc atgcccccca tcctttattc cgcgaattca
     5461 tctcagcctc ctacaaggag aagcgaaaga agcggagaaa gtcatcatga cctcaatctc
     5521 ccttccatcc gtgaaagagg tccgaatcgg gcccgtaacc ctgggaggac gaaggcctct
     5581 ggcgctgatt gccgggccct gcgtcatcga gtccgagggc gcctgcctag aggttgccgc
     5641 ctggttgaag gaattgaccc gatccctggg gatccccctg atttttaaat cctcctatga
     5701 caaagccaac cgaagctccc tgcgctccta tcggggtccc ggcctggacc aggggctgaa
     5761 ggtcctgagc agggtgaagc gggagctgga aatcccggtc ctttcggacg tgcaccgctt
     5821 tgaagaagtg aaagaagccg cccaagtcct tgatgtcctc caggtgccag cctttctatg
     5881 ccggcagacc gatttcgtga tggccattgc ccaggccgga aaagtggtca acgtaaaaaa
     5941 ggggcagttt ctggccccct gggacctgca gaatatcgtg gaaaagatcg agtccgctgg
     6001 gaaccagtcg atcctgctca ccgagagggg ggtctccttc ggctacaaca acctggtggc
     6061 ggacatgagg tccttggtta ttctacggca gacgggctac ccggtgatat acgatgtaac
     6121 ccacagcctt cagcttcccg ggggcttggg cacctcatcg ggagggcaac gggaatacat
     6181 tcctcctttg gccagagccg gggtggccgc ggggatcgat gggcttttca tggaagttca
     6241 tcccaaccct tcggaagccc tctgcgacgg gcccaactca cagcctttga aggaagtagg
     6301 ccccctcctg gagcagttgg tggctctgga tagaatcgta aagacgcaaa gagcataggg
     6361 aatagggcag aagtgcaaag caaagaaaaa gggaccgaag gcgcatagcg atagcgccga
     6421 acccaaagcg cagggagaaa attattcctg gggagagaac gggaggaatg gcacggaaag
     6481 gctggaa
//
LOCUS       DASPRR010000580         4910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_623150, whole genome shotgun sequence.
ACCESSION   DASPRR010000580 DASPRR010000000
VERSION     DASPRR010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623150"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VEL68_21295"
     CDS             <1..180
                     /locus_tag="VEL68_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38579.1"
                     /translation="ASFCGSKGGSPNGFLFPPMLFPRNALASGPQACFLAEKRLAKVK
                     VGKDKNHLTEKGKLI"
     gene            211..1152
                     /locus_tag="VEL68_21300"
     CDS             211..1152
                     /locus_tag="VEL68_21300"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXZ38580.1"
                     /translation="MGTDVLLRTEFKEIPVLHRGKVRDIYDLGKHLLIVATDRISAFD
                     VVLPNGIPHKGRVLNQISAYWFKTLEDIVAHHMVTPELERFPEIPKAVAEQLRGRAMV
                     VKKAKSLPVECIVRGYLSGSGWNEYQEKGSLSGIPLPPGLKESQKLPASLFTPSTKAE
                     VGIHDENIDFATATQKLGKKLAEEVRSASLQIYQKAAAMAEKRGIIIADTKFEFGLDE
                     AGKLILIDEALTPDSSRFWPTDGYSPGGAQKSFDKQFVRDYLLSIRWDKKPPAPNLPD
                     EVIQKTSEKYIEAYERLTGKQLEPQPTKSKLQTSKSK"
     gene            1342..2394
                     /locus_tag="VEL68_21305"
     CDS             1342..2394
                     /locus_tag="VEL68_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXZ38581.1"
                     /translation="MGKTGIVKDDRYMDHVADGYHPESHQRLEVLYQMLKEPAMKGNY
                     AEVPPRIATQEELELIHMPNYIRLVASTAGRSMTMLDPDTYACAKSYDAARLAAGGTM
                     AAVDKVLSGEVNNAFALIRPPGHHAETNRAMGFCLFNNVAIAASYAIVTHNLKRVLII
                     DWDLHHGNGTQHSFYERADVLYFSTHQYPYYPGTGFFTETGSGAGKGFTVNVPLNPGP
                     GDAEYFLIFEEILEPIAVEFKPDIIFVSAGFDIYYRDPLGGMQVTPQGFANLAKVILE
                     FAQETCKGRVVFALEGGYHLEGLRDSAREVLKTMRGEILAQGRDEEVRKQVDHRLIDP
                     VIKKVKEAQKPYWKIG"
     gene            2410..3150
                     /locus_tag="VEL68_21310"
     CDS             2410..3150
                     /locus_tag="VEL68_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ38582.1"
                     /translation="MSELEVHFLGSGDAVGSGGRFQACVLVRSGGESFLIDCGASSLI
                     AMKRFSVDPSSIGRVLLSHLHGDHFGGIPFLILDGQFSRRTRPLTIAGPKGVESRVMA
                     AMEVLFPGSTKVERKFPISFAEFHPEQEMTLGPLSVIPYEGVHPSGSPSLSLRIQTAG
                     RVIAYSGDTEWTEALPAVGRDADLFICEAYYFEKKIKYHMDYRTILDRLPRIGCKKII
                     LTHLNQDLLSRLAEVKIDWAEDGKKIII"
     gene            3590..4231
                     /locus_tag="VEL68_21315"
     CDS             3590..4231
                     /locus_tag="VEL68_21315"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38583.1"
                     /translation="MMEKTLGQILLEKNMITQAQLDLALRKQKQQKGKYLGQILIEMG
                     LVSQEKINKVLDTFNKRKRIGETLIDLEIITLEQLETALRKQKELQTQGVRKPLGTVV
                     LELGFTNYDSYLLALSKHFNMPIVYLETFYPTPTLQRALGEKYAMKNRIVVLENTPSR
                     IKVALAEPTHRILEEVQKAVPFGKTVEFYLANPYEVDSCLRKKFDPFAVTRYR"
BASE COUNT         1244 a         1279 c         1310 g         1077 t
ORIGIN      
        1 gcctcatttt gcggaagcaa ggggggatct ccgaatggat ttcttttccc gccgatgctt
       61 tttccgcgca acgcccttgc ttccgggcca caagcttgct ttttagcaga aaagagactc
      121 gcaaaagtga aggtgggcaa ggataagaac cacttgacag aaaagggaaa actgatttaa
      181 tgggtattaa aaatggaggg aaaggtcttt atgggaaccg atgttctgct gcgcacggag
      241 ttcaaggaaa tccccgtcct gcatcgggga aaggtccggg atatttatga tttgggtaaa
      301 catctcttga tcgtggccac tgaccggatc tccgccttcg acgtggtcct ccccaatggc
      361 atcccccata aagggcgggt tctcaaccag atatcagcct attggttcaa aactttggag
      421 gatatcgttg ctcatcatat ggtgaccccc gagctggaaa gatttccaga aatccccaag
      481 gccgtggccg aacaattgcg cggccgggcc atggtcgtga agaaggccaa gtccttgcct
      541 gtggaatgca tcgtccgagg atacctatcc gggtccggtt ggaatgagta ccaggagaag
      601 ggatcgctca gcgggattcc tctcccgcct ggtttgaagg aatcccaaaa attgccggcc
      661 tccttgttta ctccttccac caaggctgaa gtgggaatcc acgatgagaa tatcgatttc
      721 gccacggcga cccagaaact tgggaagaaa ttggccgaag aggttcgctc ggcgagcctg
      781 caaatatacc aaaaggctgc ggccatggca gagaagaggg ggatcatcat cgccgacacc
      841 aaattcgaat tcggattgga tgaggcaggg aaattgatcc ttatcgacga ggcgttgacc
      901 cccgattcct ccaggttctg gcccacggac ggatattccc caggaggggc gcagaagagc
      961 ttcgacaaac agttcgtccg ggattatttg ctctctatcc gctgggacaa gaagcctccg
     1021 gcccccaacc tgcccgatga agtcatccaa aagacctcgg agaaatatat cgaggcgtat
     1081 gaacggctga cggggaagca gttggaaccc caaccgacaa agagcaaact ccaaacttca
     1141 aaatcaaaat aaatgcgaac tccccaaagc ccaatggtcc aaactgagga aaaaccaaac
     1201 gcccttccgg gatcggaata cggtttttat attttggata ttggtactca gcattcgttt
     1261 aggatttggc gcttgatttg aaggacccct gaagggggga aatatccctt tcaataactc
     1321 ggaagatcgg agcggaaaga aatgggtaag accgggatcg taaaagatga ccgttatatg
     1381 gaccatgtgg ccgacggcta ccatccggag tctcaccaac ggctggaagt gctctaccag
     1441 atgttgaaag agccggccat gaaggggaat tacgcggagg tcccgccccg gatagccacc
     1501 caggaggaac tggagttgat ccacatgccg aactacattc gtctggtggc ctccaccgcc
     1561 ggccgatcca tgaccatgct ggacccggac acctatgcct gcgccaaatc ctatgatgct
     1621 gccaggctgg ccgccggcgg aacaatggct gcggtggaca aggttttaag cggggaggtc
     1681 aataatgcct tcgccttgat ccgcccgccc gggcatcatg ctgaaaccaa ccgggccatg
     1741 gggttttgcc tcttcaacaa cgtggccatt gctgccagtt atgccatcgt tacccacaac
     1801 ctgaagagag tgctaattat cgattgggac ctacatcacg gtaatgggac ccaacattcc
     1861 ttttatgaaa gagcggacgt tctgtatttt tccacccatc aatacccata ctatcccggg
     1921 accggctttt tcaccgaaac cggaagtgga gccgggaaag ggttcacagt caatgtgccc
     1981 ctgaacccgg gtcccggaga cgccgagtat ttcttgatct tcgaagaaat cctggaacct
     2041 attgccgtgg agttcaaacc ggacatcatc ttcgtttccg ccggatttga tatttattac
     2101 cgcgatcccc tgggcgggat gcaggtcact ccccaaggat tcgccaacct ggccaaggtc
     2161 atcctggaat ttgcccagga aacctgcaaa ggaagagtcg ttttcgccct cgagggagga
     2221 tatcacctgg aggggctgcg cgattcggct cgggaagtgc taaagaccat gcgcggagaa
     2281 atcctggccc aaggcaggga tgaagaagtt cgaaaacagg tcgaccatcg gttgatcgat
     2341 ccggtcatca agaaagtgaa ggaggcccag aagccctact ggaaaatcgg ttgaaaggaa
     2401 aagaaagtga tgtccgaatt ggaggttcat ttcctgggtt cgggagatgc ggtgggaagc
     2461 ggggggcgtt tccaggcctg cgtcctcgtc cgctccggag gagagagttt tctcatcgac
     2521 tgcggcgcct cttcgctgat cgccatgaag cggttcagcg tggacccttc ttccatcggc
     2581 cgggtcctcc tttctcacct ccacggagac cacttcgggg gaattccctt cctcatcctg
     2641 gatgggcaat tcagccggcg aacccgcccc ctcaccatcg ccggccccaa aggggtagaa
     2701 agccgggtaa tggcagcgat ggaagttttg ttccccggct caacaaaggt ggagaggaaa
     2761 tttccaatat cctttgcgga attccatcct gaacaagaga tgacccttgg gcccctctcc
     2821 gtgattccct atgaaggggt gcatcccagc gggtctccct ctctttccct tcgcatccaa
     2881 actgccggaa gagtgatcgc ctattccgga gacacggaat ggacggaggc tctaccggct
     2941 gttggccgtg atgccgacct ctttatttgt gaagcttact atttcgagaa aaagatcaag
     3001 tatcatatgg attaccggac gatcctggac cgtcttccgc gcatcgggtg caagaagatc
     3061 atccttaccc atttgaacca ggacctgctg tcccggctgg cggaggtgaa gatcgattgg
     3121 gcggaggacg gcaagaagat cattatataa atatgccttg gtactcgcct accccaccct
     3181 tcaatccccg atcgggggag aactgcagag aaaggccatt tctaaatttc ctttcatgga
     3241 tactcgggcc atggccccgg tgaaggtcac cttccgtcca aaaggagcgt tcaagtttgt
     3301 aaaaatattt gacgaaaccg tcaaaaatat tgctaatcct taaaaaaatt aaagggtcct
     3361 cctcggctat taaaaaaaac attaatgatt ttaaatagat atcttacaaa aggagtgcaa
     3421 aaagcgggca tcaggtttgc atgaaaccat taggatcaaa gggaaaatat ggattttttt
     3481 cgtatttttg agcgctaaag gatagtttta agaccaaaga acctgccatt cggggttctg
     3541 attgaaggga aatcccccta ggggaagggg aagccaaaaa gaaagagcta tgatggaaaa
     3601 aactttggga cagattttgc tcgaaaagaa tatgatcacc caagcccaat tggacctagc
     3661 cctgagaaag cagaaacaac agaaggggaa gtatttgggg caaattctga ttgagatggg
     3721 attggtttcc caggaaaaga tcaacaaagt tctggacaca ttcaacaaga ggaaacgaat
     3781 cggagagacc ctcatcgacc tggagatcat cactctcgag caattggaaa cggccctgag
     3841 gaagcaaaag gaactccaaa ctcagggagt tcgaaaaccc ctggggacag tggtcctgga
     3901 attggggttt acgaattacg acagttacct gctggctctc tccaagcatt tcaacatgcc
     3961 catcgtttac cttgagacct tttatcctac ccccactctt caaagggcct tgggagaaaa
     4021 gtatgctatg aagaaccgga tcgtggttct ggaaaacacg ccttcccgaa tcaaggtggc
     4081 cctggctgaa ccgacccatc gcatcctgga agaagtccaa aaagctgttc ccttcgggaa
     4141 aaccgtggag ttctacctgg ccaacccgta cgaggtagat tcctgcctcc gcaaaaaatt
     4201 cgatcccttt gcagtaaccc gctatcggta aagactcgaa gactcctcaa gcggattttt
     4261 ctcgacccgc ggccacccac ccctcctttc ccgcgtgcta taatttcatt ctgggaaacc
     4321 cccggctctg ctgggggact caaagagttt gacatttccg ggatgcctga gttctggccc
     4381 gggggcaggg atctggggtt caaaaagcat cggcggggcg atccctattc ctggaggctg
     4441 ggactggctt ccgcttgagc cggcggcccg atcgggcgac cggccggtcg cccctacaag
     4501 gacaagacct tccacggggt tgcgggaatg gctggccgac ccggcccatg aaatgaagga
     4561 tgcctcattg tttttggacc ccagaccctc gctcccgggc ctggtcacca gtccaaggcc
     4621 acctttaggt ggggaccatt tcaatccgcc cagggtcggg tcttatcgac tttgagcggt
     4681 tcaggggtat tgcatgcctc cacctctggt ggaggtcatg acttaaaaaa ttggatttgg
     4741 catttcatct tccgcaggaa gaacagccta ggttaaaaat gagtgtgctc ttgatccatc
     4801 ctcccgtcag caaaccctgt gaaccacccc cgggaattgc caggctttgc ggggcccttc
     4861 aggccaatga agtggcagcc gctgtcctgg atatgaacct tggggggctc
//
LOCUS       DASPRR010000581         7334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_623225, whole genome shotgun sequence.
ACCESSION   DASPRR010000581 DASPRR010000000
VERSION     DASPRR010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623225"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..1243
                     /locus_tag="VEL68_21320"
     CDS             476..1243
                     /locus_tag="VEL68_21320"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HXZ38584.1"
                     /translation="MKTQPAKGKKGRRVLVISAHPDDVDFGCGGTMARWAQEGAEVFY
                     CICTSGEKGTDDPMLPCHALAPLREKEQRAAARVVGAKEVVFLRKPDGELQYSLEFRG
                     DLVRVIRQVRPHTVFTHDPANRSFDVQYLFHADHRVVGELAFDAVYPAALNHNFYPSH
                     MVEGLSPHAVSEVYFFASAQPDTWIDIGPTLHLKLKALRCHASQIKNPRMMEQMVRTW
                     FGAWGREKGFAYAERFRRLEVFQNPGERLKKVLKTLK"
     gene            1415..2086
                     /locus_tag="VEL68_21325"
     CDS             1415..2086
                     /locus_tag="VEL68_21325"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXZ38585.1"
                     /translation="MDSWNWKDRILLQIIPRIYVWVLRLFSLTVRKEYLHEEYPKEFW
                     NQGLHIIISFWHQRLMMMPFLRHSGRVGMLISQHRDGEFIARAVNQFGVNPVRGSTTR
                     GGLSALRGMIRFFRTGANLAITPDGPQGPKHVVQIGVIELARQTGAPILPVTYSASRK
                     KVLGSWDNFVLPIPFCKVAYLWGEPLLIPRDADKDRMEESRQLLQERMRQITEEADRI
                     FQRFS"
     gene            2373..3629
                     /gene="fabF"
                     /locus_tag="VEL68_21330"
     CDS             2373..3629
                     /gene="fabF"
                     /locus_tag="VEL68_21330"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXZ38586.1"
                     /translation="MKTRVVITGLGVVSPIGIGVKEFWKAAMEGRSGVTAIKSFDPFP
                     LEGFRSRVAGQIENFDLNQYLDFPQGARVDRFGQFALVAAKEALADSGLRMDREDAHR
                     VGVIVGAGMGGMVMGEREVTQLYQQLRPHRVHPNLIPAITLNSASGILAMAYGAKGPN
                     LTISTACSSSAHALGQALNCIRSGQADTVMAAGADASITPLVFAGFCSLRALSTKFNE
                     TPAKASRPFDRLRDGFVLGEGAGALILESLSHAKKRKARVYAEVAGYAATSEAYHMVI
                     PREDGVEIAETIRLALKDAGIAPAQVDYINAHATSTPVGDNVEIKGIRQVFKGRAEKV
                     AINATKSLIGHTLGAAGAIGGVATALSIETGQIHPTINYDEPDAECKLPGISRSAQER
                     PVKVALVNSFGFGSNNAAVVFRKPSL"
     gene            3698..4000
                     /locus_tag="VEL68_21335"
     CDS             3698..4000
                     /locus_tag="VEL68_21335"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXZ38587.1"
                     /translation="MAADSRIADQIITALAEYLKRDKKSILLENRLRDDLGLDSIATI
                     ELLFKLEEVFDLQIPDEDLKGLVTVGHVVKYLQKRLAPPPSKKTPQPAAKTKRPKK"
     gene            4008..5093
                     /gene="lpxD"
                     /locus_tag="VEL68_21340"
     CDS             4008..5093
                     /gene="lpxD"
                     /locus_tag="VEL68_21340"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXZ38588.1"
                     /translation="MSPYRLPTPLSLSQVQEAIGGEIVGPPETLVSGISSLEEAEPGD
                     LSYCSSDRYLKAALQSRAGAFLVNRPIPELKRPQLVVPNPAYEFARIAQRFFTGPYRP
                     RGIAKEAICGVDVQIGPDPSIWPNVTLGDRVKIGARVTLYPGVFIGSDSRVGDDSLLY
                     PNVVVREGCTIGSRVIIHSGTVIGSDGFGYVQHEGRNYKIPQLGGVIVEDDVELGANV
                     AVDRATFGQTVVKRGTKVDNLVQIAHNVTVGEHTILVAQVGIAGSTSIGHHVMIGGQA
                     GLADHVRVGDSAMIAARSGVNRNVASQEIVGGAPVMPFEVFLKSHALLPRLPEFRQQL
                     RELKERVQVLEKKDVGQEKPKKPRRKP"
     gene            complement(5291..7078)
                     /locus_tag="VEL68_21345"
     CDS             complement(5291..7078)
                     /locus_tag="VEL68_21345"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXZ38589.1"
                     /translation="MYKHRKRISDILMESGLITPHQLEHALMLQKSKNKRLGKILVEL
                     GYVSETQIAEALAKQLSLPFVDCSQYRISEEIKSLVPKEVVEKKLVIPLELQGRSLLL
                     AMADPLDYETIGELAFINNLNIKHVVACETNLLEAIEKNYGAEENVWDLVKDMPKFEE
                     VEFKKESPEEEISVQSLYKSSEAPAIVKLVTMIIVDAVQSRASDIHIEPREKYVLVRY
                     RVDGELRDILRVPKNVQNPVISRIKIISNMDITNRRLPQDGNSYLKVGEKEVDLRVST
                     LPSIYGEKVVIRLLNRNTGLIPVAHLGIPEFIFNQLIEIFSQPQGMLLVTGPTGAGKT
                     TTLYAWLNQLRSETENIITVENPVEYKFEGITQVSINEAVGYTFSTALRSILRQDPDI
                     IMIGEVRDLETAEIAIRAALTGHLVLSTLHTNDTVSTVMRLVDIGVANFLVSSSVSGV
                     LAQRLVRRICPKCKVEVDPPNASLYKDYPPIEHFYRGKGCPHCYYTGYYGQIGVFELL
                     RINTKIRRLIAKNVYEDELWDAARESGMKTLFEEAWGKVREGITTIEEVMAKIPEQYI
                     ENVERSHISKENDKELLLPPAEKDSYSGP"
BASE COUNT         1569 a         2063 c         2081 g         1621 t
ORIGIN      
        1 ggcctccagg aggatcaggt ccagaagctt ttgcaggtcg tggacgctgg agagggcgat
       61 tcccacactc aagagctgct caaattcttc ggccaaacgg gactggtcca tggattttcc
      121 tctgcttgaa tgaagcctgg aaggagaaaa gatggctgat tatagactgg tcccctgtca
      181 aaggcaagaa ggatcgaggg gtttcccgga ttataatttg acaccccacc cggggacggc
      241 ctatatttat taaaggcatc aaattgcaat cccactgaag cgggaccaaa tcccacaatg
      301 aaaaacaagc aattttctct cgccgcgaac gaagaggcgg tatagaaaaa aatccataat
      361 ttttgaaaat cccgaattca attctctctc tccatgccct gcggcctcga atgaagtagg
      421 cgagagataa ctttatctgg acatctggaa ttggggatcg accaaggagg atggattgaa
      481 aacgcaacct gccaaaggga aaaaagggcg gcgagttctg gtcatcagcg cccatccgga
      541 tgatgtggac ttcgggtgtg ggggaaccat ggcccggtgg gcccaggagg gagcagaggt
      601 cttctactgc atctgtacca gcggcgaaaa ggggaccgac gaccccatgc ttccctgtca
      661 tgccctagcc cctctacggg agaaagaaca gcgggccgcc gctcgggtag ttggagccaa
      721 ggaagtcgtc tttctccgga aacccgacgg cgagcttcag tattctctgg aatttcgggg
      781 agaccttgtt cgggtgatcc gacaggtccg cccccacacg gtcttcaccc acgacccggc
      841 caaccgctct tttgatgttc agtacctttt ccatgccgac catcgggtgg tcggggagct
      901 ggccttcgac gccgtttatc ccgcggctct gaatcacaat ttttatccct cccacatggt
      961 ggaagggctg tccccccatg cggtttcgga ggtctacttc ttcgcttcgg cccagccgga
     1021 tacctggatc gatatcggac cgaccctgca cctgaagctc aaagccctcc gatgccacgc
     1081 cagccagatc aaaaacccca ggatgatgga acagatggtg cgcacctggt tcggcgcctg
     1141 gggtcgggag aaaggcttcg cctatgccga gcgcttccgg cgtttggagg tcttccagaa
     1201 tccgggggaa cgtttgaaga aggtgctaaa aaccctgaag tgaaagtaaa cagcgaagcg
     1261 caaaatccga aattgaagat aaattcaaat gggcgcagta ccaatccgca aacgatggag
     1321 tcgtagtctg gagaaaatag gtcatttgaa ttttgggttc gtttcctccc gcccttcgcg
     1381 ggattcgata ttcaaatttt gaatttgaac cgttatggac tcatggaatt ggaaagatag
     1441 gattctcctt cagatcatcc cccgaatata cgtctgggtt ctccgccttt tctccctgac
     1501 cgtccggaag gaatatcttc atgaggagta ccccaaagaa ttctggaacc agggcctcca
     1561 tatcatcatt tctttctggc atcaaaggct gatgatgatg cctttccttc ggcactcggg
     1621 aagagtgggc atgctgatca gccagcaccg ggacggcgaa ttcatcgccc gcgcggtaaa
     1681 tcaattcggg gtgaacccgg tccggggctc aaccacccgg ggcggcctct ctgcgctgcg
     1741 ggggatgatc cgctttttcc gtaccggcgc caatttggcc atcacgccgg acggccctca
     1801 agggcccaag catgtggtcc agatcggggt catcgagctg gcgcgccaga ccggcgcccc
     1861 cattctcccc gtcacctaca gcgcttcccg gaagaaagtg ctcggaagct gggacaactt
     1921 cgtcctccct attccttttt gcaaagtggc gtatctttgg ggggagcccc tgctcattcc
     1981 ccgggatgcg gacaaggatc ggatggaaga aagtcgccaa ctcctccagg aacgaatgcg
     2041 gcaaattacc gaggaagccg accgaatctt tcaacgtttt tcctgaattc ggattccttt
     2101 cgtcccacca tcggggattt tcttgcctat ctttacttta ctttttggca aggaggcgta
     2161 ggtccatata acatcaggga ttgaagagct cggaagaaag gccgcaagct tgttttttcg
     2221 taaaaaggaa caaaagccaa gggggcaagc gggaattttc gcttgacacc gaatttttct
     2281 ccttcctata atccaacata agtggaattt ttctctgatc ggagggaaag ggcctctttg
     2341 agcgacttca ccttgaaaag gatggaccga gaatgaagac ccgtgtggtg attaccggct
     2401 tgggagttgt ttccccgatc ggcatcggag tcaaggaatt ttggaaagcg gccatggaag
     2461 ggcgatcagg ggtgacggcc atcaaatcct tcgacccctt tcccctggaa gggttccggt
     2521 cgcgcgtcgc cggccaaatc gaaaatttcg acctcaacca gtatctcgat tttccccagg
     2581 gagcccgggt ggatcggttt ggccagttcg cattggtggc cgccaaagaa gccctggcgg
     2641 actccggtct ccgaatggat cgggaggatg cccatcgagt cggggtcatc gtgggagccg
     2701 gcatgggcgg aatggtcatg ggagaacgag aggttaccca actgtatcag cagctcaggc
     2761 cgcaccgagt ccaccccaac cttatacccg cgattacttt gaattctgcc tccgggatcc
     2821 tggccatggc ctacggggca aaaggcccca acctgaccat ctccaccgct tgctcgtcca
     2881 gcgcccatgc cctgggccag gccctgaact gcattcggag cggtcaggcg gacacggtta
     2941 tggctgccgg ggccgatgcc agcatcaccc cccttgtttt tgctgggttc tgttccctgc
     3001 gagccctttc cacgaagttt aacgagaccc cagccaaagc ctcccgcccc ttcgatcgat
     3061 tgcgcgacgg atttgtcttg ggagaaggag ccggggcctt aatcctggaa tccctctccc
     3121 acgccaaaaa gcgcaaggcc cgggtctacg ccgaggtggc tgggtacgcg gcgaccagtg
     3181 aggcttacca catggtgatt ccccgggaag acggagtaga gattgccgaa acgatccgtc
     3241 tggccttgaa ggatgccggg atcgctccgg ctcaggttga ttacatcaac gcccatgcca
     3301 cttctacgcc cgtgggagat aatgtggaga tcaagggaat ccgccaggtc ttcaagggcc
     3361 gggctgagaa ggtggccatc aacgccacca aatctctcat tggccatacc ctcggggccg
     3421 cgggggccat tgggggagtc gccacggcct tatccatcga gaccggtcag attcatccga
     3481 cgatcaatta cgatgaaccg gacgccgaat gcaagcttcc cgggatctcc cggtcggcgc
     3541 aggagcggcc cgtgaaagta gccctggtca attcttttgg gtttggaagc aacaatgccg
     3601 cagtggtttt ccgaaaacca tccttgtgaa ccctttgact ccaagggctg tgggcttcgt
     3661 ttggcctgcc gccggggtct ccggaaagga aaggcagatg gccgctgatt cccgtatcgc
     3721 cgatcaaatc attaccgccc tcgccgaata tttaaagcgc gacaagaaat cgatcctcct
     3781 ggagaatcgg ctgcgagacg acctggggct ggactccatc gccaccattg aactcctttt
     3841 taagctggag gaggtctttg atctccagat ccccgatgaa gatttgaagg gtctggtcac
     3901 ggtggggcat gtggtcaaat accttcaaaa aaggctggcc ccccctcctt ccaaaaaaac
     3961 cccccagccg gcggccaaga ccaagcgccc caagaagtga ggatgccatg tcaccctatc
     4021 gactcccaac tcctctctcc ctctcgcagg tacaagaggc tattggaggg gaaattgtag
     4081 gacctccgga gaccctcgtt tcgggaattt ccagcctgga ggaggctgag ccgggagacc
     4141 tttcctattg ttcctccgac cgatacctaa aagcggcctt gcaatcccgg gccggggctt
     4201 ttctcgtcaa ccgtccgatt cctgaactga agcgtcccca gctggtcgtg cccaacccgg
     4261 cctatgagtt tgcccgcata gctcagcgtt tcttcaccgg gccttatcgg ccgcggggca
     4321 tcgccaagga ggccatttgc ggtgtggacg tccagatcgg tccggacccg tccatctggc
     4381 ccaacgtcac cctgggggac cgggtcaaga tcggcgcgcg ggtgaccctg tatcccgggg
     4441 tctttatcgg cagcgattca agggtggggg atgattcctt gctctatccc aacgtagtcg
     4501 tccgggaagg ctgcaccatc ggctcgcggg tgatcattca tagcgggacg gtgatcggga
     4561 gcgatggttt cgggtacgtt caacatgagg gacgcaatta taaaatcccc caactgggtg
     4621 gggtcatcgt ggaagatgat gtggagctgg gcgccaatgt cgctgtggac cgggccacct
     4681 tcggccaaac cgtagtcaaa cgggggacca aggtagacaa cctggtgcag atcgcccaca
     4741 atgtcaccgt gggcgagcac accattctgg tggcccaggt gggcattgcc gggagcacgt
     4801 ccattggcca ccatgtcatg atcggcgggc aagccggcct cgcggaccat gtccgcgtgg
     4861 gggactcggc catgattgcc gcccgctccg gagtgaatcg gaacgtggcc agccaagaga
     4921 tcgtgggcgg cgccccggtg atgccctttg aagttttcct gaaatcccac gcccttcttc
     4981 cccgccttcc cgagttccgc cagcagctgc gggagctgaa agagcgggtc caggttttgg
     5041 aaaagaagga tgtcggccag gagaaaccca aaaagccgag acggaagccc tagtcccttt
     5101 tgggctcgct ttccttccca cgggattcaa gaatacttgc cccccgctgc atttctcaat
     5161 aaaaaacatg gccaaacatg aagggcgaac gagaggattt cgacgcgggg aattgggacg
     5221 cctcgggttg actctgacag caaagaatga atcggtcttt ggccaggcct aagggaaagg
     5281 agaaatcgta tcaaggcccg ctgtaggagt ccttttccgc aggggggaga agaagctctt
     5341 tatcgttctc cttggagatg tgggaccgct ccacattctc gatatactgt tctggaattt
     5401 tggccatcac ctcttcgatc gtggtaattc cttccctcac cttgccccag gcttcctcaa
     5461 agagggtttt catacccgat tcccgggccg catcccatag ctcgtcttcg tagacgttct
     5521 tggcaatcag cctgcggatc ttggtattga ttctcagaag ctcgaagacc ccgatctgtc
     5581 cgtagtaccc ggtgtaataa cagtggggac accctttccc ccgataaaag tgctctatgg
     5641 gaggataatc cttgtaaagg ctggcgttgg gcgggtccac ttcgaccttg catttcgggc
     5701 agatccggcg aaccaggcgc tgcgccagga ccccgctgac cgaggagctg accagaaaat
     5761 tggctacccc gatgtccacc agcctcatca ccgtagagac ggtatcattg gtgtggaggg
     5821 tgctcaaaac cagatgcccg gttaaggcag cgcggatggc aatctcggcg gtctccaggt
     5881 cccggacctc tccgatcatg ataatatccg ggtcctgacg aagaatggag cgaagggcgg
     5941 tggaaaaagt gtagcccacc gcctcgttga tcgaaacctg ggtgatcccc tcaaatttgt
     6001 attccaccgg gttttccaca gtgatgatgt tttccgtctc cgagcgcagc tggttcagcc
     6061 aggcgtagag ggtggtcgtc ttccctgcgc cggtggggcc ggtgaccagg agcattccct
     6121 ggggctggct gaagatctcg atcagctggt tgaaaatgaa ttcgggaatg cccaggtggg
     6181 ccaccgggat cagccccgtg ttgcggttca aaagacggat caccaccttc tccccataaa
     6241 tagagggcag ggtggagacc cgcaagtcca cctctttctc ccccaccttc aggtagctgt
     6301 ttccgtcctg cggaagcctg cggttggtga tgtccatatt ggagatgatc ttgatgcggg
     6361 agatgactgg gttttggacg ttcttgggga cccgcaggat gtcccggagt tctccgtcga
     6421 cccggtagcg gaccaggacg tacttctccc tcggctcgat atggatgtcg ctggctcggg
     6481 actgcaccgc gtcgacgatg atcatggtca ccagcttgac gatagccggc gcttcgctgg
     6541 acttgtagag ggactggacg ctgatctctt cctcggggga ttcctttttg aactccacct
     6601 cttcgaactt gggcatgtcc tttaccaggt cccagacgtt ctcttcggcc ccgtaattct
     6661 tttcgatggc ctccaggaga ttggtctcgc aggccaccac gtgcttgatg ttgaggttgt
     6721 tgatgaaagc cagctccccg atggtctcgt aatccagggg gtcggccatg gccaggagca
     6781 agctgcgccc ttggagttcc agcggaatga ccagcttctt ctccaccact tccttgggaa
     6841 cgagggattt gatctcttcg gaaatccgat attgggaaca gtccacgaag ggaagggaga
     6901 gctgcttggc cagagcttca gcgatctgcg tttcggagac gtaccccagt tcgaccagga
     6961 ttttgcccag acgcttgttc ttgctcttct gcagcatgag ggcgtgttca agctggtgcg
     7021 gggtgatcag cccggattcc atcaaaatgt cgctgattcg ttttctatgc ttgtacacgg
     7081 gactcctctg gggccatcca tccagggccc ccgcgccggt tgggtcagcg ggtaggttta
     7141 atccaatatt accaatacat ttctcttcgg gaattgtcaa ccccttttct tatcttgccc
     7201 accttcactt ttgcgagtct cttttctgct aaaaagcaag cttgtggccc ggaagcaagg
     7261 gcgttgcgcg gaaaaagcat cggcgggaaa agaaatccat tcggagatcc ccccttgctt
     7321 ccgcaaaatg aggc
//
LOCUS       DASPRR010000582        10128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_953447, whole genome shotgun sequence.
ACCESSION   DASPRR010000582 DASPRR010000000
VERSION     DASPRR010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953447"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(526..1692)
                     /locus_tag="VEL68_21350"
     CDS             complement(526..1692)
                     /locus_tag="VEL68_21350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXZ38590.1"
                     /translation="MQEINQRIDPEEIRNLLTRLIRSKSLNPPGDVRECAGIIAGALT
                     ARGLPAEIIEDKPGVANVVSHLNGERSGRTLIWNGHFDVVTPGEDWEVDPFGGEYKEG
                     FIYGRGTSDMKSGVAAMIIALGALKKAGAPFQGRIVFQAVGDEETGSDAGTRCMIRRK
                     IGAGADFAIVSEPTNLNVTIGNRGLRWLEVSVKGRASHAGRPHVGANTVHAAARIISE
                     LERLTFKAHHPLFEIPHPSLSVTMIQAGTKVNIIPERCTFSIDRRMMPGETSESISRE
                     IEEVLRKCSSDGIHSVVQITHEGWDPYAIDPSSPWVKGLCEAVEEVTGRFPQIKGKAG
                     CTDASHLYHHGKTPAVCFGPGLEDLAHKANERVALEKVVQAAKVYALSAAKLLK"
     gene            complement(1807..2301)
                     /gene="tpx"
                     /locus_tag="VEL68_21355"
     CDS             complement(1807..2301)
                     /gene="tpx"
                     /locus_tag="VEL68_21355"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HXZ38591.1"
                     /translation="MTFKGGPMTLLGTGVKVGEKAPDFKAVDNGMQLATLDSFRGKVK
                     IISSVPSLDTPVCDMETRRFNEEAGKLPDNVVVLTVSMDLPFAQKRWCAAAGVEKVKT
                     LSDYQSRSFGLAYGVVIKELQLLARAVFIVDDQDIVRYIEIVPEVTKEPDYDRALNAV
                     KALL"
     gene            complement(3044..5503)
                     /locus_tag="VEL68_21360"
     CDS             complement(3044..5503)
                     /locus_tag="VEL68_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38592.1"
                     /translation="MAFSKTPNSELREGDRSAGPAPTFLFLGIFLTSAATLTLEISII
                     RLLSIAQWHHFAFLVVSMALLGYGASGSFLSSFPSFLRRDPLGLLINGSGLFSLSALL
                     AYLAGNEIPFDLARIAFDRWQVFYLFLFYLVFSMPFFFSGLVISSALSQWSGTAGRLY
                     FSDLAGASLGCLLVLGLFRIFGGPGTVLFACFLGALASMMFGRMKKSRVVFPWMWAGF
                     LACLLFWQPSWMELRLSPYKGLSTALRFPGAHLLETRWSAASRVDVLRSPAARTAPGL
                     SLEYLGPLPEQLGLTVDGDDLTPITRLRGEPAGNEELKFLDFIPSAFPYKAFLPRKIL
                     ILEPGGGLEVLNALYHGTGEVVVVETNPAVAELLKTRYTEFAGNIYNRPEVRLIAEEG
                     RSFLHKSPSSFDLIVMPLSESLGASAGGMVGLQEDYRLTVEAFQEYLRALKPGGFLSF
                     SLYLLPPPRGELRLVSTIQRALEKGGNRPADHLLSFRTWGTFSLMVKKDPLDRREAEK
                     LKDFCRRMRFDLVYYPGISSEEANQFNRFPRPIYFDALQNLLREGESYISRYPFDLSP
                     ATDDRPYFHSFFRWKYWREVYRMAGGKWQILLEGGFLVPVVFTLALVLSALFLLLPAF
                     FRRGGGVKAAGNSLFAWLVCFTVLGFGFMFVEISLIQKFILFLGHPVYSVSLVIFSLL
                     VSAGMGSRFSLRFGDRSFRQLRIILLFLSGLLLLAAFSYPLILSFFQGNSSFVRWALT
                     FILISTPGFFMGMPFPLAVGLLGSMRPHWVSWGWCANGCASVLGAILPVLIALNWGFQ
                     AVFFLAALCYLVGFLVICKYG"
     gene            5661..6077
                     /locus_tag="VEL68_21365"
     CDS             5661..6077
                     /locus_tag="VEL68_21365"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HXZ38593.1"
                     /translation="MKRYETFDHTADMGIRVFGRTGEEVFANAAYALFDQLTDLRKVG
                     DRATQEISVEGADREDLLIRWLGELLFLSQSPGFLFKEFSILHLDSNCLRAIARGEIF
                     DPSRHRFKTEIKAVTYHQVEVKQENKGWEAKVIFDI"
     gene            6101..7552
                     /locus_tag="VEL68_21370"
     CDS             6101..7552
                     /locus_tag="VEL68_21370"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HXZ38594.1"
                     /translation="MSSQERIHLEKIDEVRWRIPRTGKMRVDGVIYTSEEMLRDINLD
                     ESPKQVANVAHLPGIQKYSLAMPDIHWGYGFPIGGVAAFDWKEGVVSPGGVGYDINCG
                     TRLLRSNLFRQDLRPRIKELADVLFESIPSGVGSKGALRLSSQELREVLKKGAAWAVE
                     NGYGSPEDLDRTEDQGCMEGADPGVVSDRAMERGRPQLGTLGSGNHFLEVGYVEEIYD
                     PEVARVFGLEKDQVTVFIHSGSRGFGYQVCDDFLKKMGERVRQLGLELPDRQLACDFI
                     HDRTGQEYLGGMACAANYAWANRQMLMHWTREVLQRLFRMSPRDLGMHLIYDVCHNIA
                     KREFHTVEGEKREVCVHRKGATRAFPPGHPAVPKVYREVGQPVLIPGDMGRYSYVLVG
                     TEKAMEETFGSACHGAGRVQSRTQAKKAGKGRPLHRELEDRGVYVRAASRTTLAEEAP
                     EAYKDVGQVVEVVHQAGISRKVAKLRPWAVVKG"
     gene            complement(7637..9763)
                     /locus_tag="VEL68_21375"
     CDS             complement(7637..9763)
                     /locus_tag="VEL68_21375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXZ38595.1"
                     /translation="MADEPKGMFDIRFWISDKKKTPMTEEKEIQKKSRKKRAWLTFLL
                     IILLVLVVLVGAGMAFYFSIVFDLPRLTTLKDYQPYITSEVYSEDEVLIGEFFIEKRT
                     VLSLSQMPRFLPKAFIAAEDARFFEHRGIDYWRILGAAFRNIEALDVVQGGSTITQQI
                     AKSFFLTPEKSYSRKLKEAILAQRIEHYLTKNEILFLYLNQIYLGEGAYGVAAAASTY
                     FGKPVQNLTLAECAILAGLPPSPNNLSPLRNPKKARERQLYVLSRMVERKIISKEQAQ
                     KAQAEEVRLRPRGPKGYHEAPFVMEQARQYVEQKYGKDLLYKGGLKIYTTINARLQKA
                     AEHAVQVGLEEFESRGGKGKGREAIQGALIALEPQSGFILAMVGGRDFSSSQFNRALQ
                     ARRQAGSAFKPIVYAAALDKGFTPATLIVDEPFSYEDVPGKDPWQPLNYDREFWGPIT
                     MRKALTFSRNVPTVRIAQSIGLDYLIDYAGNLGIKTKLEPNLSLALGAANVTLLELTR
                     AFGVFAAQGFRADPVLVSQIQDKDGNVLEEIEPSSLEVISPQTSYIITSLLQSVIQEG
                     TGQRARALGRPAAGKTGTTNDTRDAWFIGYLPQKLVAGAWVGYDVEKPLGTHETGAVA
                     ALPVWLAFMKEAIKGMPVQDFAAPEGVVFVKVDKENGEPLASMRWAKSGKVLFECFKE
                     GTEPTTGTQRPAGVQRQPDEEIPKTR"
     gene            complement(9802..>10128)
                     /locus_tag="VEL68_21380"
     CDS             complement(9802..>10128)
                     /locus_tag="VEL68_21380"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HXZ38596.1"
                     /translation="SQALQVGPRLVVNGRELQMKKQVARRSALGVTMKNQVILLTSED
                     TDVYAQDLARIFHLPESRGGLECRDAMALDGGPSTQMYAEYKDLKIDIPGGWGVPNGV
                     GVFKRK"
BASE COUNT         2301 a         2844 c         2731 g         2252 t
ORIGIN      
        1 aacaggatgt ttcttcgcat aattaagtat tttcttccgc tccattcaaa tcgctcctga
       61 atggcctgct aactgatctt agtcactttg gtctaaagct gatgcttgaa aatcttggtg
      121 ctggggcgag agagaaacca aattctcgat aaaaaggcaa aaaatacccg cctttcccga
      181 aaaaagacat tttcccacgg gtttgtggat tacctttgca aaaaatatac cttgaaaaaa
      241 atgtggattc aagtttcctg taattatttg acttttaagc ttaaatttta attttttagg
      301 gattctgaaa acgggatttc ccccctttaa gagaaatttt ttagccagat ctttacacta
      361 ttttgaccat agaaggaagt tttttgacct gggagaatag ctatggaaac aaactgtgag
      421 cacatcctgg atcccctgac ggtgctgggg actcagggga cgtggtggag ctgattcctt
      481 ttggttgcgg cactgccgcg ctgccacctc tgtggctgaa ggctttcatt tcagcagttt
      541 cgccgccgaa agggcgtaca cctttgccgc ttgtaccacc ttctccagcg ccactctttc
      601 attggccttg tgggccaggt cctccaaacc gggtccgaag cagaccgctg gagtctttcc
      661 atgatggtag aggtgggagg cgtcggtgca tcccgccttt cccttgatct gcggaaaccg
      721 ccccgtcacc tcctcgaccg cctcgcacag ccccttcacc caaggcgaac ttggatcgat
      781 ggcgtagggg tcccacccct cgtgggtgat ctggactaca ctgtggattc cgtcggaaga
      841 acatttccgt aagacctctt cgatctctcg ggagatggac tcggaagtct ctccgggcat
      901 catccgccgg tcgatggaaa aggtacaccg ctcgggaatg atgttcacct tggtccccgc
      961 ctggatcatg gtcaccgaca ggctgggatg gggaatctca aaaaggggat gatgagcctt
     1021 gaaggtcagg cgctccaatt cgctgatgat cctcgccgcg gcatggaccg tattggctcc
     1081 cacatggggc ctgccggcat ggctggcccg acccttgact gatacctcca gccagcgcag
     1141 cccgcggttc ccgatggtca cattcaggtt ggtgggttca gagacaatcg cgaaatccgc
     1201 cccggccccg attttcctcc ggatcatgca ccgggttccg gcatcgctcc cggtctcctc
     1261 atcgcccacc gcctggaaga cgattcggcc ttggaagggt gcaccagcct ttttcagggc
     1321 tcccagggcg atgatcatgg ctgccacacc ggacttcatg tcgctcgtcc cgcgaccgta
     1381 aatgaacccc tccttatact cccccccgaa ggggtctacc tcccagtcct cccctggagt
     1441 taccacgtcg aaatggccat tccagatcag ggtccttccg cttcgctccc cgtttagatg
     1501 ggaaaccacg ttggccactc cgggtttgtc ctcgatgatc tcagccggaa gcccccgggc
     1561 ggtaagggcc ccggctatga tccccgcgca ttcccgcacg tcccccgggg gattcaggct
     1621 cttggagcga atgagtcgag tgagcaaatt gcgaatctct tccggatcaa tcctttggtt
     1681 tatctcttgc acagcctcct cccctaaaaa gggagtcgag agggatcaag agtgagtagg
     1741 aaccaaaata ccctttgcct cttgcctcca acctctaacc ttctacctct acccgcttcc
     1801 gcttttctac aggagcgctt tcaccgcatt cagcgcacga tcataatccg gttccttggt
     1861 cacctcgggt acgatctcga tgtagcggac gatgtcctgg tcatccacga tgaagaccgc
     1921 tctggccaga agctgcaatt ccttgatgac cacgccgtaa gccagcccga aagatcggct
     1981 ttggtaatcg gaaagggttt tcactttttc cacccccgcc gcagcgcacc agcgcttctg
     2041 ggcaaaaggg aggtccatgc tgacggtgag aaccaccacg ttgtccggca gctttcccgc
     2101 ctcctcgttg aaccttcggg tttccatatc gcagaccggg gtatccaggg acgggaccga
     2161 gctgatgatc ttgaccttcc cgcgaaaaga gtccagagtg gccaactgca tcccattatc
     2221 caccgctttg aagtctgggg ctttttcccc cactttcacc cccgtcccca aaagggtcat
     2281 agggcccccc ttaaaggtaa tgagaccttt tctttcctcc gcgccggaaa ctgcctggga
     2341 tacataatct ttggccttgg actgcaattc ggcaattttc ttctccgctt ttctatggta
     2401 ctcctcgagc tcctttaatt tatcccctaa gggctgggct ttcttctcta gttcttcctt
     2461 caaccttttg tattcggctt ccgccttcgt cttccattcc atcagggtct ggtcggtctt
     2521 ttctttgatc gccgagacct ctttcttggc gctatccatc gcctctttaa gcgtcttttc
     2581 tccctggcgg atgaggcttt ccctatcggc catctccttt ttcagccttt ccaccaaatt
     2641 gtctttctgc tccgttagtt tttccgccct cctcttgact tccgcttctg ccttttcctt
     2701 ccattccagg agactctcct tggctttttc gaagaaactc tttttcgcct ctttgatggc
     2761 cttctctttc tccaccagct gtcccttcag cttcttgatt atttcctctt tttgagcaag
     2821 ggacttcttc catttgcttt ccagcttgcc cgcccccccg gccgcctttt tcaccagtct
     2881 ggaagtcttc cggctcactt tcttggccat cttcttcgcc tccttcttcg cctttttgat
     2941 cgctttcatg ctcaggccct cctcccttcc gatgtaggtg gtgagtagtt attgatcttg
     3001 ccccttgtct cttcccatgg atccttcctt cacttacccg atcttacccg tatttgcaga
     3061 ttaccaaaaa gccaactaaa tagcaaaggg cagccaggaa gaacaccgcc tgaaaccccc
     3121 agtttagagc aattaggaca ggtaagatgg ctcccaaaac agaagcgcag ccgttcgcac
     3181 accatcccca ggaaacccag tggggccgca tggagcccag gagcccgaca gccaggggaa
     3241 aaggcattcc catgaaaaat cccggggtcg agattaggat aaaggtaaga gcccagcgga
     3301 cgaatgaaga atttccctgg aagaacgata ggatcaaagg ataagaaaac gctgccagca
     3361 aaagaagacc ggacaggaaa aggagaataa ttcgcaactg ccggaaagaa cggtcaccga
     3421 accgtagcga gaaacggctt cccattcccg ccgagacgag gagggaaaaa ataaccaggg
     3481 aaacggaata aacgggatgg ccgagaaaaa gaataaattt ctgaatcagg gagatttcca
     3541 cgaacatgaa tccaaacccc aggacggtaa agcagaccag ccaggcgaag agggaattcc
     3601 cggcagcttt tacccctcct cctctcctaa aaaaggctgg aagaaggaga aaaagggcgc
     3661 tgaggacgag tgccagggtg aaaaccaccg ggaccaggaa tcctccttca aggagaatct
     3721 gccacttccc tccagccata cgatagacct cccgccaata tttccagcgg aaaaatgaat
     3781 ggaagtaggg gcgatcatcg gttgctgggc tcaaatcgaa agggtaccgg gaaatgtagc
     3841 tctctccctc tctcagcaaa ttctgcaagg catcgaaata aatgggccgc gggaaacgat
     3901 tgaactggtt tgcttcttcc gaggagatcc ctggatagta aaccagatca aaccgcatcc
     3961 gccggcagaa atccttgagt ttttcggcct ctcgccggtc cagggggtct tttttcacca
     4021 taagcgaaaa ggtcccccag gtgcgaaagg aaagaaggtg atcggcgggt cgatttccgc
     4081 ctttttccag agctctttgg attgtggaaa ccagacgcag ctctcccctg ggaggaggaa
     4141 ggaggtagag gctgaaagac agaaaaccgc ctggtttgag ggctcttaga tactcttgaa
     4201 aggcttccac ggtgagccgg taatcctcct gtagaccgac cattccaccg gcggaagctc
     4261 cgagactctc cgagaggggc ataacgatca aatcaaaaga ggacggagac ttatgaagga
     4321 aacttcttcc ctcttcggcg atcagccgaa cctcggggcg gttatatata ttccccgcaa
     4381 attccgtgta tcgggtcttt agcagttcgg caacagcggg gttggtttcc acaacgacga
     4441 cttctcccgt cccatggtac agggcattaa ggacttccag tccccccccg ggctccagaa
     4501 tcaggatttt tcggggtagg aaggccttat aaggaaaagc ggaaggaata aaatccagaa
     4561 atttcagttc ctcattccct gcaggttctc cccggagacg ggtgatgggg gtgagatcgt
     4621 ctccatccac tgtcaatcca agctgttccg gaaggggacc cagatattcc agactcaaac
     4681 cgggagctgt tctggctgcc gggctccgca ggacatccac ccgggaagca gcgctccagc
     4741 gagtttccag caaatgggca cccggaaacc gcagagcggt gctcagaccc ttataagggg
     4801 agagacggag ttccatccag gaaggctgcc agaataggag acaggccaga aagccggccc
     4861 acatccaggg gaagacaact ctcgatttct tcatccgccc gaacatcatc gaagccagtg
     4921 cgccgaggaa gcaggcaaaa aggaccgtcc ccggccctcc gaagattcgg aagagcccca
     4981 ggactagcag acaccccagg gaagcgccgg ccaggtcgga gaagtaaagc ctccccgctg
     5041 ttccgctcca ttggctcagg gcagaggaga taaccaggcc ggagaagaag aagggcatag
     5101 aaaagaccag gtaaaaaagg aagaggtaga agacctgcca ccgatcgaag gcgatccggg
     5161 ccaggtcaaa ggggatctcg ttccccgcca gataagccag aagggcgctc agggaaaaga
     5221 gcccgctccc attgatcaac aaccctaggg ggtcccgcct cagaaaggag ggaaacgagg
     5281 ataaaaaaga gccgctggcc ccatacccca gcagggccat actcaccacc aggaaagcaa
     5341 aatggtgcca ctgggcaatg gagagaaggc ggatgatgga aatttcaaga gtcagggtgg
     5401 cggcggaagt gaggaagatg cccagaaaaa gaaaggtcgg tgcggggccc gccgaccggt
     5461 cgccctcccg tagttcggag ttcggagtct tcgagaaggc cataggaaag gggtctttcg
     5521 ggatttcatc cccgaatggg caggaagcct tcaaagcctt tgaaacctgg ggtcggaaac
     5581 ggatttctta tttttgtatc ccggaagatg gataggatta taatatagtt ggaaaagcaa
     5641 tccaacttca aagttttcag atgaaaagat acgaaacctt tgaccatacg gcggatatgg
     5701 ggattcgtgt atttgggcgg acaggggagg aggtctttgc caatgccgcc tacgccctgt
     5761 ttgaccaact aaccgacctg agaaaggtcg gggaccgagc gactcaggaa atttccgtgg
     5821 aaggggcgga ccgggaagac cttctgatcc ggtggttggg tgagctcctg tttctttccc
     5881 agagcccagg attcctgttc aaggaattct ccattcttca cctagactcc aattgcttaa
     5941 gggcaattgc ccggggagaa atcttcgatc cttcccgcca tcgcttcaag accgaaatca
     6001 aggccgttac ctatcaccaa gtggaggtga agcaagaaaa caagggctgg gaagcaaagg
     6061 tcattttcga tatttgaatt cttggaatgg agggctgctc atgagcagtc aggagagaat
     6121 ccatctggaa aagatcgatg aggttcgctg gcggatcccc cgcaccggca agatgcgggt
     6181 ggacggagtc atctatactt ccgaggaaat gttaagggat ataaacctgg acgaaagtcc
     6241 gaagcaggtt gccaacgtgg ctcaccttcc gggaattcag aagtattccc tggccatgcc
     6301 ggacatccac tggggctatg ggtttcccat cgggggcgtg gctgcctttg actggaagga
     6361 aggggtggtc tcccccggcg gagtgggata tgatatcaac tgcggaacgc ggctgcttcg
     6421 ttcaaacctt ttcagacagg atttgcggcc caggatcaag gaactggccg atgtcttgtt
     6481 tgaatctatt ccgtcgggcg tgggttcgaa aggagccctg cggttgagtt cccaggaatt
     6541 gcgcgaggtc ctgaagaaag gggcggcctg ggctgtcgag aacggctacg gctcccctga
     6601 agacctggac cggaccgagg accagggctg tatggagggt gctgaccctg gagtggttag
     6661 cgaccgcgcg atggaaaggg gaaggcccca gctggggaca ctggggtcgg ggaaccattt
     6721 tctggaagtc gggtatgtag aagaaattta tgaccccgag gtggcccggg ttttcggcct
     6781 ggagaaggat caggtgacgg tcttcattca ttccgggtcc aggggattcg gctaccaggt
     6841 ttgcgatgat ttcctgaaaa agatgggcga gcgggtccgc caattgggac tggagctccc
     6901 cgaccggcag ctggcctgcg acttcatcca tgaccggaca ggacaggaat acctcggcgg
     6961 catggcctgc gcagccaatt atgcttgggc caaccggcaa atgctcatgc attggacccg
     7021 ggaggtcttg cagcggctat ttcggatgag cccccgtgac ctggggatgc atctgatcta
     7081 cgatgtctgc cataacatcg ccaagcggga atttcataca gtggagggag agaaacgtga
     7141 ggtctgcgtc caccgcaaag gggccacccg ggccttcccc cccggccatc cggcggtccc
     7201 caaagtctac cgggaagtcg ggcagcccgt tctcattccg ggagacatgg ggaggtattc
     7261 ctacgtgctg gtgggaacgg agaaagccat ggaagaaacc ttcggcagcg cttgccacgg
     7321 cgctggaaga gtccaaagcc gcacgcaggc aaaaaaggcc ggcaagggaa ggcccctcca
     7381 ccgggagctg gaggatcgtg gggtctatgt tcgcgcagcc agccggacca cgctcgcgga
     7441 ggaagcgccg gaagcatata aagacgtagg ccaggtcgtg gaggtcgtcc accaagcggg
     7501 aatctcccgc aaggtggcca agttacggcc atgggccgtg gtgaaaggct gacacagtaa
     7561 gaaactgacg cggggatggg gagacgcggg aaagcggaga aaaaaatgag tcctttctcc
     7621 gtgtccccgc gtcaccctat ctcgtctttg ggatctcttc gtcgggctgc ctctgtaccc
     7681 ccgcaggccg ctgagtcccg gtggtaggtt ccgttccctc cttgaagcac tcaaacagga
     7741 ccttccccga tttggcccaa cgcatggagg ccaatggttc gccgttttcc ttgtccactt
     7801 tgacgaaaac gaccccctcg ggagcggcaa aatcctgcac cggcataccc ttgatggctt
     7861 ccttcatgaa cgccagccag acgggcaggg cagccacagc tccagtctca tgggttccca
     7921 ggggtttttc gacgtcgtaa ccaacccagg ctccggcaac aagcttttgg ggcagatacc
     7981 cgataaacca ggcatcgcgg gtatcattgg tcgttcctgt cttcccagca gccggacggc
     8041 ccagggccct ggcccgctgt ccggttcctt cctgaatcac gctctgcaga aggctggtaa
     8101 ttatatagga ggtctgcggg ctgatcactt ctagagagga aggttcgatc tcctccaaca
     8161 catttccgtc cttgtcctgg atctgcgaga ccagaaccgg gtcggcccgg aatccttggg
     8221 ctgcaaaaac cccaaaggcc cgggttaact ccaggagggt cacgttggcc gcccccaagg
     8281 ccagggagag attgggttcg agcttggtct taatccccag atttccagca tagtcaatca
     8341 ggtagtccag gccgatggac tgggcaatcc ggacggtggg aacgttccgg gaaaaagtca
     8401 gggccttgcg catggtaatc ggcccccaga attcgcggtc gtaattcagg ggctgccacg
     8461 ggtcttttcc tggcacgtcc tcataggaga agggctcatc gacgatcaaa gttgcagggg
     8521 taaaaccctt atcaagggct gcggcgtaaa cgatgggttt gaaggccgaa ccggcctgtc
     8581 tcctggcctg aagggcccgg ttaaattggc tcgatgagaa gtcccgtcca ccaaccatcg
     8641 ccagaataaa tcccgactgg ggttcaaggg cgatcaaggc cccctggatc gcttcccttc
     8701 ctttcccttt gcctccccgg ctttcaaatt cctccagtcc cacctgcacc gcatgctccg
     8761 ccgccttctg cagcctggca ttgatcgtgg tgtagatctt caggccccct ttatacaata
     8821 aatcctttcc atatttctgc tccacatact ggcgggcctg ttccatcaca aagggggcct
     8881 cgtgatatcc cttgggccct cggggcctca gcctgacttc ttctgcctgg gccttctgcg
     8941 cctgctcctt gctgatgatc ttcctttcta ccatccggct gagcacgtac agttgacgtt
     9001 cccgggcttt tttgggattg cgcaggggag aaaggttgtt cggggaaggg ggaagcccag
     9061 caaggatggc acactccgcc aaggtcaggt tctgtacagg tttcccaaaa taggtactgg
     9121 ccgcggcagc caccccataa gctccctccc ccaggtaaat ctgattcaag tagaggaaga
     9181 ggatctcatt cttggtcaag tagtgctcaa tgcgctgggc caaaatggct tcctttaatt
     9241 ttcgggagta actcttttcc ggagtgagaa agaaggattt ggcaatctgc tgggtaatcg
     9301 tgcttcctcc ctgaaccaca tccaaggcct caatattccg gaaggctgca cccaaaatcc
     9361 tccagtaatc gatcccccgg tgttcaaaga accgggcatc ttcggcagcg atgaaagcct
     9421 tgggcaggaa tcggggcatt tgagaaagag agagcaccgt cctcttctcg ataaaaaact
     9481 ccccaatcaa tacctcatcc tctgagtaga cttcggaggt aatgtagggc tgatagtcct
     9541 tcagggtcgt aagccgggga aggtcgaaga cgatagaaaa gtaaaaggcc atcccagctc
     9601 ccaccagaac cacgagcacc agaaggatga tcagaagaaa ggtaagccat gccctctttt
     9661 tcctgctctt cttctgtatt tctttctctt cggtcatcgg cgtttttttc ttatccgaaa
     9721 tccaaaatcg aatatcgaac attcctttgg gttcatcggc catcggtcat ctgctcttcg
     9781 tcaatcctca gaagtctgct gctactttct cttgaatact ccgaccccgt tgggaactcc
     9841 ccatcccccg gggatgtcga tctttaaatc cttatactcg gcgtacattt gcgttgaagg
     9901 gcccccgtcc agggccatgg cgtcccggca ctccagtccg ccccgggatt cgggaaggtg
     9961 aaagattcgg gccaagtcct gggcgtagac atctgtgtcc tccgaggtga ggaggatgac
    10021 ttggttcttc atggtcacgc ccaaggccga ccgcctggcg acctgctttt tcatctggag
    10081 ttcccgtcca ttaaccacca gccgggggcc cacctgtaaa gcctggga
//
LOCUS       DASPRR010000583         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1271028, whole genome shotgun sequence.
ACCESSION   DASPRR010000583 DASPRR010000000
VERSION     DASPRR010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271028"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VEL68_21385"
     CDS             complement(<1..321)
                     /locus_tag="VEL68_21385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ38597.1"
                     /translation="MWVPISIGFALEEAARLYGDNEFLVVNENRMTYRELSQGVRKFS
                     RGLLAAGVKAGDHVAVWLPNGIEWVVAVFSLANLGAVFVPVNTRFKAEELEYILKQSE
                     ASALI"
     gene            568..1620
                     /locus_tag="VEL68_21390"
     CDS             568..1620
                     /locus_tag="VEL68_21390"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017221940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXZ38598.1"
                     /translation="MIKTLMTEMFGIRYPIMLAGMNWISTPKLVAAVCNAGGLGTLAI
                     AQYSPEDTRKQIRQIRELTDKPFMINQPLHRGWAKENIQAAIEEKVPFINYSLGKPWF
                     TDQVHAYGGKVVGTIAIAKHAARAAELGCDALIVTGNEAAAHGADATSLVLIPFVTSM
                     VKIPVIAAGGFYDGRGLAAALSLGAAGISMGTRFILTQESGVHDNFKQLCLKATEQDT
                     LYSSAFDGMPGRALKTSIAEKIARGDSFSLLKAIPSALEIKKILKQSWWQFLGTAWKM
                     MAGEGGDLVQMARLANGTMRHQKAIYEGDTKEGFMFVGQVTGPIRDLPTVKELIDRMM
                     VEAEAVLQKTGRMVVS"
     gene            1809..2504
                     /locus_tag="VEL68_21395"
     CDS             1809..2504
                     /locus_tag="VEL68_21395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXZ38599.1"
                     /translation="MMKKEGKRLFAKTGSSNPLDQKIYAYLTEKISEGALSGGDRVVE
                     AELQKVFKVSRTPIREALRILTSRGLLQMVPGKGASIRLVSFEDIKEIYPVLANLEGL
                     AANMASDQIVEETLAEMKSCLEQMESLNQAGEKRPYIEYHYRFHELYIQSCQNQHLIQ
                     IIMDLRRQVHLYRLSHLYIPMAHSYSMTIHEKILRLLREKNKKEVEELVKEHMLIAMD
                     RFLSSKPGTQGQD"
     gene            complement(2537..3271)
                     /locus_tag="VEL68_21400"
     CDS             complement(2537..3271)
                     /locus_tag="VEL68_21400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ38600.1"
                     /translation="MTEKDIMTEEEGGILKIVLNRPRVRNALTISMMDEITFHLESRE
                     NSPALRVVIFSGAGGSFCSGSDFRQLQPDQGAEKIRQHLKAIADMYKALWKTSKFLIA
                     EVHGNALGGGMALALGCDWIIASAGARFGLPEMKLGFLPALALVPLAHRVHGRALELM
                     MTGEILEAAQAQALGLVSQVLGEDRVSSRVGDLARLLVSGDPESSAIAKRVYRTLLSM
                     DYEQGVDYAAGVILARLCGEKRPGGK"
     gene            3468..3923
                     /locus_tag="VEL68_21405"
     CDS             3468..3923
                     /locus_tag="VEL68_21405"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ38601.1"
                     /translation="MGWRKPDYKSGIFFLLLGLWVCYFSWKLGLGSPAKPGPGFMPFL
                     AGLLMAFLSFLLVIQVLFSWTASTWATGVQWKNTLLVLASMAAYGFLLDKIGFVLVTL
                     GFVILLMKVIEPQSWKRAVLGGIISSMASYLLFETLLKSQLPRSFLGFF"
     gene            3931..>5034
                     /locus_tag="VEL68_21410"
     CDS             3931..>5034
                     /locus_tag="VEL68_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ38602.1"
                     /translation="MDYYSLILQGFSIVFQPTNLFLCFFGCLMGTLVGVLPGLGPTAA
                     IALLLPTTFHFAPVSAIITLSGIYYGAMYGGSTTSILVNIPGEAASVITTLDGYQMAL
                     DGRAGPALGIAAFGSYIAGTLSIVGLMFLAPLLARGALAFGPPEYFSLMCMGLTILIY
                     LASGSMIKALMMAALGLIIGCIGADMLTNVHRFTLGIPELVDGVGLIPLVMGLFGISE
                     VLLNIEIQVRQEILKGKIKNLLPNLQEWKDSAMPILRGSLLGFLLGILPGSGPAIASF
                     VSYAVEKRVSRHPENFGRGAIEGVAGPESANNAATGGSFIPLFSLGIPSNSVMAILLG
                     ALMIHGVIPGPNLMQQSPDLFWGVVASMYVGNIM"
BASE COUNT         1085 a         1426 c         1341 g         1182 t
ORIGIN      
        1 aatcagagca gaggcttccg actgtttgag gatgtattcc agctcctccg ccttgaatcg
       61 ggtattcacc gggacgaaga cggctcccag attggccagg gagaaaaccg ccaccaccca
      121 ttcgatcccg ttgggcagcc agacggccac atgatctccg gctttcaccc ccgccgccag
      181 aagtccccgg gagaatttcc ggaccccctg ggagagttcc cggtaggtca tgcgattctc
      241 attgaccacc agaaactcgt tgtctccgta gagacgagcg gcttcttcaa gcgcgaaacc
      301 gatcgatatg ggaacccaca tgcccaaggc cttttcctta ctgcctatcc ttttttgtat
      361 acatttcaat gactgagatc agaaaatgtg tataggatta tacgggccgg gagaaggcct
      421 gtcaagaagt tttccggcat tttcccgggt tcttttcaga ggtttccgtt gcttttcttt
      481 tgaattttta gtatacctaa ggcctgctgg atgaagatga ggatattcat cttcttttcc
      541 ccatccaaca acctctggga ggctgacatg atcaaaacct taatgactga gatgttcggg
      601 attcggtacc cgattatgct ggcggggatg aactggatct ccaccccaaa gctggtggcc
      661 gcagtgtgca atgcgggggg tttgggcacg ctagccatcg cccaatacag cccggaggat
      721 accaggaaac agattcgcca gattcgggaa ttgacggata aacccttcat gatcaaccaa
      781 cccctccatc gcgggtgggc caaggagaac atccaggcag ccatcgagga aaaggttccc
      841 tttatcaact attcactcgg aaaaccctgg ttcaccgatc aggtccatgc ctacggaggg
      901 aaagtcgtcg gcaccatcgc catcgccaag cacgccgccc gcgccgccga actggggtgc
      961 gacgctttga tcgtgaccgg caacgaggcg gccgctcatg gagccgatgc cacctctctg
     1021 gtattgatcc cttttgttac cagtatggta aagattccgg ttatcgccgc cgggggtttt
     1081 tacgacggcc gcgggctggc ggcggccctt tcccttgggg ctgcgggaat ttccatgggg
     1141 acccggttca tccttaccca ggagagcggg gttcacgaca acttcaagca gctttgtctg
     1201 aaggccacgg agcaggatac actttacagc agtgcatttg acggcatgcc gggaagagcc
     1261 ctcaaaacct ccatcgcgga aaaaatcgcc aggggagact ccttcagcct ccttaaagcc
     1321 atccccagcg ccctggagat caagaagatt ttgaagcaga gttggtggca attcctggga
     1381 accgcctgga agatgatggc cggcgaggga ggagacctgg tccaaatggc ccgtctggcg
     1441 aacgggacga tgcgtcacca gaaagccatc tatgaagggg ataccaagga agggttcatg
     1501 ttcgtcgggc aagtgacggg acccattcgg gaccttccta ccgtgaagga gctgattgac
     1561 cggatgatgg ttgaagcgga ggcggtcttg caaaaaacag gaaggatggt tgtctcctag
     1621 acggggacga caggcagagt tcttcatcaa atccctcacc ggagtttctt tcttaggcgc
     1681 aacttcttta tacattttta ttgattgagc gtatcatttg gtatacatta attttagggt
     1741 ttctgagttc cgcccttttg cgaaatcctc cttctcagga gccataggac ttcataaaag
     1801 gattctggat gatgaaaaag gaaggaaaaa ggctcttcgc caaaacggga tcctctaatc
     1861 cccttgacca gaagatttac gcttacctca cggagaaaat atctgaggga gctctttctg
     1921 gtggcgaccg ggtggttgag gcggagcttc agaaggtgtt caaggttagc cggacaccca
     1981 tccgggaagc cctgcgcatc ctgacttcgc ggggcctttt acaaatggtc ccgggtaagg
     2041 gtgcctccat acgcctggta tcctttgaag acatcaagga gatttatccc gttctggcca
     2101 atctggaggg tctcgctgcc aatatggcct cggatcaaat cgtcgaagaa actcttgccg
     2161 aaatgaaatc ctgcctagaa caaatggaga gcctgaatca ggcgggggaa aagcgaccct
     2221 atatcgagta ccattaccgc tttcacgagc tttacatcca gagctgccaa aaccagcatc
     2281 tcattcagat catcatggac ctgcgccggc aggtccacct ctaccgtcta tcccacctct
     2341 atattcccat ggcccatagt tactccatga ccatccacga aaaaatcctc cgtctcctcc
     2401 gggaaaaaaa caaaaaggag gttgaagaac tggtcaagga gcacatgctc atagccatgg
     2461 accgcttcct cagttcaaag cctgggaccc agggtcaaga ctaaatccga cccggcgaaa
     2521 ccgatctccc gatcgcctat ttcccccctg gcctcttctc accgcaaagg cgggccagga
     2581 tgaccccggc ggcataatca actccctgtt catagtccat ggaaagaagg gtacggtaaa
     2641 cccgtttggc tatggctgaa gattcagggt ctcccgaaac cagcaagcga gccagatcgc
     2701 cgactcttga ggagacccga tcttccccca gcacttgcga gaccaggccc agagcctggg
     2761 cctgtgcggc ttccagaatc tccccggtca tcatcaactc caacgcccgc ccgtggaccc
     2821 ggtgagccaa gggaacgagg gccagggcgg gcaagaatcc gagtttcatc tccggcagtc
     2881 cgaagcgcgc accggcggag gcaatgatcc agtcgcaccc caaagccagg gccattcccc
     2941 ctccaagagc atttccgtga acctcggcga tcaaaaattt tgaggtcttc cagagggctt
     3001 tgtacatatc cgcaatcgct ttgagatgtt ggcggatctt ttcggcccct tgatccggct
     3061 ggagttggcg aaaatccgag cccgagcaga aggaacctcc ggcgcccgag aagatgacca
     3121 cccggagggc gggagaattt tcccggcttt ccaggtggaa ggtaatctca tccatcatgg
     3181 atatggtcag ggcatttcgc acccggggcc ggttgagcac gatctttaaa attccccctt
     3241 cctcttccgt cataatatcc ttctctgtca tggggcttcc tctctctgcc caaaaaatgt
     3301 atacaccctg tgcaggcctt tccgtaatta tcgttgacaa tataccctat attcagtata
     3361 cagaaaacac gaattccagg gtagccattc gaacccatcc gtttttggcc ctaacgaggg
     3421 atcttgaatc ccgattccga aaagcaggga gaaggaattt ggggcgattg ggctggagaa
     3481 aaccggacta taaaagcggg atattctttc tcctcctggg gctttgggtc tgttatttct
     3541 cctggaagct cgggctcggc agtccggcca aacccgggcc ggggttcatg cctttcctgg
     3601 ccgggctcct catggcgttt ctttctttcc tgttggtgat tcaggttctc ttcagctgga
     3661 ctgcttccac ttgggcgacg ggggtccagt ggaaaaatac tcttcttgtc ctggcgtcca
     3721 tggcggccta tggattctta ctggacaaga tcgggtttgt cctggtgact ttgggcttcg
     3781 taatcctcct gatgaaagtc atcgaaccgc agagctggaa gagggccgtc ctgggaggga
     3841 tcatttcttc tatggcctcc tacctcttgt ttgaaaccct gctgaagagc cagcttcccc
     3901 ggtctttcct ggggtttttc tgagggaagc atggattatt attctctgat cctccagggt
     3961 ttttcgattg tcttccaacc gaccaatctc tttctctgct tcttcggctg cctgatgggg
     4021 actctggtag gagtcctccc ggggttaggt cccactgcgg ccatcgccct tctccttccc
     4081 acgaccttcc attttgctcc ggtctcggcc atcatcaccc tctccggcat ttattacggc
     4141 gccatgtacg ggggttcgac cacctcgatt ctggtgaaca ttccgggaga agccgcgtcg
     4201 gtcatcacca ccctcgacgg ataccagatg gccctcgatg gacgggccgg gccggccctg
     4261 ggtattgccg ccttcgggtc ttacatcgcc ggaacgttga gcatcgtggg gctcatgttt
     4321 cttgcccccc tcctggcccg aggcgccctg gccttcggtc ccccggaata tttttctcta
     4381 atgtgcatgg gcctcacgat ccttatctat ctggccagcg ggtccatgat caaagccctg
     4441 atgatggccg cgctgggttt gatcatcggc tgcatcgggg cagacatgtt gaccaacgtt
     4501 catcgcttta ccctgggcat tcccgagttg gtggacggag tgggactcat tcccctggtc
     4561 atgggtctct tcggaatctc cgaggtcctg ctcaacattg agattcaggt caggcaggaa
     4621 atcctcaagg ggaagatcaa gaacctcctg cccaatcttc aggagtggaa agacagcgcg
     4681 atgccgatcc tccgcggctc cctcttgggg ttcctcctgg gtatcctccc cgggtccggc
     4741 ccggccatcg cttcttttgt atcctacgca gtcgagaagc gggtttcccg ccaccccgaa
     4801 aatttcggcc ggggggcgat tgaaggggtc gccgggccgg aatcagccaa caatgcggcc
     4861 acgggcgggt ctttcatccc cttgttcagt ctcggaattc cttcgaattc ggtcatggcc
     4921 attcttctgg gcgccttgat gattcatgga gttattcccg gacccaacct gatgcagcag
     4981 agcccggacc tcttctgggg agtggtggcc agcatgtatg tgggaaatat catg
//
LOCUS       DASPRR010000584        11425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1914206, whole genome shotgun sequence.
ACCESSION   DASPRR010000584 DASPRR010000000
VERSION     DASPRR010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914206"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VEL68_21415"
     CDS             complement(<1..298)
                     /locus_tag="VEL68_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38603.1"
                     /translation="MHKNLFLLTLSGFLMIFFGGAALAQHQHDHGATPKPATKPLEKS
                     AEKGSIQSVTVEGLKVSLQVMDMTAHMNMPGMKGNPQHGESHSQSHALMVTIQDT"
     gene            complement(655..1272)
                     /gene="metW"
                     /locus_tag="VEL68_21420"
     CDS             complement(655..1272)
                     /gene="metW"
                     /locus_tag="VEL68_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HXZ38604.1"
                     /translation="MDTTWPRVMISNGIRLDHQIICRIVELGARVLDLGCGNGDLLYL
                     LAKEKGAKVQGIELNDSAIYECVKKGLSVFHGDIESGLAEYPDQSFDYVILNQSLQEV
                     KKIDGLIHEGLRIGGKIIVGFPNFAYLWARWMLFFRGKAPITPSLPYRWYDTPNLRFL
                     SVSDFRDFCREKNLKVKEAHYLGKKGRILYWANLFALNAIFVVTK"
     gene            complement(1238..2389)
                     /locus_tag="VEL68_21425"
     CDS             complement(1238..2389)
                     /locus_tag="VEL68_21425"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HXZ38605.1"
                     /translation="MNKNGSVGVVKTKYFTFAEPSGPLVLESGRVLSPVTLAYETYGT
                     LNRDKTNAILVAHALSGDAHAAGFHKGESGPGWWDVMIGPGKAFDTDRYFVICSNVIG
                     GCKGSTGPSSINPLTGKPYGLDFPVITIADMVEAQRHLVDHLGIEKLLCVTGGSMGGM
                     QALQWVASYGERVRSAIPIAAALKHSPQQIAFDEVVRQSIMADPAWRQGEYYEHGQPE
                     KGLAVARMIGHITFMSDQSMEEKFSRKLKNGQYNFDFAADFEVEGYLRNRGDHFVRRF
                     DANSYLYITKAMDYFDLSRGRLIPNGKVLSTRFLVISFKSDWLYPSYQSQEIARELKM
                     KRADATYCELPSTYGHDAFLLEVEEQTTLIRHFLDKTLNGYHVAESNDI"
     gene            complement(2482..3780)
                     /locus_tag="VEL68_21430"
     CDS             complement(2482..3780)
                     /locus_tag="VEL68_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HXZ38606.1"
                     /translation="MSKNDANLRIETLALHGGQEADPTTGSRAVPIYQTTSYQFKDTD
                     HAANLFALKEFGNIYTRIMNPTNDILEKRVALLDGGVGALAVASGQAAETLALLNIAR
                     AGDEIVSTDNLYGGTYNLFHYTFPRLGIEVKFVPSNDLEAIHKAITPKTKAVYAESIG
                     NPKLDVADLEGLSRVAHQHGIPLVLDNTVSPYLMRPIDFGVDIIVYSATKFIGGHGTS
                     IGGLIVDSGKFDWTNGKFPLIADADPSYHGLKFVEALKPLGNLAYIIKARVTLLRDLG
                     PAMSPFNAFLFLQGLETIHLRLPRHSENALAVAQYLEKHARVAWVNYPGLASSPEKGR
                     VKKYLPKGAGAILGFGIKGGIEAGRKFINSLELVSHLANVGDAKTLAIHPATTTHQQL
                     SPEEQLATGVTPDFIRLSVGLEHIDDILKDIEQALKKTGK"
     regulatory      complement(3969..4082)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4130..4600)
                     /locus_tag="VEL68_21435"
     CDS             complement(4130..4600)
                     /locus_tag="VEL68_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXZ38607.1"
                     /translation="MDSREFSSLRQRLQKTQKQMAQILGTSLKAVQSFEQGWRKVPVH
                     IERQVLFLISNWGEGLRKSRPCWDIRDCSTKNREACPAWEFNLGHLCWFVNGTICRGE
                     IQGGWPQKMKLCRTCEVFIQTVRPGNLVEEGRSRVRREGKEGKKKVGRGRALIG"
     gene            4715..5014
                     /locus_tag="VEL68_21440"
     CDS             4715..5014
                     /locus_tag="VEL68_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HXZ38608.1"
                     /translation="MGGISAAIERDIPKNREGRFPRFDRELNLKGKICPYTFIESMLT
                     LEEMKRDEVLRVIVDYPPAVCDVPRSLKNEGYEVLDVSPINETDWEIWVRNKEVE"
     gene            5021..5821
                     /gene="moeB"
                     /locus_tag="VEL68_21445"
     CDS             5021..5821
                     /gene="moeB"
                     /locus_tag="VEL68_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXZ38609.1"
                     /translation="MKFSEEQIHRYSRQMILPGIGGKGQRKLGEARVFIMGAGGLGSP
                     AAIYLAAAGVGAIGLADFDRVELHNLQRQILHRTPDIGLLKVDSGKKTLETLNPEVQV
                     QVYSERITSANIRDIIKDYHLVLDGSDNFPTRFLVNDACFFEKKTLISGAILRFDGQL
                     STFKPHAGGPCYRCLFPEPPPPGTIPSCQEAGILGAVAGTIGLLQANEALKEILGLGR
                     SMSGRLLLFNALSLCFDEVKIEPNPVCRLCGKNPAIKELIDYPLTCQA"
     gene            6038..7129
                     /locus_tag="VEL68_21450"
     CDS             6038..7129
                     /locus_tag="VEL68_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXZ38610.1"
                     /translation="MLRRRIKYFLFLAFFSCLTGFCGQGQVQALTDDERNNVTIYQKA
                     SPSVVNVISTVITRDFFLNAIPREGSGSGSIVDARGHVLTNNHVIRDAKKLEVTLADS
                     SKWPAKLVGTDPDNDLAVIKIDAPADKLKPLPLGDSQKLQVGQKVLAIGNPFGLGLTL
                     TTGIISSLGRNIRSEVGTMIEDLIQTDASINPGNSGGPLLNSEGEIIGINTAIISPTG
                     ASVGIGFAIPVNTAKRIIPQLIAKGYVIYPWAGASVQPLFPELAQVLKLKVERGAMVI
                     EVTQGGPAEKAGLRGGNRQIQVGNVVIIVGGDVITRIDQSEVKDADDLIKHIRERKPG
                     EVVILKVLRDGQFKEVKITLQERPRATGR"
     gene            complement(7251..8003)
                     /locus_tag="VEL68_21455"
     CDS             complement(7251..8003)
                     /locus_tag="VEL68_21455"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXZ38611.1"
                     /translation="MDLHLKGKVAVVTGGASGIGEKTAEFFVQEGVKVLVADVNAERI
                     ENTVGSLKRGKGEAKGISCDVTQEEQVSRMVREALSHFGTIDFLVNSAGIGGGMNPLE
                     KAPRGEWQRILDVNLIGTILCCRAVMPILREKKIGRIINIASEAGVSGEKGLEVYAAS
                     KGGVISLTKSLAKNLGRDKITVNAVAPAFVHSPMTAYLTPEIEQKWLKMYTVKRLGET
                     GDVASMIVFLCSDRCSWITGQTISINGGFSMK"
     gene            8298..9035
                     /locus_tag="VEL68_21460"
     CDS             8298..9035
                     /locus_tag="VEL68_21460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXZ38612.1"
                     /translation="MEFRSKIPRTLPIRQKGFTLTEVLFVLAILGVLLAVAGLFYSGY
                     RTKVSETVAQEDLRQAYNSAMAFFIDFPQRALTRVDLEKYGFRSSARIETRVVDGRLD
                     HLLLVSYYTLPGSKIFMIEGLRGDFTGGKEPSGPIIGPGGSGGGGSFSDPLIQPMNEM
                     GWGRCNDKAKTELKEAFNAAQKYFAKDPGGSITKDILPDYGYLPSEEVNLLVINGKKP
                     SLEISAIFSVPGASNFMIDSSGFIREG"
     gene            9225..10355
                     /locus_tag="VEL68_21465"
     CDS             9225..10355
                     /locus_tag="VEL68_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017376001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HXZ38613.1"
                     /translation="MGNPKDFIGTSKWNLDTPCLVIDQDILEQNIGNMQAHLTSFGKK
                     LRPHAKTHKCSIIAKKQVAAGGIGICVAKVSEAEVMVKAGMRGVLITSPVVTDQKIVR
                     LMECLFRDPDILVVVDNPTNARMLNEAARKQGGRLSVLVDLDPGMGRTGVSFREGLAL
                     GQWIHSLPAMRLRGIQCYAGNVQHILGFQERKEASLGWLGQAAEVFRQFQQNRLPTEV
                     FTGSGTGTYEIDCQVRELTDLQAGSYILMDTEYLQIGCSQNPNKFEKFPPALTLLTTV
                     ISSNHPGFFTVDAGLKALYHHGGTPSVLHPEGLGLVYEWRGDEHGKISLPAPGRKFEI
                     GEVLELVVSHCDPTVNLFDFFYVTRAGTVVDVWPIDLRGKGQ"
     gene            complement(10387..10980)
                     /locus_tag="VEL68_21470"
     CDS             complement(10387..10980)
                     /locus_tag="VEL68_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38614.1"
                     /translation="MRELFVGLAILLTLTSTPPALADYLIKLKNGRTLETPRFWEERG
                     EIKFQWLGGVVSLSKKDLFTITEVENKFADQTSTREKQRAPENPVTPPGAPLATMNIV
                     EKVLPAERLSSAETLQGVGMEDYRKKKTYYIEQFEQAYQRYLEASSRKDVEGKKKAWE
                     EFNRFGGQVITLETELKKKNNGTLPSWWKEEKNKEVK"
     gene            complement(11036..>11425)
                     /locus_tag="VEL68_21475"
     CDS             complement(11036..>11425)
                     /locus_tag="VEL68_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38615.1"
                     /translation="HVNSATLPGMDWGSPIPGATQRDPSDCSLRNIVYFNTNGTYVRL
                     SGGSHGCGTLLVEGDLCVYGAFQWYGVILVTGSIIFSGGSGKNVTGAIMAGGMVSADL
                     MGGGANIIFCSRAVQDQTIYLPLVTLR"
BASE COUNT         2555 a         3143 c         2982 g         2745 t
ORIGIN      
        1 ccgtatcctg gatggttacc atcagcgcgt gactctggga atgactctcg ccatgctggg
       61 ggtttccctt catgcctggc atgttcatat gggcggtcat atccatgact tggagagaaa
      121 ccttaagccc ctcgacggtc acggattgaa tgcttccctt ttcagcagat ttctccaagg
      181 gttttgtagc tggtttgggg gtcgcgccgt ggtcatgttg atgctgggcc aaggcggcgc
      241 ccccaaagaa aatcatcaag aatcctgata aggttaataa aaacaaattc ttatgcatct
      301 gcaaatcctc cttctgtact tactctgtat ttttgattct catttaattt tagatgctta
      361 ctttttatga gtaaattaat ttattatata acctgcgatt tctcccgtag tatggggtaa
      421 atttcctatt tttaagggga aaaaacagta ggggcgggat atttgatgag gacctagagg
      481 gttatggccg tggccatgat gatcagcgtc gggagataaa gagccttata agttcgacct
      541 ttttttgatt ccgaaacctt gatcacagac ctttgaaaat ggataacccc atccccccta
      601 ggatcatttt tataccccgg gcaaccaaac ttcggaatgt cttgactttc ttggctattt
      661 ggttaccacg aagatcgcat tcagggcgaa aaggttcgcc caatagagaa ttctgccttt
      721 ctttcccagg tagtgggctt cctttacctt aagattcttc tcccggcaaa aatccctgaa
      781 gtcgctgacg ctcaagaaac gcaggttagg ggtatcatac cagcgatagg gaagcgaggg
      841 ggtgatgggt gccttccccc tgaaaaacag catccaccgg gcccagaggt aggcaaaatt
      901 gggaaatccg acgatgattt tacctccgat gcgcagcccc tcatggatga gcccgtcgat
      961 tttcttcact tcctgcaagc tctggttcag gataacataa tcaaaagact ggtcgggata
     1021 ctccgccaaa ccgctctcaa tatctccgtg aaagacactc agtccctttt tcacgcactc
     1081 atagatggcc gaatcgttca gctcgatccc ctgaacttta gctccttttt cctttgcgag
     1141 aaggtagaga aggtccccgt tcccgcagcc caggtccaaa acccttgctc ccaattcaac
     1201 gatccgacaa atgatttgat gatcgagtcg aattccgtta gatatcatta ctctcggcca
     1261 cgtggtatcc atttaaggtt ttatccagga agtgccggat cagagtcgtc tgctcttcca
     1321 cttccagcaa aaaggcgtcg tgtccgtaag tcgagggcaa ttcgcaatac gtagcgtcgg
     1381 ctcgcttcat cttcagctcc cgggcgatct cctgggactg gtaggatggg tagagccaat
     1441 ccgatttgaa ggagatcacc agaaagcggg tggagaggac ctttccgttg ggaatgaggc
     1501 gccctcggga gaggtcgaaa tagtccatcg ctttggtgat gtaaagatag gaattggcgt
     1561 caaaacgccg cacaaaatgg tccccccggt tgcgtaagta tccctccacc tcgaaatcgg
     1621 ccgcaaaatc aaaattatac tggccgtttt tcagctttcg ggaaaacttc tcttccatgg
     1681 attgatcgct catgaaggtg atatgcccga tcatgcgggc tacggctaat cccttctccg
     1741 gctgcccgtg ctcgtagtac tcgccctggc gccaggcggg atcggccatg atcgactggc
     1801 ggaccacttc atcaaaggca atctgctggg gagagtgctt caaggccgcg gcaatgggga
     1861 tggctgagcg gaccctctcc ccgtaagagg ccacccattg cagcgcctgc atgcccccca
     1921 tggacccccc ggttacgcaa agaagctttt caatcccgag gtgatctacc agatgacgtt
     1981 gggcttccac catatccgcg atcgtgatga ccggaaaatc gaggccgtag ggtttccccg
     2041 tcaggggatt gatggaagaa ggcccggtgc tgcctttgca tccgccgatc acgttcgagc
     2101 agatgacgaa gtagcggtcc gtgtcgaagg cctttcccgg accgatcatg acgtcccacc
     2161 agccgggacc tgattccccc ttgtgaaagc ctgcggcgtg cgcatcgccc gaaagggcat
     2221 gagccaccag gatggcattc gtcttgtcgc ggttcagtgt cccgtaagtt tcataggcca
     2281 gggttactgg gcttaacacc ctcccggatt ccagaaccag cgggcccgaa ggttccgcaa
     2341 aggtgaaata ttttgtcttc acaactccta cgcttccgtt tttattcatt ggataactga
     2401 atcccttctg cccgaatatc ctacgtgccc cctgttccct cgggttggga gaacaggggg
     2461 cgaggaacca ttgaaaagca attacttccc tgtcttcttc agagcctgct cgatatcttt
     2521 taaaatgtca tcgatatgct ccaggccgac ggagagccgg atgaagtcag gggtcacccc
     2581 ggtggccaat tgctcctccg gggacaattg ctggtgcgtc gtggtcgccg ggtggatcgc
     2641 cagggtcttg gcatccccca cgttggccag atgagagaca agttcgagag aattgataaa
     2701 tttcctccct gcctctattc ctcccttgat cccaaacccg agaatggcgc cggctccctt
     2761 cggaaggtat tttttgactc gccccttttc cgggctggaa gcgaggccgg gataattaac
     2821 ccaggccacc ctggcatgtt tttccaaata ttgcgcgacc gccagggcat tctccgaatg
     2881 ccggggaagc cgcaaatgaa tcgtctccag tccctggaga aagaggaagg cattgaaggg
     2941 cgacatggcc ggtccgaggt cccgcaggag tgttacccgg gccttgataa tataagccag
     3001 attgccgagg ggctttaagg cctcgacgaa tttgagccca tgatagctgg ggtctgcatc
     3061 ggcaatcaag gggaacttgc cattggtcca gtcaaatttg cccgaatcca cgatcaggcc
     3121 tccgattgag gttccgtgtc ccccgatgaa tttcgtggcc gaataaacga tgatatccac
     3181 cccgaaatcg atgggccgca tcaagtaagg cgagacggta ttatccagaa cgagagggat
     3241 gccatgctgg tgggcgaccc tggaaagacc ttccaggtcg gccacatcga gtttaggatt
     3301 cccgatcgat tcggcgtaga ccgctttcgt tttgggggta atggctttgt ggatcgcttc
     3361 cagatcattg gacggaacga acttcacctc aatgcccagc cgcggaaagg tatagtgaaa
     3421 gaggttgtat gttcccccgt agagattatc cgtggaaacg atttcatcac cggctcgggc
     3481 aatattgaga agggccagag tttccgccgc ctgcccgctc gccacggcca gggcccccac
     3541 cccgccgtct aataaggcga ctcgcttctc caggatgtca ttggtgggat tcatgatccg
     3601 ggtatagata ttcccgaatt ccttcagggc aaagagattg gcggcgtggt cggtatcctt
     3661 aaattggtag gaagttgtct ggtaaatcgg gacagctctc gaaccggtgg tcgggtcagc
     3721 ttcctgcccg ccatggaggg ccagggtttc aattcttaaa ttagcatcgt tcttgctcat
     3781 caatcctcct tggatttggt tcaggatttg cgacgatgtc tcatcctgac gttcatgaat
     3841 ttctttcttt gcatgcccat gtctccatcg cccatgccgc cttccgaatc catagccctc
     3901 acccccctcc accataaaaa aaaccccttc ctggttgata agaaggggct cctcttgctc
     3961 ttctcctcat cttatcttcc aaggtatcgc taccttgctg gaattagcac cagcatcccg
     4021 tctgagcggg accggttgct gtggcttctt cgggccagtc cctccgccac tctggataag
     4081 caggttttat tacctaacta atccttaatt tttggcactt tgtccatttt tatccgatta
     4141 aagccctccc gcgccccact ttcttttttc cttctttccc ttcccggcgg accctggacc
     4201 gcccttcttc taccagattt cccggcctca ccgtttggat aaagacttca caggttcggc
     4261 ataatttcat cttttgcggc caccccccct ggatttcccc ccggcagatc gtcccgttga
     4321 cgaaccagca gaggtggccc aggttaaatt cccacgccgg acacgcctct cggttcttcg
     4381 tagaacagtc ccgaatatcc cagcaggggc gggattttct gagtccctcc ccccaattgg
     4441 agattagaaa aagcacctgt cgttcaatgt gcaccgggac cttgcgccat ccctgctcga
     4501 agctctgcac ggctttgagg gaggttccga ggatttgggc catctgtttt tgagttttct
     4561 ggagtctttg gcgcagggaa ctgaactctc tggaatccaa ggtctctcct tcttttgaga
     4621 tccatcaaaa aggatattga cctatatacc acattgtggt attttaagtc aagaattatt
     4681 ttttgcaggc aattcaatgg aggggaaaga gaatatgggc gggatatcgg ccgcaatcga
     4741 aagggatatt cccaagaatc gagaggggcg ttttccccgg tttgaccggg aattaaacct
     4801 gaaagggaaa atctgtcctt atacctttat cgaatctatg ctcaccctgg aggagatgaa
     4861 gcgagatgag gtactgcggg tcatcgtgga ctatccgccg gcggtttgcg acgtcccccg
     4921 gagcttaaag aatgagggct atgaggtcct cgatgtgagc ccaatcaatg aaaccgattg
     4981 ggagatatgg gtacggaata aagaagtgga ataggcgcag atgaaattct ccgaggaaca
     5041 aattcaccga tattcccggc agatgatcct cccggggatc ggggggaaag gccagagaaa
     5101 actgggggag gcccgggtat tcatcatggg cgcgggaggg ctggggtccc ccgcagccat
     5161 atacctggcg gcggcaggtg taggggccat cggactggcg gactttgacc gggtggaact
     5221 gcataacctg cagcgccaga tcctccaccg gaccccggat ataggtctcc tgaaggtcga
     5281 ctccggcaag aagacgctgg aaaccctgaa tccggaagtc caggttcaag tttattctga
     5341 aagaatcacc tcggccaata tccgggacat cattaaagac tatcacctcg tcctcgacgg
     5401 gagcgacaat ttccccaccc gctttttggt caatgacgcc tgtttcttcg agaagaaaac
     5461 tttgatctcc ggggccatcc tgaggttcga cggtcagctc agcaccttca aacctcacgc
     5521 cggagggccc tgctaccgct gccttttccc cgaaccccct ccccctggca ccataccgag
     5581 ctgccaggaa gccggcatct taggggcggt cgccgggacc ataggcctcc tgcaggcgaa
     5641 tgaggcgcta aaggaaattt tgggcctggg gagatccatg agcggaaggc tccttctgtt
     5701 caatgcctta agtttatgct tcgatgaagt gaaaatcgaa cccaaccctg tctgccgcct
     5761 ttgcggaaag aaccctgcca taaaagagct gattgattac cccctcacct gccaggcctg
     5821 attctgaagc tgaatccctt ggggattcga tcccggatgg ctctttttcg gaggaatgac
     5881 ccccactgtc ctttctctct ctgtctctgg ttccagggcc taagaaacaa ggcaattcac
     5941 gatggataaa gttgcgtcgg gatcatagag attgaaagga aggggaaatg gtgatattct
     6001 ttcaatcaat tccataggat tcaagggagg gattccaatg ctgcggcgga gaattaaata
     6061 tttcctcttc cttgcctttt tctcctgttt gaccggtttc tgcggtcagg gacaggttca
     6121 agcattgacg gacgacgaga ggaataacgt aaccatttac cagaaagcga gtcccagtgt
     6181 ggtcaacgtg atcagcacgg tgatcacccg ggattttttc ttaaacgcca ttccccggga
     6241 gggaagcggc tccgggtcca tcgtcgacgc ccgcggccat gtcctgacca acaaccatgt
     6301 aatccgggac gcgaagaaac tggaggtgac cctggctgac agcagcaaat ggccggccaa
     6361 gctcgtggga accgacccgg acaacgacct ggcggtgatt aaaatcgatg ccccggcgga
     6421 caaactaaaa cccctccccc tgggggattc ccaaaagctc caggtcgggc agaaggtcct
     6481 ggccatcggc aatcccttcg gcttgggact cactctgact actggcatca tcagctctct
     6541 gggtcggaac atccggtcgg aggtggggac catgatcgaa gacctcatcc agaccgacgc
     6601 ctccatcaat ccggggaatt ccggcgggcc tctcctgaat tcggaaggcg agatcatcgg
     6661 catcaacacc gccattatca gtcccacggg ggccagcgtg ggaatcggct ttgccatccc
     6721 ggttaacact gccaagcgga tcattcccca gttgatcgcc aaggggtatg tgatctaccc
     6781 gtgggccggc gcctcggtgc agcctctttt cccggaactg gcccaggttt taaagctgaa
     6841 ggtggaaaga ggggccatgg tcatcgaggt cacccaagga ggaccggcgg aaaaggcagg
     6901 gctgcgcggg ggaaaccgcc agattcaagt ggggaacgtg gtcatcatcg tgggagggga
     6961 cgtgatcacc cggatcgatc agtcggaagt gaaagatgcc gacgatctaa ttaagcacat
     7021 tcgcgaacgc aaaccgggag aggtggtcat tctgaaggtt ctccgggacg ggcaattcaa
     7081 agaggtcaaa atcaccctgc aggaaaggcc gagagcgaca ggacgataaa aaacgttgct
     7141 ggttcctggg gctcgggtcc tttcatccgt ggtaagtgtt tcgctcccct ttggctgatt
     7201 tggaatccgg tatccatcat ccttcttcaa ccgggaaacc gcagcgaatt tcacttcatg
     7261 gaaaaccctc cgttgatgga aatcgtctgt ccggtgatcc aggagcagcg gtcggagcag
     7321 agaaagacga tcatgctggc cacatctccg gtttcaccca gtctcttgac ggtatacatc
     7381 ttcagccatt tctgttcgat ctccggggtc aaataagcgg tcatgggcga gtggacgaag
     7441 gccggcgcca cggcgttgac cgttatttta tcacgcccca gattcttggc cagggacttg
     7501 gtcagggaaa tcacccctcc cttggaagcg gcatagacct ccagcccttt ttccccggac
     7561 accccggcct cagaggcgat gttaatgatc cttccgatct tcttttcccg aagaatcggc
     7621 atcacggccc tgcagcagag gatggttccg atcaggttga catccaggat tctttgccac
     7681 tcgccccggg gggctttttc cagggggttc attcccccgc cgattcctgc gctgttgacc
     7741 agaaagtcga tcgtcccgaa atgggagagg gcttccctca ccatcctgga aacttgttct
     7801 tcctgggtca catcgcagga aatccctttc gcttctccct tccctctttt taatgaaccc
     7861 accgtgttct cgattcgctc ggcattcaca tccgccacca ggaccttgac cccttcttgt
     7921 acgaaaaact cggctgtctt ctcgccgatt cccgaagcac cgccggttac caccgccact
     7981 tttcccttta gatgcaagtc cattttcccc ccctttttcc tttccttggg tatcgtccgg
     8041 aataagaaag cccgtcagga tgatctcgct agaaatagaa tccccccctg acacattcgc
     8101 atgacgccag cgaataaaga gggggagagg aaattttctt gacttttcac ccgatcatcc
     8161 ttcgagattt tcctatcaaa ccccttcatg gatgtcaatg gccattgaaa aaatatttta
     8221 ctttgaataa agtaaagcaa agacgtcctt cagaaattgg aatcatattt agcctcagaa
     8281 ggccaagatc gccctgtatg gaatttcggt cgaagattcc ccgaacccta ccgatccggc
     8341 agaagggctt caccttaaca gaggtactct tcgtgctggc tattctgggc gttctcctgg
     8401 ccgtcgccgg cctcttctac tcggggtata gaaccaaagt gagcgaaacc gtggcccagg
     8461 aagacctgcg gcaagcctat aattcggcca tggccttttt tattgatttc ccgcaaagag
     8521 cccttactcg ggtcgacctg gagaaatacg gattccgatc ttcagcccga attgaaacca
     8581 gggtcgtcga cggtaggttg gaccacttgc tcctggtttc ctactacacg ctgcccggtt
     8641 caaaaatctt catgattgaa gggctgagag gagattttac cggaggcaag gaaccctctg
     8701 ggcctatcat tggacccgga ggctcgggtg gaggaggttc cttttccgat cctttgattc
     8761 agccgatgaa tgaaatgggt tggggaagat gtaatgataa agccaagacg gagctgaagg
     8821 aggctttcaa tgccgctcaa aaatatttcg caaaagaccc gggaggttcg atcaccaaag
     8881 acatcctacc ggattacggg taccttccca gcgaagaggt gaatctcttg gtcatcaacg
     8941 gcaaaaagcc cagcttggaa atttccgcca tattcagcgt tccaggagcg tcgaatttca
     9001 tgatcgattc ttcaggattc atcagggaag gttgagcaaa tccttctccg ctttgaacct
     9061 tccgaacctg ccccagcggg ggtgggcatt ccttcgagcg gatgtatttc ccccgttgtt
     9121 caagccctga aaggcagcct ttcaggggtg ttgacttcgg gctcttcctt tcataaactc
     9181 ctgggatagc tcagccggaa gtgactcccc caaaaggagg gaccatgggg aatcccaaag
     9241 actttattgg gacttcgaaa tggaatttgg atactccgtg tctggtcatc gaccaggata
     9301 tcctcgaaca aaacatcggg aacatgcagg ctcaccttac atccttcggc aagaagcttc
     9361 gtccccatgc caagacccac aaatgctcaa tcattgccaa gaaacaggtt gccgccgggg
     9421 gtattggaat ctgcgtggcg aaggtctccg aggctgaggt catggtgaag gctggcatgc
     9481 ggggggtcct gatcacctcc cccgtagtca ccgaccagaa gatcgtccgt ctcatggaat
     9541 gcctctttcg cgacccggac atcctggtgg ttgtcgacaa tcccaccaac gcccgcatgt
     9601 tgaatgaagc ggcccgaaag caaggcggcc gtctctcggt gctggtggat ttggacccgg
     9661 gcatggggcg taccggcgtc tctttccgcg agggcctcgc cctcggccag tggatccaca
     9721 gtctcccggc catgcgtctg aggggaattc aatgctatgc tgggaatgtt caacatatcc
     9781 tcggcttcca ggagcgaaag gaagcgtccc tcggttggtt gggtcaggct gccgaggttt
     9841 ttcgccaatt ccagcaaaac cggcttccca ctgaggtttt caccgggagt gggaccggga
     9901 cctacgaaat agactgccag gtccgtgagc ttaccgacct gcaggccggt tcctatatcc
     9961 ttatggatac cgagtatctt caaatcgggt gttcccagaa ccccaataaa tttgaaaaat
    10021 tcccccctgc cctcaccctc ctgaccacgg tgatcagctc caaccatccc ggctttttca
    10081 ctgtggacgc ggggttgaag gccctctatc accatggggg gactccatcg gtccttcatc
    10141 cggaaggtct cggcttggtt tatgaatggc ggggagatga acacgggaag atttccctcc
    10201 cggcccccgg aagaaaattc gaaatcgggg aggttctgga gctcgtcgtt tcccactgcg
    10261 atccaacggt aaacctcttt gactttttct acgtcacccg agctggaacc gtggtggatg
    10321 tatggcctat cgatctgcgc ggcaaaggcc agtagggaaa cgaggagaca gagggcagtc
    10381 gggcgattat tttacttcct tgttcttttc ttccttccac cacgaaggca aggttccatt
    10441 attctttttc ttcaattccg tttccaaagt gatgacctgt cctccaaacc gattgaattc
    10501 ttcccaggct tttttcttcc cttccacatc cttgcgggaa gaggcctcca gatatctctg
    10561 ataagcctgt tcgaattgct cgatgtagta agttttcttc tttcggtaat cctccatgcc
    10621 gaccccttgg agggtttccg cggaagagag cctttccgcc ggcaaaacct tttccacgat
    10681 attcatcgtt gccagggggg cccccggagg agttactggg ttttcgggag cgcgctgttt
    10741 ctctctggtg gaagtctggt ccgcgaattt attttctacc tcagtgatgg tgaacagatc
    10801 tttcttggac aaggaaacga ctccccccag ccactggaat ttgatctcac ccctctcctc
    10861 ccagaatcgg ggggtctcca acgtccggcc attttttagc ttgatgaggt agtcggccaa
    10921 ggccgggggg gtggaggtga gagtcaggag aatcgccaat ccaacgaata gctccctcat
    10981 tttttaccct tcgttgcctc gatttcaatc ttggagatca ggaaaagaac tctcctcatc
    11041 gcagggtcac tagggggagg taaatggtct gatcttgaac cgcccggctg caaaaaatga
    11101 tgttcgcacc tccccccatc aggtcggcgg agaccattcc cccggccatg attgcgccgg
    11161 tcacgttttt cccactgccg ccactaaaga taatggaccc cgttaccagg atgaccccgt
    11221 accactgaaa tgcgccataa acgcagaggt ccccttccac caaaagggtc ccgcacccat
    11281 gggaacctcc ggacagcctc acgtaggtgc cgttggtatt gaaatagacg atgttgcgga
    11341 ggctgcagtc ggaggggtca cgctgggtgg cgcctggaat gggggacccc cagtccattc
    11401 ccggcagagt ggcgctgttc acgtg
//
LOCUS       DASPRR010000585         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2239376, whole genome shotgun sequence.
ACCESSION   DASPRR010000585 DASPRR010000000
VERSION     DASPRR010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239376"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VEL68_21480"
     CDS             complement(<1..300)
                     /locus_tag="VEL68_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ38616.1"
                     /translation="MTGILNDLILGLSVTMSPNVLVYAVLGCVVGTLVGVLPGLGPLA
                     GISLLLPLTFGLSPTTAIVLLAGIYYGAMYGGSTTSILMRIPGETASVVTCLDGYE"
     gene            complement(303..791)
                     /locus_tag="VEL68_21485"
     CDS             complement(303..791)
                     /locus_tag="VEL68_21485"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ38617.1"
                     /translation="MMSMPPIYARLVAIVIAAVALIFIYGSLKMPMGTLLTPAAGLVP
                     LLLGCGTLVLAIVDIVSPEPAKAEGAISPEAEPQDQPDESRKVPRPLLIMIVFGLIIF
                     AFERAGFILSLLVGTFILLRFVESRPLVQSFIISLVLSSGLYFLFAKLLYVNLPDGWL
                     GF"
     gene            complement(788..1765)
                     /locus_tag="VEL68_21490"
     CDS             complement(788..1765)
                     /locus_tag="VEL68_21490"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ38618.1"
                     /translation="MRSLLKSLLLGLAILGLGTAPAPAQDFPTKPVTFIVPFPPGGLV
                     DFTARPVAAGLERVWKQPMVVSNRPGASGGVGMIAGANARPDGYTLLFTHASISTIPA
                     ADAIFGRPPSFDKSQFMPLALIVADPLIVIVRADSPWKTFEEFVAAARQAPGTIAYSS
                     SGAYGAIHVPVEMLAHAAGIKLKHVAYTGGGPMMTALLGGHVQVTVFSPAVVAPQIKS
                     GEIRPLLSTGAKRIALMPEVRTVYDLGYRDAEFYLWCGLFGPAKLPIPIADKIRAGVA
                     QAVRDPEFLKHMQNAGLPVEYMDAPDFRQFWMTDAERIETAIKRIGKVE"
     gene            complement(1989..3131)
                     /locus_tag="VEL68_21495"
     CDS             complement(1989..3131)
                     /locus_tag="VEL68_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXZ38619.1"
                     /translation="MMRDVAIIGVGMTNIGKFPDRMLSSMGREAVIAALKDAGVPPKE
                     IQAAYSGTSLGGALLGQRIFKDLGMSGMPINNMENACSSGSSAMREAWLSIASGLYDM
                     VIAVGSEKLSALGGGTLPLEREDLEVSGGMVMPALYAMRARRYMHEFGATPEHLAKVV
                     VKSRRCATKNPQAQFRKETTVEEVLNSRMVADPLTLFQCCPMGDGSAAAVLCSADKAR
                     QYTRQPILIKGSILVSGRFDNSFRDMTTPEITVRASKLAYEMAGIGPKEVSLAEVHDA
                     FSIAELLYYEALGFCGRGEAPKLLDEGATEIGGRIPVNPSGGLISKGHPTGATGLAQT
                     TEIVWQLRGQAGARQVKNAKVGLTHCTGGGIYGLDHGACSIHIFTK"
     gene            complement(3161..3586)
                     /locus_tag="VEL68_21500"
     CDS             complement(3161..3586)
                     /locus_tag="VEL68_21500"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXZ38620.1"
                     /translation="MEAPQEKKPVFFPDEGYWTTEADGLHLIGSRCNQCRRDYFPPRE
                     VCPKCFPEGLEHKMEKSKLASRGKLYTYSIVQVAPKRFLPPYALGYVDFPEGVRVLGQ
                     LTTTDPSQVKIGMTVQAELGRIAVDEQGNEVMSYKFRPM"
     gene            complement(3646..4836)
                     /locus_tag="VEL68_21505"
     CDS             complement(3646..4836)
                     /locus_tag="VEL68_21505"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXZ38621.1"
                     /translation="MAEILKGVRILDLSQYIAGPYGTLVLGDLGAEIIKIENPDGGDL
                     SRNIRQYTHKGESAYYLSLNRNKKSVTLNFRNPKAAEIFYDLVKVSDVVYDNFRSGML
                     EKFKIDYPTLKKINPRIISCSVTGFGSDSPYKDRPALDLLIQAYSGVMSFTGEPGRPP
                     VRMGIPMGDLSGAMFAVMAVLGALYYRQVTGQGQRLDISMVDGLLSLMTYRAQYYFLE
                     KEVPGPIGSGHVSQVPIRAYEAKDGKYLAIDSAQDKFFQNLCRVMGIEEVAKDARVNS
                     MGARLKNREVVDEILEKKFREKDRDEWVELLIQGDVPVGPVNNLAQALNNPSILARKM
                     VVPVKHLGEEVQMLGNPIKMSAIGEEVFEGAPTLGQHTGQVLQQVLGYSQEQIEFLRK
                     EKMV"
     gene            complement(4964..>5298)
                     /locus_tag="VEL68_21510"
     CDS             complement(4964..>5298)
                     /locus_tag="VEL68_21510"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HXZ38622.1"
                     /translation="DLRSYFSLNLKFHRLLSRVGGNQRLHQILENFGKQTHRFRYASM
                     SLPGRMPKSSSYHQRLMEAIQRRDEEKAEKIARTIIEEAGRALIEHPFDESSDFPELF
                     KKTLPGKA"
BASE COUNT         1066 a         1602 c         1539 g         1091 t
ORIGIN      
        1 ctcgtatccg tccagacagg tcacgaccga agcggtttct cccgggatcc tcatcaggat
       61 ggaggtcgta gacccgccat acatggcgcc gtagtagatt cctgccaaca gcacgatggc
      121 cgtcgtcggg gacagaccga aggtcaaagg cagaagcaga ctaatcccgg ccaaaggccc
      181 aagtcccggc agaactccga ccagcgttcc cacgacgcat cccagcacgg cgtagacgag
      241 aacattgggg gacatggtga ccgacaggcc gaggatcaga tcattcagga taccggtcat
      301 gatcagaacc ccagccatcc gtctggaagg ttcacgtaga gaagcttggc gaagagaaaa
      361 tacaggccgc tcgacaagac cagcgagata atgaacgact ggacgagcgg cctgctttcg
      421 acgaagcgga gcaagatgaa cgtgccgacc agcaggctca gtatgaatcc cgcgcgctca
      481 aaggcaaaga tgatcagccc gaacacgatc ataatcaaga gcggtcgcgg cactttcctg
      541 gattcatccg gctgatcctg gggctcggcc tccgggctga tggcaccttc tgccttcgcc
      601 ggctctgggc tgacgatgtc gacaatggcc agcaccaggg ttccgcaccc gagcagcagc
      661 ggaaccaggc cggccgccgg ggtcaggagg gttcccatcg gcattttcaa cgagccgtag
      721 ataaaaatga gcgcgacagc agcgatgaca atggccacca gcctcgcata aatcggaggc
      781 atcgacatca ttcaaccttc ccgatgcgct tgattgcggt ctcgatgcgc tcggcgtccg
      841 tcatccagaa ttggcggaaa tccggcgcgt ccatgtactc caccggcaag ccggcgttct
      901 gcatgtgctt caggaactcg ggatcgcgaa ccgcctgggc tacacccgcc cgaattttat
      961 cggcgatcgg gatgggcagt ttcgccggtc cgaaaaggcc gcaccagaga tagaattcgg
     1021 cgtccctgta gccgagatca tacaccgtcc gcacctccgg cataagggca atgcgcttcg
     1081 cgcccgtgct caaaagcggt cgaatttcgc cgctcttgat ttgcggggcc accacggctg
     1141 gcgaaaacac ggtcacctgg acgtgcccgc ccaggagtgc cgtcatcatt ggcccgccgc
     1201 ctgtataggc gacgtgttta agcttgatgc cggcggcgtg ggcgagcatc tcgaccggga
     1261 cgtgaatcgc gccatacgct ccggatgacg aataagcaat cgtcccgggc gcctgacgtg
     1321 ccgcggcgac gaattcttca aacgtcttcc agggcgaatc ggcccgcacg ataacgatca
     1381 gcggatcggc cacgatcagc gccagcggca tgaactggga cttgtcgaag ctggggggcc
     1441 ggccgaagat cgcgtccgct gcggggatcg tcgagatgct ggcgtgggtg aatagaagcg
     1501 tgtaaccgtc cggccgggcg ttcgccccgg cgatcatccc gacgccccca ctggctcccg
     1561 ggcgattgga aaccaccatg ggctgcttcc acacccgttc gagccccgcg gcaaccggac
     1621 gcgccgtgaa atcaaccagg ccgcctggcg gaaacggaac aatgaaggta accggttttg
     1681 ttggaaaatc ctgcgcaggg gcaggggcag tccccaggcc cagaatcgcc agcccgagaa
     1741 gcagcgattt cagaaggctc cgcattttcg ttcctcctcc tccattctgg acgacaggtt
     1801 tttccccgcg tttgggtaca ggcggcgtcg tcttttccgg ccgttgaaga tcagaccacc
     1861 cgtttatacc accagcggca gagtgaaagc aatcccgcgc gttgcgggat tggcaagaac
     1921 aagaaatgct attcccccgg ccagcgtggg ggcaattcat gaatcgcccc tacaggagcc
     1981 tccccggatt acttcgtaaa gatgtggatg gaacaggccc cgtgatccag gccgtagatg
     2041 ccgcctcctg tgcagtgggt gaggcctacc ttggcgttct tcacctgccg cgcgccggct
     2101 tgcccgcgga gctgccagac gatctccgtt gtctgggcca gtccggtggc tccggtggga
     2161 tggcctttgg agatgaggcc tccgctggga ttcaccggaa tccgtccgcc gatctccgtt
     2221 gccccctcgt cgagcaactt gggagcctcc ccccggccgc agaagcccaa ggcctcgtaa
     2281 tagagcagtt ccgcgatgct gaaggcgtca tggacttccg ccaggctaac ctccttaggc
     2341 ccgatcccag ccatttcgta agccagcttg gaggcccgga cggtgatctc gggagtggtc
     2401 atgtcccgga agctgttgtc gaacctgccg gagaccagga tggaaccttt gatcagaatg
     2461 ggctggcgcg tgtattgacg cgctttgtcc gcgctgcaga gcactgcggc ggcggaccca
     2521 tcccccatgg ggcagcactg gaaaagagta agcgggtcgg ccaccatccg ggaattcagc
     2581 acctcctcta ccgtggtctc cttgcggaat tgggcctggg gattcttggt agcgcaccgc
     2641 cggctcttca ccaccacctt ggccaggtgc tccggggtgg ctccgaactc gtgcatgtac
     2701 cggcgggcgc gcatggcata gagggccggc atcaccattc ccccgctgac ctccaagtcc
     2761 tccctttcca gggggagggt tccgccgccc agggcggaaa gcttttccga tcccacggca
     2821 atgaccatgt cgtacagccc gctggcaatg gacagccagg cctcccgcat ggcgctcgaa
     2881 ccgctggaac aggcattctc catgttgtta atgggcatcc cgctcatgcc caggtccttg
     2941 aagatgcgct ggccgaggag cgcacccccg agcgaggtgc ccgaataggc ggcctgaatc
     3001 tccttggggg gaacccccgc atccttgaga gcggctatga ccgcctcccg ccccatgctg
     3061 gaaagcattc gatcgggaaa ttttccgatg ttagtcatcc ccacgccaat gatagctacg
     3121 tccctcatca taggtctcct ttcctctttg cgaggtgcgg tcacatgggc ctgaatttat
     3181 agctcatcac ttcattgccc tgctcatcga cggcaatccg gccgagctct gcctgaaccg
     3241 tcataccgat cttgacctgg gaggggtcgg tggtagtcag ctgcccgagc actctgaccc
     3301 cttcaggaaa gtccacatac cccagggcgt aggggggaag gaatctcttg ggggccacct
     3361 gcacaatgga ataggtatat agcttccccc gactggcaag cttggacttc tccatcttgt
     3421 gctcgagtcc ttcgggaaag catttggggc aaacctccct tggaggaaaa tagtctcgcc
     3481 ggcattgatt gcagcggctg ccgatcagat gcaggccgtc tgcttccgta gtccagtacc
     3541 cttcgtcggg gaagaatacg ggcttttttt cttggggtgc ttccatgaga aatatacctc
     3601 ctctcttcgc ttttgcatct ttgagcaggg atgatcggtg gtctcttaga ccatcttctc
     3661 tttccgcaaa aattcgatct gctcctggga atatcccagg acctgctgaa ggacttgacc
     3721 agtatgctgg cccagggttg gggccccttc aaagacctct tcgccgatcg cggacatctt
     3781 gattgggttt cccagcatct ggacctcttc tcccaagtgc ttgaccggga ccaccatctt
     3841 gcgggccaat atggatgggt tgttcagggc ctgggcgagg ttgttgaccg gtccgaccgg
     3901 cacatccccc tggatcagca gttccaccca ttcgtcccgg tccttctccc ggaacttttt
     3961 ctccaggatc tcatctacca cctcccggtt cttcagcctg gcgcccatag aatttacccg
     4021 ggcgtccttg gccacttcct cgatccccat gacccggcag aggttctgga agaatttgtc
     4081 ctgggccgaa tcgatagcca ggtattttcc gtctttggcc tcataggccc ggatgggaac
     4141 ctgggagaca tggccggacc cgatgggccc gggaacctcc ttctccagga aatagtactg
     4201 ggcccggtag gtcatgagcg aaagaagtcc gtcgaccatg gagatgtcga gcctctgccc
     4261 ttgacccgtc acctgacggt agtaaagggc gcccaggacg gccatgacgg cgaacatagc
     4321 cccactcagg tctcccatgg gaattcccat gcggaccggg ggccgccccg gctccccagt
     4381 gaagctcatg accccggaat aagcctggat caggaggtcc agggcgggcc ggtctttgta
     4441 cgggctgtcg gagccgaacc cggtaaccga acaggagatg atccgggggt tgatcttctt
     4501 cagggttggg taatcgattt tgaatttttc cagcatgcct gaccgaaagt tgtcgtaaac
     4561 cacgtcggaa accttcacca agtcgtagaa gatttcggcg gcctttgggt tgcggaaatt
     4621 cagagtgacg gacttcttgt tccggttcag gctcagatag taagcgctct cccctttatg
     4681 ggtatactgg cggatgttcc ggctgaggtc tccgccatct gggttttcga ttttgataat
     4741 ctcggcgccc aggtctccga ggacgagggt cccgtaaggc ccggcaatgt actgggaaag
     4801 gtccaggata cggacccctt tcagtatttc agccatagcg acctccttgg aaaggaatgt
     4861 ttaggtagaa ccgcatgaat tcgatccctt tgggtaacat gagaagaaag cccgtctttc
     4921 aggcaggaga cggcgaaatt ccctgaagcg tccattttcc ggctcaggcc tttccgggga
     4981 gcgttttttt aaaaagctcg gggaagtccg atgactcatc gaaagggtgc tcgatcagcg
     5041 cccgaccggc ctcctcgata atcgtccggg caattttctc ggctttttct tcatctcgcc
     5101 tctgaatggc ctccatgagc cgctggtgat aggagctcga tttcggcatc cttcccggga
     5161 ggctcatgga ggcataccgg aaacggtgcg tctgcttccc gaaattctcc aggatttggt
     5221 gcaatctttg attccccccc actcgggaaa ggaggcgatg aaatttcaag ttcaaagaaa
     5281 aataggacct caggtcgt
//
LOCUS       DASPRR010000586         6240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2080889, whole genome shotgun sequence.
ACCESSION   DASPRR010000586 DASPRR010000000
VERSION     DASPRR010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080889"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VEL68_21515"
     CDS             <1..516
                     /locus_tag="VEL68_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38623.1"
                     /translation="TNNLWTTPETATIRKVQAEEKAFHKAYAQKLGLQISPEEVKQMG
                     IETFTAMSGASKQEIEKGFAKVREEMAKIKGYPIRTVVNWSVEGDKAEGAEKEGKGSS
                     ESSSSTPTSVGGFLSGLSKTLGQKVAKDQSSGSGQKEAATFSTTHEVKAIRADSVPAE
                     VFDVPPGYSKK"
     gene            539..1033
                     /locus_tag="VEL68_21520"
     CDS             539..1033
                     /locus_tag="VEL68_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38624.1"
                     /translation="MKIWMLTLVILLSLGSPALAQELKRISLDDASNLGLQIQSDSTV
                     KVEGKASIKITTKWPTTVCLAEVPGPDIENAMLVYKAKVKSDLQGQGTAFLEMWAHVG
                     GGQYFSRGMNDVVQGKSDWKTIQTPFMFQKGQKPTKVTLNIVINGPGTVWVDNIVLSK
                     EPLK"
     gene            complement(1265..2110)
                     /locus_tag="VEL68_21525"
     CDS             complement(1265..2110)
                     /locus_tag="VEL68_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ38625.1"
                     /translation="MEVKEFQDIKYKKDEQTGIVLVTLNTPKRKNAMSPYTFLELWWA
                     VDAMEKDEGARAMILTGAKDPDSNDPAKEAFSSGGYFNLAAMVKLREQFKDQIDFTDI
                     AQKKLTLKMWQFDKPVIAAINGLVVGGAFTMCLSCADLIYASEHAWAQLPFVNIGIIP
                     ELGSSYLLPRLLGFQRAKELIYLGERIPAQQLFELGLVNKVVPHDQLIPYAKEMALRL
                     IPPAGAWMAIRAAKRALHKPMLQEITEALDRENQGLNQAFGTEDFREALKARAEKRPP
                     VFRGK"
     gene            complement(2294..2518)
                     /locus_tag="VEL68_21530"
     CDS             complement(2294..2518)
                     /locus_tag="VEL68_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38626.1"
                     /translation="MKGSPPKGGHHLDANAGPRGKGLGSKNHEASFISWAGSATRSRN
                     PVEALVLVGATSRSPDRAPAQAEASPSLQE"
     gene            complement(2568..3632)
                     /locus_tag="VEL68_21535"
     CDS             complement(2568..3632)
                     /locus_tag="VEL68_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004405869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXZ38627.1"
                     /translation="MGDTFPKFKAAAIQAAPVFLNREATVDKAGRLIEEAAAKGAELI
                     AFPEVYIPAYPWWHRLDNPYRGHKYFRELVKNSVEIPSPALDRICECARKFNVFVVMG
                     INERVPETLGTLYNTNVLIDRKGKILGAHRKLVPTFAEKLTWGGGDGSTLKVYDTDIG
                     KLGMLNCGENTNSLARYALIAQGEQVHIANYPGQPAGDESNYDLAHAIEIRSAGHAFE
                     GKLFNVVSCCVFTPEIAKVLGDTEEKKRMVSNGSMGLTAIYGPDGKRLAGPLDPNEEG
                     MVIAEIDIEQIIVQKLQHDIVGHYQRFDVLSLNLNRRPLKPIWETGAGEERGSLQRKE
                     MDELKSLVAGLAEKVQGGKS"
     gene            4049..5623
                     /gene="rmuC"
                     /locus_tag="VEL68_21540"
     CDS             4049..5623
                     /gene="rmuC"
                     /locus_tag="VEL68_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXZ38628.1"
                     /translation="MDNPILILSVIGAALVGALVCWLSLRAKISQVQAQGESEKAILS
                     ERLLGREQQVQELKDSVDRLSAEMDRQREELKFESQKRSAAEERNCRITELEADLRRK
                     EEQLDKLQGEATDLKTKISELGIKIEDERKAAQEKLAMLDEARQKLSDAFKALSAEAL
                     RSNNQSFLDLAKATLERFQEGAKGDLEMRQKAIDALVQPLKESLEKVDGKIQEIERNR
                     TTAYASLTEQIKMLASSQSMLQSETANLVKALRSPTVRGRWGEIQLKRVVEIAGMLEY
                     CDFIQQESVTTDDGRLRPDMVIKLPNNKNLVVDSKAPLQAYLEALEAPDDEIRMAKLK
                     DHARQIRNHLSKLSNKAYWDQFKPTPEFVILFLPGEIFFSAALEQDPSLIEAGVDQRV
                     ILATPTTLIALLRAVAYGWRQELVAANAQAISELGRILYERLRTLSGYFTELRRGLDR
                     AVDAYNKAVGSFEGRVLVSARRFKDLGASTGEEIETLEAMDKSTRPIVEDEGPAAAGI
                     PAKEGEARALPGGEEG"
     gene            complement(5626..6099)
                     /locus_tag="VEL68_21545"
     CDS             complement(5626..6099)
                     /locus_tag="VEL68_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXZ38629.1"
                     /translation="MASNGEKMKGRVGRDSFSGIIKTEAWVKRTKWIFLFLFVLLLLS
                     AIACQTTENRRAEIRCRPSAVSPRSAVESGAITIGWNPNQEPGLAGYRVYYGTSAGSY
                     NSCVDVGNPPKSSSGLIEYTLTALDKGKKYYIAVIAYDNYQNMSGFSIEVSDIAK"
BASE COUNT         1475 a         1629 c         1773 g         1363 t
ORIGIN      
        1 acgaataacc tctggactac cccggagacc gcaacgatcc gaaaggtcca ggcagaagag
       61 aaggcgtttc ataaggccta cgcccagaag ttgggactgc aaatatcacc ggaagaagtc
      121 aaacagatgg gaatcgagac ctttaccgcc atgtccgggg cctccaaaca ggagatcgaa
      181 aagggattcg cgaaggttcg ggaggagatg gccaagatca aagggtatcc catccggacc
      241 gtggtgaatt ggtcggtgga aggggataaa gccgagggtg cggaaaagga aggaaaaggg
      301 tcgtcggaat cttcttcctc aacccccacg agcgtgggcg ggttcttaag cgggctgagc
      361 aagacccttg gccaaaaagt ggcgaaggac caatcctccg gctcaggcca gaaggaagca
      421 gccaccttca gcactaccca cgaagtgaag gcgatccgtg cggattcagt cccggccgaa
      481 gtcttcgacg tccccccggg atattccaag aaatgaccga ccgaaggaga agatcgagat
      541 gaaaatatgg atgctaacct tggtgatatt gctgtcgctc gggagtccgg ccttggcgca
      601 ggaattgaaa aggatcagcc tggacgatgc ttcaaatctg ggactgcaga ttcaaagcga
      661 ttcgacggtg aaagtagaag ggaaagcctc gatcaaaatc accacgaaat ggcccacgac
      721 ggtctgcctg gcggaagtgc ctgggccgga cattgaaaac gcaatgttgg tttataaggc
      781 taaggtcaaa agcgaccttc agggtcaagg cacggccttt ctggagatgt gggctcacgt
      841 gggaggcggg caatattttt cccggggtat gaacgacgtg gtccagggta agtcggattg
      901 gaaaacgatt caaaccccct ttatgttcca gaaagggcag aagccaacca aggtcaccct
      961 gaacatcgtg atcaacggtc cggggacggt ctgggtggat aacatcgtat tatccaaaga
     1021 acccctgaaa tagccctttt cccttagtac taatagggcc aaaattatat gggtgctgaa
     1081 gcctaagccg ttcgtgctga gcttgtcgaa gcatgagtta gtcgaagcat gaacggcgta
     1141 cgatgcaccc ttcgacaagc tcagggtgaa cggtgacagc ccaatgaaga gcatcgggat
     1201 tgggagagat tttccgaaaa ctatgtcttc acaattatgt acttatcagt aatttaaccg
     1261 gtttctattt cccccgaaaa acgggcggcc ttttttccgc cctggccttt aacgcttccc
     1321 ggaagtcttc ggtcccgaaa gcctggttga ggccttggtt ttccctgtcc agcgcttcgg
     1381 tgatctcttg tagcatgggc ttgtggaggg cccttttggc cgcccgaatc gccatccagg
     1441 ctccggcggg cgggatcagc ctgagagcca tctcttttgc gtaggggatc aactggtcat
     1501 gagggacaac cttattgacc agccctaact cgaacaactg ttgggccggg atccgctccc
     1561 cgaggtatat caactctttg gctctttgga atcccaggag gcggggcaag agatagctgg
     1621 agccaagttc cgggatgatc ccgatgttca cgaagggaag ttgggcccag gcatgctctg
     1681 aggcataaat caggtcggca caggaaaggc acatggtaaa agccccaccg acgaccagcc
     1741 cgttaatcgc ggcaatgacc ggtttatcga actgccacat cttcagggtg agcttcttct
     1801 gggcgatgtc cgtgaaatcg atctggtctt tgaactgttc ccttaatttg accatggccg
     1861 ccaggttgaa atatccgccg ctgctgaagg cctctttggc agggtcgttg gaatcagggt
     1921 ctttggcccc ggtcaggatc atcgccctgg ctccctcatc cttttccatg gcgtccacgg
     1981 cccaccagag ttcaagaaag gtatacgggc tcatggcatt cttgcgcttg ggagtgttga
     2041 gggtcaccag gacgataccg gtctgctcgt cctttttata cttgatatct tgaaactcct
     2101 ttacctccat aggcgctccc ttcctgaaga cgaatcaaag atcattgatc actgtccatt
     2161 gccctggacc taattttatt ctggtaaacc cccggctctg cggggggctc aaagagtttg
     2221 acatttccgg gatgtcggag ttgtggcccg ggggcaaggg tctggggttc aaaaagcatc
     2281 ggccgggcga tccctattcc tggaggctgg gactggcttc cgcttgagcc ggcgcccgat
     2341 cgggcgaccg gctggtcgcc cctacaagga ccagagcttc cacggggttg cgggaacggg
     2401 tggccgaccc ggcccatgaa atgaaggagg cctcatggtt tttggacccc agacccttgc
     2461 ccctgggccc ggcattggcg tccaggtgat ggccaccctt aggcggcgac cctttcaatc
     2521 cgctttgagc ggttcactgt ttttcatgcc tccggctctg ccggaggtca tgatttgcct
     2581 ccctgcactt tctcagccag tcccgcgact aaggatttga gttcatccat ctctttcctt
     2641 tggagggagc ctctttcttc cccggcgcct gtctcccaaa tgggtttcag gggacggcgg
     2701 ttgaggttca gggaaaggac atcgaaccgc tggtagtgcc cgacgatgtc gtgctgcagc
     2761 ttctgcacga tgatctgttc gatgtcgatc tcggcgatga ccatcccttc ttcgttgggg
     2821 tccagggggc cggccaggcg cttgccgtcg gggccgtaaa tcgcagtcaa ccccatactt
     2881 ccattgctga ccatcctctt tttttcctcc gtatctccca ggaccttggc gatttccggg
     2941 gtaaagacgc agcaggagac cacgttgaag agctttcctt cgaaggcatg gcctgcgctg
     3001 cggatttcga tggcgtgggc caggtcataa ttggattcgt ccccggccgg ctggccgggg
     3061 tagttggcga tgtgaacctg ctctccctgg gcgatcaggg cgtaccgggc caggctgttg
     3121 gtgttctccc cgcagttaag catccccaat tttccgatgt ccgtgtcgta aaccttcagg
     3181 gtggagccgt ctccccctcc ccaggtcaat ttttcagcaa aggtcggaac cagcttgcga
     3241 tgggcgccca ggatcttccc ttttcggtcg atgaggacgt tggtattgta aagggttcca
     3301 agggtctccg gcaccctctc gttgatcccc atgactacga agacgttgaa tttccgggca
     3361 cactcacaaa tgcgatccaa tgccgggcta gggatttcca cggaattctt aaccagttcg
     3421 cggaaatact tgtggccccg gtagggattg tccaagcgat gccaccaggg gtaggccggg
     3481 atgtaaactt cggggaaagc gatgagctcg gcccccttgg cggcggcttc ttcgatcagg
     3541 cggccggctt tatccaccgt cgcctcccgg ttcagaaaaa cgggagcggc ctgaatggcc
     3601 gcggccttga atttcggaaa agtatctccc atcgatgacc tcctttttcg ttcaccaaag
     3661 agacacggag aacacagcca ggcggaacct agccaaatcg taggggcaac ccccctgtgg
     3721 ttgcccatct tgagggcggc cacggggggc cgcccctaca tttcaaaaaa cttaataaga
     3781 tttagagatc ctgcatgtta gtagcacaga ggattttaaa taggcattca tccgaattcc
     3841 agggttcaag gacaatttag agaaattgtt attcggtgtc aatttgtatt tttttcatga
     3901 tgaggcttct tttttgattc gcccggggag gggcgattca tgaatggccc cttctaccaa
     3961 tcgtaaaaaa ggaaggggat accattattg ggggataaat gataagattc ctgtggcttt
     4021 atagaatcgc agcgatggag ttgtagagat ggacaacccg atcctcatcc tctcggtcat
     4081 tggggcagcg cttgtcggag cccttgtctg ctggctgtcc ctccgggcta aaatcagcca
     4141 ggtccaggct cagggggaat ccgaaaaagc cattctgagc gagcgactcc tgggaaggga
     4201 gcaacaggtc caggagctta aagactcagt ggacaggctt tctgctgaaa tggaccgcca
     4261 gagggaggag ctgaaatttg agtcccagaa aaggtcggct gcggaagaga gaaactgccg
     4321 cataaccgag ctggaagcgg atttgaggcg gaaagaagag cagttggata agctccaggg
     4381 cgaagccacg gacctgaaaa ccaaaatttc cgaactgggc atcaaaatcg aagatgagcg
     4441 gaaagcggcg caggaaaagc tggcgatgct ggatgaggcc cggcaaaaac tttccgacgc
     4501 cttcaaggcc ctttcggcgg aagctcttag aagcaataat caatccttcc tcgacctggc
     4561 caaagcgacc ctggaaagat ttcaggaagg ggcgaaaggg gacctggaaa tgaggcagaa
     4621 agccattgat gccttggtcc agcccttgaa agaatcgctt gaaaaagtgg acggcaagat
     4681 tcaggagatc gagagaaatc gcaccaccgc ttatgccagc ctgacggaac agatcaagat
     4741 gctggcttcc agtcagtcca tgctgcagag cgagacggcc aacctggtaa aggccctccg
     4801 aagcccgaca gtccggggaa ggtgggggga gattcagctg aaaagagtgg tggagatcgc
     4861 cggaatgctg gaatactgcg atttcatcca gcaggagtcc gtgactacgg atgacggccg
     4921 gctgcgaccg gacatggtaa tcaaactgcc caataacaaa aacctggtgg tggactccaa
     4981 agctcccctg caggcctatc tggaggccct ggaagcccca gatgacgaga tcaggatggc
     5041 aaaactcaag gatcacgccc gccagatacg caatcatctg tcaaaattga gcaacaaggc
     5101 ctactgggat caattcaagc ccaccccgga gtttgttatc ttattcctgc ccggagaaat
     5161 cttcttcagc gccgccctgg aacaggaccc gagcctcatc gaggcggggg tggaccagcg
     5221 agtcatcctg gccacaccta caaccctgat cgcccttctg cgggctgtgg cctacggatg
     5281 gagacaagaa ctcgtggccg cgaatgccca agccatcagt gaattgggaa ggattcttta
     5341 tgaaaggctt cgcaccctgt ctggttattt tacagaactc cgcaggggcc tcgatcgtgc
     5401 ggtggatgcc tacaacaagg ccgtagggtc ttttgaagga agggtgctgg tgtcagcccg
     5461 aagattcaag gacctgggag cttcgaccgg ggaagagatt gaaactctgg aggccatgga
     5521 caaatccacg cggcccatcg ttgaggacga agggccagcg gcagcaggaa tcccggccaa
     5581 ggaaggggaa gccagagccc ttcccggggg ggaagaggga tgaggtcatt tggcgatatc
     5641 gctgacctca atcgagaatc cactcatgtt ttggtaatta tcgtaagcaa tcactgcaat
     5701 gtagtacttt ttccccttat ccagtgcggt taaagtatac tctatgaggc cggacgagga
     5761 cttgggtgga ttcccgacat caacgcagct gttatagcta cccgcagacg tcccatagta
     5821 gactcggtaa cctgccaggc cgggttcctg gttgggattc catccgatgg ttatcgcccc
     5881 gctttcaacc gcggacctgg gagagacagc gctgggtctg catcttattt cagcccttct
     5941 attttcagtg gtttgacagg caattgcact gagtagaaga agaacgaaga ggaataaaaa
     6001 aatccatttt gtcctcttaa cccatgcctc cgttttgata ataccggaaa aagaatctct
     6061 tccgaccctg cctttcattt tttctccatt cgatgccata acaagccccc tttgttaagg
     6121 tgatgaaaat gcagagaccc cattcttcaa agaagagaaa ttcggtcagt acttcatccc
     6181 cgtaaacgcc actccctcga tgatccgggt gcgaaaaatc agaaagatgg ccagcaccgg
//
LOCUS       DASPRR010000587         8171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_289072, whole genome shotgun sequence.
ACCESSION   DASPRR010000587 DASPRR010000000
VERSION     DASPRR010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289072"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VEL68_21550"
     CDS             <1..194
                     /locus_tag="VEL68_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase"
                     /protein_id="HXZ38630.1"
                     /translation="ARARTARLNEWLTLGAGVPGCMHGGGSPDGARLVVRSLLPLEKY
                     AQMARKLGGIKEEIPDPGK"
     gene            318..446
                     /locus_tag="VEL68_21555"
     CDS             318..446
                     /locus_tag="VEL68_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HXZ38631.1"
                     /translation="MPNKVGQVYVCEICGNKVEMVVAGKGTLHCCGQPMKLAGEKK"
     gene            553..2421
                     /locus_tag="VEL68_21560"
     CDS             553..2421
                     /locus_tag="VEL68_21560"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ38632.1"
                     /translation="MDGYAGKILRVNLTTRRCTEEPLNPKYAKDYIGGTGLGVRMIYD
                     EVPPCTDPLSPENKIVFTVGPVTATNYPSGARYQIVFKSPLTGILCDASSGGYWGADL
                     KRAGYDALMIEGAADDPVYLWIHNGKTEIRPATHLWGQDALKVQESIQREIGDERIRV
                     ACIGPAGERKVLLSCIINDEGRAPGRGGNGAILGSKNLKAIAVRGNGNFPIHDLATYN
                     TLCKKIAKDNAISPAVAAMREYGTAQVLDNLWAMGDIPVKNWQLGIWEEGCKNLGGKK
                     MKETILVPHTACYRCTLGCSRWVKIEEGPYKMDGPGPEYETLAAMGTMCLIDDLNAVS
                     FANDLCNRYGIDTISTGVAIAFGMEAYEKGLIKKEDTGGIELKWGDAQAMIAMTHQIG
                     KNEKLGALLGQGVKRAAEKLGGDSWKYAVHVKGMETAMHDPRTFYSIGLTYAVGPRGA
                     CHLHGHSPLWENVQDPLPEWGLKGMYPLHVSKGKGLVAKLSQDYTAVVNSMVSCYFLT
                     FILKPSDLAAVLSSATGTKYTAKSLLKVGERIMALHRAYNNLCGITRKDDVLAPRQLE
                     ATKEGGNAGKVPDMEVMLKEFYKVSGWTPSGKPSRKTLESLGLRDVAKDLYGPKGK"
     gene            2576..3610
                     /locus_tag="VEL68_21565"
     CDS             2576..3610
                     /locus_tag="VEL68_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HXZ38633.1"
                     /translation="MAEEKKFTYPAGKLPLEDLSRLLSRFARNDPRIVIPPGIGKDAT
                     VISFGDKYLVAKTDPITFATDQIGWYAVHINANDVAAMGGVPQWFLATLLLPAGKTGP
                     EEVEEIFSQVSTACEELRIILCGGHTEISYGIDRPIVVGQMLGEVEKEKLVSPDKIRS
                     GDEIILTKGIAIEGTALIARELREINSLLEEKRLDRCRNLLRSPGISVVREARIANEV
                     AAVHAMHDPTEGGLATGIQEMANAAGMGMRVEMDQIHILPETAILCEKLKLHPLGLLA
                     SGAMLIAVAPKDSTKVIQALEKEGISASVIAKVWEREKGVKLAERGKITDLPFFARDE
                     LARLFDGSKG"
     gene            3607..3882
                     /locus_tag="VEL68_21570"
     CDS             3607..3882
                     /locus_tag="VEL68_21570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38634.1"
                     /translation="MSSFTTRYRNKKFVKERVELDRHAYENCEFEGCMIVLEKGETEI
                     KGCKFNHCRLVLLGQALRIAKVLSLFTGGKPLKVLDFAEPGVFGGKK"
     gene            4006..6078
                     /locus_tag="VEL68_21575"
     CDS             4006..6078
                     /locus_tag="VEL68_21575"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ38635.1"
                     /translation="MIIKTYCARMDHGGCGLRVHVEDGKIVKVEGDQDSPLNRGTVCG
                     KGIAQVERINHPDRLRHPLKRIGERGEGKWECISWEEALTTLSQRFLQADRRSIAFAQ
                     GTPKGLELFMMIRLANLLKVATVVTPGSICHMPRETAAMITCGFFPVPDYDHPPALVI
                     VWGSNLFQTNEEGIIGSQLRRVLDQGAKLIVIDPRRTQLSTRADLWLQPRPGTDLTLA
                     LGMLKVIVDGGLHDRAFVDQWTVGFPELQEHLKDYSLKTLSETTWVPEEKITETARFY
                     ARMRPACIQWGNAIEHTIQSVQTARALMILKAITGNLDSPGGNVNRPSPPTLRPGDFV
                     QGKSFPEKREELLAPEFRLAAMMGFTPSQTVVKAILSGKPYPIRMMYIQGGNPLLSYA
                     NATETYLALKKLDFLAVAEIFLTPTAQLADIVLPTATNFEFDDMGHYGMPHGFLLARP
                     KVVDPPGECLPDLLILNDLGKRCGLGKFFWPDLRSCLDEVLKPAGVNYEAFKEAGMLK
                     GNWKYKSYERSGFATPSKKVEIFCSRLKDWGYDPLPTYRQEFSAPDSDPHRMENYPLI
                     LTSAKDPCYFHSAYRNLPSLRKFSPDPVVLLHPQTAEGMGVKEGDWVHIETQRGTIRQ
                     KAAFHAELDPRVVVAAYGWWFPERKDLELSGWKESNINILTENDPPYDPTIGSTNLRA
                     IPCRVYKE"
     gene            6211..7017
                     /locus_tag="VEL68_21580"
     CDS             6211..7017
                     /locus_tag="VEL68_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ38636.1"
                     /translation="MNPSSEGKSAYKNWIKFIGTAGARFVVARQLRASGGLWLSFQGA
                     NLYMDPGPGALVRCLSVNPQLDPTTLDGILLSHKHLDHSGDINIMIEAMTEGGYRRKG
                     VLFAPEEALEQDPVVFRYIRTYLGKIEILRENKTYSVGDLKFSTAARHHHSAETYGFN
                     FHLPEATVSYITDTKFHPELISQYPGEVLLLNVVLLAELPDRKIDHLSFEDVKIILKE
                     TRAKTVVLTHFGTRMLQANPSELTQRLSEETGKKIIAARDGMTLVVDEIV"
     gene            7082..7822
                     /locus_tag="VEL68_21585"
     CDS             7082..7822
                     /locus_tag="VEL68_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38637.1"
                     /translation="MGSGPNIVRNVLLTFAIGFFAAISPFLFMQLLPALLNPSLHPDP
                     PNYLAIILTGVLIGAITSILFANQFEKKGPQDIFFYALGIPAILIATVSNLSTEYKAV
                     GQVSEVRESLISAVLSPPKPEVLQEMPKPVPPPQTIKQGWDSRGNAWAQERQEGRPLL
                     LTQALNYFVVIGEYDNEKAAWDDYKKWKEKRLRTELYFPKDLGVFEIRKGQYILVYSR
                     HSSPEEANKVFQLLRINDPGLNVKILKY"
     gene            complement(7869..>8171)
                     /locus_tag="VEL68_21590"
     CDS             complement(7869..>8171)
                     /locus_tag="VEL68_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38638.1"
                     /translation="DDFCKRGGRFGPHQFAETQDGVIIGGTLLYVDAGLRVVDISDLY
                     HPREVGFYIPAPVALKSARRTMAIRTNDADLDYRGLVYITDRAGNGLHILEFTGKK"
BASE COUNT         2030 a         2133 c         2273 g         1735 t
ORIGIN      
        1 aagcccgagc acggacggcg aggctgaatg aatggcttac cctgggggcc ggagtcccgg
       61 gctgcatgca cggcggcggg tctccggacg gtgcccgtct ggttgtccgc tccctcctcc
      121 ccctcgaaaa atacgcccag atggccagga agctaggcgg aattaaagaa gaaatacccg
      181 accccggcaa atgaaaaaat agtgggaagc agattaaggc agaaaaacag gattaaaggc
      241 tttgtaattt ttaaggggtt ttatcggcat tgatcggtga aaatctgcgg ccggataaat
      301 gaaaggaggg cattaaaatg cctaacaaag tcgggcaggt ctacgtctgt gaaatctgcg
      361 ggaataaggt ggagatggtg gtcgccggaa agggaaccct tcattgctgc ggccagccca
      421 tgaagctggc aggagagaaa aaataggccc caaaacgaaa aaccttgact tttctctaaa
      481 aatccattaa aaaaaagcaa cttattattt cggctaggag ggcagactct cccaagaaag
      541 gagaaagggg cgatggacgg gtatgcggga aaaattctgc gggtcaacct cacaaccaga
      601 aggtgcaccg aagaacccct gaatccaaag tacgccaaag attacatcgg cggaaccggt
      661 ctgggagtcc ggatgattta cgatgaagtt cccccatgta cggaccccct cagcccggaa
      721 aacaaaatcg tctttaccgt cgggcctgtg accgccacca actacccttc cggcgcccgg
      781 taccagatcg ttttcaaatc tccactgact ggaattcttt gcgacgccag ctcgggagga
      841 tactgggggg ctgacctgaa acgggcaggc tacgacgcgc tgatgattga aggagccgct
      901 gacgacccgg tttatctctg gattcacaac ggcaagacag agatccggcc cgcaacgcat
      961 ctatggggtc aggatgccct gaaggttcaa gaaagcattc agcgggagat tggggacgag
     1021 agaattcggg ttgcctgcat cggcccggct ggggagagga aagttctcct atcctgcatc
     1081 atcaatgacg aaggacgcgc tccgggacgc ggaggaaatg gagctatcct ggggtctaaa
     1141 aatcttaaag ccattgccgt ccgcggtaac ggaaacttcc ctatccatga ccttgccact
     1201 tacaacactc tctgcaagaa gatcgccaag gataacgcta tttccccggc ggtggccgcc
     1261 atgcgggaat atgggaccgc tcaggtcctg gacaaccttt gggccatggg cgatatcccg
     1321 gtcaaaaatt ggcagctggg catttgggag gaggggtgca agaacctggg cgggaagaag
     1381 atgaaggaaa ccatcctggt tcctcacact gcctgctacc gttgcaccct ggggtgctcc
     1441 cggtgggtca agatcgaaga gggcccctac aagatggacg ggccggggcc ggaatacgaa
     1501 accctggcgg ctatgggaac catgtgcctc attgatgacc tcaacgcggt ttcctttgct
     1561 aacgacctct gcaaccgata cgggatcgat acgatttcca ctggggtggc catcgccttc
     1621 ggcatggagg cttacgaaaa gggcctgatc aagaaggagg acaccggcgg gatcgagctg
     1681 aagtggggcg atgcccaggc gatgattgcc atgacccacc agatcggaaa gaatgaaaag
     1741 ctgggagcac ttctggggca gggcgtgaag agggctgcgg agaagctggg cggagattcc
     1801 tggaaatacg ccgtccacgt gaaagggatg gaaaccgcca tgcatgatcc gcgtactttc
     1861 tacagcatag gactcaccta tgcggtggga ccacgcggcg cctgccacct ccacggccat
     1921 tctcctctat gggaaaatgt gcaggatccc cttcccgagt gggggctcaa aggaatgtat
     1981 ccactgcatg tgagcaaggg aaaaggactc gtggcaaagc tttcccagga ctataccgca
     2041 gtcgtcaatt ccatggtttc ctgctacttc ttgaccttca tcctgaaacc ctccgacctg
     2101 gcggccgtct tgagttcggc taccgggacg aagtacacgg ccaagagtct gctgaaagta
     2161 ggagaacgga tcatggccct ccaccgggct tacaacaacc tgtgcgggat cacccggaag
     2221 gacgatgtcc tagcaccacg ccagttggag gcgaccaagg aaggaggcaa tgccgggaaa
     2281 gttccggaca tggaggttat gctcaaggaa ttttacaaag tcagcggctg gactcccagc
     2341 ggcaagcctt cccgcaagac cctcgaatcc ctgggcctcc gggacgtggc caaggatctt
     2401 tacgggccga aggggaaata gcaagtacag acctttcctg ccggttcttc tacctcttgc
     2461 cttttcccaa ttgattcacg cgatgtagag gcgacccgcc gggtcgcctc tacatgttta
     2521 caattcagca cttccccgct actattgatt tatattgtct gcgctggagg taaaggtggc
     2581 agaagagaag aaatttacct atcccgcggg caagctccct ctggaagacc tctcccggct
     2641 cctctctcgt tttgcccgga acgatccccg gattgtgatc ccaccgggga ttgggaagga
     2701 tgccacggtg atctctttcg gggacaagta ccttgtggcc aagaccgatc ccatcacctt
     2761 tgccaccgac cagatcggct ggtatgcagt tcatatcaac gccaacgatg ttgccgccat
     2821 gggaggtgtc ccgcagtggt ttctggccac cctgctcttg cccgcaggga aaacaggtcc
     2881 cgaggaggtg gaggaaattt tctcgcaggt ctcaacggcc tgcgaggaac tacggatcat
     2941 tctttgtgga gggcacacgg aaatttccta tggaattgac cgtcccatcg tggtgggcca
     3001 gatgctgggg gaagtggaga aggagaaatt ggtctctccc gataagattc gatcagggga
     3061 cgagatcatt ctcacgaaag ggatcgccat tgaaggtacg gccctgatcg caagggaatt
     3121 aagggagata aattctctgc tggaggaaaa gagattggac cggtgccgga atctgctgcg
     3181 ctccccgggg atcagcgtag tgcgggaagc acggatcgcc aacgaagtgg ctgcggttca
     3241 tgccatgcac gacccgacgg aaggaggtct ggctacaggg atccaggaga tggccaatgc
     3301 cgccggtatg gggatgcggg tggaaatgga tcagattcat atcttgccgg agacggcaat
     3361 tctttgcgaa aaactcaagc tgcaccctct gggccttttg gcctcggggg ccatgctgat
     3421 tgctgtggcc ccaaaagact cgacaaaagt aatccaggct ctggagaaag aggggatttc
     3481 cgcatcggtg atcgccaagg tctgggaaag ggaaaagggg gtcaaattgg cggaaagggg
     3541 gaaaatcacc gatctgccct tttttgcaag agacgaactg gcccggctgt ttgatgggag
     3601 caagggatga gttcatttac tacccggtac agaaataaaa aattcgtcaa ggaaagggtc
     3661 gagctggaca ggcatgccta cgagaactgt gaattcgaag gctgtatgat tgtcctggaa
     3721 aaaggggaga ccgagatcaa ggggtgtaaa ttcaaccact gccgtttggt acttctgggc
     3781 caggctttgc gaatcgccaa ggtgctctcc ctcttcactg gcggcaagcc cctgaaggtc
     3841 ctggatttcg ccgaaccggg ggttttcggg gggaaaaaat aggattcacc gcagagacag
     3901 gttacaaaag aacaagttct ggagatttag aatcttttct tgcccactgg attcaaagcg
     3961 atttgcagct ctgtataatc tgtggtggaa aattaaaggt aaaaaatgat cattaaaacc
     4021 tactgtgccc ggatggacca cggagggtgc ggcctccggg tccatgtgga agacggaaag
     4081 attgtgaagg tcgaggggga tcaggattca cccctgaatc gggggacagt ctgcggcaaa
     4141 gggatcgccc aagtggagcg gatcaatcat cctgaccgtc tgcgccatcc cctgaaaaga
     4201 atcggggaaa ggggagaggg aaagtgggaa tgcatctcct gggaggaagc cctcacgacc
     4261 ctttcccaaa ggttcctcca ggctgaccgg cgaagtatcg cctttgccca gggaactccc
     4321 aaaggcctgg aactctttat gatgatccgc ctggccaacc tgctgaaagt cgccacagtg
     4381 gtcacccccg gttccatctg tcacatgcca agggaaactg ctgccatgat cacctgcggg
     4441 ttcttcccgg tgccggacta tgatcatcct ccggctctag tgatcgtatg gggaagcaac
     4501 ctcttccaga ccaatgagga ggggatcatt gggtctcaac tgcgcagggt tctggaccag
     4561 ggagccaagc tgattgtcat agaccctcgg aggacccaat tgtcaactcg agcggacctt
     4621 tggctccagc ccaggcccgg tacagacctg actcttgcgc tcgggatgct gaaagtgatc
     4681 gtggatggcg gactgcatga ccgggccttc gtcgatcaat ggaccgttgg ctttccggag
     4741 ctgcaggaac atttaaagga ctattcgctg aaaacgctgt ccgaaactac ctgggtcccg
     4801 gaggaaaaga tcaccgagac agcccgtttc tatgcccgta tgcgaccggc ctgcatccag
     4861 tggggaaacg ccatcgagca caccattcaa agcgtgcaga cggcccgggc gttgatgatc
     4921 ctgaaggcca tcaccgggaa cctggattca ccgggtggaa atgtgaatcg cccttctcct
     4981 ccgactttgc gcccgggaga ttttgtacaa ggcaagagct tccctgaaaa gcgggaggag
     5041 cttctggctc cggagtttcg gctagctgcc atgatgggat tcacccccag ccagaccgtg
     5101 gtgaaagcca tcctttcggg caagccttat cccatccgga tgatgtacat ccagggagga
     5161 aaccccctct tgagttatgc caacgccacg gagacttacc tggccctgaa gaaactcgat
     5221 tttctagcgg tggccgaaat cttcctgacc cccactgcgc agcttgccga catcgttctt
     5281 cccacggcga cgaactttga gttcgacgat atgggacact atgggatgcc ccacggcttc
     5341 ctcctggccc ggcccaaagt cgtcgaccct ccgggagaat gcctgccgga tttgctcatt
     5401 ctgaacgatt tgggtaagag gtgcgggctg ggaaaattct tctggcccga tctccgctct
     5461 tgcttagatg aggttttgaa acccgcggga gtcaactatg aagctttcaa ggaagcggga
     5521 atgctgaaag ggaattggaa gtacaaaagt tatgagcgca gcggcttcgc cactccatcg
     5581 aagaaagtgg agattttctg ctctcgtctg aaagattggg gatatgatcc cctcccgacg
     5641 tatcgccagg aattttcggc gcccgacagc gatccccatc ggatggagaa ttatcctctg
     5701 attctcacct cggccaagga cccctgttat ttccactccg cctaccggaa tcttccttcg
     5761 ctaagaaaat tctcccccga cccggtggtt ctattgcatc cgcaaacggc ggagggcatg
     5821 ggcgtcaaag agggagactg ggttcacatt gaaacccagc ggggaacgat ccgccagaag
     5881 gcagctttcc atgccgagct cgatccccgg gtcgtggtcg cagcctacgg gtggtggttt
     5941 cccgaaagaa aagacctgga actcagtggc tggaaagagt cgaacatcaa cattctgacg
     6001 gaaaatgatc cgccctatga cccgaccatc ggatcaacaa atctgagggc tattccctgc
     6061 cgtgtctata aggaataagg aacgtatcaa agaggaaaaa agaggaggtt caaggagaat
     6121 caataaaaat ataaatgggg aaaaagtttc tcccagttgg cgcagagaaa tcaaaccgtt
     6181 gcagatttca actagcaagt ttcaggttat atgaacccgt catcagaggg aaaatcggct
     6241 tacaagaatt ggattaagtt tatcggtacc gccggcgctc gcttcgttgt ggcacggcag
     6301 ctgagggctt caggcggcct ctggctgtct ttccagggag ccaatctgta catggacccc
     6361 gggccgggag cgctggtccg ctgcttgtcg gtaaatcccc aactcgaccc cacaaccctg
     6421 gatgggatcc tcctttccca caagcatctc gaccattcgg gcgacattaa tatcatgatc
     6481 gaggccatga ctgagggggg ctatcggcgc aaaggcgtgc tcttcgctcc ggaggaagcc
     6541 cttgagcaag accctgtcgt ttttcgctat attcggacat acctgggaaa aatcgagatt
     6601 ctcagagaga ataaaacata ttccgtgggc gacctaaaat tctctaccgc cgcgaggcat
     6661 caccattcgg ctgaaaccta tggctttaac ttccatcttc cggaggctac cgtctcctac
     6721 attaccgaca ccaaatttca cccggaactg atatcccaat atcccggcga ggtgctcctt
     6781 ttaaatgtag tattgcttgc cgagcttcca gaccgcaaaa tcgaccacct ctcttttgag
     6841 gacgtgaaaa tcattctcaa agaaaccaga gccaagactg tggtccttac ccattttggg
     6901 acccggatgc tccaggccaa tccttcggaa ttaactcaac gcctaagtga ggagaccggg
     6961 aaaaaaatca ttgccgctcg ggatgggatg accctggtgg tggatgaaat agtctaaagg
     7021 gcgtcaagag ttatccagta gccatttcca aaggttttct gaaccaggaa aggaggaatc
     7081 catgggttcc ggtccaaaca tagtccgaaa tgttcttttg acttttgcga taggattctt
     7141 tgccgccata tcgccctttc ttttcatgca acttctgcca gccctgttaa acccgagcct
     7201 tcacccggac ccgcctaatt atttggctat tattttaacc ggcgttttaa tcggcgctat
     7261 cacttccatc ctcttcgcca accagtttga aaaaaagggg ccgcaggata tatttttcta
     7321 tgccctgggg atccccgcca ttcttatcgc cacggtgagc aatctaagca ccgaatataa
     7381 agcggtgggc caagtctcgg aagtgagaga gagtttgatc agcgcggttt taagcccacc
     7441 taaacccgaa gtccttcagg aaatgcccaa gcctgtccca ccgccccaga ctatcaagca
     7501 gggctgggat tcaaggggaa acgcctgggc gcaagagaga caggaaggta ggccccttct
     7561 tctgacccag gctttgaact actttgtcgt catcggtgaa tacgacaatg agaaagcggc
     7621 ctgggatgat tacaaaaaat ggaaagaaaa aaggcttcgg acagaactct atttccccaa
     7681 agacctaggt gtatttgaaa tccgaaaggg gcagtatatc cttgtgtact cccgccattc
     7741 atcgccggaa gaagccaata aggtttttca gttgttaagg atcaatgacc caggattgaa
     7801 cgtgaagatt ttaaaatact gaaaggaagg cctccttccc aggctttctc ttggctgaag
     7861 gctgacggct atttcttccc tgtgaactcc agaatatgca gtccattccc cgcccggtcg
     7921 gttatataaa ccaatcctcg ataatccagg tccgcatcat tggttcggat ggccattgtt
     7981 cttcgtgcgc tttttaaggc caccggggcg ggaatataaa agcctacttc ccgtggatgg
     8041 taaagatcgg agatatccac gactctcaag ccagcgtcta cgtaaagcag ggtccctccg
     8101 atgattactc catcctgggt ctccgcaaac tggtgggggc cgaagcggcc gccccgtttg
     8161 cagaagtcat c
//
LOCUS       DASPRR010000588         6471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_458971, whole genome shotgun sequence.
ACCESSION   DASPRR010000588 DASPRR010000000
VERSION     DASPRR010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458971"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VEL68_21595"
     CDS             complement(<1..252)
                     /locus_tag="VEL68_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ38639.1"
                     /translation="MSARGPIPDYRFQPMEKGDLAEVIQFWQGMEGIGLSESDSYSAL
                     SFYLQRNPGLSWVTRNKDRELIGAVLCGHDGRRGYLHHLA"
     gene            complement(249..947)
                     /locus_tag="VEL68_21600"
     CDS             complement(249..947)
                     /locus_tag="VEL68_21600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019217543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXZ38640.1"
                     /translation="MILRLMVTALFILAAGSAGAQDKPSLKDQKEKISYIIGMDIGAN
                     FKKQSINIDPDIVAKGIKDGLSGAKPLLSEPEAREVMTAFEKEMRAKQEAALKVAGEK
                     NKKEGEVFLAENKKKEGVKTLPSGLQYKVIKPGTGKKPAATDTVTTHYKGTLIDGTEF
                     DSSYRRGKPATFPVSGVIPGWTEALQLMEEGAKWQLFIPPNLAYAERGAGQVIGPHAT
                     LIFEVELISIQEKK"
     gene            complement(1492..2694)
                     /locus_tag="VEL68_21605"
     CDS             complement(1492..2694)
                     /locus_tag="VEL68_21605"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HXZ38641.1"
                     /translation="MNFLGYRRPDGSVGVRNYVLILPATRLCNQVAYRIGQTVAHTKT
                     VISTGEYGRHKRDRDRQARVLVGLARNPNVAATVLIGMKEGVGYPEFQLSRLVQQIAS
                     CGKPLVAVNLSEEGGMHRAIGKGIIEARRLVYEASKIRREPFPLEKMIIGVKCGSSDP
                     TSGISGNPAFGNLVDRVVGAGGTALFSETIEVVGAEDELAKRAVSPKVAEDLYGAVKK
                     TEKAAAGIGEDIRTINPIPENIAAGITTLEEKSLGAIAKSGTVPLQGVLEYCQQPPGK
                     GLYFMDGWMSSFSLPMSFAAAGCQMVFYQIGGQGMPDEDPPIYATNTGVVAPLIYVTG
                     NPRTYQKARLHIDMTTGEVLTGEMSVSQAGERLLNLAVDIASGMLSKGETVNYMDPLE
                     VYFEGPFL"
     gene            complement(2691..2999)
                     /locus_tag="VEL68_21610"
     CDS             complement(2691..2999)
                     /locus_tag="VEL68_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HXZ38642.1"
                     /translation="MKFQAIALHEKDNVGVLLEDVKKNQCVPIGGRQKHEIAARDDIR
                     FGHKIALMDIPQGGIIQKYGHPIGVATQKIFAGEHVHIHNVGGLRAGGKSPAGKGERA
                     "
     gene            complement(3042..3830)
                     /locus_tag="VEL68_21615"
     CDS             complement(3042..3830)
                     /locus_tag="VEL68_21615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ38643.1"
                     /translation="MPKIAVRDIEVNYEEAGSGYPFILVHGLNGDLTGWVLIMPELSK
                     YYRTVAMDVRGHGGTSKPDHSYSIKGFSEDLYEFFKKLQIPKGHIFGFSMGGAVAQQF
                     ALDHPEMIRSLILVSTFSHIDDHTRRAFAGLKASLARGGYPAFFDEVVHLAFTPKYIA
                     ANPGPIAELRKKRIAINSPVAIGRATDACLAFNLKDQISKIMIPTLVVSGREDVFTPI
                     HLAEQIHRSIHGSEWKILEGVGHNLPIESAPGLVRAVLDFLSRN"
     gene            complement(3921..4466)
                     /locus_tag="VEL68_21620"
     CDS             complement(3921..4466)
                     /locus_tag="VEL68_21620"
                     /inference="COORDINATES: protein motif:HMM:NF021561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HXZ38644.1"
                     /translation="MNSVAKKGIEKLAMAHGLKDFKWMDPRRMIIGEWVRMKCNYGCP
                     GYGKRKTCPPYVPSVEECTRFFKEYRHGLFFHFAKKFKDPEMRHPWSKEVNQRMLDLE
                     RKVFLSGYQKAFVFPQAPCRLCKNCTADSQECRQPYQSRPTLEAFGVDVYSTARMLGY
                     PIQVVKDYHDEMNRYGMLLVE"
     gene            complement(4674..5666)
                     /locus_tag="VEL68_21625"
     CDS             complement(4674..5666)
                     /locus_tag="VEL68_21625"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXZ38645.1"
                     /translation="MKWVTFVAKGSKDRQPQVGALLDDHSVLSLQPAAALYLKEVEKE
                     KDPFSMAARLAPKDMVTFLERGKTAMNLARRTLKAVAQWQKTEKKVLKGLRKEPLIFP
                     LAKVLLKAPVPRPGKIIAMGLNFRDHAEENKVPIPELPVGFLKASSCVVGPYDPVPYP
                     HDSTKQLDYEIEMAIVVGKKGKNIPKEKAYEYVAGYTIINDLSARDIQHKEMEKRLVL
                     LSKSLDAFGPMGPWLVTPDEIPDPHNLGIELWVNQEPEPRQKSSTNQLIYKVPDLIAY
                     WSQMTLEPGDVITSGTPGGVALFRQPDPQQWFLKPGDVVEARIEGLGAIRNMIV"
BASE COUNT         1334 a         1861 c         1636 g         1640 t
ORIGIN      
        1 cgccagatga tgaagatacc ccctccggcc gtcatgcccg cagagaacgg cgccgatcag
       61 ctcccggtcc ttatttcgtg taacccagct aaggccggga ttccgctgca gataaaagga
      121 gagggcggaa taggaatcag attcgctcaa tccgattccc tccatcccct gccagaattg
      181 gattacctcc gctaggtcgc ctttctccat gggctgaaac cgataatcag gaatcggtcc
      241 gcgggcgctc atttcttctc ctgaatagag atcaactcga cttcaaaaat caaagtggca
      301 tgggggccga tgacctggcc ggccccgcgt tccgcgtagg ctaagttcgg aggaataaag
      361 agctgccatt tcgcgccttc ctccatgagc tgcaaagcct ccgtccaacc cggaatcact
      421 ccgctcaccg gaaaggtagc cggctttccc cgccggtagg agctatcaaa ctcagttccg
      481 tcgatcagag tccccttata atgggtcgtt acggtatcgg tagctgcagg cttctttccg
      541 gtccccggct tgatcacctt gtattgcagc ccgctgggca gggttttcac tccctctttc
      601 tttttatttt ccgccaggaa gacctctcct tcctttttgt ttttctcccc ggctactttc
      661 agggctgctt cctgcttggc cctcatctct ttctcaaaag cggtcatgac ttcccgggct
      721 tccggttcgc ttagaagcgg cttggctccg gaaagcccgt ccttgatccc cttggccacg
      781 atatccgggt cgatgttgat cgattgcttc ttgaaatttg ccccgatgtc catcccgata
      841 atgtagctga tcttctcctt ctgatccttt aggctcggct tgtcctgggc ccctgccgaa
      901 ccggccgcca ggatgaataa tgccgtcacc attagtctga gaatcattct aactcctttc
      961 cctattcgaa ttcgctgctc caaaatctct ggcaacaaaa aaccccgctc cggaatcaga
     1021 aacggggttt gctcctcctg catgaaaagc cctcgactct ctgaaggggc gataaggctc
     1081 ctaatttttt ataccaccaa acctccgaaa catcaatcgg aagactcctt tccggttggc
     1141 cacgactctg gaacttttcg gcaaaggccg caccgggagt cggaacacca ttgacagaaa
     1201 tagcaatccg ggcagttgtg ttttttcgcc ctggtggcct tagggcaata gaacattccc
     1261 tgggcggtta ccgcgaaccg attcccatcc cggtcgatga attctttttt cttggaagaa
     1321 tttccttttt tgtccttaac cttatgcttt ctgtccttcc cttccttttc ttgcctgttt
     1381 cgcattctga attccgccct gcaccacata ggattcaaag ccttgccctg aactacatgg
     1441 gattcagggt cgtgccctga accttacatg gttcagggcg cattccgcat tttacaaaaa
     1501 tggcccctcg aaatagacct ccaggggatc catataattc accgtctccc ctttgctcaa
     1561 catgcccgag gcgatatcca ccgccaggtt gaggagccgc tctcctgcct ggcttacgct
     1621 catctcccca gtcaggacct ctccggtggt catgtcgata tgcagtctgg ctttttggta
     1681 ggtacgggga tttccagtaa cgtagatcag gggggctacc actcccgtgt tcgtggcata
     1741 gatggggggg tcctcgtcgg gcatgccctg ccctccaatc tggtagaaaa ccatctggca
     1801 gccggccgcg gcgaaactca tgggcaacga gaaagaagac atccagccgt ccatgaagta
     1861 caatcctttt ccgggaggct gctggcaata ctccagaacc ccttgcaaag ggacggtccc
     1921 gctcttggcg atggccccga gggatttttc ctcaagagtg gtaatccccg cggcgatgtt
     1981 ctcggggata gggttgatgg tgcggatatc ttctccgatt ccggccgccg ctttctcggt
     2041 ctttttaacc gccccataaa ggtcttcagc caccttgggg gatacggctc ttttggccag
     2101 ctcgtcttcg gcgccgacca cttcgatggt ttcggaaaaa agggcggttc ccccggcccc
     2161 caccacccgg tcaaccagat tgccgaaggc cgggttcccg gaaattcccg aggtggggtc
     2221 cgagctgccg catttcaccc cgatgatcat cttttccagg gggaaaggtt cccgccggat
     2281 tttcgatgcc tcgtaaacca gccttcgggc ttcgatgatt cccttgccga tggccctgtg
     2341 cattccgcct tcctccgaca ggttgacggc caccaggggc tttccgcagg aggcgatctg
     2401 ctgcactaat cgggaaagct ggaactcggg gtacccgacc ccttccttca tcccaatcag
     2461 gaccgtggct gccacgttgg ggtttctggc cagccccaca aggaccctgg cctgccgatc
     2521 ccggtctcgt ttgtgtcgcc cgtattctcc ggtggaaatg actgttttgg tgtgggccac
     2581 ggtctgcccg atccgataag cgacctggtt acacaggcgg gtagcaggca ggatcagaac
     2641 gtaattccga acccccacag aaccgtccgg ccttcgataa ccgagaaaat tcatgcccgc
     2701 tctcctttcc cggccggcga ctttccccct gcccgcaggc cgcccacgtt gtgaatgtga
     2761 acatgctcgc ccgcgaaaat tttctgggtc gccactccta tggggtggcc gtacttttgg
     2821 atgatccctc cctgagggat atccatcagg gcgatcttgt ggccgaagcg aatatcatcc
     2881 cgcgccgcaa tttcgtgttt ttgccgtccc ccaatcggaa cgcattgatt cttctttaca
     2941 tcctccagaa ggacgcctac attgtccttt tcatgaaggg cgatcgcctg aaacttcatc
     3001 ttttcctccc ctcaaacggt ttgaactgaa aaggctcggt ttcaatttct tgacaaaaaa
     3061 tccagaacgg ctcgaaccag acccggggcc gattcgatgg gtagattatg ccccacccct
     3121 tccaggattt tccattcgga cccgtggatg gaacggtgaa tttgctcggc aagatgaata
     3181 ggagtgaaaa catcctcccg gccggaaacc accagggtcg gaatcattat ttttgaaatc
     3241 tgatctttca gattgaatgc taggcaggca tccgtggctc ggccgatggc caccggggaa
     3301 ttgatagcga tccgcttttt tctcagctcg gcaatggggc cgggattggc agcaatatac
     3361 ttgggagtga aggccagatg aaccacttcg tcgaagaagg ccggataacc tccccgggcc
     3421 aggctggctt ttaaaccagc aaaggcccgc cgggtatgat catcgatatg gctgaaagtg
     3481 gagacaagga ttaaggagcg gatcatctcc gggtgatcca gagcgaactg ctgggcgacc
     3541 gccccaccca tggaaaagcc gaagatgtgg cccttcggaa tctgcagctt tttgaaaaac
     3601 tcatacaggt cctcggagaa tcccttgatc gagtacgaat ggtccggttt gctggttcct
     3661 ccatgccccc ggacatccat agcgaccgtg cgatagtatt tggacaattc gggcatgatc
     3721 aggacccacc cagtgaggtc cccattcaga ccatggacca ggatgaaggg atacccgctc
     3781 cccgcctctt cataattgac ttcgatatcc cgaacggcaa ttttcggcat cccgttatct
     3841 ccccggcgca aaattcgaga ctctgttatc ttcccatatg ttcaactcgg aatttgaaac
     3901 tcgaaacccg taacgtacat ttattccacc aagagcatcc cgtaccgatt catctcgtcg
     3961 tggtaatcct tcaccacctg gatgggatat cctagcatgc gggccgtact gtacacatcc
     4021 accccgaaag cctccagcgt ggggcgggac tggtacggct gtcggcattc ctgggaatca
     4081 gccgtgcagt tcttgcacaa ccggcagggg gcctggggaa atacgaaggc cttctgatag
     4141 cccgaaagga agactttcct ttccaggtcg agcattctct ggttcacttc cttcgaccag
     4201 gggtggcgca tttccgggtc tttgaacttc ttggcgaaat ggaagaatag gccatgtctg
     4261 tattctttga agaatctcgt gcactcttcc accgaaggaa catagggagg gcaggtcttc
     4321 cttttcccgt acccgggaca accgtaattg cacttcattc tcacccattc tccgatgatc
     4381 attctcctgg ggtccatcca cttgaaatcc tttaatccat gagccatcgc cagcttttcg
     4441 atccctttct ttgccactga attcatcttc ccctcatttc tttttgtcgc ccaccgagca
     4501 ctccaaggcc tcggattaac aacgattcaa ataaaaattt tatctgtttg ctcctaggcc
     4561 ttggggggtt cagattttgg atattttctt tacggctccg tgatctctgc gccctcggtg
     4621 gttaattttt ttgattgcag ttctgctccg ctgtggtctc tgcggttaaa attttagacg
     4681 atcatattcc gaatggcccc caatccttcg atccgggcct ccaccacatc ccccggcttc
     4741 aggaaccact gctgcgggtc tggctgcctg aacaaggcga ctccgcccgg ggttcccgag
     4801 gtgatcacgt cccccggctc cagggtcatc tgggaccagt aggcaatcag gtcgggaacc
     4861 ttgtagatca actgattggt ggacgatttc tgcctcggct ccggttcctg attcacccag
     4921 agttctatcc cgaggttatg cgggtcggga atctcgtccg gggtgaccag ccagggtccc
     4981 atggggccga aggcatccag actcttggac aagagaacga gcctcttctc catctccttg
     5041 tgctggatat cccgggcgct caggtcattt atgatcgtgt aaccggcaac gtattcgtag
     5101 gctttttcct tgggaatatt tttccccttc ttccccacca cgatggccat ttcgatttca
     5161 taatccagct gtttggttga atcatgagga tagggaaccg gatcgtaagg acccaccacg
     5221 cacgaggagg ccttcaaaaa tcctacgggc aattcgggga tgggaacctt attctcctcg
     5281 gcatgatcac gaaaattcag ccccatggcg atgatcttgc cggggcgggg aaccggggct
     5341 ttcagaagga ctttcgccag ggggaaaatc aagggctcct tgcgcaggcc cttaaggact
     5401 ttcttttctg ttttttgcca ttgggccaca gccttaaggg tgcggcgcgc cagattcatg
     5461 gctgtcttac cccgttccag gaaggtaacc atgtccttgg gagccagccg ggccgccatg
     5521 gagaaagggt ctttctcttt ctccacttct tttaaataaa gggctgctgc tggctgtaag
     5581 cttaataccg aatggtcgtc gagaagagcc cccacttggg gctgtctgtc tttgctgcct
     5641 ttcgcaacaa aggtcaccca tttcatggtt tccctcctta aaagattttt ttagggcgtg
     5701 aaaaggacaa taataaaagt ttactaacat gcatgaaggg agtcaacaaa gattgattgg
     5761 gtatttgagg gaaaggctgt cggtcttcgc ttaaagaaga aggcctgact tttcagggga
     5821 attcccaagc aagaggtccg gtcgtttctc ttgagtcggg ggaagcagac gccattgggc
     5881 aagaatctat ttcaataccc ggactaattg cgcagtgccg taaggtccag ctcgtgcaga
     5941 gatacttacg gggttggggc tgttttgact gttgggggtc tgtccaggtt tctgttcctt
     6001 ccggccggac ggaaagcgtg agggcccgtt tcccgacggg tttcagggtt gggcataagc
     6061 agtccgatcc ggcctttcat ggcgggcggg gtcattggaa gagccctggg cagaccccgg
     6121 agactttctt ccaacatctc tcgacgattc tttctatccc ttcgcgagga tcttcaataa
     6181 agtcttgaag gtcgattctg gatacccctg aggactgagg atacggctta cttccctggg
     6241 tcgattcatg ttttcctgcc tgggcggaaa agctggagcg aacgcggatt tcccaatacc
     6301 aaaaggcgcg gcctcctctt ggccttgagg aggaagaggg gcaggaacaa taaaaccaag
     6361 ttttcgatca ttccgctctg gcatcttttt tcctccttct tttctatccg gccatccttc
     6421 gcgattcggg agagtcaagc aaaactccac cctctccgcc gtccatgaac t
//
LOCUS       DASPRR010000589        14085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2081270, whole genome shotgun sequence.
ACCESSION   DASPRR010000589 DASPRR010000000
VERSION     DASPRR010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081270"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VEL68_21630"
     CDS             complement(<1..212)
                     /locus_tag="VEL68_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38646.1"
                     /translation="MINNFEGSEKSRREAVIKAILFLAFLAGALYIIRFTRAKEFFTP
                     HELNRFLEEAGVWAPLAYILIYAVGV"
     gene            463..1479
                     /locus_tag="VEL68_21635"
     CDS             463..1479
                     /locus_tag="VEL68_21635"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ38647.1"
                     /translation="MTGREIAQMAFDLKKPPRVPVTLIGGGAWAVHHAGKTFGQIKND
                     PKQIADVFIRFFRLFQNDLIWTGSNFLNYPVHFLGCPIKDDSADSPGLEGTVIQSLEE
                     IDRLKIERVFQNPTMQAIIRSHHLIADALGRETMIVPTQWAPFTLTARILGMEPLLIA
                     TIQEPEKLSRLIRFSTELTWALLEPILEHPDIPGANFSDPVASGDVVSPSTFRKFSAP
                     AIKELVNRVKAKKKYSMVHICGNSTQVLKDVLEINPHCFSLENKVDLKAAREILGGKV
                     CVAGNVSPTGAFLSGRPEQVQEEAKACLAAWGDGGGYILTVGCDFSKNVPMENMKALM
                     SFRK"
     gene            1581..2744
                     /locus_tag="VEL68_21640"
     CDS             1581..2744
                     /locus_tag="VEL68_21640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ38648.1"
                     /translation="MLLLFVLAHFCHHLLVALTIPLSPMIRSDFSLDYTYTGLVISAF
                     NLAYGIGQLPGGWMADRFGPRRMITLGIWGVALAGFIVGLSQNFWMMVVFLALMGILG
                     GGYHPAAAPMISSLVEPPRRGRALGLHMIGGGASFFLSPLIAMAIAAYWSWRGSFIGL
                     AVPTLAFGIYFHLLLKKREAGEPLGQVRIPRPSEKIPKQRLLGPLIVVIFLSTFSAAV
                     LFSVISFIPLFLVDKIGYSKETAGTFYALIYSAGLWASPMGGYLSDRFGPIPVLLFAL
                     FLTGPVIFLLHFVSTWLGIGILLLSLGMIIYVRMPVSESYILSNTPEGRRSAVLGVYF
                     FAGMEGGGILAPVMGYMIDHLGFFASYTFAASIIILTTLVCGILLRGNLKWKP"
     gene            2906..5140
                     /locus_tag="VEL68_21645"
     CDS             2906..5140
                     /locus_tag="VEL68_21645"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXZ38649.1"
                     /translation="MGNDCLAELNPFLSKNALTVLEKRYLIRDNGKVLENPTDMFRRV
                     AEAIASPDKKFDSQADLGKIAGEFCRLMAGLEFLPNSPTLMNAGRELGQLSACFVLPV
                     GDSMEEIFDAVKYTALIHKSGGGTGFSFSRVRPANDVVRTTSGVSSGPISFMKVFDMA
                     TETIKQGGTRRGANMGILRVDHPDILNFIQCKTDQNHLTNFNISVGLTEAFMRAVERG
                     TPYSLINPRNGEAVGSLAAREVFDRIVKQAWQNGEPGIVFLDRLNRDNPTPHVGIIES
                     TNPCGEQPLLPYESCNLGSINLGLMAKEGRIDWDRLKEVVHLAVHFLDNVIELNKYPL
                     PQIAEMTHANRKIGLGVMGWADMLIALGIPYNSEEAIALAEKVMQFLQAEGHEASRRL
                     AKERGAFPNFKGSIYDQRGEPPLRNATVTTIAPTGTLSIIANASSGIEPIFAVSFVRQ
                     VLDNNTLVEVNPQFERIAREQGFFSPKLMKEIAEHGSIQDLEEIPAEVRRLFVTAHDI
                     TPEDHIRMQAAFQKFTDNAVSKTVNFSRHATVEEVREVFELAYRLGCKGVTIYRDGSR
                     DQQVLSTGRKAEETEGAADNGKKVARKRPMALKGWTYRMQTGCGPLYVTINEDENGLF
                     ELFTTMGKAGGCAASQSEAIGRMVSLAWRSGVHVNSVVKQLMDISCHSQSGFGENRVR
                     SCADAVAKAIQQHLSSNGPGEIEREEVEKAPLFKGACPECGGIVEHEGGCSVCHACGY
                     SECA"
     gene            5278..5703
                     /locus_tag="VEL68_21650"
     CDS             5278..5703
                     /locus_tag="VEL68_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38650.1"
                     /translation="MKTCAYCSELLDDGVMECPKCGRSMFIQARTKAPSAGFSLKEDL
                     SLPKRTLSDLHLGVLELLSAQPDFVNASQFIMSLPLENESQAQTAVMAIEEMVGAGLI
                     EVKGRSKIRISPKGKELYDREKDLPGPEQKTEWDADKRR"
     gene            complement(5894..6898)
                     /locus_tag="VEL68_21655"
     CDS             complement(5894..6898)
                     /locus_tag="VEL68_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HXZ38651.1"
                     /translation="MKRFWQHRRAILLAAFSFVFLVWLGLESEVFARAGGGTSSGTRS
                     YSSGGSTSRSTTPAQPSPYQQKQSQQTQPDPAVPPASPTRGLFSGLMGGMIGGLLGGM
                     LFSSLGFAGPGFGGEFGLADIFLILVILGIIYFVVKRFRYRQTLQMNMAGAGGSPYPY
                     PGPGPSLGPILGPPPEEEDYKEGPSPDAMRHIKAMDPTFNEKAFKDLAEDMFFKIQGA
                     WTRRDLKGIRSLLTPAMYEVFKEQVDRLIADHEINRLENIAVREVEIIEAGQNRGEEY
                     VTVRFYANLLDYTIQEKTGQIVAGSSSDPVKFVEYWIFTRNVGEKSWILGGITQEGDR
                     "
     gene            7023..8141
                     /locus_tag="VEL68_21660"
     CDS             7023..8141
                     /locus_tag="VEL68_21660"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXZ38652.1"
                     /translation="MKYSCSSCQRLFAISEKRFRCTCGGFLRVEYSGLFSKKDLERRD
                     HSIWRYREGFGLPEEVQPVSLGEGRTPIIGRTYEGADLTFKLEFLQPSGSFKDRGASV
                     LLSLAKSLGISHVVEDSSGNAGAAISAYAAAAGIRCTVYVPDYTPEGKLIQMKLYGSE
                     VVKVPGKRQEANRAAIEASEKSYYASHLWNPYFILGLQSAAFEIWEQYEGALPPRVIV
                     PVGGGGFLEGLYSGFAALRNAGYVKEVPRLIGVQAENCPPLHRAFHEGLPDYREMEVD
                     TTVAEGIAVQKPPRGKAVLQALRESGGWTVGVTEEEILSAEKALFSMGLFVEPTSAAA
                     LAAWRKLPLTEKDGSLIVLTGNGLKETQKLSNLFLGPS"
     gene            8316..10226
                     /locus_tag="VEL68_21665"
     CDS             8316..10226
                     /locus_tag="VEL68_21665"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HXZ38653.1"
                     /translation="MTHRERILKAIRGEMPDRLPFAPRLDLWYNANVQAGTLPERHKS
                     RTFDQIARAEGWALHKINPEYQKSRKAEDNLHWALGILSYKESVFGFRFSPDVDVEAK
                     ESGGRTKIIYHTPIGSVSTTTVYTEEMRKAGVSNFWVEEHVLKGPKDYRVVAYIFENL
                     KLFRDDRDFLKWKEEIGEDGVPFTLAGRAASPMHHIQKYFVDATDFYFHYHDYAKETQ
                     ALAESMGGFFDEIIQQVGDSQAEVVYWGANFDDMITYPAFFEKDIVPWIRKAADILDR
                     KGKYVSCHCDGENLGLMDLIRDSGMHIAEAITPAPMTKVPIEEYYRRWSDKLTLFGGI
                     PSILLLRESISDEEFEAYLDHLFKVIAPGRRMILGIADSTPPKAVFERLVRIGEKVEK
                     EGRLPLEAGGFRPLIQPVVPEAAKPVLREIVWDEECRQVQNDVLEGDQAAIQEHIQSL
                     LAKGVKAEEILKRGMLSAMEVISERFKSGEAFIPEVLLSARAMNEAVKVLQPHLGGTQ
                     LAGKGKVLIGTVKGDLHDIGKNLVITMLKGVGFETVDLGIDVPTPAFIKAVEEHKPHI
                     LGLSALLTTTMPQMQAVIEGLKQKGLRDRVKVMVGGAPVNEKFARDIGADSYAADGAD
                     AVTLAKKLIQQE"
     gene            10819..11196
                     /locus_tag="VEL68_21670"
     CDS             10819..11196
                     /locus_tag="VEL68_21670"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXZ38654.1"
                     /translation="MGKQISRRSFLKKGAIALGTAGVGDFKGIGSAEQVSSSKPLLGE
                     ESKETIDPHVIVKLYPGRSEQQKIRLAEQIVKDVVSIIKCGEESVSVAIEEIKAEDWA
                     EKVYKPDILNTPGKLYKKPGYSM"
     gene            11394..11888
                     /locus_tag="VEL68_21675"
     CDS             11394..11888
                     /locus_tag="VEL68_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009369269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ38655.1"
                     /translation="MKYIALLLSLSLLVSMGFFAGQALTGEAKKESQTISRAGSQPSV
                     KGSGEYFTGNVRIDPLFPAKDSAPFSGAYVTFEPGARSAWHIHPTGQHLLVTAGVGRT
                     GEWEGKVEEIKAGDVIWCPPKVKHWHGASPTTAMTHIAITGTVGGKNVEWMEKVTDEQ
                     YNGK"
     gene            11899..12630
                     /locus_tag="VEL68_21680"
     CDS             11899..12630
                     /locus_tag="VEL68_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXZ38656.1"
                     /translation="MDKKSTWFLAGIFILILGCATNSEAQTVNTQMLSPKQKSIVTIS
                     AFTANGDQEKLATALNEGLDAGLTVNEIKEILVQMYAYCGFPRSLNGISTFMVVMDER
                     QKKGIKDEMGNEVSPLPADMNKDEYGARVRAKLSGSDVVPPPSGYQLFVPIIDKFLKE
                     HLFADIFARDTLDYQSRELATISALASMTGTAGQLRFHLGAAMNTGFTETQMKDFISV
                     LNAKVGQKEAESANQVLTAVLQSRK"
     gene            12789..13181
                     /locus_tag="VEL68_21685"
     CDS             12789..13181
                     /locus_tag="VEL68_21685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXZ38657.1"
                     /translation="MNAKQFLILVASVLTLSLCGGALAEEMQGRYVRLAELEIDPAQL
                     ESYKGVAKEQIETSVRVEPGVLALYAVSQKDNPAHVIVFEMYTDADAYRAHLETPHFK
                     KYKAKTQDMVKSLKLVETVPIILSAKPK"
     gene            13295..>14085
                     /locus_tag="VEL68_21690"
     CDS             13295..>14085
                     /locus_tag="VEL68_21690"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXZ38658.1"
                     /translation="MIERREFLKKMALLAGVMGGGALLPASGKTAEQEIGIAVVEGEN
                     PKAQVQEAIRLLGGIQRFVRRGDRVVLLPNPQGTGRGVTTNPDMVAETVRLCLAAGVS
                     SVSVSSCHGREDWLGTGIIEKVEASGGRMKYPDSDRDWATISIPKARVRKEVKVIRDA
                     VQADVLINMPIFKQHNYTRVTGCLKNLMGTNDDNWSFHKGDLHLHQAIVDLASIFSPA
                     LCLVDATTILTQGGPFGPGRVIHPKKVYAGKDMVGLDALCCDLLNV"
BASE COUNT         3251 a         3868 c         3944 g         3022 t
ORIGIN      
        1 cagacaccta ctgcgtagat caggatgtag gccaaaggcg cccagacgcc tgcctcttcc
       61 aaaaacctgt tcaattcgtg aggggtaaaa aattccttag cacgggtgaa gcggatgatg
      121 tagagggcgc ccgctaggaa agcgagaaaa aggatcgcct tgattaccgc ctccctgcga
      181 cttttttccg aaccctcgaa gttattgatc atcagaaccc tcttcccccg ggagaggctg
      241 gaaaacctag aggagaatga aaacccaccc tcttccattt tccccggaat cttcttccag
      301 agcaatcagg attcctttgt attttgccca ggggaagacg gtattttgaa ccactcccgc
      361 cccccttccg atgaaaatcc agttttccct tgacgatcca tacccccttt ggtagctttt
      421 ttgtagcact ctaaaattct gccgtgtcag gaggctcaat tcatgaccgg aagagaaatc
      481 gcgcagatgg ccttcgattt aaagaagcct ccccgagtgc ccgttacctt gatcggaggg
      541 ggcgcctggg cagttcatca tgcaggaaag accttcgggc agatcaaaaa tgacccgaag
      601 cagatcgccg atgtcttcat ccggtttttt cgcctcttcc agaacgacct gatctggacc
      661 gggtcgaact tcctgaacta ccccgttcat ttcttgggat gccccattaa ggatgactcc
      721 gccgacagtc cggggctgga aggcacggtc atccagagcc tggaggagat cgaccgcttg
      781 aagatcgaaa gagtcttcca gaacccaacc atgcaggcca tcatccgctc tcaccacctg
      841 attgccgatg ccctcggcag ggaaaccatg atcgtaccca cccagtgggc ccctttcacg
      901 cttaccgcgc ggatcctggg aatggagcct ctgctgatcg ccaccatcca ggaaccggaa
      961 aagctctccc ggctcatccg gttttccacc gagttgacct gggcccttct ggagcccatc
     1021 ttggagcacc cggatattcc cggggccaat ttttccgatc ccgtcgcttc cggggacgtg
     1081 gtttccccat ccaccttccg caaattctcc gccccggcca taaaggaact ggtaaaccga
     1141 gtcaaagcga aaaagaaata cagcatggtt cacatctgcg gcaactccac ccaggtcctc
     1201 aaagacgtcc tggagatcaa tccccattgc tttagcttgg agaataaggt ggacctcaaa
     1261 gcagccagag agattctggg aggcaaagtc tgcgtggccg gaaatgtatc ccccaccggg
     1321 gccttccttt ccggaagacc tgagcaggtc caagaagagg ccaaagcctg cctggctgcc
     1381 tggggagatg gggggggata cattctgacg gtgggctgcg atttctccaa gaacgtcccc
     1441 atggaaaaca tgaaggccct gatgtctttc cgaaaataag cccggcccgc aattcttttc
     1501 accggccgcg ggaatcgatt gaacgacgga atatggaacc gacttatccg aaaagccctc
     1561 agagtctttg ggcgggcgga ttgcttcttc tctttgtcct ggcccacttc tgccatcact
     1621 tgttggtggc cctgaccata cctttgtccc ccatgatccg aagcgatttt tccctcgatt
     1681 acacctacac cggtttggtg atttccgcct tcaacttggc ctacgggatc gggcagttgc
     1741 ccggcggatg gatggccgac cgcttcggcc cccgacggat gatcacgctt ggaatctggg
     1801 gggttgcctt ggccggtttt attgtgggcc tgtcccaaaa tttctggatg atggtcgtct
     1861 tcctggccct gatgggtatt ttgggcggtg gctatcatcc ggcggccgcc ccgatgatct
     1921 catcgctcgt agaaccccca cgccggggac gggccctggg actgcacatg atcggcggag
     1981 gggccagctt ctttctctcc cccctcatcg ccatggccat tgcggcctac tggagctgga
     2041 gggggtcctt catcgggctt gccgttccca ccctggcctt tggaatttac ttccacctgc
     2101 tcctgaagaa acgcgaggcc ggggagccgc tgggccaggt tcgcattcca cgcccctccg
     2161 agaaaattcc taaacagcgc ctgcttggcc ccctgatcgt ggtcatcttc ctcagcacct
     2221 tcagcgcggc cgtcctcttt tcggtcattt ccttcattcc tctgttcctg gtcgacaaaa
     2281 tagggtacag taaggagacg gccggaactt tctatgccct gatctattcc gctggactct
     2341 gggcgagccc catgggagga tacctttctg accggttcgg ccccattccg gttctcttat
     2401 ttgccctctt tctgaccggc ccggttatct ttttgctcca cttcgtttcc acttggctgg
     2461 gaatcggaat tctgcttcta tctctgggaa tgatcatcta cgtgcgcatg ccggtttcgg
     2521 aatcctatat tctctccaac accccggagg ggaggcgttc cgctgtcttg ggggtctact
     2581 tcttcgctgg catggaaggg ggcgggattc tcgctccggt catgggttac atgatcgacc
     2641 atctgggctt ttttgcaagc tatacatttg ctgcatcgat tatcattctg accaccttgg
     2701 tctgcgggat tcttttgcgg gggaatctaa aatggaaacc gtgatcgctt gggcgaatgc
     2761 tcatagttat agattttctt tcttgccttg cgtagatatt gtggtatatg atttagaaca
     2821 tacaacatct tgtgtgaatg accttataag acttagatat ttaacaaatc tcacccagcc
     2881 ttttgaataa ggagtgcccg ccgagatggg gaacgattgc ctcgccgagc tgaatccctt
     2941 cctttcgaaa aacgccctta ccgtcctgga aaagcggtac ctaatccgcg acaatggcaa
     3001 ggttctggag aacccgacgg acatgtttcg gcgtgtcgcc gaggccatcg cctctcccga
     3061 caaaaagttc gactctcagg cggacttggg aaaaatcgcc ggtgaatttt gccgcctcat
     3121 ggccggcctg gaatttcttc ccaactcccc cacgctcatg aacgcggggc gggaactggg
     3181 acagctttcc gcttgcttcg ttctccccgt cggggattcc atggaggaga tcttcgatgc
     3241 ggtcaaatac accgccctga tccacaaatc cgggggggga acgggttttt ccttctcccg
     3301 ggtccgcccg gccaacgacg tggtccgaac caccagcggc gtctccagcg gccccatctc
     3361 cttcatgaag gtcttcgaca tggccacgga aacgattaag caggggggga cccggcgcgg
     3421 cgccaacatg ggaatcctcc gggtggatca ccccgacatt ctgaatttta tccaatgcaa
     3481 gaccgaccag aaccatctca ccaactttaa catctccgtg ggcctcaccg aagctttcat
     3541 gagggccgtg gagaggggga cgccctactc gctgatcaac ccccggaacg gggaagcagt
     3601 ggggtcactg gccgctcgtg aggtcttcga ccggatcgtg aagcaggcct ggcagaacgg
     3661 agagccgggg atcgtgtttc tggaccggct caaccgggac aaccccacac cccatgtggg
     3721 gatcatcgaa tccaccaatc cctgtggaga acagccgctt cttccctacg aatcctgcaa
     3781 cctggggtcc atcaacctgg ggctgatggc caaggagggc cggatcgact gggaccgttt
     3841 gaaagaggtg gttcacctgg ccgtccactt cctggacaat gtgatcgagc ttaataagta
     3901 ccccttgccc cagatcgccg agatgaccca tgccaaccgt aagatcggcc tgggggtaat
     3961 ggggtgggcg gatatgctga tcgctctggg gattccttat aactccgagg aagcgatcgc
     4021 cctggccgaa aaggtcatgc aattcctcca ggcggaaggt catgaggcct cccgccgatt
     4081 ggccaaagaa agaggtgctt ttcccaactt caagggttcc atctatgacc aacgcgggga
     4141 gcctccgctt cgaaacgcta ccgtgactac catagctccc accggcacgc tctccatcat
     4201 tgccaacgcc tcctcgggca tcgaaccgat cttcgccgtg tcttttgtcc gacaagtcct
     4261 ggataacaac accctggtcg aggtcaatcc tcagtttgaa agaatcgccc gggagcaggg
     4321 gttcttctcg cccaaactca tgaaagagat cgccgagcat gggtccattc aagacctgga
     4381 agaaatcccc gccgaggttc gcaggctctt tgtgaccgcc cacgatatca ccccggaaga
     4441 tcacatccgt atgcaggcgg ccttccagaa atttaccgat aacgccgtga gcaagacggt
     4501 gaatttctcc cgccacgcca cggtggagga ggtgcgagag gttttcgagc tggcctatcg
     4561 gctgggctgc aaaggggtga ccatttaccg agacggttcg agggaccagc aggttctctc
     4621 caccggcagg aaagccgagg agacggaggg ggcggcggac aacgggaaga aggtagcccg
     4681 gaaacgcccc atggccctca aaggctggac ctatcgaatg cagaccggct gcggacccct
     4741 gtatgtaacc atcaacgaag acgaaaacgg gctctttgaa ctctttacca ccatggggaa
     4801 agcgggaggc tgtgccgctt cccagagcga ggccatcggg cgaatggttt ccctggcctg
     4861 gcggagcgga gtccatgtca actcggtggt caagcagctg atggatattt cctgccactc
     4921 tcagtccggg tttggggaaa accgggtccg ctcttgcgcc gacgcggtgg ccaaggccat
     4981 ccagcagcac ctgtcctcca acggccctgg agaaatcgag agggaagagg tcgaaaaagc
     5041 tccccttttc aaaggggcct gcccggagtg cgggggaatc gtggaacacg agggaggatg
     5101 ctcggtctgt catgcctgcg ggtacagcga gtgcgcttaa aactggttcc tggttcctgg
     5161 ttgctaattg caggttgtga aatgatcatt ggccgttgtt cattgaccat tgcccattga
     5221 taattgtcca ttggccatta tttattgacc attgatcatt tctggagagg atttttcatg
     5281 aagacctgtg cttactgttc ggagctgctc gacgatgggg taatggaatg cccgaagtgc
     5341 gggcgctcga tgttcattca ggcccggacc aaagctcctt ccgcgggctt ttccctcaaa
     5401 gaagacctat ccctgccgaa aagaaccctc agcgacctgc acctgggtgt cctggagttg
     5461 ctgtcggccc agccggactt cgtcaatgcc tcccagttca tcatgtccct ccccctagag
     5521 aatgaatccc aggcccagac agcggtgatg gccattgaag aaatggtggg agcgggattg
     5581 attgaggtga aggggcgcag caaaatccga atcagcccca aaggcaagga actgtatgat
     5641 cgagaaaagg acctgcccgg ccccgaacaa aaaaccgagt gggacgcaga taaacgcaga
     5701 tgaagacaga ttatcaatag gtaaaaaaac acgacttaag aacccgacta aggggactca
     5761 ggcaaacacg gatcgtagca ttcatatttc tgtgctctct gcgtaatctg cgaaatctgc
     5821 ggataaaaaa agggcggctg aattcatgtt gaaagaattc ggccgccctt tttgtctccg
     5881 gaatgacttt ctcttagcgg tctccctcct gggtgatccc gcccaatatc caggatttct
     5941 ctcctacgtt ccgggtaaaa atccagtact cgacgaactt caccgggtcg gaggaacttc
     6001 cggccacaat ttggccggtc ttttcctgga tggtgtaatc cagcaggttg gcgtaaaatc
     6061 tcacggtgac atattcttcg ccgcggtttt ggccggcttc tattatttcc acttcccgca
     6121 cggcgatgtt ttccagacga ttgatttcgt ggtcggcgat aagccggtct acctgttcct
     6181 tgaaaacttc gtacatggca ggagtgagga gggagcggat tcccttcaaa tctcgcctcg
     6241 tccaggctcc ctgaattttg aagaacatat cctcggccag gtccttgaag gccttctcgt
     6301 tgaaggtcgg gtccatggct ttgatgtggc gcatcgcatc gggggatggg ccctctttat
     6361 aatcctcttc ctccggaggc gggccgagaa tgggccccag agagggtccc ggcccggggt
     6421 agggatacgg agaaccgccc gccccggcca tattcatctg gagagtctgc cggtaacgga
     6481 agcgtttcac cacgaagtag atgatcccca ggataaccag aatcaggaaa atgtcggcca
     6541 ggccgaactc tccgccgaag ccggggccgg caaagcccag gctgctgaag agcatccccc
     6601 ccaggagccc gccgatcatg ccgcccatca aaccggagaa taagccacgg gtgggagagg
     6661 cgggaggaac cgcgggatcc ggttgtgtct gctgactctg cttctgctga taaggagaag
     6721 gttgagcggg ggttgtggaa cgcgaggtgg acccacccga gctgtagctg cgggtcccgg
     6781 aagaggttcc tccccccgcg cgggcgaaca cttcggattc cagacccagc caaacaagga
     6841 aaacgaatga aaaagccgct aatagaatcg cgcggcgatg ctgccagaat ctcttcatac
     6901 ctgaacctcc gtacttttga tctccgcgac tttgattatt attgcctgtt aatccaagga
     6961 ttgcaacttc caaatcgcat ttgatttgac tatccttgga acaaccccct ataatcgggt
     7021 gtatgaaata ttcctgttct tcctgccaaa ggctttttgc aatcagtgaa aaacgattcc
     7081 ggtgcacctg cggcggcttc ctccgggtag aatattctgg actcttctcc aagaaggacc
     7141 tcgaacggcg cgaccattcc atctggaggt accgcgaggg gttcgggcta ccggaagagg
     7201 tccaacccgt atccttgggc gagggcagaa cgccgattat tggcagaacc tacgaggggg
     7261 ctgacctgac tttcaagctc gagtttcttc aaccctcggg gtcctttaag gaccggggag
     7321 ccagcgtcct cctctccctg gctaaaagcc tggggatttc ccacgtggtg gaggactcct
     7381 cggggaatgc cggggccgcc atttccgcct atgccgcagc cgcaggaatc cggtgcacgg
     7441 tctatgttcc ggattacacc cccgaaggaa agttaatcca gatgaagcta tacggctcgg
     7501 aggtggtcaa ggtgcccgga aaacggcagg aagccaaccg ggcggcgatc gaggcatcgg
     7561 agaaatctta ttatgccagc catctctgga atccctattt catcctaggg cttcaatccg
     7621 cggcttttga aatctgggag caatatgaag gggctttgcc gccccgggtg atcgtcccgg
     7681 tcggcggagg gggcttcttg gaagggctct attcgggatt tgccgccctc cgaaacgcgg
     7741 gctatgtgaa agaggtccca cgcctcatcg gggtccaggc ggagaattgc ccgccccttc
     7801 accgcgcttt ccacgagggt ctgcccgact accgggagat ggaggtggac acgaccgtgg
     7861 ccgaggggat tgcggtccaa aaacccccga gggggaaagc cgtactccag gccctgcggg
     7921 aaagcggggg gtggacggtg ggggtgaccg aagaggagat tctctccgcc gaaaaggccc
     7981 ttttctccat gggcctcttt gtcgaaccca cctcggccgc agccttggct gcctggcgga
     8041 agcttcccct gacggaaaag gatgggagtc tgatcgtcct caccggaaac gggttgaaag
     8101 aaacgcagaa gctttcgaac ttattcctgg ggccttccta agggcttcaa acgatttttc
     8161 tctgggtctg tccgggaaaa gacccggaag acatcccgcc gcgaattccc ggccatggtc
     8221 cggaatcttt tgtcccccag gcgctggttt cctttccccc cttcctcaag gacgcttccc
     8281 gcaggaacgg gagaggcaag aaaggattgg aaaaaatgac tcaccgggaa agaattctca
     8341 aggccatccg gggggagatg ccggaccgcc ttccctttgc cccccgtctg gacctctggt
     8401 ataacgccaa cgtgcaggcc gggacgctgc cggaaaggca taaaagccgt actttcgacc
     8461 aaatcgcccg ggccgagggc tgggccctcc acaagatcaa cccggaatac caaaaatccc
     8521 gcaaggccga ggacaatctc cactgggctc tgggaattct ttcttacaag gaatccgtct
     8581 tcggcttccg cttctccccg gacgtggacg tggaagcgaa agaaagcggc ggacggacaa
     8641 agatcatcta ccataccccg atcgggtcag tgagcacgac tacggtttac accgaagaga
     8701 tgaggaaagc cggggtctcc aatttctggg tggaggaaca tgtcctcaaa ggacccaagg
     8761 actatcgagt ggtggcctac atttttgaaa acctgaagtt gttccgcgat gaccgggact
     8821 tcctgaaatg gaaagaggaa atcggcgagg atggcgtgcc ttttaccctc gcagggcgcg
     8881 cggcatcgcc catgcatcat atccagaagt attttgtcga tgccaccgat ttctattttc
     8941 actatcacga ttatgccaaa gagacgcagg cgctcgcgga aagcatgggc ggcttcttcg
     9001 acgagattat tcaacaggtg ggcgattccc aggcagaagt ggtgtactgg ggcgcgaact
     9061 tcgatgacat gatcacctac cccgccttct tcgaaaagga cattgtaccc tggattcgga
     9121 aagccgcgga tatcctggac cggaagggca agtatgtctc ctgccattgc gacggggaaa
     9181 acctggggct catggacctg atccgcgatt cgggaatgca cattgccgaa gccatcaccc
     9241 cagcccccat gaccaaagtc cccatcgaag aatactaccg gcggtggagc gataaattga
     9301 ccctcttcgg gggaatcccc tcgatcctcc tcctgcgcga gtccatttcc gatgaagaat
     9361 tcgaagccta cctggatcac ctcttcaagg tcatcgcccc aggaaggagg atgatcctgg
     9421 ggatcgctga ctcaacccct cccaaagccg ttttcgagag gctggtgcgc atcggagaaa
     9481 aagtggagaa ggaaggccgc ctccccctcg aagcgggagg gttccgtcca ctgattcagc
     9541 cggtcgtgcc agaggcggcc aaaccggtgt tgcgggaaat cgtctgggac gaagagtgcc
     9601 gccaggtgca gaacgacgtc ctggaggggg accaggccgc cattcaggag catatccaaa
     9661 gcttgctggc caagggagtt aaagcggaag agatcctgaa acggggcatg ctttcggcca
     9721 tggaggtcat cagcgagcgg ttcaaaagcg gcgaggcgtt tattcccgag gtgcttcttt
     9781 ccgcccgggc catgaatgag gcggtcaagg tcttgcagcc gcacctcggc gggacgcagc
     9841 tggcagggaa gggcaaagtc ctgatcggaa ccgtgaaggg agacctccac gacatcggca
     9901 agaacttggt gatcaccatg ctgaaaggcg tggggttcga aactgtcgac ctgggaatcg
     9961 atgtcccaac ccccgccttc atcaaggcgg tggaggaaca taagccccac attctggggc
    10021 tatccgccct tctgaccacc accatgcccc agatgcaagc agtcatcgaa ggcctgaagc
    10081 agaagggtct ccgcgatcgg gtcaaggtca tggtgggcgg tgccccggtc aacgaaaaat
    10141 tcgcgcggga catcggggcc gacagctatg cggcggacgg ggcggatgcg gtaactctgg
    10201 ccaagaaact catacaacaa gagtaggggc agactttgcc tataacatct caaccggggg
    10261 ctgcttgatg aattgctcat tgctggtaga agcggcgtag cccttttcaa tgaatgattt
    10321 caatatcgaa actttttcga ttatcgggaa acagatggat agaaagcaga cattctcacc
    10381 ctacccgaaa cgggccgttg gcgtacggct gtattgaccg acctgaccag gcccccgatg
    10441 ctcagattag ttccgcagtt gcaaatgaac gtctcgctgg agtttgtcca aatataaagg
    10501 ccgttaagcg gtgctgcgcc tgtgccgtgg cgttccaaag aagtggagca ggagatactg
    10561 ggtaagcatc tcgacgaaaa gaccattccc cggggaggtg aagccgcatg aagaacgcag
    10621 agcctctcac gaaaaatgga tacaaggtcc ctctgttcag ggcggtcatc gaggaagaac
    10681 tcgcgagcat ggcgcgggga taaggccaat ctgaagcgga tcaatgctac accaactgat
    10741 aattttttga atattagcag tatcgagaca aacgacttct cggactttga aatggcccct
    10801 atcgaaagga gaaaaggcat gggaaagcaa atttctcggc gcagttttct gaagaaaggt
    10861 gccatcgcgc tggggactgc cggcgtaggc gattttaaag gtataggcag cgctgagcag
    10921 gtgagtagtt ctaagccttt gttaggtgaa gaatcgaaag agacaataga tcctcatgtt
    10981 atcgttaagc tctatcccgg aagatcagaa cagcagaaga ttcgacttgc cgaacaaata
    11041 gtgaaagacg ttgtctccat tataaagtgc ggagaagaat cggtctccgt agctattgaa
    11101 gaaatcaagg cagaggattg ggcagagaag gtctataagc cggatattct taacaccccg
    11161 gggaagctat acaagaaacc gggatactcc atgtgaacct ttcgactcct agcctgaagg
    11221 ccggggcctg ccttcgaccc aagctcaggc cgagcggctc aaggttcgcc ctgagccgcg
    11281 ccttcttccc caccttcaaa ggtggggctt cgggcggtcg aacgggtgaa taaccgggaa
    11341 tcgaaaaccg aaattgcaaa ttttctcaaa aaaacaatcg gaaggagaga aaaatgaaat
    11401 acattgcatt gcttttgtcg ctgtcacttt tggtttcaat gggttttttt gcgggccagg
    11461 cgctgaccgg tgaagccaag aaggagtcgc aaacgatatc acgggccggc tctcagcctt
    11521 ccgtcaaagg gtcgggcgaa tattttaccg gcaatgtccg tattgacccc ttgttcccgg
    11581 caaaagattc ggcgcctttt tccggcgcat atgtcacctt tgagcctggg gccaggtccg
    11641 cttggcacat tcatccgacg gggcagcacc tgcttgttac agccggggtt ggtcgaaccg
    11701 gggaatggga aggcaaggtc gaggagatca aagcgggaga cgtgatctgg tgcccgccaa
    11761 aggtaaaaca ttggcacgga gcttcaccca ccaccgcaat gacgcatatc gccatcacgg
    11821 gaacagtcgg cggcaaaaac gttgaatgga tggaaaaggt cactgacgag caatacaacg
    11881 ggaaataagg aggtcatcat ggataaaaaa agcacatggt tcctggcagg catttttatc
    11941 ctgattttgg gttgcgcaac taattcggag gcacagacgg tgaataccca aatgttaagt
    12001 cccaaacaaa agagcatcgt taccatttcg gcttttaccg ccaatggtga ccaggaaaag
    12061 ctggcaacag ccctcaatga aggcctggac gccgggctga cggtcaacga gatcaaggaa
    12121 attctcgtgc agatgtacgc ctattgtggt tttccgcgca gtttaaacgg aatcagtacc
    12181 ttcatggttg tcatggacga acggcaaaag aaaggcatca aggacgagat gggcaacgaa
    12241 gttagccccc tgccggccga catgaacaag gatgagtacg gggctagggt tcgggctaag
    12301 ctgagcggca gcgatgtcgt tccgccccca agcgggtacc agctttttgt tcccatcatc
    12361 gacaaattcc tgaaagagca tctttttgca gacatcttcg cccgcgacac cctggattac
    12421 cagagtaggg aacttgcgac catttctgcc ctagccagta tgacaggcac ggccggccag
    12481 cttcgtttcc atcttggcgc cgcgatgaac acggggttta ccgagacaca gatgaaggac
    12541 ttcatttccg tgctcaacgc caaggtaggc caaaaagaag ccgaaagtgc caaccaggtt
    12601 ctgacagccg tgttgcagag taggaaatag actcctattg caagaaccgg ataacgcctg
    12661 tgaaatatta aatagggttt ttagtaacag ggcaaaataa gaccctgtgc tgaaataggc
    12721 ataattaata gtcgtgggga tgaagtcgac ttcaccgccg acccttcaaa agcctggtgg
    12781 agggagacat gaacgctaag cagtttctaa ttcttgtcgc atcggtgctg acgttatctc
    12841 tatgcggggg tgcccttgcc gaggagatgc aaggtcgata cgtgcgccta gcagaactcg
    12901 aaatcgatcc cgctcagctg gaaagctata aaggtgtggc taaggagcaa attgaaactt
    12961 ctgttcgcgt cgagccagga gttctggctt tatatgccgt gtcgcaaaaa gataatcccg
    13021 cccacgtaat agtttttgaa atgtatactg atgcggatgc gtacagggcg catttggaaa
    13081 cgcctcattt caagaaatat aaggccaaga cacaggatat ggttaagtcc ctcaaacttg
    13141 tcgagactgt cccaattatt ctgagtgcga agccgaagtg aagacgatcg gcgcgggtgc
    13201 tcgggttagt ttgcttcttt ccagagtccg atccattcgc cgattccggc tttggccttg
    13261 cggcccgcgc cagcgcttct tctgagagga aaacatgatc gaacgaaggg aattcttgaa
    13321 gaaaatggcc ctgctggctg gggtcatggg cggaggcgcc cttcttcccg ccagcggaaa
    13381 gaccgccgaa caggaaattg gaatcgccgt tgttgaagga gaaaatccta aggctcaagt
    13441 tcaagaggcc atccgccttc tgggaggaat tcagcggttt gtccgccggg gagaccgggt
    13501 tgtccttctt ccgaaccccc agggaaccgg gcggggggtc accaccaacc cggatatggt
    13561 ggctgaaacc gtacggcttt gtctggcggc cggcgtctct tcggtgagcg tatcttcctg
    13621 ccacggccgg gaagactggc tcgggacggg catcatcgag aaagtcgaag cttcaggggg
    13681 gcggatgaaa tatcctgatt cggaccgtga ctgggcaacc atcagcattc ccaaggcccg
    13741 ggtccgaaaa gaagtgaagg tgatccggga tgccgtacag gccgatgttc tcatcaacat
    13801 gcccatcttc aaacagcata actacacccg ggtcacgggc tgcctcaaga atctgatggg
    13861 gacgaatgac gacaactggt ccttccataa gggagatctc cacttgcacc aagccattgt
    13921 ggacctggct tcgatctttt cccccgccct ttgcctggtc gatgccacga ccattctcac
    13981 gcaaggaggg ccctttggcc ccggaagagt gatccatccg aaaaaggtgt acgctggcaa
    14041 agatatggtc gggctggacg ccctctgttg tgatttgctg aacgt
//
LOCUS       DASPRR010000590         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2240739, whole genome shotgun sequence.
ACCESSION   DASPRR010000590 DASPRR010000000
VERSION     DASPRR010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240739"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VEL68_21695"
     CDS             <1..1025
                     /locus_tag="VEL68_21695"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38659.1"
                     /translation="NALGGATVAGKKYKVQIVSMDDQFQTALTVNNAKRLVHQEGARM
                     VYNPSSGGIFGLMAINEKENFIVGAYTTNQKCITAGNKLIFRGPPSMLVYVRNWADRA
                     MAKGYKKCAMMPDAYDYGKLWSGLFETYWKEKGGTITTNIPVDFMKVTDFYPLLTKAL
                     ATQPDVILIGSSSEPDAMQIQQARELGFKGGFIIIERGKLEEMERIVTKMDALNGCIG
                     VAPFLMYPGENIKKLADKFYKKYGKDKIITHETGIAYANGLGYAGSVMVAGSTTDPQK
                     IMKAMLSGEQFKIPFMKDNDPYYTDYIYPNGAIKGKIFGVEMADGKYGAPFPVVSPDW
                     VYTKEY"
     gene            1124..1984
                     /locus_tag="VEL68_21700"
     CDS             1124..1984
                     /locus_tag="VEL68_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38660.1"
                     /translation="MGIFLQTIINGIMLGSIYALVALGLTLIYGILEIPNFAHGALYM
                     VGAYISFLVITSLGIPYWIALVLSAAFLFFVGMFVERVVYRPLYAQPVINSFIAAVGL
                     ILILENGALALWGPQFRRFPRATAQVFQVLGVTITSQRILVIVLTVLLILATHLFIKR
                     TRWGAAIEATSQNRDGAQLMGINVSLVGGITFGIGTALAAVAASLIAPILLIYPTMGG
                     PVIGMAFVIIILGGMGSFFGAVVGGYLIGLAETLFSSYVTTNYVEALIFGILVFVLAV
                     KPTGLFGKAH"
     gene            2066..>2378
                     /locus_tag="VEL68_21705"
     CDS             2066..>2378
                     /locus_tag="VEL68_21705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38661.1"
                     /translation="MAKKFYYLLLTALLILAPLVITGQYPQHIMVMCGIWVIASLALN
                     LLLGYTGQLNLAHGTFFGIGAYATALLVQKLGFSFWLALPLGCAITAFMGFLTGLPTL
                     RT"
BASE COUNT          498 a          690 c          660 g          530 t
ORIGIN      
        1 tcaacgccct gggcggggcc acggtggccg ggaagaagta caaggtccag atcgtcagca
       61 tggatgacca attccagacc gctctcacgg ttaataacgc caaacgcttg gtgcatcaag
      121 agggggcccg gatggtctac aacccttcca gcggggggat ctttggattg atggccatca
      181 acgagaagga aaatttcatc gtcggcgctt ataccaccaa ccagaaatgc atcaccgccg
      241 ggaataaact catcttccgg ggaccgcctt ccatgctcgt ctatgtgcgc aactgggcgg
      301 accgggccat ggccaagggg tataagaaat gcgccatgat gcccgacgcc tacgactacg
      361 ggaagctttg gagcggattg ttcgagacct actggaagga aaaaggcggc acgatcacca
      421 ccaacatccc cgtggacttc atgaaggtca ccgattttta tcctttgctg accaaggccc
      481 tggccaccca gccggatgtg atcctgatcg gctcatcctc ggagccggat gccatgcaga
      541 ttcaacaggc ccgggagctg gggttcaagg ggggcttcat catcatcgag aggggaaagc
      601 tggaagaaat ggagcggatc gtgaccaaga tggacgccct gaatggatgc atcggggtgg
      661 ctcccttcct catgtacccc ggggagaaca tcaagaagct ggcggataag ttctacaaga
      721 agtacgggaa ggataagatc atcacccatg aaaccggcat cgcctacgcc aacggcctag
      781 gctatgcggg gagcgtcatg gtcgccggga gcaccaccga cccgcagaag atcatgaagg
      841 ccatgctgag cggcgagcag ttcaaaattc ccttcatgaa ggacaacgac ccatactaca
      901 ccgactacat ctacccgaac ggggccatca agggaaagat cttcggggtg gaaatggcgg
      961 acggcaagta cggcgcgcct ttcccggtgg tgtctccgga ctgggtgtat acgaaagaat
     1021 attaataagg gatcggtgtc agtgtctagg gtcagtgtag aaggaaaaaa tttcttttct
     1081 tttactgacc ctaatcactg acactgacac cttgaggttt ttcttgggca tcttcctcca
     1141 gaccatcatc aacgggatca tgctgggctc tatctatgct ctggtagccc tggggttgac
     1201 cctgatctat ggcatcctcg agattcccaa tttcgcccac ggcgcccttt acatggtggg
     1261 cgcctacatc tcttttctgg tcatcacctc cctgggaatc ccttactgga tcgcgctggt
     1321 tctctcggcc gccttccttt tttttgtggg catgttcgtg gagagagtgg tctaccgccc
     1381 tctctacgcg cagccggtca tcaactcttt catcgccgcg gtgggactca tcctgatcct
     1441 ggagaatggg gctctggccc tctgggggcc ccagtttcgg aggtttcccc gggcgacggc
     1501 ccaggtcttc caggttctgg gcgtcaccat tacctcgcag cgcatcctgg tgatcgtcct
     1561 gacggtcctg ctcatcttgg cgacccatct cttcatcaag cggacccgct ggggggcggc
     1621 catcgaggcc acttcgcaga accgagatgg ggcccaactg atgggcatca acgtcagcct
     1681 ggtggggggg attaccttcg ggatcgggac ggcgctggcg gccgtggccg cgagcctgat
     1741 cgctccgatc ctcttgatct atcccaccat gggaggaccg gttatcggca tggctttcgt
     1801 catcatcatc ctcggcggaa tgggaagctt ctttggagcc gtggtgggcg gatacctcat
     1861 cggactggcg gagacccttt tctcttccta tgtcacgacg aattacgtgg aggcgctcat
     1921 cttcgggatt ctcgtcttcg tcctggcggt caaaccgacg ggattgttcg gaaaggcgca
     1981 ttaaaagact ccagtttcaa gtatcgggtc tcgagtttca acttatagac ccgaaacccg
     2041 agacttgaaa cccgaaactc atttcatggc taagaaattc tactacctcc tcctgaccgc
     2101 tcttttgata ctagctccac tagtaatcac cgggcagtac ccccagcaca tcatggtcat
     2161 gtgcggaatc tgggtcatcg cttccctggc tctcaacctc ctcctgggat acacggggca
     2221 actcaacctg gcccacggaa ctttcttcgg gatcggcgcc tacgccacgg cgctcttagt
     2281 gcaaaagttg ggtttttcct tctggctggc cctccccctc ggctgcgcga ttaccgcctt
     2341 catgggattc ctgaccgggc tgcccaccct ccggacaa
//
LOCUS       DASPRR010000591         6684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2081940, whole genome shotgun sequence.
ACCESSION   DASPRR010000591 DASPRR010000000
VERSION     DASPRR010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081940"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1043
                     /locus_tag="VEL68_21710"
     CDS             153..1043
                     /locus_tag="VEL68_21710"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HXZ38662.1"
                     /translation="MRLKEYLIATRPWSFTMSLISVSVGTLIAAEEGPILWGWYALVC
                     LGIVCFHATANVYNDFFDTRYQVDQPDSPTARYRPQPILTGMFTPRQLLIEGMILNAI
                     TIAIGMTLSFQRSFLVFWIGFIGFLASVFYTAGPVKYKYRALGELFVFLMWGPLMFEG
                     AYAVQLQALSMKVFYLSIPFGILVALVLLANNIRDIDYDSRQKIKTIGILLGGRQSFR
                     VYAVLILAAYLYIIFMVILGILSPWGLLVLLSLPKAIHLLKTFTQRIPEAADAITAQL
                     DTIFGLLLIAALILNRTVPL"
     gene            1040..1633
                     /locus_tag="VEL68_21715"
     CDS             1040..1633
                     /locus_tag="VEL68_21715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXZ38663.1"
                     /translation="MIGGAVLLAFICWAVTFGWAGGNFWVKIGLSVLVVFTYSLFFQK
                     PKVRWRMRSFSLGFLSAVVLYFLFYAGHLLAPYILPGAKTQVGGIYFLGEGTSKVLIF
                     LLLFFITGPGEEVFWRGFLQDHLMKKWGDFQGFVLATFIYAGVHVFSFNPMLILAALV
                     AGTFWGLLYLWKRDLLAQITSHSFWSAVIFAVAPIQG"
     gene            complement(1649..2896)
                     /locus_tag="VEL68_21720"
     CDS             complement(1649..2896)
                     /locus_tag="VEL68_21720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXZ38664.1"
                     /translation="MKWMDIANKLFKPRNEKLPVICIFGAGRLKLITPVQEDYEEKRL
                     DCRCYSADSSLEKVLVQDKPQVLISIGKRSTFPNLIKAPFEVRKRWIHYETLPDVNQL
                     GLDAYNCYLRNLFKVQGPEDDPLVTVFTPAYKTGEKIRRPFQSLREQTYTNWEWILVD
                     DSDDGGQTFRTLNDLARKDHRIQVFKPWEHSGMIGKLKNWAGSLGKGHILVELDHDDA
                     LTDCALDYVAKGFRQFPGAGFLYTDCAEVFEDGTNSTYRKGWAFGYGSYTDVEYKGKL
                     YKSGSGGNINAKTIRHIISCPNHIRAWRKCFYESIGGHNKELHVADDYEILIRTFLNT
                     RMVRVPKLGYIQYIGNSTQQVRNGDIHRHVRSLRAHYDKMIHDRLLELGCDDFAWDEK
                     KGCSDYTLPNPELESHANWIARV"
     gene            complement(3106..3300)
                     /locus_tag="VEL68_21725"
     CDS             complement(3106..3300)
                     /locus_tag="VEL68_21725"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXZ38665.1"
                     /translation="MFGIGSQELMIILLIAFLVFGSKRLPELGNSLGQAIRGFKKAME
                     GEPKKLESHETQDQESTTRK"
     gene            complement(3374..3679)
                     /locus_tag="VEL68_21730"
     CDS             complement(3374..3679)
                     /locus_tag="VEL68_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38666.1"
                     /translation="MRTKLQRIKFLLAIGISFLLAAYPAYLQYNSFIEIDFLSTHPSF
                     ENIDQENLLADEQNKVKIFVVNSSPEVLLFGIFLEEQVPPPSSQIFSPRHPASILRC"
     gene            complement(3823..4767)
                     /locus_tag="VEL68_21735"
     CDS             complement(3823..4767)
                     /locus_tag="VEL68_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXZ38667.1"
                     /translation="MKGSWVEGQNPSGNKTDSCEPGFLPPAAAAATGILVGSAMVATR
                     FVIDQTAPASLALFRYLIGFCCLLPPVILSGRVRLEGRDLLPIGLLGIAQFGILIALL
                     NYSLQFIPSARAALIFATMPLMTMAFAAVLGHERFTWSKTLGVLLTIAGVGFAVGEKA
                     IQRAGEAGDWTGELAVFGSTLSGAICSVLYRPYLRKYPTLPVSAFAMLASVGFLAVLA
                     AGEGFFNSLPRFTGIGWLAVLFIGVNSGIGYYLWLWALNHTSPTKVTVFLALSPITAA
                     ILGSLLLGENISMMSFLGLVFVALGLWIAHLSGSSPID"
     gene            complement(4764..5693)
                     /locus_tag="VEL68_21740"
     CDS             complement(4764..5693)
                     /locus_tag="VEL68_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HXZ38668.1"
                     /translation="MGDSDQYRRIEAVRRFNRFYTQQIGVLQERLLKSPFSLAEARLI
                     YELAHRGKTTATELSTQLGLDAGYLSRLLQSLNGRGLIERQVCRTDSRQNLLSLKEKG
                     KKAFATIHVRSRNEIGKMLNPLSPPDQNRLVKAMGTIEELLGARPEEKAPYLLRPHQP
                     GDMGWVVHRHGVIYSEEYRWDEQFEALVAGIVSKFIQRYKPKKERCWVAEKDGEIVGS
                     VFLVRKSNRIAKLRLLLVEPKARGLGIGSRLVDECVRFARHAGYRKIALWTNHVLHAA
                     RHIYEKAGFRLIQEEPHHSFGHDLIGETWELVL"
     gene            complement(6062..6259)
                     /locus_tag="VEL68_21745"
     CDS             complement(6062..6259)
                     /locus_tag="VEL68_21745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38669.1"
                     /translation="MKQTSRWNIRQMKCPEGKGHAELLLEWKVERGRKTLHSISCNHP
                     QLADYGGLECQWCCLEKITKK"
     gene            complement(6471..>6684)
                     /locus_tag="VEL68_21750"
     CDS             complement(6471..>6684)
                     /locus_tag="VEL68_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HXZ38670.1"
                     /translation="SALSFVQLGARPPMPEWGLMISQARNFIVNYWWIPTFPGLAIAL
                     AVGGFMFLGDGLRDLWDPQLRGRGRF"
BASE COUNT         1538 a         1827 c         1675 g         1644 t
ORIGIN      
        1 gagacaccag ctaaggatca atttccgccg gtctttttcc ataaccaact ttctctcttt
       61 atgaggcctg aaatccgcat tctattctcc ttgccagcta tttccctttc ctctatactg
      121 aagccatgca tgaatggaag agaactttat ccatgaggct caaggaatac ctcatcgcta
      181 cccgcccctg gtctttcacc atgtcgctca tttccgtatc cgtggggacc ctgatcgccg
      241 cggaagaagg gccgatcctg tggggatggt atgcattggt ttgcctgggg atcgtctgct
      301 tccacgccac ggccaatgtg tacaacgatt ttttcgacac ccgctaccag gtggaccaac
      361 ccgattctcc caccgcccgc taccgtcccc agcccatcct gaccgggatg ttcaccccga
      421 ggcagcttct gatcgagggg atgattctca atgccattac catcgccatc ggcatgaccc
      481 tttccttcca gcgttccttc ctggtctttt ggatcggatt catcggtttt ctggccagcg
      541 tattctacac ggctgggccg gtcaaataca agtaccgcgc gctgggggaa ttgttcgtct
      601 ttctcatgtg ggggccgttg atgttcgagg gggcctacgc cgttcaactc caggctctct
      661 ccatgaaagt cttttacctt tcgattccct tcgggattct ggtagccctg gtcctactgg
      721 ccaataacat ccgggatatc gattacgatt ccagacagaa aattaagacc atcggcatcc
      781 ttctcggcgg gcggcagagt ttccgggtct acgccgtcct catcctggct gcttaccttt
      841 acattatctt catggtgatc ttggggatcc tgagcccctg ggggctgctg gtcctcttat
      901 ccctgcccaa agcgatccat ctactgaaaa ccttcacgca aaggatcccg gaggccgccg
      961 acgccatcac ggcccagctg gacacgatct tcggccttct gctgatcgcc gctctgatcc
     1021 tgaaccgaac ggtgcccctg tgatcggggg agcggtcctt ctggccttta tctgttgggc
     1081 ggttactttc gggtgggcag ggggaaactt ctgggtcaag atcggcctct ccgtcctagt
     1141 cgtctttacc tattccctat tctttcagaa accgaaggtc cgttggcgaa tgaggtcttt
     1201 ttccttgggg ttcctttccg ccgttgtcct gtattttctt ttttacgccg ggcaccttct
     1261 ggccccctac atcctccccg gcgcgaaaac tcaggtgggg gggatttact ttctgggaga
     1321 gggaacgagc aaggttctca tcttcctcct gctgttcttc atcaccggtc ctggcgagga
     1381 agtcttctgg cgaggatttt tgcaggatca tctgatgaaa aaatgggggg actttcaggg
     1441 gtttgtctta gcgaccttca tttacgcggg ggttcatgtc ttttccttca accccatgct
     1501 catcctggcc gccttggtgg ccgggacctt ctggggcctt ctctacctct ggaaaaggga
     1561 cctcctggcc caaatcactt cccactcctt ctggagcgcg gtcatcttcg ccgtggcgcc
     1621 gattcaggga taatcggccg aagttccgct agacccgggc gatccagttg gcatgggatt
     1681 caagctcagg attcggaagt gtataatccg agcagccttt cttttcatcc caggcaaagt
     1741 catcgcatcc gagttccaaa agccggtcat gaatcatctt gtcgtaatga gctcggagag
     1801 acctcacatg acgatggata tctccattcc ttacctgctg ggtgctgttg ccgatatact
     1861 gtatgtagcc tagcttggga acccgtacca tccgggtatt cagaaaggtc ctaatcaaaa
     1921 tctcatagtc gtcagccaca tgaagctcct tattatggcc gccgatggat tcataaaaac
     1981 atttcctcca ggcgcgtata tggttggggc aggatataat atgccggatc gtcttggcat
     2041 tgatattccc gccgctcccc gatttataca gcttgccctt gtattccaca tcggtatagg
     2101 aaccatagcc gaaggcccag cccttccggt aggtagaatt ggtcccatcc tcaaaaactt
     2161 cagcgcagtc tgtgtatagg aacccggcgc cggggaactg cctgaatcct tttgccacgt
     2221 aatccaaggc acagtcggtc aaggcatcat catggtccag ctccacgaga atgtggccct
     2281 tcccaaggct gccagcccag ttcttgagct ttccgatcat tccggagtgc tcccatggtt
     2341 taaagacctg tatccgatga tctttcctgg ccaggtcatt aagcgtccta aatgtctgcc
     2401 cgccgtcatc cgagtcatcc actaggatcc actcccaatt ggtataggtc tgctccctga
     2461 gggactggaa tggccggcgg attttttccc cggtcttgta ggcaggggta aaaacggtca
     2521 cgaggggatc atcttctggt ccctggacct taaataaatt tcgtaagtaa cagttatagg
     2581 catccaggcc aagttgattc acgtccggga gagtctcata atgaatccat ctctttcgga
     2641 cctcgaaagg agctttgatt aagttgggaa aggtggaccg ttttccaatc gatatgagga
     2701 cttgcggttt atcctggaca aggacttttt caagactgga atcagcggaa taacagcgac
     2761 agtcgagtct tttctcctcg taatcttcct gtacaggagt gattaatttt aagcgaccgg
     2821 ccccgaagat acaaatgacc ggtagcttct cgttccttgg cttgaataat ttattggcaa
     2881 tatccatcca tttcatgggg gtccaaagaa catacgatgg ataaccgatt tgagagcagg
     2941 ctggaatacc agcagcggcc tcatgggatt aatcagctag ccccataagg attttattgg
     3001 taaaataata tgcctttcca agttggggcg cgccaaaacc ggtccttact gggtgccctg
     3061 ataccctagt aggggtattc cgcccaggcg gaatgcccct acggatcatt tcctcgtcgt
     3121 gctttcttga tcttgagtct catgggattc caacttcttg ggttctccct ccatcgcttt
     3181 cttgaatccc cgaatcgcct ggcccaagct gttccccaac tccggaagac gttttgatcc
     3241 aaagaccaaa aaggcgatta agagaatgat cataagttct tgtgaaccaa taccaaacat
     3301 aaaaattacc tcctgatttg aatgattagc ccaagggtaa aacggtttaa ttacgagagg
     3361 aaaaattgac ttctcaacaa cggagaatgg atgctgggtg acggggagaa aaaatttggg
     3421 aagaaggggg cgggacctgc tcctctaaga aaatcccgaa aagaagaact tccggggagg
     3481 aattcaccac aaatatcttt accttgttct gttcatcggc caggagattt tcctggtcaa
     3541 tattctcgaa agacggatgg gtggataaaa aatcaatttc gataaaacta ttatactgaa
     3601 gataagccgg gtaagcggca aggagaaagg aaatcccgat cgccagcagg aatttgattc
     3661 tttgaagctt cgtcctcata ctagaattcg ctctttaaat aaaaccttcc tataataatt
     3721 ctttgctgaa atttctgagc tgtcaaggtc ttttttcggg ggcagtcgaa aactttccat
     3781 aaaggcgata agcgtaaatg aatcagggac ccaatccccc catcaatcga tcggcgatga
     3841 tcctgaaagg tgggcgatcc acaggcccag ggcaacaaat accagtccca ggaaggacat
     3901 catggaaatg ttctctccca gcagcagaga ccccaaaatc gctgcggtga ttgggctcaa
     3961 cgccaggaac acggtgactt tcgtcggaga tgtgtggttc aaagcccata gccagagata
     4021 gtacccgatg ccgctgttaa ctccgatgaa aaggacggcc agccaaccga ttccggtaaa
     4081 gcgcggcagg gagttgaaaa aaccctcccc ggcggcaaga actgcgagga accctaccga
     4141 ggcgagcatg gcaaaggcac tcaccggcag ggtcggatat ttccgcaggt aaggccggta
     4201 taaaacgctg caaatggccc cactcagggt gcttccgaaa acggccagtt ccccggtcca
     4261 atcgccggcc tcaccggccc gctggatcgc cttctcccca accgcaaacc caacccccgc
     4321 gatggtcagt aagacaccaa gggtttttga ccaggtgaat ctttcatggc cgagaaccgc
     4381 agcgaaggcc atggtcatca aaggcatggt ggcaaagatc agtgccgctc gggccgaggg
     4441 aataaattgt aaggagtaat tcaggagcgc gatcaggatg ccgaactggg caattccgag
     4501 aagcccaatg ggcaataggt cccgcccttc caacctcacc cgcccggaca aaatgaccgg
     4561 gggcagcagg cagcagaatc cgatgaggta gcgaaaaagg gccagcgagg ccggggcagt
     4621 ttgatcgatg acaaaccttg tggccaccat ggctgagccg accaggatgc cggtcgccgc
     4681 tgccgcagca ggagggagga acccgggttc gcaagaatcg gttttgttcc cggaagggtt
     4741 ctggccttcc acccatgacc ctttcacaaa accaactccc aggtttcccc aatcaggtca
     4801 tggccgaaac tgtgatgggg ttcttcctgg atcaaccgaa atccggcctt ttcatagata
     4861 tgccgggccg catgcaaaac atggttcgtc cacagggcga tcttccggta acctgcgtgg
     4921 cgggcgaagc ggacacactc atccactagg cggctgccaa ttcccagccc gcgggccttt
     4981 ggctcgacca gtagcaggcg cagtttggcg atccgatttg atttcctcac cagaaacacg
     5041 gaccccacga tctccccgtc tttttctgcc acccagcagc gctccttttt cggcttatat
     5101 ctttggatga atttcgacac aatccctgcg actaacgcct cgaactgctc atcccagcgg
     5161 tattcttcag agtagatcac tccatggcga tggaccaccc accccatatc gccgggttgg
     5221 tgaggtcgga gaagataggg ggccttctcc tcaggtcggg cccccagcag ttcttcgatg
     5281 gtgcccattg ccttgaccaa gcgattttga tccggggggg ataatggatt caacatcttc
     5341 ccgatttcgt tgcgcgaacg cacgtggatc gtagcaaagg ccttctttcc cttctccttc
     5401 agggacaaaa ggttttgccg gctgtcggtc cggcagacct gccgctcgat caggccacgc
     5461 ccgttcaagc tctgcaagag gcggctcaga tatccggcat ccaatcctag ctgggtgctg
     5521 agttcagtcg cggttgtctt tccccgatgg gcaagttcat agattagacg cgcttcagcc
     5581 agagagaagg ggctcttcaa cagtctttcc tgtaataccc cgatctgttg agtataaaac
     5641 cgattgaagc gccgtacagc ttcaatgcgt cggtattgat ctgagtcgcc catcttcgct
     5701 ctccttgggg gaatttcttc atttgtttgc tattatcaac catttatttg cttaaatcaa
     5761 ccaattaatc gatggaagcg aaacccggaa ggttaactgt gcaagcgttc tttctattca
     5821 gatacctgtc ttttggatgt agtggatgac ctgagggaaa aaatattcaa gaaaaagaaa
     5881 ttcatgggag aatttcttca agataaatag aatttccagg tatggctcgc ggaagggtcg
     5941 ccttggggat cggcaaagcc atggaggata gcctgaacgg aacccggagg atgaaagccc
     6001 ggatgggagg cagtatctga ccgcctgtag aatcaacttc caatgatggc tatagggtac
     6061 cttatttctt tgtgatcttt tccaagcaac accactggca ttccagtccc ccgtagtcgg
     6121 ccagctgggg atggttgcag cttatgctgt gcaaggtctt tcttcccctt tccaccttcc
     6181 attcgagcaa caattccgca tgtcccttcc cttccgggca cttcatctgc cggatgttcc
     6241 atctcgatgt ctgtttcatg gctgaacctc ctcggataat aatagttttt ttacccctgg
     6301 aaggcaagct tcctccaagg ctaccaaagt tatcattctt ggcaagccaa agaccggcag
     6361 aaacaggaaa gagtgatgat caaggaaaga agcggggcca tgcctgactt ttccttcagc
     6421 cggctcatca gcatttccgt tcactatttc gctcgggtgc tggttgaaaa tcagaaccgt
     6481 ccgcgccctc tcaattgcgg gtcccagagg tcccgcaggc cgtctcccaa aaacataaat
     6541 cctcccacag ccaaggcaat ggccaacccg ggaaaggtgg gaatccacca gtagttgacg
     6601 atgaaatttc gcgcctggga gatcatcagc ccccattcgg gcatgggcgg ccgggccccc
     6661 agttgaacga aactgagggc cgag
//
LOCUS       DASPRR010000592         3811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_146574, whole genome shotgun sequence.
ACCESSION   DASPRR010000592 DASPRR010000000
VERSION     DASPRR010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146574"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..882
                     /locus_tag="VEL68_21755"
     CDS             295..882
                     /locus_tag="VEL68_21755"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HXZ38671.1"
                     /translation="MISEQETVSLQSKRKADWEVLFRVFALAIWCLAAGACAHPISAL
                     VRAEADPALTLSKVVENPKTYLGSIVIWGGIVSAVSRGPEGTRLLVIQTPLDSRGYPQ
                     TRVAEGEFIARIPESLDPRVYQRGLAITLAGEIEGVEEKDLGFMEYPRPVLRIVQVHP
                     WEGKHFPLTEGNWKGELYFPSSPSEDPFLEPGNEQ"
     gene            1078..2337
                     /locus_tag="VEL68_21760"
     CDS             1078..2337
                     /locus_tag="VEL68_21760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXZ38672.1"
                     /translation="MKIEQMTLSQKFIGLILLVFVLGIAIGLVIGCQYLIAFSPSDKI
                     PAEAEGSFRLIAEGWNTIQKRYVDREALKAQKITYGAISGMVNSLGDTGHSRFLTPEM
                     VKQETNQNRGRLEGIGAEVQMKNNRLVIVAPMDGSPAQKAGLNPGEIILKVNGEDVSD
                     LSLEEAVARILGPPGTQVTLTVLNPGTGLSRNVSIVRARISLHNVIWQLLPGTQVAQV
                     RISSFSNGITADLHKALEKIGEEGGTSLILDLRNNPGGLLDEAVGAVSQFLREGDVVL
                     EKDSVGRVTPVRVKPGGRAYDFPLVVLVNGGTASAAEIVAGALQDSGRAEIVGEKTFG
                     TGTVLEKFPLSDGSALLLAVQEWLTPKGKTIWHRGILPDRTIPLPAEVIPLVPRIGKK
                     ITSLELKESKDVQLQAALELLSKPGEK"
     gene            2567..3139
                     /locus_tag="VEL68_21765"
     CDS             2567..3139
                     /locus_tag="VEL68_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004725823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXZ38673.1"
                     /translation="MILENWINTYGYWAILLGTFLEGETILILGGLAAYQGYLKLPWV
                     ILIAFLGTVSGDQLSFYIGRKYGSKMLASRPAWQVRAKRVRRLLERYQDYLILLFRFL
                     YGLRILTPFVLGLSRVSRGRFLLLNTVGALVWAIIVGIGAFLFGQALEALIGDLKRFE
                     VEILGLVVLAGTVIWVIHGYRRRRSKASSH"
     gene            3277..>3811
                     /locus_tag="VEL68_21770"
     CDS             3277..>3811
                     /locus_tag="VEL68_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXZ38674.1"
                     /translation="MAPLETAFQGLTRGEARRRLEQDRANRLKASRRSTSLALFLGQF
                     KSPIILILIAAAILSFALRDAADALIILVIVFISGLLGFWQERGAHRAVEKLLALVQV
                     KATVLRDGAPEEIPLEEIVPGDVVALKAGDTIPGDCAILEARDLFVNEAALTGETFPV
                     EKTQGKHPAETPMTLRSN"
BASE COUNT          874 a         1002 c         1116 g          819 t
ORIGIN      
        1 gccgggaaaa tgatcgaagg gactaaagtg gccgagggtg aagtcgaaaa gggaatccag
       61 gacctgggca aagagattga gaagctcgga aagaaggtgg agaagctcgg ccgataaggt
      121 cagagattct tcatccaacc cccaaaagaa ggagacaacg atgatggaag gcaagattca
      181 atggacccgg attcgacccg gaaaagcaaa cccaagccgc cctggcaacc gttcaagcct
      241 cccgggcagc ggtagataaa gcctcggcaa ccttccaggc aacccggtgc gagaatgatt
      301 tccgagcaag aaacggtttc tttgcagtca aaacggaaag cggattggga ggtcttattc
      361 cgggtcttcg ctttggcgat ttggtgcttg gccgcagggg cttgcgccca cccgatctct
      421 gccctggtga gggccgaagc cgatccagcc ctcaccttgt ctaaagtggt tgagaatcct
      481 aagacctatc tgggttccat cgtcatctgg ggcgggatcg tctcagcggt ttcgcgaggg
      541 cctgaaggga caagactatt ggtgatccag actcccctgg actcgcgcgg gtatccccag
      601 acccgggtcg ccgaggggga attcattgcc agaattcccg aatccttgga cccgcgggtc
      661 taccaaaggg gcttggcaat caccctggca ggagaaattg aaggggtgga ggaaaaggat
      721 ctaggcttta tggaataccc ccgcccggtt cttcgaatcg tgcaggtcca cccgtgggaa
      781 ggtaagcact ttcctctgac cgaagggaat tggaaaggag aactctattt cccctcttcg
      841 ccctccgaag acccatttct cgaacctgga aatgaacaat aaacatgaag gaaccatgaa
      901 tcatgggact ggtcatggca gccaccgggg atcgaattca ttgccatcag gctaaggcac
      961 cttctccctg gattgtcctg aatggaggga acgaacaaaa tcggcaagag ctttccggat
     1021 attccgcacc tgctttgctg gaacacctct cgggatgatg acagggagcc caaaatcatg
     1081 aaaatagagc aaatgactct ctctcagaag ttcatcggtt tgattctctt ggttttcgta
     1141 ctgggaatcg cgatcggact ggtcatcggc tgtcagtatc tcattgcttt ttccccttcg
     1201 gacaaaatcc ccgcggaggc cgaaggatcc ttccggctga tcgccgaagg ctggaatacc
     1261 atccagaaac gctacgtgga ccgagaggcg ttgaaagcgc agaagataac ctacggagcc
     1321 ataagcggca tggttaattc tttgggagac accgggcact cccgcttcct gaccccggaa
     1381 atggtgaaac aggaaacgaa ccagaaccgt ggaaggttgg aaggaatcgg agcagaggtc
     1441 cagatgaaaa acaaccgcct ggtcatcgtg gctcccatgg acggctcccc ggcgcagaag
     1501 gctggattga accccgggga aatcatcctg aaggtgaatg gggaagatgt atcggacctc
     1561 tccttggagg aggccgtggc ccggattctg ggccccccgg ggacccaggt gacactgact
     1621 gttctcaacc caggaaccgg actcagccga aatgtttcca tcgtccgggc acgcatctct
     1681 ttacataatg tcatctggca gttgcttcca gggacccaag tggctcaggt gcgcatctcc
     1741 agcttcagta atggaattac ggcagacctc cataaggcct tggagaaaat cggggaagaa
     1801 ggggggactt ccctaatcct ggacctccgg aacaacccgg gaggccttct ggacgaggca
     1861 gtgggcgccg tgagccagtt tctcagagaa ggcgatgtcg tcttggaaaa ggacagcgtc
     1921 ggaagagtta cacctgtccg ggtcaaaccc ggaggtcggg cttacgattt tcccctggtg
     1981 gtcctggtga acggaggaac ggccagtgcc gccgaaattg tagccggggc gctgcaggat
     2041 tccgggaggg ccgaaatcgt cggggagaag actttcggta cgggaaccgt gcttgaaaaa
     2101 tttcccctct cggatggatc ggctttgctt ctcgctgtcc aagagtggct gaccccgaag
     2161 gggaaaacga tatggcatcg gggaattctt cccgacagga cgattcccct gccagctgaa
     2221 gtcatcccgc tggtcccgag aattgggaag aagatcacct ccctggagtt aaaagagagc
     2281 aaggacgttc agctgcaggc cgccctcgag ttgctatcca agccggggga gaaatagggg
     2341 tcgtcttctt catcttcaaa gcgggttgaa catagcgtag atgctctctg ctcctatctt
     2401 ttaattcgga cccacggacc ctgcgagggg gacagggcat ccgggaatga atcaaggggt
     2461 acctttcgtt ccaccatagt gcattccctt caatctatgc tcggattgat ttcgtcctag
     2521 aaaattctat ggaccttttg gccaactcct gctacctttt tggaaaatga tcctggaaaa
     2581 ttggataaat acgtacggct actgggccat actcctcggg accttcttag agggagagac
     2641 cattctcatt ctcggaggat tggccgccta ccaagggtat ctgaaattac cctgggtgat
     2701 tctgatcgcc ttcctgggaa cggtgagcgg ggaccagcta tccttttaca taggccgcaa
     2761 gtacgggtcc aagatgctgg ccagtcggcc cgcatggcag gttcgggcca agcgggtgag
     2821 aaggcttctg gaaagatacc aggactactt gattctcctt ttccggttcc tttatggcct
     2881 aaggattctg actccttttg tgctgggatt gagccgggtt tccagggggc gattcctcct
     2941 gctcaacacg gtcggagccc tagtttgggc gataatcgta ggaattgggg catttctttt
     3001 cgggcaggcc ttggaagcgc tgatcgggga cctcaaacgg tttgaagtcg aaattctggg
     3061 actggtagtc ctagccggga ctgtgatctg ggtgatccat ggatataggc ggagaaggtc
     3121 gaaggcttca tcccattaaa ccagggtccc acgctgtctt tctcgtggct cggccagaaa
     3181 tacgggaccc atcttcccgc gaaacggctt ttttccaagg attcggagat gcaggagatc
     3241 cccggagctt tttggagttt ttccggtcag gagctattgg ccccgcttga gacggccttc
     3301 caggggctca cccggggcga ggcccggcgg cggttggagc aggacagagc aaatcgtttg
     3361 aaggcctccc gccggtctac ctccttggcc cttttcctgg gtcaattcaa aagcccgatc
     3421 atcttgattc tcatcgccgc agctatccta tccttcgctc ttcgcgatgc cgccgatgct
     3481 ctaattatcc tggtgatcgt cttcataagc gggctgctcg ggttctggca ggagcggggg
     3541 gcccaccggg cagtggaaaa attgctggcc ctggtgcagg ttaaagccac tgtcctccgc
     3601 gacggcgccc ccgaagaaat tcccctggag gagattgttc ccggagacgt agtcgcattg
     3661 aaggccgggg ataccattcc cggggactgc gcgatcctgg aagccagaga ccttttcgtg
     3721 aatgaagccg ctctgacggg ggagactttt cccgtagaga aaacccaagg aaagcatccg
     3781 gccgaaacgc ccatgacttt gcggtccaac g
//
LOCUS       DASPRR010000593         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1916582, whole genome shotgun sequence.
ACCESSION   DASPRR010000593 DASPRR010000000
VERSION     DASPRR010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916582"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1398)
                     /locus_tag="VEL68_21775"
     CDS             complement(292..1398)
                     /locus_tag="VEL68_21775"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HXZ38675.1"
                     /translation="MKRNSLRKIIVSGNPLTRGRRYGEKAKSLIGKSVGRYQKAFKEA
                     SGITWKRALDFSRTFVKRIKEYDETLLEEMKGMAKGSGRGFEEILTINLRTEILFGLK
                     KLQPNEGCTSFCALSEITREGNTILGQNWDYKPFAAETMFLLQVNQDTGPDILTLVEA
                     GQLARLGMNSAGVGICNNYIECETDGMNMDKGVPTTLIRRRAFDQETYREVVGMIIHT
                     PRSFSGNYLVATAEGEGDATDIEATPETAYFLFPKDGLIVHSNHIKGAGPGYVGSIRL
                     GLENSLYRDRRLETLLRREVWKIGPDEIMQSLKDHFGYPYSVCRHADKKKPWHDQWQT
                     NASVIMDLTDQVMWISVGPPCQNEYQRFTFDTKD"
     gene            complement(1457..>3085)
                     /locus_tag="VEL68_21780"
     CDS             complement(1457..>3085)
                     /locus_tag="VEL68_21780"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXZ38676.1"
                     /translation="QVNLERFSAPGQTLLGADSHTTTAGGVGMLAIGVGGLDVSVAMA
                     GEPFYLMMPQVMNVRLSGSLPPWSSAKDVALEILRRLTVSGGRGKILEFSGEGVKTLS
                     ATERATIANMSIETGAITALFPSDENTRRYFAAQDRLQDWIELAADPDAAYDGEMGID
                     LARIEPLVARPHSPDNVVPVTSEAGKKVHQVAIGSCTNSSLEDMFRVAAILRGKLVPP
                     EVSLIIAPGSRQVLRSLIESGGLSDMISAGARLMETTCGFCNGVGQAPRSGGISLRTS
                     NRNFQGRSGTPDANLYLVSPETAAASAIAGKITDPRTLGAPVHVHLPQRSYADDRMIV
                     HPAPEGDAVEIVRGPNIPPLPQFVPLPDELEGELLLALGDNITTDDIVPAGANILKLR
                     SNVPAISEYVFRNIDPSFPARAKASGTGFIVGGRNYGQGSSREHAALCPRYLGIRAVI
                     AESFARIHKSNLINFGILPLELANPTDKSGFRPGDKLRLADLKSLISRGSVVPVRNLT
                     QGKTVQTKLDVTPRLAQVLLAGGLLAYARAKLRG"
BASE COUNT          647 a          867 c          895 g          676 t
ORIGIN      
        1 tcgtatccag aggcctcatt cccgctccct tcgaaagatg gataatcttt tgcgaaccca
       61 ttcttccaaa gccaatcata agcaaacagg cctttgaaag caagcgatga ttaagataat
      121 tatcgcataa aaggctatcc ctggttgcgg cttggcgcac gggcacaggg ttttataggg
      181 gggaagccta gacgaggaga cctgggatgt tcgtgcggaa ggttcagggg tggcatcttt
      241 gacccacaaa agccatgaaa ggggggggcc ggcttgattt ttcgcgagcc atcaatcctt
      301 cgtatcgaaa gtgaagcgct gatattcgtt ctggcaggga gggccgacag aaatccacat
      361 gacctgatcg gttaaatcca tgatgaccga ggcgttggtc tgccactggt cgtgccaggg
      421 tttcttctta tccgcatgcc ggcagacgga atagggatag ccgaagtgat ctttcagaga
      481 ttgcataatc tcgtcggggc cgatcttcca tacttctctt cgtaaaagag tttccagccg
      541 ccgatcccgg taaaggctgt tttccagccc cagccggatg gaccccacat agccagggcc
      601 ggctcccttg atgtgattgg aatggacgat gaggccgtct ttgggaaaca gaaaataagc
      661 ggtttccggg gtggcttcga tgtcggtggc atcgccctcc ccctctgccg tggctaccag
      721 gtagttcccc gaaaaagaac ggggggtgtg aatgatcatg ccgacgactt cacgataggt
      781 ctcctgatca aaagcccggc ggcggatgag ggtggtggga acccccttgt ccatgttcat
      841 cccgtctgtt tcgcactcaa tgtagttgtt gcaaattcct accccggcgc tgttcatgcc
      901 caagcgggca agttgtccgg cctccacgag cgtcagaata tccggccccg tgtcctgatt
      961 aacctgcagc aggaacatgg tttctgcggc aaaaggttta taatcccagt tttgccccag
     1021 gatcgtattc ccctcccggg tgatttcgga aagggcgcag aaggaggtac atccctcgtt
     1081 gggctgcaat tttttcaggc cgaaaagaat ctctgtcctc aggttgatgg tcaaaatctc
     1141 ctcaaaaccg cggcccgatc ctttggccat tcctttcatt tcctctagca gagtctcatc
     1201 atactccttg atccgtttca cgaaggtgcg ggaaaaatcc agggctcttt tccaggtgat
     1261 acccgatgcc tccttgaaag ccttctgata cctgcccacg ctttttccga tcagggattt
     1321 ggccttttcc ccataccgcc tgccgcgggt gagcggattt ccggatacga taattttcct
     1381 caaactgttc cttttcataa tctctccttc gagcgctcag gagacagatt tacgcagata
     1441 cacgcagatc ctatgattac cctctcagtt tggcccgggc ataggccagc aggcccccgg
     1501 caagaaggac ctgggccaaa cggggggtaa catccagctt ggtctgaacg gtttttccct
     1561 gagtcaaatt ccggaccggg accacagaac ctcggctaat cagggattta aggtcggcaa
     1621 ggcgaagctt atccccaggc cgaaagcctg acttgtccgt tgggttagcc agttccaggg
     1681 gcaggatccc gaaattgatc aaattggact tatgaatccg agcgaaagac tcagcaatga
     1741 ccgcccggat tccaagatac cgaggacaaa gggcggcatg ttcccggctg gacccctgtc
     1801 cgtaattccg ccctccgaca atgaatccgg tcccggaagc cttggcccgg gccgggaagg
     1861 atgggtcgat gtttcggaag acgtattcgg agatggcagg gacattggac cgcagtttaa
     1921 ggatattagc cccggcgggg acgatatcat cggtggtaat attgtccccc aaggccagga
     1981 ggagttcccc ttccagttcg tccgggagag ggacgaactg gggcaggggg gggatgttgg
     2041 gtccgcggac gatctccacc gcgtctccct cgggggccgg gtgcacaatc atccggtcgt
     2101 cggcgtatga tcgctgcgga aggtgaacat gaaccggcgc tcccagagtg cgcgggtcag
     2161 tgatcttccc cgcgatggct gaggccgcgg cggtctcagg gctgacaagg tagagattcg
     2221 cgtcaggcgt tccgctcctg ccctggaaat tgcggtttga agtccgcagg gagatccctc
     2281 cggatcgggg ggcttggcca accccgttgc agaaaccgca ggtcgtctcc atcaatctcg
     2341 ccccggcgga aatcatgtcc gaaagaccgc cgctttcgat aaggctgcgc aggacctgcc
     2401 ttgagcccgg ggcgatgatc aggctcacct cgggggggac gagctttccc cttaggatcg
     2461 ctgccactcg gaacatgtct tccaaggagg aattggtgca ggacccgatg gccacttggt
     2521 gaaccttttt ccccgcctcg gaggtaacag ggaccacatt gtccggggag tggggccggg
     2581 ccaccagggg ctcgatcctg gctaaatcaa ttcccatttc cccgtcataa gcagcatcgg
     2641 gatcggccgc taattcgatc caatcttgaa gccggtcctg ggcggcgaaa tagcgccggg
     2701 tgttctcatc gctggggaaa agggcggtaa tggccccagt ttcaatgctc atgttggcga
     2761 tggtggcccg ctcggtggcg ctcagggttt tgactccctc cccgctgaat tccaggatct
     2821 ttccccgtcc gccggatact gtgagccgcc ggagaatttc cagggccacg tccttggccg
     2881 aagaccaagg agggagggac ccggatagac ggacattcat gacttggggc atcatcaagt
     2941 agaaaggctc cccggccatg gccacggaca catccaatcc tcccaccccg atggccagca
     3001 ttccgactcc cccagcggtg gtggtatggc tgtccgctcc gagaagggtc tggccgggcg
     3061 ccgaaaagcg ctcaaggttg acctg
//
LOCUS       DASPRR010000594        15414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1112705, whole genome shotgun sequence.
ACCESSION   DASPRR010000594 DASPRR010000000
VERSION     DASPRR010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15414)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112705"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1200
                     /locus_tag="VEL68_21785"
     CDS             310..1200
                     /locus_tag="VEL68_21785"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXZ38677.1"
                     /translation="MKPLKPFSYFDPQDLPTVSKLLIEQGEGAHLLAGGTDLLVRMKK
                     GEISPSTLINLKRVQGLNGIERKPEKALTIGALTPISALVQSSLIQSESPVLAQAAGL
                     LGSPSIRNLATLGGNIGRASPASDMAPPLMVLTARILVEGPGGKRELPLEAIFAGPGK
                     TNLKPGEVMASFRVPKMPPLSAATYLKLGRREGMDLALVGVAVFLKLDEKGETTEARV
                     ALASVAPIPVRARKAEEELLSGPMTEDRVRRAALAAVSDSCPLSDMRASASYRKEMTR
                     VLASRAIEEVRQMARGGIVH"
     gene            1197..1685
                     /locus_tag="VEL68_21790"
     CDS             1197..1685
                     /locus_tag="VEL68_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXZ38678.1"
                     /translation="MKVNINLMVNGEDWELEVHPQTTLLELLRDHLHLTGTKEGCGLG
                     DCGTCIVLMEGQPVNSCLVLAVDADRRKITTVEGLEQNGQLHPLQEKFLEKGAVQCGF
                     CSPAMVLSAKSLLDRNPAPREEEIKEALSGVLCRCGSYKKIIEAVRAAGGAIQEGLGN
                     EK"
     gene            1675..3999
                     /locus_tag="VEL68_21795"
     CDS             1675..3999
                     /locus_tag="VEL68_21795"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXZ38679.1"
                     /translation="MKSEYVGKRVPRVEGLEKVTGRAAYSVDVELPGMLYGAVLRSPL
                     AHARITAIDLSEAWKARGVKAIVTGKDFPFLFGGMLRDQPFLAIDRVRHVGEPVAAVA
                     ATTEAEAREAAGKIKVQYEELPAVFDPREALAEGAPILHEKLGEYVVVGPYKNIPGTN
                     VCTLRTFSLGDIAAGFAQSDEIFEDELYIHTVAHTPMETHAAVAQFIPTEKKYVIWSS
                     TDRPFRLAKELADALGITLNQVRFISTFSGGGFGGKGSMVAEAVALPLARYSKGRPVK
                     VVFSREEELTASQTRHAAYLKLKTGVKKDGTLVARRAEILWDKGAYSSKGPDVGHRGG
                     LTIFGPYRIPHLELFSRMVYTNKQPAGAYRGFGTTQVTWACEVHMDRIARGLKMDPLE
                     FRLKNGYVEGDRYINGQVMDAVGLKETLERAAREIGWGAPKPQASGTRFRGRGLASMI
                     KGTATPTESSCFIKVNPDGSLTLLTSSVEIGAGQKTVLAQIAAETIGVSMDSISIPNP
                     DTAITPYDTGVSSSRTTYHMGNAIQMAGQEIRRKILEVAGEILKTDPAHLSLSRGIIF
                     EENGSERLTLKELFSRKFGGRGGGLVAEGRFSPAGSPLLAAGGKEAMSSIFWMFATHA
                     AEVEVDRETGVVKVLKIVAAHDLGTAINPVGCEQQIEGAAIMGLSNTLLEEFKTERGR
                     ILNDNLADYKLATIKDLPEIVPILVETFHPEGPFGAKGIGEPAAACTAPAIANAIFDA
                     IGIWIKDLPITPEKVLEALRRGKNEGSLETPTPE"
     gene            4053..4394
                     /locus_tag="VEL68_21800"
     CDS             4053..4394
                     /locus_tag="VEL68_21800"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ38680.1"
                     /translation="MKVITIADLSGIVPPDHFDLLSYRIVEPSIGAKAMGASLGRMEP
                     KGRTGPHAHENAEQLFVILKGEMLMKTDRGEARLKAGQAAFIYPGEVHENYNVADGIT
                     EYLVVTSRFGV"
     gene            4764..6302
                     /locus_tag="VEL68_21805"
     CDS             4764..6302
                     /locus_tag="VEL68_21805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38681.1"
                     /translation="MRAKKIWRFWGAVLLLGGILTFGSAPAQAASPAGVLKEAIHWGL
                     SADWLDPATNPGLVPSQITLYLLHDALLKPMPDGTYSPCLAESWSISPDSKVYEFKLR
                     KGVKFHNGDPLTAEDVIFSFQRYKASQAKIIQGKTEKVEAVNSHLVRFTFKEPFPDFL
                     ESLLPGATAIGWVVPKKYVEKVGEAGYKKHPIGAGPYKFVEFVTGVRLVGEAFEEYWR
                     KVPPIKRMEFYVITEPATRLAMARRGEVDIATLMQGVFYEDLKKDPNLRMLPPLSPTR
                     WLVYLASQWDPKSPWADARVRKAASLAIDRKTLADVHMPGCGPIGSIALEGDPMGLDF
                     PADPYDPERAKKLLTEAGYAKGFHGGKFYPYQGGYWPYGEQIANYWKAIGINVDTILL
                     DRPAWFANRQGGKMKGGIFIDNSQAATIGGRLLYLFGSTSYGNYPDVQGLWDQYQREV
                     TPKGRKEMIEHIQRLIYDKTMFIPLTSTNSPAALGPRVKGNPYRIQPLIWFTAPFEDM
                     ELVQ"
     gene            6419..7747
                     /locus_tag="VEL68_21810"
     CDS             6419..7747
                     /locus_tag="VEL68_21810"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXZ38682.1"
                     /translation="MGSQEKSWKVLEGRRVIDGKGGEVPREACVLIEGPRIKAIGAAK
                     DLRIPRGAQVISLPDCTLMPGMMDIHLHTSAYNILTFQNPRAAHFETTPQLQMLYTLL
                     HAQMCFEMGFTTLRNHPWVTAYGGPNTAELVAIRDAIQAGVFAGPRLLVGGHAIITNS
                     HLDLLHPGMALRQPGLTADGPWELRKLVRSQLRTGCDYIKTCASGGGGTEKEEPDIQN
                     MTQEELDAVVDEAHAFHKRCACHCFTPTAQRMAVRAGVDTIEHCVFTDEEAIAMIKAE
                     DKIIVPTLAHRSDRAIEVRRRAGSSEFVLNKMKKIQPYSEETFKKFHQAGIKIALGTD
                     TQIDPEMGTSAHELEVYVDYGMTPMEAIQTGTRNAAEALGLEQETGTLEEGKWADIIA
                     VEGNPLKDIRVLQDKRKIQMVMKEGKVFVFRKPGQEKYVIHDQEWGWKRI"
     gene            7776..8681
                     /locus_tag="VEL68_21815"
     CDS             7776..8681
                     /locus_tag="VEL68_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38683.1"
                     /translation="MEVAKGLSSAREREMTTDLFQLVLNGLLLGGVYALISIGLTLIF
                     GVLEIINFAHGEFLMLAMYATFWLFQLYGVDPYLSIVIILPVFFLIGLSIQRITIQPV
                     LNSPPLNQIFTTIGLSLILQNVALFFFKADYRTVKTSYSDLGLNFAGLIISFPRMVAF
                     ILAMGFIGALLIYLKTTYTGMAIRALAQERRASMLMGIDVYKTYRIAFGIGIACVGAA
                     GSILMPIYFVFPTVGSLFVLIAFVVVILGGYNSLIGSLFGGLIIGIIEGFSGFFISPH
                     LKEAVYFIIFIFILLFKPTGLLGRK"
     gene            8729..10537
                     /locus_tag="VEL68_21820"
     CDS             8729..10537
                     /locus_tag="VEL68_21820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXZ38684.1"
                     /translation="MTAIEKEFPPKSKTISGMIGLALLAVIALPLFIKNEFYLDLIVM
                     IFFWATLAGAWNILGGFAGQISLGHTAYFGIGAYTSTLLYLNFSLSPWIGMLAGVGLS
                     VLVAFVVGYPCFRLSSHFFALATIAFAEVLRMLSAYWRGLTKGGLGLLVPFKPGFEYF
                     MFNNKLAYAYIALTFMLLMIFVTWAIKRSRFGYYLISLREDQDGAESLGVNTHRCKTV
                     ALIISVVFTSVMGTFYAQYFQFIDPEICFSISLSIQLALLSIIGGLGTLLGPVMGAFL
                     LTPIDVLLRGWLGGVFAGLNLIVYGAILIVAVMYFPIGVGGWLKIRWERLLRDRMVTP
                     PIPLKMGTASSLLPSISPGGNKTDDLPLLRVRSFGKHFGGLHAVRNLSFHINRGEIVG
                     LIGPNGAGKTTIFNLISGFYSADSGEMEFQGERITGLAPPHRVCRKGIARTFQIVKPF
                     NNMSLLENIMVGVFCRIKDPWESRKEALRIIDFVGLGRHQFSQASSLTIADRKRLELA
                     RALATKPDLLLLDEVVAGLTPKETTGLMSIIKSISARGVTVLMIEHVMKAVMTLSSRI
                     IVIHHGEKIAEGTPAEVGENKAVIDAYLGKGFRRGK"
     gene            10560..11243
                     /locus_tag="VEL68_21825"
     CDS             10560..11243
                     /locus_tag="VEL68_21825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38685.1"
                     /translation="MTHYGDVRALQNVSFRVEEGQVVSIIGSNGSGKSTTLNTISGVL
                     RCSSGSILFNEEKIDQAFPHQIVDAGIVQVPEGRRLFPYMTVYENLKLGSFNSRGRKT
                     LKESIQEVFGYFPILQERRNQIAGTLSGGEQQMLAIGRGLMAKPKLLMLDEPSLGLAP
                     LVVEQVFETIKRINLQKITILLVEQDVFQALALANWAYVLENGSIMMEGGAEDLLNNP
                     QVKEAYIGV"
     gene            11444..12709
                     /locus_tag="VEL68_21830"
     CDS             11444..12709
                     /locus_tag="VEL68_21830"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38686.1"
                     /translation="MKSKTVKVLVYLVLSLCCIAIPTAGLTQEVIKIGALYPFSGAMA
                     LLGTHNFNGVELATTMVNEKGGIQGKKIVFVKGDVPTAEAARSEAERLISVEKLKVLM
                     GTYSSSLSYVATAVAAKHNVPYWETEAISDPITQRGFKYLFRISPTGSEFGNTAANFC
                     NEIISPKLGIKPKDMRVTLMFEDSLFGTTVGETEQKRLKELGFNLLAVDSYSSRVTDL
                     SSLVMKFKSLNPDIIVACSYLNDAILFQKQMKELKLYVKAFVGSGGGHGILDFAKALG
                     KDSDGIFSSDFPLIKDPSVLDPKLDPPLKTVFERYKAKYGDLPDLHAMSGFTAATVFY
                     QHVLPRALSLDPNDIRKAALEVDIPNGSTHMGWGVKYAGEGHPMMGTNLKAFNVMMQW
                     QGGMNYCVWPKKYAEKEMTMVPLPPWDKR"
     gene            12854..13957
                     /locus_tag="VEL68_21835"
     CDS             12854..13957
                     /locus_tag="VEL68_21835"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HXZ38687.1"
                     /translation="MKTKAAVLYEYRNPLVVEQLDLEGPKAGEVLVEYKAAGICHSDL
                     SILNGIIAMPPLPCIPGHEGAGVVVEAGAGVTRVKAGDHVLAIWVPSCGQCYYCIRHQ
                     PYLCVQRDRARTGVMMDGTSRIKKGTQSIAYMMGVGTFSHYNVLSEKSVLPMDKDIPF
                     EAAAVTGCAVVTGVGAVLNAAKVQPGSSVAVVGIGGVGLNVIQGAVLANATTIIAIDL
                     LDNKLDLARKFGATHTINPGKEDFQEKVNSMTGGIGVDYSFEVIGTPKTAQMAFDIIR
                     RGGTAVMVGIAGAQDTIPLSLLQLAGMEKRIIGCYYGSANMLADLKILLDLYKRGRIK
                     VRELITKDYALEEINKGFDDLKSGGNARGVIKY"
     gene            14444..14809
                     /locus_tag="VEL68_21840"
     CDS             14444..14809
                     /locus_tag="VEL68_21840"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXZ38688.1"
                     /translation="MKIYRTSEYSKMGNPTPGQRYRSDILTGEHQAKDLGGIFGILAP
                     GTSPPYHYHEKRESIIIAISGEAVEVLEGKEFPIKAGDVIFIPPGEKHTTLNRSGGEF
                     RYLEYFTCPPVGKDFVEVK"
BASE COUNT         3666 a         3944 c         4305 g         3499 t
ORIGIN      
        1 gatgcggaag acatcgggag tcgagcgaat tttgctcatt gtcgcagggt ttattctggt
       61 gtaccctaat ccgatagcgg atttgctggg cctgtctctc gccggcggag tggctttcag
      121 gcaatggcag actcgaccga ggaagcagtg atttgtgtca gtgtcagtga ttagggaaga
      181 aaagcacggg tcagcgatcg gcgttcaacc atccgccctt aattaaattt tctggttcct
      241 tgacggcacc ggattttttt tactgacact cacactgaca ctgacactgt tttttataag
      301 gacggaatca tgaaaccatt aaagcccttt tcctattttg atccccagga tttgccgaca
      361 gtaagcaagc tcctgatcga acaaggagag ggagctcacc tccttgcggg gggaacagac
      421 cttctggtac gcatgaagaa aggggagatc agcccatcca ctttgatcaa tctgaagcgc
      481 gttcaggggc taaacgggat cgaaaggaaa ccggaaaaag cgttaaccat cggcgctctg
      541 actcccattt ccgcgctggt gcaatcctct ttgattcaat cggaatctcc ggtcttggcc
      601 caggcggcag ggctgctcgg ctccccttcg attcgcaatt tggccaccct ggggggaaat
      661 atcggcagag cctcccccgc ttccgacatg gcccctcctc tgatggtctt gactgctcgc
      721 atcttggtgg agggccccgg gggaaaaagg gagcttcccc tggaggccat ttttgccggc
      781 ccgggaaaga caaatcttaa gccgggagaa gtgatggctt ctttccgggt accgaaaatg
      841 cctccccttt cggcggcgac ttacttgaag ctcggccggc gggaagggat ggatttggct
      901 ttggtcggag tagctgtctt tcttaagctt gatgaaaagg gagaaacaac agaggcccgg
      961 gtcgccctgg cttccgtggc cccgatcccg gtccgggccc ggaaagccga ggaagagctc
     1021 ctttccggcc ccatgaccga ggaccgggtc cggagagccg ccctggccgc agtgtctgat
     1081 tcatgccccc tctccgatat gcgggccagc gcttcttacc ggaaggagat gacccgggtc
     1141 ttggccagcc gggcaattga ggaggtccgg cagatggcca ggggaggaat cgtccattga
     1201 aagtcaacat caacctaatg gtaaacggcg aggactggga gctcgaggtg cacccccaaa
     1261 ccaccctgct ggagcttctt cgggatcatc tgcatctcac tgggacaaaa gagggctgtg
     1321 gcctgggaga ctgcggaacc tgcatcgtcc ttatggaagg ccaaccggtg aactcctgtc
     1381 tggtgctggc ggtcgatgcc gaccgacgaa agataaccac cgtcgagggg ctggagcaaa
     1441 atggtcagct ccatcccctg caggagaaat ttttggaaaa aggagcggtg cagtgtggtt
     1501 tctgttcccc ggctatggtc ctttccgcca agtcgcttct cgaccggaac cccgccccca
     1561 gggaggaaga gatcaaggaa gcgctttccg gggttctctg tcgttgtggg tcctacaaga
     1621 aaatcataga ggccgttcgg gcggccggcg gagcaataca ggaaggcttg ggaaatgaaa
     1681 agtgagtacg tggggaaaag ggttccccgg gtcgaaggcc tggaaaaggt caccggtcgg
     1741 gcggcctata gcgtggatgt ggagctgcct ggaatgcttt acggggcggt tctgcggagc
     1801 cccctggccc atgcaaggat cacggccatt gacctctcgg aggcctggaa ggcccggggg
     1861 gtcaaggcca tcgttactgg aaaagatttt ccctttctct tcggcggcat gctccgcgac
     1921 cagcctttcc tggccatcga ccgggttcgc cacgtggggg aaccggtggc ggctgtggcg
     1981 gccaccacgg aggccgaggc ccgggaggcg gcgggaaaga tcaaagttca atacgaggaa
     2041 ctaccggctg tcttcgatcc ccgggaagcc ctggccgaag gggccccgat cctccatgaa
     2101 aagctcggag agtacgtcgt cgtcggcccg tataagaaca tcccggggac caacgtctgc
     2161 accctccgca ccttttccct gggagacata gcagcgggct tcgcccagtc cgacgaaatt
     2221 tttgaggatg aattatacat ccatacggtc gcccataccc ccatggagac ccatgcggcc
     2281 gtggcccagt tcatccccac cgaaaagaag tatgtgatct ggtcctccac tgaccggccc
     2341 ttccgtctgg ccaaggagct ggccgatgcc ctgggaatca ccctcaacca ggtacgtttc
     2401 atctctacct tctcgggcgg cggattcggc gggaaggggt ccatggttgc cgaggccgtg
     2461 gctctcccac ttgccaggta cagcaaagga aggccggtga aagtggtctt ctcccgggaa
     2521 gaagaattga ccgcttcgca aacccgccat gccgcctacc tgaagctcaa aacgggggtc
     2581 aagaaggacg ggaccctcgt cgcccggcgg gcggaaatcc tctgggacaa aggagcctat
     2641 tcctctaaag ggcccgatgt gggccatcgg ggagggctga ccatcttcgg accctaccgc
     2701 attccccacc tggaactgtt ctcccgcatg gtgtacacca ataagcagcc ggccggcgcc
     2761 taccggggct tcggcaccac tcaggtgact tgggcttgtg aggtacatat ggaccggatc
     2821 gcccgcgggt taaagatgga cccattagag ttccgcctga aaaacgggta cgtcgaggga
     2881 gaccgctaca tcaacgggca ggtcatggat gccgtgggac tcaaggaaac cctggagaga
     2941 gccgcccggg agatcggttg gggtgcgcct aagccccaag cgtcgggaac ccgattccgg
     3001 ggaaggggcc tggcgtccat gatcaaggga actgcgaccc ccacggaatc ttcgtgtttt
     3061 atcaaagtga atcccgacgg gagtcttacc ctcctcacca gctccgtgga aatcggcgcc
     3121 gggcagaaga cggtcctggc ccagatcgcc gccgagacca tcggtgtgtc catggattcc
     3181 atctccattc ccaacccgga tacggccatc accccctacg atacgggcgt ttcttccagc
     3241 cgcaccacct accatatggg gaatgcgatc caaatggccg gccaagaaat ccggaggaag
     3301 attttggaag tggccgggga aatcctgaaa accgaccccg cccacctgag tctctcccgg
     3361 ggcataattt tcgaagaaaa cgggagtgaa cggttgaccc tgaaggaact tttttccagg
     3421 aaattcgggg gaaggggcgg cggcctggtc gccgagggtc gattctcccc cgcgggctct
     3481 cccctgctgg ccgcgggagg aaaagaggca atgagcagca tcttctggat gttcgccacc
     3541 cacgcggccg aggtggaggt cgatcgggag actggagtgg tcaaggtgct gaaaatcgtg
     3601 gccgctcatg acttgggaac ggccatcaac cccgtagggt gcgaacagca gatcgaaggc
     3661 gcggcgatca tgggtctttc caataccctc ttggaagaat ttaagacgga aaggggccgg
     3721 attttgaatg acaacctggc cgattataaa ttagccacca tcaaggacct tcccgaaatc
     3781 gtccccatcc tcgtggagac cttccacccc gaggggcctt tcggcgccaa gggcatcggg
     3841 gagcctgcgg ccgcttgcac cgcaccggcc attgccaacg ccattttcga tgccattggg
     3901 atttggatca aagaccttcc catcactcca gagaaagttc tggaggcctt acgccgggga
     3961 aaaaatgaag gcagcttaga gacccctacc ccggaatgac aatatatgtt tcccggttgt
     4021 attcgcaagt acaaagagga ggataagaga agatgaaggt catcaccatc gctgaccttt
     4081 ccggaattgt gccgccggat cactttgatc tgcttagcta ccggatcgtg gaacccagca
     4141 tcggggccaa agccatgggg gcttctctgg gccgcatgga gcccaagggc cggaccggcc
     4201 ctcacgccca tgagaatgcg gagcagcttt ttgtgatctt gaagggggaa atgttgatga
     4261 agaccgaccg gggagaagcc aggctgaagg ccggtcaggc ggctttcatt taccccggag
     4321 aggtgcatga gaactacaac gtggctgacg ggatcaccga atacctcgtg gtcacgagcc
     4381 gctttggagt ctgagaaaag gcttcgggaa aagactgatc aggcgcagat gaacgcagat
     4441 agaagcagat tttgtatatg ccaaaaacta aaaatatttc cctcccccct ttgagagact
     4501 gtatcataat ccgttcatgg ttcgacaagc tcaccatgaa cgaatgagcg cagtagaatt
     4561 cagtcacttg gccgttcgtc cgctcggctg agctcgcgac gaagcctgag cctgtcgaag
     4621 ggcgaatggg ggattacgac acagtctctt gacggggagg gcgaggggtg aatcgagaga
     4681 gttctgcgac atctgcagat cgatagtcca agggattctt gtcatttcct gagcgactga
     4741 aaaccatgca aaggagggtt ccaatgagag caaagaaaat ttggcgattt tggggcgcgg
     4801 ttctgctcct tgggggaatc ctcaccttcg gctcagctcc tgcccaggcg gcctctcctg
     4861 ccggcgttct gaaggaagcc atacattggg ggctttccgc cgattggctt gacccggcca
     4921 ccaaccccgg cctcgttcct tcacagatca ccctctacct cctccatgac gcccttttga
     4981 agcccatgcc cgacggcacc tactccccct gcctggccga gtcgtggagc atcagcccgg
     5041 actcgaaggt ctatgaattc aagcttcgca aaggcgtgaa gttccacaat ggggaccccc
     5101 tgaccgccga ggatgtgatc ttcagcttcc agcggtacaa ggcgagccag gcgaaaatca
     5161 tccagggaaa gaccgaaaag gtggaagcgg tgaattccca ccttgtccgc ttcaccttca
     5221 aggagccttt tcccgacttc ctggaatctc ttctcccggg ggcgacggcc atcggctggg
     5281 tggtgcccaa aaaatacgta gagaaggtgg gagaggcggg ctacaaaaag catcccatcg
     5341 gcgcggggcc gtataaattt gtggaatttg tcaccggcgt cagattggtg ggggaagctt
     5401 ttgaagaata ctggcggaaa gtgccgccca tcaagagaat ggagttttat gtcatcaccg
     5461 agcccgccac ccgcctggcc atggccagaa ggggtgaggt cgacattgcc acccttatgc
     5521 agggcgtctt ttatgaggat ttaaaaaaag acccaaacct cagaatgctc ccccctttga
     5581 gcccgacccg gtggctcgtg tacctggcat cccagtggga cccgaaatct ccctgggcgg
     5641 atgcgagagt acggaaggct gcaagcctgg ccatagaccg gaagaccctg gccgatgttc
     5701 acatgcccgg ttgcggcccg atcggatcga tcgccttgga aggagacccg atgggactgg
     5761 attttcccgc cgacccctat gacccggagc gggccaaaaa gcttctgacc gaggcgggat
     5821 atgcgaaggg atttcacggg gggaagtttt acccctacca gggaggatac tggccctatg
     5881 gtgagcagat cgccaactac tggaaggcca tcggcatcaa cgtcgatacg attctcctag
     5941 accggccggc ctggttcgcc aaccggcagg ggggaaagat gaaaggcggc atcttcatcg
     6001 acaattccca ggccgccacc atcggaggac gcctgctcta ccttttcggt tccacctctt
     6061 acggaaatta tcccgacgtc caagggttat gggatcaata ccagcgggaa gtgacgccca
     6121 aggggcggaa ggaaatgatc gagcacatac agaggctcat ttacgacaag accatgttca
     6181 tccccctaac ctcgaccaat tcgcccgcgg ctcttggccc ccgggtgaag gggaacccct
     6241 accggattca gccgctcatc tggttcacgg ccccctttga ggacatggaa cttgtccaat
     6301 aagaaggctt cggacccggc cgaatgttag ctcgcaacgc cgtcgaaggg gcggtttgcg
     6361 aactgccctt acgggcattg ccccacaccc cctttttccg ccaatgggag aaataaagat
     6421 gggaagccaa gaaaagagtt ggaaagtcct ggaagggaga agggttatcg atggaaaggg
     6481 tggcgaggtc ccccgggaag cctgcgtcct catagagggg ccccggatca aagcgattgg
     6541 ggcggcaaag gacctgcgta ttcccagggg tgcacaggtg atttccctgc ccgattgcac
     6601 tctaatgccc ggcatgatgg atatccacct gcacacctcg gcctacaaca tcctcacttt
     6661 ccaaaacccc agggcggccc atttcgaaac aaccccccaa ctccagatgc tttacacgct
     6721 gctccacgcc cagatgtgct tcgagatggg ttttacgact ttgaggaacc acccctgggt
     6781 gaccgcgtac ggcggcccta acacggcgga gctggtggct atccgggacg ccatccaagc
     6841 cggggtcttc gccggaccca ggctcctggt cggcggccat gccatcatca ccaattccca
     6901 cctggacctc ctccatccag ggatggctct ccggcagccc gggttgactg cggacgggcc
     6961 ttgggagctt cgcaagctcg ttcgctctca actgagaacg ggctgtgatt acatcaagac
     7021 ctgcgcctcc ggagggggtg gaacggaaaa agaggaaccc gacatccaga acatgaccca
     7081 ggaggagctg gacgccgtcg tcgacgaagc ccacgccttc cacaagcgtt gcgcctgcca
     7141 ttgtttcacc cccaccgccc agcgcatggc cgtaagagcc ggggtggaca cgatcgagca
     7201 ctgcgtgttc accgatgaag aggccattgc catgataaag gcggaggata aaatcatcgt
     7261 ccctaccctg gcccaccggt ccgaccgggc cattgaggta cggcgaagag ccggcagttc
     7321 ggagttcgtc ctgaataaga tgaagaagat tcaaccctac agcgaagaga ccttcaagaa
     7381 attccaccaa gcagggatca aaatcgccct gggcacggat acccaaatcg accccgaaat
     7441 gggcaccagc gcccatgaac tggaggtcta cgtggattat gggatgaccc ctatggaggc
     7501 catccaaacc ggcacccgca acgccgcaga ggcccttgga ctggagcagg agaccgggac
     7561 gctggaagag gggaaatggg cggatatcat cgccgtggaa gggaacccgc taaaggacat
     7621 ccgggttctg caggacaaaa ggaagataca gatggtcatg aaagaaggaa aggttttcgt
     7681 ttttcgcaaa cccgggcagg aaaaatatgt gattcacgat caggaatggg ggtggaagcg
     7741 aatctagatg ggagttgaga gttggaaatt gagggttgga ggttgcaaag ggtttatcat
     7801 ccgcgaggga acgagagatg accactgatt tgtttcagtt agtactcaat ggacttttgt
     7861 tagggggggt ttatgcttta attagcatcg gccttacttt gattttcggg gttctggaga
     7921 taatcaattt cgctcatggc gaattcttga tgcttgccat gtatgcaacc ttctggttgt
     7981 tccagctcta tggcgtagat ccctatttga gcattgtgat tatccttcct gtattcttcc
     8041 tgattggatt atcaatccaa agaattacca tccagccggt cctgaattca cctcctctga
     8101 accagatctt caccacgatc ggattatcct tgatcctcca gaacgtcgcc ctcttcttct
     8161 tcaaggcgga ttatcggacg gttaagacct cttactccga tcttggccta aatttcgccg
     8221 gattgatcat cagctttccg cgcatggtcg ctttcatttt agccatggga ttcatcggcg
     8281 ccctattaat ttatttaaaa accacgtata ccggcatggc aatccgggcg ttagcccaag
     8341 aaagaagggc ttcgatgtta atgggcatcg atgtctataa gacctaccga attgccttcg
     8401 gcataggcat tgcctgtgtg ggcgccgcag ggtctatttt gatgcccatt tatttcgtct
     8461 ttccaactgt cggttcgcta ttcgtcctga tcgcctttgt agttgtaatt ctgggaggtt
     8521 ataacagtct catagggtct cttttcggag gtcttataat cggaattatc gaggggttta
     8581 gcggcttttt catatctccc catcttaaag aagcggtgta tttcattatt tttatattta
     8641 ttttattgtt taagcctacg ggccttctgg ggcggaagta aaagaagaag cccctgactg
     8701 aaatcaagcc aaaggaaatt gttgggaaat gactgctatt gaaaaagaat tcccccccaa
     8761 atcgaaaact atcagtggga tgattggttt ggccctgttg gccgtgattg ccttaccgct
     8821 ctttataaag aatgaatttt atctggacct cattgttatg atcttctttt gggcgacctt
     8881 ggccggggca tggaatatcc tgggagggtt tgctgggcaa atatccttgg gccataccgc
     8941 ctattttgga atcggagctt acacctctac ccttctatat ttgaatttct cgctctctcc
     9001 ctggatcggt atgttggccg gggttggtct ctcggtactc gtcgcctttg tcgtgggata
     9061 cccatgcttt cggctgagca gccacttttt tgcactggcg acaatcgctt ttgccgaagt
     9121 gttgcgcatg ctttctgctt attggcgtgg attgaccaaa ggagggttag gacttttagt
     9181 accctttaag cccggattcg aatatttcat gttcaataat aaattggctt acgcgtatat
     9241 tgctctcacc tttatgttat taatgatatt tgttacctgg gccattaaaa gatcgcgttt
     9301 cggatactac ctcatcagtc tcagagaaga ccaggacgga gcggaaagtt taggcgtcaa
     9361 tactcatcgc tgcaagacgg ttgcattgat catcagtgtt gtgtttacct ccgtaatggg
     9421 gacattttat gcgcaatatt tccaatttat cgacccagaa atctgttttt caatttcttt
     9481 atcgattcaa ctggctctat tgtcgatcat cggcggactg ggaactttgc tgggaccggt
     9541 gatgggtgca ttcctcctta ctccgatcga tgtcctcctc aggggatggc tggggggagt
     9601 tttcgccggg cttaatttga ttgtatatgg ggcaattctt attgtagcag ttatgtattt
     9661 ccccataggc gttggcggtt ggctcaaaat cagatgggaa cgcctactca gagatcgaat
     9721 ggttacccct ccaatcccat taaaaatggg gacggcttct tctcttctcc cttcgatctc
     9781 gccgggaggt aacaagacag acgaccttcc ccttttgaga gttcgatctt tcgggaaaca
     9841 ttttgggggt ctacatgcag tgcgcaacct atctttccac ataaatagag gagaaattgt
     9901 cggattaata ggaccaaacg gagcgggaaa gaccaccatt ttcaacctga ttagcgggtt
     9961 ctattctgcg gatagcgggg aaatggaatt ccagggggag aggataaccg gcttggcacc
    10021 tcctcataga gtatgtagga aggggatcgc aaggactttc cagatcgtta agcctttcaa
    10081 taacatgtct ctcttggaaa acattatggt cggcgttttt tgccgtataa aggacccatg
    10141 ggaaagtaga aaggaagcat tgagaatcat agactttgtt ggtttaggaa gacaccaatt
    10201 ttcccaggcc tccagtctta ccatcgccga ccgaaaacga ttagaattag cgagggcact
    10261 ggcgacaaaa cctgacttgc tcctattgga cgaagtcgtc gcgggattga ctccgaaaga
    10321 aacgaccggg ctgatgtcga ttatcaagtc tatttccgcc cggggcgtta ccgttttgat
    10381 gatcgaacat gtcatgaagg cagtcatgac cctttccagc cgtattatcg tgattcatca
    10441 tggggagaaa attgcggagg gtactccggc ggaagtgggg gagaataaag ccgtgattga
    10501 cgcctatctg ggcaaaggat ttcgaagagg gaaataaagt gcttacattg gaaaaagttg
    10561 tgactcatta cggtgatgtt cgagcccttc agaatgtttc ttttagggtg gaggaaggac
    10621 aagtggtttc gatcatcggg tcgaatggtt cgggaaagag cactactctt aatactatat
    10681 ccggtgtact tcgttgttct tccgggagta tccttttcaa cgaggagaag attgatcagg
    10741 cattccccca tcaaattgtg gatgctggaa ttgtccaggt tcccgaagga agaaggctct
    10801 tcccgtatat gacggtatac gaaaatttga aattgggatc tttcaattct cgggggagaa
    10861 aaaccttaaa ggaatctata caggaggttt tcggttattt ccctattctg caggaacggc
    10921 ggaatcaaat agctgggacc ttaagcggag gggagcaaca aatgctggcc atagggagag
    10981 gtttgatggc taaacccaaa cttttgatgc tggatgaacc atctcttggc ctggctccat
    11041 tggtagtgga acaagtattc gaaaccataa agcggataaa cctgcaaaaa atcactatcc
    11101 tcttggtgga acaagatgtt tttcaagccc tcgctttggc gaactgggcc tatgtcctgg
    11161 aaaatggttc catcatgatg gaagggggag cagaggacct cctgaataat ccccaagtta
    11221 aagaggccta tatcggagta taattcatag cggatatccg aaattgccgg aaaggcataa
    11281 agggatgagg catctttctc gaaacttgaa actcggcgaa gcaaggttaa gaagctaggg
    11341 ggtgatctct ggcctggatt gcctaaagaa cggattcagc tagaatagga tttaacgccc
    11401 atccggggat atctcaaatt tacttcaaaa agggggactc gaaatgaaaa gcaagacagt
    11461 caaagtcctg gtttatctgg tgttaagtct ttgttgcatt gctattccta ctgctggact
    11521 cacgcaagaa gttatcaaaa tcggggcgct gtatcccttt agcggggcca tggccttgtt
    11581 gggaacgcac aatttcaacg gggtcgaatt ggcaactacc atggtcaacg aaaaaggcgg
    11641 gattcaaggc aagaaaatag tttttgtgaa aggggatgtc cccaccgcgg aggctgccag
    11701 gagtgaagca gaaaggctga taagcgtcga aaagctgaag gtcctcatgg gcacctattc
    11761 cagcagtctc tcttatgtgg ccactgcggt tgcggctaaa cataatgtac cttactggga
    11821 aacggaagcc atttccgacc cgattaccca gagagggttc aagtatcttt tccgaatttc
    11881 cccgacgggg agtgaatttg gaaatacagc ggcaaatttc tgcaatgaga taatctcccc
    11941 caaactgggg ataaagccca aagatatgcg ggttaccctc atgtttgaag actccttatt
    12001 tggaactacg gtgggtgaaa cagaacaaaa acgcctgaaa gagctcggat ttaacctcct
    12061 tgcggtcgat agctatagca gccgcgtgac agacctatcc tccttggtga tgaaattcaa
    12121 atctctgaat ccagacatca ttgtggcgtg ttcttacctt aatgacgcca ttctgttcca
    12181 gaagcagatg aaagaattga aactctatgt gaaagctttt gtggggtctg gggggggaca
    12241 tgggattctt gactttgcca aggccctggg gaaagattcg gacgggattt tcagctccga
    12301 ttttcctcta atcaaagacc cttccgtatt ggaccccaaa ttggaccccc ctttgaaaac
    12361 agttttcgaa cgctataagg ccaaatatgg ggaccttcct gatttgcacg ccatgtcggg
    12421 ctttaccgcc gcaacagttt tttatcaaca cgtgcttccc cgggcacttt ctttggaccc
    12481 caatgatatt cgcaaagcgg cccttgaggt ggatattccg aacgggagta ctcatatggg
    12541 ctggggagtg aaatatgcgg gagagggtca tcccatgatg ggaaccaact taaaagcctt
    12601 taatgtgatg atgcagtggc agggcggcat gaattattgt gtttggccca agaaatatgc
    12661 ggaaaaggag atgaccatgg ttccactgcc tccctgggac aagagataaa tttcctggga
    12721 ggggaaagca cgcagagtta gggtgtgggg gtctgggcct ccacacggca cccctggaat
    12781 taggaggtca tcacggccag aatctttgta atccttgtta acagcggcct aatgtctaaa
    12841 aggagagaaa aagatgaaaa ccaaagcggc ggttctgtat gaataccgaa acccacttgt
    12901 cgtcgaacaa ttggatttgg aaggccccaa ggcgggtgaa gtcttagtcg aatataaggc
    12961 cgcaggaata tgccattctg acctctccat cctcaacggc atcattgcca tgccccccct
    13021 tccctgcatt cccggccatg agggtgcagg agtagtcgtg gaagcaggcg ccggggtaac
    13081 ccgagtaaag gccggagatc atgtgctggc catatgggtt cccagttgcg gccagtgcta
    13141 ttactgcatt cgtcatcagc cctatctctg cgtgcaaagg gacagagccc gaaccggggt
    13201 catgatggac gggacatctc ggatcaaaaa ggggacgcaa tccattgctt acatgatggg
    13261 ggtgggaacg ttttcccact acaacgttct cagcgaaaag agtgtcctcc caatggacaa
    13321 ggacattccc tttgaagcag ccgcggtgac gggttgtgcc gttgttaccg gcgtgggcgc
    13381 ggttctcaat gcggcaaagg tccagccggg cagcagcgtc gcggtagtgg gaatcggagg
    13441 cgtcggcctg aatgtaatcc agggggcagt actggctaac gcgacaacca ttattgccat
    13501 cgacctgctc gataataaac tcgacctggc cagaaaattc ggggctaccc ataccatcaa
    13561 tcctgggaag gaagactttc aagaaaaagt aaacagtatg actgggggaa ttggcgtcga
    13621 ttattccttt gaagtgatcg ggactcccaa aaccgcccag atggctttcg atattattcg
    13681 tagaggaggc acggctgtga tggtgggaat tgccggggcc caagacacca tacccctatc
    13741 cctgcttcaa ctcgcaggga tggagaagcg catcatcggc tgctattatg ggtcggcaaa
    13801 catgctggcc gatctgaaaa tattgttgga cctgtataaa agaggccgga ttaaagttcg
    13861 ggaactgatt accaaggact atgcactgga agaaattaat aaggggttcg atgacctaaa
    13921 atccgggggc aatgcgcgcg gggttatcaa atattaaccc gcggattctg catgggttct
    13981 ttgcccaatc tgggcagggt caagtgggtc atggccctgc cctttccatt cgttaaaaag
    14041 tgagaaatta caatcccttc attcccactc acaattggcg tctgactctg gcggcaatcg
    14101 ccttccccat ttccatagtc gatgaaaatc cgcccaaata ctaggttctg acctttcctt
    14161 ccagaaggaa ttcgatgatg gcccgcaacc cgtcccctct ttttgagaaa ggacctccca
    14221 aacatcgccc ctcggacatt tcgccgtgcg gctcccgagg gtccatgcgc cttaaaatgt
    14281 cataccatac cgtgggctgg aaatttttcg gcaacgattt taccggggat gggaaacccc
    14341 cgtggagagg atgaccaaac tttaagttgc ctggagggag cctatttgtt aggatgggat
    14401 cacttggaaa gttcacctta ctattcctta agggaggcgg aggatgaaga tctataggac
    14461 ctcggaatat tcaaaaatgg gaaatccaac cccgggacaa agataccgga gcgatatcct
    14521 gaccggagaa catcaggcta aggacctcgg cggaattttt ggcatcctgg ctcccggcac
    14581 cagccctcct taccattacc atgaaaagcg ggaatcgatt atcatcgcca tcagcggaga
    14641 agcggttgag gtccttgaag gcaaggagtt tcccatcaaa gccggcgacg tgattttcat
    14701 cccccccggg gaaaaacaca ccacgcttaa taggtcgggc ggggagtttc gctacctgga
    14761 gtactttacc tgtccgccgg tggggaaaga ttttgtggaa gtaaaataat ttcattaagg
    14821 taaacccacg gctctgtcgg gggacccatc aagtttgaca gttccggggt gtttggatcg
    14881 tggcccgggg gcaaggacct tctgggcaaa aagcatcggc cgggtgggcc ctctccttgg
    14941 agcttcgacc ggattccgca gaatgcggcg ggctgatcgg gcgaccggcc ggtcgcccca
    15001 acccggagag aacaccttca tgggggagga cggatggtcg gcccgcccgg tccatgaaat
    15061 ggaagtcgcc tcatgatttt tgcccagaag gtccttgccc ctgggcctgc cacccaatcg
    15121 aaggccacct ctgggtggcg tcccttttaa ttcgctctga gcgcttcact gtttttccta
    15181 cctccggctc ttccggtggt catgattttg attataggtc gcgggatgca gattgcagat
    15241 tcagaattta ggcacaacga tatttttcga ttcaaacccg ggtctgaaat ctgcagttta
    15301 aaaccctcaa tctgagatca aagaatatgg ctgaagaatt tgcattgggt ttcatcggat
    15361 ttggcgaagc gggttttgaa ttggcccggg ggcttcattc cgaaggaata tccc
//
LOCUS       DASPRR010000595         9366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_460783, whole genome shotgun sequence.
ACCESSION   DASPRR010000595 DASPRR010000000
VERSION     DASPRR010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460783"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..993)
                     /locus_tag="VEL68_21845"
     CDS             complement(235..993)
                     /locus_tag="VEL68_21845"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXZ38689.1"
                     /translation="MLLTHQEGPVATIAINRPEALNTLTHELILGLTEELRRWSAEKA
                     VRVIILTGNGDKAFVGGVDVRSMMDLDPAGAEKFITDLHRCFVAVRQSEKMVIASING
                     FALGGGLELVAACDLRIASDRARFGMPEVRVGIPSVIEAACLPRLIGLGRAAEMVYLG
                     EMIDAREALRIGLVNEVVPHEELKEETRQMAEKVLQGGPTAMVLQKKLIAQWMDLSLS
                     DAAEAGIRAFRECYRTAEPKEGMKAFLEKRKPRY"
     gene            complement(1141..2370)
                     /locus_tag="VEL68_21850"
     CDS             complement(1141..2370)
                     /locus_tag="VEL68_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HXZ38690.1"
                     /translation="MKRIAFDNSHLSSLNSLFQEVCMDTFLGYPRPNGRAGTRNYLAV
                     IPTVFCANEVAMAVAKKFKSARPLLHHQGCAQLKPDSDRVTRTLISLGANPNVGGVLL
                     IALGCESVSLPEVYKGLKDRGQKVEKVLIQEIGGMKKAVKRGVELAAKLDQSVSKMKR
                     KPVPASQIAMGIKCGSSDTTSGLVSNPATGKASDLLLAQGGTVIFGETTEFIGAEHLL
                     ARRAVSGEVASRILQIVTSMEDRVKALGFDMRGGNPTPGNIRGGLTTIEEKSLGAICK
                     SGSMPIDGVLEYGESVPGKGLFVLDSPGKEAEIMTGLSAAGANVIVFSTGGGAPQGYP
                     IVPVIKVAGNPAKVRVMKDHIDLDASGVLAGQTTIDEVGQKVYDALLAVGSGKKVRAE
                     ALKYDQTVEIYTTGPTI"
     gene            complement(2395..2931)
                     /locus_tag="VEL68_21855"
     CDS             complement(2395..2931)
                     /locus_tag="VEL68_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXZ38691.1"
                     /translation="MARQEVRFAGFGGQGLLLAGIILGKTACLHSGKQAAQTQSYGTE
                     ARGGASQCNVVIDDQEITYVGVVNPDVFVVMSQEAYDKLIEEVKEGGIVFYDSDLVKI
                     KENPKLRQVSVSSTGSAKELGRQMVANVVMLGAMVEGTKILEADQVKVCLKDSVPPGT
                     EELNLKAFDKGRALFKVS"
     gene            complement(2936..3790)
                     /locus_tag="VEL68_21860"
     CDS             complement(2936..3790)
                     /locus_tag="VEL68_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXZ38692.1"
                     /translation="MSTVGETLRLLHPLVQKYLRKERMPHMACSGCGIGQVTHSVLLA
                     VDELGLTPQNTVWVQGVGCSSRITYSTWAGDNIDAHHGRIFAVATGLKMARPDMNFIL
                     FTGDGDCGAIGGNHFIHACRRNLDVTAILFNNGIYGMTGGQVAPTSPLGAVTTTTPYG
                     NIEDPFDMCQMAVAAGAAYVARWRSTDFKPLVASIKKGIQTKGLAFIEVLLQCPTNFG
                     RYVLKTSSPQEVVKYIKEHSVSQKDAGKLSRDELAGKYLLGEFAHRPERKGIVERYQE
                     FLKAHRKE"
     gene            complement(3787..4947)
                     /locus_tag="VEL68_21865"
     CDS             complement(3787..4947)
                     /locus_tag="VEL68_21865"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXZ38693.1"
                     /translation="MSSGIKPGRYFVQGDEAIAEGAIAAGCRFFAGYPITPSSETSET
                     MARRLPEVGGVCLQMEDELASIHAVGGASVGGLKAMTISSGPGISLKLEGLGWGIKNE
                     IPYVVVDIQRAGPSNGVATRVGQGDLYTVRYGSAGGNNCTIALSPKSTQEAFDLTIEC
                     FNLAEIFRCPVFLLSDETVGHTRELLVIPPEEELKVVKRFKPTVPPEQFVMYPLDTPG
                     VINYPFPNIGEGYGICVSPYTHTSKGYASTAQDIQEKMAKRLVYKITENLDKLVRVEK
                     QKMEDAEFAVIAYGGDARPAMAAVDLARERGMRVGFFRPIILWPFPDGEVLKVAEKVK
                     TILVCELNIDGQLSREVARAAKGKAETYHLGSGGVEPHLPMQVFNEIRRLYR"
     gene            complement(4947..5180)
                     /locus_tag="VEL68_21870"
     CDS             complement(4947..5180)
                     /locus_tag="VEL68_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXZ38694.1"
                     /translation="MAITIIKDFCKGCGFCITNCPKKVYELSAEMNKKGYRLPNPVRV
                     EKCTECGICDLYCPDFAIVLEKTKKKGKGKEAR"
     gene            complement(5430..6098)
                     /locus_tag="VEL68_21875"
     CDS             complement(5430..6098)
                     /locus_tag="VEL68_21875"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXZ38695.1"
                     /translation="MRAIQNRRNLKEAVHQRLKESIVRGEIAAGTKLGETQLAQILGV
                     SRTPLREAINRLEQDGFVEIIPRRGAFVKTQSLREILENLELREVLEGLAVRLAARHA
                     TPKMIRKMKACFQGFSARNVEDSISSYAHQNIRFHNLIIQASQNRKLIAIIRNLFDQM
                     DMVRLHTIVLPGRARKSLSEHSAIIRHIEKGSAQTAEKELRAHIADLRQAVLKLPEFR
                     ANAE"
     gene            6481..8247
                     /locus_tag="VEL68_21880"
     CDS             6481..8247
                     /locus_tag="VEL68_21880"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXZ38696.1"
                     /translation="MAEKGINLSEQDLPAVVDRIKDEILDQYVDRIIHPVETILEIDP
                     LLSEKEILRSLVRNIVQFLGADFASIRIYDSFGEGEPSSPYSPLLGEEILELTPFDRA
                     IASEVIRTQRCYQIPNVFRDPNYQDKEKAREIGVHSMLAVPISLPRFTSQGSDTQGVL
                     QVFYKKEDKVFTPFEVQMAEVLSRRVEFVIARKRILSLQKINLTRDKILEDIYLRLAR
                     REGIKMKDVFNRVIPALADLMKIQRCTLFSVLEDREHVKLEAGFPGAQHGIGKVFSAK
                     EPYIGAIVNQGGPFGDFEHEKIHPSYILITNPQMSHLLPPHLKHYLAAQQINCVLYIP
                     LEVDGVVQYFLAFDAQAHHQQFSEDEIMVFSFLGKELMKGLRLEKMGDILHDFKNPAI
                     AIAGFAKRLQRILSEVPDLPKKEKIQQSMEIILEESSRIQALALTLHGEGKETTLDMT
                     DILKKRFRINEEAVRETNRRNILQVEQELKFPLWIRCFPLHLERVFDNLLNNASQAIP
                     EGGELAIRCYQKDMWAVAEISNTGEISEEEKERFLLGESRGRGLHITTRLVKHMGGKI
                     DIDSRDGRTTFRVELPLAPSPP"
     gene            complement(8325..9173)
                     /locus_tag="VEL68_21885"
     CDS             complement(8325..9173)
                     /locus_tag="VEL68_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38697.1"
                     /translation="MTQAPFLDPLIYDLRGVEYVYGGTHRALQSVSLRVKPGERIAIL
                     GSNGCGKSTLLQILDGLLFPTAGEARAFGQKLSEEKFREASFTAFFRKRVGLLFQSPD
                     IQLFCPTVLDEIAFGPLQLDLPREEVFQKCQEMIRMLNLEKVSPRSPHRLSGGEKKKV
                     ALASVLALNPDVLLLDEPTSGLDPRTQVWLIEVLEELHSLGKTIITATHDLSILEEIA
                     DRAYVMAEDHALVADGPPEEILDDLDLLLRVNLIHEHAHEHEGFIHRHGHLHALPHRH
                     DHPDQH"
     gene            complement(9177..>9366)
                     /locus_tag="VEL68_21890"
     CDS             complement(9177..>9366)
                     /locus_tag="VEL68_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HXZ38698.1"
                     /translation="AGQMATLFKRSLQLSGDVHRAMVARGYQGEVRILSVFHLRPADG
                     LWAGACAGLMAFFLWGGR"
BASE COUNT         2012 a         2719 c         2504 g         2131 t
ORIGIN      
        1 aaccccctgg ggagagatca acttgaaggc cgagtgcccc aaataggtga ttttcactcc
       61 cttgccgatc tctgcggcag gaagggtagg ggagaagaaa agcagagcca gaatgaaaag
      121 aacagtcaaa actccccata cctttttccc catggtctag ccctcctctc atgattaggg
      181 taccaacaat gatcattgat caatggccaa tggtcaatga tcaatgaacg atatttagta
      241 ccttggttta cgtttctcca aaaaggcctt catcccttct ttcggttcag cggttctata
      301 acattcccgg aaagcccgga ttcccgcctc tgccgcatcg ctcaggctca ggtccatcca
      361 ctgggctata agcttcttct gcaggaccat ggccgtgggc ccgccctgga ggaccttttc
      421 agccatctgg cgggtctcct cttttaattc ctcatggggg actacctcgt tgaccagccc
      481 gatgcgcagg gcttcccggg catcgatcat ttctcccaga taaaccatct cagcggcccg
      541 gcctagaccg atcaatcggg gaaggcaagc agcttcgata accgaaggaa ttcccacccg
      601 gacttccggc atgccgaagc gggcacggtc ggaggcgatc ctcaggtcgc aggcagcgac
      661 cagctccagc ccgcccccta gggcaaatcc gtttatggag gcgataacca ttttttccga
      721 ttggcggacg gccacgaagc agcgatgcaa atcggtgatg aatttttccg ccccggccgg
      781 gtccaggtcc atcatggacc tcacatccac ccctcctaca aaagccttat ccccatttcc
      841 agtgagaatg atgactcgga cagccttttc cgcgctccac cgccggagct cctccgtcaa
      901 cccaagaatc agctcatggg taagcgtgtt cagagcttca ggacgattga tcgcgatggt
      961 tgccaccggc ccttcctgat gggttagaag catgaacccc ccacctcaat ttcggatttc
     1021 ggattaaaga atccgcaagt taatcaggct gaggctatat ttcttcactt caactcacaa
     1081 tcaagtgtca ttttgcccgg tttttctttc aatccgcaat ccgcaatccg aaatctgcaa
     1141 ttaaatggtt ggtcccgtcg tgtaaatctc cacggtctga tcgtacttca aggcttccgc
     1201 cctcaccttc ttcccgcttc ccacagccaa aagggcatcg tagaccttct ggcccacttc
     1261 atcgatggtc gtctgccccg cgagaacgcc gctggcgtcg agatcaatat ggtccttcat
     1321 aacccggacc ttggccgggt tgccggccac tttgatgacc ggtacgatcg gatagccctg
     1381 cggcgcgccg cctcctgtgg agaaaacgat gacatttgcg cccgccgcag ataagccggt
     1441 catgatctcg gcttcttttc ccggagagtc caggacgaaa agccccttgc ccggtacgga
     1501 ttcgccgtac tccaggactc cgtcgatggg catagacccc gatttgcaga ttgcccccag
     1561 cgatttctct tcgatcgtgg tcaacccgcc gcggatgttt cctggggtcg gattgccgcc
     1621 acgcatgtcg aatcccagtg ctttcacccg gtcttccatg ctggtcacga tttgaaggat
     1681 tctcgaagcc acctctccgc tcaccgctct gcgcgccagg agatgctcgg ccccaataaa
     1741 ctccgtcgtc tccccgaaaa tgaccgtccc gccctgcgcc aggagaagat cgctggcctt
     1801 tcccgtggcg gggttgctca ccaggcctga ggtcgtgtcg gaagagccgc acttaatccc
     1861 catggcgatt tggcttgcgg gaacaggctt ccttttcatt ttggaaacgg actggtccaa
     1921 tttggcggcc aactcgaccc cccgcttcac ggctttcttc atgcccccga tctcctggat
     1981 cagcactttt tcaacttttt ggccccggtc cttcagccct ttgtatacct ctgggaggga
     2041 aacgctctca cagcccagag cgatgagcag cacgcccccc acgttgggat tggccccaag
     2101 gctgattagc gtccgggtga cccgatccga gtccggcttc agttgggcgc aaccctggtg
     2161 gtggagcagg ggcctggcgc tcttgaattt cttcgccaca gccatggcca cttcgttggc
     2221 gcagaagacc gtgggaatga ccgccaggta gttacgagta ccagcccggc cgtttggcct
     2281 cgggtaaccc agaaaagtgt ccatgcagac ctcctgaaag agggagttga gagatgagag
     2341 atgagagttg tcgaaagcga tccttttcac ctctcacctc ccgcctccca actctcaact
     2401 gactttgaac agcgcccgtc ccttatcaaa cgccttgagg tttaattctt cggtcccggg
     2461 ggggacactg tcttttagac agaccttgac ttggtccgct tccaggattt tggtgccctc
     2521 caccatggcg cccagcatga cgacattcgc caccatctgg cggcccagct ctttggcgga
     2581 tcccgtggag gaaaccgaaa cctgacgtag cttaggattt tctttaatct tgaccaagtc
     2641 ggagtcgtaa aaaacgattc ccccttcctt cacctcttcg attaacttgt cgtaagcctc
     2701 ctgggacatg accacgaaga cgtcggggtt taccaccccc acataagtga tctcctgatc
     2761 gtcgatgacc acgttgcact ggctggctcc tccccgggcc tcggtcccgt aagactgggt
     2821 ctgggccgcc tgtttcccgc tgtgcaagca ggcggtcttc cccaggatga tgccggccag
     2881 gagtaaaccc tgacccccaa atccagcaaa acgaacttcc tggcgagcca tatatctact
     2941 ccttccgatg ggctttcaga aattcttgat accgttccac gattcctttc ctctccgggc
     3001 ggtgggcgaa ttcgcccagg aggtactttc cggccagttc gtcccgactg agctttcccg
     3061 cgtccttctg ggaaacggag tgctctttga tatacttgac cacttcctgg ggggagctag
     3121 tcttcaggac ataccggccg aagttcgtag ggcactggag caggacttcg atgaaggcca
     3181 atcctttggt ctggatgccc tttttgatgg aagccaccag gggcttgaag tcggtggacc
     3241 tccatcttgc cacgtaggcg gccccggcgg ccacagccat ctggcacatg tcgaaggggt
     3301 cttcgatgtt tccgtaggga gtggtggtgg tgaccgctcc caatggagag gtcggggcga
     3361 cctgtccgcc ggtcattccg tagatcccat tgttgaagag gatggcggtt acatccaggt
     3421 tgcggcggca ggcgtggatg aagtggttgc ctccgatggc cccgcagtct ccatccccgg
     3481 tgaagaggat gaagttcatg tccgggcggg ccatcttcag tcctgtggcc acggcaaaaa
     3541 tccggccgtg atgggcgtca atattatctc ccgcccaggt ggagtaagtg attcggctac
     3601 tgcagcccac tccctggacc cacactgtgt tttggggagt caaccccagt tcgtcgaccg
     3661 ccagcaggac ggagtgggtc acctgtccga tcccgcaacc tgaacaggcc atatggggca
     3721 tgcgctcctt gcgcaggtat ttctggacca gcggatgaag aagtctcaaa gtctcgccga
     3781 ccgtgctcat cggtaaagcc tccttatctc attgaagacc tgcatgggaa ggtgaggttc
     3841 tacgcctccg gacccgaggt gataggtctc tgcttttcct ttggccgcgc gggcaacctc
     3901 ccgggacaac tgcccgtcga tgttaagctc gcagaccagg atggttttca ccttttcagc
     3961 caccttgaga acttccccgt cagggaaggg ccagaggatg atggggcgaa agaatcccac
     4021 tctcattccc ctctcccggg cgaggtccac ggcggccatg gccggccgag cgtctccccc
     4081 ataggcgata accgcaaact cggcatcctc catcttctgc ttctccaccc ggaccagttt
     4141 gtcgaggttt tcggtgattt tgtataccag ccgcttggcc atcttctcct gaatgtcctg
     4201 ggccgtgctg gcataccctt tggaggtgtg ggtgtagggg gacacacaga tcccatatcc
     4261 ctcgccgatg ttggggaagg ggtagttgat gactcccggg gtgtccaggg ggtacatcac
     4321 gaactgctcc ggcggtacgg tgggcttgaa gcgcttgacc accttcagtt cctcctccgg
     4381 ggggatgacc aggagctccc gggtgtgtcc cacggtctcg tcggaaagca ggaacaccgg
     4441 gcagcggaag atttccgcga ggttgaagca ttcgatggtt aggtcaaagg cctcctgagt
     4501 cgatttgggg gaaagagcaa tggtacagtt gtttcccccg gcggagccgt accgaaccgt
     4561 gtaaaggtcc ccctggccca cgcgggtggc gacaccgttg cttggaccgg ctctctggat
     4621 atcgacgact acgtaaggga tttcattctt gatcccccag cccagtccct ccagtttcag
     4681 gctgattcct ggcccggagg agatggtcat agccttgagc cctcccacgg acgctcctcc
     4741 aacggcgtga atggaagcga gttcatcttc catttgcaag cagacccctc ccacttcggg
     4801 cagacgccgc gccatcgtct ccgaggtttc ggaagaaggg gtgatgggat acccggcaaa
     4861 aaagcggcaa cccgcagcga tggccccctc ggcaatggcc tcgtcgccct gaacaaaata
     4921 tcttcccggc ttgattcccg aggacatcag cgggcctcct ttccttttcc ttttttcttg
     4981 gttttttcca gaacgatggc gaaatccgga cagtacaggt cacagatgcc gcattcggtg
     5041 catttctcaa cccgcaccgg gttgggcaga cgatagccct ttttattcat ttccgcggat
     5101 agctcgtaga ctttcttcgg gcagttggta atgcagaagc cgcagccctt gcaaaagtct
     5161 ttgatgatcg tgatggccat tcagcaaatc ctccttttct tccgccggat ggacaatcca
     5221 gaattgtata cagaatacaa acagggtcat tttaacgtcc cccctttttg agggtcaagt
     5281 aaaaattcag aatggaaaat ggggaacaca gaatggacag taacgaggca acgccaagcg
     5341 gcgtaggcgc agagagaaaa agaattctgc gtttccaagt gccccctcct ttggtgtccg
     5401 tgcagtccgt ttttccatac cgtaggtctt cattccgcat tcgctcgaaa ttccggcagc
     5461 ttcaggacgg cctggcgtaa atctgcgata tgggcccgca gttctttttc cgctgtttgg
     5521 gccgatccct tttcgatgtg ccggataatg gccgagtgct cggacaaaga cttacgggct
     5581 cgtcccggaa gaacgatggt gtggaggcgg accatgtcca tttgatcgaa gaggttgcgg
     5641 atgatggcga tcaatttccg gttctggctg gcctgaatga tcaggttgtg gaagcggata
     5701 ttctgatggg cataggaaga aatggagtcc tccacatttc gcgccgagaa tccctggaaa
     5761 caggctttca tctttcgaat catcttcggc gtggcgtgcc gggcggccag acgcaccgcc
     5821 agcccttcca ggacttcccg aagctccagg ttttccagaa tctcccggag ggactgcgtt
     5881 ttcacaaaag ccccccggcg ggggatgatt tccacaaatc catcctgctc cagccgattg
     5941 atggcttccc gcagcggagt ccgggagacc cccaatattt gggcgagttg ggtctcgccc
     6001 aacttggttc ccgcagcaat ttccccccgg acaatacttt ccttcaggcg ttgatggacc
     6061 gcctccttta aattgcgccg attttgaatg gctctcatcc gaggccaggc tgctccttcc
     6121 tttcgtattg tataatgtat acaaaaataa aaaatccctg tcaagaccgt ttggccgtca
     6181 tggaagaaga aaatattgcc ctatatttcc ttgaaatttc cgaaggttca tgatacctta
     6241 aacccacttg tatgctcggc ccttcagttg ttcgagcaaa ctctcccgcg tatctcccta
     6301 cgagggatag aaaaggggaa tggttcagaa atttctattg cccagagaga cccggttttc
     6361 gatttcagcg ggtgaatctt tctgggggat cgcgattcaa aaccttccat tctatagcgt
     6421 ccaagggcca tgggtttttg ggaaatcgat ccaaaattgg tccaattgat cggactgtgt
     6481 ttggccgaga aaggaataaa cctgtctgag caggacctcc cggccgtcgt cgatcgaatt
     6541 aaagatgaga ttctggatca atatgtcgat cgaatcatcc atccggtaga aaccatcctg
     6601 gagatcgacc ccctgctcag tgaaaaagaa atcctccgtt ccctggtccg gaatatcgtc
     6661 caatttctgg gagccgattt tgccagcatc cgaatttatg attcctttgg ggagggagaa
     6721 ccctcttctc catattctcc tctcctgggc gaggaaatcc tcgaattgac ccccttcgac
     6781 agggccattg ccagcgaagt catccggacc cagcgctgct accagattcc gaacgtcttt
     6841 agggacccca attatcagga taaggaaaaa gcccgggaga tcggggtcca ttccatgctc
     6901 gccgttccca tctccctgcc gcgatttacc tcccagggtt ccgacaccca gggggtcctc
     6961 caggtcttct acaagaaaga agacaaggtc ttcaccccct ttgaagtgca aatggccgaa
     7021 gtcctttccc gaagggtgga gtttgtcatt gcccggaaga ggatcctctc cctgcaaaag
     7081 atcaacctaa ccagggataa aattctggaa gatatttacc tgcggttggc caggcgggaa
     7141 gggatcaaga tgaaagacgt cttcaatcgg gtgatccccg ccttagccga cctcatgaag
     7201 atccagcgct gcaccctttt ctcggtcctg gaagaccggg aacatgtaaa attggaggcc
     7261 gggttcccgg gggcgcaaca cggaatcggc aaggtctttt ccgcaaagga gccctacatc
     7321 ggggcgattg tgaaccaggg cgggcccttc ggtgattttg agcatgagaa aatacacccc
     7381 tcctacatcc tgatcaccaa tccccagatg agccacctgc tgcctcccca tttaaaacac
     7441 tacctggccg cgcagcagat caattgcgtt ctctatatcc cgctcgaggt ggatggagtc
     7501 gtccagtact ttttggcctt cgatgcccag gcccatcatc agcagttttc cgaagacgag
     7561 atcatggtgt tcagcttcct gggcaaggag ctgatgaaag gccttcgcct ggagaaaatg
     7621 ggcgatatcc tgcacgactt caaaaatccg gccattgcca tcgccgggtt tgccaaacga
     7681 ttacagagga ttctgagcga agttcccgat cttcccaaga aagaaaagat tcaacagtcc
     7741 atggaaatca tcctggagga atcttcccgg atccaggcat tggccttaac tctccacgga
     7801 gaagggaaag aaacgacatt ggacatgacc gatatcctga agaagcgttt ccggattaat
     7861 gaggaagccg ttcgggagac aaaccggaga aatattcttc aggttgaaca ggaattgaaa
     7921 tttcctctgt ggattcggtg ttttcctttg cacctcgaaa gagttttcga taacctcctg
     7981 aacaacgctt cccaggccat cccggaggga ggggagctgg ccatccgctg ctaccagaaa
     8041 gacatgtggg ccgtggccga aatttccaac accggggaga tatcggagga agaaaaagaa
     8101 cggttcctgt tgggggaaag ccgagggcgg ggcctgcata tcaccactcg cctggtcaaa
     8161 catatgggcg gaaaaatcga cattgactcc cgggatggac ggaccacttt ccgcgttgaa
     8221 ttgccgctgg cgccttctcc gccttagaaa agctccatcc atttcttccc ccaccgcctt
     8281 gagtctgaaa aaattcctgg ctcctggcct ctcgcttttc cggatcaatg ctgatcggga
     8341 tggtcgtggc gatggggaag ggcgtgaaga tggccgtggc gatgaatgaa cccttcgtgc
     8401 tcgtgggcgt gttcgtgaat caagttcacc cggagaagga ggtccagatc gtccaggatc
     8461 tcttccgggg gtccatcggc gaccagggca tgatcttcgg ccatgacata ggcccggtcg
     8521 gcgatttcct ccagaatgga gagatcatgg gttgcggtta tgatcgtttt ccccaaagag
     8581 tgcaactcct ccaggacttc aatcagccac acctgagtcc gtgggtccaa tccggaggtg
     8641 ggttcatcca gaagcaagac gtcgggattc agggccagca cagaggccaa ggcgaccttc
     8701 ttcttctccc cgccgctcag ccggtggggg gatcgggggg agaccttttc caggttgagc
     8761 attcggatca tttcctgaca cttttggaat acctcttccc gggggaggtc cagttgcagg
     8821 ggaccgaagg caatctcgtc taagacagtc gggcaaaaaa gctggatatc ggggctctga
     8881 aagaggagcc ccacccgctt tcggaagaag gcggtgaagg aggcctcccg aaatttttct
     8941 tcagagagtt tctgaccgaa ggcccgggcc tctcccgcgg tcggaaacaa caatccatcc
     9001 agaatctgca gtaaagttga tttcccgcac ccgttggacc ccaggatggc aatgcgctcc
     9061 ccgggtttta cccgaaggga gacggattgc aacgcccgat gggttccgcc atagacgtat
     9121 tcaacgccgc gcaggtcgta gatcaaagga tcaaggaagg gggcctgagt catggctcac
     9181 cttcctcccc agagaaaaaa ggccatgagg ccagcacagg ccccggccca gagcccatcc
     9241 gcggggcgca ggtgaaaaac ggaaagaatc cggacctccc cctgataccc ccgggcgacc
     9301 atggcccggt ggacatcccc gctcagctgc aaggagcgtt tgaagagcgt tgccatctgc
     9361 ccggcg
//
LOCUS       DASPRR010000596         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2242661, whole genome shotgun sequence.
ACCESSION   DASPRR010000596 DASPRR010000000
VERSION     DASPRR010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242661"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VEL68_21895"
     CDS             <1..1395
                     /locus_tag="VEL68_21895"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXZ38699.1"
                     /translation="DWKVTAMVWMDREILDLQPGKVEDYFGAAVDIGTTTLAVYLCNL
                     RTGRVVSTGAMMNPQVACGEDVMSRISYAMGNPREGLKRLQEAIIQGLNGLIQSAAQA
                     AQISPRAILEMTVVGNTAMHHIFLGIDPHSLGVSPFPPAVHHSQNIKARDLGLKTHPA
                     AYVHVLPIEAGFVGADNVGVLISETPYQKDEMALTIDIGTNGELVLGNRKKLLSSSCA
                     TGPALEGAHLRFGMRAATGAIERVRINPGTLEVRYRVIGQEKWSDECNPEEVQARGIC
                     GSGIIEVVAEMFRRGVVEKSGRINGNLSTPRLRKIEKGYEFVIAWARETAIGQDITVS
                     TSDVRAVQLAKGALYSGAKIMMKRLGVNRLEKVVLAGGFGSYVDPERAMILGMFPDCD
                     LKEVISVGNAAGDGARIALLNRDKREEADRVAREVEYIELTVYPEFAMEFAEAMAFPH
                     MKDPFPHLEEILKK"
     gene            1444..2214
                     /locus_tag="VEL68_21900"
     CDS             1444..2214
                     /locus_tag="VEL68_21900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXZ38700.1"
                     /translation="MAKSFIQLEDGKLAYLAGGKGKNLMLLHSLNISSDSWEKVFGGL
                     TQNFTVHALDMFGHGDSEKPPKNLLIEDYARSVVQFMDRMKIEKAIVCGNSVGALMAV
                     ELGAIYPQRIEKLVLVGCPARDAWERMERLALSALGFDAQGNPQPLSLTDLKMTYAHP
                     TPELAVWFNQLRGKAGVWVKKTMIAISLYDPFPKFPKIKCPTLILFGDQDILKEKEKE
                     ILRGIKGAKSVILHDAGHVPQMDQPEAFLKEVNQFLRP"
     gene            2421..3107
                     /locus_tag="VEL68_21905"
     CDS             2421..3107
                     /locus_tag="VEL68_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38701.1"
                     /translation="MGTLKTGLSIILFLAGLLSCATPHKVMVMYDRPGTLKPGDRVIW
                     ENQFIGSVGDFQANPSGKTVVPLQVKPDFRQALTDQSRFLIQSDPDRPGNQSIKMVLL
                     ASGGKPLPDGAVMEGSTTFSYLTEKGSRDAQAFPKFLQDGLDRLVKEMDRLSDREWQK
                     ELERQIDAWRRELEKSSEETRRYFQREVFPRLEKSIQDFLQRLKELGRELDGKPLEDK
                     LEKLKGTLQS"
     gene            complement(3274..4275)
                     /gene="epmA"
                     /locus_tag="VEL68_21910"
     CDS             complement(3274..4275)
                     /gene="epmA"
                     /locus_tag="VEL68_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HXZ38702.1"
                     /translation="MDEKRRNNLLRRGKMIQGIRQYFLREDFVEVETPYLVPSPGMEP
                     HLVALEVNFQSPDEEKRKIYLHTSPEYCMKKLLGQGWDKVFQICRVFRDEEVSRTHQV
                     EFTMLEWYRASVDYRKIMKDCEGLLDFLSREILNGSELTYQGKKIDLTPPAERLSVAA
                     AMQLYGKVDIQKNRDGESLLEEARARGFLFEPGAYYSFDEVFFKIFLEAVEPHLGSPK
                     PTILYDYPASMAALARVSPHNLLWAERFELYIAGLELANAFSELVDPVEQRKRFESEQ
                     RLRAVLGKPLYPIDEELLTALSRMPPSAGIALGVDRLAMLFCDVPSIQEVLAFPRSS"
BASE COUNT         1074 a         1177 c         1235 g          986 t
ORIGIN      
        1 gactggaagg tcacagccat ggtctggatg gaccgggaga tcctcgacct ccagcccgga
       61 aaggtggaag attactttgg cgccgccgtg gacatcggca ccaccacgct ggccgtctat
      121 ctctgcaacc tgagaaccgg ccgggtcgtt tccacaggcg cgatgatgaa tccccaggtg
      181 gcctgcgggg aagacgtcat gtcccggatc agctatgcca tgggaaaccc gagggaaggc
      241 ctgaagcgat tgcaggaggc catcatccag ggcttgaacg gcctgatcca aagcgccgcc
      301 caggcagccc agatttctcc ccgggcgatc ctggaaatga ccgtcgtggg caacaccgcc
      361 atgcaccata ttttcctggg aatcgatccc cattccttgg gggtttcccc ctttccgccg
      421 gccgttcatc attctcagaa cataaaggcc agggacttgg gattgaagac tcaccccgcc
      481 gcctacgtgc atgttctccc catcgaagcc ggtttcgtgg gcgcggacaa cgtgggggta
      541 ttgatctcgg aaactcctta ccaaaaggat gagatggctt tgaccattga catcggcact
      601 aatggagagc tggtcctggg taatcggaaa aagctgctct cctcttcctg tgccaccggg
      661 ccggccctgg aaggagcgca cctccgtttc ggcatgcgag cagccactgg cgcaatcgaa
      721 agagtgcgta ttaacccggg aaccctggaa gtgaggtaca gggtcatcgg ccaggagaaa
      781 tggagcgacg aatgcaaccc ggaagaggtc caggcccggg gaatctgcgg gtccggaatc
      841 atcgaagtcg tggcggaaat gttccggaga ggggtggtgg aaaagagcgg caggatcaac
      901 ggaaacctgt ccaccccgcg ccttcgcaag attgaaaaag gctacgagtt tgtcattgcc
      961 tgggcccggg agacggccat cggacaggat attaccgtat ccacctccga tgtgcgggcc
     1021 gtgcaactgg ccaaaggcgc gttgtactcc ggggccaaga tcatgatgaa gcggttggga
     1081 gtgaaccggt tggagaaggt cgtcctggcc ggaggattcg gcagctacgt agacccggag
     1141 cgggccatga tcctgggtat gtttcccgat tgcgacttga aggaagtcat ttcagtcggc
     1201 aatgcagccg gggacggggc ccgtatagcc ttgttaaacc gggataagcg ggaggaggca
     1261 gaccgggtgg cccgcgaagt ggaatatatc gaactgacag tctatcccga attcgccatg
     1321 gaattcgccg aggccatggc cttcccacac atgaaagacc ctttccctca tttggaagaa
     1381 atcctaaaaa aatgatttac aggggcaatt cttgaattac tcccccagcc aacggagacc
     1441 aaaatggcaa aatcattcat ccagctcgaa gatggaaagc tggcctatct agccggcggc
     1501 aaagggaaaa acctcatgct gctacacagc ctgaacattt cttccgactc ctgggaaaaa
     1561 gtcttcggag gattgactca gaatttcacc gttcatgctc tggatatgtt cggccatgga
     1621 gattcggaga aacctcccaa aaatcttctc atcgaggatt acgcccgcag tgtggtccag
     1681 ttcatggacc ggatgaagat cgagaaggcg attgtttgcg ggaactccgt tggcgcgctg
     1741 atggccgttg aattgggagc aatctatcct cagagaatcg aaaaactcgt actcgtcggc
     1801 tgcccggcaa gggatgcttg ggagcggatg gaacggctcg ccctttctgc cctcggcttc
     1861 gacgcccagg gaaatcccca gcctctttcc ctgaccgatt tgaaaatgac ctatgcccat
     1921 cctaccccag aattggccgt ctggttcaat caactccggg gtaaggccgg tgtatgggtc
     1981 aaaaagacca tgatcgccat atccctctat gatccgttcc ccaagttccc caaaatcaaa
     2041 tgccccaccc tgatcctatt cggagaccag gatattctaa aagagaaaga aaaggagatc
     2101 ctcaggggaa ttaagggggc aaaaagcgtt attctccatg acgccgggca cgtgccccag
     2161 atggatcagc cggaagcatt tttgaaagag gtaaatcaat ttttgaggcc ttgatcctgc
     2221 gattttcgag ctgaaatttc aaccccgcaa aaggcaggag cagatgaatt tagatgtcca
     2281 gtctccgatg accaaaagtt aggatataaa aagatttcgt acatgcttag tttgcctatc
     2341 ggggttcgtt aatcggggtt tatcgaatat ttggtggtta gaatgtggcg cttcatttta
     2401 tcaccgacga gtagggaagt atgggtaccc tgaaaactgg gttatcgatc attcttttcc
     2461 tggcgggcct gctgtcttgc gcaacccccc ataaggtgat ggtcatgtac gaccgtccag
     2521 gcactctaaa acccggcgac cgggtgatat gggagaatca attcatcgga tcagtcgggg
     2581 actttcaggc caatccatct ggtaaaacag tcgttcccct gcaggtcaaa cctgacttcc
     2641 gccaagcgct gacggatcag agccgtttcc tgatccagtc ggacccggac cggcccggaa
     2701 atcaatcgat caaaatggtc ctattggcct cgggcggaaa gccccttcct gatggcgcag
     2761 tgatggaggg aagcaccaca ttttcttatc tgaccgaaaa gggatcccgc gatgcacagg
     2821 cgtttcccaa gttcttgcag gatggtctgg accgcttggt caaagagatg gaccgtcttt
     2881 ctgacaggga atggcagaaa gaattggagc gccagatcga cgcttggagg cgggaactcg
     2941 agaagtcgag cgaggaaacc cggcgttatt tccagagaga ggtcttccct agactcgaga
     3001 agtctattca ggatttcctg cagcggctga aggagttggg cagagaactg gacgggaaac
     3061 ctctggaaga taaactggag aaattgaaag gtaccttaca atcttgatct atgggagaag
     3121 attgatgcgg gcaggtctat gtttttttct tgtcgggtgg cgtcccgatc ctcggtaagt
     3181 tctgttaggc cctttctaag aaaaaatcag tccaaatcgg aatcagccca aggaggatgt
     3241 cgatagacaa tttgtctctc taactctgaa ctctcacgaa gacctcggga aggcaagcac
     3301 ctcttgaatg ctgggaacat cgcagaagag catggccaac cggtccactc ccagagcgat
     3361 gccggcagaa ggaggcatgc gggaaagagc agtgagaagt tcttcatcga tgggataaag
     3421 gggttttccc aggaccgccc gaagccgctg ctcggattca aagcgcttcc gctgttcaac
     3481 cgggtcgacc agttcggaaa aggcgttggc caattccagc ccggcaatgt agagttcgaa
     3541 ccgttcggcc cacaagagat tgtgcgggct tacgcgggcc agagcggcca tgctggcggg
     3601 gtaatcataa aggatcgtcg gcttggggct tcccagatgg ggttccactg cttccagaaa
     3661 aatcttgaag aaaacctcat cgaacgaata ataggcaccg ggctcaaaaa ggaatcccct
     3721 ggccctcgcc tcttccagga gagactctcc gtcgcgattc ttctggatat cgaccttccc
     3781 gtagagctgc atggcagcag ccacgctcag gcgttccgcc gggggggtca ggtcgatctt
     3841 tttcccctgg taggtcaatt ccgatccatt cagaatttcc cgggatagaa aatcgagcag
     3901 gccctcgcaa tccttcatga tcttccggta atccacgctg gcccggtacc attccaacat
     3961 ggtgaactcg acctggtggg ttcggctgac ctcttcgtcc cggaagaccc tgcaaatttg
     4021 gaagacctta tcccacccct gacccagtag ctttttcatg caatactcgg gggaagtatg
     4081 caggtatatt ttcctctttt cctcatccgg ggactggaaa ttcacttcca gggccactag
     4141 gtgcggctcc atgccgggtg agggtaccag ataaggggtt tcgacttcca cgaaatcttc
     4201 ccggaggaaa tactgacgga tcccttggat catctttccc cgtcggagga gattatttct
     4261 gcgcttttcg tccatatgct tctgcttcac ccacccataa attagaaggg aattaccccc
     4321 cagcgagctg ggccagaatt cactccgcac cgcgcttttc agtcattaga agcttcccgg
     4381 ttgatcgaat ccctttccga ttacctcttg ggtttcatta ataattataa atgccttggg
     4441 atcaatggtt atgaccaggt ctttcagctt ag
//
LOCUS       DASPRR010000597         6392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2395535, whole genome shotgun sequence.
ACCESSION   DASPRR010000597 DASPRR010000000
VERSION     DASPRR010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395535"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1511)
                     /locus_tag="VEL68_21915"
     CDS             complement(153..1511)
                     /locus_tag="VEL68_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ38703.1"
                     /translation="MKTKGSGISRRDVLKLGAAAAASATLPLVHVRTARAAGKLSVGF
                     WDHWVPAGNAAMKKQVAAWAKKNKVEVTDDYITSIGNKILLTMSAEAQAKAGHDILAF
                     DTWNIGNNAENLEPVDDVINSLEKKYGKINPASEYLAKVKGRWVGVPSSSGSQYKPSC
                     ARISFFKAQGLDVQAMYPAKPEHTALSDQWTWDTMLKLAEPAKKANLPFALGLGQTSD
                     NVDWVGALFRSFGAELVDAQGNITVKSDAVKQALEYMMKLAKFLPDDVYSFDDASNNR
                     ALIAGKTALIFNPPSAWAVAKRDNPSVAADCWTFPTPAGPKGRFVPHLTYFWGIWSFS
                     KNKSAAKDLLTFLMQREQVEERDIAVDGYDIPPFMSMSDFKIWSEVEPPRGTVYNYPV
                     RPFHNATPSISAYPAPRDIAVQIYNRATVTTLVAKMTKGGQSMDQALSWAQDELEGFK
                     RG"
     gene            1812..2132
                     /locus_tag="VEL68_21920"
     CDS             1812..2132
                     /locus_tag="VEL68_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38704.1"
                     /translation="MIPLVNPGEHHRELFSVTIHDHYIGTLRKLLRLIASGWLEAILM
                     DLPRYGVIAPAKNTPLLPASQADISRIFLFPIHPLHSANPAYFRLKQHILGKNPKNKN
                     SGKR"
     gene            complement(2394..4850)
                     /gene="gspD"
                     /locus_tag="VEL68_21925"
     CDS             complement(2394..4850)
                     /gene="gspD"
                     /locus_tag="VEL68_21925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HXZ38705.1"
                     /translation="MKPGKWIHLACLTLFFFLAGTIHCAGTQSVVQLEPSPEPVPKGM
                     GERSKEVAREASPGALKNSPREITRETPNPLQAESFQPQAPKASMKEKTFAQRLEPAP
                     GSAQPSPQPMRPGTLPGVPPPPSVMAAPPVSPPTETPAPIGPSRRAEPPKGGRFVLNF
                     DNADIYEVIRVMAEMMGINYAIDPRVKGVVNIRTTGQISTQDIFPIFQTILKMNGATA
                     VQKGTVYEIVPFGEAKKLFIPPTADRVPGRNQEEKYTIQILTLRFIPAAEASKIIKPF
                     LSDGADIVEHPHQNLLVVGDLSSNIQKALSMMELFDVDIFSEMRVRIYPIYNSDVSDV
                     AKEMERLFTSLEVSVKSGRGVGITFTPIVRNNYLLVVSSIPGIFEKVEKWVRELDRIP
                     GEGSRISVFVYYVQNGKAKDMAEVLKQVYVPTRLRPGTTTTTPTSTPTTPVPGVSPAQ
                     ARGLRPTPAPSVGPEGAAMAGEGGGVTEGEINIVVDETTNSLIIRAYQRDYRRVMETI
                     KKLDIYPKQVLIEVFLAEVTLDDSVKYGLEWSTFSDSFSKGGRTYSYTLGAGGIAPQT
                     DMVSGLRYAITSVDRLAAAISASATEDRLKVISSPHVLASNNKEARIQIGQSQPILTN
                     TYTTTATGTPGVVEGTIEYKDIGIIVTVTPRISDGKLVTLDISVESSTVGKTALGNLS
                     DVPFFPKKVAKTTLSIMESQTIVIGGLIEDRKETIKTGIPYLSKIPVLGALFGYHTNT
                     VNKTETVLFLTPRVISDLADSNRVTEEIREKMFTIQKELERLKKEKEKERGKESSKIE
                     PAAPRVGAAQTRPATEFTSP"
     gene            complement(4892..5710)
                     /locus_tag="VEL68_21930"
     CDS             complement(4892..5710)
                     /locus_tag="VEL68_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38706.1"
                     /translation="MSQRYGIFCVLLFLVVLILAYENYETWSSPGALVFKKEGDKKWE
                     GRPEPPPGVLTPKETPPRQSFDVIAEKNIFNPDRQEFSTQATPGMTKPITRPPIILYG
                     VVIAGDYQMASIVNPGRPLHKGEREIKTIKIGESVGEYKLTKIMPDRIVLEGGEDSFE
                     ILLYDPKSPKKRVEIKTPSLSPAVTSAVPSAPSPEMKPMIPPAAIAPPAATPPTPSPR
                     PVIPTPIPSTPGPQPEGGYQSPTSPPAETVPPSATDPGLWRGRRPAYPPARPPG"
     gene            complement(5710..>6392)
                     /gene="gspM"
                     /locus_tag="VEL68_21935"
     CDS             complement(5710..>6392)
                     /gene="gspM"
                     /locus_tag="VEL68_21935"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HXZ38707.1"
                     /translation="RSGQGKGTFQDQNEDRRPGGDTVKWSLLKKAALSQKKFLWVLIG
                     IAVALAAYFFLLVPLVEATKKADQEMTLNQRILLKYAEVLQSRKAIEEGLARTQKQME
                     EVQKRLIPGETPQLGAANLQDLVKRLAEKNNLGLRSFRNLDPKEVGSYRRISVQIEFN
                     PMSSMQNLTQFLYGLEHQEQKLMISEMDLLVFNPRMPNNVQGNLIISGFMKASEGRAR
                     EKEKGREG"
BASE COUNT         1313 a         1743 c         1773 g         1563 t
ORIGIN      
        1 acaattactg attcaagtct gcctaagaaa ggcagagcca gatggttctg cctttcagct
       61 ttcactgctt gtttcctatt tcttcacttt atcctgtctt ccagtcgccg cgggcgggaa
      121 gcgtcggcgc ctcctctccc cccacgatca catcaaccgc gcttgaagcc ttccagctcg
      181 tcctgggccc agctcagcgc ctggtccatc gactggccgc ccttagtcat cttcgcgacc
      241 aaggtcgtca ccgtggcccg gttgtaaatc tgcacggcga tatcccgcgg cgcggggtaa
      301 gcggagatcg agggcgtcgc attatggaac ggccggaccg gatagttgta taccgtgccg
      361 cgcggcggtt cgacctcgga ccagatcttg aaatcggaca tgctcatgaa aggtgggata
      421 tcatacccgt ccacggcaat gtcgcgctcc tccacctgct cccgctgcat cagaaaggtc
      481 agtaggtcct tcgccgcgct cttgttctta ctgaaagacc agatgcccca gaaatacgtc
      541 aggtggggga caaagcggcc cttcggacca gccggggtcg gaaaggtcca gcaatccgcc
      601 gcgacggacg ggttgtcccg tttcgccacg gcccaggcgg agggcgggtt gaagatcaat
      661 gccgtcttgc cggcgatcag cgcccggttg ttggaggcat catcaaagct gtacacgtca
      721 tcgggcaaga atttagcaag cttcatcatg tattcgagcg cttgcttcac ggcgtctgac
      781 ttcaccgtga tattcccctg cgcatccacc agttcggcgc cgaagcttct gaacagcgct
      841 ccgacccagt ctacgttatc cgaggtttgc ccaaggccta aggcgaaagg caggttggcc
      901 ttcttcgccg gctcggcgag cttcagcatg gtatcccacg tccactggtc ggatagggcc
      961 gtgtgttccg gcttggccgg gtacatggcc tgtacgtcga ggccttgcgc cttaaagaag
     1021 ctgatccgcg cgcaggaggg tttgtactgc gagcctgagc ttgacggcac gccgacccaa
     1081 cgtcctttga ccttggcaag gtattcggaa gctgggttga tcttgccgta ctttttctcc
     1141 aggctattga tcacatcatc caccggctcg aggttctccg cgttgttgcc gatattccag
     1201 gtgtcgaagg ccagaatatc gtggccggcc ttggcctggg cctcggcgga catggtcagg
     1261 agaatcttgt tccctatgga ggtaatgtaa tcgtcggtca cctcgacttt gttcttcttc
     1321 gcccaggccg ccacctgttt tttcatcgcg gcgttgcctg cgggtaccca gtggtcccag
     1381 aacccgaccg agagcttgcc ggcggcgcgg gcggtgcgaa cgtgcaccag cggcagcgta
     1441 gcggaggcgg cggccgcagc ccctagtttc aacacatcac ggcgcgaaat tccagatcct
     1501 ttggtcttca tcggtctccc tccctcgtgt tggttgtaaa cggctgtttc aatttccgaa
     1561 ttggggtcga ttccctcctg agccagagaa acctttgcat aggcgacgag acctcctcac
     1621 ctccttctta cttaattcag gataaggttg aaatgaagaa tgaaggaaga agatggacaa
     1681 aaaatcaaca gaattcacag agccccaaat atcgccaaag caactttttc cagcaaggtt
     1741 ggttctgaaa agatcttttt actcaaaatg tcgatcttgg aagtcgacag taacttgtac
     1801 aaggaaaagg aatgatccct ttggtaaacc ctggagaaca tcaccgtgaa cttttttcag
     1861 tcacaatcca cgaccattat ataggcacct tgcggaaact ccttcgccta attgcctccg
     1921 gatggttaga ggctattttg atggatttac caagatacgg agtgatagca ccggctaaaa
     1981 atactccttt gctcccagcc agtcaagcgg atatttcgcg gatatttctc ttcccaattc
     2041 acccccttca ttcagccaat cctgcttact tccgcttgaa acagcatatt ctcgggaaaa
     2101 accccaaaaa caagaattca ggaaaaaggt aagaccccat tttttgaatt ttcgggcagg
     2161 attgttatcc ggaaaactta aagggtttgg aatcgaaaaa ggattcatga cgcctttgat
     2221 tgactacagt actcattggt ggatttgcca agggggcgca agggaagtcg atgaaaaggg
     2281 actcctctgc ctttaccgga ttcctttcag ccactttaag cagaggtgac gagagaaagg
     2341 ggcagggaaa tgggggccgg accaggcgac ccctagacct cggtttacga aaatcaggga
     2401 gaagtgaatt ccgtggccgg ccgggtctgg gcagctccca ccctgggagc ggcaggttca
     2461 attttggaac tttcttttcc tctctctttt tctttttcct ttttcagcct ctccaattcc
     2521 ttctggatcg taaacatttt ctctcgaatc tcttcggtca cccggttgga atccgccagg
     2581 tcgctgatca cccggggagt cagaaaaagg accgtttcgg tcttgttcac ggtattggtg
     2641 tgatacccga atagcgcgcc caggaccgga attttgctca aataaggaat gcccgtcttt
     2701 atggtttctt ttcggtcttc gatcaggcct ccgataacga tggtctggct ttccatgatg
     2761 gaaagagtgg tcttggcgac cttcttggga aagaaaggaa catccgacaa attccccagg
     2821 gcagttttgc ccacggtgct cgattcaacg gatatatcca gggtcaccag tttgccgtcg
     2881 ctgattcgcg gggtcacggt tacgatgatc ccgatatcct tgtattcgat cgtcccctcc
     2941 accactccgg gcgttccggt tgcagtggtc gtgtaggtat tcgtcagaat gggttgcgat
     3001 tgcccaatct ggatgcgcgc ttctttgttg ttcgaagcca gcacatgggg ggaggagatg
     3061 actttcagcc ggtcctccgt ggccgaagcg ctgatcgccg cagccaaccg gtccactgag
     3121 gtgatggcgt aacggagccc cgaaaccata tccgtctgag gggcgattcc tcctgcgccc
     3181 agggtatagg aataggtccg accgcccttg ctgaaggaat cactgaaagt cgaccattcc
     3241 agcccgtact tcacgctatc gtccagggtc acttcggcca ggaaaacttc gatcagcact
     3301 tgcttgggat atatatccag tttcttgatt gtctccataa cgcgccggta atcgcgctgg
     3361 tacgcgcgga tgatcaggga gttggtggtc tcatccacga cgatgttgat ctccccctcg
     3421 gtcacgcccc ccccctcccc cgccatggca gcaccttccg gaccaacaga aggggccgga
     3481 gtcggcctca acccccgagc ctgggccgga gaaacacccg gcaccggggt tgtcggggtt
     3541 gaagttgggg ttgtcgtagt ggtcccaggt cggaggcgag ttggcacgta gacctgtttg
     3601 agcacctcgg ccatgtcttt ggcctttccg ttctgaacat agtacacgaa gacgctgatg
     3661 cggctcccct cccctggtat ccggtctagc tccctgaccc atttttcaac tttttcgaag
     3721 atccccggga tggaactgac cacgagtaga taattgttcc ggacgatggg ggtgaaggtg
     3781 atgcccaccc ctcgccccga tttcaccgaa acttccaagg aggtgaagag cctttccatc
     3841 tccttggcca catcggaaac gtccgagttg tagatggggt agatgcgcac cctcatttct
     3901 gaaaaaatgt ccacatcgaa gagctccatc atgctcaggg ccttctggat attggaggaa
     3961 aggtccccca caacgaggag attctgatgg gggtgttcga cgatatccgc cccgtcggag
     4021 aggaagggtt tgataatctt gctcgcttct gcggccggaa tgaaccttag ggtgaggatc
     4081 tggatggtgt acttttcttc ctgattccgg cccgggaccc gatccgcagt tgggggaatg
     4141 aagagttttt tggcctcccc gaaggggacg atttcataga cggttccctt ttggaccgcc
     4201 gtcgctccgt tcattttaag gatagtctgg aagatgggga aaatatcttg agtggaaatc
     4261 tgaccggtag tgcggatatt cacgacccct tttactcgcg gatcgatagc gtagtttatt
     4321 cccatcatct cggccatgac ccggatcact tcatagatat cggcgttgtc gaaattgagg
     4381 acaaatctgc cccccttcgg cggttcggcc cgccgggaag gcccaatggg agcgggagtt
     4441 tcggtcggtg gggaaaccgg cggagccgcc attacggagg ggggaggcgg cacgccggga
     4501 agggtcccgg gcctcatcgg ctggggggag ggctgggcgg agccgggcgc cggttcaagc
     4561 ctctgagcga aggtcttttc cttcatggat gctttgggag cttgaggctg aaaggattcc
     4621 gcttgcaagg ggttaggagt ttctcgggtg atttctcgcg gagagttctt gagcgcgcct
     4681 ggggaagctt ctcgggcaac ttccttgctc ctttccccca ttcctttggg aactggttcg
     4741 ggggagggtt ccagctgcac tacggactgg gtcccggcgc aatggatggt tcccgccaga
     4801 aagaagaaga gcgtaaggca tgccaaatgt atccattttc caggtttcat ccccaataag
     4861 ctcctttccg acataggatt tccttcttcc tttaaccagg gggcctggcg gggggatagg
     4921 cgggtcttct ccccctccac aatcctgggt ccgtagccga aggcggaaca gtttccgcgg
     4981 gaggagaggt gggagattga tatcccccct cgggttgcgg accaggggta ctgggaatcg
     5041 gcgtagggat tactggccgg gggctgggag tgggcggtgt cgcagctgga ggagcaatag
     5101 ccgcaggagg aatcattggc ttcatttcag gagaaggagc cgaaggaaca gcggaggtga
     5161 ccgcgggcga aagactagga gtcttgatct ccaccctctt ctttggggac ttcggatcat
     5221 agaggagaat ttcaaaagaa tcctctccgc cctccagcac gatccggtcc ggcatgattt
     5281 tcgtgagctt gtactccccc accgattccc cgatcttaat cgttttgatt tcccgttccc
     5341 ctttgtgcag aggccttcca gggttgacaa tactggccat ctggtagtct ccggcaatca
     5401 ccaccccata caagatgatg gggggtcggg taatgggttt ggtcattcct ggcgttgcct
     5461 gggtggagaa ttcttggcga tccgggttga agatgttttt ttcggcaatc acgtcgaaag
     5521 attggcgggg cggggtttct ttaggggtca ggactcccgg tgggggttcg ggtctgccct
     5581 cccatttttt atccccttct tttttgaaga ccaaggcccc cggggaggac caggtttcat
     5641 aattctcata tgccagaatg agaaccacca aaaaaaggag aacgcaaaaa atgccatatc
     5701 gttgggacat tacccttctc tccccttctc tttctctctg gctctgcctt cgctcgcttt
     5761 catgaacccg gagatgatca ggtttccctg aacgttgttc ggcatgcgag ggttaaaaac
     5821 caggaggtcc atctcgctga tcatcagctt ttgttcctga tgctccagtc cgtagaggaa
     5881 ctgggtcagg ttctgcatgc tgctcatagg attaaattca atctgaaccg aaatcctgcg
     5941 ataggacccc acctctttgg ggtccagatt gcggaaactc ctcaggccca ggttattttt
     6001 ctccgccagg cgtttcacca agtcttggag attggcagcc cccagctggg gggtttcccc
     6061 cgggatgagc cgcttctgga cctcctccat ttgtttctgg gttcgggcca gcccctcttc
     6121 tatggccttg cggctctgga ggacttcagc atacttcaaa agaatccttt ggtttagggt
     6181 catttcctga tccgcctttt tagtggcctc caccaaagga acgagcaaga aaaagtaagc
     6241 agccagagca acggcaatgc cgatgaggac ccagaggaat ttcttctggg ataaggcggc
     6301 cttctttaaa agggaccatt tcacggtgtc ccccccaggc cttcgatcct cattttgatc
     6361 ttgaaacgtt ccctttcctt gtccgctccg ag
//
LOCUS       DASPRR010000598        11278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2243108, whole genome shotgun sequence.
ACCESSION   DASPRR010000598 DASPRR010000000
VERSION     DASPRR010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11278)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243108"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1634
                     /locus_tag="VEL68_21940"
     CDS             273..1634
                     /locus_tag="VEL68_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXZ38708.1"
                     /translation="MTPQTYSTVRGFFLTSASWFIVATLAGFTGAISMVAPDFLQGIP
                     WLVFGRVRPIHTNLMIFGFVGAALLGGVHYFVPSLVRAPLYSEKLGRATLWVYNLAMA
                     SGSITLSLGYTQSREYAEWVWPVDILVVLSLAMIFYNLLRTVLLREEKLLYVSVWYAL
                     AALVFTSLIYFTGNAMWNPSTGAITGMPDAILAWFYGHGIVGLFLTPLAVGVAYYVIP
                     IVSRAPLFSHALSLVGFWSILMFYPHIGTHHLLQTPAPTWLKIVAITGSIGMLIPVQT
                     VLFNLWLTIRGRLGNIHSDVGGKFVFAGLVWYMLVCLQGPLQSLPQVQRLTHLNNWVV
                     AHSHIAVLGFSGFIGLGAVYFTLPRVTGRPLYSSRLADIQYWLILIGLAGFFVVLTIA
                     GLIQGSGWLNGQVVYVILPQVYLYFVLRAALGILIIGGAVIGFYNIVRSLYGTRASEE
                     PSE"
     gene            1631..2578
                     /locus_tag="VEL68_21945"
     CDS             1631..2578
                     /locus_tag="VEL68_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HXZ38709.1"
                     /translation="MNMNLRLLLGGSLIIFAAILCVAVILPWATSIEKPSDIHRPRTS
                     LEEEGRKVYIANGCTYCHTQFVRNLDWDLGAQRIAQAGDYIQEQPHLLGSERTGPDLS
                     QEGGEHPDDWHLAHYTNPRYTRPESIMPSFEFLGREKIEALIAYKQGLGFKDADFRVQ
                     RQRTWKKKAVEAYEGGPDRNVEWLHSHVPEPWTKLPNPYPTTEAGLKRGHKIYQSFCI
                     GCHGPIGDGMGPAQPYLYPPPLNFTLLKGRGISGGILYYQIMNGITGTAMPYFKRELQ
                     SEKIWDVGNYVAVTFIGESDAGAEPRGIAAAYEPPAKEN"
     gene            2582..2851
                     /gene="ccoS"
                     /locus_tag="VEL68_21950"
     CDS             2582..2851
                     /gene="ccoS"
                     /locus_tag="VEL68_21950"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HXZ38710.1"
                     /translation="MYYLPWVFLVVASLWVSLAAFFWGLKSGQFSDQARARYLPLRGE
                     CLSPPLRNPAKARPEVYFLFGILGLGCLILAFTLFLSLGSYFQGR"
     gene            2856..3128
                     /locus_tag="VEL68_21955"
     CDS             2856..3128
                     /locus_tag="VEL68_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38711.1"
                     /translation="MRFFDVLDFQYLILTVFLGAASLFIIYLGFRSYSYSWKERPKSA
                     EDEHEYPGGIRIGTHPIPPLLFFLFIGFGVWFFIYVIFYGILGEPF"
     gene            3195..3479
                     /locus_tag="VEL68_21960"
     CDS             3195..3479
                     /locus_tag="VEL68_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38712.1"
                     /translation="MKSQEHLNEVKRGLRGWLIVGTMALLFLFYGFFMYFVVGDKGPP
                     PWNFGVVEDVPGQSSYSTHPPRQGRGTEPEPQHVSRKPSKILSEVQKEKP"
     gene            3476..3958
                     /locus_tag="VEL68_21965"
     CDS             3476..3958
                     /locus_tag="VEL68_21965"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXZ38713.1"
                     /translation="MTCYPVWLLLLLLLFAPPGCNYGRMNDQESVRTYEEKMPDSPPG
                     TIPISGGVEVLSRTEPKDLKNPVPPTVPAISWGKAAYFNYCVHCHGPKADGNGTVGQS
                     FAPLPSNLNDPQVLDQSDGELFSKISLGFGRHPALANTVSETDRWSIIFYLRSLKGKS
                     "
     gene            4167..4868
                     /locus_tag="VEL68_21970"
     CDS             4167..4868
                     /locus_tag="VEL68_21970"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXZ38714.1"
                     /translation="MVFDFLAPLLIGFAGSLHCLGMCGPLVMAYSLNISAAEKEPGAG
                     ASALWWKGIFHHAAFHLGRILTYGFLGALAASLAQLTDLGRFFPWFRSGITLGGGILM
                     VLLGLFLLKVIPISLFSFNSWGQTSFWRKIFPPLLQSHRQASKLALGLVTGFLPCMLS
                     WAMVVKAATTQNPLLGFLTMAFFGLGTAPALFLPGLSASLLSMRTRFLGERVAASSVI
                     AIGLILIFKGSKYFV"
     gene            4861..7440
                     /locus_tag="VEL68_21975"
     CDS             4861..7440
                     /locus_tag="VEL68_21975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXZ38715.1"
                     /translation="MSESSLLNLSPTPSVSAADKFSIAKGCDLCGLPLGRAKTLQKIG
                     KETFHFCCSGCRQVFIILSSHPDGRPGNFRETDLYRACLLAGIIPGNQEDLTSRDFEI
                     PAAEEGKALQSLNPDLDPNTKQNLSLDLSLQVQGMWCPACCWLIEEVLRRTKGILEAR
                     VSFLSDRVNLRYLPHLLSPTEIMERISKLGYHPSLQLEQEAIALEKKDLLVRLGISAI
                     LTVNIMMVSFALYIGLFRDLSPEVISYFSYPIWLMATPVLLYGGLPILKKGIAGFLYR
                     SPTMESLIFVASLSAYLYSLYRMSQGSLHLYFDTASMLITLVLLGRWIEIHVRGKVAG
                     GMAGLSQLTRQKVRLLDQERERWVSSDGVSPGDSFVVFAGERVPLDGKVLEGRGDMDE
                     SVLTGEARPVHKDPGDEVKAGAFLLSGEINVRTTRTGRESSLGQIIALMQGAIEKKNP
                     AEIMADRITRWFVPTILAIAGATGLYLWLLSSTGDEVFLRSLAVLAIACPCSLGIATP
                     IVKLASVGLAKSQGMLVRDPAALEKMKDLDVLVFDKTGTLTAGHFHLQEMVNDEGTGP
                     GQALPLIASVEVHSDHFLAREVLRKVRGKSLKLEEVEGFESLDGMGVKGRVLGREIFI
                     GNRKLMGWQGLEISAALEKRAEEAETRGMTAVFAGWEGKVQSLLLFGDSLRKGIREMV
                     ECVHARGIKTWVVSGDSRATTESVSRQSGIPEFRSEALPQDKVELIRTLQAAGWRVGM
                     VGDGINDAAALAQADVGFALGAGSNIIQEASDFTLLSSDPGKVLDALELSSKAVGAIR
                     QNLFFAFFYNSFGIPLAMAGWLNPLIAAFAMFASSLTVIGNALRISRGKGSRGEKNDP
                     PPD"
     gene            7539..8090
                     /locus_tag="VEL68_21980"
     CDS             7539..8090
                     /locus_tag="VEL68_21980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXZ38716.1"
                     /translation="MESTLDSKETLNRFLADVERRAFQMAKFATRSRDEALDLVQEAM
                     LQFVRHYSIRPREEWSVLFYRVLRSRITDWYRRTFVRRKILTWLGRPEEEDSEDPLQR
                     MPDTGNPNPSDHVLRQEKRGVLEKAIRALPLRQQQAFLLRAWEGMDLKQTAFIMKCSE
                     GSVKTHYSRAVHTLRGLLEGIQT"
     gene            8087..8470
                     /locus_tag="VEL68_21985"
     CDS             8087..8470
                     /locus_tag="VEL68_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38717.1"
                     /translation="MNPKDPEKLFLESVQDLLNQGAEDLGSQTERRLEEFRGRALMSA
                     QEKHLRFFPVRRWIMVGSFAMATLAAAGLFFWLNTSTETLPTGQIEDLEIITSQERLD
                     FYQNLDFYRWLESKEIRNRQNGKEL"
     gene            8518..8961
                     /locus_tag="VEL68_21990"
     CDS             8518..8961
                     /locus_tag="VEL68_21990"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HXZ38718.1"
                     /translation="MNRYILLLALGMIICSTIPCREVLAGPWEQLSPEEQQTLRSFRG
                     QWDQLTPERQERLRRGAERWQRMTPEQRKQAEERFKRWQELPPQEKEAIRRNYQNFRN
                     LSPEEQERIRRGSQQFQDMPPQQRENLRDQWQKTAPREHRDFRHR"
     gene            9137..9547
                     /locus_tag="VEL68_21995"
     CDS             9137..9547
                     /locus_tag="VEL68_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38719.1"
                     /translation="MKRWLVMGIIVTGMIFALVSRAFAEDRRQGTSVGSLMGPKTPDE
                     YYIYQHGQFPPVLPPAEGVSGATNRPFHDSLSAAPASPGHWDLRRVWVPERRQEVWTW
                     GHFENGYWVNGHSEILVYPGYYEERRVWVGGTGY"
     gene            complement(9621..10457)
                     /locus_tag="VEL68_22000"
     CDS             complement(9621..10457)
                     /locus_tag="VEL68_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXZ38720.1"
                     /translation="MTLSNILKRPYKGVLWIAVWAGLLLVFFSCATVPITERRAIHLV
                     SDQEMTTLSFQEYAKVLQQSKLSHNATEVQMVRNVGARIARATEDLLREKGMAEDLNN
                     YKWEFNLIDDPKTVNAWCMPGGKVAVYTGILPVTKDETGLAVVMGHEIAHAVAKHGNE
                     RMSQGLLAEMGGVALSVALSTQAAATSDLFLSAYGLGAQVGVLLPYSRVQESEADKIG
                     LALMARAGYNPQEAVPFWKRMNEKGGSRPPEFLSTHPAPETRIKEIEAFLPEAMKYYR
                     GK"
     gene            complement(10479..10997)
                     /locus_tag="VEL68_22005"
     CDS             complement(10479..10997)
                     /locus_tag="VEL68_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014231922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXZ38721.1"
                     /translation="MGTHLGWKKIGRLGVFMMILFLGAEASGAWSQTLESKEKEAAHG
                     EYLVIIAGCNDCHTPGFAESGGNRPREEWLTGDRVGWRGPWGTSYPSNLRIYMADISE
                     DGWVKKSRLLKSRPPMPWWVLQTLRDEDTRALYRFIKGLGRKGQVAPSHVPANQEPGT
                     PYILFVPQPPKR"
BASE COUNT         2275 a         3286 c         3116 g         2601 t
ORIGIN      
        1 gaggcgagtg aaacggaata ttggctggat cttgtgattc gattaggatg gatttcaggg
       61 gatgaaataa aaggggtata tgaagaatgc ggtgagttac tcgccatttt caccgccatt
      121 ggcaaaaaat gtaaataatg ggaactcctt ttttttcttt cttgactcca gggatttaga
      181 acttcgtggt gcttgtgttt ctaggcactt cgcacatcga actctaggca ctctaggcac
      241 tccgaactct aggcactttt aagaggtccc ctttgacccc acaaacttat tccacggtcc
      301 gcggcttttt ccttacctcg gcctcctggt tcatcgtggc cacgctggcg ggctttaccg
      361 gagccatttc catggtggcg ccggacttcc tccaagggat tccctggctg gtctttggaa
      421 gggtccgccc aatccatacc aacctgatga ttttcggttt cgtgggggct gcgcttctgg
      481 gaggggtcca ctacttcgtc ccctctctgg tccgggcccc cctgtacagc gaaaagctgg
      541 gacgggcgac gctctgggtc tacaacctgg ccatggcttc cggttcgatc accctatccc
      601 tgggctacac ccagagccgc gagtatgccg agtgggtctg gccggttgat atcttggttg
      661 ttttgtccct ggccatgatc ttctataacc ttctccggac cgttcttctc cgggaggaga
      721 agctcctcta cgtctcggtg tggtatgccc tcgccgccct ggttttcacc tcgctcatct
      781 acttcaccgg aaatgctatg tggaaccctt ccaccggtgc catcaccggc atgcccgacg
      841 ccatcttggc ctggttttac gggcacggca tcgtggggct cttcctcacg cccctcgccg
      901 tgggcgtggc ctactacgtc attcccatcg tcagccgggc ccccctcttc agccatgccc
      961 tgtccctcgt cggattctgg tcgatcctca tgttctaccc ccacatcggc acccatcatc
     1021 tcctccagac cccggcgccc acgtggctga agatcgtcgc catcaccggc agtatcggca
     1081 tgctcatccc cgtccagacc gtcctcttca acctctggct gaccattcgc ggccgcctgg
     1141 gaaacattca ttccgacgtg gggggaaagt tcgtcttcgc cggcctggtg tggtacatgc
     1201 tcgtctgcct ccaggggccc ctccagtcct tgccgcaggt gcagcggctg actcacctga
     1261 acaactgggt ggtggcccac tcgcacatcg ccgtcctggg gttctcaggc ttcatcggtc
     1321 ttggagccgt ttacttcacc ctcccccggg tcaccggccg ccccctctac agttcaaggc
     1381 tggcggacat ccaatactgg ctgatcctca tcgggctcgc cggcttcttc gtggtcctca
     1441 ccatcgccgg cctgattcag gggagcggct ggctgaacgg gcaggtggtt tacgtcatcc
     1501 tgcctcaggt ctacctgtac tttgtcctgc gtgccgccct gggcattctc atcatcggcg
     1561 gggcggtgat cggtttttat aacatcgtga gaagcctgta cggaacccgg gcgagcgagg
     1621 aaccttccga atgaatatga acctgcgcct gctcctaggc ggaagcctga ttatcttcgc
     1681 cgcgatcctc tgcgtggccg tgatccttcc gtgggccacc tcgatcgaga agccctcgga
     1741 catccaccgc ccccgcactt ccttggagga agagggccgg aaggtctata tcgccaacgg
     1801 ctgcacctac tgccacaccc agttcgtccg caacctggac tgggacctag gcgcccaacg
     1861 catagcccag gccggggatt acattcagga gcagccccac ctgctcggtt ccgagcgcac
     1921 cgggccggac ctctcccagg aaggagggga gcaccccgat gactggcact tggctcatta
     1981 taccaacccc cggtacaccc gcccggagtc gatcatgccc tccttcgaat tcctgggaag
     2041 ggagaaaatc gaggcgctca tagcctacaa gcaggggctg gggtttaagg atgccgactt
     2101 ccgggtccag cggcagagaa cctggaagaa gaaggccgtc gaggcctacg aaggcggacc
     2161 ggacaggaat gtggagtggc tccactcgca cgtgccggaa ccttggacca agctgcccaa
     2221 cccctatccc accacggagg cggggttgaa gcgggggcat aagatctacc agagcttttg
     2281 cattggctgt cacggcccga tcggggacgg aatggggccg gcccagccct atctctaccc
     2341 tcctcctcta aactttaccc tcctgaaggg ccgtgggatt tcgggcggga tcctctacta
     2401 ccagatcatg aacgggatta ccggcactgc catgccttac ttcaagaggg agctccaatc
     2461 ggaaaagatc tgggacgtgg ggaattacgt ggctgtcact ttcatcggcg agagcgatgc
     2521 cggggcggaa cccagaggca ttgccgccgc ctatgagcca ccggccaagg agaattgaaa
     2581 catgtattac ctgccttggg tttttttggt cgtggccagc ctttgggtaa gtctggccgc
     2641 cttcttctgg ggattgaaga gcgggcagtt ctccgaccag gcccgggccc gttatcttcc
     2701 cctccgcggc gagtgcctga gtcccccctt gagaaatcca gccaaggccc gcccggaggt
     2761 atacttcctc ttcggaatcc tgggcctggg ttgtttgatc ctggccttca ccctattcct
     2821 ttccctgggc agttattttc aagggaggta gaagcgtgcg cttttttgac gtcctggatt
     2881 tccagtatct gattctgacc gtctttctcg gggcggcctc tcttttcatt atttatctcg
     2941 gcttccggag ctactcgtat tcctggaaag aacggcccaa atcggccgaa gacgagcacg
     3001 aatacccggg gggaataagg atcggaaccc atcccattcc tccacttctg ttcttcctct
     3061 tcatcgggtt cggagtttgg ttttttatct atgtgatctt ttacggcatc ctgggcgagc
     3121 ctttttagcc cgccccttcg atcttcttta ctcccgcacg gaaaagcgga gatgggaaaa
     3181 tagggttcag tgctatgaag agccaagaac atctgaacga ggtaaagcgg ggcctccgcg
     3241 gctggttgat cgtggggacc atggccttgt tgttcctgtt ttacggtttc ttcatgtact
     3301 tcgttgtggg ggataaaggc ccccctccct ggaatttcgg agtggtggaa gacgttcccg
     3361 gccaatcttc ctactctacc catccaccac gccagggcag gggcaccgag cccgaacccc
     3421 agcatgtatc ccggaagcct tccaaaatct tgagcgaggt gcagaaagaa aagccatgac
     3481 ctgctatccc gtatggctcc ttctcctcct tctactcttc gcccccccgg ggtgcaatta
     3541 cggccgcatg aacgaccagg aatcggttcg cacctacgag gagaagatgc ccgacagccc
     3601 cccggggacc attcccatct ccggtggagt tgaggttctt agccggaccg aacccaaaga
     3661 cctcaagaac ccggttcccc cgactgtccc tgcaatctcc tgggggaaag cggcgtactt
     3721 taattactgc gttcattgcc atggccccaa ggcggacggc aacgggaccg tgggccagag
     3781 ttttgctccg ctgccctcga acctgaacga cccgcaggtc ctggatcaga gcgatggaga
     3841 actcttttcc aagatcagct tgggcttcgg gcgtcatccc gccctggcca acacggtttc
     3901 ggaaacggac cgctggtcga ttatctttta tcttcgctcg ctgaagggga agtcttaatg
     3961 gaaagtgcct caagtccgct ccagtgcact caagtgcctt tagttaaata ggccagggtt
     4021 gagattgaat ctcaggtaga gtgaaaaaag tttttccaaa gaacctctaa agctggaaac
     4081 ccgaaaactt gccttcagcc gcctcccccg gttttttgac ttgaggcaat taaggcactt
     4141 gagcgcactc aaggcacttt aaggttatgg tattcgattt cctcgctcct ttgctgatcg
     4201 gctttgccgg aagcctccat tgcctgggga tgtgcggtcc cctagtgatg gcctactcgc
     4261 tcaatatcag cgccgctgaa aaggagcctg gtgccggggc gtcggctctt tggtggaaag
     4321 gcattttcca ccatgccgct tttcacctgg ggagaattct cacctatggt tttctcgggg
     4381 cgctggctgc tagtctcgcg cagctcaccg acttgggccg atttttccct tggttccgca
     4441 gcgggataac cctggggggc gggatcctga tggttcttct cggcctgttt ctgctgaaag
     4501 tcatccctat ctcgctcttc tcttttaact cttgggggca gacatccttc tggcgaaaaa
     4561 tctttccccc cctgctccaa tcccatcgcc aggcttctaa attggctctc ggattggtaa
     4621 ccggtttttt accctgcatg ctctcctggg ctatggttgt gaaagccgca accacccaga
     4681 atccactgct aggatttctg actatggcct tcttcggact gggtaccgct cctgctctgt
     4741 ttttacccgg gctctctgct tctcttctat ccatgaggac aaggtttcta ggcgagcggg
     4801 tggccgcttc ctcggtcatc gccataggtc tcatattgat cttcaaaggg tccaagtact
     4861 ttgtctgaat ccagccttct gaatctatct cctaccccct cggtttcggc cgccgataaa
     4921 ttttccatcg cgaaaggttg cgacctttgc ggcctgcccc taggccgggc caaaaccctg
     4981 cagaagatcg gcaaggagac ttttcatttt tgctgctcgg gttgccgtca ggtttttatc
     5041 atcctctcca gccatccgga cggaagaccg gggaacttcc gagaaaccga cctctaccgt
     5101 gcttgccttc tggccggaat tatccccgga aatcaagaag atttaacctc cagagacttt
     5161 gaaattcctg cggcagaaga aggtaaggct ctgcaatccc tcaaccccga cctggacccc
     5221 aacaccaagc agaacctcag ccttgacctc agccttcagg tccaaggaat gtggtgtcca
     5281 gcctgttgct ggctgatcga agaggttctg cggaggacca agggaatcct ggaggcgagg
     5341 gtttctttcc tctccgaccg ggtgaacctg aggtacctgc ctcacctgct ctccccgaca
     5401 gagatcatgg agaggatttc aaagctgggt taccatccat ccctccagct ggagcaggaa
     5461 gcgattgccc tggagaaaaa ggacctgctg gtacggttgg gaatctctgc catcctcacc
     5521 gtcaacatca tgatggtttc ctttgccctt tatatcggcc tttttcggga cttgtcccct
     5581 gaggtgatca gctatttttc ctacccgatc tggctgatgg ctacgccggt tcttctctat
     5641 ggcggactcc caatcctgaa aaaagggatc gcaggcttcc tctaccggag tccgaccatg
     5701 gagtcactga tattcgtagc ctccctttcg gcctatttat atagcctcta ccgcatgtcc
     5761 cagggcagtc tccatctcta tttcgatacg gcttccatgc tcatcaccct tgtgcttctg
     5821 gggaggtgga tcgaaatcca tgtccgggga aaggtggcag gcgggatggc tggccttagc
     5881 cagttgacac gacagaaggt tcgtcttcta gaccaggaaa gggagagatg ggtttcctcc
     5941 gatggtgtaa gtccagggga ttccttcgtc gttttcgccg gagaaagggt tcctttggac
     6001 gggaaagtcc tcgagggacg gggagatatg gatgaatcgg tccttacggg cgaagctcgg
     6061 ccggtacaca aggaccccgg agacgaggtc aaggcgggag cttttctctt aagcggagag
     6121 atcaatgttc ggaccacccg gaccggtcgg gagagctctc tggggcaaat aatcgccctg
     6181 atgcaagggg ctatagaaaa gaagaatccc gcggagatca tggccgaccg gatcacccgc
     6241 tggttcgttc caaccatcct ggccattgcc ggtgccacgg gtttgtacct ctggctcctc
     6301 tcctccactg gggatgaagt attcctccgc agtcttgcag tcctggcgat cgcgtgtccc
     6361 tgttctctgg gaatcgccac ccccatcgtg aagttggcgt ccgtgggatt ggccaaatcc
     6421 caagggatgc tggtgaggga cccagccgca ctggaaaaaa tgaaagacct ggatgttctg
     6481 gtctttgata aaaccggaac cctcacggca ggtcattttc acctccagga aatggtgaac
     6541 gatgagggga cggggccggg ccaggcactt cctctcattg cctcggtgga agttcactcc
     6601 gatcattttc tggcccggga ggttttgcga aaggtccggg gaaaatcatt gaagctggag
     6661 gaagtggagg gatttgaatc cctggatgga atgggggtga aaggccgggt gctaggccga
     6721 gaaatcttta tcgggaaccg aaagctcatg ggctggcagg gcctggaaat atctgctgcc
     6781 ctggagaaga gagcggagga ggcggaaacc cgggggatga cggcggtctt cgctggctgg
     6841 gaagggaagg tccaatctct ccttcttttt ggcgactcac tccgaaaggg aatccgggag
     6901 atggtcgagt gtgttcatgc ccgggggatc aaaacttggg tcgtttccgg ggattcgcga
     6961 gcgaccaccg aatcggtgtc ccggcaatcc gggatccccg agttccgcag cgaagccctt
     7021 ccgcaggata aggttgaact gatcaggacc ctccaagcgg cgggttggcg cgtggggatg
     7081 gtcggggacg ggatcaacga tgccgcagct ctcgcccagg ccgatgtggg tttcgccctg
     7141 ggagccgggt cgaatatcat ccaggaggca tccgatttta ccctgctctc ttccgatccg
     7201 ggtaaagtcc tcgatgcctt ggaattgtcg tccaaagccg tcggggccat tcgccaaaac
     7261 ctttttttcg ccttctttta caactccttc ggaatccccc tggccatggc tggatggctg
     7321 aatcccctta tcgccgcctt cgccatgttt gccagcagcc ttacggtcat cgggaatgct
     7381 ttgcgaatct cgagaggaaa ggggtcccga ggggagaaaa acgaccctcc ccccgattga
     7441 aggaatcgga aggggtcaat ataggaaaat tcgcgaaata gccggtaaac caatttaaag
     7501 gctgtgcgtt atccatagca ggcagcgaga tcgttctcat ggagtcaact ctggattcaa
     7561 aggagacgct gaaccggttt ttggccgatg ttgaacgccg tgcattccag atggccaaat
     7621 tcgccacgcg aagccgtgat gaggccctcg acctggttca agaggcgatg ctccagtttg
     7681 tgcggcacta ctcgattcgt ccccgtgagg aatggagtgt tctcttctac cgggtcctgc
     7741 ggagccggat caccgattgg tatcgtcgga cttttgtccg ccgaaaaatc ctcacttggt
     7801 tgggccgacc tgaagaagaa gactcggaag accctcttca aagaatgccc gacacgggaa
     7861 atcccaaccc ttccgatcat gtccttcgac aggaaaaaag gggcgtcctg gagaaagcca
     7921 tccgggctct tcccttacgg cagcaacagg ccttcctcct gcgagcctgg gaagggatgg
     7981 acttgaagca gacggccttc atcatgaaat gttcggaagg aagtgtaaaa acccattact
     8041 cccgcgcggt ccataccctg cgcggcctgc tggagggcat acagacatga atcccaaaga
     8101 tcccgaaaag ctctttttgg aaagtgtcca agacctttta aaccaagggg ccgaggacct
     8161 cggaagccag actgagcggc ggctggaaga gttccggggc agagccttga tgtccgccca
     8221 agaaaagcac ttgaggttct tccctgtgcg gcgttggatc atggtcggca gcttcgccat
     8281 ggccacgctg gcagcagcgg gcctgttttt ctggctgaat acttccaccg aaaccctccc
     8341 cacggggcag atcgaagacc tcgaaattat cacctctcaa gagcggcttg atttttatca
     8401 aaacctggat ttttatcgct ggctggagag caaagaaatc cggaataggc agaacggaaa
     8461 agaactgtaa ccctggaagg cgaacgaacc caaaatgaaa acctggggaa gggggagatg
     8521 aacagataca ttttgctctt ggccttgggg atgatcattt gttcaacgat cccctgccgc
     8581 gaagtccttg ccggtccgtg ggaacagctc agcccggaag aacagcagac cctgcggtcc
     8641 ttccgtgggc aatgggatca gttgaccccc gaaaggcagg agcgcctccg ccgaggagcg
     8701 gaacgatggc agcgcatgac tcccgagcag cggaagcagg cggaggagcg tttcaagcgg
     8761 tggcaggagc ttcctcccca ggaaaaagag gccatccgga ggaattacca aaatttccgc
     8821 aatctgtctc cggaggaaca ggaaagaatc cggcgcgggt cccagcagtt ccaggatatg
     8881 cccccccagc agcgggagaa tcttcgcgat caatggcaga agaccgctcc ccgcgaacac
     8941 cgcgacttca ggcaccgcta gaagtctacc tatccatccc tgccggaaac agcgatgcct
     9001 tcccaagaaa aacggaagag tcccccggcc ctgtcaactg gtgagggggt cggcgcgttc
     9061 atcaaattga agggaaggtt gccatagggc tcactgacat ttgccccttc gcctagtctc
     9121 aggaggtgat ggttccatga aacgctggtt ggtcatgggt ataatcgtaa ctgggatgat
     9181 tttcgccctg gtcagccggg ccttcgccga ggaccgccgg caaggaacct cagtgggaag
     9241 cctcatgggg ccgaaaacgc cggacgagta ttacatctac cagcacgggc aatttccgcc
     9301 ggttctgcct cccgccgagg gcgtttctgg ggccacgaat cgacctttcc atgattccct
     9361 ctctgcggct ccagcatcgc caggccattg ggacttgcgc cgggtgtggg tgccagaacg
     9421 acggcaagaa gtgtggacct gggggcactt cgagaacgga tattgggtca acggacactc
     9481 cgagatcctg gtttatcccg ggtactatga agaacgaagg gtctgggtcg gcgggacggg
     9541 gtattaatcc gaagaaaaaa aatcatggcc tccggcagag ccgggtcttt ttcctcgcct
     9601 tgcgctggcg cgactggcga tcatttcccc cggtaatact tcatggcctc gggaagaaag
     9661 gcttcgatct ccttgatgcg ggtctccgga gcggggtgag tggaaaggaa ttccgggggc
     9721 cggcttccgc ctttttcatt cattcttttc cagaaaggaa ccgcttcttg gggattatag
     9781 ccggctctgg ccatcagggc caggccgatc ttgtcggcct ccgactcctg gacccggctg
     9841 tatggaagga ggaccccgac ctgggctccc aggccgtagg cggatagaaa tagatcgctc
     9901 gtagccgccg cctgggtgga tagggcgacg gacagggcca ctccccccat ctcggccagg
     9961 agcccctggc tcattctttc gttcccgtgc ttggccaccg cgtgggcgat ttcgtgcccc
    10021 atgaccacgg ccaagccggt ctcgtccttg gtgacgggca ggatccccgt gtataccgcc
    10081 accttccccc cgggcatgca ccaggcgttg accgtcttgg ggtcgtcgat caggttaaac
    10141 tcccatttat aattattgag atcctccgcc attccctttt cccgcagaag atcttcggtc
    10201 gccctggcaa tcctggcccc cacatttctc accatttgaa cttcggtggc gttatgggac
    10261 agcttcgact gttggaggac cttggcgtat tcttggaagc tcagggtggt catttcctgg
    10321 tcggatacca gatgaatggc cctcctctcc gtaatgggta ccgtggcgca ggaaaagaaa
    10381 actaggagaa ggccggccca gacggcaatc caaagaacgc ctttatatgg ccttttcaaa
    10441 atgttggata gggtcatcgg ctcaaatcct cctttcattc acctcttcgg aggctgggga
    10501 acgaacagga tataaggagt cccgggttcc tgattcgccg gaacatggga aggagccacc
    10561 tgtcctttgc gacccaggcc cttgatgaac ctgtacagag cccgcgtatc ttcatctcta
    10621 agggtttgga ggacccacca gggcataggg ggcctggact tgaggagacg gcttttcttt
    10681 acccagccgt cctctgaaat atccgccatg tagatgcgca ggttgctggg atagcttgtc
    10741 ccccagggac cccgccagcc tacccggtct ccggtcagcc attcttcgcg gggacggttc
    10801 ccacccgatt ccgcaaaccc gggggtgtgg cagtcgttgc agcccgcgat gatgaccagg
    10861 tattccccat gcgcagcctc tttttctttg ctctcaagtg tttggctcca cgctcccgag
    10921 gcctccgccc ctagaaatag gatcatcatg aaaactccca atcttccgat tttcttccac
    10981 cccaggtgag ttcccatctc cccctccttt ttaaatgcaa gtcaggcccc tgcctggctt
    11041 tcctcaaaga acttcagccg gagcccaatc gattcggaaa cacccatcaa cgctacaaaa
    11101 aacataccac ccctataaga atgaattccc atcttcccca cggtgattag gaagaagtgc
    11161 aggcttctcc gtcagtcctt cagaaacgcc tttttcatcc aggatcaaaa gattttattt
    11221 ttttatctgt ttatttgatt cttcttggat gtcttcgagg atgatgcctc ctgcccgt
//
LOCUS       DASPRR010000599         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1762924, whole genome shotgun sequence.
ACCESSION   DASPRR010000599 DASPRR010000000
VERSION     DASPRR010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762924"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VEL68_22010"
     CDS             <1..964
                     /locus_tag="VEL68_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38722.1"
                     /translation="TGFEYTLNYYIIKDINAPLLYITLTRGAHLLTAMDVHLEDNHPS
                     IKSMILEHRKDQGPLRIRMKHLPESKIREIEDILFTTERPVQERFYQGRGVNFRTGDI
                     ELWPTEYYLCGGHGMTGIFVNEKAATTLPGLYAAGDVSLVARGHLSGAFVYGEIAAEE
                     SSEYSRKNPPVRGVETPGLTRLKMDLLQRMEQKGGEVSVEEFEYKVRRLISDYVTPPK
                     NAYKLNMALWWMDRFRKEIREKVRVRNIHDLFKVLEVENIILCASLSATASLERKESR
                     WGFWHYRADFPKKDDENWRKHIMLSTGGDPEEVRVSHRPIARMQ"
     gene            974..>2095
                     /locus_tag="VEL68_22015"
     CDS             974..>2095
                     /locus_tag="VEL68_22015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXZ38723.1"
                     /translation="MDFNLLTSLNDNIRIDRNKCTACGRCVEVCVLDNLRLQLSPCRQ
                     ACPLGMNCQGYIHHLAQGKMDKGLEKVREAVPFGGILGRICSRPCEAVCNRVKVDQQP
                     VAIRDLKRYLADQEAGDWAPPRAPERPQKVAVVGAGPAGLTAAFYLRSWGFQVTLYDR
                     ESAPGGLMRWAIPEFRLPSEVFNRELGFLKDMGIDFQGNRTLGKDLDLGKLEEECGAV
                     LLALGAYGQTRLGVPGEEAQGVLQALDFMKNVREKKPPRVGPRVIVVGGGNAAVDAAQ
                     TALRLGARKVHLVSLEKREEMPAFRWSVAEAEEEGVDIQNGWGPMRFRMNGKKLAGVS
                     FKKCAAVFDPQGIISPSYDEKTTLDLPADTVILAVGQRAE"
BASE COUNT          480 a          587 c          648 g          380 t
ORIGIN      
        1 gaccgggttc gaatacaccc tgaactacta tatcatcaag gacatcaacg cccctctcct
       61 ttacatcacc ctgacccggg gggctcacct tctcactgcc atggatgtgc atctggagga
      121 caaccatcct tccatcaagt ccatgatcct ggaacatcgc aaagaccagg gacctttgcg
      181 tatccggatg aaacacctcc ccgagtcgaa gattcgggag atcgaagaca tcctctttac
      241 caccgagcgc ccggtgcagg agcgcttcta tcagggccgg ggagtaaatt tccggaccgg
      301 ggatatcgaa ctctggccta cggagtatta cctctgcgga ggtcacggca tgaccggaat
      361 cttcgtgaac gaaaaggccg ccaccaccct ccccgggctc tatgccgcgg gagacgtctc
      421 cctggttgcc cgagggcacc tgagcggggc ctttgtctac ggcgagatcg ccgccgaaga
      481 atcttctgaa tactcccgaa aaaatccccc tgtccgggga gtcgagacgc cgggcctgac
      541 cagactgaaa atggacctcc tccagcgtat ggaacagaaa ggaggggagg tttccgtcga
      601 ggagtttgaa tataaagtga ggcgactgat cagcgactac gtgaccccgc ccaagaatgc
      661 ctacaagctg aatatggccc tctggtggat ggatcggttc cggaaagaaa tccgggagaa
      721 ggtccgggtc cgcaatatcc acgatctctt caaggtcctg gaggtggaaa acatcatcct
      781 ttgcgccagt ctttcggcca ccgcttccct ggagcgtaaa gaaagccggt gggggttctg
      841 gcactatcgg gccgattttc ccaagaagga cgatgagaac tggcgcaaac acatcatgct
      901 aagcacgggg ggagatcccg aagaggtccg ggtttcccac cgccccatcg ccaggatgca
      961 gtgaggagaa aacatggatt tcaatctgct caccagcctg aatgacaata tacggatcga
     1021 tcggaataag tgcacggcct gcggacgttg cgtagaggtg tgcgttctgg ataacctgcg
     1081 gttacagctt tctccctgcc gccaggcctg ccccctgggg atgaactgcc aagggtacat
     1141 ccatcaccta gcccaaggga aaatggacaa aggactggaa aaggtccggg aggccgttcc
     1201 cttcggcggc atcctgggaa ggatttgctc ccggccctgc gaagcggtct gcaaccgggt
     1261 caaagtggac cagcagccgg tggccatccg cgatttgaaa cgctacctag ccgaccaaga
     1321 agccggggac tgggctcccc cccgcgcgcc ggagaggccg cagaaagtcg ccgtggtggg
     1381 tgccggaccg gctggattaa cggcggcctt ttacctccgt tcctggggat tccaggttac
     1441 cctctacgac agggaatcgg ctcccggggg actcatgcga tgggccatcc ctgaattccg
     1501 gctcccgtcg gaggttttca accgggaact cggtttcctt aaggatatgg ggattgattt
     1561 ccaaggaaac cggaccctgg gaaaagacct ggacctggga aaattagaag aggaatgcgg
     1621 tgccgtcctt ctggccctgg gagcttacgg acagacccgg ctgggagtgc cgggagagga
     1681 ggctcagggg gtcctgcaag ccctggattt catgaaaaac gtacgggaga agaaaccgcc
     1741 ccgggtagga ccgagggtga tcgtagtggg agggggcaat gccgccgtgg acgcggccca
     1801 gaccgccctg cgcctggggg cccggaaggt ccatctggtc agcctggaaa agagggagga
     1861 aatgcccgcc ttccgctgga gtgttgccga agccgaggaa gagggagtcg acatccaaaa
     1921 cggctgggga cccatgagat tccggatgaa tgggaagaag cttgcggggg tcagcttcaa
     1981 gaaatgcgcg gcggtctttg atccgcaagg gataatctcc cccagctacg atgagaagac
     2041 aaccctggac ctgcctgcag acacggtgat tctggcggtg ggacagaggg ctgaa
//
LOCUS       DASPRR010000600        10626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1918434, whole genome shotgun sequence.
ACCESSION   DASPRR010000600 DASPRR010000000
VERSION     DASPRR010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918434"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VEL68_22020"
     CDS             complement(<1..614)
                     /locus_tag="VEL68_22020"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38724.1"
                     /translation="MFKKVAVTAILLALILAGFFTPLSPAQAAGEVKIGLSAPITGDW
                     AEYGADFKRSVTMVFDRVNKEGGINGKKIVLEIADSRGDAKEAVLIAEKFVADPAIIA
                     QIGDFSSSCTMAAAPVYEQAKMVQISPTASHQDWTKKGEFMFRVVATQGYEGPYNARW
                     AVKDLGKKKIATVYINNDWGVDANKYFVQEAKALGAEIVAEEAFT"
     gene            complement(931..1221)
                     /locus_tag="VEL68_22025"
     CDS             complement(931..1221)
                     /locus_tag="VEL68_22025"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ38725.1"
                     /translation="MNIQLEFIGFPIIYDLFPEGPHLYALEGNTLVELIEDLIAHEDS
                     RVKEALLDPGTKSLDPTIQVMVNQKFISRAQLKEQKVEENSHVTFFKLLAGG"
     gene            complement(1331..3160)
                     /locus_tag="VEL68_22030"
     CDS             complement(1331..3160)
                     /locus_tag="VEL68_22030"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ38726.1"
                     /translation="MGRNYGRVLIVNLDDRSSRTLEIEPEILKKYVGGAGLAAYLYSQ
                     FVKDDVPPLDPRSPFFIMTGPLTGTPVVLSGRHGVAGRSPLTGFWGESSVGGHWGREL
                     RRTGYDGMILLGKADRPIYLSVQDDRLEIKDAAHLWGKDCFETDRILKKEWGDRAQVT
                     SIGLAGEKLVRFAAVNTDGPHARVSGRCGLGALMGSKKVKAILVQGTEEIPVADVQSL
                     RASLKELVPILTGKLKGLSTFGTPGLVVPCEAIGDLPVRNWGQGRWSEGAQKLSGQEL
                     SQKFLKKQFYCAGCTVGCGRTVGGVIDPALEETGGPEYETLAMLGSNCLTDDLAGVVR
                     LNELVNRLGMDTIETGAVVAFCMELAEKGLIGRAELGPLDLKWGNVQAAEGLIRMIAS
                     RQGFGDLLADGIKVAMEKIGGTALEYGIQSNNMALPAHDPRAYASLALTYATSDHGPS
                     HTESFSFWFERGSTFPEIGIEKVMDRFQSEGKAELVVKAQNVMNLWENLALCKFTVIG
                     GVQLQHISMWLKDICGWDLSVQNLIEVGERCFNLKRKLGVGWGISRKNDTLPLRVITH
                     RVDDGGCGKHLPPFNILLADYYEKRGWTKEGIPMVETYRRLGI"
     gene            complement(3276..3824)
                     /locus_tag="VEL68_22035"
     CDS             complement(3276..3824)
                     /locus_tag="VEL68_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXZ38727.1"
                     /translation="MELAGKRVGIFVEKMYQEFEFWYPYFRMKEAGAKVSIIGTGAKE
                     YPSLNGIPAPGGLAFKSVNDLVNADAVSAADFDAIIIPGGFAPDYMRRSPAVLKLVKD
                     CFEQGKIVATICHAGWVLASAEILKGKTMTCLFSIKDDVIHAGAKYVDQEVVRDGNLI
                     TSRLPDDLPAFLREIIAALKSK"
     gene            complement(3876..4583)
                     /locus_tag="VEL68_22040"
     CDS             complement(3876..4583)
                     /locus_tag="VEL68_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38728.1"
                     /translation="MMLEVQDIHTYYGQSHILQGVSLRVKEGEIVLLSGRNGAGKTTT
                     IRSIMGLVPPKSGEINFLGERIASLPVHLVSQKGISLVPEGRKIIPGLTVYENLKLAT
                     LKNKDKARVAVLLEQISQVFPRLRERWSQEGTSLSGGEQQMLAIARALVSDPVLMLID
                     EPSEGLSPIMVEKIAEVLKEIKKHGKTILLVEQNTDMALEISNRCYLMDEGVIKFEGS
                     PAEIKGNEEIQREYLAI"
     gene            complement(4580..5326)
                     /locus_tag="VEL68_22045"
     CDS             complement(4580..5326)
                     /locus_tag="VEL68_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009761280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38729.1"
                     /translation="MEILKTEKLTKYFGGLAAVLNVDFTLQKDEVQSIIGPNGAGKST
                     LFKLITGELEPTQGRIWFNDEDITGLDQHLISHKGVATSYQITNIFPKLTAFENVRLA
                     VQSRKTSYNLWSLAKSHREINEKAERILKKIGLGDKENVLAANLPYGNQRHLEIGVAL
                     GTDPVLLLLDEPTSGLSPGETRETVDLIKEIAQGLSVILVEHKMKVVMDLSDKITVLH
                     EGEVIARGNPQEIRANETVKKVYLGGVKES"
     gene            complement(5332..6228)
                     /locus_tag="VEL68_22050"
     CDS             complement(5332..6228)
                     /locus_tag="VEL68_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38730.1"
                     /translation="MPYEALATQIVIWGLLALGYNILYGYTGLLSFGHAAYFGLGAYA
                     TGIVLIRTNCSLLAGIGAGVGVACLGAMVIGFFCLRRRGIYFALLTMAFAQMLYFIAS
                     IWESLTGGEPGLRNIPHPPLEIPGLFFLNIYTPVRFYFFVFALVVISLLLLNRILESP
                     FGKVLQGIRENEKRARALGYNTNRIKWLSFILSGTFSGLAGALHTLYINFVGLEQLYW
                     ETSGKILLMNLLGGPGTFFGPFIGAGVFLYLEDIISGFTKYWMIVLGPIFVLCVLFFP
                     QGIWGTVRDLADRFKATSPKRG"
     gene            complement(6410..7297)
                     /locus_tag="VEL68_22055"
     CDS             complement(6410..7297)
                     /locus_tag="VEL68_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38731.1"
                     /translation="MFGIPGNILIFQIFTGLVLGMIFVLLAIGLSLIFGLLTVVNFAH
                     GAFYMWGAYVALLSAGYFNNFWLGLIVVPLVTGAIGMAVEYLFVRRLYGLNPDYPLLL
                     TFGLSLIMIEAIRILFGTTGYPFNAPISLQQSINFFNLFSFPLYRIFVIFVTAAVIFL
                     IWLFLEKTNVGLIIRGGTRDSEMVRVLGIDISKVWLVVFGIGTGLAGLAGILAAPMRG
                     VYPEMGIAMLVECFVVTVVGGMGSLKGAVVSGLLLGQVVSLTSLLMPEMSNIVIFIAM
                     AVVLLIRPSGLFGISGLLE"
     gene            complement(7369..8631)
                     /locus_tag="VEL68_22060"
     CDS             complement(7369..8631)
                     /locus_tag="VEL68_22060"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38732.1"
                     /translation="MRITRRNFLKSVGALGGATALGGLLPPTSFAQAKEPIKLGFIQP
                     LTGPFGTEGADQVAAATLAVDEFNAKGGVLGRKIELLVRDDKLQPEESARRAKELLEK
                     DKIHMMAGSIGAHTQLAINEQCKRAQTIFMSTSASNEITMAKDVSPYTFSEATNLHII
                     TQAVGKWTAQNLGKKWFILAANYGFGWQTTEGFRRIGKEMGCTEAGLINHPTGATDYT
                     TYFPKILAAEPEVLLLCNFGKDQLNSVKQVHEFGLKKKMKIIAPLIVITSRMAAGDEP
                     YEGMIGGATFYWGAEKTIPTAKTFVTNYRNRWGRPPTDMSGYGYSGVRGLLSAVERAG
                     AMDTKKVILALEGYTYDHYKGKQWMRAWDHRSIQDMLVLQSKSGKEKTEEWDVFKVVD
                     TVKADDKMERSAQELGLQEGVPLSKQLP"
     gene            complement(8754..10187)
                     /locus_tag="VEL68_22065"
     CDS             complement(8754..10187)
                     /locus_tag="VEL68_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXZ38733.1"
                     /translation="MAVKTVEEYVESLRKLKPTAYMFGEKLENVVDNPRIRGGINATG
                     ATYELANSEKHRDLFTTVSPFTGERINRFTLPPQSIEDLVCRVKINRTLGNHVGTCHQ
                     RCTGLDCMSALSIVTYDIDKKFGTSYYSRFLEFLKHMQRNDFTANASVTDVKGDRSLS
                     PHEQADKDLYLRIVEKQKDGIIVRGAKVHQTGSLSSHELIVLPTRAMGKGDEDYAVGF
                     AVPADTKGVIHVVGRNTLDTREIEGVDCGNSRYSKYCPTVIYDDVFVPWERVFMCGEI
                     DFSGEMVNRFSAFHRQSHGGCKSGKIDVMTGAALAMMDYNGSAKVSHLREKIVDMIYR
                     AETLYGCSLAASYEGKKEPSGTYFINSVLANASKIHEGREMAEVIRLMVDIAGGYVAD
                     LPSDRDFQNPEISGLLKKYLKGTAEAPVEHRVQMLRLVEKLALESADTVSDLHGGGSP
                     QAHRLTILRESDLNKRKNIAKRLAGIE"
BASE COUNT         2350 a         3067 c         2694 g         2515 t
ORIGIN      
        1 gtgaaggctt cctcggccac gatctccgcg cccagggcct tggcttcctg gacaaaatat
       61 ttgttggcgt caacccccca gtcgttgttg atatagaccg tggcgatctt cttcttgccc
      121 aggtccttca ccgcccaccg ggcgttatag ggcccctcgt atccctgggt ggccaccacc
      181 cggaacatga actcgccctt cttggtccaa tcctggtggg aggcggtggg ggagatctgg
      241 accatcttgg cctgctcgta gaccggcgcc gccgccatag tgcaggagct gctgaagtct
      301 ccgatctggg cgatgatggc ggggtcggcg acaaacttct cggcgatgag gacggcctct
      361 tttgcgtccc ctcggctgtc ggcgatttcc aggacgattt tcttgccgtt gatgccgcct
      421 tccttgttca cccggtcaaa gaccatggtc acggagcgct tgaaatcggc gccgtactcg
      481 gcccaatcgc cggtgatcgg ggctgaaagg ccgattttga cctctcccgc ggcctgcgcc
      541 gggctcaggg gggtgaagaa gccggccagg atcagggcca gtagaatagc tgttactgcg
      601 acctttttga acatgacgtc ctccttcttt atgggatcga atagaaaatt ggcaacgcct
      661 aaaccgggat tcagctacaa accaagaacg ctggtctggt caatggacgg tttccgtctt
      721 tctatggctt ccgccaggcg tgcagcgagg tgggtaaagg ctgagccttc ctgttcaggc
      781 cctgcattga ttttatagga actcgcctca gaataaaagg gggaagtata aggaaattgc
      841 ctggccttcc tcgcttcttc tcgggactgc ccattttgcg gccgaccatg ggggccgccc
      901 ctgaattcta atactcatac agacacgaac tcaccctcct gccagcagct tgaaaaaggt
      961 tacatgacta ttttcctcga ccttctgttc tttcaattga gctctcgaga taaatttttg
     1021 gttgaccata acctggatcg tggggtcgag agatttcgtc ccgggatcca ggagcgcctc
     1081 tttaacccgg gagtcttcgt gggcgatgag gtcctcaatt agttcaacaa gtgtgttacc
     1141 ctcaagggcg taaagatggg ggccttcggg aaatagatca tagatgatcg ggaagccgat
     1201 gaattccagt tggatgttca cgccgaatct tccaccttat aggggtgact cctcacgctt
     1261 ttcgcatgat cgtccctaca aaaagatgat cagaattaac ggggaaatcc atgaattgcc
     1321 cctgcccatt tcagattccc agtcgcctgt aggtttctac catgggaatc ccttctttgg
     1381 tccagcccct cttttcataa tagtcggcca gcaggatgtt gaatgggggg agatgttttc
     1441 cgcagccgcc gtcatccacc cggtgagtga tgactcggag ggggagcgtg tcattcttgc
     1501 ggcttatgcc ccaccccacc ccaagctttc gtttcaggtt aaagcacctc tcccccactt
     1561 cgatcaaatt ctgaacggac agatcccacc cgcagatgtc cttcagccac atgctgatat
     1621 gctgcagctg gacgcctccg atgacggtga atttgcacag tgccaggttc tcccacaggt
     1681 tcatcacgtt ttgggctttg accaccagct cggccttgcc ttcggactgg aaccggtcca
     1741 tcaccttttc aatcccgatc tcggggaagg tcgatccccg ctcaaaccag aagctgaaac
     1801 tttccgtgtg ggaagggccg tggtctgacg tcgcataagt cagggccagg ctggcatagg
     1861 cccgcgggtc atgggctggc aaggccatgt tgttcgactg gatgccgtac tccaaggccg
     1921 ttcctccgat cttttccata gctactttga ttccgtccgc cagaaggtct ccgaaccctt
     1981 gccggctggc gatcattcgg atcaggcctt cggcggcctg gacattcccc catttcaggt
     2041 ccaggggccc caactccgcc cgaccgatca ggcctttctc cgccagttcc atgcaaaagg
     2101 ccaccaccgc cccggtttca atggtgtcca tccccaggcg attcaccagt tcgttgagcc
     2161 ggaccacccc tgccaggtca tcggtgagac agttggaccc cagcatggcc agcgtttcat
     2221 actcgggccc gcccgtttct tccagagccg gatcgatcac tcccccgacg gtccggccgc
     2281 agcctaccgt gcagccggcg cagtaaaatt gcttcttcaa gaatttctgg ctcagttcct
     2341 ggccggagag tttttgggcc ccttcgctcc atctgccctg cccccagttc cgcaccggca
     2401 ggtcgccgat ggcttcgcag gggaccacca gccctggcgt tccaaaagtg ctcagcccct
     2461 taagtttccc cgtgaggatg ggcacgagtt ctttcagact ggcccgcagg gattggacat
     2521 cggcgacggg aatttcttcc gttccctgaa ccaggatcgc cttcaccttc ttcgacccca
     2581 tcagagcccc caacccgcac cggcctgaga ctcgggcgtg gggcccgtcg gtgtttacgg
     2641 cggcgaaccg gaccagtttc tccccggcta gcccgatgga agtgacctgg gccctgtccc
     2701 cccattcctt tttcaggatc cggtcagttt caaagcagtc cttgccccag aggtgggccg
     2761 catccttgat ttccagtcgg tcatcctgga ctgacaaata gatgggccga tccgctttcc
     2821 ccaggaggat catcccatca tagccggtcc gacgaagctc tcgcccccag tggcccccca
     2881 cggacgactc tccccagaat ccggtcaacg gagacctccc agcaactccg tgcctccctg
     2941 aaagcacgac cggggtcccg gtgagtggtc cggtcatgat gaaaaacggg ctccgcgggt
     3001 ccaagggagg aacatcgtct ttaacgaatt gggaatagag atatgcggcc agccccgccc
     3061 cgccgacata tttcttcaat atctcgggtt caatctccaa tgtccgcgaa cttcggtcat
     3121 ctaagttgac gatcaaaacg cgtccgtaat ttcgtcccat tccttcctcc agtcttaatt
     3181 aaatacggga aaggtcacga acgttattgt ggattccgag taggagcggg tttaaaaccc
     3241 gcccctacgg ccggacggca gggccatgaa aggttttatt tgctcttcaa ggcggcaatg
     3301 atctcccgca ggaacgcggg aagatcgtcg ggaaggcggg aggtgatcag attgccgtcc
     3361 cggacaactt cctgatccac atacttggcg cccgcatgga tcacgtcgtc cttaatcgag
     3421 aagaggcagg tcattgtttt ccctttcagg atttccgccg aagccagtac ccatccggca
     3481 tggcagatgg tggctacaat cttgccctgt tcgaaacagt cctttaccaa ctttagcacc
     3541 gccgggctgc gccgcatata atccggggca aacccgccgg ggatgatgat ggcatcaaaa
     3601 tcggcggcgc tgacggcgtc ggcattcacc aggtcgttca cacttttgaa ggccagaccc
     3661 ccgggagctg ggattccatt caggctgggg tattctttgg cccccgtgcc gatgatggaa
     3721 accttggctc ccgcctcctt catgcgaaag tagggatacc aaaattcaaa ctcctgatac
     3781 atcttctcca caaagatgcc cacccgtttc cctgccagtt ccatgttcaa acctcctttt
     3841 ttagtattcc actcacacga acactgatac ttatttcaaa tggccaggta ttccctctgg
     3901 atttcttcgt tccccttgat ctccgccggg gaaccttcaa atttgatgac cccctcatcc
     3961 attaaatagc accggttgga tatttccagg gccatgtcgg tattctgttc caccagcagg
     4021 atggtctttc catgcttttt aatctctttt aacacctcgg cgattttttc caccatgatt
     4081 ggactcagcc cctcggaagg ctcatcgatg agcattaaga ccggatcgga gaccagagcc
     4141 ctggcaatgg ccagcatctg ttgctcccct ccgctcaggc tggttccttc ctggctccat
     4201 cgttccctga ggcggggaaa gacctgagaa atctgttcca agaggactgc aactctggct
     4261 ttatctttat tcttaagcgt ggccagtttg agattttcat aaacagttaa cccggggata
     4321 atcttacgtc cttctggaac caagctgatt cctttctggg aaaccaggtg gaccggaagg
     4381 ctggcgattc tctcccctaa gaaattgatc tccccggatt tcggggggac caatcccatg
     4441 atgctccgaa tggtcgtagt ctttcccgca ccatttcgcc cggaaagaag aacaatctcc
     4501 ccttccttca cccggagcga aacgccctgc aggatgtgac tctgcccgta ataggtgtgg
     4561 atatcctgga cctccagcat catgattcct tgacccctcc gaggtatacc ttctttaccg
     4621 tttcgttggc ccgaatctct tgggggttgc cccgggcgat gacctctcct tcatggagga
     4681 cggtaatctt atccgaaagg tccatgacca ccttcatttt atgttccacc aatattaccg
     4741 agagaccctg ggcgatttcc ttgatcagat cgacggtctc tcgggtctcg cccgggctca
     4801 aacccgaagt aggctcatcc aggaggagaa ggactgggtc cgtccccaaa gcgaccccga
     4861 tctccaggtg tctctgattt ccatagggaa ggttcgcggc caggacattc tctttatccc
     4921 ccaagccaat cttctttaaa attctttccg ccttttcatt gatttcgcga tggcttttcg
     4981 ccaacgacca taggttgtag gaggtcttgc gggattggac cgctaaacgc acattctcga
     5041 aagcggtcag tttggggaaa atattggtga tttgataaga ggtcgccaca cccttgtgcg
     5101 atatgaggtg ctgatccaac ccggtaatat cttcatcgtt aaaccagatt cttccttggg
     5161 taggttctaa ttcaccagta atcagcttga aaagggtgct tttcccggcg ccgttcggac
     5221 cgataataga ttggacctcg tctttttgga gggtaaaatc tacattcaag acagcggcca
     5281 gccccccaaa atatttagtc agcttttccg tcttcaggat ttccaacggc tctatcctct
     5341 cttcggggaa gtggctttga atcgatcggc aaggtcccga accgttcccc agattccctg
     5401 aggaaagaac aaaacacaga ggacaaaaat tgggcctagg acgatcatcc agtacttggt
     5461 gaatccactg atgatatctt ccaggtagag aaaaacccct gcgccgatga agggaccgaa
     5521 aaatgtcccc ggccccccta ataaattcat caacagaatt tttcccgatg tttcccagta
     5581 aagctgctcc agccccacaa aatttatgta gagagtgtgc agcgctccag ccagtccgga
     5641 gaaggttccg gatagaataa aggaaagcca cttgatccga ttggtgttgt accccagggc
     5701 cctggctctt ttttcatttt ccctgatccc ctgcagcact tttccgaaag gggattcgag
     5761 gatccggttt aacagcagaa gggagatcac caccaaggcg aagacaaaga aataaaagcg
     5821 aaccggcgtg taaatattga ggaagaacag tcccggaatt tccaggggcg ggtgcggaat
     5881 gtttcgcaga cccggttctc ccccggttaa tgattcccag atactggcta taaagtagag
     5941 catttgggca aaggccatgg tgaggagggc aaaatagatt ccccgccggc gcagacagaa
     6001 aaaaccgatg accatggccc cgaggcaggc caccccaact cccgcaccga tccctgccag
     6061 aagagaacaa ttcgtccgga ttaagacaat cccggtggcg taggcgccca atccgaaata
     6121 ggcggcatgg ccgaaggaaa gcaagccggt gtagccgtat agaatgttat atcccaatgc
     6181 cagaagcccc cagattacga tttgagtcgc tagagcttca tagggcaaaa ggaaagggat
     6241 ccctacgagg acaaggatga tcatcaaaac cgagtgatcg gttatccacc gtaaacattt
     6301 tttccatgat gggtctggag tcatgatcga tatgggatgc taccaaattc cgttcaaatg
     6361 gagaccgtag gagccattca tgaattgccc ttacaggatg aaactggctt tattcaagca
     6421 gaccggatat cccaaacagg ccgctgggcc tgatcaaaag caccactgcc atggcaataa
     6481 aaataacgat attggacatc tcgggcatga gtaaagaagt taaactgacc acctgcccga
     6541 gaagcagccc gctcaccact gcgcccttca gactccccat cccacccacg acggtcacca
     6601 cgaaacactc tacgagcatg gcgattccca tttccggata gactccgcgc atgggtgcgg
     6661 ccaggatgcc cgccagaccc gccaagccgg tcccgattcc aaagactaca agccaaacct
     6721 tggagatatc gattcccaaa actcggacca tttcgctatc ccgggtacct ccacggatga
     6781 tcaatcccac attggtcttc tccaagaata gccatattag aaagatgacc gcggcggtga
     6841 cgaagataac gaaaattcta taaaggggga aagaaaataa attaaaaaaa tttatgcttt
     6901 gctgtaaaga aatgggggca ttgaagggat acccagttgt tccaaatagg atccgaatgg
     6961 cttcaatcat gatcaaagac aacccgaaag tcaggagcag ggggtaatcc gggtttaagc
     7021 catagaggcg acgaacgaat agatattcta cggccatgcc aatcgccccg gtgaccaggg
     7081 ggacgacgat taaccccaac cagaagttgt taaaatatcc cgcagataaa agagcgacat
     7141 aggcccccca catgtagaag gcgccatgag caaaattgac gacggtgagc aaaccgaaga
     7201 tcaaagagag cccgatggcc aacagaacaa agatcatccc caggaccagg ccagtaaaaa
     7261 tttgaaagat aagaatattc ccgggaatac caaacataat gggcgcaccc cgcttaacag
     7321 taggggcgac ccggtgggcc gcccctacaa gaccccaggc acggatggtt aggggagctg
     7381 tttgctgaga ggaacaccct cctgcaggcc aagttcctga gccgagcgtt ccatcttatc
     7441 atccgccttc accgtatcca caactttgaa aacgtcccat tcttcggttt tttctttacc
     7501 gctcttggat tggagcacta gcatgtcctg gatcgaccgg tgatcccagg ccctcatcca
     7561 ctgtttgcct ttgtaatggt cgtaggtata accttcgagg gccaggatga ctttcttcgt
     7621 gtccatggcc ccggccctct ctacggcact gagcagccct ctaaccccac tgtacccata
     7681 tcccgacata tccgtggggg gtcttcccca tcgattgcgg taattggtca caaaggtttt
     7741 ggcggtggga atggtcttct ctgcccccca atagaaggtg gctcccccaa tcattccttc
     7801 atacggctca tcgcccgccg ccatcctcga ggtaatcacg atgagcggag caatgatttt
     7861 catcttcttc ttcaagccga attcatgaac ctgcttaacc gaattgagct gatccttgcc
     7921 gaaattgcag aggagcaata cttccggttc agcggccagg atcttgggaa aataggtggt
     7981 ataatctgta gctcccgtgg ggtgattgat caggcctgcc tcagtacatc ccatctcctt
     8041 gccaatccgg cggaaacctt cggtggtctg ccacccgaat ccataattgg ccgccagaat
     8101 gaaccatttc tttcccaggt tctgcgccgt ccattttccc acggcctggg tgatgatgtg
     8161 gaggttggtc gcctcggaaa acgtgtatgg gctcacatcc ttggccatgg tgatttcatt
     8221 ggaggcgctg gtggacatga aaatggtttg agcccgttta cactgttcat taatggccag
     8281 ctgagtgtgg gcgccgatgg acccggccat catgtgaatc ttgtcctttt ccaagagttc
     8341 cttcgctcgt cgagcgcttt cttccggctg caatttgtcg tcccggacca gcagttcaat
     8401 cttccgcccc agaaccccgc ctttagcgtt gaattcatct acggctagag ttgccgctgc
     8461 cacctggtcg gcgccctcgg tcccaaaggg cccggtcagg ggctggatga aaccgagctt
     8521 gatcggctcc ttggcctgag cgaaggatgt tgggggaaga aggccgccca aggccgttgc
     8581 tcctccgagg gctcctacgg atttcaggaa atttctgcgg gtaattctca cggttctcct
     8641 cctttctttt tcatcctttc gagctatttt ctttcctttc tgtttatctc cgaaaaacag
     8701 catttggtta tggatgagat gaaaaccgca taccggggag cagccggcga accttattca
     8761 atccccgcca gccgcttggc gatattcttc ctcttgttga ggtccgactc gcgcaggatg
     8821 gtcaggcggt gggcctgggg ggagcctccg ccgtggaggt cggagaccgt gtcggcgctc
     8881 tccaaggcca gtttctccac cagccggagc atctgcaccc ggtgttccac gggagcttct
     8941 gccgttccct tcaggtattt tttcagcaac ccagagatct ccgggttttg gaaatcccgg
     9001 tccgagggca ggtcggccac atatcctccg gcgatatcca ccatgagacg gatgacttcc
     9061 gccatctctc tgccctcatg aatcttggag gcattggcca ggaccgagtt gatgaaatag
     9121 gtcccggaag gctctttctt cccctcgtag gaagcggcca ggctgcaccc gtagagggtc
     9181 tccgcccggt aaatcatatc cacgatcttc tcccggagat ggctgacctt ggccgagccg
     9241 ttgtaatcca tcatcgccag ggcggccccg gtcatgacat cgatcttccc cgatttgcac
     9301 cccccgtggg actgacggtg gaaggcggaa aaacggttca ccatttcccc ggagaaatcg
     9361 atctccccgc acatgaagac ccgctcccag ggcacgaaca cgtcatcata gattaccgtc
     9421 ggacagtact tgctgtagcg ggaattgccg caatccacgc cctcgatctc ccgggtgtcg
     9481 agcgtgttcc ttcccaccac gtgaatgacc cccttggtat ccgcagggac ggcgaaaccc
     9541 acggcatagt cctcgtctcc cttccccatg gcccgcgtgg gcaggacgat cagttcatga
     9601 gagctcaacg acccggtctg gtgcaccttt gccccccgga cgatgattcc gtctttctgc
     9661 ttctccacga tcctcaggta gaggtctttg tccgcctgct catgagggct gaggctgcgg
     9721 tctcctttga cgtcggtcac gctcgcattg gcggtgaaat cgttgcgttg catgtgcttt
     9781 aaaaattcca agaagcggct gtaataggaa gtgccgaatt tcttatcgat gtcataggtg
     9841 acgatggaaa gagcgctcat gcaatccagc cccgtgcagc gctgatgaca ggttcccaca
     9901 tgatttccca gggtgcggtt gatcttcacc ctgcaaacca ggtcctcgat ggactgaggg
     9961 ggcagggtga aacgattgat ccgctctccg gtgaagggag aaacggtggt gaagaggtcc
    10021 cggtgttttt ccgaattcgc cagttcgtag gttgccccgg tagcgttgat gcctccccgg
    10081 atccggggat tgtcgaccac attctccagc ttctccccaa acatgtaggc cgtcggcttc
    10141 aacttccgaa gactctccac gtactcctct actgttttaa ctgccataac cctcctcctt
    10201 tcgatataga aaacattggg acgcagatga acgcggataa acgcagataa ttctcttgcc
    10261 aaatgcagat agaaaacaat tttgatgatc ccgtaaaatg tcccaagaat cgtcaccccg
    10321 gcgcaagccg gggtccagaa tttattgaaa taactggatt ccgactttcg ccggaatgac
    10381 agattttgtg gaaattcggc tttaggagta catcaatttt tgcgcctttt tattttcctt
    10441 ctgaatcctg aaaatctgcg tgtatctgcg tcctataatt ccttcaaaat tccagtcctc
    10501 ttcggaaagc tttgaggttc atctccttgg ccttgggcgg gacgttggcc tcgatggctt
    10561 tttccatggc cccgaccgaa aggaccgccg tctttttgag cagtccgccc agcaggatca
    10621 ggttgg
//
LOCUS       DASPRR010000601         9149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_292089, whole genome shotgun sequence.
ACCESSION   DASPRR010000601 DASPRR010000000
VERSION     DASPRR010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292089"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1292)
                     /locus_tag="VEL68_22070"
     CDS             complement(126..1292)
                     /locus_tag="VEL68_22070"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXZ38734.1"
                     /translation="MTYKDRLSFGFHIPTKIVFGAGSLKEAVKEMDGLGMKKALVVTD
                     GTLKDSSMVKTLMEVLGERAAGLFPEAIPDSSFQVVSRGAGVFREKGADGIISIGGGS
                     SMDTAKAIGVLAKKGKEDLREFVGIGKIGERIVPHIAVPTTSGTASEVTQFATVKDHE
                     AREKRLLSDPGLIPPVAILDPLLTVSLPPQMTAATAMDAMSHGVESFHARFYEPIADA
                     LAHQAIRLIVQYLPVCLQKPDDLEARGMLAIASTLGGMAFQNALVGCVHGMAHALGGM
                     YGLHHGLANSILLPHGMRFNMPVSAPRYRQVAEAFGLDLKGKTDLQIGEMTVQAVEEF
                     SKKTGLPQKLSQVNIPRENMLEVAKLALNDPGMKSNLRRVTDPQEIVPILLAAW"
     gene            complement(1289..1546)
                     /locus_tag="VEL68_22075"
     CDS             complement(1289..1546)
                     /locus_tag="VEL68_22075"
                     /inference="COORDINATES: protein motif:HMM:NF044585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38735.1"
                     /translation="MKEKKKVQYRKIRAWVKTVDSNYTGTIYLSKEQGRISDLMNDER
                     QFISMTDVESKDFASPKAYLAINKDLIELVEVLDFDREREP"
     gene            1838..2044
                     /locus_tag="VEL68_22080"
     CDS             1838..2044
                     /locus_tag="VEL68_22080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38736.1"
                     /translation="MDSNKKTNGKLTEAEIAWLMNILTSADCKCCIWREKEEDRYQCF
                     PNGNSPCKVFADKVFYGLERSGLI"
     gene            2168..3883
                     /gene="polX"
                     /locus_tag="VEL68_22085"
     CDS             2168..3883
                     /gene="polX"
                     /locus_tag="VEL68_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HXZ38737.1"
                     /translation="MENKTIADIFTEIADILEIQGENPFRVRSYRNAARTIVDMSRSL
                     ESLVKAGEDLEEIPGIGKSMSEKIQEILSTGKCHTLEELRSRVPPGLTELLKLEGLGP
                     KKVKALYDELSVDSVDRLEKAAQAGRLRNLPGMGLKTEEKVLKSIEHYRAGMGRFKLS
                     VGFQYAEALLRHLKGVPGVKRLDPAGSFRRRRETIGDLDILAICGKGCKVMDRFAGYG
                     DVAEVLAKGDTKSSVRLKCGLQVDLRVLEEESYGAALHYFTGSKAHNVTLRERAKEKG
                     LKVSEYGVFRAKDEKRLSGAEEEDVFTAVGLPLIPPELREDRGEVQAAEKGTLPRLID
                     LSDVRGDLQMHTTASDGKNSILEMAQKAKDTGYAYIAITDHSKAVRVAGGMDEKQLAK
                     HLREIEKANRQISGLRILKGVEVDILPDGTLDLKDDILRECEVVLASVHSRFNMEEGE
                     MTRRVIKAIQNPNVKILAHPTGRLILEREPFKINLGEVIRAAVDHGVVLEINAYPDRL
                     DLKDVDARMARDLGAKLAINTDAHSALQLELMKFGVFTARRGWIEAKDVINTLPLERL
                     LKALR"
     gene            3976..5019
                     /locus_tag="VEL68_22090"
     CDS             3976..5019
                     /locus_tag="VEL68_22090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38738.1"
                     /translation="MKTWKNLMIFGLALAFILEASVPGMANGRIMVYSPWKDEIMLKF
                     GEMFQKETGIKLESINISTGEIHARVKGEKARPQADIWHSVRAELLDKAKEEGLIAPY
                     KPANAKFLLSPYAYSNEPTFTGTTMYPLVVGYNVNTLKKMGVEPPKSYEDLLNPQWKD
                     KIIMPHPAASGTGYAFLVTVIQLFRDKGETGIQSKKGWEYLVKLNQNVSQYTRSGSAP
                     AKLTATGEFPLCITFYDRVFQLVGEGYPISSTTPSPTFAEPSCTAIVAGAPNLDGAKK
                     FMEFILSKGAQELAVTLGNYSVRPDVAPPKGAVPLRDIKVFQDDYKWGSKYKKELLNE
                     FNSKVAMGKKTTQ"
     gene            5133..6782
                     /locus_tag="VEL68_22095"
     CDS             5133..6782
                     /locus_tag="VEL68_22095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXZ38739.1"
                     /translation="MSQLRREPVVLLVTFLTFGALLYFVAYPLYAVFRESVMDDAGKF
                     VGAANYINFLNSEYFRQVFYNTLFTSGVATVGAVLIGLVFAYGMTRIDLPWKWLFMIT
                     SVLPMITPPFINAFSLILLLGRNGVVNIFLEKWLGFKFVIYGYHGIIFSEILTTFPLA
                     YLVLSAALSGLDSTLEDSAQDLGARPLGVLFTVTLPLITPAILTATLMVFMNNLSAFG
                     APALLGGGISVLAVESVVQILGVLDWGMGTTLSVVLLIPSFLLFYFQNWYRSKRSYVT
                     ISGQPAHTEFRKTSWKIKGPIFAFCLLLSVIILITYVIIVVGGFSRVWGVDSSFTLIH
                     YRLVFTNTLRSITNSLVLSSIGALLATLLGLLTAYLVVRRVFWGRRLMDFLGTLPYAV
                     PGTMMGLGFVMAFNKAPLILTGTAFILVLDYCIRRMPFGLRSGVSTLKQIDVAMEEAS
                     ADLGAPWLTTFRKIVLPLMKPAFIAGITFAFIRAITELTSTIFLVTPRWRVMAVDIYN
                     MVESGALGGAAAMSTLLMAIVTLVLVIIYRATGATTSMFRM"
     gene            6850..7935
                     /locus_tag="VEL68_22100"
     CDS             6850..7935
                     /locus_tag="VEL68_22100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38740.1"
                     /translation="MQEKGISVSLQHLTKSFGKGTGRVTAVNDFSFDFPAGKLTTLLG
                     PSGCGKTTVLRCTAGFYEPEQGDIFIGGQKINHLPPYERPTGTVFQHYALFPHMTVYE
                     NVAYGLKIKKRSEKEIREKVSQGLSLLQLSGMEGRNPNQLSGGQQQRVAIARVLVTEP
                     KVLLFDEPLSNLDAKLRVYMRGEIRALQERLGITTIYVTHDQEEAMSISHSIVIMNSG
                     KIEQTGTPWETYRQPQTPFVAEFIGTTNFLKGEVTQVDKHSIKIRLAGTEVSAFPKVP
                     FTVGDKVLVVLRPEMIRFSGKGGPGTLPGRVQEVSFLGSLARYGIQMDNGDRVQVDEP
                     NPKEFRNKGSSVSLTLDEEAMHLQKIS"
     gene            8142..>9149
                     /locus_tag="VEL68_22105"
     CDS             8142..>9149
                     /locus_tag="VEL68_22105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38741.1"
                     /translation="MKIAILMVCFCLSILLPLDPAEAAPKDSVVIAQGIDATTLDPHM
                     RWDTAAINILMNIYDFLLVRSPDLKILPLLASSYKIVNDSTWELNLRQGVKFHNGEDF
                     NAAAVKFSLERLADPRNKLQQTALQIIERVEIINEYRVHIITKKPFPYLDAQLTNVGA
                     VIPPKYFQERGPSHFSLHPVGTGPYKLVRWTKDDQLEVEANSTYWRGVPRIKRAVFRP
                     IPEDTSRVAGLQTQELDVIVNIPPNLANLMNWKGRSTVAKIPSARTVFLVLENTKGGP
                     MADVRVRRAIAQAIDQDKIIKNILEGNAFKLASPLSKYQFGYDPSIKPYDYNPAQAKK
                     LL"
BASE COUNT         2027 a         2553 c         2523 g         2046 t
ORIGIN      
        1 gaccaccagg ggccgggaat gaatttttag aagagtctga atctgagggt gatggagaag
       61 ctcatcaacc gccgggaggg ctcgcaggta tctttgtcta agttcaggct ccatatcccc
      121 ccgtgtcacc atgcggcaag aaggatagga acaatctcct ggggatcggt gacccttcgg
      181 aggttggatt tcatgcccgg gtcgttcagc gccaatttgg ccacttccag catattctcc
      241 cggggaatgt tgacctggga tagcttctgc gggaggccgg tctttttgga aaattcttcc
      301 acggcttgga cggtcatctc cccgatctgc agatcggtct tccctttcag gtccaggccg
      361 aaagcttcgg ctacttgcct ataacgggga gcgctgactg gcatgttgaa ccgcattccg
      421 tggggcaaaa gaatggaatt cgccaggccg tggtgaagcc cgtacattcc ccccagggcg
      481 tgggccatgc cgtgaacgca ccccaccagt gcgttctgga atgccatccc ccccagggtc
      541 gaggcgatgg ccagcatccc ccttgcttcc aggtcatccg gtttctggag gcagacaggc
      601 aggtactgaa cgataagccg gatggcctgg tgagccaggg cgtcggcaat cggttcgtag
      661 aagcgggcat gaaaactctc aaccccgtga gacatggcat ccatggccgt ggccgcggtc
      721 atctgaggcg gaagactgac cgtgagcaaa ggatcgagga tggccacagg gggaatcaac
      781 ccggggtcgg aaagcagcct tttctccctg gcttcgtgat ccttgacggt ggcgaactgg
      841 gtaacttcgc tggccgtccc ggaagtggtg ggaacggcaa tgtggggaac gatgcgctcc
      901 ccgatcttcc cgatgcccac gaattcccgt aggtcctcct tccccttttt ggccagcacg
      961 ccgatggcct tggccgtgtc catggaggag ccgccaccga tggagatgat tccgtcagcg
     1021 cccttttctc ggaacacccc tgctccccgg ctgaccacct ggaatgagga atcgggaatg
     1081 gcttccggga agagccccgc ggccctctct ccaaggacct ccattagagt cttgaccatg
     1141 gagctgtctt tcagggtgcc atccgtgacc accagggctt ttttcatccc cagcccgtcc
     1201 atttctttga cggcttcttt gagagaaccg gccccgaaga cgatcttggt ggggatgtgg
     1261 aagccgaagc tgagccgatc cttgtaggtc atggttcacg ctccctgtcg aaatccagaa
     1321 cctccacgag ttcgatgagg tccttattga tggccaaata ggctttggga gaggcaaagt
     1381 ccttggattc cacgtcggtc atgctgatga actgcctttc atcgttcatt aaatcggaaa
     1441 tccggccttg ttctttggac aggtaaatgg tcccggtata gttggaatca accgtcttga
     1501 cccaagccct gattttccgg tactgaacct ttttcttctc cttcatttcc tttccctaca
     1561 aagtggggaa tccccctggc cggtctgtaa ctcatcataa ttaaaggaat atgctcttgt
     1621 caaggaatat gtaaaagagg gactgccttg taaacacctg tcttggatca tgggattttt
     1681 ttgaatgatg atctagccta aaattggacc tcggacgccc ggcggggatg gcccggggga
     1741 ccatgacagg aatctccctt tctgggaaaa aagattttga catttttgtc gcctgggaat
     1801 attcttttta cttgtttgcg cccatgggag gtttagagtg gattccaata agaagaccaa
     1861 cggaaaactc accgaggcgg agattgcctg gttgatgaat attttaactt cggcggactg
     1921 caagtgctgc atctggcgtg agaaggaaga ggaccggtac cagtgcttcc ccaatggaaa
     1981 ttccccctgt aaggtctttg ccgacaaagt attctatgga ttggagaggt cgggcttgat
     2041 ttaagggata tccgcgcatc ccccctctcc ggtttttcta tcccccacga ttccttccca
     2101 aatccccttt tcgccgtcga attcccgggg tataatctct catcccttct tccctgcgag
     2161 gtctgatatg gaaaacaaaa ccatcgcaga catcttcacg gaaatcgctg acatattgga
     2221 aattcagggg gaaaaccctt tcagggtccg ttcctaccga aacgccgccc ggaccatcgt
     2281 ggatatgagc cggagtctgg agtccctggt caaagctggg gaagacctgg aagaaattcc
     2341 ggggatcggc aagtcgatga gcgaaaagat tcaggagatt ctatccaccg ggaaatgtca
     2401 taccctcgaa gaactccgct cccgggtccc cccgggcctg accgagctct tgaagctgga
     2461 aggattgggc cctaaaaagg tcaaagcgct ctacgacgaa ctcagcgtgg attccgtcga
     2521 ccgtttggag aaggccgccc aggccggacg cctgcggaat ctcccgggca tgggtttaaa
     2581 gaccgaagag aaggtcctga aatccatcga gcactaccgg gcgggcatgg gccgcttcaa
     2641 actttccgtg ggattccaat atgcggaggc actgctccgc cacctgaaag gggtccccgg
     2701 ggtcaagcgt ctcgacccgg cgggaagctt ccgccgacgg agggaaacca tcggcgacct
     2761 ggacattctg gccatctgcg gtaaaggctg caaggtcatg gaccggtttg ccgggtacgg
     2821 ggatgtggcc gaggtgctgg ccaagggtga taccaaatcc agtgtgaggt tgaaatgcgg
     2881 ccttcaggtg gaccttcggg tcttggagga agaaagctac ggggcggccc tgcactattt
     2941 caccgggtcc aaggcccaca acgtgaccct tcgggagcgg gccaaggaaa aggggctgaa
     3001 ggtaagcgag tacggggtct tccgagccaa agacgagaag cggctgagcg gagccgaaga
     3061 agaagacgta ttcacggccg tggggcttcc cctaattccc ccggaactta gggaggaccg
     3121 gggggaggtc caggccgcgg aaaaaggaac tcttcctcgc ctgatcgacc tctcggatgt
     3181 ccgcggcgac cttcaaatgc acaccaccgc ttccgatggg aagaactcca tcctggagat
     3241 ggcccaaaaa gccaaggaca cggggtatgc ctacatcgcc atcaccgacc attccaaggc
     3301 ggtccgcgtg gccggaggca tggacgaaaa gcagttggcc aaacatctga gggagatcga
     3361 aaaggcgaac cgccagatat ctggattgcg gattctgaaa ggggtcgaag tcgacatcct
     3421 gcccgacggt accttggacc tgaaggatga tattttgcga gagtgcgagg tggtcctggc
     3481 ctcggtccac tcccgcttta atatggagga aggagagatg acccgccggg tcatcaaagc
     3541 catccagaat ccgaacgtga agatcctggc ccatcccacg ggacgattga tcctggaaag
     3601 ggagcccttc aagatcaacc tgggggaggt catccgggcc gccgtggacc atggggtcgt
     3661 gctggagatc aacgcctacc cagaccgcct ggacttgaag gacgtggatg cccggatggc
     3721 cagggacctg ggggccaagc tggccatcaa cacagacgcc cacagcgctc tccaattgga
     3781 gttgatgaaa ttcggagtct ttaccgcccg ccggggatgg atcgaagcga aggatgtaat
     3841 caataccctg ccgctggaaa gactcttaaa ggcgctgagg tagatcgggg acggggaata
     3901 atacatgacg cggggaggcg cggacacgga gatgttccgt gtcttcttgt catttagata
     3961 caagggggat tgggaatgaa gacttggaag aatctgatga tctttgggtt ggcattggca
     4021 ttcattctag aagcgtcagt gcccgggatg gccaacggaa gaatcatggt ctactctccc
     4081 tggaaagacg aaatcatgct gaagttcggg gagatgtttc agaaagagac gggtatcaag
     4141 ctggaatcca ttaatatttc cacgggggaa atccatgccc gggtgaaagg ggagaaggct
     4201 cgcccccagg cagatatctg gcattctgtc cgggccgaac tcctggataa agccaaggag
     4261 gaaggtttga ttgcccccta taagccagcc aacgccaaat ttcttctttc cccctacgcc
     4321 tattccaacg agcccacctt caccggaacg accatgtacc ccctggtagt ggggtacaac
     4381 gtgaacacct tgaagaaaat gggggttgag ccccccaaga gctatgagga cctcctgaac
     4441 cctcagtgga aggataagat cattatgccc catccggcgg caagcggcac cggttacgct
     4501 ttcttggtca cggtgatcca gcttttccgc gacaaagggg aaacaggaat tcaatccaag
     4561 aagggctggg agtatctcgt caagctgaac cagaatgttt cccagtacac ccgaagcgga
     4621 agcgctcccg caaagctcac agccaccgga gagttccccc tctgcatcac cttctatgac
     4681 cgggtgttcc agttggtcgg tgaggggtat cccatttcct ccaccacacc cagccctact
     4741 tttgccgaac cgtcctgcac agccattgta gctggggccc cgaacctgga cggggccaag
     4801 aaattcatgg aattcatcct atccaagggc gcccaggagc tggcggtaac tttggggaac
     4861 tattccgtcc gccccgacgt ggctcccccc aaaggcgccg ttccgctaag ggacatcaag
     4921 gtcttccagg acgattacaa atgggggtcc aagtacaaga aggaactcct caacgagttc
     4981 aattccaagg tggccatggg caaaaagaca acccagtaga cgcggagaca cgcggaaagg
     5041 aaaatcgttt cttaaccgcg tcctcgcgtc cccttgtcat ctaaacagct tgaggcactt
     5101 aaggcacttg agcgcatttt aggcactttt taatgtccca gctgcggaga gaacccgtcg
     5161 tccttcttgt cactttcctg accttcgggg ccctgctcta cttcgtcgcc taccccctct
     5221 atgctgtctt ccgcgagagc gtcatggatg acgcggggaa atttgtcgga gcggccaact
     5281 acataaactt cctgaatagc gaatatttcc ggcaggtctt ctacaacacc ctcttcacct
     5341 ccggggtagc cacggtaggg gcggttctga tcgggctggt ctttgcttat ggaatgaccc
     5401 gcattgacct tccctggaaa tggctcttca tgatcacctc cgtcctgccc atgatcaccc
     5461 ctcctttcat caacgccttc tccctcattc ttctcctggg aagaaacgga gtggtcaaca
     5521 ttttcctgga aaaatggctg gggttcaagt tcgtgatcta cggctaccac ggtatcatct
     5581 tttcagaaat tctcaccacc ttccccctgg cttacctggt cctctccgcg gccttaagcg
     5641 gtctggacag cacattagaa gattcggccc aggaccttgg cgccaggccc ctgggggttc
     5701 ttttcacggt gacccttcct ttgatcaccc cggccatcct gaccgccacc ctgatggtct
     5761 tcatgaataa cctcagcgcc tttggggcgc cggccttgtt gggcgggggg atttcggtgc
     5821 tggccgtgga atccgtggtc cagattttgg gggttttgga ttgggggatg ggaacgaccc
     5881 tgagtgtggt cctgctgatt ccctctttcc tccttttcta cttccagaac tggtaccgtt
     5941 ccaagcgatc ctacgtgacc attagcggac agccagccca taccgagttt cgcaagacat
     6001 cctggaagat caaggggccc attttcgcct tctgtcttct cctgtcggtg atcattctga
     6061 ttacttacgt gatcatcgtt gtcggagggt tctccagggt ctggggggtg gacagttctt
     6121 ttaccctgat tcattaccgg ctcgtgttca ccaataccct gcggtcgatt accaacagcc
     6181 tggtcctctc ctccataggt gctctcctgg caacccttct ggggttgttg actgcttacc
     6241 tggtggtgcg ccgggttttc tgggggcgga ggcttatgga ttttctgggg accctcccct
     6301 atgccgtccc cggtaccatg atggggctgg ggtttgtgat ggccttcaac aaggctcccc
     6361 tcatattgac cggcaccgct tttatccttg ttctggatta ctgcattcgt cggatgcctt
     6421 tcggcctccg ctccggggtc tccaccctca agcagatcga tgtggccatg gaagaggcct
     6481 cggccgacct aggcgccccc tggctgacta ccttccggaa gatcgttttg ccgctgatga
     6541 agccggcctt catcgccggg atcaccttcg ctttcatccg ggccatcacc gagttgacct
     6601 cgacgatttt ccttgtgact ccccgatgga gggtgatggc cgtggacatc tataacatgg
     6661 tggaatccgg ggccttgggc ggggcggcgg ccatgtccac gctgctcatg gccatcgtca
     6721 ccctcgtttt ggtgatcatt taccgggcca caggagctac gacatctatg ttccggatgt
     6781 aaagactttt tgggcagaaa tgtagggtgc gttccccgaa cgcaccgttt atagtctagg
     6841 aatttgccta tgcaagaaaa gggaatctca gtttctctcc agcacctgac caagagtttc
     6901 ggaaaaggga ccggccgggt gacggcagtc aatgatttca gctttgactt tccggccggg
     6961 aagcttacca ccctcctagg ccccagcggc tgcgggaaga ctacggtctt gaggtgcact
     7021 gccggtttct acgagccgga gcagggagac atctttatcg gggggcagaa aataaatcat
     7081 ctgccgccat atgagcgccc caccggaacg gtctttcagc actatgctct ctttccccac
     7141 atgaccgtct atgaaaacgt ggcttatggc ctcaagatca aaaaaaggtc ggagaaggaa
     7201 attcgggaaa aagtctctca aggtctatct cttctccagt tgtcgggtat ggaaggccgg
     7261 aaccccaatc agctcagcgg gggccaacaa cagcgggtgg ccatcgcccg ggttctggtc
     7321 accgaaccca aggttctgct cttcgacgaa cccctgagca acctggacgc caagcttcgg
     7381 gtgtatatgc gcggggaaat ccgtgctctt caggagaggc tgggaattac caccatttac
     7441 gtcacccacg accaggaaga agccatgtcc atctcccact ccatcgtgat catgaactcc
     7501 ggcaagattg agcaaaccgg aaccccctgg gagacctacc gccagcctca gacccctttt
     7561 gtcgccgaat tcatcggaac gacgaatttc ttgaaaggag aagtcactca ggtcgataaa
     7621 cattcgataa aaatcagact ggcaggaacg gaggtttctg cattcccaaa ggtccccttc
     7681 acagtcgggg ataaggttct ggtggtcctt cggccggaga tgatcaggtt ttcgggaaaa
     7741 ggggggccgg ggactctgcc cggcagggta caagaagtct cttttctggg ctctctggcc
     7801 cgttatggga tccaaatgga caatggagac cgggtccagg tggacgaacc gaatcccaaa
     7861 gagttccgaa acaaggggtc ctccgtttcc ctcactctgg atgaagaagc catgcattta
     7921 caaaaaatat cgtaggaggc ggatctacta aggcgtcctt aattgagagg acctattctt
     7981 ccgaaaatct cctctaatcc cgatgttttc tctcgggctg tcaccgttca gcttcgacag
     8041 gctcagcacg aacggcttac tcttcagcgt ccatacaatt atggcctgat tagtaagcca
     8101 gcgattcggg aaaaggccgg agattttcta aggaggcgtt catgaagata gccattttga
     8161 tggtttgttt ttgtctatcc atccttctcc ctcttgatcc cgcagaagct gcccccaagg
     8221 actcggtggt cattgctcag gggatcgatg ccaccactct cgatccccat atgcggtggg
     8281 atactgcggc gataaatatc cttatgaaca tctatgattt tctcctggtc cggtctccgg
     8341 acctcaagat actgcccctt ttggccagct cttacaagat cgtcaacgac agcacttggg
     8401 aattgaacct gcgccaggga gtgaaattcc acaatggaga ggatttcaac gccgccgcgg
     8461 tcaagttcag cctagagcgt ttagcggacc ccaggaacaa gctgcagcaa accgccctgc
     8521 agattatcga aagggtggaa atcattaacg agtacagggt ccacataatc accaaaaaac
     8581 cctttcctta cctggatgcc cagttgacca acgtgggcgc ggtgattcca cccaaatatt
     8641 tccaggaaag gggcccgtcc catttctccc tgcatcccgt gggcaccgga ccttacaagt
     8701 tggtccgctg gaccaaagat gatcagcttg aggtggaggc caattcgacc tactggcggg
     8761 gagtcccacg gatcaagagg gccgtcttcc gccccatccc ggaagacacc tcgcgggtag
     8821 ctgggctgca aacccaggaa cttgacgtga tcgtgaacat cccccccaac ctggcgaacc
     8881 tgatgaactg gaagggccgc tccacggtgg ctaaaattcc cagcgctcgc acggtctttc
     8941 tggttttgga gaataccaag ggtggcccca tggccgatgt ccgggttcga agggccatcg
     9001 cccaggctat cgatcaggac aagatcatca agaacatcct tgagggaaat gccttcaagc
     9061 tggcttcccc cttgtccaag taccaattcg gctacgaccc ctcgatcaaa ccgtatgatt
     9121 acaacccggc tcaagccaag aagctcctg
//
LOCUS       DASPRR010000602         9960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2243861, whole genome shotgun sequence.
ACCESSION   DASPRR010000602 DASPRR010000000
VERSION     DASPRR010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243861"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1245
                     /locus_tag="VEL68_22110"
     CDS             310..1245
                     /locus_tag="VEL68_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXZ38742.1"
                     /translation="MNAPAWFVGIDVCKGTLDVFVRPTGEAKTFVHTEEGLAQMADWL
                     QSFPQALIVLEATGGWEMGAVRMLVSRGLFPVIMNPRQIRDFAKATGILAKTDKIDPQ
                     VIARFAEAVRPEIRPLATPEAQELEALNVRRRQIVEMLTTEKNRLGLAPRWVRKEIQA
                     HIRWLEKCLEKVNKELKDRIQKSPLWREKDEILRSAQGVGPVTSTTLLCDLPELGTLN
                     RKQISALVGVAPLNRDSGLFRGRRMIWGGRAAVRSALYMSTLVAIRFNPVIGAFYERL
                     RAAGKPFKVAVTACMRKLLTILNAMIKNRTRWQAV"
     gene            complement(1441..2721)
                     /locus_tag="VEL68_22115"
     CDS             complement(1441..2721)
                     /locus_tag="VEL68_22115"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ38743.1"
                     /translation="MLSLAVLGVIFLLFLVMGIPITFSLGLSSAAGLLLMDRPLSTVV
                     ISIFQGIESWVLLAIPSFIFAGILMERCGVSYRLIDFARSMVGWIRGGLGMTTVVGEI
                     MFSGVSGSTVADVSAIGSMMAPPMLKAGYKPEHTVSIIAAATCFGVLIPPAIFMIVVG
                     TQTATSVVGIFLGAILPGLVAGILLMVTIYIQAIRFKWPVDARPNLKWFFDSFKRALI
                     ALFVPIVILGGFRYGIFTATEAGAICAFYAVVVGLFIYRNVTLKEILDIGMETALLTA
                     AVVFLLGTATVYQYIMASLGVPRLFLVLLQDLSPLNFLLVTSLIILLFGLVMEGLPAA
                     VIVLPVIFPVVVAKGIHPIHFCVVITLAVMIGFFLPPTGAGLLLCLRLTNQKLTWPFM
                     RSYIPYVLTGVVSLIIIILFPSLSLIFPFHAGIR"
     gene            complement(2799..3308)
                     /locus_tag="VEL68_22120"
     CDS             complement(2799..3308)
                     /locus_tag="VEL68_22120"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ38744.1"
                     /translation="MRKDQKSPGKVYRVLSQIPEQILLIILIVTMLDMVLSVFTRYVT
                     GKAIYWAEEVGTFGLAWMTMIGTGIAVKKGIHFAMPTFIGHFPPGVRYAIALANHALI
                     TAFGVLMLLTGTNMTIASSNMFSPALELNLGILNSAAIACGVLIIVYEVQRGIGTIRT
                     GAAPFPGGH"
     gene            complement(3593..4585)
                     /locus_tag="VEL68_22125"
     CDS             complement(3593..4585)
                     /locus_tag="VEL68_22125"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ38745.1"
                     /translation="MKGRSIFIIGLVAGLILIGFPGASQAKRVLDLGDPIGGPPGEAP
                     LRVAMEFFSAEIPKRTNGEIVINYHPQTIVTSETKAIEMCKVGALAFAGTGGGLGTIF
                     PNTQVLALPYLLRDYAHYYSVVRGPIGKEMEAEIEKKHGLKVIIWLEFGFRQFANTKR
                     PLNTLEDFKGLKLRVMPSPPLADMVNALGASAVPISWAEAIPAVQQGVVDGLDLPVAN
                     LISMKIYEICKYAAMSNHYFGPQAFVANLGIWKSLTKQQQDVILKTGLEAADINWSMM
                     EKMDRDGKNQLEKRGMQVTTPNLAPFKEVAREKVYPKYYSTFGKEIIQRIMDFK"
     gene            5211..5495
                     /locus_tag="VEL68_22130"
     CDS             5211..5495
                     /locus_tag="VEL68_22130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38746.1"
                     /translation="MSSKVVLLVPEGRVRVLPITPPPRMSELRGKVVGFLHNGKPNAD
                     ILLARLADLLQERHGLAQARTRSKPRVTEPAEFIEALASECQGVVNALGD"
     gene            5619..6929
                     /locus_tag="VEL68_22135"
     CDS             5619..6929
                     /locus_tag="VEL68_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38747.1"
                     /translation="MATLPLVVVPHPVGGLRRGEVEKKADQVLEDVVRALMEGGQAVG
                     DGEKKGRTRNQPLKAKTLFAPSTADSSGPEEEERVMVADNPETIFQLFMARGWMDGLP
                     FIPPKEDRVARMLAYTDRDPKEVVALLPPRWGEATVEKIAVNAVMAGCLPQYFPVVMT
                     AVSAMAEEPFNLYAVQATTHPCSPLILVNGPLAKELNINGRYNALGQGWRANATIGRA
                     VRLILLNIGGGEPGVLDRATVGQPGKYSYCLAENEAENPWMPLHVEKGFSKEDSTVTV
                     FGAEAPHNLNDHRSTTAKDILKTFTHSIAIPGCNNALTAGEFLLLLGPEHAATIAGDG
                     FSKEDVKKFLYEEARVPVEHFPAVSWPWIRQMRPQWAEVIDKKGKIPLVDRWEEIQVA
                     VAGGAGKHSAFIPTFGATKPVTKAVVRKDGRPIRSIKEFKEKKG"
     gene            6971..7261
                     /locus_tag="VEL68_22140"
     CDS             6971..7261
                     /locus_tag="VEL68_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HXZ38748.1"
                     /translation="MAAYLVTEIEVTDPAGYEEYRKGVGATLAAYGGRNLVRPGGKIE
                     VLEGDWVPKRLVILEFPSIAKLKAWYESPEYQPLLKIRLRTAKSKMVLIEGA"
     gene            complement(7385..8698)
                     /locus_tag="VEL68_22145"
     CDS             complement(7385..8698)
                     /locus_tag="VEL68_22145"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ38749.1"
                     /translation="MSDFLVGTILIGLLFFVLSIGLEVALSLGLVGILGLLWLKGWVV
                     GMSAIGSIAWSTASNFNYIALPLFIFMSAILLHSGIGSSLYTAVTRWVSFLPGGLAVA
                     SVFACAIFGAVCGSSVATAATIGLIAIPEMEKRGYQKELMYGSMAAGGTLGILIPPSI
                     PMIIYGVMTETSVGQLYVAGIIPGILLSIFFAGYIIVRCALHPELAPKVTERVYWRDR
                     FYSLAEAAPMVGLIFIVLGTMYFGVVTPTEAAALGAFASMILAAGYRRLSWNTLVNAF
                     RDTIGPTSMCLLIITFASIFSHVVALLGAPKAIFQLIQDLGLPPWGVFALIFLLLILI
                     AYALEELSVMIIMLPFLFPLVTGLGFDGIWFGIVMVVWLEMGFITPPVGINLFVIQGV
                     AKGSTMKEIAWGSTPYVIMMIIFVGILFFFPDLALWLPRQMTPGR"
     gene            complement(8695..9228)
                     /locus_tag="VEL68_22150"
     CDS             complement(8695..9228)
                     /locus_tag="VEL68_22150"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXZ38750.1"
                     /translation="MDSKKRNVLERFSEWIILLTGLAVLAMTLMVSYDVLMRYFLGEP
                     QLFVDDLTSFLLVAMIFLGAGPVFYRGGHIRIDLVTSHLKSKTQRRMRVITLFMGIAF
                     LIIVIYETMVSTVSALQTGRVSAVMNYPLWVGMIFIPLGLILMVFFMGVQLVNEMKGK
                     VEKGREDAKDISMEISH"
     gene            complement(9408..>9960)
                     /gene="dctP"
                     /locus_tag="VEL68_22155"
     CDS             complement(9408..>9960)
                     /gene="dctP"
                     /locus_tag="VEL68_22155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXZ38751.1"
                     /translation="STKKPFAKVEDWKGAKIRVYGSETAELTKALGGAPVNIPFGEVY
                     TALQRNVADGAITSATNAEPMKFFEVTKFINYWFISGAGIEFLGCNKKALNALPPDLQ
                     QVVLDAVKESRFEDKEWEEAKAWDARAKKRCAELGLTIVDVSPAEIEKARKMVKPAWD
                     NWLKRTGAEGQRALDLAAKALGR"
BASE COUNT         2358 a         2763 c         2869 g         1970 t
ORIGIN      
        1 cccccttgta tattacccaa gggagttatc catagtcccg gagcgacgcg cccccgggag
       61 caggtgagcg aaggggctgt ggataactcc cttgaggtaa ataaaaaaaa tgtaccgaac
      121 gcagagatat cttgtgatac ttgattctga gctgccgtgc tggcttcggg gcagaccgtt
      181 gccccctggg gtcctgaacc cgtgggtgag cctgggtcgt cccgaagccc tctcccgaat
      241 agcccggaca gttgcttggg acctttggga tcccgcatct tcgcaaggaa gggtaaaagg
      301 aggatatcta tgaatgcccc agcatggttt gtcggaatcg atgtatgcaa agggacgctg
      361 gatgtttttg tgcgtcctac cggagaagca aagacttttg tccacacgga agaaggctta
      421 gcccaaatgg ccgattggct ccaatcgttt ccccaggcct taatcgttct ggaggcgacg
      481 gggggctggg aaatgggggc cgtgcgcatg ctcgtttctc gaggcctgtt cccggtgatt
      541 atgaaccccc gccaaatccg ggattttgcc aaggccaccg gaatcctggc caaaacggac
      601 aagatcgatc cccaggtcat cgcccgtttc gccgaagcgg ttcggcccga gattcgaccc
      661 ctggccacgc ccgaagcgca ggaattggaa gctctgaatg tgcggcgacg ccaaatcgtg
      721 gagatgttga ccaccgaaaa gaaccgtctc ggcctggccc cccgctgggt ccgcaaggag
      781 atccaagccc acattcgatg gttggagaaa tgtttggaga aggtgaataa ggaactgaag
      841 gatcggatcc aaaagagtcc tctgtggcgg gagaaggacg aaattttacg cagtgcccaa
      901 ggagtggggc cagtgacctc tacgacgctg ctttgcgatt tgcccgagtt aggaaccttg
      961 aatcggaagc agatttcggc tttagtaggg gtggccccgc tgaatcgaga cagcggtttg
     1021 tttcggggcc ggcggatgat ctggggaggt cgagctgcgg tgcgaagtgc gttatacatg
     1081 agtaccttag tggccattcg ttttaatccg gtcattggag ccttttatga gcgtttgcga
     1141 gcggctggga aaccctttaa agttgcggtc acagcatgca tgaggaagct cctgaccata
     1201 ttaaatgcca tgataaaaaa tcgcacccgg tggcaggccg tttaaacatc tttcacttga
     1261 cataaaagac agttgctcac cccaaccctc tcccgcaagg ggagaggggt tttcagattg
     1321 acactggcca aggcagagcc caaccgattt ttttctattt tctaacgtag tgggggcaat
     1381 tcatgaattg cccctatgcc atccatcgcc tttttcttta acccggcgag gggaaaatcc
     1441 tcatcgaatc ccggcgtgaa aaggaaagat caaactgagg ctggggaaga gaataataat
     1501 gatgagcgag acgacgccgg ttaacacata aggaatatag ctgcgcatga atggccaggt
     1561 aagcttctga ttcgtcagcc gcagacacaa caacagtcca gcccccgtgg gcggaaggaa
     1621 aaaaccgatc atgacagcta gcgtaataac gacacaaaaa tggatcgggt gaatcccctt
     1681 ggccaccaca accggaaaga tgaccggcag aacgatgacc gccgcgggca gcccttccat
     1741 gaccagaccg aagagcagaa tgatcaacga ggtcaccaaa agaaagttta agggagacaa
     1801 gtcctgcagg aggaccaaaa agagccgggg cacacccaaa ctggccataa tgtactggta
     1861 aacagtggcc gtgcccaaga gaaaaaccac cgccgccgtg agcaaggcgg tttccatgcc
     1921 gatgtcgagg atttccttca gggtcacgtt gcggtagatg aataaaccca ccacgacggc
     1981 gtagaaagcg cagatcgccc ccgcttccgt ggccgtgaat atcccatacc ggaaaccgcc
     2041 caggatcaca atgggaacga agagggcaat cagcgctctc ttgaatgaat cgaagaacca
     2101 tttcagattc ggtctcgcat ccaccggcca tttgaaccgg atggcctgga tgtagatggt
     2161 gaccatcagc aggatcccgg ccaccaggcc cggcaggatg gctcccagga agatgccgac
     2221 taccgatgtg gctgtctggg ttccgacgac gatcatgaag atcgccggcg gaatcaagac
     2281 cccgaaacat gtcgccgcag cgatgatgga aaccgtatgt tccggcttgt acccggcctt
     2341 cagcataggg ggggccatca tcgagccgat ggcggacaca tcggcgacag tggagccgga
     2401 gaccccggaa aacatgattt caccgaccac cgtggtcatc cccaggcctc cccggatcca
     2461 tcccaccatg gaccgggcga aatcgatgag gcgatagctg acgccacacc gttccatgag
     2521 aataccggca aaaataaagg aagggatggc cagcaggacc caggattcga tgccctgaaa
     2581 gatggaaatg accaccgtgg ataagggacg gtccatgagc agcaagccgg cggcgctgga
     2641 caggcccaag gaaaaggtga tcgggatccc catgaccagg aataaaagaa aaatgacacc
     2701 caggaccgcc agactcagca tatcttctta tccctttcta taaactataa acgcttgccc
     2761 ccgccgggga agatcaccgc ttccgccccc ggcccaaact agtgccctcc cggaaatgga
     2821 gcggcccccg tccggatggt gccgattccc cgctgcactt cgtagactat gatcagcacc
     2881 ccgcaggcaa tcgccgcact gttgagaatt cccaggttca gctcgagggc cggggagaac
     2941 atgttggagg atgctatggt catgttggtt cccgtcagga gcatcaaaac gccgaaggcg
     3001 gtgatcaggg catggttggc cagggcgatg gcgtaccgga cccccggagg gaaatggccg
     3061 atgaaggtgg gcatcgcgaa atggattccc tttttgaccg cgatccctgt cccgatcatg
     3121 gtcatccacg ccagcccgaa agtgcccact tcttcggccc aataaatggc ctttccggtc
     3181 acgtaacggg tgaacacgct gagaaccata tccagcatgg tcacgatcag tataatcagc
     3241 aggatctgtt ctgggatttg ggacaggacc cggtacactt tcccggggct cttctgatct
     3301 ttcctcatcg ccggggaatt ctccaatttt accttctcct cttttccttg gtgggaagac
     3361 ccccggtgcg ttaaagcttg accacgtata tctatacaca gcgacattaa tccctgcaaa
     3421 gatggtcctt cccgcgaaag cgggaatcca ggaaaaaact ggattccggg tcaagcccgg
     3481 aatgacgaag gctataagat ttaggtcgtc gtgtttagtt accggtttct tgtctaacag
     3541 cggatgcctt agcgcctccg ggggcagatt gggtggccgc gatcctcagc ggctatttaa
     3601 aatccattat gcgctgaatg atctccttgc cgaaggtgga atagtacttc gggtatacct
     3661 tttccctggc gacttccttg aagggcgcga ggttcggcgt ggtcacctgc attccccgct
     3721 tctccagctg gttcttgccg tcccggtcca tcttttccat catggaccaa tttatgtcag
     3781 ccgcctcgag ccccgtcttc aggatgacgt cctgctgctg cttggtcagg cttttccaga
     3841 tgccgaggtt ggctacgaac gcctggggac cgaaatagtg gttggacatg gcggcatatt
     3901 tgcagatctc gtagatcttc atcgagatca ggttggccac aggcaggtcc agcccgtcga
     3961 ctactccctg ttgcaccgca ggtatagcct ccgcccagct gatgggaacc gcgctggctc
     4021 ccagggcgtt gaccatgtcg gcgagcggtg gggagggcat gacccttagc ttgaggccct
     4081 tgaaatcttc aagggtgttc aggggcctct tggtattggc gaattgccgg aagccgaact
     4141 ccagccagat gatgaccttg aggccgtgct tcttctcgat ctcggcctcc atctctttcc
     4201 cgatgggtcc ccggacaacg ctgtaataat gggcgtagtc gcggagcagg tagggcagcg
     4261 ccaggacttg cgtgttgggg aaaatagtcc cgagaccccc gccggtcccc gcaaaggcca
     4321 gggcgcccac cttgcacatt tctatggcct tggtttcgct ggtcacgatc gtctggggat
     4381 ggtagttgat cacgatctcc ccgttggtcc tcttggggat ttcggccgag aagaattcca
     4441 tggccacccg cagcggagcc tcgccgggag gcccgccgat gggatcacct agatccagaa
     4501 ccctcttggc ctgcgatgcg ccgggaaagc cgataaggat cagcccggcg accagcccga
     4561 tgatgaaaat gcttcttcct ttcattttgc ctcctccttt tctcttacag ggatcgattc
     4621 ctattccctt cgtttccctc ttttattcat tcccaagcga ttccttaagg cgccggagcg
     4681 atttgataga gccatttccc gtttggagaa ctggtgaaac ttaggcctga aatggatcat
     4741 accataggcg aaacatgcgg gagtatatca aaaaacaggg gatattcccg aagtcgcggt
     4801 aacggaatct aaaaggaaag aaaaaccgat ttgccaatgt cagtatcgca acgattcccg
     4861 ggaggtctcc catgaaaacc gagaataaag ggcccgggtc ggccaagacc taaacgggga
     4921 gaccaaaact cccacccctc caaccggtga gaagaaaaat attaactcag cgccgatcgg
     4981 aaaaagaatc taaatttttg aaacccggcg ggatttattg ttctgcatga atactaaata
     5041 gtccttttct tgtcaaggaa aaaatgggaa aatgacaccc attgtgactc actccatccg
     5101 agaatttcat ttacccttca gcaaagatgt ggccccattt tggccctcgg ttcgttcggc
     5161 ggcctaatcg gaatacggat aagcttcatg ggtaagaaag gggaaaccgg atgagttcga
     5221 aagtcgtgtt gctggtgccg gaagggcggg ttagggtttt gcccattact ccgcccccgc
     5281 ggatgagtga actgaggggg aaagtcgtcg ggttcctgca taacgggaaa ccgaatgcgg
     5341 atatcctcct cgcccgcctg gccgatctcc tgcaggaaag gcacggcctt gcccaagccc
     5401 gaacccgatc caagccgcgg gtaaccgaac cggcggaatt cattgaagcg ctggcgagcg
     5461 aatgccaggg ggtagtgaac gccttagggg actgagggtc gtgcacttcg tggagtatcc
     5521 acgatgcgat tgagctggaa aagcgcggaa ttcccacggt aaccatatgc accgatgaat
     5581 tcctggccct gggtctggcc gaagccaaag ccctgggcat ggccaccctt cccctcgtgg
     5641 tcgtccccca tccggtgggg ggcctacgcc ggggagaggt ggagaagaaa gcggaccagg
     5701 tcttggaaga cgtggtccga gctttgatgg aggggggaca agcggtcggc gatggagaaa
     5761 aaaagggccg cacgcggaac cagcccctca aggccaaaac cctctttgcc ccctcgacgg
     5821 ccgactcgtc aggccccgag gaggaggaac gggtgatggt cgccgataat ccggagacca
     5881 ttttccagct ctttatggcc agagggtgga tggacggcct gccttttatt cctcccaagg
     5941 aggaccgggt agccaggatg ctggcttaca cggaccgaga ccccaaggag gtcgtggccc
     6001 ttcttcctcc ccgctgggga gaagcgacgg tcgaaaagat cgctgtcaac gccgtcatgg
     6061 ctggctgcct gccgcagtat ttccccgtgg tcatgaccgc cgtctccgcc atggccgagg
     6121 aacccttcaa cctttacgcc gtccaggcga caacccatcc ctgctctccg ctgattttgg
     6181 tcaacgggcc cctggccaag gagctgaaca tcaacggccg gtacaatgcc ctggggcagg
     6241 gctggagggc caacgccacc atcggccggg ccgtccggct gatcctcctg aatatcgggg
     6301 gcggagagcc cggcgtcctg gaccgggcga ccgtgggtca gccgggcaaa tactcctatt
     6361 gcctggcgga aaatgaagcc gaaaacccat ggatgcccct gcatgtggaa aaaggatttt
     6421 ccaaagagga cagcacggtg accgttttcg gcgcggaggc tccccacaat cttaacgacc
     6481 accggagcac cacggcgaag gacatcttga aaacgttcac ccacagcatc gcgatccccg
     6541 ggtgcaacaa cgctctgacg gcaggggaat tccttctcct cctgggcccg gaacatgcgg
     6601 ccaccatcgc gggggacggc ttttcgaagg aagatgttaa aaagttcctt tatgaggagg
     6661 cccgcgtccc ggtggaacat ttccccgcgg tgagctggcc gtggatccgt cagatgagac
     6721 ctcaatgggc ggaagtcatc gacaaaaagg ggaagattcc cctcgttgac cgctgggagg
     6781 agatccaggt cgcggtcgcc ggaggagcgg ggaaacattc cgcttttatc cctacctttg
     6841 gagccaccaa accggtaacc aaggctgtcg tccggaaaga cggccgaccg attcgctcca
     6901 tcaaagaatt caaggagaaa aaagggtagg aacaagagga ccttgcatcg taacgaaagg
     6961 aggaataccc atggcagcgt atttggttac ggaaatcgaa gtcacggacc ctgcgggtta
     7021 cgaggaatac cgcaagggag tcggtgccac gctcgccgct tacggggggc gcaacctggt
     7081 tcggcccggc gggaagatag aagtgctgga aggcgattgg gtgcccaagc gtttggtgat
     7141 ccttgaattt ccgagcatcg ccaaactcaa ggcctggtat gagtcgcccg aatatcaacc
     7201 ccttctcaag ataaggcttc ggaccgcgaa gtcgaaaatg gttctgatcg aaggggctta
     7261 gggtccgccc gcaggtgctg gacgggacgc ggcatttgga cccagaaggc ttcacagcct
     7321 agctggaggg actcgaccgg acctgccggg ggcttttctc aagggaaaga acctgagtct
     7381 gctttcagcg accgggggtc atttgcctgg gcagccaaag ggccaaatcg gggaagaaga
     7441 atagaatgcc cacgaatatg atcatcatta tgacataggg agtcgatccc cacgcaatct
     7501 ccttcatcgt gctcccctta gccacccctt ggatgacaaa gaggttgatc cccaccggcg
     7561 gggtgataaa gcccatctcc agccagacga ccataacgat cccgaaccag atgccgtcga
     7621 agcccaaccc ggtgaccagg ggaaagagga agggaagcat gatgatcatc accgagagtt
     7681 cttccagggc ataggcgatc aggatgagca gcagaaagat aagggcaaaa accccccagg
     7741 ggggcaatcc cagatcctga atcagctgga agatggcctt gggggcgccg aggagggcga
     7801 ccacatggga aaagatcgag gcaaaagtga tgatgagcag gcacatggaa gtggggccaa
     7861 tggtgtcgcg gaaggcattc accagggtat tccaactcag ccgtcgatat cccgcggcca
     7921 ggatcatgct ggcaaaggct cccagggccg ccgcttcggt aggggtcacc actccgaaat
     7981 acatggtgcc gagcacgatg aagatgagcc cgaccatggg agcggcttcc gcaagcgaat
     8041 agaaacggtc gcgccaatag actctctccg ttaccttggg agccaattcc gggtgaaggg
     8101 cgcagcggac gatgatgtag ccggcgaaga agatgctcag caggatcccc ggaataatcc
     8161 cggccacata gagctggccg acggaggtct cggtcatgac gccgtagatg atcatgggga
     8221 tggagggagg gatcagaatc cccaaagttc ccccggcagc catcgaaccg tacatcagtt
     8281 ctttctggta accccgtttc tccatttcgg gtatggcaat caacccgatc gtggcggcgg
     8341 tggccacgct agagccgcag accgcaccaa agatggcaca ggcaaaaaca ctagctaccg
     8401 ccagcccccc cggcaggaag gatacccatc gcgtcactgc cgtgtagagg gaagaaccga
     8461 ttcccgagtg aagaaggata gcactcataa agatgaagag cgggagggcg atatagttga
     8521 agttggaggc cgtggaccaa gcgatggagc cgattgcgga catgcccacc acccatcctt
     8581 ttagccagag cagacctaga atccccacca gcccgagaga aagagctacc tccagcccga
     8641 tggataaaac gaaaaagaga aggccgatca ggatcgtgcc gaccagaaaa tcactcaatg
     8701 ggaaatctcc atggaaatgt ctttagcgtc ttcccggccc ttttctacct tccccttcat
     8761 ctcattgacc agttgcaccc ccatgaaaaa aaccatgagg ataaggccca gggggataaa
     8821 aatcatgccc acccacaagg gatagttcat gaccgcggaa acccggcccg tctgcaaggc
     8881 cgagacggtg ctcaccatcg tttcgtagat gacaatgatt aagaaggcga tacccatgaa
     8941 gagggtaatg actctcatcc gcctctgggt cttggatttg aggtggctgg tcacgaggtc
     9001 tatccgaatg tggccgcctc tataaaagac cgggccggcc cccaggaaga tcatcgcgac
     9061 cagcagaaaa gaggtcaagt catctacaaa aagctggggc tcgccgagaa aataacgcat
     9121 taagacatca tagcttacca tcagagtcat agccagaacc gccagcccgg taagcagaat
     9181 gatccactcc gaaaatcgct ccagaacgtt cctcttttta ctgtccatcg gggaaatggg
     9241 ggttccctcc atctttgaat ggccgattac acgctttcgg ggcggtcgtc catccttttc
     9301 ggtgtttccc cctcccccag gggcagggga ggggttgcgg aaagctctgc gggttacgct
     9361 ctcagttgcc aggataaagg ttggacagca gcgttgttcc gcgggggcta gcgtccaagg
     9421 gctttcgcgg cgaggtccag ggcccgttga ccctcggcgc cggtccgctt gagccagtta
     9481 tcccaggcgg gtttgaccat tttgcgggct ttctcgatct ccgccgggga gacatccacg
     9541 atcgtcaatc ccagctccgc gcagcgtttc ttcgccctgg cgtcccaggc tttggcctcc
     9601 tcccattcct tgtcttcgaa gcgggattcc ttgaccgcat ccaggaccac ctgctgcagg
     9661 tcggggggaa gggcattcaa ggccttcttg ttgcagccga gaaattcgat tcccgcgccg
     9721 ctgatgaacc agtagttgat gaacttcgtc acctcgaaga atttcatggg ctcggcattg
     9781 gtggccgagg tgatggctcc gtcggccacg tttctctgca gggccgtata aacctcgcca
     9841 aaaggaatgt tgaccggcgc ccctcccaga gccttggtca gttccgcggt ttcagagccg
     9901 tagactcgaa tcttagctcc cttccagtct tctaccttgg cgaagggttt cttggtactg
//
LOCUS       DASPRR010000603        11004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_149285, whole genome shotgun sequence.
ACCESSION   DASPRR010000603 DASPRR010000000
VERSION     DASPRR010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11004)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149285"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(546..1838)
                     /gene="hydF"
                     /locus_tag="VEL68_22160"
     CDS             complement(546..1838)
                     /gene="hydF"
                     /locus_tag="VEL68_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HXZ38752.1"
                     /translation="MKKTPKSLRLQIGLFGRTNVGKSSFLNLVAGQDVAITSPVAGTT
                     TDVVEKSMELLPLGPVVFLDTAGLDDRSVLAEARLRKTLKIFDRSDIITLVVEPNTWT
                     DYEEKTLEEAKKREIPTILVVNKIDLGISSPEFMGELHSKTSRVLLCSSVDFEHRDPI
                     LNQYKKFLIEVCPDDFLKPPPLVGDLLPEAGLAVLIVPIDLQAPKGRLILPQVQTIRE
                     ALDFDASVMVVKEREYAHSLANLKSHPHLVICDSQVVLKMVADTPEGIACTTFSILFA
                     RNKGDLVELARGAAIIETLKPGDRVLIAEACTHHAIEDDIGRVKIPRWIRQYVGGDLR
                     IDVFSGRDYPDHLQEYKLIIHCGGCMLTRREMLTRIQKARETKVPITNYGLSISFSQG
                     VIRRVLSPFPAALEAFQREMEKMRRTVKSPQSLQSSLS"
     gene            complement(1822..3183)
                     /locus_tag="VEL68_22165"
     CDS             complement(1822..3183)
                     /locus_tag="VEL68_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HXZ38753.1"
                     /translation="MGEKEVPGEAYWGIHTQRALENFPFSGSRVNPSLIRSLATVKKA
                     CGLANLELGYLDERKGKAILAACEEVIEGKLYDQFPVDALQGGAGTSSNMNMNEVVAN
                     RAVELLGGERGDHSMVHPIEDVNLHQSTNDVYPTAVKVSAILLLRELSQTIARVQGSF
                     QRKEKEFARIVKVAKTELQEAVPITLGAEFSAFGEAMARDRWRTFKCEERLRVVNLGG
                     TAVGTGLNAPRSYIFLAVEKLREVTGLGLSRGENLLDATANTDPFVEVSGILKAHASS
                     LGKIANDLRLLHLFGEIELPKLQPGSSIMPGKVNPVVLEAVMQVALRVMANDFLIAEA
                     CSRSTLQINEFLPLLAHSLLESLDLLIRTDEVLARHADGITANETRCRESFDRSPMIV
                     TAFLPHIGYDRASELLQAFRASGRKNVREFLQEKLGMEMVERALSPERLISLGYREDE
                     ENA"
     gene            complement(3346..4728)
                     /locus_tag="VEL68_22170"
     CDS             complement(3346..4728)
                     /locus_tag="VEL68_22170"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HXZ38754.1"
                     /translation="MNPQPFLDLLKEHGQNHILDHYQGLNPVKRQAFVKGLAQLDFSL
                     AFKLYKEFSRQKNSGFLPSQIEPAFIIPIPKTAGEVHQRKEAVKLGESLIRRNQVAVL
                     IVAGGQGTRLGFPGPKGSLPISSIKRKTLFQVFAESIRAISNRYQAALPLLIMTSQEN
                     HEETQAFFEAHGFFSLNRNQVHFFSQGMLPTLTPAGELILKDAENLLANPDGHGGSLK
                     ALYESGLTGWLKGQGVSEIFYCQVDNPLAKIADPAFIGVHRQEKADISTKVVRRRDLE
                     EKVGIYGRVNGKPAIIEYSDFSPDDYRSLDERGNIRYWAGNIAIHMISLSFAERMNRE
                     GFALPYHRAIKEVEGLRPGGRSERMKGLKFETFVFDSIPLAQKSACMEVAREEEFAPV
                     KNQTGADSPDTARQAMTNLFTQWLIEAGAQVSPGARVEISPLFALDKEEVMEKLKGKR
                     VEVREDTYFG"
     gene            complement(4883..5788)
                     /locus_tag="VEL68_22175"
     CDS             complement(4883..5788)
                     /locus_tag="VEL68_22175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase response regulator"
                     /protein_id="HXZ38755.1"
                     /translation="MPGQGKETPTAVLLIEDDPYDAELTMAVLGEIRSFPCMVERAET
                     LSRGLERLSRGGVDLVLLDLGLPDSLGLGTFEMAYMQYPDIPIIVLSGLADEEMAIKT
                     VQQGAQDYLVKGNFDANVLARSIRYAIERQRIKAELKSLSLTDELTGLYNRRGFLTLA
                     RQQIKIAFRLKKRLLLIYSDMDNLKWINDTLGHEEGDRALKEMAEIMSSTFRASDLVA
                     RLGGDEFAVLGLEEGAADFSKIRSRLRQEPRSPGKESARPYRLSFSMGFVQYDPEKPE
                     DIDELLARADKLMYLEKSIKKPKRG"
     gene            6532..8235
                     /locus_tag="VEL68_22180"
     CDS             6532..8235
                     /locus_tag="VEL68_22180"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ38756.1"
                     /translation="MYLEFYGFRENPFNLTPDPRFVFLSKTHREAFAHLLYGIQNRAG
                     FIALTGEVGSGKTTVLRTFLSNLDADHYRTALIFNPLQSPGDLLQSINRELGIPPPTS
                     LGESPLDALNQFLLRENASGRTVVLVVDESQDLEPAALEQVRLVSNLETDRAKLIQIV
                     LAGQPELAQMLRRKEIRQLSQRIIVAYHLQPMDFQDTVHYIMHRLKVAGARGGTFFSK
                     WALKRIYRYSGGLPRLINAACDRALLVGFTLEASKITSRLAAEAIKDLKKETAFYSRK
                     GRMVLIPLFVFLGVVLAAGLYLAGLGSSDPAYYLNRLWGKGGPQGEAHLGAQEEPSRA
                     TAAELASVSDSVATREEFFRAMVSELGSISESESARRSFNTLAGLWNAKPIPETTPWK
                     SSDGMERAALDRNLLLYRFSGNLGALLRIDYPAALELALPGVEGKRFLSLVGMEDEQI
                     KIEPPVGGRSSLAFSEVERYWSGHGFLLWKDSLNLLAKVSSGSRGESVKRFQDLLRET
                     GVYRQPLTGIYGNETLSAVKEFQSSKGIEKDGIVGRQTLMHLYRSLDRFGVPSLSAGR
                     K"
     gene            complement(8408..8632)
                     /locus_tag="VEL68_22185"
     CDS             complement(8408..8632)
                     /locus_tag="VEL68_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38757.1"
                     /translation="MIEGGEVFALSPGSWAAGGTSLLAKEGFSGAGGDILKKGSGSRV
                     AGVTCSLEGDNFADGRDADFSRDPDPTTAR"
     gene            8979..9230
                     /locus_tag="VEL68_22190"
     CDS             8979..9230
                     /locus_tag="VEL68_22190"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HXZ38758.1"
                     /translation="MDRSPRIPQKSAETGVSGPPSSLPQLKISGIMWHDEPSMRRAVI
                     NGTFTNEGSTIEGVEVVEILPTRVRFLYQGRPFEISIFE"
     gene            9604..10074
                     /locus_tag="VEL68_22195"
                     /pseudo
     CDS             9604..10074
                     /locus_tag="VEL68_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(10456..>11004)
                     /locus_tag="VEL68_22200"
     CDS             complement(10456..>11004)
                     /locus_tag="VEL68_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HXZ38759.1"
                     /translation="NGSPRKDGNTSILIKHVFSELERQSIGTELVQLSEKEIRGCIAC
                     YKCIENKDRRCAVKNDAANEYIEKMLGAEGMILGSPVYFNDVTPEMKALIDRTGYVAR
                     ANGRMFKNKVGASVAAVRRSGAVHTLDTINHFFLSGEMIIAGRVIGVGRERGEVEKDE
                     EGMQLAKTLGQRMAWLLKKIHA"
BASE COUNT         2364 a         3131 c         2892 g         2617 t
ORIGIN      
        1 tcgcttccct ttttcaggct tcccgccccg aaggtcccaa gaaggatgaa aaggatcaaa
       61 cagctgtaga agattgattt cccattaccc cgttttccct ttagccagag aaaatcgcgg
      121 agccatttct tccttcctgg attctccgtg atttccctca tctccagagc ctcttgcaaa
      181 agccgcatac tccgtcattc cggcgaaagc cggaatccag aattctttga aaatcctgga
      241 ttccgggtcg cgcttccctt gcccggaatg acaacttctc ctgttttcga gagtttcgca
      301 agaacctctc cattctccgc tttcgtgccc cagaaagggg tctcgcgatg gggaaaaaga
      361 aaaattaaag agccatcctc ccgattatcc tgataaccga tcaaaattac atgagtattt
      421 tagcatatcg taaagatatt tcataatgca agctttatgc cagttaagac gaggctcttt
      481 cgagtcagtt tgtgcccggg gagggtttaa aagtactttt tgcagccatc ggcaattccc
      541 tcctttcaag aaagggagga ctggagagat tggggggact ttaccgttcg gcgcatcttt
      601 tccatctctc gctgaaaggc ttcgagggcc gcggggaatg gagagagaac tcttcggatg
      661 actccctggg agaaggaaat agagagacca taattggtga tcgggacttt tgtttccctg
      721 gccttctgga ttctggtcag catttctctg cgggtcagca tgcaaccgcc gcagtggatg
      781 atcagtttgt attcttgcag atggtccgga taatcccgcc cggaaaacac atcgatccga
      841 aggtccccgc cgacatactg cctgatccat cgaggaattt tgactcgccc gatgtcgtct
      901 tcaatggcgt gatgggtgca ggcctcggca atcagcactc gatccccggg cttcagggtt
      961 tcgatgatcg ccgctccccg ggcgagctcc accaggtctc ccttgtttcg ggcaaagagg
     1021 atggagaaag tggtgcaggc aattccctcc ggcgtgtcgg caaccatttt caaaaccacc
     1081 tgggagtcac aaatgaccag gtggggatgg cttttcagat tggccaggga atgggcgtat
     1141 tctctttctt tgaccaccat aaccgaagca tcaaaatcga gagcttcccg gatggtctgg
     1201 acctggggaa ggatcaaccg ccctttgggg gcctggaggt caatggggac aatcaagacc
     1261 gccagccctg cctcgggaag gaggtctccc accaaaggag gaggtttcag aaaatcatcg
     1321 gggcagacct cgatgaggaa tttcttatac tggttgagga tcgggtcccg gtgttcaaag
     1381 tccacgctgg agcacaagag aacccgggag gttttggagt gcagttcacc catgaattcc
     1441 ggtgaagaaa ttcccaggtc gatcttattg actaccagga tcgtcggtat ctccctcttc
     1501 ttggcctctt cgagagtctt ttcttcatag tcggtccagg tattcggctc gaccaccagc
     1561 gtgatgatat ccgaccggtc gaagattttg agcgtctttc tcaggcgtgc ctccgccagg
     1621 accgaccggt catcgaggcc cgccgtatcc aggaagacga ccgggcccag gggcagaagc
     1681 tccatggatt tttccaccac gtccgtagtg gtcccggcaa cgggcgaggt gatggccaca
     1741 tcctgcccgg ccaccaggtt caggaagctg gactttccca cgttggtccg gccgaagaga
     1801 ccgatctgca accgcaggga tttaggcgtt ttcttcatct tcccgatacc ccagggaaat
     1861 caacctttcg ggggaaagag ctctctccac catttccatc cccagtttct cctggaggaa
     1921 ttcccggacg ttcttccttc ccgaagcccg gaacgcttga aggagttcgc ttgcccggtc
     1981 atacccgata tggggaagga aagcggtcac gatcatcggg ctgcggtcaa agctttcccg
     2041 gcacctggtt tcgtttgccg tgatcccgtc tgcgtgccgg gccaagactt cgtccgtccg
     2101 gatgaggagg tccagggatt cgaggagcga atgggccagg aggggcagaa attcattgat
     2161 ctgcaatgtg ctccgggaac aggcttccgc gatcaggaag tcgttggcca tcaccctcag
     2221 ggccacctgc ataaccgcct ccagcaccac cgggttgacc tttccgggca tgatcgaaga
     2281 cccgggctgc agcttgggaa gctcgatttc gccgaagaga tggagcagcc tcaagtcatt
     2341 ggcgatcttg cccaagctcg aggcatgcgc cttcaggatc ccggaaactt ccacaaaggg
     2401 gtcggtgttt gccgtcgcat ccaggaggtt ttctccccgg gagaggccaa gcccggtgac
     2461 ctcccgcagt ttttcaacgg ctaagaaaat gtagctccgg ggggcgttca agccggttcc
     2521 cacggccgtg cctcccaggt tgacgacccg gagcctttcc tcgcatttga acgtcctcca
     2581 tcggtcccgg gccatcgcct cgccgaaggc ggaaaactct gcgcccaagg tgatgggcac
     2641 cgcttcctgg agttcggtct tggctacctt tacaatcctg gcaaactcct tttccttcct
     2701 ctgaaacgaa ccctgaaccc gggcgatggt ctggctcagt tcccggagga gaaggatggc
     2761 cgataccttt acggctgtgg ggtagacatc gttggtggac tggtgcagat tcacatcctc
     2821 gatggggtgg accatcgagt ggtcacccct ctctcctccc agcaattcta cagcccggtt
     2881 ggccaccact tcattcatat tcatgttgct ggaagtcccc gctccgcctt ggagagcgtc
     2941 aacgggaaat tgatcataga gctttccttc gatcacctct tcacaggccg caaggatggc
     3001 cttccctttc ctttcgtcca aataccccag ttccagattg gccaagccgc aggctttctt
     3061 taccgtggcc agagacctta tcagcgaagg atttacccgc gatccgctga agggaaagtt
     3121 ttccaaggcc cgctgggtat ggatgcccca gtaggcctcc cccggaacct ccttttctcc
     3181 caagaaatcc ttttccctgc ggtatttcaa atcgatgatt gtcccttgtg cgttgccggt
     3241 tactggttgc tcattgctcg atgctggttg cggggcgctt cgtccttctt gggagactcg
     3301 gggtcacccg attgggctcc agtctccatg ccttccgtca aatccttacc cgaaatatgt
     3361 gtcctcccgg acttctaccc tcttcccttt cagtttttcc atcacctctt ccttgtccaa
     3421 ggcaaaaagg gggctgatct ccactcgggc tccgggggaa acctgggctc ctgcctcgat
     3481 cagccattgg gtgaaaaggt tcgtcatggc ctgccgggca gtgtctggag aatcagcgcc
     3541 ggtttggttc tttaccggtg caaattcctc ttcccgggca acctccatgc aggcgctttt
     3601 ttgcgccagg ggaatggagt cgaagacaaa ggtttcaaat ttcaggccct tcatcctttc
     3661 gctcctgccc cccggtcgca ggccttccac ttccttgatc gcccggtgat aaggaagggc
     3721 aaacccctcc cggttcatcc gttccgcgaa ggagagggag atcatatgaa tggcgatatt
     3781 ccccgcccaa tagcgaatgt ttcctctctc atcgagagac cggtaatcat ccggagaaaa
     3841 gtcactgtat tcaatgatgg ccggtttgcc gttcaccctt ccatagattc ccaccttctc
     3901 ttccaggtcc cgacgtcgga caaccttggt ggaaatatcg gctttttcct gccgatgaac
     3961 tccgataaaa gccggatcgg cgatcttcgc caggggattg tccacttggc aataaaatat
     4021 ttcggagacc ccctgccctt tcaaccatcc ggttagcccg gattcgtaga gggctttcag
     4081 agaccccccg tggccgtccg gattggccaa gagattctcc gcatctttga gaatgagttc
     4141 tccagcaggg gtcaaggtcg ggagcatccc ctggctgaaa aaatggacct ggttcctgtt
     4201 caggctgaaa aagccgtggg cttcgaagaa ggcttgggtt tcttcgtggt tctcctgact
     4261 ggtcatgatc aggagcggaa gagccgcttg gtatcggttc gaaatagccc ggatcgattc
     4321 ggcgaagacc tgaaacaagg ttttcctctt tatagaggaa ataggaagac tccccttggg
     4381 acccggaaat cccagtcgag tcccctgccc tccggccaca atcagaacgg caacctgatt
     4441 cctgcggatc aaggattcgc caagcttcac agcctctttc cgttgatgaa cctctcccgc
     4501 agtcttgggg atggggatga tgaaagccgg ttcaatctgg gagggaagaa aacccgagtt
     4561 cttttgccgg gaaaactcct tgtagagctt gaaggccaag gagaaatcaa gctgggccaa
     4621 acccttgaca aaagcttgcc gcttcactgg gtttaacccc tgatagtggt ccaggatatg
     4681 attttgacca tgctctttca gcaggtccag gaagggttgg gggttcattt ttcttccttg
     4741 gatgggtctt tatctttccg agctaattat aaaattgaaa tgatttgggg tcgagaaaaa
     4801 agtgaaaggg gctgcaattc agactgtaag aaaggaacgg agggggagga tcagagaccg
     4861 gagatttctc gagcagcggc ttctatcctc tcttaggctt cttaatgctt ttctccaagt
     4921 acataagctt gtccgcgcgg gccaggagtt catcgatgtc ttccggcttt tcaggatcat
     4981 actggacaaa gcccatgctg aaggataggc gatagggcct tgccgattct tttccggggg
     5041 agcggggttc ttgtcgaagg cgcgacctga ttttagaaaa atcggccgct ccctcttcca
     5101 gccccaggac ggcgaactcg tctcccccca agcgagccac caggtcggaa gcccgaaacg
     5161 tgctgctcat gatttccgcc atttccttca aagcccgatc cccttcttcg tggcccaggg
     5221 tgtcgttgat ccacttcagg ttatccatgt ccgaatagat gagcaagagc ctcttcttaa
     5281 gccgaaaggc gatttttatc tgttgccgag ccaaggttag gaatccgcga cggttgtaca
     5341 gcccggtgag ctcgtcggtg agagacaggg acttgagttc ggccttgatc ctctggcgtt
     5401 cgatggcgta ccggatggag cgggccagga cgttggcatc gaaatttccc ttcaccagat
     5461 aatcctgtgc cccttgctgc acggttttga tagccatctc ctcatccgcc aggccgctca
     5521 ggacgatgat cgggatatcg ggatactgca tgtaggccat ttcgaaggtg ccaagtccca
     5581 gggaatctgg aagccccagg tccagcaata ccagatcgac tcccccgcgg gagaggcgct
     5641 ccagccccct ggaaagcgtt tccgctcgct caaccatgca tgggaaactt ctgatctccc
     5701 ccagaaccgc catcgtgagt tcggcgtcat atgggtcatc ctcgatcaaa agaacggccg
     5761 tgggggtctc ttttccttgt ccgggcatag aacgacctcc tctcgaacaa gacacagact
     5821 acatgattac tataaattcc tggagttttc aaccggccct ggttcttgcc cacccccagt
     5881 ttttcgtgct tgttggggtt cagggtggag gaatttggat tatcagactc ggcgggtgct
     5941 tatccttcca tgagactcct ccccatgctc cgcagggaga ggcctgagag gagatccctc
     6001 caagcagaaa agagagccaa agcatgtttc cctctcaccc cccccaggac aggcccgatc
     6061 caatgaaaga accaccgcct caggatgatg atccaaattt ctccgccctg aaccccatgc
     6121 gatcctcccc tcctttccca aggtcgtatt ttcgaaggac tctttttcga gaactggggg
     6181 tgggcaaggg ccctggcttg tccctaatcc gcccatggat gctcggggat atgtcatttt
     6241 tttgtccatt tcttttccgg aaaagaggca gagccgggtt ccgctcctct tggcaggtat
     6301 tcttttctta ttgccggtgg gtttgataaa tggttatatc gaaaggaaca taaaccctgc
     6361 aattctttgc ccatttaacc gattaagcat tttcccagaa aatttggcat gaaatctgct
     6421 attataggac taagccgagc gtgggggttt atttccctat tcggatatta tagttttctc
     6481 cttttttctc tccttgcctg gagacgggca gggattgagg ggtgtcttct gatgtacctc
     6541 gaattttacg gctttcggga aaaccctttt aatttgactc ccgatcctcg ttttgtcttt
     6601 ctgagcaaga ctcaccggga agcgtttgcc caccttctgt acggcatcca aaatcgcgcg
     6661 ggattcatcg cccttacggg agaggttggg tcggggaaaa ccacggtttt gcgcactttc
     6721 ctgagtaatc ttgacgcgga tcattaccgc acggcattga tcttcaatcc ccttcaatct
     6781 cccggtgatc tgcttcaaag cataaatcgg gaacttggga tcccgccccc cacttccctg
     6841 ggggagagcc ccctggacgc cctgaaccag tttcttctcc gagagaatgc ctcgggccgc
     6901 accgttgtcc tggtcgtcga cgagtcgcaa gacctcgaac cggcggcgct cgagcaggtc
     6961 cgcttggtct ccaacctgga gacagaccgg gcaaagctca tccaaatcgt ccttgccggg
     7021 cagccggaac tcgcgcagat gcttaggcgg aaagaaatac gccagctcag ccagcgcatc
     7081 atcgtggctt accacctgca gcctatggat ttccaggata ccgtccatta catcatgcac
     7141 cggctcaagg tggccggagc aaggggtggg actttctttt caaaatgggc tctgaagcgg
     7201 atttatcggt attccggggg acttccaagg ctcatcaatg cagcctgcga ccgggctctt
     7261 ttggtcggat tcaccctgga agcatccaag atcacctccc gccttgccgc cgaggcgatc
     7321 aaagatttaa aaaaagaaac agccttctac tctcggaaag gacgcatggt tctgattccc
     7381 ctctttgtat ttctgggggt cgtcctggcc gcgggattgt atcttgccgg tcttggctcc
     7441 tctgatccgg cctattactt aaaccgcctt tggggaaagg gggggccgca gggagaagcg
     7501 catcttggtg cccaggaaga accttcccgt gccacagcgg ctgaactcgc cagcgtttcc
     7561 gattccgtgg ccacccggga agaattcttc cgtgccatgg tgtctgaact cggcagcatt
     7621 tcagaatcgg aaagtgcccg taggtctttt aatacgctgg ccggcctctg gaatgcgaag
     7681 cccatcccgg aaacgactcc ctggaaatcg tccgatggaa tggaacgggc cgccctggat
     7741 cggaatctcc ttttgtatcg gttttccggg aacttgggcg ccctgctgcg catcgattat
     7801 ccggcggccc ttgaactggc cctccccggt gtggagggga aacgcttcct ttcgctggta
     7861 ggcatggagg atgaacaaat caagatcgag ccgcccgtag gagggagaag ttccctggct
     7921 ttcagtgaag tggagaggta ctggtccggg cacggatttc tcctctggaa agactccctt
     7981 aacctcctgg ccaaggtgtc ctcggggtca aggggggagt ccgtcaaacg gtttcaggac
     8041 ctcctccgag aaacaggggt gtatcggcaa cccttgacgg gtatctacgg taacgaaacc
     8101 ctttcggcag tgaaagagtt ccagtcctcc aagggaattg aaaaggacgg tatcgtaggt
     8161 cgtcaaaccc tgatgcacct gtatcgttcc ctcgaccgct tcggagttcc aagtctcagt
     8221 gcaggaagaa aatgagcctg atcctcgacg ccctgagaaa acttgaccgt caaaaatcct
     8281 taaaccggaa gggaacggtt gaccttgccg cttccatcct tagcccggat gcttcgcctc
     8341 cccgaaaaaa atggaggttc tatttagttg ctgtctctct caccgccgtg gtcacagcgt
     8401 cgatcaccta cctggcggtt gtggggtccg ggtctcggga gaaatcggca tctcttccat
     8461 ccgcaaaatt atccccttcc agcgagcagg taacccctgc tacccgtgag cccgagccct
     8521 ttttcaaaat atcgccgccc gccccggaga agccttcttt ggccagcagg gacgttccac
     8581 ccgctgccca ggagcccggc gaaagagcga aaacctcccc tccttcaatc acggctcccc
     8641 gggcatctca tccgcaatcc gaaccggtct cgtcggcagc cgcacccccg caaaaagtac
     8701 tcacgggtga ttccaccata tcccgagggt ccagtcagcg cgttgcgatt tctccttctg
     8761 gatccgggag cccggcagag tcatctctcc ccgcctctga agtccctatt aaaataaatc
     8821 ctccccctgc tcctgactct ctccctcgcg aaacggtgcg tgaggttcaa agcgggaaga
     8881 ccgaggagac accaaaaata gagacccctc ccggaactat agcttctccc cctgccgcgg
     8941 gtgaaaaaat atccgaccag aaggcggttc cggaaaaggt ggaccgatcc cccagaattc
     9001 cccaaaaatc agctgaaacc ggggtcagtg gacccccttc ctccctgccg caattgaaaa
     9061 tttccgggat tatgtggcac gacgagcctt ccatgcgccg cgctgtgatc aacggcactt
     9121 tcaccaacga gggttcgacc atcgaaggcg tggaggttgt cgagatcctt cctacacggg
     9181 ttcgcttcct gtatcaaggc cgccctttcg aaatttccat ttttgagtaa gaatcgaccg
     9241 ccctgccggg tgaccaatcc tcatcgatgg aatcaagctc cctttcaaca taaaagagcc
     9301 tttcgctcct tttctcatca tcgggacaac agggaattca aattcgttcc cttgtttgcc
     9361 ccgggaaaac cttgttccta tccccatcca gtccccttcc cccgggaaag ctggggttgg
     9421 gatgcccaat ccacccccta cctggggaat tgcattcaag aattccctcc ttggtttttg
     9481 ccgttgtttt tggaaacgat tgagggtagt aataatggct gaagatatcg gtcgaaatag
     9541 gatttaagaa aaggggcaga aaagatgaaa cgggtggcaa tcaaagggtt gctgcttatc
     9601 ctcatgtcgg ggatggggtt cctatgggcg gcaggcccgg gattcgccca gtccagggtt
     9661 ccggacgtcc attatgagcc cactcctcag ctcattgtcg aggatttgct caagatggcg
     9721 gaggtgaacc ggaatgatat catctacgac ctgggctgcg gggatggccg attcgtgatc
     9781 accgcggcga aaaaatacgg cgcccgaggg gtggggatcg acatcgatcc ggaacgaatc
     9841 aaggaaagca accagaacgc ccgggtcgcg ggggtcatgg ataaggtgaa atttatagaa
     9901 gcggacctct tcgaaaccga tattcgggag gccaccgttg tcgcccttta cctgcttcca
     9961 gagatgaata tgcagcttcg gcccaaactc ttgaaagacc tgaaaccggg ctcgcgagtt
    10021 gtgtcccatg aattcgatat gtatgattgg ccgccggata aaacaggcaa gctgggccgc
    10081 aacaagtact atttgtgggt agtgccgacg gacgtggccg gcaagtggcg ctggactttc
    10141 ccctcagcca agggggaaga ccaatttctg ctggccctca cccaaaagtt ccaggatatt
    10201 gacggcaaga tcatcactcc cattcaaacc gtgtccccct tggaggccta tttgatggga
    10261 gaccaaatcg gctttctcct gcgatacagg ccttcgggga agacggtgga gatgcgcttc
    10321 cggggccagg taattggaaa caccattatc ggcactgtgg aagtcaatag cggacctttc
    10381 gccggcaaac ataaatggac cgccaggcgc taatccctga gtctctcaaa aaatgaaacg
    10441 atgcgaaatc catccttacg catgtatttt ttttagcagc caggccatcc tttgcccgag
    10501 ggttttcgca agctgcatgc cctcttcgtc cttttctacc tctcccctct cccggcccac
    10561 gccgatcacc cgcccggcga tgatcatctc tccgctgagg aagaaatggt ttatcgtgtc
    10621 cagagtgtga accgcccctg accggcgcac ggcagccact gaggctccga ccttgttctt
    10681 aaacatgcgg ccattggccc tggccacata cccggtgcga tcaatgaggg ccttcatttc
    10741 cggggtgaca tcattaaaat agacgggaga acccaggatc atcccctcgg ccccgagcat
    10801 tttctcaata tactcgttgg ccgcgtcatt cttgaccgca cagcgccggt ctttattttc
    10861 aatgcactta taacaggcaa tgcagccccg gatttctttc tccgaaagct gaaccagttc
    10921 ggtcccgatg ctttgtcttt caagttcgct aaaaacatgt ttgatgagaa tggatgtgtt
    10981 cccatccttc ctggggctgc cgtt
//
LOCUS       DASPRR010000604         5527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1764207, whole genome shotgun sequence.
ACCESSION   DASPRR010000604 DASPRR010000000
VERSION     DASPRR010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764207"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..747)
                     /locus_tag="VEL68_22205"
     CDS             complement(169..747)
                     /locus_tag="VEL68_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HXZ38760.1"
                     /translation="MKEEIAQIFRESADLKLRFVRQNAELLIQVVRMIVEAFKGGKKV
                     LLFGNGGSAADAQHIAAEFVNRFLIERPPLPALALSTDTSVLTSISNDYGYVDSFAKQ
                     IKALGKEGDVAIGISTSGSAANVIKAVKVAKEMGLKTVGLTGNDGGELAKGVDIALIV
                     ASPVTPRIQEVHITVGHVLCEMVDRMLFQHPT"
     gene            complement(791..1600)
                     /locus_tag="VEL68_22210"
     CDS             complement(791..1600)
                     /locus_tag="VEL68_22210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HXZ38761.1"
                     /translation="MNYPVKGFLETSFSDWSGKVVSVLFLPSCNLCCPFCHNHELVRR
                     PEAYDDFPWETILESLRKRKGWIDGVCLTGGEPTLHPWLLPLIQELKSNRNLTSSGEP
                     LAIKLDTNGTHPEILESLLRQGLLDYVAMDLKAPLEADRYSALTGVSFSDERMERVRA
                     SVRLLLRGNVDYEFRTTVVPCLLEEEEIYAIARRIRGAARYTLQNFNPRETLDPRLRE
                     AAPLEPSVLQRMQDRAREIIRNEIPSFEFRVENSRVPVAGSGLGQLDGVAF"
     gene            complement(1661..1906)
                     /locus_tag="VEL68_22215"
     CDS             complement(1661..1906)
                     /locus_tag="VEL68_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38762.1"
                     /translation="MTLFTKEGCQKCEFVKKSVDLKKLGITEEVLGPHNPDSLAHLAW
                     HELVSVAETQLPILVLDDSSSITGAIRIKNYLLAVSV"
     gene            complement(2037..4328)
                     /gene="nrdD"
                     /locus_tag="VEL68_22220"
     CDS             complement(2037..4328)
                     /gene="nrdD"
                     /locus_tag="VEL68_22220"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HXZ38763.1"
                     /translation="MGLGNSQVHQSGTQSAESPEEAMGEPLKSHPPGDGGMGKQGVPS
                     TSSLEPANFRNETTDMALFVRTSAETLSDWNRQRIVEALLRETTIDGDTAERISREVE
                     EMILASKISLVTAPLIRELVDAKLTEYGLEEARKMHTRLGVPLYDVDQLILHPNRENA
                     NVPHGPEATNLTLAERIKKEYALLNVFSQEIGDAHMRGDLHLHDLGFIDRPYCSGQSL
                     EYIKKFGLSLPNSLSMAKPAKHPEVLLAHMVKFAAALQSNFAGAIGWDAVNLFFAPYL
                     DTLSDREVKQLAQMLIYEFSQQAVARGGQAIFTDINLYWEVPKHFEKVMAIGPNGEFT
                     GKTYGQYLDQSQRFVWALFDVFREGDGVGRPFFFPKPLVHITEKFFQTSRHMDFLHHI
                     CDVASERGNTYFVFDRGNTAKVSECCRLSFKLEKSDLEDANYPWRMRYCALQNVTLNL
                     PRIAYQCGGDDTKLFAKINESLHLAVRAHLEKKAFLERLLALGDSGPLALLTMKKDGQ
                     PYLRMHRATFLIGMVGLNEMIQVHRKEQLHESDDTLKFGLKVIAYMKLLADRMSQKYN
                     MRFVLEQTPAESTAYRFAKLDLQAFSPLSGSVVKGDLSRGEVYYTNSTYLNVGTVLNP
                     IDRVRKEGLFHPLIEAGSLTHIWLGESRPSSESLANFVIKVFRHSQNDQIAFSPEFTT
                     CTTCGRTARGLSSQCAYCGSAEVEGITRITGYFTKVSGWNKGKLGELRDRYRSGEYFT
                     VPVSNQGPASIGRGQAGAAGLAA"
     gene            complement(4741..>5527)
                     /locus_tag="VEL68_22225"
     CDS             complement(4741..>5527)
                     /locus_tag="VEL68_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXZ38764.1"
                     /translation="KGVKMPADAVTVVMGINDEHFDPKQHRMISGASCTTTCLAFMVK
                     PLLDYFGADKILTASMVTVHAATGSQEVLDRLPDAKSKDLRKNRSIMNNIILTSTGAA
                     KALALVIPEMKRIGFIAESVRIPTSTGSLIVLVVTLEDESIEHPIKRELINQVYQKAS
                     EGYMKEYLLYSERQNVSSDIIGNPRAAAIIEGSENHTRTAQICIDLAKVPQLKSVDLK
                     TEEGACIAEIPVTQAVIYGWYDNELGSYTNLLGDLTFKIAHSL"
BASE COUNT         1187 a         1446 c         1621 g         1273 t
ORIGIN      
        1 ctgactcagg tggaaccaaa cggattaaag gcatcaaaaa atctgaatat cgaaatccga
       61 aacaaattca aaaagttcaa aaacagcaaa ccaagaaaaa acatttgaat ttggaacatt
      121 cggatttgtt tcggaattcg gatttcgtgc ttcgaatttg ggattttctt atgtgggatg
      181 ctggaacagc atccggtcca ccatttcgca gaggacatgg ccgacggtga tatgcacctc
      241 ctgaatccgg ggggtgaccg gggaggccac aatcagggcg atatctaccc ccttggccaa
      301 ttcccccccg tcgttgccgg tgaggccgac ggttttcagg cccatttctt tggccacttt
      361 aacggctttg atgacattgg ccgcggaacc gctggtggag atcccgatgg ccacgtctcc
      421 ctctttcccc agggctttga tctgcttggc aaaggagtcc acgtacccgt agtcgttgct
      481 gatgctggtc aggactgagg tgtcggtgga caaggccaag gcgggcagcg ggggtcgttc
      541 gatgaggaag cggttgacga attccgcggc gatgtgctgg gcatcggcgg cgctcccccc
      601 attgccgaag aggaggacct ttttgccgcc tttgaaggct tctacgatca tccggaccac
      661 ctggatcaga agctcggcgt tctgccggac aaaacgcagc ttgaggtcgg cgctttcccg
      721 gaagatctgg gcgatttcct ctttcattcc caaacccctt gcttcgagtt ccgggtttcg
      781 ggtttcaggt tcaaaatgcg accccgtcta actgccctaa cccggagcct gcaaccggaa
      841 cccgggaatt ttcaacccgg aactcgaaac tgggaatttc atttcttatg atctctcggg
      901 ctcgatcctg catccgctgg agaacggacg gttccagggg cgccgcttcc ctcagccgag
      961 ggtcgagggt ctcccggggg ttgaagttct gcagggtata ccgggccgcg ccccgaattc
     1021 ggcgggcgat ggcgtaaatc tcttcttcct ccagtaagca tggaaccacg gtggtgcgaa
     1081 attcataatc cacattcccg cggagtaaga gccggacact ggcccggacc ctctccatgc
     1141 gctcatcact gaatgaaacg ccggtcaggg cggaataccg gtccgcttcc aggggggctt
     1201 tgaggtccat ggccacataa tccaaaagcc cctggcgcag gaggctctcc agaatttccg
     1261 gatgggtccc gttggtgtcc agcttgatcg ctaagggttc tccagaagaa gtcaaattcc
     1321 gattcgactt cagctcttgg atcaacggca agagccaggg atggagggtg ggctcccctc
     1381 cggtcagaca gaccccatcg atccacccct ttcgtttccg caggctctcc aggatggttt
     1441 cccagggaaa atcatcatag gcctcgggcc ggcggaccag ttcgtggtta tggcaaaagg
     1501 ggcagcagag attgcaggag gggaggaaga ggactgagac gactttcccg gaccaatcgg
     1561 aaaaagaagt ctcaaggaac cccttgactg gatagttcat gataacttac ggctttcagc
     1621 gatcagcttg caggggcggt tcgcgaaccg cccctacaga ctaaacgctg accgctagaa
     1681 ggtagttctt gattcggatg gccccggtaa tggaagagga atcatccaaa accaggatcg
     1741 ggagctgagt ttcggccacc gagaccagtt catgccaggc caggtgggcc aaggaatcag
     1801 ggttgtgcgg acccaggact tcttcggtaa tccctagctt tttcaagtcc acgctctttt
     1861 ttacaaactc gcatttctgg cagccttcct tggtaaaaag ggtcaattcc tccacctcca
     1921 ttcaaaattt atcgggatgc agatatacgc aggtgaaggc agagaaataa ataaacttaa
     1981 gcccttttct taagaccctg aaaatctgcg ttcatctgcg tcccatctta ttgaggttaa
     2041 gctgccaggc ccgccgcgcc ggcctggccc cgcccaatcg aggccggtcc ctggttcgaa
     2101 acggggacgg tgaagtattc tccgctccgg tagcggtccc gcagctcacc cagtttccct
     2161 ttgttccagc ccgagacttt ggtgaaatac cccgtgatcc gggtgattcc ttccacttcg
     2221 gcggaaccgc aataggcaca ctgactgctc agaccgcggg ccgttcgccc gcaggtggtg
     2281 cacgtggtga actcgggaga gaaggcgatc tggtcgttct gcgaatgtcg gaagactttg
     2341 atcacaaagt tggccagaga ttcactggaa ggccggctct ctcccagcca gatgtgggtc
     2401 agggaaccgg cctcgatcaa ggggtggaag agaccttcct tgcggacccg gtcgatggga
     2461 ttcaaaaccg tccccacgtt gagataggtg gagttggtgt aataaacctc tccccgggag
     2521 aggtctccct taaccaccga gcccgagagg ggagagaaag cctgcaggtc caacttggca
     2581 aaacggtagg ccgtgctctc ggccggggtc tgttccagga cgaagcgcat attgtacttc
     2641 tggctcatgc ggtcggccag gagtttcata taggcgatca ccttcaggcc gaatttcagg
     2701 gtgtcatctg actcatggag ctgttccttc cggtgcacct ggatcatttc gttcaggccc
     2761 accatgccga tcaggaaggt agcccggtgc atgcgcaggt agggctgccc gtctttcttc
     2821 atggtgagaa gggccaaagg cccgctgtcc cccaaggcca gaaggcgttc caggaaggcc
     2881 ttcttttcca gatgggcccg gaccgccaga tggagggact cgttgatctt ggcgaagagt
     2941 ttggtgtcgt cccctccgca ctggtaagcg atgcggggaa ggttcagggt gacgttctgc
     3001 agggcgcagt accgcattcg ccaggggtag ttggcatctt ccaggtccga tttttccagc
     3061 ttgaagctca gccggcagca ctcggagacc ttggccgtgt tccctcggtc gaagacgaaa
     3121 taggtgtttc ccctttcgga ggccacgtca cagatgtgat ggaggaagtc catgtgtctc
     3181 gaggtctgga agaatttttc ggtgatgtgc accaggggtt tggggaagaa gaaaggccgg
     3241 cccacgccgt ccccttcccg gaagacatcg aagagagccc agacgaagcg ctgggattga
     3301 tccaggtatt gcccgtaggt cttgccagtg aactctccgt tggggccgat ggccatgacc
     3361 ttctcgaagt gcttggggac ttcccagtag aggttgatgt ccgtgaaaat ggcctgtcct
     3421 ccgcgggcca cggcctgctg cgagaattcg tagatgagca tctgggccag ctgctttacc
     3481 tcccggtcgc ttagggtgtc caggtagggg gcgaagaaaa ggttcactgc atcccatccg
     3541 atagccccgg caaagttgct ctggagggcg gcggcgaatt tcaccatgtg ggccagaaga
     3601 acttcgggat gtttggccgg cttggccatg gacagggaat tgggcaggct gaggccgaac
     3661 ttcttgatgt actcgaggga ctggcccgag cagtacggac ggtcgataaa gcccaggtca
     3721 tgaagatgca ggtccccccg catgtgggcg tcgccgatct cctgggagaa aacattgagc
     3781 agggcgtatt ccttcttgat ccgctcggcc agagtcagat tggtggcctc cggcccgtgg
     3841 gggacgttgg cattttcccg gttggggtgg agaatgagct gatccacgtc gtagaggggg
     3901 acccccaggc gggtgtgcat cttgcgggct tcctccagcc cgtactcggt gagcttggca
     3961 tccaccaatt cccgaatcaa cggagctgtg accagggaaa tcttggaggc caagatcatt
     4021 tcctccactt ctcggcttat gcgctcggcc gtatccccgt cgatggtcgt ttcccggagg
     4081 agtgcttcca cgatcctctg ccgattccag tccgagaggg tttccgcaga agtgcggaca
     4141 aagagggcca tgtcagtggt ttcatttctg aaatttgcag gttcaagaga gctggtggaa
     4201 gggacaccct gtttacccat cccgccatcg ccgggaggat ggctttttag gggttcaccc
     4261 atggcttcct ctggggattc ggccgattga gttccgctct gatgaacctg agagtttcct
     4321 aaccccattc aacccctcct tgttcacagc ttatttttta aaaaaattat ggaactgaga
     4381 acctttcctt aataagaagc tttaaaaaat agaattaccg gccaggagaa aagcgaggtc
     4441 ccatctttgt ggggaaatat attgtgcttt aagaattatt aaccactata tatagtggtt
     4501 gtcaagggaa atttatgctt ccgcggaaaa aatttaaaac taattaaaat tgttcaataa
     4561 ttcgatgcat ggggagatta attggagatt aatctccgaa tatgttgggg ctaagaatgg
     4621 tcacgccact agatattgtg ccggttataa aaggacccag agatggggcg gggaaaacag
     4681 tcccctcagg ttcaattgga ttttcgagcc tgaggggaag aggaaggttg atgatagggc
     4741 ttacagagag tgggcgattt taaaagtaag gtctcccaga agattggtgt agctccccag
     4801 ttcgttatcg taccacccgt agatgaccgc ctgggtgact ggaatttcgg ctatgcaggc
     4861 tccctcctcg gttttcaaat caacgctttt gagctggggg accttggcca agtcgatgca
     4921 gatttgggcg gtgcgggtgt gattttccga tccttcgatg atcgccgccg cgcggggatt
     4981 gccaatgatg tcggaggaga cattctgcct ttcactgtac aggagatatt ccttcatgta
     5041 cccctccgag gccttctggt agacctgatt gatcagctcc cgcttgatgg ggtgttctat
     5101 gctttcatcc tccagggtca ccaccaggac gatcagggac ccggtgctgg tgggaatccg
     5161 caccgattcg gcgataaacc cgatgcgttt catctccgga ataaccaggg ccagggcctt
     5221 ggccgctcca gtggaggtga gaatgatgtt gttcatgata gaccggtttt tgcggaggtc
     5281 cttggacttg gcgtcgggca gcctatccag gacctcttgg gaccctgtgg ccgcatggac
     5341 cgtgaccatg gaggcggtga ggatcttgtc cgcaccgaaa taatccagga ggggcttgac
     5401 catgaaggcc aggcaggtgg tggtacagga ggcgccggag atcatccggt gctgtttagg
     5461 gtcgaagtgt tcgtcgttga tgcccatgac cacggttact gcatcagcgg gcattttgac
     5521 ccccttt
//
LOCUS       DASPRR010000605        15045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2244673, whole genome shotgun sequence.
ACCESSION   DASPRR010000605 DASPRR010000000
VERSION     DASPRR010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15045
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244673"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VEL68_22230"
     CDS             <1..497
                     /locus_tag="VEL68_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38765.1"
                     /translation="VSTRYLSDGAVEVTVEIRMASQTAPTDSLAATVLPPQAMGTQSQ
                     NIPKAGNPVYTGVIFDARGLSLRPAMSPKVLDEDGREVYGSAFVSQEWATKFGILGYV
                     KDLEGAKKSDRVAANPLVVKASKVSGTAGCDLVISNADAQGMRDMSKNLSFLEQCRVL
                     IVVD"
     gene            604..1782
                     /locus_tag="VEL68_22235"
     CDS             604..1782
                     /locus_tag="VEL68_22235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38766.1"
                     /translation="MKRRRLLYWLAFLTMVAMLAMLISSCAKPTRKPEAALDTPEHHV
                     FTGYKLYDKGDYEGARREFVLAMQLDKNYSPAYVGLGLCFGSQNDFKAAYENMKKAKS
                     LAKKKEDRVDAHVGMIRLYSMERKDKWLKDAEDEFEDAVKLDPKSSAAYFYMGKAYKF
                     GYEFDKAGTQFKKVLDLNDKYLVEADNEWKVVQKIQRAAPGSMIGKKIALIEEITRAD
                     LAALFIQELLLEKLYQKRTPAQFDTSFQAPTDTRKFEADKIVKAEPATDIQAHVLRAD
                     IEAVLRLGVRGLEPTPDHKFLPYEKVNRAAYAMMIEDILIKVTGNQKLATEYIGSKSP
                     FPDLRNDLPYFNAVMVTTSRGIMEAKDMSTGEFGPRGTVSGADALLIIRKLKDELRFF
                     "
     gene            1799..2986
                     /locus_tag="VEL68_22240"
     CDS             1799..2986
                     /locus_tag="VEL68_22240"
                     /inference="COORDINATES: protein motif:HMM:NF027862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HXZ38767.1"
                     /translation="MGKHVWIGGWLILFLLCGSLPAFPQQAQVVTAEGVGAVIGGDRA
                     IARDQAIQDALRKAVEQAVGTLVSSETMVQNFQTLSDKIYIQSQGYIQNYQIVSETPG
                     PNVHQVTIQATVAMGNLEQDLQALGFLLGQVGKPRIMILVAEQQIGQQYYNYWWGTHR
                     GTQTDLNVAENTIMDRFREKGFDIVDPQAQAGSIKIPPAFQVVELSNQNAITIGKQVD
                     AEIVIVGKALARAAGSIAGTAMKSLQANISMRAIQVDNARVLSSGTENAAAVHIDEVT
                     GGSEAIKKASIKMSDKMMDDILKNFQKRVGATTLVQLMVFGLSGADDVRKFKTSVLGQ
                     VRGVEGIHERSFAENTLKVDVDIRGNAQSLSQEIGRKTFPDFNVKVIRSTWNTLEVQV
                     SPK"
     gene            3078..3722
                     /locus_tag="VEL68_22245"
     CDS             3078..3722
                     /locus_tag="VEL68_22245"
                     /inference="COORDINATES: protein motif:HMM:NF017459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNA1162 family protein"
                     /protein_id="HXZ38768.1"
                     /translation="MKGIERFRWPFLVSVTIFSLACAGGAPHSVVPDYKDRMPRSVAV
                     LPVLNETVSLKAPEVFRPILLNKLSMKGYETPALGFIDGRLREKGVREAGQVNSLTPQ
                     EIGKLLGVDALLYASVTEFSTTYLLAYASMTVGARFELKDTKTGEKLWDSDHQVKESK
                     LGLDQKSMGDTMQFAAGQSYVPYAQKVIDESFKTLPNGPRASAPAQTGCLMPGL"
     gene            3759..4724
                     /locus_tag="VEL68_22250"
     CDS             3759..4724
                     /locus_tag="VEL68_22250"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HXZ38769.1"
                     /translation="MFFKVFQVWSLVLLLLASWGCASMPPGGGGQAQQPQMAQAQPAA
                     QPTVPARQVTHKGPKLRVGIVDFVNKSSYGAGRLGTSASDILTTELFKTGAFIMIERD
                     RLKQVLGEQALGQTGAVSAETAAQAGKVLGLNALVTGSISQFGVSTGGADYGVYKQKV
                     QTAKCTVDVRVVDASTGQLLFADSGKGEFERKAQEVLGLGTRAGYDETLGQEALRSAI
                     TKFMDNLVQKLQSVEWSGAVAMVSGTDVYINAGRDVGLNPGDILMVQTLGQEIVDPQT
                     KVVLGRTRGSVKGELLVTEIDEKFTISKVRSGVGFQVGDLVRIKK"
     gene            4745..5059
                     /locus_tag="VEL68_22255"
     CDS             4745..5059
                     /locus_tag="VEL68_22255"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXZ38770.1"
                     /translation="MRKFSFLLLLGLAVFWGCGMPQETRRGVGQEGFLLIQAVPDDAE
                     VYVDGQLMGKAGKYEKDPLELSGGTHKIEFRKAGFLPEVREVYVGNQSRHTLKVSLRK
                     SP"
     gene            5107..5904
                     /locus_tag="VEL68_22260"
     CDS             5107..5904
                     /locus_tag="VEL68_22260"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXZ38771.1"
                     /translation="MRRGRWKVVLFSYLWAGVFLFIYPTEGPAQPANTGGNTKQAEVT
                     YLQGRAEVLPKGEAAWKPLRVGSFLSMEDEGRTAEQSRIEIRLPDRSILRFDQKSTFK
                     MKNLFFDAQEGSREIQVEIPMGKTWANVRKAFGPKKVFEVASANAVAGVRDTVWRMNV
                     ATDRSTLIRVYEGTVEVYSPFVKPDYKPEEGGFKVPREVRGPQEVPRPYQEVSKEQWE
                     QIVLTQMMQVVVPAVGKPEAPTSFQPEEDQRDEWVRWNQQRDRQVRP"
     gene            5920..6462
                     /locus_tag="VEL68_22265"
     CDS             5920..6462
                     /locus_tag="VEL68_22265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXZ38772.1"
                     /translation="MLNIPNLFSIVRVVLLLPFIYFLQQKSYGLAFAILVLAGLTDAI
                     DGAMARILHQRTVLGAYLDPAADKLLMTFSYIALSSFGLLPWWLTFLVIGRDVVIVLG
                     VLILRLLSRPAEARPSAASKATTCLQIATIAAPLFSLSLYPSPWLTDGLTLATAVGTI
                     ISGLQYIGRGVNVLRKGAVE"
     gene            6588..6662
                     /locus_tag="VEL68_22270"
     tRNA            6588..6662
                     /locus_tag="VEL68_22270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6620..6622,aa:Val,seq:gac)
     gene            6774..8693
                     /gene="thrS"
                     /locus_tag="VEL68_22275"
     CDS             6774..8693
                     /gene="thrS"
                     /locus_tag="VEL68_22275"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXZ38773.1"
                     /translation="MEQIEVRFPDGTIRQHPRGVLLRDLLPKGAEADQTVAARLDGRV
                     MDLQAPLEQGGSLEWVSLSSDLGVEVLRHSTSHVMAQAVQSLFPEAKITIGPAIKEGF
                     YYDFDVSRGFSSEDLPRIESKMKEIVERNLPIIRRVVSREEAIRTFREKGEAYKVELI
                     EELPDRQVTLYQQGEFMDLCRGPHLPSTGNIRAFKLTSVAGAYWRGDERNKMLQRVYG
                     TAFPTAEALEKHLFLLEEARRRDHRKLGRELDLFSINEEAGAGLVIYHPKGALLRTLL
                     EDFEKREHLRRGYQIVIGPQILKLDLWKRSGHYDNYRDKMYFTEVEGQGYGIKPMNCL
                     AHMLIYKSRIRSYRDLPLRYFELGTVHRHEKSGELHGLLRVRGFTQDDAHILCTPEQL
                     NGEIKGILEFVRDVMKVFEFPYALEISTRPERGTIGSDEDWERATTALRSALDESRLS
                     YQINEGEGAFYGPKIDVKLQDALKREWQCATIQVDFAMPERFDLSYIGPDGEKHKPVM
                     VHRVILGAMERFIGVLTEHFAGAFPTWLAPVQVILLTVTDPQIPYAESVYRKMVEAGI
                     RVEKDFRNEKLGLKVREAEVQKIPYMAVIGEREVKQELIAPRARTGKTLNPMKIEEFI
                     GRIKEESRIGGESYR"
     gene            8677..9198
                     /gene="infC"
                     /locus_tag="VEL68_22280"
     CDS             8677..9198
                     /gene="infC"
                     /locus_tag="VEL68_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007069236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXZ38774.1"
                     /translation="MNRIAKETVGINWGIRAPEVRVIDGDGKQVGILPLKEAMKLAEE
                     QGLDLVEVAHNATPPVCRIMNYGKYKYQQNKRTHEARKHQTVIQVKEVKLRPRTEDHD
                     FQFKLRHAKRFLEEGNKVKISVLFRGREMAYPEFGKEILNRFIEGVKDLMVVEQPPRL
                     EGRNMVTILTPKS"
     gene            9318..9512
                     /gene="rpmI"
                     /locus_tag="VEL68_22285"
     CDS             9318..9512
                     /gene="rpmI"
                     /locus_tag="VEL68_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXZ38775.1"
                     /translation="MKLKTKRGAAKRFKMTGGGKVKRSKAYASHLLSSKTTKRKRKLR
                     KNVVVDKRDSRNVKRLIPYA"
     gene            9649..10005
                     /gene="rplT"
                     /locus_tag="VEL68_22290"
     CDS             9649..10005
                     /gene="rplT"
                     /locus_tag="VEL68_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXZ38776.1"
                     /translation="MPRVKKSVKTRARRKKILDAAKGYRGSRRRLYRIAADTVDRGRK
                     FAYRDRKAKKRTFRALWITRINAAVRSHDLTYGQLISGLAKANVEIDRKMLAEVAFTD
                     AVGFSKIVEIAKGQLG"
     gene            10019..11035
                     /gene="pheS"
                     /locus_tag="VEL68_22295"
     CDS             10019..11035
                     /gene="pheS"
                     /locus_tag="VEL68_22295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXZ38777.1"
                     /translation="MQDEIKRLEREALEELKGASGELQVLAVRTKYLGRKGLLTHLLR
                     GMGKIPADQRPLIGQLANQIKANLEGASDQALEKARSREQGERQVSEGIDFSLPGPKP
                     PRGGRHLITQVTEEIVAIFERLGFEVSEGPEVEMDYYNFEALNFPKDHPARDMQDTFY
                     FSPNIVLRTHTSPVQVRVMEKRNPPLRIIAPGKVYRHDSDPTHSPMFHQVEGFVVDQG
                     VTFADLKGTLTFFVHQLFGSGVQMRFRPSFFPFTEPSAEVDVQCVICQGDGCTVCKRS
                     GWLEILGSGMIDPEVFRFVGYDSEIFTGFAFGLGVERIAMLRVGLNDIRLFYENNIQF
                     LKQF"
     gene            11070..13475
                     /gene="pheT"
                     /locus_tag="VEL68_22300"
     CDS             11070..13475
                     /gene="pheT"
                     /locus_tag="VEL68_22300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXZ38778.1"
                     /translation="MKISLDWLKDYVDVTPSPAELADLLTHSGLEVESLQEWRPGFRG
                     VVVARLDSFRPMVESDHLSICSVHDGKRSYSIVCGAPNLKTGGRVALALEGGVLPGGQ
                     RIGRTQFQGVPSEGMLCSEKELGVSEEGDGIMYLGPDASLGVPLEEVLPLEDWVLDIN
                     ITPNRSDCLCLLGIARELAALTGKSLRFPEMKKIEVDPSTDRLTSVVIERADLCPRYV
                     AKLILGVQIAPSPFWMRRRLQVSGVRAINNIVDVTNYVMLEMGQPLHAFDFERLEEKR
                     IVVRTAARGQTFSTLDGTLRAMPGEALMICDGKKPVALAGIMGGLNSEVEPDTRNILL
                     ESAYFDPMGIRRTSKQLGLSTEASLRFERGIDANGSLCAAERAAGLMMALAGGKVARG
                     QVDAYPRRIEALNILLRVDRVNQVLGTSVGGKEIHGYLKSLQMEVREEKADSFLVTVP
                     TYRVDLTREIDLIEEVARLHGFHRVPVTLPSGRVAPEKKTPMQMGMAQARSLLAAFGF
                     WEVVTYSFISPQVLRDLQLEESEKRAKVLRIQNPLSEDQAVMRTTLVPGLLYTARSNI
                     YRQNLDLKLFELGRVFFPRGEDQLPEEVEYLGGILSGLREEESWARKRMEVNFFDLKG
                     VLEGVFEGLGVSGLEFVADPKSSFLHSSKACRICVKGEEIGVMGEVHPALRERFDLKQ
                     DVFLFEVDFQKVVERMTVRRVFVSLPRYPAVARDLAVVVDGGVAAGDILRTLWAANNG
                     WIKEIRLFDLYQGSPVPPGKKSLAFRLIYQKEDRTLTDREVNDFHQTLIQVLSRDYKG
                     ILR"
     gene            13534..13827
                     /locus_tag="VEL68_22305"
     CDS             13534..13827
                     /locus_tag="VEL68_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HXZ38779.1"
                     /translation="MTKADLVETIYEKIGFSRKESAEIVDLVFDLMKETLENGEKIKI
                     SGFGNFMVRQKRSRKGRNPQTGKEIQITARRVLSFKPSQVLRKALNVTPVTPS"
     gene            13870..14235
                     /locus_tag="VEL68_22310"
     CDS             13870..14235
                     /locus_tag="VEL68_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXZ38780.1"
                     /translation="MEKKSAPSIPEKLYYKIGEVSQITGVEPYVLRYWESEFKIINPV
                     RTNSKQRLFRKRDLELILEVKKLLYKEKFTIAGAKKKLQEIKGMREKQLKLDLPDRQY
                     YDGLLRVRKELQDLRGLLS"
     gene            14403..14479
                     /locus_tag="VEL68_22315"
     tRNA            14403..14479
                     /locus_tag="VEL68_22315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14437..14439,aa:Pro,seq:ggg)
     gene            14572..>15045
                     /locus_tag="VEL68_22320"
     CDS             14572..>15045
                     /locus_tag="VEL68_22320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38781.1"
                     /translation="MNQPSPSGVPGTPRRGIPRFILLLFFLSCLLVCACGETRVKRDL
                     TLARDFRPQEVQNLAVVNLDPQVQFSQFVEAELLKKGYRVKEGSTVGQFLKSEGLLKE
                     GSLDPPALAKIGSRLQVQGIVLCSVMEFSRFRDSYRLSIRCVAPETGNTLWYAEGG"
BASE COUNT         3558 a         3869 c         4485 g         3133 t
ORIGIN      
        1 tggtgagcac ccggtatctt tccgatggag cggtagaagt aaccgtggaa attcgaatgg
       61 ccagccagac cgctccgacc gattctttgg ccgctaccgt cttgccccct caagccatgg
      121 ggacccaatc ccagaatatc cccaaggcag ggaatccggt ttatacggga gtgatcttcg
      181 atgcccgggg cctaagcctc cgcccagcca tgtctcccaa ggtcttggat gaagatggaa
      241 gggaggttta tggctcggcc tttgtctccc aggaatgggc aacgaaattc ggaatcctgg
      301 gctatgtaaa ggacctggaa ggggccaaga agagtgaccg ggtagcggcc aatcccttgg
      361 tggtcaaagc ctcgaaagtt tcgggcaccg caggatgcga cttggtcatc agcaatgccg
      421 atgctcaggg aatgcgggac atgtccaaga atttgagctt cttggaacaa tgccgcgtgc
      481 tcatcgtggt ggactgaaga aataccagga tacaggatac gggagtcagg attcaggaga
      541 aagagcgggc ctgatctgac ttccggattc tggcttctga actcttatta taaggagggt
      601 ttcatgaaac ggcgacgtct cttgtattgg ctggcatttc tgacgatggt ggcgatgttg
      661 gccatgttga tctcttcctg cgcaaagcct acccgcaagc cagaagccgc gctggacacc
      721 ccggagcatc acgtctttac gggttacaag ctgtacgaca agggcgatta tgagggggcc
      781 cggagagagt tcgtcctggc catgcagctc gacaagaatt attcccccgc ctacgtcggg
      841 ctcggcctgt gcttcggctc ccagaatgat ttcaaagccg cctacgaaaa catgaagaag
      901 gccaaaagcc tggccaagaa gaaggaggat cgagtcgacg cccacgtagg catgatccgc
      961 ctgtactcca tggaacggaa ggataaatgg ctgaaggatg cggaagatga attcgaggat
     1021 gcggtgaaac ttgaccccaa atcctcggcc gcttatttct acatgggaaa ggcctacaaa
     1081 ttcgggtatg agttcgacaa ggcgggtacg caattcaaga aggtgctgga cctgaacgat
     1141 aagtacctgg tcgaagcgga caacgaatgg aaagtcgtgc agaagatcca gcgggcggcg
     1201 ccgggatcga tgatcgggaa aaagatcgcc ctgatcgaag agatcacccg ggccgacctg
     1261 gcggcgctct tcattcagga actcctcctg gagaaactct accagaagcg gacccccgcc
     1321 cagttcgaca ccagcttcca ggctcccacg gacacaagga agttcgaggc ggataagatc
     1381 gtaaaggccg agccggccac ggatattcag gcccacgttc tgcgggccga catcgaagcc
     1441 gttctccgcc tgggggtgcg tgggctggag cccaccccgg accacaagtt cctcccctat
     1501 gaaaaggtta accgggcggc ttatgccatg atgatcgaag acattctgat caaggtcacc
     1561 ggcaatcaaa aactggccac ggaatatatc ggctccaaat ctcctttccc ggacttgcgc
     1621 aacgacctgc cctatttcaa cgcggtgatg gtgaccacct cgcgcgggat catggaggcg
     1681 aaggatatga gcaccgggga attcggaccc cgcgggacgg tctctggagc ggatgctttg
     1741 ctgattatcc gcaagctgaa ggatgaactg cgcttcttct aagaaagggg tgaatttctt
     1801 gggcaagcat gtttggatcg ggggatggtt gatccttttc cttctttgcg ggagcctgcc
     1861 ggccttcccg cagcaggccc aggtggttac tgccgaagga gtgggggcgg tcattggggg
     1921 agaccgggcc atcgcgcggg accaggccat ccaggatgcc ctgcgtaagg ccgtggagca
     1981 ggccgtagga accctggtct cttccgagac gatggtccag aacttccaga ccctgagcga
     2041 caagatttac atccagtctc aggggtacat ccagaattac cagattgtct ccgaaacccc
     2101 cggtccgaac gtccaccaag tcacgattca ggcgaccgtg gccatgggaa acctggagca
     2161 ggacctccag gctctgggat tcctgctggg gcaggtgggg aaaccccgga tcatgatcct
     2221 ggtggccgaa cagcagatcg gccagcagta ctacaattat tggtggggga cccaccgagg
     2281 gacccagacc gatctcaatg tcgccgagaa tacgattatg gatcggttcc gggagaaagg
     2341 ttttgacatc gtggaccccc aggcccaggc cgggagtatc aagattcctc cagcctttca
     2401 ggtggtggaa ctctccaacc agaacgcgat taccatcggg aaacaggtcg acgctgaaat
     2461 cgtcattgtc ggcaaagcct tggcccgagc ggcgggaagc atagcaggaa cggccatgaa
     2521 gtcccttcag gcaaatattt ccatgcgggc catccaggta gacaacgccc gggtcctttc
     2581 ctcggggacc gaaaatgccg cggccgtcca tatcgacgaa gtgaccggcg ggtcggaggc
     2641 catcaagaag gccagcatca agatgtccga taagatgatg gacgatatac tcaagaattt
     2701 ccagaagagg gtgggggcta ccaccctggt tcagcttatg gtattcgggc tgtcgggcgc
     2761 ggacgatgtg aggaaattca agacctcggt cctgggccag gttcggggag tggaggggat
     2821 tcatgaacga agcttcgcgg aaaatacgct gaaggtggac gtggacattc ggggcaacgc
     2881 ccagtctctc tcccaggaga tcggccggaa gactttcccg gatttcaacg tgaaagtgat
     2941 tcgcagcacc tggaataccc tggaggttca agtctcccct aaatgagcaa tgcccaacgg
     3001 gcatttagca ataatcaatg gattataaca gacagaccaa gagcaaatgg tctacccgcc
     3061 tgaacagaaa ggagcgaatg aagggaatcg agagatttcg atggcctttt ctggtgagtg
     3121 tgacgatttt ttccctggcc tgcgctggag gggcacccca ctccgtggtt cccgattaca
     3181 aggatcgaat gccccgttcg gtcgccgttc taccggtttt gaatgaaacc gtaagtctca
     3241 aggccccaga ggtttttcgg cctattttgc tgaacaaact ttccatgaag gggtatgaga
     3301 ctcccgccct aggcttcatc gacggaaggt taagagagaa gggggtccgg gaggcaggcc
     3361 aggtgaacag cttgacccct caggagatcg gaaagctcct gggagtggat gctctgctct
     3421 acgcctccgt gacggagttt agcaccacct atcttctggc ttatgcttcc atgaccgtcg
     3481 gggcccggtt cgaactcaag gacaccaaga cgggtgagaa actctgggac tccgaccatc
     3541 aggtcaaaga aagcaaactg gggcttgacc agaaatcgat gggcgatacg atgcagtttg
     3601 ccgccggcca gtcctatgtt ccctatgccc agaaagtgat cgatgagagt ttcaaaaccc
     3661 tgcccaacgg cccccgggct tcggccccgg cccagacagg gtgtctcatg cctggtctat
     3721 aagatcatat ttccgaattc agaagggagg atagagtcat gttcttcaag gtatttcagg
     3781 tgtggagctt ggtgctcctt ctcctggcct cctgggggtg cgccagcatg ccccctgggg
     3841 gaggagggca ggcccaacag ccacagatgg cccaggccca gccggcagcg caacccaccg
     3901 tcccggctcg tcaagtcacc cataaaggcc cgaagctccg ggtggggatt gttgatttcg
     3961 tcaacaagag tagttacggg gcgggaagat tgggaacctc cgcctcggac atcctgacca
     4021 cggagctatt caagaccggc gccttcatca tgatagaacg ggacaggttg aagcaggttc
     4081 tcggcgaaca ggctctgggg cagaccggtg cggtgagcgc tgaaaccgct gcccaggcag
     4141 gaaaggtact gggcctcaac gccctggtga ccgggtccat ttcgcagttc ggagtgagca
     4201 ccggaggggc agactacgga gtctacaaac aaaaggtcca aaccgccaaa tgcaccgtgg
     4261 atgtgcgggt cgtcgatgct tcgaccgggc agctcttatt tgccgattca ggaaagggag
     4321 aatttgaaag gaaagcgcag gaagtcctgg gattgggtac ccgcgccggg tacgacgaaa
     4381 cgctgggaca ggaagctctt cgctcggcca tcaccaagtt tatggacaat ctggtgcaga
     4441 aactgcagag tgtggaatgg tcgggggccg tggccatggt gagcgggacc gatgtgtaca
     4501 tcaatgccgg gcgggatgtg ggattgaacc caggggatat tctgatggtg cagaccctgg
     4561 ggcaagaaat cgtcgatccc cagaccaaag tcgttctggg acggacccgg gggtcggtca
     4621 agggagaact cctggtcacg gagattgacg agaagtttac catctccaaa gtccggtcgg
     4681 gagtgggctt ccaagtaggc gacctagtga gaatcaagaa gtgagattgc agagagaggg
     4741 ggttatgagg aaattttcct tcctattgtt gttggggttg gccgtcttct gggggtgtgg
     4801 aatgccccag gagacgcgac ggggagtggg ccaggagggc tttctgctca ttcaagccgt
     4861 cccggacgat gccgaggtct atgtggacgg gcagttgatg ggaaaagcgg gaaagtatga
     4921 aaaggacccc ctggagttga gcggcggaac ccacaaaatt gaattccgca aggccgggtt
     4981 cctacccgag gtgagggaag tgtatgtggg gaaccagtcg cgccataccc taaaagtgag
     5041 cttgagaaaa tcgccttaga tcctccctga aagccagcgg gtctcgaccc aaagaaaggg
     5101 aaagttatgc gacgaggcag atggaaggtt gtgctctttt cttacctatg ggcgggagtc
     5161 tttctcttta tctacccaac cgaagggcca gcccaaccag ccaatacagg agggaacacc
     5221 aaacaagctg aagtcaccta cctccaggga agggctgaag tccttcccaa gggcgaggcg
     5281 gcttggaagc ccctccgggt ggggagcttc ctttcgatgg aagatgaggg ccggaccgca
     5341 gaacagtccc ggattgaaat ccgtcttcca gatcgttcga tcctgcgttt cgatcagaaa
     5401 agtaccttta agatgaagaa tctcttcttc gatgcccagg aaggctcgcg ggaaattcaa
     5461 gtagaaattc ccatgggaaa aacctgggcc aatgtgcgta aagccttcgg ccccaagaag
     5521 gtctttgaag tagcttcggc caatgctgtg gccggagtcc gtgacacggt gtggcgcatg
     5581 aacgtggcca ccgatcgatc caccctgatc cgggtgtatg aagggacggt ggaagtgtac
     5641 agtccctttg tcaagcctga ctacaagccg gaagaagggg gctttaaagt tccccgggag
     5701 gtgcggggac cgcaggaagt tccccgcccg taccaggagg tctccaagga acagtgggaa
     5761 cagattgtcc tcacccaaat gatgcaggtt gtggttcctg cggtggggaa acccgaagct
     5821 ccgacctcct ttcagccgga agaagatcaa cgcgacgaat gggtccgatg gaatcaacaa
     5881 cgggaccggc aggtccggcc ttaaaggccc cctttctgta tgctgaacat tcccaatctc
     5941 ttcagcatcg tccgggtggt tctcctcctt ccctttatct attttcttca gcaaaaaagc
     6001 tacggcttgg cctttgcgat cttagtactg gctgggctga ccgacgcaat cgatggagcc
     6061 atggcccgga ttctccacca gcgaacggtt ttgggagcct acctggaccc agcggcggac
     6121 aagctcctca tgaccttctc ctacattgcc ctgtcgagct ttggcctgct cccttggtgg
     6181 ttgaccttcc tggtaattgg ccgggatgtg gtcatcgttc tgggggttct tatccttcgc
     6241 ctgctctccc gccctgcaga ggcccggcct tccgcagcta gtaaggcgac cacctgttta
     6301 cagattgcca caatcgccgc tcccctattt tcgttatccc tatatccatc gccatggctg
     6361 accgacggat tgaccctagc gactgcggta gggacgatca tttcggggct gcagtacatt
     6421 ggaagagggg ttaatgtctt gcgaaaggga gccgttgaat agtctcatca gggaagatga
     6481 aaaggacccg ttattgatat accacatgaa taaaagcaga taagatgata ttttgcttga
     6541 caattttggg aggaattgat aagaataact atcttttaat aaaatgtagg cacgtagctc
     6601 agggggagag cgctaccttg acgcggtaga agtcaggggt tcaaatcccc tcgtgcctac
     6661 cagaaatcaa cttgaggcac ccaacaatct aatccccaaa gagaaaaggc atcatgcatc
     6721 gatgagatgg tgccttttct tattttcgcg cagcaggggt ttccggcttc ttcatggaac
     6781 agatcgaagt tcgttttccg gacggaacca tacgccagca cccccgggga gttctcctcc
     6841 gggaccttct gccgaaaggg gcggaggccg atcagacggt ggccgccagg ctggacggaa
     6901 gggtcatgga cctgcaagct cctctggaac agggaggctc cctggagtgg gtcagccttt
     6961 cctcggacct cggggtggag gtccttcgcc acagtacgtc ccatgtcatg gcccaggccg
     7021 tgcaaagcct cttccccgag gccaagatta ccatcgggcc ggcaataaag gaaggattct
     7081 attacgactt cgacgtctcc cgggggttct cttccgaaga ccttccccgt atcgaaagca
     7141 aaatgaagga aatcgtggag cggaatcttc ccatcatccg gcgggtggtc tcccgggaag
     7201 aagcgatccg caccttccgg gagaaagggg aggcctacaa ggtcgagctg atcgaagaat
     7261 tgcccgacag gcaggtaacc ctttatcagc aaggcgagtt tatggacctc tgccgcggcc
     7321 cccacctacc ctccacgggg aatatccggg ccttcaagct gaccagcgtg gccggtgcct
     7381 attggcgggg agatgagcga aacaagatgc tccagagggt ctacggcacc gcctttccca
     7441 cagcagaggc tctggagaaa cacctgttcc tcctggagga ggcccggcga agggaccacc
     7501 gcaagctggg acgggaactg gacctcttct ccatcaacga ggaggcgggg gctggtttgg
     7561 tgatctacca tcccaagggg gccctgctgc gcacccttct ggaggacttc gaaaagaggg
     7621 agcacttgcg gaggggctac cagatcgtca taggccctca aattctgaaa ttggaccttt
     7681 ggaaacggtc tggtcattac gacaattatc gcgataagat gtatttcacc gaagtggaag
     7741 ggcagggcta cggaatcaag cccatgaact gcctggctca tatgcttatc tacaagtcga
     7801 ggatccgcag ctatcgggac ctccccctcc gctactttga actgggtacg gttcaccgcc
     7861 acgaaaagtc gggggaactt cacgggctgc tccgggttcg agggttcacc caggatgatg
     7921 cccacatcct ctgcaccccc gaacagctca atggggagat caaggggatt ctggagtttg
     7981 ttcgggatgt gatgaaggtc ttcgaattcc cctatgccct ggaaatcagc acccgtccgg
     8041 aacggggaac catcggatcg gatgaggatt gggagcgggc tacgaccgcc ctgcgctcgg
     8101 ctctggatga atcccggctc tcctatcaga tcaatgaagg ggagggtgcc ttctacggtc
     8161 ccaagatcga cgtcaagctg caggatgccc tcaagcggga atggcagtgc gccacgatcc
     8221 aggttgactt tgccatgccc gagcgctttg acctttcgta tatcggcccg gatggagaaa
     8281 agcacaagcc ggtgatggtc caccgggtca ttttgggggc catggaacgt ttcatcgggg
     8341 tcttgaccga gcactttgcc ggcgcctttc ctacctggtt ggccccggtg caagttatcc
     8401 ttctgaccgt caccgatccg cagattccct atgccgaaag tgtctatcga aaaatggtgg
     8461 aagcgggtat tcgtgtggag aaggattttc gcaatgagaa actcggactg aaggtccgag
     8521 aagcggaagt gcagaagatt ccctacatgg cggtcatcgg ggaacgggaa gtaaagcagg
     8581 aattgattgc cccccgggcc cgcacgggaa agaccctgaa tcccatgaaa atcgaggagt
     8641 tcatcggaag gataaaggaa gaatcacgca tagggggtga atcgtatcgc taaagaaaca
     8701 gtgggcatca attggggcat tcgagctcca gaggtccggg tcattgatgg ggatgggaaa
     8761 caggtgggaa tcctgcccct caaggaggcc atgaagcttg ccgaggaaca ggggttggac
     8821 ctggtggagg tggcccacaa cgccacaccg cctgtgtgcc ggatcatgaa ttacgggaaa
     8881 tacaaatacc aacagaacaa gcggacccat gaagcccgga agcaccagac ggtcatccag
     8941 gtaaaggagg tcaaacttcg acctcgcacg gaggaccatg attttcaatt caagcttcgc
     9001 cacgcaaagc ggttcctgga agagggcaat aaggtcaaaa tctccgttct tttccgcggg
     9061 cgggagatgg cctatccgga attcgggaaa gaaattctga accgcttcat cgaaggggta
     9121 aaggatctga tggtggtgga acagcctccc cggctggagg ggaggaacat ggtcaccatc
     9181 ctcactccca agagttaagc ccttcaatga gcctagcgat tctttttttg atccagaaag
     9241 tgggggagaa agtacatttt cccatcaggc aaattcttta acttaatgta ttttgataaa
     9301 aaataagagg taatttaatg aaattaaaga caaaaagagg agctgcaaaa cgattcaaaa
     9361 tgaccggagg cggaaaggtc aaacgttcca aggcctatgc cagccatctt ctttccagca
     9421 agacaaccaa gcggaagaga aaacttcgca agaacgtcgt cgtggataaa agggattccc
     9481 ggaatgtaaa gaggctgatc ccttacgcgt aacaatattt gatttatcga acatggacat
     9541 aaataacatg gatacggagt ggcagagacg gataaaaggt tcaagtcctt tctgtgtttg
     9601 tctgccttca tctgcgtcca atatcggttt gtgaaggagt aacgaaacat gcccagagtc
     9661 aagaaaagtg tgaaaactcg cgccagaaga aagaaaatcc tggatgcggc caaagggtac
     9721 cgggggtccc ggcgtcgtct ttaccggatt gctgcggata cggtcgatcg aggccgaaag
     9781 tttgcttacc gggaccgcaa ggcaaagaag cgaacctttc gggcgctttg gatcacccgc
     9841 atcaacgccg cagtccgttc ccatgatctt acctacggcc agttgatttc gggcctggcc
     9901 aaagccaacg tggagatcga ccgcaaaatg ctggcggaag tggccttcac cgatgcggtg
     9961 ggcttttcca aaattgtgga gatcgccaaa gggcaattgg ggtgagggag acctcccaat
    10021 gcaggacgag atcaaacgcc tggaaagaga agcgctggag gaattgaagg gagcgagcgg
    10081 cgaactccag gtcctagcgg ttcggaccaa gtatctcggc cgaaaagggc tcttgaccca
    10141 tctcctccgg ggaatgggaa aaatcccggc tgaccagcgt ccgctcatcg gccagctggc
    10201 caaccagatt aaggcgaatt tggagggggc gtccgaccag gctttggaga aagcgcggtc
    10261 ccgcgagcaa ggggagcgtc aagtcagcga aggaattgat ttcagccttc caggaccgaa
    10321 gcccccgcga ggcggccggc acctgatcac ccaggtgacg gaggagatcg tagccatttt
    10381 tgaacgcctg gggttcgagg tatccgaagg ccccgaagtg gagatggatt attataattt
    10441 tgaggccctg aatttcccca aggaccatcc cgcccgggac atgcaggata ccttttattt
    10501 ctcccctaac atagtcctgc ggacccacac atcgccagta caggtgcggg tgatggaaaa
    10561 gcggaatccc cccctgcgga tcatcgctcc aggaaaggtc taccgccatg attccgaccc
    10621 aactcacagt cccatgttcc accaggttga aggctttgtg gtggaccaag gggtgacctt
    10681 tgcagacctc aaggggacgc tcactttttt cgtccaccag cttttcggct ccggggtgca
    10741 aatgcgcttc cgccccagct tttttccctt caccgagccg agcgccgagg tggacgttca
    10801 atgcgtcatc tgccaaggag acggatgcac ggtgtgcaaa cggagcggat ggctggagat
    10861 tctggggtcg gggatgatcg acccggaagt cttccgcttt gtggggtacg attccgaaat
    10921 cttcaccgga tttgccttcg gactgggagt ggaacggatc gccatgctcc gggttgggct
    10981 caacgatatt cgccttttct atgagaataa tattcaattt ttgaagcagt tctgatcgat
    11041 tctccctctc ggatcgagca agatagccca tgaagatcag tctcgattgg ttgaaagatt
    11101 atgtggatgt aaccccttcc ccggcggagc ttgctgacct tctgactcat tccggtttgg
    11161 aagtggaaag cctccaggag tggagaccgg ggtttcgagg ggtggtcgtg gcacgcctgg
    11221 attcgttccg ccccatggtg gagagcgacc atctgtcgat ctgttctgtc catgacggga
    11281 aaaggtccta ttccatcgtc tgtggcgctc ccaacctgaa aaccggggga cgggttgccc
    11341 tggcattgga ggggggggtc cttccggggg gccaaagaat cggaaggacc cagtttcagg
    11401 gagtaccgtc ggagggaatg ctctgttccg aaaaggaact gggggtgagc gaagaagggg
    11461 acggaatcat gtatttggga ccggatgctt ctctgggagt ccccctggaa gaagtccttc
    11521 ccctggaaga ctgggtcttg gatatcaaca ttaccccgaa ccgttccgat tgcctttgcc
    11581 tgttggggat tgctcgggaa ttggcggccc ttacggggaa aagcctccgc ttcccggaaa
    11641 tgaaaaaaat tgaagtggac ccttccacgg atcgattgac ctcggtggtt attgagcggg
    11701 cggacctttg ccccagatat gtcgccaaac tcatcctggg agttcagatc gccccctcgc
    11761 ctttttggat gcgccgaagg ttgcaggtct cgggggtccg ggcgattaac aacatcgtgg
    11821 atgtgaccaa ctatgtcatg ctggagatgg gccagcctct tcatgccttc gacttcgaac
    11881 ggctggagga gaaacggatc gtcgtccgga ctgcggcccg ggggcaaacc ttcagtactc
    11941 tggacggtac cctgcgggcg atgcccggag aggccttgat gatctgcgac ggaaaaaagc
    12001 ccgtcgcctt ggctggcatc atgggcggat tgaactcgga ggtcgagccc gacactcgca
    12061 atattctgtt ggagagcgcc tatttcgacc ccatgggcat ccgccgaacc tctaagcagc
    12121 tggggctgag caccgaagcc tccctgcgct tcgagcgggg gatcgatgcc aatgggtccc
    12181 tttgcgcggc tgaacgggcg gcaggcttga tgatggcttt ggcaggggga aaagtggccc
    12241 ggggacaagt ggatgcctac ccccgcagga tcgaagccct caatattctc ttgcgggtag
    12301 atcgggtgaa tcaagttctg gggacctcgg tcggcgggaa ggaaatccac gggtacctaa
    12361 agagtctcca gatggaagtc agggaagaaa aggccgattc cttcctggtc acggttccca
    12421 cttaccgggt ggatttgacc cgggagatcg acctgatcga agaagtggcc cggctccacg
    12481 ggttccatcg ggttcctgtt actctgccct cgggaagggt cgcccccgag aaaaagaccc
    12541 ccatgcagat gggaatggcg caagcgcgga gcctcttggc cgcctttggc ttttgggaag
    12601 tggtcaccta tagctttatt tctccccaag tcctgcggga cctgcaattg gaggaatcgg
    12661 agaagagagc aaaagtcctg cggattcaaa acccccttag cgaagaccag gcggtcatgc
    12721 gcacgaccct ggttccgggc ttgctttata cagcccgcag caacatttac aggcagaacc
    12781 tggacttgaa gctctttgaa ctgggtcggg tttttttccc gcggggggag gatcagttgc
    12841 ccgaagaagt ggaatacctg ggggggattc tatcgggcct acgggaggag gaatcttggg
    12901 cgagaaaaag aatggaagtc aacttcttcg acctcaaggg ggtcctggaa ggcgtctttg
    12961 aaggactggg agtttccggg ctggaatttg ttgccgatcc taaatcatcc ttcctgcact
    13021 cgtccaaagc ttgccggatt tgtgtaaaag gggaagagat cggggtcatg ggggaagttc
    13081 atccagccct gagggagcgt tttgacctaa aacaggacgt tttcctcttc gaggttgact
    13141 ttcaaaaggt agtcgagcgg atgaccgtgc ggcgcgtctt tgtttccctt ccccgctacc
    13201 cggccgtagc ccgcgatttg gccgtagtcg tcgatggggg agtggcggcg ggggacatcc
    13261 ttcggacgct ctgggcggcg aataatgggt ggatcaaaga aatccgcctc ttcgacctct
    13321 atcaggggag tccggttcct ccggggaaga agagccttgc cttccgcctg atctatcaaa
    13381 aggaggaccg gaccctcaca gaccgggaag tgaacgattt ccatcaaacc ctgattcagg
    13441 tcctcagccg ggattacaaa ggcatcctcc gttgagggga atcagctgag gaattttcat
    13501 ttcagattca taagatcaaa ggaggggaag atcatgacca aagcggatct cgtagaaacg
    13561 atttatgaga aaattggatt ctcccgcaag gaatcggcag agatcgtgga cttggttttc
    13621 gacctcatga aagagaccct ggagaatggg gagaaaatta aaatctccgg gttcggcaat
    13681 ttcatggtgc ggcagaaacg gtcccgcaag gggagaaatc cccaaaccgg caaggagatt
    13741 cagatcaccg cccgccgggt cttgagcttt aaacccagcc aagttctgcg caaagcgctg
    13801 aatgtaactc cggtcactcc ctcgtaatgc ggggagaaaa acctagaatc cccttggggg
    13861 tcgaaggtta tggagaaaaa gtccgctcct tcaattccgg aaaagttgta ctacaaaatc
    13921 ggagaagtca gccaaattac tggggtcgag ccctacgtgc tccggtactg ggagtcggaa
    13981 ttcaaaatca tcaaccccgt gaggaccaat tctaagcagc ggcttttccg gaaaagggac
    14041 ctggaactga tcctggaagt gaaaaaactc ctctataagg aaaaattcac catcgccgga
    14101 gccaaaaaga agctgcagga aatcaagggc atgcgtgaga aacagttgaa gttggacctc
    14161 ccggacagac aatactatga tggtcttctc cgggtcagga aggaattgca ggaccttcgc
    14221 ggcctcctca gttgaaaggc ggaatgagga atggggggcg aggaatcgcg gattgcggaa
    14281 ttgaaaagat tccgggcgaa acaggattca ttcaacttaa ggcacttgac gcactcaaga
    14341 tacgtgagac gctttttttg atcgttgaca gtcctttcgg ttaatgctat tttttagtca
    14401 accggggcgt agcgcagtct ggtagcgtac ctgaatgggg ttcaggtggt cgctggttca
    14461 aatccagtcg ccccgaccac ggatttcatt cccgcgaatg gcggaaggga ggggggcagg
    14521 agatttttcc tgcccccctg aatgcttttg gggagaggag aaatccattc catgaatcag
    14581 ccctctccat ccggagttcc gggtaccccg cgaaggggaa ttccccgatt cattttgctc
    14641 ctcttttttc tttcctgctt attggtctgt gcctgtggtg agactcgggt caaaagggac
    14701 ctgaccttgg cccgggattt ccggcctcag gaagtccaga acctcgccgt cgttaactta
    14761 gacccccagg ttcaattctc ccagtttgtg gaagcagaac tgctgaagaa aggatatcgg
    14821 gtaaaagagg gttcaactgt gggacagttc ttgaagagtg agggcctgct caaagaaggt
    14881 tcattggacc ccccggcctt ggccaagatt ggaagccgcc ttcaggtgca gggaattgtt
    14941 ctctgcagcg ttatggaatt cagccggttc cgggattcct accgactgag catccgttgc
    15001 gtggcacccg aaactgggaa taccctttgg tatgccgaag gggga
//
LOCUS       DASPRR010000606         5021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2085421, whole genome shotgun sequence.
ACCESSION   DASPRR010000606 DASPRR010000000
VERSION     DASPRR010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085421"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VEL68_22325"
     CDS             complement(<1..425)
                     /locus_tag="VEL68_22325"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXZ38782.1"
                     /translation="MKSGIGGRKIWVILVLLAVILLLGYGLGKFDLSARKPSKVECLG
                     QEELSAPVFSLPDLKGDRVDLFSFKGEVIIIEFWATWCGPCKEEIPVLSQLYKKYRDK
                     GLVVIGVSLDRKEPQEVKKFLDQLQIEYINVMGDEEVLE"
     gene            complement(786..1508)
                     /locus_tag="VEL68_22330"
     CDS             complement(786..1508)
                     /locus_tag="VEL68_22330"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXZ38783.1"
                     /translation="MDRLQILKKSFFFSSLSEPTLREISRLFNEEKYDREDYIYFEGD
                     LPEWLHIVLEGRVKLLKHSDTGKDIILQVYTPGDMFGEVSLFDRKPYSSSAQAMEPST
                     ILKLSRKDFFLFVGRHPFVSNEMIIDLGRQLREAHATIKSLAVDRVEQRIAHILLKLA
                     DKVGVSEKGGIMINLPLTRQDLADMAGTTVETAIRVMSRFTKSKFIKPANGKIFLLHP
                     QALQRVLEGKEDFESPATGKKA"
     gene            complement(1587..2432)
                     /gene="mutM"
                     /locus_tag="VEL68_22335"
     CDS             complement(1587..2432)
                     /gene="mutM"
                     /locus_tag="VEL68_22335"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXZ38784.1"
                     /translation="MPELPEVETVVRGLRNRIVGWELSQVEVCQEKCLGGRKEALAAL
                     LQKKKILAVERRGKNVILRLSRGGALFVHLGMSGRLRVLPRSAPREKHTHLVFSFLKH
                     GDQLRFIDPRRFGRVVWEGGKKGEWAFLSRLGPEPLEIPPSEFAARMRSRHREIKPLL
                     LDQHFLAGVGNIYADESLHRAGIHPRRKSDSLSGKTLSRLHRAVQGVLSEAIKARGTS
                     VRSYVDSSGSAGGYQHSLRVYGREGESCLACGVRIVREIVGGRSSFYCPRCQRKLSAG
                     RAGKQ"
     gene            complement(2538..3284)
                     /locus_tag="VEL68_22340"
     CDS             complement(2538..3284)
                     /locus_tag="VEL68_22340"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HXZ38785.1"
                     /translation="MRLEAAGGTPLRKSYLILLILGLVAVVVGGLVYLMIPGPRGGGD
                     FFNGIELHGNIHRLKTLILSFGLWAPLISAFLMVAQSVVLFLPAFPIFVVNALVFGPF
                     YGLLLSWSSAVTGSMACFSIAKTLGRPVVERFVKKDHLETADRALKKYEKYVILLFGF
                     VPVVSFDVISYAAGLTLLAYWEFLLLVCIAQVPSALFYTLLVHRIDRGTLDVYWIVAA
                     VLFFLLGFGSTAMRAYLNRRRQKESPESAP"
     gene            complement(3251..3502)
                     /locus_tag="VEL68_22345"
     CDS             complement(3251..3502)
                     /locus_tag="VEL68_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HXZ38786.1"
                     /translation="MKKWEYQITRYQMEDLSRGKDAEEKAFYCDQKGQCFLHDTSRAA
                     ADMIRDTLNEEGKKGWELVQFGYHRNEMLCVWKRPAEPL"
     gene            complement(3539..4048)
                     /locus_tag="VEL68_22350"
     CDS             complement(3539..4048)
                     /locus_tag="VEL68_22350"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXZ38787.1"
                     /translation="MGDRGRKDSRGLTGLEVLGVAIRAEAQAYRFYTQALKGVQNPLL
                     REKLSRLAAEEKRHRQVLEERYRRSSGEEFPPIPRKGGVEGKGKMPKDLSPEEVLKLA
                     IQKEQEAARFYQKEAQRSGDMSGRFMLEYLADFERNHERSLQAELKALTRFPEWFSIK
                     DPMVLLVGP"
     gene            complement(4249..4740)
                     /gene="tsaA"
                     /locus_tag="VEL68_22355"
     CDS             complement(4249..4740)
                     /gene="tsaA"
                     /locus_tag="VEL68_22355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HXZ38788.1"
                     /translation="MEQIILKPIGRVRNSIRAKKREGWESVVSRIELLPKYAGALDGI
                     EGYSHFLVLFWLSRVPPVARGKKLKIHPRSRPDLPLVGFFATRTQYRPNPVGLTQVQF
                     MGRKGNILVVKGLDALNGTPVLDIKPISPKTEMPTKFRVPDWYPRLWKKPSKKAGSRP
                     VRQ"
BASE COUNT         1100 a         1472 c         1246 g         1203 t
ORIGIN      
        1 ctctccaata cttcctcatc ccccatgacg ttaatatatt cgatttgaag ttgatccagg
       61 aatttcttga cttcctgcgg ttccttgcgg tcaagggaga ccccgatgac caccagtccc
      121 ttatctctgt atttcttgta aagctggctg agcaccggga tttcctcttt gcagggaccg
      181 caccaggttg cccaaaattc gataatgatc acctctccct taaaggaaaa gaggtccacc
      241 cggtccccct tcaggtccgg cagggagaaa acgggggcgg aaagctcttc ttgccccagg
      301 cattccacct tcgacggttt ccgcgctgaa agatcaaatt tgcccaaacc gtatcccagc
      361 aaaaggatca ctgccaacag tacaaggatt acccaaattt ttcttccacc aattccgctt
      421 ttcacctttt gattcctgtt tctcttttct ccagagccgt gccttgcgcc ttcctgggag
      481 aaaatcttca ctctgaattt acgagggaga aaagatcctc tcccggccga attcgtattt
      541 atgccatcgg cttccggcag aactggcgat tggccaacgg caaagaataa acagaagaaa
      601 ggcgggaatc ccttcagact ctcccgcctt tctccggaaa cgcgccttga ttatttgtag
      661 tattcctttt tcatcctgtc catgaccagc tggagtttgc ccacaggatc atttttgtga
      721 taatgacaca ggttgcagga atggaaatat tctatttcca atcctgcccc gaccaagggg
      781 aaaaatcagg ccttcttgcc tgtcgctgga gattcgaaat cttctttgcc ttccaggacc
      841 cgctgcaagg cctggggatg gaggagaaag atcttcccgt ttgccggctt gatgaatttg
      901 gacttggtaa accggctcat cacccggatc gccgtttcta ccgtggtccc cgccatatcg
      961 gccaggtcct gccgggttaa gggcaggtta atcatgatcc cccctttttc ggagacccct
     1021 accttatctg ccagtttcag aagaatatga gcgatccgct gttccacccg gtccacggcc
     1081 aggctcttga ttgtggcgtg ggcttcccgc agctgcctcc ccagatcgat gatcatctcg
     1141 ttggaaacaa acgggtgtcg gcccacaaag aggaagaaat ctttgcgcga gagtttcaaa
     1201 atggtggagg gttccatcgc ctgcgcggat gatgagtatg gctttcgatc aaaaagggag
     1261 acttctccga acatgtcgcc cggagtgtag acctggagga tgatgtcctt gcccgtgtcc
     1321 gagtgtttga ggagtttgac cctgccttcc aggacgatgt ggagccattc aggaaggtcc
     1381 ccctcgaaat agatatagtc ttcccggtca tatttttctt cgttgaagag gcggctgatt
     1441 tccctcaagg tgggctctga gagggaggag aagaaaaagg attttttcaa tatctgcagc
     1501 cggtccatcc gcccccgcca gtttttgaag ggaagtcgat ttcgttttca attttagaac
     1561 ggggtggagg attatgcaac atgattttat tgctttcctg cccgacccgc agaaagtttg
     1621 cgctggcacc gggggcaata gaagctgctc ctacccccca cgatctcccg cacgattctt
     1681 acgccgcagg ccagacagga ttccccctcg cgcccgtaaa cccgcagaga atgctggtat
     1741 ccccctgcgg acccgctgga atccacgtag gagcggaccg atgttcccct ggccttaatg
     1801 gcttcgctca acaccccctg caccgcccgg tgaagccggg acagggtctt cccggacaga
     1861 gaatccgact tcctccgggg atggattcct gcccggtgca gggattcgtc ggcatagatg
     1921 tttcctactc ctgccagaaa atgctggtcc aggaggagcg gtttgatttc tcgatgcctg
     1981 gatcgcatgc gggctgcgaa ttcagacggg gggatttcca ggggctctgg gccgagccga
     2041 gagaggaaag cccattctcc ctttttccct ccctcccaga cgactcgtcc aaaccggcgg
     2101 ggatcgatga atcggagctg gtccccgtgc ttcaggaacg agaagaccag gtgggtgtgc
     2161 ttttcgcggg gggcgctccg gggcaagact ctcagtctcc cgctcattcc caggtgcacg
     2221 aacagggcac cccccctgct cagacggagg atgacattct ttccgcgccg ctccacagcc
     2281 aggattttct ttttttgcag cagagcggcc agcgcctcct tgcgcccccc taggcatttt
     2341 tcctggcaga cctccacctg ggagagttcc cacccgacga tgcggttccg cagcccgcgc
     2401 acgaccgttt ccacttccgg caactcaggc atgtctcttc atttccttac atccccgatc
     2461 ttcatccccc cggtattttc ctttcccttc caactcccca cccatatatg aacaggtggt
     2521 tcgcaaatgc ggggcgttca aggggcggac tcgggtgact ccttctgccg gcgccgattc
     2581 aggtaggccc gcatcgccgt gcttccgaat cccaacagga aaaaaaggac tgcggcaaca
     2641 atccagtaga catccagggt tccccggtcg atccggtgca ccaggagggt gtagaacagg
     2701 gcgctgggga cctgggcgat gcagaccagg agaaggaact cccagtaagc taggagagtc
     2761 aggccagcgg catagctgat cacgtcaaaa gaaaccacgg ggacgaagcc aaagagaagg
     2821 atgacgtact tctcgtactt cttcagggcc cggtccgccg tctccaagtg atccttcttc
     2881 acgaaccgtt caacgaccgg ccgaccgagg gttttggcga tcgaaaaaca agccatggac
     2941 cccgtaaccg cgctgctcca ggagaggagg aggccgtaaa aggggccgaa aaccagagca
     3001 ttgaccacga agatgggaaa ggcggggaga aacaggacca ccgattgggc caccatcaag
     3061 aaagcagaaa tgaggggagc ccagaggcca aaggagagga ttagggtttt cagacggtgg
     3121 atattcccat ggagctcgat tccattaaag aaatctcccc cgccgcgggg acccgggatc
     3181 atgagataga cgagccctcc gacgactacc gccaccaagc caagaatgag gaggataagg
     3241 taggattttc tcaaaggggt tccgccggcc gcttccagac gcacagcatc tcattccggt
     3301 ggtatccaaa ctgaaccagt tcccacccct tcttcccctc ctcgttgagg gtatctcgga
     3361 tcatgtcagc cgccgcccgg gaggtgtcat ggagaaaaca ctgacccttc tggtcacagt
     3421 agaaggcttt ttcctccgcg tctttgcccc ggctcaaatc ctccatttgg taccgggtga
     3481 tctggtattc ccattttttc atggccactg ccctctttcg aatcagagcc cttccctctc
     3541 agggcccgac cagcaacacc atggggtctt tgatggaaaa ccattcggga aaacgggtca
     3601 gggctttcag ctccgcctgg agactgcgtt catgattccg ctcaaagtct gcgagatatt
     3661 ccagcatgaa gcggccgctc atatccccag acctctgcgc ctctttctgg tagaatcggg
     3721 ccgcctcctg ctctttttgg atggctaact tcaggacctc ttccgggctt aagtccttgg
     3781 gcattttccc ctttccctct acccctccct tgcggggaat cggcgggaac tcttctcccg
     3841 agcttctccg gtatctctct tccaggacct gccggtgcct tttttcttcc gcggcgaggc
     3901 gggaaagctt ttcccgcagc agcggattct gcaccccttt cagggcctgg gtgtagaaac
     3961 ggtacgcttg cgcctcggcc cggatggcca cccctaagac ctccagcccg gtcaatcctc
     4021 gagagtcctt ccgtcctctg tctcccatcg cactcaaacc tcccctaagc tataaaagac
     4081 ttctgctcct cggaataccg ccgtaccttt cccaggcctt cacctcttcg attttggtca
     4141 tcctcttctt tctcttttca gaaatcattc tgataagaat gatttcacct tcccatgata
     4201 tgtatcagca aatcctgtat cctcccctca ccttcccttt caggcctctt attggcggac
     4261 cggtcgactc cccgctttct tggagggttt cttccacagc cgaggatacc aatcagggac
     4321 ccgaaatttg gtcggcattt ccgtcttggg cgagatgggt ttgatatcca ggacaggggt
     4381 gccattcagt gcatccaaac ctttcacgac aagaatattc ccctttcttc ccatgaattg
     4441 gacttgggtg aggccaacgg gattgggacg atattgggtc cgagtagcaa aaaaccccac
     4501 taggggtagg tcagggcggg acctggggtg gattttcaat tttttccccc ttgccacggg
     4561 gggaacccga ctcaaccaga ataaaaccag aaaatgggag taaccttcga ttccatccag
     4621 ggcccccgcg tatttcggca ggagttcaat cctggaaacc acggattccc acccctccct
     4681 tttcttcgct cgaatggagt tacgaaccct gcctatgggc ttaagaatta tttgttccaa
     4741 tccaccccct attcctcata gctggagaga aaccttttct ttatgaaaag gcgagcggag
     4801 ggatgtttat ctattaattt ttgaatatat cagtcccatt ttttcttttc aacctaaacc
     4861 ttttttacca tcaccatccc gttgggaaag cgataaagcg tgggaaaaaa tcaagaactg
     4921 agttcccact gtacactttt tcctacacga ccgccactaa gacatcaaaa aaaatgcctg
     4981 ggaagtttat tcagctccca ggcatttcat taaaaaagtt g
//
LOCUS       DASPRR010000607         5152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1920198, whole genome shotgun sequence.
ACCESSION   DASPRR010000607 DASPRR010000000
VERSION     DASPRR010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920198"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VEL68_22360"
     CDS             <1..652
                     /locus_tag="VEL68_22360"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXZ38789.1"
                     /translation="RSHALRGLRALHRCLPEGSHRMKMDVVISGVGGQGNIFASLVIS
                     EYAMKKNLTVIGAETIGAAQRGGAVVSHIRISDGEIFSPIISRGQADLLIGMEYVELL
                     RHMRLLNPKGMYLLNAMKIPTVLNNMALDEYPATEDILKAIRKYCPHGYVVEASAAAK
                     KLGNIQMTNVVMLGALVKVAPFFEYNEMKLLVAANSPTRFKDANLKGFEEGHKLIQ"
     gene            724..1140
                     /gene="ndk"
                     /locus_tag="VEL68_22365"
     CDS             724..1140
                     /gene="ndk"
                     /locus_tag="VEL68_22365"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXZ38790.1"
                     /translation="MERTLSIIKPDGVKKGLIGEVVKRFEKAGLKVVAMKMIHMTKKA
                     AEGFYAVHRGKPFYNSLTDFMSSGPCVVLILEGEKAIGRNRELMGATNPKDAAAGTIR
                     KEYAANVEQNIVHGSDAPETAAFEMGYFFNALEICA"
     gene            1270..2520
                     /locus_tag="VEL68_22370"
     CDS             1270..2520
                     /locus_tag="VEL68_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXZ38791.1"
                     /translation="MPLNFNPDIIVIGGGASGLMAAGRAAELGAKVLLLEKMGRVGIK
                     LALTGKGRCNLTNGGDRQSFIENYRHNGKFLHNVFSRFFNQDLISFFHQKGLPTVEER
                     GKRVFPASNRAQDVVRVLREHVASCGVQTFLHTPARAILQENDRVVGVRTDSGVRAAS
                     RVILAPGGASYSRTGSTGDGYRMAQELGHTLMAIRPALVPLEAEEGYIRELQGLSLKN
                     VRATLFSSGRKAGEEFGEMLFTHFGVSGPIVLTLSGPAVEEFPRGRVELSIDFKPGLT
                     HEQVNQRLLREFRAGGRRKIINIMPNLLPQRLVPVLLRRGEISPELKGGEIHAGTRRR
                     MVDLLKDWRLTLRRARPLEEAIVTAGGVATKEIHPSTMESRIVRGLHFCGEVIDIDGK
                     TGGYNLQAAFSTGWAAGEAAAKAR"
     gene            2517..3584
                     /gene="rlmN"
                     /locus_tag="VEL68_22375"
     CDS             2517..3584
                     /gene="rlmN"
                     /locus_tag="VEL68_22375"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXZ38792.1"
                     /translation="MRTRNGRVALKDLSLRELEAFIVSLGEKPFRAIQVARWLYGKGV
                     GTFEEMTNLSRSFRQKLEEAAWISRLGSAEVHSSEDGTRKFRFILADGEAVESVLLPE
                     RDHLTLCLSSQVGCPLGCRFCLTGKRGWVRNLIASEIIDQVVGVRSTLSPGEKLTNLV
                     LMGMGEPLLNYQNVLQALEVLRSPLGFQFSNRRITLSTAGIIPEMVELLSRRNFVKLA
                     ISLSATTDEQRSSLMPINRKYPLKSLLSACREIPLPNRERITFEYVLIHGVNDSPEDA
                     RRLTHLLKGIKAKVNLIAFNEYPGSPFRRPSEEVVQNFREILMAKGFTAMLRQSKGSD
                     ILAACGQLGGRGNTKLPLNIA"
     gene            3696..3899
                     /locus_tag="VEL68_22380"
     CDS             3696..3899
                     /locus_tag="VEL68_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HXZ38793.1"
                     /translation="MKLTGRVKWFNDAKGYGFIEQEGGRDIFVHYTAIVGEGFKSLTE
                     GQKVEFEIIEGAKGPQATKVVKM"
     gene            4212..5048
                     /locus_tag="VEL68_22385"
     CDS             4212..5048
                     /locus_tag="VEL68_22385"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXZ38794.1"
                     /translation="MKIAGIQMNGSLDLKKNLEKAQLLAGLAAENGAQMVCFQNLFLY
                     SWFPAEINPENFRLAEELPGPTTEVMQEVARKNGIVLVLPLFEKDPRGAHFNTAVVID
                     ADGSLLGKYRKIHVPQLPLWEEKAYFQPGDLGFPVFATRYGKIGIQICWDNFFPEGTR
                     ILALKGAQLIFSPTAAALDSHQKWEKVICANAAASGVFVFRVNRVGKEAKQNFYGKSF
                     CATPEGDLVDQPSGAIEGVVLAAIDLSEIERTRRLWTFLRDRRPELYGELVNSSDSCI
                     VG"
BASE COUNT         1180 a         1356 c         1523 g         1093 t
ORIGIN      
        1 tcgatcccat gctctgcgtg ggctgcgcgc tctgcataga tgtctgccag aagggagcca
       61 tcgcatgaaa atggacgtgg ttatttcggg cgtgggaggc caggggaaca tctttgcctc
      121 tctggtcatc tctgagtacg ccatgaaaaa gaacctgacg gtaatcgggg cggagaccat
      181 cggcgcggcc cagcggggcg gggccgtggt ttctcacatc cgcatctcgg atggggagat
      241 tttttccccc atcatctccc gcgggcaggc cgacctgctc atcgggatgg aatacgtgga
      301 gctgctgcga cacatgcgtt tactgaaccc caagggaatg tacctcctga acgccatgaa
      361 gatccccaca gtgctgaaca acatggccct ggatgaatac ccagccaccg aggatatcct
      421 aaaggccatc cggaagtact gcccccatgg gtacgtggtg gaagcgtcgg ccgcggccaa
      481 aaaactgggg aacatccaga tgaccaacgt ggtcatgctg ggcgctctcg tcaaggtggc
      541 gcccttcttc gaatacaacg agatgaagct gctagtggcc gccaattccc ctacccggtt
      601 caaagatgcc aatctgaaag ggttcgagga aggacataaa ttgatccaat aacaatcggc
      661 tagatcctgt aggggtggtt tttagccccc ccctacggct gaccgccgtc tttgggaggt
      721 tccatggagc ggactttgtc gattatcaag cccgatgggg tcaaaaaagg cctgatcggt
      781 gaagttgtca aaaggtttga aaaggcgggc ctaaaagtgg tggccatgaa gatgattcat
      841 atgaccaaga aggcagcgga ggggttctac gccgttcacc gggggaagcc cttctacaac
      901 agtctgaccg atttcatgtc ctccggtccc tgcgtggttc taattctgga aggagaaaag
      961 gccattggcc ggaaccggga gctcatggga gcgaccaacc cgaaggacgc ggcggccggg
     1021 acgatccgca aggaatacgc tgctaacgtg gaacagaata ttgtccacgg gtcggatgct
     1081 ccggagacag ccgctttcga aatgggttat tttttcaacg ccctggaaat ctgcgcgtag
     1141 gaaaaatatt caacaccgag acacagagtt cacagagaat ttgaatataa aaataatcgg
     1201 aaaaatcaaa ataattttag ttttcttaag ggtaatgatt tctctgtatc ctctgtgcct
     1261 cggtggtgaa tgcctttgaa ttttaatcca gatattattg tcatcggagg aggggcttcg
     1321 gggctgatgg cggcgggaag ggccgcggaa ctcggagcca aggtcctcct cctggagaaa
     1381 atgggacgag tggggatcaa actggctctc acaggaaagg gccggtgcaa cctgaccaat
     1441 ggcggcgatc gccagtcctt catcgaaaac taccggcaca acggaaaatt cctccacaac
     1501 gtcttctccc gcttcttcaa ccaggacctg atctccttct ttcatcaaaa gggtcttccc
     1561 acggtggagg aaagaggaaa aagagtattt cccgcttcca accgggccca ggacgtggtc
     1621 cgagtcctaa gggagcatgt ggcctcctgc ggggtccaga ccttcctcca tacccccgcg
     1681 agagccattc ttcaggaaaa cgaccgggtc gtcggagtcc ggaccgattc aggggtccgg
     1741 gccgcatccc gggtaatcct ggccccggga ggagcctctt actcccgaac cggatcgacc
     1801 ggcgatggat accggatggc acaggagctg ggccataccc ttatggccat tcggcctgcc
     1861 ctggttccgc tggaagcgga ggaagggtac atccgcgaac tacagggcct cagcctcaag
     1921 aatgtccggg ccaccctctt ctcctccggc cgcaaagcag gagaagaatt cggggagatg
     1981 ctctttaccc atttcggggt ttccggcccc atcgtgctca ccctaagcgg cccggccgtg
     2041 gaggaattcc cccgaggaag ggtggagctc tccatcgact tcaaaccggg tcttacccat
     2101 gaacaggtaa atcagcgtct gctccgggaa tttcgggcgg gagggagaag aaaaattata
     2161 aatatcatgc ccaacctcct gccccaaagg ctggtccctg ttcttctgcg gcggggggaa
     2221 atttcgccgg agttgaaagg gggggaaatc catgccggga cgcgcagaag aatggtggac
     2281 ctgctgaagg attggcggct caccctgcgg agggccaggc ccctggagga ggccatcgtc
     2341 acggctggcg gcgtggcaac gaaggaaatc catccttcca ccatggaatc taggatcgtt
     2401 cgaggcctcc atttttgcgg agaggtgatt gacatcgacg gaaagaccgg cgggtataac
     2461 ctgcaggcgg ccttcagcac cgggtgggcg gcaggagagg cggcggccaa ggcgagatga
     2521 ggacaagaaa cgggcgggtg gccctcaagg atttatccct gagagagctg gaggctttca
     2581 tcgtctccct gggagagaag cctttccggg cgatccaggt ggcccggtgg ctctatggaa
     2641 agggagtcgg aacctttgag gaaatgacca acctgtcaag gtctttccgc cagaaactgg
     2701 aagaggcggc ctggatcagc cgcctaggct ccgcggaagt ccattcgtcg gaggatggca
     2761 cccgcaaatt ccggtttatc ctagccgacg gagaagcggt ggagagcgtc cttcttccgg
     2821 aaagggacca tttgaccctt tgcctttcca gccaggtcgg ctgcccctta ggctgccggt
     2881 tctgtctgac ggggaagcgg ggatgggtgc gaaatctaat agcttccgag atcatcgatc
     2941 aggtcgttgg ggttcgatcc accctctctc cgggggaaaa gctgaccaac ctggtcctca
     3001 tgggaatggg agagccgctg ctcaattacc agaatgtcct ccaggccttg gaggtcctgc
     3061 gttctccttt agggttccaa ttctccaacc ggcggatcac cctttccacc gcagggatca
     3121 tcccggagat ggtggaactt ctttcccgcc ggaacttcgt caagctggcc atttcgctta
     3181 gtgccactac ggatgaacag cgcagcagtc tcatgcccat aaaccgcaag tatcccttga
     3241 agagccttct ttccgcatgc cgggagattc ccctgccaaa ccgagaaagg atcacctttg
     3301 aatatgttct tatccatgga gtgaacgatt ccccggaaga cgccaggaga ttgacccatc
     3361 tgctcaaggg aatcaaggcc aaagtaaacc tgatcgcctt caacgagtac ccgggaagtc
     3421 cgttccgccg cccctcggag gaagtggtcc agaatttcag ggaaatcctc atggccaagg
     3481 gctttaccgc catgctccgg cagagcaagg gctcggatat cctggcggcg tgcgggcagc
     3541 tggggggaag gggaaatacg aaattgccct taaatatcgc ttgacagggt tcagccattt
     3601 gtggtaatgc caacgtcgaa aagccgaagg gatgtcggcg ggcaaggggc cgataccgcg
     3661 ggtcgtcggc ccgggtgcgg aaaaggagga tttgaatgaa gttgacgggt cgggtgaagt
     3721 ggttcaatga cgcaaaaggg tatgggttta tcgaacagga gggtggtcga gacattttcg
     3781 tgcattatac ggccattgtg ggagaagggt tcaagtcact aacggaaggg cagaaggtag
     3841 aattcgagat catcgaaggc gccaaagggc cgcaggccac aaaagttgtg aaaatgtaat
     3901 tcggctttca ttttcagacc cgcattcagc cccggaagag ccccttccgg ggtttttttt
     3961 aatgcggaat gccgaatttg gaatgtggaa ttcaaccatc ctttatcggg atggccgttt
     4021 ttttcattga gacccatttt atcgcatccg agggaattgg aggtcgctct aagttctctc
     4081 gattctattt ttctcgatgc tgtccccttc cagcgcaggt ttttcattcc gcatgggtct
     4141 tttcctcctt gacccttggg tcgaaagggg ctaaactttt ttacaaaaaa agcaggaatc
     4201 gaggccctct tgtgaaaatt gccggcatcc agatgaatgg ttctctggac ctcaagaaaa
     4261 acctggaaaa ggctcagctg ctggcagggc tggcggcgga gaatggagcc caaatggttt
     4321 gcttccagaa cctcttcctg tattcctggt ttcccgcgga aatcaacccg gagaattttc
     4381 ggctggccga agaactcccg ggcccgacaa ccgaggtcat gcaggaagtt gcccgaaaga
     4441 atggaatcgt cctggttctt cccctctttg aaaaagaccc acgcggggcc cactttaata
     4501 cggcggtggt gatcgacgcc gacggcagcc tcctggggaa ataccgcaaa atccatgttc
     4561 cccagctgcc cctctgggag gagaaggcgt acttccagcc cggcgaccta gggtttccgg
     4621 tttttgccac tcggtacggg aagatcggga ttcaaatctg ttgggataat ttcttccccg
     4681 agggaacccg gatcctggcg ttgaaggggg cccagttgat tttctctcct acggccgcag
     4741 ctctggattc ccaccagaaa tgggagaagg tcatctgcgc caacgcggct gccagcggag
     4801 ttttcgtctt ccgggtcaac cgggtgggta aggaagccaa gcagaatttt tatgggaaaa
     4861 gcttctgcgc cactccggaa ggggacctgg tggaccagcc cagcggagcg atcgagggag
     4921 tggtcttggc ggctatcgac ctttcggaga tcgagcggac ccggcggctt tggacttttc
     4981 tgagggaccg gcgacccgag ctttacgggg aattagtcaa ttcgtcggat agttgcatag
     5041 tcggataatc agttgtcttc tcaactgaga ctattcgact ttccgcctga gcgaccaagc
     5101 gactatgcga ccatgggact aagtgactac caatgaacga agtaaccagg gc
//
LOCUS       DASPRR010000608        10279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_958748, whole genome shotgun sequence.
ACCESSION   DASPRR010000608 DASPRR010000000
VERSION     DASPRR010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958748"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VEL68_22390"
     CDS             <1..257
                     /locus_tag="VEL68_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HXZ38795.1"
                     /translation="SNLMMAGWEGAKILEEANHLLSACRQARGSLILVGNEVGMGIVP
                     ENAQARLFRDLSGFIQQKVAREADEVYFMVSGLPVKIKGS"
     gene            336..1421
                     /gene="cobT"
                     /locus_tag="VEL68_22395"
     CDS             336..1421
                     /gene="cobT"
                     /locus_tag="VEL68_22395"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HXZ38796.1"
                     /translation="MEGLKEMGLEKELGERLATIQPLKKEFFDQAQKRLDQLTKPRGS
                     LGQLEEIAKRYVAIVENARPLIRHKIIYTFAGDHGVSAEGVSAYPKDVTPQMVYNFIR
                     RGAAINALASHAKADVVVVDIGVDHLFEPMEGLVIRKVARGTGNIARGPAMSREEALR
                     AISVGWEMADHAMGRKADLVGTGDMGIGNTTPSSAILAAMTGLPVNQVTHRGTGIDDL
                     GLVRKIKIIEKALEVNSPDPQDPLDVLAKVGGLEIAGIAGLIIGCAGKRIPVVVDGFI
                     STAGAMIAVALNPRVREYLFAAHKSVEVGHQFMWEYIGQKPILDLSLRLGEGTGAALA
                     MSIIEASIKVFNEMATFAEAGVSEEKK"
     gene            1418..2170
                     /gene="cobS"
                     /locus_tag="VEL68_22400"
     CDS             1418..2170
                     /gene="cobS"
                     /locus_tag="VEL68_22400"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HXZ38797.1"
                     /translation="MTSFLLAWQFLTLFPGGKKDAEATPEVLGRSMAYYPLVGLLIGS
                     ILWAAYWVVSHAFPRTLCDGLAILLLVIITGALHLDGLADTLDGMAAGKSAEERLRIM
                     RDHRVGTFGAVGLVLVLGIKFLALNSLPEEIIGKTLIAALALSRWSMVQLTYRAPYAR
                     PEGGLGKVFKENVTRREMALATAFSLTIAVFLLRFWGAVLWLAVGVSALGIQLLFQKK
                     IGGITGDILGAANETHEVLVLVMVAGIFHGFF"
     gene            2353..2949
                     /locus_tag="VEL68_22405"
     CDS             2353..2949
                     /locus_tag="VEL68_22405"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXZ38798.1"
                     /translation="MEPDQTRLYLTRHGEVVNHGVYNGQNDVDITPKAIQQMQRLQEV
                     LKDKNLKGVYCSDLVRTQKGAEIIGQPHGLSPEAFPEFREVNFGRWQGLSYAQVMERY
                     PTDIPQWLRDVEGFRIPEGESMVDVRRRAIPKLQELIDRHRGEEILLVCHGALNRVIL
                     AEALHLPMAHLLRIEQNYGCLNIIEYTPTWTVVKLMNG"
     gene            3172..4071
                     /locus_tag="VEL68_22410"
     CDS             3172..4071
                     /locus_tag="VEL68_22410"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HXZ38799.1"
                     /translation="MAILVIPLLFVMGESAPAATFTDEVGRTIEVNGPPQRIVSVAPN
                     VTEILFALGLEDRLVGVSVYCQYPPEALKKEKIGGYMNPSLEKIVALRPDLVIGIAEG
                     DLRNFVDKLASLKVPVYITNPQDALEVLTSIRKIGEVTFAQEPARRIVRSMEERIRKI
                     QDKVRGRPRPRVLHILDFNPLISAGKGTFVDDLIRLAGGWNVAETAAGKYPHFSMEEV
                     LVQDPEVILLASMKSQDPLVKQRRWWERWKTISAVKQGRIYVLDSDLIHRPSPRMPDG
                     LEQVAKAIHPEVFKSQIPTPKSQ"
     gene            4129..5175
                     /locus_tag="VEL68_22415"
     CDS             4129..5175
                     /locus_tag="VEL68_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXZ38800.1"
                     /translation="MSRWTFKRWIFWMMILALLLAGVMGVSVSLGAVTIPLSRSWSIL
                     GHQFLGFSSSGKWTASDSSIILDLRLPRIISGALVGSALAMAGSVLQALLRNPLADPF
                     VLGISSGAAVGAVLAILFGLGTTFLGAYAVPGSAFGGALFTLFLVYFIARVEGRAPMN
                     TMLLAGVIVSSFFSAVIMFLISATSDEQIYNVTFWLMGNLEYMASQSLGIIFLYLMVG
                     GGILLALARDFNLLALGEETASELGVDVERVKKTAFIFASLITGAVVSVSGLIGFVGL
                     IVPHVVRMIWGPDHRFLLPASALVGAMLMVVADTLARTVMAPTEIPVGVVTALGGAPF
                     FVYLLRKKGHAVYH"
     gene            5159..6421
                     /locus_tag="VEL68_22420"
     CDS             5159..6421
                     /locus_tag="VEL68_22420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38801.1"
                     /translation="MPSIIKMEAVDFRYPERPIFSGLSLALEEGEVLGLIGPNSSGKT
                     TLLKLMDGLLRPQRGRVLLEERNLDQIPRAKVARTIAVVPQTMEVPFSFTVAEIVLMG
                     RAPYLTRFGWEKQKDLDVAREATALTGVAGLESRTFWELSQGEKQRVLIARALAQEPR
                     VMLLDEPTSHLDINHQVEINELIRRLNLQKGLTVLHISHDLNLAAEYCHRIVLLHQGS
                     VFSAGVPSEVITEENIRRVYETKVLVEKNPLSGAPRITLLGKGKIEKKGPQRAVHIIC
                     GEGSGVDSARRLLLRGYRVSLGVLNTGDTDQKIGKTLGLPMALDGPFSSISERGMEEN
                     WKLIEEADLVLLERFHVGPGNLANLRAALEALGKGKKVIVLENNLEYDFTGGEARAYY
                     QRLKERGAVFIPDHSRLLEEAEKYLNQN"
     regulatory      6473..6773
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6805..8655
                     /locus_tag="VEL68_22425"
     CDS             6805..8655
                     /locus_tag="VEL68_22425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXZ38802.1"
                     /translation="MKRFGRFRVLLFGLAAAIGVGPEATGAEVELGQVVVTSTRTEVE
                     ISDSPQSISVITKEEIMSTPDRTIPEVIQRTTGVLITNNGPTGSITTANIRGSEAGQV
                     LIMIDGRRINDAQNGLFDLSNLPLSKDEIERIEVLRGGASALYGADAMGGVVNIITKS
                     PTKEPYTRVDASYGRFATQEYSLTHRWKPGPLGYGLFLSRGLSDGFRPNSDYSAWILG
                     GDLSYDLPWKGELKVSARNIQKEVGTPGTITFTDPDDRQKDNLTQLDLNYRDKIGSAV
                     TLNFRGWQNISRQTFDQGTQGPDVGPPLLHKSYQTGGDLQATAALGSAHLVTGGVEGI
                     QDRVDSSSFGVQEGTRGAFYLQDEIEVVKAVTTTLGLRYDMNSLFTNQLDPRAALLVR
                     LPWDTRVRASVARSYRAPTFNDLFWPASAFTAGNPNLQPEKAWSYELGGEKKFSDLAI
                     FKVAGFYRDVTDLIHWAPDANFVWRPTNVQTAHIWGVETELVLSPQKGLSFPLNYSYL
                     YPRDQSTGEPISNKPKHIFNAGVEYFTSFGLKSSLKGRYVRYYVNNISTLNKDYFVVD
                     ARLGYEFKVYQNFKGEGFLSLTNAFDREYQVNEGYPMPPRSLTGGVSLFF"
     gene            8740..9636
                     /locus_tag="VEL68_22430"
     CDS             8740..9636
                     /locus_tag="VEL68_22430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXZ38803.1"
                     /translation="MKRFFLISILFHLVALLLLFSWEIPLTNRLLPRSVVQVSLVERI
                     EGKSEGKGEGGDSQPEQPPLKRKDVQEKKDEGVILQLARRIAQAAEEKKMEEPKQEKV
                     ESAQKEMKEEKPGAERAPEAEARTEETPAIQAGLAPLGGGGGASETVFSPQGFPTSGE
                     RWDGGVTFLASSGIEPGREGIPLGGGAKGSGAGQGEGGTFRLSSVSGSSGHGDSAIAE
                     IMRRIEKAKRYPRAARKMGTEGQATVRFRIKTDGKVEGVELLESSGSEILDQASLETV
                     QRAAPFPYKEGWLKVVIVFKIL"
     gene            9780..10061
                     /locus_tag="VEL68_22435"
     CDS             9780..10061
                     /locus_tag="VEL68_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38804.1"
                     /translation="MEKDWVFEDIEVESYSGYKGEESPRAFVHLGKRFEVLEILDRWY
                     EGGLDPKALRHDYFRVKTREGETFMLRYTPRFQAWTLCRRLPAPRVSNN"
BASE COUNT         2275 a         2680 c         3171 g         2153 t
ORIGIN      
        1 tctccaacct catgatggcc ggctgggaag gggccaagat tctggaggaa gcgaatcatc
       61 tgcttagcgc ttgccggcag gccagaggtt cgctgattct ggtcggcaac gaggtgggga
      121 tgggaatcgt tcccgagaat gcccaggcca ggctcttccg ggacctcagc gggtttatcc
      181 aacagaaagt ggcccgggaa gcggatgagg tctatttcat ggtgagcgga ctgccggtaa
      241 agattaaagg ctcatagaac agagtgcata gggcaaagat caatggtcaa tgaccaatgc
      301 gcaatgggca atgagtaaat aggaaggcaa tcttaatgga aggcctgaag gaaatgggat
      361 tggaaaaaga actgggagaa aggctggcaa cgattcagcc gctgaagaag gaattcttcg
      421 atcaagccca gaaacgtctg gatcaactga ccaagccccg gggaagcctg ggacagttgg
      481 aagagattgc caaacgctac gtagccattg tggagaatgc ccgtcccctg atcaggcata
      541 agatcattta cacctttgcc ggggatcacg gagtatcggc cgaaggagtc agcgcgtatc
      601 ccaaagacgt caccccgcag atggtttata acttcatccg caggggagcg gccatcaatg
      661 ccctggcgag tcatgcgaaa gcggatgtag tcgtggtcga catcggtgtg gatcatctct
      721 tcgaacccat ggaaggcctg gtcatccgca aagtggcccg gggaacggga aacatcgcca
      781 gggggccggc catgtcccgt gaagaggcgc tgcgggcgat ttccgtggga tgggaaatgg
      841 cggatcacgc catgggccgg aaagccgact tggttggaac aggagacatg ggaataggga
      901 acacaacccc cagcagcgcc atcctggcgg ccatgaccgg ccttcccgtg aaccaggtga
      961 cccaccgggg aacggggatc gatgaccttg gcctggtgcg gaagatcaag attattgaaa
     1021 aagccctgga ggtcaattcc ccggaccccc aagaccccct ggatgtactg gccaaggtgg
     1081 gaggcttgga aattgcggga atcgccgggt tgatcatcgg gtgcgcggga aaacgcattc
     1141 cggtcgttgt ggacggcttt atctccaccg ccggggccat gattgcagtg gccctcaatc
     1201 cccgggtccg cgagtacctc ttcgccgccc acaaatccgt agaagtcgga caccagttta
     1261 tgtgggagta catcgggcag aaacccatct tggacctgtc tttgcgtttg ggagaaggga
     1321 cgggcgcggc cttggccatg tccatcatcg aagcgtccat aaaagttttt aacgagatgg
     1381 ccacgtttgc cgaagcggga gtatcggaag agaaaaaatg accagttttt tgctggcctg
     1441 gcagttttta acccttttcc ccggagggaa gaaggacgcc gaggcaaccc ccgaggttct
     1501 cggtcggtcc atggcttatt atcccctggt gggcctcctg atcgggtcca ttctttgggc
     1561 agcctactgg gtcgtctccc atgcatttcc ccggacgctg tgcgatgggc tggcgattct
     1621 tcttctggtg attatcaccg gggcgcttca tctggacgga ctggccgaca cgctggatgg
     1681 gatggcggcc gggaaatcgg ccgaggagag actccggatc atgagggatc accgggtggg
     1741 aacttttggg gcggtggggc tcgtcttggt cctgggaatc aagtttttgg ccttgaattc
     1801 ccttcccgag gagattatcg gcaagacctt gatcgccgcc cttgccctga gccgctggtc
     1861 catggttcag ctgacttacc gggcacccta tgcccgtccg gaagggggcc tggggaaagt
     1921 cttcaaagaa aacgtgacga ggcgggaaat ggccctggcc acagctttct ctttgaccat
     1981 tgccgtgttt ctcctgagat tctggggagc ggtcctctgg ctggccgtag gagtttccgc
     2041 cctgggaatt caactcttgt tccagaagaa aatcggaggg attactggag acatcctggg
     2101 ggcggccaat gagacccatg aggttctggt cctggtgatg gttgcgggaa tctttcatgg
     2161 cttcttttag gcgatcccag aattgcaggg ccgattcatg tcggagactc gactctacga
     2221 gaatcgcctc taagaacaat ggtaatttaa aatgaggccg tgcatagtct gtcgctagcg
     2281 atatttaaga agatggggac taggaacagg tttcaaacct gcccctacgt tcttcccaaa
     2341 agaggataag aaatggaacc ggatcagacg cgactctatc tcacccgcca cggggaagtg
     2401 gtaaatcacg gagtatacaa cggccaaaac gacgtggata ttacccccaa agccatccaa
     2461 caaatgcaga ggctccagga ggtattgaag gacaagaatt tgaaaggcgt ttactgcagc
     2521 gatctggtgc gtacccagaa aggggccgag atcatcggcc aaccccatgg cctttcccct
     2581 gaggccttcc cggagtttcg ggaggtgaat ttcggccgat ggcaaggctt aagttatgcc
     2641 caagtgatgg agcgatatcc cacggatatt ccccagtggt tgagggacgt ggaaggcttc
     2701 cgcattcccg agggtgaaag catggtcgat gtgcgccggc gggcgattcc caaattgcag
     2761 gaattgatcg accgacaccg gggagaggag atcctgctag tctgccatgg agcgctgaac
     2821 cgggtaattc tggccgaggc tctccacttg cccatggccc accttctgcg gatcgaacag
     2881 aattatgggt gtctgaatat aatcgaatac acgcccacgt ggacggtggt gaaattgatg
     2941 aatgggtagc aagaatgcgc cctgaaccat ggcaggttca gggttgccct gcactcaacg
     3001 tagcacaggg cggaatgcag attgcagggg gcgcggcgac tcggctgtct tcgaccctga
     3061 ggctcagacc cgaagggagc tcgtcgacgt cgcgcgcctt tgagaaagtc gccttctgta
     3121 gaggcgcccc gccgggtcgc ccctacgggt taagagagca tggaaaggct tttggccatt
     3181 cttgtaattc ctctcctttt cgttatggga gagagtgcgc ctgctgccac cttcaccgat
     3241 gaagtagggc ggacgataga agtcaacgga ccgcctcagc ggatcgtatc cgtggccccc
     3301 aacgtgacgg aaatcctttt tgctctgggc ctcgaagacc ggttggtggg agtaagtgtt
     3361 tactgccagt accctccgga ggctctgaag aaagagaaga ttggagggta catgaatccc
     3421 tccctggaaa agattgtggc cctgcgcccg gacctggtca tcggaatcgc cgaaggggac
     3481 ctgaggaatt tcgtggataa actagccagc ttaaaggtcc cggtgtacat taccaacccc
     3541 caggacgccc tcgaggtcct gacctccatc cggaaaatcg gagaagtcac ctttgcccag
     3601 gagcctgccc ggagaatcgt ccggtccatg gaggaaagga tccggaaaat ccaggacaag
     3661 gtccgggggc ggccccggcc ccgggtgctc cacatcctgg atttcaaccc cctgatcagc
     3721 gccggcaaag gaaccttcgt ggatgatctg atccgccttg ccgggggctg gaacgtggcg
     3781 gagacggccg cgggcaagta tccgcacttc agcatggagg aggtcctggt gcaagaccca
     3841 gaggtgattc ttctggcctc tatgaaatcc caggaccctc tggtcaagca acggcgttgg
     3901 tgggaacgat ggaagacgat atccgcggtc aaacagggcc ggatttacgt tctcgactca
     3961 gacctcatcc atcggccttc cccgaggatg ccggatggat tggaacaggt agcaaaggcc
     4021 atacatcccg aggtgtttaa atcccaaatc ccaaccccca aatcccaatg agattgaaaa
     4081 attcaacctt taatttggaa tttggtgctt agtgcttgga atttcaacat gtcccgttgg
     4141 accttcaaaa gatggatttt ctggatgatg atcctggctc ttctgctggc cggggtcatg
     4201 ggggtctcgg tttctctggg agcagtgacc atcccccttt ccagaagctg gtccattctg
     4261 ggccatcagt tcctggggtt ttccagttcc gggaaatgga cggcgagcga ttcctcgatc
     4321 atcctcgacc tgcggctccc gcggattatt tccggggccc tggtgggttc tgccctggcc
     4381 atggcggggt ctgttctcca ggccctattg cggaatcccc tggcggaccc ctttgtcctg
     4441 gggatctcta gcggagcggc tgtgggtgcc gtgctggcga ttctctttgg gttggggacc
     4501 acctttttag gggcttatgc ggtcccgggg tccgcatttg ggggcgcttt gtttaccctg
     4561 ttcctggtct atttcatcgc ccgcgtggag ggccgggcgc cgatgaatac catgctgctg
     4621 gctggagtca tcgtgagctc ctttttctca gcggtcatca tgtttttaat ctccgccacc
     4681 agcgacgagc agatctacaa tgtgaccttc tggctcatgg gcaacctgga atacatggct
     4741 tcccaatccc tggggattat cttcctttat ttgatggtgg gcggaggaat cctcttggcc
     4801 ctggcccgcg atttcaatct cctggccctg ggagaagaga ccgcgtccga gttgggagtg
     4861 gatgtggaaa gggtgaagaa gacggctttc atctttgctt cgctgataac cggggcggtc
     4921 gtttccgtga gcgggctgat cgggtttgtg ggcttgattg tcccccatgt ggtgcggatg
     4981 atctggggcc cggaccaccg gttcctcctc ccggcctcag ccctggtggg ggccatgctc
     5041 atggtcgtgg ccgacaccct tgcccggacg gtcatggctc cgacggagat tcccgtagga
     5101 gtcgtcaccg ccttgggggg ggcccccttc ttcgtttacc tcctgaggaa aaagggccat
     5161 gccgtctatc attaaaatgg aggcggttga ttttcgttac ccggaaaggc cgatcttttc
     5221 ggggttgagc cttgcgctgg aagaggggga ggtcttgggc ctcatcgggc ccaatagctc
     5281 gggaaagacg accctcctga aattaatgga tggactgctc cggcctcagc ggggaagggt
     5341 cttactcgaa gagaggaact tggaccagat tccccgagcg aaggtagccc gaaccatcgc
     5401 cgtggttcct cagaccatgg aggttccgtt ttccttcact gttgcggaga tcgtcctcat
     5461 gggaagggcc ccgtacctga cccggttcgg ttgggaaaag cagaaagacc tggatgtggc
     5521 ccgtgaggcc acggcgctca caggcgtggc cggcctggag agccggactt tctgggaact
     5581 gagccagggg gagaagcaga gggtgctcat cgctcgagct ctggcccaag aacccagggt
     5641 catgctgctc gacgagccca cttcccacct ggacatcaat catcaggtgg agatcaacga
     5701 attgatccga aggctgaatc tgcagaaggg actcacggtc ctccatattt cccatgattt
     5761 gaaccttgcg gcggagtact gccatcggat tgtcttgctt catcaggggt ccgtcttctc
     5821 cgcaggcgtt ccttcggagg tgatcaccga ggaaaacatc cgccgggttt acgagaccaa
     5881 agttttggtc gaaaagaacc ctctttccgg ggccccgcgg atcaccctgc tcggcaaggg
     5941 gaaaatagaa aaaaaggggc cgcagagggc tgtgcatatt atctgtggag aagggagcgg
     6001 ggtggattcg gcccgccggc ttctcctgag ggggtaccga gtctctctgg gagtcctgaa
     6061 tacgggggac acggaccaga agatcggcaa gacccttgga ctgcccatgg ccctggatgg
     6121 acctttttcg tccatctccg agcgggggat ggaggagaat tggaaattga tcgaagaagc
     6181 ggacctggtg ctgttggaaa ggtttcatgt gggcccgggg aacctggcca atctccgggc
     6241 ggccctggag gctctgggaa aagggaaaaa ggtgatcgtc ctggagaaca acctggaata
     6301 cgattttacc gggggagagg caagggccta ttaccagcgc ctgaaggaga ggggagcggt
     6361 attcattccc gatcattccc ggctgctgga agaagcggag aagtatttga atcaaaacta
     6421 ggctggccat aaaggcggtg ttcatccggt tgaataaccg gggccgagag tccccggaaa
     6481 ttgaacggag gttgtgaaag gctggacccg cagggttcaa gggaaggtgg tgcaagtccg
     6541 ccgctgcccc gcaactgtga ggggaacgaa agccacaaaa aagacaggtt agaggttgga
     6601 ggttagaggt ttgaggttaa aagaggaaat acctgctttt tatctttgtc tttgcctcta
     6661 acctcttacc tccaacctct aacctatctt tccgccactg gactccagtc cgggaaggcg
     6721 tggcgagtag gaagccccga gccagtagac ctgaccctga gggaaacact ttctgacctt
     6781 tcgcgggaaa ggagaaaatg ggtgatgaag aggtttggga ggtttagggt tttgcttttt
     6841 ggcctcgcgg ccgctattgg ggtgggtcca gaggccacgg gagcggaggt agaattaggc
     6901 caagtggtcg ttacaagtac gaggaccgag gtggaaatca gcgactcccc gcagtccatc
     6961 agcgtcatca ccaaggaaga gatcatgagc acccccgacc ggaccattcc tgaggtcatc
     7021 cagcggacaa ccggcgtgtt gatcaccaac aacggaccga caggatcgat taccacggcc
     7081 aatattcggg gctcagaagc cgggcaagtg ctgatcatga tcgacggccg ccggatcaac
     7141 gatgcccaga atggcctgtt tgacctcagc aaccttcccc tttccaagga cgaaatcgag
     7201 cgtatcgaag tgctccgcgg gggtgcttcc gccctatacg gtgccgatgc catgggcggg
     7261 gtggtcaata tcatcaccaa gtccccgacg aaggaaccct acacccgagt tgatgcctcc
     7321 tacggccggt tcgccaccca ggagtactcc ctgacccacc gctggaagcc gggtcccctc
     7381 ggttacggct tatttttgtc cagggggtta tcggatgggt ttcgcccgaa cagcgattat
     7441 agcgcctgga ttctcggggg agacttgagt tatgacttgc cctggaaagg ggaactcaag
     7501 gtttccgcca ggaacatcca gaaggaagtc ggcacgccgg ggaccatcac cttcacggac
     7561 ccggacgacc gccagaagga taatctgacc caactcgact tgaattaccg ggacaaaatt
     7621 gggtcggccg tgaccctgaa ttttcgggga tggcagaata tctcccgcca gacctttgac
     7681 cagggaaccc agggacccga cgtggggcct ccgcttctgc ataagagtta tcagacgggg
     7741 ggggacctcc aggcgacggc ggccctcggg agcgcccacc tggttaccgg cggtgtggag
     7801 gggatccagg atcgcgtgga cagcagctcc ttcggcgttc aggagggtac gaggggcgcc
     7861 ttctacctgc aggacgaaat cgaagtggtc aaggcggtca ccacgaccct cggcctgcgc
     7921 tacgatatga actcgctttt cacgaatcag ctggacccgc gggccgccct cctggtccgt
     7981 ttgccctggg acacgcgggt ccgggcctcg gtggcgcggt cttaccgtgc gcctactttc
     8041 aatgacctat tctggccagc cagcgccttt acagcgggta atcccaacct gcagcccgag
     8101 aaggcctggt cctatgaact gggaggagag aagaaattca gcgacctggc gatcttcaaa
     8161 gtcgcgggtt tttatcggga tgtgacagac ctcattcatt gggcgccgga cgctaacttc
     8221 gtgtggcgtc ctaccaacgt tcagacggcc catatctggg gcgtggaaac cgaactggtc
     8281 ctttctcccc aaaagggcct ttcgttcccg ctgaactatt cctatctcta tccccgggat
     8341 cagtcaaccg gcgaaccgat tagcaataag ccgaaacata tcttcaatgc cggggtagag
     8401 tattttacct ccttcggcct caaatccagc ctgaaaggaa ggtatgtgcg gtattatgtg
     8461 aataacatct ccaccctgaa caaggattat tttgtggtcg atgcccgcct ggggtatgaa
     8521 ttcaaggtct accagaattt taaaggggaa gggtttctca gcctgaccaa cgcatttgac
     8581 cgtgagtacc aggtcaacga ggggtatccc atgccgccgc gttcgcttac cggaggggtc
     8641 agccttttct tctagggggc ccgaggaaag aggtgcgggg ggactgagga agaaggttcg
     8701 attcttcctc gaaccatgtg tctcgtgtgt gtatttaata tgaaacgctt ctttcttata
     8761 tccatcctat tccatctggt cgccctgctg ctcctctttt cttgggagat tccgctgacc
     8821 aatcgtcttc ttcccaggag cgtggtgcag gtgtcgctgg ttgagcgaat cgaagggaag
     8881 tcagagggaa aaggagaagg gggggatagc cagccggagc agcctcctct gaaaaggaaa
     8941 gatgtccagg agaagaagga tgaaggggtg atccttcagc ttgcccgtag gatcgcccaa
     9001 gcggcggaag aaaagaagat ggaggaaccc aaacaggaga aggtcgagtc agcccagaag
     9061 gaaatgaagg aagaaaagcc cggcgcagaa agagctccgg aggcggaggc gagaacggaa
     9121 gagaccccgg ccatccaggc cggactggcc ccgctaggcg ggggcggggg agcgagtgaa
     9181 acagtgttct ccccgcaagg cttcccaacc tccggggaaa gatgggatgg gggggtgacc
     9241 ttcctggctt cgtcagggat tgaaccgggc agggaaggga tccctctggg aggcggcgca
     9301 aaaggttccg gggcgggaca gggcgaaggg gggacctttc gcttaagcag tgtttctggg
     9361 tcgtcgggcc atggagattc ggccatcgcc gagatcatgc gccggatcga aaaggccaag
     9421 cgttaccccc gggcggcccg aaagatggga acagagggcc aggctacggt ccgtttcaga
     9481 atcaaaaccg atggaaaggt ggaaggggtg gaactcctgg aatcatcggg ctcggaaatt
     9541 ctcgaccagg catccctcga gacggtccag cgcgccgccc cctttcccta caaagaagga
     9601 tggctgaagg tggtcatcgt ctttaaaata ctgtaaaagg cgttcaccac ggaggcacag
     9661 agatcacaga gaaatgaaaa ttgaattatc ccgggtcgaa aaaacgaaaa aggaaatctg
     9721 cttttttgcc aaaatttatt gcgtctctgt gtcctctgtg tctctgtggt gcaaaaagta
     9781 tggaaaaaga ctgggttttt gaagacatcg aagttgaatc ctattccggg tacaaaggcg
     9841 aagagagccc gagggctttt gttcatctgg ggaagcgatt cgaggttcta gaaatcctgg
     9901 accggtggta tgaaggggga ttggacccga aagcattgcg acacgattat ttccgggtga
     9961 agacgagaga aggagagacc tttatgctcc ggtacacgcc ccgcttccag gcctggaccc
    10021 tctgccgccg ccttccggcg ccgcgggttt cgaacaactg agggcaaatg ggacgcagat
    10081 gaacgccaga ttttcaggac tcgatctcag gcacaaaagg ggccgagggg gagtcatgac
    10141 tttctgcccc cgatgacttc tgcagaagtc gagcgaagcg tgtggggaaa gggctcaccc
    10201 agccgatgct ttctgcgtgc aatgttctta cccccgggcc acgacccagg catcctggat
    10261 atttcgaact ttgtgactc
//
LOCUS       DASPRR010000609        12813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2245401, whole genome shotgun sequence.
ACCESSION   DASPRR010000609 DASPRR010000000
VERSION     DASPRR010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245401"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VEL68_22440"
     CDS             <1..1551
                     /locus_tag="VEL68_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ38805.1"
                     /translation="RLKMSVLLIHPPVSKPCEPPPGIARLCGALQANEVAAAVLDMNL
                     GGLLALMEGVRDPSDTWTRRAVRNRTRQLDSLRNPAGYSNFSTYKRAILDSNRLLQVF
                     GNSRGLNLSLANSQRNSHSPVRSSDLIRAAEDPEEDPFFPYFEEILPSALETQTPRVV
                     GFSLNYLSQAVSTFSMIGFLRKRFPRLAVVLGGGLVTSWVRRPGWQNPFGGLVDSLVA
                     GPGESHLLSLFGKNSSQVKIHGLPDFDDLPLTGYLSPGLILPYSASGGCYWNRCLFCP
                     ERAEGNPYKPAAAAQVVQDLKDLTRRHRPVLIHLLDNALSPALLIQLAQDCLRVPWYG
                     FARITDHLADLDFCRALKKSGCVMLQVGIESGDPGVLEKLQKGIDLEVASRVLKNLRK
                     AGIATYVYLLFGTPAESEKEARKTLHFAARHREEIGFLNLALFNLPAYGEEAQTLATR
                     AFYEGDLSLYSTFIHPRGWNRDAVRSFLDKEFRKHPAIAPILRREPPLFTSNHAPFFV
                     EGMIKNLV"
     gene            1640..2413
                     /locus_tag="VEL68_22445"
     CDS             1640..2413
                     /locus_tag="VEL68_22445"
                     /inference="COORDINATES: protein motif:HMM:NF020475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HXZ38806.1"
                     /translation="MPKKETKVKYSKHIPALKVPARRRPPSCDTCSVMACWPKGPEDI
                     LKGPATCPTKNYPDLIKKAKDMYIKDPFHRKMQKAGARLEGMSSQTPPGGREINMKLT
                     RVEELVMFCKMMGYKKIGFAHCIGCIGEARMLSEIFRARGFETVQVNCKVGAIEKGEI
                     GISEDEKVRMFTFETICNNLAQALIMNHEKTDLNVIVGLCLGHDISFARASKAPVTTL
                     IVKDRRLGHNPALALYQGYPPCNFYYGRLGKATSETGGR"
     gene            2425..2706
                     /locus_tag="VEL68_22450"
     CDS             2425..2706
                     /locus_tag="VEL68_22450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38807.1"
                     /translation="MERKGKEKEISGWYVQKEQTPEEKAAQVKSVGDWYEKKHRLNYR
                     TVPLEIPGKMMLALGKMALERGMDFSELIEEILADYLRGKRIHWKAPKG"
     gene            2797..3117
                     /locus_tag="VEL68_22455"
     CDS             2797..3117
                     /locus_tag="VEL68_22455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HXZ38808.1"
                     /translation="MAKRHPLKITVIKKLSSKEVYGHSLPEVSETMAAYCDRLETGQE
                     FQVDESGAMPAGFCTWAWHDIFPAVTGLRFGGNYPWMKKEGMVIACCSDGARPVFFRI
                     ERVG"
     gene            complement(3284..4063)
                     /gene="lgt"
                     /locus_tag="VEL68_22460"
                     /pseudo
     CDS             complement(3284..4063)
                     /gene="lgt"
                     /locus_tag="VEL68_22460"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
     gene            complement(4138..4614)
                     /gene="lspA"
                     /locus_tag="VEL68_22465"
     CDS             complement(4138..4614)
                     /gene="lspA"
                     /locus_tag="VEL68_22465"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXZ38809.1"
                     /translation="MTLISGGVLVLDQCTKVLVEKTMTLHQSVEIIPNFAHLTYLRNT
                     GAAFGFLAGTRSSLRIALFALISSVAIGCIVYLIRSLRQHQKTLLVSLALILGGAIGN
                     LIDRLRLGEVIDFVDLHWYDTHWPAFNVADSAISIGVALLFIQMLRKGSLTFGEKD"
     gene            complement(4632..7439)
                     /gene="ileS"
                     /locus_tag="VEL68_22470"
     CDS             complement(4632..7439)
                     /gene="ileS"
                     /locus_tag="VEL68_22470"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXZ38810.1"
                     /translation="MDYKDTLNLPKTAFPMKANLPKLEPEILKKWEEGKIYQKLRQIA
                     KDRPKYILHDGPPYANGHIHIGTALNKILKDLIVKSRNMAGFNGEYVPGWDCHGLPIE
                     HEVDKSLGSKKGSYPISEKRKLCREFATRFIDIQRQEFKRLGVMGEWENPYLTMSYDY
                     EATITREFGRFVGKGSVYRGKKPVYWCAVCQTALAEAEVEYEDHKSPSITVKFPAISD
                     FGDRFPALKNKKVFVLIWTTTPWTIPANLAIAFHPDYKYVAAEVEGEVWILAEALLEQ
                     AMAQAEKKKYKVLEKFSGKEVEGLKCRHPFLDRESLLILATYITLDTGTGCVHTAPGH
                     GQEDYESGLEYNLPIYSPVDDQGRFTPEVKFFAGQYVFDANDAVIQKLKEVGALIYSE
                     VYTHQYPNCWRSKNPIIFRATEQWFISMEKNGFRQKALEAIDKVKWIPHWGHDRIYGM
                     IQNRPDWCISRQRAWGVPIVAFTCTACGEVLTEEPLVEYVSKIFEKGGADAWFDLPVK
                     ELLPPGTRCPKCRGEDFKKETDILDVWFDSGVSYAAVCEKRPNLKSPADMYLEGSDQH
                     RGWFHSSLLASVGTRGQAPYNSVLTHGFVVDGEGRKMSKSLGNVIVPDEVIKRYGAEV
                     LRLWVAAEDYRDDIRISEEILSRLSDAYRRIRNTCRFLLGNLHDFDPKKDSVPDSSLQ
                     DLDRWVLLRLQKLNSRLREAYENFDFHVVFHGIHNFCSVDLSALYLDILKDRLYTSPA
                     NSPQRRAAQTALYKVLDALIRLMAVILSFTAEEVWQQIPGAKERAKSVHLTLFPEVES
                     RYFDPKLEERFELLLKVRSEVSRVLEAARQDKLIGNSLEASVTLSGTGKILPFLRENE
                     AILKELFIVSEVLLSPTAAPGAQPSKEMEGLNILVSRAGGQKCPRCWVYDPKVGEGNG
                     YSPICPRCEAALAAIQAEGKS"
     gene            8038..9483
                     /locus_tag="VEL68_22475"
     CDS             8038..9483
                     /locus_tag="VEL68_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HXZ38811.1"
                     /translation="MAQQGLFKTEDWWAVWLGMLVFILSLFYLAGADLLGWVGRPMMW
                     TDFSKSFGPISKSYSGLGGFGSLLVTYIAFLVALTIGAKAIGFKIGKFIYGFTIIFWL
                     TILCVVLGNYANIAANSPGDIKRFGLNWSLRLTGESGLIIALLVGLFISNFLPRFAET
                     LKEAARPEWYIKTAIVILGAGIGVKAVGAMGLATAVMFRGLCAIIEAYLIYWALVYWM
                     ARKWFGFSREWSAPLASGISICGVSASIATGGAIRARPIIPIMVSSLVVIFAVVELLL
                     LPFLAQTFLYQEPLVAGAWMGLAVKTDGAAVASGAIVDALIKAKALTAQGINYEPGWM
                     LSTATTVKIFIDIFIGVWAFILAVIWTTVIEAQPGVKVRAIEIWNRFPKFIIGYALTF
                     VIMLVICFNNPGIMKNAVPASGEADVFRTTFFALTFFSIGLVSNFRKLWEEGIGRLAV
                     VYLVALFGFIIWIGLLISWIFFHGVKPPIIG"
     gene            9509..9670
                     /locus_tag="VEL68_22480"
     CDS             9509..9670
                     /locus_tag="VEL68_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38812.1"
                     /translation="MAEKKELVEELKKMKVEPFLPVEKKLVLWSIILGVVLLGFLVWV
                     SYTFFPAGH"
     gene            10124..>12813
                     /locus_tag="VEL68_22485"
     CDS             10124..>12813
                     /locus_tag="VEL68_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXZ38813.1"
                     /translation="MDVKECHCLPVDHLTSELETHLERGLTQKEAQARLQKHGPNELT
                     EKPREGFLKMLLDQFNNFLVIILIIAAVVSLLLGEIIDATAIMVIVILNAIVGVIQES
                     KAEQALAALKKMSAPNAQVIRDGHQMTIPSRELVPGDLVLVEAGNYIPADMRLVESVN
                     LKVEEASLTGESVPVEKVAGVVLDKEIPIGDRKNSAFMSTMATYGRGKGLVTGTGMHT
                     QIGMIAEMLQSYEDEATPLQQKLDQLAKTLGIICLAVCGIIFVYGLIRDTHFGTIMNE
                     GFIAYLRAEKKDIIELFMTAVSLAIAAVPEGLPAVVTICLALGMQSMIRRHALIRKLP
                     AVETLGCATVICSDKTGTLTQNEMTVVQAWAGGKSFKISGEGYNPVGQFSLEGKSFDA
                     AKDPNASLLLHAAMLCNDAKLEEGSEGKGEKAWRMVGDPTEGAMVVAAAKAGLWRKEM
                     EKALPRMQEIPFDSERKRMTTIHQQQTDSVNFYFDCPSNVAFVKGAPDIVLDLCDHII
                     MDGKTIPLSEEMKRNVLAANQDMARQALRVLGAAYRPMDGIPNQCRWEDIEKGLTFIG
                     LMGMIDPARPEAKEAVKLARGAGLKSVMVTGDYKDTAEAVAKEIGLLTPGGRVLSGAE
                     LDKMSDEALTGMIDKVDAFCRVSPQHKTRIVDALKAKGHVVAMTGDGVNDAPALKRAN
                     IGVAMGITGTDVSKETADMVLTDDNFASIVSAIEEGRIIYSNIRKFVYYLISCNIGEI
                     LIIFLAMLAGAPLPLRPVQLLMLNLVTDGAPALALGMEKGEPDIMKRPPRPTKEPVIN
                     WEMRIGTLVQAVVMTAAVLSAYFWALRVYPQQLERAQTIAFVSLCLSELLRAFTARSE
                     RYGIFSIGVFSNKWMLWADGSSLLLVLLVVYVPFLRPFFDT"
BASE COUNT         2935 a         3407 c         3597 g         2874 t
ORIGIN      
        1 aggttaaaaa tgagtgtgct cttgatccat cctcccgtca gcaaaccctg tgaaccaccc
       61 ccgggaattg ccaggctttg cggggccctt caggccaatg aagtggcagc cgctgtcctg
      121 gatatgaacc ttggggggct cctggccttg atggaagggg tgcgggaccc atccgacacc
      181 tggacccgca gggcggtccg aaaccgaacc cgccaactgg attccctgag gaaccccgcg
      241 ggttattcta atttctccac ctacaaaaga gccattctgg attcaaaccg ccttctccag
      301 gtctttggca actccagggg tttgaaccta agcctggcca attcccaaag gaattcccac
      361 tctccggtca ggtcctcaga tctgattcgg gcggcggaag atcctgaaga ggatcctttc
      421 ttcccatact tcgaggagat tctcccttct gctctggaga cccaaacccc ccgggtagtc
      481 ggcttttccc tgaattacct aagccaggca gtcagtacct tctccatgat tgggtttttg
      541 aggaagagat ttccaagact cgccgtcgtg ctcggaggag gactggtcac ttcctgggtt
      601 cggaggccgg ggtggcagaa tccatttggg ggactcgtcg attctttggt tgcggggcct
      661 ggagaaagtc atttattgtc tctttttgga aaaaattctt ctcaggtcaa aatccatggg
      721 ctgcctgatt ttgatgacct tcccctcacc gggtaccttt cccccggtct tattctaccc
      781 tattctgcat ccgggggatg ctattggaac cgatgccttt tctgcccgga aagagcggaa
      841 ggaaatcctt ataagcccgc agctgcggcc caggtcgttc aggatttgaa ggacctgacc
      901 aggagacatc ggccggtcct gattcatctc ttggacaatg ctctttcgcc ggcgctgcta
      961 atccagctgg cccaggattg cctccgtgtc ccctggtatg ggtttgcccg gattaccgat
     1021 catctggctg accttgattt ttgccgcgcg ttgaaaaaat caggatgcgt gatgctccag
     1081 gtggggatag agtcggggga cccaggagtt cttgaaaagc tgcaaaaagg gatcgacctg
     1141 gaggttgctt cgcgagtgct gaagaatttg agaaaagcgg gaattgccac ctacgtctac
     1201 ctccttttcg ggacccccgc agagagtgag aaggaagctc gcaagaccct ccattttgcc
     1261 gctcgccata gggaggaaat cgggttcttg aacttggcgc tcttcaacct gccggcttac
     1321 ggggaagaag ctcagacgct ggccacccgg gctttctacg agggggacct ttccctctat
     1381 tcgactttca tccatccccg agggtggaac cgggacgcgg tccgatcctt cttggacaaa
     1441 gaattcagaa agcacccggc cattgcccca atcctgcgaa gagaaccccc cctattcacc
     1501 tccaaccatg ctcccttctt cgtcgaagga atgataaaga accttgtcta aggacccccc
     1561 tttctgctat attccaacat cctttgattc gtcctctcct aaaagcctgc cggacccacc
     1621 aaaagaaagc gaggttgcca tgcccaaaaa ggagaccaaa gtcaagtact ccaagcatat
     1681 tccagccctg aaagttccgg ccagacgccg gcctcccagc tgcgacacct gctcggttat
     1741 ggcctgctgg ccaaaaggcc cggaggacat cctaaaagga ccggccacct gccccaccaa
     1801 aaattacccg gatttgatca agaaagcgaa agacatgtat ataaaggacc ccttccacag
     1861 gaagatgcag aaagcgggag cgcggctgga ggggatgtct tcgcagactc ccccaggggg
     1921 aagggagatc aacatgaagt tgacccgggt ggaggagctg gtcatgttct gcaagatgat
     1981 ggggtataaa aaaatcggtt ttgcccactg catcgggtgc atcggcgaag ccaggatgct
     2041 gagtgaaatc ttccgcgccc ggggattcga gaccgtacag gtcaactgta aggtgggggc
     2101 cattgaaaaa ggggaaatcg gaatcagcga agacgagaag gtccgcatgt tcacttttga
     2161 aaccatctgc aacaaccttg cccaagccct gattatgaat catgaaaaga ccgacctcaa
     2221 tgtgattgtc ggcctttgtc tcgggcatga tatctccttc gcccgagcct ccaaggcccc
     2281 ggttaccact ttgatcgtga aggaccggcg cttggggcat aatccggccc tggcccttta
     2341 ccaggggtac ccgccctgca atttctatta tggtcgactg gggaaggcaa cttcagagac
     2401 cggcggacgg taggagggag cgaaatggag cgcaagggga aagagaagga aatttccgga
     2461 tggtatgttc agaaggaaca gacccccgaa gaaaaggcgg cccaggtgaa gtccgtaggg
     2521 gattggtatg aaaagaagca ccgtctcaac taccgcaccg tccccctgga aattcctgga
     2581 aaaatgatgc tggccctggg aaagatggcc ctggaaaggg gaatggattt ctctgaactc
     2641 atcgaagaaa ttctggcaga ctatttgcgg gggaaaagga tccattggaa agcgccgaaa
     2701 ggatgattcc gcaatttgaa cacagggaag atcctttagg cgcttgatca cacttgagca
     2761 catccggggc acttttaatc ccaaaggagg gaaaacatgg ccaagagaca tcccttgaag
     2821 atcaccgtga tcaagaagct gagttccaaa gaggtttacg ggcattccct gcctgaggtt
     2881 tcagagacca tggccgccta ctgcgatcgc ctggaaacgg gacaggagtt tcaagtagac
     2941 gaatccggcg ccatgcccgc gggtttctgc acctgggcct ggcatgacat tttccccgca
     3001 gtcaccggcc ttcgtttcgg ggggaactat ccctggatga agaaagaagg aatggtcatc
     3061 gcctgctgca gtgacggagc ccggccggtg tttttcagaa tcgaacgagt cgggtaggac
     3121 ccggtgggat ggtaggggcg ggcttgaaac ccgctcctat ggagttcgga atgaatggtt
     3181 cctcagcact cgccgtttgc tgcccgtttc gggcaacatt aacccgattt ccgtaaacca
     3241 gaaaccagca acgagcaagc agaaaccagg aaccaaggac aaattattcc gatttccgag
     3301 ctttcgggga aggggattcc tttctcaaga gcatataaat agccagaagg gctgcgggga
     3361 ttccgatggc ctgagcggtg gaaaggacat tgggaacgac ccacccccgg ggatcatccc
     3421 gatagaattc cactatgaac cgcatcaccg aatagaaaag aagataaaac cagaagagct
     3481 tcccccggaa ccgttctttt ttccgcatgg ccatcagcgc caggaagatt gcgaaattgg
     3541 ccgcggattc atataactgg gtgggatgga gagcgattcc ccggggagcc agagattcgg
     3601 ggtcggtgaa ggtgacggcc cacgggacct ccgtgggtag cccgtagcat cagccggcgg
     3661 agaaacaccc cagccttccg atcgcttggc ccagggggat ggcgggagcg acaatgtccg
     3721 ccagctggcg aaaattcaaa tgatgtttcc gcacgtacca aaggccaacc aggaaggcaa
     3781 agattaaccc gccgtaaaaa accaaacctc cccgccagaa acggacaata tccaccgggt
     3841 tgtcccggaa ctcctcccag ctggcgagaa cataaaagac ccgggagccg attaacgccg
     3901 ccagaagggc gtagaaggca aggtccatca tgatgttcgg gtccagcccc acgcgcttgg
     3961 cctcccgcaa cgctaccagc accgcaagaa agaaagccac cgccagaagc aaaccgtagg
     4021 tgtggagggt gaatccaccg atctttaaga gaattggata cataaaataa agttcggaat
     4081 cggcgccaag atcatcatag gagcgggttt caaacccgac cctacggggt tcgacgttta
     4141 atctttttcc ccaaaggtca gcgatccctt gcgcagcatc tggatgaaca gcagggccac
     4201 accgatggaa atcgccgagt cagccacgtt aaatgccggc cagtgagtat cataccaatg
     4261 gagatcgaca aagtctatca cctcgcccag gcgcaaccgg tcgatgagat tcccgatggc
     4321 gccccccagg atcagggcca ggctcacgag aagggtcttt tggtgctggc gaaggctccg
     4381 gatcaggtaa acgatgcatc cgatggccac cgaggaaata agcgcgaaca aagcgatccg
     4441 cagactcgag cgagtgcccg ctaggaaacc gaaagccgcc ccggtgttgc gcaagtaagt
     4501 gagatgggca aaattgggga tgatctcgac cgactggtgc aaggtcatgg ttttctccac
     4561 caggaccttg gtgcactggt ccagaaccaa gaccccgccg ctgataaggg tcatcaaagt
     4621 gtattttgca ctcacgactt tccttctgcc tgaatggcgg ctagagcggc ctcacagcgg
     4681 ggacagatcg gagagtaacc attcccctct cccacctttg ggtcgtaaac ccaacagcgc
     4741 gggcattttt ggccgccggc ccgagaaacc agaatattca aaccttccat ttccttgctc
     4801 ggctgggccc ccggggctgc ggtgggggac aagaggacct ccgaaacaat aaataattcc
     4861 ttgaggatgg cttcgttttc ccggaggaag ggaaggatct tccccgttcc gctcaaggta
     4921 acggaggctt ccagggagtt tccgatcaac ttgtcctggc gggcagcttc caggactcgg
     4981 gagacttcgg accgcacctt caggagcagc tcgaaccgtt cttccaactt tggatcgaaa
     5041 taccgcgatt ccacctccgg aaagagagtc aggtgcacgc tcttagctcg ttccttcgcc
     5101 ccggggattt gctgccagac ttcctcggcg gtgaaggaga ggataaccgc catgaggcgg
     5161 atcaacgcgt ccaggacctt gtagagggcg gtctgggcgg cccggcgctg gggagaattc
     5221 gccggtgagg tataaaggcg gtccttcagg atatccaggt acaaggcgct caagtccacg
     5281 gagcagaagt tatggattcc gtggaagaca acgtgaaaat cgaaattctc ataagcttcc
     5341 cgcagccggg agttcaattt ctgcaggcga aggaggaccc agcggtccag gtcctggagg
     5401 ctggaatccg ggacggagtc cttcttcggg tcaaaatcgt gcaggtttcc caggaggaac
     5461 cggcaggtgt ttcgaattct ccggtaggca tcggagaggc gggagaggat ttcctcggag
     5521 atgcggatgt cgtcccggta gtcctcggcc gccacccaga ggcggaggac ctcggcgccg
     5581 taccgcttga tgacctcgtc cgggacgata acgttcccca gggactttga catctttctc
     5641 ccttccccgt ccaccacgaa tccatgggtg aggaccgagt tgtagggggc ctggccgcgg
     5701 gttcctaccg aggccagaag ggagctgtgg aaccatcccc ggtgctgatc gcttccttcc
     5761 aggtacatgt ccgcggggga tttcaggttg ggcctttttt cgcagaccgc agcatagctt
     5821 actccggagt cgaaccacac gtcgaggata tccgtttctt ttttaaaatc ctcccccctg
     5881 cacttgggac agcgggttcc cggaggaagg agttccttca ccggaaggtc gaaccaggcg
     5941 tcggctcccc ctttctcgaa gatcttggag acgtattcga ccaggggctc ttcagtcaat
     6001 acctccccgc aggcggtgca ggtgaaagcc acgatgggga cgccccaggc ccgctgacgg
     6061 gagatgcacc agtcggggcg gttctggatc atgccataaa tccggtcatg gccccagtgg
     6121 gggatccatt tcactttgtc gatggcctcc agagcctttt ggcgaaatcc attcttctcc
     6181 atggagataa accattgctc cgtggcccgg aagatgatgg gattcttgga ccgccagcag
     6241 ttggggtact ggtgggtgta gacctcgctg tagatcaggg cacccacttc cttgagcttc
     6301 tgaatgaccg cgtcgttggc gtcaaagaca tactgccccg caaaaaactt cacctctggg
     6361 gtgaaccgcc cttggtcgtc gacgggagaa taaatgggaa ggttgtactc cagcccagat
     6421 tcgtagtctt cctggccgtg accgggagcg gtgtgaacgc acccggtgcc cgtgtccagg
     6481 gtgatgtagg tcgctaggat gagcaaggat tcccggtcca gaaagggatg gcggcacttc
     6541 aagccctcca cttccttgcc cgagaatttt tccaggactt tgtacttctt tttctcggcc
     6601 tgggccatgg cctgctccaa gagcgcttcg gccagaatcc atacctctcc ttccacttcc
     6661 gcggccacgt acttatagtc gggatggaaa gcaatggcca ggttggccgg tatcgtccag
     6721 ggggtcgtgg tccaaatcag gacaaagact ttcttattct tgagcgccgg gaagcggtcc
     6781 ccgaagtcgg agatggccgg aaatttgacc gtgatggaag gggacttgtg gtcttcgtat
     6841 tccacctcag cctcggccag ggcggtttgg cagacggcgc accagtagac cggtttcttg
     6901 ccccggtaca cggaaccttt gcccacaaac cggccaaatt cccgggtgat ggtggcctcg
     6961 tagtcatagc tcatggtgag gtaggggttc tcccactcgc ccatgacccc cagcctcttg
     7021 aactcctgcc gctggatgtc gatgaaccgg gtggcaaact cgcggcagag ttttcttttc
     7081 tccgaaatgg gataggatcc ctttttgctt cccaggctct tgtccacttc gtgctcgatg
     7141 ggcagtccat ggcaatccca gccgggaaca tactccccgt tgaatccggc catgttcctg
     7201 gacttgacga tgaggtcctt gagaatcttg tttagtgcgg ttccgatgtg aatatgcccg
     7261 ttggcgtaag ggggaccgtc gtgaagaata tacttggggc gatctttggc aatctgccgc
     7321 agcttctggt aaattttccc ttcttcccat tttttcagaa tctccggctc gagcttggga
     7381 aggttggctt tcatgggaaa ggcggtcttg ggtaaattca atgtatcctt atagtccatc
     7441 ttttcctcga agcaagggat agctccagct ttttgctggg ctcaaagatg cgcaactcct
     7501 cataatcaaa gtagatcata aatttttatc ataggaggag ccgcggcaca agagaaaaga
     7561 gcgagaaaga ggcaacgctt ttcctattgc ggatgttttt cggcgaatgt tatactgctc
     7621 atgattcaac tcgggccagt cggagattta tcaattcggg taatcatttg gggccgttag
     7681 cctcgggcgt acctagcgga aaggcttggg gctagggata ttttgaaaat gggccttcca
     7741 aaacccaggt ggaaatttcg ccttttccag gtagaaccca cgatgaactg cgtcactcat
     7801 tccagattta tctgtatacc aagaaaggag gtgcttgtct ggggggccgc aaggatgccg
     7861 gatctgtgat tacctttaaa aagagggcgg ccatcggtcc gacccaacat aaagcttctc
     7921 agtcgtttca aaagcacgac cagagggagg gtacgattcg agttatcccg aagtgaaagt
     7981 tagaaattgg aggtgaacaa ctcgggcttt caatcctaaa atcatggagg tataacaatg
     8041 gcgcagcagg gtttatttaa gaccgaagac tggtgggcgg tatggcttgg gatgctggtc
     8101 tttattctta gccttttcta tctcgcagga gcagacctcc tcggctgggt cggtcggccc
     8161 atgatgtgga ccgatttttc gaaatctttc ggccccatct caaaaagtta ttctggcctc
     8221 ggaggatttg gatcgctctt ggtcacctat attgcctttc tggtagctct gaccattggt
     8281 gccaaagcca tcggtttcaa aatcggcaaa tttatctatg gatttacaat cattttctgg
     8341 ctgacgatcc tctgtgtggt cctcggcaat tatgccaata tcgccgccaa ttcaccaggg
     8401 gacattaaga gattcggcct gaattggtcc ttaaggctga ccggagaatc cggcctcatc
     8461 atcgcccttc tggtcggcct attcatcagc aatttcctgc ccagatttgc tgagacgctc
     8521 aaggaagcgg cgcggccgga gtggtatata aaaaccgcca ttgtcattct gggtgcgggg
     8581 atcggagtga aagcggttgg agcgatgggg ctagccacgg cggtgatgtt ccgtggcctc
     8641 tgtgccatca tcgaagccta cctgatttac tgggcgttgg tctattggat ggcccgaaag
     8701 tggttcggct ttagcaggga atggtccgcg cccctggcct cgggcatatc catttgcggg
     8761 gtctcggcct ccattgccac gggcggggcc atccgggcca ggccgatcat acccatcatg
     8821 gtctcctctc tggtcgtcat ctttgccgtg gttgaactat tgcttctgcc ctttctggct
     8881 caaaccttcc tttaccagga acccctggtg gcgggtgcct ggatgggcct ggccgtaaag
     8941 acggatgggg cagcggtcgc cagtggcgcc atcgtagacg ctcttatcaa ggccaaggcg
     9001 cttacggctc agggcatcaa ctatgaaccc ggatggatgc tttcgacggc aaccacggtt
     9061 aaaatcttca tcgacatctt tatcggggta tgggccttta tcctggcggt gatctggacc
     9121 accgtcattg aagctcaacc aggcgtaaaa gtcagggcga tagagatctg gaaccgcttc
     9181 cccaaattca tcatcgggta cgccctgact tttgtgatca tgctggtaat ctgcttcaac
     9241 aatcccggga tcatgaaaaa cgcagtacca gccagtggag aagcggatgt cttccggact
     9301 accttctttg ccctcacctt cttctccatc gggctggtct ctaatttccg gaagctctgg
     9361 gaggagggaa ttgggcgcct ggcggtggtt tacctcgttg ccctctttgg atttatcatc
     9421 tggatcgggc tcttgatttc gtggatcttc ttccacgggg tgaaaccccc cattattggt
     9481 tagcgcataa tcgcaaggag gatctatcat ggcggaaaaa aaggaactgg ttgaagaatt
     9541 gaagaagatg aaagtggagc cattcctgcc cgtagagaaa aagcttgttc tctggagtat
     9601 tatcctggga gtggtcctgc ttggtttcct tgtctgggta agctatacct tcttccccgc
     9661 tggtcattaa tcatagaggt gggacccagg cttttggttg ggtcccacct tgcttctgaa
     9721 gccttctcgc catagttcct gccaggcatg gtcactcttt tcgcctatgc ggcttttttt
     9781 taatgccctt ttaatggaca taggaggcca ttgccatcct ttgttatcca atgttgcctt
     9841 tttgggggcg gggtgacgtt ttcagtgaac ccaaacccct aacagaatca tagaccatac
     9901 ccaaggtttt cattttttct tgacaattcg aaaatgttta aatattcttt tccggggtaa
     9961 tgccctgttc ccaaagaata ggtcccccag gaggcggaat catcctttca taaattttct
    10021 ctattcgatt aaaaatgacc gaaccctatt tctccagcga cttttagaag attctccagg
    10081 gccagggatt acaggttgat gaaatttact taggaggagg tttatggacg tcaaagaatg
    10141 ccactgttta cccgttgacc acctaaccag cgagctagaa acccacctgg aaagagggct
    10201 aacccaaaaa gaagcccaag cccggttgca aaagcatggg cccaatgagc tgacggaaaa
    10261 gccacgggag ggcttcctga agatgctcct ggaccagttt aacaacttcc tggtgatcat
    10321 cctgattatc gccgcagtcg tctccttgct gctgggcgag atcattgacg ccacggccat
    10381 catggtcatc gttatcctga acgcaatcgt gggtgtcatt caagaatcca aagctgagca
    10441 agccttggcc gctctgaaga aaatgtccgc tcccaatgcc caagtaatac gggatggcca
    10501 tcagatgacg atcccctccc gggaactggt gcctggagac cttgtactgg tagaggcagg
    10561 gaattatatt cccgccgaca tgcgtctggt ggagagcgta aaccttaagg tggaagaggc
    10621 ctcgctgaca ggggaatccg ttccagtgga gaaggtggcc ggagtggtgc tggacaaaga
    10681 aatcccgatc ggcgaccgaa agaactccgc cttcatgagc accatggcaa cctacgggcg
    10741 aggcaaaggg ctggtcactg gcaccgggat gcacacccag atcggcatga ttgccgagat
    10801 gctccagtcc tatgaggatg aggcaacccc actgcaacag aagctcgatc agctggccaa
    10861 aacgctgggc atcatctgcc tggcagtttg cgggattatt tttgtctatg gcctcatccg
    10921 ggatacgcat tttggaacga ttatgaatga agggttcata gcttacctcc gggcggagaa
    10981 gaaagatatc attgaacttt tcatgaccgc cgtaagcctg gccatcgctg cggttccgga
    11041 gggtctgccc gcagtggtca cgatctgcct ggccctcggg atgcagtcca tgatccgccg
    11101 gcatgctctg attcgcaaac tcccggcggt ggaaacgctg gggtgcgcca ccgttatatg
    11161 ttcggacaag actggaacgc ttacccagaa cgaaatgacc gttgttcagg catgggcggg
    11221 aggcaagagt ttcaaaatat cgggtgaggg atacaacccc gttgggcagt tctccctgga
    11281 agggaagtct ttcgatgcgg ccaaagaccc gaacgcgtct cttcttctcc atgcggccat
    11341 gctctgtaac gacgccaaac tggaagaggg gagtgaagga aaaggggaga aggcgtggcg
    11401 tatggtgggt gacccaaccg aaggcgctat ggtcgtagca gcagccaaag ctggcctttg
    11461 gcgaaaggag atggaaaaag cccttccccg gatgcaggag atccccttcg actcggaaag
    11521 aaagaggatg accacgatcc accagcagca gaccgacagc gtcaatttct attttgattg
    11581 tccgtccaat gtggccttcg tgaaaggggc accggatatt gtcctagacc tctgcgatca
    11641 tatcatcatg gatggaaaga ccattcccct ttcggaagaa atgaagagaa atgttttggc
    11701 ggcgaaccag gatatggccc ggcaggccct ccgcgtgtta ggagctgcct accgtcctat
    11761 ggatggaatt cccaaccaat gcaggtggga agacatcgag aaaggcctta ccttcatcgg
    11821 acttatgggc atgattgacc cagcgcggcc tgaagcgaaa gaagccgtaa agttggcccg
    11881 gggcgcgggc ttgaagagcg tcatggtaac cggggattac aaggatacgg cggaggctgt
    11941 cgccaaagaa attggccttt taaccccggg cgggcgggtc cttagcgggg cagaactgga
    12001 taaaatgagc gacgaggcac tgaccggcat gatcgataaa gtggatgcct tttgccgggt
    12061 ttcaccgcag cacaagacca gaatcgtcga tgcccttaaa gccaagggcc acgttgtagc
    12121 gatgaccggc gacggggtaa acgatgcccc agctctgaaa agggccaaca tcggcgtggc
    12181 catggggatt accggaactg acgtgtccaa agaaacggcc gacatggtcc tcaccgacga
    12241 taattttgcc agcatcgttt cggccattga agaagggcga atcatttact cgaacatccg
    12301 caagtttgtg tattacctga tctcctgcaa catcggagaa atcctgatca tctttttggc
    12361 catgctggcc ggtgcgcccc tgcccctgag gccggtacaa ttgttgatgt tgaacctggt
    12421 caccgatggc gccccggccc tggctctggg catggagaag ggcgaaccgg acatcatgaa
    12481 gcgtccgccc aggccaacca aggagccggt cataaattgg gaaatgcgga ttggcacgct
    12541 ggtgcaggct gtcgtcatga cggctgccgt actctccgct tatttctggg ccctgcgggt
    12601 ctacccccaa cagctggagc gggcccaaac gattgccttt gtttccctgt gcctgtctga
    12661 gctgctgcga gcctttaccg cccggtcgga gcgttacgga atttttagca tcggcgtctt
    12721 ttcaaacaag tggatgctct gggcagatgg aagttcgctt ctcctggttc tgcttgtggt
    12781 ttatgtcccc ttcctgcggc ccttctttga cac
//
LOCUS       DASPRR010000610         6564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_150397, whole genome shotgun sequence.
ACCESSION   DASPRR010000610 DASPRR010000000
VERSION     DASPRR010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150397"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VEL68_22490"
     CDS             complement(<1..1516)
                     /locus_tag="VEL68_22490"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXZ38814.1"
                     /translation="MAPPENREPIIRRAQKNRPAVLFYGIAFSALLFLLNILNPGLTD
                     FLNNQIYDLLVKMERTGDPSPIPVIIDVDEKSLRQYGQWPWPRHRIATLINKLRELGS
                     LSIGLDMLFAEDDRTSIHVIKKELRRDFGFDLQFRGTPPELMNNDQSLADALSRSAAV
                     LGYQFLFDEDSRPDGCLLHPLRVHRIGNPGKEEEEKLFLRARGAACNLQSLSRAAGGS
                     GFFNIAPDPDGIMRRVPLIIEYKGKLYPSLALATLMRAMAINEAVLKTGRSGEDSLIL
                     DKTAIPLTSQGTMLIRFRGKGKTFGYISAADILSDQVPKNKVQGKILLVGTSASGMKE
                     LKPIPFDPIFPGVEVHATIVDNMLKKDFLLRPKWASGMEAILTLGFGLLSAFILTWTG
                     AGLSSLLLGALAAGASLASLLAFFGGGIFISPISCLLVLGVNFSFLSFLKFWDEEQRA
                     KERALELVMVQEATIESMSSLVETRDPETGGHIKRTQNYIRLLAESLKKRSRFRA"
     gene            complement(2444..2917)
                     /locus_tag="VEL68_22495"
     CDS             complement(2444..2917)
                     /locus_tag="VEL68_22495"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXZ38815.1"
                     /translation="MDHHDVRKLQLIIIASFFALTIHGKAFSADPSPVIGTLQRVSGA
                     AAVVRQDQILPAKAGLEIHSNDTLRTGPDGSIGVVFRDDTQLSLGPGSVLAIDEFVYA
                     PRQGKLSLIFRMMKGTAVYLSGLIAKLAPDSVLFVTPSASVGVRGTKFAVKVEER"
     gene            complement(3381..4832)
                     /locus_tag="VEL68_22500"
     CDS             complement(3381..4832)
                     /locus_tag="VEL68_22500"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HXZ38816.1"
                     /translation="MERDKFLGPNWLLGTREAIRLFRDVAVPFRSRVGILDTHTHHNL
                     RQIVENQPFPNIWRAEVLEDRQEYANNDHYIIQLAAKLPGFSQAFARNPRISDFEKWV
                     ALSRVFPDLEGNHIHQWLHLNLRRLFGINLLLNEKTGERIWNLTSKHFQKKEMLPQAV
                     LKRARARVICTTDDPIDDLRYHLATEKVGGVRFLPTFRPDAYANIFSDSWRSNVDRLL
                     QKTGKKASLQGLVDALGERHEYFARRGARASDHGLLEPYGLEVSQRRAEEVFRAAYGR
                     RKKFGPRSPETKEFISFLMHRFCEMNQKKGMVTQVHYGAFRNANRYLFEKWGADVGGD
                     IVFDQVHIIENITPLLSKFFDGKDSHQSHLVLYPMNQAFTHVNIMLERAFPNAHAGFP
                     WWHNDTPYVMEHYLLHTAGASLLTSSGGPVCDGRKVLSEESRFEVFDRVICRAVGKLM
                     SDGQISKPGGIRLVKALMYENQARLFGLGGGDS"
     gene            complement(4819..6147)
                     /locus_tag="VEL68_22505"
     CDS             complement(4819..6147)
                     /locus_tag="VEL68_22505"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HXZ38817.1"
                     /translation="MIMDFSSLKANKRRLRKDLESLGKFGEGPDGGILRTALSDADLQ
                     AREWFKGRMRDAGLWVREDAVANIIGRLEPASGAQAAPCIATGSHMDAVLHGGKFDGA
                     VGLCCSLEALRAIQESGLSIPCPLELIVFTDEEGAHYAGTFGSRAMLNLLVEDEIYKS
                     RGSGCPFLAQSLERMGKDPRQSGKAARSPSEFRAFLELHIEQGPVLDSLGIPVGVVEG
                     IVAIDRHMIEVEGKAGHAGTTPMRLRDDALVKAAGFITAVNREVRSLGSDIVGTIGEM
                     KVHPGAFNIIPGRVEMTLDLRSMKKSLLRSARGKIHEVLRSIPGARMETVLSKDGSRM
                     DRKIMELIEEVCRQRGVSWKRMGSGAGHDAMTFPTRGIPTGMIFIPCEDGKSHCPEES
                     IRWEDAAIGAQVLADTIVRIAHGSDLNGMSKRAKREKGEMKKDGKRDGER"
BASE COUNT         1480 a         1847 c         1702 g         1535 t
ORIGIN      
        1 aagcccgaaa ccgggagcgc ttcttcaggg actcggccag aagccggata tagttctggg
       61 tacgtttgat gtgccccccg gtttcggggt ctcgcgtttc gaccagggaa gacatgcttt
      121 caatcgtggc ctcctggacc atgaccagct caagggcccg ctctttcgct ctttgttctt
      181 catcccagaa tttcagaaaa ctcagaaagg agaagttcac ccccaagaca agaaggcaag
      241 agattgggga aataaagatg cccccgccga aaaatgctag aagggaggcc agggaagccc
      301 ccgcggcaag ggccccaagg agcaaggaac tcagccccgc tccggtccag gttagaataa
      361 aagcagacag caggccaaat cccaaggtga gaatggcttc catccccgaa gcccatttcg
      421 gtcgtaaaag gaagtccttc ttgagcatat tgtccacaat cgtggcatgg acctccactc
      481 ccgggaaaat cggatcgaag gggatgggtt tcaactcctt catccccgag gcggaggtac
      541 ctaccaataa tattttccct tgaactttat ttttggggac ctgatcagag aggatatccg
      601 cagcagagat atatccaaaa gttttgcctt tgccgcgaaa ccggatcaac atggttcctt
      661 gggaggtgag gggaattgca gttttgtcca gaattaaaga atcctcaccg ctcctaccgg
      721 ttttgagaac ggcctcattt atggccatcg cccgcatcag cgtcgccagg gccagacttg
      781 ggtagagctt gcccttgtac tcgatgatta agggcactcg cctcatgatg ccatctgggt
      841 ccggggcaat attgaaaaaa ccggaccctc ctgcggcccg ggaaagcgat tgtagattgc
      901 acgccgcacc ccgtgctcga aggaagagct tctcctcctc ttctttccca gggttcccaa
      961 ttcgatgaac tcgcagggga tggagcaggc atccgtccgg tcgggaatct tcatcaaaca
     1021 ggaattgata gcccagcaca gccgcacttc tggaaagcgc atccgcgaga ctttggtcat
     1081 tgttcatgag ttccggggga gttcctctga attggaggtc gaatccgaaa tcgcgtcgca
     1141 gctctttctt gatcacgtgg atcgaggtcc ggtcgtcttc ggcaaaaagc atgtccaagc
     1201 ctatgctcaa agaccccaac tccctgagct tgttaatcaa ggtcgcgatt cgatggcgcg
     1261 gccagggcca ttgcccgtat tgcctgaggc tcttttcatc gacatcgatg atcaccggaa
     1321 tcggggatgg gtctcctgtg cgctccatct ttaccagcag atcatagatt tgattattga
     1381 ggaaatccgt aaggccgggg ttaagaatgt tcaggaggaa aaggagggcc gaaaaggcga
     1441 ttccataaaa aagaaccgct gggcgatttt tctgggcgcg gcgaatgatc ggttccctgt
     1501 tttccggggg cgccatatgc tggtaagaaa atggaaagag cctttccgaa caaactttcc
     1561 cgccgggcct ttcctggggc ctcattggaa gcggggaaaa gacgcccacc gggcctttct
     1621 catctcatgt tgaatcccca actccgtacc gacggaaaaa tcagcttccc ccaactatct
     1681 gatcatcact tcaacccttc ggttaagagg ctctgcaacc tcgtcctcgg tatatacaag
     1741 gggccttgac tcgccccaga aggaaacgaa taacacgctc gattggatgc ctttggcaat
     1801 caagaaatct ctgataaagt tcgcccgcct tgaggagagc tttaggttat gctgctccgt
     1861 ccccaaacga tcggtatgac ctgcgatatg gatttccgtt ggtttacgaa tttgaatcgc
     1921 ccttaggatc tccggcagta tcgccttgga tccatccgca agtttggttt tatcgctttt
     1981 aaaccagagg gtgaaagaaa gaaaacgggc ggttgaatct ggctccgccg aaagggcgtc
     2041 cccgaagatg ctggcaatcc ctttttcatc caggggctgg ggcgcaatgg gccggctatt
     2101 aaaatcttca acccgggtcg tgtcccctgg cttgtcaagg atttgatagc ctcctgcgtt
     2161 tgcaatgcgg atggaaccgg ccttgccatc cggttcaggc aagaggacga acaggtcgcc
     2221 cttggaggtc tctgtttcac cttggggctg ttcaacttcg gcatagggga ccgatggtga
     2281 aaccattaga tgggggttgc tggcgcatcc gacaaggagg aacaataagg gccatagagt
     2341 ctgaactctg ggcgataatt tcattgggtt aactcaggta tccttccgga aaagcctttt
     2401 gccgtccaga gcgccgccgc tcggagaaag gaccgcttga tagctacctt tcttcgactt
     2461 tcacggcaaa ttttgtgcct cgtactccta cggaagcgga gggagtcacg aaaaggaccg
     2521 aatcaggtgc aagcttggcg atgagcccag acaggtaaac ggcagttccc ttcatcattc
     2581 gaaaaataag cgatagcttg ccctgccgcg gagcatacac aaattcatca atggccaaca
     2641 cgctgccggg tcccaaagac aattgggtat cgtcgcgaaa cacgacgccg atggacccat
     2701 cgggacccgt acgaagggta tcattggagt gaatttccaa gcccgctttg gcgggtagga
     2761 tttgatcctg tctcacaacc gccgctgctc cgcttaccct ttgaagagtc ccgataaccg
     2821 gggaaggatc agcggaaaac gccttcccat ggatggtcag cgcaaaaaag gaagcaatga
     2881 taataagttg cagttttctt acgtcatgat gatccaaaat cgaacccccc ggctcttttc
     2941 tctgttcccg tgattgcatt accaaaccag caagaatcaa gccaaaagga tcgcagctgg
     3001 gatataaata atatcaaatg gttaaaacca tttttcagct taatgccttt agcgggggta
     3061 caaaattagt caggattttg ccagggtgag cgcaaaattg tcaaacaatg aggttatggg
     3121 aatgggaggc acgattccct ttattttctc gcgttcccgt tgaacttctg catctatcgg
     3181 atagtccgct ttcttcctcc tgggtcttgg agactggata gatcgttgtc gatggggtaa
     3241 atgaaatcgt gggaagaaaa agaaacggag ttggcgagtg catggttcgg gagggaggag
     3301 ggagagcaaa tcttgaccac aggggtggtt cgcaaaccgc ctctacacca ggaaatctat
     3361 gaaaatattc cagaggcaat tcatgaatcg cccccgccca atccgaataa tcttgcctga
     3421 ttctcataca tcagcgcttt taccagccgt atccctccgg gttttgaaat ctgcccgtca
     3481 gacattagct tgcccaccgc ccggcagatc acccggtcga aaacctcgaa gcggctctct
     3541 tctgacagga cctttctccc gtcacagacc gggccgccgc tggaggtaag cagggacgct
     3601 cccgcggtgt gcaggaggta gtgctccatc acgtaagggg tgtcgttatg ccaccagggg
     3661 aatcccgcat gggcgttggg aaaagcccgc tccagcatga tgttcacatg ggtgaaggcc
     3721 tgattcatgg gatacaggac gaggtgggac tggtgagaat ctttcccgtc gaagaacttg
     3781 gaaagtaaag gggtgatatt ttctatgata tgaacctgat cgaaaacaat atccccccct
     3841 acatccgccc cccacttctc aaacaaatac cggttggcat tccggaaagc cccatagtgg
     3901 acctgggtga ccatcccctt cttctggttc atctcgcaga accggtgcat caggaaagag
     3961 atgaactctt ttgtttccgg agaccggggc ccgaacttct tccttctccc gtaagccgcg
     4021 cggaagacct cctccgccct tctctggctt acttccaacc cgtagggttc caggaggcca
     4081 tggtcgctgg cccgcgctcc cctccgggca aagtattcgt ggcgctctcc aagggcatcc
     4141 accaagccct gaaggctcgc tttctttccg gtcttctgca acagccggtc cacattggac
     4201 cgccaggaat cgctgaagat gttggcgtag gcgtccggcc ggaaggtggg aagaaaccgg
     4261 acccctccga ccttttctgt cgcgaggtga tatcttaggt catcgatcgg gtcatcggtg
     4321 gtgcagatca ccctggctcg ggcccgcttt aggacagcct ggggaagcat ctcctttttc
     4381 tggaaatgct ttgaggtgag gttccagatt ctttctcccg tcttctcatt cagaagcagg
     4441 ttaatcccga agagacggcg aagattgaga tggagccatt ggtgaatgtg gtttccttcc
     4501 aggtccggga agacccgact gagggccacc catttttcga aatcagaaat ccgggggttc
     4561 cgggcaaaag cctgggaaaa acccgggagc ttggccgcta gttggatgat atagtgatcg
     4621 ttgttggcat actcctgccg gtcttccaga acctccgccc tccatatgtt gggaaagggc
     4681 tggttctcca cgatctgccg caggttatga tgggtatggg tatccagaat tccaacccgt
     4741 gaccggaagg gaacggctac atcccggaac aaacggatcg cttcccgggt acccaggagc
     4801 cagttgggac cgaggaattt atctctctcc atctctcttc ccgtccttct tcatttcccc
     4861 tttttccctt ttcgcccttt tgctcattcc attgagatcg ctaccatggg caattctcac
     4921 gattgtatcc gccaggacct gggccccaat ggccgcatcc tcccaacgga tggactcttc
     4981 cgggcaatgg cttttgccat cctcgcaggg gatgaaaatc atgcccgtgg gaatgccccg
     5041 ggtgggaaag gtcatggcat cgtgaccggc gccactgccc attcgtttcc aggaaacgcc
     5101 cctctgccgg cagacctcct cgataagctc catgatcttt cggtccatcc tgctcccgtc
     5161 cttcgacagg acggtctcca tgcgggcgcc cgggatggag cgaagaacct cgtgaatctt
     5221 tcccctggcg cttctcaaga gggatttctt catggagcgg aggtccaggg tcatctccac
     5281 cctgccaggg atgatgttga acgctcccgg atgaaccttc atttccccga tggtccccac
     5341 gatatccgac cccaaagatc gcacttcccg attgaccgcg gtgatgaagc cggccgcctt
     5401 caccaacgca tcgtcccgaa ggcgcatcgg ggtggttccg gcgtgccctg cttttccctc
     5461 aacttcaatc atatggcgat cgatggccac gattccctca actactccta cgggaattcc
     5521 gagcgaatcc agaaccggcc cttgctcgat gtgcagctcc aggaaagccc gaaactccga
     5581 gggagaacgg gctgccttgc cggactgccg cgggtctttc cccattcgtt ccaagctctg
     5641 ggccaggaag gggcatcccg atccccggga tttataaatt tcgtcctcca ccagaagatt
     5701 caacatggcc cggcttccga aagtcccggc gtaatgcgcc ccttcttcat cggtgaagac
     5761 aatgagttcc agggggcaag ggatcgatag accgctttcc tggatggccc gcagggcctc
     5821 caagctgcag cacaggccta cggcgccgtc gaattttccc ccgtgcaaaa cggcatccat
     5881 gtgcgatccc gtggcaatgc atggcgccgc ctgcgccccg gaagccggtt cgagtcgccc
     5941 gatgatgttg gccactgcat cttcccgaac ccataaaccc gcatcccgca ttcgcccttt
     6001 aaaccactcc cgggcctgca ggtcggcgtc ggatagggct gtcctcagaa ttcccccatc
     6061 cggcccttcc ccaaattttc ccagactctc gaggtccttt cgtagccttc ttttatttgc
     6121 tttgagtgat gaaaaatcca ttatcatttt caccttgccc cctgtttccg caccacaaat
     6181 cttcggtctt cctctcctct acccagctct cgcccatccc aattcattcc gactccccat
     6241 tggccaccgc tcccgatgag aaacgggcct ttggagaccc tcttactcct gacggctcca
     6301 tggattgccc cagcaggtct attttcatgg ggattcaggc gaggttgatt ttcttgaagc
     6361 ttttgctatc gatcccttcc ggcagaggac tttcattttt ataatccctt gccggggttt
     6421 attgaatgta acttcccggg gcaataaatt caagggataa atctttcctg atcattcttt
     6481 tgcccgggcg agataaggac cacgcccagc ttgagaaaca atctggtttc gtatagaata
     6541 gaattatcca tgggttccca aaaa
//
LOCUS       DASPRR010000611         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1117012, whole genome shotgun sequence.
ACCESSION   DASPRR010000611 DASPRR010000000
VERSION     DASPRR010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117012"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..1334)
                     /locus_tag="VEL68_22510"
     CDS             complement(378..1334)
                     /locus_tag="VEL68_22510"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXZ38818.1"
                     /translation="MAKIFIDELSKGQTVESIFLVRDKILAKTKAGNPYLSIKLADRT
                     GEAEGRIWDSALDFLPLFEKDDFIKVRGEVDEFQGMQQLRITRLRKCEDREIQLGDYL
                     PQTSQDVEKMLGELTNIASQVRQPFLGRLLEAFFKDEELIRKFKLAPAAKAIHHVFLG
                     GLLEHTLSVVQLVLLIGPRYKGIDQELLLTGAILHDLGKVSELSYERTFDYTDPGRLL
                     GHITLTVEMIDEKIKGISDFPENLAILLKHIILSHHGEYEFGSPKRPKTLEALLLHHL
                     DDMDAKMNGFLAWIEKEKDTPSRWTSYHKLFERFIYKPEEEG"
     gene            complement(1443..3038)
                     /locus_tag="VEL68_22515"
     CDS             complement(1443..3038)
                     /locus_tag="VEL68_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXZ38819.1"
                     /translation="MTIRVSPINLTLAEEEASLYQKTAQMLRLSMEELHEIRITKKSI
                     DARRKNRIHFVYAVELSLSLEEEQRLLGQPPPALRVEEVPPKSVPAPTVIKSKPRNRP
                     LIVGTGPAGLFAAWKLTRSGLPPLILERGREVSARVKDVNRFWEEGILEDESNVQFGE
                     GGAGTFSDGKLYTRLNDPGISEILEAFVRFGAPPEIQYLQRPHIGTDRLRRAVTGMRQ
                     FLQEQGAEFRFQAKVSDLKIAKGRLEGVVVNGREEIDGAILFLALGHSARDTYRMLQH
                     TGIAMEPKPFAIGLRIEHPQRLIDRIQYGPSAGHPRLPPAEYQLTYRTPKGRAVYSFC
                     MCPGGAIIGSSSEKATIATNGMSYFRRNSPWANSALVVSVGKEDFGGEDPLAGMDFQQ
                     RWEEKAFQLGGGNFRAPAQAVYDFLEGKEPGPAKKTSFRPGVTPARLDECLPGFVAES
                     LREAIPYFDRKMRGFSSKEAMLMGVETRTSSPIRILRREDFQSQNVHGLFPVGEGSGY
                     SGGIISSALDGMKAAEALLKRLG"
BASE COUNT          761 a          950 c          931 g          844 t
ORIGIN      
        1 aggctgtaag aagcgtctgc ggcagcgatc gttttgaagg gggctttagg cgggcggagg
       61 cgaagccttt ttcgcaactc ctcctgaatc ccgatggctt tcttagggga tacgttccag
      121 ggatggagga ttggcatttt caatttttca ccacagaggc acagaggcca ctgagataga
      181 ggaaaaagat tccaaggtgt ggataacgac caagaagcca ggcgactccc aagcttttcc
      241 tttcatcccc aacactccca gaaatccccc cttcgccccc ctttctaaag gggggtaggg
      301 ggattttaga gagtttttgc ttatgaaccc atattcaatt ccttctctgt gccctccgag
      361 tctcggtggt gaaaagatca gccttcttct tccggtttat agatgaaccg ctcaaagagc
      421 ttgtggtagg aggtccaccg ggaaggggta tccttctctt tttcgatcca ggccaggaat
      481 ccgttcattt tggcgtccat gtcatccagg tgatgaagga ggagggcttc caaagtcttg
      541 ggccgtttgg gcgagccgaa ttcgtattcc ccgtgatggc tgaggatgat gtgcttgagg
      601 aggatggcca gattctccgg gaaatcggaa atgcccttga ttttttcatc gatcatttcc
      661 acggtaaggg taatgtggcc caggagtcgg cccgggtcgg tataatcgaa ggtccgctcg
      721 taggacagct cggatacttt acccaggtca tggagaatgg ccccggtgag gagaagttcc
      781 tgatcgattc ctttgtaccg gggaccaatc aggagaacca gctgaacgac ggaaagggta
      841 tgttccagca ggccgccgag gaaaacgtgg tggatcgcct tggccgcagg ggccaattta
      901 aattttctga tcaactcctc atccttaaaa aaagcttcca ggagtctacc tagaaagggc
      961 tggcgaacct gggaagcgat attcgtgagt tctccgagca ttttctcaac gtcctgagaa
     1021 gtctggggca gataatcccc cagctggatt tcacggtctt cgcatttcct cagcctggta
     1081 atgcgaagct gctgcatccc ctggaattcg tccacttccc cccgaacttt tataaaatcg
     1141 tctttttcaa acaggggaag aaaatccaga gcgctatccc aaattcgtcc ctctgcttcc
     1201 ccggtccggt ctgccagctt aatggagagg tacggatttc cggccttggt tttggccaaa
     1261 atcttatccc taaccaggaa gatgctctcc accgtctggc ctttactcag ctcgtcgatg
     1321 aatatcttgg ccatattccc ccccccagaa aaagggcgaa aaggcgaaag gggaaaaagg
     1381 cgaagaggaa aaatgattat caaagaaaca agaacttttt cgccctttcc ccgtttcccc
     1441 ttttacccta gtcgttttaa taatgcttcc gccgccttca ttccgtccag ggcggagctg
     1501 atgatgccgc cggaataccc ggacccctcc ccgacgggga aaagaccatg gacattctgg
     1561 gactgaaaat cttctctccg gaggatgcgg attggagaag aggttctggt ttccaccccc
     1621 atgagcatgg cttctttgga tgaaaatccc cgcatcttcc ggtcgaaata agggatcgct
     1681 tctcgaaggc tttcggcgac gaatccggga aggcactcat ccaggcgggc tggagtgacg
     1741 ccgggccgga agctggtctt tttggcggga cccggttcct tgccttcgag gaagtcatag
     1801 accgcctgag ccggggcccg gaaattgcct ccccccaatt gaaaggcctt ctcctcccat
     1861 cgctgctgaa agtccatgcc ggccagggga tcttctcccc cgaagtcttc ttttcccacg
     1921 ctgaccacca aggcgctgtt ggcccagggt gagttcctcc ggaaatagct catcccgttg
     1981 gtggcgatcg ttgctttctc agaacttgac ccgatgatgg ctccgcccgg acacatacag
     2041 aaggaataga cggcccgccc cttgggggta cggtaggtta gctggtattc tgcggggggc
     2101 aggcggggat gccctgccga aggcccgtac tgaatccggt cgataagcct ctggggatgt
     2161 tcaatccgga ggccaatggc gaagggcttg ggctccatgg cgatgcctgt atgctgaagc
     2221 atccggtacg tgtcccgggc gctgtgccca agggccagga agaggatggc cccatcgatc
     2281 tcttcccttc cgttgacaac tactccctcc agccttcctt tggcgatttt caggtccgaa
     2341 accttggctt ggaaacgaaa ttcggctccc tgctcctgga gaaattggcg catgccggtt
     2401 accgccctgc ggagacggtc ggtcccaata tgaggccgtt ggagatattg gatttccggg
     2461 ggagctccga aacggacaaa ggcctccagg atttcagaaa tgccgggatc gttaagacgg
     2521 gtgtagagct taccatcgga aaaagttccc gcccccccct cgccgaattg tacgttactt
     2581 tcgtcttcca ggatcccctc ttcccagaaa cgattcacat ccttgacccg ggctgaaact
     2641 tccctccccc tttccaagat caagggggga aggccgctcc gggtcagttt ccaagcggca
     2701 aagagacccg ccggtccggt tcccacgatc agggggcggt tcctgggttt gctcttgatt
     2761 acagtgggag cggggacgga tttcggaggt acctcctcga ctcgaagcgc ggggggaggc
     2821 tgccctaata aacgttgttc ctcctccagc gacaaggaaa gctcgacggc ataaacaaaa
     2881 tgaatccgat tcttccggcg ggcgtcaata gacttcttgg tgatacgaat ttcatggagt
     2941 tcttccatcg acaatcggag catctgagcc gttttttgat aaagactggc ttcttcctcc
     3001 gctagagtga ggttgatggg ggaaacccgg atggtcattt gccgtatact aaggggtcca
     3061 taatggacaa gacagattct tccgaaaatc tactctaacc actattcttt cactcaggct
     3121 gtcaccgttc accctgagct tgtcgaaggg tgggtcgcca tgccgttcat gcttcgacaa
     3181 gctcagcacg aacggcttat tcttcagtgt ctatccaatt atagcctgat tggtaattta
     3241 acatattcat tccggcggtt ccatgggaaa tggactcccc cgaatatccc agccgcccct
     3301 cccggggctt cccccccctt tacgttttcg attctctttt ctgcgaaaaa gcaggcttgt
     3361 cgcttttgct ttcgggccgg gcaatgcctg aaattcatag gccctacccc acttgccgaa
     3421 aaatgaagga gacaagcccc tggaaggttt gaactttggc cgaccatgtg ctatttttta
     3481 taccta
//
LOCUS       DASPRR010000612        11748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_630075, whole genome shotgun sequence.
ACCESSION   DASPRR010000612 DASPRR010000000
VERSION     DASPRR010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11748)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630075"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VEL68_22520"
     CDS             complement(<1..1053)
                     /locus_tag="VEL68_22520"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXZ38820.1"
                     /translation="METYLLVSLILVMVLFLFLEGFLSGSEIAMVAADRKKLSGLARS
                     SSRMDRLTLRVLKDPSWFLSTTLVGSNLAEVTNAALVTSILVSAYGSRGDLYAFLILT
                     PFILILGEILPKALFQQKADSWVRKLVPPVWAASILLYPLVWFMSKATLAVSRFLKQE
                     GEVRPLVTREELQLLLREEGERSDINPLEQAMIRRIFRFPTTKVREAMIPLVEVVALE
                     ETCALEEAIASVRRENYSRYPVYRNRVDNIVGILYSLDLLGAEGEGKNIRPYIRPISF
                     FPETKPVDELLVEMQRSQETLAAVVDEYGGAVGIVTVEDILEEVVGEIEDEYDINQPF
                     YRKMGPRKLWINARMEI"
     gene            complement(1073..2122)
                     /locus_tag="VEL68_22525"
     CDS             complement(1073..2122)
                     /locus_tag="VEL68_22525"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXZ38821.1"
                     /translation="MRLYLIFFLFWGLVFFATSEASLFALGRLRLRRLKEERHPAHPL
                     IEHLLSHPRRLIISLLVGNEAVNVAISSLTSSLFITAWGDGAKWLAIPVVVLGILLLG
                     EVIPKTMGVRYPDKIAPAIASHVSRFVSLVGPVHWIIRKIVDGAFRLARVSPEPPSSY
                     LTEEDFKELIEASHKEGALEEGEKHLIHRVFKFSDQVVRNIMTPRAAVFALPLSTRFK
                     EAGEVLRENRFSRVPVYEKNLERVVGILHAKELLRTWGQKRQDDRGLRPFLRKPHFVP
                     QSKKLDVLFREFQKQRIHLAVVVDEYGKTAGIVTLEDLLEELFGEIYDELDEEGRRGR
                     KPIRRGKGRPALQVP"
     gene            complement(2107..2352)
                     /locus_tag="VEL68_22530"
     CDS             complement(2107..2352)
                     /locus_tag="VEL68_22530"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HXZ38822.1"
                     /translation="MKLREIGEFGLNARIKNSWSAISPQVIKGRGNGLVFSVDLLIED
                     VHFDLSFIDPYPLGRKSLAGNLSDLAAMGATPRCDST"
     gene            complement(2374..4806)
                     /gene="lon"
                     /locus_tag="VEL68_22535"
     CDS             complement(2374..4806)
                     /gene="lon"
                     /locus_tag="VEL68_22535"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXZ38823.1"
                     /translation="MANILEDEQMKVPEMLPMLPVRDTVIFPYMILPLFVGRESSIKA
                     VDEALAKDRMIFLATQKVSTDDNPTPESIYSVGTAAMVMKMLKLPDGRVKILVQGLAK
                     GTIREYIQEKPLFVVRIQRVIEPSVGETSLETEALIRSVKEQSEKVLSLKGILSPDVL
                     AILDQIEEPGRLADLVASNLKLKIEEAQKILETFNPLQRLKKVNEFLSKEVQVSTMQA
                     KIQSQAREEMSKTQREYFLREQLKAIKAELGEIDEKAQEIGELREKIKKAKMPSEVEK
                     EANKQLNRLEQMHPESAEATIVRTYLDWLVELPWAVSTEDNLDIKKAKQVLDEDHYDL
                     EKVKERILEYLGVRKLKEKMKGPILCFVGPPGVGKTSLGKSIARSLGRKFTRISLGGI
                     RDEAEVRGHRRTYIGSLPGRIIQGIKTAGSNNPVFMLDEIDKVGADFRGDPSAALLEA
                     LDPEQNFAFSDHYLNVPFDLSKVMFITTANILDPIPPALKDRMEVINLAGYTSEEKLK
                     IAKQFLLPRQWEENGISKESLSMSDEALLRIISQYTKEAGVRNLEREIASVCRKVARR
                     IAEEEKGPFQVSGINLHKYLGPPKYLPEEELEVNEIGVATGLAWTSVGGEILHVEATT
                     MKGKGNLTLTGHLGDVMKESAQAALSYARSKAKELDLDPEFYEKLDLHVHVPAGAIPK
                     DGPSAGVTMATSLISALTRIPVRKDIAMTGEITLRGRVLPIGGVKEKTLAALRAGIKN
                     VILPYQNQKDLDEIPQYIRRRVDYFFVKDMDEILKKALVPEKAQLKKGGAEREPGEKK
                     GRAESAPLSLPV"
     gene            complement(5120..7036)
                     /locus_tag="VEL68_22540"
     CDS             complement(5120..7036)
                     /locus_tag="VEL68_22540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXZ38824.1"
                     /translation="MKDIFSPSGLLAKNIPFYEYRPEQEQMAGAVQRAMAQERFLIVE
                     AGTGTGKTLAYLIPSVLSGKRVVVSTGTKTLQEQLFFKDVPLVQDKLRFSFRAAFMKG
                     RGNYLCWRRFRLFSRQPLFASMEEVGYYQTVKKWAGKTKTGDRAELTDLPEDLGLWKE
                     VCASSETCLGQSCEFHDPCFIMVMRQEAAGADILIVNHHLFFADLAVRLKGYGEVLPR
                     YEAVVFDEAHQLEETATQYLGSTISNYRFEELARDLRRECTAAKVKEGTLTRIATDLL
                     DLEERFFQPLRGKEARFPLQKERWKEDTKETGGRVMDKLTSLSSHIAGMQEPSEGLRA
                     CGRRAEELKLEFQEFISLAREEMVYWGEVRGRGVFLHGSPVDVSSALQENLYPRLKTA
                     VFTSATLSTQGNFNFFRSRMGLTGGGGEGTDELILDSSFDLKAQSLLYLPPHLPEPNS
                     PSFLPRAVEEMEKILRLSLGRAFLLFTSIRNMEEAYRRLKERVPFTCFLQGERPKTAL
                     LKAFKDDLHSVLFATASFWEGVDIQGEALSCVVIDRLPFSPPTEPILEARLERIAAAG
                     GKPFWDYQVPSAILLLKQGLGRLIRTRQDRGILAILDTRLLTRGYGKVFLQSLPPWVI
                     AHEPEAIDRFFRNS"
     gene            complement(7033..7932)
                     /locus_tag="VEL68_22545"
     CDS             complement(7033..7932)
                     /locus_tag="VEL68_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38825.1"
                     /translation="MINDHRLPTTDTGRMTRGAKGVPGLKIHGWIGLAIIGISEILLF
                     SGVSLVRTFFTPLVWSGYILFVDSLVHLHRGTSWVLNRPKKLLLLLPVSIGFWLIFEF
                     YNLYLRNWHYVGLPEEVSLRLLGYAWAFATIWPAILTTAEAMRGWKRISLSKGRPWKV
                     SPMALLFSLAFGGLCLILPLITPSPIAHYLAAPVWVGFIFLLDPLNYWMGRGSLLLDL
                     QKGYPGRLYSLLLSGAACGFLWEFWNFWAGARWYYTVPILGDIKVFEMPVLGYLGFPP
                     FAVECFVMYSFFNNIFLHLGDFR"
     gene            complement(7967..8743)
                     /locus_tag="VEL68_22550"
     CDS             complement(7967..8743)
                     /locus_tag="VEL68_22550"
                     /inference="COORDINATES: protein motif:HMM:NF020476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HXZ38826.1"
                     /translation="MKNLLVLSASRRTDLVGCFPKVLGERLREYPPESVHSLVIWTKN
                     PRNLVIEGELKETLKRYRQIYLHLTITGMGGGEFEPLIPPWEETVGMIAPLVEMVKDP
                     GRISWRFDPILEVEGREGTYSNLHSFPQLCAAIAPWGINTCRVSWVSPYKKVLRRLTG
                     RGWTLISEDLENRKKQVEGLKKIAKTYGMELFFCSMEGLPRSRCIDGELLSRIHPDGL
                     SCSREKARGQRPLCGCTQSLDIGWYSLKCRHGCLYCYATP"
     gene            complement(8955..10352)
                     /locus_tag="VEL68_22555"
     CDS             complement(8955..10352)
                     /locus_tag="VEL68_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ38827.1"
                     /translation="MRKILAVDDELSVRDSLRMIFKKDYQVVTAGSAREAWEKILSEE
                     PDLIFLDIIMPQKDGMELLKEIRESQPLTPVVMLTAAKNLKTAVEAMKLGAYDYITKP
                     FDVEELKIIADKALESRDLKAENRRLQTEIEERYRFDNIIGKSKEMREIYATIAQIAE
                     RNSTVLIHGESGTGKELVARAIHYNSPRKNKPFVAVNCAAIPETLIESELFGHEKGAF
                     TDAQNRRIGHFELAHQGTLFLDEVSELSLPTQAKILRSLQEREFVRVGGTKTIQVDVR
                     LISATNKNLEEMMGRGSFRSDLYYRINVVPLTIPPLRQRKEDIPLLIQHFLEKHAGPG
                     KQKISMEAMEILAAYDWPGNVRELENIIERIVVLSCTDIVTPNDLPVSLKSPSRTEHI
                     KQGVLEGRISFEDAERDFERDIILEALKKSRYIQTKAADLLGISRRILKYKMDKYGIS
                     TSSEEAAVSPEGLQA"
     gene            complement(10378..>11748)
                     /locus_tag="VEL68_22560"
     CDS             complement(10378..>11748)
                     /locus_tag="VEL68_22560"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXZ38828.1"
                     /translation="ASSRLPVFIQGEEGTGKGDLARVLHFLGPWKMSPFLHFPCRGLT
                     PESFVEKILLWGKERKMSPKLSLTLFLENLESLEEEMQALLQDLLSSRQIPWPGLQET
                     TFDVQVLSSSSGDMTEPVTAGKFREDLLQTLEILTLRLPPLRERKEDLPRMVQEILRE
                     KAGERGGSKIFSPEALQALKEYDWPGNLAELESLVLRSAFLKEGNLIEPADLAFSPSR
                     GRSLRETPCGDEKDSWFDVTIPTLAHEIKNPLVAISTFAHLLPDKYEDPEFRQEFSRL
                     VNQDVRRINALFENLLEFAQFSTPQSSLQDLNATLEEFLGKQGKFPGQAERKIVADLG
                     NALPPILFDKTQLNFVLRNLFEHVLFKGNDNFPLHITTHFTREEGAEGRPDWVDMILW
                     YNSPEGVLGNLSRVVGFETKPEFQNLNLALLLTRKVMLRNRGKMQVLLEEEGGMTLRI
                     QFPAGM"
BASE COUNT         2509 a         3375 c         3220 g         2644 t
ORIGIN      
        1 aatctccatg cgggcgttga tccatagctt tctgggcccc atttttcggt aaaagggctg
       61 gttgatgtca tactcgtctt caatctcccc gactacctct tccaggatgt cctccacggt
      121 cacgatgccc acggcccctc cgtattcgtc cactactgcg gccagggttt cctggcttcg
      181 ctgcatctcc accagcagtt catcgaccgg tttggtctcc ggaaaaaagg atatggggcg
      241 gatataaggc cggatgtttt tcccctcccc ttccgctccg aggagatcaa gactatacaa
      301 aatccctacg atattgtcca cccggttgcg atagaccgga taccgggaat agttctcccg
      361 gcggaccgaa gcaatggcct cctccaaggc gcaggtttcc tccagggcca ccacctccac
      421 caggggaatc atggcttccc tgacctttgt ggtggggaaa cgaaagatcc ggcggatcat
      481 ggcttgttcc agggggttga tgtcgctccg ctccccttct tccctgagga gcaactgaag
      541 ttcttcccga gtgaccaggg gccgcacttc cccctcctgc ttgaggaaac gggagacggc
      601 cagggtggct ttggacatga accataccaa agggtataaa aggatcgaag ccgcccagac
      661 tggggggacc aactttctca cccaggaatc ggccttctgc tggaaaaggg ctttgggcaa
      721 aatctccccc aggatgagga tgaaaggggt cagaatcagg aaagcgtaca agtcgcccct
      781 ggagccataa gcacttacca ggatggaggt caccagagca gcattggtta cctcagccag
      841 attgctgcct accagggtgg tggaaagaaa ccaggaaggg tccttcaaga cccgcagggt
      901 cagacgatcc atccgggagg aggaacgggc caggccgctc aacttcttcc ggtcggccgc
      961 caccatggca atttccgatc ccgaaaggaa tccctccagg aagagaaaaa gaaccatgac
     1021 caggatcagg cttacgagaa ggtaggtctc catttgattt ttttctctcc gctcagggga
     1081 cttgaagggc tggcctgccc ttaccccgtc ttatgggttt ccttcccctg cgtccttctt
     1141 cgtccagctc gtcgtagatc tctccgaaaa gctcctccag caagtcttcc agagtcacga
     1201 ttccggcggt cttgccgtat tcgtccacca caaccgccag gtggatcctt tgcttctgga
     1261 actcgcggaa aaggacgtcg agcttcttgc tctggggaac gaaatggggt ttgcgcagaa
     1321 aaggcctcag cccgcggtcg tcctggcgtt tttggcccca ggtccgcaga agctccttgg
     1381 cgtggaggat tcccacgacc cgttccaggt tcttctcgta aactggaact cgggagaacc
     1441 gattctcccg gaggacctcc cctgcctcct tgaaccgggt ggagagggga agggcgaaga
     1501 ccgcagcccg gggagtcatg atgttccgga ccacctggtc gctgaattta aataccctgt
     1561 gaatcaggtg cttttcccct tcttccaggg ccccttcctt gtggctggcc tcgatgagtt
     1621 ccttgaaatc ctcttcggtc aaataggagg aaggcggctc cgggctcacc cgcgccagcc
     1681 ggaaggcccc atccacaatc ttgcggatga tccagtggac cggtcccacc aggctgacaa
     1741 atcgggacac atgagaggca atggccggag cgatcttatc cgggtatcgt acccccatgg
     1801 tcttgggaat gacttcgccc agcagcagga ttcccaacac cactacagga atggccagcc
     1861 acttggcccc gtccccccag gcagtgatga aaagagagct agtcagggaa gagatggcca
     1921 cattgaccgc ttcatttcct accaaaaggg aaatgatcaa tcgccggggg tggctcagga
     1981 gatgctcgat cagcggatgg gcgggatggc gttcttcctt gagtcttcgc agtcgaagac
     2041 gccccagagc gaatagagaa gcctccgaag tggcaaagaa gacgagcccc cagaaaagga
     2101 aaaaaatcag gtagagtcgc aacggggcgt cgctcccata gcggcgagat cgctgaggtt
     2161 gcctgccagc gatttgcggc ctaaagggta gggatcgatg aaagaaagat cgaagtgaac
     2221 gtcttcgatg agcaggtcca cggagaagac gagaccgttc cccctgcctt tgatcacctg
     2281 cggggaaatc gcggaccacg agttcttgat ccgggcattc aggccgaatt cgccgatctc
     2341 gcgaagtttc accgagcggt ccgcaagaag aggtcagaca ggaagggaga ggggagcgct
     2401 ctctgctcgc cccttcttct ccccgggttc tctttcggcc ccccccttct tcagctgggc
     2461 cttctccggc accagggcct tcttcaggat ttcatccatg tctttgacga agaagtaatc
     2521 gaccctccgc cggatgtact gaggaatttc gtccaggtct ttctggttct ggtatggaag
     2581 aatgacgttc ttgatcccgg ccctgagggc cgccagggtt ttttccttga cccccccgat
     2641 gggcagaacc cggccgcgga gggtgatctc cccggtcatg gcgatgtctt tccggaccgg
     2701 aattcgggtc agggcggaaa tcaaagatgt ggccatggtc acgcccgccg aaggcccatc
     2761 cttggggatt gccccggctg gcacatggac atgcaggtcg agcttctcgt agaattctgg
     2821 gtccaggtcc aattccttgg ccttggaccg ggcgtagctc agggcggcct gggcggactc
     2881 cttcatcacg tcgcccaggt gtccggtgag agtgaggttc cccttgccct tcatggtggt
     2941 ggcttccaca tgcaaaattt ccccccccac cgaggtccag gccagacccg tggccactcc
     3001 gatctcgttg acctccagct cttcttccgg caggtatttg ggcggcccca ggtatttgtg
     3061 aaggtttatg ccgcttacct ggaacggacc cttctcctct tcggcgatcc tccgggctac
     3121 ctttcggcac accgaagcga tctcccgctc caaattgcgc actccggcct ctttggtgta
     3181 ctgggagatg atcctcagaa gggcctcatc gctcatggag agactctctt tgctgatccc
     3241 attctcctcc cactggcggg gcagaaggaa ctgtttggcg atcttcagct tctcctcgct
     3301 cgtatagccc gccaggttga tgacctccat gcggtccttc agggccggcg gaatggggtc
     3361 caggatgttg gccgtggtga tgaacatgac cttggacagg tcaaagggaa cgttcaggta
     3421 gtggtcgctg aaggcaaaat tctgctctgg gtccagggcc tccaaaaggg ctgcggaagg
     3481 atcgccgcgg aaatcggccc cgaccttgtc gatttcatcc agcatgaaga ccgggttgtt
     3541 cgacccggcg gtcttgatcc cctgaatgat ccggccgggc agggaaccga tgtaggtcct
     3601 ccggtgcccc cgaacctccg cctcgtcccg gattcctccc aaagagatcc gggtgaactt
     3661 ccttcccaga gaccgggcga tggattttcc cagagaggtc tttcccacgc cgggaggacc
     3721 cacgaaacaa aggatcggcc ctttcatctt ctctttcagc ttccgcaccc ccaggtattc
     3781 caggatgcgt tctttgacct tttccaggtc atagtggtcc tcgtccaaaa cctgtttggc
     3841 cttcttgatg tccagattgt cttcggtgct gaccgcccag ggaagctcca ccagccagtc
     3901 caggtaggtc cgcacgatgg tcgcctcggc cgactcgggg tgcatctgct ccagacgatt
     3961 taattgctta ttggcctctt tctccacctc ggaaggcatc ttagctttct tgatcttttc
     4021 ccgcaattct ccgatttcct gagccttttc atcgatctcg cccagctcgg ctttgatcgc
     4081 cttcagctgc tcccgcagga agtattcccg ttgggtcttg ctcatctcct cccgggcttg
     4141 ggactgaatt ttggcctgca tggtcgagac ttggacttct ttggagagaa attcgttgac
     4201 cttcttcagc cgctgcagcg ggttgaaggt ctccagaatt ttctgcgcct cttcgatctt
     4261 gagcttgagg ttggaggcca ccaagtccgc cagtcgaccc ggctcttcga tctggtcgag
     4321 aatggccagc acatcgggag aaaggattcc tttcaaggat agaaccttct cgctctgctc
     4381 tttcacgctg cgaatgaggg cttccgtctc cagagacgtc tccccgacgg aaggctcgat
     4441 gaccctttga atccggacca caaagagggg cttctcctgg atgtattccc ggatggttcc
     4501 tttggccagc ccctgaacca ggatcttcac ccgaccgtcg ggcagtttga gcatcttcat
     4561 gaccatggcc gcggtaccca ccgagtagat gctttccggc gtagggttgt cgtcggtgga
     4621 gaccttctgg gtagccagga agatcatccg gtctttggcc aaggcttcat cgacggcctt
     4681 gatggaactc tcccgcccga cgaaaagcgg cagaatcata tacgggaaaa tcaccgtatc
     4741 ccggacggga agcattggca gcatttccgg aactttcatt tgttcatctt caagaatatt
     4801 ggccattctt tcctcccaat ctattgccac ctcgaaaggt tagggacgca gctaggatca
     4861 attaaattat aatacctgcc ttcccatccg tcaacggtga aggtacctaa gtatcgatgc
     4921 tccttgaaaa atcctttgga gtgaggagcg gagaaaagcg cgggggatca agaattttat
     4981 cgaccgccct ggaggctttc tttaatgaat ccctcttatc ggtgaaaagg ggcttttttg
     5041 ccagtcatgc cctccgccag aggcggaggc ttgaaaaacc cgtgaaccgc tccatcccgc
     5101 aggcaatttc cggccctctt caggaatttc ggaagaagcg gtcgatggcc tccggttcgt
     5161 gggcgattac ccaggggggc aggctttgca gaaagacctt tccatatcct cgggtgagaa
     5221 ggcgggtatc caggatggcc aggatacccc tgtcctggcg ggtgcggatc aatcttccca
     5281 gtccctgctt gagaagcagg atcgccgaag gaacctgata gtcccagaaa ggctttcccc
     5341 cggcggcggc gatccgttcc agcctcgctt ctaggatggg ttccgtggga ggagaaaagg
     5401 gaagtcggtc gatcaccaca cagctgagcg cttccccctg tatgtccact ccttcccaga
     5461 agctggcggt ggcgaaaagg accgagtgga ggtcgtcctt gaaggctttg agaagagccg
     5521 ttttcggccg ttccccctgc aagaaacagg taaaagggac ccgttccttg aggcgccgat
     5581 aggcttcctc catgttccga atgctggtga agaggagaaa agctcttccc aaggaaagcc
     5641 gaagaatttt ttccatctcc tccaccgccc ggggcaagaa tgaagggctg ttgggttcgg
     5701 gcaggtgggg aggcagatag agaagggatt gagccttcag gtcgaaagag gagtctagaa
     5761 tcaactcgtc ggtcccctct cccccacccc cggtcaatcc catgcgtgac ctaaagaagt
     5821 taaaattccc ctgagtggaa agggtcgccg aggtgaaaac ggcggtcttg aggcggggat
     5881 acaggttctc ctggagcgca gacgagacat ccaccggaga gccatggagg aaaacccccc
     5941 ggccccggac ttcaccccag tacaccatct cttcgcgggc gagggatatg aattcttgga
     6001 attccagctt caactcttcc gcccttcgcc cacaagcccg caggccttcc gaaggttcct
     6061 gcatacccgc tatgtgggag gaaagggagg tcaatttatc catcacccgg ccgccggtct
     6121 ccttcgtatc ctctttccat cgttcttttt gcagggggaa acgcgcttcc tttccccgaa
     6181 gaggctggaa gaatctttct tccaggtcca gcagatcggt cgctatccgg gttagagtcc
     6241 cttccttcac cttggctgcg gtgcattccc gccggaggtc cctcgccagt tcttcaaacc
     6301 ggtagttgct gatcgtgctt cccaggtact gggtggctgt ctcctccagc tgatgggctt
     6361 cgtcgaaaac cactgcctca taccggggga ggacctcgcc gtatcccttc aagcgcaccg
     6421 ccagatcggc gaaaaaaaga tggtgattga caatcagaat atcggccccg gccgcttcct
     6481 gtcgcatgac catgataaaa caagggtcat gaaactcaca gctctgacct agacaggttt
     6541 ccgaggatgc gcagacctct ttccataggc ctaggtcttc gggcaggtca gtcagctcag
     6601 cccgatctcc ggtctttgtc tttccggccc acttcttcac agtttggtag tagcccacct
     6661 cttccatgga ggcgaagagc ggctggcggg aaaaaagccg gaaccgcctc cagcacaggt
     6721 agttgcccct tcctttcata aaggccgcgc ggaaggagaa cctgagcttg tcctgcacga
     6781 ggggaacatc cttgaagaaa agctgttctt gaagagtctt ggtccctgtg gaaacgacca
     6841 ctcgtttccc gctgagaact gaagggatga ggtaggccaa ggtctttccg gttcccgtac
     6901 ccgcctcaac gatcagaaaa cgttcctgag ccatagctcg ctggacagct ccagccatct
     6961 gttcctgttc cggacggtac tcataaaaag ggatattttt ggccaagagc ccggatgggc
     7021 tgaagatgtc cttcatcgga agtccccgag gtgcaggaaa atattattaa agaaagaata
     7081 catgacaaag cattccacgg caaagggggg aaaccccagg taccccagga ccggcatctc
     7141 gaacaccttg atatccccca aaatgggaac agtgtaatac caccgggctc cggcccaaaa
     7201 attccagaac tcccacaaaa agccacaagc cgccccggag agaagtaaac tgtaaagccg
     7261 cccagggtac cctttttgca ggtccagaag gagggaaccc cggcccatcc agtagttaag
     7321 gggatcgagg agaaaaataa agcccaccca gaccggagca gccagataat gggcaatcgg
     7381 ggatggggta atcagcggga ggatcagaca caggcccccg aaggccaggg aaaaaagcaa
     7441 ggccatgggg ctgaccttcc aggggcggcc cttggaaagg gaaattcttt tccatcctcg
     7501 catggcttcc gccgtcgtca ggatggctgg ccaaattgta gcgaaagccc aggcatagcc
     7561 caggagacga agggaaacct cttccgggag acccacgtag tgccagttcc gcaggtagag
     7621 gttgtagaac tcaaaaatga gccaaaagcc gatggaaacc ggaaggagga gcagaagttt
     7681 cttgggccga ttcaggaccc acgaggttcc ccggtggaga tggaccaggc tgtccacgaa
     7741 aaggatatag ccgctccaga cgagcggggt gaagaaggtg cgaaccaagg aaaccccgga
     7801 aaaaagcagg atttccgaaa ttccgattat ggccaacccg atccaaccgt gaatcttaag
     7861 cccgggtaca cctttggctc ccctcgtcat tcgtccggtg tccgttgtcg gtagtcgatg
     7921 atcattgatc attggaaatt gctcattggt caatgccctc gctccttcac ggtgtggcgt
     7981 agcaataaag gcacccatgc ctgcacttca gagagtacca tccgatgtcc agactctggg
     8041 tacagccgca aagcgggcgt tgacctctag ccttttcccg ggagcagctc aggccatccg
     8101 ggtggattcg gctcaggagt tcaccatcga tgcatcggga acggggaagt ccttccatgg
     8161 agcagaaaaa aagctccatt ccataagtct tcgcaatctt tttcagccct tccacctgct
     8221 tcttccggtt ttccaggtct tcggagatca gggtccaacc tctcccggtt agacgcctca
     8281 ggactttctt gtacggagac acccaactca cccggcatgt attaattccc catggagcaa
     8341 tggctgcgca gagttgggga aagctgtgaa ggttgctgta ggttccttcc ctgccttcta
     8401 cttccaaaat ggggtcgaac cgccaggaaa ttcgccccgg gtccttcacc atctccacca
     8461 gcggcgcgat catccccaca gtttcttccc aggggggaat cagcggctca aattcccctc
     8521 cccccatccc ggtgatcgtg aggtggagat aaatctgcct gtatcttttg agggtttctt
     8581 ttagttcccc ctcgatcacc aggttccggg gattcttggt ccagatcacc aaagaatgaa
     8641 cggattcggg aggatattcg cgaagacgct cccccaggac ctttggaaag cagcccacca
     8701 ggtctgtcct gcggctggca gataggacga gaagattttt catccaagag attccttaag
     8761 gaatgagtct atcttacgag agtggtcacg cttcgtcaat acgttcggtt ttttggagtg
     8821 ccggaaaggt gggaaaggaa ggcaggtccg ccttggactt tacctttgtc gagtcagagc
     8881 ctacgcttgc agaataattt acagcccgga gagcccaccg caacccaaag aagtgcttga
     8941 accttcttcc agcttcaagc ctggagacct tcgggagaca cggcagcctc ctcggaagac
     9001 gtggagatgc cgtatttgtc cattttatac ttcagaatcc gccggctgat tcccaggagg
     9061 tccgccgcct tggtctgaat atagcggctt ttcttaaggg cttcgaggat aatatcccgc
     9121 tcgaaatccc gttccgcatc ttcgaaggaa atccgaccct ccaggactcc ttgcttgata
     9181 tgctccgtcc gggaggggct cttcaggctg accggcagat cgttgggcgt cacgatatca
     9241 gtgcagctga ggaccacgat ccgttcgatg atattttcca gctcccgcac gttgccgggc
     9301 caatcgtagg ccgcaaggat ttccatggcc tccatggaga ttttctgctt gccgggtcca
     9361 gcatgctttt cgagaaaatg ttgaatcaag agggggatgt cctccttccg ttggcgaagg
     9421 gggggaatgg tcaaagggac cacatttatg cgatagtaaa ggtccgagcg gaacgatccc
     9481 cggcccatca tctcttccaa gttcttgttg gtcgccgaga tcaggcgcac atccacctgg
     9541 attgttttgg tgccccccac acggacgaat tcccgctcct gcagggagcg gaggattttg
     9601 gcctgggtgg gcaggctgag ctccgagact tcatccagaa aaagggttcc ctgatgggcc
     9661 agctcgaaat gaccgatgcg ccggttctgg gcgtcggtga aagccccttt ttcatggccg
     9721 aagagctcgc tttcgatcaa agtttccgga atggccgcac agttgaccgc cacgaagggt
     9781 ttgtttttcc tggggctgtt gtagtgaatg gctcgggcca ccagctcttt tccggtcccg
     9841 ctttctccat ggatcaggac cgtggaattc cgctccgcga tctgcgcgat ggtagcgtag
     9901 atctcccgca tctctttgga tttgccgatg atattgtcaa accggtatcg ttcttcgatc
     9961 tccgtctgca gccgtcggtt ctccgccttc aagtcccggc tctccagggc cttgtcggcg
    10021 atgatcttca actcctcgac gtcgaaaggc ttggtgatgt agtcataggc ccccagtttc
    10081 atggcctcca ccgccgtttt caggtttttg gcggcggtga gcatcaccac cggggtgagg
    10141 ggttggctct cccggatttc tttcaacagc tccatgccgt ccttctgggg catgatgatg
    10201 tccaaaaaga tgaggtcggg ctcctcgctc aggatcttct cccaggcttc tcttgcgctt
    10261 cccgcagtga cgacctgata atccttcttg aagatcatgc gcagcgagtc acgaacgctc
    10321 aattcatcat ctaccgccaa gatctttcgc atggttatcc tttaaccgcc ctccgcctca
    10381 catccccgcc ggaaactgga tgcggagggt catccctccc tcttcctcca gcagaacctg
    10441 catctttccc cgattgcgca gcatgacttt gcgggtcaac aaaagggcta ggttcaggtt
    10501 ttgaaattcc ggtttggttt cgaaaccgac gacccgggat aaattgccca ggaccccttc
    10561 ggggctattg taccacagaa tcatatcgac ccaatccggt ctaccttccg ccccctcttc
    10621 ccgagtgaaa tgcgtggtga tatggagcgg aaaattatcg ttccctttga agaggacgtg
    10681 ttcgaaaagg ttccgcagga caaaattcaa ttgcgtctta tcgaagagaa tcgggggcaa
    10741 ggcatttccc aggtcggcga cgattttccg ctctgcctgt cccgggaact tcccctgttt
    10801 tcccaaaaac tcttccaggg tcgcattcag gtcctggagg ctggattggg gggtagaaaa
    10861 ctgggcgaac tccaggagat tttcaaaaag ggcattgatc cgccgcacat cctgattgac
    10921 caaacgggag aattcctggc ggaactcagg gtcttcatac ttgtcgggca gaaggtgggc
    10981 gaaggtactg atagccacca agggattctt gatctcatga gccagggtag gaatggtcac
    11041 atcgaaccag gaatcctttt catcgccgca aggagtctcc cggagcgagc ggccgcggga
    11101 aggactgaag gccaggtctg cgggttctat caaatttccc tctttcagga aagccgaccg
    11161 caggaccagg ctttccagtt ccgccaaatt tccaggccag tcatactctt tcagggcttg
    11221 gagggcctcg ggggaaaaga tcttgctccc gcccctttcc cccgctttct cccgcaaaat
    11281 ttcctgaacc atccggggaa ggtcttcctt ccgttcgcga agcggcggta ggcgaagggt
    11341 cagtatctcc agggtttgca aaaggtcttc ccgaaatttt ccggcggtca cgggttccgt
    11401 catgtcgcca gaggaagaag aaaggacctg aacgtcaaat gtggtttcct gcaggccggg
    11461 ccaaggaatt tggcggctgc tgagaaggtc ctgcaggagg gcttgcatct cctcctccag
    11521 gctctccaaa ttttcgagga aaagagtcaa agagagtttt ggactcattt ttctttcttt
    11581 tccccagagg aggattttct ctacgaaact ctccggggtt aatccccggc agggaaagtg
    11641 aagaaagggg ctcatcttcc agggaccgag aaaatgaagg acccgggcca agtcgccttt
    11701 ccccgtccct tcctctccct gaatgaacac cggaagcctg gatgaggc
//
LOCUS       DASPRR010000613         9095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2399811, whole genome shotgun sequence.
ACCESSION   DASPRR010000613 DASPRR010000000
VERSION     DASPRR010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9095)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399811"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..721)
                     /locus_tag="VEL68_22565"
     CDS             complement(194..721)
                     /locus_tag="VEL68_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXZ38829.1"
                     /translation="MEERTIEPKRGYVWLGILAILFGAMAYLYYFYPERNIGPQQPIY
                     FSHRVHAGVKEIPCRFCHPGVERSPNAGIPPMEKCFFCHEYIIPQHPQIVKEKEHFDS
                     GQPVPWVRIYWVPDFVFFNHIPHIKWAKLDCTECHGAVKTLDRLRPVNFQMGFCLDCH
                     RKRGAQVDCWLACHR"
     gene            complement(745..1962)
                     /gene="nrfD"
                     /locus_tag="VEL68_22570"
     CDS             complement(745..1962)
                     /gene="nrfD"
                     /locus_tag="VEL68_22570"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXZ38830.1"
                     /translation="MSPAEPSRSAQIPRGGMILFAALALAGAAAFLLGIGGPQSLRAW
                     QAYLVNYVFWTGLSFGAVLFAAVLNMTDARWGRSLKRLAEGLGAFLPASFLLFWVLYF
                     GREEIFPWIREPSTERSGWLSVGFLFARNGAGLLLLTLLSMTLISNSVKADRQFINRP
                     PGTSDPAGGKPWKAQRVLSPIIGILYAFILSLLAFDLIMSLDPHWYSTLFGAYYFMSS
                     FFAGLAALVFVSHLLRRACGLQEYIHPHHLHDLGKLLFAFCLFTGYLFYVQFLVIWYG
                     NLPEETKYIILRVRFTPWEPLGWTVLGMIFILPFLVLLNRKWKMNPAVMIPVSLVVLV
                     GMWLERFILVAPSLWKEKSLPLGFMEVLITAGFFGIMALSLAIFLQRAPLLPVSDPFF
                     REAMQPHEEKERP"
     gene            complement(1949..2461)
                     /locus_tag="VEL68_22575"
     CDS             complement(1949..2461)
                     /locus_tag="VEL68_22575"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HXZ38831.1"
                     /translation="MVPDPALLGIFPYLDDLLDALRKAKKEGFKILTVFSPIHFSEIQ
                     EILGHKPSTVRYLCLAGGILGGTSLVGLAVYAHLSFKLITSGKPVLPWIPFVIVFFEG
                     TVLLAVIFAVAAWILKGRLPRVRLHAAYDPRFSEDRFGILLGYQGEEQEAVRRLLMEA
                     GAEEIRDVAG"
     gene            complement(2451..3779)
                     /gene="nrfD"
                     /locus_tag="VEL68_22580"
     CDS             complement(2451..3779)
                     /gene="nrfD"
                     /locus_tag="VEL68_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXZ38832.1"
                     /translation="MTFQDVNREILRTLSPPTRSYYLIVGLLLLGILGGACVWGYQIA
                     VGLGVTGLKHPTMWILYLVNFVFWVGIAHSGTLISAILYLFRAKWRTPIARSAEAMTV
                     FAVMIAGFFVFIHLGRVWILYWIIPYPNQRTLWPDFQSPLVFDFLAVSTYLTVSVLFW
                     YTGLVPDLAAVRDRSQGIRRKIYGFFALGWTGTDRQWHHYGTAYLLLAGLATPLVVSV
                     HSVVSWDFALAVIPGYHSTIFAPYFVAGAIHSGLAMVLTLLIPLRRIFHFEKIITARH
                     LESVAKTTILTGLIVGYSYLVEYFMAWYSGNIFERQTFLFRAFGHHWFMFWLMVFCNA
                     VVPLLFFFKKIRTSFFWLFLIAVLINIGMWSERFVIIIGSMSQDFLPHNWGFYTPRWV
                     ELSITVASFCLFLFLFLLFAKFLPSISIAEIKEDLTPLIRGKGKRDHHGA"
     gene            complement(3811..6768)
                     /locus_tag="VEL68_22585"
     CDS             complement(3811..6768)
                     /locus_tag="VEL68_22585"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXZ38833.1"
                     /translation="MSEFSRRNFLKVVTLAGTAAAAGCSSEPVRKLIPYIIPPEDIVP
                     GEATWYATTCRECPAGCGLLAKNRDGRIIKVEGNPLHPVNKGKVCPRGQASVQGVYHP
                     DRYRGPQRKNPQGNWESISWEAGEEFLLAKLRDLKEKGRTERLVFLSELITGVQRDLI
                     GRWLAELGAGGPLLYEPLAYEPLRQANLSVFGQDRIPTYRIDRADFLLSFGANFLEAW
                     LSDVQYARQFASFRTPGEKGKNLFVYVGPRYSMTAANADNWIAVSPENMSLVASGLLR
                     ILLPKSSSPSLRGGLRSSLEGKLKDFSPEAVQNRTGVKAETLQDLARRISASRRPLVL
                     SSGLTFGDPKAADTAAAANLLNLVSPGSSALLDFEDSSSLGNTVRAEKMKELTQRMLQ
                     GEVEILLISRANPVFHLPPSWQFQKALQKVPLVVSFSSLPDETSELAHLILPSPTFLE
                     AWGDYQPRTSVEGLMQPVMGPMFDTRPVEDLLIAAGKKLAGEKKFPWKNVYEMLRQAW
                     AEKAKKPGAAEASWQESLQRGGTWGSSPQPASKSLVAVAGLNFSPSVPAAQKEGKLFQ
                     FVTYPTIQFFDGRSSSRPFLQELPDPLTEITWGGWVEINPRTARELGIEKGDLLNLRS
                     SNGIVKAPAFPYPGIPAGVLAMPLGQGHAAGGLFTPGESENPIRLISPELDMSYKGLR
                     WALLEVTLEKAGGSIPLANVDGSFYQHGRNLAQAISWQEYQERKSSGKKPAIRLPLPE
                     GFSQKDDFYQPHQHVDYRWVMIVDLDRCIGCGACVVACYAENNVAVVGRKRVLDGREM
                     SWLRIERYFDWDRIRAQFLPMLCQHCDEAPCESVCPIFAPNHSKEGINNQVYNRCIGT
                     RFCSQNCPYKVRRFNWFTYERYEPLNWQLNPDVTVRHKGVMEKCSFCIQRIVEAKVRA
                     RSEGRKVRDGEFTTACAQTCPAGALIFGNLLEPESRVSKLIQDPRAYQVLEQLNTKPA
                     VIYLKKIFPEGKI"
     gene            6957..7853
                     /locus_tag="VEL68_22590"
     CDS             6957..7853
                     /locus_tag="VEL68_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXZ38834.1"
                     /translation="MKLFLALSRTPHGLLDLAAPAMSALLWLGGFPPVEILALGLITA
                     FSGYTAVYALNDVVDRRVDKEKMQGSRLPRAQKDLDSVFVRHPLAQGMLSSREGLFWT
                     LAWGALALVGTYLLNPICTVIFFLACLLEYSYCRLLKISYFRGIISGLVKTSGPLAAV
                     FAIDPNPDPGFLATLFAWLFLWEIGGQNVPNDFSDMEEDRKIGAKTIPVKFGAQGSIL
                     IIFTSLFLVLGMSLVMIWVAPKGLNLAYLFGALLSGVYFLLIPGCRLFQSKNPQDAFI
                     LFNRASYYPLAILLMTVMSWFF"
     gene            7862..8575
                     /locus_tag="VEL68_22595"
     CDS             7862..8575
                     /locus_tag="VEL68_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HXZ38835.1"
                     /translation="MKELDRESLKEFNGKDGKPVYVAFQGRVIDVTNSKFWKTGVHMK
                     RHPSATDLTIDIEAAPHGPEVLDRYPQVAVLKEKEETGRPMPKWLEGLLGRFPILRRH
                     PHPMVVHFPIVFSFTPGLFLSLFLITGYRPFEATALHCLGGGIFFTPVAILTGYFTWW
                     LNYLAKPMRPVTIKIRFSTLLWGFFILAFIYRILNPEILLSLSGGSMLYILLVFSLIP
                     FATVVGWFGATLTFPVEKK"
BASE COUNT         2053 a         2446 c         2655 g         1941 t
ORIGIN      
        1 cctcttacca ctcttgcctc aggggtggta ggcaattttc ctgaaagcct aatgatccga
       61 atagcgaagt ccccggtacg tttttctaat tttctcccaa attccttatt atccattttt
      121 gacaattccg aactctaagc actctgggca ctcgaaactc taggcactct aggcacttcg
      181 aattctaggc acttcatctg tgacacgcca gccagcaatc cacttgtgct cccctcttcc
      241 gatgacagtc gaggcaaaac cccatctgaa aattgactgg ccggaggcgg tccagggttt
      301 tgaccgcgcc gtggcattcg gtgcagtcga gtttggccca cttgatatgg gggatgtggt
      361 tgaagaagac gaagtccgga acccagtaga tccggaccca gggaaccggc tgtccgctat
      421 cgaaatgctc cttttccttc acgatctggg ggtgctgggg aataatgtac tcatgacaaa
      481 agaaacactt ttccatcggg ggaatccccg cgttggggga acgctccacg cctggatggc
      541 aaaaccggca gggaatctcc ttcacccccg catggacccg gtggctgaag taaatgggct
      601 gctgggggcc gatgttccgc tctgggtaga aataatataa gtaagccatg gcgccaaaga
      661 ggatggccag aatccccaac cagacataac cgcgcttagg ctcgatcgta cgctcttcca
      721 ttgtcttcct tcaaaaagaa ttcatcaagg cctctccttc tcttcgtggg gctgcatggc
      781 ctcccggaag aaaggatcgg acaccggcag aaggggtgct ctttggagaa agatggccag
      841 gctcaaagcc ataatcccga aaaaaccagc ggtgatgaga acttccatga aacccagagg
      901 aaggcttttc tccttccaga gagaaggcgc caccaggatg aaccgctcca gccacatccc
      961 cactaggaca accaagctga ccgggatcat gaccgccggg ttcatcttcc atttcctgtt
     1021 caggaggacc aggaagggga ggatgaagat catacccaga accgtccagc ccagcggttc
     1081 ccagggggta aagcggaccc gcaggatgat gtacttagtc tcctcgggga gattgccata
     1141 ccagatgacc aggaactgga cgtagaaaag atagccggtg aagaggcaga aggcaaagag
     1201 gagtttcccc aggtcgtgga gatggtgcgg atggatgtat tcctgaaggc cgcacgccct
     1261 ccggagcaga tgagaaacga aaacgagagc ggcgagaccg gcgaaaaagc tggacatgaa
     1321 gtagtaggct ccgaagagag tgctgtacca gtgggggtca agggacatga tgaggtcgaa
     1381 ggccagaagg ctcaggatga aggcgtagag gatgccgatg atcggggaga ggacccgctg
     1441 ggctttccag ggtttccctc ccgccgggtc ggaggttccg ggcgggcggt tgatgaattg
     1501 ccggtcggcc ttcacggaat tagagatcag ggtcatggag agaagggtta agaggaggag
     1561 cccggccccg ttgcgggcga agagaaagcc gacgctcagc cagccggacc tctccgtgga
     1621 aggttcccgt atccagggaa agatctcctc ccggccgaag tacaggaccc agaacaggag
     1681 gaaggaggcc ggaaggaaag cccccagccc ttccgccagc cgcttcaggg agcggcccca
     1741 ccgcgcgtcg gtcatgttca acaccgccgc gaagagaacc gccccgaagg aaaggccggt
     1801 ccagaagaca taattgacga gataggcctg ccaggctctg agggattgag ggccgccgat
     1861 ccccaggaga aaggcggccg ctcctgccag ggcaagggcg gcgaagagga tcattccgcc
     1921 gcgggggatt tgggcagacc tgctgggttc agccggcgac atcacgaatc tcctccgctc
     1981 ccgcttccat caggagtctc cggacggctt cctgctcttc tccctggtac cccagaagaa
     2041 ttccaaagcg gtcctcggaa aaacggggat cataggccgc atggagacgg acccggggca
     2101 gccggccttt caagatccat gctgccacgg caaagatcac ggctagaagg accgttccct
     2161 cgaagaagac gatcacgaaa ggaatccagg gaagaacggg cttcccgctg gtgatcaact
     2221 tgaagctcaa gtgggcatag acggccaggc ccaccaggct cgttcctccc agaattcccc
     2281 cggcgaggca gaggtatctc accgtgctgg gtttgtgccc caggatctcc tggatctcgg
     2341 aaaagtggat gggggagaag accgtgagga tcttgaagcc ttccttcttc gcttttctca
     2401 aggcgtccaa gaggtcatcg agataaggaa aaatgcccaa cagggcaggg tcaggcacca
     2461 tggtggtctc ttttcccctt tccccggatg aggggggtaa ggtcttcctt gatttcggcg
     2521 atggagatgg acgggaggaa tttcgcaaaa agaagaaaga gaaacaaaaa gaggcagaag
     2581 gatgccacgg taatgctgag ctcgacccag cggggggtgt aaaaccccca attgtggggg
     2641 aggaagtcct gcgacataga tccgatgatg ataacgaacc gttccgacca catgccgatg
     2701 ttgatgagca cggcgatcag gaacagccag aagaagctgg tgcggatctt cttgaagaag
     2761 aagaggagcg ggacaaccgc gttgcagaac accatcagcc agaacataaa ccagtgatgg
     2821 ccgaaggccc ggaagaggaa cgtctgcctt tcgaagatgt tcccgctgta ccaggccatg
     2881 aaatactcga cgaggtagga atagcccacg atgagcccgg tcaggatcgt ggtcttcgcg
     2941 acggattcga ggtgccgggc ggtgatgatt ttctcgaaat ggaaaatacg ccgcagggga
     3001 atcagaaggg tcaggaccat ggccaggccg gaatgaatcg ccccggctac gaaatagggg
     3061 gcaaagatcg tggagtgata tccggggatg acggccaaag caaagtccca ggagaccacg
     3121 ctgtgcacgg agacgaccag gggcgtagcc agtccggcga gaagaaggta ggcggtcccg
     3181 tagtgatgcc attgccggtc ggtgccggtc cagcccaggg cgaagaagcc gtagatcttc
     3241 cggcggattc cctgggaccg gtcccgcacc gccgccaggt ccggaacgag tccggtgtac
     3301 cagaaaagga cgctcaccgt gagataggtg gaaacggcga gaaaatcaaa gaccagggga
     3361 gattggaagt cgggccaaag ggtccgctgg ttggggtaag ggatgatcca gtacaggatc
     3421 cagacccgcc cgaggtggat gaatacgaag aacccggcga tcatcacggc aaagaccgtc
     3481 atggcctcgg cgctccgggc gatgggcgtt cgccacttcg cccggaagag gtagaggatg
     3541 gcggagatca acgtgcccga gtgggcgatc ccgacccaga aaacaaaatt gaccaggtaa
     3601 agaatccaca tggtcgggtg cttgagcccc gtgaccccca gtccaaccgc gatctgatag
     3661 ccccacacgc aggcgccccc caggattcct aggagcaaga gccctacgat aaggtaatag
     3721 cttcgggtag gaggggacaa ggtccggagg atttcccggt tcacatcctg aaaggtcatc
     3781 ttctccaaaa ttcaatcctc gcacttacct tcagattttt ccttccggga agatcttctt
     3841 taaatagatt accgcgggtt tggtattcag ctgctccagc acctggtatg cccgggggtc
     3901 ctggatgagc ttggaaaccc ggctttccgg ctccagcaag ttgccgaaga tcagtgcccc
     3961 tgcggggcag gtctgagcgc aggcggtggt gaattcgccg tcccggacct tccggccttc
     4021 gctccgggcg cgaaccttgg cttccacgat cctctggatg cagaaggagc acttttccat
     4081 caccccttta tgccggaccg tcacatcggg attcagctgc cagtttagcg gctcgtagcg
     4141 ctcgtaggta aaccagttga agcggcgaac cttatagggg cagttctggg agcagaagcg
     4201 cgtgccgatg cagcgattgt aaacctggtt gttgatccct tccttgctat ggttgggggc
     4261 aaagatcgga cagaccgatt cacaaggagc ttcatcgcaa tgctggcaga gcatggggag
     4321 gaattgggcg cgtatccggt cccagtcgaa atagcgctcg atgcggagcc aggacatttc
     4381 ccgcccgtcg aggacccgct ttctccccac cacggccacg ttattttccg catagcaggc
     4441 gacgacgcag gcgccgcagc cgatgcaacg gtccaagtcc acgatcatga cccagcggta
     4501 atccacatgc tggtggggct ggtagaagtc atccttttgg gaaaaccctt ccgggagtgg
     4561 caatcgaatg gcaggttttt tcccggatga tttccgttcc tgatactctt gccaggagat
     4621 cgcctgagcg agatttctcc cgtgctgata gaagcttccg tccacgttgg ccaggggaat
     4681 ggagcctccg gccttttcca gagttacttc caaaagagcc cagcgtaatc ccttgtagga
     4741 catatccagt tctggagaaa ttagcctgat ggggttttcc gattcgcccg gagtgaatag
     4801 accgccggca gcatgcccct gaccgagcgg catggctagc accccggcgg gaatgcccgg
     4861 gtaggggaaa gccggggctt tcacgatccc gttagaagat cgaagattca agaggtctcc
     4921 cttctcgatt cctagctccc gggccgttcg ggggttaatt tccacccagc ctccccaggt
     4981 gatctcagtc aggggatcgg gaagctcctg caggaagggc ctgctagagc tccggccgtc
     5041 gaagaactgg atcgtcgggt aggttacgaa ttgaaaaagt tttccttcct tttgagccgc
     5101 cgggacagaa ggagagaaat ttaggccggc gacagctacc agggacttgg aagcgggctg
     5161 gggggaagaa ccccaggtac cccctctttg caggctttcc tgccaggacg cttcggccgc
     5221 acctggcttc ttggcttttt ccgcccaggc ctgtcgaagc atctcataca cgttcttcca
     5281 tggaaacttt ttctccccgg ccagcttctt ccctgcggca atgagaaggt cttccaccgg
     5341 ccgggtgtcg aacatggggc ccatgactgg ctgcatcagg ccttccacac tggtgcgtgg
     5401 ctgatagtcc ccccaggctt ccaggaaagt aggagagggc aggatgagat gagcgagctc
     5461 gctggtttca tcgggaagac tggaaaagct gaccaccagg ggaaccttct ggagtgcctt
     5521 ctgaaattgc caggagggag gaagatgaaa aaccgggttg gcgcggctga tgagaaggat
     5581 ctccacctct ccctggagca ttctctgggt caactccttc atcttttccg cccggaccgt
     5641 atttcccaag gaggaggagt cttcgaaatc caggagggcg gaggagccag gggagacgag
     5701 gttaagaagg ttggccgccg cagccgtgtc tgccgccttc gggtctccga aggtgagccc
     5761 cgaggacagg accaggggcc tccgggacgc ggatattctc cgcgccaggt cttggagcgt
     5821 ctccgccttc acaccggtgc ggttttgcac ggcttccgga gagaaatctt tgagctttcc
     5881 ttccagggaa gacctcagcc ctccccggag ggaaggggac gaacttttcg gcagaaggat
     5941 tcggaggagg cccgaggcca ctaaagacat attctccggt gagacggcaa tccagttgtc
     6001 cgcgttggcc gcagtcatcg agtagcgggg gcccacatag acaaaaagat ttttcccctt
     6061 ttctcccggg gttcgaaaag aggcgaactg ccgggcgtat tggacgtcgg acagccaggc
     6121 ctcgaggaag ttggcgccga aggaaaggag gaagtcggcc cggtcgatcc ggtatgtggg
     6181 aattcggtcc tgtccgaaaa cacttagatt ggcctggcgc aggggttcgt aggccaaggg
     6241 ctcgtagagg aggggaccgc cggcgcccag ctctgccagc catcgcccga tgaggtccct
     6301 ctgaacgccg gtgatgagtt cactcaagaa gaccaggcgc tcggtccttc ctttttcttt
     6361 caagtcgcgg agcttggcca ggaggaattc ttcccccgcc tcccaggaga tcgattccca
     6421 attcccctgc gggttttttc tttgaggccc gcggtatcgg tccggatggt agaccccttg
     6481 aaccgacgcc tgtccccggg ggcacacctt tcctttgttc acggggtgaa gaggattccc
     6541 ctcgactttg atgatccgcc cgtcgcggtt cttggccaga aggccgcagc ctgccgggca
     6601 ttcccggcag gtggtggcgt accaggtcgc ctccccgggg acgatatcct ccggggggat
     6661 gatgtagggg atgagcttcc gtacgggttc cgaggagcac cctgcggcgg ctgcggtccc
     6721 ggcgagagta acgactttca ggaagttccg gcgggagaat tcgctcataa agaggcccct
     6781 aagaaagatt ataaggaaat tgggtcgaaa gtcttcaagt ttttttcaag aaatttttgg
     6841 cccgaatttt catttgattt tggcccgaaa aaaatgagaa gataggaaca ctcgtccatc
     6901 caaagggaga tgctttgaaa accgcttcgc ttcagagccc aatgggagtt tcccggttga
     6961 agctatttct cgccctttcc cggacacccc atggactcct ggacctggcc gctccagcca
     7021 tgagcgccct gctctggctg ggtggttttc cacccgtcga gatattggcc ttgggcctga
     7081 taaccgcctt ctccggctac acagcagtct atgcgctgaa tgatgtggtg gataggcggg
     7141 tggacaagga gaagatgcag gggtccaggc ttcccagagc ccaaaaggat ttggacagcg
     7201 tcttcgtccg ccaccccttg gcccagggga tgttgagctc gcgagaaggt cttttttgga
     7261 ccctggcctg gggggccttg gccctggtgg gaacctatct cttaaatccg atctgcaccg
     7321 tcattttttt cctcgcctgt cttttggaat acagctactg tcgactcttg aaaataagct
     7381 attttcgggg aatcatcagc ggactggtca agacctcggg ccctctcgcc gcggtttttg
     7441 ccatcgatcc gaaccccgac ccgggattct tggctacgct tttcgcctgg cttttcttat
     7501 gggagatcgg ggggcagaat gtgccgaatg atttttcgga catggaagag gatcgaaaaa
     7561 tcggagctaa aacgatcccg gtgaaatttg gcgcccaagg ctcgatcctg atcattttca
     7621 cctctctttt tctcgttctg gggatgagct tggtcatgat ttgggttgcg cccaaaggat
     7681 taaaccttgc ctatctcttc ggggctttac tatccggtgt atattttctg cttattcccg
     7741 ggtgcaggct ctttcagtcg aagaatcccc aggatgcttt tatcctgttc aaccgggcta
     7801 gctattaccc tctggccatt ctcctgatga ccgtgatgag ctggttcttt tagaggaatc
     7861 catgaaagaa ttggatcggg agagtctgaa ggaattcaac ggcaaggatg ggaagcccgt
     7921 ctatgtggct tttcaggggc gggtgattga tgttaccaac agcaagttct ggaagaccgg
     7981 cgtgcacatg aagcgccacc cgtccgcgac cgacttaacc atcgacattg aagcggcgcc
     8041 ccatggacct gaagttctgg accggtaccc tcaagtggcg gttttgaaag agaaggaaga
     8101 aaccggcagg cccatgccga agtggctgga gggccttctg ggccggttcc ccatccttcg
     8161 ccgacatccc caccccatgg tggtccactt cccgattgtg ttttctttca ctccgggtct
     8221 gttcctttcc ctctttctaa tcaccggata cagacccttt gaagccacag cgcttcactg
     8281 tctgggcggg gggatctttt tcacgcccgt ggccatattg actggatact ttacttggtg
     8341 gctgaactat ctggccaaac cgatgagacc cgtcaccatc aaaattcgct tctccaccct
     8401 gctctggggt ttcttcattc tggcctttat ctaccggatt ctgaatcctg aaattctcct
     8461 ttccttaagt ggggggagta tgctatacat cctgctggta ttttccctaa ttcccttcgc
     8521 aaccgtagta ggttggttcg gcgcaactct cacctttcca gtagagaaaa aatgatcgaa
     8581 aaccggggaa ggggggcttt cctgttttct aaagattatg ccaagggcag cgtcagaagt
     8641 gaacgttttt gggcgggaac gggcggtttt tagtaaggga aaacccttca aatagaaaat
     8701 acataactta taataactta tcatctttaa acaggcattt taccttccat ccgtagggaa
     8761 ttcccttcat aaaaaacgtg ccctttaaaa aaatatataa tgaaatcaat atataattta
     8821 actatcgatt cggcatccat attgctccgg aaacagatgg tcaggcaaca aggccagaga
     8881 gccttttggg gtggattcga tttaggcctt caatagcgcc ctcggttgac cgcgattatg
     8941 agggaagcga taaatcggtg agtttgatga caaagccggg gaaatccctt ttcccaattc
     9001 ttctgataaa tcgtttcaaa ttttgccatt ttattttttt ggggtctatc cttctttttt
     9061 ccctgccccc atcaaatgcg cactccgctc aggtc
//
LOCUS       DASPRR010000614         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2086643, whole genome shotgun sequence.
ACCESSION   DASPRR010000614 DASPRR010000000
VERSION     DASPRR010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086643"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1705
                     /locus_tag="VEL68_22600"
     CDS             347..1705
                     /locus_tag="VEL68_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004593065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ38836.1"
                     /translation="MNRDQVRVDIKTPPPGPNARKWAEFHRRYAARSTYYDGFIWDRS
                     KPAIGPFCTDVDGNVILDFVSHVASSGLGYNHPELVSMASRLQSVDPDRYAGCDFIGA
                     WGEDPDKSEVPTPSHLHYKLMEITSQFGFGSAFFSNSGAEAVENAIKICYDHKKNYGY
                     GVCFYGAFHGRTLGALSLNRSKKTQRDWFPQIPNIIDFPYCTCQNHPCRCGWMVCCTG
                     KEGMRSRLDHFLDPDIGLVDPEEVAFIIIEPIQGEGGYNVPSIEFMKEVNRLAKKHSI
                     PLISDEVQAGMGRTGRWWAIEHFGIKPDIITVAKPLRVGATLGRRELFPQQEYRISST
                     WGEGNALSSAIGYRMIEIIQKENLLDNATRMGNYFLEQLRSLQGKYPFIYEVRGIGLM
                     DAVEIDSRERRNRMIRKAFEKGLLLLGCGYKVIRFLPPLDVRRREIDMALEVLEGVCK
                     QV"
     gene            1968..>2695
                     /locus_tag="VEL68_22605"
     CDS             1968..>2695
                     /locus_tag="VEL68_22605"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HXZ38837.1"
                     /translation="MNPRIFREYDIRGLVDQDLTVEGARLIGQGLATYLSRQGKRRVV
                     VGRDGRLSSPRFRDPLVQGMVAGGMEVMDIGVCPTPVFYFSIFHLNRDGGAMITGSHN
                     PPEFNGFKVCSGKDTLHGEQIQDLRRIIESQNFFRGEGSVSQQEVIPAYRQFLGQNIW
                     LQRKIKVVIDSGNGTAGLVAPGILRDLGCEVIDLYTEVDGRFPNHHPDPTIPEYMQAL
                     IAKVKETGAEVGIGYDGDADRIGAV"
BASE COUNT          636 a          731 c          755 g          573 t
ORIGIN      
        1 ggatagtaac cagggttccg gtgaacgcta ttatccatgc gtaatggatc attttttgta
       61 atccagggtg ataagaaagg ttctttccga atacaataca ggaagcgaac gtccttggaa
      121 cgcccccatc tgcaatatac caaaaaatag gtaaagaagc aggcggaagt tgagccaggc
      181 gttgatccac gttcgattct ctgttatttt taaaaaagac agggtggagg cttatctttc
      241 cctcgggtct atcgcggatc ggggaaaaga atcttcggcc cacagaccga agccttcccc
      301 atgctttttc ccctcctttt ccgggggaaa ggagacagga ggaattatga accgggatca
      361 ggttcgcgtg gacattaaaa ccccacctcc aggcccgaat gcccggaagt gggcagaatt
      421 ccaccgccgc tatgcggccc gttccaccta ttacgacggg ttcatctggg accggtccaa
      481 accggctatc ggccccttct gcacggacgt ggacggaaac gtgatcctgg acttcgtctc
      541 ccatgtggcc agctccggcc tgggatacaa tcaccccgag ttggtgagta tggcctccag
      601 gcttcagtct gtcgacccgg accgctatgc gggctgcgac tttatcggcg cttggggcga
      661 ggacccggac aagagtgagg tccctactcc ctcccacctc cattacaagc tcatggagat
      721 cacttctcaa ttcgggttcg gctccgcctt cttctccaac tccggcgccg aagccgtgga
      781 gaatgccatt aaaatttgct acgaccataa aaagaactac ggctacgggg tctgctttta
      841 cggggccttc cacggacgaa ccctgggagc cctttccctg aaccggagca aaaagaccca
      901 gcgggactgg tttccccaga ttcccaatat tatcgatttc ccctactgca cctgccagaa
      961 tcacccctgc cggtgcggct ggatggtctg ctgcacgggg aaggaaggca tgcgaagccg
     1021 tctggatcat ttcctggacc cggacattgg gctggtcgat cccgaggagg ttgccttcat
     1081 catcattgaa cccatccagg gggagggcgg atacaacgtc ccgagcatag agttcatgaa
     1141 agaggtaaac cgcctggcaa agaagcactc tatccccctg atttccgacg aggttcaggc
     1201 cggaatgggc cggaccggaa ggtggtgggc catcgaacat ttcggcatca aaccggacat
     1261 catcaccgtc gccaaacctc ttcgcgtagg ggcaactctg gggcggaggg agctcttccc
     1321 ccaacaggaa taccgaatct ccagcacgtg gggggaggga aacgccttga gctcggccat
     1381 cggctatcgg atgatcgaga tcatccagaa ggagaacctc ctggataacg ccacccggat
     1441 gggaaattat ttcctggaac aactgaggag cctgcagggg aaatacccct tcatctacga
     1501 ggtgcggggg atcggcctga tggatgccgt ggagatcgac tcccgggaga ggagaaaccg
     1561 catgattcgt aaagccttcg aaaagggtct cctgctcctg ggttgcgggt acaaagtcat
     1621 ccgtttcctc cctcctcttg acgtccgaag acgggagatc gatatggccc tggaggtcct
     1681 ggagggggtc tgtaaacagg tttaatgcag gctgcaggac tcggatcgca aatcgcaaat
     1741 taaggatggc aggtcaaaga ctccaagttg caggtctcac ttttcaagtt ataaggaaat
     1801 ggggccgatc atttattgtg cgacctgcgg caggtaatct gtgacttcac cttattgggc
     1861 cattttgttt gaccgtaaag catcggtgtg ttactttaat ttaggatcac cccttggatc
     1921 ttattgaaat tcaaaagaaa agcagcaaat taacgccgaa aaaacccatg aaccccagaa
     1981 tcttcaggga atacgacatc cggggcctgg tcgatcagga tttaactgtc gagggggccc
     2041 gcctgattgg tcaaggcctg gctacttatt tatcccggca ggggaagcga agagttgtgg
     2101 tgggccgcga cggccggtta agctcccccc gctttcgcga ccctctggtg cagggtatgg
     2161 tagcaggagg gatggaagtg atggacattg gggtctgccc cactccggtc ttctattttt
     2221 ctattttcca cctgaaccgg gacggcgggg ccatgatcac cggaagtcac aatccccccg
     2281 aatttaacgg attcaaggtc tgctccggaa aagacaccct ccatggggaa caaatccagg
     2341 atttgcggcg aatcatcgaa tctcaaaatt tcttccgcgg cgaaggatcg gtttcccagc
     2401 aggaggtcat tcccgcctac cggcagtttc tggggcagaa catctggctt cagcgaaaaa
     2461 tcaaggtggt gatcgacagc ggcaacggaa cggcgggcct ggtggccccg ggaatattaa
     2521 gagacctggg ctgcgaggtg atcgatctct ataccgaggt cgacggccgt tttcccaacc
     2581 atcatcccga ccccaccatc ccggaataca tgcaagccct gatcgccaaa gtgaaagaaa
     2641 ccggggcgga agtcggaatc gggtacgacg gcgacgccga tcggatcggc gccgt
//
LOCUS       DASPRR010000615         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2088172, whole genome shotgun sequence.
ACCESSION   DASPRR010000615 DASPRR010000000
VERSION     DASPRR010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088172"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VEL68_22610"
     CDS             complement(<1..1601)
                     /locus_tag="VEL68_22610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXZ38838.1"
                     /translation="MSDLVLARNPRNPLRLVIRDPVLLATLVILWVLLILFILFPMIR
                     LFLQTFEEEGRLTLANVLKILGDPNQRQALWNSLILATLVGFWGTALGFLFAFTAVRT
                     NLSRGWTFFLDAAIILPLISPPFTTAIAIIFSFGPRGLITYELLGIKNVGVYGLTSTL
                     FSETITFFPIAYLTLKAILAGIDPNVEDMAFSLGSSRWRVFRTVTLPLTVPGLANSFL
                     LLFAASLADFATPLILAGNSFPVLPTQAYLQITGLFDLKGGAVLSFVLLVPAFLVFLL
                     QRFWVSRKYYVTISGKVGAQTQIKSVVPRARILLLALCLFISAFILYFYVLLFYASIV
                     RAFGANYQVTWAHYHVVFTEGMTAIRDTLIIAGAGMPLGGIYAIVLGYLVAKKIFPSR
                     QTMEMVSMINYSLPGTIVGIAYLLAFNDPPVVLTGTAIIIIACYVFRYGPTGIRTTVA
                     ILQQIDPSIEEASFSLGAKSGTTFRRITLPLILPAFFAGLGVVFIRSMTAISATIFLI
                     SINWTLITVRILEKMTELELGVAAA"
     gene            complement(1865..2890)
                     /locus_tag="VEL68_22615"
     CDS             complement(1865..2890)
                     /locus_tag="VEL68_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38839.1"
                     /translation="MRRKGFWTFFGGVIGILLCWYSVAPAAETLNAYSIWPENWARPM
                     FQEFEKATGIKVNFIRFSSGEALARIIAEKNNPRVDVLFGGPVETHIAGIKEGVFEPY
                     KSPSFNALPDRFKDPQGTWLGIADDPLVFMSNNKFLKENKLTPPASWDDLLHPAYKNM
                     LQMADARTSGTAVTRIFSILQVFGRDEAKAFDYMKKLRRNVQVYTKSGGGGTIPVGLG
                     QAGAGIFFIVDALKTRQEGYDVAISFPKEGIGTSVEAIALLKGAKNPETGKKLIDWAT
                     GPGMQSFYAKYKINFIPAHPEVKIEASLAEVIKGANIFPIDENWAGQNRKRIVDRWIN
                     EVLPQQQ"
BASE COUNT          686 a          747 c          894 g          587 t
ORIGIN      
        1 aaggcggcgg ccacgcccag ctccagttcg gtcatctttt ccaggatgcg cacggtgatg
       61 agcgtccagt tgatggatat gagaaagatc gtcgcgctga tggcggtcat ggagcggatg
      121 aagacgaccc ccaggccagc aaaaaatgcg ggcaggatca aggggagagt gatgcgccgg
      181 aaggtcgtgc cggacttggc ccccaggctg aaggaggctt cttcgatgct ggggtcgatc
      241 tgctggagaa tcgccaccgt ggtacggatg cccgtggggc cgtaccggaa tacatagcac
      301 gcaataatga tgatcgcggt tccggtgagc acgacgggcg gatcattgaa ggccaggaga
      361 taggcgatcc ccacgatggt cccggggagg gagtagttaa tcatggagac catttccatg
      421 gtttgacggg agggaaatat cttcttggcc accaggtatc ccaggacgat ggcgtaaatc
      481 cctcccagag gcatgcccgc cccggcgatg atcagggtgt cgcggatggc ggtcatccct
      541 tcggtgaaga ccacgtgata atgagcccag gtgacttgat agttggcgcc gaaggcccgc
      601 acgatggaag cataaaaaag gaggacgtaa aaatagagga tgaaggccga gataaagaga
      661 cacagggcaa gaagcaaaat ccgggccctt ggcacaacgc tcttaatttg ggtttgagcc
      721 ccgactttcc cggaaatggt tacgtagtat ttccggctga cccagaagcg ctggaggagg
      781 aagacgagga acgccggcac gaggagaaca aaggaaagaa ccgctccccc tttcaggtcg
      841 aagaggccgg tgatttgaag gtaggcctgg gtgggaagaa cgggaaagct gttgccggcg
      901 aggattaaag gggtcgcaaa gtcggccagg gaagccgcga agagaaggag aaaagagttg
      961 gccaacccgg ggacggtgag tgggagggtc actgtccgga atacccgcca ccgggagctt
     1021 cctaggctga aagccatatc ctccacattc gggtcaatcc cggccaggat ggcctttagg
     1081 gtgaggtagg cgatggggaa aaaggttatc gtttcagaga agagggtaga ggtcagcccg
     1141 tagaccccga cgtttttgat tcccagaagc tcataggtga tcaaacctct gggcccaaag
     1201 gaaaagataa tggcaatggc cgtggtgaag ggaggggaga taaggggcag gatgatggcg
     1261 gcatccagga aaaacgtcca gccccgggag aggttcgtcc gcaccgcggt aaaggcgaag
     1321 agaaaaccca gggccgtccc ccaaaaacct accagggtgg ccaggatgag actgttccag
     1381 agggcctggc gctggttcgg atcacccagg atttttagga cattcgccaa ggtgagacgg
     1441 ccttcctctt caaaggtctg gagaaagagc cggatcatgg gaaaaagaat gaaaagaatc
     1501 agcaggaccc agaggatgac gagggtggcc aggagaaccg ggtcgcggat gaccaggcga
     1561 aggggattcc tgggattacg agccagaacc aaatcggaca tagtttgctt gaaactcaaa
     1621 actctatcgc acaatgtatc tttctccccc ggagacttcg tcgcaatccg tcatgcccgc
     1681 ggaggcgagc atccaggctt tatgtatttc ctggattctc gctttcgcgg aaatgacgac
     1741 tcttttgtct aattgccgca aaacccccgg acggatgaga gaaagtctga gcgaagcggc
     1801 tggttattaa gaaaatgggg atgcacgggt atgggctccc cttttgggcc gaatcacgcc
     1861 tcgattactg ctgctgcgga agaacctcgt taatccagcg gtcgacgatc cgtttccggt
     1921 tctgccccgc ccagttttca tcgatgggga agatgttggc ccctttaata acctcagcca
     1981 gactggcttc gatcttgacc tccggatggg cggggatgaa attgatcttg tacttggcat
     2041 agaagctctg catgcctggg ccggtggccc agtcgatcag cttcttcccc gtctctgggt
     2101 tcttcgcccc tttgaggagg gcaatcgctt ccacggacgt tccgatcccc tccttgggga
     2161 aggagatcgc cacgtcatac ccttcctgcc tggttttcag ggcgtccacg atgaaaaaaa
     2221 tccccgcgcc cgcctgtccc aggccgacgg gaatggttcc gcctccgccg ctcttggtgt
     2281 acacctggac attgcgacgg agcttcttca tataatcgaa ggccttggcc tcatcccgcc
     2341 cgaacacctg gaggatggaa aagatccggg tcacggccgt gcctgaggtg cgggcatcgg
     2401 ccatctggag catatttttg taggccggat gaagcaggtc atcccaggac gcgggagggg
     2461 ttaatttatt ttccttcagg aatttgttgt tgctcatgaa gaccagtggg tcatcggcga
     2521 ttcccaacca ggtcccctgg gggtctttaa accggtccgg gagggcgttg aaactgggag
     2581 atttgtaagg ttcaaagact ccctctttga tcccggcgat gtgggtttcc acgggcccgc
     2641 caaagaggac atccacccgg ggattgttct tttcggcgat gatcctcgcc agggcttccc
     2701 cggaggagaa acggatgaag tttaccttaa tccctgttgc tttctcaaat tcctggaaca
     2761 tgggccgggc ccagttctcc ggccagatgg agtaagcatt caacgtttcc gcggccggcg
     2821 ccaccgagta ccagcaaaga aggattccga tgactccccc aaagaatgtc caaaatcctt
     2881 tccttctcat tttcatccct ccaccttaaa gttg
//
LOCUS       DASPRR010000616         7418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_467175, whole genome shotgun sequence.
ACCESSION   DASPRR010000616 DASPRR010000000
VERSION     DASPRR010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467175"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VEL68_22620"
     CDS             complement(<1..517)
                     /locus_tag="VEL68_22620"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXZ38840.1"
                     /translation="MDNLISFFLGPVDPLVFLGLIIVTFSGAFITSGFGVGGGMLMTP
                     MVLLLLPPKFGIGLIGPMMLLMSGTGVRQYWKQWDNHCLLVILPAMLAGVWVGTHLLS
                     VISADTVKKIVGALAISFGVLQYLAIDRPKWKERLRPTAPQGVGLGFASGIISAMAHV
                     GGLIFSFYLLPH"
     gene            complement(593..1705)
                     /locus_tag="VEL68_22625"
     CDS             complement(593..1705)
                     /locus_tag="VEL68_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HXZ38841.1"
                     /translation="MKSKVVAQQELENRILHGLACEWERAIWILDSAHRKKMRRPLFS
                     LRDLNGRWGTWSGERREISLSRRLVLNYSWASVREVLFHEMAHQMAEEVLGGQGETAH
                     GPFFRRACDLLRANPRASENYRPLDDRISQEPLDGEDRILARVKKLLALAESSNRHEA
                     EAAMAKAHELISKYNLDLLRTEKQKEFISLLVGRPALRHFLEDYALAGLLQDFYFVQG
                     VWVPGYVPEKGKMGRVLEISGTIPNVRIADYVHNYVQRFIALEWKEYNSGRALNRYRL
                     TDFAQGIIEGFRSKLTSKEQDGGKERTSYALIKKEDPLLKEYLAYRYPHMAMIKTGRG
                     NQDPGVRRDGKRIGKELVISKGVMEGAGNRGRLLPS"
     gene            complement(1841..2335)
                     /locus_tag="VEL68_22630"
     CDS             complement(1841..2335)
                     /locus_tag="VEL68_22630"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXZ38842.1"
                     /translation="MKEMKDLEGIQKFMNRIEEGAFLVVKGNERANVMTIGWALIGVV
                     WRRPVMMVAVRTSRYTYRLIEKADSFTVSVPSGDMKKEINICGSKSGRDLDKFKECGF
                     SALKPKKVSSPVLKIPGYHFECRIINKAPTDPKFLSPDLEDIYPAKDYHTLYFGEILA
                     SYQT"
     gene            3278..4723
                     /locus_tag="VEL68_22635"
     CDS             3278..4723
                     /locus_tag="VEL68_22635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXZ38843.1"
                     /translation="MRFTIFSRLIYGYLVIFLLILAVSVYMIYQLAQFEDITHSILEV
                     DHRMEDLGKKLTDSLLSQMRFEKKFMVVRDEDLYQQFQLARDDFQKYLKEVIYFADTD
                     FQKELLSGVGSRHNTYQALVAEEAAHVRAKEPYSPSGFIMEKDKTADRILENLQTLAI
                     HSQQNTLEKIKKLEQTRINARQLTMGITAIALLAVVILSFFTTMSITYPIAMLIGKTR
                     DIARGVFDGHLPRSAPPEIKELGLAFGSMHERLKTLDQMKSDFFSTISHELRTPLTSI
                     KEGTSLLLEGVGGEISDKQKKLLQIISGESNRLIDLVNSSLDLSKMEAGMMVFNFTQA
                     DISPLIQKVIGEIEPLAMAKKIRLQVGNHERLPLVKMDREKILQVLRNFLGNAVKFSF
                     EGGEIKISACRENGKMKVSVSDTGPGIPKANLATIFEKFQQGPRENSASGKGTGLGLA
                     IAKQIIAAHGGEIWAESEPGQGSSFIFVLPG"
     gene            4666..5187
                     /locus_tag="VEL68_22640"
     CDS             4666..5187
                     /locus_tag="VEL68_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38844.1"
                     /translation="MGGERTRAGKFVYFCAAGLIFFLLSGCMTFKKEFNLSTSQEVTP
                     EKVSQHEEGATSACERLLRSKKLLHQGDYAGSLKECQRVLSLPGKNPPKDQALFQMGL
                     IYADINNPQRDFGKALDFFKRAVKDYPKSPLADEARVWAGVLQENEKLSQVIEKSKQV
                     DIAVEEKKREKAR"
     gene            5194..6561
                     /locus_tag="VEL68_22645"
     CDS             5194..6561
                     /locus_tag="VEL68_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ38845.1"
                     /translation="MSSGNILVVDDDPALLELLRMRLESAQYRVATAQKEEEAIAAVK
                     GQTFDLSVVDLKLGRGDGISLMEEIHSLSPEMPIIILTAHGTIESAVEAMRKGAYSYL
                     TKPFEPRDLFFQIEKALENRRLTSEVQRLQELLKEKYDFAHIVAKSEKMRAVLEMVSR
                     IAKIDSTVCIRGESGTGKELIARAIHLASDRKNKHFVAINCAALPEPLLESQLFGHEK
                     GAFTGAVRSTKGLFTQAHEGTIFLDEIGDMPLSIQAKLLRVLQERQFYPLGSEKPVEV
                     DVRVIVATQKDLTEQVKQGLFREDLFYRIHVIPIHLPPLRERKEDIPPLVEHFLKKFS
                     QQMKKEVKGLAPGVLKGLMLQEWPGNVRELENTIEYAVAMTQLDFITEDFILQAKTAE
                     PSGSPKPFKEAKDSFEKEYLSHLLELCQGNVSQAAKMAGKYRADFYDLLKKHNLQTAD
                     FKKSG"
     gene            6804..7208
                     /locus_tag="VEL68_22650"
     CDS             6804..7208
                     /locus_tag="VEL68_22650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ38846.1"
                     /translation="MVVDDNLPLASLLQMMLEEEGYEVQFAGNGLEAYANYLLFRPEV
                     VITDLHMPKANGMELMNNIRKQDPGVKTIYMSADMRPFFPLLDEEKSRYPVSLLPKPF
                     SKEELLNLLAKFMDGGEGGVLLDSPEPRRMEG"
BASE COUNT         1831 a         1971 c         1987 g         1629 t
ORIGIN      
        1 tgtggggaag caggtagaag gaaaaaataa ggccccccac gtgagccatg gcgctgataa
       61 tgcccgaggc gaaccccagc cctactccct gcggggcggt cgggcggaga cgctctttcc
      121 atttcggccg gtcgatggcc aggtactgga gaactccgaa agaaatggcc agggccccca
      181 cgatcttctt taccgtatct gcggaaatga ccgaaagcag atgagtgccc acccagacgc
      241 cggccaacat cgccgggaga ataaccagca agcaatgatt gtcccactgt ttccagtact
      301 gccgcactcc ggtgccggac atcaggagca tcatgggacc gatcaggccg attccaaact
      361 tgggagggag gagaaggagg accatggggg tcatcagcat gcccccgccc accccgaatc
      421 cagaggtgat gaaggcgccg ctgaaggtga cgattatcag gccgaggaaa accaaggggt
      481 ccacagggcc gagaaagaag gaaatcaggt tatccatttc aattccaatt ggccgcagag
      541 gaaaaactga tttgttgggg cggtttgcga accgtcccta agggaacaaa cctcaagagg
      601 gaagcaggcg gccgcggttc cccgctccct ccataacgcc tttggatatg accagctcct
      661 ttccgattcg ctttccatcc cttcggaccc ccgggtcctg atttcccctc ccggttttga
      721 tcatagccat atgggggtag cgataagcca agtattcctt caagagcggg tcttccttct
      781 ttatgagggc gtaggaggtc cgttccttcc ccccatcttg ctctttcgac gtaagtttcg
      841 aacggaaccc ttcaattatc ccctgcgcga aatctgtcaa tcggtagcgg ttcagagccc
      901 tgccggaatt gtattccttc cactccaggg cgataaaccg ctgcacgtag ttgtggacat
      961 aatcggcaat ccgcacattg gggatggtcc cgctgatttc caggacccgg cccatcttcc
     1021 ccttctcggg aacatacccg ggcacccaga ccccctggac aaaataaaaa tcctggagga
     1081 ggccggccaa ggcataatcc tcaaggaaat ggcgcagagc cggcctcccc acgaggaggc
     1141 tgataaattc tttctgcttt tcagtccgta gaaggtccag gttgtacttg gatatcagtt
     1201 cgtgggcctt ggccattgcg gcttcggcct cgtgccggtt ggaactctcg gccaaggcca
     1261 gaagcttttt gactctggcc aggatccggt cttctccgtc caggggttct tgggaaatgc
     1321 ggtcgtccag gggtcgatag ttttcggatg ccctggggtt ggcgcgaagc aggtcgcagg
     1381 ccctccggaa aaaaggtcca tgggcggtct ccccttgacc tcccaggact tcttccgcca
     1441 tctgatgggc catttcgtgg aaaaggactt cccggactga agcccaggaa tagtttaaga
     1501 ccaacctccg gctcagggaa atctcccgcc tttccccgga ccaagttccc catcggccat
     1561 tgaggtcccg caaggagaaa agggggcggc gcatcttctt tcgatgggcg gagtccagaa
     1621 tccagatggc cctttcccat tcgcaggcca ggccgtggag gatgcggttt tccagctctt
     1681 gctgagcaac caccttgctc ttcatttgac cgtctccatc tgccggaaca caaagacccg
     1741 gcgttcctcg tttgatcggc ccagacgcca gtaaccaacg atcaacaatc ggaaaggagg
     1801 tacgagcgac cggcggcaag caacgggggg ccggcagcgg ttatgtctgg taagacgcca
     1861 ggatctcccc aaaataaagg gtatggtaat ccttggccgg atagatatcc tccaggtccg
     1921 gggagaggaa tttcgggtct gtgggggctt tgttgataat ccggcactcg aaatggtatc
     1981 cagggatctt caggacgggg ctactgactt ttttgggttt taaagcggaa aaaccgcact
     2041 ccttgaattt gtccaagtcc cggccggact tggagccgca gatgttgatt tcctttttca
     2101 tatcccccga gggaacacta accgtgaagc tatccgcctt ttcgatcaat cgatacgtat
     2161 agcgggacgt cctcacagcc accatcataa ccggccgtcg ccaaacaaca ccgatcagcg
     2221 cccagccgat ggtcatcacg ttggctctct catttccctt gaccacgaga aaagcccctt
     2281 cttcgatccg gttcatgaat ttctgaattc cttctaaatc tttcatctct ttcatatccc
     2341 tctcctcaaa atggaaattc aaaaacccat gctaacacga agagggaaat tggaaaagtc
     2401 ccaaccctcc ctcagcccgc cgtcgcatat ccgaaaccgg ccaacccgga accaaataaa
     2461 agaatgtaca gccggccctc ccgtcttcgg ggggaatgcc cgttaaaagg gtaaccagca
     2521 gtcgcttcgc gtgacggccg ttgcaattta ctaccctctt gagcacaact ctattctcaa
     2581 ggggtcttct cgcgaacgag tgggtgattt cggtctattt tcaatcggcc actatgctcg
     2641 cccatgtggc caaggttatt cggccaggcc cacagggtgc gtcctgccca tggcaagaca
     2701 gaatgaggca tgagttcttt catcggttcg ggtctgaaaa ttctttggtt gcccgggccc
     2761 gagtcagggg tcatttttcc gaaagggtgt cggggaccgg tgctttgcgg aacccaagca
     2821 accgctacgg aatccgggta tttcctgccg aatcgatcac gccatgggcg gaacgcaccc
     2881 cctggtctga gaatatttct gttccctttc tgcccactca aatgggagag gagcatatct
     2941 aaggggatag agaccgggaa tgaaaatcat cttgacttta ttgataaagt attgaattct
     3001 attcttagcg gttgctaata aataatgggt tccctcgaaa tctattgccc cattctgaag
     3061 gcaaacggta ccctcagcct gggagagagg gggagcagag ggattatttt tgcctatccc
     3121 cccggggggt caaagtatta tgaattgggg aatctccgtt tcattatctt ccttggattt
     3181 ctcggcgttt atcccacctt cgaacacctg gtcaaatccc gcgggctatc cctcccatga
     3241 aagttagcct gcggtttagg ggatggaatt tcctgggatg agattcacaa tattttcccg
     3301 gttaatttac ggttacctgg tgatcttctt gctcatattg gccgtgagcg tctacatgat
     3361 ttaccagttg gcccaattcg aagacatcac ccattcaatt ctagaagtgg atcaccggat
     3421 ggaggatttg ggaaagaaac tcactgattc cctcttgtcc caaatgcggt ttgaaaaaaa
     3481 attcatggtc gtccgggatg aagaccttta ccaacaattc caactggccc gggatgattt
     3541 ccagaaatat ctgaaagaag ttatatactt cgccgatacc gatttccaga aagagttgtt
     3601 aagcggcgta gggtcccgcc ataatactta ccaagccttg gtcgcggaag aagcagcaca
     3661 tgtgcgggcc aaggagccct attccccttc cgggtttatt atggaaaaag ataagacggc
     3721 ggatcggatc ctcgaaaatt tgcagaccct ggccatccat tctcaacaaa ataccctgga
     3781 aaaaataaaa aagctggaac aaaccagaat caatgcccgc cagctgacga tgggaattac
     3841 cgccatagct ctcctggcgg ttgtcatcct gtcctttttt accaccatga gcattaccta
     3901 ccccattgcc atgctgatcg ggaagacccg tgacatcgcc cggggggtct tcgatgggca
     3961 tttgcctcgc tcggccccgc cagaaataaa ggaattgggc ctggcctttg gttccatgca
     4021 cgagcggctg aagacccttg atcaaatgaa gtccgatttc ttctccacca tatcccatga
     4081 gctgcgcacc cccctgactt ccatcaagga ggggaccagc ctcttgctgg aaggggtggg
     4141 cggggagatt tctgataaac agaagaagct cctgcaaatt atctccgggg agagcaaccg
     4201 gctgattgac ctggtcaatt cctccctgga tttatccaaa atggaagcgg gaatgatggt
     4261 ttttaatttt acccaggcgg atatcagccc gttgattcaa aaggtgatcg gggagattga
     4321 acctctggcc atggccaaga aaattcgcct ccaggttgga aaccacgaac gtctgccgct
     4381 ggtgaaaatg gaccgtgaaa aaattctcca agtcctgcgg aatttcctgg ggaacgcggt
     4441 aaaattttct tttgagggcg gagaaatcaa aatttccgcc tgccgggaaa acggtaaaat
     4501 gaaagtttcg gtaagcgata ccgggccggg catacccaag gccaacctgg cgaccatctt
     4561 tgagaaattt cagcaaggac cccgggagaa ttctgcatcc gggaaaggga cgggattggg
     4621 gttagctata gccaagcaga tcatcgctgc acacgggggt gaaatatggg cggagagcga
     4681 acccgggcag ggaagttcgt ttatttttgt gctgccgggt taatcttttt tctcctttcg
     4741 ggttgcatga ccttcaagaa ggaatttaat ctgagcacaa gccaagaagt tacgccggag
     4801 aaagtctccc agcatgagga aggtgcgact tcggcctgcg aacgcctcct gcgctccaaa
     4861 aagctcttgc accaggggga ttacgccggt tctctgaagg aatgccagag agtcttgtcc
     4921 cttccgggga agaaccctcc caaggaccag gctcttttcc agatggggct gatctatgcc
     4981 gacataaaca atcctcaaag ggacttcggc aaggctctgg attttttcaa aagggcggtc
     5041 aaggattacc ccaagagccc cctggccgac gaggccaggg tatgggcggg ggtgcttcag
     5101 gagaatgaga aattgagtca ggtgattgag aaatcgaaac aggtggatat cgccgtggaa
     5161 gaaaaaaaga gagagaaagc gagatagaag ggcatgtcgt cgggaaatat cttggtggtg
     5221 gatgatgacc cggcccttct ggagttgctc cgcatgaggc tggagtcggc ccaataccgg
     5281 gtagcgaccg cgcagaagga ggaagaagcg attgccgcgg tcaaagggca gacctttgac
     5341 ctgtcggttg ttgacctgaa gcttggccgg ggggatggaa tttcgctgat ggaggagatt
     5401 cattccctga gtccggagat gcccatcatc atcctgaccg cgcacggcac cattgaaagc
     5461 gcggtggagg ccatgcgcaa aggagcctac agctacctca ccaagccttt cgaaccccgg
     5521 gacctcttct tccagatcga aaaagcattg gagaaccgac ggctcacctc ggaggttcaa
     5581 aggctgcagg agctcttgaa agaaaaatat gattttgccc atatcgtggc caagagcgaa
     5641 aagatgaggg ccgtgctgga gatggtgtcc cggatcgcga aaatcgattc aaccgtttgc
     5701 attcgcggag aaagcggcac gggaaaagaa ctgatcgccc gggcgattca tctggcgagt
     5761 gaccggaaga acaaacactt tgtggccatc aactgtgcgg cccttccgga gccgctgttg
     5821 gaaagccagc tcttcggcca tgagaaggga gccttcaccg gggccgttcg aagcactaaa
     5881 gggttgttca cccaggctca tgaaggcacg attttcctgg atgagatcgg ggatatgcct
     5941 ctttcgatcc aggccaagct tctgcgggtc ctgcaggagc gccaatttta ccccctgggc
     6001 agtgaaaaac cagtggaggt tgatgtccgg gtgatcgtgg ccacccaaaa ggacctaacc
     6061 gaacaggtga aacaggggct tttccgggaa gacctttttt atcgaatcca tgtcatcccc
     6121 atccatcttc ctcccctgcg ggagaggaaa gaagacatcc ctcccctggt ggaacatttt
     6181 ttgaaaaaat tcagccagca gatgaaaaaa gaggtaaaag gcctggcccc cggggtcctg
     6241 aaggggctca tgctccagga atggccgggg aatgtgcggg agctagaaaa taccatcgag
     6301 tatgccgtgg caatgaccca gctggatttc atcaccgaag actttatcct ccaggccaag
     6361 actgctgaac cgtcagggtc cccgaagccc tttaaagaag ccaaggactc ttttgaaaag
     6421 gaatacctat cccatctcct ggaactctgc cagggcaatg tgagccaggc cgccaaaatg
     6481 gccgggaaat accgcgccga tttctatgac ctcctgaaaa aacataattt gcaaacagct
     6541 gactttaaga aatccgggta gggtgtagga aatctactcc cagggtaaac gagggaaata
     6601 aagggaattc aaccccgaac gccttctctc cgctcgtatc cgtatggaaa accctcctca
     6661 tccctctccc tagctcaacc gctcctcaag gaaaccccat taaaatcaat accctttctt
     6721 ctctgcgagc tttggcacat cctttgctcg ggaagcagcc aagccaaaat ctgagccggg
     6781 aaggaagaag aaatacgaaa gtattggttg tcgacgacaa tctcccgctg gcttctctcc
     6841 tccagatgat gctggaggaa gagggctatg aggtccaatt cgcggggaac gggcttgagg
     6901 cctatgccaa ctatctccta ttcaggccgg aggtggtgat caccgacctc cacatgccca
     6961 aagccaacgg aatggagttg atgaacaata tccgcaagca agaccccggg gtgaagacca
     7021 tatatatgag cgcagatatg cgtccctttt tccctcttct ggatgaagag aagagcaggt
     7081 atccggtaag ccttctgccc aagcccttct ccaaggagga attgctgaac ctgctggcga
     7141 agttcatgga cggcggagag ggtggagttt tgcttgactc tcccgaaccg cgaaggatgg
     7201 aaggatagaa aaaaaggagg aaaaaagatg ccggagggga atcatcgaaa aattggttct
     7261 attgtccctg accctcctcc ttctcaaggc caaggggggg ccccgccatt cagtgttggg
     7321 aaatccgaat tccctccgtc ttttccgcca aggccgcaaa acaggaatca gccccgggaa
     7381 gtaagccgta tcctcagtcc tcaggggtat ccagaatc
//
LOCUS       DASPRR010000617         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_963900, whole genome shotgun sequence.
ACCESSION   DASPRR010000617 DASPRR010000000
VERSION     DASPRR010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963900"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /gene="yddG"
                     /locus_tag="VEL68_22655"
     CDS             complement(<1..956)
                     /gene="yddG"
                     /locus_tag="VEL68_22655"
                     /inference="COORDINATES: protein motif:HMM:NF008676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid DMT transporter YddG"
                     /protein_id="HXZ38847.1"
                     /translation="MRKVHLDRNTVAGLGAILIWGTSIALARSITEQLGPLTGAAAIH
                     LVGGAFCWGQFFYRFRGTVLSFRQLPGKFLLGCGALFVLYMLALYLAVGLASNRSQVL
                     EIGLINYLWPALTILFSLFLLNKKAKLALWPGTFLALLGVFLVLTQGHSISFSSLLAN
                     VSGNPPAYTLALAAAVSWGLYSNLTRRWAAPRMEGGVELFVTVTGVVFLILRLLHPEG
                     GSWTLRAGLEVTFLGMATALGYLLWDMAMRKGDVVLVAAFSYFTPLFSTLLSCLYLQV
                     TAGVSLWLGCLLIVAGSLLSWVAVSDRKTHPLPLNQKEFESR"
     gene            complement(1039..2016)
                     /locus_tag="VEL68_22660"
     CDS             complement(1039..2016)
                     /locus_tag="VEL68_22660"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ38848.1"
                     /translation="MRLNSLRSRAVPPLLVLMLILFLSPGGWAQQYPTKPINMLIGWG
                     AGGSTDVTLRALSDAAGKILGQPIVAQNKPGGGSAVALALLKNEKPDGYTVGNLSSAG
                     IVGQHLRKVPYDSTQDFTPIIRYGDYIHGITVKADAPWKNIKELVAYAKANPGKLKYS
                     SPGTGSSHHLVVEALGIKEGIKWTHIPYKGGHEATTALLGGHVDFQTCASEWKPYVDS
                     GALKLLATTGEKRYEKYSDVPTLIDQGYKIWAITLVGILGPKGIPTPIVEKLHGGFKQ
                     AMENPEFKKAMSNFDMPIVYRDPQGLLKDTKEISDMWGPIIKQLGIKQE"
     gene            2702..3985
                     /locus_tag="VEL68_22665"
     CDS             2702..3985
                     /locus_tag="VEL68_22665"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXZ38849.1"
                     /translation="MSERLPSVHKEEGRTFAAQPSGYLLHKASLYFFGGILFLAAWKG
                     QAGIVILIGLVFSAAGLAALWSRVSLVGVRCRGRVRERRLFPGESTELTLEIFNRKPL
                     PMPWLRVDDEIPVGLAQDLAPVPASRPGYFLLSRWASVLWYRKVSWNHRLHGEKRGYY
                     RLGSLTVTSGDIFGFYPRWRQFASADPVIVYPRIFPIERLEVPSQHPMGEARSLRRIF
                     QDPTRTIGLRDYRPQDSLKHIHWKASARHQELQVKVFEPTTTLKVSLFLAGDGYPPDS
                     PLSEEEFELAVSTAASIAHHLINQGTPVGLICNGCQADSGQAIQIPPGGSRDQLMEIL
                     EALAKVTCRAKDSSEAFLERESKSLPSGTTLILVASRTPESFFRLLRDLKERGTKLLL
                     LRIGDPKETTVDRMIPSHLIRHPGDLLTINPEVQA"
     gene            3982..5376
                     /locus_tag="VEL68_22670"
     CDS             3982..5376
                     /locus_tag="VEL68_22670"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HXZ38850.1"
                     /translation="MNRGWIAITLYAAVAGLEVSWFWAFLDLLNQKGTNGSLPLLFLI
                     GFYPLAFVFHRMLRGLSWMKALPLGCLAWAALVLLLGKVHFFAHTGWEDSQWLRDFGY
                     DLARAVHGLTPESLVALSSAVFWGFGCRLASVHIQFATLVGEFQFGLAILLTLFLLSA
                     QWELSTATLIPLTLAFFFFSLSGIAVSHASERESWVSGHYGHRWFAFLLLTIGFILGV
                     GWLISAVANPSLIESILSILATAGQWIWAWIVKFFIFLGELIPPPKPAELPPAARLSQ
                     MKGPAETPFILFSDSVREVIRFIWGAGVISLLLIALWRISSQVFEWFRQKMGGMGDAE
                     VEPLPGAFQEDLLNFLKMLLRIFGVPWPFRRGSRAERLLPGAELVRKIYRQLSAWAAK
                     KGHARNPAQTPYEFLQGLTLWLPERRGDLTSITDQYVRTRYGLSAPTESGLEELRQRW
                     QRVRQTRFKKRPKK"
     gene            5521..>6401
                     /locus_tag="VEL68_22675"
     CDS             5521..>6401
                     /locus_tag="VEL68_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXZ38851.1"
                     /translation="MNQSVAPPAAEVGRIKEAADRLIQNVNRVIVGKKETIQLVFIAL
                     LCEGHVLIEDVPGVGKTLLAKAMARSAGCRFKRIQCTPDLLPSDVTGIHYFNQKASAF
                     EFRPGPILANIVLVDEINRAMPRTQSSLLECMQEQQVTVDLETVPLPRPFLILATQNP
                     IELEGTFPLPEAQLDRFLMRVRLGYPEDGEEEAILTRFRESNPMEGLSSVLDASAVLD
                     LQKRCRQVYVEDSVRRYMTDLTRATRNHEKIRLGASPRATLGLYLASQAKAASQGRDF
                     VIPDDVKSLAVPVLSHR"
BASE COUNT         1321 a         1851 c         1867 g         1362 t
ORIGIN      
        1 tgccgggatt caaattcttt ctggttcaat ggcagggggt gcgtcttgcg gtcggagact
       61 gccacccagc tgagaagaga accggcgaca atcagcagac accccagcca caggctgacc
      121 ccggctgtga cctggaggta aagacagcta agaagggtgg aaaaaagagg agtgaaatag
      181 gaaaaggcgg cgacaaggac cacgtcgccc ttacgcatgg ccatgtccca gagaagatag
      241 ccgagggccg tggccattcc caagaaggtg acttccagac ccgcccgcaa ggtccatgat
      301 cctccctcgg gatggaggag acgaaggatc aggaaaacga cgccggtgac agtgacaaaa
      361 agttccactc cgccttccat ccgaggggcg gcccagcgcc gggtcaggtt ggagtagagg
      421 ccccaggaaa ctgcggcagc caaggccagg gtataggccg gcggattgcc ggatacattg
      481 gcaagcaggg aggaaaagga aatcgagtgg ccctgggtaa gaacaaggaa aactcccaag
      541 agggccagga aggtccccgg ccacaaagcc agcttggctt tcttgttgag caagaacaag
      601 gagaacaaaa tggtgagggc cggccagagg taattgatca ggccgatctc caacacctgg
      661 ctccggttgc tcgcaaggcc cactgccagg tagagagcca gcatgtaaag gacgaagagg
      721 gctccgcagc cgagtaagaa tttccccgga agttggcgga aagaaaggac cgttccccgg
      781 aagcggtaga agaactggcc ccagcaaaag gcgcccccca ccagatggat ggctgcggct
      841 ccggtcaggg gcccaagctg ttcggtgata gaccgagcca gggcgatgga ggtgccccag
      901 atgagtattg ctcccagccc ggcaacagta tttcgatcta gatggacttt tctcaccccc
      961 tatttctttc ctcaaaccca gtcttagtgc ctcgaacctc gaacccgctc ttggtgcctc
     1021 aaacctctaa cctcaatcct attcctgctt gatccccagt tgcttgatga tcggccccca
     1081 catatcactg atttccttgg tatccttcag aagcccttgc gggtcccggt agacaatggg
     1141 catgtcgaaa ttactcatgg cctttttgaa ctccgggttt tccatggcct gtttgaagcc
     1201 cccgtggagc ttctccacga tgggagtcgg tattcccttg gggccgagga ttccgaccag
     1261 ggtaatagcc cagatcttgt acccctggtc gatcagggtg gggacatccg aatatttctc
     1321 gtaccgcttc tccccggtcg tggccagcag cttcagggcg ccgctgtcca cgtaaggctt
     1381 ccattcggaa gcgcaggtct ggaagtccac atgccctccc aggagggctg ttgtggcttc
     1441 atgcccccct ttgtaaggaa tgtgggtcca cttgattccc tccttgatcc ccaaagcctc
     1501 caccaccagg tggtgagaag accccgttcc cgggctgctg tatttcagct tcccgggatt
     1561 ggctttggca taggcaacca gttccttgat gttcttccat ggagcgtcgg ccttaaccgt
     1621 gattccatgg atgtagtcac cgtagcgaat aatgggggtg aagtcctggg tcgaatcgta
     1681 gggcactttt cgcaaatgct ggccgacaat tcctgcggat gaaagattcc ccacagtgta
     1741 gccgtcaggt ttttcattct tcaggagagc gagggctacg gccgacccgc ctccaggctt
     1801 gttttgggcc acgatgggct ggccaaggat tttgccggct gcgtcggata gcgcccgcag
     1861 ggtcacgtcc gtggagcccc ccgctcccca cccgatgagc atgttgatcg gctttgtcgg
     1921 gtactgctgg gcccacccgc cgggcgatag gaaaagaatc agcataagaa caagcagggg
     1981 cggcaccgcc cggctccgga gagaattcag ccgcatgatg cgatctcctt ttctctcaaa
     2041 aattcgggtc ttcccccaat cgagtggatg gaaaggcaac agaatttatc tcagccaagg
     2101 gggtgcgttc cgttcatggc gggacagaat cggcgggaaa aacccggatt ccggagcgat
     2161 cgcttgggtt ccgcaatgca cctgtcctcg actcccattt cggaaaaccg aatcttgact
     2221 caggtccgga cagccaaaga attttccgac ccgaaccgat gaaaagactg gcgcctcatt
     2281 ctgtcccgcc atgggcggaa cacgccccct gggcctggca gaatcccccc cgcccgggaa
     2341 gcgaccataa tggccgatta aaaatcgacc gaaatcaccc ccccgattgg gagaaggccc
     2401 agaacatcct attattcaaa atacccggat tcagctaaaa agaaaaggag gatcctggcg
     2461 tctgcgccct actgcccgcc gggtctgaag cggtgaagca ggcaaccgat ttggctggaa
     2521 agaaaaatta cctccatgct atattcaaac agcactttcc gggtagagcc ggcgttaagg
     2581 agggagccag attagggttt cccgcggtcc cgggaggtgc tttatttttc cctgaatgcc
     2641 gataaatggg ggaaaggacc ctcagctccc tgaatcgggt ccgcatttcg agtcattcaa
     2701 aatgtcggaa cgactaccct ccgtccataa ggaagaaggc cgaaccttcg cggcccaacc
     2761 ttccggctac ctgctccata aggccagcct ttactttttt ggaggaatcc ttttcctggc
     2821 cgcctggaaa gggcaggcgg ggatcgtcat ccttatcggc ttggtcttca gcgcggccgg
     2881 cctggctgcc ctgtggagcc gggtgtcgct ggtgggggtc cgctgccggg gccgggtccg
     2941 cgagcggcgc ctttttccgg gggaatccac cgaactcacc ctggaaatct tcaaccgtaa
     3001 gccgctgccc atgccctggc tcagggtaga cgacgaaata cctgtcggac tggcccagga
     3061 ccttgccccg gtcccagcga gccgccccgg ctatttcctg ctgagccgct gggcatcggt
     3121 tctgtggtac cgcaaggtaa gctggaacca ccgactccat ggggaaaaac gagggtatta
     3181 tcgtctcgga tcgctcaccg tcacctcggg agatatcttt ggcttttatc cgcggtggag
     3241 gcagtttgct tccgctgatc cggtcatcgt ctatcctaga attttcccca tcgaacggct
     3301 ggaggtccca tcccagcatc ctatgggaga ggcaaggagc ctccggcgca ttttccagga
     3361 ccctacccgg acgattggcc tgcgggatta tcgtccccag gacagcctca agcacattca
     3421 ctggaaggcc agtgcccgcc accaggagtt gcaggtgaag gtctttgagc ccacgaccac
     3481 cctgaaagtc tccctcttcc tggcgggaga cgggtatccg ccggacagcc ccctgtccga
     3541 agaagagttt gagctggcgg tgagcacggc ggcatcgatc gcccatcacc tcatcaatca
     3601 gggtacccct gtggggctga tttgcaacgg atgccaggcg gactcgggcc aggcgattca
     3661 gattcccccg gggggaagcc gcgatcaact gatggagatc ctggaggctt tagccaaagt
     3721 cacctgccgg gcaaaggatt cttcggaggc ctttctggaa agggaaagca aatccttacc
     3781 gtcgggaacc accctcatcc tcgttgcttc ccgtaccccg gaatcattct tccggctgct
     3841 aagagaccta aaagagagag ggaccaaatt gctgctcctg cggatcgggg acccgaagga
     3901 aaccacggtg gataggatga ttcccagtca cctgatccgc catcccgggg atcttttgac
     3961 gatcaacccg gaggtccagg catgaaccgg gggtggattg cgattacctt gtacgcggcg
     4021 gtagcagggc tggaagtctc ctggttctgg gccttcctgg accttctgaa tcagaaaggt
     4081 acaaacggtt ccttgcccct cctgttttta atcggtttct accccctggc tttcgttttt
     4141 caccgaatgc tgcgcgggct gtcctggatg aaggctctcc ctttgggctg cctggcctgg
     4201 gcggccttgg ttcttcttct ggggaaagtc catttttttg cccatacggg ctgggaagac
     4261 tctcaatggc ttcgggattt cgggtacgac ttggcccggg cagtccatgg cttgaccccg
     4321 gagtcgctcg ttgctctgag cagcgccgtt ttctggggat ttgggtgtcg ccttgcctct
     4381 gtgcatattc aatttgccac cctggtcggc gagttccagt tcggcctggc cattctcttg
     4441 acccttttcc ttctgagcgc ccaatgggaa ttgagcacgg ccaccctgat ccccctgacc
     4501 ctggcctttt ttttcttctc cttaagcggc attgcggttt cccacgcctc ggagcgggaa
     4561 agctgggtct caggccatta cgggcatcgc tggtttgctt tcctgctttt gactatcgga
     4621 tttatcctgg gggtgggttg gctgatcagc gcggtggcga acccttcgct gatcgaatcc
     4681 attctctcca ttctcgccac ggcgggtcag tggatttggg cctggatcgt aaaattcttc
     4741 attttcctgg gagaacttat tcccccgccc aagccggcgg agcttccgcc ggctgcgcgc
     4801 ctgtcccaga tgaaagggcc ggccgagacc ccgttcatcc tcttttccga ctcggtgcgg
     4861 gaggttattc gattcatctg gggggccggg gttatctccc tcctcctgat tgccctgtgg
     4921 aggatttcct cgcaggtctt tgagtggttc cggcaaaaga tgggaggaat gggggacgcc
     4981 gaggtcgagc ccctcccggg ggccttccag gaggaccttc tcaatttctt gaaaatgctg
     5041 ctccggattt tcggagtccc atggcctttt cgaagaggaa gcagagctga gcgccttctg
     5101 ccgggagccg agctggttcg caaaatctac cgccagcttt cggcgtgggc ggcaaagaag
     5161 ggacatgcgc gaaacccggc gcaaacccct tatgaatttc tgcaaggact gaccctgtgg
     5221 cttccggaga ggcgggggga tttgacctcc atcaccgatc agtatgtgcg tacccggtac
     5281 ggcctctcgg cgccgacgga gagtggactg gaggaattgc ggcagcgctg gcagcgggtg
     5341 cggcaaaccc ggttcaagaa gcgtccgaaa aaataataga agacagcaga attgtacgca
     5401 aaatcgtatt aaaattgtaa ggggaaccct gcgtggttgc ctatcctgag tgcggccaca
     5461 tcgggccgcc tctaaaatat tatcttgttt ggttccggct atgcaggtaa ggagataaat
     5521 atgaatcaat cggttgcccc tccggctgcg gaagtgggca ggatcaagga agctgcggat
     5581 aggctaatcc agaacgtcaa ccgagtcatc gtggggaaga aggaaaccat tcagctggtt
     5641 ttcatcgccc ttctctgtga aggacatgtg ctcatcgaag atgtccccgg tgtgggaaag
     5701 accctgctgg ccaaggccat ggcccggtcc gcgggctgcc gtttcaagag gatccagtgt
     5761 acccccgacc tcctgccctc ggacgtgacc gggatccact acttcaacca gaaggcttcg
     5821 gcctttgaat ttcggccggg ccccatcctg gccaacattg tcttggtgga tgaaatcaat
     5881 agggccatgc cccggaccca gtcttcgctc ctggagtgca tgcaggagca gcaggtcacc
     5941 gtggacctgg aaacggtgcc cctgccccgg cctttcctga tcctggccac acagaacccc
     6001 atcgaactgg aagggacctt ccccttgccg gaggcccaac tcgaccgctt tcttatgagg
     6061 gtcagattgg gctacccgga ggatggggag gaggaagcca tcctgacacg gttccgcgag
     6121 tcgaatccga tggaagggct atccagcgtc ttggacgcgt cagcggtcct agacctccaa
     6181 aaaagatgcc ggcaggttta tgtggaggac tctgtgcgga ggtatatgac cgatttgacc
     6241 cgggcgaccc gcaatcatga aaagatccgg ttgggggcca gcccgcgggc gaccttgggg
     6301 ctctaccttg catcccaggc caaggcggcc agccagggaa gagatttcgt gattcccgac
     6361 gacgtgaaat cgctggccgt tccggtcctc tcccatcgct t
//
LOCUS       DASPRR010000618         9068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1769569, whole genome shotgun sequence.
ACCESSION   DASPRR010000618 DASPRR010000000
VERSION     DASPRR010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769569"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VEL68_22680"
     CDS             <1..928
                     /locus_tag="VEL68_22680"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ38852.1"
                     /translation="SAGSVLAQGKYPTKAIDIIVPYAPGGGTDIMYRNIEKIISQYKL
                     VSQPVNIVNKPGGGGSIGKAFCLSRPPDGYSFTCFDLGTVSHQIDGKAKWDYRKDFSY
                     IARIVSDINLIIVRTDSPINNAQDLVETVKKKGPKSVSIGGTAIGGPDQIGHFVLNKA
                     TKQQFNYVPYNSGGEVVTNLLGGHIDAAWANPNECIGQLEAKQVKIVGVCTEKRSPMF
                     PNHPTFREQGYDVISVQTRSMVGRAGIPKEAIDYWVAILDKVRNTPEWKNFLKTSLLD
                     DGWLVKEEFFKDAENDYNMVKPIMDELGLSKK"
     gene            1009..1464
                     /locus_tag="VEL68_22685"
     CDS             1009..1464
                     /locus_tag="VEL68_22685"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ38853.1"
                     /translation="MKNKDFYSGLILLILSGTIFVLILRSPAPPDSSGPGPFFLPFIS
                     ALVIAGLSIALLLQSQKQPETGAAAPQTKRMWNRLIWIVVWCFAYGFSIEKLGYLLST
                     GVVTFALLAYFNRRKWILNLLFSLFTPISIYILFDTLLKVPLPKGWFGF"
     gene            1476..2972
                     /locus_tag="VEL68_22690"
     CDS             1476..2972
                     /locus_tag="VEL68_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ38854.1"
                     /translation="MEILHNLLYGFTVALSPANFLYCFLGVFIGTIVGVLPGLGVMNA
                     VAILIPMTFGMNPTAAMIMLSGIYYGAMYGGSTTAILINTPGEAPAIMTTLDGYQMAL
                     QGRAGPALGIAAFGSFIAGTFATVMLMVLAEPLVKVALSFGPTEYFSMLILALCTLGG
                     LMGESVIKGLLMAAFGIFLGTVGIDPVAGVDRFTFGIPDFMDGIGFLPVAIGLFAIAE
                     VLLTAEVEMMRPVVEKIKGLLPSLKDWMESKWAITRGTVIGFLIGCMPGGGATLASVV
                     SYLVEKKFSKNPERFGKGAIEGVAGPESANNAASGGAMVPLLTLGIPSTGVSAVLLGA
                     LMLYGLRPGPLLFQNSPELVWGLIASLYIGNFLLLLINLPLIGIWVRMLKVPFPLLLP
                     FILVIAITGTYATNNSVTEVYVTLVFGVIGYFMKHADLPAAPVIMGIVLGPLLETHFR
                     RALIISNGSYATFFTHPVSAIFLCAAVVSILYPLIKWGIKRKNVTPEA"
     gene            3252..3875
                     /locus_tag="VEL68_22695"
     CDS             3252..3875
                     /locus_tag="VEL68_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXZ38855.1"
                     /translation="MGLIRLLFTNPFAFLLLIIPLLYSIIIHELAHGWVANKMGDSTA
                     KWLGRLTLNPMRHLDPIGTLMLFFFGFGWAKPVPVNFNNLHDPRKRLDLVSAAGIIAN
                     TLLAFFALLILRVFNPAPFGAISTFLIYMAQINIMLAAFNLIPIPPLDGSKILMGFSS
                     PQFQYTLARLEPYGFFIIIALLYLNILNPLIALFEWIITGIISLLLP"
     gene            3897..5285
                     /locus_tag="VEL68_22700"
     CDS             3897..5285
                     /locus_tag="VEL68_22700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ38856.1"
                     /translation="MAWTFTVLGGGQEIGANSYLLSLSGVPLLLDAGLHPKKYGFESL
                     PDYDGIERELEAIFITHAHLDHVGSLPVINHRHPQAPVFATLPTKDIALRMLHNTVTV
                     MNIFREEKGLMEYPFFDHEDLWPLEGEIMGLDYRIARSSSAPGKQIYSEQLHLRGGIQ
                     ARFLPAGHVLGAGGILFRQGRRFLYYTGDISLQAQVLIPGAVLPDERIDTLILECTYG
                     NDPAYHSRNRHQEVESFAREAASILAAGGCILIPAFALGKTQELLCVIYQLMRNGSIP
                     SVPLFISGLGKSITEIYQDHRRYLRPQASILSFDLFSVLGYLDDLSIQSLLQEPCIIV
                     ATNGMMVENTPSARIARWMVQDERHGIFFCGYIDPDTLGYRVFHAAIGDALSFSPEGE
                     PVTVKNPHIRRFYLSSHADRYELLEVARRIKPCNVVLIHGEKEGIEYMQRELEGEFQV
                     LIGERGKTIALD"
     gene            complement(5572..6966)
                     /gene="gltX"
                     /locus_tag="VEL68_22705"
     CDS             complement(5572..6966)
                     /gene="gltX"
                     /locus_tag="VEL68_22705"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXZ38857.1"
                     /translation="MSQVILRFAPSPTGFLHIGGARTALFNWLLARNRGGRFILRIED
                     TDQVRSTRESIDAILEGMTWLGLDWDEGPVYQTDRMAMYREHAERLLHENKAYYCYCT
                     PEELEEKRQKALQEKRKPKYDGTCRGLQAPVPGRSPAIRFRAPQEGKTVLPDLIKGTV
                     EFENAEMDDLVILRSDGWPTYNFSVVVDDATMGITHVIRGDDHVNNTPRQILLYQAFG
                     YPLPQFAHVPMILGADKARLSKRHGATSVMAYKDMGYLPQALVNYLVRLGWSHGDQEV
                     FTMGELIQKFSLENVGKSAGVFNPEKLNWLNGVYIRQEKPETLAELLLPFLERKGLKP
                     RSMGWLVEIVKTLQERSKTLVEMADMAEFYLRDDFQVDEKAERKHLLPAIKEPMEIFL
                     GRLESVAEVNEKSLEEIFKEIVAQKNMKLGALAQAVRVALTGKSVSPGIYEVMRILGK
                     DEVLKRLSKALKKL"
     gene            7324..7533
                     /locus_tag="VEL68_22710"
     CDS             7324..7533
                     /locus_tag="VEL68_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017007742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXZ38858.1"
                     /translation="MAVGEGQVKWFNEKKGYGFIQQENGQDLFVHYTAIQGDGFKTLA
                     EGQKVRFEVEDTPKGPKAKNVQVIQ"
     gene            complement(7655..8263)
                     /locus_tag="VEL68_22715"
     CDS             complement(7655..8263)
                     /locus_tag="VEL68_22715"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HXZ38859.1"
                     /translation="MATAKSKKKKSGSAKKPVPTLKKKKAPKRPVKKAAQKKVLRKKE
                     RTMIKAVKPAERMAVPKAKDEKEEIRKTLLEMREKLLSGISENPIPEALISQSDIGDL
                     IDQAGDERDRELSLLLSGRDKEKLLAINEALEKLKDATYGDCEECGERIGAGRLKVMP
                     LARFCVSCQAKLEKEMTLQKREEEDLNLRGLAYSSSAEEEEA"
     gene            complement(8479..>9068)
                     /locus_tag="VEL68_22720"
     CDS             complement(8479..>9068)
                     /locus_tag="VEL68_22720"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HXZ38860.1"
                     /translation="DEEKVCKYLDLPIQHVDEGILKAMNRPPLKRGMPDLIDWVRREI
                     PGVVLRTSLIVGFPGETEQSFRRLLEFVQEAQFDRLGVFSYSPEEGTPAAALKDQVQD
                     RIKLQRSRQIMRLQKRISLKKQKALIGSRLPVLVERPGSSPEFLWEGRTQGQAPEVDG
                     VTFLTKGRARAGDMVDVRIQDAGPYDLFGEILGPA"
BASE COUNT         1902 a         2573 c         2350 g         2243 t
ORIGIN      
        1 ttcagcagga tcggttctgg cccagggcaa gtatcccacc aaagccattg atatcatcgt
       61 tccctatgct cccggcggcg ggaccgacat catgtatcga aatatcgaga aaatcatcag
      121 ccagtacaag ctggtttccc agccggtcaa catcgtaaac aaacccgggg gaggggggtc
      181 gatcggaaaa gccttttgcc tttcccgccc gccggatggc tactccttca cgtgcttcga
      241 cctgggaacc gtgtctcatc agatcgacgg gaaagccaaa tgggactacc gcaaggattt
      301 ttcctacatt gccaggatcg tcagcgatat caacctgatc atcgttcgaa ccgactcgcc
      361 catcaacaat gcccaggatt tggtggaaac tgttaagaaa aaaggaccca aatcggtatc
      421 catcgggggc accgcgatcg ggggcccgga tcaaatcggc cacttcgtgc tcaacaaggc
      481 caccaagcag cagttcaact acgttcctta caacagcggc ggggaggtgg taaccaacct
      541 cctgggaggg cacatcgatg ccgcctgggc caaccccaac gagtgcatcg gccagctgga
      601 ggccaagcag gtcaaaatcg tcggggtgtg caccgaaaag cggtcgccca tgttccccaa
      661 ccacccgact ttccgggagc aggggtatga tgtgatcagc gtccagaccc gctctatggt
      721 cggcagggcc gggatcccga aagaagcaat cgattattgg gtggcgatcc tggacaaggt
      781 ccggaatacg ccggaatgga agaatttctt gaagaccagc ctgctggatg acggctggct
      841 ggtgaaggaa gaattcttca aggatgcgga aaacgactac aacatggtca aaccgatcat
      901 ggatgaactg ggactttcca aaaaataagt tacgagtttc aggttgcaag tttcaagtta
      961 agggttcctg gccttggacc ttgcgccttt tttggtttga aaagttgaat gaaaaataaa
     1021 gatttctata gcgggttgat cctgctgatc ttgagcggga cgatcttcgt tcttatcctc
     1081 aggagtccgg ctcccccgga ctcctcaggc ccaggtccct ttttcctgcc ctttatttcc
     1141 gcattagtca ttgccggcct gtcaattgct cttttgctcc aaagccaaaa acaaccggag
     1201 acgggggcag cggcacccca aacaaagcgg atgtggaaca ggctcatctg gattgtggtc
     1261 tggtgctttg cttatggatt cagcatcgag aaattgggtt acctgttatc gacgggggtg
     1321 gttacttttg ccttgttggc ctattttaac cggcggaaat ggattctcaa cctgctcttc
     1381 tccctgttta cccctatttc catctatatt ttattcgaca ctctgctaaa agttcccttg
     1441 cccaaaggct ggtttgggtt ctgacggagg gggccttgga aatcctgcat aaccttttgt
     1501 acggattcac ggtcgccctt tcccccgcga actttctcta ctgcttcctg ggtgtcttca
     1561 tcggaaccat cgtcggcgtt ctgccggggc tgggggtcat gaacgcggtg gccattctta
     1621 tccccatgac ctttgggatg aaccccacgg cagccatgat catgttgtcc ggaatttatt
     1681 atggggccat gtacgggggg tctaccacgg ctatcctgat caacaccccc ggtgaagcgc
     1741 ctgcgatcat gaccacattg gacggttacc agatggccct tcagggaagg gccggcccgg
     1801 ccctcggcat cgctgctttt ggatctttca tcgccgggac ttttgccacg gtcatgctca
     1861 tggtcctggc cgaacccctg gtgaaggtag ccttgagctt cggcccgacc gaatattttt
     1921 ccatgctcat tctggccctc tgcaccctcg ggggcctgat gggggaatcg gtcatcaaag
     1981 gtcttctcat ggccgccttc ggaatttttc ttggcaccgt gggaatcgac ccggtagccg
     2041 gggtggaccg gtttacattc ggaatccccg attttatgga cggaatcggc tttcttcccg
     2101 tggccatcgg gctctttgcc attgccgagg ttttgctcac agccgaagtg gagatgatga
     2161 ggcccgtggt agaaaagatc aaaggcctgc tgccctcttt aaaagactgg atggagagta
     2221 aatgggccat aacccggggg acggtgatcg gatttttgat cggatgcatg cccggagggg
     2281 gcgccaccct ggcgtccgtc gtttcttacc tggttgagaa aaaattctcc aaaaacccag
     2341 agaggttcgg caagggagcc atcgaagggg tcgccgggcc agaatccgcc aacaacgccg
     2401 ccagcggtgg cgccatggtt cctctcttga ctctgggtat tccgagcacg ggagtctccg
     2461 cggttctctt gggtgccctg atgctctacg gactgcgccc cggtccgctt ctttttcaga
     2521 atagcccgga gctggtgtgg gggctcatcg ccagccttta cataggcaat tttctcctcc
     2581 tcctcattaa tcttcccctg attggcatct gggtaaggat gctgaaggtt ccctttcccc
     2641 ttcttcttcc cttcatcctg gtcatcgcca tcaccgggac ctatgccacg aacaacagtg
     2701 tgaccgaggt ttacgtcacc ctggtcttcg gggtgatcgg atatttcatg aaacacgccg
     2761 atcttcccgc agccccggtg atcatgggga ttgtcctcgg tcctctgctg gaaacccact
     2821 tccgccgggc tctgattatc tccaatggct cctacgccac ttttttcacc caccccgtct
     2881 ccgccatttt tctgtgcgcg gccgttgttt cgattcttta tccgctcatc aaatggggaa
     2941 taaaaaggaa gaacgtcacg cccgaggcgt aactcaagga tcgccgcgcc ttggtcgcgg
     3001 cttgaggagc ggcccgaagg gcctcgagga tcgaccctcc tgcggcgggc cttgaggatc
     3061 gcttcgcttg aggcaaatgc caaaaagttc tagcctcaag ggcgaagccc ggccctagag
     3121 tcaagccgga ggcttgacgg tcctcaaggg agcgcagcga ccgatcccta aagccccttg
     3181 ggccgatctt tccaggttga atttcatccc tgactatgtg atactgaatt aaaaaaagcg
     3241 aggtttgaag gatgggcctg atccgtcttc ttttcacgaa tccattcgct tttcttctgc
     3301 tcatcatccc ccttctctac tccatcatca tccatgagct ggcccacggg tgggtagcga
     3361 acaaaatggg ggactccacg gcgaaatggc tggggaggct cacccttaac ccgatgagac
     3421 acctggaccc catcgggacc ctgatgctct tcttcttcgg cttcggctgg gccaaacccg
     3481 ttccggtgaa cttcaacaac ctccacgacc cccgcaaacg attggatctg gtttccgcgg
     3541 ctggaatcat tgccaacacc cttctcgctt ttttcgctct cctcatcctc cgggtgttca
     3601 atcctgcccc cttcggggcc atctccacct tcctgattta catggcccag atcaacatca
     3661 tgctggcggc ctttaatttg attcccattc ccccgctcga cggttccaag atcctcatgg
     3721 ggttctcctc cccccaattt caatacaccc tggcccgtct ggaaccttac ggttttttta
     3781 tcatcattgc cctgctctac ctgaacatcc tgaacccgct catcgccctg ttcgaatgga
     3841 tcatcaccgg aatcatcagc ctcctgcttc cttgaacgga cccgggtgag ggaggaatgg
     3901 cctggacgtt tacagtcctc ggcggcggtc aggaaatcgg agccaattcc tatctcttaa
     3961 gcctttccgg ggtgcccctc ctcctggacg ccggtctcca tcccaaaaaa tacgggttcg
     4021 aaagcctacc tgattacgac ggaatcgaac gggagctgga ggctattttc ataacccacg
     4081 cccacctgga ccacgtgggg tccctgccgg tcatcaacca ccgccatccc caggctcccg
     4141 tttttgcaac cctccccacg aaagatatcg ccctgcggat gctgcacaat accgtaacgg
     4201 tgatgaatat cttccgggag gaaaaagggc tgatggaata tcccttcttc gaccatgaag
     4261 acctctggcc tctcgaaggg gaaattatgg gactggatta tagaatcgcg cggagttcat
     4321 ccgccccggg aaaacagatt tacagcgagc agctgcatct acgggggggg attcaggccc
     4381 gttttttgcc cgcgggacat gtcctgggag cggggggaat tctcttccgc caggggaggc
     4441 gcttccttta ttacacgggg gatatttctt tgcaagcgca agtcctgatt cccggagcgg
     4501 tcctcccgga tgagaggatc gacactctga tcctggagtg cacctacggc aatgaccctg
     4561 cataccattc tcggaaccgt catcaggagg tcgagtcttt cgcgcgggaa gcggcatcga
     4621 ttctcgccgc gggaggctgc atactcatcc ccgcctttgc cctcgggaag acccaggagc
     4681 tattgtgcgt catctatcaa ctgatgcgca acgggtccat cccttcggtc cccctcttca
     4741 tttccgggct ggggaagagc atcaccgaga tttaccaaga ccatcggcgt tatcttagac
     4801 cccaggcctc cattttgagc ttcgacctct tctccgtcct gggatacctg gacgatctct
     4861 ccattcaatc ccttcttcaa gaaccctgca tcatcgtggc caccaacggc atgatggtgg
     4921 agaacacgcc ctcagcccgg atcgcccgct ggatggtcca ggatgaacgg catggaattt
     4981 tcttctgcgg ttatattgat ccggataccc tgggctaccg ggtattccat gctgccatcg
     5041 gcgatgctct ttccttttcc cccgaggggg aaccggtgac cgtaaaaaac ccccacatac
     5101 ggcggttcta tctctcctcc catgccgacc ggtatgaact cctggaggtt gcgcgcagga
     5161 tcaagccctg caatgtcgtc ttaatccacg gggaaaagga aggaatcgag tatatgcaga
     5221 gagagctgga aggggagttt caggttttga tcggggagag ggggaagacg atcgcattag
     5281 attagtttcg tgttccgggt ttcgggttaa taaaaagaca cttactaagg agtccattat
     5341 agtctaaaca tcgttgatta gaggacgttg cctttttaaa acctcgaact ggactccggc
     5401 tttctccgga gtgacgaatt acagaaaggc ctgatccttt gttgtcattc cggcggaagc
     5461 cagaatccag gatcaatcaa agaaaaatac ctatccacat aatgatggcc tcattattaa
     5521 agccatttct agccttcgtg aaacccggaa gctgaaactc gaaacccgaa actatagttt
     5581 cttgagggct ttggacagtc gtttcaggac ttcgtccttt cccaggatcc tcatcacctc
     5641 atagattccg gggctgactg attttccggt gagggccacc cggacagcct gagcaagagc
     5701 ccccagcttc atatttttct gggcgacgat ctccttgaat atttcttcca gggatttctc
     5761 gtttacttcg gcaacggatt ccagcctgcc caggaatatc tccatcggtt ctttgatggc
     5821 cggaaggaga tgcttccttt ccgctttttc gtccacctgg aaatcatccc ggaggtagaa
     5881 ctccgccatg tcggccatct ccaccagggt cttggacctc tcctggaggg ttttcacgat
     5941 ctccaccagc cagcccatgg acctgggctt cagccctttc ctttccagga aggggaggag
     6001 cagctcggcc agggtctccg gcttttcctg gcggatgtaa accccattca gccagttgag
     6061 cttttccggg ttgaatacgc ccgccgattt ccccacgttc tcgagggaaa atttttggat
     6121 cagttccccc atggtaaaca cttcctgatc cccgtgagac cagcccagac gaacgaggta
     6181 attcaccagc gcctgaggca gatatcccat gtccttgtag gccatcaccg aggtggcgcc
     6241 atggcgtttg gagagccggg ccttatccgc gcccaggatc atgggcacat gggcgaattg
     6301 aggcagaggg tatccaaaag cctggtaaag gagaatctgc cggggggtgt tattcacgtg
     6361 gtcgtccccc cgaatcacgt gggtgattcc catggtggcg tcgtcgacca ccacgctgaa
     6421 gttgtaggta ggccatccgt cgctgcgcag gatgaccaga tcatccatct ccgcgttctc
     6481 aaattccacc gtccccttga tcaggtcggg gagaacggtc tttccctcct ggggggctct
     6541 gaaacggatg gccgggcttc ggccggggac aggcgcctgc aacccccgac aagtcccgtc
     6601 atatttgggt tttcgtttct cctgaagggc tttctggcgc ttttcttcga gttcttcagg
     6661 ggtgcagtag cagtaatagg ccttattctc gtggaggagc cgctcggcgt gctcccggta
     6721 catggccatc cggtccgtct ggtagaccgg tccctcatcc cagtccaacc ccagccaggt
     6781 catcccttcc aggatggcgt caatggattc cctggtggaa cggacctggt ccgtgtcttc
     6841 gattcgaaga ataaatcttc ccccccggtt ccgggcgagc aaccaattaa aaagagcggt
     6901 ccgggcccct ccgatgtgca ggaaaccagt ggggctgggg gcaaaacgca agatcacctg
     6961 gctcattatt ttctcctgcg gtcctttttc attttatctt agcttttcgg caccttaggg
     7021 tcaagaggga taatatcgcc cggcccttcg ggcctcgagt catgccaaag gcatgagggt
     7081 cttcgaatcc cctcgggggg cgggatagtc ctcaatcgct ggtggttact ttttttaaat
     7141 ttttttattg acaggatttt ttgttcggcg tactataaat ttggatgagt cggatggtag
     7201 ttttgggaaa cagataaatc ggccattggt ttggttttcg tactccaaaa cccaatggcc
     7261 ttttttgttt ggccgaagcg atatcctgac ttattctaga agcatgaagc agaagggagg
     7321 gatatcgctg tgggtgaagg acaggtaaaa tggttcaacg aaaagaaggg ttatggcttc
     7381 atccagcagg agaatggaca ggacctattc gtccattaca ctgctattca aggtgacggc
     7441 ttcaaaaccc ttgccgaagg ccagaaagtc cgcttcgaag tcgaagatac ccccaagggg
     7501 cccaaggcca aaaacgtcca ggtcattcag taatttttaa acctggcatg atctaaaacg
     7561 gcacctcgcg atccgcaggg tgccgttttt ttctcctcaa aaaaaatccc ggcatagccc
     7621 gggattttca agaatcgggg ataaggggaa aaaatcaagc ttcctcttcc tccgcactgg
     7681 atgagtaagc aagccctcga aggttaaggt cttcttcctc tctcttctgc aaggtcattt
     7741 ccttttccag cttggcttgg caggaaacgc aaaatctcgc caggggcatg accttcaggc
     7801 gtcccgcccc gattcgttct ccgcactctt cacaatcccc ataagtggcg tctttgagtt
     7861 tttccagggc ttcgttgatg gccagcagtt tttctttatc tctgccggaa aggagaaggg
     7921 aaagctcccg atcccgttca tcgccggcct ggtcgatcag gtctccgatg tcggattggg
     7981 aaattagagc ttccggaatg gggttttcgg aaattccgct aagcagcttt tcccgcatct
     8041 ccagcagggt cttcctgatt tcttcctttt catctttcgc cttgggaaca gccatccttt
     8101 ccgcaggctt cacagccttg atcatagttc tttccttctt ccgcaatacc tttttctggg
     8161 ccgccttttt aacgggcctt ttaggcgcct ttttcttctt gagggttggg accggctttt
     8221 tggccgagcc gctttttttc ttcttggatt tcgcggtcgc catggcgggg ttcctccctt
     8281 tccgagaaag atgaacgaat tttctctatt aagggaacat aatctaccgt ttttcccccg
     8341 gttgtcaaga ataaaaatca aatttccggg aaaaataaac cggctcccgc cgaaaatcga
     8401 aggccagtca aaaaactctt tctccccatc ggctggtggg gggaaaatct tcaattttcc
     8461 ggaggagcta gtgaagcttc aggccggccc gaggatttct ccgaaaaggt cgtaaggccc
     8521 tgcatcctgg attcgcacat ccaccatatc cccggcacga gctcttccct tcgtcaggaa
     8581 ggtgactcca tcgacctccg gggcctgccc ctgagttctt ccttcccaca gaaattcagg
     8641 agaactgccg ggccgttcca ccaggaccgg aaggcgggaa ccgatcaagg ccttttgctt
     8701 tttcagagaa attctcttct gcaggcgcat gatttggcgg gaacgttgaa gtttgattcg
     8761 gtcctgaacc tggtctttca aagccgctgc cggagtccct tcctccgggg aataggaaaa
     8821 aactcccagg cggtcgaatt gagcctcctg gacaaattcc agaaggcgac ggaagctctg
     8881 ttccgtctcg ccggggaagc ccacgatcag agaggtgcgg aggaccactc cggggatttc
     8941 ccttcgaacc cagtcgatga ggtccggcat tcctcttttc agcgggggac ggttcatggc
     9001 cttcagaatc ccttcgtcca catgctggat aggcaaatcc agatacttgc agaccttttc
     9061 ctcatcgc
//
LOCUS       DASPRR010000619         6272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1926261, whole genome shotgun sequence.
ACCESSION   DASPRR010000619 DASPRR010000000
VERSION     DASPRR010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926261"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /gene="kdpB"
                     /locus_tag="VEL68_22725"
     CDS             <1..1784
                     /gene="kdpB"
                     /locus_tag="VEL68_22725"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HXZ38861.1"
                     /translation="TQAKKLSRGYSVEANLKPESRSYSPVSSTELRKGDLYFVEAGDT
                     IPADGEIVEGVASVDESAVTGESAPVIRESGGDRSAVTGGTRILSDWLIVRVTMDPGE
                     GFLDRMISLVEGAKRQKTPNEIALDILLAGFTIIFLVVCATLLPFSLYSVKVAGQGIP
                     VTITVLVALLVCLIPTTIGGLLSAIGIAGMDRLIRRNVIAMSGRAVEAAGDVDVLLLD
                     KTGTITLGNRQAVELIPISTLDIRELAEWTQLASLADETPEGRSIMTLVKEKYGLRGR
                     TVRPGEGPEMRFIPFSAQTRMSGVDMDGVKIRKGAFDVIAQYIEGSGNQVSREIRDAV
                     ERISLSGGTPLVVARNGEAAGVVHLKDVVKGGIRERFSQLKRMGIRTVMITGDNPVTA
                     AAIAAEAGVDDFLAQATPEAKLKLIRQHQAGGRLVAMTGDGTNDAPALAQADVAVAMN
                     TGTQPAREAANMVDLDSNPTKLIEIVEIGKQLLMTRGALTTFSISNDVAKYFAIIPAA
                     FASTYPALAALNFMHLQSAILSAVIFNALIIVALIPLALKGVPYRAVGAARLLRDHLL
                     IYGIGGLIAPFIGIKLIDLLLVAAGLA"
     gene            1820..2446
                     /gene="kdpC"
                     /locus_tag="VEL68_22730"
     CDS             1820..2446
                     /gene="kdpC"
                     /locus_tag="VEL68_22730"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HXZ38862.1"
                     /translation="MFAILRPALMVFITLSILTGALYPLFVTGVAQLIFPVQANGSLM
                     ERVGKPIGSELIGQPFDDPKYFWGRLSATPDFPYNSASSSGSNLGPSNPALVEEVKAR
                     VEALQKADLGNKSSIPIDLVTSSGSGLDPHISPAAALYQASRVARFRGLSEERVRTLV
                     DQFTEPRQWGFLGEPRVNVLRLNLALDGMQGQSKSSSPPFSAGGIGVK"
     gene            2553..5228
                     /locus_tag="VEL68_22735"
     CDS             2553..5228
                     /locus_tag="VEL68_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HXZ38863.1"
                     /translation="MNGENRPDPDSLLARVEAEERQKALGKLKIFLGYAAGVGKTYSM
                     LEAAHSRRAEGVDLVAALVETHGRGETEALLQGLEIIPRKPVEYRGVKLSEMDLDGVL
                     ARKPQLALVDELAHSNAPGSRHPKRWQDIEELLKAGIDVYTTLNIQHLESFRDIIAQI
                     TEVVQRETIPDRVLDEASEIEVVDLPPEELLLRLREGKVYIPEQAARAIENFFEPGNL
                     IALREITLRRAANRVDEQMREYLQAYPMEGLWPVTERLLVCISGSPTSQRLIRTARRL
                     GEELKAEWSVLYVETAEDDKLTRENRERVWSDLRLAESLGAKEVSTVKGDSVLEAVID
                     YARKHQISKIIVGRPTRSSWRGWMRGSVVDQILRKSPKMDVYVVSDAGTGENGKAYSP
                     RSAPVPWQSYFTSLLLVAAASLASGVAFPFLTPINLMILYLLAVVVAALRLGFKPALL
                     TAFLGVLSFDFFFIPPYYSFAVADTEYFITFAGLLIVGAVISTLVARARKQTETIRTR
                     EMQTAHLYALSRGLASAVGLQDILNTLIRHVSETLPGQVAIFTPEGESLTLAAGSPRI
                     SPGEKEKSVALWAYRNGQMAGRGTETLSSSEFLYLPLKTGARAVGVMGIKMDLAGEEL
                     APERRRLLEAFSNQAALAIERAVLAREAEQAQLHQATERLERSLLNSISHDLRTPLSS
                     IMGTLSSLREEGNAPGDESRRELLDLAWEEAERMNRFVGNLLDITRLEAGVLKIKKEP
                     YDLQDLLGSCLASLESRLKGRKVKIQVPPDLPLIPMDSVLMAQVLINLLENALKYSPP
                     DGIIEVSGRVRETRVEIEVADQGPGIPQEFQNQIFNKFFRFRRTEEVGGTGLGLAISK
                     GIVEAHQGKIWAENLPQGGFKIVFALPIAPPGGGI"
     gene            5253..5960
                     /locus_tag="VEL68_22740"
     CDS             5253..5960
                     /locus_tag="VEL68_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXZ38864.1"
                     /translation="MTPGSSERNLPRVLVVDDERAIQRFLKNALSQAEFSVHIVGSGK
                     EALTAAVAIRPDLIILDLGLPDLDGLEVLHRLRQWTKVPVIVLSVRDREDDKIAALDS
                     GADDYVTKPFGTGELLARMRAALRKSIQESPLPVYKVDSLEVDLEHRRVVAEGEEVQL
                     TPTEYDLLRLLVTHAGKVLTHNQILRQIWGPAYVEQPHLLRVNISNLRRKIEPEPTRP
                     RYVLTELGVGYRLKLKT"
     gene            complement(6009..>6272)
                     /locus_tag="VEL68_22745"
     CDS             complement(6009..>6272)
                     /locus_tag="VEL68_22745"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HXZ38865.1"
                     /translation="AFHAALAKASHNSILARYLAELRHPMKSMMEQKASVFIDYDRVA
                     EQHETIVQAIEAHNPRKAQSALRNHLEWAGERLKAVVLKKESK"
BASE COUNT         1342 a         1748 c         1897 g         1285 t
ORIGIN      
        1 aaacgcaggc taagaagtta tccagggggt attctgtcga agcaaatctg aaaccggaga
       61 gccggtctta ttccccggtc tcctccaccg aattaagaaa aggggacctg tatttcgtgg
      121 aagcgggaga tacgattccg gcggatgggg agattgtgga aggggtcgcc tcggtcgacg
      181 agagcgcggt aaccggggag agcgctccgg tcattcggga atcgggagga gatcggagcg
      241 cagtcaccgg cggcacccgg attctctccg attggctgat cgtgcgggtt accatggacc
      301 ccggcgaagg ttttctcgac cggatgatca gcctggtgga aggagcgaag cggcagaaga
      361 cccccaacga aatcgccctg gacattctgc tggccgggtt tactatcatt tttctggtgg
      421 tgtgcgccac cctcctgcct ttttcccttt acagcgtgaa agtcgcgggc cagggaatcc
      481 cagtgaccat aaccgttctg gtcgctttgc tcgtctgcct cattcccacc acgatcggcg
      541 ggctcttgag cgccattggt attgccggca tggaccgcct gatccggcgc aacgtcatcg
      601 ccatgtcggg aagggcggtg gaggcggcgg gcgacgtgga tgtgctcctt ctggacaaaa
      661 ccggaaccat taccctgggg aatcgccaag cggtggaatt gatccccatc agtaccctgg
      721 acatacggga actggcggaa tggacccagc tcgcctccct ggcggacgag actccggaag
      781 gacggagcat catgactctg gtgaaggaga aatacgggct gcgggggcgg acggtgagac
      841 ccggtgaggg cccggagatg cgtttcatcc ccttcagcgc gcagacgcgg atgagcgggg
      901 tcgatatgga cggggtgaaa attcgcaagg gagctttcga cgtaatcgct caatacatcg
      961 agggcagcgg gaaccaggtt tcgcgagaaa ttcgagacgc cgtcgaacgg atctcccttt
     1021 ccgggggaac tccgctggtg gtcgcccgga acggagaagc ggccggggtc gtccatctca
     1081 aagatgtagt gaaagggggg atccgggaac gcttttctca gctgaaacga atggggatcc
     1141 gcacggtcat gatcaccgga gacaacccgg tgaccgctgc ggccatcgcc gccgaggcgg
     1201 gggtggatga cttcctcgcc caggccaccc ctgaggccaa gctgaagcta atccgccagc
     1261 accaggccgg tggacgtctg gtggccatga ccggggacgg aaccaacgac gctccagccc
     1321 tggcccaggc cgatgttgcc gtggccatga acaccggaac ccagccggcc cgggaagcgg
     1381 ccaatatggt cgacctggac agcaacccga ccaaactgat tgaaatcgtg gaaattggca
     1441 agcagctctt gatgacccgg ggggcgctca ccaccttcag catctccaac gacgtggcca
     1501 aatacttcgc catcatccct gcggccttcg cctcgaccta cccggccctc gcggcgctca
     1561 acttcatgca cctccagagc gccatcctgt cggcggttat cttcaacgcc ctgatcatcg
     1621 tggcgctcat cccccttgcc ctgaagggcg tgccctaccg ggcggtgggc gcggcccggc
     1681 ttctgcggga ccaccttttg atttatggaa tcggggggct gatcgcccct ttcatcggga
     1741 ttaaactgat cgacctgctc ctggtggcgg cgggattggc ctagcctctc ccggagggat
     1801 ttagagaaag gaatccaaca tgtttgccat tctcagacca gcgctcatgg ttttcatcac
     1861 cctcagcatt ctgacgggag ccctttaccc gctcttcgtc accggcgtgg cccaattgat
     1921 tttcccggtt caggccaatg gaagcctgat ggagagggtg ggcaagccga tcgggtcaga
     1981 gctcatcggc cagccattcg atgacccgaa atatttctgg gggcgcctat cggctacccc
     2041 ggatttcccc tacaattcag cctcgtcttc cgggtccaac ctagggcctt caaacccggc
     2101 gctagtggaa gaggtaaaag caagggtgga ggctcttcaa aaagccgatc ttggcaataa
     2161 atcatccatc cccatcgacc tggtcacttc ttcgggcagc gggctggacc cgcacatcag
     2221 cccagccgcg gctctgtatc aggcatccag ggtggcacga ttcagaggcc tgtcggaaga
     2281 acgggttcga accctggtgg accagttcac cgagccccgc caatggggtt tcctggggga
     2341 accgagggtg aacgttttaa ggttgaacct ggccctagac ggaatgcagg gccaaagcaa
     2401 atcatcttct cccccttttt ccgctggtgg aataggagta aaatgatttc aggcggtaga
     2461 agcggggccg ttgatttcgg caaaatttca gggaattgcc aagcatgacc ttggagcacg
     2521 ggtccacttc tgctttcccg cgggagtaaa ggatgaacgg cgagaatcga cccgatcccg
     2581 attccctctt agcccgggtt gaggccgaag agcggcaaaa agcccttgga aagctaaaga
     2641 tttttctcgg gtatgcagct ggggtgggca agacctactc catgctagaa gccgcgcaca
     2701 gccgcagggc agaaggggtg gatttggtgg cggcccttgt ggaaacccac ggacggggag
     2761 aaaccgaagc cctgctgcaa gggctggaaa ttatcccccg aaaaccggtc gagtatcggg
     2821 gggtgaaact ttccgagatg gacctggatg gggttctggc ccgcaagcct caattggccc
     2881 tggtggatga actggcccat agcaatgccc ccggttcccg gcaccccaag cgatggcagg
     2941 atattgagga gcttctgaag gccggcatcg atgtctacac caccctgaac attcaacatc
     3001 tggagagttt ccgggacatc atcgcccaga ttaccgaggt tgttcagcgg gaaactatcc
     3061 ctgaccgggt gttggacgag gcctcggaaa tcgaggtggt tgacctgccg ccggaagaac
     3121 ttttgctccg tcttcgcgaa gggaaagtct acatcccgga acaagccgct cgggcaatag
     3181 agaatttttt tgagccaggc aatctgatcg ccctgcgtga aatcactctg agaagggctg
     3241 ccaaccgcgt cgatgagcag atgcgcgaat acctccaggc ttacccgatg gaagggcttt
     3301 ggccggtcac cgagcgcctc ttggtgtgta tcagcggcag tcccaccagt caacgactca
     3361 tccgtaccgc gcgccgcctg ggggaagaat taaaggccga atggtcggtc ctttacgtag
     3421 aaacagcaga ggacgacaag ctgacccggg aaaatcggga gcgggtctgg agcgatttgc
     3481 gtctggccga atccctcggg gccaaagagg tatccacggt gaagggggat tccgtcctgg
     3541 aggcggtgat tgattatgcc cgaaagcatc aaatctctaa gatcatcgtt gggcgtccta
     3601 cccgatcctc gtggcgagga tggatgagag ggtccgtagt ggaccagatc ctgaggaaaa
     3661 gccccaaaat ggatgtctac gtcgtaagcg atgcgggaac cggagaaaat ggaaaagcct
     3721 attccccgcg atcggctccg gttccctggc agagttactt caccagtttg ctgctggtag
     3781 ccgccgcaag cttggccagc ggggtcgcct tccccttcct cacgcctatc aacctgatga
     3841 tattatacct cctggcagtg gtcgtggctg cactccgctt gggattcaaa ccggccttgc
     3901 taactgcctt cctgggcgta ttgtcctttg acttcttttt catccccccc tattacagtt
     3961 ttgccgtggc cgacacggag tatttcatca cttttgcggg tcttctaatc gtgggggcgg
     4021 tgatcagcac tctggttgct cgagcccgaa aacagaccga gacgatccgg acccgggaaa
     4081 tgcagactgc acacctgtat gccttgagcc gcgggctggc ctcggccgtc ggtctgcagg
     4141 atattttgaa tactttgatt cgtcatgtga gcgagacctt accagggcag gtggcgatct
     4201 ttaccccaga gggagaatcg ttgaccctgg cggccggctc gccaaggatt tccccagggg
     4261 aaaaagaaaa atcggtggct ctctgggctt atcgcaacgg gcagatggcc gggaggggaa
     4321 ccgagaccct ttcctcttcg gaatttctgt accttccctt gaagaccggg gcccgggccg
     4381 ttggggtcat ggggatcaaa atggacctcg cgggtgagga gctggcgccg gaacgccgtc
     4441 ggctcctgga agccttttcc aaccaggcgg cactggccat cgaaagagca gtccttgccc
     4501 gggaagcaga acaggcccaa cttcaccagg ccacggagag attagaacgg tctctgctca
     4561 actcgatctc ccacgatctg cgaacgcccc tatcttccat catgggcact ttgagcagcc
     4621 tgcgggaaga agggaatgct cccggggacg agtccaggag ggagctgctc gacctggctt
     4681 gggaagaggc ggagcggatg aaccgttttg tgggaaacct gctggatatc acccgtctgg
     4741 aagccggggt tctgaaaatc aaaaaggaac cctacgattt gcaggacctt cttggttcct
     4801 gcctggcttc cctggaatcc cggctgaaag gaagaaaagt caagattcag gttccccccg
     4861 accttcccct catccccatg gactccgttc tcatggccca ggtcttgata aacctcttgg
     4921 aaaacgccct gaaatattct ccgcccgatg ggatcatcga ggtgagcggc cgggtccggg
     4981 aaacccgggt ggaaatcgaa gtggcagatc aggggcccgg aattccccag gagttccaga
     5041 accaaatatt caataagttt ttccggttca ggcgtacgga agaagtcggt gggaccggct
     5101 tgggtctggc catcagcaaa gggatcgtgg aggcccatca gggaaaaatc tgggctgaaa
     5161 accttcccca aggagggttc aaaatcgttt ttgctcttcc tattgctcct ccgggagggg
     5221 gaatatagat ttccaactgg ggagcctcaa agatgactcc agggagcagt gaaagaaatt
     5281 tgccgcgggt gctggtggtc gatgatgaaa gagccatcca gcgtttcctg aaaaatgccc
     5341 taagccaggc ggaattttcc gttcatatcg tgggaagcgg gaaagaagcc ctgactgcgg
     5401 cggtcgccat ccgccccgac ctcattatac tggacctagg tttgccggac ctggatggac
     5461 tggaagtcct ccatcggttg cgccagtgga cgaaggtgcc ggtgatcgtt ctgtcggtcc
     5521 gggaccggga ggatgacaag atcgccgctt tggattccgg ggccgatgat tatgtgacca
     5581 agcctttcgg aactggagag cttctggcga ggatgcgggc agcccttaga aaatccattc
     5641 aggagtcgcc ccttcccgtt tataaggtcg acagcctgga ggtggacctg gaacaccgcc
     5701 gggtggtggc cgaaggggaa gaggtgcagc tgacccccac ggagtatgac ctattgcgtc
     5761 tgctggtcac ccatgcaggc aaagtactga ctcacaacca gatcctccgg cagatctggg
     5821 gccccgccta tgtggaacag ccccatttgc tccgggtcaa catcagcaac ctccgccgca
     5881 agatcgagcc cgaacctacc cgcccccggt atgtcctaac ggagctcggc gtagggtacc
     5941 gacttaaatt gaagacctag accttgaatt tgaaagagaa gcggctttcc gcgattcaat
     6001 ttacgagatt acttcgattc ctttttcagg accacggcct tgagtctttc tccggcccat
     6061 tccagatgat tcctgagagc ggattgggct tttctgggat tgtgtgcttc gatggcttgg
     6121 acgatggtct catgctgttc ggcgacgcgg tcataatcaa tgaaaaccga ggctttctgc
     6181 tccatcatgc ttttcatggg atggcgaagt tccgccaggt agcgggctaa gatactgttg
     6241 tgactggctt tggccaaggc ggcatggaaa gc
//
LOCUS       DASPRR010000620         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1926593, whole genome shotgun sequence.
ACCESSION   DASPRR010000620 DASPRR010000000
VERSION     DASPRR010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926593"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VEL68_22750"
     CDS             complement(<1..864)
                     /locus_tag="VEL68_22750"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXZ38866.1"
                     /translation="MPGPLEGVKVLSFARNLAGPYCTMILCDLGAEVIKIEEPGKGDT
                     ARFVGPHIQGISSYFLSINRGSRSITLNLREERAKEVVFRLVDRVDIVVENFRPGVMK
                     RLGFSYETISARNPKIVYASISGFGQKGPYSAKPAYDMIAQGVGGTVSITGEPGRPPV
                     RVGFSTGDIGAGLFAATAILAALHEREKSGKGQWIDVAMVDCQVAFCENACARYFATG
                     EIPKPLGTRHPLLTPFQVFPTKDGYMVLITVSEEDWQKFCKLTGRDDLASDPRFQGHP
                     ARIQNYSVYEPL"
     gene            complement(1003..>2582)
                     /locus_tag="VEL68_22755"
     CDS             complement(1003..>2582)
                     /locus_tag="VEL68_22755"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXZ38867.1"
                     /translation="AIFFCLIMGWEGLKMAAVALRNWEVSPSLLRTPVFIPQIFIPLG
                     SVLLTLEFIRLLKNLFRELPVARKPGKGSLLLQFWITALFAAGLAISFVLLKTSMHLG
                     LLVLFFVVLLCGIPVAIALALFGVFGFYFLFGGSSMLVQIPVMAYSTLDSFVMVALPL
                     FILTSCVLRLGGVGGRIYKFANVLVRHLPGGLGIASIIFCGLFAAMTGSSVAVAAAVS
                     MIALPEMLARGYNRKFTIGLLAAGGTLGILFPPSIPMMLYASMTNESLGALFMGGVIP
                     GLILSGMFCLYVALVAGRDKNVQRQPKASLSEILSGTKEAAGGLITIIIIMGGIYSGI
                     ITPTESGGVAGVYSIILCLFLYRTLSLQNMRKALLDAARISAMIMLIVIGANISGQVI
                     LMAQISQNILLWVKSMELPAWVIILCINIFLIIMGGPLEAISILVITLPILYPLMTGL
                     GFSGVWFAIVMVINMELALISPPEGLNLFILQDMAKSTAGEVSRGVVPFLIIIGLFLA
                     LISAFPGLSTWLPSLMQ"
BASE COUNT          626 a          701 c          750 g          505 t
ORIGIN      
        1 gagaggttcg taaaccgagt agttttgaat cctcgccgga tgtccctgaa aacggggatc
       61 ggaggccagg tcgtcccttc cggtcaattt gcagaatttt tgccaatcct cttccgagac
      121 agttatgagt accatatagc cgtctttggt gggaaaaacc tgaaagggag ttaacagggg
      181 atgccgagtt cccagaggct tggggatttc tccggtggca aagtagcggg cgcaggcgtt
      241 ctcgcagaag gcgacctgac agtccaccat ggccacgtcg atccattggc cttttccgct
      301 cttctccctt tcgtgaagag cggccagaat ggcggtggcc gcgaacaggc cagcgccgat
      361 gtcgccggtg gagaaaccca cccggaccgg cggccggccg ggttcgccag tgatgctcac
      421 cgttcccccg actccttggg caatcatgtc ataggccggt tttgcagaat acgggccttt
      481 ctggccgaag ccggagatgg aggcatatac gatcttggga ttccgggcgg atattgtctc
      541 atagctgaat cccaggcgct tcatgacccc gggccggaag ttttctacca cgatatccac
      601 ccgatcgacg agcctgaaca ccacctcctt ggccctttcc tcccggagat tcaaggtgat
      661 gcttctgctc ccccgattga tgctcaaaaa gtaagagcta attccttgaa tatggggtcc
      721 aacgaagcga gccgtgtctc ctttccccgg ttcctcgatt ttgattactt ccgctcccaa
      781 atcacacagg atcatggtgc aataaggccc ggccagattg cgggcgaaac tcagaacttt
      841 cactccttcc agcggtcccg gcatattttg attctcctct ttggttctct tttgagcgct
      901 gcattcggag ctttttcgcc gtcttctcct ggagcgtctc actttcaaga gaaagtcaat
      961 tgatgtccta actacatagt tttcctttca cgaggcaagc tttcattgca tcaaggatgg
     1021 cagccaggtg gaaaggccgg ggaaggcaga aatcaaggcc aggaagaggc caataatgat
     1081 aaggaaggga accaccccgc gtgagacttc tcctgcggtg gatttggcca tatcctggag
     1141 aataaataga ttcaacccct ccgggggcga gatcagggcc agctccatgt tgatcaccat
     1201 gacgatggca aaccaaaccc cgctgaaccc gaggccggtc atgaggggat aaagaatggg
     1261 cagggtgatg accaggatgg agatggcctc taagggcccg cccatgatga tcaaaaagat
     1321 attgatgcaa aggatgatca cccacgccgg gagttccatg gattttaccc agagaaggat
     1381 attctgagag atctgggcca tgaggatgac ttgcccggaa atgttagccc cgatcacgat
     1441 cagcatgatc atagctgaaa tccgggcggc atccaggagg gcttttctca tattctgaag
     1501 ggaaagggtg cggtacagga aaaggcaaag gataatgctg tagaccccgg ccaccccccc
     1561 tgattcagtg ggggtgatga tcccggaata gatcccgccc atgatgatga tgatggtgat
     1621 cagccccccg gccgcttctt tggtcccgct taaaatttcg gagaggctcg ctttgggctg
     1681 tctttgaacg ttcttatccc ggccggcaac cagggccacg tacaggcaga acatcccgga
     1741 caggatcagc ccggggatga ccccacccat aaacagggcg cccagggact cattggtcat
     1801 tgaagcgtag agcatcatgg ggatactggg gggaaagagg atgcccaggg ttcccccggc
     1861 ggccagaagg ccgatggtaa attttcgatt gtatcccctg gccagcatct ccggcaaggc
     1921 aatcatcgaa acggccgccg cgaccgctac gctggagccg gtcatggccg cgaacagccc
     1981 gcagaagatg atcgaggcga tacccagacc gccggggagg tgccgtacca gcacgttggc
     2041 aaatttgtag attctgcccc ccacccctcc cagccggaga acacagctgg tcaggatgaa
     2101 gagaggcagg gccaccatga caaagctgtc caaggtggaa taggccatga ccgggatctg
     2161 aaccagcatg gaggagccgc cgaagaggaa atagaatccg aagaccccga acagcgccag
     2221 ggcgatggcc accggaatcc cgcaaaggag gacgacaaag aaaagcacca agaggccaag
     2281 gtgcatggag gtcttgagga ggacaaagct gatggccagc ccggcggcga aaagggcagt
     2341 aatccaaaat tgcaggagga gggacccttt cccgggcttt cgggcgacgg gcaactcccg
     2401 gaatagattt ttcagaagcc ggataaattc cagggtcagc aggacgcttc ccagggggat
     2461 gaaaatctgg ggaatgaaaa ccggcgttcg cagcagcgag ggagagactt cccagttccg
     2521 cagggcgacg gcggccatct tcaaaccttc ccagcccatg atcaggcaga agaagatggc
     2581 ca
//
LOCUS       DASPRR010000621         4596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2090841, whole genome shotgun sequence.
ACCESSION   DASPRR010000621 DASPRR010000000
VERSION     DASPRR010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090841"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1540
                     /locus_tag="VEL68_22760"
     CDS             395..1540
                     /locus_tag="VEL68_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ38868.1"
                     /translation="MDYELPENLRLMRDSARRFVKNELEPISQKVEEEEKIPESVVQK
                     MREMGFFGMSIPEEYGGMGLGTLGECVMNEEFGRVNSSFRSRFGTNNGIGCQGILIDG
                     TPEQKEKYLPRIASGEWTAAFALTEPNAGSDAANIQTKAEKKGDVYLLNGLKHFITNG
                     DIADVVTVMAVTDKTKGPRGVTAFIVEKTFPGYSVGKIDKKMGIHGNNTSEIVFEDCQ
                     VPVKNIIGLEEGRGFVTAMKVLEKGRITIAAVCVGTAQYCLDTSVAYSKQRVQFGKPI
                     CTNQAIQWMLADMAISIYTARQIVYNAAWCRDQKQRVTQQAAMVKVYCTEMVNRVVDS
                     ALQIHGGMGYMKESPIERIYRDMRLYRIFEGTSEVQRMVIARELLKD"
     gene            1640..3385
                     /locus_tag="VEL68_22765"
     CDS             1640..3385
                     /locus_tag="VEL68_22765"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HXZ38869.1"
                     /translation="MAGRRGSGKAGNGPVTVPIDNTGQAYLEVLRDRGIKYFFGNAGT
                     DFAPLIDGFARLAAEGKEYPKPVLVPHEFAAVSMAHGYAMVTGEPQVVMVHVNVGTSN
                     ALTGVMNASRANIPLIFTAGRTPITEGEVRGGRNLHIHWAQESFDQASMVREYVKWDY
                     ELRCFAQVEKVVDRALNMTMSEPKGPVYLSLPREVLAEAHDEFTYTPAGPRLYAKPGP
                     DAEALRQAALLLKNAENPLVITSMVGKNPAAVGQLVKLCESFALPVTIPMSNLYLNFP
                     TDHPMHLGFDPMKFVPTADVILVVEADVPWFPIRAKPKPEAKVIQLGIDPFFSRYPMR
                     SFPADIPLACDPEAGLKALREALEAQPPQSKRDIQNRYERLSQEHRTQRAGWKAAAER
                     LKSERPIDMEYVSYCVGQVRDAETILINEYDLRAHQVSLNEPGSFFSSPMSSGLGWCF
                     GAALGVKLAKPDTVPMVCMGDGTYHFTAATACHFMAQNLPVVLVVFNNQCWNAVKGSV
                     LDLYPEGWAVQKKHFVLCDLKPEPAYEKIVEAFGGYGERVEDPDKIIPALKRAVKAVR
                     QEKRQALLNVICKHP"
     gene            3578..4417
                     /locus_tag="VEL68_22770"
     CDS             3578..4417
                     /locus_tag="VEL68_22770"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HXZ38870.1"
                     /translation="MAPKIALPSEVAGWKWDGKGKSFNTKTVFEYIDGAAELYLAYGF
                     LGLKVWRYEKTGAPPINLEIYEQGSSEDAYGVFSFEQQDEPAGVGQGSEFGGGLLRFW
                     KGSYFVSVYAEGEGKGVEAAILDIGKAAAQAIPITGPEPKLVQFLPGKEFGLVDRSIR
                     FLKSHVLLNQRFFISHQNILNLSRKTDAVLAVYLRGGQKAHLLLIRYPSADEARISLE
                     SFRKAYMPDAGEKDRVKTEDRKWTFSRQQKDYLLLVFGAPTESDAESLLKATEEKLIT
                     GSR"
BASE COUNT         1133 a         1172 c         1344 g          947 t
ORIGIN      
        1 gcccaaacca gaaaagatat cgtaggggcg gtcccgcctt tagcgggatg gccgcccgca
       61 agatgggcgc ccgcgggggt ccccccacaa ttcattaccc cctcgcgggc gatttcattt
      121 atcggctttg ttcgaaaggt acatgattca tcgttctctc gggccaactg aacaaccgcg
      181 aaaaatcatg accgccacca gaggtggagg tatgaaaaac agtgaaacgc tcaaagcgga
      241 ttgaaacggg tgcacctaaa ggggcctttc gtccaggccc agctagtatc ttatacccct
      301 tgcacgcttt ttcttttacc cttgcccctg ttttgtgcta tgattttcag gcctttatca
      361 tttagaaccg attacattca caaggaggag atgtatggat tacgaactgc cggaaaacct
      421 tcgactgatg cgcgattcgg cccgccggtt tgtgaagaat gagctggaac ccatctccca
      481 aaaggtggag gaggaagaaa agataccgga gagcgtggtg caaaagatga gggagatggg
      541 ttttttcggc atgtcgatcc cggaagaata tgggggaatg gggctgggaa ctctgggcga
      601 atgcgttatg aatgaggaat tcgggcgagt caactccagt tttcgatcga ggtttggaac
      661 caacaatggc attggctgcc aggggatatt gatcgatggg accccggagc agaaagagaa
      721 atacctcccc cggattgcca gcggggagtg gacggcggcc ttcgccctca ccgaaccgaa
      781 cgccgggtct gatgcggcca acatccagac caaggccgag aaaaaggggg atgtatacct
      841 tctcaacggg ctgaagcatt tcatcaccaa cggggacatt gccgacgtag tcacggtcat
      901 ggcggtgacc gacaagacca aaggccctcg gggagtgacg gccttcatcg tggaaaaaac
      961 ctttcccgga tattctgtgg gaaagatcga caagaagatg ggaatccacg ggaacaatac
     1021 ctcggagatc gtgttcgagg actgccaggt gccggtaaag aatatcatag gactggaaga
     1081 aggacggggg ttcgtcacgg ctatgaaagt cctcgaaaaa gggagaataa ccatcgccgc
     1141 agtgtgcgtg gggaccgccc agtactgtct ggatacgagc gtcgcctatt ccaagcagcg
     1201 cgtgcagttc gggaaaccca tttgcaccaa ccaggccatt cagtggatgc tggccgatat
     1261 ggccatttcc atctatacgg caaggcagat cgtttacaac gccgcctggt gtcgcgatca
     1321 gaagcagagg gtgacccagc aggcggccat ggtcaaggtg tactgcacgg agatggtaaa
     1381 ccgggtggta gactcggccc tgcaaatcca cggaggcatg ggatacatga aagagtctcc
     1441 catcgagcga atatatcgtg atatgcgact gtacaggatt ttcgagggaa cctccgaagt
     1501 gcagcggatg gtcatcgcca gggagttgct gaaggactaa ggtaataggt aataggtccc
     1561 ggggccacag aggaaaaaag aatttattcc catgacctag ggactggaac ctcggactcg
     1621 ttttaacagg agggtttgaa tggctgggag aagaggctcc ggcaaagccg ggaatggacc
     1681 ggtaaccgtt cccatcgaca atacgggaca ggcttatctg gaagtcctgc gggaccgggg
     1741 aatcaaatat ttcttcggga atgcgggaac ggatttcgcc ccgctcatcg acgggtttgc
     1801 ccgcttggcg gcggagggaa aagaataccc gaaacccgtc ttggtccccc atgaatttgc
     1861 cgcggttagc atggcccacg ggtacgccat ggtcaccgga gaaccccagg tggtcatggt
     1921 ccatgtgaat gtggggacat cgaacgccct caccggggtg atgaacgcct cccgggccaa
     1981 catcccgttg attttcaccg ctgggaggac ccccattacc gaaggggaag tgcggggggg
     2041 acggaacctt cacatccact gggcccagga gtctttcgac caggcatcca tggtacgcga
     2101 gtacgtgaag tgggattacg agttgcgttg ctttgcccag gtggaaaaag tggtggaccg
     2161 ggcccttaac atgaccatgt ccgagcctaa agggccggtg tacctgagcc ttcccaggga
     2221 agtccttgcc gaagcccatg atgaatttac ctacacccct gccggacccc ggctctatgc
     2281 gaaacccggg ccggacgccg aggcgctccg ccaggctgct ctgctcctga agaatgccga
     2341 aaaccccctg gtgatcacct ccatggtggg aaagaatccg gctgccgtgg ggcagttggt
     2401 gaaactctgc gagtcctttg cccttccggt caccatcccc atgagcaacc tctatttgaa
     2461 ttttcccacc gaccatccca tgcacctggg attcgacccc atgaagtttg tgcccacggc
     2521 agacgtgata ctggtagtcg aagcggatgt tccctggttt ccgatccggg ccaagcccaa
     2581 accggaagcc aaagtcatcc agctgggaat cgatcctttc ttctcccgct atcccatgcg
     2641 ctcctttcca gcggatatcc ctctggcctg tgaccccgaa gccggcttaa aggctctgcg
     2701 ggaggccctg gaggctcaac caccacagag caaaagagac attcagaata ggtacgaaag
     2761 gctgtcccag gagcaccgca cccagagggc cggctggaag gcggcggcag agaggctgaa
     2821 gagtgaacgg cccatcgata tggaatatgt ttcctattgt gtggggcaag tgagagatgc
     2881 ggaaacgatc cttatcaatg agtatgactt gagggcccat caggtttccc tcaacgaacc
     2941 cggttctttt ttcagttctc ccatgtccag cggcctgggc tggtgcttcg gagcggcttt
     3001 gggagtgaaa ctggccaaac cggacactgt tcccatggtc tgcatgggcg atgggacgta
     3061 ccattttacc gctgcaacgg cctgccattt catggcccag aacctcccgg tggtgctggt
     3121 ggtcttcaat aaccaatgct ggaacgcagt caagggctcg gtcctcgacc tttatccgga
     3181 aggatgggcg gtgcagaaga aacattttgt cctctgcgat ctgaaacctg aacctgcata
     3241 cgagaagatc gtagaagcct tcggagggta tggagaaagg gtggaagacc ccgataagat
     3301 catccccgcc ctgaagcggg cggtgaaggc tgtcaggcag gagaaacgcc aggccctttt
     3361 gaatgtcatc tgcaagcatc cgtaatttcc tgaagatatc gatagacagg gaaaaagcgg
     3421 agaaaaagac gaagcaaaaa ggcccccagt gacgatctga gccattatct ctaatccgcg
     3481 tctgctacaa acggaaatga aatttctgat tttttccatg gttttttttc tcctgtgcgg
     3541 ggtgaccctg gaagcgggag tagaagggaa ggaaccaatg gctccgaaga tcgcgctccc
     3601 ctcagaggtg gcagggtgga aatgggatgg gaaggggaaa tcgttcaata ccaagactgt
     3661 cttcgagtat atcgacggag cggcggagct ctacctcgcc tacggtttcc ttgggttgaa
     3721 ggtctggcgc tatgaaaaga cgggggcacc ccccattaac ctggagattt atgagcaggg
     3781 ttcctctgaa gacgcctatg gcgttttttc ctttgagcag caggacgaac cggccggagt
     3841 cggccagggg tcggagtttg ggggagggct gctccgcttc tggaagggga gctatttcgt
     3901 cagcgtctat gcggaggggg aggggaaggg tgtggaagcg gccatccttg acatcggaaa
     3961 agctgcggcc caggccatcc ccataaccgg accagaaccg aagttggtcc agtttctgcc
     4021 gggtaaggaa ttcgggctgg tcgatagaag tatccggttt ctcaaaagcc acgtcctctt
     4081 aaaccagcgt ttttttatct cccaccaaaa cattttaaac ctcagccgta aaacagacgc
     4141 ggtattggcc gtgtatttgc ggggagggca gaaggcccac ctccttctta ttcgataccc
     4201 gagtgcagat gaagcgagaa tttcgctgga aagcttccgg aaggcctaca tgcccgatgc
     4261 cggcgagaag gaccgggtga agactgaaga ccggaaatgg accttcagcc gtcagcagaa
     4321 agattatctc ctccttgttt tcggcgcccc tacagaatcc gacgccgaat ccctattaaa
     4381 ggcaacagag gaaaaactga taactggcag cagataaacg aagatgttca gaattggaaa
     4441 atatcagata agaaggggct gaacacttta gttttttgaa aaagccgata tacttctctt
     4501 catctcccgt ttggtcaagg gatccgcaga aatctccccg ttcccccctt tgaaaaaggg
     4561 ggcggaggaa ggatttaaca tcgaaaaatg gaagaa
//
LOCUS       DASPRR010000622        16724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2090887, whole genome shotgun sequence.
ACCESSION   DASPRR010000622 DASPRR010000000
VERSION     DASPRR010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16724)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090887"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VEL68_22775"
     CDS             complement(<1..1061)
                     /locus_tag="VEL68_22775"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HXZ38871.1"
                     /translation="MNSIVGIKMMGYLIAAFLGATLFWPSESCAEEGKVSEFGKYQGY
                     SEAIYDGTQRISDYLTLSNGTRLAYDLILPTKSGVPADRPLPVLFKYTPYLRTYILYD
                     KNGKNMVAELYRWSEEEQEYVRKSSRVDPRGNLLDPLLRNRYLGKMVKYGYAVVVVER
                     PGTGASFGRTNMSFDALAKEANEVLNWIAAQSWCDGNIGMYGDSWQGQTQLAAASAGN
                     RHLKAIFPAGTWLDNYSSIIYPGGVYDKAFANFFAWTQKILSSEVITPVDRDRDGALL
                     AQARQERAQGTGPSFGDFTRMFAYRDSAIPNGMRPWIDAAAAYPFLDRINRAGIPIYL
                     VAGWYDFCARDMFLWYRNLT"
     gene            complement(1123..1800)
                     /locus_tag="VEL68_22780"
     CDS             complement(1123..1800)
                     /locus_tag="VEL68_22780"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HXZ38872.1"
                     /translation="MTNLISASSSNLDSLKRIGTGLSFILFPLLFILGFAFHPGLLSP
                     RLLLPADLVLRARHADLMQWGHVLDIIGAVLLVVIALRFMKLLNRGGSAAWAGFIGAV
                     LAIWGVIMLVALKGALCLTMSAIDSVPESEFAQMMPGLLAMFSRKGWMVLLWGIVCLP
                     IGFVMQAIPLVKTRAIPRWQGILFLIGVLLVGTPDGLEIVNLTACVLMALGLMPYGIQ
                     TLRNYSA"
     gene            complement(1845..2363)
                     /locus_tag="VEL68_22785"
     CDS             complement(1845..2363)
                     /locus_tag="VEL68_22785"
                     /inference="COORDINATES: protein motif:HMM:NF042671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HXZ38873.1"
                     /translation="MENFFEILRHTPWWVYLLFAYILYVGITAMKLRSVPVFQLFIVP
                     AIFTILSIHTLVGRIGDHFLYIIPWGIAAAIGIAIGWIEMKRLNIIVDRRNRLLKIPG
                     SAFTLILLLLFFGCKYYYGFMSATAPERAKEIQFVIFILLITGMGTGVMWVRGFGYFS
                     KYVKSHSVEVIK"
     gene            2629..4164
                     /locus_tag="VEL68_22790"
     CDS             2629..4164
                     /locus_tag="VEL68_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXZ38874.1"
                     /translation="MGFDENDFFRRATMQVLSSLDIEVAMWRALKFLREFIPADMMFL
                     HLYQRDLGAMRTIAMATPSEGKKIDRLHPLTKEVRTRVEERHPHVMILNEPELDPLGK
                     NMFQHFGTLDASGMVLHLDVEGERLGALSLVAMGRGKFSEEHARLMSLLNEPFAIAFS
                     HALQHQEVLKLKDLLADDNRYLHRELFHLSGDEIVGADFALKGVMAMARQVASLNSPV
                     LLLGETGVGKDVIANAIHYSSPRKDGPFVKVNCGAIPETLLDSELFGHEKGAFTGAIS
                     QKRGRFERADHGTIFLDEIGELPPQAQVRMLRVLQDKEIERVGGTISITLDIRLIAAT
                     NRNLEEMVKEKKFREDLWFRLNVFPIRIPPLRERKEDIPALVRHFVGRKSRELKLAAS
                     PSLARGAIDQLLAYHWPGNVRELENVVERALILSKGEPLAFDDLLGAKSGDRPPAAAT
                     PQGQSLNLDEVVSLHIQRVLEMTKGKVHGKGGAAEVLGINPSTLRNRMNQLGIPYGRR
                     ARA"
     gene            complement(4298..4564)
                     /locus_tag="VEL68_22795"
     CDS             complement(4298..4564)
                     /locus_tag="VEL68_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38875.1"
                     /translation="MRHIEVKYENGTHAFVDDYLLGALITSRQIQQFYRPSEKRWIRI
                     GVDPIRKEKVGYSGRERRRTGQYGEMLPLLPNPALGDSRTPGRI"
     gene            complement(4628..5314)
                     /gene="phoU"
                     /locus_tag="VEL68_22800"
     CDS             complement(4628..5314)
                     /gene="phoU"
                     /locus_tag="VEL68_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXZ38876.1"
                     /translation="MDSRFQHELDRLKELLLQMAGLAERAISNAIEALVKRDSSLAEK
                     TIAQDEKINHLEIEIDELCLELLALHQPLAADLRFIASAMRINTELERIGDQAVNIAE
                     RAISLNQEPQLKPYIDIPEMAEITQNMLRDVLNAFVNGDALLARSVCERDDRVDGLNN
                     QVFRELLTYMMADSKTITRAVHLLIVSRCLERIADHATNIAEGVIFMAQAKVIKHHAD
                     AAPERQEEKR"
     gene            complement(5392..6150)
                     /gene="pstB"
                     /locus_tag="VEL68_22805"
     CDS             complement(5392..6150)
                     /gene="pstB"
                     /locus_tag="VEL68_22805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXZ38877.1"
                     /translation="MESSVLRIENFSASYGEHSVLKEINLSFFRNTLTAVIGPSGCGK
                     TTLIRCINRMHELVPDARVSGKILLDNQDIYDTDPIIVRRQIGMVFQRPNPFPTMSIY
                     DNVIAGYNLNGIKLSRAERDHTVEESLKKAALWQEVKNSLHRRGTFLSGGQQQRLCIA
                     RALAMDPSVLLLDEPTSALDPKATAHIEELVSQLKNTITIIQVTHNIAQAARVSDFTA
                     FLYLGELVEFGPTARMFTGPKDRRTDEYLSGKFG"
     gene            complement(6132..6989)
                     /gene="pstA"
                     /locus_tag="VEL68_22810"
     CDS             complement(6132..6989)
                     /gene="pstA"
                     /locus_tag="VEL68_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXZ38878.1"
                     /translation="MNRMTEVSIHRRIWADKFFKVLIIVFSLGSIVPMFLILFMITRN
                     GISMISWEFLTQLPKPVGESGGGISNAIIGTLWLIVLSSLFSVPLGVFAGVFLAEYKG
                     GKLAHLVRSSVEILQGIPSIVIGIIAYVWVVMLMGSFSALSGGVALGIMMLPVIVTAT
                     EETLKLIPDTLREASLALGVSYPRTILKVILPAGLSGILSGILLGIARVAGETAPLLF
                     TAFGSPFMNQNILKPMSSLPLTIFNYATSPYPDWHTLAWGASFILIAFVLGLNLITKL
                     VTQRWKVQF"
     gene            complement(6986..7927)
                     /gene="pstC"
                     /locus_tag="VEL68_22815"
     CDS             complement(6986..7927)
                     /gene="pstC"
                     /locus_tag="VEL68_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXZ38879.1"
                     /translation="MNHTVADVGIPMARSQMTQRYKWGISEAAFRRFLYMAGLILLIL
                     LVAIFFSLVVASFPALREFGLSFLIGRTWDPVRGEFGGLVFLVGTLMSSLMALIISMF
                     FSLPASIFLGEYFRHGKVSAFMKSVIELLAGIPSVIYGFWGLFLLVPLVRVLEMKLGV
                     APQGVGIFTSSLVLAVMIVPYASSIGTEVISLVPSDLKEAALSLGATRFEVIRKVILP
                     YAYSGIFAGVLLALGRALGETMAVTMVIGNSNFLPKSIFSPGNTMASVIANEFTEATG
                     RLYLASLIELALVLFVVTTIINILGKLVIRKMTTRVT"
     gene            complement(8019..9062)
                     /gene="pstS"
                     /locus_tag="VEL68_22820"
     CDS             complement(8019..9062)
                     /gene="pstS"
                     /locus_tag="VEL68_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXZ38880.1"
                     /translation="MKMILRIFTLFSIFFGLCSLGGAKERELIGAGATFPYPLYSKMF
                     DVYHKETGVKVNYQAIGSGGGIRQLINKTVDFGGSDAIMSDKDLKEAGGPVLHIPTCA
                     GSVVITYNLTGEPRLKFTPDIIADIFLGKVKKWNDSRITAVNPEVKLPDTDITVVHRS
                     DGSGTTFIFSSYLSKVSQEWKEKVGSGPSLNWPVGLGGKGNPGVAGLVKQTPGSFGYV
                     ELIYALQNQMPYGTIKNKKGNFIEPTIASTSLAAETALPDDMKVDLTDTDAAKGYPLS
                     GFTWIIVYKEQDYAKRPEEEAKDLVDLLWWMTHKGQGYAEPLHYAPLSKVAVEKAEKL
                     IKSISYKGKPILK"
     gene            complement(9198..10373)
                     /locus_tag="VEL68_22825"
     CDS             complement(9198..10373)
                     /locus_tag="VEL68_22825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ38881.1"
                     /translation="MLGESISRPSKILITDDERDLVEMLALSLGRRGYEIARAYDGLE
                     AWDKVKEEKFDLLILDLMMPRLDGWEVCRFIRKSDDPQIRETPILILSARAMAEDRVK
                     GLEMGAEEYVTKPFSLMELMLRIEKLLNQRRAFASLKSDLAHLQRQTREQEERVCKIV
                     HDLKTPLVSMGASAKLLMKPREKDEALEMLRGIYENSHRLNRRLEEILLFSSSSFSER
                     KILMKEINLPLLTDRLRESFLALAREKMIEMKFYPLPSFPPLWGDERWLERALENLLG
                     NALKYTPEGGRVEVGGSVSPEEGMVEIWVRDNGRGIFPEEVQQIFHPFHRGRNALNEP
                     GMGLGLSLVKEVADLHGGKILVESKPQEGSTFSIVLPVGENGRKKGGERGAANFTNN"
     gene            complement(10767..12137)
                     /locus_tag="VEL68_22830"
     CDS             complement(10767..12137)
                     /locus_tag="VEL68_22830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ38882.1"
                     /translation="MNWKSPLQWKVSLIFAVLLAGGLWAVWEGKGPHGEFPGVLFLIV
                     LLAIVAILWFAEEKVLIQPLNEMTGVVEKFAEGLFNWRVRLGTRRDELGHLGNGLNRM
                     AQAVAEKVDRLAKNLAETEALLGGMEEGVLILDLNGRIKNINRSMGVILSQTYPSDVG
                     KHYLEVFRDPELNDLIQATLADKKGYRRSLAPLGQPGRTFQVQSSLVQYAENGAEGVI
                     VVFHDVTDLKRLERVRQDFVANVSHELRTPLTAIKGYVEALRDGGLQDPVQAEQFLEV
                     IQRHSERMDKIVSDLLLLSEMESADRVLQIETVHLPELIRTAVETLRPLAEGKKQNLR
                     IGPLEGLPRLRADGQKVHQVMINLLNNAICYTQEEGSITVEAKPVSEGVEVSVIDNGI
                     GIPPDDLPRIFERFYRVDKGRSRELGGTGLGLSIVKHIVEAHGGQVRVESKPGKGSRF
                     TFFLPR"
     gene            complement(12134..12829)
                     /locus_tag="VEL68_22835"
     CDS             complement(12134..12829)
                     /locus_tag="VEL68_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXZ38883.1"
                     /translation="MPKKILVIEDEKDIQELLQLYLKRDGYDVHIAKDGETGLRRASQ
                     ERYDLILLDLMLPQLDGLEVCRTLRSRPQTADIPIIMITAKAEESDRIVGLEIGADDY
                     ITKPFSPREVLARVKALFRRMEKPKTKEVRHEYGGIALDHSRHEVTYKGKSHSLTTKE
                     FNLLEYFLANKGRVLSRDILLNEIWGYDYFGTTRTVDVHVAHLRHKFQVLNKSLVAIK
                     GLGYKLQDEPVKT"
     gene            13221..15338
                     /locus_tag="VEL68_22840"
     CDS             13221..15338
                     /locus_tag="VEL68_22840"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXZ38884.1"
                     /translation="MGRMLWLAFLLGLCLAEGCVPGYKTPQDRSIPAKPAAARVLFFV
                     ADGMRPDLMESHARQGFMPAYSKLMDEGVRGLNGMIPPFPTNSGVCWYSLATGAYPAQ
                     HGSTNNTFHRIGEEDFNNRTSYSMPGVLQADTLASAAERAGKKVAQVHWVGGLNARIA
                     GPTVEFGNYFSNRGVLAAPFDSSEEASAAAFGVSYQNASLAPADGWINAPAGDPAAPP
                     KQAILIIPTKKPAENPDRVYNLYIYDSVVDGIPAYDRVILVPSACSKNGACKSADLAP
                     GDFKEVKLRGGEGLNGIQVGQTASFYVKLISLAPDLTTFRLYFTPVARVLATCSMDAC
                     NFLPPGAPGEDRLEKYIADNLPGFILSDPEPLQAGIIDEDTFVQQGLELEAIYGNAVM
                     SYILGTLQPNTDLALIGYPVPDEFQHQFLGLITPTDMDGTPNPCYDRARCQGALEDRL
                     AIRQGYIRNAYAGADAKLALAKRLMGPNPTVFAASDHGFAPHWYAVNAGKILEDAGLQ
                     STAQTANCRSGGAPTKAKACYSGATLQVYISLSGRDPGGLVPAAEYENVRNEIVSAFR
                     NLTDPENPGKQVVLKILKKEELRNVEGSDSLHPTRSGDVVVVLRPPYQFNASTPGKRI
                     APTPFFGGHGYLPNLVDIPHQVNMRCVFVAAGPGIRKQEPVPGIRAVDLAPTIASLMG
                     IPGPIDARGTILFNLSPAAGDPN"
     gene            complement(15687..16628)
                     /locus_tag="VEL68_22845"
     CDS             complement(15687..16628)
                     /locus_tag="VEL68_22845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38885.1"
                     /translation="MKKIALKEGFGIGILSAITLALLIVGLFAARVEAGPPGARIEYQ
                     VMTSDGLAVGYPFESWVVFDIPSNPAVPGIALPAGATFRFTFPSAFTPQSNFRPQAVL
                     LYGWSQGPIAVPFTVGLDPRDDRTIVLRLTNALPAGPPERPGLKAIHLRWGPLNPAQA
                     GDYPITVQLSDAGDFSGTTQAIAHIGSKPVANIAAYNTLHDGRNENWQRVKVGQPAAI
                     PIDFLVTLPDKARSFISLRPASEGSLDILSDGMPIGAISKSGVPLTLKPEPFGPGFAR
                     LGIIRLHATAGPEPGVAEIDAHLTGGTSYKITVLVER"
BASE COUNT         3850 a         4666 c         4414 g         3794 t
ORIGIN      
        1 gttaaattcc tgtaccagag gaacatatcg cgagcacaaa agtcatacca gccggctacg
       61 aggtaaatgg ggatccccgc ccggttgatt cggtccaaaa agggataggc cgccgcagcg
      121 tcgatccagg gcctcatccc gttcggaatg gcgctgtccc ggtaggcaaa catcctggta
      181 aaatccccaa aagatggccc cgttccctgg gcgcgctcct ggcgcgcctg ggccagcagg
      241 gccccatccc ggtcgcgatc cacgggagtg atcacctcgg aactcaggat tttttgggtc
      301 caggcgaaga agttggcgaa ggccttgtca tacacacccc ccgggtagat aattgagctg
      361 tagttgtcca gccaggttcc cgcggggaaa atggccttca ggtggcggtt gccagcgctg
      421 gcggcggcta gttgggtctg accctgccag gagtcgccgt acatgccgat gttgccgtcg
      481 caccacgatt gggccgcgat ccagttgagg acctcgttgg cctccttggc tagggcatcg
      541 aaggacatgt tcgttcggcc gaaggaggcg ccggttccgg ggcgttccac caccacaacc
      601 gcatagccat attttaccat ctttcccaga taccggttcc tgagcagggg gtccagcagg
      661 tttccccgcg ggtcaacgcg agagctcttc cggacgtatt cctgctcctc ctcggaccag
      721 cggtacagct cggcgaccat gtttttcccg ttcttgtcgt agaggatgta ggtgcggaga
      781 tagggcgtat atttaaacag gactggcagg ggccgatccg caggtacacc gcttttggtc
      841 gggaggatca gatcataagc cagacgcgtt ccgttggaga gggtcaggta atcggaaatc
      901 cgttgggtcc catcatagat cgcctcggaa tacccttgat acttgccgaa ttcggaaacc
      961 tttccctcct cggcacaact ttcagagggc cagaagagag tggctcctaa aaaggccgca
     1021 atcagatacc ccatcatctt tatacctact atcgagttca tttctcatct ccttgtttgt
     1081 tttttgagga tggcccttgc gagttcatgg gagaatacaa ccctaagcgc tgtaatttct
     1141 caaagtctgg atcccatacg gcattaaccc caaggccatt agaacgcaag cggtcagatt
     1201 gacaatttca agaccatcgg gagtccccac caggagcacg ccgattaaga acaatatgcc
     1261 ctgccagcga ggaatggcac gggtcttgac cagagggatg gcctgcatga cgaaaccgat
     1321 cggcaggcac acaatacccc acaacaagac catccaaccc tttctagaaa acatggccag
     1381 caggcccggc atcatctgcg cgaattcact ctccggcacc gaatcgatgg cgctcatggt
     1441 caggcataag gcacctttta aagccaccag catgataacc ccccagatcg ccagcacagc
     1501 gccgatgaat ccggcccagg cagcggaacc gccccggttt aacagtttca taaaccgcag
     1561 ggcgatcact acgaggagga cggcaccgat gatatccaag acatgtcccc attgcattag
     1621 gtctgcgtgg cgggctctca acaccagatc tgccggcaaa agcaggcggg ggcttaacaa
     1681 accaggatgg aaagcaaacc ctaatatgaa aagcaacggg aagagaataa aggaaaggcc
     1741 cgttccgatc cttttcaggc tatccagatt tgacgatgaa gcagagatta aatttgtcat
     1801 tttatttcct ttcgcccgtc gaggggtgcc gggttgccag ccttttattt gataacctca
     1861 acggaatgac ttttgacgta tttggaaaaa taaccaaacc cccggaccca cataaccccg
     1921 gtgcccattc cggtaatcag caggatgaaa ataacgaact ggatttcctt ggctcgctct
     1981 ggcgcagtgg cgctcatgaa accgtaatag tatttgcagc caaagaagag cagtaacagt
     2041 atcaaggtaa aggctgaacc cggtattttc aaaagacggt ttctccgatc gacgataatg
     2101 ttcaatctct tcatttcgat ccagccgatg gcaattccga tggcggcagc gatgccccat
     2161 gggatgatgt acagaaaatg atcgccgatt cggcccacga gggtatgaat cgagaggatg
     2221 gtaaaaatag ccgggacgat gaataactgg aacaccggca cgctgcgcag cttcattgct
     2281 gtgatcccta cgtacaggat ataggcgaag agaaggtaaa cccaccatgg cgtatgcctc
     2341 agaatctcga aaaaattttc catctttttc tcctttcgcg ataaagaagg ccgattaccg
     2401 attcaccttt tttttcttta tcagctctct ttgcaaaaca tcctattgct tcaaccgtgc
     2461 caagttggta aaccgaaaaa tattcaggag ttataaaatc tctccccctg cgattgccga
     2521 tattcgcaaa aacagattgc cttattcggc aattgagttc agaattgcgg tatatggcaa
     2581 tgtcctctat tcatctgcga gtcggttttc tttgcagggg gaaaagctat gggtttcgac
     2641 gaaaatgatt tcttccgccg ggcaaccatg caggtattaa gcagcctgga tatcgaagtg
     2701 gccatgtggc gcgcgttaaa atttctcagg gaattcattc ctgcggatat gatgttcctg
     2761 cacctgtacc agcgcgacct tggggccatg cggacgatcg ccatggccac gccctccgaa
     2821 ggaaaaaaga tagaccgtct ccatccgttg acgaaggaag tcaggacccg ggtcgaggaa
     2881 agacatcccc acgtcatgat tctgaatgag cccgagttgg atcccctcgg gaaaaacatg
     2941 tttcaacatt ttgggaccct ggatgcatca ggcatggttc tgcatttaga tgtcgagggg
     3001 gagagattag gggcgttgtc tctcgtggct atgggacgag gtaagttctc tgaggaacat
     3061 gcccggctca tgtcgctttt gaatgagcct ttcgctatcg ccttttccca cgctctgcag
     3121 caccaggaag tcctgaagct gaaagacctt ttggccgacg acaaccgcta cctgcaccgc
     3181 gagcttttcc atctctccgg ggacgaaatc gtcggcgcgg attttgccct gaagggggtc
     3241 atggctatgg cccgccaggt ggcttcgctg aatagtccgg ttttgctcct gggagagacg
     3301 ggagtgggca aagacgttat cgccaacgcc atccattatt cttccccccg caaggacgga
     3361 ccttttgtca aagtcaactg cggggcgatt ccggaaaccc tcctggacag cgagctcttc
     3421 ggccacgaga aaggggcctt taccggagcc atttcgcaaa aaaggggacg tttcgagcgg
     3481 gccgatcatg gaaccatctt tctcgatgag atcggggaac tcccgcctca agcccaggtc
     3541 agaatgctcc gagtcctcca ggacaaagag atcgagcgag tgggaggcac gatctccatc
     3601 accctggata tccggctcat agcggccacg aatcgcaacc tggaagagat ggtcaaggag
     3661 aaaaagttcc gagaggattt atggttccgc cttaacgtct tccccatacg catacctccg
     3721 ctccgagaaa ggaaggagga tattccggcc ctggtgcgac acttcgtggg acgaaaatcc
     3781 cgagaactga aactggccgc ctccccttcc ctggctcgtg gagccatcga ccaactgttg
     3841 gcctaccact ggccggggaa tgtgcgggaa ttggaaaacg tggtcgaaag ggcgttgatc
     3901 ttgagtaaag gagaaccgtt ggctttcgac gatctgctcg gggctaaatc cggcgaccgg
     3961 cccccggctg ccgctacgcc ccagggccaa tcgctgaatc tcgacgaggt cgtctcctta
     4021 cacattcagc gggtgctgga gatgacgaaa gggaaagttc acggtaaggg aggagccgcc
     4081 gaggtattgg ggatcaaccc cagcaccctg aggaatcgca tgaatcaact ggggataccc
     4141 tacgggcgaa gggcccgggc ataggagcga aggcaatccc caacccaagg ggcgccgtca
     4201 agattctgtc aattctgagg aagaatcttt ccagaggctt cccatcattc tcggaaaagc
     4261 cggattttgc cggatgagac ggaacgtaaa gggacggtta aatccgccct ggggtgcggg
     4321 aatcccccaa ggcgggattg ggtaacagag gaagcatttc cccgtactgg ccggtccttc
     4381 tcctctccct gccggaatac ccgacttttt ctttccggat cgggtcgacc ccgatcctaa
     4441 tccaccgctt ttccgagggc cggtaaaact gctggatttg tctcgaggtg atcagagcgc
     4501 ccagaagata gtcatccacg aaggcgtggg tcccgttctc atatttcact tcgatgtggc
     4561 gcatgatctt atcctttcca gctcgggaaa accgtctttt cccgaacgat gcaagttcac
     4621 ggggggatca tcttttttct tcctgtcttt cgggcgctgc atcggcatgg tgcttgatga
     4681 ccttggcctg ggccatgaaa atgaccccct cggcgatatt cgtggcgtgg tcggcgatcc
     4741 gttccaggca gcggctcacg atgagaaggt ggactgcccg ggtgatggtc ttggaatccg
     4801 ccatcatgta ggtcaggagt tcacggaaga cctggttgtt caacccgtcc acccgatcgt
     4861 cccgttcgca gaccgagcgg gcgagtaaag catccccgtt tacaaaggca ttcaacacgt
     4921 cccggagcat attctgggtg atttccgcca tttcggggat gtcgatgtag ggtttgagct
     4981 gaggttcctg attcaaagaa atggcccgtt cggcgatatt gaccgcctgg tccccgatcc
     5041 tttccagctc ggtattgatc cgcatcgccg aagcgatgaa ccggaggtcc gcagccaggg
     5101 gctgatgcag ggctaagagt tccaggcaca attcgtcgat ttcgatctcc aggtggttga
     5161 tcttttcgtc ttgggcaatg gtcttctccg ccaaactgct atccctcttg accagggctt
     5221 cgatggcatt actgatggcc cgctccgcca gtccggccat ctgcaagaga agctccttca
     5281 atcgatccaa ttcatgttga aatcggctgt ccatattatt ttccgcaagc cagcctcagt
     5341 ggtgcatcat aaaaatcaat tcccgatcca ctaggttctc taaaccttcg cttagccgaa
     5401 tttcccgcta agatattcat ctgttctcct gtccttgggc ccagtaaaca ttcttgctgt
     5461 gggtccaaac tcaacgagct cccccagata aagaaaggcg gtaaaatcgg aaacccgggc
     5521 ggcctgagca atgttgtgcg taacctgaat gatggtgatc gtattcttca actgggaaac
     5581 cagctcttcg atgtgggccg tggctttggg gtccaaggcc gaggtgggct cgtcgaggag
     5641 gaggaccgac gggtccatgg ccaaagcccg ggcaatgcaa agcctctgct gctgtccgcc
     5701 cgaaaggaat gtccccctcc ggtggagcga gtttttgact tcttgccaca gagcagcttt
     5761 tttcagcgac tcctcgaccg tatgatcccg ttcggccctg ctcaatttaa tcccgttaag
     5821 attgtatccg gcgatcacat tgtcatagat gctcatggtt gggaagggat tgggcctctg
     5881 aaaaaccatt ccgatctggc gacgtacaat aatgggatcg gtgtcataga tatcctggtt
     5941 atcgaggagg attttgcctg agacccgggc atcggggacc aactcgtgca tccgattgat
     6001 gcaacggatg agggttgttt tcccgcatcc cgagggtcca atgaccgcgg tcagggtgtt
     6061 ccggaaaaag gaaagattga tctccttcaa gacgctatgt tcgccgtacg aggcgctgaa
     6121 attctcaatc ctcagaactg aactttccat ctttgggtca ccagtttggt tatgagattc
     6181 aggcccagca caaaggcgat caggatgaaa gaagcccccc aggcaagggt atgccaatca
     6241 ggataagggc ttgtcgcata gttgaagatc gtcaggggca ggctgctcat gggttttaaa
     6301 atattctggt tcatgaaagg gctgccgaag gcggtaaaga gaaggggcgc cgtctccccg
     6361 gccacgcggg caatacccag gagaatcccg gaaaggattc cgctcaatcc cgccggcagg
     6421 attaccttta aaatcgtccg gggataagaa acccccaggg ccagcgaggc ctcccgcagg
     6481 gtgtcgggaa taagttttaa ggtttcttcc gtggccgtga caatcaccgg aagcatcatg
     6541 atccccaggg ctaccccccc ggaaagagcc gagaatgacc ccatcagcat caccacccac
     6601 acgtaggcga tgatcccgat cacgatggag gggatcccct gcaggatttc aaccgatgag
     6661 cggaccaaat gggccaattt tccaccctta tattccgcaa gaaaaacccc cgcgaaaact
     6721 cccagaggaa cagagaaaag agaggagaga acgatgagcc agagagtccc aataatcgcg
     6781 ttcgagatgc ccccgccgct ctccccgacg ggtttcggaa gctgggttag aaattcccag
     6841 ctgatcatgg aaatcccgtt ccgggtgatc atgaagagga tcaaaaacat gggcacaatc
     6901 gagcccagcg aaaacacgat gatgaggact ttgaagaatt tgtcggccca gatccggcgg
     6961 tgaatcgaga cctcagtcat cctgttcatg ttaccctcgt agtcattttg cgaatgacca
     7021 gtttccccag gatattgatg atcgtggtaa cgacgaaaag gaccagggct agttcgatca
     7081 aactcgctag gtacaggcga ccggtcgctt ccgtgaattc gttggcgatg acgctggcca
     7141 tcgtattgcc gggactgaag atgctcttgg gaagaaaatt cgaattcccg atgaccatgg
     7201 tcacagccat cgtttctccc agggctcttc cgagggccag aagaactcct gcgaaaatgc
     7261 ctgaatacgc gtatggcagg atgaccttgc ggattacttc gaaccgagtg gcccccaatg
     7321 aaagggccgc ttctttcagg tcggagggga ccagactaat cacttccgtg ccaatggatg
     7381 aagcataggg aacaatcatg accgccagga ccagcgagga ggtgaaaatt ccgaccccct
     7441 gaggcgccac ccccagtttc atctccaaga ctcgtaccag gggaaccaga aggaagagtc
     7501 cccagaaccc gtagatgacc gaagggattc ccgcgagtaa ttcgatcacg cttttcatga
     7561 aggcggaaac ctttccatgc cggaaatact cccccaagaa aatggaagcc ggtagcgaaa
     7621 agaacatgga aatgatcaaa gccataagag aactcatcag cgttccgacg agaaagacga
     7681 ggccgccaaa ctctccccgt acggggtccc aggtccggcc gatcaggaag ctgaggccga
     7741 attcacgcag ggcaggaaag gaggccacta ccaacgaaaa aaaaatggcc accaggagaa
     7801 tcaacaaaat aaggccagcc atataaagga atctgcggaa ggcagcttcc gaaatacccc
     7861 atttgtagcg ctgggtcatt tgggagcggg ccatgggaat ccccacatcc gcaaccgtat
     7921 gattcatttt tctctccatg ccataattat attccccctg cagagcagga atgagaataa
     7981 ggggcagagc gggaggaaat tcgcgcccca cccctttttt acttcaggat cggttttcct
     8041 ttgtagctaa tggacttaat cagtttctct gccttttcta cagccacctt ggagaggggg
     8101 gcataatgta aaggttcagc atatccttgt cccttgtggg tcatccacca gaggaggtcc
     8161 acaaggtcct tagcctcttc ttcaggcctt ttggcatagt cttgttcctt atagactata
     8221 atccaggtga aaccactgag gggatagcct ttggcagcgt ccgtatcggt caaatccact
     8281 ttcatgtcat ccggcaaggc agtctctgct gccaggcttg tcgatgcgat cgtgggctct
     8341 ataaaatttc ccttcttgtt cttgatcgtc ccatagggca tctggttctg gagggcatag
     8401 atgagctcca catacccgaa gcttccaggg gtctgtttca ccagaccggc gactccgggg
     8461 ttccctttgc cccctaaccc cacgggccag ttcaatgaag gaccggaacc cactttttcc
     8521 ttccattcct ggctgacctt gctcaaatag ctgctgaaga taaaagtcgt accgctgcca
     8581 tcggatcggt gaaccaccgt aatgtcggta tcgggaagtt tcacttcagg attgaccgcc
     8641 gtgatccgcg agtcattcca ttttttgacc tttccgagga agatatcggc aataatatcc
     8701 ggcgtaaact tgagccgggg ttcgccagtc aaattatagg tgatcaccac tgagccagcg
     8761 caggtgggaa tatggagaac gggaccgcca gcctccttta aatccttgtc gctcatgatc
     8821 gcatcggacc cgccaaaatc cactgttttg ttgatcagtt gccgaatccc cccgccagag
     8881 ccgatcgcct gatagttaac ctttactccc gtttctttat ggtaaacatc gaacatcttg
     8941 gaatacagag gataggggaa agtagccccg gcgcctatga gttccctttc tttggcccct
     9001 cctaaactgc aaaggccaaa gaaaatggag aataacgtga agattcttaa tatcattttc
     9061 atcctaaacc ccctaaaaag aattttttcg cggcttttca gctccctttt cattaatact
     9121 cctccctttg gaattagaaa tcgaacttgt tcttatttaa aatagtattc ctctgttagc
     9181 agtctgttag ccgaatgtta attatttgta aaatttgcgg cccccctctc acctcctttt
     9241 tttcgcccat tttctccaac gggcaaaacg attgaaaatg tactcccctc ttgcggcttg
     9301 ctttccacca gaattttgcc accgtggagg tctgccactt cctttaccaa ggaaagtccc
     9361 agacccatcc ccggctcatt cagggcattt ctcccccgat gaaaggggtg gaagatctgt
     9421 tgaacctcct cggggaaaat gccccttccg ttgtcccgaa cccatatttc caccatcccc
     9481 tcttccgggc tcacggaccc ccccacctcc acccggcctc cctccggggt gtacttcagg
     9541 gcgttgccaa ggaggttttc caacgcccgt tcaagccacc gctcatcccc ccagagaggg
     9601 ggaaaagagg gaagaggata aaacttcatt tcaatcattt tctcccgggc cagggccaaa
     9661 aaagattctc tcagcctgtc cgtcaggagg ggaaggttga tctccttcat cagaattttt
     9721 cgttcggaaa atgaggatga ggaaaacaga aggatttctt ccagcctccg gttcagccgg
     9781 tgactgttct catagatccc gcgcagcatt tccagcgctt cgtctttctc cctcggtttc
     9841 atcaagagtt tggccgaagc tcccatggaa accagggggg tcttcagatc gtgaacgatt
     9901 ttgcagaccc tttcttcctg ctcacgggtc tgcctctgca gatgggccag gtccgacttc
     9961 agggaagcaa aggcccggcg ttgattcagg agtttttcga ttcgcaacat cagttccatg
    10021 agggagaaag gcttggtgac gtattcttct gcccccattt ccaacccctt cacccggtct
    10081 tcggccatgg cccgggcgct cagaatgagg atgggggtct ctcggatttg cgggtcgtcg
    10141 ctcttgcgga tgaaccggca gacttcccac ccgtccaagc ggggcatcat caggtccagg
    10201 atcaggaggt cgaacttctc ttccttcacc ttgtcccatg cttccaggcc atcataggcg
    10261 cgggcgattt catatcccct ccggcccagg gataaggcca gcatctccac caagtctctc
    10321 tcatcatctg tgatcaggat tttagaaggc cgggagatac tttctccaag catctggttc
    10381 ccctcctttt ttcatgttcc tccttgaagg aggggcgtag catcggggcg agcgttcata
    10441 agccggaggg aataaaagaa gggggcaccg gctgaaatct ccggagaaac aatccgtctc
    10501 cattccctcc gggttcaatt ccattcccct tttccccact gccctgcata gagcgattcg
    10561 tcctctttct aggtacgggc aactcatcgg gaactccctt cagatgtttg gctgtatttt
    10621 acgggctctt gattaagcca gcataaaggc agggtaaaat ttctgtaaat tcagccagtc
    10681 gatggaaggg cttttcctga agaaggagtt tggagactgg agatgttcct taaggaaccg
    10741 gcgcaagtga tggatggggt gatttttcag cggggaagaa aaaaggtgaa gcgggaccct
    10801 ttgccgggtt tgctttctac gcgaacttgt ccgccgtggg cttcgacgat gtgcttgacg
    10861 atggaaaggc caaggcccgt cccccccagc tcgcgggatc gccctttgtc cacccggtag
    10921 aaccgctcga aaattcgagg cagatcatcg ggcggaattc cgattccatt gtcgatgacc
    10981 gagacctcta cgccttcgga gaccggcttg gcctctacgg ttatcgagcc ttcttcctgg
    11041 gtgtagcaga tcgcgttatt caggaggttg atcattacct gatggacctt ctggccatcg
    11101 gcccgcagtc tggggagccc ctccagaggc ccaatccgaa ggttctgctt tttcccctcc
    11161 gccaggggcc gtaaggtttc cacggcggtc cggatgagct ccggaagatg gaccgtttcg
    11221 atctgcagaa cccggtctgc cgactccatt tccgacagga gcagaaggtc ggagacgatt
    11281 ttgtccattc gctcggaatg gcgctgaatc acctccagga actgttccgc ctgaacggga
    11341 tcctgcaatc ctccgtcgcg caacgcctcc acgtaccctt tgatcgccgt gaggggggtg
    11401 cgaagttcat gggacacgtt ggccacaaaa tcctggcgta cccgctccag tcttttcagg
    11461 tcggtcacgt cgtgaaaaac cacgatgacc ccctcggctc cattttccgc atactggacc
    11521 agggaactct gcacctgaaa ggtcctgccc ggctggccca ggggagcaag acttcttcga
    11581 tatccctttt tgtcggccag ggtggcctgg atgaggtcat tcagttccgg gtcccggaag
    11641 acttccagat aatgcttccc cacgtccgaa gggtaagttt gggacagaat cactcccatg
    11701 gatcgattga tattcttgat ccgtccgttt aggtccagga tgaggactcc ctcctccatt
    11761 ccccccagga gagcttcggt ttcggctagg ttcttggcca accgatccac tttttccgcc
    11821 acggcctggg ccatccggtt cagcccattc cctaagtggc ccaattcatc ccgccgggtt
    11881 cccaaacgca ccctccagtt gaaaagaccc tccgcgaatt tttcaacgac cccggtcatt
    11941 tcgttcaatg gctggatcaa aaccttttcc tctgcgaacc aaaggatggc cacgatggcc
    12001 agaagaacaa tcaaaaaaag gaccccgggg aattccccat gaggcccctt cccttcccag
    12061 acggcccata ggccacccgc caggagaacc gcaaagatga gggagacttt ccactgcagc
    12121 gggcttttcc agttcatgtc ttgaccggtt cgtcctgcag cttgtacccc agacctttaa
    12181 tggccaccag ggacttgttc aggacctgaa atttgtgccg cagatgggcc acatggacat
    12241 ccaccgtccg ggtcgttcca aaataatcgt acccccagat ctcatttaag aggatatccc
    12301 gggatagaac ccgtcccttg ttggccagaa aatactccaa tagattaaat tcctttgtgg
    12361 tcaaggaatg ggattttcct ttgtaggtga cttcgtgcct ggagtgatcc agggcgatcc
    12421 ctccatattc gtgcctgacc tctttggttt tgggtttttc catccggcgg aagagcgcct
    12481 tgacccgggc cagaacctcc cgggggctga aaggcttggt gatgtagtcg tcggctccga
    12541 tctctaggcc cacgatgcgg tcagactctt ccgccttggc ggtgatcatg atgatgggaa
    12601 tgtcggcggt ctggggacgg gagcggaggg tccggcatac ttccaggccg tccagctggg
    12661 gcagcatcag gtccagcagg atcaggtcgt aacgctcctg ggaagctctt cgcagtcctg
    12721 tctccccatc cttggcaatg tggacgtcgt atccgtcccg cttcaaataa agctgcagga
    12781 gctcctggat gtccttttca tcttcgatga ctaggatttt cttgggcatc ggctcatcct
    12841 atcacagacc gcctcggagg atcaacctca cccggggtcc tgcagtccaa tccctggaaa
    12901 ggaatagagg gggctcagaa agataagcat ttccgtcaca aattcacagt aataccggga
    12961 ggatgttcta tcggagacga gctgttcgca ttaatttaag gatacacctt tgaacttccg
    13021 gatggatgaa agatcggtta caatggttta attttcggct ttgagccctc ctccgggtta
    13081 cgggtttaat cgtcgggcgg aaaagcgatg ataccgacgc gatccagggc ttctgaaatc
    13141 aacgcggggt atataggatg attttgaggc aggaaaagaa tattttcccg actacgtcct
    13201 gaaggcgaag gagaatgaac atgggaagga tgttgtggct tgctttcctc cttgggttat
    13261 gcctcgctga agggtgcgtt cccgggtata aaactccaca ggatcgttcg atacccgcaa
    13321 aaccggcagc agccagggtt ctgttctttg tcgccgacgg catgcggccc gacctgatgg
    13381 agagccatgc ccggcagggc ttcatgcccg cctattcgaa gctcatggac gaaggcgtcc
    13441 gaggcctcaa cggaatgatt ccacccttcc ccaccaattc cggggtctgc tggtactccc
    13501 tcgccaccgg tgcctacccc gcccagcacg ggtcgacaaa caatactttc caccgaatcg
    13561 gcgaagaaga tttcaataac cgcacttcat actccatgcc cggagtcctc caggccgaca
    13621 ccctggcctc ggctgcggag agagccggca agaaggttgc tcaggttcat tgggtgggcg
    13681 ggctgaacgc caggattgca gggcccaccg tcgaattcgg aaattatttt tccaaccggg
    13741 gcgtgcttgc tgccccattc gattcttcgg aagaggccag cgccgccgcc ttcggggttt
    13801 cctatcagaa tgcctccctg gctcctgcag atggatggat caatgctcca gcgggcgacc
    13861 ctgccgctcc ccctaaacaa gctattctca tcattccaac caaaaaaccg gcagagaatc
    13921 ccgaccgggt ctacaacctg tatatttacg acagcgtggt ggacggcatc ccggcctacg
    13981 atcgggtcat tcttgtcccc agcgcctgct ccaaaaatgg ggcttgcaaa tcagccgatc
    14041 tggccccggg ggatttcaaa gaagtcaagc tccgcggcgg ggaaggctta aacgggatcc
    14101 aggtcgggca aaccgctagc ttctatgtca agctcatctc cctggcccct gatctaacca
    14161 cattcaggct ctatttcacc cccgtggccc gcgtattagc cacttgcagc atggatgctt
    14221 gcaatttcct tcctccggga gcgcctgggg aagatcggct ggagaaatac atcgccgaca
    14281 acctgcccgg tttcatcctg tccgacccgg agccgctgca ggcggggatc atcgatgaag
    14341 atacctttgt ccagcagggg cttgaattgg aagctattta cggaaatgcg gtgatgagct
    14401 atatccttgg gactctccag cccaatacgg acctcgcctt gataggctat ccggtccccg
    14461 atgagtttca acatcaattc ctgggactca ttactcccac ggatatggat gggacgccca
    14521 acccctgcta cgaccgggcg cgatgccaag gggccctgga agatcgtcta gccattcgcc
    14581 agggctacat ccgcaatgcc tatgccggtg ccgacgccaa gctggctttg gccaagcggc
    14641 tgatggggcc caatcccacg gtcttcgccg catcggacca cggctttgcc cctcattggt
    14701 acgcggtcaa cgcaggcaaa atcctggagg atgctgggct gcagtccact gcgcagaccg
    14761 ccaactgccg gtcaggagga gcgcccacca aggccaaggc ctgctattcg ggcgccaccc
    14821 tacaggtcta catcagcctc agcgggcgcg atccaggcgg tctggttccc gccgccgaat
    14881 atgaaaacgt ccgcaacgag attgtttccg ctttccggaa cttgacggac ccggagaatc
    14941 cgggcaagca ggtggtcttg aagattctaa aaaaagagga gctgcgcaac gtggagggta
    15001 gcgattcgct ccacccaacc cgcagcggag atgtggttgt agtgctcagg ccgccctacc
    15061 agtttaatgc ctctaccccc ggcaaaagga ttgctcccac gcccttcttt ggcgggcatg
    15121 ggtaccttcc gaatctggtt gacatccctc atcaggttaa tatgcgctgt gtgttcgtgg
    15181 cggcaggccc ggggatccgc aagcaggagc cggtcccggg catccgggcc gtcgacctgg
    15241 ccccgactat cgccagcctg atgggcatcc cgggcccgat cgacgcccgg gggacaatcc
    15301 tcttcaacct ctcccccgct gctggggacc cgaattagcc accataagaa tctgcctccc
    15361 aagatttgaa agactttgcc ccccttggtt atctctttcc ccctacaagc actcgggcga
    15421 gggtaccttt aataattgct tattaaaggt ggaaatggcg gaacctgatt caaacaaatg
    15481 atcccgttat tgaaactttt ctgatgattg gtaataaggt agaaagaggc ctcgaaactt
    15541 cttgtctcac gccatcaccg caatatatag ttccccaaga aacaaggtct ctctaagtga
    15601 ttgttgctga atttcctcga aataataacc acttttccgg gggatggata attctaccat
    15661 ccccctctta cggcaaacgg gatctttcat cgttcgacaa gaaccgtaat tttataactg
    15721 gttcccccgg tgaggtgggc atcgatttcc gcgactccag gttccgggcc ggctgtggcg
    15781 tgaagtcgta taatccctaa gcgggcgaaa cccgggccaa aaggctccgg ctttagcgtg
    15841 aggggaacac cgcttttcga aatggcgccg atgggcattc cgtcgcttag gatatcaaga
    15901 ctcccttcgc tggccggtcg gagggaaatg aaagaacggg ctttatcggg taacgtgacc
    15961 aaaaaatcaa tgggaatagc cgcaggctgc cctaccttca cgcgctgcca attctcattc
    16021 cgcccatcgt ggagggtgtt ataggccgca atgttcgcta ccggcttgga gccaatgtga
    16081 gcgatggctt gcgtagtccc tgagaaatcc cccgcatccg acaattgaac cgttatcgga
    16141 tagtcgccag cttgagcggg attaagaggc ccccagcgca agtggattgc tttcaggcca
    16201 ggacgctcag gtggaccggc cggtaaggcg ttcgtcaacc tcaatacgat tgttcggtca
    16261 tcccgagggt ccaacccgac ggtgaaaggc accgcgatgg gaccttgaga ccagccatac
    16321 aggaggactg cctgagggcg aaaattggac tgaggtgtga aggccgaagg gaatgtgaag
    16381 cggaacgtgg ccccagcggg cagggcaata cccggtacgg cgggattgga aggaatatca
    16441 aaaacaaccc aggattcaaa cgggtagcca accgcgagtc catcgcttgt catgacctgg
    16501 tattcgattc ttgccccggg cgggcctgcc tcaacacgcg ctgcaaacag ccctacgatt
    16561 agcagagcaa gtgtgatcgc ggaaagaatg ccaattccaa aaccttcttt taatgcgatt
    16621 tttttcattt ttaccccctt caaggatatt accgttaatt aagatgcaat attggcccgc
    16681 tggattctct aaattcatga cttctcttaa gaaaattcta ttac
//
LOCUS       DASPRR010000623         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2403800, whole genome shotgun sequence.
ACCESSION   DASPRR010000623 DASPRR010000000
VERSION     DASPRR010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403800"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VEL68_22850"
     CDS             <1..1740
                     /locus_tag="VEL68_22850"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXZ38886.1"
                     /translation="IGGTGLATKILYDETGPETDPLGPKNRLIFMTGPFAATPVITSG
                     RHHMVTKSPLTGAYTESDTGGTWGPFLKRAGFDGVVVTGKARKPVYLWVTEGKAEIRD
                     ASRFWGMDTYVLDEAIRKDTHSEAVVASIGPAGEKGVRYASILNDGKEARAAGRGGTG
                     AVMGSKNLKAIAVYGKMQVEIADAEGLRASLKEISPMVAKNTEGMRKNGTAGGLATFE
                     ALGSLPLQNWKYQGRWEQGAAKIAGPAMTEKILTGNYFCEKCVIGCGRRVKVDKGPFA
                     PVEGAGPEYESVAMLGSLCLVDDIEAIAKANEVCNRYGMDTISAGAAIAFAMEAYEKG
                     IITKKDTGELELLWGRGDVMVKMAEKIGKREGLGQLLGEGVRIAAEKLGKNAVEFSIH
                     VKGQEMPAHDPRCFNAGAVGYATINRGACHLGFSHVFERILSMPEIGIDQPLPRLEVK
                     GKGEAAAKTQNIMGLFDSLKVCKFSMFGGLKMTPILAWYNMVTGNSMTMEEFLKTGER
                     IFNLKRMYNVRCGISRKDDTLPARFLTLKHEGEGLNPSLPPLGEMLYDYYKFRGWNEE
                     GIPNPEKLKELGL"
     gene            2028..2915
                     /locus_tag="VEL68_22855"
     CDS             2028..2915
                     /locus_tag="VEL68_22855"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HXZ38887.1"
                     /translation="MLASRKRRSRKKGRRGRKYLYLLVSVGVIGSLALFFLLRTKPVE
                     KKVIQPMPPVPEPAAKLPEEGKKEKEPPSKPAPRLALIIDDGGYNLDKVKALMEIGRP
                     FTLSVLPNTPFAQKVALLAHQGGAEVMLHLPMEPKENDRYSLEPDTVLSGMEKKEIQA
                     ILQRDLKGVPHVRGVNNHMGSKATEDPQVMKALMEFLKKEGLYYVDSHTSAHSVGPQI
                     ARQAGVAFGSNDRFIDQEKDLEVIKKAIRLAMKKAKKEGKAIAIGHPHSLTARAIREM
                     VPEIEEAGIKLVVASEVVG"
     gene            2957..>3408
                     /locus_tag="VEL68_22860"
     CDS             2957..>3408
                     /locus_tag="VEL68_22860"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HXZ38888.1"
                     /translation="MAWVGEALAVPRLLSVRKSSSPESTRVVLDLEAPPDYEVQSSAA
                     SPILTIYLQGLNLPQGAQEILIEDRVVRKVRIDPGDREGIKVILFLYQPTRSNVFVLK
                     PEGNKPDRLVIDVFRPAGEMRETPAKPEVPPSKEESREAKSAPAKAEKP"
BASE COUNT          837 a          884 c         1021 g          666 t
ORIGIN      
        1 atcggcggaa ccggcctggc caccaagatc ctctatgatg aaaccggccc agaaaccgat
       61 ccgctgggac caaaaaatcg gctgatcttc atgaccggac ccttcgctgc caccccagtc
      121 attacctccg ggcggcatca catggtcacc aaatcacctc tgaccggagc ctataccgag
      181 tctgataccg gcggaacctg ggggcccttc ctgaagagag ccgggtttga cggggtggtg
      241 gtcacgggca aggcccgcaa acccgtttac ctatgggtaa ccgaaggaaa agcggaaatc
      301 cgcgatgctt cccggttctg gggaatggac acctatgtac tggatgaggc catccgcaag
      361 gatacccact cggaggctgt cgtggcttcg attggtccgg ctggagaaaa gggcgtgcgg
      421 tatgccagca tcctgaacga cggcaaagag gcgcgggccg ccggtagggg ggggaccgga
      481 gccgtcatgg ggtccaaaaa cctgaaagcg attgccgtct atggcaagat gcaggtggag
      541 atagccgacg ccgaagggct gagagcctcc ctcaaagaaa tcagccctat ggtggccaag
      601 aataccgagg gaatgcgcaa aaatgggaca gcgggcggtc ttgccacttt tgaagccctg
      661 ggaagtcttc ccctgcagaa ctggaaatat cagggccggt gggaacaggg ggcggccaaa
      721 atcgccggcc cggccatgac cgagaagatc ctaaccggca actacttttg cgagaaatgc
      781 gtcatcgggt gcggccggag ggtcaaggtg gacaaggggc ccttcgcccc agtggaaggc
      841 gcaggaccgg aatacgagtc cgtagccatg ctgggcagcc tctgcctggt ggatgacatc
      901 gaagccatcg ccaaggccaa cgaggtctgc aatcgatacg gaatggacac catctctgcg
      961 ggggcggcca tcgcctttgc catggaggcc tatgagaagg gaatcatcac caagaaagat
     1021 acgggggaat tggagcttct ctggggccgg ggggatgtga tggtcaagat ggccgagaaa
     1081 atcggcaaac gggaagggtt gggacagctt ctgggggaag gggtgcgcat tgccgccgaa
     1141 aagctgggca aaaatgccgt tgagttctcc atccatgtga aggggcaaga aatgcccgcc
     1201 cacgatcccc gctgcttcaa cgccggagcg gtggggtatg cgaccatcaa ccggggggcc
     1261 tgccacctgg gattcagcca tgtctttgag cgcatactga gcatgcccga aatcgggatc
     1321 gatcagcctc tgccgaggct ggaggtgaaa gggaagggcg aagcggcggc caagacgcag
     1381 aacatcatgg gcctcttcga ttcgctcaag gtctgcaagt ttagcatgtt cggcggcctc
     1441 aagatgactc cgattttagc ctggtacaat atggtaacgg gtaactccat gaccatggaa
     1501 gagttcctga agaccgggga gaggattttc aatctgaagc ggatgtacaa tgtccgctgc
     1561 gggatcagcc gcaaggacga caccctgccg gcccgtttcc tcaccctgaa acacgagggc
     1621 gaaggtctga atccgagtct cccaccgctg ggagagatgc tctacgacta ctacaaattc
     1681 cggggatgga atgaagaagg aatccccaat ccggaaaagc tgaaggaatt gggattgtaa
     1741 aaaaaatgcg gatttcagat tgcggatttc ggatttagaa attcgcaaag gaaaaggata
     1801 gttctctctt tacgggggcg agccgtcacg tacttcgggt gatcgccctt catcctggat
     1861 taaggcgggt cttcttaaca agcccgcctt tttttctttc cgcaactttt caataggtct
     1921 attcggcaag tttcctttgg gttggttttt caatccgcaa tccgcaatgg gcaggttgat
     1981 ctattccatt gagaaaattc atccggttgg atagaataag tttcaaaatg cttgcatcga
     2041 ggaagagaag aagcaggaaa aagggaagga gaggcagaaa atacctttac cttcttgtct
     2101 ctgtcggagt gattggctcc ctggctctct ttttcttgct gcggacgaaa ccggttgaaa
     2161 agaaagtcat tcaacctatg ccccctgttc ccgagcctgc ggccaagctg ccggaagagg
     2221 ggaaaaagga gaaagaaccg ccctcgaaac ccgctccccg gctggccctg atcatagacg
     2281 atgggggcta taatttagat aaagtcaaag ctttgatgga gatcgggcgg ccttttacct
     2341 tgtctgtctt gcccaacacc cctttcgctc aaaaagttgc cctcctggcc caccaggggg
     2401 gagctgaagt tatgctccat cttcccatgg agcccaagga aaacgaccgc tattctctgg
     2461 agccggacac ggtcctgagc gggatggaga aaaaagaaat tcaagctatt ttgcagcggg
     2521 acttgaaggg ggttccccat gtccgggggg tcaacaacca catggggtcc aaggccactg
     2581 aggaccccca agtgatgaag gcattaatgg agttccttaa aaaggagggc ctttattatg
     2641 tggacagcca caccagcgcc cacagtgtag ggccgcaaat cgcgcgccaa gctggggtgg
     2701 ccttcggaag caacgaccgg tttatcgatc aagaaaaaga cctggaggtc atcaaaaaag
     2761 ccatccggtt ggccatgaag aaagcgaaaa aagaggggaa agccatcgcc ataggccacc
     2821 cccattccct gaccgctcgg gccatccgcg agatggtccc ggaaatcgaa gaagcaggaa
     2881 tcaaattggt cgttgcttct gaggtggtag gatgagaagg gttcttctcc tcactgcggt
     2941 tttaggaatt tggaccatgg cttgggtcgg ggaggctctc gctgtacccc gcctgttgag
     3001 tgttcgcaag tcgtcctccc cggaatcgac tcgagtggtt ctggacctgg aggcccctcc
     3061 ggactacgaa gttcaatctt cggccgcgtc tcccattctg acgatctatc tgcaaggact
     3121 gaatctgccc caaggagccc aggagattct gatcgaggat cgggtggttc gaaaggtaag
     3181 gatcgatccg ggcgaccggg agggcatcaa ggtaattctt tttctctatc agcccacgcg
     3241 ttcaaacgtc ttcgtcctga aaccggaggg aaacaagccg gaccgcttgg tgatcgatgt
     3301 tttccgcccc gcgggagaaa tgcgagaaac acctgcgaag ccagaggtcc caccctccaa
     3361 ggaagaatcc cgggaagcga agagcgcgcc tgccaaggcg gaaaagcc
//
LOCUS       DASPRR010000624         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1439761, whole genome shotgun sequence.
ACCESSION   DASPRR010000624 DASPRR010000000
VERSION     DASPRR010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439761"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VEL68_22865"
     CDS             complement(<1..210)
                     /locus_tag="VEL68_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ38889.1"
                     /translation="MDFLEIPYPAHICDLTWKLLSSGAFKIDKSANDDFRVTYHDPCN
                     VARAAGYFEPPRNILRAVCNHFTEMH"
     gene            complement(262..1155)
                     /locus_tag="VEL68_22870"
     CDS             complement(262..1155)
                     /locus_tag="VEL68_22870"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXZ38890.1"
                     /translation="MKVPVPIPRIKIGASQRLRCHPCKEKDMVKIDLPRERVKDWKEA
                     MLKKMAEVLEKYRSVRLFMDICVKCGACADKCQFFLGTGDPKNMPVARADLFRKVYRR
                     YFTLGGRVWGRANEAEDLTDSILQDWYTYFYQCSECRRCSVFCPYGIDTAEITMAARE
                     VMAAAGIATKYVTEVVAKVYDTGNNLGIWPPAWKDNCAFLEEDLKDTEGLDIRFPVDE
                     VGAEVLLIPPSADNFVNTDTMIGYAKMFHGAGISWTTSTYCNEGGNFGLFLNYANLKK
                     VNNRILEAARDLKVKKIYWGE"
     gene            complement(1152..1946)
                     /locus_tag="VEL68_22875"
     CDS             complement(1152..1946)
                     /locus_tag="VEL68_22875"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HXZ38891.1"
                     /translation="MAEKFFFIFLPYLSAAIFLIGVLYRFWVWGRTPVPLRIVTTPAP
                     RTRGGVIWRLIGDALWFPSLFQGDRFLWAAGLAFHILLWLVLLRHLRYFLYPVPGWVE
                     GIQDLGLYAGYLIPVSLALLLARRLADEKVLYVSILGDYFSLFLVLGITLSGTLLRVF
                     FRTYLVEVKAYGLSLIHLQPVIPQVHWLFALHFFLVLVLLIYFPFSKLMHSGGLFWSP
                     ARNQRGNFETRFVNPWDFPVAYNPQNLWPPEKYHQALAESKEEEKE"
     gene            complement(2083..2676)
                     /locus_tag="VEL68_22880"
     CDS             complement(2083..2676)
                     /locus_tag="VEL68_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HXZ38892.1"
                     /translation="MATYTLNTPLTEEAVRKLKVRDTVLLNGTIFGIRDLTLIHIFDK
                     RNDPPVSLKGAVLLHTAPSLKMAEDHWEPICVGTTTSGRMDRFTPGLMEKYGVRAIIG
                     KGGQYQASLEAMKKHGGVYLAIVGGAAAVETKQIVKVEKIYFEELHPEALYQFKVKDF
                     GPLTVAMDSHGNHLYFEVKAQAEKKLPGIYKRLGIPV"
     gene            complement(2777..>3013)
                     /locus_tag="VEL68_22885"
     CDS             complement(2777..>3013)
                     /locus_tag="VEL68_22885"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXZ38893.1"
                     /translation="SHNRDPFFAKMEDELYEAINSLGVGPMGLGGDVTTLAVHIEWAH
                     THITQNPVAVNTQCWPARRARAKIYPDGRVEYGF"
BASE COUNT          660 a          854 c          839 g          660 t
ORIGIN      
        1 gtgcatttcg gtaaagtgat tgcagaccgc gcgaagaata tttcgcggcg gctcgaaata
       61 ccccgcggcc ctggccacgt tgcagggatc gtgataggtg acccggaagt catcgttggc
      121 cgacttgtcg atcttgaaag ctccgctgga gagcagtttc caggttagat cgcagatgtg
      181 cgccgggtag gggatttcca gaaaatccat ggctccgctt agagtcgggg tgaaggccag
      241 accggccctc cacgcgtgcc ctcactcccc ccagtagatt ttcttaacct tcagatcgcg
      301 ggccgcttcc agaatccggt tgttcacctt cttcaggttg gcatagttca aaaagagccc
      361 gaaatttcct ccctcattgc aataggtact ggtggtccag gagatgccgg ccccgtggaa
      421 catcttggca tacccgatca tagtgtcggt gttgacgaag ttgtccgccg agggcggtat
      481 cagcaagacc tcagcaccca cctcatcaac cgggaatctg atatccagcc cttccgtatc
      541 cttcaggtct tcctccagga aggcgcagtt gtccttccac gccgggggcc agatccccag
      601 gttgttaccc gtgtcgtaga ccttggccac aacttcggtg acgtatttgg tggcgattcc
      661 cgccgcggcc atcacctccc gggcggccat cgtgatctcg gcggtatcga ttccgtaggg
      721 gcagaatacc gagcagcggc ggcattcgct gcactgatag aaataggtgt accagtcctg
      781 gaggattgag tcggtcaggt cttcggcctc gttggccctg ccccagaccc ttccccccag
      841 ggtgaagtac cgtcggtaaa cttttcggaa gagatcggcc cgggccacgg gcatgttttt
      901 ggggtcccct gtgcccagga agaactggca cttgtcggcg caagccccgc attttacaca
      961 aatgtccatg aagagccgca ccgatcgata tttctccaga acctcggcca tcttctttag
     1021 catggcctct ttccagtcct tgacccgctc ccggggcagg tcgattttta ccatgtcctt
     1081 ttctttgcag ggatgacagc gcaggcgctg agacgccccg atctttatcc ggggaattgg
     1141 aaccgggact ttcattcctt ttcctcttcc ttcgactccg ccagggcctg atggtatttt
     1201 tccggcggcc agagattctg gggattatag gccaccggaa aatcccaggg gttgacaaac
     1261 cttgtttcga aattgccccg ctggttcctg gcaggactcc agaaaaggcc gccggaatgc
     1321 atcagcttgc tgaaaggaaa gtagatcagg agaaccagga ccaggaagaa atgaagtgca
     1381 aagagccagt gaacctgggg gatcacgggt tgaagatgaa tcaagctcag accataagcc
     1441 ttcacctcca ccaggtaggt ccggaaaaag acccggagaa gggttccgct cagagtgatc
     1501 cccaggacga gaaacagaga aaaataatct cccaggatcg aaacgtacag caccttttcg
     1561 tccgccagcc ggcgggccag aaggagcgcg agggagacgg gaatcaggta cccggcgtag
     1621 agccccaggt cttgaatccc ctcaacccag ccgggaaccg ggtagagaaa ataacgcaga
     1681 tgccggagaa ggacgagcca tagcagaata tggaaggcca atcccgcggc ccagaggaag
     1741 cggtctcctt ggaagaggct gggaaaccag agggcatctc cgatcagtcg ccatatcact
     1801 ccgccccggg tccggggtgc cggagtggta acgatccgca gaggcaccgg ggttcttccc
     1861 cagacccaga agcgatagag aacgccaatc aggaagattg ccgcgctcag atagggaaga
     1921 aagataaaaa agaatttttc tgccatttat aaatctaacc gcctccccgc tgtcccccat
     1981 ccagaaggct cgatttaatc atctatttta atatgttttc cttctcccct tgcaggaaag
     2041 ggcaggataa gggggaggga cagggcgggg tgtgttgtga ttctaaacgg gaatccccag
     2101 acgtttgtaa atccccggga gcttcttctc cgcctgggcc ttcacctcga agtagaggtg
     2161 gttcccgtgg gagtccatgg ccacggtgag aggcccgaaa tctttgacct tgaactggta
     2221 gagggcttcc ggatggagtt cctcgaagta aatcttttct accttcacga tctgcttagt
     2281 ctccacggcg gcggccccgc ccacgatggc aaggtagact ccgccatgtt ttttcatcgc
     2341 ttccaggcta gcctggtact ggccgccctt gccgatgatg gcccgcaccc cgtatttctc
     2401 catcagcccg ggggtaaaac ggtccatccg cccgctcgtc gtggtgccga cgcaaatcgg
     2461 ctcccagtgg tcttccgcca ttttcaagct aggagcagta tggagaagga ccgccccttt
     2521 caaggaaaca ggaggatcat tccttttgtc aaaaatatga atcagggtga ggtcccggat
     2581 cccaaagatg gttccgttca agaggaccgt gtcgcgaacc ttcaatttcc tcaccgcttc
     2641 ttccgtcagg ggggtgttta acgtataggt agccatctcg gtctcctttc aaccagaatc
     2701 cagaagtcag gagtcagatt cagaatcgtc atttcctatt ctggctcctg gatgctgact
     2761 tctgaattct acagcttcag aatccgtact ccaccctccc gtcagggtag atcttagccc
     2821 gggcgcggcg ggccggccag cattgcgtgt tcacggccac cgggttctgg gtgatatggg
     2881 tgtgggccca ttcaatatgc accgccaggg tggtcacgtc gccgcccagt cccatgggac
     2941 caactcccag ggaattgatg gcctcgtaga gttcgtcttc cattttggca aaaaagggat
     3001 cacggttgtg gga
//
LOCUS       DASPRR010000625        15036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_154522, whole genome shotgun sequence.
ACCESSION   DASPRR010000625 DASPRR010000000
VERSION     DASPRR010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15036
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154522"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(513..3350)
                     /gene="uvrA"
                     /locus_tag="VEL68_22890"
     CDS             complement(513..3350)
                     /gene="uvrA"
                     /locus_tag="VEL68_22890"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXZ38894.1"
                     /translation="MSDKIIIRGARQHNLKNISLEIPRDRLVVITGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLEQMEKPDVDTIEGLSPAISIEQKTTSKNPRSTVGTI
                     TEIHDYLRLLFARAGRAHCYQCGKEIQSQTIQQIVDQVMSLPPGTRLQLFSPITRGRK
                     GEYRKEFDYLRKSGYARVRVDGTVRDLNEEIALDKNKKHTIDVLVDRLLVKEGAGKRL
                     ADSLATALNLSEGIVKVEVGGGEELVFSEKFACVDCGISYPEISPRMFSFNNPYGACP
                     QCDGLGTTNYFDPDLIVPDKEISIREGAMVPWQGRNSMYFHQTLQSLADHYHIDLYAP
                     FKKLPANVQKVLLYGSNGEQIKFHYEREDRRHFYYRDFEGIIPNLERRYRETDSEIIR
                     EEMEKYMNIRPCPACQGARLRKESLFVKVGGLNIHEVTEKSIRQALDFFTHLELNPRE
                     REIARRILKEVSERLGFLLNVGLDYLTLGRSSATLSGGESERIRLATQIGSGLVGVLY
                     ILDEPSIGLHQRDNARLLSTLKKLRDLGNTILVVEHDQETILEADYVLDLGPGAGAHG
                     GELVFKGTPQELLQSETSLTGKYLSGKLKIPLPHRRRKSRGQFLTLIGASENNLKDIT
                     AKIPLGLFTCVTGVSGSGKSTLILDTLYRALAQKIYHSKERPGKVREIQGLSEIDKVI
                     EIDQSPIGRTPRSNPATYTGLFTPIRDLFAQLPESRARGYAAGRYSFNVKGGRCEACG
                     GDGIIKIEMHFLPDIYVTCEVCRGKRYNRETLEVQYKGKNIWDVLDMTVEQALKFFEN
                     IPSIRAKLETLWDVGMGYIKLGQQATTLSGGEAQRVKLSRELSKRSTGKTVYILDEPT
                     TGLHFDDIRKLLNVLNRLVDSGNTVIVIEHNLEVVKTADHIIDLGPEGGDGGGQVIAV
                     GTPEELAQVPHSYTGQFLKKVLERDRALSAA"
     gene            complement(3607..4635)
                     /gene="pdxA"
                     /locus_tag="VEL68_22895"
     CDS             complement(3607..4635)
                     /gene="pdxA"
                     /locus_tag="VEL68_22895"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXZ38895.1"
                     /translation="MKLETRNWKPIVAITMGDPRGVGPEVVVKALSREGLHQVCRPLI
                     LGDPLILRRTVKALGLRLEVVELEEGSIVERPEGGLGILPLSRLLSGKEPTEVAPEES
                     SRASFRYLEKAGRMALAGQVEAITTGPVSKGAIAQAGIPFQGHTEFFAGLAGTKDFVM
                     MLVGERLRVALVTTHLPLNEVSKSLKEEKILSVVEITGRSLKEFFGIPNPRIAVAAFN
                     PHGGEGGLFGDEEEIISRAVDQARGRDLAVSGPWPADSLFHRAAQGEYDAVVCMYHDQ
                     GLIPLKLLHFDTAVNVTLGLSFIRTSVDHGVAYDIAGKGIASSRSMEEAIRMAAQMAL
                     KKREKSKR"
     gene            complement(4746..5714)
                     /locus_tag="VEL68_22900"
     CDS             complement(4746..5714)
                     /locus_tag="VEL68_22900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXZ38896.1"
                     /translation="MRFKIVPFLVFSLWVLAPRGGPGEVVERIVALVNNQVITLSELD
                     EMGKPVLDQVRQTGLPAEREERLKKARREVLGHLIESKLLEEEMKKRKIEVPDRDVDA
                     AIQDVLKSSNLTEDALKKTLAREGMTYASYRQKIRDELGKMRLVSREIKSKIVIEDED
                     VRKRYQENPEKYSEPLEVQIQQVFFPLPKNATNEQVAAVQREASTILERARRGDDFTQ
                     LAKVYSRGPEASEGGVLGYFKKHELVREVEEAGFKLKPGEISDLVRSSAGFHILKVLD
                     RKGGELRPFAEVQNRIREEAMQEESEKKFNEWMTDLKSKAYIEIRL"
     gene            complement(5748..9227)
                     /gene="mfd"
                     /locus_tag="VEL68_22905"
     CDS             complement(5748..9227)
                     /gene="mfd"
                     /locus_tag="VEL68_22905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HXZ38897.1"
                     /translation="MNPHHLFGPLLHFLEKREGLLRVHGLKGSSPAFLLSRIFGQAPG
                     PLVVISADEEKADRLFQELLFFIGQDESVFLYPSWEVKPFERISPQAEAMGQRWTVRS
                     RLLSNPKKLILVASVRAVLQRVPPRALHSTQAFSLSPDQEFRREELIRRLEGMGYSRS
                     SLVTEVGEFAVRGFLVDLFSPSGPGPVRIEYSGDTVDSVRLFDPESQRSSEALEEAQV
                     LPVKEVLLYPESFEGAARKLSARQQEDPSLQDSARHLLERIREGIPFPGVEFFLPYFY
                     PALDTFFDFFSPRTTLFLEDPSEIGEELIRFWEEAEDAWQNALSREELWPSPGELFLK
                     EEEFQEWGRYYPRVAFELLEAGEEGERLRLDTLSNERLRSELLSSKSDEGILRLLAQR
                     IRSGREKGVVTLLACFSLRSAQRLAEMLEKHDLSVQILDKRFSDWRPEDSEEWEVTLL
                     IGNLSRGFVFPPASLALITETELFGEKQLRKRPAVSVKDHALAAFSDLQVDDYVVHTE
                     HGIGIYRGLWKLHLGKEEHDFLLLEYQDGDKLYLPVYRLNLLQKYVGGGDGGPRLDKL
                     GGTSWEKAKKRVKKSLRELAEELIQLYAARSVVKGHGFSPPDQFLKEFEASFEYEETP
                     DQMQAIEDMQSDLTQEKPMDRLVCGDVGFGKTEVALRAAFRVMMEGKQVAVLVPTTIL
                     AQQHHQTFSRRFAPYPFRVEMLSRFRSRAEQKQVLADLSKGKVDLIIGTHRLLQKDVV
                     FKDLGLLVIDEEHRFGVGHKEKIKRMRTNVDVLTLTATPIPRTLQMSLGGIRDLSVIE
                     TPPAERQAIRTYVTEFDEEVIRDAIRRELRRGGQVFFVHNRVRSIGAMEMFLRRLVPE
                     ARLAVAHGQMEERDLERVMLAFVKKEVDLLLTTTIIESGLDFPSANTILINRADKLGL
                     AQMYQIRGRVGRSKEKAYAYLMVPALSQMGTDARKRLEALQEVSELGSGLKLAMHDLE
                     IRGAGTLLGDAQSGHIAAVGFDMYLQILEQAVRELKGEEVKEEVDPEIDLPIPAFFPP
                     DYIEEINQRLVFYRRLASAKSEVEVQDIREELRDRFGSIPPAAENLFQVMNLKLLLKQ
                     TGIRRISAEKEKVVLTFDSKSSVDPQRLVTAVARGKGRREFTPEQRLKLRPTAKGWEG
                     MLEETKKLLMELL"
     gene            complement(9298..10038)
                     /locus_tag="VEL68_22910"
     CDS             complement(9298..10038)
                     /locus_tag="VEL68_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXZ38898.1"
                     /translation="MSGSQDLSLYIAFGAGLLSFLSPCVLPLVPSYLAFITGLSFEEL
                     TQEENKGRRRRTIFLHSLMFGLGFSTLFTALGASATYLGQFLSAHRDTIRVAGGILII
                     LFGLFISGVFSFNFLQREKKLHLQSKPLGFLGSFLVGLTFAAGWTPCVGPILSSILLF
                     ASTQQEIWSGILLLLAYSLGLGVPFLICSLALNTFLNTFRKTARHMGVITKVGGALLI
                     LVGIILLTDSFSYLSELFNQLFPGASVG"
     gene            complement(10035..11114)
                     /locus_tag="VEL68_22915"
     CDS             complement(10035..11114)
                     /locus_tag="VEL68_22915"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXZ38899.1"
                     /translation="MQMYFRVRHFDRYVGILVVLAIILAIVALVFVGRGQKWFAKRVL
                     YKAVFSKVQGLKPGTPVTISGMEIGNVKSLRLNPQSRVELMIEILQHYQGNIREDSQA
                     TIAMALIGGKTVEITVGSPEKPSLPPGSTIPTLEPKEITDILKEIDLKGSLKKIDEVL
                     DNLKSITHKLDDPKGSLFSTLGNLEFVTSQLKNGQGGVGAILQDPKMHGEINAAIAQV
                     RQSLANVEEITRNAAQVSQNLPPLMGEVDRSVKEIPNLIEDLKKALAELPPILEDVKK
                     TTAHTPAIAENVKEITGNVRKASPQLPDLMTTTQETTEDTDKLIQGLQNHWLLRGSMP
                     KGPREMSPAVGQRESPYEKTGETNR"
     gene            complement(11347..12078)
                     /locus_tag="VEL68_22920"
     CDS             complement(11347..12078)
                     /locus_tag="VEL68_22920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ38900.1"
                     /translation="MEPAIQVRSVSLRIDRRVIFDDLEFSLAKGEALFIVGGSGSGKS
                     LLLRICAGLVVPETGRVTLGGVDLQAATKKEVQDLRARIGFVFQDSALISNMAVYDNI
                     ALPLRYHKKWPEKEVQARVEEKMGLFGVDRTIDWSIPAMLSLEMRKRAALARAFILDP
                     EYLLLDQPTSGLETDTAESLNLIIRDYQQRTGTSLLEVGSEYSLSGGHADRIGVLEDG
                     RITAEGTVEEMQSFLEKGNGPGNSQ"
     gene            complement(12172..13011)
                     /locus_tag="VEL68_22925"
     CDS             complement(12172..13011)
                     /locus_tag="VEL68_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXZ38901.1"
                     /translation="MKIRCWGARGSIPVSGGDYVAYGGDTTCIELRTQEDEIIIVDAG
                     SGIRRLGNLLIREKRDRISMIFTHAHWDHILGFPFFKPAYRKGTAIQMFGCPLAQESI
                     QGMISRTMEAPYFPVNFQDLKADISYHEACESSFSIGSVTVTPIPLSHPNQGYGYKFT
                     ENGKSFVFLTDNELAFQHPGGRTFPEYVGFCRQADFLIHDAEFTEKEYRWTRSWGHTV
                     YTDALRLALEAKVEKFGLFHHNQERTDAALEGIVENCRRIIAKREERMECFALSQQTE
                     IQL"
     gene            complement(13074..13874)
                     /locus_tag="VEL68_22930"
     CDS             complement(13074..13874)
                     /locus_tag="VEL68_22930"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ38902.1"
                     /translation="MNEKRNSLDSIGGWVRGKGTVLLNLCALLYTAFRELVAERKKGF
                     SLVLEITLRQVYFTGVQALRVVAVISLALGTVIIVQIGTQLSFLGGGIEFIVPILVLI
                     LFRELGPLLTAIIVIGRSGTAIATELGNIVIAHELEAIEVMGINPVYFIVTPRIIGVT
                     VAVISLTIIFIAVGLLGGFWVSKLILPITFDAFLRELEVALSAADLLAGFLKSLIFGV
                     LIALTCTYYGLTVRYSSIEVPQAATRGVVSAMLFCFATNALLTVLFYL"
     gene            14174..14632
                     /locus_tag="VEL68_22935"
     CDS             14174..14632
                     /locus_tag="VEL68_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXZ38903.1"
                     /translation="MDKIVPVEKSIFTLGTSNRTWEEFINLLRAHKIEMVIDVRSFPT
                     SHFAHFKRESLNPSLAEAGFGYYYLGKELGGYRKGGYEAYTQTLPYLVGIELLERMSS
                     RCRSAILCAERFPWRCHRRFIGRTLQERSWGVTHIIDDKRVWKDQVLEEP"
     gene            14714..>15036
                     /locus_tag="VEL68_22940"
     CDS             14714..>15036
                     /locus_tag="VEL68_22940"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXZ38904.1"
                     /translation="MALVLFEKKEETLRVSLNRASALNAINSPILKELEQGLKEHEFD
                     SRLKGLVLFGQGGCFSSGADIRELAGLDEEGVRKFHELRERTFKLLEKYPYPTLAVIE
                     KYALGT"
BASE COUNT         3193 a         4248 c         4252 g         3343 t
ORIGIN      
        1 ccgcttcagc cagacgcggt tccgcaggcg ctgggaaagg acgggtgcat agtcgaggga
       61 ggtttcccgg gctacgtcat aaacccgagc gttcagaatt ttcttcaagt atttctgcgg
      121 caacttcgtc ctcctctttc cttgatcctc ttccccggca tcaaaaccgg gcggtcatgc
      181 ccgcctttgg cggattgaca tttccagaat gcctggctcg tggcccgggg gcaagggcat
      241 tacgcgcaga aagcatcggc ctgcccattt cggatttcga attgttgatt tcggattttg
      301 gggtttcttt tccaatccgc attccgcaat caacaattcg caatttggga ggcccgcccg
      361 gtccatgaaa tggaagctgc ctcatgattt ttgcccagaa ggtccttgtc ccagagccgg
      421 gcaacagaga cccattcaga attagctttg agcggatagc tgcctttcaa gcctttgtct
      481 ctgccggagg tcatgactat cgcccggggg aatcaagccg cgctgagggc tcgatcacgt
      541 tctaggacct tcttcaggaa ctggccggtg taggagtggg ggacctgggc taattcttcc
      601 ggggttccca cggcgatcac ttgtccgccg ccgtctcccc cctcggggcc aaggtcgatg
      661 atgtgatcgg cggtcttgac gacctccagg ttgtgctcga tgacgatcac ggtattgccg
      721 gaatccacca gccggttgag aacgttcagg agcttgcgga tgtcgtcgaa gtgcaggccg
      781 gtggtcggtt catcgaggat gtacaccgtc ttgcccgtgg agcgtttgga aagctctcgg
      841 gagagcttga ccctctgggc ttcaccgccc gaaagggtgg tggcctgctg ccccagcttg
      901 atgtagccca tgcccacatc ccaaagggtt tccagcttgg cccggatgga ggggatgttt
      961 tcgaaaaact tcagggcctg ctccacggtc atgtccagca cgtcccagat atttttccct
     1021 ttgtactgga cttccaaggt ctcccggttg taccgcttgc cccggcagac ctcgcaggtc
     1081 acatagatgt ccggcaggaa gtgcatctcg atcttgatga tcccgtcgcc cccgcaggcc
     1141 tcgcaacgtc cccctttgac attgaagctg taccggccgg cagcataccc gcgggctctg
     1201 gattcgggga gctgggcaaa gaggtctcgg atgggggtga agagccccgt gtaagtggcc
     1261 gggttcgatc ggggggtccg cccgatgggg gactggtcga tctcgatgac tttgtcgatt
     1321 tcggagagac cttgaatttc ccggaccttt ccgggtcttt ccttggaatg ataaattttt
     1381 tgggccaacg cccggtagag ggtatccagg atgagggtgc tcttccccga gcccgacacc
     1441 ccggtaacac aggtgaacaa tcccaggggg attttcgcgg tgatgtcctt gaggttgttc
     1501 tcgctggccc cgatcagggt caagaattgg cccctcgact ttcttcgtcg gtggggaagg
     1561 ggaattttca gctttcccga aaggtatttt ccggtgagag aagtttcgga ctggagcagc
     1621 tcctggggag ttcccttgaa gaccaattct cccccgtgag ctcccgcccc ggggccgagg
     1681 tccagaacgt aatcggcctc caggatggtc tcttggtcat gctccaccac cagaatcgtg
     1741 tttcccaaat ctcgcagctt tttcagagta gaaagaaggc gggcattgtc ccgctggtgt
     1801 aagccgatgc tgggctcgtc gagaatgtag agcaccccca ccaggccgga cccgatctgg
     1861 gtggccaggc ggatgcgctc ggattccccg cccgagaggg tagctgaaga acggccgagg
     1921 gtgaggtaat cgagcccgac attcagcagg aaccccaggc gctcagagac ctcttttaag
     1981 attcgccggg cgatctcccg ctctctcggg ttcagttcca ggtgggtaaa aaaatccagg
     2041 gcttggcgga tggatttttc cgtgacttcg tggatgttca acccgcccac cttgacgaag
     2101 aggctctctt tgcgcagccg ggctccctgg caggccgggc aggggcggat gttcatgtac
     2161 ttttccatct cttcccggat gatctccgaa tccgtctccc ggtaccggcg ctccaggttg
     2221 ggaatgatcc cttcgaaatc ccggtaatag aaatgcctcc ggtcctcccg ttcgtaatgg
     2281 aacttgatct gctccccatt ggacccgtag aggaggacct tttgcacgtt ggcggggagc
     2341 tttttgaagg gagcgtagag atcgatgtga taatgatcgg caagggactg gagggtctgg
     2401 tggaaataca tggaattgcg cccctgccag ggaaccatcg ccccttcccg aatggatatt
     2461 tccttgtcgg gaacgatcag gtccgggtcg aaatagttgg tcgtccccag cccgtcgcac
     2521 tgagggcagg cgccgtaggg attgttgaag gagaacatgc gggggctgat ctccgggtag
     2581 ctgattccgc agtccacgca ggcgaatttt tcgctgaaga ccagctcttc ccccccgccg
     2641 acctccacct tgacgatccc ttcggaaaga ttcagggcgg tagccagcga gtcggcgagc
     2701 cgctttccgg ccccttcctt caccaggaga cggtccacca ggacatcgat ggtgtgtttc
     2761 ttgtttttgt ccagggcgat ctcttcgttc aggtcgcgga ccgtcccgtc caccctcacc
     2821 cgggcatagc cgctcttgcg caggtaatcg aactcctttc ggtactctcc tttgcggcct
     2881 cgggtgatgg gggaaaagag ctgcagacga gtgcccgggg ggagggacat gacttgatcc
     2941 acaatctgct ggatggtctg agactggatc tccttgccgc actggtagca gtgggcccgc
     3001 cccgcgcggg cgaagagcag gcgaaggtag tcgtggattt cggttatggt gccaaccgtg
     3061 gagcgagggt tcttggaagt ggtcttctgc tcgatggaga tagccggaga aaggccctca
     3121 atggtatcca cgtcgggctt ttccatctgt tccaggaact ggcgggcgta ggccgaaagg
     3181 gactccacgt aacgccgctg cccttcggcg tagagagtat cgaaggccag ggatgatttc
     3241 cccgacccgg aaagaccggt gatcaccacc agccgatccc gggggatttc caggctgatg
     3301 ttctttaagt tgtgctggcg ggctccgcgg atgatgattt tatcggacat aaccttctaa
     3361 aatccgaaat tcgaatgtcg aaatccgaaa caaattcgga atcaaaacgc ccgaagactt
     3421 gacgaactcg taaaaattcg aaattccatg aaatttgtca tcccagcgaa ccccggatcg
     3481 ggtccggggc aggcgccaga atccagcaat ttcaagatgt cctggacccc gtctttcgcc
     3541 ggggtgacgg ctttcgaatt ttggtcattc gtgcttgttt cgaaattcgt gtttcgatat
     3601 tcggatttac ctcttcgatt tttctctctt ctttagggcc atttgggctg ccattctgat
     3661 ggcctcttcc atggaccggg aactggcgat ccctttgccg gcaatatcat aggccacccc
     3721 atggtccacc gaggtgcgga taaaggaaag gccaagggtg acattcacgg cggtatcgaa
     3781 atggagaagc ttcaggggga tcaggccctg gtcgtggtac atacagacca ctgcgtcgta
     3841 ctctccctgc gctgctcgat ggaagagtga atccgccggc cacggacccg agacggccaa
     3901 atctcttccg cgggcctgat cgaccgcccg agagatgatc tcttcttcgt cgccgaagag
     3961 cccgccctcc cccccatgtg ggttgaaggc ggcgaccgcg attcggggat tcgggattcc
     4021 aaaaaattct ttcaggctcc gcccggtgat ctcgacaacc gaaaggattt tttcttcctt
     4081 caaagatttg ctcacttcat tcagcggtag atgggtggtc accagagcta ctctcaaccg
     4141 ctccccgaca agcatcatga caaaatcctt tgttcccgca aggccggcaa agaattcggt
     4201 atggccctga aagggaattc cagcttgggc aatcgccccc ttgctcaccg gacccgtggt
     4261 tatggcttcg acttgtccgg ccagagccat tctcccggcc ttctccagat agcggaaaga
     4321 ggcccgggaa ctctcttccg gggcgacctc cgtcggttct tttcccgaaa ggagacggga
     4381 aagaggaaga atccctaacc ctccctcggg cctttcgaca atcgatccct cctcgagctc
     4441 gacgacctcc aaccttagtc ctagggcctt cacggttctc cggaggatga gcgggtctcc
     4501 cagaatcaag gggcggcaaa cttggtgaag gccctcccgg gacagggcct tcaccaccac
     4561 ttccgggcca accccccggg ggtctcccat agtgatggca acaatgggtt tccagtttcg
     4621 agtttcaagt ttcaagtttt aggcttcaga ttgcgggcgg ctcatgacga aattctgggt
     4681 ccaagtttcg ggttgcagaa aaagatctta acctggaacg ggaaaccggc accgcgggaa
     4741 aacggtcata gtcttatttc aatgtaggct ttcgacttca agtccgtcat ccactcattg
     4801 aatttcttct cggattcttc ctgcatggcc tcttcccgga ttcggttctg aacttccgcg
     4861 aaaggacgga gttccccgcc cttccggtcc agcactttta agatgtgaaa tcccgccgag
     4921 ctcctgacca gatcgctgat ttcccccggt ttcagcttga atccggcttc ctccacctct
     4981 cgaactaatt cgtgtttctt gaaatagcct agaacccctc cctcgctggc ttcggggcct
     5041 cgcgaataga ctttggccaa ctgggtgaag tcatcccccc tccgggctct ttccaggatg
     5101 gtcgaggcct ccctttggac ggcggcgact tgctcattcg ttgcattttt agggagaggg
     5161 aaaaagacct gctggatctg gacctccaga ggttcagaat atttttccgg attttcctga
     5221 tatcgcttcc tcacatcttc gtcctcgatg acgatcttcg atttgatttc ccggctgacg
     5281 aggcgcatct ttcccagctc atcccggatt ttctgccggt aggaagcata ggtcatccct
     5341 tcgcgggcaa gggtcttttt gagcgcatcc tcggttaggt tcgaggactt caggacatcc
     5401 tggatggccg cgtccacatc ccggtccggg acttcgatct ttctcttctt catttcttct
     5461 tccagaagct tgctctcgat caagtgaccc aagacctccc tccgggcttt tttcaatcgc
     5521 tcttccctct ccgcaggaag accggtctgc cgcacctgat cgaggacggg cttgcccatt
     5581 tcatccaact cggaaagagt gatcacttgg ttgttgacca gggctacaat ccgctcgacc
     5641 acctctccag gaccgccacg gggagccagg acccacaggc taaataccaa gaaaggcaca
     5701 attttgaatc tcataatcct agggagcata aaaaataaat tatcatttca gaggagctcc
     5761 atcaataatt ttttggtttc ctccagcatc ccttcccatc ctttggccgt cggccggagt
     5821 ttcaagcgct gttctggagt gaattctctc cgtcccttgc cccgggccac cgcggtcacg
     5881 aggcgctgag gatcgacgga agatttcgag tcgaaggtga gcactacttt ttccttttcc
     5941 gccgaaatcc gtcgaattcc ggtctgcttc aaaaggagtt tcaggttcat gacttggaag
     6001 aggttctcgg ccgcgggagg gatggagccg aaccggtccc gcagctcttc acggatgtcc
     6061 tggacctcca cttcggattt cgcggaagcc aagcggcggt agaagaccag gcgctggttg
     6121 atttcttcaa tgtagtccgg ggggaagaag gcggggatgg gcaagtcaat ctccgggtcg
     6181 acctcttcct tcacctcttc ccccttgagc tcccggacgg cttgctccag gatctgcagg
     6241 tacatgtcaa accccacggc agcgatgtgc ccggactggg catctcccag gagggtcccc
     6301 gccccccgga tctccaggtc gtgcatggcc agtttcaagc cggaccccag ctcgctcacc
     6361 tcctggaggg cttccagcct cttgcgggca tcggtcccca tctgggagag ggccgggacc
     6421 atcaggtagg cataagcctt ttctttggat cggcccaccc ggccgcggat ttggtacatc
     6481 tgggccagcc ccagcttgtc cgcccggttg atgagaatgg tgttggccga ggggaaatcc
     6541 aggccggact cgatgatggt ggtggtcagg agcaaatcca cttccttctt gacaaaggcc
     6601 agcatgaccc gctccaggtc cctttcttcc atctggccgt gggccacggc gaggcgcgcc
     6661 tccggaacca gccggcgcag gaacatttcc atggccccga tggatctcac ccgattgtgg
     6721 acgaagaaaa cctgtccgcc ccgccgcagt tcccgccgga tggcgtcgcg gatgacctcc
     6781 tcatcaaact cggttacgta ggtgcggatc gcctgccgtt ccgcaggggg agtttcgatc
     6841 acgctcaagt cgcggatccc gcccagagac atctgcaggg tgcgggggat gggggtagcg
     6901 gtcagagtca ggacatccac attcgtacgc atgcgcttga tcttttcctt atgccccact
     6961 ccgaagcgat gctcctcatc gatcaccagg agacccaggt ctttgaaaac cacatccttc
     7021 tgcagcaaac ggtgggttcc gatgatcaaa tcgacctttc ctttggacag atcggccaga
     7081 acctgcttct gctcggcccg ggaccggaac cggctgagca tctccacccg gaaggggtaa
     7141 ggggcgaacc ggcgggaaaa agtctggtgg tgctgctggg ccaggattgt ggtgggaacc
     7201 agcacggcca cctgctttcc ctccatcatc acccggaaag cggcccgcag ggccacttcg
     7261 gtctttccaa atcccacatc cccgcagacc aggcggtcca tgggtttttc ctgggtcagg
     7321 tcgctctgca tgtcttcaat ggcctgcatt tggtccgggg tttcctcata ctcgaaggag
     7381 gcctcgaact ccttcaagaa ttgatccggg ggggagaacc cgtgcccttt caccaccgaa
     7441 cgggcggcat agagctggat gagctcctcg gccagttccc gcagggattt cttcacccgt
     7501 ttctttgctt tctcccaaga ggtcccgccc aacttgtcca gacggggccc gccgtcgccc
     7561 cctcccacgt atttctgcag gaggttcaag cggtatacgg ggagataaag cttgtccccg
     7621 tcctggtatt caaggagcag aaagtcatgc tcctcctttc caagatggag cttccacagc
     7681 ccccggtaga tgccgatgcc gtgctcggta tggaccacgt aatcgtccac ctggaggtcg
     7741 ctgaaagccg ccagggcgtg gtctttcacg gagacggcgg gacgcttccg gagctgtttt
     7801 tccccgaaga gctcggtctc ggtgatcagg gccaggctgg cggggggaaa gacaaaaccc
     7861 cgggatagat ttccgatgag aagggtgacc tcccattctt cggaatcttc cggcctccag
     7921 tcggaaaagc gtttgtccag aatctgcacg gagaggtcgt gcttctccaa catctccgcc
     7981 aaccgctggg ccgagcgcag gctaaaacag gccaggagag tgaccacacc cttctcccga
     8041 ccggagcgga tgcgctgggc gaggagccgc aggatccctt catcggattt ggaggatagg
     8101 agttccgacc tgaggcgctc gttggaaagg gtatccaggc gtaggcgctc tccttcctcc
     8161 cctgcctcca gcaattcgaa ggcgacccgt ggataatatc ggccccattc ctggaactct
     8221 tcctccttca gaaaaagctc cccgggggac ggccatagtt cctcccggga aagagcgttc
     8281 tgccaggcat cttcggcttc ctcccagaag cgaatgagct cttccccgat ttccgagggg
     8341 tcttccagga agagggtagt ccgcggggaa aagaaatcga agaaggtatc cagagcaggg
     8401 tagaaatagg ggaggaagaa ttccacgccg gggaagggaa tcccttcccg aattctctct
     8461 aggaggtggc gagccgaatc ctggagcgaa ggatcctctt gctggcgggc ggaaagcttc
     8521 cgggcggctc cttcaaatga ttccgggtag agcaggacct ctttcaccgg caggacctgc
     8581 gcctcctcga gggcttccga ggagcgctgg ctctccgggt caaagaggcg caccgagtca
     8641 accgtgtccc cggaatattc aatgcgcacc gggccaggcc cggaggggga aaagaggtcg
     8701 accaggaacc cccgtacagc gaattccccg acttccgtaa ccaggctgct ccggctatag
     8761 cccatccctt ccaagcggcg gatcaattct tcccgcctga actcctgatc cggggaaaga
     8821 gaaaaggcct gagttgaatg aagggcacgc gggggaactc tttgaaggac ggcccgtacc
     8881 gaggccacca ggatgagctt tttcgggttg gaaagcaggc gggacctgac ggtccaccgt
     8941 tgacccatgg cttccgcctg gggggagatt ctctcgaaag gtttcacctc ccaggaggga
     9001 tagaggaaaa cgctttcatc ctgtccgata aaaaagagca gctcctgaaa gagccggtcc
     9061 gccttttctt catcggccga gatgacaacg agggggcctg gggcctggcc gaagatgcgg
     9121 gataggagaa aagcgggtga ggaccctttc aggccgtgga cccggagcaa gccttcccgc
     9181 ttttccaaaa aatgaagcag gggaccaaac agatgatgag gattcatttc cctacccacg
     9241 gaaattttcc cccccagttc ctatccaagg tcatcgggat tcggtccgga agatgggcta
     9301 accgacggag gccccaggga agagctggtt aaacagttca ctcaggtagg agaaagaatc
     9361 ggtcagcaga ataatcccca cgaggatcaa aagagcgccc ccgaccttgg tgatgactcc
     9421 catatgcctg gcggtcttcc gaaaggtgtt caggaaggta ttcagcgcca gggagcagat
     9481 caggaaaggc acccccaggc ccagagagta ggcgaggagg aggaggattc ccgaccagat
     9541 ttcctgttga gtgctggcaa atagaaggat ggaggaaaga attgggccca cgcagggagt
     9601 ccatccggcg gcaaaggtca accccaccag gaaagacccc aggaatccca ggggcttgct
     9661 ctggaggtgt aatttcttct cccgctggag gaagttgaaa gagaagaccc cgctgatgaa
     9721 aagcccgaaa agaatgatca gaattccccc cgcgacccgg atggtatccc ggtgggccga
     9781 caggaattgc ccgagatagg tggccgaggc cccaagagcg gtgaagaggg tgctgaatcc
     9841 aagtccgaac atgagagaat gaagaaagat tgtccggcgt cttctcccct tgttctcttc
     9901 ctgggtgagt tcctcaaaag agaggccggt gataaaagcc agatatgatg ggaccaaggg
     9961 caagacgcag ggagaaagga aggagaggag ccctgcgccg aaggcaatat acagggaaag
    10021 atcctgagac ccgctcatcg gtttgtctct cctgtctttt cataggggct ttcccgttga
    10081 ccgaccgccg gagacatctc tcttggaccc ttgggcatcg atccgcgcag gagccagtgg
    10141 ttctggagtc cctggatgag tttgtcggtg tcttccgtgg tctcctgggt ggtcgtcata
    10201 aggtcgggga gctgggggga ggctttcctt acgttcccgg tgatctcttt cacattctcg
    10261 gcgatagcgg gggtatgggc cgtggtcttc tttacgtctt ccaggatggg gggcaattca
    10321 gccagggctt tttttaggtc ctcgatcagg ttggggattt ccttgaccga acggtccact
    10381 tctcccatca gcggaggcag gttctgggaa acctgggccg cattccgggt aatttcttcc
    10441 acgttggcca gagactggcg gacctgggcg atggccgcgt tgatctcccc gtgcattttg
    10501 ggatcctgga gaatggctcc cacgcccccc tggccgtttt tgagctggga ggtcacgaat
    10561 tccagattgc ccagagtgct gaaaagggac cccttcgggt cgtccagttt gtgggtgatg
    10621 gatttcaaat tgtccaggac ttcgtcaatc ttcttgaggg aacccttgag gtcgatctct
    10681 ttaagaatat cagtgatctc cttgggctcc agggtgggaa tggtactccc gggcggaagt
    10741 gagggttttt caggggagcc gacggtgatt tcaactgttt tacccccaat gagggccatg
    10801 gcaatcgtgg cctgggaatc ctcgcggata tttccctggt agtgctgcag gatttcgatc
    10861 atcagctcca ccctactctg gggattcagc cggagggact ttacatttcc gatttccatc
    10921 cctgaaatgg tgaccggcgt tcccggtttg agcccctgaa ccttgctgaa gacggctttg
    10981 tagagaacac gcttggcaaa ccatttctgg ccgcgaccca caaagaccaa agccacgatc
    11041 gccaggatga tggccaggac taccaaaatc cccacgtacc gatcaaagtg tctgactcga
    11101 aaatacatct gcatagattt gccttttcat catgctttca catgaagggg aagaaaacag
    11161 cctcctgagg atgaaggccg gcaaggggaa aagcacccag ctgccggcgc cgaatcgtcc
    11221 gaaagaaccc ctagggtgcc tgaagtgcgc taaaaggcca agagtgaaaa ttcctcaatc
    11281 cagtccccca aattccaagc ctagaatctt ccctatcggt catgaaaacc tgaaatttgc
    11341 gactggctat tgggaatttc ctggcccatt tcccttttcc aaaaaagact gcatctcctc
    11401 caccgtccct tcggccgtga tccggccgtc ttccagcacc ccgatccggt cggcatgtcc
    11461 ccccgaaagg gagtattcgc tgcctacctc cagaaggctg gtccccgtcc tttgctggta
    11521 gtcgcggata atcaggttta agctttcggc ggtgtccgtc tccagaccgc tggtcggttg
    11581 gtccaggaga aggtattccg ggtccaagat gaaggccctg gctagggcgg cgcgcttgcg
    11641 catttccagg cttagcattg ccgggatgga ccagtcaatg gttcggtcca ccccgaagag
    11701 ccccatcttt tcctccaccc gggcctgcac ttccttttcg ggccacttct tatggtaccg
    11761 gaggggaagg gcgatattgt cgtagacagc catgttgctg atgagagcgg aatcctggaa
    11821 cacaaaaccg attcgagccc gcaaatcctg gacctccttc ttggtggccg cctgcaggtc
    11881 aacgcccccc agagtcaccc tcccggtttc cggaacaacg aggccggcgc aaattcggag
    11941 aagaagggac tttccgcttc cgctgccccc cacgatgaag agggcctccc ctttggccag
    12001 ggagaattcc aggtcatcga agatcactct tcggtcaatt cgaagggata cgcttcgaac
    12061 ttggatagcg ggttccatcg gtctgttcgc ggaggaatcc taattcttat attttccttc
    12121 ggctcaagga gatagggaag aagagatccc tccatccccc ggaggatttt attacagttg
    12181 aatctcggtc tgctgcgaga gggcgaaaca ttccatcctc tcttcccgct tggcgataat
    12241 cctgcggcaa ttctcgacga tcccctccag ggccgcatcg gtcctttcct ggttgtgatg
    12301 gaagagtccg aatttctcca ccttggcctc cagggccaaa cggagggcgt cggtatagac
    12361 cgtatggccc caggagcggg tccagcggta ctctttttcc gtgaattcgg cgtcgtggat
    12421 cagaaaatcg gcctggcggc agaaacccac gtattctgga aaggtgcgtc cgccgggatg
    12481 ctgaaaggcc agttcgttat ccgtcaagaa aacgaagctc tttccgttct ccgtaaattt
    12541 gtaaccgtac ccctggttgg gatggctcag cgggatgggg gtgacggtta cggatccgat
    12601 ggaaaagctg ctttcacagg cctcgtggta ggagatgtct gccttaaggt cttgaaaatt
    12661 cacagggaag tagggagctt ccatggttcg cgagatcatc ccctggatcg attcctgggc
    12721 cagggggcac ccgaacatct ggatggccgt tcccttgcgg taggcaggtt tgaaaaaagg
    12781 aaacccgaga atgtggtccc agtgcgcgtg ggtgaagatc atggaaatcc ggtctctttt
    12841 ttctcgaatc aacaggtttc ccagcctccg aatccctgaa cctgcatcga caatgatgat
    12901 ctcgtcctct tgggtccgga gctcgataca ggtcgtatcc ccaccgtagg ccacatagtc
    12961 ccctccggaa acgggaatgg agcccctggc tccccaacag cgaatcttca tcagttatcc
    13021 cttggtccag ttctccggcg cccctttgcc cagggatgcc ggtttctttt cgcttacagg
    13081 taaaagagca ctgtcagcaa agcattggtc gcaaaacaga acagcatggc gcttactact
    13141 ccccgggttg ccgcctgggg aacttcgatc gacgaatacc gaactgtgag cccgtaatag
    13201 gtgcaggtta aggcaatcag cacgccaaaa atgaggctct tcaggaagcc agcgagcaga
    13261 tcggcggccg agagggcgac ctccaattcc cgtaaaaagg cgtcgaaggt gatgggcaga
    13321 atcagcttgg atacccagaa gcctcccagc agcccgacgg cgataaaaat gatggtcagg
    13381 ctgatcaccg caacggtgac cccgatgatc cggggggtta cgataaaata gaccgggttg
    13441 atgcccatta cctcgattgc ctccagttca tgggcgatga cgatattccc cagttctgtg
    13501 gcaatagccg tgcccgagcg tccgataacg ataatcgcgg tgagcaaagg ccccagttca
    13561 cgaaacagga tcaggaccag gatgggaaca ataaactcaa ttccaccccc cagaaaggag
    13621 agctgggtcc cgatctgaac gatgattacc gttcccaggg ccaaggaaat gaccgccaca
    13681 actctaaggg cttgaacccc ggtaaaatat acctggcgca gggtgatctc aaggaccaac
    13741 gaaaaccctt tttttctctc cgccacaagc tctcggaagg ccgtgtaaag aagagcgcaa
    13801 aggttgagaa gaacggttcc ctttcccctt acccatccac cgatggaatc cagagaattc
    13861 cgcttttcat tcaagactag tccttgtggg gcgctgaccg gggaacgcat ggtgatttca
    13921 gagagacctt gaggctttca ctttcctttc catcaggccg gcaaaaaata tattcttaaa
    13981 aaattaatat atcattaacc ttccgaattt tctattcaaa tattgctccc cgggcaggaa
    14041 cgtggtagat aaaggcatag ctcagagcac atccagtcta gcatcgaaat cataataaag
    14101 ggcgcaaacg ggagatatcg caaaccgaag gacaagcaac cttaaccagc aaagaggaat
    14161 cagggcctct tctttggaca aaatagtccc agttgaaaaa agcatattta ctctgggcac
    14221 cagcaatcgg acctgggaag agtttatcaa cctcctccga gcccataaga tcgaaatggt
    14281 gatcgatgtg cgcagcttcc ctacgagcca tttcgcccac tttaaacggg agtccctcaa
    14341 cccttctctg gccgaggccg ggttcggata ctactatctg ggaaaggaat tggggggcta
    14401 ccgcaagggg ggatatgagg cttataccca gaccctccca tacctggtgg ggattgagct
    14461 cctggagcgc atgtcttccc gttgccggtc agccatcctc tgcgccgagc gattcccctg
    14521 gcgatgccac cgcaggttca tcggtcgaac cttgcaggag aggagctggg gtgttaccca
    14581 tatcatcgat gataagaggg tttggaaaga tcaagttctg gaggagccgt aggggcatcc
    14641 tcccccggcg ggacatcgcg tccctacttg gactgggaat cattgaagat ttcataggga
    14701 aaaggtgata accatggccc tggttttatt tgagaagaaa gaggaaacat tgcgggtttc
    14761 cctaaaccgg gcgtcggccc tcaatgccat caatagccct attcttaagg agctggagca
    14821 ggggctgaaa gaacacgaat tcgattctag gcttaagggt ttggttctct tcgggcaagg
    14881 aggatgcttt tcttcagggg ctgacattcg agaattagcg ggtctggacg aagaaggggt
    14941 ccgaaaattt cacgagctaa gagagaggac cttcaagctt ctggaaaaat acccctatcc
    15001 gaccctcgcg gtcatcgaaa aatacgcctt gggaac
//
LOCUS       DASPRR010000626        12475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1440237, whole genome shotgun sequence.
ACCESSION   DASPRR010000626 DASPRR010000000
VERSION     DASPRR010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12475)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12475
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440237"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1571
                     /locus_tag="VEL68_22945"
     CDS             357..1571
                     /locus_tag="VEL68_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXZ38905.1"
                     /translation="MADQDLSKLTIDKSKTAFRPARGKKVLYLVFLLLALLTAGFLYR
                     MEIFTPPIQVQLANVTQIYPSQGFTMLTASGYVVPQRKSALASKVTGRLVWLGVEEGS
                     RIKKGEVVARLESQDVEATKAQAAANLETARFNLENARAELVDATLNLNRSKELVDKN
                     FIARGDYDTTVARYKKAEAAVKAAEAVIRANEAALEAAAIQLEYTLIRAPFDAVVLTK
                     NADIGDIVTPFSASVESKSAVVTIADMDSLQVQADVSESNLGQVKIGQPCEVQLDALP
                     DRRLRGAVHMIVPTGDRSKAAVMVKIRFIDKDPRTLPEMSAKVSFLSREITKNDQRPR
                     TAVSQKALVARGDGKHVFRVQGNRAVQASVLLGNPIGDMVEVIDGLKAGDQVVLDPPA
                     KLKSGAKIKAGE"
     gene            1811..2506
                     /locus_tag="VEL68_22950"
     CDS             1811..2506
                     /locus_tag="VEL68_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38906.1"
                     /translation="MSPIVEIQNLSKSYRRGDRRVPVLQNINLDIQEGEFLALMGPSG
                     SGKSTLLNLIAGLDQVDDGTVIVGGVNITSLSESELAAWRATHVGFIFQFYNLIPVLT
                     AFENVELPLLLTGLSRRERREHVELALRVVNLEARMDHRPPQLSGGEQQRVAIARAIV
                     TDPTILVADEPTGDLDRVSAEEVLSLMERLNRESTKTIIMVTHDPRAAKKAGITRHLD
                     KGLLNDVHSQAPV"
     gene            2481..3638
                     /locus_tag="VEL68_22955"
     CDS             2481..3638
                     /locus_tag="VEL68_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXZ38907.1"
                     /translation="MFILKLLFKNAFRHKLRTSLTLLSIAIAIMAFGLLRTVVSAWYS
                     GVEASSATRLITRNSISLVFPLPLAYKDRIRQVENVTTVSFGNWFGGIYIDRKNFFPN
                     FAVDPKTYLDLYPELILSPEQRQAFLRDRKGAAAGKKLIERFKWKIGDLITLQGTIFP
                     GNWEFVLRAIYRGRDENVDESQFFFHWDYLNEALRKTAPSRADRVGFYMIGVDHPNNS
                     SNVVQAIDRTFKNSLAETLTETEKAFTLSFISMSEAIIVAIQIVSFVVIGIIVAVVAN
                     TMSMAARERTWEYAVLKTLGFGGWHIAGLILGESLLITLMGCALGMAITFPGAYIFKK
                     SVPMYLPVFQVEETTLYLDLVAFLGVGILSALIPAWRAIQVRIADGLRRIA"
     gene            3639..4808
                     /locus_tag="VEL68_22960"
     CDS             3639..4808
                     /locus_tag="VEL68_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ38908.1"
                     /translation="MGLPLSYSIRNLWTRRLTTILTAFGMALVVFVFTAILMMAEGLK
                     KTLVETGSPDNVVVLRKGSSSEVQSGVERIQASIIETQPEAAVGANGRLLLAKEAVVL
                     ISLPKRENSKAVSNVVVRGIGESSLALRPQMRLVQGRMPRLGLPEVMAGQKIAERFRG
                     GGLGETLRFGLRDWTVVGVFDAGNTGFSSEIWGDADQLMQAFRRRVYSSVVFKLHDPG
                     EFLPFKRRIETDPRLNLEAKQETRYYAEQSEIMSKFLNILGLSLTIIFSFGAMLGAMI
                     TMYSSVASRTSEIGTLRALGFQRGSILRAFLYESLFLGLIGGGAGIFLGSFMQLITIS
                     TMNFQTFSELAFSFTLTWEIAWKALAFSLGMGFLGGFLPSMQASRMNIVDSLRAQ"
     gene            complement(5026..6339)
                     /locus_tag="VEL68_22965"
     CDS             complement(5026..6339)
                     /locus_tag="VEL68_22965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ38909.1"
                     /translation="MRIELVSAAAEESARLTPLALATLAALIPPDMEVHFSDDQLSPI
                     DLCNAFPGTDLVAISVLSKTAHRAYEIADACRKKGIQVVLGGIHPTVLPEEASTHADA
                     VVIGEAEILWPRLLEDFRTNHLQKFYRQDRWVDMNRSPAPRRTIFQPYARSYVPLDVI
                     QTTRGCPFHCDFCTVNSIFGSPFRMRDIEKVIAEVRELKRWGILLADDNVIGNVPYFQ
                     KLFTALEPLKLRWIGEASLAGLDQEQNLRILQRSGCKALFIGFESLSPQLKTTGKTQN
                     HPEQYREIIHKLHDHGIIAYAAFMFGFDFDDPSIFERTVEFAIASKIILAQFAMLTPY
                     PGTRFYSRLKAEGRLLRDQWWLLPNQEILAPHFRPLGMEPEQLREGWKWAWKEFYNFS
                     SIWKRVDFWPALHPYLAYLPFNLRQRHFARRKICAENTRPRSWKE"
     gene            6691..7806
                     /gene="ydiK"
                     /locus_tag="VEL68_22970"
     CDS             6691..7806
                     /gene="ydiK"
                     /locus_tag="VEL68_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HXZ38910.1"
                     /translation="MEDKSGKLPEANSPPGRDLTRTTLGVLLIGILIVGNFWILRPFL
                     PSLLWAVMIVVATWPLMLGLQARLWCKRGLAVAVMTLALLLVFVVPFSLAVATIVENA
                     DQIADWAKSLKELALAAPPDWVGRIPLAGPKISAAWQEIASSGPEGWAARLTPFAGKL
                     VQWFAAQAGSVGMMAVQFLLTVMISAILYSNGETAAEGVRRFVRRLAGPRGEDALTLA
                     GKAVRGVALGIIVTALVQSILGGIGLAVAGVPAAGILTAVMFILCVAQVGAGVVLIPA
                     VIWLYWGGHNIWGTVLLVVSILVLSLDNFLRPMLIKKGADLPLVLILTGVIGGLIAFG
                     IIGIFIGPVVLAVTYRLLGAWVEGEMDEAGMEIGSKG"
     gene            8105..8491
                     /locus_tag="VEL68_22975"
     CDS             8105..8491
                     /locus_tag="VEL68_22975"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HXZ38911.1"
                     /translation="MQEKKRMIGPFLSVAAAFGFLLGGCASDGTLATQKISAGEKAVL
                     DAREGNASQNAAAELNAAEGNLSEAREAFAKKEYEKAARLADRASIDAELARNKATAA
                     KGKKNAEEMRKNIEALRQEIEGLSKK"
     gene            8613..9632
                     /locus_tag="VEL68_22980"
     CDS             8613..9632
                     /locus_tag="VEL68_22980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXZ38912.1"
                     /translation="MPDRFPKWVKIGLGFMALAGFLAAGCANSQQEKIAKDRLELART
                     AYLEAKANRNVEAFAPLPFAEAGKALQAAEQAKNAVEMEQLSYVAEKRAKIAVAVAEG
                     RMAERDADRLSKETADITAQKRTQEARIAQKEAENARLLAMAEAERATKAKKEAEEAR
                     SLAAAEAERAAKAKGEAEQARMAARAEAERAAKAKAEADQLTKELSDLKAKQTERGII
                     LTLGDVLFATGKADLSPEAMRSVDKLVEFLKKYTNRNVLIEGHTDSVGSDEFNLTLSQ
                     KRADSLKEALTGKGIEERRITTVGYGKKYPVASNDTAAGKQQNRRVEVSILNEGVKPE
                     TQFRQ"
     gene            9692..10159
                     /locus_tag="VEL68_22985"
     CDS             9692..10159
                     /locus_tag="VEL68_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38913.1"
                     /translation="MKWAIPALALSFFLFSAPLSAQVSAEFEKTREAMQKQKDDMIAA
                     DMKLTEEEGKAFWPLYQEYQEALRKLQDRSFKLLAEYAQERENETFTDQKAKALLDEY
                     MDIEREDLWLKRTYLERFNKRLPAKKVMRYFQLENKIAAMINYQTTQMIPLAK"
     gene            10329..11237
                     /locus_tag="VEL68_22990"
     CDS             10329..11237
                     /locus_tag="VEL68_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXZ38914.1"
                     /translation="MKGKEKRGEASHSGNREASRKKRELKEQVIRFCRDRGVDLIGFA
                     PVERWDEANEVPADFRPRALWPPAKTVIVLGLQMPLPIVETTPSVVHMELYKTANAEL
                     DQSAYDLARYLNRLGHASIFFPRDGYSSMRALRENNRAAFAHIPAAKYAGLGTIGASH
                     NLLTPEFGPRVRFVSVFTSAEIPPDPMLGKDLCIKCQACAKCCPKKAIRMRPDRVIGD
                     YDDQACLKMAEELVAQRCFPCGVCTKVCPIGEDRTLYKQSGSMKKYLREAEALAANPE
                     DPEYKSWSHLRKYGVARGKKQGRGEE"
     gene            11323..12039
                     /locus_tag="VEL68_22995"
     CDS             11323..12039
                     /locus_tag="VEL68_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38915.1"
                     /translation="MNLEDFCEGFQSLQRERPKTTRLLDAGQQLLGELLKDPTWFGEF
                     LQRYIAGPGFLTDQPTSVFDNEIRVHRSPEKAFTILAYIWDNPDHCPVHDHGAWGLIG
                     SLLSPLREVKYRRLDDGKVEGFAELEQVSDSLLEPGKVGFVLPLNKGIHQTGAAGHAL
                     TLSLGVYGRSIRQGYIHFFNPAEKKMIRAHTRILFRKVLALRALASLGEAVGQTFLTP
                     SLLESLPEDVAREFRRFSSS"
     gene            complement(12081..>12475)
                     /locus_tag="VEL68_23000"
     CDS             complement(12081..>12475)
                     /locus_tag="VEL68_23000"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXZ38916.1"
                     /translation="AIDRPKWKERLRPTAPQGVGLGFASGIISAMAHVGGLIFSFYLL
                     PHSRTKEVFVGTTVFLFFTTGLLKIGSYVYYHILTFHILMLCIALLPALFLGSISGKW
                     LNQRISNKLFIRLICVFVGLMGIWLVLT"
BASE COUNT         2804 a         3314 c         3798 g         2559 t
ORIGIN      
        1 aggcggtggt tcttccatgg gatcgggcct gtcctggggg gattgagacg gaaaaatgct
       61 ttggctcttt tttctgattg gaggaatctc ctctcaggcc tctctctgcg gaacatgggg
      121 aggggtttca tggaaggata agcacccggc gagcctgatg atccaaattc ctccaccctg
      181 aaccccaaca agcacgaaaa actgggggtg ggcaaggttg agatctcggt tgacacggac
      241 cggaaggggt tcctaagata ggaagtgagc cctcttctgt ttttcctgtc tttgggaccc
      301 catctgttta gctcttggag cggcctggcc aaattggtca tggggagcat cggacaatgg
      361 cagatcaaga cctaagcaaa ttgaccatcg ataaatccaa aacagccttt cggccggctc
      421 gtgggaaaaa ggtcctgtac ctggtttttc tcctcctcgc tttgcttacc gcggggttcc
      481 tctaccggat ggaaattttc actccaccca ttcaggttca gctggccaac gtaacccaaa
      541 tctacccgtc ccagggcttt acgatgctca cggccagcgg atacgttgtc ccccagcgaa
      601 aatcggccct agcctccaag gtgaccgggc gactggtctg gctgggagtg gaggagggga
      661 gccggatcaa gaagggagag gtggtggcgc gcctggaaag ccaggatgtg gaggcgacga
      721 aggcccaggc cgcagccaac ctggaaacgg ctcgtttcaa tctcgagaac gccagggcgg
      781 agctggtgga tgcgactctc aacctcaatc gaagcaagga gctggtggac aagaatttta
      841 tcgcccgtgg agactatgat acgaccgtgg cccggtacaa gaaggccgag gctgcggtca
      901 aggctgcgga ggcggtgatt cgagcgaacg aggcggctct ggaagcggct gccatccagc
      961 tggaatatac cctgatccgg gccccattcg atgccgtggt tctgaccaag aacgccgaca
     1021 tcggggacat cgtgaccccc ttcagcgcgt cggtggaatc caagtcggcg gtggtcacca
     1081 ttgccgacat ggattcgctc caggtccagg cagatgtttc cgaatcaaac ctcggccagg
     1141 tgaaaatcgg acagccatgc gaggttcagc tggatgcctt gccggatcgg aggctgaggg
     1201 gagccgtcca tatgatcgtg cccacgggag accggagcaa ggccgcggtc atggtgaaaa
     1261 tccgtttcat cgataaggac ccccgaaccc tgccggaaat gagcgccaag gtttcttttc
     1321 tttcccgaga gattaccaag aacgatcaaa ggccgagaac cgctgtgagt cagaaggcgc
     1381 tggttgcccg aggggacggg aagcatgtgt tccgggtaca ggggaatcga gcggtgcagg
     1441 catcggtcct tctggggaac ccgatcggcg atatggttga agtgatcgac gggttgaagg
     1501 caggagatca ggtggtcctg gacccgccgg ccaagctcaa aagcggagcg aagattaagg
     1561 cgggagagtg accattgcgg atttcggatt gcagattgaa cagctctgaa atgcggaatt
     1621 cggaatgccg aatggaaaaa attcggaaac cgatacagaa aagcaccaaa ttacgagcac
     1681 caaatcccaa ataaattcca atgatcaaaa ttccaaacct tggggcgcca gaatttcttg
     1741 tttggcattt ggagcttatt gggaatttgg aatttcgacc ttcccccggc aatgaaaggc
     1801 aagatcagtt gtgtctccca tcgttgaaat ccagaacttg agcaagtcct accgccgggg
     1861 ggaccggcgg gtgccggtgc ttcagaatat caacctggat attcaagaag gagaattcct
     1921 ggctctcatg gggccatccg gctccgggaa gagcaccctc ctgaacctga tcgccggcct
     1981 cgaccaggtg gatgacggaa cggtgatcgt agggggggtg aatatcacct ccctttcgga
     2041 aagcgagctg gctgcctggc gggccaccca cgtggggttc attttccagt tctacaatct
     2101 gattccagtt ctgactgctt tcgaaaatgt ggaacttcct ctccttctca ccggtttgtc
     2161 acggagggag aggagggagc atgtggagtt ggcccttcgg gtggtgaacc tggaggcccg
     2221 gatggatcat cgtccccccc agctttctgg aggggagcag caacgggtgg ccatcgcccg
     2281 ggccatcgtg actgacccga cgattctggt cgccgatgag cccacgggag acctggaccg
     2341 ggtctccgcc gaagaagtcc tcagcctgat ggagaggctg aatcgggagt ccaccaagac
     2401 cattatcatg gtcacccatg acccgcgggc agccaagaag gcggggatca cccggcacct
     2461 ggataagggg ctcttgaacg atgttcattc tcaagctcct gtttaagaat gccttccggc
     2521 acaagctgcg cacctcgctg accctcctca gcattgccat cgccatcatg gcctttggcc
     2581 tcctccgcac ggtggtcagc gcctggtatt caggggtgga agcctcctca gccacccggt
     2641 tgatcacccg gaactccata tccctcgtct ttccccttcc cctagcatac aaggatcgca
     2701 tccgccaggt ggagaacgtc accaccgtat cctttggaaa ctggttcggc gggatttaca
     2761 ttgaccggaa gaattttttc cccaattttg cggtggaccc taaaacctac ctggacctgt
     2821 accccgaact gattctctcc ccggagcagc gccaggcttt cctgcgcgac cggaaagggg
     2881 cggcggcggg gaagaagctc atcgaaaggt tcaagtggaa gatcggggat ttgatcaccc
     2941 tgcagggaac catcttcccg ggcaactggg aattcgtcct ccgggcgatt taccgaggaa
     3001 gggatgaaaa cgtggacgag agccagtttt tctttcactg ggactacttg aacgaagccc
     3061 tgaggaagac agccccctcg cgggccgacc gggtgggttt ttacatgatc ggagtggacc
     3121 acccgaacaa ttcctccaac gtggtccaag ccatcgaccg gaccttcaag aactccctgg
     3181 ccgagaccct caccgagacg gagaaagctt tcaccctttc ctttatctcc atgtcggagg
     3241 ccatcatcgt ggccatccag atcgtctctt tcgtggtcat cggaatcatc gtcgcagtgg
     3301 tggccaacac catgtccatg gcggcccggg aacggacctg ggagtacgcc gttctcaaga
     3361 ccctgggttt cgggggatgg catatcgccg gccttattct gggggaatcc ctgctgatca
     3421 ccctgatggg gtgcgcgctg gggatggcca ttacctttcc cggggcgtat atttttaaga
     3481 agtccgttcc catgtatctg ccggtcttcc aggtggagga aacgacttta tacctggacc
     3541 tggtggcctt tcttggagtg gggattctct ccgccctcat tcccgcctgg cgggcgattc
     3601 aggtgcgcat cgccgacggc ctccggagga tcgcctaaat gggactaccc ctatcctaca
     3661 gtatccgcaa cctctggacc cgccgcctga cgacaattct caccgccttc ggcatggccc
     3721 tggtggtttt tgtcttcacc gccatcctga tgatggccga gggcctgaag aagaccctgg
     3781 tggaaaccgg ctccccggat aacgtggtgg tcctgcgcaa aggctcctct tcggaagtgc
     3841 agagcggggt ggagcggatt caggcctcca tcatcgaaac ccagccggaa gcggctgtcg
     3901 gggccaacgg gaggctcctg ctggccaagg aggcggtggt gctcatcagc ctgcccaagc
     3961 gggaaaacag caaggccgtt tcgaacgtgg tggtccgggg gatcggggaa agttcattgg
     4021 cccttcgccc gcagatgagg ttggtccagg gccggatgcc ccggctcggt ttgccggagg
     4081 tcatggccgg ccagaagatc gcggagcgtt tccggggagg ggggctgggg gaaacgctcc
     4141 gcttcgggct gcgggactgg accgtggtgg gggtatttga cgcgggcaat acgggattca
     4201 gttcggagat ctggggggat gcggaccagc tgatgcaggc tttccgccga cgggtctatt
     4261 cctcggtcgt cttcaagctc catgacccgg gcgaatttct tcccttcaag cggcgcatcg
     4321 aaaccgaccc ccgcctgaac ctagaggcca agcaggagac gcggtattat gccgagcagt
     4381 cggagatcat gtccaagttt ttgaacatct tggggctttc cctcaccatc atcttctcct
     4441 tcggggccat gctgggggcc atgatcacca tgtactcctc ggtggccagt cggacgagcg
     4501 agatcgggac tctccgtgcc ctgggatttc agcgggggag catcctgcgg gcctttctct
     4561 acgagtctct cttccttggt ttgatcgggg ggggtgcggg gattttcctg ggttccttca
     4621 tgcagctgat caccatttcc accatgaact ttcagacttt ctccgagctg gccttctctt
     4681 tcaccctgac ttgggagatt gcctggaagg ccctggcctt ttccctgggc atgggtttct
     4741 tgggcggttt tctcccctcg atgcaggctt cccggatgaa catcgtggat tcccttcggg
     4801 cacagtgaaa gagaagaacc cccttttttc cctctgcatt actaaccttc aatattggta
     4861 atttctgttg agaaaaaatg taggggcggc cccctgtggc cgcccacagg atgggcaacc
     4921 acagggggtt gcccctacaa tggatgaatt attcttagcg gatagaggct ggggtatgat
     4981 caatttttct ttggttgcgg cgcagccgcc ctgtgcaatg gccagctatt ctttccagga
     5041 gcgggggcgg gtgttttcgg cgcagatctt gcgccgggca aagtggcgct gccgcagatt
     5101 gaagggcagg taggcaaggt agggatgcaa ggctggccag aaatccaccc gtttccagat
     5161 ggaagaaaaa ttataaaact ccttccaggc ccatttccag ccttcccgga gctgctcggg
     5221 ctccatgccc agagggcgaa aatgaggggc caggatttcc tgattgggaa gaagccacca
     5281 ttggtcacgc agaaggcgtc cctccgcctt gaggcgggaa tagaaacggg ttccggggta
     5341 gggggtcagc atggcaaatt gggccaggat aatcttacta gcgatggcga attccaccgt
     5401 gcgctcgaaa atcgaaggat cgtcaaaatc aaaaccgaac atgaaagccg cataggcaat
     5461 aatcccgtgg tcatggagct tatggatgat ctcccggtac tgctccggat ggttctgggt
     5521 cttgccggtg gttttcagct gggggctcag ggattcaaat ccgatgaaga gagccttaca
     5581 accgcttctt tgcaagattc tcaggttttg ttcctggtcc aaacccgcca gggaagcctc
     5641 tccgatccac cgcaatttca ggggctccag ggcggtaaag agcttctgga agtaagggac
     5701 gttgccgatg acattatcat cggcgaggag gattccccac cgcttcagct cccggacctc
     5761 ggcaatgact ttttcgatgt cgcgcatgcg gaaggggctg ccgaagattg agttgaccgt
     5821 gcagaagtcg caatgaaagg ggcagccccg ggtggtctga atgacatcca gcggaacata
     5881 gctgcgggcg tagggctgga agatcgtcct ccggggggcg ggagagcggt tcatgtccac
     5941 ccaccggtcc tgtcggtaga atttctgcaa gtggttggtt cgaaaatcct ccagaagccg
     6001 gggccagaga atttccgctt ccccgatcac caccgcatcg gcgtgggttg aagcctcttc
     6061 cggaaggaca gtgggatgaa taccccccag gaccacctga attccttttt ttcggcaggc
     6121 gtcggcgatc tcgtaagccc gatgagccgt cttggagagg acggaaatgg ctaccaggtc
     6181 tgttcccggg aacgcgttgc aaaggtcgat ggggctgagc tggtcgtcgg aaaaatgaac
     6241 ttccatatcg ggagggatca gcgcggccag agtggccagg gccagcgggg tcagacgggc
     6301 gctttcttcg gctgcggccg agacaagttc aattctcatc gttcaatcat aaggcaggga
     6361 aaggagcaga tcaacccatt ctttttcatc cccctttccg aatttctccg atgttttggc
     6421 ggtttacttc ccttgatccc tcgccttcct ttaaaaactt cctgccggat taaacatcct
     6481 tagggttcgc acggaaaggc cctaaaggct gtttcatata tcttttttca gctcaaaata
     6541 gtggggatag cggactttgg tttgaaagaa cggataaaag gatgctatgc agggaatcac
     6601 ctttgtgata gcctgaggcc gagaagtaaa ctggaaatgg atcaaattcc cctcccatcc
     6661 ttacagcagc gggggggaga ggatggaaaa atggaagaca agagtggaaa gttgcccgag
     6721 gcgaacagtc ctcccggacg agacctcacc cgcacgacgc tgggggtact gttgatcggg
     6781 attctcattg tgggaaactt ttggatcctg cggcctttcc tgccctcctt gttatgggcg
     6841 gtcatgatcg tcgtggctac ctggccgctc atgctgggat tacaggcgcg gctttggtgc
     6901 aagcgggggc ttgccgtggc agtgatgacg ttggccctgc tgttggtttt tgtcgtgccc
     6961 ttctcccttg ccgtggcaac catcgtggag aatgcggacc agatcgccga ctgggccaag
     7021 tctttgaaag agctggccct ggcggccccc cccgactggg tgggaaggat ccccctggcc
     7081 ggcccgaaaa tatcagccgc gtggcaagag attgcctctt ccggcccgga aggatgggcc
     7141 gcgcggctga cgccttttgc cggcaagctg gtgcagtggt tcgcagccca ggccggcagt
     7201 gtgggcatga tggcggtcca attcctcctc acggtgatga tctcggcgat cctgtactcc
     7261 aatggagaga cggccgccga gggagttcgc cgctttgtcc gccggcttgc cggaccccgg
     7321 ggtgaggacg ccttaaccct tgccgggaag gcggtccggg gcgtggctct cggtatcatc
     7381 gtcaccgccc tggtgcaatc gatcctcggg ggaattggcc tggcggtggc cggtgtgccg
     7441 gctgcgggga ttctgaccgc agtcatgttc atcctctgcg tagcccaagt gggggccggc
     7501 gtggttctga ttcctgcggt gatctggctc tactggggcg ggcacaacat ctgggggact
     7561 gtcctcctcg tggtgtcgat cctggttctg agcctggaca attttctgcg tcccatgctc
     7621 atcaaaaagg gcgccgacct gccgctggtc ctgatcttaa cgggagtaat cggcggtctc
     7681 atcgccttcg gaatcatcgg aatcttcatc ggtccggtgg tgctggcagt cacatatcgg
     7741 ctgctggggg cgtgggtcga aggggaaatg gatgaggccg ggatggagat cggcagcaag
     7801 ggttaaggga aattggattg tctccagcgg tccaagaggc aggacccgtt ccctccccat
     7861 ggaaaccgga ggatggtatt tggaaaagaa aagattttag gattggggag tgcggtcttt
     7921 ctcccgggga aaagagaatg gcaccaagaa tcgaaaaaac ttataatggc ttaataaact
     7981 ttttcagcat cccgggacga gggaggagaa aggtttagag ggtttcttca aaattcctcc
     8041 agcctaacgg ctgcccttgt acggaaacta ggatttcaaa gcggcccgaa aaggagaaga
     8101 agtgatgcaa gagaagaaga gaatgatcgg cccattttta tcggtcgcag cggcgttcgg
     8161 cttcctgctg gggggatgcg cttccgatgg caccctggca acccagaaga tatcggccgg
     8221 ggagaaggcc gtcctggatg cccgggaggg gaatgcttcc cagaacgccg ccgccgaact
     8281 gaatgccgcc gaagggaatt tgtccgaggc cagggaggct tttgccaaga aggaatacga
     8341 aaaggccgcc cgcctggcgg accgggcttc catcgatgcc gagttggcgc gcaataaagc
     8401 gaccgccgca aagggaaaga agaacgccga agagatgcgg aaaaacatcg aggccctgcg
     8461 ccaggagatc gaaggactgt cgaaaaagta attcttcccc cctttttcac cgctctccca
     8521 agaaagagag agagcgagga aaaggggctt cagactgcaa aatcaaaatc tcaagctgga
     8581 acgggtagga attcgaaagg aggggataga ccatgcccga tagatttccc aaatgggtaa
     8641 aaataggatt gggattcatg gccctggccg gtttcctggc cgccggatgc gccaactcgc
     8701 agcaggagaa gattgccaaa gaccgtctgg agttggcccg gaccgcctac cttgaggcta
     8761 aggccaaccg gaacgttgag gcctttgccc cgcttccctt cgccgaagcc ggcaaggccc
     8821 tccaggcagc ggagcaggcg aagaacgccg tagaaatgga gcagctgtcc tacgtcgcag
     8881 agaaaagagc gaagatcgcc gtggccgttg ccgaaggccg gatggccgag cgggacgctg
     8941 atcggctcag caaggaaacg gcggacatta ccgcccagaa gcggacccag gaggcgagaa
     9001 tcgcccagaa agaagcggaa aatgccaggc tactggccat ggcggaggca gagcgggcaa
     9061 ccaaggccaa aaaggaagcc gaggaggcga ggtcattggc cgcagcggag gcggagcggg
     9121 cggccaaggc caagggggag gcggaacagg cgagaatggc ggcccgggcg gaagcggagc
     9181 gggcggccaa ggccaaagct gaggcggatc agctcacgaa agaactttcc gacctcaagg
     9241 ccaaacagac cgaacggggg atcatcctca ccctggggga tgtcctgttc gccaccggca
     9301 aagccgacct atccccggaa gccatgcgca gcgtggataa actggtcgaa tttctgaaga
     9361 agtataccaa tcggaatgtg ctgatcgaag gccataccga cagcgtggga agcgacgaat
     9421 tcaacctgac cctttcccag aagcgggccg attcgctcaa agaagcgctg accggcaagg
     9481 ggatcgagga gagacggatt acgacggtag ggtatgggaa aaaataccct gtggctagca
     9541 atgataccgc ggcagggaaa caacagaacc ggcgggtgga agtgagcatc ctgaacgaag
     9601 gggtgaaacc cgagacccag ttccggcagt agagagcccg aggatcggtg gctttggcca
     9661 ggcaaggtat ttgcagaaag gggagggaag tatgaaatgg gcgattccag cattggccct
     9721 ttcatttttt ctgttttcgg caccgctgtc cgcccaggtt agcgcggaat tcgagaagac
     9781 ccgggaagcg atgcaaaaac agaaggatga tatgatcgcc gcagacatga agctgacgga
     9841 ggaggagggc aaagccttct ggcctctcta ccaggaatac caggaggccc tgcgcaaact
     9901 gcaggaccgg tccttcaagc tcctggccga gtacgcccag gaaagggaga atgaaacctt
     9961 cacggatcaa aaagccaaag ccctcctgga tgaatacatg gacatcgaaa gagaagacct
    10021 ttggttgaag agaacctacc tggaaaggtt caataagcga ttgccggcca agaaggtgat
    10081 gaggtatttc caactggaga acaaaatcgc ggccatgatc aattatcaga ctacccaaat
    10141 gatccctcta gccaaataat ttctttccat ccatcccaca ttttctttcc ctgtatcaag
    10201 agccgcttct cttccccctt cgggtccggg gggagaattt ctgggattgc agtggcggtc
    10261 gttcggtttg atttttaaca ggatgtcacc tatgctagat aaagggaaaa gttaaaaagg
    10321 aaatggctat gaaagggaaa gaaaaaaggg gggaggccag ccattccggg aatagagagg
    10381 cttcccgaaa gaagcgggaa ttaaaggaac aggtcatccg cttctgccgg gaccgggggg
    10441 ttgatctgat cggttttgcc cccgtagagc ggtgggatga agccaatgag gtgcctgccg
    10501 atttccgccc ccgggccctt tggccgccgg ccaagacggt gatcgtgttg ggcctgcaga
    10561 tgcccctgcc cattgtggag accactccct ccgtcgtgca tatggaactg tacaaaacag
    10621 ccaacgccga gctggatcaa tcagcctacg atctggccag atacctaaat cgcctagggc
    10681 atgcttcgat ctttttcccc cgcgatggct acagcagcat gagggctctc cgggagaaca
    10741 accgcgccgc ctttgcgcat ataccggcgg ccaagtatgc gggcctgggg actatcggag
    10801 ccagtcataa ccttctgacc ccggaattcg gcccgcgggt ccgctttgtt tccgtcttca
    10861 cttcggcgga aattcctcca gaccccatgc taggaaaaga cctttgcatc aaatgccagg
    10921 cctgcgccaa atgttgcccg aagaaggcca tccggatgcg acccgatcgg gtgatcgggg
    10981 attatgatga ccaggcctgc ctgaaaatgg ccgaggaact cgtcgctcag cggtgttttc
    11041 cctgcggggt ttgcaccaaa gtctgcccta tcggggaaga tcggacactc tacaagcaat
    11101 ccgggtccat gaaaaagtac cttcgagagg cagaagccct ggcggcgaat ccggaggacc
    11161 cggaatacaa atcctggtcc catctgcgga aatacggggt tgcgaggggt aaaaagcagg
    11221 gaaggggcga agaataaggg gcgtggatgg gcgagatcgc gcgagtcgca ttccggatcg
    11281 tctttgcagg gaggagatag gggcaatcaa ggagataaat ccatgaacct ggaagatttt
    11341 tgcgaaggtt ttcaatctct ccaaagagaa aggcctaaga ctactcgact cctcgacgct
    11401 gggcaacagc ttttggggga actgctgaaa gacccgacgt ggtttgggga atttttgcag
    11461 agatatattg ccggtcctgg cttcctaaca gatcagccca cctcggtttt cgacaacgaa
    11521 ataagagtcc atcgcagtcc ggaaaaggcc ttcaccatcc tggcctatat ttgggacaac
    11581 ccagaccact gtcccgttca tgaccacggc gcctgggggc tgattggctc ccttctgagt
    11641 cccttgcgag aagtgaaata ccgccgattg gacgatggga aggttgaagg gttcgccgaa
    11701 ttggaacagg tctcggattc tttgctggaa ccgggcaaag tcggattcgt tctccccctg
    11761 aataaaggaa ttcatcagac tggggcggcg gggcatgccc tgaccctctc tcttggggtt
    11821 tatgggagaa gcatccgaca gggttacatt cattttttca acccagcgga aaaaaagatg
    11881 attcgagccc acactcgaat tttgtttcga aaggttttgg ccctccgggc cttagcctcc
    11941 ttgggagagg ctgtggggca aacattcttg accccatccc tgcttgagtc cctgcctgaa
    12001 gatgtagccc gagaattccg gcggttttct tccagctgaa cttcggtttt tgcatcgggc
    12061 tctttgcgcc atacggttca ctaggtcagg accagccaaa tccccatcaa ccccacaaag
    12121 acgcagatca aacggataaa gagcttattg gaaatgcgct ggttgagcca cttcccggag
    12181 atggaaccca ggaagagagc gggcagaaga gcaatgcaga gcatgaggat gtggaaagtc
    12241 aaaatatggt aataaacgta agaaccgatc tttaacagcc ctgtggtgaa aaagaggaaa
    12301 accgtggtcc ccacgaagac ctccttcgtg cggctgtggg gaagcaggta gaaggaaaaa
    12361 ataaggcccc ccacgtgagc catggcgctg ataatgcccg aggcgaaccc cagccctact
    12421 ccctgcgggg cggtcgggcg gagacgctct ttccatttcg gccggtcgat ggcca
//
LOCUS       DASPRR010000627         8336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_300066, whole genome shotgun sequence.
ACCESSION   DASPRR010000627 DASPRR010000000
VERSION     DASPRR010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300066"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VEL68_23005"
     CDS             <1..343
                     /locus_tag="VEL68_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38917.1"
                     /translation="LRSLIVLVCILLGSLIGAVNFSGPLLVLFLAFLFVSSAGLNVTL
                     SAQANALSTQMERPHLYLGVYATAIDGGAAVGPLLAYSIGTLAGFGTLYLVAGSVLAL
                     AIFRYCWPGEK"
     gene            complement(642..908)
                     /locus_tag="VEL68_23010"
     CDS             complement(642..908)
                     /locus_tag="VEL68_23010"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXZ38918.1"
                     /translation="MTYRVEFKKSGKTLEWDSSAGNLLDFAEENGIQMECGRRMGVCG
                     SCKVKLISGTVAMETEEGLEDADKANRMILPCVAVPESDLVIEA"
     gene            complement(970..1731)
                     /locus_tag="VEL68_23015"
     CDS             complement(970..1731)
                     /locus_tag="VEL68_23015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HXZ38919.1"
                     /translation="MLLNDRVALVTGASRGIGASTAKILAQRGARVAMGARTVSDLQA
                     VAASIQKKGGKALVVPCDVVDPSQVRAMIEAVTKKWGRLDILVNNAGLGTQAKPVEEV
                     SPEEWDETILLNLKSVFLCTRAAAPVMKRQKYGRIVNVSSFAGRFFSRLSGPSYSSAK
                     AGVLGFTRHMAAELGPFGICVNSVAPSPTLTERVKVKWDSRPEEERQKILAGIPLGRV
                     AKPEEIATVIAFLASDDASYVNGVCIDVNGGSWMT"
     gene            complement(1747..2166)
                     /locus_tag="VEL68_23020"
     CDS             complement(1747..2166)
                     /locus_tag="VEL68_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HXZ38920.1"
                     /translation="MKIVLKCRKVVGGVAEGEAVVTKEPVSFLGGVNPDKGMVVERGH
                     EAEGQVITGKVFVFPHGKGSTAGPYIMYAMSKRKTAPSGMINVQAEPIIAAGAAIGNI
                     PLVHRLDQNPLEVIRTGDFVRIDADQGLVEVTRKEES"
     gene            complement(2163..3416)
                     /locus_tag="VEL68_23025"
     CDS             complement(2163..3416)
                     /locus_tag="VEL68_23025"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HXZ38921.1"
                     /translation="MNLNDREKKMLDGAFGAGPQTAMRLLVTLGEVYEAKRMIPVSSC
                     HVGGRSFLISGEESIEWMNELYDGGARFQVFTSTNPCSVDFDRWKEMGLPEDLVRKQR
                     RTDECYMKMGVVPLGSCLPYQTGNLPLPGSHFSWGGSAGATFSNSVLAARGNREGMPS
                     VIAAAIAGVTPEYGLHLKENRYGQVLVDLSGLDHSSLRLSDYSAIGSYVGRTLVSKNP
                     VIVGLPKAFSQDQIRFLISAMPTAGAISLCHLVGITPEAPTVEAALGGKPPEDRISVG
                     IEEMKSSFRKLTTTPKEDVDLVTFGCPHCSIPQIREIASLLEGKRISPNTRLWVATSG
                     HLRNLAQRMGYVEIIERAGGLVLADLCVAPGAPFHLVQGVKTVAINSARGAYFIPGAC
                     NVDVIFGDTKDCIQAAIDGKWRGSR"
     gene            complement(3426..4538)
                     /locus_tag="VEL68_23030"
     CDS             complement(3426..4538)
                     /locus_tag="VEL68_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38922.1"
                     /translation="MRNDEWRLLDLKLSSYAAATPMISTLLKLKSEEKIPNTLALITF
                     EKSSACLFYYNDPEKELDLEFCRRNDIEIGRRDTGGSPYWADPGTLLIFFWFNRKQVA
                     GFPDTISEGYRFLIGAAAHAISERFGIPAVYRPLNDMEVSGKKIAGHTVTFLGDTCRW
                     GCGPQVLKPRMDLMSRALKVPPEKYADKEAKTVEARVTNFEELLGRAPSFEEIKQTYV
                     SSLERAMNMKFQRGGFSPEEERLIEKQTAQIFSEEWLTAMTERRKFGDDLTAGWPRGE
                     HALKVPQGPLIRAIVLLDGGVIRNISLTGSIHCIPVQLVEEMEAGIRGLDGGAEVVTK
                     VIQDLFSRPGVQVAGCSAGHFVQAILSAVENAKKAK"
     gene            complement(4569..5885)
                     /locus_tag="VEL68_23035"
     CDS             complement(4569..5885)
                     /locus_tag="VEL68_23035"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXZ38923.1"
                     /translation="MEPWLLALVLFLSMFLVLALGLPVAFTLGGLSLAIGYFVWGGEA
                     GFYAFTLSSFGKLTEFTITALPLFIMMAAVLQHSGLADDMYEMVYRWMGGIKGGLAMG
                     TIIISAIFAAMVGISTVATATLGITSLPSMRKRGYDKKLCVGVICAGGALGILIPPSI
                     IFILYGVEAEVSIGKLFFGGVIPGILLTLIFIIYIGFRCGINPAYGPPLPPEERFTLA
                     QKVQSLKGVILPVLVIFLVLGVIYLGYATATEAAAIGLVGSLICAAIRRQLNLTTLKK
                     MFIMGIGVNAMVCWIMIGAVAYSRLVTTSGVGEWFCALITGLELNRWVVLSGMQVMFF
                     ILGCLLDPAGIILITIPIFIPIVRALGFDTLWFGVLFVMNMEMAYLTPPFGFNLFVMR
                     GVAPEDITMGDIYRAIWPFVGLQALGLILVMIWPDIGTWLPQRMIR"
     gene            complement(5887..6393)
                     /locus_tag="VEL68_23040"
     CDS             complement(5887..6393)
                     /locus_tag="VEL68_23040"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXZ38924.1"
                     /translation="MKTFEKAISTINVLMMWIAGIMTILMGLAVTYDVIMRNLFKAPA
                     IWAFDFSNFSCAAVAFLAGGYTLIQHRHVNVDVLYRNFIPRNKALANLSTSILFFLLC
                     GVLVWMGSITAFESFQSGATSGGGWDIPLFLPQLLVPIGGLLLGLQGVVHWVRDCRTV
                     LNREEKGT"
     gene            complement(6443..7510)
                     /locus_tag="VEL68_23045"
     CDS             complement(6443..7510)
                     /locus_tag="VEL68_23045"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXZ38925.1"
                     /translation="MKKLAAISAAVALIISLTIAGPTLAQQTFNWKLSCTYPPPQFNW
                     ESKAIANDLFAKKVADVTQGRVKIKVFYSNQLVPQTQALDALKKGVVDLVSATSYWPG
                     TIPETDFVWLPFWSKGEEHALHALRDTKIGKVLDDGYRQQGSQILFYWPVSIEGIMSK
                     KPVRAFEDVKGMKLRVSSAIWPTWYKDMGAAPINVAVAEQYEALMRGTLDATIYPIYT
                     IETYKFHEVCKYLTLPGFVDPMPCYTLVSTKAWNALPPDLQQTVQKVAMEIEKESVIG
                     SMKLTDSVLEFCGKRGVEIIHLNKAEFEKFRQSAMPLWEKFAAKSPACAELVRIIKAD
                     LEEWMATRPEAKKWEERWLAK"
     gene            complement(7972..>8336)
                     /locus_tag="VEL68_23050"
     CDS             complement(7972..>8336)
                     /locus_tag="VEL68_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXZ38926.1"
                     /translation="EDLQPGMKLDGIVTNVTAFGAFVDIGVHQDGLVHISQLSDRFVK
                     DPHQVVKVQQKVKVTVLSVDLERKRISLSMKKEPGKPEREPKLEGKSSAGKPTAAGKK
                     SEPEQFNNPFVKAFRKEK"
BASE COUNT         1782 a         2449 c         2297 g         1808 t
ORIGIN      
        1 gcttcggtcc ctgatcgtgc tggtatgcat cctgctgggc agcctgatcg gagcggtgaa
       61 tttcagcggg cctcttttgg ttctttttct ggccttcttg tttgtgagca gcgccgggct
      121 gaacgtgacc ttgagcgccc aggccaacgc cctctccacg cagatggaaa ggcctcatct
      181 ttatctggga gtttacgcca cggccatcga cgggggagcg gctgtggggc cgctcctggc
      241 ctattccatc gggactctgg cgggattcgg gactctctat ttggtggccg gcagcgtatt
      301 ggccttggcc attttccggt actgctggcc gggggaaaaa tagacgcaaa tgtcaattag
      361 tgtccacccg gaaactccct cttccctcgg agactgtgtc gtaatccgtt catggttcga
      421 caagctcacc acgaacggat tatgagcgca ttaaaattta gccacttagc cgttcgccct
      481 gagcctgtcg aagggcgagc gggggattac gacacagttt cttgccggag aggggtgggc
      541 gagggaatcg agggggaagg aattattagg atttccagac aagcagcggg cacctaccat
      601 cctgtaaggg gtaaccagca accagtaaca agtaacgagg atcaggcctc gatcaccagg
      661 tccgattcgg gaacggcgac gcagggcaaa atcatccgat tggccttatc cgcgtcttcc
      721 agcccttctt ccgtctccat agcaacggtt cccgaaatca gtttcacctt gcaggagccg
      781 caaactccca tacggcggcc gcactccatc tgaatcccgt tctcctcggc aaaatcgaga
      841 aggttcccag cgctgctgtc ccactccagg gttttgcctg atttcttgaa ttccactctg
      901 taagtcacct tggtttctcc ttgcacccgg gcccccacat ggggaaaaga ctgtttttcc
      961 gccctttcct caggtcatcc agcttccccc attcacgtct atgcacaccc cgttgacgta
     1021 actggcgtcg tccgaggcta gaaaggcgat cactgtggcg atctcctcgg gcttagccac
     1081 ccgccccaga ggaattcccg ccaggatttt ctgccgttcc tcctcggggc gggaatccca
     1141 tttgactttc acccgctcgg tcagagttgg actgggcgcc acggagttga cgcaaatccc
     1201 gaaaggcccg agctccgcgg ccatgtgccg ggtgaacccc aacaccccgg ccttggcgga
     1261 gctgtatgag ggtcccgaga gccggctgaa aaaccggccg gcgaatgaag agacgttgac
     1321 gatacggccg tatttctgcc gtttcatgac cggagcagcg gcgcgagtgc acaggaagac
     1381 cgatttcagg ttcaggagaa tggtttcatc ccactcttcc ggggagacct cttccaccgg
     1441 cttggcctgg gttcccagcc ccgcattgtt caccaggatg tccagtcggc cccatttctt
     1501 cgttacagct tcgatcatgg ccctaacctg ggaagggtcc accacgtcac agggaaccac
     1561 cagggccttc cccccttttt tttgaatcga ggcggcgacc gcttgcaggt cggaaacggt
     1621 ccgggcaccc atcgccaccc gcgcgccgcg ctgagccagg attttggccg tggaagcgcc
     1681 gattccgcgg gatgcccccg tgaccaaggc aactcggtcg tttaaaagca ttcagaactc
     1741 ctgtctttaa ctctcttctt tccgggtcac ttctacaagg ccttggtccg cgtcgatccg
     1801 gacaaagtcg ccggtcctga tgacctccag ggggttttga tccagccgat ggaccagggg
     1861 gatgttcccg atggccgccc cggcggcgat aatgggctcg gcctggacat tgatcatccc
     1921 gctgggagcg gtcttccgtt tggacatggc gtacatgatg taaggtccgg ccgtggaccc
     1981 tttgccgtga ggaaagacga agaccttccc ggtgatcacc tgcccttcgg cctcgtgtcc
     2041 ccgctctacc accattccct tgtccgggtt gacccctccc aggaaactga caggctcttt
     2101 ggtgaccact gcctctccct cggccacgcc gcccacgact ttccggcact tcaggacgat
     2161 tttcatctcg accccctcca ctttccgtcg atagctgcct gaatgcaatc cttcgtatct
     2221 ccaaagatga cgtccacgtt gcaggccccg ggaatgaagt aggctccccg ggcgctgttg
     2281 atggccaccg tcttcacccc ctgcaccaga tggaaggggg cgcccggggc cacgcagagg
     2341 tccgcgagca ccaacccgcc ggcgcgctcg atgatctcca cgtaccccat gcgctgggcc
     2401 aggttcctca ggtgcccgga agtcgccacc cacaggcggg tattcgggct gattcttttg
     2461 ccctccagga gggaagcaat ctcccgaatt tgggggatgc tgcagtgagg acaaccgaag
     2521 gtcaccagat ccacgtcttc ttttggcgtg gtggtcagct tgcggaaaga actcttcatc
     2581 tcctcgattc ccacggaaat cctgtcttct gggggctttc cccctagggc ggcctccaca
     2641 gtgggagcct cgggggtgat tcccaccaga tggcagagag agatagcgcc ggccgtgggc
     2701 atggcggaga tgaggaagcg gatttgatcc tggctgaagg ctttgggaag gccgacaatg
     2761 accgggttct tgctgaccag tgtccgccca acgtaggaac cgatggccga gtaatcggag
     2821 agtctcaggg aagaatggtc caggccggag aggtccacca ggacctggcc gtaccgattc
     2881 tctttgaggt gcaggccgta ttccggggtc acgccggcaa tggctgcggc gatgaccgag
     2941 ggcatgcctt cgcggttccc ccgcgcggcc aggacggagt tggaaaaagt ggcccccgcc
     3001 gagcctcccc aggaaaagtg actcccgggg aggggaaggt ttccggtctg atagggaagg
     3061 cagctcccca gcggcaccac gcccattttc atgtagcact catccgtccg gcgctgcttg
     3121 cggacgaggt cttcaggcag gcccatttct ttccaccggt cgaagtccac ggaacacgga
     3181 ttggtggaag tgaagacctg gaagcgggcc cctccatcat agagctcgtt catccactcg
     3241 atgctctctt ccccggagat aagaaagctc cgccccccca cgtgacagga ggatacgggg
     3301 atcatccgtt tggcctcgta aacctctccc agggttacca gaaggcgcat agcggtctgc
     3361 gggccggcgc caaaggcgcc gtccaacatc ttcttttcac ggtcgttcag attcatagga
     3421 aaacctcatt tcgctttttt agcattctcc acggctgaaa ggatcgcctg aacaaagtgc
     3481 cccgcagaac agcccgcgac ctgaactccg gggcgactga ataagtcctg gatcactttg
     3541 gttacgacct ccgcccctcc atcgagtccc cgaatcccgg cctccatctc ttccaccagc
     3601 tggaccggaa tgcaatgaat cgagccagtc aaggagatgt ttcggatgac ccctccgtcc
     3661 aggaggacaa tcgcccggat caaaggccct tgggggacct tgagtgcgtg ctctcccctg
     3721 ggccatccgg cggtcaggtc gtcgccgaat ttccgccttt cggtcatggc cgttagccat
     3781 tcttcggaaa atatctgggc ggtttgtttt tctatgagcc gttcctcttc cggggaaaac
     3841 cccccccgct ggaatttcat attcatggcc ctttccaggg acgaaacgta ggtctgcttg
     3901 atctcctcga aggagggggc ccggcccagg agttcttcga agttggtcac ccgcgcctcc
     3961 acggtcttgg cttccttatc cgcgtatttt tccggaggga ccttcagggc ccgggacatc
     4021 aaatccatgc gcggcttcag aacttgtggg ccgcatcccc agcggcaggt gtcccctaag
     4081 aaagtcaccg tgtggccggc gatctttttc cccgacacct ccatatcgtt cagggggcgg
     4141 tacactgccg gaatcccgaa ccgctcggaa atcgcgtgcg ccgccgctcc gatcaggaaa
     4201 cggtatcctt cggaaatcgt atccggaaaa ccggcgacct gcttccggtt gaaccagaaa
     4261 aagatgagga gagtccccgg gtctgcccag taaggagaac ctcccgtgtc acgccggccg
     4321 atctcgatat cattacgccg gcagaattcc aaatcgagtt ccttctccgg atcattgtag
     4381 tagaaaaggc aggccgagga cttctcgaaa gtgataaggg ccagggtgtt cgggatcttt
     4441 tcctctgatt ttaatttcag gagggtgctt atcatgggtg tggcagcggc atagctggag
     4501 agcttcaaat cgagcagtcg ccattcatcg ttgcgcattc cgaatcctcc ctcattccag
     4561 agacacgatc aacggatcat cctctgaggc agccaggtgc cgatgtccgg ccatatcata
     4621 accagaatca atcccagagc ctgcaggcca acaaatggcc agatggcccg ataaatgtcc
     4681 cccatggtga tatcttccgg ggccactccc cgcatgacaa agaggttgaa tccaaaaggt
     4741 ggcgtgagat aggccatctc catgttcatc acaaaaagaa ccccgaacca tagggtatcg
     4801 aagcccagcg ctctcacgat ggggatgaag atggggatgg tgatgagaat gatccctgcc
     4861 gggtccaata agcatcccag gatgaagaac atgacctgca ttccggaaag gaccacccag
     4921 cggtttaatt ccagccccgt gatcagggcg caaaaccact cccccacgcc ggaagtggtg
     4981 accagcctgg agtaggccac ggccccgatc atgatccagc agaccatggc gttgaccccg
     5041 atgcccatga tgaacatctt cttcagggta gtgagattca gttgtcttcg gatcgcagcg
     5101 cagatcaggg atcccaccag cccgatcgcc gcagcctcgg tagccgtggc atatcccagg
     5161 tagatgaccc ctaggaccag aaagatgacc agaaccggca gaatgacgcc cttcagggat
     5221 tgcactttct gagccagggt gaaacgctct tcgggaggca agggggggcc ataggcggga
     5281 ttgatcccgc agcggaagcc gatgtagatg ataaaaatca aggtcaggag aatccccggg
     5341 attactcccc cgaaaaataa cttgccgatg gaaacttccg cctccacccc gtataggata
     5401 aatatgatgc tcgggggaat cagaatcccc agggctccgc cggcacagat gacccccacg
     5461 cataattttt tatcgtatcc ccttttcctc atagaaggta aagaagtgat ccccagtgta
     5521 gccgtggcca ccgtgctgat ccccaccatc gcggcaaaga tggcggagat gatgatcgta
     5581 cccatggcca gcccgccctt gatccccccc atccagcggt agaccatttc gtacatgtca
     5641 tcggccagcc cgctgtgctg cagcaccgcg gccatcatga tgaagagagg cagggcggtg
     5701 atggtgaact cggtgagctt gccgaaagaa cttagagtaa aggcatagaa ccccgcctcc
     5761 cctccccaca cgaaatagcc gatggctaag gataaacctc ccagggtgaa agcgaccgga
     5821 agacccaggg ccaggaccag aaacatggaa aggaaaagga caagggccag taaccagggc
     5881 tccatcctaa gttcctttct cttctctgtt caggaccgtg cggcaatcgc ggacccagtg
     5941 aaccactccc tgaaggccga ggagaagtcc accgatgggg acgaggagct ggggaaggaa
     6001 aagaggaata tcccagcctc cccccgacgt ggccccggat tggaaagatt cgaaggccgt
     6061 aatggagccc atccagacta aaaccccgca gaggagaaaa aacaggatgg aagtgctaag
     6121 attggccaga gctttattcc gcgggatgaa gttgcggtag aggacatcca cattcacgtg
     6181 ccgatgttga attaatgtat acccccctgc cagaaaggcc actgcggcgc aggaaaaatt
     6241 cgaaaagtcg aacgcccaaa tggccggagc cttgaacagg ttgcgcatga taacgtcgta
     6301 ggtaaccgcc agtcccatga gaatggtcat gatcccggcg atccacatca tcaagacatt
     6361 tatcgtagat atggctttct cgaacgtctt catcggtttg atttcccttt tcttctatcg
     6421 tccgggtacc cctcaacttc ggttacttgg ccagccatct ctcttcccat tttttcgctt
     6481 ccggccgggt cgccatccac tcttccaaat cggctttgat gattctgacc agctcggcgc
     6541 aagccgggga cttggctgca aacttctccc agaggggcat ggccgactgt cggaactttt
     6601 cgaactccgc tttgttcaga tgaatgatct ccactccccg cttgccgcag aattccagca
     6661 ccgaatcggt gagtttcatg gacccgataa ccgattcctt ctcgatttcc atggcaactt
     6721 tctggacggt ctgctgaagg tccggaggta aggcattcca agctttggtg ctaaccagcg
     6781 tgtagcaggg catggggtcg acgaagccgg gcagggtcag gtatttacag acctcgtgaa
     6841 acttataggt ctcgatggtg tatatggggt agatggtggc atccagcgtt ccccgcatga
     6901 gcgcttcgta ctgttctgcg acggcgacgt ttatgggggc ggctcccatg tctttgtacc
     6961 aggtgggcca gatggccgag cttacccgta acttcattcc ttttacatcc tcaaaagccc
     7021 ggaccggctt cttgctcatg ataccctcga tgctcaccgg ccagtagaag agaatctggg
     7081 aaccctgctg ccggtacccg tcgtccagga ccttcccgat tttcgtatcc cgcaacgcat
     7141 gaagggcgtg ctcctccccc ttgctccaga aggggagcca gacaaagtcc gtctccggaa
     7201 tggttcccgg ccaataggaa gtggccgaaa ccaggtcgac aacccctttc ttcagggcgt
     7261 ccaaagcctg cgtttggggc acaagctggt tgctgtaaaa gaccttgatc ttgacacgcc
     7321 cctgggtgac gtcagcgacc ttcttggcga agaggtcgtt ggctatggct ttcgattccc
     7381 aattgaattg agggggaggg taagtgcagc tcagcttcca attgaaggtc tgctgagcga
     7441 gggtaggacc agcaatggtg agactgatga tcagggcgac agcagcactg attgcggcga
     7501 gctttttcat ttccatcctc ctcttcagca attgtcccca aaggatttct catccggggg
     7561 aacgggtttc aggcttcggc aaagaacacc cagaccaggc cttgcctgcc tttcattgga
     7621 agagccatgg aagtaaagaa tcccgtctct ggcccagaaa cgggatttag gagagttaat
     7681 tcaatcgttt cctccggtga gtcagatgaa tggaacgatt cgtcaaaatt ccggtatgaa
     7741 caaaacgagg gtccccttga actggggtga atctaacaca aagccatttt tcgaatcaag
     7801 gaaattgttg atgaactcgt gaaaagtcaa aattccatga aattggtcat tccggcgaaa
     7861 gccggaatgc agctatttaa ataaattctg gaccccggct ttcgccgggg tgacgcttga
     7921 agagacttat tgcgaaatca tcgaattctt gctcgtccaa aaactgagga ttcatttttc
     7981 tttccgaaaa gccttcacga aggggttgtt aaattgctcg ggctcacttt tcttgcccgc
     8041 tgcggtaggt ttcccggcgg aagatttccc ttccagtttt ggctctctct ccggtttccc
     8101 cggctctttc ttcatcgaaa gggaaattcg tttcctctcc aggtctacgg agaggaccgt
     8161 gaccttgacc ttctgctgaa ctttgaccac ctggtggggg tcttttacga aacgatcgga
     8221 aagctggctg atgtggacca gcccgtcctg gtgcacgccg atgtccacga aggcgccgaa
     8281 ggcggtcacg ttggtgacga tcccgtccag cttcatgccc ggctggaggt cttcga
//
LOCUS       DASPRR010000628         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_155325, whole genome shotgun sequence.
ACCESSION   DASPRR010000628 DASPRR010000000
VERSION     DASPRR010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155325"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VEL68_23055"
     CDS             complement(<1..360)
                     /locus_tag="VEL68_23055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXZ38927.1"
                     /translation="MVGQQAAAQGWRRVGLHLFLGFTSFVMVAPFIWVLLTSFKRSTD
                     IVTETPQLLPQVWTLENYLKLPQVAPFVRFFLNSIIVSGVSTLFIALGSAACGYIFAK
                     YRFRGRDFLFFLVISTIL"
     gene            complement(373..1305)
                     /locus_tag="VEL68_23060"
     CDS             complement(373..1305)
                     /locus_tag="VEL68_23060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXZ38928.1"
                     /translation="MIQKAGKRSRVAVGVGGRLTRSQIGFLWGMVMPVIALFIIIRVI
                     PILVVLLMSFTNFNLNRPLTRFLPYQNFTRLVGDPNFLTAFINSTEFVIIAVPLEIIL
                     GLAFAIYLNRKVRFESVYETLYFLPYIVPMVPSAIIWKWIYAPGTYGLANYLLDQMGF
                     ARVGWMSTPQISLLAVIGIHVWKALGFFVIVFLVGLKNIPSDFREAAIVDGATSWEVT
                     RKVDIPLLKPVILFGVVMATIWAWSAFTEVYIISQGTDISTGAEIQVLVTRIYAEAFL
                     YFNMGYASAISFVLFIVSLILVALQFRLIRVESN"
     gene            complement(1553..>2282)
                     /locus_tag="VEL68_23065"
     CDS             complement(1553..>2282)
                     /locus_tag="VEL68_23065"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXZ38929.1"
                     /translation="SGGGFGTSQKYWCQVMIPYGAKVLEKVGNKWKAGYDNEPGRKAL
                     QYYIDAVFKYKVDSLDFKSDAEAFGLGITSMFQRESAVVGYMAKNAPKINYGVFLMPK
                     GPGGWGTVGNTMGLSISKSSKYKKEAFDFAMFMMNDDMSRATFGDSGWQPFRKGVDYS
                     ALYKSRPQLKGFIDGAQTPGHTVYDYENIAPIFEIHSRMADRLMAAFKRADLMDNPSG
                     IAKVIKDTAEETNRILEDHGLLGK"
BASE COUNT          532 a          635 c          666 g          449 t
ORIGIN      
        1 caggatggtg gaaatgacca ggaagaaaag aaaatccctt ccgcggaaac ggtacttggc
       61 aaagatatag ccgcaggcgg cggaacccag ggcgataaaa agggttgaga cgccagagac
      121 gatgatactg ttcaagaaaa atcgcacgaa aggcgccacc tgggggagct ttagataatt
      181 ctccagagtc cacacctgag ggagaagctg gggagtctcg gtcacgatat ccgtagaccg
      241 cttgaaggag gtgaggagca cccagatgaa gggagccacc atgacaaagc tagtgaaccc
      301 cagaaacaaa tggagcccga cccgtctcca tccttgcgct gcagcttgct gtccaaccat
      361 ataatattcc cgctagttcg actccacccg gatcagccgg aactgtaaag ccaccaagat
      421 caaggaaaca atgaacagga caaacgagat ggccgaggca tagcccatgt tgaaatagag
      481 gaatgcttcg gcgtagatcc gggtcaccag gacttgaatc tccgccccgg tggagatgtc
      541 cgtcccctgg gatatgatgt acacttcggt gaaggcggac caggcccaga tcgtggccat
      601 gaccactccg aagaggatga ccggcttgag gaggggaatg tccaccttcc gggtcacctc
      661 ccaggaagtc gccccgtcga cgatggccgc ctcccggaag tcgctgggga tgttcttgag
      721 gcccaccagg aacacgatca cgaaaaaccc cagcgccttc cagacgtgga ttccgatgac
      781 tgccagcagg ctgatctggg gcgtgctcat ccatcccacc cgcgcaaaac ccatctggtc
      841 caggaggtaa ttggcgaggc cataggttcc gggggcgtag atccacttcc agatgatggc
      901 cgaggggacc atgggaacga tgtagggcag aaaatacagg gtttcataga ccgattcaaa
      961 ccggaccttc cggttgaggt agatggcaaa ggccagcccc aggatgatct ccagggggac
     1021 cgcaatgatc acgaattccg tggaattgat gaaggcggtc aggaagttcg gatcgccgac
     1081 caggcgggtg aaattctggt agggaagaaa ccgggtcaag ggccggttca ggttgaagtt
     1141 ggtgaaagac atgaggagca cgactaggat ggggatcacc cggatgatga tgaaaagggc
     1201 gatcacgggc atcaccatcc cccaaagaaa cccgatctgg ctcctcgtca ggcggccgcc
     1261 cacgcccacg gccacccggc tgcgctttcc tgctttttga atcattctcc gctctgtgcc
     1321 accaaaatgc aaggtttggg aattattttc gagaggagat ggattggaaa cccggcctcc
     1381 taaatccccc ccaacccccc tttattaaag ggggggaggg gggatttctg gcatcccgtg
     1441 ggcccctggg agagtgggtg agccgggaac caggacccgc ttcctccacc ggccctcccc
     1501 ttccgctgga gggagaggat aatgcctagg aaacattcga tccagccggc gctcatttgc
     1561 ccagcagtcc atgatcctcc aggattcggt tggtctcctc agccgtgtcc ttgatgacct
     1621 tagcgatccc ggaggggttg tccatcaggt cggcccgctt gaaagcggcc atcaggcggt
     1681 cggccatgcg ggagtggatt tcgaagatag gggcgatgtt ttcgtaatca taaacggtgt
     1741 gccctggggt ctgggcgccg tcgatgaaac ccttgagctg cggccggctc ttgtagaggg
     1801 cgctgtagtc cacgcctttt cggaagggtt gccatccgct gtccccgaag gtagcccggg
     1861 acatgtcgtc gttcatcatg aacatggcga agtcgaaggc ttctttctta tacttgcttg
     1921 acttggatat ggagagcccc atggtgttgc ccaccgttcc ccatcctcca gggcccttgg
     1981 gcatcaggaa cactccataa ttgatcttgg gggcattctt ggccatgtag ccgacgaccg
     2041 cggactcccg ctggaacatg gaggtgatcc ccaggccgaa ggcctcggca tcggacttga
     2101 agtccaggct gtcaaccttg tacttgaaaa ccgcatcgat gtaatactga agcgcctttc
     2161 gtccgggctc attgtcataa ccggccttcc acttatttcc cactttttct aataccttgg
     2221 ccccgtaggg gatcatgacc tggcaccaat acttctggga agtcccaaac cctcccccgg
     2281 ag
//
LOCUS       DASPRR010000629         9094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1440757, whole genome shotgun sequence.
ACCESSION   DASPRR010000629 DASPRR010000000
VERSION     DASPRR010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440757"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VEL68_23070"
     CDS             <1..1104
                     /locus_tag="VEL68_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HXZ38930.1"
                     /translation="KWKKYGKDILPLWVADMDFVSAEPILRALHQRVDHAFFGYTIPL
                     GELCGTILNRLKALYQWEVREDEVFYLPGLVTGLNFAVQAFSVQGEGVLVQPPVYHHF
                     VKDPVHHGRVLVDPPLVENGDRYEVDFESFEKAITPQTKIFILCNPHNPVGRVFTRAE
                     LEKMAEVCLRHRVVICSDEIHCDLVFPEFRHIPIASLSPEVARWTITLMAPSKTYNLA
                     GLRCGFAVIQNPELAKGWRKATLGLNPGLNIMGQVAALAGYREGKEWLDQVLAYLRTN
                     RDFLHRTIPEKLPGVRMTKMEATYLAWLDCRKTGIPGNPSQFFLKEAKVALNDGGEFG
                     RGGEGFVRLNFACPQEMLNQALEQMSWAMQKLP"
     gene            complement(1483..3039)
                     /locus_tag="VEL68_23075"
     CDS             complement(1483..3039)
                     /locus_tag="VEL68_23075"
                     /inference="COORDINATES: protein motif:HMM:NF023299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXZ38931.1"
                     /translation="MTMNADKTKLKKIGVDELKVGMFIVNLGRSWISHPFFRNQLKID
                     SPKKIGKLKKYGIQEVYIDPQQGLDVPSIKVEGGGAIPSGDEGSNPGPNLTDKRPEEV
                     KALPSEAPSPPSQPVEAYSKKVTELVPTLEAAPSHPYKGGAVDEPKEEVPFERMEKSD
                     SVAFWEEKIGQVGESLFSPESPLRIPFDDEIPFIKEIHGARKVKAEAQTVVRNVINEV
                     RMGKSIEGDRVRRAVNSMVDSVLRNHEALASLARIRIYDEYIFVHSLDVCILSLSMAR
                     HLNLPREDMVEIGIGALLHDIGKLRIDPGILKKPDTLSEKEWVEVRKHPVYSLEIMEE
                     SKGIPEPSKQLALQHHERYNGNGYPFGLKGEAISLGGQIAGIIDFYDAVTTDRPFQKA
                     VQPHEAIRKLYEEGPVEFNRLMVERFIQCIGIYPFGTFVLLDTEEMGVVCGVKPEMLL
                     RPNVLIIYQNSKTLYPQPFLADLNEKADQSSWYKRSIIMPLEPEKWKLPVDDYLGEFK
                     RILREKGPSI"
     gene            complement(3311..4507)
                     /gene="hemW"
                     /locus_tag="VEL68_23080"
     CDS             complement(3311..4507)
                     /gene="hemW"
                     /locus_tag="VEL68_23080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXZ38932.1"
                     /translation="MMTEAKQFPNPKGSIRNPQFEIRNQGPPGLYIHIPFCVTKCPYC
                     SFYSQTDLSLIPGFIEALGKEMEMVSQTFSSPFDTVYIGGGTPSVLSPRQIEEILGKT
                     RKYFPLARTTEFTLEANPGDLDFSFLQALRELGIHRLNLGVQSFAPDVLFFLGRRHDA
                     EKAIQAIEASREAGFHHLGLDFMYGVPGQTLESWLDTLRRAITFSPEHVSCYQLTLEE
                     DTPMGKAHCRGTFAFPKEEDLRQFFLATSESLEKVGFIHYEVSNFSRGLQNASRHNQK
                     YWNHTPYLGLGPSSHSFSGSVRWWNTPSITAYMNTLRAGTLPLDNFEILTPEQLQTEA
                     FFLGFRTARGINLNDFSKNHGENFLQERRKILQKLEEEGLIVVQEGYLRPTREGLAVA
                     DRLALI"
     gene            complement(4497..6296)
                     /gene="lepA"
                     /locus_tag="VEL68_23085"
     CDS             complement(4497..6296)
                     /gene="lepA"
                     /locus_tag="VEL68_23085"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXZ38933.1"
                     /translation="MPENIRNFSIIAHIDHGKSTLADRFIQYTGLVEERNFRDQILDN
                     MDIERERGITIKSQAVTLPYRAKDGKDYLLNLIDTPGHVDFTYEVSRALASCEGVLLL
                     IDAAQGVQAQTIANLLLAMENNLEIIPVINKIDLPTADVDRVLEQIDTELGLDPATHL
                     KCSAKDGIGIEEILEAIVQKIPPPRGSPESPLAALIFDAQYDSFRGTIVHCRVFEGAV
                     KKGDTIRFLSNQADYEVEEVGRFSLCRERMEQLAAGEVGYIIAGIKTISDVRIGDTIT
                     LDSRSCGRPLPGFKDIKPVVFSSIYPIASDDYEDLSVALEKYKLNDAAFIYQKDSSVA
                     LGQGFRCGFLGLLHLEIVQERLEREYNLSIILSVPSVRYRFNLKDGSMVDVDNPAHYP
                     NRANVIKAEEPYIRATMIAPDKYLGGVMKLCTDRRGVNSKMNYLGPKRVELTYEMPLA
                     EVIYDFYDRFKTITQGYGSFDYDLLDYRESDLVLLDILVNGDKVDALSQVLHRGHARS
                     RALQACERLKEEIPRQVFRVAIQGAIGGEIIARTTISPYRKDVTAKCYGGDITRKRKL
                     LEKQKEGKRRMKMIGSVTIPQSAFLAVLKSNDD"
     gene            complement(6548..7858)
                     /locus_tag="VEL68_23090"
     CDS             complement(6548..7858)
                     /locus_tag="VEL68_23090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXZ38934.1"
                     /translation="MGEKVLQEVRKIWPMVQDPAPREYIARIGKRVLATMEAQPFEYE
                     FYVLNTPEINAFAVPGGKVFFNSGLILMVEKEDELAGVMAHEIGHVLARHIARQSEQG
                     MKLGLATLGALLAGIFLGPQAATAIGVTAQAASATAMLKYSREYEEEADYLGLKYMER
                     AGYDPQAMVTMLKKLRRISGPASTDPPAYLLTHPAAEQRMGNMEIQMMNLPKTENRRP
                     PEGNLKRVQTKLVCEEKDTARAVTYFENCRKRQPDDPECLFGLGLAQRKMGALDRSID
                     NLSQAASLAPQDGEIYRELAAAYFLKANVAEAQKYAEQARSLSPSDAKAYFYLGRVYL
                     EQKRVDDALQAFLTANKLDPNFGENYYHLAMAYGAKNMLGAAYRSLGYYYKLLGDGKT
                     ALAQFQRALPYYNDSPRERASIQKEVQELTPSKKELPPSKKDSK"
     gene            complement(8144..>9094)
                     /locus_tag="VEL68_23095"
     CDS             complement(8144..>9094)
                     /locus_tag="VEL68_23095"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXZ38935.1"
                     /translation="EGLGEAMADIQGYFWETADQVWQFAKEQSPKILGLPVEKARDSI
                     SRHAKEMPCSVTPFLTALEMLNPKSLLAPPSQLLSVPMLGILQATDPEGIRMEIEDFI
                     SRGYDTIKIKVGFDVDRDIERVKLAQEMIQGRAHLRADANQGYTLLHAKRFVQSIDPH
                     GIEFLEQPFKENDWDSMVELSKVSPLPLGLDESIYGMDSVEKAKKLGCARFVKFKIMK
                     MGSAEALAHAIEISRRMGFGIILGNGAAGEVSCYQEALVAGRMSIHAGEMNGFLKQKG
                     SILAEGLKIAGGKILLAPDYRLRLDSLKVDQFAVDRLILE"
BASE COUNT         1977 a         2338 c         2526 g         2253 t
ORIGIN      
        1 aaatggaaga aatatgggaa ggatatcttg cctctttggg tggcggacat ggattttgtt
       61 tcggccgaac ccatcctccg ggcccttcat caacgggtgg atcatgcttt ctttggttac
      121 accatcccat tgggggagct atgcggtacg atcctcaatc ggttgaaagc cctgtatcaa
      181 tgggaagtcc gtgaggatga ggtcttttat ctcccgggcc tggttacagg attgaatttt
      241 gccgttcagg ccttttccgt acagggagaa ggggttctag tgcaacctcc ggtctatcat
      301 cattttgtaa aagacccagt ccatcacggg cgcgtccttg tcgaccctcc cttggtagaa
      361 aacggggatc gttatgaggt ggatttcgaa agttttgaaa aagccattac cccgcaaacc
      421 aaaattttca ttctgtgcaa tccccacaac ccggtcggcc gcgttttcac cagggcggaa
      481 ttggaaaaaa tggccgaggt ctgcttgcgc caccgggtcg tgatctgttc ggacgagatt
      541 cattgtgatt tggtcttccc ggaatttcgc catattccca ttgccagtct cagcccggag
      601 gtggcccgat ggaccattac cctcatggcc ccgagcaaga cctacaatct ggccggtttg
      661 cggtgcggtt ttgccgtaat tcagaatccc gagttggcca aaggctggag gaaggcgact
      721 ttggggctca accctgggtt gaacatcatg ggacaagtgg cggccctggc cgggtaccgg
      781 gaagggaaag agtggttgga tcaggtcctt gcatatttac gcacaaaccg ggattttctc
      841 catcggacca tcccggagaa acttcccggt gtgaggatga ccaagatgga ggccacctat
      901 ttggcctggc tggactgtcg taagacaggg attccgggga atccttccca gttcttcctg
      961 aaagaggcca aagtagccct caatgacgga ggggaattcg ggagaggcgg ggaaggtttt
     1021 gtccgcctga atttcgcctg tccccaggag atgctgaatc aagctttgga gcagatgtcg
     1081 tgggccatgc agaagctccc ataggaaggt tcgataatcc gcccattcct gagaagacat
     1141 ctgttgcgga acaaggcctt gtttttccgg gaggctgaat gcatttccgg tcttctctat
     1201 ctctcgatga aacaaagaaa cagcggagag aggtgaaccg catttctgcc cttcctggct
     1261 gaagtaatgg actaggaagg tgcatcgacc gccgggtatc cggcggccag aaatccccca
     1321 gatgaattgt ggagggttta aaagagagcg tgtcggccga acttcgcccg acctgagagt
     1381 ttttaacccg ccgcttgtca ggattctccg agggctgaat ggggaagaaa cttcaggcct
     1441 cctgtggccg gaagtgggtt ggagcgggcc aggaggagaa tctcagatgg agggcccctt
     1501 ttcccggaga attcttttga actccccgag atagtcatcg acgggtaatt tccatttttc
     1561 gggttcgagg ggcatgatga tggaacgttt gtaccaggag gattggtctg ctttttcatt
     1621 caaatcggct agaaagggct gggggtaaag ggtcttagaa ttctgataga tgataagcac
     1681 attagggcgc aggagcatct caggtttcac cccgcaaacg acccccattt cctctgtatc
     1741 caaaagaacg aaggtcccaa aaggatagat cccgatgcat tggatgaatc tttccaccat
     1801 caatcggttg aattctacag gtccctcttc gtagagcttt cggatcgctt cgtggggctg
     1861 aactgctttt tggaaaggac ggtcggtggt gaccgcgtcg taaaaatcga tgatcccggc
     1921 gatctggccg ccgaggctga tggcctctcc cttgaggccg aagggatagc cgtttccgtt
     1981 gtagcgctcg tggtgctgga gggccagctg cttggatggc tcggggatgc ctttggattc
     2041 ctccatgatc tccaggctgt aaaccgggtg ttttctcact tccacccatt ctttttcact
     2101 gagggtatcc ggctttttca gaatacccgg atcgatcctc aatttgccta tatcgtgcaa
     2161 gagcgctcca attccgatct ccaccatgtc ttcccgggga aggtttagat gccgtgccat
     2221 ggataggctt aggatgcaga catcaaggga gtggacaaag atatattcgt cgtaaatcct
     2281 tatccgggcc aggctggcga gggcttcgtg gtttcgcagg acgctgtcca ccattgaatt
     2341 cacagcccgt ctgacccggt ctccctcgat gctctttccc atccgcacct cgttgattac
     2401 attccggacc acggtctgcg cttccgcctt gaccttccgg gcgccatgaa tttctttgat
     2461 gaagggaatc tcgtcatcaa aggggatgcg aaggggggat tccggggaga acagactctc
     2521 ccccacctgt ccgatcttct cctcccaaaa agccacagaa tcagattttt ccatcctttc
     2581 gaaaggaact tcttccttgg gttcatcgac cgccccaccc ttatatgggt gagatggtgc
     2641 ggcttcaagg gtaggaacaa gctctgtgac ctttttcgaa taagcctcga caggttgcga
     2701 tggaggagag ggagcctcag aaggcaatgc ttttacctct tccggtcttt tatcggttaa
     2761 attcgggcca ggatttgatc cttcatctcc cgaagggata gcgccccccc cttcaacctt
     2821 tatggaagga acgtcgagac cctgctgggg gtctatgtaa acctcctgaa ttccgtactt
     2881 ttttagcttt ccaatcttct tgggagagtc aatcttaagc tgattcctaa agaaaggatg
     2941 acttatccaa gaacgaccta ggttgacgat gaacattccc acttttagct cgtctacacc
     3001 gattttcttc aattttgttt tgtcagcgtt catggtcatt gaatttttta ttcctctgcc
     3061 tttttataag aaaatatatt tttttattga ttttaataag ttagcctgga tttctggctt
     3121 ttgcttttca attttctgaa ggcttcagcc cccgcgaagg gtgagtggca aaatagaagc
     3181 ccggaatgtc cctcaacggt cttcaaagcc gctctcttat aatatcgttt gaaattccca
     3241 agaccttgaa tgatttaagg tagttttgtg ggtcccttgg gtgtgaacct tggggcggca
     3301 ggggataaaa ttatatcaga gccaggcggt cggcaacagc caggccctcc cgggttgggc
     3361 gaagataacc ttcctggacg acgatcagcc cctcttcctc aagcttctgc aagatttttc
     3421 tcctctcctg gagaaaattt tctccgtgat tttttgaaaa atcattcagg ttgatccccc
     3481 ttgcagtccg gaatcccagg aagaaagcct ctgtctgaag ctgctccggg gtgaggattt
     3541 cgaagttgtc caagggcagg gttcccgcac ggagagtgtt catgtaggcc gtgattgaag
     3601 gggtattcca ccagcggacc gatccagaga aagaatggga ggatgggccc agacccaggt
     3661 aaggagtatg attccaatat ttttggttgt gacgggaggc attctgcaaa ccccgggaaa
     3721 aattggaaac ctcatagtga ataaatccta ccttctccag cgattccgag gtggccagga
     3781 agaattgacg gaggtcttcc tctttgggaa atgcaaaagt cccccggcag tgggctttcc
     3841 ccatgggggt gtcctcctca agggtgagtt gataacagga cacgtgctcg ggggaaaagg
     3901 tgatggcgcg tcgaagagta tccagccagg attccagggt ttgcccggga acaccgtaca
     3961 tgaagtccaa tccgagatga tgaaaaccgg cttcgcggga tgcttcgatg gcttggatgg
     4021 ccttttcggc gtcgtgtctc cgccccaaga agaacagaac atcgggggca aaggattgga
     4081 cgcccaggtt gagcctatga atccccaatt ctctaagggc ctgcagaaaa gaaaaatcca
     4141 ggtctccagg attggcctcc agagtgaatt ccgtcgtacg ggctaaaggg aaatatttgc
     4201 gggtttttcc aagaatctct tcgatttgac ggggactaag gacggaggga gtgcctcctc
     4261 cgatatagac cgtatcgaaa ggggatgaaa aagtctggct gaccatctcc atttcttttc
     4321 ccagggcttc gataaagccg gggatgagcg acaggtcggt ttgcgaatag aaactgcagt
     4381 agggacattt cgttacgcag aaggggatat gaatgtagag gccgggcggt ccttgatttc
     4441 ggatttcgaa ttgcggattt cggattgacc ctttcgggtt ggggaactgc tttgcctcag
     4501 tcatcattgc ttttcaggac cgccagaaac gcgctctgag ggatggtcac ggacccgatc
     4561 atcttcatcc gcctcttgcc ctctttctgc ttttccaaaa gctttcgctt ccgggtgatg
     4621 tccccgccat agcatttagc ggttacatct ttccgatagg gagaaatggt ggtccgggcg
     4681 atgatctcgc cgccgatagc cccctggatg gccacccgga acacctggcg gggaatctcc
     4741 tccttcagcc tttcgcaagc ctgaagggcc cgggaacggg catggccgcg gtggaggacc
     4801 tgggaaaggg cgtccacctt gtccccgttg accaggatgt caaggagaac caggtcgctt
     4861 tcccggtaat ccaggaggtc gtaatcgaag gacccgtatc cctgggtgat ggttttgaat
     4921 cgatcgtaga aatcgtagat cacctcggcc aggggcattt cataagtcaa ttccacccgc
     4981 ttgggaccca ggtagttcat cttggaattg acccccctgc gatccgtgca gagcttcatg
     5041 acccctccca agtatttatc gggtgcgatc atagtcgccc ggatgtaggg ttcctcagcc
     5101 ttgatgacgt tcgctcggtt cgggtagtgg gccgggttgt ccacgtcaac catgctccca
     5161 tccttgaggt tgaaccggta ccgaacgctg gggacggaaa gaatgatgga aagattatac
     5221 tcccgctcca gcctctcttg gacgatttcc aggtggagaa gtcccagaaa gccacaacgg
     5281 aacccctgcc ccagagcgac ggaggagtcc ttctggtaga taaaggccgc gtcgttgagc
     5341 ttgtattttt ccagggctac ggagaggtcc tcgtaatcgt ccgaagcaat ggggtagatg
     5401 gaggagaaaa caaccggttt tatatccttg aagccaggaa gcggccggcc gcaagatcgg
     5461 ctgtcgaggg tgatggtgtc gccgatgcga acgtcggaga tggttttgat gccggcgatg
     5521 atgtaaccca cttctcccgc agccagctgc tccatgcgtt cccggcagag ggaaaagcgg
     5581 cccacttcct ccacttcata gtcggcttgg ttggagagaa agcggatcgt gtcccctttc
     5641 ttcacggccc cttcaaagac cctgcagtgg acgatggtgc cccggaagga atcgtactga
     5701 gcatcgaaga tcagggccgc gaggggggat tcgggcgatc ctctgggagg ggggatcttt
     5761 tgaacgattg cctccagaat ctcttcgatc ccgattccgt ccttggctga gcactttagg
     5821 tgggtggcgg gatcgagtcc caattccgtg tcgatctgct ccaggacccg gtccacatca
     5881 gccgttggaa ggtcgatctt gttgatgacc gggatgattt ccaggttgtt ctccatggcc
     5941 aggaggaggt tggcgatggt ctgggcctgg actccctgag cggcatcgat gagcaaaagg
     6001 acgccctcgc aggacgcgag agcccgggag acttcatacg tgaagtccac atggccgggg
     6061 gtgtcgatca ggttgaggag gtaatccttg ccgtccttcg cccggtaggg aagggtcacc
     6121 gcctggcttt taatggtgat gcctcgctct ctttcgatgt ccatgttgtc caggatctgg
     6181 tcgcggaaat tccgctcctc caccagtccg gtatactgga taaatcgatc ggccagagtg
     6241 gatttcccgt ggtcgatgtg ggcgataatg ctgaaattgc ggatgttttc aggcatggat
     6301 tttatgcacc ccacccagga gagcgattct cttcggggca ctggatcgaa ttctcagggg
     6361 aatcaccaaa gaaactcgac aggatctttt ccctggaaga aaaacctagg tgggttttaa
     6421 taatatttta tcacaggccg gtgcacattt catcgttttt cccgcctgct ggcgataccg
     6481 atggaaattt atcattttga ccgtggctat gagagattaa caaaaaagag gggacctctg
     6541 aaaggattca tttcgagtcc tttttggagg gcggcaactc cttcttggag ggcgtcaact
     6601 cctggacctc cttttgaatg gatgctcttt ccctaggtga atcgttgtaa taggggaggg
     6661 ctctttggaa ctgagccagg gcggtcttcc cgtctcccaa gagtttatag tagtatccga
     6721 ggctccggta ggcggctccc aacatatttt tggccccgta ggccatagcg aggtgataat
     6781 agttttctcc aaagttgggg tccagtttat tggctgtcag aaaggcctgg agggcatcgt
     6841 ccaccctttt ctgctccagg taaacccttc ccagatagaa ataagctttg gcgtcggaag
     6901 gagaaagaga acgggcctgt tccgcgtatt tttgggcttc cgcgacgttt gctttgagaa
     6961 aataagctgc cgccagttcg cggtaaattt ccccgtcttg aggggccagg gaagccgcct
     7021 gggacaagtt atctatggac cggtccaagg ctcccatctt cctctgggcc aaacccaggc
     7081 cgaagaggca ttccgggtca tccggctgcc ttttccggca gttttcgaag taagtcaccg
     7141 cccgggcggt gtctttttct tcacacacca gtttggtctg gacgcgcttc aaatttcctt
     7201 ccgggggccg cctgttctcc gtcttgggta aattcatcat ctgaatttcc atgtttccca
     7261 tcctctgctc agcggccggg tgggtgagga ggtaggcggg agggtcggtg gatgcgggtc
     7321 cagaaatccg gcgtaatttc ttgagcatgg tgaccatggc ctgcggatca tatccggcgc
     7381 gctccatgta tttcagaccc aggtagtcgg cttcctcttc atactcccgg ctgtatttca
     7441 gcatggcggt ggctgaggcg gcctgtgccg taacgccgat ggcggtcgcc gcctggggcc
     7501 ccaggaagat tcctgccagc agggctccca gggtggctag gcccagtttc atgccctgct
     7561 ccgattgccg ggcaatgtgt cgggccagga cgtgtccgat ttcgtgggcc atcacgcccg
     7621 ccagctcgtc ttctttttcc accatgagga tcaagccgct attgaaaaag actttcccgc
     7681 cgggaacggc aaaggcgttg atttccgggg tattcaggac gtagaactca tattcaaagg
     7741 gctgagcctc catggtggcc aaaactcttt tcccgatccg ggcgatatac tcccgggggg
     7801 cggggtcctg caccatgggc cagatcttgc gcacctcctg gaggaccttc tcacccaatt
     7861 cccgttcttc ctggatggtc attgctgaga cggggggagg ggtgtagagg acagaaaaaa
     7921 ggaaaaccac cgccagaaag acggataacc gtcgggaaaa tgaggacttc agtcgagtca
     7981 tagcctattt tagcgcgatt cgggggcaaa gtccaatcca accccaagga tcccgcctgg
     8041 aaattgatgg tttgttgttg gcaaccgcga gccccgcctt cacggggaaa cattttgtta
     8101 gccgtattag taactggagg gttttataaa gaaagaattc gccttattcc aggatcaagc
     8161 ggtccacggc gaattgatcg accttcagcg agtccagccg aaggcgataa tccggggcca
     8221 ggagaatttt ccctcctgcg attttcaatc cttcagccag gattgacccc ttctgcttca
     8281 ggaatccgtt catctctccg gcgtggattg acatgcgtcc ggccaccagg gcttcctgat
     8341 aacagctgac ctcgccggcc gcgccgttcc ccagaatgat cccgaaaccc attcgacggg
     8401 aaatctcaat cgcgtgagcc aaggcttccg cgctgcccat tttcatgatc ttgaatttga
     8461 cgaagcgagc acagccaagt ttctttgctt tttccacgga atccatcccg taaatcgatt
     8521 cgtccagacc cagaggaagg ggagagacct tggagagttc caccatggaa tcccaatcat
     8581 tctctttaaa aggctgctcc agaaattcga tcccatgagg atcaatgctc tggacaaacc
     8641 tcttggcatg gagaagggtg tacccctgat tggcatccgc ccgcagatgc gcccttcctt
     8701 ggatcatctc ttgcgccagt tttacccttt caatgtccct atccacatcg aaaccaacct
     8761 tgatctttat ggtgtcataa ccgcgggaaa tgaaatcttc gatttccatg cgaatccctt
     8821 ccgggtccgt cgcctggaga atccccagca tgggcacaga tagaagctgg gaaggagggg
     8881 caagaaggga cttcgggttc agcatttcca gggcggtcaa aaaaggggtc accgagcagg
     8941 gcatttcctt cgcatgcctg gaaatcgaat cccgggcttt ttccaccggg aggccgagaa
     9001 tctttgggct ttgttctttg gcaaactgcc agacctgatc ggcggtctcc caaaaatagc
     9061 cctgaatatc tgccatggcc tctcccagtc cctc
//
LOCUS       DASPRR010000630         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_966588, whole genome shotgun sequence.
ACCESSION   DASPRR010000630 DASPRR010000000
VERSION     DASPRR010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966588"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..892)
                     /locus_tag="VEL68_23100"
     CDS             complement(107..892)
                     /locus_tag="VEL68_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXZ38936.1"
                     /translation="MPNSSAPVRIEKKGGITTVILSRSEVRNAVDSATAKALADAFRS
                     FEADSDAKVAVFFGDHGTFCAGADLKAITHGDRQADIKKVFEGDGPMGPTRMVLSKPV
                     IAAVAGYAVAGGLELALWCDLRIMEEDAILGVFCRRWGVPLIDGGTVRLPRLIGLGQA
                     LDLIITGRPVTAQEALAMGLTNRVVPKGRSREAAEKLALEISSFPQACMNADRMSAYT
                     QWDLPLLEAMRNEFERGIPMVEIEGRSGAARFAGGAGRGGKFE"
     gene            complement(1151..1855)
                     /locus_tag="VEL68_23105"
     CDS             complement(1151..1855)
                     /locus_tag="VEL68_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38937.1"
                     /translation="MLEVKKINLGYGQMQVLKEVSLHVGDREIVSLIGANSAGKSSML
                     NAISGLVPISGGEIRFEGKRIDSLPSHEIIEYGIIQVPEGRRIFPFMTVKENLEMGSY
                     NRRAREKNRDSLQRVYEHFPRLAERKSQLGESMSGGEQQMLAIGRGLMAGPKILMLDE
                     PSLGLAPIVVEMIFNVLQEVNRQGVTILLVEQNVKESLDISTRGYVLENGRVSLEGAA
                     GDLLKNSYLKKAYLGL"
     gene            complement(1883..2842)
                     /locus_tag="VEL68_23110"
     CDS             complement(1883..2842)
                     /locus_tag="VEL68_23110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38938.1"
                     /translation="MRFLKGNTFLGFLILLALLVAVVQNAFVLHLLIMVFTSAALGLA
                     WNFIGGYGGQLSFGHAAFYGIGAYTSTLLFVHLGLTPWLGMIAGGILAVVASLILGIP
                     CFRLRGTYFTLATIAFAEVLRILVVYFKEFTGGSIGIVLKFNAGFLNMMFKNREPFAY
                     VALGFMILTYFLSVWIENSRLGYYLTALKENEDGARALGINTHRCKLIALMISAFLTA
                     LIGSFFAQYLTFIEPESEFSLGLSIGLTLSVMIGGIGTAMGPVIGAFIITPLQELLRI
                     YISGEHQGLQNIIYGIVLVVVVICIPQGLYKWIVGTVLKKRAA"
     gene            complement(2856..3725)
                     /locus_tag="VEL68_23115"
     CDS             complement(2856..3725)
                     /locus_tag="VEL68_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38939.1"
                     /translation="MLIIVGQSLISGILMGGIYALVSIGLTLIFGVMNVVNFAHGEFL
                     MLAMYLTFWVFHYFQIDPYVSLLIVTPALFLIGVVTQKYLIQHLIQAEHYTQIFATVG
                     LSTIMANAALFFWKADYRMVKTSYVSSTLTFSDLVVSYPRLISFLVAIGITVLLFLFL
                     KYTYLGRAIRATAQDIIGARLMGVNINRVYIFTFGLGSACVGIAGALLMPIYYAFPTV
                     GLHFVLTAFVVVVLGGMGNITGALFGGLVIGVVEAFSGFFISPELKEAVYFVIFVLVL
                     LVKPSGLFGLTKG"
     gene            complement(4043..>4771)
                     /locus_tag="VEL68_23120"
     CDS             complement(4043..>4771)
                     /locus_tag="VEL68_23120"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38940.1"
                     /translation="DQVKLGVVYEDSLFGTTQAKYGFEAAKKAGIIVVADIPYNAKST
                     DLTPVILRLKSAKPDFLLYPGYFTDQVLFIRQSKELGFDYKIALTTGGSGLKDFGKAL
                     GDDVEGVGLIGFTSMDVNPKFAKGVKEFAALHIKLLGREADGPYPLMNYMGTKVLFQA
                     VQKGGSIEPEAIRKGALSIDIPPEGTETGGGVKFDPKTGQNQRAVTPVAQWQNQQMVT
                     VWPEKAAIAKMQFPLPTWAERKKK"
BASE COUNT         1129 a         1366 c         1307 g          969 t
ORIGIN      
        1 cttcagaagg cataaacctc tcgaatcctt aagatttgcc tgattataca gaattggcgc
       61 ggctttgtcg agcttgtagg aatgaggcat ggttcgcccc tacagattat tcgaatttcc
      121 caccccgccc cgctcccccg gcaaaacggg ctgcccccga acgtccttcg atttccacca
      181 tcgggattcc cctctcgaat tcgttgcgca tggcctccag caaaggtagg tcccactggg
      241 tgtaggccga cattcggtcg gcgttcatgc aggcctgggg gaaggaactg atttccaggg
      301 ccagtttttc agccgcttcc cgggatctac ccttggggac gacccggttg gtcagaccca
      361 ttgccagagc ttcctgggcg gtgaccggcc ggccggtgat gatcaggtcc aaggcctgcc
      421 ccagcccgat cagacggggc aggcgtacgg ttcccccatc gattaaggga acaccccagc
      481 ggcgacagaa cacgccgaga atggcgtctt cctccatgat tcttaggtcg caccagaggg
      541 caagctccaa accaccggcg acggcatatc cggccaccgc ggcaatcact ggcttggaga
      601 ggaccatgcg agtcggcccc atgggtccgt ccccttcaaa aactttcttg atgtccgcct
      661 gacgatcccc gtgggtgatc gccttcaggt ctgcgccggc gcaaaaggtt ccgtggtctc
      721 cgaaaaagac cgccactttg gcatccgagt cagcttcgaa agatcggaag gcatccgcca
      781 acgccttggc cgtggccgaa tcgactgcgt tcctcacctc cgatcggctc agaatcaccg
      841 tagtgatgcc cccctttttt tcgattcgaa cgggtgctga gctatttggc ataaatcccc
      901 ctttcaggac aaacgcctgc gctgccagat tcctcccgaa atttctcccg cccaaggact
      961 ggcggcgagt ttcaagaaga gttgacaggc aaaggtcttc gttcccgaga attgcagttt
     1021 aacccattta gaccaaaaga tgagcctaaa agttttgcaa attagttgaa gagtcggggt
     1081 aagattgata ccccacccca aaagagtatt tagcaaagag taaccagtaa ccaataacca
     1141 ataacagtct ttacaacccc aggtaggctt tttttaggta ggaatttttt agcaggtctc
     1201 cggcggcccc ctccagggaa acccgtccgt tttccagcac gtatccccga gtggaaatat
     1261 ccaggctttc cttcacgttt tgctccacca gcaggatggt aaccccctgg cggttgactt
     1321 cctgcaggac gttgaagatc atctccacga cgatcggcgc caaccccagg gagggttcgt
     1381 cgagcatgag gatcttcggc ccggccatga gcccccgccc gatggccagc atctgctgtt
     1441 cgccgccgct catggactcg ccgagctggc tctttctctc agccagccgg gggaagtgct
     1501 catagacccg ctgcagggaa tcccggttct tctcccgggc cctgcggttg taactgccca
     1561 tctccaggtt ttccttcacc gtcatgaagg gaaaaatccg ccgtccttcg ggaacctgaa
     1621 tgatcccgta ctcgatgatt tcgtgggagg ggagggaatc gatccgtttc ccttcgaagc
     1681 ggatttctcc cccggaaatg ggaaccaggc cggagatggc attcagcatg gaactctttc
     1741 ccgccgaatt ggccccgatg aggctgacga tttcccggtc cccgacatgc agggacacct
     1801 ccttcaaaac ctgcatctga ccgtagccga ggttgatttt cttgacttcc aacacggatt
     1861 tcccctttcc cctaccgtcc gatcaggcgg ctctcttctt gaggacggtt cccacaatcc
     1921 atttatatag gccctgggga atacagatga ccaccactac aaggacgatc ccgtaaatga
     1981 tgttctggag gccctggtgt tccccgctga tatagatccg gagcaattcc tgtagggggg
     2041 tgatgatgaa agcgccgatc accggcccca tggctgtgcc gatgcctccg atcatgaccg
     2101 aaagagtcag gccgatggac agaccgaggg agaattccga ctccggttca atgaaggtga
     2161 ggtactgggc gaaaaaggag ccgatcaggg cggtcagaaa agcgctgatc ataagggcga
     2221 tgagtttgca gcggtgggtg ttgattccca gggcccgagc tccgtcctca ttttccttca
     2281 gggcggtgag gtagtaaccc aagcgggaat tttcgatcca gacagagaga aaataagtca
     2341 gaatcataaa gcccagggca acataggcaa agggctcccg gtttttgaac atcatgttca
     2401 gaaaacccgc gttgaacttc aggacgatcc cgatgctccc acccgtaaat tccttgaaat
     2461 agaccacgag gatccgcaga acttcggcaa aggcgatggt ggccagggta aaataggtcc
     2521 cgcggagccg gaagcagggg attcccagga tcagcgaggc aaccaccgcc aaaatccccc
     2581 cggcgatcat gcccagccag ggcgtgagcc ccagatggac gaagagcaga gtggaggtgt
     2641 aagccccgat tccgtaaaag gcggcatgtc cgaaagaaag ctgccccccg tacccgccga
     2701 tgaaattcca ggccaggccc agcgcagccg aggtaaagac catgatcaac aggtggagga
     2761 cgaaggcatt ctgcaccacg gccaccagca gggccagaag gatcaaaaaa ccgaggaagg
     2821 tatttccctt tagaaacctc atcgatgaat atcctctatc ctttagtcaa cccgaacaaa
     2881 cccgacggtt tgaccaggag aaccagcacg aaaatgacga aataaaccgc ctccttcaat
     2941 tccggggaga tgaagaaacc gctgaaggct tccacgaccc cgatgaccaa ccccccgaag
     3001 agagccccgg tgatgttccc catgcccccc aggacgacga ccacgaaggc ggtgaggacg
     3061 aagtgcaggc ccacggtggg gaaggcgtag tagatgggca tcaacaaggc ccccgcgatc
     3121 ccaacgcagg ccgagcccaa cccgaaggtg aagatgtaca cccggttgat gttcaccccc
     3181 atgagcctgg cgccgataat atcctgggcg gtggcgcgaa tcgcccggcc gagataggta
     3241 tatttcaaga acaaaaagag gagcaccgtg atgccgatgg ccaccagaaa ggaaatcagt
     3301 cggggatagc tgaccaccag gtcgctgaag gtcaaggtgg aggacacata agaagtcttg
     3361 accatccggt aatcggcctt ccagaagaag agggcggcat tggccatgat ggtggaaaga
     3421 cccacggtgg caaagatctg ggtgtaatgc tctgcctgga tgaggtgctg aatcaagtac
     3481 ttctgggtga cgaccccgat aagaaagagg gcgggagtta cgatcaggag agaaacatac
     3541 gggtcgatct ggaaataatg gaagacccag aaagtaaggt acatggccag catgaggaat
     3601 tcgccgtggg cgaagttgac cacgttcatg accccgaaga tcagggtgag gccgatgctc
     3661 accagagcgt aaatcccccc catgaggatt ccgctgatga gggactgtcc gacgatgata
     3721 agcatagggt tgttctctta aagatacttt tctcccgatg gagacaacaa attgggtgct
     3781 ttaccaatga gtccacaata gcctaaacag tattgattgg aggacttccc cctatgaaaa
     3841 cctgaaactg gactccggct ttcgccggag tgacgaacat gaaagggtac ggttctttgt
     3901 tgtcattccg gcggaagccg gaatccaggg aagcaaaagg tgaaagccat atccgcctat
     3961 tatggcctca ttagtaaaca ggggcacgcg gcggcgtgcc cccacaaccc gagactcgaa
     4021 acccgcgacc cgaaacggtt tctcactttt tcttccgttc cgcccaggtg ggcagcggga
     4081 actgcatctt ggcgatggcc gctttttcgg gccacaccgt gaccatttgc tggttctgcc
     4141 attgagcgac gggcgtaacg gccctctggt tctgtccggt cttgggatca aatttcactc
     4201 ctcccccggt ctccgttccc tcgggaggaa tgtcgatgga cagggcccct ttgcggatgg
     4261 cctcgggttc gatggaccct cccttctgga cggcctggaa gaggaccttg gtccccatgt
     4321 aattcatcaa cgggtagggg ccgtcggcct ccctcccgag gagcttgata tgcagggcgg
     4381 caaattcctt aacccccttg gcgaatttcg ggttgacatc cattgaggtg aacccgatca
     4441 gcccgactcc ttccacatca tcccccaggg ccttcccgaa atccttcagc cccgaacctc
     4501 cggtggtgag agcgatctta tagtcaaatc ccagttcttt cgattgacgg ataaacagga
     4561 cctgatcggt gaaatagccg gggtagagca gaaaatccgg tttggccgat ttcagccgca
     4621 gaatgaccgg agtcaaatcg gtggacttgg cgttgtaggg aatatcggcg accacgatga
     4681 tcccggcctt tttggccgcc tcaaacccgt actttgcctg ggtggttccg aaaagagagt
     4741 cttcgtatac cacgcccaat ttgacctgat c
//
LOCUS       DASPRR010000631         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_156720, whole genome shotgun sequence.
ACCESSION   DASPRR010000631 DASPRR010000000
VERSION     DASPRR010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156720"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VEL68_23125"
     CDS             <1..564
                     /locus_tag="VEL68_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXZ38941.1"
                     /translation="LAMVLDQVHDLIILDIALPKIDGIQILKKLRERKITTPVLLLTV
                     RATIEDKVLGLDFGADDYLTKPFVFQELLARIRALLRRKSEAGLPLLRIEDLVLDPSR
                     HLVTRGGERIDLTSKEFALLEYLMRNAGRVLTRAMISEHVWNYDFDTETNIIDVYVNY
                     LRRKIDSGREKRLIHTIRGSGYVLKSE"
     gene            568..1956
                     /locus_tag="VEL68_23130"
     CDS             568..1956
                     /locus_tag="VEL68_23130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ38942.1"
                     /translation="MPLKSIRARLTFWYTSLLTLSFLILGSGGYWLLTYTLSQESDAA
                     LRGVARTLAERVGGGTQTFIPSEIDEVFRQFFGFSPLEHYFQRILPESPSSRGGKLPL
                     SEQALKNASRGIPTFETLEGLGPYPVRVLTWPVVRAGRVSGVIQVGMSRENLDKTLRR
                     FLWVMAGIFPVALLLAGGGGWTLARKALKPVDQMTETARRISAEHLDRQVQGSGAGDE
                     LDRLAQTLNEMLGRLNIAFQEVRQFSADASHELQTPLTILKGEIEVGLRSPRRPEEYQ
                     AILRSALEETDRIARLVEGLLLLSRSDAGVLRMDRRPLSLKNLIEEVMQEVQPIAGSR
                     SVDLRIGTMEPAQVLGDPDHLRRLLLNLVDNGIKYTQPGGSVEISLRIDGKWASLQVS
                     DTGMGIAEEDQEKVFRRFYRTAAARDRGGSGAGLGLAIARSIVEAHGGRIQVESAVGK
                     GSLFTVSLPLAS"
     gene            2277..3764
                     /locus_tag="VEL68_23135"
     CDS             2277..3764
                     /locus_tag="VEL68_23135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXZ38943.1"
                     /translation="MFSKVRMKWGNKKFRWTAMTAVAISFLFAGLLLASPLQITKPAA
                     AGDSRPTAQETVSGPVFPGSFSDLVQKQGSTVVNIKVTKIEKTGFPQEFSSPEGPYGD
                     LFRHFFRGMPQPPEAFRMQGAGSGVIISRDGYIITNYHVVEGAKDITVTLADRQEYKA
                     RVVGRDPKTDLAVLKIDPKEALNPAALGNSEQLKVGDWVLAIGNPFGLSNTVTAGIVS
                     AKGRIIGAGPYDDFIQTDAPINPGNSGGPLFNLKGEVVGINTAIVPNGQGIGFAIPVN
                     VAKPIIPQLISKGEVTRGYIGVSIQAITPELAKAMNLKDAKGALVSDVVPGSPADKGG
                     MKSGDVVTAYNGKNIDGPRDLSSWVAQTAVGQEATVAILREGSEKQLTLKIEKFGAEE
                     VKGEKSASSDQGKWGLALEDLNPQMKGLFKPKSSQGVLVANVQPGSPADLAGVRPGDI
                     LLSVNRQPVNSTKQAVEMISKVKDSDSLLLLLRRSEGTFFAALSK"
BASE COUNT          904 a         1075 c         1144 g          851 t
ORIGIN      
        1 ctcgccatgg tcctcgacca ggttcacgat ctaatcatcc tcgacatcgc cctccccaaa
       61 atcgatggaa ttcagatcct taaaaagctg cgggaaagga aaataactac cccggtttta
      121 ctcctcacag tcagagcgac cattgaggat aaggtcctgg gcctggattt cggagcggat
      181 gattatctta ccaaaccttt cgtttttcag gaacttctgg ccaggatccg ggccttgctc
      241 cggcggaagt cggaggccgg actccctctt cttcggattg aagacctggt cttagacccg
      301 tcccgccacc tggtcacgcg aggaggagag agaattgacc tgaccagcaa ggaattcgcc
      361 ctcctggaat acctcatgcg caatgccggc agggtgctga cccgggccat gatcagcgag
      421 cacgtctgga actatgactt tgataccgag accaatatta tagatgttta tgtgaactat
      481 cttcgccgca agattgattc cggacgggag aagaggttga tccatacgat ccgagggagc
      541 ggatatgtgc tcaaatcgga gtgacccgtg cctttaaaaa gcatccgggc tcggctgact
      601 ttctggtaca cgagcctcct cactttgagc ttcctgatcc tgggaagtgg gggatattgg
      661 cttttgacct acaccttgtc tcaggagtcg gatgctgcgc tccgtggagt cgctcgaacc
      721 ctggccgaac gagttggcgg cggcacccaa actttcatcc cttctgaaat cgacgaggtt
      781 ttccggcagt tctttgggtt ctcccctctg gagcactact tccaacgcat tctccctgaa
      841 tccccctctt cccgcggggg aaaactgccc ctgagtgaac aggccttgaa gaatgcctcc
      901 cggggcattc ccacattcga gacgttggaa ggcctcggcc cttatccggt ccgggtgctg
      961 acctggccag tggtcagagc cggtcgagtt tccggcgtca ttcaagtcgg aatgtccagg
     1021 gaaaatctgg ataaaaccct ccgccgattt ctttgggtta tggccggcat cttcccggtc
     1081 gccttgctcc tcgccggggg agggggatgg accttagccc gcaaggctct gaaacccgtg
     1141 gaccagatga cggaaactgc acggaggatc agcgcggagc acctggacag gcaggttcaa
     1201 ggatcgggag caggggatga actggaccgc ctggcccaga cgttaaatga gatgctgggc
     1261 cgtttgaaca tcgccttcca agaggtccgt cagttcagcg ccgatgcctc ccatgagctt
     1321 cagacccctc taaccattct gaaaggcgaa atcgaagtcg ggcttcgatc tccccgaagg
     1381 ccggaggagt atcaagccat cctgcgaagt gccctggagg agaccgatcg gatcgcccgg
     1441 ctggttgagg ggctgcttct cctttcccgg tccgatgcag gggtcctccg catggaccgg
     1501 cgtccattga gcttgaagaa tctgatagaa gaggtcatgc aggaagtcca gccgatagcc
     1561 ggatcgcgtt cggtcgacct ccggatcgga accatggagc ccgctcaagt tctgggggat
     1621 cctgatcacc tcagaagact cctcctaaac ctggtcgaca atgggatcaa atacacgcag
     1681 cccggaggaa gcgtagaaat ttctctccga atagatggga aatgggccag cctacaggtc
     1741 tcggacacgg ggatgggcat cgccgaggaa gatcaggaaa aggtcttccg gcgcttttac
     1801 cggactgccg cggcacggga ccggggcgga agcggggcgg gcctaggcct ggccatcgct
     1861 cggtccattg tcgaggccca cggggggcga attcaggtgg aaagcgccgt gggcaaaggc
     1921 agccttttta ccgtctctct cccccttgct tcttaacccc ccacccaaac cctcccccct
     1981 cgaagactgc gtcgtaatcc cccggtcgcc cttcgacagg ctcagggcga acggccaagt
     2041 tgctgaattt caatgcactc ataatccgtt cgtggtgagc ttgtcgaacc atgaacggat
     2101 tgcgacacag tctttcgaag ggagggaagg gagggggtga tcccttgccc ttcattagaa
     2161 aattctaatc ctggtttcat cttcctttaa tgttgctccc ttatcttttg aatgaagcag
     2221 aagatgggat gcgcctgaaa ggcgctccca attccaacgg taagggaggt acgaacatgt
     2281 tctcgaaagt aagaatgaaa tggggaaaca agaaattccg gtggacggcg atgaccgccg
     2341 tggcgatttc tttccttttc gccggcctcc ttctggcctc accgctccag atcaccaaac
     2401 ccgcagcagc gggagactca cggccgacgg cccaggaaac ggtgtctggg ccagtttttc
     2461 cgggctcctt ttcggacctg gtccaaaaac agggctccac ggtcgtgaac atcaaggtca
     2521 cgaagatcga gaagaccggg ttccctcagg aattttcttc tccggagggg ccttatggtg
     2581 acttattcag gcatttcttc cgggggatgc cgcagccccc ggaggctttt cgcatgcaag
     2641 gggccggttc aggagttatc atcagccggg acggttacat catcaccaat tatcacgtgg
     2701 tggaaggggc caaggacatc acggttactc tagctgaccg gcaggagtac aaagcccggg
     2761 tcgtgggaag agacccgaag acagacctgg ccgtcttgaa gatcgacccc aaagaggcgc
     2821 tcaacccagc cgccctgggg aattcggaac agttgaaagt cggggattgg gttctagcca
     2881 tcgggaatcc cttcggcctg agcaacacgg taacggcggg gatcgtaagc gccaaaggcc
     2941 ggatcattgg ggccggacct tacgatgact ttattcagac ggacgccccc atcaatccgg
     3001 gaaactccgg gggtcccctc ttcaacctga aaggagaggt tgtggggatc aatacggcca
     3061 tcgttcccaa cgggcaagga atcggatttg ccatccccgt gaatgtggct aagccgatta
     3121 tcccccagct gatctctaag ggggaagtga ctcgggggta catcggggtg agcattcaag
     3181 ccatcactcc agagttggcc aaggcgatga atttgaaaga tgccaaagga gctttggtga
     3241 gcgatgtggt tccgggaagc ccggcggata aaggggggat gaagtcgggc gatgtagtaa
     3301 ccgcttataa tgggaagaat attgacggac ctcgggattt gtcctcttgg gtcgcccaaa
     3361 ctgctgtggg acaggaagct acggtcgcca ttctccgtga ggggagtgag aagcaattga
     3421 ccctcaagat tgaaaagttc ggcgctgaag aagtcaaagg ggaaaaatct gcctcctccg
     3481 accaggggaa gtggggcctc gcgttggaag acctgaatcc tcaaatgaag gggttgttta
     3541 agcccaaaag tagccagggt gttttagtgg ccaatgttca accggggagc ccggcggacc
     3601 tggcgggtgt acgtccggga gacattcttt tgagcgtgaa tcgtcagccg gtaaattcaa
     3661 cgaaacaggc tgtcgaaatg atttccaaag taaaagacag cgattcgctc ttgctcctcc
     3721 tgaggcgcag tgaggggact ttcttcgccg ctttgtcgaa ataaccaaaa gagagggata
     3781 gggaaaacct ggggggctga agatcgattc agccctcctt ccatttttca caagggaatt
     3841 ctatacggca acttcctgaa accattgcct ctccttgtat ctccaaaata tccctgctaa
     3901 gatgagctta ggaatatctc tgttaccttt ccccctactc ggcggggaga agagccgttc
     3961 ttattaaacc acaa
//
LOCUS       DASPRR010000632        10419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1124408, whole genome shotgun sequence.
ACCESSION   DASPRR010000632 DASPRR010000000
VERSION     DASPRR010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10419)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10419
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124408"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1478)
                     /gene="gltA"
                     /locus_tag="VEL68_23140"
     CDS             complement(60..1478)
                     /gene="gltA"
                     /locus_tag="VEL68_23140"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HXZ38944.1"
                     /translation="MTEPTEKKKKTQIPRTPMPEQEPKVRAKNFDEVPFGYNPELAKL
                     EASRCLQCKKPACVAGCPVSIDIPAFISLILQEDFRGAARKLKEMNSLPAVCGRVCPQ
                     EDQCEKVCILGKKGEPVAIGRLERFAADFEQKQGEFILPPMAPPTGKKVAAVGSGPAG
                     LTLAGDLIKKGHKVTVFEALHKAGGVLVYGIPEFRLPKDIVQSEVDYLEKLGVEIRTD
                     MVIGQIFTVDELMANGYDAVFLGTGAGLPTFMNIPGENLNGVYSANEYLTRSNLMKAY
                     LFPKYDTPIALGKKVAVLGGGNVAMDSARTALRLGADEVYIVYRRSKKELPARIEEVH
                     HAEEEGIKFHFLTLPVEILGKEDGWVCGMKCQKMELGEPDESGRRRPIPIPGSEFVLE
                     VDMVVVAIGTGANPLIPSTTLDLKTNKWGYIIADPETGATSKKGVYAGGDIVTGSATV
                     ILAMGAGRKAADAIHQYFDQLG"
     gene            complement(1478..2329)
                     /locus_tag="VEL68_23145"
     CDS             complement(1478..2329)
                     /locus_tag="VEL68_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HXZ38945.1"
                     /translation="MFPIVDAKLLAASVKQLRVAAPEIAKKRKPGQFVVLRTHEEGER
                     IPLTIADGDPAAGTITLIFQEVGKSTMHLGKLKAGDSILDLIGPLGRPTHIENFGTVV
                     CIGGGIGIAPVYPIAKGMRDAGNRVISIIGARNKDLLILEEEMAKVSHVLKVSTDDGS
                     YGYHGFVSDVLQDVIDEGNPLHLVVAIGPVPMMRVVCNVTRSRNIKTVVSLNPIMVDA
                     TGMCGSCRVSVGGKTRFVCVDGPEFDGHEVNFAELVKRQRAYLDQEQQASKIFLHPGG
                     ACIGGKS"
     gene            complement(2523..3104)
                     /locus_tag="VEL68_23150"
     CDS             complement(2523..3104)
                     /locus_tag="VEL68_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38946.1"
                     /translation="MRKTYFVIFIGMLAAWMALGACATAPKGASGGAVLTPKVELERV
                     EVASYFPYDPKVRVPLILAFIFKLENPNDFKVGLESIKFTVGFEAQGKPGEFFNLATP
                     MVYESQYIPPKTANQLRVTTVIDSQVVPGNLAVTSGARLAALGLKAPDLIQYWYEKIG
                     DFPFGIKVSEGSASFATDRGDVIVGFEGMFPKK"
     gene            complement(3221..4015)
                     /locus_tag="VEL68_23155"
     CDS             complement(3221..4015)
                     /locus_tag="VEL68_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38947.1"
                     /translation="MLKLNNIEVMYQEVILVLKGVSLEVPEGGIVALLGANGAGKSTT
                     LKAISGLLKHEDGEVTDGSIEFMGERIDRLGAEEVSKRGIIQVIEGRRVFEHLSVEEN
                     LRVGAHLRKDSSTVKDGLETVYQFFPRLKQLHAVTAGFVSGGEQQMLVMGRAMMARPQ
                     LILLDEPSMGLAPMLIKEIFDITMRLNREERVPILLVEQNVKLALTVAPYAYVLENGR
                     LVMDGTSERLIQNPDIRDFYLGLTDLGVRKSFRQVKHYKRRKRWLT"
     gene            complement(4121..5371)
                     /locus_tag="VEL68_23160"
     CDS             complement(4121..5371)
                     /locus_tag="VEL68_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38948.1"
                     /translation="MNEKRYWFLFSLILLLLSALPASAQQMVKLGALQDLTGATSDVG
                     KDEALGVKEAVQYYNDQGGINGKKIRLFQYDYGYRVPEAVTTYKRFRDYDKVVMVLGW
                     GTGDTEALSPTINADKMPYVSSSFSGHLCDPKKTPYNFVYGTDYSTAARGAITYWFEE
                     VWKKDPKWKAEREKGTKPRFVCFYMWASPYGSAPIKAIKDQAKILGFEVGPDQDVSLT
                     ALDTKSQVLAAKNFGPHLVWHGNTTMSVATALKDAMALGLGADHIINIWGFDENLVKL
                     AGPAAEGAIGLPACAFLGEEFPAKGSRVIPYAKKVSPNVSPETRLIRTVQGWCKVTLA
                     VEAMKIADKAGKLNGPGIREAFENNRISLKEFGGQVMNYTPTDHRATSVVRVARIKDG
                     KPMGVGEIDMKAKFPGEWPSWLGW"
     gene            complement(5541..6608)
                     /locus_tag="VEL68_23165"
     CDS             complement(5541..6608)
                     /locus_tag="VEL68_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38949.1"
                     /translation="MSFLPCGVYHEKYSQQHSWWQTAFVRGKMIFLFVFLLGILPLFA
                     GNYWLAVASLVGYTILSAMGVQLLIGYCGQVTLGHSAFIAVGAYTSAILMLQYQTPYL
                     LSLVAAGLVAGVWSVLFGLPSARVKGFYLIMTTMAAQFITVDFVITQYVSQIGGRGVA
                     LSIPPGTITLGPWAIDNDLKVYYLMIALVALCTLGLSNLMRTKIGRAWIAIRDNDIAA
                     ETMGVNIVAYKLMAFFVAGVLGGIAGAFWVSSLSALSPEHFTWSWSLWIVGVILIGGV
                     GSTHGTIFGALFMTLILEGLKFAVIPLSEDFPQLQEKFLFIKESTFGLAICLFLLFEP
                     KGLAYRWWQIKNYFNLWPFSY"
     gene            complement(6672..6875)
                     /locus_tag="VEL68_23170"
                     /pseudo
     CDS             complement(6672..6875)
                     /locus_tag="VEL68_23170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(7105..7989)
                     /locus_tag="VEL68_23175"
     CDS             complement(7105..7989)
                     /locus_tag="VEL68_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38950.1"
                     /translation="MEVFFQLIITGVMVGSIYALVALGWTLIYKCSGVLNLAMGELTL
                     IGAYVCLSFYNWGVPFLLALLGTLVIGFLLGLLTERLFLQRLIGEPLLTVIMVTVGLS
                     FFFKGIVAFLWGTDTQVFKPAVFPINPIKLGNIIIGQVYLWSFIAAIILLVIFVAFFK
                     YTRWGLSMQATADDEMAALSLGVSAKFVYALAWAIAFMASGVGGTLLGNINGLNISVG
                     YLGLLVLPAVVFGGLNSVPGAIFGGIIIGVLQNLADGYLSRFTPGGVKEIAPFAIMVI
                     VLLFKPYGLWGWVRIERV"
     gene            complement(7989..9938)
                     /locus_tag="VEL68_23180"
     CDS             complement(7989..9938)
                     /locus_tag="VEL68_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXZ38951.1"
                     /translation="MPRADELKYLDREIGPELTLPKLFQETCRKYWDRKVALREKEFG
                     LWRPITWKDYYEKVKWIALGLHSLGLQRGDKVAMIGDNRPEGLMVEMAALCAGGIGVW
                     LFQDSLIDEVAYIIDHSDSRFVMGEGQEEVDKALSIKGKCPKMEKVVWDDPKGLRGYE
                     DPLLISLKNVVQRGKELDQKEPALFDVLVRTGKGSDVALLFYTSGTTALPKGALLSHH
                     NMLTMGQNLMRVDPCYESDDFVSFLPFAWIGEQMMSLSCGLLAGFTINFPEEPETAQE
                     NIREIGPQVMFSPPRIYEQMVRTVQVKYLDATWIKRKMYEWSMAAGYRIADLKFAKKP
                     VPGWLRVLGLLAHFTVHKKLRDHLGVSRIRHTYTGGAAMGPDHFRFFHALGVNLKQIY
                     GQTEIAGISALHRDGDIKFDTVGKPIPGTEIKVTPEGEIISKSPSVFLGYYRMPAETA
                     KTLRGGWLYSGDTGFIDRDAHLVVFDRTKDVMVLPDGTKFSPQYLETRLKFSPYIKDV
                     WVIGDGRPFVAAVVCIDYSVVGKWAEDRNIPYTSYPELSQKAQVYDLVLKEIVKMNNG
                     LPKGARVRRFVNLFKEFDADDDELTRTRKIRRAFVEQRYREIMEAVYARQDVIHMNTT
                     ITYEDGTEVKIKANMRVADVSEEGA"
     gene            complement(9922..>10419)
                     /locus_tag="VEL68_23185"
     CDS             complement(9922..>10419)
                     /locus_tag="VEL68_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXZ38952.1"
                     /translation="RLGRHMHLKTNLIEGALYLGRVARQEIEHREFIEKEIIDLLEIE
                     PIRDKVVGMLPYGLQKRVELARALALNPKLLILDEPMAGLNLEEVEDMARFILDVNEE
                     DRWKVTCLLVEHDMGVVMDISHRVAVLNFGEKIADGTPQEVQKNALVIQAYLGEVEGL
                     YATRR"
BASE COUNT         2139 a         3113 c         2826 g         2341 t
ORIGIN      
        1 gggtgggaac gctcaaggag attggaagga cgggaccttt ctggttgcca ccagattcat
       61 caacctagtt ggtcgaagta ctggtgaatc gcgtccgccg ctttccgccc ggcacccata
      121 gccaggatga ccgtggcgga accggttacg atgtcccctc ccgcataaac cccttttttg
      181 ctcgtcgctc cggtttccgg gtcggcgatg atgtaacccc acttgttggt cttcaaatca
      241 agggttgtag aaggaatcaa gggattagca ccggttccga tggctacgac taccatatct
      301 acttccagga cgaattccga gccggggatg gggatgggcc ggcgtcgtcc ggattcatcg
      361 ggctctccca gttccatctt ttggcacttc atcccgcaga cccagccgtc ctcttttccc
      421 aggatctcca ccggcagggt caggaaatga aattttatcc cctcttcctc ggcgtggtgg
      481 acttcttcga tccgggcggg taattccttc ttggaccggc ggtagacgat gtagacttca
      541 tccgctccca ggcggagggc ggtgcgggcc gagtccatgg ccacgtttcc accgcccagg
      601 accgccactt tctttcccag ggcgatggga gtatcgtact tggggaagag ataggccttc
      661 atcaggttgg aacgggtcag gtattcgttc gccgaataaa ccccgttcag gttttcgccg
      721 gggatgttca tgaaggtcgg aagtcctgcc ccggtaccca ggaagacggc atcgtacccg
      781 ttggccatga gttcatccac cgtgaaaatc tgcccaatga ccatgtcggt cctgatctcc
      841 acccccagct tttccaggta atccacctcc gactggacga tgtctttggg aagccggaat
      901 tcggggatgc cgtaaaccaa gacaccgccg gccttatgca gggcttcgaa aaccgtcacc
      961 ttgtgcccct ttttgatcag gtccccagcc agagttaggc ctgcaggacc cgatcccaca
     1021 gccgcgactt ttttccctgt ggggggagcc atggggggca aaatgaactc tccctgcttc
     1081 tgttcgaaat ccgcggcaaa tcgctccagt cgaccgatgg ccaccggctc tcccttcttg
     1141 cccaggatgc atactttttc gcactggtct tcctgggggc agacgcggcc gcagaccgct
     1201 gggagactgt tcatttcctt gagcttccgg gccgcgcccc ggaagtcctc ctgaaggatc
     1261 agcgagatga acgccggaat gtcgatggaa accgggcatc cggccacgca ggccggtttt
     1321 ttgcattgca gacaccggct ggcttccagc ttggccagct cggggttgta accaaaaggg
     1381 acctcgtcga aattcttggc ccggaccttc ggctcttgct cgggcatggg ggtccgggga
     1441 atttgggttt tcttcttctt ttcagtcggc tcggtcatca gctctttcct ccaatgcagg
     1501 ctcccccggg gtgcaggaat atcttcgaag cctgctgctc ctgatccagg taagccctct
     1561 ggcgtttcac cagttcggca aaattcactt cgtggccgtc gaactccggc ccgtccacgc
     1621 acacgaatcg ggttttgccc ccgacgctga cgcggcagga cccgcacatc cccgtggcat
     1681 cgaccatgat ggggttcagg ctcaccacgg tcttgatgtt ccggctccga gtcacgttgc
     1741 agaccacccg catcatgggc accggtccga tggccaccac caggtggagc gggttcccct
     1801 cgtcgatgac gtcctgcaat acatcgctga cgaatccgtg atacccgtag ctcccgtcgt
     1861 ccgtggaaac tttcagcaca tgggaaacct tggccatctc ttcttccagg atcaagaggt
     1921 ccttattgcg tgctccgatg attgatatca ctctgttccc tgcgtctcgc atgcctttgg
     1981 cgatggggta gaccggagca atcccgatcc ccccaccgat gcagaccacg gtcccgaagt
     2041 tctcgatgtg ggtgggacgg cccagcggtc cgattaaatc cagaatcgaa tcgcctgcct
     2101 tgagcttgcc gagatgcatg gtggacttac ccacttcctg aaagatcagg gtaatggttc
     2161 cggcggccgg gtcgccgtcg gcaatggtca gggggatccg ttccccctct tcatgggtcc
     2221 gcagaaccac aaactggccg ggtttccttt ttttggcgat ctccggtgcg gccaccctta
     2281 attgcttcac cgaagccgcc aagagcttgg catcgacgat ggggaacact ccattttctc
     2341 ctttcccagg gatggaagaa gctcagattg aggttgcccg ccaggagggt cttgttgact
     2401 ggtcttgaag ccgccagggc agcatccact catccttcaa atggacctta ccacagccct
     2461 tgcggcattg tcaaatttcc cgctcgggca ttccaggggg taaacatcca atggatataa
     2521 ccctattttt tggggaacat cccttcaaag cctacgataa catcccctcg atccgtggcg
     2581 aagctcgcgc tcccttccga caccttgatg ccgaagggaa aatctccgat cttttcgtac
     2641 caatactgga ttaaatcggg agctttcaag ccgagcgctg ccagccgggc accgctggtg
     2701 accgccaggt ttcccggcac gacctgagag tctatgaccg ttgtcacccg taactgattg
     2761 gcggtcttcg ggggaatgta ttggctttcg tagaccatcg gggtggccag gttgaagaat
     2821 tctcccggtt ttccctgggc ttcgaaaccg acggtgaatt taatggactc cagccccact
     2881 ttgaagtcat tgggattctc cagcttgaag atgaaggcca ggatgagagg aacacggact
     2941 ttggggtcgt agggaaaata actggccacc tccacccttt ccagctccac cttgggggtt
     3001 aaaaccgctc cccccgaagc tcctttcggg gcggtggcgc aggcacccag ggccatccag
     3061 gctgccagca tgccgatgaa aataacgaaa taagttttgc gcatttccgt acctccttgg
     3121 ctaagaagtg acgccgagac gcggtaggaa atcactcccc gcgtcaccgc gtcctggttg
     3181 tcgtcgcgtc tttgttttcc gtgtctccgc ctcttctggg ttacgtcagc cagcgtttcc
     3241 gacgtttgta gtgcttgacc tgccggaaac ttttccggac gccgaggtct gtcaatccga
     3301 ggtagaaatc ccggatgtcg gggttttgaa tgaggcgttc ggaagtccca tccataacca
     3361 gtcggccgtt ttccaaaaca taggcatagg gagcgacggt cagagctaat ttcacattct
     3421 gttccacaag cagaatggga acccgttcct cccggttcaa gcgcatggtg atatcgaaga
     3481 tttccttgat cagcatgggg gccaaaccca tggaaggttc atccaggagg atcaattggg
     3541 gtctggccat catggcccgc cccatgacca gcatctgctg ttctccgccg ctgacgaatc
     3601 cggccgtcac ggcgtgaagc tgtttcaggc gggggaagaa ttggtagacc gtctccagcc
     3661 catctttcac cgtggaacta tccttgcgga gatgagcccc gacccggaga ttctcctcca
     3721 cgcttaggtg ttcgaagacc ctccgtccct cgatcacctg gatgattccc ctcttggaga
     3781 cctcttcggc gcccagccgg tcgattcgtt ctcccatgaa ctcgatcgat ccgtcggtta
     3841 cctctccatc ctcgtgtttg agcaaaccgg agatggcctt gagggttgtg ctcttcccgg
     3901 ccccgttggc gcccaggagg gcgacaatcc ccccttcggg cacctccagg gacactccct
     3961 taagcaccag aatgacctct tggtacatga cttcgatgtt gttcagcttg aggatgctgg
     4021 cttttttctc caaccggctc tccgcgtttt cctaatgact aagggggcgc ggcgtccgcc
     4081 cctacaatcc gcatttcacc ttccgaattc cgcatttgct ttaccatccc agccacgagg
     4141 gccattcgcc aggaaacttg gccttcatgt cgatttcgcc aacccccatg ggtttcccgt
     4201 ccttgattct ggccacacgg accacggaag ttgcccggtg gtcggttgga gtgtaattca
     4261 tgacttgccc gccgaattcc ttgaggctga ttcggttatt ctcaaaagcc tcccgaattc
     4321 cagggccatt cagctttccg gccttatcgg caatcttcat ggcctccacg gccagggtta
     4381 ctttgcacca cccttgtacg gtccgaatga gacgggtttc cggggaaaca ttgggactga
     4441 cctttttggc atagggtatg acgcggctcc ctttggccgg gaattcttcg cccaagaagg
     4501 cacaggccgg caggccgatc gctccctccg cggctggccc cgctagtttg accaggttct
     4561 catcgaaccc ccagatgttg atgatatggt cggccccgag tcccagggcc atggcatcct
     4621 tcagagccgt ggccaccgac atggtcgtat tcccgtgcca gactaagtgg ggtccaaaat
     4681 tcttggctgc caggacctgg ctcttcgtat ccagggcggt cagagacacg tcctgatccg
     4741 gccccacctc gaaccccagg atcttggctt gatccttgat ggccttgatc ggtgcgctgc
     4801 cgtagggaga ggcccacatg taaaaacaga caaagcgcgg tttcgttccc ttctcccgct
     4861 ccgccttcca tttggggtct ttcttccaga cttcctcgaa ccagtacgtg atcgctcccc
     4921 gggcagcggt agagtaatcg gtcccgtaga cgaagttgta gggagttttc ttggggtcac
     4981 agaggtggcc ggagaaagaa ctggacacgt agggcatctt atcggcgttg atggtcgggg
     5041 aaagggcttc cgtatccccg gtcccccacc ccaagaccat gaccaccttg tcgtagtccc
     5101 ggaatctctt gtaagtggtc accgcctccg ggacacggta cccgtagtcg tattgaaaaa
     5161 gccggatctt cttcccgttg atccctccct ggtcgttgta gtactggacc gcctctttca
     5221 cccccagggc ttcatctttc cccacgtcgg aggtggcccc ggtcaggtcc tgcagcgcgc
     5281 cgagcttgac catctgctgg gccgaagcgg gaagagcgct caatagaagc agaatcaatg
     5341 agaacaaaaa ccaatacctt ttttcgttca tctcaccctc ctcctaagaa ttcaagatcg
     5401 aggctcaaag ataagacctc atctccccat ccttaaacca cggtccatct gtctggatcc
     5461 ctagcattga acctttattc cccgtgaatc ttgaaagctt ggttcccttt tcccttgatt
     5521 tcacctcctc tctcttcggg ctaataagaa aacggccaaa ggttgaaata gttcttgatc
     5581 tgccaccatc gataggccag ccccttgggt tcaaagagga gaaagagaca gatggccaac
     5641 ccgaaggtgg attctttgat gaagagaaat ttttcctgga gctggggaaa atcctcgctc
     5701 aggggaatga cggcgaactt caggccttcc agaatcaggg tcatgaacaa agctccgaag
     5761 atcgtaccgt gggtactccc gactccgccg atcagaatga ctcccacgat ccacagagac
     5821 caactccagg taaaatgctc cgggctgaga gcagaaaggg agctcaccca gaaggccccg
     5881 gcgattccgc ccaggacgcc ggccacgaaa aaggccatca gtttgtaagc tacgatgttc
     5941 acgcccatgg tctcggcggc gatgtcgttg tcccggatgg cgatccaagc tcgcccgatc
     6001 ttggtgcgca tcaggttgga tagccccagg gtacataggg ctaccagcgc gatcatgagg
     6061 tagtaaacct tcaggtcatt atcaatggcc catgggccga gcgtgatggt acccggggga
     6121 atggaaaggg cgaccccgcg gcccccgatc tggctcacat actgggtaat gacaaagtcg
     6181 acggtaatga attgggcagc catggtggtc atgatcaagt agaagccttt taccctggcc
     6241 gatggaaggc caaagaggac gctccaaacc ccggccacca atccggccgc caccaggcta
     6301 agcaggtagg gggtctgata ctggagcatg aggatggcgc tggtgtatgc gcccacggcg
     6361 atgaaggccg aatggcccag ggtgacctgg ccgcaatacc caatcagcag ctggaccccc
     6421 atggcggaga gaattgtata tccgacgaga ctggccaccg ccagccagta gtttccggca
     6481 aacagcggga ggatgccgag aagaaagacg aagaggaaaa tcatctttcc ccggacgaac
     6541 gcggtttgcc accaggaatg ctgctggctg tatttttcat gatagacgcc gcagggaaga
     6601 aagctcataa aacccccaat gttcaatggg caataatcaa tgatcattgg tcattgctca
     6661 ttggtaattg atcattgatc ctttttacct tttcttgctg tgctgatgct ttttccaata
     6721 atggcacaca gctgttcgca ttcattgatt actggttcga cgagtttaac agcgagcatt
     6781 tcattctggt ttatcactcg cagccaatac cgcgtttctt tcagttcttt gaggacaata
     6841 ctcagtttgt gtacgaaatc ggacctagat tcgcctccgc aaccttctta ataatttgcc
     6901 cccgatgcgc tggcggatgc tacaagctgg ccggcaatat gtcttccagc aactgtttta
     6961 ggcaaggcat ccacaatttt gaggactttc ccaacaaaat ccagaagtct ctccgagagc
     7021 aggtcgcctc ttcccatcat ttttccatca tgcctttctc atttcttatt gcccattgca
     7081 tattgatcat tgcccattga tattttaaac ccgctctatt cgaacccacc cccacaaccc
     7141 gtacggtttg aataggagca cgatgaccat gatggcaaag ggagcgatct ccttgactcc
     7201 tcccggggtg aaccggctca ggtatccgtc ggccaggttc tgcaggaccc cgatgatgat
     7261 ccccccgaag atggccccgg gaaccgagtt gagacccccg aagaccaccg cgggtaagac
     7321 caggaggccc aggtatccca cggagatgtt gagcccgttg atgtttccca acagcgtgcc
     7381 gcccactccg gaagccatga aggcgatggc ccaggccagg gcgtagacga atttggcact
     7441 cactcccagg gacagggcgg ccatttcgtc atcggcggtg gcctgcatgg agagccccca
     7501 gcgggtatac ttgaagaagg ccacaaaaat caccagcagg atgatagcgg cgatgaagga
     7561 ccacagatag acctgtccga tgatgatgtt gcccaacttg attgggttta tggggaagac
     7621 tgcgggcttg aagacctgcg tgtccgttcc ccagagaaag gccacgatcc ccttgaagaa
     7681 gaaagaaagg cccacggtga ccatgatcac cgtaagcagc ggctccccga tgagccgctg
     7741 caggaagagc ctctccgtga ggagacccag gaggaagccg atgaccaagg ttcccagcag
     7801 agcaagcaag aagggaactc cccagttgta gaaggaaagg cagacatacg ccccgatgag
     7861 ggtcaactcc cccatggcca ggttcaggac ccccgagcat ttgtagatga gcgtccagcc
     7921 cagggccacc agggcataga tggagcccac catcactccg gtaatgatca gctgaaagaa
     7981 gacctccatc aggctccctc ctcggaaaca tcggccacgc gcatattggc tttgatcttc
     8041 acttccgtcc cgtcttcata ggtgatggtc gtattcatgt ggatcacgtc ctgtcgcgcg
     8101 taaaccgcct ccataatttc gcggtatcgt tgctcgacga aggcccgccg gatcttccgg
     8161 gtgcgggtca gttcgtcgtc atcggcatcg aattccttga aaaggttgac gaatcgtcgc
     8221 acccgcgccc ccttgggaag gccgttgttc atcttcacga tctctttcaa caccagatcg
     8281 tagacctggg ctttctggga gagctccggg taactggtat agggaatgtt ccggtcctcc
     8341 gcccatttgc ccaccacgct gtaatcgatg cagaccaccg ccgcgacgaa gggtcgccca
     8401 tctccgatca cccacacgtc cttgatgtag gggctgaact tgagccgggt ctccaggtac
     8461 tggggggaga atttggtgcc gtcgggaagg accatcacgt ctttggtccg gtcaaagacc
     8521 accaaatgcg cgtccctatc gatgaatccc gtatccccag aatagagcca tccgccccga
     8581 agggttttcg ccgtctccgc gggcatccgg taatatccca aaaagaccga ggggctcttg
     8641 gagatgatct ccccctccgg ggtaaccttg atctcggtcc cgggaatggg cttgcccacc
     8701 gtgtcgaact tgatgtcccc gtcccgatgc agggcagaga tgcccgcaat ctcggtctga
     8761 ccgtagattt gcttcaagtt cacccccaga gcatggaaga aacggaaatg atcgggaccc
     8821 atggccgcgc ccccggtgta ggtgtggcgg atgcgggaga ctcccaggtg gtcccggagc
     8881 tttttgtgca ccgtgaaatg ggccaggagc cccaagaccc gcagccagcc gggaacgggt
     8941 tttttggcga acttcaaatc cgcaatgcgg tagcccgcgg ccatggacca ttcatacatt
     9001 ttcctcttga tccaggtggc atcgagatat ttgacctgga cagtgcggac catctgttcg
     9061 tagattcgag gcggggagaa catgacctgc ggcccgatct cccggatgtt ctcctgggcg
     9121 gtctccggtt cttccggaaa gtttatagtg aatccggcca ggaggccgca ggagagggac
     9181 atcatctgtt ctccgatcca ggcaaaaggc aggaaagaaa cgaagtcgtc gctctcgtaa
     9241 caggggtcca cccgcatcag gttctgcccc atggtgagca tgttgtggtg gctgaggagg
     9301 gcccccttgg gaagggccgt ggttcccgaa gtgtaaaaga gaagggccac gtcgctgccc
     9361 ttccccgtcc ggaccaggac gtcaaaaagg gcgggctctt tttggtccag ctccttcccg
     9421 cgctgcacga cgttcttcaa gctgatcaaa agagggtcct cgtaccccct caagcctttg
     9481 gggtcgtccc agaccacctt ctccatcttg gggcacttgc ctttgatgga aagggccttg
     9541 tccacctctt cctgcccttc ccccatgacg aaccgggagt cggaatggtc gatgatgtag
     9601 gccacctcgt caatcagcga gtcctggaag agccacaccc cgatcccccc tgcgcatagg
     9661 gcggccatct ccaccatcaa cccctcggga cggttgtccc cgatcatggc caccttgtcc
     9721 ccccgctgca ggcccaggga atgaaggccg agggcgatcc acttcacctt ctcgtagtaa
     9781 tccttccagg tgatgggacg ccacaggccg aattccttct cccggagggc cactttgcgg
     9841 tcccagtact ttcggcaggt ctcctgaaag agcttgggga gggtgagctc cgggccgatc
     9901 tcccggtcca agtatttgag ctcatcggcg cgtggcatag aggccctcca cctctcccag
     9961 ataggcttga atcaccagcg cgtttttttg gacttcctgg ggggtgccgt cggcaatctt
    10021 ttccccgaag ttcaggaccg ccacccggtg ggaaatgtcc atgaccaccc ccatgtcgtg
    10081 ttccaccagg aggcaggtga ctttccaacg gtcctcttcg ttcacatcca ggatgaatcg
    10141 ggccatgtct tccacctctt ccaggttcaa tcccgccatg ggctcgtcca ggatgaggag
    10201 tttgggattc aaagcgaggg cccgggcaag ttccacccgt ttctgtaggc catagggcag
    10261 catgcccacc actttatcgc ggatgggctc gatttccagg aggtcgatga tctctttttc
    10321 gatgaactct cgatgttcga tctcctgccg ggcgacccgc cccaggtaga gcgccccctc
    10381 gatgaggttg gtcttcaggt gcatgtgccg acccagccg
//
LOCUS       DASPRR010000633         7140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1929152, whole genome shotgun sequence.
ACCESSION   DASPRR010000633 DASPRR010000000
VERSION     DASPRR010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929152"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1383
                     /locus_tag="VEL68_23190"
     CDS             379..1383
                     /locus_tag="VEL68_23190"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXZ38953.1"
                     /translation="MAKKLGTIVFSLLVFMGAGPVWADEIVLENGNVLTGTIEKVEGG
                     KLFLKTDYSQPVEVQVSKIKKITTDKPVDLYLTSGELVKGRIQTAPDGKITVEPESGQ
                     PPTTVDLQNVAGINTAPPKWRGSIALGGTQQTGNTQRKSASVAAEAIYKVESERFTGR
                     FLWNYARDKDDVTAENTYGLLKYDHFFTKEFYGYVAGEGWNDHFKNWRLKYNIGPGVG
                     YQIWDDEVKFLLVEGGVAYGYEDRYTGSNKDYVSARMAADIRYKIGKYLSVGDNFTIY
                     PSLTYGGQYTLRNEAYILSPLGAGWSLKLSNIWDRNSDPAPGVLKDDFMWILALQYNF
                     "
     gene            1816..2259
                     /gene="mscL"
                     /locus_tag="VEL68_23195"
     CDS             1816..2259
                     /gene="mscL"
                     /locus_tag="VEL68_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXZ38954.1"
                     /translation="MLKEFKEFAMKGNVLDMAVGIIIGAAFGPIVNSLVNDVIMPPIG
                     LLLGNVDFSNIFAVLKEGKAAGPYASLAAAKAAGAVTMNFGLFVNTIINFIIISFAVF
                     ILVKNVNRFRKEEEAAPTNKDCPFCLSSIPIKATRCPSCTSELKA"
     gene            complement(2527..3048)
                     /locus_tag="VEL68_23200"
     CDS             complement(2527..3048)
                     /locus_tag="VEL68_23200"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HXZ38955.1"
                     /translation="MNEGWLRISFGATLFLQGRFHSLLAPKGTGRGDAMGLFEALAKE
                     LLPKLLAGGGRGQSPLVDIITSLLTNPQTGGLRGLAESFKNQGMEDIINSWIGTGKNM
                     PISPDEILKALGKDRLRGYSERAGMPQEEISGGLASILPEIIDKLTPQGRIPDSSLLE
                     EELKEMKKKIAEF"
     gene            complement(3067..3369)
                     /locus_tag="VEL68_23205"
     CDS             complement(3067..3369)
                     /locus_tag="VEL68_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38956.1"
                     /translation="MKRIMMMVGLVLLIATFALAGAPKSYQVTGPVLEFDGDHITVQK
                     GNEKWEIAFDKDVKVTGGEVKKGAKVTVHYTMKASAVEIKEAAKEKPAAKEAPKKK"
     gene            complement(3455..4924)
                     /locus_tag="VEL68_23210"
     CDS             complement(3455..4924)
                     /locus_tag="VEL68_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HXZ38957.1"
                     /translation="MADQSPSLVIAMGKSEIGILPKMANRHGLIAGATGTGKTVTLRV
                     MAERFSSVGVPVFMADVKGDLSGLPFPGGDNPKVADRAKKLGLADFSPQGYPVVFWDV
                     FGEQGHPVRTTVSEMGPLLLSRILNLNDVQSGVLTIAFKIADDTGLLLLDFKDLRAMI
                     QFVGDNADQFKTQYGNISTASIGAIQRSLLALEQQGGEKFFGEPALNLQDLRQTDARG
                     KGIINIVTADKLLQSPRIYATFLLWMLSELFETLPEVGDPPKPKLVFFFDEAHLLFNE
                     LPKVLEEKIEQVVRLIRSKGIGVYFITQNPLDIPETVLGQLGNRVQHALRAFTPKDQK
                     TVKAAADTFRANPKLNVATVITELEVGEALVSVLDEKGSPTVVERALVYPPQSRLTPL
                     TPDERIKIIQGSTLYGHYEKVVDRESAFEKLRARAEEKHAGAESSLPSKGRGAAPKSD
                     VEVIVGAMAKSAAHAIGSQIGRQIIRGVLGSIFGGGRKR"
     gene            5071..5454
                     /locus_tag="VEL68_23215"
     CDS             5071..5454
                     /locus_tag="VEL68_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006033371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXZ38958.1"
                     /translation="MSRIYGMSGLLGRILISFMFLQSGINKIGGYKATLGYMESKGVP
                     GTLLPLVIFVEIAGAIAVIVGWQARLFSFLMAGFCILAALFFHMDFSNQVQTIMFMKN
                     LAIAGGFLMIFAHGAGDLSLGKKGR"
     gene            complement(5555..5815)
                     /locus_tag="VEL68_23220"
     CDS             complement(5555..5815)
                     /locus_tag="VEL68_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXZ38959.1"
                     /translation="MMTFLVQIIEIFLTIYIWMIIIRAILSWVPSFSRIPISGILFRL
                     TDPFLRKVRQFLPLPRMAVDISPVVAILVLILIRYLVVVILT"
     gene            complement(5963..6142)
                     /locus_tag="VEL68_23225"
     CDS             complement(5963..6142)
                     /locus_tag="VEL68_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38960.1"
                     /translation="MPLDLAHLVSSHAHISPVAPQFLEGIILHYADFTPADALRFSQG
                     LELFWEKAKVRGQLL"
     gene            complement(6192..6443)
                     /locus_tag="VEL68_23230"
     CDS             complement(6192..6443)
                     /locus_tag="VEL68_23230"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXZ38961.1"
                     /translation="MAVSESEERYLDSVPFSPEVSGVSLVEHIRFVLKAALFIFGQLE
                     KAHGYKIDRDLLIAAALLHDLGKIFEYRRKREFQENRHR"
BASE COUNT         1655 a         1865 c         1942 g         1678 t
ORIGIN      
        1 ttcggcccca cctcatggat gattccctgg tagccgaatt cggtgaggtt gaagatgatt
       61 ccccggccca tgttgtccgg ggaattggag accccgatca ccgccacgct ctggggatag
      121 aaaatctccc gcatgggtca cctcaaatca tgatgaaata atttttgttg ccatagccat
      181 cgggcataaa ataacctaaa gacaggggaa aggcaagccg cttggatcca aaatgtatct
      241 tcaaatgctc acaaaataca gcatttttcc cccacagata cataatgtac cctattttct
      301 ttggttcacc gaatcggata gcatagcttg attttgagat atctgcggga ctaaattcct
      361 ttgcgaaagg aggggggaat ggcgaaaaag cttgggacca ttgtattcag cctcttggtt
      421 ttcatggggg cgggccctgt ctgggccgac gaaatcgtcc tggagaatgg gaacgtcctg
      481 accgggacga tcgaaaaggt agaaggaggg aaattattcc ttaaaaccga ctattcccag
      541 ccggtcgagg tccaggtttc gaagatcaaa aaaatcacca ccgacaagcc cgtggacctg
      601 tacctgacca gcggagaatt ggtcaaggga aggattcaaa ccgcaccgga tggaaaaata
      661 acggtcgagc ctgaatccgg gcagcccccc acgaccgtcg atttacagaa tgtggccggt
      721 atcaacaccg cgccccccaa gtggcggggg agcatcgccc tggggggtac tcagcagacc
      781 gggaataccc agcgaaaatc cgcctcggtg gcggcggagg ccatttataa ggtcgagtct
      841 gagcggttca cgggccggtt cttgtggaac tatgcccggg acaaggatga tgtcaccgcg
      901 gagaatacct acggcctcct gaagtacgac catttcttca ccaaagaatt ctacggctat
      961 gtggcaggtg aaggttggaa cgaccatttt aaaaactgga gactcaagta caacatcggt
     1021 cctggcgtcg gctaccagat atgggatgat gaggtgaaat tcctcctggt ggaaggcggc
     1081 gtggcctatg gttacgagga tcgatacacc ggatcgaaca aggactatgt gtccgccagg
     1141 atggccgcgg atatccggta caaaattgga aaatatcttt cggtgggaga taactttacg
     1201 atttatccca gcctgactta cggggggcag tataccttgc ggaacgaagc gtacattctc
     1261 tccccattgg gagcgggctg gagtctaaag ctctccaaca tctgggaccg gaacagcgac
     1321 ccggcgcccg gagtcctgaa ggatgatttt atgtggatcc tggccctgca gtataatttt
     1381 taaccctctc atctccctga agctgtgaaa aaatttaggc agccggaaat agggagggcc
     1441 aggcatgccc gggggagggg caaatacacg ccgccaaaac aggcgcttgt ttctccgtgg
     1501 ccggcgtggt atttaaccca ctgaagctga agcgagaatc accttgtttt tacggaaata
     1561 ttgccaagtg gccgacgtcg ctcagaggaa gtcggggaag tgcttcacgg gcctttatct
     1621 cccgctgggt tttggcggcg tgtatttgcc cctcccccgg gcaacgagag tggattttct
     1681 ctcccttcct ctgaattttt tcgcagtttc gtttatcggc ggtgggattc tccgggaggg
     1741 gagccgagtc tagtcgggct ttataatgag acggggcgat aaccccacaa catccatcca
     1801 tgaaaggagg gtattatgtt aaaggaattt aaggaattcg ccatgaaggg aaacgtcctg
     1861 gacatggccg tgggtatcat catcggggcg gccttcgggc ccatcgtgaa ttccctggtg
     1921 aacgatgtga tcatgccccc catcggcctg ctcctgggga acgtggattt ctccaacatc
     1981 tttgccgttc tgaaggaggg aaaagcggcg ggcccctacg cctccctggc cgccgcgaaa
     2041 gcagccgggg ccgtgaccat gaattttggc ctctttgtca acacgatcat caatttcatc
     2101 atcatctcct ttgccgtctt tattctggtc aagaacgtca accgattccg gaaggaggaa
     2161 gaagcagccc ccacgaataa ggactgcccg ttctgccttt ccagcattcc gatcaaagcg
     2221 acccgctgcc ccagctgcac ttcggaattg aaagcatagg gcataggaaa aaaggatcga
     2281 ggaacgaggt tcgaggtttg aggaatatgt gctgagccct aaggcaaata cacagggcac
     2341 agaaggcaaa acaaaccaac cgttcaatca aaagggggac cgagatttta tcccaggtcc
     2401 ccctttttct ttccttgtgt cggatagggc caatacttac ttcaccgtga tatcgtcttt
     2461 aatctttcca tagattcctt ctttaatcgc cccgtcgttt gatcttaacc cctttcacct
     2521 cctgaatcag aattcggcga tctttttctt catctctttc agttcttctt ccagtaagct
     2581 gctgtccgga atcctgccct gcggggttag cttgtcgatg atctccggca aaatgctggc
     2641 cagccctcca gagatttcct cctgaggcat tcccgccctt tccgaatatc cccggagccg
     2701 atctttgccc agggccttca gaatctcatc cggggaaatg ggcatattct ttcccgtacc
     2761 gatccaggag ttgatgatat cctccattcc ctggttcttg aaggattccg ccagccctct
     2821 gaggccgccg gtttggggat tggtcagcaa gcttgtgata atatccacaa gcggactttg
     2881 gccccttcct ccgccggcaa gaagtttcgg aagtaactcc ttggcaagag cttcgaacaa
     2941 tcccatggcg tccccccttc cagtgccctt cggagcaaga agactatgaa atctcccctg
     3001 aagaaaaagg gtggctccga atgaaatccg aagccaccct tcgtttattc cccgggtatt
     3061 ttgtggctat ttctttttgg gggcttcctt ggcggcgggt ttttctttgg ccgcttcttt
     3121 gatctctacc gccgaggcct tcatggtgta atggacggtg accttggctc ccttcttcac
     3181 ttctccgcca gttaccttga catctttgtc aaaggcgatt tcccactttt cgtttccctt
     3241 ctgaacggtg atgtgatcgc cgtcaaactc cagcaccggg cccgtcacct ggtagctctt
     3301 gggggcgccc gccagggcaa aggtagcaat cagcagaaca agtccaacca tcatcatgat
     3361 ccgtttcata aatgccctcc ttttttggaa tttgtaaaat gataaaagaa tcggagaaaa
     3421 aagtaaatac gtattttcgc ccgtttttgg ccctctagcg ctttcgtccc ccgccaaaaa
     3481 tcgaccccag gaccccccga ataatctgcc ttccgatctg gcttccgatg gcatgggcgg
     3541 cactcttggc catggctccg acaatcactt ccacatctga tttgggggct gcaccacgcc
     3601 ccttggaggg aagagaggat tccgccccgg catgtttctc ctccgcccgg gctctgagct
     3661 tttcaaaggc cgactcccgg tccacaacct tctcataatg gccgtaaaga gtggacccct
     3721 ggatgatctt aatgcgttcg tccggggtga ggggggtcag acggctctgg ggaggataga
     3781 ccagggctcg ctccacaact gtaggagatc ctttttcatc cagcacagag accaaagcct
     3841 cgccgacctc gagttcggta atcaccgtcg ccacattgag cttgggattg gcccggaagg
     3901 tatcggcagc ggccttcacc gtcttctggt cctttggagt gaaagcccgc agggcatgct
     3961 ggacccggtt tccgagctgg cccaggacag tttccggtat atccaggggg ttctgggtga
     4021 tgaaatagac cccaattcct ttggaccgga tgaggcggac gacctgctcg atcttctcct
     4081 ccaatacctt ggggagttca ttaaacagga ggtgggcttc gtcaaagaag aagacgagtt
     4141 tgggtttggg tgggtctcca acctcgggaa gggtctcgaa gagttcggag agcatccaga
     4201 ggaggaaggt ggcatagatc ctgggagact gaagcagctt atccgccgtt acgatgttga
     4261 tgatcccctt tccccgggcg tcggtctgcc tgagatcctg caagttgagg gcgggttcac
     4321 cgaagaattt ctccccccct tgctgctcga gggccagcag gcttcgctgg atggcgccga
     4381 tactggcggt ggagatgttc ccgtactggg tcttgaattg atcggcgttg tcccccacga
     4441 actggatcat ggcccgaagg tccttgaagt cgaggaggag gaggccggtg tcgtcggcga
     4501 tcttgaaggc aatggtgaga accccgctct ggacgtcgtt gagattgagg atgcggctga
     4561 gaagcagagg gcccatctcc gatacggtcg tgcggacggg atggccctgt tctccgaaga
     4621 cgtcccagaa taccaccggg tagccctggg gggaaaaatc ggcgagccca agcttcttgg
     4681 cccggtccgc tactttggga ttatcccctc ccggaaaggg caagccggaa aggtcccctt
     4741 tcacgtcggc catgaagacc ggaacgccga cgctgctgaa ccgctcggcc atgactcgca
     4801 gggtcacggt cttccccgtg cccgtggccc cagcaatgag gccatggcgg ttggccattt
     4861 tgggaaggat cccaatttcg ctctttccca tagcgattac cagagacgga gattggtcag
     4921 ccattttgcc ctcctcaaag ggtataaaac tgggttaata tatataggat tgccgaacct
     4981 gtcaacggac ttgagaaatt cggcgttcgg ggtgctaaac accgggcata tcgatgattt
     5041 aaccgcccgg gcggaatgaa aggagggacc atgtcacgaa tctacggaat gagcggatta
     5101 ctgggacgaa tcttgatctc tttcatgttc ctgcaatcag gcatcaacaa gatcgggggg
     5161 tacaaggcga ccctgggcta catggaatcg aagggggtgc cggggacgct gctgcccctg
     5221 gtcatcttcg tggagatcgc cggcgccatc gcggtcatcg ttggctggca ggcccgcctg
     5281 ttctctttcc tcatggctgg cttttgcatc ctggccgcgc tgttttttca tatggatttc
     5341 tcgaaccagg ttcaaaccat catgttcatg aagaaccttg ccatcgccgg gggattcctg
     5401 atgatctttg cccacggggc gggagattta agcctgggca agaagggcag gtaaaagcgg
     5461 aagaaaatcc cgattcagcc cattctccgg gtttccgctt tgcatttgtg acccaataat
     5521 tcaagacttg aatttgtcga atcggctccg gacactaggt taaaataacc accaccaggt
     5581 agcggatgag gataagaacc aggatggcta cgaccggaga tatgtcgacg gccatcctcg
     5641 ggaggggcag gaattgccgg accttcctca ggaaagggtc ggttagacga aagaggatac
     5701 cggagatggg aattcgggaa aaggagggaa cccacgatag aatggctcgg atgataatca
     5761 tccagatgta gatggtgagg aaaatttcga ttatctgaac taagaaggtc atcattaaat
     5821 ctcctcgcga tccgtaccgg ggaaaaaagt cgggtccaat aagtgggggc aggtaatccc
     5881 cgggattcga tccgccctaa ccattacccc atcccctaat ctggccgcta tccctcatag
     5941 cccatgaccc tttacctatt acctacaaca gttgacccct aacctttgct ttctcccaga
     6001 aaagctccaa accctggctg aaccgcaggg cgtcggcagg ggtaaaatca gcatagtgga
     6061 ggatgatccc ttccaggaac tgaggagcga cgggagagat gtgagcatga ctggagacca
     6121 agtgggccaa gtccaggggc aacccctccc gtaaggtcag aaaggttccc cagaatccat
     6181 gggggaagaa tttaccgatg tctgttttcc tgaaattccc tcttccggcg gtattcgaag
     6241 attttgccca aatcatggag aagagcggcg gcgatcagaa ggtctcggtc gatcttgtac
     6301 ccatgggcct tctccaattg gccgaagatg aaaagagcgg ccttcagcac aaatcggata
     6361 tgctcgacga gggagacccc gctgacttcc gggctgaaag gaacggaatc aaggtatctt
     6421 tcttcgctct ctgagacggc caagttccag accttcggca ctccttggcg caggccaggt
     6481 gactgaatct gctttatttc cgggaagatg gaaagaaagt aggtttttat tcggtctttt
     6541 tctttggcat tctcttagct ctccttcgtt caggtaatat ataggacacg gtgcaagcag
     6601 gtcaaataaa atttatggag ggcgacttca aagagtttat ttcctgacct gttttcctgt
     6661 cctctccttg actctttatg cggatggcta tctccaggca ttcccgaaga tgcaaaaatt
     6721 atcgaccaat ttcgataagc ggagaaggaa ttctcccggc atatcttttg cttcttcttt
     6781 ttatagaaga atggggaagg gtgaagaaaa catccggtca gcccctcggc cgtttggctt
     6841 ttacccttgg aagggagtga aagagagtgg ggaggaggcc ttcccaaagc aaggaccgat
     6901 attccggggg acttttatac ttctgagatt tatggccgat gtatatttaa gaaaccaaca
     6961 acggtgatga ggaggcattc cttgctatga catttcagga ggcttttcag gtagggaaga
     7021 agtatctgcg atttcccaca ggacggaagg cccccctcca gaatcagcat cccccatcca
     7081 agagcgtaat gccgggagga tacacgggtc atccgggagg cggaccttac tccatggcca
//
LOCUS       DASPRR010000634         4504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1284060, whole genome shotgun sequence.
ACCESSION   DASPRR010000634 DASPRR010000000
VERSION     DASPRR010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284060"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VEL68_23235"
     CDS             <1..412
                     /locus_tag="VEL68_23235"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXZ38962.1"
                     /translation="YINDALLQGDIKTTLSQTGILFLLRQEDGQSMTQLSNALDIDNS
                     TLTGLIDRMERSGYVSRRPGDSDRRAFRICSTPQGLEESNRAKPLIRKINEEIKSGFS
                     QEEIETFKRVLTGLLEKFGKSRARRTRRQKGEHR"
     gene            767..2152
                     /locus_tag="VEL68_23240"
     CDS             767..2152
                     /locus_tag="VEL68_23240"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HXZ38963.1"
                     /translation="MAVIVPFRGVSYNPKKIDDLSKVVAPPYDVISPQEQDALYQRHP
                     QNVVRLILNKETPQDHPKDNRYTRSAALYRGWQKAGVLVRASRPQFYFLQEEFSPSIL
                     PAGQTVSISGTIVRQGFIGLIQLEEYSAKVVLPHEKTQTKPRADRLALMEACQANFSQ
                     IYSLYCDEKGVMGPIYKQAFSSGPPVFDVTDTEGVRRKLWMIGEPGILKQVGENMKSK
                     EIYIADGHHRYETALAYREKQRQKFPKSTGRETYNFTMMYFAAMEDPGVFILPTHRVV
                     SNLENFEASSFLDKLRPDFMIEAFEFQEGNDKAVRDRLLQQLAFRPEKERVLGMLLKG
                     LKKYYLLTLKDNRTIKLVEPGIAPSLEALDVNLLHIPILKKRLNIGAQDLAASKKVIY
                     FKDPGDAAAAVQSGDGQIAFFLNPTRTYQVRDVSLAGETMPSKSTFFYPKLLSGLVIN
                     PLDPHEEIAVQ"
     gene            2130..2870
                     /locus_tag="VEL68_23245"
     CDS             2130..2870
                     /locus_tag="VEL68_23245"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HXZ38964.1"
                     /translation="MKRLPCNESEEALDTLFGGRLKVLQKKRGYRFSIDALLLAHFAE
                     PDPDDRIIDLGTGCGIVPLILFFRKKSKKITGVEVQPSLADLARRNAVLNRCSSRFQV
                     WEDDLRKLDQRVKRESFDLVLTNPPYRRVGSGRVNPHTEKAIARHEIQATLEEVLQAT
                     HYLLKDKGRLVMIYPASRVADLMRGLSKCHLEPKRVQFVHSHENDEARLVLVEAHKEG
                     HAQVKILPPFYLFEPSGDYTPAARELFE"
     gene            3145..3828
                     /locus_tag="VEL68_23250"
     CDS             3145..3828
                     /locus_tag="VEL68_23250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXZ38965.1"
                     /translation="MFIPLKDDNPTLTFPIVTIGLILANGWVFFHQISLDVVESQRFI
                     FQWGAIPYQIVTGESLYGPSRISVYLTLFSSMFLHGGFLHLFGNMLYLWIFGNNIEDT
                     LGHFRFLIFYLVCGLSAALAQVLSSPSSTVPMIGASGAVAGILGAYLLLFPGARILTL
                     FFIFIIIKFIRLPAIIVLGFWFFLQLMGVMGGAISNVAFFAHIGGFVVGLVLIKIFQP
                     RWGKRRRKY"
     gene            4059..>4504
                     /locus_tag="VEL68_23255"
     CDS             4059..>4504
                     /locus_tag="VEL68_23255"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXZ38966.1"
                     /translation="MPDEIIYGINPILEALRSERQGLNKILLAEGKEGGPIRLLRQLA
                     REKGIPVQVSPKEALDRLAGSGHHQGILGFTAASSYSTWEDLLDRVRSAQGKAIVLVL
                     DSIEDPQNLGSLIRTAEACGVKGVILPKDRAAGITPAVVKASAGAVA"
BASE COUNT         1073 a         1204 c         1166 g         1061 t
ORIGIN      
        1 ttatatcaat gacgcccttt tgcaagggga cattaaaacc accctgagcc aaaccggcat
       61 cctcttccta ttgagacaag aggacggaca gagcatgacc caactgagca atgctcttga
      121 cattgacaac tccaccctga ccggcctcat tgatcgaatg gagcgatccg gctatgtgag
      181 ccgaaggccg ggtgattcgg accggcgagc attccgcatt tgcagcaccc ctcaggggct
      241 ggaagaaagc aaccgggcca aacctctgat cagaaagatc aacgaggaga tcaagtcggg
      301 gttttcccag gaggaaatcg aaaccttcaa acgcgtcctg actggccttc tcgaaaaatt
      361 cggtaaatcg agggcgagaa gaacaaggag gcaaaaaggg gaacaccgct gaattggcgg
      421 gataagggta gtaatgcata accaaggaca ggaactgcag aacaagttcc gcggaattgc
      481 tctatcaatg gatgactttt taggatcccc agccctcgga agtgttcgga agagggaagc
      541 aaggtatcga acacgtgttc cacgctgagt tgaaggactc agccaggagc ccctgtgcag
      601 atggtcccct atctcttatg tctattcgcc tctgcgattg actaaatggc ccacccaggt
      661 tcagttgaag agccgaaggc cctcgaaaga attgtaagga gccttgtttg gaacccttcc
      721 ttcgtgctat aaatgcttac cagccaatga aaggagaccg agaaaaatgg cggttattgt
      781 gccctttcgg ggggtcagct acaatcccaa aaagatcgat gacttgagca aggtggtggc
      841 gcctccctac gatgtgatct ctccgcagga gcaggacgct ctctatcaac ggcatcctca
      901 gaatgttgtt cgtttgatcc tgaacaagga aacgccgcag gatcatccga aagacaaccg
      961 ttatacccgt tcggctgccc tttaccgagg ctggcagaag gcaggagtcc tggtccgggc
     1021 ctccaggccc caattctatt ttttgcagga agaattcagc ccttccattc ttcctgccgg
     1081 ccaaacggtc tccatctccg ggactatcgt tcgccagggc ttcatcgggc tgatccaatt
     1141 ggaagaatac agcgccaaag tcgttctgcc ccacgaaaag acccagacga agccgcgggc
     1201 cgatcggctg gccctgatgg aggcttgcca ggccaatttc agccagattt attccctgta
     1261 ttgcgacgaa aaaggagtca tggggccaat ctataagcaa gctttctcct ccggtccccc
     1321 tgttttcgac gtgaccgaca ctgaaggagt ccgccggaaa ctctggatga tcggcgaacc
     1381 gggaatcctt aagcaggtcg gggagaatat gaagtccaaa gagatttaca ttgccgacgg
     1441 gcaccatcgc tatgaaacgg ctctggccta ccgcgaaaag cagcggcaga agtttccgaa
     1501 gagtacgggc cgggagactt acaacttcac catgatgtac tttgcagcca tggaagaccc
     1561 gggagtcttc attcttccaa cccaccgggt ggtgagcaac ctggagaatt tcgaagcttc
     1621 ttcttttttg gataagctac ggcccgactt catgatagaa gccttcgaat tccaagaagg
     1681 gaacgataaa gcagtccggg accggctcct gcagcaactc gcctttcgcc cggaaaagga
     1741 acgcgtcctg ggaatgctgt tgaagggcct aaaaaaatac tacctcctta ccctgaaaga
     1801 caatcgcacc atcaaactgg tggaaccggg catcgcccct tcgctggagg ccttggacgt
     1861 gaaccttctc cacattccca tcctgaagaa acgtttgaac atcggtgcgc aggatttggc
     1921 ggccagcaag aaggtaatct atttcaagga cccgggggat gcggccgcgg cggtgcaatc
     1981 gggagacggg cagatcgcct tcttccttaa ccccactcga acctatcagg tacgggacgt
     2041 gtccctggcc ggagagacca tgccctccaa atccaccttc ttctatccca aactgctgag
     2101 cgggctggtc atcaatcctt tggaccccca tgaagagatt gccgtgcaat gaatcggaag
     2161 aagccctcga caccctcttc gggggccggc tcaaggtcct ccagaagaag cggggctacc
     2221 gtttctccat cgatgccctg cttctcgcgc attttgccga accagacccg gacgatcgga
     2281 tcattgattt agggaccggc tgcgggatcg ttcccctgat tctcttcttc cggaagaaat
     2341 caaaaaagat cacgggcgtt gaggtccaac cctccctggc tgatctggcc cgcaggaacg
     2401 cggttctgaa ccggtgctcc tcccgattcc aggtctggga agatgacctg cggaagctgg
     2461 accaaagagt aaagagagaa agcttcgacc tggttttaac aaaccctcct taccgtagag
     2521 tcgggtccgg ccgggtgaac ccccacacgg aaaaagccat tgcgcgccac gaaattcagg
     2581 caaccttgga agaagtgctg caggccactc attatttatt aaaagacaag gggcgcctgg
     2641 tgatgatcta tcctgcctcc cgggtcgctg acttaatgag agggctgagc aaatgtcacc
     2701 tggaacccaa gcgcgtccag ttcgtgcatt cccatgaaaa tgacgaggcg cggcttgttt
     2761 tggtggaagc ccacaaagaa ggccatgccc aagtgaaaat ccttcctccc ttctatctct
     2821 tcgaaccttc gggggactat actcctgctg cccgggaact ctttgaatga ttgtggagtt
     2881 gattaaggat gatagagggc ggattgagga ggaaggacga tagttatttg aaatttcctg
     2941 ccagatatcg gataattcag atgttcatga gcttcttgcc aatgtcgccc tttccgtcat
     3001 ttcggcgtaa gccgggatcc aggattcttt gaaaatcctg gatcccgggt cgcgtttcgc
     3061 ttgtccggaa tgacgacttc ttgtttccgg caagagtttc gcaagaagct ttatttatgc
     3121 aggttgaaaa aagggacacc atccttgttt atccccctta aagacgacaa tcccaccttg
     3181 acctttccta tcgtcaccat cgggcttatt ctggccaatg gatgggtctt ttttcaccag
     3241 atttcgctgg atgtagtcga aagccaaagg ttcatcttcc agtggggggc gattccgtac
     3301 cagatcgtta cgggggagtc tctttacgga ccctccagga tttccgttta tctcacccta
     3361 ttttcctcca tgtttctcca tggtggattc cttcacctct tcggtaatat gctttatctt
     3421 tggatcttcg gcaacaacat cgaagacacc ctgggccatt tccgtttcct cattttctac
     3481 ctggtctgcg gtctttctgc tgccctcgcc caggtcctct ccagcccaag ctcaaccgtg
     3541 cctatgatcg gcgccagcgg ggcggtggcc ggaatcttgg gagcctacct cctccttttc
     3601 cccggagccc ggattcttac cctcttcttc atctttatca tcataaaatt catccgcctt
     3661 ccagcgatca tcgtgctggg cttctggttc ttcctgcagc ttatgggagt tatggggggg
     3721 gccatttcca atgtggcctt ctttgcccat attgggggat tcgtcgtagg gctggtcctg
     3781 atcaaaatct tccagccccg ttggggcaaa cgaaggcgca agtattgaat ttttttacca
     3841 tagaggcaca gagaaaacag agttagaatt aattggggga tcaataaaat ttcaaataca
     3901 aagcctgaat tttcaggcaa atattaatga atcaaattcc gttacgcaca attatatttc
     3961 agaaatagct caatacgggc cattcagaaa tttggatgtt ttaaagattt gattttggct
     4021 tctctgtgtt ctctgtgtcc ctgtagtgaa tatttcttat gcctgacgag atcatttacg
     4081 gaattaatcc gatcctcgaa gctttgcggt ctgaacggca gggtctgaac aagatcctcc
     4141 tggctgaagg gaaggagggc ggacccatac gtctgcttcg ccagctggcc cgagagaagg
     4201 ggattccggt tcaggtttca cccaaagagg cccttgaccg gctggccggg agcggacacc
     4261 accaggggat tctgggcttt acggccgcgt catcttattc cacgtgggag gatttacttg
     4321 acagggttcg gtcagctcag gggaaggcca ttgtcctggt cctagatagt atcgaagacc
     4381 cgcagaacct cgggtctcta atccggaccg ccgaggcctg tggggtcaag ggagtcattc
     4441 tccccaaaga ccgcgccgcg ggcattactc cggcagtagt caaagcttct gccggggctg
     4501 tagc
//
LOCUS       DASPRR010000635         8041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_2406079, whole genome shotgun sequence.
ACCESSION   DASPRR010000635 DASPRR010000000
VERSION     DASPRR010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406079"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VEL68_23260"
     CDS             complement(<1..519)
                     /locus_tag="VEL68_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ38967.1"
                     /translation="MGTKRKARFRPAYLDLYEKGALIGRAAEAQSRLESCQLCPRECK
                     VNRQADQKGFCRTGLRAVVSSHSPHFGEEDPLVGTGGSGTIFFSHCNLLCLFCQNYEI
                     SHLGEGQEVSPDQLAQMMLHLQALGCHNINFVSPSHVVAQILAALPVAVEGGLRVPLV
                     YNTGGYDSVETLR"
     gene            complement(523..2238)
                     /locus_tag="VEL68_23265"
     CDS             complement(523..2238)
                     /locus_tag="VEL68_23265"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ38968.1"
                     /translation="MKEEEGSQIREQENGQFIERVGCFRRSPKGKVSPRVKTDIFGAV
                     AESLGAMANSSGGTVLLGAASEEEVRGVFWDERGQRLFINLLREAFLPPVAFEVVSQE
                     VLGKTLLRFTVSPSPAIHTQKNGETHLRVGAQNISLSRERLAALREARTETWHEREIL
                     PRSSWSDLDSDLVEEFLSHLGLRGEAEKTLHRPYGLVEYIENRPVLTRAAVYLFGRDP
                     LRWHPRSGIEFVRFEGTEEKSREGYNVAERTRIEGPLLRLIREAEKSIGERIREQVLQ
                     RDLFFRERFELPSFVWREALINALAHRDYSLEGIGISVWMFDDRIEIHSPGRLPRTVK
                     AGQILRRQRVHYARNPLITRVLSDAGYMRARGEGLPRVFQEMDRHGLNPPDWREEANG
                     CCLVFRNTPVLDEAALVWLKQVNQISLNPRQKRILAYARVHGGIFSSSDYQKFGVDRD
                     GAYVEIKALVRQGLVEPMKKHGKVYRVREIQEEKTTLPGLAWVLGPLKAKGYFTLKEL
                     ELPPSISRKKAWTLVRNLAKEGYFSPSGRGRATRYHLTEKLKPHLEKMDFPFSFPAEG
                     ERTEN"
     gene            complement(2249..3013)
                     /locus_tag="VEL68_23270"
     CDS             complement(2249..3013)
                     /locus_tag="VEL68_23270"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXZ38969.1"
                     /translation="MEERNNPLFKLIDTHAHLDEVQDPEEALKRAQDAGVQAVVGVGT
                     DLPSNEKILHLASRFPNFVLPAVGFHPWRLEGIDAAANIRFIELEIPRCVALGEVGLD
                     FALKTPREKQEEVLQELLKIALRKKKPALLHARRAWAEALRILKMRPVDRAIFHWYSG
                     PLDILQQVFAQGYWISATPAVAYSAKHRLAIQAAPLDQLVLETDTPEPYLGIKSEPKD
                     LLKTLQSVSELKGRSPEEIARHTYRNSLAFFRLAGS"
     gene            3282..4352
                     /locus_tag="VEL68_23275"
     CDS             3282..4352
                     /locus_tag="VEL68_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HXZ38970.1"
                     /translation="MRPKSFEFLKALAEAPSPSGYEQPAQKVFREYIQGCADEVRTDV
                     LGNTFGFIRGSKTKPVVMLSGHCDEIGFMVTYIDENGFAFFSPIGGVDTQLLPGKRVW
                     VHTAKGSRVGVMGRKAIHLIDPKDREKVVQIHNLFIDFGAKDKKEAESLVKIGDPITF
                     AVGLERLQGDRVVSRGFDDKMGSFVVGEVLRRIWERKERPRCSVYGVSTVQEEIGLRG
                     ATTSAYQVQPDVGIAVEVGHASDYPEVDKKRVGEYKLGAGPIIARGANINPAVFALLQ
                     KAAKRGKIPIQILGAPRATGTDANVIQLSRKGVATGLVSVPLRYMHTPAEVLSLKDLE
                     ATCDLLVEFIHLLREDMSFIPG"
     gene            complement(4434..5816)
                     /locus_tag="VEL68_23280"
     CDS             complement(4434..5816)
                     /locus_tag="VEL68_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXZ38971.1"
                     /translation="MKGTPKFQVLIVDDEQVQREMLEGFLGKQGYGVETAGSGAQALD
                     SYKGSSFDLVLSDYRMPGMDGIQLLKELKRLNPEVVVVILTAFGTVGTAVAAMKEGAY
                     DYLTKPIDLDELLLLIQRAERELGLRRENRDLRDQLREKFKVDFIISASRGMEEALNL
                     VKRAAPSAATVLILGESGTGKELIARAIHYSSPRADRPLVKVNCAALPDNLLESELFG
                     HEKGAFTGAVSRRIGRFEQADQGTIFLDEIGDLSSSLQSKLLRVLQEKEFERVGSSQT
                     LKVDVRVVAATNRDLEEEIQKGTFREDLYYRLNVVTIFLPPLRERKEDLPPLIEHFLK
                     KYTRENQKSMLSLSKEARDLLLNYNYPGNIRELENIVERAVVLSRGDTIMTEDLPLNL
                     REGKVEDLMKEAGKRRSLPETLEEIERLRITQALESSHSVQTKAAEELGISERVLRYK
                     MKKYGIGEKD"
     gene            complement(5813..7579)
                     /locus_tag="VEL68_23285"
     CDS             complement(5813..7579)
                     /locus_tag="VEL68_23285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXZ38972.1"
                     /translation="MAFLPKGPERGKRWVFRPLFLLIIWGLLALVLLINGIYETKRLK
                     DNLHRLLYDEGAALIAGLEKNAQTSFASVTALETFPEASALLIPSPVNPLALDDSVVD
                     LLLDLAFQMDRQIGSRAPEEKDLAGMAKTWQLSGITFISPRQTVTYPQSGEGLRGGDP
                     FIRPLLEGRTAYAIQRSEKKETGQMDHLSVAIVRRAGEGILAVRADERSIRNLRRRAL
                     LQGLIEEWKGRGEIRYIIFQGEDLEVWADTAPERIGRKEESGFLRHLPVGETREAMAR
                     ELGEEKILELAKRVVLDADSRGILRVGLSTARVDEILKNDQKNLLLFSLFLLVFGGAA
                     IVLISRMENRHLARVREMEEKVRQSERLSSLANLAAGVAHEIRNPLNAIGMAIQRLQR
                     EFAPTVPEAQKEYLRFTDVLRGEVKRVNQIIEQFLFFARPARLDLQSVQIREVLRDLL
                     LLARETAERQNVQLIESLAGEFPRLNLDRQRIHEALWNLVNNALQAMPQGGNLEIGAR
                     YNSPSREVIVRVSDTGAGIAEENLGRIFDYYFTTKEKGMGLGLPLAYKIIRDHGGAIE
                     VDSVAGKGTAFRVTLPVPGEER"
     gene            complement(7598..>8041)
                     /locus_tag="VEL68_23290"
     CDS             complement(7598..>8041)
                     /locus_tag="VEL68_23290"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXZ38973.1"
                     /translation="QAAPAQGPPWRKGWEDRGDRGVMGPMHAPMMDWASQLNLTADQS
                     SKLQELRETYLRDTLVWRNELVIKRFDIRDLLRNPQSDSNTILAKQKEIFDLEAKIQE
                     RGLLLLIEMRKVLTPEQIKLLPPHWGGIYGPPMMPWRGRGMEKEY"
BASE COUNT         1679 a         2340 c         2234 g         1788 t
ORIGIN      
        1 acggagggtt tccacagagt cgtaaccacc ggtattgtaa accaggggaa ccctcaagcc
       61 accctcaacg gcgacgggca gggcggcgag aatctgggcc actacatggg atggagagac
      121 aaagttgatg ttgtggcatc ccagtgcctg tagatggagc atcatctggg ccagttggtc
      181 aggggaaact tcctgacctt cacccaaatg gctgatctca taattctggc agaaaaggca
      241 gagaagattg cagtgactga aaaaaatggt ccccgacccc ccagttccca ccagagggtc
      301 ctcttcgccg aaatgggggc tgtggctgga aaccacggcc ctcaggcctg tccggcagaa
      361 tcccttttgg tcagcctgcc ggttcacctt gcactcccgc gggcagagct ggcaggattc
      421 caggcggctc tgagcttccg ccgcacgccc gattaaggca cccttctcat acaggtccag
      481 ataagcagga cggaatcggg ctttcctttt tgtccccata cctcagttct ccgtcctttc
      541 cccttcggct gggaatgaaa aggggaaatc catcttttcc aggtggggct taagtttttc
      601 agtcaaatga tagcgggtcg cccttcccct ccccgaagga ctgaagtatc cctccttggc
      661 cagattgcgg accagggtcc atgctttttt ccgcgaaatg gacgggggga gttctaactc
      721 cttgagggta aaataccctt ttgcttttaa gggccctagg acccaggcta acccgggcag
      781 agtggttttt tcttcctgaa tctcccgaac ccggtagact ttcccatgct tcttcatggg
      841 ctccaccaac ccctggcgta ccagggcctt aatctccaca taggcgccat cccgatccac
      901 cccgaacttc tggtaatcgg agctgctgaa aattccccca tgaacccggg cgtaggccag
      961 gatgcgcttt tgccgcgggt tcaaagaaat ttggttaacc tgtttcaacc aaacaagagc
     1021 cgcctcatcc aagacgggag tatttcggaa aacgagacag cagccattcg cttcctcccg
     1081 ccaatccgga ggattcaacc cgtgtcggtc catttcctgg aagacccgag gaagtccctc
     1141 cccccgggcc cgcatatagc cggcatcgga caagacccgg gtgatcaggg gattcctggc
     1201 ataatggact ctttggcgcc gtaggatttg acccgccttg accgtccggg ggagacggcc
     1261 ggggctgtga atctcaatcc ggtcgtcaaa catccagacc gaaataccga tcccttccag
     1321 agagtagtcc cggtgggcca gagcattgat cagtgcctca cgccagacga aggatggaag
     1381 ttcaaacctt tccctgaaga agaggtctct ctgcagtact tgttccctga tcctctctcc
     1441 gatgcttttt tccgcttccc ggatcagtcg caggaggggc ccttcgatcc gagtccgctc
     1501 ggctacgttg tatccttcac ggcttttttc ctctgtcccc tcaaagcgta caaactcgat
     1561 ccccgaccgg ggatgccagc gaagagggtc ccggccgaag aggtagaccg ccgcgcgggt
     1621 cagaactggc cggttttcta tatactcaac caatccgtaa ggacggtgga gggtcttttc
     1681 cgcttcaccc cgaaggccca ggtgggatag gaactcttcc accaagtcgg aatccagatc
     1741 agaccatgaa ctccggggga gaatctccct ttcatgccaa gtctcggttc gggcttcccg
     1801 tagagccgcc aatctttccc tggagaggga tatgttctgc gcgccgaccc gaaggtgggt
     1861 ttctccgttt ttctgggtgt ggatggcggg gctgggagag acggtgaagc gaagcagggt
     1921 ctttcccagg acctcctggg aaaccacttc aaaggcgaca ggtgggagaa aggcctcacg
     1981 aagaaggttt ataaagagcc gctggccccg ttcatcccaa aagacccccc ggacttcctc
     2041 ttcggaagca gcgcccaata gaaccgttcc cccgctggaa ttggccatgg cccccaggga
     2101 ttcggccacg gccccgaaga tgtctgtctt cacccgggga gaaacctttc ccttcgggct
     2161 gcggcgaaaa cagcccaccc tctcgatgaa ctggccgttt tcttgttccc ggatttgaga
     2221 cccctcttct tctttcataa agcccttctt aagagcccgc aagccggaag aaggccaagg
     2281 aattgcggta ggtatgccga gcaatctcct caggagatcg gcctttcagc tcactgaccg
     2341 actgcagggt tttcaagagg tccttcggct cgctcttaat tcccaggtaa ggctcggggg
     2401 tatcggtttc cagcacgagt tgatccaagg gagcggcctg gatagccaaa cgatgtttgg
     2461 cgctgtaagc cactgccggg gtagcagaga tccaataccc ttgagcaaag acctgctgta
     2521 gaatgtccag gggaccgctg taccagtgaa atatcgcccg gtcgaccggc cgcattttca
     2581 ggattcgcaa ggcttccgcc cacgctctgc gggcatgaag aagggccggt tttttcttgc
     2641 gcaaagcgat tttgagaagc tcctgcagaa cctcttcctg tttttccctg ggggtcttga
     2701 gggcaaaatc aagacccacc tctcccaagg ccacgcagcg aggaatttcc agttcgataa
     2761 aacggatatt tgctgcggca tcgatccctt ccaaacgcca gggatgaaaa cccacggccg
     2821 gaaggacgaa gttgggaaaa cgactcgcca ggtggaggat tttttcattc gatggcaggt
     2881 ccgtcccaac cccgacgacc gcctgaaccc cagcatcctg ggcccttttc agggcttctt
     2941 cggggtcttg gacctcatcc aggtgagcat gggtatcgat gagtttgaat aatggattgt
     3001 tcctctcttc catgctgccc cattatcgcc aaccgcagga gaccggtcaa ggagattttg
     3061 attttcggaa actgattatc ctcagattac gcagactggg aatagggggc aaagaagcag
     3121 aaaagaatgg ggatagatct tgaatcgccc gcttggccct cctgcccggg aatataatca
     3181 gaaccccgca gaagggactt gaacaaaggc tgaaggatgg gatactttaa tagagagata
     3241 aaaaaatttt ggggcaggaa ctcatcatca caggaggaaa agtgagaccc aaatcctttg
     3301 aatttctgaa agccctggcc gaggccccca gtccctccgg ttatgaacag ccggcccaga
     3361 aagtcttccg ggagtatatc cagggatgtg ccgatgaagt gcggaccgat gtcctgggaa
     3421 acaccttcgg cttcatacga gggtcgaaga ccaaaccggt cgtgatgctg agcggtcatt
     3481 gcgacgaaat cgggtttatg gtcacgtaca tcgatgagaa tggatttgct tttttttctc
     3541 ccattggtgg ggtggatacc cagctcctgc cggggaagcg ggtctgggtt catacggcca
     3601 aggggtctcg ggtaggggtc atgggaagga aggcgattca tctcatcgac cccaaggacc
     3661 gggagaaggt ggtccagatt cacaacctct tcattgactt cggggccaaa gataagaaag
     3721 aggccgagtc tctggtgaaa atcggagacc ccatcacctt tgctgtaggc ctggagagac
     3781 tgcaaggaga ccgggtagtc tcccgggggt tcgacgacaa gatgggaagc tttgtagtgg
     3841 gcgaggtgct ccgccggatt tgggagagga aagagcgccc ccgctgttcc gtctatggag
     3901 tttccacggt gcaggaagag atcggcttgc ggggagctac aaccagtgcc taccaagtcc
     3961 agcccgatgt aggaatcgcc gtcgaggtgg gccacgccag tgattatccg gaggtcgaca
     4021 agaagagggt cggagagtac aagctaggag ccggcccaat catcgcccga ggggccaata
     4081 tcaaccccgc ggtcttcgcc cttttgcaga aggcggccaa gagaggcaag attccgattc
     4141 agattctcgg ggctccccgg gccacgggaa cggatgccaa cgtcattcag ctctcccgca
     4201 agggagtagc cactggcctg gtaagcgttc ccctccggta tatgcacact ccagcggaag
     4261 tcctttccct gaaagacctg gaggccactt gcgacctgct ggtagaattc atccatctgc
     4321 tcagggagga tatgagcttc attccgggat gaaaacgttc cgggttccca gttgccggtt
     4381 caagccaaaa aaccgaagat tttgaccgac aacggagaac gggcaacggg aattcagtct
     4441 ttctccccga tgccgtactt cttcatcttg tagcgcagga ctcgctcgct gattcccaat
     4501 tcctccgccg ccttggtttg aacgctgtgg ctgctttcca aggcctgggt gatgcgtaac
     4561 cgttcgattt cttccagggt ttccggaagg cttctccttt tcccggcctc cttcatcaaa
     4621 tcctccacct tcccctcccg aaggtttagg ggtaggtctt cggtcatgat cgtatccccg
     4681 cgggaaagga ccacggctct ctcgacgatg ttctccagtt cccggatatt ccccgggtag
     4741 ttgtaattca ggagaaggtc tcgggcttcc ttggataagg aaagcatcga cttctgattt
     4801 tcccgggtgt atttcttaag gaaatgctcg atgagtggag gaaggtcttc ttttcgttcc
     4861 cgcagaggcg gcaggaagat ggtcaccaca ttcagacggt aatagaggtc ttcccggaag
     4921 gtccccttct ggatctcttc ctcaaggtca cggttcgtgg ctgcgacgac ccgtacgtct
     4981 actttgagcg tctggctgct tcccacccgt tcaaattcct tttcctgcaa gacccgcagg
     5041 agcttgctct gcagggagga agataggtct ccgatctcat ccaaaaaaat cgttccctgg
     5101 tccgcctgtt cgaaccgtcc gatcctgcgg cttacggctc cggtgaaggc tcccttttcg
     5161 tggccgaaga gctcgctctc caagagattg tcagggaggg ccgcgcagtt tacctttacc
     5221 aatggcctgt cggccctggg actggagtaa tgaatggccc gggcgatgag ctcttttccc
     5281 gtcccgctct ctcccagaat caagaccgtt gcggcgctgg gagcggcacg tttgaccagg
     5341 ttcagggcct cttccatgcc ccggctggct gaaatgatga aatcgacctt gaatttctcc
     5401 cgcagctgat ccctgaggtc tcggttttcc cgtctcaggc ccaattccct ctccgcccgc
     5461 tggatcagga gcaggagttc atccaggtcg atgggtttgg ttaggtaatc gtaggccccc
     5521 tccttcatgg cggccacggc tgttcccacg gttccaaagg cggtgaggat cacgaccacc
     5581 acttccgggt ttaaccgttt cagttctttg agcagctgaa tcccgtccat tcccggcatc
     5641 cggtaatcgc tcaaaaccag gtcgaaggaa ctccctttgt aactgtcgag cgcttgggcc
     5701 ccgctgccgg cagtctccac tccgtagccc tgtttgccca gaaagccctc cagcatttcc
     5761 cgctggactt gctcatcatc gacgatcaag acctggaatt tgggagtccc cttcatcttt
     5821 cctccccggg aaccggcagg gtcactcgaa acgcagttcc ctttcccgcg acgctgtcca
     5881 cctcgatggc ccccccatgg tctcggatga tcttgtaggc caggggcaaa cccaatccca
     5941 tccccttttc tttcgtggta aagtagtaat cgaagatcct gcccagattc tcttccgcaa
     6001 tccctgcccc cgtatcggat acccggacga tgacttccct tgagggtgaa ttgtatcggg
     6061 caccaatttc caggtttcct ccctggggca tggcctgaag ggcattattc accaaattcc
     6121 ataaggcttc atgaatcctc tggcggtcga gatttaaccg gggaaattcc ccggccagac
     6181 tctcgatcag ttgaacgttt tggcgctcgg cggtctcccg ggcgagcagc agaaggtccc
     6241 tcaaaacctc ccggatttga acggattgaa ggtccaggcg ggctggacgg gcgaagaaga
     6301 ggaattgctc gataatctgg tttactcgtt tcacttcacc ccggaggaca tcggtgaaac
     6361 ggaggtattc cttctgagct tccggaacag taggggcaaa ctcccgctgc agacgttgga
     6421 tggccatgcc gatggcattc aggggattgc gaatttcatg ggccacgccg gcggccaaat
     6481 tggccaggga ggacagcctc tcggactggc ggaccttctc ctccatctcc cgcacccggg
     6541 ccaagtggcg gttttccatg cgggagatga ggacgatggc cgctcccccg aaaaccagaa
     6601 ggaagagact gaagagaagg aggttcttct ggtcgttctt cagaatctcg tccacccgcg
     6661 cggtggacag gccgacccgg aggatccccc ggctgtcggc atccagaacc acccgcttgg
     6721 ccaactccaa tattttctct tcccccaatt cccgggccat ggcttcccgg gtttccccca
     6781 ccggtaggtg ccgcaaaaaa ccgctctctt ccttccgacc gatccgctcc ggagcagtgt
     6841 ccgcccagac ctccaagtcc tccccctgga agatgatata acggatttct cccctgcctt
     6901 tccattcttc gattaacccc tggagaagcg ccctgcggcg aaggtttcgg atagaccgtt
     6961 catccgcacg gacggccagg atcccctctc cggcccttcg gacgatcgcc accgagaggt
     7021 gatccatctg gccggtttcc tttttttctg accgctggat ggcataggcg gttctccctt
     7081 ctaggagggg gcggatgaag gggtctcctc cccggagccc ttctcccgac tggggatagg
     7141 tcaccgtttg ccggggagag ataaaggtta tcccggatag ctgccaggtt ttggccatgc
     7201 ccgccaaatc cttttcctcc ggggcgcggg agccgatctg ccggtccatc tgaaaagcca
     7261 ggtccagaag gagatcgaca acggaatcgt ccagggccaa cggattcacg ggagaaggga
     7321 tcaggagcgc cgaagcctcg gggaatgttt ccagggcagt taccgaagcg aaggaagtct
     7381 gggcattctt ttccaggccg gcgatgaggg cggctccctc gtcatacaga aggcggtgga
     7441 gattgtcttt tagccgcttg gtctcgtaga ttccgttgat aagcaggaca agggccagga
     7501 gaccccatat gatcagaaga aacagggggc gaaagaccca gcgtttcccg cgctcaggcc
     7561 ctttggggag gaaggccata accggcgtgc cgctccatca gtattctttt tccatacccc
     7621 ggccgcgcca cggcatcatg ggaggcccgt aaatccctcc ccaatggggc ggaaggagct
     7681 tgatctgctc cggggtgagg actttccgca tttcgataag caacagcagc ccccgttcct
     7741 ggattttggc ttccaggtcg aagatctctt tttgcttggc gagaatcgtg ttggagtcgg
     7801 attggggatt gcgcagcaag tcccgaatgt cgaaccgttt gatcaccagt tcattcctcc
     7861 agaccagggt gtccctcagg taggtttccc gcaattcttg gagtttagaa gattggtccg
     7921 cggtgagatt cagctggctg gcccaatcca tcatgggagc atgcatgggc cccatcacgc
     7981 ccctgtcccc gcgatcttcc cagcctttcc tccacggggg tccctgggcc ggggcggcct
     8041 g
//
LOCUS       DASPRR010000636         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1443350, whole genome shotgun sequence.
ACCESSION   DASPRR010000636 DASPRR010000000
VERSION     DASPRR010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443350"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VEL68_23295"
     CDS             complement(<1..600)
                     /locus_tag="VEL68_23295"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXZ38974.1"
                     /translation="MESQDRKEEKLLAELETMYQRVAESEKSEGDSMQKDALQSYYES
                     LQISPDAPLEKIKETYERLVAFWQPDQFADTSPLRQEAERKLAEITHAYEKILALRQR
                     ESRRPFAEPPQEISEAPDRSTPDEETGRHFPWGQILLGGAAFVAVLLAAFFWPTLYHY
                     ATIPSGDRTYQIRTNRLTGSVTYFDGANWINLPVPVGQPS"
     gene            complement(775..1413)
                     /locus_tag="VEL68_23300"
     CDS             complement(775..1413)
                     /locus_tag="VEL68_23300"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXZ38975.1"
                     /translation="MRPLGELTHYEILDLPAEATAFEVRKAYKRALEIYSEDSLVIYS
                     LFSAEERKEIQVRLNEAFGTLINERARYEYDQRLIQQGVIKEGPQYRSDGEKGASVGP
                     EGSADPASQPEGEKPQAVENPVVKEILSQEALTGMDLKRLRMELGVSLDQISEWTKIR
                     PGMLRCIEEDRFYELPSRLHLRSFLKAYVQYFHLNPESVVGRYMKRIEGESS"
     gene            complement(1427..>2225)
                     /locus_tag="VEL68_23305"
     CDS             complement(1427..>2225)
                     /locus_tag="VEL68_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38976.1"
                     /translation="QSKSLSDFINKKVRTLAETVIETRIPNLFLISGAGNNLDTANMA
                     YEQKMKIFRAISNLSYDFTLLDLGAGTSFNTIDFFLISDSGIFVVTPEPTAIENMYRL
                     IRAVYARKIRQLLKTYHFRTLVEETEARKNGAMVNNPEYLLSVIRELEPEKGKIVEQA
                     LRAFQFKLVLNQLRKRDNPRLGALICRIIEKHLGLSLQFIGNISYDDLVHESVCQKVP
                     FLEKYLHTQTAADLEKFSKHILGAGTRGEGSGPAGGALETAAVGREQ"
BASE COUNT          468 a          591 c          584 g          582 t
ORIGIN      
        1 agagggttga cccaccggga ccggaagatt gatccaattt gccccgtcga aataagtcac
       61 gctgcccgta aggcgatttg tcctgatttg ataggtccta tctccggatg ggatcgtggc
      121 ataatgatag agggtcggcc agaaaaacgc tgccaaaagt acggcaacga aagcagcccc
      181 ccccagaaga atttggcccc aggggaaatg acgacccgtt tcttcgtctg gggtcgaacg
      241 atctggcgcc tcggaaattt cttggggggg ttccgcgaat ggacgtctgc tttccctttg
      301 tcgcaaggca agaattttct cataggcatg agttatttct gctaacttcc tttccgcctc
      361 ctggcggagg ggagaggtat cggcaaactg atcgggctgc cagaaagcga ccaaccgctc
      421 ataggtttcc ttgatttttt ccaggggagc atcgggggat atctggagag actcgtaata
      481 gctttgcagg gcatctttct gcatgctgtc cccttcagac ttttcggatt cagcgacccg
      541 ctggtacatg gtttccaatt cagcgagaag tttctcttct ttgcggtcct gactttccat
      601 gttacggatc cttacgggtg aagggttccc cccgtcttgt ttccggcctg ggttcaaagt
      661 ctcgcctgga tctgtttgcc cgggacaacc gtttaatagc cctggaacca gacccttctg
      721 tgaaaaaccc cgctgtgcgc accggagacc tcacagcggg ataaaggtcc tttttcagga
      781 actttccccc tcaatcctct tcatataacg gcctacaacg gattctggat tcaggtgaaa
      841 atactgcacg taggctttca ggaaactcct taaatggagg cgagaaggca actcgtagaa
      901 ccgatcctcc tcgatacacc ggagcatgcc gggccgaatc tttgtccact cggaaatctg
      961 gtccagggaa acccccaatt ccatcctcaa ccttttcagg tccattcccg tcagggcctc
     1021 ctgggagagg atttctttga ccaccggatt ttccactgcc tggggttttt ccccttccgg
     1081 ttgggaggcc gggtctgcgg agccttccgg tcccaccgag gccccctttt ccccgtcgct
     1141 ccggtattgg ggaccctctt tgataacccc ctgttgaatc agcctttgat catattcgta
     1201 cctggccctc tcgttgatca aggtgccgaa cgcttcgttg agcctaacct ggatttcttt
     1261 tctttcttcg gcggagaaaa gggaataaat caccagcgag tcttcactat agatttccaa
     1321 cgctctttta taggccttcc ggacctcgaa ggctgtggcc tccgcaggaa ggtccaggat
     1381 ttcgtaatgg gtcaattcgc ccaagggcct catcggattc tattcctcat tgttctcgcc
     1441 cgactgcggc cgtctccagg gcgccgcctg cgggaccgga tccctccccc ctggttcccg
     1501 cccccaggat atgtttggaa aatttttcaa ggtccgccgc ggtttgtgtg tggaggtatt
     1561 tctccagaaa aggaactttc tgacagaccg attcatggac caggtcgtca taggagatgt
     1621 tgccgatgaa ttgcagggag aggccgaggt gcttttcaat aatgcggcag attaaagccc
     1681 ccaatctggg gttatcccgt ttgcgaagct ggttcagaac cagcttgaat tgaaaagccc
     1741 gcagagcctg ttccacgatt ttacccttct ccggctccag ttctcggatg acggaaagaa
     1801 ggtattccgg gttattgacc atcgccccat ttttccttgc ttccgtctct tccaccaggg
     1861 ttcggaaatg ataggttttg agaagctgcc ggatcttgcg ggcgtacacg gctcgtatta
     1921 ggcggtacat attttcaatg gccgtaggct cgggggtaac aacgaagata cccgaatcgg
     1981 agatcaagaa gaaatcaatg gtgttaaagg atgtaccggc tcccaggtcc aagagggtga
     2041 agtcgtaaga gaggttggag atggcccgaa agatcttcat tttttgctcg taggccatat
     2101 tggctgtgtc caggttgttc ccggccccgc tgatcaggaa gaggttggga atcctggttt
     2161 cgatgaccgt ttcggccaga gttctgactt ttttatttat aaaatccgaa aggcttttgg
     2221 actga
//
LOCUS       DASPRR010000637         9160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1443909, whole genome shotgun sequence.
ACCESSION   DASPRR010000637 DASPRR010000000
VERSION     DASPRR010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443909"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VEL68_23310"
     CDS             <1..319
                     /locus_tag="VEL68_23310"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXZ38977.1"
                     /translation="KALNGPIDFMEKRNQTFQERRDVVVQGLREIGLEVESPQATFYV
                     WSTIPGGYTSRDFCYRVLEEANVWMIPGSMYGKCGEGYLRIALTHPVERLAEAMNRLK
                     KFK"
     gene            355..753
                     /locus_tag="VEL68_23315"
     CDS             355..753
                     /locus_tag="VEL68_23315"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXZ38978.1"
                     /translation="MPKVEVRHLQGTRLMGVAREHALIFDKTIEGGGTGLGINVSELF
                     LFSLGACITFNLLQYTATHQMKVQGIRVGLSDEQQSSPNRISRVKAQVYISGEVSEDE
                     LLRLLRSARGCKIHNTLKAVPEIEVSMHRE"
     gene            769..1752
                     /locus_tag="VEL68_23320"
     CDS             769..1752
                     /locus_tag="VEL68_23320"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ38979.1"
                     /translation="MFSSKKKFAGLMVLVSFLFFCPWTAKAADADYPSKPVTLVVGFP
                     AGGAVDLTARALVNGAKKHFPQPIIVENKSGAGGMVGLAQVGVKPPDGYTVGVIGSQA
                     AAASWHMGTTNYNQLDDFTHIMRYAGLMFGIVVRQDSPFNTLLDVITYAKANPGKVSF
                     GTSGVGSTGHLAMATLAQEAGDLQLTHIPYKGGGESLPALLGGHVDVLSDASSWTSLV
                     DAGKFRVLAVYSSERSRMYPSVPTVKESGYAVVYSNPLELFGPKGLPGPIVSKLHDAF
                     RKGMDDPGFDAVLKKMGMPMLYLAGEELLKSNRENFERMKKVVEKLELQKK"
     gene            2016..2342
                     /locus_tag="VEL68_23325"
     CDS             2016..2342
                     /locus_tag="VEL68_23325"
                     /inference="COORDINATES: protein motif:HMM:NF024485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXZ38980.1"
                     /translation="MSEKVIQVKVFRFDPSADKEPRYQTYQVPLEKGMSAMTALDYIY
                     QNLDGTIAYYDHAACDLGICARCTGIINGKPGLFCQTVIQGDVTLEPTSKTKILKDLV
                     VKRGGE"
     gene            2458..2973
                     /locus_tag="VEL68_23330"
     CDS             2458..2973
                     /locus_tag="VEL68_23330"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HXZ38981.1"
                     /translation="MPDKKQMITKEEAFQQVKSMITRAALIHWAFSKTLIGELGEKKG
                     KALAKKAIELYGKEVGKRVKERTLAKSLPLSRENFQDDLPDLGWAEREKVEVEGEKRS
                     RVYTCHLARVWQELGVPELGRIYCYVDQAKYEAYNPELQCVHVKNVLDGDAYCELAVR
                     TKKKQGGLHNP"
     gene            3097..4872
                     /locus_tag="VEL68_23335"
     CDS             3097..4872
                     /locus_tag="VEL68_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXZ38982.1"
                     /translation="MSERTIETDVLIIGGGGAGFRAAIGAREAGVRALLISKGPLARC
                     GATPMAGADFTLDGLSLSQMGFPGEPKDTKEKFFNDIATQGFYLNNQKLLEQYIHAAP
                     ARLKELLDWGMKVDSSEQRAIFTSGLGLVDTVYRRAKECGVDFLEDVMVLDLVTHGGK
                     VVGALGLDISTGDFIQFRVKAAVISTGGWHKAFWPNAGMRDLSGEGIAMACRAGAEIG
                     NMEFITFCCNILLWPPLWRGSLATYILSLRFDAELTNSVGEVFLKKYDPFVVKWGTYM
                     EWNKGFVSFASMREIRDGKGSPHGGVYFGLGSTPYATFEKIALGRFPDWKYKALDLTE
                     MPKIFKEGKLMEVGPVVEYFDGGIVVNEKFETAVEGLYAAGECALGPFGSNRVCSAIT
                     EMLVHGADAAHNAGEYAKKAGRADLPVQALKALGEKAEQALVRMDGLRPAPIRRQVQE
                     MAHKNLSPIRTQKELSAFLSFLDKTIQDDLPHLATASKSRVYNKEWVDAIELENILHL
                     LKCATISALHRTESRGVHFREDFPYTDNDAWLKENIIRFDKGVLEVYRRPVTTTSMTL
                     PKGKVPYLDMLKKMMESRSDVGGHH"
     gene            4945..6066
                     /locus_tag="VEL68_23340"
     CDS             4945..6066
                     /locus_tag="VEL68_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXZ38983.1"
                     /translation="MERRFVAVLFVLGLTIGSFPTLTTAHGPSAPPHQSYSMGDFKLE
                     SGEVINDFAISYVTHGTLNEKKSNAILMVTAISGNHHRLDFLIGPGKALDTTKYFVVA
                     TDAIGNGLTTSPSNSKAQPRMKFPRFTIRDMVQSQYRLMTERLGINHVVAVVGPSMGG
                     MQALQWGVSYPGFMDSLVALVPLAKTPAWTVLILETTRKALMVDPAWNNGNYTTLSEG
                     NLRLWRELQVFVSSRNPTQYRSLFPNGLDLLPWLKEQEEPLLKNFDPSDYIYQTWAYE
                     THDISTTPGMNGNMFKALQAIKAKTLIMTGVNDLLNPEWEPLEAARYIRDVHCVTINP
                     FSVTGHFAGSGLIPADVDFLNREVGSFLDVVTDRGKKLQ"
     gene            complement(6438..7964)
                     /locus_tag="VEL68_23345"
     CDS             complement(6438..7964)
                     /locus_tag="VEL68_23345"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXZ38984.1"
                     /translation="MYKEYYRFSEEPFGLNPDPKFLYLAMSHFKALNSMISSIKERKG
                     LITITGEVGVGKTILIHAFLKGLSKNIKTAFIFNPRFDFKSLLKNILSDLELPIDKNE
                     ESVPFLLEKFKEYLQSRASRDEMVVIIIDEAQGLEDEVLREIERLSRLGDSRERVLQV
                     LLVGQPELEAKLNSEKFRFFKEKVAVHSQISPLTREECRGYIKHRLNLVGRDASEVFT
                     SEALKRVWQFAKGIPRVMNLICDRALLIGYSNTSPIIDSKIVDQAIKDYGYLQVTKPA
                     FPPSKFPRRRSYAIIGLLLLLGMSLAGYFILSRDFAPPDMQFKGKILPSEESSAAQAE
                     VKLIEVKKDLEAEKPPEPKPKPEPKPEEEFFAVREGWVLSAIAQKYYPVLNLSLLDFF
                     LEANPHITNLDSIFRGQTIKIPILTEESLLQQVSKNTYQVYLGTFRTAQEVQPYRNEP
                     ALLKKTIKVFKRQVSPRETWYRVTVGDFRGKEEALKAIHALKGKKLLPLLDCLPKKTT
                     "
     gene            complement(8154..8843)
                     /locus_tag="VEL68_23350"
     CDS             complement(8154..8843)
                     /locus_tag="VEL68_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXZ38985.1"
                     /translation="MKLIDQIARIPLFQGLDRKHYDQLAMIVTDQVFQKGESIFDEED
                     EGTGFYVVISGRVKVFKLSPEGKEQILHIFGPGEPFGEVAVFTGKRFPASAEALEETR
                     AFFFARKEFMDLIQRDPSLALNMLAVLSQRLRRFSGLIESLSLKEVPGRLAAYFLYLQ
                     EGKKGGADLILEISKNQLASLLGTIPETLSRILARMNREGFIDSIGPRQVRILNGKGL
                     EELASGERRLG"
     gene            8996..>9160
                     /locus_tag="VEL68_23355"
     CDS             8996..>9160
                     /locus_tag="VEL68_23355"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HXZ38986.1"
                     /translation="MKWTKEAEEAVCQVPFFVRKRVLKRVEEEAARDGAGQVTLGHVK
                     TCQQRFLNRME"
BASE COUNT         2125 a         2474 c         2526 g         2035 t
ORIGIN      
        1 gaaagctttg aacggcccta ttgacttcat ggagaaaagg aatcagactt ttcaggaacg
       61 acgggatgtg gttgtgcagg gattgcggga aattggcctg gaggtggaaa gtccccaagc
      121 aaccttttat gtctggtcca ctatccccgg gggatatacc tctagagatt tctgctatcg
      181 ggtcctggaa gaagccaacg tttggatgat cccgggttct atgtacggaa aatgtggcga
      241 gggctacttg cgcatcgccc tgacccatcc cgtggagagg ctggccgagg ccatgaaccg
      301 tttgaagaaa tttaaatagg gaaactccgg taggcgggga tttaaggaaa cggtatgcct
      361 aaagtggaag tgcgccacct gcagggcacc cggttgatgg gggtggcgcg cgaacatgcc
      421 ctgatttttg acaagaccat cgaagggggt ggaaccggcc tggggatcaa tgtcagcgag
      481 cttttcctct tttctttggg agcctgtatt acctttaatc tcctgcagta taccgctact
      541 catcagatga aagtccaggg aatccgggtg ggcctttccg acgaacagca atcttccccg
      601 aatcgaatct ccagagtaaa ggcgcaggtc tatatctcgg gggaggtgag cgaagatgag
      661 cttctccgcc tgctgcgatc ggccagaggg tgcaagattc acaataccct gaaggctgtc
      721 ccggagatcg aagtgtccat gcatcgggaa taaatgaagg gagggttcat gttttcatcc
      781 aaaaagaaat tcgcgggcct aatggtcctt gtcagcttcc tttttttctg tccgtggacg
      841 gccaaagccg cggacgccga ttatccctcc aaacccgtaa ccctggttgt cggctttcca
      901 gccggggggg ccgtcgacct tacagcccgg gccctggtaa acggggccaa gaaacacttc
      961 ccccagccga tcatcgtgga aaacaagagc ggcgccggcg gaatggtcgg gctcgcccag
     1021 gtgggcgtga aaccgcccga cgggtacacc gtcggggtca tcggcagcca ggctgccgca
     1081 gcttcctggc acatgggcac aaccaactac aaccagctgg atgactttac ccacattatg
     1141 cgctacgcag ggcttatgtt cgggatcgtg gttcgccagg attcgccgtt caacaccctg
     1201 ctggatgtca taacctacgc caaggcaaac ccggggaagg tttccttcgg aacctcgggg
     1261 gttggttcaa ctgggcatct ggccatggca accctggccc aggaagccgg ggacctccag
     1321 ttgacccata tcccttacaa gggcggggga gagtctcttc ccgccttgct tggagggcat
     1381 gtggatgtgc tgtccgatgc ctcgtcctgg acttccttgg tcgatgccgg caaatttcgg
     1441 gtcctggcgg tctactcctc ggaacgcagc aggatgtacc cttcggtgcc cacagtcaaa
     1501 gagtctggtt atgccgttgt ctacagcaac cccctggagc tcttcggccc caagggattg
     1561 cccggtccca tcgtctccaa gctgcatgac gctttccgta agggtatgga cgacccggga
     1621 ttcgatgccg ttctcaagaa aatgggcatg cctatgctct atctcgcggg ggaagaactg
     1681 ctcaaatcaa accgcgagaa ttttgaaaga atgaaaaaag tggtagaaaa gctggaactg
     1741 cagaagaaat aggtcgctca gccgcaagcg attcggccct aatcaggggg gtttgatcat
     1801 gggtgctgat ttattcccag gcaagcatct cggccaggct tcaacccata cccgaggctc
     1861 gcgcgtacca tgaccatcat gaaaggggag gacctctgcg atttccgtta ctcgctaaaa
     1921 ggatgaagac acccagagcg tagcgttcaa tcccaatatt ggccattggc tcttacacta
     1981 tgctctatgc cccttgcctt tttgaggaga aggttatgtc ggagaaggtg attcaggtga
     2041 aggtatttcg tttcgatcct tcggcagaca aagaaccccg ctaccaaacc taccaggtcc
     2101 ccctggaaaa gggcatgagc gccatgaccg ccctggatta catctaccag aacctggacg
     2161 ggaccatcgc ctattatgat catgcggctt gcgacctggg catctgtgcc cggtgcacgg
     2221 ggatcataaa cgggaagccc ggcctcttct gccagacagt catccagggg gatgtgaccc
     2281 tggagcccac ctcaaaaacc aagatcctca aagacctggt cgtaaaaaga gggggagagt
     2341 gaagattctg atcctgccgg aggatcggtt aagatttggc ggaaaagcgt gtaggggcgg
     2401 ttcgcgaacc gcccctgcat ctggacagaa actgcaattg gcaagaaggg gaaccatatg
     2461 ccagataaga aacaaatgat aaccaaagaa gaggccttcc agcaggtcaa gtctatgatc
     2521 acccgggcgg ccttgattca ctgggccttt tccaaaactc tgatcggcga gttaggcgag
     2581 aaaaagggta aggcgctagc caaaaaggcg atcgaactct atgggaagga agtcggaaag
     2641 cgggtgaagg aacggacttt ggcgaaaagc cttcccctga gccgggagaa ttttcaggat
     2701 gacctccctg atttgggttg ggcagagcgg gaaaaggtgg aagtggaagg agagaaacgt
     2761 tcccgagttt acacctgcca cctggccagg gtgtggcagg agcttggcgt tcccgaactg
     2821 ggccggatct attgttatgt tgaccaggct aaatacgaag cctataatcc ggaacttcag
     2881 tgcgtccatg tgaaaaacgt gctggacggg gatgcatact gcgaactggc tgtacggacg
     2941 aaaaagaagc agggggggtt acacaatccc tgacgtcgaa cgttgaactt cgaacaccca
     3001 acgtcgaact tgaacgttgg aagatggatg tttgatgttg aacctcagtg cttactatca
     3061 aatcttttca actcttcgct cttttgggag attttcgtgt ctgaacggac catcgaaaca
     3121 gatgtcttaa ttatcggagg cggaggggct ggcttccggg ccgccatcgg cgctagagag
     3181 gccggcgtcc gcgcgcttct catcagcaag ggtcccctgg cccggtgcgg cgctactccc
     3241 atggccggcg ccgattttac cctggacggg ctgagcctga gccagatggg tttccccggc
     3301 gaaccgaagg atacgaagga gaagttcttc aacgacatcg ctacccaagg cttttacttg
     3361 aacaaccaga agctcctgga gcaatacatc cacgcggctc cggcgaggtt gaaagagctt
     3421 ctcgactggg ggatgaaggt cgacagctcg gaacagcggg cgatctttac ctccggctta
     3481 ggactggtgg ataccgtcta tcgccgggcg aaggaatgcg gcgtcgattt cctggaagat
     3541 gtgatggtcc tcgacctcgt gactcatggg gggaaagttg tcggggctct gggtttggat
     3601 atatccacgg gcgatttcat ccaattccgc gttaaggccg cggtgatctc cacaggagga
     3661 tggcacaagg cgttttggcc caacgcgggg atgcgggacc tgtctgggga gggaatcgcc
     3721 atggcctgcc gggcaggtgc cgagatcggc aacatggaat tcatcacctt ctgttgcaac
     3781 atccttctct ggccccccct ctggagggga tccctggcga cttacatctt gagcctccgg
     3841 ttcgacgccg agctgaccaa cagcgtcgga gaggtcttct taaaaaaata cgatcccttc
     3901 gtggtcaaat ggggcactta catggagtgg aacaagggat tcgtatcctt cgcctccatg
     3961 agggaaatac gggacggaaa aggctctcct cacggggggg tctatttcgg tctgggttct
     4021 accccttatg cgacctttga aaaaatcgct ttgggccgct ttcccgactg gaaatacaaa
     4081 gctctggatt tgaccgagat gcccaaaata ttcaaagagg ggaagctcat ggaagtgggt
     4141 ccggttgtgg aatacttcga cggcggcatc gtggtcaacg aaaaatttga gacggctgta
     4201 gaggggctct acgccgccgg ggagtgcgcc ttgggcccct tcggctccaa ccgggtctgc
     4261 tcggccatca ccgagatgct cgtccacggg gcggatgccg cccacaacgc cggagagtac
     4321 gcaaagaaag ccgggagggc cgatcttccc gtccaggctt taaaagccct gggagaaaag
     4381 gcagaacagg ccttggtgcg gatggacggc ctcaggccgg ccccgattcg caggcaggtc
     4441 caggaaatgg ctcataagaa tttgagcccg atccggaccc agaaggagtt atccgctttc
     4501 ctttccttcc tggacaaaac catccaggac gatcttcccc acctggccac cgcctccaaa
     4561 agcagggttt acaataaaga atgggtagac gccatcgagc tcgagaatat actgcacctg
     4621 ctgaaatgcg ccactattag cgctctgcac cgaacggaaa gccggggcgt tcatttccgg
     4681 gaggattttc cttacacgga taatgatgcc tggctgaaag aaaatatcat ccgcttcgac
     4741 aagggcgtcc tggaggttta ccggaggccg gtcaccacca cctccatgac cctccccaaa
     4801 gggaaagttc cctacctgga tatgcttaaa aagatgatgg agagccggtc ggatgtggga
     4861 gggcaccact gaaaggcagg aacgaggtcc gagaataaag ataaaggaaa agagtagcga
     4921 aacgagaaaa ggaggaaatt gcctatggaa agaagattcg ttgcagtcct tttcgtactt
     4981 ggcctgacga ttgggtcttt cccaacactg acgaccgccc atggtccttc ggctcctccc
     5041 caccagtcat acagcatggg ggatttcaag ctggagagcg gggaggtcat caacgatttc
     5101 gccatttcct acgtcaccca cggaaccctg aatgagaaaa agtcgaatgc catcctcatg
     5161 gtgacggcca tcagcggcaa ccaccaccgc ctggactttc tgatcggccc ggggaaagct
     5221 ctggacacga cgaaatactt tgtcgtggct acggatgcca tcggcaacgg cctcaccact
     5281 tccccctcga acagcaaagc ccagccgagg atgaagttcc cccgtttcac catccgcgac
     5341 atggttcagt cgcagtaccg cctcatgacc gaacgcctcg gtatcaacca cgtggtggcc
     5401 gtagtggggc cctctatggg aggcatgcag gccctccagt ggggagtcag ctatccaggt
     5461 ttcatggaca gtctcgtagc gctggtcccc ctggccaaga cccctgcttg gaccgttctc
     5521 atcctcgaaa ccacccgcaa agccctgatg gtggacccgg catggaataa cggcaactac
     5581 accactctat ccgaggggaa cctccgcctg tggcgcgaac tccaggtctt tgtgagctcg
     5641 cgcaacccga cccagtatcg ctccctcttc cccaacggac tcgacctgct cccctggctc
     5701 aaggagcaag aagagcccct gctcaagaat tttgacccaa gcgattacat ctaccagacc
     5761 tgggcttacg agacccacga tatcagcacc accccgggca tgaacggaaa catgtttaaa
     5821 gccctccagg ccatcaaggc caagaccttg atcatgaccg gggtaaatga ccttctgaac
     5881 ccggagtggg aaccattgga ggccgcccgc tacatcaggg acgtgcactg cgtaaccatc
     5941 aaccccttct ccgtgaccgg ccattttgcc ggcagcggat taatcccggc cgacgtggat
     6001 tttctcaatc gggaggtcgg gagcttcctg gatgtggtaa cggaccgtgg gaaaaagctc
     6061 cagtagtcaa ggaacacctg ggaggtcccc ggagggcctc ccagacgagt tcctccgttg
     6121 atcaccgtca gcctgaagat cccctgagag acctggccaa caccatctat ccgggatcaa
     6181 gtgaaatccg ccaggtccca agccggaaaa acgaaattca tcctgttgcg gatgctccct
     6241 tccaacctac agcttccccc cccttgatcc aggcagccag ggacgcttcc cggagtcgat
     6301 gaccttttca gcttattatt gcggagccgg ggtatcaacg aatagggggc aaggatgaaa
     6361 gggagctttc tcttttccag tctccttcgg gagaatcgcg gcctatagtc tgggttatgc
     6421 tttttggtct tgcccagcta ggtggttttc ttcggaaggc aatcgagaag gggtagcagc
     6481 ttctttcctt tcagggcgtg aatggctttc agggcctcct cttttccccg gaaatccccc
     6541 accgtgaccc tgtaccaggt ttcccggggc gaaacctgcc gcttgaagac tttaatagtc
     6601 tttttgagca aagccggctc attcctgtac ggctgcacct cctgggccgt ccggaaggtc
     6661 cccaaataaa cctgatacgt atttttcgaa acctgctgga ggagggattc ttcggtaagg
     6721 atcgggattt tgatagtctg cccgcggaag atcgaatcca gattggtgat gtggggattg
     6781 gcttccagaa agaaatccag gaggctgagg ttgaggactg gatagtactt ttgggcgatc
     6841 gccgaaagaa cccacccctc tctcacggcg aagaattctt cttcaggttt cggctcgggt
     6901 ttaggttttg gctcgggcgg tttttcagcc tccaagtcct ttttcacttc gatcaatttg
     6961 acttccgcct gcgctgcgct actttcttcc gaaggaagga tcttgccctt gaattgcata
     7021 tccggcggag cgaaatcccg gctaagaatg aaataccctg ccagactcat tcccagaagg
     7081 aggaggagtc caatgattgc ataactcctt cggcgaggga atttcgaggg ggggaatgca
     7141 ggcttggtga cttgcagata tccataatct ttaatcgcct ggtcgacgat ttttgaatcg
     7201 ataatggggc tggtgttgct atacccaatc aaaagagctc ggtcacagat tagattcatc
     7261 acccggggga tccccttggc aaactgccag acacgtttta gggcttcgga agtgaacacc
     7321 tcggaggcat ctcttcctac cagattcaat cggtgtttga tgtatcccct gcactcctcc
     7381 cgggttaggg ggctgatctg cgaatgaacc gcgactttct ctttgaagaa gcgaaacttc
     7441 tcggaattca gctttgcctc cagctccggc tgccccacca acaggacttg gagcaccctt
     7501 tcgcgcgaat cacccaggcg cgaaagtcgc tcgatctctc tcaggacctc atcttccaat
     7561 ccctgagctt catcgataat aatgaccacc atttcgtccc ggctggccct tgattgaaga
     7621 tattccttaa acttctccag gagaaaaggg acgctctctt cattcttgtc gatgggcagt
     7681 tccaggtccg aaagaatatt cttgagcaga cttttgaagt caaacctggg gttgaaaatg
     7741 aaggcggtct tgatattttt gctcaagccc ttcaggaagg cgtggatgag aatggtcttt
     7801 cccactccca cttctcccgt tatcgtgatc aagcctttcc tctctttgat cgaggaaatc
     7861 atggagttga gagccttaaa gtggctcatg gccagataaa ggaatttggg atcgggattg
     7921 agcccgaaag gttcctcgga gaaccggtaa tactctttat acattgttca tccgctcgaa
     7981 tacctaggtt gggtcttctc ttcggttgag ctgatcggct tcccaacttt attttgatgc
     8041 taacaaaccc gccaatctag tcaagtggtt ttttcggctt ccaattctta atggcaaaat
     8101 aaatgctttc cttttcatcg gctcacaact taccgcctac aacactccct tctttacccc
     8161 agccgcctct ccccgctggc cagttcctcc agaccttttc cgtttagaat tctaacctgc
     8221 cgaggcccga tcgagtcgat gaagccctct cggttcattc tggccaagat gcgagaaagg
     8281 gtctccggaa ttgttcccag caaactggcc agctggtttt tggagatttc aagaatcagg
     8341 tccgctcccc ctttcttccc ctcctggagg tacaggaaat aggctgccag ccttccgggt
     8401 acctctttca acgaaaggct ctcgatgaga cccgagaaac gccgcaggcg ctgggaaagg
     8461 accgccagca tgttcagagc cagagaaggg tctctttgga tcaaatccat gaattctttc
     8521 cgggcaaaga aaaaggcccg ggtttcctcc agggcttcgg cactggccgg gaaccttttc
     8581 ccggtgaata ccgccacttc tccgaagggc tctcccgggc cgaagatatg gaggatctgt
     8641 tctttgccct ccggtgagag cttgaagacc tttacccgtc cggagatgac cacatagaag
     8701 cctgttcctt catcctcttc atcgaagatg ctttcccctt tctggaaaac ctggtcggtc
     8761 acgatcatgg ccagctggtc atagtgtttc cggtccagtc cctgaaacaa gggaatccga
     8821 gcaatctggt cgatgagttt catggaatgc ctcctcaagg ggaattatct tcatcttttg
     8881 acttatgtca aggcccatga ccgtaaaatt aggttataaa ataaagggta aaggaaaaaa
     8941 aggagccctg gtcccgacgt ctaggacctt gcgccaattc tttggggggt aacccatgaa
     9001 atggaccaag gaagcggagg aggcggtatg ccaagtcccg ttcttcgtcc gtaagcgggt
     9061 gctgaagaga gtggaagagg aggccgcccg cgatggggct gggcaagtca ccctgggaca
     9121 cgtcaagacc tgccagcagc gattccttaa ccggatggaa
//
LOCUS       DASPRR010000638         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1931232, whole genome shotgun sequence.
ACCESSION   DASPRR010000638 DASPRR010000000
VERSION     DASPRR010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931232"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VEL68_23360"
     CDS             <1..282
                     /locus_tag="VEL68_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXZ38987.1"
                     /translation="RVIGREALWMLEMGVASFEDIDKACVYGAGHPMGPFRLMDLTGI
                     DLNYIMSLERFKKTGDRSDLPSPSVIERYLKGQYGEKTGKGWYDYTQKK"
     gene            451..1689
                     /locus_tag="VEL68_23365"
     CDS             451..1689
                     /locus_tag="VEL68_23365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXZ38988.1"
                     /translation="MKKTLFLLAIFTALLGLATASAQDIKIGFMGNLSAPSSLSSKAA
                     AQMAVDEMNAAGGIQGKQIKLIIEDTKGEIPKCVEIYKKLVMVDKVLAVVIAEKVEMG
                     VAGMEIGAELFREYPHIFFSTIGSGDDIWHHVRDNYNKYKFGFQTYYSISTNYLKIWG
                     ELNSQVFKDLVKTKKAALIYEDMEWTKPLRRGLKDVSPRLAEVYKNKGLEVVYETTLS
                     LDQKMFNPIFEEVAKSGAGIMDCVVGYIDEAAFVKQWAQSSARNIPFYIWGGLAGMPQ
                     AWKMTEGKVAGGMVGSSMVKVPITPKTIPFMENLVAKYKVGPIFGSHTTYDTLFGFKK
                     AIEKAGGASNVENLIKQLEQVEEVAVFGTIGWEPKYHYNLPYPKYITPVVQWQKGDMK
                     VIFPAQYKTGEYLPPAEARK"
     gene            1766..2641
                     /locus_tag="VEL68_23370"
     CDS             1766..2641
                     /locus_tag="VEL68_23370"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38989.1"
                     /translation="MIETLHSILIYTFIWGSIYLLISLGFSLICGVLRIFHMGYGATF
                     VLAAYAVWTFMEEANLGLIPSILLMALVQIGFSLVVFYRGVFQRYLDQEEILLTLSIL
                     IFVTLTHLANFLFPVTAGVSIPTTIIDDTLVIGKVRIAYQMLLAALAGILVTALYVLW
                     FLKTRTGLIIRAISQNLPAALLMGAGVNRMYYLAMVLSVIPPTICMMMIAPFWGVDPF
                     MGSPLLMTAMLIAILGGLGNLKGSIVASYLIGFIHASVSFIYISRFMGLAALVVTLAV
                     MIYRRGGIFAGETLW"
     gene            2635..3678
                     /locus_tag="VEL68_23375"
     CDS             2635..3678
                     /locus_tag="VEL68_23375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ38990.1"
                     /translation="MVNLELRRDRVLFRWKDQKYEWVIEPRYWRNPAIGVALLASLPL
                     FVSPFPHILTMITTANLYAAVTIPLGLQITGTARVNFGPQLYLGIGGYAAALMNLHLG
                     ASPAVTLLGTILVCGGIAFLLSPTTWIAKGLYFSLISLILPLTFLDITFIFGDIFRGE
                     VGLVGMSPLLSLRKVNWNYIAYFYLSLGLMLFYLLVCDKIGRSRFGVSMAAINENEDV
                     ARMMGVPVDRYKVFFYVFPSILTGIVGWYIVHTFRTFAGVTYLPLEFMTKILIIVIVG
                     GRAFLYGAIPGAYLIAVVEDSLREFGPINYVLFPLVLIALIVFLPRGEGLWGLYHKRH
                     HRDYYPVLHVRRD"
     gene            3682..4419
                     /locus_tag="VEL68_23380"
     CDS             3682..4419
                     /locus_tag="VEL68_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38991.1"
                     /translation="MLRINRVSKRFGGLYAVNEVSFSLDQGEVIGLIGPNGAGKTTLL
                     NLICGVYKADGGSIEFLGQEISGMPSNKICRLGISRTYQIPQPFSAMTALAGVAVSVI
                     NGKRRPNMCLSDAALEASYYLEFVGLFSKRDALARDLTLYELRMLELARALATTPKLL
                     LIDEVMAGLNPAEASRALEMIWSAQEQFGFTILWIEHVMKIIMSAAQRVVVLQYGRKI
                     AEGPPREISSDPKIIEAYLGSRHAKGH"
     gene            4403..>4986
                     /locus_tag="VEL68_23385"
     CDS             4403..>4986
                     /locus_tag="VEL68_23385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ38992.1"
                     /translation="MLKVIDWNASYGVIPVLHNVSLEVKPGEIVALLGSNGAGKTTLL
                     NSISGIVKGRSGTISFFDKGIGNLPPNKIVETGIAQIPEGRKIFPYLTVKENLLMGAC
                     ILQAWRERQIMLEKVTALFPILKERSGQRASLMSGGEQQMLVIARGLMARPRLLLIDE
                     PSLGLSPIIMGQIYEAIKTFPRDGITVLLSEQNAL"
BASE COUNT         1021 a         1502 c         1393 g         1070 t
ORIGIN      
        1 cgggtgatcg gccgcgaggc cctctggatg ctggagatgg gggtggctag cttcgaggat
       61 atcgacaaag cctgcgtcta cggcgccggg caccccatgg gcccgttccg gctgatggac
      121 ctgaccggga tcgatcttaa ttacatcatg tccctggagc gtttcaaaaa gaccggcgac
      181 cggtccgacc ttccctcccc cagcgttata gaaaggtacc tgaaaggcca gtacggggaa
      241 aagaccggca aaggctggta cgattacact cagaaaaaat aaagccattc ctcttccctg
      301 ctcctccatg gattgcccgg agagactctt ttccttcccc ggaaccaggg ggtttcgacc
      361 ccctgggaaa gggaactttt gtcgttcgtt aaaacgggaa tttcccccgt aggcaattca
      421 gttatcaact tcttcttcag gagggaaatc atgaaaaaga cgttattttt actggctatt
      481 tttaccgcct tactgggcct ggcaacggcc tccgcccagg acattaaaat cggcttcatg
      541 gggaacctgt cagccccctc ttccctgagc tccaaggccg cagcccagat ggctgtggac
      601 gaaatgaacg cggccggcgg aatccaggga aagcagatca agctcatcat cgaagacacg
      661 aagggagaaa tccccaagtg cgtggaaatt tacaagaagc tggtcatggt ggataaagtc
      721 ctcgccgtgg tcattgcgga aaaggtggag atgggcgtgg ccgggatgga aatcggtgcg
      781 gagctattcc gggagtaccc ccacatcttc ttcagcacca tcggttccgg cgacgatatc
      841 tggcaccacg tgcgggataa ctacaacaaa tacaagttcg gctttcagac ttattattcc
      901 atctccacca actacctgaa aatctgggga gagctgaata gtcaggtctt caaggacctg
      961 gtcaaaacca agaaggcggc cctgatctat gaagatatgg aatggaccaa gcccctgcgc
     1021 cgggggctga aagacgtctc cccccgactg gcggaagtgt acaagaataa ggggctggag
     1081 gtggtgtacg aaaccaccct ctccctcgat cagaaaatgt tcaatccgat ctttgaggaa
     1141 gtggccaaat ccggggcggg aatcatggac tgcgtggtag gttacatcga cgaggcggcc
     1201 ttcgtgaaac agtgggccca gtccagtgcc cggaatatcc ccttttacat ctggggaggg
     1261 ctcgccggca tgccccaagc atggaaaatg accgagggga aagtggctgg cggcatggtg
     1321 ggatcctcta tggtcaaggt cccgatcacc cccaagacga tccccttcat ggagaatctg
     1381 gtggccaaat acaaggtcgg ccccatcttc ggctcccaca cgacctatga caccctgttc
     1441 gggttcaaga aggccattga gaaggccggg ggcgcgagca atgtggaaaa tctcattaag
     1501 cagctagaac aggtcgagga ggtggcggtt ttcgggacca tcggatggga gcccaagtac
     1561 cattacaacc tcccctaccc gaaatacatc acccccgtcg tgcaatggca gaagggcgac
     1621 atgaaggtca tctttccggc ccagtacaag accggcgaat acctgccccc ggcagaggcg
     1681 aggaagtaac ccgtgtaggg gcgacccggc gggtcgcccc tacagcccgt cgaaatttta
     1741 taggtaacca acgccagcgc cttctatgat cgaaaccctc cattccatcc tcatctatac
     1801 cttcatctgg ggatcgatct atctccttat ttctctcggg ttctcgttga tctgcggcgt
     1861 tctgcggatc ttccacatgg ggtacggggc caccttcgtg ctggcggcct acgctgtctg
     1921 gaccttcatg gaggaggcga acctcggtct gatccccagc atccttctta tggccctggt
     1981 gcagatcggc ttttccctgg tggtcttcta ccggggggtc ttccagaggt acctggacca
     2041 ggaagaaatc ctcctcactc tttcgatcct gatcttcgtg accctgaccc acctggccaa
     2101 cttcctcttc ccggttacgg ccggagttag cattcccaca acgatcatcg acgacacgtt
     2161 ggtcatcgga aaagtgagga tcgcctacca gatgctcctg gcagccctgg ctggaatcct
     2221 ggttaccgcc ctctatgtcc tctggttcct caagacccgc acgggcctga tcattagggc
     2281 catcagccag aaccttcccg ccgcccttct tatgggagcc ggagtcaacc ggatgtacta
     2341 tctggccatg gtcctgtcgg tgatcccccc caccatctgc atgatgatga tcgcgccctt
     2401 ctggggggtc gatcccttca tgggctcccc gctgctgatg actgccatgc tcatcgccat
     2461 cctggggggt ctggggaacc tcaaaggttc gatcgtggct tcctacctca tcgggttcat
     2521 ccatgcctcg gtaagcttca tctacatctc ccgtttcatg ggactcgccg ccctagtggt
     2581 caccttggcg gtcatgatct accggagggg gggaatcttc gcgggagaga ctctatggta
     2641 aacctcgaac tccggaggga ccgggttctt ttccgctgga aggatcaaaa atacgagtgg
     2701 gtgatcgagc cccggtactg gcggaacccg gccatcggag tggccctcct ggcctccctg
     2761 cccctattcg tctcgccctt tccccacatt ttgaccatga tcaccacggc caacctgtat
     2821 gccgcagtca ctattcccct cggcctgcag attaccggga ctgcgcgggt caatttcggc
     2881 ccccagctct acctggggat cgggggatac gccgcggctc tgatgaacct ccacctcggg
     2941 gccagcccgg cagtgaccct gctgggaacc attctggtct gcggagggat cgccttcctg
     3001 ctgagcccca ccacctggat cgccaaaggg ctctattttt ccctcatcag tctcatcctt
     3061 cccctgacct ttctggatat cacctttatt ttcggggata tcttccgcgg agaagtaggt
     3121 ctcgtgggaa tgagccccct cctcagcctc cggaaggtga actggaatta catcgcctat
     3181 ttttaccttt ccctcggcct catgctcttc tatctcctgg tgtgcgacaa gatcggccgc
     3241 tcccgcttcg gggtaagcat ggcggccatc aatgaaaacg aggatgtggc ccggatgatg
     3301 ggggtgccgg tggaccggta caaggttttt ttctacgtct tcccctccat tctcaccggc
     3361 atcgtgggct ggtacatcgt gcacaccttc cggaccttcg ccggggtgac ctatctgccc
     3421 cttgaattca tgaccaagat cctgatcatc gtcatcgtgg gggggcgggc gttcctctat
     3481 ggcgccattc ccggggccta cctcattgcc gtggtggagg actccctgcg ggagttcggg
     3541 ccgatcaact atgtcctctt ccccctggtt ttgatcgccc tgatcgtctt tcttccccgg
     3601 ggagaaggcc tctggggcct ttaccataaa cgtcaccatc gggactatta tccggtcctg
     3661 cacgtgagga gagactagaa ggtgctgagg atcaaccggg tcagcaagag attcggcggg
     3721 ctttacgcgg tgaatgaggt cagcttttcc ctggaccaag gggaggtcat cggcctgatc
     3781 gggccgaacg gggccggcaa gaccaccctc ctcaacctga tctgcggggt gtataaggcc
     3841 gacggcgggt ccatcgaatt tttgggacag gagatcagcg ggatgccctc gaacaagatc
     3901 tgccgcctgg ggatttcccg gacctaccag atcccccagc ccttcagcgc catgactgcc
     3961 ctggccgggg tggccgtgtc agtcatcaac ggaaaacggc ggcccaatat gtgcctctcc
     4021 gacgcggcat tggaggcctc ctactacctg gaattcgtgg gcctcttctc caagcgggac
     4081 gccctggcgc gggaccttac cctttacgaa ctgcgcatgc tcgagctggc ccgggccctg
     4141 gccaccactc ccaaactcct gctgatcgac gaagtcatgg ccggcctgaa cccggccgag
     4201 gcttcccggg ctctggagat gatctggagc gcccaggagc agttcgggtt caccatcctc
     4261 tggatcgaac acgtcatgaa gattatcatg agcgcggccc agcgggttgt cgtcctccag
     4321 tatggaagga agatcgccga agggccgccc cgggaaatct cttcggaccc gaagatcatc
     4381 gaggcctacc tcgggagtcg acatgctaaa ggtcattgat tggaacgcct cctacggggt
     4441 catcccggtt ctccataacg tgtccctgga agtgaagccg ggggagatcg tggctcttct
     4501 gggatcgaac ggcgcgggca agaccaccct tctcaattcc atttccggaa tcgtgaaggg
     4561 gcgatcggga accatatctt ttttcgataa agggatcgga aaccttcccc ccaacaagat
     4621 tgtggaaacc gggatcgccc agattcccga gggaaggaag atcttcccct acctgacggt
     4681 gaaagaaaac ctcctcatgg gagcctgcat tctccaggct tggagggaaa gacagatcat
     4741 gctggagaag gtcaccgcgc tcttccccat cctgaaagaa cggtccggcc agcgggccag
     4801 cctcatgagc ggcggggagc aacagatgct ggtcatcgcc cggggactca tggcccgccc
     4861 ccgcctgctg ctcatcgacg agccgtccct gggcctgagc ccgatcatca tgggccagat
     4921 ttatgaggcg atcaagacct tcccccggga cggaatcacc gtgctcctgt ccgagcagaa
     4981 cgcgct
//
LOCUS       DASPRR010000639        23420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_305192, whole genome shotgun sequence.
ACCESSION   DASPRR010000639 DASPRR010000000
VERSION     DASPRR010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 23420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23420)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23420
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305192"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..347)
                     /locus_tag="VEL68_23390"
     CDS             complement(39..347)
                     /locus_tag="VEL68_23390"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HXZ38993.1"
                     /translation="MALVDQGNYGKSWETAAGYFKGAVTEKQWEQAVTGVRSPLGKVI
                     SRKIKSKQYATSLPGAPDGEYVVIQFETSFEKKKSSVETITPMKEKDGTWRVSGYYIK
                     "
     gene            complement(943..5439)
                     /locus_tag="VEL68_23395"
     CDS             complement(943..5439)
                     /locus_tag="VEL68_23395"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXZ38994.1"
                     /translation="MERRPESLTQFHPLIAGWFTDRIGHPTDVQRQAWPRIATGEHVL
                     ITAPTGSGKTLAAFLYSLNQLITGVWPTGQTSVLYVSPLKALSYDIQRNLLGPLQELK
                     KVFEGAGEAFPELRVFTRTGDTPQAERRRMLRHPPEVLITTPESLNLLLSSPSAKAIL
                     TRLSTVILDEIHAVVGNKRGVHLMTAVERLVRLSGEFQRIALSATLRPLEMMAEFVGG
                     FSLQGTPENPRYEPRRVITVRSALQKQYDLRIRYVPVMSEEEEPEDVWGPLVAGVKKI
                     ILQNRSTLIFVNSRRLCETLTLKINHGEKEPVAYSHHGSLSLEVRSEVERRLKAGELK
                     AIVATHSLELGIDIGSIDEVVLIQSPFSISSAVQRVGRAGHQVGEISRGTFFPTHPRD
                     FLEAAVLVPAILHHEIEEVKPILCPLDVLSQVIVSMVGVEAWKTDPLFAALKTSYPYR
                     FLSRGQFDLVLNMLSGRYAHTLIRELKPRVSIDRLDGTVSSRKGALQALYSSGGVIPD
                     RGYFQLRHLETKARIGELDEEFVWEASVGDTFSMGAQSWQVRQITHNDVLVSPAKSRG
                     DNAPFWKGEESGRDFHFSEGIGRFLEEADGRLNDPAFPASLQQERGMDEAAASHLIDF
                     LKRQKEETGCRLPHRHHLVAESISRGPGGAPGNMVVIHTLWGGRVNRPLAMALDTAWE
                     TRFGHRLEFFVGNDSVAILLPQEIHPEELVSLLDALRIDDLLRKRLEGSGFFGARFRE
                     CAGRALLIERTRWGERMPLWMSRLHSQKLLEAVLHFEDFPILLETWRTCLQDEFDLEN
                     LKKLLNELTSGVIAWTGVKTAHPSPFAGSDWWRQVSKYMYMDDRPLSDRVSRLRENFL
                     REIILQPGLRPTVSPELVKRFEAKRQRLSPGYSPACGRELLEWLKERLLIPRGEWEEL
                     MRGMQRDHGLDRDSLMEELKGKLIRIQPPEAGETLVAAREMVDLVLGHGYGRGKEVRA
                     ESWEGVPLFREGLPKEDEEGEDLSWFLGQWLSFYGPRNADFIGGTLGIGKDRLPAALE
                     DLLDARKLVQGELVSGGGAEDICDRENFEILVRLARAEAQPAFEPLKIDRLPLFMADF
                     QGITNPRGNLEGLRERIEQLLCYPAEAGIWESEVFPARLQPYDPSWLDTLMQEGDLQW
                     VGKEGHRIAFCFEPDLDLMREDSPQPTADAEEIPFPLGAAESGDLARLFPDPGGRYSF
                     AALLTRSPHSPSALAEELWNGVWQAQLTNDTFIALRRAIMTKFKPPDPGSAKDRRTPG
                     RWRHLRAGLREKKEGRSFPGHWHLLSSPVLPDDLLEIEERRKDRVRLLLERYGILFRE
                     LLQRELPSLSWSSVFRSLRIMELSGEVLAGYFFEGIPGPQFISPEAFRRLQRALPVEQ
                     VFWLNAADPASLCGIQLESIKGTLPPRLASTHLVYHGDRLAMVSKRYGRDLTFHIPSQ
                     DPRLPDYLGPLHHLLSRPVQPLRRIAIETINGEPAAQSPYLPVLRTSFKVIVDYKSVN
                     LSRS"
     gene            5959..6039
                     /locus_tag="VEL68_23400"
                     /pseudo
     tRNA            join(5959..5994,6002..6039)
                     /locus_tag="VEL68_23400"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /pseudo
                     /anticodon=(pos:5991..5993,aa:Ala,seq:ggc)
     gene            6525..7685
                     /locus_tag="VEL68_23405"
     CDS             6525..7685
                     /locus_tag="VEL68_23405"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ38995.1"
                     /translation="MERPEGKIMTHRERVVSALLRKQPDKVPIDLGSTINSSMVVEGY
                     EKLRRHFGHEFDTRLCNRMMRAVVMDEPVLQAMDIDTRGVFIGGPLKGGDQELGLDCY
                     RDFWGIERRKPEGGYYYDLVKSPLAGEISISDIMKYPWPDPDDPGFVQNLKPRVRWLR
                     ENTDAAIVLQLPPAFIHPSQFLRGFEDWYCDFAYHSKVMEPLFDAVLEITLQITKNAL
                     KEIGREVDVVCVAEDIGTQDRLMVSHDHYQRYIKPRHWKYFRQIRDLSSGKLLYHSCG
                     CITSIMEDLIDIGVEALNPIQVTARGMVPVELKKKYGRHLAFWGGIDVQYLLPKGTVQ
                     EVEQRVEELIEQMGGGGGYILSATHNIQPDIPLENVLAAFQHARAYSPSFGK"
     gene            7891..9129
                     /locus_tag="VEL68_23410"
     CDS             7891..9129
                     /locus_tag="VEL68_23410"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXZ38996.1"
                     /translation="MEQKSKRKGISRRTFLKGGVGAGLAVAGFPYVARAQELKKIKIR
                     AATILTSPSGVIEIYRLEQKLFEKYARKEGYDLELEWRDFPHGAAQVEAVLSGKLEIS
                     WWADIVQGRLISQKQPVHPIIMIEGYQNFCINVRPDSPIRNVEDLRGKTVGTVLGTVF
                     HGVIVNLLRSELGTADLKKLGINLINIPSVAQLMTMPTGIDAASNNIIFTLKGELEGM
                     SKPLVDAHGMTGNYYRGPAGEGAGHVLPGFKKAPFYPEAYYPFRGYHFATKKLIDEHP
                     KLIKAFIMATEEAVRNLKKMTKEDASQLVSKYWKLPPKEGGIVVGDVLPLIRGWSWIT
                     ESDARTIVEVSRFMAETGALPKPIEMKQVKENFEVVTPIMKAAYEEMDAFPKPEAFTD
                     ASAKDRRGLPLWMADKWSLS"
     gene            9191..10051
                     /locus_tag="VEL68_23415"
     CDS             9191..10051
                     /locus_tag="VEL68_23415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXZ38997.1"
                     /translation="MFKIGVDNFSYHRFFGWIIPGQSDPGKRWTLDDFLKRAVELKLD
                     VVSLETCFLPPLDRPFLESLKKKLADRKLEGILSWGHPRGLETGKNAAAAEDLKKTIE
                     AAKFLGTRVLRIVGGGFVDRAQEPVPLQKERVLRILPGLVEHAAAHDVVLAMENHQDF
                     TADEQVEILQKINSPYLRVTLDTGNNLRTLEDPIEVVRKLAPYTAATHIKDIIATGKG
                     NPATEWRRFWPSVPLGRGIIDLPEVIRILRQNHYPGALNVEIDFLDPRWPDEDEAVAE
                     SVAYLRKITA"
     gene            10121..11113
                     /locus_tag="VEL68_23420"
     CDS             10121..11113
                     /locus_tag="VEL68_23420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ38998.1"
                     /translation="MKNQFKTKKNLKWFGMAPLFDPKEGQTVIEPLSGGRGWWAGAPN
                     VLYDDQRRRFLISFRHRKPRELGRGFKACIAESEDGRKASIIWEGTKEELGSDSLERF
                     CIFKHPDGSFTLYASYVDPRDLRWRIDSVHANRPEKFDLRKRSQVLTAGDAKVEGVKD
                     PYVFLVGGIYYMFISYVPSPPKVDENLKRHMHETADVFNTGISRSHTALAISRDGRNF
                     EWAGDIFSPGQGWDGYATRIGSILHTPPGFTVFYDGSATVQENYEEKTGMAHTFDLKH
                     YQKLSVEGPVLVSPHSSGSLRYLDAVEVEGKVYYFYEYAREDGSHELRMNTVDL"
     gene            complement(11155..12318)
                     /locus_tag="VEL68_23425"
     CDS             complement(11155..12318)
                     /locus_tag="VEL68_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXZ38999.1"
                     /translation="MKNELLKTIPRIGVGILGYGFASRPHIVALKRMREVYWPPPAIP
                     ECVALCGRTEDKVKEAAARYEVKKWFTDWREMLKDPDIQVFDNCGANDVHAESCIEAA
                     KRGLHILCEKPLARNTEEAKKMLDAVRKARVKHMTGFNYRFVPAVRLAKELILQGVVG
                     EVHHFYGRFAVDRAMDPNIPLRWIYQKPLAGSGALGALGSHLLDLARFLVGEPVSVMA
                     QAKIFLKKRRLMGSSEWGEVTVDDAVSSLLEFENGAMGNFETSRFCPGRKNNQFFEIN
                     GSKGSIYFDLEEMNRLKVYLAQETIPSITGFHDVMVTEGSHPFFKEWWPTGHTLGWEH
                     TFIHQLKHFLEAIVKNTPVEPWGATLEDGYRNVVICDAMLKSAELGKKIRVRY"
     gene            complement(12442..13275)
                     /locus_tag="VEL68_23430"
     CDS             complement(12442..13275)
                     /locus_tag="VEL68_23430"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXZ39000.1"
                     /translation="MSQPKLKIERLKKEFWKSHSTGPVVAIEEFSLDVMEHEFVAIVG
                     PSGCGKSTLLRIVAGLERPTEGIVLLDGREITGPSPQRGMFFQEYALFPWKTVWGNVE
                     FGLKAQNVVREERNRIIEEYLKLVGLKGFEKHYPDEISGGMKQRCALARVLATDPAIL
                     LMDEPLAAVDAQTRLILQEDLLRIWGEDRPRERRKTVFYVTHNIDEAVLLADRVVVMS
                     AHPGRIRKILPINLPRPRIQARTLPEFQVHIQQIWDLIKAEAYRATLKVSGVNQDASD
                     L"
     gene            complement(13288..14058)
                     /locus_tag="VEL68_23435"
     CDS             complement(13288..14058)
                     /locus_tag="VEL68_23435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ39001.1"
                     /translation="MAVGLIKKSWLLALIFLFWQMAITFGTPGKMTPTPLGAGKGLLE
                     LAQTGALLKALIISLTRVFWGFGAALLLGVSLGILMGGVKAVERWLDPIIESLRPIPP
                     IAWVPLAIIWFGLGDTAPAFIVALAAFFYILINTIAATKNVDRQLIEAAHTLGASRWL
                     IVREVIIPSALPLILVGARLGMGAAWMSIIAAELTAGSRVWAGQLEAGIGELMYRFFA
                     YEPNLNYVVSCMIVIGFIALAIDGVFRLLRRSLIRWRP"
     gene            complement(14087..14866)
                     /locus_tag="VEL68_23440"
     CDS             complement(14087..14866)
                     /locus_tag="VEL68_23440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ39002.1"
                     /translation="MNLLREKAQSALSYFRTMTLFVILWYLLSRWIENRLLLPSPPEV
                     AGALWDLLSEQHFLLDIFISLKRLILGYLVAGAVGIPLGFLLGLSRQAESIVSPVVEV
                     LRPISPIAWIPIAMFLFGVGDGLAIYIIFYGASFQFLLNTIAGVKSIDRRLFEVSLTV
                     GANRWLIIRDVILPGTLPLIIVAGRLAMASAWMSIIAAELVGAPNGLGFKIEWAHSMF
                     MSESIVAGMATIGILGYLSDLLLRWIGRKAIPWRKTSYGLG"
     gene            15035..16009
                     /locus_tag="VEL68_23445"
     CDS             15035..16009
                     /locus_tag="VEL68_23445"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ39003.1"
                     /translation="MKKGIQMFILSLAITAGIGFSTPRPTLATYPEKPVTMICVFPAG
                     GAMDMTARALAEAAKKYFPKPFAVMNRPGAAGTIGTAEAIQARPDGYTIGITAVAVLT
                     VQPHRTKLPFGPPEDYTPIIKLVNQPVVLAVKQDAPWKTIQEFIQFGKANPGKLRVGC
                     PGIGTIPMLDAEQFKMMSQADLTTVPFAGGAESVPAMLGGHVEAVSQHPSEVMAQVKA
                     GKARVLVVFEEKRNALFPDAPTSKELGYDITMGVYYMLIGPKGLSSQIIALIHDAAKK
                     SMEDPIFTKPMEARGFDITYEGPQDLKKRLVRDYEGNAKFADLLKEKK"
     gene            16013..16477
                     /locus_tag="VEL68_23450"
     CDS             16013..16477
                     /locus_tag="VEL68_23450"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ39004.1"
                     /translation="MSLRALPIVSLCFLLLGIAYLVDNSKLPMGSAAKPGAGLFPLLV
                     GFALSGLSLALFIWAGRNQDTAAKGQEPFPVGKDGQRVLALGVVLALFVLLLKPLGYG
                     ICGTALLAAVLRLLGLRSWRRIILISLVAAATSFFLFDTLLGVPLPRGVFFS"
     gene            16479..17981
                     /locus_tag="VEL68_23455"
     CDS             16479..17981
                     /locus_tag="VEL68_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ39005.1"
                     /translation="MDSLQGLAYGFGVALTGANLLACLLGVLVGTVVGVLPGIGPIGA
                     MALLIPSTFSLGPTTALIMLAGIYYGSMYGGSTTSILVNVPGEAASVVTAIDGYQMAR
                     RGRAGAALAVSAVGSFVAGTLGIVALMLFAPPLAELALEFGPPEYFALTLVGLFFLSG
                     LGGGSRLKSFLMVAFGLLLGTVGMEPISGLPRFTLGQLSLAQGVELVPVAMGLYGIGE
                     VLIIAEKITGIPELIKVKFRELFPTVAEWKRAFPPMLRGGGVGFLIGLIPGPAAILST
                     FASYALEKRVSRHPQEFGKGAIEGVAGPEAANNSATAGAMVPLLALGIPFAPATAMLI
                     GALIIHGVQPGPLLMTQQPEIFWGVVASMYIGNLVLLILNLPLVGAFASILRLPQHLL
                     MGLILLLCLVGTYSVNNSLLDLYILIGMGGVGYFLRKLKFDMAPLILALVLGPMMEKT
                     LLQSLFMARGDVWSILSRPIPAVLYSIAIAVMVLPLLYRWVKKRSEALRP"
     gene            18314..19093
                     /locus_tag="VEL68_23460"
     CDS             18314..19093
                     /locus_tag="VEL68_23460"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXZ39006.1"
                     /translation="MSEIRFPSMSLSGKIAIVTGAGQGLGLWMAQGLAHAGADTAIAE
                     INPQAGEAAAEEVRKIGRTALFIETDVRRGDSIRAMMDRVVKEWGRIDILVNNAGVNV
                     RKKVEEVEPEDFDEVASVNLRGAYFCAQAAGKVMIQQRQGKIINIASAAGFLVRSGIP
                     NSVYSITKAGVIMMTKALAAEWSPYNVNVNAVAPGYFVTPLTVPRFKDPKAWQAIMDS
                     TPLKRVGQAEDIIGPVVFLASDAAAFITGACLFVDGGRTVL"
     gene            19326..20567
                     /locus_tag="VEL68_23465"
     CDS             19326..20567
                     /locus_tag="VEL68_23465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXZ39007.1"
                     /translation="MVQGEFPLLHWKGSGLTTRTKYFVVALLYFSEGLPFGLINNLLS
                     VYFRVKRISLEEIGLLSLLGFAWSVKILWAPLVDRFGRRYFWILPAQLLLACFSAVLA
                     FIDPVSQKALFWLALAGVCLASATQDIAADAYSIDILEEKELGPANGVRSAAYRVALI
                     TAGGVLVMLSETMGWSLTFIAAGVIMAVLALAVVSFPSFRQKRPVVSASLPLWEQYVD
                     PIKRLFLKPNFWTAVIFILFFKVGEAMLVAMANPFWIDQGFTPGEIGFAVGTLGTLAS
                     IVGAIGGGGLTARWGIRKALWILGAVQATASLGYAFASLPLAPRFSIYFAALLESLAI
                     GLATAAFLSFLMKLCEKKFSATHYAFLTILFGLGRSLAGVLGGYSASFLGYSVFFFVT
                     FIIGLAPLPLILYLQPTLNKG"
     gene            20626..21738
                     /locus_tag="VEL68_23470"
     CDS             20626..21738
                     /locus_tag="VEL68_23470"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXZ39008.1"
                     /translation="MVRRDLRDKIAQMIMVGFREAVVDKDTPVVRAIRDHGLGGIILY
                     NIDLKSFLEAQKKKPDLDRTEGAKLCPKNILSRGQLKTLTSALKSYSKTPLLISADQE
                     GGMVSRLGPAAGFAERLSPRALGEKDDPPATVKEAEAMAQDLWETGINLNLAPVVDLN
                     LNPEGLIARMGRSFGADPDLVCRHAEVFIQAHRERGILTTLKHFPGKGSVGKDTHFEL
                     ADVTSLYQDQELQPFSRLIREGRADLVMTSHINHRRWDEDYPVTLSPRILQGLLREKL
                     GYRGVIISDDLLMGAIIKQFSLEEACVLAVQAGVDILLASNNAPEGDNPDLFSHIFEA
                     LLRAVEEGRIAPHLIESSNSRILTVKKSLPPSSLSA"
     gene            complement(21840..22637)
                     /locus_tag="VEL68_23475"
     CDS             complement(21840..22637)
                     /locus_tag="VEL68_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HXZ39009.1"
                     /translation="MDREKLRDLPPREVRQLIREGKWRKGTAFVSAGYAQANLAILPR
                     NLAFEFLLFCQRNPKPCPVLEVTEPGNPILSQIAKGADIRTEIPKYRIYEKGKYLREV
                     ETIKDYWRDDLVAFLIGCSYTFESAMAKANIPLRHVEDKKDVSVYITNIPCAPAGVFS
                     GPMVVSMRPIPEKLVVRAIQVTSRFPATHGAPVHIGDPKKIGIQDLQKVDWGDPLRFE
                     KDDVPVFWACGVTPQAVAMASKPEIMITHSAGHMFISDIRDEELAAL"
     gene            complement(22725..>23420)
                     /locus_tag="VEL68_23480"
     CDS             complement(22725..>23420)
                     /locus_tag="VEL68_23480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXZ39010.1"
                     /translation="LGMLVGAILAVGLSLLVAIPCFRLAGHYFAIATIAIGEICQIGM
                     VNWKGGGGANGLYLPILEEGFLNLEFHNTKVPYYYIALGMAAITFATVFFLERSFLGY
                     YFRAIKGDLDASRAVGIHVARYKTVALGISAFFTSIAGTFYSQYVLFIDPESVLPLML
                     SVLISLVAILGGVATVWGPFLGAAVLIPLSEFTRTYLGGRGTAVDLMIYGFLIILIAR
                     FQPTGLMGLLKKR"
BASE COUNT         5089 a         6620 c         6638 g         5073 t
ORIGIN      
        1 gaggagcctg ggtcggggac tcggcgaaat caatattcct atttaatgta ataccccgac
       61 acccgccagg ttccgtcttt ttccttcatg ggagtgatgg tttcgacgga agatttcttc
      121 ttctcaaagg aagtctcaaa ctggatcacg acgtattcac catcgggggc accgggcaga
      181 gatgtagcgt actgcttcga tttgatcttg cggctgataa cctttccgag gggtgatcgg
      241 accccggtca ctgcctgttc ccactgtttt tcggtgactg cccccttgaa gtatcctgcg
      301 gctgtctccc agctttttcc ataattcccc tggtctacca gggccaacta attcatggcc
      361 gcttccacag cctttccctg gtccttcgaa tctgccgggg atgattgtac aaagccaaga
      421 gtaaagaaga gaaataggat caaacctggc aaaactttat tcttcttgcc catcgattta
      481 ccctcccttc ttttatttaa ggcatctgat tggggattac ggtttccttt ctcagtcctc
      541 gcgaaggttt tttttaaaat ctctcggcca aaacccggaa atctttatca ttttgatccc
      601 ccaaaaaata ggtcccccga gaagcaaccc cagtctgatc ctcagggaac cctatcctgg
      661 aaaccaaggg tgggcggcct tttccggata cccctatacg aagattgttg ataaataggg
      721 ctttatgaga cccgatataa ttgagggcaa taagggcgcg ggttggttct catactgatc
      781 atattccatt gaatttattg cataattttt tggttcaaaa atgggcagtc tgtagaaaaa
      841 caaatgtata tgcctcctta ccagtacctc atcacaactt ttccacaact tatcaacaga
      901 attttgtgga aagagaaaat aggcccggga aaacaaggaa ttctaggatc gggagaggtt
      961 cacggattta taatccacaa tgaccttgaa ggaggtgcgc aggacgggga gataggggct
     1021 ctgcgcagcg ggttctccgt tgatggtctc gatggcgatt ctccttaatg gttgaaccgg
     1081 gcgactcaaa aggtggtgca aagggccgag ataatccggc agacggggat cctgcgaagg
     1141 aatgtggaaa gtcaagtctc ggccataccg ctttgagacc atggccagcc ggtccccatg
     1201 gtaaaccaag tgcgtgctgg ccaggcgggg gggcagcgtt cctttgatag actccagctg
     1261 aatcccacag agagaagccg ggtccgcggc attgagccaa aagacttgtt ccaccggcag
     1321 cgcccgctgc aggcgacgaa aagcctcagg ggaaatgaat tgcggcccgg ggataccttc
     1381 gaagaagtaa ccggccagga cctctccgga taactccatg atccgcaaag accggaagac
     1441 gctcgaccag ctcaaggagg ggagttctct ctggaggagt tcccgaaaaa gaattccgta
     1501 ccgttccagg agcagccgga cccggtcctt cctgcgctcc tcgatttcca gcagatcatc
     1561 gggaagcact gggctggaaa gcaggtgcca gtgtccgggg aaggaccttc cctccttttt
     1621 ctctcgaagg ccggcccgaa ggtgcctcca gcgaccggga gttcttctgt ccttggccga
     1681 gcccggatcg ggcggtttaa atttggtcat aatcgcccgg cgcagggcaa taaaggtatc
     1741 attcgttagc tgcgcctgcc agactccatt ccataattct tctgccaggg ccgacgggga
     1801 atggggcgac cgggtcaata aggcggcaaa ggaataacgc ccgcccgggt ccggaaacag
     1861 gcgcgccagg tctccgctct ccgcggctcc taggggaaaa ggaatctctt ccgcatcggc
     1921 cgtcggctgg ggtgagtcct cccgcatcag gtcaaggtcc ggttcaaagc aaaaggcgat
     1981 ccgatggcct tccttcccca cccactgcaa gtccccctcc tgcatcaggg tgtccagcca
     2041 ggaagggtca taaggctgaa gccgggcggg aaagacctcg gattcccaga ttccggcctc
     2101 ggccggatag cagagcagtt gctcgatccg ctcgcggagt ccctcgaggt ttcccctcgg
     2161 gttcgtgatc ccctggaaat ctgccataaa cagagggagc cgatcgatct tcaggggttc
     2221 gaaagcgggt tgtgcttcgg cccgggccag tcggacaagg atttcaaaat tctccctgtc
     2281 gcagatatcc tcagccccgc ccccgctgac cagctctcct tggaccagct tccgggcatc
     2341 tagcaggtct tccaacgccg ctggcaatcg atcctttcca atccccaggg tgccgccgat
     2401 gaaatccgca ttccttggcc cataaaatga aagccactgg ccaagaaacc aggagaggtc
     2461 ctccccctcc tcgtcctcct ttggcagccc ttcccgaaaa agagggactc cctcccagga
     2521 ttcagcacgc acttcttttc ccctgccgta gccatgaccc agaacgaggt ctaccatctc
     2581 ccttgccgca accagagtct caccggcctc cggtggctga atccgaatca gcttgccctt
     2641 caactcttcc attaaagagt cccgatccaa gccatggtcc cgctgcatgc cccgcataag
     2701 ctcttcccat tctcctctgg ggatgagcag gcgttctttg agccattcga gaagttcacg
     2761 gccgcatgcg ggagaataac ccgggctcag cctctgtcgt ttagcctcga atcgtttcac
     2821 cagttcaggg gagaccgtgg ggcgcaaccc cggctggaga atgatctccc ggaggaagtt
     2881 ttcccgcagt cgcgaaaccc gatccgacaa gggccggtcg tccatgtaca tgtatttgct
     2941 cacttgccgc caccagtcgc tcccggcgaa ggggctggga tgggcagttt tcacccccgt
     3001 ccaggcgatg acccccgacg tcagttcgtt cagaagcttc ttcagatttt ccaggtcgaa
     3061 ctcatcctgc aggcaggtac gccaggtttc caggaggatc gggaaatcct cgaaatggag
     3121 gacggcctcc aatagcttct gggaatggag ccggctcatc cagaggggca ttcgctcccc
     3181 ccaccgggta cgctctatga gcaaagccct ccccgcgcac tcccgaaacc gggctccaaa
     3241 aaagcccgac ccctccagcc gctttctcag gagatcatcg atccgaaggg catccaggag
     3301 ggagaccaac tcttccgggt gaatctcttg gggaagtaaa atggccacgc tatcgttccc
     3361 cacgaaaaac tccaaccgat ggccgaacct cgtttcccag gctgtatcca gggccatagc
     3421 caggggccgg ttcacccgcc ctccccagag agtatggatc accaccatat ttcccggggc
     3481 tccgcccgga cccctgctaa tggactctgc caccagatga tggcgatggg gcagacggca
     3541 gccagtttcc tctttctgcc tcttcaaaaa atcgatcaga tggctggctg cggcttcgtc
     3601 cattccgcgt tcctgctgca gggaagccgg gaaggcagga tcattcagcc gaccatcggc
     3661 ttcctccagg aatcttccga tcccctcaga aaaatggaag tcccggccgc tttcttcacc
     3721 cttccagaaa ggagcgttat ccccccgtga ttttgccggc gataccagca cgtcattgtg
     3781 cgtgatctgg cggacctgcc agctctgggc ccccatggaa aaggtatccc ctacagaggc
     3841 ctcccagaca aattcctcgt ccagctcccc gattctggcc ttggtctcca ggtggcgaag
     3901 ttggaaatac ccgcggtcgg gaatgacccc gccggaggaa tagagggcct ggagcgctcc
     3961 tttgcgcgaa ctcacggtgc catcgaggcg gtcgatggag actctcggct tcagctcccg
     4021 gatcagggtg tgggcgtaac ggccggaaag catgttcagc acgaggtcga actggccgcg
     4081 gctcaaaaaa cggtacggat agctggtctt caaagcggca aagagcgggt ccgtcttcca
     4141 ggcctcgacg cccaccatgg atacgatcac ctgggacagg acgtcgaggg gacagaggat
     4201 gggtttcacc tcttcgattt cgtgatgaag gatcgccgga accaggaccg cggcttcgag
     4261 aaaatcccgg gggtgggtgg ggaaaaaggt tccccggctg atctccccca cctggtggcc
     4321 ggcccttcct acccgctgga ccgcggagga gatggaaaag ggggattgaa tcaataccac
     4381 ttcatcgatg gatccgatgt cgattcccag ctccagggaa tgagtcgcca cgatggcttt
     4441 gagctctccg gccttcagcc tccgctctac ttcgctgcgg acctccaggg agagcgaccc
     4501 gtggtgggaa taggccaccg gctctttttc cccgtgattg atcttgaggg tcagagtctc
     4561 gcagagacgc cgactgttga cgaaaataag cgtcgatcgg ttctgaagga tgattttctt
     4621 caccccggcg accaggggtc cccatacatc ttccggttcc tcctcttccg acatcaccgg
     4681 aacgtagcga atgcgaaggt catactgttt ttggagtgcg gagcgcaccg tgatgacgcg
     4741 ccgtggctcg tagcgggggt tttccggtgt tccttgaagg ctgaacccac ccacgaattc
     4801 cgccatcatc tccagaggcc ggagggtcgc cgaaagggcg atccgctgga attctccgga
     4861 cagacggacc agccgctcca ccgccgtcat cagatgaacc ccccgtttgt tccccacaac
     4921 ggcatgaatc tcgtcaagaa tcaccgtgga cagccgagtg aggatggcct ttgccgatgg
     4981 agaactcagg agcaggttta aactttcggg ggtggtaatc agaacttccg ggggatggcg
     5041 cagcatccgg cgcctctcag cctggggcgt atccccagtc cgggtgaaca cccggagctc
     5101 tggaaaagcc tctcccgccc cttcaaagac cttcttcagc tcttgcaagg ggcccaatag
     5161 gttgcgttga atatcatagc tgagggcctt caggggagaa acgtaaagaa cgctggtctg
     5221 gccggtcggc catactccgg tgatgagctg gttgagtgag tacaagaagg ctgctagggt
     5281 cttcccgctc cccgtgggag ccgtaatgag gacgtgctct cccgtggcga ttctcggcca
     5341 ggcctgccgc tggacgtcag tgggatggcc gatccggtct gtaaaccacc cggcgatcag
     5401 gggatgaaac tgggttaagc tttccggtct tcgttccatt gaaaggaatc cctctttcca
     5461 atcatacggt attctaaatt caagtctcca ccccgtcaag gaaagttcat agcttgccca
     5521 cccccagttc ttgaaaaaga gtccttcgaa aataagatct ctggaaagga ggggggatct
     5581 catggggttc agggcggagg aatttggatc atcatcctaa ggcggtggtt cttccattgg
     5641 atcgggcctg tcctgggggg ggttgagacg ggaaaatgct ttggttctct attctgcttg
     5701 gaggaatctg ctctcaggcc tctgcctgcg gaacatgggg aggggtttca tggaaggata
     5761 agcacccgcc gagcctgatg atccaaattc ctccaccctg aaccccaaca agcacaaaaa
     5821 actgggggtg ggcaagaagt tcatagccca tccagcgttg ggcaaaccct ttggagctat
     5881 tcttgcattt aagtttgaaa ttcatctgcc gaaagactat tcggatgttg cgtttcacgt
     5941 tgttcatccg gatatcttga ggctgtggtg tattgggagc acgcataatt ggcattcatc
     6001 cattatgagg tagaggttca attcctctca gcttcaccaa atattttgat gggcaaggcc
     6061 ataaatgagt tggccttttt ttatttaaag cccttcttat gcttgccttt tatccgtaaa
     6121 catcaacaat gaagagtaac ctctactgaa tagcaggtga agtatccaaa atcccgctgg
     6181 cggatttacc cccaacctcg attcttcctt cctagacttt tttcctatat ttggcctgtc
     6241 tatacaacct ttccatccca aatgctcgat tttcatcatg ctttgcacgt agagattttt
     6301 ctatttaatc cacaaccttc ccctccaaaa agctgatcaa gggggagcag gggagcggga
     6361 atagttcaga ttttcccttg atcaggtccc aaattgacaa tcagcccggt ttctcctaat
     6421 attggtctcg cgtatcactc ggtctcgttt cctgaaactt cttcaggggt aaatttccca
     6481 ttttttaatt ttttcatgcc ttctcctggc caggggaaga gtggttggaa cgaccggaag
     6541 gaaaaattat gacccaccgg gaaagggttg tcagcgcgct tttgcgcaaa cagccggaca
     6601 aagtccccat tgacctggga agcaccatca acagcagcat ggtggtggag ggatacgaaa
     6661 aattaaggag gcattttggg catgaattcg acacccggct ctgtaaccgg atgatgaggg
     6721 cggttgtgat ggacgagccc gtgcttcaag ctatggatat cgatacccgc ggggtcttca
     6781 ttggagggcc tctcaaggga ggagatcagg aactagggct tgactgttac cgggacttct
     6841 gggggatcga gcggaggaag cctgaaggtg ggtattatta cgacctggtg aagtctcccc
     6901 tggccggaga gatttccata tccgatatca tgaagtaccc ttggccggac ccggacgacc
     6961 ccggattcgt gcagaacctc aagccgaggg tcagatggct ccgcgagaac actgatgccg
     7021 ccatcgtcct gcagcttccc ccggctttca tccaccccag ccagttcctg cgggggtttg
     7081 aagactggta ttgcgatttc gcttatcact ccaaggtgat ggagcccctc ttcgacgcgg
     7141 ttctggaaat cactctacag atcaccaaga acgctttgaa agagatcggg cgggaggtgg
     7201 atgtggtctg cgtggccgag gatatcggca cccaggaccg gcttatggta agtcacgatc
     7261 attatcagag atacataaaa cctaggcatt ggaaatactt ccggcagatt cgggatcttt
     7321 cctcgggcaa actgctctac cacagctgcg gctgcatcac cagcatcatg gaagacctga
     7381 tcgacattgg cgttgaggca ctcaacccaa tccaggtcac tgcaaggggc atggttcccg
     7441 tagaactcaa gaagaaatac ggccgtcacc ttgctttctg gggtgggatc gacgttcaat
     7501 accttctgcc caaggggaca gtgcaggagg tggagcaaag ggtggaagaa ctcattgagc
     7561 agatgggcgg cggaggggga tacatcctct cggcaaccca taacatccag cctgacattc
     7621 ccctggaaaa cgttctggcc gcctttcagc atgcgcgggc gtacagcccc tcgtttggga
     7681 agtaagacct taccaagtgc ggattgcgga ttggaaaaat aagccgcgct cacccggctc
     7741 gttctggctt tcctagagac tcactccccg gccctcgcga gccgaaaaca attgacaatg
     7801 cacccgctta agatacaatc cggattgaag ttccggaaaa agtttgataa accctgcatt
     7861 ggattcatac aggaaaggag gctttcgccg atggaacaga agagcaaacg aaaagggatc
     7921 agtcgtcgaa cattcctcaa gggaggagtg ggagcgggct tggcagttgc cgggttcccc
     7981 tatgtggcca gggcccagga gctgaaaaaa attaaaatcc gggcggccac gattctgacc
     8041 tctccttcgg gggtgatcga gatctaccgc ctcgagcaga agctctttga aaagtacgcc
     8101 cgaaaggaag gatatgacct ggagctggag tggcgggact tcccccacgg ggcggctcag
     8161 gtcgaagccg tcttgtccgg caagctggaa atctcctggt gggcggacat cgtgcaggga
     8221 cgcctgatct cccagaagca gcccgtccat cccatcatca tgatcgaggg ctatcagaac
     8281 ttctgcatca acgtccggcc cgattccccc atccggaacg tagaggacct gcggggaaag
     8341 accgttggca cggttctcgg caccgtcttc cacggagtca tcgtcaactt gctgaggagc
     8401 gagctgggga cggccgacct caaaaagctg gggatcaacc tgatcaacat cccctcggtg
     8461 gctcaactta tgaccatgcc cacaggcatc gacgccgcga gcaacaacat catcttcacc
     8521 ttaaaggggg agctggaggg gatgagcaaa cccctggtgg acgcccacgg gatgaccggc
     8581 aactattaca ggggaccggc cggggaggga gccgggcatg tcctcccggg cttcaaaaaa
     8641 gcccccttct atcccgaagc ctattacccg tttcggggat accatttcgc taccaagaaa
     8701 ctgatcgatg agcatcccaa gctcataaag gccttcatca tggccaccga ggaggccgtc
     8761 cggaacctga aaaaaatgac caaagaggat gcttcccagc tcgtcagcaa gtattggaag
     8821 ctcccgccga aggagggggg aatcgtggtg ggcgacgttc tccccctgat acgcgggtgg
     8881 agttggatca cggagagcga cgcccgcacg attgtggagg tgtccaggtt catggccgaa
     8941 accggcgccc tgccgaagcc gatcgaaatg aagcaggtta aggaaaactt cgaagtcgtg
     9001 actcctatca tgaaagcggc gtacgaagag atggacgctt tccccaaacc ggaagctttt
     9061 accgatgcca gcgcaaagga tcggcgaggc ctgcctctct ggatggcgga taagtggagc
     9121 ttatcgtaaa acggcggaaa atttataaat ggtccctggg ggattttggg ggagggatgg
     9181 agcgaggctt atgttcaaaa tcggagtgga taatttttcc tatcaccggt ttttcggatg
     9241 gattattccc gggcaatcag accccggaaa gcgctggacc ctggatgatt ttctgaagcg
     9301 ggccgtggag ctgaagctgg acgtggtttc cctggagacc tgcttcctgc cccccctgga
     9361 ccgtcccttt ctggagagct tgaaaaaaaa gctcgccgac cggaaactgg aggggatact
     9421 ctcctggggc cacccgcggg ggctggagac cggaaagaat gcggcagcgg cggaggatct
     9481 aaagaagacg attgaagcag ccaagtttct ggggacccgc gtgctgcgca tcgtgggggg
     9541 agggttcgtc gaccgggccc aggagcccgt cccgctgcaa aaggaacggg tcctaagaat
     9601 tctccccggc ctcgttgagc atgccgcagc ccatgatgtg gtgctggcaa tggaaaacca
     9661 ccaggacttc actgcggatg agcaggtgga gattctccag aaaatcaatt ctccctacct
     9721 gcgggtgacc ctggatacgg gaaacaacct gcggactctg gaggatccga ttgaagtcgt
     9781 caggaagctg gctccctata ccgctgccac ccacataaag gacattattg ccacgggaaa
     9841 gggaaatccg gctaccgaat ggcgccgctt ttggccgtcc gtccccctgg gcaggggaat
     9901 catcgattta ccagaggtga tccgaattct ccggcagaac cattaccccg gcgccctgaa
     9961 tgtagagatc gatttcctgg acccccgatg gcccgatgag gatgaggcgg tagccgagag
    10021 cgtcgcctac ctgagaaaga ttaccgcctg attgaaagcg ggattcagaa atgcgcaatg
    10081 cgccttttac tctacgcgat gccgggtgga atgtggagcc atgaagaacc agttcaaaac
    10141 caagaaaaac ctgaagtggt tcgggatggc acccctcttc gatccgaaag aggggcagac
    10201 cgtgattgaa cccctttccg gcggccgggg atggtgggcc ggggccccga atgttcttta
    10261 cgacgatcag aggcgtcgtt ttctgatttc ctttcggcat cggaaaccaa gagagctggg
    10321 ccgggggttc aaggcctgta tcgccgaaag tgaggacggc aggaaggcct ccattatctg
    10381 ggagggaacc aaggaggagc tggggtctga ttctttggag agattctgta tttttaaaca
    10441 ccccgatggt tcctttactc tttatgccag ctatgtggac ccccgcgatt tgcgctggcg
    10501 gatcgactcc gtccatgcca atcgtccgga aaaattcgat ctgcgcaaga ggtcccaggt
    10561 cctcaccgcg ggggacgcaa aggtggaagg ggtaaaagac ccctatgttt ttctggtggg
    10621 tggaatctac tacatgttta tcagttatgt ccccagccct cccaaggtcg atgagaattt
    10681 gaaaaggcac atgcatgaaa cggccgatgt cttcaacacc ggcatctccc gctcccatac
    10741 cgccctggct ataagccgag acggcaggaa tttcgaatgg gcgggggata ttttttctcc
    10801 cgggcaggga tgggatgggt atgccacccg gatcgggtcg atcctccaca ccccgccggg
    10861 gttcacggtc ttttatgacg gatcggcgac ggttcaggaa aactacgaag agaaaaccgg
    10921 catggcccac acctttgacc tgaagcatta ccaaaaattg agcgtggagg ggccggttct
    10981 ggtctccccc cattcctcgg gctctctccg ttatttggat gcagtggaag tggaaggaaa
    11041 ggtttattat ttctacgaat atgcccggga agacgggtcc cacgaattgc ggatgaacac
    11101 cgtggatctc tagcaaaaag gtaggggcgg gtttgaaccc cgcccctatc cctttcaata
    11161 tctcactcta attttcttcc ccagctctgc cgacttcagc atggcgtcgc agatcaccac
    11221 attgcggtag ccgtcctcca gggtggcccc ccagggttcg acgggcgtgt ttttcacgat
    11281 cgcttccagg aagtgtttca gctgatggat aaaggtgtgc tcccagccta gggtgtgccc
    11341 ggtgggccac cactccttga agaaggggtg gcttccctcg gttaccatca cgtcgtggaa
    11401 cccggtaatg gaaggaatgg tctcctgggc aagatagacc ttcaagcggt tcatctcctc
    11461 caggtcgaag tagatcgacc ccttggaccc attgatttca aaaaactggt tgttttttcg
    11521 gcccgggcag aaacgcgaag tttcaaaatt tcccatggcc ccgttttcaa attcaaggag
    11581 agaagaaacg gcgtcatcta cggtcacctc gccccattcg gacgacccca tgagtcttct
    11641 tttcttcaga aagatcttgg cctgggccat gacggacacc ggctcgccca cgaggaagcg
    11701 ggctaaatcg aggagatggg atcccagcgc tcccagggcg ccgcttccgg ccaggggctt
    11761 ttggtaaatc catcgcaagg gaatattcgg gtccatggcc cggtccacgg cgaagcggcc
    11821 gtagaaatga tggacctcgc cgacaacgcc ctgcaggatc aattccttgg ccagccttac
    11881 cgcggggacg aaccggtagt tgaagccggt catgtgcttg acccgggctt ttcggaccgc
    11941 atccagcatt tttttggctt cttcggtgtt gcgggccagc ggcttctcgc aaaggatgtg
    12001 cagccctctc tttgccgctt cgatgcagct ttccgcatgg acatcgttgg ccccgcaatt
    12061 gtcgaagacc tggatgtccg ggtccttcag catctcccgc cagtcggtga accacttctt
    12121 gacttcgtag cgggccgccg cttccttcac tttatcttcc gtccgcccgc agagggcgac
    12181 acattcgggg atggccggcg gaggccaata cacctcccgc atccgcttca gggcgacgat
    12241 atgagggcgg ctggcaaatc cgtatcctag gatccccacg ccgattctcg gtatggtctt
    12301 gagaagttca tttttcattt tccagactcc tctatcgttt tcaagattat ggaacgaaga
    12361 gcattcccca tccgcaggga caattcatga attgcccctg cagtgggatt cgcgccgcag
    12421 gggcggtttt gaaacccgtc cctacaaatc cgacgcatcc tgatttactc cgctcacctt
    12481 gagcgtcgcc cggtaggcct cggccttgat caagtcccag atctgctgga tgtggacttg
    12541 gaactcgggc agggtccgag cctggatccg gggccggggg aggttgatgg gaaggatttt
    12601 ccggatccgc ccgggatggg cgctcatgac caccactcgg tcggccaaaa gaaccgcctc
    12661 gtcgatgttg tgggtcacgt aaaacaccgt ctttctccgt tctctgggtc gatcttcccc
    12721 ccaaattcgc aggaggtcct cctgcaggat caggcgcgtc tgggcatcca ccgccgccag
    12781 gggttcatcc atgagaagaa tggccgggtc cgtggccagc acccgggcca gggcgcaccg
    12841 ctgcttcatt cccccggaga tctcgtcggg atagtgcttt tcgaagcctt tcagccccac
    12901 cagtttgaga tattcttcga tgattcggtt ccgctcttcc cgaaccacgt tctgggcttt
    12961 cagcccgaac tccacgttcc cccacaccgt cttccaggga aaaagggcgt attcctggaa
    13021 gaacattccc cgctgcggac tggggccggt aatctccctt ccgtccagga gcacgatacc
    13081 ttcggtcggt ctctccagcc cggccacgat ccggagcagg gtggatttcc cgcacccgct
    13141 gggccccacg atcgccacga actcgtgttc catcacgtcc agactgaatt cttcaatggc
    13201 caccaccgga cccgtgctgt gggatttcca gaattccttt ttcaatctct cgattttcaa
    13261 cttcggctgg gacatgattg atctctccta agggcgccac cggatgaggc tgcggcgcag
    13321 aaggcggaaa acgccgtcga tcgccagggc aatgaagccg atgacaatca tgcagctgac
    13381 cacataattc aaattgggct cataggcgaa gaaccggtac atgagttctc cgattccggc
    13441 ttccagttgt cccgcccaga cccgggagcc ggcggtcaac tcggcggcga taatggacat
    13501 ccacgccgct cccattccca gccgggcccc gaccaggatg agcggaaggg ccgatgggat
    13561 gatcacctct cgaacgatca accagcggct cgctcccagg gtatgggccg cttcgatcag
    13621 ctggcgatcc acgttcttgg tggcggcaat ggtgttaatg aggatgtaga agaaagcggc
    13681 cagggccacg atgaaagccg gggccgtgtc tcccaagcca aaccagatga tggccagggg
    13741 gacccaggca atgggcggga tgggacgcag ggactcgatt atgggatcaa gccaccgttc
    13801 aacggctttc acccctccca ttagaatacc caaagagact cccaggagta aagcggcgcc
    13861 gaagccccag aaaacccggg tcaagcttat gatcagggcc ttgaggaggg cgccggtctg
    13921 ggcaagctcc aggagtcctt tgccagcccc cagcggggta ggcgtcatct ttccgggggt
    13981 gccgaaggta atcgccatct gccagaatag aaagatgagc gccagaagcc aggatttctt
    14041 aatcagtccg acagccatct gcatgaagtg gaactcttct tttcccttag cccagtccgt
    14101 aggatgtttt ccgccaggga atggccttgc ggccgatcca ccggagcaga aggtcgctca
    14161 ggtaacccag gatgccgatg gtggccattc cggcaacgat gctttcgctc atgaacatgg
    14221 aatgggccca ttcgatcttg aagcccaggc cgttgggcgc ccccaccagt tcggcggcga
    14281 tgatggacat ccaggccgag gccatggcca ggcgaccggc cacgatgatc aggggaaggg
    14341 tcccgggaag gatgacatcg cggatgatga gccagcggtt ggcgcccacg gtgagagaaa
    14401 cttcgaaaag cctccggtcg atcgacttga ccccggcaat ggtgttgaga aggaactgga
    14461 aggacgcccc atagaagatg atgtagatcg ccagcccatc tcccaccccg aagagaaaca
    14521 tggcgatggg aatccaagcg atgggggaga tggggcgcaa gacttcaacc accgggctga
    14581 cgatggactc cgcctgccgg gaaaggccca ggagaaaccc gaggggaatt ccaacagcgc
    14641 cagccacgag gtacccgagg atcagccgtt tcagactgat gaaaatatcc aggagaaaat
    14701 gctgctcgga gagaaggtcc cagagcgccc ccgccacctc cggaggagag ggaaggagga
    14761 ggcggttctc gatccagcgg ctcaggagat accagaggat gacgaacaga gtcattgtcc
    14821 ggaagtagga aagggcactc tgggcttttt ctctcagaag gttcatcttt tccccgaatt
    14881 cagcgcgaat tctatgccag cggttctgaa cctgtcaacc ttttcctttg gaggggttcc
    14941 aaattgacaa ttgcgggtaa ttttcctata ttggtctcgc aaattcaatc ccacttcccc
    15001 aaagcctctg caggccatgg aaaggaggag aaccatgaaa aagggcatcc agatgtttat
    15061 tctttcccta gcgataaccg cggggattgg attttccacc ccccgtccca ccttggcgac
    15121 atacccggaa aagcccgtga ctatgatctg cgtcttccct gcggggggag ccatggacat
    15181 gaccgcccgg gccctggcag aggcggctaa gaagtatttc ccaaaaccct ttgctgtcat
    15241 gaaccgcccc ggggccgcgg ggaccattgg gactgcggaa gcgattcagg cccggccgga
    15301 tgggtacacc attggaatca ccgcagttgc ggttttgacc gtgcagccgc accgcaccaa
    15361 acttcccttt ggtccgccgg aagattatac tccgatcatc aagctcgtta accagccggt
    15421 cgtcctggcg gtgaaacagg acgccccctg gaagaccatc caggaattca ttcaatttgg
    15481 caaggccaac ccgggcaagt tgagggtggg atgcccaggg atcggaacga ttcccatgct
    15541 ggatgcggaa cagttcaaga tgatgtccca ggcggatttg accaccgttc ctttcgcggg
    15601 aggggcggag agcgtgccgg ccatgctggg ggggcatgtg gaagcggtct cccagcatcc
    15661 cagtgaggtc atggctcaag tgaaggcggg aaaggccagg gtgcttgtgg tgttcgagga
    15721 aaaacgcaac gccctctttc cggatgcccc tacctctaaa gagctcgggt atgacatcac
    15781 catgggggtt tactacatgc ttatcgggcc caagggcctt tcttctcaga tcatcgccct
    15841 gatccacgac gcagcgaaaa aatcgatgga agaccccatc ttcaccaagc ccatggaagc
    15901 tcggggtttc gacataactt atgaaggccc ccaggatttg aaaaagcgtc tggtgcggga
    15961 ctacgagggg aacgccaaat ttgccgatct cctgaaggaa aagaaatagc cgatgtctct
    16021 ccgagccctg ccgatcgttt ctctctgttt tctcctcttg gggattgcct acctggtcga
    16081 taattccaaa cttcccatgg ggtcggcggc caagccgggg gcagggctgt tccccctgct
    16141 ggtgggcttt gctctttcag gcttaagtct ggccctcttt atctgggctg gaaggaatca
    16201 agacaccgct gcaaaaggcc aggagccctt cccggtggga aaagatggcc agcgggtctt
    16261 ggccctggga gtagtcctgg ccctcttcgt gctcttgctc aaacccttgg gatatggaat
    16321 ctgcggcacg gccctcttgg cggccgttct ccggttgctg ggacttcgga gctggagaag
    16381 gatcattttg atctccctgg tggctgcggc tacctctttt ttcctctttg ataccctttt
    16441 gggggtgcct cttccaagag gggttttctt ctcctgatat ggactctctc cagggattgg
    16501 cctacgggtt cggagtggcc ctgaccggag caaacctcct ggcctgcctt ctgggtgtgc
    16561 tggtgggcac ggttgtgggc gttttgccgg ggatcggtcc catcggagcc atggccctgc
    16621 tgattccttc gaccttcagc ctggggccga ccaccgcttt aattatgctc gcgggaattt
    16681 attacgggtc catgtacgga ggttccacca cttccatcct ggtcaatgtc ccgggcgagg
    16741 ccgcctcagt ggtcaccgcc atcgacgggt accagatggc ccggagagga agggcagggg
    16801 cggctctggc cgtttcggcc gttgggtctt ttgtggccgg gacgctgggg atcgtggcgc
    16861 tcatgctttt tgcccctccc ctggccgagc tggcccttga atttgggcct cccgagtact
    16921 ttgcccttac cctggtgggt ttgttcttcc tttccggcct cggtgggggt tcgaggctca
    16981 aatcctttct tatggtagcc tttggactcc tcctgggaac tgtgggaatg gagcccatct
    17041 ccggcctgcc gcgcttcacc ctgggacagc tctcgctggc gcagggggtg gagctcgtac
    17101 ccgtggccat gggcctttac ggcatcgggg aggtattgat cattgccgag aaaatcaccg
    17161 ggatcccgga actcataaaa gtcaaattcc gcgagctttt tcccacagtg gccgagtgga
    17221 aaagggcttt cccgcccatg cttcggggtg gaggtgtggg tttcctcatt ggactcattc
    17281 ccggaccggc ggcaattctt tccacattcg cttcctacgc actggagaaa agggtctcca
    17341 ggcaccccca ggagtttggg aaaggggcca tcgagggagt ggccgggccc gaggcggcca
    17401 ataacagtgc aacggctggg gccatggttc cccttctggc tttgggtatt ccctttgccc
    17461 cggcgacagc catgcttata ggggctttga tcatccacgg agtccaaccc ggcccccttc
    17521 tcatgaccca gcagccggaa atcttctggg gggtcgtggc cagtatgtac atcggcaacc
    17581 tggtcctttt aattctgaac ctccccctgg tgggtgcctt tgccagtatc ctccggctcc
    17641 cccagcacct ccttatgggg ctcatcctgc tgctctgcct agtgggcacc tacagcgtca
    17701 acaacagcct gttggacctc tacatcctca tcggcatggg aggggtaggc tatttcctgc
    17761 gaaaattgaa attcgacatg gcccccctga tcctggccct ggtcctggga cccatgatgg
    17821 agaagactct ccttcagtct ctgtttatgg cccgggggga tgtatggtcg atcctcagcc
    17881 gtcccatccc ggccgtcctc tattccatcg ccatcgcggt gatggttctc ccccttctct
    17941 atcgatgggt gaaaaagaga tcggaggctc tccgcccatg atggccctct cctcattctt
    18001 gagaagaaga gcaatagcgg attgcgaaac gcccccctcc attttttttc tacccagatg
    18061 gtcaatgttg aagtagaatc agggcgggcc gtcgaaatct atggagaagg tagggataag
    18121 accctgactt catacgggtg agccggcagg cggactagct ccccgcttgc cgagggcctg
    18181 gggaactggg gaaacatctg gtgaagatga tgaggaagtg ggatccttga ttatctagtt
    18241 gggcctgcag gaaaaatgag agtaggggcg actccccggg gggtcgcccc tacggaagct
    18301 gtgagaggga tccatgagcg aaattcgctt cccttccatg agcctgagtg ggaaaatcgc
    18361 catcgtgacc ggcgcagggc aaggcctggg cctgtggatg gcccagggtt tggcccatgc
    18421 aggtgccgat acggcgattg ccgagattaa cccacaggcg ggggaggcag cggccgagga
    18481 ggtccgcaag atcggaagga cggccctatt cattgagacc gacgtccgcc ggggggattc
    18541 catccgggct atgatggacc gtgtagtgaa agaatgggga aggatcgaca tcctggttaa
    18601 caacgccggg gtcaatgtgc ggaaaaaagt ggaggaggtg gagcccgaag atttcgacga
    18661 agtggcgtcg gtaaacctcc ggggtgctta tttctgcgcc caggcagcgg gcaaagtgat
    18721 gattcagcaa aggcaaggca agatcatcaa cattgcctcc gcagccgggt ttctggtccg
    18781 gtccgggatt cccaattcgg tctactcgat caccaaggcg ggagtgatca tgatgaccaa
    18841 ggccctagcc gcggagtggt ctccctacaa cgtcaacgtc aacgcggtcg cccccgggta
    18901 ttttgtcact cccctcacgg ttccgcggtt caaggaccct aaggcctggc aggccatcat
    18961 ggactcgact ccactaaaac gagtcggaca agcggaagat atcatcgggc ccgtggtttt
    19021 cctggcttcc gatgcggccg cgttcatcac cggggcatgt ctctttgtag atgggggaag
    19081 aacggtattg tgaatggtcc tatgatcaat gggcaatgtt cactgcaaag agttcttatt
    19141 ggcaggcatg aggtcggtgc aggaggctgg ggaacctcca tttacggtgc aattcgcggg
    19201 tgccggacag ccttgagacc gagatagctc agcgttgaaa tcctcaatcg gaaaggctat
    19261 ccagggccct cccttcggca ttttgccttg cactatacgg agtgcagggc agacttgaac
    19321 tttacatggt tcaaggtgaa ttcccccttc tgcattggaa agggtcagga ctgaccactc
    19381 ggaccaaata tttcgtcgtc gccctcctct atttctccga agggcttccc ttcgggctga
    19441 tcaacaacct cctttcggtc tacttccggg taaagagaat ttccctggag gagatcggtc
    19501 tgctcagcct cctgggcttc gcctggagcg taaaaatcct atgggccccc ctggtggacc
    19561 gttttgggag gaggtatttc tggattctcc ccgctcagct gctcctcgct tgcttcagcg
    19621 cagtcttggc cttcatcgac ccggtctccc agaaggccct gttctggctg gcgctcgccg
    19681 gggtctgcct ggcctccgcc acccaggaca ttgccgccga cgcttactct atcgacatcc
    19741 tggaggagaa agagctcggt ccggccaacg gggtccgctc cgccgcctat cgagtggcgc
    19801 tgattaccgc cggcggagtc ctagtcatgc tgagtgaaac catgggatgg tccctgacct
    19861 tcatcgccgc cggggtcatt atggcggttc tcgctctggc cgtggttagc tttccttcct
    19921 tccgccagaa aaggccggtg gtttctgcct ccctccccct atgggagcaa tacgtggacc
    19981 ccatcaagag gctcttcctg aaacctaact tctggaccgc cgtgatcttc atcctttttt
    20041 tcaaagtagg ggaagccatg ctagtggcca tggccaatcc cttctggatt gatcagggct
    20101 tcactccggg agagattggt tttgcggtgg gcaccctggg aaccctggca agcatcgtcg
    20161 gagccatcgg tggaggggga ttgacggccc gatgggggat ccggaaagct ctctggattc
    20221 tgggggcagt gcaggccaca gccagcctgg ggtacgcttt cgcctctctg cctctggccc
    20281 cgcgcttctc catctacttc gccgcccttc tggagagcct ggccattggc ctggccaccg
    20341 cggccttcct tagctttctt atgaaactct gcgaaaaaaa gttcagcgct acccattacg
    20401 cttttctcac gatccttttc ggtctgggcc ggtccctggc cggcgtgctt ggaggctatt
    20461 cggcttcatt ccttggctac tcagtctttt tctttgttac gtttattatt ggcctcgccc
    20521 ctttgccgct gatcctttat ctccaaccaa cgttaaacaa gggttaatga tcaatggaca
    20581 acgccaaaag gataataaac aatcaacaga ggaacgagga acaaattggt taggagagac
    20641 ctcagagata agatcgccca gatgatcatg gtgggattcc gggaggcggt ggttgacaaa
    20701 gatacccccg tcgttcgggc aataagagat catggccttg gcggtatcat cctctacaac
    20761 atcgacctga agagctttct cgaggcgcag aagaaaaaac cggacctgga ccgcacggag
    20821 ggagcaaagc tctgcccaaa gaatatcctc tccagaggcc agctgaaaac tttgacctcg
    20881 gccttgaaga gttactcgaa gacgccgctg ctgatctccg ccgaccagga agggggaatg
    20941 gtcagccgcc tcgggccggc tgccggattt gcggaaaggc tgagccctcg agccttgggg
    21001 gagaaggatg acccgccggc aacggtgaaa gaagcggagg cgatggccca agacctgtgg
    21061 gagaccggaa tcaacctgaa cctggcgccg gtggtcgacc tcaatctgaa tccggaaggc
    21121 ttgatcgcca gaatgggacg atcctttggg gccgatccag acctggtttg ccgccacgcc
    21181 gaagttttta ttcaagccca ccgggaaagg gggattctta ccaccctcaa acacttcccc
    21241 ggaaaaggaa gcgtagggaa agacactcac ttcgaactgg ccgacgtaac ctccctctac
    21301 caggatcagg agctccagcc attttcccgg ctcatccggg aaggaagggc cgacctggtc
    21361 atgacttccc acatcaacca tcgaagatgg gatgaagatt atccggtcac cctctccccg
    21421 agaatcctcc aagggctact tagggaaaaa ttgggctata ggggggttat catctccgac
    21481 gacctgctca tgggggccat tatcaagcag ttcagcctgg aggaggcctg cgtgctggcc
    21541 gtgcaggccg gggtggacat tctcctggcc tccaacaacg ccccggaagg ggataatccc
    21601 gacctttttt cccatatctt cgaggctctc cttcgggctg tggaggaagg ccgaatcgcc
    21661 ccccatctca tcgaatcatc caactctcgc atcctgacgg tgaagaaaag tcttcccccc
    21721 tcctccctct ccgcctgagt ctttcccctc aagggaggcc ggaattttag ccgagaaagt
    21781 gcggggctaa aagaacggcc gtttaagaag ggtggagccg gaccctagcc caactttcgt
    21841 caaagggcgg ccagctcttc atcccgaatg tcggaaatga acatgtgccc ggcggaatga
    21901 gtaatcatga tctccggttt tgaagccatg gccaccgcct gaggggtcac tccacaggcc
    21961 caaaacacag gaacatcatc cttctcgaag cggaggggat ctccccagtc cactttttgg
    22021 aggtcctgga tcccgatttt ctttgggtct ccgatgtgta ccggggcgcc atgagtcgcc
    22081 gggaatcggg aagtaacctg aatcgcgcgc accaccaatt tttcgggaat cggacgcatg
    22141 gaaacgacca tgggcccgga aaagaccccc gccggagcgc agggaatatt cgtgatgtat
    22201 acggaaacat ccttcttatc ctccacatgc cgcaagggaa tgttggcctt ggccatggcc
    22261 gattcgaagg tataactgca gccgatgaga aaagccacca ggtcatctcg ccaataatct
    22321 tttatggttt ccacctctct caggtatttt cctttttcgt agatgcggta cttggggatt
    22381 tctgtgcgga tgtccgctcc cttggcaatc tgggaaagaa tggggttccc cggttccgtc
    22441 acttccagaa cgggacaggg tttggggttc ctttggcaaa aaagcaggaa ttcaaaagca
    22501 aggttccggg gaagaatggc caggttggcc tgggcatatc ccgcggaaac aaaggcggtt
    22561 cccttccgcc attttccctc gcggatgagt tgacggactt ccctgggggg caaatcccgc
    22621 aatttttctc ggtccatcgg tttttccttt cttttccatt aagggtatgg gaattctttt
    22681 tcagattggt tcgaagcgta cgggatgaac cgcccgtcgg tttcctaccg cttcttcaat
    22741 agccccatta acccggtagg ttgaaaccga gctatgagaa tgatgaggaa accataaatc
    22801 atcaagtcaa ccgccgttcc cctgcctccc agataggtcc gggtgaattc ggaaagggga
    22861 atcagcactg ccgctcccag gaagggcccc cacaccgtgg ccacaccacc gaggatggcc
    22921 accaagctaa tcaacacaga aagcattagg gggaggacgc tttccggatc gataaacagg
    22981 acgtactggg aatagaaagt cccggcgata gaagtaaaga atgcgctgat acccaaggcc
    23041 acagtcttgt agcgcgcgac atggatgccc actgcgcggg aagcatccag gtctcctttg
    23101 atggcccgaa aataataacc caaaaaggac ctctccagga agaaaaccgt ggcaaaggtg
    23161 atcgccgcca tgcccaaagc gatatagtaa tagggaacct tggtgttatg gaattccaga
    23221 ttgagaaaac cctcttccag aatgggcaaa tagagtccgt tcgctccccc tccgcccttc
    23281 caattgacca taccgatctg acagatttcc ccgatggcga tggtggcaat ggcaaagtaa
    23341 tgaccggcga ggcggaagca aggaatggcc acgaggagcg agaggcccac ggccagaatc
    23401 gctccgacca gcatccccag
//
LOCUS       DASPRR010000640         6038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_305498, whole genome shotgun sequence.
ACCESSION   DASPRR010000640 DASPRR010000000
VERSION     DASPRR010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305498"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VEL68_23485"
     CDS             <1..342
                     /locus_tag="VEL68_23485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXZ39011.1"
                     /translation="RPRFYRQPAGFRNPKIFGILKEMGMSLVGWQTHAFDTVTRSPGG
                     IAQRILKTFQAGGIILLHDGWDRDGEKDRTPTLEALPFILQGLKDRGLQFFTLDRLLG
                     MSKEAVKNNDQ"
     gene            420..1112
                     /locus_tag="VEL68_23490"
     CDS             420..1112
                     /locus_tag="VEL68_23490"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXZ39012.1"
                     /translation="MKIMIRILFLSISAIALQASLYGSLSFAQDAQELIRVIDEQQKK
                     VQTLVAGFSQKKETSLAKDPLLSSGMVKFKRPDRVHFIYSQPEQMEMALEGKTIWIYY
                     PIQSQAEKYSFARNKRISQYLEPVTAIFQKTFGQLAETYDIVYQGEESDRLYRFRLQP
                     KEEKTQKFLSRVNVWIDKGSGAILRFEMIEASKDRLWMEFKDLQINPPLKDEDLTIRI
                     PPSVRVLEHTPP"
     gene            1109..2329
                     /locus_tag="VEL68_23495"
     CDS             1109..2329
                     /locus_tag="VEL68_23495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXZ39013.1"
                     /translation="MNRRRAVITGLGVLTSIGQDTPSFWKSLVEGVCGIDRITLFDPS
                     GYRVQRAAEVKGFDPGRYFPPRRLKRMSRCDQLGMKAVREALKDSGLDLEKEDRQRMG
                     IFIGGGAGGILSAEKYRKEMIRKGWRRARPSLLLPFSTCALNDAIAEEYQVLGPRATV
                     ATACSSSATAIGCGLDGIRSGDIDAAIAGGSESLSEVTFGGFNSLRSVDEEYCRPFDL
                     NRKGLSLGEGAAFLILEEEEHARKRGARIHAVLMGYGLSGDGQHMTAPDPAGKGAARA
                     MQQALKDAGQSPDGVGYINAHGTATLANDAAETKAIKSVFGKRAGKIPVSANKSMIGH
                     CLGAAGALEAVATVLAVRDDRIPPTIHYQTPDPDCDLDYVPNQARDAAVGIALSNSFA
                     FGGNNTALVFGKWK"
     gene            2618..3835
                     /locus_tag="VEL68_23500"
     CDS             2618..3835
                     /locus_tag="VEL68_23500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXZ39014.1"
                     /translation="MEEIVVTGIGIISACGIGKKAFWSSCLEGRTGIAPIRSFDTSGY
                     PSHLGAEARDFNPKDFMPPLKYRRMSRVSRLAVAASIEAIQDAALEVSPETALSIGVV
                     LGTGYGSTAQTDDFFVGMLKEGPEGANPSLFPDTVPNAPASQVSIYSRLQGPNTTFSH
                     NEVSGEQAMAYAFRLLQEDRAQAVLAGGVDEISFVLFHSFSALRALSPRDEQGEKMRP
                     FDRKRNGRILGEGAGILVLEKKTRAKERGARIYGSLVACASTGSPAGISRYEAGAEQM
                     ARAMENTLQQAHISPDEVDYVSAAANSSLELDRAEAKAIQRSFGGGSSLPAVSSLKGH
                     TGDFCSSGALRAAAILLAMSDRKIPPTMGLKDPEFDLDHVRSIPREAKIQYALLNGFS
                     FGGSNVCLLFKNE"
     gene            3998..5281
                     /locus_tag="VEL68_23505"
     CDS             3998..5281
                     /locus_tag="VEL68_23505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXZ39015.1"
                     /translation="MKIKIICPRWPAKSLWRHFIFRFPYLSLTTLAALTPREFEVVIE
                     DENVQEVSFDDRPDLVAISIMTPLAKRGYAIADRFREESVPVVIGGFHATWLSEEAGQ
                     HADSVVLGEAEQSWARLLEDFKAGCLQKFYRAEGKADLKGLPFPRRDLLKKRGYFFTN
                     TIQVSRGCPFQCSFCSVTAFFGHTFRMRPLDEVKKEVEILLQGQDFIFFVDDNIIGNV
                     DYARDLFSFLKGLKVKWVSHSSINIAENEDLLTSAAESGCHGLFIGFESLSQDTLKSH
                     RKTSNQVERYKEQIRKIHDRGIGIEGSFIFGSDEENSSVFPRVVDFCEETKIDAAVFA
                     VLTPYPGTRIYEQFVKEDRILSWDWDLYDMDHVVFRPRSMTVEQLQEGHDWASRRFYS
                     YPSMLKRFWPLRRSHQVFLPSNWGMRRAWKHMMKS"
     gene            5355..6035
                     /locus_tag="VEL68_23510"
     CDS             5355..6035
                     /locus_tag="VEL68_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent methionine synthase"
                     /protein_id="HXZ39016.1"
                     /translation="MESTILAALPFKVDLEQLKKNLRVKEGTEMVGKLEGMVQEAQKI
                     AKPKVCYRIAYVESKGDDHLMIEGIQFTSRVLRVNLDKVYRIFPYVATCGLELEEWSK
                     SVDGILEQFWAEEIKVLAVRGTIRYLQEYLTDRYQPGQMSRMNPGSLPDWPLSEQKPL
                     FALLGNGPAQIGVQLRDSFLMIPVKSVSGIWFPTEEKFESCQLCQREKCPGRRAPFEP
                     NLFDRKYR"
BASE COUNT         1406 a         1594 c         1730 g         1308 t
ORIGIN      
        1 cggcctcgct tctaccgcca gcccgccggg tttcgaaacc ccaaaatctt tggtatctta
       61 aaagagatgg gaatgtccct agtaggatgg cagacgcacg cctttgacac cgtgacgaga
      121 agccccggag ggatcgccca aagaatcctg aaaaccttcc aggcaggagg gatcatcctt
      181 ctccatgatg gctgggaccg ggatggagaa aaggaccgaa ctcctaccct ggaggccctt
      241 cccttcattc ttcaagggtt gaaggatcgg gggctccagt tcttcaccct ggacaggctt
      301 ttggggatga gcaaagaagc ggttaagaac aatgatcaat gatcaatggc caatggtcga
      361 tgatcaatgg gcaatgaaga cattgcctcg tggcaattga attctgaaat gacggcaaga
      421 tgaaaataat gattcggatt ttattcctct cgatatcggc catcgccttg caagccagcc
      481 tgtatggctc tttgtccttc gcccaggatg cgcaggagct cattcgagtt atcgatgaac
      541 agcagaagaa ggtccagacc ctcgtggccg gtttttccca aaagaaagag acctctctgg
      601 ccaaagaccc cctgctgtcc tccgggatgg tcaagttcaa gcggccggac cgggtccatt
      661 ttatctattc ccagcctgaa cagatggaaa tggccttaga gggcaagacg atttggattt
      721 actatcctat tcaatctcaa gcggagaaat attcctttgc ccggaacaaa aggatcagtc
      781 aatacctgga gccagtgacc gcaatctttc aaaagacatt tggccagctc gctgagacct
      841 acgacatcgt ctaccaaggc gaagagtccg atcgccttta tcgcttccgc ctgcagccaa
      901 aggaagaaaa gacccaaaag ttcctttccc gggtgaatgt ttggattgac aagggttccg
      961 gggccatttt aagatttgaa atgatcgagg ccagcaaaga tcgcttatgg atggaattca
     1021 aggacctgca aatcaacccg ccgctgaagg atgaagactt aaccatccga atccctccct
     1081 cggtcagggt attggaacac accccgccat gaaccgccgc cgcgccgtca ttaccggatt
     1141 aggcgtcctc acctctatcg gccaggatac cccctctttc tggaagagcc tggtggaagg
     1201 ggtttgcggg atcgatcgga tcaccctttt tgatccttcc ggctaccgag tgcagagggc
     1261 cgcagaagta aaaggatttg atcccggccg gtactttcct ccccgtcgac tgaaaaggat
     1321 gtcacgctgc gaccagctgg ggatgaaagc ggttcgggaa gccttgaagg actccgggct
     1381 ggacctggaa aaggaagacc ggcagcggat gggaatcttc atcggcggcg gggccggggg
     1441 aattttatcg gccgagaaat accggaaaga gatgatccgg aaaggatggc gcagggcgag
     1501 gccgagcctc ctccttcctt tttccacctg cgccctcaat gacgccattg ccgaagagta
     1561 ccaggttctt ggaccccggg cgacggttgc taccgcatgt tcatcctcgg ccacggccat
     1621 cggctgcggc ctggatggta tccggtccgg ggacattgat gcggcaatcg cgggggggag
     1681 tgaatccctg tccgaagtca ctttcggcgg gttcaacagc ctgcgctcgg tggatgagga
     1741 atactgccgg ccctttgacc tcaaccgaaa aggactttcc ctgggagagg gggcggcttt
     1801 tttgatcctg gaggaggagg aacatgcccg caaacgcggg gcccgaattc atgccgtgct
     1861 catggggtat ggcttaagcg gagatgggca gcacatgacc gctccggacc cggcgggaaa
     1921 aggcgctgcc cgggcgatgc agcaggcgct gaaagacgcg ggccagagcc cggatggcgt
     1981 ggggtacatc aatgcccacg ggaccgcgac cctggccaac gatgcagccg agacgaaagc
     2041 cataaagagt gtgtttggga agcgcgcagg gaagattccg gtgagcgcca acaaatccat
     2101 gattgggcat tgcctcgggg ctgcgggggc tttggaagcc gtggcgaccg ttttggcggt
     2161 gagagacgat cgaattcccc ccaccatcca ttatcagacc ccggacccgg actgcgacct
     2221 ggactacgtt cccaaccaag cacgggatgc ggcggtgggc atcgcccttt ccaattcctt
     2281 cgctttcggg ggcaacaaca cggccctggt ttttggaaaa tggaagtaaa ggcagctatc
     2341 agtgctcagc gatgagcgaa gacgcaaata agaatccacg aggattgatg aactcgtaaa
     2401 aaggtgaaat tccataaaat tcgtcattcc ggcgaaagcc ggaatccagg gttcctcgag
     2461 aaaactggga tccagtcttt gagaggattc ctgactttcg tcggtacgac gtctggaccc
     2521 cggctttcgc cggggtgacg cttgatgaga ctttttacga aatcacaaag attctttggt
     2581 ggaagtctga tagctgaccc ctgaaagctt ttcctttatg gaagaaatcg ttgtcaccgg
     2641 catcggtatt atctctgcct gcggcatcgg gaaaaaggcc ttctggagca gctgtctgga
     2701 agggcgaacc gggatcgctc ccatccggtc cttcgatacc tccggttatc cttcccacct
     2761 tggagctgaa gcccgggact tcaaccccaa ggacttcatg cctccattga aataccgacg
     2821 gatgagccgg gtctcccgcc tggcagtcgc cgcgagtatt gaagcgattc aggacgcagc
     2881 tctggaagtc tcccctgaaa cggccctgtc gatcggagtg gtcctgggga ccgggtatgg
     2941 aagcactgct cagactgacg attttttcgt aggcatgctc aaggagggtc cggagggggc
     3001 caacccaagc cttttccctg ataccgtgcc gaatgcccca gcaagccagg tctccatcta
     3061 tagcaggctc caagggccca atactacatt cagtcacaat gaagtttccg gggaacaagc
     3121 catggcctat gcctttcggc ttctgcaaga agatcgtgcc caggctgtgc tggcaggagg
     3181 ggtggatgaa ataagctttg ttctcttcca cagcttttcc gccctccgcg ccctttcccc
     3241 ccgggatgaa cagggagaga aaatgcgtcc ctttgaccgg aagaggaatg gccggattct
     3301 gggggagggg gctgggatcc ttgttctgga gaagaaaacc cgggcgaaag agaggggggc
     3361 tcgcatttac ggctccctgg ttgcgtgtgc ttctaccggc agtccggcgg gaatttcccg
     3421 gtatgaagcc ggggccgagc aaatggcccg ggccatggaa aatactctgc aacaagccca
     3481 catttctccc gatgaggtcg attacgtctc cgccgcggca aattcgagcc ttgagttgga
     3541 cagggcggaa gccaaggcaa ttcagagaag ttttggtgga ggaagttctt taccggcggt
     3601 gagttccctg aaaggacata cgggggactt ttgcagctcc ggggctctgc gcgccgcagc
     3661 gatccttctg gccatgagcg atagaaagat tcctcccacg atggggctaa aagacccgga
     3721 atttgatctg gatcatgttc gcagtatacc ccgggaggcg aaaattcaat atgccctcct
     3781 gaacgggttc tccttcggcg ggagcaacgt ttgtttgttg ttcaagaacg aataatactc
     3841 aaggcagatg aacgcagatt ttcaggataa gcattatata atgaaggtcc ttattgacag
     3901 tcaacgattt tttctctgct gaaaatagag attttgttga cttttgtaaa tacggatttt
     3961 tatgtgccta tccttgttaa tctgtgtccc ctaaaaaatg aaaattaaga ttatctgtcc
     4021 ccgatggccg gcgaaaagcc tctggcgcca tttcatcttt cgatttccct acctgagcct
     4081 gacgaccctg gcggccctga ccccccgaga atttgaggtt gtcatcgagg acgagaacgt
     4141 ccaggaagtc agcttcgacg accgccccga cctggtggcg ataagtatca tgacccctct
     4201 ggcgaaacgg gggtatgcca tcgccgatcg tttccgagaa gagtctgtgc ccgtggtcat
     4261 aggcggcttc catgccacct ggctgtccga agaggccggg cagcatgccg actccgtggt
     4321 tttaggcgaa gcagagcaat cttgggctag gcttctggag gattttaaag cgggttgcct
     4381 tcaaaagttc taccgggccg aggggaaggc ggacttgaag ggacttccct tcccgaggcg
     4441 ggatctgctg aaaaaaagag gctacttttt caccaacacc atacaggtca gccggggatg
     4501 ccccttccaa tgcagtttct gctcggtcac cgcattcttc ggccatacct tccggatgcg
     4561 ccctctggat gaggtcaaga aagaagtgga aatcctgctg caggggcaag acttcatctt
     4621 tttcgtcgac gataatatta tcggcaacgt ggattacgcg agggaccttt tttccttttt
     4681 gaaaggcctg aaggtcaagt gggtgagtca ttcctcgatc aacatcgccg agaatgaaga
     4741 tttgttgacg agcgcggcgg agagcggctg tcacggtctg ttcatcggct ttgaatccct
     4801 ttcccaggat accctgaaga gccatcgcaa gacttccaac caggtggaac ggtacaagga
     4861 gcaaatccgg aagatccacg accgaggaat cgggatcgag gggtccttca tcttcggttc
     4921 ggatgaggaa aattcttccg ttttcccccg ggtggtggac ttctgcgaag agaccaagat
     4981 cgatgcggcg gtgttcgccg tcctgacccc ttaccccggg acgcgcattt atgaacaatt
     5041 tgtgaaagag gacagaatcc tttcctggga ctgggacctc tatgatatgg accacgtcgt
     5101 attccgcccc aggagcatga ccgtggagca actccaggaa ggacacgact gggcgagccg
     5161 ccgtttctat tcctacccct ccatgctgaa acggttttgg ccgctccgca ggagccacca
     5221 ggtctttctg cccagtaact ggggaatgcg ccgggcctgg aagcatatga tgaaatctta
     5281 aattcccccc accccttcct tcttcctcga ggggaggata ggaggggggt gatgcgttaa
     5341 aggaggctga accgatggaa tcaactattt tggcagccct tcccttcaag gtcgatctgg
     5401 aacagttgaa gaagaacctg agggttaagg aaggaaccga aatggtcggc aagcttgagg
     5461 gcatggtcca ggaagcccag aagattgcca agccgaaagt ctgttaccgg atcgcctacg
     5521 tggaatccaa aggcgatgac catctgatga tcgaagggat tcaattcacc agccgggtcc
     5581 tccgggtgaa cctggacaag gtgtaccgga ttttccccta tgtggcgacc tgcggactgg
     5641 aattggaaga atggtccaaa tccgtggatg gcatcctgga acagttctgg gccgaagaga
     5701 tcaaggtgct tgccgttcgc gggacgatcc ggtatttgca ggaatacctg acggatcggt
     5761 atcagccggg gcagatgtcc cggatgaacc ccggatcact tccagactgg cccttgtcgg
     5821 aacaaaagcc gctttttgcc cttctgggaa acggtccagc ccaaatcggg gtccagctaa
     5881 gggacagttt cctgatgatc ccggtcaaat ccgtttcagg gatctggttc cccacggaag
     5941 aaaagttcga gagctgccag ctctgccagc gggaaaaatg ccccggccgc cgcgcgccct
     6001 tcgaaccgaa tctgtttgac cggaaatacc ggtaaaag
//
LOCUS       DASPRR010000641         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1287308, whole genome shotgun sequence.
ACCESSION   DASPRR010000641 DASPRR010000000
VERSION     DASPRR010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287308"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VEL68_23515"
     CDS             <1..1600
                     /locus_tag="VEL68_23515"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HXZ39017.1"
                     /translation="QYFFVSCSLQDMIRIHRLKELPLNRLHETFAVQLNDTHPSIAVA
                     ELMRLLVDEHFLDWEEAWAITRKTFAYTNHTLLPEALEKWPLPLFREMLPRHLEIIYE
                     INRRFLEEVRQKYPGDDRKAARLSIIDETHDKYVRMAHLASVGSHAINGVAALHTELL
                     KQTVLRDFYELYPDNFHNVTNGVTPRRWVALSNPRLAELITRRIGDRWVRHAEDLRKL
                     ELFADEPEFQKEWQRVKLENKRRLAGLIKDQTNIVVDPGSLFDILVKRLHEYKRQHLC
                     ALHAITLYNRLKKNPKEDIPPRTVIFAGKAAPGYFMAKLIIKLINSAAEVINKDPDVA
                     DRLKVIFFPNFNVKNALPIYPAADLSEQISTAGKEASGTGNMKFSMNGALTIGTLDGA
                     NVEIREEVGAENFFLFGLTAREVANLKSKGYNPGEYFHSNPELREAIDQVRSGFFSKG
                     DKNLFLPLVDFLISRDEYLLFADYASYLDCQERVGRAYQDQKNWTKMSILNVARMGKF
                     SSDRSIREYAEKIWKAKALGAVEE"
     gene            1854..>2251
                     /locus_tag="VEL68_23520"
     CDS             1854..>2251
                     /locus_tag="VEL68_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HXZ39018.1"
                     /translation="MATSERSGETRRNGVSRIFGGRSFPLGATVYPDGINFSVFSRGA
                     DAIELLLFDRNDDAEASRIIPLDPKKNRTYHYWHVFVPNLGPGEIYGYRANGPHDPSK
                     GLLYDPEKLLLDPYGRAVTVPDCYRRAAAS"
BASE COUNT          521 a          627 c          626 g          477 t
ORIGIN      
        1 gcaatacttc ttcgtctcct gctcccttca ggacatgatc cgaattcacc gcctgaaaga
       61 gcttcccctg aaccggctcc acgagacctt tgccgtgcag ttaaacgaca cccacccatc
      121 catcgccgtg gccgaactga tgaggctgct ggtcgacgag catttcctag actgggagga
      181 agcctgggcg atcacccgga aaactttcgc ctataccaat cacacccttc ttccggaggc
      241 cctggagaaa tggcccctgc ctctctttcg ggaaatgctg cctaggcatc tggaaatcat
      301 ttatgaaata aatcggcggt tcttggagga ggtccgacag aaatatccgg gggacgatcg
      361 gaaagcggcc cgcctctcca ttatcgacga aactcacgat aagtacgtgc ggatggctca
      421 cctggccagc gtgggaagtc acgccatcaa cggggttgcc gccctgcaca ccgaattgct
      481 caaacagacg gtcctgcgcg atttctatga gctctatcca gacaattttc acaacgttac
      541 gaatggcgtc accccgcggc ggtgggtcgc cttgagtaac ccgaggctgg ccgaattgat
      601 cacccggagg attggggatc gttgggtccg ccatgccgaa gacctgagga aactcgaact
      661 ctttgccgac gaaccggaat tccagaagga gtggcaacgg gtcaaactgg agaataaacg
      721 caggctcgcc ggcctcatta aagatcaaac caatattgtc gtcgatcccg gttcgctttt
      781 cgacatcctg gtcaagcggc tccacgagta taaacgccag catctgtgcg ccctccacgc
      841 catcaccctg tataaccgcc taaagaaaaa ccccaaagag gacatccccc cacgcacggt
      901 catctttgcg gggaaagcgg ctcccggata cttcatggcc aagttgatca tcaagctgat
      961 caactcggcg gcggaggtga tcaacaaaga cccggatgtg gcggaccgcc tgaaggtgat
     1021 cttctttccc aacttcaatg taaaaaacgc cctgcccatc tatccggctg ccgatctttc
     1081 cgagcagatt tccaccgccg gaaaagaggc ctcggggacg gggaacatga aattttccat
     1141 gaacggagcc ctgacgatcg ggaccctcga cggtgcgaat gttgagatcc gcgaggaggt
     1201 gggggcggaa aacttcttcc tcttcggctt gacggccagg gaggtcgcga acctgaaatc
     1261 gaaaggctac aaccccgggg aatacttcca ttccaaccct gagcttcggg aagccatcga
     1321 ccaggtccgt tcggggttct tctccaaggg ggacaaaaac cttttcctcc ccctggtaga
     1381 tttcctgatc tcccgcgatg aatacctgct ttttgcagac tatgcttctt acctcgattg
     1441 ccaggaacgg gtaggccggg cctatcagga tcaaaagaac tggacgaaga tgtcgatctt
     1501 gaacgtcgcc cgtatgggaa agttttcttc agaccggtcg atccgggaat atgcggaaaa
     1561 gatttggaag gcgaaggcgc tcggtgcggt tgaggagtga tatgggggag gagaactttc
     1621 ttgactggcg gggatatcga tactcaatct ccccctcatc ctaccctctc ccgcgagggg
     1681 agagggtaaa ctttttgaaa tagaatagaa tttccctccc ccttgatggg ggagggcgag
     1741 ggtgggggtg tttcagggga ttttttgaga gcttcagagg gttgaaaggg gatttctggc
     1801 agggctgtgg tgctgaagaa atctttggct accggagatg ggggcctttg agaatggcaa
     1861 cttcggagcg atccggcgag actaggcgca acggcgtgtc ccgaatcttc ggcgggagaa
     1921 gttttcccct cggggccacg gtctatcccg atgggataaa cttcagcgtc ttttcaaggg
     1981 gcgctgacgc catcgaattg ctccttttcg ataggaatga tgatgcagaa gcgtcgcgaa
     2041 tcattccgct cgacccgaag aagaaccgga cctaccacta ctggcatgtc tttgttccca
     2101 acctcggacc aggcgagatt tacgggtacc gggcgaacgg accgcacgat ccttccaaag
     2161 gccttctcta cgatcccgaa aagttgcttc tcgaccctta tggccgggca gtaacggtcc
     2221 ccgactgcta ccgacgagcg gcggcgagcc a
//
LOCUS       DASPRR010000642         9853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1933833, whole genome shotgun sequence.
ACCESSION   DASPRR010000642 DASPRR010000000
VERSION     DASPRR010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933833"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1530)
                     /locus_tag="VEL68_23525"
     CDS             complement(70..1530)
                     /locus_tag="VEL68_23525"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXZ39019.1"
                     /translation="MNLVHFLLTLKNMSYFQSSLGIDFKANYLILTLMRRSFRKVRLV
                     DYRVFPLGWDGQGENQEAKWISFIRTFIFKNRGGQERISVSIPRETTLVRFLRLPLAA
                     KENLAKVLEYEAPKLTPFDKDEFYSDFQVLKEDSQWIYLIAVFARKEDLAPYLNLLKR
                     MGIQPISIQISAISALNLFFYHEEQKKNEVSVLLDVNDPNFEMNILEGADWKDSFHLP
                     LPKEEKEEGVLRAFKRSELNDLSLSKGMFFVYGLDAIENTLPQFREAHGMKGVASPPM
                     GRIEVGPDETLPDYIYPSLGLPLTGLMQTPFRLNLLPPEMRKKIPQYGKKAFLILLCM
                     AIILGLTWGGGIYSRYRNELEVLRAEVKKKKPEADAVEKLQKQRTKEAAEIAEFDKII
                     RGAISEVEILRELSQILPSSVWVWQYKFAGKEIEISGFADSASELIPLLDKSPIFEKV
                     EFMAPVTKERERRLGADKERERFKIKMRIEGLGGTP"
     gene            complement(1562..2584)
                     /locus_tag="VEL68_23530"
     CDS             complement(1562..2584)
                     /locus_tag="VEL68_23530"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39020.1"
                     /translation="MSPKLAASRGVALVMVLWIITILSVVVLEFSFAMRTEVNITRNY
                     HEQLKLYAAAQGSLQRAVAELILKQDPRFQQLKKNVKPGETPIEYNEWVTDGREYPVA
                     FEGVEGAVRVMGEAGKVNINRVSDGMLRKIMANLGLEMDARDIVVDSILDWRDPDDFV
                     RVNGAENEYYQSLKEPYDCKNGDFDSAEELMLVRGVTPELFYGQKAKGREGEVPKEEP
                     IGLKDIFSLYALGEQIDINSASLPVLRVVLSLPVEVCRQIIKAREDKGFENFPDLLQR
                     VPELSPFSVEVQKLILFAATNPYYTVEARGKTKEGGAVRGIRAVVKIDSREQDGYKII
                     QWLDFL"
     gene            complement(2627..3391)
                     /locus_tag="VEL68_23535"
     CDS             complement(2627..3391)
                     /locus_tag="VEL68_23535"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXZ39021.1"
                     /translation="MGKRPRTGPWPRGWTAAFTLVEVMVTMTILGFLLLMIFGTFRLG
                     LSAWEKGESTKEEYQRARIVSQLIFQQTKSAIAYKVKTQKAEGDFLAFEGRARSLKFV
                     STVSLKARKPSGLVYAFYEFREGGRGGGQLILYEGRVLNKNFLDEPPGEEAAIALLEG
                     LGDVRFEYYQEEDTNKNKTEGWVEEWSAKEENALPTAVRVTVFPLKGAGWTEEAPLKI
                     LASLPANRYEDIRSQGVSRGPVSASTGPPTRGRVGP"
     gene            complement(3375..3800)
                     /locus_tag="VEL68_23540"
     CDS             complement(3375..3800)
                     /locus_tag="VEL68_23540"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXZ39022.1"
                     /translation="MRSRRKKAPGFTLIEVVVAMAILSIGLVVIIELFGGGLRLGRVS
                     EEYTKAAGYARIKIEEISLDKSLAEGVYQGEFDSNYRWQVEVKRVDLIPPGKDITYRP
                     SVALYRVRIDVLWRSGLKDRVASIESYRLLKEEESGQKT"
     gene            complement(3797..4312)
                     /locus_tag="VEL68_23545"
     CDS             complement(3797..4312)
                     /locus_tag="VEL68_23545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXZ39023.1"
                     /translation="MRNSGFSLIEMVIVLVLVSLSIALVTPSLSRFSKGVELKATAQR
                     VSGILRYFRSESIQKGRVYQILFDSGTGEIRIRSLEAEEKEGERKQEEGKKGTEEAPA
                     QERRYPLPGGILIKEVRIPSPEFPSDLPTIEFYPTGGSNGGAVLLEMQDQKGYWIKVH
                     FLTGIVGIEGT"
     gene            complement(4309..4740)
                     /gene="gspG"
                     /locus_tag="VEL68_23550"
     CDS             complement(4309..4740)
                     /gene="gspG"
                     /locus_tag="VEL68_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXZ39024.1"
                     /translation="MAAGHKQRGFTLVEILVVIIIIGLLAALVGPRLFGKVGMAKLKA
                     AKAQIELFGTALDALRLDVGRYPTSEEGLKALREKPSAMDLWKGPYLPKEIPLDPWGR
                     PYIYKCPGEQGDYDLISYGLDGVPGGEGENEDVVSWKDVKG"
     gene            complement(4996..6207)
                     /locus_tag="VEL68_23555"
     CDS             complement(4996..6207)
                     /locus_tag="VEL68_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXZ39025.1"
                     /translation="MAEFVYKATKTSGEIVEGSMEGRDEKAVVHSLHELGYIPVRVIL
                     AGESGASPRFSFLPQRVGLKHVLALSQELSTLVTAGLPIDRSLQILGSLAKNPKLKGV
                     VQDILNRVREGSSLADALGNHPRIFSKLYVNMVKAGESGGFLEVILSRLVKYLQSAKE
                     LRDFLVSVMIYPLILTAVSGASIVILVTFVIPRFAKIFSDMGQAIPLPTQVLLTLSQV
                     FRDYWWLGAGMLALIYFFLRAYNQEEERRFRWDRSKLRWPAVGNIIKKIEVARFSRTL
                     ATLLQSGVPILSALNLVKEISQNLAVSRAIANIHDRLREGKAVARTLEETGVFPPLAV
                     HMISVGEETGRMEEMLSKVAETYEADVQTTVKRFVSLLEPLIILFMGAVVGFIVISML
                     LAIFSINEIPF"
     gene            complement(6218..7804)
                     /gene="gspE"
                     /locus_tag="VEL68_23560"
     CDS             complement(6218..7804)
                     /gene="gspE"
                     /locus_tag="VEL68_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HXZ39026.1"
                     /translation="MVKTGLLTPENFPEAVQAFFGVPLAKDEDFPKEPTLYDNLPVQF
                     MKESKFIPAQMKDNTLTVIMADPNDFYTLDAIRLATRKQVQVLMGQESEIVAAIERSY
                     GSGGSSMEKIIEDIDGIPEYQAGDEENVDQLRDMASEGPVIRLVNLIITRAIERRSSD
                     IHMEPFEDHFRVRYRIDGVLHDVESPPKRLQAAIISRVKIMAKMNIAERRLPQDGRIM
                     LRVKGKEVDFRVSTVPTIHGESIVLRILDKSSIVLDILKLGFSRDTLEAFQRLVEHPH
                     GIILVTGPTGSGKTTTLYCALGKINSPDRKIITVEDPVEYQLQGINQIQVKPAINLTF
                     ANALRSIVRQDPDVILIGEIRDAETADIAIHSALTGHLVLSTLHTNDAPSAITRLINM
                     GMEDYLLSSTLIGILAQRLVRTACPYCQVPFPPDPAVVQEMKMDGMNPEEIRAFDVRG
                     CSQCANTGYWGRVGIFELLQVTEDIQKLILEKKDSNIIKERARRNGMRTLRQDGWLKV
                     REGITTVSEVLRVTQEEQLL"
     gene            complement(8305..9513)
                     /locus_tag="VEL68_23565"
     CDS             complement(8305..9513)
                     /locus_tag="VEL68_23565"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HXZ39027.1"
                     /translation="MKERDENFRSAILYSDELASFEYSPTHPFKPIRAKLTLDLCRRY
                     DLLERSWIRLVKPEPLDFETLAEFHDREYLKTLRDVDSATMAAEMWPDLIASGGRNYL
                     TVDPKILRFGLGTEDNPVFAGVYEYSALSAGATLQGAKMVAAGEVRVAFNLLGGFHHA
                     GRDHAEGFCYINDIAIAINRLLKEGLQVAFVDIDAHHANGVQDAFYRDPRVLVISFHE
                     NGKDLYPWSGFENEIGEGKGRGYTVNVPLPQNTDDEAFMRAFSAVVPPLLESYSPDLV
                     IAELGADTHISDPLTHLSMTNAGYCQAVKKLKEYSSRLLALGGGGYDVFKTARSWTLA
                     WGILNDLEPRDEYVGLVGGMMFGPEIEVGSLQDKTVYIKGSTREKVNQEIDRILAFLK
                     ENVFPIHKIK"
BASE COUNT         2098 a         2837 c         2534 g         2384 t
ORIGIN      
        1 cggcaatgcc gatgaggacc cagaggaatt tcttctggga taaggcggcc ttctttaaaa
       61 gggaccattt cacggtgtcc cccccaggcc ttcgatcctc attttgatct tgaaacgttc
      121 cctttccttg tccgctccga gccttctttc ccgttctttc gtgaccggag ccatgaattc
      181 caccttttcg aagatcgggg atttatccag cagcggaatg agctcggagg ccgaatcggc
      241 aaagccgcta atttcgatct ccttccccgc gaacttatat tgccagaccc aaaccgaaga
      301 gggaagaatt tggcttaact ctcgcaaaat ctcgacctcg ctgattgccc cccgtatgat
      361 tttatcgaac tcggcgatct cggcggcctc cttggttctt tgcttctgaa gtttttccac
      421 cgcatctgct tccggctttt tctttttcac ttccgctcgg agaacctcca gctcgttccg
      481 gtaacggctg taaatccccc ctccccaggt taggccaaga atgatcgcca tgcacaagag
      541 gatcaagaag gccttctttc cgtattgggg gattttcttc ctcatctctg gggggagaag
      601 attcaaacgg aagggggttt gcatcaggcc agtcagggga agtccgaggg aagggtagat
      661 ataatcggga agggtctcat cggggcccac ttcaatacgg cccatgggag gagaggcgac
      721 ccctttcatc ccatgggcct cacggaattg gggaagggta ttttcaattg catccagacc
      781 gtaaacaaaa aacatgccct tggacagaga aaggtcattc agttcggatc ttttgaaggc
      841 gcgaaggacc ccctcttcct tttcctcctt gggcagggga aggtggaagc tgtctttcca
      901 atccgctcct tcgagaatat tcatctcaaa atttgggtca ttcacgtcga ggagtacaga
      961 gacctcgttc ttcttctgct cttcgtggta gaagaagagg ttcagtgcgg atatggccga
     1021 aatctggatc gaaatgggtt ggatccccat ccttttcaag aggttcaaat agggagccag
     1081 gtcttctttt cttgcaaaga ccgcaatcag gtaaatccat tgggaatctt ctttcaagac
     1141 ctgaaaatcc gagtaaaatt catctttatc gaagggggtt agcttagggg cttcgtattc
     1201 caggaccttg gcaagattct ctttggcggc gaggggcaat cgcagaaaac gtaccaaggt
     1261 cgtttcccgg gggatggaaa cggagatcct ctcctgcccg cctctattct tgaagatgaa
     1321 ggtgcggatg aagctaatcc atttggcttc ttggttctcc ccttgtccat cccaccctaa
     1381 agggaagacc cggtagtcca ccaggcgaac cttgcggaat gacctcctca taagggtgag
     1441 gattaagtag ttggccttaa aatcgatccc caagctagat tgaaaatagg acatattttt
     1501 aagggttagc aaaaagtgta ccagattcat tcaagtattt gtaagaatta tttttttctc
     1561 attagagaaa atccagccat tggattattt tatatccatc ctgttctcgg gagtctattt
     1621 tgacgaccgc tcggatcccc ctgacagcac ccccctcctt ggtctttcct ctcgcttcta
     1681 cggtatagta tgggttcgtc gccgcgaaaa gaatcaactt ttggacctcg acgctgaatg
     1741 gcgaaagctc cggaacccgt tggagaaggt cgggaaagtt ttcaaaacct ttgtcctccc
     1801 gggcctttat gatttggcgg cagacctcca cgggcaggct gaggactacc ctcaagaccg
     1861 gaaggctggc gctgttgatg tcaatctgtt cgcctagggc atagagggaa aagatgtctt
     1921 ttaggccgat gggttcttcc tttggcacct ccccttctct tccctttgcc ttctggccgt
     1981 agaaaagctc gggggtgacc ccccggacga gcatcagctc ctctgcggag tcaaaatctc
     2041 cgttcttgca gtcgtacggt tctttcaggg attggtagta ttcgttctcc gccccgttga
     2101 cccgaacgaa atcgtccggg tctctccagt ccaggatgga atcgaccacg atgtcccgcg
     2161 cgtccatttc cagccccagg ttggccatga tcttccgcag catcccgtcg gatacccggt
     2221 tgatattcac cttgcctgct tcccccatca cccggacagc cccttcaacc ccttcaaagg
     2281 ccaccggata ttccctcccg tccgtcaccc attcattgta ttcgatcggg gtttcccctg
     2341 gtttgacgtt tttcttcaac tgctgaaacc tggggtcctg cttgaggatg agttccgcca
     2401 cggctcgttg caggcttccc tgcgccgctg cgtagagctt gagctgctca tggtaattgc
     2461 gggtgatgtt gacttccgtc cgcatggcaa aagaaaactc caggaccacg accgagagga
     2521 tcgtaatgat ccagagcacc atcaccaggg ccaccccccg cgatgcggca agctttggcg
     2581 acaacgattg aaacccacaa aaaattttca ttctgtcacg ccgcgtttat gggcccactc
     2641 ggccgcgggt cggaggtccg gtgctggcgc tgacgggtcc tctgctgacg ccctggctgc
     2701 ggatgtcctc gtagcggtta gccggaagag aagccagaat cttgaggggc gcctcttcgg
     2761 tccacccggc ccccttcagg gggaagaccg tcacccgcac ggccgtgggg agtgcattct
     2821 cctccttggc gctccactcc tccacccatc cctcagtttt gttcttattt gtatcctcct
     2881 cctgataata ctcgaagcga acatcgccca gcccctccag gagggcgatg gcagcttctt
     2941 cgcccggggg ctcgtcgagg aagttcttgt tcaggaccct cccttcgtaa aggatcaatt
     3001 gaccgcctcc ccgcccccct tcgcggaact catagaaggc atacaccaaa ccggaaggtt
     3061 tccttgcttt gagggaaacg gtagatacga atttcaaaga cctggctctt ccctcgaacg
     3121 ccaggaaatc tccctccgcc ttctgggtct ttaccttgta agcgatggcc gatttcgtct
     3181 gctgaaagat cagctgggaa acgatccggg cacgctggta ctcctccttg gtcgattccc
     3241 ctttctccca tgccgatagg cccaggcgga aggtcccgaa gatcatgagc agaagaaaac
     3301 cgaggatggt catggtgacc atcacctcga ccagggtgaa ggctgccgtc cagcctctgg
     3361 gccaggggcc tgtcctaggt cttttgccca ctctcttcct ctttcaacaa ccggtaagat
     3421 tcaatgctcg cgacccggtc tttcaaaccg gacctccaga gcacatcgat ccgcacccgg
     3481 taaagcgcca ccgaaggtcg gtaggtgatg tcttttcccg gcgggatgag gtccaccctc
     3541 ttcacctcca cctgccagcg atagttgctg tcgaattctc cctggtaaac cccctccgcc
     3601 aggctcttgt ccagggagat ttcttctatc tttatccgtg catacccggc ggccttggta
     3661 tattcctcgg aaaccctccc caggcgcaac ccccccccga agagttcgat gatgaccaca
     3721 agaccgatac tcaggatggc catggccacc acgacttcga tcagtgtaaa ccccggggct
     3781 tttttcctcc ggctcctcat gtcccctcga ttcccacgat tccggtcaga aaatggacct
     3841 tgatccagta gcctttttgg tcctgcatct ccagaaggac cgcccccccg ttcgatcctc
     3901 cggtcgggta gaattcgatg gtcggcaaat cggagggaaa ctcaggcgaa gggattctga
     3961 cctctttaat caggatcccc cccggcaggg gataccgcct ttcctgcgcc ggtgcttcct
     4021 cggtcccctt tttcccctct tcctgctttc tctccccttc cttctcctcc gcttccagag
     4081 accggatcct gatttcgccg gtacccgaat caaagagaat ctggtagacc ctccccttct
     4141 ggatggactc gctgcggaag tagcggagga tgcccgagac tctttgcgcc gtggccttga
     4201 gctcgactcc cttggagaac cgggacaggg agggagttac cagggcgatc gagaggctga
     4261 ccagtaccag cacgatcacc atctcgatca gggaaaagcc gctgtttctc atcccttgac
     4321 gtccttccag ctgaccacgt cttcgttttc cccttctccg cccgggaccc catccagccc
     4381 gtaggaaatg aggtcgtaat ctccttgctc tcccgggcat ttgtagatat agggtcttcc
     4441 ccaggggtcc aagggaatct ctttgggaag gtacggccct ttccaaaggt ccattgcgga
     4501 aggtttttcc cggagggcct tcaggccctc ttcgctggtc ggataccgtc ccacgtcaag
     4561 gcgcaacgca tcgagtgccg tgccgaagag ctcaatctgg gccttggccg cctttagctt
     4621 ggccattccc actttcccga aaagcctggg tcccaccaag gcggccagaa gcccgatgat
     4681 aatgatgacc accaggattt ccaccagggt gaatcctctt tgtttgtgac ctgcggccat
     4741 gctcctcctt ccacccgcgg aatccgggtt atagaaatct tttagcccta ataaagcgca
     4801 gggatgttta aattctgcga accgatttcc ctcatgaatg aagttctgtt tgcttccgat
     4861 tctgaataag agacaattcc aaaagagggg tatttccaaa tcggcatgaa taccgccaag
     4921 cggcttcctc tgttcccccc tttttcttcc tttccaaaaa gttaagaatc tctctgcctc
     4981 tattgcggcc aagaattaaa acggaatttc gttgatgctg aagatggcca ggagcatgga
     5041 gatcacgatg aatcccacca cggcccccat gaacaggatg atcagcggct ccagcaggga
     5101 gacaaaccgt ttcaccgtgg tttgaacatc tgcttcatac gtctccgcca ctttgctcaa
     5161 catttcttcc atgcgcccgg tctcctcccc cacgctgatc atgtgcacgg ccaggggggg
     5221 gaaaacgccc gtttcttcaa gcgttcgggc cacggctttt ccttccctca gtcggtcatg
     5281 gatgtttgcg atggcccggg acaccgccaa gttctgggaa atttctttca ccaggttgag
     5341 cgccgaaaga atggggactc cgctctgcag cagggtggcc agggtccggg aaaaccgggc
     5401 cacttcgatt ttcttgatga tgtttcctac cgccggccag cgcagtttgg aacggtccca
     5461 gcggaatctc ctctcctcct cctggttgta ggccctcaag aaaaaataga ttagggccag
     5521 catccccgca cctaaccacc agtagtcccg gaaaacttgg gagagggtga gcaggacctg
     5581 ggtcggcaag gggatggcct gccccatatc ggaaaagatt ttggcaaagc gggggatgac
     5641 aaaggtcacc aggattacga tggaggcccc gctcaccgcg gtcaggatga gggggtagat
     5701 catgaccgaa accaggaaat cccggagttc cttcgcgctc tgcaggtatt tgaccaggcg
     5761 ggaaaggatg acctccagaa aaccacctga ctcgccggct ttgaccatgt tgacgtagag
     5821 cttggagaag atacggggat ggtttcccag agcatcggcc agggaacttc cttcccggac
     5881 tcgattcaga atatcctgca cgaccccctt caatttgggg tttttggcca gggagcccag
     5941 gatttgaagg ctccggtcga tgggaagccc ggcggtaacc agggtggaaa gctcctggga
     6001 aagggccaga acgtgtttca atcccaccct ctgaggcagg aaggagaacc gggggctagc
     6061 ccctgattcc ccggcaagga tcaccctgac cggaatgtat cccagctcat gaagactatg
     6121 gacgaccgct ttttcgtcac ggccctccat ggagccttcc acaatctccc cgctggtttt
     6181 ggttgctttg tagacgaact cggccatctc cctgccctta caaaagctgt tcttcctggg
     6241 tgacccgaag gacttccgaa accgtggtga ttccctctct tactttcagc cagccatctt
     6301 gccgcagggt tcgcatgcca ttccttcggg ctctctcctt gatgatgttg gagtcttttt
     6361 tctccaggat gagcttctgg atgtcttcgg ttacctggag aagttcgaag atccccaccc
     6421 gcccccaata tccggtgttg gcgcactgag agcatcccct cacatcgaag gcccggattt
     6481 cctcgggatt catgccatcc atcttcatct cttgaacaac cgccgggtcg gggggaaaag
     6541 gaacctgaca gtagggacag gccgtgcgca ccagtcgctg ggccaggatg ccgatcaacg
     6601 tggaagagag cagataatcc tccatcccca tgttgatcag gcgggtgatg gcgctgggag
     6661 catcgttggt atgcagcgtc gaaagaacta ggtggccggt gagagctgaa tggatggcga
     6721 tatccgcggt ttctgcatcc cggatctccc cgatcaggat gacatcgggg tcctgtcgaa
     6781 cgatggaccg cagggcgtta gcgaaggtga ggttgatggc cggcttcacc tggatctggt
     6841 tgatcccttg caactgatac tccacggggt cttccacggt aatgatcttc ctgtccggcg
     6901 aattgatctt tcccagggcg cagtaaaggg tggtggtctt tccgcttccc gtaggaccgg
     6961 tgaccagaat gatcccgtgg gggtgttcga cgagcctctg gaaagcttcc agcgtatccc
     7021 ggctgaatcc caatttcagg atgtccagaa cgatgctgct cttatccaga atccgcagca
     7081 cgatagactc cccgtgaatc gttggcacag tggatacccg gaagtcgact tccttgcctt
     7141 tcacccggag catgatccgc ccgtcctggg ggaggcgacg ctcggcgatg ttcatcttgg
     7201 ccataatctt aacacgggaa ataatcgccg cctgcaggcg cttgggggga gattccacgt
     7261 catggagcac gccgtcgatt cggtagcgaa cccggaagtg gtcctcaaag ggctccatat
     7321 ggatgtcgga agacctcctc tcgatagccc gggtaatgat caggttgacc agacggataa
     7381 ccggaccttc cgatgccata tctctcagtt gatccacgtt ttcttcgtca ccggcctggt
     7441 attccgggat gccgtcgatg tcctcgatga tcttctccat ggaggaccct cccgacccgt
     7501 aggagcgttc gatcgccgcg acgatctccg actcttggcc catgagcact tggacctgct
     7561 tcctggtggc cagccgaata gcgtcaaggg tgtaaaagtc gttcgggtcg gccatgatta
     7621 cggtcagggt gttgtccttc atctgggccg ggatgaattt tgactctttc atgaattgaa
     7681 cgggaaggtt gtcataaagg gtcggttctt tagggaaatc ctcatctttg gcaaggggaa
     7741 ctccaaaaaa agcctggaca gcttctggaa aattttccgg agtcaggagc cccgttttta
     7801 ccaagttctc ctccactgaa aaatcggctg gaaggctccc tccgggaaag gtctcctcaa
     7861 aatctgctct gtgaatgaac ccctgatcca tcaaataatc aagaaattgc cgattcacca
     7921 tgatccccaa ccccttatgg attcttcatt tcctccccgg caggggaaaa aaggactccc
     7981 tttgggctgc gatcctgctc aaggatccat gtcccccatc gaaagaaaaa tttctccccc
     8041 cccaaacgac aaagcagaat tctcccctcc cgaaattata tattaaaatc gccaaccgat
     8101 gaaagataaa attagccgtc ttcggaccta tccattagat taatcggcta aggacctgtt
     8161 tttctgtaag aaagccttag aatctaatgt gcccttttga actttttgct atttctgggg
     8221 ctcagggaat atggagggaa agcatgtcct gggaggatca gaggtgaact cccttcatgt
     8281 atgaagtccg gagttctgaa cccgctactt gattttatgg atgggaaaga cattctcctt
     8341 gaggaaggct agaattcggt caatttcctg gttgaccttt tctctcgtcg accctttgat
     8401 gtagaccgtt ttgtcctgca aactgcccac ttcgatctcc ggtccgaaca tcatcccccc
     8461 caccagtcct acatactcat cccggggctc caggtcgttt agaatccccc aggccagggt
     8521 ccaactccgg gccgtcttga agacatcgta tcctccgccc cccagggcca gaaggcggga
     8581 agagtactcc ttcaattttt tcaccgcctg gcagtatccc gcattcgtca tactcaagtg
     8641 agtcaaagga tcggagatgt gggtatccgc ccccagttcg gcgatcacca agtcggggga
     8701 ataggattcc aggagggggg gaacgaccgc cgaaaaggcc ctcatgaatg cctcatcgtc
     8761 ggtattttgg gggaggggca cgtttaccgt atatcccctc ccctttcctt ctccgatctc
     8821 gttttcgaat cccgaccagg gatagaggtc tttcccgttc tcgtgaaaag agatcaccaa
     8881 gactcggggg tcacggtaaa aggcgtcttg aactccattg gcatggtggg catcaatgtc
     8941 cacaaaggct acctgcagtc cttctttcag gagacggttg atggcgatgg caatgtcgtt
     9001 aatgtagcag aacccttcgg catggtctcg gccggcgtga tgaaatcccc ccagaagatt
     9061 gaaggctacc cgaacctctc ccgcagccac cattttggcg ccctggagag tcgccccggc
     9121 cgacagggct gaatattcat agaccccggc gaaaacggga ttgtcctcgg ttcccaggcc
     9181 gaaacgcagg attttcgggt ctaccgtaag gtaattcctc cccccgctgg cgatcaagtc
     9241 gggccacatc tcagccgcca tggtggccga atccacatcc ctgagggttt tcaagtattc
     9301 ccggtcgtga aattctgcca gagtttcaaa atcgagaggt tcgggtttca ccaagcgaat
     9361 ccaggagcgt tcgagcaaat cataccgtcg acagaggtcc aaggtcagtt tggcgcgaat
     9421 gggcttaaag ggatgggttg ggctgtattc aaaactggcc agctcatcag agtaaagaat
     9481 ggcgctccta aaattttcat cccgttcttt catagcctcc ccatcataca caaatcctct
     9541 ctggggtcaa accattttct gtttccccac gcccctcgct tcggccacat cgcttttcga
     9601 ggcccggcgt caaggtcggg gctctcaggc aggacctgaa gggatcaata cttgcccatc
     9661 cgcagttttt aagaaaacag cccttcatcc ggtaaactga ggggaggggg gaagctcatg
     9721 ggattcaggg tgattgatac ttgccaaccc ccagtttttc gtgcttgttg gggttcaggg
     9781 tggaggaatt tggatcatca ggctcggcgg gtgcttatcc ttccatgaaa ctcctcccca
     9841 tgttccgcag gga
//
LOCUS       DASPRR010000643         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_642323, whole genome shotgun sequence.
ACCESSION   DASPRR010000643 DASPRR010000000
VERSION     DASPRR010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642323"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..983)
                     /locus_tag="VEL68_23570"
     CDS             complement(3..983)
                     /locus_tag="VEL68_23570"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HXZ39028.1"
                     /translation="MIRSRIIATGSYVPPRVLTNFDLEKMVDTSDEWITSRSGIRERR
                     IVEKDVATSDLGTQAALRALEMAGLSPEDLDFIITGTNSPDMFFPCTGCFIQAKIGAK
                     KAAAFDVSAGCTSFIHTLSLADKFIKEDPARKVMVLGAEIMSKVTDWSDRATCVLFGD
                     GAGAMILVGEEGDRGVLSTHLHSDGSLWELLYMPGGGSAHPSSHETVDQRLHCIKMAG
                     NPLFKVAVRALAEVSQEALKYNGLTSEDIDLMIPHQANTRIIEAAAKLINFPMDKVYL
                     NIEKYGNTSSATIPIALDELNRGGKLKAGNVILLCSFGTGVTWGGAVIRW"
     gene            complement(1069..2133)
                     /gene="plsX"
                     /locus_tag="VEL68_23575"
     CDS             complement(1069..2133)
                     /gene="plsX"
                     /locus_tag="VEL68_23575"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXZ39029.1"
                     /translation="MKIAVDAMGGDFAPQSIVEGAVLAAREHQIPVMLVGEEKTIRKE
                     VGRYAGSESLPLTFVHASEVVGMDESPLTPIRKKKDSSIKVAFDMLKKGEASAVVSAG
                     NSGAVLATAVFVLQKLNGVDRPAIGTIFPTLKGWTLLLDAGANVDCKPFHLVQFALMG
                     DAYAKHILKKERPVVGLLSNGEEESKGNELIRETNGILRKSSIGYIGPVEGRDIFNGR
                     ADVVICDGFVGNAALKICEGLAEAIGSMIRQELEESFRAKIGYLFARQAVSAIKKKLD
                     YSEYGGAPLLGVDGVVIIGHGRSSAKAIKNAVRLAYEFAQISLPMLLHRNLEENQDLQ
                     RLGGKNLAKVWEQIKEKIIG"
     gene            complement(2149..>2260)
                     /gene="rpmF"
                     /locus_tag="VEL68_23580"
     CDS             complement(2149..>2260)
                     /gene="rpmF"
                     /locus_tag="VEL68_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXZ39030.1"
                     /translation="PQFSACPQCGEPRAPHRVCPHCGSYKGRTVIEVEET"
BASE COUNT          438 a          694 c          610 g          518 t
ORIGIN      
        1 aattaccagc ggatgacggc ccctccccag gtcacccccg ttccaaaaga gcacaacagt
       61 attacattcc cggccttcag cttccccccc cggtttaatt catccagggc gatggggatc
      121 gtggccgaag aggtgttccc gtatttttcg atgttcaaat agaccttgtc catggggaaa
      181 ttgatgagtt tggccgctgc ttcgatgatg cgcgtgttcg cctggtgggg gatcatcagg
      241 tcgatgtctt cgctggtcaa gccattgtat ttcagagcct cctgcgatac ttccgccaga
      301 gcccgaacgg caactttgaa aagcgggttc cccgccatct tgatgcagtg caggcgctgg
      361 tccacagtct cgtgggagga tggatgcgcc gaaccccctc ccggcatgta gagaagctcc
      421 cacaaggaac cgtccgagtg caggtgggtg gaaaggaccc cccggtctcc ttcttctccc
      481 acgaggatca tcgcgccggc tccgtctccg aagaggacac aggtggcccg gtcggaccag
      541 tccgttactt tcgacatgat ctccgcgccc agaaccatca ccttacgggc cgggtcttcc
      601 ttgatgaatt tgtcagccaa ggaaagggta tggatgaaac tcgtgcatcc tgcggagacg
      661 tcgaaggcgg ccgccttttt ggccccgatc ttggcctgga tgaagcaccc ggtgcaggga
      721 aagaacatat ccgggctgtt ggtccctgta atgatgaagt ccaggtcctc gggtgacagg
      781 ccggccatct ccagggcccg gagggctgcc tgggttccca ggtccgaggt ggccacgtcc
      841 ttctccacaa tcctgcgctc gcggatcccg gaacgggagg taatccactc gtcggaggta
      901 tccaccattt tttctaaatc gaagttggtc aggaccctgg gaggaacgta agaacctgtg
      961 gcaattatcc ttgaccggat catataagca tattcccttt ccttactctt tccccttcca
     1021 cctatccaga ggataggagt cgaagcagat gcctgagggc gctccccttc agccgataat
     1081 tttctcttta atttgctccc agaccttggc caaattcttt ccgccaagcc gctggagatc
     1141 ttggttctct tccaggttgc ggtgcaggag catcggaagg ctgatttggg caaactcgta
     1201 ggccaagcga acggcatttt taatggcctt cgccgaggat cttccgtggc cgatgatcac
     1261 cacgccatcg actcccagaa ggggagcccc gccatattca gagtaatcca gtttcttctt
     1321 gatcgcgctc accgcctggc gggcgaagag gtagccgatc ttggcgcgga agctctcctc
     1381 cagctcctgg cgaatcatgg acccgatggc ctcggctagg ccctcgcaga ttttcagagc
     1441 cgcgttccct acgaacccgt cgcagatcac cacatcggcc cgcccgttaa agatatcccg
     1501 cccttccacc gggccgatgt aaccgatcga gctctttcgc agaataccgt tggtttcccg
     1561 gatcagctca tttcccttgg attcctcttc cccattgctc agcaaaccga ccacgggtcg
     1621 ttctttcttc aggatatgtt tggcgtacgc gtcgcccatg agggcgaatt ggaccaggtg
     1681 aaaaggctta cagtccacat tcgccccggc gtcgagcaaa agggtccagc ccttcagggt
     1741 gggaaagatg gtcccgatcg ccgggcggtc caccccattc aacttctgca gcacgaagac
     1801 cgccgtggct aagaccgccc cggaatttcc cgcgctcacc accgccgagg cttccccttt
     1861 tttgagcatg tcgaaggcca ctttgatgga agagtctttc tttttccgga tgggtgtcag
     1921 gggagactca tccatgccca ccacttccga ggcatgcaca aaagttaaag gaagggattc
     1981 gctccccgcg tacctcccga cttccttacg gatggtcttc tcctctccga ccagcattac
     2041 ggggatctgg tgctcccggg ctgccagcac ggctccttcc acgatgcttt ggggggcgaa
     2101 atctccaccc atggcgtcta cggcgatttt cattggcttg ggttcccctt aggtttcttc
     2161 gacttcaata accgtccgtc ctttgtaact gccacaatgg gggcataccc gatgcggcgc
     2221 cctgggctcc ccgcattgcg ggcaggccga gaattgaggg
//
LOCUS       DASPRR010000644         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1607645, whole genome shotgun sequence.
ACCESSION   DASPRR010000644 DASPRR010000000
VERSION     DASPRR010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607645"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..230)
                     /locus_tag="VEL68_23585"
     CDS             complement(6..230)
                     /locus_tag="VEL68_23585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39031.1"
                     /translation="MMLMSYEESYVKAFSFDKLAPGQMNLITNNRAGVQLAAAEKGRL
                     FVEEAVRRTVEYLKKMMEGEIGMPELKYFP"
     gene            complement(235..1062)
                     /locus_tag="VEL68_23590"
     CDS             complement(235..1062)
                     /locus_tag="VEL68_23590"
                     /inference="COORDINATES: protein motif:HMM:NF015949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HXZ39032.1"
                     /translation="MTSVIEETVDLLNKASDASYKDARIEDLVIGIFFTGVKLSTGHS
                     GVAFTPIGEIPEAVCCPTTAARMPQAGELDGKPAFEVLAYSLDQNVLKSAIGVATLNA
                     LSQPILERDGTTKYGMVKGTDGFDLLKIQPNETVSLIGAFGPYIRRLKIMGNRFYIIE
                     KNTQTLRPEEMKYFKPEVEMASALQESHVVIMTGTAIVNHTIDAILSCLSWRQRAAII
                     GPTASMVPDAFFKRGIQIMAGISIKDPDRMIQILKQGGSGYHLLQECSEKIALNFQR"
     gene            complement(1241..1492)
                     /locus_tag="VEL68_23595"
     CDS             complement(1241..1492)
                     /locus_tag="VEL68_23595"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXZ39033.1"
                     /translation="MDDSISITLRSYVLPPGSDDFPLGQPLVYRVSAGMVVGKLLEII
                     FGDRANQVGIVAVNGRVADGQTPLKDGDRVDAFEILGGG"
     gene            complement(1603..>2250)
                     /locus_tag="VEL68_23600"
     CDS             complement(1603..>2250)
                     /locus_tag="VEL68_23600"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HXZ39034.1"
                     /translation="RQIGRGAEELAIHVKGLEIPMHDPRSKQAMALHYSVHPTGADHC
                     SGVHDPVVEKGPGFDDWGTIDVNESIPSTELSPRKARMLYQVGMWTHLPNHLGFCQFV
                     PFKKKQLQQATEAITGWPMSYWKLMKAVERGLALAKIFNLREGFTRADDVLPKRMAVS
                     QTKGNLQGVIVDPGKLAEAQELYYQMLGWDSEGVPTRARLVELGIEWANSYIKGR"
BASE COUNT          481 a          636 c          594 g          539 t
ORIGIN      
        1 agaactcaag ggaaatattt tagttccggc attccaatct cgccctccat cattttcttt
       61 aagtattcaa ccgtccgtcg gaccgcttct tccacaaaga gccttccttt ttccgcggcc
      121 gccagctgca cgccggcgcg gttattggtg atgagattca tctgtccggg ggccagtttg
      181 tcgaaagaga aggccttcac ataggattcc tcgtagctca tcaacatcat cacttcatct
      241 ttgaaaatta agcgctattt tttcagagca ctcctggagc aggtgatagc ccgacccccc
      301 ttgcttaaga atttggatca tcctatcggg gtccttaatc gaaatcccag ccatgatttg
      361 gatccctctt ttgaaaaagg catcaggtac catgctggcg gtgggtccaa taatagcggc
      421 ccgctgcctc caactgaggc aggagaggat ggcatcaatg gtgtgattca caatcgcagt
      481 tccggtcata atgaccacat gggactcctg aagggcggag gccatttcca cttcaggctt
      541 aaaatacttc atctcttccg gtctcaaggt ttgggtattc ttttcgatta tatagaaccg
      601 gttccccatt atcttgagcc gacgaatgta aggaccaaag gcgccaatca aagagactgt
      661 ttcgttgggt tgaattttca agagatcaaa accatcggtt cccttcacca taccgtattt
      721 cgtagttcca tccctttcaa ggattggttg ggagagggcg ttgagggtgg ccactccaat
      781 ggcgcttttc aagacatttt ggtccaaaga ataggcgaga acttcgaaag caggtttccc
      841 atccaattct ccagcctggg gcattcttgc tgccgtagtg gggcagcaaa ccgcctctgg
      901 aatctcgccg atcggcgtaa aggctacgcc cgaatggcca gttgacagtt tcacgccggt
      961 gaaaaaaatg ccgatgacca ggtcttcaat cctggcatct ttataagacg catccgaggc
     1021 tttgttcagg agatcaacgg tctcctcgat cactgaggtc ataggttcct cccttaataa
     1081 gggcatattc aatttcctgg ctcgaacgca tgaagtttca tatttcctaa ccggttttcg
     1141 gttcaagagc gaagatatat tgagttcctg cagcgggggg ttgccctaaa tggaattgaa
     1201 tggctcccgg tcaacaacgc ctggccggct tttcaacctc tcaccccccg ccgagaattt
     1261 caaacgcgtc gacccggtcg ccgtccttca aaggcgtctg gccgtcggcc acccggccgt
     1321 tgaccgccac tatccccact tgattggccc gatcaccgaa tatgatctcc aggagctttc
     1381 ccaccaccat tccggcagat acccggtaga caagcggctg tccaagcggg aagtcgtcgg
     1441 acccgggggg caaaacataa gaacgaaggg tgatggagat ggaatcatcc ataaataaca
     1501 aggtacaggg tacacggcgc acggcacaag ccgcaagtcg taagtcgcaa gtcagtaaga
     1561 agtctttcct cgctctcgcg ccttacgctt agggatcttt cttcaccggc ctttgatgta
     1621 gctgttcgcc cattcgatgc cgagctccac taaccgggcc ctcgttggga ccccctcgga
     1681 atcccagccc agcatctggt agtagagttc ctgcgcttcg gccagtttcc cggggtcgac
     1741 aatcaccccc tggaggtttc ccttggtctg ggagaccgcc atccgcttgg gcaggacgtc
     1801 atcggcccgg gtgaaaccct cccggaggtt gaagatcttg gccagggcca ggccccgctc
     1861 cacggccttc atgagcttcc agtagctcat gggccagccg gtaatcgcct cggtggcctg
     1921 ctggagctgt ttcttcttga aggggacgaa ctggcagaag cccagatggt tggggaggtg
     1981 agtccacatt cccacctggt agagcatgcg ggctttgcgg gggctcaatt ccgtgctggg
     2041 gatggattcg ttgacgtcga tggtccccca gtcgtcgaac cctggtccct tttccacaac
     2101 cggatcgtga accccggagc agtggtccgc gccggtgggg tgcacgctgt agtgcagcgc
     2161 catggcctgc ttggagcggg gatcatgcat ggggatttcc agacccttga catggatggc
     2221 cagttcctcg gcgccgcgcc cgatctgccg
//
LOCUS       DASPRR010000645         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_307493, whole genome shotgun sequence.
ACCESSION   DASPRR010000645 DASPRR010000000
VERSION     DASPRR010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307493"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1505)
                     /locus_tag="VEL68_23605"
     CDS             complement(9..1505)
                     /locus_tag="VEL68_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ39035.1"
                     /translation="MDLVQNLLYGFSIALTPANLMYCFIGVFIGTLIGVLPGIGPVGA
                     MSLLLPTTLHVSPVGAIIMLAGIYYGAMYGGSTTSILLNIPGEAASVVTCIDGYQMAR
                     QGKAGPALGIAAFGSFIGGTASILGLMLVAPPLAEGALKFGPPEYFSLMILGMTLLIY
                     LASGSIPKALMMAVFGVILGTIGIDPTSGVQRFTFNILELNDGVGLVPLVMGLFGISE
                     VLLNVEQSLKREIFQAKIQGLLPDLDDWKKSAGPILRGSVLGFFLGILPGGGALISSF
                     VSYALEKKVSRTPERFGQGAIEGVAAPETANNAATGGAFIPLLTLGIPANVVMAMLFG
                     ALLIHGMQPGPMLLQSHPDLFWGVVTSMYVGNGMLLLLNLPLIGLWVQVLKVPYQILF
                     PLILLFCLIGAYSINYSSMDVLIMIVFGVFGYLMKKFEYEGAPLVLAYVLGPLLEMSL
                     RQSLTMSDGHFSIFFTRPISAVGIGVALLLVISPFLPWLGKKRPAMAE"
     gene            complement(1549..2022)
                     /locus_tag="VEL68_23610"
     CDS             complement(1549..2022)
                     /locus_tag="VEL68_23610"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ39036.1"
                     /translation="MGRADQFSSLFWLVLGLVVIILSYRLGLGTLTSPGPGFLPFWCA
                     LILSALSLGVFFHRLLIPSGGEPQKIRELWGGVRWLRGVFVVLALLGYTLVFSSLGYL
                     LSTLALLLFLFKTIEPQRWTVAIGGAVLASLISFVVFALWLDVQLPRGILERLLF"
BASE COUNT          627 a          613 c          686 g          417 t
ORIGIN      
        1 tattgttatt actctgccat tgccggcctc ttcttcccca gccaaggcag aaaaggggaa
       61 atgaccagca agagggcgac cccgattccg acggccgaga tcggccgagt gaagaaaata
      121 gaaaaatgtc cgtcggacat ggttagggac tgacgcagag acatttctag caagggacca
      181 aggacatacg cgagaaccag aggggcgccc tcgtactcga atttcttcat caggtagcca
      241 aagaccccga aaacgatcat gatgaggacg tccatggaac tgtagttgat gctgtaggca
      301 ccgatcaggc agaatagcag gatcaaggga aagaggatct ggtagggaac cttcagcacc
      361 tgcacccaga ggccgatcaa gggcagattg aggaggagaa gcatgccgtt gcccacgtac
      421 atgctggtca ccactcccca gaaaaggtcg gggtgactct ggaggagcat gggacctggc
      481 tgcatgccgt gaatgaggag ggctccgaag agcatggcca tgaccacatt cgccgggatt
      541 cccagagtca gaagggggat gaaggcaccg ccagtggccg cgttgttggc ggtctccggc
      601 gcggccacac cctcgatggc cccctgtccg aaccgctcgg gggtcctgga gacctttttc
      661 tccagggcgt aagagacaaa ggaagagatc agggctcctc ctccagggag gattcccaag
      721 aagaatccca ggaccgaccc ccgcagtata ggcccggccg atttcttcca gtcgtccagg
      781 tcgggcagga gtccctggat tttagcctgg aaaatctccc ttttcaggct ttgttccacg
      841 ttgagcagca cttcggaaat accgaagaga cccatcacca gggggacgag gccgaccccg
      901 tcgttcaatt ccaggatgtt gaaggtaaaa cgctgaaccc ccgatgtggg atcgattccg
      961 atcgttccca gaatgacgcc gaagacggcc atcatcaaag ccttggggat agaccccgag
     1021 gccaggtaga taagaagcgt catccccagg atcataaggc tgaaatattc cggcggcccg
     1081 aacttcaggg cgccctcggc gaggggcggg gcgacgagca tgaggcccag aatgcttgcc
     1141 gtccctccga tgaaggagcc gaaagccgca attcccagag ccggccctgc ttttccctgc
     1201 ctggccatct gatatccgtc tatgcaggtg accaccgatg cggcttctcc cgggatgttc
     1261 aggagaatgg aagtggtcga accgccgtac atggccccat agtagatgcc ggccaacatg
     1321 atgatggccc cgacggggga aacatggaga gtcgtgggaa gaaggaggga catggcgccc
     1381 accggaccga tccccgggag gactccaatg agagtgccaa tgaagactcc gataaagcag
     1441 tacatcaggt tcgccggagt cagggcaatt gaaaaaccgt aaagaagatt ctggactagg
     1501 tccattcttt ccctcccccc tccaagcgcg ggaagggggt gctgcgggtc aaaaaagtaa
     1561 tcgttccagg atgccccttg gaagctggac atcgagccaa agggcaaaaa ccacaaagct
     1621 gataagagag gctagaaccg ctcctcctat ggccaccgtc cacctttggg gttcgatggt
     1681 tttaaaaaga aagagcagaa gggcgagagt gctcaggaga tatcccaagc tgctgaatac
     1741 cagggtgtaa cccagaaggg ctaggactac gaagactccc ctaagccagc ggaccccacc
     1801 ccagagctcc cggatttttt ggggttctcc gcccgaaggg atgagcaggc gatggaagaa
     1861 gaccccgagg gaaagcgctg atagaatgag ggcacaccag aaaggaagaa atcccggccc
     1921 tgggctggtg agggttccga ggccaaggcg ataagacaga ataattacga ccaatcccag
     1981 cacaagccag aaaagggaac tgaactgatc agcccttccc attctctcct cccaggaaga
     2041 gaaaagaaat gccttttact taaacgttta ctccgacgca ggaatatcag aacggaaaaa
     2101 agatgtcaag aattttcggg gcttgccagt tgaggatggc gaaaaaggta actgaaccaa
     2161 aaattttctt gatgggtatc caaaagggtc agcctttttt gaagcttttc ctggacccga
     2221 tagccttcgt ggccgaggag accggaaagg aggtaatagg gcttggaata aaatacgtcc
     2281 tgatcggtga gctggtcaat cgcctggaag aacaggttgg cgatcaacaa atcggcatcg
     2341 ata
//
LOCUS       DASPRR010000646         8623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_477676, whole genome shotgun sequence.
ACCESSION   DASPRR010000646 DASPRR010000000
VERSION     DASPRR010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477676"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VEL68_23615"
     CDS             <1..564
                     /locus_tag="VEL68_23615"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXZ39037.1"
                     /translation="RLNTFGRHNVYNALGASALAAILGLDAEKIAAGLESFHPYPGRG
                     RIHPLGRGLRLLDDTYNSNPDSLQATLAAFGEMKGETRGLVVLGDMLELGSGTAGFHE
                     EAGRHVGALGFEHLFLCGEQREHLAEGAKSAGMEESRIHTFANPEEIAESLVSVVVEG
                     DWILVKGSRRLRMERVVEALIDRLRRS"
     gene            576..1652
                     /gene="mraY"
                     /locus_tag="VEL68_23620"
     CDS             576..1652
                     /gene="mraY"
                     /locus_tag="VEL68_23620"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXZ39038.1"
                     /translation="MLYTLLYALHSQYSLFNVFRYITFRAILAILTALVISFFLGPWV
                     IRKLKELQVKQYIREDGPKNHREKEGTPTMGGTLIIFSIAVAVLLWADLTNFFIWVAL
                     LVLAGFGAIGFADDYLKLIRRNSKGLSMKQKLLIQVLVALGASLLLYRNPTFKSTLAF
                     PFIKELMPDLGALYILLVILVIVGTSNAVNLTDGLDGLATGPVLISAGTFMVLSYLAG
                     NVKIASYLQIPYVSGSGELTIFCGALVGAAMGFLWYNTYPAQVFMGDTGSVSLGAALG
                     AVAVMTKQEFLLAIVGGLFVVETLSVIFQVASYKLRKKRIFRMAPIHHHFELLGWAEP
                     KVIVRFWIITVCLALLAFSTLKLR"
     gene            1774..3165
                     /gene="murD"
                     /locus_tag="VEL68_23625"
     CDS             1774..3165
                     /gene="murD"
                     /locus_tag="VEL68_23625"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXZ39039.1"
                     /translation="MDLKHKKVLVVGLARTGAATVRFLAQRGARVKGSEVKAAEEIKG
                     PLEALRGLHVEWEFGGHTLPFFLDAELIVISPGVPLSLPLLKKATEAGIPVISEIELA
                     FRFLRRPVIAITGTNGKTTTTTLIGEMLRASGQQAFVGGNIGNPLIQFVAGDQEEKWA
                     VVEVSSYQLEGIEKFRPAVAVLLNITADHLDRYPSFQAYGKAKGRVFENQGKEDYAVL
                     NADDALTFSFAHRVESQVLLFSRERPVPVGCFLDQGGILFQAAEGQRERYGLDRVKVR
                     GTHNLENLMAAISASRVCDCPPEALRKVMEEFEGLEHRLEWVRDIEGVRFFNDSKGTN
                     VGAVVKSLMSFQEPIWLIAGGRDKEGDYGPLNDLISGKVKGMALIGEAQKRMFEALGN
                     RTETVKAGSLEEAVEWAWARAKSGEVVLLSPACSSFDMFENYQERGRRFKEIVRGLKK
                     RPLPGAERKGRPL"
     gene            3167..4333
                     /gene="ftsW"
                     /locus_tag="VEL68_23630"
     CDS             3167..4333
                     /gene="ftsW"
                     /locus_tag="VEL68_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXZ39040.1"
                     /translation="MDFIRRMDPLLLLSTLALVGFGIVMVYSASFPVGTERMGDPYHF
                     LKKQAIAAALGIGLLIFGARMNYRYWQPLALPLLFGSIVLLFLVFVPGLRQQIGGAYR
                     WLKIYGFSFQPSELAKLALVIYLARSMTKKEGRMDRFTVGFLPFVLVLAILFPLVLKQ
                     PDFGTGVLFVALVFLMLFVAGTPLRFLGGALLATAPILVYVTMKAEYRWERILVFLDP
                     WKDSGKAGFQIIQSFLAFGAGKVFGVGLGEGKQKLFYLPEIHTDFIFSVIGEELGLIG
                     VTIIIALFLLVVIRGFQSCFRAPDRFGMYLSLGITVLITFQALLNMGVVMGLLPTKGS
                     TLPFISYGGTSLMMNLMAVGILLNVHSQGVRSKNEGPDHRRGNGRTSLPGPGRG"
     gene            4269..5354
                     /gene="murG"
                     /locus_tag="VEL68_23635"
     CDS             4269..5354
                     /gene="murG"
                     /locus_tag="VEL68_23635"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXZ39041.1"
                     /translation="MKVLITGGGTGGHLFPALAVAEAFRERDPETQIVFVGSRSGLEA
                     SLVARQGYELKAIEVAAWKGKGVGGKLGSLLAIPKSLWQSWRLLRSVQPGLVLGVGGY
                     ASGPVVLTAWAMGFRTAIHEQNSFPGLSNRILGRFVNRVFTSFEDSGDHFPRGKTALT
                     GNPVRRELQRERGASQSKGEKEFTLFIFGGSQGAHRLNQAMEESLPHLGDLRGKMRII
                     HQTGNLDYDHVRAFYKKEGVAAEVHRFIHDMGQAYRAADLILCRAGATTLFELMAMGK
                     AAILVPYPYAANDHQTLNANALVNAGAALMVANADLNGEVLSRLVRELKEDPDRRKRM
                     GERATALAQPGAAEKIVDLCYRMVTNE"
     gene            5368..6753
                     /gene="murC"
                     /locus_tag="VEL68_23640"
     CDS             5368..6753
                     /gene="murC"
                     /locus_tag="VEL68_23640"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXZ39042.1"
                     /translation="MRHRVRKVHFVGIGGIGMSGIAEVLLNLGYRVSGSDLAESETTH
                     RLQRLGAEVFLDHRAENLKEADVVVTSSAVRKENPEVLAAQQRLIPVIPRAEMLAELM
                     RMKYGIAIAGTHGKTTTTSMIATVLAHGGLDPTAVIGGKLNAFGSNAKLGQGDLLVAE
                     ADESDGSFLRLTPTIAVVTNIDPEHLDHYRNLEEIKEAFLEFINKVPFYGLAVLCLDH
                     ENVQALIPRVAKRFSTYGLTTQANYRATDISFNGLTTTFRAFENEQELGLLSIRMPGL
                     HSVYNALATLATALELDLGFEVVEEALGSFSGVQRRFQVKGEWGGVMVVDDYGHHPAE
                     IKATLSAAKRGWGRRTVVVFQPHRYTRTRDLFKDFLTAFNQADVLFLTGIYAASEDPL
                     PGVNVEDLSEGIKGHGHKDVTLVLEKDRIVEHLLPRLRPGDMVFTLGAGDVWKVGEAL
                     IKRLKERDSSE"
     gene            6816..7739
                     /gene="murB"
                     /locus_tag="VEL68_23645"
     CDS             6816..7739
                     /gene="murB"
                     /locus_tag="VEL68_23645"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXZ39043.1"
                     /translation="MTAASGIKLAEEIKGEVLYSVPMSQYTSFGVGGPVDFLIFPADL
                     EELKKTLQWCRRQKVSYFLLGNGSNLLVRDGGIRGAAISLARGFQGMAELERGETDGL
                     LQAGAAESLARLLEFCREKGLTGMEWAVGIPGTVGGALCMNAGAFRSEMKDNLASLQV
                     MDGEGNVIEKGREELHFSYRSLELKKGWIILGGKFRLKIGNGQAIRSKMDEFLKKRMA
                     KQPLHLPSAGSVFKNPSRVSAGQLIEEVGLKGTRLRDAQVSEKHANFIVNLGKAKARD
                     ILTLAEWAREKVYQEKGVKLEMEVQVIGEDE"
     gene            7839..>8623
                     /locus_tag="VEL68_23650"
     CDS             7839..>8623
                     /locus_tag="VEL68_23650"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXZ39044.1"
                     /translation="MKKKRIGVLMGGMSPEREVSLMSGKAILQALLEQGYPAVPIEAD
                     ETLSERLREAGVEVAFIGLHGSLGEDGSVQGLLEMMRIPYTGSGVLASALAINKAVSR
                     QVFLQNGLPVPRSVFLAQPPEGGVSRLKIPFSLPVVIKPCQEGSSVGVSIVSDRQDIP
                     PAAERAFGYGGGILVEEFIKGREIAVGILDELALGAIEIVPKAEFYTYEAKYTDGLAE
                     HLFPAPFPPRKYKKALDLGYQAHRLLGCEGATRVDLLYREPGE"
BASE COUNT         1808 a         2264 c         2741 g         1810 t
ORIGIN      
        1 cggctcaata ccttcgggag gcacaatgta tacaacgctc tgggggcttc ggctctggcc
       61 gccattctgg gattagacgc cgagaagatc gcggccgggc tggaaagctt ccacccgtat
      121 cccggacgag ggagaatcca cccgttgggc cggggcctac gtcttctgga tgacacctac
      181 aactccaacc cggattctct gcaggctacc ctggcggcct tcggagaaat gaagggggag
      241 acccggggat tggtggtttt gggggacatg ttagagcttg gatcggggac tgcgggtttc
      301 catgaggagg cgggaaggca tgtgggggcc ctggggttcg aacatctctt cctttgcggg
      361 gaacagcggg aacatctggc tgagggggcg aaatccgcgg gaatggagga gagccggatt
      421 cacacctttg ccaacccgga agagatcgcc gagagcctgg tgagtgtggt ggtcgagggg
      481 gactggattc tggtcaaagg gtcccgccga ttgaggatgg aaagagtggt ggaggctttg
      541 atcgatcgcc tgcggagatc atgaaggact gagcgatgct atatactttg ctttacgccc
      601 tgcatagcca atattctctt ttcaatgtct tccggtacat cactttccgg gcgatcctgg
      661 ccattctcac cgccctggtg atctcttttt tcctcggtcc ttgggtgatc cggaagctga
      721 aggaactgca ggtgaagcag tacatccgcg aagatgggcc gaaaaaccat cgggagaagg
      781 aagggacccc caccatgggt ggtacgttga tcatcttttc catcgcggtt gccgtcctcc
      841 tctgggccga tctcaccaat ttcttcatct gggttgccct gctggtgctg gcggggttcg
      901 gggcgatcgg ctttgccgac gattatctga agctcatccg caggaattcc aaaggattga
      961 gcatgaagca gaagttgctt atccaggtcc tggtggccct gggggcatcg ctgctgctct
     1021 acaggaatcc gaccttcaag agcaccctgg cctttccctt catcaaggaa ctcatgcccg
     1081 atctaggggc gctatacatt ctgctggtga tcttggtgat cgtgggaact tccaatgcgg
     1141 tgaatctgac cgacggcttg gacggtctgg ccacgggccc ggttctcatc tcggcgggca
     1201 ctttcatggt gctatcctac ctggcgggta acgtgaagat cgccagctac ctgcaaattc
     1261 cctatgtctc gggaagcgga gagctgacca ttttctgcgg ggccctggtc ggggcagcca
     1321 tgggcttcct ctggtacaac acctacccgg cgcaggtttt catgggcgat acaggctcgg
     1381 tctcgctggg ggcggctttg ggtgcggtgg cggttatgac caagcaggag ttcctcctgg
     1441 ccatcgtggg gggtcttttt gtggtggaaa ccctatcagt catcttccag gtcgcttcct
     1501 acaaactgcg gaagaagaga atttttcgga tggccccgat ccatcatcat tttgaacttt
     1561 tgggctgggc cgagcccaaa gtgatcgtcc ggttttggat tatcaccgtc tgtctggcgc
     1621 tgctggcgtt cagtacgttg aagctgcgct agaacgcgca tagagcattg ggcatagcgg
     1681 aaagaaaggt gtaaggatga gcagattgct ggtggacgat acctggattt ccaatccgca
     1741 atccgcaatc cgcaatccgc aatcatgaac gggatggacc tgaagcacaa aaaggtgcta
     1801 gtggtcggtc tggcccgcac gggcgcggcc acggtgcgct ttctggccca aaggggagcc
     1861 cgagtaaaag ggtcggaggt gaaagccgca gaagagatca aggggcccct ggaggcgctc
     1921 cgagggcttc atgtggaatg ggagttcgga gggcacaccc tgcccttttt cctggatgcc
     1981 gagctcatcg tgatcagccc gggagttccc ctgagccttc ccctgctgaa gaaagcaacc
     2041 gaagcgggaa ttccggtgat cagcgagatc gaactggctt tccgattcct gcgccggccg
     2101 gtgatcgcca tcaccgggac caacggcaag accaccacca ccaccctgat cggagagatg
     2161 ctccgagcca gcggacaaca agcctttgta gggggaaaca tcggaaaccc gctcatccaa
     2221 ttcgtggccg gagaccagga ggagaagtgg gctgtcgttg aagtgagcag ctaccagctg
     2281 gaggggatcg agaagttccg gccggcggtc gcggttctcc tcaacatcac cgcggaccac
     2341 ctggaccgtt atcccagctt tcaggcctac gggaaggcca aaggccgggt cttcgagaat
     2401 caggggaaag aagattacgc ggtcctcaac gccgacgacg ctcttacctt ttccttcgct
     2461 catcgggtag agtcccaggt ccttctcttc agccgggaga ggccggtgcc agtaggatgc
     2521 ttcctggatc aaggcgggat tctattccag gccgcggagg gccagaggga acggtatggc
     2581 ctcgaccggg tgaaggtccg agggacgcac aatctggaga acctgatggc ggccatctcc
     2641 gcctcccgag tctgtgattg cccaccggag gcgttgcgga aggtgatgga ggagtttgag
     2701 ggcctcgagc accgcttgga atgggtgcgg gacatagagg gggtgcggtt cttcaacgat
     2761 tccaagggga ccaatgtagg tgccgtagtc aaatcactca tgagtttcca ggaaccgatc
     2821 tggctgattg ccgggggtcg ggacaaagaa ggggattatg gcccgttgaa tgacctcatc
     2881 agcgggaagg tgaaaggaat ggctctcatc ggtgaggccc aaaaacgaat gtttgaggct
     2941 ttgggaaatc ggaccgagac ggtgaaggca ggttcgctgg aagaagcagt ggagtgggcc
     3001 tgggccaggg ccaagtccgg agaggtggtc cttctatcgc cggcttgttc gagtttcgac
     3061 atgttcgaaa actatcagga gcgcggtcgg agattcaagg agatcgtccg cggattaaaa
     3121 aaaaggcccc tgcccggggc agaaagaaaa ggaaggcctc tctgacatgg acttcatccg
     3181 gcggatggac cccttgcttc tcttatcgac cctggcttta gtggggttcg ggatcgtcat
     3241 ggtctatagc gccagcttcc cggtaggcac ggagcgcatg ggtgacccct accacttcct
     3301 caagaaacaa gccatcgcag cggccctggg gatcggtctc ctgattttcg gagcccgaat
     3361 gaattaccgc tattggcagc ccttggccct tcccctgctt ttcgggagca tcgttcttct
     3421 atttctggtt tttgtcccgg ggctgcgcca acagatcggg ggggcctacc ggtggctgaa
     3481 aatttatggt ttctctttcc agccttcgga gctggccaaa ctagccctgg tcatctacct
     3541 ggctcggtcc atgaccaaga aggaagggag gatggaccgg ttcaccgtag ggtttctccc
     3601 ctttgtctta gtcttggcaa tcctcttccc cctggttctg aagcagccgg atttcggcac
     3661 cggggtcctc tttgttgccc tggtgtttct tatgcttttt gtggccggaa cgccccttcg
     3721 ttttttggga ggggcgctcc tagcgacggc gccgatcttg gtgtatgtaa cgatgaaggc
     3781 tgagtaccgc tgggaacgga tactcgtctt cctggatcct tggaaagact cggggaaagc
     3841 ggggttccag atcattcaat ccttcctggc tttcggtgcg gggaaggtct tcggggtggg
     3901 gttgggggag ggaaagcaaa aactcttcta tttgccggag atccatacag acttcatctt
     3961 ctcggttatc ggggaggaac tgggcctgat cggggtaacg atcatcatcg cccttttcct
     4021 cctcgtggtc atccgaggct ttcagtcctg cttccgggcc cccgaccgat tcggtatgta
     4081 tctctctttg ggaattaccg tgctgatcac cttccaggcc ctcctcaaca tgggagtggt
     4141 catgggactc ttgcccacca aagggtccac tctgcctttt atcagctacg gagggacttc
     4201 gttgatgatg aacctgatgg ccgtggggat tcttctcaat gtacattctc agggcgtgag
     4261 atcaaagaat gaaggtcctg atcaccggcg ggggaacggg cggacatctc ttcccggccc
     4321 tggccgtggc tgaggcgttc cgggagcgag acccggaaac tcagatcgtt ttcgtgggga
     4381 gccggagcgg actggaggcc agccttgtgg cccgccaagg gtacgagctg aaggccatcg
     4441 aggtggccgc ctggaaaggg aaaggagtgg gagggaagct gggcagcctg ctggccattc
     4501 ccaagagcct ttggcagtcg tggagactgc tccggtcggt tcaacccgga ttggtgctgg
     4561 gagtgggagg ttacgcctcg gggccggtgg tccttaccgc ctgggccatg ggctttcgga
     4621 cggcgatcca cgagcagaac tcgttccctg ggctgagcaa tcgaattctg ggaaggttcg
     4681 taaaccgggt cttcacctcc tttgaagatt ccggggatca tttccccagg ggcaaaaccg
     4741 ccctcactgg caacccggtg agaagagaac tgcaacggga aaggggggct tctcagagca
     4801 agggggagaa agagttcacc cttttcattt tcgggggaag ccagggggcg caccgcttga
     4861 accaggccat ggaggagagc ctgccgcacc tcggggacct gaggggaaag atgcggatca
     4921 tccatcaaac cggcaacctt gactatgatc atgtccgggc cttttacaag aaagaagggg
     4981 tggcagccga ggtccatcgg tttatccacg acatgggaca ggcttaccgc gccgcggacc
     5041 tgatcctctg ccgggccggg gccaccaccc tcttcgaact catggctatg ggcaaagcgg
     5101 ctatcttggt cccttaccct tacgcggcca acgaccatca gaccctgaac gccaatgccc
     5161 tggtcaatgc cggcgccgcc ctcatggtgg ccaatgccga tttgaacgga gaggttctga
     5221 gccgcctggt gcgggagctg aaggaagacc cggataggcg gaagcggatg ggcgaaaggg
     5281 ccacggcctt ggcccagccc ggggccgcag agaaaatcgt ggacctctgc taccggatgg
     5341 tgacgaatga ataaccagac ctatggaatg aggcaccggg tccggaaggt ccacttcgtg
     5401 ggcatcggcg ggatcggcat gagcgggatc gccgaggttc tgttgaacct ggggtaccgg
     5461 gtgagcggtt cggacctggc cgagtccgaa acaacccacc ggctgcagag gttgggggcg
     5521 gaagtgttcc tcgaccatcg ggcggaaaat ttaaaagagg ccgacgtggt cgtcacctcc
     5581 tcggcggtcc gcaaggagaa tcctgaagtc ctcgccgccc agcagcggtt gatcccggtc
     5641 atcccccggg ccgagatgct ggccgaactg atgcggatga aatatggaat tgccattgcc
     5701 ggaacccatg ggaagaccac gaccacgtcc atgatcgcca cggtcctggc tcacggagga
     5761 ttggacccta cggcggtcat cgggggaaag ctgaatgcct tcggctccaa tgccaaactg
     5821 gggcagggag accttctggt ggcggaggcc gacgagagcg acgggtcctt tctcagattg
     5881 actccgacca ttgccgtggt gaccaacata gaccccgagc acctggacca ttaccgaaac
     5941 ctggaagaga tcaaagaagc cttcctcgag ttcattaaca aagtcccgtt ctatggcctg
     6001 gccgttctat gtttggacca cgagaacgtt caagccttga ttcccagggt ggccaaacga
     6061 ttctcaacct acggccttac cacccaggcc aattaccggg caacggatat ttctttcaac
     6121 ggcctcacca ccaccttccg ggcctttgag aatgagcagg aattggggtt gctgagcatc
     6181 cgcatgccgg gtctgcacag cgtctataat gccctggcca ccctggccac ggccttggaa
     6241 ctggaccttg gctttgaagt ggtcgaggaa gcgctggggt ctttcagcgg agtccagcga
     6301 agatttcagg tgaaagggga atggggcgga gtgatggtgg ttgatgacta tggccaccac
     6361 ccggcagaga tcaaagcgac cctgagcgcg gccaagagag ggtggggacg gagaacggtg
     6421 gtggtcttcc agccccaccg gtacacccgg acccgggacc ttttcaagga cttccttacc
     6481 gccttcaatc aagccgatgt tctcttcctg acgggaatct atgccgccag cgaggacccc
     6541 cttccaggag tgaacgtgga ggacctctcc gaaggcatca aaggccacgg ccacaaggat
     6601 gtgaccctgg tcctggaaaa agataggatt gtcgaacacc tcctcccccg cctacggccc
     6661 ggggacatgg tcttcaccct gggagcgggg gatgtttgga aggtagggga agctttaatc
     6721 aagaggttaa aggagagaga cagttcggag taatgtaacg ggtaggggcg ggttttaaac
     6781 ccgcccctac tgaatcccga gctcataaaa gtctcatgac tgcggcgagt ggaataaagc
     6841 tggcggaaga aattaaaggg gaggtcctat attccgtccc catgtcccag tacacttcct
     6901 ttggagtggg gggaccagta gatttcctta ttttcccggc agacctggaa gaattgaaga
     6961 agacccttca atggtgccgc cggcagaagg tatcttattt ccttctcggg aatgggagca
     7021 acctgctggt ccgggatgga ggaattcggg gagcagccat ctccttggcc cgcggcttcc
     7081 agggaatggc cgagttggaa cggggggaga cagatgggct cctacaggct ggagccgcag
     7141 aatcactggc gaggctctta gagttttgcc gggagaaagg cctgaccggg atggaatggg
     7201 ccgtaggaat tcccgggacc gtggggggcg ccctttgcat gaatgccggg gcgttccgga
     7261 gcgaaatgaa ggacaacctg gcgtccctcc aggtcatgga cggggaagga aacgtaattg
     7321 aaaagggaag agaagagctc catttttctt accgatcttt ggaacttaag aagggatgga
     7381 tcatcctggg gggtaaattc aggctcaaaa tcgggaatgg ccaagccatc cggtccaaga
     7441 tggacgaatt tttaaagaag agaatggcca aacagcctct ccacctcccg agcgccggat
     7501 cggtgttcaa aaacccatcc cgggtctcgg ccggccagtt gatcgaagaa gtggggctaa
     7561 aggggacccg cctgcgggat gcccaggttt cggagaagca tgccaacttt atcgtgaacc
     7621 tgggaaaagc caaggcgcgg gatatcctga ccctggccga atgggccagg gagaaggtgt
     7681 accaggaaaa gggtgtgaaa ctggagatgg aggtccaggt cataggggaa gacgagtaga
     7741 ataaagtgcg ttgagtgcct gaagtgagct aacgtgccta tagtttaaaa aattcatttt
     7801 aggcttttcg aattgccggt atgtttaaca aagaagagat gaagaaaaaa aggatcgggg
     7861 ttctgatggg cgggatgtcc cccgaacgcg aggtttcgct catgtccggg aaggccattc
     7921 tccaggcgct gttggaacag gggtacccgg ctgtgcccat cgaggccgac gaaactctct
     7981 ccgagcgtct ccgagaagcg ggggtcgaag tggccttcat cgggctccat ggttctctgg
     8041 gggaggacgg gtcggttcaa ggccttctgg agatgatgcg gattccctac accggctcgg
     8101 gggtcctggc cagcgctctg gccattaaca aggcggtctc ccgccaggtt tttttgcaga
     8161 acggtctgcc cgttccccgg tccgttttcc tggcccaacc cccggagggg ggggtctccc
     8221 ggttgaagat tcccttttcc ctcccggtgg ttattaagcc atgccaggaa ggatcgagcg
     8281 tgggggtcag catcgtctct gaccgccagg atattccccc ggctgcggag agggccttcg
     8341 ggtacggagg aggaatcctg gtggaagagt ttattaaagg acgggagatc gcggtgggca
     8401 tcctggatga gcttgctttg ggggccattg aaattgttcc caaggccgaa ttttacactt
     8461 atgaagccaa gtacaccgac ggactggcgg aacatttgtt ccctgcgccc tttcctccga
     8521 ggaaatacaa gaaggctttg gacttggggt atcaagccca ccggcttctg ggctgtgaag
     8581 gggcaacacg ggtggacctc ctttaccggg agcccggaga att
//
LOCUS       DASPRR010000647         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_477773, whole genome shotgun sequence.
ACCESSION   DASPRR010000647 DASPRR010000000
VERSION     DASPRR010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477773"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VEL68_23655"
     CDS             complement(<1..1294)
                     /locus_tag="VEL68_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HXZ39045.1"
                     /translation="MSKEKSLHMTPDEFRRYGRAAVDWIADYYERVESLPVLSQVRPG
                     QTRALLPSQPPIQGEPFEKILEDMNRVVLPGITHWQSPNFFAYFPANASGPSILGDLL
                     SSGLGVQGMLWSTSPACTEVETHVLDWTVDMMGLPAKFKSTGTGGGVIQDSASSASLC
                     ALLAARERATKFVSNEKGCDGRLVAYASTQTHSSVEKAVKIAGLGRQNLRLIDVDEKF
                     ALRPELLADQVRKDRKDGLIPCYVCATIGTTSSNAIDPLPEIGKVCRQEGIWLHVDGA
                     MLGTAAVCPEYRYLQDGIEYADSYCFNPHKWMFTNFDCDCFFVSDRAALIHTLSVLPE
                     YLRNQATESGAVIDYRDWHIPLGRRFRSLKLWFVIRHYGVEGLRTLIRRHVEWAQKFA
                     RWVQEDKRFELAVPPPMNLVCFRHVGGDGVNQKIMDRLN"
     gene            complement(1449..2498)
                     /locus_tag="VEL68_23660"
     CDS             complement(1449..2498)
                     /locus_tag="VEL68_23660"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HXZ39046.1"
                     /translation="MKLNTLPRVRLANLPTPLQELPNLTKALKGPRIFVKRDDLTGLA
                     FGGNKTRKLEYLMGEAVQQKADCIVTHAGFHSNWLTQTAAAARKLGMKIFMVKTGPHD
                     GYEPEEYDGNHLLHFLAGAVMKVVRPEKAADAVEETAFELRAAGHRPFVLRDMGSTPP
                     GVAGYVNFFRELDSQISDLGLDSIYLVHTTGAGGTQAGLIIGTKAYNTGIRVIGSTAG
                     SRTTAEQTPKVLNLIRESLQYLEIDVKVGIEDIVIYDNYAEGYGYATEKKAEAVRILA
                     ELEGLFLDPVYTASSMACLIDLARKGFFKKDDAVIFLHTGGSAALFAYKEPLRAFAEG
                     QKAPWTVPPWSPDRH"
     gene            2595..2834
                     /locus_tag="VEL68_23665"
     CDS             2595..2834
                     /locus_tag="VEL68_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39047.1"
                     /translation="MVNPQLCQWIISVRLFRDASVVARGRGASGQKASAGLALFGEAS
                     AGFRRMRRADRAIGRSALRLENAFRGVWMASPPDP"
BASE COUNT          606 a          899 c          886 g          701 t
ORIGIN      
        1 ggttcagtcg atccatgatc ttctggttga caccgtcccc tcctacatgg cggaagcaaa
       61 ccagattcat gggagggggt acagccagtt cgaaccgctt gtcctcttga acccaccggg
      121 cgaatttttg ggcccactcc acatgccgcc ggatgagggt acgcagccct tccaccccat
      181 agtggcgaat cacgaaccag agcttcaggg aacggaagcg ccttcccagg gggatatgcc
      241 agtcccgata gtcgatgacc gcccccgact cggtggcctg attgcgcaga tattccggga
      301 gaacgctaag ggtatggatc agcgccgccc ggtcggatac aaaaaagcag tcgcagtcga
      361 aattggtgaa catccacttg tgggggttga aacagtagct gtcggcgtat tcgataccgt
      421 cctgcagata gcgatactcg ggacatactg ccgccgtgcc cagcatggct ccgtccacgt
      481 gcagccagat tccttcctgc cggcagacct tcccgatctc cggaaggggg tcgatggcgt
      541 tggaagaggt cgtgccaatg gtggcacaga catagcaggg aatcaagccg tccttccgat
      601 cttttcggac ctggtcagcc agcagctccg gccggagggc gaatttttca tctacatcga
      661 tgagtcgcag gttctgccgg cccaggccgg cgattttcac agctttttcc acggaggagt
      721 gagtctgggt ggaggcgtag gccaccagcc gtccatcgca tcccttttca ttgctcacaa
      781 atttcgtcgc ccgctcccgc gccgccagca gtgcgcagag ggaggcgctg gaggcgctgt
      841 cctggattac tccccccccg gtgccggtgg acttgaattt cgccggcagg cccatcatat
      901 ccaccgtcca gtccaggaca tgggtctcca cctcggtgca ggccggactc gtggaccaga
      961 gcattccctg aaccccgagg ccggaagaaa gtaggtctcc gagaatggag gggccagaag
     1021 cattggcggg aaagtaggcg aagaaattgg gcgactgcca gtgggtaatg cccgggagga
     1081 ccacccggtt catatcttcg aggatttttt caaagggctc gccctggatc ggtggctgcg
     1141 aggggagcaa ggcgcgagtc tgtcccggtc tgacctggga caaaaccggg agcgattcaa
     1201 cccgctcata ataatcggcg atccagtcga ctgccgcccg gccgtagcgt cggaattcat
     1261 ctggggtcat gtgcaaggat ttttcctttg acatcgtttt ttctcccaca tttctgtaat
     1321 atctaaggaa tccccccggc tctgccgagg gacccataaa accagacgat tccggattgc
     1381 ccgagtcgtg gcccgggggg caagggcctg cacttttgcg aaggccgtct catgagctca
     1441 tgaccttttc aatgccgatc gggcgaccag ggcggaacgg tccagggggc cttttggccc
     1501 tcagcaaagg cccgcaaggg ctctttgtaa gcaaagaggg cagccgagcc gccggtgtgc
     1561 aggaatatga ccgcatcatc cttcttgaag aatcccttcc gtgccaggtc gattagacag
     1621 gccatggagg aggcggtgta gacggggtcc aggaagaggc cctccaattc agcaagaatc
     1681 cgtaccgcct ccgccttctt ttccgtagcg tatccgtaac cctcggcata gttgtcatat
     1741 attacgatgt cctctattcc gacttttacg tctatttcca ggtattggag cgattctctt
     1801 atcaggttga ggaccttggg ggtctgttcg gctgtcgtcc tcgacccggc ggtcgaaccg
     1861 atcacccgga tgccggtgtt gtaagctttg gtcccgatga tcaaacccgc ctgggttccc
     1921 cctgccccgg tggtgtgaac tagatatatc gagtctaaac caagatctga aatttggcta
     1981 tctaattcac ggaaaaaatt aacatatcct gccactccag gcggcgtgga ccccatatcc
     2041 cgcaggacga agggccgatg tcctgccgcc cgcaattcaa atgcggtttc ctcaaccgca
     2101 tccgcggctt tctcgggacg aacgactttc atgaccgccc cagccaggaa gtggagaagg
     2161 tggtttccat cgtattcctc tggctcgtaa ccgtcgtgag ggccggtttt aaccatgaag
     2221 attttcatcc ccagcttccg agctgccgca gcggtttggg tcagccaatt ggaatgaaac
     2281 ccggcatgag tcacgatgca atccgccttc tgctgcaccg cctcgcccat gaggtattcc
     2341 agcttccggg tcttgtttcc cccgaaggcg agcccggtca ggtcatccct ttttacgaat
     2401 atccggggtc ccttcagggc cttggtcaag ttgggcaatt cctgcagggg ggtcggcaga
     2461 ttggccagtc ggactctggg aagggtattt agtttcatcg attgtctcct ccgtatggat
     2521 aatagctaaa gctacagccc tgcaccaatt cgaatatgta ggtcgtcccc accgaattca
     2581 ggagtgattt cattatggta aacccccagc tctgccagtg gattattagc gttcgacttt
     2641 tccgggatgc ctcggttgtg gcccgggggc gaggagcttc tgggcaaaaa gcatcggccg
     2701 ggctggccct cttcggggag gcttcggccg gcttccgcag aatgcggcgg gctgaccggg
     2761 cgatcggccg gtcggcccta cgcttagaga acgccttcag gggggtttgg atggctagcc
     2821 cacccgatcc atgaaatgga agctgcctta tggtttttgc ccagaagctc cctgcccccg
     2881 ggcctggcac ccaatcgagg gccaccttta ggtggcgccc ttttcaatcc gcttgagcgg
     2941 ttcactgttt ttcatacctc cggctctgct ggaggtcatg attggtcggg catctccctg
     3001 gcaaattcgt tgatcaccct tttatccctt tttctttctt tcccttgaag cgacttccct
     3061 tgccagctgg gcgcaaaggc cggtgtaggt aa
//
LOCUS       DASPRR010000648         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1609785, whole genome shotgun sequence.
ACCESSION   DASPRR010000648 DASPRR010000000
VERSION     DASPRR010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609785"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1255
                     /locus_tag="VEL68_23670"
     CDS             53..1255
                     /locus_tag="VEL68_23670"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXZ39048.1"
                     /translation="MTHKERMLKAGRGEWADRLPWIPRIDLWHNSNSLRVTLPSKYRR
                     DATLDEIADDIGGGYHKLVPEFLKVRSPEDNVDRGIGVYRLRGMAYRPELTGVEREVK
                     KEGDTTFVTYHTPVGSVSSKILYTEEMKRAGASITWISEHVIKEPKDYRTVGYIFKNI
                     KVHPDFENYREYQEKVGDKGFAAAFANLSASPMHHIMKEFLDATQFYLEMFDHPKEMR
                     QLSEDLEPYFDQVFKVLVDSPAEVIFSGANYDEIITYPPFFKEHILPYIQRLADMAHA
                     KGKLVLCHCDGENKGLLDLIAHSGMDIAEAICPQPMTKVTISEVKKALQGKVTIFGGI
                     PSVALLEESMSNAEFERFMKNLFREIAPGDRFILGVSDTTPPDAKFERLLRITEMVQE
                     WGQLPMKF"
     gene            1389..2027
                     /locus_tag="VEL68_23675"
     CDS             1389..2027
                     /locus_tag="VEL68_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HXZ39049.1"
                     /translation="MVDQEKYFQALSAGKMDEVKALTQKALDAGDSPDKILKEGLIKA
                     MDRIGMLFKNNEIYIPEVLIAARAMHAGLGVLKPILAKSNVSAAAKIVIGTVKGDLHD
                     IGKNLVGMMLEGAGFEVIDIGIDAGPEKFVQAAKEHKANVIGMSALLTTTMMQMKGTV
                     EAVQAAGLKGKVKTMVGGAPVTDEFARQIGADGYAPDAASAVSRLKELLKIS"
     gene            2143..3114
                     /locus_tag="VEL68_23680"
     CDS             2143..3114
                     /locus_tag="VEL68_23680"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ39050.1"
                     /translation="MRNMLWLLAIGLMVLSPAQVQAQDFPKKPISLIVPYAAGGSTDV
                     LARALAKLSAKHFPVQVMVVNQAGGAGIPGRVAVVNAKPDGYTLLFGYGSGEDLVTPH
                     TMKIPYHQFKDLVPVCQISIVSLVLVVPSAHPAKSLKEFVDWAQKQNRQITGAVSTRG
                     ASVDITMQAIMKVAGVKAETIPFRGGAEAATAVLGGHTDFAGNTPSEVLPHVKAGRLR
                     ILAVCLRERDPIIPDIPTFMEQGFDVFTMGAIRGIGAPKGTPGPIVDYLESNFKKVTD
                     DPEFHSMAKEIFHPVMYRDRKVFLKNMTDGFEMYGQLIDKLGLKEQK"
     gene            3187..>3599
                     /locus_tag="VEL68_23685"
     CDS             3187..>3599
                     /locus_tag="VEL68_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39051.1"
                     /translation="MIPSSPENKIKRYLAFWNNSLVERPMVGFSLGGWFSFQSYSAIQ
                     KHRGAERLTLDMLSPERHFNDYDRIVSSFEEIEDDVIHSVAPIPAFPWLEAMLGIRAQ
                     VGNESIWAKEGGFDYRDIDKLNLSRDNPWRKKYLE"
BASE COUNT          883 a          914 c         1064 g          738 t
ORIGIN      
        1 aaatatcgga tcagggaaaa agataagaac aggcttagaa ggaaaagact tcatgaccca
       61 caaagaaagg atgctcaaag caggccgggg ggaatgggcg gaccggcttc cctggattcc
      121 ccgaatcgac ctctggcaca actcaaactc cctgagggta acccttcctt cgaagtatcg
      181 ccgcgacgcc acactggatg aaatcgcgga cgacattgga ggaggctacc acaagctggt
      241 ccctgaattt ttgaaagtgc gttcacccga agataatgtg gaccggggaa tcggggttta
      301 ccggctgagg ggaatggcct accggccgga actcacgggg gtggagaggg aagttaaaaa
      361 agaaggggat accacatttg tgacctatca tactcccgtt gggtcggttt ccagcaaaat
      421 cttgtacaca gaggagatga aaagggccgg ggcctccatc acctggatct cggagcacgt
      481 gatcaaagaa cccaaagatt acaggacggt gggttacatt ttcaaaaaca tcaaggtcca
      541 cccggatttt gagaattacc gagagtatca ggaaaaggtg ggagataagg gatttgctgc
      601 cgcattcgcc aacctatccg ccagccccat gcaccacatc atgaaggagt ttctggatgc
      661 cacccagttc tatctggaga tgttcgacca tcccaaggag atgcgccagc tcagcgagga
      721 cctggagccc tattttgatc aggtcttcaa ggttctggtg gattctcctg cggaagtcat
      781 tttctccggc gccaattacg acgagataat cacctatccc cctttcttta aagaacatat
      841 cctgccttat atccagaggc tcgccgacat ggctcatgcg aaggggaagc ttgtcctctg
      901 ccattgcgac ggggagaaca aagggctttt ggacctgatc gcccacagtg ggatggatat
      961 cgccgaagcg atctgcccgc agcccatgac caaggtgacg atcagcgagg tcaagaaggc
     1021 ccttcaagga aaggtgacca ttttcggagg gattccgtct gtagcccttc tggaagagag
     1081 catgtccaat gcggaatttg aacgttttat gaaaaatcta ttccgggaaa ttgccccggg
     1141 agaccgcttc atcctcggag taagcgacac cacgccgccg gatgccaaat ttgaaaggct
     1201 gctgcggatt acggagatgg tccaagaatg ggggcagctg cccatgaaat tttaggtttt
     1261 attttttggc ccctcaatgg tttaataaaa gagtcaaatt ttttccctca atttcccgcg
     1321 ggaaagaggg aggcaatccg gcaggtaggg ttaaacaaaa taaatgatcg caaaggagga
     1381 aaagagggat ggttgatcag gaaaagtact tccaggccct ttcggcgggg aagatggacg
     1441 aggtaaaggc tttgacccag aaggccctcg atgctgggga ttcgccggac aagatattga
     1501 aagaagggct catcaaggcc atggaccgga tcgggatgtt gttcaagaac aacgagattt
     1561 acattcccga agtgctcatc gccgcgcggg ccatgcatgc ggggctgggg gttctgaaac
     1621 ccattctggc taagtccaac gtctccgcgg cggccaagat cgtgatcggg acggtcaagg
     1681 gcgacctcca cgacatcggg aagaacctgg tggggatgat gctcgaggga gcagggtttg
     1741 aggtgatcga catcggtatc gatgcagggc cggaaaagtt tgtgcaggcg gccaaggagc
     1801 ataaggccaa tgtgatcggg atgtcggctc tgctcactac gaccatgatg cagatgaagg
     1861 gcacggtgga agccgtgcag gccgccgggc tgaaggggaa agtgaagacc atggtggggg
     1921 gcgccccggt gaccgacgag tttgcccggc agatcggggc cgacgggtat gccccggatg
     1981 cagcctcggc agtgagtcga ttgaaagagc ttctgaagat ctcctagaac ttcaatccga
     2041 taggagattg ccaaaggtgt tcagccaggg tagaaaagat tcgccttgcg tcggcgcaga
     2101 agaaggctca agaggaatca accaaccctg aggaggagga ctatgcgaaa catgctttgg
     2161 cttttggcca tcggtctgat ggtcctgtct cctgcccagg ttcaagccca ggattttccc
     2221 aaaaaaccga tcagcctgat cgttccctac gcggcagggg ggtcaaccga cgtactcgcc
     2281 agagcactgg ccaagctgtc ggctaagcat ttccccgtgc aggtcatggt ggtcaatcag
     2341 gcagggggag cgggaatccc cggccgggtg gccgtggtca acgccaagcc ggatggttat
     2401 accttgcttt tcgggtacgg gtccggagaa gacctagtca ctccccacac catgaagatt
     2461 ccctaccatc agttcaaaga cctggtcccg gtttgccaga tctccattgt ttccctggtc
     2521 ctcgtggttc cttcggcgca cccggccaag agcctgaaag agtttgtcga ttgggcccaa
     2581 aagcagaacc ggcagatcac cggcgcggtt tccacccggg gagcctcggt ggatatcacc
     2641 atgcaggcca tcatgaaggt ggccggggtg aaggcagaga cgattccctt ccggggcggg
     2701 gcggaagcgg cgaccgcggt cttgggcggg cacacggact tcgctggaaa caccccctct
     2761 gaggttctgc cccatgtaaa ggccggccga ttgcgaatct tggcggtttg tctgcgcgaa
     2821 cgggacccca tcattcccga tatccccacc ttcatggagc agggattcga cgtgttcacc
     2881 atgggtgcca tccgggggat tggggccccc aagggaacgc cgggtcctat cgtcgattat
     2941 ctagagtcca acttcaaaaa ggtgacggat gatccggagt ttcactccat ggccaaggaa
     3001 atcttccatc cggtcatgta ccgggaccga aaggtgtttc tcaagaacat gacggatggg
     3061 ttcgagatgt acggtcagct gattgataag ctcggcctga aagaacaaaa gtgaaagatt
     3121 ataggggcga ccgaccggtc gcccttacac gttttgggaa tcgatgagcc tttcctggga
     3181 gccgtcatga ttccgtcaag ccctgaaaat aaaatcaaac gataccttgc cttctggaac
     3241 aacagcctgg tggaacggcc catggtcggg ttttccctgg gagggtggtt ttcctttcag
     3301 agctacagcg ctatccagaa gcacagggga gctgagcgcc tgaccctgga catgctttcc
     3361 ccggagagac acttcaatga ctatgaccgc atcgtgtcct ccttcgagga aatcgaagac
     3421 gatgtcattc attcagtagc ccccatccca gcctttccct ggctggaggc catgctgggg
     3481 atccgggccc aggtgggcaa cgagtccatc tgggccaaag aagggggctt tgattatcgg
     3541 gacatagaca agctcaatct ctcgagggat aacccctggc ggaaaaaata tttggagtt
//
LOCUS       DASPRR010000649         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1290181, whole genome shotgun sequence.
ACCESSION   DASPRR010000649 DASPRR010000000
VERSION     DASPRR010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290181"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="VEL68_23690"
     CDS             complement(<1..1810)
                     /locus_tag="VEL68_23690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39052.1"
                     /translation="MPGTREEGKKVGLRFSERMSGYLTEGEVNFEQGEKLGEEHNTPL
                     SFDVTVHIEDVEDFTKLSGRRAQLTGTVSYPPLGKNLPIRDGVFGLFRPDPATAKRHM
                     TYSFGFTGDDGKEYFLSGYKEIYDDPKFDVLEDMTKLFTRVYRGNSEDGPIYGAGILH
                     FKMWSLPSMLFSFEVTGTHNPLAKIKAVSQFFSFCYGELRDTYLKKVSPFYYSNYENL
                     VLRGELASGGGRGQEFFFFSGIHDKDFPWGDEEVFWDLGLIIRRADGGWDRYALTDRT
                     AEGLRLDVEKGNYGYEGPLYQILDGYQLSRSELHKSSLPPGLRKIQARVEIRFDYEKY
                     APVDVPFAMNPDHRKLIPDEVWEKIREWLPQLENLGWHMIPHRVKVQQGTIRLQDPAG
                     SREYTLVNQKTTGEAEVALFSNIRWPKLYYNYFCALSPEANTLYVKVRSDVLRGNRKD
                     VVIDRIEQELGKMIGRVVTLDLQIDDQGEKVFKPAEGKSFQMVDENILELNNDHFPTG
                     VFQRRVVSLRDEKGDLYLALEEDMDTLNLGSINSDRVATAASFKDPDKFRALDKVLEA
                     TGFLRKLDAAWRKSGKRKEDFFILIKPNFMFMYSTKD"
     gene            2102..2371
                     /locus_tag="VEL68_23695"
     CDS             2102..2371
                     /locus_tag="VEL68_23695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39053.1"
                     /translation="MKAKAHLVLPLDILEEVDRIAGKRKRSLFIAAATREKLERERFL
                     KTLEETKGSWSDKNHPELRTIRDMEHYVREKRRSYRQRARGISHE"
     gene            2364..>2637
                     /locus_tag="VEL68_23700"
     CDS             2364..>2637
                     /locus_tag="VEL68_23700"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXZ39054.1"
                     /translation="MSRFLLDSDIIIWHLRGRLEVTEMLRDLQRAGLPACSALSVLEV
                     QLGVKKGEEEKTARFLDSLRVFDVTREIATVAARLIREGKPRGITLD"
BASE COUNT          568 a          746 c          721 g          602 t
ORIGIN      
        1 ggtctttggt ggagtacatg aacatgaagt taggcttgat gaggatgaag aaatcctctt
       61 ttctcttccc agatttccgc caggccgcat cgagcttccg aaggaatccc gtcgcctcca
      121 ggaccttgtc cagagccctg aatttgtccg ggtctttgaa ggaggctgcg gttgccaccc
      181 ggtccgagtt gatgctcccc aggttgaggg tgtccatgtc ttcttccaag gccaggtaga
      241 ggtcgccctt ttcatcccgc agggaaacaa cccgccgttg gaataccccc gtcggaaaat
      301 gatcgttgtt gagctccagg atgttctcgt ctaccatctg gaaggatttc ccctcggcgg
      361 gtttgaagac tttttccccc tgatcatcga tctgcaggtc cagggtgacc acgcggccga
      421 tcatcttccc gagttcctgc tcgatccggt cgatgaccac gtccttccgg tttccccgca
      481 ggacatcgga gcggaccttg acgtaaagag tattagcctc gggagacagg gcgcagaaat
      541 agttgtaata aagcttcggc cagcggatat tcgagaagag cgctacctcc gcttcccccg
      601 tcgtcttttg attcaccaga gtatattccc gggagccggc cgggtcctgg aggcggatcg
      661 ttccctgctg caccttcacc cggtgcggga tcatgtgcca tccgaggttc tccagctgcg
      721 gaagccactc ccttatcttt tcccaaacct cgtcggggat caatttccga tgatcggggt
      781 tcatggcgaa aggaacgtcc acgggggcat atttttcgta gtcgaaccgg atctccaccc
      841 gggcttgaat cttgcggagg ccgggaggaa gggaagactt gtgaagctcg ctccgcgaca
      901 actggtaccc gtccaggatc tggtagagag gcccctcgta cccgtagttc cccttctcca
      961 cgtctagccg cagcccttcc gccgtgcggt ccgtcagagc gtagcggtcc cagcccccgt
     1021 cggcccgccg gatgatcagg cctaggtccc agaatacttc ctcgtccccc caggggaagt
     1081 ccttgtcatg gatgcccgaa aagaaaaaga actcctgccc ccttcctccg ccggaagcta
     1141 gctccccgcg caggaccagg ttttcatagt tgctgtaata aaaggggctg accttcttga
     1201 ggtaggtgtc ccggagttcg ccgtagcaga aggagaagaa ctgcgatacg gccttgattt
     1261 tcgccagcgg gttatgggtg cccgtgactt caaaagaaaa cagcatggag ggcaggctcc
     1321 acatcttgaa atgaaggatt cccgccccgt aaataggccc gtcctctgaa ttcccccggt
     1381 aaacccgggt gaagagcttg gtcatgtcct ccaggacgtc gaatttcggg tcgtcgtaga
     1441 tctccttgta cccggaaagg aaatattctt ttccgtcgtc cccggtgaat ccgaaggaat
     1501 aggtcatgtg ccgcttggcc gtggccgggt cgggccggaa gagtccgaag actccgtcac
     1561 ggatgggaag gttctttccc aggggcgggt aggaaacggt gccggtcaat tgggctcttc
     1621 gcccggagag cttggtgaaa tcctccacat cttctatgtg aacggttaca tcaaaggaaa
     1681 ggggagtgtt gtgttcttcc cccagctttt ccccctgctc aaaattcacc tccccttcgg
     1741 tcaaataccc ggacatcctc tccgagaagc gcaggccgac cttcttgcct tcctctctcg
     1801 tccccggcat gataccctcc tttctcaaca gctccgccac tggtcggagc ctttaaggag
     1861 aaagaataaa acccgttctt ctggccatca tatacccgga cgattcctcc tgcaactcgc
     1921 ggttccctcc acccccgagc gactcaaatc ctttctcgca accctgaagg ggttttcttt
     1981 gaagcttggc cccgagacag gcatcatggc agcggttcca atccacatga atacttgaca
     2041 tggggccaca ggttactgta tattttatgt aacaaacaag ttacacagga gtggaacttt
     2101 tatgaaagcg aaggctcact tggtccttcc cctagatata ttggaggagg tcgaccgaat
     2161 cgccggcaag aggaagagaa gccttttcat tgccgcagcc acccgggaga agctggaaag
     2221 ggaacggttt ctgaaaaccc tggaggaaac caaaggatcc tggagcgaca agaatcatcc
     2281 tgaactgagg acgatcaggg acatggagca ttatgtccgc gagaagaggc ggtcttaccg
     2341 ccaacgggcg agaggaatct ctcatgagta gattcctttt ggattccgat atcatcatct
     2401 ggcatttgcg cggcaggctg gaggtcacgg agatgctcag agatttgcag cgggctggat
     2461 tgccagcatg ttcagccctc tccgttctcg aagttcagtt aggagtcaag aaaggagagg
     2521 aggaaaaaac cgcacgattc ttggactctt tgagagtttt cgacgttacc cgggaaatcg
     2581 ctaccgttgc ggcccggctc attcgcgagg gtaaacccag ggggataacc ctggatt
//
LOCUS       DASPRR010000650         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_1610989, whole genome shotgun sequence.
ACCESSION   DASPRR010000650 DASPRR010000000
VERSION     DASPRR010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610989"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1083)
                     /locus_tag="VEL68_23705"
     CDS             complement(163..1083)
                     /locus_tag="VEL68_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXZ39055.1"
                     /translation="MGKYVFKRILMLLPVLIGVSLISFSLLHMVPGDPAELLSGEDAN
                     PEFIKAVRKEYGLDQPLYIQYFQFISHVIRGDFGISIRNREPVINLLKERFLFTLQLS
                     FLSILIAAFIGLIAGVISSTRQYSLFDNMSMVGALFGISMPIFWFGLLLMLVFSVHLK
                     WFPAGGKGTLWHLILPAIALGAASSAVIARMTRASMLEVIRQDFIRTARANGLREAIV
                     IYKHALKNAMIPVITVFGLEFGYMLGGAVLTETVFSLPGVGRLMVEGIFQRDYPIVQG
                     SMLLVASTFVVVNLLTDIAYALFDPKIRYE"
     gene            complement(1231..>2604)
                     /locus_tag="VEL68_23710"
     CDS             complement(1231..>2604)
                     /locus_tag="VEL68_23710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HXZ39056.1"
                     /translation="MMYENLAKFDEKLKIVPGLAEKWEQSKDGLTWTFFLRKGVKFHD
                     GTTFNAEAVKVFVERMIGPEKPSRAGLYTPFVKSVEVVNDSTVKIVLKSPFAFFLNNI
                     AHSASGIISPTALKTLRKDIARKAVGTGPFKFVEWVHGDHLTMIRNDDYWGGKPYLDK
                     IVVKTVKEDSARVMMLQSGDAQLIVRIPPEDIPRLEKEKAVKLDTTETLRVLYIGINC
                     AKKPFTDVRVRQALNHAVDKEAIVKSIYQGRALVSNGIVAPLATGYFPVQGYSYDPDK
                     AKKLLAEAGFPNGFKAKLWTPQGRYPKDFEMAQAVQQQLKKVGVDCTLDTMEWAAYLA
                     ATRKPPQENQSELFLLGWAPSSAEARWILYPLCSTEQWVPQGNNRFFYSNKEFDGLVD
                     KFTLATTKAEMDKNLKAAQELLDKEAPAINILVTKETIGYSSKLKGVINSPLELTYFT
                     HQTYLEK"
BASE COUNT          585 a          706 c          742 g          571 t
ORIGIN      
        1 ttagtcaggt cgccttcatt cggatggtac ggagagagcc agggggccag gatggcggtg
       61 gccacaaaaa gaatgacgat gatccccccc acgatggcgg cccggttccg tttgagtctc
      121 ctccagacct cccgaacctg actggttttc cggcctctct catcactcat agcgaatctt
      181 ggggtcgaaa agggcgtagg caatgtcggt taataagttg accaccacaa aggtcgaggc
      241 caccaaaagc atggacccct gaacgattgg atagtcccgc tggaagatcc cctcgaccat
      301 caaccttccc actccgggga gggagaagac cgtctcggtc aaaaccgccc cgcccagcat
      361 gtagccgaac tccagaccga agacggtgat caccgggatc atggcattct tcagagcgtg
      421 cttgtaaatg acgatggcct ctctcagccc gttcgcccgc gccgtacgga tgaaatcctg
      481 gcggatcacc tccagcatgg aagcccgggt catgcgggcg atcactgccg aggaagccgc
      541 ccccagggcg atggccggaa ggatcaggtg ccagagggtc ccttttcccc ccgcgggaaa
      601 ccatttcaga tggacggaaa agaccagcat caggaggagg ccgaaccaga agatgggcat
      661 ggagatcccg aagagagcgc ccaccatact catattgtca aagagggagt actgccgggt
      721 cgaagagatg accccggcga tcagcccgat gaatgcggca atcagaatgg agagaaaaga
      781 aagctgaaga gtgaagagaa accgttcttt cagaaggttg atcaccggct cccggttgcg
      841 gatggatatg ccgaagtctc cccgtataac atgagatatg aattggaaat actggatgta
      901 aagcggctgg tccaacccgt actccttgcg gaccgccttg atgaattccg ggttggcatc
      961 ttcccccgac aataactccg ccgggtctcc aggaaccatg tgaagcaggg aaaaggagat
     1021 cagggaaacc ccgatgagga ccggcaggag catgaggatt ctcttgaaga cgtatttgcc
     1081 catcctttac agttccaaaa acgcatagcg cataaagctt ggcgccttgc gagagcagaa
     1141 aatagaattc ctttttggta aagccgcaaa aatgaaaggg cgactcggat tcagccgccc
     1201 tttcattctc aggtcaaccc gaatctctcc ttatttttca agataagtct ggtgggtgaa
     1261 ataggtgagt tccagggggg agttgatcac ccctttgagt ttgctgctgt acccgatggt
     1321 ctctttggtg accaggatgt tgatcgcagg agcctccttg tccagcagtt cttgggcggc
     1381 cttcaaattc ttatccatct ccgccttggt ggtggccagg gtgaatttgt ccaccagccc
     1441 gtcgaactcc ttgttggagt agaagaagcg attgttcccc tggggtaccc actgttccgt
     1501 agagcacagg ggatagagaa tccaccgggc ctcggccgac gaaggagccc atcccaggag
     1561 gaagagttcg gattggtttt cctgcggggg tttgcgggtg gcagcgaggt aggccgccca
     1621 ctccatggta tccagcgtgc agtccacccc cacctttttc aactgctgct gcacggcctg
     1681 ggccatctca aagtctttgg gatagcgccc ctgcggggtc cacagcttgg ctttgaaacc
     1741 attggggaac ccggcctcgg ccaacaattt cttggcttta tcgggatcat aggaatatcc
     1801 ttgaaccgga aaatatcccg tagcaagagg ggcgacgatc ccgttgctca ccagggccct
     1861 gccctggtag atggacttga cgatggcttc cttgtctacg gcgtggttca gggcctggcg
     1921 gacgcggaca tcggtgaagg gctttttggc gcagttgatc ccgatataaa ggacgcgcaa
     1981 agtttcggtc gtgtcgagtt ttaccgcttt ctccttctcc agccgaggga tatcctcggg
     2041 ggggatgcgg acgatcaatt gggcgtcacc ggattggagc atcatgaccc gggcgctgtc
     2101 ttctttgacc gtcttcacga cgatcttatc caggtagggt tttcctcccc aataatcatc
     2161 gttcctgatc atggtcaggt ggtccccatg aacccattcc acgaatttga aggggccggt
     2221 tcccacggcc ttgcgggcga tatctttccg aagggttttc aaggccgtgg ggctgatgat
     2281 acccgaggca ctgtgggcta tattattgag aaagaaggcg aaaggggact tcaggacgat
     2341 cttgaccgtg gaatcattga ccacttccac ggacttgacg aatggagtgt agaggcccgc
     2401 ccgggagggc ttttccggcc cgatcatccg ttccacgaaa acctttaccg cctcggcgtt
     2461 gaaagtagtt ccgtcatgga acttgacccc tttgcggagg aagaaggtcc aggtgagccc
     2521 gtctttggat tgttcccact tctccgccag gccgggaacg atcttcagct tctcatcaaa
     2581 cttcgccagg ttttcataca tcat
//
LOCUS       DASPRR010000651         6443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_313996, whole genome shotgun sequence.
ACCESSION   DASPRR010000651 DASPRR010000000
VERSION     DASPRR010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313996"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VEL68_23715"
     CDS             <1..847
                     /locus_tag="VEL68_23715"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HXZ39057.1"
                     /translation="GEKIAQLKEQISAAAGEHFRKGVSYFRTHSYNSARKEFLLTLYL
                     KPDHVEAIQYLKEKLVGEDFLTYEVKRGDTIKDVARKIYKDPQKDFVIAYFNGLKIDG
                     RIEPPMILKMPFLEIAPVKTTSIRPQRATEPAPEIALEIKEILEEARTTYEKGDYPES
                     AALAEKVLKYDPANKEIHELMNASYYEWGKRLSGERKYQEALEAFHRVDPGYKDVNFQ
                     LAHNRKQLAEEHYLRGVKFFISEHVKGAIQEWETTLSLEPDHPRAKKDIENARELLQK
                     LEKIK"
     gene            complement(1029..2012)
                     /locus_tag="VEL68_23720"
     CDS             complement(1029..2012)
                     /locus_tag="VEL68_23720"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ39058.1"
                     /translation="MTAAGRYRKLWVIVGLIGASVISFPGMAPGQAYPTKPITVYCGY
                     SPGATTDLTARALAGAAEKFLGVPVVVENKAGGSATVCAALVASKPADGYTLGILDMA
                     ALTVRPHLLKVAFNPLTDFTLVLQYGLYVGGLCVLSESPIKTIDEFIAHAKANPGLAY
                     GASGQFSRGHLAMELFAKCKGLTFKHVPFKGGADADTALLGKHLDFVGGSGGHIPFVK
                     QGVFRILMLFSMEKRDPHFPGIPMQKELGCEDVPPNSYILIAPKGLADPVFQKAHEAF
                     KKASEQPDFLKIMEANNLPYEYKSRAQLEKDVPAQFEWYMNILQKWGIGKK"
     gene            2814..3704
                     /locus_tag="VEL68_23725"
     CDS             2814..3704
                     /locus_tag="VEL68_23725"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXZ39059.1"
                     /translation="MKTKEFAGVFPYLVSPVNSQGEVMADVLARLVEHLIQNGVHGLT
                     PLGSTGEFAYLTWDQRRRVVEVILDASKGKVPVVAGVAHTSIREAARQAREMEAIGVD
                     GILAIMDTYFPVPAEGVAAYFRAVAEAVSCPIVLYTNPSFSGTNLSFEVIESLLSTPN
                     IRYLKDASANTGNLLTLMNRLGDRVRVFSASAHIPLSVMMLGGVGWMSGPACVIPRQS
                     VRLYELSCRRQWDEAIALQKRLWSINWIFQKYALAPCIKACLEIQGFPVGPPIPPLQP
                     LKGAALQEIEGVLKSLQALP"
     gene            3795..4235
                     /locus_tag="VEL68_23730"
     CDS             3795..4235
                     /locus_tag="VEL68_23730"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXZ39060.1"
                     /translation="MTIDRISGTFFILLGLFVIWEDRVLPLGTTGNPGPGYFPLLLAV
                     LLIFMGGILAIRGGQAIALRSIKWPEARTAMAILGCCIFATLGMETIGYRVTMVLILS
                     FLFGILERLKMWQVLALTLSLAIGSFWLFDTFLKVPLPRGGFGF"
     gene            4235..5758
                     /locus_tag="VEL68_23735"
     CDS             4235..5758
                     /locus_tag="VEL68_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXZ39061.1"
                     /translation="METTLQNLLLGFSTVFTATNLFYSLIGCLIGTMVGVLPGVGPLA
                     GISMLLPTTFGLSPASAIILLAGIYYGAMYGGSTTSILMRIPGEAASVITCIDGYAMA
                     QKGRAGPALAIAAIGSYIAGTLSVVGLMFLAPPLAKFALGFGPPEYCSLLLCGLVVLG
                     YMTGGSVVKNLSMIVLGLLFGMIGMDQMTGYFRFSYGIVALGDGIGIVPVAVGLFGIS
                     EILATAGQTKRPEVIKPRFRDLFPSRTELRDSMGPIGRGTVMGFLIGIIPGSAHIIST
                     FVSYAVERRISKHPERFGKGAIEGVAGPESANNAASEGALVPMLALGVPTGPIPAVLL
                     AALMVHGINPGPLFIKEQPVIFWGLIASMYVGNLVLLLLNLPLVSIFVNFLRVPYQIL
                     YPTILMFCVVGVYAVNSSLVDVGIMSIMGLLGYVLRKLDFETAPMVLGVILAPIIEVS
                     FRQALAMSNGEYSIFVQRPISLTFLAGALLMILLALKPLIMKKKDWRERLTEAEKSG"
     gene            5809..>6443
                     /locus_tag="VEL68_23740"
     CDS             5809..>6443
                     /locus_tag="VEL68_23740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXZ39062.1"
                     /translation="MKKTKAHLLLVIASCAGMIGLLSGMAGADEPYPTRPIQVFVPFP
                     PGGVADLVARPFATALEKHVKQPVVVVNKTGAGGAVGMQAAAVAKPDGYTLMVALSSI
                     SVMPEVDALFGRPSTYKLKDFAPIALLTADPTIFVVKKEAHWKTVADFVADAKKRPNE
                     IKYASSGVYGTMHVAMEMFAHAAGIKLRHIPTAGGGPALTALLGGHVDTISG"
BASE COUNT         1442 a         1741 c         1776 g         1484 t
ORIGIN      
        1 cggggaaaaa atcgcccagc tgaaggagca aatttccgct gccgcgggcg agcatttcag
       61 gaagggcgtg tcttatttca gaacccattc ttacaactca gcgcggaaag agtttctcct
      121 cactctctac ctaaagccgg atcatgtgga agcgatccaa tatttaaaag aaaaattggt
      181 cggcgaagac ttcctcacct atgaggtcaa gagaggagac acaatcaaag atgtggcccg
      241 gaaaatctat aaagacccgc aaaaggattt cgtcatcgcc tatttcaatg gcctgaaaat
      301 cgatgggcgc attgagccgc ccatgatcct gaaaatgcca tttctggaaa ttgccccagt
      361 gaagaccact tccattcgcc cccaaagggc aacggagcca gcccccgaga ttgctttaga
      421 aataaaagag atcctggaag aggcccggac tacttacgaa aagggggatt atccggaaag
      481 cgcggctctg gcggaaaagg tcttgaaata cgatccggcg aacaaggaaa tccatgaatt
      541 aatgaatgcc tcttattacg agtgggggaa acgactcagc ggggaaagga aataccagga
      601 agccctggaa gcgttccaca gggtggaccc cggatataaa gatgtcaatt tccagcttgc
      661 ccacaaccgg aaacaattgg cagaagaaca ttacttgaga ggggtgaaat tctttatttc
      721 cgagcatgtc aagggcgcga ttcaagaatg ggaaactacc ctttccctgg aacccgatca
      781 ccccagggcg aaaaaggaca tcgaaaacgc ccgcgaactt cttcaaaaac tcgaaaagat
      841 caaatgatcc ttttggacaa tggtatttgg ggacaaccgc tctatagatg gttttcggga
      901 aatacctttt tctccatctt taacctgata aacaacagtt agaaaaaccc ccttatccgc
      961 cttttttctt aatctaacaa aaccgattca accagagcat ggagcgaggg agggctcctt
     1021 caagaaggct attttttgcc aatcccccat ttctgaagga tgttcatata ccattcgaat
     1081 tgagcgggga catctttttc caattgcgcc cggctcttat attcataggg aagattattc
     1141 gcctccatga tcttcagaaa atcgggctgc tcgctggcct tcttgaaggc ctcatgcgcc
     1201 ttttggaaaa caggatcggc aagacccttg ggggcgatta ggatgtaact attcggcggt
     1261 acatcctcgc accccaattc tttctgcatg gggatgccgg gaaaatgagg gtcccgcttt
     1321 tccatactga acaacatcaa aatgcggaat accccctgct tcacgaaagg aatatgcccg
     1381 ccggaccccc cgacaaaatc cagatgcttg cccaagagag ccgtatccgc atccgcccct
     1441 cccttgaagg gcacatgctt gaaagtcagg cctttgcatt tggcaaagag ctccatggcc
     1501 aagtgtccgc ggctgaactg ccccgatgcg ccgtaggcga ggcccgggtt ggctttggca
     1561 tgagcgatga actcatcgat ggttttgatg ggggattcgg agagaacgca gagccctcct
     1621 acgtagaggc cgtactgaag aaccagggta aaatccgtaa gtggattaaa ggcaaccttg
     1681 agaaggtggg gacggacagt caaggcagcc atgtcgagga ttcccagcgt atacccgtcc
     1741 gcgggcttgc tggccaccag ggctgcgcac accgttgcgg agccgcccgc tttgttttcc
     1801 acgaccaccg gaacgccgag gaatttttcc gcagcgccgg ccagggcgcg ggcggtcagg
     1861 tccgtcgtcg cccccggcga gtacccgcag taaaccgtga tgggcttggt cgggtacgcc
     1921 tggcctggcg ccatgcccgg gaaagaaatc accgaagccc cgatcagccc aacaatgacc
     1981 cacagcttcc gataccttcc tgctgctgtc ataaaacacc tctatgggat gagattcgtc
     2041 ttgaggaaat cgataagagg ctactagaag ctggtaaaag ttcttttccc accgggggac
     2101 gtcataaatg agcggattga attcccaatg tcccccaaaa agaatccttt gctttgctca
     2161 tccttctcta tattatctcc atccttttgt caatccccga taaggccttg tgtgttagca
     2221 aaatagaaat gtcccccccc gataaggcct tgcccagccc cagttcttaa aaaagagtcc
     2281 ttcgaaaaaa agatctctgg aaaggagggg ggatctcatg gggttcaggg cgaagaaatt
     2341 tggatcatca tcctgatgcg gtggctcttc cattggatcg ggcctgtcct ggggggattg
     2401 aaagggaaac atgctttggc tctcttttct gcttggagga ttctcctctc aggcctctgc
     2461 ctgcggaaca tggggaggag tttcatggaa ggataagcac ccgccgagcc tgatgatccc
     2521 aattcctcca ccctgaaccc caacaagcac gaaaaactgg ggtgggcaag ccgataaggc
     2581 tccatgcgga tctttcccca cgccaggccc ctccggggtt attgaattga aatcccttgg
     2641 ggctgaattg acattgccgg gaaggtccaa tatgatgacc taagccatta gttgattcag
     2701 aaaaaaataa atggaagaac catgcgggaa tatccatccc cttgccggcg atcaggccaa
     2761 agcatttggc tccgcacgag gtggatgggc ctccctcagg aatgggaatg aagatgaaga
     2821 cgaaagaatt tgccggagtc ttcccctacc tggtatcgcc cgtaaattcc cagggggaag
     2881 tgatggcgga tgttcttgcg cgcctcgtgg aacacctcat tcagaatggg gtccacgggt
     2941 tgaccccctt gggaagcacc ggggaattcg cctatctgac ttgggaccag aggcggaggg
     3001 tcgtggaagt gatattggat gcctctaagg gaaaggtccc ggtggtggcg ggtgtggccc
     3061 acacctctat ccgggaagcg gctaggcagg ccagggaaat ggaagccatc ggggtggatg
     3121 gcatcctggc tatcatggac acctattttc ccgttcctgc cgagggagtc gccgcctatt
     3181 ttcgcgccgt tgctgaggcc gtctcctgcc caattgttct gtataccaat ccaagcttct
     3241 ccggcacaaa tctgtccttc gaggtcatcg agtccctgct ctccaccccc aatatccgtt
     3301 acctgaagga tgcttcggcc aacacgggaa atctccttac cctcatgaac cggctggggg
     3361 accgcgtaag ggtcttcagc gcttcggcgc acattcccct gtcggtgatg atgctcggag
     3421 gggtggggtg gatgtccggc cccgcctgcg tgatcccgcg gcaaagcgta aggctatatg
     3481 aactttcctg ccgccggcag tgggacgagg cgatcgcttt gcagaagcgg ctctggagca
     3541 ttaactggat tttccagaag tatgccctgg ccccctgcat caaggcctgc ctggaaattc
     3601 agggattccc cgtggggccg cccattcctc cgctccagcc gctgaaaggc gcggccctcc
     3661 aggaaatcga gggggtcttg aagtccctcc aggcccttcc ttgaagaccc gggctttcga
     3721 aaaaaatccc tccgacagag gaaagatatg ctacttttgt tctaaaggaa aaattcgggt
     3781 aattggatgc gaatatgacc attgatcgta taagtgggac cttttttatt cttcttggcc
     3841 tctttgtcat ctgggaggat agagttttac ccctcggcac caccgggaac cccggtcccg
     3901 gctattttcc cctgctgctg gccgtcctgt tgattttcat gggaggcatt ctcgctatcc
     3961 gagggggcca ggccatcgct ctgcgctcca tcaaatggcc ggaggcgcga accgcaatgg
     4021 ccattttggg atgctgtatc tttgccactc tgggaatgga aaccatcggg tatcgggtta
     4081 ccatggtcct catcttgagc tttttgttcg gtatcttgga acgcctcaag atgtggcaag
     4141 ttctcgccct gaccctatcg ctggccatcg ggtccttctg gctttttgac actttcttga
     4201 aagtgccgct tccccgcgga ggttttggat tttaatggaa accaccttgc aaaacctatt
     4261 actaggcttc tcgactgttt tcacagcgac gaatttattc tactccctga tcggctgtct
     4321 gatcgggacg atggtggggg tgctgccggg agtcgggccc ttggcgggaa tcagcatgct
     4381 ccttcccacg acctttggct tgagtccggc ctccgcgatc atccttttgg ccgggatcta
     4441 ttacggggcg atgtacgggg gatctaccac ctcgatcctc atgcgcatcc ccggggaagc
     4501 cgcttcggta attacctgca tcgacggcta tgccatggcg cagaaggggc gggccggacc
     4561 ggcattggcc attgccgcca tcgggtccta cattgccgga acgctgagtg tggtcggtct
     4621 catgttcctg gccccgcccc tggccaaatt tgccctcggt ttcggccctc cggagtattg
     4681 cagcctcctc ctctgcgggc tggtggttct cggctacatg accggggggt cggtggttaa
     4741 gaacctgtcc atgatcgtcc tgggattgct cttcgggatg atcggcatgg atcaaatgac
     4801 gggttacttc cgattttctt acggcattgt ggccctgggg gatggaatcg gtatcgtacc
     4861 agtggccgtt gggctattcg ggatctccga gattctagcc accgcgggcc agacgaaaag
     4921 accggaagta atcaagcccc ggttccgaga cctcttccct tcgcgtacgg aattgagaga
     4981 ttccatgggg cctatcggcc gaggaaccgt catgggattt ctgatcggca tcattcccgg
     5041 gtcggcccat atcatttcca cttttgtctc ttatgcggtg gagcgcagga tctccaagca
     5101 cccggaacgg tttggaaaag gggctattga aggcgtggca gggccggaat cggctaacaa
     5161 cgcggccagt gagggagccc tcgttcccat gctggcgttg ggagttccga cgggccccat
     5221 cccggcggtt ctcctggctg ccctaatggt tcacgggatc aatcccgggc cgctgttcat
     5281 caaggaacag ccggtgatct tctggggcct cattgccagc atgtatgtag ggaatctggt
     5341 tcttcttctg ttgaaccttc ctctggtgag tatctttgtc aattttttac gtgttcccta
     5401 ccagatcttg tatcccacca tcctgatgtt ttgcgtggtg ggggtctacg cggtgaactc
     5461 cagccttgta gacgtgggca tcatgagtat tatgggactc ctgggatatg tcttgcggaa
     5521 gctggacttt gagacggcgc ccatggttct gggagtgatc ctggccccca ttatcgaagt
     5581 gagtttcagg caggccctgg ccatgtccaa cggggaatat tccattttcg ttcaacggcc
     5641 gatctcgctc acctttctcg cgggagcatt gctcatgatt cttctcgcct tgaagccttt
     5701 gatcatgaag aaaaaagact ggcgggagcg tttgacggag gcggaaaaga gcggttaatg
     5761 gcccccagaa tagccggaga aagatttaaa tcaaagagga ggaggaggat gaaaaaaaca
     5821 aaagcccatt tattgctggt gatcgcctca tgcgctggaa tgatcggcct gctttcggga
     5881 atggccgggg cggatgaacc ttaccctacc cgcccgatcc aggtcttcgt cccctttcca
     5941 ccaggcgggg tggccgacct ggtggccagg ccttttgcca cggcccttga aaagcacgtc
     6001 aaacagccgg tcgtggtggt caataagacc ggcgccggcg gggccgtggg catgcaggcc
     6061 gcggccgtgg ccaaaccgga cggctatacc ctgatggtgg ccctttcgag catttccgtg
     6121 atgcccgaag tggacgccct attcgggcgc ccttcgacct ataaactgaa agacttcgcg
     6181 cccatcgccc ttttgaccgc ggacccaacg atcttcgtgg tcaaaaagga agcccactgg
     6241 aaaaccgtgg cggatttcgt cgccgacgcc aaaaagcggc ccaatgaaat caagtacgct
     6301 tcatccggag tgtatggaac catgcatgta gccatggaga tgtttgccca tgccgcaggt
     6361 attaaattaa ggcatattcc cacggctgga ggaggaccgg ccctgaccgc tcttctgggg
     6421 ggccatgtgg acaccatatc agg
//
LOCUS       DASPRR010000652         5563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_314626, whole genome shotgun sequence.
ACCESSION   DASPRR010000652 DASPRR010000000
VERSION     DASPRR010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314626"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VEL68_23745"
     CDS             <1..184
                     /locus_tag="VEL68_23745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39063.1"
                     /translation="GEHWKNGLLVGLGPRFRSGEVRTEIHTQDMAPTILEFFGVQTPP
                     SNEGKSVLSLLGEVLN"
     gene            complement(336..1394)
                     /locus_tag="VEL68_23750"
     CDS             complement(336..1394)
                     /locus_tag="VEL68_23750"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HXZ39064.1"
                     /translation="MPTLKGEQLEKICFHLLCAAGTPEDHSRIVAQHLAENNLAGHDS
                     HGFIRIIQYIRQIKEGVIVPTAKPEIVSDSPVIAQVDGHYGFGQVAARFSTELAINKA
                     KKQGVGCVAVRRLRHLGRLGAWAELAAKAGCAAILFTSTGGQALICSPFGGSKRRLGT
                     NPIAMAFPGENEEVIVADFATSAAAEGKVRVYRARGHRLPDGWILDKEGRPSNNPDDF
                     YDGGAILPVGGSVGHKGYCLAFMTDLFGNLLSRDGFPPKPGDQNLSNASLILVLDVKR
                     FAPLQTIKSEISMMVDYVKETPLAQGFDQILYPGEKEAKSRKARNKSGVEIEDDTWNQ
                     VMALVKEYNLADKIGKLP"
     gene            complement(1520..3739)
                     /locus_tag="VEL68_23755"
     CDS             complement(1520..3739)
                     /locus_tag="VEL68_23755"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXZ39065.1"
                     /translation="MPGYSMTLPSHNSGKRRKAIDLTLGLGITAGVAFLFFLRLGVFE
                     SAHYKLYDLSLQARGNFQAPSEMVIVAIDDATVSSLGRWPWPRSKVAELIARLSQAGA
                     RTIAVDAVFLPADAEQASGNDRILGEAVQKAGNVLLPFYFTLGKSKEQKKKGEIPAPV
                     LSSAFLLFDDPKKFSDFPPPAGEEIFFSVPEIIGGARAMGHINFLPDVDGKVRWDPLI
                     IQYNGQYYPAFSLQVAAAAMGLTRGELKVNVGQLIRLGRTAIPTDRQGKMLISYYGGA
                     QSIPYLSCADIFSGKVLADSFRDKIVLVGVTAAGTHDFLATPFTHQFFGVEKHAHAVA
                     SILQGRFISRPSWMSFVEFGLVLLIGSLLTFLLPGRRPVIQLAFCLASLVGLTALMLG
                     AMRQGTWIQIFFPAVLVIFQYLLATVRRGPAVEREMQAGVQATSVMTREPREPALSEG
                     TLRLEAGGALKEIDRYQVLGELGHGAMGVVYKGRDPIIDRLVAIKTIRFDRLYEEKEI
                     QGLKDRFFKEAQAAGKLAHPNIVTIFDVGEYRGLSYMAMEYVEGDVLSRFGSKGQLLQ
                     VEEVLEIIANAAEALDFAHQRKIIHRDIKPANIMRTSEGQIKVMDFGIAKLPSSTLTQ
                     DGSVLGTPAYMSPEQIQGKDLDGRSDLFSLGSILYELLTGVKPFQGENLAALTYQITH
                     ENPPPASQLNPLVPSAVDEVLRKVLAKNPNERFSRGKEFAQALRNVLQDMKKTPPQA"
     gene            complement(3763..4854)
                     /locus_tag="VEL68_23760"
     CDS             complement(3763..4854)
                     /locus_tag="VEL68_23760"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXZ39066.1"
                     /translation="MKPFSPERYLRFFLILLVLSPLAGVIFAEDSLKFQVRKGDTLSY
                     LSFKTYGMFDSQIAEILKKENPQIKDIDLIYAGQQLIFPPPEEMNRRLADKIGKPAKP
                     EPEKETKEAPPPQPPTLSLPKVSETQVRAQKGVITFLEGQVEVKRSGEGQWGPARANM
                     ILTENDLIRVLSQSRAELILDNQSVLRLSENTVLTLQKMEEEKAAQKENTRMEMSMGR
                     LWVRVSKLFNPSSRHDVKTPTAIAGVQGTTYQLWVSGNQSTTIQVFEGAVNVYNPFPK
                     ALPPVPGAPSQVEKPREIAGPQEVRGPSPVSREEWTQIVLHQYQQITVTGRETPQPMA
                     FNPGRERQSDWVRWNEERDADFKPPARPL"
     gene            complement(4985..>5563)
                     /locus_tag="VEL68_23765"
     CDS             complement(4985..>5563)
                     /locus_tag="VEL68_23765"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXZ39067.1"
                     /translation="WAFGLSFGVNCLSCAFLPFKKEGPPPRTEPAPTLQNPKPPTGEK
                     EPVPLKTREPVPSVQKAALSSVAPAPAQHEAPPPPQVKAEPKPRETHIAHTVKWSGET
                     LSIISLWYTGNQGNWKTIAQTNTNLDPNRIYVGNEILIPVGLVKTQNSLPKEFVDRFY
                     SKAKKEKSKPAPSSGQTQEEEPKLFGPKKSSK"
BASE COUNT         1186 a         1516 c         1562 g         1299 t
ORIGIN      
        1 aggtgagcac tggaaaaacg gcctgctggt ggggctgggt cctcggtttc ggtcaggcga
       61 agtccgcaca gaaatccaca cccaggacat ggctccgacc atcctggagt tcttcggggt
      121 acagactcca ccgtccaatg agggcaagag cgttctctcc ctcctgggcg aggtattgaa
      181 ttgatttgct aatgtctgta tagccaaata taggtcagag caggcgttcc tggttcccgc
      241 cttccttacg gtttgttctt ttgtcgttac acctcgatct ttgaatgtcg ttccttgagc
      301 gttctgcctt gatctttgtt ccttggttgt tgtgtctaag gaagtttgcc aattttgtcc
      361 gccaggttgt actccttcac cagggccatc acctggttcc aggtatcgtc ctcgatctcg
      421 acaccacttt tattccgtgc ctttcggctt ttggcttcct tttcccccgg gtacagaatt
      481 tggtcaaacc cctgggccag cggggtttcc ttcacgtaat ccaccatcat ggaaatctcg
      541 gacttgatgg tctgcaaggg cgcgaacctc ttgacatcca ggaccaaaat gagagaggca
      601 ttactgaggt tctgatcgcc gggcttgggg ggaaaaccgt cgcgggacag gaggttgcca
      661 aagagatcgg tcataaaagc caggcaatac cccttgtggc ccaccgagcc ccccactggc
      721 aggatggcgc ccccatcgta aaaatcatcg ggattgttac tgggacggcc ctccttgtcc
      781 aggatccagc cgtcggggag tctgtgcccg cgggcccgat acacgcggac cttgccctca
      841 gccgcggcgc tggtggcgaa gtccgcgaca atcacctctt cgttttcacc ggggaaagcc
      901 atggctatgg ggttggttcc cagtcttcgc ttggagccgc cgaaggggga gcatatgagg
      961 gcctgcccgc cggtggaggt gaacagaatc gccgcacagc ctgcctttgc cgccaactcc
     1021 gcccaggctc ccagacgtcc cagatgacgc agccgccgca cggcgacaca gcctacgcct
     1081 tgtttcttcg ccttgttgat ggccagttcg gtggaaaacc tggccgccac ctgcccgaaa
     1141 ccgtaatgcc catccacttg ggcgatcact ggggaatcgc ttacaatctc cggcttggcc
     1201 gtcgggacga tcaccccttc tttgatctgg cggatgtatt ggatgatgcg aataaacccg
     1261 tgcgaatcgt gtcctgccag gttgttctcg gccagatgct gcgccacgat ccggctatga
     1321 tcctccggag tccctgcggc gcagagaaga tgaaaacaga tcttttccag ttgctcacct
     1381 ttgagcgtcg gcattcatga tcctcctttt cttatggttt ccctgtaaga atatatcctt
     1441 attaagattg gggcacaatc tcaaaagaag gcaagggcga agagtctgct tcaaaaggct
     1501 cctcgccacc ctttgccggt caagcctgtg gaggagtctt tttcatgtcc tgaagtacat
     1561 ttcgcaaggc ttgggcaaat tctttccctc tggaaaaacg ttcattggga ttcttagcca
     1621 agaccttacg gaggacctcg tccacggccg agggcaccag aggattcaac tgggaggctg
     1681 gtgggggatt ctcatgagtg atctgatagg tcagggcggc aaggttttct ccttgaaagg
     1741 gcttgacccc ggtcaagagt tcataaagga tcgagccaag ggaaaagagg tcggagcgtc
     1801 cgtccaggtc ttttccctga atttgctccg gggacatata agcaggggtc cccaaaaccg
     1861 agccgtcctg ggtgagggtg gaggagggaa gcttggcaat gccgaaatcc ataactttga
     1921 tctgcccctc tgaggtccgc ataatgttgg ccggcttgat gtcccggtgg atgatcttgc
     1981 gctggtgggc gaagtccagg gcttcggcgg cattggcgat gatctccaat acctcttcca
     2041 cctggagaag ttgtcctttg gagccaaacc ggctaaggac gtctccctcc acatattcca
     2101 tggccatgta ggagagcccg cggtattccc ccacatcgaa gatggtgacg atgttaggat
     2161 gggctagttt ccccgctgct tgggcctctt tgaaaaagcg atccttcagg ccctgaatct
     2221 ccttttcctc atagagccgg tcgaaacgga tggttttgat ggccaccagc cgatcgatga
     2281 tcgggtctcg ccccttatac accactccca tggccccatg gcccagttct cccaggacct
     2341 gataacggtc gatttccttc agcgcgcccc ccgcttcgag gcgcagcgtt ccctcggaca
     2401 gggcgggctc tctgggctcc cgggtcatga ccgaagttgc ttgaaccccc gcctgcattt
     2461 ctctttctac cgctggaccc cgccggaccg tggccagaag gtattgaaaa atcaccagca
     2521 cggcgggaaa gaaaatttga atccaggttc cctgacgcat agccccaagc attaaagccg
     2581 tcagaccgac caggctggcg aggcagaaag cgagctgaat aacaggccgc ctaccgggca
     2641 ggagaaaagt caagagagat ccgatcaaca ggaccagacc gaattccacg aaagacatcc
     2701 aagaggggcg ggaaataaag cgaccttgaa ggatactggc cacggcgtgg gcatgttttt
     2761 caactccaaa aaactgatga gtaaagggag ttgccagaaa gtcatgggtt cccgctgccg
     2821 tcacccccac caggactatc ttgtccctaa aggagtccgc caggaccttc cctgagaaga
     2881 tatcggcgca ggatagatag ggaatactct gggccccccc gtaataggaa atcagcattt
     2941 tcccctggcg atccgtggga atggccgttc ttcccaggcg aatcaactgg cccacattga
     3001 ccttcagttc cccccgggtc aagcccatgg ccgcagcggc cacctggagg ctgaaggcgg
     3061 ggtagtattg gccgttatac tggatgatca gcgggtccca gcggactttc ccgtccacat
     3121 caggaaggaa gttgatatgg cccatggccc gggctccccc aatgatctcc gggacggaga
     3181 aaaaaatttc ttcccctgcc ggagggggga aatccgagaa ttttttggga tcatcgaaga
     3241 ggagaaaagc agaggataga accggggctg gaatttcccc ctttttcttt tgttcctttg
     3301 attttcccag ggtgaaatag aaaggaagaa gaacatttcc ggccttctgc accgcttcgc
     3361 ccaggatccg gtcgtttcct gaggcctgct cggcatcggc ggggagaaaa accgcatcca
     3421 cagcaatggt tctggctcct gcctgagaca agcgggcgat caactcggcg actttagagc
     3481 gcggccaggg ccaccttccc aggctggaca cagtggcgtc atcgatggct acgataacca
     3541 tctctgaagg ggcttgaaag tttccccggg cctggaggga aaggtcataa agtttgtagt
     3601 gagcggattc aaagaccccc aggcgaagaa agaaaaggaa agcgaccccg gcggtaatac
     3661 ccagccccag ggttagatcg atggctttcc ttcttttccc tgagttatga gacggtaggg
     3721 tcatggaata cccgggcatt tcccgtaggc agtcttccct ttctaaagcg gtcttgccgg
     3781 gggtttgaag tccgcatctc tttcttcatt ccaccgaacc caatcgctct gacgctcccg
     3841 gcccggatta aaggccatgg gctggggggt ctcccggccg gttacggtaa tctgctggta
     3901 ctgatggagc acaatctggg tccattcttc gcgggatacg gggctcggtc cccgcacctc
     3961 ctgcgggccc gctatttctc tgggtttttc cacctggctc ggcgccccgg gaacgggggg
     4021 aagggctttg ggaaaggggt tgtacacatt cacggccccc tcaaaaacct ggatcgtcgt
     4081 agactggttc ccggaaaccc agagctgata ggtagttccc tggactccgg caatggccgt
     4141 aggggttttt acgtcatgcc gggaagacgg gttgaagagc ttggaaactc taacccatag
     4201 gcgtcccatg gacatttcca tccgggtatt ctctttctgt gccgcctttt cttcctccat
     4261 cttctggagg gtcagcacag tattttccga aagacgcagg acgctttgat tatccaggat
     4321 caactccgcc cgggattggc tcaggactcg gatcagatca ttttcggtca gaatcatgtt
     4381 ggcgcgggcc ggaccccatt gcccttctcc agatctttta acctccactt gtccttccaa
     4441 aaaggtgata acccctttct gggcccggac ctgggtttcg ctcaccttcg gcagggacaa
     4501 tgtcgggggc tgaggcggag gggcctcttt ggtttctttt tcgggttccg gcttggctgg
     4561 ctttccaatt ttatcggcaa gccttctgtt catctcctcc ggaggcggga aaatcaattg
     4621 ctggccagca tagatcaagt caatatcctt gatctgggga ttctctttct taagaatttc
     4681 ggcgatctga gagtcgaaca tgccgtaggt tttaaaagag agatagctga gcgtatcccc
     4741 tttgcgcacc tgaaatttca gggaatcctc ggcgaaaata acgccggcca agggggaaag
     4801 gacaagcaga atcagaaaaa accgcaagta ccgctccgga gagaaaggtt tcataacggt
     4861 tctccacgaa acttagtagg tttttccttg aaatatcaca acctctttgg agatacaagg
     4921 aaaatccaag ggatgcgatg gttccgccca ggcctaagga ctcggattga aggaaggcaa
     4981 gggattattt cgaagatttt ttgggcccga aaagcttcgg ctcctcttcc tgagtttgcc
     5041 cggaggacgg tgcaggtttt gatttttcct ttttggcttt ggaataaaaa cgatccacaa
     5101 attccttggg aagcgaattc tgggtcttca caaggcccac cgggatgaga atttcattcc
     5161 caacgtaaat ccggttaggg tcgagattcg tgttagtctg ggcaatggtt ttccagtttc
     5221 cctggttgcc cgtgtaccag agggatataa tcgacagggt ttccccgctc cattttaccg
     5281 tgtgagcgat atgcgtctcc cggggtttag gctcggcctt gacctgggga ggcggcggag
     5341 cttcatgctg cgcaggggcg ggtgcaaccg atgaaagggc agccttctga acagaaggga
     5401 cgggttcccg ggtcttcaat ggaaccggtt ccttttcccc ggttggaggc ttggggttct
     5461 ggagggtagg agcgggctcg gtccgcggag gaggtccctc ctttttaaag ggtaaaaagg
     5521 cacagctcag gcagttgacc ccgaaggaga gtccaaaagc cca
//
LOCUS       DASPRR010000653         4335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_315769, whole genome shotgun sequence.
ACCESSION   DASPRR010000653 DASPRR010000000
VERSION     DASPRR010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315769"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..2495
                     /locus_tag="VEL68_23770"
     CDS             465..2495
                     /locus_tag="VEL68_23770"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXZ39068.1"
                     /translation="MEYPSFLRIFSRLPVANRLSLRTKLVFSFLFVVIAGGALSSLIG
                     TSLVADTIVSQARNKVRYDLSTASLVYSQSLSRINDVVQLTASGRTIQDYMESGRLKA
                     LADYLVKRRKDFGLDILTLTDPQGKVLLRTYHPYQSGDSQAGDPLVQKALKGETAAST
                     VIVSREDLMKEGSDLAERAFTLFVPTPKAREVPADRETSGMMLKAAAPIWKEPGTLAG
                     ILYGGVLLNRNYQIVDKVRDLLYGEGRYRGKEMGTATIFQGDLRISTNVRDENGGRAI
                     GTRVSEEVYRAVVQKGQVWLDRAFVVHDWYITGYEPIRDLSGRIVGILYVGMLEAPYV
                     DLRDKVVYSFFGIGVLGVLLVLLLSFFITTGIIRPLKEMVAATRKIAEGDLSHEVPIS
                     SKDEIGQLAESFNHMLVRLKQARQELEDYGRVLEEKVEERSQQLQTIQAKLMRSEKLS
                     SLGRLASGVAHEINGPLNGILTFSHLLMRRLKDNPELQKELEIITREATRVSSIVRGL
                     LDFARESKPQRRPCNINELILHTFSLVERQAVFQNIRIIRNLGSQLPILLLDANQIQQ
                     VFMNILLNAADAMPQGGTLTITSSYVPGDSFLQVKFTDTGCGIPEENLNQIFDPFFST
                     KADKKGTGLGLAVSYGIVDRHRGRIEVQSEVGRGATFTIHLPLEAAEEVPPA"
     gene            2932..3465
                     /locus_tag="VEL68_23775"
     CDS             2932..3465
                     /locus_tag="VEL68_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXZ39069.1"
                     /translation="MAKEPPAEGLDKLQEIFKEHEGNRGHLLIVLQKIQAIYGYVPPF
                     TFEMVAQALGATKSEIYGVLTFYNRFHLNPRGKHTIRACRGTACHFKGAPSIIETIKS
                     HLKLPPGKETTDDFLFSLEEVACLGACGISPVMAVDEETFGNLTPARAAEVLSQYQAQ
                     GSEKVAAGEAGEKQKAN"
     gene            3507..4058
                     /locus_tag="VEL68_23780"
     CDS             3507..4058
                     /locus_tag="VEL68_23780"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXZ39070.1"
                     /translation="MPHLKGSLHVHTHYSDGAHAPEEMVEAYRNLGYDFVAITDHDYL
                     IKPQYWESIPERNGDLIVFKGIELEYPPLRFQHLGKILGENENLLIFNHPNQYRLSAA
                     QVNQQIKVISRDMEIHCVEVTDKGVYTKMYDTPEIPLPKIASDDAHTFDMCGWAWVEV
                     QSPRDRDSILRAIKDGNFKIGLK"
BASE COUNT          982 a         1239 c         1184 g          930 t
ORIGIN      
        1 cttttaaaaa taatttttct ggcgcttctc ccaatcgagt ggctgggaag ggaacagaaa
       61 tattgtcagc ccggggaatg cgttccgccc atggcgggac agaatcggcg ggaaaaaccc
      121 ggattccgga gcgatagttt gggttccgca acggcacctg tccccgaccc cctttcgaaa
      181 aaccgaatct tggctcaggt ccagataacc aaaaatcttt ctgacccaaa ccgatgaaag
      241 gactcacgcc tcattctgtc ccgccatggg cggaacgtac ccctaggcct cgccgaatcc
      301 cccctggcct acagcgagca ttctggccga ttgaaaatcg accgaaatca cccactcgat
      361 tgggaatttt tcttttgggc tggatgcaag gtcctggctt tcattgactt ctgccaactg
      421 cctatctata atgtgggcca aaccggtcgc tttcttggca aagtatggag tacccctctt
      481 tcctccgcat tttctctcgg cttcccgtgg cgaatcgctt atctcttcgc accaagctgg
      541 tctttagttt tcttttcgtg gtcatcgccg gaggggctct ctcttctcta atcgggacca
      601 gtctggtggc cgacaccatc gtctctcagg cccggaataa ggtccgttat gaccttagta
      661 cggcctcgct ggtttacagc caatccttga gccggatcaa cgatgtggta cagctcaccg
      721 cttccgggcg gacgattcag gattatatgg aatcaggacg gttgaaggcc ttggcggact
      781 acctggtcaa aaggcgcaag gattttgggt tggatattct gaccctgacc gacccgcagg
      841 gaaaggttct gctgagaacc taccaccctt accagagcgg agactcccag gccggcgacc
      901 cattggttca gaaggccctg aagggggaga ccgcggcctc cacggtcatc gtctcccggg
      961 aggacctgat gaaagaggga agcgacttgg ccgagcgggc cttcaccctt tttgtcccca
     1021 cccccaaagc aagggaggtg cccgcggacc gggagacttc gggaatgatg ctcaaggccg
     1081 cggccccgat ctggaaagag cccggcacgc tggccggaat tctttacggc ggcgttctgc
     1141 tgaaccgcaa ctaccagatc gtggacaagg ttcgagacct tctctacggg gaggggcgct
     1201 acaggggcaa ggagatggga acggccacca tctttcaggg agacctccgt atttccacga
     1261 acgtgcggga cgagaatgga ggacgggcca tcggaacccg ggtctcggaa gaagtgtacc
     1321 gggctgttgt ccaaaaaggg caggtgtggc tcgatcgggc ctttgtggtc cacgactggt
     1381 acatcaccgg ttatgaaccc atccgggacc tgtccggccg gatcgtggga attctctacg
     1441 tgggaatgct ggaagccccc tacgtggacc tgcgggacaa ggtggtttac agcttctttg
     1501 gcatcggcgt gctgggcgtg ctcctggtgt tgcttctctc ctttttcatc accactggga
     1561 tcatccgccc tctcaaggaa atggtggcgg ccacgcgcaa gatcgccgaa ggggaccttt
     1621 cccatgaagt cccgatttcc tccaaggacg aaatcgggca gctggccgaa tccttcaacc
     1681 acatgctcgt tcgcctcaag caggcccgcc aggagctgga agattacggg cgggttctgg
     1741 aagaaaaggt ggaagagcga agccagcagc tccagacgat ccaggccaag ctgatgcggt
     1801 cggagaaact gtcttctctc ggccggctgg cctccggggt ggcgcacgag atcaacggcc
     1861 ctctcaacgg catcctgact ttctcccacc tcctcatgcg ccggttgaaa gacaaccccg
     1921 agctccagaa agaactggag atcatcactc gggaagcgac ccgggtctcc tccattgtcc
     1981 gggggcttct cgactttgcc cgggagagca aaccccagag gcggccctgt aatatcaatg
     2041 agctgattct ccataccttt tctttggtgg aacggcaggc ggtttttcaa aacatccgga
     2101 ttattcggaa tctgggctct cagcttccca tccttctcct ggatgccaac cagatccagc
     2161 aggtttttat gaacatcctg ttgaatgccg ctgacgcgat gccccaagga ggaaccctga
     2221 ccatcaccag cagctatgtc ccgggagact ctttccttca ggtcaaattc accgacaccg
     2281 gctgcggcat ccccgaggag aatttaaacc agatcttcga tccctttttc agcaccaagg
     2341 cggataagaa ggggacggga ttggggctgg cggtaagtta cggaatcgtc gaccggcacc
     2401 gaggccggat cgaagtgcaa agcgaagtgg gccggggtgc cacgtttact attcatctgc
     2461 ccctggaagc agcggaagag gtaccccccg cctaaagaac cgcctcgaat cgccgggggc
     2521 gcgttgctgg ttgctggttt tccgtcttta acaagcaacg agccctaaat gtagggtgcg
     2581 ttccccgaac gcaccgatac tttaggcctg ctcggggatc aggccctaga taatgtcacg
     2641 tctgcattag catagggcag ccagcaacga tttttcccta ttcccaaatg aaataaaaaa
     2701 tgttataaac gactttctgg aaacacagtt tcttcggact tcctcgcttg ctttccgcag
     2761 cagcccgagg tcgcccggga ttgcagggag ggggtccttt ccggaaactg cggggtctgt
     2821 gggacggtgg agtatctcgg gaccaaatcc cttcggtttc tggcctgttc gcgtagccct
     2881 cgggaatccg attcgaagga taaaaccaaa ggcctagagg aggaataggt aatggccaaa
     2941 gagccgcctg ctgaaggtct agacaagctg caggaaatct ttaaagagca tgaagggaat
     3001 cgaggacacc tgctgatcgt acttcaaaag attcaagcga tttacgggta tgttcctccg
     3061 ttcacctttg aaatggtcgc ccaagccctt ggggccacga agagcgaaat ctacggggtt
     3121 ttgaccttct acaaccgctt tcatctcaat ccccggggaa aacataccat acgggcttgc
     3181 cggggaacgg cctgtcattt caagggcgct ccctcgatca ttgaaaccat caagtcccat
     3241 ttgaaattgc cccccggcaa ggagaccacc gatgacttcc tcttcagcct ggaagaagtg
     3301 gcctgcctgg gcgcctgcgg gatctctccg gtaatggccg tcgacgagga gaccttcggg
     3361 aatctcactc cggcccgggc cgccgaggtc ctttctcagt atcaagccca gggctccgag
     3421 aaggtcgcag ccggggaagc cggcgaaaag caaaaagcca actgaaagcc ccgggtagag
     3481 tctgacggtg aagagagaat ctcggcatgc ctcacctgaa agggagtctc cacgttcaca
     3541 cccattattc cgatggcgct cacgccccag aggagatggt tgaagcctac cggaacttag
     3601 ggtatgactt cgtggccatc accgaccacg actacctgat caaaccgcaa tattgggaga
     3661 gcattccaga aagaaacggt gacctgatcg tcttcaaagg aattgaattg gagtacccgc
     3721 cccttcgttt ccagcacctc ggcaagattc tgggcgagaa tgagaatctc ctgatcttca
     3781 accaccccaa ccagtaccgt ctttccgcgg cccaggtcaa ccagcagatc aaggtgatct
     3841 cccgggacat ggaaattcac tgcgtggagg tcaccgacaa gggtgtttat accaagatgt
     3901 acgacacgcc cgaaatcccc ctgcccaaaa tcgcctccga cgacgcccac accttcgaca
     3961 tgtgcggttg ggcctgggta gaggtccaga gcccacgcga tcgggattcc atcctccggg
     4021 caatcaagga cggaaacttc aagatcggat taaaataatt gggcattaac cagtgatcaa
     4081 tgatcattgg ccattaggcc tattccgttg actccaccaa gccctatata tggtggtgtt
     4141 agctcatcag gaactaccac tacttgtatg aacttgagtc aagtgaatag ccctattggc
     4201 cattgatcat tgggcattga ccagtgatcc ccgggctccc tttattcaaa aaaactcacg
     4261 tcccccttcc ctttgcgcac aatcttgggg gtgtccccgg tcaggtcgat gacgctggag
     4321 ggttcggaga ccagc
//
LOCUS       DASPRR010000654         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_320381, whole genome shotgun sequence.
ACCESSION   DASPRR010000654 DASPRR010000000
VERSION     DASPRR010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320381"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1276)
                     /locus_tag="VEL68_23785"
     CDS             complement(293..1276)
                     /locus_tag="VEL68_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HXZ39071.1"
                     /translation="MKIPEFKDVLLAQCRIRPYLPRTPLHSYPAVNQLVGTEVYIKHE
                     NYQPVGAFKVRGGINLISQLSSEERKRGVISASTGNHGQSVAYAAGKFGVQARIVVPE
                     KANPGKVAAIQGMGAKVIFHGVVFDEARLHCEALAQEKGYRYIHSGDEPLLIAGVATE
                     VLEMLEDQPDLQVIFIPVGGGSGAAGACIVAQAVNREIRVVGVQSEASPAAYQSWKQK
                     KMVEAPNRTIAEGLATGTAFYLPQAILWEYLKEFILLPDAEILRAMTWMVERAHTLAE
                     AAGAAPLAAAYHLREELKGKKVGLICSGGNVSLEQLKQALLNAEFGMRSAE"
     gene            complement(1273..2202)
                     /locus_tag="VEL68_23790"
     CDS             complement(1273..2202)
                     /locus_tag="VEL68_23790"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXZ39072.1"
                     /translation="MIKKNKYISSGLFFIFFSGALIFIAKGAFGSPISLTWVHATVRV
                     ENEWGKGGTGFLVIRKMEKKQGKVFLVTNKHVIHPDPESREKAHFLTLFLNVREKDGT
                     VAGKSLQAPLIEDDQKLWREHPNPHVDVIAVDITSLINSYPNLENRGADYSLFATPQI
                     LKEETITEGEEVLILGYPLGLFHTRIHSPLVRQGIVATKIGERIQVRLRSSSGDPLRV
                     EIPGFLIDAAIIPGSSGSPVVLKPIIGRKIKDKVEMGTAVPYLLGIVSATETTAIRME
                     GYAFPTLAGLGIVFDASTIQETIELFFPKERRP"
     gene            complement(2199..2771)
                     /locus_tag="VEL68_23795"
     CDS             complement(2199..2771)
                     /locus_tag="VEL68_23795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXZ39073.1"
                     /translation="MDNLYARIEGPFADSDRIRPCAVGDFEEIYEIINDAAQAYQGVI
                     PADRWKVPYMPREELKDEIEAGVVFWGYKEKDQLLGVMGLQEVQDVTLIRHSYVRTAR
                     RNAGIGGKLLSRLKAQTGRPLLVGTWAVAVWAIRFYEKHGFRLVSPEDKEKLLKKYWS
                     IPDRQVETSVVLADPKWFLVFERGGTGFQR"
BASE COUNT          847 a          978 c          960 g          923 t
ORIGIN      
        1 atgataatgc agggtttcat gggtcttcgc aagaatggcc aattcctaat actccatggc
       61 ctaggtccga gaatcgaatg ttcgggcctc gaaaattgat gaactcgtaa taagtcgaaa
      121 ttccataaaa tttgtcattc cggcgaaagc cggaacccag ctattcaaag atattctgga
      181 ccccggcttt cgcgggggtg acaatccaag agacttttta cgagaccatc aaaattgatc
      241 aatgaaaatc atttggcatt tggacctgac cagtacccag taacccttat ccctattccg
      301 cactccgcat tccgaattcc gcatttaaaa gggcttgttt caattgttcc agggagacat
      361 ttcctcctga acagatgagg ccgactttct ttcccttcag ttcttcccgc agatggtagg
      421 cggcggccag gggcgcagct cccgcagcct ccgcgagggt gtgggcccgc tccaccatcc
      481 aggtcatggc ccggaggatc tcagcatcgg gaaggaggat gaattctttc agatactccc
      541 acagaatcgc ctggggaaga taaaaggcgg tcccggtggc cagcccctcg gcgatggtcc
      601 ggttgggggc ttccaccatt ttcttctgct tccaagattg gtaggcggcc ggagaggcct
      661 ccgattggac cccgacgacc cgaatctcgc gattcaccgc ctgggccacg atacaggctc
      721 cggccgctcc gctcccccct cctacaggga taaagatcac ctgcagatca ggctggtcct
      781 ccagcatctc cagaacttcg gtggccacgc cggcgatgag caggggttcg tctcctgaat
      841 gaatgtaacg gtatcccttt tcttgggcca gggcttcgca gtgaaggcgg gcttcgtcaa
      901 agacgacccc atgaaaaatg accttcgctc ccattccctg aatggccgcc actttacccg
      961 gattggcctt ttccggcaca acgatccggg cttgaacgcc gaacttcccg gccgcgtagg
     1021 ccaccgactg gccgtggttt ccggtggagg cggaaataac cccccgtttt cgttcttccg
     1081 agctcagctg ggagatcaga ttaatccctc cccggacctt gaaagccccc acgggctggt
     1141 aattttcatg tttgatatag acttcggtac ccaccagctg gttgacggcc ggataactgt
     1201 ggagaggagt tcgggggagg taagggcgga tccggcattg ggccagcaga acatccttga
     1261 actcagggat cttcatgggc ggcgctcctt ggggaaaaac aactcgatag tctcctggat
     1321 cgtcgaggca tcaaaaacga tccccagtcc ggccagggtg ggaaaagcat atccctccat
     1381 gcgaatggcc gtagtctccg tggccgagac gatacccagg agatagggga ctgctgttcc
     1441 catttcgact ttgtccttta ttttcctccc gatgatgggt ttgaggacca ccgggctgcc
     1501 gctggagccg ggtatgatcg cggcatcgat gaggaatccg gggatttcca cccgcagggg
     1561 gtccccggag gaggagcgaa gtcgaacctg aattcgctct ccgatttttg tggccacaat
     1621 cccctggcgg accaaagggg aatggatgcg ggtgtggaag aggcccaggg gataaccgag
     1681 gatcagcact tcctcacctt cggtgatggt ttcctctttg aggatttgag gagtggcaaa
     1741 aagggagtaa tccgcgccac ggttttccag gttgggataa ctattgataa gagaggtgat
     1801 atccacggct ataacatcca catgaggatt cgggtgttcc cgccagagct tttggtcatc
     1861 ctcgatgagg ggggcctgta aggatttccc tgccaccgtt ccgtccttct cgcggacatt
     1921 cagaaaaagg gttagaaaat gggccttctc ccgggattcc gggtccggat ggatcacgtg
     1981 cttgttggtg actaagaaca ccttgccctg tttcttttcc atcttgcgga ttaccagaaa
     2041 ccccgttccg ccctttcccc attcgttttc cacccggacg gttgcgtgaa cccaggtgag
     2101 ggagatggga gagccgaagg ctcctttggc gatgaatatc aaggcccccg agaaaaaaat
     2161 gaagaaaaga cctgaagaga tgtatttatt cttcttgatc atctttgaaa tcccgtccct
     2221 cccctctcga ataccagaaa ccatttggga tcggccaaaa cgacggaggt ttccacctgg
     2281 cggtccggaa tggaccaata ttttttgagc agcttctctt tatcctcggg agaaaccaga
     2341 cggaacccat gcttttcata aaaccgaatc gcccaaacag caacggccca ggtcccaacc
     2401 agaagagggc gccctgtttg agctttcaag cgagataaca gtttcccccc aatccccgcg
     2461 tttctccggg cggtccggac ataggagtgg cggatgaggg ttacatcctg aacctcttgg
     2521 agtcccatca cgcccaggag ttggtccttc tccttataac cccagaaaac cacacccgcc
     2581 tcgatctcgt cctttagttc ctcccggggc atgtaaggga ccttccaccg gtccgcagga
     2641 atcactcctt ggtacgcctg ggcagcatcg ttgatgattt cgtagatttc ttcgaaatca
     2701 ccgactgcgc aggggcggat tcgatcgcta tctgcaaagg gaccctcgat ccgggcgtag
     2761 agattatcca tggggcgcat aatccgaaat caggaatgat cggttataaa gctctacgga
     2821 tgttggggaa atgttaattg catttaaaat agcacaatct ggccattcag aaaagggaaa
     2881 ggaagggagc cttataagtt atccgggcga atccatagcc taaattgtac acttctgtga
     2941 cctttcatcc atggccggga cctctgagga tgctgtggtc tcttatactc ctgtatatct
     3001 gattagaatc atggggtttt atcatcaatc cctttgagga actttcctcg tcgtatggca
     3061 tccatcctgc aatttggcat gattgcaatg tcaataaaat caaacgccct tttgggaaaa
     3121 acggaaagaa ggtcttttag agaccgacgg agccaaccca cccccctcct tagccgatac
     3181 accttatggg gccgaagaaa gaccctgcgc agaaaagagg atcaagaaag gggcgggtat
     3241 gtggaccgtt atgattctaa actgctcttt tttatagtct tgattgttgg gcttaatgtc
     3301 ctcgattccc tattcaccat gatcattttg gactgcgggg gttgggagat aaacccgatc
     3361 gtttgcttgg caatcgaggt ttatggagat cagttttggg tctggaagtt catgattgtt
     3421 tcagtgaatt tggttctctt atgtctccac agcaggtttc ggtatgtaaa tagaatcatt
     3481 tcgtatatta cgatcctgta cctgggaatt atcttctacc aggttttcat tatgaatttt
     3541 cacatcttta aatgaaaaag gggctgtcac gagaaacacc ggatccccca ttgtcttgtc
     3601 cgctggagtt cctcatcctg cctactttgt aatccccatc tccttcatga tcttggcgta
     3661 catgttgctt tgggcctccc aaatctttta agtctcggtg ctggtggt
//
LOCUS       DASPRR010000655        14286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermodesulfobacteriota bacterium isolate SMAG_U12230
            k141_320910, whole genome shotgun sequence.
ACCESSION   DASPRR010000655 DASPRR010000000
VERSION     DASPRR010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945146
            Sequence Read Archive: SRR7004335
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermodesulfobacteriota bacterium (soil metagenome)
  ORGANISM  Thermodesulfobacteriota bacterium
            Bacteria; Thermodesulfobacteriota.
REFERENCE   1  (bases 1 to 14286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:30:21
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,737
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 18 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14286
                     /organism="Thermodesulfobacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320910"
                     /isolate="SMAG_U12230"
                     /isolation_source="soil"
                     /db_xref="taxon:2202153"
                     /environmental_sample
                     /country="Colombia"
                     /lat_lon="4.2396 N 73.2024 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VEL68_23800"
     CDS             <1..332
                     /locus_tag="VEL68_23800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39074.1"
                     /translation="HNPFVVSLSNHERITTQSLEGEGGREGEMKKRIVILMEEEVVKQ
                     AQERASQEGRTLGDLIQDALVSYWGNKEQVQRTREGAYQLFCERPIRVSRKQFNELLK
                     EESFKPL"
     gene            1172..1828
                     /locus_tag="VEL68_23805"
     CDS             1172..1828
                     /locus_tag="VEL68_23805"
                     /inference="COORDINATES: protein motif:HMM:NF040080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HXZ39075.1"
                     /translation="MSHRGLSSNKGYQWRGNEGEICSGRRYFRVSSLVVIVAKMPPTI
                     IRPSGAIVLRSKRKVNDAMKEKKIVEEKAQQLIGKTGSFCKKFLDEDYKQLCEKLILK
                     MSRKRTVPFLSGRMEIWAAAVVYALGSINFLFDKSFKPHATAEDICNYFGTSKSTTSQ
                     KSKVIRDMFRMGYYDSEFSTKHMKNNSPFSNMVMINGLIVDKRHLAPEIQEMLKREGE
                     "
     gene            1917..2567
                     /locus_tag="VEL68_23810"
     CDS             1917..2567
                     /locus_tag="VEL68_23810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39076.1"
                     /translation="MARKTPLNGICEICKAPVSKGGVKKHLQAHFEEGGKKENAAGGQ
                     RRSGKLFHLTVEGYGLSGVLYWMHLKALGSARLRDLDTLLRDTWLECCGHMSVFSNRE
                     EDFEMGVKLKDILKPGLKLNYEYDFGSTTQLLLTVVSEIEGSIKKGQVEILARNEAPR
                     IKCSHCEKPATTVCTECIYDNQGWLCGDCARKHDCDEEMFLPLVNSPRAGVCGYTG"
     gene            2567..4042
                     /locus_tag="VEL68_23815"
     CDS             2567..4042
                     /locus_tag="VEL68_23815"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXZ39077.1"
                     /translation="MGIKSLKNSKVLEVIRRLSRKRKEEVYLVGGAIRDFLLGRPLGR
                     DFDFVIKGDVEGLAKEISRELGGNAFPLDETFGTWRVILKKKKKKTELDFSPLQGKDI
                     FEDLRQRDFTVNSLAIRLKDLARPGEPCFIDPLDGLVDVRKRILRANSEESLHQDPLR
                     MLRAFRFASTLKFSVEEETLKMIHKNSDLIRQSAGERIRVEFFMALGEEGAGLFLRSL
                     HQAGLLGEIFPEVREWETLNVGTASSPSLLDHALRTLEAAEFIFVHMKDIFSEWADVL
                     KGHLSHSEEEGVSRIALLKFIAFFHDSGKVATACQEKGSISPRFLDHDQEGQEINMAI
                     ARRLKLSRRSVRIISELTRQHMRIQSLAKAQELTGRAKYRFFQDLGKEGLDLAILSLA
                     NALASSGLELRSPMLAEIRKKAKKIKKITDDLVRFYTEEFAQKSAKPLVDGKEIMGTL
                     GIPQGKAVGTLLNKLKEEEISGRVRTREDALQFLKNIDKSG"
     gene            4071..4145
                     /locus_tag="VEL68_23820"
     tRNA            4071..4145
                     /locus_tag="VEL68_23820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4103..4105,aa:Gly,seq:ccc)
     gene            4280..4741
                     /gene="rimP"
                     /locus_tag="VEL68_23825"
     CDS             4280..4741
                     /gene="rimP"
                     /locus_tag="VEL68_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXZ39078.1"
                     /translation="MKSETLLQEVREVVEPILQSQGYELVDLEYQRESRGWVLRIYLD
                     REGGITLDDCTGVSHEVGAVLEVKDVIPNAYVLEVSSPGLTRPLKKPEDYNRFRNQLV
                     KIKLFQPLDGRRNFKGILLGLEGETVRVEVDQQLFEIPLQSIAKANLEIDF"
     gene            4758..6032
                     /gene="nusA"
                     /locus_tag="VEL68_23830"
     CDS             4758..6032
                     /gene="nusA"
                     /locus_tag="VEL68_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXZ39079.1"
                     /translation="MASDLSRIIDQVGKDKGIDRKILIEALEQALLAAARKKYGTKQE
                     IEAHYSEESGEVELFQFKTVVKEVKNPPAEISLKEAKDLDSEVQVGDSLGTKIDVSEF
                     GRIAAQTAKQVIIQRVKDAERENIFDDFKDRKGEVISGTVQRFERGSIIVNLGRAEAV
                     VPQQELIPKETYKPGDRIRAYVLDVRKTPKGPQIILSRSNPNFLIKLFEMEVPEISEG
                     IVKIINAAREPGSRAKIAVYSRDSDVDPVGACVGMRGSRVQAVVQELRGEKIDIIPWS
                     EDAASFVCNALAPAEISKVIINPEAKSMEVIVEDDQLSLAIGRRGQNVRLAAKLTDWK
                     IDIRSESKAERPVPTLDEIGTRGMDTVEKVTGVGEKTAQLLNQAGFLTVADLSQANLD
                     QLTKIEGIGPKKAEKLLEAARAHLDQSEEKKA"
     gene            6091..8913
                     /gene="infB"
                     /locus_tag="VEL68_23835"
     CDS             6091..8913
                     /gene="infB"
                     /locus_tag="VEL68_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXZ39080.1"
                     /translation="MVKVRIHELAKDLKMENKDLIKLLEKMGLPVKSVQSSLDESDVE
                     RVKNQISLSKKEGIVEQRVKPTVIRRRVMKEEPTPEPPPPPPKEEVKPAVEAKPPVAK
                     KGEVRPPAVKKEGKPEAAKVLAEAPVEVPKKKVEIKAEAPSPKAEGVEPGPEPTAIPV
                     PPVVPPPPVKEEEKKVEAPVGIKPAPGAEIPLKPKAAEPRGEGVAPAEKPKPPEKPPK
                     PKKKYEPAKIIERPPVPPPSLVKEREHVPMEKRPAAPPRPPIILQRPTAPGPGRPAPP
                     AAAAEEERGRRKKKLKKDVEVLEEGKPDRVRVISTKKRAFKEYDYQREEQGARPFVFE
                     REPKQRVVARTKMAKPEITVPKAIKRKIRISEAILVGELAKRMGVKVSEVIKKLMGLG
                     LMVTINQAIDADAASLVAADFGYEVEKTALELEDLIEEREDKAEDLQPRPPVVTIMGH
                     VDHGKTSLLDSIRQTNVTAGEAGGITQHIGAYTVDVDHRKVVFLDTPGHEAFTAMRAR
                     GAKVTDIVILVVAADDGVMNQTVEAINHAKAANVPLIVAINKIDKPNANPDRVKQNMT
                     EYGLVPEAWGGQTIMAEISAKQKVGLQELLELILLQAEVLELRANPNRAMTGTVIEAK
                     LDRGRGPVATILVQDGTLHVGDSFVCGSYFGRVRAMINDRGEKMEEAGPSTPVEVIGF
                     SSVPQAGESFVVVTDEKKAKDLAAQRTQKQRELELAKSSRVTLEDFYKKMKKGVVKDL
                     NLILKADVQGSIEALTEALGRLSTEAVRVNILHSSVGAINESDCMLASASEAIILGFG
                     VKMEPSAQGVAEQEKIDIRFYNVIYDAIEDVRKAMEGLLEPIYEKRLTGKAKVIQLFT
                     ISRVGVVAGSMVFEGKILRGSKAHVVRDGKTIHEGEIATLKRVKDDVKEVVSGFDCGI
                     TVGGFQDFQLNDVIESYVVEKVTAHL"
     gene            8931..9218
                     /locus_tag="VEL68_23840"
     CDS             8931..9218
                     /locus_tag="VEL68_23840"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HXZ39081.1"
                     /translation="MVIGLCHLELLLDGNFSLKGKRQVIKSIVDRARQRFNISIAEVE
                     DQDLWQKAVLGVCTVANDRQRVNSILDKVVSFIENTNLAQVTDSQIEIMNV"
     gene            9369..9740
                     /gene="rbfA"
                     /locus_tag="VEL68_23845"
     CDS             9369..9740
                     /gene="rbfA"
                     /locus_tag="VEL68_23845"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXZ39082.1"
                     /translation="MVYKRSDRVGDMIRQVIGEMLRRDLNDPRLSLVTLTEVKVTEDL
                     KIATVFFSAMGNPVQEEASLHGLQSAAGYIKKRLGRELRLRFVPELLFKVDHSFDYGK
                     RIDRLIESVQDEEKGDPPEDR"
     gene            9697..10674
                     /locus_tag="VEL68_23850"
     CDS             9697..10674
                     /locus_tag="VEL68_23850"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HXZ39083.1"
                     /translation="MSKTKKKEILRRIAEEIRGRRSFLISTHQNPEGDAIGSVLALGL
                     ALKSLGKEALILTQDPVPEVLAFLPGSGEIVHQAPVNRGFDIALAVDCGDRPRLGEEF
                     SKVQGIGKIINIDHHVSNGFFGDLNLIDPKASSAAEIIYDLIQALPAPFTREVAENIY
                     TGLLTDTGSFHYSNTSPKTFSVARACMLAGVDPWEVSQKIYDTQPLPRLRLLPRVLDT
                     LELLENGRVSSMVVTQQMLKETVATVAMTEDFINFARSVQGVEVALLFREVTSRKYRV
                     SMRSRGRVDVARIAGQFQGGGHPAASGCTVEGSLSEVRTQVLEVVKAAF"
     gene            10671..11600
                     /gene="truB"
                     /locus_tag="VEL68_23855"
     CDS             10671..11600
                     /gene="truB"
                     /locus_tag="VEL68_23855"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXZ39084.1"
                     /translation="MNGVLVIDKPKGWTSHKVVAWVRNALRLRKVGHGGTLDPLATGV
                     LPIYLGEGTKLVPFNLEGVKEYIATMKLGQETDTLDADGRIVAEKEGFLCTRQTVEEV
                     LHQFRGKIRQRPPLYSAVKQQGVPLYKRARSGETPEVAERDAVVHALFIKGFSFPLVT
                     LEIVCGRGTYIRSLCADMGRVLGCGAHLVELRRTRSGRFGLDQAMSLEDLSRWIEEKR
                     IEEKVIPLKNGVDLSGEVRLGEKAAARVRQGQSLCLNDLPEGERKRLQKGQRWGLFQE
                     AQGLVAIAESQVDAESQLSGDSQALRILRVFQT"
     gene            11697..11966
                     /gene="rpsO"
                     /locus_tag="VEL68_23860"
     CDS             11697..11966
                     /gene="rpsO"
                     /locus_tag="VEL68_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXZ39085.1"
                     /translation="MTLLPERKKAIIEGNKRHPTDTGSPEVQVAILSERINYLTEHFK
                     AHTKDHHSRRGLLQLVGQRRHLLDYLKKKDVERYRSLIQKLGIRK"
     gene            12075..>14286
                     /locus_tag="VEL68_23865"
     CDS             12075..>14286
                     /locus_tag="VEL68_23865"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXZ39086.1"
                     /translation="MSTLGNRLEVRCNLGGRDFIFETGAVAKQADGAVLVTFGESMVL
                     VTATASRTIREGIDFFPLVVDYQEMAYAAGKIPGGFFKREGRPSEKEILTARLIDRPL
                     RPLFPKGFFNEVQIVATVLSADQENDPDVLAICGASTALQISDIPFQGPIAGVRVGRI
                     QGQWVCNPTGSQLAQSDVNLVVAGSREGVVMVEGGGQEISEEELLEAIFFGHRSLQPI
                     LDIQEELKKKKIEQENEQRRAAGEAEIADIVKRRAPQLFPDPVLGKSVLLADESIRER
                     VRTAGQEKILQALSISKKKVRTEQLEQAREETLKALLEEFPERAGEIKAYYEAVEKFV
                     VRRPILQENRRVDGRRFNEVRPIHIQVGWLPRTHGSARFTRGETQVSAVVTLGSAEDE
                     QKIDALTGETFKSFMLHYNFPPYSVGEVRMLRGPSRRDIGHGALAERSINRILPPSDK
                     FPYTVRIVSEVLESNGSSSMATVCGSSLALMDAGVQIKRPVAGIAMGLIKEGEKVVVL
                     TDILGDEDHHGDMDFKVAGSRSGITGFQMDIKISGVTREILRQALAQAREGREFILQK
                     MEEALAGPRVEMSPYAPRFLSLRIKPDRIREIIGPQGKVIRGIQDETGVKINVEDDGS
                     VHIFSPDSQAAQKAANIIRDMIKEAEVGQFYMGRVTSIARKQDGKEFGAFVEIFPGTD
                     GLVHISQLAKERVRNVEDVLKEGDQVLVKVIGIDERGKIKLSRKEALGHPWPENK"
BASE COUNT         3605 a         3564 c         4273 g         2844 t
ORIGIN      
        1 ctcataatcc gttcgtggtg agcttgtcga accatgaacg gattacgaca cagtctcttg
       61 agggagaggg cgggagagag ggggaaatga aaaaaaggat tgtcatcctc atggaggaag
      121 aagtagtcaa gcaggctcaa gagcgtgctt cccaagaagg aagaaccctg ggcgatttga
      181 tccaggatgc cctcgtatcg tattggggta acaaggagca agtccaaaga acgcgggaag
      241 gagcctatca gctcttttgt gagcggccga tacgcgtcag cagaaagcag ttcaatgagc
      301 tcctaaaaga ggagagtttt aagccgcttt aaaaccaaag actctcctct ttcttgccct
      361 tttcaatatt gttgaatgca acccccttgc aatttcctgc caagggtggc atgatcggta
      421 gccagaaatc cccttctcat aataaaatat cattcctggg tccaattgga ggttttcaga
      481 gggcagtaaa tatttcttac cgcaacggga aatatgagta atgacattgc cttgccgatc
      541 ggccaagagg tgttcttccc tgggcttttc gatgtccgcg tgatgctgga ggaggcctcg
      601 atgctgcaaa gctggtatga atgccgttcc tgcctccctg acggctgccc ggtaaaggca
      661 tgtttcccct ttagggaggc agcttcgctc gccaaatcct tccaaccatc aagaatcaac
      721 gcaaaatctg caaatcctag ggaaatttgc cttctaattg aagacgggca catatcaggt
      781 tggcttatac ctatgacagc cggtctaccg ttagcatttc ggggggtcaa tgggctccta
      841 tcaaagtgag gctttttagg gatcggggaa tgtagatggc cttaattctt tcggacaaag
      901 aaatagcgga tttggtccaa gaaaagaaat tcttgccgac ggattatcaa accaagattc
      961 agttgcggcc caaacccgga cacaaagagc gtgagctgga tcttaaaggc gaacaaggaa
     1021 gcgattttcg aagaccagga ggaaataaat cgaaaggtcc tcgcccgata tagtgatgtt
     1081 tgtgaaaagc aattttcaag gttagccgca aatcgtgagc gaataaaaaa gtttttagaa
     1141 gaatcccttc aatgaaggac accaaggatc tttgtcccat aggggactat cttccaataa
     1201 aggctatcag tggcgaggca acgagggaga aatctgttct ggaaggagat acttcagggt
     1261 ttcatctcta gtcgtaatag tggccaaaat gccccctacg atcatacgcc cctctggggc
     1321 aattgttctg cgttcaaaaa gaaaggttaa cgatgccatg aaagagaaaa aaatcgttga
     1381 ggaaaaagcg cagcaattga tcgggaagac ggggagcttt tgcaaaaagt tcctggatga
     1441 agactataag caattgtgcg aaaaacttat cctcaagatg tcccgaaagc ggacggttcc
     1501 ctttctttcc ggccggatgg aaatctgggc ggcggcggtc gtttatgcct taggcagcat
     1561 taatttcttg tttgataaga gttttaagcc tcacgctacc gctgaggaca tctgtaatta
     1621 tttcggcaca tccaaaagca cgacctccca gaaatcaaag gtgatccgag atatgttcag
     1681 gatgggatat tatgattcag agttctcgac aaagcacatg aagaataaca gcccgttttc
     1741 caatatggtc atgatcaatg gtttgatagt ggacaaaaga catctcgctc cggaaattca
     1801 ggaaatgctc aaacgggagg gggaatagaa caagcctgtt caacgagagt gacaagagaa
     1861 agaattgtgc ttgaggttta gagaattttt tagaaatttt aatagggagg ccggccatgg
     1921 cgagaaaaac gcccctgaac gggatctgtg aaatttgtaa agccccggta tcaaaaggag
     1981 gagtaaaaaa gcacctccag gcccatttcg aggagggcgg gaaaaaggaa aatgctgctg
     2041 gaggacagag gcgttcgggc aaactattcc atctcacggt cgaaggatac ggcctgtcgg
     2101 gtgtccttta ctggatgcac ctgaaggcct taggcagcgc cagacttcgg gatttggaca
     2161 cgcttctgag ggacacctgg ctggaatgtt gcgggcatat gagcgtcttt tccaataggg
     2221 aagaggattt tgaaatgggc gtaaaattga aagacatcct taagcccggg ctgaaattga
     2281 attatgaata tgatttcggt agtaccacgc agctgcttct aaccgtggtt tccgaaattg
     2341 agggcagcat aaaaaaaggc caagttgaaa tcctggccag gaatgaagct ccccggataa
     2401 agtgcagtca ttgcgagaag ccggccacga ccgtctgcac ggagtgcata tacgataacc
     2461 agggctggct ctgcggcgat tgcgcccgga agcatgattg cgatgaggag atgttcctcc
     2521 cattagtaaa ttccccccga gccggggttt gtgggtacac aggatagtgg gaataaagtc
     2581 gctgaaaaat tccaaggtcc tagaggttat tcgccgccta tcccgaaaga gaaaagagga
     2641 ggtctatctc gtcggcgggg cgattcggga ctttcttctg ggaaggccgc tgggtaggga
     2701 ttttgatttt gtgatcaagg gggatgtgga aggcctggcc aaggaaatat cgcgggagct
     2761 cggggggaat gcttttccgt tagacgagac ctttggaact tggagggtga tcctcaaaaa
     2821 gaaaaaaaag aaaacggagc tcgatttctc ccccctacag gggaaggata tctttgaaga
     2881 cctgaggcaa agggatttca ccgtcaactc cctggcgatc cgtctgaaag acttagcccg
     2941 gcccggcgag ccatgcttca tcgatccctt ggacgggctg gtggatgttc gcaaaagaat
     3001 cctgcgagcc aactcggaag aatccctcca ccaggacccc ttgcggatgc tccgggcatt
     3061 ccgtttcgcc tctaccctga aattttccgt agaagaagag acgctgaaga tgatccacaa
     3121 aaatagcgat ctcatccgcc agtcggctgg ggaaagaatc cgggtggagt tcttcatggc
     3181 cctgggagaa gagggcgccg gcttatttct ccggagcctt catcaggccg gcctcctggg
     3241 agaaatcttt ccggaggtca gggaatggga aaccctgaat gtggggacag cttcatctcc
     3301 atccctgctg gaccatgccc tgcgcacttt ggaggccgcg gaattcatct ttgtccatat
     3361 gaaggatatt ttttccgaat gggccgatgt cttgaaaggg catttgtctc attcggagga
     3421 agaaggcgtc tcccgaatag cccttttaaa gttcatcgcc tttttccacg attcgggaaa
     3481 ggtcgccacg gcctgccagg agaaagggag tatatccccc cgcttcctgg atcacgacca
     3541 ggaaggtcag gaaatcaata tggccatagc ccggaggttg aaactcagcc ggaggtctgt
     3601 gaggattatc tccgagctga ctcgccagca tatgcgcatc cagagcctag ccaaggccca
     3661 ggaattaacc ggccgggcta agtaccgctt tttccaggac ctgggaaagg aaggacttga
     3721 cttggccatc ctttcattgg ccaacgctct ggcttcaagc ggcctcgaat taagatcgcc
     3781 catgcttgcg gaaataagaa aaaaagcaaa gaaaatcaag aagataaccg atgaccttgt
     3841 acgcttctat acagaagagt ttgctcagaa atcggccaag cccctggtcg atggaaaaga
     3901 aatcatggga accctgggaa tccctcaggg aaaggccgta gggaccctgc tgaacaagct
     3961 caaggaggag gagatatcgg ggcgggttcg tacaagggag gatgccctgc aattcctaaa
     4021 aaatattgac aaatctgggt gatttggata ttatattttt gggtctttag gcgggcgtaa
     4081 ttcaggggta gaatgcaagc ttcccaagct tgacgtcgcc agttcgaatc tggtcgcccg
     4141 ctccactaag ccaatcataa attcttttct gtcaaggaat atggtctaca gctaactgaa
     4201 atgggttgtt tttttatggt ctacttgaaa aaagtgggct ctcgggccca cttttttttg
     4261 gtcgaaggaa cagattcggt tgaaatctga aacgctgctg caagaagtgc gcgaggtcgt
     4321 cgaacccatc ctgcaatccc agggatatga gttagtcgat ctggaatatc aacgcgagag
     4381 tcggggctgg gtcttgcgga tttacctgga tcgcgaaggg ggaatcacac tggacgactg
     4441 taccggagtg agtcacgaag tgggagcggt gctggaagtg aaagacgtga tccccaacgc
     4501 ctacgttctc gaggtctcct cccctggact cacccgcccg ctcaagaagc cggaagacta
     4561 taatcgattt cggaatcagt tggtcaagat caaactgttt caacccctcg acggccgcag
     4621 gaacttcaag gggatccttc tggggctgga gggagagacg gtacgagtag aggtcgatca
     4681 gcagctcttc gagattcctc tccagagtat cgccaaggcc aatttagaga tagatttttg
     4741 aggaagggga tgtagccatg gcttcggatc taagtcggat tattgaccag gttgggaaag
     4801 acaagggaat tgatcgaaag attctcattg aggctctgga gcaagccctc ctggccgccg
     4861 cccggaaaaa atacggcacc aagcaggaga ttgaggctca ctatagcgag gagtccgggg
     4921 aggtcgagct cttccagttc aagaccgtgg tcaaggaggt taaaaaccct cctgccgaga
     4981 tctctttgaa agaggccaag gacctggatt cggaggtcca ggtcggcgat agtttaggaa
     5041 ccaagataga tgtttcggag tttggaagga tcgctgccca gacagccaag caggtgatca
     5101 tccagcgggt gaaggatgcc gagcgggaga acatcttcga tgatttcaaa gaccgaaagg
     5161 gagaggtgat ttctggaacg gtgcagcggt ttgaaagggg gtccatcatc gttaacctgg
     5221 ggagagcgga agcggtcgtc ccccagcagg agttgatccc caaggagacc tacaagccgg
     5281 gagaccggat ccgggcctat gtcctggatg tccgcaagac ccccaagggc ccccagatta
     5341 ttctctcccg cagcaacccc aatttcctga tcaagctctt cgagatggaa gttccggaga
     5401 tttcagaagg catcgtcaag atcatcaacg ccgcgcggga acccggcagc agggccaaga
     5461 tcgccgtcta ttcgcgggac tcggatgtcg atccggtggg ggcatgcgtg ggaatgaggg
     5521 gctcccgggt ccaggcggtg gttcaggagc ttcggggaga aaagatcgac atcatcccct
     5581 ggagcgagga cgcagccagc tttgtttgca atgccctggc cccggcggaa atttccaaag
     5641 tcatcatcaa tccagaagcg aagtccatgg aagtgatcgt ggaagatgac caactctcct
     5701 tggccattgg gcgacggggt caaaatgtaa ggctggcggc caagctaacc gactggaaga
     5761 tcgacatccg cagcgaatca aaagccgaaa ggcccgtacc cactcttgat gagattggca
     5821 cacggggaat ggacaccgtg gaaaaggtta ccggcgtggg ggaaaaaacg gctcagctgc
     5881 tgaaccaggc tggcttcctc accgtagccg atctcagcca ggccaactta gatcaattga
     5941 ccaagatcga agggatcgga ccgaaaaagg cggagaagct gctcgaagcg gctcgagcgc
     6001 acctggatca atccgaagag aagaaggcat agaagagtct ttacccgaag cggtgggaag
     6061 tcttgaagcc gagtgaaggg ggtattggtg gtggtaaaag tcagaattca cgaactggct
     6121 aaagatttga agatggagaa caaagacctg atcaagctcc tggagaagat gggtcttccg
     6181 gtcaaaagcg tccagagcag cctggacgaa tcggatgtgg agcgagtcaa gaatcagatc
     6241 agtctctcca aaaaagaggg catagtcgaa caacgggtca agcccacggt catccgccgg
     6301 cgggtaatga aagaagagcc taccccggaa ccgcctcccc caccgccgaa agaagaagta
     6361 aaaccggccg tcgaagccaa gcctcccgtg gccaagaagg gggaagtcag acctccggct
     6421 gtcaagaagg aggggaaacc cgaagcggcc aaagtattgg cggaggcgcc ggtcgaggtt
     6481 ccaaagaaaa aggttgaaat caaagccgaa gctccgtccc ccaaagcaga gggggtagag
     6541 cccggtcccg agcctacagc catccccgtt cccccggtag ttcctccccc cccggtgaaa
     6601 gaggaagaaa agaaagtcga ggcacctgtt gggataaaac cggctcccgg ggctgaaatt
     6661 cctctgaaac ccaaggcagc ggagcccaga ggcgaaggag ttgccccggc ggaaaaaccc
     6721 aagcccccag aaaagccccc caaaccaaag aagaagtatg aaccagcgaa gatcatcgag
     6781 aggccccctg ttccccctcc ctccttagtg aaggagcggg aacatgtccc gatggaaaaa
     6841 agacctgcgg ccccccccag gcctccgatt atcctgcagc gacctactgc ccctgggcca
     6901 gggcggccgg ctcccccagc ggcagcagcc gaagaagaac ggggccggcg gaagaagaag
     6961 ctcaaaaagg atgtggaggt tctcgaagaa ggaaaacccg atcgggttcg ggtgatttcc
     7021 accaagaagc gggcctttaa agaatatgat taccagcggg aagagcaggg agctcggcct
     7081 tttgtctttg aaagagaacc gaaacagcgg gtggtcgccc ggaccaagat ggccaaaccg
     7141 gagattaccg tccccaaagc gataaagaga aagattcgta tttcagaagc catcctggtg
     7201 ggagagctgg ccaaacggat gggggtcaag gtctcagaag tcatcaagaa acttatggga
     7261 ttgggattga tggtgaccat caaccaggcc atcgatgccg atgccgcctc cctggtcgcc
     7321 gccgattttg gttacgaggt ggagaagacc gctttggagc tggaagacct gattgaggag
     7381 cgggaagaca aggcagaaga cctccaaccc cgtccccccg tggtcacgat catggggcac
     7441 gtggaccacg gcaagacctc gcttctggac tccatccggc agaccaatgt gaccgccggg
     7501 gaagcgggcg gaattaccca acatatcgga gcctatacgg tcgatgtgga ccaccgcaag
     7561 gtggtattcc tggatacccc gggtcatgaa gccttcaccg caatgcgcgc ccggggagcc
     7621 aaggttacgg acatcgtcat cctggtcgtg gccgcggatg acggcgtgat gaaccagacc
     7681 gtggaggcca tcaaccacgc caaggccgcc aacgtgccgc tgatcgtcgc cattaacaag
     7741 atcgacaaac ccaacgccaa tccggatcgg gtcaagcaga acatgaccga atatggactg
     7801 gtgcccgaag cctggggcgg ccagaccatc atggccgaga tttcggcgaa acagaaggtc
     7861 gggctccagg agctcctgga gctcatcctc ctccaggcgg aggttctgga gctcagggcc
     7921 aaccccaacc gggccatgac cggaacggtg atcgaggcca aactcgaccg gggccggggc
     7981 ccggtggcca cgattctggt gcaggacgga accctgcatg tgggagattc ctttgtctgc
     8041 gggagctatt tcggacgggt ccgggcgatg atcaatgacc ggggggaaaa gatggaagag
     8101 gccggcccct ccacccccgt ggaggtcatc ggcttttcca gcgttccgca ggccggagaa
     8161 tccttcgtag tagtgaccga tgagaagaag gccaaggatt tggccgctca gaggacgcaa
     8221 aaacagcggg agctggagct ggccaagtct tcccgggtga cccttgagga tttctacaag
     8281 aagatgaaga agggggtggt caaagacctg aacctcatcc tcaaggcgga tgtccaggga
     8341 tcgatcgagg cgctgaccga agcgctgggg agattgagca cggaagccgt ccgggtaaac
     8401 atcctccatt catccgtggg ggcgatcaac gagtcggact gtatgctggc ttcagcctcc
     8461 gaggccatca tcctgggctt cggcgtgaag atggagccca gcgcgcaggg cgtggctgaa
     8521 caggaaaaaa tcgacatccg tttttacaac gtgatctacg acgccatcga ggatgtgcgc
     8581 aaggccatgg aagggctcct ggagccgatt tatgagaagc ggctgacggg caaagccaag
     8641 gtgatccagc ttttcaccat ctctcgggtg ggggtggtcg ccggctccat ggtcttcgag
     8701 ggaaagatcc tccggggctc caaggcccat gtggtccggg acggcaagac gatccacgag
     8761 ggagagattg ccactctgaa acgggtcaag gacgatgtca aggaggtggt ctccggcttt
     8821 gattgcggga tcaccgtggg aggatttcag gattttcagt tgaatgatgt catcgagtcc
     8881 tatgtggtcg aaaaggtgac cgcccatctg tagaagggag aaccccggcc gtggtcatcg
     8941 gcctttgcca tctggaactc ctgctggacg ggaatttttc tctcaaggga aaaagacagg
     9001 tgatcaagag tatcgtggac cgcgcccggc agcgtttcaa catctctatc gccgaggtgg
     9061 aggaccagga cctgtggcag aaggcggtcc tgggggtctg cacggtcgcc aacgaccggc
     9121 agcgggtcaa ctccattctg gacaaggtgg tgagcttcat cgagaacacc aacctggccc
     9181 aggtgaccga ctcgcagatc gaaatcatga acgtttaatt ttcaccacag agacacggag
     9241 gacacagaga aaatataatt cggcaaaaga ataagaccct acggacgaag ggaattcaag
     9301 aagcgtggta ataatactat atatgtcttt ctccgtgttc tctgtgcctc tgtggtgaat
     9361 aggtttttat ggtttacaaa aggtccgacc gggttggcga catgattcgg caggtcatcg
     9421 gggagatgct ccggagagac ctgaatgacc cccggctgag tttggtgact ctaacggaag
     9481 tgaaggtcac ggaagacctc aagattgcca cggtcttctt cagcgccatg ggaaatcccg
     9541 ttcaagaaga agcttcgctc cacggcttgc agagcgcagc cgggtacatc aagaaaaggc
     9601 tgggaaggga actgaggctg agatttgtcc ccgaattgtt gttcaaagtg gatcattcct
     9661 tcgattatgg aaagcggatc gatcggctca ttgaaagtgt ccaagacgaa gaaaaaggag
     9721 atcctccgga ggatcgctga ggaaatccgg gggaggcgca gttttctgat ttccacccat
     9781 cagaatccgg aaggagacgc cattgggtcc gtcctggctc tgggtttggc tttgaagagc
     9841 cttggaaagg aagcgctcat actgactcag gacccggttc cggaggttct ggcctttctt
     9901 cccgggtctg gggagattgt ccatcaggcc ccggtgaacc gcgggtttga catcgccctg
     9961 gccgtggact gcggagaccg cccccggctg ggggaggaat tcagcaaagt gcaggggatc
    10021 ggaaagatca tcaacatcga tcatcacgtg agcaacggct tcttcgggga cttgaacctg
    10081 atcgacccta aggcgagttc cgctgcggag atcatctacg acctgatcca ggccctcccg
    10141 gctcctttca ccagagaggt ggccgagaac atatacacag ggcttctgac cgatactggg
    10201 tctttccatt actccaatac ttccccgaag accttttccg tggcccgggc ttgtatgcta
    10261 gccggggtgg acccctggga agtatcccag aagatatacg atacccaacc cctcccccgg
    10321 cttcgccttt tgccgcgggt tttggatacg ttggagcttt tggaaaatgg acgagtaagc
    10381 tccatggtgg taacccagca gatgctgaag gaaaccgtcg cgaccgtggc catgaccgag
    10441 gacttcatca actttgcccg gtcggtccag ggggtggaag tggccttgct ctttcgggag
    10501 gtgacgtccc ggaaataccg ggttagcatg cgctcgcggg ggcgcgtgga tgtggcccgg
    10561 attgcgggtc aattccaggg agggggacat cccgccgcct ccggctgcac ggtggaggga
    10621 agtttgtccg aagtccggac ccaggtcctg gaagttgtaa aggcggcttt ttgaacgggg
    10681 tcctggtcat cgacaaacct aaaggctgga cctctcacaa agtggtcgcc tgggtgagaa
    10741 atgcgttacg cctccggaag gtgggtcacg gggggactct ggaccctctg gcgacagggg
    10801 ttttgcccat ctatctgggg gagggcacca agctggttcc cttcaacctc gaaggggtca
    10861 aagagtacat cgccacgatg aagctggggc aggagaccga taccttggac gcggatggaa
    10921 gaatcgtggc ggaaaaagaa ggattccttt gtacccggca gactgtagaa gaggttctcc
    10981 atcagttccg ggggaagatc cgccagaggc cgcccctcta ttcggcggtc aagcagcagg
    11041 gagtcccact ttacaaacgg gcccggtccg gggaaacacc ggaagtggcg gaacgagatg
    11101 ccgtcgtaca tgctcttttc ataaaaggtt tctccttccc cctggtgacc ctggagatcg
    11161 tctgcgggcg ggggacgtac attcggagcc tttgcgcgga catgggccgg gtgctcggct
    11221 gcggtgccca cctggtggaa ttgagacgaa cccgctccgg aaggtttggc cttgaccagg
    11281 ccatgagctt ggaagacctt tccaggtgga tcgaggaaaa gaggattgag gaaaaggtga
    11341 tccctctgaa aaacggtgta gacctttcgg gggaggtccg gctaggagaa aaggccgcgg
    11401 ccagggtaag acaaggtcaa tccctttgcc tgaacgatct tccggaaggc gaacggaaga
    11461 ggttgcagaa agggcaacgg tggggccttt ttcaggaggc ccagggtctt gtggccattg
    11521 ccgagtcgca ggtggatgct gaatcccagc tttccgggga ttcccaggcc ttgcgcatcc
    11581 tccgggtctt tcagacatga atgataaaga attttcgttc attgtcccat ggtgagtaaa
    11641 agaatattag ccgagcaaat tcggattctg aataggaagc tagaaaggag aatgaggtga
    11701 cactactgcc agagcggaag aaggccatca tcgaggggaa taagagacac cctacggaca
    11761 cgggatctcc cgaggtccag gtcgccatcc taagcgagcg gatcaattac ctgacggagc
    11821 attttaaggc ccatacgaag gaccatcatt cccgcagggg gctcttgcag ctcgtcggcc
    11881 agaggcggca cctcctcgac tatctcaaga agaaggatgt ggagcgctac cgctccctga
    11941 tccagaaact gggaatccgg aaataagaaa gattcagggg tgccgcaata aactccattt
    12001 tgttgcaccc cgaaaaggcc tgcccggttc gagggcgggg gaagaaggac atcttttgga
    12061 aagaggttga gtttatgtct acacttggaa atcgcctcga ggttcgctgt aacctgggag
    12121 gcagggattt tatttttgaa acaggggctg tggccaaaca agcggatgga gcggtcctgg
    12181 ttacctttgg agagagtatg gtcctggtca cggcgacggc ctccagaacc attagggaag
    12241 ggatcgattt cttccccctg gttgtcgact accaggagat ggcctacgcg gcgggaaaga
    12301 tccccggcgg atttttcaaa cgcgaaggtc ggcccagcga gaaagagatt cttaccgccc
    12361 ggctgatcga tcggcccttg cgtcccctct ttcccaaagg tttcttcaac gaagtccaga
    12421 tcgtcgccac ggtcctgtca gcggatcagg agaatgaccc cgatgttctg gccatctgcg
    12481 gagcctcgac ggccctgcag atttcggaca ttcccttcca gggccccatc gccggagtcc
    12541 gggtggggag gatccagggc cagtgggtct gcaaccccac cggaagccag ctcgcccaga
    12601 gcgacgtgaa tctggtggtg gccgggagcc gggaaggggt ggtcatggtg gaaggcgggg
    12661 gccaggagat ttccgaagag gaactgctgg aggcgatctt tttcggccac cgctccctgc
    12721 agcccatcct ggatatccag gaagaattga aaaagaaaaa gatcgagcag gaaaacgaac
    12781 agcgaagggc tgccggagaa gcggagatcg ccgacatcgt aaagaggcgc gctcctcagc
    12841 ttttcccgga cccggtcctg gggaaatccg tgctgctggc cgatgaatcg atccgggaac
    12901 gggtccgcac agccgggcag gaaaagattc ttcaggccct gtccatttct aagaagaaag
    12961 tgcgcaccga acaactggag caggcgaggg aggaaaccct gaaggcactt ctggaagagt
    13021 tccccgaaag ggcgggagag atcaaagcct attacgaggc ggtagagaaa tttgtcgtcc
    13081 gccggcccat cctccaagag aaccgccggg tggacgggcg acgtttcaac gaagtccggc
    13141 ccattcacat ccaggtcggc tggctgccga gaactcacgg atcggcccgc ttcacccgcg
    13201 gagagacgca ggtctccgcg gtagtcaccc tgggatcggc ggaggacgag cagaagatcg
    13261 acgcccttac cggggagact tttaagtcct tcatgctcca ttataatttc cccccttata
    13321 gcgtcggcga agtccgcatg ctacgggggc ccagccgccg ggacatcggc catggagctt
    13381 tggccgaacg gtccatcaac cgcattctgc cgcccagcga taaattcccc tataccgtcc
    13441 ggatcgtctc cgaagtcctg gaatccaacg ggtcctcttc catggccacc gtctgcgggt
    13501 cttccctggc cttgatggat gccggcgttc agatcaaacg gccggtggct gggatcgcca
    13561 tgggtttgat caaagaggga gagaaggtgg tcgttcttac ggatatcctg ggagacgaag
    13621 accatcacgg agacatggat ttcaaggtgg ccgggtcccg gagcggcatt accggattcc
    13681 agatggacat caaaatttcc ggggtgaccc gggagatcct ccgtcaggct ctggcccaag
    13741 cacgagaagg aagggagttc attttgcaga aaatggagga agcgttagcc gggccgagag
    13801 tcgagatgtc tccctatgct ccccgcttcc tcagcctgag aatcaaaccc gaccggatcc
    13861 gggagatcat cggaccccag ggaaaagtca tccgggggat tcaggatgag accggggtaa
    13921 agatcaatgt ggaagacgat ggatcggttc atatcttctc gcccgattcc caggcggccc
    13981 agaaagcggc gaacatcatc cgcgatatga tcaaggaagc tgaagtgggg caattctaca
    14041 tgggccgggt cacctccatc gcccgcaagc aggatggaaa ggagttcgga gccttcgtgg
    14101 agattttccc gggaaccgac gggctggttc acatctccca actggccaaa gaaagggtcc
    14161 gcaacgtgga ggatgtgctg aaagaaggag accaggtcct ggtcaaagtc atcggaatcg
    14221 acgaacgggg aaaaatcaag ctcagccgga aagaggccct aggccatcct tggccggaga
    14281 acaaat
//
